LOCUS       DAZGJW010000001       113010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZGJW010000001 DAZGJW010000000
VERSION     DAZGJW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 113010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 113010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..113010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.001"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            42..1331
                     /gene="uraA"
                     /locus_tag="ACHKIK_000001"
     CDS             42..1331
                     /gene="uraA"
                     /locus_tag="ACHKIK_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO9081252.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHHSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            1426..2127
                     /gene="hda"
                     /locus_tag="ACHKIK_000002"
     CDS             1426..2127
                     /gene="hda"
                     /locus_tag="ACHKIK_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO9081253.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(2265..2624)
                     /gene="arsC"
                     /locus_tag="ACHKIK_000003"
     CDS             complement(2265..2624)
                     /gene="arsC"
                     /locus_tag="ACHKIK_000003"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO9081254.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2645..4108)
                     /gene="bepA"
                     /locus_tag="ACHKIK_000004"
     CDS             complement(2645..4108)
                     /gene="bepA"
                     /locus_tag="ACHKIK_000004"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO9081255.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            4321..5382
                     /gene="yfgO"
                     /locus_tag="ACHKIK_000005"
     CDS             4321..5382
                     /gene="yfgO"
                     /locus_tag="ACHKIK_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO9081256.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(5420..6268)
                     /gene="focB"
                     /locus_tag="ACHKIK_000006"
     CDS             complement(5420..6268)
                     /gene="focB"
                     /locus_tag="ACHKIK_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO9081257.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(6290..8302)
                     /gene="hyfR"
                     /locus_tag="ACHKIK_000007"
     CDS             complement(6290..8302)
                     /gene="hyfR"
                     /locus_tag="ACHKIK_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO9081258.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(8332..8745)
                     /gene="hyfJ"
                     /locus_tag="ACHKIK_000008"
     CDS             complement(8332..8745)
                     /gene="hyfJ"
                     /locus_tag="ACHKIK_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO9081259.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(8738..9495)
                     /gene="hyfI"
                     /locus_tag="ACHKIK_000009"
                     /pseudo
     CDS             complement(8738..9495)
                     /gene="hyfI"
                     /locus_tag="ACHKIK_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(9492..10037)
                     /gene="hyfH"
                     /locus_tag="ACHKIK_000010"
     CDS             complement(9492..10037)
                     /gene="hyfH"
                     /locus_tag="ACHKIK_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO9081260.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(10047..11714)
                     /gene="hyfG"
                     /locus_tag="ACHKIK_000011"
     CDS             complement(10047..11714)
                     /gene="hyfG"
                     /locus_tag="ACHKIK_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO9081261.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDHRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDAQVIPVGPLHITSDELGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(11704..13284)
                     /gene="hyfF"
                     /locus_tag="ACHKIK_000012"
     CDS             complement(11704..13284)
                     /gene="hyfF"
                     /locus_tag="ACHKIK_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO9081262.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(13289..13939)
                     /gene="hyfE"
                     /locus_tag="ACHKIK_000013"
     CDS             complement(13289..13939)
                     /gene="hyfE"
                     /locus_tag="ACHKIK_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO9081263.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(13951..15390)
                     /gene="hyfD"
                     /locus_tag="ACHKIK_000014"
     CDS             complement(13951..15390)
                     /gene="hyfD"
                     /locus_tag="ACHKIK_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFO9081264.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLLFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASKVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(15407..16354)
                     /gene="hyfC"
                     /locus_tag="ACHKIK_000015"
     CDS             complement(15407..16354)
                     /gene="hyfC"
                     /locus_tag="ACHKIK_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO9081265.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLSFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(16365..18383)
                     /gene="hyfB"
                     /locus_tag="ACHKIK_000016"
     CDS             complement(16365..18383)
                     /gene="hyfB"
                     /locus_tag="ACHKIK_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFO9081266.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADFTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGFAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(18383..19000)
                     /gene="hyfA"
                     /locus_tag="ACHKIK_000017"
     CDS             complement(18383..19000)
                     /gene="hyfA"
                     /locus_tag="ACHKIK_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO9081267.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(19253..19723)
                     /gene="bcp"
                     /locus_tag="ACHKIK_000018"
     CDS             complement(19253..19723)
                     /gene="bcp"
                     /locus_tag="ACHKIK_000018"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO9081268.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(19723..20295)
                     /gene="gcvR"
                     /locus_tag="ACHKIK_000019"
     CDS             complement(19723..20295)
                     /gene="gcvR"
                     /locus_tag="ACHKIK_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO9081269.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            20441..21319
                     /gene="dapA"
                     /locus_tag="ACHKIK_000020"
     CDS             20441..21319
                     /gene="dapA"
                     /locus_tag="ACHKIK_000020"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO9081270.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            21336..22370
                     /gene="bamC"
                     /locus_tag="ACHKIK_000021"
     CDS             21336..22370
                     /gene="bamC"
                     /locus_tag="ACHKIK_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO9081271.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            22583..23296
                     /gene="purC"
                     /locus_tag="ACHKIK_000022"
     CDS             22583..23296
                     /gene="purC"
                     /locus_tag="ACHKIK_000022"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO9081272.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            23450..23677
                     /gene="vapB"
                     /locus_tag="ACHKIK_000023"
     CDS             23450..23677
                     /gene="vapB"
                     /locus_tag="ACHKIK_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFO9081273.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            23677..24075
                     /locus_tag="ACHKIK_000024"
     CDS             23677..24075
                     /locus_tag="ACHKIK_000024"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO9081274.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            24222..25085
                     /gene="ypfJ"
                     /locus_tag="ACHKIK_000025"
     CDS             24222..25085
                     /gene="ypfJ"
                     /locus_tag="ACHKIK_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO9081275.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            25100..27115
                     /gene="tmcA"
                     /locus_tag="ACHKIK_000026"
     CDS             25100..27115
                     /gene="tmcA"
                     /locus_tag="ACHKIK_000026"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO9081276.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            27189..27887
                     /gene="ypfH"
                     /locus_tag="ACHKIK_000027"
     CDS             27189..27887
                     /gene="ypfH"
                     /locus_tag="ACHKIK_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO9081277.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(27997..28197)
                     /gene="ypfN"
                     /locus_tag="ACHKIK_000028"
     CDS             complement(27997..28197)
                     /gene="ypfN"
                     /locus_tag="ACHKIK_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO9081278.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(28225..29352)
                     /gene="dapE"
                     /locus_tag="ACHKIK_000029"
     CDS             complement(28225..29352)
                     /gene="dapE"
                     /locus_tag="ACHKIK_000029"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO9081279.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAANLQLLARMYQRIMEQLVA"
     gene            complement(29356..29712)
                     /gene="yffB"
                     /locus_tag="ACHKIK_000030"
     CDS             complement(29356..29712)
                     /gene="yffB"
                     /locus_tag="ACHKIK_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO9081280.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            30093..30152
                     /gene="ypfM"
                     /locus_tag="ACHKIK_000031"
     CDS             30093..30152
                     /gene="ypfM"
                     /locus_tag="ACHKIK_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO9081281.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(30251..33364)
                     /gene="acrD"
                     /locus_tag="ACHKIK_000032"
     CDS             complement(30251..33364)
                     /gene="acrD"
                     /locus_tag="ACHKIK_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO9081282.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(33528..35228)
                     /gene="narQ"
                     /locus_tag="ACHKIK_000033"
     CDS             complement(33528..35228)
                     /gene="narQ"
                     /locus_tag="ACHKIK_000033"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO9081283.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            35434..37413
                     /gene="aegA"
                     /locus_tag="ACHKIK_000034"
     CDS             35434..37413
                     /gene="aegA"
                     /locus_tag="ACHKIK_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO9081284.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKPRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMNAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            37481..38056
                     /gene="nudK"
                     /locus_tag="ACHKIK_000035"
     CDS             37481..38056
                     /gene="nudK"
                     /locus_tag="ACHKIK_000035"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO9081285.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            38182..39225
                     /gene="ypfG"
                     /locus_tag="ACHKIK_000036"
     CDS             38182..39225
                     /gene="ypfG"
                     /locus_tag="ACHKIK_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO9081286.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQVIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(39321..41324)
                     /gene="tkt"
                     /locus_tag="ACHKIK_000037"
     CDS             complement(39321..41324)
                     /gene="tkt"
                     /locus_tag="ACHKIK_000037"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO9081287.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSMKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGVIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(41344..42294)
                     /gene="tal"
                     /locus_tag="ACHKIK_000038"
     CDS             complement(41344..42294)
                     /gene="tal"
                     /locus_tag="ACHKIK_000038"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO9081288.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            42583..44862
                     /gene="maeB"
                     /locus_tag="ACHKIK_000039"
     CDS             42583..44862
                     /gene="maeB"
                     /locus_tag="ACHKIK_000039"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO9081289.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            45155..45490
                     /gene="eutS"
                     /locus_tag="ACHKIK_000040"
     CDS             45155..45490
                     /gene="eutS"
                     /locus_tag="ACHKIK_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO9081290.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            45503..45982
                     /gene="eutP"
                     /locus_tag="ACHKIK_000041"
     CDS             45503..45982
                     /gene="eutP"
                     /locus_tag="ACHKIK_000041"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO9081291.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            45957..46658
                     /gene="eutQ"
                     /locus_tag="ACHKIK_000042"
     CDS             45957..46658
                     /gene="eutQ"
                     /locus_tag="ACHKIK_000042"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO9081292.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            46655..47458
                     /gene="eutT"
                     /locus_tag="ACHKIK_000043"
     CDS             46655..47458
                     /gene="eutT"
                     /locus_tag="ACHKIK_000043"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO9081293.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            47455..48471
                     /gene="pta"
                     /locus_tag="ACHKIK_000044"
     CDS             47455..48471
                     /gene="pta"
                     /locus_tag="ACHKIK_000044"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO9081294.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            48510..48803
                     /gene="eutM"
                     /locus_tag="ACHKIK_000045"
     CDS             48510..48803
                     /gene="eutM"
                     /locus_tag="ACHKIK_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO9081295.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            48910..49197
                     /gene="eutN"
                     /locus_tag="ACHKIK_000046"
     CDS             48910..49197
                     /gene="eutN"
                     /locus_tag="ACHKIK_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO9081296.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            49209..50612
                     /gene="eutE"
                     /locus_tag="ACHKIK_000047"
     CDS             49209..50612
                     /gene="eutE"
                     /locus_tag="ACHKIK_000047"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO9081297.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            50623..51459
                     /gene="eutJ"
                     /locus_tag="ACHKIK_000048"
     CDS             50623..51459
                     /gene="eutJ"
                     /locus_tag="ACHKIK_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO9081298.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGLRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            51449..52636
                     /gene="eutG"
                     /locus_tag="ACHKIK_000049"
     CDS             51449..52636
                     /gene="eutG"
                     /locus_tag="ACHKIK_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO9081299.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQSRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            52753..53979
                     /gene="eutH"
                     /locus_tag="ACHKIK_000050"
     CDS             52753..53979
                     /gene="eutH"
                     /locus_tag="ACHKIK_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO9081300.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            53976..55379
                     /gene="eutA"
                     /locus_tag="ACHKIK_000051"
     CDS             53976..55379
                     /gene="eutA"
                     /locus_tag="ACHKIK_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO9081301.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLMETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            55391..56752
                     /gene="eutB"
                     /locus_tag="ACHKIK_000052"
     CDS             55391..56752
                     /gene="eutB"
                     /locus_tag="ACHKIK_000052"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO9081302.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            56773..57660
                     /gene="eutC"
                     /locus_tag="ACHKIK_000053"
     CDS             56773..57660
                     /gene="eutC"
                     /locus_tag="ACHKIK_000053"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO9081303.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            57670..58329
                     /gene="eutL"
                     /locus_tag="ACHKIK_000054"
     CDS             57670..58329
                     /gene="eutL"
                     /locus_tag="ACHKIK_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO9081304.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            58342..58842
                     /gene="eutK"
                     /locus_tag="ACHKIK_000055"
     CDS             58342..58842
                     /gene="eutK"
                     /locus_tag="ACHKIK_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO9081305.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            58888..59940
                     /gene="eutR"
                     /locus_tag="ACHKIK_000056"
     CDS             58888..59940
                     /gene="eutR"
                     /locus_tag="ACHKIK_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO9081306.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHCEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(59946..60845)
                     /gene="hemF"
                     /locus_tag="ACHKIK_000057"
     CDS             complement(59946..60845)
                     /gene="hemF"
                     /locus_tag="ACHKIK_000057"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO9081307.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERECNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(60849..61718)
                     /gene="amiA"
                     /locus_tag="ACHKIK_000058"
     CDS             complement(60849..61718)
                     /gene="amiA"
                     /locus_tag="ACHKIK_000058"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO9081308.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            61932..62357
                     /gene="ypeA"
                     /locus_tag="ACHKIK_000059"
     CDS             61932..62357
                     /gene="ypeA"
                     /locus_tag="ACHKIK_000059"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO9081309.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            62344..62793
                     /gene="yfeZ"
                     /locus_tag="ACHKIK_000060"
     CDS             62344..62793
                     /gene="yfeZ"
                     /locus_tag="ACHKIK_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO9081310.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            62854..63429
                     /gene="yfeY"
                     /locus_tag="ACHKIK_000061"
     CDS             62854..63429
                     /gene="yfeY"
                     /locus_tag="ACHKIK_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO9081311.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            63525..64424
                     /gene="yfeX"
                     /locus_tag="ACHKIK_000062"
     CDS             63525..64424
                     /gene="yfeX"
                     /locus_tag="ACHKIK_000062"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO9081312.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(64482..65786)
                     /gene="pbp4b"
                     /locus_tag="ACHKIK_000063"
     CDS             complement(64482..65786)
                     /gene="pbp4b"
                     /locus_tag="ACHKIK_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO9081313.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(65791..67215)
                     /gene="murP"
                     /locus_tag="ACHKIK_000064"
     CDS             complement(65791..67215)
                     /gene="murP"
                     /locus_tag="ACHKIK_000064"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO9081314.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(67219..68115)
                     /gene="murQ"
                     /locus_tag="ACHKIK_000065"
     CDS             complement(67219..68115)
                     /gene="murQ"
                     /locus_tag="ACHKIK_000065"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO9081315.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            68279..69136
                     /gene="murR"
                     /locus_tag="ACHKIK_000066"
     CDS             68279..69136
                     /gene="murR"
                     /locus_tag="ACHKIK_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO9081316.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            69265..70056
                     /gene="ucpA"
                     /locus_tag="ACHKIK_000067"
     CDS             69265..70056
                     /gene="ucpA"
                     /locus_tag="ACHKIK_000067"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO9081317.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTDMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            70227..71243
                     /gene="cysP"
                     /locus_tag="ACHKIK_000068"
     CDS             70227..71243
                     /gene="cysP"
                     /locus_tag="ACHKIK_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO9081318.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            71243..72076
                     /gene="cysT"
                     /locus_tag="ACHKIK_000069"
     CDS             71243..72076
                     /gene="cysT"
                     /locus_tag="ACHKIK_000069"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO9081319.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            72076..72951
                     /gene="cysW"
                     /locus_tag="ACHKIK_000070"
     CDS             72076..72951
                     /gene="cysW"
                     /locus_tag="ACHKIK_000070"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO9081320.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQDKRAQQEEHHEH"
     gene            72941..74038
                     /gene="cysA"
                     /locus_tag="ACHKIK_000071"
     CDS             72941..74038
                     /gene="cysA"
                     /locus_tag="ACHKIK_000071"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO9081321.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            74172..75083
                     /gene="cysM"
                     /locus_tag="ACHKIK_000072"
     CDS             74172..75083
                     /gene="cysM"
                     /locus_tag="ACHKIK_000072"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO9081322.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(75086..75454)
                     /gene="yfeK"
                     /locus_tag="ACHKIK_000073"
     CDS             complement(75086..75454)
                     /gene="yfeK"
                     /locus_tag="ACHKIK_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO9081323.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            75559..76410
                     /gene="pdxK"
                     /locus_tag="ACHKIK_000074"
                     /gene_synonym="thiJ"
     CDS             75559..76410
                     /gene="pdxK"
                     /locus_tag="ACHKIK_000074"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO9081324.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(76452..76961)
                     /gene="crr"
                     /locus_tag="ACHKIK_000075"
     CDS             complement(76452..76961)
                     /gene="crr"
                     /locus_tag="ACHKIK_000075"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO9081325.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(77002..78729)
                     /gene="ptsI"
                     /locus_tag="ACHKIK_000076"
     CDS             complement(77002..78729)
                     /gene="ptsI"
                     /locus_tag="ACHKIK_000076"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO9081326.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(78774..79031)
                     /gene="ptsH"
                     /locus_tag="ACHKIK_000077"
     CDS             complement(78774..79031)
                     /gene="ptsH"
                     /locus_tag="ACHKIK_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO9081327.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(79415..80386)
                     /gene="cysK"
                     /locus_tag="ACHKIK_000078"
     CDS             complement(79415..80386)
                     /gene="cysK"
                     /locus_tag="ACHKIK_000078"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO9081328.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(80571..81332)
                     /gene="cysZ"
                     /locus_tag="ACHKIK_000079"
     CDS             complement(80571..81332)
                     /gene="cysZ"
                     /locus_tag="ACHKIK_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO9081329.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            81562..82548
                     /gene="zipA"
                     /locus_tag="ACHKIK_000080"
     CDS             81562..82548
                     /gene="zipA"
                     /locus_tag="ACHKIK_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO9081330.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            82619..84634
                     /gene="ligA"
                     /locus_tag="ACHKIK_000081"
     CDS             82619..84634
                     /gene="ligA"
                     /locus_tag="ACHKIK_000081"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO9081331.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            84636..84854
                     /gene="ypeB"
                     /locus_tag="ACHKIK_000082"
     CDS             84636..84854
                     /gene="ypeB"
                     /locus_tag="ACHKIK_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO9081332.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(84851..85849)
                     /gene="yfeH"
                     /locus_tag="ACHKIK_000083"
     CDS             complement(84851..85849)
                     /gene="yfeH"
                     /locus_tag="ACHKIK_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO9081333.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            85939..86865
                     /gene="yfeR"
                     /locus_tag="ACHKIK_000084"
     CDS             85939..86865
                     /gene="yfeR"
                     /locus_tag="ACHKIK_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9081334.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(86904..87668)
                     /gene="yfeN"
                     /locus_tag="ACHKIK_000085"
     CDS             complement(86904..87668)
                     /gene="yfeN"
                     /locus_tag="ACHKIK_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO9081335.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            87917..88750
                     /gene="xapA"
                     /locus_tag="ACHKIK_000086"
     CDS             87917..88750
                     /gene="xapA"
                     /locus_tag="ACHKIK_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFO9081336.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            88810..90066
                     /gene="xapB"
                     /locus_tag="ACHKIK_000087"
     CDS             88810..90066
                     /gene="xapB"
                     /locus_tag="ACHKIK_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO9081337.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            90119..90277
                     /locus_tag="ACHKIK_000088"
     CDS             90119..90277
                     /locus_tag="ACHKIK_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFO9081338.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            90318..91202
                     /gene="xapR"
                     /locus_tag="ACHKIK_000089"
     CDS             90318..91202
                     /gene="xapR"
                     /locus_tag="ACHKIK_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO9081339.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(91311..91436)
                     /locus_tag="ACHKIK_000090"
     ncRNA           complement(91311..91436)
                     /ncRNA_class="other"
                     /locus_tag="ACHKIK_000090"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(91468..91543)
                     /locus_tag="ACHKIK_000091"
     tRNA            complement(91468..91543)
                     /locus_tag="ACHKIK_000091"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91508..91510),aa:Lys,seq:ttt)
     gene            complement(91548..91623)
                     /locus_tag="ACHKIK_000092"
     tRNA            complement(91548..91623)
                     /locus_tag="ACHKIK_000092"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91588..91590),aa:Val,seq:tac)
     gene            complement(91670..91745)
                     /locus_tag="ACHKIK_000093"
     tRNA            complement(91670..91745)
                     /locus_tag="ACHKIK_000093"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91710..91712),aa:Val,seq:tac)
     gene            complement(91790..91865)
                     /locus_tag="ACHKIK_000094"
     tRNA            complement(91790..91865)
                     /locus_tag="ACHKIK_000094"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91830..91832),aa:Val,seq:tac)
     gene            92124..93539
                     /gene="gltX"
                     /locus_tag="ACHKIK_000095"
     CDS             92124..93539
                     /gene="gltX"
                     /locus_tag="ACHKIK_000095"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO9081340.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(93591..93983)
                     /gene="yfeD"
                     /locus_tag="ACHKIK_000096"
     CDS             complement(93591..93983)
                     /gene="yfeD"
                     /locus_tag="ACHKIK_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO9081341.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(93985..94344)
                     /gene="yfeC"
                     /locus_tag="ACHKIK_000097"
     CDS             complement(93985..94344)
                     /gene="yfeC"
                     /locus_tag="ACHKIK_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO9081342.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            94565..94640
                     /locus_tag="ACHKIK_000098"
     tRNA            94565..94640
                     /locus_tag="ACHKIK_000098"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:94598..94600,aa:Ala,seq:ggc)
     gene            94680..94755
                     /locus_tag="ACHKIK_000099"
     tRNA            94680..94755
                     /locus_tag="ACHKIK_000099"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:94713..94715,aa:Ala,seq:ggc)
     gene            94963..97152
                     /gene="pdeA"
                     /locus_tag="ACHKIK_000100"
     CDS             94963..97152
                     /gene="pdeA"
                     /locus_tag="ACHKIK_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO9081343.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGDGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(97202..98404)
                     /gene="nupC"
                     /locus_tag="ACHKIK_000101"
     CDS             complement(97202..98404)
                     /gene="nupC"
                     /locus_tag="ACHKIK_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO9081344.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            98740..99978
                     /gene="mntH"
                     /locus_tag="ACHKIK_000102"
     CDS             98740..99978
                     /gene="mntH"
                     /locus_tag="ACHKIK_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO9081345.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(100119..100445)
                     /gene="ypeC"
                     /locus_tag="ACHKIK_000103"
     CDS             complement(100119..100445)
                     /gene="ypeC"
                     /locus_tag="ACHKIK_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO9081346.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(100560..101816)
                     /gene="yfeO"
                     /locus_tag="ACHKIK_000104"
     CDS             complement(100560..101816)
                     /gene="yfeO"
                     /locus_tag="ACHKIK_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO9081347.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            102020..102985
                     /gene="glk"
                     /locus_tag="ACHKIK_000105"
     CDS             102020..102985
                     /gene="glk"
                     /locus_tag="ACHKIK_000105"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO9081348.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVYDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            103204..103530
                     /gene="fryB"
                     /locus_tag="ACHKIK_000106"
     CDS             103204..103530
                     /gene="fryB"
                     /locus_tag="ACHKIK_000106"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO9081349.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            103552..104799
                     /gene="fryC"
                     /locus_tag="ACHKIK_000107"
     CDS             103552..104799
                     /gene="fryC"
                     /locus_tag="ACHKIK_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO9081350.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKGLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            104814..105899
                     /gene="ypdF"
                     /locus_tag="ACHKIK_000108"
     CDS             104814..105899
                     /gene="ypdF"
                     /locus_tag="ACHKIK_000108"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO9081351.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            105899..106936
                     /gene="ypdE"
                     /locus_tag="ACHKIK_000109"
     CDS             105899..106936
                     /gene="ypdE"
                     /locus_tag="ACHKIK_000109"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO9081352.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            106961..109456
                     /gene="ptsP"
                     /locus_tag="ACHKIK_000110"
     CDS             106961..109456
                     /gene="ptsP"
                     /locus_tag="ACHKIK_000110"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO9081353.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILTRASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(109459..110316)
                     /gene="ypdC"
                     /locus_tag="ACHKIK_000111"
     CDS             complement(109459..110316)
                     /gene="ypdC"
                     /locus_tag="ACHKIK_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9081354.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(110329..111063)
                     /gene="ypdB"
                     /locus_tag="ACHKIK_000112"
     CDS             complement(110329..111063)
                     /gene="ypdB"
                     /locus_tag="ACHKIK_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO9081355.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFANKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(111078..112775)
                     /gene="ypdA"
                     /locus_tag="ACHKIK_000113"
     CDS             complement(111078..112775)
                     /gene="ypdA"
                     /locus_tag="ACHKIK_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO9081356.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT        26842 a        28807 c        30705 g        26656 t
ORIGIN      
        1 tgagtaaagc gcctataaca cataatacag aggataatac tatgacgcgc cgtgctatcg
       61 gggtgagtga aagaccgcca cttttacaga caatcccgct tagtttgcaa catttgttcg
      121 ccatgtttgg tgcaaccgtc ctggtgcccg tcttatttca tattaacccg gcgactgtac
      181 tgttatttaa cggtattgga acgctgctgt atctcttcat ctgtaaaggg aaaattccgg
      241 cttatcttgg ttccagcttt gcctttattt caccggtatt gttactgttg ccgttagggt
      301 atgaagtcgc gctgggcggc tttattatgt gcggcgtgct gttctgcctg gtttctttta
      361 tcgtgaagaa agcggggacc ggctggctgg acgtgctgtt tccacctgcg gcaatgggcg
      421 caatcgttgc cgtcatcggt ctggagctgg cgggcgtagc tgccggtatg gcgggtttac
      481 tcccggctga agggcaaacg ccagactcca aaaccatcat catctctatt accaccctgg
      541 cggtcacggt tttaggttcc gtgctgtttc gtggtttcct ggcaattatc ccgattttaa
      601 ttggcgtgct ggtggggtac gcgctctctt tcgcaatggg aattgtcgat accacgccga
      661 ttattaatgc tcactggttt gcgctgccaa ccctctatac gccgcgcttc gagtggtttg
      721 ccattctgac tattctgcca gcggcgttag tggttattgc cgaacacgta gggcacctgg
      781 tagtaacggc taatatcgtc aaaaaagatc tgctgcgcga tccaggtctg caccattcga
      841 tgtttgctaa tggcttgtcg accgtgattt ccggcttctt tggctctacg ccaaatacca
      901 cttacggaga aaacattggc gtgatggcga tcacccgtgt ttacagtacc tgggttatcg
      961 gcggggcggc gattttcgct atcctgcttt cctgcgtcgg taaactggct gccgctatcc
     1021 agatgatccc attgccggtg atgggcggcg tttcgctgct gctttatggt gtcatcggtg
     1081 cttccggtat tcgtgttttg atcgaatcga aagtggatta caacaaagca cagaacctga
     1141 tcctgacttc cgtgattttg atcatcggcg tcagtggcgc gaaggtaaac atcggcgcgg
     1201 cggagttaaa aggtatggcg ctggcgacca tcgtcggtat cggcttaagt ttgatcttca
     1261 aactgatcag tgtgctgcgc cctgaagaag tggtgctgga cgcagaagac gcggatataa
     1321 cagacaaata atgtaccgac cgggcagtgt tgctgcccgg ttcaaacatc atgggattct
     1381 gtggtaaact tctcgcgatt ttgtgaaatc ctggttgagg tatctctgaa cacaccggca
     1441 cagctctctt tgccacttta tcttcctgac gacgaaacct ttgcaagttt ctggccgggg
     1501 gataactcct ctttactggc tgcgctgcaa aacgtgctgc gtcaggaaca tagcggttac
     1561 atctatctct gggcacgcga aggcgcgggg cgcagccatc tgctgcacgc ggcttgcgcg
     1621 gaattgtcgc agcgtggcga tgcggtgggc tatgtcccgc tggataaacg cacctggttt
     1681 gttccggaag tgctcgacgg tatggagcat ttgtcgctgg tctgtatcga caacattgag
     1741 tgtattgcag gcgatgagtt gtgggagatg gcgattttcg atctctacaa tcgaattctg
     1801 gaatcgggca aaacacgatt gttgatcacc ggcgatcgtc caccgcggca gttgaatctg
     1861 ggattaccgg atctcgcgtc gcgactcgac tgggggcaga tctacaaatt gcagccactt
     1921 tctgatgaag ataagttgca ggcgctacag ttacgcgcgc gtttgcgtgg ttttgaactg
     1981 ccggaagatg tggggcgttt cttgctgaag cggctcgaca gagaaatgcg cacgctattt
     2041 atgacgttgg atcagttgga tcgtgcgtcg attaccgcgc aacgtaagct gaccattccg
     2101 tttgtgaaag aaattctgaa gttgtagata aggtgtttat tgtcggatgc ggcgcgaacg
     2161 ccttatccga actacgcgaa gttaatccat agtattcgta ggccggataa gacgtggcaa
     2221 gcgtcgcatc cggcaatgtg cttcaactag cgatggagca catcttaacc cacgatttcc
     2281 agtacctgct ccggtggacg accaatccgc gctttgccat tcgcgaccac aatcgggcgc
     2341 tccattagct tcggattatc caccattgcc tgaatcagcg cctcttcgct aagcgaactg
     2401 tcagccaggt tcagttcttt ataaagatcc tctttctggc gcatcaattc acgggcgcta
     2461 ttcatcccca gcattttcag caaatcgcgc agcgttgccg catcggcggg tgtctcaagg
     2521 tagagtacca cttctggctc cacgccgttt tctttcagca ggttcagcgt ttcccggctc
     2581 tttgaacagc gtgggttatg gtagattttt acctgtttgg tcattgttct tcctttaatg
     2641 cgaattacat cttggtataa ggcttaaagc gttcctgcag ctggcgcaac tggtcgatgc
     2701 gcgcatcgta acgcgcttgt tgcaggctgc ctaatttcac ctgcgaactg gcgctactca
     2761 acagcgaaat ggcctgatcg agtcgtccgg cgagcgcata accttctgct cgcgcagcca
     2821 gctcctgatc gcggttattt agcgcggctt ccgcctgtgc cagtaaatcc cagccgttgc
     2881 tgtcatcttt attattaaag gtgtagcgat tcagaatatt ggccgcttct tgtggttgac
     2941 cgccttgtag ataagcgttc gccaggttga gctgcaacac cggattggtg cgcaaatcac
     3001 gggcattttt caggcgattg attgcctcat tggctttgtt ttgcccaaga tcgatatcgg
     3061 tagccagatc gagataccat gcgttgccag gttctgccgc cagtaacggt tgcagcgttt
     3121 tacgcgcctc gtcgtattta ttggcttcca tcgcctgtaa agcacgacca tattgcgccg
     3181 ctcgttgctg acgaacgttt cctttcgccc attcatccag caaatcactg gtgagctggt
     3241 tacgtccgga attatacatc cccagtgtgc gcgctttcgc cagatagaaa tcttccgacg
     3301 actgcaccac catcggtcgc atctgattag cacggttgcg ggcatctgcc agacgacttt
     3361 ccggcaacgg gtgagtcaat aaaatttccg gcgggcgcga ggagtaacgc gcctgatcga
     3421 gtaatttttc gaggaaggtt ggcatcgcct gcggatcgaa tcccgagcgt tgcagcacct
     3481 gaataccaat gcggtccgct tcctgttcat tttgctgggt gaaactgatc attccctgac
     3541 gtgttcccgc cagtgtaccg gtcagcgccg ccatccccgc ctgcggactg gccatcgcca
     3601 gtaaaataga acctaacgcg ccgacccagg tcagcggcgc gctgcgttgt tgatcttcca
     3661 tcgctcgcgc caggtgacgt tgggtgacgt gggagatttc gtgcgccata actgaagcca
     3721 gttgactttc gttatcggaa taacggaaca gggcagagtg cagcaccacg ttgccgccaa
     3781 agaaagcaaa ggcgttaatt tcgtcgttgt tgatcagaaa aaaatgaaac ggtgtcttaa
     3841 ccgaattggc atgcgaaacc agacgcatcc ccagcgaatt aatatattgc gttaacagcg
     3901 ggtcattaat taacggcgcg ctgccgcgta gctggcggac ataatagtcg cccatctgca
     3961 tttcctgacc aatggaaagc gtgcttcctg cggaggttcc catatccggc aaggtgtctg
     4021 cgctgtcggc aaacgccggg gctacctgac caatggtcat agcagcaatg agggttgcaa
     4081 ccaggttttt tttcaactgc ctgaacataa cctctatcct gtatttctga agatagtcat
     4141 ttgaccgata ctgttaaata tcgttcccac cgcgccgcct ttgagtgtaa cggcgaagat
     4201 tagcccactc aaatgacttt tcagtttcct gaatccgtca taaaaagcga agtctttttt
     4261 gtgacatttc gatacaattg cggatcgcaa tcccgctctt gaggttcagg gaaggttttt
     4321 atgctcgaaa tgttgatgca atggtatcgc cgccgtttta gcgacccgga agcgattgcc
     4381 ttgctggtta ttttagttgc cggatttggc attatctttt tctttagtgg cctgcttgct
     4441 ccgttgctgg tggctattgt gctggcctat ttgctggaat ggccaaccgt gcgcctgcaa
     4501 tctattggct gctcccgccg ctgggcgacg tcgattgtat tggtggtttt tgtcggtata
     4561 ttgctactga tggcgttcgt ggtactgcct atcgcctggc aacagggcat ctacttaatc
     4621 cgcgatatgc cggggatgct caataagctt tctgactttg ccgccacgtt gccgcgccgc
     4681 tatccggcgt taatggacgc gggcattatt gatgcaatgg ccgaaaatat gcgcagtcgg
     4741 atgctgacca tgggcgattc ggtggtgaaa atttccctcg cctcgctggt cggtttgctg
     4801 accattgccg tctatctggt gctggtgcca ttgatggtct tcttcctgct gaaagacaaa
     4861 gagcagatgc tgaacgccgt tcgtcgggtg ctgccgcgta accgtgggct ggcaggacag
     4921 gtgtggaagg agatgaatca acaaatcacc aactatatcc gcggcaaagt gctggagatg
     4981 atcgtggtag ggatcgccac ctggctgggg ttcttgctct tcgggctgaa ctattcgctg
     5041 ctgctggcgg tgctggtcgg cttctcggtt cttattccgt acattggcgc atttgtggtg
     5101 accattccgg tggttggcgt ggcgctattc cagtttggcg caggcacgga attctggagc
     5161 tgcttcgcgg tgtatctgat tattcaggcg ctggacggca acctgctagt accggtgttg
     5221 ttctccgaag cggttaacct gcatccgctg gtgattattt tatcggtggt gatcttcggt
     5281 ggtttgtggg gattctgggg cgtattcttc gccattccat tggcgacgct gatcaaagcc
     5341 gtgatccacg cctggcccga tgggcaaatc gcgcaagaat aatcttcagc ccgacgaact
     5401 gtttcgccgg gctgattttt caggattcct gacgtaaata aatagcccga taacacatac
     5461 tcaccagcac tgcaccgccg ataatattac ccagcatcac cgggagcaga ttggcggtaa
     5521 taaaatggct gaccgtcagt gccggaaaat tgtctgcgct actgtgcgcc agttgccaga
     5581 aggaggtagg ggcgaaatgg cgaatggcaa tggcgaacgg aatcacaaac aaattggcga
     5641 tacagtgctc aaagccactg gcgacaaaca gggtgatggg caaaatcatg gcgacgattt
     5701 tgtcgcataa cgaacgcccg caataactca tccacagcgc caggcaaacc atcagattgc
     5761 acataatgcc gaggctgaca gattcagtaa atgtatgatg cattttgccc tcggcgcagt
     5821 gtaaaaccgc gactccccac atcgcgtttt cactcatcac cagcccggaa aaccagatta
     5881 acaaactgaa acaggcaata cctgccagat taccgcaggc caccagaagt gcgttaatca
     5941 gccaagttcg ccaactaata acgcccctac tttttgccat taccgtcatt accgacgagg
     6001 tgaacaggtt ggtgccgcaa accgccagca aaataaagcc aagtgtaaag caaaggccgc
     6061 ccaccagatg ggttaatgcc tgtgaagacg gggcatcggc gataactgaa aggtaaaaag
     6121 taaatccgat cgccataaac acgccagcgg acatcgccat taaaaagacc gacactttac
     6181 tgcgggcaat tttatgggcg gcaatccgtt cagcgattgc cgcttttctg gcgctcaact
     6241 gcaagtcgaa agagagtttg tttctcatgc atactcctgc aaaagcagat tacaacacct
     6301 cgcgaaccga gattcccaga cgctgcattc gtgacagcag cgtggtgcgc ttcatcccta
     6361 atcgtgtcgc cgcgccacgg ggcccggcaa caatgccatt ggtttctcgc aatacctgaa
     6421 caatgcgctg acgttcttct tcgtcattct ctggcgtcgt cgggtgcagc aactgcgcca
     6481 ttgaactgcg aagcgctgaa tcttcattta gcgtcggcag taaacggctt tgtcggacat
     6541 taagatgtaa attcagactg ttaccgcgag tcagcagtac cgctcgctca atcacgtttt
     6601 ccagctcgcg cacgttgccc ggccagtccc acgacatcaa ctggcgtagc gcctcggtcg
     6661 ggatggcgtc aattgaacga ttcatatggc gcgccatttt ttgcgtgaaa tgctttgcta
     6721 aaagagggat atcttccgga cggtcgcgca gcggcggcaa ttccagtggg aagacattca
     6781 ggcgataaaa gagatcgctg cgaaactggc gatcttcaac catttgccac aaatcacggt
     6841 tggtggcggc aatgactctg acatttaccg ggatcgttct actcccgccg agacgctcaa
     6901 tctcccgttc ctgcaatacg cgcagcagtt taggctgaag ttctaacggc agatcgccaa
     6961 tttcatcgag aaacaacgtg ccgccatcgg caatttcaaa acgaccacga tgggtattaa
     7021 tcgcaccagt aaacgcccct ttgtcatgac cgaataactc actttccaga agactggcgg
     7081 ggatggcagc gcagttgatt ttgaccagcg gcttgtcgcg tcgcgggcta agttgatgga
     7141 tcgctctggc gatcacctct ttgccggttc cggtttcacc gcaaatcaac accgtactgt
     7201 cgctcttcgc cacaatatct acctgctgga gtaggtcttc cattgcctgg ctttgataga
     7261 taatgtcacc gatgcccaga ttcgaaagga gctgctcgct aagctggtgg ttttcttgct
     7321 gcaaactttc ctgcaaatcg gtcatgctac gccaggcatc ggcattgcca acggcaatag
     7381 cgatgcgatc ggctatgtgt tgtagtagct ggcagttttc ctcactaaag agagtggaag
     7441 aggtatgcgc cagcaacaat gcacccggtg tatggttgcc aaaggtaagc ggtataagga
     7501 gcgcagattc gcagccgtta gatgcaagta agagtaataa cgggtcgcgt tgccagagaa
     7561 acagatcgtc tgcacggtgc gtcaaggtcg gttgtcgggt ttgtagcgtt tgtgtcagca
     7621 atacactgtc gccaggcata ttgcgtggca gacacgcaca atgtgaggca gaaagatcgc
     7681 tgcaccacag gctaaacttc tcgttctttc gatgatcgcc cagtaccata ctgactgaag
     7741 ccagaccgaa aaaatgatgg atctcacgag cgacgtcagc gatcagatcg tcgagatcaa
     7801 gatgtgacag cactgcatta gtaatatcaa ccagtacccg atgatgatcg cgatctttct
     7861 ccagcaaacg cagcgccacc tgctgttctt catcattgag ccatgattgc agaaccgggg
     7921 tgattatccg cgccagccag ttaaacagtg tgcaatcctg gggagtaaaa cggctgccgt
     7981 gggtacgaat aaagcgtagc tccgccattc cactactgcc gagaaggacg gcgtgacgcg
     8041 aataatcgac catctcgccc tgatactctg ttgttgccgt cacatcgctg ccatcatcat
     8101 ggtagcggta atagaagttg cttttgagaa catgcagttc aataagttgt accgggctga
     8161 acggcggcgg catcgggatg aaggcgcgta ataaagacgc cttaccgtgt ttctgtgcta
     8221 accgctccgc gagcattccg tttaccgctt caattgttat tccttgtggc ggggcaaaca
     8281 tcgcctcgtc tgacatagcc atcaacagtc cttattaatg agaaatcctt ctcacgcaat
     8341 ttttctggcc atcagataga tggccggttc ctgcacaatt tcatccaaca gatgaatgaa
     8401 ttgaatgcta cgcgcctgcc acacagacgt cgcgtcatcc gccagtggcg cgaatgcccg
     8461 ggtcaacagg gcggtgtggc tggcgtcaat aacaatctca ccaaaagtca tcacccccag
     8521 catcttacgt cgggcttgct cctcttcgac ctgggcaagc caatcttcgt attccgtcag
     8581 tgggcagggg aaggcgacat tcagacaatc aatcacgcca acgtgatgac cgatagccag
     8641 cgagtaatac attacctgag agctgtgttc cggcatctcg tcgctactgg cgacaaactt
     8701 ttttcgcagc gtccagaaaa caatttcccc gcactcttca gtcatgtaac ccccggagta
     8761 cgcgaaagag ttgctgaacg atactattga gacgtggatc gtcggcatcg cgcaaccagg
     8821 tgttaacccg atttcctgta ggatcgtcgc ttaaatggcg caggagccga tcgcaaattt
     8881 ctcgcccctg acgatagccc gccagccgcc gcgcttctcg ctcaatggcg atacgctgtg
     8941 atggcgggat ctgcggccac aacggttgca tagtcacccc ggtgggatcg cgataatcca
     9001 ccgcgtgaat cttctgttgc agcaaaccga gcgccacggc gaaaccgtga atggtggcgg
     9061 ccggtgttgg cgggcagccg gggatccaaa catcaatggg gacaatggtg tcgctgccgc
     9121 cccagacgct gtagagatcg tggaaaatcc gccgccggca ccgcacgcgc cgtaggaaac
     9181 acagatttta tgatcggggg cagactcata cgcccgaagt gcaggcatac gcatcgcccg
     9241 ggtgactgcg cctgtaaata acaaaatatc ggcgtgacgc ggtgatgaaa taaccttaat
     9301 gccaaaacgt tctgcgtcga atactggtgt gatggcggca aaaatttcga tttcacaggc
     9361 gttgcagccg ccgcaatcga cgcgataaac gtaagccgag cgacggatat cctgtagcaa
     9421 atgctgcttc atcttttgcg tttgctcgtc cagcgtgatg ggctggctga catgttgtgt
     9481 aagcactgga ctcatagctg ctccttagcc accagcggta catctgtatc gtcgttgagc
     9541 agcgtcgcgc gttgtttgca ctccgggcag acgctcgctt gcgcccataa catttcgcgg
     9601 ttttgtggcg cattttgttg ctgtgccaac aattcagcag ccagtgcgac ggttttttgc
     9661 ggggcaaacg ggcgttcgca acggctgcaa cgttgtagat ggaacgtcgc gcgggtatag
     9721 agatcggctt tattggtgac ggtcagttca aagttattgg taagctggat ggctctggtc
     9781 gggcacactt cttcacaacg tccgcagtaa atacaacgcc tcagatagag ctgccaggtg
     9841 cgcgaatttt gctggtcgtc ggtctggata gtcagcgcat ttgccggaca agcacaggcg
     9901 caggcaccgc aggcaataca ttggctgggc atcaggtccg gtttcccgcg aaagcctggg
     9961 ctgacctcca gtggcgcgaa gggatatttc accgtcgcgg ttccggcgcg cataatagtt
    10021 ttcagtaact tcagcatctt ctgtccttac ttcagcggcg aacggttacg atcaataccg
    10081 tagcgttcga tctctttata cggcacggtt tttgactggc gcttgcgcac atctaccagc
    10141 gtcacacggt cggtacagga gtagcaggga tcaaggctac cgataatcag cggtgcgtca
    10201 gaaacggtat tgccgcgcag catgtaacgc aacaccggcc agttggcgta ggtggcggca
    10261 cggcagcgcc agcggaacaa tttttggtta tcaccgagca tgctccagtg cacgtcttca
    10321 ccgcgtggcg cttcaacaaa gcccagcgcg aatgcgtgag gtttatagct aaagccttcg
    10381 gtcagcagtg gggtatccgg catgttgtcg agggcaaatt ccagcattgc cagcgaatca
    10441 aacgtctctt tgacacggac catcacgcgg gagaaaacat cgccgccggt aaaggtaaac
    10501 agtgtttttg gaatattgcc gtagtcggcg taggggtgat caaagcgcaa atcacgggcg
    10561 atttgtcggt cgagaatgcc aatgccctga gtgcgttgtt ccatattcgg cgtagcaagc
    10621 agcatctcta ccagctccga cacgtcggcg cgcatctcgc gcaccagttt cagcgtttgc
    10681 agacgttgct ctttgagaat atcgcggcga acaccaccaa tcagattcag accgtaggtt
    10741 ttacgcgacc cgatcagcaa ttccgccatc gtcatcgact tttcccgcac gcggaaaaat
    10801 tgcataaagc cggtatcaaa accaacgaaa tggcaggaga ggccaaggtt aagcaaatga
    10861 ctgtgtagcc gttcgacttc cagcagaatc gagcgaatag tatgtgctcg ttgcggcacc
    10921 tcaatcccca gtgcattttc aaccgagttg gtataggcca cactgtgggc aaaaccgcaa
    10981 atcccacaca cgcggtccga taagaaggtc acttcgttgt agcccatccg cgtttctgcc
    11041 agtttctcca tgccgcgatg gacataaaac aggcggtaat cagcatcgac aatttgctcg
    11101 ccatccacaa acaagcggaa gtgacccagt tcatcggagg tgatatgcag cgggccgaca
    11161 gggatcacct gcgcatcgct gttgccctca ttgataaacg gatacgtttc ggaatcagtc
    11221 gtcggttcag ggcgcagtcg ataatccatc gcatctttgc gcagcggatg catatcttcc
    11281 ggccagtcat cgggcaacac caggcgacga tgatccggca ggccaaccgg aatcagcccg
    11341 tacatatcgc gaatttctcg ctcgccccag accgcggcag ggacgcgcgg tgtgactgac
    11401 ggaaactccc gactgtcggc atcgaccagt gccttcacca cgatccagca tttttcggcc
    11461 ccttccattg aaagggcata ataaaccgcg taatgaccgt taagtgtccg ctcgtcgttg
    11521 ccaaacagga ctggaagcca gccatcatgt tgataataaa gataatgtac aacgtcaggc
    11581 agcagattga ttttcaccgt aatggtgacc tgttcaggcg tttgtcgctc ttcatccagc
    11641 accgcgccgg ggaactgcgt tttcagggcg gcgagaatcg cttcgccacg atttgacgat
    11701 gaattaacgt tcactgtgtt tctccgaaac agatgcggtg cctgaaggca aaaaatcatg
    11761 ccaggtgcta cgttgtgcag gcaggtcgtg cgtccctgag aggactatag tggaagcgcc
    11821 cgccaggatc ctgatgacag gttgtggaat atgcgttccc atcgccagca tcatgaccag
    11881 cagaatcacc attggcgagg tggtcaacca gccgagatca ccccggttaa cggcctgcgg
    11941 cggtttcgcc attaacaccc gcgctgccat ccgtaccagg cccgccagca ccagcgttaa
    12001 cagcaataac agcaggacga taatcagcag gtgattacgt gccagtccgg cggtaatggt
    12061 cataaattcg ctaagaaaaa tgttgaaggg cggcatccct gccagcgcca gcgcaccgcc
    12121 gccaaacagc acggcggtaa atggcatgat tttgagcatc ccacagacga cgttgagatc
    12181 gcgcgtgccg tacttgagca gtacattgcc ggaaccgcag aacagcagcg tttttgccag
    12241 actgtggttt aaggtgtgca gcagcgcggc aaaaattccc agtggcccgc caatgcctag
    12301 cgccaccgcg accagtccca tgttctccac gctggagtac gccagcagac gcttaatgtc
    12361 ccgctgtacc agaatgaaaa atgccgccac ggcaaccgac aacatgccga agatgagcaa
    12421 caaccggttg gggaaatcgc tgccgatggc ctggcagata atgatgtaat agcgaattag
    12481 caccaacagc gcgcagttca gcaatacggc ggagagcagg gcgctgaccg gactcggtgc
    12541 ttcactgtga gcatccggca gccaggcgtg catgggaaat agcccggttt tggtgccaaa
    12601 gccaattagc acaaacacaa aggccaacag cattaatgtt gggtcaagca aggacgattg
    12661 cttaagaacc tcgctccaga atatcgccat ttctgcctgc ggcataacgc tggcgacgtt
    12721 ggcgtatacc agcacggtac cgaacagacc aaaagcgaca ccaacagtac aaataatgat
    12781 gtacttccat gctgcttcca gcgatgaacg ctgaccgtaa atgcctacca gaaacgccga
    12841 gcttaaggtg gtggcttcga tcgccgccca catcacaatc aggttattgc tggtaacaac
    12901 cagcagcatg gtaaacaaaa acagatggaa gaaaccgtag taatcgcaca gcgtaacggg
    12961 ggaaagctcg ccgtgtgcca cttcatgacg catgtagcca atcgagtaaa caccggtgag
    13021 aaagccaatc acaccaagaa tggcgaggaa caaaccgccc agaccatcaa tatgaagcca
    13081 cagtcccgca gcgaatattt ctcctgcatc agcggcagtt tggaccaccc agagtgccag
    13141 aatcagcagc agtgtgatcc ctaagctatg taatacagtc accgtacaag tcgcagagag
    13201 cccccgtttc cggcaggcaa aacagagcag cgaaaaaagc agcggcgtga gcaggagtaa
    13261 agcgaacatc acagaataac tcatctcatt atcccttcag cgcggtcaag ttgttcacgt
    13321 ccagcgtgcc gtgggtacgc catatttttc ttgccagtaa caccatcaca atgacggcga
    13381 agatggcgtc ggtagcgata cctatttcca ccagttccgg tgctcgccag gcaagaagcg
    13441 ccagcaccag atgggagccg ttttccatca ggcagtaacc aaaaatttgc cgcaggatat
    13501 tgcgctggct gacaatgcac agcaggccaa gcagaaaatg acctaacgct accgccagcg
    13561 ccggtttcag cccggtagcc atcggtagct tcacgggctg aacgacaaat gcgcaaagca
    13621 ggacaattaa cgccgcgagc agtgccatca ttgccggacc gaataacgct ttttccggga
    13681 tgttctgggg aatatttcgt gcagcgtaag tcatgattaa cggtaccagc agcactttgg
    13741 tgataaaggc gctggcggac cagatcagca gttgctctgc ggcgaacagg cacgagagag
    13801 tggcgaaaat agacaccagc accagcgact ggcaggcgta aaatccgcat gacaggcgat
    13861 agcttttgac gctaatcaca aacagcgatg tcagcatcat cagtcccgcc agattattta
    13921 cgatcataga accggtcatt catcatctcc ttactgcaac caggtcgcgg cgattacgct
    13981 ggaaatcagc gacatcacaa tcagtacaat caacaccagg cgcattgacc ctggcagcgg
    14041 tgcggcatcg gcgacggcct cgctcggttt gccaggcaca acgcgaccaa accagcgaat
    14101 aaaccaggcg aaactggcga ccgattcaat catcagcaga atcatggcgg gcagcaggat
    14161 ccagtactcc actgacaacg caaaaccggc agcaaacagc gggaatttac tgaagaagcc
    14221 gttgaacggc ggcacgccgg taatcgccag cgctgccacg cagaaaccca cgcctggcaa
    14281 cggcagggtg tgcaatacgc cacgcagacg cggcaacaag cgcgtgccgc agctgtaact
    14341 cagcgcacct gctacaagga aaaacaggct tttagcgaac gcgtggttga cgatgtaggc
    14401 gatgctgccc tccagcgcca gccgcgagcc gaagatggag agcgacaaac cgaagaacat
    14461 ccagccaagt tgagtgatgg tcgaccaggc cagcaaccgc ttcatatcct gctgtggcaa
    14521 atacatcaga aagccataaa gaatggtgac cagcgccatg accatgccaa cgccgccaat
    14581 cacatgcggg atattgccgc cgtcgataat ggcgcgggca aaaatgtaaa cgcccacttt
    14641 caccttcgat gcggcgtgga gataggcact gatcggtgtt ggcgcttcca ttgcatccgg
    14701 tagccacgct tgcatcggta gctgggccga tttcccccac gcggcaaaca ggatgccgcc
    14761 ataaaccaga taacgtgcgt cgccgtgtaa ctcgctcatc gcgctaagcg caaacgttcc
    14821 ggtctgcaaa aacagcgtgg cggcggcaag atacaacccc aacgagccga tatgagtgat
    14881 aagtaacgct tttagtgctg aacgctgtgc tttatcgctc tggtaataac tgatcaacgc
    14941 ccaggagcag ccgcccgtaa tttcaaaaaa caacaactga ccgagcagcg tcgaggagag
    15001 taccagtccc gccatcgcgc cgataaacaa cagcaaaaat gcgtaataac gattcgtgcc
    15061 gttatgcggg tgttcgcgat ttttatccgt cagataaccc gtggagtaga tcgtgaccag
    15121 caaaccgaga aacaccacca caaacagaat cagcgtactc acgcgatcaa tgaccaggcc
    15181 aaacaatgcc acatccccca cgttgaccaa tgtcaacgtg acggcaactt tatcggcctg
    15241 ataaaacgcg gagatcagtg acaacatgca cagcgtggtc agcgcggcga acaaaacccc
    15301 ccattcggcg gcccgacgtt gtggcgaaaa cgaaacgacc agtgcgccga taaaaggcag
    15361 caataacgtc gtcagagcaa gattttccat attccgtcag tgctccttac agaccggtta
    15421 accagaagac ccatgcaagc gcagcaaggc tgaagccaag ccaggtcaca tggtgaatga
    15481 gtaaaaaacg cccgcgtgcc agcgtgtttt ctgcgattga ggccagtaca aaaatcagca
    15541 aaaccttgag cagcgtaacg acaagtgaag tcagcaggca ggcgagagaa agttcttgcg
    15601 cgcgcccaaa ggacagaaac agggccacaa acagtgatgc catcacgacc tgtttcagcc
    15661 ccagccccca tttcgctagc gccagcccgg caccggaata ttcggtcagc gggccttcct
    15721 gtaattcctg ttctgcttca gcaacatcaa agggaatttt tcccatctca atgaagcagg
    15781 caaaaccaca ggccagtaac gccagtacgg tggttagcgg cgagttccag cccatcgcca
    15841 gcgtattgct gatcatctcg atatgcgtgg aacctgctat cagcgccaat accagcagtg
    15901 agagaataag cattggttcg accagaatgc cgagcgtcaa ctcgcgactg gcaccgactc
    15961 ccgcaaacgg acttccggta tccagcccgg aaagagcaaa gaaaaaacga aacagggcaa
    16021 gaagatagat aagggtgatc agatcgccgc cgcccgcaaa aggggaaacg gtaataaaca
    16081 gtggtaaggc catcgccagc accagcatgc tgctgattaa tacccacggc atcaggcgga
    16141 acatcagacc tgaagatgtc ggcgcaactt cctggcgttt aaacagtttg tggatatcgc
    16201 gataatcctg ccagatcccc ggcccgcggc gggagtgcat acgcgcgcgt atctgccggg
    16261 aaatacccgt aaaaagtggg gttagcatca gcagaatcac ggcctgtgct aacgcaaaaa
    16321 taaccagata tccgtcgcaa agagtttgtc tcatggtgat ttccttagac ggcaatagcg
    16381 attagcagca caaccagtgc ggcgaccaca tacaggcaat agagacgaaa gtcgccgctt
    16441 tgcagatgct gtatttcttt ggccagccgt tgggtggcgc tcacgatggg gcggatcacc
    16501 cgctcatccc agaagggttc tgtgctctga gccctggcgg tgacgtgcgc aaaaccttta
    16561 tttagcctca gcgtagggtc gagctgttgt cgtagacgaa ataacgcaga gaagaccaca
    16621 cgcagcggct gcatcacgcc attgcctgac ggggccatcg cattttccca gccgtagccg
    16681 catgcccagg catctcctgt gcgacgaaac gctgcgcggc gcgaacgaca ccacagccag
    16741 taaaggccag gcagcaaagg catcgccagt agtaacagca acagtaatga gggggtgacc
    16801 tgtgtatgaa acgtgccggg tacaagtgct attccgctgg cgacagtgac gggaggggta
    16861 ttggtaaacg catgggcaat atgcattatc ttcggtgcca gccaactggc actaagcgca
    16921 attaatacgc agagtgccgc gagcagtagc atggcgacga tcatcgtatt tggcacttcc
    16981 tgtgcctctt cagcgtgtgt actgcgcggc gcaccacaga aagtaatacc gtacattttt
    17041 acgaagcaca ttactgccag cccaccagtg actgccagca ttaccatagc aataggaccc
    17101 gcaagttgta gcgctacggc ttccacacga cttagtgaga acagcgactg ccaggtgtac
    17161 cattcgctga taaaaccatt cagcggagga atggctgata tcgcgaggca accaatcagg
    17221 catgctgcgg ctgtccacgg catccgtttc gccagtgccc ccattttttc catgtcgtgg
    17281 gtatgcaaac gcgaaataat cgcgcccgcg ccgagaaata gcagcccttt gaacagcgca
    17341 tggttgagca gatgaaacag tgcgccgagc agtccaacca cggtgagcag cgggtcgtgc
    17401 agtgacagac cgaccatcgc cacaccgact gccagcaaaa taatgccgac gttttcgacg
    17461 gtactccagg ccagcagccg tttgatatcc tgttccgcca gcgcatacag cacgcccagg
    17521 agcgcggaga ttgcgccgat cgccatcacc agaatgcccc accacagcgg caaacccgtt
    17581 tgcgccagca ggtccatcgc tactttcagg atgccgaaaa taccgatttt gaccattacg
    17641 ccagacatca acgccgaagc gtgcgatggt gcggcagggt gagcgcgcgg caaccagctg
    17701 tgcaacggca tcatcccggc tttcgcgcca aaaccgaaaa aggccagcag gaacaccgcc
    17761 gacgccagcc ccggagaaag tgaaagcgtg cggaaactgg caaaatcgag gctgccgctt
    17821 tcgcgcccca tcagcaagaa ggcgatcata atcagcaccg atccggcgtg ggcgataaaa
    17881 aagtagagca tgccagcgtt gatcgacgtt ttatcctgcc tggcaatgac cagaaaccag
    17941 gaagacagcg acatcatttc aaacagcacg atgaaccaaa aagcgttgtc catcaccagc
    18001 agggcaacca tcgatgcgat gaaaagattc ataaagaagc ccatcgccgc cgcgcctttg
    18061 ccctcgtatt cgcgcatata agtcaatgaa tagagcgaac aaaccaccac cagcaaggag
    18121 ataaccagca ccataaatgc cgagaggcta tccattcgca gggtaaaatc ggcaaagtca
    18181 aaaggggtgg caaaacgggc gactaaagtt acaccagcat gtaattgcgt aattccgctg
    18241 atgatgccaa tcacgccgcc caccagcgat gtgatgccgg aaagcttaat agccagtctg
    18301 tccagaccga gtaaaaacag cgaagccaga ctggcaaaca gatagaggat cagcgaccag
    18361 gttaataatt gcagggcatc cattagcgtt gctcctgagt gagggaaagg ggatcctccc
    18421 cgccattggc aaaccagctt gcggcaaggc gctgtttttc tttcatctgt cgttgcaggc
    18481 tatcgtcggt gatcagccgt aacgcctgat tcgggcaagc gcgaacgcag gccggacctt
    18541 ctggcaagaa atcacacagg tcgcatttca ccgcgacggt ctggacaccc ggttcccagc
    18601 gcagcaatgc atgttggggc ggcacgtttt cttcgccgtc tttgagtgag ccttcagcct
    18661 gaaaaacata ttgcgcaggg gcattcaccg gacggcttcc tgaagcgctg attgcgccaa
    18721 atgggcagac cacggcgcaa agcttgcagc caatacagag gctttcgttg agttggatcg
    18781 catcatccct ctgggagatg gcattaaccg ggcagacctg caggcaaggg gcttcctcac
    18841 agtgatgaca cacgacaggg gcagtgagtg ttgacgtctt cgccagggcc aggcgcgggt
    18901 gttgctgtaa accttgcgtt ttatgcacgt ccgaacaggc agcgagacag gtattacatc
    18961 ctgtacacca cagtggttcg gccaccacaa agcggttcat gtcggctcct tgaaaatgat
    19021 atgaaacatt gcatttcata ttgcataaag gatgccattc tgattaactc aatatattca
    19081 ataagttatg tattgactgg attctgaacg gtacaaaaat gatggtcatt aacgttcagc
    19141 aaaaaatgga ttcgacaccc gtgtcgcagg ggaaagcgag aatgtgatct tgatcacgag
    19201 aagtgaggta tgctggccgc aagcgcagcc agcacggaat ggagcaaagt aatcaggcgt
    19261 gttctttcag ccagttcagc acaacgtcgt ggtgattgct ggttttgaaa tcgtcaaaga
    19321 catgttcgat tttgccatca gcgtcaatca ggaagctgat gcgatgaatg ccatcgtagg
    19381 ttttgcccat gaaggacttt tcaccccaga cgccgaattg ttcgcacacc tggtggtcct
    19441 catcagacag gagcgtaaag ttaagcagct ctttttccgc aaaacgggag agtttttcgg
    19501 gtttatcggt gctgataccc agcacatcaa cgcccgcttt tttcaactca tccatgttat
    19561 cgcgtaagcc gcaggcctgt acggtacagc cgggggtcat ggctttcggg tagaaataaa
    19621 ccagaacacg ctgtccctgg aagtcggtca aattaacttg ttctccgtct tgatccggca
    19681 agctaaattt cggtgcgata tcaccggctt tcagtggatt cattacttaa ctccatcctg
    19741 ttcatcatgt tgggaataat tgacgacgtt aatactgcct tgtgcattga gttctgtaca
    19801 tagggctttg aacgcttgct caatatttgc tgcgtccgca gatgcggggc tgtgggcggt
    19861 tatctgaata tgcaactgcg cagcccgttc attttcagca ggttgcgtgc gcgacaccag
    19921 ctccgcaatg ttcatatgat gcgcgtcgaa aagtgctgtg aagcgttcaa ttaaatgcgg
    19981 ggagtctgcc acatcgacct gaacccagac agatgctggc attggcggac gcggacgcgc
    20041 cgtcgtgcgc ttcatcacga ttaaaagatc cagttcggca cctttcaacg gtaacgttga
    20101 ttcaatcaga gtgatggcat tccatgaacc ggaaagcagc atgataaacg tgaactcttc
    20161 tcccagcatc gccaggcgac tgtcttcaat attacagccg caactactga catgacgggt
    20221 gatggtgttc acaattccag ggcgatcggc acccaacgca gtgatcacca gataatgttg
    20281 cgatgacagt gtcaaactgg ttattccttt aaggggtgag ttgttcttaa ggaaagcata
    20341 aaaaaaacat gcatacaaca atcagaacgg ttctgtctgc ttgcttttaa tgccatacca
    20401 aacgtaccat tgagacactt gtttgcacag aggatggccc atgttcacgg gaagtattgt
    20461 cgcgattgtt actccgatgg atgaaaaagg taatgtctgt cgggctagct tgaaaaaact
    20521 gattgattat catgtcgcca gcggtacttc ggcgatcgtt tctgttggca ccactggcga
    20581 gtccgctacc ttaaatcatg acgaacatgc tgatgtggtg atgatgacgc tggatctggc
    20641 tgatgggcgc attccggtaa ttgccgggac cggcgctaac gctactgcgg aagccattag
    20701 cctgacgcag cgcttcaatg acagtggtat cgtcggctgc ctgacggtaa ccccttacta
    20761 caatcgtccg tcgcaagaag gtttgtatca gcatttcaaa gccatcgctg agcatactga
    20821 cctgccgcaa attctgtata atgtgccgtc ccgtactggc tgcgatctgc tcccggaaac
    20881 ggtgggccgt ctggcgaaag taaaaaatat tatcggaatc aaagaggcaa cagggaactt
    20941 aacgcgtgta aaccagatca aagagctggt ttcagatgat tttgttctgc tgagcggcga
    21001 tgatgcgagc gcgctggact tcatgcaatt gggcggtcat ggggttattt ccgttacggc
    21061 taacgtcgca gcgcgtgata tggcccagat gtgcaaactg gcagcagaag ggcattttgc
    21121 cgaggcacgc gttattaatc agcgtctgat gccattacac aacaaactat ttgtcgaacc
    21181 caatccaatc ccggtgaaat gggcatgtaa ggaactgggt cttgtggcga ccgatacgct
    21241 gcgcctgcca atgacaccaa tcaccgacag tggtcgtgag acggtcagag cggcgcttaa
    21301 gcatgccggt ttgctgtaaa gtttagggag atttgatggc ttactctgtt caaaagtcgc
    21361 gcctggcaaa ggttgcgggt gtttcgcttg ttttattact cgctgcctgt agttctgact
    21421 cacgctataa gcgtcaggtc agtggtgatg aagcctacct ggaagcggca ccgcttgcgg
    21481 agcttcatgc cccggctgga atgattttgc cggtgacctc cggtgattat gcaatcccgg
    21541 tgaccaacgg tagtggtgct gtcggtaagg cgctggacat tcgtccacca gcccagccgc
    21601 tggcactggt ttctggcgcg cgtacccagt tcacgggcga taccgcttca ttgctggtgg
    21661 aaaatggtcg tggcaatact ctgtggccgc aggtggttag cgtgctgcag gcgaaaaact
    21721 acaccatcac ccaacgtgat gatgctggtc agacactgac caccgattgg gtacaatgga
    21781 accgtctgga cgaagacgag cagtatcgtg gtcgttatca aatctctgtt aagccgcagg
    21841 gttatcagca ggcggttacg gttaaactgc tgaacctgga acaggcgggc aaaccggttg
    21901 cagacgcggc ttccatgcag cgttacagca cggagatgat gaacgttatt tccgccggtc
    21961 tggataaatc tgccactgac gccgcgaacg ctgcgcaaaa tcgtgcctcc accactatgg
    22021 acgtacaaag tgcagctgat gacaccggtt taccaatgct ggtcgtacgc gggccgttca
    22081 atgtggtttg gcaacgtctg ccagcggcgc tggaaaaagt gggcatgaaa gtgaccgaca
    22141 gcacccgttc gcagggcaac atggccgtaa cttataagcc gctgtctgac agcgactggc
    22201 aggaactggg cgcgagcgat ccaggcctgg catccggtga ctataaactg caggttggcg
    22261 atttagataa ccgcagcagc ctacagttca tcgatccgaa aggtcatact ctgactcaga
    22321 gtcagaacga cgcgctggta gctgtcttcc aggctgcgtt tagcaagtaa aaatacaggg
    22381 ctggaatcat ccggcccttt tttctgatat gatacgcaaa cgtgtgcgtc ggcaggaaaa
    22441 cgcgatttta gcggtaattc gcacgaaatt tgtttgtcgg acgtagttcg gataaggcgt
    22501 tcacgccgca tccgacaaaa catccggcac accagacagc aaaagatttt aaaacgttaa
    22561 ttcacaccca ggagtgataa agatgcaaaa gcaagctgag ttgtatcgtg gtaaagcgaa
    22621 aaccgtatac agcacggaaa acccggacct gttggtgctc gaattccgca atgatacgtc
    22681 agcaggggat ggcgcgcgca ttgagcagtt tgatcgcaaa ggtatggtga acaacaagtt
    22741 caattacttc attatgagca aactggctga agcgggtatc ccgactcaaa tggagcgtct
    22801 gctctccgat accgaatgtc tggtgaaaaa gctggatatg gtgccggttg agtgtgtcgt
    22861 gcgtaaccgt gctgctggct ctctggtgaa acgtcttgga atcgaagaag gtattgagct
    22921 gaacccgccg ctgttcgatc tgttcctgaa aaacgacgcc atgcacgatc cgatggtcaa
    22981 cgaatcttac tgcgaaacct ttggctgggt gagcaaagag aacctggcgc gtatgaaaga
    23041 gctgacctac aaagcgaacg acgtgctgaa aaaactgttc gatgatgctg gtctgattct
    23101 ggtcgacttc aagctggagt ttggcctgta caaaggtgaa gtggtactgg gcgatgagtt
    23161 ctccccggac ggcagccgcc tgtgggacaa agaaacactg gagaaaatgg acaaagaccg
    23221 tttccgccag agcctcggtg gcctgatcga agcctatgaa gccgtcgccc gccgcctggg
    23281 tgtacagctg gactgatttt tctggttgct tgtttcttta gccggagggt actccggcta
    23341 aatgtctttg atacccccgc tacaatactt ctcattatct ccctttgaca ggaaagtcat
    23401 accaggccaa tatgtatata cataacgtat atcccaaaaa gggggggcta tggaaactac
    23461 tgtattttta agtaatcgca gtcaggccgt cagattaccg aaagcggtcg ctctgccaga
    23521 agacgttaag aaagtagaaa ttattgcgat tggtcgtaca agaatcatca ctcccgccgg
    23581 tgagtcctgg gacagttggt ttgatggcga aaatgtgagt gcggatttta tggatatccg
    23641 tgatcaaccc gcaatgcagg aaagggaatc attttgatgc tgaaattcat gctggatacc
    23701 aatatctgta ttttcactat taagaataaa ccagtacatg tcagagagcg gtttaacctg
    23761 aattctggcc ggatgtgtat aagttctgta acattgatgg agcttatcta tggggcagaa
    23821 aaaagtcaga tgccagaacg caatttggcg gttattgaag gtttcgtatc tcgtttggta
    23881 gtgcttgatt atgatagtgc tgctgcgacg cataccgggc aaatcagggc cgagcttgcc
    23941 cggcagggaa ggcctgttgg accttttgac cagatgatag cgggacatgc tcgcagtcgc
    24001 ggtttgattg tggttaccaa caatacccga gagtttgagc gagtggcggg aatcagacta
    24061 gaagactgga gctaacattc tcttttactt ctaacattcg taaattatat aacgtggcat
    24121 gaagccttaa ttcctccgcc atttcatccc gctttcctgt ggtaatcttc ctctgaacca
    24181 cctacgatca tttctataat gaatatattg ttgaggtatc tatgcgttgg caagggcgac
    24241 gtgaaagtga caatgttgaa gacaggcgca acagctccgg tggtccgtct atgggcggtc
    24301 ccggttttcg cctgccaagc ggtaaaggcg ggttgatttt actgttagtc gtgctggttg
    24361 caggctacta tggtgttgat ttaaccgggt taatgactgg acagccggtt tcccaacaac
    24421 aatcaacgcg gtccattagc cctaatgaag acgaagctgc aaaattcacc tcggtgattc
    24481 tggcaaccac ggaagacacc tggggacaac agttcgagaa gatgggtaag acctatcagc
    24541 aaccgaagct ggtcatgtac cgtggaatga cgcgcaccgg ctgcggggct ggtcagtcca
    24601 ttatggggcc gttctattgc ccggcggatg gcacagttta tatcgatctc tccttctatg
    24661 atgacatgaa agacaaactt ggcgcggatg gcgattttgc ccaggggtac gttatcgccc
    24721 atgaagtcgg tcatcatgtg cagaaactgt taggcatcga gccgaaagtt cgtcaactgc
    24781 aacaaaacgc gacgcaggcg gaagtgaacc gcttatctgt gcgtatggaa ctccaggccg
    24841 actgttttgc cggtgtctgg gggcatagta tgcagcagca aggcgttctg gaaaccggcg
    24901 atctggaaga ggcgctgaac gcggcgcagg ccatcggcga tgaccgttta caacagcaaa
    24961 gtcaggggcg agtagtacca gatagtttca cacatggcac ttctcagcaa cgctacagct
    25021 ggtttaaacg tggtttcgac agcggcgatc cggcacaatg caatactttt ggtaaaagca
    25081 tttaacttcc ggggcaggga tggctgaact gactgcgctt cacacattaa cagcgcaaat
    25141 gaaacgtgaa gggatccgcc gcttgctggt gttgagcggg gaagagggtt ggtgttttga
    25201 tcatgcgctt aagttgcgtg atgccttacc tggcgactgg ctgtggattt cgccgcagcc
    25261 agatgctgaa aaccactgtt caccctcggc gctacaaact ttacttgggc gcgagttccg
    25321 gcatgcggta ttcgacgccc gccacggctt tgatgccgct gcctttgcag cacttagcgg
    25381 aacgttgaaa gcgggaagct ggctggtttt gttactccct gtatgggaag agtgggaaaa
    25441 ccaacctgat gccgactcgc tgcgctggag tgattgccct gaccctattg cgacgccgca
    25501 ttttgtccag catctcaaac gcgtacttac ggcggataac gacgctatcc tctggcggca
    25561 aaaccagccg ttctcgttgg cgcattttac tccccgtact gactggcacc ccgcgaccgg
    25621 cgcaccacaa ccagaacaac agcaactctt acagcagcta ctgaccatgc ctccgggcgt
    25681 ggcagcggta acggctgcgc gtgggcgcgg taagtcggcg ttggcagggc aactcatttc
    25741 tcgtattgcg ggcagtgcga ttgtcaccgc gcccgcaaaa gcggcaacgg atgtactggc
    25801 acaatttgcg ggcgagaagt ttcgctttat tgcaccggat gccttgttag ccagcgatga
    25861 gcaagccgac tggctggtgg tcgacgaagc cgcagccata cctgcgccgt tgttgcatca
    25921 actggtatcg cgttttcctc gaacgttgtt aaccactacg gtgcagggct acgaaggcac
    25981 cggacgtggt tttttgctga aattttgcgc tcgctttccg catttacacc gttttgaact
    26041 gcaacagccg atccgctggg cacagggatg cccgctggaa aaaatggtta gtgaggcact
    26101 ggtttttgac gatgaaaact tcacccatac accacaaggc aatatcgtca tttccgcatt
    26161 tgaacaaacg ttatggcgaa gcgagccaga aacgccgtta aaggtttatc agttattgtc
    26221 tggtgcgcac taccggactt cgccgctgga tttacgccgg atgatggatg caccagggca
    26281 acatttttta caggcggctg gcgaaaacga gattgccgga gcgctgtggc tggtggatga
    26341 ggggggatta tctcaacaac tcagtcaggc ggtatgggca ggttttcgtc gcccgcgggg
    26401 taatctggtg gcccagtcgc tggcggcgca cggtagcaat ccactggcag cgacattgcg
    26461 tggacggcgg gtcagccgga tagcagttca tccggcgcgt cagcgggaag gcacagggca
    26521 gcaacttatt gctggtgctt tgcaatatac gcgtgacctc gactatcttt cggtgagttt
    26581 tggttacacc ggggagttat ggcgtttctg gcaccgctgc ggttttgtgc tggtgcggat
    26641 gggtaatcat cgtgaagcca gcagcggttg ctatacggcg atggcgctgt taccgatgag
    26701 taatgcgggt aaacaactgg ctgaacgtga gcattaccgt ttacgtcgcg atgcgcaagc
    26761 tctcgcaaag tggaatggcg aaacgcttcc tgttgatcca ctaaacgatg ccgtcctttc
    26821 tgacgacgac tggcttgaac tggccggttt tgctttcgct catcgtccgc tattaacatc
    26881 gttaggttgc ttattgcgtc tgctacaaac cagtgaactg gcattaccgg cgctgcgtgg
    26941 gcgtttacag aaaaacgtca gcgacgcgca gttatgtacc acacttaaac tttcaggccg
    27001 caagatgtta ctggtccgtc agcgggaaga ggccgcgcag gcgctgttcg cacttaatga
    27061 tgttcgcact gagcgtctgc gcgatcgcat aacgcaatgg caattttttc actgactcct
    27121 tcaattttct cccatggtca ttgtgagtca gcggcgtaaa attaatgaca tcagttaagg
    27181 agttcgccat gaaacatgac cattttgttg ttcaaagccc ggataaacct gcgcaacagt
    27241 tgttactgct tttccacggt gtcggggata acccggtggc aatgggggaa ataggtagct
    27301 ggtttgcacc gctgtttcct gatgcgctgg tggtgagtgt cggtggcgcg gagccgagcg
    27361 gtaatccggc ggggcgtcag tggttttcag tgcagggtat tacggaagat aatcgccagg
    27421 cgcgtgtgga tgccatcatg ccgacgttta ttgaaacggt acgctactgg cagaaacaga
    27481 gcggagtagg ggctaacgcc acggcgctaa ttggtttctc acaaggcgcg attatggcgc
    27541 tggagagcat taaagccgaa ccgggccttg cttcgcgcgt cattgccttt aacggacgct
    27601 atgcgagcct gccggagacc gcgtctacag ccacgacaat tcacttgatt catggtggtg
    27661 aagatccggt tatcgatctg gcgcatgcag ttgcggcgca ggaagcgtta atcagtgccg
    27721 gtggtgacgt gacgctggat atcgtggagg atttaggcca tgcgatcgac aatcgcagta
    27781 tgcaatttgc cctggatcac ttgcgttata ccattccgaa acattatttc gacgaagcgc
    27841 taagtggcgg taagcctggc gatgatgatg tgattgaaat gatgtaaaag acagattact
    27901 tggcctgata agacgcgtga gcgtcgcatc aggcatcgtt caccattgcc ggatgcggcg
    27961 ttaacgcctt atccggccta cggttgagca ctcagcttac ttcttcggtt gatccttttt
    28021 cggccagtcg tcgtcatcgt cccacttatc gttgaaatca cgatgcgggg gaagctccgg
    28081 cttgttggcg agaaatttct tatggtcgac gcgcttgaga tctttaatca cgttcagcag
    28141 gacgcctacc aaaaagacaa tcaccagaat ccaccaatat ttagccagcc agtccatgct
    28201 tatttcctct tgcagaacca ctcatcaggc gacgagctgt tccatgatac gttgatacat
    28261 acgggcaagt agctgcaggt tggcagcgtt cacacattca ttaattttat gaatagtggc
    28321 attgaccggc ccgagttcca ccacctgcgc ccccatgcga gcaataaagc gcccgtcgga
    28381 cgttccgcct gtggtcagta gctgcggttt aatttcatta tagtgctcaa ccgcgttaac
    28441 gaccgcatcc accagtttac cgcgcgcggt caaaaatggc tgcccggaaa gccaccagtc
    28501 caccgtataa cgcagttgat gtttttcaag cagggcaagc acctgtgctt tgatcatctc
    28561 atcagtcagt tcggtgctga agcggaagtt aaactgcaca aacagttcac ccggaataac
    28621 gttgttactg cccgttcccg cctgaatatt ggcaatctgc atactggtcg ccgggaagaa
    28681 ttcattgccc tgatcccact caatagccac taattcatta aggaaaggtg ctgcgcgatg
    28741 taccggattg tcagccagat gcgggtaggc aacatgcccc tgaacgccat gaatggtgag
    28801 gttgcaggtt aacgatccgc gacgaccatt tttcaccaca tcacctacca cttcgatact
    28861 cgacggttcg ccgaccaggc agtaatcgag acgctcatta cgtgccatta acgcttcgac
    28921 gacttttacc gtaccgttgt gtgcactggc ttcttcatca gaggtgatca gaaatgccag
    28981 tcgccccgta tggttgggat gttgtgcgac aaagcgttct gccgctacca ccatcgccgc
    29041 cagcgagcct ttcatatctg ccgcaccgcg cccgaataac atgccatcac gaatggtggg
    29101 ttcaaacggc ggattgatcc aacgatcggc gtcgccaggc ggcaccacgt cggtatgccc
    29161 ggcaaaggct aacgtttcac cttgcccacg ccatgcccaa aaattctgcg tatcggcaaa
    29221 gtccatgcgt tcaacggtaa aaccgatcgc ctgcaaacgt tcaatcaaca aagcctggca
    29281 tcctgcgtca tcaggactca gggaagggcg gcgaataagc tgttgtgtca gctcaataac
    29341 cgggcacgac atagactaca cctcatggaa aaattgctga taactggaat cactgaaacc
    29401 cagcagcata ggcttaccag gcgcgcagag caatggacgt ttgataattg caggcatttc
    29461 agtcattaat gccgccgcag aggccgcatc ggtgatttta ttgcgggtgg tttcgtccag
    29521 tttacgccag gttgtaccac gggtgttgag taacgcttcc cagcctaatt cgttgataaa
    29581 accgttcaat aattcgctgt ccagcccatc gacgcggtaa tcatgaaaac gatagtcgat
    29641 gttattggct tccagccaac ggcgagcctt tttaatggtg tcacaatttt tgatgccgta
    29701 aagtgtaacc ataatgaatc ctttttcgat ttatgagaat aaatactcat ttaaggggga
    29761 aaacattttg cctgcaaatg attatcagaa aatataacat tatcgaagta ttcaaattaa
    29821 caactattaa ataatattaa acatttttaa cgtaaacatt aagaatattc atcttgttat
    29881 ctgtctgtat tttaaccaga attaaatcag tattagatgc cgtatgaata tctcgaaatg
    29941 ttgctgtgta tcacatacaa attatcaccg caggaccata gtatttacat aaggtttgtc
    30001 ccccggaggt agtcatcgca gcaatcgagt aaattagctt caccacggta tattgtttcc
    30061 gtagaatacc cataattata agagaggttg ttatgattga acgtgaactg gggaactgga
    30121 aagactttat cgaagttatg cttcgtaagt aattcccgag acaaagtata aacagcaaga
    30181 acccgctgag caggttctta atcggaaagt tgaaaaaggc gacacattgg catgtcgcct
    30241 tttttattgc ttattccggg cgcggcttca gcgggaagcg gcggcgcacc agcacaaaga
    30301 acagcggcac gaagtaaata gccagaatag tagctgaaat catcccgccc attacgccag
    30361 tacccaccgc atgctgacca ccggaaccgg cacccgtgct ggttgccatt ggcaatacgc
    30421 cgaagataaa tgccagcgag gtcatcagaa tcgggcgtaa acgctgacgg caggcgtgga
    30481 gcgtcgcttc aaacaggtcg tggccttttt ggttcatctc gttagcaaac tcgacgatca
    30541 ggatggcgtt tttcgccgat aaaccaatga ccgttaacag gcccacctgg aagtaaacgt
    30601 cgttttccag cccgcgcatc caggttgcca gcagcgcgcc gataaccccc agcggcacga
    30661 ccagcattac cgagaacggc accgaccagc tttcatacaa cgccgccaga cacaggaata
    30721 ccaccagcaa ggaaatggcg tacagcgccg gagcctgcgc gccggaaagt cgctcctgat
    30781 acgacatcgc cgtccactcc agaccaaagc cgtttggcaa ctgcttcact aacgattcca
    30841 taatatccat cgccgtaccg gtactgaccc ccggtgcggc ttccccaaca atctcaaccg
    30901 cagaataacc gttatagcgt tccagacgcg gcgagcctgt ttcccagcgt gaggtcgcga
    30961 aagcagagaa gggcaccatg ccgccatctt tatttcggac ataccagaga ttaatgtcat
    31021 ctggcagcat gcgatacggc gcagctgcct gcacatagac tttcttcacg cgaccgcgat
    31081 ccataaagtc attcacatag ctcgaacccc aggcggtttg cagtgtgtcg ttaatatcgt
    31141 cgatagcaac gcccagcgcc tgagctttac gctggtcgat atcaatctgc aactgcggac
    31201 tgtcgtcgag gccgttatgg cgcacacggg ttagctccgg gttttccgcc gccagcgcca
    31261 gcaactgatt acgtgctgcc atcagcgcat cgtgacccgc tccagcgtgg tcctgcaact
    31321 ccatatcaaa acctgcagaa ctaccaagac cgctaattgc tggcgggctg ctggcgataa
    31381 cgcgagcttc tttaatttgg ttaaacgcct tcgttgcacg ctcgataatg gcaaacgagg
    31441 tgccggtctt actgtcgcgt tcgctccagt ctttcaggcg gataaacatt cgcgccacgt
    31501 tttgcccgtt acccccgggg ccagaaccaa cagtggcaaa caccgacatg atgttgtctt
    31561 tttcatgggt gaagtaatat ttctcgattt gctcaacgac tttcagggtc tgttgttggg
    31621 ttgaaccgct gggcaattgt accgaggtag taaacatgcc acggtcttcc agcggtaaga
    31681 acgacgtcgg caaacgcagg aacaggaaca ccatgccgcc aagcagcagg acataaatca
    31741 caatccagcg caggctacgg tggagaattt tcgccacccc tttttcgtag cgttcggcgt
    31801 tgcggttaaa catctggtta aaccaggcaa aaaagccttt ttgcccgtga tgctcacctt
    31861 ttttcagtgg cttaagtagt gtggcacaca gagccggagt gaggatcatc gctaccagta
    31921 ctgacagcac catcgccgca acaatggtaa tagagaactg gcgatagatg gcaccggtgg
    31981 tgccgccgaa gaaggccatt ggtacaaata ccgccgaaag aaccatcgca atcccgacca
    32041 gtgccccctg gatctgcccc atcgatttac gtgtggcttc gcgaggagtg agtccttcct
    32101 cactcataat acgttcgacg ttttccacca ccacgattgc gtcatccacc agcagaccga
    32161 tcgccagtac catcgcgaac atggttaagg tgttgacgct gtaaccgaag gcgtaaagta
    32221 cggagaaggt tcccatcaac accaccggca cggcgatagt gggtatcagc gtggcgcgga
    32281 agttttgcag gaacagatac ataacgagga aaaccagggc gatagcttcc agcagcgttt
    32341 tcaccacgtc ttcaatggag gctttaacaa acgaggtggt ttcatacgcc accttgtatt
    32401 ccagtccgtg cgggaagtac tgcgccagct cgtcgagacg attgagcacc agctccgctg
    32461 tcgccatttc gtttgcgccg gaggccagtt ttacccccag cccggaggct ggcttaccat
    32521 tgaagcggct aagataatcg tatttctccg cccccatttc gacggtggcg acatcgccca
    32581 gccttacctc tgagccgtct tgattcaccc gcaaggtgat atcgcggaac tgttctggtg
    32641 tttgcagcag tgactgggcg ttaatggtgg cgttgagcgc ctgcttatcg acggaaggtg
    32701 taccaccaag ttgcccaacc gcaatctgcg cgttctgtga ctcaatggca tcagtgacat
    32761 ctttagccgt catctggaaa ctgttgagtt tcgccgggtc cagccagata cgcatggaat
    32821 attgcgaacc ataggcatcg atatccccga cgccattcac gcggcttaac gggtcctgaa
    32881 tattactggc aacataatcg gcaatatcct gtttatccat cgaaccatcg gtagagacga
    32941 aagcaatggt cagaatattg gtatcgccgg ttttacgcac cgtcacgccc tgattttgca
    33001 ccgcctgcgg taactttcgc atggctgatt gcagctggtt ttgtacttgc tgcacggctt
    33061 cgtccggatc ggtgcctgct ttaaaactta aagtgacaga tgcctgaccg gtgccactgc
    33121 tctgagatga catatacatg agattatcga ggccggtcat attttgctcg ataacctggg
    33181 tcacggtgtt ttccagcgtc tgggccgatg cgccgggata gttagcggtc actcgcacat
    33241 tcggtggcgc gagatcgggg tattgttcaa cgggcaatga aaaaatcgcc agggtccctg
    33301 tcagacacaa caggattgcc agcacccagg caaaaatggg gcgatcaata aagaaattcg
    33361 ccattaaaag aggacctcgt gtttctgcgt atcgttgtag gcttgacttg cttcactgta
    33421 gcggcaaggt acgagccaat cgtggagaaa aaaaggagtt aatgtaaatt atcatgccct
    33481 tttatactga accttaagtg caagtattct ttggtcagta ggaggcatta catcaactga
    33541 ctttcctcac cctccgcaga gcgaaagcta atactcacta acgtgccgcc gccggaaggt
    33601 tgtgaaaaag tcagcgtccc acccagccgt tccgcgcgtt cgcgcatgat attcagacca
    33661 taatgacctt cgggttcttt cggttcaccg ataccaatcc cgttatcacg gatataaacc
    33721 gtgtgattgc cgtccggcgc ggtgacgcaa ctgacggcga tttcgctggc gttggcgtgc
    33781 ttcatcgcat tcagcaccgc ttcgcgaata atttgcaaca aatgcacctg catttgcgca
    33841 tccagcgcca gggttggcag acggcagtcg agggtcagtt tggcgctggt ttgattttgt
    33901 aacgtatcca gcatttccct caacgcggag gggagatccg cctgctgcag cgtcaggcgg
    33961 aaagtagtca acagctcgcg taactgccga taagcatcat tcaacgcctg ggaaaaatcg
    34021 gccatgatac tttgtgcggt ggcgttatct tccggtatcg aacgcttcag taacgtcaac
    34081 tggatacgta agtaagaaag tacctgagcc agcgagtcgt gcaattcgcg ggcgatggtt
    34141 gcacgttctt ccatcaacaa taactgctga aaatgcttct gcgcctgatt aaagtacaaa
    34201 ccgcgtccca gcatcgatga aacactgtta agcagcggtt ctgatgatga aacgtgacta
    34261 ttttgccagt gcagttcgcc gtaaaccgtc tcttgcattg tcaccggtaa aatctgcatc
    34321 ggcaattccg ggtttggttg cccttcgcta atccgccagt tttcaccgac atttaactcc
    34381 agatattcag ccgcttcatt gtcgcgaaca atctgcaaaa tatggcggaa acaatgcaca
    34441 tcaatctggc tggtgttcag cgcctgggaa cattgataca acacctccag acgacgcttg
    34501 gcctcgtgga gatcgcgggt cttttcttct actgacgcct ccagtgaaag gtacaattta
    34561 tgcagctcgc tcgacatctg gttaaaggtt tttgcaagca gaccaagctc attcggcaga
    34621 ctggtatcca gcggcggcga gtcgaactgc ccgtgttcaa tacgttgact ggcggtaacc
    34681 agctgattca gcggggcaac cacctgatgg cgtatacggc gcaaagtaaa aaagaccagc
    34741 gtgaaaatac cgataccgcc agccagggaa atcgccacca ccagcagcat tttgcgttca
    34801 gcgtagtgct gtaaagccag cacgaacagg tctatctgat taacataatt attaatattg
    34861 gcctgatacc acggcaaatc gcccttgctg agccgattat tcatctccag ccagttggca
    34921 ttcagatgcg catagcgagt ttttactgct tctggcacat accagacgtt gagattggtt
    34981 aataccggtg aatgcagtgc ctgctgaaac aactggcgat gtgcattgag ttgtggactg
    35041 ccactttgca agtcgtagcc caggcggtaa ctctgcatac gtagcgatcc ggcaatattg
    35101 atagcctcag cgtcgcgcaa actgctcgcc agagttagca gagcgatacc cgtggaaaga
    35161 atcgacagca gcacaatgta aaaaaaggcc cgggccagac tggccgagac gggtcgttta
    35221 acaatcacac gcgtcttctc cacaaaaaat cattaatgtt ccagttagct gaaacacaat
    35281 ttagtcagtg atgggattat ttgatctgcc acagtttata gcgaaatagt tacttattct
    35341 gggcacatga acattgccga ttcccctgta agtcgggtaa taacaacact catataaaga
    35401 ataaggtttt tacaaccaaa aaagaaggtc gttatgaatc gttttattat ggccaacagt
    35461 cagcaatgtc tgggttgtca tgcttgtgaa atcgcctgtg tcatggctca caatgatgag
    35521 caacatgtcc tgagccaaca ccattttcat ccccgaatta cggttatcaa acatcaacag
    35581 caacgtagtg cagtgacctg tcaccattgt gaagatgcgc cctgcgcccg tagctgccct
    35641 aatggcgcaa tcagccacgt tgatgacagc attcaggtca atcagcaaaa gtgtattggc
    35701 tgtaaatcct gcgtggtggc ctgtcctttt ggtacgatgc aaatcgtcct gacacccgtc
    35761 gcggcaggaa aagtaaaagc cacggcgcat aaatgcgacc tttgtgcggg gcgcgaaaac
    35821 ggtcctgcct gtgttgagaa ttgcccggcg gacgcgctgc aactggtcac tgacgtcgca
    35881 ctctccggca tggcgaaacc ccgccgcttg cgcaccgcgc gtcaggaaca tcaaccgtgg
    35941 catgccagta ccgcggcgca agaaatgccg gtaatgagta aagtcgaaca aatgcaggca
    36001 acgcccgcgc gtggcgagcc ggataaactg gcgattgaag cgcgcaaaac cggttttgat
    36061 gaaatttatc tgccatttcg cgccgaccag gcacaacggg aagcctcgcg ctgccttaag
    36121 tgcggcgagc acagcgtttg tgaatggacc tgcccgctgc ataaccatat tccgcagtgg
    36181 attgaactgg tgaaagccgg aaacatcgac gccgccgtcg agctttctca ccagaccaac
    36241 accctgccgg aaattaccgg acgcgtttgt ccgcaagacc gtttgtgtga aggtgcctgt
    36301 actattcgcg atgagcacgg cgcggtaact atcggcaata ttgaacgcta tatttcagat
    36361 caggcgttgg cgaaaggttg gcgtcctgac ttaagtcatg tcaccaaagt ggacaagcgg
    36421 gtggcgatta tcggggcagg tccggcaggg ctggcctgtg cggatgttct gacccgcaat
    36481 ggcgtggcgg tgacggtgta cgatcgccat ccagaaatcg gtggcttgct cactttcggc
    36541 attccttctt tcaaactgga taaatccctg ctggcacgcc gtcgggaaat cttcagcgcg
    36601 atggggattc acttcgaact caattgtgaa gtgggtaaag atgtctcttt ggattcgctt
    36661 ttggaacaat acgacgcggt cttcgttggc gtaggcactt accgttccat gaacgcgggt
    36721 ttacccaatg aagatgcgcc gggcgtttat gacgcgctgc cgttcctcat tgccaacact
    36781 aaacaggtga tggggctcga agagctaccg gaagagccgt ttatcaatac cgccggactt
    36841 aacgtcgtgg tactgggcgg cggcgacacc gcgatggact gtgtgcgtac tgcgctgcgc
    36901 cacggcgcga gtaacgtcac ctgcgcttat cgtcgtgatg aagctaacat gccaggctcg
    36961 aagaaagagg tgaggaacgc ccgcgaagag ggggccaact tcgaatttaa cgtccagccg
    37021 gtggcgcttg agctgaatga acaaggtcac gtctgcggga ttcgtttcct gcgcacgcgt
    37081 cttggcgagc cggatgccca ggggcgtcgg cgacctgtac cgattgaggg cagtgaattt
    37141 gtcatgccag ccgatgcggt gattatggcg tttggcttca atccacacgg gatgccgtgg
    37201 ctggagtcgc acggtgtaac ggtagacaaa tggggccgca tcatcgcgga tgtggaaagc
    37261 cagtaccgtt accagaccac caatccgaaa atcttcgccg gtggcgacgc cgtgcgtggt
    37321 gcggatctgg tggttaccgc aatggcagaa ggacgtcatg cggcacaggg gattattgac
    37381 tggctggggg taaaatcagt caaatctcac tgatagcctg cgcagacaaa cccgacttca
    37441 cagcgtaaga taattgttca tttcgcgctg tggagtcggt atgacgcaac aaatcaccct
    37501 cattaaagac aaaattctct ccgataacta tttcaccctg cacaacatta cctacgatct
    37561 cacgcgcaaa gatggcgaag ttatccgcca taaacgtgaa gtttacgatc gcggtaatgg
    37621 tgcgacgatc ctcctgtaca acgcgaagaa aaagagcgtg gttctgattc gtcagttccg
    37681 tgtcgctacc tgggttaatg gcaatgaaag cgggcagctg attgaaacct gcgccgggct
    37741 gctggataac gacgaaccgg aagtgtgcat tcgtaaagaa gcgattgaag agacgggcta
    37801 tgaagtgggc gaagtgcgca aattatttga actgtatatg tcgcctggcg gtgtgactga
    37861 gctaatccac ttttttatcg ccgaatacag tgacaatcag cgcgctaacg ccggtggcgg
    37921 tgttgaagat gaagatattg aagtgctcga gctgccgttc agccaggcgc tggagatgat
    37981 caaaaccggc gagatacgtg acggtaagac ggtgttattg cttaactatt tgcaaacgtc
    38041 acatttaatg gactgaaaaa taacaataat atttcgttgt ttattattgg ctcaatccga
    38101 caaatctgat tgagccgcgc tacctgcgca acgaagatac gacttgtgct gtttgtttga
    38161 acttctgggg tcgtaccgtc catgcgctat cgcattttcc ttctcttttt tttcgctttg
    38221 ttgccgacgt ctttggtgtg ggcggcacca gcgcaacggg cgttttccga ctggcaggtc
    38281 acctgtaata accaaaactt ctgcgtggcg cgcaatacgg gcgatcataa tggactggtg
    38341 atgaccctga gccgcagcgc cggggcgcat accgatgccg ttttacgtat tgagcgcggc
    38401 gggttgaagt cgccggatgc gtctgaagga gagatagcgc cacggctgct gttagatggt
    38461 gagccgttgg cgttaagtgg tgacaagtgg cggatttcac catggttatt agtaaccgat
    38521 gatacggcaa ccatcaccgc gtttttgcag gtgattcagg aagggaaggc gatcacctta
    38581 cgcgacggca atcagaccat ttctctgagt ggcttaaaag cggcgttgtt gtttattgat
    38641 gctcagcaaa agcgcgttgg cagtgaaacc gcgtggatca agaaagggga cgaaccgccg
    38701 ctcagcgtac cgcccgcgcc tgcgctgaaa gaggtcgcgg tggttaaccc aacgccgacg
    38761 ccactctcac tcgaagaacg caacgatttg ctggattatg gaaactggcg gatgaatggt
    38821 ctgcgctgct cgcttgatcc gttacgtcgt gaggtgaatg tcactgcgct gactgatgat
    38881 aaagcgctga tgatgattag ctgtgaggca ggggcctata acaccattga tttggcatgg
    38941 attgtgtcgc gtaaaaagcc actagcctcg cgcccggttc ggttgcgttt gccattcaac
    39001 aacggtcagg agacgaatga actggaactg atgaacgcaa catttgatga gaaatcgcgt
    39061 gaactggtga ccttagcgaa agggcgcgga ttaagcgatt gtggcattca ggcgcgctgg
    39121 cgctttgacg gtcagcgttt tcgcctggtg cgttatgccg cagaacccac ctgcgataac
    39181 tggcatgggc cagacgcctg gcccacgttg tggatcaccc gttaaacaaa attgcgggga
    39241 catgtaggcc ggataagacg ctgtaggccg gataagacgc gtcagcgtcg catccggcaa
    39301 tcagcatccg gcgatcacca tcaggcacct ttcactccca gcaccttatg cgcttttgcc
    39361 acaatattct cggcggtaaa gccaaagaac gggaacagct tatccgccgg agcagattcc
    39421 ccgtaacccg tcatcccgac aattacccct ttcagaccaa catacttgta ccagtaatcg
    39481 gcaatacctg cttccaccgc cacgcgagcc gcaacgttag aaggcaacac cgactcccga
    39541 tattcctcat cctgggcgtc gaaaatatcg gtcgagggca gggaaactac gcgtacattg
    39601 cgaccttctc ctgctaattt ctctgccgct tgcagggtaa tttccatctc tgaaccggtg
    39661 gcaatcagaa taatatctgg cttaccgccg ctgtctttca gcacataacc gccacgagca
    39721 atctctttaa cctgatccgg cgtacgttcc acctgggcca gattctgcct tgagaggatc
    39781 agtgctgtcg gcccgttgtg gcgctcaacc gccagcttcc agcccaccgc cgcttccacc
    39841 tgatcgcacg gtcgccaggt gctgaaattc ggcgttaagc gcagactggc cagttgctca
    39901 acggcctggt gcgtcggacc atcttcccct aagccaatag aatcgtgggt ataaaccata
    39961 atctgccgtg ctttcatcag tgccgccatc cgcgcggcat tacgggcgta ttcaacaaac
    40021 atcaggaacg tcgcggtata cggcacaaag ccgccgtggt gcgcaatgcc gttggcgata
    40081 gcggtcatgc caaattcacg caccccgtag tgaatgtagt tgcccgccgg atcttccttc
    40141 agcgacacag agcctttcca gatggtgagg ttgctgggag ccagatccgc tgaacctccg
    40201 agcagctccg gtagcatcgg tccgtaagcg ttaagcgtat tttgcgaagc cttacgggtg
    40261 gcgattttcg ccggattcgc ctgcaactca ttgatatatt tctgagtcgt tttctcccag
    40321 tccttcggta aaccaccgct catccgacgg gtaaactctt ctgccagttg cggatgagcc
    40381 tttttatagg cggcaaactt ctcattccag ctctgctgcg ctttttcgcc tttttcgcgg
    40441 gcatcccagg cgtgataaat ctttttaggg atctcaaatg gcggatggtg ccagcccagt
    40501 ttttgccgtg ccagcgccac ttcttcttcc cccagtggtg cgccgtgcgc ctcttcctta
    40561 cctgctttat tcggcgaacc aaagccaatc accgtacggc agataatcag cgacggctta
    40621 tctttcatgc tttgcgcttc aaggatcgct tccttcaccg cctgcggatc gtgaccgtcg
    40681 atttcatgga tcacatgcca gtgataggct tcaaaacgtt ttgccgtatc gtcggtaaac
    40741 cagccttctg tttcaccgtc gatggaaata ccgttgtgat cgtaaaaacc aatcagcttg
    40801 cccagtccca gcgtgcctgc cagcgaacag acttcgtggg aaataccttc catcaggcag
    40861 ccgtcgccca taaacacata ggtgaagtga tcgacgatct catggtctgg ctggttaaac
    40921 tgcgccgcca gtgtacgctc tgctatcgcc agcccgacgg cgttcgccaa accttgtcca
    40981 agcgggccgg tggtggtttc aacgcctggc gtatagccaa tctccgggtg gcctggggtt
    41041 ttcgaatgca actgacggaa gttcttcagt tcttccagcg gcaggtcgta accggtcaga
    41101 tgtagcaaac tgtagagcag catcgacgcg tgaccgttgg aaagaataaa gcggtcgcga
    41161 tcataccagg ttgggtcggt aggattatgt ttaagaaaat cgttccacag cacttcggca
    41221 atatcagcca tgcccatcgg cgcgccggga tgaccagaat tggctttttg taccgcatcc
    41281 atactgagtg cgcgaatcgc attggcaagg tcttttcggg acatataaca ctccgtggct
    41341 ggtttatagt ttggcggcaa gaagatcttc cagtttgcgt tgatcaacag cgaacagacg
    41401 aatgccttca gacagttttt ctaccgccat cgcatcctga ttgtgctccc aacggaactc
    41461 cgcttcgctc atgggtgctg ggcgtgggaa cgtctgagaa ggcgggatta atttacgtac
    41521 cactggcgaa actttttcct gcagctcctt cagcaaattc ggtgcgatag tcagtcgatc
    41581 gcagccggtt aaggcgagga tttgttcggt gcgacggaag ctcgcgccca tcacaatggt
    41641 ttcatagtgg tgttgcttat agtagtcgta gatattgcgc accgatttaa cgcccggatc
    41701 ttcttccacc acatacgggt ccatcggctt gcgtgcctga taccagtcat aaatacgccc
    41761 gacaaacggc gaaatcagaa aaacgcccgc ttctgcacag gcccgcgcct gggcaaaaga
    41821 aaacagcagc gtcaggttgc agttaatacc ttctttttcc agctcttcgg ccgcgcgaat
    41881 tccttcccag gtcgaagcca gtttgatcaa aatgcgtgat ttctcaacgc cttgttgctg
    41941 atacaagtcc accagatggc gcgccttttc aatactcttt tctttatcaa aagagaggcg
    42001 tgcatcaact tctgttgaca cgcgaccggg tacgattttg agaatttcag caccgaaatt
    42061 gactgccagt ttgtcacacg ctgcgaccac ctgttgttcc tgggtcttgc catttttttt
    42121 accccaggcg atagcatcgt ctattaaatg ctcatattgt gataatccgg cagctttgag
    42181 taacagcgaa ggattggtgg tggcatcctg gggatgataa tggcgaatgg actcaatatc
    42241 gccgctgtct gccacgacag tggtgaactg tttgatgccg tctaactcgt tcataggaaa
    42301 tactccttga aaagtaaagt gttagatgag tgcgttaatt cacacttctg agaaatttcg
    42361 ctaaacgcat caaaaaagca tagcagacag gcatggtatt gctggattaa gcaggtaaca
    42421 tcagtgttat aggattatta ccaaaacatt atattaatgg tgagagtttg gacttgctca
    42481 aagtctgtag actccggcag ggtaataatg tgcgccacgt tgtgggcagg ggctgttgcc
    42541 cacacacttt atttgtgaac gttacgtgaa aggaacaacc aaatggatga ccagttaaaa
    42601 caaagtgcac ttgatttcca tgaatttccg gttccaggga aaatccaggt ttctccaacc
    42661 aagcctctgg caacgcagcg cgatctggcg ctggcctact caccaggcgt tgccgcacct
    42721 tgtcttgaaa tcgaaaaaga cccgttaaaa gcctacaaat ataccgcgcg cggtaacctg
    42781 gtggcggtga tctctaacgg tacggcggtg ctggggttag gcaacattgg cgcgctggct
    42841 ggcaaaccgg tgatggaagg caagggcgtt ctgtttaaga aattcgccgg gattgatgta
    42901 tttgacattg aagttgatga actcgacccg gacaaattta ttgaagttgt cgctgcgctc
    42961 gaaccgacct tcggcggaat caacctcgaa gacattaaag cgccagaatg tttctatatt
    43021 gaacagaaac tgcgcgagcg gatgaatatt ccggtattcc acgacgatca gcacggcacg
    43081 gcaatcatca gcactgccgc catcctcaac ggcttgcgcg tggtggagaa aaacatctcc
    43141 gacgtgcgga tggtggtttc cggcgcgggt gccgcagcaa tcgcctgtat gaacctgctg
    43201 gtagcgctgg gtctgcaaaa acataacatc gtggtttgcg attcaaaagg cgttatctat
    43261 caggggcgtg agccaaacat ggcggaaacc aaagccgcat atgcggtggt ggatgacggc
    43321 aaacgtaccc tcgatgatgt gattgaaggc gcggatattt tccttggatg ttccggcccg
    43381 aaagtgctga cccaggaaat ggtgaagaaa atggctcgtg cgccaatgat cctggcgctg
    43441 gcgaacccgg aaccggaaat tctgccgccg ctggcgaaag aagtgcgtcc ggatgccatc
    43501 atttgcaccg gtcgttctga ctatccaaac caggtcaaca acgtcctttg cttcccgttt
    43561 atcttccgtg gcgcgctgga tgttggcgca acggctatca atgaagagat gaaactggcg
    43621 gcggtacgcg cgattgcgga actggcccac gccgaacaga gcgaagtggt ggcttcagcg
    43681 tatggcgatc aggatctgag ctttggtccg gaatacatca ttccaaaacc gtttgatccg
    43741 cgcttgatcg ttaagatcgc tcctgcggtc gctaaagccg cgatggagtc gggcgtggcg
    43801 acgcgtccga ttgctgattt cgacgtctac atcgacaagc tgactgagtt cgtttacaaa
    43861 accaacctgt ttatgaagcc gattttctcc caggctcgca aagcgccgaa gcgcgttgtt
    43921 ctgccagaag gggaagaggc gcgcgttctg catgccactc aggaactggt aacgctggga
    43981 ctggcgaaac cgatccttat cggtcgtccg aacgtgatcg aaatgcgcat tcagaaactg
    44041 ggcttgcaga tcaaagcggg cgttgatttt gagatcgtca ataacgaatc cgatccgcgc
    44101 tttaaagagt actggaccga atacttccag atcatgaagc gtcgcggtgt cactcaggaa
    44161 caggcgcagc gggcgctgat cagtaacccg acagtgatcg gcgcgatcat ggttcagcgt
    44221 ggggaagccg atgcaatgat ctgcggtacg gtgggcgatt atcatgaaca ttttagcgtg
    44281 gtgaaaaatg tctttggtta tcgcgatggc gttcacaccg caggtgcaat gaacgcgctg
    44341 ctgctgccga gtggtaacac ctttattgcc gatacctacg tcaatgacga accagacgca
    44401 gaagagctgg cggagatcac cttgatggcg gcagaaactg tccgtcgttt tggtattgag
    44461 ccgcgcgttg ctctgttgtc gcactccaac tttggttctt ctgactgccc gtcgtcgagc
    44521 aaaatgcgtc aggcgctgga actggtcagg gatcgtgcgc cagatctgat gattgatggt
    44581 gaaatgcacg gcgatgcagc gctggtggaa gcgattcgca acgaccgtat gccggacagc
    44641 cctttgaaag gttctgccaa tattctggtg atgccgaaca tggaagctgc ccgcattagt
    44701 tacaacttac tgcgcgtttc cagctcggaa ggtgtgactg tcggtccggt gctgatgggc
    44761 gttgcgaaac cggttcacgt gttaacgccg atcgcatcgg tgcgtcgtat cgtcaacatg
    44821 gtggcgctgg ccgtggttga agcgcaaacc caaccgctgt aattcttttt taactctcac
    44881 gcttaccctg aatattcagg gtaagcggtt tggctgcaat atattagtaa agcttattac
    44941 tgagtttgcg aataataaaa aaagcagtct atataatatc tcgatattat tttatttata
    45001 ttcatgcgtt gcatatgaaa gtttatgcac cacagcgaat atctctccat tcttagtgat
    45061 ctacctcacc ttttaaacgc gcttgccgaa ttttgttatt tactctgacg aaaaattgtc
    45121 acgatacacg aaagtttttc acaggcggcg actcatggat aaagaacgca tcattcagga
    45181 atttgtgccc ggcaaacagg tcacactggc gcatctcatc gcgcatcccg gtgaggaact
    45241 ggcgaagaaa atcggcgttc cggatgcggg cgcaattggc attatgacgc taactcccgg
    45301 cgaaacggcg atgatcgccg gtgatttagc gctgaaagcc gctgatgtgc atatcggttt
    45361 tctcgaccgc ttcagcggtg cattggtgat ttatggctcg gtgggcgcag tagaagaagc
    45421 gttatcacaa accgtcagcg gtttaggtcg cttattaaat tatacgcttt gtgaaatgac
    45481 caaaagttaa tacgaggcat tcatgaaacg aattgcgttt gtgggtaccg tcggggcagg
    45541 aaaaacaacg ttatttaatg cgttgcaggg gaattatacc ctcgccagaa aaacacaggc
    45601 cgtggaattt aatgataagg gcgatattga tacgccgggt gaatatttta gccatccccg
    45661 ctggtatcac gccttaatta ccacgctaca ggatgtagac atgctgatct atgtccatgg
    45721 cgcaaatgac ccggaaagtc ggttgcctgc cgggttgctg gatattggcg tcagcaaacg
    45781 gcaaatcgcc gtcatcagta aaacggacat gccagatgct gacgtcgccg caacgcgaaa
    45841 gttattgctt gagacgggtt tcgaagagcc gattttcgag ctaaacagcc acgacccgca
    45901 aagcgtgcag caactggtgg attatctggc atctctcacc aaacaggagg aagcaggtga
    45961 aaaaactcat cacagcgaat gatattcgtg aagcacacgc gcgcggcgaa caggcaatgt
    46021 ccgtggttct gcgcgccagc attattaccc cggaagcccg tgaagtggcg gatctactgg
    46081 gttttaccat caccgaatgt gatgaatcga tcccggtaac ggcgtccgtg cccgccagcg
    46141 tgccagccga caaaaccgaa agccagcgca tccgcgaaac catcattgcc cagctgccgg
    46201 aaggccagtt taccgaaagc ctggtcgcac agctgatgga aaaagtgatg aaggaaaaac
    46261 agtcgctgga gcagggcgca atgcagccga gcttcaaatc ggtgaccggc aaaggcggca
    46321 tcaaagtgat tgatggcagc agcgtcaagt ttggtcgttt cgacggcgca gagccgcatt
    46381 gcgtgggctt gactgatctt gtgaccggag acgacggcag cagcatggcc gctggtttta
    46441 tgcagtggga gaacgcattc ttcccgtgga ccctgaacta cgacgaaatc gacatggtgc
    46501 tggaagggga gctgcatgtt cgccacgaag gcgaaaccat gatcgccaaa gcgggtgacg
    46561 tgatgtttat cccgaaaggc tccagtatcg aatttggtac gacatccagc gtgaaattcc
    46621 tgtatgtcgc ctggccggct aactggcaat ccctatgaaa gatttcatca ccgaagcatg
    46681 gctaagagcg aaccatacgc tcagcgaagg cgcagagatc catctccctg cggacagtcg
    46741 cctgacgccc tctgcccggg agttactgga aagccgccat ctgcgcatca agtttattga
    46801 cgagcagggc cgcctgtttg ttgacgatga acagcagcag ccgcagcccg ttcatgggct
    46861 gaccagtagc gatgaacatc cgcaggcgtg ctgcgaactg tgccgccagc cggtggcaaa
    46921 aaagccggat acgctgactc acctgtcggc ggaaaaaatg gtcgccaaaa gcgatccgcg
    46981 tctgggtttt cgcgcggttc tcgacagcac cattgcgctg gcagtgtggc tgcaaattga
    47041 actggcggaa ccgtggcagc cgtggctggc ggatatccgt tcgcgtcttg gcaacattat
    47101 gcgcgccgat gcgctgggtg aaccgctggg ctgccaggcg attgtcgggc tttctgacga
    47161 agatctgcat cgactttctc accagccgct gcgctatctc gaccacgatc atctggtgcc
    47221 ggaagccagc cacgggcgcg atgcggcgtt gctcaatctg ctacgtacca aagtgcggga
    47281 aaccgaaacc gtagcggcgc aggtgtttat cacccgcagc tttgaagtat tacgcccgga
    47341 cattttgcag gcgctgaacc gcctttccag cacggtctac gtgatgatga tcctgagcgt
    47401 aacgaagcag ccgctaacgg tgaagcagat ccaacaacga ctgggggaaa cgcaatgatt
    47461 attgaacgtt gtcgtgaact ggcgttgcga gcgcccgcca gagtggtttt tccggatgcg
    47521 ttagaccaac gtgtgctgaa agccgcgcaa tatttacatc aacaaggtct ggcaacgccc
    47581 attctggtcg ccaatccgtt tgaacttcgt cagtttgcgc tcagtcacgg cgtggcgatg
    47641 gacgggctac aggtgattga tccgcatggc aacctcgcaa tgcgggaaga atttgctcat
    47701 cgctggctgg cccgcgcggg cgaaaaaacg ccgccggatg cgctggaaaa acttaccgac
    47761 cctctgatgt tcgccgcagc aatggtcagc gccggtaaag cggatgtctg tatcgcgggc
    47821 aacctctctt ccacggcaaa tgtgctgcgt gccggattac gcattatcgg cttgcagcca
    47881 ggctgtaaaa cgctctcttc cattttcctg atgttgccac agtacagcgg cccggcgttg
    47941 ggctttgccg attgcagcgt ggtgccacag ccgacggcgg cgcagctggc ggatatcgcg
    48001 cttgccagtg ccgaaacctg gcgcgccatc accggagaag agccgcgcgt ggcgatgctg
    48061 tcgttttcca gcaatggtag cgcccgtcac ccctgtgttg ctaacgtcca gcaggcgaca
    48121 gaaatcgtcc gtgagcgcgc accaaagctg gtggtggatg gcgagttaca gtttgacgcc
    48181 gccttcgtgc cggaagtggc ggcgcaaaaa gcgcctgcca gcccgctaca gggcaaggcc
    48241 aatgtgatgg tttttccgtc gctggaagcc ggaaatattg gttacaaaat cgcacaacga
    48301 ctcggcggat atcgtgccgt cgggccactg atacaaggac ttgccgcgcc gatgcacgat
    48361 ctctctcgtg gttgtagcgt gcaggaaatt atcgagctgg cgctggtggc agctgtgccg
    48421 cgtcagacag aagtgaaccg cgaaagcagt ttacaaacac tggttgaatg aacggtcccg
    48481 ttctggaccc tgataaagag gaaagcacaa tggaagcatt aggaatgatc gaaacccggg
    48541 gcctggttgc actgattgag gcttctgacg cgatggtgaa agctgcgcgc gtcaagctgg
    48601 ttggtgttaa gcagattggc ggcggcctgt gtactgccat ggttcgtggt gatgttgcag
    48661 cctgtaaagc cgcgaccgat gcaggtgccg cagcggcaca gcgtatcggc gagctggtat
    48721 ctgtacacgt tatcccgcgt ccgcacggcg acctggaaga agtcttcccg atcggcctga
    48781 aaggcgatag cagcaacctg taatcaccct gtgtgacgtc gattcgggcg tcacgctctc
    48841 tcctttttca ttttaccttc tgcggagggt agggggagct tttacctgaa atacgccacg
    48901 gaggcgggta tgaaactggc agtcgtcact ggacaaattg tttgtaccgt acgccatcac
    48961 ggactggcgc atgacaaatt gctgatggtg gaaatgattg atccacaagg taatcctgac
    49021 gggcaatgcg ccgtcgccat cgacaatatt ggcgcgggaa ccggggagtg ggtgttactg
    49081 gtgagtggca gttccgcccg ccaggcgcat aaaagcgaaa cgtcaccggt cgatctgtgc
    49141 gtgattggca ttgtcgatga ggtggtgtct ggcggtcagg taattttcca caaataaggc
    49201 agaacatcat gaatcaacag gatattgaac aggtggtgaa agcggtactg ctgaaaatgc
    49261 aaagcagtga cacgccgtcc gccgccgttc atgagatggg cgttttcgcg tccctggatg
    49321 acgccgttgc ggcagccaaa gtcgcccagc aagggttaaa aagcgtggca atgcgccagt
    49381 tagccattgc tgccattcgt gaagcaggcg aaaaacacgc cagagattta gcggaacttg
    49441 ccgtcagtga aaccggcatg gggcgcgttg aagataaatt tgcaaaaaac gtcgctcagg
    49501 cgcgcggcac accaggcgtt gagtgcctct ctccgcaagt gctgactggc gacaacggcc
    49561 tgaccctaat tgaaaacgca ccctggggcg tggtggcttc ggtgacgcct tccactaacc
    49621 cggcggcaac cgtaattaac aacgccatca gcctgattgc cgcgggcaac agcgtcattt
    49681 ttgccccgca tccggcggcg aaaaaagtct cccagcgggc gattacgctg ctcaaccagg
    49741 cgattgttgc cgcaggtggg ccggaaaact tactggttac tgtggcaaat ccggatatcg
    49801 aaaccgcgca acgcttgttc aagtttccgg gtatcggcct gctggtggta accggcggcg
    49861 aagcggtagt agaagcggcg cgtaaacaca ccaataaacg tctgattgcc gcaggcgctg
    49921 gcaacccgcc ggtagtggtg gatgaaaccg ccgacctcgc ccgtgccgct cagtccatcg
    49981 tcaaaggcgc ttctttcgat aacaacatca tttgtgccga cgaaaaggta ctgattgttg
    50041 ttgatagcgt agccgatgaa ctgatgcgtc tgatggaagg ccagcacgcg gtgaaactga
    50101 ccgcagaaca ggcgcagcag ctgcaaccgg tgttgctgaa aaatatcgac gagcgcggaa
    50161 aaggcaccgt cagccgtgac tgggttggtc gcgatgcggc gaaaatcgcg gcggcaatcg
    50221 gcctgaacgt gccgcaagaa acgcgtttgc tgtttgtgga aaccactgca gaacatccgt
    50281 ttgccgtgac cgaactgatg atgccggtgc tgcccgtcgt gcgcgtcgcc aacgtggcgg
    50341 atgccattgc gctggcggtg aaactggagg gcggttgcca ccacacggcg gcaatgcact
    50401 cgcgcaatat cgaaaacatg aaccagatgg cgaacgccat tgataccagc attttcgtta
    50461 agaacggacc gtgcattgcc gggctggggc tgggcgggga aggctggacc accatgacca
    50521 tcaccacgcc aaccggtgaa ggggtaacca gcgcgcggac gtttgtccgt ctgcgtcgct
    50581 gtgtattagt cgatgcgttt cgcattgttt aaggagtaac cgatggcgca cgacgaacaa
    50641 tggctcaccc cacgtctgca aacggcggca acgctgtgta accagacgcc tgccgcgacg
    50701 gaatctccgc tgtggctggg ggtggatctg ggcacctgcg atgtggtgtc gatggttgtt
    50761 gaccgcgacg gtcagccggt ggcggtatgc ctcgactggg ccgatgtggt ccgcgacggc
    50821 atcgtctggg atttcttcgg tgcggtcacc attgttcgtc gccatctcga cactcttgag
    50881 cagcaattcg gtcttcgttt tagccatgcg gcgacttcat ttcctccagg tacggacccg
    50941 cgtatttcca tcaacgtgct ggaatctgcc gggctggaag tgagccatgt gctggatgag
    51001 ccaaccgcag tcgcggatct gctgcaactg gacaacgccg gtgtagtgga tatcggcggc
    51061 ggcactaccg gcattgccat cgtgaaaaag ggcaaggtga cgtactcggc ggatgaagcc
    51121 accggcggac atcacatctc tctgactctc gccggaaatc gtcgtatttc gctggaagag
    51181 gcagagcagt acaagcgcgg tcatggtgac gagatttggc ccgcggtgaa accggtgtac
    51241 gaaaaaatgg cggacattgt cgcccggcat attgaagggc agggcattac tgatttatgg
    51301 ctggcgggcg gctcttgtat gcaaccgggc gtggcggaac tgtttcgcaa acaattcccg
    51361 gcgttacagg tgcatttacc gcagcacagt ttgtttatga ccccgctggc gatcgccagt
    51421 agtgggcgag agaaagcgga ggggctatat gcaaaatgaa ttgcagaccg cgctctttca
    51481 ggcgttcgat accctgaatc tgcaacgggt aaaaacattt agcgttccac cggtgacgct
    51541 ttgcggtccg ggctcggtga gcagttgcgg acagcaagcg caaacgcgtg ggctgaaaca
    51601 tctgttcgtg atggcagaca gctttttgca tcaggcaggg atgaccgccg ggctgacgcg
    51661 tagcctggcc gttaaaggta tcgccatgac gctctggcca tgtccggtgg gcgaaccgtg
    51721 catcaccgac gtgtgtgcag ccgtggcgca gttgcgtgag tcaggctgtg atggggtgat
    51781 cgcatttggc ggcggctcgg tgctggatgc ggcgaaagcc gtggcgttgc tggtgacgaa
    51841 cccggatagc acgctggcag agatgtcaga aaccagcgtt ctgcaatcgc gcctgccgtt
    51901 gattgccatt cccactaccg ccggaaccgg ctctgaaacc accaacgtaa cggtgattat
    51961 cgatgcggtg agcgggcgta agcaggtgtt agcccatgcc tcgctgatgc cggatgtggc
    52021 gattctcgac gccgcattga ccgaaggtgt gccgtcgcat gtcacggcga tgaccggcat
    52081 tgatgcgtta acccatgcca ttgaagcata cagcgccctg aacgctacac cgtttaccga
    52141 cagtctggcg attggtgcca ttgcgatgat tggcaaatcg ctgccgaaag cggtgggcta
    52201 cggtcatgac cttgccgcgc gcgagagcat gttactggct tcatgtatgg cgggaatggc
    52261 gttttccagt gcggggcttg ggttgtgcca cgcgatggcg catcagccag gggcggcgct
    52321 gcatattcca cacggtctgg cgaacgccat gttgctgcca acggtgatgg aatttaaccg
    52381 gatggtttgt cgcgaacggt ttagtcagat tggtcgggca ctgcgaacta aaaaatccga
    52441 cgatcgtgac gctattaacg cggtaagtga gctgattgcg gaagttggga ttggtaaacg
    52501 actgggcgat gttggcgcga catctgcgca ttacggtgca tgggcgcagg ccgcgcagga
    52561 agatatttgt ctgcgcagca acccgcgtac cgccagcctg gagcagattg tcggcctgta
    52621 cgcagcggcg caataaatgc cggatgcgac gcttgccgcg tctgatccgg ccttcgggat
    52681 tgcacatgta gggcggataa ggcgtttacg ccgcatccgc caataaataa caactaacag
    52741 ggagtaaagg cgatgggaat taacgaaatc atcatgtaca tcatgatgtt ctttatgctg
    52801 atagctgccg tagacaggat cctgtcgcag ttcggcggtt ctgctcgttt cctcggtaag
    52861 ttcggtaaaa gtatcgaagg atcaggcggt cagttcgaag aaggctttat ggcaatgggc
    52921 gcactgggcc tggcgatggt cggtatgacc gcgctggcac cggtactggc tcacgtactc
    52981 gggccggtaa ttattccggt ttacgaaatg ctcggcgcta acccatcgat gttcgccgga
    53041 acactgctgg cgtgcgatat gggcggcttc ttcctcgcca aagagctggc gggcggcgac
    53101 gtagcagcgt ggctatactc tgggttaatt ctcgggtcga tgatggggcc aacgattgtg
    53161 ttttccattc cggtggcgct cggcattatc gaaccttctg accgtcgtta tctggcgctc
    53221 ggcgtgctgg cgggcattgt gaccattccg attggctgta ttgctggtgg tctggttgct
    53281 atgtactccg gtgtgcagat caacggtcag ccggtggaat tcactttcgc cctgatcctg
    53341 atgaacatga tcccggtgat cattgtcgcg attctggtgg cgctggggct gaaattcatc
    53401 ccggaaaaaa tgatcaacgg cttccagatc ttcgccaaat tcctcgtggc attgatcacc
    53461 ctcggtcttg ccgctgcggt agtgaaattc ctccttggct gggaactgat ccccggactg
    53521 gatcctatct ttatggcccc tggcgataaa cccggtgaag tgatgcgcgc cattgaagtt
    53581 atcggttcta tctcctgcgt gctgttaggg gcgtatccaa tggtgctgct gctgactcgc
    53641 tggtttgaaa aaccgctgat gagcgtcggt aaagtgctga atatgaacaa catcgcggca
    53701 gccggcatgg tggcaacgct tgccaacaac atcccgatgt tcggcatgat gaagcagatg
    53761 gatacccgcg gcaaagtcat caactgcgcc ttcgccgttt ccgctgcttt cgccctgggc
    53821 gaccacttag gcttcgccgc tgccaacatg aacgccatga tcttcccgat gattgtcggc
    53881 aagttgatcg gcggcgtaac ggcgattggc gtggcgatga tgctggtgcc aaaagaagac
    53941 gcgaccgcga ctaaaaccga agcggaggca caatcgtgaa cactcgccag ctattgagcg
    54001 tcggtatcga tatcggcacc accaccaccc aggtgatttt ctcccacctg gagctggtta
    54061 accgtgcggc ggtgtcgcag gtgccgcgct acgaattcat taaacgcgaa attagctggc
    54121 aaagtccggt gttctttacc cctgtcgata aacagggcgg tttaaaagaa gcggaactga
    54181 aaaccttaat actcgagcaa tatcaggctg cgggtattgc gccggaaagc gttgattctg
    54241 gtgccatcat catcaccggt gaaagcgcga aaacccgcaa tgctcgcccg gcggtgatgg
    54301 cgctctctca atcgctgggg gattttgtcg ttgccagcgc cgggccgcac ctcgaatccg
    54361 tgatcgccgg tcacggagct ggggcgcaaa ccctttctga acaacggctg tgtcgggtac
    54421 tgaatatcga catcggcggt ggcaccgcga actacgccct gttcgatgcc ggaaaaatca
    54481 gcggcactgc ctgtctcaac gtcggtggtc gcctgatgga aaccgacagc caggggcgcg
    54541 tggtttacgc tcataaaccg gggcagatga ttgtggatga gtgcttcggt gcaggcactg
    54601 acgcccgttc gctgaccggc gcgcagctgg tgcaggttac ccggcggatg gcggcgctga
    54661 ttgtcgaagt gattgacgga acgctttcgc cgctcgcgca ggcattgatg caaaccggtt
    54721 tgctgcccgc aggtgttacg cccgaaatca ttacgctttc tggaggcgtg ggcgaatgtt
    54781 atcgccacca gcccgccgac ccgttctgtt ttgccgatat tggcccactt ctggcaacgg
    54841 cgctgcatga ccatccgcgc ctgcgtgaga tgaatgtgca gtttccggcg caaaccgtac
    54901 gcgccacggt gattggcgcg ggtgcacata ccctttcgct ctctggcagc acaatctggc
    54961 tggagggcgt acaactgccg ctgcgcaatt tgccggtggc gatcccgatt gatgaaacgg
    55021 atctggtgag tgcctggcaa caggcgctgc ttcagctgga tcttgatccc aaaactgacg
    55081 cgtacgtgct ggcgcttccc gcctcgctgc ctgtgcgtta cgccgcggta ctgacggtca
    55141 tcaacgcgct ggtcgatttc gtcgcgcgtt ttccgaatcc gcatcccctg ctggtggtgg
    55201 ccgggcagga ctttggtaaa gctctgggca tgttgttgcg cccacagcta caacaactcc
    55261 cgttggcagt cattgacgaa gtgattgtcc gcgcggggga ctatatcgac attggtacgc
    55321 ctctttttgg cggatcggtt gtgccggtga cggtgaaatc actcgcattt ccttcctgag
    55381 ggaacgactt atgaaactaa agaccacatt gttcggcaat gtatatcagt ttaaggatgt
    55441 aaaagaggtg ctggctaaag ccaacgaact gcgttcgggg gatgtgctgg cgggcgttgc
    55501 agcggcaagc tcacaggagc gcgtggcggc aaagcaggtg ttgtcggaaa tgaccgtagc
    55561 ggacatccgc aataatccgg tgattgccta tgaagatgac tgcgtgacgc ggctgattca
    55621 ggacgatgtt aacgaaacgg cctacaacca gattaaaaac tggagcatca gcgaactgcg
    55681 tgagtatgtg ctgagcgatg aaaccagcgt ggacgacatt gcctttaccc gcaaagggct
    55741 gacctcggaa gtggtcgcgg cggtagcgaa gatttgctcc aacgcggacc tgatctacgg
    55801 cgcgaagaaa atgccggtaa tcaaaaaggc caataccacc attggtattc cgggcacctt
    55861 cagcgcccgt ttgcagccga acgatacccg tgacgacgtg caaagtatcg ctgcgcaaat
    55921 ctatgaaggg ctttcctttg gggtgggcga tgcggtgatc ggcgttaacc cggtaactga
    55981 cgacgtggaa aacttaagcc gcgtgttgga caccatttac ggcgtgatcg acaaattcaa
    56041 catcccaact cagggctgcg tactggcgca cgtcaccacc cagatcgaag cgattcgtcg
    56101 cggcgcgcct ggcggactga ttttccagag tatctgtggc agcgaaaaag ggctgaaaga
    56161 gtttggcgtg gaactggcga tgctcgacga agcgcgcgca gtgggtgcag agttcaaccg
    56221 tatcgccggg gaaaactgcc tctacttcga aaccggacaa ggctctgcgc tatccgctgg
    56281 cgctaacttc ggcgctgacc aggtgacgat ggaagcacgt aactatgggc tggcgcgtca
    56341 ttacgatccg tttatcgtca acaccgtggt cggctttatt gggccggagt atctctacaa
    56401 cgaccgccag attatccgtg ctggcttaga agatcacttt atgggcaagc tgagcggcat
    56461 ctctatgggc tgtgactgct gttacaccaa ccacgctgac gctgaccaga acctcaacga
    56521 aaacctgatg atcctgctcg ccaccgcagg ctgcaactac atcatgggga tgccgctggg
    56581 tgatgacatc atgctcaact accagaccac cgcattccac gataccgcca ctgtgcgtca
    56641 gttactcaac ctgcgtccgt caccggagtt tgaacgctgg ctggaaagca tgggcattat
    56701 ggcaaacggt cgcctgacca aacgggcggg cgatccgtca ctgttcttct gatgacgcgg
    56761 agataacaca tcatggatca aaaacagatt gaagaaattg tacgcagcgt gatggcgtca
    56821 atgggacaaa cggcccccgc gccgtcagaa gcaaagtgcg ccaccaccaa ctgtgcggca
    56881 ccggtgacct cggaaagctg cgcgctggat ttaggttctg cggaagcaaa agcgtggatt
    56941 ggtgttgaaa acccgcatcg cgcagacgta ttaacagaac tgcgtcgcag caccgtggcc
    57001 cgcgtttgta ccggtcgtgc cggtccgcgt ccgcgtacgc aggcgttgct gcgtttcctg
    57061 gccgatcact ctcgttcgaa agataccgta ctgaaagaag tgccggaaga gtgggtgaaa
    57121 gcgcagggct tgctggaagt acgctccgaa attagcgaca aaaatctcta cctgactcgc
    57181 ccggatatgg gccgccgcct gtgtgcagaa gctgttgaag cgctgaaagc acagtgtgtt
    57241 gctaatccgg acgtacaggt cgttatttct gatggcctgt caactgatgc catcaccgtg
    57301 aactacgaag agatcctgcc gccgctgatg gcgggcctga aacaggccgg gctgaaagtc
    57361 ggcacgccat tctttgttcg ttatggtcgc gtgaagattg aagatcagat cggtgagatc
    57421 ctcggcgcga aagtggtgat cctgctggtg ggggaacgtc cggggttagg gcagtcagaa
    57481 agcctctcct gctacgccgt ttactcgccg cgtatggcga ccaccgttga ggccgatcgc
    57541 acctgtatct ctaacattca ccagggcggc acgccgccgg ttgaagccgc agccgtcatt
    57601 gtggatttgg cgaaacgtat gctggagcag aaagcatccg gcatcaacat gacccgataa
    57661 ggaggcatca tgccagcttt agatttgatt cgaccgtcgg taaccgccat gcgggtgatt
    57721 gcctccgtta acgctgaatt tgcgcgtgaa ctaaaattgc cgccgcatat tcgtagcctc
    57781 gggctgattt ctgccgattc tgacgacgtc acctacattg cggctgacga agcgaccaaa
    57841 caagcgatgg tcgaagtggt atatggtcgc tcgctgtatg cgggagcggc acacggcccg
    57901 tcaccgactg ccggtgaagt gctgattatg cttggtgggc caaacccggc ggaagtgcgt
    57961 gccggtctgg atgcgatggt tgcacatatt gaaaatggcg cagctttcca gtgggccaat
    58021 gacgcagaaa acaccgcctt cctggcacat gtagtttcgc gtaccggttc ttatctctca
    58081 tcaaccgccg ggatcacgct tggcgatccg atggcgtatc tggtggcacc gccgctggaa
    58141 gcgacctacg gcattgatgc agcgttgaaa tctgccgacg tacagctggt gacctatgtc
    58201 ccgccaccgt ctgaaaccaa ctactcggca gcatttttaa ccggtagcca ggccgcgtgt
    58261 aaagcagcct gtaacgcctt taccgatgca gtgctggaaa tcgcgcgtaa tccaatccag
    58321 cgtgcgtaac ggaggttgcc gatgatcaat gcactgggat tgctggaagt ggacggcatg
    58381 gtcgccgcga tagatgcagc ggatgccatg ctcaaagcag ctaacgttcg tctgctcagt
    58441 cacgaagtgc ttgaccctgg tcgcttaacg ctggtggtgg aaggcgatct ggcggcgtgt
    58501 cgtgcggcgc tggacgcagg ttgtgctgcc gcgatgcgta ccgggcgtgt catcagccgc
    58561 aaggagatcg gtcggccaga cgatgacacc cagtggctgg ttactggctt taaccgccag
    58621 ccgaagcaac ccgtaaggga acccgacgcg ccagttatcg tcgcggaatc tgctgacgag
    58681 ttgttggcgc tgttaacatc agtacgtcag ggaatgacgg caggagaagt ggctgcccac
    58741 tttggctggc cgctggaaaa agccagaaat gcgctcgaac agctcttttc tgccgggacg
    58801 ttacgtaaac gcagtagtcg ttatcgtctc aagccccatt aacctgtcgg aggtgccggg
    58861 tgtcctacaa cacccggcat taacatcatg aaaaagaccc gtacagccaa tttgcaccat
    58921 ctttatcatg aacccttacc cgaaaacctg aagctcacgc cgaaggtcga agtggataat
    58981 gttcatcaac gacagacaac ggatgtctat gaacatgctt taacgattac cgcctggcag
    59041 cagatttacg atcagctgca tccgggcaag tttcattgtg aatttacgga aattctactc
    59101 gatgatattc aggtttttcg tgaatacacc ggtctggcgc tgcgtcagtc gtgcctggtc
    59161 tggccgaact cgttctggtt tggcattccg gcgacgcgcg gtgagcaggg atttatcggt
    59221 tcgcaatgtc tgggaagcgc ggaaatcgcc acccgccctg gtggcactga atttgaactg
    59281 agcacgccgg atgattacac gatcctgggc gtggtgcttt ctgaagatgt catcacccgg
    59341 caggctaact ttttgcataa cccggatcgg gtattacata tgctgcgtag ccagtcggcg
    59401 ctggaagtga aagagcagca taaagccgcg ctgtggggct ttgtccaaca ggcgctggcg
    59461 acgttttgcg agaacccgga aaatctccat cagcctgcag tgcgaaaagt gctgggggat
    59521 aatttgctaa tggcgatggg ggcgatgctg gaagacgcgc aaccaatggt gacggcggaa
    59581 agcatcagtc atcagagtta ccgtagatta ctttcccgcg cccgtgaata tgtgctggaa
    59641 aatatgtctg agccggtgac ggtgctggac ttgtgtaatc aactgcatgt cagtcgccgc
    59701 acgctacaaa acgcgtttca cgctatttta ggcattggcc caaacgcgtg gctgaaacgc
    59761 attcgcctga acgccgtacg ccgcgaactg ataagcccgt ggtcgcaaag cacaacggta
    59821 aaagacgccg ccatgcagtg gggattctgg catctggggc aatttgccac ggattaccag
    59881 cagctgtttg ccgagaagcc gtcgttgacg ttgcatcagc ggatgcggga atgggggtga
    59941 gggagttaca cccaatccct gaccttaata aactcactta acgccgcttc tgggctgcca
    60001 tcttttggct gataatcata ctcccagcgt accagtggcg gcattgacat caggatcgac
    60061 tcggtgcgcc cgccagtttg caggccaaac agagtgccgc gatcccagac cagattgaac
    60121 tcgacataac gaccgcgacg gtagagctga aaattgcact cgcgctcgcc gtaggccatc
    60181 gctttacgtc gctctacaat tggtaaataa gcgtcggtgt agcctttgcc taccgcctgc
    60241 ataaaggcaa aacagtggtc gaaatctggc gtgttcagat catcaaagaa cagcccgcca
    60301 ataccgcgct gttcgttgcg atgtttgagg tagaagtaat cgtcgcacca ctttttgtaa
    60361 cggggataaa cgtcttcacc aaatggcagg cacaggtcac gggcggtgcg gtgccagtga
    60421 atggcgtctt cttcaaaacc atagaaaggg gttaaatcga agccgccgcc aaaccaccag
    60481 acgggctctg cacccggttt ttcggcaata aaaaaacgca cattcgcgtg gctggtggga
    60541 acatacgggt tatgcggatg cactaccagt gaaacgccca tcgcctcgaa actgcgcccg
    60601 gcaagttccg ggcgatgagc ggtggcggaa gctggcatcg cctcgccgtg gacgtgagaa
    60661 aagttgacgc ctgcctgttc gaaaacacca ccattacgca acacccgact acgcccgccg
    60721 ccgccagctt cgcgctgcca actatcttcg acaaattctg cgccatcgac ggcggtcagc
    60781 tgctgacaaa tcgtatcctg aaggttgagc agaaactgtt taacctggtg tgcgtcgggt
    60841 ttcataactt atcgcttttt cgaatgtgct ttctggttgt cgaaccagtg gaaataactg
    60901 atcacgcctt cagcaatcgc tgtggcgatt ttctgacgaa acgccgccgt gcctaacagc
    60961 cgctcttctt ccgggttggt gataaacgag gtttccacca gcaccgaagg aaccgacggc
    61021 gatttcaaca ccacaaacgc ggcttgttcg gtgttgcggc tgtgcagttt atgcaccggc
    61081 ttaatctttt tcagaatatg cgagccgagc gtcaggctgt ttttaatagt atcggtttgc
    61141 accagatcaa acagcacttg ctgcaatagg tgatccttgt cagtcgcctt tttaccggca
    61201 acttcatcgg cgcggttttc gcgttcagac aggtatttcg ccattgcgct acttgcccca
    61261 cggttagaga gggcaaatac cgaagcaccg gcagctttcg ggttggtaaa gccatcggca
    61321 tgaattgaca taaacagatc tgcgccatgt ttatgggcga tttcaacgcg atcgtaaagt
    61381 gggataaacg tatcgccaga acgcgttaaa cgcgcatcaa tcccatgatt acgcaaaatg
    61441 gaacggacgt ttttagcaat cgccagcacc acatgttttt ctttcgaacc gttgcgtccg
    61501 atcgctccgg tatcaattcc gccgtggcct ggatcgagaa caacgacacg tttgccgcca
    61561 gattttttgg ctttcggctt gctgtgtccg ttgctggttt ttaaaggttc gtctttggcg
    61621 atggcttgcg acattcctga caacgtcagg gcagccaaac cggctttcag cacctggcgg
    61681 cgcgaagtga gtgtttttag tggtttaaaa gtgctcatac ggcctgagtt gtaataataa
    61741 agttccagat gttatatcgt atcgcgtaat ccgttacgac ggtttgatgt tgagaattgt
    61801 tttacttttc atttcaatag ttgacaactc cccattatgc cactttttca accgattttc
    61861 gtcagatatt ggctaattcg accgttcacg ccataatcag ccttttaacc cgcaaaaagg
    61921 taacgaatac catggagata cgcgtatttc gccaggaaga tttcgaagag gtcatcaccc
    61981 tttgggagcg ttgcgacttg ctgcgtccgt ggaacgatcc ggaaatggac atcgagcgta
    62041 agatgaacca tgacgtcagt ttgtttttgg tcgctgaggt aaacggtgaa gtggtcggaa
    62101 cggtgatggg cggttatgac gggcatcgcg ggtctgctta ttatcttggc gtgcatccag
    62161 agtttcgtgg gcgtgggatt gccaatgcgt tgcttaatcg gctggagaaa aagctgattg
    62221 ctcgtggctg cccgaaaatt cagatcaatg tgccggaaga taacgacatg gtgctcggaa
    62281 tgtatgaacg cctgggatat gaacacgccg acgtgctgag tctgggtaag cgtttgattg
    62341 aagatgaaga gtactgagtt ccatcctgtc cattatgatg cgcacggacg cctgcgttta
    62401 cccttgctct tctggcttgt gctattgctt caggcgcgaa cctgggtgct gtttgtcatt
    62461 gccggtgcgt cgcgcgagca gggtactgcg ctgctaaatc tgttttatcc cgatcacgat
    62521 aatttctggc tggggctgat tccaggcatt ccggcggtgc tggcgttttt gttgagcggt
    62581 cggcgggcta catttcctcg cacttggcat gtgctctatt ttctgttgct gttggcgcag
    62641 gtggttttac tttgctggca accgtggctg tggctgaacg gtgaatccgt tagcggtatc
    62701 ggtctggcgc tggtagtggc ggatattgtg gcgttaatct ggctgctgac caatcgacgt
    62761 ttacgcgctt gttttaatga agaaaaagaa taacggcact ttttggtgaa tttgcactcc
    62821 aagcaacgtt attgaataac caaaggaagt gacatgaaat cgctgcgttt aatgttatgc
    62881 gctatgccgt tgatgctgac cggctgttcc acgatgtcgt cagttaactg gtcggccgct
    62941 aacccgtgga actggtttgg gtcgtccacc aaagtgagcg agcagggcgt tggtgaatta
    63001 acggcgtcca caccactgca agaacaagcc attgccgatg cgcttgatgg cgattatcgc
    63061 ctgcgcagcg gaatgaaaac cgcgaacggc aacgtggtgc gcttttttga agtgatgaag
    63121 ggcgacaacg tggcgatggt gattaacggc gatcaaggca cgatcagtcg cattgacgtg
    63181 ctggatagcg atattcctgc tgacaccggt gttaaaatcg gtacaccgtt tagcgacctt
    63241 tacagcaaag catttggcaa ttgccaaaaa gctgatggtg atgataatcg tgccgtcgaa
    63301 tgtaaagccg aaggcagtca acatattagc taccagttca gcggggaatg gagtggtcct
    63361 gaagggttaa tgccttcgga cgataccctg aaaaactgga aagtcagtaa aattatctgg
    63421 cggcgttaat ttgcgtctga acaaaccgcc gccgcgaaaa aacacgtaaa atagcgctca
    63481 acaatgccac ggattgcgtg gcattattat tttcaggagg aacaatgtct caggttcaga
    63541 gtggcatttt gccagaacat tgccgcgcgg cgatttggat cgaagccaac gtgaaagggg
    63601 aagttgacgc cctgcgtgcg gccagtaaaa catttgccga caaactggca acttttgaag
    63661 cgaaattccc ggacgcgcat cttggtgcgg tggttgcctt tggtaacaac acctggcgcg
    63721 ctctgagcgg cggcgttggg gcagaagagc tgaaagattt tccgggctac ggtaaaggcc
    63781 ttgcgccgac cacccagttc gatgtgttga tccacattct ttctctgcgt cacgacgtaa
    63841 acttctctgt cgcccaggcg gcgatggaag cctttggtga ctgcattgaa gtgaaagaag
    63901 agatccacgg cttccgttgg gttgaagagc gtgacctgag cggctttgtt gacggtacgg
    63961 aaaacccggc gggtgaagag acgcgtcgcg aagtggcggt tatcaaagac ggcgtggatg
    64021 cgggcggcag ctatgtgttt gtccagcgtt gggaacacaa cctgaagcag ctcaaccgga
    64081 tgagcgttca cgatcaggag atgatgatcg ggcgcaccaa agaggccaac gaagagatcg
    64141 acggcgacga acgtccggaa acctctcacc tcacccgcgt tgatctgaaa gaagatggca
    64201 aagggctgaa gattgttcgc cagagcctgc cgtacggcac tgccagtggc actcacggtc
    64261 tgtacttctg cgcctactgc gcgcgtctgc ataacattga gcagcaactg ctgagcatgt
    64321 ttggcgatac cgatggtaag cgtgatgcga tgttgcgttt caccaaaccg gtaaccggcg
    64381 gctattattt cgcaccgtcg ctggacaagt tgatggcgct gtaagcgttt ctgattcatt
    64441 gattaaaggc caggcagtaa cattactgac tggccttttt attacttttg ctttaacgcc
    64501 gcatacacct gatcaactac ccaaccatac gttgcaatcg gcagctgacc gctttcgaac
    64561 atattgggat tcttttgcgg atcggcaacc ggcgaatgtg gcttgttgct taacatcaca
    64621 atcgccatat gattcaccgg atcgataacg gtcaccgttc cggtccagcc agtgtgaccg
    64681 taggtttgcg ggcttgccag cgtgccaaac gtcggcgtca tggtggcatt accattcacg
    64741 cgccagccga ggccaaaagt ggcatcttcc ttagagctgg tggtgaacat cttcaccgtt
    64801 tccgcactga acagctgcac atcgccatag cccccgccgt tcagcatcgt ttgcattaac
    64861 accgcaatat cgccggtatt ggaaaacaaa cctgcgtgcc cggaaacgcc gcccatcgaa
    64921 taaaaggctt tttcatcgtg cacctgaccc cagagagtgg aggtgcggat attcgggaaa
    64981 tggatcacgc catcgcgggt attgccgttc aattccgtgg cggcaatttg ctgcggttta
    65041 aagcctttca gcagcgggtt aaataccgta tgtgtcaggc cgagcgggcg ataaatcgac
    65101 tcttcaacat agcggtcaag cggctgaccg gtgaccgact cgacgataaa tccaagcagc
    65161 atataatcga catcactgta aatatgcttg ctgccgggct gatactccag cggcgtgcgc
    65221 ttgatcatct ccagcgtctg gcctttatcc tgggaatata acgcgcccgc gaccgcttta
    65281 ttcggatatt gtgggtccgc cgggaaaccg ccgctgtgat gcagcaggtc agaaatccgc
    65341 agcgtgtttt tgcctttgat ggtgtcattt ggactatcgg caaaccccgg aatatatttc
    65401 gcaatccgat cgtcaggatg cagtttgcct tcgctcatca gcttttgcag ggcgaagttc
    65461 gtggcgtaca ttttggtgtt tgaggccagg tcatacagcg tcccggtggt ggctttgaca
    65521 ggctgttcca tcaacacgct gccatcgtac tttttcgccg ctccccaggc tttgcgatac
    65581 acaatctgat tatctttaat gatcagcagg tttacgccgg gataaccggc gtccacttgc
    65641 tggctaatcc agcgatccat ctggttaagc cgttcgacgt tgaaccctgc tttctctggc
    65701 gaactttctg tcagaacagg gtattttgcg gcgctgacgc tacaactaac agccagcaga
    65761 gaaagataga gcattgtccg tttcatgata tcagtccaga ttgacgttac ggcagccaaa
    65821 gagcgtggtg aaaataaacc cgcaaaccca tgccaccaga atcccgccag cataaaccgc
    65881 cattgccgga aggatgcctt gtgcggaagt catcagcggc agcgccacca gaccagacgg
    65941 cccaaaggcg ctgtttaagc ccatcggcag accccaccag gctatcaggc cgataaacaa
    66001 accgcccgct gcgccgccta aacaggcggt aataaacggt ttcatgcggg gcagggtgac
    66061 gccgtaaatc aacggttcac caacgcccag cagaccggga ataatcgccc cgcgtacctg
    66121 actgcgtaat gcactgtgcg gttgcgcccg ccagtagagt gccagcgccg cgcccacctg
    66181 gcccgcgcct gccattgaaa ggatgggaaa taagctgttg aatccctggc tgtccattaa
    66241 cgcgaggtaa acaggaataa agccctgatg cacgccaaat accacggcga tcaggaacag
    66301 cccggctaaa accgcacaac cgaacggatt actgttcagg tgcataaaca gccatgacat
    66361 accttcgaat aaccagccac ccagcggcat aatgatcagg tacgcaagcg tagcggtgat
    66421 cagcagggtg attaacgagg tcagcagcat gtcgagatcg tccggcataa agcggcgcac
    66481 catgccttca atgcgggcgc aggcccacgc ggcaatcagc acgccgataa tattgccgcg
    66541 cggatcgatg ggcagaccaa agaaatcgtg aaaaccggcg tagtaaccgg tggttgcagc
    66601 ggggttataa ccgagcaaaa agagtgcggc aataattgcg ccattgacgc ccgtgccgcc
    66661 aaatgcctgg gcggcgttat agcccaccag aatcaccagg aaagtgaaca aacctttgct
    66721 gaacaccttc ataaaattca gcgcgtcggg tagcgtgccc tgagcatctg ccggaacgtg
    66781 catcaccgtg gcaattaacg tcgctatccc cagcagcaga ccggcggcaa taaaaccggg
    66841 gatcagcggc gtgaagatgg tggcgaattt ggcgagaaat tgttgcacgc cggaggtttg
    66901 tttggctttt aactgacgtt tgttctgggc ggcgatttct gcggcatcct gtaccggtgc
    66961 ctcgcccagc agctcgctca tcgcttttgc ggcacgatgc gctttaccag gcccaaaaac
    67021 gacctgcacc tggtcgctgg tcaaaatgac gcccttcacg ccttccagcg ttttgatatt
    67081 gggatcaacc agtgaactgt catgtacacc cagacgcagg cgcgtcatac agttaccaca
    67141 actggcgata tttcccggtc cgccgacacg ggtaagaatg gtgttcagaa gttcactgct
    67201 gatctctttg gccatgggtt attccttgtc taaaacctga cgaataaagc cgccgtgttg
    67261 atccaggcgt tttttagctt ctgcggcatc gagatttttc agcaccatca caatggccgt
    67321 tttacagttg cgctcgcagg caattaacgc cgcttccgct tgttcggcat tacatccggt
    67381 ggcgttttta acgatattga cctggcgcac atgcagtttt tcgttggtgg cgaccacatc
    67441 gaccatcagg ttgccgaaca ctttgccaga tttaatcatc agcccggtgg aaagcatatt
    67501 gagcaccagt ttctgcgctg tgcctgcttt cattcgtgaa gaaccagtga ccacttcggc
    67561 acctacaatc ggtgtaatgg caaactcggc ggtggtggaa acggcactcc ccggattaca
    67621 ggaaattccc actgtgcggc agccgagctg gcgtgcgtat tccagtccgg caatcacata
    67681 cggcgtgcga ccgctggcag caatgccaac caccacatcc tgtgccgtta aattaatatt
    67741 tttcagatcg ttaacaccac cttcccggct atcttcagcg ccttccaccg cgtgctgaat
    67801 ggcatattcg cctccagcaa tcaaaccaac caccagaccc ggtttcacgc cgtaggtggg
    67861 cggacattcg ctggcatcca gaatccccag acgaccggat gttcccgcac cgagataaat
    67921 cagacgaccg ccgccgctga cctgggcgtg gataacatcg atcgccgcgg cgatatccgg
    67981 caggacgcgc tcaacggcaa gcggtacggt tttatcttca tcgttgataa tccggcacat
    68041 ttccagcgtc gatacgcggt caatttcagc cgaggcggtg ttcgagcctt cagtaatcat
    68101 cttttctagt tgcatcggtg gtgaccttac tatttacgat caaataattg cagatgtatg
    68161 gaatcatata tcccgaattg acagacttaa aggaatcatt gagtctacac tcgcagagtg
    68221 tgaagagcgt cataagaact taagcggaat ttatgctgcc agcccaggag agtggaagat
    68281 gttgtacctg acgaaaataa gcaatgcggg gagtgaattc accgaaaacg agcaaaaaat
    68341 cgctgacttt ctgcaggcca acgtgagtga gctgcaatcc gtctcttcgc gcaagatggc
    68401 gaaacagctc ggcatcagcc agtcgagtat tgtgaagttt gcccagaagc tcggcgcaca
    68461 aggatttact gaattgcgta tggcgttaat tggcgaatac agtgccagcc gggagaaaac
    68521 gaatgccacg gcgctgcacc tgcacagttc gattaccagt gatgattcgc tggaagtgat
    68581 tgcccgcaaa ctgaatcgcg aaaaagagct ggcgctggag caaacctgcg cgttgttcga
    68641 ctacgcgcgg ctgcaaaaaa tcattgatgt catcagtaaa gcgcaattta ttcagataac
    68701 gggcctgggc ggatcggcgc tggtggggcg cgatctgtcg ttcaaactga tgaaaattgg
    68761 atatcgcgtg gcctgcgaag ccgacacgca cgtacaagcc accgtttccc aggcgttgaa
    68821 aaaaggcgat gtacaaatcg ctatctctta cagcggcagt aaaaaagaga ttgtcttgtg
    68881 cgcagaagcg gcacgaaagc agggggcaac ggtgattgcc attacttctc tggctgactc
    68941 tccgctgcgg cggctggcgc atttcacgct cgataccgtt tccggtgaaa ccgaatggcg
    69001 tagctcgtcg atgtctaccc gcacggcgca aaattctgtt accgatttac tgtttgttgg
    69061 tcttgtgcaa ctcaatgatg tcgagtcatt gaaaatgatt cagcgcagca gtgagctaac
    69121 acaacgcctg aaatgatgca taaagcagcg actggattga gattttcctg aattagtgag
    69181 ctgatccgca gcaatatttt gtttatcctg tattttcaga gggaatggag tgtaacgctc
    69241 tgtattaaca aggagagcat taaaatgggt aaactcacgg gcaagacagc actgattacg
    69301 ggcgcattgc agggaattgg cgaaggaatt gccagaactt ttgcgcgtca tggcgcaaac
    69361 ctaatcttgc tggatatctc ccctgagatc gaaaagctgg cggacgaact gtgtggtcgt
    69421 ggtcatcgct gtacggcggt tgtcgccgat gtgcgtgacc cggcgtcggt agccgcagct
    69481 atcaaacgcg cgaaggaaaa agaagggcgc attgatatcc tggtgaataa cgcaggcgtt
    69541 tgtcgtctgg gcagtttcct cgatatgagc gatgaagatc gcgatttcca tattgacatc
    69601 aatattaaag gcgtatggaa cgtcacgaag gcggtgctgc cagagatgat tgcgcgcaaa
    69661 gatggtcgca ttgtgatgat gtcttcagtc actggtgata tggtggccga tccgggtgaa
    69721 acggcgtacg ccttaacgaa agcggcgatt gttggcctga caaaatcgct ggcggtggag
    69781 tacgcgcaat ccggtattcg cgttaacgcc atctgcccgg ggtacgtccg cacgccaatg
    69841 gcggaaagca ttgcccgcca gtcgaacccg gaagatccag agtcggtgct gactgatatg
    69901 gcaaaagcaa tcccgctgcg tcgcctcgcc gatccgctgg aagtcggcga actggcggcc
    69961 ttcctcgcat cggatgaatc cagctattta accggtacac agaatgtgat tgatggcggc
    70021 agcacactgc cggagacggt tagcgtcggt atctgattca cctctgtttc ctccctgcat
    70081 gtgtggggag gatttcgttt taaacccggt tcttattccc ttttcaactt ccaaatcacc
    70141 aaacggtata taaaaccgtt actcctttca cgtccgttat aaatatgatg gctattagaa
    70201 agtcattaaa tatataaggg tgcgcaatgg ccgttaactt actgaaaaag aactcactcg
    70261 cgctggtcgc ttctctgctg ctggcaggcc atgtacaggc aacggaactg ctgaacagtt
    70321 cttatgacgt ctcccgcgag ctgtttgccg ccctgaaccc gccgtttgaa cagcaatggg
    70381 ccaaagataa cggcggcgac aaactgacga ttaaacaatc tcatgccggg tcatcaaaac
    70441 aggcgctggc gattttacag ggcttaaaag ccgacgttgt cacttataac caggtgactg
    70501 acgtacaaat cctgcatgac aaaggcaagc tgatcccggc ggactggcag tcgcgcctgc
    70561 cgaataatag ctcgccgttc tactccacca tgggcttcct ggtgcgtaag ggcaacccga
    70621 agaatatcca cgactggaac gacctggtgc gctccgacgt gaagctgatt ttcccgaacc
    70681 cgaaaacgtc gggtaacgcg cgttatacct atctggcggc atggggcgca gcggataaag
    70741 ctgacggtgg cgataaagcc aaaaccgaac agtttatgac ccagttcctg aaaaatgttg
    70801 aagtgttcga taccggcggt cgtggcgcaa ccaccacttt tgccgagcgt ggcctgggcg
    70861 atgtgctgat tagctttgaa tcggaagtga acaacatccg taaacagtat gaagcacagg
    70921 gctttgaagt ggtgatcccg aaaaccaata ttctggcgga attcccggtg gcgtgggtcg
    70981 ataaaaacgt gcaggccaac ggtacggaaa aagcagcaaa agcctacctg aactggctct
    71041 acagcccgca ggcgcagacc atcatcaccg actattacta ccgcgtaaat aacccggaag
    71101 tcatggacaa actgaaagat aaattcccgc agaccgagct gttccgcgtg gaagacaaat
    71161 ttggctcctg gccggaagtg atgaaaaccc acttcaccag cggcggcgag ttagacaagc
    71221 tgttagcggc ggggcgtaac tgatgtttgc agtttcatca agacgcgtgc tgccgggctt
    71281 taccttaagc ctcggcacca gtctgctgtt tgtgtgcctg attttgctgc tgccgctctc
    71341 cgcgctggtg atgcagctgg ctcagatgag ctgggcgcag tactgggagg tgatcaccaa
    71401 cccacaagtg gtcgcggcct acaaagtgac gctgctgtcg gcgtttgtgg catcgatttt
    71461 taacggcgtt ttcggtctgc tgatggcgtg gattttaaca cgttatcgct ttccgggccg
    71521 cacgctgctt gatgcgctga tggatttacc ctttgcgctg ccaacggcag tcgccggttt
    71581 aacgctggcc tcgctctttt ccgtaaacgg tttttacggt gaatggctgg cgaagtttga
    71641 tatcaaagtc acctatacct ggcttggtat tgcggtggcg atggccttta ccagcattcc
    71701 gtttgtggtg cgtaccgtgc agccggtgct ggaagagtta ggcccggaat atgaagaagc
    71761 ggcggaaacg cttggtgcaa cgcgctggca gagtttctgc aaagtggtgc tgccggaact
    71821 ttctccagcg ctggtggcgg gcgtggcgtt gtcgtttacc cgtagtcttg gtgagtttgg
    71881 cgcggtgata tttatcgccg ggaacatcgc ctggaagacg gaagtgacgt cgctgatgat
    71941 ttttgtgcgc ttgcaggagt ttgattaccc ggcggcgagc gcgattgctt cggtgatcct
    72001 cgcggcatcc ctgctgctgc tgttctcaat taacactctg caaagtcgct ttggtcggcg
    72061 tgtggtaggt cattaatggc ggaagttacc caattgaagc gttatgacgc gcgcccgatt
    72121 aactggggca aatggtttct aattggcatc gggatgctgg tttcagcgtt catcctgctg
    72181 gtgccgatga tttacatctt cgtgcaggca ttcagcaagg ggctgatgcc ggttttacag
    72241 aatctggccg atccggacat gctgcacgcc atctggctga cggtgatgat cgcgctgatt
    72301 gccgtaccgg taaacctggt gttcggcatt ctgctggcct ggctggtgac gcgctttaac
    72361 ttccctggac gccagttact gctgacgcta ctggacattc cgtttgccgt atcgccggtg
    72421 gttgccggtc tggtgtatct gctgttctac ggctctaacg gcccgctggg cggctggctg
    72481 gatgaacata atctgcaaat tatgttctcc tggcccggaa tggtgctggt caccatcttt
    72541 gtgacgtgtc cgtttgtggt gcgcgagctg gtgccggtga tgttaagcca gggcagccag
    72601 gaagacgaag cggcgatttt gcttggcgcg tctggctggc agatgtttcg ccgcgtcaca
    72661 ttgccgaaca ttcgctgggc gctgctttat ggcgtggtgt tgaccaacgc ccgcgcaatt
    72721 ggcgagtttg gcgcggtgtc ggtggtttcc ggctcgattc gcggcgaaac cctgtcgctg
    72781 ccgttacaga ttgaattgct ggagcaggac tacaacaccg tcggctcctt taccgctgcg
    72841 gcactgttaa cgctgatggc gattatcacc ctgtttttaa aaagtatgtt gcagtggcgc
    72901 ctggagaatc aggataaacg cgcacagcag gaggaacatc atgagcattg agattgccaa
    72961 tattaagaag tcgtttggtc gcacccaggt gctgaacgat atctcactgg atattccttc
    73021 aggtcagatg gtcgcgttgc tggggccgtc cggttccggg aaaaccacgc tgctgcgcat
    73081 tatcgccggg ctggagcatc aaaccagcgg gcatattcgc ttccacggca ccgacgtcag
    73141 ccgcctgcac gcgcgcgatc gcaaggtcgg tttcgtgttc cagcattacg cgctgttccg
    73201 tcatatgacg gtgttcgaca atatcgcttt tggcctgacg gtgctgccac gtcgcgagcg
    73261 cccgaatgcc gcagccatca aagcgaaagt gacaaaattg ctggagatgg tgcagcttgc
    73321 tcatctggca gatcgttatc cggcacagct ttccggcggc cagaaacagc gcgtggcgct
    73381 ggcgcgcgcg ctggctgtag aaccgcaaat tctgctgctt gatgaaccgt ttggcgcgct
    73441 ggatgcgcag gtgcgtaaag agctgcgtcg ctggctgcgt caactgcatg aagagctgaa
    73501 attcaccagc gtgttcgtga cccacgacca ggaagaggcg accgaagtag ccgatcgtgt
    73561 agtggtgatg agtcagggga atattgaaca ggctgatgcg ccgaatcagg tatggcgcga
    73621 accagcgacc cgttttgtac tcgaatttat gggcgaagtg aaccgcctgc agggaaccat
    73681 tcgcggcggg cagttccatg ttggcgcgca tcgctggccg ctgggctaca cacctgcgta
    73741 tcagggtccg gtggatctct tcctgcgccc gtgggaagtg gatatcagcc gccgtaccag
    73801 tctcgattcg ccgctgccgg tacaggtact ggaagccagc ccgaaaggtc actacaccca
    73861 attagtggtg cagccgctgg ggtggtacaa cgaaccgctg acggtcgtga tgcatggcga
    73921 cgatgccccg cagcgtggcg agcgtttatt cgttggtctg caacatgcgc ggctgtataa
    73981 cggcgacgag cgtatcgaaa cccgagatga ggaacttgct ctcgcacaaa gcgcctgata
    74041 ggttgagtga atgttaaacg cccggaggcg cttcccgcga tccgggcttt ttaatggcaa
    74101 ggtttgtaac ctgtagacct gataagacgc gcaagcgtcg catcaggcaa caccacgtat
    74161 ggatagagat cgtgagtaca ttagaacaaa caataggcaa tacgcctctg gtgaagttgc
    74221 agcgaatggg gccggataac ggcagtgaag tgtggttaaa actggaaggc aataacccgg
    74281 caggttcggt gaaagatcgt gcggcacttt cgatgatcgt cgaggcggaa aagcgcgggg
    74341 aaattaaacc gggtgatgtc ttaatcgaag ccaccagtgg taacaccggc attgcgctgg
    74401 caatgattgc cgcgctgaaa ggctatcgca tgaaattgct gatgcccgac aacatgagcc
    74461 aggaacgccg tgcggcgatg cgtgcttatg gtgcggaact gattcttgtc accaaagagc
    74521 agggcatgga aggtgcgcgc gatctggcgc tggagatggc gaatcgtggc gaaggaaagc
    74581 tgctcgatca gtttaataat cccgataacc cttacgccca ttacaccact accgggccgg
    74641 aaatctggca gcaaaccggc gggcgcatta cccattttgt ctccagcatg ggaacaaccg
    74701 gcactatcac cggcgtttca cgctttatgc gcgaacaatc caaaccagta acgattgttg
    74761 gcctgcagcc ggaagaaggc agcagtattc ccggcattcg ccgctggcct gcggaatatc
    74821 tgccggggat tttcaacgct tctctggtgg atgaggtgct ggatattcat cagcgcgatg
    74881 cagaaaacac catgcgcgaa ctggcagtgc gggaaggaat attctgtggc gtcagctccg
    74941 gcggcgcggt tgccggagca ctgcgggtgg caaaagctaa ccctggtgcg gtggtggtag
    75001 cgatcatctg cgatcgtggc gatcgctacc tttccaccgg ggtgtttggg gaagagcatt
    75061 ttagccaggg ggcggggatt taagattatt gcgcaggcac cgttttatcc gtctgcgcaa
    75121 ttagcgcatg taaaaaaggc tgtgcgttct catcgctctt accggggatc ttcacaatat
    75181 acggcttccc ggtaatcgat gacgacgaag caaccttatc aataaactgc tcggcagtgt
    75241 caatgcggtt acgggtattg cccagcttca gacgcagatg agaaaccgct tcatcgcagg
    75301 tatgttcatc accgttgcgc acaaatatca aatccttttt ctgtgctaat ccctccagca
    75361 tggcgttgat gcgggcttct tcatgggcag ttaacttcgc gtaagcgggg agtgtcatta
    75421 gtagtgtaat gaccagacaa atgattttct tcattgaatt accttatcct tgttttgtga
    75481 tagccgattt gactgccaga atcaggtaaa gtttcggttg gaatacgatg aggatcatcg
    75541 gcaaggagaa aaaattaaat gagtagtttg ttgttgttta acgataagag cagggcgctg
    75601 caggcggata tcgtcgccgt gcagtcgcag gtggtttacg gcagcgtggg caacagcatt
    75661 gccgtgcctg ctatcaaaca gaacggtctg aatgtttttg ccgtgccgac ggtattgctg
    75721 agcaatacgc cgcattatga cactttctac ggtggtgcga ttccggatga atggtttagc
    75781 ggttatttgc gtgcgcttca ggagcgtgat gcgctgcgcc agctgcgcgc tgtaaccacg
    75841 ggctatatgg gaacggcatc gcaaatcaaa atccttgccg agtggctgac tgcgctacgc
    75901 aaagaccatc ctgacctatt gatcatggtc gatccggtga ttggcgatat tgatagcgga
    75961 atttatgtca aacctgacct tcccgaggcg tatcgacaat atttactgcc gctggcgcag
    76021 ggaattaccc ccaatatctt tgagctggaa atcctgaccg gtaaaaattg ccgcgatctc
    76081 gacagtgcca ttgctgccgc aaaaagtctg ctttcagaca cattaaaatg ggtggtgatt
    76141 accagcgcct ccggtaatga agaaaatcag gagatgcagg tggtggtggt cagtgccgac
    76201 agcgtgaatg tcatttccca ttcacgggta aaaaccgacc tgaaagggac tggcgacctg
    76261 ttttgtgctc agcttatcag tggtttgctg aaagggaagg cgttaaacga tgcggtgcac
    76321 cgtgcggggt tgcgcgtact ggaagtgatg cgctacaccc agcagcatga gagcgatgaa
    76381 ttgattttgc cgccgctggc ggaagcataa aaaatggcgc cgatgggcgc catttttcac
    76441 tgcggcaaga attacttctt gatgcggata accggggttt cacccacggt tacgctaccg
    76501 gacagtttga tcagttcttt gatttcgtcc atgttggaga taacaaccgg agtcagggta
    76561 gacttggctt tctcttccag tagcggcaga tcaaattcaa tgacagtatc gccaactttc
    76621 acgcgctgac cttcttcagc aatacgcttg aagccttcgc ctttcagttc aacggtgtcg
    76681 ataccgaagt ggacgaacag ttcaacgccg ctatcagatt cgatagagaa cgcgtgattg
    76741 gtttcaaaga ttttaccaat ggtaccgtct acaggcgcga ccattttgtt acccgttggt
    76801 ttgatagcaa taccatcacc aacgattttt tccgcaaaaa cgacatccgg cacgtcttcg
    76861 atattgacga tctcgccaga gagcggagca atgatctcaa tagttccggt atccttcttg
    76921 tcgtcggaaa ccagagattt cagtttatcg aacaaaccca tgatcttctc ctaagcagta
    76981 aattgggccg catctcgtgg attagcagat tgttttttct tcaatgaact tgttaaccag
    77041 cgtcattaac tcgtccgttg tcggttgagc aagagcctgc tctgctaaca ccttcgcatc
    77101 ttcgaagttc gtgttacgga taatcttctt aatgcgcggg atagaaatgg cgctcataga
    77161 gaattcgtcc agacccatcc ccagcaacag aagtgtagca cgttcatcgc cagcaagctc
    77221 accacacatg ccagtccatt tgccttcagc atgagaagca tcaataactt gcttgatcaa
    77281 gttcagcacg gacggtgaca ttggttggta aaggtgtgaa atcatatcat taccacggtc
    77341 aactgccaga gtgtactgcg ttaaatcatt ggtgccgata ctaaagaaat caacttcttt
    77401 ggctaaatga cgtgcaattg ttgcggcagc cggtgtttcc accattacgc cgatttcaat
    77461 tgactcgtca aacgctttac cttcgtcgcg cagttcctgt ttgtagattt cgatctcttt
    77521 gcgcagtgca cgcacttctt caacagagat gatcatcggg aacataatgc gcaatttacc
    77581 gaaagccgag gcacgcagga tagcgcggag ctgatcgcgc aggatctctt tacgatccat
    77641 cgcgatacgg atagcgcgcc agccgaggaa cgggttctct tctttcggga agttcatgta
    77701 tggcagctct ttgtcgccgc cgatgtccat ggtacgaacg ataaccgcct gcgagccaca
    77761 cgcttcagcc actgctttgt aagcagcaaa ctgttcttct tcagtgggca gcgcgtcgcg
    77821 gtccatgaac aggaactcag tacgatacag accaacgcct tcagcgccgt tacgctctgc
    77881 accttcaacg tcacgaaccg taccaatgtt agcgcacact tctacctggt gaccgtccag
    77941 cgtaatagct ggcagatctt tcagtttagc aagctctgct ttttcagaag ccacttgctc
    78001 ctgaacagcg cgcattttat caataacttc gttggttgga ttgacgtaaa cctgattatt
    78061 tacggcatcc agaatcagat agtcgtcatt tttcacctga gaggtgacgc taccggtacc
    78121 cacgatagca ggcagttcca gagaacgcgc catgatagag gtgtgggaag tacggccacc
    78181 cgcgtcggtg atgaaaccca gcaccttctt caggttcagc tgtgcggttt cggacggcgt
    78241 caggtcagcg gcaaccagaa tgacttcatc ctgaatggcg ctcaggtcga taatcttcag
    78301 gcccaggatg ttgcgcagca ggcgcttacc gatatcacgt acgtcagccg cacgttcttt
    78361 caggtattca tcatccagct cttccagggc agaagcctga ccttcgataa cttcatgagc
    78421 agctgcgtca gctgtcatgt gcttatcttt aatcagggct atgatttcct gctccagctc
    78481 ctcatcttcg agcagcataa tatgcccttc aaagatggct tctttttctt caccgaacgt
    78541 ttcaccagct ttcgttttga tcgtttccag ctgggctgat gccttggcac gaccgctcag
    78601 aaaacgttca acttcctgat caacctggtc ggcagaaatt tttttccggt caatgacaat
    78661 ttcgtcttct ttcagaagca gagctttacc gaaagcgata cccggggatg ctaaaatgcc
    78721 tgaaatcata accctacctt acttgtgact gatttttaaa agaacccggg aaattactcg
    78781 agttccgcca tcagtttaac cagatgttca accgctttct gctcgtcttc gccttctgcg
    78841 gagatagtca caacggtacc ttgagtcagg cccagagtct gcagtttaaa caggcttttc
    78901 gcgctggcgc ttttgccgtt ggaagtcaca gtaatttcag aagtgaagcc cttagcttct
    78961 tttacaaact gggcagcagg gcgggtgtgc agaccgttcg gagcggtaat ggtaacttct
    79021 tgctggaaca ttgtatttcc ccaacttata ggtttagtgt tgtggaacta aagtctagcc
    79081 tggcggttcg actttagcct gtattgttag cgccagtttt taacagacgc gacgcacgaa
    79141 gagcgcaata cagtccattg ctggcgggtg ttgaccgctg acgtttcgta cgtcattaaa
    79201 cattatgccg ctaaaggaag aattgaacca aatcataaaa tcgattcagc tttggctttt
    79261 cctgtaacga ttaatttcgc gcatcaaaat aattagtctg gttaaatacc agttccgaca
    79321 ggctgaatca atgccaggag gggcgaaagt ttgaagcagg ccacaaaaaa gcacctaaaa
    79381 aggtgctttt ttacgcattt ttaacaagct ggcattactg ttgcagttct ttctcagtga
    79441 agagatcggc aaacaatgcg gtgcttaaat aacgctcacc cgatgacggt agaataacca
    79501 caatattctt gttggtaaag ctttcatctt cttgtagttt caacgccgca gcaacagctg
    79561 ctccagaaga gatacctgca agaatacctt cttcttccat cagacgacgc gcggtagaaa
    79621 tcgcttcttc attggtgatg ccaatgactt tatcgaccag cttgagatcg aggttagctg
    79681 ggataaaacc agcgccaata ccctgaattt tatgcgggcc aggtttaatc tcttcaccag
    79741 ccagtgcctg ggcgataact ggagaatcgg ttggctcaac ggcgacagag ataagatcgg
    79801 tcttgccttt ggtgccttta atgtagcggc tgacgccagt cagcgtaccg ccagtcccaa
    79861 cgccagcaat aaatacatca acctgaccgt cggtatcttc ccagatctcc ggaccggtgg
    79921 ttttttcgtg aatttcaggg tttgccggat tgctgaattg ttgcagcagc aggtatttct
    79981 ctggattgct ggcgacaatt tcttctgctt tttggattgc gcctttcatg cctttagcac
    80041 cttccgtcag caccaggttt gcacctaacg ctttcagcag cttgcggcgt tcaatactca
    80101 tggtttctgg catggtcagg gtgagtttgt aaccgcgagc ggcagctaca taggccagtg
    80161 caatcccggt attaccgctg gtcggttcaa ccagttcaac gcctggtttc agcacgccgc
    80221 gcttttcggc atcccaaatc atgttggcac cgatacggca cttaacgctg aagctggggt
    80281 tacgagattc caccttcgcc agaatgcgtc cgttaccgat gcgattcagg cgaaccagcg
    80341 gcgtgtgacc gatagtcagc gagttatctt caaaaatctt actcatggcc tgtccttaac
    80401 tgtatgaaat tgggatacaa caggtagcat acccgctcag agaatatgcg gaagtaagga
    80461 tttagcatat ctatatacag aagggaaata atgacatgca agatggaata aggggcggca
    80521 taagccaccc ctgtttcaca caaacggttt acaaaataac cggtagattg ttaccgccac
    80581 atcgcgtgtt tatcgcgata gcaatcgacc cacatcgccg tcgcgccaca aacggcaacg
    80641 ggcatgatga acagattaag cagcgggatc atcgtaaaca ggctggttaa agcaccaaac
    80701 tgcatattgg tgattttgcg tgtgcgcagg gcggtgcgca tctctttaaa cggcactttg
    80761 tggttatcga aggggtaatc gcaatactgg atggctaaca tccaggcgct aaacaggaac
    80821 cacagtaccg gcgcgacggt ttgcccaata ccagggatta agtaaagaat tagcaataca
    80881 attgcgcgcg gcagatacca ggcaaatttt tgccattcgc gtttcatgat tcgcggcaca
    80941 tctttcatga taccgaaaat cccggtatct ggcggtgtag cgccagtcaa tcgtgcttcc
    81001 agttgttcag ccaataaacc gttaaacgga gcggcaatcc agttagcaat cgtggagaag
    81061 aaatagccaa acactaacag cacagagatg acagccagag gccacaacag ataactcagc
    81121 cattgtagcc agtccggaac gtaactcatg agagtcggga tccagacatc gagctgtgta
    81181 aagagccacc agaatgcgcc ccccatcaac aaaatattga ccagcagcgg taaaataacg
    81241 aaacgccgaa tcccaggttg cgagacgagc ttccagcctt gcgcaaaata gtaaaaaccg
    81301 ctgcgtgggg cagatgtgaa tgatgaaacc ataatcagga tgagctcctt ttgaccaatc
    81361 ccaggaaaat tctgcgtatt ttaccgggta attgcgcaat ggacagttag gatatgttcg
    81421 aaaaaacagc aaaaagcacg atttcatcta tctttgtgct gtgaaagtta atagtgcact
    81481 tgcacttgag gtaatcggca aatactctta gtgagtaaat gtttgccgtg gtggcaaggt
    81541 gttagaacaa cagagaatat aatgatgcag gatttgcgtc tgatattaat cattgttggc
    81601 gcgatcgcca taatcgcttt actggtacat ggtttctgga ccagccgtaa agaacgatct
    81661 tctatgttcc gcgatcggcc attaaaacga atgaagtcaa aacgtgacga cgattcttat
    81721 gacgaggatg tcgaagatga tgagggcgtt ggtgaggttc gtgttcaccg cgtgaatcat
    81781 gccccggcta acgctcagga gcatgaggct gctcgtccgt cgccgcaaca ccagtaccaa
    81841 ccgccttatg cgtctgcgca gccgcgtcaa ccggtccagc agccgcctga agcgcaggta
    81901 ccgccgcaac atgctccgcg tccagcgcag ccggtgcagc agcctgccta tcagccgcag
    81961 cctgaacagc cgttgcagca gccagtttcg ccacaggtcg cgccagcgcc gcagcctgtg
    82021 cattcagcac cgcaaccggc acaacaggct ttccagcctg cagaacccgt agcggcacca
    82081 cagcctgagc ctgtagcgga accggctcca gttatggata aaccgaagcg caaagaagcg
    82141 gtgattatca tgaacgtcgc ggcgcatcac ggtagcgagc taaacggtga actgcttctt
    82201 aacagcattc aacaagcggg cttcattttt ggcgatatga atatttacca tcgtcatctt
    82261 agcccggatg gcagcggccc ggcgttattc agcctggcga atatggtgaa accgggaacc
    82321 tttgatcctg aaatgaagga tttcactact ccgggtgtca ccatctttat gcaggtaccg
    82381 tcttacggtg acgagctgca gaacttcaag ctgatgctgc aatctgcgca gcatattgcc
    82441 gatgaagtgg gcggtgtcgt gcttgacgat cagcgccgta tgatgactcc gcagaaattg
    82501 cgcgagtacc aggacatcat ccgcgaagtc aaagacgcca acgcctgata cacttaaggc
    82561 aaattaactc ctcttcgaac ccccgcttgt cgggggtttt tagcattgat ggtgcgatat
    82621 ggaatcaatc gaacaacaac tgacagaact gcgaacgacg cttcgccatc atgaatatct
    82681 ttatcatgtg atggatgcgc cggaaattcc cgacgctgaa tacgacaggc tgatgcgcga
    82741 actgcgcgag ctggaaacca aacatccaga actgattacg cctgattcgc ctacccaacg
    82801 tgtaggcgct gcgccgctgg cggctttcag ccagatacgc catgaagtac caatgctgtc
    82861 actggataac gtttttgatg aagaaagctt tcttgctttc aacaaacgtg tgcaggaccg
    82921 tctgaaaaac aacgagaaag tcacctggtg ctgtgagctg aagctggatg gccttgccgt
    82981 cagtattctg tatgaaaatg gcgttttagt cagtgccgcg acccgtggcg atggcaccac
    83041 cggggaagat atcacgtcta atgtgcgtac tattcgcgct attccgctga agctgcacgg
    83101 agagaatatc ccggcgcgtc tggaagtgcg tggtgaagtg ttcctgccgc aggcggggtt
    83161 cgaaaagatt aacgaagatg cgcgacgcac gggcgggaaa gtttttgcta acccacgtaa
    83221 tgcggcagct ggttcactgc gtcagcttga tccgcgtatt acagcgaagc gaccgctcac
    83281 ttttttctgc tatggcgttg gtgttctgga aggtggcgag ctgccggata ctcatcttgg
    83341 ccgtttactg caatttaaaa agtgggggtt gccggtcagc gatcgggtaa cgctttgtga
    83401 atcggcggaa gaagtgctgg cgttctatca caaagtggaa gaagaccgcc cgacgctggg
    83461 ctttgatatc gacggcgtgg tgattaaggt caactcactg gaacagcagg agcagcttgg
    83521 ctttgtcgcg cgtgccccgc gctgggcggt agcgtttaaa ttcccggcgc aggagcagat
    83581 gacctttgtg cgtgacgtcg agtttcaggt tgggcgtact ggcgcgatta cgcctgttgc
    83641 gcgtctggaa cctgtccatg ttgctggcgt gctggtgagt aacgcaacct tacacaatgc
    83701 ggatgaaatc gaacgtcttg gtttacgcat tggcgataaa gtggtgattc gccgcgctgg
    83761 cgacgtgatc ccgcaggtgg ttaacgtcgt gctttctgaa cgcccggaag atacccgtga
    83821 ggttgtattc ccgacgcatt gtccggtatg tggttctgac gttgagcgtg tggaaggtga
    83881 agcggttgcc cgctgtaccg gtggcctgat ttgcggtgcg cagcgtaaag agtcgctgaa
    83941 acactttgtt tcccgccgtg cgatggatgt tgacggaatg ggcgacaaaa tcatcgatca
    84001 gctggttgaa aaagaatatg tccatactcc ggcagatctg ttcaaactca ccgcaggcaa
    84061 actgaccgga ctggagcgta tggggccaaa atcggcacaa aacgtggtta acgcgctgga
    84121 aaaagcgaaa gaaaccacct ttgctcgctt cctctatgca cttggcatcc gtgaagtcgg
    84181 cgaggccacc gcagcaggtc tggcggcata tttcggcacg ctggaagcgc tggaagccgc
    84241 ttcgattgaa gagctgcaaa aggtgcctga tgttggcatt gtcgttgcat cccacgttca
    84301 caacttcttt gccgaagaaa gcaaccgcaa tgtcatcagc gagctgttgg cggaaggtgt
    84361 tcactggcct gcgccgatcg ttatcaacgc ggaagagatt gacagcccgt ttgctggtaa
    84421 aaccgtggtg cttacgggca gcttaagcca gatgtcgcgt gatgacgcta aagctcgact
    84481 ggttgaactg ggcgcgaaag tcgcgggcag cgtgtcgaag aaaaccgatc tggtgattgc
    84541 cggagaagcg gcaggttcta aactggcgaa agcgcaggaa ctgggcattg aagtcatcga
    84601 cgaagcggaa atgctgcgtt tgctgggtag ctgagatgga aaaagagcag ctgattgaaa
    84661 tagccaatac gataatgccg tttggcaaat ataaagggcg tcgcttaatc gacctgccgg
    84721 aggagtatct gctgtggttt gcccgcaaag atgaattccc ggcagggaag ctcggtgagc
    84781 taatgcaaat cacgctgctg attaaaaccg aggggctgac gcaactggtc cagcccctga
    84841 agcgtccgct ttaagcttta tcggcgctgc tttcctgctg tgcctgtaac tgttcggtct
    84901 ggcgtttgta tcgacgcgcc agcaccgcac agaccatcaa ttggatctga tggaaaatca
    84961 ttaggggcag caccatcata ccgatcaccg atgtggggaa cagaatgttt gccatcggga
    85021 tgccatttgc cagactcttt ttcgaaccac aaaagacgat agtaatttca tctgccttat
    85081 tgaagcccag tcggcgtgcc ataaagacgt taactacaat cacgatagcc agaagaacgc
    85141 agctgaccac cacgataaac agcaatgatc cccagccaac tttatgccag ataccattaa
    85201 cgacggcttc gctgaatgct gtataaacca ccaacagaat ggacgtctgg tcagttttcg
    85261 caatccattt tttattgcgc gacacccagt caccaatcca cggccgggaa agatgcccca
    85321 acacaaacgg cagcagcagt tgcagcataa ttttaccgac ctgctcaagg ctgccccctg
    85381 caccgtgaac attcatcacc agaccaacca gcaatggtga aaggaaaatc cccagcaggc
    85441 tggatgctga cgcagaacaa accgccgccg cgacgttacc gcccgccatt gacgtgaagg
    85501 cgattgcaga ctgcacggta gccgggagaa tgcacaagta gagaaaaccg gagtagagca
    85561 tcgggtcgac atttaccggt ttccaccagg caaacagtac acccagaatc ggaaacagca
    85621 cgaaggtgct gcaaattacc cacaaatgca gtcgccagtg accaccgcca gcaataatcg
    85681 cctcacgcga caacttcgcg ccgtgcataa agaacagcag ggcaatcgct gcggtggtca
    85741 gattttcaaa gaaggggacg aaatcgcctc tggccggaaa gaaagaggcc agcaacacca
    85801 ccgtgatcag ggttaaggtg aaaggatcga ggatacgaaa aagtttcata aagactccag
    85861 atgatcgatg gttctattgt gcttcttcca gtttgagaaa taaaattgat ttattgcatc
    85921 cattcatgaa gaaaacagat gaattattct ttaaaacaat taaaggtttt cgtcacagta
    85981 gcgcaggaga aaagttttag tcgtgcagga gagcgtatcg gcctgagcca gtcggcagtg
    86041 agtcacagtg tgaaggaact ggaaaatcat actggtgttc gcctgctgga cagaaccacg
    86101 cgtgaagtgg tgcttacaga tgcagggcag cagctggctt tgcgtcttga gcgactgctg
    86161 gatgaactga acagcacgtt gcgcgatacc gggcgtatgg ggcaacaact gagcggaaaa
    86221 gttcgggtcg ctgccagcca gaccatttcc gcgcatctta ttccgcaatg cattgctgaa
    86281 agccatcgcc gctatccaga tattcagttt gtcctgcacg atcgtccgca gcagtgggtg
    86341 atggaaagta ttcgccaggg agatgtcgac tttggcatcg tcatcgatcc aggcccagtg
    86401 ggcgatctgc aatgtgaagc gattctttcc gagcctttct ttctgctttg ccatcgcgat
    86461 agcgctttgg ccgtcgaaga ttatgtaccc tggcaggcat tacagggagc gaaattggtg
    86521 ttgcaggatt acgcgtcagg cagccgaccg ctgattgacg cagcgctggc gcgcaatggt
    86581 attcaggcga atattgtgca ggagattgga catccggcga cgctgttccc gatggtagcc
    86641 gcaggcatcg gcatcagtat tcttcccgca ctggcgctac cattgcctga aggtagccca
    86701 ctggtggtaa agcgcattac gccggttgtt gaacgccagt tgatgctggt gcgcaggaag
    86761 aaccgttcac tttccaccgc cgctgaagcg ttatgggacg tggtacgaga ccagggcaat
    86821 gcgctaatgg ctggtcggga aggggatccg ttgtatcaga tatagagtga attcatactg
    86881 ccgggagccc cggcagtaat caatcagaag ttaaatttga tggagtagat tatcgcatcc
    86941 tggtaaaaat cttcgcccag tttgtcatcc gcatatcgat actgaatccc cgtagtaatg
    87001 tgtgatgttg cattccacca cagtgcaact gcgccattca gcccttcttt accgccatta
    87061 cccgctgcat aggtagcgtc acggtcaaac tcgtactcgt tccagttggt aagagtgaat
    87121 ttctcgctgc ccagcataaa gttgtaacct gcaacccagc cggcgacata gccgttgtcg
    87181 ccagtgtaat aggtttgatc tgtataacgc ttagcaaaga acggtttgaa ccaccaaccg
    87241 ctgccggtga aattgtagcc gataccgtac aggaacatat cgtcgtggaa attcacgcga
    87301 ttcgcagaac cgtaggtgcc atacgcatgc aagtagagat taaatccggt gtcacccagg
    87361 taaatacggt tggtattttt aaaggtataa cgctgctcgc tacctggttt attatggcga
    87421 ccgttataga agttttccca gtcgaagaac ccgtacattt ctccccaact aaagttagcg
    87481 ccaccttcaa attcaagata accaaaatca tctttgtgtg atttggtcga cgatttttcg
    87541 gtagtccggc tggtccagtc cagataatgc acgccgatat ctgcaaaacc gcctttaaat
    87601 tctgcatgag atacaacagg tatcgctaat atagaggaaa gtgtcagagt taaaagatgt
    87661 tttttcatag cataattccc tatgccgatc gttattagta gattgcttaa taaagtggtt
    87721 tgctaaaaac gacatattgt taatgcaaag gaattaatat cgccaataca gtttttaccg
    87781 tggattaata gagaaaagat ataaatccat cgatatcgaa tcgctattga atgcgtgaga
    87841 aaagtgtatt ggtaagagcc gggacaacct cgctataaaa aggcgttaga ttccacccta
    87901 cagaaaaagg atatgtatgt ctcaggttca attttctcat aacccgctgt tttgcataga
    87961 tattatcaag acttataaac ctgatttcac gccacgagtg gcctttattt taggttccgg
    88021 gctcggcgcg ctggccgatc agattgagaa cgctgtcgca atttcctacg aaaagctgcc
    88081 gggcttcccg gtaagtacgg tacatggtca tgcgggggag ttagtgctgg gccatctcca
    88141 gggcgtaccg gtggtatgta tgaaaggtcg cggacatttc tacgaaggtc gtggaatgac
    88201 cattatgact gacgcaattc gtacctttaa gctgctgggc tgcgagctac tgttctgcac
    88261 caatgcggca ggctcgctgc gtccggaagt gggggcaggc agcctggtcg cattgaaaga
    88321 tcatatcaac accatgcccg gtacgccgat ggtgggtctt aacgatgatc gttttggaga
    88381 gcgcttcttc tcactggcaa atgcctacga tgcggaatac cgcgcactgt tacaaaaagt
    88441 ggcgaaagaa gagggtttcc ctctgacgga gggcgtgttc gtctcgtatc cggggccgaa
    88501 tttcgagact gcggcggaaa ttcgcatgat gcaaattatt ggtggggatg ttgttggtat
    88561 gtctgtggtg cctgaggtta tttcagctcg ccattgcgac cttaaagtcg ttgcggtctc
    88621 tgcgattacc aatatggcgg aaggtttaag cgatgtgaaa ttgtcacatg cgcaaacgct
    88681 ggcggctgcg gagctctcta agcagaactt catcaacctt atttgtggct ttctgcgcaa
    88741 aattgcctga gaaaaacaat aagaaacggc ctcgcgggtg aggccaaatg atgcgacaag
    88801 gaaacgatta tgagcatcgc gatgcgctta aaggtaatgt cctttttgca atattttatc
    88861 tgggggagct ggctggttac cctcggctct tacatgatta atacccttca tttcaccggc
    88921 gctaatgttg gcatggttta cagttccaaa gggatcgccg cgattattat gcctggtata
    88981 atggggatca tcgcagacaa atggctgcgc gcagaacgtg catacatgct gtgtcacctg
    89041 gtgtgtgcgg gcgtactttt ttatgcggca tccgtaactg atccggatat gatgttttgg
    89101 gtgatgttag tcaatgcgat ggcgtttatg ccgactattg cgttatcgaa cagcgtctct
    89161 tattcctgtc ttgcccaggc agggcttgac ccggtgaccg ctttcccgcc cattcgcgtt
    89221 tttggtacgg tggggttcat tgtcgcgatg tgggcagtaa gcctgctgca tctggaattg
    89281 agtagtctgc agctgtatat cgcgtccggt gcgtcattgc tgctgtcggc ttatgcgctg
    89341 actttgccga agattccggt tgcggagaaa aaagcgacca catcgcttgc cagcaagctg
    89401 ggtctggatg ccttcgtgct gtttaaaaat ccacgcatgg ccatcttttt cctctttgcc
    89461 atgatgctgg gtgcggtact gcaaattacc aacgtttttg gtaatccgtt cctacatgat
    89521 ttcgcccgta acccggagtt tgctgacagt tttgtggtga aatatccctc cattttactg
    89581 tcagtttcac agatggcaga agtgggcttt atactgacta tcccattctt tttaaagcga
    89641 tttggcatta aaaccgtcat gctgatgagt atggtggcct ggacgctgcg ctttggcttc
    89701 ttcgcctatg gcgatccgtc aacaaccgga tttattttgc tgctgctgtc gatgattgtt
    89761 tatggctgtg cattcgattt cttcaatatt tctggttcgg tatttgtcga acaggaagtt
    89821 gattccagca ttcgtgccag cgcgcagggg ctctttatga ccatggtaaa tggtgtcggc
    89881 gcatgggttg gctcgattct gagtggcatg gcagtagatt acttttcggt ggatggcgta
    89941 aaagactggc aaactatctg gctggtgttt gcaggatatg ctctttttct cgcagtgata
    90001 tttttctttg ggtttaaata taatcatgac cctgaaaaga taaagcatcg agcggtggct
    90061 cattaaaagg attcgcagct ctgctcttca gagctgcttt tatgataaag gttaattagt
    90121 gaaagatatt ttattttctt ccggcgttgg ttttggcatt ggtgctttgt ttacaatcgt
    90181 gcgcttgcct attcctgtac caaatgtatt accaggtata ttatccattg tgtttatgta
    90241 tgtcggatat ctggtggtga aatactttat gccatgataa tttaatacga tgtatttatt
    90301 atatggagca cttaattatg gaacgcgtat acagaacaga tcttaagttg ctccgttatt
    90361 ttcttgccgt agcggaagag ttgcattttg gccgcgcagc agcgcgttta aatatgtctc
    90421 agcctccgct cagtattcat attaaagagc tggaaaatca actcggcacg cagcttttta
    90481 ttcgccattc gcgcagcgtc gtactgacac acgcgggcaa aatcttgatg gaagaatcgc
    90541 gtcgattgct ggtgaatgct aataatgtat tggctcgggt tgaacaaata ggtcggggag
    90601 aagcagggcg gattgaactc ggcgtagtgg gaacggctat gtggggacgg atgcgcccgg
    90661 ttatgcggcg attcctcagg gaaaatccta acgttgaagt tctttttcgc gaaaagatgc
    90721 ccgcgatgca aatggccctg ctggaacgac gcgaactcga tgccgggatc tggcgaatgg
    90781 cgacagaacc accgacaggt tttaccagct tacggttgca tgaatcggcg tttctggtgg
    90841 cgatgcctga agagcatcat ctctcatcat tttccaccgt tccgctggaa gcgctacgtg
    90901 acgagtattt tgttacaatg ccgcccgtgt acactgactg ggattttttg cagcgagttt
    90961 gccagcaggt gggattttca ccggttgtta ttcgcgaagt taatgaaccg caaacggtac
    91021 tcgccatggt cagtatgggt attggtatca cattgatagc ggacagctac gcacaaatga
    91081 actggccagg tgtcattttc cgtccgctca aacagcgcat ccctgcagat ttatatattg
    91141 tttatgaaac acagcaggtg acgcccgcga tggttaagct gttggctgca ttgacacagt
    91201 aggtgaggag acaggaattg ccggaaatag tcgtctgcta agtgaagcgc atcaggcaat
    91261 ttcgcaaatc gcagatagca aaaaagcgcc tttagggcgc ttttttacat tggtgggtcg
    91321 tgcaggatga ctcgcttcgc tcgcccttcg ggccgtcgcc gcgagcggct ccgttgtttc
    91381 gctgcgctca actcgaacct gcggcagctt cgaatctttg agtatcgcag atagcaaaaa
    91441 agcgccttta gggcgctttt tttacattgg tgggtcgtgc aggattcgaa cctgcgacca
    91501 attgattaaa agtcaactgc tctaccaact gagctaacga cccgaagtgg tgggtgatga
    91561 cgggatcgaa ccgccgaccc cctccttgta agggaggtgc tctcccagct gagctaatca
    91621 cccacttcgg tacttcacat ttttacaaga aatttagctg gcgagaaagt ggtgggtgat
    91681 gacgggatcg aaccgccgac cccctccttg taagggaggt gctctcccag ctgagctaat
    91741 cacccacttc tcaatttctt gctacacggc ggagactaca taaagtagtt ggtgggtgat
    91801 gacgggatcg aaccgccgac cccctccttg taagggaggt gctctcccag ctgagctaat
    91861 cacccccgct gtgtggaatc gcattatagg gagagttcaa aatgagtcaa cgcattttct
    91921 aaagaaattg ttcgttcgtc gtaaatttaa gcaagatgat cgcaaaacag accgtgttgc
    91981 gcaatttgtc aacgaaaaca ataatgcgta aggttgcaac ccgaactaca ttgaggaatc
    92041 aggcgggagt gatagaatat cgcccactca atttttccag gatttgccgg ttgtcggcat
    92101 ctttctaaac gtaaggccat ttcatgaaaa tcaaaactcg cttcgcgcca agcccaacag
    92161 gctatctgca cgttggcggc gcgcgtactg ctctttactc ctggcttttt gcacgtaacc
    92221 acggcggtga gttcgtgctg cgtattgaag acaccgatct tgagcgttcc acgccggaag
    92281 ctatcgaagc cattatggat ggcatgaact ggctgagcct ggagtgggat gaaggtccgt
    92341 actaccagac caaacgtttt gatcgctaca atgcggtgat cgatcagatg ctggaagagg
    92401 gcactgctta taaatgctat tgctctaaag agcgcctgga agcgctgcgc gaagagcaaa
    92461 tggcgaaagg tgagaagccg cgttatgacg gtcgctgccg ccacagccat gagcatcatg
    92521 ctgatgatga accgtgtgtc gtgcgttttg ctaatccgca ggaaggttct gttgtttttg
    92581 acgatcagat ccgtggtccg atcgagttca gcaaccagga gctggatgat ctgattatcc
    92641 gccgtaccga tggttcccca acctataact tctgtgtggt tgtcgatgac tgggatatgg
    92701 aaatcaccca cgttatccgt ggcgaagacc atatcaacaa cacgccgcgc cagatcaaca
    92761 ttcttaaggc cctgaaagcg ccggtgccgg tttacgcgca cgtttctatg atcaatggcg
    92821 atgacggtaa aaaactgtcc aaacgtcacg gggcagtcag cgtaatgcag tatcgtgatg
    92881 acggttattt gccagaagca ctgctgaact atctggtgcg tctgggctgg tcccacggcg
    92941 atcaggaaat cttcactcgt gaagagatga tcaaatactt cactttgaat gccgtcagca
    93001 aatctgccag tgcgttcaac accgacaagc tgctgtggct gaaccatcac tacattaacg
    93061 cgctgccgcc ggagtatgtt gctactcact tacagtggca cattgagcag gaaaatatcg
    93121 atacccgtaa cggcccgcag ctggctgatc tggtgaaact gctgggcgaa cgctgcaaga
    93181 cgctgaaaga gatggcacag agctgccgtt atttctacga agattttgct gagttcgatg
    93241 ccgatgcggc gaaaaaacat ctgcgtccgg tagcgcgtca gccgctggaa gtggttcgtg
    93301 acaaactggc cgcgattact gactggaccg ctgaaaacgt tcatcacgct attcaggcga
    93361 cggcggatga gctggaagtg ggtatgggta aagttggtat gccgctgcgt gtcgccgtaa
    93421 ccggtgcggg gcagtctcca gcactggatg ttaccgttca cgcaattggt aagacccgca
    93481 gtatcgagcg tatcaacaaa gcgctggatt ttattgctga acgcgaaaat cagcagtaat
    93541 tagcgtgtaa aaagataaac ggcaggagat aatatcctgc cgttttttat ttatgctgat
    93601 tcgtcaatat cgagacgggc gaggaaattg cgaattcctt cacgtgacaa aaattgcgcg
    93661 actttttcct gctcaatgtc tgtcatatta tctatcgcgt tgatgatttg tcgatacgcc
    93721 ggggcgcgtg ctccaggagc gtattcagca attctctctt cagcttccat caacattggt
    93781 gtgttatgga aggctggcgt gttctgaata aactcgcaaa cttgcgtatc gaccagaata
    93841 agtctggcac gaccgccttt cacgccggga aatttttccg ttttccagcc tttttctctg
    93901 atccaacggt taacagtttg tttagccaca ccgaggcatt cagccagttc ttcggtggtc
    93961 attttgttgc gtaatctttt catattattt gctatcgcga atccctaagc gttgtaacaa
    94021 tccgataatc ccttcccgca gaagcaagga tgtaaactgt ttttgttcaa ctggcgtcat
    94081 ctctttcgcc agtgtcacca gcaatacttc aagcggcgca tcaccagaaa gggcaggcgc
    94141 ttctccctga ccttctggac gttcagcatt gcgaatatat tcacgaacct gttcattgac
    94201 gtgaaccagt cgggctttgc caccctggac gccaggtttt ggtgacgttg tccagccttc
    94261 cttgcgtacc catttattaa tggtctggcg gctatagccg gtcagtctgg caagttcatc
    94321 tggtgtcatc ctctccttga acataacaat tccctgaatg tatgtgggtc tattagtagt
    94381 tcattttata acaccacttt acaggaaacc gtgacatgaa taactactga atgcgagcgg
    94441 ttttccaaaa ttgttcattt gcctgatttt tcagcgattg aaactacacg ttgcaatttg
    94501 ccgttgacac attcgggcgg aattcatatg atgccgcccg tcaactcgac aagctttacg
    94561 tgacggggct atagctcagc tgggagagcg cttgcatggc atgcaagagg tcagcggttc
    94621 gatcccgctt agctccacca aattttgcac ccagcaaatt tggtacgtaa acgcatcgtg
    94681 gggctatagc tcagctggga gagcgcttgc atggcatgca agaggtcagc ggttcgatcc
    94741 cgcttagctc caccaaattt ccaaccctcg ctgcaaagcg ggggtttttt gtctctgctt
    94801 tttgccgctt ttgtaataca gtctacgtcc gggttagcgc cgcctggtga aagcatcatt
    94861 ggatgaaaaa tcggcaacag gctggcccct gtttgcttcg cgatgcgaat aaacttatta
    94921 tttgtgtgcc tgaaaacccc gatcagtgag agtagtgtac tcatgtttgt ggagcataac
    94981 ctgataaaaa atatcaagat attcacacta gcgtttacgc tcaccgtggt acttattcag
    95041 ctatcccgtt ttatttcgcc acttgccatt atccattcca gttatatctt tctggcgtgg
    95101 atgccactgt gcgtaatgct gtcaatcttg tttatctttg gctggcgcgg tgtcgttccc
    95161 gttttatgcg ggatgttttg caccaatctg tggaactttc atctctcttt tttacagact
    95221 gcggtcatgc tcggtagcca gacgtttgtc gtgttgtgtg cctgcgcaat attacgctgg
    95281 cagctgggga cgcgttggcg ttatggattg accagccgat atgtctggca acgtctgttc
    95341 tggcttggtt tggtgacgcc gatcggcatc aaatgcagca tgtatcttgt ggggagcttc
    95401 tttgatttcc cgctaaagat atccaccttt ttcggcgatg cggatgccat tttcacggtc
    95461 gttgatttgc taagcctttt caccgctgtg ctgatttaca acatgctttt ctactatctc
    95521 actcgcatga ttgtaagtcc ccactttgcg cagatattgt ggcgcaggga tatcgctccg
    95581 tcgttgggca aagagaaacg cgcatttacc ttaagctggc tggcagcgtt aagcgtgctg
    95641 ctacttctgt tgtgcacacc ttatgaaaac gactttattg ccggttactt ggtacccgtt
    95701 ttcttcatca tctttaccct cggggtcggt aagcttcgct atccgttttt aaatctcacc
    95761 tgggctgttt caacgctttg cctgctgaat tacaaccaga actttttgca aggggtggaa
    95821 accgaatatt cgctggcatt tattcttgcg gtgctgattt cctttagcgt ttgcctgctc
    95881 tatatggtgc gcatttatca tcgcagtgaa tggcttaatc gccgctggca tttgcaggcg
    95941 ctgacagatc cgttaacgct cctacccaac tttcgtgcgt tggaacaagc gccggagcaa
    96001 gaggcgggca agagtttttg ctgcctgcgc attgataatc ttgagtttat gagtcgtcat
    96061 tacggcttaa tgatgcgcgt tcactgtatc cgctcaattt gccgtacgct gctgccgttg
    96121 atgcaggaaa acgaaaagtt gtatcaattg ccgggtagtg aactgctgtt agtgctgagc
    96181 gggccggaaa cggaagggcg actccagcat atggttaaca tcctgaatag tcggcaaatt
    96241 cactggaaca ataccgggct ggatatgggc tatggtgctg cctgggggcg ttttgatgga
    96301 aatcaggaaa ccctgcaacc cttgttgggg cagttaagct ggctggcgga gcaatcctgc
    96361 gcacatcatc atgtgctggc gctggatagc agagaggaga tggtttccgg gcagaccact
    96421 aaacaggtgc tattgctgaa taccattcgc acggcgttag atcagggtga tttgctgctc
    96481 tacgcccagc caattcgcaa caaagagggt gacggttatg atgagatcct cgcgcgactg
    96541 aaatatgacg gcggcattat gaccccggat aagtttctgc cccttattgc tcagtttaac
    96601 cttagcgcgc gttttgattt gcaagtgctg gaatccttgt tgaagtggct ggcaacacac
    96661 ccttgcgaca aaaaaggacc gcgcttttca gtcaatttaa tgccgctcac gctgctgcaa
    96721 aagaatattg ccgggcggat tattcgtctg tttaagcgtt atcacatctc cccgcaggcg
    96781 gtcattcttg agatcaccga ggagcaggcg ttttctaacg cagaaagcag catgtacaac
    96841 atcgagcagc tgcataagtt tggtttccgg attgcgattg atgactttgg caccggatat
    96901 gccaactacg aacggttaaa gcgtttgcag gctgatatca tcaaaattga tggcgtcttt
    96961 gtgaaagata ttgtcacgaa tacgctggat gcgatgattg tgagatcaat taccgatctg
    97021 gcgaaagcga agtcattgag tgtggtcgcg gagtttgtcg agacgcaaca gcagcaggcg
    97081 ctattgcata agctcggggt gcaatatctg caagggtatt tgattggtcg cccgcagcca
    97141 ttagctgatt aacaggcgta aaaaaaccgg ggaattatcc ccggcttttt tatgtatggt
    97201 cttacagcac cagtgctgcg attgacgcag acagcacact caccagggta gagccgtaaa
    97261 ccagcttcag accgaagcga gaaaccacgt taccttgctc ttcattcagg cctttaactg
    97321 cacctgcgat aatcccgatt gaagagaagt tagcgaagga aaccaggaac acagagatga
    97381 tgccttcagc acgcggagag agcgtggaag caattttctg cagatccatc atcgcaacga
    97441 actcgttgga aaccagtttg gtcgccatga tactgcccac ttgcagtgct tcactggaag
    97501 gaacacccat cacccatgca atcggataga agatgtagcc caggatgccc tggaaggaga
    97561 tgctgtagcc aaaccagcca gtaacggtgg caaacagtgc gttcagcgcg gcgatcaggg
    97621 cgataaagcc gatcagcatc gcggcaacga taatggcaac tttgaaacct gccagaatgt
    97681 attcacccag catttcgaag aagctctgac cttcgtgcag gttggacatc tggatgtttt
    97741 cttcactggc atcaacacgg taaggattga tcagcgacag cacgataaag gtgctgaaca
    97801 tgttcagtac cagcgcagca acgacgtatt tcggttccag catggtcatg tatgcaccaa
    97861 cgatggacat cgacacggtg gacattgccg tggcagccat ggtgtacata cgattacggg
    97921 agattttgcc gaggatatct ttataggcaa taaagttttc agactgaccc agaatcaggg
    97981 agctgacggc gttaaaggat tccagtttgc ccatgccgtt gactttggag agcaggaaac
    98041 caattgcgcg gatgatcacc ggcaacacgc gaatgtgctg gagaataccg atcagtgcag
    98101 agataaagac gattgggcac agcactttca ggaagaagaa tgccaggcct tgatcattca
    98161 tgctaccaaa gacgaagtta gtcccttcgt tggcaaatcc gagcagtttt tcgaacattt
    98221 cggagaagcc tttcacgaag cctaaaccaa cgtcggagtt caggaagaac cacgccagta
    98281 acacttcgat aacaagcagt tgaataacat aacggatacg aatttttttg cggtcgctgc
    98341 ttaccagcag tgcgagaatc gcaacaacgg caagtgccag tacaaaatga aggacgcggt
    98401 ccatatttgc tccaaatatg aggcaggtta aatttccgtg cacattctat gtaacatggt
    98461 taaggaaaac gagatctgac acacactata atgacgtcct gtgacaagct tcaaaaatag
    98521 tgatctgtca tacatttctg ctatgttgtg tatggaagct gaaagttatg taaatgtgct
    98581 aacattaaac ataaccataa cattcaagtc atcaatcttt tcgtagtcat aatcctggtc
    98641 tatcaaagaa atcaccacaa cccattcaaa tgaatttgat aatcattctc gtttggcata
    98701 gcatgaaaca tagcaaaggc tatgttttag aggcacaaga tgacgaacta tcgcgttgag
    98761 agtagcagcg gacgggcggc gcgcaagatg aggctcgcat taatgggacc tgcgttcatt
    98821 gcggcgattg gttatatcga tcccggtaac tttgcgacca atattcaggc gggtgctagc
    98881 ttcggctatc agctactgtg ggttgtcgtt tgggccaacc tgatggcgat gctgattcag
    98941 atcctctctg ccaaactagg gattgccacc ggtaaaaatc tggcggagca gattcgtgat
    99001 cactatccgc gtcccgtagt gtggttctat tgggttcagg cagaaattat tgcgatggca
    99061 accgacctgg cggaatttat tggtgcggcg atcggtttta aactcattct tggtgtttcg
    99121 ttgttgcagg gcgcggtgct gacggggatc gcgactttcc tgattttaat gctgcaacgt
    99181 cgcgggcaaa aaccgctgga gaaagtgatt ggcgggttac tgttgtttgt tgccgcggct
    99241 tacattgtcg agttgatttt ctcccagcct aacctggcgc agctgggtaa aggaatggtg
    99301 atcccgagtt tacctacttc ggaggcggtc ttcctggcag caggcgtgtt aggggcgacg
    99361 attatgccgc atgtgattta tttgcactct tcgctcactc agcatttaca tggcggttcg
    99421 cgtcaacaac gttattccgc caccaaatgg gatgtggcta tcgccatgac gattgccggt
    99481 tttgtcaatc tggcgatgat ggctacagct gcggcggcgt tccacttttc tggtcatact
    99541 ggtgttgccg atcttgatga ggcttatctg acgctgcaac cgttgttaag ccatgctgcg
    99601 gcaacggtct ttggattaag cctggttgct gccggactgt cctcaacggt ggtggggaca
    99661 ctggcagggc aggtggtgat gcaggggttc attcgcttcc atatcccgct gtgggtgcgt
    99721 cgtacagtca ccatgctgcc gtcatttatt gtcattctga tgggattaga tccgacacgg
    99781 attctggtta tgagtcaggt gctgttaagt tttggtatcg ccctggcgct ggttccactg
    99841 ctgattttca ccagtgacag caagttgatg ggcgatctgg tgaacagcaa acgcgtaaaa
    99901 cagacaggct gggtgattgt agtgctggtc gtggcgctaa atatctggtt gttggtgggg
    99961 acggcgctgg gattgtagtt gaatgagcgt cgcatctggc actattggcg gatgcggcgt
   100021 aaacgcctta tccgccctac gcgttaaaga gccggtttgt aggcctgata agacgcgcta
   100081 gcgtcgcatc aggcattgtg ctccattgga ggacgcgttt aatggcggtg cccatgtccg
   100141 cggccttttc cgcgatgatc gtcacgatcg cgccagcctt cacgatagcc acgctcatat
   100201 gctttacgct tatcccagcc acgatgatag ccattatcat gacgccacca gcggtttttg
   100261 cgccattcat aattacgatg ccagtaatca cgatctcgcc agtgaccacc gtcccagtaa
   100321 ttaccgtaat gatcgcgatc gccaatttgt aatttgatcg atggcagtag ggtgatttca
   100381 cctgcatttg ctgcaagcgg ggtaaatgcc atcagggcgg ccgccagaaa cagtgacctg
   100441 aacattgtta ttctccttca cgatcgaagc cgtcagcggc ctgttaacgc aatattacgg
   100501 ggaggtaaag ccccgtatca tcgccataac tcttaaatca taaagaagag cattttttgc
   100561 taaacattat cgtggggtgg ctgttgcttc ggacgattga ccatcatcat cggcttacct
   100621 gctaataaca gccatgccgg aagcatgacg atacagagca gcggtagcaa tgtggtattg
   100681 ggtacaacga ctgccgccat aaaaagactt aaccagccat cgcgtgttac caccagcacg
   100741 atgccgagaa tagcgcagga aacggttatt gccgctggaa cggcgggaac gtgctcatgc
   100801 agcatcaacc ctaatgccac gccgacaaac actgccggga agattcgccc accgcgaaag
   100861 ccactggcgg cagcaacgac cagggcggca agtttaatta ccgccagcaa aaagtaatcg
   100921 ctggtgctga aagcctgatt tgccaccatc tgctgcatct catccagccc tttaaacagc
   100981 gaaaccggcc caccaataac tcccagaata ccgagaataa atccgccaat acctagcacg
   101041 agcaccggat ttttcatttg atgcatcatc gcgtgcaacc gtggtaagca ccatacggcg
   101101 accattcctg ctgcgatggc aatcgccgcg acaattgcac cgctgagaat atcggtcatt
   101161 tccatctgtc cgtaatgcgc aatgggcagt gaaaaatggg gatggaaaaa taatccggta
   101221 gtaagtgcac cagctgctgc cgccattaat ggcgcaaaaa gacgatccca tagcggaact
   101281 tcactactgc catttaaggt ttgcgaaaat atcaacgccg ccgcaacagg tgtaccaaac
   101341 agcgcgccga tggttccggc agaggctaaa atagtccact ccattcggtt gacgcgcggt
   101401 aacagacgag cgccaatggc aaccgcaagg gcgatattga cggtcatgat cggatgttcc
   101461 ggccccaggc tgacgccgcc agcaagaccg agaattaatg ctacgataag tcctggtagc
   101521 gcagagggcg gaactggtgc gccgatcagc ggttcacagg cggggtctgg tccggcatga
   101581 ccctggctga aacggataac caaccccacc gcaatacccg ttagcgttaa tacaccgatg
   101641 atccagagag gtgaatcctg ggctatcccc agagttcccg gcagtcgttg ccagagcaaa
   101701 ttctgtaata ccgaggcgat tttcatcacc acaatcagaa taagactgga cgcaatccca
   101761 attgccacgg cggggagcga taataacaac atggttctgg ctcgcggatg gagcatgttc
   101821 tattccttat gcggggtcag atacttagtt tgcccagctt gcaaaaaggc atcgctgcaa
   101881 ttggtgctga aacgataaag taaatgtgtg acccagatcg atatttacag ggagcctgcc
   101941 tttccggcgt tgttgttatg cccccaggta tttacagtgt gagaaagaat tattttgact
   102001 ttagcggagc agttgaagaa tgacaaagta tgcattagtc ggtgatgtgg gcggcaccaa
   102061 cgcacgtctt gctctgtgtg atattgccag tggtgaaatc tcgcaggcta agacctattc
   102121 agggcttgat taccccagcc tcgaagcggt cattcgcgtt tatcttgaag aacataaggt
   102181 cgaggtgaaa gacggctgta ttgccatcgc ttgcccaatt accggtgact gggtggcaat
   102241 gaccaaccat acctgggcgt tctcaattgc cgaaatgaaa aagaatctcg gttttagcca
   102301 tctggaaatt attaacgatt ttaccgctgt atcgatggcg atcccgatgc tgaaaaaaga
   102361 gcatctgatt cagtttggtg gcgcagaacc ggtcgaaggt aaacctattg cggtttacgg
   102421 tgccggaaca gggcttgggg ttgcgcatct ggtccatgtc gataagcgtt gggtaagctt
   102481 gccgggcgaa ggcggtcacg ttgattttgc gccgaatagt gaagaagagg ccattatcct
   102541 cgaaatactg cgtgcggaaa ttggccatgt ttcggcggaa cgcgtgcttt ctggccctgg
   102601 gctggtgaat ttgtatcgcg caattgtgaa agctgacaac cgcctgccag aaaatctcaa
   102661 gccaaaagat attaccgaac gtgcgctggc tgacagctgc accgattgcc gccgcgcgtt
   102721 gtcgctgttt tgcgtcatta tgggccgttt tggcggcaat ctggcgctca atctcgggac
   102781 atttggcggc gtgtttattg cgggcggtat cgtgccgcgc ttccttgagt tcttcaaagc
   102841 ctctggtttc cgtgccgcat ttgaagataa agggcgcttt aaagaatatg tctatgatat
   102901 tccggtgtat ctcatcgtcc atgacaatcc gggtcttctc ggttccggtg cacatttacg
   102961 ccagacctta ggtcacattc tgtaaatcct tcctgttata tcgggaggta actctcccga
   103021 taatctttta aatcatacag ttaattcaat ttttctttgt gtcccctcac aagaccgacc
   103081 tgcgtcacac ttccgtacag cgggattaat tctccagtaa atgcattatt tgtctggtaa
   103141 cggcgatttg ttttgcacgt tcataatttc actcgtcaac tatcactgaa tgaggaatta
   103201 acgatgagta agaaactaat tgccttatgt gcctgcccaa tgggcctggc tcacactttt
   103261 atggccgctc aggcgctgga agaagcggcg gtagaagccg gttatgaagt gaaaattgaa
   103321 acccagggcg cggacggtat ccagaatcgc ctgacggcgc aggatatcgc cgaagcaacc
   103381 atcatcatcc actccgtggc agttaccccg gaagataatg agcgtttcga atcacgcgac
   103441 gtttatgaaa tcactttgca ggacgcaatt aaaaacgctg cgggcatcat caaagaaatc
   103501 gaagagatga ttgcctctga acaacagtaa tattcgacag ggataattgt tatggccatt
   103561 aaaaaacgca gtgcaaccgt tgtgcctggc gcatccggtg cggcagcggc agttaagaat
   103621 ccgcaggcct ctaaaagcag cttctggggt gaactcccgc agcatgtgat gtcagggatt
   103681 tcacgcatgg tgccgacctt aattatgggc ggtgtgatcc tcgctttcag ccagctgatt
   103741 gcttatagct ggcttaaaat tcctgctgag atcggcatca tggatgctct taatagcggg
   103801 aaattctccg gtttcgacct ctccttactg aaatttgcct ggctgtcgca gtccttcggc
   103861 ggcgtgttgt ttggttttgc cattccgatg tttgctgctt ttgtggcgaa ctctatcggt
   103921 ggcaaactgg cgttcccggc tggttttatc ggcggcttga tgtctaccca gccgacgcaa
   103981 ctgctgaact tcgatcccag cactatgcaa tgggcgacct cttcgccggt gccgtccacc
   104041 ttcattggcg cgctgattat ttctatcgtt gccggttacc ttgtgaaatg gatgaaccag
   104101 aaaatccagt taccggattt cctgttagcg ttcaaaacca catttttgct accgattctt
   104161 tccgccattt ttgtcatgct ggcgatgtac tacgtcatca ccccgtttgg cggctggatc
   104221 aacggcggta tccgtactgt gctgactgcc gcaggtgaga agggcgcgct gatgtacgca
   104281 atggggatcg ccgcggcgac agcaatcgac cttggtggcc cgatcaacaa agccgcaggt
   104341 ttcgttgcct tcagctttac cactgaccac gtattaccgg tcaccgctcg ttctatcgct
   104401 atcgttattc ctccgattgg tctgggtctg gcgaccatta ttgaccgtcg tttaaccggc
   104461 aaaggcctgt tcaacgctca actttatccg cagggtaaga ccgcgatgtt ccttgccttt
   104521 atggggatta gtgagggtgc gattccgttt gcgctggaaa gccccatcac cgccattccg
   104581 tcgtatatgg tcggcgcgat tgtcggctca accgccgctg tctggctggg cgcagtgcaa
   104641 tggttcccgg aatccgctat ctgggcatgg ccgctggtca ctaacctggg cgtctatatg
   104701 gcggggatcg cgctgggggc agtcataact gcgctgatgg tggtgttcct gcgtctgatg
   104761 atgttccgta aaggcaaatt gttaatcgat agcctgtaag aaggaaaatc cagatgacat
   104821 tactcgcttc gctgcgcgac tggcttaagg cgcaacaact ggatgcagtg cttctctcct
   104881 cacggcagaa caaacagccg catctgggga tctccaccgg atcaggctat gtggttatta
   104941 gccgtgaaag tgcgcacatt ctggtagatt cgcgctattt cgttgaggtg gaagcccgtg
   105001 cgcaaggcta ccagctgcat ttgcttgacg cgacgaacac gcttaccact atcgtcaatc
   105061 aaatcattgc cgatgagcag ttgcaaacgc tcggttttga aggccagcag gtgagttggg
   105121 aaaccgcgca ccgctggaag tctgaactca atgcgaaact ggttagcgcc acgccggatg
   105181 tgctgcggca aatcaaaacg ccagaggagg tggagataat ccgcctcgcc tgtgggattg
   105241 ccgatcgcgg agcggagcat attcgccgct ttattcaggc ggggatgagc gagcgcgaga
   105301 tagccgctga actggagtgg tttatgcgcc agcagggcgc agaaaaagcc tcttttgaca
   105361 ccattgtcgc cagcggctgg cgcggggcgc tgccgcacgg caaagccagc gacaagattg
   105421 ttgcagcggg cgagtttgtc actctcgatt ttggtgcgct gtatcagggc tactgctctg
   105481 atatgacgcg caccttgctg gtgaatggcg aaggggtgag cgccgaatct cacccgctgt
   105541 ttaacgtcta ccagattgtc ctgcaggcac agctcgcagc aatctctgca attcgccccg
   105601 gcgtgcgctg ccagcaggtt gacgatgccg cgcgccaagt gattaccgag gcaggtttta
   105661 gccactattt cggtcataac accggtcatg ctatcggcat tgaagttcat gaagatccgc
   105721 gtttttcacc gcgggacacc acgacgctac agccaggcat gttactgacc gtggagccgg
   105781 ggatttattt gccagggcaa gggggcgtgc gcatcgaaga tgttgtgctg gttaccccgc
   105841 aaggcgcaga agtgctctac gccatgccga aaacagtgtt gctcacggga gaggcataat
   105901 ggatttatcg ctattaaaag cgttgagcga ggcagatgcg atcgcctcct cggaacagga
   105961 agtgcggcag atcctgctgg aagaagcgga tcgcctgcaa aaagaagtgc gatttgatgg
   106021 tctgggatcg gtgctgatcc gcctcaatga atcgacaggt ccgaaggtga tgatctgcgc
   106081 ccatatggac gaagtgggat ttatggtgcg tagcatctcc cgcgaagggg caattgatgt
   106141 gctgccggtt ggcaacgtgc gcatggctgc ccgccagctg cagccggtgc gcatcaccac
   106201 ccgtgaagag tgcaaaattc caggcctgct tgacggcgac cggcagggga atgacgtcag
   106261 cgccatgcgc gtggacattg gtgcgcgctc gtatgacgaa gtgatgcagg cgggaattcg
   106321 tccaggcgat cgcgtcacct ttgatacaac ttttcaggtt ctccctcacc agcgggtgat
   106381 ggggaaagcc tttgatgatc gcctcggttg ctatctgctg gtgacgttac tacgtgagct
   106441 gcacgatgcc gaactgcctg cggaggtgtg gctggtggca agttccagcg aagaggtggg
   106501 attacgcggt gggcaaaccg ctacccgcgc ggtgtcgccg gacgtcgcca ttgtgctgga
   106561 taccgcctgc tgggcgaaaa actttgatta tggcgcggct aaccatcgcc agattggtaa
   106621 cggcccgatg ctggtgttaa gcgacaagtc actgattgcg ccgccaaaac ttaccgcctg
   106681 gatcgaaacc gtggcggctg aaattggcgt accgttgcag gcagatatgt tcagcaacgg
   106741 cggcacggac ggcggagcgg tgcacttaac cggtactggc gtacccacag tggtaatggg
   106801 gcctgccacc cgccacggac attgcgcggc atcgattgcc gattgccgcg acattttgca
   106861 gatggagcaa cttttatctg cccttattca acgtcttacg cgtgagacgg ttgttcaact
   106921 gacggatttc agatgatctc ctgattaacc cggagcggtt atgttaacga ttcaatttct
   106981 ctgtcctttg cctaacggtc tacatgctcg tccggcgtgg gaacttaaag aacagtgcag
   107041 ccagtggcaa agcgaaatca cctttttcaa ccatcgccag aacgcaaagg ccgatgctaa
   107101 aagctcgctg gcgttgattg gaactggcac cttatttaat gacagttgca gcctgaacat
   107161 tagcggcagc gatgaagagc aggcgcggcg cgtgctggaa gagtacatcc tggtgcgctt
   107221 tatcgacagc gacagcgttc agccgacgca ggcagaactg acggcgcatc cgctgccgcg
   107281 ttcattaagc cgccttaacc cggatttact gtacggcaat gtgctggcaa gcggcgtcgg
   107341 cgtgggtacg ctgaccctgt tacaaagcga cagcctcgac agttatcggg caatccccgc
   107401 cagtgcgcaa gattccaccc ggctggagca cagcctggca acgcttgccg agcaactgaa
   107461 ccagcaattg cgtgagcgtg acggcgaaag caaaactatc ctcagcgccc atttgtcgct
   107521 gattcaggat gatgaattcg cgggcaatat ccgtcgtctg atgacagaac agcatcaagg
   107581 gctgggggcg gcgatcatca gcaatatgga gcaggtttgc gccaaacttt ctgcttctgc
   107641 cagtgattat ctgcgcgaac gtgttagcga cattcgcgat attagcgaac agttgctgca
   107701 tatcacctgg ccggaactga agccgcgcaa caatctggtg ctggaaaaac cgactattct
   107761 ggtagccgag gatttaaccc caagccagtt tttgagcctc gatttaaaaa atcttgcagg
   107821 catgattctg gagaaaactg ggcgcacctc gcataccctg attctgaccc gcgcctcggc
   107881 gatcccggta ctgagcggcc tgccgctgga tgcgattgcc cgttatgccg ggcaaccggc
   107941 ggtgcttgac gcccagtgcg gcgtgctggc gattaacccg aatgacgcgg tgagcggtta
   108001 ttatcaggtc gcacagacgc tggcggataa acgccaaaaa caacaggcgc aggccgccgc
   108061 gcagctggcc tattcccgtg ataacaagcg tattgatatt gcggcgaata tcggcaccgc
   108121 tctggaagcg ccaggcgcgt ttgccaacgg cgcggaaggc gttggactgt tccgtactga
   108181 aatgctctat atggatcgcg acagcgcgcc ggacgagcag gagcaatttg aagcctacca
   108241 gcaggtgcta ctggcagcgg gcgacaagcc gattatcttc cgcacaatgg acatcggtgg
   108301 cgataaaagc attccttatc tgaatattcc ccaggaagag aacccgttcc tcggctatcg
   108361 cgcggtacgt atttacccgg aatttgctgg cctgttccgc actcaactac gggcgatttt
   108421 gcgcgccgcc agttttggca acgcccagtt gatgatcccg atggtccaca gtctcgatca
   108481 gatcttatgg gtgaagggcg agatccaaaa agcgatcgtt gagcttaagc gcgatggcct
   108541 gcgtcatgca gagacgatta cgctggggat catggtggaa gtgccgtcgg tgtgctacat
   108601 catcgatcac ttctgcgatg aggtcgattt cttcagtatc ggctccaacg atatgaccca
   108661 gtatctgtat gcggtcgatc gtaataaccc gcgcgtatcg ccgctgtata acccgattac
   108721 gccatcgttc ctgcgcatgt tgcagcagat tgttaccact gcgcatcagc ggggcaaatg
   108781 ggtaggcatt tgcggtgaac tgggcggtga aagccgttat ctgccgctac tgcttgggct
   108841 gggcctggat gagctgagta tgagtagccc gcgtattccg gcggtgaaaa gccagcttcg
   108901 tcaactggat agcgaggcgt gtcgggaact ggcgcgtcag gcatgtgaat gccgcagtgc
   108961 gcaggaaatt gaagcgttac tcaccgcctt tacgccggaa gaagacgttc gcccactgct
   109021 ggcgctggag aatatctttg ttgatcagga ttttagcaat aaagagcagg cgatccagtt
   109081 cctgtgcggc aacctcggcg ttaacgggcg cactgaacat ccgtttgagc tggaagaaga
   109141 tgtctggcag cgggaagaga ttgtcaccac cggcgttggt tttggcgtag cgatcccgca
   109201 caccaaatct cagtggatcc gtcattccag tatcagcatt gcccggctgg cgaaaccggt
   109261 tgactggcag tcagaaatgg gcgaagtcga actggtgatc atgctgacgc tgggtgctaa
   109321 cgaagggatg aatcatgtga aagtcttctc gcagctggcg cgtaaactgg tgaataaaaa
   109381 cttccgccag tcgttgtttg ccgcgcaaga tgcacaaagt atcctgacgc tgctggaaac
   109441 agaattaacc ttctgacgtt agccctgaaa acgggcgctg tactctcccg gcgtcagacc
   109501 aaactgacgc cggaaaacgc gacaaaaata gtcgctatcc ggaaaaccgc aacgctgtgc
   109561 cacttcatga attgacagat gatatttctg caaaatcatc ctcgccttcg ccattcgcac
   109621 ccaacgcaca tactcgataa aacgcatcgt tccatgctga gcaaacagtt ttgacagatg
   109681 attgggcgtg atattaaaaa actgcgccac gctctcgcgg gtgagcggct gggcataatt
   109741 atcctgaacc cagttacaaa tgctgtgata gagaaaatcg ccgcgcggtt gcgaattacc
   109801 cggcggcata ttcaccacgt tgcggcaaag atgcagcaaa cttaacacca gtggctgaat
   109861 gatattctgc tccagcggcg aacggctaag atgggtgagg gcggtaagca tcgcttcacc
   109921 ttcaccgcgt tgcaggctgg gaagctggat ctggcgagca ggatgcaaca acgacgtggt
   109981 gcggctatcg taaaacgata acccaagcca ggtcggtgcg aacaccaggc ttaacagcat
   110041 caccggtttg ttgttgatcg gtaaattagc cgcgcgtgcc ggaataaaca gcatttctcc
   110101 ttccaccatt cgctgctgct ttgcttccag caggttgccg tattcgccgc gcagcacgat
   110161 atccagacgg ggaaaatcaa tacataaact gcccaccggt aacgaggcag gctggctggc
   110221 aaaccagacg cgccccagta gttgcgggtt aagcaccagg ccgctgaaca gatcggcgaa
   110281 aaattgttga tcggcgggca agcctggtgc tttcatgaaa gttcccgatt aaagatgcat
   110341 taactggcga aattctttca ctttgctgcg actgaccggc acttcaaaat ccagatcttt
   110401 cagtcgcaga atgtaggtgt tattaaacca cggttcgatt tcgcgtattt tgttcagatt
   110461 gacacaaaat gagcgatggc agcggaaaaa atgcgacggc ggcagtttgc tgcaaaattc
   110521 ggtaatgttc atcggcatta cgtaggattc acgccgcgta tagacaaagg tcattttctc
   110581 gtgcgcttcg gcgtaataga tatcgttaat tggcgtgacg attattcgct catctttcac
   110641 cagattaatg gtgtcattct cacgcgttac cgtcgcggca ggcgtactgc tggtctgctg
   110701 ttgttgccag gccgcttcca gtttttgcag catcccggta atacgtgact cctgatacgg
   110761 tttgagaatg tagtcaaacg cctccagttc aaacgcttct accgcatgtt ctttccacgc
   110821 ggtgatgaac acaataaacg gtttattggc gaactggctg atgttttgcg ccagcaacac
   110881 gccatccagc gacggaatat tgatatccag aaaaatggcg tcgacgcggt tatgctgcaa
   110941 aaacttcaac acgtccagac cgtcgtcaaa ggtgccgaca atctccatct ggctgtgctc
   111001 tttaattagc cagctcagtt cctgttgtgc caggaattcg tcttcaacaa tgatgacttt
   111061 cacaatatca ctccggctca aagcaataac gtagcctgtg aggcgactgg ggtgcgttgg
   111121 ttaggaatgt aaaacgcaat ttccgtcccc ggctccaggc ggcggatatg cagcccctcg
   111181 ccatacaata acttcacgcg atgatggaca ttcagcaggc caattttatt gcccggcatt
   111241 tcattcgctt cgacccgctc aatcaccttt ggatcgatgc cgtgcccggt atctcgcacc
   111301 gcaatgcgta cccgatttcc gcactctgca acgctgatgg tgacaacgcc tttacctttg
   111361 caaggctgaa taccgtggac aatggcgttc tccaccaacg gctggatcag caggctggga
   111421 atgcagcaat tcacctcttc atcaatatca tagataaccg tcagcttgtc accaaagcgg
   111481 gcctgctcaa tggcaatata gtctttaatt tgatacagct cttttttgat atcgatttgc
   111541 tcatcgtctt ttaattcaat gttatagcgc agataacgcg acagattaaa gatcaactgg
   111601 cgagcggtat ccggattcag acggattgac gatgaaatag cgttcagagc gttaaacaga
   111661 aaatggggat taattttgct ttgcagggcg cgaagctctg ccttatttgc catttcacgt
   111721 agctgctcgg cgcgtgaaac ctccagttgc gtggagatga tttgcgacag accgaccgcc
   111781 atttcctgta atgacgaggt gatctgatgc gcgtggcagt agtaaatttt cagcgttccg
   111841 gtcacgaccc ctttctccca caatgggatc accagcatgg aatgaatctc tggtgtgcgg
   111901 tgggcttcat cattgttttt aatgatgatt tttccgtaat tcatcgcctg acgggtagtc
   111961 gggctaatga agtcatcgcc attctgatag ttatgttcac ccacgccaac ataggccagc
   112021 acatgatcgg tattagtaat cgccaccgca tcggcgtgaa tgtcatcgcg gataatttcg
   112081 cagaccttgc gtaatgactc gctattgaca tggcgaaaca gcggtagcgt tttgttggcg
   112141 atatccagcg ccagcttggc ctgccgcgcg gcgctggcct ctttttcgcc ctcaacgctt
   112201 tgcaccagaa gcacaataaa gccgatacag acgctaccga ggatcattgg aatgccgatt
   112261 ttagagacga tatcgatccc caacgcggta gttggtgccc agacaattac cagaatcatg
   112321 gtcaacgtct cacacaacat gccgccgaga ataccgacgc gccagcgctg tgctttgggg
   112381 attttcaggt tgatccagcc cgatatacaa cccgccagaa tgctggtgat aaagcagggg
   112441 atcgccgtca cgccgccgat atcaattaaa taccggtgaa tacccgcaat cacgccggta
   112501 atgatgccta cccacgggcc aaacagaatc ccgccggaca tcaccgcgat aatacgtacg
   112561 ttcaccagcg agccttctac gggaacgccg gaccaggtgc tgaacagggc gaacagcgag
   112621 aaaatggcgg taacggcgag caattctttt ggggagtgcg ccgacttgtg caacagttcg
   112681 cgaaacaggc ggatacggat gagaaagaac aggcagataa gcattaacgc tgcccgatcg
   112741 aagaccgcca gcagcatgtt gaatatttcg tgcacgggtg actctgaata aggattaaac
   112801 ctctatgata ggtaacctga aggctgatga ccagcaggcc gtttttgagg aaaaaccata
   112861 aatttaaaag gggattgaaa gataaatggt gtattagcaa tgtatctctt gataatgcac
   112921 ttctggttgg taaatttatt gaaattataa tgttaataaa attggtggta attttcttgt
   112981 tagcgtagaa aaatttcctg tttttgaagt
//
LOCUS       DAZGJW010000002        83590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZGJW010000002 DAZGJW010000000
VERSION     DAZGJW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 83590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.002"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..352
                     /locus_tag="ACHKIK_000114"
     CDS             <1..352
                     /locus_tag="ACHKIK_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO9081357.1"
                     /translation="GTEIAITYVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPL
                     SAKYKNIAGVEEKLTYTDTYAQENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQ
                     MELVMKAAGFKEVK"
     gene            complement(392..862)
                     /gene="yehS"
                     /locus_tag="ACHKIK_000115"
     CDS             complement(392..862)
                     /gene="yehS"
                     /locus_tag="ACHKIK_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO9081358.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIFLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(909..1628)
                     /gene="btsR"
                     /locus_tag="ACHKIK_000116"
                     /gene_synonym="yehT"
     CDS             complement(909..1628)
                     /gene="btsR"
                     /locus_tag="ACHKIK_000116"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO9081359.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1625..3310)
                     /gene="btsS"
                     /locus_tag="ACHKIK_000117"
     CDS             complement(1625..3310)
                     /gene="btsS"
                     /locus_tag="ACHKIK_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO9081360.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3532..4263
                     /gene="mlrA"
                     /locus_tag="ACHKIK_000118"
     CDS             3532..4263
                     /gene="mlrA"
                     /locus_tag="ACHKIK_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO9081361.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            4323..4430
                     /gene="yohO"
                     /locus_tag="ACHKIK_000119"
     CDS             4323..4430
                     /gene="yohO"
                     /locus_tag="ACHKIK_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO9081362.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(4411..5142)
                     /gene="yehW"
                     /locus_tag="ACHKIK_000120"
     CDS             complement(4411..5142)
                     /gene="yehW"
                     /locus_tag="ACHKIK_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO9081363.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(5147..6073)
                     /gene="yehX"
                     /locus_tag="ACHKIK_000121"
     CDS             complement(5147..6073)
                     /gene="yehX"
                     /locus_tag="ACHKIK_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO9081364.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(6066..7223)
                     /gene="yehY"
                     /locus_tag="ACHKIK_000122"
     CDS             complement(6066..7223)
                     /gene="yehY"
                     /locus_tag="ACHKIK_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO9081365.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(7230..8147)
                     /gene="osmF"
                     /locus_tag="ACHKIK_000123"
     CDS             complement(7230..8147)
                     /gene="osmF"
                     /locus_tag="ACHKIK_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO9081366.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGSVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(8358..10655)
                     /gene="bglX"
                     /locus_tag="ACHKIK_000124"
     CDS             complement(8358..10655)
                     /gene="bglX"
                     /locus_tag="ACHKIK_000124"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO9081367.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            10851..12566
                     /gene="dld"
                     /locus_tag="ACHKIK_000125"
     CDS             10851..12566
                     /gene="dld"
                     /locus_tag="ACHKIK_000125"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO9081368.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(12604..13536)
                     /gene="pbpG"
                     /locus_tag="ACHKIK_000126"
     CDS             complement(12604..13536)
                     /gene="pbpG"
                     /locus_tag="ACHKIK_000126"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO9081369.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(13710..14297)
                     /gene="yohC"
                     /locus_tag="ACHKIK_000127"
     CDS             complement(13710..14297)
                     /gene="yohC"
                     /locus_tag="ACHKIK_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO9081370.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            14467..15045
                     /gene="yohD"
                     /locus_tag="ACHKIK_000128"
     CDS             14467..15045
                     /gene="yohD"
                     /locus_tag="ACHKIK_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO9081371.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(15175..15936)
                     /gene="yohF"
                     /locus_tag="ACHKIK_000129"
     CDS             complement(15175..15936)
                     /gene="yohF"
                     /locus_tag="ACHKIK_000129"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9081372.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATYEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(15989..17425)
                     /gene="mdtQ"
                     /locus_tag="ACHKIK_000130"
     CDS             complement(15989..17425)
                     /gene="mdtQ"
                     /locus_tag="ACHKIK_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO9081373.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADVQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            17649..17732
                     /gene="yohP"
                     /locus_tag="ACHKIK_000131"
     CDS             17649..17732
                     /gene="yohP"
                     /locus_tag="ACHKIK_000131"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO9081374.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(18120..19070)
                     /gene="dusC"
                     /locus_tag="ACHKIK_000132"
     CDS             complement(18120..19070)
                     /gene="dusC"
                     /locus_tag="ACHKIK_000132"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO9081375.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            19309..19707
                     /gene="yohJ"
                     /locus_tag="ACHKIK_000133"
     CDS             19309..19707
                     /gene="yohJ"
                     /locus_tag="ACHKIK_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO9081376.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            19704..20399
                     /gene="yohK"
                     /locus_tag="ACHKIK_000134"
     CDS             19704..20399
                     /gene="yohK"
                     /locus_tag="ACHKIK_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO9081377.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            20529..21413
                     /gene="cdd"
                     /locus_tag="ACHKIK_000135"
     CDS             20529..21413
                     /gene="cdd"
                     /locus_tag="ACHKIK_000135"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO9081378.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            21563..22282
                     /gene="sanA"
                     /locus_tag="ACHKIK_000136"
     CDS             21563..22282
                     /gene="sanA"
                     /locus_tag="ACHKIK_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO9081379.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            22285..22524
                     /gene="yeiS"
                     /locus_tag="ACHKIK_000137"
     CDS             22285..22524
                     /gene="yeiS"
                     /locus_tag="ACHKIK_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO9081380.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            22843..24081
                     /gene="preT"
                     /locus_tag="ACHKIK_000138"
     CDS             22843..24081
                     /gene="preT"
                     /locus_tag="ACHKIK_000138"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO9081381.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            24075..25310
                     /gene="preA"
                     /locus_tag="ACHKIK_000139"
     CDS             24075..25310
                     /gene="preA"
                     /locus_tag="ACHKIK_000139"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO9081382.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(25381..26391)
                     /gene="mglC"
                     /locus_tag="ACHKIK_000140"
     CDS             complement(25381..26391)
                     /gene="mglC"
                     /locus_tag="ACHKIK_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO9081383.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(26407..27927)
                     /gene="mglA"
                     /locus_tag="ACHKIK_000141"
     CDS             complement(26407..27927)
                     /gene="mglA"
                     /locus_tag="ACHKIK_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO9081384.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDISFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(27988..28986)
                     /gene="mglB"
                     /locus_tag="ACHKIK_000142"
     CDS             complement(27988..28986)
                     /gene="mglB"
                     /locus_tag="ACHKIK_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO9081385.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(29266..30306)
                     /gene="galS"
                     /locus_tag="ACHKIK_000143"
     CDS             complement(29266..30306)
                     /gene="galS"
                     /locus_tag="ACHKIK_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO9081386.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            complement(30448..31605)
                     /gene="yeiB"
                     /locus_tag="ACHKIK_000144"
     CDS             complement(30448..31605)
                     /gene="yeiB"
                     /locus_tag="ACHKIK_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO9081387.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIAQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(31622..32290)
                     /gene="folE"
                     /locus_tag="ACHKIK_000145"
     CDS             complement(31622..32290)
                     /gene="folE"
                     /locus_tag="ACHKIK_000145"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO9081388.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            32548..33384
                     /gene="yeiG"
                     /locus_tag="ACHKIK_000146"
     CDS             32548..33384
                     /gene="yeiG"
                     /locus_tag="ACHKIK_000146"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO9081389.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTIKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(33416..35407)
                     /gene="cirA"
                     /locus_tag="ACHKIK_000147"
     CDS             complement(33416..35407)
                     /gene="cirA"
                     /locus_tag="ACHKIK_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO9081390.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(35700..37169)
                     /gene="lysP"
                     /locus_tag="ACHKIK_000148"
     CDS             complement(35700..37169)
                     /gene="lysP"
                     /locus_tag="ACHKIK_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO9081391.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(37374..38255)
                     /gene="yieE"
                     /locus_tag="ACHKIK_000149"
     CDS             complement(37374..38255)
                     /gene="yieE"
                     /locus_tag="ACHKIK_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO9081392.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIKDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            38354..39403
                     /gene="yeiH"
                     /locus_tag="ACHKIK_000150"
     CDS             38354..39403
                     /gene="yeiH"
                     /locus_tag="ACHKIK_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO9081393.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            39477..40334
                     /gene="nfo"
                     /locus_tag="ACHKIK_000151"
     CDS             39477..40334
                     /gene="nfo"
                     /locus_tag="ACHKIK_000151"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO9081394.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            40338..41426
                     /locus_tag="ACHKIK_000152"
     CDS             40338..41426
                     /locus_tag="ACHKIK_000152"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO9081395.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(41482..42732)
                     /gene="nupX"
                     /locus_tag="ACHKIK_000153"
     CDS             complement(41482..42732)
                     /gene="nupX"
                     /locus_tag="ACHKIK_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO9081396.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(42832..43773)
                     /gene="rihB"
                     /locus_tag="ACHKIK_000154"
     CDS             complement(42832..43773)
                     /gene="rihB"
                     /locus_tag="ACHKIK_000154"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO9081397.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            43903..44601
                     /gene="yeiL"
                     /locus_tag="ACHKIK_000155"
     CDS             43903..44601
                     /gene="yeiL"
                     /locus_tag="ACHKIK_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO9081398.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(44669..45919)
                     /locus_tag="ACHKIK_000156"
     CDS             complement(44669..45919)
                     /locus_tag="ACHKIK_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO9081399.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(46013..46951)
                     /gene="psuG"
                     /locus_tag="ACHKIK_000157"
     CDS             complement(46013..46951)
                     /gene="psuG"
                     /locus_tag="ACHKIK_000157"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO9081400.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(46939..47880)
                     /gene="psuK"
                     /locus_tag="ACHKIK_000158"
     CDS             complement(46939..47880)
                     /gene="psuK"
                     /locus_tag="ACHKIK_000158"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO9081401.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRGDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            48065..48211
                     /locus_tag="ACHKIK_000159"
     CDS             48065..48211
                     /locus_tag="ACHKIK_000159"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081402.1"
                     /translation="MPELQKRGICDGMHRTGTLKKGRKESALEEITLVFDERFRFLLK
                     DKTI"
     gene            complement(48304..49995)
                     /gene="fruA"
                     /locus_tag="ACHKIK_000160"
     CDS             complement(48304..49995)
                     /gene="fruA"
                     /locus_tag="ACHKIK_000160"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO9081403.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(50012..50950)
                     /gene="fruK"
                     /locus_tag="ACHKIK_000161"
     CDS             complement(50012..50950)
                     /gene="fruK"
                     /locus_tag="ACHKIK_000161"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO9081404.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(50950..52080)
                     /gene="fruB"
                     /locus_tag="ACHKIK_000162"
     CDS             complement(50950..52080)
                     /gene="fruB"
                     /locus_tag="ACHKIK_000162"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO9081405.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQLAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFTEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIA
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            52448..53629
                     /gene="setB"
                     /locus_tag="ACHKIK_000163"
     CDS             52448..53629
                     /gene="setB"
                     /locus_tag="ACHKIK_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO9081406.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(53626..53880)
                     /gene="yeiW"
                     /locus_tag="ACHKIK_000164"
     CDS             complement(53626..53880)
                     /gene="yeiW"
                     /locus_tag="ACHKIK_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO9081407.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            54035..54607
                     /gene="yeiP"
                     /locus_tag="ACHKIK_000165"
     CDS             54035..54607
                     /gene="yeiP"
                     /locus_tag="ACHKIK_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO9081408.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            54830..56296
                     /gene="yeiQ"
                     /locus_tag="ACHKIK_000166"
     CDS             54830..56296
                     /gene="yeiQ"
                     /locus_tag="ACHKIK_000166"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO9081409.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLVEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHQGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            56414..57400
                     /gene="yeiR"
                     /locus_tag="ACHKIK_000167"
     CDS             56414..57400
                     /gene="yeiR"
                     /locus_tag="ACHKIK_000167"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO9081410.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            57439..58152
                     /gene="lpxT"
                     /locus_tag="ACHKIK_000168"
     CDS             57439..58152
                     /gene="lpxT"
                     /locus_tag="ACHKIK_000168"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO9081411.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            58564..59130
                     /gene="mepS"
                     /locus_tag="ACHKIK_000169"
     CDS             58564..59130
                     /gene="mepS"
                     /locus_tag="ACHKIK_000169"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO9081412.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            59311..60867
                     /gene="pdeN"
                     /locus_tag="ACHKIK_000170"
     CDS             59311..60867
                     /gene="pdeN"
                     /locus_tag="ACHKIK_000170"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO9081413.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            60949..62763
                     /gene="yejA"
                     /locus_tag="ACHKIK_000171"
     CDS             60949..62763
                     /gene="yejA"
                     /locus_tag="ACHKIK_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO9081414.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDTFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            62764..63858
                     /gene="yejB"
                     /locus_tag="ACHKIK_000172"
     CDS             62764..63858
                     /gene="yejB"
                     /locus_tag="ACHKIK_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO9081415.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSKYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            63858..64883
                     /gene="yejE"
                     /locus_tag="ACHKIK_000173"
     CDS             63858..64883
                     /gene="yejE"
                     /locus_tag="ACHKIK_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9081416.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            64885..66474
                     /gene="yejF"
                     /locus_tag="ACHKIK_000174"
     CDS             64885..66474
                     /gene="yejF"
                     /locus_tag="ACHKIK_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO9081417.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(66478..66822)
                     /gene="yejG"
                     /locus_tag="ACHKIK_000175"
     CDS             complement(66478..66822)
                     /gene="yejG"
                     /locus_tag="ACHKIK_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO9081418.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(67155..68345)
                     /gene="bcr"
                     /locus_tag="ACHKIK_000176"
     CDS             complement(67155..68345)
                     /gene="bcr"
                     /locus_tag="ACHKIK_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO9081419.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            complement(68373..69068)
                     /gene="rsuA"
                     /locus_tag="ACHKIK_000177"
     CDS             complement(68373..69068)
                     /gene="rsuA"
                     /locus_tag="ACHKIK_000177"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO9081420.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            69217..70977
                     /gene="radD"
                     /locus_tag="ACHKIK_000178"
     CDS             69217..70977
                     /gene="radD"
                     /locus_tag="ACHKIK_000178"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO9081421.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGNEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            71102..71386
                     /gene="rplY"
                     /locus_tag="ACHKIK_000179"
     CDS             71102..71386
                     /gene="rplY"
                     /locus_tag="ACHKIK_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO9081422.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(71525..72532)
                     /gene="yejK"
                     /locus_tag="ACHKIK_000180"
     CDS             complement(71525..72532)
                     /gene="yejK"
                     /locus_tag="ACHKIK_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO9081423.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            72714..72941
                     /gene="yejL"
                     /locus_tag="ACHKIK_000181"
     CDS             72714..72941
                     /gene="yejL"
                     /locus_tag="ACHKIK_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO9081424.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            72961..74721
                     /gene="yejM"
                     /locus_tag="ACHKIK_000182"
     CDS             72961..74721
                     /gene="yejM"
                     /locus_tag="ACHKIK_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO9081425.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            74796..74872
                     /locus_tag="ACHKIK_000183"
     tRNA            74796..74872
                     /locus_tag="ACHKIK_000183"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74830..74832,aa:Pro,seq:ggg)
     gene            75091..76332
                     /locus_tag="ACHKIK_000184"
     CDS             75091..76332
                     /locus_tag="ACHKIK_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO9081426.1"
                     /translation="MARKTHPLTTVQIKAARPAQKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLEDVSLADARKRRSEYRTLISAGTDPQDHERQKRETEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            76431..76661
                     /locus_tag="ACHKIK_000185"
     CDS             76431..76661
                     /locus_tag="ACHKIK_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081427.1"
                     /translation="MQKNIRCNCDGLQLALMVQHEFWSTYDPEDRTTAPSKKQVVDFL
                     VSRGASRNLAVSIDKVVRPASMKIGGRPKKWR"
     gene            76829..77035
                     /locus_tag="ACHKIK_000186"
     CDS             76829..77035
                     /locus_tag="ACHKIK_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9081428.1"
                     /translation="MEAIRKIIFRQEVKKIIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVLSWQKEREEEARGTAA"
     gene            77050..77526
                     /locus_tag="ACHKIK_000187"
                     /pseudo
     CDS             77050..77526
                     /locus_tag="ACHKIK_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            77719..78057
                     /locus_tag="ACHKIK_000188"
                     /pseudo
     CDS             77719..78057
                     /locus_tag="ACHKIK_000188"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            78088..78276
                     /locus_tag="ACHKIK_000189"
     CDS             78088..78276
                     /locus_tag="ACHKIK_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO9081429.1"
                     /translation="MATIPTLVHSQTAFLWRFIIFGASEHQIIHVTAWTEREARSRCP
                     SGCVAVFAARIRQGSHHA"
     gene            78269..78733
                     /locus_tag="ACHKIK_000190"
     CDS             78269..78733
                     /locus_tag="ACHKIK_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO9081430.1"
                     /translation="MRKNRLQKIITGLYASPVAHMATGFSSLNTVADSRLHSDMRFFC
                     VRKPTHTRIMAGRNGGAFALAGSYSTSLLTLLRLATPFSSVVARLLKITVGAFHMAVS
                     ARPYFVWRFMQCHHSNIDVFTVTAATEREARAQLPHAHLIFVARIRQGVSHA"
     gene            78726..78953
                     /locus_tag="ACHKIK_000191"
     CDS             78726..78953
                     /locus_tag="ACHKIK_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081431.1"
                     /translation="MHKIPYDVLIHSENALIKAREMNALLIKLIDAQEDGGEPASLIF
                     AAVQTLLSPVIDELDKAMAIHENNKAPHTGE"
     gene            78959..79258
                     /locus_tag="ACHKIK_000192"
     CDS             78959..79258
                     /locus_tag="ACHKIK_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9081432.1"
                     /translation="MKLKYSGLTASGKTRTKFMCGDIYRDKYGGTVMIKGVDERRVTY
                     RREGYEYDCVMPVYQFDRDFYLVQAAPRSKPTSSEKARANIQEIKKMLNVFRGKK"
     gene            79255..80664
                     /locus_tag="ACHKIK_000193"
     CDS             79255..80664
                     /locus_tag="ACHKIK_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO9081433.1"
                     /translation="MKLAPNVKRLPKDKYTEAIIFAGNDAHSFAEHYIIAQAKKAGDP
                     VPPVYLGCYQLSELDNLQIVDDGRYRATVIRAGNIEEPQLLTIATKLAIAGVQEARLL
                     SENFELLEEWSDQLPRLREAWERGESLVMKKIPQRKTTLPMSVGSSGYDTQLDYVVKG
                     IIPAVSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGRRVAHGAVLYVVGEGGIGVPR
                     RVKAWEVVHDEQVKNLYLVNRPIFPAAPLDVEEMVIAARQVERETGKPVRMIILDTLA
                     RCFGGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDA
                     EYRIRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDKPR
                     PPVELERIEEAGNKTENHAALWQCIRTRTAHGEACTIALLRDDMKKLGYEMKHFRRWL
                     YKLENDGVIVIDGDDVRPL"
     gene            80866..81111
                     /locus_tag="ACHKIK_000194"
     CDS             80866..81111
                     /locus_tag="ACHKIK_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081434.1"
                     /translation="MNRKTRDKTAPKYKALDMTELSLKVAIRTIDRHAGEGYAKEHPE
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKSAGE"
     gene            81242..81436
                     /locus_tag="ACHKIK_000195"
     CDS             81242..81436
                     /locus_tag="ACHKIK_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081435.1"
                     /translation="MEEIKQSATVMIDHEILNELKKLQFDIVRRMNESGIYGRAATPT
                     IGHLARVLLRERLGIATSEA"
     gene            81440..81601
                     /locus_tag="ACHKIK_000196"
     CDS             81440..81601
                     /locus_tag="ACHKIK_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein NusG"
                     /protein_id="HFO9081436.1"
                     /translation="MAITEREASIIKAIGEETRDTIAPLMEKIAELETRINKLSDEVE
                     GLISLLGEQ"
     gene            81729..82217
                     /locus_tag="ACHKIK_000197"
     CDS             81729..82217
                     /locus_tag="ACHKIK_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO9081437.1"
                     /translation="MALTTKKKRFADALSKGTNPTEAAIHAGYSEKTARVKGWQLSND
                     PEVKKYLKAVTSDVTFQVTKPKKVTKKVTEKVTPERASINTIEMMDNGLPDPIKAMGR
                     ILIDNMDTDPKLALDAAFKLAQFTHRKIGSIGKKEVKKLNAANVSSNRFPIPKPPTGL
                     KH"
     gene            82229..82390
                     /locus_tag="ACHKIK_000198"
     CDS             82229..82390
                     /locus_tag="ACHKIK_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081438.1"
                     /translation="MSEYITDPIDVLTEIMNDKQQPTEIRLQAAAVLMPYFHETLTPC
                     YQEEEDNDF"
     gene            82380..83303
                     /locus_tag="ACHKIK_000199"
     CDS             82380..83303
                     /locus_tag="ACHKIK_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO9081439.1"
                     /translation="MISKNMKGVSAVRAASVLTSCKFSHADAIEMTKKNDPAVSKFLE
                     KSIVAGMTTDNMPSLVAEPIVRELGEYIFSQSVPGKLISSALNLPFGAKLQSISGKGS
                     KWLKEAEEIPVKEGEVNESALKLCKLGAIVMLNNELIKFSTPGTNSAIRDLITSESVK
                     EIDKKFLSSDQEVAGVSPEGVLNGADEAENFIELFKKHIANGNTLATSSLILPVENAL
                     QLTDAEFWQIELLRIPVIVSEQADRMMLIDAQKLIINVEATVITPTDEAVIKTESGTV
                     SLFQNDATAFRAITYCGWQKLDKAVTVLAKS"
BASE COUNT        20846 a        21760 c        20758 g        20226 t
ORIGIN      
        1 cggcactgaa attgccatta cctatgtcta caaaggtgac aaggtgctta agcaatcttc
       61 tgaaaccaaa attcaatttg cttctattgg tgcaaccacc aaagaagacg ctgccaagac
      121 acttgagccg ttaagcgcca aatacaaaaa catcgcgggt gttgaagaaa aattaaccta
      181 tacagatacc tacgcgcagg aaaacgtgac tatcgatatg gaaaaagtgg attttaaagc
      241 cctgcagggt atttcaggaa tcaacgtttc tgctgaagat gccaaaaaag gtatcactat
      301 ggcgcaaatg gaactggtga tgaaagccgc tggttttaaa gaagtgaaat aatcggttgg
      361 cggtcatgct ctaaacatga ccgccaattt tttagccttt tttcacatgc tggcgcgctg
      421 ccagtccacg cagaaaataa cgtaaaaatt gatcgccgca ttcgcggaag tttttatgat
      481 ccggtgcgcg catcatcgct gtaatttccg gcatcgaaac gcggaactgc tgttcggtga
      541 ggatagccag aatgtcatcg gttttcagcg aaaacgcgat gcgtaatttt ttcagcacga
      601 tgttgttatt aatgcgacgt tccggctcca gtgccggagc agactcatcc ttgccgcgtt
      661 tttcataaat caggccattg aggaatgacg acaaaaaaat gtccggacaa cgctgaaaac
      721 cctcttcgtc ttctttacgt agccagacgg cgatctgttc cgcggtggct tcgacattac
      781 ccagcgccag aatacgcacc aggtcattat tattggcttt caaaatgtag cgcacgctgc
      841 gcagaatatc gttacttagc atgaggcctt caggtgttga tgaggcaaaa agccatttta
      901 gcagtctttt acaggccaat cgcctctttt aagcttttca gataacggcg gctgaccggc
      961 acggttaagc cattacgcaa aatcaactcg gcctggccgt tatcttccag acgaatctcc
     1021 tgtaaatgcg cgaggttaac cagatactga cgatggcagc gcagtagtgg tgtacgactt
     1081 tccagggtac gtaatgtcaa ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag
     1141 acaccgctca tccgactgct gacaaatgcc acatctttca tttgcagcaa ataaatccga
     1201 ctatgccccg tacaagggat aaatttcagc gcctgttgat tttccggtaa cagcgaaaca
     1261 tcctgcttgc tgcgctcctg acgcaatcgc gccagcgttt tctccagtcg cgcttcatca
     1321 attggcttca gcagataatc aaaggcatgt tcttcaaagg ctttaatagc gtattcgtca
     1381 aacgcagtga gaaaaacaat atacgggcga tgttccgggt caagcatccc caccatttcc
     1441 agaccactga tgcgcggcat ctggatatcg agaaacagca catccgggcg cagtttatgc
     1501 accgcgccga tcccttccac ggcgtttgaa cactctccaa cgatttcaat atcgctctgc
     1561 tcctgcaaaa atacgcgcag gttctcccgt gctaacggtt catcatcgac aattaagact
     1621 ttaatcatgc ctcgtccctc catggtagtc gtaacgttat tcgggtgtaa ctatcaggct
     1681 cacaggcgac gcttattcca tagtcatcgc caaaccgttc acgtaaacgc ttatccacca
     1741 gattcatccc cagcccactg gcattggtta ccggttgata caaaccggca ttgtcttcga
     1801 tctccagcat caaatgttgc ccctcacgtc gggcgctgat tgccactcgc cctgtatcca
     1861 gcagttgtga tgtcccatgt ttaatggcgt tttccactat cggttgcagg gtaaacgcgg
     1921 gcaattgctg ctgggataat tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga
     1981 agcgcgcctt ttcaatttgc agataagcgt tcacatgttc aatttcgtcg gcgagagtaa
     2041 caaactccga aggccgcttt aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct
     2101 ggctggcctg ttcgctgtcg cggcggatca ccgctttaat ggtgttaagc gcattaaaca
     2161 aaaaatgagg attcacctgg gcgtgaagca gtttgatttc tgactgggtg agcatcgctt
     2221 tttgccgctc atattgcccg gcaagaatct gcgccgaaag cagttgcgca atcccctcgc
     2281 ccagcgtgcg gtttattgaa ctgaataaac ggtttttggc ttcatacaat ttgatggtgc
     2341 ccatcacgcg ctgattttcg ccacgcaacg gaattaccag cgtcgacccc agtttgcatt
     2401 gcggatgcaa agagcaacga taaggtactt cgttgccatc agcgtagacc acttcaccgg
     2461 tttcaatcgc ttttaaggtg taagtcgaag aaatcggttt gccgggtaaa tggtggtcgt
     2521 caccaattcc ggtaaaggcc agcaatttct ctcgatcggt aatcgcgact gcaccaatat
     2581 ccagctcctg atacagcacc tgagccactt tcatgctgtt cacttcgtta aacccctgtc
     2641 gcaaaatgcc ttccgtcgag gctgccactt tcagcgcagt ggcagaaaaa gcagaagtgt
     2701 atttttcaaa catcgcgcgt ttatcgagca atatacgcat aaacagcgcc gcgccgacgg
     2761 tattggtgac catcattggt gcagcaatat tactcaccag acgcaccgca tcttcataag
     2821 gtcgggcgat cgcaaggatg atcagcattt gcaccatttc agcgacgaac gtgacggcac
     2881 cggcggtaat ggggttaaag actttatcag tgcgcccgcg acggatcagg atgctgtgta
     2941 ccaggccgcc gagcaatcct tcaacgatgg ttgagatcat gcaacttagc gcggtcatgc
     3001 cccccatcga atatcgatgt aagccgccgg tcagaccaac cagcccaccg acgaccggac
     3061 cgccgagtaa gccgcccatt accgcgccta tcgcacgggt attggcaata gaatcgtcaa
     3121 tgtgcaaccc aaaccaggtg cccatgatgc agaagatgga aaagacgatg tagcagagaa
     3181 atttatgcgg cagacgaacc gtgacctgca ttaacggtat gaataatggc gttttactca
     3241 ttaaccacgc aatgactaaa aaaacgcaca tctgctgaag cagcagcaac accagattaa
     3301 aatcgtacat acccgcaaac cacacttccc tttaaaacgc gtaacataca ttgcctgcgt
     3361 ttaactttct ttgaactctt gcagaaaaat gagaattcgt gagtacgatc actcaaaatc
     3421 gcctggcaaa aataaaatca ccctatagat gcacaaaaaa cgggcaaaac tacctggttc
     3481 gcaaaactgc gtctaaagtt aaaccgggac ctcgcgagca agggtgagac gatggcgctt
     3541 tacacaattg gtgaagtggc gttgctttgt gatattaacc ctgtcacgtt acgcgcgtgg
     3601 cagaggcgtt acgggttgct gaaaccgcaa cggacagacg gcggtcatcg actgttcaac
     3661 gatgccgata ttgaccgtat ccgcgagatc aaacgctgga tcgacaacgg cgtgcaggtc
     3721 agcaaagtta aaatgctgct cagtaatgaa aatgttgatg tgcagaacgg ctggcgcgat
     3781 cagcaagaaa cattactgac ctacctgcaa agcggcaatc tgcatagcct gcgaacgtgg
     3841 atcaaagagc gcggtcagga ttaccccgcc cagacactca ccacacatct gtttattcct
     3901 ctgcgccgac gacttcaatg ccaacaaccg actctccagg cgctgctggc gatcctcgac
     3961 ggcgtactga tcaactacat cgccatttgt ctggcttcgg cacgtaaaaa acagggtaaa
     4021 gatgcgctgg tggttggctg gaatattcag gataccaccc gtctgtggct ggagggctgg
     4081 attgccagtc aacaaggatg gcgcattgat gtcctcgccc actcgctcaa tcaactacgc
     4141 cctgaactat tcgaaggtcg tacattgctg gtgtggtgcg gtgaaaatcg aacctccgcc
     4201 caacagcagc aactcaccag ttggcaagaa caaggccatg atattttccc actcggcatt
     4261 taatgattcg ttaacaaatg cgctttactg tacaatcctt tcgttaacat aaggagtgca
     4321 ttatgcgcat agctaaaatt ggggtcatcg ccctgttcct gtttatggcg ttcggcggaa
     4381 ttggtggcgt catgctcgca ggttatacct ttattttgcg tgctggctaa gcgcctgcac
     4441 cagcctttca aacaggcggt ctgcgatgat cgccgccagt gccaccagta acgccccctg
     4501 gatcacatac gcggtattaa atccgctaag cccgatgatg atgggcgtac ccagcgtgct
     4561 ggcccctacc gttgaggcga tcgtcgccgt accaatgttg ataatcaccg aagttcgcac
     4621 gcccgccaga atcaccggag ccgccagcgg tagctcgacc ttacgcagtc gctgaccacg
     4681 actcattccc atacctttcg caacttctgt cacgctggca tcgatcgctc ccagcccggc
     4741 aagtgtcgcc tgcaggacgg gcagcacacc gtaaaggatc aaggcgataa tcgctggttg
     4801 cagaccaaag ccgatcaccg gaacggcaat cgccagcact gcgacaggcg gaaaagtctg
     4861 tccaacggcg gcaatagttt ccaccagtgg gcgaaattcc gcgccccacg ggcgagtgac
     4921 agcaattccg gcaccagtgc caatgatcac cgcaaacaaa ctcgaaattc ccaccagcca
     4981 gaaatgagcc agtgccagag ctgcaaaact ttcttgctga taaacgggtc gtggcagttg
     5041 tgggaacaag gcagcaaaca gcggctggct gtaaggcagc cagaaaatca gtgccacaaa
     5101 caaagcaatg agccagaaca gcggatcgcg caacatcttc atacgcttac gcctccacca
     5161 gcagatcctg aaaatgcagc gtaccgcaag gctggccctg cgtgttcacc accggcagca
     5221 cctcgcaacc ccgcgcgaca aacagggaga gcgcatcgcg tagcgtcatc tcttctgcca
     5281 gtgcctcacc ttctgcccgt tcttcgcgac gcacgtaatc cgccacacta cgtaacgaaa
     5341 gcagacgcac acccagttcg ctacgtccaa aaaactggcg gacaaaatca ttcgccggac
     5401 gagtcagcat cgtcagcgga ttgccctgct gcaccacttc accgtgatcc atcaatacca
     5461 gatgttcagc cagccgtagc gcctcatcaa tatcatgagt gaccagcaca atagtgcgcc
     5521 ccagcaaacg gtgaatgcgc gtcatctctt gttgcaacgc gccgcgcgtt accgggtcca
     5581 gtgcgccaaa aggttcatcc atcagtaaga cttgcggatc ggcagccagt gcgcgcgcca
     5641 ctcccacacg ttgctgctga ccaccggaaa gctgatgcgg ataacgctca cgcaaatttg
     5701 actccagccc cagtagcgtc attaattcgt cgatacgatc gtcaatccgt gcccgcgacc
     5761 atttttgtaa ttgcggcacg gtagcgatgt tttgcgccac gctccagtgg gggaataggc
     5821 caatagattg aatggcatag cccatccggc ggcgcaactc cagtactggc agcgagcgaa
     5881 tttcttctcc ggcaaagcgg atctctccgc tgtcatgttc caccaggcgg ttaatcattt
     5941 tcaaggtggt ggatttgccg gagccagatg tgccaatcag caccgaaaaa ctcccttcct
     6001 gaaaattgag attaagatcg ttaacggctt tttgtgcgcc aaacagtttg ctgacatggc
     6061 taaattcaat cattacgttt caccttcagc agtgcgataa gcaaatcgaa cagggcatcg
     6121 atcagcaccg ccagaacaat taccgggatc acccccagca acactaaatc aatggcgctg
     6181 cttagcagcc cctggaaaac cagcgcacca aaaccgcctg cgccgattaa cgccgcaatc
     6241 accgccatac ctacagtttg caccatcacc acccgcaggc tgcgcagaaa taccggtaac
     6301 gccagtggta actgaacatg caggaatcgc tgcgccccgc tcatgcccat cgctctggcg
     6361 ctctccagca catcgcgcgg gatctggttc aaaccgacta ccacgccgcg caccagcggc
     6421 agcaaggcat agagcaccag cgcaatcagt gcgggtgtca ttccggttcc tgctatgccg
     6481 agcttcccca gccacggaaa ggccgtcacc agcgcggcaa gcggcgcaat caacaggcca
     6541 aagagcgcca ccgaaggcac ggtctgaatg acattgagca gagaaaaaat tgccccctgc
     6601 cgagcagtgg aaaagtagca ccagatgccc aacggcacac caatcactaa cgcaggcagc
     6661 accgcaccaa acagcaacgt cagatgttgt gccagcgcgt cgtcaaacac atcctgacgg
     6721 ttggcgtatt ctttcattag tgagagatcg ttaagcgtgc cggagtacag caaccacagc
     6781 ggaataatgg caatctgcat atgcaacaac cagcgccaca gcggatgcgt ggagattcgg
     6841 cggatggcat cgctacaggc cagcaatgcc agcgccgcag ccagccagaa accactgccg
     6901 aggctggtac gcgccagcgc actgccattt tgcgccagtt gtgtcgccgc ctttcccgcg
     6961 ccccacacca gcaatacgaa gacgaattgc gccagaatga gtgcacaaat gctgcctttt
     7021 ttaccgggaa taaaacaggc cgtcagccag gcgcaaccaa cgcccaccag catccagatc
     7081 gtttgcggcc acagttgcca gagatgacgc ccttcacccg agactaaacg attaggcgcg
     7141 tagctgataa acggcagcgc tgctgcgatt gccgtcagca acagcagcag cgccagaaca
     7201 ggattaatac gtagataagt cacgggaaat tacttcgccc accctttttg tttcaggtag
     7261 tcggcagcca cttttttggc atccagtcct tccactgcaa tgctggcatt cagttgctgc
     7321 aatgtttttg catcgaggct ggcgaagact ggctgtagcc actgtgccat ttgcggatac
     7381 tccttcaaca ccgattcacg caccactggt gcaggcgcat agataggttg cacgccttgc
     7441 ggatcgctta aggtttgcag ccccagcgcc gctaccgagc cgtcagtgcc gtaagccatt
     7501 gcagcattaa cgccagaggt ttgctgggca gcggctttga tcgtcaccgc cgtgtcgcca
     7561 ccagccagtg acagcaactg atcctgaccg agcttaaagc cataggcttt ttcaaacgcg
     7621 ggtaacgcat cggcgcgttc gataaactct gccgaggctg ccagtttgaa ggtgccgccc
     7681 tcttgcagat aacgactcag gtcggcaagc gaagtgagtt tatttttctc tgccacatcc
     7741 tgacgcacgg cgatagtcca ggtgttattt gcaggcgcgg gcgtcagcca gattaacttg
     7801 ttgtgctccg aatcgagctt tttgactttc tcgtaacctt gctgcgcgtt tttccacgct
     7861 gcatcgtttt catctttaaa gaagaaagcg ccattgccgg tatattccgg atagatatcc
     7921 agttcaccgg aagtaatcgc cccgcgcacc acaggagtcg ttccgagttg cactttattc
     7981 accgtcggta cgccgtggct ttccagcacc tgcaaaatga tattgccgag tagcgcacct
     8041 tcggtatcga tttttgaacc gactttaacg ggggaagccg cctgtagcgg caggctcacg
     8101 gctgccaaca taaccagtga acctgcccag agctttaaga gtggcatgat gctttcctca
     8161 ttcttttact gttgttttca gcgaattaag agaaaagcat agttgataat ggcggggtta
     8221 gcttgagcgg aaggattcag ttgcagaatc agataaatac ttaagaggca tattcggtcc
     8281 ggcgtttcct gccggatgcg gcgcgagcgc cttatccggc ctacaaaggg cgcaaacgtc
     8341 gcgcccttac taaagcatta cagcaactca aactcgcctt tcttaacgcg tgcggaatca
     8401 gtgccgataa atacattgaa cttgccaggc tcggcgtcat atttcatctg ttgattccag
     8461 aacttcagcg cctcaatatc gatcgggaag ctgacagtct gagtttcgcc cggtttcagg
     8521 gtgattttct caaagccttt cagctgtttc acagggcgac tcatggaagc cgtcacatcc
     8581 tgcaagtaca tctgcactac cgtggccccc tcgcgcttac cggtgttcgt cacctgcacg
     8641 ctggcagtca ctttgccgtc acgcttcatg gtcggcgcag aaagtttcac atcagagacg
     8701 gtgaaagtgg tgtagctcag cccatagccg aacggataca acgccccgtt agcttcatca
     8761 aaataacgcg aagtgtattt gttcggcttg tcggcattat acgggcgacc ggtattcaga
     8821 tggctgtagt acaccgggat ctgcccgaca gaacgcggga aggacattgg cagcttgccg
     8881 gacgggttgt aatcgccaaa caatacatcg gcaattgcat taccgccttc agtccccgca
     8941 aaccaggttt ccagaatcgc atcagcctgc tgatcttctt tcaccagcgc cagcggacgc
     9001 ccgttcatca gcaccagcac cagcggttta ccggtggctt tcagcgccgc aatcaagtca
     9061 cgttggcttt gcggaatagt gatatcggtc cggctggagg cttcgtgcgc catcccctgt
     9121 gcttcaccga ctacagccac caccacatca gattgtttcg ccgtctgcac cgcttcatca
     9181 atcatctctt gcggcgaacg cggatcgact ttgaccgctt cttcatactg attcaggaaa
     9241 tcgataatgc ctttgtcact ggtaacgttc gcccctttgg catacagcac tttaccgttt
     9301 tcaccgacgg aatttttaat cccggtcagt acggtcacgg attgatcggc aacaccggct
     9361 gcggaccagc tgcccatcac gtcacgttta ctgtccgcca gtggcccaac caccgcaatg
     9421 gtggccgatt ttttcagcgg taacgtttcg agacggtttt tcagcaacac caggctttcg
     9481 cgcgccactt cacgcgcttc tttacggtgc aggcggcttt cggcattggt atccaccggg
     9541 tcagactctt tcggccccaa atggctgtat gggtcgttaa acaatcccat atcatattta
     9601 acgttcagta catggcgggc agcatcgtcc agctcttcca tcgtcacttt gccggatttg
     9661 atcaacccag gcagatactt cgagtaatac tcgtcgctca tactcatgtt gattccggat
     9721 ttcagcgcca cgcgcaccgc atcttccggg tctgccgccg tgccatgttt aatcagctct
     9781 ttgattgcac cgtgatcgga aacggtgatg cctttaaagc cccactggtc gcgcagaaca
     9841 tctttcagca gccaggaatc ggaggtggct ggcgtgccgt tcagcgagtt cagcgccacc
     9901 atcaccgcgc cgctgcctgc gtccagcccc gctttgtacg gcggcatata atcattaaac
     9961 aggcgctgcg gactcatatc gacggtgttg tactctttac cgccttctac cgcgccgtat
    10021 gcggcaaagt gtttgacgct ggtcatcacc gagtagcgat ctgccgggct tttaccctgc
    10081 atcgcttcca ccatggtttt acccattgtt gaggtgagat acgtatcttc gccaaaacct
    10141 tcggaagcac gtccccagcg cggatcgcgc gagacatcga ccatcggtgc ccaggtcata
    10201 ttcaggccat catctgccgc ttcataagca gagacacgtc cgaccgtttt cactgcatcg
    10261 aggttaaaag acgaggccag accgaggcta atcgggaaca ccgtgcgctg accgtgcagc
    10321 acgtcgtaag caaagaaaag aggaattttc aggcggctta attccatcac ctgatcctgc
    10381 atggcgcgga tatcctgacg ggttacggtg ttgaaaatcg ccccaacctg accgtctttg
    10441 atcatctcgc ggatcgcctc tttcgggtta tccgggccga cgctgattaa gcgcagctga
    10501 ccaattttct catcaactgt cattttctta agcagttcgg tgacgaacgc atcccgcgct
    10561 tcgggcgtta atggatggtt gccgaataaa tcatccgcca gtgctggctg cagggccaga
    10621 ctcaccgcga ttcctactga acatagccat ttcatatgga tttttcttcc tctgttgccc
    10681 gacgaggcag cgaaaatggt gcaaaaaccg tagtttgcca taagcatgat ggagagagaa
    10741 aaagaatgct cagtttattg tctgaatttt caaaatattc actcgctgaa ttgtcataca
    10801 aggcgctatt ctagtttgtg atattttttc gccaccacaa ggagtggaaa atgtcttcca
    10861 tgacaacaac tgataataaa gcctttttga atgaacttgc ccgtctggtc ggtcattcac
    10921 acctgctcac cgatcccgca aaaacggccc gctatcgcaa gggcttccgt tctggtcagg
    10981 gcgacgcgct ggctgtcgtt ttccctggct cactactgga attgtggcgg gtgctgaaag
    11041 cctgtgtcac cgccgacaaa attattctga tgcaggctgc caatacaggc ctgaccgaag
    11101 gatcgacgcc aaacggtaac gactatgatc gcgatatcgt gatcatcagc accctgcgtc
    11161 tcgacaagct gcacgttctt ggcaagggcg aacaggtgct ggcctatccg ggcaccacgc
    11221 tctattcgct ggaaaaagcc ctcaaaccgc tgggacgcga accgcactcg gtgattggat
    11281 cgtcgtgtat aggcgcatcg gttatcggcg gtatttgtaa caactccggc ggctcgctgg
    11341 tgcaacgtgg cccggcgtat accgaaatgt cgttattcgc gcgtataaat gaagacggca
    11401 aactgacgct ggtgaaccat ctggggattg atctgggcga aacgccggag cagatcctta
    11461 gcaagctgga tgatgatcgc atcaaagatg acgatgtgcg tcacgatggt cgtcacgccc
    11521 acgattatga ctatgtccac cgcgttcgtg atattgaagc cgacacgccc gcacgttata
    11581 acgccgatcc ggatcggtta tttgaatctt ctggttgcgc cgggaagctg gccgtctttg
    11641 cggtacgtct agataccttc gaagcggaaa aaaatcagca ggtgttttat atcggcacca
    11701 accagccgga agtgctgacc gaaatccgcc gtcatattct ggctaacttc gaaaatctgc
    11761 cggttgccgg ggaatatatg caccgggata tctacgatat tgcggaaaaa tacggcaaag
    11821 acaccttcct gatgattgat aagttaggca ccgacaagat gccgttcttc tttaatctca
    11881 agggacgcac cgatgcgatg ctggagaaag tgaaattctt ccgtccgcat tttactgacc
    11941 gtgcgatgca aaaattcggt cacctgttcc ccagccattt accgccgcgc atgaaaaact
    12001 ggcgcgataa atacgagcat catctgctgt taaaaatggc gggcgatggc gtgggcgaag
    12061 ccaaatcgtg gttggtggat tatttcaaac aggccgaagg cgatttcttt gtctgtacgc
    12121 cggaggaagg cagcaaagcg tttttacacc gtttcgccgc tgcgggcgca gcaattcgtt
    12181 atcaggcggt gcattccgat gaagtcgaag acattctggc gttggatatc gctctgcggc
    12241 gtaacgacac cgagtggtat gagcatttac cgccggagat cgacagccag ctggtgcaca
    12301 agctctatta cggccatttt atgtgctatg tcttccatca ggattacata gtgaaaaaag
    12361 gcgtggatgt gcatgcgtta aaagaacaga tgctggaact gctacagcag cgcggcgcgc
    12421 aataccctgc cgagcataac gtcggtcatt tgtataaagc accggagacg ttgcagaagt
    12481 tctatcgcga gaacgatccg accaacagca tgaatccggg gatcggtaaa accagtaaac
    12541 ggaaaaactg gcaggaagtg gagtaaaaat tacggatggc agagtatcgc catccgaatt
    12601 cacttaatcg ttctgtgccg tctgccccgc cgccgccatt tgggcggctt tttgtttttt
    12661 atagctcaac gctgctgccg gcacaggcat cactttaccg gtttcaatcc aggtacgcag
    12721 gcggctggca tcggcaaaat gggtatattt gccaaacgcg tccatcacta ccagcgccac
    12781 cggtttatta ttgataaccg tacgcatcac cagacaatgg cccgccgcat tggtaaagcc
    12841 ggttttggtt aactgaatat tccagttatc gcgatacacc agatgattag tattgcggaa
    12901 cggcagcgta tacgtcgggt tagagaaggt tgccatatct tcccgggtag tacttaactg
    12961 cccgatcaac ggatactgtt tgctggcaat gagcagtttg gttaagtcac gggcagttga
    13021 aacgttatgc accgacaatc cggtaggttc aacaaagcgc gtgttgttca ttccgagcga
    13081 tttcgctttc gcattcattg ccttaataaa ggctttgtaa ccaccgggat aatggtgcgc
    13141 aaggcttgcc gccgcgcggt tttctgaaga catcagcgcc agcaacagca tatctttacg
    13201 gctgatttcg ctattcagtc gtacgcgcga atagacccct ttcatctccg gcgtctggct
    13261 gatatccact tttagttttt catccagcgg cagtcgtgca tccagcacaa ccatcgcggt
    13321 cattaatttg ctgatagacg caatcggacg caccagatcc gggtggttcg aatagatcac
    13381 tttgttggta ttcagatcaa caatcatcgc gctaccggag gcaatttccg gttgtgaagc
    13441 ggtggtagcg gctgccgttt tcgcaacggc ctgcggtgcc aaaggcacag ccagcatcag
    13501 ggccaggcta aataaagaaa ctcgaaattt cggcatgatg agcattcaga tagtggttca
    13561 cgcgcacggg ttgcgcaccg ccggagtaag gatttactga ggctagcgac gccatcataa
    13621 cgagcaaaaa gtgcgatcgt caaaggagaa tcgtgaggaa atgctgcatt gctgacattt
    13681 acgccagcaa tgcaatgtca aagaactttc tagaacaggc gatacccgta gccccatagt
    13741 ataacggtta gggcgagcag cacttccagt accagcacgc caatcgccag cgtcgaactg
    13801 gagaagctaa ggccttcctc tttgttgata ttcaggaagc tcggaatacc aaggtaaagc
    13861 aggtagccgg tgtaaaacag cgccaccgtg ccgaccagcg cacacaacca gaccagcgga
    13921 taaagcgcca ccagaccgct taaaaacagc ggagttgcaa cgtagcccgc gaagaccatg
    13981 cagtgcgcaa gtgacggacg ctgcgggtaa ttgcgcgcca tccaccagat gacccgcccc
    14041 atcaccgcaa ccccagcgag cataacgcca taaaacagga cagccagcgc cagtccggta
    14101 aaccaggaca acttcaggat agtgccatcg ccaaaattcc agccaatctg tgtagtgcca
    14161 atgaaggcgc aaatcaccgg aatcgccgcc atcagcaaaa cgtggtgggt gtaatggtga
    14221 gaaatcgttt cgttttcgcg attaatcacc tgcatttcac gatcgggatg ggaaaacagt
    14281 ccccagacat ggctcatacc gcccccttgt tgtgagttca tgaacctgac agttcaagta
    14341 taagtcagct tgtgattatt ttttgttcgc tcatgtaatt tgcaccgtct gatgtacgtc
    14401 ctttttcagc gtgaatgatt tccagggtgt atgcttacag gcaaccaaag ggagacagac
    14461 tggcctatgg atctcaatac actcatctca caatatggtt atgccgcgct ggtgatcggt
    14521 agcctggcgg aaggtgaaac cgtgactttg ctgggaggcg ttgcggcgca tcaggggcta
    14581 ttaaagttcc cgctggtggt actttctgtg gcgcttggcg gcatgattgg cgaccaggtg
    14641 ctttatctgt gcgggcggcg gtttggcggc aagctgttac gccgtttctc gaaacatcag
    14701 gataaaattg agcgggcgca gaaacttatc caacgccatc cgtatctgtt tgtcattggt
    14761 acgcgcttta tgtatggctt tcgggtgatt ggcccgacgc tgattggtgc cagccagctg
    14821 ccgccgaaaa tctttctgcc gcttaatatt ctcggcgcat ttgcctgggc gttgattttt
    14881 accactatcg gttacgctgg tggtcaggtg attgcgccgt ggttgcacaa tctcgatcag
    14941 catttgaagc actgggtctg gttgattctg gtcgtggttc tggtggtagg cgtgcgctgg
    15001 tggctgaaac gacgcgggaa gaaaaagccg gataatcagg cgtaaaacca ttgccctgga
    15061 taaggcgttc gcgccgcatc cgacatccgg tgcaccatgc ctgatgcggc gcttgtgcgt
    15121 cttatcatgc ctaccaaaat tttacccatt gaaatatcca cacacctcac tgcgctattc
    15181 tgggttgaac tgcggattcg ccaacataaa gccgccatcc actatcaacg actgcccggt
    15241 ggtgtaattt gcgccctccg aacaaagcca cgccaccagg ctggcaatct catacgttgc
    15301 gccaaaacgc cgcagaggaa tcgaaggctc cgcgtcgggc ttcacgtcgc tatcatccat
    15361 gccgttcatt ggcgtagcga tcgccccagg tgcgactgcg ttcaccaaaa tcttatgcct
    15421 gaccaactcc agcgccattg ctttggttaa cccgccgagc gcatgtttag cggctgtgta
    15481 ggcgctggca tccggcagcg gcgtatgttc atgtaccgac gtaatgttga tgatgcgacc
    15541 accctgccct tgtttcacca tctggcgggc cgcaatttgt gagcataaaa atgcaccatc
    15601 gacatcaacg gtaaaaatct tgcgccactc atcgaaagcc atatcaagaa acggcgcttt
    15661 ggtcattgcg cccgcattat tcaccagcac atcaatacgc cccagccgtt gaatgagttt
    15721 ctccagcgcc tgtgcacctt ctggcagttt accgagatcc agctgcacaa tctccgcacg
    15781 tacgccgtgg ctaactacct cacgcgcggt atcttttgcc ccttcttcat ctgagtgcca
    15841 ggtaatgcca atatcaaacc cctgctgcgc cagtaataac gcgcactctt tgccgatccc
    15901 cgaatcggag gcggtaataa tcgcaacctg tgccatcgag ttctccactt aacgctgaat
    15961 aaacgttaag tatagaaggc gcatatcatc agcgtttgta acccccgccc aacgcaccag
    16021 tgagttgaat ggaagcatcc agccactgcc cttgcaataa caggccattg gcccgctcac
    16081 gcagcgcggg gattctggct tcgctgacgc gggaaccagc aatgatgccc gcgttaaagc
    16141 gcgcctgcgc aagacctacc acacgcaagg catcgcgctc aatttgcgcc tgatgctggt
    16201 ttttctccgc aagtgtctga acctgactgg ctgcccgcgc cacgtcattc accgcttcaa
    16261 ccaccgcttt gttgtagctg gcgatagaca agttgctttc ggcttttgcg atatcgagat
    16321 tggcgttaag acgaccacta tcgaaaatgg gtagcgtcag gcctgccgta acgcccattt
    16381 gctgcgcgga atgacggaac agatcgctta agtgcaacgc atcctgttgc aggaaggcca
    16441 tcaggttgat gtcaggataa aatgccgctt ttgccgcatc aatggtgctt agcgatgact
    16501 caacgtacca gtgcgccgcc tgcacatctg cccgccgggc cagtaaggag taccccagtt
    16561 catcaggaag ctggcttgcc actttcggca acgcgaccgg tttaagcttc aatgactttg
    16621 tctggttatt tgtaagtgcg cttaaccgtg cctcaataat tttcattttc cccgcgacat
    16681 cgttgagctg ctgccgggtt ttgctggcat taatatcggt ttccacacct tcaactgaag
    16741 aagtaatccc gttctgatat agctggcgat cggtcgcgat aatggtgttc tgctcttttt
    16801 ctatttgctg caagaccgtg tttaacgccg cctgggtttg ccactcccag tacaggcggg
    16861 ctacgctgcc agccagcaat tggcgggttt gctcgcgttc cgccgcccgt gctttaaccg
    16921 tacccaggcg ggcagtaacc tccgcccgat tctttcccca gatatcgaga tgccagcccg
    16981 ccgttaagcc aaaagttccg ttggtgtacc acggaccggt cgtacctgcg gccggatcgt
    17041 tcagagcaaa cggccccatt aagccttctg ccgacatttt ttgccgctcc atatccgcag
    17101 aaaagtcgat ctgcggacca tcctgagtag caattgcctt cgcctgggct tcagctagct
    17161 gaatgcgctg ttcagccacc tgcatatccg gtgcgttctg tagtgcattg ttaattaagg
    17221 aagtgagttg attatcgtga tactccagcc accattggct gtctggccaa ccatttttca
    17281 gcgccgtggg taatgcggtg tcaacttgtg cagcgggcgt ttgctggctt aacgcctggc
    17341 gggtttcatg cataggcgca cacccggcca gcatcagtaa cagcggaaaa caggcgatgg
    17401 cgggataaaa ggaatcacga ttcatggggg aataatcagg taagaaaagg tgcgcggaga
    17461 ttaccgtgtg ttgcgatata ttttttagtt tcgcgtggca atacatcagt agcaataaaa
    17521 cgacatatcc agaaaaatat acactaagtg aatgatatct tccgatttat cttaatcgtt
    17581 tatggataac ggcaaagggc ttcgtttttt cctatactta ttcagcactc acaaataaag
    17641 gaacgccaat gaaaattata ctctgggctg tattgattat tttcctgatt gggctactgg
    17701 tggtgactgg cgtatttaag atgatatttt aaaaatcaga tatggcatat aagttgtcag
    17761 gtcaggaact agatattttg tgtacttgat ttgcaaacag ctccggtgtt ggtggcgtca
    17821 aaatcgctga agcgttcccc gaaggccggg gcagcccaca tggatgtggg ctgagggcgc
    17881 gtcttacaag gacgttacct cgcgcccgac ccggtagacg aaggggataa gctgaaggta
    17941 ccgcgcagcg gcgattttgt tcgccagagc ccgggggtgc agggggagga ggcgattgtc
    18001 ctccccctgc gcgctccttg cgccagtggc aatatgtggc ttagctcatg aaaggagcgc
    18061 aacaagatga tgaagctgaa taagcgattc attgtctctg ataaactgcg attccactat
    18121 tatcgcagct tatcgatatc aattgcctga atagcccttg caatatcagg ggaattattc
    18181 aacacccgaa catgctgaaa taattccgtt gcttcatcgt attctttacg caaataactc
    18241 aaccactgtt taatccgcgc aacgtgatat aacccggtat cgccctgctt ttccagacgg
    18301 gtatattttt gcagcaaagc aaccacctcc ggccacggca ttcgcggttc gttatatttt
    18361 actacccggc tcaggttggg aatattgagc gccccgcgac caatcatcac tgcgtcgcag
    18421 ccgctgatcg caatgcattg ttgcgcgctc tgccagtccc agatttcgcc gttggcaatc
    18481 accggaatat tcagccgctg gcgaatctcg ccaatcgcct gccagtcaat atgctccgcg
    18541 cggtaaccct gctcttttgt ccgtccatgc accaccagct ccgtagcacc agcctgttga
    18601 accgcatcgg cgatttcaaa tttcttctca ccgctgtccc agcccagacg cactttcacg
    18661 ctgacgggca aatgcgccgg tacagcttca cgcatcgctt ttgcaccctg gtagatgagt
    18721 tcaggatctt tgagtaacgt cgccccacca ccgctaccgt tgaccgtttt cgacgggcag
    18781 ccgcaattga gatccacgcc ccaggaacct aattccaccg cacgggcggc gttctctgcc
    18841 agccattgtg ggaactgacc caacaactgc acgcgcacca gcgtaccgga tggtgtccgg
    18901 ctggcgtttt gtagctcagg gcaaatgcga tgaaaaactt ttaccggcag cagttgatcc
    18961 accacgcgga caaactcggt gatgcacaga tcgtagtcgt taacttcggt cagcaattca
    19021 cgcaccagag agtcaagcac accctccatc ggagccagta acacacgcat atcttcaccc
    19081 gcaaaaaaat gaggcgctat gttagcgcct atggtcaacg gattaaaggt ctgcaaagag
    19141 ggattgctgc aaactgtaat ccctctctat gcttgatgta tgcctgagtt cgttgcgcat
    19201 tgcgtaatgc cggggagcga aaaaataagc gctgatttct taatgtgatc ggtagcacgt
    19261 tttacgaatt aattgtatga tgaatccatc tcatctgggg tgttgattat gagtaagaca
    19321 ctgaacatta tctggcaata tttacgcgct ttcgtcctga tttatgcctg cctgtatgca
    19381 ggcattttca ttgcttccct gctaccggta accattccgg gcagcatcat cggtatgctg
    19441 atcctgtttg tcctgctggc cttgcaaatt cttccggcaa aatgggtcaa tccggggtgc
    19501 tacgtactga ttcgctatat ggcgctattg tttgtgccga ttggcgtagg cgtcatgcaa
    19561 tattttgatt tgctccgcgc acagtttggc ccggtagtgg tttcctgtgc aatcagtacg
    19621 ctggtggttt ttctggtggt gagctggagt tcgcaactgg tacacggtga acgtaaagtc
    19681 gtaggtcaga aaggatcaga agaatgatgg caaatatctg gtggtcatta ccgctgacgt
    19741 tgattgtctt ttttgccgcc cgcaaactgg cggcacggta taagtttccc ctgcttaacc
    19801 cgttgctggt agcaatggtg gtgatcattc cttttttaat gctgaccggc atctcttacg
    19861 acagctactt taaaggtagc gaagtgctaa acgatctgct gcaaccggcg gtcgtcgcgc
    19921 tggcctatcc tttatatgag cagctacacc agatccgcgc gcgctggaaa tcgatcatca
    19981 ccatctgttt tatcggcagc gtggttgcga tggtgacggg gacttccgtg gcattgttga
    20041 tgggcgcttc accggaaatc gccgcgtcaa tcctgccgaa atcagtcacc acgcctattg
    20101 caatggcggt tggcggcagt attggcggta ttccggcaat cagcgcagtt tgcgtgattt
    20161 tcgtcggcat cctcggcgcg gtatttggcc atacattgct taatgcgatg cgtattcgta
    20221 ccaaagctgc gcgcggtctg gcaatgggga ctgcctcgca cgccctcggt acggcgcgct
    20281 gcgccgagct ggattatcag gaaggtgcat ttagttcgct agcgctggtg ttatgcggga
    20341 taattacttc gctgatcgca ccgttccttt tcccgattat tctggcagta atgggctaaa
    20401 atttgcgatg cgtcgcgcat ttttgatgta agtttcacgc gttgcataat taatgagatt
    20461 cagatcacat ataaagccat aacgggttcg taaactgtta tcccattaca tgattatgag
    20521 gcaacgccat gcatccacgt tttcaaaccg cttttgccca acttgcggat aacttgcaat
    20581 ctgcactgga acctattctg gcagacaagt acttccccgc tttgttgacc ggggagcaag
    20641 tctcatcgct gaagagcgca acggggctgg acgaagacgc gctggcattc gcactacttc
    20701 cgctggcggc ggcctgtgcg cgtacgccat tgtcgaattt taatgttggc gcaattgcgc
    20761 gcggtgtgag cggaacctgg tatttcggtg ccaatatgga atttattggt gcgacaatgc
    20821 agcaaaccgt tcatgccgaa caaagcgcga tcagccacgc ctggttgagt ggtgaaaaag
    20881 cgcttgcagc catcaccgtt aactacacgc cttgtggtca ctgccgtcag tttatgaatg
    20941 aactgaacag cggtctggat ctgcgtattc atctgccggg ccgcgaggca cacgcgctgc
    21001 gtgactatct gccggatgcc tttgggccga aagatctgga gattaaaacg ctgctgatgg
    21061 acgagcagga tcacggctat gcgctgactg gtgatgcgct ttctcaggcg gcgattgcag
    21121 cggcaaaccg ttcgcacatg ccttacagta agtcgccaag cggtgtcgcg ctggaatgta
    21181 aagacggtcg tattttcagt ggcagctacg ctgaaaacgc cgcattcaac ccgactctgc
    21241 caccgttgca gggagcgtta attctgttga atctcaaggg ttatgattac ccggatatcc
    21301 agcgcgcggt tctggcagaa aaagccgatg cgccgttgat tcagtgggat gccacctccg
    21361 caacgctgaa agctctcggc tgtcacagta tcgaccgagt gcttctcgct taagcctggt
    21421 gccggatgcg gcgtgaacgc cttgtccggc ttgccagccc tctcctggtg tcgaaattcc
    21481 cggcaaacag tttgccgttt cttgcgcaaa accagcgggt aaagtagcct gatggaaatt
    21541 ttccttagat cgagtctcct gcatgttaaa gcgcgtgttc ctcagcctgt tagtcctgat
    21601 cggcttgctg ctgttgactg tgctcggcct cgatcgctgg atgagctgga aaaccgcgcc
    21661 ttatatctac gacgaattgc aggatctccc ctaccgccag gtcggtgtgg tgctcggaac
    21721 agcaaaatat tatcgtactg gcgtaattaa tcagtattat cgctaccgca ttcaaggagc
    21781 gattaatgcc tataacagcg gtaaggtaaa ttatctatta ctgagcggcg ataacgcatt
    21841 gcaaagttat aatgagccga tgaccatgcg caaagattta atcgctgctg gtgtcgaccc
    21901 atcagatatt gttctcgatt acgcaggctt tcgtacgctg gattccatcg tgcgtacacg
    21961 caaagttttc gatactaatg atttcattat tatcacccaa cgtttccact gtgagcgggc
    22021 attatttatt gcgctgcata tggggattca ggctcagtgt tatgccgtac cgtcaccgaa
    22081 agatatgctg tcagtacgta ttcgcgaatt tgccgcccgt ttcggtgcgc tggctgacct
    22141 ttatattttt aaacgtgaac cgcgtttttt agggccgctg gtccctattc cggctatgca
    22201 ccaggtgccg gaagatgcgc aggggtatcc cgccgtcaca cccgaacagt tacttgaatt
    22261 acaaaagaaa caaggaaagt agttatggat gtacagcagt tttttgtcgt tgccgttttt
    22321 ttccttattc cgatattttg tttccgcgaa gcatggaaag gctggcgcgc aggcgcgatt
    22381 gataaacggg ttaaaaatgc accggaaccg gtgtatgtct ggcgagcaaa aaatcccgga
    22441 ctctttttcg cttatatggt ggcatatatc ggcttcggaa ttttatctat cggcatgatt
    22501 gtttatctta ttttctatcg ttaatatttt ctcttttctg ttaattattg agaataatta
    22561 ttacttcact caataagctg cggatataac attctgaacc gcagctattt gtgaatcctt
    22621 tcacagtata aatttcccca cgcgcatagc cttaatatca gcacgcaata tttatactct
    22681 aaataattcg agttgcagga aggcgacaag tgagtgagtc cccaggagct tacataagta
    22741 agtgactggg gtgagcgaac gcagacgcag cacatgcaac ttgaagtatg acgagtatat
    22801 ttagcgttcg ccttaattac tcatagcatt aaggaagatc acatgccgca acaaaattat
    22861 ctggatgaac tcactccggc ttttacgcct ttactggcga ttaaagaagc ctctcgctgt
    22921 ttattatgtc atgacgctcc ctgtagtcag gcttgcccgg cacagaccga tccggggaaa
    22981 tttattcgct caatctactt tcgtaatttt aaaggcgctg ccgagacaat tcgcgaaaat
    23041 aatgccctcg gtgccgtttg tgccagagtg tgcccgacgg agaaattatg tcaaagcggt
    23101 tgtacccgtg ccggtgttga tactcccatt gatatcggtc gcttacagcg ttttgttact
    23161 gattttgaac aacaaaccgg gatggaaatt tatcagcccg gtactaaaac gctcggcaaa
    23221 gtcgcgatta ttggcgcagg tcctgccgga ttgcaggcca gtgtaacact gacaaaccag
    23281 ggttatgacg tcacgattta tgagaaagaa gcgcaccccg gtggctggtt gcgtaacggt
    23341 attccgcaat tccggttacc gcaatcagtg ctggatgcag agatcgcccg tattgaaaaa
    23401 atgggcgtga ccattaagtg caacaacgaa gtcggtaaca cactcaccct tgagcagctg
    23461 aaagcagaaa accgcgcggt actggtcacc gtggggttat cgagcggttc cgggttatcg
    23521 ctgtttgagc atagcgacgt tgagattgcc gtcgacttcc tgcaacgtgc acgacaggcg
    23581 caaggcgata tcagcattcc acaaagcgca ttaattatcg gcggcggtga tgttgcgatg
    23641 gacgtagcca gcacgctgaa agttctcggc tgtcaggcgg taacctgcgt agcgcgtgaa
    23701 gagttagatg agtttccggc aagcgaaaaa gagtttgcca gcgcccggga gctgggcgtt
    23761 tcgatcatcg atggattcac gccagtagcc gtggaaggca ataaagtcac gtttaagcat
    23821 gtacggttac cgggcgaact gacgatggcg gcagataaaa ttattctcgc cgtcggtcag
    23881 catgccagac tggatgcctt tgcggagtta gagccgcagc gtaacaccat caaaacacaa
    23941 aattatcaga cccgcgatcc gcaagtcttt gctgctggcg atattgttga gggtgacaaa
    24001 accgtggtct atgccgtgaa aaccgggaaa gaagccgccg aggcgattca tcactattta
    24061 gagggagctt gctcatgtta acgaaagatc tttcgattac tttttgcggc gtgaagtttc
    24121 ccaacccgtt ctgcctctct tcttcgcctg taggcaactg ctatgagatg tgtgccaaag
    24181 cctacgacac aggttggggc ggcgtggtgt ttaaaacgat cggctttttt atcgccaacg
    24241 aagtctcgcc gcgttttgat catctggtga aagaagatac cggttttatc ggcttcaaaa
    24301 atatggagca gattgctgaa catccgttgg aagagaatct ggccgccctg cgtcagctga
    24361 aggaagatta cccggacaaa gtattgatcg cttcgatcat gggggaaaat gagcagcaat
    24421 gggaggagct ggcgcgcctg gtgcaagaag ctggcgcgga tatgatcgag tgtaacttct
    24481 cctgtccgca aatgacttct catgcgatgg gtagcgatgt cgggcaaagc ccggagctgg
    24541 tagaaaaata ttgtcgggca gtgaaacggg gttccacgct gccaatgctg gcgaagatga
    24601 cgccgaatat cggtgatatg tgcgaagtgg cgctggcggc gaagcgcggc ggcgcagatg
    24661 gcattgcggc gattaacacc gttaaatcca tcaccaatat cgatcttaat cagaaaatcg
    24721 gtatgccgat cgttaacgga aaatcgagta tttccggata ttccggtaaa gcggtaaaac
    24781 cgatcgccct gcgcttcatt cagcagatgc gcacccatcc agaactgcgc gatttcccaa
    24841 tcagcggtat cggcggcatt gaaacctggg aggatgcggc tgagttttta ttgctcggcg
    24901 cagcaacgtt acaggtgacc accggcatca tgcagtacgg gtatcggata gtggaagata
    24961 tggcaagcgg gttgtcgcat tatctcgccg atcagggatt tgattcgctg caggagatgg
    25021 taggtctggc gaataacaat attgtcccgg cggaagattt agaccgcagt tatattgtct
    25081 atccccgtat caatcttgat aaatgtgttg gctgtggacg ctgttatatt tcctgttacg
    25141 acggcggtca ccaggcgatg gaatggagcg agaaaacccg cacaccgcat tgtaataccg
    25201 agaaatgtgt gggttgtctg ctttgtggtc acgtctgccc ggtgggttgt attgagctcg
    25261 gggaagtgaa gtttaagaaa ggcgagaaag aacacccggt aacgttgtaa aaccagagac
    25321 gcatccggca tttggcaccg attgccggat gcagcgcatc cggcaatatc cacaatcaca
    25381 tcacttctta cgcgcgtatt tcagtgaatc cagcgctacg gcgaagataa taatcgcccc
    25441 tttgatgatg tactgccagt atgggtttac gccgatatac gtcaggccat agttgatgac
    25501 ggtaaaaata attaccccgg tcaccacgcc aatcaccgtc cccacaccgc cgctgaacga
    25561 tacaccgcct accacgcacg ccgcgatagc atccagctca tacataaagc cgaggttgtt
    25621 ggtggcagag ccgatacgtc cggcttctaa catcccgcca aaggcataga acacgccaga
    25681 caacgcgtag atcatcagca ggttcaggcc gacgttgaca ccagatactt ttgccgcttc
    25741 cgggttaccg ccaatggcaa aaatgttctt accgaagcgg gttttgttcc acaacaccca
    25801 gacgaacgcc accgcaatca acgcgtagaa ggtgatgtaa gagagacgga aactccccag
    25861 cgcgacaaag ccctgagcaa aggtagagaa gccactgtca aaaccagaaa ttggcgacgc
    25921 cccgacaaag tcgtaataga gcgagttgat tccatagacg atgatcatcg tgccgagcgt
    25981 ggtaatgaac ggcgtcacgt tgagataagc gataatcaga ccgttgatca aaccgatcac
    26041 cgcaccaatg gcacagacaa tcagaataac cagcgcaatc ggcatcgtcg ccatttccgg
    26101 gaacactttg ttggcgttat ccatggactg caataatgtc gcagccacca ctgccgccag
    26161 ccctacctga cgaccagcag aaagatcggt cccctgggtg acaattaacc ctgccacacc
    26221 gagcgcgata ataatacgca ccgatgactg ggtgagaata ttacttaagt tcaacagact
    26281 taaaaatgtt gggtcctgga aaataataat cgccagcaaa actaaaagaa cgacgtaaat
    26341 accgccctct ttcaggtaag taagaaaact tttcttattt aacgcactca tgggaagccc
    26401 ctaatcttaa aggtgcaaag acgcaagacg cagaatttcg ttttgcgttg ttgtttttgt
    26461 atcgacaatt ccggaaacga gaccattgct catgaccaga atacggtctg ttatccctaa
    26521 caactcaggc atttcagagg agataataat aatccccttg cctttcttcg ccagttcggc
    26581 aattaactga taaatttcaa acttcgcccc gacatcaata ccgcgcgtcg gttcatcgag
    26641 cattaatatt tctggttgcg ttagtagcca gcgaccaata atcacctttt gctgattacc
    26701 accggagagc gaaccaattt gcgtccgatg acccggcgtt tttacccgca tcgaatcaat
    26761 cacccactgg gtatcgcttt tcatccgcga gttatccagt aaaccaactt tatttttgta
    26821 gttgcgaata ttggaaatta acgagttaaa actaatatcc agataggcat aaattcccgt
    26881 tgagcggcgc tcctcagtta ccagtgcaaa tccgtggttt atggcttcgt tggcattatg
    26941 gttattgatc tgtttgccgt gcaacgtaat ggtgccagcc gatttctcgc gaataccaaa
    27001 taacgtctca acaatatcgg tacgtttcgc ccccaccagc ccggcaatac cgaggatctc
    27061 ccctttatgc agatcaaacg agacatcgcg aatcgacggc tggcgcagtg acgttaggtt
    27121 acgcacctcg aggatgactt cgcccggctt gttttctttg tcaggaaaac gctgattaag
    27181 agaacgccca accatcatgg cgatgatctt gtccatcgtc agtcctgcca gcggttcggt
    27241 ggcgatccac tgaccgtcgc gcaatacggt aacttcatca cataactgga agatttcttc
    27301 cattttatgc gagatataaa caataccgca gccgcgctct tttaatttac gaataatagt
    27361 gaacagatga ttgacctctt tttcggttaa cgaagaagtc ggttcatcca taatcacaat
    27421 tttcgcgtta taggaaaacg ctttggcgat ttcgatcatc tgcatttgcg aaacggataa
    27481 tgtgccgacg cgcgcacgcg gatcgatatc aatatccagt tcatcaaaaa tcgctttggt
    27541 ttcgcggtac attttgtcct gatcgacaaa catgcctttg gtgggatatc gccccagcca
    27601 catgttatcc atcaccgaac gttgtaatac caggtttaac tcctggtgta ccatcgaaat
    27661 accattttcc agtgcctctt tggcggaatg gaaatcgatc tctttaccct ggaataaaat
    27721 ggtgccggag tctttttgat aaataccaaa caggcatttt aataatgtcg attttcctgc
    27781 accgttttcc cccattaatg catggataga atgtggccgg acttttaaat taacgttatc
    27841 aagtgcctta acaccaggaa aggacttgtt gataccgctc atttccaaca agtattcccc
    27901 ggaagacgga gtcgttgagc tgaccatata attttacctt gttggccata caataagggc
    27961 gcagtaaacg actgcgccca atcagtctta tttcttgctg aattcagcca ggttgtcttt
    28021 atctacgcca acataaggta cgcggaccac tttgttgtcg attttccagt tggtgccatc
    28081 agccgcacct ttgccatcgg ccaggttttt cgccagatca aaggtcgctt tcgcctggtt
    28141 gttagcatcg ttcagtactg tgcccgccag tgcaccggat ttcaccagcg ccagcgcttc
    28201 tggcagcgca tcgacgccaa acaccggaat gctggacttg ttgtgtgctt tcagtgcttc
    28261 taccgcgccc attgccatcg catcgttgtt ggcgataacc acttcgattt tgttggcgtt
    28321 cgggccagac agccaggcgt ccatcttatc tttcgcctga gcggtatccc acatagcggt
    28381 atctaactgt aactgttcag ttttaatgcc tttgtcgttc agctctttaa tcacgtaagt
    28441 ggtacgtgct tctgcatccg gatggcccgg ttcacctttc agcaatacga attgaatctg
    28501 accgtctttg ttcagatccc aaccctgatt cgccgcccag tgtttagcaa tcaaatcgcc
    28561 ctgaataatg ccggactctt tggaatcagt gccaacgtag taggctttgt cgtagctatc
    28621 cagcgcctta cgagacggtt ctttgttgaa gaaaaccacc ggcacgtttt gcccacgcgc
    28681 tttctcaatc accgtacccg cagctgccgg gtcaaccagg ttgattgcca gtgccttcac
    28741 ccctttcgcc agcaatacgt cgatctgatc gttctgcttg gactggtcat tctgagaatc
    28801 attcatcagc agctgaacat ctggcgcggc tttcgcatct tgctcaatag ccttgcgcac
    28861 tacagacata aagttatcgt cgtacttata gattgttaca ccaatgcgag tatcagcagc
    28921 gtgtgcagcg gcaccgaata acatgctggc catcacagca gacagggtta acaccttctt
    28981 attcatggta tctccggttt ttcttatgca gggtagtgct tgagataaat gctcggcggg
    29041 gcagtagagt taatgaagtg ttactgaacg ccgaagctca ctttttaaaa ttcgttcttc
    29101 catgcccggt aacgctccag aaaacggctt taattgttgt tatgacgcta ttacctcggt
    29161 gaaagtgatt attcactgtt acatacgggt tacaacgtta aaacggtgca atcatagcta
    29221 tcacattgtt aagatactgt gaaatcactc acagattgaa agcggttaca tcgcctgatt
    29281 tgttgagtta gtgatcgtcg ccgcattctg gcgtgttgcg acagaatggc gacgcactaa
    29341 cgtcggcata aaacagtggc tagcacgagg atcaatattg cctgctgccc cctgcaaggc
    29401 cagttcggtg gctaatttcg ccattgaagc aatgggataa cgcacggtcg ttaattgcgg
    29461 gtcggtgtaa cgggcgatgg gaatatcatc gaaaccgatg attgagagat gtaacggaat
    29521 cgcaatgcca ttatctttta atgctgtcag tgcgccagca gccatattgt cgttataagc
    29581 aaatacagcg gtaagttgta gattgcgccc cagcagttca accatcgccg cctcaccgcc
    29641 cggcatgtcc ggcgtaccag tgccaatcca gctttccggc ggaataatat cctgctcttt
    29701 caacgcactc atccagcctg ctttacgcat ggcgtcatct tcaatgccgt gactggaaga
    29761 aagataacca atacgttgat gaccgttatt cagcaacatg cgcgtcgcca ttcgggcacc
    29821 gctgagatta tccaggcaaa cgcaacgatg ggcgtacccc ggcacaacgc ggttgattaa
    29881 caccataccg ggaatgttat ccataaattg cgccagttca tcgtcactca atgcttttga
    29941 gtgaacaatc aacgcattac aacgctggcg aattaacacc tcaatggcgt gacgctcttt
    30001 ttccgcttca tgatagctat tgccgattag cacgtatttc tgatgctgct gagcgaccag
    30061 atccaccgct tttaccagcg cgccgaaaaa cgcatcagaa acgtccatca ccaccacgcc
    30121 aatggtgtcg ctaacctgag ttgccagcgc ctgggcattg gcgtttggcc gataatccag
    30181 ctcactcacg gctttcatta ctgcttcacg cgtgtcggca ctgaccagcg tgctgttatt
    30241 gagcacccgg gaaaccgttg ccacagagac gccagcctga cgcgctacat cacgaatggt
    30301 gatcatattc actacctgtt atgagattgc agtaaatgac tgcttgctgg cggctatttt
    30361 gtcagcactg aatacaggac ttcgtgaatc gagtcacagc aatggaaacg gttacagccg
    30421 ttttgttaac gaatgtgatc cagatcgtta tcttgatgtt ttagatattg ccgttccggc
    30481 agcacgcaaa gttaactgac gccagagcca ttccaccggc ccctggcgga agtaacgcag
    30541 ccagataaca gagaagagga tattcgccag ccataccgga ataacaaacg ccagcagctc
    30601 caggcggtca aaatgcataa acaaaccgag gtggtaaaaa agcatggtac aaatcagcgt
    30661 ttgcaataga tagttggtca gcgccatccg tccgacgcag gcgatcgcaa gcaccagctt
    30721 aaagcggctc aattgcggcc agaagccata aaacagcgac gcatagccaa tcgcctgaaa
    30781 cggcgcactc agttcccgcg gcatttgaag taagaaggca caccagcgat acgcccagtc
    30841 cagctgccat tgcagggcga tggcaggaag gttaatggtc accccaatcg ccaccagcac
    30901 aaaaccagta cgacgatagt gacgtaagct gaactgccct ttcagccagc cgctgcgcat
    30961 cagcgctgca ccaatgagca tcatcccagc cagttgccag ccatactgtg cgcccagtgc
    31021 cagtaaactg ttgccaacac catcggcacg attactgatc gcttcaacgc cgccgtgaag
    31081 cttccagtat ttttcataca aaatagccga tgcatccggc gtccaggcgc ggctggtctg
    31141 gctgtcggaa atcagcccca ataacagcaa aacgccaagc cccaccagat aaagcatgac
    31201 gcctgtatta aacaggcttt ttaccgatgg cgcatcgcgc accagccgcc agcagattaa
    31261 gcccaccagc ccgtaagcca gcagaatatc gccgtcccag aacaataaac cgtgaataaa
    31321 gcccaataag actaacagcg ttaaccgcga ctggatccag cgtctgccac ggggcagcaa
    31381 catttgcagt ccagcaccaa acagcagcgc aaaaagcgtg aggaatttca cctgggcgat
    31441 gagatcaaga aatgcccagg tccatgcatc ctgcggcgta atagcaccgt accaggcggg
    31501 attgagataa gccgcctttg gtagcccaaa ggcgctgatg tttaatagca ggatccccag
    31561 aatggcgacg ccgcgaacaa aatcgagcgt gacgttgcgc tccatggttc ctgcctttta
    31621 atcagttgtg atgacgcaca gcgcgcagaa actcgtggcg cgtattctga ctggatttga
    31681 acaatccacc aagagaggtc gttgttgtgg cactggttgc atcgcggatg ccacgcgcct
    31741 tcacgcagta atgcaccgcg tcgatcgaga cagccacgtt attggtgccc agcagcgttt
    31801 gtagcgcaat aagaatttgc tgcgtcagac gttcctgcac ctgcggacgc tgggcaaaga
    31861 actgcacgat acggttaatt tttgacagac cgatcaccga atctttcggg atataagcca
    31921 ccgtcgcttt gccatcgatg gtaacaaaat ggtgttcaca ggtactggtc agagtgatat
    31981 cgcgcacggt gaccatttca tcgaccttca ttttgttttc aatgagggtg attttcggga
    32041 agttggcgta atccagaccg gagaaaattt catcgacata cattttagcg atgcgatgcg
    32101 gcgtttccat caaactgtca tcagccaggt cgagattcag cagctgcatg atttcggtca
    32161 tatgaccagc aataaggctt ttgcgcgttt cgttatccat ttcatgcacg ggcgggcgca
    32221 gcggtgtttc cagtcctcgc gcaactaacg cttcatgaac cagggccgct tctttactga
    32281 gtgatggcat ttatgatttc tcctgcaggt gtgacgcctc cgccctgcgt gggggcaaag
    32341 ttattaagct gatttacagc ttgattattg tgcgtgaggc ggcgcacata atccagtatt
    32401 cacagcgata attattgtaa ttgccgctgc ctttcatcag cagatgttaa aacatcgtta
    32461 tgcaaatacg gaagtgaaag ttactcacag cacattgaat aaacggtatg atgaagaaat
    32521 tgcaaacaac acaacaagga gccacgcatg gaaatgctcg aagagcaccg ctgttttgaa
    32581 ggctggcagc aacgctggcg acacgactcc agtaccttaa actgtccgat gacgttcagt
    32641 atctttctcc caccacctcg tgatcacacc ccgccaccag tgctgtactg gctttccgga
    32701 ttaacctgca atgacgagaa cttcaccatc aaggcgggtg cccagcgcgt ggcggcagaa
    32761 ctggggattg tactggtgat gccagacacc agcccgcgcg gcgaacaggt tgccaacgat
    32821 gatggctacg atttaggcca gggcgcgggg ttttatctta acgccacgca accgccgtgg
    32881 gcgacgcatt accggatgta tgattatctg cgcgatgaat taccggcgct ggttcagtcg
    32941 caatttaatg tcagcgaccg ctgcgccatt agcggtcatt caatgggtgg tcacggtgcg
    33001 ctgattatgg cgctgaaaaa tccgggtaaa tacaccagcg tttcggcctt tgcgccaatt
    33061 gtgaatccgt gcagcgtccc gtggggaatc aaagcgttta gcagctattt aggtgaggac
    33121 aaaaatgcat ggctggaatg ggacagttgc gcgctgatgt atgccagtaa cgcgcaggat
    33181 gcgatcccga cgcttatcga tcagggcgat aatgatcagt ttcttgccga ccagttgcaa
    33241 cctgcggtac tggcagaagc cgcgcgccag aaagcgtggc cgatgacgct gcgtattcag
    33301 ccgggatatg atcacagtta ctacttcatc gcctctttta tagaggatca cctgcgcttc
    33361 catgcgcagt atttactgaa gtgaaagtcc gcccggttcg ccgggcatct tctcatcaga
    33421 agcgataatc cactgccata aagtaacgac gtccgtcttc gttatagctg tagtcgtcac
    33481 gactgagatc cttgtcgcca aggttcagca cgcctgcgcg cagtttgacg tctttagtca
    33541 cctgccaggc cgcgccggta ttccagatgg tataaccgcc cggtgtttta gccgtcgcgc
    33601 tgtcggcgcg tttctgcccg gtatagtgac cagaaacata gaatgaccag tcttccagcg
    33661 ccagcggttt ccagtccagc gtaccgttag cagtatggaa cggcagatcg gatagcggtt
    33721 tgttttcgcc gttgctgaca tcacgaccat cgttgtaggt gtagttgatc gacagtttcc
    33781 attcatcgtt gaacggaatt ttcagttcgg tttccacgcc ctgaatacga gctttgttaa
    33841 cgttgtagta gctaaatacc ggtatgcgcc gtccgttagc gcccgtctca aaaccaacaa
    33901 agttttggta gcccggtgca gcgttgacgt cagacgtacg gctgatgctg atacgatctt
    33961 tcacatcgtt acggaaaacg gtaacgctgg attcaacccc ttccagccag ccttcttcac
    34021 ccatgtagta aagccccagc tcccaacttt cgctggtttc tggtttcaga tccgggctac
    34081 ccacaatctt acatgcgcca cggcaggaat tgctcgtcca gtcagggcta agttgcaaca
    34141 gagaaggtgc tttaaatgcc gtcgcccagc cccctttcac cgttacggtg tcggtggcgt
    34201 tataaaccag gtaggcacgc ggactccagt gttcaccgta ggtttcgtga tcgtccatac
    34261 gcacgccggt cgtcagcgcc agcggctcga agatccgcca ttcatcttcc acaaacagcg
    34321 cgtactggct ggcagacgtt ttggagctgg ttcccccggt caggttcacc gcatcgctaa
    34381 gtttgtcgtg acgccattca ccgccaaccg tgagaaactg attaattgcc gtcagcggca
    34441 acgtgtattt gccgtcgacc gtattgcttt cggaagttat cgggctgctg ttgccagggt
    34501 ttttgttctc gactttctca ccgtagtatt tcagttcgct ggtgccgtaa tcccaacgcc
    34561 cattatggct gacggagtag ttctggcgtt ccaggcggtt tttgtccagc gagtcggaat
    34621 cacgatcctg acggtcgaaa ccgtatccgg cagtaaaatc gtgattttga ttcggtgtcc
    34681 aggcaaattc gacattgccg tcgcggctgg agaatccttc aatacgcggc gtttctccgg
    34741 tatcggtggt cgttgagttt tgcgggtcat ccttttcacg ttttgccagg ctgccgtaag
    34801 ctttcattcc cagcacacca tcaattaatg gtccactggt aaagaactga ccgttatagg
    34861 tatcaccgcg atcgcgatgt tcctgaatgg tggtatcgac ggtaacggta cccgaccatt
    34921 tctgaccgat ttttttggtg atgatattca ctacaccgcc gagcgcatcg gagccgtata
    34981 gcgacgacat cgggccacga accacttcaa tacgttcgat ggaatcgacc gggatccagt
    35041 tcagatcgaa atcattgtgg cggaagacgg cattgcggga gttaacgcgt ttaccgtcga
    35101 cgagaatcag ggtgtagctg ctgtccagac cacgaatact aacgccctta cggttatccc
    35161 cttcgttcgt cagttgtacg ccaggcactt ctttgaggac atccttcaga ttctgtaccg
    35221 gttttcgctg caggtcttcc tgggtaatga cgctgatact ggcgggtgca tctttgaggt
    35281 tttgttcaac ggaagatgca gtgacaacca tcgtttcgcc atcatcatcg accgctaaca
    35341 caggccatgc acaagaaata gcggacaaac acagcccgac ccgtacgaaa gggttcaacc
    35401 taaacattcc atatctccat gaggtaacta cgaaaataaa atgggttatc gctcacatct
    35461 tcttcacgtc cccttgcgta cggcagcatc gcggtggact tatttgtatg caggtgatca
    35521 tcccagaaag ccggaacagc ctctgatttg tcagcctttt ttgattgcgg cgtaacgata
    35581 atgcaaacga taacaattat caatccaaat gttaaatttt atatccgcat gatttgtcag
    35641 ggaaatggaa ttgaaaaagc cctctcggtt gagagggctt agcaaggaag ggaggaaact
    35701 tatttcttat cgttctgcgg gaacttcatt tcgctgtagc gtacgaagtg agttccttta
    35761 atcagcttgt agccgaacca aataatcagg aacagcggga taccaatata cgttgccgct
    35821 acgccgcccc agtcaatggt atctttcagg aacgcttcgt agttctggcc caaagtgata
    35881 atcagacaca gaatgaatgc gaagatcggc cccagtggga agaaacctga acggtacggc
    35941 agatcgttaa tgtcgtgtcc ctgcaatacg taaccgcgac ggaagcgata gtggctaatg
    36001 gcaatcccca gccaggcgat aaaacccgtc atcccggagg tgttcagcag ccacaggtat
    36061 accgtctggt tgccaaacat ggaggtcagg aagcacagac cggcaatcac cgtcgtcgca
    36121 tacagcgcat tacgcggcac gccaccacgc gacagtttag cgaaaatgcg cggcgcttta
    36181 ccgtcacacg ccagggtgta cagcatacga gtagacgcat acataccgga gttacccgct
    36241 gacagcaccg ccgtcagaat aactgcgttc atcaccgccg ccgcagagag cagacccgcg
    36301 tgctggaaca ccagggtgaa cggactaacg ctgatgtctt taacatcgtt acgcagcagg
    36361 ctcggatcgg tgtacggaat aatcaggctg ataatcagga tcgcgaacac atagaacaac
    36421 aggattcgcc agaacacctg acgtaccgcg cgtggaatgt ttttcgccgg atcttcggac
    36481 tcgcctgcag caataccgat cagctcggtt ccctggaaag agaagccgac aatcatagct
    36541 acgccgatca tcgccgcaaa accaccagca aacggcgctt cgccgattgt ccagttgctc
    36601 cagcccgcag gctgcgcgcc tttgaagata ccgataatca tcagcacgcc aacgatgata
    36661 aagacaataa ctgtcgtgac tttgatcagt gagaaccagt attccgcttc accaaagcca
    36721 cgaactgaga tgtagttcag caggaagata acgccgagga acaacgcact ccagatccag
    36781 cccggtgtat ccgggaacca ccagctcatg accagctgag ctgcaaccag gtcaacggcg
    36841 atagtcaccg cccagttgta ccagtagttc cagcccagcg cgaagccaaa gccttcttca
    36901 acatagttct gaccgtaagt ggcaaacgaa ccggaaaccg gcatatatgc agccagttca
    36961 ccgagactgg tcatcaggaa gtaaaccatc aggccaatca gcatatacga gagcaatgcc
    37021 ccgcccggac ctgcctgaga aatcgttgcg ccagaggcaa caaaaagacc tgtaccgatg
    37081 gaaccgccaa tggcaatcat cgtcaggtga cgcgccttta attcacggcg taagcccggc
    37141 gcttctgtgg ttttagtttc ggaaaccata caaaaatgct atccatctta aaaatgaggc
    37201 gcgattgtag cagacgattg gcaatccttc cggcagaaat acacggttat aagacacctt
    37261 catgatcgcc cagggattat aagtaaagca gccaatctct tttctggcga atgcatgctg
    37321 agggcgctgc gtcacgaaat cgacacagca ccagcatgtt cttgtacagc aacttaacgc
    37381 ggtacattcg cgggatcgca atagtccaga aagcgccgta gcgcgttgga aaggtgtttt
    37441 tgccgatgat gtatacgcca caacgtacgc atcaggcgcg ggagagggac cgcaacttca
    37501 cttaatgtgc ctgcctgcaa ttgatcttta atcacccgtc gcgacaggca actaattccc
    37561 aacccatgac gcaccgcatg tttgattgcc tcggagttac ctaattccat cgccatctca
    37621 aacttcggta aatgtgacag caacagataa tcgacaatct cccgcgtgcc ggaaccgcgt
    37681 tcacgcagga tccacggcgc agcggccagc tgttctaagg tgaccggacc acgagccaac
    37741 ggcgaagtcg gcgcggcgaa aaccaccagc tcgtcttcca gccacggttc agaaatgatt
    37801 tcagtgctgt ggcacggtcc ttcaataaag ccaatatcaa cgcggaaatc cagcaccgct
    37861 tgcatcacgt cctggctgtt cccaacgcta agttcaatcg gcaactgcgg ataatcatgg
    37921 cgataacggg cgataactgc aggcagaatg tagttaccga tggtactact ggcatagata
    37981 cgaatcgcgc cgttgtcttc gcgaaacagt tgttcgattt caaccgcctg ttcaagcaat
    38041 gccaacgcac gcggatagag cagccgcccg tgttcattaa caaccagtct tttccccacg
    38101 cgatcaaaca gttgcacgcc aagctgccct tccaggtcgg tcaaggctgc gctcactgct
    38161 gattgcgaca acgccagcat caccgacgcc tgggtggttg atccactttt caatacttct
    38221 gcaaaaactt ccaactgccg gagggtgatg tgcatagtcg cttaccactt ataaagatta
    38281 attataaata tataatcaat tttattttta aaccagttag tcgtaacctt atgcccggtt
    38341 aaaggagagg gttatgacga atatcacctt acagaaacaa catcgtacac tgtggcattt
    38401 tattccgggg ttagccctga gtgcagttat caccggggtc gccctgtggg gtggttccat
    38461 tcccgcggtt gcgggtgccg ggtttagtgc cctcaccctc gcaatcttgt tggggatggt
    38521 tttaggcaac accatctatc cgcacatctg gaaaagctgt gacggtggtg tgctgtttgc
    38581 caagcaatat ttattacgtc tgggtatcat tctttatggc ttccgtctga cgttctcgca
    38641 aattgccgat gtcggtatca gtgggatcat cattgacgtg ttgacgctgt ccagtacctt
    38701 cctgctggct tgcttcctgg ggcagaaagt gtttggtctg gataagcaca ccagctggtt
    38761 gatcggtgcc ggtagcagta tctgtggtgc tgccgcggta ctggcgactg agccggtagt
    38821 gaaagcggaa gccagtaaag taaccgtggc tgttgcaacc gttgttatct tcgggaccgt
    38881 cgctattttc ctctacccgg cgatatatcc gctgatgtct caatggttta gtccggaaac
    38941 tttcggtatc tacattggtt ctactgtgca cgaagtggcg caggtggtgg cggcaggtca
    39001 tgccatcagc ccggatgcgg aaaacgcagc agttatttcc aaaatgctgc gcgtgatgat
    39061 gctggctccg ttcctcatcc tgctggcggc gcgtgttaaa cagctgtctg gggcgaacag
    39121 cggcgagaaa agcaaaatca ctattccgtg gtttgccatc ttgttcatcg tagttgccat
    39181 ctttaactcg ttccacctgt taccgcagag cgtggtgaac atgctggtaa cgctggatac
    39241 cttcctgctg gcaatggcga tggcggcgct gggtctgacc actcacgtca gcgcgctgaa
    39301 aaaagccggg gcgaaacctc tgctgatggc actggtactg tttgcctggc tgattgttgg
    39361 tggtggtgcg attaactatg tgattcaaag cgtcatcgca taaaccacta catcttgctc
    39421 ctgttaaccc gctatcatta ccgttttcct ccagcgggtt taacaggagt cctcgcatga
    39481 aatacattgg agcgcacgtt agtgctgctg gcggtctggc aaatgccgca attcgcgccg
    39541 ccgaaatcga cgcaaccgcg tttgccttgt tcaccaaaaa ccaacgtcag tggcgtgccg
    39601 caccgctcac gacgcaaacc atcgatgaat tcaaagcggc ctgtgaaaaa tatcactaca
    39661 catcggcgca aattcttccc cacgacagct atctgattaa cctcggacat ccggtcactg
    39721 aagctctgga aaaatcgcgc gatgccttta tagatgaaat gcagcgttgc gaacaactgg
    39781 ggctttcttt gctcaacttc caccctggca gccatctgat gcagatttca gaagaggatt
    39841 gccttgcgcg tattgccgaa tccatcaaca ttgcgctgga taaaactcaa ggtgtaacag
    39901 cggtgattga aaacaccgcc ggtcagggca gtaacttagg gtttaaattc gaacatctcg
    39961 cggcgattat cgacggcgtg gaagataaat cccgtgtcgg cgtctgcatt gatacctgcc
    40021 atgctttcgc tgccgggtat gatttgcgta ctccagccga atgcgaaaaa acgttcgctg
    40081 attttgcccg tattgttggc tttaagtatc tgcgcgggat gcaccttaac gatgcgaaaa
    40141 gcacctttgg cagccgcgtt gaccgccatc atagcctcgg tgaaggcaat atcggtcatg
    40201 atgcgttccg ctggatcatg caggacgacc gtttcgacgg cattccgctg atccttgaaa
    40261 ccatcaaccc ggatatctgg gcagaagaga tcgcctggct gaaagcgcaa caaactgaaa
    40321 aagcggtggc ctgatcgatg aacgacaggg aaaagcagat tcttaaaatt ttacggcgta
    40381 acccgctgat tcaacagaat gaaatcgccg atattctgca aatcagccgc tcacgtgttg
    40441 ccgctcatat tatggatttg atgcgcaagg gtttgattaa aggcaaagga tatatcctta
    40501 ccgagcagga ttactgtgtg gttgtgggtg caatcaatat ggatattcgc ggaatggcgg
    40561 atatccgtta cccgcaagca gcctctcatc caggttctgt tcactgttcc gcaggcggcg
    40621 tcggacgtaa tatcgcgcat aacctggctc tgcttggtcg ggatgtgcat ttaatatcag
    40681 ctattggcaa cgatttttac ggcgagacat tactggaaga aacgcgccgc gcgggcgtga
    40741 acgtttcaaa ctgcattcgt ctgcatggac acagcactgc aacttatctc gctatcgcta
    40801 ataagcagga agaaactatc ctggcaatca atgacacaca tattctgcaa cagctaacgc
    40861 ctcaactgct caatacttca cgggacttga ttcgccatgc tggtgtggtg ctggctgatt
    40921 gtaatctcac acctgaagcc ctggagtggg tctttaccat tgctgatgaa attccgatgt
    40981 ttgtcgatac cgtttccgaa tttaaagcga acaaagtaaa aaactggtac agccggatcc
    41041 acactctgaa accaacacaa aatgaactgg aaattttgtg ggggcaaccg attaaagacg
    41101 acaacgatcg catcagagct gtaaattcgc tgcatcaaca aggtgttaaa cgcatttttg
    41161 tttatctgaa agatgaatct gtattttgca gcgataagga tggagaacaa tttttgctga
    41221 ccgcgccagc gcatacgaca gtagacagtt ttggtgctga cgatggtttt atggcgggcc
    41281 tggtatatag ctttcttgaa ggatacagtt tccgcgacag cgcccgtttt gcggtagcct
    41341 gcgcggcaat ttcgcgcgcc agcggcagct taaacaaccc taccctgtct gccgataacg
    41401 cgctttcatt agtgccaatg gtgtaacaat gttgccggat tcgacgcttg accgcatctt
    41461 atccggctca taaattgcgc actacgctaa accaataaag aatcctgcaa tagtagcact
    41521 catcaggtta gaaagtgtcg ccgccgccag cgcgcgtaaa ccaagttggg cgatttccgg
    41581 cgcacggtgt ggcgcaaccg cagaaaacgc ccccaccacc accccgatag aaccaaagtt
    41641 agcgaaaccg cacaacgcga aagaaataat cgccacggtt ttagcatcca gagtgccacc
    41701 cgtttgcaga tatggcgaga aattgagata agcgacaaat tcattgatcg ccagcttctg
    41761 cccaatcaaa ctcccggcaa gatttgcatc actccagtca acccccatca cccacgccaa
    41821 tggggccaac aggtaaccta aaatggactc cagcgaggca tgtgcaaaac caaaccagcc
    41881 gccaacgccg ccgataatac cattaattaa cgcaatgatg gcgacaaatg ccataacaac
    41941 tgtcgctaca ccggcggcga ttttcagccc ggtcattgcc cctgtcgcag cggcttcaat
    42001 aatgcttttt ggcggtgttt cggtgaaaga gaggttatta aaggaaacct gcgaagattc
    42061 cgtagccggg cttaacaggc gggcaaacaa gatcccgcct gggatcgcca ttaacgatgc
    42121 cgccagcaaa tattccacag gtacgcccag ggcggcgtaa ccaatcattg tcgaaccagc
    42181 aatcgaggcc atgccactac aaatcgctgt aaataattca ttgcgattca gacgatcgat
    42241 aaagggtttg acgattgccg gaatttcgtt ttgcccgagg aaaatggtag tgaccgcgac
    42301 gaatgactcg atcttgctga tatttaacgc tttctggaag ataccgccga gaatgcgaat
    42361 taaaatcccc atcacaccga tgtagtagag aatactcacc agtgctgtaa cgaagataat
    42421 tgccggtaat actctgaaac caaagataaa tcctgcgcca tcaaacagcg tgtccatttt
    42481 cggcccgacc agcgaaccga agataaatgc gctacccgcg tcgctgtacg ccatcacttt
    42541 atgcacgcca aaagcgactt tttcagcgac ccaacgccct ggcggtaacc aaagcataat
    42601 gccgccaatc acaacctgta acactaacgc cgcgccaacg gtacgcaggc tgatcttctt
    42661 cttgtttact gacagcaaaa atgcaattgc cagcaatacc accattccca gaacacttct
    42721 catgacatcc atagtgatta tctcttcatg tcatattggg cgacggcttt ctgcccatca
    42781 gaaatagccg ggcctgcatc ttgcgctatc ggcccggctt gttatcaacg cttaatgggt
    42841 tttgatgtag ccgcgcacgc actcttcgac taagccccag aaccagtcgg tatcaatagt
    42901 gatgccgact ttggtattgg cgggcttgcc aagaacgccc agctcgtcgc agacggtgcg
    42961 cccatagcaa gggccactgt tgacgtccac ttcgacgtac atctcctggg ttttaatgcc
    43021 atcagggtta atcagataac cgatgcaggt ggcgtcgtgc accgggccgc cagccaggcc
    43081 gtagttttcg aactgcgttt tgagagtgaa gttcatgatg tcgctgaaca gctctccggc
    43141 agggccgcct gccctttcca tccgagcaat cacgtccggg gtgcaaacgg tctggttggt
    43201 gagatcgagg cccatcatca ctaatggaac gccagaggtg aacaccacgc gggctgcttc
    43261 cgggtcagca aagatgttga actcggcaga tggcgtgaag ttgcctgtac cgtaagcgcc
    43321 gcccattagc acaatttcac ggattttggg caggatcgcg ggttgcatac gcattgccac
    43381 cgcgatattt gaaagcggac caaccggcac cagagtgata tcgccatcgc ttgccatcag
    43441 ggtatcaatg atgtatttca ccgcgtgggt actttctgcc tggcgggtta gcggctcgaa
    43501 taccgggcca tccagtccgg tttcaccgtg aatattatcg gcaacgattt gttgacgcat
    43561 aatgggttgc ggcatccccg cgtaaaccgg aacattaatc tccagtttct ggcaaacatt
    43621 caggccatta attaatgttt tatcaagcgt ctgattaccc gctacaatag tgatgcctaa
    43681 taaatctatt gccggatgtt tcgctgccat catcattgca atagcatcat catgacccgg
    43741 atcacaatcc agaataattt ttctcttttc cattgtttat ttcctctgtt tccagttgcg
    43801 ttattttttc tacagcaaag aaaattcgac cgggccgatg attgaatctt accaaccgcg
    43861 tacgtatgct aaatatgaga aatctcatag cggataaaca tcgtgaaaga aatccacaat
    43921 aatgatctta agcagcaatt gatgagtgaa tccgcgttta aggattgctt tttaacggat
    43981 gtttcagccg atacgcggct gtttcatttt ttagcgcgtg actacattgt gcaggaaggg
    44041 caacagccgt cctggctgtt ttacctgacg cgaggccgcg ccaggcttta cgccacgcta
    44101 gctaatggtc gcgtgtcgct gatcgatttc tttgccgccc cctgttttat tggcgagatt
    44161 gagttaatcg acaaagacca tgaaccgcgt gcggtgcagg ctattgaaga gtgttggtgc
    44221 cttgcgctcc ctatgaaaca ttaccgcccg cggttattaa acgacacgct atttttacga
    44281 aaactctgcg tcaccttaag tcataaaaat tatcgtaata ttgtttcttt aactcagaat
    44341 caatcatttc cgttagtgaa tcgcctggca gcatttatat tactctcgca ggaaggtgat
    44401 ctttatcacg aaaagcatac gcaagcggca gagtatttag gcgtttctta tcgacatctt
    44461 ttatatgttc tcgcgcaatt cattcacgac ggtttattaa ttaaaagcaa gaaagggtat
    44521 ctaattaaaa acagaaagca gttgtcagga ctggcgctgg agatggaccc ggagaataaa
    44581 ttctccggga tgatgcagta aaaattattt gcaatagcgc gattgccgga tgcaacactt
    44641 aataagcgcg tacatccggc aatgaaactc aagctaaacc aataaagaac ccggcaatgg
    44701 tcgcactcat caggttggaa agcgttgctg ctgccagcgc ccgtaaacca agctgggcga
    44761 tttccggcgc gcgttttggc gaaatagccg aaaatgcgcc aacgacaaca ccgatagaac
    44821 caaagttagc aaaaccacaa agcgcaaagg agataatcgc aatggttttc acttccagcg
    44881 tgccgcccgt ttgcaggtat ggggagaaac tcaggtaagc gacgaattcg ttaatcgcca
    44941 gtttctgccc aatcaggcta cccgcaagat tggcatcact ccagtccaca cccatgatcc
    45001 acgccagcgg tgccagcaca tagccaaaaa tactttccag agaggcattg gcgaaaccaa
    45061 accagccacc aattccgccg ataatgccgt tgatcagcgc aataattgcg acaaacgcca
    45121 ttaccaccgt cgctacacca gcggcgattt tcagcccggt catcgcaccg ctcgccgccg
    45181 cttcgataaa gctttttggc ggcgtttcgc tgaacgacag attttcaaat gtgacctgtg
    45241 aaggctcggt ggccgggcta agaatacgtg caaacaaaat accacctggg atcgccatca
    45301 gcgatgccgc taacaggtag tcaattggta cgcccattcc ggcataacca atcatcatcg
    45361 aaccagcaat ggacgccatc ccgctacaaa ttgcggtaaa caactcgttg cgattcatgc
    45421 gatcgataaa cggtttaacg atcgccggga tctcattttg cccgaggaaa atagttgtga
    45481 ctgcgacaaa agattcgatt ttgctgatgt taagagcttt ctggaaaatg ctgccaagga
    45541 tgcgaatcag cagccccatc acgccaatgt agtacagcag actgatgagc gcagtaacga
    45601 aaataatcgc cggaagtacg cgaaaggcga agataaaacc cgcaccgtca aacaggacat
    45661 ccattttcgg cccaaccagc gaaccaaaaa tgaaggcgct accggcatca ctgtaagaca
    45721 tcactttatg aacgcctaat gccgcctgtt ctactgccca ctttcccggt gggaagtaga
    45781 gcatgatgcc accaatagcg atttgcagca gcagtgcggc tccaaccgtg cgcaaactga
    45841 tgctcttttt attcactgac aacagaaatg ctattgccag taacaccacc atccccacaa
    45901 cacttctcat tatatccata atgattttcc cttcatgcca gtaaacccgg cgtcagcgcc
    45961 aggttttggt atgcttgatg agtacgggcg acggctttct gcccgtcaga aattaacccg
    46021 cgagacgctg gtattctttg gcaatttcac tcgccagaat ggcgttgttg aacaccagct
    46081 ggatattgga tttcaggctg tcaccgccgg tcagctcagc aacgcgcgcc agcaggaacg
    46141 gtgtactttc tttaccaata acgccctgct cttcggcttc ggcgacggct tgatctatgg
    46201 ctgcattgat agattcctcc ggcatcgcaa actgttccgg gatcggattc gccactacca
    46261 ggccgccgtt caaaccggtt tgccatttca ctgccattgc acgagcgatt tccgtggcac
    46321 tatcaaggcg aatactgact tcaaatgggc tggtacggca gaaaaacgca ggcagcgctt
    46381 ttgtctgata gccaattaac ggcacaccga aggtttctaa atattctgtt gttaatccta
    46441 aatcgagaat agatttcgcc ccggcacaaa caacggtgac attagtattt gccagttctt
    46501 gcaaatcggc agaaatatcg aaggtatgtt ccgccccgcg atgcacacca ccaattcccc
    46561 cggtggcaaa tactttaatt ccggcaagcg ccgcaataat catcgttgaa gccacagtgg
    46621 ttgcgccatt ttttccggcg gcaacaacaa aaggtaaatc gcgacgacta actttggtca
    46681 cgttatgccc ttcacgaccc agtaattcaa tttcttcttt gcttaaaccc actttcatca
    46741 cgccgccaat aatggcgata gtggcaggta ctgcgccctg tttacgaata gtttcttcta
    46801 cttcaattgc ggtctgggca ttttgtggga acggcatccc gtgagaaata atggtcgatt
    46861 ccagcgccac aaccggtttt ttgtttttta aagcgtcctg cacttccggg gaaatttgta
    46921 ataattcagg ggaaattttt aattcagaca ttctgcgttc tccactaacg atataacgtt
    46981 ggcaatcgat aaatcggggt tattggtgta ttcacaggag agcgccattg acgaacatcc
    47041 ctgtgcgaaa cgaacagatt cggcaaacgg cattccgtct acccaacacg aagcaagtcc
    47101 cgccatcatg gcatcgcccg ctccggtaac attaataaca ttggttttga tcggggcaga
    47161 ccagccactt tcaccgctga tatcactgta ataaacgcca tcgccgccca tgctcaatac
    47221 cagtcggttc aggccatgtt gatggaacca ggcagcaact tttgccacat ctccacgccc
    47281 tgacagcgca atcccactca gtgtttccgc ttcaaggcgg tttggcttca gagtgtggat
    47341 ctgatttaga cggtcgcgca ctttgacaca tttccacgct gaaaccggat cgacaaatac
    47401 gggtacgttg gcagcattat ccagaatcca tgccagtgcc tcttcactga tattacagtc
    47461 cgcgacaatg acctttgccc tctgaataaa ttcacggtgc tgtgcgagat attcagctgt
    47521 aatagcgttg ctaatattca tgtcatttat agcaaccagc atttcaccgg tattatcgag
    47581 taatgataaa taactcgacg tattttctcc cggcacaatc aggcatttat cgacataaac
    47641 gccagattga ttggtttgcg ttagcagcga ttgaccataa aaatcactac ctactgcgct
    47701 cagtaaccag gctttgttac ccagcaacgc caggttttgt gcaatatttc gccctactcc
    47761 accaggtgta aattttattt tacctggatt tgaatccgca taatttaatg attcatgtga
    47821 atatccggcg acatcaatat tcgccgaacc tataattacg acataatcct tttcgcgcat
    47881 agcacgtcct ctggtaaaaa attaccaacc ctgtcaatcc acaagaatat agatactgca
    47941 aagacagttt aaacatgtgt tcatatttaa acatgtgctc atagtatgta tgttttacgt
    48001 aaagtaaatt gccgccagag aggcaaagcg ggcagatttg agcaggctca caaaatgcga
    48061 ttaaatgcca gaattacaaa agcgaggaat ttgtgacggg atgcacagaa caggaacact
    48121 aaaaaagggc agaaaagaat ctgcccttga ggaaataacc ttagtgtttg atgaacgttt
    48181 caggtttcta ctgaaagata aaaccattta aattcataaa attacattca caaaccggtc
    48241 attctctttg cgctcatcca atgatacagg gcagtaaatt tctgccctgt aacacacctt
    48301 ttattacgcg gctttcgcta ctgcgtccac ttccggacgt ttcaggaagg cataggccaa
    48361 acccgccacc agcgtaccgg caataattgc taccaggtaa cccagtaccg gcgtaatagc
    48421 gccagggatc agcagaacaa acagaccacc gtgcggtgcc atcagtttcg caccaatcgc
    48481 cattgagatt gcgccagtca gcgccccacc aacgatacaa cacggcagca cacgcatcgg
    48541 atcacgagca gcaaacggaa ttgcaccttc cgaaatgaag cacagtccca ataccagagc
    48601 ggctttgcca ccttcctgct gcgctttgtc gaatttgcga cgcgccacca ttgttgccag
    48661 acccattgcc agcggtggca ccatacctgc cgccataatc gccgccatcg ggccataggt
    48721 ttgagtactc agcagaccca caccgaatgc gtacgctgct ttgtttaccg gaccgcccat
    48781 gtcagtacac atcatgccac cgaggatcgc ccccagcaga accgcattcg cagtccccat
    48841 ggtctgcagc cagtgagtca acccttcgag aatgccagca actggtttac cgatcaggta
    48901 gatcatcgcc agaccgacca ccagactgga aattagcgga atgatcagga tcggtttcag
    48961 cgcctccata ctctgtggca gtttcagttg cgtactgatt aacttcgcaa tgtaaccagc
    49021 caggaagccc gcaataatac cgccaatgaa gccagaaccg gtgctgaccg ccagcatacc
    49081 gccaatcaga cccggcgtga gacccggacg atcggcaatg gagaaggcaa tgtaacctgc
    49141 cagtaccggc accatcagcg caaaggctga accaccacca atctgcatca gcgccgctgc
    49201 caacgtaccc ggctctttaa acgcttcgat accaaaagca aaagaaagcg cgatacacag
    49261 accacctgca acgaccatcg gcagcatgta agagacgccc gtcagcaagt gacggtatgc
    49321 gcctgcactc tctttcttac cttcagaggt cgccgtttga gctttgcccg ccggttcata
    49381 cggcgttgct tcagcaaccg ctttatccag ttcctgcgcg gttttcttca gcgccagacc
    49441 ggtagaggta cgatacatcg gtttaccagc aaatttcgcc agatccactt cgatatctgc
    49501 cgccacaatc accagatccg ctgcggctac ttcttcgggg gtgattgcat tacccgcacc
    49561 aacagaacca cgggtttcaa ctttcaccca ccagccacgt tttttcgctt cggtttcaat
    49621 ggcttcagcc gccataaagg tgtgtgctac gccagtcggg caagcagtca ccgcaactac
    49681 gcgtttcgga ccgctggcgg caaccggtgc tgtcgcagcg accggcgcag tgtaaggttt
    49741 cgcatggcct ttggcttcac tcaggaacag ctcagggtgc gcaactgccc gggaaatatc
    49801 acccagccag acatttttac cgttcagcgc gctgtcattc gggatggaat caccgagaac
    49861 aatcgccatt tctgcgtcgt tcggattgtc gatgatttcc agttttgctt ttcgcgccgc
    49921 cgcgcccagc agggtcttcg ccatataggc gcgtgcctga ccgagattag cgtcaataat
    49981 cagcagcgtt ttcattatgc ctctcctgct gtcagttaaa aggttgtaag tcgacgcgcg
    50041 ccatcattgc ggccaactgc ggacgatcgg taatacccac attgctttga cttaccgcca
    50101 gggctgcaac agcagtcgcc agacgcagtg tgtgttcact ggattcacgc atcagcaagc
    50161 cataaatcag gccaccaacc atagaatccc ctgcgccaac ggtgcttacg acatcgactg
    50221 acggtggttt ggcgatccat tcgccggagg cattaaccca aagcgcgcct tcggcaccca
    50281 gtgaaataac aacatgcgca ataccttgtt cacgtagcgc atgtgcagct tcaatcacat
    50341 ctttcatttc aggcagttta cggcctgccc agatttccaa ctcgcggcgg ttaggtttca
    50401 ccagccacgg tgccgctttc aaacctgcta ctaacgcttc acggctacta tcaaagataa
    50461 tgcaaggaca ctgactacgc aggcgagtca tccagtcggt gaacgcttcc gggctgacgc
    50521 ctgacggtaa gcttccgctg acacagacca tatcgaactg accgagccag ctcagagaat
    50581 cagtcacaaa gcgttcccag tcggcggggg tgacttcaaa acccgagaag ttgaagtcgg
    50641 tcacttcgcc gtctttttcc gtcagcttaa cgttaattcg ggtgcgcccc tgtacaacct
    50701 ggaaacggtt ggcaatgccc agctcgctga acagttgctg aaaaccatcc tgattgtctt
    50761 tacccaggaa gccgccaacg gtgacatcaa ttcccaggtc ttttaatact ttggccacgt
    50821 tgatgccttt acccgccgca tgcagaccgg tggttttcac caggttcact tcgccgcgtt
    50881 caatttccgg gcagaaacca acaaggtcat aagccggatt aagggtgata gtagcaacac
    50941 gtctgctcat tatgcgccct ccccaagacc agcagcgata gcgtcgccga ttgctttcag
    51001 cgcctgttca gcatctgcac cctgggcggt aaagcgtagg cgatgacctt tcttaacgcc
    51061 aagtgccaca actttcatca gactacgtcc gtttgccggt ttgccggtgc catcaaggtt
    51121 tgtcacggta atatcactgt taaattgttt aatggtgttg accagcatgg tacctggacg
    51181 agcatgcagg ccgtgttcat tgcgcaccac aaactccgcg cttaacacgt cgtcggtcgg
    51241 cgcatcatcg ctggtcagca gcgccagcaa cgttgccgca tccgctttca gcaagcggtc
    51301 agctttattg tcgagcaaca aatcagcgag acgcttaaga accgcaatgg gctgatcgtc
    51361 attcatcgcc acactcacca gcatggctgc cgtttcgccg tccacatcaa aagcatttgc
    51421 cgcacggctt accgcaatcg cgctacgcag attgccttcg gcgctatcgc tcagccagat
    51481 accctgtccg aggttcagcg gttgttcatt gatggctttg gtgacgaaag tggcgtcaac
    51541 tgccctcgcc tctttcagac gcgcagcgtt cagcgcctga agagtcagca gatcgctggc
    51601 ggcgatatcc agcgtcagca tttcgttgtc gagcttcagc tgctcactct gcttttcgcc
    51661 catcagcaat gcgcgaagtt cttctgctgt tgttgctgac ttcagttgtt cagcaacgga
    51721 atcatcgctc agtacgtggg tcagctggcg tagcaggccc agatgttcat ccgagctggc
    51781 agcaataccg atcgctacgt acgctacctg accgtcaccc caggtgacgc cttccgtgaa
    51841 ctgaaatacc tgaacgccgg ttttcagcac ctgatcgcgg gtgtcggtag tgccgtgtgg
    51901 aatagcaata ccattgccga ggaacgttga ggtttgctgt tcgcgcgcca gcatgccatt
    51961 gacgtagcct tctgctacgt taccggcctg caccagcgcc gcagcgagct ggcgaatcgc
    52021 ctcttctttg tctccggcct tttcgcccgg atggatgtcc tgtacggata actggaacat
    52081 agttctcctc tcttgctgaa ttgaaacgat tcagcttcta tgagaaaaaa agcgccaacc
    52141 tggcttaggg ttaaagacaa gatcgcgctg aaacgtttca agaaagcata atacttctgt
    52201 ttcagcacgc aaggaaagcc gtaaagttat gagcaaaagt ttgatgtgct gcacaatttt
    52261 ttgtcatttt tcctaattgc tgatgggaaa atccgttatc agcaatttca tttcagcagc
    52321 gtcagcagcc aatcagcaac agtgaaatgg cattttgatt attcgtggcg cttttgattt
    52381 cgctgtctgt ttattttctg acaagcagcg taaactccgc gtcttcctct tccagtgatc
    52441 gaccagcatg cataactccc ccgcagtctc cagcgcgaaa tcgtttgacc tgacctcgac
    52501 ggcgttttta atcgttgcct ttctcaccgg tattgcgggc gctctgcaaa ccccgacact
    52561 cagtattttt cttaccgatg aagtacatgc ccgtccggcg atggtgggat tcttctttac
    52621 cggcagcgct gtcattggga ttctggtaag tcagtttctc gccgggcgct ctgataagcg
    52681 cggcgatcgc aaatcgctga ttgtcttttg ctgcctgtta ggcgtgctgg cctgcaccct
    52741 ttttgcctgg aatcgcaact actttgtttt gctattcgtt ggcgtctttc ttagcagctt
    52801 tggctcgacc gctaacccgc aaatgtttgc ccttgcccgt gaacatgccg acaaaaccgg
    52861 acgtgaggcg gtgatgttca gctctttttt acgcgctcag gtttcactgg catgggtcat
    52921 tggcccaccg ctggcttatg cgttagcgat gggtttcagc tttacggtaa tgtatctgag
    52981 cgcagcggta gcgtttattg tttgcggcgt gatggtgtgg ctgtttttac cgtcgatgca
    53041 aaaagagctt ccgctggcga ccggcacggt tgaagcgccg cgccgtaacc gtcgcgatac
    53101 gctactgctg tttgtcattt gtacattaat gtggggctcg aacagcctgt acatcatcaa
    53161 catgccgcta tttattatca acgaactgca tcttcccgag aaactggccg gtgtgatgat
    53221 ggggaccgcc gccgggctgg aaatcccgac gatgttgatt gccggatatt tcgccaaacg
    53281 tctgggtaag cgtttcttaa tgcgcgttgc tgccgtgggt ggcgtctgtt tttacgcagg
    53341 aatgctgatg gcgcattcac ctgtcattct gttgggcttg cagctgctaa atgctatttt
    53401 tattggcatt ctgggcggca tcgggatgct ctattttcag gatctgatgc ccggtcaggc
    53461 gggttcagcc accacgctct ataccaacac ttcgcgcgtg ggctggatca tcgcaggatc
    53521 agtggcgggc atcgtcgccg agatctggaa ttatcacgct gtgttctggt ttgcgatggt
    53581 gatgattatc gccactctgt tttgcttact gcggattaaa gatgtttaag gcgcggtcag
    53641 catttccaga tcgataagcc aggtcatggc ctgctgacgc gagttaccgc acatttcagc
    53701 gctggcttgt aagcctgcac agactttcgg tcgcagtggc gaggtgaaaa ttttacagcg
    53761 ttgctgttca tcaagctgaa tgcagggcgt attggcgggc ttgccatctg gcataccggg
    53821 aatagggctg gaaattgaag gggcagtgca acacgccccg caacccggac ggcattccat
    53881 acgttgttct cttaaggtta accaccggat tgatgagcgc gcacagtacc accttttttg
    53941 caccagcaaa agtgcgaata ccacttgccc gaaaggcccg tcgcgagtac tttgtcgcga
    54001 tatttttgac attttcgact acaggaattt ttcgatgcca agagcgaacg aaattaaaaa
    54061 aggtatggta ctgaattaca acggcaaact gctgctggtt aaggatattg atattcagtc
    54121 gcccactgcc cgcggtgccg ctacgctgta caaaatgcgt ttttctgatg tccgtaccgg
    54181 gctgaaagta gaagagcgtt tcaaaggtga tgatatcgtt gacaccgtga cgctgacccg
    54241 ccgttacgtt gacttctcct atgtcgatgg caacgaatat gtctttatgg ataaagaaga
    54301 ctataccccg tataccttca ccaaagatca gattgaagaa gagttgctgt ttatgccaga
    54361 gggcggcatg ccggacatgc aggtgctgac ctgggacggt caactgctgg cgcttgagct
    54421 gccgcagacc gttgatctgg aaatcgttga aaccgcacca ggcatcaaag gggcatctgc
    54481 cagcgcccgt aacaaaccgg cgacattgag cactggtctg gtgattcagg taccagaata
    54541 cttaagcccg ggcgaaaaaa ttcgtatcca tatcgaagaa cgccgttata tggggcgtgc
    54601 tgactaactt cagccgcatg cagaaaaggg atagctcagg ctgtcccttt tttaatttat
    54661 tataccaatc ttctattttg cgcttcctga acaccttacg ccctcaattt tcactcgttg
    54721 attgatcgcc ctcacacttc atcgcattaa caatccagac caatttcaat tgctgtcata
    54781 taactttaca ctgtcattgt taatttatcg ttactaagac gtgactccta tgaagacaat
    54841 tgcctccgtt acgctcccgc atcatgtaca cgctccacgc tacgatcgcc agcagttgca
    54901 atcacgtatc gttcattttg gctttggcgc ctttcaccgc gctcatcagg cgttactgac
    54961 cgatcgtgtg ctgaatgccc agggcggcga ctgggggatc tgtgaaatca gcttgttcag
    55021 cggtgatcaa ctgatgagcc agctccgcgc acagaaccat ttatataccg tgctggagaa
    55081 aggtgcggac ggcaatcagg tgataattgt cggtgccgtt cacgaatgcc ttaatgcaaa
    55141 actggattcc ttagcggcaa ttattgagaa attttgcgag ccacaggtgg caattgtttc
    55201 cctgacgatt accgaaaaag gctattgtat tgacccggcc accggtgcac tcgacaccag
    55261 taatccgcgg attattcacg atctacaaac ccctgaagaa cctcactccg caccgggtat
    55321 tctcgtcgaa gcactgaaac gccgccgtga gcgcggcctt acaccgttta ccgtgctctc
    55381 ctgcgacaat attcccgaca atggtcatgt ggtgaaaaac gcggtgctgg gaatggcaga
    55441 aaaacgttcg ccagaactcg ccgggtggat aaaagagcac gtcagttttc cgggaaccat
    55501 ggtcgaccgc attgttccgg ctgcaaccga cgaatcactg gtggaaatca gccagcatct
    55561 gggggtgaat gatccctgcg cgattagctg cgaaccgttt atccagtggg tggtggaaga
    55621 taacttcgtc gctgggcgtc ctgcctggga agtcgcaggt gtacaaatgg tgaatgatgt
    55681 cctgccatgg gaagagatga aactgcggat gcttaatggc agccactctt ttctcgctta
    55741 tctgggttac ctctcaggat tcgcccatat cagtgattgc atgcaggatc gcgcatttcg
    55801 ccatgccgcc agaacattaa tgctggatga gcaagcgccg acactgcaaa ttaaagatgt
    55861 cgatttaaca caatatgcgg ataagttaat tgcacgtttt gctaatccgg cgctgaaaca
    55921 taagacctgg caaatcgcga tggatggcag ccagaaatta ccgcaacgca tgctggcagg
    55981 tattcgcata catcaggggc gcgaaacgga ctggtcgttg ctggcattag gcgttgcagg
    56041 ctggatgcgt tacgtcagcg gcgttgatga tgccggaaat gccattgatg ttcgcgatcc
    56101 gcttagcgat aaaattcgcg aacttgttgc gggcagcagc agtgaacaac gcgtaaccgc
    56161 cctgctttcc ctgcgtgaag ttttcggtga tgatctgcca gataacccgc attttgtgca
    56221 ggccatcgaa caagcctggc aacaaatcgt acaattcggc gcacatcagg cgctattaaa
    56281 caccctcaaa atttaacgat ttctgcggtt aaagcggatg aagctcacct tcgtccgctc
    56341 tccccttctc ttttctgctt tttttagcca ggattaacgc tcagttaact taccagagta
    56401 ttgcggagcc gttgtgacca ggaccaacct catcaccggt tttctcggca gcgggaaaac
    56461 cacgtcgatt cttcatctgt tagcccataa agatcccaac gaaaaatggg cggtactggt
    56521 taatgaattt ggggaagtcg gaattgatgg tgctttgctc gccgatagcg gcgcattgct
    56581 gaaagagatc cccggcggct gcatgtgctg cgttaatggt ttacccatgc aggtagggtt
    56641 gaatacctta ctgcgtcagg gaaaaccaga ccgcttgttg atagagccga ccgggctggg
    56701 ccatccgaaa cagatcctcg atctgttaac cgcaccagtc tatgaaccgt ggatagatct
    56761 gcgcgccacc ttgtgcattc tcgatccgcg cttgctgctg gacgaaaaaa gcgccagcaa
    56821 tgaaaacttc cgtgaccagc tggctgccgc agacatcatt gtcgccaata aatccgaccg
    56881 tgcgacgccc gaaagtgagc aagcgctaca gcgttggtgg cagcaaaatg gtggagatcg
    56941 acaattaatt cacagtgagc atgggaaagt tgacggtcat cttctggatt tgccgcgtcg
    57001 caatttagcc gagttgcccg ccagcgccgc gcattctcat cagcatgtcg tgaaaaaagg
    57061 gttagcagcg ttaagcctgc cagagcatca acgctggcgt cgcagtctga acagcgggca
    57121 aggatatcag gcctgcggct ggatattcga cgctgatacg gtattcgaca ccattggcat
    57181 tctggaatgg gcgcgacttg caccggtgga acgcgtcaaa ggcgtgctgc gtattcccga
    57241 agggctggta cgaatcaacc gtcagggcga tgacctgcac attgaaacgc aaaacgttgc
    57301 gccaccggac agccgtattg agctgatttc cagcagcgaa gctgactgga atgctttgca
    57361 gagcgcgctg ttgaagcttc gtttagcgac taccgcgtaa ggttgcctgc gtttttcagt
    57421 aagataatta gagaaaatat gattaaaaat ttgccgcaaa tagtgttgtt gaatattgtc
    57481 ggcctcgcgc tgtttctttc ctggtatatt cccgttaatc atggattctg gttgccgatt
    57541 gatgcggata ttttttattt ctttaatcag aaactggtcg aaagtaaggc ctttttgtgg
    57601 ctggttgcat tgaccaacaa tcgcgctttc gacggttgtt ctctgctggc gatgggaatg
    57661 ttgatgctga gtttctggct gaaagaaaac gcccctggca gacgacgtat cgtgattatg
    57721 ggtctggtca tgctattaac tgcagtggta ttaaaccagc tgggtcaggc attaattcct
    57781 gtaaaacggg ccagcccaac attgactttt accgatatta accgcgtcag cgaactgctc
    57841 tctgttccca cgaaagatgc ctcacgagat agctttcccg gcgatcacgg catgatgctg
    57901 cttatttttt cggcattcat gtggcgttat ttcggcaaag ttgcaggcct tatcgccctt
    57961 attatttttg tggtttttgc atttcccaga gtaatgattg gcgcacactg gtttactgac
    58021 atcattgtcg gttcgatgac cgtgatattg atcggtttgc cctgggtgtt gctgacgcca
    58081 ttaagtgatc gattaatcac cttttttgac aaatcactac cgggaaaaaa caaacatttc
    58141 caaaacaaat aactcacagt aattaacatc atcaggctta tttttatagt gaggataatc
    58201 ctgatgatgc gcaccgtgct ttcatccatc gcgcgcaaaa atcattctct aagtaaatga
    58261 atggattgca tgcgtttcac tcaatcgtac tttaattgac caatcccgct tattaacttt
    58321 ctgtatcact ttttcttata aaaaatcatg taaaaccgct cgccaagacc gcaccaatcg
    58381 ggtaatctcg aactcgtttt gcctcggcgg tagattatcc tcacagcata taattttgtg
    58441 cgttagtcca cagatttggc cttaaggaat tgtttcaaca tgcccaggta attagtctcg
    58501 tgtcgcttgg cattttttta taacgatatt tgtcgttaag gacttcaagg gaaaacaaac
    58561 aacatggtca aatctcaacc gattttgaga tatatcttgc gcgggatccc cgcgattgca
    58621 gtagcggttc tgctttctgc atgtagtgca aataacaccg caaagaatat gcatcctgag
    58681 acacgtgcag tgggtagtga aacatcatca ctgcaagctt ctcaggatga atttgaaaac
    58741 ctggttcgta atgtcgacgt aaaatcgcga attatggatc agtatgctga ctggaaaggc
    58801 gtacgttatc gtctgggcgg cagcactaaa aaaggtatcg attgttctgg ttttgtacaa
    58861 cgtacattcc gtgagcaatt tggcttagaa cttccgcgtt cgacttacga acagcaggaa
    58921 atgggtaaat ctgtttcccg cagtaatttg cgtacgggtg atttagttct gttccgtgcc
    58981 ggttcaacgg gacgccatgt cggtatttat atcggcaaca atcagtttgt ccatgcttcc
    59041 accagcagtg gtgttattat ttccagcatg aatgaaccgt actggaagaa gcgttacaac
    59101 gaagcacgcc gggttctcag ccgcagctaa taaaccgttt ggatgcaatc ccttggctat
    59161 cctgacgagt taactgaaag cactgcttag gcagtgcttt tttgttttca ttcatcagag
    59221 aaaatgatgt ttccgcgtct tgatccaggc tatagtccgg tcattgttat cttttaaatg
    59281 ttgtcgtaat ttcaggaaat taacggaatc atgttcatac gcgctcccaa ttctggacgt
    59341 aagctcctgc ttacctgcat tgttgcaggc gtgatgattg cgatactggt gagttgcctt
    59401 caatttttag tggcctggca taagcacgaa gtcaaatacg acacactgat tatcgacgta
    59461 caaaagtatc tcgataccta ttttgccgac ctgaaatcca ctactgaccg gcttcagccg
    59521 ctgaccttag atacctgcca gcaggctaac cccgaactga ccgctcgcgc ggcgtttagc
    59581 atgaatgtcc gtacgtttgt gctggtgaaa gataaaaaaa cattctgttc atctgcgact
    59641 ggtgagatgg acattccact caatgaattg attccggcgc tcgacattaa taaaaacgtc
    59701 gatatggcga tcttacccgg cacgccgatg gtgccgaaca aacccgcaat cgtcatctgg
    59761 tatcgcaacc ctttgctgaa aaatagcggt gtgtttgccg ctctgaatct caacctgacg
    59821 ccttctctct tttatagttc acggcaggaa gattacgatg gcgtcgccct cattattggc
    59881 aatactgcgc tatctacctt ttcttcacgt ttgatgaacg ttaatgaatt aaccgacatg
    59941 ccggtccgtg aaactaaaat tgcgggcatt cctctgaccg ttcggcttta tgcggatgac
    60001 tggacatgga acgatgtgtg gtacgcattt ttactgggcg gcatgagtgg aactgtcgtt
    60061 ggcctgctct gctattacct gatgagcgta cgtatgcgcc ccggcagaga aatcatgacc
    60121 gccatcaagc gcgaacaatt ttacgtggcg tatcaaccgg tggtggatac acaagctttg
    60181 cgagtaacgg gcctggaagt actgctacgc tggcggcatc ctgtcgcggg agaaattccc
    60241 ccggatgcct tcattaactt tgccgaatcg caaaagatga ttgtgccgct gactcagcac
    60301 ctgtttgagt taattgcccg cgatgccgca gaattagaaa aagtgctgcc agtaggcgtc
    60361 aaatttggta ttaacattgc gccggaccat ctgcacagcg aaagctttaa agcagatatc
    60421 cagaaactgc tcacttccct acccgcacac catttccaga ttgtgctgga aattaccgag
    60481 cgcgatatgc tgaaagagca agaagccaca caactcttcg cctggctgca ctcggtcggc
    60541 gtagaaattg ctattgatga cttcggcacc gggcacagcg cgcttatcta tcttgagcgt
    60601 tttacgctcg attatctgaa aattgaccgt ggatttatca acgccatcgg tacggaaacg
    60661 atcacttcac ccgtacttga cgcggtgctg acgctggcga aacgtctcaa tatgctgaca
    60721 gttgctgaag gggtcgaaac gccggaacaa gcgcgatggc taagcgaacg cggcgttaat
    60781 ttcatgcaag gctactggat tagtcgcccg ttaccgctgg acgattttgt ccgctggctg
    60841 aagaaaccgt atacgccgca gtggtaaggt gtgcttacgt cccttattat tcatagtgaa
    60901 agcatgccgg attgcggcta atgatgagta aaaggaaatc cgttgcagat gattgtgcgc
    60961 atactgctgc tgtttatcgc tctgttcacc tttggtgtgc aggcgcaggc tatcaaggaa
    61021 agctatgcct ttgccgtgct gggcgaaccc cggtacgcgt ttaatttcaa ccattttgat
    61081 tatgtgaacc ccgccgcgcc aaaaggtggg cagataacgt tgtcagccct cggcaccttc
    61141 gatactttca accgctatgc actgcgcggc aacccgggcg cacgcaccga gcagctgtac
    61201 gacacgctat ttacgacttc cgatgacgaa ccaggcagtt attacccgct gattgctgaa
    61261 agcgcacgct atgctgacga ttattcctgg gtggaggtcg ctattaatcc gcgcgcccgt
    61321 tttcatgatg gttcgcccat tactgcccgc gatgtagagt ttacttttca aaaatttatg
    61381 accgaaggcg tgccgcaatt tcgtctggtc tacaaaggca ccaccgtcaa agccattgcg
    61441 ccgttaaccg tgcgcattga gttagctaaa cccggcaaag aagatatgtt gagtctgttt
    61501 tcgctgccgg tatttccaga aaagtactgg aaggatcaca aacttagcga cccgctcgcc
    61561 acgcctccgc ttgccagtgg tccgtaccgc attacgtcct ggaaaatggg gcaaaatatt
    61621 gtctattccc gcgtgaaaga ttactgggca gcaaacttac cggtaaaccg cggacgctgg
    61681 aatttcgaca ccattcgcta cgattattac ctcgatgata atgtcgcctt tgaagcgttt
    61741 aaagcaggtg cctttgattt gcgtatggaa aacgacgcca aaaactgggc cacgcgttat
    61801 accggtaaaa atttcgataa aaaatacatc atcaaagatg agcaaaagaa cgaatcagcc
    61861 caggatacgc gctggctggc gtttaatatc caacgtccag tattcagcga tcgccgggtg
    61921 cgggaagcaa tcactctcgc ctttgacttt gaatggatga acaaggcgtt gttttacaat
    61981 gcctggagtc gcacgaacag ttattttcag aataccgaat acgcggccag aaattacccc
    62041 gacgccgcgg agctggtgct tctggcacca atgaaaaaag atctaccgcc agaagtcttc
    62101 acacaaatct accagccgcc ggtatccaaa ggcgatggct acgatcgtga caacctgtta
    62161 aaagccgaca aacttctcaa cgaagcgggc tgggtgctga agggtcagca acgcgttaat
    62221 gccacaacgg gtcagccact cagctttgaa ttattgcttc ccgcaagcag caatagtcag
    62281 tgggtattgc cgttccagca cagcctgcaa cggctgggta tcaacatgga cattcgcaag
    62341 gtggataact cgcaaatcac taaccgcatg cgcagtcgcg actatgacat gatgccgcgc
    62401 gtatggcggg cgatgccgtg gcccagttcc gatttacaga tttcctggtc atcggaatat
    62461 atcaattcca cttataatgc ccccggcgtg caaagcccgg ttatcgactc gctgatcaac
    62521 caaattattg ccgcgcaggg aaataaagaa aaattactgc cgttggggcg agcactggat
    62581 cgcgtattaa cgtggaatta ttacatgctg ccaatgtggt acatggcgga agaccgtctc
    62641 gcctggtggg ataaattctc ccagccggcc gtgcgcccca tctatagcct cggtatcgat
    62701 acctggtggt atgacgtcaa taaagcggcc aaactgccgt ccgccagcaa acagggagag
    62761 tagatgggcg cttacctgat tcgccgtctg ttgctggtga tcccaacatt atgggcgatt
    62821 atcaccatca actttttcat cgtgcaaatt gcgcctggcg gtccggtcga ccaggccatc
    62881 gccgccattg agtttggtaa tgccggagta ttacccggcg caggcggtga aggtgttcgt
    62941 gccagccatg cgcaaacggg tgtcggcaat atcagcgaca gtaagtaccg tggcggacgc
    63001 ggattagatc cagaagtgat cgctgagatc actcatcgct acggttttga taagccgatc
    63061 cacgaacgtt acttcaaaat gctctgggac tacatccgct ttgattttgg cgatagcctg
    63121 tttcgcagcg cctcggtgct gacgctgatt aaagacagtc tgccggtttc catcaccctc
    63181 ggattgtgga gcacgctgat tatctatctg gtgtcgattc cgttaggcat tcgcaaagct
    63241 gtttataatg ggagccgctt tgacgtctgg agtagcgcat ttatcatcat cggctacgcc
    63301 attccggcct ttttgtttgc catcctgctg attgtcttct tcgcgggcgg cagctatttc
    63361 gacctgttcc ctctacgcgg cctggtttcc gctaactttg attcgctgcc gtggtatcag
    63421 aaaatcaccg attatctgtg gcatatcacg ctgccggtgc tggcgacagt gattggtggc
    63481 tttgcggcgc tgaccatgct gacaaaaaac tcattccttg atgaagtgcg caagcaatac
    63541 gtggtgaccg cgcgtgcgaa aggggtaagt gaaaaaaata ttctctggaa acatgtgttc
    63601 cgcaacgcca tgctgctggt gattgccggt tttccggcga cgtttatcag catgtttttt
    63661 accggctcgc tgctgattga ggtgatgttt tcactcaatg gtctgggctt actgggctac
    63721 gaagcgaccg tctcgcgcga ttatcctgta atgtttggta ccttgtatat tttcaccctg
    63781 attggcctgc tgctgaatat tgtcagtgat atcagctata cgctggtcga tccgcgtata
    63841 gattttgagg gacgttaatg tcgcgactca gccccgtcaa tcaggcccgt tgggcgcgtt
    63901 ttcgccataa ccgtcgcggc tactggtcgt tatggatttt cctcgttttg tttggtttga
    63961 gtttgtgttc tgaacttatc gccaacgata aaccgttgct ggtacgttat gacggcagtt
    64021 ggtatttccc gttgttgaaa aactacagcg aaagcgattt tggcggcccg ctggcaagtc
    64081 aggctgatta tcaggacccg tggctgaaac aacggctgga aaataacggc tgggtactgt
    64141 gggcaccgat tcgctttggt gctaccagta tcaactttgc taccgataag cccttccctt
    64201 ctccaccctc ccggcaaaac tggctgggaa cggatgccaa cggcggcgat gtgctggcac
    64261 gtattctcta tggcacgcgg atctcggttc tgtttggcct gatgctgact ctctgttcca
    64321 gcgtgatggg cgtgctggcg ggggcgctac aaggctatta cggcggtaaa gtcgatctct
    64381 gggggcaacg ctttattgaa gtatggtcgg ggatgccgac gctgtttttg attattttac
    64441 tttccagcgt cgtacagcct aacttctggt ggctgctggc aattactgtc ttgtttggct
    64501 ggatgagtct ggtcggcgtg gtgcgggcgg agtttttacg cacccgtaat ttcgactaca
    64561 ttcgtgcggc gcaggcgctt ggcgtcagcg atcgcagtat catcctgcgt catatgttgc
    64621 ctaatgccat ggtcgcgacc ctcacctttt taccgtttat tttatgtagt tcgataacca
    64681 ccctgacctc actcgatttc ctcggcttcg gtctgccgct cggttcaccg tcactcggtg
    64741 aactgctgtt acaagggaaa aataaccttc aggctccgtg gcttgggatc accgccttct
    64801 tgtcggtggc gatattattg tctttgctga tctttattgg tgaagccgtc cgcgacgcat
    64861 ttgatcctaa taaggcggtg tagcatgacg caaactctgt tagcgattga agatttgtcg
    64921 gtgggttttc gccatcagca aaccgtacgt acagtagtca atgatgtttc actacagatt
    64981 gaggctggcg aaacgctggc gctggtgggt gagtcaggtt caggcaaaag cgttaccgcg
    65041 ctgtcaattt tacgcctgct cccttccccg ccggttgaat atctctccgg cgatattcgt
    65101 tttcgtggcg aatcgctgct tcatgccagc gatcaaacgt tgcgcggtgt acgcggtaat
    65161 aagatcgcca tgatttttca agagccgatg gtgtcgttaa atccattgca taccctggaa
    65221 aaacagcttt atgaagtgct ttcactccac cgcgggatgc gtcgggaagc ggctcgaggc
    65281 gaaattctta actgccttga tcgcgttggt atccgccagg cggcaaaacg gctgacagat
    65341 tatccgcatc agctctccgg cggcgaacgc cagcgggtga tgattgcgat ggcgctgtta
    65401 acgcgaccgg aattattaat tgccgatgaa ccgaccaccg cactggacgt ctctgtccag
    65461 gcgcagattt tacagctgtt gcgcgaactg caaggcgagc tgaatatggg catgctgttt
    65521 attactcata acctcagcat tgtcagaaaa ctggcccacc gcgtggcggt aatgcaaaac
    65581 ggtcgctgtg tcgagcaaaa taacgccgct acgctatttg cctcacccac tcatccttac
    65641 acacaaaagc tactcaacag tgaaccgtca ggcgacccgg tgccgttgcc agaacctgcc
    65701 tcaacgttgc tggatgttga acagcttcag gttgccttcc ccattcgcaa agggattttg
    65761 aagcgcattg tggatcataa tgtggtggtg aaaaacatca gttttacgct acgagcgggt
    65821 gaaacactgg gtttagtggg cgagtccggt tccgggaaaa gtacgacggg actggcgctg
    65881 ctgcgactga ttaattctca gggcagcatc atctttgacg gtcagccact gcaaaattta
    65941 aatcgccgcc agctgttacc tattcgtcat cgcattcagg tggtatttca ggatccaaac
    66001 tcctcgctca acccacgact caacgttttg cagattattg aggaaggctt acgggttcac
    66061 cagccgacgc tttctgccgc acaacgcgaa caacaagtga tagccgtgat gcatgaagtg
    66121 ggattagatc ctgaaacacg ccaccgttat ccggcggagt tctctggtgg tcagcgacaa
    66181 cgtattgcga ttgccagggc attaattctt aagccctcgc tgatcatact tgatgaaccg
    66241 acatcatcac tcgacaaaac ggtacaggcg caaatattga cgctattgaa atcattgcaa
    66301 caaaagcatc aactggccta tttgtttatc agccacgatt tgcacgttgt ccgcgcgtta
    66361 tgtcatcagg ttatcgtact gcgacaaggg gaagtagtgg aacaaggacc gtgcgcgcgc
    66421 gtgtttgcca caccgcagca ggagtatacg cgtcagctac tggcgttgag ctgacgctta
    66481 aaaaggattg tagtttgaaa agggttcggc aattgccaca ccaaaatttt tcaatcggca
    66541 tgttgcagca aactcgtcct ggcgatttac aaacaggcac ggctcacctt cacactccag
    66601 caccgaacat ggaacttcga tgtcgcttaa cgcctggctt agtttataca tcaccgacca
    66661 tgcattatca ccatccgggc taagcaattt aagagccacc aggctgttgt caccgcacgg
    66721 tccattttgc ggaatcggtt caaccttcga acctgcgaaa ccagcagacc agcgatagtt
    66781 ctgcggcagt cgatggacga ttgagagttg taatgaagtc acttattttc cccggaagca
    66841 cattcacttc acaatttgtt tacatcaatt ttaacacatc atcaacaatc cgtctttata
    66901 cagatcgtaa agattgatgc ctcgcctgcg tggcctctat ggctctgatt taagtaatat
    66961 ctgcgggcgt ggtcacgttt gcggggctat atgtacccgt ttctgcgatc taactcaacc
    67021 tttttaacta caatgatgtg actttttaca taaattgatt ttacataaaa taaacatata
    67081 tcgggggaat gatagatttg tggttgacat gcatcaacaa aggaagcctt ttagcttcct
    67141 cgttgtgcaa tagatcaccg ttttttcggt cgactggcgt acagacagaa gagaatggag
    67201 ctgcttgcgc agaatgcaat tgaccaaatc atcggccatg cagagttaaa ggtcgcaaga
    67261 gaaagcaatg cgccaacaat tgcccctatg ccaaaacgga aggttcctgc cagcgaagat
    67321 gccgttcccg ccatatgggg aaactcatca agaatgaccg ccatcgcatt ggatgacacc
    67381 atcgacacgc agcccacaaa cgccgcaacg ccaaccacca gcgaccagaa tcccagcccc
    67441 agcagcgcac tgatgaccat ccacgctgcc ataataaatt gtatccacaa ccccgagcgg
    67501 aacatattta acgcgccaat gcggcgaacg aagcggctgt taaaaatggt catcacgaac
    67561 aggaaaacaa tgtttagtgc aaagtaataa ccaaagtttt ccggcgcgac gtggttaatt
    67621 tcaatgtaga caaacggtcc ggcgcttaag aacgagaaca tcccggcaaa gctgaaacca
    67681 ctggcaagca tataactcag gacacgctta tgacggaaca gcgccgcaaa gttaccaata
    67741 gtggtacgaa tgtgaaaagg ctggcgacgc tccggtggta aggtttcttt aatcaggaag
    67801 aaaatcattg ccgaagccag aatcgccgct aatgccagga tccagaagat gtaatgccag
    67861 ctcagccaca ccagcaccca accgccgaca atcggtgcca tcagcggtgc aatagtggtc
    67921 accagcatga caaacgacat catccgcgag aactcttctt tcgggtaaat atcgcgcatc
    67981 agggcgttaa tgaccacgct ggccgcagcc gccgccagcc cgtggaagaa acgcatcaca
    68041 atcagctgat cgatggtgtt tgccaacgca cacgccaccg cggcggcggc aaacaccagc
    68101 gtaccgccga gcaccaccgg cttacgcccg aagctgtctg ccatcggccc gtagattaac
    68161 tgccccaacg caaagcccag aatataagta ctgagggtca tctgcgtact gcccgcaggt
    68221 acgccaaact gcgctgaaat taccggtagc gcgggcagat acatatcaat cgacagcggc
    68281 atcaacatgg ccagcaggcc aaggataaaa acaatagcaa acgacgaatg ctgtcgggtg
    68341 gtcacaacgg gctcctgaaa gtcatggaaa agttagacga cgctggcaat ttcttcttca
    68401 gttaacggac gatattcacc gggggctaaa tcagcatcca gcgtaatacc gccaatacgt
    68461 tcacgatgca gctcaaccac gtggttaccc acggcggcga acatgcgttt cacctgatga
    68521 taacgccctt cgctgatggt cagacgaacc tgcgttgggg taatcacttc cagcaccgca
    68581 ggcttagtga gatctttttc gttatgcagc tgcacgcctt tagcaaattg ctctgccgta
    68641 tcgtcagcta caggtgattc cagtgtcacc agataggtct tctcgcaatg atggcgcgga
    68701 gaagtaatgc ggtgcgacca ctgaccatca tcagtcatca gcaccagacc ggtggtatcg
    68761 atatccagac gtcctgcggc gtgcagtttc caggctaccg gttcatccag aaaatagagc
    68821 accgttgggt gatcagggtc gtccgtggag caaacatagc cctgaggctt attgagcatg
    68881 aagtaacgtg gaccatgttg ctgcgccagc gggttgccat cgtaagcgac atcatgttca
    68941 ggaagcagtt tgaacgctgc attacgaaca atttcgccat cgacggtgac acgattgccg
    69001 cggatttcac gcccggcaat agcacggcta acgccgagtt gctgtgcgat aaatttatca
    69061 agtcgcatgt gtgtgatttt gcctgtaaaa acggaggtcg ggcattgacc cgaaaatctg
    69121 aactgttgtc tgcccagtat agcggtctaa taacatccct caagggaaaa agatccatgg
    69181 catactatta gcagaataat ctacctacgc gagaccatga tttttacact ccgcccatat
    69241 cagcaagaag ccgtggatgc cacgctcaac cattttcgtc gtcataaaac ccctgccgtt
    69301 atcgtgctgc ccaccggcgc aggtaaaagc ctggtgattg ccgaactggc acggctggcg
    69361 cgtggtcgtg ttctggtgct ggcacacgtc aaagaactgg tggcgcaaaa ccatgcaaag
    69421 tatcaggcgc tggggctgga agccgatatt tttgccgctg ggttaaagcg caaagagagc
    69481 cacggtaaag tggtatttgg cagcgtgcag tcggtcgctc gtaatcttga tgcctttcag
    69541 ggtgaatttt cgctgttgat tgtcgatgaa tgccaccgta ttggtgacga tgaagagagc
    69601 cagtatcagc aaatcctcac tcacctgact aaagtgaatc cccacttacg cctgctgggg
    69661 ctgactgcca cgccttttcg attgggcaaa ggctggatct accagttcca ttatcacggc
    69721 atggtacgcg gcaatgagaa agcccttttc cgtgactgca tttatgagct gccgctgcgt
    69781 tatatgatta aacacggcta tctgacgccg ccagaacgac tggatatgcc agtagtgcaa
    69841 tacgatttca gccgcttgca ggcacagagt aacgggctgt tcagcgaagc tgatctcaac
    69901 cgtgagctga aaaaacagca acgcattacg ccacatatca tcagccagat catggagttc
    69961 gccgaaaagc gcaaaggggt aatgattttc gccgccaccg ttgaacacgc aaaagagatt
    70021 gtgggattac tgcctgccga agatgctgca ctgattactg gcgacacccc cggcgctgag
    70081 cgcgatgtgt taattgaaga ttttaaagcc cagcgttttc gctatctggt caacgtcgca
    70141 gtactgacca ccggatttga cgccccgcac gtcgatctta tcgccattct gcgccctacc
    70201 gaatcagtga gtctttacca acaaattgtc gggcgcggtc tgcgtctcgc tccgggcaag
    70261 actgattgtt taattcttga ttatgcgggt aatcctcacg atctctacgc gccggaagtt
    70321 ggtacaccga aaggcaaaag tgacaacgtt ccggtacagg ttttctgccc tgcctgcggt
    70381 tttgccaaca ccttttgggg gaaaacgacc gccgacggga cattgattga acactttggt
    70441 cgccgctgtc agggatggtt tgaagatgac gacggtcatc gcgaacagtg tgacttccgt
    70501 ttccgtttta aaaattgccc gcaatgtaac gcagaaaatg atattgccgc tcgccgctgc
    70561 cgcgaatgtg acacggtact ggttgatccg gatgatatgt taaaagcggc gctacgactg
    70621 aaagatgcgc tggtattacg ctgtagcggc atgtctttgc agcatgggca cgacgagaaa
    70681 ggcgaatggt tgaaaatcac ctattacgat gaagacggcg cggatgtgag tgagcgtttc
    70741 cgtctgcaaa cgcccgccca gcgaactgcc ttcgagcagc tttttatccg cccgcatacg
    70801 cgcacaccgg gcatccctct gcgctggatc accgccgccg atatcctcgc ccagcaagcc
    70861 ttactgcgac atccggattt tgtcgtcgcc cgcatgaaag gtcagtactg gcaggtgcgt
    70921 gaaaaagtgt tcgattacga aggtcgtttt cgtcgggcgc acgaattacg cggttaatgg
    70981 tagttttgat tgatgtataa ggcgattgag tatagaatct cgcccgcttt tgcatacgca
    71041 aagcagatca cttacctgtt gctgggtcgc ctgtagcagg attaatttaa gagagaaaga
    71101 aatgtttact atcaacgcag aagtacgtaa agagcagggt aagggtgcga gccgccgcct
    71161 gcgtgccgct aacaagttcc cggcaatcat ctacggtggc aaagaagcac cgctggctat
    71221 cgagctggat cacgacaaag tcatgaacat gcaagctaaa gctgaattct acagcgaagt
    71281 tctgaccatc gttgttgacg gtaaagaaat caaagttaaa gctcaggacg tacagcgtca
    71341 cccgtacaaa ccgaagctgc agcacatcga cttcgttcgc gcttaattgc tgaataagtt
    71401 gtaaaaaacc ccgctccggc ggggtttttt gtatctgcag attatgcctg attacggtat
    71461 tgctattttt tgcaggccag ataaggcgtt cacgccgcat ctggcattca gaacacaaaa
    71521 ccgattaatt gccgccagac gtccggcgct gcaattggtc gcgcaaattc ggcggcgtgc
    71581 ctttaatggt caaggtatcg gttgctggat cccagaagat gcgttcacct aacagcatcg
    71641 catcaaagtt aatagtcaag ccaccgccgc taccggcaaa cttcgtcaac tgacgcagtg
    71701 tgctacggtc tgccgggaag ctctcttcca gttcgtagcc tttttcagcc gcaaactccg
    71761 tgaagctcac ttcgcttacg cctgccagct ctttcgacag cgattccagt tcgatctctt
    71821 cgcctgcctg cagctgctca ttacagtagc tataaacctg ctggcgcacg ttctgtcgct
    71881 ctgctttatc cagttgtgct tcggcagtga aatcatcaac cgcttgcaac agcccacggt
    71941 tttgcgcttt cgcgttcaat ccttcgctgg ccccgaggaa atccataaag aaatccgcca
    72001 ctttgcgccc tactcgccct ttcaggaaag tgagatagcg ggtggactct ggattggttt
    72061 cccattcggt taaatcgata cgcgcaacaa tatccgcatg attgatatca agataatggg
    72121 ttgggttgat gtcgagattt tcgttaacgc gcatactgct caggttgctc agaactgcca
    72181 ccagcagata ctccaccgcc agataacggt agtggcaaaa cagcacgaat ccaccgtcag
    72241 caaaaggata tttcgctaat tcgtcacgca ggcgcccggt tgccgcgcgg ctaaatgcca
    72301 gaaagtcctc ttcgccctga cgctgtaagc gcagcgtctg cgccagttca ctctcttcgc
    72361 taaacaagcc gtaagcttta tttttggcgc tatagacccg atgcagttct gccaccatct
    72421 caacgacggt ttctgtcggt tccagcaatg aatcgcgcaa caccagctca aggttctgct
    72481 catcacgctt gataagctgg tgcagggcaa tctggttgat atccagactc atgataaact
    72541 ctcctttaag accgggcggt attcaaccac cgccggttaa gcgacgcaat aaaagatggc
    72601 ggtcatttta cagaaggcag cgtaacgaga atgatttaac gcataaaaaa gcagaaaaaa
    72661 aaccgttgct acggtaatat gttgcccttt catcaactaa ctgatttcga tttatgccac
    72721 aaatttcccg ctacagtgat gaacaggttg aacaattgct cgcagagctg ctcaacgtac
    72781 tggaaaaaca taaggctccg accgaccttt ccctgatggt gttaggcaac atggtcacta
    72841 accttatcaa caccagcatt gccccggccc aacgccaggc aattgccaac tcttttgccc
    72901 gcgccttaca gtcctctatc aacgaagaca aagcgcacta agggaaacag ataactggtt
    72961 atggtaactc atcgtcagcg ctaccgtgaa aaagtctccc agatggtcag ttgggggcac
    73021 tggtttgcac tgttcaatat tctgctttcg ctcgtcattg gcagccgtta cctgtttatc
    73081 gccgactggc cgacaacgct tgctggtcgc atttattcct acgtaagcat tatcggccat
    73141 ttcagcttcc tggtgttcgc cacctacttg ctgatcctct tcccgctgac ctttatcgtc
    73201 ggctcccaga ggctgatgag gtttttgtcc gtcattctgg caacggcggg aatgacgcta
    73261 ttactgatcg atagcgaagt ctttactcgt ttccatctcc atcttaatcc catcgtctgg
    73321 caactggtta tcaacccaga cgaaaatgag atggcgcgcg actggcagct gatgttcatc
    73381 agcgtgccgg ttattttatt gcttgaactg gtgtttgcga cgtggagctg gcaaaagctg
    73441 cgcagcctga cgcgtcgtcg acgcttcgcg cgcccgctgg ccgcattctt atttatcgcc
    73501 tttatcgcct cgcatgtggt gtatatctgg gccgatgcca acttctatcg cccgatcacc
    73561 atgcagcgcg ctaacctgcc gctttcgtac ccgatgacgg cgcgacgttt tcttgagaag
    73621 catggtctgc ttgatgcgca ggagtatcaa cgccgtctta ttgagcaagg taatccagac
    73681 gccgtttccg tacagtatcc gttaagcgaa ctgcgctatc gcgatatggg caccgggcag
    73741 aatgtgctgt tgattactgt cgatggcctg aactactcac gcttcgagaa gcagatgcct
    73801 gcgctggcag gttttgctga gcaaaatatt tcgttcacgc gccatatgag ctccggcaac
    73861 actacagaca acggcatctt tggcctgttc tatggcatct cgccgagcta tatggacggc
    73921 attctgtcga cccgtacgcc tgcggcatta attactgcgc ttaatcagca aggctatcag
    73981 ctggggttat tctcgtcaga tggctttacc agcccgctgt atcgccaggc attgttgtca
    74041 gatttctcga tgccgagcgt acgcacccaa tccgacgagc agaccgccac gcagtggatc
    74101 aactggctgg gccgctacgc acaagaagat aaccgctggt tctcgtgggt ctctttcaat
    74161 ggcactaaca ttgacgacag caatcagcag gcatttgcac ggaaatatag ccgggcggca
    74221 ggcaatgtcg atgaccagat caaccgcgtg ctcaatgcac tgcgtgattc tggcaaactg
    74281 gacaatacgg ttgtgattat cactgccggt cggggtattc cgctgagcga agacgaagaa
    74341 acctttgact ggtcccacgg tcatctgcag gtgccattag tgattcactg gccaggcacg
    74401 ccggcgcagc gtattaatgc gctgactgat cataccgatc tgatgacgac gctgatgcaa
    74461 cgcctgctac atgtcagcac acctgccagc gaatattcgc aaggtcagga tttgttcaac
    74521 cctcaacgcc gtcattactg ggttactgca gcggataacg atacgctggc aattaccacc
    74581 ccgaaaaaga cgctggtgct gaacaataac ggtaaatacc gcacttacaa cttacgtggt
    74641 gaaagagtga aagatgaaaa accacagtta agtttgttat tgcaagtgct gacagacgag
    74701 aagcgtttta tcgctaactg attaattata aatcagttag cgaaatatct tacttgcaat
    74761 cggtgtggaa aacggtagta ttagcagcca cgagtcggca cgtagcgcag cctggtagcg
    74821 caccgtcatg gggtgtcggg ggtcggaggt tcaaatcctc tcgtgccgac caaatcgccc
    74881 accggatatc accggaaagc accggataag acagggataa cccgctaatc acttgattgg
    74941 cgggtttttt tatgtttttt agtcaccgaa catcaccgga tttataagct atcagccgga
    75001 ttttttagtt atacgtttag ttatacgggc tggcgtataa ctaaagccgt ataactaaaa
    75061 ccctgttttt tcgtgtggaa tcatagacat atggcgagaa aaacacaccc attaaccaca
    75121 gtgcagatca aagcagccag accagcgcaa aaggagtaca ccctgcagga cggcggaggg
    75181 ctttttctcc tggtcaaacc gtctggatca aaactctgga gattttccta ctaccgacca
    75241 tcggacaaaa aaagaatatt gctgagtttt ggatcgcttg aagatgtttc cctggctgat
    75301 gccagaaaac gccgtagcga gtacaggacg ttaatcagtg ccggaactga cccgcaggac
    75361 cacgagaggc aaaaaagaga gacagaggcc cgaagacaag ggaacacgtt cgaaaatgtg
    75421 gcggcggcat ggtaccaggt gaaaatcagc cagaatctgg cccccaacac gattaaagac
    75481 atctggcgtt cgctggataa atatgtattc ccgttcatcg gcaacacgcc aatagatacc
    75541 ctcaccgccc gaaggttcgt tgaagtgctt acccccatca aggagcgcgg caacctggaa
    75601 acactcaagc gggttttaca gcgcgttaat gaggtaatgg attacgccgc caacagtggg
    75661 ctgattgatg ccaatccggc tatgaatgtg cgtaaggcgt ttccctcacc tgtaaaaaaa
    75721 catatgccaa caatccgccc cgaacagctg cccgagctta tgcaggcttt atcagtatcg
    75781 gcaacagaac ggcagaccag attactgatt gaatggcagt tactgaccgt aacccgtccc
    75841 gccgaagcgt catcaacgcg gtgggatgaa atcaacctgg acgcgaagca atggacgata
    75901 cctgccggac gcatgaagat gcgcagggat cacgttatcc cgctttccgg tcaggctatg
    75961 gcggtgctgg aggccatgaa accaatcagc caccaccgca attacgtttt cccaagtctg
    76021 aaagacccac agcagccgat gaacagccag acagctaacg cagcattgcg gcgtatggga
    76081 ttcgctggcg tgctggtgtc tcatggatta cgcgccatat tcagcacagc agcgaacgag
    76141 gaaggattcg agccggacgt aatagaggcg gcacttgccc acgtcgacac caacgaagtt
    76201 agacgggcat acaaccggag caactacata gaaaaacgca tcgtgctgat gcgctggtgg
    76261 ggcgaatttg tcgaggctgc ggcgacgggc gtaaccctcg ccagtggtaa aaggggtatc
    76321 cgagccgtgt agctgtacag aaaaccagta aaaactacga aaaccatgta aaaccgtcgt
    76381 ataattgcat caaatttaac gacaaggccg tgaaacatga aaccgttaag atgcaaaaaa
    76441 atatcagatg caattgcgac gggctgcaac tggccctgat ggttcagcat gaattttggt
    76501 caacctacga tccggaggac agaacgacgg ccccatcaaa aaaacaggta gtagattttc
    76561 tggtatcccg tggcgcttcc agaaatctgg cggtaagtat tgataaggtc gtacgtccgg
    76621 catctatgaa gatcggaggc aggcccaaaa aatggcggta acaatcctgg aagcggcaga
    76681 aatgccgctt tttttataat tccatttcaa atcatcaata taaaaaacgg tgtataccgt
    76741 ttaaaaacgg tgggaactgt ttttacccat atccgatgat ttaccgtatt tgtcaccgga
    76801 atacaccgga ttcacaaggt aaatcacgat ggaagcaatc agaaaaatta tctttcgcca
    76861 ggaagtaaaa aaaattatcc acatcaaggc agacagcacg ctgcaaagca tgatcaacgc
    76921 cggagaattt ccgcagggtt ttcgcgttgg tttacgccgt cgcggatggt atgaggatga
    76981 tgtgttgtcc tggcagaaag aacgcgaaga ggaagcacgc ggaacggctg cttaacgggt
    77041 atcacagata tgacaaacac gaaaaaagcg gccccgatat ggagccgcct ttctgaacaa
    77101 ttaacccgct gcgccttatg tgtatgtgat cccaaacata agcacgggga tgatagccgc
    77161 tatcaggctg gtgggcaatg caatcagtct ggttcagttc gttgccatac ctgcaatgag
    77221 cgcttttccc tgtactcttt aaggaattgc tcaagggcaa aagcacatgg cgcgaatctt
    77281 tctgattcat gctctatctt tctgcgccgt cttttccgtg ccggtgataa tgttttggtc
    77341 aattctttat cggtcattgt gttgtcctgc atagcaatgc gccgtaatac ctcacaccac
    77401 ggcgctgata gtttttatcc tttgggttct atgccgcgtt gctgtaattc tttgcggaga
    77461 acgcgtttaa tccaagtggc taaagatgtg tcgccatcgg ccttagctgc ttcttctagc
    77521 tgcgctctga agtcttccgg taatcgcatt tggtatggag gcgaacgttt ttctaatggt
    77581 gttgacacgg taattaccct tcattactat acctacatgg taattaccat tgtacttgcc
    77641 attacacaaa aatcaacgcc ccgcagtgct gggaacacat acggggcgtc taaccaacaa
    77701 cgtaaactag gagccgttat ggttgccgta aatcatatac cacaccttgt acacacacaa
    77761 acggcctttg tgtggcgttt tctggccctg aatatcggag aaaaaaacca gttgatcgcc
    77821 agtgtcgaaa atagctatga ttcccgcgca cctcataaaa cgggtgccgg gattggcgtc
    77881 ctgggcaaaa ctgaagcgca taacacgcgc cccgcgtgtt tttttgtgcc gcatagtcac
    77941 accttatcaa tggtgggctg tacgggggcg gagcaatccg cgccggtttc ttcagtgtcc
    78001 ggttacgcca accctgtaca gtccgccacc agcgaaattg gcgtttccgg tggcggttat
    78061 cttaaccaca ctgaggaggc tgccaacatg gctactatcc ctacccttgt acattctcaa
    78121 accgcctttc tctggcgctt tatcatcttt ggcgcgtcag aacatcaaat catccacgta
    78181 accgcctgga cggaacgcga agcgcgtagc cgttgcccgt caggttgtgt tgctgtattt
    78241 gccgctcgta ttcgtcaggg gtcacatcat gcgtaaaaac cgcttgcaaa aaattatcac
    78301 ggggctgtat gcttcacccg tcgcccacat ggcgaccggg tttagcagcc tgaatactgt
    78361 agcggacagc cgcttacatt ccgatatgcg gtttttttgt gtccgtaaac ctacccatac
    78421 ccgcattatg gcggggcgta acgggggagc ctttgcgctc gctggttcct acagtaccag
    78481 tctgctaacc ctgttacgtc tcgccacccc gtttagcagc gtagtagcga gactccttaa
    78541 aattactgta ggagcctttc acatggctgt atccgcacgc ccttactttg tctggcgctt
    78601 tatgcagtgc caccacagca acattgatgt attcaccgtt accgcagcca ctgaacgcga
    78661 agcacgcgcc cagttgccgc acgcacatct gatttttgtc gcccgtattc gtcagggggt
    78721 gagtcatgca taaaataccc tatgatgttc ttattcattc tgaaaacgca ttaattaaag
    78781 cgcgtgaaat gaatgcgctg ttaattaagt taatagacgc gcaggaagat ggtggagaac
    78841 ctgcatcatt aatatttgcc gccgttcaga cattactatc gcctgtaatt gatgaactgg
    78901 ataaggcgat ggcaattcac gaaaataata aagcgcccca caccggagaa taaaaataat
    78961 gaaacttaaa tattctggct taactgccag cggcaaaact cgcactaaat tcatgtgcgg
    79021 ggatatttac cgcgataagt acggcggcac ggtaatgatt aagggcgtgg atgaacggcg
    79081 cgtaacttac cgccgtgaag gctacgaata tgattgcgtt atgcctgttt atcagttcga
    79141 ccgtgatttt tatctggtac aggcagcgcc ccgcagtaag cccaccagca gtgagaaagc
    79201 acgcgccaat attcaggaaa taaaaaagat gttgaacgta ttcaggggta aaaaatgaaa
    79261 cttgcaccga acgtaaaacg attaccgaaa gacaaataca ccgaagcaat tattttcgcg
    79321 gggaatgatg cgcactcatt tgcagagcac tacatcattg cacaggccaa aaaagcaggc
    79381 gatccagtcc cccccgttta tctggggtgt tatcagttaa gcgaactgga taacctccag
    79441 attgttgatg atgggcgata cagggcgacg gtgatacgcg ccggaaatat tgaagagccg
    79501 cagctgttaa ccatcgccac gaaactggcg atcgccggag tccaggaggc gcggctactt
    79561 tctgaaaatt tcgaattgct ggaggaatgg agcgaccagc ttccacggct tagggaggca
    79621 tgggaacgcg gggaaagcct ggtcatgaaa aaaatccccc agcggaaaac cacgctaccg
    79681 atgagcgttg gttcttccgg atacgacacg cagcttgatt acgtggtaaa ggggattatc
    79741 ccggcagtat cgctgtgcag catatacggg gcgagcggtt catataaatc gttccttgcg
    79801 ggttcgtggg cgtgtcacgt ttccacaggc cgccagtggg gaggccgcag ggtggcgcat
    79861 ggcgctgttc tctatgtggt tggtgaaggc ggtattggcg ttccgcgtcg tgtaaaagcc
    79921 tgggaggttg tgcacgatga gcaggtgaaa aatctgtatc tggtaaaccg tcccatcttt
    79981 ccggctgccc cgcttgatgt tgaggaaatg gttatcgctg cccgtcaggt ggaacgggaa
    80041 acgggtaaac cggttcgaat gattattctg gatacgctgg cgcgctgctt tggcggcaat
    80101 gatgaaaatg attcccgtga tatgggggcg tttatccgtg ggtgtgacga actgaaacgg
    80161 cgcacagggg caacggtgct ggtggttcac cattccggca aggatgaaac aaagggggcg
    80221 cgtggttcca gtgcatttcg tgcatcactg gacgccgaat accggatacg cagggaggac
    80281 gcaggcagcg aagcgctggt tatctcatgt accaaaatga aggacgcgga ggaactcaaa
    80341 gaagcagcat atgacttacg cgtggtggag ctttttaccg acgctgacgg ggagttaatc
    80401 acgtcgctgg tggtggtgga taagccgcgc cctcccgttg aactggagcg catcgaggag
    80461 gcagggaaca agacggagaa tcacgccgcg ctatggcagt gcatcagaac acgaacagca
    80521 cacggggagg cgtgcactat cgcccttctc cgcgacgata tgaaaaagct ggggtatgag
    80581 atgaagcact tccggcgctg gctgtacaag ctggaaaatg atggcgtaat cgttattgac
    80641 ggtgatgacg tgcgcccgct gtaaaaagtg gggagtaaaa gtggggggag tggggaattt
    80701 aacaaaattg aaacataatt acccactttc ccacctgtat atacccaaaa aagtggggag
    80761 taaaaaacat attgaaaaac ataacgttag aatcacaaaa aaaagaggtg gggagacggt
    80821 gggtaatttc aaaaagtggg gagcaaaagt ggggagtagt ggggaatgaa cagaaaaacc
    80881 agagataaga cagcgccaaa atataaagcg ttagacatga cagagctttc cttaaaggtg
    80941 gcaatcagaa cgatagaccg ccacgcgggg gaaggatacg cgaaggagca tcccgaactg
    81001 ataagcgcat tcatgaccac agcggcggca aactttgcca cgctgacaga acgggagatt
    81061 gccgaagcgg aacaggtaac aaccatcaac gttaaaagcg ctggagaata accgaagaag
    81121 tgagcacata gccggagcaa tccggctttt tttcgatctc atatatcttc aaagatggtt
    81181 aattaatgat aatctatcga aaatactggt tatgcaatga taagtatcag gagaaaacaa
    81241 agtggaagag ataaagcaaa gcgctaccgt gatgattgat catgaaatcc ttaacgagct
    81301 taaaaagttg cagttcgata ttgtgcgccg gatgaatgaa tcaggtatct acggcagggc
    81361 ggcaacacca acaatagggc atcttgcgcg cgtactgtta cgcgaacgcc tgggcattgc
    81421 aacaagtgag gcgtaaacaa tggctatcac agaacgcgag gcaagcatca ttaaagccat
    81481 tggcgaggaa acaagagaca ccatagcgcc attgatggaa aagatcgcag agcttgagac
    81541 acgaataaac aagctgagtg atgaagttga aggcctgatc tcgttactcg gtgagcaatg
    81601 acaggggggg agtgttccgg ccggaaagtc gatcgccctg gacaccgccc ccagtctcat
    81661 tcatagaaaa ttttgctttt caaaaaagtt acacgaaaag ttacaccccc tcaaaacgag
    81721 gtttttgcat ggcattaacg accaaaaaaa agcggtttgc tgatgcgtta tcaaagggca
    81781 caaatccaac ggaagccgcg atacatgcgg gatacagcga aaagactgca agggttaaag
    81841 gctggcagct atccaatgat ccggaggtga aaaagtatct taaggctgta acttctgatg
    81901 taacttttca ggttacaaaa ccgaaaaaag ttacaaagaa agttaccgaa aaagttacac
    81961 cggaacgggc atcaattaac accattgaga tgatggataa cggcctaccc gaccccatca
    82021 aagcaatggg caggatactg attgacaaca tggacacaga tccaaaactg gctttagatg
    82081 ccgcgtttaa gctggcccag ttcacccacc gcaagatcgg cagcattggg aaaaaagagg
    82141 ttaaaaaact caatgccgct aatgtctcct caaatcggtt ccccatccct aagccaccaa
    82201 ctggcttaaa acactgaggt agcgaactat gagcgaatac atcacagacc ctattgatgt
    82261 actcacggaa atcatgaacg acaagcagca gccaaccgaa atacggttac aggctgccgc
    82321 tgttctgatg ccatattttc acgaaaccct aaccccatgt taccaggagg aagaagataa
    82381 tgatttctaa aaacatgaaa ggcgtgtcgg cagttcgcgc cgccagcgtg cttacatcgt
    82441 gtaaattcag ccatgcagac gcaattgaaa tgacgaaaaa aaatgatccg gcagtatcga
    82501 aatttcttga aaaatccatt gttgccggaa tgactacgga taatatgccg tcgctggtgg
    82561 cagagccgat agtaagagag cttggcgagt atattttttc gcagtctgta ccaggcaagc
    82621 taatcagcag cgcgctgaat cttccgtttg gcgcaaaatt gcagtcaata tcaggcaagg
    82681 gtagtaaatg gcttaaagaa gccgaagaaa tccctgtcaa agagggagaa gtaaacgaaa
    82741 gtgcgctaaa actgtgcaaa cttggcgcta ttgtgatgct gaataacgag ctaatcaaat
    82801 ttagcacccc tggtaccaat tcagcgatca gagacttgat cacatcggaa tcggtgaaag
    82861 aaatagataa aaaatttctt tccagtgacc aggaggtggc gggcgtatcc cctgagggcg
    82921 tgcttaatgg tgctgatgag gccgaaaact tcatagagct tttcaaaaag cacatagcga
    82981 acggtaacac gcttgccacg tcgtcgctaa tcttgcccgt agaaaacgcc ttacagctta
    83041 ctgatgctga gttctggcag atagagcttt tgagaatacc ggtaattgtg agcgaacagg
    83101 cggatcgcat gatgctgata gacgctcaaa agctgattat taacgttgaa gccacggtta
    83161 taaccccaac tgatgaggcg gtaataaaaa ccgaatcagg gacggttagc cttttccaga
    83221 atgacgctac agcgttcagg gcaatcacct attgcggatg gcaaaaactc gataaggccg
    83281 tcaccgttct ggcgaaaagc tgatttttcc ggcatcaatc ccgatatggt gatccccata
    83341 tcgggataat tacgaaattt tcgcagttta attaatggtc agattaatcg ctaacccgct
    83401 gatattttcg gaaacctcaa tttgaggaag tcggaagcgt cacccttcaa atcctgaatg
    83461 gtcataatga ccacgcagac catcaaggca accataacga acatcgttac ggttggagct
    83521 tttaccacag gaaaactcgc atttaaattg tacacttttt cagcaaatga ttttagttat
    83581 acgtttagtt
//
LOCUS       DAZGJW010000003        80208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZGJW010000003 DAZGJW010000000
VERSION     DAZGJW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.003"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            163..1347
                     /gene="setC"
                     /locus_tag="ACHKIK_000200"
     CDS             163..1347
                     /gene="setC"
                     /locus_tag="ACHKIK_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFO9081440.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLGDE
                     LKARPIMVDFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMICIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            1458..2381
                     /gene="yicL"
                     /locus_tag="ACHKIK_000201"
     CDS             1458..2381
                     /gene="yicL"
                     /locus_tag="ACHKIK_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO9081441.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(2385..3203)
                     /gene="nlpA"
                     /locus_tag="ACHKIK_000202"
     CDS             complement(2385..3203)
                     /gene="nlpA"
                     /locus_tag="ACHKIK_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO9081442.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            3425..3718
                     /gene="yicS"
                     /locus_tag="ACHKIK_000203"
     CDS             3425..3718
                     /gene="yicS"
                     /locus_tag="ACHKIK_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO9081443.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIHEAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(3759..4949)
                     /gene="nepI"
                     /locus_tag="ACHKIK_000204"
     CDS             complement(3759..4949)
                     /gene="nepI"
                     /locus_tag="ACHKIK_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO9081444.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(5160..5612)
                     /gene="yicN"
                     /locus_tag="ACHKIK_000205"
     CDS             complement(5160..5612)
                     /gene="yicN"
                     /locus_tag="ACHKIK_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO9081445.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(5665..6999)
                     /gene="adeQ"
                     /locus_tag="ACHKIK_000206"
                     /gene_synonym="yicO"
     CDS             complement(5665..6999)
                     /gene="adeQ"
                     /locus_tag="ACHKIK_000206"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO9081446.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            7174..8940
                     /gene="adeD"
                     /locus_tag="ACHKIK_000207"
     CDS             7174..8940
                     /gene="adeD"
                     /locus_tag="ACHKIK_000207"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO9081447.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(8985..10376)
                     /gene="uhpT"
                     /locus_tag="ACHKIK_000208"
     CDS             complement(8985..10376)
                     /gene="uhpT"
                     /locus_tag="ACHKIK_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO9081448.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(10632..11951)
                     /gene="uhpC"
                     /locus_tag="ACHKIK_000209"
     CDS             complement(10632..11951)
                     /gene="uhpC"
                     /locus_tag="ACHKIK_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO9081449.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGVSALLLLPFLNAQAPREA"
     gene            complement(11961..13463)
                     /gene="uhpB"
                     /locus_tag="ACHKIK_000210"
     CDS             complement(11961..13463)
                     /gene="uhpB"
                     /locus_tag="ACHKIK_000210"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO9081450.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(13463..14053)
                     /gene="uhpA"
                     /locus_tag="ACHKIK_000211"
     CDS             complement(13463..14053)
                     /gene="uhpA"
                     /locus_tag="ACHKIK_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO9081451.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(14128..14418)
                     /gene="ilvN"
                     /locus_tag="ACHKIK_000212"
     CDS             complement(14128..14418)
                     /gene="ilvN"
                     /locus_tag="ACHKIK_000212"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO9081452.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(14422..16110)
                     /gene="ilvB"
                     /locus_tag="ACHKIK_000213"
     CDS             complement(14422..16110)
                     /gene="ilvB"
                     /locus_tag="ACHKIK_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO9081453.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDVFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(16216..16314)
                     /gene="ivbL"
                     /locus_tag="ACHKIK_000214"
     CDS             complement(16216..16314)
                     /gene="ivbL"
                     /locus_tag="ACHKIK_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO9081454.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            16879..16968
                     /gene="tisB"
                     /locus_tag="ACHKIK_000215"
     CDS             16879..16968
                     /gene="tisB"
                     /locus_tag="ACHKIK_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO9081455.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(17231..17305)
                     /gene="ysdE"
                     /locus_tag="ACHKIK_000216"
     CDS             complement(17231..17305)
                     /gene="ysdE"
                     /locus_tag="ACHKIK_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO9081456.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            17387..18571
                     /gene="emrD"
                     /locus_tag="ACHKIK_000217"
     CDS             17387..18571
                     /gene="emrD"
                     /locus_tag="ACHKIK_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO9081457.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(18579..19076)
                     /gene="yidF"
                     /locus_tag="ACHKIK_000218"
     CDS             complement(18579..19076)
                     /gene="yidF"
                     /locus_tag="ACHKIK_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO9081458.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(19073..19435)
                     /gene="yidG"
                     /locus_tag="ACHKIK_000219"
     CDS             complement(19073..19435)
                     /gene="yidG"
                     /locus_tag="ACHKIK_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO9081459.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(19425..19772)
                     /gene="yidH"
                     /locus_tag="ACHKIK_000220"
     CDS             complement(19425..19772)
                     /gene="yidH"
                     /locus_tag="ACHKIK_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO9081460.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            19882..20331
                     /gene="yidI"
                     /locus_tag="ACHKIK_000221"
     CDS             19882..20331
                     /gene="yidI"
                     /locus_tag="ACHKIK_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO9081461.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(20378..21871)
                     /gene="yidJ"
                     /locus_tag="ACHKIK_000222"
     CDS             complement(20378..21871)
                     /gene="yidJ"
                     /locus_tag="ACHKIK_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO9081462.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDGLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(21868..23583)
                     /gene="yidK"
                     /locus_tag="ACHKIK_000223"
     CDS             complement(21868..23583)
                     /gene="yidK"
                     /locus_tag="ACHKIK_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO9081463.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            23750..24643
                     /gene="yidL"
                     /locus_tag="ACHKIK_000224"
     CDS             23750..24643
                     /gene="yidL"
                     /locus_tag="ACHKIK_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO9081464.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(24640..25454)
                     /locus_tag="ACHKIK_000225"
                     /pseudo
     CDS             complement(24640..25454)
                     /locus_tag="ACHKIK_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25454..27070)
                     /locus_tag="ACHKIK_000226"
     CDS             complement(25454..27070)
                     /locus_tag="ACHKIK_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO9081465.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            27366..28082
                     /gene="yidP"
                     /locus_tag="ACHKIK_000227"
     CDS             27366..28082
                     /gene="yidP"
                     /locus_tag="ACHKIK_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO9081466.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(28079..29740)
                     /gene="yidE"
                     /locus_tag="ACHKIK_000228"
     CDS             complement(28079..29740)
                     /gene="yidE"
                     /locus_tag="ACHKIK_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO9081467.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDMPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(29937..30365)
                     /gene="ibpB"
                     /locus_tag="ACHKIK_000229"
     CDS             complement(29937..30365)
                     /gene="ibpB"
                     /locus_tag="ACHKIK_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO9081468.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(30477..30890)
                     /gene="ibpA"
                     /locus_tag="ACHKIK_000230"
     CDS             complement(30477..30890)
                     /gene="ibpA"
                     /locus_tag="ACHKIK_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO9081469.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(30884..30956)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            31196..31528
                     /gene="yidQ"
                     /locus_tag="ACHKIK_000231"
     CDS             31196..31528
                     /gene="yidQ"
                     /locus_tag="ACHKIK_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO9081470.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(31530..32744)
                     /gene="yidR"
                     /locus_tag="ACHKIK_000232"
     CDS             complement(31530..32744)
                     /gene="yidR"
                     /locus_tag="ACHKIK_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO9081471.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYMPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAEVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            32845..33909
                     /gene="cbrA"
                     /locus_tag="ACHKIK_000233"
     CDS             32845..33909
                     /gene="cbrA"
                     /locus_tag="ACHKIK_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO9081472.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEIYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKRIMRSGMAHIPQLKD"
     gene            complement(33906..35243)
                     /gene="dgoT"
                     /locus_tag="ACHKIK_000234"
     CDS             complement(33906..35243)
                     /gene="dgoT"
                     /locus_tag="ACHKIK_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9081473.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(35318..36466)
                     /gene="dgoD"
                     /locus_tag="ACHKIK_000235"
     CDS             complement(35318..36466)
                     /gene="dgoD"
                     /locus_tag="ACHKIK_000235"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO9081474.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(36463..37080)
                     /gene="dgoA"
                     /locus_tag="ACHKIK_000236"
     CDS             complement(36463..37080)
                     /gene="dgoA"
                     /locus_tag="ACHKIK_000236"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO9081475.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(37064..37942)
                     /gene="dgoK"
                     /locus_tag="ACHKIK_000237"
     CDS             complement(37064..37942)
                     /gene="dgoK"
                     /locus_tag="ACHKIK_000237"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO9081476.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(37939..38628)
                     /gene="dgoR"
                     /locus_tag="ACHKIK_000238"
     CDS             complement(37939..38628)
                     /gene="dgoR"
                     /locus_tag="ACHKIK_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO9081477.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            38906..39562
                     /gene="yidX"
                     /locus_tag="ACHKIK_000239"
     CDS             38906..39562
                     /gene="yidX"
                     /locus_tag="ACHKIK_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO9081478.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYHWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(39608..40420)
                     /gene="yidA"
                     /locus_tag="ACHKIK_000240"
     CDS             complement(39608..40420)
                     /gene="yidA"
                     /locus_tag="ACHKIK_000240"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO9081479.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMELPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(40535..40933)
                     /gene="yidB"
                     /locus_tag="ACHKIK_000241"
     CDS             complement(40535..40933)
                     /gene="yidB"
                     /locus_tag="ACHKIK_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO9081480.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(41173..43587)
                     /gene="gyrB"
                     /locus_tag="ACHKIK_000242"
     CDS             complement(41173..43587)
                     /gene="gyrB"
                     /locus_tag="ACHKIK_000242"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO9081481.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(43616..44689)
                     /gene="recF"
                     /locus_tag="ACHKIK_000243"
     CDS             complement(43616..44689)
                     /gene="recF"
                     /locus_tag="ACHKIK_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO9081482.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(44689..45789)
                     /gene="dnaN"
                     /locus_tag="ACHKIK_000244"
     CDS             complement(44689..45789)
                     /gene="dnaN"
                     /locus_tag="ACHKIK_000244"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO9081483.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(45794..47197)
                     /gene="dnaA"
                     /locus_tag="ACHKIK_000245"
     CDS             complement(45794..47197)
                     /gene="dnaA"
                     /locus_tag="ACHKIK_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO9081484.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNIPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            47494..47574
                     /locus_tag="ACHKIK_000246"
     CDS             47494..47574
                     /locus_tag="ACHKIK_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081485.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            47804..47944
                     /gene="rpmH"
                     /locus_tag="ACHKIK_000247"
     CDS             47804..47944
                     /gene="rpmH"
                     /locus_tag="ACHKIK_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO9081486.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            47961..48320
                     /gene="rnpA"
                     /locus_tag="ACHKIK_000248"
     CDS             47961..48320
                     /gene="rnpA"
                     /locus_tag="ACHKIK_000248"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO9081487.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            48284..48541
                     /gene="yidD"
                     /locus_tag="ACHKIK_000249"
     CDS             48284..48541
                     /gene="yidD"
                     /locus_tag="ACHKIK_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO9081488.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            48544..50190
                     /gene="yidC"
                     /locus_tag="ACHKIK_000250"
     CDS             48544..50190
                     /gene="yidC"
                     /locus_tag="ACHKIK_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO9081489.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            50296..51660
                     /gene="mnmE"
                     /locus_tag="ACHKIK_000251"
     CDS             50296..51660
                     /gene="mnmE"
                     /locus_tag="ACHKIK_000251"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO9081490.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLYDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            51903..51977
                     /gene="tnaC"
                     /locus_tag="ACHKIK_000252"
     CDS             51903..51977
                     /gene="tnaC"
                     /locus_tag="ACHKIK_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO9081491.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            52198..53613
                     /gene="tnaA"
                     /locus_tag="ACHKIK_000253"
     CDS             52198..53613
                     /gene="tnaA"
                     /locus_tag="ACHKIK_000253"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO9081492.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMSLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            53704..54951
                     /gene="tnaB"
                     /locus_tag="ACHKIK_000254"
     CDS             53704..54951
                     /gene="tnaB"
                     /locus_tag="ACHKIK_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO9081493.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            55083..56258
                     /gene="mdtL"
                     /locus_tag="ACHKIK_000255"
     CDS             55083..56258
                     /gene="mdtL"
                     /locus_tag="ACHKIK_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO9081494.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            56233..57192
                     /gene="yidZ"
                     /locus_tag="ACHKIK_000256"
     CDS             56233..57192
                     /gene="yidZ"
                     /locus_tag="ACHKIK_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO9081495.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            57349..58098
                     /gene="yieE"
                     /locus_tag="ACHKIK_000257"
     CDS             57349..58098
                     /gene="yieE"
                     /locus_tag="ACHKIK_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO9081496.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            58120..58686
                     /gene="yieF"
                     /locus_tag="ACHKIK_000258"
                     /gene_synonym="chrR"
     CDS             58120..58686
                     /gene="yieF"
                     /locus_tag="ACHKIK_000258"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO9081497.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(58740..60077)
                     /gene="adeP"
                     /locus_tag="ACHKIK_000259"
     CDS             complement(58740..60077)
                     /gene="adeP"
                     /locus_tag="ACHKIK_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO9081498.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            60244..60909
                     /gene="yieH"
                     /locus_tag="ACHKIK_000260"
     CDS             60244..60909
                     /gene="yieH"
                     /locus_tag="ACHKIK_000260"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO9081499.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            60976..61449
                     /locus_tag="ACHKIK_000261"
     CDS             60976..61449
                     /locus_tag="ACHKIK_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081500.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELYENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            61498..62085
                     /gene="cbrC"
                     /locus_tag="ACHKIK_000262"
     CDS             61498..62085
                     /gene="cbrC"
                     /locus_tag="ACHKIK_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFO9081501.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(62147..62869)
                     /gene="yieK"
                     /locus_tag="ACHKIK_000263"
     CDS             complement(62147..62869)
                     /gene="yieK"
                     /locus_tag="ACHKIK_000263"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO9081502.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALD"
     gene            complement(62884..64053)
                     /gene="yieL"
                     /locus_tag="ACHKIK_000264"
     CDS             complement(62884..64053)
                     /gene="yieL"
                     /locus_tag="ACHKIK_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO9081503.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(64080..65696)
                     /gene="bglH"
                     /locus_tag="ACHKIK_000265"
     CDS             complement(64080..65696)
                     /gene="bglH"
                     /locus_tag="ACHKIK_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO9081504.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(65782..67176)
                     /gene="bglB"
                     /locus_tag="ACHKIK_000266"
     CDS             complement(65782..67176)
                     /gene="bglB"
                     /locus_tag="ACHKIK_000266"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO9081505.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDSVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(67195..69072)
                     /gene="bglF"
                     /locus_tag="ACHKIK_000267"
     CDS             complement(67195..69072)
                     /gene="bglF"
                     /locus_tag="ACHKIK_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO9081506.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(69206..70042)
                     /gene="bglG"
                     /locus_tag="ACHKIK_000268"
     CDS             complement(69206..70042)
                     /gene="bglG"
                     /locus_tag="ACHKIK_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO9081507.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(70328..71053)
                     /gene="phoU"
                     /locus_tag="ACHKIK_000269"
     CDS             complement(70328..71053)
                     /gene="phoU"
                     /locus_tag="ACHKIK_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO9081508.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(71068..71841)
                     /gene="pstB"
                     /locus_tag="ACHKIK_000270"
     CDS             complement(71068..71841)
                     /gene="pstB"
                     /locus_tag="ACHKIK_000270"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO9081509.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(71932..72822)
                     /gene="pstA"
                     /locus_tag="ACHKIK_000271"
     CDS             complement(71932..72822)
                     /gene="pstA"
                     /locus_tag="ACHKIK_000271"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO9081510.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(72822..73781)
                     /gene="pstC"
                     /locus_tag="ACHKIK_000272"
     CDS             complement(72822..73781)
                     /gene="pstC"
                     /locus_tag="ACHKIK_000272"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO9081511.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(73868..74908)
                     /gene="pstS"
                     /locus_tag="ACHKIK_000273"
     CDS             complement(73868..74908)
                     /gene="pstS"
                     /locus_tag="ACHKIK_000273"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO9081512.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(75156..76229)
                     /gene="lpfD-O113"
                     /locus_tag="ACHKIK_000274"
     CDS             complement(75156..76229)
                     /gene="lpfD-O113"
                     /locus_tag="ACHKIK_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFO9081513.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPSPGQFTATATITVEIVR"
     gene            complement(76240..78762)
                     /gene="lpfC-O113"
                     /locus_tag="ACHKIK_000275"
     CDS             complement(76240..78762)
                     /gene="lpfC-O113"
                     /locus_tag="ACHKIK_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFO9081514.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVST
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(78787..79518)
                     /gene="lpfB-O113"
                     /locus_tag="ACHKIK_000276"
     CDS             complement(78787..79518)
                     /gene="lpfB-O113"
                     /locus_tag="ACHKIK_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFO9081515.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(79566..80138)
                     /gene="lpfA-O113"
                     /locus_tag="ACHKIK_000277"
     CDS             complement(79566..80138)
                     /gene="lpfA-O113"
                     /locus_tag="ACHKIK_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFO9081516.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT        19978 a        21033 c        19679 g        19518 t
ORIGIN      
        1 taacatgctt ttgctaataa tctcaattgc tggccctata atattgcgct agcattgctt
       61 tctggttgta tcagcgatac tcaaaaattc ttaatacaat actcattcga ctggtactta
      121 tttgtaactc agttatattt tgtcgcccgg tgattcagaa gaatgcaaaa aacggctacc
      181 actccatcaa aaatacttga tctcactgcc gcggcatttt tacttgtcgc ctttctgacg
      241 ggtattgcgg gcgctcttca gactcctacc ctaagtatat tcctcggaga tgaactgaaa
      301 gcccgtccta taatggtaga ttttttcttc accggtagcg ctattatggg aattctggtc
      361 agtcaatttc tggcaaggca ctccgataaa caaggcgacc gtaaattact gattctgcta
      421 tgttgcttat ttggagtgct ggcctgcacg ctttttgcgt ggaatcgcaa ctacttcatt
      481 ctcctctcaa cgggcgtact tctgagtagt tttgcttcca ccgcaaaccc gcaaatgttc
      541 gccctcgccc gtgaacacgc cgacagaaca ggccgtgaga cggtcatgtt cagtacattt
      601 ttacgtgctc agatctcgct tgcctgggtt atcgggccac cgctcgctta tgaactggca
      661 atgggattta gttttaaagt gatgtatctc accgctgcca tcgcatttgt tgtttgcgga
      721 ctgatagtct ggttgttttt gccatcaata caaagaaata ttcctgtcgt tacccaaccc
      781 gtagaaattt taccctccac ccacaggaag cgggatacgc ggctactttt tgtggtctgt
      841 tcaatgatgt gggcggcgaa taatctctac atgataaata tgccgctatt tattattgat
      901 gaactgcatc taaccgataa actggctgga gaaatgattt gtatcgctgc cggtctggaa
      961 attccgatga tgttaatcgc aggctattac atgaaacgta ttggcaagcg actattaatg
     1021 ctcattgcta tcgtgagtgg aatgtgtttt tacgccagcg tactcatggc gacgactccg
     1081 gcggttgagc tggaattgca aattcttaat gccatcttcc ttggtattct ctgtggtatc
     1141 ggcatgcttt attttcagga cttgatgcct gaaaaaatag gctctgcgac aacgttatat
     1201 gcaaatactt cacgcgtcgg ctggattatc gccggctctg ttgacggaat tatggttgaa
     1261 atctggagct accatgcgtt gttctggctg gcgataggga tgttgggtat tgcgatgatt
     1321 tgcctgctgt ttattaaaga tatttagctc accagtctgg gctaagaaaa atcggcgtgg
     1381 cgcaaacaga gccatcctcg ccatttttgc tatcatgcct gcatacataa acgacaaaac
     1441 agtatgcaga gggaaaaatg ggttccacca gaaaggggat gctgaacgtt ctgattgccg
     1501 ccgtgttgtg gggaagttca ggggtctgtg cgcaatacat catggagcaa agccagatgt
     1561 cgtcgcagtt tttgactatg acgcgtttga tattcgccgg tttgattcta ctgacgctgt
     1621 catttgttca tggcgataaa atcttttcta ttattaacaa tcataaagat gccattagcc
     1681 tgctgatttt ttccgtggtt ggcgcgctaa ctgtacagct cacttttttg ctaaccatcg
     1741 aaaaatcgaa cgcagccacg gcaacggtgc tgcaattcct ctcaccgacg attatcgtcg
     1801 cctggttctc actggtgcgt aaatcgcgcc cgggcattct ggttttctgc gctattttga
     1861 catcgctggt cgggactttt ttattggtga cacacggtaa tccgacgtca ttatcgatct
     1921 ctcctgccgc gttgttctgg ggcattgcct cggcatttgc tgctgcattc tataccacct
     1981 atccctcaac actaattgcc cgctatggca cgttaccagt cgtcggctgg agtatgctga
     2041 ttggcggtct gattctgttg cctttttatg ccagacaagg aacaaacttt gtcgttaacg
     2101 gcagtttgat tctggcgttt ttttatttga tcgtcattgg tacgtccctg acatttagtc
     2161 tgtacctgaa aggagcacaa ttaattggcg gtccaaaagc cagcattttg agctgtgcag
     2221 aaccattaag tagcgcgcta ctctctttgc tgttgctggg gatcacgttc acattaccgg
     2281 actggctggg aacgctgcta attctgtcat cggtaatttt gatttcaatg gattcccgtc
     2341 gccgcgccag aaaaataaat cgtccggcgc ggcatgagtg agaattacca gccaggcacc
     2401 gcgccaccgt taaaaatggt ttctgccgct ttagcgactt cgggtgattg ataagattgc
     2461 agaaattcct tcacgttttc tgcattctta ttatcttccc gtgccaccaa aatattcaca
     2521 tacggcgaat tcttatcttc aataaatacg ctgtcgtgca ccggagaaag ccctgtctgc
     2581 tgaatgtaag tggtgctgat aatcgctaca tcaactttgg gatcatccag tacgcgcggg
     2641 agctgcgccc cctcgagttc cataatctgc aaatgacgag gattatcggt tatatccagc
     2701 gcggtaggta ataacccttt tccctctttc agagtaatta gcttctcttt ttgcaacagt
     2761 aaaagtgcgc gaccaaggtt ggtcgggtca ttggggatcg ccactgtcgc gccttctttt
     2821 atttgcgcca cggttttgat ttttttggaa taacccgcca tagggaagac aaacgtattt
     2881 cccaccgcta ccagtttata accatgcgcc tgattatctt gttcaagaaa gggtcgatgc
     2941 tggaagacat tcgcatcaag ttcgccatga tttgtcgcat cgttcggtag caacgaaccg
     3001 ctaaatccta ccagttcaac atcgaggcca tatttctctt ttgccacctt tttagcgact
     3061 tccgcgacat cttgttctgc gccatttata acgccaactt taatgtgttt tgcatcgctg
     3121 ctactctggt cgcaacctgc cagcagaatt ccggccagca ataatgcggc ccctgtccgt
     3181 agatgatgtg ttgtcagttt cattttttta tccttttatt aatgattacc tgactatagc
     3241 ggtaaggtcg ctgcggttta aaaaacgaaa cgctatcgat aagaataaaa aggaataaaa
     3301 gtggaatata aagtctggca cttcttactg accacgcaag cgcgtttcgt acagcatgat
     3361 gagagcgatg aaagcaaact acacttatgc ttcattcgat acacatttgt aaaaggttga
     3421 atccatgaaa ccaacgacac tactgcttat tttcactttt ttcgctatgc caggcatcgt
     3481 atacgccgag tcgcctttta gctcactgca gtccgcaaaa gagaaaacca cggtattaca
     3541 agatttacgt aaaatttgca cgccacaggc gtcattatca gatgaagcgt gggaaaagtt
     3601 aatgttgtca gatgaaaaca ataaacaaca tatacacgag gccatcgtgg cgatggaacg
     3661 taataatcag agcaattatt gggaagcgtt gggtaaggta gaatgcccgg atatgtaaga
     3721 acagcaacaa ctactgacca acgcttcttt acctgatatc aggatttctt cattttcacc
     3781 tttgcagtaa ccaacaatgc agtcagcaac atcaatgtgc cggacaacat caacggcgaa
     3841 gtcagaccaa tattatccag cgcataaccg ccgattgccg cgccacaggt attagcaagc
     3901 tgaataaccg ccacctgaat agacccggct ttttctgcct gatcggccag cgagcgggtg
     3961 atccacgttg accagccgac gggaaccaat gcaaaagtta gcccccagat aatcgccacg
     4021 ccggtagcaa cgattttatc gcttccccac aacgtcagca ccaacgcact cacagccagt
     4081 attaacggcg cgcctgctaa ggccagtttt accgaacgtt taagaatgaa tgacgaaagc
     4141 gacgtaccga taaagctggc aatgccaaaa ctcaacagca ccagcgttaa gccatccacg
     4201 ctgaatcccg ccaggttcat atacaccggg cgaatatatg tgaagaaagc aaactgcccg
     4261 gcgaaagaca tgaagatggc gatcatccct gccatcacac ccggacgttg taataagcgg
     4321 aaagtatttt gtttctgatg cgagggttcg cctggcagtg aaggcaatga tttgataatc
     4381 cagaaaatac acagcacgcc catcaccgcc gccgcattaa agacattgcg ccaaccgata
     4441 agctcgccta aaaaactgcc caacggcgcg gcaatcacca gcgcaataga aaccgcgccg
     4501 aagatcaccg acagcgcctt cggcaccgta cgcggcggca ccagacgcat ggtcagcgac
     4561 gccgacatcg cccagaaccc gcccagcgcc agccccagac aggcacgacc gattaaaagc
     4621 aaactgaatg agttagcaaa ggaaaccagc aagcaggaga gcgtcagcaa aacggcaaac
     4681 aaaataacaa cgtagcggcg gtcagtagcc tgaattgtct gggtaataaa caaactggca
     4741 aacattgcca caaaggcggt cacggtcacc gattgcccgg caaccccttc cgaaatgcct
     4801 aaatcctggg ccattggcgt caacaaactg acgggcaaaa actcaacgat aatcagacag
     4861 gcgacacaaa acgccaccga gaaaacggct gaccagttcg gtcgggtgat ggcatccgcg
     4921 ccgcggtttt cggcaataaa ttcactcatg gtgttacccg tggcatgttt caggggaaaa
     4981 gccgaccact ttaacattga aatgtgtgac gcatttaacg tttttgcaac ttttacggtg
     5041 ttgcttgagc tggagttaag atcgaaacgg aacagggcaa agattaccgg atgcggcgtg
     5101 aacgtcttat ccacccgaca ggtcaggcgc ggtctgtagg ctgataagac gaaacggttt
     5161 caatgcaacc agtgaacgcc ctcggcaggc tgaaagaacg cgttatagcg catctgaaaa
     5221 gcattaatat cctgcatttc aggttcggtt tcgcgcagaa aacctagcgc cagcctgacc
     5281 tgagcgtcgg taatcggcgc ggcgaggcga gctttttgta aaatccgatg ccattgcagc
     5341 aggttctgtt cgctaccatc gacaatgaca atttgccaga tgagcaacac ctgcgcggcg
     5401 ttttgcaggt gcgactcatc cggacgatgc aaataacgta aaaattcgtc accagctgac
     5461 tttcccattt gatcgaccat cgactccacg ggtacgacat cgatgttcag ccgatcgctg
     5521 agacggcgaa tcgcttttct ggccccggat gtcagcaccc gaaaaccaac cacaaacact
     5581 accgccagcg tggcgagcat tatccagatc atgagttctc ctgcgtaatc agaactcatg
     5641 atatctggaa accctcgcca gttactaatc caccagaata atcttcagtg caaacagagc
     5701 tgcgaccacc accacacaca ggttcagatc gcgccagcgc ccggtgcata ctttcatgat
     5761 gcagtacgac ataaagccga gtgcaatccc ttcggtgatc gagaaagtaa agggcatcat
     5821 caccgtggta ataaacgcag gcaccgattc ggtaaaatca tcccagttaa cgcgcgccag
     5881 gctcgaagtc atcagcacgc caacaaagat taacgctccg gcggttgcgt aaggaggaac
     5941 catcgccacc agcggtgaga agaacataac taacaggaac ataacgccaa ccacaaccgc
     6001 agtcagcccc gtgcgaccac cgactgccac accagaagta ctttcaatat aggcagtaac
     6061 agacgaggtg ccgataaacg cccccgccac cgaactgacg ctatcaacat acagcgcctt
     6121 attcatattg gggaatttac cgttactatc tattaagccc gctttatcag ttacaccaat
     6181 taatgttcct gatgaatcaa ataggttgat cagcataaag gagaaaatga taccggcgag
     6241 ttcaagtgtt aacgcgccgc tcaaatctac ttcaccaatg acgccgctaa tatcaggcgg
     6301 aatggaatag acgccgctaa aatgaacatc accgaaaaat aatccacagc aagacgtcac
     6361 cacaatagaa accagcaccg cggcatgaaa atgacgtgat gacaacacgg ttataataaa
     6421 aaaccctaaa atacctaaca acacgccgtg agaacttaaa tcgccaatca tcaccagcgt
     6481 gtctttattg gcgacaataa cgccagtatt ttttaatccc attaaggcga taaataatcc
     6541 aattccgctg gtgataccaa tacgtaaact taagggaatg ttggagatca tccagtaccg
     6601 gatacgaaac agcgtgagca aaaatagtcc aactgcgccc cagaatattg cgcccatccc
     6661 ggtctgccag gagatgccca tcgcccccac gaccacgaag gcaaagaagg cgttcagccc
     6721 cattgccgga gccagcgcca cgggtaagtt agcaaatatc cccatcgcaa tactgccgat
     6781 accggcaatc aaacaggtgg taacaaacac cactttcggg tccatttgtg ccgcgccgag
     6841 gatttgcggg ttcacaaaaa cgatgtacac catggttaaa aaagtggtca tccccgcaat
     6901 caattctgtg cggacggtgg tcccatgctg aggtagttta aataatcgcg aaagcgtccc
     6961 tgattcatta ctcacgtaat cggtattgtc attattcatt tttttgtcca ttggaggaga
     7021 tttaatccct tgcttttgta aaacaaatga caacaacggt tcagtgataa ttatcacatt
     7081 tcaattgcac attaatggat attctttaat aatctcgcga cgtttcttta tgataaataa
     7141 taatcaaatt gataaaatca aaatgagaaa aatatgaata attctattaa ccataaattt
     7201 catcacatta gccgggctga ataccaggaa ttgttagccg tttcccgtgg cgacgctgtt
     7261 gccgattata ttattgataa tgtctctatt ctcgacctga tcaatggcgg agaaatttcc
     7321 ggcccaattg tgattaaagg acgttacatt gccggtgttg gcgcagaata cgctgatgct
     7381 ccggctttgc agcggattga tgctcgcggc gcaacggcgg tgccagggtt tattgatgct
     7441 cacctgcata ttgaatccag catgatgacg ccggtcactt ttgaaaccgc taccctgccg
     7501 cgcggcctga cgaccgttat ttgcgaccct catgaaatcg tcaacgtgat gggagaagcc
     7561 ggattcgcct ggtttgcccg ctgtgccgaa caggcaaggc aaaaccagta cttacaggtc
     7621 agctcttgcg tacccgccct ggaaggctgc gatgttaacg gtgccagttt tacccttgaa
     7681 cagatgctcg cctggcggga ccatccgcag gttaccggcc ttgcagaaat gatggactac
     7741 cctggcgtaa ttagcgggca gaatgcgctg ctcgataaac tggatgcatt tcgccacctg
     7801 acgctggacg gtcactgccc gggtttgggt ggtaaagaac ttaacgccta tattactgcg
     7861 ggtattgaaa actgccacga aagttatcag ctggaagaag gacgccggaa attacaactc
     7921 ggcatgtcgt tgatgatccg cgaagggtcc gctgcccgca atctcaacgc gctggcaccg
     7981 ttgatcaacg aatttaacag cccgcaatgc atgctctgta ccgatgaccg taacccgtgg
     8041 gagatcgccc atgaaggaca catcgatgcc ttaattcgcc gcctgatcga acaacacaat
     8101 gtgccgctgc atgtggcata tcgcgtcgcc agctggtcga cggcgcgcca ctttggtctg
     8161 aatcacctcg gcttactggc acccggcaag caggccgata tcgtcctgtt gagcgatgcg
     8221 cgtaaggtca cggtgcagca ggtactggtg aaaggcgagc cgattgatgc gcaaacctta
     8281 caggcggaag agtcggcgag actggcacaa tccgctccgc catatggcaa caccattgcc
     8341 cgccagccag tttccgccag cgactttgcc ctgcaattta cgcccggaaa acgctatcgg
     8401 gtcattgacg tcatccataa cgaattgatt acgcactccc actccagcgt ctacagcgaa
     8461 aatggttttg atcgcgatga tgtgagcttt attgccgtac ttgagcgtta cgggcaacgg
     8521 ctggctccgg cttgtggttt gcttggcggc tttggactga atgaaggtgc gctggctgcg
     8581 acggtcagcc atgacagcca taatattgtg gtgatcggtc gcagtgccga agagatggcg
     8641 ctggcggtca atcaggtgat tcaggatggc ggcgggctgt gcgtggtacg taacggccag
     8701 gtccaaagtc atctgccgtt gcccattgcc ggcctgatga gcaccgacac ggcgcagtcg
     8761 ctggcggaac aaattgacgc cttgaaagcc gccgcccgtg aatgcggtcc gttacccgat
     8821 gagccgttta ttcagatggc gtttctttct ctgccagtga tccccgcgct aaaactaacc
     8881 agtcaggggc tatttgatgg cgagaagttt gccttcacta cgctggaagt cacggaataa
     8941 taaaaaagcc cggcgtcatg ccgggcaaaa gtcaccagtt acgtttatgc cactgtcaac
     9001 tgctgaattt ttttctcgcg gcggattttg cgttcttcca tcaccgccac tatcgccatc
     9061 aggcagatac aaccaatcgc ggcgatatca agtgcggcga aggtgcctgc ccagccggta
     9121 aggccgaata ccggcgtccc atcggcaatc attcccagac ctaacttggc aaagctgtca
     9181 ccaatcaggt aagcaaaggt gcctttaata ccatcggcag cgccaatcgc ttttttaggt
     9241 acaaagccaa cagcagccac accaatcaac aattgcgggc caaagaccag gaaacccaac
     9301 gcaaagagag aagccagata gatatattcg ttgctggcat gttgatacac accgagcgtg
     9361 gcgataatca gcgccagcgc gatgcaggcc accaggccac ggcgaccgtt cgccaggtca
     9421 gagagccagc cccacagcag cgtaccgacc agtgcaccag cttcaaacag cgtaaagccc
     9481 tgaatcgcca ccgctttaga gagtttcagt tcctggaacg cgtatacggt tgaccactgg
     9541 tcgataccaa tacgtaccac atagaggaaa atgttggcga agcacagcag ccagatcact
     9601 ttgtttttca gcacatactc aacaaagatc tgccacttgg tcatatcggt agattctgtc
     9661 tctttgtcct cttcgctgat ctcctcgccg aacagttctt cagctttgcc gaggccataa
     9721 gattccgggg agtcgctgcc gtaacgcagg ccgataaaac cgacaatcag cgcgataatc
     9781 gacgggaaga taaacatgcc gatgacatgg ccatcgaaca ggtaatttgc cccgaacagc
     9841 gccacacctg ctgcgcctgc accgccaagg ttgtgagaaa tattccagaa accgaggaat
     9901 gtcccgcgtt tacgacgcgg cgtccatttg gtgatggtgg agtaactgca cgaaccgccg
     9961 gtactctgga aaaagccgct taaggcgtag aaggcaatca tcaggaacag gctaaccgag
    10021 ccgctgccca tactggcact gaagcccagc atacaaatgg cagacaggat cagcatgaac
    10081 ggcaggaatt gtttggtgtt tttgccgtcg gcgtagtagg aaaccagcgt tttacccacg
    10141 ccataagtga tggagaaacc caggccgatc atccccagct gcgtcatgct caacccgtag
    10201 gtcgagatca tgtcgttctg cgcgatgtta aagttcttgc gaatcaggta catcgtcagg
    10261 tagccgataa agaccaccag gtaggattgc atgaacggtt tgaaccacat tttgcgccgc
    10321 acttcgagcg gaaggtccag ggtcggcttg cgaacctggt ttaagaaagc cagcatgggt
    10381 tactcctgat ttcccttcat acttcaagcc gcaggtgcct tagctgcacg cattccttcc
    10441 ggtcacatag ttcactatgc tcccgggact cacgcgtttg ctgcgttcct gcaacttgaa
    10501 ttattttggg aaagaattaa tgaatgatgg cattgtaaaa atcagcagca aatcaggcct
    10561 gagatagcgt ccaggcaaaa cctggaaaat ttcttagttt tgccggatat gaagtgaaaa
    10621 ggtgagatgc atcacgcttc gcgcggtgcc tgggcgttca aaaagggcag taacagcagt
    10681 gcggaaaccc cggcggcgat agagataacc acaaaaaatc cgctccagtg ccaggtatcg
    10741 agtactttcg ccagcggcca accagcaagc gacgccccca gataagcaaa caagccgaca
    10801 aaccccgtcg ccgcccctgc agcctctttg tgggaacact ctgccgccgc catgccgatt
    10861 aacatctgcg ggccaaagac aaaaaaacca atggtgaaga agcaggttgc ctgcatcacg
    10921 tagctggcaa atggcatcag ccacagcgag ccgactgaaa gcaaaattcc ggcggcgaaa
    10981 atcaaattca tcggcccacg gttgccgtta aacaatttgt ccgagcccca accggctacc
    11041 agcgcaccga taaatccgcc cagttcaaac atcgtcactg ccgtattcgc cgtgaccaga
    11101 tcgacgccca gcgtctcgga catatacaaa ttgccccagt cgttgattgc cgcccggacc
    11161 acatagacca gcacatagca aaacgaaagc agccagatat acggattcag caacacatat
    11221 ttggtcagga tctctttacg cgtcaaccct gccccttctt gttgttgagc aatttccagc
    11281 gcgtcgtgcc gccattcacc gaccgccggt aaacctaacg cctgcgggcg atcgcgtagc
    11341 cgccagcaga gaaaaatccc cacgactatc gccatacaac cagcaatcat catcccggca
    11401 cgccagccgt aatgcagcgc gctcgctgcc atcacaatgg gaatgagtgc gccgccgacg
    11461 ttatgcgccg tgttccataa tgcccaccaa ccgccgcgct cggtacgtga ataccaggcc
    11521 gttaacagac gcgcacacac cggtgaaccc cagccctgga aaaaggcgtt cagcacccag
    11581 agcacggcaa acgcccatag cgacgtcgag aagccaaaca gaatgttcat gatgcccgtg
    11641 gcgataagcc ctatccccat aaaataacgg gcatttgagc gatcgctgac aatgccggag
    11701 acaaacttcg acacgccgta agtaatgtaa aacagggtcg ctaacaggcc gatatcgcta
    11761 cggctgagca cgccgttagc aaggatttct ggtaccgcgg cgttaaaact tttccgcgtg
    11821 aagtaaaaca gcgcgtaacc cagccagatg gtcagcagaa tatgccgacg ccagtagcga
    11881 tagcgggcat caatttcata tttatcagtc attaatggcg catcggcagg cgctttcaga
    11941 aacggcaaca tcatcaaacc ttagacatag cgttgaggta gagaaacgct gacacgcgtg
    12001 ccgtgcagac aggaaatgtg taatgtgcca cccagcgccg ttacgcgctc gcgcattccg
    12061 gtgaggccaa aaccttgttg cccggaaccc ggcggcaaac cgctgccatc gtcttcaata
    12121 accagcatca accgttcatc ctgctgccag ccttgcaggg tgaccgcgct ggcatcagca
    12181 tgtttcacaa tgttgttcag cccttcctgg cagacacgaa acagcgtcac gcgctggttt
    12241 tcgcttaacg ctgattcatc gattcgccat tcgagatggc tgacaatacc gcgcccttcc
    12301 agctccattt cccgcatcag tgagcggatg gcctgctcca gggtgagatc atccaactgg
    12361 cgcggacgta accgacccaa caaacggcgc accgcgtcgt aaacgcccag cgatagttgt
    12421 tcgatgagct gcccgctctg cttcacgctg gcgttatctg ccgccagccg ctgaacaatg
    12481 cccgcctgag tacgaatggc tgtgatggtc tgaccgatat catcatgcag ctcacgcgcc
    12541 acatcacggc gcacgctctc ttcggtttcc agcaaccgtt cagccagatg ctgattgcgc
    12601 gccagttcct tttgcagcga ctggttaagt tcacgcaacc gctggatgcc agcgccaagc
    12661 aacaaccctg tcagactttg caccagcagc gagagcaata aatccaccgg atgatcgcgc
    12721 caggtttgac tggcgatcag cgcgatggcg ttcatcaacg tcgcaatcag cgccccttgc
    12781 caaccatagt gccaggccag cgcgataatc ggcagcgcca gacaaaatgg cgtaaagcgc
    12841 gacagttcgt ccggcaatcc caactggagc cagagactga taacaaacag cagcaagtac
    12901 cagaccagat gtcgcccgcg ccagttgatc ggctgagaaa ccagtgacgg gccgagcggc
    12961 agccaggtgt tattggcgag atagtgccag aacaccagac atatcggggc cagcgtcagg
    13021 ccgccagtta aagtcagcaa cagcgcattc cacgactctt tgccgtgcca aagccagggc
    13081 agcgactgca acaacgccgc cgccgttaac gccgcaccct gtaacagcaa ggtgcgccag
    13141 tcacgctgat ggcgatagcg cgagatcagc gccacgggca gtaacgtcag taaactaccg
    13201 atcatcaata acggaaaatg ggttaaaccg accgcctgcg ttagccagta aatcagcagc
    13261 cactccgcgc ccagcagcac cggccagtat ccgcgcgggc attgcagcat tagccccaga
    13321 cgcagaccaa acggaaataa cagcaccgcc atatcagggc gctcaaccag gtgcaggctg
    13381 atactccaca ggcaaaacca tgcggcagag aagataaaaa agcaggcaat aacggtaatt
    13441 aagcgggaga acaacgtctt catcaccagc catcaaacat gcggcgcgcc agctctacgt
    13501 cgttactgac acccagtttt tccatcaggt tggcgcgatg gacgtgcacc gttttcggcg
    13561 acaagcccag ttcggcagca atctctttca ccgccattcc ttgcgccagt ttttccgcca
    13621 cctgccgttc gcgtttggtt agcgggtcct gacgaccgga tgccagttta atggcaatat
    13681 ccggcgtcag ataacagccg cccgtggcaa ccgtatgcac cgccgcaatc agttcgtcag
    13741 ggctacagcg tttggagaga aagccgcgtg cccccgcgtt aagcgcctgc tcaaccagcg
    13801 ccggactgtc atgaacagag agcattattg tcgccatacc tttcggcagc tggcttagca
    13861 gttccagacc ggagatatcg ggcatggaga tatcgcaaat acacacctgc acaccgcgcc
    13921 ccggcagccc cgccagcgcc tcgcgccccg aaccaaactc ggcaactacc tgcaaatcag
    13981 gttccagccc cagcagctgc gcaaagccgg agcggacgat gaggtgatcg tctataaggg
    14041 caacggtgat catggtcttg tcctggcggg taaaaaaaaa cgcgcttacc ttaacgataa
    14101 gcgcgatgtt gttcaagcct tgagcggtta ctgaaaaaac accgcaatct tgttaaacat
    14161 agtcggatcg gactgattac gctgcacttt caccacatct tccagcttat cgatttggct
    14221 tatcatctgc tccagacgct ggtcgtcatt gaccagtagc cagatatggc ttttgtcgct
    14281 gtcctgaatc ggcagacaaa gaatgccttc aacgttaaaa gcgcggcggg caaaaaggcc
    14341 acaaacgtgg gtcattacgc ccggatggtt gcgaacggtg agctccagaa tcacgttgtc
    14401 atgagttgtg ttttgcatgg cttattcccc caccatttca gtattcgccg cacctggcgg
    14461 caccatcggg taaacttttt cttcggcatc aatgcgcaca tggatcagcg ccgggccagg
    14521 gcgattgatg atttcctgca atgcagcctg cggatcggct tcgttattca aatcacaggt
    14581 ttcgaggccg aatccggcgg caatctgcat aaagttgatt tttcccggat aggtggcggc
    14641 aaaaacgcct tgctcgtaga acagactctg ttgctgatgc accagcccca gcgcttcgtt
    14701 gttcatcaga atgattttga catccagctg attttcactg gcggtcgcca tctcctgaat
    14761 attcatcatc aggctgccgt cgccggagaa acacaacact ttgcgatccg ggttcgccag
    14821 cgccgcgcca atcgccgcag gcaggccaaa acccatcgtg cccagcccac cggaggtcag
    14881 ccactggcgt gggcgattga gcggataagc ttgcgcggtc cacatctgat gctgacccac
    14941 atcggtggtg ataatcgcat tgtcatcgac acaggcggca acggcgttga tcaggccgta
    15001 atggcttaat ggatcgcacg ctttcgggat tggacacggg aattcgcgct gcaaatccgc
    15061 taccaactgg tgccactctg cacgcggttg cgcttccacc tgcgggatca attgcgccag
    15121 cacgtcatca acatccgcct gaatcgccac atgcggctgc ttgattttac ccagctctgc
    15181 gcgatcgata tcgacatgaa taattttggc attcggacag aactgctcgg ttttgccaat
    15241 cgcccggtca tcaaaacgcg caccgagcac tatcaacaaa tccgcctcct gcaaaatata
    15301 gttggtgctg cgcacgccgt gcatccccag catacccagc gacaacggat gcgcttttgg
    15361 caacatgccc agcgccatta aagtcatggt ggtaggcagt tgcgctttct ccgccagttc
    15421 acgcacccgt gcgggcgcat tgatcacacc gccgcccaga taaagcaccg ggcgtttggc
    15481 agcgttaatc atcgccgctg cgtcacgaat gctttcttcg ctaaaggcgg gggcggcggc
    15541 tttttctgcc atagcgggct gtgtttcaat ctcaaaaact gccgtttgca catccttagg
    15601 aatgtctatc cacaccgggc ctgggcggcc tgattgcgca atgcggaagg catcgctcat
    15661 gacctgcggg agttcttcga tatgtctgac cagatagttg tgtttggtga tggggataga
    15721 gatgccgtag gtgtccactt cctggaagac gtcggtgccg atcatcgagg cgggaacctg
    15781 accggtgatg caaatcagcg ggatggagtc cagccgcgca tcggcaatgg cggtcaccag
    15841 gttagtcgca cccggtccgc tacaggccat acagaccgcc ggtttaccgt cggtgcgcgc
    15901 catcccctga gcgataaatc ccgcgccctg ttcatgacgg gccagaatat ggcggatttg
    15961 cgtgctttgg cttaaggcat cgtaaacagg caggatagaa ccgcccggaa tgcccgtcac
    16021 aatcttaatg ccctgctgtt ccaggaaatg aacgataaat tctgcgccgg taaagcgctt
    16081 acgcgtcgat gttgtgcccg aacttgccat gctccagtcc ttttcttctg ggccgacttt
    16141 ccgggaggtg cttaaacgaa aaaccccgcc cggtttgcgc cggcggggtt ttggaatcgt
    16201 gtgttgttcc agtccctacg gcgcattgcc gacgaccacc accacacgca cgacgaccac
    16261 tgcggcggat agcgcagttg gtagtagttt tgcgttgaac atggaagtag tcattgggga
    16321 ccttgttggt tttgtgttta acaatattta tacaagcaca gctttacagg ggagacaatg
    16381 gaaaattttt cagcaaggga aaattgaggg gctgatcacg ttttgtactg aattgcaggt
    16441 aacaaaaaac cccgccggag cgaggtttca tcagccgcct gcggctggta accgcaaagc
    16501 acactgtatt atgtcaacac tgaaagtata cgtgttccgc gcagaacgcg caatttcggc
    16561 acgaattttg acgtatttag ggcatagttg agtatcgatc acagtttgcg ttttgtccaa
    16621 atattactgt ttatttatac agtaaacttc tataatatca ctgtacgcaa tgtgttatgc
    16681 gggggccgca tcgttacccg gcgcactaag tcctggctga aacgggtggt gccgtcagcg
    16741 ccttaacccc gcgtgagcac actgtgttat gtcaacaagc acaacgtttc tccttaagat
    16801 accgcgtgca caacagctgg caacaggcag cggaaaggta cgtcagctgg cagtgctcct
    16861 gaaccacagg agacgcgtat gagcctggtg gatatcgcca ttcttatcct caaactcatt
    16921 gttgcagcac tgcaactgct tgatgctgtt ctgaaatacc tgaagtaatt cagattcaag
    16981 tcgcaccaaa ggggagcggg taaccgctcc ccttttatat ttagcgtgcg ggttggagtt
    17041 gggggcttct tctaccagca gaatgtttgc acctgcgctg cctgtgcccc tcaccctagc
    17101 cctctcccca aaggggcgag gggaccgtcc cgtgcaatgt ttagctcatt ttctgaaagg
    17161 gaacggtggt ttccccctcg cccctttggg gagagggtta cagttcgcac gtaatccctc
    17221 ccggcaacat ttattgccgc ttttgtttac atattctgcc gctaaacaat tccccattcc
    17281 tggcgtatat ctggctaaca ttcatcaatg tgatagattc ctctcccgca tttatgggaa
    17341 tgcgtagtga cttattctaa ttatttttat aaaagcatcc gtgataatga aaaggcaaag
    17401 aaacgtcaat ttgttattga tgttggtatt actcgtggcc gtcggtcaga tggcgcaaac
    17461 catttatatt ccagctattg ccgatatggc gcgcgatctc aacgtccgtg aaggggcggt
    17521 gcagagcgta atgggcgctt atctgctgac ttacggtgtc tcacagctgt tttatggccc
    17581 gatttccgac cgcgtgggcc gccgaccggt gatcctcgtc ggaatgtcca tttttatgct
    17641 ggcaacgctg gtcgcggtca cgacctccag tttgacggtg ttgattgccg ccagcgcgat
    17701 gcaggggatg ggcaccggcg ttggcggcgt aatggcgcgt actttgccgc gagatttata
    17761 tgaacggaca cagttgcgcc atgctaacag cctgttaaac atggggattc tcgtcagtcc
    17821 gttgctcgca ccgctaatcg gcggtctgct ggatacgatg tggaactggc gcgcctgtta
    17881 tctctttttg ttggttcttt gtgctggtgt gaccttcagt atggcccgct ggatgccgga
    17941 aacgcgtccg gtcgatgcac cgcgcacgcg cctgcttacc agttataaaa cgcttttcgg
    18001 taacagcggt tttaactgtt atttgctgat gctgattggc ggtctggccg ggattgccgc
    18061 ctttgaagcc tgctccggcg tgctgatggg cgcggtgtta gggctgagca gtatgacggt
    18121 cagtattttg tttattctgc cgattccggc agcgtttttt ggcgcatggt ttgccggacg
    18181 tcccaataaa cgcttctcca cgttaatgtg gcagtcggtt atctgctgcc tgctggctgg
    18241 cttgctgatg tggatccccg actggtttgg cgtgatgaat gtctggacgc tgctcgttcc
    18301 cgccgcgctg ttctttttcg gtgccgggat gctgtttccg ctggcgacca gcggcgcgat
    18361 ggagccgttc cccttcctgg cgggcacggc tggcgcgctg gtcggcggtc tgcaaaacat
    18421 tggttccggc gtgctggcgt cgctctctgc gatgttgccg caaaccggtc agggcagcct
    18481 ggggttgttg atgaccttaa tgggattgtt gatcgtgctg tgctggctgc cgctggcgac
    18541 gcggatgtcg catcaggggc agcccgttta agcgcacgtc accgcagcat cgtcatcagc
    18601 tccatgggag aacgatgctg ctttatcaga tcacgcatca cccgcatatg cggtgcggag
    18661 taagaataaa acgcctgata gcccgcacaa agcacgctgt ctggcgtctc ctggcgagcg
    18721 tgcgggcaca ggcgatgcca gtcgcacgcc tggcagtcac ggcgcggttg cgggttttcg
    18781 ctgccacacc atttttgtaa ggtggcgtca aaaagctcaa tcgagatacg actgatatct
    18841 gcccgcaccc aacactcgaa cagggcaatt aaaaatttcc cccacagctc tgctgtcacc
    18901 gaagcggccg tcagatcgcc ccggtcatca aattcaagat agggaatgag ctgtacggag
    18961 cggatgcccc gctgtttgaa attgtgataa aaatcggcag gttgcagagc atccttgtac
    19021 tctacaacca gtttatgcct gtcctcttca gcatcaatga cctgacttcc tgtcatgcga
    19081 ctctctcaat aaatacgata agttgatgta tatgcgttac agcaaacagt attgcgagag
    19141 ataacaccgc gagggagatc aaaaatttgt cacggactac gtgaaattgg gaaaaatcgc
    19201 tattcgtgac atccattaaa ttgcgattac gggtgtagtg ccagaggatc agcgccacga
    19261 tggcgagaat gccaatcgaa atccagaata acatgcccgc ctggtgccag ttgtgtttga
    19321 tagccaacgc catcaacgcg ccgtagccca gcatggtacg aaaccacgcc agtgatgtgc
    19381 gctccggctg cagtcctggg tcggcaatgc gtctggcttt gcggctatcc ggcatacaac
    19441 accagtccca taacgatcac cgccacgacc atcaatatta agctaatgat taataagctg
    19501 ttggtgtagg gcaggtcctc tttcagacgc atcgcttttt cattgcgtaa ccagcgcagg
    19561 tagccataca tcgctaaccc gccggaaaac agacacaaca acaacgccag cagttcacga
    19621 atgacgggcg tggcgaaatc tggcgcaagt tgatcaagcc caacccccgc cgccagaaat
    19681 cctagtgcgg tacggatcca cgccagaaaa gtacgctcat tagccagcga gaagcggtaa
    19741 tcgggcgctt ctccaaggcg ggaaattttc attgttgctc ctttgcaatt tcactaacgc
    19801 tctaccttta gcggtttttt tttaagaatt tcgtccgccc gtttcctgat atgctttcgc
    19861 catccgctac aaggaggtca gatgggtatc atagctcaaa ataaaatcag ttcattgggc
    19921 atgctgttcg gtgccatcgc tttaatgatg gggattatcc acttttcatt cggccctttt
    19981 tccgcaccac cgccaacgtt tgaaagcatc gtggcggaca aaacggctga aattaaaaga
    20041 ggtcttctcg ccggaataaa aggcgaaaaa attaccaccg tggagaaaaa agaggacgta
    20101 gatgtcgata aaatcctcga tcaaagcggt atcgcactgg ccattgctgc gctactttgc
    20161 gcatttattg gcggtatgcg taaggaaaat cgctggggca ttcgcggcgc gctggttttt
    20221 ggcggcggga cgctggcttt ccataccttg ctttttggta tcggcatcgt gtgcagcatt
    20281 ttgctgatat tcctgatttt ttccttcctg accggcggtt cactggttta agcagcctga
    20341 ataatgcccg ccgggtgttc atcagcgggc attatcgtca gaacttctgt tttttctctt
    20401 ccactttcac tccttgcgtc ggtaggccgg tgtcatagtc acgtaccacc ggcgaatagc
    20461 catcttgtgg gcgtggacga aatgccccca tccagcgcgg cagtgcatct ttacgccacg
    20521 gacgcaggct ccattggtaa ctgcggaacg gatcgcgaat tttgtccatg taatccaata
    20581 aggcgtcatg cattttgctg cgaacgtctg caaaacggat atcatcgatc aggttatgca
    20641 tttcatttgg gtcattacgg cgatcgtaaa gttcatcact ggtgaagagg ttgagtacca
    20701 gtttaaagtc atccgtcacc cagcaacgca ccggaataaa accgccaaag ctgtcatgct
    20761 caatctcgta gcggttaaat tccaccatta caccgcgtgg ctctttcacg gcaaggatat
    20821 tttcccccgg cagaatctct ggtttttcaa tatctgccag cgccatcatt gtcggcagta
    20881 aatcgatatg actgactggc gtatcgacct gtcgccgctc cccttgcggc gaacggatga
    20941 tcagcggaat acgggtgatg tcgtcataca tcgccgctcc tttactgatc agcttatgtg
    21001 cgcccatcat ttcgccgtga tcggaagtat aaatgaccca cgtattttca cgttgctctg
    21061 gcgttaaggc attgatgacc cgtccgattt ggtcatcaac aaagtcatta caggcaaaat
    21121 agagcggatg gtgataaagc ccgtcatcac cgactggcga tggcatcgcc tgcgcccata
    21181 agcggtgatg ttccggtttg ttcgccaggc catcctgagc tttctcgccc agatcgtagt
    21241 aaaaatcagc gtatttctct aaatactcca ccggacaggt gaacgggtga tgcggctcat
    21301 catacgaaac caccatcagg aagggttcct cggcgcgcgc gggctgttgc agaaaatcta
    21361 ctgcccgatt gctgatgcgg tgcgcccagg tgaaggtttc gtcgatatgg ttcgcctgta
    21421 aatcctcaac gctgtttagg ccattgcgcc acaggctgat ctctttttcc gtcagttcgc
    21481 taaggtagtt cgccccatcg aaccagtaat cagcgtccca ctccggcgga cactcgccag
    21541 tgccgaaata gtcatgaccg tcgagatgcc atttgccgat gtaacaggtg tgatagccgg
    21601 catccttaaa gtagcgcccc atagtggaga tgtttttgcc tggcgcgacg ttgttggtcc
    21661 acgggccgga ctggttagcg tagataccgg taaatagtcc ggcgcgtgca ggcgtacaaa
    21721 ccggtgaaca ggtgtaggcg gaattaaagc gaataccttc cgccgccaga ctatcaatat
    21781 tttgcgtatt cagcggttta ccgctatagc aaccgaccat attggtggcc tgggtatcgg
    21841 tcatgatgaa cagaaaattg gggcgtttca taggctatcc ttcgcgtcgg gagtaaaggt
    21901 tacggctggg tcgtgacgat gccgccagct gtcaaaaatc aggtagacaa tcactacggc
    21961 ggcaatgaaa taactgatca tcgctaacca gcgcgtaccg tagccgccga attcagccag
    22021 cccggcatag acgccaatca tcgcgaacag gatgccaatt gacgcgatct tgacgtttct
    22081 ccacggtttc atgtccaccg caaacgcatc tttgaaggtg aacggcgtgg cgcgcggttt
    22141 gataaaaccg atcaccagca tcacgaccac gttgatgcag aacgtacagg ccagcacata
    22201 gaggaaatgg aagtcgaact tcaccagata gttgatggtg atgtagctga ttatgccaat
    22261 ccccatcgcc acttttgccg ccagcgccgg gatgcgcggg aagaaaaagc ccataatgat
    22321 gatggtaacc agcggcacgt tgtaaatgcc gttgagctgt ttcatccagc tatacagccc
    22381 ctgcggcgcg ttggcgatcc acggcgcgac cagcaccgaa acgatagcga taaagaaacc
    22441 aaatttgcgc ccgacggtga ccagctgctg cggctcggca ttctggttaa tgatgcgacg
    22501 gtaaataccc atactgaata acgtactggc gctattcaga aagccgttga aggtactgat
    22561 caccgcacca aataacaccg cgccgaagaa acccaccatt ggcactggca gaacgttatt
    22621 gaccagcgtc gggtaggcca tgtcggcttt cggtaaatcc tgatacagat gaaacgcaat
    22681 caaccctggc agcaccagta ccagcgggtc gagcattttc agcaccgccg ttaacagcgc
    22741 ccccttttgc ccttccgcca ggctttttga cgccagcgtg cgttgcacga tgccctgatt
    22801 tgtacaccag taaaaggtgt tcaccagaat caaaccggta aatgccgcgc caatcggcaa
    22861 gggatcggtc gggccaccga ttgagtttaa tttctcggcg tgaacggtgg tgagttgctc
    22921 aatgccttgc ataaagctgc ccttgcccat cgcgatcagg ccaaataccg gcaccatcaa
    22981 cccgccgata accagcccaa taccgttgat ggagtccgcc actgccattg cgcgcagtcc
    23041 gccgatcacc gcatacaaaa ttcccgccag accaagcaaa attaccagta gccagatagc
    23101 cgcaccgtga gaaatctgta gcgattcccc gacgtgaaac aggctgttga gcgccaacgc
    23161 gccggagtag agaacaatcg gcagaaagca gacgccggtg gcaatgagga agcagaagtc
    23221 gatgataata cgcgtcgttt tatcataacg ttcctccaga aaatcgggga tggtggcaat
    23281 gccgcgcttc agatagcgcg gtaaaaagat tagcgcgagg aagatcagcg tcaccgctga
    23341 agtcacttcc cagcccatca ccgacatgcc gcttttgtag gcctgcccgg aaaggccgac
    23401 cagttgttcc gtggaaaggt tggttagcat taacgaagcg gcaataaccg gcgcttttag
    23461 tgaacgtccg gcaagaaaat agccttgttg cgatccggta tctgttttgc ggaccttcca
    23521 ccaggtgatc accgccacca gcagcgtaaa accgacaaaa ctcaagattt gtaacgaatt
    23581 catctcatag cccttattta tgtttatgtc ccggacgcat gaatataggg agaggtaaaa
    23641 tgcgtcgggt caggcagtca gattcttaaa atacaaacgt cgtatccctg aacggattgt
    23701 tttctgttaa gttcaggttg tgagcataat tcgccggagg gatattaaaa tgaatggaaa
    23761 attgcaaagt tcagatgtaa aaaacgaaac tccgtacaat attcccttat tgattaacga
    23821 aaatgtgatc tccagcggaa tttctctgat ctcgctctgg catacctacg ccgacgagca
    23881 ttaccgggtg atctggccgc gggacaagaa gaaaccgctt atcgccaact catgggttgc
    23941 ggtgtatacc gtacaaggat gtgggaaaat tcttttaaag aatggcgaac aaataacact
    24001 gcatggcaac tgtattatat tcttaaagcc aatggatatt cactcttatc actgtgaagg
    24061 tttagtctgg gaacagtact ggatggaatt tacccccacc agtatgatgg atattcccgt
    24121 tggtcagcaa agcgttattt ataatggcga aatttataat caggaactca ccgaagttgc
    24181 tgagttaata acttcaccag aagcaataaa aaataatctg gcagtcgctt ttctgacgaa
    24241 aattatttat cagtggattt gtcttatgta cgcagatggt aaaaaagatc cacaacggcg
    24301 gcaaattgaa aaattaattg ccactttaca tgccagtctg caacaacgct ggagcgtagc
    24361 tgatatggct gccacgatcc cctgtagcga agcctggttg cgtcgtctgt ttttacgcta
    24421 taccggcaag acgccgaaag aatattacct cgatgcgcgt ctggatctgg cgctatcgct
    24481 attaaagcaa caaggaaact cggttggcga agtcgctgat acgctcaact tcttcgactc
    24541 ctttcatttc agcaaagcct ttaaacataa atttggttat gcgccgtcag ccgtgctaaa
    24601 gaatacggac cagcacccaa cggatgccag tccacacaat taacgtaact ctggccagta
    24661 cgctttgttg gcctccagca actcatccag aatagctttg gcgaccgagg cgctcggtac
    24721 cgttttcgac aacgtaatcg cctgccacag gtgctgatat gaacgctgtt cccaggcgtc
    24781 cacgaccagt ttttccaccg ccacttgctg actcattaac cctttctgaa actgcgggat
    24841 atcgccgacc accagtggtt caggccgtag tagcgcacgc gcatctgttt gcgatcttgc
    24901 agcccaacaa tttgcgccat ccggctttca ataccgattg gcatgtcaca gatgttgagg
    24961 attttcgcat tcgggcgcag acgacgcgtg gcttctgcga caattgctgc cgggttggag
    25021 tagttgagca tccaggcgtt tggtgaatat ttttccatat aatccaccag ttccaggacg
    25081 ccgccaatgg aacgcatgcc gtacgctatt ccgccgggtc cgcaagtttc ctgaccaaca
    25141 acaccgtggc gcagtgggat tttttcatcc agttcgcgca tcgggtactt gccgacgcgg
    25201 atgtgcgcca tgacaaaatc aacgtcgctg aacgctactt caggatcggt ggtgtaacta
    25261 aacgcaatgt ccggcgcttt ttctttaagg atgactttac aggcttcggc aatcacttcc
    25321 tgccgcgcgc catcgttatc ataaaatttc agtgcacgaa gcgggaaacg gtcctgattc
    25381 gccaggagca tcaacacgat gcctggcgta aaggtgcttc caccgcctgc gacaaccact
    25441 gagaatttgg tcattatact gcctccgtaa tggcaacatt ttctgctgat tgatgagaat
    25501 taattaagct atcgagctgt tcacgcaatt gggatacatg cagaccaatg atcacctgaa
    25561 tggcatcgcc actacggaag acgccgtgcg ctcccagctt tttaaagact tcgtcatcca
    25621 gcgtttgtga catgtcatgc agtgcaatgc gtagacgcgt cgcgcaattg ttaatgctgg
    25681 agatattgcc gaccccgccc agggcttgca gaataccggc agcctgatcc agctcttttt
    25741 ttggctctgc cgcggtggtt tggcctcgcg aggctttgta ttcggctttt gagtagagtt
    25801 tcacttccgc atcttcacgt cccggcgtgc acatgttgaa ctgcagaatc agggtgcgga
    25861 aaaccacgaa gtacagcagg gtaaagcaca acccaatggc gatttgggtc agcatcatat
    25921 ccgcgtggtt gctgaacatc gggatccagt tttgcggtaa aacctggtca atcagacctc
    25981 cgcccatgtt gcccaccaca ccaaagagat acatcacggt cgacattgag gccgccagca
    26041 cggcgtgtac cgcaaacagc aacggtgaaa tgaacaggaa ggtaaattcc agcggttcgg
    26101 taattcccac cagcatggcg gttaaggttg cgggaatcag caagcccgcc acttttaccc
    26161 gattttccgg tgcggcagtg aagtacatcg ctaaagaaat gcccacggca ccaaagattt
    26221 ttgagttacc gtgcagggca aaaccgcctt ccgggaacaa cgatttcagc ggctcggcgc
    26281 tcagactgaa ctcttgcaga tgctgcgccc agtacatctg aatgccgcct tcaacagctg
    26341 ccggaccaaa gataaacggt ccgtagatga agtggtgtaa accggttggg atcagaatac
    26401 gttcgaggaa ggtgtaaacc cacaccccaa gcgcacccgc cgaacgcagg aacgcttgca
    26461 gagattcaat ccccatttgt acttttggcc agccgagcaa cgtcagccag gcacagggga
    26521 tcatcaccag gaaggcgata atcaccacat aagacgtccc ctggaaaatg ccgagaaaaa
    26581 ccggcagttt tttatcgaac agacggttat gcagcgccgt cacaatgccg gaaatgataa
    26641 ttgcgccgat aatgctggta tcgagggttt taatcccggc catcattgtc agaccgctac
    26701 ctgccaccgc gtcctgagtg aaatcgacgc cgaagtagct tccccaggtc attcccatcg
    26761 cgttgatgaa atagttccag gtcaggaaac tcaccatcac cgccagacaa gcacgcccct
    26821 gcgcttgctt agcaaggcca atgggtaaac cgacagcaaa aatcagcggc atattacgga
    26881 acaccgtcca accgccctct tcaataatgt gtacgatttg cgcgaataaa ctgttcggat
    26941 cggtcagtga ttccccgaca aacatcgggt tttgcagcaa gatggcaaga cccaccacaa
    27001 tcccggcgaa gggaaacagc agcactggcg tgaacatcgc gccgccaaag cgttgaattt
    27061 gactgagcat ttccacatcc tcttttctca attctgtaag taggaagcct gattggttat
    27121 gcggcaggcc tggagcgagg ataggaagat gcgatcggca gaacatgacg acccaatggg
    27181 attcgtgatc gctttcatgc ttttcgttgg gcggtagaga tctacgtttt cgcgaaaaag
    27241 tagacatctg tgagcacagg tacacagcat cgaacacatc tttaaaaaaa agatgttttt
    27301 tcaatcgatt aagcagaact tgtgggcgca ttacccgtgc ttgcaggcaa aaaagagatc
    27361 tagagatgat ctacaaaagc attgcggagc ggttaagaat tcgacttaat tccgcagatt
    27421 tcacgctaaa tagccttctt cccggtgaaa aaaagttggc ggaagaattt gcggtatcgc
    27481 ggatgaccat ccgtaaagcc attgacctgc tggtagcgtg gggattggtg gtccgccgcc
    27541 acggcagcgg cacttacctg gtgcgcaaag atgtactgca tcaaaccgcc agcctgaccg
    27601 ggctggtgga ggtgttaaaa cggcagggaa aaacggtcac cagccaggtg ctgatttttg
    27661 aaatcatgcc tgcgcctccg gccattgcca gccagttacg gattcaaatc aacgagcaga
    27721 tctacttctc ccgtcgcgtt cgttttgtgg aagggaaacc gctgatgctg gaagacagct
    27781 atatgccggt aaaactgttc cgtaatcttt cgctgcaaca tctggaaggg tcgaagtttg
    27841 aatatattga acaagagtgc gggattttga ttggcggtaa ttatgaaagc ctgacgccag
    27901 tgctcgccga tagactgctg gcgcggcaaa tgaaggtagc ggaacacacg ccactgctgc
    27961 ggatcacctc gttgtcatat agcgagagcg gggagttttt gaattattca gtgatgttca
    28021 gaaatgccag cgaataccag gtggagtacc atttacggcg actccacccg gaaaagagtt
    28081 aaccgatact ccagaagagc accgccagta attggggggt gataatgcgc aggaacatca
    28141 ctaacggata gacagtggcg taagagagcg ccgccgcacc gctggttgga tgaagattat
    28201 tagcaaacgc cagcgccgga ggatcggtca tggaacctgc cagcatcccg cacatggtca
    28261 ggtaattcat tttggctaac atccgcgcca gaatgccaac agtaatcagc ggaacggcgg
    28321 tgatcagggc accataacca atccagctta gcccttcgcc attgaccagg gtattcacaa
    28381 aatctccacc agatttcaga ccaacgaccg agaggaacag cacgatcccc agctcccgca
    28441 gcgcgaggtt ggcacttggc ggcataaacc agtacagctt gccgatactg ccgatacgcc
    28501 cgaggatcaa cgccataatc agcggaccgc ccgccagccc cagtttcaac gcggccggga
    28561 acccaggcac aaagacggga atagaaccta acaatacgcc aagcccgatg ccaataaaca
    28621 ctggcagcat ctgaacctgt tgcagttttt gctgcgcatt ccccagcaca ttggcaacgg
    28681 catcaattgc ggacggacgc cccaccagat tgaggatatc gccgaactgc aggctgatat
    28741 cgccactggc gaccagttcg accccggcac ggttcaggcg cgagataaca acgtcatagc
    28801 gttctttaaa gtgcaggtcg cgaatacgtt ttccgagcac gttttcattg gtgaccacca
    28861 cacgctcgac gcgcaaatca gtgcctttcg tggacagcga agtatcgacc tcctgaccaa
    28921 tcaccagttg cgcattatgt aaatccgctg gctgccccac cagatgcagc aaatcgccca
    28981 gttggataat ggtatctggc gaaggaactt ttagggtttc ttcgcgtttc agacgcgagc
    29041 agataatttt gtcgccgttg agaatcggta catctttaat ggctaaatca tgcaggttag
    29101 ggttctcaac gcgaatattg atagtcttga tcagcgcgcc gccattggtg cgtgaagact
    29161 cgtgctgctg agcttctgtc tcgacattga cgcggaaaat aacccgcaac atccacatgg
    29221 tgaacaaaat cccgcaaatg ccgaatggat acgccatcgc gtaactcatc cccatctgat
    29281 cgaccatttc cattggtgta cccaagtcgc gcaaaatctg ctgccctgcc cccagcgctg
    29341 gcgtattggt aaccgcaccg gagaaaatcc ccagcactac cggcagtggc atatcaaaca
    29401 gtttatgcag gatggcggta accagaccac cgatgatgac gatcagaaca gcaaacaggt
    29461 tgaggcgtaa tccggagacg cgcaatgagg caaagaagcc cggccctacc tgaatcccga
    29521 tagtataaac gaacaggatc aggccaaatt cctgaataac atgcagcata tcgctactta
    29581 atgtcatccc cgcctgagaa acaaaatggc cgacgatgat cccaccaaac agcacgccgc
    29641 caatacctaa tcctatgccg cgaaatttga cattgccgat aaacaaaccg acgactgcca
    29701 ccaaagccag aatactgacc gttaatgcta tatcactcat cgtccatttc tcttgagata
    29761 acatttttaa ttgctacgga ttatggctga atcggtgaag cagatttgcc ttatagcgca
    29821 cattatcacg ggaaagaacg tgccgaaata tcttaaacag tcgaaattta acgcattgtt
    29881 gagtaagtaa gacaaaaaaa gccccaccag aatggcgggg caaagagaat agctagttag
    29941 ctatttaacg cgggacgttc gctgatagcg atacgctgcg ctgcgatggg ttcaggctca
    30001 ttacgaatta aatcaatatg cagtaaaccg tttacgaagg ttgcgccaga gacttccata
    30061 ttttcagcca gcgtaaagct caggctaaat ggctgattca taagcccttg atgcagccat
    30121 tttttctctt cttttggctg ctccggcgtg ccttttacgc tcaggcgcgt accttccagt
    30181 tgaatctcta aatcttcctg acggaaacct gccagcgcaa gggtaatgcg gtagtggtta
    30241 tcgtcgcttt tctcaatgtt gtacggcggg aagctctggc tttcaccggc gttttgcagt
    30301 gcgttggcca gtttgtcaaa accgatccat tgacgcatca gtggggataa atcgaagtta
    30361 cgcatagtca tttctccttc taagaagcga gtaagtacct gcaaatccga agattcgcat
    30421 atgctcccta acggcgagca tggagatgtc aggccgcgcc aggcggcctt agggaattag
    30481 ttgatttcga tacggcgcgg ttttttcgct tccggaatca cgcgttcgag atcgatatac
    30541 agcaaaccat ttaccaggtt agcgccacga acatgaatgt tctcagctaa ctggaatttg
    30601 cgttcaaagt tgcgttcagc gatgccctgg tacagatagg tgcgctcttt ttgttcgtcg
    30661 gcgtgagcac ctttcaccac tagcagatta tcctgggcgg taatttccag ttcgctctca
    30721 gcaaaaccag ccacagcgat agcaatgcgg taatggtttt cgtctaccag ttcaacgtta
    30781 tacggagggt agccgccatt actctggctc tggttgtttt ctaagtggtt aaacaaacgg
    30841 tcaaatccaa tagcagaacg gtaaagcggg gataaatcaa agttacgcat aatcaatagc
    30901 tcctgaaatc agcgagaatg taagaccttc cacaatggac aggtcaggta gccagaacac
    30961 ccatcaggcg cgtcctcatc ggctacgatg taaaaatggg tctggaaatg aacttttcaa
    31021 gccttatcag cggacttttt tttgcagttt atggtctatt gcatagactg agggggcagc
    31081 acgaaacgtt aaagtgcgat agccgccaca gtgcgacgta atgatgatgt tattttcagc
    31141 aagggatcgc tataactcat caggccaaca cccacgatca acagatgaat ttattatgat
    31201 aagaaatgtg ttgttagcgt tcatgatatg cagcggaatg acattactcg gaggatgctc
    31261 cagcgtgatg tcccataccg gagggaaaga aggaacgtat cccggcaccc gcgccagcgc
    31321 aaccatgatt ggcgacgatg aaaccaactg gggcactaag tcgctggcga ttctcgatat
    31381 gccgtttacc gctgtgatgg acacgctttt actgccgtgg gatgtgttcc gcaaagacag
    31441 ctcggtgcgc tcacgcgttg aaaaaagcga agccaacgcg caagccacca atgcggttat
    31501 tccaccggcc agaatgcctg ataactgatt tagcgatcag tttcggtgat ccacagctgg
    31561 ccaccttcca tccacgccag ccattgacca tccggcgaga agacctcggc gtccgcagaa
    31621 ggtggattgg cgtggttttc ggttaaatac tcaacctcgc cgctttgcgc atgggcacaa
    31681 gcaattcgat tatccagcac aaagcccaac cattctcctg acggatgcca gttaaatgca
    31741 gactgaatat ccgttttgtt atgggttaac tggcgcggct cgccgccctg tggcgagata
    31801 agccacagtt gcacaatgcc gttatcatca cgcattaaaa acgcgatttg cgtaccctgc
    31861 ggattacagc gcacccagtg gcgcgggacg ttgactaacc ccggataagc ccgatggtgg
    31921 gtaaaggtta aacgtcgctg cacgacgccg cgcggtggcg cgggcagggt ggtttccgtt
    31981 ccacttaacg gcgcatcacc tgccgctttc cagccagctt catcttgcgg taactcaacg
    32041 ataaacagct ccggcacttt ctcgcccttt ggcgaaagtg tgtcgccaat aaacgccagc
    32101 gcgtgatttc ctacccatcc ttcttcataa gcacgattga tttcatcgct gccaggctgc
    32161 ggcgtgggcg tggttttact caccagcacg caccagtggc taccgctgta ttcacgcgga
    32221 tgctgctttt gtacgttgac cgggccaaac ggcgcagcaa caccgacgtt tcgcaaatcc
    32281 agcgccggat cgagttcatg cattacatgg tcgttatagg taaagctcac cctttcaccg
    32341 ttcgggctaa agacatgcac atggctgccg ccgcgcagcg ctcctggcat atacggcgcg
    32401 gtaatatcca ttgcatcgag attgctcacc ttgccgcctt cagcaatgac tccgcgtcga
    32461 tgatggaaat cgtaatgcca tgtttcatca ggattttccg ggccatggat gaaaacatat
    32521 ttctctgact tcggatgaac ggtcaccacg ccgacgtgtg cgccctgtga cgcgcgatag
    32581 ataacctcga cctcgccggt atggatattc acacgctcaa tggtctcgcc ggtaaacgac
    32641 gccccagaag gacgcacgtc aaataccagc cactggctgt cgggcgtcca ggtattggta
    32701 ttggtgagca ggtgattacg gggagcaaag gttatctgtt tcatggcgcg gtcctgatac
    32761 aaaacgggtc ccgccatcat acttcacaag gacttcactt tcagacaata gtcttttgcc
    32821 atcgtgatga atgtgagtga aaagatggaa catttcgacg tggcgattat tggcctcggc
    32881 ccggcaggat cggcgttggc gcgaaaatta gcaggcaaaa tgcaggtgat cgcgctggat
    32941 aaaaagcacc agtgtggtac tgaaggtttc agcaaacctt gcggcggtct gctggcaccg
    33001 gacgcgcagc gatcttttat tcgcgatgga ctgacgcttc ctgtcgatgt gatcgccaac
    33061 ccgcagattt tcagcgtcaa aactgtcgac gtcgccgcat cgctcacgcg taactaccag
    33121 cgaagctata tcaatattaa tcgccatgct ttcgacttgt ggatgaaatc gctgatcccc
    33181 gccagcgttg agatttatca cgacagcctg tgtcgaaaaa tctggcgtga ggatgataaa
    33241 tggcatgtca tttttcgtgc agacggctgg gagcaacata ttactgcccg ctatctggtc
    33301 ggtgccgatg gcgcaaactc gatggtgcgg cgacatctct acccggatca tcaaatccgt
    33361 aaatatgtcg ctatccagca gtggttcgca gagaaacatc cggtgccgtt ctactcctgc
    33421 atctttgata attcgataac taactgttat tcatggagta tcagcaaaga cggttatttt
    33481 atctttggcg gtgcttatcc aatgaaagac ggtcagacgc gtttcacgac gctgaaagag
    33541 aaaatgagcg cctttcagtt ccagtttggt aaggcggtga aaagcgaaaa atgcacggtg
    33601 ctgtttccct cacgctggca ggattttgtc tgcggtaagg acaacgcctt tctgattggc
    33661 gaagcggcag gatttatcag cgccagttcg ctggagggga ttagctatgc gctggatagc
    33721 gcagacattc tgcgttcggt gttactgaag cagccagaga agctcaatac cgcttactgg
    33781 cgcgccaccc gcaaactgcg tttaaaactc ttcggcaaga tagtaaaaag ccgatgcctg
    33841 accgcaccgg ctttaagaaa gcggattatg cgcagtggta tggcgcatat tccacagttg
    33901 aaagattagc caacgcgctt cacatcgccc accagcagga tgtaagagag cgcgccaatc
    33961 aacgcgacgg cggagatata aaccagtgca ggtgcgaaac cgtaaccctg cgccaggtac
    34021 cccaccacca gcggaacggt gatgccgccc agaccaccgg cgaagttaaa cacgccgccg
    34081 gttaaaccaa tcaggcgcat cggtgccaga gaagagacca gcgaccaggt aatcgaagca
    34141 aaaccgttgc cgaagaatgc cagcgccatc aggcacataa tcatcatcgg atcgttggtg
    34201 tagttagcgc ccataatgca ggtggagatc agcaagccgc agataatcgg cgttttacgc
    34261 gcaaagccca gtgaaaagcc cttacgtacc agcagatccg cgacccagcc agagagcagg
    34321 acgccgacaa acgccgcgag gaatggcacc gtggtcataa agcctgcttt cagcgccgtg
    34381 attccttttt cctgggttaa atagttcggg aaccaggtta agaaaaacca cagtgtagaa
    34441 gccaccgcaa attgcccaag ataaacgccg atcagtttac gatggaacac cagtttccag
    34501 tctttggctg ttaacggctg acgcgcctct ttcttcaccg gcgcatcacc atccaccaga
    34561 ccgccgccat cacgaatgta atccagttca gctttgctga tacctttggt caggcgcggc
    34621 ggctgataaa ccttaaacca aatcagcgac cagataatgc cgataccacc agtgacaatg
    34681 aacacccagt gccagctcaa catctcctga atccagatca gcagcggagt caaaaacgcc
    34741 agaccgacaa actgaccaga cgtataaaaa ccaacggcgg aagcgcgttc atgttccggg
    34801 aaccagctgg tcaccatccg gttattggtc gggaaagcgg gcgcttcgaa aataccggtt
    34861 atcgcacgca gaccaattaa tgacattaat cccgtggcaa agccctggaa taaagtcgcc
    34921 actgaccaac caaatatcgc aataaaataa gtcacgcgag aacctacgcg atctaaaaac
    34981 caaccgccgg ggatctggca tagcgtataa agccaggcga aggccgaaaa tacatagccc
    35041 atttccgctt tcgtaatgcc gaactcttcc tgaatatggg cggaagccac ggccaggtta
    35101 gcgcggtcaa cataacaaat gaccaccgta ataaagatca tcaccagcgt cagataacgc
    35161 cgacgccccg gctttgctgc attaacggga atatccatag cgagctttct ccagattttg
    35221 ggcatagcga agccgctcac catgccctgt aatttacaga gggttatatt tttgtattgc
    35281 tgttttagtg cccgatgagg ggcttacgtg gcaggaatta ccactctgct acgctgttat
    35341 cttcatgacg ccagagcgga ttacgccagt ccggggcatt tttactgaac tcaataactt
    35401 tagcttcgtc gatttccaca cctaagcccg gtttcgttaa cggtttaaag aagccgccaa
    35461 ccatactgaa gtcttctttg tttttcacaa agtcgagtaa ctccgcgcct ttgttgtaat
    35521 gaatgcccat actttgttcc tgaagtaccg cgttatagga aacaaagtcg atatgcaggc
    35581 aagccgccag tgcaatcggt ccgagcggac agtgcggcgc aagggtcaca tcataggctt
    35641 ctgccattcc agcaattttg tagcattcgg taataccgcc tgcatgggag agatccggtt
    35701 gcagaatcga aataccgcct gcctccagca cgcgtttaaa atcgaaacgt gagaacatgc
    35761 gctcacctgc cgccagtgga atatgcgttt gtgccgccaa tttcgggtag tattcggctt
    35821 gttccgccag caccggctct tcaataaaca gcgggcgata cggctccagt tctttaatca
    35881 gcactttcgc cattggcgcg ctgacgcgac cgtggaaatc aagaccaaac tcaatctgat
    35941 tgccaaaagc ttcacgaatt tgtgccacgg tgttgactgc cgcatctacc gcgcgggagt
    36001 tatcaattag ccccagttct tcacaaccgt tcagtttgaa ggtatcgaag ccgatttcgc
    36061 gcagggtttt aatgccgtcg ataacatccg ccggacgatc gccgccgacc caactgtagg
    36121 ctttaatttt gtcgcgaacc aggccgccca tcagttgcca gaccggcgca ttcagcactt
    36181 tgcctttgat atcccataac gcctggtcga tcccggcaat ggcgctcatc aggattggac
    36241 cgccacgata aaatccggcg cgatacatca cttgccataa gtcattgatg cgcgaaggat
    36301 cctgaccaat caaatagtca cccagctcgt gaactgccgc ttccaccgta cgggcgcggc
    36361 cttcaatcac gggctcgccc caaccgacca cgccttcatc ggtttcaatt ttcaggaaca
    36421 tccagcgggg aggtaaacga tacgtggtaa ttttggtgat tttcattgca ctgcctctcg
    36481 atacgcctta acaaatgctg ctgcctgctg cgcggtgcgt tctacggact gcccggcgcg
    36541 atagagatcg ctgcctaagc ccgccccagc acaacctgcg tctatccact gcgccaggtt
    36601 ttctggcgtc acgccgccaa cggcaaagac tgcgatgtcc gatggcaata ccgcttttaa
    36661 cgctttgatg tattgcggac caaaagccga tgacggaaat attttcagcg cctgcgcgcc
    36721 cgcttcgagc gcggtaaagg cttcggtcgc cgtcgcgcag ccggggcaga cggtcatgcc
    36781 gtagcccacc gcacggcgga tcacttcact atggatattg ggcgtaacga tgagctgaca
    36841 acccatcctg gcgagcgcat cgacctgttc aggtttcagt accgtacctg cgccaatcaa
    36901 cgccttgtcg ccatacgcat caacgatggc gggaatgctt tgctcccatt gtggggaatt
    36961 cagcgggatt tcaaccgcgt cgaacccggc gtcaatcacc gcgccaacat gcgccagcgc
    37021 ctcgtcgggc ttaatgccgc gcaaaatggc gatcagcgga agtttagttt gccactgcat
    37081 gagcgatgct ccttatacca gcctgaaatg ccgtgtcgcc cgccaccgcc gtcacgtcgc
    37141 aacccatcgc ctgaaaggct tgctggtagc gcgcggtcag cgatgttccg gcgacaaggg
    37201 tgatggcgtg ttgatgggtc acatagtcgc gcatactggc aacctctgcg ccaatcaaca
    37261 aaccagagag aaattcgctg acctgttcgc ggggaagtgt tcccagcaca tgcgaggcgc
    37321 gaacttcaaa aagctgcggc aatatggcgg gcgcattaag gccgcgctca aggccagccg
    37381 cgaaggcatc ggcagagttt tcctgcggcg gcaaacctgc gccaatcaat gagtgattta
    37441 acagtaaatg atgtaattca ccggtcatca cggtgcgaaa atcgttgatt tgctggctat
    37501 cggcctgcac ccatttgcaa tgcgtaccgg gcataacata aagagaggaa ggagccagag
    37561 ttcgcgcgcc gatcaattgt gtttcttcgc cgcgcatcac attgtggtta tcgtcatgag
    37621 agacgcataa tccgggaata atccagatat tgtcgccaac agacgttaat tgttcgccaa
    37681 tagacgaaaa acgggcagga acagataaat acggagcaac tttccagccg acattgctgc
    37741 cgaccattcc tgccattacc actggcgtat tctcttcacg ccagtcggtc gtgacttctg
    37801 ctaacaccgc agccggagat tttccgttca ggcgcgtgac gcctgcttct gattgcctgc
    37861 tctccaggca gtggtcgccc tgataaagcc aggcgcgaag attggtcgat ccccagtcaa
    37921 ttgcgatgta gcgagctgtc atgtgatttc ctttaacctt cgtgtcgagc tggcgatcat
    37981 ggtaagcgcc gcctgctctg ccgcatcgcc gtcctgatgc cgtattgcat cgaacagcgc
    38041 cttatgttcc tggagcgttt gcggcatgtt ggcctcatcg cccatccagg ttcgttcaaa
    38101 aaccgcccgc tgcaacgaac tgatcgcaat gctaagttgc tgtaacaccg ggttatgtac
    38161 cgactgcagc accgcctcgt ggtagcgaat atccgcttcg ttaaacgctt cgcggtcctg
    38221 attgttggca atcatctcgt tcagcgccga ttcaatctgc gccagatcgc tggaagtcgc
    38281 gcgctctgct gcccaacggg caatcgccgg ttccaccaga tttcgcactt cgctcatggc
    38341 actgataagc cgtgggtcgt agtcgttttc cagcacccat tgcagtacgt cagtgtcgag
    38401 gtaattccac tggttacgcg gtgccacaaa cgcgccgcga taacgtttca tttcaatcag
    38461 ccgtttcgcc atcaacgaac ggaacacctc acggatgatg ttgcgcgagg ttgcaaactc
    38521 ttcacagagc tccgcctcag ccggaagtgg cgagccaggt acgtatttgc cgtgaacgat
    38581 ctgtttaccc agcgtaatga caatgcgatc ggttttattg agagtcatgg agagtccttg
    38641 tgctctgtat gttcttctct actttacccc gatcgatgca taacgcggca actttgtagt
    38701 accagcgtga tgacgttcgc gtttgccgtg tgcgtaatgt agtacaaact tatattgttg
    38761 tactacaatt tagatcacaa aaagaacaat gcataaaaaa atgacatgcg tcgggcagaa
    38821 atctgaaaag ggatatcagg cgctaaacag gagggaaaga agagtatgct ttcaacggct
    38881 tagctactcg tttaaaggat taatcatgaa gttgaatttt aagggatttt ttaaggctgc
    38941 cggtttattc ccactggcgc tgatgctttc aggctgtatc tcgtatgctc tggtttccca
    39001 taccgcaaag ggtagttcag gaaagtatca atcgcagtca gacaccatca ctgggctatc
    39061 gcaggcaaaa gatagtaatg gaacaaaagg ctatgttttt gtaggggaat cgctggatta
    39121 ccttatcact gatggtgccg atgacatcgt taagatgctc aatgatccag cacttaaccg
    39181 gcacaatatt caggttgccg atgacgcaag atttgtttta aatgcgggga aaaagaaatt
    39241 taccggcaca atatcgcttt actaccactg gaataacgaa gaagaaaagg cactggcaac
    39301 gcattatggt tttgcctgtg gtgttcaaca ctgtaccagg tcactggaaa acctaaaagg
    39361 cacaatccat gagaaaaata aaaacatgga ttactcaaag gtgatggcgt tctatcatcc
    39421 gtttaaagtg cgattttatg aatactattc acccagaggc attccggatg gtgtttccgc
    39481 agcattactg ccagtgactg ttacgctgga catcattact gcaccgctgc aatttctggt
    39541 tgtatatgca gtaaaccaat aatcagtaag cgggcaaacg cgtttatgct gtttgcccgt
    39601 ccactgatta attcagcaca tacttctcaa tagcaaacgc cacgccatct tcaaggttgg
    39661 atttggtgac aaagttcgcc acttctttca ccgaaggaat agcgttatcc atcgccacac
    39721 cgacgcctgc atattcaatc attgcgatgt cgttttcctg atcgccaatc gccatgattt
    39781 cttccggttt aatacctaac acgtcggcca gtgatttcac ccccgtacct ttgttaacgc
    39841 gtttatcgag gatttcgagg aagtacggcg cacttttcag cacggtatat ttctctttca
    39901 cttcctgcgg aatacgcgcg atagcctggt cgaggatggc gggttcatca atcatcatca
    39961 ctttcaggaa ctgggtattg gggtccattt tctccgcttc gcagaacacc agcggaattg
    40021 tggcaacgaa ggattcatgc accgtgtagt agctgatatc acggttggcg gtgtacagcg
    40081 tggtgcggtc cagggcgtgg aaatgagaac cgacttcgcg agagagtttt tccaggaaac
    40141 ggtagtcgtc atagctgaga gcggtttgcg ccacggtgct accatcagcg gccttctgta
    40201 ccagcgcgcc gttataagta atgcagtagt cgcccggcag ttccatatgc agctctttca
    40261 ggtaattgtg aacacctgca taagggcgac ccgtcgttag cacgacattc acgccacggg
    40321 cgcgagctgc ggcaatcgca tttttaacgg cgggtgaaat ggtgtgatcg ggtagcagaa
    40381 gggtgccatc catatcgata gcaatgagtt tgatagccat gagttcccca ggtagattag
    40441 ttcctgaccc atgctaacgc gattccgctc aaaaatcagt acaacacccg agggaaaagg
    40501 gggatgcaac gcgggttgca tgctccccat ttgcttagcg gaagagtttc cctttcagca
    40561 gttccatgcc tgcggaaagc agatcgttat tggcttgtgg agacacttca ccttgcggtg
    40621 agagcgcatc aataatcttc ggcaattgtt ctgccagtaa actggaagct gtactggtat
    40681 ccacgccaag tttttgtcca agatcggaca ccgcatttgt gccgagcgcc gattccagtt
    40741 gctcgccact aaccgattga ttgccctgtt gattactcag ccaggttgag agaatggccc
    40801 ctaagccgcc actttgcagt ttttccagca gcacctgaat gccgccctgc tcctcaaccc
    40861 aacttaaaat agcctgatat ttccccgcat cgcctttcag aaaggcaccg acaacttcat
    40921 caaaaagccc catgataatc acctgtaaag cgttacgtgt tgacccaaaa agtatagatt
    40981 tgcgggatga taattgcgga ttgcagaaat aaaaagggcg gagatgatct ccgccctttt
    41041 cttatagctt cttgccggat gcggcgtgaa cgccttatcc ggcctacaaa atcatgaaaa
    41101 ttcaatacat tgcaagattt tcgtaggcct gataagcgta gcgcatcagg cacgctcgca
    41161 tggttagcgc cattaaatat cgatattcgc cgctttcagg gcgttctctt caataaacgc
    41221 acggcgcggt tcaacggcgt cgcccatcag cgtggtgaac aactggtcag cggcaatcgc
    41281 atccttaacg gtaacgcgca gcatacgacg gctttccggg tccatggtgg tttcccacag
    41341 ctgttccggg ttcatctcgc ccagaccttt ataacgctgg atggagaggc cgcgacggga
    41401 ctctttcacc agccagtcca gcgcctgctc gaagctggct accggctgac gacgctcgcc
    41461 acgttcgata aacgcatctt cttccagcaa gccacgcagt ttctcaccca gcgtgcagat
    41521 acgacgatat tcgccaccgg tgataaactc gtgatccagc ggatagtcag tatccacacc
    41581 gtgggtacgc acgcgaacaa tcggctcgaa caggttttgt tcggcattgg tgtggacatc
    41641 aaacttccac tggctgccgt gctgttcttt gtcgttcagt tcgctgacca gcgcgttcac
    41701 ccagcgggta acggtctgct catcagagag gtcggcttcc gtcagcgtcg gctgatagat
    41761 aagctctttc agcattgctt tcggataacg acgctccatg cggttgatca ttttctgcgt
    41821 cgcgttgtac tcagacacca atttctctaa cgcttcgcca gccaatgccg gtgcgctggc
    41881 gttggtgtgc agcgttgcgc catccagcgc gatagagatc tggtactgat ccatcgcttc
    41941 gtcgtcttta atgtactgtt cctgcttgcc tttcttcact ttgtacagcg gcggctgagc
    42001 gatgtagacg tgaccgcgtt caacgatttc cggcatctga cgatagaaga aggtcaacag
    42061 cagcgtacga atgtgcgagc cgtcgacgtc cgcatcggtc atgatgatga tgctgtgata
    42121 acgcagtttg tccgggttgt actcgtcacg accgatacca cagccaagcg cggtgataag
    42181 cgtcgccact tcctgagaag agagcatctt atcgaagcgc gctttctcga cgttgaggat
    42241 tttacccttc agcggcagaa tcgcctggtt cttgcggtta cgcccctgct tcgcagagcc
    42301 gcccgcggag tccccttcca ccaggtacag ttcggaaagc gccggatcgc gttcctggca
    42361 gtctgccagt ttgcccggca ggcccgctaa gtcgagcgca cctttacggc gggtcatttc
    42421 acgcgcgcga cgcgccgctt cacgggcacg ggcagcatcg ataattttgc caaccacgat
    42481 tttcgcgtcg gttgggtttt ccagcaggta ttctgccagc agttcgttca tctgctgttc
    42541 aaccgctgat ttcacctcag aagaaaccag tttgtctttg gtctgggagg agaatttcgg
    42601 gtccggcact ttcacggaaa cgaccgcaat caggccttca cgcgcatcgt caccggtggc
    42661 gctaactttg gcttttttgc tgtagccttc tttgtccatg taggcgttca gggtacgggt
    42721 catcgccgca cggaagcctg ccaggtgagt accgccgtca cgctgcggaa tgttgttggt
    42781 aaagcagtag atgttttcct ggaagccatc gttccactgc aacgccactt cgacgccaat
    42841 accgtctttt tcagtggaga agtagaagat attcgggtgg atcggcgttt tgttcttgtt
    42901 cagatattca acgaacgcct tgatgccgcc ttcatagtgg aagtggtctt ctttgccgtc
    42961 gcgcttgtcg cgcagacgaa tggaaacgcc ggagttgagg aacgacaact cacgcagacg
    43021 tttcgccaga atttcatatt cgaactcggt cacattggtg aaagtttcga ggctaggcca
    43081 gaaacgcacc atggtgccgg ttttttcagt ctcgccggta accgccagcg gggcctgcgg
    43141 tacaccgtgt tcgtagatct gacggtgaat tttaccctcg cgctggataa ccagctccag
    43201 tttttgcgac agggcgttta ctaccgaaac accaacgccg tgcagaccgc cggacacttt
    43261 ataggagtta tcgtcgaatt taccgcctgc gtgcagaacg gtcatgatca cttccgccgc
    43321 cgatacgccc tcttccgggt gaataccggt cggaatgccg cgcccgtcat cctgtacaga
    43381 gacagagttg tcggcgtgaa tggtgacgat aatttcttta cagtgacccg cgagcgcttc
    43441 gtcgatagcg ttatctacca cctcgaatac catgtggtgc agaccggtgc cgtcatccgt
    43501 gtcgccgata tacatacccg ggcgcttacg caccgcatcc agccctttca ggactttgat
    43561 actggaggag tcataagaat tcgacatcaa cgtttctcgc tcatttatac ttgggttaat
    43621 ccgttatttt acccttttcc acggtaaaca tcttcgaatt ttcgtccgac atgtctataa
    43681 cgtgttcagc actgatcgcg ctgacaaaga cctgtgattg cgtcgctttt aagcggctgg
    43741 caagcaaccc acgacgctca tcatcaagct cagaggcaaa atcatctatc aggtagagac
    43801 accgccgccc gctttcacgg gtgaggaact ctccttgcgc cagacgtaag gcgcacatca
    43861 acagcttaag ctgcccacgc gataaggtat cttccaccgg cgcaccgtcg gcgcgaatgc
    43921 gtaagtccgc tttatgcggg ccatgcgcgg tgtaggttag ctggcgatcg cgttcaaaat
    43981 tacgttccag cacctcagca tattctgtct ctttctccca gccgcgctgg aaagagaaag
    44041 tcagagaaaa ctcagggaga aattgcttac aggtatcggc catatcggcc gcgataccgg
    44101 cgctatactc cgcgcgccag gtgctgattt gctccgccag cgggatcagt tctttatccc
    44161 acgggcgtag ctgttcgtaa cgtgtcacct ggcgcagcgc cgcattgcgc tgcttgagca
    44221 atcgcttgag attgctccag gcggtgaaaa atccgggttc gttgtgaaag catccccagt
    44281 cgaggaatgc tcttctgtat ttggggccgc cgttgagtaa agtaaaccct tctggcgtta
    44341 tcagctgcat tggcatcagg tgcgccagtt ccgcgacttt atgcccgtca gtaccgtcga
    44401 tgcggacttt gctgtcgccc tgtttgtcct tggttaagcc aatcgccgtc tcgcgctctt
    44461 cgccctgtaa tcgcccatgg agaacaaatg cctcctgctc atggcgaatg acgcgaccaa
    44521 tctgcaaact gcgaaacgcc cgaccatggc cgagcgtata gatggcttcc agcacgctgg
    44581 ttttgccact gccgttggca cctaccagaa agttaaagcc gggagataaa gcgagatccg
    44641 cggtttcaat gttgcggaaa tcgcggatca acaagcgggt gagggacatt acagtctcat
    44701 tggcatgaca acataggcag ccgactgtga tgcggcatct tcaatctgca cgctggaaac
    44761 cgaatcggtc agcatcatgc ggacgttttc gcatttcagc gcgttcagaa catccagcac
    44821 atagctgacg ttgaagccga tttccatctc cgcaccgcta taggtaacgt cgaggatctc
    44881 ttccgcttct tcctgttccg ggttgttggc ggtgattttc agctggtttt cgctgacata
    44941 caggcgcacg ccgcggaatt tctcgttaga gagaattgcc gcacgggcaa acgcctgctt
    45001 gagcagatcg cagccagctt ccagatgttt gtccggattc ttcggcagaa cgcggcggta
    45061 atccgggaag cgaccatcca ccagtttgga ggtgaagata aagtcgccaa cgtgggcgcg
    45121 aatattgttg ctgccaatct gcacgcgcag cggattgtcg ccgccgtcga gcatacgcat
    45181 cagttcaatc acgcctttac gcggtacgat caccgaatgg cttggcaaag attgaccaat
    45241 tggcattgaa cagaccgcca gacggtggcc gtcggttgcc acggtgcgca gttcttcacc
    45301 ttcggtttca aacagcatac catttaagta atagcgaacg tcctgatgcg ccatcgaaaa
    45361 ctgggtcgct tcaatcagac gcttcatcgt tgcctgcggc agggtaaatt cgacttcact
    45421 ctgccagtca tcgaggttcg ggaaatccgc cgctggcagg gtagacagcg aaaaacggct
    45481 acgcccggag cgtaccagca tccgttcacc ttccagctgc acggcaattt ccgcgccttc
    45541 aggcagacca cggcagatat caaagaattt gcgcgccgga acggtcgtcg ctcctggctc
    45601 gtgtggctga accagcgcaa cacgtgccac catttccatc tcgagatcag taccggtcag
    45661 cgacaacgta ccgtcagcaa cctgtaacag cagattaccg agaatcggta gcgtaggacg
    45721 accacctaac ggaccgctca cctgttgtag cggttttaat aaatgctcac gttctacggt
    45781 aaatttcata ggtttacgat gacaatgttc tgattaaatt tgaaaaatct tctttgatat
    45841 cgtggctctc ttcacgcaac tgctcgatct tacggcaggc atgaagcacc gtcgtgtggt
    45901 cacgaccacc aaacgcatcg ccaatctccg gcagactgtg gttagtcagc tctttcgcca
    45961 gcgccatcgc catctggcgc ggacgcgcca ccgagcggga tcgacgcttg gaaaggagat
    46021 ccgcgacttt gatcttgtag tactccgcca ccgtcttctg aatattgtcg atggtgacca
    46081 gtttttcctg caatgccagc aagtcgcgca gcgcctcacg cacgaagtcg atggtgatcg
    46141 cccgtccggt aaagttggca ttggcaatga cgcggttcag cgccccttcc agctcacgta
    46201 cgttagatcg tagacgcttg gcgataaaga acgccacttc gcccggcaaa cgaatgtcgt
    46261 tttcgtcggc ctttttcatc aggatcgcca cacgggtttc cagctctggc ggttcgattg
    46321 ccacagtcag tccccaaccg aagcgggatt tcaaacgatc ctcaacgccg ttgatctctt
    46381 tcggatagcg atccgaggtg agaatgatct gttgattacc ttccagcagg gcgttgaagg
    46441 tatggaaaaa ctcttcctga gatcgttctt tattagcaaa aaactgaata tcgtcgatca
    46501 gcagtgcatc tacggaacgg tagtagcgtt taaactcttc gatcgcgttg ttttgcaggg
    46561 ctttaaccat gtcctgaaca aagcgctcgg agtgcatata aaccactttg gcattcggct
    46621 tgcgcgccat aatgccgtta cccaccgcat gcagcaggtg agttttaccc agacccgtgc
    46681 cgccataaag gaacaacggg ttataggcac cgccagggtt atccgccacc tggcgagccg
    46741 ccgcgcgcgc cagttggtta gatttacctt caacgaagtt atcaaacgtg tgtttgacgt
    46801 ttacgttaga acgataggtc ggttctgccg gggccgggat gttatcccaa cctgagcgcg
    46861 tagaaggcgc agcacgttgc ggctgcgttt gcgccacctg tgcaggggcc gcgacgttgc
    46921 tcgtcactgc cgcttgtggc gtttgcgtca ccggtttggt gccgacttca aaacgcagct
    46981 gtggggcatc cgctccgcag aaactggtta gcagtccatt gatattatta aggtacttgt
    47041 cccgtaccca atcgaggaca aaacggtttg gcgcgtacag ggccagcgtg ttatcgctca
    47101 gttccgcctg caatgggcgt atccacatac tgaattctgt ggctggtaac tcatcctgca
    47161 atcgggcaag acactgctgc caaagcgaaa gtgacacggc ggactccact cgaacaaaag
    47221 tcgataatga ctaaggctga aacattcatg attgttgacg tacgtcgaaa agaccctgct
    47281 tgagggtgac gcacgaaccg ctgtctgcgg ttatatgccc gatcaagatc ctgcaaaacg
    47341 atcgggaccg cggatcatag cctaaactgc gcaagagatc ttctgtttct cacagatttt
    47401 cccgatttat ccacaggact ttccagacct cgctaagtgt aaacgatcct gccgcgaggc
    47461 gggcacgatt tacgccgcat attggaaaat ttaatgacca tagacaaaaa ttggcttaat
    47521 cgatctaata aagatccagg acgatccttg cgctttaccc atcagcccgt ataatcctcc
    47581 acccggcgcg ccatgctggt tttcactggt gtgaggtcgt acattttccc tgcgaaaagg
    47641 tgcggaaaag cgcggtaaat aaggaaagag aattgactcc ggagtgtaca attattacaa
    47701 tccggcctct ttaatcaccc atggcttcgg tgtccatcgt ttcatttttc ggcggatatc
    47761 caataaagcc attgaattta ttcaagttta ggtagaaatc gccatgaaac gcacttttca
    47821 accgtctgta ctgaagcgca accgttctca cggcttccgt gctcgtatgg ctactaaaaa
    47881 tggtcgtcag gttctggcac gtcgtcgtgc taaaggccgc gctcgtctga ccgtttctaa
    47941 gtaataaagc taacccctga gtggttaagc tcgcatttcc cagggagtta cgcttgttaa
    48001 ctcccagtca attcacattc gtcttccagc agccacaacg ggctggcacg ccgcaaatta
    48061 ccattctcgg ccgcctgaat tcgctggggc atccccgtat cggtcttaca gtcgccaaga
    48121 aaaacgttcg acgcgcccat gaacgcaatc ggattaaacg tctgacgcgt gaaagcttcc
    48181 gtctgcgcca acatgaactc ccggctatgg atttcgtggt ggtggcgaaa aaaggggttg
    48241 ccgacctcga taaccgtgct ctctcggaag cgttggaaaa attatggcgc cgccactgtc
    48301 gcctggctcg cgggtcctga tagccctcat tcgggtctat caacgcctga ttagtccgct
    48361 actcgggccg cattgtcgtt tcactccaac ctgttcaagc tacggaattg aggcattgcg
    48421 caggtttgga gtgataaaag gcagttggtt gacggtgaaa cgcgtattaa aatgccaccc
    48481 tttacaccct ggtggtgacg atcccgtccc gcccggacca tttgatacca gagaacacta
    48541 acgatggatt cgcaacgcaa tcttttagtc atcgctttgc tgttcgtgtc tttcatgatc
    48601 tggcaagcct gggagcagga taaaaacccg caacctcagg cccaacagac cacgcagaca
    48661 acgaccaccg cagcgggtag cgccgccgac cagggcgtac cggccagtgg ccaggggaaa
    48721 ctgatctcgg ttaagaccga cgtgcttgat ctgaccatca acacccgtgg tggtgatgtt
    48781 gagcaagctc tgctgcctgc ttacccgaaa gagctgaact ctacccagcc gttccagctg
    48841 ctggaaactt caccgcagtt tatttatcag gcacagagcg gtctgaccgg tcgtgatggc
    48901 ccggataacc cggctaacgg cccgcgtccg ctgtataacg ttgaaaaaga cgcttatgtg
    48961 ctggctgaag gtcaaaacga actgcaggtg ccgatgacgt ataccgacgc ggcaggcaac
    49021 acgtttacca aaacgtttgt cctgaaacgt ggtgattacg ctgtcaacgt caactacaac
    49081 gtgcagaacg ctggcgagaa accgctggaa atctccacct ttggtcagtt gaagcaatcc
    49141 atcactctgc caccgcatct cgataccgga agcagcaact tcgcactgca caccttccgt
    49201 ggcgcggcgt actccacgcc tgacgagaag tatgagaaat acaagttcga taccattgcc
    49261 gataacgaaa acctgaacat ctcttcgaaa ggtggttggg tggcgatgct gcaacagtat
    49321 ttcgcgacgg cgtggatccc gcataacgac ggtaccaaca acttctatac cgctaatctg
    49381 ggtaacggca tcgccgctat cggctataaa tctcagccgg tactggttca gcctggtcag
    49441 actggcgcga tgaacagcac cctgtgggtt ggcccggaaa tccaggacaa aatggcagct
    49501 gttgctccgc acctggatct gaccgttgat tacggttggt tgtggttcat ctctcagccg
    49561 ctgttcaaac tgctgaaatg gatccatagc tttgtgggta actggggctt ctccattatc
    49621 atcatcacct ttatcgttcg tggcatcatg tacccgctga ccaaagcgca gtacacctcc
    49681 atggcgaaga tgcgtatgct gcagccgaag attcaggcaa tgcgtgagcg tctgggcgat
    49741 gacaaacagc gtatcagcca ggaaatgatg gcgctgtaca aagctgagaa ggttaacccg
    49801 ctgggcggct gcttcccgct gctgatccag atgccaatct tcctggcgtt gtactacatg
    49861 ctgatgggtt ccgttgaact gcgtcaggca ccgtttgcac tgtggatcca cgacctgtcg
    49921 gcacaggacc cgtactacat cctgccgatc ctgatgggcg taacgatgtt cttcattcag
    49981 aagatgtcgc cgaccacagt gaccgacccg atgcagcaga agatcatgac ctttatgccg
    50041 gtcatcttca ccgtgttctt cctgtggttc ccgtcaggtc tggtgctgta ctatatcgtc
    50101 agcaacctgg taaccattat tcagcagcag ctgatttacc gtggtctgga aaaacgtggc
    50161 ctgcatagcc gcgagaagaa aaaatcctga ttcggtgagt tttcgctaaa ataagggcgg
    50221 tcagttgacc gccttttttc ttttcgtagg gcggataagc accgcgcatc cgccacacaa
    50281 agcaacagga acatcatgag cgataatgac actatcgtag cccaggccac gcctccggga
    50341 cgtggcggtg ttggcatcct gcgcatctcc ggcctcaaag cccgtgaagt tgccgaaacc
    50401 gtgctgggta aactacctaa gccgcgctac gccgattatt tgccatttaa agacgccgac
    50461 ggcagcgtgc tcgatcaggg gattgcgcta tggttccctg gcccgaactc gttcaccggc
    50521 gaagatgtgc ttgaactgca gggtcatggc ggtccggtga tcctcgacct gctgttaaaa
    50581 cgcattctga ccattcccgg cctgcggatt gctcgccctg gtgagttttc cgaacgcgcg
    50641 tttctttacg ataaactcga cttagcccag gctgaggcga ttgccgatct tatcgacgcc
    50701 agctcggaac aggcggcccg ttcggcactt aactcgctgc aaggcgcatt ctccgcacgg
    50761 gttaatcatc tggtagaagc cctcacccac ttgcgcattt acgtcgaagc ggcaattgat
    50821 ttccctgatg aagagatcga tttcctctcc gacggcaaaa ttgaagccca gctcaatgac
    50881 gttattgccg atcttgatgc agtgcgtgct gaagcacgtc agggtagttt gttgcgcgaa
    50941 gggatgaaag tggtgattgc cggacgtcct aacgccggta aatcgagcct gttaaacgcg
    51001 ctggctggcc gtgaagcagc aatcgtaacc gatatcgccg gaactacgcg tgacgtgttg
    51061 cgtgagcata tccacattga cggaatgccg ctgcatatca tcgacaccgc cgggctacgt
    51121 gaagccagtg acgaagtgga acgtattggg atcgagcgcg cgtggcagga aattgaacag
    51181 gccgaccggg tgctgtttat ggtcgatggc accacaacag acgccgtgga tccggcagag
    51241 atctggccgg aatttatcgc ccgtctgcca gcgaaactgc cgatcaccgt ggtgcgcaat
    51301 aaagccgata tcaccggcga aacgctggga atgagtgaag tgaacggtca cgcgttaatt
    51361 cgtctctcgg caaggactgg tgaaggcgtg gacgtgctgc gtaaccatct caaacagagc
    51421 atgggctttg acaccaacat ggaaggcggt ttcctggcgc gccgtcgcca cttacaggcg
    51481 ctggaacagg cagcggagca tttgcagcaa ggtaaagcgc aactgttagg tgcctgggcg
    51541 ggtgaactgc tggcggaaga gttgcgcctg gcacagcaga acttaagcga aatcaccggg
    51601 gaatttactt cagacgacct gctggggcgg attttctcca gcttctgtat tggtaagtaa
    51661 ccgcgcttac gaagccgtat tctgactgtc agatgcggct tcgcttcatt gttagcactc
    51721 ctgttattcc tcaacccttt ttttaaacat taaaattctt acgtaattta taatcttaaa
    51781 aaaaagcatt taatattgct ccccgaacga ttgtgattcg attcacattt aaacaatttt
    51841 agaatagaca aaattcttga gtattatact gtagcctcgt gtctttcgag gataagtgca
    51901 ttatgaatat cttacatata tgtgtgacct caaaatggtt caatatcgac aataagattg
    51961 tcgatcaccg cccttaattt acccttctgt agccatcacc agagccaaac cgattagatt
    52021 caatgtgaac gattagtttg ctatatctta attttgcctt ttgcaaaggc catctctcgt
    52081 ttatttactt attttagtaa atgatggtgc ttgtatatat acatggcgaa ttaatggata
    52141 ttgcagatgt aatattcaca gggatcactg taattaaaat aaatgaagga ttatgtaatg
    52201 gaaaacttta aacatctccc tgaaccgttc cgcattcgtg ttattgagcc agtaaaacgt
    52261 accactcgcg cttatcgtga agaggcaatt attaaatccg gtatgaaccc attcctgctg
    52321 gatagcgaag atgtgtttat cgatttactg accgacagcg gcaccggggc ggtgacgcag
    52381 agtatgcagg ccgcgatgat gcgcggcgac gaagcctaca gcggcagccg cagctactat
    52441 gcgttagccg agtcagtgaa aaatatcttt ggttatcaat acaccattcc gactcaccag
    52501 ggccgtggcg cagagcaaat ttatattccg gtactgatta aaaaacgcga gcaggaaaaa
    52561 ggcctggatc gcagcaaaat ggtggcattc tctaactatt tctttgatac cacgcagggc
    52621 catagccaga ttaacggctg taccgtgcgt aacgtctata tcaaagaagc cttcgatact
    52681 ggcgtgcgtt acgactttaa aggtaacttt gaccttgagg gattagaacg cggtattgaa
    52741 gaagttggcc cgaataacgt gccgtatatc gttgcaacca tcaccagtaa ctctgcaggt
    52801 ggtcagccgg tttcactggc aaacttaaaa gcgatgtaca gcatcgcgaa gaaatacgat
    52861 attccggtgg taatggactc cgcacgcttt gctgaaaacg cctatttcat caagcagcgt
    52921 gaagcagaat acaaagactg gaccatcgag cagatcaccc gcgaaaccta caaatatgcc
    52981 gatatgctgg cgatgtccgc caagaaagat gcgatggtac cgatgggcgg cttgctgtgc
    53041 atgaaagacg acagcttctt tgatgtgtac accgagtgca gaaccctttg cgtggtgcag
    53101 gaagggttcc cgacatatgg cggcctggaa ggcggcgcga tggagcgtct ggcggtaggt
    53161 ctgtatgacg gcatgagtct cgactggctg gcttatcgta tcgcgcaggt acagtatctg
    53221 gtcgatggtc tggaagagat tggcgttgtc tgccagcagg cgggcggtca cgcggcattc
    53281 gttgatgccg gtaaactgct gccgcatatc ccggcagacc agttcccggc acaggcgctg
    53341 gcgtgcgagc tgtataaagt cgccggtatc cgtgcggtag aaattggctc tttcctgtta
    53401 ggccgcgatc cgaaaaccgg taaacaactg ccatgcccgg ctgaactgct gcgtttaacc
    53461 attccgcgcg caacatatac tcaaacacat atggacttca ttattgaagc ctttaaacat
    53521 gtgaaagaga acgcggcgaa tattaaagga ttaaccttta cctacgaacc aaaagtattg
    53581 cgtcacttca ccgcaaaact gaaagaagtt taattaatac tacagagtgg ctataaggat
    53641 gttagccact ctcttaccct acatcctcaa taacaaaaat agccttcctc taaaggtggc
    53701 atcatgactg atcaagctga aaaaaagcac tctgcatttt ggggtgttat ggttatagca
    53761 ggtacagtaa ttggtggagg tatgtttgct ttacctgttg atcttgccgg tgcctggttt
    53821 ttctggggtg cctttatcct tatcattgcc tggttttcaa tgcttcattc cgggttattg
    53881 ttattagaag caaatttaaa ttatcctgtc ggctccagtt ttaacaccat caccaaagat
    53941 ttaatcggta acacctggaa cattatcagc ggtattaccg ttgccttcgt tctctatatc
    54001 ctcacttatg cctatatctc tgctaatggt gcgatcatta gtgaaacgat atcaatgaat
    54061 ttgggctatc acgctaatcc acgtattgtc gggatctgca cagccatttt cgttgccagc
    54121 gtattgtgga taagttcgtt agccgccagt cgcattacct cattgttcct cgggctgaag
    54181 attatctcct ttgtgatcgt gtttggttct ttcttcttcc aggtcgatta ctccatcctg
    54241 cgcgatgcca ccagcaccac tgcgggaacg tcttacttcc cgtatatctt tatggctttg
    54301 ccggtgtgtc tggcgtcatt tggtttccac ggcaatattc ccagcctgat tatttgctat
    54361 ggaaaacgca aagataagtt aatcaaaagc gtggtatttg gttcgctgct ggcgctggtg
    54421 atttatctct tctggctcta ttgcacgatg gggaatattc cgcgcgaaag ctttaaggcg
    54481 ataatctcct caggcggcaa cgttgattcg ctggtgaaat cgttcctcgg caccaaacag
    54541 cacggcatta tcgagttttg cctgctggtg ttctctaact tagctgttgc cagttcgttc
    54601 tttggtgtca cgctggggtt gttcgattat ctggcggacc tgtttaagat tgataactcc
    54661 cacggcgggc gtttcaaaac cgtgctgtta accttcctgc cacctgcgtt gttgtatctg
    54721 atcttcccga acggctttat ttacgggatc ggcggtgccg ggctgtgcgc caccatctgg
    54781 gcggtcatta ttcccgcagt gcttgcaatc aaagctcgca agaagtttcc caatcagatg
    54841 ttcacggtct ggggcggcaa tcttattccg gcgattgtca ttctctttgg tataaccgtg
    54901 attttgtgct ggttcggcaa cgtctttaac gtgttaccta aatttggcta aatccttcaa
    54961 gaagccagcc attcgctggc ttcttgcctc tcaggaaatc acttatgtcc aaatggcaac
    55021 tcgcctgatt ctccttcacc acgtatgctt tgcgtcacct tactatcagg acgctttagc
    55081 ccatgtcccg ctttttaatt tgtagttttg ccctggtttt actttatccc gccgggattg
    55141 atatgtacct cgttggttta ccgcgcatcg ccgccgatct caatgccagc gaagcgcagt
    55201 tgcatattgc gttctccgtt tatctggcgg gcatggcagc tgcgatgtta tttgccggta
    55261 aagtggccga tcgttcaggg agaaagcctg tcgccatccc cggcgcggcg ctatttatta
    55321 tcgcttcggt gttctgttca ctggctgaaa ccagcacgtt atttcttgca gggcgctttc
    55381 tacaggggtt gggcgcaggc tgttgttacg tggtggcgtt cgccattttg cgcgacacgc
    55441 tggatgatcg acgtcgggct aaagtgctgt cgttactcaa cggtattacc tgcatcattc
    55501 cggtgttagc gccggtgctc ggacacctga ttatgcttaa attcccttgg cagagtctgt
    55561 tctggacgat ggcaatgatg ggcatcgcgg tactgatgtt atctttgttt attttaaaag
    55621 aaacgcgccc agcgtccccc gctgcttcgg acaaaccacg agaaaatagc gagtcgctgc
    55681 ttaatcggtt tttcctcagc cgtgttgtta tcaccaccct cagcgtttcg gtgatcctca
    55741 ctttcgtcaa tacatcgccg gtattgctga tggaaatcat gggttttgag cgcggagaat
    55801 acgccaccat tatggcgttg accgctggcg tcagcatgac cgtttcattc tccacgccat
    55861 ttgcgctggg aatttttaag ccacgtacgt tgatgatcac ctcgcaggtg ttattccttg
    55921 cggcggggat cacccttgcc gtttcacctt cccatgcggt ttctctgttt ggtatcacgc
    55981 tgatttgcgc cggtttctcg gtaggttttg gcgtagcgat gagtcaggcg ttaggaccat
    56041 tttcattacg cgcgggcgta gccagctcga ccttaggtat tgcgcaggtt tgcggttcgt
    56101 cactgtggat ttggctggca gcggtggttg gtatcggcgc atggaatatg ctgatcggga
    56161 ttctgattgc ctgtagcata gtgagcctgt tactgattat gttcgtcgcg cctggacgcc
    56221 ccgttgccgc tcatgaagaa atccatcacc acgcttgatc tcaatctgct gctctgtctg
    56281 caactgctga tgcaggagcg cagcgtgacc aaagcggcga agcggatgaa cgtgacccct
    56341 tcagcggtga gtaagtcgct ggcaaagtta agagcgtggt ttgacgaccc gctctttgtg
    56401 aactcaccgc tggggctgtc gcccacaccg ctaatggtca gcatggagca aaatctggcg
    56461 gaatggatgc aaatgagcaa tctgctgctg gataaaccgc accaccagac accgcgcggc
    56521 ctgaagtttg agttggcggc ggaatcgccg ctgatgatga tcatgcttaa tgcgctgtcg
    56581 aaacggatct accaacgtta cccgcaggcg accatcaaac tacgtaactg ggattacgat
    56641 tccttagatg ccattactcg tggtgaagtg gatattggtt tttccggtcg cgaaagccat
    56701 cctcgctcgc gggagctgtt aagctcgcta ccgttagcca ttgattatga agtgctgttt
    56761 agtgatgtac cctgcgtctg gttacgcaaa gatcatccgg cactgcatga aacgtggaat
    56821 ctggacacct ttttacgtta tccgcatatc agcatttgct gggaacagag cgacacctgg
    56881 gcgctggaca atgtgttaca ggagctgggg cgcgaacgca cgattgctat gagcctgccg
    56941 gaatttgagc agtcactgtt tatggcagcg caacccgaca atctgctact ggcgaccgcg
    57001 ccgcgctact gtcagtacta caatcaactc catcaactgc cgttggttgc tcttcctctc
    57061 ccgtttgacg aaagccagca aaaaaagctg gaagttcctt ttaccctgct gtggcataaa
    57121 cggaacagcc ataatccgaa gatcgtctgg ttacgggaaa ccattaaaaa cctttacgcg
    57181 tcgatggcat aaccgaatcg tatgaaacgc gacccaattt cacaataaaa tgtaaaaaag
    57241 ttgtaataag cttgtctgaa tcgaactttt agccgcttta gtctgtccat cattccagta
    57301 aatgattact cttatattca taatggacca ttaagcatgg agcgaaaaat ggcgactcac
    57361 tttgcccgag ggattttaac ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt
    57421 catcaagaag cccgaaacat tccccctcat cgtcaaagcc gttttctggc gtccagaggt
    57481 ttactcgcag aactgatgtt catgctgtat ggcattggcg aattgccgga aatcgtcacc
    57541 ctgccgaaag gtaaaccggt tttcagtgat aaaaatttgc cttcgttttc tatttcctat
    57601 gccgggaata tggttggcgt ggcgttaaca accgaaggtg aatgtggcct cgatatggaa
    57661 ctacagcgtg cgacgcgcgg gtttcatagc ccacacgcgc ccgataacca caccttttcc
    57721 agcaatgaat cgctatggat cagtaaacaa aacgatccta acgaagcgcg ggcgcagctc
    57781 atcacgctgc gccgaagcgt gctaaaacta accggtgatg ttttgaatga cgatccgcgc
    57841 gatctgcagc tgctgcccat tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa
    57901 gcgttatgcg acgcggagga cgtgctagtg tggtccgtgg cggtcacgcc cgcgattgaa
    57961 aagctcagtg tctgggagtt agatggcaaa cacagctgga aaagcctgcc ggatattcac
    58021 agccgcgcca acaatcctac cagccggatg atgcgttttg cccagctctc taccgtgaag
    58081 tctttttcgc caaattgata gacaaccaca ggagtcatta tgtctgaaaa attgcaggtg
    58141 gttacgttac tggggagcct gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg
    58201 ccgaaaattg ctccggcgag catggaagtc aatgcgttac cttccattgc cgacattccc
    58261 ctgtatgacg ctgacgtaca gcaggaagaa ggttttccag caacggttga agctctggcg
    58321 gaacagatcc gtcaggctga cggtgtggtg atcgtcacgc cggaatataa ctactcggta
    58381 ccgggtgggc tgaaaaatgc catcgactgg ctttcccgcc tgccggatca accgctggcc
    58441 ggtaaaccgg tgttgattca gaccagctca atgggcgtga ttggcggcgc gcgctgtcag
    58501 tatcacctgc gccagattct ggttttcctc gatgcaatgg tgatgaacaa gccggaattt
    58561 atgggcggcg tgattcagaa caaagttgat ccgcaaaccg gagaagtgat tgatcagggt
    58621 acgctggacc acctgaccgg gcaattgacc gcatttggtg agtttattca gcgagttaag
    58681 atctaaataa aaaacccgcc agcaatcatg catggcgggt ttttaacgcg ctatcgattt
    58741 tagtgagcgt cgataaagac aatcttcagg ataaacagca gcgcaacgat gattacgcac
    58801 gggcttagat cacgcagacg tccggtaccg attttcatca cgcagtagga gataaagccc
    58861 agcgcgatac cttcggtaat cgagaagctg aacggcatca tcacggcggt aataaacgcc
    58921 ggaacagatt cagtaagatc ctgccagttc acgcgtgcca gactggaagt catcagcacg
    58981 ccaacgtaaa tcagcgcacc agctgcagcg tagcctggca ccatccccgc cagcggcgac
    59041 agaaagataa ccagcaggaa cagcagacca acaaccactg ccgtcagacc ggtacgaccg
    59101 ccaaccgata cgccggaaga ggactcaata taagccgtaa cggaagaagt accgataaac
    59161 gaaccggtca cggaagagat actgtcgaca tacagcgcct gcttcatgcg cgggaattta
    59221 cccttctcat ccgccaggcc tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca
    59281 aacaggttga ccagcatgaa agagaaaatc acccctgcca gcccgaggtt aaacgacccg
    59341 gctaaatcta catgacccac aactgtcatt acgctcggcg gcgcagaaac gatgccattg
    59401 tagtgcacat cacccagcat ccagcccagc agcgtcgtca ccacgataga aaccagcacc
    59461 gctgcgtgaa tgttgcgcga ggccagaata gcaatgatga agaagccgag gatacccaga
    59521 agtacgctgt gagaagtcag attaccgatg ctcaccagcg tttccgggtt agcgacaatc
    59581 acacctgcgt ttttcagccc catcatgcca atgaacagac cgataccgct ggtaataccc
    59641 acacgcagac tcaccggaat gttggcaatc atccagtagc gaacgcggaa aatcgtcagt
    59701 aacagcagac ctatcgcgcc ccagaagatt gcgcccatcc cgacctgcca cggcaagccc
    59761 atcgcctgta caacgacaaa agcgaagaac gcattcaggc ccatagcggg tgccagtgca
    59821 actggcaggt tagcaaacag tcccatcata atactgccga atgcagcgat cagacaggta
    59881 gtgacgaaga cggcgctggt atccatgcca gcaacgccaa gaatttgcgg gttaacaaaa
    59941 acgatgtaaa ccatcgtcag gaaggtggta aaaccggcga tcacttcggt ccgtgccgtc
    60001 gtgccatgtt cgcgcagttt aaacacgcgt tccagcatcc cctggccaga agcctgggtt
    60061 gtgtgttgtt gactcattat ctatttccga acaaggaggg aaaatccgtc gctatcgtat
    60121 accaaaatga gacaatatgg acggctgaag ataacttttt ttcattgtat ttgcttatac
    60181 ggcaacgatt gcgtcatgcg aattggcatg acgaaaacgt taaactaacg ataaaggaaa
    60241 gacatgtccc agatagaagc ggtatttttc gactgcgacg gtacgctggt cgacagtgaa
    60301 gtcatttgct cccgcgcata tgtaacgatg tttcaggaat ttggtattac gctcgatcct
    60361 gaagaggtat tcaaacgttt caaaggtgta aaactgtacg aaattatcga tattgtttcc
    60421 cttgaacatg gtgttacgtt agcgaaaaca gaagctgaac acgtttaccg tgcagaagtc
    60481 gctcggctgt tcgattcaga actggaagcc atcgaagggg ctggagcgct cctgtcagcg
    60541 atcactgcgc caatgtgtgt ggtatctaac ggcccaaata acaaaatgca gcattctatg
    60601 ggcaagctga atatgttgca ctacttcccg gataaactgt tcagcggcta cgatattcag
    60661 cgctggaagc cagacccggc gttaatgttc catgcggcaa aagcgatgaa tgtaaatgta
    60721 gaaaactgca ttctggttga tgactcagtt gccggtgcgc aatctggtat cgacgcaggt
    60781 atggaagtgt tctacttctg cgccgacccg cacaataagc cgatcgttca cccgaaagtc
    60841 accaccttta cccatctttc gcagttaccg gaactgtgga aagcgcgtgg ttgggatatt
    60901 acggcatagt tcttcacact cccttcactt accccgctta aattggcgct caaaggtaag
    60961 taaagggagt ttgatatgtc tgtttcacgt cgggtaatac atcacggact ttattttgcg
    61021 gttttaggac cgttaattgg tgttctgttt cttgtcctct acatattctt cgcaaaagaa
    61081 ccgctggttc ttttgcgaag aatacaggta cttccattgt ttctcttatt gtcgattact
    61141 acgggagtta ttcctgcatt gttaaccggc gtgatggtcg cctgtctgcc ggaaaagatc
    61201 gggtcacaga aaaattatcg ttgtctggct ggtggcatag gtggcgtcgt tatcaccgag
    61261 atctattgtg cagttattgt acatattaag ggcatggctt cctcggagtt gtatgaaaac
    61321 attctttctg gtgacagtct cgttgtccgc atcattcctg cattgctggc aggtgtggtg
    61381 atgagcagaa tcattacccg tctgcccgga ttggatattt catgtcctga aacagactct
    61441 ttaagttaag cgggatactt tatctttggg ctactcaaaa gcagacagga tgttactatg
    61501 actcaaaata tcaggccgtt accccaattc aaatatcatc ccaagccact ggaaacaggc
    61561 gcatttgaac aggataaaac cgtagagtgc gattgctgtg aacaacagac gtcagtttat
    61621 tactcgggtc ccttttattg cgttgatgaa gttgaacatc tctgtccgtg gtgtattgcg
    61681 gacggttctg ctgctgaaaa gtttgcaggt agttttcagg atgatgccag catagaaggt
    61741 gttgaatttg agtatgatga agaggacgaa tttgccggta ttaagaacac atatcctgat
    61801 gaaatgctga aagagttggt tgaacgcacg ccaggttatc atggatggca acaggaattc
    61861 tggcttgcgc attgtggcga tttctgtgct tttatcggct atgtgggctg gaatgatata
    61921 aaagatcgcc tcgatgaatt tgccaacctt gaagaagatt gtgagaattt cggtattaga
    61981 aattctgatc tagctaaatg cctgcaaaag ggtggtgatt gtcagggtta tctcttccgc
    62041 tgtctccact gcggcaagct gagactgtgg ggtgattttt cgtagttatt taaataatga
    62101 gaacaggccg gagcgtaatt catacatccg gccttatttc ttcagcttaa tcgagcgcta
    62161 attctgcggc agcggcttta tcagcgatta ccatcagcga aggatgcagc tgcaacacgg
    62221 aagctggaac atcttcggta accggacctt gcagcacatt tttcagtgcc tgcgctttac
    62281 ctgcgccact gacaattatc agcaagtttt tcgccgccat gatgcttttc ggccccatcg
    62341 tcacgtagct gtcaggcact aacgaaaagt cgccgcccaa ctcaccatgc gccacaatat
    62401 caaccatctc tccctggatc gggaactcca cggtttgctc atggaaatgg gtcgtattcg
    62461 gcaaattgcc acaaaaatga ccatctgcac ctaatcccag caccaccaaa tccaggccgc
    62521 cttcacgcgc cagtttctga tcatgctcgc ggtagttatc aatagtgagc ttctggatat
    62581 tctcttcttt gatccccgca ggggtgaaaa acagattacg cagattggta atcgttacac
    62641 cttctccctc tttgccgcga aagggaattt catcgaaatt atagaaatag cagttatcat
    62701 accagggctt acctttaacc agggtggtga ggtattcata catgcctttg ggcgtgctac
    62761 cggcggtaat tgccaggtta acacgacgcg tcttcgacat ataaccaagc agatggtgtg
    62821 ccgcgacacg gctcatttcc tggtaatctt cggtaatgat taatttcatc ttagtgcctt
    62881 attttattta aataatttct gtacgaaggc tgcataggca ggccgccaga catccatttc
    62941 atggttcagg ccgggatatt cctggtaatc aaacttaatt tttttctgct caagctcagt
    63001 tttcagcccg gcgatatcct tgccggttac gacatcttta tcaccaacga ccacagtaaa
    63061 attacgtagt tgctggttaa tagctgccgg atcgttcagc cgggccgcga caccttcatc
    63121 cggtacggtt gtcgtggtaa caccactgaa tgtggccagc cagccaaagc tttccagatg
    63181 attcattccg gaaaccagcg cctggtaccc gccttgtgaa agccctgcca gcgcgcgacc
    63241 atcggcatct ttacggacat taaaacgctt gctaatcagc gggataatat cgttcatcag
    63301 ttcgcgatct gccgctttag cattcagcgg ataaaagact ttacgtcttt cctgaggcac
    63361 gaaatcttcg ggaataatgc ccttcgcatc ggtttcggta tccgggatca ccaccagcat
    63421 cggtttaatt ttcccttcag ccagcaggtt atccatgatt tgcgggatac gcccctgatc
    63481 gatagcggaa cgtccggtat caccaaaacc gtgatagaaa tagagcactg gcaaaggctc
    63541 gcccatgccg gtgtaacctg gcggggtcca gacatacatc tgacgttcag attgcaatgc
    63601 gttggagtgg taagttatgg cgatcaaatc accatgcgca acagaacgcg tatccagata
    63661 actgcctggc accagaatca tactggagtt aacctggcgc tgaggcttag tcattgcggt
    63721 gcctgtatca atgctgcgta caccatcaac attgaaaaaa tactcgtaca ggttgccttt
    63781 caggataggt gtgcgccacg accagactcc tgcttcgtct ttggtcatcg ggtgaatatt
    63841 gtccggaacc ggaacaccca ccactacaga gacatttttt gccccagggg caaagtagcg
    63901 aaaggtcacg ctgttatcgg cattgacctg agtcacatac tgtttcaccg ggatagtggg
    63961 tgccgggctg gctggcatat ccgctgctat ggcccactga gcagaaatac cgctggcaat
    64021 agccagcgtt aaagcagcaa tttttatatt catcgtaata ttcctttcat taagttccat
    64081 taccaccaga tttcagcctg ggcaccgaca gcgaactggt cgtctttatt gtcttcgaag
    64141 gtgaagccat ccagttcgtt ttccagggct ttgatatacg tggcgtagaa acgaatttcc
    64201 ggacgcgagg tcaacatact ggtattgact ttaaaggtat ggaagagcgt ggttttataa
    64261 ccagactcat taaatttatt actattcgca tctttgtttt gctgggtgaa atagcccagt
    64321 tcaacacctg tctggttata ttggtcccaa atataggccg ggcgaacaac cgcacgaata
    64381 gattcgaaat cagagtgtgc gcctgtttcg tagctataaa tatcgttacc gaaggagtaa
    64441 acaatcgcgt tagccacaat aaaatgatcg ccgatatagg cttcgccctg tgaagtcaga
    64501 cgaactgctg ttccgccggt gtgatcacca taataacgac cattaaaggt ggtaaatgga
    64561 cttgcgccag cataacggcc aaagttactg gcgatagagt tattcgcaac caggaaggag
    64621 aattcgttga agccaccttt atcaaatttc tgcgttaaag atgtgccaaa catccaggta
    64681 tctttccagt cataatagcc gttattatcc tgattatctt tttccgatgc gctttcgttt
    64741 gccgtgacat aacgaccact caccattaag gtcgctttat cccataacgg gatatcttta
    64801 tagcgtaaat caatagtatt ggtattaatc tgctgtttgt tttgcaggct gcgatcgtaa
    64861 tcatcgacat cttcgcgaac cagcgcgata tcaattttac ccggaccgac tttccagttt
    64921 tccagaccta cacccgctgc ggcatcagta cgctgcgttt tccagtccag catctggatt
    64981 tcaattttcg gcgcaccgtg tttacccacc cagaaatcag cctctggcgc aaagggcagg
    65041 aaacctttgg tggtaacgta catatcggag aactgcataa agttctcgcc accggcgtta
    65101 tcgccaaacc agccggtaga gtactgctga ccaacgttac catccatcat cacaacggca
    65161 tcaacccgtt tgccgttttc gttgtagaca cgttgtttta actgcaaatc aaaccagccg
    65221 gagtattcgt taccaaagcg gcccagagaa ccaatcgccc atgatttagg tgaaccatga
    65281 gaggcggtcc cccaaccaga acggtaatag ccgttgtaac taaagccaat ttcatctttc
    65341 acaaacttac tgaaatcttt cagagtcatg gaagaataaa tcgagcctgt ctcactggca
    65401 tttttctctt catttttgac cagtacagca tcaggttttg cggcactact ggtcgggaac
    65461 ggattggcgg catacccttg atcattcgta cttacgctac gattcaccgt cgctggcgta
    65521 tattttttct tctcttcatc tttatacttt ttgagttcgc tttgcgtttc tcttaacgct
    65581 ttttctaata actcaaggcg atgttccacc gttaatgatt cagccaatga ttgtgcagaa
    65641 aaggctaacg gtgccattaa taagatggca gaggtaataa gatttcgtct aaacataata
    65701 tccctttatg gtgcaaagaa agaattaacg tatcgcatcc agactgttct gaatgcgacg
    65761 ataattaagg tgctttattg gttatttttt taatgacagc ccccgcgttt tgatcacttc
    65821 tgcataccat ccgaagcttt tcttacgcgt tcttgtgagg cttccttcgc cattatcatc
    65881 acgatccaca taaataaagc cgtagcgctt agacatttgt gaatgagatg cactgactaa
    65941 atcaattggc ccccaactgg tgtaccccat aatatccaca ccatcggcaa tcgcttcatt
    66001 tacctgtacc aggtgatcgt ttaaataggc aattcgataa tcgtcctgta tcgaaccatc
    66061 cgcttcaacg ctgtcttttg cgcctaatcc gttctcgaca ataaataacg gtttttgata
    66121 acgatcccaa agcgtattta acagaacccg taatccaacc gaatcaattt gccaccccca
    66181 ctctgaactt ttcagatgcg gattggggat catattcagt atgttgccct gcgcattttt
    66241 attaatgctt tcgtcgtggg aaacacaacc agtcatgtaa taactaaaag agatgaaatc
    66301 gacggtatgt tttaaatctt ctgcgtcact ttcagtcatc tcaatggtga tattgtggtc
    66361 gcggaagaaa cgctgcatat agccgggata ctggccacgc gcctgaacat caccaaagaa
    66421 catccagcgc cggttctctt ccatggcctg caacatatcc tgtggctggc aggtgagggg
    66481 gtaaaccagc ccaccgagaa gcatattgcc gattttcgct tcggggagca ggctatgaca
    66541 ggctttaact gcccgcgcac tggcaaccag ttgatggtgg atagcctgat aaacttccgc
    66601 ctcgccactc tcttctgcca gccccacgcc cgtgaatggc tcgtgtaacg acatgttgat
    66661 ttcattaaac gtcagccata acgccacttt atgttggtag cgagtaaaga ccgtgcgggc
    66721 gtaatgctcg aagtgatcga tgaccgctcg attagcccaa ccgccgtagt ttttcaccag
    66781 cccatatggc atttcgtaat gggataacgt taccagcggc ttgatccccg cctgcgccat
    66841 ttcatcaaac agccgatcgt aaaacgctaa ccccgcttca ttcggttcga cttcgtcgcc
    66901 ctgagggaaa attcgcgccc aggcaatgga aatacgcaga caggtgaagc ccatctcggc
    66961 aaataacgcg atatcttccg ggtaacggtg ataaaaatcg atggcgacat ctttgatatt
    67021 ctctttcccc aggatgcgcg gttccatttt tcccattacg ccatgaggct gtaaatctga
    67081 ggtcgagatc cctttgccat cttcctgcca ggcaccttcc acctgattgg cagctgttgc
    67141 gccaccccaa agaaatgttt ctggaaatgc tttcataatt aactcctttt atcgttagcg
    67201 aatgatggat aacagcggtt cacctgcgct tatctgcgcc gtgccgtggg gtaatacgtc
    67261 cgtaaaatca tcgctattac tgattaatac cggcgtcgtc agatcaaatc cggcctcgcg
    67321 aatagcaggg atatcaaaag aaatcagccg atcgcctgta ttgaccttgt cacccacgtt
    67381 gacgtgagcg gaaaagaatt tgccgtccag ttttacggtg tcgataccga catgaatcag
    67441 gagctccaca ccatcatctg actcaatgcc aatggcgtgt aatgtggcga acaacgaagc
    67501 aattcgaccc gcaaccggag aacgcacttc accaaccgag ggcagaatgg cgataccttt
    67561 acccaacagg ccactggcaa acgtggtatc agcgacgtga atgagcggca caatctctcc
    67621 cgtcatcggt gaacagatac cgccctgctc aggtggtgta ataacctctg gtgttttctc
    67681 ttgcggggca ccctgcgctg gctgacgttt agcggtgatg aaatgaagca tcaccgtacc
    67741 gacaaatgcg caaccgatgg caatgacacc gccaataacg ctggcccaga cggtgaaatc
    67801 aattcccgtt gacgggatgg tttgcatgaa ggtgaaaata cttggcaaac caaaggagta
    67861 cactttcgtt tgcgcgtagc caatgatggt ggcccccaaa gccccactga tacaggcgat
    67921 aacaaagggg tacttacgcg gcagattaac gccatatacc gctggttcgg tgataccaaa
    67981 caggcccgtc aacgccgctg atcccgccac cacttttttc tgcgcatcgc gttcgcagag
    68041 gaagacgccg agcgccgccc cgacctgcgc cataatggcg ggcattaaca gcgggatcat
    68101 ggtgtcgtag cccagcacgg tgaagttatt gatacacagc ggcaccaggc cccagtgcag
    68161 tccgaacatg acgaagattt gccagaagcc gcccattacc gcgcctgcaa atgcaggaac
    68221 cgcctgataa agccagagat aaccggcggc aatcagttcg cttatccagg ttgatagcgg
    68281 ccccaccagc agaaaggtga cgggtgtgat aaccatcaga catagcaatg gtgtgaagaa
    68341 atttttgatt gccgacggta accacgcatt aagtcggcgt tccagaatgc tgcacaacca
    68401 ggcagaaaaa ataatgggaa taaccgatga cgagtaattc aacaatgtga ccggaatacc
    68461 caggaaatcc agccccagct catccgcttt ttgcccgttc tcgaaagcag tcagaattaa
    68521 tggatgcact aacgctccac caatcaccat ggcagtaaat ggattaccgc cgaagcgttt
    68581 ccccgcggtg tatcccagga ttatcgggaa gaaccaaaac aaggcatcac tggcgctgaa
    68641 taaaattaaa taagtaccac tttgttcggt cgtccactga aaagtgagcg ccagagccag
    68701 catacctttc aggatcccgg ttgccgccat caaaccgatc agaggcgtaa aaatacctga
    68761 aataacataa acaaagcggt ttagcagatt acctttctca tcattttccg gtgcctgttg
    68821 cgctttttcg ccaaggcctg ccacgctgtt gaccgccagg aagacatcgg ccacatggtt
    68881 acctatgacc acctgaaact gaccaccgct ttccaccacc ataataatac cgggggtctt
    68941 tttcagtacc tcagcttgcg ctttgctttc atcctttaac ttaaaacgta atcgcgttgc
    69001 gcaatgcatc agactcacaa tgttatctgc gcccccgact cctgcgacta tttttctggc
    69061 taactccgtc ataacttgcc ctctaccgct ttgcggcaaa actccaaaaa aaaacctgaa
    69121 aaaaacggcc tgacgtgaat caagcaattt ttttcaggtt ttgcccgctt agtgcggtaa
    69181 caatccttta ctcagtaata atatttcagt gttctttgcg cacgcgctct atatttatgg
    69241 ctaaaaacat aatctctgcg ggtgaaattt tacgttgata ctgcaaacca ataaaaatgg
    69301 cgatccgttc cgcacattgc catgcttgcg ggtaattttg ttttactgct tgttgtaatg
    69361 attcatcact atcgttaatt gaagcatgtt caagaatacg ccaggataaa aacttcagat
    69421 gtgtaaccag tcgctgataa ctcaagcttt cttcctggta attaaggctg aactgaaatt
    69481 ttattaattg cagcatttcg cgcattaact gcgtgacacc tgcaacatcc tccatatttc
    69541 cgctcatttg ggcactgacc agatgcatgg caataaagcc cacttcatct ttcggtaact
    69601 gcacgcccaa ccgtttatca ataatggtta acgcttcttc ccctagctgg aactctttcg
    69661 ggtaaagccg ctggatatcc cacagcaacg ggttgggcag caacacgttt tgctgaaagc
    69721 gtttaatcgc aaactggcaa tggtcagtta gcgagatata aatactgtcc tgtaattttc
    69781 ccaagcgctc ctgcgctaaa gagataatac gatcacaggt tgccatcacc tcaagaggaa
    69841 tatgacttaa gagttcgctt aatcgcccgt tcagttcatg actgctcaag gcatactctt
    69901 tttctattcc acttgagtta attctttcgc cagcgcgttt ttgaaagcca attcctcgcc
    69961 ccatgacgac tttttcccgc tgttgatcat caataaccac cacaacatta ttgttgagaa
    70021 ttttggtgat ttgcatgttc atagcaagga ccttttttat aaacaaaaaa acccgacttc
    70081 accagtattc tctggttatg tcaggttttg cctgcgaatg cagtaacaat ccagtcattt
    70141 attaatggtt tttataacga acatccaggt tcggaaatta atttaattgc gtgcagagaa
    70201 aatagcaatg cgctattgat aaaaatatga ccatgctcgc agttattaac tttgtgtaat
    70261 tttaggaatt tataaagtta tatataacaa atcccaataa ttaagttatt gggatttgtc
    70321 tggtgaatta tttgtcgcta tcttttcccg ccagcagttt atccagctca tcaccgccta
    70381 catgacggaa atcctgcccc ttcacgtagt agaagataaa ctcacaaata ttctggcagc
    70441 ggtcgccaat acgttcgata gaacgcgcgc agaacagcgc agtaagtacg ctcggaatgg
    70501 tacgcgaatc ttccatcatg taggtcatca gttgacgaac aataccttcg tattcctgat
    70561 cgactttttt atcttcacga taaatacgta ccgcttcgtc aatgtccatc cgcgcgaacg
    70621 cgtccagcac gtcgtgcagc atctggatgg tatgacggcc cagcgactcc agacttacca
    70681 gcaacggctg atgctgctgg gagaatttct ccagcgcagt acggcagatt ttgtccgcca
    70741 cgtcgccaat acgctccagc tcggcaatgg ttttactgat caccataacc agtcgcaggt
    70801 cgctcgccgt cggctgacgt ttggcgataa tgcgcacgca ggcttcatcg atcgccactt
    70861 ccatcatgtt gacgttcttg tcgccttcga tcacgcgctt cgccagatcg ctgtcctggt
    70921 tatgcatcgc ggtgattgca tcagaaagct gctgctccac catgccgccc atggtcatca
    70981 cctgcgtgcg gatactttcc agttcggcgt tgaactggcc ggaaatatgt ttattaagat
    71041 tgagactgtc cataacgcac tcctgaatca accgtaacga ccggtgatgt agtcttctgt
    71101 ttgtttcttc gctggcttgg tgaacagatc gtccgtgttg ctgaactcaa tcaattcgcc
    71161 caggtacata aacgccgtgt ggtcggaaca acgcgcagcc tgctgcatgt tgtgggtgac
    71221 gatcaccacg gtgtaatcct gcttcagttc ggtgatcagc tcttcaatac gcccggtaga
    71281 gatagggtcg agcgccgaac acggttcgtc gagcagcagc acttccgggc gaatggcgat
    71341 accacgcgca atacacagac gctgttgctg accaccagag agagagtaac cgctctggtg
    71401 caatttatct ttggtttcgt tccacaatgc ggctttggtc aatgcccact gcacgcgctc
    71461 gtccatgtcg gcacgggaga gcttctcaaa cagacgaacg ccaaaagcga tgttgtcgta
    71521 gatggacatc ggaaacggcg tcggtttctg gaacaccatg cccactttcg cacgcagcag
    71581 ggcgatatcc tgagagttgg tcaggatgtt gtcgccatca agcagaattt caccttccgc
    71641 acgctgctcc gggtacagtt caaacatttt gttgaaggta cgcagcagcg tcgatttacc
    71701 gcagccggac ggcccgataa acgccgttac ctggttttta gcgatatcca ggttgatgtt
    71761 tttcagggca tggaatttgc cgtagtagaa gttcaaatta cgaacctgaa ttttactcgg
    71821 ggcagtttca accatactca ttgcaatctt ttcctcatcg tgcctgatgc acttcgctaa
    71881 tcaggcttac cgtaggccgg atcaggcgtt cacgccgcat ccggcaaagt gttaaccgtg
    71941 tttattcttc gcaaaaacaa cgcgcgccag aatgttcagc agcagtacgc acagggtaat
    72001 gatcaatacc ccggcccagg ccagttgctg ccattccgca aacggactca tcgcaaactt
    72061 aaagatcgtc accggcaggt tggcgatcgg ctgcatcatg tccgtgctcc agaactggtt
    72121 ggagagcgcg gtaaacagca gcggcgcggt ttcaccagca atacgggcaa tcgccagcaa
    72181 gataccggtc atgatcccgg acaccgacgc tttcagcgta atcgcggaga tcatcttcca
    72241 cttcggtgta cccagcgcat aagccgcttc acgcaggctg tccggcacca gtttcagcat
    72301 gttctcggtg gtgcggataa caatcggcac ctgcaacagc gccagcgcaa ttacgcccgc
    72361 ccagccggag aagtgctcca tctgtgccac cacgatggtg tagacaaaca gaccaaccac
    72421 aatcgacggc gcggagagca gaatgtcgtt aatgaagcga atcacttctg ccagccagga
    72481 tttacgacca tattccgcca gataaatccc cgccataatg cccagcggcg taccgaatac
    72541 cgtggcccac aaaattaaca gcccgctacc cgccagggcg ttcgccagac caccaccttc
    72601 cgtattgggc ggcggcgtca tttcagtgaa cagcgccagc gacataccgt cgataccgcg
    72661 agtgatggtg gacattaaaa tccagatcag ccagaacagg ccgaaggcca tcgtcgccat
    72721 cgagagcgtc agcgcaatac ggtttttgag gcggcgacgc gcctgcattt tgcggcgaga
    72781 ttcagccagc gccgcagtgg tttgcatttc aaccatagcc attagcgtgc cccctcattc
    72841 ttagccaggc gcataatcat aaacttcgat gcggcgagga cgatgaaggt aatcacaaac
    72901 aggatcaggc ccagttccat cagtgcggca acgtgcagac cggattccgc ttccgcaaat
    72961 tcgttcgcca gcgcagaggt gatactgttg cccggcatat acagcgaggc gctgtcgagc
    73021 tggtaggtgt taccgatgat aaaggtcacc gccatggttt caccgagcgc gcggcccagc
    73081 cccagcatga tgccgccgat aacaccattt ttggtgaacg gaagaacgat acgccagata
    73141 acttcccagg tggtgcagcc aataccgtag gccgactctt tcatcatcac cggggtttgt
    73201 tcgaacacat cacgcattac cgccgcaatg tacggaataa tcatgatggc gaggatcacg
    73261 cctgccgcga ggataccgat accaaatgcg gggccagaga acagcgcgcc aacaatcggg
    73321 atattcgaca tgatattgcc gaccggctcc tgaaagtaaa cggcgaacag cggcgcaaag
    73381 ataaacaggc cccacatgcc gtaaacgata cttggaatgg ctgccagcag ctcaatggcg
    73441 atacccagcg ggcgtttcag ccagccaggc gcaagctcag tcaggaacag ggcgataccg
    73501 aaactcaccg ggacggcgat cagcagcgcg ataaacgaag tcaccaacgt accgtagatc
    73561 ggcaccagcg ccccgtagat atcgttcggt gcatcccact ctttggtcca taggaaagcc
    73621 agaccaaatt tctgaatgct cggccaggag gagatgatca gagagacaat aatgccaccc
    73681 aacatcaata gcacaatcag cgccgccagt tttaccagca cgctgaaaat tatgtcgccc
    73741 tttttacccg gtgggttaaa agcaggcttg gttgcagcca taagttactc ttcagttaaa
    73801 cgcgtttaca aagtcatttt gtaatgccgg atgcggcgta aaacaccgta cccggcctgg
    73861 agttttatta gtacagcggc ttaccgctac tgtctttaat attggtcttc cacgcagcgc
    73921 gaacctgttc aactacacta tccggcaggc tggcgtaatc caggtcgttc gcctgtttag
    73981 ccccggtttt gtacgcccag tcgaagaatt tcagcacttc tgtgccttgt tctggtttct
    74041 tctgatcttt gtggatcaga atgaacgtgg tagaggtaat aggccatgca tcttcgcctt
    74101 tctggttggt cagatcctga gcgaaggttt tgctccagtc tgcacctttt gctgcattag
    74161 cgaagttttc ttcggtcgga ctaaccggtt taccatcagc ggagatcagt ttggtgtacg
    74221 ccaggttgtt ctgcttcgcg taagcatatt caacataacc aattgcaccc ggcagacgct
    74281 gaacgaacgc ggcgataccg tcgttacctt taccgcccag accgatcggc cattttacgg
    74341 tagagccagt accaacgttg tttttccact cttcgttcac tttcgccagg tagctggtga
    74401 agacgaagga agtcccggag ccatctgcgc ggcgtactac agcaatgttt tgtgaaggca
    74461 gtttcagacc cggattcagt ttggcgatgg cttcatcatc ccacttcttg attttgccca
    74521 ggtagatgtc gccgagggtt ttaccatcca gcaccagttc gccagacttc agccctggaa
    74581 tgttaaccgc cagcaccacg ccgccaatca cggtcgggaa ctggaacaga ccttcctgag
    74641 ccagtttttc gtcagacagc ggcgcgtcag aggcaccaaa atcaacggta ttagcgataa
    74701 tctgttttac gccaccggaa gaaccgatac cctggtagtt aactttatta ccggtttctt
    74761 tctggtaagt gtcagcccat ttggcataca ccggcgcagg gaaggttgca cctgcacctg
    74821 tcaggcttgc ttctgcaaac acagagaaag cgctcatcga taaggtcgcg gcgacaacag
    74881 ttgcgacggt ggtacgcata actttcataa tgtctcctgg gaggattcat aaagcattgt
    74941 ttgttggcta cgagaagcaa aataggacaa acaggtgaca gttatatgta aggaatatga
    75001 catttttatg acagaaagga attaccaaat aacttactga ttttaaatgt gaagatgaaa
    75061 atcccacatg tcatcttttt gtcatgatgc atttaaaagt gataaagaaa tgggtgagat
    75121 gacaacgatg tgatgaacaa tcgttgtcag acctattagc gcacaatctc aacagttatc
    75181 gtcgctgttg cagtaaattg ccccggggat gggcgagctc cagtaaggct cgcaggagca
    75241 gctgaaaaag ttacactacc tgatttttca ccatacactg ttgatttacc catgtctgtt
    75301 ggcaactcac caccgttcac tttgatttcc ttattatttt tgtcgtaaat ttttatcccc
    75361 acgttagagt ttgaagttac gaccattgaa ttgtccgtat tgggttcggt agcataaaaa
    75421 gacgtactga tatcatcggt attcagtatc cccaggtttt cacactcaat agtgaccgtt
    75481 ttagtaattt tatgtgttgt taccgcactt ccaacaacag atgaaaaatc tgcagctggt
    75541 atttctttaa aatcaaaata aactggctgt aaattattta tttcgcaact ttgcggagct
    75601 gtaatatcgc cccgaatata aacatcagcc atcggctgtg agtaatccac cggggtgtca
    75661 cggctaattg ttccatacaa tgtcactatc tttgtttcag gaataatcaa ttttcctgca
    75721 aatgtcttct tgatataaaa atatactatc gctttagccc cactggatat acttaaaggt
    75781 tcaatgcggt tgcaattgta ctttgtatta ccagacgggt tgttagcatg atattcaaaa
    75841 ggtgcattca catacccaac cccaagaata tctatagcaa tcccaacatc aagattcgga
    75901 ttaagatcat aataattaat attagagtga accccagcat aagttaatgc ctggtttatt
    75961 tttgacgtat aatagatata ggggaattct cctacgttac cagtatcaca atgacagcgt
    76021 ccaggatatg aggatccatt agttacagtg gtgttaaatg tcgttccaac cttattttcg
    76081 ttcgcggtaa taagatatcc cgagaaatca agcgtactgt gaaatacgcc attatcaggg
    76141 gtacagatac ctttaggagc tgcaagtgct acactactta acgagagcat aaacacagca
    76201 aagcaccact gtttgatata cttgttcata aaaatccttt taatgacatg tcacaccctg
    76261 ttcaattaat ccgctctcac tttctggtgt cgaaagtgaa taattgaaga tgcattttgt
    76321 tgaggcgttt tctccccagg ataacgtaac ctgtcctttt tctggtagac cagaaagata
    76381 aagttcgcca cggtcaccga caattcctga atctgccaaa cttgcatctt gtatcgcagc
    76441 aatggctcca aaaggtaccg gtttaccatt tgcatttaac actctgaaga gaacgcgata
    76501 acccacatga gtcacaaact ccgctttaac cactgcacca cgcgtggaaa ccatcgtaac
    76561 gacggtatta tcaagttcta tattcgactt cgagaaatag ttatcgctga ttgctacccg
    76621 attttcatca taagggttta gctgtgtctt gaccgtatag ccgcgccagt ccgtcgcagc
    76681 accgcgcata ttatctattt ctaacccggc ggccccaggt gctttaacaa gagcaatagt
    76741 ttcacctaac tcctgactta acgttacccc ttcgctgtgt gccagaattc cccctctgat
    76801 accataattc agcctctggg aattttggct gtagttatag ctgccgttca ccgtgccata
    76861 agagccatca taggtcaggg acatattacc gccaacctca tcatcatgac tgttatagga
    76921 ctgcattaaa ttccatgaaa ggttatttcg ttccagcgcc gtaccgctga gtccgagttg
    76981 atgtgagcta ccggaattat cgttttgcga aacagaatag gtggcgtaac tattacccca
    77041 ccagcccgat aaaggaacag ataaagagag catgtagatc tgctcactgg tgttatcact
    77101 ggcatggttg ttccccgtcc cctgtctggc agtattggaa taactccagg agacgttcca
    77161 cgagagacgc ttccagctac tgctgtagcc aaattgcatt aatcgttcga catcatcggt
    77221 atgccagtat tgttgccggc ttaatgtcag gtataaattg ccataatctc tccccaacga
    77281 ctgcgttagc gttccctcca cgcgactacg tcggttacct gtttcccaaa agtcttcagg
    77341 gctgttgcgc cgcgatgccc actcattgag ggtatagtag ccctcggtgg agtaacgata
    77401 gccaaccagg cgcaagctag tgtctgtgtc gtcaaatcgt tttgaataga gaaagcgata
    77461 tgactgaccc gtttctgtat catcatggct aaaattcgca cgagcatgtg tcacatcgaa
    77521 agaaattgcg cccaaagcgc caagatcttt accgacaccc agagctgcag aaccataatt
    77581 ttcagcagcc tgcatacctc catataaagt gatcccgtgg gaaaaaccat gaagtatttg
    77641 cgcctgaagg acgtccggtg taaatccgtc ttcatcgcgc aattcaccca cgctgacatc
    77701 aacatctgtc tgaccttcac gtttaagaat cgccaatgaa gcataaggtt gggtgaatcg
    77761 ttgttcgcta ccgtcttctt ctttgatggt tacgtccaaa tcgccacctg tactggcggt
    77821 gttgagatcg gtgatctcaa aagcaccttg tggaacggca gactgataaa tcacgtagcc
    77881 attctgccga atgatgacgg tggcgttgga ctttgcaatc ccacgaacca caggagcaaa
    77941 gccattctgg ctggcgggta gcatatcgtt atcagtatac aaacgcgcgc cgcgaatttg
    78001 cgtactgtcg aaaatatcgc tcgccgtcca cgtatcacca atcataatct ggcttcttaa
    78061 cgcggcaata tcgcgctgta tccaggtact aatggagttg tattccgcac ggccatcgct
    78121 ggtgtttaaa gtactataat tacgtagccg ccacggtcca atattcagtc cattgcgcag
    78181 attgagataa ctggtatctg tctcttgtga accataatct gaacgtagtg tatgtgaacc
    78241 agaaaaatcg tagttgatgg tgagcgcgtt aatcccctca tcccaacgcg tggggctgat
    78301 gtaatcataa gccatcgcgt ccatgtagat ttggggaatg gatatattca gctgcaattt
    78361 atcactgtca aactgccagg atgagccttc aatacgtgga gcaggatcga ggcaaaccgt
    78421 ctcatccaca tcattgaacg atttgagcgc atcaactttt atgccaaagg aggacagtgt
    78481 ttttttaatg gcaataaagc aagcgtgcag atcagcatct ggattatcct gaacaaatgt
    78541 cacatcctga cgttcaaaca ttttaccgtt gataaaaata tcaacgctat aaacacctgg
    78601 gggcatcagg tttgcccgat taaactggcg aatatcgact ccggattgat ggtcgatttc
    78661 cagaagcgcc ggattgaata cactgacctc ggccatcagg ttttgcgaga aaatcagaca
    78721 cgtccctgcc gtgaggccaa ccactattct ggtcgtcatc ataatacgcc tatccttatc
    78781 ccttgcttac agcgtcattt ttatcgggtc actgactgcg ccgtaatcat taataaatcg
    78841 ccaggtcatt tggcctcccg cagaattagc aggtaaaggt aacgtgagcg tttcaaaagg
    78901 acggatcata ttgaggggca catcatggcc atcaacgatg acatccatca cactgatatg
    78961 aattgggttg ggattctctg ctatcagatt tcgcccttcc cggcgccatt tcactttcgg
    79021 cgcagcatct tcgggaataa ggcggatatt tgctggtcgc cagaacagct tgatacgcgt
    79081 ttttactgct atttccagcg agttcgtcgc tttgttgctc gctgatggcg gaatattttt
    79141 aatattcagc cagaagactg actcgcgatc atcaggcaat gacacacctt tggtgtgaat
    79201 aatgcgtaat attttttctt gcgaaggttc taatcgtgat actggcggaa taacggtaaa
    79261 cggcgctttg cttttatcct ctttattaaa tggatctacc caagcctgga tgagataagg
    79321 tattttttct tctttgctaa atacagaaat agatttatca gcattattac ttaagtaaac
    79381 cacccgcgta ccaccaatga caacgcctgc ataagttaat gctggttggc ctaaaaaaac
    79441 gctgagtata atcgcggtaa ataacttaca tgaaactgtt gttttaaaga gcctgattaa
    79501 acaacttttt ctggacataa cgactccctg gccaaagcca actgaataaa aaggcccttg
    79561 ataaattatt tcttatattc gacagtaaat tgtgaatcag cgttcgccgt accagccgtt
    79621 acggtcgcat tggttgccac atagcgtgca ataaacttca tttcagcctt gccttcagca
    79681 tcgatagcgg tataagcaga gtcgttataa agtttaacct gcgaattatc ggactggtta
    79741 aaaatacgaa taccaacgcc ggttgccgca atcgcttgtc ctgtaccggt aaaatcacca
    79801 gggttgcctg ctgtaagcgg cgtacccact ttcaggtcgc cgtcgccgcc aggcgcttct
    79861 gtaccatcaa aacgaacggc tgcttgagtg tacgttgccg ggcaattttc aagcttaata
    79921 ctgaacttct gtggcgacgc cagactacct acacctgaaa atgcattttt actgacttta
    79981 cccagtggga caatttgatc ggctgtatcg gaggtaactt cacaagtagt gtcgacaatt
    80041 tcaccgacga agtgaacaac accatcttct gccagtgcat gcccggccag cattagagat
    80101 gccagagtga atgcaccgcc tataatatta cgcttcataa aaactccatt gatataatat
    80161 atcctttacc atgaatcatc acttatatta ataagtgtat ttttgatt
//
LOCUS       DAZGJW010000004        78896 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZGJW010000004 DAZGJW010000000
VERSION     DAZGJW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 78896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.004"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..812
                     /gene="ynaI"
                     /locus_tag="ACHKIK_000278"
     CDS             <1..812
                     /gene="ynaI"
                     /locus_tag="ACHKIK_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO9081517.1"
                     /translation="PGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGRDITSARIM
                     SRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFD
                     RPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGRMTN
                     RRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYC
                     FTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
     gene            complement(863..2476)
                     /gene="mppA"
                     /locus_tag="ACHKIK_000279"
     CDS             complement(863..2476)
                     /gene="mppA"
                     /locus_tag="ACHKIK_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO9081518.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASMENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2813..3712)
                     /gene="pgrR"
                     /locus_tag="ACHKIK_000280"
     CDS             complement(2813..3712)
                     /gene="pgrR"
                     /locus_tag="ACHKIK_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO9081519.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGHHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            3850..4770
                     /locus_tag="ACHKIK_000281"
     CDS             3850..4770
                     /locus_tag="ACHKIK_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9081520.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVSYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSIFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            4770..5702
                     /locus_tag="ACHKIK_000282"
     CDS             4770..5702
                     /locus_tag="ACHKIK_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFO9081521.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQKALEKVIPFFNTHLG"
     gene            5720..6358
                     /locus_tag="ACHKIK_000283"
     CDS             5720..6358
                     /locus_tag="ACHKIK_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9081522.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVSALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            6667..7395
                     /gene="mpaA"
                     /locus_tag="ACHKIK_000284"
     CDS             6667..7395
                     /gene="mpaA"
                     /locus_tag="ACHKIK_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO9081523.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPATAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(7370..8335)
                     /gene="ycjG"
                     /locus_tag="ACHKIK_000285"
     CDS             complement(7370..8335)
                     /gene="ycjG"
                     /locus_tag="ACHKIK_000285"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO9081524.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVTTAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFVDLDGPTWLAVDVEPALQFTTGELHL"
     gene            8454..8960
                     /gene="tpx"
                     /locus_tag="ACHKIK_000286"
     CDS             8454..8960
                     /gene="tpx"
                     /locus_tag="ACHKIK_000286"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO9081525.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(9004..10545)
                     /gene="tyrR"
                     /locus_tag="ACHKIK_000287"
     CDS             complement(9004..10545)
                     /gene="tyrR"
                     /locus_tag="ACHKIK_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO9081526.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(10693..11754)
                     /gene="ycjF"
                     /locus_tag="ACHKIK_000288"
     CDS             complement(10693..11754)
                     /gene="ycjF"
                     /locus_tag="ACHKIK_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO9081527.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(11751..13148)
                     /gene="ycjX"
                     /locus_tag="ACHKIK_000289"
     CDS             complement(11751..13148)
                     /gene="ycjX"
                     /locus_tag="ACHKIK_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO9081528.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            13303..14301
                     /gene="ycjW"
                     /locus_tag="ACHKIK_000290"
     CDS             13303..14301
                     /gene="ycjW"
                     /locus_tag="ACHKIK_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9081529.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKAEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(14412..15317)
                     /gene="ompG"
                     /locus_tag="ACHKIK_000291"
     CDS             complement(14412..15317)
                     /gene="ompG"
                     /locus_tag="ACHKIK_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO9081530.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(15362..16444)
                     /locus_tag="ACHKIK_000292"
     CDS             complement(15362..16444)
                     /locus_tag="ACHKIK_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9081531.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(16458..17117)
                     /gene="pgmB"
                     /locus_tag="ACHKIK_000293"
     CDS             complement(16458..17117)
                     /gene="pgmB"
                     /locus_tag="ACHKIK_000293"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO9081532.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(17114..19381)
                     /gene="ycjT"
                     /locus_tag="ACHKIK_000294"
     CDS             complement(17114..19381)
                     /gene="ycjT"
                     /locus_tag="ACHKIK_000294"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO9081533.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(19378..20433)
                     /gene="ycjS"
                     /locus_tag="ACHKIK_000295"
     CDS             complement(19378..20433)
                     /gene="ycjS"
                     /locus_tag="ACHKIK_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO9081534.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(20443..21231)
                     /gene="ycjR"
                     /locus_tag="ACHKIK_000296"
     CDS             complement(20443..21231)
                     /gene="ycjR"
                     /locus_tag="ACHKIK_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO9081535.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLHVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEEPAQAYRDSLAWLRTC"
     gene            complement(21249..22301)
                     /gene="ycjQ"
                     /locus_tag="ACHKIK_000297"
     CDS             complement(21249..22301)
                     /gene="ycjQ"
                     /locus_tag="ACHKIK_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO9081536.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNLIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFAKSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(22332..23174)
                     /gene="ycjP"
                     /locus_tag="ACHKIK_000298"
     CDS             complement(22332..23174)
                     /gene="ycjP"
                     /locus_tag="ACHKIK_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO9081537.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(23161..24042)
                     /gene="ycjO"
                     /locus_tag="ACHKIK_000299"
     CDS             complement(23161..24042)
                     /gene="ycjO"
                     /locus_tag="ACHKIK_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO9081538.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(24063..25355)
                     /gene="ycjN"
                     /locus_tag="ACHKIK_000300"
     CDS             complement(24063..25355)
                     /gene="ycjN"
                     /locus_tag="ACHKIK_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO9081539.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(25369..27048)
                     /gene="ycjM"
                     /locus_tag="ACHKIK_000301"
                     /pseudo
     CDS             complement(25369..27048)
                     /gene="ycjM"
                     /locus_tag="ACHKIK_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(27260..27574)
                     /gene="pspE"
                     /locus_tag="ACHKIK_000302"
     CDS             complement(27260..27574)
                     /gene="pspE"
                     /locus_tag="ACHKIK_000302"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO9081540.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(27649..27870)
                     /gene="pspD"
                     /locus_tag="ACHKIK_000303"
     CDS             complement(27649..27870)
                     /gene="pspD"
                     /locus_tag="ACHKIK_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO9081541.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(27879..28238)
                     /gene="pspC"
                     /locus_tag="ACHKIK_000304"
     CDS             complement(27879..28238)
                     /gene="pspC"
                     /locus_tag="ACHKIK_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO9081542.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(28238..28462)
                     /gene="pspB"
                     /locus_tag="ACHKIK_000305"
     CDS             complement(28238..28462)
                     /gene="pspB"
                     /locus_tag="ACHKIK_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO9081543.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(28516..29184)
                     /gene="pspA"
                     /locus_tag="ACHKIK_000306"
     CDS             complement(28516..29184)
                     /gene="pspA"
                     /locus_tag="ACHKIK_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO9081544.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            29351..30328
                     /gene="pspF"
                     /locus_tag="ACHKIK_000307"
     CDS             29351..30328
                     /gene="pspF"
                     /locus_tag="ACHKIK_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO9081545.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(30448..31713)
                     /gene="puuE"
                     /locus_tag="ACHKIK_000308"
     CDS             complement(30448..31713)
                     /gene="puuE"
                     /locus_tag="ACHKIK_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO9081546.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(31751..33031)
                     /gene="puuB"
                     /locus_tag="ACHKIK_000309"
     CDS             complement(31751..33031)
                     /gene="puuB"
                     /locus_tag="ACHKIK_000309"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO9081547.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(33033..34520)
                     /gene="puuC"
                     /locus_tag="ACHKIK_000310"
     CDS             complement(33033..34520)
                     /gene="puuC"
                     /locus_tag="ACHKIK_000310"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO9081548.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLTDLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGESNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(34795..35352)
                     /gene="puuR"
                     /locus_tag="ACHKIK_000311"
     CDS             complement(34795..35352)
                     /gene="puuR"
                     /locus_tag="ACHKIK_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO9081549.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(35379..36143)
                     /gene="puuD"
                     /locus_tag="ACHKIK_000312"
     CDS             complement(35379..36143)
                     /gene="puuD"
                     /locus_tag="ACHKIK_000312"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO9081550.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            36355..37773
                     /gene="puuA"
                     /locus_tag="ACHKIK_000313"
     CDS             36355..37773
                     /gene="puuA"
                     /locus_tag="ACHKIK_000313"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO9081551.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            37922..38086
                     /locus_tag="ACHKIK_000314"
     CDS             37922..38086
                     /locus_tag="ACHKIK_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081552.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            38076..39461
                     /gene="puuP"
                     /locus_tag="ACHKIK_000315"
     CDS             38076..39461
                     /gene="puuP"
                     /locus_tag="ACHKIK_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO9081553.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALFVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            39595..39840
                     /gene="ymjA"
                     /locus_tag="ACHKIK_000316"
     CDS             39595..39840
                     /gene="ymjA"
                     /locus_tag="ACHKIK_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFO9081554.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            40153..41796
                     /gene="sapA"
                     /locus_tag="ACHKIK_000317"
     CDS             40153..41796
                     /gene="sapA"
                     /locus_tag="ACHKIK_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO9081555.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            41793..42758
                     /gene="sapB"
                     /locus_tag="ACHKIK_000318"
     CDS             41793..42758
                     /gene="sapB"
                     /locus_tag="ACHKIK_000318"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO9081556.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            42745..43635
                     /gene="sapC"
                     /locus_tag="ACHKIK_000319"
     CDS             42745..43635
                     /gene="sapC"
                     /locus_tag="ACHKIK_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO9081557.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            43635..44627
                     /gene="sapD"
                     /locus_tag="ACHKIK_000320"
     CDS             43635..44627
                     /gene="sapD"
                     /locus_tag="ACHKIK_000320"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO9081558.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            44629..45435
                     /gene="sapF"
                     /locus_tag="ACHKIK_000321"
     CDS             44629..45435
                     /gene="sapF"
                     /locus_tag="ACHKIK_000321"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO9081559.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            45503..45856
                     /gene="ycjD"
                     /locus_tag="ACHKIK_000322"
     CDS             45503..45856
                     /gene="ycjD"
                     /locus_tag="ACHKIK_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFO9081560.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            46224..47012
                     /gene="fabI"
                     /locus_tag="ACHKIK_000323"
     CDS             46224..47012
                     /gene="fabI"
                     /locus_tag="ACHKIK_000323"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO9081561.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            47157..48284
                     /gene="yciW"
                     /locus_tag="ACHKIK_000324"
     CDS             47157..48284
                     /gene="yciW"
                     /locus_tag="ACHKIK_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO9081562.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            48352..50286
                     /gene="rnb"
                     /locus_tag="ACHKIK_000325"
     CDS             48352..50286
                     /gene="rnb"
                     /locus_tag="ACHKIK_000325"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO9081563.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATAMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(50364..50468)
                     /locus_tag="ACHKIK_000326"
     CDS             complement(50364..50468)
                     /locus_tag="ACHKIK_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081564.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            50522..52507
                     /gene="pdeR"
                     /locus_tag="ACHKIK_000327"
     CDS             50522..52507
                     /gene="pdeR"
                     /locus_tag="ACHKIK_000327"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO9081565.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWREKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(52489..52617)
                     /gene="ymiD"
                     /locus_tag="ACHKIK_000328"
     CDS             complement(52489..52617)
                     /gene="ymiD"
                     /locus_tag="ACHKIK_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO9081566.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            52655..52828
                     /gene="yciZ"
                     /locus_tag="ACHKIK_000329"
     CDS             52655..52828
                     /gene="yciZ"
                     /locus_tag="ACHKIK_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO9081567.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            52918..53667
                     /gene="yciT"
                     /locus_tag="ACHKIK_000330"
     CDS             52918..53667
                     /gene="yciT"
                     /locus_tag="ACHKIK_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO9081568.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            53936..54154
                     /gene="osmB"
                     /locus_tag="ACHKIK_000331"
     CDS             53936..54154
                     /gene="osmB"
                     /locus_tag="ACHKIK_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO9081569.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(54280..54606)
                     /gene="yciH"
                     /locus_tag="ACHKIK_000332"
     CDS             complement(54280..54606)
                     /gene="yciH"
                     /locus_tag="ACHKIK_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO9081570.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(54606..55343)
                     /gene="pyrF"
                     /locus_tag="ACHKIK_000333"
     CDS             complement(54606..55343)
                     /gene="pyrF"
                     /locus_tag="ACHKIK_000333"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO9081571.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(55535..56704)
                     /gene="lapB"
                     /locus_tag="ACHKIK_000334"
     CDS             complement(55535..56704)
                     /gene="lapB"
                     /locus_tag="ACHKIK_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO9081572.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(56711..57019)
                     /gene="lapA"
                     /locus_tag="ACHKIK_000335"
     CDS             complement(56711..57019)
                     /gene="lapA"
                     /locus_tag="ACHKIK_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO9081573.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(57168..57932)
                     /gene="pgpB"
                     /locus_tag="ACHKIK_000336"
     CDS             complement(57168..57932)
                     /gene="pgpB"
                     /locus_tag="ACHKIK_000336"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO9081574.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            58102..58692
                     /gene="ribA"
                     /locus_tag="ACHKIK_000337"
     CDS             58102..58692
                     /gene="ribA"
                     /locus_tag="ACHKIK_000337"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO9081575.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(58756..61431)
                     /gene="acnA"
                     /locus_tag="ACHKIK_000338"
     CDS             complement(58756..61431)
                     /gene="acnA"
                     /locus_tag="ACHKIK_000338"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO9081576.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLAAEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKKEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(61595..61690)
                     /gene="ymiC"
                     /locus_tag="ACHKIK_000339"
     CDS             complement(61595..61690)
                     /gene="ymiC"
                     /locus_tag="ACHKIK_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO9081577.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(61804..61971)
                     /gene="yciX"
                     /locus_tag="ACHKIK_000340"
     CDS             complement(61804..61971)
                     /gene="yciX"
                     /locus_tag="ACHKIK_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO9081578.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(61974..62114)
                     /gene="ymiA"
                     /locus_tag="ACHKIK_000341"
     CDS             complement(61974..62114)
                     /gene="ymiA"
                     /locus_tag="ACHKIK_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO9081579.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(62433..63407)
                     /gene="cysB"
                     /locus_tag="ACHKIK_000342"
     CDS             complement(62433..63407)
                     /gene="cysB"
                     /locus_tag="ACHKIK_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO9081580.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(63617..66214)
                     /gene="topA"
                     /locus_tag="ACHKIK_000343"
     CDS             complement(63617..66214)
                     /gene="topA"
                     /locus_tag="ACHKIK_000343"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO9081581.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            66594..66845
                     /gene="yciN"
                     /locus_tag="ACHKIK_000344"
     CDS             66594..66845
                     /gene="yciN"
                     /locus_tag="ACHKIK_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO9081582.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(66881..67930)
                     /gene="sohB"
                     /locus_tag="ACHKIK_000345"
     CDS             complement(66881..67930)
                     /gene="sohB"
                     /locus_tag="ACHKIK_000345"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO9081583.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            68150..68908
                     /gene="yciK"
                     /locus_tag="ACHKIK_000346"
     CDS             68150..68908
                     /gene="yciK"
                     /locus_tag="ACHKIK_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO9081584.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFLLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            68905..69495
                     /gene="cobO"
                     /locus_tag="ACHKIK_000347"
     CDS             68905..69495
                     /gene="cobO"
                     /locus_tag="ACHKIK_000347"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO9081585.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(69535..70407)
                     /gene="rluB"
                     /locus_tag="ACHKIK_000348"
     CDS             complement(69535..70407)
                     /gene="rluB"
                     /locus_tag="ACHKIK_000348"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO9081586.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(70508..71128)
                     /gene="yciO"
                     /locus_tag="ACHKIK_000349"
     CDS             complement(70508..71128)
                     /gene="yciO"
                     /locus_tag="ACHKIK_000349"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO9081587.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDKPVVVREGVGDVKPFL"
     gene            complement(71125..72006)
                     /gene="rnm"
                     /locus_tag="ACHKIK_000350"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(71125..72006)
                     /gene="rnm"
                     /locus_tag="ACHKIK_000350"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO9081588.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            72144..72188
                     /gene="trpL"
                     /locus_tag="ACHKIK_000351"
     CDS             72144..72188
                     /gene="trpL"
                     /locus_tag="ACHKIK_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO9081589.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    72165..72258
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            72280..73842
                     /gene="trpE"
                     /locus_tag="ACHKIK_000352"
     CDS             72280..73842
                     /gene="trpE"
                     /locus_tag="ACHKIK_000352"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO9081590.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGMENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNMLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            73842..75437
                     /gene="trpD"
                     /locus_tag="ACHKIK_000353"
     CDS             73842..75437
                     /gene="trpD"
                     /locus_tag="ACHKIK_000353"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO9081591.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            75438..76799
                     /gene="trpCF"
                     /locus_tag="ACHKIK_000354"
     CDS             75438..76799
                     /gene="trpCF"
                     /locus_tag="ACHKIK_000354"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO9081592.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKSSPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            76811..78004
                     /gene="trpB"
                     /locus_tag="ACHKIK_000355"
     CDS             76811..78004
                     /gene="trpB"
                     /locus_tag="ACHKIK_000355"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO9081593.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            78004..78810
                     /gene="trpA"
                     /locus_tag="ACHKIK_000356"
     CDS             78004..78810
                     /gene="trpA"
                     /locus_tag="ACHKIK_000356"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO9081594.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
BASE COUNT        18998 a        20757 c        19587 g        19554 t
ORIGIN      
        1 ccccaggcgt tatcgatttt atctgtacgt ccctgattgc ggttattctg acgattaagc
       61 tatttttgct gattaatcag tttgaaaaac agcagattaa aaaaggtcgc gatatcacca
      121 gtgcgcggat tatgtcgcgc attatcaaaa tcaccattat tgtggtgctt gttctgcttt
      181 atggcgaaca tttcggcatg agcctttctg gcttgctgac ctttggtggt attggtggtc
      241 tggctgtcgg tatggccggt aaagatattc tgagtaattt cttttccggg atcatgctct
      301 atttcgaccg tcctttcagt attggcgact ggatccgttc accggacaga aatatcgaag
      361 gtacagtagc ggaaattggc tggcgaatta ccaaaattac gacctttgat aatcgtccat
      421 tgtacgtacc gaactcgctg ttttcgtcga tcagcgtaga aaacccagga cgaatgacca
      481 accgccgcat taccacgacc attggtttac gttatgagga tgcggcaaaa gtgggcgtta
      541 ttgtcgaagc cgtacgtgag atgctgaaaa atcacccggc catcgaccag cgacaaacct
      601 tactggttta tttcaaccag tttgccgact cgtcattgaa tattatggtt tattgcttta
      661 ccaaaaccac ggtatgggct gaatggcttg ctgcacagca agacgtttat ttgaagatta
      721 tcgatattgt gcagtcacac ggcgcggact ttgccttccc cagccagacg ctgtatatgg
      781 ataacattac accgccggaa caggggcgct aaatcagaca tcactggcgt tatccacaag
      841 ggtaacgcca gttcccatca catcaatgct tcacaatata catagtccgg ctgtacgcaa
      901 catcttccgg gttattaatg ggataacctt tcagccacgg cttgattaat cgtccattgg
      961 tatattgata aattggtgca atcggtgctt gctccatgag gattttttct gccgcgttgt
     1021 aatcggcatt acgcgcttta acggtatttt ccatcgatgc ctgggccaga actttgtcgt
     1081 atgccggatt gttaaagcgt gaaatatttc ctgaatgcgt tgacgttaat aatgtcagga
     1141 aagtggaggg ttcattataa tcccccaccc atgaggcgcg gataacatca aaattgccgg
     1201 tgttacggct atcgatatag gttttccatt cctgattttg caatttaaca tctacgccaa
     1261 ggttcttttt ccacatcgat gctacagcaa tcgcaatttt ttgatggttt tctgaagtgt
     1321 tatacaaaag cgtcagcttc agcggttttt gcggaccata accagctgcg ctcaacaaag
     1381 tttttgcctg cgcattcagt tcttcctgac tcatttgttc aaacggcgaa ggttccggcg
     1441 taaatccggc ggtaacatct ggtgtaaaat gccacgctgg cttttcgccc gttcccaaca
     1501 ctttttcggt catcaggcgg cgatctatcg tcatacttaa tgccaggcga acgcgctgat
     1561 ctgccgtcgg ccctttttgc gtgttaaacg cataataata ggtcccgagc tgtggcggcg
     1621 tataaacctg ccccggaata tccttcaaca gcttctgata catatttttc gggaaggatt
     1681 cggtgatatc aatatccccc gcgaggtaac gcttagtggc tgcggattcc tgattaattg
     1741 gcaggaaggt cactttttgc agtaccgttt tggcgttatc ccaataatgg gtattcggta
     1801 ccacgaccag tttttcattg actacgcgat ctttaagaac ataagcgcca ttgccgatca
     1861 gatttccggg tttcgtccac tctttaccgc tttctacgtt ggctttttgc accgggaaga
     1921 aggcaaagtt agcggttaaa ttcacaaacc acggcaacgg tttatcaagc tgaattttca
     1981 aagtatgggc atcaactgcg gtgacgccaa gctggtctgg cgtagcttta ccatcaataa
     2041 tcgcctgtgc gttgttgatt cccgccagcg cggcaaacca tgcaaatggc gatagcgttt
     2101 tcgggtccac cagacgttgc cagctgtaga caaaatcttg cgccgttacc ggtgtgccat
     2161 ccgcccattt tgcgttatcg cgcagggtaa aagtccagat acggttgtca ttacttttcc
     2221 actgagtcgc aacgccgggg acaatctccc ctttctcgtt ctgattcacc agtccttcaa
     2281 acagatcgcg aatgacctga atctctggca ggcccacggc tttagcggga tccagcgacg
     2341 caggctcatc tttaatgtgg cgcaccagct cctgcttctc tgccagtact gtgccgctcg
     2401 gaacttctgc agcatacgaa agagaaatgc tgctgaccaa cagcgcacaa cacgtgactg
     2461 aaacagagtg cttcataagt ttccctctaa ccgacattaa aattgatgcg taattatttg
     2521 tttcgcctta aagaaatgca aacaacttcc atcaaaaaag tgacacagga agcataaaat
     2581 gttttatgta tcaatatatt aaatcagatt tagtgtaaat aatcgacgcc aaaatgcggt
     2641 aaagcacata gaacaccgaa cactacgttc aggcttcgca cgttcaaacc agttttcggt
     2701 atcataatca gcatctgtcg ccattgtacg aagaataaaa aataaccaaa acgttgaaag
     2761 gaactgagaa tacgttaaga gagaaattaa attaatgtat cagcaagtag tgctaaacag
     2821 cacctttata cttcagccta tctataaata atgagaatgc cgagccagca tgtcgacggt
     2881 gtggatagta caggtgatga ccgggtaaat ctggtgtgaa tttatccaaa acacgtatca
     2941 gttttttttc tttaatagcc cgctcaacct gatcataagg tagatacgcc aatccatgcc
     3001 catcaattgc agcatcaatg atcaggtcta tcgtattcag taaaagctga ccttccatgc
     3061 gaacacgaac ttcacgtcca ccgcgtatta atctccagcg atttgctgta cccgatgtgg
     3121 gaagatacaa attaattgcc tgatgatcta ttaattgtga cactgacgtt ggaacacttc
     3181 ggcgagaaaa ataatccggt gagccaacaa tagccattgg tatatctggc ccgattcgaa
     3241 tggcgatcat atctttatcc atctccccac ccagacggac gcctgcatca aaacgttcag
     3301 aaacgacatc ggtcaaacca taatcaatgg tgagctgaat atcaatttca ggatgcgatt
     3361 tcaggaatgt gcgcattgct ggtaacaata tcgtttttgc tgcatgttct acagtagtaa
     3421 tacgtattgt cccggatggg cggttctgca gctcgctcag ggatgccagg gctgaatcta
     3481 tgtcatgcaa catagggcca agaacagaca aaagatgctc gcccgcttca gttggaacaa
     3541 cgctgcgcgt ggttcgcgtc agaagccgca atcccaatcg ttcttctata cgacgcacta
     3601 tctggcttaa agctgactgc gccatgctca ggcgggctgc tgcacgagtg aagctacgct
     3661 cctctgcaac gacgacaaac gccatcagat cagcgatttc ttcacgcttc attcagtatc
     3721 tccatgcaat ctattaatca tttaaatgat aggtctaatc atgattttgg gtctaatcaa
     3781 cttaatgatc aatatctata gtgatatcac caaccgttaa aacgaaccga caactccatg
     3841 aggaacggaa tgatgaataa taaagtcagc ttcactaaca gcaataatcc aaccatctct
     3901 ttgtctgcag tgagctattt ccctccaaaa tttgatgaaa cccgtcagta tcaggcaatt
     3961 gtggtatctc acccaggtgg gggcgttaaa gaacaaacgg ccggaaccta tgccaaaaaa
     4021 ctggcggaaa agggatttgt cactattgct tatgacgcat cttatcaagg tgaaagtggc
     4081 ggcgagccgc gtcagttaga aaacccatac attcgtactg aagacatcag tgccgtgatt
     4141 gattacctga caacgctttc ttatgtcgac aatacccgaa ttggtgcgat gggaatttgt
     4201 gccggtgcag gatatacagc caatgctgca attcaggatc gccgcatcaa ggccatcggt
     4261 accgttagtg cggtcaatat tggttcgata ttccgtaatg gttgggaaaa caatgtgaaa
     4321 tctattgatg ccctgccata cgttgaagcg ggttcaaacg ccagaaccag cgatattagc
     4381 agcggagaat acgccattat gccactggca ccaatgaagg agtccgatgc gccgaacgaa
     4441 gaactgcgtc aggcctggga gtattaccac acccctcgcg cgcagtatcc aacagcaccg
     4501 ggttacgcta ctttgcgcag ccttaaccag attattacct atgatgctta ccatatggcg
     4561 gaagtgtacc tgactcagcc gacgcagatt gtggcgggca gccaggcagg gagcaaatgg
     4621 atgagtgacg acctgtacga tcgagcatca agccaggata aacgctatca cattgttgaa
     4681 ggcgcaaacc atatggatct ttatgacggc aaagcctatg tggctgaagc aatttcagta
     4741 ttagctccgt tctttgagga aactctgtaa tgaaacatat tcaaatccgc aatagtgata
     4801 tggcctggca tattgctgcc aatattcagt ttccaccaaa ttttgatgag tcaaaacagt
     4861 atccggcgat tatcagcgtc catccttttg gcagttgtaa ggagcagacg tctggcaata
     4921 tctatggcaa agcattggct gaaaaaggct atctcgttct cgcttatgac gccagtttcc
     4981 agggagaatc tggtggggag cctcgctgga tagaagatcc gacacagcgc gtagaggata
     5041 ttagccgtgt tatcgattat gcagtaactc tgccatatgt taatgctgaa cgtattggcg
     5101 tgctgggagt gtgtgggggt ggtggctatg ccattaatgc cgcgctgaca gaaaaacgaa
     5161 ttaaagcagt agtgagtatt acgggtgtta atatcgggcg tctttttcgg gaaggtttca
     5221 gcaattacga tccgattggc gcattgaatg caatggcgtc gcaacgaacc aaagaggctc
     5281 gtggtggaga attacagatc aacgaattac ttcccgccag cctggatgca gcaaaagccc
     5341 atggactgac cgaacgcgat gtttatgaag caactgacta ctataaaaca ccacgcgggc
     5401 aacaaccggg aggtgcgaca aaaatgttgt tctcacatgc acagaaaacg ctggcctggg
     5461 atgccttcgc atttactgaa gtgttactga ctcagccggt gatggtagta gttggtgaaa
     5521 aagtcggtgc cttcggtgct tatcgtgatg gactggaagt ctatggccgg gcgatggttt
     5581 cgcaggatcg tcagttagtt tcattacccg atttctcaca ttatgaactg tatgacaaac
     5641 cggaagcagt tcagaaggcg ctggaaaaag ttattccttt tttcaacacc catctgggat
     5701 aagtttcatg agaaatatta tgaaaaatgt actgattctt ggtgctggcg gtcagatagc
     5761 ccgccatgtg attaatcaac tggcagataa acaaaccatt aaacaaactt tatttgcccg
     5821 tcagccagca aaaatccaca aaccctatcc aactaatagc aagattatta tgggagatgt
     5881 actgaatcat gcagcactga agcaggccat gcagggacag gacgttgtgt atgccaacct
     5941 tacgggggaa gatttagata ttcaggcaaa ttcagtgatc gctgcgatga aagcctgtga
     6001 tgttaaacgt ctgatctttg ttctttccct cggtatttat gatgaagttc caggaaaatt
     6061 tggcgaatgg aataatgcag ttattggtga gcccctaaaa ccattccgca gggctgcgga
     6121 tgcaattgag gcttctggac tggaatatac aattcttcgc cctgcctggc tgactgatga
     6181 agatattatt gattatgagc ttacctcacg caatgaacca ttcaaaggta cgatcgtatc
     6241 acgtaagagt gtctcagcat taatcacaga tatcatcgat aaacctgaga aacacattgg
     6301 tgagaatata ggtatcaatc aacccgtgac tgatggtgac aagccattat tcatgtaaat
     6361 caattacata gttaccatcg taatgtacta tgtgcagtac gatttattaa acttattaag
     6421 gtgaactgat caatccgatg cgattaacat gtgtagaacc agtaagaata aaatacgcca
     6481 tgtatataac attacgactt acatctaagt cagcataatt ctaatatctg cacatcaata
     6541 aaaatgcaaa caacttccgg caaaaaagtg atacaagatc gatgacatag cgttgaagaa
     6601 aaggatttga taatccgcaa aatttgcaca acactgcctg taatacttcc tgaaccagag
     6661 acgatcatga ccgtaaaccg cccacgcgcc gaacgcggcg catttccgcc cggaacagaa
     6721 cattacggac gttcattatt gggcgcgccg ttaatctggt ttccggccac tgcagccagt
     6781 cgcgaaagtg gtttgattct ggctggcact cacggtgatg aaaactcttc ggtcgtcacg
     6841 ctctcctgtg ctctgcggac tttgacacct tctttgcgcc gccatcatgt agtgctgtgt
     6901 gtgaatcctg acggctgcca gctggggtta cgggccaatg ctaatggtgt ggatttaaac
     6961 cgaaactttc cggcggcgaa ctggaaggaa ggtgaaacag tttatcgctg gaacagcgcc
     7021 gctgaagaac gtgatgtcgt attgctgaca ggtgacaaac ctggctctga acctgaaacc
     7081 caggcgctgt gccagcttat acaccgcatt caacccgcct gggtggtctc attccacgat
     7141 ccgctggcct gtattgaaga tcccagacac agcgaattag gcgaatggct ggcccaggcg
     7201 tttgaattgc ctctggtaac cagcgtcggt tatgaaacac ctggctcttt cggtagttgg
     7261 tgtgcggatc tcaacctgca ttgcatcacc gcagagtttc cgcccatctc ctccgacgaa
     7321 gccagcgaaa aatatctctt tgcgatggca aacttgctgc gctggcatcc taaagatgca
     7381 attcgcccgt cgtgaactga agcgccggtt ccacatctac cgccagccag gtcggtccgt
     7441 caagatcgac gaaactgacc tgcggcacca gcggtaaagc ggcgctaatg gcccgagagg
     7501 tacacaacat gcagcccagc atcagacgaa aaccttgtgc acgcgcttca gtcgccagcg
     7561 ccagcgcttc cgtcagacca ccggttttat caagcttaat gttaaccatc tcatagcgcc
     7621 ctttcagcgc cttcagattg ctacgtgtat gacaactttc atctgcacaa atcggtaacg
     7681 gatgaataaa attctccagc gccgcatcgt cctgcgcagg aagcggttgt tcaagcatcg
     7741 caacgcctaa atccgccaat agctggcaac gcgccgccaa cccttctgca cgccaggatt
     7801 catttgcgtc aacgatcagc gtcgcatcgg gcacagctgt gcgaattgcc accatccgct
     7861 cactgataag atggttatcc agcttcactt tcagtaattt cgcgcctgcc tgccagagtg
     7921 ttgatgcact attggccatc tgatcaggcg taccgatgac taccgtctgt gcagtggtga
     7981 ctgtcccggg aagcgttatg ccgatcaaat cagccagcga ttgctgctgt tttcgcgcgg
     8041 ccagatccca caatgcacaa tccagcgcat tacgtgctgc gccggcaggg agaatttttt
     8101 gcaactcctc ccgtgtcagc cctttctcta attgcggcac gacgctcata atttgcgcca
     8161 ttaccgaggc atcactttcc ccataacgcg gatacggcgt gcattcgccg gtgcctttaa
     8221 taccctcttc ttccagttca accaccacca cgcgcgcttc actgcgactt ccccgggcaa
     8281 tcacaaacgg ggtatgtaag ggccaggctt cctcaaatac cttaacggtt ctcatcttta
     8341 ctccttgttg tcgcgatggt ctgcggatta agcgcaaata gcgtttgctg tgttattgac
     8401 agttagcata aactaggtgt gacgttaact atatgtaaac aggaaagata atcatgtcac
     8461 aaaccgttca tttccagggc aacccggtta cagtcgccaa ttccatcccg caggcgggta
     8521 gcaaagcgca gacttttact ctcgtggcaa aagatctgtc tgacgtcacc ctcggtcagt
     8581 ttgcgggtaa acgcaaagtg ctgaacattt tcccgagtat tgataccggt gtttgcgccg
     8641 catcagtacg taagtttaac caactggcaa ctgagatcga caacaccgtt gtgctgtgta
     8701 tctctgccga tctgccgttc gcccagtctc gtttctgcgg cgcagaaggt ctgaacaacg
     8761 ttatcaccct ctccactttc cgtaacgctg aatttctgca agccttcggt gtggcaattg
     8821 ctgatggccc actgaaaggt ctggcagcgc gtgccgttgt ggttattgac gaaaatgaca
     8881 atgtgatttt cagccagctg gtggatgaaa tcaccaccga gccggattac gaagcagctc
     8941 tggctgtact gaaagcataa gttaatatgc ctgatggtgt tgcaccatca ggcatattcg
     9001 cgcttactct tcgttcttct tctgactcag accatattcc cgcaacttat tggcaatcgc
     9061 ggtatgtgaa acgccgagac gttttgccag tttgcgcgtg ctgggataat tgcgataaag
     9121 ctgggttaat accgagcgtt caaaacggct ggtgatttcg tccagcgaac cttccatcgc
     9181 atcttcgccc acggctaccg ttgcggcgtc ataatccggc aacaaaatat cctgtggacg
     9241 cagctcataa ccgtccagtt gtgtcagtgc gcgatagata gcgttcttta actgccgcac
     9301 atttcccggc cacgcataac gcgtaagtac agtattcagg tcagcggcca gtttcggacg
     9361 cggcacgccc tgctcgtcgg caaagcgggc gacgaacagc tcagttaacg gcatgatgtc
     9421 ctgcggacag tcacgtagcg gcggcagatt gagcgtcaac acgttcagac gataatagag
     9481 atcttcacgg aacatgcctt tttgcaccag ttcgaccaga ttcttctgcg tagcgcaaat
     9541 cacccgcaca tcgacatgca cctcatggtc ttcgccaacc cgacggaaag tgccatcatt
     9601 aaggaaacgc agtaatttcg cctgcatccg tggtgacatt tcccctattt catccaacag
     9661 caccgaacca ccgttcgcct gctcaaagaa tcctttcttc ccttccggag catgaccaaa
     9721 cagttcactc tcgaccgcat cttccggtat agacgcacag ttcagcgcca ggtaaggttt
     9781 gcccgctctg gggcttgcct gatggcaggc gtaggcaaag agatctttac ctgtacctgt
     9841 gtcacccgta atcagcagcg gcgcgcttag catcgccagt ttctgcgcct gttcgacaac
     9901 atgcttcatt ttcgggctga cggcgacaat ttgactgaag gcgctgacgt cctgggcggc
     9961 gacattttgc aactggcggc ccatacgaat cgttgatcgc aacatcacca ccgcaccggt
    10021 caggacgtgt tgatcatttt catcctgaag ataaacaggc gtaatctcca tcaggaaatt
    10081 ctgcccatta ataacgacat gctcgttatg ggaatcttgc ggttcgcttt ccagccaacg
    10141 taaaaaatta aagccgttaa tcaattgtgc ggcggtatgg ttgcgcaggc gatccaattt
    10201 ttgcgcaaaa agctgacagc tcgccgggtt cgccatatcc actttgcttt tcatatcgac
    10261 agagagcaca ggttcgggca acgcctccag taacgcgctc aacgccagat gctcacgttc
    10321 ggaaggcatc cacgggacag tacgcacatc ggtaacacct gcaatacggc gtatttcggc
    10381 catcagactg ctgaaactct caaactccag ttcagcaaaa ttgaggtaga ttcgcccaat
    10441 gggatcaatc tcaataccgc gtaaatcaat gcctcttagc acgagtagat cgagtaattc
    10501 gcgggtcaga ccgagtcggt cttcacaaaa gacttccaga cgcatgggaa ccttcacctg
    10561 aaaaaagaac aattaatatg atgatatgac actatttgga tagcaggaag gtttctgtca
    10621 acaatcattg acacaaagac ggaaaaacgg gcacttcggc gtaaagatat ccggtcagca
    10681 cccgatattg cattattttt cgctgggcgt tttgcctttt tgcagcgttt ctttcacctg
    10741 accgataagc tgacgacgga aatccccgag gcgaggtttg tcatcgtcaa tccacggcag
    10801 cgggcggcaa agctccatag ctttaatccc gagtcgtgcc gtcagaagtc cagcaccaat
    10861 tccctgagct gcgcgggtag acaaacgagc agcgagatct tgcgacatcc agtccatccc
    10921 cacttcgcgc accagttcgc tggctccggc aaaagcgata ttcagcaata ccagcttaaa
    10981 cagacgcaaa cggctgtaat accccagttc aatgccatac agcgtggcga tgcgattaat
    11041 taaacgcaga ttgcgccagg cgataaacgc catatcgacc aacgccagcg ggctgaccgc
    11101 aatcatcaac gttgattccg ccgccgaacg gctgatttcg cgccgcgcct gggcatctaa
    11161 aactggctgg accaaatgcg catacaaact gaccacttca cggtcgtttt gcgtttcatg
    11221 gattgaggca taccagcgtt gcagcgctgg atgagactga tcaatacccg cctgctgcgc
    11281 cagtttttcg caaaatgcgc ggcctttgcc cgtgccgtgg ctgtgcaaca aatcgcgcgc
    11341 ttcgtcgcgt tcatgggcgc gctgtcgcaa gcgccataag cgccgccact ctgttaccac
    11401 agaacctacg ccagcgccga taatcaatgc cccagcggca catccaccca gcgccaccca
    11461 gtcctgagtt tgccaggcat tcattgtcca ctgtacaccc tgcccgacaa cgcttgcgcc
    11521 aaacagagcc agcccgccca tcaccatttt gcgccacagg ctgcgtttcg gacgtaacgc
    11581 tgcatccatt accgcttcaa cttgcccctc ttcttcaggc gcttcatcga gcgtggccgg
    11641 ggcaaaattt tgcgcctgat tttcgtcaaa ggtctgctgc gccctgaatt ttggattctg
    11701 atcgacctcc agcggaccgt cgaaatcaat acgtggtttt aacggttcgg tcatcgcaat
    11761 ttatctccta ttaaaaattc aagcgcggca tcaagacgaa tatgcggcag cggtttgtcg
    11821 acatccatca cctgcgggcg aaacgcttca aactggaacc cttgcttgtc ccagaacgcc
    11881 tgaccgggca aacgcgcagg aacttcaccg ggataaacgg tgagtggcgc accatcgctg
    11941 agccggttac cgcgcaatgc cggaattttc tcgccgttga cgtcaataat gccgctggtg
    12001 gtcgcctgca ccgaggccag ccccaggcaa tccatactaa tcccttcaaa cgccgcattt
    12061 tgccaggcat cctgaatcag ttgttgcagt aatgaaacca tattggcgtg ctgatcgatg
    12121 gtcacatggt ccgctttagt ggcagcaaac aatagcttat cgataaccgg cgaaaacaaa
    12181 cgccggaaca aggtacgctg cccgtagtgg aaactttgca tcagctgcgt cagtgccaga
    12241 cgcatatcat taaatgcctg tggcccactg ttgagaggtt gcaggcaatc aaccagcaca
    12301 atctggcggt caaagcgcag aaaatgattc ttatagaacc ccttcaccac cttctcgcag
    12361 taataattaa accgctcgcg cagcattccg gcattggtgt gcttatcggc ctgcgccagt
    12421 ttggactcgc cccaggcatc gacatccggc cacgggaaga attgcagcgc gggcgcacct
    12481 gccatatctc ccggcaagac aaagcgccca ggctgaataa agtgcagccc ctgctgttta
    12541 cagtggtgga gataatcggt ccacgcggcg gcaatgtctg ccagccggtt ttcgtcggca
    12601 ggcgctagcg ggtccagccc ttcgcacatc attcgccatt tgaccgacca ttcgccgcgc
    12661 tgaccattga gtaagcccgt catctggcgt gaccagctta aatagtcctg cgccagcatc
    12721 ggcaggtcga gcaaccattc gccgggataa tccacaatct ccagatacag cgtggaggtg
    12781 tccttgaagt ggcgtagcag cgaatcgttc gatttaaaac gtagcgccag gcggatttca
    12841 ctgacgccgc gcgttggcgt cggccaggcg ggaggatcgc catataactg cgccagtcct
    12901 tcatcatatg tgaagcgcgg aatgccaaag tcacgctgag gaatgcgttt tacgcccagc
    12961 aggcgctctt cacgcaccgc gcttaacagc ggcaagcgtg ctccggcgtg aatattgagc
    13021 aactgattga ccatcgcagt gataaacgct gttttgccgc tgcggcttag tccggttacg
    13081 gcgaggcgca gatgtctgtc gacaccccga ttcaccagcg cattaagttc atttttaagt
    13141 cgcttcattg ctgtcctgtg ctgctctgaa gaccagaaaa aaatcattcg taaggatatg
    13201 aatgtggggg ccaggccatt gattatcaac tgcccgtgtt gtcatcgacg ggcgaactcc
    13261 ataagatgtg cagcattcac gttcggacgg agttcctgac ctatgtcccc tactatttat
    13321 gatattgcca gggttgcagg cgtatcaaaa tccaccgtat cacgcgtgct gaataagcaa
    13381 actaatatct ccccggaagc gcgcgaaaaa gtgttacggg ccattgaaga attacagtat
    13441 cagccaaaca agctggcccg cgcgctgacc tcttcgggtt ttgatgccat tatggtgatt
    13501 tctacccgtt cgaccaaaac tacggcgggt aatccgtttt tctctgaagt tttgcatgcc
    13561 atcaccgcca aagctgaaga ggaaggtttc gacgtgatat tgcagacgtc gcacaatccg
    13621 gcagaagact tacaaaaatg tgaaagcaaa attaagcaga aaatgattaa aggcattatt
    13681 atgctaagtt cgccagcgga tgagtcattt tttgcccaac ttgataaata cgatattcct
    13741 gtcgtggtga ttggcaaagc tgaaggtcaa tatgcccatg tttattctgt cgataccgat
    13801 aattttggcg acagcattgc gttgaccgat gcattaattg aaagtgggca tcaaaatatt
    13861 gcctgcctgc atgctccgct tgatgtccat gtttcagtgg atcgggtaaa tggttataag
    13921 cagagcctgg ctgcgcataa tattgcagtg cgtgatgaat ggattgttga cggcggttat
    13981 acccatgaaa cagcactgca agccgcgcgg gaattattaa gccagtcacc gttgcctgag
    14041 gcagtgtttg ctactgacag cctgaaatta atgagtattt atcgtgcggc agcagagaaa
    14101 aatattgcta ttccgcagca gttagcggtg gtgggttata gtaatgaaac gctgtcgttt
    14161 attttaacgc ctgcgccagg cggtattgat atcccaacac aggagttagg tcaacaaagc
    14221 tgtgagttat tattccgctt aatttctgga aaaccgtcac cacagaatat taccgttgcc
    14281 acgcatatga cgctgaaata aataccagct accttgcctg aatgccggat gcgatgctag
    14341 cgcatcttat ccggcctaca tttatagttc ggataaagta ctcgtgccgc atccggcaat
    14401 ttagcccatt atcagaacga gtaatttacg ccgacacctg cataatggaa tttatcgctg
    14461 tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca gcgaaacgga aagcccgttc
    14521 tggaaatcat aaccgtaaaa taaacctaca cggttaaaat catggccttc acgttcaata
    14581 tcatcctgcc agtcccagtt actccagcga tccagcccaa tgcgcgtata cggcgtcacc
    14641 gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt cttgcgtaga aaactcaccg
    14701 ttattgcggc tgtcgtccat attaaagccg cgctcgagat aatagttcac tcgcaaggca
    14761 accgtttcgt tgaaggtata ttgcaggcct gtttccgttt cgactcgggt atcagcgtaa
    14821 ccggtcgtgt tcagatcgtt ggcaaattta tacatcgaca accaaccgtt gaaacgtaaa
    14881 tcgtcagtca gtttcacatc ccagtctggc gcgattttcc agcgctgcat attcgccgtg
    14941 tctttacccg gttcatcaac gtagtgataa ccataattac ggaaaccgcc ggtcaggccg
    15001 aaactgaaat catcgttttc gaggaactga taatgcacct ccagctccgg gcgatcaaac
    15061 cacgttccac gtttacccgc gctataatct accggccctt cctgataata ggccagagca
    15121 attctccacg gcccgttggc ggcattaaaa tagactgaag gctccgccag cccatccata
    15181 tcttcgccat aaccctcgac gttttctatt tcgtacatcg cgccgatatt aaagtgccag
    15241 tcgttccttt cctcggcctg tgcgcaggcc attcccgcac acatcaccag tgcggtacag
    15301 ggtaataact ttttcattat tgttccttgt attattctgg gggaatgcct tccccctgta
    15361 tttagcgaat tgctatttcc gtttctgcat caaagaaatg acatttcgtc atatcaaaat
    15421 gaatagtgat attttctcct gcatgataat catttaacgc accagcacgg accactaact
    15481 cgtgcccccc aaccgtggtg tagagcataa attccgcacc ggttaattct gccacgctaa
    15541 ttttggcgga aatgttattt tcctcttgcg cgtccggatg aatatcttcc ggacgtattc
    15601 ccatcacgat gggcttatgc aaacttccct gtgttttcag aaccgctaat ttctcttcgg
    15661 gaatggttaa tttaagcgtt tccgtaacga atttatcgcc atcgatcgtg ccacgaataa
    15721 aattcatcgc cggtgatcca ataaatccgg caacaaacat attcgctggt tggttataaa
    15781 ccgttttcgg cgcaccaact tgctgaacaa tcccatcctt cataatcaca atccgcgtcg
    15841 ccatggtcat cgcttcggtc tgatcgtggg tcacgtagat catggtggta ttcagcttct
    15901 ggtgtagctt gctgatctct gcgcgcattt gcacgcgcag cttggcatca aggttagaga
    15961 gcggttcatc cattaaaaac acgcccgctt cgcgcacaat cgcccgcccg agcgccacac
    16021 gctggcgttg cccgccggaa agcgcgcccg gcttgcgttt caggtactca cgcaggccga
    16081 gaatttgcgc cgcccagttc acccgctcat caatcacctc tttggcgatt ttttgcatct
    16141 tcaggccaaa cgccatgttg tcgtaaaccg tcatatgcgg atacaacgcg tagttctgga
    16201 acaccatcgc tatattgcgt gctttggctg gaacgtcatt cattcgtttg ccgtcgatca
    16261 acagatcgcc gccgctgatc tcctcaagcc cggcaatcat gcgcagggtg gtcgacttac
    16321 cgcagcccga cgggccgaca aacacgatga actctttatc ggcaatttcc aggttgaagt
    16381 ccttcaccac atgcacctgg ttatcgtaga ttttttgaat atgttgtaac gaaagctgag
    16441 ccatgttgat tcctttgcta tacgttttgc cagaaggccg ataaccgcga ccaggtcaga
    16501 gagtccgttg aaggcaacag taattgcgcc ccggttaagc ccgcgccgat ccccaccgag
    16561 cgcataccgc tggcgttaat ggcgtcaatg cccgcctgcg catcttcaat gccgatacat
    16621 gcctgcggcg gcacgcccag cccggcacag gcggcgagaa agatttccgg gtccggtttc
    16681 gagtttttaa gttgggaagc atccgcgcag aaggtgaaaa actcgcgcag ctccagcgcc
    16741 gctaaaatcg tcggcgcatt cagggagaca gaagccagcc caaccgggat ctgctgtgca
    16801 cggagatctg ccagcaaaga gcgaatgccg ggtagaacag cgctgaccgt caactcgcgc
    16861 agtgagtgga catagagcag atttttgcga tacgccagtt gcgccctctc ctgcgagtta
    16921 aagtcgccct ctttgccccc gtgttgcaga atgcgccgca gagactcatc gcggctgatc
    16981 ccttttaggg attcgttaaa ctgcgcatca atgctgatgc caatttcagc ggcaatctgc
    17041 tgccacgcct ggaaatgcag atgcgcggta tcggtgatta caccatccag atcgaaaatt
    17101 accccttgca gtttcattca tcctcctgat gcgtggtagc ggtcccattg aagggcaaaa
    17161 taaaatcacc caaacagaaa acagattctt cagccacgga tataagctga ccgttcaaac
    17221 gcagtgaaac gggcgcagaa gttcgaatcg caatacgctg cgcgtcaaga gtgacctgta
    17281 attcgcagcc ctgccagaac agagggaaag acaactgttg ccactgctca ggtaacgccg
    17341 gattgagatg caattcaccg tcacgcacgc tcaccccggc aaaaccctga atcgccccca
    17401 gccagatagc gccagttgcg gcagcatgga taccatcatc acaactatgc ggatcagcac
    17461 caagatcgat ttcagtcccc tcgcgccaga actgataact ttgggtcagc aggccacagc
    17521 gtgcggcaac aatgccgtgg attgctttac ttaatgacga gtcgtgaata gtgcgcggtt
    17581 cataaaattg cagattggca agacacgatg ccgctgagaa ctgctccggc agcatgtaat
    17641 tgagcatcac cacatcagct tgtttgagga tttgcatctc gttcacttct gcgcgtgaat
    17701 aatccagcag aatggtttgc ttccccgccg ccgctttgta tttcgccaga ttaatcgccg
    17761 gcttagccat aaacgaatca tcctgcggca aaacgccgtc gggctgaatt tctggcagcc
    17821 gcagttcttt aaggaacatt tcggcgcgat ggataaacgc atcgtcgcta cagccaaact
    17881 ggcgggcaat actcagcgcc tgctggacgt tgtaatacgc catatagctg gtgaaggcgt
    17941 tattattgac atgttcggta tattcgtcag gcccaataac atcatgaatt tccagacggt
    18001 cgttaaccct caccgcgcgg ctaatccaga actttgcagt ttccagaagt agcgccatgc
    18061 cttcatgcgc aatgaaactt tcatcccccg tggtctgcca gtattgaata accgcccagg
    18121 cgatatcggc caccagatga tgttccgcct gcgccgaggc cactttttgc cgcagcccgg
    18181 tgcgaatgtt aatggcggca aattccggcg tctcttcttc gccgctgcgc gcgctttccc
    18241 acggaaatag cgcgccctgc cagccgttgc gtcgcgcttt ctcctgcgcg cctggcaagt
    18301 tgtgccagcg ataacgcagt aaacttcggg caaccgtcgg atcgctaaac agatgaaacg
    18361 gtaacaaaaa tacttctgta tcccagaaaa cgtggccttt gtagccttcg ccggttaagc
    18421 cttttgccgc aatgctgctg cgctcgtcgt gggcaggcgt catgatgcgc agatgataaa
    18481 gcgcataatc taacgcttgc tgatcgtgcg cttcgccgcc atttaccgtg atacgacgtt
    18541 tctgccacca ttgacgccag ttttctgttg atactgcaag aagttggtcg taactctgct
    18601 gcgcgcacat ttcaagctgg cgaagcgacg cgctgcccca ctcgtctaaa acggcttgcc
    18661 tgtcatcccg ccagtcgatc cacaccagtt tttgcaacgt cactgtctcg cctgcatgaa
    18721 gctgcgcact ggtatgttgc tgcaaacggc gctctttggc ggtatagcat tgctgcacat
    18781 caccgctcac catacagcaa cagctgatgg ccacatcact gcgtccatcc tgggtggtgt
    18841 agatcccctg catcagatgc tgaccaaaca cccgcacctg ggtttcgtcg agatgttggc
    18901 gaccgtggtt ggtttgcgtg gcgtcgatac ctgttgaaat cagcactgac gcgtcggcgt
    18961 ccagtggcgt aatagtgatt tccagcacaa tgagcggcag ttggtctgcc gaaacaaagc
    19021 gacggctggc gatggtgtaa cctgtgccgt tgctggtacg ccagacaacg ctacggcgta
    19081 attctccact ggcaaagtca agttcacgct gccaggcttc gcgggataac gagaaaacct
    19141 caccatttat ggcaatctcc atccccaaga tatcaggcag gttcaccagt tcgttgattt
    19201 caccttttcc cgcccgatga tacagccccg ccagatacat ccctcgcgtc tggcgcgtgt
    19261 aatcttcttc atggctggcg cgaagcccaa gatagccgtt cccctgcgcc atcagcgatg
    19321 catacttgtt cagggtatgc tggctgaaat ggggttctga taacgttact ggcctggtca
    19381 taattccaca cgcgtccctg tttctgcgga ttgatacaac gccgccacca gttgctggat
    19441 gatgtacccc tgctcggcgt cggctatcat cacgggcttg ccctgtacgt gattgataaa
    19501 ggcttccatg ctgcgcaaat ggcggttgtc gtctgccagt tcccgttgca tcagcgtcat
    19561 taattcaccg ttgttatcgg tgtagatatg tgctggaaac agcgtcgcac cggctttatc
    19621 accacaaaag ctgacgttca taatcgactg ttcgcggatg ttgagtgcaa atgacgtttc
    19681 cagccacaga atgccgccgt tatgaaattc aatggtgcca aataacgaat cttcgacgct
    19741 gtaagttgcc ggatcccact cgccaaattg accgcagctc ttttgcgtgc cgatcttttg
    19801 aaagctatgc gcattcacgc ttttcaccgc cggaaagccc agcacataca tcgccgcatc
    19861 cagcatatga atgccgatgt cgataagcgg gccaccaccc tgcagttctt tattggtaaa
    19921 gacaccccag ccgggaacgc cgcagcgacg cagggcgcgg gcggtggtaa cgtaaatctc
    19981 tcccaaaacg ccgttggtca cctgttcacg cagctgttgc gtatcgagcg caaaacggtg
    20041 gtgaaagtcg taggccagca ccttgcccag tttgcgcgcg gtatcgcaca tttcccgcgc
    20101 ctgttctggc gtcatggcgg gcggtttttc gcacatcaca tggcagcccg cttccagtgc
    20161 catcagggta tgttcgtaat gaaaacggtt aggtgagcag acgctaacca catcaggttt
    20221 caccgccagc agcatggcct gcggatcgtc ccacacggat gcattaccgt atttatctgc
    20281 cagcgcctgc gcctgggaaa ggcggctgtc acagacagcc accagttcca gatcgttgcg
    20341 ggtgcagtag tacgaagcat gaaccttatc cgccacctgg cctgcgccta ttatcgcgac
    20401 ccgcagcgga gagcttgtca ttgcactttt cacaaagacc tcttagcagg tacgcaacca
    20461 ggccaacgaa tcacggtacg cctgggcagg ctcttccgcc cggatacgcc cttcgtacac
    20521 tacatagccc tgatagttat ccgcgcgcag ctgttcaaac agcgcgtgga aatccagggt
    20581 gccgctgccc ggctggtagc gatggttatc cgcaatatgc acatgaccga gcaggtcgcg
    20641 gttgtcatgc agcgcctgcg ccaggttatc ttcttcgata ttcatgtgat agaaatcgcc
    20701 gataatctgt acatgtttaa gatcgttttc gacgatgtaa cggcgggcat cggcgagggt
    20761 gttgatcata tgatcctgat agcggtttaa cggttcgaga tacaccacgg ttccggtacg
    20821 cgcggcgacc tgttccagta cgtgcaggga atcactcacc attttgcggt cgccgtccag
    20881 gctacgcggc gaggtcatcg gcggtaagcg gaaggtaaac atgccccacg ctgccggaac
    20941 gacgatacct ttacccccca cttctgccag cgcttcgaga atgcgttcga tctgctttaa
    21001 gccattaaga cgacgctctt cgataaagtc gccaatccaa ccgtcatagc caccgcaggc
    21061 ggtggtcacc ggtaaaccgg tttctttgat tgctgctttg acttcttcga gattgttgac
    21121 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaattt
    21181 ctccagaatg ttttccggaa agaatgcctg attctgtgtg cctattttca ttttttcatt
    21241 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    21301 tgcatgtagc tttccgggct cttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    21361 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    21421 cgcggataat ccgggttcgg ttcgctgcac gcacgggaga aaacaatttt ggcgttattg
    21481 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacataa
    21541 gagatggtgc cgccataagc aaggccacgc agcgccgatt gcagcgcgtc ggcgtaaccg
    21601 ctggtttcga taatcacatc ggcaccctgc ttgccggtca gcgttttgat ctcttttcct
    21661 acgtcagtgc caatgaggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    21721 cgataggcga taggatcgac gccaatcacc accgacgcgc cagcgcgttt cgccagttgg
    21781 atggctattt gaccaatcgc gccaagccct accaccacca caaaatcacc cacgcgcacg
    21841 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacaaac ggcgtttttc
    21901 cacgagctgc cttccggcat tttgcgcagc ttgtagttat tcaccgcgtt aatgatgacc
    21961 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc ggtaacgtcg
    22021 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    22081 atgccgcgcg gcgcatctgc cggacgcgga gtgaacatct gccattcgcc gttaaagtct
    22141 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    22201 ccgaaacgca cgcggatttt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    22261 agcgcagcaa cacgcggtgc tgtggctact aactttttca tttaaaactc cttgtaaaga
    22321 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    22381 gaaagcgcat acataatcac caccggcaat gcggtcacca gtgaggccgc catcatccgt
    22441 ccccagatgt aatctggcgt actgaacagc gcgttcaggc ccaccggtaa ggtgaaattg
    22501 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    22561 tagacgaaca cggatatcag cccggacatc gccaacggca cggtgatgcg gaagataatt
    22621 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcggg gatggtgtcg
    22681 aagtagcttt tcagcataaa cacggcggtg ggcagagttt gcgtcaccat ggtgatgatc
    22741 agcgccatct cggtgtcgta aatgcccagc gcggtgatga ttttgaacag cggcaccacc
    22801 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cattgatggt catccgacct
    22861 ttaaagcgca ggcgggaaag cgcgtaagcc ccaagaatgc cgaggaatac cgccaccacg
    22921 gatgaaacca ccgacaccac cagactgtta cggaagtagt cgacaaacgg gaaaatcatc
    22981 gggttaaaaa tgtcgacgta atgctccagc gtccactgct gcggcagcag cgtaggatgc
    23041 agtgagatcg cctctttcgc gcccttgaac gaggtcatca gcatcacaaa aaatggaaac
    23101 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatgcgact gagtgtgcgt
    23161 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    23221 gatgaagagc accaccgaga tcgccgccgc ttttcctaaa tcattaaagg caaaggcggt
    23281 tttgtagaga tatacaccga gaatatcgac cttggtcgtc agcaaataaa catccgcgaa
    23341 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataatcgc
    23401 gggcagcgtg acgatacgaa accgttgcca ggcattagcg ccatccatct ctgccgcttc
    23461 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    23521 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcgaacca
    23581 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    23641 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    23701 gaggatcacc agcgaacgcg cggttttgcg cagacggaac tcgcggttga aaaacatcgc
    23761 cactgccagc ccgagcacgg tgctgcccgc caccaccagc gcggtatacc agaccgtcat
    23821 ccacagcgag tgccagaagc cgggatcgga gaggatacgc acatagttac tcaccccaac
    23881 aaaggttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    23941 caccattggc caggccacca gaccacccag cagtaataag ctgggcgcga gaagcagcag
    24001 cgcaaagggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    24061 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    24121 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacgcc agaccccgtc
    24181 acatcaccca tgcgggtaaa gtttttatcc cctactgcac caaagacctg aatatttggc
    24241 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    24301 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    24361 acccagtcgg caatgttgtc tgcctgctcc ataaaggtga caaatttttc tgctgcttcc
    24421 gtctcttcgg tcttttgccc ggcggtaatg gtcagcgagg tcaacatgcc gtagaccgca
    24481 gagtttttct cggttggcac cacgaaaccg acgtttttcg ggtcgccttc tttaatcaca
    24541 gccggaagga tataggtgga gtaaatcgcc atcggcgcgg tgccgttcat aaaggcgtct
    24601 ttcacttcca tgatgtcgtt agaacccggc atagtgttgg cagcaaggtc gcggtaatag
    24661 gtcagtgcct gcatcatctc tggtgtatca agggtgattt tgccttcggc gttaaagacg
    24721 ttagcctggt tggataacgc aaactgggag aaggattgtt ccgtcaacac gctttctgct
    24781 gtaggcagcg caatgccgta ttttttattc gccgggtcat tcagtttctg tgcaacgtcc
    24841 agcagctgtt gccagttttt cggctcctca agccccgctt ttgccagcac atctttgcga
    24901 taccagatac cgccaatcca ggcgctgaca ggaacaccgg tccatgcgct accatcttcg
    24961 gtacgcacaa tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt gctgatgact
    25021 gtggcaaccg ctttgcgatc gataagctgc tctttgtcca tcactttggc gtagtcatgg
    25081 ctggtttcta tcacttccgg cagcgaaccg ctacgtgaaa gagtaatgac tttagtgtta
    25141 taggcatctt cttccacggg cacttgctta acggtgatgc cagggttttc tttttcaaaa
    25201 cgggcaatca atttactgat aacggcctgg cgctcctgct ccaccgaaga atgcataaat
    25261 tcgatggata cattcgtttt attttcttct ttacaccctg aaatcagggc gcaggaaacc
    25321 agtgctgata acagcacgat ttttgattta atcatgagat gttccttttt atttaatcca
    25381 cataacctgc caggggcgca gcgttatttc attacccaat atatcaactt cactaatcag
    25441 atcccgacca tgcagattag taatatttac atgctgaata tttttactga cattaaacaa
    25501 tccagtcaga caattaccat cagcgttact tctttgaata cgcattacgg atgaattaat
    25561 cgtatcgatg gtaaaattat tatccggatg aaactcgtta tggctgcgac gaagtgtaat
    25621 taaacgcgac aactcatgat ataccgcatg ccttaatgta gcttcatcgt tcagttctcg
    25681 ggttatctct ttactgtgat attttttacg gttaatcgca cggttatatc cgagtttttc
    25741 gacacctgcg taatcattac gcgagccaag aatactttga atatatatcg ctggcacacc
    25801 ggggaaactt aacaaaatcg catgggcgag aataaacctg gcgcaacgtt cttcatcgct
    25861 actctcacgg cggcttaacg catccatata ggtcacattt atttcatacg gactgcgtgt
    25921 accgtcggga ttatttttcc agtttactaa tgcaccttcc tgctgtaacg cctcgaccag
    25981 ctctaatatt tcgctttcag gcaacaagcc ccgtagcggg tttagcccga tgccatcgtg
    26041 agaggcgagg aagttaaacc aggtggtgtt gctggaaggt agtgtcaggt tttgcgccca
    26101 cgcacaaagc gcctcaacgt tctgtttttg caccgcatgc agcaccagtg gcggcagcga
    26161 gaactggtac accatatgtg cttcgtcatc gcctgcgcca aagtaagcaa tgttgtcttt
    26221 atgcggaaca ttggtctcgg taatgatcac tgtacctggc gcaacgttat caataatcga
    26281 ccgtaacagt ttgataatca gatgtgtttt ttccagatgg atgcagcttg ttcccggctc
    26341 tttccacata aagccaacgg catccaggcg gacatactca gcacctttcg caaggtaaca
    26401 gagtaaaaca tccaccatcg ccagcaacac ttcagggcta cggtaattca ggtcaatttg
    26461 atcgtcacta aaggtggtcc ataaatggcg cgttgaatga tcgcgcatct ggaatggcgt
    26521 taataacggt aacgcacgcg ggcgagtgac ggcgctgaga tcggtttgcg ggtcaacggc
    26581 aataaaaaaa tcttcaaaac ctggatgctg ttgtaaatag tttttaaatc attcactttt
    26641 tgccgacata tggttgcaga caaaatcaaa cattaaatga ctgcattcac cgagttgctg
    26701 aatatcctgc cactcccccg cttcactggc gacctgatga taatcaatta ccgaaaagcc
    26761 atcatcagat gaccatggat aaaacggcaa caaatgaaca tgtgaaaaaa tgctttgcag
    26821 ccattgatgg taaaactgat taaatgtggg taatggtttt aaatcattgc tgtgaaattg
    26881 atcggcatag gtaattaaca cgacatcact ttcatcccag tgttttttac gtcgctgtgt
    26941 aattaatcgt ttcgcactct caagacgcgt tacaagtttc tgtaaatgag ttgcggtaaa
    27001 tgttccaccg tagatttcgt ccaggtaatc cgtaattttc tgtttcatgt tgcctttccg
    27061 tggtagcggt cccatgaaac gagagactac tccgcagaaa ataaactgtc aattgagcac
    27121 aagggtttta gtcacgcagg aaatcatttt ttaccaggga aaaagcgtat ttgtgagctg
    27181 acgcaaataa tctatgggga tgtaaataag gtgtgacaga aaaaaaacgg cgcataagcg
    27241 ccgctcatgg tgaattcttt taacctttga ccttcggcat tgcgatgtct ttcaggccac
    27301 cggcgttctc aacgtgggta tatcccatct cgctaaggat ctcttttgct tgccctgact
    27361 ggcgtccggc attgcaatac actttcacgg tgtcgttttt atccggaacg gcggtggcaa
    27421 tgcgctcttt cacttccttc aggggaatat tgatggcccc ctgaacgtgc tcttgctgat
    27481 actgctctgg aacacgaaca tcgatccagt gttcagcggc gaaaacgggc agtgaaaaca
    27541 ccagtgccag agcaagtaag ccttttttaa acatgtaagc gtccttctat ggatgaaaat
    27601 tgccatccat tatacgcctg agatttaaca atttttgcga aactcccctc accttttata
    27661 acgctgtgcc agtttattag cagcccgact taacagcggt tccagcgcca cagccagcaa
    27721 cattttcagc ggtcggcgag caactgattt tatcgcccag cccgctacac ccgccgggcc
    27781 atagcgcagt gcggtaagaa gtaccagctt gcctgctaat ttgaaaccag gctttacctt
    27841 ttgcccggcc tgttgccagc gagtattcat aactttcctc acagttgacg gaaacggcta
    27901 cgtaacgtga aagtatcgga agtgacataa cgttccatct cgcgtaaacg cgtttcactt
    27961 gccgccagtt cacggtcgac ttcatccagc aattcgctgc tggaaggtag ctgctcacca
    28021 aaggccatgt tgtccggcat tggatcaagc gcaaatgaca aaatgatgta agcaaccagg
    28081 gtaaacagcg ccagaccgaa gaaaatcgac agcaccacca ggatacgcac cagttttgcc
    28141 ggtacatcaa aatagttggc aatcccggcg cagacgccgc ggaccatgcc ctgctgtgga
    28201 atacgccata attttttatt gagattaatg cccgccatta gcgatccctc cagttcggat
    28261 gttctgcatc aagaatagat tccagcgcct gaatacgttc gcgcatccgt tttgcttcat
    28321 cagccagttg cgctaatcgc tgctgctcac tttgcgacaa ttcactgcga ccagaacgat
    28381 tgctgtaatg cagccataac cagatcggta aaacaaacag cacaaaaatg gttaacggaa
    28441 tagccagaaa tagcgcgctc ataagtactc cttagacgat gagcggcggc gcgttcggac
    28501 gccgcctgga tgttattatt gattgtcttg cttcattttg gcttttaatt gtgccagttg
    28561 ttcgctgatt gcatcatcgg ctttcagttc ggcaaactga tcgtccagcg atttttgttt
    28621 accgaagctg tggctttctg cttccgcttc catctggtca atacgacgtt cgaaagattc
    28681 gaaacgagcc attgcttcat ccagtttgcc actgtccagc tgacgacgca catcgcgcga
    28741 cgagtttgcc gcctgatgac gtaacatcaa tgcctgctgg cgagcgcgtg tttcgctcaa
    28801 tttgttttcc agctcaccga tctctttctt catgcgtgcc agcgtgtcgt ccaccagcgt
    28861 cacttcatgt tccaggctct taatcagatc ggtcagtttc tgtttttcaa ttaacgctgc
    28921 acgtgccaga tcctctctct ctttcagcag cgccagttcg gctttttcct gccattcaac
    28981 ttcacgcgcc gacgcttgtt caatacggcg agtcagctgt ttcttttctg ccaacgcgcg
    29041 cgccgaagta gaacgtactt caaccagtgt atcttccatc tcctggatca tcagacgaac
    29101 cagtttctgt ggatcttccg ctttctctaa cagagcgttg atgttggcat tcacgatgtc
    29161 ggcaaagcga gaaaaaatac ccataatgtt gtcctcttga tttctgcgtt caggattgtc
    29221 ctgctgaact gttaatacaa tttgcgtgcc aattttttat ctttttgatt tataaagatc
    29281 tgattgaaga accaacagca acatgccagg atgagttagc gaattacact aacaagtggc
    29341 gaatttcatc atggcagaat acaaagataa tttacttggt gaggcgaaca gctttctcga
    29401 agtgctggaa caggtttcgc atctcgcacc gctggacaaa ccggtgctca tcatcggcga
    29461 acgcggcacc ggtaaagagc tgattgccag ccgcctgcat tatctctcct cccgttggca
    29521 agggccgttt atttccctta actgcgcggc gttaaatgaa aatctgctgg attccgaact
    29581 gtttggtcac gaagcggggg cgtttaccgg tgcgcaaaaa cgtcatcctg ggagatttga
    29641 acgtgccgac ggcggtacgc tatttcttga tgaactcgct acggcaccga tgatggtgca
    29701 ggagaaatta ttgcgcgtga ttgagtacgg tgaactggag cgcgttggcg gcagccagcc
    29761 attgcaggtg aatgtgcggt tggtatgcgc gacgaatgcc gatctcccgg cgatggtcaa
    29821 tgaaggcact tttcgcgctg acctgctcga ccgactggct tttgatgttg tacaactgcc
    29881 accactgcgc gagcgcgaaa gcgacataat gttgatggca gaacactttg ccatccagat
    29941 gtgtcgggaa attaagctgc ctctgttccc ggggtttaca gagcgcgcca gagaaacatt
    30001 gctgaattat cgttggccgg gaaatattcg tgaattgaaa aacgtggtgg aacgttcagt
    30061 gtatcgccac ggcaccagcg attatccgct tgatgacatc attattgatc cctttaaacg
    30121 gcgtccgcct gaagacgcta tcgccgtttc agaaaccacc tcgcttccaa cactgccgct
    30181 ggatttacgt gagtttcaga tgcagcagga aaaagagttg ctgcaactca gtttgcaaca
    30241 agggaaatat aaccagaagc gtgcggctga attactgggg ttaacttatc atcagttccg
    30301 cgcgttgttg aaaaagcacc agatttagcg aagttgagca atacaacttg ccggatgcgg
    30361 cgtgaatgct ttattcggca tacataatca atacgtcgtt tgcaggcctg ataagcgcag
    30421 cgcatcagac attattgcgt tgggctatta atcgctcagc gcatcctgca aaattttcat
    30481 tgccgcatcg aattgcgcat ccggtatggt cagcggatac aggaagcgaa tcacgttgcc
    30541 gtatgcgcca caggtcagca ggagcagccc ctgcgccagc gcgcgttgct ggattttctg
    30601 tgcaatcgcc gctgacggct cgcccgtttg cggatcgtta aactctgccg caatcatcga
    30661 ccccaggccg cgtaccgcag caatggccgg aacgctttct ttggcatcaa tcaacgtgtt
    30721 tttgagacgc tggcccagtt gattcgcgcg ttcgcagagt gattctttgt cgataatatt
    30781 gagcaccgcg tgcgcggcag ccaccgccag cgggttcccg gcgtaggtgc cacccaaccc
    30841 gcccggcgcg ggcgcgtcca taatattcgc gttaccgacc acgcccgaaa gcggcatccc
    30901 gcccgcgagg cttttcgcca tcgtcattaa gtccggctta tcggcgtaat gatccatggc
    30961 aaacagttta ccggtacgcg caaagccgct ttgcacttca tcggcaatca tcacaatacc
    31021 gtgctcgtcg cacaggcggc gaatggcggc aaccagctct tttggcgcaa cgttgaaacc
    31081 gccctcgccc tgcaccggtt cgaaaataat ggccgcgacc tgcttcgctt cgatgtctga
    31141 tttaaacaag cgttcgatgg cgtcgaggga gtcctgtgtt gaaatgccgt gtaaatctga
    31201 cggataaggt acgtgataca ccgaaccagg gaacgggccg aagccgattt tgtacggcgc
    31261 aacttttccg gtcagcgcca tggtcatata tgtacgaccg tggaagccac cgctaaacgc
    31321 aatcacgcca ggtcgtccgg tatgggcgcg agcaattttc accgcgtttt ccaccgcttc
    31381 cgcaccggta gtgaagaatg cggttttagc ctgcccgctc actggggcaa gggcgttaat
    31441 tttctccgcc agcgtgacgt agctttcgta cggcacaatc tgatacgccg tgtgggtaaa
    31501 ctgttgcaat tgctgctcca ccgccgcgac cagatcaggg tggcgatgcc cggtattcag
    31561 caccgcaatg cctgcggcga aatcgatgta ttcgttgccc tctacgtcct tcagcgtagc
    31621 gttttctgcc gactgggcga agaagttaca catcacgcca accccgcgtg gggtagcaga
    31681 aagacgacgc tgatggaatt cattgttgct catgtttcac accttttgac tcttcactgt
    31741 taatcggaaa ttaaacgccc agacgatcgc gcaggctgta ataagccgcg cccatcgcgg
    31801 taaatggcac gcgcagcgtg cgcccgccgg ggaacgggta atgcggcaga ttggcgaagg
    31861 catcgaaacg ttcggcgtcg ccgcgcagca gttcggcaat cagacgcccg gcaagatggg
    31921 tacaggtgac gccgtggccg ctgtagccct gcatgtaata gatgttggta tcaaggcgac
    31981 cgaactgcgg catgcgcgac aaggtcagca ggaagttgcc cgtccagcgg taatcaattt
    32041 tcacgccctt cagctgcggg aaggttttca gcagtttcgg caccacaagg cgctcaacgt
    32101 catccgggtc gcgcgcgccg tagaccacgc cgccgccgta cagcaggcgg ttgtcggcgg
    32161 taagacggta gtaatcaagc agatagttac agtcttccac acagtagttt ttcgggatca
    32221 gcgaacgggc taaatcttcc gacagccgtt cggtggtgat cacctgcgtg ccgcacggca
    32281 tgctgcgttt cgccagttcc ggctctactt tatcgcccag atacgcattc ccggcgacaa
    32341 tcacatactt cgccgtcacc tgacctttgg cagttcgcac aacggctggc gtggtgtgct
    32401 ggatctgcgt cacggcagaa agttcataca cgcgcccgcc gttgaggcgg atggcgtccg
    32461 cttcgccaat ggcgaggttt agcggatgaa tatgcccacc gctgtgatcc agcagcgcac
    32521 cggtatagcg atcgctggct acttcacggc gaatggcgtt ggcgtccagc aattccagct
    32581 gtttattgcc gtagcgttcc cagttctctt tctgctcttc aagtgtggcg agctgtttat
    32641 cattcatcgc cacaaacagg ccgccggggc ggtagtcgca gtcaatctga taacgtttga
    32701 tacgttcgcg gatgatctca ccaccttcga acatcatgct gccgagcata cgggcggtgt
    32761 ccatgccgta gcttttttcg atcacgtcga tgtcgcggct gtaggagttc acaagctgcc
    32821 cgccattgcg cccgcttgcg ccaaagccga tgcgtgaggc ttcgagaacc actacgtcaa
    32881 agcccgcttc cgccagatgc agcgcggaag agagcccggt atagccgccg ccgaccacgc
    32941 aaacgtcgca ggaaatcgac tcattcagcg tgtcgaatgg cgcgtattta ttcgcactgg
    33001 cggcgtagta actgctggta tgttcggtca tttcaggcct ccaggcttat ccagatggtt
    33061 ttcagttcgg tgaatttttc aagggcatgc agggatttgt cgcgaccgtt gccgctctgc
    33121 ttatagccgc caaacggcac ggtcatatcg ccgtcgttgt agttattgac gaagacggaa
    33181 ccagccttca ggcggcggct catgcggtgc gcgcgggaga ggtcgcgcgt ccataccgcc
    33241 gcgccaaggc cgtactggct gtcgttggca agctgtagcg cctggtcttc tgatgtgaaa
    33301 cgcgtgacca ccagcaccgg accgaaaatc tcttcgcggc ttaaggacgc attcgggtct
    33361 acatccacaa agatggtcgg gccgatggcg gcagccagct cagcgttacg accatccagc
    33421 agcagttgcc ctttgctttc gccttcccga ataaagctat ggaccgagtc agcgtgagcg
    33481 ctgtcgatta aggtgcccat ggtggttgcg ggatcaagtg gatggcccgg ctgccagttt
    33541 tgcgcctgct gttttaacag ggctaagaat tcatcggcga tgctctcttc cagtaacaaa
    33601 cgcgttccgg cgatgcacac ctggccctgg ttgtagaaga tgcctgcggc ggtggcgctt
    33661 gccgcttttt gcagatccgg acaatcggcg aaaacgatgt tggcgctttt gccgcccgct
    33721 tccagccaga cgcgtttcat gttgctctcg cccgcatctt tcagcagctg tttcccggta
    33781 cgggtcgaac cggtaaaggc aatggcgtcg atatcgttat gacgcgacag cgcctgcccc
    33841 gcttcatgac caaaacctgt caccacgttc aaaacaccat ccggcaagcc tgcttctttc
    33901 gccagccccg cgagacgaat cgcactgagc ggtgattttt cagacggttt caggacaacg
    33961 ctgttccctg ctgccagcgc cgggccaagc ttccagcagg tcagcagcag cggaaagttc
    34021 cacggtacga tggcggcaat cacaccgacc ggttcacgca cgatcatcgc cagctcatgg
    34081 ctactggtgg tcgccacttc gccatacact ttgtcgatcg cttcggcgta ccagcgaatg
    34141 gcgcgcgccg cgccgggaat atcatcacgc agactgtgac gaatcggttt gccggtgtcg
    34201 agagtttcca gcagtgccag ctcttcggcg tgggcttcca ttaaatcggt gagtttattc
    34261 agtaccgctt tacgtttagc cggagaagag agtgaccagt cgccgcgttc aaatacgccg
    34321 cgtgctgcgc tcaccgcacg gtcgatatcg acgctcttgc cgcgggcaat tttcgccagc
    34381 ggtgcctggg tgaccggatc aacggtttca aaggtttcat tttccgccgc agcagtatat
    34441 tcaccgttaa taaataagcg ggtttcaatg gcgagactta acgctttatc ctgccagtaa
    34501 gccagatgat gaaaattcat tatgactcct gtttcacgtc tatcagatat atgcagtttg
    34561 tgaacgtcag ggcgcagaga tccccgggcc gtatgcccgt ttatttctgg cgacattctt
    34621 tatatacact tcatccttca agccgcctct gccttggctg cgttcgctcc cccgaatcac
    34681 ttacctgagt aaactcatcg ggattcgctc acttgccgcc ttgatgcaac ttgaatgatt
    34741 ttgtgtatag attgccgggc gcattgcgct tacccggctt acagaacaaa aagattaaaa
    34801 cgtggtgggc gtatgggcgc tgataattcg gcaaatacct gccgacgtat tactgaaact
    34861 gtgcgggatg ccggtattaa tggcataact ttgccccgcg acgaggtggt aatcctgacc
    34921 attaatcgtc agaacaattt caccttccag tactgtgcct atttcctcac cctgatgctt
    34981 aattctttcc ccagtggttg tgcccggctg gtacgtttca aagatcatcg ccagcgtgcg
    35041 attcgggtta ccgttatgaa ccagcttcat tgacacaccc tgactgccca tctcaattaa
    35101 gtcgtcctga ttaatgacga cctgcggctc atcaggtttt tccggctcgg aaaagaattc
    35161 cgagagtgac agaccataca ccttcagcag cttttgcagc gtactgatgg cagggctgac
    35221 tttatcttgt tctatcgtac tgatagcact gtgagtcagc ccggagagtt cggcggcacg
    35281 acgttgtgaa agcccctgct gctggcggat ttccgacaag cgttttcctg gcgccagtcc
    35341 ctcatcactc atatttgcat ttccttaaac tgtagtggtc agagtcgctg tttttcagcg
    35401 atatggtgct gacaagcggt gataaagccc tcgaacaata tacgcgaaag cgcgtactcg
    35461 ctactgttcc attccgggtg ccactgtacg cccagcgcaa aaggatgatt gatgacgcta
    35521 accgcctcaa ctaagccatc cggcgagcga gcttcaacac gcaaccgtgg gctaacgacc
    35581 ttcgcccctt gcccatgcag agagtttacc caaaagttgc tacattcagg taacaacgca
    35641 gacagtaatc ccccctcttc aacctgaact tcatgcgacg gtgcatattg ctgttccacc
    35701 ggcagttcgg gatcttcccg atgttccagc aattcaggct gttcgcacag cttgcgatgc
    35761 aacgaaccac cggttgccac caccagttcc tgtaaacccc ggcagatggc gaaaatgggg
    35821 atgcgccttt cgagtgcggc attaattaac gccatgctca gaagatcacg cccgggatcg
    35881 gcgtcaggct catcgccgtt ttcaccatat aggtgcggct gcacattgct gggactacca
    35941 ggaagataaa tgccatcgag tttcggcaaa agttgttcaa gtaatgacgg ttccgccagc
    36001 gcatgtggta gcgcaattgg caagccgcct gcgtggatga tggcattcag gtacttttct
    36061 tgcagagtct gggtcgcatg acccttaagc ctgttcctgc acattacgac accgataacc
    36121 ggattgttca ttatattttc catgctcaat ctcacaaagt ggactaaatt atcgccatta
    36181 ctgcggttta aatgaccttt atgttcaata ttttttcaat ctagcagtgg gttttcattt
    36241 ttgcaaactc aatttaacat ttgacaaaca tttagtttgc atacagattc gaatggtggt
    36301 cattatattt tacgctttga taacgagcgg aaaacaaacc aaaggcgaag aatcatggaa
    36361 accaatatcg ttgaagtaga gaactttgtt cagcagtcag aagagaggcg gggtagcgcc
    36421 tttacgcagg aagtgaaacg ctacctggag cgctacccga atacgcaata tgttgatgtt
    36481 ttacttactg atttgaatgg ttgcttccgt ggaaaacgta tccctgtttc aagcctgaag
    36541 aagttggaga aagggtgtta tttcccggcc tcggtgtttg caatggatat tctgggcaac
    36601 gtagtagaag aagcgggtct gggtcaggaa atgggcgagc cggatcgtac ttgtgttcct
    36661 gttctcggtt ccttaacccc ttctgccgcc gatccagagt ttatcggtca gatgctcctg
    36721 accatggtcg atgaagatgg cgctcccttt gacgttgagc cgcggaacgt tcttaaccgc
    36781 ctgtggcagc agctacgcca gcgcggattg ttcccggttg tagcggtaga gctggagttc
    36841 tatttactgg atcgtcagcg cgacgctgaa gggtatctgc aaccgccctg cgcgcccggc
    36901 accgatgacc gcaatacaca aagccaggtt tactccgttg ataacctcaa ccacttcgcc
    36961 gatgtgctca atgatattga tgaactggcg cagttacagt taattcccgc cgacggtgcg
    37021 gtcgccgaag cctcgccggg tcagtttgaa atcaaccttt accataccga taacgtgctg
    37081 gaagcctgcg atgatgcgct ggcgctaaaa cgcctggtgc gtctgatggc agaaaagcat
    37141 aagatgcacg ccacttttat ggcgaagccg tatgaagagc acgcgggcag cggaatgcat
    37201 atccatatca gtatgcaaaa taaccgtggc gagaacgtgc tttctgacgc ggaaggcgaa
    37261 gattcgccgc tgctgaaaaa gatgctcgcc gggatgattg acctgatgcc gtcgtcgatg
    37321 gcgttgctgg caccaaacgt gaactcgtat cgccgcttcc agccgggaat gtatgtgccg
    37381 acgcaggcgt cgtggggcca taacaaccgc accgtcgccc tgcgtattcc gtgcggcgac
    37441 cgtcataatc accgcgtgga atatcgcgtg gcgggtgccg atgccaaccc atatctggtg
    37501 atggcagcga tttttgccgg tattttgcat ggccttgata acgagctgcc gttgcaggaa
    37561 gaagtcgaag gcaacgggct ggaacaggaa ggcttaccct tcccgattcg ccagagcgat
    37621 gccctgggtg agtttatcga gaatgatcac ctgcgccgct atttaggcga acggttttgc
    37681 catgtgtatc acgcctgtaa aaatgatgag ctgttgcagt ttgagcgcct catcacagaa
    37741 acagaaattg agtggatgtt gaaaaacgcg taattcccca tgcggggttg tttcatcggc
    37801 ctgagcggag ccaggcctga catttgctgg taatccagac atttcccgga tgtcgcgtta
    37861 tggagcgcag gcggcaacgg gcttccgtta acgattaaga ctgtgacgag gaaatgagat
    37921 gatgccgatg attaaatccc cgcatggcga gggaggctgc gtatgcgccc ctcctgctac
    37981 tgactggact ccgccccctt tgcttccctt gctaaacagg tttgatttca ggtcgacacg
    38041 accgcaaacc ttattacgca ggggaggcag caattatggc tattaattca ccactgaata
    38101 ttgctgcgca acccggcaaa acccgtctgc gaaaatcact gaaattgtgg caggtggtaa
    38161 tgatgggtct ggcctatctc acgccgatga ccgtatttga tacttttggt attgtctccg
    38221 gcattagcga cggtcacgtt ccagcgtcct atttgctggc gctggcgggc gtgctgttta
    38281 ccgctatcag ctacggcaaa ctggttcgcc agtttccgga ggccggttcg gcctatacct
    38341 acgcgcaaaa gtcgattaat ccgcacgtcg gatttatggt cggctggtca tcactgctgg
    38401 attatctctt tttgccaatg atcaacgttt tgttggcgaa aatctatctc tccgccctct
    38461 tcccggaagt gccgccgtgg gtgtgggtgg taaccttcgt cgccatttta accgccgcga
    38521 atctgaagag cgtcaacctg gtcgctaact tcaataccct gtttgtactg gtgcaaatct
    38581 ccatcatggt ggtgtttatc ttcctggtgg ttcagggact gcataaagga gaaggcgttg
    38641 gcaccgtctg gtcacttcag ccgtttatca gcgagaacgc gcacctgatc ccgattatta
    38701 ccggtgcgac gattgtctgt ttctcgttcc tcggtttcga tgcggtgacc acgctttcgg
    38761 aagagacgcc ggacgccgca cgcgtgatcc cgaaagccat cttcctgacg gcggtatacg
    38821 gcggcgttat ctttatcgcg gcgtcgttct ttatgcagct gttctttccc gatatcagcc
    38881 gctttaaaga cccggacgcc gcactgcctg aaattgcgct gttcgtcggc ggcaagctct
    38941 ttcagtcgat tttcctctgc accacgtttg tgaacacatt agcgtctggc ctggcttcac
    39001 atgccagcgt gtcgcgtctg ctgtatgtga tggggcgtga caatgtgttt ccggagcgcg
    39061 tgtttggcta tgtgcaccca aaatggcgga ctccggcact caacgtcatt atggtcggga
    39121 ttgtcgcgct gtcggcgctg ttctttgatt tagtcaccgc gacagcactg attaacttcg
    39181 gtgctctggt ggcgtttacc ttcgtgaatc tgtcggtgtt taatcatttc tggcggcgta
    39241 aaggaatgaa taaaagctgg aaggatcact tccactattt gctgatgccg ctggttggcg
    39301 cgctgacggt gggtgtgctg tgggttaacc tagagtcaac gtcactgaca ctcggtctgg
    39361 tatgggcttc gttaggcggc gcatatttgt ggtatctgat ccgccgctat cgcaaagtgc
    39421 cgctgtacga aggtgacaga acgccggtga gcgaaacgta atagccggat gcgcggcgca
    39481 ttatcctgcc tgagggtaac gcgccgcgcc attaactata aaaagatgta tgacccggat
    39541 cacgagtgct atcatccgcc cttatgctta gcgcattaac cccaggggca aataatgaac
    39601 cacgatatcc cgctaaaata ttttgatatt gccgatgagt acgcgaccga atgcgcagag
    39661 cctgtcgcag aggctgaacg cacaccgctg gcccactact tccagttact gctcacccgt
    39721 ttgatgaaca atgaagagat cagcgaagaa gcccaacatg aaatggctgc tgaagcgggg
    39781 attaatcccg tacgcattga tgagatcgca gagtttttga atcaatgggg taatgagtaa
    39841 ttatttaatt gcagacgatc ggtcagtatc gttgtccgga taaagcgttc acgccgcatc
    39901 cggctaacaa tgacacgcac tgcagcgtgc tttaatgggt ttctcacgat cgtaccgctc
    39961 tgctcctcgc taagttgttc tgtgagttat agttatagag aaatcacggg tacgaatgtt
    40021 gaacagatgg cgacgccacc tgttcaacga ccaaccacac ggccctcatt acgacccctg
    40081 aacacaaacg gtaatggcaa atgtcattaa atattagtaa gatagcctct ttacataaaa
    40141 tcccttaata ttatgcgcca ggtattatcg tctcttttgg tgattgctgg acttgtgagt
    40201 ggtcaggcaa tcgccgcgcc tgaatccccc ccgcatgctg atatccgcga cagcggtttt
    40261 gtctattgcg tcagcgggca agtcaacacc tttaacccat ccaaagcgag cagtgggtta
    40321 attgtcgata cccttgccgc ccagttttat gatcgactgc tggatgtcga tccctatacc
    40381 tatcgcctga tgccggaact tgccgaaagc tgggaagtac tcgacaacgg cgcgacctat
    40441 cgcttccacc tgcgtcgcga tgttccgttt caaaaaaccg actggtttac tcccactcgt
    40501 aaaatgaatg ccgacgatgt ggtgtttacc ttccagcgaa tttttgaccg caacaacccg
    40561 tggcataacg tcaacggcag caacttcccc tacttcgaca gcctgcaatt tgccgataac
    40621 gtgaaaagcg tccgcaaact ggataatcat accgttgagt tccgactcgc ccagccggat
    40681 gcttcttttt tgtggcacct cgcaacccat tatgcttcgg tcatgtcggc agaatatgcc
    40741 cggaagttag agaaagaaga tcgccaggag caactcgacc gtcaaccggt cggcaccggt
    40801 ccgtatcagt tgtcggaata ccgcgccggg caatttattc gcctacaacg tcatgatgac
    40861 ttctggcgcg gtaaaccgtt aatgccgcag gtagtggtgg atttaggctc cggcggcacc
    40921 ggacgtctgt cgaaactcct gaccggggaa tgcgacgttc tggcctggcc tgctgccagc
    40981 cagctatcca ttttgcgtga cgacccgcgc ttgcgtttaa cgctgcgtcc tgggatgaac
    41041 gtcgcctatc tggcatttaa caccgccaaa ccgccgctaa ataatcccgc tgtccgccat
    41101 gcactggcac tggcgattaa taaccagcgc ctgatgcaat ccatctatta tggtacggct
    41161 gaaacggcgg cctctatttt accgcgcgcc tcgtgggcct atgacaacga ggctaaaatt
    41221 actgaataca atccggcgaa atcgcgcgaa cagttgaagg cgttggggct ggaaaactta
    41281 acgctgaaac tgtgggtgcc cacacgttcg caggcgtgga accccagtcc actgaaaact
    41341 gccgaactga ttcaggcgga tatggcgcag gttggcgtaa aagtggtgat tgtgccggta
    41401 gaaggtcgct ttcaggaggc gcggttgatg gatatgagcc atgatctgac gttatccggt
    41461 tgggcgacgg acagtaacga cccggacagt ttcttccgtc ctttactgag ctgcgcggca
    41521 attcattcac agaccaacct cgcccactgg tgcgatccga aattcgacag cgtgttgcgt
    41581 aaggcgctct cctcgcagca gctggcggcg cgtattgaag cctatgacga agcgcagagt
    41641 attctggcgc aggaattgcc cattttgccg ctggcgtcgt cattgcgttt gcaggcctat
    41701 cggtacgata tcaaaggtct ggtacttagc ccgtttggta acgcctcctt tgctggggtg
    41761 tatcgcgaga aacaggatga ggtgaaaaaa ccatgattat cttcacctta cgccgcattt
    41821 tgttattgat tgtcaccttg ttcctgctga ccttcgttgg cttcagcttg agctatttca
    41881 cccctcatgc gccgttacaa ggcgcgtcac tgtggaatgc ctgggtgttc tggtttaacg
    41941 gcctgatcca ctgggatttt ggtgtttcca gcattaacgg tcaaccgatt gccgaacagt
    42001 taaaagaggt cttcccggcg acgatggaac tgtgcatcct cgccttcggt tttgcgctga
    42061 ttgttggcat ccccgtgggg atgattgccg ggattacgcg ccataagtgg caggataact
    42121 tgatcaacgc catcgcctta ctgggctttt caatcccggt gttctggctg gcgcttctgt
    42181 tgacgctgtt ttgttcactc acgctaggtt ggttgcccgt ttcagggcgt ttcgatctgc
    42241 tctacgaagt gaaaccgatt accggttttg cgttgattga tgcctggctt tcggactcac
    42301 catggcggga tgaaatgatc atgagcgcaa tccgccatat gatattgccc gtgatcaccc
    42361 tgtcggttgc gccaacaact gaagtgatcc gcctgatgcg tatcagcact atcgaagtgt
    42421 atgaccagaa ctatgtaaaa gcggcggcga cccgcggttt gtcgcgcttt actattttgc
    42481 gtcgccacgt tctgcataac gcgttaccgc cggttattcc tcgcctgggc ttacagtttt
    42541 ccaccatgct gacgctggcg atgatcaccg aaatggtttt tagctggccg ggtttagggc
    42601 gctggttaat taacgccatc cgccagcagg attacgcagc catttccgcc ggagtgatgg
    42661 tgtgtggctc actggtcatt attgttaacg tgatttctga tattttgggt gccatggcta
    42721 accctctgaa acataaggaa tggtatgcct tacgatagcg tatacagcga aaagcgcccg
    42781 ccgggcacgc tgcgtaccgc ctggcgcaaa ttttatagtg acgcctctgc gatggtcggc
    42841 ctgtacggct gcgcggggct ggctgtactg tgtatttttg gcggctggtt tgcgccttac
    42901 ggcatcgacc agcaatttct cggttatcaa ttattgccgc cgtcatggtc gcgctatggc
    42961 gaagtttctt tcttcctggg gactgacgac ctcggacgcg atgttttaag ccgtttactg
    43021 agcggagctg cgccaaccgt gggtggcgca tttgtggtga cgcttgccgc gacgatctgt
    43081 ggcctggtac tgggcacctt cgccggggcg acacacggcc tccgctcagc ggtgcttaac
    43141 catattctgg ataccttact ggcgatccct tcgctgctgt tggcaattat cgttgtagcg
    43201 tttgccggac cgagtttgtc ccacgctatg tttgctgtct ggctggcatt gctgccgcgt
    43261 atggtacgtt cgatttacag catggtgcat gacgaactgg aaaaagagta cgttatcgcc
    43321 gcccgtctgg atggcgcatc aacgctgaat attctctggt ttgctgtgat gccaaacatc
    43381 accgccgggc tggtcaccga gatcacccgc gcactgtcga tggcaattct cgatatcgcc
    43441 gcgctgggct ttctcgatct cggcgcacaa ctcccctcgc ctgaatgggg agcgatgctc
    43501 ggtgatgcgc tggaactgat ttatgtcgcc ccgtggactg tcatgctgcc aggtgcggca
    43561 attatgatta gcgtgttgtt ggttaacctc cttggcgatg gcgttcgtcg tgcgattatt
    43621 gcgggggtgg aataatgcca ttactcgata ttcgtaacct gaccattgaa tttaaaaccg
    43681 gtgatgagtg ggttaaagcc gtcgaccggg taagtatgac cttaaccgaa ggtgaaatcc
    43741 gcggtcttgt tggtgaatcc ggttccggca agagtttgat tgcgaaagcg atttgtgggg
    43801 tgaataaaga taactggcgt gttactgctg accgtatgcg ttttgatgat atcgatttgc
    43861 tgcgtctctc cgcacgcgaa cggcgcaaac tggttggcca taacgtgtcg atgattttcc
    43921 aggaaccgca gtcgtgtctt gacccttcag aacgtgtggg tcgccagttg atgcaaaaca
    43981 tcccagcctg gacctataaa ggccgttggt ggcagcgttt tggctggcgc aaacgccgtg
    44041 ccattgaact gctgcaccgc gtggggatta aagatcacaa agatgcgatg cgcagttttc
    44101 cctatgagtt gaccgaaggt gaatgtcaga aagtgatgat agccattgca ctggcgaatc
    44161 aaccgcgtct gctgattgct gacgaaccga ccaactcaat ggagccaaca acccaggcgc
    44221 aaatctttcg cctgctgacg cgtctcaacc aaaacagcaa taccactatt ttgcttatca
    44281 gccatgactt acaaatgctt agccaatggg cggataaaat taacgtgctt tactgcggtc
    44341 aaacagtgga aaccgcgccg agtaaggagt tggtgacgat gccacatcat ccttataccc
    44401 aggcgctgat ccgcgcgata ccagacttcg gcagcgcgat gccgcataaa agtcgcctca
    44461 atacgctgcc cggcgctatc ccactgctgg aacagttacc gattggttgt cgtctggggc
    44521 cacgttgccc gtatgcacaa cgagaatgca ttgtgacgcc acgtttgacg ggggcgaaaa
    44581 atcatctcta tgcctgtcat ttcccgctga acatggagaa agagtgagat gatcgaaacg
    44641 ctgcttgaag tgcgtaattt aagtaaaacg ttccgctacc ggaccggctg gtttcgtcgt
    44701 cagaccgtag aagcggtaaa acccttgagc tttacgctac gtgaaggcca gacactggcg
    44761 attattggcg agaatggttc gggtaaatcc acgctggcaa aaatgctggc gggaatgata
    44821 gagcctacca gcggcgagtt attgattgac gatcatccac tgcattttgg cgattattcc
    44881 ttccgcagtc agcgtattcg catgattttt caggatcctt cgacctcgtt gaatccccgt
    44941 caacgtatct cgcaaattct ggattttcca ctgcgcctga acaccgacct ggaaccggaa
    45001 cagcgtcgca aacagattat tgaaacgatg cgtatggttg ggctgctacc ggatcacgtc
    45061 agttactatc cgcatatgtt ggcacccgga caaaaacagc gtctgggtct ggcgcgcgcg
    45121 ctgatattgc gcccaaaagt cattattgcc gatgaagccc tcgcttcact ggatatgtcg
    45181 atgcgttcgc agttgattaa tctgatgctg gagttacagg aaaaacaggg catttcgtat
    45241 atttatgtta cccagcatat cggaatgatg aagcacatta gcgaccaggt gctggtgatg
    45301 catcagggcg aggttgtcga acgaggcagc accgcggatg tgctggcatc gccgctgcat
    45361 gaactcacca aacggctgat tgccgggcac tttggcgaag cattgacagc ggatgcgtgg
    45421 cgtaaagatc gctaattttc tgaatcctcc ttccagatta tccacctcat tctgacaaga
    45481 gattaaatct tgtcagaatg aggtgatgga taaaataaaa tcaaatgcgc gtgatttaag
    45541 acgcaatctc actttgcagg aacgaaagct ctggcgatat cttcgcagcc gacgttttgg
    45601 tgatttcaaa tttcgccgtc aacatccggt ggggagctac attctcgatt ttgcttgctg
    45661 ctcggcgcgc gtagtcgttg agctggatgg tgggcagcat gatttggcag ttgcctatga
    45721 taccaggcgc actagctggc ttgagtcgca gagctggacc gtgctgcgtt tctggcataa
    45781 cgagattgat tgtaatgagg aggcggtgct ggagattatt ctgcaggaac tgaaccgccg
    45841 gtcaccctca ccctgaaagg gcgagggggc cgaccgagcc gaatagctgt tgtggtgaaa
    45901 acatggagac agtgctggag aatattcggc aaggtctgaa ccatcccagc catcgccata
    45961 aaagggttag gggctgtatg agcctgtttg ttgctggggt aacaatattt gcacaatatg
    46021 gtcccctcgc ccctctgggg agagggttag ggtaagggga aaagcgccca ccctgccgca
    46081 gcctgctccg gtcggacctg gcaactatag ctactcacag ccaggttgat tataataacc
    46141 gtttatctgt tcgtactgtt tactaaaacg acgaatcgcc tgattttcag gcacaacaag
    46201 catcaacaat aaggattaaa gctatgggtt ttctttccgg taagcgcatt ctggtaaccg
    46261 gtgttgccag caaactatcc atcgcctacg gtatcgctca ggcgatgcac cgcgaaggag
    46321 ctgaactggc attcacctac cagaacgaca aactgaaagg ccgcgtagaa gaatttgccg
    46381 ctcaattggg ttctgacatc gttctgcagt gcgatgttgc agaagatgcc agcatcgaca
    46441 ccatgttcgc tgaactgggg aaagtttggc cgaaatttga cggtttcgta cactctattg
    46501 gttttgcacc tggcgatcag ctggatggtg actatgttaa cgccgttacc cgtgaaggct
    46561 tcaaaattgc ccacgacatc agctcctaca gcttcgttgc aatggcaaaa gcttgccgct
    46621 ccatgctgaa tccgggttct gccctgctga ccctttccta ccttggcgct gagcgcgcta
    46681 tcccgaacta caacgttatg ggtctggcaa aagcgtctct ggaagcgaac gtgcgctata
    46741 tggcgaacgc gatgggtccg gaaggtgtgc gtgttaacgc catctctgct ggtccgatcc
    46801 gtactctggc ggcctccggt atcaaagact tccgcaaaat gctggctcat tgcgaagccg
    46861 ttaccccgat tcgccgtacc gttactattg aagatgtggg taactccgcg gcattcctgt
    46921 gctccgatct ctctgccggt atctccggtg aagtggtcca cgttgacggc ggtttcagca
    46981 tcgctgcaat gaacgaactc gaattgaaat aatcattctg ttggtaaata ttgggcggcg
    47041 ttctgccgcc cgttatctct gttatacctt tctgatattt gttatcgccg atccgtcttt
    47101 ctccccttcc cgccttgcgt caggataacg atttccttta cgaccaagga gcgcccatgg
    47161 aacaacgcca catcaccggc aaaagccact ggtatcatga aacgcaatcc agtactgcgg
    47221 agtatgacgt tctgcctctg gtcccggaag ccgcaaaggt cagcgatccc tttctgctcg
    47281 acgtgatcct tgatgaagaa acgctggctc ccttcctttc atggctggtc cctgcgcgcg
    47341 ttcttgcagt ggaattgttc cctgaccagc ttaccgtgac ccgttcacag accttcaccg
    47401 cttatgaacg cttgtctacg gccctgacgg ttgctcaggt ttgcggcgtc cagcggttat
    47461 gtaactacta ttcggcgcga cttacgccgc tccccgggcc tgattcctcc agggaaagca
    47521 atcataggtt ggcacaaatc acgcaatatg cccgccaact ggctagctcg ccttctatta
    47581 tcgacaaccg atcgcgccag catctgaatg acgtcggtct tactgccggg gactgtgtga
    47641 tcattaacca aatcattggt tttattggct ttcaggcgcg gaccattgcg acatttcagg
    47701 cttatctcgg gcacccggta cgctggttac ccggtctgga aatacaaaac tacgccgacg
    47761 cgtcactgtt tgctgatgaa tcaatacgct ggcgaagcag ctatgaggtg gaaaaactac
    47821 ctgaagagta cacaaaaagt tcaacagcag aactttgcca actggccgaa acactctctc
    47881 tccaccctat ttcactttcc cttctcgaaa ggttgttaaa cagtacacgg gttaatacac
    47941 agccggataa taagcttgcg gcgttgttat gcgcgcggat aaatggcagt cctgcttgtt
    48001 ttgccgcctg tatggattca tcaaatgaat ataaaaaaat cagccccctt ctgcgcaagg
    48061 gcgaaaatga aattaaccaa tgggctgacc gtcattctgt tgagcacgct accgttcagg
    48121 cgatacaatg gctgacccga gcacccgatc gctttagcgc cgcccagttc agccctttac
    48181 tcgaacacga aaaatcatca acgcagatta ttaatctgct ggtatggagc gggctgtgtg
    48241 gctggataaa tcgcttaaaa atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac
    48301 agattcgcgt aaaactgtca gccgctctaa tggccaccaa aatagacaat tatgtttcag
    48361 gacaacccgc tgctagcgca gcttaaacag caactgcatt cccagacgcc acgcgctgaa
    48421 ggggtggtaa aagccacaga aaaaggcttt ggcttcctgg aagtcgacgc gcaaaaaagt
    48481 tatttcattc cgccgccgca gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg
    48541 atccacagtg aaaaagaacg tgaatccgca gagccagaag aactggttga accgttcctg
    48601 actcgtttcg tgggtaaagt tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat
    48661 ccactcttaa aagacgccat tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa
    48721 gaaggcgact gggcggttgc cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc
    48781 tatgctgaac tgacacaata catcactttt ggtgacgacc actttgtacc gtggtgggtt
    48841 acccttgcgc gccataatct ggaaaaagaa gcaccagacg gcgtcgctac cgcaatgctc
    48901 gatgaaggtc tggttcgtga agatctgacc gcgctggatt ttgtcaccat cgacagtgcc
    48961 agcacagaag atatggatga cgcccttttc gctaaggcgt tgccggatga caaacttcag
    49021 ctgattgtgg cgattgccga tccaaccgcg tggattgctg aaggcagcaa gctggacaaa
    49081 gccgcgaaaa ttcgcgcatt caccaactat ctgcctggct tcaacatccc tatgctgcct
    49141 cgcgagcttt ctgacgatct ctgctcactg cgcgccaatg aagtccgccc ggtactggca
    49201 tgccgcatga cgctctccgc tgatggcacc attgaagata atatcgaatt ctttgccgcc
    49261 accatcgaat ccaaagcaaa gctggtgtat gaccaggttt ctgactggct ggaaaatacc
    49321 ggtgactggc agcctgaaag tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt
    49381 tgccaacgcc gcggcgagtg gcgtcataac cacgcactgg tgtttaaaga tcgcccggat
    49441 taccgcttta ttctcggtga aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc
    49501 attgccaacc gtatcgtcga agaagcgatg attgccgcta acatttgtgc ggcccgcgta
    49561 ctgcgcgata agctcggttt tggcatctat aacgtgcata tgggctttga tccggcgaat
    49621 gccgacgcgc tggcagcgtt gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg
    49681 ctcacgctgg acggtttctg caaactgcgt cgtgaactgg acgcgcaacc aactggtttc
    49741 ctcgacagcc gcattcgtcg cttccagtca tttgctgaaa ttagcactga acccggtcct
    49801 cactttggcc tcggtctgga agcatacgcc acctggactt cgccgatccg taaatatggc
    49861 gacatgatca accaccgtct gctgaaagcg gttatcaaag gcgaaactgc gacgcgccca
    49921 caggatgaaa tcactgtcca aatggccgag cgtcgccgtc tcaaccggat ggcagaacgt
    49981 gatgttggtg actggttata cgcacgcttc ctgaaagaca aagccgggac cgacacccgt
    50041 ttcgcagcgg aaattgtcga tatcagccgt ggcggcatgc gtgttcgttt ggttgataac
    50101 ggcgctatcg cctttattcc ggcacctttc ttacacgcta tgcgcgatga actggtttgc
    50161 agccaggaaa acggcactgt acaaattaaa ggtgaaacgg tttacaaagt gactgacgtt
    50221 attgacgtca ctattgccga agtccgcatg gaaacccgca gcattattgc gcgcccggtc
    50281 gcgtaatctc ctttcacggc ccattcctca tggatgggcc gtttatttcc ccgctctacc
    50341 ttcgttatat ccaagcagat tttttaactg actttcgttt gaaaactggc gtttttccaa
    50401 aactggatta caaataaata tataaaaaaa acaatgcgtt tagccgccgt taatttcatg
    50461 taagtcatgt tgttcctttt atttaaccgc ggactccgca gttaaccgga ggatatgcat
    50521 catgaaaacc gttagggagt ccacaacgtt gtacaacttt ctcggatcgc acaatccata
    50581 ctggcggttg acggaaagca gcgatgtttt gcgcttttct accaccgaaa ccacagaacc
    50641 tgatcgtaca ttgcagttat ctgccgaaca ggctgctcgc atcagggaaa tgacggtcat
    50701 cacctccagc ctgatgatga gtctgaccgt cgatgaaagc gatctttctg tgcatctggt
    50761 aggacgaaaa atcaataaac gggaatgggc tggcaacgcg tctgcatggc atgacacacc
    50821 ggcggttgct cgtgatttat cacacgggct ttcctttgct gagcaggtag tttctgaagc
    50881 acattccgca atagtgattc tcgacagccg ggggaatatc caacgcttca atcggttatg
    50941 tgaagattac acagggttga aagaacacga cgtcattggg caaagcgtgt ttaaactgtt
    51001 tatgagccgt cgtgaagctg cggcatccag gcgcaataac cgtgtatttt ttcgaagcgg
    51061 caatgcatat gaagtcgaac tgtggatacc aacatgtaaa ggccagcggc tgtttctgtt
    51121 tcgcaataaa tttgtccaca gcggcagtgg caaaaacgag atttttttaa tctgttccgg
    51181 caccgacatt accgaagagc gccgcgctca ggagcgactg cgtattctgg caaataccga
    51241 cagtatcacc ggactgccga atcgtaacgc aatgcaggat ttaatcgatc acgctattaa
    51301 tcatgcagat aacaataaag ttggggttgt gtatcttgat ttggataatt tcaaaaaggt
    51361 caacgacgcc tatgggcatt tgtttggtga ccagttatta cgcgacgtgt cattggctat
    51421 tttaagctgt ctcgaacatg accaggtgtt ggcgcgtcca ggtggggatg agtttctggt
    51481 actggcatcc aacacctcac aaagcgcgct ggaagcaatg gcatcacgaa ttttgacccg
    51541 cttacggctc ccctttcgca ttggtttaat tgaagtttat accagctgtt cagtaggtat
    51601 tgcactctct cccgaacatg gttcagacag cacggctatt attcgtcacg ccgacacagc
    51661 aatgtacaca gcgaaggaag gcggacgagg acaattttgc gtttttaccc cagaaatgaa
    51721 tcaacgggta tttgaatatc tctggctgga taccaacttg cgtaaagcac tggaaaacga
    51781 tcagttggtt attcactatc aaccgaaaat cacctggcgt ggcgaagtgc gcagtctgga
    51841 agcactagta cgttggcagt cacctgaacg tgggttgatt ccaccgttgg acttcatttc
    51901 ctacgccgaa gagtcagggc taattgtgcc tttaggccgt tgggtgattc tcgatgtcgt
    51961 acgccaggtg gcaaagtggc gggaaaaagg cataaacctg cgagtggcgg taaatatttc
    52021 tgcacgtcag ctcgccgatc aaaccatttt caccgccctg aaacaggttc tccaggaact
    52081 caattttgaa tactgcccta tagatgttga actgacagag agttgtctga ttgagaatga
    52141 tgaactggca ctgtctgtta ttcaacaatt tagccaacta ggtgcgcaag tgcatctgga
    52201 tgattttggt accggctact cttcactttc gcaactggcg cgctttccga tcgatgccat
    52261 caaacttgac caggtttttg ttcgagatat tcacaaacaa cctgtctcgc agtcactggt
    52321 ccgggcgatc gtcgctgtgg cccaggcatt gaatcttcag gtgatcgccg aaggtgtaga
    52381 gagtgcaaag gaagatgctt ttttaaccaa gaacgggatc aatgagcggc aaggattttt
    52441 gtttgccaaa ccgatgcccg ccgtcgcctt cgaacgctgg tataaacgct atctgaagcg
    52501 cgcataagta gatgaatata tatacttagc gctatatccc gtcattatat atcgtattcc
    52561 tggatgataa ttagccgttg ctgttaaaca cttctctttt agcggcaatt tttgcattat
    52621 tcattttccc ccttttgcta taaggatgtc cttgatgtct gaatttgatg cccagcgcgt
    52681 tgccgaacgt attgatattg tgttagatat ccttgtggca ggcgattacc actctgcgat
    52741 ccataatctc gagatactta aagcagaact cttgcgccag gttgctgaat ctacgccaga
    52801 catcccgaag gcaccgtggg agatttaaca atcgtgcctt caggttttcc tatttgagga
    52861 tgcccggcgc gtcagtattt ttttatttag tattataacg ttataagaat tacagcgatg
    52921 aattcccgac aacaaactat tctacagatg gtcattgacc agggtcaggt tagcgtaacc
    52981 gatctggcaa aagccactgg agtttctgaa gttaccattc gccaggatct caacaccctc
    53041 gaaaaactga gttacctccg ccgtgcacat ggctttgcag tttcgcttga tagtgatgac
    53101 gtcgaaaccc gtatgatgag caactatacg ctgaagcgtg aactcgccga gtttgccgcg
    53161 tctctggttc aaccgggcga aaccatcttt atcgaaaatg gtagcagtaa tgccctgctc
    53221 gcccggactc tgggcgaaca gaagaaaaat gtcactatca tcacagtcag cagctacatt
    53281 gcgcatttgc taaaagacgc gccttgtgaa gtcattttgc tcggtggcgt gtaccagaaa
    53341 aaaagcgaaa gtatggttgg ccctttgaca cgccagtgca tccagcaggt acatttcagc
    53401 aaagcattta ttggtattga tggctggcaa cctgaaactg gattcaccgg tcgcgatatg
    53461 atgcgtaccg atgtggtcaa tgccgtgctg gaaaaagagt gcgaagcgat agtcctgact
    53521 gacagctcga aatttggtgc tgtacattca tactccatcg gtcccgttga gcgattcaat
    53581 cgcgtgatta ccgattcgaa aatacgcgcc agcgatctga tgcatcttga gcacagtaaa
    53641 ctcaccgttc acgtcgttga catttaacca tttcccttct ctacggatga tttgcagttt
    53701 ggcaaatcat ccgctctaag atgattcctg gttgataatt aagactattt acctgttatt
    53761 aacacgctta agatataaaa ttattatcag cgatataaca ggaagtcatt atcacctgcg
    53821 tgatataacc ctgcgcgcga gcagatttca cggaataatt tcaccaggct tattcttagc
    53881 tattatagtt atagagagct tacttccgtg aatcataaat tcaggagaga gtattatgtt
    53941 tgtaacgagc aaaaaaatga ccgcggctgt tctggcaatt actttggcaa tgtctctgag
    54001 tgcctgttct aactggtcta aacgggaccg caacaccgca atcggcgcgg gtgcaggggc
    54061 attaggcggt gcagtactga ccgatggcag tacgttgggt acattaggtg gtgcagctgt
    54121 cggtggtgtt attggtcacc aggtcggtaa ataatcgtaa ataaatgtcg attatcgact
    54181 cgcaattata atacgcttga ttctgttcag tacgaaataa ctgaataata cacacagtaa
    54241 aatattcgaa agccacggat atatccgtgg ctttttatgt taaccgcctg cgagttttac
    54301 cttcatccct ttcgcttcaa gtagtgattt taataaatca cgcttatcgc cctggatttc
    54361 aataactcca tctttaactg ctccgccgca gccgcatttt ttcttcagtt ccgctgcaag
    54421 ttttgtcagt tcggcatcat cgagatcgac accggtaatc aggcaaacgc ccttaccttt
    54481 acgtccactg gtctgacgct gaatacgcac cacaccgtcg cctttagggc gtacaggggc
    54541 cgctttgggc tcatcaatac gtccggtttc cgttgagtag accagacggc tgttggaatc
    54601 actcatcatg cactccgctg taaagaggcg ttgatcgctt tcagcgtctg cgctggatct
    54661 accgattgcg ttaccgggcg accaatcacc atataatcaa caccagccga caacgcctgt
    54721 tctggcgtca taatgcggcg ctggtcacca gcttcactcc cctgcggacg aatgcccggc
    54781 gtaaccagtt tgaactcctg accgaatacc tgtttaaagc gcacagcttc ctgagcagaa
    54841 cacaccacac catcaaggcc acatttttgc gtcagtgccg ccagacgttc tgcataatct
    54901 gcaggtgaca gtgtcatgcc aagatcgacc aggtcgctgg cttccatgct ggtcaacact
    54961 gtcacagcaa tcaaaagcgg tgcatctttg ccaaacggaa ccagtgcctc acgcgctgcg
    55021 gtcatcatac gcgccccacc agaggcatga acattcacca tccacacgcc taagtcagct
    55081 gcagcagcga cagcgtgcgc agcagtgtta ggaatatcgt ggaatttcag gtcaagaaag
    55141 atatcaaaac cacgctgttg aagttcgcgc acaaactgtg gcccaaacaa tgtaaacatc
    55201 tctttgccga ccttcagacg acaatcgcgt gggtcgatct tgtcgacaaa ggacagcgcg
    55261 tcatcacgat tatgataatc aagggcaaca accacaggag aattcgtaac agcgcgggaa
    55321 gaagatgaag cagttaacgt catgaccaga ccttcttgat gatgggcacc ggaagtggcg
    55381 cgaaacaggt aaacggcgag cattctaccc gtggaaataa aaaattaaca gttgcgattt
    55441 ttcccctggc aggctttttc tgcgccgtcg ttgccggagc acaatggaac atcaataact
    55501 agtatgttgt aactaaagta ggatttttta aaaattacag gccatcaaga ccgcgaatcg
    55561 gtttaatggt tgaccaggcc cgacaagacg gacaatgcca gtagagggtg tatgcggtaa
    55621 aaccacattt ctggcagcga taacgaggct tactacgtac cttttcgcca accatgtcac
    55681 gcagcaccat caggctctct ttggcacgcc cttcttccgc ttcatttaag tggtaatcca
    55741 ttaacttatg gaacacacgc atggtcggat gacgctgaag ctggcgcgta atatagacct
    55801 gtgcggcctc actaccgtcg cgcgcttcga tgatatcagc aagcatcaat tcagcatcgg
    55861 caccggtgtt ctcttccacc gcgcgttgca ggaattctgc ccattcggca gttttaccca
    55921 actgctgata gcaggtttgc aacatttcca gcgtttcgct gaccagttct ctgtcctggg
    55981 atatcacgcg ttgcaaactt tcgacggctt tggcgtattc tcctttcgcc ataaacacgc
    56041 gtcccatcat gatggatacg cgggcgctgt ttttatctgc cgccgcccct ttttttagca
    56101 aggtcatggc acgatcgaga tcgtcgctgg ccatatgctg cagggctaac tcacagtaga
    56161 aatgggcaat ttcgacgcgc tgtttatctt tacccagctt caccaggcgt tcggcaacat
    56221 caattgcttt ctgccactca ctggtagcct ggtagatttg tagcaactgt tgcagagcgc
    56281 caatgcggaa gtcagtttca tcggtcagct gattgaacat gtcttccgcg cggtcgtata
    56341 acccggcggc catgtaatca cgccctagtt gttgaatcgc caacagacgc tgttcatagg
    56401 tcagcgaggc gctttccatt agggtctgat ggatgcgaat agcgcgatca acttcgccac
    56461 gcgaacggaa caggtttccg agcgtaaggt gggcttcaac ggtgcctgta tcctctttaa
    56521 gcatatcgag aaacaggtct accgctttat cctgttgatt actaagcagg aagttaaccc
    56581 ccgctacgta atcacgcgac aagcggttgg cttcatcttg cttgttttgt tgcgcacttc
    56641 tgcggcccat ataccagcca taggccgcgg ctacaggcaa aagcagaaac aacaactcca
    56701 gcatagaaag ttattccttc gccgctgacg agtgcggcac tacagccacg tcagtcgcgg
    56761 gtgaaagctg gttttccagt cgctttattt tacgttcagc gcgcgccagg gaaacacgaa
    56821 ctcgcagcca gaacaggcca caaatcaacc aaccgatagc aaaccccgca gcaaacaata
    56881 ccgccagcaa tgtggaaata cggtactccc cttgcgctaa cagataatta aacgtcacct
    56941 gttgatcgtt ctgcgcaccc aacgtgaccg aaatcacgaa gatcgctaac accagtaaga
    57001 aaatgagtaa atatttcaca ttacttcccg ttatgtggtt agagcgaata aattatggtc
    57061 aacttaccgc aatagccctt taaagtacca ttttcgcgcc gtctgcgaaa tggcatccac
    57121 gcgcattgtt gatgatttaa gggctaaatt cggaaaatca accagcgtta actttcttgt
    57181 tctcgttgcg ctatttcgcg attttcttcc gcaggtggtg ttaatggccc acaaattcgt
    57241 tgcgcaagcc acgttgccac cgccaccagc gcccacgaaa tcaacgtagc tactaccaga
    57301 tcgcgtggcc aatgcatccc gagcagcagg cggcttccca tgactcccgt tgcccagacc
    57361 agcaagatag caatggttaa cgttcgccga cgcggccaca gcaaaccaac ggccagcagt
    57421 gcccaactgg cagcaaacat cgtgtgaccg gaaggaaagg caaaccccgt ctctttctgc
    57481 caatgtgaac gcaaatattg tgggatattt ttctcttcag ccaactgttc tttcactaga
    57541 tttccgcgtt ctgctcgctt taaagtgtag aactcatcaa ccggaatatg atgtgttttt
    57601 tccagccaga taacaaaagg tcgtggttcc tggactttgt ctttgatcca ggatttaacg
    57661 ccttgtccca caaggattgc ggccgccaga atggcaaata atacaaaggc agccttaatg
    57721 cgaaaacgca gacaccagag aaaccagccg aataaaatca aatgtgtaat gacgccccag
    57781 ggctgggtga cagtttcagt aacccaaaaa gccgctttta gtagccaact ttgttctcca
    57841 ggttgccaac gccagccaga aatccatacg gctactggca tgacaagcaa tagtgcagct
    57901 cccactgcgg tacgtctggc aatcgaacgc atggcctctc ctttttgata agtcccacaa
    57961 tcataactga aaacgccagt tccaggaaaa attgacagat ttgtgccatt ccgtgaacga
    58021 tcgacgcgtc gtgattaggt gaaccccttc tcgttatggc aaaataagcc aatacagaac
    58081 cagcattatc tggagaattt catgcagctt aaacgtgtgg cagaagccaa actgccaacc
    58141 ccatggggcg atttcctgat ggtgggattt gaagaactgg caaccggaca cgatcatgtc
    58201 gcgctagtct atggcgatat ttccgggcat accccggtac ttgcgcgcgt ccattccgaa
    58261 tgtctgaccg gtgacgccct gttcagcttg cgctgcgatt gtggcttcca gctcgaagcg
    58321 gcattgacgc aaattgccga ggaaggccgt ggtattttgc tgtatcaccg tcaggaaggt
    58381 cgtaacattg gtctgctgaa taaaatccgc gcttacgcac tgcaggatca aggttacgat
    58441 accgtagagg ctaaccacca gttaggcttc gccgctgatg agcgcgactt cactctttgc
    58501 gctgatatgt tcaaactcct tggcgtcaat gaagtccgct tgttaaccaa taacccgaaa
    58561 aaagtcgaaa ttctgaccga agcagggatt aatattgttg aacgcgtacc attgattgta
    58621 ggtcgtaacc ccaataacga acattatctc gataccaaag ccgagaaaat gggccatttg
    58681 ctgaacaaat aaccctcttg cattgtgtaa ttaatttgct tgccggaagc aaaataaccg
    58741 gcaagcaaat acttgttact tcaacatatt acgaatgaca taatgcaaaa tgccgtcgtt
    58801 ctggtagtag gtcaactccg tcgcggtgtc gatacgacaa cggcagggta cgacctcctg
    58861 gctaccatcc gcgcgcgtaa gcgtcaccgg aaccgtcgca ccgggttgta gattttgcag
    58921 atcgccaata tcaatcttct cttccccggt tagtcctaac gttttacgcg ttacgccttg
    58981 cggaaattcc agcggcagga tgcccatgcc aattaaattc gaacggtgaa ttcgttcaaa
    59041 cgattcggca atcaccacac gaattccaag cagacgagga ccttttgccg cccagtcacg
    59101 gcttgagcct gatccatact ctttcccggc aatcaccgcc agcggcgttt gttccttctt
    59161 atagcgcatc gcagcatcat aaatagagac tacgtcgctg tcaggtaaat gccgcgtcat
    59221 ccccccttca acgccaggca ccatttcatt acggatgcga atattggcga aggtgccgcg
    59281 catcatcact tcatggttac cacgccgcga accgtaggag ttaaagtctt ttcgctcaac
    59341 accccgacct tgtagatatc gacccgctgg gctgtcgggc ttaatactgc ccgccggaga
    59401 gatatggtca gtggtgactg aatcccccag cattgcgagg atccgcgcac cgtgaatatc
    59461 ttccactggt gctggtgttg cctgcatttc atcaaagaaa ggcgataagc gaatataggt
    59521 tgagtcctcc tgccaaccgt aggtatcgga tcgtgtgacg ttaatttcct tccactctgc
    59581 tgtgccttca aaaacttctg cgtactcttt gcggaacatt tctgtggaga cttgttctac
    59641 cgcacgggca atttcttgtg ccgatggcca gatatctttc agataaaccg ggtcgccttt
    59701 acgatcatga ccaataggct cagcagccag gttgatattc atatttcccg ccagcgcata
    59761 ggcaaccacc agcggcggcg aggccagcca gttagtttta accagcggat ggatacggcc
    59821 ttcaaagtta cggttgccgg acagcaccgc accgacggtt aaatcgcctt ttttgattgc
    59881 cgtttcgata ggatcgggca gcggcccaga gttaccaata caggtggtac aaccgtatcc
    59941 cacaaggtta aaccccagtt cgtcgagata cggtgtcagt ttcgcttttg ccagataatc
    60001 agaaacgact ttcgaacccg gtgccagcga cgctttgacc cacggttgcc gcttgaggcc
    60061 cagagttacg gcttttttcg ccagcaagcc tgcggccatc agcacacttg ggttagaggt
    60121 gttggtgcac gaggttatcg cagcaatgac cacagcgcca tcaggtaact gatactgatg
    60181 tccgttcata acataatcga ccggctggcg atctttatgc gtggcattca cttccagttc
    60241 gttactggcg gcaaatgctt ttggtacatc gggcagtgca acgcgatcct gtgggcgttt
    60301 tggccctgcc aggctcgctt caacgtcatt catatccagt tctaacgtac tggtaaaaat
    60361 cggttcatcg cccgggttac gccacatgcc ctgcgctttg gcatattttt cgaccaactc
    60421 aacctgatct tcgctgcgcc cgcttaaacg catgtaatcg agggttacag catcgattgg
    60481 gaagaagcca caggtggcac catattctgg cgacatattg gcaatggtgg cgcgatccgc
    60541 cagcggtagt gaatccagac catcaccata aaattcgacg aatttcccca ccacgccatg
    60601 tttgcgcagc atttgggtaa cagtgagaac caggtctgtg gcggtaatac cttcacgtaa
    60661 tttaccggta agtttaaagc ccactacgtc cgggataagc atggaaaccg gctggcctaa
    60721 cattgcggct tctgcttcga tcccacccac gccccacccc agcacgccaa ggccgttgat
    60781 catagtggtg tgcgagtcag taccaacgag tgtatccgga taagcaatcc attcaccgtc
    60841 ctgcaattca ctccacactg ctttgccgag atattcgagg ttaacctgat ggcaaatgcc
    60901 tgtgcctggc ggcacgacgc taaaccgact gaacgcttgc tttccccatt tcaggaacac
    60961 ataacgttcg tggttgcgct ccatttccag gcgtacgttt tcttcaaatg cctcatcatc
    61021 accaaaacga tcgacggtca ccgagtggtc aatgaccagg tcgaccggtg agagcgggtt
    61081 aacctttgca gtatcgccgc cgaggcgttt aaccgcttcg cgcattgccg ccagatcaac
    61141 aacggcaggt acgccggtaa agtcctgcat cagcaccctt gccgggcggt aggcaatttc
    61201 acggtcagca tgggcatttt tcagccatcc tgccagcgcg tggatatcct cttcggtaac
    61261 cgagttacca tcctgccagc gcagcaggtt ttcgagcaaa actttgagtg acttgggtag
    61321 acgggtgata tcgcccagtg atttagcagc aagcggcagg ctgtagtagt ggtaagtttt
    61381 atctttggcc tgcaacgtgt ccttactggc ttctcgtagg gttgacgaca tagctcctcc
    61441 ttaatgacag ggttgcgaag ccctgattct cttcagggtg aatattaaag ataacacaaa
    61501 catcgcgtaa cgatttgata acaacccaaa ttgataaaag aggagaaacc ttgtggcaaa
    61561 gcaaatgaca accccgccgc agcggggtca aggatcagtg agtaatgatc cagaggagtt
    61621 cggcccagaa gagcatcgac agagaaaacg cgcccatcca ggaccaatat ttcatatttg
    61681 tgttgatcat aggctacacc cttaacggtt attattttgt gttgagataa ctcaagctgt
    61741 tattgctgcg gtgtattaaa agccctttaa ttcattaaaa atgggtaata agaatgatga
    61801 taattattgt ttcggctgat catcttcagc aaacagttct ataaaggctt gctgctgcga
    61861 tgttagttgc cagtcgtctg gaataacgac ggaacattcc cgtttcgttt ctgtttcatt
    61921 gtcactatgc tggcataatg gtgacgactc cagttgctcc tgaccgacca tagttatccc
    61981 cacactttcc aaatcagcag tgcgacaacc acccagaaaa gtgcagaacc aagaaaaacc
    62041 gccagccagg ctttacgttt taattcagga tcgcggcggg gttcctgatt tccagaaggc
    62101 attgctaacc tcatataatc gccatcactt atcagcaaga cgttgaacga ttggtgtaat
    62161 taatcatacg atgagacaaa tcctaaagaa actttagtca aaaatccagc gaatttacga
    62221 atcgtctcca gtgaataatt caatcttttg acctgaaata gatatttgaa aactggagtt
    62281 tgcgaggcgg gtaattagac actactttac aggtaattta ccctataaat atgagggata
    62341 aaaatattgt gcacggtata gaaataagta acaaaagaga atatattccg gcaccttcgc
    62401 tacataaaag gtgccgaaaa taacgcaaga aattattttt ccggcagttt tatatcttta
    62461 aacatgacct caatttcttc attagagcgc aatgcgacag ccgcatcaac gacatcacgc
    62521 gttaaatgcg gtgcaaaacg ctgaatgaaa tcatacatat aactgcgcaa gaaagtacta
    62581 cggcgaaaac caattttggt tgtactgtgg ctgaagatat cgtgagcatc aacacgcaca
    62641 aggtcgggat cggcgaccgg atccaccgcc atgctggcaa tgacccctac ccccagccct
    62701 aaccggacgt aagttttaat gacgtcagca tccgttgccg tgaagacgat acgcggcgtt
    62761 aaccctgcgc ggttaaaggc agtatccagt tctgaacgtc cggtaaagcc gaaggtatat
    62821 gtcaccaacg gatattgcgc cagttcttca atggtaatgg cttttttgcc tgccagcggg
    62881 tgatccggag tgactacaat agcccgattc cagtggtagc acggtaacat cactaaatct
    62941 tcatacagat gcagcgcttc tgtggcgata gcgaaatcag cattgccttt agagacggca
    63001 tcagcaattt gtgtcggcga gccctggtgc atatgcaaag aaacgcgggg ataacgctca
    63061 ataaagcctt tgataacgtt tggtaacgcg tagcgtgcct gggtatgcgt ggtggcgata
    63121 tacagcgagc ctttatccgg ccaggtgtgc tcaccggcaa ccgattttat ggcatcgact
    63181 ttcgacagga cttcgcgagc gatacgaatt atttcttgtc ctgctggcgt tacctgcgtc
    63241 aggtgcttgc cgcttcggga aaaaatttga atgcctagct cgtcttccag cattctgact
    63301 tgtttactga tcccgggttg tgatgtgtaa agtccttccg ctgttgatga gacattcagg
    63361 ttatgattga ccacctcaac aatatagcga agttgttgta atttcatgtt aaaccatcca
    63421 cttagacgcc atcagaacat cgttttttcg tcacacctgg cgggtgacta atacgattta
    63481 ataataaaaa aggtgctaac tataaccact atatcattta tagccttagt gtataggtgt
    63541 gaagaaaaaa taatatgcaa taaaaaaggg ccgctttcgc gaccctttgt ttataaaaac
    63601 ctgacagaat taaaggttat ttttttcctt caacccattt gccatcaaca taaaatgctg
    63661 accagccagt cgcctttccg tctttttccg aagagacata ttgctgtttg gttttacggc
    63721 taaagcgaac catggtctta ttaccttccg gatcctgctg tggcgcatcg gccagataac
    63781 gcagtttttc cggcagacgg tcgcggaagc gataaagctc ttccaccagt ggcgcacgcg
    63841 tttcacgcga tttcgggaaa gtgttggcag ccaggaacac accggcagca ccgtcacgca
    63901 gcacgaaata agcatctgat ttttcgcacg gcagctcagg taatggcacc ggatcttctt
    63961 tcggtggtgc cacttcgccg ttacgtaaaa tcttacgtgt gtttttacac tcttcgttgg
    64021 tgcaggccat gtatttaccg aaacgcccca ttttcaggtg catttcagag ccacattttt
    64081 cacactcaac gatcgggccg tcataacctt taatgcggaa ttcgccctct tcgatctcgt
    64141 aaccgtcgca ggttgggtta ttaccacaga catgcaactt acgtttcgga tcgatgagat
    64201 agctgtccat cgccgtgccg catttcgggc aacgacgttt tgcgcgcagc gcgttggttt
    64261 cagcatcttc gccttccagc acgttcagca cttcgttttc cggcaccagg ttaatggtgg
    64321 ttttgcaacg ctctttcggc ggcagcgcat agccagaaca gccaaggaat accccggtgc
    64381 tcgctgtgcg aatccccatt ttgcgaccac aagtcgggca gtcaatgctg gtcagaacca
    64441 tctggttcgg gcgcatacca ccctcttccg gatctttttc agctttatct aactgctggg
    64501 tgaaatccga gaagaagtga tccagtacag ctttccactc tgcttcgtga tttgccacct
    64561 ggtcgaggct gttttccatc tgcgcggtaa agtcgtagtt cattaactcg cggaaatttt
    64621 cttcaaggcg atcggtgacg atttcgccca ttttttccgc atagaaacga cgattttcta
    64681 ctcgcacgta gccacgatcc tgaatggtcg aaatgatcga cgcataagta gacggacgac
    64741 cgataccacg tttttccagc tctttaacca gcgatgcttc actgaaacgg gctggcggct
    64801 tggtaaagtg ctgggctggt gtaagttcaa cgagcgtcag agcatcgcct ttattaactg
    64861 ctggtaagat gcgatcttca tcgcctttac gcaacgcagg catcactttt gtccagccat
    64921 caaaacgcaa aatacgaccg cgtgctttca ggcggaaatc gcccgcacca acggtcagcg
    64981 tcgtggagtc atatttcgct ggggtcatct ggcaggctac gaactgacgc cagattaact
    65041 ggtacagttt ctgcgcatct gcttccatat ccttcagcga ttccgccatc acattgacgt
    65101 cagaaggacg aatcgcttcg tgcgcttcct gtgagttttc tttgctggcg tactgattcg
    65161 gactttccgg cagatatttc ttaccaaaat tatcgctgat ataaccgcga accatattta
    65221 ccgcgtcctg actcaggtta gtggagtcgg tacgcatgta agtgatatag cctgcttcat
    65281 acaaacgctg cgccatcatc atggtttttt tcacgccaaa tccaagacgg gtgctggcag
    65341 cttgttgcag cgtagaggta ataaaaggag cgccaggttt actggttgtc ggtttgtctt
    65401 cacgttccag cacgctgtag cgcgcttttt ccagcagact taccgcagcc tgagtttgtt
    65461 ctttgttgac cggacggaac ggtttgtcgt tctgatgagt cacctgtaac gccaacgctt
    65521 caccagatgg cgtggtcgtg ctggcatcga cttcccagaa ctcttccggc acgaacgctt
    65581 taatttcacg ctcacgctcg accaccaggc gaaccgccac cgactgcaca cgaccggcag
    65641 acagaccacg agcgatcttt ttccatagca gcggcgaaac catatacccc accacgcggt
    65701 ccataaagcg acgcgcctgc tgggcattaa cacgatcaat attcagctca cccggtttat
    65761 taaatgcctg gcggatcgcg tttttagtaa tttcgttaaa caccactcgg ctatagcgcg
    65821 catcatcacc cccaatcact tcccgcaggt gccatgcaat ggcttcccct tcgcggtcaa
    65881 ggtcggttgc gagatagatg tggtcggctt tttcagccag ttgtttcagt tcagagacga
    65941 ccttctcttt accaggcaac acttcatagt gcgcctccca attgtgccac gggtcaaccc
    66001 ccatacggtt gacgagagcg ccacgttcat cctttttagg ctttttagcc gtcttggtgg
    66061 aggtagagtc ggcactcttt ttggcagctg agccactggt cggcaaatcg cggatgtgac
    66121 cgacgctgga tttcaccacg tagtcactac ccagatactt gttgatcgtt ttggcttttg
    66181 ccggggactc aacgatgaca agagctttac ccatattcac cttacctaat ttgattcttc
    66241 caggaatgcg tcgcacgttg ataaaccttc cactggcaac gagttaccga tatggactcg
    66301 ttctctgcgg atatcaaccc cttgtcgtga ctctgttcac gaaaattcag gtgactgagt
    66361 taacaggtct tggcctacag gcgctatagc acgacggaat tttggtcgaa tgtcaagcaa
    66421 atctattgcc agattgacga aagcgtcaca ctgtacctga taaaatccga tacgcaactt
    66481 tattagcatg caaaatccaa agttaccagt aaggcgctct tgccaccagc gtggtatatt
    66541 tgtctgcaag ccatgcctgg cgtagactaa tgtccttgtt taaggagtaa ataatgaaca
    66601 aagagacaca acccatcgac cgcgaaacgc tgttgaaaga ggccaacaaa atcattcgtg
    66661 agcatgagga tacgctcgca gggattgaag ccaccggcgt cacgcagcgt aatggcgtgc
    66721 tggtatttac cggcgactac tttttagatg aacaaggatt gccaacagcc aaaagcaccg
    66781 cggtgtttaa tatgttcaaa cacctggccc atgtgctttc agaaaaatat catctggttg
    66841 attaaagcaa aacgcgaggt cttagcctcg cgtttgtctt ttacatcaat ggcttttgac
    66901 cccgctgcca ccagcgtagc aacaatcgat cggcgctctc tgccgcgctg ccggtgaatc
    66961 ggtcaatgag tcgtttacgc tgcatatagc gtacattaac cacttcacgg ccttccatca
    67021 ggctaagaat aacttcatca ctggtgttga tttcatcaac caggcctttc tctaccgcct
    67081 gttgtccgta ccagtgttca cccgttgcca cctgttcaat atccagagac ggacgcatac
    67141 gcttcacaaa atctttaaac aactgatgcg tttcgttcaa ctcttcgcgg aatttctccc
    67201 gcccttcttc ggtattttca cccagcaagg tcagagtacg cttatactgc ccggcagtgt
    67261 gcagttcgat atcaatatct ttgcttttca ggaagcggtt aaagttgggc atttgcgcca
    67321 ccaccccaat ggaacccaca atagcaaacg gtgcggaaac aattttgtcc gccacacagg
    67381 ccatcatgta accgccgctg gcagcgactt tgtctaccgt aacagttaaa ggaatgtttt
    67441 tatcacgcag acgctgcagc tgcgaagccg ccaacccgta accatgcacc atgccaccag
    67501 ggctttccag acggagcaca acctgatcct gcggtttgaa tgctgcgagt accgccgtta
    67561 tctcttcacg tagcgagttc acttcatggg cgtccatgct gcctttaaaa tccagcaccc
    67621 agacgcgagg tttactgtca gttgccacct cgcccagttt ggctttcgct tttgctgctt
    67681 tcgcttcttg cttgtgcttt ttcttctgcg ctttgtgcca ctgtttttgc tgatgtgagt
    67741 ccatcagcgc cgcggccagt tcttctttca tctccttata ctgttcgctg agattgttga
    67801 cccgtaactc gccacgctgg cgtttattac gttgagcaac attgacaata atggcggcaa
    67861 tcgccgcaat cgctagcaca acggtaacga ttttcgccaa aaacaaacca tattcagaca
    67921 acaattccac gagtccccac cttggttaaa caacgctttg gctcacagtt tacaacaggc
    67981 atgttaagcc gtctcgcaga gatgttattg cagcgaaagt atgacaaagt atccattttg
    68041 cggtgttatc gccccaggct gattgaaatg cccttctgtt tcaggcataa agccccaaag
    68101 tcataaagta cactggcagc gcgacagaca acgtcgttga ggagtagcca tgcattacca
    68161 gccaaaacaa gatttactca atgatcgcat tatcctggtg acgggagcca gcgatgggat
    68221 tggccgtgaa gccgcgatga cgtatgcacg ctatggtgcg acagtgattc tgttgggccg
    68281 taatgaagaa aaattacgtc aggtagccag ccacataaac gaagaaactg gacgtcagcc
    68341 acagtggttt attctcgatt tgctgacctg cacgtccgaa gattgccaac aactggcaca
    68401 gcgcattacc gttaattatc cgcgtctgga tggtgttttg cataatgccg gattgctcgg
    68461 cgatgtttgc ccaatgagcg aacaaaatcc gcaggtctgg caggacgtca tgcaggtcaa
    68521 cgttaatgcc acctttttgc tcacccaggc gctgcttcct ttattactca aatcggacgc
    68581 cggttcactg gtctttactt catcaagcgt tggacgtcag ggacgagcta actggggtgc
    68641 atatgcagcg tcgaaatttg ccaccgaagg gatgatgcag gtactggccg atgaatatca
    68701 gcagcgcctg cgcgtcaact gcattaaccc aggcggtacg cgcaccgcaa tgcgtgccag
    68761 cgccttcccg accgaagatc cacagaaact taaaacaccc gctgatatca tgccgctcta
    68821 cctctggctg atgggcgatg acagccgccg taaaaccggc atgacctttg acgcccaacc
    68881 gggccgtaaa ccaggaattt cccaatgagt gatgaacgct accaacagcg tcagcagcga
    68941 gtgaaagaaa aagtagatgc tcgtgtggcc caggcccagg atgaacgcgg tattatcatc
    69001 gtctttaccg gcaatggaaa aggcaaaacc accgcggcat ttggtacggc aacacgcgca
    69061 gttggtcacg gaaaaaaagt aggtgtcgtg cagtttatta aaggcacctg gcctaatggc
    69121 gaacgcaatc tgctggagcc acatggcgtt gagtttcagg tgatggcaac gggctttacc
    69181 tgggatacac aaaaccgcga gtctgatacc gccgcctgcc gcgaagtctg gcaacatgca
    69241 aagcggatgc ttgctgattc ctcactggat atggttttgc ttgatgaact gacgtatatg
    69301 gtggcgtatg actatttgcc actggaagaa gtggtgcagg cgttaaatga acgtccacat
    69361 caacagacgg tgattatcac gggtcgtggt tgtcatcggg atattcttga actggcagat
    69421 acggtaagtg aattacgccc cgtcaaacat gcgtttgatg ccggtgtaaa agcgcagata
    69481 gggattgatt actaatctgt aaaaatgccg gatatgacgt ccggcatact ttgattaacc
    69541 gttattacgt ccgccagaac gacgaccgcc gctcacctga ctgtgacgtt tcaccgcacg
    69601 acgaatctga ttcgccttca tgcgacgacg gtctttttct acagcgactt tagagctggt
    69661 ttctggcggt agctccacca gttcgcgcag atagttagtc tgggcgaggt ccagctccgt
    69721 ccagccaccg cgtggcagac cttttggcag tgggatatca ccgtaacgaa cacggatcag
    69781 gcggctcacc tgcacaccaa ccgcttccca cagacgacga acttcacggt tacggccttc
    69841 ggtcagagtc acgttgtacc actggttgat cccttcgccg ccgctgaact tgatggtttt
    69901 aaaagctgcc ggaccatctt ccagttgcac gccacgactc aaatcacgca gtttcgcgtc
    69961 gtcaacctga ccaaatacac gcacggcgta ttcacgttca acttcacggc ttggatgcat
    70021 taaacggttt gccagttcac catcggtggt gaacagcagc agaccacagg tgttaacgtc
    70081 cagacgacct acggcaatcc agcgtgcgcc acgcagcttt ggcagacggt caaacaccgt
    70141 cggacgccct tccggatcgt tacgggtaca caactcacct tccggtttgt aataggccag
    70201 aacgcgacaa atttgttcag cggactcacg taccgaaatc agatgaccat cgatacgaat
    70261 tttcagccca ggggtaactt caacgcgatc gccgagttta gcaattttgc catcaacact
    70321 cacacgaccg gcttcaataa tagattcgat ttcacggcgg gagccgtggc cagcgcgggc
    70381 cagcactttc tgtaattttt cgctcataga gcttcctttt agtgtcgcct tcacaggcgt
    70441 cgaacaggag aatcaatggc gccgtatagc gccattttaa ggccgcgtag tatacagagt
    70501 tacgcactta taagaaaggc ttcacatcac ctacgccttc acgcaccacg accggcttat
    70561 catcggtaag atcaataacc gttgtcggtt tctgcccgag atagccgccg tgaataatca
    70621 aatctacctg tttttccaga cggtctttaa tttcttcggg atcggattcg gtaaattcgc
    70681 tgcctggcag cattagcgaa gtggaaagca tcggttcgcc cagtgcttca agcaacgcct
    70741 gcgcaatcgg gttagaaggc acacgcatac cgatggtttt gcgtttttcc tgcaacagac
    70801 ggcgtggcac ttccttcgtc cccttcaaga tgaaggtata gttgcccggc gtgttgtttt
    70861 tcattaaacg aaacgcaacg ttatcaacaa atgaataggt cgagagttca gaaagatcgc
    70921 gacacatcag ggtaaagttg tgaccgtccg gcagctggcg aatacgacaa atacgctcca
    70981 tcgcgttttt atcttcaatt ttacagccga gcgcatagcc ggaatcagtt ggataaacaa
    71041 tcaccccgcc tttacgcacg atctccaccg cctggttgat cagacgttgc tgtgggttat
    71101 caggatgaat ataaaaaaac tggctcataa ttccctctct gtggtgttct gcggctgttc
    71161 ccatagctgc cagacgcctt caacgcctgc gggcagccag agtttacgac ccagttcgat
    71221 ccacgggcat ggctgatgaa aatcagatcc ttgtgatgcc cataaatgat gctgacgcgc
    71281 aagggccgcc agctgggtac gttcattggg cgattgctgg cactgcgcga cttccatcgc
    71341 gtcaccgtgg tgttcggcaa aatgcgcaac caatcttttc agccatttag cactaagatt
    71401 gtaccgccca ggatgagcta gcaccgcctt accgccagaa tgatgaatga catcaatagc
    71461 ttgttctatt gtacaccact gtggcggaac gtatccggtt ttcccgcgcg ccagatactt
    71521 tttaaagaca tccgccattg aactggcttt gccgcactca acaaggaaac gcgcaaaatg
    71581 accgcgcgtc actgcgccgc cctgcgccag tcgttgtgcg ccttccagcg ccccaggtat
    71641 ttgcgctttt tcaagtcttt cggcaatcag ctgcgcccgc tgattgcgcc gttctgtctg
    71701 ctgcgcgagg aactcacaca ttagcggatg agtaatatca atattcagcc cgacaatatg
    71761 aatttcatga ttttcccaga ccgtggaaat ttccacgccg ggaataagat tcaacgccag
    71821 tcccgaacgt gaaatttcct ctcttgctgg cgcgattgca gctgtggtgt catggtcggt
    71881 gatcgccagg gtgccgacgc gcatctcgac tgcacggtgc accaatgctt ctggcgtcag
    71941 gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcat aattcgtgtc
    72001 gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata acggttctgg
    72061 caaatattct gaaatgagct attgacaatt aatcatcgaa ctagttaact agtacgcaag
    72121 ttcacgtaaa aagggtatcg acaatgaaag caattttcgt actgaaaggt tggtggcgca
    72181 cttcctgaaa cgggcagtgt attcaccatg cgtaaagcaa tcagataccc agcccgccta
    72241 atgagcgggc ttttttttga acaaaattag agaataacaa tgcaaacaca aaaaccgact
    72301 ctcgaactgc taacctgcga aggcgcttat cgcgacaatc ccaccgcgct ttttcaccag
    72361 ttgtgtgggg atcgtccggc aacgctgctg ctggaatccg cagatatcga cagcaaagat
    72421 gatttaaaaa gcctgctgct ggtagacagt gcgctgcgca ttacagcttt aggtgacact
    72481 gtcacaatcc aggcactttc cggcaacggc gaagccctgc tgacactact ggataacgcc
    72541 ctgcctgcgg gtatggaaaa tgaacaatta ccaaactgcc gtgtgctgcg cttcccccct
    72601 gtcagtccac tgctggatga agacgctcgc ttatgctccc tttcggtttt tgacgctttc
    72661 cgcttattgc agaatatgct gaatgtaccg aaggaagaac gagaagcaat gttcttcggc
    72721 ggcctgttct cttatgacct tgtggcgggg tttgaagatt taccgcaatt gtcagcggaa
    72781 aataactgtc ctgatttctg tttttatctc gctgaaacgc tgatggtgat tgaccatcag
    72841 aaaaaaagca cccgcattca ggccagcctg tttgctccga atgaagaaga aaaacaacgt
    72901 ctcactgctc gcctgaacga tcttcgccag caactgaccg aaaccgcgcc accgctgccg
    72961 gtggtttccg tgccgcatat gcgttgtgaa tgtaaccaga gcgatgaaga gttcggtggc
    73021 gtagtgcgtt tgttgcaaaa agcgattcgc gccggagaaa ttttccaggt ggtgccatct
    73081 cgccgtttct ctctgccctg cccgtcaccg ctggcggcct attacgtgct gaaaaagagt
    73141 aatcccagcc cgtacatgtt ttttatgcag gataatgatt tcaccctgtt tggcgcgtcg
    73201 ccggaaagtt cgctcaagta tgacgccacc agccgccaga ttgagatcta cccgattgcc
    73261 ggaacacgac cgcgcggtcg tcgtgccgat ggttcactgg acagagacct cgacagccgc
    73321 atcgaactgg aaatgcgtac cgatcataaa gagctttctg aacatctgat gctggtggat
    73381 ctcgcccgta atgatctggc acgcatttgc acccccggca gccgctacgt cgccgacctc
    73441 accaaagttg accgttactc ttacgtgatg cacttggtct cccgcgtggt cggtgagctg
    73501 cgccacgatc tcgacgccct gcacgcttac cgcgcctgta tgaatatggg aacgttaagt
    73561 ggtgcgccga aagtacgcgc tatgcagtta attgccgagg cggaaggtcg tcgccgcggc
    73621 agctacggcg gcgcggtagg ttatttcacc gcgcatggcg atctcgacac ctgcattgtg
    73681 atccgctcgg cgctggtgga aaacggtatc gccaccgtgc aagccggtgc tggcgtagtc
    73741 cttgattctg ttccgcagtc ggaagccgac gaaacccgta ataaagcccg cgctgtactg
    73801 cgcgctattg ccaccgcgca tcatgcacag gagactttct gatggctgac attctgctgc
    73861 tcgataatat cgactctttt acgtacaacc tggcagatca gttgcgcagc aatgggcata
    73921 atgtggtgat ttaccgcaac catattccgg cgcagacctt aattgaacgc ctggcgacga
    73981 tgagcaatcc ggtactgatg ctttctcctg gccccggtgt gccgagcgaa gccggttgta
    74041 tgccggaact cctcacccgc ttgcgtggca agctgcccat tattggcatt tgcctcggac
    74101 atcaggcaat tgtcgaagct tacgggggct atgtcggtca ggcgggcgaa attcttcacg
    74161 gtaaagcgtc gagcattgaa catgacggtc aggcgatgtt tgccggatta acaaacccgc
    74221 tgccggtggc gcgttatcac tcgctggttg gcagtaacat tccggccggt ttaaccatca
    74281 acgcccattt taatggcatg gtgatggcag tacgtcacga tgcggatcgc gtttgtggat
    74341 tccagttcca tccggaatcc attctcacca cccagggcgc tcgcctgctg gaacaaacgc
    74401 tggcctgggc gcagcagaaa ctagagccag ccaacacgct gcaaccgatt ctggaaaaac
    74461 tgtatcaggc gcagacgctt agccaacaag aaagccacca gctgttttca gcggtggtgc
    74521 gtggcgagct gaagccggaa caactggcgg cggcgctggt gagcatgaaa attcgcggtg
    74581 agcacccgaa cgagatcgcc ggagcagcaa ccgcgctact ggaaaacgcc gcgccgttcc
    74641 cgcgcccgga ttatctgttt gctgatatcg tcggtactgg cggtgacggc agcaacagta
    74701 tcaatatttc taccgccagt gcgtttgtcg ccgcggcctg tgggctgaaa gtggcgaaac
    74761 acggcaaccg tagcgtctcc agtaaatctg gttcgtccga tctgctggcg gcgttcggta
    74821 ttaatcttga tatgaacgcc gataaatcgc gccaggcgct ggatgagtta ggtgtatgtt
    74881 tcctctttgc gccgaagtat cacaccggat tccgccacgc gatgccggtt cgccagcaac
    74941 tgaaaacccg caccctgttc aatgtgctgg ggccattgat taacccggcg catccgccgc
    75001 tggcgttaat tggtgtttat agtccggaac tggtgctgcc gattgccgaa accttgcgcg
    75061 tgctggggta tcaacgcgcg gcggtggtgc acagcggcgg gatggatgaa gtttcattac
    75121 acgcgccgac aatcgttgcc gagctgcatg acggcgaaat taagagctat caattgaccg
    75181 ctgaagattt tggcctgact ccctaccacc aggagcaact ggcaggcgga acaccggaag
    75241 aaaaccgtga cattttaaca cgcttgttac aaggtaaagg cgacgccgcc catgaagcag
    75301 ccgtcgctgc gaacgtcgcc atgttaatgc gcctgcatgg ccatgaagat ctgcaagcca
    75361 atgcgcaaac cgttcttgag gtactgcgca gtggttccgc ttacgacaga gttaccgcac
    75421 tggcggcacg agggtaaatg atgcaaaccg ttttagcgaa aatcgtcgca gacaaggcga
    75481 tttgggtaga aacccgcaaa cagcagcaac cgctggccag ttttcagaat gaggttcagc
    75541 cgagcacgcg acatttttat gatgcgctac agggtgcgcg cacggcgttt attctggagt
    75601 gcaagaaatc gtcgccgtca aaaggcgtga tccgtgatga tttcgatcca gcacgcattg
    75661 ccgccattta taaacattac gcttcggcaa tttcggtgct gactgatgag aaatattttc
    75721 aggggagctt tgatttcctc cccatcgtca gccaaatcgc cccgcagccg attttatgta
    75781 aagacttcat tatcgatcct taccagatct atctggcgcg ctattaccag gctgatgcct
    75841 gcttattaat gctttcagta ctggatgacg aacaatatcg ccagcttgcc gccgtcgccc
    75901 acagtctgga gatgggtgtg ctgaccgaag tcagtaatga agaggaactg gagcgtgcca
    75961 ttgcattggg ggcaaaggtc gttggcatca acaaccgcga tctgcgtgat ttgtcgattg
    76021 atctcaaccg tacccgcgag cttgcgccga aactggggca caacgtgacg gtaatcagcg
    76081 aatccggcat caatacttac gctcaggtgc gcgagttaag ccacttcgct aacggttttc
    76141 tgattggttc ggcgttgatg gcccatgacg atttgcacgc cgccgtgcgc cgggtgttgc
    76201 tgggtgagaa taaagtatgt ggcctgacgc gtgggcaaga tgctaaagca gcttatgacg
    76261 cgggcgcgat ttacggtggg ttgatttttg ttgcgacatc accgcgttgc gtcaacgttg
    76321 aacaggcgca ggaagtgatg gctgcggcac cgttgcagta tgttggcgtg ttccgcaatc
    76381 acgatattgc cgatgtggtg gacaaagcta aggtgttatc gctggcggca gtgcaactgc
    76441 atggtaatga agaacagctg tatatcgata cgctgcgtga agctctgcca gcacatgttg
    76501 ccatctggaa agcattaagc gtcggtgaaa ccctgcccgc ccgcgagttt cagcacgttg
    76561 ataaatatgt tttagacaac ggccagggtg gaagcgggca acgttttgac tggtcactat
    76621 taaatggtca atcgcttggc aacgttctgc tggcgggggg cttaggcgca gataactgcg
    76681 tggaagcggc acaaaccggc tgcgccggac ttgattttaa ttctgctgta gagtcgcaac
    76741 cgggcatcaa agacgcacgt cttttggcct cggttttcca gacgctgcgc gcatattaag
    76801 gaaaggaaca atgacaacat tacttaaccc ctattttggt gagtttggcg gcatgtacgt
    76861 gccacaaatc ctgatgcctg ctctgcgcca gctggaagaa gcttttgtca gtgcgcaaaa
    76921 agatcctgaa tttcaggctc agttcaacga cctgctgaaa aactatgccg ggcgtccaac
    76981 cgcgctgacc aaatgccaga acattacagc cgggacgaac actacgctgt atctcaagcg
    77041 tgaagatttg ctgcacggcg gcgcgcataa aactaaccag gtgctggggc aggcgttgct
    77101 ggcgaagcgg atgggtaaaa ccgaaatcat cgccgaaacc ggtgccggtc agcatggcgt
    77161 ggcgtcggcc cttgccagcg ccctgctcgg cctgaaatgc cgtatttata tgggtgccaa
    77221 agacgttgaa cgccagtcgc ctaacgtttt tcgtatgcgc ttaatgggtg cggaagtgat
    77281 cccggtgcat agcggttccg cgacgctgaa agatgcctgt aacgaggcgc tgcgcgactg
    77341 gtccggtagt tacgaaaccg cgcactatat gctgggcacc gcagctggcc cgcatcctta
    77401 tccgaccatt gtgcgtgagt ttcagcggat gattggcgaa gaaaccaaag cgcagattct
    77461 ggaaagagaa ggtcgcctgc cggatgccgt tatcgcctgt gttggcggcg gttcgaatgc
    77521 catcggcatg tttgctgatt tcatcaatga aaccaacgtc ggcctgattg gtgtggagcc
    77581 aggtggtcac ggtatcgaaa ctggcgagca cggcgcaccg ctaaaacatg gtcgcgtggg
    77641 tatctatttc ggtatgaaag cgccgatgat gcaaaccgaa gacgggcaga ttgaagaatc
    77701 ttactccatc tccgccggac tggatttccc gtctgtcggc ccacaacacg cgtatcttaa
    77761 cagcactgga cgcgctgatt acgtgtctat taccgatgat gaagcccttg aagccttcaa
    77821 aacgctgtgc ctgcacgaag ggatcatccc ggcgctggaa tcctcccacg ccctggccca
    77881 tgcgttgaaa atgatgcgcg aaaacccgga taaagagcag ctactggtgg ttaacctttc
    77941 cggtcgcggc gataaagaca tcttcaccgt tcacgatatt ttgaaagcac gaggggaaat
    78001 ctgatggaac gctacgaatc tctgtttgcc cagttgaagg agcgcaaaga aggcgcattc
    78061 gttcctttcg tcacgctcgg tgatccgggc attgagcagt cattgaaaat tatcgatacg
    78121 ctaattgaag ccggtgctga cgcgctggag ttaggtatcc ccttctccga cccactggcg
    78181 gatggcccga cgattcaaaa cgccactctg cgcgcctttg cggcaggtgt gactccggca
    78241 caatgttttg aaatgctggc actgattcgc cagaaacacc cgaccattcc cattggcctg
    78301 ttgatgtatg ccaatctggt gtttaacaaa ggcattgatg agttttatgc ccagtgcgaa
    78361 aaagtcggcg tcgattcggt gctggttgcc gatgtgccag ttgaagagtc cgcgcccttc
    78421 cgccaggccg cgttgcgtca taatgtcgca cctatcttca tctgcccgcc aaatgccgat
    78481 gacgacctgc tgcgccagat agcctcttac ggtcgtggtt acacctattt gctgtcacga
    78541 gcaggcgtga ccggcgcaga aaaccgcgcc gcgttacccc tcaatcatct ggttgcgaag
    78601 ctgaaagagt acaacgctgc acctccattg cagggatttg gtatttccgc cccggatcag
    78661 gtaaaagcag cgattgatgc aggagctgcg ggcgcgattt ctggttcggc cattgttaaa
    78721 atcatcgagc aacatattaa tgagccagag aaaatgctgg cggcactgaa agtttttgta
    78781 caaccgatga aagcggcgac gcgcagttaa tcccacagcc gccagttccg ctggcggcat
    78841 tttaactttc tttaatgaag ccggaaaaat cctaaattca tttaatattt atcttt
//
LOCUS       DAZGJW010000005        71846 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZGJW010000005 DAZGJW010000000
VERSION     DAZGJW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 71846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.005"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            329..1000
                     /locus_tag="ACHKIK_000357"
     CDS             329..1000
                     /locus_tag="ACHKIK_000357"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO9081595.1"
                     /translation="MDKVLDSALPSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            1010..2206
                     /gene="yaiP"
                     /locus_tag="ACHKIK_000358"
     CDS             1010..2206
                     /gene="yaiP"
                     /locus_tag="ACHKIK_000358"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO9081596.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2184..2765
                     /locus_tag="ACHKIK_000359"
     CDS             2184..2765
                     /locus_tag="ACHKIK_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO9081597.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2767..3540
                     /gene="yaiO"
                     /locus_tag="ACHKIK_000360"
     CDS             2767..3540
                     /gene="yaiO"
                     /locus_tag="ACHKIK_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO9081598.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQNFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3726..4001
                     /gene="frmR"
                     /locus_tag="ACHKIK_000361"
     CDS             3726..4001
                     /gene="frmR"
                     /locus_tag="ACHKIK_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO9081599.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSMEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4036..5145
                     /gene="frmA"
                     /locus_tag="ACHKIK_000362"
     CDS             4036..5145
                     /gene="frmA"
                     /locus_tag="ACHKIK_000362"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO9081600.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5238..6071
                     /gene="fghA"
                     /locus_tag="ACHKIK_000363"
     CDS             5238..6071
                     /gene="fghA"
                     /locus_tag="ACHKIK_000363"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO9081601.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6196..6735)
                     /gene="yaiL"
                     /locus_tag="ACHKIK_000364"
     CDS             complement(6196..6735)
                     /gene="yaiL"
                     /locus_tag="ACHKIK_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO9081602.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6837..8048)
                     /gene="mhpT"
                     /locus_tag="ACHKIK_000365"
     CDS             complement(6837..8048)
                     /gene="mhpT"
                     /locus_tag="ACHKIK_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO9081603.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGDKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8320..9333)
                     /gene="mhpE"
                     /locus_tag="ACHKIK_000366"
     CDS             complement(8320..9333)
                     /gene="mhpE"
                     /locus_tag="ACHKIK_000366"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO9081604.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9330..10280)
                     /gene="mhpF"
                     /locus_tag="ACHKIK_000367"
     CDS             complement(9330..10280)
                     /gene="mhpF"
                     /locus_tag="ACHKIK_000367"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO9081605.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ASARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10277..11086)
                     /gene="mhpD"
                     /locus_tag="ACHKIK_000368"
     CDS             complement(10277..11086)
                     /gene="mhpD"
                     /locus_tag="ACHKIK_000368"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO9081606.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11096..11962)
                     /gene="mhpC"
                     /locus_tag="ACHKIK_000369"
     CDS             complement(11096..11962)
                     /gene="mhpC"
                     /locus_tag="ACHKIK_000369"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO9081607.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHQENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11980..12924)
                     /gene="mhpB"
                     /locus_tag="ACHKIK_000370"
     CDS             complement(11980..12924)
                     /gene="mhpB"
                     /locus_tag="ACHKIK_000370"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO9081608.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12926..14590)
                     /gene="mhpA"
                     /locus_tag="ACHKIK_000371"
     CDS             complement(12926..14590)
                     /gene="mhpA"
                     /locus_tag="ACHKIK_000371"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO9081609.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDVL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14781..15614
                     /gene="mhpR"
                     /locus_tag="ACHKIK_000372"
     CDS             14781..15614
                     /gene="mhpR"
                     /locus_tag="ACHKIK_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO9081610.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQD
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15691..16773
                     /gene="lacI"
                     /locus_tag="ACHKIK_000373"
     CDS             15691..16773
                     /gene="lacI"
                     /locus_tag="ACHKIK_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO9081611.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLLINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16896..19970
                     /gene="lacZ"
                     /locus_tag="ACHKIK_000374"
     CDS             16896..19970
                     /gene="lacZ"
                     /locus_tag="ACHKIK_000374"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO9081612.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTFATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWLAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKYQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPGLPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            20022..21275
                     /gene="lacY"
                     /locus_tag="ACHKIK_000375"
     CDS             20022..21275
                     /gene="lacY"
                     /locus_tag="ACHKIK_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO9081613.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(21354..22535)
                     /gene="cynX"
                     /locus_tag="ACHKIK_000376"
     CDS             complement(21354..22535)
                     /gene="cynX"
                     /locus_tag="ACHKIK_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO9081614.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVSWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     V"
     gene            complement(22541..23011)
                     /gene="cynS"
                     /locus_tag="ACHKIK_000377"
     CDS             complement(22541..23011)
                     /gene="cynS"
                     /locus_tag="ACHKIK_000377"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO9081615.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23042..23701)
                     /gene="cynT"
                     /locus_tag="ACHKIK_000378"
     CDS             complement(23042..23701)
                     /gene="cynT"
                     /locus_tag="ACHKIK_000378"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO9081616.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            23811..24710
                     /gene="cynR"
                     /locus_tag="ACHKIK_000379"
     CDS             23811..24710
                     /gene="cynR"
                     /locus_tag="ACHKIK_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO9081617.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(24750..26033)
                     /gene="codA"
                     /locus_tag="ACHKIK_000380"
     CDS             complement(24750..26033)
                     /gene="codA"
                     /locus_tag="ACHKIK_000380"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO9081618.1"
                     /translation="MSNNALQTIINARLPGKEVLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26023..27282)
                     /gene="codB"
                     /locus_tag="ACHKIK_000381"
     CDS             complement(26023..27282)
                     /gene="codB"
                     /locus_tag="ACHKIK_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO9081619.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(27621..29507)
                     /gene="prpE"
                     /locus_tag="ACHKIK_000382"
     CDS             complement(27621..29507)
                     /gene="prpE"
                     /locus_tag="ACHKIK_000382"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO9081620.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(29547..30998)
                     /gene="prpD"
                     /locus_tag="ACHKIK_000383"
     CDS             complement(29547..30998)
                     /gene="prpD"
                     /locus_tag="ACHKIK_000383"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO9081621.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(31032..32201)
                     /gene="prpC"
                     /locus_tag="ACHKIK_000384"
     CDS             complement(31032..32201)
                     /gene="prpC"
                     /locus_tag="ACHKIK_000384"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO9081622.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASYPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIANRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(32246..33136)
                     /gene="prpB"
                     /locus_tag="ACHKIK_000385"
     CDS             complement(32246..33136)
                     /gene="prpB"
                     /locus_tag="ACHKIK_000385"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO9081623.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTIDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEAKLDDLFARSQVK"
     gene            33375..34961
                     /gene="prpR"
                     /locus_tag="ACHKIK_000386"
     CDS             33375..34961
                     /gene="prpR"
                     /locus_tag="ACHKIK_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO9081624.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIHKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRLGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(35059..35334)
                     /gene="yahO"
                     /locus_tag="ACHKIK_000387"
     CDS             complement(35059..35334)
                     /gene="yahO"
                     /locus_tag="ACHKIK_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO9081625.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            35481..36152
                     /gene="yahN"
                     /locus_tag="ACHKIK_000388"
     CDS             35481..36152
                     /gene="yahN"
                     /locus_tag="ACHKIK_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO9081626.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(36169..36414)
                     /gene="yahM"
                     /locus_tag="ACHKIK_000389"
     CDS             complement(36169..36414)
                     /gene="yahM"
                     /locus_tag="ACHKIK_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO9081627.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGQSSPMVGIRQIRLIT"
     gene            complement(36826..37641)
                     /gene="yahL"
                     /locus_tag="ACHKIK_000390"
     CDS             complement(36826..37641)
                     /gene="yahL"
                     /locus_tag="ACHKIK_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO9081628.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(37884..38933)
                     /gene="yahK"
                     /locus_tag="ACHKIK_000391"
     CDS             complement(37884..38933)
                     /gene="yahK"
                     /locus_tag="ACHKIK_000391"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO9081629.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(39020..39976)
                     /locus_tag="ACHKIK_000392"
     CDS             complement(39020..39976)
                     /locus_tag="ACHKIK_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9081630.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            complement(39973..40944)
                     /locus_tag="ACHKIK_000393"
     CDS             complement(39973..40944)
                     /locus_tag="ACHKIK_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9081631.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(40937..42421)
                     /locus_tag="ACHKIK_000394"
     CDS             complement(40937..42421)
                     /locus_tag="ACHKIK_000394"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO9081632.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(42470..43453)
                     /locus_tag="ACHKIK_000395"
     CDS             complement(42470..43453)
                     /locus_tag="ACHKIK_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9081633.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGNAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDSASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(43707..44117)
                     /locus_tag="ACHKIK_000396"
     CDS             complement(43707..44117)
                     /locus_tag="ACHKIK_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO9081634.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(44416..45798)
                     /gene="yahJ"
                     /locus_tag="ACHKIK_000397"
     CDS             complement(44416..45798)
                     /gene="yahJ"
                     /locus_tag="ACHKIK_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO9081635.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(45808..46758)
                     /gene="yahI"
                     /locus_tag="ACHKIK_000398"
     CDS             complement(45808..46758)
                     /gene="yahI"
                     /locus_tag="ACHKIK_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO9081636.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(46901..48319)
                     /gene="yahG"
                     /locus_tag="ACHKIK_000399"
     CDS             complement(46901..48319)
                     /gene="yahG"
                     /locus_tag="ACHKIK_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO9081637.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEKQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(48319..49866)
                     /gene="fdrA"
                     /locus_tag="ACHKIK_000400"
     CDS             complement(48319..49866)
                     /gene="fdrA"
                     /locus_tag="ACHKIK_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO9081638.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(49856..50719)
                     /gene="yahE"
                     /locus_tag="ACHKIK_000401"
     CDS             complement(49856..50719)
                     /gene="yahE"
                     /locus_tag="ACHKIK_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO9081639.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(50759..51364)
                     /gene="yahD"
                     /locus_tag="ACHKIK_000402"
     CDS             complement(50759..51364)
                     /gene="yahD"
                     /locus_tag="ACHKIK_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO9081640.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELAREQGFEEIAQLLIAAGA"
     gene            51622..52119
                     /gene="yahC"
                     /locus_tag="ACHKIK_000403"
     CDS             51622..52119
                     /gene="yahC"
                     /locus_tag="ACHKIK_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO9081641.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            52211..53143
                     /gene="yahB"
                     /locus_tag="ACHKIK_000404"
     CDS             52211..53143
                     /gene="yahB"
                     /locus_tag="ACHKIK_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9081642.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(53185..54273)
                     /gene="pdeL"
                     /locus_tag="ACHKIK_000405"
     CDS             complement(53185..54273)
                     /gene="pdeL"
                     /locus_tag="ACHKIK_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO9081643.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(54415..58398)
                     /gene="ehaA"
                     /locus_tag="ACHKIK_000406"
     CDS             complement(54415..58398)
                     /gene="ehaA"
                     /locus_tag="ACHKIK_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO9081644.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITKNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSAIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSTEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            complement(58971..61004)
                     /gene="betT"
                     /locus_tag="ACHKIK_000407"
     CDS             complement(58971..61004)
                     /gene="betT"
                     /locus_tag="ACHKIK_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO9081645.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            61133..61720
                     /gene="betI"
                     /locus_tag="ACHKIK_000408"
     CDS             61133..61720
                     /gene="betI"
                     /locus_tag="ACHKIK_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO9081646.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            61734..63206
                     /gene="betB"
                     /locus_tag="ACHKIK_000409"
     CDS             61734..63206
                     /gene="betB"
                     /locus_tag="ACHKIK_000409"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO9081647.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGGGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFSPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            63220..64890
                     /gene="betA"
                     /locus_tag="ACHKIK_000410"
     CDS             63220..64890
                     /gene="betA"
                     /locus_tag="ACHKIK_000410"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO9081648.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRI
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            65103..65768
                     /gene="ykgH"
                     /locus_tag="ACHKIK_000411"
     CDS             65103..65768
                     /gene="ykgH"
                     /locus_tag="ACHKIK_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFO9081649.1"
                     /translation="MREQIKQDIDLIEILFYLKKKVRVILFIIAICMAMMLLFLYINK
                     DNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSG"
     gene            complement(66014..66709)
                     /gene="ykgG"
                     /locus_tag="ACHKIK_000412"
     CDS             complement(66014..66709)
                     /gene="ykgG"
                     /locus_tag="ACHKIK_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO9081650.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(66702..68129)
                     /gene="ykgF"
                     /locus_tag="ACHKIK_000413"
     CDS             complement(66702..68129)
                     /gene="ykgF"
                     /locus_tag="ACHKIK_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO9081651.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(68140..68859)
                     /gene="ykgE"
                     /locus_tag="ACHKIK_000414"
     CDS             complement(68140..68859)
                     /gene="ykgE"
                     /locus_tag="ACHKIK_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO9081652.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(69386..70240)
                     /gene="rclR"
                     /locus_tag="ACHKIK_000415"
     CDS             complement(69386..70240)
                     /gene="rclR"
                     /locus_tag="ACHKIK_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO9081653.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            70466..71791
                     /gene="rclA"
                     /locus_tag="ACHKIK_000416"
     CDS             70466..71791
                     /gene="rclA"
                     /locus_tag="ACHKIK_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO9081654.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEANLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT        17436 a        19323 c        17805 g        17282 t
ORIGIN      
        1 tcaatcactc gcatattaca ttcacataat gtatataaac aatacttgtt cacagaacat
       61 attttttaaa tgcaattaat tgacaaacta aatcacatct aaatacaatg tataaaacaa
      121 aataactata catacaaaac atacaaacac ttaacatatg aattgcataa aaacattacc
      181 cagtcgaacc tccgggtctg aaagcccttg cttttcagtt attagcaaca tttaacattt
      241 ataaattaac atctggctgc gccagagatt acgtctctga gtgcatcgtt gtgcttgtag
      301 tataataaat aaaatggagt gtcattaagt ggataaggtt ttagattcag ccctcccttc
      361 ttcggcaaat aaaagaaagg gtatccttgc tattggcgca catcctgacg atatagaatt
      421 aggctgtggc gcatcgcttg ctcgtcttgc gcaaaaagga atttatatcg cagccgtggt
      481 gatgactacc ggcaactctg gtacagatgg aataatcgat cgccatgaag aatcgcgcaa
      541 cgccctaaag atattagggt gccaccaaac tattcatctt aattttgctg acacccgcgc
      601 tcatttacag ctcaatgata tgatttccgc tctggaagac atcattaaaa atcaaattcc
      661 ttctgatgtt gaaatcatgc gggtatatac catgcatgat gccgaccgcc atcaggatca
      721 tctcgctgtt tatcaagctt caatggttgc ctgccgcact attccacaaa ttctcggcta
      781 cgaaacccca agtacctggc tttcatttat gcctcaggtt tttgaatccg ttaaagaaga
      841 atatttcaca gtcaaacttg ctgcattaaa aaaacataaa agccaggaac gacgtgatta
      901 tatgcgtcat gatcgcctgc gtgcagttgc acaatttcgc gggcaacagg tcaatagcga
      961 tctgggtgaa ggctttgtaa ttcataaaat gattctttga gcaaattgca tgaaaacctg
     1021 gatatttatc tgtatgtcta tagcaatgtt gctatggttt ttaagtacgc taagacgtaa
     1081 acccagtcaa aagaaaggct gtattgacgc cattatacct gcgtataacg aaggcccgtg
     1141 tctggcgcag tcactggata atctactgcg taacccttat ttttgccggg taatttgcgt
     1201 taacgacggc tccacggaca ataccgaagc ggtcatggcg gaagtcaaac gcaaatgggg
     1261 cgaccgcttt gttgccgtca cgcaaaaaaa taccggtaaa ggtggtgcgc tgatgaatgg
     1321 cctcaattat gccacctgcg accaggtttt tttaagtgat gccgacacct atgttccgcc
     1381 cgatcaagac ggaatgggct atatgctggc agaaattgag cgcggtgctg atgccgtagg
     1441 cggcattccc tctactgcgt tgaaaggcgc ggggctgtta ccgcacatcc gcgcgaccgt
     1501 aaagttgccg atgattgtta tgaagcgcac gctacagcag ctcctgggcg gcgcaccgtt
     1561 tattatcagc ggtgcctgcg gaatgttccg tactgatgta ttgcgtaagt tcggtttctc
     1621 ggatcgtact aaagtcgaag accttgatct cacctggaca ttagtggcaa acggctaccg
     1681 tattcggcag gcgaatcgct gcatcgtata cccacaggaa tgcaacagcc cgcgtgagga
     1741 gtggcgtcgc tggcggcgtt ggattgtggg atacgcggtc tgtatgcgcc tgcataaaag
     1801 acttttattt agccgcttcg gtatcttcag tatatttcct atgctgttgg ttgtgcttta
     1861 tggcgttggg atttatctca ctacctggtt taatgaattc atcaccaccg ggccgcatgg
     1921 tgtggtgttg gcaatgtttc cgcttatctg gatcggcgta gtttgtgtta ttggtgcttt
     1981 tagcgcctgg tttcatcgtt gctggttgtt ggtgccttta gcgccgcttt ccgttgtgta
     2041 tgtattatta gcttatgcca tctggattat ttatggactt attgcctttt ttactggacg
     2101 cgaacctcag cgcgacaaac ccacccgcta ttccgcactg gtggaagcgt caaccgctta
     2161 ttcccaacct tctgtcacag gaactgaaaa actatctgaa gcttaatgtt aaagagaaaa
     2221 atattcagat tgcagaccag gtaattattg atgaaactgc aggtgaagtt gttatcggcg
     2281 cgaatacccg tatttgtcat ggtgccgtta ttcagggtcc ggtagtgatt ggcgcaaact
     2341 gcctgatagg taattatgcg tttattcgtc ctggcacaat aatcagcaat ggtgtaaaaa
     2401 ttggttttgc caccgaaatt aaaaatgcgg ttattgaagc ggaagcaacg attggtccgc
     2461 aatgttttat tgccgactcg gtagttgcaa accaggcata tttgggcgca caagtacgta
     2521 ccagtaatca tcgtctggat gaacagcctg tgtctgttcg aactccagag ggaattatcg
     2581 ctaccggatg cgataaatta ggttgttata tcgggcagcg ttcacgcctt ggcgtacaag
     2641 ttataatttt gcctgggcga attatttccc cgaacacgca acttggcccg cgcgtgattg
     2701 tagaacgtaa tttacctgcc ggaacttatt cactccgaca agaacttatc cgtacaggag
     2761 attaaaatga taaaacggac gttattagcg gcggccattt ttagcgcatt gcccgcttat
     2821 gccgggttaa cttccattac cgcgggctac gattttaccg attattctgg cgatcatggc
     2881 aaccgtaatt tagcgtatgc tgaactggtg gcgaaagttg aaaacgcaac gctgcttttt
     2941 aatctttccc aggggcgtcg tgattatgaa actgaacatt tcaatgccac tcgcggtcag
     3001 ggtgcggtct ggtataaatg gaataactgg ctgacaaccc gaacgggtat tgcctttgcg
     3061 gataatacgc cggtctttgc ccgccagaat tttcgtcagg atattaacct ggccctgttg
     3121 ccaaaaacgc ttttcacgac cggttatcgc tacactaaat attacgatga tgtcgaagtc
     3181 gatgcctggc aaggcggcgt atcactctat actggcccgg tgatcaccag ctaccgctat
     3241 acccattatg actccagcga tgcaggtggt agttatagca atatgatttc cgtgcgcctg
     3301 aatgacccgc gcggcactgg ttatacgcaa ctatggctaa gccgcggaac aggcgcttac
     3361 acctatgact ggacgccaga aacacgctac ggcagcatga agagcgtcag tctgcaacgt
     3421 attcaaccgc tgactgagca acttaatctc gggctgacgg caggtaaagt gtggtacgac
     3481 accccaaccg atgattataa cggtctgcaa cttgcagccc atctgacctg gaaattctga
     3541 ttccttctgc cgcccgctat ccggggcggc cttccctgcc gattagcccc cccctttcct
     3601 ctttgttttc cgaccacatt caccggataa attttattct ccagtgttat atactatagg
     3661 ggggtatgca ttgacatgta gtataccccc ctatagtata ttgcgtgcag atgatgaggt
     3721 gcgaaatgcc cagtactccg gaagagaaga aaaaggtcct tactcgagtt cgtcgtattc
     3781 gagggcagat tgatgctctg gaacggtcga tggagggtga tgccgaatgc cgtgccatac
     3841 tccaacagat cgctgccgtt cggggcgcgg ctaacgggct gatggcagaa gtgcttgaaa
     3901 gccatatccg ggaaacgttt gaccgaaatg actgctacag ccgcgaagtc agccaatccg
     3961 ttgacgacac tattgaactg gttcgagcct atcttaaata gctgaatcta ttaccatatt
     4021 gaggaagagc gagagatgaa atcacgtgct gccgttgcat ttgctcccgg taaaccgctg
     4081 gaaatcgttg aaattgacgt tgcaccaccg aaaaaaggtg aagtgctgat taaagtcacc
     4141 cataccggcg tttgccatac cgacgcattt accctctccg gggatgaccc ggaaggtgta
     4201 ttcccggtgg ttctcggtca cgaaggggcc ggcgttgtgg ttgaagtcgg tgaaggcgta
     4261 accagcgtca aacctggcga ccatgtgatc ccgctttaca ccgcggagtg cggcgagtgt
     4321 gagttctgtc gttctggcaa aactaacctc tgtgttgcgg ttcgcgaaac ccagggtaaa
     4381 ggcttgatgc cagacggcac cacccgtttt tcttacaacg ggcagccgct ttatcactac
     4441 atggggtgct ctacattcag tgaatacacc gtggtcgcgg aagtgtctct ggccaaaatt
     4501 aatccagaag caaaccatga acacgtctgt ctgctgggct gtggcgtgac caccggtatt
     4561 ggcgcggtgc acaacacagc taaagtccag ccaggtgatt ctgttgccgt gtttggtctt
     4621 ggcgcgattg gtctggcagt ggttcagggc gcgcgtcagg cgaaagcggg acggattatc
     4681 gctatcgata ccaacccgaa gaaattcgat ctggcgcgtc gcttcggtgc taccgactgc
     4741 attaacccga atgactacga caaaccgatt aaagatgtcc tgctggatat caacaaatgg
     4801 ggtatcgacc atacctttga atgcatcggt aacgtcaacg tgatgcgtgc ggcgctggaa
     4861 agtgcgcacc gcggctgggg tcagtcggtg atcatcgggg tggcaggttc tggtcaggaa
     4921 atctccaccc gtccattcca gttggtcact ggtcgcgtat ggaaaggttc cgcgtttggc
     4981 ggcgtgaaag gtcgttccca gttaccgggt atggttgaag atgcgatgaa aggtgatatc
     5041 gatctggaac cgtttgtcac gcataccatg agccttgatg aaattaatga cgccttcgac
     5101 ctgatgcatg aaggcaaatc cattcgaacc gtaattcgtt actgatttcc cgcaggtata
     5161 ccccgtccac ttcagacggg gttcttaata ctctccctgg gcagccgtcc gggggattaa
     5221 ccgtgagata atgactgatg gaactcattg aaaaacatgc cagctttggc ggctggcaaa
     5281 atgtgtatcg gcattattcc caatcactga aatgtgaaat gaatgtcggc gtctatctcc
     5341 caccaaaagc cgcgaatgaa aaattgccgg tgctgtactg gctttcgggc ctgacctgca
     5401 acgagcagaa tttcattact aaatcgggga tgcagcgtta cgcggctgaa cacaacatta
     5461 ttgttgttgc gccggacacc agtccgcgag gcagtcatgt cgcagatgcc gaccgttacg
     5521 atctcgggca aggtgccggg ttttacctga acgcaacgca agcgccgtgg aatgaacatt
     5581 acaaaatgta tgactatatc cgcaacgagc tgccggattt agtgatgcaa cattttccgg
     5641 caacgaccag aaagtctatc tctggtcatt ctatgggcgg gctgggcgcg ctggtgctgg
     5701 cgttacgtaa cccagatgaa tatgtcagcg tctcggcgtt ttcgcccatt gtctccccat
     5761 cgcaagtacc gtggggacag caagcctttg ccgcatatct tggtgaaaat aaagatgcct
     5821 ggttgaatta cgatccggtg agtcttattt cacaaggtca acgcgttgcg gaaatcatgg
     5881 ttgatcaggg gttgagtgat gatttttacg cggaacagct gcggactcca aatcttgaaa
     5941 agatctgcca ggagatgaat atcaagacgt taatccgtta tcacgagggt tacgaccaca
     6001 gctattattt tgtctccagt tttattggcg agcatattgc ctaccacgcc aataaactga
     6061 atatgcgttg ataatagtgc acgactgccg gatgcggcgt gaacgcctta tccgcctaca
     6121 tttcgcccgc aggccgtagg catgataaga cgcgcatagc gtcgcatcag gcatctgcgc
     6181 acgacttatc tatgtttacc acatcaaatc atcaggcact ttgaagtcgg catatgggtc
     6241 gtcttcatcc tgctcttcgg cgctgagcgc gctgtgcaac actatactgc tggcatcacg
     6301 ctgagctatt ttatcggcaa cgctggcggg aataatggcg tattcacctt cgctattgtt
     6361 atcgaccaac aagcgagcaa tcgccagacg accattaatc aactgcgcct gtgtgagctt
     6421 atcgacaaaa atcttcttaa tcagattgcc gtcagtgaag ttaaaaccaa tatcgccatt
     6481 ggcaatggtg attcggttca tttcaatgag ctgcttcacc tgagctttat attctttcgc
     6541 caacgcagct tgtttttgtt gctcgctaag ctgtttatca cgctcaagct gtgccttttt
     6601 attttcttct accgccgccc gagcttcacg cgcctgaacg cgcgattttt tcgccgttct
     6661 ctccaccttc gccgcttttt tgctggtgac taatcctgct ttgagcaact gctcttgtaa
     6721 ggtaagtttt gccatctttg tttccaaacc cgctgaataa tttttagcga gtatacctgt
     6781 aaacgccgtg ggtgtattgg gggcaactcc ctttcgcccc tgcttaataa ggcgcgtcag
     6841 gcatcggcac acggctgcat tcttgatctc cgactcatca aaataaacac cgccaaccca
     6901 gcaacaagaa tacccggtgc agaagcggcc attacgccga ccgtgccagt gcctaatgcc
     6961 agcattttcc cggccagtaa cggaccgctc atagccccca gacgccctac cgccacggct
     7021 gttcccacac ctgttgcgcg gatctgcgaa ctgtaaaaca acggtgccag ggcatacaaa
     7081 acgctttgcc cacctgtcgc aaacaacccc gcgacaaatc ccgccagcaa cataccgtta
     7141 aatgacgaca ccgttccaag tgccagcagc gaagctaaca tgccgctata aatcagtagc
     7201 gacatggtca ctggacgcag cttatccatc aatgcgccca acattaacgt cccgcttgcc
     7261 gcacccattt gcagggcaaa catcacccct gccgcctgcg atggctggaa tccttgctcc
     7321 accaaaagca gcggtagcca gttgatcaac atgtagacca ccagcagagt gaagaaataa
     7381 cacaaccaca gcagcagcgt cgcggttgcc gtttctggcg caaataaggc acgcagtggt
     7441 ggcgcagcct gtttatcgcc agcgaaaacc gccgactccg gcagccagcg cattaatagc
     7501 ggcaccagaa tcaacggcac cacaccacct acccaaaaca ccgtttgcca tgctaagttt
     7561 gcccccgcga aacccagtgt cgccgccagc gccgcgccaa tgggaacacc gcaatacatc
     7621 aggctcactg ctgtcccacg aaaacgtgga cccgcggctt cagacgtcag ggcgataaga
     7681 ttcggcaacg ccgcccccag cccgacaccg gtcatcagcc gcgcaaagac cagtgagggg
     7741 aaatcccagg caatcgccgt tgccagcgag aacaaaccaa acagcgcaac tgagccaatc
     7801 aaaatgcgct tgcgaccata acggtccgcc agcattccgc caaccaacgc gccgggtagc
     7861 aaaccgagta ttccggcgct gaatatccag cccatttgca ttttatcgag tgcgaaagcc
     7921 tgggcgatgc cacccgccgc aatgccagcc gcctgaagat ccagcccttc catcagagcg
     7981 accaaaaaac aaagcccgat agtcagcatc aggcgggatg aagatgatga aggggtacga
     8041 gtcgacataa gatgaacctc gttgcgagtg caggtgaatc gcgactgata cattgccagc
     8101 cgcgtaatca gaactattta atgactgcct acgggcgaaa tgtaggccgg acaagacgtt
     8161 tatcgcatcc ggcagtcgtg tgcagatgcc tgatgcgacg ctaacgcgtc ttatcaggcc
     8221 aaattctcac gccacccgta ggccggataa ggtgtttacg ccgcatccgg cagtcgtgct
     8281 gggatgggca tacaaaatgc acgttctcgg cagtcatcat tatttgttgt tgcgcagatc
     8341 cagcgccacg tcaacgatca tatcctcctg gccgccaacc atccggcgtt tgcccagctc
     8401 aacgagaata tccaccgcac ttaagccata acgcgccgcc gccgtttcac agtgacgcag
     8461 gaagctcgag taaacaccag cgtatcccag cgccagcgtt tcgcgatcga ctcgtaccgg
     8521 tcgatcctgc aacggacgca ccaggtcgtc agcggcatcc attaacgcat agagatcggt
     8581 cccatgctgc cagcccagtt tatccgcggc ggcaataaat acttccagcg gcgcgttacc
     8641 tgcgcccgcg cccattcccg cgaggctggc gtcgattcgg tcgcagccct cttccaccgc
     8701 cgcgatagag ttcgccacgc caagactcag gttatggtga gcgtgcatgc cagtttgcgt
     8761 ttctggtttc agctctgctt tcagggcgcg gaaacggtca cggatatcgc tcatgttcat
     8821 cgcaccgcca gaatccacca cataaataca ggtcgcaccg tagccttcca tcagctttgc
     8881 ctgctttgcg agattctccg gcgtggtcat atggctcatc atcagaaaac caacggtgtc
     8941 cattccgagc tcgcgggcat actgaatatg ctgggcggaa acatcagctt cggtacagtg
     9001 cgttgccaca cgaaccaccc gcgcgccagc ctgccaggca tttttcagat cgtgaatagt
     9061 gccgattcct ggcagcaaca acgtcgcgat tttggcgtgc ttcaccacat ccgccgctgc
     9121 ttcaatccat tcaaggtcgc tatgtgcgcc gaaaccatag ttaaagctgg aaccctgcaa
     9181 accgtcgcca tgggccactt caatcgaatc cacgcgggca tcgtccagtg ctttggcaat
     9241 ctggcgaacg ttttccagcg aatactgatg acgaatggcg tgcataccgt cacgcaacgt
     9301 gacgtccgag atataaagtt ttttaccgtt catgccgctt ctcctgcctt gcgcgccagt
     9361 gactgggcca ttttttccgc tgtcgccagc gcactggaag tcataatgtc gaggttgccc
     9421 gcataggcag gcagataatg cgctgcgcct tcgacttcca gccagaccgc tgttttcagt
     9481 ccggagaatt gccccacgcc cggtaaattg accggtttat cctgcgggat aacttcaaac
     9541 tgcacgcgct gtttcaggcg ataacccggt acgtaagcct gcaccgcctc agccatttca
     9601 ttgattgagg cttcgatatc atcttgtgaa gcttcgtcgc tcaatacata caccgtgtca
     9661 cgcatcatca acggtggctc tgctgggtta agcacaataa tcgccttccc ttttgccgcg
     9721 ccgcccacca cttcaatggc tcgggaagtg gtttccgtaa attcatcgat attggcacgc
     9781 gtgccaggtc cggcagattt actggcgata gaagcgataa tttcggcgta atgaacacgc
     9841 gccacgcgtg aaactgccgc caccattgga atggtggcct ggccgccgca ggtgaccatg
     9901 ttgacgttca gttgatcgac gttcgcctcg aggttaacca ccggcacgca gtaagggccg
     9961 atggcagcag gcgtcaggtc aattaagcga atatccggtt tcgcttcgcg taaagcggca
    10021 tcgtttttca catgcgcgcc cgcgctggtc gcatcaaata caatgtcgat atcagcaaat
    10081 tcaggcatgt tcatcagccc gatcacccct tcatgggtgg tggcgacgcc catacgtctg
    10141 gcactcgcca gaccgtcgga ctgaggatca atgccgacca tcaccgccat ctccagatgc
    10201 tgaccgtgac gcaaaatttt aatcatcaga tcggtgccaa tgttgccaga accgataatg
    10261 gcgactttac gcttactcat gacagacttc cttttggggc tgcgcttgaa aatgtcgcag
    10321 caactgaacc tatgccttca atatgggctt caaaacgatc gcccgcattc accgccacca
    10381 tcgggcctaa tgccccggta agaatgatat ctccggcgcg cagcggttca cccagactgg
    10441 ccattttgcg tgccagccag acggcggcat taagcggatg tcccaggcat tcgctgccgc
    10501 gcccgctgga aacctcttcg ttattacgcg tcatcttcat ggcgcagttt ttcaggtcta
    10561 accccgccgg acgttgcgcc ggaccgccga tgacatacac cccgcaggaa gcgttatctg
    10621 ccacggtatc gacaaactga atcgaccagt cgcgaatgcg gctccccacc acttccagcg
    10681 ccggaagtac ccattcaatg gcgttataca gttcgtcgaa ggtgatatca gttgcgggta
    10741 aatcgcggtt caacaccagt gcgatctccg cttcaatgcg gggttgcaga acacgggaaa
    10801 aaggaatgat ttcgttatcg ccataacaca tgtcggcaaa taacgtccca aaatccggtt
    10861 gatcaacgcc cagttgttgt tgcactttcg gatgcgtcag gcccacttta cgccctacca
    10921 cgcgacgccc ctgcacaacg tcatattgca catttatgtg ctgaatggcg taggccgctt
    10981 cagcgttatc gataccaatc agatcgcgca gcggcgcaat cgcttcgccc tgctctgcgg
    11041 cgcggcgtaa atccgccgcc agttgctcaa gagtatgctt cgtcatgacc attccttaag
    11101 ggcgtgcgag gaaattcagc accaattgat tgaaagcgtc ggcatgttcc cactgcgccc
    11161 agtgaccaca gtcgcggaag atatgcagtt cagaaccggc aatgccggac agcagacgca
    11221 gacccgcatc catcggcaca aagcggtcgt tgcgccccca gacaatcagg gtttgcgctt
    11281 tgatttccgc cagacgtggg ccaaaatccg ggaactgttt cggattagct tccaggctct
    11341 taacgaagtt ttcctggtga tcgcggcgcg acagcatatt attcaggcgc gcttcaaaca
    11401 gggcgtcggt caaatcgctg gtatcgaaaa cgaagatatc catcatcagc ttcaggtttt
    11461 cgatagtcgg ctgacgataa agctgattca gtcgcttaat accttcggtt ggcatcggcg
    11521 taaacaaact catgccgccc gtaccgccgc ccatcagcac cagtttgccg acgcgctccg
    11581 gccagttaag ggtgaacgcc acagaactat ggccgcccat cgagttgccc agcaggtgga
    11641 ttttggcgat atccagttga tccaccacgc ttttcaggat tcgtgcatta agatccgatc
    11701 gtgaaccact attaacgatc gaatcgcttt tgccccaacc cggacaatcc agcaggatta
    11761 cccgatagcc cgcctctacc agcggatcga tattgcggct gaagttcgcc cagccagtag
    11821 cacccgggcc ggaaccatgc agcaggacaa cggtttcgtc gccttgtccg cagtcattaa
    11881 aatggatgcg cagcgtttta cccgcttctt ctacattcag aaaacggctg gtggcggctt
    11941 cggtttgtgg ctgataactc atcatcttct cctgcatatt cagttctctg ttctggcgct
    12001 taacgagcca aatccggcaa tccactccgg gattgggcgg taataacgcc cttcgctacg
    12061 ccagttgcca aatgtagaaa tagcggcaaa agcggcgacc caggttttga tttcatgtgt
    12121 cgactttccg gcaatggcgg aaagctcttc gttactgacg gcatccagtt cctgtatgcg
    12181 tccctgctcc agcaaagtca tgaactggtt atcccaaatc gggttgagcg gatgcagagt
    12241 tctctgatcc tcaacaaact tctcagcggc gctaatcacc cgttgctgac gcaattcgcg
    12301 ctcactggcg ggtaaatctt tcccgctccc caacagacgg tcgcgcatat gggcatcggc
    12361 tttcgccagt tcgggcaccg gcggctgatg ggaaagccca ccggaaccca ggaacagcac
    12421 gcgtttattg agagtgctgg tgaaacgtcc aatggcttca cccaacatgc gggtacgctg
    12481 gaaaccgggc agcggcgtgg cgacaccgtt gatgaacaca ggcagaactg gcaccttatc
    12541 cagcccaccg agcaggaact ccagcggctg ggcgaacccg tggtccacct gcatacagta
    12601 agaaacggca agatcgatcc cgctcttcat gacagcatgc gcacaggcct ccgccagctc
    12661 cacaggcacg ggcagctctc ctgccgcact gccgaaatca ccaattgccg tcgctccaac
    12721 gcctaaacag aacggtggca tcacgtcata gaaaaagccg ttgtagtgat ctggcgcaaa
    12781 cagcaccacc agttcagggg agaaagccgc aatacgctcg cgggcgctgg caatcacgcc
    12841 attgacctca tcgagtacct cttgcgccgg gtcgacatac cccaccaacg gcgagtggga
    12901 aagacagtga agataagcgt gcatatcagg ctaccttttc gacagaaacg tcggcatcag
    12961 ggcgggtcag cgtcatcacc gacgccagtt tattcagcgt attgcccagg gtttgcggaa
    13021 tggcggtggc ggcaacaaag cgatccgggc gcatcaccac cagcgaagca ttgtgttgcg
    13081 cgaaccagct acgcagacga ccttgcgtat cgcccacgcg tagcacgccg tcgtggttat
    13141 cctgtgcggt atgaatttgc acttccggca ccacctgaat gaagcgtgtg cccaacgcgc
    13201 gccactgctg gatttgctcg tcgctcatcc cccacagtgg attgcatccc cagccaatta
    13261 ccgcgaagtt cgcgccaatc gcgttatcga gcagcgtcac gtcgccgttt tccagcgtga
    13321 ctttcggctg aataaacatc ttgccgaccg gagagtgctt cgcttcgccc tcacgtacca
    13381 gcgcaccgcc gtaatattgc ggcatcggct tgaagcgcat ttcgaggaag tagcgtttta
    13441 ctggcggcag ataattcagt agccaggaaa cgccgtcacg taacgtaccc tgccagcgtt
    13501 tcggcggagc cagcacgttg cccgccgtca cggacaggtc aatcatcgct ttggcgtgat
    13561 cgcgacgttc ttgttgatag gtatcgagca gcacatcgcg ggctttcccc tggataacca
    13621 acgccagttt ccaggcgagg ttaaaggcgt cgcgcatacc actgttatag ccctgcccct
    13681 gccacaccgg catgatgtgc gcggcatcgc ccgccagcag tacgcgatca atacggaaac
    13741 gttgcgccag tcgcgcgtta tgggtgtaga cacgctggcg aatcaattca acattgtccg
    13801 gattaggcag cactttgctt aacagcttgc gcatattttg cggctcacgc agctgctctt
    13861 cggtttctcc cggcatcacc ataaattcaa agcgacgtac cgcatgaggc agcgcggcag
    13921 aaacatacgg gcgcaccgga tcgcaacaca aatagatatg cggcgtactt aacggatcgt
    13981 tggcgatatc taccacaatc cactgatttg gcgcagtttt accttcaaac ggcacattca
    14041 gagtgcgacg gacaaaactt gctccaccgt cacaggctac cagccactgg gctttgacta
    14101 tttcccgctg cccttctgcc gttttcaggt gcaaggtcac ttcgtcattt tgctggctga
    14161 aggcctccag ctcgcgggaa aacaagcagc gcacattagg aaaacgcgac accccttcca
    14221 gcatcaccgc atcgacctgc ggctgaataa aggcgttacg gcgcggccag ccaaattcat
    14281 cggtcattgg ctgaatatca gcaaaacagc ggcctttcgg ggtgagaaaa cgcatcgcgt
    14341 gccacggcgt agtgtgcggc agaacattct cgaccaggcc aaccgactgc atggtgcgca
    14401 gcgcctcgtc atcaatacca atcgcacgcg ggtagtcgat caacttatcg agtttctcca
    14461 ccaccagcac gtcaatgccc atctgaccga gatagttcgc catcatcagc ccgaccggac
    14521 cggcacctgc gatcgccacc tgaacgctat ggttaacagc aggctggatg tcagggtgtt
    14581 gtattgccat ttcagtacct cacgactcgg acaaaatgcc gttgcgcgca cagtacggcg
    14641 caacttattt tgttaaaaac atgtaaatga ttttttattg tgcgctcagt ataggaaggg
    14701 tgttttcggc tacaatcaaa acatgcccga atgtgcacca ggtgcacaac gttgttttaa
    14761 ctatagaaat gtcaattaat atgcagaaca atgagcagac ggaatacaaa accgtgcgcg
    14821 gcttaacccg cggtctaatg ttattaaata tgttaaataa acttgatggc ggtgccagcg
    14881 tcgggctgct ggcggaactc agcggcctgc atcgcaccac tgtgcggcga ctgctggaga
    14941 cgctgcagga agagggatat gtccgccgta gcccctccga tgatagtttt cgactgacca
    15001 tcaaagtgcg gcaattaagc gaaggatttc gtgacgaaca gtggatttct gcactggcgg
    15061 ccccactgct gggcgatctg ttgcgcgaag tggtatggcc gacagatgtg tccacgctgg
    15121 atgttgatgc aatggtggta cgcgaaacca ctcaccgttt cagccgctta tcctttcacc
    15181 gggcaatggt cgggcgacgt ttgccgcttc tgaaaaccgc ctcgggcctg acctggctgg
    15241 ccttttgccc ggaacaagac cgcaaggaat taatcgaaat gttagcctcc cgccccggtg
    15301 atgactatca actggcacgg gaaccgttaa agctggaagc cattctggcg cgcgcgcgca
    15361 aagagggtta cggacagaac taccgcggct gggatcagga ggagaagatc gcctctatcg
    15421 ccgtaccgct gcgcagtgaa caacgggtga ttggctgtct gaatctggtg tatatggcga
    15481 gcgcaatgac cattgaacag gcagcggaaa agcatcttcc ggcgctacaa cgggtagcaa
    15541 aacagatcga agaaggggtt gaatcgcagg ctattctggt ggccggacgg cgaagcggcg
    15601 tgcatttacg ttgacaccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgt
    15661 tcggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgctata cgatgtcgca
    15721 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt
    15781 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac
    15841 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
    15901 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc tgatcaactg
    15961 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg
    16021 gtgcacaatc ttctcgcgca acgcgtcagt gggctgctca ttaactatcc gctggatgac
    16081 caggatgcca ctgctgtgga agcagcctgc gctaatgttc cggcgttatt tcttgatgtc
    16141 tctgaccaga ctcccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc
    16201 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt
    16261 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt
    16321 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg
    16381 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg
    16441 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta
    16501 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa
    16561 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
    16621 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg
    16681 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    16741 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    16801 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtgaaat
    16861 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg
    16921 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacacaactt aatcgccttg
    16981 cggcacatcc ccctttcgcc agctggcgta atagcgaaga agcccgcacc gatcgccctt
    17041 cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag
    17101 cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct
    17161 caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtgacc tatcccatta
    17221 cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta
    17281 atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact
    17341 cggcgtttca tctgtggtgc aacggtcgct gggtcggtta cggtcaggac agtcgtttgc
    17401 cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg
    17461 tgctgcgctg gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca
    17521 ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg
    17581 ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg
    17641 gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg
    17701 ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtagt tatgccgatc
    17761 gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc
    17821 tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct
    17881 gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc
    17941 cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg
    18001 atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc
    18061 gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt
    18121 atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg
    18181 atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc
    18241 gtaatcaccc gagtgtgatc atctggtcgc tggggaatga gtcaggccac ggcgctaatc
    18301 acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag
    18361 gcggcggagc cgacaccttc gcaaccgata ttatttgccc gatgtacgcg cgcgtggatg
    18421 aagaccaacc cttcccggcg gtgccgaaat ggtccatcaa aaaatggctt tcgctgcctg
    18481 gagaactgcg cccactgatc ctttgcgaat atgcccacgc aatgggtaac agtcttggcg
    18541 gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc ggcttcgtct
    18601 gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg tggttggctt
    18661 acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgcatgaac ggtctggtct
    18721 ttgccgaccg cacgccgcat ccggcgctga cggaagcaaa ataccagcag cagtttttcc
    18781 agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg
    18841 ataacgagct cctgcactgg atggtggcgc tggatggcaa gccgctggca agcggtgaag
    18901 tgcctctgga tgtcgctcca caaggtaaac agttgattga actgcctgga ctaccgcagc
    18961 cggagagcgc cggacaactc tggcttacgg tacacgtagt gcaaccgaac gcgaccgcat
    19021 ggtcagaagc cggacacatc agcgcctggc agcagtggcg tctggcggaa aacctcagcg
    19081 tgacactccc cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt
    19141 gcatcgagct gggtaataag cgttggcaat ttaaccgtca gtcaggcctt ctttcacaga
    19201 tgtggattgg cgatgaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac
    19261 cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg
    19321 aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgctcggcag
    19381 atacacttgc cgacgcggtg ctgattacga ccgctcacgc gtggcagcat cagggaaaaa
    19441 ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg
    19501 ttgatgttga agtagcgagc gatacaccgc atccggcacg gattggcctg acctgccagc
    19561 tggcgcaggt cgcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg
    19621 accgccttac tgcggcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc
    19681 cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc
    19741 cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga
    19801 tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg
    19861 gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtgtcg gcggaattcc
    19921 agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaaaaataa taataaccgg
    19981 gcaggccatg tctgcccgta tttcgcgtaa ggaaatccat tatgtactat ttaaaaaaca
    20041 caaacttttg gatgttcggt ttattctttt tcttttactt ttttatcatg ggagcctact
    20101 tcccgttttt cccgatttgg ctacatgaca tcaaccatat cagcaaaagt gatacgggta
    20161 ttatttttgc cgctatttct ctgttctcgc tattattcca accgctgttt ggtctgcttt
    20221 ctgacaaact cgggctgcgc aaatacctgc tgtggattat taccggcatg ttagtgatgt
    20281 ttgcgccgtt ctttattttt atcttcgggc cactgttaca atacaacatt ttagtaggat
    20341 cgattgttgg tggtatttat ctaggctttt gttttaacgc cggtgcgcca gcagtagagg
    20401 catttattga gaaagtcagc cgtcgcagta atttcgaatt tggtcgcgcg cggatgtttg
    20461 gctgtgttgg ctgggcgttg tgtgcctcga ttgtcggcat catgttcacc atcaataatc
    20521 agtttgtttt ctggctgggt tctggctgtg cactcatcct cgccgtttta ctctttttcg
    20581 ccaaaacgga tgcgccctct tccgccacgg ttgccaatgc ggtaggtgcc aaccattcgg
    20641 catttagcct taagctggca ctggaactgt tcagacagcc aaaactgtgg tttttgtcac
    20701 tgtatgttat tggcgtttcc tgcacctacg atgtttttga ccaacagttt gctaatttct
    20761 ttacttcgtt ctttgctacc ggtgaacagg gtacgcgggt atttggctac gtaacgacaa
    20821 tgggcgaatt acttaacgcc tcgattatgt tctttgcgcc actgatcatt aatcgcatcg
    20881 gtgggaaaaa cgccctgctg ctggctggca ctattatgtc tgtacgtatt attggctcat
    20941 cgttcgccac ctcagcgctg gaagtggtta ttctgaaaac gctgcatatg tttgaagtac
    21001 cgttcctgct ggtgggctgc tttaaatata ttaccagcca gtttgaagtg cgtttttcag
    21061 cgacgattta tctggtctgt ttctgcttct ttaagcaact ggcgatgatt tttatgtctg
    21121 tactggcggg caatatgtat gaaagcatcg gtttccaggg cgcttatctg gtgctgggtc
    21181 tggtggcgct gggcttcacc ttaatttccg tgttcacgct tagcggcccc ggcccgcttt
    21241 ccctgctgcg tcgtcaggtg aatgaagtcg cttaagcaat taatgtcgga tgcgccactg
    21301 ggcacatccg acaattattt atagctccat catctggcga aacaagcgtc gcatcatacc
    21361 tctttgaccc acagctgcgg aaaacgtgct ggtgcaaaac gcagggttat gatcatcagc
    21421 ccaacgacgc acagcgcatg aaacgcccag tccatcaggt aattgccgct gatactacgc
    21481 agcacgccag aaaaccacgg ggcaagcccg gcgatgataa aaccgatccc ctgcataaac
    21541 gccaccagct tgccagcaat agccggttgc acagagtgat cgagcgccag cagcaaacag
    21601 agcggaaacg cgccgcccag acctaaccca cacaccatcg cccacgatac cggcaattgc
    21661 agcggcaacc agataaagcc gcagaacccc accagttgta acaccagcgc cagcattaac
    21721 agtttgcgcc gatcctgatg gcgagccata gcaggcatca gcaaagctcc tgcggcttgc
    21781 ccaagcgtca tcaatgccag taaggaaccg ctgtactgcg cgctggcacc aatctcaata
    21841 tagaaagcgg gtaaccaggc aatcaggctg gcgtaaccgc cgttaatcag accgaagtaa
    21901 acacccagcg tccacgcgcg gggagtgaat accacgcgaa ccggagtggt tgttgtcttg
    21961 tgggaagagg cgacctcgcg ggcgctttgc caccaccagg caaagagcgc aacaacggca
    22021 ggcagcgccc accaggcgag tgtttgatac caggtttcgc tatgttgaac taaccaggga
    22081 gttatggcgg caccaagtcc accgccgccc atcagagccg cagaccacag ccccatcacc
    22141 agtggcgtgc gttgctgaaa ccgccgttta atcactgaag gcatcaccgc ctgaatgata
    22201 ccgatcccca ccccaccaag cagtgcgctg ctaagcagca gcacactttg ggggtaaagc
    22261 tcacgcatca atgcaccgac ggcaatcagc aacagactga tggcgacact gcgacgttcg
    22321 ctgacatgct gatgaagcca gcttccggcc agcgccagcc cgcccatggt gaccaccgga
    22381 agagcggtca acagggcagc cacgctaaag ctcattccgc tcgcctggcg caattgcggt
    22441 agcagtggcc cgacggaggt gagcagtggt cgcatattaa gaccaatcag caccagaacc
    22501 agcagcatcg tgaacgtctt ttgatgattg cgcatggctg tcagaacggc ttggtcggca
    22561 gatatttacc atctaaggta atgaccgcac gttcgccacc ttccgggtcc gccactttct
    22621 taacgtcgag tttgaagtta atcgcgctga taatgccatc gccaaacttc tcatgaacca
    22681 acgctttcag ggttgtgccg tacacctgca acatttcata gaaacgatac atcgttgggt
    22741 cggttggaat acggtcatca atgcagccac gcagtggaat catctgcaac agtagaatgg
    22801 cgtcttcgtc gagatccagc ttcgccccga ccaggcgggc ggcgtcggca ggaagcgctt
    22861 gctgccccag caaagccgcg gttacgaagg cttctgccag accggtaccg tcggcaatct
    22921 cggcaaatga gagatctttt ttagctttgc tgagcaaaat ggcatcggca agatcaagac
    22981 gaatattgcg gttaatttgt gactgaatca tggtggatct cctgatggtt taaaaataag
    23041 gttacgctgc ggtcggttgg cgtagcggta tggcacaaac gcgaggatta gcggccagtg
    23101 gcacaaactg gcgggttgcg ccgtcaaaag ctgcgatgct gccgctttca atgtcgtaga
    23161 cccagccgtg cagggcgatc cgcccctctt cgagcgccag gcgcaccgat ggatgagttt
    23221 gcaaattagc caactgagca atgacgtttt cacgtaccat cgccgcagct tttgacggta
    23281 aatcggaatg cgggcgcgcc tcattaacga cgcgggctga atcggcataa cgcagccagt
    23341 gggagacggc aggcatatgg tccatgcact gacagctggc aatggcggtc atcgcgccac
    23401 agttggaatg accacaaatc acaatgtcag atacccgaag cgcagcgacg gcatactcca
    23461 ccgaagcaga aacgccaccg ggttccggcc cgtaggaagg gacgatattg cccgcgttgc
    23521 gaataacgaa cagatcgcca ggctcacgtt gcgtcaccag ctcagggacc agacggctgt
    23581 cggagcagga gataaaaagt gtgcgcgggc tttgctgtgt cgccagctgt ttaaacaagg
    23641 cttcccgctt cggaaatgcc tcgcgctgga atttaaggaa tccatcaata atctctttca
    23701 ccgttaacct ctgtctgtct ctggaatgag aggcagacta ctcgccgatt gtcataaggt
    23761 aaaagtctca tttatgatga gttctattgg atttacttat aggttggcga atgctctctc
    23821 gacatatcaa ttattttctt gccgtggctg aacatggcag cttcacccgt gccgccagtg
    23881 cgttgcacgt ctctcagcct gcgctttccc agcagattcg ccagttagag gagagtttag
    23941 gcgtgccgct gtttgaccgt agcgggcgaa cgattcgtct caccgatgca ggagaagtct
    24001 ggcgacagta cgccagccgg gcgttacagg aactgggagc gggtaaacgg gcgattcatg
    24061 atgttgccga tctgacgcga ggatcgctgc gtatcgccgt cacccccacc tttacaagct
    24121 actttatcgg ccctttaatg gcggatttct atgcgcgcta ccccggcatc acgctccagc
    24181 tacaggaaat gtcgcaggag aaaatcgaag atctgctttg ccgcgacgag ctggacgttg
    24241 ggattgcctt cgcgcctgtg cattcgccgg agctggaggc aattccttta ctgacagaaa
    24301 gtttagcgtt agtcgtggcg caacatcatc cgctggctgc ctgtgaacag gtggcgttga
    24361 gtcgcttgca tgatgaaaaa ctggtcctgc tcagcgcgga atttgccacc agagagcaaa
    24421 ttgaccacta ctgcgagaaa gcggggctac atccacaggt ggtcattgag gcgaactcaa
    24481 ttagcgcggt tctggagctg attcgccgca cttccctttc cacattgtta ccagcagcga
    24541 ttgccacaca acatgacggg cttaaagcga tttctcttgc cccgccacta ctggagagaa
    24601 cggcggtttt gttgcggcgg aaaaatagct ggcagacagc cgccgcgaag gcatttttgc
    24661 acatggcgtt ggaggaatgt gcggatgttg gcgagaatga atcacggtag ccactgacac
    24721 aggtcagtgg ctcttcaaca cagaggaaat taccgtttgt aatcgatggc ttctggctgc
    24781 tccagatata cggtggtttg tgccggttgt gtactggcaa tcaccttgcc gccacgtacc
    24841 gaataacgta ccggaacctg acggcgcagc gcatcaaatc cattttcagc cggcaggata
    24901 atcaggtttg cgctgtttcc ggcggcaatg ccgtaatcct gcaaattcaa tgttctggca
    24961 ctgtggtggg tgattaaatt caggccatcg ttaatctgcc cgtagcccat caactggcaa
    25021 acatgcagcc ccatatgcag cacttgcagc atattcgccg ttcccagcgg ataccacgga
    25081 tcgaagacat catcgtgacc aaagcagacg ttaatgccgg actccagcat ctctttaacg
    25141 cgcgtgatgc cgcgacgttt tggatacgta tcgaaacgtc cttgcagatg aatattgacc
    25201 agcgggttgg cgacaaagtt aataccggac attttcagca agcggaacag gcgtgaggta
    25261 tacgccccgt tataggagtg cattgccgtg gtgtggctgg cggtgactcg cgcgcccatg
    25321 ccttcacggt gcgccagggc agcaacggtt tcgacaaagc gcgactgctc gtcatcgatc
    25381 tcatcacagt gaacgtcgat gagacggtcg tatttttgcg ccagggcgaa ggttttatgc
    25441 agcgactcca cgccgtattc acgggtaaat tcaaaatgcg gaatcgcccc cactacatct
    25501 gcccctaagc gtaacgcctc ttccagcaac gcttcaccgt tgggatacga caaaatccct
    25561 tcctgaggga aggcgacaat ttgcagatca atccacggcg cgacttcctg cttcacttcc
    25621 agcattgctt tcagcgcagt tagcgttgca tccgaaacat cgacatgggt acgcacatgc
    25681 tgaatgccgt tggcaatctg ccatttcagc gtttgccagg cgcgttgttt cacatcgtca
    25741 tgggttaata acgctttgcg ctcggcccag cgttcaatgc cttcaaacag cgtgccggac
    25801 tgattccagt tcggttgtcc ggcggtttgc gtggtgtcca ggtgaatatg cggctccaca
    25861 aacggcggta taactaaacc ttgttcggca tccaggctgt tttcagttat gggcatcacg
    25921 ccggattgcg catcaatggc gctgattttt ccgtcctgca gatgaatctg ccacagcacc
    25981 tctttgcctg gtaaccgggc gttaataatt gtttgtaaag cgttattcga cactgttaac
    26041 ctccacatgc gtcattgctg ctgtcgtttt acgattcaaa atcgggttaa ggatcagata
    26101 gctcagcgcg ccacctaata ccgcgttgac cggaacaatt cccggtaacc agtggcctgc
    26161 ggcaatcccc agcgcgaccg ccagaatcgc cacccaattg acactcatca tacgcgtggt
    26221 cgcaaagtgc tcatagcggc gacggttcat cagatagtcg gcgatgatca cgccccctac
    26281 tggaggaata gctgccgaaa ggaaggtcaa ccagccgaca aaattgttat acagccataa
    26341 tgcgcagacc gtaccgataa taccgttgat taccgaaagg gttttgctcg acataccggt
    26401 aatgttggcg aaacctaaac ccgacgcata gagtgcgtta tcgttggtgg tccagatatt
    26461 cagccccagc accacaatcg caggcagcag caggccctga gcaatcatca catcagagat
    26521 atccgccatg cccagtgccg cagcgcccgc tgcaccgaaa ataaacatca acgagttgcc
    26581 gaggaaaaag gccaccatcg ccaccagcac cgccagtttg gcattgcgac caaaccggac
    26641 aaagtcagcg gtgagcgtac ccgcactgat aaatgacccc acaaccagcg ccagcgcgac
    26701 attgaaatct aacggttgtg cgggaacgac cgcttttaat gcgtccaggc cgcccatgcc
    26761 gttaacagcc agccacacgg aataaccgcc caggcaggcg atagccggaa ccgcaatcac
    26821 cgaaagaacc gtcagcgccg aaatgccaaa aaagacggtg acggtcatca gtaaaccgga
    26881 aacggcaatc agcaaattaa tatccagccc ggttgcctta cccaccggaa tggcaaacat
    26941 cgccacaccg acgccaaacc agccaacctg agttccgccc agtagcagtg aaggcagcca
    27001 tgagccttta acaccaaacg agaagcgagc aagaagatga gtggtcaggc cggtttttgc
    27061 gccaatgtaa ccgagaaatg aagtgtaaat accgaggaga agattaccga tgagaactgc
    27121 gaggaagaaa tcatgatagc taagaccggt tccgagagtg ccgccggtcc acatactggc
    27181 ggaaaagaag gttaatccca gcatgacgaa cgtcaatgcc aatacccctt tccgcgccga
    27241 ctgcgggact ggcccctggc taaagttgtt atcttgcgac acgaaattcc ccctttttgc
    27301 tgtttgaaac ccaaaaaatc cgccgtattc tattcatctg aagataaaaa gcaatcgttt
    27361 tcgtggggaa atatattttt tatatgaatg agtgcaaaaa ggtgggggaa aaattatcgg
    27421 tgtaaaaaca gtgggtagtg ggtagatact gataagacgc gtcagcgtcg catcaggcat
    27481 cggcgcgggg tgtcggatgc gacgcgagcg ccttatccga cctacggttc aggcctgtag
    27541 gcataataag acgcggtaag cgtcgcatca ggcatcggcg catggtgtcg gatgcgccac
    27601 gcgacgcatc cgacaatacc ttactcttcc atcgcctggc ggatctgatc caatgacgca
    27661 ggatcatcaa tggtcgtcag atccccagga tcgcgtcctt cgcaaattgc ctggatcgtg
    27721 cggcgcagca tttttccgga tcgcgttttc ggcaattgcg agacaaacca gacgtgcgcc
    27781 gggcgaccaa agttgccaat ctggctgtcc accagcgcca taatcgcctt ctcttgcgaa
    27841 tgcgccacat cacgatcttc cagactatcg ctctctttcg gaatgacaaa cgccaccgcc
    27901 acctgccctt tcagcgcatc tttcacccca accaccgcca cttcggcaac gcccggatgg
    27961 ctggagatac tctcttcaat ctcgcgcgtg cccagacgat gcccggcaac gttaattaca
    28021 tcgtcagtgc gcccgagaat aaagtgataa ccgtcagcgt cacggatgcc ccagtcaaag
    28081 gtggcgtaca ccgggcggga aaacagcgac cagtaagtct tcacaaagcg gtcgtcgtcg
    28141 ccccagatgg tctgaataca gcccggcggc agcggccctt ccaccaccag catccctttc
    28201 tcgttgacgc cacacggttc gccggtgact tcattaagca actgcacgtt atagccatac
    28261 atcggcacgc cggggcttcc cagacgcgtc ggcctgtcgt ccagaccgcg agcaatcgcc
    28321 ataatcggcc agccggattc ggtctgccag tagttgtcga tgaccggcac atccagcgta
    28381 ttgctcaccc aactggcggt cggctcgtcc agcggttctc cagccagata gagcacttcc
    28441 agcgacgaga gatcgtgttt gcgaatttca gcggtaggga attttttcag cacgcgaatg
    28501 gcggtcggcg ctgagaacat ccggctaacc tgatatttct cgacgatttt ccaccacacg
    28561 ccgcagtccg gccaggtcgg caatccttcg taaacgatag tcgccatccc cgccagcagc
    28621 ggcgcgtaaa cgatatacga atgccccacc acccagccga tatccgatgc gcaaaagaac
    28681 acgctgcccg ctttgccgcc aaaaatggtg tccatcgagg tcgccagcgc caccgcatat
    28741 ccgccgacgt cacgctgcac gcctttaggt ttgccggtcg tgccggaagt gtagagaatg
    28801 caggaggttt cgttggattc cagccacgcc accggtaccc gcgcgccgat gtgttgatgg
    28861 cgcaacgacg cgaaatcgac atcccgcccg ctgacgcgcg ccattttcgc cagcccgcga
    28921 tccaccagca aaacatggcg tggctggtgc tgcgcctgac ttatcgcatc gtcgagcaat
    28981 tttttatagg gaatgatttt gccaccgcgc gctccggcat cagccgagac aatcagcacc
    29041 ggtttagcgt catcaattcg cgccgccacg ctgtgcgagg caaaaccacc aaataccacc
    29101 gaatgaatag caccaatgcg cgcgcaggcc agcagagtaa tatgcgcttc ggcaatcatc
    29161 ggcatataca ccagcacccg atcgccgcgc tgcacgccca gtgagcgcaa cattgaggcc
    29221 accgcgttca cttcgtcatg cagctggcga aaggtaaagg tgcgctcttc ttctgtttcc
    29281 gaagagacgg caatcaatgc cagtgcctct ggctgtttct ccagccagcg gtcgatagcg
    29341 ttgtggcaca aattggttcg gccttcacaa aaccaacggg caaacggcgg attgctgtgg
    29401 tcgagcgttt gcgtaaaggg cgtctgccag tcaatacgcc gggcctgctc ggcccagaac
    29461 tgctccggtt cgttaatcga acgctgataa aattcgctaa aagacataat gctctcctgt
    29521 tgaacttacg ccttaccgca gtttacttaa atgacgtaca ggtcgagata ctcattgacc
    29581 ggcatctgtt ccaggcgagt tctgtcgaga gaaacctcca gaatgcgctg ctgttggcga
    29641 gtcgggaact ggcgcgcgag attgattttg aatttatcga ccagtttcgg aataccatcc
    29701 tgacggcggc gagcatgacc aatggggtac tccaccacca cttcttcaaa tcgtgtgccg
    29761 tcggtgaact caagggtaat ggcattggcg atggcgcgtt tttccgggtc gtggtagtca
    29821 gcggtaaatg ccggatcttc aaagcaattg atcttctcgc gcagggcgtc gatgcgttta
    29881 tcttgcgcaa cgttgtcctc gtaatctgcc gccgttaagc gcccgaataa cagcgggatc
    29941 gccaccatgt actgaatgca gtggtcgcgg tcagccgggt tattgagcgg cccctttttg
    30001 tcgatgatgc gaatacaggc ttcgtgggtg cgaatggtca ctttttcgat atccgccgcc
    30061 gtcttgcctg ctgcctgcat ctgttcatag agcgtcatcg ccgcttcaac tgccgtctgg
    30121 gagtggaact ccgccgggaa ggagattttg aacagcacat tctccatgac gtaagaaccg
    30181 tacggacgct ggaagcggaa cgattcacct ttaaaggaga cgtcgtagaa gccccatacc
    30241 ggcgcggtca gggctgacgg gtaacccatt tcgcccgttt tcgccatcag tgccagacgt
    30301 accgcgcggg aagtggcatc gcccgccgcc caggatttac gcgtgccggt gttcggcgca
    30361 tggcgatagg tgcgcagcga ctgaccgtcc acccacgcca gcgaaacggc gttgagaatt
    30421 tcctcgcggg tcaggccgag catttcggcg accacggcgg tggaagccac tttcactaac
    30481 agaacgtggt cgaggccgac gcggttaaag gagttttcca gcgcgatgca gccctgaatt
    30541 tcatgggctt tgatcattgc ggtcagcacc tgtttcatgg tcaacggcgc tttgccgctg
    30601 gcgacagcgt tgcgcgaaag ccagtctgcc gttgccagaa tgccgccgag gttgtcggaa
    30661 ggatgccccc actccgccgc cagccaggta tcgttgaaat cgagccagcg gatcatcgcg
    30721 ccaatgttaa atgccgcctg gacagggtcg agctgaaact gagttccggg gacgcgcacg
    30781 ccgttgggta cgacggtgcc aggaacaatt ggccccagca gttttttaca ggccgggtat
    30841 tcgagagctt ccagaccgca gccgagcgtg tcgagcaggc agtaatgtgc ggtgtcgtag
    30901 gccactttag agctgatttc gtagttcatc acgtaatcga cgatatcaac gatttcacga
    30961 tcaaattccg ggcggatgtt gttgatttga gctgacattg ggtatgtttc cttattgtta
    31021 ttcgtagagg tttactggcg cttatccagc gcgacaaact ggcggtcttc cggtccaaca
    31081 taattggcgg aaggacggat aattttgttg tcctgacgtt gttcaatgat gtgcgccgcc
    31141 cagccggtga cacgggcgat gacaaacagt ggtgtgaaca tctcggtggg aacgcccatc
    31201 atgttgtagg aaacagcaga gaaccagtcg agattgggga acatcttttt gctctcccac
    31261 atcaccgttt ccaggcgatt ggcgatgttg tacatcttca acgagccgcc ttcctgcgag
    31321 agctgcttcg ccacacgttt gatcacctgg tggcgcgggt cggcgatggt ataaaccgga
    31381 tgaccaaaac caatgaccac ttctttgttt tccacccgct tgcggatatc ggcttcggct
    31441 tcgtccggcg tttcgtagcg ttgctggatc tccagcgaca cttcattcgc tccgccgtgt
    31501 ttcggcccgc gcagtgcgcc aatcgcgcca ataatcgcgg aatacatatc agagccagtg
    31561 cccgcaatca cccgactggt aaaggtggag gcgttaaact cgtgttcggc gtacagcact
    31621 agcgaaatat gcatcgcctt ttcccagctt tgcgatggct tttcgccgtg cagcagatgc
    31681 aggaagtgac caccgatgga gtcgtcatcg gtttccggct ggatgcgttc gccgttgtga
    31741 ctgtagtgat accaataaag gagaatcgag ctaagtgatg ccagcagttt gtcggcaata
    31801 tcccgcgcgc cagagacagt gtgcccctct ttttctggca gtgtgcagcc gagcgcggaa
    31861 acgccggtgc gcatgacatc catcgggtac gacgccgccg gtaaggcttc cagcacggta
    31921 cgcacgttag ccggtaaacc gcgcagggct ttcagtttcg ttttgtaagc ggcgagttcg
    31981 tcacgggtcg gcagtttgcc gtggatcagc aggtgcgcca cttcttcaaa ttcgcaatgt
    32041 tccgccagat caagaatatc gtagccacgg taatgcaggt cattgccact tttacccacg
    32101 gtgcagagcg ccgtattgcc cgccggaacg ccagaaagtg ccacagattt tttcggttta
    32161 atgacatggg tactgttttg caggatcgtt gtgtcgctca tgttgtcctc gtcatttgta
    32221 gggtcgagat atttttcgtt gtgttttatt tcacctggct acgggcaaac aggtcgtcga
    32281 gcttcgcttc gtactggtag tagttgatgc tttcgtacag ctcgttgcgg gtttgcatgg
    32341 tatcgatgac gcttttctgc gtgccttcct gacgcaggac gttgtagaca tgctcagcgg
    32401 cgcggttcat ggcgcggaac gctgaaagcg ggtacagcgc cattgcgaca tgggcgctgc
    32461 gtaattcgtc gatggtaaac agcggcgtgg caccaaattc ggtaatgttg gcgaggattg
    32521 gcacctgcac cgcatcggca aactggcggt acatggcgag ttcggtaatc gcctccggga
    32581 acaacatctc ggcacccgct tcaacatagg cctgcgcacg ctcgatcgcc gcatccagcc
    32641 cctctaccgc cagagcatcg gtgcgcgcca tgatcacaaa atcaggatcg gttttcgcat
    32701 ccaccgccgc gcggatccga tccaccatct cttctttcga gacgatcgct ttattcggac
    32761 gatgaccgca gcgtttcgca ccaacctgat cttcaatatg caatcctgcc gcaccggctt
    32821 taatcatcga tttcacggtg cgcgccacgt taaaggccga agaaccaaaa ccgatatccg
    32881 catccaccag cagcggcagc gaacaaacgt cggtgatacg gcgaatatcg gtcagcacgt
    32941 catcaagagt agaaataccg agatcgggca gccccagcga acctgccgcc acgccaccgc
    33001 cagaaagata aattgcctga tatccggcac gctgcgccaa cagcgcatga ttagcgttga
    33061 tggtgccaac aatctgcaat ggattttctt tagtcagtgc agcgcgaaac gctttacctg
    33121 gagagtgtag agacatagcc catcctttgt tatcaacttg ttatttgcgt tgataaagac
    33181 aaagcaaggg gtgtgccaca attgccttaa taaaattaaa tgtttaattt caatttgtta
    33241 gagataaagt catgaactaa tccgcaaata tgcgtttcag ttaacgtttc aggcaatgtt
    33301 tcaccgcgtt tcattgcaac aattatgaaa caagactaaa cccaatattc ggtttcttaa
    33361 ctttgcggtg cgctatggca catccaccac ggcttaatga cgacaaaccg gttatctgga
    33421 cggtatctgt aacgcgcctg ttcgagctgt ttcgcgatat cagcctcgag tttgatcacc
    33481 tggcgaacat tacccctatc cagcttggct ttgaaaaagc ggtgacctac atccacaaga
    33541 aactggcaaa cgaacgctgt gacgccatca tcgcggctgg atctaacggc gcgtacctga
    33601 aaagccgcct gtcagtgcca gttattttga ttaaaccgag cggctacgat gtgttacagg
    33661 cactggcaaa agccggaaaa ctcacctctt ctatcggcgt tgtcacttat caggaaacta
    33721 ttccggcact ggtggcgttt caaaaaacct ttaatttgcg cctcgatcaa cgtagctaca
    33781 ttaccgaaga agacgcacgc gggcagatta acgagctaaa agctaacggc accgaagcgg
    33841 tggtcggcgc ggggctgatt accgatctgg cagaagaagc cggaatgacc ggaattttta
    33901 tctattccgc cgccaccgtg cgccaggcgt tcagcgatgc gctggatatg acgcgcatgt
    33961 cgttacgcca taacactcac gatgccactc gcaacgccct gcgtactcgt tacgtgctgg
    34021 gcgatatgct cggtcaatca ccacagatgg aacaggtgcg gcagactatt ttgctgtatg
    34081 cccgctccag tgcggcggtg ttgattgagg gggaaacggg gacgggcaaa gaactggcag
    34141 cccaggcaat tcatcgggaa tactttgccc gccacgatgc gcgacagggc aaaaagtcgc
    34201 atccgtttgt tgccgtcaac tgcggggcga ttgccgaatc gctgctggaa gcggagctgt
    34261 ttggctatga ggaaggggcg tttaccggct cgcgacgcgg aggtcgcgcc gggctgtttg
    34321 aaatcgccca cggtggcacg ctgtttttgg atgagattgg cgaaatgccg ctacctttgc
    34381 agacccggct gctgcgggtg ctggaagaaa aagaggttac ccgcgtcggc gggcatcagc
    34441 ctgttccggt ggatgtgcgg gtcattagcg ccactcactg caagctggaa gaagatatgc
    34501 ggcaagggca gtttcgccgt gacctgtttt atcggctgag cattttacgc ctgcaactgc
    34561 caccactgcg tgagcgggtg gcggatattc tgccgctggc ggaaagcttt ttgaaagtgt
    34621 ctctggcggc gctctccgcc ccgttttctg ccgcattacg cctgggatta caggcaagcg
    34681 aaaccgtgct ggtgcactac gactggccgg gcaatattcg tgaactgcgc aatatgatgg
    34741 agcgactggc gctattttta agtgtggaac cgacgccgga tttaacgccg caatttttgc
    34801 agctgctact gccggaactg gcgcgcgagt cggcgaaaac tcccgctcca cgcttactga
    34861 caccacaaca ggcactggag aaatttaatg gcgataaaac agcagcggcg aattatttag
    34921 gcatcagccg gacgacgttc tggcggcggc tgaaaagctg aactgccgaa tgcagcgcga
    34981 acgctttatc cggcataaat ttcaatagat gatcaccgat aaacttacgc atcgggcatg
    35041 tttacatagg gttcagaatt acttcttctt ataaatattt gccgtgccgt ggatcttatt
    35101 gtcagtttga ccggaggtca gtaccaacac atcagcccct ttttcatccg cttttttgat
    35161 caaatcttct ttcgcgtctg cagtcgacat ttcattgcta gttgaaatat cacctatttt
    35221 ttcatactgc gattcaactt tttcaaattc cgctttggtc atcaattcag cggcatagac
    35281 attggtaacg gctaacgcta acgcaccgac taacatttta gagattattt tcatattacg
    35341 gctccagagt tgttatcaca ctatcatcca cccacattat tgggttgatt aataattagt
    35401 tcagtcaggg cataaacgcc agtaaccgga aataagacac tttgtgatct ctctcgcacc
    35461 cgattatttt tggtagaaat atgatgaagt tacttcactt atttatggat gaaatcacta
    35521 tggatccttt gcacgccgtt tacctgaccg taggactgtt cgtgattact ttttttaatc
    35581 cgggagccaa tctctttgtg gtagtacaaa ccagcctggc ttccggtcga cgcgcagggg
    35641 tgctgaccgg gctgggcgtg gcgctgggcg atgcatttta ttccgggttg ggtttgtttg
    35701 gtcttgcaac gctaattacg cagtgtgagg agattttttc gcttatcaga atcgtcggcg
    35761 gcgcttatct cttatggttt gcgtggtgca gcatgcgccg ccagtcaaca ccgcaaatga
    35821 gcacactaca acaaccgatt agcgccccct ggtatgtctt ttttcgccgc ggattaatta
    35881 ccgatctctc taacccgcaa accgttttat tttttatcag tattttctca gtaacattaa
    35941 atgccgaaac accaacatgg gcacgtttaa tggcctgggc ggggattgtg ctcgcatcaa
    36001 ttatctggcg agtttttctt agtcaggcgt tttctttgcc cgctgtgcgt cgtgcttatg
    36061 ggcgtatgca acgcgttgcc agtcgggtta ttggtgcaat tattggtgta ttcgcgctac
    36121 gcctgattta cgaaggggtg acgcagcggt gagcagataa tttactctct acgtaatcaa
    36181 cctgatttgc cgaatcccga ccatcggcga tgattgcccc cgtaatgcgt ggtccttatt
    36241 tccgataagt ttacttgagt ctttagcttc ccatttcagc ccactctccc ctgtgtgggc
    36301 ttttttcatt ggtctgaccc gtcctgaata gccaaacaat ggcaatgttc cactttgcag
    36361 ggatgataat aaggggatct gattcagaag agttttgaaa agttggaccg ccatcgaggc
    36421 ctcgaacccc gtaccataca acaatcctgg ttgtcgtctg ctcttcctga tgagctaatg
    36481 gcggtttttg atatgagcct tacagcatat tttctgtcag gcttgagtgc gatgctaagg
    36541 tacagaaaag accccacaca aacatgatgg aattatgatt tttttcatat gaatgtcaac
    36601 ccacacattc acaaaacatc tgcatgagat cattttttat acaccgcata gctgcgaaac
    36661 cactgctggt gcttttttaa gcgaagcgtc gccgatcaca acaaaatatc ggcgaaaaag
    36721 gcggtggaat gccatttggc atacggatat acagatgaaa tgattacctg ctttgcgatt
    36781 cactctctgg tggaataaca acttgcaggt ggaacccttt ctgaactaaa gagtgtagcg
    36841 gattttaacg tgcttattta caggataata tctgtcacct aattcctgca ctctattcga
    36901 tatccattca tttaatcgtg cgtcatctct gtatgttttt acgtccagaa cagagagcaa
    36961 taacatcacc agtgattcag gaaaaacagg gtggatattt tttaagtgta ataataatcc
    37021 atgcgcaagc gcctgcttaa tgggcgcgtc tgtctccgga atagacgaca tttcgcgaag
    37081 ccatttttcc acacgcatac actctttccc tttagggaaa attctcagca aaggcatatg
    37141 gtgttcaaga agaatacatt tgcaatctgt tcgcactgaa tcgataaact gcactaattt
    37201 cttaagcccg gtaaagcgtc cagcctgcac atttgcttta atatcaagaa acgcatcatc
    37261 ctggtcatat acccaaactg tccctttttg attttcaagt atgcgaaaaa caaacttaac
    37321 cgggtgatca gggtaatcca tccagtcata aaatccaggg aaatcattaa actgcacatt
    37381 tatggtgagt gcgttatcgt ctgaccactc acagtctcgt acggcagcca tgagatgaat
    37441 gagtttgtcg tcatcgacta aatcatcacg acaatcataa ggataatcat aatattgttt
    37501 aatcaaaacc ttgaatatat cgacacagga aaccagggag cttttgatat cctctggtaa
    37561 ctgattgttt ttaaccgtat taagtatgct ttgttgcgta tatggcatca aattattgtg
    37621 cggagctttt aatgatatca tcgcgatctc tcttaaataa aaataaacgt cgcagataat
    37681 aaagctctca ttaaaccagg cactatcaga aatcgctcat aactaacgtg tgaagtattg
    37741 tgtactggag ggagttaatt taacaatttg aatttacgtt ataggtttta ctgctacttt
    37801 gatattattt cgctctcctt aactaaaaag ttaagtggag agcgaagtta atatgttaaa
    37861 ccacagcgta tttattaatt ttttcagtct gttagtgtgc gattatcgat aacaaaacga
    37921 tatttcacat caccacgcag cattcgctca taggcttcat taatttgatc ggcccgaatc
    37981 atctctatat cagccacgat gccatgttcg gcgcaaaaat cgagcatctc ctgagtttct
    38041 ggaatgccgc caatcataga accggctatc gcacggcgtt tcatgatcag gttgaaaact
    38101 tccggcgatt tatgcggtgt cgcaggcgca ccaaccagcg tcatggtgcc atcacgcttc
    38161 agcaaggtgg taaaatcgtc gagattatgt ggcgcagcta ctgtattcaa aatgaaatcg
    38221 aaactcttca catgagccgc catctcatcg gcattgcgtg agttaacaac ttcatcggcc
    38281 cccagggctt ttgccgcttc gcgttttgcc tcagaagtgg taaatgccac cacatgtgcc
    38341 cccatcgcgt gggccagctt aatccccata tgtcccaggc cgccgatgcc gaccacgccc
    38401 acttttttac ccggcccggc ctgccagtga cgtagcggcg aatacgtggt gatccctgca
    38461 cacaacaaag gagccaccgc cgccagctgc tcttgcgggt gacgaatacg cagaacatat
    38521 cgctcatgaa cgacgatctg ttgtgagtag ccgcccagag tatggcccgg ttcgtccggc
    38581 gtcggcgagt tataggtgcc ggtcatgtga tcacagtagt tttccagccc gtcttcacac
    38641 tcttcgcaat gtttacaact gtcgacaatg cagccgacac cgaccagatc gcccggcgca
    38701 tgtttttcta cctgatcacc aacggctacc acacgcccca caatttcatg acccggtacg
    38761 caggggtaaa ccgtccccgc ccactcggaa cggacctggt ggagatcgga atggcagacg
    38821 ccacagtaag cgatttcgat tttgacatca ttcggtcccg gttcacgccg tgtgatatcc
    38881 atcggttcaa gtggttgttt agcggaatat gcaccaacag ctttgatctt cattgtgttt
    38941 actcctgatt agctatgtgt atttggcgat gcctgatatg tcatcgcggc aaaacgcgtc
    39001 cattgaatat agccaatatt taaaccaccc gatccagccc caacatgccg cgaacctgaa
    39061 tgaaacagag cagcaacgtt ccccataagg ccatcgtcag gtaggggctt ataccgagaa
    39121 tattgaagcc gctttcaata atttgcagaa gaaacagcgc cagcaccata ccaattatcc
    39181 gcccgctgcc gccgtccgga ttaaccccgc cgagtactgc cgcgaggatt gacaccagca
    39241 agtacgattc gccataagag gccttagccg agttgagttt cgacatcatc aaaaatgccg
    39301 ctaccgcaca aagcaaggcg gaaatgacat aaacccaaat cagcacccga cgagtgttaa
    39361 tgccggagta aagtgttgct cgttcgttag acccaatcag gtaaatggct ttaccgagcg
    39421 gcgttttctc cagcaatatc cacagcccca gtgccactgc ggcaaacaac cacatcggta
    39481 gcggaatacc gaaccattgc gcatgattca accacagcac ccatgaggga taattggcaa
    39541 tggcgctacc acctgtcacc agaatattta tccctttgag caacgtcatc atcccaagcg
    39601 ttgcaagaat tggcgacact cgaataccag caatcaatac gccgttacat aaaccgatta
    39661 tcaccgccgc gccagcgccc gccagtaacg tagcgacaac cgtagcaata cccggtggat
    39721 actgagtcgc cacccacgcc atcaccagtg aacaggcgtt agcagtggct ataattgaca
    39781 agttaatgcc accgcaaagc atggtcaccg ccatagccaa cgccagaatg cccagcacgg
    39841 gcatttgcga agcgacggac tggaaattac ttatcgacca gaaaatctcc ggcatagtaa
    39901 tgctgaacgc ggcaacagtt agcaccagca ggccgatcaa gtaaaactca acgttattac
    39961 gccacgattt tttcatagca gactccggtt ctgatgctga ctccacgccg tggcactaat
    40021 gctggcaacg atgatgatgc cggtgatcaa tgtttgccag taagacgaga ctcccaataa
    40081 atttagcccg ttttgcatca ctgccaacaa caccacgccg agcaaagtac cggttaacgt
    40141 gccgcgcccg ccgagcaaac tagtgccgcc aagcaccacc gcagccagta ctgtcagctc
    40201 ataacccaga agagaatcgg gggcgacagt catcaccgtc cacgactgca ctacacccgc
    40261 agcgccagac atcaatccca tatagccgta gacgaaaagt tgcagtttca gcacgctaaa
    40321 gccgatgcga ctggctgatt cgcggttccc gccaagggca taaattttgc gccctacact
    40381 ggtgaaattc atgataaacg ctgtcagcag caccaccgta atcatggcga tcagcggcag
    40441 accaaggcca tagtcatagc catcagcatc ggtgtacttg aacaacataa ccccctgctc
    40501 aaaccacggc ggaaagtcgt acagccacac acctttactc aaccataaca gcaggccata
    40561 gaaaatattc agcgtcgaga tagtgatgat gatagaaggc acccgtaggc gattaaccag
    40621 taaggcgtta attaagccaa gtaaaatgcc gatgcagccc gccagtgcaa acgcgacagc
    40681 gaagttgcca ccaatttttt gcagcaacag caccatgccg tattgcgcaa tgatggtcat
    40741 tgctggaaac gaaatatcaa ttccaccgga aatcaacacc acaaacaaac cacaggcgag
    40801 aatagtcaac atggcatagt tattggcgag atcgtacaaa ttaccgaatg tcagaaactc
    40861 gtcgctgcgc caggcaaggc caacaaacag caccacaatc agtaatgcca gccagaattc
    40921 atggcgtttt ttcagttcag ccatttacca cctccgcaat cctggcctga gaactgctgt
    40981 caggcaggaa gctatgggtg atctgacctt tttgcattac caaaatccgg tggctttgat
    41041 accacgcttc ttcaatttca tcgcagatca tcaataccgc aatgccatga gccgccagat
    41101 cgctgataat gccgtatatt cctgctttgt tggcgatatc aacaccgact gtcgggctat
    41161 cgagaatcaa cagtcgtggc ccaatcgcca gccatttggc gatcgatact cgctgcgcgt
    41221 taccaccaga aagcgtattc accggaagat gaggatcaga gactttgatg gtcaatgcct
    41281 tgattagcct gtccaccagg tcacgaactt tagtttcact cagaaagccg aagcgatctt
    41341 ttaccttgtg aaaaacagtg ctaatgatgt tgtcttcaat agattgcgcc atcaccaaac
    41401 cgcgcgacat gcgatcttcc gaaacataac caataccgtg gcggatagcg tcctggttac
    41461 tatgcagtgt aaccggctgg ccgttgatga ggatctcgcc agcatccggt cgggtcatac
    41521 caaacagact cagacataat tccgtgcgcc ccgcaccaag caggccaaca atcgatacca
    41581 cttcgcccgc ttccacccgc aagttaatat ttagatattc cccatgacga cttaaattac
    41641 gcacctcaag caccggggtg ctggattttc cctgccacaa ctctcgcacc tgatattcga
    41701 actcctggcc ggtcatcagg aaaccgagtt gttttgtggt catctctgca gccggaaaag
    41761 tcccgaccag ctcaccatct ttcagcactg aaatacggtc ggaaacttcc atcacttctt
    41821 ccagacgatg actgacgaag accacgcaga tcccgcgttc ccgcaattga tgcaccacct
    41881 gtaataaccc ttgcacttcc tgatgggtca gcgaagcggt tggttcatcc atcacgatca
    41941 accgtgcgtc ctgcgccagt gcacggcaaa ttgctaccag ctgacagcgg gcaattgaaa
    42001 gctcagaaac gagcgtatcc agtggcaatg tgacgttaat actggtcatt gccgcctgcg
    42061 ccacctcacg cagacgacgg cggttaacaa acagaccgtg gtgatagtga ttcacggcga
    42121 tattctccca gacactcagg ttaggaaata aagagaggtc ctgataaatc acctgaatcc
    42181 cctgcgccac cgaagcggcg ggtgtcagct ttgaccagtt tttcccacca agcgtaattt
    42241 ccgctccctc atccgggcga taaacaccgg acataatttt aatcaacgtc gatttaccac
    42301 agccgttttg cccggcaagg cagtgcactt cacccgacat aaagtcgaga gaaacatcgc
    42361 gcagcgctcg cgtggcatga aacgttttgt tgatatgacg aagggaaagg aaggtttcca
    42421 taacatcccc tggcaataag aatcggggct ttcgccccga agaaatggat taataaagtg
    42481 agtcgatatt gtctttgttg accagtaata ccttatggaa acggataatt cgcttatcca
    42541 tatctacgtc ggccttaccc agattctgca tttctaatcc aggtttgatc tcttcaccct
    42601 tcagcaacgt gctggcgacg gctgcgagtg cgtaacctgc agaggccgaa tcgtaagtaa
    42661 tcccttcggt gatatcacca cttttaatca gtgatgccgc ctgtgaaggg atcatcatgc
    42721 catagactgc gactttattt ttcgcccgtt tctctttcac cgcacgtccc gcgccaatcg
    42781 gaccgtttga accaaaggag acaaccgctt tcaagtcagg ataggttttc atcaggtcca
    42841 gtgtggtacg acgtgagtca tccacactct cggcaaccgg catgcggcgg gtaacttcat
    42901 gcatatccgg gtaatgctct ttctggtatt tcaccagcaa gtcagcccat aagttatgct
    42961 gcggcacggt caaactaccc acgtaaatca catagccgcc cttgcccccc atgcgtttcg
    43021 ccatatgctc aacatattca gcggcaaatt tttcgttatc aatgatttcg atatcccagt
    43081 tagcacttgg ctgaccgggg gattcgttgg tcagaaccac aattccggca tctcgcgctt
    43141 ttttgaatac cggttccagc acgttggcat cgtttggcac gatagtaatt gcattaacct
    43201 tacgggcgat taaatcctca ataattttaa cttgttgcgg agcatcagta cttgaaggcc
    43261 ccacctgtga cgcattaaca ccaaaggcgt tacccgcctc aaccacacct tcgcccatgc
    43321 gattaaacca cggcatacca tcgactttag agatattcac cacgactttt tccgctgcct
    43381 ggagcggcgc agaaattagc gcagcgccta ataacagcga agacaccata ttgataacaa
    43441 aacgtttatt catcatttca gcctcattgc agggtagtca ggaagtgttc gcccaaatta
    43501 gcgcgctgga gagaataaga attttcgaat atttttatgc agaataaaca gccctatttc
    43561 aggcagctat taaaaatatg gttataaata tggacaaaac gggcagcaac aataagtgtg
    43621 acattcatca cgtaaaaaat agcaaaataa acaaaattag cgatatttta tgtgtgtgag
    43681 tttatttcgc gtagataaat agttatctac acaacgtcaa taagggcata ttcttcaaat
    43741 ataacgtcca tttctggtga atagcttcct tctgcctgga aaaaacgacg gtgttcatta
    43801 cgccaatatt caagactgag gtcaccttca ccttccttac gggcaagctc cacggtgaca
    43861 tcagaaaagc gcagtaaatg caggccaatc actctgataa cacatacagg ctcaccacgg
    43921 ctattttcta caatcttata ctcaccaatc atcggaaatg cgttgtcttc aatacatccc
    43981 gccagcgaac cacaggaagc tgttttaaca ccagtcgcaa tcaacgttgc gaggtgatcg
    44041 gccaacgctg ctgaatcgcc aaaagtacaa aaggaagcat ggggatattt ttgtgagaat
    44101 ttttctttta tggtcatggc aatatttttg cgattacgga aaaacaaatc acatcttaat
    44161 tactgttggt aaccatcagc aagagtatca ttggtttttc gatactgaat cttttttatc
    44221 cgaagcaggt cacatttatg gcctgataaa tacctttgtt aaaactcact tttgtgtatt
    44281 tctctcaaat aatcccctgg aaattatgcc gcgagattat aaagttcagg atcattcatt
    44341 agcaaaccat tcccgcccca ctccctgggg cttaaatctc ttacaattca ccgttgcagg
    44401 cgtttcaaat gcgcatcaac ctgccacact cccccacacc agttgccctt tatggaacgt
    44461 tgcggttctc ggcgagatac gcgccaccgc ctcggcggaa caggaggcgt ccaccagcac
    44521 aaagctggca tcatcctgcg cttttggcca tacacgctcg cctttttcat tcagcggcaa
    44581 tacatcgcca gtggctagaa acagcgaacg ggagaggttc tgttcgttag gacgaatata
    44641 gagctgcgcg tagagattgg ctttttccag catgtcgccc agaccataag gcgaccagtg
    44701 gtcgataacg ctgtcagtgc cagtcatcac ttttacgcct ttgtcgtgca actgtttgag
    44761 cggcatatgc agcgtgccaa tcggcaccgt cgaggcgata gaaatttgct gcgccgccat
    44821 ccgattcgcc agcgcatcga cctgttgttc gttgagcgta gccagcgcaa aggcgtggct
    44881 gatggtcagc ttacctttca gttgcggcgt tttctctacc gtttcaacca tataattgat
    44941 ggctgccacg cccgacggag tggtttcgtg caggtgaata tcgacacctt tgtcgtagtc
    45001 cagcgcaatc tgaaacatgg tgtcgaggga tttttccatc gcgccatcga cactggtcgg
    45061 gtccagcccg ccgacgtaat gcgcacccgc ctgcatcgct tcgcgcatta agggttccga
    45121 tttcgacagc agcaaaccgt gctgcgggaa ggcgacgatt tcacactcga agcccgcctg
    45181 acgtcgcgcc agcaccgcct gcaaattctg cagatttttc aggccggaaa ccggttcgat
    45241 attgcagtgg ctgcgggcaa tggtggtgcc tttcgactgc aatagatcga tcagtttttc
    45301 tgcccgttcc tgcgtgtacg gttgcagttc cggcagcatt ttctgctcaa gcttgatcat
    45361 gtcctggata gtggtgcctg ccggacgatt gagcgagcgc cacggcccgc cgtagaaggt
    45421 tttgtcgaga tgaatatgca tgtcgcgagt ggtgggtagc atcaacttac cgccagcgtc
    45481 atagtgcggc agcgtggcgt ccggatgctg cttgttctcg cgcagggcga caattttgcc
    45541 gtcctgaatc tccacggtct ggcgcgccgt gcgggtctgc accgccacac cgttttcgta
    45601 gtcaaaaccg gtttccagca gcacgttatc gagatagtaa tgcgggtcag tgattttcat
    45661 ggtgttgttc gcttcgcagt ttagggtgcc agctaccgcc gcatgggcga gcggcacaga
    45721 ggtaccaaac agcgcggcgg cggtgaccat cttaccgctc tggctcagaa attcacggcg
    45781 gctattgctt tctttcatcc tacgtcctta cgttttaata atatgggtgc ccgtttcacc
    45841 gcgcagcgcc gcaggcaggc attccggcgt ggtgataatc acttctttgc cgccctgctc
    45901 caggaacgtc aggctggcga tgatttttgg caacatgctg ccgggcggaa aatgcccttc
    45961 ctgcatatag cgagtcatgg tggcaatatc cacccgatcc agcgcctgct gctgcggttt
    46021 accaaagtgg atacagactt tttcgacgcc ggtggtgatc accagaatgt cggcgtgaat
    46081 ttcacgggcc agcagcgcgg tagagagatc tttgtcaata accgcgtcca cgctttggta
    46141 atcccccgct tcagtacgca ctaccggaat tccaccgccg cccgcgccaa ttacgacaaa
    46201 accttgttgg atcagcgcct taatggcggg tgcttcgaca atacgtttcg gttccggcga
    46261 ggcgaccacg cggcgatagc cccgtccggc atcttcaaca aaacaccagt cagggtttgc
    46321 cttttgtagt ttgtcacgct ggctttcact gaagaatgca ccaatgggct tggtgggatg
    46381 ggcaaaacct ggatcgtttt tatccacttc cacctgagtt accacggtga cggctttttt
    46441 ctcgccgtga cgcgccagcc ggttattcag cgcctgttgg atcagatagc cgatgccgcc
    46501 ctgcgtatct gccacacagt tcgccagcgg cgttaagggc agcccttcgc gctcgtgggc
    46561 aatctccgca cggcgtaaat ccagcccgac ctgcggcccg ttaccgtggg tcagcacaat
    46621 gtcgtaatcg gaagccagca tttccaacac cgtatcggcc acggctttca ccgcctccgc
    46681 ctgatgctca atcgactggc tggcgttatc tttgataatg ctgttgccac caatggcaac
    46741 gaccacaagc tctttcatgt tgttatgtcc gctacagagg gtgattaggt tatgaccaac
    46801 gccagactgc ctgatgcgct acgcttatca ggcctacaat tctcgggcca tccgtaggcc
    46861 ggataaggcg ttcacgccgc atccggcatg aacatgtagg tcagacgccg taacgttcgc
    46921 accagccaag aatggctttc tcgaagcagg caaaaggtgg atgcacaatg cccgcgccaa
    46981 tcatgccgac gcccgcatct ttatgggcga tggcagtgtt gatcaccggc agaatgccgc
    47041 tgctacccac gcgggtgatg tcgattgccg acggcacgcc cataaaaccg agcagcggaa
    47101 tggtgacgtt ggggttttca ccgagagtga tttcacgcat ctggcgagag aaatcgatag
    47161 cctcttccac cgtgccgccc accagcgcaa cgattgcagg cgcggtcgcc atagcaaatc
    47221 cgccaatacc gtaggtttca gtgatggcgc tgtcgccgat atccagcccc gaatcttccg
    47281 gcttataacc ggcaaacatc gggccgatga cctgctgcgc cggaccggta aaccattgcc
    47341 ccggtaatcc gctgacccgc aggccgaact cgacgccgtt acgcgccatg gtggtgacca
    47401 cggtgctgta ttcgatgccg tgcgccgcat ccatcgccgc tttacacatc gccatccacg
    47461 tcgggccgga gaagtagtcg ctgctagcga caaactcaaa cacttcgcgc tgtttctcca
    47521 cggaataacc tgcctgaata attcccggcg tcagcgcctg aatcagcaac gtcgtcccgg
    47581 cgttattgcg gttatggcac tcatcgccca tatgcagcgc ctgcgccagc attaagcgca
    47641 gatcaatctc gccgataatt ttcatcgcgt cgcgcagcat tggcccctgc acatcgcgca
    47701 tccagttgag gcggtcgatc acgctctgat cgttggctcc catacgcaaa atcttcgcca
    47761 tctgctcgct catattggtg taagcgatgt tgccgtaggt tttgtttttt acgatgtgca
    47821 taaacatcga ggcagaggta acacccgcca tcgatcccac acagtcgtgt tcgtggcacg
    47881 gcgagaaggt gatctccccg gaggccgcca gttcagccgc ctcgtcgaga tctttcgcca
    47941 gcccttcgaa caccaacgcc ccggtgaccg cgcctttcat cgcgccgcac attttttccc
    48001 aggtgatcgg cggcccggcg tgaagaatgg ttttcggcgt catgcccggc accacgttaa
    48061 tcgcctgatc aaaaccaatc agcaccggat gcgactggat aatgcgctcc agcgcctgct
    48121 ggttagcggc ggcgattttg tctgccagcg gcgaatcggc aatgttatcc agcgcctgca
    48181 ccacctgcat attgccctgc cccggcggcg tccagtcgag ttgagtcact tctacatgct
    48241 gctttttcag gtcatcgcta aacatggcga tgccgacgtt aataacgttc aatggttggc
    48301 taaacagtga ctggctcatc aggcttcctc ccctttgcag ataaattcac gcgccagcaa
    48361 tccggtattg gtgctgctgc tcgccagaat cactccggca tcaagcagca tctggctttg
    48421 ttgttctaac gatggcgtat caagatcggt acccagcaca taggcgagaa tgatcaactc
    48481 gcgtccttcg gcagcggcga tcgctttcgc ttctttgatc gcctcgatgg tggagccgac
    48541 cggatcttca tgcgatccaa atccgagcac aaaatccatc acgatcaccg ccacttctgg
    48601 atcgcgcgcc tcttcgatca agcgactgat gcggttggtg gggtcaatca ttggatgcgg
    48661 cttgccgttc gtgaagtcgt catcgccaaa gtcgaggaag gtgtgtttga tgctgcggtt
    48721 gatatcttgc aggcggaatt ccggatcggg ctgaatgttg ctgtagacat cgccatgttt
    48781 ttccatcacc gcgaacatgg tttcgtcgca cagcgtgccg ccgcagaaca ggccacgaat
    48841 gtatttctgc tgcggttgca gacgcgcacg cacatcagca atcaacggct ggttaagcgt
    48901 atgcaggtcg agattttcct gtttcacgcc ggagagcatc accgctttta gcgccgcctc
    48961 tttgctgccg cgggcaaact gtagcccctg ctcatccacc ggcgtttcaa cgcgaccgag
    49021 gaagcagacc accaccggct tgcggcaggc acgtgcgcgt tccagcactt tgcgggccac
    49081 cgcaggcgcg ggaggttttg aaatcagtgc gataatttca gtttgcggat cgttttccag
    49141 catcccgatg gcgtcgagca tcatcaggcc gccgattttc tcgctcaggt cgcgcccgcc
    49201 ggtgccaatc agttgtgaaa caccgccacc aaattcatga atgcggacgc tcagctcctg
    49261 gctgccggtg ccggatgcgc caacaatacc gatattgccg cgacgcacgg cgttaccaaa
    49321 acagagcgcc gcgccgttaa taatcgccgt gccgcagtcc ggccccatca tcagcagccc
    49381 tttttcgtgg gccaactgct tgagcgccag ttcatcttca actgagacgt tatcggaaaa
    49441 cagcatcacg ttgagatcgt tttgcagcgc ctgacgcgct tcgcgagcgg caaacagacc
    49501 gttgaccgaa atcaccgcca ggttactttc cgggatatgc tttttggcgc tggcaatagt
    49561 ggcgtaacgc gcctcgtgcg agccgctttg cgctttggtg ttgaacagtt cttcaatctc
    49621 caccagcaac tgctcgttgt ccgcacccga tttaccattg atgacaatca tcaggtcgcc
    49681 gtttttcgcc tgttccagct ccggcgtcag cagtcctaaa tttttcagta cacctttgtt
    49741 catttcggtc gccatcgcca caaatgcctg ctcgacgccg tcgagtttat ttgcacgcgt
    49801 ggagatagac atcagcgaga cagaatcaaa ataggtattc ggtttaatga ctattttaac
    49861 tgacataatt ccctccgtag gtttggctca gcgcacaacc atcggccatg ccatacagca
    49921 tgtcgcagcc ggaactggag ccaatatgtt taattttttc tattgcctga gttgcgttcc
    49981 cagggatgtc ataaataatg ttgtgaataa aacgatgaat attttcccgg caacgttgtt
    50041 gtaatgcggc ttccagcgtt atagcactta ataatgtggt gttatttctg ccgcgctgca
    50101 taccgagata aaattcctct ttgtattttt ccgacggatg cccgggaata aataaaataa
    50161 gcgccagacc tgttaaataa tcgtcggctg atggcgtaag gccaatccct aacccaatca
    50221 tactggcgac cgctgcggcg atattttgtt tttcgtttac tgcctgaata actgcccgtc
    50281 gtttaatatg tagttcttta tttaacgcct gataaaacgg attatcgccg cgataattaa
    50341 acagggtttc attttccccc aggtgctgat gaataatgtc gtgccactgt agccaggcga
    50401 taaggtgaaa attcacagcg gtcatttgca aggttgggga cagccaacgc cgacaacgac
    50461 tcatctctat atgaagatgt tgaccaaccg taataccttg atcgtcaaac tgaaccttat
    50521 caccatgccg gaacagatca tcaaagtgag tgagtgccaa ccgacaactg tttggcgcat
    50581 tgtcgtactc ttcacaaagc agcgtcagca actgctggcg agccgggagt gcgatattta
    50641 ccgctctggc aaagacctgt tcaacctgtc cttgcccccg ctgcgccaga aaatccgcat
    50701 ccgcagttag cgcccacaca caccgtcttt ctggtgtgtt gatagcaagc ctcccggttt
    50761 atgcacctgc ggcaatcagt aactgcgcaa tctcttcaaa gccctgttcc cgcgccagtt
    50821 ccagtggcgt tttgccatat ttatcggtca gatgcgggct ggcaccgtgt tccagcaata
    50881 actgcacaat cgcctgctgt ttaataccac catcattaag cacaatcgct tccagtagcg
    50941 gcgtccagcc tacatggttg gtctggttaa cgttaatctc cgtatgcgcc aaaagctctt
    51001 ttacaatact taaatggcct ttttcacagg caggcgtcag gccgacaccg ccaaaacggg
    51061 ttacgcaatt aagatcgggt ttagccggta aaataattcg tagtagcgtt aaatcatcgt
    51121 tcagacagct aattaaaaaa ggatttaaac aggtatgatc ttgcttatta atatccgctc
    51181 cggcatcaat taatgcctga acgcaagcat attgctgata taaacttgcc agcgtaattg
    51241 ccgttttccc ctgacgatcg caggtattaa tatcgacacc cagcgcaagg caggttttta
    51301 ctttatcgat atcaccctgt tgtgcagcca ataaatagtc ggcaggtaga ttttttatac
    51361 tcattcacat tcccctggtt tttgtggctg ttttcgatga attgagaata gcaacgataa
    51421 tctgataaaa gaacccgctt aaatttaatt cgctgtattt cgcctttttg ttgaatgatg
    51481 ttcaacaatc ttttcgcagt gtgcggtagt gcaatatttg tttctttgac ccgataccct
    51541 ttcactcaat caggttttat ttctctatag tctgcacatg taagagtagc tttcgtccat
    51601 gtctaaccag aggggaacaa gatgaacgga ctcacggcaa cgggtgtcac cgtcggtatt
    51661 tgtgcagggc tttggcagtt agtgtcttca catgtcgggc tctctcaggg atgggaatta
    51721 ttaggaacca ttggcttcgt cgccttttgc agtttttatg ccgcaggcgg cggtaaatcg
    51781 gggtttatca gaagtctggc ggttaattat tccgggatgg tgtgggcatt tttcgccgcg
    51841 ctggctgccg gttggctggc ctctgtaagt ggactgtccg cgttctgggc gagcgtaatt
    51901 actaccgtac ccttctcggc ggtagtggtc tggcaagggc gtttctggtt actgtccttt
    51961 attcccggtg gctttctcgg catgacgctg ttttttgcca gcgggatgaa ctggacggtg
    52021 acgttactgg gttttctggc gggtaactgc gtggggataa tttcagaata tggtggacaa
    52081 aaattaagcg aagcgacgac taaacgcgac gggtattaaa agttattcaa aaatgattat
    52141 ctatctttaa ccgatacctc tacagtcata gctggagaaa ttcatagtag ataatggcga
    52201 gaatggttta atgaactcaa tttttaccga agagaatctg ttggcattta ctaccgctgc
    52261 gcgttttggc agcttcagta aagccgcaga agagttgggt ttaaccactt ccgccattag
    52321 ctacaccatc aagcgtatgg agacggggct ggatgtggtg ctgttcactc gcagtacccg
    52381 cagcattgag ttaacggagt ccgggcgtta ttttttccgc aaagctaccg acctgctgaa
    52441 tgatttttat gccatcaaac gcagtatcga tactatttcg cagggcatag aagcgcgtgt
    52501 gcgcatttgt attaatcagc tgctttatac gcccaaacac accgcacgct tgttacaggt
    52561 gctgaaaaag cagtttccta cctgccagat taccgtcacc accgaagtgt ataacggcgt
    52621 ctgggatgcg attattaata atcaggccaa tatcgccatc ggcgcacccg atacactact
    52681 ggacggcggc ggtattgatt acactgaaat tggcgcgatt cgctgggctt ttgctatcgc
    52741 cccggatcac ccgttagcct ttgtgccgga acccatcgcc gaaagccaac tgcgtctcta
    52801 ccctaatatt atggtggagg acaccgcgca tacgattaat aaaaaagtgg gctggttgct
    52861 gcacgggcag gagtcaattc tggtgccaga tttcaacacc aaatgtcagt gccagatttt
    52921 aggtgaagga attggttttt tgccggatta catggtccgt gaggcgatga cgcaatccct
    52981 gctggttacc cgacaaatcc ataatccgcg ccaggattcg cgcatgttac tggcgactca
    53041 gcattctgct acgggtcagg tgacgcagtg gattaaaaaa caatttgcgc caaatggcat
    53101 attaacgggg atctatcagg atttactgca tcgggagaat taatcgctgc caacctcccg
    53161 atgcgctgtg aatagcggtt tacatcaacc acctgctttc attacccatt cagagataaa
    53221 tttattaccc ggtactggcg gagagtacaa atagccctgc aaaaagtgaa cgcctttacc
    53281 gatcattaaa tccgcctgct cctgggtttc taccccttcc gccacgatac tcagaccagg
    53341 cttacgcgct agttcgacaa tattgtccac aatatgaccg gagatttcgt cgacactcgc
    53401 catttgcaca aatgacttat cgatcttaat aaaatcgacc gggaacgcct gcaagtaacg
    53461 ataggtcgca taacccgtac caaagtcatc cagcgcaaag gtaatgttgt gctgatgaag
    53521 gctgtcaaat atcgctctgg cttctggcgt taccggaata gggttacgtt ccgttagctc
    53581 gagaaccagc ttgattttat cgttacctaa tttcttaacc aggttcagac actctttttc
    53641 aaatcccgct gccaaaaaac aacccgccga gacgttgatg ccaatatgga aattgtccgg
    53701 cagcaaatgt tttaccggca tcagaatatc cgcagtctgt ttcatcagtt ggcgggtcat
    53761 tatgacaata agaccggatg actccgccag aggaataaac tgatccggtg ggataattcc
    53821 cgtttgtgga tgttcccagc ggacaagtac ctcacagccc gtcagtaccc cagtctgcgc
    53881 gcagaaaacc ggttggatcc acggtttgaa ttcgtggttt tccaacgcca gactgatggc
    53941 ttcaggcgtg acgatatggt gacaacggtt atcagggata taaaaattat ttttatttcc
    54001 cgtgccactg atgacttgcg gatgatattg aaaaaagata tcccgccaga aggtaatatc
    54061 actctggatg cctagttttt catacagctg cttcttttga tgtgaaattg tctttgcact
    54121 gcgatttctg tactgtgaga tttctgtaac cgacattccc tccattagca gcttgagtaa
    54181 aagccgttct ttttcggaca cgataccagg caacgtcaaa ttcaccgttg ccccgaactc
    54241 ttccaaaaaa acactcaaat catgcgaagc cattgaacac accttgatta aatcttgtgc
    54301 gccagctaaa tctgcgagtc ttcactctga ctgataaagc tggctgcaag aaaatgacac
    54361 agagagccga attatctccc gttcggctct ctctcctttt actcattatt cagatcagaa
    54421 ttgccactta atgccaacca ttgcagaggt gtcattatat cccctatcgg caacctgaac
    54481 gcccacattc ccccagacat tcaggttggc atttagctgt ccttccaccc cggtttttat
    54541 ctcagcaata tttcgggctc catcctgagt gacggacacg ccatccatac tggtgctgaa
    54601 atccttactg ttatgcagcc agttcacttc tacaaacggc tggaactcgc gggatttacc
    54661 gtcatccatt ttatggtggc tcttcagcca ggtttttacg ccaagtcggg tttgaacatt
    54721 gccatcaccg ttgctatgaa cgagggttcc gttgctttcg cggtgcttat cggctttgac
    54781 tcccatccag gtaacctgtg cttgcggctg aacataccat tcattacgcg ttccctggct
    54841 gccattaaat tcagctaatt tgtgtttgta tccagcttcc agtgaagcgg taaatccttt
    54901 tgatttatag gattcacttt gtaagtcatc ccctttcact gtgttatcaa accagctgta
    54961 ctgcgcccaa ctgtcgagat acgcgccatt acgcgactcg tcatcggcat accaggtggc
    55021 atagaggccg gtgctatatc cgttcacact cgcttttgca cgataaccgg ttcgcgagga
    55081 aatggttttg ctgtcgctgt tgccatatcc cgccatgacc ccaacatgcc agctgtcgct
    55141 gccgttttgg ctccactgcg cgacatcgcc tcccagttgc agaacatagc gattgctttg
    55201 ggttttcagc tggccgctgc catcacgcca tttattatga ccaccttcat ggcgcatcca
    55261 catagtggtt tgtttctgct cacccgtcac catgtcggta tagtacgtat tacccagacg
    55321 ctcatgcaga cgcgtggtga acattgtatt cgctgctgca aggttcgcaa tgtagctacc
    55381 cgcctccggt cgcaggtcat tatccacatt cagatccgga cccggcgtag gtgtcgggtt
    55441 agggttcggc tctggattcg ggtttggctc cggattcggc atcgggtctg gctccggctg
    55501 cagttcagga gaatcactgc cgctggtcag ataccagtta ccactatttg ccccttgtcc
    55561 acgcgcgaga gtgtagtcat acgcccccgc aacgatacga ccagcctgaa caaattcgcc
    55621 ctcagactta ccgtctacat ggataagttc gataccgtta agggttttcg cacctgtacc
    55681 gccagcgtta ttcaccgtaa cggcagtcgt gccgctagta tcgccctcga caaccaattt
    55741 atcggttact gagtcatcgc cattcagtac cgtattcatt actagttgac cgttattacc
    55801 gtggtagttc cccttcacca ctaacacatt gccagcttgc tgtcctgaca ttccggtcat
    55861 cagcgtaccg ttgttggtca gattgccgcc cagggtgtaa ttcacagacg cagccgctct
    55921 tttttcagtg ctattattca acgccagggt accgttgttc tcaacgtttg cgctacccaa
    55981 tccctgcgca tgacttacca ggacattagc cgaattagca atgttcacat tcgccgtcag
    56041 gttgctgtta tcgcccgcga tgtccagcgt gccgccctga atattcagct ctccgctccc
    56101 cgttaacgaa ccggttgaag tgcccccgtt ggtaaccgtc agactgccgc catttaagct
    56161 cagcagtgaa tcggcgctgc cattgagcgt gcccacggtc tggctgtgac cattcatatc
    56221 cagttcagtc tctgccgcca gacgcagttc atgggtatta cccaacacgt tatcgttatt
    56281 caacaaaagc gtcccactgc gcagatcggt aacaccggta tagtcgttgt ctttgttaga
    56341 aagcgatacg gtctgaccct tctggctgct gaatgccagg tcaccactgc cggtaatctt
    56401 tgcgctgaga tcggcggcat tctcgctttt accgttagag ctcagcacca gagcatcgct
    56461 gtcggtagcg tgtaaatcaa gctgggtcag gccgtaaccg atatacagac cgtcattgtt
    56521 taccccactc gtcagacgat agtcgtaagt gccgacggcc gctttttgcc cattctgctg
    56581 aatatcacgt tgggcgctgc tggagaggat ttgcccattt ttatcctgca attgcagatt
    56641 gcccgcatcg cccagaactt ccgcaccttg cgcatcaacc agcttaatgg cgctgttacc
    56701 atcatcgact tcagtgagcg ataacgcaga gtcaatatca cgagatactg agcggacaac
    56761 gtcactgtca gaaacctgaa tcacaccctc gccgcgcaaa tccagcgttt tactaacgtt
    56821 gaccgtccct tctgtcatct gtgcacctgc tgtcagggca ccaaaatcaa cggttccgcc
    56881 ggagaatccc aaagtgctaa tggagtgagc gcccttaccc actgttacct ggcttccctg
    56941 acccgcagtg atcccgcctc tctgcagaaa ctcagcattc ttttccaggt taaatgtcgt
    57001 gttctggaac gccagtgaac cctggaacca gtctgtataa gcaaactcat cgaaggagaa
    57061 ttcgtgattc cccatgttta tttcaacaat acctctacct tgaagatcgt tatcaaatac
    57121 ccaatcttta ttggcattga tttgcagttt gccatcaaca tccaggtccg cacggccgat
    57181 gttcttgtct tcagaaataa ccgcgacagc accagaagcg acatcaatat atccgctgaa
    57241 tgcatagtta ttgccggtca gtgccactgt cccactgtcg atacttattg taccattgcc
    57301 ggaaatctta ttggcaaagg tcatgctatc gtcagccttc aggtgtaatt tcccgcggtt
    57361 gttgatctct gctgtaccta tatttttggt gatccctaaa gcgccatttt gctcaatttt
    57421 gactttacca ctaaaggcag agttatcacc ggttaagtaa gtcgtgccat tgagcgcatt
    57481 gatatagccc aggccctcgc tgccactgac aatatccgcg gaaatagtcg cgtttttatc
    57541 cgtgtggttt aaggtgattt tcttatcgga tgtctttgcc cacacaaact caattttttc
    57601 ggcatcaata atccctgcag ccttcgcagc acttccttcc tgtgcaccta acgctaactc
    57661 agagttggtg cttaaactaa ttttcggtgc ggatatttta cctccatctg atacgactaa
    57721 tgttgtatca gaacgttggt cgtaataacc caggctaata tgttcacgag cattaacttc
    57781 accaccgtta ttaaccgaca ggaactgatt accgccatat ccgccgtaaa cgcccataaa
    57841 gaaggtccct gcatttacct gactaccttc gccgcttaca ttcccctgcg tataagtcag
    57901 acgactggtg cccttaacat gaattttgcc gccattggag acattaaaat agccttgatc
    57961 atgtctcatg cctgagtttg actcatacaa atcatcagca tataactcgc tgccagcatt
    58021 ggagacattc aggaaccccg tacctttcag caaaaagtta agcgggccat tgattgttac
    58081 ctttcctccg gtaatatcca acccaccacc agaagcgaga gtcagacgcg gacgggaagt
    58141 atctgtcgta ttgattacca gattggcatt gtcagtcacc gtaatacggt aatcgatgct
    58201 ctggttctct ttgccaaaat caatagtttt atcctggttt acgatgacat catcggcgaa
    58261 taccagagat gaatataaac ttatcgcgct aattaaaaca gcccgggact tttttccttt
    58321 tactctgctc cccagctcag agcagacgct ccaacagtta cgggacctgt tccactttag
    58381 ccgatatatt ttattcatca caatctccag caatacatcc aaataaaacc aagattgcat
    58441 aaacagcaac gcattaaatg ccattctggt tactgttcat cgcatataaa aattcaaaaa
    58501 ttttgcatag cattggcaac cacatatctg agtatgtagt tcagagggta ataaaatagc
    58561 aagttattac ttaaaactta ttttaagtta acactcttaa ctttatcatt acattcctcc
    58621 cagtctctaa tacctgcgca ttatatgtat ccaggaactc aaatcaactc aaaatataaa
    58681 caaaagcaag aggaatacat ttaatataca gccgataaaa gcggtaactc ttattaggta
    58741 tcaaaatata agaatcgtct tattaataaa tatataacac tataggactg aatccattca
    58801 gctaatacaa aataaaatcg ttaattaata aaatataaaa tcatgaagtg atacaagagg
    58861 aattgcagga tatgacatga caatcatata taatatatgc ataatttttt acagcgagtc
    58921 aatgattccc gcgccatatg cagacatggc gcggttttat gcaaaaacaa tcacgcgtcc
    58981 gggaacatca cgctatggcc cggcgcttca cgatggagat gaataaagtt aaggtgccgc
    59041 tcgtactggt caagaatatc ggtgatcacc tgctctttgc tgtagtccat caggtcgttg
    59101 ccctggctgc cttctaacag gaaggtttcc agccggtagt aggtcgattt accgctacgt
    59161 gcgcggtagg taaagcccgg caccgaatat tgctgcggcc aaatctgata gacaaagttt
    59221 tgttcttcgc ccatatgcac caacaaatcc agatgcccca actgttgtcc ctcttccggc
    59281 ggcaggcttt ttagctccac gtatgcgccg cgcaaccgca gctcctgcgc cacttcttcc
    59341 attgccgggt aacagaccgt ctccatcatc tgtttagtgt aacgcgtgcc cggataattc
    59401 atcaggcgcg agagacgttt tttccagctc aggcgatcct gaagccccag cggtcgcggt
    59461 gcggtatcgc ggttggcact ttcacggcgg taatcttcta ccttcagaga tttatacaac
    59521 cccgccatca cgaagaagat cacaaagctg aacggcagcc ccataatcac cgtggtgttt
    59581 tgcagcgcgg atatcccgtt ggtcatcagc atgccgagcg tcagcaggcc aatcgccacc
    59641 gaccagaaga cgcgcagcca gccgggggcg tcgctgttga tatctttaag ctgcgaggtg
    59701 aaattcccca gcaccagcgc accggagtcc gccgaggtca cataaaacag caggccagta
    59761 atggtggcga cggaggcgct aaaggtaaac gccggatact gcgccagcag gctgtagaag
    59821 ccgcgctccg gatggaccat cgcttcctcg gcaaatgccg cgccgccgtg gatgatttca
    59881 tacagcgcgc tattgccgaa caccgagagc cataacagcg tgaaggtaaa cggaataatc
    59941 aacgtgccca gcacgaactg gcgaatggta cgcccacgcg agatacgcgc caggaacaag
    60001 ccgacaaacg gcgaccatgc cacccaccat gcccagaaga agagcgtcca gttattcatc
    60061 cactcaactg gacggtcgaa ggcaaaactg ttgagcgtca tgcccataaa gcgattcaca
    60121 tagtcgccaa cattcagcac cagcgcatta agcaggaacg aggtgtcgcc cataaacaat
    60181 acgaacagga tcaatcccag cgccagcgca acgttaagtt ccgataacac acgaatcccc
    60241 ttatcgacac cggaggtgac agagatcgtg gcgattatca ccgacaaggc gatcagtgcc
    60301 gcttttgccg ccatcgaatc gggaatatca aacagtacgc tcaagccata gttaagctgc
    60361 accacaccga taccgagcgt agtggcaata ccgaagatgg tgccgatcac cgctgcaata
    60421 tccactgagt gccctatcgg cccgttaatc cgtttaccga agatcgggta cagcgccgag
    60481 cggatggtga gcggcaaatt ataacgatag ctaaagtatc cgagcgccat gcccatcagc
    60541 gcatacatcg accagccggt taagccgtag tgaaacagcg tccagaccat cgcctgacgc
    60601 gcggcctcaa tcgtctgtcc cgcgccttcc ggcggctgca tatactgcgt taccggttcg
    60661 gctacggaga agaacatcag gtcgataccg atcccggcag caaacagcat cgccgcccaa
    60721 ctcagcaggc tgaattccgg tttggattgt tctggcccga gcttcaccga accaaaacgc
    60781 gaacaagcga tacagaccac aaagacaata tagagcgttg ccgccagtag atagtaccaa
    60841 ccgaaggttt tagaaaccca gtccagcgtg cggccaatcc acagggccga gaagtcgcga
    60901 aacaggatcg ttgtcaggga aaacaacaaa atcagtccgg cggaggtgta aaacaccacc
    60961 ggattgattt tgtccttttc cctgctgtgt gaaaggtctg tcatccagta tccccactgt
    61021 tattgttacc atttaaaatc aaattcgtaa caattaagac acattttata ttgaacgtcc
    61081 aatcaataac cgctttaata gataaacaac gctgatgaat ggagtggcga aaatgcccaa
    61141 attggggatg cagtcgatcc ggcgcagaca actgatcgac gccacactgg aagcaataaa
    61201 tgaagtgggc atgcacgatg caacgatcgc gcagatcgcc cgccgtgcag gcgtttctac
    61261 ggggatcatc agccactatt tcagggacaa aaatggtctg ctggaagcaa ccatgcgcga
    61321 tatcaccagt cagctgcgtg acgcggtttt gaatcgatta catgcacttc cgcagggcag
    61381 tgcagagcag cgattacagg cgattgttgg cggaaacttc gatgaaacgc aggtgagcag
    61441 tgcggcgatg aaagcctggc tggcgttctg ggccagcagt atgcatcagc cgatgctcta
    61501 tcgtttacag caggtcagta gccgccgctt attgtcgaat ctggtgagcg aatttcgccg
    61561 cgagttaccg cgccaacagg cacaggaagc gggctacggc cttgccgcgc tgattgacgg
    61621 attatggctg cgcgcggcac tgagcggcaa accgctggat aaacctctcg cacactcgct
    61681 gacccgtcac tttattactc agcatttacc caccgattaa ccgaggagac gtgatgtccc
    61741 gaatggcaga acagcagctt tatatacatg gtggttatac ctccgccacc agcggtcgca
    61801 ccttcgagac cattaacccg gccaacggta acgtgctggc gaccgtgcag gccgccgggc
    61861 gtgaggatgt cgatcgcgcc gtgaagagtg cccaacaggg gcaaaaaatc tgggcggcga
    61921 tgaccgctat ggagcgctcg cgtattctgc gtcgggccgt tgatattctg cgtgaacgca
    61981 atgacgaact cgcaaaactg gaaaccctcg ataccggaaa agcatattcg gaaacctcaa
    62041 ccgtcgatat cgttaccggt gcggacgtgc tggagtacta cgccgggctg atcccggcgc
    62101 tggaaggcag ccagatcccg ttgcgtgaga cgtcatttgt ttatacccgc cgcgaaccgc
    62161 tgggtgtggt ggcggggatt ggcgcatgga actacccgat ccagattgcc ctgtggaaat
    62221 ccgccccggc gctggcggca ggcaacgcaa tgattttcaa accgagcgaa gtcaccccgc
    62281 ttaccgcgtt aaagctggct gaaatttaca gcgaagcggg cctgccggac ggcgtattta
    62341 acgtgttgcc gggggggggc gcggagaccg ggcaatatct gaccgagcat ccgggcattg
    62401 ccaaagtgtc atttaccggc ggtgtcgcca gcggcaaaaa agtgatggct aactcggcgg
    62461 cctcttccct gaaagaagtg accatggaac tgggcggtaa atcaccgctg atcgttttcg
    62521 acgatgcgga tctcgatctc gccgccgata tcgccatgat ggcgaacttc ttcagctccg
    62581 gtcaggtgtg taccaacggc acccgcgtct tcgttccggc gaaatgcaaa gccgcatttg
    62641 agcagaaaat tctggcgcgc gttgagcgca ttcgcgcggg cgacgttttc gatccgcaaa
    62701 ctaacttcag cccgctggtc agcttcccgc atcgcgataa cgtgctgcgc tatatcgcca
    62761 aaggcaaaga ggaaggcgcg cgcgtactgt gcggcggcga tgtactgaaa ggcgatggct
    62821 tcgataacgg cgcatgggtt gcaccgaccg tgttcaccga ttgccgcgac gaaatgacca
    62881 tcgtgcgcga agagatcttc ggtccggtga tgtcgcttct cacttatgaa tctgaggacg
    62941 aagtgattcg ccgcgccaat gataccgact acggcctggc ggcgggcatc gtgacggcgg
    63001 acctgaaccg cgcgcatcgc gtcattcatc agctggaagc gggtatttgc tggatcaaca
    63061 cctggggcga atccccggca gagatgcccg ttggcggcta caaacactcc ggcattggtc
    63121 gcgagaacgg cgtgatgacg ctccagagtt acacccaggt gaagtccatc caggttgaga
    63181 tggctaaatt ccagtccata ttctaaccag gaggtttatt tgcaatttga ctacatcatt
    63241 attggtgccg gctcagccgg caacgttctc gctacccgtc tgactgaaga tccgaatacc
    63301 tccgtgctgc tgcttgaagc gggcggcccg gactatcgct ttgacttccg cacccagatg
    63361 cccgcagcgc tggcgttccc actacagggc aaacgctaca actgggccta tgaaacagag
    63421 cctgaaccgt ttatgaataa ccgccgcatg gagtgcggac gcggtaaagg cctgggtgga
    63481 tcgtcgctga tcaacggcat gtgctacatc cgtggcaatg cgctggatct cgataactgg
    63541 gcgcaagaac ccggtctgga gaactggagt tatctcgact gcctgcccta ctaccgcaag
    63601 gccgagactc gcgatgtggg cgagaacgac taccacggtg gcgatggccc ggtgagcgtc
    63661 actacctcca aacccggcgt caatccgctg tttgaagcga tgattgaagc gggcgtgcag
    63721 gcgggctacc cgcgcacgga cgatctcaac ggttatcagc aggaaggttt tggtccgatg
    63781 gatcgcaccg tcacgccgca gggccgtcgc gccagtaccg cgcgcggtta tctcgatcag
    63841 gccaaatcgc gccctaacct gaccattcgt attcacgcta tgaccgatca catcattttt
    63901 gacggcaaac gcgcggtggg cgtcgaatgg ctggaaggcg acagcaccat cccaacccgc
    63961 gcaacggcca acaaagaagt gctgttatgt gcaggcgcga ttgcctcacc gcagatcctg
    64021 caacgctccg gcgtcggcaa cgctgaactg ctggcggagt ttgatattcc gctggtgcat
    64081 gaattacccg gcgtcggcga aaatcttcag gatcatctgg agatgtatct gcaatatgag
    64141 tgcaaagaac cggtttccct ctaccctgcc ctgcagtggt ggaaccagcc gaaaatcggt
    64201 gcggagtggc tgtttggcgg cactggcgtt ggtgccagca accactttga agcaggtgga
    64261 tttattcgca gccgtgagga atttgcgtgg ccgaatattc agtaccattt cctgccagta
    64321 gcgattaact ataacggctc gaatgcagtg aaagagcacg gtttccagtg ccacgtcggc
    64381 tcaatgcgct cgccaagccg tgggcatgtg cggattaaat cccgcgaccc gcaccagcat
    64441 ccggcgattc tgtttaacta catgtcgcac gagcaggact ggcaggagtt ccgcgacgca
    64501 attcgcatca cccgcgagat catgcatcaa cccgcgctgg atcagtatcg tggccgcgaa
    64561 atcagccccg gcacggaatg tcagacggat gaacagctcg atgagttcgt gcgtaaccac
    64621 gccgaaaccg ccttccatcc gtgcggtacc tgcaaaatgg gttacgacga gatgtccgtg
    64681 gttgacggcg aaggccgcgt acacgggtta gaaggcctgc gtgtggtgga tgcgtcgatt
    64741 atgccgcaga ttatcaccgg gaatttgaac gccacgacaa ttatgattgg cgagaaaata
    64801 gcggatatga ttcgtggaca ggaagcgctg ccgaggagca cggcgggata ttttgtggca
    64861 aatgggatgc cggtgagagc gaaaaaatga gtcgtgatgt gaactaacgc aggaaccgat
    64921 cagattgaga gttaccgttc cagagagggg gaccgaatcc ttatataaac accgaggtaa
    64981 ctctcatgct tcatatccag tatccatgat gctggctttt tgctgatatt taacccgaac
    65041 attcctgata cagactatta atacaacctt atctgcgaca gattttattt tctggatata
    65101 ttatgcgtga acaaatcaaa caggatatcg atctgattga gattttattt tatctgaaga
    65161 aaaaggttcg tgttatcctt tttattatag ctatctgtat ggcaatgatg cttttatttc
    65221 tgtatatcaa taaagataat ataaaagtga gttacagcct aaaaataaac cagacaacac
    65281 caggcatact tgttaactgt gatagcaata ataactttgc ctgtcagacc acaatgactg
    65341 aagatgttat acagcgaatt actacatttt ttcacaccag cccagatgtc aagaacagag
    65401 aaataaagct ggaatggtca ggagataaga gagatttacc aactgctgaa gcggaaatat
    65461 ctcgcgtgca ggcgtctatt atcaaatggt atgcgtcaga ataccataat ggcaggcaag
    65521 ttctcgatga gatacaaaca ccttcagcaa ttaacagtga gctttataca aaaatgatat
    65581 acctgaccag gaactggtca ttgtatccga acggtgatgg ctgtgtaact ataagctcac
    65641 cagaaataaa aaataaatac cctgctgcca tttgcctggc tctgggattt tttctaagca
    65701 ttgtaatttc tgtaatgttt tgccttgtca aaaaaatggt agatgaatac caacaaaact
    65761 ctgggtagta ataaacagca ttctgataga gagtgcctgt ataaagctga agtgacaatg
    65821 accgggaagg gaacccgttt tcaggaatgg atgcctgtct ttttcattgt aaatatcctg
    65881 gcattactaa tgccaggata tttacagaag agcagcataa ccatgacgga acggggttaa
    65941 gtcaggttgt ccttaacgct attttcaacc tcgtatgcct tcttcaggtt tatgttcaga
    66001 tctcatatct ctctcagcaa tcctcaataa tcagatacac cgctttcacc ggaccatgaa
    66061 ccccgacgac tttgataagc tcaatatccg ccgttgaact agggccgcta atgatgttaa
    66121 tgcaggacgg cattcgttca ccggcctgcg ctttctgatg caatttttct gcgagttgcg
    66181 ctacacgcgg caagatagtg cttttacgca ggataaaaag agaagattcc gggagcaggc
    66241 tcaatgaacg cccgcgcccg gcggcggaaa aaagaaccac gcctcccgat tcggttaaac
    66301 catattcagc atacacgaca cccactttag cctgctcggc ctgcgagata ttctcggcac
    66361 ctttcgccgg atcccaaaca acggcattgc attcctgctg caaacgttca ctaatcccca
    66421 attcctccag cctcgtgtca ccgctaatca cgaccgactg atctcccagc tctttacaca
    66481 gacgtattgc agcttctgcc gccttcgcct cgctggtcag ctcacagcgc gtcaacataa
    66541 catcgctggc aaactgaata aacgcgtcac agcgctgctg ttggttaagt tgggtaagcc
    66601 gctcgttagc atagttgtta agcggcgcat cttctgcttg cggttcaagt cgcagcgggc
    66661 gacccagtgc ctgagcaacg ttattcaaaa attcgcctcg attatccatt ctttttctcc
    66721 tgcgcctgat gtttcttaaa ccaactacgg aaactctctc cgtcagcttc aggaagatcg
    66781 cgtgcttcca tccagtcgct aatcgcgcca aatttgagtg gtgttttgcc gccattgata
    66841 aaccagcttg ccgcatgagc gccggccatc atcccgactt tccacaatcc tggatgacta
    66901 ttggcataag cgaacatttt tatcgcccgt tgctctgctt ttgcggtgat acctttttca
    66961 gccatcaccc gacgatgacg caaaatcagt tttgacagcg gaatacgcac cggacacacg
    67021 ctgtcacaag cagtgcataa agagcaggcg tagggtaaat ctttaaaatc tttatagccg
    67081 ccaagtagcg gagaaatcac cgcaccaatt ggccctggat aaatagagcc atatccatga
    67141 ccgccaatat ggcgatatgc cggacaagta ttcatacaag ccccgcagcg aatacagcgc
    67201 agcacatccc gaaattcaga ggccagcacc tcagaacgcc cgttatcgac aataaccaga
    67261 tgaaactctt caggaccatc aacgtgcccc gcttcgcgcg gtcctgtcag ccaggtgttg
    67321 tatcccgtca aacgtgcacc aacggcactg cgcgccagca tggtgatcaa tacatctacc
    67381 tcggcaaacg tgggggcaat acgctccatt cccatcactg caatatgcgt tttaggcagc
    67441 gtggtacaca ttcgcgcatt accttcattg gtcaccaggc ataccgaacc tgtctctgcc
    67501 accgcgaaat tacagccggt aatacctatt tcagcactga ggaaatcttc gcggattttt
    67561 tgccggatga ataaggtcat cgcttcaggc gtttccggcc cctcatagcc cagacgttcg
    67621 tgtagcactc gacggatctg atggcgatct ttatgaattg ccgggaccac aacatgagat
    67681 ggcggatctt gatccagctg gagaatgtat tcacccagat cggtttcaat cacctgaatg
    67741 ccagcatcct gcaacacatg attgacacca atctcttcgg tcaccatcga tttagatttc
    67801 accaccttcc gggcattttt gcgttgggca acctgtaaaa tgtagcgagt ggcgtcttct
    67861 ttagtttttg caaaatagac gtgaccgccg ttttgcgtca ctttttctga gagctggtac
    67921 agataagcgt cgagattact tagaacatga tcacgtatct gggcggcccg atcgcgccac
    67981 tcctcccagt gccccaattc atcgaccatt ttttgccgat ttgccccaat acgctgctgc
    68041 gcgtttgcca ccgctttgcg catgatcgga tcttcaattt gctgacggat gcgtgtctta
    68101 aaatctgtat tactggtttt gatcgacatc tttatatcct cagcggctca tcaacacttc
    68161 agcaatatgc atcactttga ctttctgccc ttcccgttgt aatcgcccac tgatgttcag
    68221 caggcaactc acgtcagcac caattaaata ctcagggcgg acttccatca ggtgcacaac
    68281 cttttctttc accatctcgc cggatatttc ggccattttg accgagaacg tgccgccaaa
    68341 tccgcagcag gtatcctgtt cagcaaaggt caacagctcc agtccacgca catttttcag
    68401 cagcgtaagt ggctcgtcct tcactcccag cttacgggcc aggctacaag atgggtgata
    68461 cacagctctc ccttgcaaac tggcacctac atcgactacc cctaatttat taacaataaa
    68521 agaggtgaga tcctgcatac gcgcggcaac cttttcggca cgtgatgccc attcaggttc
    68581 atccgccaga tacgtcgggt aactttttac ggcataggtg caagagccag ccggtgaaat
    68641 aatgggatcg tcgttatcct ccagtgcggc gatcagattt ttcatccctg gaatcgcttc
    68701 tttgatataa ccgctattga tcgcaggctg accgcagcat ccctgtttct ccgggaaatt
    68761 tacgcgacag ccgagttttt ccagtagcag cacggagtct cgtgccattc ttgatttcag
    68821 ggcgtcacca atacaggtga caaagaaatt gacattcaca actaatactc cattacttca
    68881 tgcccattta tgcccgatgt ttccccccca ccgggggatc aatcatcaat aaatatggcg
    68941 ttctttttta attggctagc aacataccac ccctcatttg tatgacaaat gataatcttt
    69001 catccaaact tcaaatgcaa taactaattt tatttctttc ggcatcgctc acattttatg
    69061 gggaaatcga cgttcatttt tggcataaga aatccattta aaaaggctac atcccttata
    69121 cctattcaga gctacttatt caggggttat tgagatatac actgatggtg tacaaaaaaa
    69181 tcaccgacaa taatcacgta tcaagtaata gtcggggttt tatttaattg aagcaattta
    69241 gtttgcattt ttatttaaaa actgagctag agtccatcag aaaaaaatat ataaagccca
    69301 aaccaccgtt ggaaatagaa atagttgaaa ggctcacaac tcccggaatg cggattatct
    69361 ttgccctgat ttctggcggt tttattcagg gtgcaagctg tctgaccctt tcccgatatt
    69421 ctcccggagt acaaccaaac tcgcggacaa acgccttgtg aaaagatgat tcactggcat
    69481 agcctactga ctcagcgatc accacaacag ggagcatttc ccgggaaaac atctgggccg
    69541 ctatttgtag acgcaacttt gttaatacag ccagcggcgt ggttccggaa acatcacgga
    69601 aaagctgggc aaaacttgcc cgggacatgt gagcgatgct ggccagcgat tcgacggtcc
    69661 aggcgtgtcc tggcatttcc agcatttgct gtattaccgc accaagacgt ggatgcagaa
    69721 gcaaactgag aatgtttttc tcagtattaa cctgtgcaat ccactcacgc accgcgaggg
    69781 taaagaatgt agcgcagatc tggctacaca gtgcatccac tcccggcatc gccgatctgg
    69841 attcctgttg taaaaacggg atcgcctccc tcagccagtt atattccacg ctgtggttaa
    69901 ccggtgctaa aaacagcgtt tccggcaaag acgttaaaaa ataacgcgcc gaatgttgca
    69961 accgaagagt gccgcagaca atacaggtcg attcattatc gacatgactc aggcgatgag
    70021 ctgaattttg tggtagcagg accacatttc ccgggcgtaa tgtaaaaatc tctcccgtcg
    70081 gcatttccag cttcgccgct ccttgcgtta acgcatgcca acgaataacc gataattccc
    70141 cggcaccatg tggaagctgc cagtcacttc ctaacacgca attcttatcg atcgttcctt
    70201 gtggagcgtt aagcatcaac aaacggctaa gggcatccat atcagactcc tgagtggttt
    70261 ttgattacaa acaccaacaa tcttagtaaa taactaatct aataccatca aatggttaat
    70321 catgattttt gttttgtgat tctgattaag aaaaagaaaa taccagacga taagacaaaa
    70381 tatcaagcgt tgtgaagaaa tggtatttgc tcttttgcgt ctatagtcat gatgtcaaat
    70441 gaacgcgttt cgacaggaaa tcatcatgaa taaatatcag gcagtgatta ttggttttgg
    70501 caaggctgga aaaacattag ccgtcacgct ggcaaaagca ggttggcgag tggctctcat
    70561 cgaacaatca aatgcaatgt atggcgggac ctgtattaat atcggctgca tcccaaccaa
    70621 aacattggtt catgacgcac agcagcacac agattttgtc cgtgccatac agcgtaaaaa
    70681 tgaagtggtt aattttttac gtaataagaa ttttcataat cttgcggata tgcccaatat
    70741 cgacgtgatc gacggccagg cggagtttat caataatcat agcctgcgtg ttcatcggcc
    70801 tgaggcaaat ctggaaattc atggcgagaa aatttttatt aataccggtg cacaaaccgt
    70861 ggttccgcca attcctggaa ttaccaccac gccaggagta tatgacagca ccggattact
    70921 taatctaaaa gaattgcctg ggcatttagg tattttgggc ggcggatata ttggcgttga
    70981 gttcgcctct atgttcgcta attttggcag caaggtaacc attttagaag cagcttcgct
    71041 gtttttgcct cgggaagatc gggatattgc tgataatatc gcgacgattt tacgcgatca
    71101 gggcgtcgat attatcctca atgcccatgt ggagcgaatc agtcaccatg aaaatcaagt
    71161 gcaagtgcat agcgagcacg cccaactggc ggtggatgca ctgttaatag cttccggtcg
    71221 tcaaccggct accgcttcgt tacatccaga aaatgccggt atcgcagtaa acgagcgcgg
    71281 ggcaattgtc gttgacaagc aattacatac caccgcagac aatatttggg cgatgggaga
    71341 tgttaccggc gggctgcaat ttacttatat ttcactggat gattaccgca ttgtgcgtga
    71401 tgagttgctg ggagaaggca gacgtagtac tgatgatcgg aaaaatgtgc cttattccgt
    71461 attcatgaca ccgccactgt ccagggttgg tatgacagaa gaacaggcca gagagagtgg
    71521 tgctgatatt caggtggtga cattgcctgt agctgcaatt ccgcgtgcca gagtgatgaa
    71581 tgatactcgt ggggtattaa aagcgattgt tgataataaa acccaacgta tattaggggc
    71641 atcactgctg tgtgttgact cccacgagat gatcaatata gtgaaaatgg tgatggatgc
    71701 cgggctgcct tatagcatat tacgcgatca gatatttact catccgtcga tgagcgaatc
    71761 actcaatgat ctattttcat tagtcaaata aactcaaaat cagacgccag aacaaatatt
    71821 ctggcgtctc agagaaaaga atctta
//
LOCUS       DAZGJW010000006        66250 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZGJW010000006 DAZGJW010000000
VERSION     DAZGJW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.006"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            27..530
                     /locus_tag="ACHKIK_000417"
     CDS             27..530
                     /locus_tag="ACHKIK_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO9081655.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(597..2054)
                     /gene="nuoN"
                     /locus_tag="ACHKIK_000418"
     CDS             complement(597..2054)
                     /gene="nuoN"
                     /locus_tag="ACHKIK_000418"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO9081656.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2061..3590)
                     /gene="nuoM"
                     /locus_tag="ACHKIK_000419"
     CDS             complement(2061..3590)
                     /gene="nuoM"
                     /locus_tag="ACHKIK_000419"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO9081657.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFDISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSRSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3754..5595)
                     /gene="nuoL"
                     /locus_tag="ACHKIK_000420"
     CDS             complement(3754..5595)
                     /gene="nuoL"
                     /locus_tag="ACHKIK_000420"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO9081658.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLVT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5592..5894)
                     /gene="nuoK"
                     /locus_tag="ACHKIK_000421"
     CDS             complement(5592..5894)
                     /gene="nuoK"
                     /locus_tag="ACHKIK_000421"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO9081659.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5891..6445)
                     /gene="nuoJ"
                     /locus_tag="ACHKIK_000422"
     CDS             complement(5891..6445)
                     /gene="nuoJ"
                     /locus_tag="ACHKIK_000422"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO9081660.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6457..6999)
                     /gene="nuoI"
                     /locus_tag="ACHKIK_000423"
     CDS             complement(6457..6999)
                     /gene="nuoI"
                     /locus_tag="ACHKIK_000423"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO9081661.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7014..7991)
                     /gene="nuoH"
                     /locus_tag="ACHKIK_000424"
     CDS             complement(7014..7991)
                     /gene="nuoH"
                     /locus_tag="ACHKIK_000424"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO9081662.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7988..10714)
                     /gene="nuoG"
                     /locus_tag="ACHKIK_000425"
     CDS             complement(7988..10714)
                     /gene="nuoG"
                     /locus_tag="ACHKIK_000425"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO9081663.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGA
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10767..12104)
                     /gene="nuoF"
                     /locus_tag="ACHKIK_000426"
     CDS             complement(10767..12104)
                     /gene="nuoF"
                     /locus_tag="ACHKIK_000426"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO9081664.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12101..12601)
                     /gene="nuoE"
                     /locus_tag="ACHKIK_000427"
     CDS             complement(12101..12601)
                     /gene="nuoE"
                     /locus_tag="ACHKIK_000427"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO9081665.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12604..14406)
                     /gene="nuoC"
                     /locus_tag="ACHKIK_000428"
     CDS             complement(12604..14406)
                     /gene="nuoC"
                     /locus_tag="ACHKIK_000428"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO9081666.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14500..15162)
                     /gene="nuoB"
                     /locus_tag="ACHKIK_000429"
     CDS             complement(14500..15162)
                     /gene="nuoB"
                     /locus_tag="ACHKIK_000429"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO9081667.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15178..15621)
                     /gene="nuoA"
                     /locus_tag="ACHKIK_000430"
     CDS             complement(15178..15621)
                     /gene="nuoA"
                     /locus_tag="ACHKIK_000430"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO9081668.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16252..17190)
                     /gene="lrhA"
                     /locus_tag="ACHKIK_000431"
     CDS             complement(16252..17190)
                     /gene="lrhA"
                     /locus_tag="ACHKIK_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO9081669.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18110..19327
                     /gene="alaA"
                     /locus_tag="ACHKIK_000432"
     CDS             18110..19327
                     /gene="alaA"
                     /locus_tag="ACHKIK_000432"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO9081670.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19411..20010
                     /gene="yfbR"
                     /locus_tag="ACHKIK_000433"
     CDS             19411..20010
                     /gene="yfbR"
                     /locus_tag="ACHKIK_000433"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO9081671.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20069..21901)
                     /locus_tag="ACHKIK_000434"
     CDS             complement(20069..21901)
                     /locus_tag="ACHKIK_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO9081672.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(21988..22638)
                     /gene="hxpA"
                     /locus_tag="ACHKIK_000435"
     CDS             complement(21988..22638)
                     /gene="hxpA"
                     /locus_tag="ACHKIK_000435"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO9081673.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(22649..23143)
                     /locus_tag="ACHKIK_000436"
     CDS             complement(22649..23143)
                     /locus_tag="ACHKIK_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO9081674.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23226..23681)
                     /gene="yfbV"
                     /locus_tag="ACHKIK_000437"
     CDS             complement(23226..23681)
                     /gene="yfbV"
                     /locus_tag="ACHKIK_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO9081675.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24019..25221
                     /gene="ackA"
                     /locus_tag="ACHKIK_000438"
     CDS             24019..25221
                     /gene="ackA"
                     /locus_tag="ACHKIK_000438"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO9081676.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25296..27440
                     /gene="pta"
                     /locus_tag="ACHKIK_000439"
     CDS             25296..27440
                     /gene="pta"
                     /locus_tag="ACHKIK_000439"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO9081677.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27630..29150
                     /gene="yfcC"
                     /locus_tag="ACHKIK_000440"
     CDS             27630..29150
                     /gene="yfcC"
                     /locus_tag="ACHKIK_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO9081678.1"
                     /translation="MSATTESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29183..29725)
                     /gene="yfcD"
                     /locus_tag="ACHKIK_000441"
     CDS             complement(29183..29725)
                     /gene="yfcD"
                     /locus_tag="ACHKIK_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO9081679.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29783..30334)
                     /gene="yfcE"
                     /locus_tag="ACHKIK_000442"
     CDS             complement(29783..30334)
                     /gene="yfcE"
                     /locus_tag="ACHKIK_000442"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO9081680.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30390..31034)
                     /gene="yfcF"
                     /locus_tag="ACHKIK_000443"
     CDS             complement(30390..31034)
                     /gene="yfcF"
                     /locus_tag="ACHKIK_000443"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO9081681.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31170..31817
                     /gene="yfcG"
                     /locus_tag="ACHKIK_000444"
     CDS             31170..31817
                     /gene="yfcG"
                     /locus_tag="ACHKIK_000444"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO9081682.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            31874..32236
                     /gene="folX"
                     /locus_tag="ACHKIK_000445"
     CDS             31874..32236
                     /gene="folX"
                     /locus_tag="ACHKIK_000445"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO9081683.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32257..33150
                     /locus_tag="ACHKIK_000446"
     CDS             32257..33150
                     /locus_tag="ACHKIK_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO9081684.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33198..34088)
                     /gene="rpnB"
                     /locus_tag="ACHKIK_000447"
     CDS             complement(33198..34088)
                     /gene="rpnB"
                     /locus_tag="ACHKIK_000447"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO9081685.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(34285..35058)
                     /gene="hisP"
                     /locus_tag="ACHKIK_000448"
     CDS             complement(34285..35058)
                     /gene="hisP"
                     /locus_tag="ACHKIK_000448"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO9081686.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35066..35782)
                     /locus_tag="ACHKIK_000449"
     CDS             complement(35066..35782)
                     /locus_tag="ACHKIK_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9081687.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(35779..36465)
                     /gene="hisQ"
                     /locus_tag="ACHKIK_000450"
     CDS             complement(35779..36465)
                     /gene="hisQ"
                     /locus_tag="ACHKIK_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO9081688.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(36555..37337)
                     /gene="hisJ"
                     /locus_tag="ACHKIK_000451"
     CDS             complement(36555..37337)
                     /gene="hisJ"
                     /locus_tag="ACHKIK_000451"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO9081689.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(37558..38340)
                     /gene="argT"
                     /locus_tag="ACHKIK_000452"
     CDS             complement(37558..38340)
                     /gene="argT"
                     /locus_tag="ACHKIK_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO9081690.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(38606..39175)
                     /gene="ubiX"
                     /locus_tag="ACHKIK_000453"
     CDS             complement(38606..39175)
                     /gene="ubiX"
                     /locus_tag="ACHKIK_000453"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO9081691.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39270..40787)
                     /gene="purF"
                     /locus_tag="ACHKIK_000454"
     CDS             complement(39270..40787)
                     /gene="purF"
                     /locus_tag="ACHKIK_000454"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO9081692.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(40824..41312)
                     /gene="cvpA"
                     /locus_tag="ACHKIK_000455"
     CDS             complement(40824..41312)
                     /gene="cvpA"
                     /locus_tag="ACHKIK_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO9081693.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(41571..42233)
                     /gene="dedD"
                     /locus_tag="ACHKIK_000456"
     CDS             complement(41571..42233)
                     /gene="dedD"
                     /locus_tag="ACHKIK_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO9081694.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKSKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42223..43491)
                     /gene="folC"
                     /locus_tag="ACHKIK_000457"
     CDS             complement(42223..43491)
                     /gene="folC"
                     /locus_tag="ACHKIK_000457"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO9081695.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(43561..44475)
                     /gene="accD"
                     /locus_tag="ACHKIK_000458"
     CDS             complement(43561..44475)
                     /gene="accD"
                     /locus_tag="ACHKIK_000458"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO9081696.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPLGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(44631..45290)
                     /locus_tag="ACHKIK_000459"
     CDS             complement(44631..45290)
                     /locus_tag="ACHKIK_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO9081697.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(45373..46185)
                     /gene="truA"
                     /locus_tag="ACHKIK_000460"
     CDS             complement(45373..46185)
                     /gene="truA"
                     /locus_tag="ACHKIK_000460"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO9081698.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVMRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTRAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46185..47198)
                     /locus_tag="ACHKIK_000461"
     CDS             complement(46185..47198)
                     /locus_tag="ACHKIK_000461"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO9081699.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47264..48400)
                     /gene="pdxB"
                     /locus_tag="ACHKIK_000462"
     CDS             complement(47264..48400)
                     /gene="pdxB"
                     /locus_tag="ACHKIK_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO9081700.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            48499..49494
                     /gene="flk"
                     /locus_tag="ACHKIK_000463"
     CDS             48499..49494
                     /gene="flk"
                     /locus_tag="ACHKIK_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO9081701.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(49491..50669)
                     /locus_tag="ACHKIK_000464"
     CDS             complement(49491..50669)
                     /locus_tag="ACHKIK_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9081702.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(50953..52173)
                     /gene="fabB"
                     /locus_tag="ACHKIK_000465"
     CDS             complement(50953..52173)
                     /gene="fabB"
                     /locus_tag="ACHKIK_000465"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO9081703.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            52332..54338
                     /gene="mnmC"
                     /locus_tag="ACHKIK_000466"
     CDS             52332..54338
                     /gene="mnmC"
                     /locus_tag="ACHKIK_000466"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO9081704.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFNQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(54459..54737)
                     /locus_tag="ACHKIK_000467"
     CDS             complement(54459..54737)
                     /locus_tag="ACHKIK_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO9081705.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(54771..55319)
                     /gene="epmC"
                     /locus_tag="ACHKIK_000468"
     CDS             complement(54771..55319)
                     /gene="epmC"
                     /locus_tag="ACHKIK_000468"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO9081706.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(55319..56128)
                     /locus_tag="ACHKIK_000469"
     CDS             complement(55319..56128)
                     /locus_tag="ACHKIK_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO9081707.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLFDSHGQEILHWLGMN"
     gene            complement(56128..56952)
                     /gene="mepA"
                     /locus_tag="ACHKIK_000470"
     CDS             complement(56128..56952)
                     /gene="mepA"
                     /locus_tag="ACHKIK_000470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO9081708.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSSLWKPEI
                     FSLIKLAAQDKNVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(56956..58041)
                     /gene="aroC"
                     /locus_tag="ACHKIK_000471"
     CDS             complement(56956..58041)
                     /gene="aroC"
                     /locus_tag="ACHKIK_000471"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO9081709.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58076..59008)
                     /gene="prmB"
                     /locus_tag="ACHKIK_000472"
     CDS             complement(58076..59008)
                     /gene="prmB"
                     /locus_tag="ACHKIK_000472"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO9081710.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            59174..59725
                     /gene="smrB"
                     /locus_tag="ACHKIK_000473"
     CDS             59174..59725
                     /gene="smrB"
                     /locus_tag="ACHKIK_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO9081711.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(59798..60649)
                     /locus_tag="ACHKIK_000474"
     CDS             complement(59798..60649)
                     /locus_tag="ACHKIK_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO9081712.1"
                     /translation="MHKIRHVICLLVLALCASGIQAATKIATLYVPAGTTSVVAKYRF
                     HLSVLTPQSVEYGTYESNSTAAASLPLVSWTGSPPGPELRMERNNTTLPDSTCPGLEE
                     YDALSPVTAWSCNELVLGVYYDGDLHGCPWIVSSYVESASTMDQRFGAGIFIGPTKVS
                     SDCPTESLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMENGKLCDGSNFDER
                     GAYCRFVAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDTTSRQPIDSTCRF
                     TYILNMY"
     gene            complement(60651..61190)
                     /locus_tag="ACHKIK_000475"
     CDS             complement(60651..61190)
                     /locus_tag="ACHKIK_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9081713.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGARAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVAGQSAHLPVVFKLKDCKGPAGYNVKVTLTGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
     gene            complement(61187..61675)
                     /locus_tag="ACHKIK_000476"
     CDS             complement(61187..61675)
                     /locus_tag="ACHKIK_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9081714.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTNFSAYATLRVD
                     YQ"
     gene            complement(61672..62181)
                     /locus_tag="ACHKIK_000477"
     CDS             complement(61672..62181)
                     /locus_tag="ACHKIK_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9081715.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(62197..62949)
                     /locus_tag="ACHKIK_000478"
     CDS             complement(62197..62949)
                     /locus_tag="ACHKIK_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO9081716.1"
                     /translation="MFNLTNTAKIVVPALALLATVVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFICTGNSCQVDEEQSRKG"
     gene            complement(62969..65614)
                     /gene="yfcU"
                     /locus_tag="ACHKIK_000479"
     CDS             complement(62969..65614)
                     /gene="yfcU"
                     /locus_tag="ACHKIK_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFO9081717.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHIEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDVGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGY
                     RYEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPA
                     VKPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(65696..>66250)
                     /locus_tag="ACHKIK_000480"
     CDS             complement(65696..>66250)
                     /locus_tag="ACHKIK_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9081718.1"
                     /translation="FVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCSIVP
                     DDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSANSGN
                     YYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNFKAW
                     LVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16272 a        18109 c        16717 g        15152 t
ORIGIN      
        1 catgaaattc aatggaggaa aaataaatgg gaatgattgg ttattttgca gaaattgact
       61 cagaaaagat taaccaactg cttgagtcta ctgaaaaacc tttgatggat aatatacatg
      121 acacgctttc aggattacgc cgactggata ttgataaacg ctgggatttt ttacactttg
      181 ggctgacagg cacctctgct tttgatcccg cgaagaatga tccgctgagt cgtgccgtgc
      241 ttggtgaaca cagcctcgaa gatggcatag atggcttcct gggattgacc tggaatcagg
      301 agttagccgc gacaatcgac cgactggaat cactggatcg aagtgagctg cgtaaacagt
      361 tttccattaa gcgactcaat gaaatggaga tttatccggg agtgaccttt tcagaagagt
      421 tagaggggca actctttgct tcgatcatgc ttgatatgga aaagctgatt agcgcgtatc
      481 gacgtatgct gcgacaaggg aatcatgcgc tgacggttat tgttgggtga tggtgtcagg
      541 gcaccaaaaa aaaccgccga acatgtcggc ggctttctga cttacaaact aacagattac
      601 atcagcggca ttgctaaccg cacaatgcta atcagcggtt gtggccatac acccagcacc
      661 agtaccaaca gtgcagaaat cagcaccacg ataccgcccg cgctgtactg ccagtttgat
      721 ggtgcatcgc gacccggttg ttccggggcg tgaagataca ggctcaccgc cacgcgcagg
      781 tagtagtaga ggccgattgc cgaaccgaca accacggcac ccaccagcca ccacaagtgt
      841 gcctggacac cgaccgccag cacgtagaac ttaccgataa agcccagcgt catcgggata
      901 ccggccagag acagcatcat caccgtcatc actgccgcga ggatcggacg atgccagaac
      961 agaccgcggt aagagaacag ggaatcagca tccgggccac gatacgggct ggacatcagg
     1021 ctgaccacgc cgaacgcgcc gaggctgctg aacagataac cggccaggta aacccctacc
     1081 gcttccatcg acatctcacc agtttgcagc gcaatcagcg ccaccagcag atagccgagg
     1141 tgagagatag atgagtaacc gagcagacgt ttgatgttgg tctggctcag cgccatcagg
     1201 ttaccgaaga tgatggaggc aaaggcgata atcgccagta ctacgcgaat cgcttcgctg
     1261 tcacccaccg gtgcgtagag gaacagacgc atcaccacac cgaagatagc gattttgctc
     1321 gccgtcgcca ggaaagtgga aaccggcgca ggcgcgccct ggtatacgtc tggcgtccac
     1381 aggtggaacg gcaccagcga gagtttgaag ccgaggccaa caatcatcag tccgaaacct
     1441 gccagcaaca gcggctcgtt gagcataccg tcgccgaggt ttttacccag cgcgacaaac
     1501 gacaggtcgc cagactgtgc atacaccagc gccataccaa acagcaggaa agaagacgct
     1561 gcggcagaaa ggatggtgta tttgatactg gcttccagcg aacgtttctg gcggaaagcg
     1621 taaccgacca ggccaaacag cggcaaagag atcagttcaa taccgaggaa caaagacgcc
     1681 agatggttgg cattcgccag caggatcccg cccagcgcgg caattaacac caacaggtag
     1741 aactcatcct tgttgtcgtt atagccttca agccacgggt aggcgaaagt acaggtggcg
     1801 aggctcgcca acaataccag cccggtgtaa agcatggcga aaccatcaac gcgcatcagc
     1861 ggcgtaacgt ccatagcgcc cgcctggccg acaaaccaga gcgaaaccag cgccgcgtta
     1921 agcccaataa ccgagagcgt agcgttgagg aaatgattgc gtcgccacgc aatggagagc
     1981 atcacaacca ccaccgtcaa gccgacgatc agcaacggta gcagtgcgat caggttttgt
     2041 ggagttattg tcatggcgat ttacggcctt gtagtagtaa cggaattaac aaaccactgc
     2101 tggatattgc caatcgcgga gcgcgaggta tccagaatcg gctgcggata gaagcccagc
     2161 agtaccagca gcaccaccag caacaggatc ataaacagct cacgcagcga catccctggc
     2221 agttcctggc tggcaatctg gcttttcgct ttaccgaagt aagcgcgatg caacatcgcc
     2281 agcgaataaa cagatgcaaa gaccagccca aaggtagaga taacggtaat caccgggaca
     2341 acctggaagc tgccgaacag aatcataaat tcgccgacga agttaccggt gccaggcatc
     2401 ccaagcgttg ccaccgcaaa gaacagcgac agtgctggca gccatttcat cttgctccac
     2461 agaccgccca tcatgcgcat gtcgcgggta tggatacgtt cataaagctg accacaaaga
     2521 ataaacagac ccgccgccga caagccgtgc gcaatcatct ggattaccgc gccctggtag
     2581 gccaactggc tgccggtgta gatagcaatc agcacgaagc ccatgtggga aaccgaggtg
     2641 taggcgatca gacgtttgat atcggtctgg gcgaaggcca tccacgcacc gtagaagatg
     2701 ccgataacac ccagccacat agcaattggc gcgaactctg ccgacgcgtt cgggaacagc
     2761 ggcagggaga aacgcagcaa accgtaagcg gcagttttca gcaagatccc cgcgaggtca
     2821 acggaaccgg cggtcggagc ctgggagtgc gcatccggca gccagccatg cagcggaacc
     2881 accggcattt tgactgcgaa ggcgatgaag aagcccagca ttaacaggta ttccacacca
     2941 ctggacattg gcgtattcag cagctcttca tagttgaagg tccagacgcc ggtcgcattg
     3001 tagtgaacaa aaaccagcgc caggatggca atcaacatca ccaggccact cgcctgggtg
     3061 taaatgaaga acttggttgc cgccgtgata cgcgttttac cgtcagaggc tttatgcccc
     3121 cacagtgcga tcaggaagta catcggcacc agcatcattt cccagaagaa gaagaacagg
     3181 aacatgtcga tggcaaggaa cacgccgata acgccgccca ggatccacat caggttgagg
     3241 tggaagaagc cctgatattt ttcgatctct ttccacgaac atagtaccgc cagcacaccg
     3301 agcagaccgg tcagcacgac catcagcagc gacagcccgt caatggcgag atgaatagag
     3361 atatcaaaac gcgggatcca cggcatgtcg aattcagact gccactgcgg aattccggcg
     3421 gattgcgtca gtgaataacc gccctgcaac cacagttgca gcgacagcgc cagcgtcaat
     3481 cccatggtga tcagcgcgat ccagcgtggc accttgacgc caaagcgttc ggtctgccag
     3541 cacaggaagc cgccaataaa gggaattaat attagccagg gtagtaacat ggcgatcttt
     3601 attccttgta aaagtcccgt caggaccgga ttttcaacga attctcacga cagaaaattc
     3661 tcttcggact gggggtagtg tcggatgcac cgcgaaggcg gcttatccga cctacgttct
     3721 tatatgccca ggggcaatcc cacaatcctt aactcaacgc agtaccatca acagtgccag
     3781 caccacgacc gcaccgatgc tcatggatgc cacataccag cgcagatagc cgttctcact
     3841 taacagcaga cctttacctg caaagcggga aaggacagcc gggatgttca tcattgagtt
     3901 cagcggatcg cgtttcagca accaggcaat acccaggaac ggcttgacga acactttgtc
     3961 atacagccag tcaaatcccc aggcgttgta ccaccaggtg cccagcagac ggcccggcgc
     4021 actgttggcg atggaggtca ccagagtacg tttacccagc cacagccagg ctgccagcag
     4081 aatgccgacc accgcgacca cgccagaggt aatttccagg gtcaacatgc tgccgtgcgc
     4141 cagttccgtc gtttgcggaa gcacgccctg cagcggcggt acaatcagtg cgccaacgaa
     4201 ggtggaaagg atcagcagca caatcagcgg caggctgtga gttacccctt tcacggcgtg
     4261 agcgtgaatt tgttcttttc cgtggaagac gatgaaaatc atacggaagg tgtagagcga
     4321 ggtcataaac gcaccgacca gacctgccac catcagattg atatgaccat tcgccatcgc
     4381 acccgcgagg atctcatcct tactgaagaa gcccgcagtg accagcggta gtgccgacag
     4441 tgctgcgccg cccaccagga agcagagata aaccagcgga atagatttac gcagaccgcc
     4501 catcttgaag atgttctgtt cgtgatggca ggccagaatg acggaaccgg atgccaggaa
     4561 cagcagcgct ttaaagaacg cgtgagtcat caagtggaaa atcgccgcat cccatgcctg
     4621 cacgccaagc gcgaggaaca tgtagccaat ctggctcatg gtagagtaag cgagaacacg
     4681 tttgatgtcg gtctgtacca gcgcggcaaa accggccagc agcagcgtaa ccgccccgac
     4741 aatacccacc agatgcagaa cttccggcgt caccaggaac aggccgtggg tacgggcgat
     4801 caggtagaca cccgcggtta ccatcgttgc ggcgtggatc agcgcggaga caggcgtcgg
     4861 gcccgccatc gcgtcagcaa gccatgtctg caacggcaac tgcgcagatt taccgaccgc
     4921 accgcccagc agcatcagcg tcgcccacat cagcatgtta ttgccgtcag caaagtgcgc
     4981 tggtgccagt tccaccattt cgcggaagtt cagggtgccc agttcgttgt aaagaatgaa
     5041 cagtgcgaaa gcgaggaaaa cgtcacccac acgggtcacg acgaacgctt tcattgccgc
     5101 tgcgccattc ttcggatcgg tgtaatagaa accgatcagc agataggagc acaggcccac
     5161 gccttcccag ccgaggtaca tcagcagcag gttatcggcg agaaccagaa ctaccatgct
     5221 ggcgatgaac aggttggtgt aggcgaagaa gcgagagtag ccctcttcac cgcgcatgta
     5281 ccaggaggcg tacatgtgaa taaggaaacc cacaccagtg accaccgaga gcatggtcag
     5341 cgacaggccg tccagcacca ggttaaaacc gatgttaaag tcgcctaccg acatccacgt
     5401 ccacagcggc tggctgtatg actgctcgcc gttagcgaag aaatcaacgc cgataaaggc
     5461 ggttaccagc gccgccaggc ccacagagcc aacgccgacg atcgccgaaa cgttttcaga
     5521 ccagcgccca cgggagaacg ccagcaggac gaagccaatc aatggcaaaa taatggttaa
     5581 ggcaagcatg ttcatccgcg catctcactt actgaatcga tgttcaggtt ctggcgacga
     5641 cggtgaagtt gcagcagcag cgcaaggccg atactcgctt ctgccgccgc gaggctgatg
     5701 gcgagaatgt acatcacctg accgtcggtc tgaccccagt agcttccggc gaccacaaag
     5761 gccagcgcgg aggcgttaat catgatttcc agaccaatca gcataaacag cagattgcga
     5821 cggataacca gaccggttaa gccaagaacg aataagattg ccgcgaggat cagtccatgt
     5881 tgtaagggga tcatgcgtgc tcctccgttt ttcttttcgc gctgtcgtct ttacgattgc
     5941 tcagcacttc acccgcacgc tcttcacgac cgacgtggaa ggccacaacc agacccgcga
     6001 gcagcagcat agaagccagt tccaccgcca gtacgtaagg cccgaacagc gtaataccca
     6061 ctgctttagc actgattggc gtaccgtcga taccctgatc attaacacca aggatggcgt
     6121 aaacaatcac caccagcatg atggccgaca aaattgccgg accaatccac acctgcggtt
     6181 tcagccactg gcgttcctgt tcgatttctg aaccgcccag gttgagcatc atcaccacga
     6241 acacgaacag caccataatg gcacccgcgt agacgataat ttccagcgca ccggcgaagt
     6301 aagcgcccag tgagaagaac accccggaaa tcgccagcag cgaaataatc aggtacagca
     6361 gtgcgtgtac cggattggta tgggtgatca ctcgcaaggt cgcaagtatg gctatcaggc
     6421 cacagatata aaaagcgaac tccattgccc ctctccttac ggtaacaggc tcttgacgtc
     6481 gataggcttg gcttcgttct ctgcttcgcc cttatctttg ccgtcgattg ccatacctgc
     6541 catccggtag aagttatatt ccgggtattt gcccggaccg gagatcagca gatcctcttt
     6601 ttcgtaaacc agatcctggc gcttgtattc ccccatttcg aaatccgggg ttaactgaat
     6661 cgccgtggtc ggacaggctt cttcgcacag accacagaaa atgcagcgtg agaagttgat
     6721 gcggaaaaat tccgggtacc agcgaccgtc tttggtttct gctttttgca gcgagataca
     6781 gccgaccggg caggctaccg cgcagaggtt acaggctacg caacgctctt cgccgtccgg
     6841 gtcgcgggtc agaacgatac gaccacgata acggggcggc agatagaccg gctcttccgg
     6901 gtacattcgc gtttcgcgtt tggcgaacgc gtgcaggccg atcatccaga tactacgaac
     6961 ctgggtgccg aaacctacta acaattcttt taaggtcatg gtcttattgc cccttattgc
     7021 gcctgccaga gaatgacagc cgccgttacc agcaagttga tcagcgtcag cggcaggcag
     7081 attttccagc cgaaggacat tacctggtca taacgcggac gcggtaacga cgcacgaatc
     7141 aaaatgaaca tcatcataaa gaacgcggtt ttcagcgcga accagatgaa tggcggtaac
     7201 aacgggcctt gccagccacc gaagaacagc gtcaccatca atgcagagat ggtcacaatc
     7261 ccgatgtact cacccacgaa gaacagaccg aacttcatac cggaatattc aatgtggtaa
     7321 ccatccgcca gttcctgctc ggcttccggc tggtcaaacg ggtgacggtg acataccgcc
     7381 acgcccgcga tggcaaaggt aataaaacca aagaattgcg ggataacgtt ccacacatgc
     7441 gcctggctgt tgacgatgtc ggtcatgttg aatgaaccgg cctgcgccac cacgcccatc
     7501 agggaaagcc cgaggaacac ttcgtagctc agggtctgcg cagaagcacg catcgcaccc
     7561 agcaacgagt atttgttgtt acttgaccag cccgcaaaca gcaccgcgta aaccgccaga
     7621 cctgccatca tcaggaagaa caaaatcccg atgttcaggt cggcaaccac ccaacccgga
     7681 ctgactggca caatcgcaaa ggccagcagc agcgaggtaa aggcaatcat cggtgccagg
     7741 gtaaagatga cgcgatccga gaatttcggg atccagtctt ctttaaagaa cattttgatc
     7801 atgtccgcaa ccagctggag cgaaccgccc cagccaacac ggttaggtcc gtaacggttc
     7861 tggaacagac ccagcaggcg acgttcgcca aagctcatga atgccccgca ggtgacaacc
     7921 accagcagga tcaccaccgc tttgaggatg gtcagcagga tctcaatcag ttccggtgat
     7981 atccaactca ttgttgtgcc tccttgagat cctcaagatg cgcgccagcc agcaccggag
     8041 caatgccgga catacccatc ggcaaaccca cctgccctgc cgtcagccct tcagcgattt
     8101 caaccggcag cgtgaccgtg ttgccatcgt aactaaagga gacgcgagcg cctgcgttca
     8161 cgcccaactt cgcggcatcc gctgggttga gtttgatgta cggctgcggc atacggctct
     8221 ggaagaccgg agcacgctgt gacaattcat cgctgccaaa caggtggtaa tacggcgcga
     8281 tacgccattt cccgtcctgc ggctggaagc gtgccggtac gctggtgaag taatccagac
     8341 cattttcgct ggtttcaaac agacgcacgc ccggatcgcc aaagcgcagt ttgccgccca
     8401 cttcgtcctg gaatttgttc cacgcctgcg gggagttcca gcccggcgcc caggcaaacg
     8461 gcacttgcga acggtgcgca gtcggctggt tgttaccttc catcgagaag gtgaacatgg
     8521 tgtcaatatc ctgcggctga cgcggctcat gaacgctgat attggcgcgc atggcggtac
     8581 gaccgctgta acggtgcggt tcacgggcca gtttctgccc acgaatacgg aatgtcgcat
     8641 ccggcgcagc atctttgata cctgccagtt ccgggatttt cgccacaaca gcgtcaatca
     8701 catggtcgag ctgcgtccag tccacttcac ggctcagcag ggtgctgtgc agcgagtgta
     8761 accagcgcca gctttccagc atgacagttt tgctgtcgta ataagcagga tcgtaaacct
     8821 ggaagaaacg ttgggcgcgg ccttcgttgt tgatcaccgt accgtcgctt tcagcaaagc
     8881 tggcagcaga aagtaccaga tgggcgtttt ccataatcgc tgtgcgttga tgatcaacca
     8941 ccatcaccag cggcgcttta gccagcgcag cattcacgcg ggtagcggaa gcgtgacgat
     9001 gcagatcgtt ttccagcacc accaccgcgt cggcgcgtcc ggtttccagt tcggtcaacg
     9061 cttcttcaag cgaaccgcca cccataatgc ccagccccat gctgttgacg gaacgggcaa
     9121 tcatggtgat accaacgtca gcgccgcgac ctttcagggc tttcgcgacg ttagccgcag
     9181 cctgaatcac ctctatgcta ccggcgttcg tcccggagat aatcaacggt ttcttcgcac
     9241 ctgccagtgc ctgcacgatg acgtcgattt tgctttgcag ctcaggttcg ataccgtcaa
     9301 ccgctggcgc agagttatcc agcgcatggg cgatggcaaa acctaaacgc gcctgatctt
     9361 caaccggtgc gcggtaagtc cacgccgcga tatcatccag acgggtgtca tcaacgttgg
     9421 taacaaacag cggatgcttc gcacgttgac cgatgttgag gattgccgca atctgccagt
     9481 cagccacttt ctgtgctgcc gccatttcgc gcgctttacc tttcacagcc tgacgcactg
     9541 ccagcgcgac gcgcgcgccg gtctgggtaa cgtcttcgcc cagcaccagt accgcatcgt
     9601 aagattcgat ttcgcgcaga gccggagtat aaatgccgcc ttcacgcagc actttcagcg
     9661 ccagttgcag acgttcctgc tcaccgtgag cgataccggt gtagaagttt tcttcgccca
     9721 ccagttcacg cagcgcaaag ttgctttcca cgctggcacg cggagaacca ataccgatca
     9781 ctttcttcga ctgacgcaga atatctgccg cgccctgcat tgcctgttcg gcgttgaggg
     9841 taatgaaatc atcgccacga cgctgtactg gctgacgcgg acgatccttc aggttgacgt
     9901 aaccgtaacc gaaacgacca cggtcgcaga ggaagtagtg gtttaccgta ccgttgtaac
     9961 ggttttcgat acgacgcagt tcgccgtaac gttcaccggg gctgatgtta cagccgatgg
    10021 aacattgctg gcagatgctc ggcgcaaact gcatatccca tttacggttg taacgctcgg
    10081 agtgcgtttt gtcggtaaat acgccggtcg ggcaaatttc gaccaggtta ccggaaaatt
    10141 cgctttccag cgtgccgtct tccgggcgac cgaagtagac gttgtcgtgc gcaccgtaaa
    10201 cgcccagatc tgtaccgtca gcgtaatctt tgtagtaacg cacacagcgg tagcaggcga
    10261 tgcagcggtt catttcgtga gagatgaatg gccccaaatc ctgattacgg tgggtacgtt
    10321 tggtgaaacg gtagcgacgg aagctgtgtc cggtcatcac ggtcatatcc tgaagatggc
    10381 agttaccgcc ttcttcacat accggacagt cgtgcgggtg gttggtcatt aaccactcga
    10441 ccacactttc acggaactgt ttcgcttctt cgtcgtcaat ggaaataaag gtgccatcgg
    10501 aagccggtgt catacaggac atcaccaggc gaccacgcgt gtcttccgcg ttttggtatt
    10561 gcttcaccgc acactggcgg caagcaccga cgcttcccag cgccggatgc cagcaaaagt
    10621 aaggaatatc aaggcccaga gacagacaag cttccagcag gttgtccgct ccgttgacct
    10681 cgtattcttt gccgtctaca tgaattgtag ccattagcat gcttccagtt ttctcagtca
    10741 gagactgagc gttaatcgaa attcggttac cagcgctctt tcagcaggtt cggctgaatc
    10801 ccattaatca aatgggtatt gctgaacggc tgtttgattc ccgcctcaaa ttcttcgcgg
    10861 aaatatttga tggcgctctg taacggctcc actgcaccag gtgcgtgggc acagaaagtt
    10921 ttacccgggc ctaagaatcg acacagttgc tcaagtgttt cgatatcgcc cggctgacct
    10981 tcaccacgct ccagcgcacg cagaattttc acgctccacg gcagaccgtc gcggcacggc
    11041 gtacaccagc cgcaggattc acgggcgaaa aactcttcca ggttacgcac cagcgacacc
    11101 atgttgatct catggtcaac cgccatcgcc agcgccgtac ccagacggct tcctgcttta
    11161 ccgatacttt cgaattccat cggcaggtca agatgcgctt cggtcaggaa gtcggtgccc
    11221 gcgccgcctg gctgccaggc tttgaatttc agaccgtcac gcataccacc ggcgtaatct
    11281 tcgaggatct cgcgcgcggt agtaccaaac ggcagttccc acaggcccgg atttttcacc
    11341 cgaccggaga agcccatcag cttggtgcca gcatctttac ttttcgagat gttctgatac
    11401 cactccacgc cgttagcgag gatcgccgga acgttacaca gggtttcgac gttgttgaca
    11461 caggtcggtt taccccatgc gccggaggtc gccgggaatg gaggcttcga gcgtgggtta
    11521 gcacgacgtc cttccaggga gttgattaac gctgtttctt ccccgcagat gtagcgccct
    11581 gccccggtat ggacgaacag ttcgaagtca aaacctgttc ccataatatt tttgccaagc
    11641 agacctgctt cggtggcttc ggcaatggca cggcgtagat tcactgccgc ttcgatatat
    11701 tcgccacgca ggaagatgta gccacggtaa gctttcagcg caaacgcgga gatgagcata
    11761 ccttccacca gcaggtgcgg cagttgctcc atcaacaggc ggtctttata ggtgcccggc
    11821 tccatttcat cggcgttaca cagcaggtaa cggatgttca tggattcgtc tttcggcatc
    11881 aggctccact tcaggccagt cgaaaagccc gcgccgccgc gccctttcag accagcgtct
    11941 tttacctgat taacgatttc gtccggagac agcccggtca gcgccttacg cgccccttcg
    12001 taaccatttt tgctgcggta ttcgtccagc cacactggct gtttgtcatc gcgcagacgc
    12061 caggtcagcg gatgcgtttc gggagtacgg ataatgtttt tcatttatac cgctccagca
    12121 gttcagggat cgcttccggg gtcagatgcg cgtgagtgtc ctcatcgatc atcatatttg
    12181 gccctttatc acagttcccc aggcagcagg ttggcagcag cgtaaagcga ccgtcaaacg
    12241 tcgtttgccc tggtttgatg ttcagttttt tctccagcgc cgcctgaata ccctggtaac
    12301 cgttgatgtg acagaccacg ctgtcgcaat aacggatcac atggcgacca accggttggc
    12361 ggaagatctg gctatagaac gttgccacgc cttcgacatc gctcgccgga atacccagca
    12421 catcggcgat cgcgtggatc gcgccgtccg gcacccagcc acgctgcttc tgaacgattt
    12481 tcagcgcttc aatggacgcc gcacgcgggt cttcgtagtg gtgcatctcg tgctcaatcg
    12541 cttcacgctc tgccgcactc agctcaaaag cctcggtttg tggttgttga ttctcgtgca
    12601 taattagcgg tccacatctg acataacaaa atcgatactg cccagataaa caatcaggtc
    12661 agacaccagg ctgccgcgga tcgccgccgg aatttgctgc aaatgcgcat aactcggcgt
    12721 gcggatacga gtgcgataac tcatggtgct accgtcgctg gtcaggtagt aactgttgat
    12781 ccccttggtc gcctcaatca tctggaaaga ttcattggca ggcatcaccg gcccccacga
    12841 cacttgcagg aagtgggtga tcagggtttc gatatgttgc agcgtgcgct ctttcggcgg
    12901 cggcgtggtc agcgggtgat ccgctttgaa cgggccttcc ggcatgttgt tgaggcactg
    12961 ctcaagaatg cgcagactct ggcgcagctc ttccactttc agcattacgc gggtgtagca
    13021 gtcagaaaca ccaccaccaa ccgggatttc aaagtcgaag ttttcatagc cagaataagg
    13081 acgcgcctta cgcacgtcga agtcgatccc ggtagcacgc aggcccgcgc cagtggtgcc
    13141 ccactccagc gcctctttcg cgccataggc ggcaacgccc tgggaacgac ctttcagaat
    13201 ggtattttgc agcgccgctt tctcgtaaga cgccagacgt ttcggcatcc agtcgaggaa
    13261 ctcacgcagc aggcgatccc agccgcgcgg caggtcgtgc gctacgccgc caatacggaa
    13321 ccacgccggg tgcatacgga aacccgtgat cgcttccacc agatcgtaaa ttttctgacg
    13381 atcggtaaag gcgaagaaca cgggcgtcat tgcgccgacg tcctgaataa aggtcgagat
    13441 gtacagcagg tggctgttga tacggaacag ttcggagagc ataacgcgaa tgacgttaac
    13501 gcgatccggc acggtgatcc cggccagttt ctctaccgcc agcacgtaag gcatttcgtt
    13561 aacgcagccg ccgaggtatt caatacggtc ggtatacgga atgtagctgt gccaggactg
    13621 gcgctcgccc attttctccg caccacggtg gtggtagccg atgtctggta cgcagtcgac
    13681 aatctcttcg ccgtcgagtt gtaaaacgat acggaaagcc ccgtgcgccg acgggtggtt
    13741 cggaccgagg ttgaggaaca tgaagtcctc gttttcggtg ccgcgcttca tcccccactc
    13801 ttccggtttg aaggtcaggg cttccatctc cagatcctgt ttggctttgg tcagctcaaa
    13861 cggcgagaat tcggtagcgc gcgccgggta atctttacgc agcgggtgac ctttccaggt
    13921 ttgcggcatc atgatgcggc gcaggttcgg gtgaccgtcg aaagtaatgc caaacagatc
    13981 ccaggtttca cgctcatacc agttagcgtt cgggaacagt ttggtgaagg tcggtacgtg
    14041 caggtcgttt tctgccagcg ccaccttcag catgatgtcg cggttacgat cgatagaaat
    14101 cagatggtag aaaacggaaa aatccgcggc aggtaaccct tcgcggtgtg tgcgcagacg
    14161 ttcgtccatg ccgtgtaagt caaacagcat gacgtaaggt ttcggcagtt tctttaagaa
    14221 atcgccaact tccagtaatt gttcacgctt gatccacaca acgggaaccc cggtgcgagt
    14281 cgcctgaaca gtaaaggcat ccggcccaaa acggttgcgc agttcgccaa ttaccggatc
    14341 atcaagatga tcgcgggtct gccaggcggg ttcttgcgcg gttaagtcgg tcatattgtt
    14401 caccattgca aatggtccgt ggtgactgtc ggcgcagtaa gcttcgcgaa atagatttag
    14461 gaataagcga agaaaaatcc ctttgccgac aggcgcaaat taaatctcgt caggtgtacg
    14521 caggttggtt acggcaatac gttcaccgcg cttgcgttcg cgctctgatt gcatattggc
    14581 gcgataaacg ccctgatcgc caaccaccca ggagagcgga cgacgttctt tgccgataga
    14641 ttcctgcaac agcatcagtg cctgcatgta cgcttcagga cgcggcgggc agcccgggat
    14701 atacacatca accgggatga atttatcgac gccctgcaca acggaataaa tatcgtacat
    14761 accaccagag ttggcacagg cacccattga gataacccat tttggttcca gcatctggtc
    14821 atacagacgc tgaataaccg gtgccatttt ggtaaagcag gttcctgcaa ccaccatcag
    14881 gtcagcctga cgcggcgaag cacgcaatac ttctgcgcca aaacgcgcca cgtcatgcac
    14941 cgcggtaaac gaagtcacca tctcaacgta acagcaggaa agaccgaagt tatacggcca
    15001 aattgagttt ttacgacccc agttaaccat gtcattgagc ttgcccataa acacgttttt
    15061 gttaacttct tgctccagag ggtcggttac gatctcctgc ttttgcaggg ggtaacggtc
    15121 gttctcaccg ttgggatcta tgcgggtgag cgtataatcc atcttaatgc ctcgcggtta
    15181 gcgttgacga ttagcgatac tgttcgtttc cgggttcata cgctcgcggc gtgaacgcgc
    15241 gggcgtccag tccagcgcgc caatacgcac cagataaacc agacctgcca gtaacacaaa
    15301 aataaaaatt gcagcttcca caaagcctac ccagccgctt tcgcggatag aggttgacca
    15361 tgcgaacaga tacagcgctt caacgtcgaa gataacgaag aacatggcca ccagataaaa
    15421 cttggcggac aggcgtaagc gggcggagcc gaccgagtcg ataccggatt caaacggcac
    15481 gtttttcgac ctcgcgcgtg cgcgaccgcc taaaaaccaa ccgcctacca gcatcaggca
    15541 acacaggcca atggcaacga taagaaagat agcgaatgcc cagtgatgag cgatgacttc
    15601 agtggatgtt gacatactca ttgcttactc atcaaaagta gcgccagatt cactgctctt
    15661 cacggcagat ggacgccaca tcgattcatg gggaggaata aaaaaaacct tacaatcact
    15721 gtagaaattc ttttatacag ctaattgatg tggtctttta ctcctttcta taaccttttg
    15781 tcaactttaa caaaagtttc ttcacattag tttacataat atcaacacca ttagcattta
    15841 atgccctttc accccagatc cttgacgact ccaggataat tagatgttgt tgaatcgtgt
    15901 ccgttgtgaa gcaatggaaa aaatacgggt ctattttgac aggaatttgt gtcgattcct
    15961 ccccccaaaa gagagtattt tcttgatctg tgacacgctt ttgtcattcc ataacaaaaa
    16021 cgcagcaaca aatttacgta ttttttaaca tcattgtagc aggtgatttt tttcaggcga
    16081 ttatttgtgc gttcgggacg tgaatctctg gtgattgaaa aatgaacagt tttgtacgtt
    16141 ctgcactatg cgaggaagga tttttactaa aaaaaagccg ctggggttta aaacaccccc
    16201 agcggctcgt tttttacact attgtctcag gaattatcta tcgtccgtcg attactcgat
    16261 atccctttca atcaacaatg aatcatcccc ttccggagca gacattgggc tgtattgcca
    16321 cggattatgg tagctctcca tcgcctgata gatcacctgg gccagctcat tattactgga
    16381 cgggtcatag cacagcaaat attcggtatc aggtagcggc ggtaagccat ctactccact
    16441 cagcacacgc aggtccgggc tcatcatctc aaccggcctt gccgtcacgc caagaccggc
    16501 tttcactgcc gcacgaacgg ccggaagcgt cgaggcgaca taagccagtc gccatggaat
    16561 atctgcttta ttaagcgtcg ccagcaccat atcgcgaaac ggacttggat catccagcaa
    16621 tacaagaggg atcggctcgc ctttttgcaa tacgtattcc gctgcgcagt accagtgtgt
    16681 tggcgaggtt cgcaggttca atgccttaaa cgctgaagga cgatgggtgg ttaccatcaa
    16741 atccacttcc tgggattcca gcatttctgc catataggca ttacgtttaa cgcggacatc
    16801 cagcgctaat ttcggataaa ccgaactcac gcgatttaac aggaaaggta agatcgtatc
    16861 ggcagattca tctgaagcac cgatagttaa cacgccctga agattactga acattaatga
    16921 tgagcaggcc tcatcattaa aacgcaggat tttcctggcg taaccaagaa gttgaatgcc
    16981 atgttcagtt aacagtttgt tgcgaccgtg acgagcgaac agttctttcc caacgagttg
    17041 ttccagacgc tgcatttgct gacttacggc ggactgagta cgacacacag cggcagctgc
    17101 ggcagcaaaa gtgttcagat cggcaacagc aacaaatgtt ctcagcagat cgaggtcgag
    17161 gttaattatc ggacgatttg cacttatcat atattatcac ttactggcgg ctcatactga
    17221 gctggttaat gctgtgcaca cacaaacaag caattccatt tgtaatgtgc ctccctggca
    17281 gtttcatccg gaacccggac gaaagtaaaa atgcatatga gttgcactaa aaaagcgact
    17341 cacattgttc cgttataatg cctgaagtag atcacagaat atatcttcag ggatcgcata
    17401 tctattaagt tactcactct tttctattta tgacatgcgc gtgtttgtat aaatgtaaat
    17461 gtgagtcctt gttccactct cgtgcagcat cgctggtcat acgcgaacac gtacaaacag
    17521 caatggtgag gcggcatcaa gagcagggat ccgtcattta tgcgagcatt ttaccccaaa
    17581 aaccttttat ttataagggt cattgcgaat tatctgatgc aaagttatgt tatgttaggc
    17641 aaagtaatct tctattatta ataagcacat caaaaccttt ttgaatatta aataataatt
    17701 aattagcatc atcctcattc attaattccg cttaacaata gtttcacacc ttgcccccat
    17761 tacgacacct cggaaatcat cacgctgtta aactctccgt aatgcggaaa tatcatcatc
    17821 gcgtttcatt agtgaattct taaatgaggc attttcacac aattatctta cagataaaaa
    17881 aaccagactt acaattaaga atcagaacaa ttcactatat aacattgcat gtaaagcata
    17941 tacacctcat tattttgtca ttattaagtt attaacagca caatcgagcc ttcccctctg
    18001 gcaaaatctt attctgcaga ccttcaaaac accgtcctgg gggagtacat tgttctaagc
    18061 tgacttccac ggcagggagt ggcgataaca gcaaaaaagg tcaagattca tgtcccccat
    18121 tgaaaaatcc agcaaattag agaatgtctg ttatgacatc cgtggtccgg tgctgaaaga
    18181 agcaaaacgc ctggaagaag aaggtaacaa ggtactgaaa ctgaacatcg gcaacccagc
    18241 cccgttcggt tttgacgcgc cagatgaaat cctcgttgac gtgatacgca acctgcctac
    18301 cgctcaaggg tattgcgatt ccaaaggtct ttactccgcg cgtaaagcca tcatgcagca
    18361 ctaccaggct cgtggcatgc gtgatgttac cgtggaagat atttacatcg gcaatggtgt
    18421 atcggagctt atcgttcagg caatgcaggc attgctgaac agcggggacg aaatgttggt
    18481 tcctgcacca gattacccac tatggaccgc ggcggtttcg ctttccagcg gtaaagcggt
    18541 gcattatctt tgcgatgaat cctctgactg gttcccggac ctcgatgata ttcgcgctaa
    18601 aattacgcct cgtacgcgtg ggatcgttat tatcaaccca aataacccaa ccggcgcggt
    18661 atattccaaa gagcttttaa tggagattgt ggagattgca cgtcagcata atctcattat
    18721 cttcgccgat gaaatttatg acaaaattct ttacgacgac gctgagcatc actcaattgc
    18781 gccgctggca cctgacctgc tgaccattac ctttaacgga ctgtcgaaaa cgtaccgcgt
    18841 tgcaggcttc cgtcaggggt ggatggtgtt gaacgggccg aaaaaacacg ccaaaggcta
    18901 catcgaaggt ctggaaatgc tggcttcaat gcgcctgtgt gctaacgttc ctgcgcaaca
    18961 cgccattcag accgcgctgg gtggttatca gagcatcagt gaatttatta cccctggcgg
    19021 tcgtctttat gagcagcgta accgcgcgtg ggaactgatc aacgatattc cgggcgtttc
    19081 ctgcgtgaaa cctcgtggtg cgctgtatat gttcccgaaa atcgacgcca aacgctttaa
    19141 cattcacgac gatcagaaaa tggtgctgga tttcctgttg caggaaaaag ttctgttggt
    19201 gcaagggacg gcattcaact ggccgtggcc ggatcacttc cgcattgtca cgctaccgcg
    19261 tgtcgatgat atcgagctgt ctttgagcaa gttcgcgcgt ttcctttctg gttatcatca
    19321 gctgtaatct taatttcact gccggagatt gcatccggca gcgttatccc gccacaatga
    19381 cctgatgatg tcatcatacg taaggtcact atgaaacaga gccatttttt tgcccatctc
    19441 tcccgcctga aactcattaa ccgctggccg ctcatgcgca acgtgcggac ggaaaatgtg
    19501 tccgaacaca gtttgcaggt agcgatggtc gcccatgcgc tggcagctat caaaaatcgg
    19561 aaatttggcg gtaatgtcaa cgccgaacgt atcgctttac tggcgatgta ccacgatgcc
    19621 tcagaagtgc tcaccggcga tctccctact ccggtgaaat acttcaattc gcaaatcgct
    19681 caggagtaca aggctattga aaaaatcgct cagcaaaaac tggtcgatat ggttccggaa
    19741 gagctgcggg atatctttgc gccgttaatt gacgagcatg catatagcga tgaagaaaaa
    19801 tcgctggtga aacaggcaga tgcactgtgt gcatatctga aatgtctgga agaactcgcg
    19861 gccggaaata atgaattctt gctggcaaaa acgcgactgg aagcgacgct tgaagcgcgt
    19921 cgcagccagg agatggacta cttcatggaa gtatttgttc ccagcttcca tctttcgctc
    19981 gatgagatta gccaggattc accgctgtaa gcagccggag tctgcgtcgc atcaggcaat
    20041 aagcgccgga tgcgacatca ggctcttgtc aaaacggaaa cagcatcggg atcatcacca
    20101 cacaaaccgc catcacgata atggtgaacg gtacccccaa cttcacaaag tcactgaagc
    20161 tgtaatttcc cggacctaaa accagtgtgt taacaggtga agaaaccggt gtcataaagg
    20221 cggcggatgc tgccatcgcc acgaccatcg cgaatggata aggcgacacc cccatcgttt
    20281 tggcagcagc cagcgcaatc ggagccatca acaccgccgt cgcggtatta gagataaata
    20341 gcccaataac cgccgacaag acaaacaaac accccagcat catatgtggc ccgtaaccac
    20401 cgccaatgtc catcagccct ttcaccgcca gcgcgacacc tcccgttttc tgtaatgcca
    20461 cagcaaacgg catcatccca acgatcaaaa taatgctcgg ccagtgaatg gatttatagg
    20521 cgctttcagc atctatacag cggaatttcc ccatcagcag gcaggcgatg atagcggcaa
    20581 caggattagg aatttcatct gtcagcatta acgccaccat tagcaccaga cagaaaatgg
    20641 catggggtgc ctggctgtgc gcgggcgatg cttcactcac ctcttccggt aagttcagcg
    20701 ctacgaagtc gcggccctgt ttggccagca taccgatcag tttccagtta cccacaacca
    20761 ggatgatatc gcccagcagc agaggctcat ccgccagcga accttccagc gccacgccat
    20821 tgcgctttag ccccaccaca ttcagtccgt agcgggtacg aaaaccaatt tcgcgcaccg
    20881 atttaccaat cagttctgac tcaggaatta atgaaatctc tgccatgccc acatcaaggg
    20941 cctggtcaga aaaatactcg ccgcgcagta ccatcggctc cagcaattgc tcactacaaa
    21001 attgccggag atcgacatca gccgcagaca tatcaataag caaaacgtca cgcgcgcgaa
    21061 attcagaaac cccattaacg ttcacgataa cgcgacgaaa acgccgccag cgttcaacac
    21121 cgatgacgtt agcgccataa cgctcacgta atttgagatc atccagccgt tgacctatca
    21181 ttggcgatcc ggggcgaata gccagacgtc gcgcacgccc ggtcagtcga tattcacgga
    21241 taagatcgcg aaaggttcga cgcgtccagc cttcgcgctg cggggtctgg gtatcccctt
    21301 tcagcatgaa acgcatcact aacatataca aaatacccag caccagcacg accaggccaa
    21361 taggtgttac gctaaagaaa ctgaagccgt gatagccttc acgcagcaat tcactgttga
    21421 ctaccaggtt cggcggcgtc gccaccagcg tcatcatgcc gctaatcagc ccggcaaaac
    21481 ttaacggcat catcagacgt gacggcgaag tttgcatacg catggcaacg cttaacacca
    21541 cgggaataaa gatagcgaca acgccggttg aactcataaa cgcgccaagc cccgcgacgg
    21601 tcagcatcaa caaaaccaac attttgattt cactgttgcc cgccactttg accagccatg
    21661 ttcccattac ggtggcaaca ccggtacgga ccaaaccatc gccaataata aacaaggcgg
    21721 caatcaggac aacgttagga tcagaaaagc cggaaaatac ttcggggacc gtcagcgttc
    21781 cgcttaatgc aaacgcgaca ataacaaaca aagcgaccgc atccatacgc actctgcccg
    21841 tcgcaaacaa gacgatggca acggccagta atgaaagaac ccaaatcaat tcaccgttca
    21901 caacttatcc ttgttaattg agggggatga cttgattctg ccataaaaaa gccccgacga
    21961 gacggggcta aatcatgatc aggtgtttca ctgaataata acatcgccat ttggctgttt
    22021 ggtcacagta atttgctcca gactgtggag gaccaaatcg acctcattca ggcgcggggt
    22081 atctgccgga gcgttaaccg caatgacatg gcaacccgcc gccaggccag aaagcacgcc
    22141 agcgggagca tcttccacca ccacacactc ctgcggcgca agccccagca gctgcgcacc
    22201 taacagatac gcatcaggtt ctggttttcc gcgcttcact cgctcagcgg ttacaaacac
    22261 ctctggtgcg ggaagcccag ctattttatg gcgcgctcgc gctaccggca tggagccaga
    22321 agtcacaatg gcccacggaa tacctgcttt attcaaatga ttgagtaagg cgattgcccc
    22381 cggaagcgcg gtaatacctt cggtttccgt ggcctcgatg tgctccagac gcgtaaactc
    22441 ggcggcaata tcagcctcgg atttgcccgc cataaaatgg cgcaaagagg tgatcgcctg
    22501 tttaccgtga atgaaagcca gcacctcttc cggcgctaac ccatgacgtc tggcccagtt
    22561 gctccacgcc cgttctaccg caggcaggga atccaccagc gttccatcaa gatcaaacag
    22621 aaaacctttg caccgcacgc gggcctcctc aggcgttgat aatttgatta atttcgttgg
    22681 cgctcaaatg gtactgacgc gggcaggcat gccacacatt aagcatgcgc tgatattttt
    22741 cccacattgg cgtctgggcg ttaaaaccgt gagttccggc gtcaaaatgg gtatagcgcc
    22801 cttccacatt aaccataaag cggacataac cgaggtaacg tgcttcagtg gcggcgtcaa
    22861 agccgaggaa ggtgacacga cgttcatcga tggattgctg gtcctgcaaa ttagaccagg
    22921 aaacatgcaa cgcatgatac atctccataa tgtcgatgat agtgcggcag gtttcttctt
    22981 tcagctcgcc aaactcgcga tccaattcac gcatctgtaa tccgtaacca cgctcaataa
    23041 ttgtttgcag gcgacggtaa cgttcagcat ttgccggatc gagcatagtc atcatcttgt
    23101 actgattaga caaaataaga cgttgcgcgt tggtcatttc cattgttgac tcctgtatca
    23161 ctatactacg gtgaaaaaaa agaaggctga gtatgccttc ttttatatgc gtaatcaggg
    23221 gtcaattaca aatcatcaag gaaagtttta tccagttgtt tgaaggcgcg cttaagcgtg
    23281 tcagctaatg cctggtaatc aggcttgcct tcaacgggtg ccaacacctg tccagactcc
    23341 tgcaatttac cgcgaacttc ataaaaccag ttaaggattg cagggggtaa tggcgttaca
    23401 gaacgcttgc ccagccacca caatccctgc atgggtaaac ttaaggcgaa cagcgcagtg
    23461 gcaactgccg gcccaagctg accgcccagg gcaatctgcc agcagagagt aaatacggcg
    23521 atcggcggca taaaacggat cgcataacgc gtcatcttga taacgcgatt ttcgacaaag
    23581 accggggcaa ggcgtttttc cagcggccac gtctttgagt aatgctgtcc ccggcgaaac
    23641 aagctaaaaa aattaacaga acgattatcc ggcgttgaca tgcttcacct caacttcaca
    23701 tataaagatt caaaaatttg tgcaaattca caactcagcg ggacaacgtt caaaacattt
    23761 tgtcttccat acccactatc aggtatcctt tagcagcctg aaggcctaag tagtacatat
    23821 tcattgagtc gtcaaattca tatacattat gccattggct gaaaattacg caaaatggca
    23881 tagactcaag atatttcttc catcatgcaa aaaaaatttg cagtgcatga tgttaatcat
    23941 aaatgtcggt gtcatcatgc gctacgctct atggctccct gacgtttttt tagccacgta
    24001 tcaattatag gtacttccat gtcgagtaag ttagtactgg ttctgaactg cggtagttct
    24061 tcactgaaat ttgccatcat cgatgcagta aatggtgaag agtacctttc tggtttagcc
    24121 gaatgtttcc acctgcccga agcacgtatc aaatggaaaa tggacggcaa taaacaggaa
    24181 gcggctttag gtgcaggcgc cgctcacagc gaagcgctca actttatcgt taatactatt
    24241 ctggcacaaa aaccagaact gtctgcgcag ctgactgcta tcggtcaccg tatcgtacac
    24301 ggcggcgaaa agtataccag ctccgtagtg atcgatgagt ctgttattca gggtatcaaa
    24361 gatgcagctt cttttgcacc gctgcacaac ccggctcacc tgatcggtat cgaagaagct
    24421 ctgaaatctt tcccacagct gaaagacaaa aacgttgctg tatttgacac cgcgttccac
    24481 cagactatgc cggaagagtc ttacctctac gccctgccgt acaacctgta caaagagcac
    24541 ggcatccgtc gttacggcgc gcacggcacc agccacttct atgtaaccca ggaagcggca
    24601 aaaatgctga acaaaccggt agaagaactg aacatcatca cctgccacct gggcaacggt
    24661 ggttccgttt ctgctatccg caacggtaaa tgcgttgaca cctctatggg cctgaccccg
    24721 ctggaaggtc tggtcatggg tacccgttct ggtgatatcg atccggcgat catcttccac
    24781 ctgcacgaca ccctgggcat gagcgttgac gcaatcaaca aactgctgac caaagagtct
    24841 ggcctgctgg gtctgaccga agtgaccagc gactgccgct atgttgaaga caactacgcg
    24901 acgaaagaag acgcgaagcg cgcaatggac gtttactgcc accgcctggc gaaatacatc
    24961 ggtgcctaca ctgcgctgat ggatggtcgt ctggacgctg ttgtattcac tggtggtatc
    25021 ggtgaaaatg ccgcgatggt tcgtgaactg tctctgggca aactgggcgt gctgggcttt
    25081 gaagttgatc atgaacgcaa cctggctgca cgtttcggca aatctggttt catcaacaaa
    25141 gaaggtaccc gtcctgcggt ggttatccca accaacgaag aactggttat cgcgcaagac
    25201 gcgagccgcc tgactgcctg atttcacacc gccagctcag ctggcggtgc tgttttgtaa
    25261 cccgccaaat cggcggtaac gaaagaggat aaaccgtgtc ccgtattatt atgctgatcc
    25321 ctaccggaac cagcgtcggt ctgaccagcg tcagccttgg cgtgatccgt gcaatggaac
    25381 gcaaaggcgt tcgtctgagc gttttcaaac ctatcgctca gccgcgtacc ggtggcgatg
    25441 cgcccgatca gactacgact atcgtgcgtg cgaactcttc caccacgacg gccgctgaac
    25501 cgctgaaaat gagctacgtt gaaggtctgc tttccagcaa tcagaaagat gtgctgatgg
    25561 aagagatcgt cgcaaactac cacgctaaca ccaaagacgc tgaagtcgtt ctggttgaag
    25621 gtctggtccc gacacgtaag caccagtttg cccagtctct gaactacgaa atcgctaaaa
    25681 cgctgaatgc ggaaatcgtc ttcgttatgt ctcagggcac tgacaccccg gaacagctga
    25741 aagagcgtat cgaactgacc cgcaacagct tcggcggtgc caaaaacacc aacatcaccg
    25801 gcgttatcgt caacaaactg aacgcaccgg ttgatgaaca gggtcgtact cgcccagatc
    25861 tgtccgagat tttcgacgac tcttccaaag ctaaagtaaa caatgttgat ccggcgaagc
    25921 tgcaagaatc cagcccgctg ccggttctcg gcgctgtgcc gtggagcttt gacctgatcg
    25981 cgactcgtgc gatcgatatg gctcgccacc tgaatgcgac catcatcaac gaaggcgaca
    26041 tcaatactcg ccgcgttaaa tccgtcactt tctgcgcacg cagcattccg cacatgctgg
    26101 aacacttccg tgccggttct ctgctggtga cttccgcaga ccgtcctgac gtgctggtgg
    26161 ccgcttgcct ggcagccatg aacggcgtag aaatcggtgc cctgctgctg actggcggct
    26221 acgaaatgga cgcgcgcatt tctaaactgt gcgaacgtgc tttcgctacc ggcctgccgg
    26281 tatttatggt gaacaccaac acctggcaga cctctctgag cctgcagagc ttcaacctgg
    26341 aagttccggt tgacgatcac gagcgtatcg agaaagttca ggaatacgtt gctaactaca
    26401 tcaacgctga ctggatcgaa tctctgactg ccacttctga gcgcagccgt cgtctgtctc
    26461 cgcctgcgtt ccgttatcag ctgactgaac ttgcgcgcaa agcgggcaaa cgtatcgtac
    26521 tgccggaagg tgacgaaccg cgtaccgtta aagcagccgc tatctgtgct gaacgtggta
    26581 tcgcaacttg cgtactgctg ggtaatccgg cagagatcaa ccgtgttgca gcgtctcagg
    26641 gtgtagaact gggtgcaggg attgaaatcg ttgatccaga agtggttcgc gaaagctatg
    26701 ttggtcgtct ggtcgaactg cgtaagaaca aaggcatgac cgaaaccgtt gcccgcgaac
    26761 agctggaaga caacgtggtg ctcggtacgc tgatgctgga acaggatgaa gttgatggtc
    26821 tggtttccgg tgctgttcac actaccgcaa acaccatccg tccgccgctg cagctgatca
    26881 aaactgcacc gggcagctcc ctggtatctt ccgtgttctt catgctgctg ccggaacagg
    26941 tttacgttta cggtgactgt gcgatcaacc cggatccgac cgctgaacag ctggcagaaa
    27001 tcgcgattca gtccgctgat tccgctgcgg ccttcggtat cgaaccgcgc gttgctatgc
    27061 tctcctactc caccggtact tctggtgcag gtagcgacgt agaaaaagtt cgcgaagcaa
    27121 ctcgtctggc gcaggaaaaa cgtcctgacc tgatgatcga cggtccgctg cagtacgacg
    27181 ctgcggtaat ggctgacgtt gcgaaatcca aagcgccgaa ctctccggtt gcaggtcgcg
    27241 ctaccgtgtt catcttcccg gatctgaaca ccggtaacac cacctacaaa gcggtacagc
    27301 gttctgccga cctgatctcc atcgggccga tgctgcaggg tatgcgcaag ccggttaacg
    27361 acctgtcccg tggcgcactg gttgacgata tcgtctacac catcgcgctg actgcgattc
    27421 agtctgcaca gcagcagtaa tctcgtcatc atccgcagct ttgcgctgcg gatatctgaa
    27481 ccggaaataa tcactatttc cggtttttta ttctcttaat ctgcattaat cctttctgat
    27541 tatcttgctt aactgcgctg catcaatgaa ttgcgccatt tcactttgca tacttaccac
    27601 tttgttttgt gcaagggaat atttgcgcta tgtccgcaac cactgaatcc aaaccaacaa
    27661 gaagatgggc aatgcccgat acgttggtga ttatcttttt tgttgctatt ttaaccagcc
    27721 ttgccacctg ggtagttccg gtggggatgt ttgacagtca ggaagtgcag tatcaggttg
    27781 atggtcaaac aaaaacacgc aaagtcgtag atccacactc atttcgcatt ctgactaacg
    27841 aagcaggcga acctgagtat caccgcgtac agctgttcac gacgggcgat gaacgcccgg
    27901 gcctgatgaa cttcccgttt gaaggattaa cctcaggatc gaaatacggg acagctgttg
    27961 gcatcatcat gtttatgctg gtgattggcg gcgcgtttgg cattgtgatg cgtacaggaa
    28021 ccattgataa cggtatcctg gcgcttattc gccatacccg cgggaatgaa attctcttta
    28081 ttcctgcgct gtttattctg ttttcacttg gcggcgcggt atttggtatg ggagaagagg
    28141 ccgtcgcctt tgccattatc atcgcaccgc taatggtccg gctgggctat gacagtatta
    28201 ccaccgtcct ggtgacctat attgccacgc aaatcggttt tgccagttcg tggatgaacc
    28261 cgttttgtgt ggtcgttgct caggggattg ccggcgttcc ggtgctttct ggctccgggt
    28321 tgcgcatcgt ggtgtgggtt atcgccactc tgattggcct gatctttacc atggtgtacg
    28381 cctcacgagt gaaaaagaat cctcttctgt cacgcgtgca tgagtccgac cgcttctttc
    28441 gtgaaaagca ggctgatgtt gaacaacgtc cgtttacctt tggtgactgg ctggtattga
    28501 ttgtcctgac cgccgtaatg gtctgggtga tttggggcgt gatcgttaat gcctggttta
    28561 ttccagaaat tgccagccag ttcttcacca tgggtctggt gattggcatc atcggcgttg
    28621 ttttccgcct taacggcatg acggttaata ccatggcttc atcctttacc gaaggggcgc
    28681 gaatgatgat cgcccctgcc ctgctggtgg gtttcgccaa agggattttg ctgctggtcg
    28741 gtaatggtga agcaggtgat gccagcgtgt taaataccat cctcaacagc attgccaatg
    28801 ccattagcgg tctggacaac gctgtcgcgg cctggtttat gttgctcttc caggcggtat
    28861 ttaatttctt tgtgacatcc ggttctggtc aggcggcgtt aaccatgccg ttactggcac
    28921 cgcttggcga tctggtcggt gttaaccgtc aggttaccgt gctggcattc cagtttggtg
    28981 atggcttcag ccacatcatt tacccaacct cagcttcgtt aatggcgacg ctcggtgttt
    29041 gccgggtgga cttccgtaac tggctgaagg tgggcgcgac actgcttgga ctgctgttta
    29101 ttatgtccag cgtcgtggtg atcggcgctc agttgatggg ctaccactaa aaatgttaag
    29161 agccgcaatt gcggctcttt tttcattctg ccgtttcagt ctctacggct tcatttttgg
    29221 cattgcgctt catccacaac gccagcgctt tcagcgagtc tggagtgaac tcatcgcagc
    29281 gtgcggtgat ttcttccggc gtcagccagc aaacttcact gacttcatct tcctgtaggg
    29341 cgaagggacc gtgagagacg cagctgaaca atgcgcccca gacacggcaa tttttatctt
    29401 cgaaatagaa ctgcccgtgc tcagcaaagg ggacaccggc aatgcccaac tcttcttccg
    29461 cttcgcgacg cgcggattcc agcagttgct catcggcctg gaccacaccg cctgcggtcg
    29521 catctaacat gccgggtaaa aagtcttttg tctcggtacg acgctggacc agaattttgc
    29581 ccatgccatc atgcacgacg atgtaagttg cacgatgacg cagacactgt gcccgcattt
    29641 gttcccggct ggcttgtgca atgacttcgt tctcttcatt gacaatatcc acccattcag
    29701 tacttgccaa acgacgctgt tccattatca ggaaaccttc tttttctggc gctcttacgg
    29761 cgcattttga gttgtgggta aattacggat taatcgcgac ctgcgcaatg atactttgat
    29821 cattgagtgc gattacacta agtacgtcat tatccagcat gccataactc gccggattac
    29881 cgcctttcgg aatactcacc gagccggggt tgaagtggaa aatctccccc cgctgttctg
    29941 ccactggcag atgggtatgg ccgtacacca gcacatcgtt ctggtttaaa gcaggcagat
    30001 tttccgggcc aaaaagatga ccatgcgtca aaaacagacg ttgtttttcc agtaatacct
    30061 gttgccacgg cgcggttatc gggaaatgca gcagcatttg atccacttcg ctgtcgcagt
    30121 tgccgcgcac agcgataacc ttatgtgcca cttcattaag ccgttcagcg actttggctg
    30181 gcgcgtaacc ctccggtaaa gcattacgcg ggccgtgatt caacacgtcg ccaaggatca
    30241 ccagccactg ggcaccgctt tgggcaaaca actccagaac acgttccgtc gccggtaacg
    30301 acccatgaat gtccgatgca aacatcagtt tcatcactca ctcctcgtcg aaaaaacgta
    30361 agctatgata ctggattctg tcgttgctat cagccagatt gcttcgccga aagtgcaata
    30421 aaacgctgga cagacgctcg ctgccactgg aatgtcgcat aatccaccag gcgttctggc
    30481 acctcatcgc catgtagtac caggcggtta atcattagcg ccagatcagt atcagcaatg
    30541 caccattcac caaataaatt cggctgaccc agtactaaca aatgttctgc catcgcaaac
    30601 agtttctctg cactggcttt tccctcggcc gttagtggcg cttttttcgc ccccgcaaag
    30661 acaacatccg tcggacgctc ttcgcggatg ggcatcagat cgctgcgcag ccaggcctga
    30721 atctgtcgtg cacgcgcacg attttctaaa tcaagcggat aaatacgttc ccaggtcggt
    30781 ggcgcaaatc gatcttccag atactccgca atggcagaag attcactcaa ctcaaaatca
    30841 tcgatttgta ataacggcac acggcgtgtc tgaccgtaac cttgccacgt cggctgcaaa
    30901 tgttcaccgc tgtcgaggtc gatggtcttg atatgaaacg acaggccttt ttcctgcaac
    30961 gccacccagg cggataacac ataaggggag aaaaagtggg catctgacca aagcgtgata
    31021 gcgggtttac tcattatgtc ctcggcaata aagggggctg gctatcaaac tatagagcct
    31081 ttttgtcact gtcacgtgac gaatactcac gtaagtcgcc gcgctttcta tacttgtggt
    31141 gttcagatga ttaaacaacc ggagctgcaa tgatcgatct ctatttcgcc ccgacaccca
    31201 atggtcacaa aattacgctg tttctcgaag aagcagagct tgattatcgc ttgattaagg
    31261 tagacctggg gaaaggcggt cagtttcgtc cggaattttt gcgcatttcg cccaacaaca
    31321 aaattccggc aattgttgat cattctcctg ccgatggcgg cgaaccgtta agcctctttg
    31381 agtctggtgc cattttgttg tatctggctg agaaaacagg actctttttg agtcatgaaa
    31441 cgcgtgagcg cgccgccaca ttacagtggt tattctggca ggtaggcgga ctggggccga
    31501 tgcttgggca aaatcatcat tttaatcacg cagcccccca aaccattcct tacgctattg
    31561 aacgttatca ggttgaaact cagcgtcttt accatgtact gaacaagcgg ctggaaaact
    31621 cgccctggct gggaggcgag aactacagca ttgcggatat tgcctgctgg ccgtgggtta
    31681 atgcctggac tcgccagcga attgacctcg caatgtatcc ggcagtcaag aactggcatg
    31741 agcggatccg ttcgcgccct gccaccgggc aggcactgct aaaagcacaa ctcggtgatg
    31801 agcgttcgga tagttaacag aaacaggttc tcgtgtatta tttcatccta agtaaaacaa
    31861 cggagaacct gcaatggcac aacctgccgc tattattcgt ataaagaacc ttcgtttgcg
    31921 tacgtttatc ggaattaagg aagaagaaat taacaaccgt caggatattg ttatcaatgt
    31981 gacgatccac taccccgccg ataaagcgcg cactagcgaa gatatcaacg atgcgctgaa
    32041 ttatcgcacc gtaacgaaaa acattattca gcatgtagag aataaccgtt tctctttgct
    32101 ggaaaaatta actcaggatg tgctcgatat cgcacgtgaa catcactggg tgacgtatgc
    32161 tgaagtggag atcgataaac tgcacgcgct gcgctacgcc gattcggtat ccatgacctt
    32221 aagctggcag cgttaaccgc catatcggga ggctgcatga acatagtgat caccggaggg
    32281 acgggattaa ttggtcgcca tttgattccg cgtttgctgg agctgggcca tcaaatcacg
    32341 gtagtgacgc gtaacccaca gaaagccagt tccgttctcg gccctcgggt gacgctatgg
    32401 caagggctag ccgatcaaag caacctcaac ggcgttgatg cggtaatcaa cctggccgga
    32461 gaaccgattg ctgataaacg ctggactcac gagcaaaaag agcgtctctg ccaaagccgc
    32521 tggaatatca cgcaaaaact ggtcgatttg attaatgcca gcgacacgcc gccgtcggta
    32581 ctcatttccg gctcggcaac gggctattat ggcgacttag gtgaagtggt ggttaccgaa
    32641 gaggaaccgc cgcataacga atttactcat aaactctgcg cccgctggga agagattgcc
    32701 tgccgggcgc aaagtgacaa aacgcgagtg tgcctgctgc gaaccggcgt ggtactggca
    32761 ccggacggcg gtattctcgg taaaatgctg ccaccgtttc gtcttggcct gggcgggccg
    32821 attggttccg gtcggcagta tctggcctgg attcatattg atgatatggt caacggcatt
    32881 ctctggctgc tggataacga cctgcgcggg ccatttaata tggtttcgcc ctacccggta
    32941 cgcaatgaac aatttgccca tgcgctcggt caagcgctgc atcgcccggc cattttgcgc
    33001 gtccccgcaa ccgccattcg gctgttaatg ggcgaatctt caatactggt attaggtgga
    33061 caacgcgcgc tgcctaaacg gctggaagaa gcgggttttg cgtttcgctg gtacgattta
    33121 gaagaggcgc tggcggatgt cgttcgctga tgtggtttac agcaaacatc cgccagttaa
    33181 ctcccggtgt tacaggatta gtggctttgc gcgataagat cgtctggtga aagtcgggtc
    33241 accatcataa ctaactctct gtctaaacct ctatccagca tctcctgagc aatacgcagg
    33301 gcttcttcgt gtttgccctg cattgcgcct tcttcacgta atctgtcagc aatggtcatc
    33361 agtttctcct tttcttgtgg tgcgcgttct gttatctcac caataaatgc acggaaacgc
    33421 tgggcatccc ctgtttgtaa tacgtaatta aacagggctt ttagctgtct gtcattagtg
    33481 ttccctgtaa ctagcagcga aacaatttgg tcgactaatc ccaacagatc gcgctgacga
    33541 atatgtttct gaattaactc caacagcgcc attttgcggt gttgcataat ctcgtcatcc
    33601 ggcaccacgg taatatccac caacggaaac gctgatgaat atattttgcg ggctatagca
    33661 ggctcggcaa attcatcaag ccagcagagt gaataaggat aaggacttct gcaaccatga
    33721 taaaacagca tcgggatcac caatggaagc tctttatagc ccgcatcaag atggttttgc
    33781 attgccgcaa tggaataacg catcatgcga aaagccatta attcttccgg cttactttgg
    33841 tgctctatca ctacataaat ataacccact ccctcctgcg ttttcacaga ccacaagagg
    33901 tcggaataat attgccgcag gtcgtcatca ataaaactgt ttggttccag tttaagcgtc
    33961 gttaaatcac acagtttgcg cagcggcgcg ggaagatgaa tatcaataaa atcccgcgcg
    34021 gtgtctggat ggcgtaaaaa agatttaaat accgcatcat gcggcgtgga agttgtcgat
    34081 attgtcatgg cgttccgtca cccctcaaat ttaagatgac gcgacaataa ccacgattaa
    34141 cacccaatgc agcaggcagt ttttatcttt ggacagcgaa ttcagaagta tttttagcgg
    34201 actgcaactt ttacatttca gcggaagctg actcacaaat tccccatgaa aatactccgg
    34261 cagcgttagc caccggagag gaaattattt cagcgatccc ttaaggaact gttgcagacg
    34321 agggctttgc gggttgccaa ataactgctc cggcgcgccc tcttcttcta tttttccctg
    34381 atggaggaaa atgacgtgag tagaaacatg gcgagcaaag cccatttcgt gagtcactac
    34441 caccatggtt ttcccctctt ctgccagttg ctgcataata cgtaacactt cgcctaccag
    34501 ttcaggatcg agcgccgagg taggttcatc aaacagtaaa acttccggtt ccatcgccag
    34561 cgcccgcgcg atagaaacac gctgttgctg accgccggaa agatgcaccg gatatttccc
    34621 ctgcgcacgt tcgtctatcc cgactttcgc cagatacttc accgcccgct cgcgcgcttc
    34681 ctgcttgctc aggcccaaca cctgaatcgg cgcttccatg acgttttcca gcaccgtcat
    34741 atggctccag agattgaagt gctggaatac catcgtcagg cgtgtgcgca gtaagcgcag
    34801 ttgattttta tcggcgactt tgagttgacc atctttgtcg cgcaccagat tgatcgtctg
    34861 gccattgacc acgatcgacc cttcactcgg tttttcgagg aagttaatgc agcgcagaaa
    34921 ggtacttttc cccgatcccg acgatccgat gatgcttatt acatctccgg cattcgcttg
    34981 cagtgagacc cctttcagca cttcatgttc gccgtagcgt ttgtgcaaat cgataacgtt
    35041 taatttattc tcggacatcg tattctcagt gcgttgaaga aggtttcaca tgctgcaacc
    35101 agcgtttttc cgctctgcga aagaggctga tcaggacata agagatgatt aaatagagca
    35161 ccgcggcaat gccgaaggcg gtaaaaggtt gatacgtggc ggcgttaata tcgcgggcta
    35221 ttttcagcag atccggcaca gtggccgtaa acgccagcgc agtagagtgc agcatcagga
    35281 tcacttcgtt gctgtatgcc ggtaacgcaa tacgcagcgc agaaggcaaa ataatgcaac
    35341 gatacatttt aaaagtcgag aagccatagg ctctggcggc ttcaatttcc ccatgcggaa
    35401 ccgaacggat tgccccagca aaaatctcgg tagtgtaagc gcaggtgtta agcgtcagcg
    35461 ccagcacggt acagttcagg ccactgcgga agaaagcgtt aaggaattcg gttcccttaa
    35521 caatctcaag cgtgtacatg ccggaataga acaccagcaa ctgaacatac agcggcgtac
    35581 cgcgaaaaat ataggtaaat aaccagattg gaaactggat gtatttatta ctggagacac
    35641 gaccaatcgc cagaaacagc gccaggactc cgcctatcac taccgacaaa ataagcagcc
    35701 acagagtgat cgccacacca gtaaagcgat aaccgtcggt ccacagcagc ggtttccagt
    35761 attcatgtaa gatttcgatc acaggtcagc cctcttcaca cccacggagt agcggcgctc
    35821 aaggaacagc agcacaccat tggaaacagt ggtgaaaacc aggtaaatca cgccacagac
    35881 gatggcgaaa tagaacggtt cccaggtact tttgcctgcc agttgggtgg ctttgaccac
    35941 atcttccagg ccgagtaacg aaaccagtgc ggtagatttg aggatcacct gccagttgtt
    36001 gccaatgcct ggcagggcgt aacgcatcat cgccggaaac atgatccgcc gaaacacttg
    36061 cccacgagta aaaccgaacg ccgtcgccgc ctctatatgc cctttcggca ctgccataaa
    36121 cgcgccacgg aacgtttcgg taaaataagc accgtagata aaaccgagag taataatacc
    36181 agcgaccatc ggatcgatat caatctgccc gacgcccatc gcctccgtca ccgtgtttag
    36241 tgcaatctgc aaaccgtaga aaatcagcag catcaacacc aagtccggca cgcctcgaat
    36301 cagcgtggtg tacccttcga aaatcagccc cgaaagccga ttttgcgaga gcttaccgcc
    36361 agcgccaatt aaaccgatga ttacagcgag cactacagaa ctgatagcca gctccagcgt
    36421 gacgagcgca ccctgtaaaa taacacctga aaacccatac aacatgctgc ctgtcctgtc
    36481 gtgtgtggtg aattaccgcc tcttgtctcc ctccggttac ccgaagggag cgggctttct
    36541 ttcaccgatg gcgattaacc gccataaaca tcaaaatcga agtacttttt cgctaatttc
    36601 tcgtaagtac cgtcagcgcg catttcggca aaggctttgt tcagtgcctc gcgcagttcg
    36661 ttatcttctt tacgcaggcc catgccggtc cctacgccaa acagtttttc atctttaaca
    36721 gacgggccac cgaatttgta atctttaccg acaggttgtt tgaggaaacc ttcgctggca
    36781 gcgacctcat cctggaacgc ggcatcaata cgtccggcag tcaggtcaga ataaatgttg
    36841 tcctgcccct gatacgagac gatttcaatg ccttttggtg cccaatgttc attaccgaac
    36901 gtctcctggg tggtgccctg caatacgcct acccgtttgc ctttcagcga ctcgactgtc
    36961 ggctgaatgt cggaattttt cgccaccacc aaacgagaat cggcagcgta cagtttgtcg
    37021 gtgaaggcta tttcttgctg acgtttttcc gtaatggaaa gcgatgacat gatggcgtca
    37081 atcttcttcg cttttaagga cgggattaac gcatccagcg gattttcgac aaacgtacat
    37141 tgcgtattga tgcgtttgca taattccttt gccagatcga tatcgaagcc aaccagttcg
    37201 ccttgtgaat tttttgattc aaatggcgca taggtcgggt cggtaccgat gcggatgttt
    37261 tgcggaatcg cagcaaacgc cgcagttgcg ctggagaagg ccagaaccag agagagcgat
    37321 agcaccagtt ttttcataat gtcctcaact gacagtcttt ttatagggaa tatttacagg
    37381 tttgttacac ttatcgtgcc ataaaattga ccatcaaggc aaaatatccc gccccgttag
    37441 acgtattatt tttcattaaa aatgcttaag tatgcatttc aatgcaccac gatagtgcaa
    37501 caaacctggc gtgcaccatg atgatcattc catcaggtac agcttcccag cgacgtatca
    37561 gtcaccgtag acattaaagt cgaaatactt tttcgccatc ttgtcgtagg tgccgtcctg
    37621 acgcagctcg ccaagcgcct tattgaaggc agccgtcagt tcagcatcat ctttacgtag
    37681 ccctacaccg gtgccatcac cgaagtattt tttgtctttt actgatgagc cagcaaaggc
    37741 gaaatcttta ccagcaggtt gcttgaggaa tccttcgctg gcagcaactt catcttgtaa
    37801 cgcagcatcc agacgtcctg cagccagatc ggaatagacc aaatcctggt tggcataggc
    37861 caccacatcc acgcctttgc tacgccaggt ctcgttagcg taagcttcct gggttgatcc
    37921 ctgcagcaca ccaacatgtt tacctttcag tgaatccagc gttggctgaa tcggtgaacc
    37981 tttggcagca atcaaacgag aatctgcggc gtacagcttg tcggagaagg caatctcctg
    38041 ctgacgttta tcggtaatgg aaagcgacga aataatggcg tcgatttttt tcgctttcag
    38101 tgaggggatc agcgcgtcaa agtcactggc aacccaggta catttcacct gcatccgttt
    38161 gcacatctcg ttaccgagat cgatatcaaa gccaacaaaa tcacctttag catctttcga
    38221 cgagaacggc gcataggtgg tatcggttcc gatacgtacc gtctccggta gcgccgcata
    38281 gctggaagcc gctgcggaga gaccgactaa caaagacaga gcgagaatcg acttcttcat
    38341 acataaccct caagtgaaaa tggctttttt atgttgtgta ttgtgttgtg tgtttgcagg
    38401 ctctttcatg caggtcttat gccatcttga cgacaacagt aacattcaac gttaaatatg
    38461 ttaataagac gttgcattat tgtcctgaag ttgaagatag caggtatagc ggttgaatcg
    38521 cagcgtttcg attgaatggc agaaacaaaa tgtcgaggat ttgatcgcgg ttgcaaaatt
    38581 gccctgaaac agggcaacag cggagttatg cgccctgcca gcgggcaaag agatcttcag
    38641 gaagggttat tgcaaactgg tcaagaacac gattaaccgt ctgatttatc acatcatcaa
    38701 gggattgcgg gcgatgataa aacgccggaa cgggaggcat aatcaccgca ccgatttctg
    38761 ccgcctgagt cattaaacgc agatggccta agtgcaatgg tgtttcacgc acgcagagca
    38821 ccaacgggcg acgctctttc agcaccacat ctgccgcacg ggtcagtaag ccatcagtat
    38881 agctatggac aatgccggaa agggttttga ttgaacaggg taaaatcacc atccccagcg
    38941 tctggaaaga accggaagag atgctggcgg aaatatcgcg cgcatcgtgc gtgacatcag
    39001 ctaatgcctg cacttcgcgc agggaaaaat ccgtttcgag ggataaggtc tggcgcgccg
    39061 cctggctcat caccagatgc gtttcgatat ctgtgacatc gcgcagaacc tgtaataagc
    39121 gcacgccata aatcgcgccg ctggcaccgc tgatgcctac aatgagtcgt ttcataaata
    39181 gttgccctgt cagacttgca ggcagacttt gcagtatttc ggcgggagtt gcaagtcagg
    39241 gtgccagacc ggcaccctca gcgaaggcat catccttcgt tatgcatttc gagattttcc
    39301 acttcgttct gacgttgcac tgctttggcg tcatcattac gtaacgtatc gaggaaatcg
    39361 aggtagccct gatcaacatc tttggtgacg tagacgccgt tgaacaccga gcattcaaac
    39421 tgctggatat ccggattttc agcgcgaacg gcgtcgatca gatcgttcag atcctggaaa
    39481 atcaacccgt cagcaccgat gatctggcga atttcatcaa cttcgcgacc gtgagcgatc
    39541 agttccgtgg cgctcggcat atcaatacca taaacgttcg ggaagcgaat ttccggtgcc
    39601 gcagaagcga ggtacacttt cttcgctccg gcttcgcgtg ccatctcgat aatctgctca
    39661 gaagtggtgc cacggacgat ggagtcgtcg accagcagga cgtttttatc gcggaactcg
    39721 gcgcggttgg cattcagttt acggcgcacg gacttacgac gcagctgctg gcccggcatg
    39781 ataaaggtgc ggccaacata gcggttttta acgaagccct ggcggtacgg tttgcccaga
    39841 atacgagcaa tttccagcgc gatatcacac gaggtttctg ggatcgggat caccacgtcg
    39901 atatccagat cttcccattc gcgggcaatt ttctcgccca gtttcgtgcc catattcaca
    39961 cgcgcgctgt aaacggaaat tttgtcgatg aacgagtccg ggcgagcaaa gtatacatac
    40021 tcaaacaggc acggattgct gaccggattg tcagcacatt gacgggtaaa caactgccct
    40081 tcttcagtga tgtaaatcgc ttcgcccggc gcgacgtcac gcaggaaatc aaagcccagc
    40141 gtatcgagcg ctacgctttc ggaagcgacc atatattctg tacggttctc gtcaatatca
    40201 cgttttccca gtaccagcgg acgaatcccg tttggatcgc ggaaagcaac cataccgtgg
    40261 ccgataatca tcgccacaca ggcatatgcg ccgcggatta agcggtttgt ggcagcaatg
    40321 gcagcgaaaa tattgtcggc ttccagcggg tagtggcgga agttgtccag ctcgctagcg
    40381 aagatattaa gcagaatttc cgagtcggaa gtggtgttga tgtggcggcg tttttcttca
    40441 aacagttttt tacgcaactc gtgagcgttg gtcagattgc cgttgtgggc aagcgtaatg
    40501 ccatacggag agttaacgta aaacggctgc gcttcagagg cgctggagct gccagccgta
    40561 gggtaacgca catgaccaat gcccatattg ccctgcaaac gctgcatatg gcgagcttca
    40621 aatacatcgc tcaccagccc gttcgcttta cgcaaacgga agcagttatt ggcatctatg
    40681 gtgatgatgc cggcggcatc ctgaccgcga tgctgaagca ccgttaaggc atcataaatc
    40741 gactggttaa ccggcataac accggcgata ccgacaatac cgcacatacg tctttttcct
    40801 cgttaagcca catctcagag cacttacgct ctgggcaaga aacttgacga gctttgcaga
    40861 taatcaaaaa accatctgat gataaaactg aattgcggga tcagctgtga tttgctccag
    40921 tcttcgcttt tcgacacccc ggtaaaggag tcgagaaaga agagaatggc ggcaacaatc
    40981 aacacaccgc gcaacgcacc gaaacagacg cccagcaccc gatcggtgcc tgacaacccc
    41041 gttttctcca ccaactggcc tatcacgaag ttcacgatag caccaacgat cagggtagcg
    41101 ataaacagta ccgcgatggc aattccattt cgaaccagtt cgtcttcaaa gcccgtaaac
    41161 cagactgaca ggtaagtgta gtaatgactg gcaacaaaga aagcacaacc ccatgtcacc
    41221 agcgataacg cttcacgaac aaagccgcgg atcaggctaa ccagagagga aaaagcaatc
    41281 accgcgatta tggcgtaatc aatccagacc atatgtgtcc cacgatttta cgccctgtca
    41341 tcctgttcgg ggcgcattct aacagaaaaa gaaaacgttt gcgtagggat ttccttcccg
    41401 cgcatcaata aaaatggcgc tgaaaaaata ttcaacgcca tcgacttttt atgcctttgc
    41461 ggcatcgtgc aatgcgtgtc ggatgcggcg taaacgcctt atccgaccta cggttctaac
    41521 actgcccagg cctgataaga cgcgccagcg tcgcatcagg caagactgta ttaattcggc
    41581 gtatagccca ttaccacgcc acttaagcca gaaagttgct tcaactcacc cagcgaacct
    41641 ttcagcttat ctttcgaggc atccggccca accagaatac gggtaatttt accctgcact
    41701 ggcgtggatg gcgacgtata aacccgataa ccggcaccgc gcagcttacc gacaatctca
    41761 ttcactttat cggcattttt cagcgcaccc agttgcacaa cataggcttt acccgttggc
    41821 gctgcttttt cttccacgac cggcttcggt tctggcttcg gcgcaggtgg tgcttcaacc
    41881 ttcggtttag gcggctccac cggtttcgac ttcggtgggg cgaccggtgc aggttccggt
    41941 tcaaactcgg tgttattggc tgcaatagtg gtcggatcga gcgacggtgc tgcggcatca
    42001 cctgcccgca cctcttccgc tgcgccttcc ggcggctgcg tcggcaacgc ctgggtggcg
    42061 gctggcatca tatcaggctc atcgcgatcg cccgctttcg gcaccagcgg gatagccgcg
    42121 aactcatcct gataatgttt tttctgcccg tccagcagcc ctggaagtac aatcaccccc
    42181 agcgccacca gcacgatcgt gcccactaac cgattctgaa acttacttgc caccgcttct
    42241 cctcgcgtca atcacttcca tgacatgtgc gacagtgtgg aaagaaccac acaccagcac
    42301 ggtgtcttcc gctttagcgt ccgccattgc ggcatcccat gcctgcgcaa cgctatcaaa
    42361 tgatttgccg ttacccaaat gctcaagcag ttgttctgcc gtggcaccgc gcggcccttc
    42421 cagtggcgca caataccagt catcaaccac gcttttcaac caggccagag ttccggcaat
    42481 atctttatca tgtagcatac cgataaccgc cagcacgcgc ccgtttttcg gtagcgcttt
    42541 catacgcccg gtgagatatt ccgccgcatg tggattatgc gcgacatcaa aaataacgcg
    42601 tggcgactcg ctcacaatct ggaaacgtcc cggcaaaatt gcgctggcaa tcccgtcgcg
    42661 aatggcattt tcactgactt ccagcccgct ggcacgcagt gccgccagcg ccgttgcggc
    42721 attcggttgc gggacaagcg gcaacggcag attttccagc gtgccgtgag catcgctaaa
    42781 cgcccagtca tgatcggtga cggaatagtt ccactcaacg ccccgacgtt gtaacagtgc
    42841 acctttttcc tgtgccacat cagcaatggt agaaggcatt tccggctcac cgacaattgc
    42901 cggtttttcg ctgcggaaga tgcctgcttt ctcgcgacca atactttcgc gatctggacc
    42961 cagccagtcg gtatgatcca gcgcaatact ggttactacc gcgacatcgg catcgacaat
    43021 attggttgcg tccagacgac cgcccagccc tacttccaga atcaccacgt caagttgtgc
    43081 ctgcttgaac agccacaacg ccgacagcgt accgtactcg aaataggtca gggaaatatc
    43141 accgcgtgcc gattcaatct ccgcaaaaga ggcggtgtgg gccgattccg gcaattcctg
    43201 gccctgcaca cgtacgcgct cggtataacg caccagatgc ggtgaactgt agacgcccac
    43261 tttgtaccct gccgccatca gaatcgactc cagcgtacgg caggtggtgc ctttgccatt
    43321 cgtacccgca acggtaaaca caaatggcgc tggtttcaga acgccaagac gcgccgcgac
    43381 ctggctcacg cgctcaaggc cgagatcgat agttttactg tgcaggtttt ccagataaga
    43441 aagccacgaa gccagaggcg acgcggcttg aggagtgcgt ttgataatca tggtatccgc
    43501 tgattcgtta cggtgagaat agcaaaaggg cagagccagt ggccctgccc ttatcagtta
    43561 tcaggcctca ggttcctgat ccggtaccgg gggtaccact acgccttcac gcggcgcttc
    43621 aggattcggc gctggcagat tcatcaactt cgccagaatg ctcgccagtt tcaggcgcat
    43681 ttccggacga cggacgatca tgtcgatcgc gcctttctcg atcaggaatt cactgcgctg
    43741 gaatcctagc ggcagttttt cgcgaacggt ctgttcgata acacgcggac cggcaaagcc
    43801 gattaacgct ttcggttcag cgatgttgag atcgcccagc atggcgaaac ttgcagaaac
    43861 accgcccatc gtcgggtcgg tcagcacgga gatgtacggc aagccgcgct cctgcatttt
    43921 tgccagtgcc gcagaggttt tcgccatctg catcagcgac atcagtgctt cctgcatacg
    43981 tgcgccacca gaggcggaga agcagatcag cgggcagtta tcttccagcg cctgctcaac
    44041 ggcacgcacg aaacgtgcac ccacaacaga ccccattgaa ccgcccataa aggcgaactc
    44101 gaatgccgca gcgacaaccg gcattccata cagagtgcct ttcatcacca ccagcgcatc
    44161 tttttcgcca gtttctttct gcgcagatgc cagacggtct ttatacttct tggagtcacg
    44221 aaacttcagc acatctttcg gctcaagctc gctacccagc tccacaaggc ttccttcatc
    44281 taacaggcta tgcaggcgat tacgcgctgt catacgcatg tgatggtcac acttcggaca
    44341 gacctcaaga ttacgttcca gctcagcgcg gtataaaacc tgaccgcagc tatcacactt
    44401 agtccacacc ccttcaggaa tgctcgcctt gcgggtggga gtaatgttgc ttttaattcg
    44461 ttcaatccag ctcattaggg acctttctgt ctgaacctgg ttcgatgcca gttttatctt
    44521 tggggacgca taatgccatt tttgccccca acagaccatg aatgttgcac attaaaacat
    44581 aacagcccga aactttggat aaaaaagtgg tcgaaccgcg gagttacttt ttattttgcg
    44641 gcgcgtgccg cagcgcgttt gtgacgaata aattcgaaaa ccccaggcaa tattgaaaca
    44701 aaaataatgc caacaatcat cagcttaagg ttgctctgaa tgaaggggag cgtgccgaag
    44761 aaataacccg cgtaggtaaa aatcagtacc cacaacagtg cgccgatcac gttataagcg
    44821 gcgaaatgac ggtacgacat gtggcccatt cccgcaacaa acggcgcgaa cgttctgacg
    44881 atcggtacaa aacgggcgag aataatcgtt ttgccgccat gtctctcgta aaactgatga
    44941 gtcttatcaa gataactgcg acgaaagatt ttcgaatccg ggttactgaa cagtttgtca
    45001 ccaaataacc gtccaatggt atagttcact gcatcaccga caatagcggc aatcaacata
    45061 agcaccacca tcatatggac attgagatcg ttagtttcca gcgacgccaa tgcaccagca
    45121 acaaacagca gcgaatcacc aggcaggaat ggcgttacca ccagccccgt ttcgcagaac
    45181 agaatcaaaa acagaatggc ataaacccag acgccgtact ccgcgaccag ctctgccaga
    45241 tgcacgtcaa tgtgcaggat aaaatcgatg aggaaataaa tcaggtccat atttgcctat
    45301 gccttgtact cgtcatcttt caggctgtaa ctacgttggc tacagcctga aatgttccga
    45361 gcattattct cgttagtccg ccagaaatag cgggcccatt ggcggttttg gaagatcata
    45421 ccggtcaggg taatccaccg cgaccagata cagcccttcc gcttttgccg ttgctgccgc
    45481 ccgcgttctg tcctttgccg ccagcaactc tgctatccag ctctccggct ggttgtgggc
    45541 accgacttcc atcaggctgc cgacaatatt cctgaccata tgatgtacaa aggcattcgc
    45601 tttgatatct accaccacat aaggaccgtg acgcatgaca ttaatatgca taacgttgcg
    45661 ccacggggtt cgggactggc actgcaccgc acggaacgag gtgaaatcat tctcgcccag
    45721 caagcattgc gcagcccgat gcatccgttc agcgtccagc ggttcgtaaa aatgggttac
    45781 ccctttactc agtaccgccg ggcgcagccg atgattgtag atgatgtagc gataacggcg
    45841 agccgtggcg ctaaatcggg catgaaaatc atcaggtaca gctttaaccc aacgcacggc
    45901 gatgtcacca ggtaaattcg catttacgcc caaggtccac gccgcgtctt tgcgctgcgc
    45961 ggttgtttcg aaatgcacca cttgcccggt accgtgtacc cctgcgtcag tacgcccggc
    46021 gcagaagacg gtgatgggtt cgttcgccac ctgggagagc gccttttcca gcttctcctg
    46081 cacactgcgg acttcattct gccgttgcca gccgtaatac ttactgccgt cgtactcaat
    46141 gcccagcgca attttataaa ctggcagttg ttgctggtcg gacattagta cagatactcc
    46201 tgcaccagtt tctcggcgat ttttactgcc atcagcgcgc cgccaaagcg aacgttatcg
    46261 gccaccgacc agaactggac ttgctccggc ataccgtagt cattacgcac gcagccaaca
    46321 gaaagatgcg gcgtacccga agcatcccct acctgagtcg ggaattcgtt ctcttcagag
    46381 agcacaatat cttcgccctg agcaaacgca tcacgcgctt cttctgccgc cagcggacgc
    46441 agtgcttcaa agttgaccat ctgggcatga ccgtagaata ccggtgcctg gacgacgctt
    46501 gccgaaatca tcagcccttc gtcctgcagg attttgcgta cttcgtcaac gatacgacgt
    46561 tcttcacgca cgctaccttc gctatccggc aacaacggca gcatattgaa cgccagttga
    46621 cgaccgaaga aatcttcttc gtcaatcgga atgccgttga gcaatttcgc actctgcccc
    46681 gctaacgcat cgaccgcttt tttgccctgg gcggaggctg aaatcaggct ggtaacactg
    46741 atacgcgaca aaccgccctg atcgattaac ggtttcagtg ccgccagcag ctggctggtc
    46801 agactgtctg gtacggcgat gacattccgg ttgcggtaat cggtcagtac aaacgggttt
    46861 acttccggca ccaccagcgg tacgtcgggt tcgagagcaa acagtccgct gctgtcgatc
    46921 accaggcaac ctgagttggt cgcttcttca acccaggcag cggtagcttc tttgcctgcg
    46981 acaaaaaatg ccagctgcgc ctgcgtccag tcgaattcag cggcatcctg cacggtgatt
    47041 gttttaccac caaagcgcag ttgttcgcct gcgctttcgt tacgtgccag tgcataaatt
    47101 tccccaaccg ggaactgacg ttcagccagc gtttcaagca gggcttcgcc cacagcgcca
    47161 gttgcgccca ggacggcaat gttccagcct tcagacatgg tggtttactc cagaaatagc
    47221 aaagctccct gccaatgtta cagcagagag catgaagaag agattaacgt gccggatgat
    47281 gaacggcgtt aaaacccagt ttacacagca atgatgccgc actggcgtca tcacaaatta
    47341 catacagaga tgaccattca cggcgctcaa gatagttttt gcgcagttta tcgaactcac
    47401 ccggtatccc ggcgacttta cgcagcggtg cgtcatcgcg gcgcacatca tacaccaaat
    47461 gcaccagcct tttcagcgtc ggttgatcga gcgggccatg cagcgtaatg cgaccaaact
    47521 ctggcgcggg cagtaatgta tccagcgcaa cgtgctgttc atgcccaata aacttgctat
    47581 aggcttcaaa cacttgcgta gtaccgcgtg ctttaccttc cagggtataa cctgcgatat
    47641 gcggcgtgcc gatatccact tttgtcagca actccacgtt aagttccggt tcgccttccc
    47701 agacatccag cactacgctt aacttctggc cttcattcag gcaggtcagc aacgcagtat
    47761 tatcgacgac tgcgccacgg caggcgttaa tcagaatcgc tcccggcttc aggctacgga
    47821 tcagtttttc atccgccaga tgcagcgttt tgtacggccc atctttgaag agtggcgtat
    47881 ggaaagtcag aatatccgca tgctgaacta attcatccag cgagcggaaa tcaccctcat
    47941 ccccacggtc agcgcgaggc ggatcgcaaa gcaatgtttt aatccctaac gcttccagtc
    48001 gcgcctgcaa tcgacgtcca acgttaccaa cgcccacgat ccccaccgta cggtcgtgca
    48061 gtgaaaatcc atcgcgttcg gcaagcatca gtagtgagga gaaaacatat tccaccaccg
    48121 caatcgcatt acagccaggt gcagcggaaa aaccaattcc cgcctgcttc aaccatgctt
    48181 catcgacatg gtcggtcccc gcagtggcag tgccaacaaa tttaatgggt tttcctgcca
    48241 gcaaagattc attcactttc gtgaccgaac gcaccatcag cgcatccgcg tctgccagtt
    48301 gagcaacggg gattggacgc ccgggaaccg cggtcacctc acccaaacgg ctaaataagt
    48361 cgcgggcata aggcatattt tcatcaacaa ggattttcac gtttgtgtta cctgtatgag
    48421 acgagagtta accggacaag tgtgccataa tctcgcggcc aggcatactt gcgaagattt
    48481 caggtataag gatacgtaat gatacaacct atttccggcc ctcctcctgg gcaaccacca
    48541 ggtcagggag ataacctgcc gtctggcgcg ggcaatcagc ctttatccag tcagcaacgt
    48601 acttcgctgg aaagcttaat gacgaaagtg acctcactga cgcaacaaca aagagcagaa
    48661 ctgtgggcgg gtatcaggca cgatattggt ctgtcgggag attcaccgct gctttcgcgt
    48721 cacttccctg ccgctgagca taatctggcg caacgtctgc tggccgcgca aaaaagccat
    48781 tctgcccgcc agcttttagc gcaattaggg gagtatttac gtctggggaa taatcgtcag
    48841 gcggtcacgg attatatccg tcataacttt ggtcagacgc cgctgaatca gctctcaccg
    48901 gagcaattaa aaaccattct caccctgttg caggaaggga agatggttat tccgcaacca
    48961 cagcagcgcg aggcgaccga ccgtccttta ttaccggcgg agcacaatgc gctaaaacag
    49021 ctggtgacca aacttgcggc ggcaacgggg gaacccagca aacagatctg gcaatcgatg
    49081 ctggaacttt ccggggtgaa agatggcgag ttaattccag cgaaactgtt taaccatctg
    49141 gtgacctggt tgcaggcgcg ccagacgtta agccagcaaa atacgccgac gctggaatca
    49201 ctacagatgg cgctaaaaca acctttagat gccagtgaac tggcggcgtt atcggcatat
    49261 atccagcaaa aatatggcct ttctgcgcaa tcatcgcttt cttctgccca ggccgaggat
    49321 attcttaatc agctttatca acggcgggtt aaagggattg atccgcgtga tatgcaaccg
    49381 ctgcttaatc cttttccacc gatgatggac acgttgcaaa atatggcaac gcgtcccgcg
    49441 ctgtggatac tgttagtcgc gattatcctg atgctggtct ggctggttcg ttagccccga
    49501 cgaaatgaca gtatcgtgac aataattccc agcaccgcag agatcgcccc ggcaagaaat
    49561 accgaagagt aaccaaacgt ggtcgccagc attcccgcca gcggcccgga gacgccgagg
    49621 gcgatatcct gaaacgcggc gtaaccgccc agtgcggtgc cgcgaacttg tgaggggacg
    49681 cgtttaacca cctccacgcc cagcgcagga aagataagcg aacatccggc tccggttaac
    49741 gccgcgcccg ctaatgcgac ccacgcacct ggggcttgcc agagcagcaa caagcccacc
    49801 gtttctacaa gcagagagac aatcgccact ttcacgccgc caaaacggtc cggcatccag
    49861 ccaaacatga cgcgcatcac gacaaatgcg ccgccaaacg cggtaagagt aaagcccgcc
    49921 atcgcccatc ctttgctggc aaagtagagc gaaacgaaag tcccgataac cgcaaaacca
    49981 acgccttgta gtgccagccc taaccctggt ttccagataa acccgacaac gctccacagc
    50041 gatggacgtt ctcccgccag ggccggtact ttgcgcactg tgccgttaca ggcccacgcc
    50101 agtaagggta ataccattgt ggtgatcgcc agtgcggcaa aaccgtaatg gctatgaatc
    50161 aacaggccaa gcggagcacc aacagcgagg gcaccgtaaa tcgccattcc attccatgac
    50221 atcactttgc cagagtgttt tggccctacg atgcctaacc cccaggtcag agcgcctgtc
    50281 agtaactggc tttcaccaaa cccaagaatc aaacgcccga tgaccaacag ggcaaatttg
    50341 aacggtgcgg agacaggcaa aatcgccgcc agcagcaacg cgccgccagc cagaccacaa
    50401 gctaacatcc cctgaagcgc cgaacgtttt gcaccatatt gatcggccag tcgcccggca
    50461 tagccacgcg tcagcaccgt agccagaaac tgaatcccga cggcaatccc gaccatggta
    50521 ttgccatagc ccagatcatg atgaacaaac agcgggataa ccggcaacgg cagccctacg
    50581 gtcatgtagg tgagaaaaac cgcaaaagcg atgcggaaga gcgaaaaatt ggcagaagat
    50641 cgtgtttcgg tttggcttac agcagtcatg cattactcca gaatgcagcg caaggcgagg
    50701 agtatccccg tctcatctct ctggtttcag ggttacggtg cgttggcagg atttaacgcg
    50761 tacgtctttt cagaaggaaa tcgacaaagc gggaagtttg cctggaacag gcggcgattg
    50821 tcaatgatgt gaaaaaggga accatcaggt tcccttttgc gttagtgccg gaggccgcat
    50881 tagcgcgtaa cgtcggatgc gacgctggcg cgtcttatcc gacctactcc gactactccg
    50941 gcctactgcg aattaatctt tcagcttgcg cattaccagc gtggcgttgg tgccgccgaa
    51001 gccgaagctg ttagacataa cggtggtcag ttcgcgatcg gtcgtttcgg tcacgatgtt
    51061 cagacctgca gcctgctcgt ccagctcttc aatgttgatg ctcggggcga taaagccgtg
    51121 ttccagcatc agcagagagt agatagcttc ctgtacgcca gcagcgccca gagagtgacc
    51181 ggtcatggct ttggttgcag aaatcgccgg gctcttatcg ccgaacactt cacggatagc
    51241 tgccagttct ttcacgtcgc caaccggagt cgaagtacca tgagagttca ggtaatcgat
    51301 tggggtatca acgccgtgca tcgccatctt catgcagcgt actgcgcctt cgccagacgg
    51361 agcaaccatg tctgcaccat cagaggttgc gccgtagcca acgatttcag catagatgtg
    51421 agcaccacgc gccagcgcgt gttccagctc ttcaaccact accataccgc cgccgccagc
    51481 gataacgaaa ccgtcacggt gagcgtcgta agtacgggag gctttttccg gggtgtcgtt
    51541 gtatttagta gacagcgcac ccatcgcgtc gaattcgcag gccatttccc agcacagctc
    51601 ttcgccgccg ccagcaaaca cgatgtcctg tttgcccagt tggatctgct ctactgcgtt
    51661 accgatacag tgtgcggaag tcgcacacgc ggagctgatg gagtagttaa cgccatgaat
    51721 tttaaacggc gtggcgaggc aggcagaaac gccggatgcc atcgctttgg tgaccacata
    51781 cgggccaacc gctttcaggc cgcgcgggcc gcgcattgcg tcagcgccga acacctggaa
    51841 acgcggggaa ccgccgccag aacctgcaat caggccaacg cgcgggttat tctggtaagc
    51901 ttccggagaa aggcccgcat ctgcgattgc ctgctccata gaaaggaatg cataaatgga
    51961 tgcgtcgctc ataaagcgca caactttgcg gtcaatgagg ccagtggtat ccagttttac
    52021 gttgccccag acgtggctac gcatgccgga atccttcagc tcctgagaga aagtgatccc
    52081 tgaacgtcct tcacgcagag atgccaggac ttcctgctgg ttattaccga tgctggaaac
    52141 aatgcccagg ccagtaatca ctgcacgttt cattcaatac ctctgtaagt cgcacataga
    52201 gtaagtttcg aatgcacaat agcgtacact tgtacgccga acaagtccga tcagccattt
    52261 aatagagaaa tttgcgcagc cttacacaca tcgctaagat cgagccaccg cctgtaagac
    52321 gagtaactta cgtgaaacac tactccatac aacctgccaa cctcgaattt aatgctgagg
    52381 gtacacctgt ttcccgagat tttgacgatg tctatttttc caacgataac gggctggaag
    52441 agacgcgtta tgtttttctg ggaggcaacc aattagaggt acgctttcct gagcatccac
    52501 atcctctgtt tgtggtagca gagagcggct tcggcaccgg attaaacttc ctgacgctat
    52561 ggcaggcatt taatcagttt cgcgaagcgc atccgcaagc gcaattacaa cgcttacatt
    52621 tcattagttt tgagaaattt cccctcaccc gtgcggattt agccttagcg catcaacact
    52681 ggccggaact ggctccgtgg gcagaacaac ttcaggcgca gtggccaatg cccttgcccg
    52741 gttgccatcg tttattgctc gatgaaggcc gcgtgacgct ggatttatgg tttggcgata
    52801 ttaacgaact gaccagccaa ctggacgatt cgctaaatca aaaagtagat gcctggtttc
    52861 tggacggctt tgcgccagcg aaaaacccgg atatgtggac gcaaaatctg tttaacgcca
    52921 tggcaaggtt ggcgcgtccg ggcggcacgc tggcgacatt tacgtctgcc ggttttgtcc
    52981 gccgcggttt gcaggacgcc ggattcacga tgcaaaaacg taagggcttt gggcgcaaac
    53041 gggaaatgct ttgcggggtg atggaacaga cattaccgct cccctgctcc gcgccgtggt
    53101 ttaaccgcac gggcagcagc aaacgggaag cggcgattat cggcggtggt attgccagcg
    53161 cgttgttgtc gctggcgcta ttacggcgcg gctggcaggt aacgctttat tgcgcggatg
    53221 aggcccccgc actgggtgct tccggcaatc gccagggggc gctgtatccg ttattaagca
    53281 aacacgatga ggcgctaaac cgctttttct ctaatgcgtt tacttttgct cgtcggtttt
    53341 acgaccaatt acccgttaaa tttgatcatg actggtgcgg cgtcacgcag ttaggctggg
    53401 atgagaaaag ccagcataaa atcgcacaga tgttgtcaat ggatttaccc gcagaactgg
    53461 ctgtagccgt tgaggcaaat gcggttgaac aaattacggg cgttgcgaca aattgcagcg
    53521 gcattactta tccgcaaggt ggttggctgt gcccagcaga actgacccgt aatgtgctgg
    53581 aactggcgca acagcagggt ttgcagattt attatcaata tcagttacag aatttatccc
    53641 gtaaggatga ctgttggttg ttgaattttg caggagatca gcaagcaaca cacagcgtag
    53701 tggtactggc gaacgggcat caaatcagcc gattcagcca aacgtcgact ctcccggtgt
    53761 attcggttgc cgggcaggtc agccatattc cgacaacgcc ggaactggca gagctgaagc
    53821 aggtgctgtg ctatgacggt tatctcacgc cacaaaatcc ggcgaatcaa catcattgta
    53881 ttggtgccag ttatcatcgc ggcagcgaag atacggcgta cagtgaggac gatcagcagc
    53941 agaatcgcca gcggttgatt gattgtttcc cgcaggcaca gtgggcaaaa gaggttgatg
    54001 tcagtgataa agaggcgcgc tgcggtgtgc gttgtgccac ccgcgatcat ctgccaatgg
    54061 taggcaatgt tcccgattat gaggcaacac tcgtggaata tgcgtcgttg gcggagcaga
    54121 aagatgaggc ggtaagcgcg ccggtttttg acgatctctt tatgtttgcg gctttaggtt
    54181 ctcgcggttt gtgttctgcc ccgctgtgtg ccgagattct ggcggcgcag atgagcgacg
    54241 aaccgattcc gatggatgcc agtacgctgg cggcgttaaa cccgaatcgg ttatgggtgc
    54301 ggaaattgtt gaagggtaaa gcggttaagg cggggtaatc tgctctcgca gtgtttgccg
    54361 gatgcggcgt gaacgcctta tccgacctac gtgtgacgtg taggcctgat aagacgcgat
    54421 aagcgtcgca tcaggcatgg tgctccagac gccgcaactt actgctgtga cgcctgctga
    54481 aacaggtttt cccacatatc ggtcaccagc gcctggtcac gcggcgacaa ttcaccggca
    54541 ccaatggctt tttccagact ctggctaacg gtcgtatgca ccgcctgagc ggagtggtca
    54601 tcaccacttt ccagttctgc gatggctaat gtcaggtggc cacgcaaata cccactggca
    54661 aacaactcat catcactggc atggtcaacc ataccgtcga ttaatgccag aatgcgtgat
    54721 tcaaactccg cgatcatctt ctttcctcat tgtaaaacgt ggcaccggct tcagttgagc
    54781 gcttccggcc acgggaactg ttccgccgta agttccggcg tgtgataata attttgtagt
    54841 gctttaatga agcgtgccgg gcgttcagga atgccgtttg ctaggtaatc catcacctgc
    54901 gcatgtaccc gccgttgaaa caccacacga tccggttcga agtcgccttc cagattgtcg
    54961 caactgacgt taaacggata tcccgccgcc acacagaaca accagtcgaa cgcctgcggc
    55021 ttcacttcaa catcttcaaa ctggctttgc gtttgggcat cgcgtccgtc cgggcaatac
    55081 cagtagccga aatcaaccag ctcacggcgc gctttcccag caatacacca gtgcgaaatc
    55141 tcatgaattg cgctggcata aaagccatga gcaaagacga ttcggttata cggtacttcc
    55201 gcatcagcag gaagatagat cggttcgtcg tcgcctttaa tcagacgggt attaaattca
    55261 tcggcaaagc agctattaaa aatttcaatc aactgctcat agtggtgtgt actgttcatt
    55321 agttcatccc caaccagtgg aggatctcct gtccgtggct atcaaaaagt aatttggcac
    55381 tcatcaccgc tgagacaata acaatcatcg ggcggatcag cttttgtcct ttgctcaaca
    55441 ccagtcgtga acccatgcgc gcgccgagga actgccccac cagcatgaca aagcccgtcg
    55501 cccaaatcac tttgccaccg agaataaaca gcagtaaacc gccgatgttt gacgttgcgt
    55561 tgagtaattt ggcgtgcgcc gtggctttgg cgaggttgaa tccgcacagc gtaacaaagg
    55621 ccagtgcgta aaacgatccg gctgccggac caaagaatcc gtcataaaaa ccgacgcagc
    55681 caccggcaat taacgcgaag ggtaaaccgt acatccgacg ctggcgatct tcttcaccca
    55741 gctttggcat cagcaagaaa tagaggccga tacaaattac cagaatgggc aaaatctgcc
    55801 gtaagacatc agcctgaacg tattgcacca gcagtgcgcc gctcattgag ccgacaaagg
    55861 tcatggcgat attgagtttc tgatcgctta aactaaccac tttgcggcga ataaagtaga
    55921 tagtagcgga aatagagccg ccgcaggctt gcagtttatt ggttgccagc gcattagcgg
    55981 gagacatccc cgctgccatc aatgccggaa tggtgagtaa cccaccgcca ccggcaatcg
    56041 agtcgataaa tcccgccagc atggcgacaa aaaagagaac tcccagcaac agcggggaaa
    56101 ccataaacag gctattaaac gtttccatta gatcacgtgc tcatccagta gcgcctggca
    56161 ggaaggcggc aacggaggcg gtgtcttctt ctcaggcttt gttgttcccg gttttggagg
    56221 ttcaaaccag ctttgcagtt ctgccccgca accatcgcct ggtggcggta aaggttgatc
    56281 ttcacactcc agactatcgg caggacaacg taatcgtaca tgcatatgcg cacgatgctg
    56341 gaaccagggt cgcactttgc gcaaccagtc gcgatcggtg cccgcatcta ggcaaagttg
    56401 ttgtttaatc gccggattaa caaaaatgcg cgtgacgttt ttgtcctggg cggcgagttt
    56461 gatcaagctg aaaatttctg gcttccacag cgaggagaca acgtgtttac cgtcgcggga
    56521 aactaagtcc agtgcttgcg ggcgcaagag ctgcgcggag gtccagcgag ttttcggcag
    56581 ttgcagaaag atatcgacat ccagtccggt ctggtggctg gcatgaccgc cgttgaaacg
    56641 cccaccagca ggcatcccca tatcgccaat cagcaccgta cccatgccca gattgctcac
    56701 ctggctactc agacgctgga taaacatcac cagatccggg tgaccgaaat agcgacgctg
    56761 atcggtacgc atgacctgat aatgttcgga ctgtatcggc agcgtgtcag cgccgacaat
    56821 acagccatta gaaaaactgc ctatcgattg tgcgctaccc ggcacaggtt gggttatttt
    56881 ttgccacggc gtcgctgcca ggctagcgct actggcaagc agagccagca gcgcaatcgc
    56941 ggttttgttc attttttacc agcgtggaat atcagtcttc acatcggcat tttgcgcccg
    57001 ctgccgtaac aggtgatcca ttaaaacgat cgccagcatc gcttctgcga tcggcactgc
    57061 acggatcccg acacagggat cgtgacggcc tttggtgatc atctcaactt cttcgccaaa
    57121 gcggttaata gtgcgccccg gcacggtaat gctggaggtc ggtttcagcg ccatatgggc
    57181 aatgatttgc tgcccgctgc tgataccgcc gagaatgccg cccgcatggt tgctctggaa
    57241 accgtctttg gtgatttcgt cgcggttctg gctgccgcgc agcgccacta cgtcaaaacc
    57301 gtcgccaatt tccacgcctt tcaccgcgtt gatgctcatc agcgcatggg cgatgtcggc
    57361 atccaggcgg tcaaagaccg gctcgccaag tccggcgggg acaccactgg caacaacggt
    57421 gactttcgcg ccgatggagt cgccctcttt tttcagcgcg cgcatcagtt catctaacgc
    57481 gtcgattttg tccgggtccg ggcagaagaa cggattttgt tcaaccagcg accagtcttt
    57541 gatttccagc ggaatgtcgc ccatctgggt caagcagccg cggatttcaa taccaaattt
    57601 ctcggcgaga tattttttgg caatcgcccc tgccgccacg cgcatggcgg tttcgcgggc
    57661 ggaagaacgt ccaccgccgc gataatcgcg cagaccgtat ttttgttcgt aggtgtaatc
    57721 agcatggcct gggcggaaaa cgtctttaat tgcgctgtaa tcctgagagc gctggtcagt
    57781 gttttcgatc aacaagccaa tgctggtgcc agtagtaacg ccttcaaaaa caccggagag
    57841 aattttgacc tgatccggct cgcggcgctg ggtggtatag cgcgatgtcc cagggcgacg
    57901 acggtcgagg tcatgttgca ggtccgcttc cgtcagcgga atgcctggcg gaacaccatc
    57961 gacgatgcag ccgagcgcca gcccatgcga ttcgccgaag gtggttacgc gaaagagttg
    58021 tccaattgtg tttccagcca tcacggctcc gttattgttg tgtttgcgtg tttacttaat
    58081 ctttataaat cgcgaaatat tcacgggccg ccagcagctg ctctttggtg agcataaaca
    58141 caccatcgcc gccgttatca aactccagcc aggtgaacgg aacatccgga tattgttcca
    58201 taagatgtac catgctgttg ccgacttcac aaatcagcac accatcatca gcaaggtaat
    58261 ctgccgcgtt accgagaatg cgacgcgtca gtttcaggcc gtcggtgcca gatgccaggc
    58321 ccagttccgg ctcgtgacgg tattcgtttg gcaggtcgga catatcttcc gcgtcgacat
    58381 acggcgggtt agtgacaatc agatcgtatt gcactttcgg caaatcacgg aacagatcgg
    58441 aacgaatcgg aatgacgttg tggatcagac cgtgttcttc gatgttctgt tcggcaaccg
    58501 ccagcgcgtc gggagagata tctaccgcgt cgacttccgc ttccgggaag gcataagcac
    58561 aggcaatggc gatgcagccg ctaccggtac acatatctaa aatatgctgc ggttgcttgc
    58621 tgatgagtcc ggcaaattta ttgttgatca gttcaccaat cggtgagcgc ggcaccagca
    58681 cgcgttcatc gacgtaaaat tcatgaccgc agaaccaggc tttgttggtc aggtaagcca
    58741 ccggaatgcg ctcgttgacg cggcggatca cgcgttcaac aatacggtgt ttttcgctgg
    58801 aggtcagacg cgcggtgcgc atatcttccg ggatatccag cggcaggtag agcgatggta
    58861 acaccagctg tacggcttca tcccacgggt tatcggtgcc gtgaccgtac cagatatttg
    58921 ccgcgctgaa gcggcttacc gaccagcgca acatgtcctg aatggtttgc agctcattta
    58981 ctgcttcatc aacgaaaatt ttatccacgt attcctccag ggcatgatcg caataatttc
    59041 ggcggctagt ttgccatgaa gatgacgata aatcagcatt cacgcgcggt gagtgaggaa
    59101 aaatacgttt aaaacgatcg attgtgctac gagtcgggta aactgtagga aaattagaaa
    59161 tagagacaga taaatgaaaa agaaaacaac acttagcgag gaggaccagg ctctgtttcg
    59221 ccagttgatg gcggggactc gcaagattaa gcaggacacg attgtccacc gaccgcagcg
    59281 taaaaaaatc agcgaagtgc cggtgaaacg cttgatccag gagcaggctg atgccagcca
    59341 ttatttctcc gatgagtttc agccgttatt aaataccgaa ggtccggtga aatatgttcg
    59401 cccggatgtc agccattttg aggcgaagaa actgcgccgc ggcgattatt caccggaatt
    59461 gtttttggat ttacacggtc tgacgcagct gcaggccaag caggaactgg gcgcgttgat
    59521 tgccgcctgt cgccgcgaac atgtgttttg tgcctgcgtg atgcatgggc acgggaagca
    59581 tattttgaag caacaaacac cgctgtggct ggcacaacat ccgcatgtga tggcatttca
    59641 ccaggcaccg aaagagtacg gcggtgatgc ggctttgttg gtgttgattg aagtggaaga
    59701 gtggttgccg ccggagttgc cctgaggagt tgagcgtgtt gtgccaggag cgcggaatgg
    59761 caggggcgaa tgaccctgcc aggtgagaaa aggtgcgcta atacatattc agtatgtaag
    59821 tgaatcggca ggtagaatct atgggctgtc gagatgtcgt gtccacgcgt agcttcatat
    59881 catgcagttg cctggacgtg atcggttgcg gctctggcgt caccgtcact ttggcattat
    59941 cacagccgga agtcgagaag gtcatctgct gtgcgacaaa acggcagtag gctccacgct
    60001 cgtcaaaatt actgccatca cacagtttgc cgttttccat caggaaagtg ggcagtgttt
    60061 gctcaataac accacctgtt gactgcaacc tgacgacttt gttatgtaca acataatttt
    60121 cattccagga aacatcatac ggtgccaggg actctgtcgg gcagtcagag ctaacttttg
    60181 ttggtcctat aaaaattccg gccccgaaac gctgatccat agtactggcg ctttcaacat
    60241 aagacgacac aatccaggga caaccgtgta aatctccatc gtaataaacg ccaagtacta
    60301 attcattaca tgaccaggcc gttactggag agagagcatc atactcttct aatccggggc
    60361 aggtagaatc cggtaaggtt gtattattgc gctccatcct gagttcaggg ccgggaggac
    60421 tcccggtcca actaactaaa ggcaaacttg ctgccgcagt tgaattagat tcatacgtgc
    60481 catattcaac tgattgaggg gtcaggactg aaagatgaaa acgatacttt gcaacaacgc
    60541 tggtcgtccc cgcgggtaca tagagtgtgg cgatctttgt cgcagcctgt atcccagaag
    60601 cgcacagagc cagcaccaat aaacatataa catgacgtat tttatgcatt ttatatatac
    60661 tcaaacgtag ctgtcaggct ggcggtgaaa tcaccgatgg ttacatcacg accgcttttc
    60721 gcctggatcc aggcccggaa attaatatca ttgttgccgt tggtaagcgc aaaagtcgcc
    60781 ccggtggtgt tgttaatatc cacccgcttg ccgtcgagcg tttccatccc tatccccacg
    60841 ccctgcgcgg tggaggtgtc gtcgactgcc agaaaacccg gctgaccgct gtcttccgtt
    60901 ccggtgaggg tgactttgac gttgtatccc gccggccctt tgcagtcttt cagcttaaac
    60961 acaaccggca gatgcgcgga ctgcccggcg accatcaaat cccggttgct gacggtggga
    61021 aaatgaattt ccgccagatt ggcaccatcc accaccagcg tgcaggattt actcagcagg
    61081 ttgccgtaaa aatgcaggtt gttatcgact gcccgtgccc ccgtgctggc ggtcagcagc
    61141 aggcctgcta acagcgcata accgggtgaa taaatgtgtg ctgttttcat tggtaatcca
    61201 cccgtaaggt tgcgtaagca gaaaaattcg tgtccgtcag caccgcatca gccgctttga
    61261 ccggtaccgc ctccagcttt ggcttctggc taaaatcggt ggcgttaatg gaaagcggct
    61321 cgttgagctt aaaacgctga ccgtcatgct gaagctcaat tccgaagccc ggtacatccg
    61381 tctcaatagc agagtcgttg taggaggtgg cagtccccgt ccaggagagt gtcatattcc
    61441 actcgggatc gcggacatcc gagtcgcagc tcagttcgta gtccactttc tgacgaccat
    61501 aattgccatc aatgctgtcg ataataagat cgcgaaattg cacttcgatg gtttgcccgc
    61561 cgctgatgct acaggcgggc ggcgacatca gcgtaccgtg gaatgtgatg tcatcttccg
    61621 ccgccagcac agccccgcaa ccagcgcaca gtaaaaggag caggaatttt ttcattggta
    61681 atccaccacc atggtcatcg tggcgttaaa ctccgacggc gtcagttgca ccccgctctg
    61741 ttttaccggc accgctttca gctgaggctg agtgttgaca ttaaaaggcg tccagttatt
    61801 ttcaccaact gcaaacaggg agttgtcggc ggcgttttct atccgcaccc ccaggccggc
    61861 gatatccgtg ctgagaaccg tttctccgtt gatcacggcg gtattgcctt tgagctgcat
    61921 ccgtaagtca tcgctgactt tattaccgca gttgagggtg tatttcgcat cctgttggta
    61981 attggtacca tcgatcctgt tgattatcac attgccaaat ttcacagcgc ttcccttcac
    62041 ggagcaaggc ggcggtccat cgaccaggat tcgcagcgtc agatccacct gtttgatatc
    62101 actgccagca acagccaaac cgctgatgcc ccatccggcc agcaccagca accctgcgcc
    62161 cgctacaaac ttgtgactca taagtccctc ccggcatcag cctttgcggc tctgctcttc
    62221 atccacctgg cagctgttgc cggtgcatat gaagtacagc ggcatccgcg cgccgtagtc
    62281 gttgatgtaa gtcagcaccg gcacgctgtc catcttcgca ttcagcggtg cggaggtttt
    62341 cggctccatc atcagcggtg tgaatcccgc cggcgggttg ccgccttttt tagcgctggc
    62401 gttgctgatg atcacgtagt acggcgtcgg gttattgacg gtgaagtcct ggccgttacg
    62461 gatgagcgtc accttatgct gccacggact tttcatgctg gtgttctcca gcgccttcgg
    62521 tcgccagaac agtttgatac gcgtctgcag cgcaatctgc agtgtgttcg gtttatttga
    62581 tttcggcgga atttcgcgca cgttgaaata gaacacgctc tcgcggtcgg ccggcagttt
    62641 gttgatatcc ggcagcccct gcaccttcac ctggccgttc atcatcgcat cgatacgctg
    62701 cactggcgga agcaccgcaa gcggcgagct gattttgttg ccctttgcat cctctatcca
    62761 gctctgcgcc agataaggca gtgtgggatc gttgttgcgc agcgtgacgc tgatggactt
    62821 gtcgctttcg ttaaacacca gccgtgtcct gtcgggcgtc accgaagcgt ggctggagaa
    62881 actgaccact gtcgccagca gcgccagtgc cgggacaacg atttttgcag tgttagtcag
    62941 attaaacatg gatcattcct taatcacgtt actggtttac tgaaattgaa gtcggaggtt
    63001 ccgttggcgt cacccgctgt gtctgttcct gaatcgccgg tttgaccgcc ggcgaagtga
    63061 taggtgctac attgccttta tgctggcatg gcagtaacaa gccgttaaac agatcggcag
    63121 gcagcgggtc cggcaggttg atatcgcaat gcgcaacacc actccagaac acactcatgt
    63181 gttcgccagg tttcacccca gccagataaa cattgccgtc atcatcgaca aggcccactg
    63241 tctgctcgtt atcatttttt acttctgcgc caaacggagg atggctgccg tcgctcaggc
    63301 gcagcaccgc catcgctttt tgtccgctaa tgacagcaaa tttacggtag ccaatggcac
    63361 cttcagttag cgtggcttgc accaccgact gggttgcttc agcgttttca ggcaatctgt
    63421 tgaggtcgat atacgcctga ttgcggtaat agttattgac gtcagaaacg acggctttac
    63481 caaacatatt ggtataaaca gcagccccgt taccttccac cggaacatcg gcaacgccat
    63541 cggcatcaat caacaatcgt gtcccgccca tattctgggt acggtgaagt gcgccaccgt
    63601 gggcagtaag cgttgcgccg ccctgtaacg acaagcccgc agaggtgtat tgcccttcat
    63661 ggtagttcgc actgaggtca acctgcgcca gcgaaccatc atggctgtaa tagccgtcaa
    63721 cgctggtgtg tttgtcactg gtgccgacgt tcaactgata gtgagtcgcg tcatcgacac
    63781 ggctgaaata accgacctga ctgctgtccg tcccactgcc atagttaccg ttatagctaa
    63841 cggtactgtt gtcgccccac ggcatactga gcgaaatgta catgcctttg tcggcctggt
    63901 tgtcatactc gtaacggtag ccagtcatcg agatgctgac attacgaata ctgcccatat
    63961 tgaaatagtg cgagagcatg atgttgtagt ttgtctgctc ctcgcgatcc cagtaggtat
    64021 ggcgggtata gttgagataa accgaaacac ccacatcgcg gaagttctgg ttataagtgg
    64081 cggtgtacat ctctttgtcg ttgcccgtgc gtaccattcc gctgtcactg gcatccagat
    64141 actcgctcat ggtcataaag ttctcttccg agaagcgata tccagcaaaa gtaacgcggc
    64201 tgttgagctg gtcaaagtct ttggaataac tcacacggaa ggagttaccg tccagcgaac
    64261 ctttgccata agcggtgtct ttatccagtt tggtgtgcga gtgagtaaca tcaaacgcga
    64321 ccgcgccgaa tgtagacaaa tcgcgaccga cgccaagcgc cgcagactga tagttttcat
    64381 ctcccagtgc gccgccatat aacgaccagc cgttagcgat cccccaggaa gcttcagcac
    64441 cagaaaaaaa ttcaccctcg atatggtgtc cccactcttg cggacggccc atcatgatct
    64501 tatagcgaac ctgacctggg cgagtgaggt atggcatcga ggcggtgctg atgtcatatt
    64561 cctgcacctg gccgttctgt tcttcaatgc gaatatgcaa cgtaccggag acagaatcac
    64621 caagatcctg aatacgaaac ggtccagccg gaacctgcgt ttcgtaaatc acacgcccca
    64681 tctggctgac ggtcactttt gctgtggtgt gtgccacgcc ggaaatgtct ggcgcgtagc
    64741 cgcgcagatt gggaggcaac atttgatcgt cagtactgac actgccacca acatagttaa
    64801 agccatcaaa aatatcggat ctgaggtaat cctcgcccag cgccagtttg gcttttaatg
    64861 atggtaacgc ccgccaggca tagtagcgac tccactccca ttttttttga gtttcatcgc
    64921 cgctgaattc atcgtcatca tcattactgc gagtatgttg atagttagtc tgccagtcag
    64981 cacgcatacg ccacggcccc aggttaaccc cgaccgtccc gttgccgctg atctcgttac
    65041 tatcatcacc gccattttct tcgtgccggg tttgtgcgtt gatgctgtag tccgcgacga
    65101 tcccggagat gccgtcatcc caacgtgaag gcggatccca gtcgggataa gtatattcga
    65161 ggtaagcctg tggcagtgaa atgactaatg cggactggct taaatcagcc ttaatttcca
    65221 tgccttccag ttgaccggat ttcaggcatt tagcatcgtg gctccattgc agatttttcg
    65281 ccacgtcttc ttttaaacca aactgcgcta ccagttccgg tgtcagacaa gcatagcttt
    65341 tgctcgcgtc atcttcacca gcataccagt aaatatcgta ctcttccgcc aatggctgtt
    65401 tatttagttg aacctgtaaa ttgtattttc cgggctcaac atatccctgg ctggaaaaac
    65461 gcttcagatc aatttttgtg tcgcctttta attccagaaa acgggaatca aactgaatgt
    65521 cgtcttcagc ccagacactg ctataactcc ctgacattgc cagcgcaatg caccacggaa
    65581 gaatccgtaa tcgaaaaaga gaatggtcag gcatacacgt ttctatccgt ttttattctt
    65641 gttcggtcaa aatccattta aatcccccat agcagtgtcg ctatgggggt atgccttaca
    65701 ggtaagtaat ctggaaggtg gtatttgctt cgaaattacc cagatctggc gcatcagctg
    65761 cgccgaccag ccaggcttta aagttcagtg tctgcttcgc tttgccttta ttggtagaag
    65821 tgtctttcac tattttctgg tcaataccca tgccgctttt gtaagaggta cccagagagt
    65881 caccgatcgc caggctaaca ttgttaaatg ccgcaccggt atcggtattg aaaatggtgt
    65941 aataattgcc gctatttgca gaagaaacgg taccagtaaa ggtagtggtc attgtttcct
    66001 gagtatcaaa tacgcaatct tgcagacgaa tctggaaact tttcggcgtg gtggtgccgt
    66061 tatttttcag tttttcggca ccgatatcac ccatatcaac ttccagtttg tgatcgtccg
    66121 gaacgataga acacactgag tcttcaatgg tgccgtaaaa acgtgcagta ccgttgttac
    66181 ccgcagcgat tgttgccgta gaggtgaaaa cgcccatcac cattgccgca gcaatagctg
    66241 ttttaacaaa
//
LOCUS       DAZGJW010000007        65639 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZGJW010000007 DAZGJW010000000
VERSION     DAZGJW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.007"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            130..648
                     /locus_tag="ACHKIK_000481"
     CDS             130..648
                     /locus_tag="ACHKIK_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO9081719.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            664..1203
                     /locus_tag="ACHKIK_000482"
     CDS             664..1203
                     /locus_tag="ACHKIK_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO9081720.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKRVGKAEENKSDSWFN"
     gene            complement(1298..2875)
                     /gene="guaA"
                     /locus_tag="ACHKIK_000483"
     CDS             complement(1298..2875)
                     /gene="guaA"
                     /locus_tag="ACHKIK_000483"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO9081721.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2944..4410)
                     /gene="guaB"
                     /locus_tag="ACHKIK_000484"
     CDS             complement(2944..4410)
                     /gene="guaB"
                     /locus_tag="ACHKIK_000484"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO9081722.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4572..5942
                     /gene="xseA"
                     /locus_tag="ACHKIK_000485"
     CDS             4572..5942
                     /gene="xseA"
                     /locus_tag="ACHKIK_000485"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO9081723.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQTVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5939..6154)
                     /locus_tag="ACHKIK_000486"
     CDS             complement(5939..6154)
                     /locus_tag="ACHKIK_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO9081724.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6223..7695)
                     /gene="der"
                     /locus_tag="ACHKIK_000487"
     CDS             complement(6223..7695)
                     /gene="der"
                     /locus_tag="ACHKIK_000487"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO9081725.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7813..8991)
                     /gene="bamB"
                     /locus_tag="ACHKIK_000488"
     CDS             complement(7813..8991)
                     /gene="bamB"
                     /locus_tag="ACHKIK_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO9081726.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGLTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(9002..9622)
                     /gene="yfgM"
                     /locus_tag="ACHKIK_000489"
     CDS             complement(9002..9622)
                     /gene="yfgM"
                     /locus_tag="ACHKIK_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO9081727.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9640..10914)
                     /gene="hisS"
                     /locus_tag="ACHKIK_000490"
     CDS             complement(9640..10914)
                     /gene="hisS"
                     /locus_tag="ACHKIK_000490"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO9081728.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(11025..12143)
                     /gene="ispG"
                     /locus_tag="ACHKIK_000491"
                     /gene_synonym="gcpE"
     CDS             complement(11025..12143)
                     /gene="ispG"
                     /locus_tag="ACHKIK_000491"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO9081729.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12170..13183)
                     /gene="rodZ"
                     /locus_tag="ACHKIK_000492"
     CDS             complement(12170..13183)
                     /gene="rodZ"
                     /locus_tag="ACHKIK_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO9081730.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13468..14622)
                     /locus_tag="ACHKIK_000493"
     CDS             complement(13468..14622)
                     /locus_tag="ACHKIK_000493"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO9081731.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14772..15203)
                     /gene="ndk"
                     /locus_tag="ACHKIK_000494"
     CDS             complement(14772..15203)
                     /gene="ndk"
                     /locus_tag="ACHKIK_000494"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO9081732.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15352..17664)
                     /gene="pbpC"
                     /locus_tag="ACHKIK_000495"
     CDS             complement(15352..17664)
                     /gene="pbpC"
                     /locus_tag="ACHKIK_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO9081733.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGAWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NANRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     SYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17665..22626)
                     /gene="yfhM"
                     /locus_tag="ACHKIK_000496"
     CDS             complement(17665..22626)
                     /gene="yfhM"
                     /locus_tag="ACHKIK_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO9081734.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPSETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNHHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRVDASGYP
                     QSAPLPANDVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22833..23678
                     /gene="sseA"
                     /locus_tag="ACHKIK_000497"
     CDS             22833..23678
                     /gene="sseA"
                     /locus_tag="ACHKIK_000497"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO9081735.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            24014..24139
                     /gene="timP"
                     /locus_tag="ACHKIK_000498"
     CDS             24014..24139
                     /gene="timP"
                     /locus_tag="ACHKIK_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO9081736.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24171..24947)
                     /gene="sseB"
                     /locus_tag="ACHKIK_000499"
     CDS             complement(24171..24947)
                     /gene="sseB"
                     /locus_tag="ACHKIK_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO9081737.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPGFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGGEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25089..26372)
                     /gene="pepB"
                     /locus_tag="ACHKIK_000500"
     CDS             complement(25089..26372)
                     /gene="pepB"
                     /locus_tag="ACHKIK_000500"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO9081738.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26550..26750)
                     /gene="iscX"
                     /locus_tag="ACHKIK_000501"
     CDS             complement(26550..26750)
                     /gene="iscX"
                     /locus_tag="ACHKIK_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO9081739.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26762..27097)
                     /gene="fdx"
                     /locus_tag="ACHKIK_000502"
     CDS             complement(26762..27097)
                     /gene="fdx"
                     /locus_tag="ACHKIK_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO9081740.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27099..28949)
                     /gene="hscA"
                     /locus_tag="ACHKIK_000503"
     CDS             complement(27099..28949)
                     /gene="hscA"
                     /locus_tag="ACHKIK_000503"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO9081741.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28966..29481)
                     /gene="hscB"
                     /locus_tag="ACHKIK_000504"
     CDS             complement(28966..29481)
                     /gene="hscB"
                     /locus_tag="ACHKIK_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO9081742.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29577..29900)
                     /gene="iscA"
                     /locus_tag="ACHKIK_000505"
     CDS             complement(29577..29900)
                     /gene="iscA"
                     /locus_tag="ACHKIK_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO9081743.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29917..30303)
                     /gene="iscU"
                     /locus_tag="ACHKIK_000506"
     CDS             complement(29917..30303)
                     /gene="iscU"
                     /locus_tag="ACHKIK_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO9081744.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30331..31545)
                     /gene="iscS"
                     /locus_tag="ACHKIK_000507"
     CDS             complement(30331..31545)
                     /gene="iscS"
                     /locus_tag="ACHKIK_000507"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO9081745.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31786..32274)
                     /gene="iscR"
                     /locus_tag="ACHKIK_000508"
     CDS             complement(31786..32274)
                     /gene="iscR"
                     /locus_tag="ACHKIK_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO9081746.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32575..33312)
                     /gene="trmJ"
                     /locus_tag="ACHKIK_000509"
     CDS             complement(32575..33312)
                     /gene="trmJ"
                     /locus_tag="ACHKIK_000509"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO9081747.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAAQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33431..34234
                     /gene="suhB"
                     /locus_tag="ACHKIK_000510"
     CDS             33431..34234
                     /gene="suhB"
                     /locus_tag="ACHKIK_000510"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO9081748.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34379..35233
                     /locus_tag="ACHKIK_000511"
     CDS             34379..35233
                     /locus_tag="ACHKIK_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9081749.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35424..36704
                     /gene="csiE"
                     /locus_tag="ACHKIK_000512"
     CDS             35424..36704
                     /gene="csiE"
                     /locus_tag="ACHKIK_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO9081750.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36696..37835)
                     /locus_tag="ACHKIK_000513"
     CDS             complement(36696..37835)
                     /locus_tag="ACHKIK_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO9081751.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37995..38885)
                     /gene="hcaR"
                     /locus_tag="ACHKIK_000514"
     CDS             complement(37995..38885)
                     /gene="hcaR"
                     /locus_tag="ACHKIK_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO9081752.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPVYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            39021..40382
                     /gene="hcaE"
                     /locus_tag="ACHKIK_000515"
     CDS             39021..40382
                     /gene="hcaE"
                     /locus_tag="ACHKIK_000515"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO9081753.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40379..40897
                     /gene="hcaF"
                     /locus_tag="ACHKIK_000516"
     CDS             40379..40897
                     /gene="hcaF"
                     /locus_tag="ACHKIK_000516"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO9081754.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40897..41217
                     /locus_tag="ACHKIK_000517"
     CDS             40897..41217
                     /locus_tag="ACHKIK_000517"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO9081755.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41214..42026
                     /gene="hcaB"
                     /locus_tag="ACHKIK_000518"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41214..42026
                     /gene="hcaB"
                     /locus_tag="ACHKIK_000518"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO9081756.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            42036..43238
                     /gene="hcaD"
                     /locus_tag="ACHKIK_000519"
     CDS             42036..43238
                     /gene="hcaD"
                     /locus_tag="ACHKIK_000519"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO9081757.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43335..43757
                     /locus_tag="ACHKIK_000520"
     CDS             43335..43757
                     /locus_tag="ACHKIK_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO9081758.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43805..44677)
                     /locus_tag="ACHKIK_000521"
     CDS             complement(43805..44677)
                     /locus_tag="ACHKIK_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO9081759.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44689..45783)
                     /locus_tag="ACHKIK_000522"
     CDS             complement(44689..45783)
                     /locus_tag="ACHKIK_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO9081760.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45816..46814)
                     /locus_tag="ACHKIK_000523"
     CDS             complement(45816..46814)
                     /locus_tag="ACHKIK_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9081761.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46839..48350)
                     /locus_tag="ACHKIK_000524"
     CDS             complement(46839..48350)
                     /locus_tag="ACHKIK_000524"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO9081762.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIIAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(48373..49356)
                     /locus_tag="ACHKIK_000525"
     CDS             complement(48373..49356)
                     /locus_tag="ACHKIK_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO9081763.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGGDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49453..52734)
                     /locus_tag="ACHKIK_000526"
     CDS             complement(49453..52734)
                     /locus_tag="ACHKIK_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO9081764.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNQEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52852..54045
                     /locus_tag="ACHKIK_000527"
     CDS             52852..54045
                     /locus_tag="ACHKIK_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO9081765.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVSTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(54109..55362)
                     /gene="glyA"
                     /locus_tag="ACHKIK_000528"
     CDS             complement(54109..55362)
                     /gene="glyA"
                     /locus_tag="ACHKIK_000528"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO9081766.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55690..56880
                     /gene="hmpA"
                     /locus_tag="ACHKIK_000529"
     CDS             55690..56880
                     /gene="hmpA"
                     /locus_tag="ACHKIK_000529"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO9081767.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56925..57263)
                     /gene="glnB"
                     /locus_tag="ACHKIK_000530"
     CDS             complement(56925..57263)
                     /gene="glnB"
                     /locus_tag="ACHKIK_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO9081768.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57324..58658)
                     /gene="glrR"
                     /locus_tag="ACHKIK_000531"
     CDS             complement(57324..58658)
                     /gene="glrR"
                     /locus_tag="ACHKIK_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO9081769.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58648..59361)
                     /gene="qseG"
                     /locus_tag="ACHKIK_000532"
     CDS             complement(58648..59361)
                     /gene="qseG"
                     /locus_tag="ACHKIK_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO9081770.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(59526..60953)
                     /gene="qseE"
                     /locus_tag="ACHKIK_000533"
     CDS             complement(59526..60953)
                     /gene="qseE"
                     /locus_tag="ACHKIK_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO9081771.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCICRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61529..65416)
                     /gene="purL"
                     /locus_tag="ACHKIK_000534"
                     /gene_synonym="purI"
     CDS             complement(61529..65416)
                     /gene="purL"
                     /locus_tag="ACHKIK_000534"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO9081772.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGLGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIGSLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
BASE COUNT        15465 a        18189 c        16563 g        15422 t
ORIGIN      
        1 tatttttata aattattccc tgcgtgaatt ttaataaatt taatctatcc ctttatacgc
       61 aatacattta ctttcctctt ttgatgatct taaatgtctt atttttcgta atgtgtataa
      121 caaggaatag tgatgaaatt taaaaaatgt cttctgcctg tggcaatgtt agcgtcattc
      181 actctggcag gatgccagtc aaatgctgac gatcatgccg ccgatgttta tcaaaccgat
      241 caactgaata ccaaacaaga aactaaaacc gttaatatta tttccattct tcccgcaaaa
      301 gttgccgtag acaactccca aaataaacgg aacgcacaag ccttcggcgc gcttattggc
      361 gcagtcgctg gcggtgttat cggccacaac gtcggttctg gcagcaattc cggaacgacg
      421 gcaggggcag ttggcggcgg agctgtaggc gcggcagtgg gttctatggt gaatgataaa
      481 accttagtgg aaggtgtttc tttaacatat aaggaaggca ccaaagtgta tacctctacc
      541 caggtgggta aagagtgcca gtttacgaca ggtttagccg ttgttattac cacaacgtat
      601 aacgaaacgc gtattcagcc aaatactaaa tgtcctgaaa agagctaata atcaggagga
      661 gtcatgaaga aagtttttct ttgcgccatc ttggcctcct taagctatcc ggctatcgcc
      721 tcatcattgc aggatcaact ctctgctgta gcagaagctg aacagcaagg taaaaatgaa
      781 gagcaaaggc agcatgacga atgggtcgcg gagcgcaaca gggaaatcca gcaagagaag
      841 caacgtcgcg caaatgccca ggccgccgct aacaaaagag cggcaacggc agcggcaaat
      901 aagaaagctc gtcaggataa actggacgcc gaagcctctg cggacaaaaa acgcgatcaa
      961 agttatgaag atgagctacg cagcttagag attcagaaac aaaaactggc gctggcaaaa
     1021 gaagaagccc gcgttaagcg agaaaacgaa tttatcgatc aggaactgaa gcacaaagct
     1081 gcgcaaaccg atgtggtgca atctgaagct gacgccaaca gaaatatgac tgaaggcggt
     1141 cgcgatctga tgaaacgcgt gggcaaagca gaagagaaca aatcggacag ctggtttaat
     1201 taatcgatgt tagtaacttc aagcctatga ttcttgaaga taaaaaaccc tctgtagtaa
     1261 cagagggttt tgttcattca tagtgcaggg attaaaatca ttcccactca atggtagctg
     1321 gcggcttgcc gctgatgtca tacaccacgc gggaaatacc gttcacttca ttgataatgc
     1381 ggttggaaac gcgaccgagg aaatcgtacg gcagatgcgc ccagtgtgcg gtcataaagt
     1441 cgatggtttc gacagcacgc agagagacaa cccaatcata cttacgacca tcgcccatta
     1501 cgccaacgga acgtaccggc aggaacacag tgaacgcctg gctgactttg tcgtacaggt
     1561 ccgctttacg cagttcttca atgaagatgg cgtcagcacg gcgcagcagg tcacagtact
     1621 ctttcttcac ttcacccagc acacgaacgc caaggcctgg tcccgggaac gggtgacggt
     1681 acagcatgtc gtacggcagg cccagctcca gaccaatctt acgcacttcg tctttgaaca
     1741 gctctttcag cggttcaacc aggcccatct tcatctcttt cggcaggccg cccacgttgt
     1801 ggtgagattt gatgacgtgt gctttaccgg ttgcagaagc cgcagattcg ataacgtcag
     1861 ggtagatggt gccctgcgcc agccacttca cgtcttccag tttcagcgct tcttcatcga
     1921 atacttcaac gaaaacgcgc ccgatgattt tacgttttgc ttccggatcg ttttcgccag
     1981 ccagcgctga caggaagcga tcttctgccg gtacgtgaac aatgttaaga ccaaagtgat
     2041 cgccaaacat atccagaacc tgctctgctt cgttgagacg cagcaggccg ttgtcgacga
     2101 atacgcaagt caggttttta ccgatagcgc ggtgcagcag cattgcggtt acggaggaat
     2161 ccacaccacc agagaggccg aggatgactt tatcgtcgcc tacctgctcg cggatgcgag
     2221 ctacagcatc gtcgataatt ttcgctggcg tccacagggc ttcacactgg cagatatcac
     2281 gcacaaaacg ctccagcatg cgcataccct ggcgggtgtg agtcacttcc gggtggaact
     2341 gtacgccata gaagcgtttt tcttcgttag ccataatggc aaacgggcag ctttcggtgc
     2401 tggctacggt gacgaagtcg gacgggatag cggtaacttt atcgccgtgg ctcatccaga
     2461 catcgagcag cggtttaccg tctgcggtca gcgcatcttc gataccgcga accagtgcgc
     2521 tgtcgtttac gacttcaacc tgcgcgtagc caaattcacg ttcgttagag gcttcaacgt
     2581 gaccgcccaa ctgcattgcc atggtctgca tgccatagca aacgccgaat accggtacgc
     2641 ctgcttcaaa gacatactgc ggcgcacgcg ggctgttttc ttcggtggtg ctttccgggc
     2701 cgccggaaag aataatgccg cttggattga agtcacgaat ttgtgcttct gtcacatccc
     2761 acgcccacag ttcgcagtaa acacccagct cacgcacgcg gcgcgcaacc agttgagtgt
     2821 actgagaacc gaagtccaga atgaggatgc gatgcttatg aatgttttcc gtcattgacg
     2881 cttattccga ggcaagtgaa acagataata taaatcgccc gacatgaagt cgggcgaaga
     2941 gaatcaggag cccagacggt agttcgggga ctctttagta atggtcacgt cgtgaacgtg
     3001 gctttcctga atgcccgcac cgctgatacg tacaaactcc gctttagtac gcagttcgtc
     3061 gatagtacca cagccggtca gacccataca ggagcgcagg ccgcccatct gctggtgaat
     3121 gatctctttc aggcgacctt tataagctac gcgaccttcg ataccttccg gcaccagttt
     3181 gtcggcagcg ttatcgctct ggaaataacg gtcagaggaa cctttggaca tcgcgcccag
     3241 ggaacccata ccacggtaag atttgtaaga acggccctgg tagagttcga tttcacccgg
     3301 agattcttca gtacccgcca gcatggaacc taccatcacc gcgcttgcgc cagctgcgat
     3361 agctttggcg atgtcgccgg agaagcgaat accaccatct gcgataaccg gaataccggt
     3421 gccttccagg gcttcaactg cgtcagcaac ggcagtgatc tgcggaacac ctacgccagt
     3481 aacgatacga gtcgtacaga tagaaccagg gccgataccg actttaaccg cgctgcaacc
     3541 agcttctgcc aaagcgcgtg cacctgcagc tgttgccacg ttgccgccga tgatttgcag
     3601 atccggatat ttagcacggg tttcacggat acgctgcaaa acgccttctg agtgaccgtg
     3661 ggaggagtcg atcagcagaa cgtcaacgcc agcggcaacc agcgcgtcaa cacgctcttc
     3721 gttacccgca cctgcgccaa ctgcagcacc aacacgcaga cggccttgct cgtctttaca
     3781 ggcgttcggt ttacgttccg ctttctggaa gtctttcacg gtgatcatgc cgatcaggtg
     3841 gaattcgtca tcaaccacca gcgctttttc aacgcgtttt tcgtgcattt ttgccagcac
     3901 cacttcacgg gcttcacctt cacgcacggt gaccagacgc tctttcggcg tcatgtaaac
     3961 gctaaccggc tggttcaggt cggtaacaaa acgcacgtca cgaccggtga taatacccac
     4021 cagttcgttt tcttcggtaa cgaccggata gcccgcaaaa ccgttacgct cggtcagttc
     4081 tttcacttcg cgcagcgtcg tggttggcag aacagtctgc ggatcagtca ccacaccaga
     4141 ttcgtgtttt ttcacacgac gaacttcttc tgcctggcgt tcaatggaca tgtttttgtg
     4201 gataaagccg ataccgcctt cctgagccag agcaatagcc aggcgcgctt ccgttacggt
     4261 atccattgct gcggaaagca tagggatatt cagacgaata gttttcgtca gctgggtgct
     4321 gaggtcagca gtattcggca gaacggtaga atgagcagga acgaggagaa cgtcgtcaaa
     4381 cgtcagagct tctttagcga tacgtagcat gggcaatatc tcgaccagag tggttaataa
     4441 atattgccgc ggcattatac agagcgtaac cgattgcatc tacccctttt tgcaaaaaat
     4501 gcttgctatc cccgaagggc gggttactat cgactgaata acctgctgat ttagaatttg
     4561 atctcgctca catgttacct tctcaatccc ctgcaatttt taccgttagt cgcctgaatc
     4621 aaacggttcg tctgctgctt gagcatgaga tgggacaggt ttggatcagc ggcgaaatct
     4681 ctaatttcac acaaccggct tccggtcact ggtactttac actcaaagac gacaccgccc
     4741 aggtacgctg cgcgatgttc cgcaacagca accgtcgggt gaccttccgt ccacagcatg
     4801 ggcaacaagt tttagttcgc gccaatatta cgctctacga gccgcgcggc gactaccaga
     4861 taatcgttga gagtatgcag ccggccggtg aagggctgct gcaacagaag tacgaacagc
     4921 tcaaagcgaa gttgcaggct gaaggattgt tcgatctgca atacaaaaaa ccacttccct
     4981 cccctgcgca ttgcgttggt gtgatcacct caaaaaccgg tgctgcgcta catgatattt
     5041 tgcatgtgtt aaaacgtcgc gatccttctc taccggtgat catctacccc accgccgttc
     5101 agggcgatga cgcgccgggg caaactgttc gcgccattga actggcgaat cagcgcaatg
     5161 agtgcgacgt gttgatcgtt gggcgcggcg gcggttcgct ggaagattta tggagtttta
     5221 acgacgaacg cgtagcgcgg gcgatttttg ccagccgcat tccggtcgtc agcgccgtcg
     5281 ggcatgagac ggatgtgacc attgccgatt ttgttgccga tctgcgtgcg ccaacgccgt
     5341 ctgccgccgc tgaagtagtg agccgtaatc agcaagagtt actgcgccag gtgcaatcga
     5401 cccgtcaacg gctggagatg gcgatggatt attatctcgc caaccgcacg cgtcgcttta
     5461 cgcagatcca tcaccgatta cagcaacagc atccgcagct ccggctggca cgccagcaaa
     5521 ccatgcttga gcgcctgcaa aagcggatga gctttgcgct ggaaaatcaa cttaagcgta
     5581 ccgggcaaca gcagcagcgg ttaacacagc ggctgaatca gcaaaatcca cagccgaaga
     5641 ttcatcgcgc gcaaacgcgc attcagcaac tggaatatcg tttagcagaa accctgcgcg
     5701 tacagcttag cgccacgcgt gaacgtttcg gtaatgcagt aacgcacctc gaagccgtaa
     5761 gcccactgtc aacgctggcg cgtggataca gcgttactac tgctactgac ggcaatgtac
     5821 tgaaaaaagt gaagcaagtt aaagcgggtg aaatgctaac cacacgtctg gaagacggct
     5881 ggatagaaag tgaagtaaaa aacatccagc cggtaaaaaa atcgcgtaaa aaggtgcatt
     5941 aagccagcac gaattcaacg cgttttttcg atatcaagcc atgcccgtgc tgacagaagt
     6001 aatctactgc accgcaggct tttaacacct gcaacggttg atggcagtct ggacaaagag
     6061 ctttcatttc aataatttcc ccgcaggatg ggcaacgcgc atggtcgtta tcctgatcaa
     6121 gcacatgctg acattgtgga caatgcagtt ccatatgaat tcctccggta gccatccctc
     6181 tacattcata gagggaatgg cagataaaat acttacggat aattatttat ttttcttgat
     6241 gtgcttcatc agacgcttac gtttacgcat ctgggttggc gtcagggtgt tacgcttgtt
     6301 cgcatacggg ttttcccctt ctttgaactg aatacgaatc ggcgatccca ttacgtccag
     6361 cgatttgcgg aagtagttca tcaagtagcg cttgtaggaa tcaggcaggt ctttcacctg
     6421 attaccgtga atcaccacaa tcggcgggtt ataaccacca gcgtgggcat atttcagctt
     6481 cacacgacga ccgcgtacca gcggcggttg gtgatcttca acagccatcg tcatgatgcg
     6541 cgtcagcata gaggtcccca cacgacgggt ggagctgtca tacgcttcac gtactgattc
     6601 aaacaagtta ccaacaccac tgccgtgcaa ggcagagata aagtgcacac gagcaaaatc
     6661 gataaagccc agacggaagt ccagcgtttc tttcacctgc tctttcactt cctggctcag
     6721 gccatcccac ttattcacca caatgacaag tgagcgccca ctattgagaa taaagcccag
     6781 cagcgagaga tcctgatcgg aaataccttc gcgcgcatca atcactaaca tcaccacgtt
     6841 ggcatcttca atggcctgca acgttttgat tacggagaat ttctctacag catcggtgat
     6901 tttgccgcgt ttacgtacgc cagcggtgtc aatgagcaca tactcacgtc catcgcgttc
     6961 cattggaatg tagatactgt cacgcgtcgt gccaggcatg tcataaacaa caacgcgctc
     7021 ttcaccaaga atacggttag tgagtgtaga cttacctacg ttcggacgac ccacaatcgc
     7081 cagtttgatc ggcagacttt gcgggtcgaa gtcgtcttcc tcttcttctt cgccgttctc
     7141 ttccgcttca aattgcgccc agtattcagc gtcttcgtcg acttcctctt gcggtgcgag
     7201 atcttccatc cacggcagca gcacatgctc cagcagactt aatacgccac gaccgtgaga
     7261 cgcggcgatc gggtagattt cacctaaacc aagcgagtag aaatcaacca ctgcctgatc
     7321 gggatccaga ccgtcagttt tgtttgccac caggaaggtc ggtttttcac gggagcgcag
     7381 atgtttggca atcgcttcat ctgccggcat caggcccgcg cgcgcatcca ccataaacag
     7441 tacgacgtcc gcttcttcaa tcgccagcag cgactgttcc gccatgcggg tttctacacc
     7501 gtcttctgtg ccatcaatcc cgccggtatc aatacagata aactcacggc cttcaatttc
     7561 cgcacgaccg tacttacggt cacgagtcag acccgggaaa tccgcaacca gcgcatctcg
     7621 ggtgcgagtt agacggttaa ataacgtgga ttttcctacg ttagggcgcc cgacaagcgc
     7681 gaccacaggt accatgttta aagcctcatt tttataaatc atcagacaac gcacgctata
     7741 ttcgcgtcgt tgttaaaaac aggaaaacgg cccctgtcca ggagccgttt tcaaagtgaa
     7801 cgacagagac gattaacgtg taatagagta cacggttccg tcttttgcct ggatcagcag
     7861 tttgccgtca gcggcaaccg gttcagtctg gaaaccggaa ctatcaactt tttgctgggc
     7921 aacgaaacga ccatcttcga cgttaatcca gtgcagataa ccttcactgt cacctaccac
     7981 caggttgcca ttatacagca ccggagaagt cagcaggcga tgcaacagat cgctttgtgt
     8041 ccacagcgta acgccgccat caatggtcaa cgccatcacc cggtcatttt gatcgaccag
     8101 atagatgcga ttgccgtcga cgatgaaatc attcaccgaa cccagttcgc gtttccacat
     8161 aatctgacca ctgcgcagat caagcgccgt caggttacca ttataggcca gcgcgaaaac
     8221 aacgccgtta acaacgacgg gagtcgtgtc aacatcgctc agacggtcaa tttcggtaga
     8281 accggtcgcc tgggaaatac gctgctgcca aatcatctgg ccctgttcca tcagcactgc
     8341 gctgacgcga ccattatcgc cacccacgac ggccgcacca aaagccgttg ccggcgcaga
     8401 ctcgccacgc aaagagagcg aaggcatatc gaggttaact gtccatttga cagcgccgtc
     8461 agcctcgttc agcgcttgta actgaccgtt actggtgtgg attaacacca gaccgtcgct
     8521 gaccaccggg cgcgaaagtg cttcacccgc gactttagtt tgccatgcca cagtaccatc
     8581 gctggtattc agcgcgtaaa cctgcgcctt ttcgctgcca atgtagacat gcccaccaga
     8641 cacggtcaga ccgccagaaa gtaatgcagg ctctttagag aaccagccat ctttctcggc
     8701 caggctgaca gaccagattt ctttgccatc atccgcattc agcgctttta ccaaaccagc
     8761 gcggtccgct gcatagacaa cgttgtccgc cagtgccgga tgaagattgg aatagaagtt
     8821 gccaatgccg ctaccaacgg aagtgctcca cgccgtggtc ggcgtaaact ggttttcaac
     8881 ggttggcaat ggggacatct ttaccacatc ttcttcgctg ttaaacagcg aacagccgct
     8941 taaaagggta acggaaagca gtcctggcag cagtaattta cgcaattgca tcgggtccct
     9001 ctcagatgga caaattatta attttcatct gcatcatttc gctcagtgcc ggagtaacat
     9061 cgcttttcac gcctgcttcc catgcactac gcgcaccttg cttatcacct ttgctcagca
     9121 atgcttcacc acgcaggtcg gcaacaatgg cagcccaccc ttcacctttg atggtatcaa
     9181 gggttttcag cgcggcatca gcctgcttga gctgtacctg aacgcgagca agacgcagat
     9241 ttatcacggc tttgagattt tcatcgctcg tgtctgccag cccctgttgt aactgggcgg
     9301 cagctttctc tagttcattt ttgtcaacaa attgctgcgc aagttccaaa gaagccagcg
     9361 caccataagt atttttattt tcagcagcaa atttttccgc cgccgggatg ctatccggtt
     9421 tgccttcgct cactgcggta accgcatttt gataggcaag agaagcggag cgtgcagaat
     9481 caacctgatg gctgttccag tagcgccagc caatcagtgc gccaacgccc aaaatcaccc
     9541 caacagccag tgctttgcca ttttcagcaa aaaagcgttt aaccgcttct acctggtcgt
     9601 tttcgttctc gtaaatttcc acgctgtcct tctccttcct taacccagta acgtgcgcaa
     9661 atgcgcggct acgctatcct gcgcaactgc cgtttgctca ccagagcgca aatccttcac
     9721 tactgctgtg ccgttagcca cttcagactc acccagcacc acagcaacgc gggcacccca
     9781 tttatcagca cgggcaaact gtttcttaaa gttgccgccg ccgtggttgg tcatcaattt
     9841 cacgcccggt aattcatcac gcagacgctc agctaatgcc atagccgcag attgtgtatc
     9901 agcacctgaa gccaccaggt atatatcgac aacaggatcg gctttaaatt ccggattaac
     9961 ggcctgtact aacaatacaa gacgttcgag gcccatcgca aaaccgacag ccggtgttgc
    10021 acgaccgccc agttgttcca caagaccgtc ataacgaccg cctgcacaca cggtgccctg
    10081 ggagccgaga ctgttagtca cccactcgaa aacggtacgg ttatagtaat ccagaccacg
    10141 caccagacgc tggtttacgg tgtaagcgat ccccgcgctc tcaagcagtt tgcacagacc
    10201 ggcaaaatgc tcacgagatt cctcgtccag ataatcacct aatgccggag cgtcgttgag
    10261 aagcgcctgc acttccggat tttttgaatc cagcacgcgc agcgggttag tgtacatgcg
    10321 gcgtttgcag tcttcgtcca gcttttcttt atgctgctca aggaatgcca ccagcgcatc
    10381 gcggtaattg gcgcgtgctt ccagcgaacc gatagaattc agctcaagag ttacgtgttc
    10441 ggaaataccc agcgcgcgcc accagcgggc ggtgagcata atcagttcag cgtcgatatc
    10501 cggaccttgc agaccgaaaa cttcgcagcc caactgatgg aactgacgat aacgcccttt
    10561 ctgcggacgc tcgtgacgga acatcggccc gatataccac agacgctgtt cctgattgta
    10621 cagaagacca tgctcgatgc cggcgcgtac acagcccgcc gtcccttcag ggcgcagagt
    10681 caggctgtcg ccattgcgat cctcaaaggt gtacatctct ttttcaacca cgtcggtgac
    10741 ttcaccaatc gcacgtttga atagcggggt ctgctctaca atcggcaagc ggatttcact
    10801 gtaaccgtag ctgccgagca cgtttttcag tgtgccttca atgcgctgcc agatggccgt
    10861 ttcgccaggc aggtaatcgt tcatgccgcg aatggcttga atgttttttg ccacgtttat
    10921 tctctttctg aatataaaaa tgaaccctca acgcttccct caatgtttcg ggagctatgc
    10981 gggttcaatc atacacggga agcgaggcgc ttcccatcac gttattattt ttcaacctgc
    11041 tgaacgtcaa ttcgacgcgc ttcgtccagc tgactggctt tcgcacgaat gcgtgcttcc
    11101 agctggtcga tcatatcgtt gttgtccaga cggtctttgc gcacgccatc ttcatagagg
    11161 ccgcttttct tgttgccgcc ggtgacgccg agtgtagaaa ccagcgcctc acctgggcca
    11221 ttcaccacgc agccgataat cgaaacgtcc atcggagtga tgatatcttc caggcgttgc
    11281 tccagcgcgt taaccgtacc gataacatca aattcctgac gcgaacaggt cgggcaggcg
    11341 atgaagttga tcccgcgcga acggatacgc agcgatttca aaatatcgaa accgactttg
    11401 atctcttcga ccggatcggc cgccagtgat acgcgcagcg tgtcgccgat gccttcagac
    11461 agcagcagac ctaaaccaat ggcggatttt actgccccgc tgcgtgcacc accggcttcg
    11521 gtgatcccca gatgcagcgg ctgatcgatc tgttttgcca gcaaacgata agactcaaca
    11581 gcgaggaaga cgtcagacgc tttcacgctg actttgaact gatcgaagtt caggcgatcg
    11641 agatgatcaa catggcgcat ggcggattcc agcaacgcct gcggcgtcgg ttcgccatac
    11701 ttttcttgca gatctttttc cagcgatccg gcgttaacgc caatacggat cggaatgttt
    11761 ttatcgcgcg cacagtcaac caccatgcga atacgctctt cattaccgat attgccaggg
    11821 ttaatacgca gacaatcgac gccgtattcc gctactttca gtgcaatgcg atagtcgaag
    11881 tggatgtcag ccaccagcgg cacgttaacc tgctgtttga tgagtttgaa cgcttctgcc
    11941 gcgtccatcg tcggtacgga gacacggacg atatcagcgc caacgcgttc cagcgccttg
    12001 atttgattga ccgttgcttc gacgtctgtc gtacgcgtat tggtcatgga ctgtacggcg
    12061 atgggagcac catcgccaat cggcacattc ccaacgtaaa tacgtgttga ttttctacgt
    12121 tgaattggag cctggttatg catgaaaaat ctcccgcgtt acccgtctgt tactgcgccg
    12181 gtgattgttc ggcattgagg gtcagacgcg caacctggtt agttctgata aaacgactca
    12241 gatcgacagg tttcccttga tactggatct gtactgcggc tggcgcacca attttcagtt
    12301 tgtatggtgc ctggccggtt aagttcaaat taccgtcttt acgctgcata ccgctaaaca
    12361 attttttacc ggtagcatca gtaacctcca gccagcaatc ggcagtaaag ttcatcacca
    12421 gcgcattcgg atcagccgcc ggcgtagtca cgcctgcctg atcggttggc aacggcgcag
    12481 cgccatctgg cgttgttgtt gccgttggtg ccggggtcgc ggcggtatca acatttgcct
    12541 gcgaaggcga aacaaccgca ttctgttgcg gatcaacagc tggtgctggc gcagttacgg
    12601 caggtgtttg cgtgttggtt gcggtagtat ccacagacgc tggcggcgtg gacgttgtag
    12661 ccgggtctgt agttgtcgac gtatttaacg gaacactctg cccctgctcg ctattactgc
    12721 tcagttccgc cgaagattga tcggccatag tggtgatctc ttcctgctga gctttgtggt
    12781 cttgccacca ccaggcaccg ctcaggccga taaccacaaa caacaccagc caggtgaagg
    12841 tcatcagcca gccgtcacgt tttttgcggc gtttaccgag ggaaaaactc tgcataggcg
    12901 caacttttgc agcccgtagt ggagcctgct tttccagccc tggcagcagt tcttcttctg
    12961 gaatatgtac cagacgcgca taagagcgga tatatccgcg caggaatgtt gaagcaagat
    13021 cggcgggtgc cttatcttct tcaatgtcgc gtaccgtgga aaccttcagg caaagtcgct
    13081 cggcaacggc ctgctgacta agtcctagtt gttcgcgagc attacgcagg cgagcgccgg
    13141 tagtaagtgc ttcattttgg tcgtgcgtgg cttcagtatt cattcgctgc aggtacgttt
    13201 aaatgagaat tgagatgccg gtgaaccatc atgctcaccc acaccgcgaa acatccgtta
    13261 agttaaccgt tatcatacag tataagaccg tctgtgtgct cgtgacaaag cccacattca
    13321 ctaaggctaa cttactgttg catcgttaca tactgcctta aagtcagcaa aaacgcaccg
    13381 ttaatattga ccagacaatt gcaacttaat acttcattca taaaagatta cgccacggta
    13441 cataaagtaa ccgtggcgta atggctatca gaccgcttta atgtcgatgg cttcaccctg
    13501 catccgttta cgcagggtac gtttcgtacg gtcgataaca tcgcccgcca actgaccaca
    13561 ggcagcatcg atatcatcac cacgggtttt acgcacaata gtggtgaaac cgtagctcat
    13621 cagcactttt gagaaacggt cgatacggct gttcgagctg cgtccatacg gcgcgcccgg
    13681 gaaggggttc cacgggatca ggttgatctt acacggcgta tctttcagca gttccgccag
    13741 ttggtgcgcg tgttcagtgc cgtcgttaac gtggtcaagc atcacatact caatagtgac
    13801 acggccctga ttggcgttgg atttctccag ataacggcgc accgcagcaa ggaacgtttc
    13861 gatattgtac tttttgttga tcggcacaat ttcgtcacga atttcgtcgt tcggcgcgtg
    13921 cagggaaatt gccagtgcaa cgtcgatcat atcgcccagt ttatccagcg ccggaactac
    13981 accggaagta gaaagcgtga cgcgacgttt agacaggcca aaaccgaaat catcaagcat
    14041 gatttccatc gccggaacga cgttgttcag gttgagcagc ggctcgccca tgcccatcat
    14101 cactacgtta gtgattgggc gctgaccggt gacttttgct gcgccgacga ttttcgccgc
    14161 acgccacacc tggccaataa tttccgatac ccgcaggttg cggttaaagc cctgctgggc
    14221 ggtggaacag aatttacact ccagcgcaca ccccacctgc gaagagacgc agagcgtggc
    14281 acggtcgtct tccgggatat acaccgtttc gacgcgctga tcgccaacgg cgatcgccca
    14341 tttaatggtg ccgtcggatg aacgctgttc ttcaaccact tccggtgcgc ggatttccgc
    14401 cacctctttc agtttgccgc gcaacacttt gttgatgtcg gtcatctcat caaagttgtc
    14461 gcagcaatag tgatacatcc acttcatcac ctgatcggcg cggaagggtt tttcacctaa
    14521 atctttaaaa aactcccgca tctgctgacg gttgagatcc agcaggttga tttttccatc
    14581 tttcgtggtg acgttttcag gtgtgactaa ttgttcagac atatgctatt ccggcctcgt
    14641 tattacacgt tatggcccct ggaggattga aaaaagaaac gccccggtga gcggctgctc
    14701 gtccgggggc gctgcattgt acaaattctg gcgcacggat gccacgtttg cacgcggcat
    14761 ttacgaaatt attaacgggt gcgcgggcac acttcgcctt cgccaaagaa ataagcgatt
    14821 tcgcgagcgg cagattcgac ggaatcagaa ccgtgggtac cgttttcggt caggctgtca
    14881 gcgtaatcag cgcgcagagt accagccagt gcgtttgccg gattggtcgc gcccagcaga
    14941 tcgcggtgac gctgaacggc gttttcacct tccagcacgg aaaccacgat cgggccagag
    15001 gtcatgaatt caaccagacc atcaaagaac ggttttccat cgtgttcagc ataaaagcca
    15061 cgtgcctgtt caacggtcag gtgcagcatt ttggtgccga caattttgaa ccctgcagct
    15121 tcaaagcgcg caaagatatt accaatgacg ttttttgcta ccgcgttcgg tttgatgatg
    15181 gaaaaagtac gttcaatagc catttttacc tctgtaaatt gttctgttgt tgtctgtacc
    15241 agcgtacgag atggcgcgga ttataatgag caacagggcc gttgactatt gatgaaggta
    15301 acattttttt aaaataaaat gagttttagc aacaatcagg gacaaaacag actattgcat
    15361 gacaaatttc actgtcgcga tttgccccac atcatccatc accagcaatt gataatcacc
    15421 tttatccgtc aaatgcaggg tcacgttgcg cccgcgttca gttaacggtt cgccattcag
    15481 aaaccaccag cgttcacctg cccctccact tgattgcaac ggcaaagtcg cttccgctgc
    15541 gcccggtaaa cgtttaataa tcgcgccatc gcgaacgcct gtcagctgca gagggagctg
    15601 ggcatcgtga ccgtatgacg ggcaaattgt cgaggccggt ggtaagcgca cagcgcgacg
    15661 ttctgatgcg ggcagccagg gttccagcgg tagcggccag acatttatca tttcttgtcg
    15721 cgcttgcggg caatcagcgg caacacgttt gccattttca tccagccaga tggggaaacg
    15781 aatgccatta atgccttcct gctccggcag taatagagtt ggcggctgac tcccgtccag
    15841 cagccaggtt gccaggcggc ggcgacagtt accgtcacct tccggcaaag actgtccgcc
    15901 cggccagcag ataacgccac gagtgactga gttcgggcgc gggtcttccg gcagattcgc
    15961 actgcgcgac agtaagatat tattgacctg attcaacaat ggtacggcac tggcaaagcc
    16021 aaactgacca acaacgggcg tgccgtccgg tctgccagtc cagatcccaa tgacatagcg
    16081 agcgttaacc ccaatcgccc aggcgtcacg atagccatag ctggtgcccg ttttccatgc
    16141 cagtggggcg acgcgcggca aggcactatc cggcaagggt ttcgcttcat cagccataat
    16201 ccggcgaatg atccacgccg cccccgacga cattaaaggc cgttcaagca gcggatcgtc
    16261 aggctgtaag cgcaatttgc ctgccttgcc gtggcgagca aacgcggtat acgctgccgc
    16321 catatcttcc agttttgcac cagcgccgcc gagaatgagt gaaagattcg gcgcagcacc
    16381 gttgggcaaa tataacggca atccaacatt gcgtaacttt gccgcaaacc gtttcggtcc
    16441 ataggcttcc agcacctgca cagcaggtaa gttcagcgag cgcaccagcg cctcgctcat
    16501 gctgatcggg ccatgaaaac cgctatcaaa gttacctggt cgataatcac cggtgcgccg
    16561 ggggacgtct tgcagcagtg atgccgggtg gatcaagcct tcatccagcg ccagaccata
    16621 aacaaacggt ttgagcaccg atcctggcga tcggttcgca ttgaccatat caacatgacc
    16681 aaagcgtgaa tcatcgttga gatcaaccga tcccacccag ccgcgaacac gcatatcggt
    16741 atgatcaacc acgatcatcg ccagtgagct gcgcggtggc aaccgccctt tccagttttg
    16801 cgccagttct tccagacgtc gttgaagacc ggcatccagc gtagtaacga ttttgtcgct
    16861 tttgctttta ccgagcatca tgcgcgaaaa cagcggtgcc agttgcggca tttgtcgggg
    16921 tgccagccag atgggttctt cccttgactc ttttacctgc tcacgggacc acgcaccttg
    16981 cacggccatc cgttcgagca ctttattacg cgcggcttcg gcacgctccg gccaacgatc
    17041 cgggcgaaga cggctgggcg cttgcggcaa aaccgccagc attgccgcct cggaatagct
    17101 taaattcgca ggcgactttc cgagataagc ccaacttgcc gcaccgatcc cctgcaacgt
    17161 accgccaaac ggagcgcggt taagatacaa ggtcagaatt tcacgcttag acagatgcca
    17221 ttccagttgc aacgcgcgcc agagctggcg aattttgccg ccaaatgttt tggggtgagg
    17281 atcaagcaga cgagcaacct gcatagtgag cgtgctgcca ccggaaataa cccgtcccga
    17341 agtgagatct tgccatgctg cgcgcgccac cgagaatgga ttcacccccg gatgcttcca
    17401 gaaccagcga tcttcataat tgatcagcgc ttcaaggtaa cgtggagaaa catcttcgat
    17461 tgttaccgga taacgccaga tgccgtcagc atcggcgaag cgccatagcg gcgtaccatc
    17521 ctgcgccacg accactcgtg cgggattgac ttcatgcagc ggtagaggcc agagtttatc
    17581 tgctccccac gctgcaagaa aaaggagaaa tggcgcagct gccagcgtta tccagcagcc
    17641 gcgtttggtt aacaagcgag gcatttacgg tcggacaatc agcagatctt cagccgcgcc
    17701 ggtcgcccgc cattggggaa catacattga ttccaccatc ggttgcggta cctgatacgt
    17761 tcccggcgtc accgcccgcg ccagatacac cagcgttacc ggttggtatt catcaacggc
    17821 aaccgccgcc acaaagcgat cgtcacggaa ctcaatgtgc ttaatactcg cctgctgcat
    17881 ctggttcagt aagttctgca cttcgccacc actttgctcc aggctggcgc taccgttcgc
    17941 cagattctgg ttttccagtt ccagacccgc aggcagcaga tccacgacca acgcatccgg
    18001 cacgctgtta ctggctttta cctgcaacca caccagcacc agatcgccgc tacgtaacga
    18061 gtccagcgat ttgctcttac catcagtacc aagaatatga cgctcgattt gcagcacatc
    18121 gttcgccggt aaaggtgcgg attgcggata accgctggca tccacacgca gccataacgg
    18181 ctgatcgcca ctgtttctca cctgcaaggt ggcaagttga tcgctattca gattgctgtt
    18241 tagcgttttc tcgcctgtca gcggctcagc tgagaaagag gtttgcgcct gccatttacc
    18301 cggtaaatcc tgaatcgtac gggcagccag gaacaacgcg ttactttcct gcgtcgatag
    18361 ccagcgttca ccaaatgcct gctgcgaaag cgtgttcagc aaagtgttct gctcatcggg
    18421 tagcagttta ttttcttcca gcaaggagag cattaacgcg ttgtcgcgca gtggactacc
    18481 gtaatcaccc agccatatcc gctcatcact atttctcggc gttttcagcg ccagcacaat
    18541 cgcttcttca ccacgcgttg catcacccat ggttttcagc gcaacgccaa gttgcagcag
    18601 cggtaaacca gaagcggcat ctgcacgatg ctcccagatt tcacgcagcg cacccagtgg
    18661 tgctttttgc tgacgggcca gcaccagcgc agcgtaagac tgaacggcga atttactggc
    18721 tttgagatta tccgcgtacg ggatcgacat catgcccgga tcttgtaaat agcgcagcag
    18781 acgctcatta ccccggttaa tggcgtctgt cggcacgctg taaccctgtt cgcctgcgcg
    18841 gaccaggaaa tccatcacgt aagccgtcag ccagtactct tcgtcaccgt ttttatccca
    18901 cagcgcaaag ccgccgttat cacgttgcat ttgcagcaaa cgggaaatgc cgatatcgac
    18961 cgatgcacgg cgtttctcat cactgtcgcc tttgatgccc aacgcctgca gttgggctgc
    19021 gttggtataa agtgacggga acaggccgct ggcggtttgc tcaagacagc cgtacggata
    19081 cgcttttaac tctttgatat aacgtgcaat gttcagcggt ggtttgccgc tcaacaacaa
    19141 ttgcccttcc agcgtaacag gcgagaagtt ttgcaatcca tccgccggaa tcgcccatgt
    19201 ctcaccaggc tgtaacgccg taccgtaatt aaccgtttgt gccgggaacg ccggacggac
    19261 gccgattttc cactgcttat gctgatcggc aacggtttca cccggtaacg ctaacccgct
    19321 aatggtggcc tgaatttctc catcgccata acccggcaat gctcgcaccg ggataaacag
    19381 cgtagtacgc acacctggcg ctaattcaac ggctgcgggt gaatcgctga ccagttcaag
    19441 caaaccactg gcggtcaggg caacgttcag tttttgcggt ttatcggtaa gattagtgat
    19501 atccagcgtc agacgcgagg tatcgccact cgccataaag cgcggcatgt tcagttcagc
    19561 aatcaccggt gcggcaacta tcactttgct ttcgttgcta ccgaaatcat ctgccgtcca
    19621 ggcttgcgcc atgacgcgca attcaccgtt aaaatcgcca atcggcagtg taaccgagcc
    19681 ttcgccctgt tcgttgagcg ttaccggcag cgcctgctgc gcgacaatat tgacgtgatt
    19741 gaccggcggt ttaccaccac gtttcagctc atcaccatcg ccaccgaaac gcagagctgc
    19801 cagacgcccc tgaccttcaa taacctgacc gtaaatatcg taaatgtctg cgccatagcg
    19861 tttctgacca aagaacgcct gccacggatc tggcgtgacg tagtcagtaa tattcagcac
    19921 accactatca acggcagaca ccagcacatt cacctgttta ggcttctcgc catttttagt
    19981 gctggcttta attttcacgg ttaatggttg attgggacgc atttttgctg gtgtttccag
    20041 cgccagatcg aggcgacggt tttcatcgcc aagcggcaga tgcaacacac caaccgcgcg
    20101 ttttggcgtc gcggagcgag atttatcgcc aggacgtacc accagcgtac ttaaatacag
    20161 atcatgatga ttccaggttt tatcgaccgg aatcgtcaga tccagccctt gagcccgaac
    20221 atcaatctct tgccaccaca gcggcccttc actggactcg accatcgcat aacctttacc
    20281 cgccgttggc gcggcgatat gcaacttaat ggtgtcgcca gggcgataac tggctttatc
    20341 cagtttcagc gtgacacggt cgggtcgcac tgcgccgcta ccgtcgctgt tgtcctgcca
    20401 gctatagcca gcccagaaac gaacactact gaccgcttca ttcggcgctt tgacttccag
    20461 acgataagcg ccccactcta ccggaaaact gaccttgccg gtttcgtccg ctttcagatc
    20521 cagagtttgt tcattttcga tcagatcttt ttgatcaaac tgtgactgcc agccttcatc
    20581 ttctgaccag ttccagtagt aatcgcggcg ttcgcgaatc aggcgcacct gcaagcccga
    20641 cacggctttt ttcacgcctt gcgcatcgct ataaacgatg tcaaaagcgg cgttactgcc
    20701 ttcatcaaca atgggctgtt tcaccgtgct gtcagtacga taatcgtaaa ccgatttcga
    20761 ggcgaactgc ggacggatcc ccggcaatgc atcggcaggc cagatagcct gctcagcgcg
    20821 gcgcgtcacc gggcgaccgc ccgattccag caaactaccc tggaaaataa cctgtaatgg
    20881 ggaatgcgtt tccttccact ggctttctgt agaaacttcg ccgcgccctt tatcatccag
    20941 cgtcaactga acttcatcca gcgtgcggga aagattttcg gcagctatat cgccgaattc
    21001 aaaaccaggt aaggctgaca cagcttcacg cagtgggcgc aggaaaagtt gcccttgcaa
    21061 agtattacca ttagcaggtg caccatacag gtagtacccc accacggaga atttcacttc
    21121 atctttcggc gttagcgggg ttttctcacc ggtcagattc agcgccatgc gctctggcat
    21181 aaaatcttcg acgtggaaat cccacatccg atactggtta tcgcccgtgt tagcgcgaat
    21241 atgccacata ccggttgccg cattgctatc gagtggccag gtaaagtggt agaggccatt
    21301 ctccggctga ctaacgacgc tcctgagtac ctgcccatcg ggtttaatca cgtctaactt
    21361 gatgggttga ttgggcaacg ctttaccgtc tgcatcacgc agcaaaccat tgaggattac
    21421 cgtttcactt gggcgataaa gatcgcgtgg gccaaacatg aaaaactgtt tgctatagcc
    21481 tggcgcgcca gcaatgttaa attctgctaa gtccagcgcc ggaagtttta aatcgagtag
    21541 cgttgtctga ccgtctttac gcgccaacag taatgccgcg tttttatcat tttccagctg
    21601 cacatgcccc tgagcgtcac tggttgcctg agtcagagtc tgccctttct catttaataa
    21661 agagacttca attccttgct gggccgcgcc gttttccaga ctttgggtaa agatatccag
    21721 acgattgtga taacggtgag ctgaaacgcc gatatcactt aacgtaaaca gcgtcgcggg
    21781 attactgtaa tcgtaacgtc cagcctgatt catcacagcc agatacacgc ccgcctgctg
    21841 aagcggtttg atatcgccca gcggcagcaa taatttttca cgggtgttac gcgcaggatt
    21901 gagatcaaac cgtccggtgt agaccagatc cgccatctgc agcagtttgt ctgactgcca
    21961 gttcgccagc gaattgcggt attcccattg gctaatgaat gctggcagag attctggctt
    22021 aacgcggaag aagttaacat cgacattatt aacgttgagc gccattaccg gcagcccttc
    22081 aacgactttg ccaggcagca gcgaaccacg gctggcaaaa ccgacgctgg gttggatgtc
    22141 gcgggtagtt atagtttttt cgtaatcttt actgaaggtt gcgttgttga gtgctttgac
    22201 ctccttgcca atagtaacga tcaaatcacg tttcggttcg aggtggcgta aacgtaactc
    22261 tttaagatta tctgacagct cccaggcacc atccactttg ccgctttttt tatcgacgac
    22321 atgaataacg cgtgagaaat cctgatccgg gtcgagaggg atggagaacg tcagcaccag
    22381 cgtggctgca ccatcaagtt ggacttcaga gagatccagc aatgtcagca ccttaccggc
    22441 actctgttgg gccagttttt gtctctccgg cacggagagt tttgctgaac tcgcgttttc
    22501 tgaagaggcc gctttagtaa cttcagaagg cgcatctttt ttcaccgctg ttggcgcgtt
    22561 atcgttgttg tcgcaccctg ccagcgccag cattagcatg caggcggcta cgcgtaactt
    22621 tttcattttt catccctgcc acaatggccc gttagcaacg tcgaataatt attatgcgtg
    22681 agaatttacg ttatgtaatt cagtatcacg cccatacaat ccgaatttta gtgagaattg
    22741 tggctcagac agccaaatca gcctctttcg cttgtcgctt ttcactaaac atccgacaat
    22801 ttagcctaac cccggcaaaa atggagatgc ctatgtccac gacatggttt gtaggagccg
    22861 actggctcgc cgaacatatt gatgacccgg aaattcagat tattgatgcc cgcatggcgt
    22921 cgcctggaca ggaagatcgt aacgttgctc aggagtatct gaatggacat attcccggcg
    22981 cagtgttttt tgatatcgaa gcgctttctg atcacacttc cccgcttccg cacatgctgc
    23041 cgcgcccgga aacgttcgcc gtggcgatgc gtgaattagg cgttaaccag gataagcacc
    23101 tgattgtcta tgacgaaggt aatcttttct cagccccacg agcatggtgg atgctgcgca
    23161 cctttggtgt agagaaagtg tcgattctgg ggggtggact tgcaggctgg cagcgcgatg
    23221 atctgctgtt agaagaaggt gcagtagagc tgccggaagg agagtttaac gccgcgttta
    23281 atcctgaagc cgtggtgaaa gtaaccgatg tattattggc aagccatgaa aatacggcgc
    23341 aaattattga tgcccgcccg gctgcacgtt ttaacgcaga agttgatgaa cctcgcccag
    23401 gtttacgtcg cggacatatt cccggagcac tgaatgttcc gtggacggaa ctggtgcgcg
    23461 aaggcgaact aaaaacgacc gatgaactgg atgcgatatt ttttggtcgc ggcgtcagct
    23521 acgacaaacc aattatcgtc agctgcggct ctggtgtaac ggcagccgtg gttttgttag
    23581 cactcgcgac gctggatgtg ccaaacgtga aactgtacga cggcgcatgg agtgaatggg
    23641 gcgcgcgggc agatttaccg gttgagccag tgaaataagt attttacagg caataaaaaa
    23701 ccgccgaatt tggcggtttt ttattgctgg tctggttcgc ggcctttcca gcaggttgta
    23761 ttaccgtagt aatgcaagcg cgtctcagcg gagacaatac tcgccagtaa ctctcttttt
    23821 gtcaagcaaa agagagttat tattgttctg ttagtgtatt atccactgcg gccctttccg
    23881 ccgtctcgca agcgggcgct ggcttaagga aaggatgttc cgcgaccgtt agagcaagcg
    23941 cgtctcagcg cttgctatcg cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg
    24001 catggaccgc acaatgaaga tacggtgctt ctgtatcgta cttattgttt ctggtgcgct
    24061 gttttcccaa gtaaataata accggagtct ctccggcgac aatttactgg tggttaacaa
    24121 ccttcagagc agcaagtaag cccgaatgcc gccctttagg gcggcatatt ttagattatc
    24181 cggttctgtt taaagtcacg caaaaaacca ccccagcgac gttcatagaa tggcgcaata
    24241 tgttcggtaa taaagtggct aattcctttt tccccttttt tcacctgaca aatatcgatt
    24301 ggttcatcgc caggtaatgt atcggtcgct acacttcccg tcgcctgaat aatttcttcg
    24361 atatcaccat cggcttcaat gccaataagt aaattaggct gtgcctcttc gttctcttta
    24421 attgaacaaa taaaagcacg cttcaccggc ttaatggttt taaataaggt ggtgagtgaa
    24481 tcaatcattt gtgctggcgg ctctgcgact tccgataata tcagcgattc accgccttcc
    24541 aggatttcct ggctgctcag cggatttccc tctccaccaa tcaacaaact gatttcacgc
    24601 ggcataaatt ctttaccggt cggcagtttg gcattaagga agagcgtttc gccaagggtc
    24661 atctcaaaca atgtgcgaac gggcattacg acaaatgcct gttcgtcttc aaccgcctgt
    24721 tgaagcgctt ctaacgaggt gaaaaaagga atgacgctgg tgccgtcttc tttttcccag
    24781 tgctgtaaat caagcgcact atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc
    24841 acccagacgg tggattccag tagagtacgg aaaaagcccg ggcggtgcgc cggttcggtt
    24901 gctgcttttt ccagcaggtc ttcaagttcg ttttttgttt cggacatatg aaccacaatt
    24961 cattcaacgt tcggggcgca aatgcctgat gcgctacgct tatcaggcct acaagggatt
    25021 cgcaatttgt tgaatttgca gaatttgtag gccggataag gcgtttacac cgcatccggc
    25081 atattagttt acgccgttaa cagattagct atcgtgcgca caccaagtcc cgtagcgccc
    25141 gcagaccact gttcaaccgg cgctttacgg taagtcgccg agcagtcgat atgcagccag
    25201 ccttgctgat agttctcaac aaagtgcgac aggaagcccg ccgccgtgct cgcgcctgcc
    25261 ggatacgccg cgcttccggt attgttcagt tcggcaaagt tagacggcag ctggctgcgg
    25321 tggaattccg ccagcggcag acgccagaat ggttcgttct cttgtgaggc actcgccagc
    25381 aaacgaccgg caagcgcatc gtcaaaactg aacagcgcgt gataatcatt acccagcgca
    25441 gttttcgccg ccccggtgag ggtcgccgca tcaatgatca tttccggttt ctgcgcactg
    25501 gcatcaatca gaccatcggc aagcaccaga cgcccttcgg catcagtgtt catcacttca
    25561 acttttttac cgttgcgata ggtgatgata tcgcccagct tgaacgcatt gccgctaatc
    25621 aggttatccg cacagcagag gaacagcttc acgcgcttgt tcagtccgcg cgtaatggca
    25681 aatgccagcg ccccggtaac cgttgccgcg ccgcccatgt ccgacttcat cgagtccata
    25741 aacgcagtct gtttgatgct gtagccgccg gagtcaaaag tgataccttt acctaccagg
    25801 cacgcgtaca ccggtgcttc tttatcgcca gttgggttgt aatccagcgc cagcaatacc
    25861 ggagaacgtt ctgaaccgcg cccgacggtg tgcagcccca tataaccttg ctcacgcaga
    25921 tcttcgcctt tggtgatccg ataagtcaca cgatcgcccg cgacgttgct gatcagatca
    25981 acagcacgct gtgccagttg cgatggtccc aattcttctg ccggtgcgtt gatggtgtca
    26041 cgcacccagt cgatgatcat caggcggtta tccagttcct ggcgctgggc atcgtccaga
    26101 tccggccact ccactttacg cgtgcctttc ggggctttgt aaccttgcca gaatgcccag
    26161 cagcgatccg catcccagcc ttcaccgctt aactgaacat gcttgatgcc cagaccgtca
    26221 atcttgcgcg ccgcacgctg gatcagcccc agatcgtctg ccccgttcaa atgcagggta
    26281 atgccgtcat tattaatgct gtaagttgct ttttctcccc agcgcgcgtc ggcaggttgg
    26341 gtagagaggg taatcttcat cgcttctgtc attttagtta tccttctttg taaaagggcc
    26401 gccagttggc agccccagga tttttccgct aaatgattcg cgttgcagga aggcggcaag
    26461 tgagtgaagc cccaggagca tagataacta tgtgactggg gtgaacgagc gcagccaacg
    26521 catctgcggc gtgaagcatg acgcggaaat tattcggcct cgtccagcca gactaacaaa
    26581 atcgcttcga ggattttctc gttggatgcc tgcgggtcgt cgtcgaaatc ttccagatcg
    26641 caaatccact gatgcatatc ggtgaatcga accgttttcg gatcaagatc gggatacgca
    26701 tcgtacagtg cttcgccaat ttcgcggcta tcggtccatt taagtcccat actaacctct
    26761 gttaatgctc acgcgcatgg ttgatagtgt aacgcgggat ttcgaccact aaatcttcgt
    26821 cggtgacgcg cgcctggcag cttaaacggc tttccggctc cagtccccag gctttgtcca
    26881 gcatgtcgtc ttcctgctct gagctttccg gcagcgagtc aaaaccttca cgaacgatgc
    26941 agtggcaggt ggtgcaagca caggattttt cacaggcgtg ttcaatctcg ataccgttac
    27001 gcagagctgc gtcgagaatg gtttcaccgc tattagcttc cagaacagcg ccatcagggc
    27061 agagatcctg atgaggcaaa ataacaatct ttggcatatt aaacctcgtc cacggaatgg
    27121 cctttcagcg cacgacgaac cgactggtcc atgcggcgag cggcgaaatc ctgggtttgt
    27181 ttgtctacgt ttttaatcgc ttgttcgatg gcgtcaacgt catcgccctg cgccacttca
    27241 ctcaggtgag cggcagcatc gtcaatgacc tgacgttctg cggcgcttaa cagcgcggca
    27301 tcagcagcca gcgcgccgtg cagactttcc agcacacgcg ccgcttctac tttttgttct
    27361 gccagcattc gggcttttac gtcctgctcg gcatagctca ttgagtcttt gatcatcgaa
    27421 gcgatttcgc tatcggtcag accgtaagac ggtttgacct gaatagacgc ctcaacgccg
    27481 gtggatttct ccatcgccgt cacgctcaaa agaccgtcgg catcgacctg gaacgtcacg
    27541 cgaatatgcg caccgccagc cggtagcgcc ggaataccac gcagcgcaaa acgcgccagt
    27601 gagcggcagt cctgcaccag ttcgcgctca ccctgcatta catggataga catcgccgtc
    27661 tgaccatctt taaaggtggt gaaatcctga gcgcgggcca ccggaatagt ggtattacgc
    27721 ggaatcactt tctccaccag gccgcccatc gtttcgaggc ccagcgacag tgggatcaca
    27781 tcaagcaaca gcatttcgct gtctggcttg ttacccacca gaatatccgc ctgaatcgcc
    27841 gcgccaatag cgacgacttt atccgggtcg atggaagtca gcggtggacg accgaaaaat
    27901 tcgcctaccc gttcacgcac cagcggcacg cgagtagaac cgcccaccat caccacttcc
    27961 agcacttcgt cagcttctac acccgcgtct ttaagcgcgc gacgacaagc cagtaaggtt
    28021 cgttttacca gtggcgcgat cagttcattg aattgttcac ggctgatttc gccctgccag
    28081 cccgcaacgt taacggtcac ggagtccgca tcgctcagcg cgattttggc tgcaatggcg
    28141 gcatccagca gttcacgctg aacgcggtta tcgctacgat caggaatgcc cgcctgctcg
    28201 cgaatgtaat ccgccagcag atggtcgaaa tcatcaccac cgagcgcgga atcaccgccg
    28261 gtcgccagca cttcaaacac accgcgactt aagcgcagaa tggaaatatc aaacgtcccg
    28321 ccgccgaggt cataaacggc gatcacgcct tcctgaccgg aatccagccc gtaggcgata
    28381 gccgcagcgg tcggttcgtt aagtaagcgc aggacgtgaa ggcccgccag acgcgccgcg
    28441 tctttggtgc cctgacgctg ggcatcgtca aagtacgccg gaacggtgat aactacacca
    28501 tccagctcgc ctgccagggc ttcagttgcc cgcgccgcca gtgctttgag gatgtccgca
    28561 gaaacgcgca ccgggttcag cagccccgcc gccgtttcaa tcatcggcag gccgttttcg
    28621 ctggcctgga attgataagg cagatgcgga tagcgttgct ggatatcagc cagcgagcgc
    28681 cccatcaggc gtttaacaga actaatggtg ttggcggtgt caagcgctgc gttagtacgc
    28741 gcgtcataac ccaccgaatg cccttgctgt tgatagtgaa caacagatgg cagcaggtga
    28801 cggccttcat ggtcggctaa cgtttcggcc tgaccgctgc gcactgtcgc caccagcgag
    28861 ttggttgtgc ccaggtcaat accagccgcc agacgacgct gatgcggcgc agcactcaaa
    28921 ccaggttcac taatttgtaa taaggccatg tttagcttcc agaaattaaa aatcgagcag
    28981 tttttcttcg agttgttcgg cactgctccg cagtttatcg agaaaacgca gcttacgcac
    29041 ggtatccgcc gccgcgtccc acgcctcgtt gtctaactgt tcaaccatca actgatggcg
    29101 ggtatcaaac atctttttca cacgtttgat aaagctttcc agccgcgctt catctttcgc
    29161 ctgttcgatc tcgtccagct cttcgcgcag ctccaactgt tccatcagga acgcggtgtc
    29221 gcgcacagta tgctgctcgc tggcgagatc aaaaccgtgt aaagaaagca aatattccgc
    29281 gcgcattaac ggatgacgca gcgtttgcca ggcctggtta atggttgcag attgctgtac
    29341 ggcggcgagt tgttccgcct ggcttccgct ggcgaattta tcaggatgat actgacgttg
    29401 tagatcctga aaacgcaggc tcagcgcctg ggtatcgagt tgataacggg caggcaagcc
    29461 aaagagggtg aagtaatcca taacattctc agggctgcga tccgccgcga taacggcgaa
    29521 tcaggtaaaa caaaccccac gcgcaggcga ccacggtggg gttatcggta tgcgcatcaa
    29581 acgtggaagc tttcgccgca accacactca tctttaacgt tcgggttggt gaatttaaac
    29641 ccttcattca ggccttcttt tacgaagtcc agctgcgtac cgtccagaaa ttgcaggctt
    29701 ttgccatcga ccacgacttt cacgcctttg tcttcaaaca cgatgtcttc cggcgtcggt
    29761 tcgtcaacaa attccagtac ataagccata cctgaacatc cggaggttct cacgcccaga
    29821 cgcaggccaa accctttgcc gcggttagcc agaaaggtat ttactcgcgc tgctgcactg
    29881 tcgctcagtg taatcgacat aaccaaacct caactcttat tttgcttcac gtttgctttt
    29941 atagtccgca atggcggctt tgatcgcgtc ttctgccaga atagaacagt gaattttcac
    30001 cggcggcagt tcaagttctt cagcaatatc ggtatttttg atcgcctgcg cttcgtcgag
    30061 agacttccct ttcacccatt cggtgaccag ggagctggaa gcgatagcag aaccgcagcc
    30121 gtaagtttta aaacgcgcgt cttcaatgat accttcatcg ttgactttaa tctgcaactt
    30181 catcacgtcg ccacaggccg gtgcacccac catgccgctg ccgacgttct cgtcgttgtt
    30241 gtcaaaggaa cccacgttac gcggattctc gtaatggtcg ataacttttt cgctgtaagc
    30301 cattataaat tctcctgatt ccgataccga ttaatgatga gcccattcga tgctgttcag
    30361 atccacgccc tgcttgtaca tttcccacag cggagaaagg tcacgcagac gaccgatgga
    30421 tttacgaact aactcgatgg tgtagtcgat ctcttcttca gtagtaaaac gacctaaaga
    30481 gaaacggata gagctatgtg ccagctcgtc gttcagcccc agcgcgcgca gcacgtagga
    30541 cggttcgagg cttgctgacg tacaggcgga acctgaagaa actgcgaggt ctttcagcgc
    30601 cataatcagc gactcacctt caacgtagtt gaagctgacg ttgagaatgt tcggcgcacc
    30661 gtgttccagg tcaccgttca ggtaaacttc ttcgatatct ttgatgccgt tccacagacg
    30721 gttacgcagg ccgcgcagac gttccatctc ggtcgccatc tcttcttttg cgatgcgata
    30781 ggcctcgccc attccgacga tctggtgaac aggcagagtg ccggaacgca taccgcgctc
    30841 gtgaccgccg ccgtgcattt gcgcttcgat gcgtacgcgc ggtttacgac gtacatacag
    30901 cgcaccgata cctttcgggc catagatttt gtgaccggag aaagacatca ggtcaacttt
    30961 caactggctc aggtcgatag gcagtttgcc cacgctctgg gttgcatcaa cgtgatagat
    31021 aatgccacga gcacggcaca tttcgccgat agccgcgata tcctgcacca cgccgatttc
    31081 gttatttacg tgcatgatgg aaacaaggat ggtgtcgtca cgcatcgctg cttcaagttc
    31141 tttcaggtcg ataatgccgt tacgctgcgg tgccaggtag gtgacttcaa aaccttcgcg
    31201 ctccagctga cggcaggtat ccagtaccgc tttgtgttcg gttttgctgg tgatgatgtg
    31261 cttgcctttt ttctgataaa agttggctgc acctttgatc gccaggttgt cagattcggt
    31321 tgcaccagag gtaaagacga tttcacgcgg atcagcgccg accagatcgg caatctgatt
    31381 acgggcgata tctaccgctt cttcagcctg ccagccgaaa cggtgagaac gagaggccgg
    31441 gttaccaaag gttccgtcca tcgtcataaa ctgcatcatt ttctcggcaa cacgcgggtc
    31501 caccggcgtg gttgcggagt agtcgagata aatcggtaat ttcattgctc tataaactcc
    31561 gtacatcact caatgcaagg aaccaggcta ccggctggat gtacgaccgt gtttacggag
    31621 cgcgatacgc cccggcctga ttctaatatc ttgttatctt taacctaact gtttcgggtt
    31681 tcagcaagtt gacaaactta cgaatctccg ggagcttaca taagtaagtg accggagtga
    31741 gtatgtgcag tcaacgcaga tgcaacacga aacaggacgg ttaaattaag cgcgtaactt
    31801 aacgtcgatc gcgtcttgta tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga
    31861 cacatccagc acttcctggt tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc
    31921 ggtgagacgg tcgctcaaat cacgccacag cgcgtgggtc aggcatttat cgccgccctg
    31981 gcagccgcct ttaccctgac aacgggtggc atctacagat tcgtcaacgg cgctaatcac
    32041 ttcgccaacg gcgatgctgc tggcatcttt gcctaacaga taaccaccgc ctggtccacg
    32101 tacgctggaa accagaccat ttttacgcag acgggaaaac agttgttcca gataagaaag
    32161 ggaaattccc tgacgttcgg aaatatcagc caacggtacc gggcccgctt cagagttgag
    32221 cgcaacgtca agcattgcgg tcacggcata gcgcccttta gatgtcagtc tcatgtctta
    32281 cttcacctca aactcgcccc tgcccggggt tttttattgt aaaagtgggg gtattgcata
    32341 gcagggtcaa gtctgacatt cccgagtaaa ttggtcaact atttacttga ctgatttagt
    32401 cgggtattta accttcagtg ccattttttt atcgtggcgt ttgtacctgt tgtccttcca
    32461 gaccatgggc gggttgaggg caattattcg cattagcgct ggcctcttcc cctcacccta
    32521 accctctccc cgaaggggcg aggggaccgt ccgtgcattt atctggctta gccctcaggc
    32581 cttattacct ttattctgct gctcaataga agccagaatc ccgcgcagga tattcaattc
    32641 ctggctttcc gggcgcgcac gggtaaacag acggcgcaat ttattcatca cctgccccgg
    32701 atggttttca cggataaaac cggttgccag cagcgtttgt tccagatgac cataaaaacg
    32761 ctccagatca tcgaccagcg gatacggcgt ctcttcatgc tcgacctgct cgccgttttc
    32821 ctgagttgcc agccaggcca tgcgcacttc ataggcaata acctgagccg ccatcgccag
    32881 gttcagcgag ctgtattccg ggttagccgc aatcgcgaca tgataatggc atttctgcaa
    32941 ctcttcattg gtcaagccga cgcgctcgcg accaaacacc agcgccaccg gggtatttgc
    33001 cgcttcagcg acgcttttca ggccgcattc gcgcgggtcg agcatcggcc atggcagcgt
    33061 gcgggagcgt gcgctggtgc ccaccaccag gctacaaccg gctaacgctt cgtcgagcgt
    33121 atcgacgatg tgagcattac caatcacatc gctggcccct gctgccaggg caatcgcctg
    33181 ggagtcgggt ttcaccagtg gattaaccag ccacagattg gttaatccca ttgttttcat
    33241 ggcacgggca acagaaccca tattgccggt gtgtgacgtc tccaccagca caattcgaat
    33301 attttgcagc attgtctttc ttcatctaaa gattattcac gcatcttatc ataaaacgaa
    33361 gacagatgcc gatctcgctg ctatactctg cgccgttttc ccgttcttta acatccagtg
    33421 agagagaccg atgcatccga tgctgaacat cgccgtgcgc gcagcgcgca aggcgggtaa
    33481 tttaattgcc aaaaactatg aaaccccgga cgctgtagaa gcgagccaga aaggcagtaa
    33541 cgatttcgtg accaacgtag ataaagctgc cgaagcggtg attatcgaca cgattcgtaa
    33601 atcttaccca cagcacacca tcatcaccga agaaagcggt gaacttgaag gtactgatca
    33661 ggatgttcaa tgggttatcg atccactgga tggtactacc aactttatca aacgtctgcc
    33721 gcacttcgcg gtatctatcg ccgttcgtat caaaggccgc accgaagttg ctgtggtata
    33781 cgatcctatg cgtaacgaac tgttcaccgc cactcgcggt cagggcgcac agctgaacgg
    33841 ctaccgtctg cgcggtagca ccgctcgcga tctcgacggt accattctgg cgaccggctt
    33901 cccgttcaaa gcaaaacagt acgccactac ctacatcaac atcgtcggca aactgttcaa
    33961 cgaatgtgca gacttccgtc gtaccggttc tgcggcgctg gatctggctt acgtcgctgc
    34021 gggtcgtgtt gacggtttct ttgaaatcgg tctgcgtccg tgggatttcg cggcaggcga
    34081 gctgctggtt cgtgaagcgg gcggcatcgt cagcgacttc accggtggtc ataactatat
    34141 gctgaccggt aacatcgttg ctggtaaccc acgtgttgtg aaagccatgc tggcgaacat
    34201 gcgtgacgag ttaagcgacg ctctgaagcg ttaatgactc aggcgggtga tgtcactcac
    34261 ccgccctcgc ctttcaggcg ctattctgaa atacttcctc accgctttac tttctttcgt
    34321 cactctccca ccatctttcc ccgattaatg gatgataaag aagtcgcaag gaaaggttat
    34381 ggcactgcca gtgaacaaac gcgttcccaa aattctgttt attctctttg ttgttgcctt
    34441 ctgcgtttat ttagtgccgc gcgttgccat caacttcttc tattatcctg acgataaaat
    34501 ttacggtccc gacccctggt cggcggaatc cgtcgaattt acggctaagg acggtactcg
    34561 tctgcaaggc tggtttattc cttcttcgac gggccctgct gacaacgcca tcgcaaccat
    34621 cattcatgct cacggcaatg ccggaaatat gtccgcccac tggccgctgg tcagttggtt
    34681 acccgagcgt aatttcaacg tttttatgtt tgattatcgc gggtttggta aatcaaaagg
    34741 cacgccgtcc caggccggat tgctggacga tacgcaaagc gccatcaatg tggtgcgcca
    34801 tcgcagtgat gtaaacccac aacggctggt gctgttcggg cagagtattg gcggggcgaa
    34861 tattcttgct gttattggtc agggtgatcg tgaaggcata cgtgcggtga tcctcgactc
    34921 cacatttgcc tcttatgcaa ccatcgccaa ccagatgatc cccggcagtg gctacttact
    34981 tgatgagagt tacagcggcg aaaattatat cgccagcgtc agcccgatcc cgcttttact
    35041 cattcacggt aaagctgatc acgttatccc atggcagcac agcgaaaagt tgtatagcct
    35101 ggcaaaagag ccaaaacggt tgatcctaat cccggatggc gaacacattg atgctttttc
    35161 cgatcgccac ggcgatgttt atcgcgaaca gatggtggac tttatcctta gcgcgttgaa
    35221 tccgcagaac taacccatga tcgctagcac gataatcatt cacaaaacca ccttaagaca
    35281 tgctaatcca ctggtcagaa cagtttaaga tgagaaaaat tctgtgacgc ttgccaacat
    35341 ttctgatgat tagcattccc ttcgccattt ccctgagcaa actttagcta ttcttatcaa
    35401 ttatgcttat gggagatcta cagatgatgc ctacgcttgc tccaccatct gtcctttcgg
    35461 ctccccagcg ccgctgccag atcttgctga cgctctttca gccggggttg accgccacca
    35521 tggcaacctt cagcgagctt aatggtgtgg atgatgatat tgccagtctt gatatcagcg
    35581 aaacaggacg ggagatcctg cgctatcatc aactcacact gacgactggt tatgacggta
    35641 gctaccgggt tgaaggtaca gtgcttaacc aacgtttgtg tttatttcac tggctacgac
    35701 gtggtttccg tctgtgtccg tcatttatta ccagccaatt cacccccgcc ctgaagagtg
    35761 aactgaagcg gcgcggaatt gcgcgtaact tttacgacga taccaatcta caagcgttag
    35821 tgaatctctg ctcccgacgg ctgcaaaaac gctttgaatc gcgcgatatt catttcctgt
    35881 gtctgtatct gcaatattgt ttgctgcagc accacgctgg aattacgcct cagtttaatc
    35941 cgctccaacg tcgctgggcg gaatcctgcc ttgaatttca ggtagcgcag gaaattggac
    36001 gccactggca gcgtcgggcg ctccagcctg taccgcctga tgagccactg tttatggcac
    36061 tacttttttc catgttgcgg gttcccgatc cactgcggga tgcgcatcag cgagacagac
    36121 aattgcgtca gtctatcaaa cgtctggtaa accattttcg tgagctggga aatgttcgtt
    36181 tttatgatga acaggggtta tgtgatcagc tttataccca cctcgcccag gcgttaaatc
    36241 gcagtttgtt tgccatcggt attgataata ccctgccgga agagttcgcc agactgtacc
    36301 cacgcctggt gcgcaccacc cgcgcggcgc tggccggatt tgaaagtgaa tacggcgtcc
    36361 atctttctga tgaggaaagt ggtctggtcg cggtgatttt cggtgcctgg ctaatgcagg
    36421 aaaacgatct gcatgaaaaa cagattattc tactgaccgg gaatgatagc gagcgagaag
    36481 cgcagattga gcagcagtta cgcgaactaa cgttactgcc gctcaacatt aagcatatgt
    36541 cggtaaaggc atttttgcag acaggcgctc cgcgtggcgc ggcactgatt attgcgcctt
    36601 ataccatgcc attaccgctc ttttcaccac cgctgatcta tacggacctg acgctgacaa
    36661 cacatcaaca ggagcagatc cgcaaaatgc tcgaatcagc atgagggaac aactttcggg
    36721 cgcaaaaaca ttgccggcag cgccaccagc gccattaccc agaacacgcc gtggcccaga
    36781 tattgataca ggaaaccggc gaaaacggtc atgatagcga tactgccgcc catcgcgacg
    36841 gcagagtaaa ccgcctgtaa acggatgact tcgctaccct ggcgagcagc aatataacgc
    36901 atggcggcca ggtggcagac cgtgaaggtg ccgcaatgca gaatttgcac cactatcaac
    36961 cacggcaacg ccgtagttgc tcccataatg ccccagcgca ctacgccgca aatcgccgag
    37021 atcaacagca tatcgcgtgc actacaacgg cggaaaagtt tattactcag cgcaaagata
    37081 atgacttccg ccaccacgcc cagcgaccac aaatacccca ccgccgaggc cgagtagcca
    37141 gctgcctgcc agtaaatggc gctaaaaccg taataggccg catgtgcccc ctgcaataaa
    37201 caaacgcagg ccagaaagcg ccagttctgg cgaaccagcg ccaaccacgc agaccaaccg
    37261 gtgctctcct gctggcgact tgccccttgt ggctgaatcg tcggacggat gagaaagccg
    37321 agcagcatgg atgccacgcc caacgtcaac agcgcgagga tcacccgata atcaaacata
    37381 gtgaccagtt tgcccgtcag cgccgagcca atgacaaacg ccaccgagcc ccacagtcgc
    37441 actttgccgt aatcaagcgg gaactgcttt tgccacgtat tcgccagtgc atcggtcaac
    37501 ggtaccagtg gtgagaaaaa gaggttaaag ccaatcatca ccagcatcag ccacgctacg
    37561 tgcgcccccg cccagaaggc gacagcaaag agaagtgtca gcagtgccag cacgcgcaag
    37621 gcggaaatca ggcgggaagg atcgctgacg cggggcgcga tgagcaaact cccgaggaaa
    37681 cgggcaacca gacctgcccc caataacagg ccgatggttt ctggcgttaa accaatccct
    37741 ttaagccaga cgctccagaa aggtagaaaa atgccgtaac taaaaaagta tgtgaaatag
    37801 ccgagcgcca accagcgcgt ggattgcaaa accatgagtc cctcccgtca ggaggcgtta
    37861 gtctggggtt aatcgaatgt tggtgcaagt tgaaaaataa gaatattcga gcctgcttcc
    37921 cgatgtctgt aacgccagtt cacaagcact aaaaaactga gatccgcgac gcatgttgca
    37981 aaaataatgc gtaattatgc cgttacgctt gccaaacgtt cctgcacaat ggcgatgaaa
    38041 tcgcgtaatg ccggcttcat ctcccctttc ttccacgcca tcagtaaagc aatggaaggc
    38101 acatttccgg caatgggacg aaaaacaacc tgtccggtat taaaattatt catataaccg
    38161 ggtatcaaag tgacgcccag ccccatgccc accagattca tggtcaccag aatattcgtt
    38221 gccacctgga cgatatttgg ctggctgttt tcttgcgcaa accacgcttt aacgatcggc
    38281 gcaagcgaac cggaatacac cggatcggta ctgacgaaat tcacgccatc cagttgcgcg
    38341 gcggtgatct ctttttcatg tgctaaaggg tgatcaaccg gtaacacaac cactaatggt
    38401 tcgtcaaaaa gctccagata atcaatctcc gggctataaa caggatggcg cattaagccg
    38461 acgtcgagtt caccacggcg aattttttcc tcctgttgcg tggtgattaa actcaccagc
    38521 tcaatcaagg tgtctggctg tctgagacga aacatcggta atacttttgg cagtaaattc
    38581 acttccgccg atggcacaaa gccaatggtt aattgtctgt cttcctgaac aattttccgc
    38641 gcccgtaatt tggcattttc cgcttgttcg aggattgcca gcgcgtcctg gagaaaacat
    38701 tctcccgccg ccgtcaacgc gactttgcgc ttatccctca ccagtaacgg aacaccgaca
    38761 cagttttcaa gatcgcggat ctggctgctt aacgaaggct gtgaggtatg cagtttttcc
    38821 gccgcacggg taaagttcag tgcctgcgcc actgcgacga aatagcgtaa atgccgtagt
    38881 tccatcacct tccccttgtt atcgaaaaaa cgtctcagct ggtagaaatt aactatttca
    38941 catattagca accaaccagc aacatcctta tggcacaaaa atagaaggtc aatacatctt
    39001 atctttcagg attaaaaaat atgaccacac cctcagattt gaacatttac caactgattg
    39061 atacccaaaa tggtcgggtc actccgcgta tttataccga cccagatatt taccaactgg
    39121 agcttgaacg tattttcggc cgttgctggt tatttctcgc ccacgaaagc cagatcccaa
    39181 aacctggtga tttctttaac acctacatgg gggaagatgc ggttgtcgta gtgcgtcaga
    39241 aagacggcag cattaaggcg tttctcaacc aatgccgcca ccgggccatg cgtgtgagtt
    39301 atgcagattg cggcaacact cgcgccttta cctgtccgta tcacggctgg tcttatggca
    39361 ttaacggcga gttgatcgat gtaccgctgg aacctcgcgc ctacccacaa gggttgtgta
    39421 aatcccactg gggactaaac gaagttcctt gtgtggagag ttataaaggg ctgatttttg
    39481 gcaactggga taccagcgca ccgggcctgc gtgattacct gggtgacatt gcctggtatc
    39541 tggatggcat gctggatcgt cgcgaaggcg gcaccgaaat tgtcggcggc gtacaaaagt
    39601 gggtgatcaa ctgtaactgg aaattcccgg cagagcagtt cgccagtgac cagtatcatg
    39661 ctctgttcag ccatgcttct gccgttcagg tattaggggc gaaagatgat ggcagcgata
    39721 agcgcctcgg tgatggacaa accgcccgcc cggtgtggga aaccgccaaa gatgcgctgc
    39781 aatttggtca ggacggtcac ggtagcggtt tcttctttac tgaaaaaccg gatgctaatg
    39841 tctgggtcga tggcgcagtt tcaagctatt accgcgaaac ctatgccgaa gcagaacaac
    39901 gtttaggtga agttcgcgcc ctgcgcctgg cgggtcataa caatatattc cccacgcttt
    39961 catggctaaa cggcactgcc acactccgcg tctggcatcc gcgcggccct gatcaagttg
    40021 aagtgtgggc gttctgtatt actgacaaag ccgcctccga tgaagttaaa gccgcttttg
    40081 aaaacagcgc cactcgtgct tttggtcctg ctggttttct cgagcaggat gactcggaga
    40141 actggtgtga aatccagaaa ttgcttaaag gccaccgcgc ccgcaacagc aaactgtgtc
    40201 tggaaatggg gcttggtcag gaaaagcgcc gcgacgacgg cattcctggc attactaact
    40261 atatcttttc agaaactgcc gctcgtggaa tgtaccaacg ctgggctgat cttctgagta
    40321 gcgaaagctg gcaggaagtg ctcgataaaa ccgccgctta ccagcaggag gtgatgaaat
    40381 gagtgcgcaa gtttcactag agttacatca ccgcattagc cagtttctct ttcacgaagc
    40441 cagcttactg gacgactgga aatttcgtga ctggctggcg cagctcgacg aagagattcg
    40501 ttacaccatg cgcaccacag ttaacgcgca aacacgcgac cgccgcaaag gcgtccagcc
    40561 accgacaacc tggattttta atgacaccaa agaccagctg gagcggcgaa tcgcccgtct
    40621 ggaaacgggc atggcctggg cagaagagcc gccgtcacgc acccgtcact taatcagcaa
    40681 ctgccagata agcgaaaccg acatcccaaa cgtatttgct gtgcgggtaa attatctgct
    40741 ttatcgggca caaaaagagc gcgatgaaac attctatgtt ggaacgcgtt tcgacaaagt
    40801 tcgccgtctg gaagatgaca actggcgctt gctggaacgg gatatcgtcc tggatcaagc
    40861 ggtaatcact tcccataacc tgagtgtact gttctgatga atcgaattta tgcgtgtccc
    40921 gttgcggatg tgccggaggg tgaggctctc cggatcgata cctcgcccgt catcgccctg
    40981 ttcaacgttg gcggcgagtt ttatgccatt aacgatcgtt gcagccatgg taatgcgtca
    41041 atgtcagaag ggtatctgga agatgacgcc acggtggagt gcccgctaca cgccgccagt
    41101 ttttgcctga aaacggggaa agcgttatgc ctgcccgcca ccgatccgct caccacttat
    41161 ccagtacacg ttgaaggtgg tgacattttc atcgacttac cggaggcgca gccatgagcg
    41221 atctgcataa cgagtccatt tttattaccg gcggcggatc gggattaggg ctggcgctgg
    41281 tcgagcgatt tatcgaagaa ggcgcgcagg ttgccacgct ggaactgtcg gcggcaaaag
    41341 tcgccagtct gcgtcagcga tttggcgaac atattctggc ggtggaaggt aacgtgacct
    41401 gttatgccga ttatcaacgc gcggtcgatc agatcctgac tcgttccggc aagctggatt
    41461 gttttatcgg caatgcaggc atctgggatc acaatgcctc actggttaat actcccgcag
    41521 agacgctcga aaccggcttc cacgagctgt ttaacgtcaa tgttctcggt tacctgctgg
    41581 gcgcaaaagc ctgcgctccg gcgttaatcg ccagtgaagg cagcatgatt ttcacactgt
    41641 caaatgccgc ctggtatcct ggcggcggtg gcccgctgta caccgccagt aaacatgccg
    41701 caaccggact tattcgccaa ctggcttatg aactggcacc gaaagtgcgg gtgaatggcg
    41761 tcggcccgtg tggtatggcc agcgacctgc gcggcccaca ggcgctcggg caaagtgaaa
    41821 cctcgataat gcagtctctg acgccggaga aaattgccgc cattttaccg ctgcaatttt
    41881 tcccgcaacc ggcggatttt acggggccgt atgtgatgtt ggcatcgcgg cgcaataatc
    41941 gcgcattaag cggtgtgatg atcaacgctg atgcgggttt agcgattcgc ggcattcgcc
    42001 acgtagcggc tgggctggat ctttaaggaa gcacgatgaa agaaaaaacc atcattattg
    42061 tcggtggcgg gcaagcggcg gcaatggctg cggcctcgct acgccagcaa gggttcaccg
    42121 gtgagctgca tctgttttcc gatgagcgac atcttcctta tgaacgacct ccactctcga
    42181 aatccatgtt gctggaagat tccccgcagt tacagcaggt gttacccgct aactggtggc
    42241 aggaaaacaa tgttcatctg cattccggtg taaccatcaa aacgctgggt cgcgacacac
    42301 gagagttagt gttaaccaac ggcgaaagct ggcactggga tcagcttttt atagcaaccg
    42361 gcgcggcagc tcgaccgctg ccgttgcttg atgcactggg agaacgctgc tttaccctac
    42421 gccatgccgg tgatgccgcc agactgcgag aagttctgca gcccgaacgg tcagtcgtga
    42481 ttgtcggtgc cggaactatt ggtctggaac tggctgccag cgccacgcag cgcagatgta
    42541 aggtgacagt gattgaactg gcggcaaccg tcatgggccg taatgcacca ccgcccgtgc
    42601 aacgctatct tttacagcgc caccagcagg ctggtgtgcg cattctgctc aataatgcca
    42661 ttgaacatgt ggtcgatggt gaaaaagtag aactgacgct gcaaagtggg gaaacgcttc
    42721 aggctgatgt ggtgatttac ggtattggta tcagcgccaa cgagcaactg gctcgcgagg
    42781 ccaaccttga tactgccaat ggcattgtca ttgatgaggc ttgccgcacc tgcgatcccg
    42841 cgatctttgc cggtggcgat gtggcaatca ctcgtcttga taatggtgca ctacaccgct
    42901 gcgaaagctg ggaaaacgcc aataaccagg cgcaaattgc cgctgccgca atgttagggc
    42961 taccgctacc gctactgccg ccgccgtggt tctggagcga tcagtacagt gataacttac
    43021 agtttattgg cgatatgcgt ggcgatgact ggctttgtcg tggcaacccg gaaactcaga
    43081 aggcgatttg gtttaatctg caaaacggcg tgcttatcgg tgcggtcacg ctgaatcagg
    43141 ggcgtgagat tcgcccaatt cgcaaatgga tccagagcgg caaaacgttt gatgcgaaac
    43201 tgctgataga tgagaacatc gcgcttaaat cactgtaacc aggataatta gcgaatatct
    43261 caatgcctgg ggcgtggcga ggtgcaagag tgtgtattac gtttaaatca cattatcttg
    43321 caaagggatt ggttatgaac acactacgtt attttgattt tggagctgcc cgccccgttt
    43381 tgttattaat tgcccgtatc gccgtggtct taattttcat tatttttggt tttcccaaaa
    43441 tgatgggctt tgacggtacg gtccaatata tggcctcgtt gggcgcgcca atgccgatgc
    43501 tggcagcgat tattgcggta gttatggaag tgcccgccgc gatattaatc gtgcttggct
    43561 ttttcacccg tccgctggcg gtgctgttta ttttctacac gctgggtacg gcggtgattg
    43621 gtcaccatta ctgggatatg accggcgatg cggttgggcc aaatatgatt aatttctgga
    43681 agaatgtcag tatcgctggc gcattcctgc tgctggcaat taccgggccg ggggcaattt
    43741 ctctcgatcg gcgttaggaa agatgccgga tgcggcgtga acgccttatc cggcatttaa
    43801 taaattacag ccactctacc cgcaacgaca tctctgaagt tgttgattcc cctggtgcta
    43861 gcgcaatgag atcaccccct tccgggcgat gatgatcatc cggcgcatgg ctcatcggtt
    43921 ccagacagaa gaaatcaaac gcatatcctt tatcaaacgc agggtcggaa acaaagatga
    43981 aataacacgg tgcaggtggc gtcgtttcca tgatgatggc ataaccttcc tgcggctgct
    44041 cgatgcgggc ctgaccattc catccggcaa aaccattgtt cacccactgg cgcggcaacg
    44101 gcgcgggctg gttaaaatcc agttcctgcg gtagctgctc gcaaaactca cccgccagcc
    44161 actgctcccg ctccagccag taaccgctcg cctgtgcctg aatccgcgtt tgcggcgaca
    44221 acggaaaata aggatgccag ccggtaccaa atggcagcgt ctctgcccct tgattggtga
    44281 cagagagcgt caccgtcagc gtatccgccg ttaaatgaaa cgcctgactt acccgatagt
    44341 gatagacacc gctgcgatgt tcatacacca gacacaaact atcatcgctg tgcgagacac
    44401 attgccactc gcccagccag ccatcgccgt gtagatagtg cgcatcccac tcaacattcg
    44461 gttgcagttg atactcacgc ccctgccaga caaaacgatt gccgctcacc cggtttgcaa
    44521 atggcaccag cggaaaacat gaggcatcgg ttgccacacc gcttttttta ccaggacgta
    44581 agagcggcgt cgtatcgcgc cagaagcctt cgattacgcc gccctggtca gaaacgtcca
    44641 gctttagcga cccgtgggat aaggtataga tggtcatccg ctaactcctt aatccgggaa
    44701 gttaatcaca actttcccgc atttgccgct cgccatcagc gcataggcat cacctgcctg
    44761 ttccagcgag aagcgatggg taatggcgtt acgcggccac agtttccagt ccgtcagatc
    44821 atgggcgcat ttttccatat ggaacagact ggtcacccag gagccgataa tccgccgttg
    44881 atggtgcatc agatcggcgc tgacctcgaa ctccactttt ccggtttctc cgatgtaaac
    44941 cacccgtccc cagtcagcgg tggatcgcag tgccagcaag cgacctgcgg cattaccaga
    45001 acaatcgagc gcaacatccg cgccaccgtg ggtgagttcg gcgataatct gcggcagacc
    45061 ttcggtggtt gctaaatagc cgtgatccat cacccctaac tgttttgcca tcgccagacg
    45121 ttccggcagc atatcaacgc cgatgatccg ttttgcaccg cgacctttcg ccagcatcat
    45181 cgccatcatg ccgactggcc ccagaccgac caccagcacg ttatcactgc cggaaacttc
    45241 gccgcgcaaa attccttcat acgctgtacc aacgccgcaa ctgataaacg caccatcttc
    45301 gtagctcagc gcatccggca ggaggatcag atctttttct tccgccagca gatattcggc
    45361 atgaccgccg tcacgctgcc agccgtaagc cgcttttcct ttgccagtac aagagatagg
    45421 aaagccgcgg cggcagttcg ggcaaaaacc acagccagaa atgtgataca ccagcacgcg
    45481 gtcgccctct ttaaaatggc ggcagccttg ccccatcgcc acaatctgcc cgcacggttc
    45541 atgaccgttg ataaatccct ggtataacgg tttatcgggt gccgccgccg tggcacggtg
    45601 ttgatgatag atatagtgga catcgcttcc gcaaatcccg gaggatttca ttttgatcag
    45661 tacctggtta atccccggcg tcggcaccgc aacttcccgc agatcgacgg ttgaatttcc
    45721 tggtaaataa gctgccagca tcgttttcat aaatccctca ttaacaatac gattaatttt
    45781 catccctgcc gcacccgcgc caggggcgtt aaggtttagc gtttcgcttt actgcttcgc
    45841 tgggtcagca agatattcgc cagcaccgcc accacgatga tgacgccgcg taccacctgc
    45901 tggaaaaagg agttaatacc gagcagcacc agaccgttac cgattagcgt aatcaccagc
    45961 acaccaagca atgtaccgaa cagggaaccg cgaccgccgg aaagtgccgt accgccgacc
    46021 acgaccgcgg cgatgacgtc aaactccaga ccgtttgcgg cacctgcgtt accggaaccg
    46081 aggcgtgccg ccagcaaaat gccggtcacc gccgctaata atcccgaaag ggtaaagata
    46141 agaatgcgca ctcgacgaac gttaataccg cacaactgcg ccgccgtggc attaccgcca
    46201 acggcaaaaa ccgagcgccc gaaggcggtt tttcggctaa tgaacacaaa cagcgcaaac
    46261 aacaccatca tgatcagcgc ggatactggc acaccgagaa attgtccgcc cagccagtcc
    46321 agcacctcgt tttcgtcaat cggcactggc agtgcgttgg tcataaacag ccccattccg
    46381 cgcagggcgc tccacagccc cagtgtggca acgaaacttg gtacgttaaa cacgccgcgc
    46441 agcaccccgg cgagcgttcc catcagcgcg cctaacagca acaccagcag acacgccacc
    46501 gccagcggaa cttcaaattg cagcaaaaat gccaggcaca ccgagacaaa agccaccatc
    46561 ggcccaacgc tgacatcaat ttcaccggag ataataatca gcgtcatcgc ccaggcggca
    46621 atcccaatgg tggcggcatc gcgcagcacg ttcatctggt tattcaatga gataaagcct
    46681 ggcgcgttca gggagaagac cagataaaga atggcaatca ccaccagcaa accgatctca
    46741 ttaatatggc gactgacaaa ttgtttgagc gagacgctct tgccctgcgg caatggtaat
    46801 gacgaagcag acatcttcag gttcctcggt aaaattcatc agtgcacaga cagaatggcg
    46861 gacatcagct catccacatt gaccggagcg tgaaactcct gcgagaacgt gccgtgctgt
    46921 agtaacagga tgcggtcaca gaccagcggc aactcctcca cttcactgga gataaacacc
    46981 acgctttttc cttcggcagc cagctcacgg acaatacggt aaatctgctg tttggcttcg
    47041 atatcgacgc cgcgcgttgg ctcgtcgagc aacaaaatct ggctggcggc atagacccaa
    47101 cgaccgatca ccactttttg ctgattgccg ccagaaagcg tgccgatggg cgtttcgcta
    47161 ctggcggcct tgaccgtcat ccgctgcatc acctcttcgg tcaggcggcg gatggtggac
    47221 cattgcagta cgccgttggc gctgattttt tgccgattgg tcagcactgt attttcgtca
    47281 acgcccaacc agggaatgat ccccgcttct ttgcggtttt ctggcgtata gccaatgccg
    47341 cgtttcagca tgtcgccgta atcggggcgc gtgattttct cgccgttgat aacaatttcg
    47401 ccctgttcat actcctccag cccaacaatc gccttcagca attcactgcg ccctgccccc
    47461 agcaggccag caatgccaag cacttcgccg cgacgtaacg taaagctgat atcctccagc
    47521 ttgggcttat ggcgtaatgc acggacttcc agcacggcct gatccacaat ttcctgaggg
    47581 gcaaccggcg caatatcaac gtgatcacgc cctagcatta gcgacacaat atgatgcgtg
    47641 gaggtgtttt cgagcatcac atcgcccgcc acctgaccat cgcgcataac ggtggcacag
    47701 gaggcaatgc ggcgaatttc ttccatccgg tggctgacat aaatcaccgc cacgcccagt
    47761 gccgacatct ttttcaccgc gatgatcacc agttcaactt ccgcactcgc aagcgaactg
    47821 gtaggttcat caagaatgac cacgcgcggc tcgcccttca tcacccgcgc aatttccacc
    47881 agttgctttt gcgccgggct tagcgttgaa acaagttgtt cagggctaac gtcaacgccc
    47941 agcgcctgta agcaacgttg ggcatcctgc gccatttgca ggtaatcaat catgccgttg
    48001 cggcggggcc actgaccgag gcagaggttt tccgccaccg tcagtccctc caccagactt
    48061 aactcctgat aaaccgcacg aacccccagt tcagcggcac ggcgagtcaa cgtagcttcg
    48121 tcaccttcca gtcgcgtctc gccaatccag atatcaccgc tatccgggcg ttcgctgccg
    48181 gtaagcattc gaatgagagt cgatttgccc gcgccgtttt tacctaacag cgcacgaact
    48241 tcgcctttat tgagcgtgaa gttaacgtta tccagcgcaa cgacgccggg gtaacgctta
    48301 tttcctgcca ccacttttgc taccgggact gcctctgttg ccgtgaacat agggacctct
    48361 gcgaatcagc gattagggca gaccatcaac gtgcgttgcc agccactgtt taccatcttc
    48421 cgttttggta tagagatcga ttggcacctg aatcactttt tcaccgtcgg cttgtttatt
    48481 gataaccttc aatgtttgcg cgaaaacagc attgcccatt ttcttaccgg aaatatccac
    48541 taccgctttc agcacctgat tgttttccag ctcctgagca atttcggttg tcatatccga
    48601 accgaaaaca gcaatttttc cggcctgatt ttgattacgt accgctttta ccgcgccgag
    48661 tgtcgcaccg cccgactccc ccataatggc gttgagatcc ggcgtggaga taatcagttt
    48721 ttcaccaacg gaaatcgctt tatctaaaac agtcccttcc tgattagcga caatttgcgc
    48781 gccgggaacg cgggatttta atacttcttc aaatccttta cgtcgctgca cacaaacttc
    48841 aaaggcttcg caattgatga cagcaatttt cggctggtca attttattgg caataaaata
    48901 atcggcggca gcgttaccca gttttttacc aaattccagc ggatcgccga ccagatacgc
    48961 cgagacatat ttatcgcccc ccttttgatt aatacaggtg ttgtagcaaa tcaccggaat
    49021 acccgcttca ctggcgcgac gaacggtacg gctactgcca ttttcagaca ctgccgataa
    49081 aataatggca tcgacattac gcgccacgag ggtatcaaca aaggtacttt ctttcgaaat
    49141 atcaccctgg gcgttagttt caattaactg tacctgtact gaagaatctt ttgccgcatc
    49201 ctgaacgccc tgacgcactc cagcgtaata tccctgggta tcaaggtata ttgcaccaat
    49261 ggtcatttct ttatccgcag ccctggcaaa tagtgcgcta cctaatagcg tagccattaa
    49321 taatagatta cgggttgttc tcattttttt aggcatagtg cctcctgtag ggtttttatt
    49381 aacaacggct tattctaatt attttgtgat gagcggcagc gcgtgccgct caaatattac
    49441 agttaatagg gtttagtgaa cgtagttaaa aataaaaggc atcacggtgg tgaataatgc
    49501 cgctttcggc caggccggat taatcgccgt caattcagcg cgagcagatt catattgcgc
    49561 aacctccccg agtcccgcgg acgccagcat cgccaccatc aggcattttt ctttatgctg
    49621 ctgttgtaaa tcgccataca gcgacaacag gtcaggttgt gaaacagcaa agaaatccgc
    49681 ctcaatactg gttttcgcca tctcttgcgc ccactgtttc atttcactaa acagttgctg
    49741 tgcggtttgc tgttcaccca gcagtcgcag cgccattcct tgccagaaga gataatcaac
    49801 cggctgatcg ttgtaataac tgtggatgtt aatggtgcga tcgccggtcg ccgccagacg
    49861 taaacaacgc atcgcttcag tttcatcgcc ctgcgcgttg gcgcatatcg cctgccagaa
    49921 ccagatgtcg ttatcagttt gccccggtaa acggccttcg cttaaattct ccggataatg
    49981 cagcgcggca tgaagcagtt cgctggcctg ctgcggctct ctggcatcaa gatgctgcca
    50041 ggcgcgtaat aactggttga ggataaactg actggtgacc ttcccttccc cgccttccca
    50101 cgggtggaat ttacgcgtgg cgagaatgtc cgccgctttg tctgcctgac ccgtgagatg
    50161 ccacaaattg agcagttctg cggtcatgtc gtcgcgtttc agcgcaattt cctgattatt
    50221 ttccagacgc gccagtcgtt tctccggtgt ggctccactt aacttatcaa gcaaatcccg
    50281 ttcgaaaagc agacgtgcat cctgcggcgc aagctgataa gcattatcaa gataacgcgc
    50341 ggccagctca taatcgtgtt gcttattcca cgcatggatc gctaacccgc gccagccgtc
    50401 ggcaaactcc ggcgacatct ctacgcaacg ttgccataag gcaatggctt tgttgtagct
    50461 acgtttgttg tagtagaagc aggccagtaa atggcgagca aaccagcact cttcaatact
    50521 ctccagcgcc gccacttctt ccagcgtatt cgggaaacgg acaaactgcg ggaagacatc
    50581 aatggctttt gcgaccagtt cgccacgttc ggctttcggc agcaagctgg cttgcaggta
    50641 aagcggcagc gtgcgctggc agtccagtgc gttcagcatc tctgccgcca gggtgggcat
    50701 tccccagtta atcagttgcc cggcggtcat cagggcgtta acgtcgcgtc cctgacacag
    50761 accgcgccac tgcgcgagag ctgattcgct acgaccatcg aaccagttca gccaccacag
    50821 agtggcgttc agcggataat cgcgcagcag tttctcgcgc tgcacacgcg cgttgtcctg
    50881 acgaccactt aacaccagca gcagattatg caggcaaagc acttcctgat tggttgggca
    50941 ggcgcgaaga ctttgttggc aaaaatccag accagcgtcg aagttaccat tacgcgccgc
    51001 cagtcgtgcc aggccataat agccaccggc tttgctgttg ccgctccaga ccgcacgcca
    51061 gaaatcctct tcggcttgtt gatattgtcc ctgacgttcg taagcactgg cgcgaatcaa
    51121 actcgcctgt ccgcactgcg gatttttgtt cagcgcatgt gcgcgtttca gagcctgagt
    51181 ggcatacgcc accgcttgcg ggaaatcggc gcggttatat tccagcatcg ccagcgccag
    51241 gttacagcgg taatccagcg gatccagcgc cacgccgcgc aggtagtaat cgaacggtga
    51301 acggctggcg tgatgatatt gctccagatg ctgaccgata aaccaggctt catctgtact
    51361 ggtaatgtct tgtgctgcca gtggcgcttt ggcgacgtcc ggcaacggca actcttgcgg
    51421 ctgatgttcc tgataactca gtacaatatt gccgtcggca tcggagagct caatggtcag
    51481 cctttcagga ttgataccgt gcaacacgcc ctggatggcg gtcgcaggca tcagtgccac
    51541 ggcatcatca agtaacgcgt tgcatttgcc gatttcgcgg atcgccaggc ggtatccgtt
    51601 caacggagag atggcataca gcccccactc aatcccccgc ttactacgct ggagttttat
    51661 caccgcatcg cgggaggcat tttgcaccat gcccaaagaa tgataaggca ggaaatactg
    51721 ctcaaaacgc ttctcttcgt aagcatcaag ccaggtaaaa tcgggctggt tatcggcaaa
    51781 aataccggtc atcagttcga tatacggacc gttattgtcg gtcagactct tatcccacgc
    51841 ctggccaaat tcactgtgtc cccaactcca ctgtttttta cctggcgcaa tatggtggtt
    51901 ggcaacgtgc agcaaaccgc catcttcatc gtgacaccac gcgccgacaa aatcgtactg
    51961 cgatttttcg gccatatatg aggttggaac aggcacattt ttatagcgag aaatgtccac
    52021 tccggcggag tagtccactt tgtagtaagt gccggtggcg atggggaaag cggagacggc
    52081 ccgtttgccg tgatcaaaca ccgcagtcac atccggtggg aaaacgctct gatgcccttc
    52141 cccccctttc actgccgggt tggcccacca caagaaatga cgcggcgtgg cgttaccgtt
    52201 atagacgcgg ctggcgattt ccagcgccgc ccggtcaggg cgcagggtga aacctgtcat
    52261 cacctgtaaa ccatgcatcg gctccgtttc gcctacccac accgtctgtg caccgtcttc
    52321 atgggcttcg agggtgaaat caacgggcat aaaggtggtc gggcgatgat gttgcggcca
    52381 gttaaactca atcccaccgg aaatccacgg ccccagcagc cccaccagcg caggtttaat
    52441 gacttcattg tgataaacaa aatcgcgctg tttcacttta tcccacgcgc gatgcacccg
    52501 accgcccagt tccggcagga tcatcacttt gatgtagtcg ttttccagcc acaccgcctg
    52561 ccaggatttc agggttttct gctcgctcag cgtatcggtc acgccgtagg gataaaccgc
    52621 gccggacgat ccctgataaa cgcgattttc gaggaacatg ggatgtatat cctgcggccc
    52681 ggtttcatag gtcgggatct caacgcgctc ttgccacact tttactggag tcatggtgcc
    52741 ctcaatatta acaagacata ctgaattaaa agatttgtgg cagtgtattg aacaatctgg
    52801 caatgttttc gcggaataat cacgcaatta actaaacaag gtttagtgaa gatgagagcc
    52861 tgcattaaca atcaacagat tcgccaccat aacaaatgcg tgattctgga actgctgtac
    52921 cggcaaaagc gcgccaataa atcaacgctg gcccggctgg cgcaaatttc gattccggca
    52981 gtcagtaata ttttgcagga actggaaagc gaaaaacggg tggtgaatat tgacgatgaa
    53041 agccagacgc gcgggcatag tagcggtaca tggctgattg cgccggaagg tgactggacg
    53101 ctgtgcctga acgtgacgcc caccagtatt gagtgtcagg ttgctaatgc ttgtttaagt
    53161 ccgaaaggtg aatttgagta tttacagatt gatgcaccga cgccgcaggc gctgctgtcc
    53221 gaaatcgaaa aatgctggca tcgccaccgt aaattgtggc cggaccgcac catcaatctg
    53281 gcgctggcaa tccacggtca ggttgatcct gtgaccggcg tgtcgcaaac catgccgcaa
    53341 gcgccgtgga caacgccggt ggaggtgaaa tatctgctgg aagagaagct cggcattcgg
    53401 gtgatggtcg ataatgactg cgtgatgctg gcgctggcgg agaaatggca aaataattcg
    53461 caggaacggg atttctgcgt gatcaacgtt gattacggca ttggctcgtc gttcgtgatt
    53521 aacgagcaaa tttatcgcgg cagtttgtat ggtagcggac agattggtca caccatcgtt
    53581 aatccggatg gcgtcgtctg cgactgtgga cgttatggct gcctggaaac cgtcgcctcg
    53641 ttaagcgcat taaaaaaaca ggcgcgggta tggctaaaat cacaaccggt tagtactcaa
    53701 cttgatcctg aaaaactgac tacagcgcag ttaatcgctg cctggcaaag tggagaaccg
    53761 tggatcacca gctgggttga ccgcagtgcc aatgccattg gtttgagtct gtataacttc
    53821 ctcaacatcc tcaatattaa tcagatttgg ttgtacggtc gcagttgtgc ctttggtgag
    53881 aactggctta atactattat tcgccagaca ggatttaacc cgttcgaccg cgacgaagga
    53941 ccgagcgtga aagcgacgca aattggcttc gggcaattaa gccgcgcaca acaggtgctg
    54001 ggaattggct atttgtatgt tgaggcgcag ttacggcaga tttgatggcg caataacgta
    54061 gaaaggcttc ccgaaggaag ccttttttca acagattaac cgtttcgctt atgcgtaaac
    54121 cggataacgt gcgcagatgt cgagaacttt acctttgatg cgctcgataa cagcttcatc
    54181 attgatgctg tccagcacgt cacacatcca gccagccagt tctttcgctt ctgcttcttt
    54241 gaagccgcga cgcgtaattg ccggagtacc cacgcgaata ccggaggtca caaacgggct
    54301 cttcggatcg ttcggtacgc tgtttttgtt gacggtgatg ttagcacggc ccagagcggc
    54361 gtctgcttct ttaccggtca ggtttttatc aaccagatca accaggaaca ggtggttatc
    54421 agtgccgccg gaaaccactt tgtagccgcg ctcgaggaac acttctacca tcgctttagc
    54481 gtttttcgcg acctgctgct ggtaagtttt gaactcaggc tccatcgctt ctttcagagc
    54541 aaccgcttta ccggcgatta cgtgcatcaa cggaccgccc tgaccaccag ggaaaacggc
    54601 agagttcagt tttttgtaca gctcttcgct accacctttc gccaggatca ggccgccgcg
    54661 cggacccgcc agggttttgt gagtggtggt agtaacaacg tgagcatgag gaaccgggtt
    54721 cgggtagacg ccagcagcaa ccaggcccgc aacgtgcgcc atatcaacga acaggtaagc
    54781 accgatgctg tcagcgattt cacgcatttt cgcccagtcc accacgccgg aatatgcaga
    54841 gaaaccaccg ataatcattt tcggcttgtg ttctttggct tgtttttcca gatcggcgta
    54901 gtcgatatga ccggtagcat cgataccgta aggaacgatg ttgtacagtt taccggagaa
    54961 gttaaccgga gaaccgtgag tcaggtgacc gccatgcgcc aggttcatac ccagaacggt
    55021 atcacctggt tccagcagcg cggtgtagac cgcaaagtta gcctgggagc cggagtgcgg
    55081 ctggacgtta gcgtagtcag cgccgaacag ttctttcgca cgatcgatcg ccagttgttc
    55141 aacgatatca acatactcgc aaccgccgta gtagcgtttg cccggataac cttcagcata
    55201 tttgttggtc agctgagaac cctgcgcctg cattacgcgc gggctggtgt agttttcgga
    55261 ggcgatcagt tcgatgtgct cttcctgacg tactttttcc tgctccatag cctgccacag
    55321 ttcggcatca taatcggcaa tgttcatttc acgctttaac atccgcatct cctgactcag
    55381 ctaacaataa aatttttggc ctttataggc ggtcctgttg gacaacggcg aacagtataa
    55441 ccgaatcatt gtgcgataac aggtcttgac aaaggaattt acgcaaacga ttaccttcag
    55501 gctacgcaag gctttggaga ataaagagct tgcaaccgga aacggatttc ttttcaggtt
    55561 tgtgatgcaa atttttcact tcatcacatt ctttctgaaa aacaccaaag aaccatttac
    55621 attgcagggc tattttttat aagatgcatt tgagatacat caattaagat gcaaaaaaag
    55681 gaagaccata tgcttgacgc tcaaaccatc gctacagtaa aagccaccat ccctttactg
    55741 gtggaaacgg ggccaaagtt aaccgcccat ttctacgacc gtatgtttac tcataaccca
    55801 gaactcaaag aaatttttaa catgagtaac cagcgtaatg gcgatcaacg tgaagccctg
    55861 tttaacgcta ttgccgccta cgccagtaat attgaaaacc tgcctgcgct gctgccagcg
    55921 gtagaaaaaa tcgcgcagaa gcacaccagc ttccagatca aaccggaaca gtacaacatc
    55981 gtcggtgaac acctgttggc aacgctggac gaaatgttca gcccggggca ggaagtgctg
    56041 gacgcgtggg gtaaagccta tggtgtactg gctaatgtat ttatcaatcg cgaggcggaa
    56101 atctataacg aaaacgccag caaagccggt ggttgggaag gtactcgcga tttccgcatt
    56161 gtggctaaaa caccgcgcag cgcgcttatc accagcttcg aactggagcc ggtcgacggt
    56221 ggcgcagtgg cagaataccg tccggggcaa tatctcggcg tctggctgaa gccggaaggt
    56281 ttcccgcatc aggaaattcg tcagtactct ttgactcgca aaccggatgg caaaggctat
    56341 cgtattgcgg tgaaacgcga agagggtggg caggtatcca actggttgca caatcacgcc
    56401 aatgttggcg atgtcgtgaa actggtcgct ccggcaggtg atttctttat ggctgtcgca
    56461 gatgacacac cagtgacgtt aatctctgcc ggtgttggtc aaacgccaat gctggcaatg
    56521 ctcgacacgc tggcaaaagc aggccacaca gcacaagtga actggttcca tgcggcagaa
    56581 aatggcgatg ttcacgcctt tgctgatgaa gttaaggaac tggggcagtc actgccgcgc
    56641 tttaccgcgc acacctggta tcgtcagccg agcgaagccg atcgcgctaa aggtcagttt
    56701 gatagcgaag gtctgatgga tttgagcaaa ctggaaggtg cgttcagcga tccgacaatg
    56761 cagttctatc tctgcggccc ggttggcttc atgcagttta ccgcgaaaca gttagtggat
    56821 ctgggcgtga agcaggaaaa cattcattac gaatgctttg gcccgcataa ggtgctgtaa
    56881 tttgatgttg ccggatggaa acatccggca acccttgacg cggtttaaat tgccgcgtcg
    56941 tcctcttcac cggtacggat gcgaatgacc cgtgccacgt cgaagacgaa gattttaccg
    57001 tcaccgattt tgccggtttg cgccgtgcga ataatggtat caacacaggt atcgacaatg
    57061 tcgtccggta cgacaatctc aattttcact ttcggcagaa aatccaccat atactccgcg
    57121 ccgcggtaca gctcggtatg gcctttctgg cggccaaaac ctttcacctc agtgaccgtc
    57181 atgccggtaa tgccgacttc agccagtgct tcgcgaacat cgtccagctt gaagggttta
    57241 ataatcgcat caatcttttt catgctattc cttgaaaagg tcgcctgtct tttgatctgc
    57301 taaacgtaac acataacgcc aattcattcc ttgaaatcgt ttgcatccag ctcgtgtcgg
    57361 gaaagcagtt tataaaattc tgtccggttg cgccccgcca ttctcgccgc gtgtgtgacg
    57421 ttgcctttgg tgatttgcag cagcttacgc aaatagttga gttcaaactg attacgtgcc
    57481 tcaacaaagg ttggcagcgc cgtattttca ccctccagcg cctgctctac taatgcatcg
    57541 ctaataaccg gagatgaggt cagcgccacg cactgttcaa tcacgttgac caactggcgc
    57601 acattacccg gccagctagc ggtcatcagg cgtttcatcg catcagtgga gaacgcgcgg
    57661 acaaacggtt tatgtcgctc tgccgcctgg cgcaacaggt gatttgccag aagcggaatg
    57721 tcttctgtgc gctctgccag agccggaatt ttcaggctga caacgttgag gcggtaatag
    57781 aggtcttcac ggaattcccc gcgcgccatc gcttttggca ggtcgcggtg agtggcggaa
    57841 ataatccgca cgtcgatatc aatatcgcgg ttactgccca gcgggcgcac tttacgctcc
    57901 tgcaacacgc gcagcagttt gacctgcaac ggtgcgggca tatcgccaat ctcatcgaga
    57961 aatagcgtac cgccttccgc cgcctggaat aaaccttcgc gattgctgac agcgccagta
    58021 aacgcgccac gcgcatgacc aaacagctcc gactccagca attgctcggg caatgcgcca
    58081 cagttaatag caataaatgg tttgctattg cgcgggctgg cgttgtggat agcctgggcg
    58141 aaaatctctt tcccggtgcc gctctggccg ttaatcaaaa cgctaacgtc tgattgcgcc
    58201 accagccgcg cctgttccag caaacgcagc atcagcgggc tgcgggtgac aatcgcctcg
    58261 cgccagcgtt catcggtggc tggcgcggat tgttccagcg catcgtcaat tgcctgatat
    58321 agcgcatctt tatcgacagg cttggtgagg aaactgaaca cgccctgctg tgttgcagca
    58381 acggcatcgg gaatagaacc atgcgcggta agaataatta ctggcattcc cggctgcact
    58441 ttctgaattt cagcaaacag ctgcataccg tccatttcat ccatccgcag gtcgctgatg
    58501 actaaatcta ctttttcgcg attcagtacc cgtaatcctt cagcgccact ttccgccgtg
    58561 accacgctgt agccttcgct ggtcaggcgc aggccaagca gtttcagcaa tcccggatcg
    58621 tcatcgacca ataataaatg cgcaggttta tggctcatca ggagtgacct catgggtgga
    58681 tggcgcgggc ttttcaccct cgtgcggcgt atccggcgag aaatttccag ccggtttgcg
    58741 ggtcgagagc tggcgttcaa tatcagtcag gttttccagc ttgcgggtgg tgagttccag
    58801 ttgctgctgt agaacgtgat gttgctggcg caatgtatcc agctcgctgt cgctggcctg
    58861 ctggagcttg ccgtagcgtt ggcgctcttc cgccagttgc agttgcagcg cctgaccgtc
    58921 gcgccagagt tgatacagag ggcgaacctg tgccgggatc tcggtactta gcgcatcaat
    58981 gcgcgcgacc agctggcggc gctcatacgg cgtaattttg gcgtcggcga gcaaaatccc
    59041 ttgtttaaag gtattttgcc agctgccgtc gtcatattgg cgagcttgct gacgtgactg
    59101 cgcaggcatt aaacgatcag cacaatccat cgcccgcagc cagtaaagcg gattggtttc
    59161 ggttgattta ccttgcagcg cccagatgtc gctacattca gtagaaagat agtcagccag
    59221 ttgataaacc ggaatttttt cttctgctgg cgtatcaatg gctggcttat tgtgactctg
    59281 cacgcaaccc agcaatgcca gacatggcag ccctgccagc cacagccgtc ggggcaataa
    59341 tcgttgaaaa atgtgtcgca tattcaccag acttaatgcc tatcccagtg ggcgtaatta
    59401 ttgcaggcag tttggacatg gacagcgcgg agaaaccgga gcgtacacgt cgtacgtgag
    59461 gatttcgagc actgcccggg gccgaactgg caaataaaat agcctgatgg gataggctgt
    59521 tagcattatt tcgtgttttt cgacgacggc aattcaatgc ggaaacaaac gtcttgcccg
    59581 ctctcgtcga ccagatacag ttccccttgc atacggcaaa tacaatccct ggcaatgctt
    59641 aatcccagac cgctgccctt caccgcccct tttcgctggt ggcttccttg aaaaaagggt
    59701 tcgaagatca tggcgcgttc ctcttgcgga atgggtgtgc ctgtattgat gacatcaata
    59761 taaacccgtg caccatgtaa gctgctgcga aggcaaatgt taccggattc agccccgtag
    59821 tgcaccgcat tggagtaaag attatccagt acgctcatca gcagcattgg ctccgccagg
    59881 caagctgttg ctttgagatc gacgtcggta tgcatcattt tagcccgtgc gggcaggcta
    59941 tgagcagaaa ccactgtctc caccagcggt gctaattcaa cattctccag ttccaccgca
    60001 ctgtccgcct gtttacggtt gtaatcaagc agttgttcga tcagtttttg caaattgcgg
    60061 ctgctgctat caagaatgct caccacctct ttttgctctg gcgtaagcgg cccgacaacc
    60121 tggtcagcca gtaattcagt gccctcgcgc atactcgcca gtggcgtttt taattcatga
    60181 gataaatgtc ttaaaaattg atggcgttgg gattccagcc atgacaggcg ctcacttaac
    60241 caaagaatac gttgcccaac cgagcgtaac tcgctcggtc cactgaacga aacgctattg
    60301 cccagagaac gcccttcccc cagacggttg atcatgcgct cgatattttt caccggcccg
    60361 ataatcatcc gcgtgaaaag tagcaccatc accagactca ccagaaatag caccagcgat
    60421 tgccaaccaa aatattgccc acgttcggcg atttcacgct gaagttgctg cccacgagag
    60481 aacaccactg tgcgcgtggc ctgcaccatt tcggtattgg cactggcaaa ggcttcaaga
    60541 cgcgcggcgg cggcagcatc gggaccactg ttgttacact gaagttgggc cagattgttc
    60601 aaatcctgac gcaatgcctg gtaaagttta tcgtctggca gtacgcctgc gtgggcatcg
    60661 agcatttcgc tgtaacgctt gcgctggctt tgataaacct tcgcaagcgt agggtcgtcc
    60721 agcacgcaat actgacggta gctacgctcc atctccagcg ccgcgttggt catcgcttca
    60781 ctacgccggg catcaataag cgtagtgcgg ttaaccagcg ccgcctgatc gctaagcgca
    60841 ttcaggcttt gccaggcttg ccatgccagc accaacaggg gtagtagaat tagcaaaaat
    60901 gccagcatta ccagttgtcg taatgagcgg ggaaaaacgg gccagcgttt caaggtgtta
    60961 ctctcgtcag acgcgaatag cctgatgcta accgagggga agttcagata caacaaagcc
    61021 gggaattacc cggctttgtt atggaataag gcggtgccta actcgacgtt tcgcccgatg
    61081 gttgatatag ctacgctgat atcagaagtt ggacggcagg caccttgttg tgcgtcattc
    61141 gaattttatg tagcacatcc cgaaggggct gacataagtc ggtgaatgag ccactgctta
    61201 ctattatgca gtaactgtgc caataaagaa aatagtttgg taacgtactg attatacgtt
    61261 gctttgaggg atttatggct tcttcgctat ttgaatgtaa atcagccact gtgtcgctaa
    61321 aaagagacaa cttaagataa acttattaga ttacaataaa atcaatcatt taagtgtcgc
    61381 cagaaagcga cacggtaaac cacccattgt cgtgatttac agacacaaaa aagctcccgg
    61441 agttgggagc ttatgatagt ggttggtgct taatgccgga tgcggcgtaa atgccgcatc
    61501 cgggctgcaa aaccaatgaa ctgatgactt accccaactg cttacgcgca ttgcggaaaa
    61561 tgcgcatcca tgggccatcc tcgccccagt tttccggatg ccaggagttg ctgacagtac
    61621 ggaaaacacg ttccgggtgc ggcatcataa tggtgactcg accactttca gtcgtaaccg
    61681 acgtaatacc gttcggagaa ccgttcgggt tagccgggta ggtttcagtg actttgccga
    61741 agttatcgac atagcgcagt gccaccagcc ctttgctttc cagtgccgcc agatgcgccg
    61801 catcacgcac ttccacgcgc ccttcaccgt gagagacagc aatcggcatt tgcgagccca
    61861 ccatcccctg caacagcagc gacgggcttt gggtcacttc aaccaggctg aaacgcgctt
    61921 caaagcgatc ggaggtattg cgcacaaaac gtggccacaa ctcactacct gggatcagtt
    61981 cacgcagatt agacatcatc tggcaaccgt tacatacccc cagcgccagc gtttgcggac
    62041 ggtggaagaa ggttgcaaac tcatcgcgta cacggtcatt gaacaggatt gacttcgccc
    62101 aaccttcacc ggcacccagc acatcaccgt aggagaaacc accgcacgcg accagggcgt
    62161 ggaaatcttc caggcccgtg cgtccagcca gcaggtcact catatgcacg tcgatagcat
    62221 caaagcctgc acggtggaaa gctgccgcca tttcaacatg cgagttcacg ccctgctcac
    62281 gcagtacggc aactttcggg cgcgcgccag tggcaataaa cggtgctgcc acatcttcgt
    62341 tgatatcgaa cgacagtttt acattcaggc ccggatcggc gtcgtttgat ttcgcctggt
    62401 gctcctggtc agcacactcc gggttgtcac gcaggcgctg catctgccag gtagtttctg
    62461 cccaccagac acgcaacgta gtgcggcttt cgctgaatac agtctgcccg ttggcggtaa
    62521 tcacaaaacg gtcaccggaa accgcctgcc caacataatg gacgcaatca gcaagcccat
    62581 gctgtgccag tacggactcg accgcttcac ggtcagcggc acgaacctga atcaccgcac
    62641 ccagttcttc gttaaacaac gccgccaggc gatcgtcacc cagagagccg atatccgcat
    62701 caatgccaca atgaccagca aaggccattt ccgccagtgt gaccaacaga ccgccatcag
    62761 agcggtcgtg atacgccagc agcttacgct gtgcaaccag cgcctgaatc gcgtcataga
    62821 agcctttcag ttgcgcgaca tcgcgtacat ctgccggttt gtcgccaagc tgacgataaa
    62881 cctgcgccag cgccgttgcg cccagcgcgt tattgccttt gcccaaatca atcagcagca
    62941 gtgcgttatc ttcggtagaa agctgcggcg tgatggtgtg acgtacatct tccacgcggg
    63001 caaatgcaga aatcaccagc gacagcggcg acgtcatttc gcgctcttcg ttaccttcct
    63061 gccagcgggt tttcatcgac atagagtctt tacccaccgg gatcgtcagg cccagcgccg
    63121 gacaaagctc ttcgcccacg gctttaacgg cttcatacag gcccgcatct tcaccagggt
    63181 ggcctgccgc cgccatccag ttggcggaaa gtttaatgcg tttgatatcg ccaatctgtg
    63241 ttgcggcaat attggttaac gcttcaccga ccgccagacg ggcagaggcg gcgaaatcca
    63301 gcagcgcaac cggcgcacgc tcgccaatcg ccatcgcttc accgtagtag ctgtcgaggc
    63361 tggcggtagt gaccgcgcag ttagcgaccg gcacctgcca cggacccacc atctgatcgc
    63421 gcgctaccat gccagttacg ctgcggtcac cgatggtcac caggaaagtt ttctccgcca
    63481 cggtcggcag atgcagtacg cgtttcaccg cgtctgcaat ggtgatccct tcacggacca
    63541 gcgcatcgcc cttcgctttc agcgtttgta catcgcgggt catcttcggc gttttaccaa
    63601 gcaggacgtc cagcggcaga tcgatcggct gattatcaaa atgacgatcg tgcagagaaa
    63661 gatgcagttc ttcggtcgct tcaccaatca ccgcgtaggg tgcgcgctca cgcttacaca
    63721 gttcgtcaaa cagcggtaat tgatcggcag caaccgccag cacgtagcgt tcctgggatt
    63781 cgttacacca gatttccagc gggctcatgc ccggttcgtc gcttagaata tcgcgcagtt
    63841 caaatttacc gccgcgtccg ccgtcgctca ccagctccgg catcgcgtta gaaagaccgc
    63901 ccgcaccgac gtcgtggata aacagaattg ggttagcatc accaagctgc cagcaacggt
    63961 cgatcacttc ctggcaacga cgctccatct ccgggttgtc gcgctgtacg gaagcaaagt
    64021 cgaggtcggc atcagactga cctgacgcca tggaagacgc cgctccgccg ccaagaccga
    64081 tgttcattgc cgggccgccg agaacgacca gcttcgcacc gacgttgatc tcgccttttt
    64141 gtacgtgatc ggcgcgaatg ttgccgatcc cgcccgccag catgatcggt ttgtgataac
    64201 cgcgcagctc ttcgccgttg tggctgttca ctttttcttc ataagtacgg aagtagccat
    64261 tcagtgccgg acgaccaaat tcgttgttaa acgccgcgcc gcccagcggg ccttcggtca
    64321 tgatgtccag cgcggtgaca atgcgctcag gcttaccgaa atcttcttcc cacggctgtt
    64381 cgaagccagg aattcgcagg ttggaaacgg agaaaccaac caaacccgct ttcggcttcg
    64441 cgccgaggcc ggtggcacct tcatcgcgga tttcaccgcc ggaaccggtc gccgcgcccg
    64501 gccacggaga aatcgccgtc gggtggttgt gagtttcgac tttcatcaga atatgcgccg
    64561 gttcctgatg gaaatcgtag cggcccgttt cgtggtcagc aaagtagcgg cccacttcag
    64621 aaccttccat tacggcggcg ttatctttat aagcagagag aacgtaatct ggcgtggttt
    64681 cgaaggtgtt tttgatcatc ttaaacagcg atttcggctg ttgctcaccg tcgataaccc
    64741 aatcggcgtt aaaaatcttg tggcggcagt gctcggagtt cgcctgggcg aacatataca
    64801 gctcgatgtc gttcgggtta cgaccaagct ttgtgaaagc atcctgcaga tagtcaattt
    64861 catcttccgc cagtgccagg ccaagacgca ggttagcgtc gattagcgcc tgacggccct
    64921 gccccagcaa atcaacgctg gtgaccggag tcggttgatg gtgtgcaaac agctgctctg
    64981 catcatctaa agcaaaaaag accgtttcca tcatgcggtc gtgcagttca gcggtaacct
    65041 gctgccattg ttcattggtc agcgtaccgg cttctatata gtaagcaacg ccgcgctcaa
    65101 ggcggtttac ctgttgtagc ccgcagttat gggcaatatc ggtcgctttc gaagaccagg
    65161 gagagatggt gccaggacgc ggggtcacca gcaggagttt gccttgcggg gcgtggctgg
    65221 cgagtgccgg gccatatttc agcaggcgtt caagttgtgc gtgctcatca tcgtttaacg
    65281 gcgcattgag gtcagcaaaa tggacatact cggcgtaaat attgtgaacc gggagcctgg
    65341 cagcctgaaa acgtgccagc agtttgttga ttcggaatgc cgacagtgca ggcgaaccac
    65401 gcagaatttc catcataagt ctctcgtctt ctaagctttc ggtgtaccca agggggggaa
    65461 acgggcgtca ttataaagaa tctgatgcgc tgacgaaacc gtttgcgtgg aaataaaatc
    65521 accatcgtga attagcaacg cgtgccgcca atggctgtaa taagttgcca tctggcgcag
    65581 gtttacgcaa aatgccgctc atttaagagt aaacctttca ctattattac gttttttca
//
LOCUS       DAZGJW010000008        64661 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZGJW010000008 DAZGJW010000000
VERSION     DAZGJW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.008"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(132..920)
                     /locus_tag="ACHKIK_000535"
     CDS             complement(132..920)
                     /locus_tag="ACHKIK_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081773.1"
                     /translation="MAAQNTKTIQYRLRNGQSVEVTINNDGVPGEKVSISDLAIEKTI
                     MCHLGFTEEVSKKHGVAIWSAMDTGMRKFITARTPGMTMMDLMQIAPLFECEPLDVFS
                     NPAICQQLYGEVKLAVTPIVLHEGSLAGVWKVERISSYMPFHVNGVITGENQPVSVIK
                     SDLKRAILEASCRVIGLGKQSYVSFPAGPEGPAEILIMDADLLWQIQFLIGKSIIRAE
                     ELDQYITCTMTDEVKSVAIANARNLCRATLAEPQENIPEETESD"
     gene            complement(1123..1611)
                     /locus_tag="ACHKIK_000536"
     CDS             complement(1123..1611)
                     /locus_tag="ACHKIK_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO9081774.1"
                     /translation="MAQRGVNKVILIGTLGQDPDIRYIPNGGAVGRLSIATNESWRDK
                     QTGQQKEQTEWHKVVLFGKIAEIASEYLRKGSQVYIEGKLKTRKWTDDAGVERYTTEI
                     IVSQGGTMQMIGARRDDSQSSNGWGQSNQPQNHQQYSGGSKPQSSANNEPPMDFEDDI
                     PF"
     gene            1781..2338
                     /locus_tag="ACHKIK_000537"
     CDS             1781..2338
                     /locus_tag="ACHKIK_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9081775.1"
                     /translation="MKGKTAAGGGAICAIAVMITIVMGNGNVRTNQAGLELIGNAEGC
                     RRDPYMCPAGVWTDGIGNTHGVTPGVRKTDQQIAADWEKNILIAERCINQHFRGKDMP
                     DNAFSAMTSAAFNMGCNSLRTYYSKARGMRVETSIHKWAQKGEWVNMCNHLPDFVNSN
                     GVPLRGLKIRREKERQLCLTGLINE"
     gene            complement(2630..3649)
                     /locus_tag="ACHKIK_000538"
     CDS             complement(2630..3649)
                     /locus_tag="ACHKIK_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head processing protein"
                     /protein_id="HFO9081776.1"
                     /translation="MTDVLKTVTDRFCLYSNARKGRQNGRQYVLSAVKTMLESKETQE
                     GLRLGELFGYYGHGRRQLTGKLEVPETSVIMVEGRPVVIDNVPACRTVAISVDDNGIV
                     THTQEILNTEPGKIVAAMIESRAGGWSWATGGRESGKIAVTTSFHGVDYVTTPNYISL
                     DHPASAGMFESADSKSLLAESLAAHGYSDESVQAVISHYSKMAELEMMVEATERTAEL
                     ETALLESQGRHLEAMAKIADAEARIALLEKTAGIRDDVLAAMQDELDNLPIFVSAAQK
                     DAFRLKEPGDAKIVATLFESLIKVGARNLPVTKKIKEVPQAANVQAPRETSIITFNNS
                     INPFK"
     gene            complement(3642..5351)
                     /locus_tag="ACHKIK_000539"
     CDS             complement(3642..5351)
                     /locus_tag="ACHKIK_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9081777.1"
                     /translation="MADNKITLSSVRKALAGVFKDNGERDNILLSALAVHGGSGYLFS
                     RAGAPVQLSGFLGGKPGDSGMAGDGLVDGSRFIFDEVQLPEDRLQRYPLLEEMAVYST
                     IATALNIHITHALSFDKKTGQTFSIVPVHNGNDSDYDAAQGLCDELMNDIGRTINKEV
                     AGWAFIMSVFGVAYVRPYAKEGIGITSFECSYYTLPGFIKEFEVSGNLAGFSGDYLKD
                     ASGKMVFADPWAIIPMKIPYWRPKSNLMPVHTGHKAYSLLDNPEERTPIETQNYGTSL
                     LEYAYEPYMNLRSAIRSLKATRFNASKIDRIIGLAMNSLDPVKAADYSRTITQTLKRA
                     ADLMEKRARGANNMPTVTNTLLPIMGDGKGQMTIDTQTIQADINGIEDILTYMRQLAA
                     ALGLDYTLLGWADQMSGGLGEGGFLRTAIQAAMRASWIQQGVEEFIQRAIDIHLAFKY
                     GKVYPEGDRPYKIEFHSVNTALQQEHNDNRDSQANYATIVTQILDAVSNNSVLANSDA
                     FKRYLFSDVLEIDEKISEALVNELKAKSEDDDHLMDSIIKTPPQELAQILESVFKEGN
                     DND"
     gene            5664..5912
                     /locus_tag="ACHKIK_000540"
     CDS             5664..5912
                     /locus_tag="ACHKIK_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="modulator protein"
                     /protein_id="HFO9081778.1"
                     /translation="MKKRYYTVKHGTLRALQEFADKHNVEVRREGGSKALRMYRPDGK
                     WRTVVDFKTNSVPQGVRDRAFEEWEQIIIDNALLLNAD"
     gene            complement(5949..7076)
                     /locus_tag="ACHKIK_000541"
     CDS             complement(5949..7076)
                     /locus_tag="ACHKIK_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HFO9081779.1"
                     /translation="MGNEVYQSQKCKLKYSKSQIDRAAQLIRHGCSDEERQQAIEMIQ
                     NFRELHLYPLMLMKNHLARAAAKVDKDKKILVARRLKRLSTIIDKLERPSLDGGRTSN
                     AIKLTRMQDVGGCRAIVRNLDQLIQLKERLLKSKSVHRIVKEYDYLTPKDSGYSGIHL
                     AYSCFDQKEEQYPWRKTKIEIQLRTQLQHAWATSLEIIDTLEGIKLKTSSEGHPEWRR
                     FFYLAGCLVAHDEKACTLDELVVSQYEGELKELERTLSVRRKLSTYTLALNLTSDANL
                     IKKLPKNHKGHYLVTMRNAENKPNTKNKKMFLVSVRAFKTKEADEALEALNKDDSDPN
                     VLISVLVATDNIKSLKKAYPNYFGSTNQFTKFLDKHLRNEG"
     gene            complement(7179..7820)
                     /locus_tag="ACHKIK_000542"
     CDS             complement(7179..7820)
                     /locus_tag="ACHKIK_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturation control protein"
                     /protein_id="HFO9081780.1"
                     /translation="MDKKICVVSMSVGKPASMTAAWINNELIMAERTSYPERRRDMEL
                     QLLRELREKEEKGFIVLVEEENSFITGRVGQRVRLRDPFMNGRPVLIEAMQIYKELER
                     QKAIKLPRKESGKYILHQSIFDSEHDKKGDEFFNINWSEITTEHVLTLLCCFATEYNN
                     VASGDYIRAMAGEVEARQEPSLLSPLINIILGTQILEQKKVPKGVLTGKHNYF"
     gene            8414..8635
                     /locus_tag="ACHKIK_000543"
     CDS             8414..8635
                     /locus_tag="ACHKIK_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO9081781.1"
                     /translation="MVNANPCARPEFIWRFYSCQKRHYHFVIAPTEDEARSQLPDAPC
                     IFSARFSTDSRNSLSYWCLPVNASAQEGL"
     gene            8632..9744
                     /locus_tag="ACHKIK_000544"
     CDS             8632..9744
                     /locus_tag="ACHKIK_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO9081782.1"
                     /translation="MRTSLVTREEMIEAIEQHTICISTRDIPGVIANYFMITKQLYRR
                     KDKNAVHRILLSDIREYLLEQGHLNYATVAAEARKEAHRMKATNVKSEKIHATSVQES
                     ELVVVQNQSDEIPVLEWLGVRVVTTETLAKGYGTDEANIRKNLSRNPDRFEEGKHYYL
                     LTGSKLKEFKRLVTISHLVSKYTSQVILWAERGAARMSKIVDTNEAWAFFEKLEDSYF
                     RQKEQQPVAIPQTLPEALRLAAELAEQKQLLEQKAHQLNQQLVAAAPKVDFADRVSVA
                     KGILIGNFAKVVGLKQNALFAWLRENGILIASGGRKNVPFQQYINAGYFTVKEVVLDD
                     EDGYQIRLTPQLTGKGQQWLTRKLLDAGLLKPVAAE"
     gene            complement(9984..10295)
                     /locus_tag="ACHKIK_000545"
     CDS             complement(9984..10295)
                     /locus_tag="ACHKIK_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysogeny establishment protein"
                     /protein_id="HFO9081783.1"
                     /translation="MRKDFNIDGKYVVLSVSTNIQSPAVIVTVKLSDRMPDIDSISVA
                     FPVKSMRGAEHFVINASEEEARRGFAKVMSEFGEFLWHVDKALSISSARSKALTASMM
                     K"
     gene            complement(10346..11377)
                     /locus_tag="ACHKIK_000546"
     CDS             complement(10346..11377)
                     /locus_tag="ACHKIK_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO9081784.1"
                     /translation="MTNSLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSDHTWKM
                     LLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRS
                     GLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRN
                     LAFLGIAYNTLLRIAEISRIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVT
                     KLVERWISVSGVADDPNNYLFCRVRKNGVAAPSSTSQLSTRALEGIFEATHRLIYGAK
                     DDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGA
                     MVRLLEDGD"
     gene            complement(11374..11769)
                     /locus_tag="ACHKIK_000547"
     CDS             complement(11374..11769)
                     /locus_tag="ACHKIK_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081785.1"
                     /translation="MFNGLTDAKVNALLNDAKQKAANPGTNENERAMAVAIWELAEEV
                     KRNRLLYTRVYQQFDGASWIDIEEAEYATCKKEKKIVRILHKAATPITVEIPDHRYFE
                     NDDLPGDVIRIIDEILHEQGLTVKITHNK"
     gene            complement(11789..12010)
                     /locus_tag="ACHKIK_000548"
     CDS             complement(11789..12010)
                     /locus_tag="ACHKIK_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase"
                     /protein_id="HFO9081786.1"
                     /translation="MGYSAAKVSTHLELEKNRGYWRAKWFERDSYQLSLSRGEEKIER
                     TRGRWRFYDENHNQVKAEPILYTLLKTII"
     gene            12615..12824
                     /locus_tag="ACHKIK_000549"
     CDS             12615..12824
                     /locus_tag="ACHKIK_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c1 repressor inactivator"
                     /protein_id="HFO9081787.1"
                     /translation="MAFIQPTIDDVRHCSNALSVDPAETDAARAIAEHYSKISNQEYR
                     ITQDDLDDLTDTIEYLMATNQLDSQ"
     gene            12935..13786
                     /locus_tag="ACHKIK_000550"
     CDS             12935..13786
                     /locus_tag="ACHKIK_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein C1"
                     /protein_id="HFO9081788.1"
                     /translation="MINYVYGEQLYQEFVSFRDLFLKKAVARAQHVDTASDGRPVRPV
                     VVLPFKETDSIQAEIDKWTLMARELEQYPDLNIPKTILYPVPNILRGVRKVTTYQTEA
                     VNSVNMTAGRIIHLIDKDIRIQKSAGINEHSAKYIENLEATKELMKQYPEDEKFRMRV
                     HGFSETMLRVHYISSSPNYNDGKSVSYHVPLCGVFICDETLRDGIIINGEFEKAKFSL
                     YDSIEPIICDRWPQAKIYRLADIENVKKQIAITREEKKVKSAASVTRSRKTKKGQPVN
                     DNPESAQ"
     gene            complement(13819..14931)
                     /locus_tag="ACHKIK_000551"
     CDS             complement(13819..14931)
                     /locus_tag="ACHKIK_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFO9081789.1"
                     /translation="MRTSLVTREEMIEAIEQHTACISTRDIPGVIANYFMITKQLYRR
                     KDKHAVHRILLTDIREYLLEQGHLNYATVAAEARKEAHRMKATNVKSEKIYAPSVQES
                     ELVVVQNQPDEIPVLEWQGVRVVTTETLARGYGVDEANIRNNLSRNLDRFEEGKHYFL
                     LTGLKLREFKNRVTGSYSVGKNARSLTLWTERGAARMSKIVDTNEAWAFFEKLEDSYF
                     RQKEQQPVAIPQTLPEALRLAAELAEQKQLLEQKAHQLNQQLVAAAPKVDFADRVSVA
                     KGILIGNFAKVVGLKQNALFAWLRENGILIASGGRKNVPFQQYINAGYFTVKEVVLDD
                     EDGYQIRLTPQLTGKGQQWLTRKLLDAGLLKPVAAE"
     gene            complement(14928..15149)
                     /locus_tag="ACHKIK_000552"
     CDS             complement(14928..15149)
                     /locus_tag="ACHKIK_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO9081790.1"
                     /translation="MATIPTPAHSEFIWRFYSCQKRHYHFVIAPTEDEARSQLPDAPC
                     IFSARFSTDSRNSLSYWCLPVNASAQEGL"
     gene            complement(15168..15287)
                     /locus_tag="ACHKIK_000553"
     CDS             complement(15168..15287)
                     /locus_tag="ACHKIK_000553"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO9081791.1"
                     /translation="MVVLAGLPKGRPVPFCAGSLNPVSATAIEIETSRGSSLN"
     gene            complement(15509..16993)
                     /locus_tag="ACHKIK_000554"
     CDS             complement(15509..16993)
                     /locus_tag="ACHKIK_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO9081792.1"
                     /translation="MARSCVTDPRWRELVALYRYDWIAAADVLFGKTPTWQQDEIIES
                     TQQDGSWTSVTSGHGTGKSDMTSIIAILFIMFFPGARVILVANKRQQVLDGIFKYIKS
                     NWATAVSRFPWLSKYFILTETSFFEVTGKGVWTILIKSCRSGNEEALAGEHADHLLYI
                     IDEASGVSDKAFSVITGALTGKDNRILLLSQPTRPSGYFYDSHHRLAIRPGNPDGLFT
                     AIILNSEESPLVDAKFIRAKLAEYGGRDNPMYMIKVRGEFPKSQDGFLLGRDEVERAT
                     RRKVKIAKGWGWVACVDVAGGTGRDKSVINIMMVSGQRNKRRVINYRMLEYTDVTETQ
                     LAAKIFAECNPERFPNITIAIDGDGLGKSTADLMYERYGITVQRIRWGKKMHSREDKS
                     LYFDMRAFANIQAAEAVKSGRMRLDKGAATIEEASKIPVGINSAGQWKVMSKEDMKKK
                     LNLHSPDHWDTYCFAMLANYVPQDEVLSVEDEAQVDEALAWLNE"
     gene            complement(16993..18186)
                     /locus_tag="ACHKIK_000555"
     CDS             complement(16993..18186)
                     /locus_tag="ACHKIK_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO9081793.1"
                     /translation="MTWDDHKKNFARLARDGGYTIAQYAAEFNLNPNTARRYLRAFKE
                     DSRTADSRKPNKPVRKPLKSMIIDHSNDQHAGDHIAAGIAEKQRVNAVISAAVENAKR
                     QNKRINDRSDDHDVITRAHRTLRDRLERDTLDDDGERFEFEAGDYLIDNVEARKAARA
                     MLRRSGADVLETTLLEKSLSHLLMLENARDTCIRLVQEMRDQQKDDDEGTPPEYRIAS
                     MLNSCSAQISSLINTIYSIRNNYRKESREAEKHALSMGQAGIVKLAYERKRENNWSVL
                     EAAEFIEAHGGKVPPLMLEQIKADLRAPKTNTDDEERQTAVGGPSLEDLDKVARERAA
                     SRRADAALWIEHRREEIADIVDTGGYGDVDAEGISNEAWLEQDLDENEEEDEEVTRKL
                     YGDDD"
     gene            complement(18272..18724)
                     /locus_tag="ACHKIK_000556"
     CDS             complement(18272..18724)
                     /locus_tag="ACHKIK_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Late promoter-activating protein"
                     /protein_id="HFO9081794.1"
                     /translation="MLLNWQGRHFMEINHSRITSYEIADYMIRTKSFLSAKELAAILE
                     KEYPHLDVDKRDVYLRLKAIAVSKYSSVLIDDSTRPRRFQIHSLNPEFFRRSRAPRRF
                     DEKLQNELYMTQDEKERREHQPWVMARQLFNKVARQHRHYGNATSARI"
     gene            complement(18813..19856)
                     /locus_tag="ACHKIK_000557"
     CDS             complement(18813..19856)
                     /locus_tag="ACHKIK_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9081795.1"
                     /translation="MKAVITPFVQKELGVATFKVDQEVRKLVEAGRKFIMEPVPRELI
                     EHMDDGLVVSEQTMATNEALQPFFNSDELFRRIGGIDALVAWLRRKEGQCQAADRSWC
                     DNHIVHAERDNSAVLLCWHHDNHYRMRGFNELKETLHNNRVNWILDVARQEMGLSNSH
                     DLSIQELCWWAFMRNMMHLMPEEVCRISINKMKATPQDSGPLKEADIRPYDDRATAYV
                     QMMEERAAPMRAKVCPVDVDSDPDMAHFKIPKLQSLKLPEYMDFVASRPCCGCGAAGA
                     GAHITPYIVRHSRLCAHDIYAIPLCQSCQRDIERDRDNWEKTHGRLAMHQRLFFDYAL
                     GVGAITSHSSSVR"
     gene            complement(19884..20063)
                     /locus_tag="ACHKIK_000558"
     CDS             complement(19884..20063)
                     /locus_tag="ACHKIK_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HFO9081796.1"
                     /translation="MARKYNKLSREALKMLLDGVSRREVKQYLVGKQIGARTAIAVLC
                     RQEMVVLKQRMPGSR"
     gene            complement(20068..20448)
                     /locus_tag="ACHKIK_000559"
     CDS             complement(20068..20448)
                     /locus_tag="ACHKIK_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFO9081797.1"
                     /translation="MRHISPEELIALHDANINRYGGLPGMSDPGGAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTV
                     GAATGEISVSSVADTLRRLYGSAE"
     gene            complement(20448..20669)
                     /locus_tag="ACHKIK_000560"
     CDS             complement(20448..20669)
                     /locus_tag="ACHKIK_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO9081798.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            complement(20742..21131)
                     /locus_tag="ACHKIK_000561"
     CDS             complement(20742..21131)
                     /locus_tag="ACHKIK_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO9081799.1"
                     /translation="MGFPSPAADYVESRISLDQQLIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCAMEGEYRIKRYRKYPRQHLEDLSTGKKEALPVDDDGCT
                     GSNAVFGVITHVINDIRSGEFDDCPVI"
     gene            21306..21641
                     /locus_tag="ACHKIK_000562"
     CDS             21306..21641
                     /locus_tag="ACHKIK_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9081800.1"
                     /translation="MPNRIPLDPVLPKNFDCTPNEKRSKAQLDAWWDHPYGVTEHDGK
                     IVVYCLNGGAWDRPSVLGLANNYDEACELAERQQASWVKTRSEPTFMFSKEPPFILAR
                     MPQRPDHQQ"
     gene            21672..21887
                     /locus_tag="ACHKIK_000563"
     CDS             21672..21887
                     /locus_tag="ACHKIK_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081801.1"
                     /translation="MNLFSLKQEERDRVEVSPTLDHNRMNLAQLAWYSKELEMSIARL
                     ENEKAAIQVQHEVVLNRIREMQNDNRG"
     gene            complement(21948..22472)
                     /locus_tag="ACHKIK_000564"
     CDS             complement(21948..22472)
                     /locus_tag="ACHKIK_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFO9081802.1"
                     /translation="MTKFGKLEAHLLTRNYRHDIHPYRQWIDESEGVVTFPLYRFDGM
                     LAGYQTYRPGAPKQHSNPKMARYFTRSHGRQLLWGTYLPLKDGPIWITESIFKSAAVH
                     NAGGNSWALLGSTFSAGLRRQLAMLPYDFRVIGDNDAAGELLVKSFGKGFVAPDLDEL
                     QPHEVSHLIFSHSQ"
     gene            complement(22469..22774)
                     /locus_tag="ACHKIK_000565"
     CDS             complement(22469..22774)
                     /locus_tag="ACHKIK_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081803.1"
                     /translation="MMTGELYFKMARERRVHLDRIFHLQKRVEELERRLNCYPVDMVS
                     AIPPIPIEMQIRLWMEEYGMPWEIFFCFDHKQWVDELDNSFPYFTENTCPVCRKNGI"
     gene            complement(22774..23058)
                     /locus_tag="ACHKIK_000566"
     CDS             complement(22774..23058)
                     /locus_tag="ACHKIK_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin PHD"
                     /protein_id="HFO9081804.1"
                     /translation="MPTKAELQARIDILEKENASLKGMLARAERELSGKLLPEELPPA
                     DIPDRVSWWMKYFRAPWEAFWCYDHRRWCDELDSSFPYFAEGNSCPECRS"
     gene            complement(23042..23731)
                     /locus_tag="ACHKIK_000567"
     CDS             complement(23042..23731)
                     /locus_tag="ACHKIK_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081805.1"
                     /translation="MAQKEMTTLTTAYLQQLVFFAGEATCHPDANYLLEFERLASPGI
                     VLELAQQVLALRQKEQHESNTCRLNFEQWLEQQRGKIDVDCGCVSTETFMHWLRVAYE
                     AGNYPDIPDIPDSSVPAPGKGVTGERIRIKPHVYRELVNRLHDTAIKCAGTQQLRERI
                     SRVLGDVITPDHHKQAEKSDLERCHLEAALNIKPGHTLGIIDALLVHKMARALLPLVA
                     EKHEADHANES"
     gene            complement(23713..24087)
                     /locus_tag="ACHKIK_000568"
     CDS             complement(23713..24087)
                     /locus_tag="ACHKIK_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081806.1"
                     /translation="MMTLKHFLDRPLWAAAAGYDFNYMDCMSYTANAYDHSFSLLFNS
                     LRILPETEVGELHLWILGFIAAGVGIAVWPFIFWLVAVVVWFKCKTYRKKYFLGDGMT
                     DIAKMNIEKWTKECEKKWRKKK"
     gene            complement(24094..24387)
                     /locus_tag="ACHKIK_000569"
     CDS             complement(24094..24387)
                     /locus_tag="ACHKIK_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081807.1"
                     /translation="MWRGNNHGGSQMILTEYTFDHKTNKSRSVYLLRHNSRVRNTVLE
                     QNLTVEMDNLGNFKPTISLDDFPCGLSEREAMLKLAEWLQRLSIAIEDNWPEP"
     gene            complement(24411..24692)
                     /locus_tag="ACHKIK_000570"
     CDS             complement(24411..24692)
                     /locus_tag="ACHKIK_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO9081808.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRLVNCKYDRL
                     IITKGYPKREDFSRRIDVPYNGYEIKTITHPHFGDKPVKVFAIKVKINS"
     gene            complement(24692..24952)
                     /locus_tag="ACHKIK_000571"
     CDS             complement(24692..24952)
                     /locus_tag="ACHKIK_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eaa protein"
                     /protein_id="HFO9081809.1"
                     /translation="MMDVKEKVLQVMRSRAALQDKALGGEYPFRMATWNLRLAMEKEF
                     PDEEWRSADLRKILMELAKDGTVSKDTHASRIGQAVWRLEVR"
     gene            complement(24949..25395)
                     /locus_tag="ACHKIK_000572"
     CDS             complement(24949..25395)
                     /locus_tag="ACHKIK_000572"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO9081810.1"
                     /translation="MRQALAWLEGHSCSDDRLGKADNQPASGNQAAESNRGNEWTGNP
                     DIDNAIIMLDRIDTLENCDDDRIEAVKAVLRRLAGNSPVTPDGWISCSERMPPQDDWI
                     LIYSKHGEYMAGQVQGEYVELSDGTLSWLGNALFWMPLPEPPQEAK"
     gene            complement(25672..25806)
                     /locus_tag="ACHKIK_000573"
                     /pseudo
     CDS             complement(25672..25806)
                     /locus_tag="ACHKIK_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25803..26135)
                     /locus_tag="ACHKIK_000574"
     CDS             complement(25803..26135)
                     /locus_tag="ACHKIK_000574"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081811.1"
                     /translation="MADRKKRFRKNPSLGMGDWRFFISEPGIISIEDLPPGWGLLHVV
                     NGRVRKVHGWPKGNCCWGNPEDKPFIGNKQVECDYMLSALRRMELRGHLNEIYDGVIV
                     NKKEGNAA"
     gene            complement(26140..26318)
                     /locus_tag="ACHKIK_000575"
                     /pseudo
     CDS             complement(26140..26318)
                     /locus_tag="ACHKIK_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26320..26964)
                     /locus_tag="ACHKIK_000576"
     CDS             complement(26320..26964)
                     /locus_tag="ACHKIK_000576"
                     /inference="COORDINATES: protein motif:HMM:NF025303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO9081812.1"
                     /translation="MSKIDYQALREAAEKATKGSYIVGHTSGNQHENITGVFVCQKWK
                     GEPGGVIAECHVNCLVETDAQAYANAEFIAEANPATVLALLDERERNQQYIKSRDQEN
                     EDIALTVGKLRVELEAEEKTSAARLEALDRTHKMFQREQCRAEAAEKRIAELESGSQA
                     QKLVEAIIVAIENEQERLFDEDYLMDSKECIDVIREEVKRWNDSRAAGIRIKGE"
     gene            complement(26961..27662)
                     /locus_tag="ACHKIK_000577"
     CDS             complement(26961..27662)
                     /locus_tag="ACHKIK_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO9081813.1"
                     /translation="MSEINYRALREIAKQATQGEWVAFISPGTGTYAVHTPGDERCED
                     VIKWTGFDGQKNAENNARHVAAFNPKVALELLGEIKCLEDTNIDATCRISELETNLAA
                     LVAENAGLKHAMAVTLEHVSVTDAGQAGVAAMIINDALHHSETPATDAFLAEIRAEAR
                     NEGINYTASRLAAAFNHGFINKSLREVFDVTRMILSAKEELANEPYPIDGLSGEYAEK
                     SLEEWAEQIRKGGSQ"
     gene            complement(27659..27898)
                     /locus_tag="ACHKIK_000578"
     CDS             complement(27659..27898)
                     /locus_tag="ACHKIK_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081814.1"
                     /translation="MFKQNEKAIAQIADYIPRACRGMQLQEAKARLEKKIALYIDDGC
                     DAAVLNAAFAPALNSHTRESFFSCIEAQLRKGGSQ"
     gene            complement(27891..28094)
                     /locus_tag="ACHKIK_000579"
     CDS             complement(27891..28094)
                     /locus_tag="ACHKIK_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081815.1"
                     /translation="MTIVKTHTGTVITKDGPQVKKLHQTERMWVVGKNEFYHKDTGRR
                     HFAENTRRRLLLDTIKPIEVKHV"
     gene            complement(28178..28898)
                     /locus_tag="ACHKIK_000580"
                     /pseudo
     CDS             complement(28178..28898)
                     /locus_tag="ACHKIK_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675631.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29094..29600)
                     /locus_tag="ACHKIK_000581"
     CDS             complement(29094..29600)
                     /locus_tag="ACHKIK_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-phosphatase"
                     /protein_id="HFO9081816.1"
                     /translation="MSIFNKHAHQERPYIVIVDIDGTISEATEDRLHLLPPPGKGALT
                     KDWNEFNLACDTDTPITPVIDMVRQLFNVYTVWFVTGRCEIARDKTRAWLRKYVTNGA
                     EPLLSMRPATDDRNDGPAKIDLLKKIGLSKIAFALEDKIEVARVFRSHGVLTFMVREY
                     ENALLHQQ"
     gene            complement(29673..30935)
                     /locus_tag="ACHKIK_000582"
     CDS             complement(29673..30935)
                     /locus_tag="ACHKIK_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081817.1"
                     /translation="MSTSAQNQSIENVSIPDVLNAGIPAIIQNIRAAQRRVSCDDLTA
                     RFFDNAVQSAEMLHAQLIDVYNAEADSHNSLVDAAENMQLDLGLKGKEIEELQLQIEH
                     LKRQQQDAIDDATHDANQRADNAERISIELETKLNEMTAMVELRNSQISTLKSQYKEI
                     MKLDPFNLEKRYNKAKSERQELRKQVADLNQQLKKTIKDASEARVAFANKKAEVTALV
                     NENAKFATLKKEMYGITERRFPASKLHPTLGQISFFPRLLAYGISSPKEFNNERPYIV
                     SKLDFAYQFCCDMGYAIDIRINEWLMPNFQPLAIFREFQPEGWVEFFHELICKEMESR
                     RPELVRRVEWAQEVMLADAELPFEPEFIDDLATKGLHTLFDVVTRRHEQLVVELGLEE
                     TAARRLLDVCYARSDAWEKENGGTIYVR"
     gene            complement(31237..31938)
                     /locus_tag="ACHKIK_000583"
     CDS             complement(31237..31938)
                     /locus_tag="ACHKIK_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081818.1"
                     /translation="MKIALVFRSGGDYNASDVQWLVNQLPKGYEIICLTDLKRLHVPG
                     VKVVPLINQWQKCRGWWAKIELFRPDITDDLFYLDLDTVIAGDIRPILEDPPTSFTML
                     RDFYHPQYRGSGALWIPNSVKAHIWSAFWQDPEGWIARCVTTECWGDQGFLRKVMGDD
                     TPAFQDLYPGWFVSYKADVVEPGSKYASARYSRGNGALPKDCRIIFFHGKPRPREVSE
                     DWLPLISAFFEQESE"
     gene            complement(31935..32612)
                     /locus_tag="ACHKIK_000584"
     CDS             complement(31935..32612)
                     /locus_tag="ACHKIK_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFO9081819.1"
                     /translation="MIAPTIYHRIDGTKYRNVWVVGDLHGCYTRLMSELHRVDFDPAQ
                     DLLISVGDLIDRGTENVKCLELLQMPWFRAVMGNHERLMIDALSPDGNVNNWLMNGGQ
                     WFFMLDTDQEILARALVELVRRLPYIIELNTGQETIVIAHADYPDNEYQFGKEVPLFN
                     VVWARERISDSMDDIGGEISGADRFIFGHTPVKSPKTFWNQQYIDTGAVFCGNLTLMK
                     VKGDGAA"
     gene            complement(32609..33235)
                     /locus_tag="ACHKIK_000585"
     CDS             complement(32609..33235)
                     /locus_tag="ACHKIK_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081820.1"
                     /translation="MFPKNKFRGSDRVIIVGSGPSAANFVAPRGVPIIAVNGAIDWLN
                     RASYFFTLDPSPDNMRRVGRGRRRRGVCYCMALPDVKEREVRDGVLCFRRVAERGTEP
                     KNTNSPEWWAWRWSAHFGLCEDENEIASGNSAYGALNLAFHIGFKHVALVGVDATQEP
                     RVHSGGTPKNLSHLPLLFQSAREQIDVVSCGKMGGIPQMTLKEWLKNT"
     gene            complement(33737..33892)
                     /locus_tag="ACHKIK_000586"
     CDS             complement(33737..33892)
                     /locus_tag="ACHKIK_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081821.1"
                     /translation="MSSKVNYESLASVMPRNEQETDAVVDPVIAEMNARLEAEFAAEN
                     EHTTQGD"
     gene            complement(33959..34537)
                     /locus_tag="ACHKIK_000587"
     CDS             complement(33959..34537)
                     /locus_tag="ACHKIK_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO9081822.1"
                     /translation="MLRFTEEEFQAFSERRNKGRSRPKTKKDPFLSLTPVKEVSPHAK
                     ALAALAKNPDLRVGNCEHYEQVFIFDYFERNYPEIYELLHATPNGGKRSKATAGKMKA
                     EGQKKGYPDMSLDKACGIYHGMRIELKEPNGKAPTKEQIAWMRRLREEGYYVVLAYGA
                     EQAITAILEYISLKKGEAIEHVLNGDKWLHAA"
     gene            complement(34645..34785)
                     /locus_tag="ACHKIK_000588"
     CDS             complement(34645..34785)
                     /locus_tag="ACHKIK_000588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081823.1"
                     /translation="MAERVDDAELSMNQLEALKDMAIDNIRKQAQVVSQVFTGKCRYC
                     NE"
     gene            34932..35309
                     /locus_tag="ACHKIK_000589"
     CDS             34932..35309
                     /locus_tag="ACHKIK_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081824.1"
                     /translation="MATSITTTQSTRQYPLSRYDDRNIADPILRAELRKEVMLMCESN
                     DKNLTIYYVLPDEQYRPDLLAYRMWGIAELRWVVTLAAGLEDESQGMTVGKKLKLPPA
                     TWIREMIRHFQYDGQVIGTLSIA"
     gene            35319..36536
                     /locus_tag="ACHKIK_000590"
     CDS             35319..36536
                     /locus_tag="ACHKIK_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9081825.1"
                     /translation="MPTEYARDNLGRYQTDGLSAKDFNKVFDLIRKQQRQNRRNARRT
                     LTPRIMGMRNRELEAFLSLGKKKDGTYFTPEDIRSFNTSRQAHKTKFKSTVPGITYAQ
                     LVAQSTSIDIKRANNKVSDGTGIKAATFLGLKHNLALISVNASDESVHQHHRVRIRFE
                     EWDKAVEEIAEDGAKKARIAADLCKGRVSFDCDCGRHQYWYRYMATAGNYAVAPPKEY
                     AFPKIRNPDLTGVACKHVLHAMTRFQSPTWHKAIIIALEKAAEQVAFGDDKRKTTTYF
                     KGELAKSLARNRTTTTDQAKAAREYELYLKSQDALGKKLRAKDSATDNVRRLLKKART
                     TANRKNAELKASRVREAQARAEADALKKALQTQANNLIKFFMSQGMDKAAATAQARSI
                     LETQINEARKRKG"
     gene            36540..37268
                     /locus_tag="ACHKIK_000591"
     CDS             36540..37268
                     /locus_tag="ACHKIK_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9081826.1"
                     /translation="MAGFFDDMFEDTEPSQQVTGDNLPDTESDPDIPGEGSELIEEED
                     IDAEIETDGVNVGNIVDPVEDNHLPNLDHGLLSDSGVRHRYQGHAVFNNLVRMDWLKA
                     IKLDPDSFDAVLYRAIPYRDKNAPETASEIIEPNQRIYDYQDPELITALDCPDEMDAF
                     YALYDGSDNTGISDSALILRLAAVNVPVGSMLEWLEQLSDGTTIRRFWYIHKIFNYGT
                     ARVGSLFYCVPSRAFEGNFIGDSE"
     gene            37255..38040
                     /locus_tag="ACHKIK_000592"
     CDS             37255..38040
                     /locus_tag="ACHKIK_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate"
                     /protein_id="HFO9081827.1"
                     /translation="MILNNQEWLLAIFKKKGLTPTGKLEFATIDGIDSALAQALNEAF
                     DSQVVSFNDRINQSFREFLKRTPRDRITLGTFSDVKEWLSSFEADRAGRKDTASAGPV
                     NKLAMPLVNLSRSPAFSIYEGELCRDNYDEGHVTNENDEIEALVSTIPFSLEYSLWIA
                     SDEKESLGMVTTALAFWLRMYASLGQASFTHIANVGGYEIPVTCYIEGQKSIAFQDLT
                     TGTADNRLFAVGLNLTVVAELPILAYMQQTTGTITVKAKILEE"
     gene            38042..39058
                     /locus_tag="ACHKIK_000593"
     CDS             38042..39058
                     /locus_tag="ACHKIK_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9081828.1"
                     /translation="MATKTTTAPETDSKRTQLFLQSVSIGQNEIPREMIVGCTYVEPG
                     ELSGPQLMLMVRDSTAYVVNKLGVKFGTIMTVSLGDPEGHGGILFSEEFFVLKAPRKD
                     DTVLIYAFSNPVRLLKVPSTSAQYFVDKPPSAVVSSLAPGLKVNADSFRKTSTYHLNV
                     GEKPTKVLQEIARDTGSMCWASRGTINFKSMEKMANAAPSLTYESANPNTSGFTISQF
                     NILNADYEYQRRHNYRMASYDMTKGVVYSGNQEDPIKFTSNPDPTALANYNKFILPRL
                     DMLVEGNAALTPGTTLKIVVHNTAGDGELDESIPDKMIVMSVTHFEDRFRFVSRAQLG
                     VVNG"
     gene            39051..39683
                     /locus_tag="ACHKIK_000594"
     CDS             39051..39683
                     /locus_tag="ACHKIK_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HFO9081829.1"
                     /translation="MGSLTGKYRAVVVSVDDPKGLMRTQIRVVGMMDGLPDASLPWAE
                     AILSNANTFSPFLPGDKVWVEFPYNGDSRWPLIIGYAQDASGGAPNVPPEASGQGEGY
                     VPPEVEGAPAQPSTSAKKDFISSRNGLMEIRTAGGAWAVTHLKSGTTIGFNEAGELYA
                     ISQGPAFISSAGNLDIKSGADVALKAGGSMAIEASGNLSIKAAQVSVDKA"
     gene            complement(39754..40788)
                     /locus_tag="ACHKIK_000595"
     CDS             complement(39754..40788)
                     /locus_tag="ACHKIK_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO9081830.1"
                     /translation="MRTSLVTREEMIEAIEQHTACISTRDIPGVIANYFMITKQLYRR
                     KDKNAVHRILLSDIREYLLEQGHLNYATVAAETRKEAHRMKASKPDVVVTKPLTATTP
                     APVMDAIPNTGDTIDSQTLLKMVNEARKLCGEPLVRNNKFIEKVVDELEGETYTKSVG
                     RKNGANIDIITMTFKQALRVAARESKAVRRSLIDKLEELQQASTASPAIPQTLPEALR
                     LAAELAEQKMQLEQQLVAAAPKVDFADRVSVAKGILIGNFAKVVGLKQNALFAWLREN
                     GILIASGGRKNVPFQQYINAGYFTVKEVVLDDEDGYQIRLTPQLTGKGQQWLTRKLLD
                     AGLLKPVAAE"
     gene            complement(40785..41006)
                     /locus_tag="ACHKIK_000596"
     CDS             complement(40785..41006)
                     /locus_tag="ACHKIK_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO9081831.1"
                     /translation="MVNANPCARQEFIWRFYSCQKRHYHFVIAPTEDEARSQLPDAPC
                     IFSARFSTDSRNSLSYWCLPVNASVQEGL"
     gene            complement(41386..42384)
                     /locus_tag="ACHKIK_000597"
     CDS             complement(41386..42384)
                     /locus_tag="ACHKIK_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081832.1"
                     /translation="MQWNEEKPMNILIIGRKFEAISDVKTYTEMWAYNLACAFSEAGV
                     TLQYHRPYSPGVESPEDYVEAVLTAATACSAKAILAPGLRYFTTVPREIGMQLCRRFS
                     GWVAQVYDGSMLDSAPVDITFTVRDDTWRYLDNPGRLERHNRFNKHVGWAANQELFHL
                     ETKTDDVLRIFVDHAAFDVSGFDHSLSILMNLQRLTVPYEARTLTDDGLVTIDPGNIS
                     VTPYRRTPVPATEFAAELRKSDVFIVTHPESLGLTVLEAAMCGALILTPPDCLPPDRL
                     ALVNHMVIKSRIDWDEVIARVDRVKNAEKVQCHTWSAIAEKMLETFITQKPSRGND"
     gene            complement(42384..43748)
                     /gene="dnaB"
                     /locus_tag="ACHKIK_000598"
     CDS             complement(42384..43748)
                     /gene="dnaB"
                     /locus_tag="ACHKIK_000598"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO9081833.1"
                     /translation="MSASPLESMPNSLSAEQAVLGGLMLDNCRWDEVADRIVTDDFYT
                     SAHREIFSEMERLLSHGKPIDLITLAEALEQNGKLERAGGFAYLAEMSKNTPSAANIC
                     AYADIVRERAVVREMISVANEIAEAGYAQDGRGSNELLDMAERRVFEIAEKRQKSGSG
                     PKDIASILDATVSRIEELFQRPHDGVTGLDTGFTDLNKKTAGLQPSDLIIVAARPSMG
                     KTTFAMNLVENAAVRNDKPVLVFSLEMPSHQLMMRSLASLARVDQTRIRTGQLNDDDW
                     ARVSGAMGILMDKQNIFIDDSSALTPTELRSRARRVYKENGGLSMIMIDYLQLMRVPE
                     LQDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRESGAIEQ
                     DADLIMFLYRDEVYHPDSEMKGIAEVIIGKQRNGPIGTVRLAFNGQYSRFDNYAGADW
                     QEDY"
     gene            complement(44029..44104)
                     /locus_tag="ACHKIK_000599"
     tRNA            complement(44029..44104)
                     /locus_tag="ACHKIK_000599"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44069..44071),aa:Ile,seq:cat)
     gene            complement(44132..44959)
                     /locus_tag="ACHKIK_000600"
     CDS             complement(44132..44959)
                     /locus_tag="ACHKIK_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO9081834.1"
                     /translation="MKKTLISAAIILGSLCLTGCDRVEPGNVGIKVNKLGDDKGIGEV
                     VGVGRYWTGLNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPAKVTTV
                     FQTYRKGVDDITDTDLRQKVADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSNTAVPFVPVK"
     gene            complement(45909..45984)
                     /locus_tag="ACHKIK_000601"
     tRNA            complement(45909..45984)
                     /locus_tag="ACHKIK_000601"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45949..45951),aa:Thr,seq:tgt)
     gene            complement(45989..46075)
                     /locus_tag="ACHKIK_000602"
     tRNA            complement(45989..46075)
                     /locus_tag="ACHKIK_000602"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46039..46041),aa:Tyr,seq:gta)
     gene            complement(46081..46155)
                     /locus_tag="ACHKIK_000603"
     tRNA            complement(46081..46155)
                     /locus_tag="ACHKIK_000603"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46121..46123),aa:Asn,seq:gtt)
     gene            46512..46772
                     /locus_tag="ACHKIK_000604"
     CDS             46512..46772
                     /locus_tag="ACHKIK_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081835.1"
                     /translation="MASGFLGRYHHQIIVCIYFNGQFLNRRKVANHDENPEKNAHPKM
                     GKLEARNALRCCHLGVWSTWQLGVMMGKESLPRHTCRRACRG"
     gene            complement(46831..50118)
                     /locus_tag="ACHKIK_000605"
     CDS             complement(46831..50118)
                     /locus_tag="ACHKIK_000605"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HFO9081836.1"
                     /translation="MKELCYGSVCSGIEAASIAWEPLGMRPAWFAEIEPFPSAVLALR
                     WPHVANLGDMKKLAKKVLAGEIESPDVLVGGTPCFTAGHMVLCKNGYKPIENVCPGDY
                     VVSHLGRLQQVKRVGSKIANTGLLNAVGQPLGIRTTNDHPFLAVRWKAQNTRKNGTYF
                     KRELLSEPEWRAACDMPGYQWCALTNFNIAFPDICSRFLSEEQAMYLAGAYVGDGYIR
                     RWRGKSKKAVVFGINCQKLRKFHCRIPENIFSVASEIRGSIKVTLNDTCYANWLNEHF
                     GELSHAKRIPAWVMSHPLRHVFLQGYLDTDGTPSGKAGFRINSVSSALAWGVAGLSQT
                     CGYVSSVSFIEVEPKKVIEDRVVNQRNYYQVTICPQKLSRKSRLAHGMLLRTVKEFKS
                     VGLDTVYNIEVEGDHSYILNGAVVHNCQAFSIVGLRGGLDDERGALTLKYVELANAID
                     DKRSESFLKPTVIVWENVPGVLSSADNAFGCFLAGLAGEDVPFEPGDRPESGKSNAFW
                     RWDGKTGCHVPKWPQCGCIYGPQRKVAWRILDAQYFGVAQRRRRVFVVASARTDLDPA
                     TVLFEFEGVRRNIAPSRKKKEIASAIIANGAAISGESLNPCLHADMPPSMKSTKAVNA
                     FRMAAFGEYIDDETASTVKARDFKDATDLAVFSSTGAGFWSEGHGTLRAREQESHEHL
                     VTLAFPERMSGTQHAATKNTSPSLMAKNPTAVCYEVRNAEVAVRRLTPVECERLQGFP
                     DGHTLIPTEKRKKVNSDELAYLRNHYPDLSEEEAAMLAADGPRYKAIGNSMAIPVMRW
                     IGDRIAKAACRQKEGSETKERKVKPAAEFERSIFKWAGGKFGVLEQIFRYLPEGKRLI
                     EPFVGGGAVFMNAGYQENLLNDVNADLINFYKTLQREAHSLITLAHRFFQDYNTQEGY
                     LAVRNAFNKQVYDDLHRAAAFLFLNRHCFNGLTRYNQAGEFNVGYGKYKTPYFPLQEM
                     EAFLGAEGRSEFVCGDFAAVIEAAGEGDVIFCDPPYEPLPNTEGFTNYSGHDFKFEEQ
                     KRLVSLLTDAHRRGAKVLITNSGAPNIRELYHDSGFRVEPLFARRSVSCKGDTRGVAH
                     DVIAILL"
     gene            complement(50115..51020)
                     /gene="rdgC"
                     /locus_tag="ACHKIK_000606"
     CDS             complement(50115..51020)
                     /gene="rdgC"
                     /locus_tag="ACHKIK_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO9081837.1"
                     /translation="MFKHWKNITIYKLSREADLTDLEDKKKMILFTPCGSQDMAKFGF
                     VSPFGDNSEVIAMHGNGFILVEAKRETKILPPPVIQRAIQEKIEKLEQEQARKLKKTE
                     KDSLKDEVLHSLLPRAFSKFSVIQAIYDGSTKRIYINASARQAEDMLALMRRSLGSLP
                     AVPLSVENPIELTLTDWVRDGSAPQGFQMGDAAELKAVLEDGGIARVKKQDLGSDEIS
                     THLEAGKLVTKLALDWQNRIKFTLDHNFSLTSVKFADGLLEQNSDIDSEDVAQRLDAD
                     FFLLTSEISCLVDALVNALGGEAKQ"
     gene            complement(51013..51297)
                     /locus_tag="ACHKIK_000607"
     CDS             complement(51013..51297)
                     /locus_tag="ACHKIK_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081838.1"
                     /translation="MQMELISRKEFDSRVTSGELDNLQAIKVKEGFCLIGNQSGINRV
                     FMLRRTDLKPFVWKNEIGPSSYAQTRGCHNLAFFYKDELSVVDIQGLQHV"
     gene            51760..52548
                     /locus_tag="ACHKIK_000608"
     CDS             51760..52548
                     /locus_tag="ACHKIK_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081839.1"
                     /translation="MLEKDYQLSAYKKLAAAGGMKTPGAITSARNSANTAKLLAEELT
                     GLILDTIVYPDTITSYVSTIRTTTTGLTNIGELATKHADLLAGYADLSMLLQLDIGWD
                     VYCRANEREVSELPISIAIGDVNITKSLEDAVNALNTSSLVAAMGEINQALNTGSESS
                     SGSGSGGGTATPPPALTEEQIESLKVATEQFGVVFNQTTAPTTALQQQYERANESANV
                     AITAYNHAIGTALAEASANKASTASAVAALVPDSVLDELNKAAR"
     gene            52588..53010
                     /locus_tag="ACHKIK_000609"
     CDS             52588..53010
                     /locus_tag="ACHKIK_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ppfA"
                     /protein_id="HFO9081840.1"
                     /translation="MSFFSTLKTALSLKEKLAATGVLVLICALVGAGFAWERHQLKQA
                     MEKIGSLDQAVKERDKSIMDLNQTIETMNKAEQHFHSQEVKNESEQAKYADRQMERKA
                     EVQKQLVAAGNVRQRIPADTQRLLRESISEFNADADKG"
     gene            53036..53176
                     /locus_tag="ACHKIK_000610"
     CDS             53036..53176
                     /locus_tag="ACHKIK_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081841.1"
                     /translation="MCRMPEFSSEYFDDLPAYILDTETMLMGINRKNRNVNDYNRAIS
                     GN"
     gene            53188..53580
                     /locus_tag="ACHKIK_000611"
     CDS             53188..53580
                     /locus_tag="ACHKIK_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081842.1"
                     /translation="MSDKVTVKQTINKATSIYKIEHITVGKPGSEQYRHAFELADQLG
                     LKHPDCIEHVFPTYADEQCTHVLTEEDFFSTEEREGVDRCIGVICSSVSYELFPNVHE
                     NGGIGYQFLYEGDELKCYEHGLLIESVE"
     gene            53914..54798
                     /locus_tag="ACHKIK_000612"
     CDS             53914..54798
                     /locus_tag="ACHKIK_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO9081843.1"
                     /translation="MLEENKGFLSVEEVAGNTGEIHSLKPNNNSTIQPIALLRLGVFV
                     PTLKSTNVALRRGSSVTTNTTNATEELSSLKIVEQEGYEGIEIHGPRLDMDTDFKVWV
                     GITSALFDYAPDDDGIITLPFSEFADRCGYPRKRLSKAFRKSIDDSLTRIQQTVVKFR
                     FPAAKGHLNNINVNLLAYSSLNTELDVIEIQPQKQLSELYYVDYKRILKLKMLDKLGR
                     KETAKVLYTFFEALPANPAPVSIERLRARLNLKSSVSVQNSVIRKAMKDLEAIEYLKF
                     SEIKNGRKIGFQIHKRNP"
     gene            55091..55900
                     /locus_tag="ACHKIK_000613"
     CDS             55091..55900
                     /locus_tag="ACHKIK_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFO9081844.1"
                     /translation="MQEEKQHYLYVLVPENGDTFKIGISCGPLARFKGLQVSPDFALS
                     RVYRGTRLAMVNLERALHATFFPWNAPWEKSAGGGHTEWFTRECLDKVLAHIEYLNDM
                     WGGILERIKSNDLLQRPVDAARSFEKELDVTSIVTFKDDAGMRDVAYVSISGYEPDAI
                     RAQCELLKAMFMLRTKYPWETGRVCFPMEELTATIDSQLYHDNPEKFFSLLAGNGLNC
                     VSGLGRSRIQHASLFGPFFYDRHGYFEAELPALTRAIDTIDFERLFAALSK"
     gene            56069..57265
                     /locus_tag="ACHKIK_000614"
     CDS             56069..57265
                     /locus_tag="ACHKIK_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9081845.1"
                     /translation="MSDSSQLHKVAQRANRMLNVLTEQVQLQKDELHANEFYQVYAKA
                     ALAKLPLLTRANVDYAVSEMEEKGYVFDKRPAGSSMKYAMSIQNIIDIYEHRGVPKYR
                     DRYSEAYVIFISNLKGGVSKTVSTVSLAHAMRAHPHLLMEDLRILVIDLDPQSSATMF
                     LSHKHSIGIVNATSAQAMLQNVSREELLEEFIVPSVVPGVDVMPASIDDAFIASDWRE
                     LCNEHLPGQNIHAVLKENVIDKLKSDYDFILVDSGPHLDAFLKNALASANILFTPLPP
                     ATVDFHSSLKYVARLPELVKLISDEGCECQLATNIGFMSKLSNKADHKYCHSLAKEVF
                     GGDMLDVVLPRLDGFERCGESFDTVISANPATYVGSADALKNARIAAEDFAKAVFDRI
                     EFIRSN"
     gene            57282..58283
                     /locus_tag="ACHKIK_000615"
     CDS             57282..58283
                     /locus_tag="ACHKIK_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO9081846.1"
                     /translation="MSKKNRPTIGRTLNPSILSGFDSSSASGDRVEQVFKLSTGRQAT
                     FIEEVIPPNQVESDTFVDQHNNGRDQASLTPKSLKSIRSTIKHQQFYPAIGVRRGTGK
                     IEILDGSRRRASAILENVGLRVLVTDQEISVQEAQNLAKDVQTALQHSIREIGLRLMR
                     MKNDGMSQKDIAAKEGLSQAKVTRALQAASAPEELVALFPVQSELTFSDYKTLCAVGD
                     EMGNKNLEFDQLIQNISPEINDILSIEEMAEDEVKNKILRLITKEASLLTDKGSKDKS
                     VVTELWKFEDKDRFARKRVKGRAFSYEFNRLSKELQEELDRMIGHILRKSLDKKPKP"
     gene            58508..60214
                     /locus_tag="ACHKIK_000616"
     CDS             58508..60214
                     /locus_tag="ACHKIK_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081847.1"
                     /translation="MSNLREYQNRIADIAKRSKAVLGWASTAQFGTDNQFIKDDAARA
                     ASILEAARKDPVFAGISDNATAQIATAWASALADYAAAHKSMPRPEILASCHQTLENC
                     LIESTRNSMDATNKAMLESVAAEMMSVSDGVMRLPLFLAMILPVQLGAATADACTFIP
                     VTRDQSDIYEVFNVAGSSFGSYAAGDVLDMQSVGVYSQLRRRYVLVASSDGTSKTATF
                     KMEDFEGQNVPIRKGRTNIYVNRIKSVVDNGSGSLLHSFTNAAGEQITVTCSLNYNIG
                     QIALSFSKAPDKGTEIAIETEINIEAAPELIPLINHEMKKYTLFPSQFVIAAEHTVQA
                     AYEAQREFGLDLGSLQFRTLKEYLSHEQDMLRLRIMIWRTLATDTFDIALPVNQSFNV
                     WATIIRGKFQTVYRDIIERVKSSGAMGMFAGADAASFFKQLPKDFFQPAEDYIQTPYV
                     HYIGTLFGNVKVYEVPAGICKNLTTENIQFSSMDVLCYVRDENPGKAGFVTGDAVPAI
                     PFQHPTTPALVNRTTLWGSAINDMHPRNGADYFTRVTLTMAKKGGLNFISGDTIDAGD
                     SE"
     gene            60275..61864
                     /locus_tag="ACHKIK_000617"
     CDS             60275..61864
                     /locus_tag="ACHKIK_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081848.1"
                     /translation="MSQYSIQQSLGNASGVAVSPINADATLSTGVALNSSLWAGIGVF
                     ARGKPFTVLAVTESNYKDVLGEPLKPSSGSQFEPIRHVYEAIQQTSGYVVRAVPDDAK
                     FPIIMFDESGEPAYSALPYGSEIELDSGEAFAIYVDDGDPCISPTRELTIETATADSA
                     GNERFLLKLTQTTSLGVVTTLETHTVSLAEEAKDDMGRLCYLPTALEARSKYLRAVVN
                     EELISTAKVTNKKSLAFTGGTNGDQSKISTAAYLRAVKVLNNAPYMYTAVLGLGCYDN
                     AAITALGNICSDRLIDGFFDVKPTLTYTEALSAVEDTGLLGTDYVSCAVYHYPFSCKD
                     KWTQSRVVFGLSGVAYAAKARGVKKNSDVGGWHYSPAGEERAVIARASIQPLYPEDTP
                     DEEAMVKGRLNKVSVGTSGQMIIDDALTCCTQDNYLHFQHVPSLMNAISRFFVQLARQ
                     MKHSPDGITAAGLTKGMTKLLDRFVASGALVAPRDPDADGTEPYVLKVTQAEFDKWEV
                     VWACCPTGVARRIQGVPLLIK"
     gene            61874..62689
                     /locus_tag="ACHKIK_000618"
     CDS             61874..62689
                     /locus_tag="ACHKIK_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081849.1"
                     /translation="MSKNFFQSGAFLGNGLSRFALNSDPVQLMESARASAEQPTDPVI
                     NNNPEPAAQTNDNVPSAPAPEQILEGKDGKEWTVEQAHQMILEAANRSAMQNALSDAA
                     DAVFAWADSGDLTFDSLDGFVQAIAGISDDDDSEVTEEQDDAYNEAWANVADFLAACG
                     VDDDLIEALADDEDDDAAADVGASIAGLDSDDRDELEAAFVVAGTSDEMLTEAFKKVV
                     RNGEIKLIRKRLRKKRLTAAQKSALKKARRKAQTGAAKLARKKSMKLRRKRLG"
     gene            62842..63306
                     /locus_tag="ACHKIK_000619"
     CDS             62842..63306
                     /locus_tag="ACHKIK_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9081850.1"
                     /translation="MNMAQNWEAPFTGMSMGSVSGALGGFVQVGTETTSVARWNSLMV
                     WEGGTPPTFTLPVTFIALNNPFIEVSGAIAALTAMISPELKAANVGGRIPERVTLNIG
                     RRINITDVAIQDLSFDLDAPRDSNGYFLKNTVNLQLTGSSIYNSSDIVRAFQ"
     gene            63318..63827
                     /locus_tag="ACHKIK_000620"
     CDS             63318..63827
                     /locus_tag="ACHKIK_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HFO9081851.1"
                     /translation="MGHNNTKGNRKFIKGRYTANAAKGERLVSSEFQLTVAGHEDISV
                     LVRTSQIPEMTREDVEDYGPNGVKFNQHGPIRNSGEIQVQCVETIEGDILQFIKDRIA
                     AKDYVDITMAATPESKSSGVNAVTKAATTIEMLDCKIYSDAIDFSTEDVTAAVRPSLR
                     IVYNWIEWD"
BASE COUNT        16610 a        16083 c        14755 g        17213 t
ORIGIN      
        1 cgggaggtgt cactaattta gataagttaa aaagacttct ttaagagata atgactaggc
       61 gctttaactg ctagctaaca taataattgg tcatttaacg tggagttccc cgccaactga
      121 cggggttttt attagtcact ttctgtttct tctggaatgt tttcttgcgg ttctgccaat
      181 gtagcacgac ataggttcct ggcattggct atagccacac ttttgacttc atccgtcatc
      241 gtgcaggtaa tgtactgatc gagctcttca gctcggatga tgcttttgcc aattagaaac
      301 tgtatttgcc agagcagatc ggcatccata atcagaattt ctgccgggcc ttcagggcca
      361 gccgggaagg aaacataaga ctgtttgcct aggccgataa ctcgacaact tgcttcaaga
      421 attgcgcgct tgaggtctga ctttataacg gaaacaggtt gattttcacc agtgattacg
      481 ccgttgacat ggaaaggcat gtagcttgaa atacgctcca ctttccacac gccagcaagc
      541 gatccttcat gcagcacaat gggggtaacc gcgagtttca cctcaccata taactgctgg
      601 cagatagctg gattgctgaa tacatctaaa ggctcacatt caaacagcgg cgcaatctgc
      661 atgaggtcca tcatggtcat ccctggggta cgagcagtaa tgaatttgcg cataccagta
      721 tccattgcgc tccagattgc tacaccatgc tttttgctca cttcttcagt aaagccaagg
      781 tggcacatga tggttttttc gatagccaga tcggagatag aaaccttttc gccaggtaca
      841 ccatcattat tgatggtcac ttcgacactc tggccattac gcaggcggta ttgaattgtt
      901 ttagtatttt gtgctgccat agtcttttct ctgcttaaaa actgatgtat tgcgccttca
      961 ggtgggtcag gaatgttttc ccaccagcga aagcaatatc tcggggtgtt ctgttcgtta
     1021 aaaacgcgtt ccattgccaa ctttggcgtt ttttagcgag ttcgtgcttt tgttggcgtt
     1081 tggaccaccg cttttctttc agtcgttttt tactcataaa ctctaaaacg gaatatcgtc
     1141 ttcaaagtcc attggaggtt cgttattggc gctgctctga ggtttgctac caccgctgta
     1201 ttgctggtgg ttttgaggtt ggtttgactg cccccagcca tttgaggact gtgaatcgtc
     1261 gcggcgagcg ccgatcattt gcatggtgcc gccctggctg acgataattt ccgtcgtgta
     1321 acgttctaca ccggcgtcat ctgtccactt acgggtttta agtttccctt cgatgtagac
     1381 ctgagaacct tttcgtaaat actcactcgc aatttcagca atttttccga acaaaacgac
     1441 tttatgccat tctgtttgct ctttctgttg gcctgtttgc ttgtcgcgcc atgattcatt
     1501 cgttgcgatg ctgagtcttc cgactgctcc gccatttggt atatacctga tatctgggtc
     1561 ttgtcccagg gtacctatca ggatgacttt atttacaccg cgttgtgcca cttatctcac
     1621 ctaataaaat aaattaatta gagcaataat gtatatcttt gaaacgtagc taacaagtga
     1681 tttgcattat cctgtgcctt ctaaagggat cgagtcagtc ggtattggct gtgaatgggt
     1741 gtttgtcctg gagcgtaaaa aattcgctta tgaggtcttt atgaagggaa aaacagccgc
     1801 aggaggcggt gcaatttgcg ctatcgcggt gatgattacc atcgtgatgg gtaatggcaa
     1861 tgtgcgaacc aaccaggcgg ggcttgagct gattggtaac gctgaaggtt gccgacgtga
     1921 tccatacatg tgcccggcgg gtgtatggac tgacgggatt ggtaatacac acggggtaac
     1981 gccaggcgtg cgaaaaaccg accagcaaat cgccgctgat tgggaaaaga atatcctgat
     2041 cgctgaacgc tgtattaatc agcactttcg gggcaaagac atgcccgata atgccttcag
     2101 tgcaatgaca agcgcggcat tcaatatggg atgcaatagc ttacggacct actacagcaa
     2161 agcgcgaggc atgcgagtcg aaacgtccat ccacaagtgg gcgcagaaag gggaatgggt
     2221 gaatatgtgt aaccatctcc ctgattttgt gaacagtaac ggcgtgcccc tgcgaggttt
     2281 aaagattcgc cgtgaaaaag aacgccagct ttgcctgacg gggctgatca atgaataaac
     2341 tccggcagct ccgccgactt tcgacaatga agttatcgct ggcggcgata gttttcgact
     2401 cgattttcat ggcggtatat gtgctcaatg agacgtggcc actggaaccg ctattatatg
     2461 ccgggcttcg gctgtgcctg acatttttga gcatggctgc aagattgatg cagcagaaag
     2521 aaaccgcttc agattgccca cgtcgcgcgg tgcgcaaata tatggcacgc agacgaaggc
     2581 gataatagtt aacgataacc ccggcagccg ccggggttat ttttgatggt tatttaaacg
     2641 ggttgattga attattaaac gtgatgatgc ttgtctcacg cggtgcctgg acgttagccg
     2701 cttgcggaac ctccttaatt ttcttggtga caggcaagtt gcgtgcgcca actttgatca
     2761 gagattcgaa aagcgtggcc acgatttttg catcaccagg ttctttgagg cggaatgcgt
     2821 ctttttgggc ggcggagacg aaaatcggga ggttatccag ttcgtcttgc atcgctgcca
     2881 gcacatcgtc gcggataccc gctgttttct ccagcaaagc gattcgcgct tcagcatctg
     2941 cgatcttggc cattgcttcg aggtggcggc cctggctttc gagtagtgcg gtttccagtt
     3001 cagccgtacg ctctgtcgcc tccaccatca tttccagttc agccatttta ctgtaatggg
     3061 atataacggc ctgcactgac tcgtcagagt atccatgcgc cgccagggac tctgccagta
     3121 gagatttaga atccgcgctt tcaaacattc cggcgctggc aggatgatcc agactgatat
     3181 agttcggcgt tgtcacataa tccacaccat ggaagctggt ggttacagcg attttcccgg
     3241 actcacgccc gccagtggcc cagctccagc caccagctcg gctttcgatc atcgcggcga
     3301 caattttacc cggctctgtg ttaagaattt cctgtgtatg ggtaacgatg ccgttgtcgt
     3361 caacagatat agccactgtt cggcacgctg gaacattgtc gattacgacc gggcgacctt
     3421 ccaccatgat cacgctggtt tctggtactt ccagtttgcc ggtcagctgt cggcgaccgt
     3481 gaccgtaata gccgaaaagc tcaccaaggc gtaaaccttc ctgagtttcc ttgctttcaa
     3541 gcatggtctt taccgcgctt aatacatact gtcgcccgtt ctggcgacct tttcgagcat
     3601 tgctatagag acaaaagcgg tcagtgaccg ttttcaaaac atcagtcatt atcgtttccc
     3661 tctttaaaga ccgattcaag gatttgcgcc agttcctgtg gcggtgtttt gatgatggaa
     3721 tccatcaggt gatcgtcgtc ctcgctttta gctttcagtt cgttcaccag tgcttcagag
     3781 attttttcgt caatctccag cacatcgctg aacaggtaac gtttgaatgc atcggaatta
     3841 gcgaggacgc tgttattgct gacggcatcg aggatttgcg taacgatggt ggcgtagttc
     3901 gcctgcgagt cgcggttatc gttgtgctct tgttgcagag cggtattaac ggagtggaat
     3961 tcgattttgt acgggcgatc accttccggg tataccttgc cgtacttgaa agcaagatga
     4021 atatcgatag cccgctgaat gaactcttct acgccctgct ggatccatga ggcgcgcatg
     4081 gcggcctgaa tcgccgtgcg caggaatcca ccttcaccaa gcccgccgga catttgatct
     4141 gcccacccca ggagggtgta atcgaggcca agtgctgccg ccagctggcg catataggtg
     4201 agaatgtctt caatgccgtt gatgtcagcc tggatggtct gagtatcaat agtcatctgt
     4261 cccttgccgt cgcccataat aggcagcaag gtgttggtga ccgtaggcat gttattcgcg
     4321 ccgcgtgcgc gcttttccat caggtcagct gctcgtttaa gcgtctgagt aatggtgcgt
     4381 gaataatcgg ctgcttttac cggatccaga ctattcatcg ccagaccgat gattcggtca
     4441 attttcgacg cattaaaacg cgttgccttc agcgagcgga tcgccgaacg cagattcatg
     4501 tacggctcgt aggcgtattc gagcaagctg gtcccgtaat tctgggtttc aatcggcgtg
     4561 cgctcttccg gattatccag caggctgtaa gccttatggc cagtgtgcac aggcataagg
     4621 tttgacttag gccgccagta ggggattttc atagggataa tggcccacgg atcggcgaaa
     4681 accattttcc ctgacgcgtc cttcagataa tcgccgctaa atcccgccag gttaccgctg
     4741 acctcgaact ctttgatgaa gcccggaagg gtgtaatagg agcactcaaa agacgtgatc
     4801 cctatgcctt ctttggcgta tggcctgaca taagccaccc caaatacaga catgataaat
     4861 gcccacccgg cgacctcttt gttgatggtt cgcccgatgt cgttcatcag ctcgtcacac
     4921 aacccctgcg cggcgtcata gtcactatcg tttccgttgt gtaccggcac gatagagaag
     4981 gtttgtccgg tcttcttatc gaaagagagc gcgtgcgtaa tatggatgtt cagcgcggtg
     5041 gcgatcgtgc tgtaaaccgc catctcttcg agtagaggat agcgttgcaa gcggtcttcc
     5101 ggcagttgaa cttcatcaaa gataaagcga cttccgtcca ccagcccatc gccagccatg
     5161 ccactatcgc ccggtttgcc gcctaagaag ccggacagtt gtaccggtgc ccctgcgcga
     5221 gaaaacaaat acccacttcc gccgtgcaca gccagcgcgg acaggaggat gttgtcccgt
     5281 tctccgttgt ctttaaaaac ccccgccagc gccttcctga ccgaggatag cgtgatttta
     5341 ttgtctgcca agattgcacc ttaattagaa caattcacat catgtttgaa cggaatttaa
     5401 cactagtcac ttgttaagga ttaccaatga acaagctatc tatgtgggtg tttcgctgtt
     5461 caagtgtcag cgaaatattg aaatatatta gtgcaataac atttcatcgg gcgccgatta
     5521 gatacggcgt ggaaaaggtg gaaggcaaaa gctatgaccg actacgccgg acgtgaaata
     5581 atatgtccac actatctgat acaataaaac cgaataggac ataatttcga cgtcaaagcc
     5641 ttggcacagg ctggagagaa aacttgaaga agcgatacta cacagtaaag catgggacgc
     5701 tacgagcatt gcaagagttt gctgataagc ataacgttga ggtgcgcagg gaagggggaa
     5761 gtaaagctct gcgcatgtac cgtcctgacg gaaaatggcg tacggtcgtc gatttcaaaa
     5821 caaacagtgt tccccagggc gtccgcgacc gggcattcga agaatgggag cagatcatca
     5881 tagataacgc attgctcctg aatgcagatt gacaattttg cccggaatta ccgggcaaaa
     5941 ttaagcaact atccttcgtt acgaagatgc ttatctaaga attttgtaaa ctggtttgtt
     6001 gagccaaaat agttggggta ggcttttttt agtgacttaa tgttatctgt agcaacgaga
     6061 acagatatca atacattagg atcagaatcg tctttattta aggcctcaag ggcttcatct
     6121 gcttcttttg ttttaaatgc gcgaacagac actaggaaca tttttttgtt ttttgtattg
     6181 ggcttgtttt ctgcatttct cattgttact aagtaatgcc ccttatgatt ttttggcaac
     6241 ttcttaatca aattcgcgtc agaagttaaa tttaaggcga gggtgtatgt gcttagtttc
     6301 ctccgtacag ataaagtccg ttccagctct ttaagctcgc cttcatactg agatacaacc
     6361 agctcatcaa gagtgcaggc tttttcgtcg tgcgctacaa gacatccggc tagatagaaa
     6421 aatcgtctcc attctggatg cccttcactt gaagttttca gcttgatacc ttcaagggta
     6481 tcaattattt caaggctagt agcccaagca tgctgcaatt gagttcttaa ctgtatctca
     6541 atctttgttt ttcgccacgg atattgctcc tctttctgat cgaagcaact gtatgcaagg
     6601 tggattccac tatagccgct gtctttggga gtaagataat catactcttt aacaatccta
     6661 tgaacagatt tgctttttaa aagacgttcc tttagctgta tcaactggtc taagtttctg
     6721 acaatggccc tacatccgcc aacgtcctgc atccgtgtta atttaattgc attgctagtt
     6781 ctgcctccat caagactagg acgctctagt ttatctatta tggtagaaag tcgcttaagt
     6841 cttcttgcaa cgagaatctt tttgtctttg tcaaccttag cagctgctct tgctaaatga
     6901 tttttcatga gcattaatgg ataaagatgc agctcacgga aattttgtat catttcaata
     6961 gcttgctgcc tttcttcgtc actacacccg tgccttatca attgagcagc cctatcaatt
     7021 tgagattttg aatatttcaa cttgcatttt tgactttgat aaacttcatt ccccattgct
     7081 ttcatccatt ttaaatgatt tacatgctgt tacatctaat gataaagcgg ttagaaaatc
     7141 aatggagaat ataaccacaa aattactgta tataattgtt aaaaataatt atgcttgcct
     7201 gttaataccc ctttcggcac ttttttttgt tccagtatct gggtgcccag aattatgtta
     7261 atcagtggac ttagtaacga tggttcttgg cgggcctcaa cttctccagc cattgccctg
     7321 atgtagtcgc cgctggcaac gttgttgtat tccgtcgcaa agcaacatag taacgtcaga
     7381 acatgctctg tcgttatttc gctccagttg atgttgaaaa attcatcgcc ttttttatcg
     7441 tgttcggaat cgaagatgct ttggtggaga atgtatttgc cggattcctt gcgcggtaac
     7501 ttgatcgctt tctggcgttc cagctccttg taaatctgca ttgcttcaat cagtaccggc
     7561 ctgccgttca tgaagggatc gcgcaacctt acacgctggc caactcgacc agtaataaag
     7621 ctgttttcct cttccaccag cacgataaaa cccttttcct ctttttctcg caattcgcgc
     7681 agcagctgga gttccatatc gcggcggcgt tcagggtagc tggtacgctc agccattatt
     7741 agctcattgt tgatccatgc ggcagtcatt gacgccggtt tgccgacgct catcgaaaca
     7801 acgcatattt tcttatccat agcgccccta caaaaaagaa aagccaccag cggcggctta
     7861 gcaatacaac tgaaggtagc gcccggtact cagactgtgc cgtccatgga atatttgaaa
     7921 agggatccat ccgtaccggg catgtgatga ttctgactga agtcacttgt cagttgtcaa
     7981 tcatttatta ttaaaaataa tatatttatt agtgcataca aataagacac taagtgtgca
     8041 cattgcaacc tcagagagga aacattcaac tagcatgcaa ttgctctaat gaatttgatt
     8101 ttttggggaa gaaatactac cattgtacgc cagactattg atcttcctgt cgttcaggct
     8161 tatagttacg ccgtcgtagc aaattctgcg accgggttta gcagcctgaa tgtacatgcg
     8221 gacaaccgca gatttccgat attgcggtat ttttatgtcc gtaaaaccgc gttacgcccg
     8281 aattatggtg gggcgtgatg gggaggcttc ggcctgctgg tttcatgtac gccagtctgc
     8341 taaccccgtc acgtcctgcc acccgtttag cagcgggtag caggttgtta aacctgtaca
     8401 tgaggccgta actatggtta atgccaatcc ttgcgcacgc cctgaattta tctggcgctt
     8461 ttactcctgc cagaaacgtc actatcactt cgttattgca ccgacagaag atgaggctcg
     8521 ctctcagctt ccggatgctc cctgcatttt ctctgcccgc ttttctaccg attcgcgcaa
     8581 ttctctcagt tactggtgcc tccctgttaa cgcttctgct caggagggac tatgagaacg
     8641 tcattagtca cccgtgaaga aatgatcgag gcaattgaac agcacacaat ctgtatcagc
     8701 accagggata taccaggcgt tattgccaac tacttcatga tcaccaaaca actttaccgg
     8761 agaaaggaca agaacgcggt tcaccgtatc ctgttgtctg atatccgcga atacctgctc
     8821 gaacagggtc atctgaatta cgcaaccgtc gcagccgaag cacgcaagga ggcacacaga
     8881 atgaaagcaa ctaacgttaa atcagaaaaa attcatgcaa cttcagttca ggaatcggag
     8941 ctggtggttg ttcagaatca gtctgatgaa attcccgttc tggaatggct gggagtgcgt
     9001 gtagtgacaa ctgagactct tgctaaaggg tatgggaccg atgaagcgaa tattcgtaaa
     9061 aatttgtctc gcaaccccga tcgctttgaa gaaggtaagc attactatct cttaactggt
     9121 tcaaaattaa aggaatttaa aaggctagtg actattagtc acttggttag caaatataca
     9181 agtcaggtaa ttctctgggc cgagcgcgga gcggcacgca tgtctaagat cgtggacaca
     9241 aatgaagcgt gggcattctt tgaaaaactg gaagacagct acttccggca aaaagaacag
     9301 caaccggtcg caatccctca gacgcttccc gaagctctgc gcctggctgc cgagctggct
     9361 gaacaaaagc aacttctgga acagaaagcc caccagctaa atcagcagct ggtggccgcc
     9421 gcccctaagg tcgattttgc cgaccgggta tcagtggcta aggggatcct gattgggaat
     9481 tttgcaaagg ttgttggact taagcaaaat gcgctgtttg cctggttacg ggagaacggc
     9541 atcctgattg cgtccggcgg acgtaaaaat gtaccgtttc agcagtacat aaacgccgga
     9601 tatttcacag tgaaagaagt ggtgctggat gatgaagatg gctatcagat acggctgacg
     9661 cctcaattaa cggggaaagg ccaacagtgg ttgacgcgta aactgctcga tgctggcttg
     9721 ttaaaaccgg tggcggctga atgattaaaa aaggcggcct ttcggccgcc aatgatgtca
     9781 cggagtctat aatggcaatg tcttcgtagt tgactaaagc cacaactcaa ttataacaat
     9841 taattatagc aatagtggct attttattta tcgcgaatca cattttttct cttcagtacc
     9901 tgtgtgctat actccttctt gattgattgt atgcggaata caaacccgct cttttgtgca
     9961 gcctggctcc ttgccaggct tttttatttc atcatggaag ctgttaacgc tttggacctt
    10021 gctgaactga ttgagagggc tttgtcaacg tgccacaaaa attcgccaaa ctcagacatc
    10081 actttagcaa aaccgcgccg tgcttcttcc tcggaggcgt taatcacgaa atgttcagca
    10141 ccacgcatac ttttgacagg gaacgcaacg gatattgagt caatatcagg cattctatcg
    10201 ctcagcttta cagtgacaat gacggctggc gactgaatat tagtgcttac agacagcact
    10261 acatattttc cgtcgatgtt gaaatccttt ctcatatgtc accataaata tcaatgaatt
    10321 agagcaatca tttacgcgtt aatggctaat cgccatcttc cagcaggcgc accattgccc
    10381 ccgtttcact atccaggtta cgaatgtagt tcatgacaat atttacgttg gtccagccac
    10441 cagcttgcat gatctccggt attgaaactc cggcgcgggc catatctcgc gcggcaccga
    10501 cacgggcact atgtccagac caggccaggt atcgctgacc agagtcatct tttgccccgt
    10561 aaatcaatcg gtgagttgct tcaaaaatcc cttccagggc gcgagttgat agctggctgg
    10621 tggatgatgg cgcggcaaca ccattttttc tgacgcggca aaacaggtag ttattcggat
    10681 catcagctac accagagaca gaaatccatc gctcaaccag tttagttacc cccaggctaa
    10741 gtgccttctc tacaccagcg gtgctaacca gcgttttcgt tctgccaata tggattaaca
    10801 ttctcccacc gtcagtacgt gagatatctt taaccctgat cctagaaatt tcggctatac
    10861 gtaacagggt gttataagca atccccagaa atgccagatt acgtatatcc tggcagcgat
    10921 cgctattttc catgagtgaa cgaacctggt cgaaatcagt gcgttcgaac gccagcgcct
    10981 gttttgcacg ctcaccggca tcaacgtttt cttttcggat ccgtcgcatg accagtgaaa
    11041 cagcattgct gtcacttggt cgtggcagcc cggaccgacg atgaagcatg tttagctggc
    11101 ccaaatgttg ctggatagtt tttactgcca gaccacgcgc ctgaagatat agaaggtaat
    11161 cgcgaacatc ttcaggttct gcgggaaacc atttccggtt attcaacttg caccatgccg
    11221 cccacgaccg gcaaacggac agaagcattt tccaggtatg atcagaaaac gcctggcgat
    11281 ccctgaacat gtccatcagg ttcttgcgaa cctcatcact cgttgcatcg accggcaatg
    11341 caggcaaatt ttggtgaacg gttaacgaat tagtcattta ttatgcgtga ttttaacagt
    11401 taagccttgt tcatgaagta tctcatcaat aattcgaatc acatcccctg gtagatcatc
    11461 gttctcgaaa tatctgtgat cggggatttc tacagtgatt ggggttgctg ctttatgcaa
    11521 aatccgaaca atcttttttt cttttttgca tgttgcatat tcagcctctt cgatatctat
    11581 ccagctcgca ccatcaaatt gctggtaaac ccgagtgtat agtaagcggt ttcgctttac
    11641 ttcttctgct aactcccaaa ttgcaacagc catagcacgt tcattttcat tcgttccagg
    11701 gttagcagct ttttgttttg cgtcgttcag taaggcgttt accttggcat ctgtcaggcc
    11761 attaaacatt catcacctca tgatcaagtt aaattattgt tttcagcaga gtgtacagga
    11821 ttggctctgc ctttacctgg ttatggttct cgtcatagaa acgccagcga ccgcgcgtgc
    11881 gttctatttt ctcttcaccg cgagataatg acagttggta actatcacgc tcaaaccatt
    11941 ttgcccgcca gtaaccacgg tttttctcaa gctcaagatg agtggacact ttagcagctg
    12001 aatatcccat ttttcacctc tgattgattg gtggtgctaa gtgcgctacg cgaaatctgg
    12061 agcactaaca ctgccaacat ttcgcagatt ttacgtagcg caaccttgat caaatgatca
    12121 agtgatcact atttgacctg ataaggtatt gaactgtatg gatttacagg taaattaatc
    12181 atgttcaata acccttaaga taacttcgta taatgtatgc tatacgaagt tatcaggtcc
    12241 gaagaggagt ttacgtccag ccgcgcacaa aaatcaagaa ttattagagc aataaatttt
    12301 gagagaaaaa tcccactcca ccagctaaaa actggattgt ttttcatagt tgtttgacaa
    12361 ttgctctaat aaattatagt tttgccgccg ttacgtaata cgactttgga ttcactattt
    12421 aatgtgtctt cagcgttgta gagcggctca gaaggaaatg agcaaacagg gaaaccttat
    12481 acaacggcat tacagctatg cattgctcat cttacacaca gcgcaatgtt gttagattac
    12541 cccagcatgg atcatgggtg aaacagtagg tcagagcttc aggctctgtg ttgtcaatac
    12601 agtgaggcat aattatggct ttcattcaac caaccatcga cgacgttaga cattgctcta
    12661 acgctttatc tgtagaccct gccgaaaccg acgctgcccg cgccattgct gaacactact
    12721 caaagatatc caatcaggag taccgcatca cccaagacga cctggatgac ctcactgaca
    12781 caatcgaata tcttatggca actaaccagt tagactcaca ataaatgcac taataaatct
    12841 attatttttg ttttatccct ctataatata ggtcagtaat gaccggtttt ctcagccggg
    12901 cgttattgac catgtcaatt ctggaggagg atcaatgata aattatgtct acggcgaaca
    12961 actgtaccag gagttcgtca gcttcaggga tctctttcta aaaaaagctg ttgcacgcgc
    13021 ccaacacgtt gatacagcca gcgacggtcg tcctgtacgc ccggttgtcg ttctaccgtt
    13081 caaagaaact gacagcattc aggctgaaat tgataaatgg actttaatgg cgcgggaact
    13141 ggaacagtac ccagacctca atatcccaaa gactatttta tatccagtgc ctaacatcct
    13201 tcgcggtgtg cgcaaggtta caacttatca gacagaagct gtgaacagcg tcaacatgac
    13261 cgctggccgc attattcatc tgattgataa ggacattcgc atccaaaaaa gcgcggggat
    13321 caatgagcac agtgcgaaat acatagagaa cctggaagca acaaaagagc taatgaagca
    13381 gtacccggag gatgaaaaat tccgtatgcg tgtacacggc tttagcgaaa caatgctgcg
    13441 cgtccattac atttccagta gccctaacta caatgatggt aaatcagtta gttaccatgt
    13501 gccgctgtgt ggtgtgttta tctgcgatga aactctccgt gatggaatca tcatcaacgg
    13561 tgaattcgag aaagcaaaat ttagccttta tgactccata gaaccgatca tctgcgaccg
    13621 ctggccgcaa gcaaaaatat atcgcctggc agatattgaa aatgtaaaaa aacaaattgc
    13681 catcactcgc gaagagaaaa aggttaagtc agccgcatca gttacgcgca gccgtaaaac
    13741 caagaagggg cagccagtaa acgacaaccc cgaaagcgcg caataaatta tgcccggcat
    13801 caaccgggca ttcttccatt attcagccgc caccggtttt aacaagccag catcgagcag
    13861 tttacgcgtc aaccactgct ggcctttacc cgttaattga ggcgtcaacc gtatctggta
    13921 gccatcttca tcatccagca ccacttcttt caccgtgaaa tacccggcgt tgatgtattg
    13981 ctggaacggc acatttttac gtccaccgga cgctatcagg atgccgttct cccgtaacca
    14041 ggcaaacagc gcgttttgct taagtccaac gacctttgca aaattcccaa tcaggatccc
    14101 tttagctact gatacccggt cggcaaaatc gactttagga gcggcggcca ccagctgctg
    14161 atttagctgg tgggctttct gttccagaag ttgcttttgt tcagccagct cggcagccag
    14221 gcgcagagct tctggaagcg tctgggggat tgcgaccggt tgctgttctt tttgtcggaa
    14281 gtagctgtct tccagttttt caaagaatgc ccatgcttca tttgtgtcca cgatcttaga
    14341 catgcgtgca gcgccgcgct ctgtccagag tgtaaggctt ctggcgttct taccaacaga
    14401 gtaacttccg gttactctgt tcttaaattc tcttaatttt aaacccgtta gaagaaagta
    14461 atgcttacct tcttcaaagc ggtcaaggtt gcgagacaaa ttgttgcgaa tattggcttc
    14521 atcgacccca taccctctag caagagtttc ggttgtcacg acacgtactc cctgccattc
    14581 cagaacggga atttcatccg gctgattctg aacaaccacc agttccgatt cctgaactga
    14641 aggtgcataa attttttctg atttaacgtt agttgctttc attctgtgtg cctccttgcg
    14701 tgcttcggct gcgacggttg cgtaattcag atgaccctgt tcgagcaggt attcacggat
    14761 atcggttagc agaatgcggt gaaccgcgtg tttgtccttt ctccggtaaa gttgtttggt
    14821 gatcatgaag tagttggcaa taacgcccgg tatatccctg gtactgatac aggcagtgtg
    14881 ctgttcaatt gcctcgatca tctcttcacg ggtgactaat gacgttctca tagtccctcc
    14941 tgagcagaag cgttaacagg gaggcaccag taacttagag aattgcgcga atcggtagaa
    15001 aagcgggcag agaaaataca gggagcatcc ggaagctgag aacgtgcctc atcttctgtc
    15061 ggtgcaataa cgaagtgata gtgacgtttt tggcaggagt aaaagcgcca gataaattca
    15121 gaatgagcag gggtagggat agtagccaca atggcagcct ccttttgcta atttaaggag
    15181 ctaccgcgtg aggtctcaat ctcaatggcg gtagcactga ctgggttgag actaccggcg
    15241 caaaagggaa ccggcctgcc tttcggcagc ccagccagca ctaccattga tctctgagct
    15301 aaacgctacg tatggctgtg cgatggcatg acacaaaaaa agacgctttt ggcgtctgtg
    15361 tcgccttttg cattatccgg ggtctcaatc ccggcacccg ttttaatgag gtgcctgata
    15421 agcataaacc gaaaatgcct caaggcgcaa gaggtcaggt tcaatgtaac atcggtagtt
    15481 aaaaaacaca atttattaga gcaaatattc attcattaag ccatgccaga gcttcatcaa
    15541 cctgcgcttc gtcttcgacg ctaagcactt catcttgggg aacatagttc gccaacatag
    15601 cgaaacaata tgtatcccaa tggtccggtg agtgcaggtt gagttttttc ttcatatctt
    15661 cctttgacat caccttccat tgacctgcgg aatttatccc taccggtatc tttgatgctt
    15721 cctctatagt cgcagccccc ttatcaagcc gcatacgccc tgattttaca gcttctgccg
    15781 cctgaatatt cgcgaaagcg cgcatatcga aataaaggct tttatcttca cggctgtgca
    15841 tctttttacc ccagcggata cgctggacgg taataccata gcgttcgtac atcagatcag
    15901 ccgtcgattt ccccaagcca tcgccatcaa tagctatggt tatgttcggg aaccgttctg
    15961 ggttacattc tgcgaaaatc ttggcggcta actgcgtttc tgtaacgtct gtgtattcca
    16021 gcatacgata gttgattaca cggcgtttat ttcgctggcc ggacaccatc atgatattaa
    16081 taacggactt atctcgtcct gtgccaccag caacgtcaac acatgcaacc cagccccatc
    16141 ctttggcaat cttgaccttt cgccgcgtcg cccgctcaac ctcatcacga ccaagaagaa
    16201 agccatcttg agatttggga aattcaccac gtactttgat catgtacatg gggttatcac
    16261 gaccgccata ctccgcaagt tttgctcgta taaattttgc atctacaagc ggagattctt
    16321 cactattcag tattatcgca gtaaacaatc catcaggatt tcccgggcga atagctagtc
    16381 tgtggtgtga atcgtagaaa tagcctgaag gtcgcgtagg ctgggaaaga agcagaatac
    16441 ggttatcctt accggtcagc gcacctgtta tcacactgaa tgctttatca ctcacacccg
    16501 acgcttcgtc gatgatatac aagagatgat cggcgtgttc accagccaac gcctcctcat
    16561 ttccggagcg acaggacttt atcaatattg tccaaacacc cttgccagtc acctcaaaaa
    16621 aagacgtttc tgtaagaatg aaatacttcg acaaccacgg gaatctgcta acagcagtag
    16681 cccaattgct ctttatgtat ttgaaaatac catcaaggac ttgctgtctt ttgttagcga
    16741 ccagaataac gcgagcgccg gggaaaaaca tgatgaagag tattgcaatg atactcgtca
    16801 tatccgactt accagtacca tggccggagg tcacacttgt ccaactgccg tcctgctgcg
    16861 tggactcaat gatctcatcc tgctgccagg ttggtgtctt cccaaacaac acatcagcgg
    16921 ccgcaatcca gtcataacga tatagcgcca ccagctcgcg ccaacgtggg tccgttacgc
    16981 aacttctggc cattaatcat catccccgta cagtttgcgg gtaacttctt cgtcttcctc
    17041 ctcgttttcg tccagatcct gttcaagcca tgcttcgttt gatatgcctt ccgcatcgac
    17101 atcaccataa ccacctgtat cgacgatatc ggcaatttct tctctacgat gctcaatcca
    17161 caatgcggca tcagcgcggc ggctggcggc ccgttctcgc gcaactttgt ccagatcttc
    17221 aagagaaggg ccaccgacgg ctgtttgcct ttcctcatca tcggtattgg tcttaggagc
    17281 acgcagatcg gctttgattt gctccagcat caggggcgga actttcccac catgcgcctc
    17341 gatgaattca gctgcttcca gcactgacca gttattttca cgctttcgtt cgtatgccag
    17401 cttaacaatg ccagcttgcc ccatagataa agcgtgcttt tccgcctccc ggctttcttt
    17461 tcgatagtta ttccggatgc tgtaaatggt gttgatcaga ctgcttatct gcgcggaaca
    17521 gctgtttagc atgctcgcga tacggtattc aggcggagta ccttcatcat cgtctttttg
    17581 ctgatcgcgc atttcctgca ccaggcgaat acacgtatcc ctggcgttct ccagcataag
    17641 gagatgagaa agagactttt caagaagagt ggtttccaga acatcggccc cggaccgacg
    17701 caacatagcg cgcgcggcct tccgcgcttc aacgttatct atcaggtaat cgccagcttc
    17761 gaattcaaag cgttcaccat catcatccag ggtgtcgcgt tccaggcgat cacgtaaggt
    17821 ccggtgggcg cgggtgatca cgtcatgatc atctgaacga tcatttatgc gcttattttg
    17881 gcgcttcgca ttctcgactg cggcactgat aacggcatta actctctgtt tttccgctat
    17941 tccagccgca atatgatcac ctgcatgttg atcattagag tgatcaatga tcatgctttt
    18001 tagtggcttc ctgactggct tatttggctt gcggctgtcc gcagtcctgg agtcttcttt
    18061 gaaggcacgg agataacgac gtgcggtatt agggttaaga ttaaactcgg cggcatactg
    18121 tgcgatggtg taaccaccat ctcgcgccag gcgagcaaaa ttcttcttgt gatcgtccca
    18181 ggtcacttat gcttcctttc gtaaaaactc tttttgacgc gagggtaacg aaagtcacat
    18241 gtcaaaaggc ccggaacggg caagcaatca atcagatacg tgcggatgtg gcattaccgt
    18301 aatgacggtg ctgacgggcc accttattga aaagttgacg cgccattacc caaggctggt
    18361 gctcccggcg ttccttttcg tcctgcgtca tatagagttc gttctggagt ttttcatcaa
    18421 accggcgcgg agcgcggcta cggcgaaaga attcaggatt cagagagtgg atctgaaatc
    18481 tacgtgggcg tgtactgtca tcaatcaaaa cagacgaata cttagacaca gcgatagcct
    18541 ttaagcgcag ataaacatcg cgcttatcga catccagatg cgggtattcc ttttcaagga
    18601 ttgctgcgag ttctttcgct gatagaaaag acttagtgcg gatcatgtaa tccgcaatct
    18661 catacgatgt tattcgtgag tgatttattt ccatgaagtg gcgtccctgc cagttaagta
    18721 acatcctgtc acctactgat tagcccatgt caactaatca acgtggaata taataccctc
    18781 tattaaagaa atagcaatac attagagcaa ttttatctaa cgctcgacga gtgacttgtg
    18841 atagcgccga ctccaagcgc gtaatcaaag aacaatcgtt gatgcatcgc cagcctaccg
    18901 tgcgtcttct cccaattatc gcggtcacgc tcaatatcac gctggcatga ctggcacaga
    18961 ggaatagcat aaatgtcatg cgcgcataat cgactatgac gaacgatata aggcgtaatg
    19021 tgagcgccag ctcccgcagc tccacagcca cagcatggac gggaagccac aaagtccatg
    19081 tactcgggca attttagcga ttgaagtttt ggtattttga aatgcgccat atctgggtcg
    19141 gagtcaacat ccacagggca tacttttgca cgcatcggcg cggcgcgttc ttccatcatc
    19201 tgaacatatg ctgtagcgcg atcgtcatac gggcgaatat ccgcctcttt cagaggtccg
    19261 ctatcctgcg gagtagcctt catcttattt attgatatgc ggcagacttc ttccggcatc
    19321 aggtgcatca tgttgcgcat gaaagcccac cagcacagct cctgaatact taaatcatgg
    19381 ctatttgaaa ggcccatttc ctgacgggcg acatccagta tccagttaac gcgattattg
    19441 tgcagcgttt ctttcagctc attaaaacca cgcatccgat aatggttatc gtgatgccag
    19501 cacaacaaca ccgcgctatt gtctcgttca gcgtggacaa tatggttgtc acaccaacta
    19561 cgatctgcgg cctggcattg cccctctttc ctgcgcaacc acgccaccag cgcgtcaatt
    19621 ccaccaatac ggcgaaacag ttcatcgctg ttaaaaaacg gctgcaacgc ctcatttgtt
    19681 gccatagttt gctcggaaac aacgaggccg tcgtccatgt gctcgattaa ctcacgcggc
    19741 accggctcca taataaattt acggccagcc tccaccagct ttctgacttc ctgatccact
    19801 ttgaatgtgg cgacgccaag ctctttttgt acaaagggag taattacggc tttcacatca
    19861 cacctttcat cactgattgg gctttatctg ctgcccggca ttctctgttt aagcacaacc
    19921 atttcctgac ggcataacac agcaatagcg gtcctggcac caatttgctt accaaccagg
    19981 tattgcttta cctcgcggcg actcacgcca tcaagaagca tctttaacgc ttcacgggac
    20041 aatttgttgt atttacgtgc cattaatcta ctccgcggaa ccatacaatc tacgtaacgt
    20101 gtcggcgaca gaagatacag atatctctcc ggtcgcagcc cctacggtaa ggtctgccaa
    20161 ttcaggtgaa tcaaatacct gcaccccgtt acggcgtaga aataacagcg cactgtttag
    20221 cgcggtacgc ttattggcat cattgaatat atgccctctc gctgtagcca ccaggtaggt
    20281 ggcggagact tcgaaaaggt cggtgatctc ttcgtaggca actctggcct gaactctccc
    20341 gataatggcc tctgccccac ccggatctga cattcccggc aggccgccgt agcggtttat
    20401 attcgcatca tgaagcgcaa taagttcttc cggtgatata tgcctcatta tcggttaacc
    20461 agttccttgt tggtggagtc cagggtgtca aacagggatg caaattcagc atccagcgcc
    20521 gcttttttgt aggcttcaaa agtagccttg ctaacaatta ctgctggctc acggcctctg
    20581 cgggtgattt caacctcttc cccggcttca acattgttga gcacttcaga aaggttgcca
    20641 cgcgcggtac ggaagttaat ggattgcata aacacctcat gtactcgtta tgtgtacaca
    20701 attataaact tcacaggtat aaagcaccag cactttgcag cttaaatcac cggacaatca
    20761 tcaaattccc cacttcggat atcattgatg acatgagtga tcacaccaaa aacagcatta
    20821 ctccccgtgc atccatcgtc atccactggt aacgcctctt tcttcccggt gcttaaatcc
    20881 tccaggtgct ggcgcggata cttcctgtat ctctttatgc gatattcacc ctccatagcg
    20941 cacacaagca gagaaccatc aaccggagta agcgaggaat caaccaccag caaagcaccc
    21001 tgcaatattc cctcacggtg atggctatca gctgcccgca tgaagtaggt tgctgatgga
    21061 tgcctgatta gttgctgatc aagagaaatt cggctttcaa cataatccgc cgcaggagaa
    21121 gggaagccca tagcgttttc acctcaataa tactgttaat ttatacagta tacattaaag
    21181 agacaccttt ggtgcaaacg cgttacgtac atcaaccacc gctgatgatt ttgtgctctt
    21241 tgctactatt catcaccgcg ggatcagcgt aacctcgttg ccaatcagtt aataaggaat
    21301 tagctatgcc taatcgcatt cctctcgatc ctgtattgcc caaaaatttt gactgcactc
    21361 ctaacgagaa acgctctaaa gctcagctgg acgcctggtg ggaccatcca tatggggtta
    21421 cagaacatga cgggaaaatt gttgtttatt gtctgaatgg tggcgcgtgg gaccgtccat
    21481 ccgtgcttgg tttggcaaat aactatgatg aagcctgtga acttgccgaa agacagcagg
    21541 caagctgggt aaaaacacgg tctgaaccga cattcatgtt ttcaaaagaa ccgccattta
    21601 tactggcgag gatgccgcag cgaccggatc atcaacaata aattgttgct gaattttcct
    21661 caagggatga gatgaatctc ttctcattaa agcaggaaga aagggatcgc gtcgaagtgt
    21721 ctccaactct cgaccacaac cggatgaacc tggcccaact cgcctggtac agcaaagaat
    21781 tagagatgtc tattgcccga cttgaaaacg aaaaagccgc tatccaagtc cagcatgaag
    21841 tagttctgaa ccggattaga gaaatgcaaa acgataatag aggctaaatc gaggtagaaa
    21901 acacattgtt taaggccact aattgtggcc tcaaagggga gagatgctta ttggctatgg
    21961 ctaaaaatca aatgagacac ttcatgtggt tgtagctcgt caagatccgg ggccacaaaa
    22021 ccttttccga aagacttcac aagtaattca ccagccgcat cattgtcacc tattacacga
    22081 aagtcatacg gcagcatcgc aagttgacgg cgtaatcctg cggagaaagt cgaacctaac
    22141 agcgcccacg aattgccgcc cgcgttgtgt acagctgcgc tcttaaaaat agactcagtt
    22201 atccatattg ggccatcttt taaaggaaga taggttcccc aaagtaactg tctgccatgt
    22261 gatcgcgtga aataccgggc catcttcgga ttgctatgct gcttaggagc acctggtcta
    22321 tatgtctgat acccagccaa catcccatca aacctgtata gagggaatgt aactactcct
    22381 tcactttcat cgatccactg tctatatggg tgtatatcat ggcggtagtt acgagttagc
    22441 aaatgagctt ccagctttcc aaattttgtc atattccgtt cttcctgcat actgggcatg
    22501 tgttctctgt gaaatatggg aaactattat ccagctcatc tacccactgt ttatggtcga
    22561 agcagaaaaa tatctcccac ggcattccat attcttccat ccataggcgg atctgcattt
    22621 ctattggaat cggcggtatt gcagacacca tatcaacagg ataacagttc agacgacgtt
    22681 ccagttcttc tactctcttc tggagatgaa atatccgatc cagatgcaca cgccgctccc
    22741 gtgccatttt aaaataaagc tcgccggtca tcattaactc ctacattcag ggcaagagtt
    22801 cccttccgca aaatagggga aactgctatc aagctcatca caccatctgc gatggtcgta
    22861 gcaccaaaac gcctcccacg gtgcacggaa atacttcatc caccaggaca ctcgatcagg
    22921 tatatctgct ggtggcagct cttctggcaa taatttgcct gataattccc tttccgcccg
    22981 cgccagcatt ccttttagac tcgcattctc tttttcaaga atatctatgc gcgcctgtaa
    23041 ctcagctttc gttggcatgg tccgcctcat gcttttcagc caccagcggc aataaagccc
    23101 tggccatctt atgaaccaac agagcatcga taatgccaag cgtatgcccc ggcttaatgt
    23161 ttaatgccgc ctcaaggtga cacctttcca ggtcactttt ctcggcttgt ttatgatggt
    23221 ctggcgtaat aacgtcgccc aaaacacggc taattctttc tcgtaattgc tgggtgcctg
    23281 cacacttgat cgctgtatcg tggagacggt taaccagttc gcgataaaca tgcggcttaa
    23341 tgcggatacg ttcacctgtg acgccctttc ctggcgctgg caccgaacta tccggaatat
    23401 ccggaatatc cggatagttg ccagcctcgt aagctacccg cagccagtgc atgaatgttt
    23461 cagtggacac acaaccacag tccacatcga tttttccgcg ttgctgttcc agccactgct
    23521 caaaattcaa tctacacgta ttactttcat gttgctcttt ttgtctcaag gccagcacct
    23581 gttgggccag ttccagaacg ataccgggtg acgctaacct ctcaaattcc aataaatagt
    23641 ttgcgtcagg atgacaagta gcctcgcctg caaaaaatac caattgctgt aagtatgctg
    23701 tcgttagagt agtcatttct ttttgcgcca tttcttttca cattccttag tccatttttc
    23761 aatgttcatt ttggcaatat cagtcattcc atcacctaag aaatactttt tccggtacgt
    23821 cttgcactta aaccacacta caacagccac cagccagaaa ataaaaggcc atacggcaat
    23881 accaactcca gccgcgataa agcccaatat ccataaatga agctctccaa cttctgtttc
    23941 cggcaatatt cttaaagaat taaacagcag gctgaacgaa tggtcgtatg cattggcagt
    24001 ataagacatg caatccatat aattaaagtc atagcctgcg gctgccgccc ataatgggcg
    24061 gtcaagaaaa tgttttagtg tcatcatatt aatttaaggt tcaggccagt tatcttcaat
    24121 agcaatgctt aatctttgta gccattctgc taatttcagc attgcttctc tttcgcttaa
    24181 accacacgga aaatcatcaa gcgatattgt tggcttgaag tttcccaaat tatccatttc
    24241 aacggtcaga ttttgctcca gaacggtatt tcttacgcgg ctattgtgcc gaagcaaata
    24301 tactgaacgt gatttattgg ttttgtggtc gaacgtatat tcggtaagta tcatctgact
    24361 tccgccatga ttattaccgc gccacataat tactccgtgt taattgaaat ttagctatta
    24421 atcttcactt ttatcgcgaa cacctttacc ggtttatcac cgaagtgtgg atgtgtgatt
    24481 gtttttattt catatccgtt atacgggacg tcaattctgc gactgaagtc ttcgcgcttc
    24541 ggatatccct ttgtgataat caggcggtca tacttacagt taacgaggcg cttattccag
    24601 tagtcattac acaggcgata ctcttccgtt ttctccccgc gaatcatggc atcgaagtat
    24661 tcacctttaa cggcaagttg caggttagcc attacctcac ctccagtctc cataccgcct
    24721 gaccaatccg gctggcatgg gtatctttgg atactgttcc gtctttagcc agctccataa
    24781 gaattttgcg caaatctgcc gaacgccatt cttcatcagg aaattccttc tccattgcca
    24841 accgcagatt ccaggttgcc atcctgaatg gatattcccc gccgagagct ttatcttgca
    24901 gggcagcccg ggaacgcatc acctgcaaaa ccttctcttt tacatccatc atttcgcctc
    24961 ctgcggcggt tctggtagcg gcatccagaa caaggcgttc cctaaccacg ataaagtgcc
    25021 gtcgctcaac tccacgtatt ccccttgtac ctgtcctgcc atatactcgc cgtgctttga
    25081 ataaattaaa atccaatcat cttgaggtgg cattcgctca ctacagctta tccaaccatc
    25141 cggagttacc ggagagttgc ctgccagtct acgcaaaaca gccttaacag cctcaatacg
    25201 gtcatcatcg caattttcca gcgtatctat gcggtcgagc atgatgatgg cgttatcaat
    25261 atcaggattg ccggtccact cattaccgcg attggattcg gcagcctggt tgccagatgc
    25321 tggctgattg tcggctttac ccagtctgtc gtcgctgcat gaatgccctt ccagccaggc
    25381 caatgcttgt cgcatgaaat acgcaatatg tttgccgtgg taatcgtctt catcgatgtg
    25441 aaaagcgata ctacgaatgt attcaattgc gttttcaatg gcctctaacg ctatcggcgc
    25501 tggcggagtg gtatatagtt ttcgacattt gtatatccaa ccggcatggt caggcgtgtc
    25561 tgtaaaacgc aaatcgtctt cgtagcactc acgactacgt tctttccatt ccgtccacgg
    25621 aacaccgcta ttccaggtgg ggcgagtgca ggactgatac agaacaggct ctgcttccag
    25681 cgatgccagc acgatacgcg ccagttcttc cgcttcttct gctggcagta caacgttgct
    25741 atcaggtccg tatgtttcgc gccactgctt gattgtcagc agtcgctctt tggtaatagt
    25801 gatcatgccg cgtttccttc tttcttatta acaattacac cgtcatatat ttcattaagg
    25861 tgccctctca actccatgcg ccttaatgca gataacatgt aatcgcattc aacctgctta
    25921 ttcccaataa aaggtttatc ttcagggtta ccccaacagc aattaccctt gggccaccca
    25981 tgtactttcc gtactcttcc gttaacaacg tgaagtaatc cccagccagg tggtaaatcc
    26041 tcaattgaaa taattcccgg ctcactaata aagaatcgcc agtcgcccat tccaagagac
    26101 ggatttttac gaaaacgctt ttttctatct gccaacaagt cagcacgaga acatttcgcc
    26161 tctatcaggc atgatgctga atttctgaat cccatagcat ctggctgttc tccggtactg
    26221 gttacagcta taaagcggtc atgaaaacaa accttgaacc cgttgcgctt aaggaacttg
    26281 tacgcaatct gacagagttc gcggtgtgtt aacgccatat cactctcctt tgatgcgaat
    26341 gccagcggcg cgggaatcat tccatcgctt tacttcttca cgaattacgt caatgcattc
    26401 tttcgaatcc attaggtaat cttcatcaaa aagcctttcc tgttcgtttt ctatcgcaac
    26461 aatgattgct tcaactaact tttgtgcctg agaaccactt tctaactctg caatgcgctt
    26521 ctctgcggcc tctgccctgc attgttcccg ttggaacatt ttgtgggtgc ggtcgagcgc
    26581 ctcaagtctg gctgctgatg ttttctcttc tgcttcaagc tcaacgcgca acttccctac
    26641 cgttagcgca atatcctcgt tctcctggtc gcgggatttg atgtattgct ggttcctttc
    26701 ccgttcatcc agtagtgcca gcacggtagc cgggttagcc tctgctatga attcagcgtt
    26761 tgcgtaagcc tgagcatctg tttcaaccag gcagttaaca tgacattctg caattacgcc
    26821 accgggttct cctttccatt tttgacaaac aaaaactcct gttatattct catgctgatt
    26881 gcccgatgta tgccctacga tgtagcttcc tttagttgct ttctctgccg cctcacgcag
    26941 tgcctgatag tcaatcttgc tcactggctg cctcctttgc gaatctgttc cgcccattct
    27001 tcaagggatt tctccgcata ttcaccggac aggccatcaa tcgggtacgg ttcattagcc
    27061 aactcttctt tcgctgacaa aatcatgcgt gtaacgtcga aaacttcacg caaagactta
    27121 ttgataaatc cgtgattgaa cgcagcagca agacggctgg cggtatagtt aatcccctcg
    27181 ttgcgtgctt ccgcacgaat ttcagccaga aaagcatcgg tagctggagt ttcgctgtgg
    27241 tgtagggcat cgttgataat cattgcagca acaccagcct gccctgcatc cgtgaccgac
    27301 acatgctcaa gagttacggc cattgcgtgt ttcagcccgg cgttctctgc caccagcgcc
    27361 gcgagattag tctcaagctc tgaaattcgg cacgtggcat caatatttgt gtcctccagg
    27421 cacttaattt caccaagcag ctccagtgca acctttgggt tgaacgcggc aacatgacga
    27481 gcgttgttct ctgcattttt ctgtccatca aagccggtcc atttgataac gtcttcacat
    27541 cgttcatcac cgggcgtgtg caccgcatac gtaccagtac ccggcgaaat aaatgcgacc
    27601 cattcgccct gtgttgcctg ttttgctatc tcacgcagtg cccggtaatt aatttcgctc
    27661 actggctgcc tcctttgcgg agttgcgctt cgatgcacga aaaaaaagac tcccgagtat
    27721 gactgttaag ggctggtgcg aacgccgcgt taagaacggc agcatcacag ccgtcatcaa
    27781 tatagagcgc aatttttttc tccaggcgcg ctttggcttc ctgcaactgc ataccccggc
    27841 acgcacgcgg gatataatca gcaatttgag cgatagcttt ttcgttctgt ttaaacatgc
    27901 ttcacctcga taggcttgat ggtgtctaac agcagtcgac ggcgcgtatt ttctgcaaag
    27961 tggcggcgtc cggtatcttt gtggtaaaac tcatttttgc cgacgaccca catccgctct
    28021 gtttggtgca gtttttttac ctgcggcccg tctttggtga tcacggtgcc ggtatgggtt
    28081 tttacgattg tcatgccact acctcttcga acagatgaac gagacaggcc gatgctcgtc
    28141 cacctgaaaa actgacgatt tgatgcatac tcacggttta ttcctgaatg cgcttaaact
    28201 cgattaccca caccaaggga ttagcgttcc agctttcttc accatagatg gattcccaca
    28261 ggcgctggaa cgcaaccttg gccattgcga aatccccctt gggagtaagg aatgttcccg
    28321 ggtgatcagg aagcaaactc ccagcaggcg gaacgccctc agcccttgca tcgcattcgc
    28381 tgatatcgtt caaccgttca acgcgcacgt tggtaatttc caacagaatg cgtgatgccc
    28441 atcgcggcat gtgaattgat ggacgccacc caccatcaaa cttttcattc acagtgtgag
    28501 gtttccagtc ggcatcatcg ggtatcgacc ataagccgta atcaccaggt ttttgctcgc
    28561 aactggcccg ataaatcctt gctgcgttct tctcatcgcc acgacaaagg ttgtcgttcc
    28621 agtacactgc aaccatcctc attgcctaat atcgccaacg cttcacggac ccatatgcga
    28681 tcaccaacgt taccgaacgg gcataaacca tgttcaggtg catccataac gtgggtatag
    28741 acaccattgc gtttctttgt cggtttacgg ataaccaggc agttatcggc cctgacattt
    28801 tttacaggtc ggcgagtctg cgtcttcctt ccttcaagga tggcccggac catctcatcg
    28861 ttgaaaatca tgccgcgctc tttcacttcg cctttcatgc atcccccctt acccatgtgc
    28921 gacgatgccg ccaaaagtga tagagaacag ccagaaatag atcgcggcca taatgatttt
    28981 gaatgccgtg ttcatatttt cagctcctgt gattgattgg atacatgccg cgccttgcgg
    29041 catgttttta ttttcacttc ccccgcctta aaaatcaaga tttattagag caattattgt
    29101 tgatgaagaa gcgcattttc atactccctg accatgaatg taagtacgcc gtgactcctg
    29161 aacacacgcg ccacttcaat cttatcttcc agcgcgaacg caattttact tagaccaatt
    29221 ttcttcagga gatcaatctt tgctggaccg tcatttctgt catcggtggc aggacgcata
    29281 gatagcaaag gctcagcccc gtttgttacg tacttccgca gccaggctcg tgttttatcc
    29341 cttgcgatct cacagcgccc ggttacaaac cagaccgtgt aaacgttaaa taactggcgc
    29401 accatatcaa taaccggagt gatgggagta tcggtgtcac aggcgagatt aaactcgttc
    29461 cagtcctttg ttaatgcacc tttacctggt ggcggaagca aatgcagtct gtcttcagtt
    29521 gcctctgata ttgttccatc aatatcgact atgacgatat acggacgttc ctggtgtgcg
    29581 tgtttattga aaatactcaa atgccctcct cattggacga aaaaaatgct ggtgggcgca
    29641 ctccaccagc attaaaagtg acgctgtaac tatcagcgga cgtaaatagt gccgccgttc
    29701 tctttttccc atgcatcgct acgtgcatag caaacatcga gaagtcttct tgccgcagtt
    29761 tcctctaaac ccaattcgac aaccaactgc tcatgacggc gggtaaccac atcaaacagg
    29821 gtatgcagcc ctttagttgc cagatcatca atgaattccg gttcgaacgg cagctctgca
    29881 tctgccaaca taacctcttg cgcccactca actcgacgga ccagttccgg gcggcggctt
    29941 tccatctctt tacagatcaa ttcatggaag aactctaccc aaccttccgg ctggaactcg
    30001 cggaaaattg ccaacggctg gaagtttggc atcaaccatt cgttgattcg gatatcaatg
    30061 gcatagccca tgtcgcagca gaactgataa gcaaagtcca gcttagaaac gatataagga
    30121 cgctcgttat tgaactcttt aggcgatgag atcccataag ccaggaggcg cgggaagaag
    30181 gagatttgcc ctaacgtcgg atgaagtttg cttgcaggga aacggcgctc agtaatgcca
    30241 tacatttcct tcttgagcgt cgcaaatttg gcattctcat taaccagcgc ggtaacctct
    30301 gcttttttat tagcaaatgc cacgcgcgct tcgcttgcat ctttaatagt ttttttgagc
    30361 tgttggttaa ggtcggcgac ctgcttacgc agttcctgtc gctcgctttt agctttgtta
    30421 tagcgtttct caaggttaaa aggatcaagt ttcatgatct ctttatattg agattttagc
    30481 gttgaaatct gtgagttccg cagttcaacc atcgcggtca tttcattgag ttttgtttcc
    30541 agctcaatgc ttatacgttc ggcattatca gcacgctggt tggcgtcatg cgtcgcatcg
    30601 tcgatcgcgt cctgttgctg gcgtttcaaa tgttcaattt gcagctgaag ctcttcaatt
    30661 tctttaccct tcagaccgag atccaactgc atattttcag ctgcatctac cagggagtta
    30721 tggctatcag cttctgcgtt ataaacatca ataagctgtg cgtgaagcat ctccgctgac
    30781 tgaaccgcat tatcaaaaaa acgtgctgtg aggtcatcac aactaacgcg gcgttgcgcg
    30841 gcccggatgt tctggataat ggccgggata ccggcattca ggacgtcagg gatagataca
    30901 ttttcgattg attggttttg tgctgaagtg ctcatttcaa agttccgtat tagcttgtgc
    30961 ttcggtcatt tttcctaagt atgaaggtgg aaggactacg caatttgtat ccagcccctc
    31021 acccagggca gcctgtaaaa ttctggctaa ggagagtctc ttgttgcgat accgggtgat
    31081 gacatgcctg atatcgccgg acggcgtaac aaaggcgatc agccagtagt gatatttccg
    31141 tcggaatggc cacatagtgc accttatgga ttgctctaat aaaaaacgtg atgagtgtac
    31201 atcacgtttc aaaaatatgg aattattaga gcaatattat tctgattctt gctcaaaaaa
    31261 cgcgctaatg agaggaagcc aatcctctga cacttcacgc ggtcgcggtt tgccatggaa
    31321 gaagattatt cggcagtctt ttggtaatgc cccattcccc ctggagtagc gcgcacttgc
    31381 atatttcgaa ccaggttcca caacatcggc cttgtaactt acaaaccatc ccggatacag
    31441 atcctgaaat gctggtgtat catcgcccat aaccttccgt aagaacccct gatcacccca
    31501 gcattcagta gtaacacaac gagcaatcca accttccgga tcttgccaga atgcactcca
    31561 gatatgcgct tttacactat ttggtatcca cagggcaccg ctgccacgat attgtggatg
    31621 gtaaaaatcc ctaagcatgg tgaagctggt tggtggatcc tcaaggattg ggcgtatatc
    31681 accggcaata accgtgtcca aatccagata gaacagatca tcggttatat ccggtcggaa
    31741 caactcgatt ttcgcccacc agccacggca cttttgccac tggttgatca atgggacaac
    31801 tttgacgcca ggtacatgta aacgcttcag gtctgtcagg caaataattt catagccttt
    31861 tggcagttga ttaaccagcc actgcacatc ggaagcgtta tagtcaccac cagagcgaaa
    31921 aactaaagca atcttcatgc tgcaccatca cctttcactt tcatcaatgt caggtttccg
    31981 caaaatacgg caccagtgtc gatatactgc tgattccaga atgtcttcgg gcttttcacc
    32041 ggagtgtgac caaagataaa acgatctgcg cccgaaattt cgccaccaat atcatccatc
    32101 gaatcactga tacgctcgcg cgcccagaca acgttgaaaa gcggcacctc cttaccgaat
    32161 tggtattcat tatccggata gtcggcatgg gctataacga tagtttcttg cccggtgttc
    32221 aactcaatga tatagggcag ccgtcttacc aactccacca gcgccctggc taatatttcc
    32281 tgatcagtgt ccagcatgaa gaaccattgt ccgccattca ttagccagtt attcacgttg
    32341 ccatcaggac ttaacgcatc aatcatcagc cgctcatggt tgcccataac cgctctgaac
    32401 catggcatct gcaatagctc caggcattta acattttcgg tgccgcgatc gataaggtcg
    32461 ccgaccgata tcagtaaatc ctgcgcaggg tcaaaatcca cacgatggag ttcggacatc
    32521 agtctggtgt agcaaccatg cagatcacca acaacccaga cattcctgta tttggtaccg
    32581 tcgatacggt gataaattgt gggtgcaatc atgtattctt cagccattct ttaagagtca
    32641 tctgcggaat acctcccatt ttcccgcatg aaacaacgtc aatctgttca cgcgcagact
    32701 ggaataacaa aggcaggtga cttagatttt ttggcgtgcc gccggagtga acgcgtggtt
    32761 cttgtgtagc gtcaacgccc accagggcga catgtttgaa tccgatatgg aaagccaggt
    32821 tcagagcacc atatgcacta ttgccgctgg caatttcatt ctcatcttcg caaaggccga
    32881 aatgtgcgga ccagcgccac gcccaccact cgggagaatt cgtatttttt ggctccgtgc
    32941 cgcgctcagc cacacgacgg aagcacagaa cgccgtctct gacctcacgt tctttaacat
    33001 cgggtagcgc catgcaataa caaacaccac ggcgacggcg gccacgacca acgcgccgca
    33061 tattgtctgg cgatggatca agggtgaaaa aataagaagc gcggttaagc cagtcgatgg
    33121 ccccattgac cgctataatc ggcactccgc gcggcgcaac aaagtttgcg gcgcttgggc
    33181 cactgccgac gataataacg cgatcactgc ctctaaattt attcttggga aacattgaat
    33241 tgcactgctc ctacttgcat tcaaaatatg taaatctgcg tgttttttgc gggtatccag
    33301 gaactgctgt tgccattttg aaatagacac ctgcgttgga ttccgtagtg cttgagggtg
    33361 cgcgccatgc caatgaaggc cgttttgcag agaacagtca tagccgacta ataccactac
    33421 ttcagcccct gattcagcag ccagactgat agcctgcgcg ccgctattta ccccttccgc
    33481 cggtccacaa tatcgcctgt actccaacga aaatgatttc gccgccgcca ggttggctgt
    33541 cactttgcgg aatctccctc ccggtatggt ggatccgtat tgcttccacc atgacaaatc
    33601 accggcgtat aaggcataaa tgtcatcgaa catctgccag gaattgttaa ccgcgatgat
    33661 tgaacagcca gttttttcta tagcagcaca gtcctcacga gtgagtgacg gaccgctacc
    33721 gacacaaaaa acagtcctag tcgccctggg tggtatgttc attctcagct gcaaattcag
    33781 cctccaggcg agcattcatt tcagcgatta cagggtccac tacagcatct gtttcctgtt
    33841 cattacgcgg catgaccgat gccagcgact cataattaac cttggatgac acgattattc
    33901 tcccgatgtt aaagtgcact accacaaaga gcgcacatgc actaattaat ttattatttt
    33961 aagcagcatg caaccactta tcgccgttca atacatgctc aatagcctca ccctttttaa
    34021 ggcttatgta ttccaggatg gcggttatcg cttgttctgc accatacgca agaacgacgt
    34081 agtaaccttc ctctctaagc ctgcgcatcc aggcgatctg ctctttcgtc ggggctttac
    34141 catttggttc tttaagctca attcgcatac cgtgataaat accgcacgct ttatcgagac
    34201 tcatgtccgg ataacctttt ttctgccctt cagccttcat tttcccggcg gttgcttttg
    34261 aacgcttccc tccgttaggc gttgcatgca acagctcata aatttcaggg taattgcgct
    34321 cgaagtaatc aaaaatgaaa acctgttcgt agtgctcgca atttcctact cgcagatcag
    34381 gatttttggc cagtgctgca agcgccttcg catgtggcga aacttctttt accggggtaa
    34441 gtgataagaa cggatccttt ttggtttttg gcctggaccg ccccttattt cgacgctcac
    34501 taaaagcctg aaactcttcc tcagtaaagc gcaacataat cagtcaaatc ctgccggtcg
    34561 catgccatat ttacgctgtt ttgcggcctg ctcttccctg tgccattgcg cacattcagc
    34621 gtcacaataa atgcctgatt caatctattc attgcagtaa cgacacttcc ctgtaaatac
    34681 ctggctcacg acctgtgcct gctttctgat gttatcgatg gccatgtctt tgagagcttc
    34741 taactgattc atgctcagct ctgcatcatc aacacgttct gccaattttg tttcctcgtg
    34801 aagaacctac ttaagggcag aatgatacat ttcacaatta aaattgcact aataattttc
    34861 ttttattgag ttaaatattc aacaaatgac tagcggtata atcaccatca tctatttctg
    34921 gcaggctgac tatggctaca tcaatcacta caacccaaag cacccggcaa tatcctctgt
    34981 cgcggtatga cgaccgcaac atagccgatc caatactcag ggcagagctg cgcaaagagg
    35041 tgatgcttat gtgtgaatcg aacgacaaga atctgacgat ttattacgtt cttcccgatg
    35101 agcaatatcg cccggatttg ctggcttacc gtatgtgggg catagcagag ctacgctggg
    35161 ttgtgacgct cgccgccggg cttgaggatg agtctcaggg tatgactgtt ggcaaaaaat
    35221 taaaactccc acctgccacc tggatccgcg aaatgattcg ccatttccaa tacgacggcc
    35281 aggtaatagg gacattatcc attgcgtaag ggaattgaat gccaactgaa tatgctcgcg
    35341 acaaccttgg tcgctatcag accgatggat taagtgcaaa agactttaac aaggtcttcg
    35401 atcttatccg taaacagcag cgtcagaatc ggcgaaacgc gcggcgtaca ctcaccccaa
    35461 ggattatggg gatgcgtaac cgcgaacttg aggcattcct cagccttggg aaaaagaaag
    35521 atggcaccta ctttacgccc gaagatatac gcagtttcaa cacctcaagg caggctcata
    35581 aaaccaaatt caagagcacg gtacccggca ttacctatgc tcagctggtg gcgcagtcca
    35641 ccagcattga tataaaacgc gctaacaaca aagtttctga tggcacaggg atcaaagccg
    35701 cgacattcct cgggctaaaa cacaaccttg cattgatatc tgttaatgcc tcggatgagt
    35761 cggtccacca gcatcaccgt gtcagaattc gatttgagga atgggataaa gccgttgagg
    35821 aaattgctga agacggtgcg aaaaaagccc gaatcgctgc cgatctctgc aagggccggg
    35881 tatctttcga ctgtgattgt ggacgccatc aatactggta tcgttatatg gccacggctg
    35941 gtaactatgc tgtcgcgccg ccaaaagagt atgcattccc caaaatccgc aaccctgatc
    36001 tgactggtgt ggcttgcaaa catgttttgc acgctatgac acgttttcag tctcccacat
    36061 ggcacaaggc catcattatt gccctggaaa aagcagctga acaggtagcc ttcggcgatg
    36121 acaagcggaa gacaacaacc tatttcaaag gcgaactggc taaatcgctc gcgcgcaacc
    36181 ggacaacaac gacggatcag gctaaagctg cgcgtgagta tgagctgtat ctgaaatctc
    36241 aggatgcatt aggcaaaaaa ctacgcgcaa aagatagcgc cacggacaac gttcgccggt
    36301 tgttaaaaaa agctcgcacc acggcaaaca ggaagaatgc cgaactaaaa gcatcgcggg
    36361 tgagggaagc ccaggctcgc gctgaagccg acgctctcaa aaaagccctg caaacgcagg
    36421 cgaacaacct cataaagttt ttcatgagtc agggaatgga caaggccgct gccaccgcgc
    36481 aggcgcgaag cattcttgag acacaaatta acgaagcccg taaacggaaa ggataatcga
    36541 tggctggttt ctttgatgac atgtttgagg acacagaacc atcacaacaa gtgactggtg
    36601 ataacctccc ggacaccgaa tcggatccgg atattccagg cgaaggttct gaactgattg
    36661 aagaggaaga tattgatgct gaaatcgaaa ccgatggtgt taacgttggt aatattgttg
    36721 atcctgtgga ggacaatcac cttcccaatc tggatcacgg cctgcttagt gattctggtg
    36781 tgcgccaccg ttatcaaggt catgcagttt ttaataacct tgtgcggatg gactggctca
    36841 aagcaatcaa gctagaccct gactcattcg atgcagttct gtatcgcgca ataccttaca
    36901 gagacaaaaa tgcacctgaa acggcatctg aaataataga accgaaccaa cgcatatatg
    36961 actatcagga tccagaactg ataacggccc tcgactgccc ggatgagatg gacgccttct
    37021 acgcgctata cgacggcagt gataatacag ggattagcga cagtgcttta atccttcggt
    37081 tagccgccgt taatgtgcca gtgggttcta tgctcgaatg gctggaacag ctgtcagacg
    37141 gcacaaccat tcgccgcttc tggtacatcc ataaaatatt caattacggc actgccaggg
    37201 taggcagttt gttttattgc gtgccttcac gcgcctttga agggaatttc atcggtgatt
    37261 ctgaataatc aggaatggct actggccatc tttaagaaaa aaggtcttac tccaaccggt
    37321 aagctggaat ttgccactat tgatggcatt gattcggcgc tcgcacaggc tttaaacgaa
    37381 gcgttcgact cacaagttgt cagctttaat gatcgcatta accagtcgtt ccgggagttc
    37441 ctgaaacgca caccaagaga tcgcataacg ctcggcactt ttagtgatgt gaaggagtgg
    37501 ttgtcgtcat ttgaagccga tcgcgccggg cgcaaagata cagcctctgc tggcccggta
    37561 aataagctgg caatgccgct tgtgaatctg tctcgttctc ccgcgttttc aatttatgaa
    37621 ggtgaactgt gccgggataa ttacgatgaa gggcatgtca ccaatgaaaa tgatgagatt
    37681 gaagccctgg tatctactat ccctttctca ctggaatatt cgctatggat agccagtgac
    37741 gagaaggaat ctcttgggat ggttacaact gcattagcat tctggctacg aatgtatgcc
    37801 agcctcgggc aggcatcttt cactcacatt gccaatgtcg gcggttatga gataccggtt
    37861 acctgttaca tagaagggca aaaatcaatc gcatttcagg atctgaccac cggcaccgcc
    37921 gacaacaggc tgttcgcggt tggattgaac ctcaccgttg tggcggaact tcctatcctg
    37981 gcttatatgc agcaaaccac cggcaccata acggtaaaag cgaaaattct ggaggaatga
    38041 gatggccaca aagaccacca cagccccgga aactgattca aaacgcactc agctattcct
    38101 gcaatctgtt tcaattgggc agaacgaaat ccctcgcgaa atgatcgtag gatgtaccta
    38161 tgtcgaacct ggggagctat ctggtcccca gcttatgctc atggtcaggg attcaacggc
    38221 ttacgtggtc aataagctgg gggtgaaatt tgggacaata atgacagttt cacttggtga
    38281 tccggaaggt catggcggca tcctcttctc ggaagagttc tttgttctta aagcgccgcg
    38341 caaggacgat actgtactga tttacgcgtt tagtaacccg gtgcggttat taaaagttcc
    38401 gtccaccagc gcacagtatt ttgttgataa gccaccatca gccgtagttt cctctcttgc
    38461 ccctggtctg aaggtaaatg ctgactcatt cagaaaaaca tccacatacc acctaaatgt
    38521 tggagaaaaa ccgaccaagg tattgcagga gatagcccgg gataccggtt ctatgtgctg
    38581 ggcatccagg gggacgatca attttaaaag tatggaaaaa atggcaaacg ccgctccatc
    38641 gcttacttat gagtccgcca atcccaacac atccggattt acaattagtc agttcaacat
    38701 cctgaatgcc gattatgaat accagcgccg ccacaattac agaatggcca gttatgacat
    38761 gaccaaaggt gtggtttact caggtaacca ggaagacccc attaaattta cgagcaatcc
    38821 cgatcctacc gcgctggcga actacaacaa attcattctc ccccgcctcg atatgctggt
    38881 ggaaggaaat gccgcgctaa ctccgggtac gacgctgaaa attgtcgtgc ataacacggc
    38941 aggtgacgga gaactcgatg aatctatccc tgacaaaatg atagtgatgt ccgtgactca
    39001 tttcgaagac cgcttccgtt ttgtcagccg tgcacagtta ggagtggtga atgggtagtt
    39061 tgacagggaa gtatcgggct gtagtggtaa gtgtcgatga ccctaaaggt ctgatgcgta
    39121 cacaaatacg cgttgtcggc atgatggatg ggttaccaga tgcttcattg ccgtgggcag
    39181 aagctatatt gtccaatgca aacacgtttt caccatttct gcccggcgat aaagtatggg
    39241 tagaatttcc ctacaatggg gattctcgat ggccattgat aatcggttat gcacaggatg
    39301 catccggtgg cgctcccaat gtgccgcctg aagcgtcagg acaaggtgaa ggctatgtac
    39361 cgcctgaagt cgaaggtgca ccagcacaac catcaaccag cgccaaaaaa gactttattt
    39421 cgtcgcggaa cggactaatg gagatccgga cggcgggcgg agcctgggcc gttacgcact
    39481 tgaaaagtgg aacaacaatc gggttcaacg aggccgggga gttatatgcc atttctcaag
    39541 gtccggcatt catctcttcc gcaggaaatc tcgatataaa gtcaggcgcg gatgtcgccc
    39601 tgaaggcggg gggaagtatg gcgatagagg ccagcgggaa tctatccata aaagccgctc
    39661 aagtctctgt tgataaagcg tgatggagcg aggattggtt gatatcctcc acctcctgaa
    39721 gaaattatgc ccggcattaa ccgggcattt ttttcattca gccgctactg gttttaacaa
    39781 accagcatca agtagcttgc gagttaacca ctgctggcct ttacccgtta attggggcgt
    39841 cagccgtatc tggtaaccat cttcatcatc cagcaccact tctttcaccg tgaaataccc
    39901 ggcgttgatg tactgctgaa acggtacatt tttacgtccg ccggacgcaa tcaggatgcc
    39961 gttctcccgt aaccaggcaa acagcgcatt ttgcttaagt ccaacaacct ttgcaaaatt
    40021 cccaatcagg atccccttag ccactgatac ccggtcggca aaatcgactt taggggcggc
    40081 ggccaccagc tgttgttcca actgcatttt ctgttctgcc aactcggcag ccaggcgcag
    40141 tgcttcgggg agtgtttggg ggatcgctgg agaagccgtg ctagcctgct gcaattcttc
    40201 cagtttgtcg atcagcgaac ggcggaccgc ttttgactcg cgcgcggcga ctcgcagggc
    40261 ttgtttgaaa gtcatggtga taatgtctat gtttgcacca tttttgcgac ctacactttt
    40321 tgtgtaggtc tcaccttcaa gctcatctac aaccttctca ataaacttat tatttcttac
    40381 taatggttcc ccacataact tacgcgcctc attaaccatc tttaacagcg tctggctgtc
    40441 gattgtgtct ccggtgttgg ggatagcatc catgaccggt gctggtgtgg ttgctgtcaa
    40501 aggctttgta acgacaacat ctggtttgct tgctttcatt ctgtgtgcct ccttgcgtgt
    40561 ttcggctgcg acggttgcgt aattcagatg accctgttcg agcaggtatt cgcggatatc
    40621 agacaacagg atacggtgaa ccgcgttctt gtcctttctc cggtaaagtt gtttggtgat
    40681 catgaagtag ttggcaataa cgcctggtat atccctggtg ctgatacagg cagtgtgctg
    40741 ttcaattgcc tcgatcatct cttcacgggt gactaatgac gttctcatag cccctcctga
    40801 acagaagcgt taacagggag gcaccagtaa ctgagagaat tgcgcgagtc agtagaaaag
    40861 cgtgcggaga aaatgcaggg agcatccgga agctgagaac gtgcctcatc ttctgttggt
    40921 gcgataacga agtgatagtg gcgtttctgg caggagtaaa agcgccagat aaattcttgg
    40981 cgtgcgcaag gattggcatt aaccatagtt acggcctcat gtacaggttt aacaacctgc
    41041 tacccgctgt caaacaggtg gcaggacgtg acggggttga cagactggcg tacatgaaac
    41101 cagcaggccg aagcctcccc atcacgcccc accataattc gggcgtaacg tggtttacgg
    41161 acacaaaaat accgcaatat cggatatctg cggttgtccg catgtacatt caggctgtca
    41221 aacccggtcg cagaatttac tacgacgtag gaactataag cctgaacaac aggaagatca
    41281 atagtctggc gttcgatggt agtttatttg ctctaataaa tcaaatttat tagagcaata
    41341 agcggtgtcg taaaaatcaa tacaatcgca atcatatgaa tagaatcaat cattaccgcg
    41401 cgacggtttc tgcgtgataa acgtttcaag catcttttcc gcaattgccg accaggtgtg
    41461 acactggacc ttttcagcat ttttcacgcg atcaacgcga gcaataacct catcccaatc
    41521 aatccgcgac ttgataacca tatggttcac caaagccagg cgatctggcg gaaggcaatc
    41581 tggcggcgtt aatatcaacg ccccacacat tgccgcctca agaacagtta atccaaggct
    41641 ttcgggatgc gtaacgataa aaacgtcact cttacgcaat tcagctgcaa attcggttgc
    41701 tggtaccggc gtccgtctgt atggagttac cgaaatattc cccggatcaa tggtaaccaa
    41761 tccgtcatcg gtcaacgttc tggcctcata cggaacggtc agacgctgaa ggttcataag
    41821 gatacttaag gagtgatcaa acccactaac atcaaatgca gcgtggtcta caaaaatacg
    41881 cagaacatcg tctgtcttgg tttccagatg gaacagttcc tgattcgctg cccatccaac
    41941 atgtttgtta aagcgattat gacgctctaa cctgccggga ttatccaggt accgccaggt
    42001 atcatcgcgg acagtaaaag taatatcgac tggtgccgaa tccagcatag aaccgtcata
    42061 tacctgggct acccatccag agaatcggcg acacagttgc atgcctattt ccctgggtac
    42121 cgtagtaaaa tacctcaatc ctggtgccaa aatggccttc gcagaacatg cggtcgcagc
    42181 agtcaacaca gcttcaacat aatcctccgg gctttcgacg ccaggggaat atggacgatg
    42241 gtattgcaat gttacccctg cctcactaaa ggcgcaggcc aggttgtaag cccacatttc
    42301 cgtatatgtt ttcacatcac tgatagcttc aaattttctc ccaatgatca ggatgttcat
    42361 cggcttttcc tcattccatt gcattaataa tcctcttgcc agtcagcacc agcatagtta
    42421 tcaaaccgtg agtattggcc gttaaaagcc aatctcaccg tgccaattgg gccatttcgt
    42481 tgctttccga taattacctc ggcaatgccc ttcatttcgc tatccgggtg ataaacttcg
    42541 tcgcgataca gaaacatgat caggtctgcg tcctgctcaa ttgctcctga ttcacgtaaa
    42601 tctgaattta ccggtcgttt gtccgcacgc tgttcaagcg atcgattaag ttgtgacaat
    42661 gccaccaccg gtacttgtaa ttccttcgcc aacgccttca gtgagcgaga aatctcggca
    42721 atttccagcg ttcggttatc ttgcagctcg gggacgcgca taagttgcag gtagtcgatc
    42781 ataatcatgc tcaaaccacc attttcttta taaacacgac gagcgcggga acgtagctct
    42841 gtcggcgtca gggcgcttga gtcatcaata aaaatattct gcttgtccat cagaataccc
    42901 attgcgccag aaacccgcgc ccaatcatcg tcgttaagtt gccccgttct aatacgagtc
    42961 tgatcaacgc gtgcaagaga agccagtgag cgcatcatca gctggtggct cggcatctca
    43021 aggctaaaaa ccaatacggg cttatcgtta cgaactgcgg cattttcgac gagattcatc
    43081 gcaaacgtgg tcttccccat agatgggcgg gcggcgacaa tgatgagatc ggacggctga
    43141 agccccgccg tcttcttatt gagatcggta aatcccgtat caagccccgt tacaccatca
    43201 tgcggtcgct gaaacaactc ttctatgcga gataccgttg catcgagaat gctggcgata
    43261 tcttttggac cactaccgct cttttgtcgt ttttcagcta tttcaaaaac gcggcgctcg
    43321 gccatatcca gcaattcatt gctgcccctg ccatcctgcg catatccagc ttcggctatt
    43381 tcatttgcga cggaaatcat ttcacgaaca accgcgcgtt cacgaacgat atccgcataa
    43441 gcacaaatat ttgccgcgct gggcgtgttc tttgacatct ccgcaaggta cgcaaaacca
    43501 ccggcgcgtt ctaatttacc gttctgttca agtgcttcag caagtgttat caaatcaatc
    43561 ggtttgccat gacttaataa cctctccatc tcactgaaaa tttcacgatg agcactggta
    43621 taaaaatcat cagtaactat acgatctgca acttcatccc agcggcagtt atcaagcatt
    43681 aagccaccaa gtacagcttg ttctgcacta agggaatttg gcatggattc aagaggggat
    43741 gcagacatta gcactccacc caggcgtgct gaatgtcaga tataatcggc atactcaaat
    43801 cactcctaac gatatgagtc atcaccagaa aatcaggatt aattcgccgg actcttcccg
    43861 gctgtcacac cgaatcgcca ggatggtgaa tccgcagtcc gacgctatga acggggcttg
    43921 cacattccgg ctacctggtt tgttgcctga gctaggggaa aggttacccc tttaacgtca
    43981 ccagaccgct aacgacgcat gtgccagacg ccgtgttaca accaaatatg gtggccccta
    44041 ccggacttga accggtgacc gtgcgattat gagtcgccag ctctaaccac tgagctaaag
    44101 ggccggatta ctgccaattt tgcttacgct tttatttcac cggaacaaac ggaacagcgg
    44161 tattactggt catatactgc ggtaatgtac cgttccattt gttgatcgct tccaactcca
    44221 taacaccggg gttctggcgc agagcttcac cacgtaaacg aatggcatca gcttcggcct
    44281 gggcttttgt gcgaatagca tcagcctgtc cggcagcttc cgcgcgcagc atgttggcct
    44341 ctgcttcacg ttgtttgacc tcttgctcgc gttgcagggt tttttggttt gccgtgactt
    44401 tggcgttaat actgtcaata actgttggcg ggtattctgg cttacctaca taagagaggc
    44461 tcatcacctg aatgccgatt ggcgtcattt cttcctgaat gtctttaagg gctgcatcaa
    44521 gcaattcgga tttgccaccg tcgataaatt tgtcggttgt cattttgctg gctaaccggt
    44581 tcagagcatc tgcaaccttc tggcgtagat cggtatcagt aatatcatct acacctttgc
    44641 gataggtctg aaataccgtt gtgacttttg ctggatcaac cttgtaggct acgccgatgt
    44701 ggtaaccaat ggttgttccg tcgctcatct ggaagctgaa cggctcatcg tatgtcttca
    44761 tttgcttaaa ggtcgggaag atataaactt cagtattcaa gcctgtccag tagcgaccaa
    44821 cgccaactac ttcaccgata cctttatcat cccccagctt atttactttg atccctacgt
    44881 tacctggctc tacccgatcg catccggtca gacataaaga acccaaaata atcgctgcac
    44941 taatcaacgt ttttttcatt aattaatttc ctggtttttt cacgaaaaaa gactactgcg
    45001 aaagctgggt aaatgagcgc gagaaggact cccaacaata caagtattgt gctgttagat
    45061 gagatcatat ttggcaaaag ccaaacatac agaaccagtg acacaatcaa acagaggacg
    45121 gcataaatat ataaccgcac ccatagcgtt cgacatttgt tcggattgtt ctgcatcctc
    45181 tcactccatt atttaacgaa taaaaaagct gcggtgccgg gtgcctcccg gtgtcctttg
    45241 gctggttatc caccgtggac ggggaaacaa ggagaaatga atgtactgat atagctattt
    45301 ccccgcgtgc gcttagccgc attcaccgca acggaaagag cattcttggt ggacctgtag
    45361 attgggatat gaacccgtta caggagaatg ctcttacctg ttacgtgctc cgtttcgtgg
    45421 agctaacggc gggtgatcgg gccgcaccag actggactta tttcagcgtt atgctcatgc
    45481 cagagaatca aactgtgatg gtcggtgctg aactccgaca cagggttgta gcaagccccg
    45541 caaagcgcgc actactgtag ttgcggcaca tcagcctgtg cattcaccac aatgttgaga
    45601 acactggttg tcacgctgca acgcaacatt tattcataga ttgggatatg accccgttac
    45661 gccagtgttc tcaacgttgt agtgccggtt acggttccgg ccaggcctct tcctcaacgg
    45721 ggtgttctcc atacggacta ccgtttattg gtcgttcctg cggtttatgt tgtgaagcca
    45781 gacgcttatc ttctggttgc ttcaaagagc tgcacttcat cacaacggta agggtacttc
    45841 gtagggattc gaaccctctg ccaagcacgg cgatctccga cgtcgcaaaa tacccttacc
    45901 tgttgtgttg gtgccggtta acggactcga accgctgaca tcctgcttac aaggcaggca
    45961 ctctaccaac tgagctaaac cggcaatttg gtggggagtg atggagtcga accacccgag
    46021 tcgcaatgac agtagattta cagtctcccc cgctacctct acggactaac tcccctaaat
    46081 tggcgatggt gggtggattc gaaccaccga ccagttggtt aacagccaac tgctctaccg
    46141 ctgagctaca ccatcacttg ccgggtacgt ctccggcgag ggcttccacc tccgtatgct
    46201 tttcggcgca ccgcgccctg gctgcaattc ggtaacaggg gatgcataac cctgacttcc
    46261 agcgtgatta gcgctttcag catgacggga tatacccgta aattcgtgga actgtaccca
    46321 aagtgctgtt aagcaccgct gttacgctga aaagaaaacg caacaggaaa ggacgctgac
    46381 caacagatgg ccccttctcg ttcatctggt taatcacacc agcgccctta cctgttgtgc
    46441 ctccccgttc cctaatacac agacggggac actctgcggt cgattttttg acgggggacg
    46501 actcataccc cgtggcatct ggcttcttag gccgctacca tcatcagatc atcgtttgca
    46561 tttactttaa tggtcagttt ctaaaccgcc gcaaagtcgc taaccatgac gaaaaccctg
    46621 aaaaaaacgc ccacccgaag atgggcaaac tggaagctcg taacgcactt cggtgttgcc
    46681 acttaggcgt atggtcaacc tggcaactcg gcgtcatgat ggggaaggag tcactacccc
    46741 gccatacttg ccgccgcgcc tgtcgcggct aacagctaaa tcgctctata aatcacgatt
    46801 cattgagtcg atattacact aataaattta ttagagcaat atagctataa cgtcatgagc
    46861 aacacctcga gtgtctccct tacaagacac agaacgtctg gcaaaaagag gttccactct
    46921 gaagccactg tcatgataaa gctctctgat gtttggcgcg ccactgttag taatgagaac
    46981 tttcgcacct cgacgatgag catccgtcaa cagagacacc aggcgttttt gctcttcaaa
    47041 cttaaagtca tgaccggaat agttcgtgaa tccctctgta tttggaagcg gttcatacgg
    47101 cggatcgcaa aagatgacat ctccttctcc ggcagcttca atcaccgctg caaaatcacc
    47161 gcatacaaac tcagaccgcc cttccgcacc gaggaaggct tccatctcct gtaatgggaa
    47221 atacggagtt ttatacttcc cataaccgac attgaactca ccggcctggt tgtaacgcgt
    47281 caatccgtta aaacaatgtc ggttcaggaa caaaaacgcc gctgcgcgat gtaaatcatc
    47341 atagacttgt ttgttaaacg cattccgtac tgccaggtat ccttcctgtg tgttgtagtc
    47401 ctggaagaaa cgatgtgcca gagtgataag tgaatgcgcc tcgcgttgca gagtcttgta
    47461 aaagttaatc aggtcagcat tcacatcatt tagcagattt tcctggtatc cggcattcat
    47521 gaagacagct ccgccaccaa cgaaaggttc aatcaggcgc ttcccttctg gcaaatagcg
    47581 aaagatttgt tccagaacac caaattttcc accagcccat ttgaatatgg accgttcgaa
    47641 ttctgccgct ggtttaactt ttcgctcttt tgtttcactc ccttccttct gccgacatgc
    47701 agccttggca atccgatcgc caatccagcg cattactggt atcgccatac tattgccgat
    47761 cgctttgtaa cgcggtccgt cagctgcaag catcgcggcc tcttcttcgc ttaaatctgg
    47821 atagtgattg cgaagatatg ccagttcatc tgaattaact tttttacgct tttccgtcgg
    47881 gatcaacgta tgcccatcag gaaaaccttg cagcctttca cattcgacag gggtaagacg
    47941 gcggacagct acttctgcgt ttcttacttc atagcaaaca gctgttggat ttttagccat
    48001 tagagatggt gaagtattct tagttgcagc atgttgtgta ccgctcatac gctcaggaaa
    48061 agccaatgta acaagatgct catggctttc ttgctcacgt gcccgcaatg taccatgccc
    48121 ttctgaccaa aaacctgctc ctgtgctgct aaaaacggca aggtcagtgg catctttaaa
    48181 atctcttgcc tttactgtcg atgcggtttc atcgtcaata tattccccaa atgctgccat
    48241 cctgaaagcg tttacggctt tcgtcgattt catactgggt ggcatgtcag cgtgtaggca
    48301 tggatttagg ctttcgccac tgattgcagc gccatttgca ataatggcgg aagcgatttc
    48361 cttctttttt cggctcggcg caatattccg gcgcacgcct tcgaactcaa aaagtaccgt
    48421 tgcgggatcg agatctgttc gagcacttgc gacaacaaac acgcgtcggc gtcgttgtgc
    48481 cactccgaag tattgggcat caaggattct ccaggccact tttcgctgcg gtccataaat
    48541 acaaccacac tgcggccact ttggaacatg gcaaccggtt ttgccatccc accgccagaa
    48601 cgcgttgctt tttcctgatt caggtcgatc acctggttca aatggcacat cttctccagc
    48661 caatccggca aggaaacatc cgaaggcgtt atctgccgat gacaagactc ctgggacatt
    48721 ttcccagacg ataactgtcg gtttgaggaa ggactcagac cgtttgtcgt caattgcatt
    48781 tgcaagctcc acatacttca aagttagcgc gccgcgttca tcatcaagcc caccacgtaa
    48841 gcccacgata ctgaatgcct gacagttatg gaccaccgca ccattgagga tataggaatg
    48901 atcaccttcg acttctatgt tgtatacagt atctaggcct accgatttaa actctttgac
    48961 tgttcgtaaa agcattccat gagccaatct tgatttacgt gacaatttct gcgggcagat
    49021 tgttacctga taataattcc gttgatttac cacgcgatcc tcgatcactt ttttgggctc
    49081 aacttcaata aagctgaccg aagaaacata accacaagtc tgtgacaacc ccgcaacgcc
    49141 ccaagcaagc gcagaactaa cactattaat tctaaatccc gctttaccac ttggtgtccc
    49201 atcagtatca agatagcctt gtaaaaacac atgacgcaat ggatgcgaca tcacccatgc
    49261 agggatacgc ttagcatggc ttaactcgcc aaaatgttca ttaagccaat tggcataaca
    49321 cgtatcattc aaggttactt tgatgctccc tcggatttcg cttgccacgg aaaatatgtt
    49381 ttctggtatg cggcaatgaa actttctcaa tttctggcaa tttatgccaa aaacaaccgc
    49441 cttcttagat ttacctctcc atctcctaat atatccatcg ccaacataag cgcccgcaag
    49501 atacatagcc tgttcttcag acaagaaccg agaacaaata tctggaaatg caatattgaa
    49561 attagttaga gcgcaccatt gatatcccgg catatcacat gctgctcgcc attccggttc
    49621 agacaacaac tctctcttaa aatatgtgcc atttttccgg gtgttttggg ctttccaccg
    49681 aacagccagg aagggatggt cattggttgt tcttatacct aaaggctgcc caacggcatt
    49741 aagtaacccc gtattagcta tttttgaacc aactcttttt acttgttgta accgcccgag
    49801 atgactgact acgtaatcac cagggcaaac attttctatt ggtttatagc catttttaca
    49861 aagaaccata tgccccgcgg taaaacatgg tgttcccccg acgagcacgt caggggattc
    49921 gatttcccca gccaagactt ttttggcaag ttttttcatg tcgccaaggt tggcaacatg
    49981 gggccagcgg agcgcaagaa cggccgatgg aaaaggctcg atttcagcaa accacgccgg
    50041 acgcataccc aacggttccc aggcaatact cgcggcttca attccactgc aaacagatcc
    50101 atagcacagc tctttcactg cttagcctct ccgccaaggg catttaccag agcatcaacc
    50161 aggcacgaaa tttcactggt caacaggaag aaatctgcgt ccagtcgctg cgcaacatct
    50221 tcactatcaa tatcagagtt ctgctcaagc aatccatccg caaatttgac gctggtaagg
    50281 ctgaagttat ggtccagtgt aaatttaatg cggttctgcc agtcgagtgc caacttagtg
    50341 acgagcttgc cagcttccag gtgtgtggaa atttcatcgc ttcccaaatc ctgctttttc
    50401 actcgggcaa taccgccatc ctcaagcact gccttaagtt ctgccgcatc ccccatttga
    50461 aatccctgtg gagcactacc atcacgtacc cagtcggtca gcgttaattc aatgggattt
    50521 tcaacactca ggggaacagc aggaagagaa cccagagacc tacgcataag cgcgagcata
    50581 tcctctgcct gccgcgcgct ggcattgata tagatacgtt tagttgaacc gtcgtagatc
    50641 gcctggataa cagaaaactt tgaaaaagcc cgtggcagaa gagaatgcag aacttcgtct
    50701 ttcagggagt ccttctctgt tttcttcagt ttacgcgctt gttcttgctc aagtttttca
    50761 attttttctt gaatagctcg ctggataacc ggcgggggaa gaatttttgt ttcgcgcttt
    50821 gcttcaacaa ggataaaacc atttccatgc atagcgataa cttcagaatt atcaccaaat
    50881 ggcgatacaa aaccgaactt ggccatatcc tgactaccgc atggcgtgaa aaggatcatt
    50941 ttctttttat cttctaagtc ggtcagatcc gcctcacgag aaagtttata aatagtaatg
    51001 tttttccagt gcttaaacat gttgtaaccc ttgaatatca accacagaaa gctcgtcttt
    51061 gtagaaaaag gccaggttgt ggcaccccct cgtttgagcg tatgagctgg gaccaatttc
    51121 gttcttccag acaaatggct tcaaatccgt acggcgaagc ataaaaacgc gatttattcc
    51181 gctctgattc ccaatgaggc aaaagccttc tttcaccttg atagcctgca agttgtcgag
    51241 ttcaccgctg gttacacggc tatcgaactc tttgcggctt attagctcca tctgcatctg
    51301 acgactccaa acaaatgccc attgaagggc gatggctgaa tggtaccgaa aatacgacat
    51361 aaaaaacaat atttattaga gcaattttgc aataagtaaa cgccatacag accacaaata
    51421 acctaagtta aaataacgaa aatcagagca aataattggt gatggcgtgg caagtattgc
    51481 aacaaaagac agcatttgtt cggggcacgg aggattccca tccaggcctc ctgtagagag
    51541 tgaaccacta cttaaagtca acggagtcga agtgttagtt gatggtaagc aatatgcaca
    51601 gcataccgat ggaaacagta cgcacggtgg gcaagctata tcaaccaggg catggtttac
    51661 cgtcaatggt aaagggatcg tatgcgttgg tgaccctgtt tcatgcggat ctaccgtagc
    51721 gtccggagac ggcctggttc aggtaagtta ggagatatca tgctggaaaa agactaccag
    51781 ttatccgcat ataaaaaatt ggccgccgcc ggtgggatga aaacacctgg tgccataaca
    51841 tcggcacgaa acagtgctaa cacagcaaaa ctgcttgcag aagaattgac cggattaatt
    51901 ctggatacaa ttgtctatcc cgacactatt accagctatg tttcaacgat cagaacaacc
    51961 acaaccggct taacgaacat tggagaactg gcaactaagc acgcggacct gttggctggt
    52021 tatgcagatc tgtcaatgct gcttcaactc gatattggtt gggatgttta ctgccgtgct
    52081 aatgagcgag aagtttcaga actgccgatc tctattgcca ttggtgatgt gaatattact
    52141 aaatcgcttg aggacgctgt taacgcgctt aatacatcaa gtttagtcgc tgcaatgggg
    52201 gagattaacc aggcccttaa cactggctca gaaagctcgt caggctctgg ttcaggcggc
    52261 ggcactgcca ctcccccacc agcactaaca gaagagcaaa ttgaatctct gaaagtagca
    52321 actgaacagt ttggggttgt tttcaaccag acaacagcac ccacaactgc gttacaacag
    52381 cagtatgaac gagcgaatga aagcgccaac gtagccataa ctgcttataa ccatgctatc
    52441 ggtaccgcgc ttgcggaagc atcagcaaat aaggccagca cagccagcgc agtcgccgct
    52501 ttggttcctg attctgttct tgatgaatta aacaaagcgg cacggtaaca aaggacttca
    52561 ttgataattt ttcttcagga ggaagacatg tcattctttt ctacgttaaa aacagctttg
    52621 tctttgaagg agaaacttgc tgctactggt gttcttgttc tgatttgcgc acttgttggt
    52681 gctgggtttg catgggaacg tcatcagcta aagcaagcca tggagaaaat tggcagtctt
    52741 gatcaggctg ttaaggaacg tgataagtca ataatggatc ttaaccagac cattgagacg
    52801 atgaacaaag cagagcaaca ttttcacagc caggaagtga aaaatgaatc agaacaagcc
    52861 aaatatgctg acaggcaaat ggaacgaaaa gctgaagttc agaaacaact ggttgcggcg
    52921 ggtaatgttc gccagcgcat tcctgctgac actcagcggt tgctccggga gtcgatcagc
    52981 gaatttaacg ccgacgccga caaaggttaa ccaccctgcc cccaaaagtg catttatgtg
    53041 cagaatgcca gagtttagca gtgaatattt tgatgatctg ccagcgtata tcctcgatac
    53101 agaaacgatg ctgatgggga ttaacaggaa gaatcgcaac gttaatgatt acaaccgcgc
    53161 tatcagcggt aactaaaagg gatttttatg tctgataaag taacagtaaa gcaaactatc
    53221 aacaaagcga cttcaatcta caaaattgag cacatcactg ttggcaagcc aggatctgaa
    53281 caataccgtc atgctttcga gcttgccgat cagcttggtt taaaacaccc ggattgcatt
    53341 gagcatgtat ttccgaccta tgctgatgag caatgtactc atgttcttac cgaagaggat
    53401 tttttcagca ctgaagaacg agaaggcgtt gatcgctgca ttggtgtgat ttgctcttca
    53461 gtgagttatg agttattccc taatgtccat gaaaatggtg gtattggata ccaattcctg
    53521 tacgaaggcg atgagcttaa atgttatgaa catggtcttc ttatcgaaag cgtagaataa
    53581 tacccttcct tccaaccggc tatgttggcc ggtttattca acttatccac agcatagatc
    53641 caataaacag atcctaaaga gaacccagga agatccaaag aagatcccgg atcgctgtaa
    53701 gccgcgccat ttctggcctg aaatgggatc aacattgact atacgcgatt ttatgttgac
    53761 tgtgcacgat ttattgttga ctgcacgcga tttattgttg actatatgcg acagaaacat
    53821 tgactgtacg cgattttaga gcctgactat tcacagttgt tgataactgc aatccagatg
    53881 acgccaggcc gcgccacata tggagaaacc acgatgctgg aagaaaataa aggcttcctt
    53941 agcgttgaag aagttgcagg aaatacagga gaaatccaca gcctgaaacc caataacaat
    54001 agcactatac aacccattgc tttgttgcgc ttaggtgtgt ttgtgccaac cttaaaatct
    54061 accaatgtgg cactacgtcg cggatcgtca gttactacaa acacaacgaa cgcaaccgaa
    54121 gaactatcaa gcctcaaaat tgttgagcag gaaggctatg agggaattga aattcatggt
    54181 ccacgcctgg atatggatac tgattttaag gtgtgggtgg gcataacctc cgcgttgttt
    54241 gactacgcac ctgatgatga cggcataatc accctgccat tctccgagtt tgccgatcga
    54301 tgcggctatc cacgtaagcg cctttcaaag gcgttccgta aaagtattga tgactctctg
    54361 acacgcattc agcagacagt tgtcaaattc cgcttcccgg cggcaaaagg tcatctcaat
    54421 aacattaacg tcaacttgtt ggcatatagc agcctgaata ccgagcttga tgttatcgag
    54481 atccagccgc agaaacagct atctgaactt tactatgttg actataagcg aatcctgaag
    54541 ctgaagatgc tggataagct cgggcgcaaa gagacggcca aggtactgta tacattcttt
    54601 gaggctctac ccgccaaccc ggcacctgtc agcattgagc gccttagagc aaggcttaat
    54661 ctcaaatcat ccgttagcgt gcaaaatagc gttatcagaa aagccatgaa agatttggaa
    54721 gctattgaat atcttaaatt ttcagagata aaaaacggca ggaaaatcgg ctttcagatc
    54781 cataagcgca atccataata ttgactatat gcgatagcga gaagttgact ataggcgaca
    54841 ttcgttgacg ctggtggatt tttgctggcg tcaatattct gcaagtcgct attgagatgg
    54901 cttttagggt catttcatcg cgtatagtca acgtttctcc cgacaatatc ttacatagtc
    54961 gatctttggt ggagttaaat cgactacagt caacttttga ctgtagtcac atcgcgcata
    55021 gtcaactatt cacattaacc ttcgcgcata gtcaacattt gcgcggttct catcaagcag
    55081 tggtattgat atgcaagaag agaaacaaca ctacctctac gttctggtgc cggagaacgg
    55141 agatactttt aaaatcggca tttcatgtgg tccattggca cggtttaaag ggctacaagt
    55201 gagtcccgat tttgcgcttt cacgggtcta tcgtggtacg cgtttggcaa tggttaatct
    55261 tgagcgggct ttacacgcaa cctttttccc ctggaatgcg ccgtgggaga aaagcgccgg
    55321 tggcgggcat actgaatggt ttacacgaga gtgtcttgat aaggttttgg ctcatatcga
    55381 atatctaaat gatatgtggg gagggattct cgagcgcatt aagtcgaatg atttacttca
    55441 gcgtccagta gatgctgctc gctctttcga aaaagaactg gatgttactt ctatcgtgac
    55501 tttcaaagat gacgcaggaa tgagggacgt ggcttatgtc tccatatctg gctatgaacc
    55561 ggacgcgatc cgcgctcaat gtgaattgct gaaagcaatg tttatgcttc ggactaaata
    55621 tccctgggag acagggcggg tgtgcttccc tatggaagag ttaaccgcca ccattgattc
    55681 tcagctttac cacgataatc cagagaagtt ttttagcctt ttagccggta atgggcttaa
    55741 ctgtgtgtcc gggctggggc gaagtagaat ccaacatgcc tcgctcttcg gtcccttctt
    55801 ttacgatcga cacgggtact ttgaggctga acttccggcg cttacgcgtg ctatagatac
    55861 tatcgatttc gaacgattat tcgctgctct tagcaaataa cactgatgcc cctgaacggg
    55921 gcttttttgt gcctttcttg taactctcaa tcgtgcaaaa tgaacaaaac atgcagagaa
    55981 tgccatgtac aagcatctac gcatacatta ttattttatg cagcattttt aattaaattc
    56041 aaaaatacag cataaaggat gactttcgat gagtgattcc agccagcttc acaaggttgc
    56101 tcaaagagca aacagaatgc tcaatgttct gactgaacaa gtacagttgc aaaaggatga
    56161 gctacacgcg aacgagtttt accaggtcta tgcgaaagcg gcactggcaa aattgcctct
    56221 actgactcga gcgaacgttg actatgccgt aagtgaaatg gaagaaaagg gttatgtttt
    56281 cgataaacgc cctgctggct cttcaatgaa atatgcgatg tcaattcaga acatcattga
    56341 catatatgaa catcgcggag tgccaaaata ccgggatcgc tacagcgaag cgtatgtgat
    56401 tttcatctcc aatcttaaag gcggtgtgtc aaaaactgta tcgacggttt ctctggcgca
    56461 tgcaatgcgt gctcaccctc atcttctgat ggaagattta aggattctgg ttattgacct
    56521 tgatccgcaa tcttcagcaa cgatgttttt aagccataaa cactctattg gtatcgtaaa
    56581 cgcaacatct gcacaggcta tgttgcagaa tgtaagccgt gaagagctgt tagaggagtt
    56641 tattgttcct tctgttgtac ctggggttga cgttatgcct gcgtcgattg acgatgcctt
    56701 tattgcatcc gattggagag agctgtgcaa tgagcatcta ccgggtcaga acatccatgc
    56761 tgtcctgaaa gaaaatgtga ttgataagct gaagagcgat tatgacttta tcctcgttga
    56821 tagtggtcct caccttgacg ccttcctgaa aaatgctttg gcctcggcca atatactgtt
    56881 tacacctctg ccgccagcaa ctgtcgattt ccactcatcg cttaaatacg ttgcccgcct
    56941 tcctgagttg gtgaaactca tttcggatga aggctgcgag tgccagcttg cgactaacat
    57001 tggttttatg tccaagttga gtaacaaggc agatcataag tattgccata gcctggctaa
    57061 agaagtgttc ggtggggata tgctcgatgt cgtcctccct cgccttgacg gttttgaacg
    57121 ttgcggcgag tcttttgaca ctgttatttc agctaacccg gcaacgtatg ttggtagtgc
    57181 tgatgcattg aagaacgcgc gaattgccgc ggaagatttt gctaaagcag tttttgaccg
    57241 tattgaattt atcagatcta actgaggagt aagaaacccc catgtcaaag aaaaacagac
    57301 caacaattgg gcgaaccctt aatccttcaa tattaagcgg atttgatagt tcttcagcct
    57361 ctggcgatcg agtcgagcag gtattcaagt tatcaactgg tcgccaggcc acatttattg
    57421 aagaggtaat acctccgaac caggtagaaa gcgatacctt tgttgatcag cataacaacg
    57481 ggcgtgacca ggcatctctt acgccaaaat cattaaaaag tatccgaagc actattaagc
    57541 atcagcaatt ttaccctgca ataggtgtta gacggggtac agggaaaatt gaaattttgg
    57601 atggttcccg gcgtcgagct tctgccatct tagagaacgt agggttgcgg gttttagtca
    57661 cggaccagga gatcagcgtt caggaagcgc aaaatttagc gaaagacgtt cagacagcat
    57721 tgcagcacag cattcgagaa ataggtctgc gtttgatgcg aatgaaaaat gatgggatga
    57781 gtcagaagga tattgcagcc aaagaagggc tgtctcaggc taaggtcacg cgtgctctcc
    57841 aggcagcgag tgctccggaa gaattagtcg cccttttccc tgtgcagtcg gaattaacct
    57901 tttcggacta caaaacgctt tgtgctgttg gcgacgaaat ggggaacaag aatttagagt
    57961 ttgatcagct tattcaaaac atatccccgg aaataaacga catcttatcc attgaagaaa
    58021 tggccgaaga tgaagttaaa aataaaatcc tgcgcttgat aacaaaggaa gcctcactac
    58081 tcacggataa aggttctaaa gataagtccg tagttactga attatggaaa tttgaggaca
    58141 aggatcgctt tgcaaggaag cgcgtgaaag gccgtgcatt ttcttatgag tttaatcgac
    58201 tttcaaaaga gctacaggaa gaactcgaca ggatgattgg gcatatcctt agaaagagcc
    58261 tcgataaaaa gccgaagcct taaactttcg ccattcaaat ttcactatta acctactgtt
    58321 tttaaagtaa atccatctaa aatttcaagg tgaaatcgcc acgatttcac cttggatttt
    58381 accttcctcc cctactcccg aaaaaataaa aaaattgctt gtcacgagaa agtcaacaag
    58441 tgactttcaa taaaatctct tccgaaaagg gattcacaca agtgccttgt gtttaaggaa
    58501 gagtaaattg agtaacttac gcgaatacca gaatcgtatt gcagatatcg caaaacgctc
    58561 taaagctgtg cttggctggg caagcactgc gcagttcggt actgataacc aattcattaa
    58621 agatgatgcc gcgcgtgccg catctatcct tgaagctgca cgtaaagacc cggtttttgc
    58681 gggtatctct gataatgcca ccgctcaaat cgctacagcg tgggcaagtg cactggctga
    58741 ctacgccgca gcacataaat ctatgccgcg tccggaaatt ctggcctcct gccaccagac
    58801 gctggaaaac tgcctgattg agtccacccg caatagcatg gatgccacta ataaagcgat
    58861 gctggaatcc gtcgcagcag agatgatgag cgtttctgac ggtgttatgc gtctgccttt
    58921 attcctcgcg atgatcctgc ctgttcagtt gggggcagct accgctgatg cgtgtacctt
    58981 cattccggtt acgcgtgacc agtccgacat ctatgaagtc tttaacgtgg caggttcctc
    59041 ttttggttct tatgctgctg gtgatgttct ggacatgcaa tccgttggtg tgtacagcca
    59101 gttacgtcgc cgctatgtgc tggtggcaag ctccgatggc accagcaaaa ccgcaacctt
    59161 caagatggaa gacttcgaag gccagaatgt accaatccga aaaggtcgca ctaacatcta
    59221 cgttaaccgt attaagtctg ttgttgataa cggttccggc agcctacttc actcgtttac
    59281 taatgctgct ggtgagcaaa tcactgttac ctgctctctg aactacaaca ttggtcagat
    59341 tgccctgtcg ttctccaaag cgccggataa aggcactgag atcgcaattg agacggaaat
    59401 caatattgaa gccgcacctg agctgatccc gctgatcaac cacgaaatga agaaatacac
    59461 cctgttccca agtcagttcg ttatcgcggc tgagcacacg gtacaggcgg cgtatgaagc
    59521 acagcgtgaa tttggtctgg acctgggttc cctacagttc cgcaccctga aggaatacct
    59581 gtctcatgaa caggatatgc tgcgtcttcg catcatgatc tggcgtactc ttgcgaccga
    59641 cacctttgac atcgctctgc cggttaacca gtcctttaat gtatgggcaa ccatcattcg
    59701 tggcaaattc cagactgtat atcgcgacat tattgagcgc gttaaatctt ctggtgcgat
    59761 ggggatgttt gctggtgctg atgcagcatc tttcttcaaa cagttgccga aggatttctt
    59821 ccagccagcc gaagactata tccagactcc gtatgttcac tacatcggta ccctgttcgg
    59881 taacgtgaaa gtgtacgaag tacctgctgg tatttgtaag aacttaacga cagagaacat
    59941 tcagttcagc tcgatggatg tgctgtgcta cgtccgtgat gaaaatccgg gtaaagcagg
    60001 cttcgtgact ggtgatgctg tcccggctat cccgttccag catccgacca ctccggcgct
    60061 ggtcaaccgt accacactgt ggggttcggc tatcaacgat atgcacccac gcaacggcgc
    60121 tgattacttc actcgtgtaa cgctgacaat ggccaaaaaa ggcgggctta acttcataag
    60181 cggcgacacg attgatgccg gtgactctga gtaatcaggg gaagttctcc gtttaacata
    60241 gcgcccccgt gcggggcgca taacagggaa agttatgtct caatattcaa ttcaacagtc
    60301 attaggtaat gcatccggcg tcgcggttag cccgatcaat gccgatgcga cgttatctac
    60361 cggtgttgca ttaaatagca gcttgtgggc tggtattggc gtatttgcgc gtggcaagcc
    60421 gtttactgtt cttgcggtta ctgagtccaa ttacaaagat gttctcggcg aaccgctgaa
    60481 gccgtcttcc ggctcacagt tcgaaccaat tcgccatgta tacgaagcta ttcagcaaac
    60541 gtcaggttat gttgttcgtg ctgttccgga tgatgcgaag ttcccgatta ttatgttcga
    60601 tgaatcaggc gaaccggctt acagtgcgtt gccatacggt tctgaaattg aacttgatag
    60661 tggcgaagcc tttgctatct acgttgatga tggtgatccg tgtatttcac ctacccgtga
    60721 gttaaccatc gaaacggcaa cagcggacag cgcgggtaat gaacgcttcc tcttaaaact
    60781 gacccagacg acttcgctcg gcgtggtaac gaccctggag acacacactg tgtctttggc
    60841 ggaagaagcg aaagatgaca tgggccgctt gtgttatctg cctacggctc tggaagcccg
    60901 ttctaaatat ctgcgcgcgg ttgttaatga agagctgatt tcgacggcga aagtaacaaa
    60961 taaaaaatcg ctggcgttca ctggtggtac caacggtgat cagtcgaaaa tctcaaccgc
    61021 tgcgtacctg cgtgcggtta aggtgctgaa caatgcgccg tacatgtaca ccgctgttct
    61081 cggcctgggt tgctatgaca atgcggcgat caccgcgtta ggtaatatct gttctgatcg
    61141 cctgattgat ggcttctttg atgtcaaacc gacattgacg tatacggaag cgctctctgc
    61201 tgttgaagat accggtttac ttggtaccga ttatgtaagc tgtgctgtct atcactaccc
    61261 gttctcctgc aaagacaaat ggacccaatc ccgtgtggtc tttggtctgt ctggcgtggc
    61321 gtatgcggcg aaagctcgtg gcgtcaagaa aaactctgat gtcggcggtt ggcattactc
    61381 accggctggt gaagaacgtg ccgtcattgc tcgtgcgtca attcaaccgc tgtatccgga
    61441 agataccccg gacgaagaag caatggtcaa gggccgtctc aataaagtat ctgttggcac
    61501 ctctggccag atgatcatcg acgatgcttt aacttgctgc acgcaggata actatctgca
    61561 cttccagcac gtcccatccc tgatgaatgc aatcagccgt ttctttgtcc agttagcccg
    61621 acagatgaag catagcccgg acggtattac tgcggctggc ctgactaaag ggatgaccaa
    61681 acttttagat cgctttgtcg cctccggcgc tctggtggct cctcgtgatc ctgatgctga
    61741 cggtacagaa ccgtatgtgc tgaaagttac gcaggcggaa ttcgataaat gggaagtagt
    61801 ctgggcctgc tgcccgactg gcgtagcccg tcgtatccag ggcgtaccgc tgcttattaa
    61861 gtaagggaat acaatgagca aaaacttttt tcaatccggg gcatttttgg ggaatggact
    61921 gtctcgtttc gctttgaact ctgatcctgt gcagctgatg gagtctgccc gagcaagcgc
    61981 cgaacagcca acagatccgg ttattaataa taatccggaa ccggcggcac agactaacga
    62041 taacgttcca tctgccccgg ctcctgagca aatcctggaa gggaaagacg gtaaagaatg
    62101 gaccgtcgaa caggcgcacc agatgattct ggaagctgca aatagaagtg ctatgcagaa
    62161 tgcgttgagt gatgcggccg acgccgtttt cgcctgggct gatagcggtg atctgacttt
    62221 cgactccctt gatggtttcg ttcaggctat cgctggtatc tctgatgacg acgactccga
    62281 agttacagaa gaacaggacg atgcctataa cgaagcatgg gcaaatgttg ctgacttcct
    62341 cgcagcatgc ggtgtagatg atgacctgat cgaagcactg gctgacgatg aagacgacga
    62401 cgcagctgct gatgttggtg cctctatcgc tggtttagat agcgacgacc gtgacgaact
    62461 ggaagcggcg tttgttgttg ctggcacttc tgatgaaatg ctgactgaag catttaagaa
    62521 ggttgttcgt aacggtgaga tcaaactcat ccgtaaacgc ctgcgtaaaa aacgtctgac
    62581 tgcggctcaa aaatcggcgc tgaaaaaagc gcgtcgaaaa gcccagacgg gtgcggcaaa
    62641 acttgcccgc aaaaagtcaa tgaaactgcg ccgtaagcgc cttggctaaa ggaggaggcc
    62701 ggggaactcc ggcctttaac ttgaatggct cctatagctt atggggttta cagccaggct
    62761 gacggtgtat cgccatatct gaaagttact ttaacgaact ctcagtacca ggttaccgga
    62821 tatatcagcc agggagcggc aatgaacatg gcccagaatt gggaagcgcc gtttaccggt
    62881 atgtccatgg gatctgtttc tggtgctctg ggtggttttg tgcaagtagg tactgaaaca
    62941 acgtcggttg cccgttggaa tagcttaatg gtttgggaag gggggactcc gccgacgttc
    63001 acgctgcctg taactttcat tgctttgaac aatccattca ttgaagtttc aggcgctatc
    63061 gccgcgttga cagccatgat tagcccggaa ctaaaagcgg ccaatgttgg tggtcgaatc
    63121 ccggagcgcg tgacgctaaa cattggtcgc cggatcaaca tcaccgatgt cgccatccag
    63181 gacttaagtt ttgatctcga tgcgccaagg gacagtaatg gatatttcct gaaaaacacc
    63241 gtcaacctcc agttgaccgg ttcttcgata tataacagct ccgatattgt tcgggcgttc
    63301 cagtaaaagg attttatatg gggcacaata acactaaggg aaaccgtaaa tttattaagg
    63361 gccgctatac tgccaacgcg gccaaaggcg aacgactggt atcttctgaa ttccagctca
    63421 ctgttgcagg ccatgaagat atcagcgtac tggttcgcac gtcgcaaatt cctgaaatga
    63481 cccgcgagga tgtggaggac tatggtccga atggtgtgaa gttcaaccag cacggaccaa
    63541 ttcgaaactc tggggaaatc caggtccagt gcgtggagac tatcgaaggc gatattcttc
    63601 agttcatcaa ggatcgcatt gcggcgaagg actatgttga tatcacgatg gctgctaccc
    63661 ctgaatccaa atcttccggg gttaacgctg tgacaaaagc tgctacaaca attgaaatgt
    63721 tggactgcaa aatctacagt gatgcaatcg actttagtac cgaagatgtg actgccgctg
    63781 tgcgcccgtc acttcgtatc gtttacaact ggattgagtg ggattaagag tcaccccttg
    63841 tattttaaag ctcctacggg agcttttttg tgcttaaatt atgccggtat agcgaaattt
    63901 tgagcatatt atggaaattg atttttcata ctctcctgaa acgatagaac gaagattcga
    63961 aataattgga tgcaaaacta tttcagagga gcattattgg attctctatg atgccaatac
    64021 atggttatgt gccttagcag aatgtcggcc atcgttatgt gcaggggagg gaggacttcg
    64081 acataaggtg ctggctacac ttgaagtgaa cacgttacga tattggtgtg tcgaaatact
    64141 tagggataac aaagaattac atttattatt gctgaataag tgtgcgcctc tgcgcaggaa
    64201 agcataagaa aacccaaccg ccatactgga attatcagaa gaatatgtgc ggaaagttat
    64261 agctagctat tgaaaagcta aagtagatcg gttagattta ccttactcat ctaccatgcg
    64321 tagaaagagc gatcgaaccc gatacatagc aatatgtgtc gggttcagct tttttatgtc
    64381 ccaagtgctc tttttctgct ttgtttgagt gttctgattt tactgtcata ggttacaaca
    64441 taggctgttt tgagaagcca atagtctcta cgtgcggata gcacaacaac gtagttttca
    64501 cactcgttcc agattaccca ctcttcagtt ggtcctcgtc tttcagacca acatgatata
    64561 tcgcaatggt cataattcct tatcatatgc gcgatccagc ggatccgctt acaccgttca
    64621 tcgataggaa tttaaatccc aaaaagatta aaaaaacacc c
//
LOCUS       DAZGJW010000009        62862 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZGJW010000009 DAZGJW010000000
VERSION     DAZGJW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.009"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(64..1308)
                     /gene="mtr"
                     /locus_tag="ACHKIK_000621"
     CDS             complement(64..1308)
                     /gene="mtr"
                     /locus_tag="ACHKIK_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO9081852.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(1462..3351)
                     /gene="deaD"
                     /locus_tag="ACHKIK_000622"
     CDS             complement(1462..3351)
                     /gene="deaD"
                     /locus_tag="ACHKIK_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO9081853.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEEQDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(3344..3424)
                     /gene="yrbN"
                     /locus_tag="ACHKIK_000623"
     CDS             complement(3344..3424)
                     /gene="yrbN"
                     /locus_tag="ACHKIK_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO9081854.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(3531..4415)
                     /gene="nlpI"
                     /locus_tag="ACHKIK_000624"
     CDS             complement(3531..4415)
                     /gene="nlpI"
                     /locus_tag="ACHKIK_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO9081855.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(4524..6659)
                     /gene="pnp"
                     /locus_tag="ACHKIK_000625"
     CDS             complement(4524..6659)
                     /gene="pnp"
                     /locus_tag="ACHKIK_000625"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO9081856.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(6906..7175)
                     /gene="rpsO"
                     /locus_tag="ACHKIK_000626"
     CDS             complement(6906..7175)
                     /gene="rpsO"
                     /locus_tag="ACHKIK_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO9081857.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(7324..8268)
                     /gene="truB"
                     /locus_tag="ACHKIK_000627"
     CDS             complement(7324..8268)
                     /gene="truB"
                     /locus_tag="ACHKIK_000627"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO9081858.1"
                     /translation="MSRPRRRGRDINGVLLLDKSQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(8268..8669)
                     /gene="rbfA"
                     /locus_tag="ACHKIK_000628"
     CDS             complement(8268..8669)
                     /gene="rbfA"
                     /locus_tag="ACHKIK_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO9081859.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(8833..11505)
                     /gene="infB"
                     /locus_tag="ACHKIK_000629"
     CDS             complement(8833..11505)
                     /gene="infB"
                     /locus_tag="ACHKIK_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO9081860.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(11530..13017)
                     /gene="nusA"
                     /locus_tag="ACHKIK_000630"
     CDS             complement(11530..13017)
                     /gene="nusA"
                     /locus_tag="ACHKIK_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO9081861.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(13045..13497)
                     /gene="rimP"
                     /locus_tag="ACHKIK_000631"
     CDS             complement(13045..13497)
                     /gene="rimP"
                     /locus_tag="ACHKIK_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO9081862.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(13704..13780)
                     /locus_tag="ACHKIK_000632"
     tRNA            complement(13704..13780)
                     /locus_tag="ACHKIK_000632"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13744..13746),aa:Met,seq:cat)
     gene            14128..15471
                     /gene="argG"
                     /locus_tag="ACHKIK_000633"
     CDS             14128..15471
                     /gene="argG"
                     /locus_tag="ACHKIK_000633"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO9081863.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(15479..17104)
                     /gene="yhbX"
                     /locus_tag="ACHKIK_000634"
     CDS             complement(15479..17104)
                     /gene="yhbX"
                     /locus_tag="ACHKIK_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO9081864.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(17563..17649)
                     /locus_tag="ACHKIK_000635"
     tRNA            complement(17563..17649)
                     /locus_tag="ACHKIK_000635"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17613..17615),aa:Leu,seq:gag)
     gene            complement(17664..17996)
                     /gene="secG"
                     /locus_tag="ACHKIK_000636"
     CDS             complement(17664..17996)
                     /gene="secG"
                     /locus_tag="ACHKIK_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO9081865.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(18224..19561)
                     /gene="glmM"
                     /locus_tag="ACHKIK_000637"
     CDS             complement(18224..19561)
                     /gene="glmM"
                     /locus_tag="ACHKIK_000637"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO9081866.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(19554..20402)
                     /gene="folP"
                     /locus_tag="ACHKIK_000638"
     CDS             complement(19554..20402)
                     /gene="folP"
                     /locus_tag="ACHKIK_000638"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO9081867.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(20492..22426)
                     /gene="ftsH"
                     /locus_tag="ACHKIK_000639"
     CDS             complement(20492..22426)
                     /gene="ftsH"
                     /locus_tag="ACHKIK_000639"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO9081868.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(22526..23155)
                     /gene="rlmE"
                     /locus_tag="ACHKIK_000640"
     CDS             complement(22526..23155)
                     /gene="rlmE"
                     /locus_tag="ACHKIK_000640"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO9081869.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            23281..23574
                     /gene="yhbY"
                     /locus_tag="ACHKIK_000641"
     CDS             23281..23574
                     /gene="yhbY"
                     /locus_tag="ACHKIK_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO9081870.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(23730..24206)
                     /gene="greA"
                     /locus_tag="ACHKIK_000642"
     CDS             complement(23730..24206)
                     /gene="greA"
                     /locus_tag="ACHKIK_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO9081871.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            24454..25887
                     /gene="dacB"
                     /locus_tag="ACHKIK_000643"
     CDS             24454..25887
                     /gene="dacB"
                     /locus_tag="ACHKIK_000643"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO9081872.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(25927..27099)
                     /gene="cgtA"
                     /locus_tag="ACHKIK_000644"
     CDS             complement(25927..27099)
                     /gene="cgtA"
                     /locus_tag="ACHKIK_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO9081873.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRIIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(27115..28080)
                     /gene="yhbE"
                     /locus_tag="ACHKIK_000645"
     CDS             complement(27115..28080)
                     /gene="yhbE"
                     /locus_tag="ACHKIK_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO9081874.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYALCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(28207..28464)
                     /gene="rpmA"
                     /locus_tag="ACHKIK_000646"
     CDS             complement(28207..28464)
                     /gene="rpmA"
                     /locus_tag="ACHKIK_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO9081875.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(28485..28796)
                     /gene="rplU"
                     /locus_tag="ACHKIK_000647"
     CDS             complement(28485..28796)
                     /gene="rplU"
                     /locus_tag="ACHKIK_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO9081876.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            29055..30026
                     /gene="ispB"
                     /locus_tag="ACHKIK_000648"
     CDS             29055..30026
                     /gene="ispB"
                     /locus_tag="ACHKIK_000648"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO9081877.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            30253..30531
                     /gene="sfsB"
                     /locus_tag="ACHKIK_000649"
     CDS             30253..30531
                     /gene="sfsB"
                     /locus_tag="ACHKIK_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO9081878.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(30579..31838)
                     /gene="murA"
                     /locus_tag="ACHKIK_000650"
     CDS             complement(30579..31838)
                     /gene="murA"
                     /locus_tag="ACHKIK_000650"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO9081879.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(31893..32147)
                     /gene="ibaG"
                     /locus_tag="ACHKIK_000651"
     CDS             complement(31893..32147)
                     /gene="ibaG"
                     /locus_tag="ACHKIK_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO9081880.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(32307..32600)
                     /gene="mlaB"
                     /locus_tag="ACHKIK_000652"
     CDS             complement(32307..32600)
                     /gene="mlaB"
                     /locus_tag="ACHKIK_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO9081881.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(32600..33235)
                     /gene="mlaC"
                     /locus_tag="ACHKIK_000653"
     CDS             complement(32600..33235)
                     /gene="mlaC"
                     /locus_tag="ACHKIK_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO9081882.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(33254..33805)
                     /gene="mlaD"
                     /locus_tag="ACHKIK_000654"
     CDS             complement(33254..33805)
                     /gene="mlaD"
                     /locus_tag="ACHKIK_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO9081883.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(33810..34592)
                     /gene="mlaE"
                     /locus_tag="ACHKIK_000655"
     CDS             complement(33810..34592)
                     /gene="mlaE"
                     /locus_tag="ACHKIK_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO9081884.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(34600..35409)
                     /gene="mlaF"
                     /locus_tag="ACHKIK_000656"
     CDS             complement(34600..35409)
                     /gene="mlaF"
                     /locus_tag="ACHKIK_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO9081885.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            35619..36596
                     /gene="yrbG"
                     /locus_tag="ACHKIK_000657"
     CDS             35619..36596
                     /gene="yrbG"
                     /locus_tag="ACHKIK_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO9081886.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            36610..37596
                     /gene="kdsD"
                     /locus_tag="ACHKIK_000658"
     CDS             36610..37596
                     /gene="kdsD"
                     /locus_tag="ACHKIK_000658"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO9081887.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            37617..38183
                     /gene="kdsC"
                     /locus_tag="ACHKIK_000659"
     CDS             37617..38183
                     /gene="kdsC"
                     /locus_tag="ACHKIK_000659"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO9081888.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCVLTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            38180..38755
                     /gene="lptC"
                     /locus_tag="ACHKIK_000660"
     CDS             38180..38755
                     /gene="lptC"
                     /locus_tag="ACHKIK_000660"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO9081889.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            38724..39281
                     /gene="lptA"
                     /locus_tag="ACHKIK_000661"
     CDS             38724..39281
                     /gene="lptA"
                     /locus_tag="ACHKIK_000661"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO9081890.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            39288..40013
                     /gene="lptB"
                     /locus_tag="ACHKIK_000662"
     CDS             39288..40013
                     /gene="lptB"
                     /locus_tag="ACHKIK_000662"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO9081891.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            40061..41494
                     /gene="rpoN"
                     /locus_tag="ACHKIK_000663"
     CDS             40061..41494
                     /gene="rpoN"
                     /locus_tag="ACHKIK_000663"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO9081892.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            41517..41804
                     /gene="hpf"
                     /locus_tag="ACHKIK_000664"
     CDS             41517..41804
                     /gene="hpf"
                     /locus_tag="ACHKIK_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO9081893.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            41922..42413
                     /gene="ptsN"
                     /locus_tag="ACHKIK_000665"
     CDS             41922..42413
                     /gene="ptsN"
                     /locus_tag="ACHKIK_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO9081894.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            42459..43313
                     /gene="rapZ"
                     /locus_tag="ACHKIK_000666"
     CDS             42459..43313
                     /gene="rapZ"
                     /locus_tag="ACHKIK_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO9081895.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            43310..43582
                     /gene="npr"
                     /locus_tag="ACHKIK_000667"
     CDS             43310..43582
                     /gene="npr"
                     /locus_tag="ACHKIK_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO9081896.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            43796..44428
                     /gene="yrbL"
                     /locus_tag="ACHKIK_000668"
     CDS             43796..44428
                     /gene="yrbL"
                     /locus_tag="ACHKIK_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO9081897.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(44425..45153)
                     /gene="mtgA"
                     /locus_tag="ACHKIK_000669"
     CDS             complement(44425..45153)
                     /gene="mtgA"
                     /locus_tag="ACHKIK_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO9081898.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(45150..45803)
                     /gene="elbB"
                     /locus_tag="ACHKIK_000670"
     CDS             complement(45150..45803)
                     /gene="elbB"
                     /locus_tag="ACHKIK_000670"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO9081899.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(46033..48369)
                     /gene="arcB"
                     /locus_tag="ACHKIK_000671"
     CDS             complement(46033..48369)
                     /gene="arcB"
                     /locus_tag="ACHKIK_000671"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO9081900.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(48465..49394)
                     /gene="yhcC"
                     /locus_tag="ACHKIK_000672"
     CDS             complement(48465..49394)
                     /gene="yhcC"
                     /locus_tag="ACHKIK_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO9081901.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            50069..54529
                     /gene="gltB"
                     /locus_tag="ACHKIK_000673"
     CDS             50069..54529
                     /gene="gltB"
                     /locus_tag="ACHKIK_000673"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO9081902.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            54542..55960
                     /gene="gltD"
                     /locus_tag="ACHKIK_000674"
     CDS             54542..55960
                     /gene="gltD"
                     /locus_tag="ACHKIK_000674"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO9081903.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNPLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            56144..57191
                     /gene="yhcG"
                     /locus_tag="ACHKIK_000675"
                     /pseudo
     CDS             56144..57191
                     /gene="yhcG"
                     /locus_tag="ACHKIK_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcG family protein"
     gene            complement(57251..57715)
                     /gene="nanQ"
                     /locus_tag="ACHKIK_000676"
     CDS             complement(57251..57715)
                     /gene="nanQ"
                     /locus_tag="ACHKIK_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO9081904.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(57712..58587)
                     /gene="nanK"
                     /locus_tag="ACHKIK_000677"
     CDS             complement(57712..58587)
                     /gene="nanK"
                     /locus_tag="ACHKIK_000677"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO9081905.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(58584..59273)
                     /gene="nanE"
                     /locus_tag="ACHKIK_000678"
     CDS             complement(58584..59273)
                     /gene="nanE"
                     /locus_tag="ACHKIK_000678"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO9081906.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(59321..60811)
                     /gene="nanT"
                     /locus_tag="ACHKIK_000679"
     CDS             complement(59321..60811)
                     /gene="nanT"
                     /locus_tag="ACHKIK_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO9081907.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(60921..61814)
                     /gene="nanA"
                     /locus_tag="ACHKIK_000680"
     CDS             complement(60921..61814)
                     /gene="nanA"
                     /locus_tag="ACHKIK_000680"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO9081908.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(61936..62727)
                     /gene="nanR"
                     /locus_tag="ACHKIK_000681"
     CDS             complement(61936..62727)
                     /gene="nanR"
                     /locus_tag="ACHKIK_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO9081909.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        15382 a        17086 c        15520 g        14874 t
ORIGIN      
        1 ctgataagcg aagcgcatca ggcattgtgt aggcagcaga aatgtcggat aaggcaccgc
       61 tgattactga tacaccggca gtaaattaaa gctcgataaa atatggacca gtgcgttgcc
      121 aacgccaaac accaaaatca gcgtaatcat cggcttgccg ccccagacgc ggaatttcgg
      181 gctgccaaag cgtttacgcg atgcgcgggc taacagcgcc ggaacaattg ccgcccagat
      241 ggtagctgct aaaccagcat aaccaatggc gtacaggaat ccgttcggga acagtagacc
      301 acccacaaca ggcggggcaa aggtcagcaa tgccgttttc aagcgcccca gggctgagtc
      361 atcgaaacca aacagatctg ccagatagtc aaacaaaccc agcgttacgc caaggaacga
      421 actcgctacc gcaaagtttg agaacacgac cagcagaagg tccaggctac ggctgttcag
      481 cacgccgctt aatgcctgta ccagcacatc aatattaccg cccttctctg caataccgat
      541 aaactccgga cgcgggatgt tacccatcgt cgccagcaac cagatggtat acagcgccag
      601 tgccatcagc gtaccgtaca ccaggcattt cacgatggtt ttcggatctt tgccgtaata
      661 cttcatcagg cttggcacgt taccgtgata accaaacgac gccaggcaga acggcaatgt
      721 catcagcaga tacggcgcat aagatgcatt gctttctgca acgttgaaca atgtcgcagg
      781 ctgcacatgc cccaacaggc tgccaaaggt gaggaagaag gtaatgactt tcgcccccag
      841 cacaatcgcc gtcatgcggc tgacggcctt agtactcaac cacaccacaa acgctaccag
      901 cagtgcaaaa ccaaaacccg ccgcccgtgc cgggacgttt agcgacatct ctgcgaaggt
      961 gtgatgcaga attgaaccac tggcagaaat ataggcatag gtcaggatat agagcacaaa
     1021 ggcaatggaa ataccgttga ccacgttcca gcctttgccc agcaaatctt tggtgatggt
     1081 gtcaaaactc gaaccgattc ggtaattcag gttagcttcc agaatcatca agccggaatg
     1141 cagcatacag aaccaggtaa agatcagcgc cgccattgac cagaaaaacc acgccccgga
     1201 catgaccact ggcagagaaa acatccctgc gccaataatg gtgccgccga taatcaccac
     1261 gccgccaagc agcgacggtg acgtttgggt ggtggttagt gttgccatga gggcttctct
     1321 ccagtgaaaa atagtgcgac tgcgttgtta tgcattgcac tgtaccagta cacgagtaca
     1381 aaagacagaa aaaaagcccc gatggtaaaa atcggggctg tatatattat tttacagatt
     1441 gtgttcgctg ttcagcgatg attacgcatc accaccgaaa cgacgacgac cggtagagtc
     1501 atcacgacgc ggagcgcggc cttcacgacg ttcgccgcta aaacgacgac catcaccacg
     1561 gccaccttca cggcgttcac cgctgaagtt acgaccgcct tcacgacgtt cgccaccgaa
     1621 accacgacca ccgccacgac gctcaccgcc agtatgcggc tgcgcatcgc ccagcaactg
     1681 catattcatc ggcttgttca gaatgcgagt acgcgtaaag tgttgcagta cttcacccgg
     1741 catacctttc ggcagttcga tagtggagtg agaagcaaac agcttaatgt taccgatgta
     1801 acggctgctg atgtcgcctt cgttagcaat cgcgccaacg atatgacgaa cttcaacacc
     1861 atcatcgcgg cccacttcaa tgcggtaaag ctgcatgtca ccaacatcac gacgttcacg
     1921 acgcggacga tcttcacggt cgccacgtgg gccacggtcg ttacgatcgc gcggaccacg
     1981 gtcatcacgg tcacggaatt cacgtttcgg acgcatcggc gcatctggcg gaacaatcag
     2041 agtacgttca ccctgtgcca ttttcagcag tgccgcagcc agagtttcga gatcctgctc
     2101 ttcaccttct gcagtcggct gaattttgct cagcagtgcg cggtattgat ccagatcgct
     2161 gctttccagc tgctgctgta ctttagcggc gaatttttcc agacggcgtt tgcccagcag
     2221 ttctgcgttc ggcagttcta cttccggaat agtcagcttc atagtacgtt caatgttgcg
     2281 cagcagacga cgctcgcggt tctcaacgaa cagcagcgcg cggccagcac gacccgcacg
     2341 accggtacga ccgatacggt gaacgtaaga ctcagaatcc atcgggatat cgtagttaac
     2401 taccaggctg atacgctcaa cgtccaggcc acgggctgca acgtcggtcg caatcaggat
     2461 gtccagacga ccatctttca ggcgttccag tgtctgttca cgcagcgcct ggttcatgtc
     2521 accgttcagc gcggcgctgt tgtagccgtt acgctcaaga gcttcagcca cttccagagt
     2581 cgcgtttttg gtacgaacga agataatcgc cgcatcaaaa tcttccgctt ccaggaaacg
     2641 taccagtgct tcgtttttgc gcatacccca gacagtccag tagctctggc tgatgtcagg
     2701 acgggtagtc acgctggact gaatgcgcac ttcctgcggc tctttcataa agcggcgggt
     2761 aatgcgacga atcgcttccg gcatggttgc agagaacaga gcggtctgat gaccttccgg
     2821 gatctgcgcc ataatggttt caacgtcttc gatgaagccc atgcgcagca tttcgtcagc
     2881 ttcatccaga accagaccgc tcagtttaga gaggtccaga gtgccacgtt tcaggtggtc
     2941 cagcagacgg cccggagtac cgacaacgat ctgcggcccc tgacgcaggg cgcgtaattg
     3001 cacgtcataa cgctggccgc cgtatagagc aaccacattt acgccgcgca tgtgtttaga
     3061 gaaatccgtc attgcttcag caacctgtac cgccagttcg cgggtcggtg ccagcaccag
     3121 aatctgtggt gctttcagct caggatcaag attctgcaac agaggtaaag agaatgctgc
     3181 agtttttccg ctccccgtct gggccatacc cagaacgtcg cggccattca gcagatgtgg
     3241 aatacactct gcctgaattg gagatggttt ttcgtaaccc agatcgttaa gggcttcaag
     3301 gataggagcc ttcaggccca gatctgcaaa agtggtttcg aattcagcca tgtagtacgt
     3361 gtgcctcaaa attaatggcg gccagtctac ataactcatc atgaaattga tcagcaattt
     3421 tcattgaaaa gtgtgaaccg gctcaaagta ggtgtattaa cgaacaacaa cgccctcacc
     3481 cgttaaggtg atggcaatca aaaaagatta cgggctgatg tgtacgtcag ctattgctgg
     3541 tccgattctg ccaggtcatc ttggtcctgg cccaggagcg ataattccaa caatgcgtat
     3601 cggtgctcaa caaagttatg aacgttgttg gcaaccgcca gtttgaacag tgccgtggcg
     3661 ctgtccaaat cccccagact taggtagtac ttacctaaat agaagttggt ttcactgaga
     3721 tgctcagcga gcgaggtgtt atccgttgcg tccgccttga gcctttccat taacgtttgt
     3781 tcgctaatgt tgcccaggta gaactcgaca atgttccatc cccactgttc cttatccgat
     3841 ttttcgaagt gctgtttcaa cacttcttta gcctgcttct catcgagctt ctgctcggcg
     3901 agataaagcc acagactacg gaaaggatca ttgggatcgt cttgataaaa cgccagcaga
     3961 tcatcttgcg ctaacttgtc acgaccgccg taatataatg cgatcccgcg attcaagtgc
     4021 gcgtagttgt aagttggatc aagctcaagt acagaatcaa acgcttcata ggcagcatca
     4081 aaattgcctg cctgcgttaa atatatgcct aagtaattga atacttcagg catatccggt
     4141 cggattgcca gcgcttgcga aaaatcgtta cgcgctaatg ccctcagacc gagactatca
     4201 tacaacactc cgcgctcata taaaagctgt gcgcgttcgt catcggttaa agcccgactg
     4261 gcaaggattt gttccatacg tgccagaatc acttcctgct gtaaagtcgg ttgcaatggt
     4321 accgcgagga cttcactttt acgccaggaa gtattactgc atcctgcaag cgtaagtgct
     4381 gtcgcaacga aacaccagcg caaaaaaggc ttcatttccc actcccgaag accacggttg
     4441 aatgaacgtc ctgttcccgg ttgctaacaa ggcgtcctgc ccggttaaaa gccccccgcc
     4501 gcagcggagg gcaaatggca accttactcg ccctgttcag cagccggagc ttccggtgct
     4561 gcagcaggtt gagactgctc agtcgcttct ttaatgctca gacggatacg gccctggcga
     4621 tcaacttcca gaactttcac cggtacttcc tgacccatct gcaggtaatc ggtcactttc
     4681 tcaacgcgtt tgtcagcgat ttgagagatg tggaccagac cttctttacc gccgccgatg
     4741 gcaacaaatg cgccaaagtc aacgatacgg gtcactttac cagtgtagac gcggcccact
     4801 tcgatttctg cagtgatctc ttcgatacga cgaatagcat gtttcgcttt ctcgccgtcg
     4861 gtcgctgcga tcttcacagt accgtcatct tcgatttcga tggtagtgcc agtttcttcg
     4921 gtcagggcac ggataacaga gccgccttta ccgataacgt ctttgatctt gtccgggttg
     4981 atcttgatgg tatggatacg cggtgcgaac tcagagatat cgccacgcgg cgcgttgatc
     5041 gcctgttcca ttacgcccag gatatgcaga cgcgcacctt tagcctggtt cagcgcaacc
     5101 tgcatgatct ctttggtgat accttcaatt ttgatatcca tctgcaatgc agagataccc
     5161 tcgcgggaac ccgcaacttt gaagtccata tcgcccaggt gatcttcgtc gcccaaaatg
     5221 tcagacagta caacgtagtt gtcgccttct ttcaccagac ccattgcgat acccgcaacg
     5281 gcagctttga tcggcacacc tgcgtccatc agcgccagag acgcgccgca cacggaagcc
     5341 atagaagagg aaccgttgga ttcagtgatt tcagacacaa cacgtacggt gtacgggaat
     5401 ttgtccatat ccggcatgac tgccagcacg ccgcgcttcg ccagacgacc gtgaccaatt
     5461 tcacgacgct tcggagaacc gaccatgccg gtttcgccta cggagtacgg agggaagttg
     5521 tagtggaaca ggaaggtatc ggtacgttcg cccatcagtt catcaagaac ctgcgcgtca
     5581 cgagcagtac ccagcgttgc ggtaaccagc gcctgcgttt caccacgggt gaacagcgca
     5641 gaaccgtgag tacgcggcag cacgccagta cgcacatcca gaccacggat catatctttt
     5701 tcacgaccgt cgatacgcgg ttcgcctgcc agtacgcggc tacgaacaac gtttttctcg
     5761 atagcgtgca gaatttcacc cagttcgttt tcgtccaggg tttcgtcttc agcaagcagc
     5821 gtcgcgatgg tttcagattt gatgacatca acctgcgcat aacgctcttg tttgtcggtg
     5881 atgcggtaag catcgctcag gcgagcttca gccagtgcag caacgcgcgc gttcagcgct
     5941 tcgtttaccg gctccggctg ccagtcccaa cgtggtttac cggcttcttt caccagttca
     6001 ttgatgttct gaataacaac ctgctgttgt tcatgaccga acactactgc gcccagcatc
     6061 tggtcttcgc tcagcagttc agcttcagat tcaaccatca gaacagcggc ttcagtaccg
     6121 gcaacaacca gatccagttt gctctctttc agctcgtcct gagtcgggtt cagtacgtac
     6181 tggtcattga tgtaacctac gcgagcagca ccaatcggac cattgaacgg aataccagac
     6241 agagacagtg ctgcggaagc accaatcatc gcgacgatat ccgggttaac ttgcgggtta
     6301 acagaaacca cggtggcgat aacctgaact tcgttgacga agccttccgg gaacagcggg
     6361 cgaatcgggc ggtcaatcag acgcgcgatc agggtttcgc cttcgcttgg gcggccttca
     6421 cgacggaaga agctacccgg gatacgacca gcagcgtagg tacgctcctg atagttaacg
     6481 gtcagtggga agaagtcctg acctggtttg gcttttttct ggccaacaac ggtaacaaat
     6541 accgcggtgt catccatgct aaccataaca gcggcagtag cctgacgagc catcatgccg
     6601 gtttccagag tcacggtgtg ttggccgtac tggaatttac gaacgatcgg attaagcaat
     6661 gtaatatcct ttctctttct tagacagtac cttacggcac tggtgttaat acccgatctt
     6721 ctgcgcatcc tcgcgactaa tgacaaccct aacccagctc tatgtgggta aagcctctca
     6781 ttagccgcgc gaacctctgc aacggaagat cattcatagc aacaatacat tagtttccag
     6841 tgaattgctg ccgtcagctt gaaaaaaggg gccactcagg cccccttttc tgaaactcgc
     6901 aagaattagc gacgcagacc caggcgctcg atgagctggg tgtaacgtgc tacgtcttta
     6961 cgtttcaggt agtcgagcag tttacgacgc tgagaaacca tgcgcagcag accacgacgg
     7021 ctgtggtgat cttttttgtg ctctgcaaag tggccctgca ggtggttgat ctgtgcagtc
     7081 agcagtgcta cctgaacttc ggtagaaccg gtgtcgtttg cgtcacgacc aaactcagaa
     7141 acgattttag ctgttgcttc agtacttaga gacattttaa aactccaaag tatatagaat
     7201 gaaaggacgc cgatctctaa ttcagcgatc ccagtgtacg ttacgcaaag tgttaaacaa
     7261 tttacgcgac gttaagcggc agtattctac tcgtagcgac ctgttatcgc aagacggtta
     7321 acattacgcc gggtattcaa ccaccaggcg acgaggcgca acgcggcctt catcgtcaat
     7381 ttcgcccata ccgataaatt tgccgttctc accttctgta acgcgaacca gtccttccag
     7441 cggcgcaccg gatgtacgaa ctgggttacc atttttgaag taaacagaag acgttaacgg
     7501 aagattcacc accgggtagt ccgaagctgg actgtccatt ggcatcagta atggatcaag
     7561 taactccgca gccggaatat cctgctgttc ggcttgttcg accagttcac gcagatgttc
     7621 cagggtcacc atccgttcaa ccggatattt acttaccgcc agacgacgca ggtaaataac
     7681 atgcgcgcca caaccgagtt tttcacccag gtcatcaatg atggtgcgga tataagtgcc
     7741 ttttgagcag tgaatttcca gctccagctc attgccttcg tggcgaataa acagcaattc
     7801 ataaacggta atcgggcgcg cttcacgcgg tacttcaatg ccctgacgcg catattcgta
     7861 cagtttttta ccctgatatt tcagtgctga atacatcgaa gggatctgtt cgatatcgcc
     7921 acggaaagta tccagcgccg ccgccagttg ctctgcgcta aaggttaccg gacgttcttc
     7981 aacgatttgt ccgtcggcat cagaagtatc ggtacgctgt ccaagacgcg caatgacccg
     8041 atagcgtttg tcggagtcca gcagatactg ggaaaacttc gtcgcttccc cgaggcaaat
     8101 tggcaacatg ccagttgcca gcgggtccag cgcaccggta tgcccggcac ggttggcgtt
     8161 ataaatccgt ttcacttttt gcagcgcatc attgctggac ataccctgcg atttatccag
     8221 caacaaaacg ccgttaatgt cgcgaccgcg acgacgagga cgactcatta gtcctccttg
     8281 ctgtcgtccg ggttaacacg acgttcttcg tcatgtttga ccacgctggt caccaggttt
     8341 gacatgcgca tcccttcaac cagagagttg tcgtagaaga aggtcagttc cggcacgata
     8401 cgcaggcgca tcgctttccc cagcaggctg cggatgaaac cagaagcttc ttgcaacgct
     8461 ttgatgcccg ctttaaccgc gtcttcatct ttgtcgttga ggaacgtcac atatactttg
     8521 gcatacgcca ggtcgcgaga catttcgaca ccggaaacgg tggtcatcat gcccaggcga
     8581 ggatctttaa tttcacgctg caggatgaga gcgatctctt tttgcatttc ctgcgctacg
     8641 cgctgcgggc gaccaaattc tttcgccata ataaattctc ctgacaaaaa aggggctgtt
     8701 agcccctttt taaaattaat ttcaggtgga agggctgttc acgttggcct gataagacgc
     8761 gccagcgtca catcaggcaa tccatgccgg atgcggcata aacgccttat cccgcatgga
     8821 accctaaaaa ccttaagcga tggtacgttg gatctcgatg atttcgaata cttcgatcac
     8881 atcgccagtg cggacgtcgt tgtagttctt aacgccgata ccacattcca taccgttacg
     8941 gacttcgtta acgtcatctt tgaagcggcg cagggactcc agctcgcctt cgtagataac
     9001 cacgttgtca cgcagaacgc ggatcgggtt gtgacgttta accacacctt cggtaaccat
     9061 acagcctgcg atggcaccaa atttcggcga tttgaacacg tcacgaactt ccgccagacc
     9121 gataatctgc tgtttcagtt ccggagacag cataccgctc atcgccgctt tcacttcgtc
     9181 aatcaggtta tagatgacgg agtagtaacg cagatccagg ctttccgctt caatcacttt
     9241 acgtgcagag gcatcagcac gtacgttgaa gccaaccagg atggcgttgg acgccgcagc
     9301 cagggtggcg tcggtttcgg tgataccacc tacgccagaa ccgatgatct tcactttaac
     9361 ttcgtcagta gacagtttca gcaaggagtc ggagatcgct tccacagaac cctgtacgtc
     9421 tgccttcagg acgatgttca cttcgtgaac ttcgccttcg gtcatgttgg cgaacatgtt
     9481 ctcgagttta gatttctgct gacgcgccag tttaacttcg cggaatttac cctgacgata
     9541 cagtgcaact tcacgcgctt tcttctcgtc acgtacaacg gtaacttcat cacccgcagc
     9601 cggtacgccg gacaggccga ggatttccac cggaatggac ggacccgctt ccagcacttc
     9661 ctgacccagt tcgttacgca tcgcacgaac acgaccgtat tcgaagccac acagaacgat
     9721 atcgcccttg tgcagagtac cttcacgtac cagaacggta gcaaccggac cacgaccttt
     9781 atcgaggaag gattcgataa ccgcaccgct cgccatacct ttacgtaccg ctttcagctc
     9841 cagaacttcc gcctgcagca ggatagcgtc cagcagttca tcgatgccgg tacccgcttt
     9901 cgcagatacg tgtacgaact ggctttcacc gccccactct tccggcagga tgccatactg
     9961 ggagagttcg ttcttaacac gatccggatc ggcttctggt ttatcaatct tgttcactgc
    10021 aaccaccacc ggtacctgcg ccgctttcgc gtgctggatt gcttcgatgg tctgcggcat
    10081 cacgccgtcg tcggcagcaa caaccaggac tacgatgtcc gttgcctgcg caccacgtgc
    10141 acgcattgaa gtaaacgcgg cgtgccccgg ggtgtccagg aaggtgatca tgccgttttc
    10201 agtttcaacg tggtatgcac caatgtgctg ggtaatgccg cccgcttcgc cagaggcaac
    10261 tttcgttgaa cgaatgtagt ccagcagaga ggttttaccg tggtcaacgt gacccatgat
    10321 ggtcacaacc ggcgcgcgcg gttcagccgc agcacccgtg tcacggtcgc tcattaccgc
    10381 ctcttccagc tcgttttcac gacgcaggat aactttgtgg cccatctctt cagcaaccag
    10441 ctgtgcggtt tcctgatcga taacctggtt gatggttgcc attgcgccca gtttcatcat
    10501 cgctttgatg acctgagagc ctttaaccgc catcttgttc gccagttcgc caacggtgat
    10561 ggtttcgccg atcacaacgt cacggttaac ggcctgagct ggcttctgga agccttgctg
    10621 cagggaagaa cctttacgtt ttccgccttt accgccacgt actgctgcgc gtgcttcttc
    10681 acgatcagct tttgattcag cgtgtttgtt gcctttcttc ggacgcgctg ctttcgcgtt
    10741 acgaccacgg ccacgaccgc cttcgacttc acgatcgctt tcgtcttctg cctggcgagc
    10801 atgttgagaa gtagtgacgt gataatcgct ggaatcttca gtcggttccg cgttatcagt
    10861 ccatttgttt tcttccgcca tacgacgtgc ttcttcagca acgcgacgtg cttcttcttc
    10921 gagtttacga cgcgcttctt cttcagcttt acgcttgagc tctgcagctt cctgctcacg
    10981 gcgggctttt tcagcctggg cgtttttagt catatcgtct tgttgattgc tcactttgtc
    11041 tttttccgca gcttcacgtt tcgcttgttc agcagcttca cgcttagctt gttctgcggc
    11101 ctcacgttca gctttttgtt gcgcctcgcg tttagccgat tcttctgcct cacgacgggc
    11161 ttgctcttcc gcttcacgct gcgcttgctc ttccgctgca aggcgttcag cctcttgcgg
    11221 atcgcgtttc acaaaggtgc gtttcttgcg gacttcgatt tgtaccgatt tgctttttcc
    11281 accggtacca ggaatgttaa gggtgctgcg tgttttacgt tgcagcgtca atttgtccgg
    11341 gcctgaattt ttctgattca ggtggtcaat caaagtctgt ttctcttgtg cagacacaga
    11401 gtcgtcagca gacttccgga tacctgcatc agcaaattgc tgtaccaggc gttccacgga
    11461 ggtctgtcgc tctgcggcca gcgttttaat cgttacatct gtcatgctgt tccttcctgc
    11521 tacagtttat tacgcttcgt caccgaacca gcaaatatta cgggcagcca taatcagtgc
    11581 tccggctttt tcgtcggtca acccttcgat atcagccaga tcatcaatgc cctgttcggc
    11641 gagatcttcc agcgtacaaa cgccacgggc ggccagtttg aatgccaaat cacgatctat
    11701 cccttcaagg ttcagcagat cgtcagccgg tttgttatca ccgaggcttt cttcctgggc
    11761 ctgtgcaatg gtggccagtg catttttagc acgctcgcgc agtgcttcaa cggtcggctc
    11821 atcaaggcct tcgatttcca acagctcttt catcggcaca taggccagtt cttccagcgt
    11881 cgagaagcct tcttctacca gaacagtcgc gaagtcttcg tcgatgtcga gatatttggt
    11941 gaaggtgtcg atcgctgcgt gcgcttccgc ctgatgctta gcctgcaggt cgtcaacggt
    12001 catcacgttg agttcccaac cgctcagctg cgaagccaga cgcacgttct gaccgttacg
    12061 gccaatcgcc tgcgccaggt taccggcttc aacggcgata tccatggtgt gtttatcttc
    12121 atccaccacg atagaagcaa cgtctgccgg tgccattgcg ttaatcacaa actgcgccgg
    12181 gttatcatcc cacaggacga tatcgatacg ctcgccgccc agttcagtag aaaccgcctg
    12241 aacacgcgcg ccacgcatac ctacacaggc acctaccgga tcgatacgtt tatcgttggt
    12301 tttcaccgcg attttcgcac gagaacccgg atcgcgagcc gctgctttaa tttcaatcac
    12361 ttcttcgccg atttctggca cttcaatacg gaacagttcg atcagcattt ccggcttgga
    12421 acgagtgacg aacagttgcg cgccacgcgc ttccgggcga acggaataga gcacgccacg
    12481 aacgcggtcg ccagggcgga agttttcacg cggcagcata tcttcgcgca ggatcacggc
    12541 ttcagcattg ttgcccagat ccagagagat gttgtcgcgg tttacttttt tcaccacgcc
    12601 ggtgatgatt tcaccttcgt gttcacggaa ctgatcaacc accatcgcac gttcggcttc
    12661 acgcactttc tgcacgataa cctgttttgc cgtctgggta gtgatacggt caaaggtaac
    12721 agactcaatc tgatcttcaa cgtaatcgcc caggttcagg ctttcatctt cataacgtgc
    12781 ggcttcaagg gtgatttcct tggtcggctg ggtgacttca tcaacaacta accagcgacg
    12841 gaaggtgtca aaatcaccgc ttttgcgatc gatctgtacg cggacgtcga tctcttgttc
    12901 atattttttc tttgttgctg tcgccagcgc gctttccaat gcttcgaaaa tcttctcgcg
    12961 aggtagcgcc ttttcattgg atacggcttc aactacagcc aaaatttctt tgttcatcgc
    13021 gggcttttca cctcatccag actattaaaa gtggggaacc aggttcgcct tctggatatt
    13081 actcagcgcg aacacttcat ctttaccttc gacggtaact gtgatcattt caccgtctac
    13141 cgctttgata acgccctgcc atttacgacg gttttgtacc gccatacgga gaaccagagt
    13201 cacctcttct ccgacaaaac gggcgtagtg ttcagccgtg aacagtgggc gatcgagacc
    13261 cggtgaggag acttccaggt tataagcaac ggtgatggga tcttcaacat ccagcacagc
    13321 acttacctgg tggctcacat cagcacaatc atcaacattg atgccatctt cactatcaat
    13381 atagatgcgc agtgtggatg tgcgaccgcg aataaattcg atgccaacca gttcaaaacc
    13441 cagggcctca actggcgcag taatcatctc tgttaatttt tgctctaatg tggacaagcc
    13501 cacccccaag acataaaaaa agggcctaaa gcccagttat tctgtagtca gataacaaaa
    13561 aaccccgata aatcggggct ttatataact gaaccctata accgcaactg cggtctggag
    13621 cactttccag aaggattttt tcaaatccca ctacgaaggc cgaagtcttc acagtatatt
    13681 tgaaaaagga ctctaaggga aagtggttgc gggggccgga tttgaaccga cgaccttcgg
    13741 gttatgagcc cgacgagcta ccaggctgct ccaccccgcg cctgaaacgt ggcaaattct
    13801 actcgttttg ggtaaaagat gcaaatactg ctgggatttg gtgtaccgag acgggacgta
    13861 aaatctgcag gcattatagt gatccatgcc acattttgtc aacgtttatt gctaataacg
    13921 tgaatgaata tccagttcac tttcatttgt tgaatacttt tgccttctcc tgctctccct
    13981 taagcgcatt attttacaaa aaacacacta aactcttcct gtctccgata aaagatgatt
    14041 aaatgaaaac tcatttattt tgcataaaaa ttcagtgaga gcggaaatcc aggctcatca
    14101 tcagttaatt aagcagggtg ttattttatg acgacgattc tcaagcatct cccggtaggt
    14161 caacgtattg gtatcgcttt ttccggcggt ctggacacca gtgccgcact gctgtggatg
    14221 cgacaaaagg gagcggttcc ttatgcatat actgcaaacc tgggccagcc agacgaagag
    14281 gattatgatg cgatccctcg tcgtgccatg gaatacggcg cggagaacgc acgtctgatc
    14341 gactgccgca aacaactggt ggccgaaggt attgccgcta ttcagtgtgg cgcatttcat
    14401 aacaccaccg gcggcctgac ctatttcaac acgacgccgc tgggccgcgc cgtgactggt
    14461 accatgctgg ttgctgcgat gaaagaagat ggcgtgaata tctggggtga cggtagcacc
    14521 tataaaggaa acgatatcga acgtttctat cgttatggtc tgctgaccaa tgctgaactg
    14581 cagatttaca aaccgtggct tgatactgac tttattgatg aactgggcgg ccgtcatgag
    14641 atgtctgaat ttatgattgc ctgcggtttc gactacaaaa tgtctgtcga aaaagcctac
    14701 tccacagact ccaacatgct tggtgcaacg catgaagcga aggatctgga atacctcaac
    14761 tccagcgtca aaatcgtcaa cccgattatg ggcgtgaaat tctgggatga gagcgtgaag
    14821 atcccggcag aagaagtcac agtacgcttt gagcaaggtc atccggtggc gctgaacggt
    14881 aaaaccttta gcgacgacgt agaaatgatg ctggaagcta accgcatcgg cggtcgtcac
    14941 ggcctgggca tgagcgacca gattgagaac cgtatcatcg aagcgaaaag ccgtggtatt
    15001 tacgaagctc cggggatggc actgctgcac attgcgtatg aacgtctgtt gaccggtatt
    15061 cacaacgaag acaccattga gcagtatcac gcgcatggtc gtcagttggg ccgtctgctg
    15121 taccaggggc gttggtttga ctcccaggcg ctgatgctgc gtgactctct gcaacgctgg
    15181 gttgccagcc agatcactgg tgaagttacc ctggagctgc gccgtgggaa cgattattca
    15241 atcctgaata ccgtctcaga gaacctgacc tacaagccag agcgtctgac gatggaaaaa
    15301 ggcgactcgg tgttctcgcc agatgatcgt attggtcaat tgaccatgcg taatctggat
    15361 atcactgata cccgagagaa actttttggt tatgccaaaa ctggcctgct ctcctcctct
    15421 gccacttcag gcgtgccgca ggtggagaat atggaaaaca aaggccagta attcgacatc
    15481 aaccctgccc ttcaggggtg gggttacctt gcttatcctc tgtaaactcc tttctgagca
    15541 tcacataatc gaaaacatca tggtttgcat ccacaacccg tgagtctcct ttatagtgtg
    15601 caatcacttc ctcaagcgtt tgcggctgtt ccggctttgt tacccccatc cacgcattaa
    15661 taaggtaatt attgtaagct gtcgaaaaga tgttgttttc cgttgtgcga tccacgccat
    15721 ccccaagaac ggggctatac cagataaaca tcgggacatg atatgcctgc tggctagcct
    15781 ccctgcctcc atgaaaatag acgttcttct tcgtagggtc acgttccagg ccgtggtcgg
    15841 caaaatacat gaccgaggcg cggcgatctt ttaataattc aaaaacctga cctagcaaac
    15901 tatctgtgta atgaatggag ttgtcatagc aggcatcctg atcgtcctgc ggttgaaaca
    15961 cggcgctgga ttgcggatag gcgctacaag ccggttcatg gcttccattt aaatgaagaa
    16021 caatcagttt cttttgctgc gtattttgct gtaatgcttg cgataaatgc ggcaacaaca
    16081 attcatcaaa tcctctgaca taaactgttt ccatggcgcg catggcgata ctggtaactg
    16141 ctgtaccatt ctgccgaaaa gcggattgcg agcttagcca gaaagtctga aatcctgctt
    16201 gattagccat attaataatg ttgtcggggt aattatgaat gtcatgactc aaaacagaat
    16261 cagcagttaa agaaagggga accgacagcg cagtgtaagg tgcgccgctt attgcttgat
    16321 taaacagttt gatctgtttt ctttgtgctt caacttgcgg tgtcgtagag cgtgtatatc
    16381 catacaaaga catattgtcg acacgtacag actcccccac aatcaacacg taggtatcaa
    16441 tacctgtatc cctgactgat aattgaaaat acggcaccgt gtttgcaatt gagagtaatc
    16501 tttgatgctc tttcgctgct aaagcaaaat agttgagatt gaaaaacggc gtataggtag
    16561 caaatcgcga cgctagtata tatggactga acgctttttt attttttgca tctttataag
    16621 caaattgaca agcggaaaat aaactgcccg aaatgacaat cagcaataat attcctgtca
    16681 ctttttttgt cggcaaggaa acatcatatt ttattattac tgccaaaaaa agaagggata
    16741 aaaaagcaaa ggcacataga taagggatat acatccccag cattttgaca acttcatccg
    16801 gatcactctg gagcacacta atcgcaaatc catcattgaa agttgtacca aaggtacacc
    16861 atgaatagag gctaatactc atgtctgccg cagtcagtaa cacaaatggt gcggcaacaa
    16921 gcaggcgaag cggaagggat gatatacgtt ttacaaccag gatggtcagg acgaagaaca
    16981 gcaatcgctg aaccatatgc gcgccggaag aggcgactaa gttcgtaata ccaaaaacaa
    17041 taacacaaag atacaacaac caatgagatt taaaacttct agcaaatttg ttgaatactg
    17101 tcatccagaa atccaagttc acaaccaata taaacaatta tcaacaatgt tcaattttat
    17161 atcagacatg tttttcaact ctgatccata tcagaccgaa acactgttag ttttttcttt
    17221 ttaaatgatg cggccattgc gttctcagta attaaataac attatcaatg cgtctctaaa
    17281 cattacgcag aacgtagcca tcagtagtta tactgacagg agcaggatgg tttatcgaga
    17341 agtaaacata tccgtattcg taccggaact ggagttgaca caataaagtg ccaattatgt
    17401 cagcagaagg gaaaagttta taactaaggc gtaccggcac catcgtttca aggtaccagc
    17461 tacgagtaaa gcaactggac gagatacaga tacctgacaa ccattcctca gaccaggacc
    17521 aaaacgaaaa aagacgcttt tcagcgtctc ttttctggaa tttggtaccg aggacgggac
    17581 ttgaacccgt aagccctatt gggcactacc acctcaaggt agcgtgtcta ccaattccac
    17641 cacctcggca cggatactac tttttagttc gggatatcgc tggtcggctt agccggagca
    17701 gctggttgag tttgttcggt tttcaccggt gcactcagat tttcccattc gctaccttta
    17761 ttggttttgt tgctattgat gttacccagc accagactga tgatgaagaa taacgttgcc
    17821 agcagcgccg tcatgcgggt catgaagtta ccagaaccac ttgaaccaaa cagcgtagcg
    17881 gaagcgcctg ctccgaagga ggctcccata tcagcgcctt taccttgctg cagcatgatc
    17941 agaccaacaa ggccaattgc cacaataagg aaaactacta aaagagcttc gtacataatc
    18001 aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa ccaataaagc gggagttttt
    18061 aatctttccc actgaagcgg gtgtgaatac taaccaaagc gaatgacctt cgcaagggca
    18121 attttatcgc attgtatcaa ctgcggaaaa aaacagcaaa accgatgtgt tcgctgagaa
    18181 aaaaggcgac aatcgccgcc tttttagcca gttatctaac gctttaaacg gcttttactg
    18241 catcggcgat gcggtgtgca aattcagtca cctgcgcttc gtcttcgcct tccaccatca
    18301 cgcgaattaa cggttcggtg ccggatttac gcagcaacac gcgtccacgg ttgcccagcg
    18361 cagcttcaac ctctgcggtc acggctttaa ctgactcatg ctcaagtgga tcgccgctac
    18421 ctgcggtgta acgtacgtta accagaatct gcgggaacat tttcatgccg ctgcaaaggt
    18481 cgtgcaggct catatggtta cgtgccatcg ccgccagcac ctgcaagcca gcaacgatgc
    18541 cgtcaccggt agtagtttta tccagtagga tcacatgacc ggaattctct gcaccgatac
    18601 gccagccttt ctcctgcatt ttttccagta cgtagcggtc gcccactttc gcgcgcgcaa
    18661 atggaattcc taactgtttc agcgccagtt caagccccat gttgctcatc aatgtaccca
    18721 cagcgccacc acgcagctgg ccctgacgaa gaccttcacg cgcgatgata tacatgatct
    18781 gatcgccatc gactttattg ccttcatggt caaccataat cacgcgatcg ccatcgccgt
    18841 cgaaggcaat accgagatcc gctttttcag ccagcacacg agcctggagc gcgcgaacgt
    18901 cggtagcccc cacttcggca ttgatgttta caccgtttgg ctcacaaccg atagcgataa
    18961 cgttcgcccc cagttcgcgc agcacgttcg gcgcgatgtg ataagtcgca ccgtttgcac
    19021 aatccaccac aatcttcagt tcactgaggc taagttcgtt cgggaacgtg gctttgcaaa
    19081 actcgatata gcgacccgcg gcatcaacga tacggctggc tttacccagt tctgccgaat
    19141 caacgcagct gatctccttt tccatttccg cttcgatggc ctcttctacc gcatccggca
    19201 gtttggtgcc gtcgatagag aagaatttaa tgccattatc gtagaacggg ttatgagatg
    19261 cagatatcac aattccggcc tctgcgcgga aggtacgcgt cagataagcc acggccggtg
    19321 ttggcatcgg gccagtgaag agtgcggaaa ggcccgctgc cgccagaccc gcttccagtg
    19381 ctgactccag catatagcca gaaatacgcg tgtctttacc aataataatc ttacgggagc
    19441 cgtggcgcgc cagcacttta cccgcggccc aacccagctt aagcacaaaa tcaggtgtga
    19501 tcggcgcatc ccctacacga ccacgaatcc catcggtacc gaaatattta cgattactca
    19561 tagcgtttgt tttcctttgc agacagagtg gcttccacca cccgcatcgc ttctacggtt
    19621 tctttgacgt catgaacacg aatgatgtgc gcgccttgca ttgcggcaat gaccgcacag
    19681 gccagactac cgctcaggcg ctcggacggc cccacgttca gcagctgccc aatcatcgat
    19741 tttcgtgaca tacccaccaa cagcggcagg ttgaaatggt gaaattcagc caggcgcgcc
    19801 agtaatgaat agttatggga gagattttta ccgaaaccga atccggggtc gagcaacaat
    19861 ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt gctcaataaa gtagcgattc
    19921 acttctgcaa agacatcgtc atacttcgga gcttcctgca tggtttttgg atttccctgc
    19981 atatgcatca gacaaaccgg taaaccggtt tctgcagccg cctccagagc gccaggttcg
    20041 gaaagggagc ggatatcatt aataatgtga gcgccaactt tcgctgactc acggatgact
    20101 tctggtttgg atgtatcgac cgagatccag acttcgaagc gttgagcaat tgcctcaacc
    20161 acaggaataa cacgttgcaa ctcttcttca acgctaactt ccgccgcccc tgggcgcgtg
    20221 gactcgccac caacgtcaat gatcgtcgcg ccagcattga tcatcagatt cgcatgtttc
    20281 accgcatcta tcagcgagtt atgcgtgcca ccatccgaaa aggaatcagg cgtgacgttg
    20341 aggatcccca ttacgtgagg atggctaagg tccagtgaag taccctgggc aaagagtttc
    20401 atgatgttat ccctagtatg aattgataag aaaaaacccc ggagcacgcc ccggggtttt
    20461 cggtacaaat acagtcatct gatgcgggaa cttacttgtc gcctaactgc tctgacatgg
    20521 tgttacccgg gttcggcgta cgcggttcat caaccggacg aggagccttt ggactaccat
    20581 tgtcgccaga attgttagaa gcgcctggtt cttcccagcc cgctggcgga cgtacatcgc
    20641 gacgtgccat caggtcatca atctgcggtg cgtcgatagt ctcatatttc atgagagcat
    20701 ctttcatcgc atgcagaata tccatattgt cggtcagaag ctgacgcgca cgattatagt
    20761 tacgctcaat cagtgctttc acttcctggt cgatgatacg tgcagtttca tcggacatat
    20821 gtttcgcttt cgctacgcta cggccgagga atacttcacc ttcttcttcc gcgtacagca
    20881 gtggacccaa tttctcagag aagccccact gggtcaccat gttacgtgcc agattggtcg
    20941 caactttaat atcgttggac gcaccggtag aaacatgttc cggcccatag atgatctctt
    21001 ctgccagacg accaccgtac agcgtagaaa tctggctttc cagtttctga cggctggcgc
    21061 tgattgcgtc gccttcaggc aagaagaaag tcacacccag cgcacgaccg cgtgggataa
    21121 tcgtcacttt gtgcaccgga tcgtgttccg gcaccaggcg accgataatc gcatgacccg
    21181 cttcgtggta agccgttgat tctttctgcg cttccgtcat caccatggag cgacgttccg
    21241 cacccatcat gattttgtct ttcgctttct cgaactcaac catcgacaca acgcgtttgt
    21301 tgccacgagc agcgaacagt gccgcttcgt tcaccaggtt cgccaggtca gcaccggaga
    21361 aaccaggagt accacgggca atgattgccg cgtcgatatc gggggccaat ggtacgcgac
    21421 gcatgtgaac tttcaggatc tgctcacgac cgcgaacatc tggcaagccg accacaacct
    21481 gacggtcgaa acggccagga cgcagcaggg ccgggtcgag aacgtccgga cggttagtcg
    21541 cggcgataac gatgatacct tcgttacctt cgaagccatc catctcaacc agcatctggt
    21601 tcagagtctg ttcacgttca tcgtgaccac cgcccagacc agcgccacgc tggcggccta
    21661 cggcgtcgat ttcatcgata aagatgatgc acggtgccgc tttcttcgcc tgttcgaaca
    21721 tgtcacgaac acgggatgca cccacaccga cgaacatttc tacgaagtca gaaccggaga
    21781 tagtaaagaa cggaactttc gcttcgcctg caatcgcttt cgccagcaac gttttaccgg
    21841 tacccggagg accgaccatc aggacgcctt tcgggatctt accgccgagt ttctggaagc
    21901 ggctcggctc gcgcagatac tctaccagtt cagcaacttc ttcttttgct tcgtcgcagc
    21961 ccgcaacgtc agcaaaggtc gttttgatct gatcttccgt cagcatgcgc gctttgctct
    22021 taccaaacga catggcacct ttgccaccgc cgccctgcat ttgacgcatg aagaagatcc
    22081 agacaccaat cagcaacagc atcgggaacc aggagatgaa gatagaagcc agcaggcttg
    22141 gttcttcagg cggttcaccg acaaccttga cgttcttggt caacaggtta tccagtaatt
    22201 tcggatcctg aaccggaatg taagtggtat aacggttact atctttcttg gtaacgttga
    22261 tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt attgacctct tgtaggaagg
    22321 tagagtaatc caccttacgg ccattagact cgctgggccc aaagctctgg aatactgaca
    22381 tcagcacaac ggcaatgacc agccagagta ttaggttttt cgccatgtca ctcaagggat
    22441 taacctctta ttacaactgt gttaaaaaca gcgtcaggat actctatatc cagcatcttt
    22501 caaactttcg tctgaaatct cccggttagg gtttacgccc ggtcgctaca atatacactt
    22561 cccgcgaacg tgcacgagaa gagtccggct tacgaacttt gaccttcgta aacagggagc
    22621 gaatttccct tagatactca tcgaaacctt cgccctggaa caccttcact acaaaactgc
    22681 cacctggcgc taatacatca cgacacattt ctagcgccag ttccaccaga tacatggcac
    22741 gggggatatc caccgccggt gttccgctca tgtttggtgc catatcggac atgacaacct
    22801 ggactttgct gtcgccaacg cgctccagca gtgctttcat caccagttca tcacgaaaat
    22861 cgccctgaag aaagtccaca ccaacgatag gatccatagg taaaagatcg caagcgatga
    22921 tgcggccttt gccgccaatt tgggtgacca catattgtga ccaaccaccc ggagcagcac
    22981 caaggtcgac aaccgtcatt cccggtttaa agagtttgtc actttgctgt atttcatcaa
    23041 gtttaaacca ggcacgggaa cgtaacccct ttttctgtgc ctgttgaaca tatttatcgc
    23101 taaagtgttc ctgaagccag cggctggagc tggcagaacg cttcttacct gtcatttaac
    23161 tttcccatgg ggataactca tcgtaaccaa ttgcgtaaat ttttacgcgc ctatttggtg
    23221 atatatggga gatggcggta gaatgacccg ttttcaatcc caacgtaagc aaaaaatacg
    23281 atgaatctga gtactaaaca aaaacagcac ctgaaaggtc tggcacatcc gctcaagcca
    23341 gttgttctgc ttggcagtaa tggtttgacc gaaggggtgc tggccgagat tgaacaagcg
    23401 ttagagcacc atgaactcat caaggtgaaa atcgccaccg aagatcgcga aactaaaacc
    23461 ttgatcgtgg aagctatcgt gcgcgaaacc ggcgcctgta atgtacaggt catcggtaaa
    23521 acgctggtgc tttatcgccc aactaaagaa cgtaaaatct cgctgccacg ctaagattat
    23581 cctaaagtta cacacatttg ctgtgtaaaa cgaggggttt tccgcaggca ggagagcaaa
    23641 atgccacgct ctgttcgttg ataaaaggcc gcatagcggc ctttttcctt tctttacaat
    23701 acatcaacat cttgagtatt gggtaattct tacaggtatt ccaccttaat tacttcaaat
    23761 tctacttcgc cgcccggcgt tttgatgacc acaacatcat cttcttcttt gccgatcagg
    23821 ccacgagcaa taggcgagtt tacagaaatc aggttttgtt taaagtcagc ttcgtcatcg
    23881 ccaacgatgc gataagtctg ttcttcgtca gaatccagat tcagcaccgt tacggtagca
    23941 ccgaaaataa cgcgcccatt gttgggcatt ttggtgacat caatcacctg cgcgttcgac
    24001 agcttggctt cgatgtcttt aatacggcct tcgcagaaac cctgctgttc acgagctgcg
    24061 tggtattcgg cgttttcttt caggtcgcca tgctcacgcg cttccgcgat agcagcaatg
    24121 atttcaggac ggcgcacaga tttcagaaaa tccagctctt cgcgtaattt ttcagcgccg
    24181 cgtaaggtca tcggaatagc ttgcatttgt tatacctctt gaatattcct gatggggcaa
    24241 gtcttcaccc accccggctg ttcggcctgc ccggcataac tcctcaccgg gaccagaagc
    24301 aaaaaaatac cgacccgggt acaagtccca ggtcagctac aattcacatt ttgatagtca
    24361 ttttaccctg aagttcccga agggtcatcg tttactttat agggcgttgc gccgtagtat
    24421 gacggctcga ttccaggttg ttagcgcgag attatgcgat tttccagatt tatcatcgga
    24481 ttgaccagct gtatagcgtt cagtgttcag gccgcgaatg ttgatgagta cgttactcaa
    24541 ctccccgctg gtgccaacct tgccctgatg gtgcaaaaag tcggcgcgtc ggcccccgct
    24601 attgattacc acagtcagca gatggcgctg cccgccagta cgcagaaagt gattactgcg
    24661 ctggcggcgt tgattcaact cggccccgat tttcgtttca ccacgacgct tgaaaccaaa
    24721 ggcaatgtgg aaaacggcgt acttaagggt gacttagtgg cgcgatttgg tgccgatccg
    24781 acgttaaaac gtcaggatat tcgcaatatg gtcgcgactt tgaaaaaatc tggcgtcaac
    24841 caaatcgatg gcaatgtgtt gatagatacc tccattttcg ccagccacga taaagccccc
    24901 ggctggccat ggaatgacat gacacaatgc tttagcgctc cgcctgccgc tgccatagtt
    24961 gaccgcaact gtttctccgt ctcgctctac agtgccccaa aacctggtga tatggctttt
    25021 atacgcgtgg catcttatta ccccgttacg atgttcagcc aggtacgcac cctcccccga
    25081 ggttctgccg aagcgcaata ctgcgaactg gatgtcgtac caggcgacct gaaccgcttt
    25141 acgctgacgg gatgcctgcc acaacgttct gagccgctcc cgttggcttt tgccgtgcag
    25201 gatggagcca gctatgccgg ggcaattctg aaagatgagt taaaacaggc aggtatcacc
    25261 tggagcggaa cactgctgcg ccagactcag gttaacgaac ctggaacggt agttgccagt
    25321 aaacagtcgg ccccgttgca cgatctgctt aagattatgc tgaaaaagtc ggacaacatg
    25381 atcgccgata cggttttccg catgataggc catgcgcgct tcaatgtgcc tggaacatgg
    25441 cgggccgggt cggacgccgt gcgtcagatc ctgcgccagc aagccggtgt cgatattgga
    25501 aacaccatta ttgccgatgg ttcagggctt tcgcggcata acctgattgc ccccgccacc
    25561 atgatgcagg tgctgcaata cattgcccaa cacgacaatg aacttaactt tatctccatg
    25621 ctgccgcttg cgggctatga cggctctttg cagtaccgtg caggtctgca tcaggcgggc
    25681 gtggatggaa aagtctcagc gaaaaccggt tcgttgcagg gggtatataa cctggcggga
    25741 ttcattacca cagcgagcgg gcaacgaatg gcgtttgtac aatatctttc tggctatgca
    25801 gtagaaccgg cggatcagcg taatcgccgt attccgttag tgcgttttga aagccgtttg
    25861 tataaagata tttatcagaa caattagtca aaaagaaacc ccggcacatg gccggggttt
    25921 tgctgattaa cgcttgtaaa tgaactcaac gccttcttcg tcgtcttcgt cccagtcatc
    25981 atcccagtcc tcatcatcct cttcagcaat ctcttcaagc tgctggcgat gataatcatc
    26041 ccacatgaat tcgactttct ctggctgttt cgcttcttca gcctgcacga ccgggttttc
    26101 aatgataaag gtcatcacat cccagcagag atctttcacg cccagtccac tcgccgcaga
    26161 gatcagataa tatttatctt cccagcccag cgcttcagca atcgctttcg ctttctcttc
    26221 agcttctgcc ttatccagca gatcgatctt attgaacact aaccaacgcg gtttcgccgc
    26281 cagatcctgg ctgtattttt ccagctcgct gataataata cgcgcgtttt caaccggatc
    26341 ggtgccgtca atcggatcga tatcgatgag gtgcaacagg acgcggcaac gttccaggtg
    26401 cttcaggaag cggataccca gacctgcgcc ttccgcagcg ccttcaatca gtcctggaat
    26461 atcggcaaca acgaagctct tttcgttgtc cattcgtacc acacccagac ttggcaccag
    26521 agtggtaaac ggataatccg ccactttcgg tttagccgcc gataccgcgc gaataaaggt
    26581 cgatttaccc gcgtttggca tccccaacat accgacgtca gccagcagca tcagctccag
    26641 cagcagctca cgcttatcac ccggcgtacc gttggttttc tgccgtggtg tacggttaac
    26701 ggacgatttg aaacgggtat tgcccagacc gtgccagccg cccttagcaa ccagcagacg
    26761 ctgaccgtgt ttagtcatat cgcccatggt ttcaccagta ccttggtcga ttatacgcgt
    26821 acctaccggc actttaatcg tcacgtcttt accgcgctta ccggtacaat cgcggcttgc
    26881 gccgttctga ccgcgctctg cacggaaaga tttttcaaaa cgataatcga taagcgtgtt
    26941 caggttttcg tcggcttcca tccatacgtc accaccatca ccaccgtcgc cgccatccgg
    27001 gccgcctttc ggaatatact tttcgcggcg gaagctcacg caaccattac cgccatcacc
    27061 tgcaacgacc agaatcgatg cttcatcaac aaacttcatt ttattctccg taaatcattc
    27121 gcctgcgcgg ggttgcgata ccaccgttgt atgcttacgt aatccgcccc aaatacgatg
    27181 accaatggcg gaatacatcg cgcccgcaac cacgacaaac gcaccgagat aacctaaaag
    27241 gtttaacatc ggtctggcga agaaatcggg ccaggccagt gataaaagat ctgaaaaaaa
    27301 cagcgtaaac agtggggtga gcgtgatgat cgcgctcacc tgcgctgcct gccagcgagc
    27361 catcgcttcc gccagggcgc catatcctac caatgtattc agtccgcaaa aaattaaaca
    27421 tgcgagctgc cagtggctaa gctgcgctat cactccaggc tttgccagag ggaagagcgc
    27481 aattgtacat aaagcgtaca gtaaaaacag gatctgcggt gaggccagcc gacgcaataa
    27541 aaccttttgc gccacgccat aactcaccca aaccgtcgcc gcaccgaccc caaagataac
    27601 tccccaggtg taatcggtga gctttgtaaa tatctcgacc agactggtgt taaaaaacat
    27661 caccaggccg ctcaggagca tcaatgcccc tacaacctga gtgctgcgca ttttctcttt
    27721 caggataaat acgctggcaa ccatcatgcc aactggcgag agttgcccaa tcacctgcga
    27781 agcggtcgga ctcaggtatt gcaaggatga gctgaacagg atgaagttcc caaacagccc
    27841 ggcggtcgcc actgccaaca aaatcaacca gcgtggctta cgaaacacgc gtaatggcgg
    27901 caacctcttc ttcaccgcaa gaatggcacc aaggccaata ctcgccatca agaaacggta
    27961 aaacacgatt gtcggaggtt ccatcacctc cagcacctgc ttcattgcga ttggcaacgc
    28021 cccccagcaa attgctgtgg tgagcgccaa aagaatgcca atgcctgcct gctgcttcat
    28081 accgtattcc ctgcaaagcg catttgcgga ttaacgtcgc accatgcgca tttttaccgg
    28141 ttatcgaatg taaaaagccc cgcaacgtgt tgcggggctt tcatccgtta ccgggacgcg
    28201 aaaaacttat tcagcttcga tgctgataaa tttacggttt ttcgggcctt taacttcgaa
    28261 tttcacttta ccgtctgctt tagcaaacag agtgtggtca cgaccgcaac ctacgttagc
    28321 gccagcgtgg aatttggtac cacgttgacg aacgatgatg ctacccgcca gaacggattc
    28381 gccaccgaaa cgcttaacgc ccaggcgttt agcttctgaa tcgcgaccgt tacgtgtgga
    28441 gccgccagcc tttttatgtg ccatttgaaa tctctcctca ggtcttaggc gctgatgcca
    28501 gtaattttca catcagtgaa ccactgacga tggccctgct gcttacgata gtgtttacga
    28561 cgacgaaact taacgatttt aactttctcg ccacgaccgt gagcaacaac ttcagctttg
    28621 attacgccgc catcaacgaa aggaacgccg attttgactt cttcaccgtt tgcgatcatc
    28681 agcacttcag cgaactcaac agtttcgcca gttgcgatgt ccagcttttc caggcgaacg
    28741 gtctgacctt cgcttactcg gtgttgttta ccaccacttt ggaaaaccgc gtacatataa
    28801 aactccgctt ccgcgcacac cttttcaatg attcagagtg cgctataaat attcacaata
    28861 gggcgcgaat attacgcaaa acgcacgcct ttgacaagtg ctacagtcaa tacacgaaga
    28921 aaaaaaacac aacttgtacg gtaacgttta tctgtgccat tttttcagta caatcactct
    28981 atattcctaa ccataaaccc taagttgcct ttgttcacag taaggtaatc ggggcgaaaa
    29041 gcccggcttt tgcgatgaat ttagaaaaaa tcaatgagtt aaccgcgcaa gatatggcgg
    29101 gtgttaatgc ggcaatcctt gagcagctta attccgacgt ccagctgatc aatcagttag
    29161 gctattacat cgtcagcggc ggcggtaaac gtattcgtcc gatgattgct gtactggctg
    29221 cacgagctgt tggctatgag ggaaatgcgc atgtcaccat tgctgccctg atcgagttta
    29281 tccacacggc gactctgctg cacgacgacg ttgtggatga atcagatatg cgcaggggta
    29341 aagctaccgc caacgctgca tttggcaatg ccgccagcgt gctggtaggc gattttattt
    29401 atacccgcgc gttccagatg atgaccagcc tcggttcgct caaagtgctg gaagtcatgt
    29461 cagaagccgt aaacgtcatc gcagaaggtg aagttctgca actgatgaac gttaacgatc
    29521 cggacatcac tgaagaaaac tacatgcgcg ttatctatag caaaaccgcg cgtctgtttg
    29581 aggctgccgc gcagtgttcc gggattctgg ctggctgtac gccggaagag gagaaaggcc
    29641 tgcaggatta tgggcgctat ctcggcactg ctttccagtt gatcgacgat ttactcgatt
    29701 acaatgccga tggcgaacag ttaggtaaaa atgtcggcga cgatctgaac gaaggtaaac
    29761 cgacgctgcc gctgctgcat gcgatgcatc atggcacacc agaacaggca cagatgatcc
    29821 gtaccgccat cgaacagggt aacggtcgcc atcttctgga accggttctg gaagcaatga
    29881 acgcttgtgg atctcttgaa tggacgcgtc agcgtgccga ggaagaagca gacaaagcca
    29941 tcgcagcgtt acaggtgctc ccggacaccc cttggcgaga agcactcatc ggcctcgcgc
    30001 acatcgctgt tcaacgcgat cgttaatccc ctcccctcat cccgcgcagc gcgcggggtg
    30061 gttccagtaa attccataaa aacacttact cagctctata acacctgaaa aacaggctgt
    30121 gcatctttat atattctgaa tattcacact ccttacagga actttttaga gcaatcggcc
    30181 atcaggagta tagtgatgct cgacagaaga agtgttctga atgaaagcga ctaacttaag
    30241 gagtgaggaa agatggaaag taatttcatt gactggcatc ccgctgacat cattgcgggt
    30301 ttgcgcaaga agggaacgtc aatggcggcg gagtctcgca gaaatggttt gagttcctca
    30361 acgctggcga atgcattatc gcgcccatgg ccgaaaggag agatgattat tgcgaaagcc
    30421 ctgggaactg acccctgggt tatctggcca tcacgctacc atgatccgca gacccatgag
    30481 tttatcgaca gaacgcagtt gatgcgtagc tacactaaac cgaaaaaatg agtggtctgg
    30541 cggtagcccc gcgaacgggg ctgccagctc tcagacgatt actcgccttt cacacgctca
    30601 atatttgcac ctaaagcgcg cagtttgtct tcaatgcgtt cgtagccacg atcgatgtga
    30661 taaatacgat caaccaccgt cgtcccttcc gcaatacagc cagccagcac caggcttgct
    30721 gatgcacgca gatcggttgc cataacctgt gcgccagaaa gtttttcaac accgtgacaa
    30781 ataacggtat tgctttcgat ttcggcatgc gcgcccatac ggctaagctc cggcacatgc
    30841 ataaagcggt tttcaaagac cgtttcggtg atgaatccgg tcccttctgc caccaggttc
    30901 aacagcgtga actgggcctg catatcggtc gggaatgccg gatgcggcgc ggtacgtacg
    30961 ttaacagcct tcggacgttt gccatgcata tccaggctaa tccagtcttc gccgacttcg
    31021 atgtccgctc cagcgtcacg cagtttcgcc agcacggcgt cgagagtatc tggctgcgcg
    31081 ttacggcaga taattttgcc gcgagaaatc gccgccgcca ccaggaaagt accggtttcg
    31141 atacgatccg gtagcacgcg atagacaccg ccgcctaaac gttccacacc ttcgatgacg
    31201 atacgatcgg tgccctgacc gctaattttc gcacccagcg taatcaggaa gttcgcggta
    31261 tcgacgattt ccggttcacg cgctgcgttt tcaataatcg tggtaccttc cgccagggtt
    31321 gcagcacaca tgatggtcac cgttgcgcca acgctgactt tatccatcac gatatgtgcg
    31381 cctttcagac gaccatcgac ggaagcttta acgtaacctt cttccagttt gatggtcgcg
    31441 cctaattgtt cgaggccaga aatgtgtaga tcaaccggac gcgcaccgat cgtacaaccg
    31501 ccaggcagtg aaacttgccc ctgaccaaag cgcgctacca gcggccccag tgcccagata
    31561 gaagcacgca tggttttaac cagatcgtaa ggtgcgcaga atacattaac gtcgcgggca
    31621 tcaatatgca cagaaccatt acgttctact ttcgcaccca gctggcttag cagcttcatt
    31681 gatgtatcga cgtctttcag tttcgggacg ttctggatct ctaccggttc ttccgccagt
    31741 agagcggcaa aaaggatagg cagagcagca tttttagcgc cggaaattgt gacttcgccc
    31801 tggagcttcg ttggcccctg aacacgaaat ttatccattt agtttgttct cagttaacaa
    31861 ttcatatccg ctaccggcga atcgcccata gctcaaaagc cgttcagttt gcgatcgcgc
    31921 gcccactccg caggggtata cgctttgatc gacacagcat gaatgcggtt atccgcaata
    31981 tattccatca gcgggccata gaccgtctgc tgttttttaa cccgactcat gccgtcaaac
    32041 aactcaccca cggcaataac ctgaaagtgg ctgccatcgc cggaaacgtg gacttcctgg
    32101 agggagagag cgttcatcaa cacgctctga atttcattat tttccatggg atcttcaatc
    32161 atcagttaat aaaccagcga aacatcttag agcaaagttg cgctggcata aataagcaaa
    32221 aagcctcgct gataaatcag acaaggctcg acttgcaggc aggtttgccg gacaggcggt
    32281 taacgccata tccggcctga aaaaatttaa cgaggcagaa catcagcagg caaattatac
    32341 aatttcgcga gggtatacac tttgtcgttt accccctgaa gcgtcacgtt gttgccctgc
    32401 tttttcgcca gatcgataag atggagcagc agtgccagtc cacccgtatc cacgcgggac
    32461 acacggctaa gatcgatgca ggtaatcccc ttcaccgctt cctcacgcat ttcccaaagc
    32521 ggtagcagaa cgtcctgatc cagctctccg gataacgcca gcgtgtcacc cgtctgcatc
    32581 cagctcagtg actcgctcat tattttttct cttccagagt gattttctgt tgagaaatcg
    32641 atttcagttg cgcagtcagg ccgtcgatac ctttggtacg cagcagcgtt ccccactcgt
    32701 tttgtttggt ggtgatcata ctgacgcctt cagcaatcat gtcgtaagcc tgccagtttc
    32761 ccgtctggga gtttttacgc cactggaagt ccagacgcac cggcggacgg ccattcgggt
    32821 caataatggt aacgcgaata ggcacaatgg ttttatcgcc cagcggctgt tctggcgcaa
    32881 tctgataggt ttgaccgtga tacatcgcca gcgcctgacc gtaagcctgc ttcaggtact
    32941 cacggaaagc ggcaaagtag gcttcacgtt gagcaggggt cgcactcttg taatactggc
    33001 ccagcaccag cgcaccggcg tatttcacct gtacgtatgg cagcagttcc tgatcaacaa
    33061 tggtacgcag ataatccggg ttggcccgaa tttgcggttg ctcattcttc aggcgatcga
    33121 acgttttctg cgccgcctcg tccatcagct tatacggatt ggtctggtct gccgcggttg
    33181 ccgcactcag aggtgcaatc accagcaaag cgaccatcat taaacgttta aacatgcgtc
    33241 ggttctcctg aaattatttc gttgtaccca caggttcagt ggtttcatta ttacctggcg
    33301 cagcagctgg cgcatcgcca ctattcttat tgtcatcgcc tttactaccg taaaggaact
    33361 gaccaatgag atcttccagc accatcgcag acttagtgtc ctgaattgta tcgccatcct
    33421 tcaggatagc agtccccagt tccgggtctt caaaaccgac gtttaatgcc agatattgtt
    33481 cccccagcag gccggaagta cgaatgctca gcgaactggt atctggaatg tggttataac
    33541 gttgttcaat ttccagcgtt acgcgtggca gataggtttt cgggtccagc gtaatatccg
    33601 ccacccggcc cacaacaacg ccaccaatac tgaccggaga gcgcgctttc aggccgccaa
    33661 tgttatcgaa cgtcgcataa agcgtgtagg tcggttcagt acgtatggac gtcacgttcg
    33721 ccgccttcag gcaaacaaac agcgccgcca gcagtgctgc taataaaaag atacccaccc
    33781 aaatttcatt ttttttcgtt tgcatgaact caattcccaa acatcaatgc ggtcagcaca
    33841 aaatccagcc ccagaacagc cagagacgag tggacaacgg tgcgagtggt tgcccggcta
    33901 atcccggcag acgtcgggat ggcgtcgtag ccgttaaaca acgaaatcca cgtcaccgtg
    33961 atggcgaaca ccacgctctt aatcagacag ttgaccagat ccatacgcca gtcgacggca
    34021 ttttgcattg ccgaccagaa gaacccgcta tcaatgcctt tccaactgac gccgaccaga
    34081 gatccgcccc agatccccac ggcgacgaaa ataaccgtca acagtggtaa tgaaataacc
    34141 ccagcccaga aacggggaga aataacccga cgcagcggat ccaccgccat catctccata
    34201 ctggagagtt gctctgtagc gcgcatcagg ccgatttctg cggttagcgc cgaaccagca
    34261 cgcccggcaa acaacaacgc ggcaaccacc ggccccagtt cacgcagtag cgataacgcc
    34321 accagcatac ccagactggt ttccgcacta taagtggtca gaaccagata accttgcagc
    34381 cccaacacca ttccgatgaa cacgccagaa accacaataa tcagcatcga caggacgccg
    34441 acattataga gctggcgcac cagcagcggc gcatgtttgc gaaattccgg tttgccgacc
    34501 agcgcattga ataacattaa cccggcccgc ccgaacgttc tcagggtttt aatcccttta
    34561 tgtccgagcg acgccagcgc atttaacagc atgagtggct taactccctg gtaaaagatc
    34621 agcgtgataa tcgccggcag gatagcggaa cggaacaggc ccgtcagcta tcccgtccag
    34681 aaactgacgt acgcgcggat caggattcgc ctgcaacgcc tgggcactgc catgagcgac
    34741 aatttttttg tccgccagga tccaggcgtg atccgcaata cttaacactt ccggcacatc
    34801 gtgagaaacc accacacaag tcacgcccag cgcgctgttc agctcagaaa tcagcttcac
    34861 cagtacgccc atggtgatgg gatcttgccc aacaaaaggt tcatcaaaca tgatgagatc
    34921 cggctccagc gcaatcgcac gcgccagcgc tgcacgccgc gccatcccac cagaaagttc
    34981 agaaggcatt agcttagccg ctccacgcag ccccacggcc tccagcttca tcatcactgt
    35041 actatgcaac aatggcgcgg gaagttgggt atgttcgcgc agtggatagg cgacgttgtc
    35101 aaatacgttc atatcagtga acaacgcccc ggactgaaat aacatgctca tccgtttgcg
    35161 cactgtatac aggcgcgaac gagacatcgc cggaatattc tcaccatcga aaaggatctc
    35221 accatgatct ggtgcgattt gcccgccaat cagacggagt agcgtcgttt taccgatgcc
    35281 cgatggcccc atgattgccg tgatcttccc tcgcggcacg gtcagggaaa tattatcgaa
    35341 gatgcagcga ttgccacgcg taaaactgac atcgcgcata tcgactaaat tcgccacaga
    35401 ctgctccata attcaccctt cgtcttgcgt tgattttcta agcatggcgc tcaatttaac
    35461 cttgaaccca acatatttac agaatattac ccgccgtggt tagcgaaagc tggcatttgt
    35521 tttacttttt agccgcataa agtcaaaatt aagaatccgt tacggctttc tgaaaatctt
    35581 cagcggaccg gcgagtatac ctgaagaaag gatgttagat gcttttagct acggcactgt
    35641 taattgttgg tttacttttg gtcgtttaca gtgccgaccg cctggttttt gccgcgtcta
    35701 ttctttgccg aacctttggc atcccgccgc tgatcatcgg catgacggtg gtcagtattg
    35761 gtacatcgtt accagaagtc atcgtctcgc ttgccgcgtc tctgcacgaa caacgcgatt
    35821 tagccgttgg tacagccctc ggctcaaaca ttatcaatat attgctgatc ctcggtctgg
    35881 ctgcgctggt tcgtcctttt accgtccatt ctgatgttct acgccgtgaa ttacccttaa
    35941 tgttgttggt cagcgtggtg gccggttccg tactctatga cggacaactt agtcgcagcg
    36001 atggtatctt tctcctcttt ctggctgtgc tatggctgct gttcattgtt aaacttgcac
    36061 gtcaggctga acgtcagggg actgacagcc tgaccagaga gcagcttgca gagctgccgc
    36121 gtgacggcgg attgcccgtc gcgtttttat ggctcggcat tgcgcttatc atcatgccag
    36181 tggccacgcg gatggtggtt gataacgcca cggtgctggc gaattacttt gccatcagcg
    36241 agttgacgat gggtctgacg gcaattgcta tcggaaccag cctgccggaa ctggcaaccg
    36301 caatagcggg ggttcgcaaa ggtgaaaacg acattgctgt cggaaatatc attggcgcaa
    36361 acatttttaa tattgtcatc gtgttgggtt tacccgcgct gataacgcca ggagagattg
    36421 atccactggc gtacagtcgt gactacagcg tgatgttgct ggtgagcatt atttttgcgt
    36481 tgctgtgctg gcggcgctcc ccacaaccgg gccgtggtgt aggggtatta ttaactggcg
    36541 gatttatcgt atggctggcg atgttgtact ggttatcgcc aatactcgtt gaataactgg
    36601 aaacgcatta tgtcgcacgt agagttacaa ccgggttttg actttcagca agcaggtaaa
    36661 gaagtcctgg cgattgaacg tgaatgcctg gcggagcttg atcaatacat caatcagaat
    36721 ttcacgcttg cctgtgaaaa gatgttctgg tgtaaaggga aagttgtcgt catggggatg
    36781 gggaaatcgg ggcacatcgg gcgcaaaatg gccgcaacgt ttgccagcac cggtacacct
    36841 tcatttttcg tccatcctgg tgaagccgcg catggtgatt taggcatggt caccccacag
    36901 gatgtggtga ttgctatctc taactcaggt gaatccagcg aaatcacggc cttaattcca
    36961 gtgcttaagc gtcttcacgt accgttaatc tgcatcaccg gtcgcccgga gagcagcatg
    37021 gcgcgcgctg cagatgtgca tctgtgtgtt aaagtagcga aagaagcctg tccgttaggg
    37081 ctggcaccga ccagcagcac caccgccacg ctggttatgg gcgatgccct cgctgtcgcg
    37141 ctgttaaaag cacgcggctt tactgctgaa gattttgcgc tctcacaccc aggcggcgca
    37201 ctgggtcgta aacttctgct gcgcgtaaac gatattatgc atacgggcga tgagatcccg
    37261 catgttaaga aaacggccag tctgcgtgac gcattgctgg aagttacccg caaaaatctt
    37321 ggtatgactg tcatttgcga tgacaatatg atgattgaag gcatctttac cgacggtgat
    37381 ttacgccgtg tcttcgatat gggcgtggat gttcgtcagt taagtattgc cgatgtgatg
    37441 acgccggggg gaatacgtgt gcgccctggc attctggccg ttgaggcact gaacttaatg
    37501 cagtcccgcc atatcacctc cgtgatggtt gccgatggcg accatttact cggtgtgtta
    37561 catatgcatg atttactgcg tgcaggcgta gtgtaaagat tcaaggataa acaacaatga
    37621 gcaaagcagg tgcgtcgctt gcgacctgtt acggccctgt cagcgccgac gttatggcaa
    37681 aagcagagaa cattcgtctg ctgatcctcg acgttgatgg cgtactgtca gatggcctga
    37741 tttatatggg caataacggc gaagagctga aagcgttcaa tgttcgtgac ggttatggca
    37801 ttcgttgtgt gctcacctct gatattgaag tcgctatcat taccgggcga aaggctaaac
    37861 tggtagaaga tcgttgtgcc acattgggga tcactcactt gtatcagggg cagtcaaaca
    37921 aactgatcgc ctttagcgat ttgctggaaa aactggcgat tgccccggaa aatgtggctt
    37981 atgtcggcga tgatctcatc gactggccgg taatggaaaa agtgggttta agcgtcgccg
    38041 tggccgatgc gcatccactg ttgatcccgc gcgccgatta cgtgacgcgc attgctggcg
    38101 gtcgtggcgc agtgcgcgaa gtttgcgact tattactcct ggcgcagggc aaactggatg
    38161 aagccaaagg gcaatcgata tgagtaaagc cagacgttgg gttatcattg tgctatcact
    38221 ggcggttctg gtgatgatcg gcattaatat ggccgaaaaa gacgataccg cccaggtggt
    38281 cgtcaacaac aatgatccca cctataaaag cgagcatacg gacacgctcg tctataaccc
    38341 agaaggggca ctaagctatc gattgattgc tcaacacgtt gaatattatt ccgatcaggc
    38401 cgtttcgtgg tttacgcagc cggtacttac cacgtttgat aaggataaaa tcccgacatg
    38461 gtccgtaaaa gcagataaag ccaagctgac caatgaccgg atgctctatt tatatggaca
    38521 cgttgaagtc aacgcactcg tgccggactc tcaacttcgc agaatcacga cggataacgc
    38581 gcagatcaat ctggtgacgc aggatgttac ctctgaagac ctcgtcacgt tatacggaac
    38641 aacatttaac tccagcggtc tgaaaatgcg cggcaactta cgcagcaaga acgccgagct
    38701 gattgaaaag gttagaacat cctatgaaat tcaaaacaaa caaactcagc cttaatcttg
    38761 tgcttgccag ctcacttctg gccgccagca ttccggcatt tgccgtaacc ggagacactg
    38821 atcagccgat ccacattgaa tcggaccagc aatctcttga tatgcaaggc aacgtggtta
    38881 cctttaccgg taatgtcatc gtcacccagg gcaccatcaa aattaatgcc gacaaagtgg
    38941 ttgttacccg tccgggcggc gaacaaggta aagaagtgat tgacggctac ggtaaaccgg
    39001 caacgttcta ccagatgcag gacaacggta aaccggttga aggtcacgct tcccagatgc
    39061 actacgaact ggcaaaagat tttgtcgttc tgacgggtaa tgcttatctg cagcaggttg
    39121 atagcaacat taagggcgat aagatcactt acctggtaaa agagcagaaa atgcaggctt
    39181 tcagcgacaa aggcaagcgc gtaaccaccg ttctggtgcc gtcgcagctg caggacaaaa
    39241 acaacaaagg ccagaccccg gcacagaaga agggtaatta attcgttatg gcaacattaa
    39301 ctgcaaagaa ccttgcaaaa gcctataaag gccgtcgcgt ggtagaagac gtcagcctga
    39361 ccgtcaactc cggggaaatt gtcggtctgc tggggccaaa cggtgccggt aagaccacca
    39421 ctttctatat ggttgtaggc attgtgccgc gcgatgcggg caacatcatt attgatgatg
    39481 acgatatcag tctgctgcct ctgcatgcac gcgcgcgccg cggtatcggc tatctgccac
    39541 aggaagcctc cattttccgt cgcctcagcg tttacgataa cctgatggcg gtactgcaaa
    39601 ttcgtgacga cttgtctgct gaacaacgtg aagaccgcgc gaacgagctg atggaagagt
    39661 ttcacattga gcacctgcgt gacagcatgg ggcagtcact ctccgggggt gaacgtcgcc
    39721 gtgtagaaat tgcccgcgca ctggctgcga atccgaaatt tattctgctc gacgaaccgt
    39781 ttgccggtgt tgacccgatc tcggttatcg acattaaacg catcattgag cacctgcgcg
    39841 acagcggcct gggcgtgctg atcactgacc acaacgtgcg tgaaacactg gcggtttgtg
    39901 aacgcgctta tatcgtcagt caggggcatt tgatcgccca cggcacgcct acagaaatct
    39961 tacaagacga acacgttaag cgtgtatacc ttggggaaga cttcagactc tgatagggta
    40021 gaagtttgcg acgttttagc aggagagtac gattctgaac atgaagcaag gtttgcaact
    40081 caggcttagc caacaactgg cgatgacgcc acagctccaa caggcaattc gtctgttgca
    40141 gttgtcgacg ctggaacttc agcaggagct acagcaggcg ctggagagta atccgctgct
    40201 tgagcaaatc gacactcatg aagaaatcga cacccgcgaa acgcaagaca gtgaaacgct
    40261 ggacaccgcc gatgcgctcg aacaaaaaga gatgccggaa gagctgccgc tcgatgccag
    40321 ttgggacacc atttacaccg ctggtacacc atccggcacc agcggtgact acattgacga
    40381 tgagctgccg gtctatcagg gcgaaacgac gcagaccttg caggattacc tgatgtggca
    40441 ggtcgagctg acaccgtttt ccgacactga ccgcgctatt gctacctcta tcgtcgatgc
    40501 cgttgatgac accggttatc tgactgtccc gctggaagat attctcgaaa gtatgggcga
    40561 tgaagagatc gacatcgacg aggttgaagc cgtccttaag cggatccaac ggtttgatcc
    40621 ggtcggtgta gcggcaaaag atctgcgtga ctgcctgcta atccaactct cccaattcga
    40681 taaaaccacg ccgtggctgg aagaggccag attgatcatt agcgatcatc tcgatctgtt
    40741 agccaatcac gacttccgca ctttaatgcg cgtcacgcgt ctgaaagaag atgtgctgaa
    40801 agaagccgtc aatctgatcc agtcgctcga tccgcgcccc gggcagtcga tccagactgg
    40861 cgaacctgag tatgtcattc cagatgtgct ggtgcgtaag cataacggtc actggacagt
    40921 agaactcaac agtgacagca ttccgcgtct gcaaatcaac cagcactacg cctcgatgtg
    40981 caataacgct cgcaatgatg gtgacagcca gtttatccgc agcaatctgc aggatgccaa
    41041 atggttgatc aagagtctgg aaagccgtaa cgatacgcta ctgcgcgtga gtcgctgtat
    41101 cgttgaacag cagcaagcct tctttgagca aggcgaagaa tatatgaaac cgatggtact
    41161 ggccgatatc gcccaggctg tcgaaatgca tgaatcgacg atatctcgcg tgaccacgca
    41221 aaaatacctg catagtccac gaggcatttt tgaactgaag tatttctttt ccagtcacgt
    41281 caataccgag ggcggcggcg aagcttcctc cacggcgatt cgtgcgctgg tgaagaaatt
    41341 aatcgcggcg gaaaacccag cgaaaccgtt gagcgacagc aagttaacct ctttgctgtc
    41401 ggaacaaggt atcatggtgg cacgccgcac tgttgcgaag taccgagagt ctttatccat
    41461 tccgccgtca aaccagcgta aacagctcgt ttgacccaac cgataaggaa gacactatgc
    41521 agctcaacat taccggaaat aacgtcgaga tcaccgaggc actgcgcgaa tttgttacag
    41581 ccaaatttgc caaacttgag caatattttg accgaatcaa ccaggtctat gttgttctga
    41641 aagtggagaa agtcacccac acctcagatg caacactgca tgtaaacggt ggtgaaattc
    41701 atgccagcgc ggaaggtcag gacatgtacg ctgccattga tggcttaatt gataagctgg
    41761 cacgccagtt gaccaaacat aaagataaac tgaaacaaca ctaattgtcc gggcaattag
    41821 catgtgcatg gcggcctgtt gtgcggcaca acgggccatt tgtacggtta atgctccgag
    41881 cctgttccac tgtttgagtg ggcaggttct taggtgaaat tatgacaaat aatgatacaa
    41941 ctctacagct tagcagtgtt cttaacaggg aatgtacgcg aagccgcgtc cactgtcaga
    42001 gcaaaaaacg cgccctggaa atcatcagcg agctggcggc gaaacaactt agcctgccgc
    42061 ctcaggtggt ttttgaagct atcctgacgc gggaaaaaat gggcagtacc ggtatcggca
    42121 atggtattgc cattccgcat ggcaaactgg aagaagatac tctgcgcgcc gttggcgttt
    42181 tcgttcagct cgaaacgcct atagctttcg atgccatcga caaccaaccg gtggacttac
    42241 tttttgccct gctggtgccg gcagaccaaa ctaaaacgca cctgcatact ctgtcgctgg
    42301 tggcgaaacg tctggcggac aaaaccatct gccgccgttt acgcgcagcc cagagcgatg
    42361 aagagctgta tcaaatcatt acggataccg aaggtactcc ggatgaagcg tagttattcg
    42421 gtaatgtctc ttttagacgt tgtgaggaga aacagtacat ggtactgatg atcgtcagcg
    42481 gacgttcagg ttcaggtaaa tctgtcgccc tgcgtgcgct ggaagatatg ggtttttact
    42541 gcgtggataa ccttcccgta gtgttgttac ccgatctggc tcgaactctg gccgatcgcg
    42601 agatttctgc cgccgtcagc attgatgttc gtaatatgcc ggagtcacca gaaatattcg
    42661 aacaggcgat gagtaacctg cctgacgctt tctcaccgca actactgttc ctggatgccg
    42721 accgtaatac cttaattcgt cgttacagtg acacgcgccg actgcatccg ctttccagca
    42781 aaaacctgtc gctggaaagt gctatcgaca aagaaagcga tttgctggag cctctgcgtt
    42841 cgcgagcgga tctgattgtc gatacctcag aaatgtccgt tcacgagctg gctgaaatgc
    42901 tgcgtacccg tctgctgggt aaacgtgaac gcgaactgac catggtcttt gagtctttcg
    42961 gcttcaaaca cggtatccct atcgatgcag attacgtctt tgacgtgcgc ttcttgccga
    43021 acccgcactg ggatccgaaa ctgcgtccaa tgacaggtct tgataaacct gtcgccgcgt
    43081 tcctcgaccg ccacacagaa gtacacaatt ttatctacca gacgcgaagc tatcttgaac
    43141 tatggttacc tatgctggaa accaacaacc gtagctactt gacggtcgcc attggttgta
    43201 ccggcggaaa gcaccgttcg gtgtatattg cagagcaact ggcagactac ttccgctcgc
    43261 gcggtaaaaa cgtccagtca cgccatcgta cgctggaaaa acgtaaacca tgaccgtcaa
    43321 gcaaactgtt gaaatcacaa acaagctggg catgcatgcc cggcctgcaa tgaagctgtt
    43381 tgaattaatg cagggttttg acgctgaagt gctgttacgt aatgacgaag gcaccgaggc
    43441 cgaagccaac agcgttattg cgctgctgat gttggattct gccaaaggac ggcagattga
    43501 agttgaagcg accggcccac aggaagagga agcactggcc gccgttatcg ccctctttaa
    43561 ctctggtttt gatgaagatt aatcttcatc actttgacat acaactccct tcaaactccc
    43621 cccgacaata agaaaatcac gtactgaaat cgttctcaat caacgtcatt tgtacatttt
    43681 gtgcgctttt cactttcaga agaaccttaa gaaaacctta agagacattg tttaggtttt
    43741 gtttaagtta atcgaccata ctggagatcg tcagaaaata tttccaggag atggcatgat
    43801 tcgcttatct gaacaaagtc ccctgggcac cggaagacat cggaaatgtt atgcgcatcc
    43861 ggaagatgcc caacgctgta tcaagattgt ctaccatcgt ggcgatggtg gcgataaaga
    43921 gatccgccgt gagttaaagt actacgcgca tcttggtcgc cggttaaaag actggagtgg
    43981 aataccgcgc tatcacggta ccgttgagac ggattgcgga accggttatg tctacgatgt
    44041 gattgcagat tttgacggca aaccctccat tacgctgacc gaatttgctg aacaatgccg
    44101 ttacgaagaa gacattgcac aactgcgcca gctactgaaa cagctgaagc gttatttgca
    44161 ggataaccgt atcgtgacga tgtcgctgaa gccgcagaat atcctctgtc accgcatcag
    44221 tgagtcagag gttatcccgg tcgtctgtga caacattggt gaaagcacat tgattccact
    44281 ggcgacctgg tcgaagtggt gttgtctgcg taagcaagag cggttatgga aacgatttat
    44341 cgcccaaccg gcgctggcta ttgccctgca gaaagatctg caacctcgtg agagcaaaac
    44401 gctggccctc acttcgcgcg aagcttaatc cagctggtgt tgttgcataa acggctcacc
    44461 gcctaactga tacatctgcc gtaaaatcca cgcctgacgg ctacgcacgt agcccgatgg
    44521 cgaggagact ttgaaacgaa gtggattagg taatacagct gccagtaatg cagcttccga
    44581 ccgggtaagt ttgctcgcgg gtttgtggaa ataacgttgt gccgcagctt cgacgccaaa
    44641 cacaccgtcg ccaaattcgg cgatattcag gtaaaccgtc aggatacgct ttttgctcca
    44701 gactgtttct atccccagcg ttaatccagc ttccagccct tttcgtaccc agctacgccc
    44761 atcccataaa aagagatttt tggccgtctg ctgagaaatc gttgaagcac cgcgaatacg
    44821 gttttcattg cgctcgttgt gcgccagggc tttctcaatg gaagcgacat caaagcccca
    44881 gtgctcagga aatttctgat cttctgcggc aatcaccgcc agtcccatcc acggcgagat
    44941 ttgatccata ctgacccagt cagaatgtgc cacgtaacga aaattgccat gcagccaggc
    45001 gctgacctgt cgctcgacca ttaccgctga gaagggaaca ggcgcaacac taaacaacgc
    45061 gatgcccccg ccccagaaaa cggcgaggac aaccattaac cgcaaaagaa aacggcgaac
    45121 gaaactaaac accgttaagc ggcttttact cattcagcca gaaccagcac gcgggaaacc
    45181 agcttatcaa tgccgctcgc cgcttctgca atgttctgcg ccagcatata tgctggggtg
    45241 gtgacaatct tattgtcttc atcaaccacg atatcatcga caggacaggg cacatgctcc
    45301 gcgcccatct cttccagcac ttctgcggta tcgatatcag taccgatggt caaacgcagc
    45361 gggaaatcga aaattttcgg cagcatcgcc ggggcaatac acataaaacc aagcggtttt
    45421 ccggcctgat gcatcgcttg tgccagcgcc tttaattcac ggtcaacggt gcattcgcta
    45481 ccaagagtgg caaaattgct taaattcttc gccgcgccaa acccccccgg cacaatcaac
    45541 gcatccagtt cagcggcatc ggcctgggcc agaggacgga tttcaccgcg cgttattcgt
    45601 gccgcttcaa tcagcacgtt gcgcgtttcc gtcatcgctt cgccagttaa atggttgata
    45661 acatcaacct gctgcttatc cggtgcaaag cagaccgcct gcgcaccgct gcgtgaaata
    45721 gctaacaacg tcaacaccgc ttcatgaatt tcagaaccgt catagacgcc gcatccgctc
    45781 agaattacgc caattttctt cattgtgatc atcctttcgc aattgactga aacacattaa
    45841 tttttttaat aaaaatggta cgcatcacac atttaactga ttcatgtaac aaatcattta
    45901 agttttgcta tcttaactgc gtgcggcctg aaaaacagtg ctgtgccctt gtaactcatc
    45961 ataataattt acggcgcagc caagatttcc ctggtgttgg cgcagtattc gcgcaccccg
    46021 gtctagccgg ggtcattttt tagtggcttt tgccacccac gctttcagca cttctacgtc
    46081 gtgacgccac tcttctttca tctcttcaat ccattcaccg acgttatctt cccaggcagg
    46141 aaggtccgga gactgaattt gctgacccag ctgttgcaga tggcgtaacc ccactgaccc
    46201 cgccgcacct ttaattttat gtccttcctc tacaatgcct tttttatcct gcgccgtcag
    46261 attcgactcc agcacgctga catagcccgg catcatcttc tcaaacaccg ctaacccgtc
    46321 ggtgatcagc ttcggtccta caagttcgag atactgttcc agcatgggaa tatcgagcaa
    46381 tgcttctgat ttactgttct cttctgtcgt caccgtactc tcctcatcat cctgggtatc
    46441 ccagaatttc ttgatcatcg cggttagcgc cggaaccgaa agcggcttac tcagcacatc
    46501 atccattcca gcattgaggt actcttgttt gtctttcagc acgttagcgg ttaaggccac
    46561 cagcggcggt aaatcctcgc gcggataacg cttcgtcagt tcacgagaga tatccagccc
    46621 ggtcatatct ggcaactgaa tatccagcaa caccaggtcg tattcgcccg gtttaaacat
    46681 ctccagcgcc gccttgccgg tcatggcgac atcaacgctg ttacctaatt tttccagcac
    46741 agaacgcgca acaatcacgt tcagttcaat gtcttccacc agcagcacat tcagcgccgg
    46801 taaaggcata tcgtcttcat caaacgcatc atcgacctct tctgctaccg acggtgcgtg
    46861 gatcgtcaac gtaaaggttg aacctttgcc ctgttcgctg gtaaccgtaa tatcgccgcc
    46921 catatttttc gccagacgac gagaaacggc cagaccaata ccggtgccgg tggcaggttt
    46981 accgccatga ctgtctttca cctggtaata catggcgaaa attttatcca gctcatcctg
    47041 cggaatgccg ataccagagt cttccacttc aaaatgcagc atatcgcctt catcgtagcg
    47101 cacgcgcacg gtaacctggc cttgctgggt gaatttgacg gcgttactga tgaggttcca
    47161 caggatctgc cgtaaacgcg tcccgtcggt aatgacctga tgcggtaatg gcagcgtcgg
    47221 ctccaggtta aagcgcaatc ctttttgttg cgcctgcaat gcggagagat tttccagatc
    47281 ggcaaggaag ctggtgaaat caaccggttg attatcaagc tggaccttgc gccgttccat
    47341 cttatccatg tcgataatat cgttaaagat attccccagc gtgacggccg aaacatggat
    47401 ggtcttgaga tatttttcct gctcggcggt gagttcggta tccagcagaa tacggctcag
    47461 gccgacgata ccgttcagcg gtgtacgcaa ttcgtgactg atggtggaga taaacgtcgt
    47521 tttgtcgcgg ctggcccgtt caagcgcatc ctgataccgc ttacgctcgg taatgtcgcg
    47581 accaaagccc atcaaaccgt gacgtttacc cacgcggtcg tagtacggca ctttacggat
    47641 ttcaaagcag gctttgcgcc cgtccgggta atccagccac tgttcatagg tcagtgacac
    47701 attatgacgg aacacttttt catcggtttc aatgactttt gcggcggctt ccggtgagta
    47761 aacatcagca ggtttcaggt gaaccagttg tttttcgctt tttccggtca gcagctccat
    47821 cgcgcggtta cagccggaaa actctttatc ttcgttacga taaaaaacca ggtcgggtga
    47881 agcatcaagg aaggaacgta agaatgagga ttgctgctcg agctgaattt gtgtctcttc
    47941 gcgctctttg atttcaattt tcagttggcc gaaggtttcc tgcagttctg cttccgcttt
    48001 ttcacgaacg gcgatttcct gatttagctg ggcaatatta tcttttaact gaacgttgag
    48061 gctcaaatcg cgctcgcgca tctcctccag tttttgcacc agccgtgaca gacgttgtcg
    48121 tgactcctcc agttgctcga cgaccaccga tagaaagtag accgcccacg gcgtaatcag
    48181 caaaccaaag aagatagaac gaataacatc aatgctttcg acctgaccat gcagcaccat
    48241 ggttaccgcc atttgtacca caatggcaag aacgacgagg gccagcgcca gcaacattga
    48301 gaagcgcacc agacctaact tcatcatcag gtcaacataa tactgcgcca gcagacgaat
    48361 ttgcttcatt agggaattcc ttcacgacaa cctgccccaa taatacccaa ttctgacagt
    48421 tacgttataa attgtgtgag aaatgcggaa ttcccctcaa cgacctactc cgttggaggt
    48481 atccacggac gttccagcgc cgatccctgt acgccatgtt cattcagata gcgatccagc
    48541 tcgaccattc ccgtccatcg attttcgcac cacagaggag caagcagcgt cgggcgacgg
    48601 gcgctggcgg aaatgcggtg gtagatcact tccggtggcg tatggcgaat catttctccg
    48661 gcagtgagcg tgtaatcctc cagttcaata ccgttcaaac gccccgcttc ccaggctttc
    48721 gccataatgc tgcctttcac aatatgcagc ggatgcagct ttatgccgtc cacgccggtt
    48781 tcaaccactc gctccagcgt ttgcaaacat tcagcctgcc cttcgccagg caggccgaca
    48841 attaagtggg aacacacctt cagcccgcgc tgacgtgcca gctgggttgt acgctgataa
    48901 caggcaaaat catggccgcg gttgatgcga tgcagtgttt tgtcgtgggc ggtttgtagc
    48961 cccagctcca gccacacttc gtaaccctgg tccttatatt cgcaaagcag atccagcacc
    49021 gcatccggca cgcagtccgg gcgggtacca acacacaaac cgacaatatt ggcctggctc
    49081 accgcctgct gatacataga acgcagcacc tgaacttccg caaaggtgct ggtatacgcc
    49141 tgaaagtagg ccagatagcg tttagcgcgg ttaactaaat tcgcctggtg cgccagttgc
    49201 tcggcaatgg aacgatgctg ctgcgcttca tcggcaaacg aggcaacatt acagaatgtg
    49261 cagccaccac gcccgatggt accgtcacgg ttagggcagc taaaaccgcc gtgcagcgtc
    49321 agcttatgca ccttttgccc ataacgacgg gtgagatcac caccaaacat attgactaat
    49381 ttctgtaact gcataatctg atagaccgcg ccttgaaaag aggccaaagc ctgccatttt
    49441 tagctcaatt cggcgatgac ctggatcaat cgtcccggcc tgcttttatc aactgcataa
    49501 tcaatcaaaa ttaccgaaat ttcatgcata atcacataaa tcacttttgc ttatcttgtg
    49561 tcagattttt ttatctcctt atggatttta ggcaaaaaca gtagcatgaa acgtcattac
    49621 caattaaggc agtataaaat gctggctttc tcgtcagttc aaggcaggat aagggttaac
    49681 acgcctttat gacagtcagg aattgactgt ttctctaacg acttcccttt tagccttaaa
    49741 gataaaatct attttaattt cagtcattta ataaagaatt ttgcgctaaa gcacatttct
    49801 gtaccaataa gcttgccatt tgacctgtat cagctttccc gataagttgg aaatccgctg
    49861 gaagctttct ggatgagcag cctgctcatc atatttatgc agtaattgag atcccctctt
    49921 caccgtatta accgatgcga aaaggacaac aagggggcga atgcgaggcg cgcgtatgac
    49981 acgcaaaccc cgtcgccacg ctctttctgt gcccgtgcgc agcggttcgg aagtggggtt
    50041 cccgcagagc ctgggggagg ttcacgatat gttgtacgat aaatcccttg agagggataa
    50101 ctgtggtttc ggcctgatcg cccacataga aggcgaacct agccacaagg tagtgcgtac
    50161 tgcaatacac gcactggccc gcatgcagca ccgtggcgcg attctcgccg atggtaaaac
    50221 cggcgacggt tgcggcttgc tgttacaaaa accggatcgc ttttttcgca tcgttgcgca
    50281 ggagcgcggc tggcgtttag caaaaaacta cgccgtcggg atgctattcc tgaataaaga
    50341 tcctgaactc gccgctgccg cacgccgcat cgttgaagaa gaactgcaac gcgaaacctt
    50401 gtcgattgtg ggctggcgtg atgtccccac taacgaaggc gtgctgggtg aaatcgccct
    50461 ctcctctctg ccacgcattg agcaaatttt tgtgaacgcc ccggcaggct ggcgtccgcg
    50521 cgatatggag cgccgtctgt ttatcgcccg ccgccgcatt gaaaagcgtc tcgaagccga
    50581 caaagacttc tacgtctgta gcctgtcgaa tctggtgaac atctataaag gtctgtgtat
    50641 gccggcggat ctgccgcgct tttatctgga tcttgcggac ctgcgtctgg aatcggccat
    50701 ctgcctgttc caccagcgct tctccactaa caccgtaccg cgctggccgc tggcgcagcc
    50761 gttccgctac ctggcgcata acggtgaaat caacaccatc accggtaacc gccaatgggc
    50821 gcgtgcgcgt acctataaat tccagacgcc gcttatccct gatctgcacg acgccgcacc
    50881 gttcgtcaac gaaaccggct ctgactccag ttcgatggat aacatgctgg aactgctgct
    50941 ggcaggcggg atggatatca tccgcgccat gcgtctatta gtaccacccg cctggcagaa
    51001 caacccggat atggacccgg aactgcgtgc cttctttgac tttaactcca tgcatatgga
    51061 gccgtgggat ggcccggcgg gcatcgtgat gtccgatggt cgcttcgctg cctgtaacct
    51121 cgaccgtaac ggtctgcgtc cggcacgcta cgtcattacc aaagacaagc tcatcacctg
    51181 cgcctctgaa gtcggtatct gggattatca gcctgacgaa gtggtcgaaa aaggccgcgt
    51241 cgggccaggc gaactgatgg tgatcgacac ccgcagtggg cgtattctgc actcggcaga
    51301 aaccgatgac gatctgaaaa gccgccatcc atataaagag tggatggaga aaaacgtccg
    51361 ccgactggta ccgtttgaag atctgcccga tgaagaagtg ggtagccgcg aactggacga
    51421 cgacacgctt gccagctacc agaaacagtt taactacagc gcggaagagc tggactccgt
    51481 aattcgcgta ctgggcgaaa acggtcagga agcggtcggt tcgatgggcg atgatacccc
    51541 attcgccgtg ctctccagtc agccgcgcat tatttacgac tacttccgcc agcagtttgc
    51601 ccaggtgact aacccgccaa tcgacccgct gcgtgaagcg catgttatgt cgctcgccac
    51661 cagtatcggt cgtgaaatga acgtcttttg cgaagcagag ggccaggcgc accgtttaag
    51721 ctttaaatcg ccgattctgc tctactccga tttcaaacag ctcacgacga tgaaagagga
    51781 gcactaccgc gcagatacgc tggatatcac ctttgacgtc actaaaacca cgctcgaagc
    51841 gacagtcaaa gagctgtgcg acaaagccga aaaaatggta cgtagcggca ccgtgctgct
    51901 ggtgctctcc gaccggaata tcgctaaaga tcgcctgccg gttccagccc cgatggcggt
    51961 tggcgcgatc cagacccgtc tggtcgatca aagcctgcgt tgcgatgcca acatcatcgt
    52021 cgaaaccgcc agcgcccgcg atccgcacca cttcgccgtg ttgctgggct tcggcgcgac
    52081 ggctatttat ccataccttg cctatgaaac gctgggccgc ctggtagaca cccatgcgat
    52141 tgccaaagat tatcgtaccg tgatgctcaa ctaccgtaac ggcatcaaca aaggcttgta
    52201 caaaatcatg tccaaaatgg gcatctccac catcgcctct taccgctgct cgaaactgtt
    52261 tgaagcggtc ggtctgcacg atgatgtagt gggcctgtgc ttccaggggg cggtcagccg
    52321 cattggtggg gcgagctttg aagacttcca gcaggatctg ctgaacctgt cgaaacgtgc
    52381 ctggctggcg cgtaagccca tcagccaggg cggcctgctg aaatacgtcc acggcggcga
    52441 ataccacgct tacaacccgg acgtggtgcg cacgctgcaa caggcggtac aaagcggcga
    52501 gtacagcgac tatcaggaat acgcgaagct ggttaatgag cgtccggcaa ccacgctgcg
    52561 cgatctgctg gcaattacgc cgggtgaaaa cgcggtcaac attgctgatg ttgaaccggc
    52621 aagcgaactg tttaaacgct ttgataccgc cgcgatgtct atcggcgcgt taagcccgga
    52681 agcccacgag gcgctggcgg aagcgatgaa tagcatcggc ggtaactcga actccggtga
    52741 aggcggcgaa gacccggcgc gctatggcac taacaaagtg tcgcgcatca agcaggtggc
    52801 ttctggtcgc ttcggcgtta ctccggcgta tctggtcaat gccgacgtca ttcagattaa
    52861 agtcgcccag ggcgcgaagc cgggcgaagg cggtcagttg ccgggcgaca aagtcactcc
    52921 ttacatcgcc aaactgcgct attcggtgcc cggagtgacg ctgatctccc cgccgccgca
    52981 ccacgatatc tactctatcg aggacttagc gcagctcatt ttcgacctca agcaggttaa
    53041 cccgaaagcg atgatctccg tgaagctggt ttccgaaccg ggcgtaggca ccatcgcgac
    53101 tggcgtggca aaagcttatg ccgatttaat caccatcgca ggctatgacg gtggcaccgg
    53161 cgcaagcccg ctgtcgtcag tgaaatacgc aggttgcccg tgggagctgg ggcttgttga
    53221 aacccagcag gcgctggttg ccaatggtct gcgtcataag atccgtttgc aggtcgatgg
    53281 cggcctgaaa accggtgttg atatcatcaa agcggcgatt ctcggcgcag aaagcttcgg
    53341 cttcggcact ggcccgatgg tggcgctcgg ctgtaaatat ctacgtattt gccatctgaa
    53401 caactgcgca acgggtgtag caactcagga tgacaaactg cgtaagaacc actatcacgg
    53461 cctgccgttc aaggttacca attactttga atttattgcc cgtgaaaccc gcgagctgat
    53521 ggcacagctg ggcgtaacgc gtctggtgga tctgattggt cgcaccgacc tgctgaaaga
    53581 actggacggt ttcaccgcca aacagcaaaa actggcgctg tcgaagctgc tggagaccgc
    53641 cgaaccgcat ccaggtaagg cactctactg caccgaaaac aacccaccgt ttgataacgg
    53701 cctgctgaac gcgcagttgc tgcaacaggc gaaaccgttt gtcgatgaac gccagagcaa
    53761 aaccttctgg ttcgatattc gcaacaccga ccgttctgtc ggcgcgtcgc tttcaggcta
    53821 tatcgcccag acgcacggcg atcaggggct ggcagccgat cctatcaaag cgtacttcaa
    53881 cggcaccgca ggccagagtt tcggtgtgtg gaacgcgggc ggcgtggaac tgtacctgac
    53941 cggggatgcc aacgactatg tcggtaaagg catggcgggc ggcttaatcg ccattcgtcc
    54001 tccggttggt tccgccttcc gcagccatga agcaagtatt atcggcaaca cctgcctgta
    54061 tggcgcgacc ggtggtcgtc tgtatgccgc aggccgcgcg ggtgaacgtt tcggcgtgcg
    54121 gaactccggt gctatcaccg tggtagaagg cattggcgac aacggctgtg aatatatgac
    54181 gggcggtatt gtctgtattc tgggtaaaac cggcgttaac ttcggtgcgg gcatgaccgg
    54241 cggcttcgct tacgttctcg atgaaagcgg tgatttccgc aaacgcgtta acccggaact
    54301 ggtcgaggtc ttaagcgttg acgatctggc gatccatgaa gagcatctgc gcggtcttat
    54361 caccgagcat gttcagcata ctggctctca gcgcggtgaa gagattctgg cgaactggtc
    54421 aaccttcgcc actaaatttg cgctggttaa accgaagtcc agtgatgtaa aagcactgct
    54481 gggtcatcgt agtcgtagcg cagcagagtt gcgcgtgcag gcgcagtaag gggtagcaac
    54541 aatgagtcag aatgtttatc aatttatcga cctgcagcgc gttgatccgc caaagaaacc
    54601 gctgaagatc cgcaaaattg agtttgttga aatttacgag ccgttttccg aaggccaggc
    54661 caaagcgcag gctgaccgct gcctgtcgtg cggcaaccca tactgcgagt ggaaatgtcc
    54721 ggtacacaac tacatcccga actggctgaa gctcgccaac gaggggcgta tttttgaagc
    54781 ggcggaactg tcgcaccaga ccaaccccct gccggaagtt tgcggacgag tctgcccgca
    54841 agaccgtctg tgcgaaggtt cctgcactct gaacgatgag tttggcgcgg tgaccatcgg
    54901 caacattgag cgttatatca atgataaagc gttcgaaatg ggctggcgtc cggatatgtc
    54961 cggcgtgaag caaaccggta aaaaagtggc gattatcggc gcaggcccgg caggtctggc
    55021 gtgtgcggat gtcctgacgc gtaacggcgt aaaagccgtt gtcttcgacc gtcatccaga
    55081 aattggcggg ctgctgacct tcggtattcc ggccttcaag ctggaaaaag aggtaatgac
    55141 gcgtcgccgt gaaatcttca ccggcatggg tattgaattc aaactcaata ccgaagtggg
    55201 ccgcgacgtg cagctggacg atctgctgag tgattacgat gccgtcttcc ttggcgtcgg
    55261 gacttatcag tcaatgcgcg gcgggctgga aaacgaagac gccgatggcg tgtacgcagc
    55321 gctgccgttc cttatcgcca acaccaaaca gttaatgggc tttggtgaaa cccgcgacga
    55381 accgttcgtc agcatggaag gcaaacgcgt ggtggtcctt ggcggtggcg acactgcgat
    55441 ggactgcgtg cgtacgtccg tgcgccaggg agcgaagcac gttacctgtg cctatcgtcg
    55501 tgatgaagag aacatgccgg gttcccgccg cgaagtgaaa aacgcgcggg aagaaggcgt
    55561 agagttcaaa ttcaacgtcc agccactggg gattgaagtg aacggtaacg gtaaagtcag
    55621 cggcgtaaaa atggtacgta ccgaaatggg cgaaccggat gccaaaggcc gtcgccgcgc
    55681 ggagatcgtt gcaggttccg aacatatcgt tccggcagat gcagtgatca tggcgtttgg
    55741 tttccgtcca cacaacatgg aatggctggc aaaacacagc gtcgagctgg attcacaagg
    55801 ccgcatcatc gccccggaag gcagcgacaa cgccttccag accagcaacc cgaaaatctt
    55861 tgctggcggc gatatcgtcc gtggttccga tctggtggta accgctattg ccgaaggtcg
    55921 taaggcggca gacggtatta tgaactggct ggaagtttaa gctctgtccc agcgccgcct
    55981 cctcagaaaa gagtcggcgc tgaaattctc ctttctttct ggaaagcgcc tcgcataatc
    56041 tgaagaaaac caatgtaata cgctcatctg gattgttacc ggtagatgcc cggcgtatag
    56101 ctgattccat tattcaacgc agcgccatat aaagggacag ataatggaat cgctctcaga
    56161 gagaacctca acaggctacc agcaaatcca cgacggtatt attcatctgg tcgatagcgc
    56221 ccggacggaa acggtacgta gcgttaacgc gctgatgacc gcgacatact gggaaattgg
    56281 ccgacgaatt gtcgaatttg aacaaggtgg cgaggccagg gctgcgtatg gtgcgcagct
    56341 aatcaagcga ctatcaaagg atttaagtct aaggtataag cgtgggttct ctgcaaaaaa
    56401 cttacgccaa atgaggcttt tttacctctt ttttcaacat gttgaaattc gccagacagt
    56461 gtctggcgaa ttaacacctg ccctggtcca cctacgtccg tttactttcc gttaaaaacg
    56521 ctgacgcccg cagcttttat gaaaaagaga cgctccgctg tggctggtct gttcgccagc
    56581 tagaacggca aattgcgacc caattttatg agcgaacact actgtcacat gacaaatcag
    56641 ccatgctgca acaacacgct cctaccgaga cgcatattct tccgcaacag gcgatacgcg
    56701 atccctttgt gctcgaattt ctggaattga aagatgaata ttcagaatct gatttcgagg
    56761 aggcgctgat caaccacctg atggatttca tgctggaact tggggatgat tttgcctttg
    56821 ttggtcggca gcgaaggtta cgcattgatg acaactggtt tcgtgtcgat ctgctgtttt
    56881 tccaccgccg tttacgctgc ctactaatcg tcgatctaaa agtgggcaaa ttcagctata
    56941 gcgatgccgg acagatgaat atgtatctca actacgccaa agagcactgg acgctaccgg
    57001 atgaaaatcc gcccatcggt ctggttctct gtgcagagaa aggagccgga gaagcacatt
    57061 atgctctggc aggtttgcct aacaccgttc tggcaagcga atataagatg caactacctg
    57121 atgagaaacg actcgcagat gaactcgttc gaacacaggc ggtgctagag gaaggctata
    57181 gacgccgtta atttcagcgg tttcgaattc tgcagacagt gtctgcagaa tctttaaaac
    57241 gaactaagtt ttaagccatt aaatcagcct taaccttcac cacaaccttt ttaatctctc
    57301 caggctcgcc gacaacgcat cctggtttat gcggttcacc tggcataaac acggcgaaca
    57361 ttcccggttt taagatgatg gcttgctcgt tatcaatggt gctgcaaagc tgataatcat
    57421 cctcatggtg gaactcttca cactggcgcg cagtgcctgc catgccaaac agaatccgtt
    57481 cctcaccgtt taataacagt tggatatcaa tgtattgctc gtgcaattcc gcttttttct
    57541 cgacgggcga ttgagtgtta aacgtcatga cattcataaa gatattgtcg ccctgtaatt
    57601 cgtaacgacc cggcgctttt tcttgcggtc tggcagctaa tgccagcgtt aacgcgtcct
    57661 gtaacgcagg atgtaaccca gcagacggta atgactgtac ttcacccatc atcataattt
    57721 ttctccctgg gccaacagcg cagccccaag taaacctgca tcgtggcggt agtgcgccgc
    57781 cagtaaatca acatgaaatg ccgctggctc ctgcgccaga tacgtttcca ccagcgccag
    57841 atacccttct gccagcccaa cgctgccacc gaccaccacg cactggcaat cagttgtggc
    57901 tttaatatca gcgatcagcc ttgcaagcgt acgtgcggag cggtgaatca gctgctgcgc
    57961 ctgctcgtcg ccctgcccgg cgcgtgtgaa aatagttctc gcatccgcgc ccgccagttc
    58021 accctgcgct gccgccgcaa tgccgcgacc agaagcaatc gcctccacgc aacccgtgcg
    58081 tccacagcca cagaccgggc cgtgtggatc ggcaagcgta tgcccgatgt gccccgccag
    58141 accgccaggg ccggtaagca gtttgccgcc gctcactaca ccgccgccga cgccagtgga
    58201 aacggtgata aagaccatat cggttatatc gccttccagc gcctgatact ctgcccatgc
    58261 tgcggcctgc gcgtcgttaa tggcaatggt cggcaaattg gtaagttgtt ccagcgtttt
    58321 gactaacgga aagtgtagca atccaccgag attatgcgga ttcagcgcca gcaaactgcc
    58381 gtcgcgaata attccggttg aagcgatggc aacccgctgc gcatgagctt gcaacggaga
    58441 gactaatgcg gataaggcat cacgcaaggc ttctggtgtc tggctggctg gcgtaggaag
    58501 ttcacgacga tcgcggatct gcccgtcagc gccaatcagc gcggcggcaa gtttagtacc
    58561 gccgatatca atcgccagcg tggtcatagc accgcctttt tcatcgctgt gttgtaccac
    58621 tgacaaatgt gttcaagacg cgtaatggca gaaccgaccg tcaccgccca cgcgccgtgg
    58681 cgcatcgcat ctgccgcctg agcaggcgtg ttgtaacgcc cttcggcaat caccctacat
    58741 ccggcgtcgc tcaacgtttt caccagcgcc agatccggct cttctggcgt ttcaggcgtg
    58801 gtatagccag aaagcgtagt gccaataatt tcggctccca gcttttggca tgccaggccg
    58861 tcttccggcg ttgagcagtc ggtcatcgcc agtaaaccgt gatggtgaat acgtgccagc
    58921 agcgtttcaa caggcaccgg acgcgggcgg tcggtgccgt caatggcgat aatgtccgcg
    58981 cccgcctgcg ccagcgcatc aacatcttca atataggccg tgatgcgtac cggagaatcc
    59041 tccagatcgc gtttcacaat tccaataatc ggcacgctca ccaccgcacg cgtggcttgc
    59101 agatttgcca caccttcaat gcgaatggca accgcgcccg cctgttctgc cgctaatgcc
    59161 atggcggcga cgatttcggg tttatcgagc gggctgtccg gaaccggctg gcaggagaca
    59221 atcaggccac cgttagcagc gattttttga tccagttgtg caagtaacga catacatctt
    59281 cccttagcga aaggcccggt acatagaccg ggcaacagga ttaacttttg gttttgacta
    59341 aatcgttttt ggcgctgcca aatggcacgg caccgctaaa tggtttgccg tcgatagcgt
    59401 catgagtacg caatgcttcc gggcgcagcc aacgctgaac gcgggaaggc atatccagac
    59461 caatcagcag gatcaccaca aatgtcagac tgaacgatag cgatgccagc gcagtaccca
    59521 gatccagacg ttgagcgatc aacgcgccga tgattggggc cagtgcgccg cccaatgcac
    59581 caacgttgta ggtaaagccc aggcccgctg cacgctggtc ggtatcgaaa taaccgccaa
    59641 tcagtttcgg taagatcccg gcgatccctt gtccaagcat ttgctggaag aacagtaaca
    59701 gaccgagcac ccagacgttt gcgccgccaa tcgcaaatac cggaataatc aacagctgcg
    59761 aggccaacag gctacaaacg tacgctttgc gggttcccag ccagtcaccg aggaagccgc
    59821 cgacacagca ccccaccgca gccccaaagc cgctaaagaa cagcacattg gcgacagtat
    59881 gcgggttata agccagatcg gttttcagat acgttggcag cagcgcctga atcggccatg
    59941 agtagaggaa agcaaacaac acgaccacca tcagcattac gcccgttggc cagcgtttac
    60001 ccgtactttg caccataaag ctgataaaga ttgcggcgca taacagccca agaacagcga
    60061 cgatcgcggc attttgcagg ttaccggcga agcaaaacca cagcgcagta gccgccgcca
    60121 gtgtcattac gatattggca atgcgatgtt caccacggta gagaatatct accatcgtgc
    60181 gtactggtgc tttacctgca tgtttctctt tccagtcttc cgcttccggg atgtttttac
    60241 gcagccagag agcaaagatg ataggcaaaa tgccgataaa gaacagcgca cgccagcccc
    60301 agaccggaac caccaggcta tagacctgag cggcaacgac ggcccccaca gagaagcctg
    60361 aaatcagaaa accactggct ttgttacgca gatgttttgg ccagctttca atgacatagg
    60421 tggcgctgga accgtattca cccgccatcc ccatgccgat gaccagacgc gcgataaaca
    60481 tggtgatgta gcctggcgca aagccgcagg ccagcgtccc ggccgagaag agaacgatgc
    60541 tggtgaccat tgccagacga cgcccgtagc ggtcacccat agcgccgagc atcaggccgc
    60601 cgaaccagcg agagataaag gctgcggaga tcagacttgc cgcctgcacc gtcgtcagcc
    60661 cgaattcacc ttgtacttcg gtgagtacca gggcgattaa aacgaaatca aaaccgtcaa
    60721 gcagatatcc caaccaggca gcggaaaatg cccgccattg tgcacgattg agatggcgat
    60781 accacgggat attctgggtt gtagtactca ttgtgagtct cccgcggtgg gcgatgccca
    60841 cacgctttgg tatgaaaatt gtagggtaca gatgcgttta tttcccctca cccggtaggg
    60901 gcgagcgagg ggaaaacaac tcacccgcgc tcttgcatca actgctgggc cagcgccttc
    60961 agttctggca gatatttttc atctaccggt ccaaacggtt tgcggcacag cggcacagaa
    61021 acgacatcca tataatggag gacagttttc aggccgcgga atacgcccgt tttgatcagt
    61081 aaatcaatga ctttattgca ttcagtttgc agtttctgcg cggtctggat atcgccttct
    61141 ttcagcgcct taacgatccc ctgatagcgc cagcccatga tgttgtaggt actaccgata
    61201 ccaccatcag cgcccgccag cagaccagag gcgaagattt cgtcgtaacc gttatagagc
    61261 acaagatcag gatgttcacg acggatctgc tccatctgat agagatcgcc agaagtctgt
    61321 ttcagcgcac cgacgccagg caacgtaacc agggtgttga tctgatccag ggtcagtttt
    61381 accccactca gggctggaat gttgtacacc accatcggca aaccatccgc tgaatcaata
    61441 attgcccgat agtgatcgca gtgttcttca aagctgaaag gatagtagaa cggcgtgacg
    61501 gcggagacgg catcgaagcc ataacgttta gccgatgccg caagttgttg gctttcggcg
    61561 gtgctgacgc aaccgacgtg ggcgatgagt ttaatcttac ctttcgcctc ttcggcgacg
    61621 atttccagta cctgttcacg ctcggaaagg ctttgtacaa aggcctcgcc ggtcgaacca
    61681 cccacgtata aaccgtcgat gccctgctga atattgaact gaaccaggcg acgcagactc
    61741 gctttatcca gtgcttgttg ttgatcaaaa ggagtcagga gtgcagccat tacgccacgt
    61801 aaattcgttg ccataaatac ctctgaagtg atgcttgtct gataaacgat atacctttat
    61861 acctgttata ccagatcaat taagcaacac cccatacaga aagcttataa tgcgatctgc
    61921 ttcactaaag tggcattatt tctttttgtt ggtggtctga ccgaaagcgt gccaggtagc
    61981 agagacgctg ttgagatgcg attgcaacgc acgatcggct tcgtcaggat catgacggcg
    62041 gatcgcatca acgatcgcaa tatgctgttg ataactaacg ttgttatgtt cgtgcagtgc
    62101 ctgatcggta accgttgggc gtgcggcaat aagccagtcg agcagggcaa cgtggatcgc
    62161 catgaagatt gggttaccgg ggatctccgc cagcacgcgg tggaaatcaa cgtctgaacg
    62221 aatgaatgcc gcgttgttat ccagcgactg actgttaatt tccagtgctt ttgccagcaa
    62281 atcgatttgc tcatcggtgg catgttcagc cgcatagcgc accaggctgg attcaaagaa
    62341 cagacgtaat tgttcgaaat gggcaatccc accaggatga gaaaggaaat ctttcgccat
    62401 gccggaaagc tcgccgatga tggtgtccgc agaaggacgt gagacgcggg cacgttcgcc
    62461 gttgttgatt tgcaccagac ctttgcgttt taacgctgcc agcgcttcgc gcacagaagg
    62521 acgcccgacg ttaaagaacg ccatcagttc gcgttcagac ggtaattgtt caccttcgcc
    62581 aaattcacga cggcggatca tctgttccag ctcttcttcc accatttcgg agagtttttt
    62641 acgcgccagc gggcggctac gcaagttgcg accaattgca ggtgaagaat cttcggtttg
    62701 cgaatcaaat gcgttcataa ggcccattct gtaaggtcag tgtgattaac atcatcagtg
    62761 acatcctatc acaggattga aagtagggga aaatggcagg tttttctctt tgtgcctcat
    62821 cattaccata attaacggaa taattaacta ttgcgaaaaa tt
//
LOCUS       DAZGJW010000010        58623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZGJW010000010 DAZGJW010000000
VERSION     DAZGJW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.010"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..165
                     /gene="yhfL"
                     /locus_tag="ACHKIK_000682"
     CDS             <1..165
                     /gene="yhfL"
                     /locus_tag="ACHKIK_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO9081910.1"
                     /translation="NKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISK
                     IIGGCGPTAQ"
     gene            460..1797
                     /gene="frlA"
                     /locus_tag="ACHKIK_000683"
     CDS             460..1797
                     /gene="frlA"
                     /locus_tag="ACHKIK_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO9081911.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            1818..2840
                     /gene="frlB"
                     /locus_tag="ACHKIK_000684"
     CDS             1818..2840
                     /gene="frlB"
                     /locus_tag="ACHKIK_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO9081912.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGQLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2890..3720
                     /gene="frlC"
                     /locus_tag="ACHKIK_000685"
     CDS             2890..3720
                     /gene="frlC"
                     /locus_tag="ACHKIK_000685"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO9081913.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            3717..4502
                     /gene="frlD"
                     /locus_tag="ACHKIK_000686"
     CDS             3717..4502
                     /gene="frlD"
                     /locus_tag="ACHKIK_000686"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFO9081914.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSSLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            4602..5333
                     /gene="frlR"
                     /locus_tag="ACHKIK_000687"
                     /gene_synonym="yhfR"
     CDS             4602..5333
                     /gene="frlR"
                     /locus_tag="ACHKIK_000687"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO9081915.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5485..6570)
                     /gene="yhfS"
                     /locus_tag="ACHKIK_000688"
     CDS             complement(5485..6570)
                     /gene="yhfS"
                     /locus_tag="ACHKIK_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO9081916.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(6582..7886)
                     /gene="yhfT"
                     /locus_tag="ACHKIK_000689"
     CDS             complement(6582..7886)
                     /gene="yhfT"
                     /locus_tag="ACHKIK_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO9081917.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(7898..8251)
                     /gene="yhfU"
                     /locus_tag="ACHKIK_000690"
     CDS             complement(7898..8251)
                     /gene="yhfU"
                     /locus_tag="ACHKIK_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO9081918.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(8262..9140)
                     /gene="php"
                     /locus_tag="ACHKIK_000691"
     CDS             complement(8262..9140)
                     /gene="php"
                     /locus_tag="ACHKIK_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO9081919.1"
                     /translation="MSIDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(9137..10363)
                     /gene="yhfW"
                     /locus_tag="ACHKIK_000692"
     CDS             complement(9137..10363)
                     /gene="yhfW"
                     /locus_tag="ACHKIK_000692"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO9081920.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPALEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIGRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVHTQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(10363..11526)
                     /gene="yhfX"
                     /locus_tag="ACHKIK_000693"
     CDS             complement(10363..11526)
                     /gene="yhfX"
                     /locus_tag="ACHKIK_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO9081921.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPMLAEYGVTHAEPGHALTGTIPANQQGDQPEYIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(11610..11972)
                     /gene="yhfY"
                     /locus_tag="ACHKIK_000694"
     CDS             complement(11610..11972)
                     /gene="yhfY"
                     /locus_tag="ACHKIK_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO9081922.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(11989..12894)
                     /gene="yhfZ"
                     /locus_tag="ACHKIK_000695"
     CDS             complement(11989..12894)
                     /gene="yhfZ"
                     /locus_tag="ACHKIK_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO9081923.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGFCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(13163..14167)
                     /gene="trpS"
                     /locus_tag="ACHKIK_000696"
     CDS             complement(13163..14167)
                     /gene="trpS"
                     /locus_tag="ACHKIK_000696"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO9081924.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(14160..14918)
                     /gene="gph"
                     /locus_tag="ACHKIK_000697"
     CDS             complement(14160..14918)
                     /gene="gph"
                     /locus_tag="ACHKIK_000697"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO9081925.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(14911..15588)
                     /gene="rpe"
                     /locus_tag="ACHKIK_000698"
     CDS             complement(14911..15588)
                     /gene="rpe"
                     /locus_tag="ACHKIK_000698"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO9081926.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKLSHE"
     gene            complement(15606..16442)
                     /gene="dam"
                     /locus_tag="ACHKIK_000699"
     CDS             complement(15606..16442)
                     /gene="dam"
                     /locus_tag="ACHKIK_000699"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO9081927.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(16549..17835)
                     /gene="damX"
                     /locus_tag="ACHKIK_000700"
     CDS             complement(16549..17835)
                     /gene="damX"
                     /locus_tag="ACHKIK_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO9081928.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(17927..19015)
                     /gene="aroB"
                     /locus_tag="ACHKIK_000701"
     CDS             complement(17927..19015)
                     /gene="aroB"
                     /locus_tag="ACHKIK_000701"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO9081929.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADSQSA"
     gene            complement(19072..19593)
                     /gene="aroK"
                     /locus_tag="ACHKIK_000702"
     CDS             complement(19072..19593)
                     /gene="aroK"
                     /locus_tag="ACHKIK_000702"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO9081930.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(19994..21232)
                     /gene="hofQ"
                     /locus_tag="ACHKIK_000703"
     CDS             complement(19994..21232)
                     /gene="hofQ"
                     /locus_tag="ACHKIK_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO9081931.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(21144..21548)
                     /gene="hofP"
                     /locus_tag="ACHKIK_000704"
     CDS             complement(21144..21548)
                     /gene="hofP"
                     /locus_tag="ACHKIK_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO9081932.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(21538..21978)
                     /gene="hofO"
                     /locus_tag="ACHKIK_000705"
     CDS             complement(21538..21978)
                     /gene="hofO"
                     /locus_tag="ACHKIK_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO9081933.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(21962..22501)
                     /gene="hofN"
                     /locus_tag="ACHKIK_000706"
     CDS             complement(21962..22501)
                     /gene="hofN"
                     /locus_tag="ACHKIK_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO9081934.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(22501..23280)
                     /gene="hofM"
                     /locus_tag="ACHKIK_000707"
     CDS             complement(22501..23280)
                     /gene="hofM"
                     /locus_tag="ACHKIK_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO9081935.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            23400..25952
                     /gene="mrcA"
                     /locus_tag="ACHKIK_000708"
     CDS             23400..25952
                     /gene="mrcA"
                     /locus_tag="ACHKIK_000708"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO9081936.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(26118..26678)
                     /gene="nudE"
                     /locus_tag="ACHKIK_000709"
     CDS             complement(26118..26678)
                     /gene="nudE"
                     /locus_tag="ACHKIK_000709"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO9081937.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            26998..29133
                     /gene="igaA"
                     /locus_tag="ACHKIK_000710"
     CDS             26998..29133
                     /gene="igaA"
                     /locus_tag="ACHKIK_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO9081938.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWVPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            29198..29866
                     /gene="yrfG"
                     /locus_tag="ACHKIK_000711"
     CDS             29198..29866
                     /gene="yrfG"
                     /locus_tag="ACHKIK_000711"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO9081939.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            29877..30278
                     /gene="hslR"
                     /locus_tag="ACHKIK_000712"
     CDS             29877..30278
                     /gene="hslR"
                     /locus_tag="ACHKIK_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO9081940.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            30303..31181
                     /gene="hslO"
                     /locus_tag="ACHKIK_000713"
     CDS             30303..31181
                     /gene="hslO"
                     /locus_tag="ACHKIK_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO9081941.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILTEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(31316..33040)
                     /gene="yhgE"
                     /locus_tag="ACHKIK_000714"
     CDS             complement(31316..33040)
                     /gene="yhgE"
                     /locus_tag="ACHKIK_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO9081942.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYKSVWHNVLILLVIFLANGLTWVVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            33419..35041
                     /gene="pckA"
                     /locus_tag="ACHKIK_000715"
     CDS             33419..35041
                     /gene="pckA"
                     /locus_tag="ACHKIK_000715"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO9081943.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKELGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(35117..36469)
                     /gene="envZ"
                     /locus_tag="ACHKIK_000716"
     CDS             complement(35117..36469)
                     /gene="envZ"
                     /locus_tag="ACHKIK_000716"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO9081944.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIDVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(36466..37185)
                     /gene="ompR"
                     /locus_tag="ACHKIK_000717"
     CDS             complement(36466..37185)
                     /gene="ompR"
                     /locus_tag="ACHKIK_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO9081945.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            37413..37889
                     /gene="greB"
                     /locus_tag="ACHKIK_000718"
     CDS             37413..37889
                     /gene="greB"
                     /locus_tag="ACHKIK_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO9081946.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            37986..40307
                     /gene="yhgF"
                     /locus_tag="ACHKIK_000719"
     CDS             37986..40307
                     /gene="yhgF"
                     /locus_tag="ACHKIK_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO9081947.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVMSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQTAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            40745..40972
                     /gene="feoA"
                     /locus_tag="ACHKIK_000720"
     CDS             40745..40972
                     /gene="feoA"
                     /locus_tag="ACHKIK_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO9081948.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            40989..43310
                     /gene="feoB"
                     /locus_tag="ACHKIK_000721"
     CDS             40989..43310
                     /gene="feoB"
                     /locus_tag="ACHKIK_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO9081949.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            43310..43546
                     /gene="feoC"
                     /locus_tag="ACHKIK_000722"
     CDS             43310..43546
                     /gene="feoC"
                     /locus_tag="ACHKIK_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO9081950.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            43749..44627
                     /gene="rpnA"
                     /locus_tag="ACHKIK_000723"
     CDS             43749..44627
                     /gene="rpnA"
                     /locus_tag="ACHKIK_000723"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO9081951.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(44656..45426)
                     /gene="bioH"
                     /locus_tag="ACHKIK_000724"
     CDS             complement(44656..45426)
                     /gene="bioH"
                     /locus_tag="ACHKIK_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO9081952.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIVLTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            45464..46147
                     /gene="gntX"
                     /locus_tag="ACHKIK_000725"
     CDS             45464..46147
                     /gene="gntX"
                     /locus_tag="ACHKIK_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO9081953.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            46206..46781
                     /gene="nfuA"
                     /locus_tag="ACHKIK_000726"
     CDS             46206..46781
                     /gene="nfuA"
                     /locus_tag="ACHKIK_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO9081954.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            47142..48458
                     /gene="gntT"
                     /locus_tag="ACHKIK_000727"
     CDS             47142..48458
                     /gene="gntT"
                     /locus_tag="ACHKIK_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO9081955.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(48503..50587)
                     /gene="malQ"
                     /locus_tag="ACHKIK_000728"
     CDS             complement(48503..50587)
                     /gene="malQ"
                     /locus_tag="ACHKIK_000728"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO9081956.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(50597..52990)
                     /gene="malP"
                     /locus_tag="ACHKIK_000729"
     CDS             complement(50597..52990)
                     /gene="malP"
                     /locus_tag="ACHKIK_000729"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO9081957.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKMIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            53602..56307
                     /gene="malT"
                     /locus_tag="ACHKIK_000730"
     CDS             53602..56307
                     /gene="malT"
                     /locus_tag="ACHKIK_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO9081958.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(56350..57366)
                     /gene="rtcA"
                     /locus_tag="ACHKIK_000731"
     CDS             complement(56350..57366)
                     /gene="rtcA"
                     /locus_tag="ACHKIK_000731"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO9081959.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(57370..58596)
                     /gene="rtcB"
                     /locus_tag="ACHKIK_000732"
     CDS             complement(57370..58596)
                     /gene="rtcB"
                     /locus_tag="ACHKIK_000732"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO9081960.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT        13882 a        16166 c        14809 g        13766 t
ORIGIN      
        1 aacaaattta ttaaagttgc actggtaggt gcagtactgg ctacgttaac tgcatgtact
       61 ggtcatattg aaaaccgtga taagaactgc tcttacgact acctgctgca cccggcaatt
      121 tctatttcta aaatcattgg cggttgcggt cctactgcac agtaagtctc tgacagaaat
      181 cggctaacac cggtttaatt gcccgacgcc ctctggattt tccgagggcg tatttgtttc
      241 tatgtccttc ttgcccccgc ccgtaaaaat attttcactt taaattcaat ttgataacta
      301 catccattag ccacagcgcg atcctgtgcg aaattttgtg atcttcctcc acattacata
      361 acatcatatg ttgttatatt catcatgcat tgtcatgtta ccttttaaat gactgcaaac
      421 tctcccctac aacgtattgg acaagaaaaa ggtatcgcta tgggaagcca ggaactccaa
      481 cgcaagctcg gattttgggc cgttcttgca atcgccgtcg ggacaaccgt cggctccggt
      541 atttttgtat ctgtgggtga agtggcaaaa gcagcgggca cgccgtggct tacggtgctc
      601 gcgtttgtca ttggcgggtt aattgtgatc ccgcaaatgt gcgtctatgc ggaactatcc
      661 accgcttatc cggaaaatgg cgcagattat gtttatctga aaaatgccgg aagccgaccg
      721 ctggctttcc tctccggctg ggccagcttc tgggccaacg atgcgccgtc attgtcgatt
      781 atggcgctgg cgattgtcag caatcttggc tttttaacgc ctatcgatcc gttgctcggt
      841 aaatttatcg ccgccggatt aattatcgcc tttatgttgc tacacctgcg ctccgttgaa
      901 ggcggcgcag cgtttcagac gctaattacc atcgccaaaa ttatcccgtt cactatcgtc
      961 attggccttg ggatcttctg gtttaaagcg gagaattttg ccgcccctac caccactgcg
     1021 attggcgcaa cgggcagctt tatggcgctg ctggcgggga tctctgccac cagttggtcg
     1081 tataccggca tggcctctat ctgttatatg accggcgaaa ttaaaaaccc cggaaaaacc
     1141 atgccacgag cgctgattgg ttcctgtctg ctggttctgg tgctctacac cctgctggcg
     1201 ctggtgattt ccggcctgat gcccttcgac aaactcgcca attctgaaac gccgatttcc
     1261 gacgccctga cctggatccc cgcactcggc agcaccgctg ggatctttgt tgccatcacg
     1321 gcgatgatcg tcattcttgg ttcgctttcc agctgcgtga tgtaccagcc gcggctggaa
     1381 tacgcgatgg cgaaagacaa cctgttcttt aaatgcttcg gccatgtgca tccgaaatac
     1441 aacacgccgg atgtctccat catcctgcaa ggggcgctgg ggatcttctt catcttcgtt
     1501 tccgatctca ccagcctgct gggttatttc accctggtga tgtgtttcaa aaataccctc
     1561 accttcggct ccatcatctg gtgtcgtaaa cgcgacgatt acaaaccgct gtggcgtact
     1621 ccggctttcg ggctgatgac caccctcgcc attgcgtcaa gcctcattct ggtcgcctca
     1681 acctttgtct gggcaccgat tcccggcctt atctgcgccg tcatcgttat tgctactggt
     1741 ctgcctgctt acgccttctg ggcgaagcgt agccgccagc tcaacgcttt gtcgtaatgt
     1801 tatctggaga aaataaaatg ttggatattg ataaaagcac cgtggacttt ctggtcaccg
     1861 aaaatatggt tcaggaagtg gaaaaagttc tcagccatga cgttccgctg gtgcacacca
     1921 tcgtggaaga gatggtgaag cgcgacattg atcgtattta tttcgttgcc tgcggatcgc
     1981 cactcaacgc ggcgcaaacg gcgaaacatc tggcggatcg cttttccgat cttcaggtct
     2041 acgccatttc cggctgggag ttctgcgata acaccccgta tcgcctcgac gatcgttgcg
     2101 cggtgattgg cgtttctgac tacggtaaaa cggaagaggt aatcaaagcg ctggagctgg
     2161 gccgggcctg cggcgcactc actgcggcgt tcaccaaacg cgcggacagc ccgattacct
     2221 cggcggcgga atttagcatt gattatcagg ccgactgtat ctgggaaatt cacctgctgc
     2281 tctgctacag cgtggtgctg gagatgatca cccgcctcgc gccgaacgcg gaaatcggca
     2341 agatcaaaaa cgatctcaag cagttgccga atgcgctcgg tcaactggta cgcacctggg
     2401 aagaaaaagg ccgccagctt ggtgaactgg ccagccagtg gccgatgatt tataccgttg
     2461 ctgcgggtcc gctgcgtccg ctgggttaca aagaaggcat tgtaacgctg atggaattta
     2521 cctggacgca cggctgcgtg attgagagcg gagagttccg ccatggcccg ctggagattg
     2581 tcgaaccggg cgttccgttc ctgttcctgc tcggcaatga tgaaagtcgc cacaccaccg
     2641 aacgcgccat taactttgtt aaacagcgta ctgacaacgt gatcgtcatc gattacgccg
     2701 aaatttcgca agggctgcac ccgtggctgg caccgttcct gatgttcgtg ccaatggagt
     2761 ggctctgcta ctacctgtct atttacaaag atcacaaccc ggatgaacgc cgctattacg
     2821 gtggtctggt ggaatattaa tccctctccc ggcccggcaa cgggccgaat ttacgcaagg
     2881 agttacccga tgaaaacagg tatgtttacc tgcggccacc agcggctgcc tattgaacac
     2941 gcatttcgtg atgcaagcga gctgggttac gacggcatcg aaatttgggg cggtcgcccg
     3001 cacgcgttcg cgccggactt aaaagcgggc ggcatcaaac aaatcaaggc gctggcgcag
     3061 acgtatcaga tgccgattat cggctatacg ccagaaacca acggctatcc gtataacatg
     3121 atgctgggcg atgaacatat gcgtcgcgaa agcctcgaca tgatcaagct ggcgatggat
     3181 atggcaaaag agatgaacgc gggttatacg ctgatttccg cggcccacgc gggctatctc
     3241 acgccaccta atgttatctg gggacggctg gcagagaacc taagcgaact gtgtgagtac
     3301 gcggaaaaca tcggcatgga tctaatcctc gaaccgttaa cgccgtatga atcgaacgtc
     3361 gtgtgtaatg ctaatgatgt gcttcatgcg ctggcgctgg tgccttcgcc gcgcctgttc
     3421 agcatggtcg acatttgcgc gccgtatgtc caggcggaac cggtgatgag ttatttcgac
     3481 aaactgggcg ataaattacg tcatctgcat attgtcgaca gcgacggggc cagcgacacg
     3541 cattacattc ctggagaagg caaaatgccg ctgagggaac tgatgcgcga tattatcgac
     3601 cggggctatg agggttactg tacggtggag ctggtgacga tgtatatgaa cgagcccaga
     3661 ctctatgccc gccaggcgct ggaacgcttt cgcgcgctgc tgccggagga tgagagatga
     3721 aaaccctggc gacaatcggc gataactgcg tcgatatcta cccgcaactg aataaagcgt
     3781 tttctggcgg taatgcggtc aatgtggcgg tgtactgcac tcgctacggc atacagccgg
     3841 gatgcattac ctgggtgggt gacgatgact acggcacaaa gctgaagcag gatctcgccc
     3901 gcatgggcgt cgatatcagc catgtccata cgaaacacgg cgttaccgca caaactcagg
     3961 tggaactgca cgacaatgat cgcgtttttg gcgactacac cgaaggcgtg atggccgact
     4021 ttgccctgag tgaagaggat tacgcctggc tggcgcagta tgacattgtg cacgcggcaa
     4081 tctggggaca tgcggaagac gcattcccac agctgcacgc tgcgggcaaa cttaccgctt
     4141 tcgacttctc cgacaagtgg gacagctcgc tctggcagac actggtgccg catctcgatt
     4201 ttgcctttgc ctccgcaccg caagaagacg aagcgctgcg tctgaagatg aaagcgattg
     4261 ttgcccgtgg cgcaggcgtg gtgattgtca cgctgggtga aaacggcagc attgcctggg
     4321 atggcgcgca gttctggcgt caggctcctg aaccggtgac ggttatcgac accatgggtg
     4381 ccggagattc gttcattgcc ggattccttt gcggctggtc tgcggggatg acattaccgc
     4441 aggcgatggc gcagggaacg gcgtgcgcgg cgaaaaccat tcagtaccac ggtgcctggt
     4501 aggtataacg ttggcgtgag catcttcacg ccaacgtgct gttacttgcc ggaaaacgac
     4561 cctataatcc gagtaattca ttctttattt cagggtcgat tatgtcagct acggaccgct
     4621 actctcatca actcctctac gctaccgtcc gccagcggtt gctggatgat atcgcgcagg
     4681 gggtttacca ggccgggcaa cagatcccta ccgaaaacga gctttgtacg caatataacg
     4741 tcagccgcat tactattcgc aaagctatca gcgacttagt ggcagacggc gtactgatcc
     4801 gctggcaggg caaaggcacc tttgtacaaa gccagaaggt cgaaaacgcc ctgctgaccg
     4861 tcagtggttt taccgatttt ggcgtctcac aaggcaaggc gacgaaagag aaagtgatcg
     4921 agcaggaacg agtcagcgcc gcgccgtttt gcgaaaagct gaacatccct ggcaacagcg
     4981 aagtgttcca tctttgccgg gtgatgtatc tcgataaaga gccgctgttt attgatagtt
     5041 cctggatccc actgtcgcgt tatcctgact ttgatgagat ttacgttgaa ggaagctcca
     5101 cttatcagtt atttcaggag cgtttcgaca cgcgagtggt cagcgacaaa aagaccatcg
     5161 atatctttgc cgccacccgc ccgcaggcaa aatggctgaa atgcgaactg ggcgaaccgt
     5221 tgtttcgcat cagcaaaatc gcctttgatc agaatgacaa accggtgcac gtctccgaac
     5281 tcttctgccg cgccaatcgc atcaccttaa cgattgataa taaaagacat taaccgtagg
     5341 ccggataaga tgcgccagca tcgcatccgg cgatgctggc gcgttgaatt ttacatcccg
     5401 tacgttcccc tcaccctaac cctctcccca aaggggcgag gggaccgttc agtacggaat
     5461 ttagcttgac ggtgctatcc agcattaaat actggcaata ctctcacgca aaatccgcag
     5521 caccgtctct tcaccgctgc gattcgggtt aatgcgaatc gcacaatgtt ctgattgtgg
     5581 attcgcctgg cgaaacgttc cggaaaggcg ataaaagagc ggcgggattt catatttcga
     5641 ctctgcaccc accgggtaag gcaaggcacc gcgcttttgc gcctcttcca gcactctggc
     5701 ggcaatcggc tgatgaaact cgacaatcaa caccttcgac tgcgcattag caatcaccgc
     5761 gcttttcact tccggcaccg caccaccgtt aagcaaagcc agcaaccgtt cagatacccc
     5821 tgcctgcacc gcgtgcatca ctggcgcaaa caccagacca cgcaatactt ccagcgcctg
     5881 tgcgccctgg atctggctac cgccggagta aagcgtggcg cgaatacggt tgataacatc
     5941 agcatcgccg accactgcac caacaccctc tggcccaaat agcttgaagc aggaaaatgt
     6001 cgagacattc gcgccgcatt cacagccgat tcgcgccacc ttcatcaccg catagttgtc
     6061 atcggttaac gctggaacac ctgccgcgcg caacgttgcc agcacatctg ccagcacgta
     6121 gctgtcctgc ggctgctggc gcgtatgctg caccagcgcc gcatccggtt gttgctcgtc
     6181 gacgacctgc ttcagtgccg acaggtcatt gaaatcaaca gtaataagcg tcagccccat
     6241 ctgctcaata ataacccgtg tcgtcgggta aacaggcgcg tcatgcacca gaagacgctg
     6301 ccccggtttg agcaaagccg ccagcccggc gcgaatcgcg ccagtccccg cgccctgcac
     6361 cagcgccgca gcctgtgcgt gaaatgcatc agccagcacc tgctccaccc gttgggtaat
     6421 acgcggttga ttcagacctg gagttaaccc taaatcaccg ccagtaagaa actcgctgcc
     6481 ggggaaatgg cgacaaatgc tatccaccag cgcaaactgc ttttgctgcg cctcaataat
     6541 cgtcaggctt tgcagaggaa acgtcttcat aggtgccttc cttaagccgg aacgaacagg
     6601 ccaagccagt aaagaacatt cagcagaata ccggtgatca ttactgccac gaccggtgcc
     6661 gccattttct gtaccggacg gcccagggat tcgttgagga agtaaatggc aaccgcgata
     6721 gagaatccgg tataacccgc catcttaatt gctgcgaaaa tcgaaccgac cagcagcgcc
     6781 acttccatca gcatattcat ggcgttacgg atgttatccg acgcattacg caccgacggg
     6841 tagcgtccca gccatttgcc gatcgaacga agcagcaaga cttccgccga aataaccact
     6901 gcgcctaata ccgccgcaac catcggattt ggcgagagat agcccaccgc ataaacaaag
     6961 gtaaagcccg caactgcata cacgccagtg gctaacgcgg tggtggcaat caacggcaca
     7021 aaacccagtc cgcgcataaa ttctgccaga gccgcctgat taatcagcgt ttgcgattgt
     7081 tccggcgtca cgcctgcgga atacgctttc tccagtgtga agatcgaaac ttcgctacca
     7141 gcaaaaatct tcatactggc aacggcggca atcaatgctc ccacgatggc gatataaggt
     7201 aagtttttga taatccgcga cgtgcgctct tcaaacaccg aaagcccgct ggcatcgatg
     7261 tcattttcat cacgatgacg caggtcgtga gttatcgcga tccccagcag catcaccatg
     7321 ccaataaaga tttcgatgga ttcagggtta agatgtggga aatagcgcac gactaccaca
     7381 cgggtcatca gaacaaccac ggcggcgacc agactttgct tccagccaaa ctggtagaaa
     7441 atcgccacca gcgggaacag tgcaaaagca gaaaccaccg gcgagcttaa ttccccgagg
     7501 ctacctaata catccaccgg cagcgcggtc agcagctggt ttactggcaa caggcaagta
     7561 aggatcaaca cgccccagat agcgccaaga ccaaacgcca tcaggctgtt tatcgccagt
     7621 acgccgagga tatcggtagg aagaaagagt aaccatgcgt tgagcagccc ggttttcagg
     7681 gtaaaagaga tccccaccga ggccacaaaa ccaatgctca gaccaaaagc gatactcccc
     7741 gcctcgcgac gattcatata gccttcaatc agttgcggca ggatcgggcg gatgccgtca
     7801 tgaaaaacag ccgccgagcg atgcgccaga agcgatgtca tacccgtcag gcacgccacc
     7861 acgataatct gaatatacag atccatagtc gtgcctttta tttcagatgg ttaatcagca
     7921 tcggaatcgc gtgttcgacg tgctcaacgg aaaggccaaa cgccactttc ccttcagcga
     7981 tcattttggc gatatgctcg tctttcgctt taatgcctgg tttggcaatg gtgcaacttt
     8041 tgttatagcc aatcaccgcg atggcaatcg acaatgccgc gcccgcaccg gtattacacg
     8101 caccgatgta gtaatccagt tgcccggatt tcactttcat tgccgcttcc atgtcgttgt
     8161 gaatgaaaac ttcaaaactg ccaggagccg tcgcttcaat ggttttttta atctgctcac
     8221 gctgtaagcc tgcaacgcca atctttttca tgagtctgtc cttattggaa aaattgagag
     8281 ggattttcac gtaacatcac atccacatcg gcctgactga atcctgactg gcgcaattgc
     8341 ggaataaagg tggtcaataa atagtcatag ccataaccac cattggcttt taaatgggag
     8401 cggcgcgtaa tatccatcga cagcatgacg cggttcagca acccacggtc acgtagcgcg
     8461 tggagcatcg caatgcgctt ttcgtccggg tagtaactgt tcttaccaat ggtgtcgaac
     8521 tgcacgtacg cgccgagatc gatcatcttc aaaatgttgt cgagattgtc tttcagatcg
     8581 cagtgaccaa cggtgacgcg cgaaagatca actccgtggg cttgcaacag agccagttgt
     8641 tccagcccca tggtgctgaa cgaagtatgc gtggagatcg ggcgtccggt ctggttatgc
     8701 gccagcgcag cggcaataaa caccttctct tccagcggcg taatcttccc ttcgctagag
     8761 ccgatctccg cgataatccc ggctttcagg tcggtgccat cgataccctg ttcaatttca
     8821 tcgaccatct cctgcgccag ttcctgcacg ctgcgggtcg ccacatgctc cgggaaaaaa
     8881 gcgtcctggt aataaccggt gcaggccacc acgttgatcc ccgtctcgcg cattacatcg
     8941 agcataaatt gcgcattgcg ccccatgtaa cggttggtca tctcaatcac attacgcacg
     9001 ccccgggtca tcaggtcgtt catctcctgg caaatgaacg catactgatc aaggcggcag
     9061 tccacgttgt ttttaaagcc ggagagatca atatgcagat gctcatgggc cagggtgtaa
     9121 cccgtcggat caatactcat agggtgtctc ctgcaaaccg gagggaggaa agaaaagagc
     9181 gaccattttg tggcggtggc gcacggaaaa attcacacac ggtagccccc acatcagaaa
     9241 gcgtggtgcg tacaccgagt tgcgtgtgga ccagcccttg ctgataaacc agcactggca
     9301 ccacttcacg ggtatggtgg ctgtgaccaa tggtcggatc gttaccgtga tccgccatca
     9361 cgaccaggca atcatctggc tgcatcgcct caacaagccg ggcaaggtta cggtcaacga
     9421 cctgcaaacg ttcggcataa cgtgcgacgt cttctgcatg accagcgagg tcggtttcct
     9481 ggatattgat gcaaataaac gccgtcggat gcgtgttaaa ttcgtcgagg gtgatatcca
     9541 taatccgctg gctatccacc aggttttgcc agctcacgcc ataaggattg ctgacgatat
     9601 ctgccacctt acctaccagc acggttggca cgcccgcctc atacagtttt tgcggcacct
     9661 gcactttttc atcaacaccg tagcccatat gcacgacctg gaagccgttg tcataagcgc
     9721 cagaacgcgg cgcattaata ccaataaagc gcccttcttt gctttctgcg gcatcgagaa
     9781 tacgctgact gtcggttaac aagccgccaa atgtaatgac ccgaccgacc tgtacctgct
     9841 cacgcacgat acgaccaatt ttgatcgcgt cgtcaaaaga gatcacagag agattggcgg
     9901 taatgttata gacctggcct aaatccgcct cgagattatc gccaatcgca accgcctgat
     9961 tgacccacag aaattgcaga tcatcgccac ggcgctccac ctgccagcca gcggaaacta
    10021 atgcctgctc aacacggcca atcacatcgc gaaagggcat ccgcagcggc ggtaacgggc
    10081 gcgtgcctaa aatttcctga tgccccataa aggtatcgcc accttcatgt tgcagctctg
    10141 ccacccccca ggttgcggaa tctgacggtt gcatatcacc tggtgcataa cccaatgcgt
    10201 tgattagccc cagcttctcc agcgctggta gctgcaaatg cggcaactgg ctcaggatgt
    10261 gaccacaggt attcgccccg gcatcttgcg ggcgcaccag cgtgacatct ttcattgccc
    10321 ctacgccaaa gctatcaatc actaacacca caaatcgcgc cattacgccc ccagagagtt
    10381 tccaagactg tcataacgac cgacgatttt cggttcgcca cggtgaatac cggacaccag
    10441 caccacatcg ctacgagtga caaaaatctg cgtgcgaaaa cagagcacta ctgcactgct
    10501 taccggaaac tcgcctgcca gcggcagggt gtagtcgata ctgctgtcat ccacagtttt
    10561 gagattggtt tcagtaatct tttgattttc tggcgtaaac accagcgcat gttgcgcatg
    10621 accacgacgg tagtaaccgc cgccgtagca gtagctgtcg ccacggaaat gatgggagat
    10681 ttcacttaac caaagcatcg ctatatattc aggctgatcg ccctgctggt ttgccggaat
    10741 agtgcccgtc agcgcatgac cgggttcggc atgagtcaca ccgtattccg ccagcatcgg
    10801 cagcgaagtg cagctggtcg cagaaggcgc gttcagttgc tcaatcgcaa taccagattt
    10861 cgccagttga tcccgtgctt gtaccagcgt gtgaagattc ggtgtcggca aaactttccc
    10921 ggcagcctca tcccaaagca ggcaagggaa atgggtaagt ccggctaaat gcagacccgg
    10981 cagtttctta atttcagcaa ccacctcgtg cagcgaatgc tgtacaaaac cgctctcctg
    11041 ccccggataa agaaaatcat cgtcgctata aactttgagc agcacaaact gcacacgtcc
    11101 ggccttcacc gctgccgcag aaacttcccg cgctttgtcg agagtaaaca cggtgatgac
    11161 gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa gggatttgta ccagatgccc
    11221 ctgatgcgcc accgacaaac cagcgcggcg catgactcgc gcctctttgt aatccaccgc
    11281 cacaatgccg ctgtaaccta atgccagcaa tttttccgcc agccatgggt tacggccaaa
    11341 ctgcttggtc atcagataca gttcaatgcc gtaaagccgc gccgtctcaa tcagtcgctt
    11401 accgttttcc agtacctgat ccacgtcgat cacccagctg tccggggcga tcttgccctg
    11461 ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc tggcgtttca atgcttctac
    11521 aaacatcttg ttgcctcgtt tctgttcctt gaattaaata ttcatttttt tgaatattta
    11581 ggttgtgcga cgtggatttg tcgggagctt caaacctctt cgttagcagc catccataat
    11641 ccataaaggt tggccagcaa atagccttct tcacacgggt taacttccag tgcgaattcc
    11701 ttcaacaaca cctgatgcaa ttgcacaacg gcttgccagt ggctggattg cgccagctct
    11761 gccagcaatt cgttatccag cggctctatt tcttcaccac ggcgactgcg catcagtgca
    11821 ctcgccatat gtgtcatcgc catcgttcct tgctcactgc gcaccggcag atggcactct
    11881 gtttccagta cgttgacgac ctgcatcatg cctttgcaga tgtccttatc aataacgcct
    11941 gcctcgcaaa gcaggttgag tctggtttcc atatcagatg cctttcgttt aataactcgg
    12001 ttcctgttcc ccactcacta cccgttgttg atgggcgagc agggcgtgtt tatctacaac
    12061 ggcacgcagc agttgttgca ttgggtaatc atcgactcgc gtcagaacca cggcttcggt
    12121 ggcctgtaaa aatcgcggat cgtctgtgag cggcgtcgcc tctaatccca gcatggtcag
    12181 ttcgttttcc gccaccacgt tccagatcac cgcatcgaca tcgcctttaa caatgcgttg
    12241 taaactctcg tgataagaga gatcgacacg ttccacatca ctatcgccaa aaaagacatc
    12301 ggtcatgatt ttctgatccg ccgaacggtt atccagcccc acgcgcttca cgtttgcgga
    12361 ctcgccttta cggcaaatca actggtgctc gccaacgtag gtgtgcggcc ccaactccag
    12421 cgcgaggcaa aacccttttt gcgtgagata actttccgcc gccagtcggg aaaccaccgc
    12481 catgtcatac acgccgttaa gtaaacactc cacgcgaata tccgcgccgc gcatatgcgc
    12541 atagtaaaaa ggaatgccat caaactgggc tttcaatccg ctcgctaaac cttcgtacaa
    12601 gcgggtatag ggcaaaggca tcgcacatac cacgttgttg atatccacat gactcagcaa
    12661 tgctttgtta tccatctcga ccagataact gccattgcgc ccacggcgtt caatccgtat
    12721 cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc aggccaacgg atgaacggca
    12781 ttcagttgcc agctcatcta tggttttcaa cctattaccg catttttcac ccaacaaata
    12841 acgggccagc gtcgtgataa cgacgccttc ttttttgata aacgttcgac gcataatata
    12901 ttttcaataa aatgaatgtt tatatcttca ttaaaatgaa gataagtggc aactgtgaga
    12961 tgtggaaacg gcgaggcgct tcacattttt tcggattatt gcaaatgagg ataaaaaaac
    13021 cgggtttccc cggtttcaga gtgatgacga agtgatttat tcgacaatgc gagcaaaggg
    13081 taattcccac tggcgcttgc ctgctcccct caccctaacc ctctccccaa aggggcgagg
    13141 ggaccgtccg agcgttttga tattacggct tcgccacaaa accaatcgct tcgtacaccg
    13201 ctttcagcgt acgggaagcg tgtacactgg ctttttctgc gccgtctttc atcacctgtt
    13261 gcaggaaggc ttcatcgttg cggaaacggt gatagcgttc ctgcaattca gtcagcatac
    13321 cggaaacggc atcagccact tcacctttca gatgaccata catcttgcct tcgaactgtt
    13381 tttccagttc tggaatgctc tggcccgtta ccgcagaaag gatatccagc aggttggaaa
    13441 cgcccgcttt gttctgcaca tcgtagcgaa ctaccggcgg ctcgtcggag tcagtgaccg
    13501 cgcgtttgat tttcttcact accgatttcg gatcttccag caggccgata acgttattgc
    13561 ggttatcgtc agacttggac atcttcttgg tcggctccag cagcgacatt acgcgcgcgc
    13621 cagatttcgg aataaacggc tccggcacct taaagatctc gccatacagc gcgttgaaac
    13681 gctgggcgat atcgcgactc agttccaggt gctgtttctg gtcttcaccc accggtacca
    13741 gattagtttg atacagcagg atgtccgcag ccatcagcac cggatagtca aacagaccag
    13801 cgttgatgtt ctcggcataa cgcgcagatt tatctttaaa ctgcgtcatg cggctcagtt
    13861 cgccgaagta ggtatagcag ttcagtgccc agcctaactg cgcatgttct ggtacgtggg
    13921 actgaacaaa aatggtgctt ttctcaggat cgataccaca agccagatac aaggccagcg
    13981 tatccaacgt cgctttacgc agcttctgtg catcctggcg cacggtgatc gcgtgttggt
    14041 caacgataca gtaaatgcaa tggtagtcat cctgcatgtt tacccactga cgcagcgcac
    14101 ccatgtagtt accaatggtc aattcacctg agggctgtgc gccactaaaa acgatgggct
    14161 tagtcatttt tcgattcctg attttcgcta tgcggaagcc ctaatgcggg cagaaggtca
    14221 tttatagact gataaattac atcaggctgg ctgagatcga tagcctcgcc gtagttatat
    14281 ccgtaggtta agccaactga tgggcaacct gccgcttttg ccgcctgaat atcattgcgt
    14341 gagtcgccga caaacagcat ctgttgtggg gcaattccca tccgctcagc caccagtaac
    14401 agcgggtccg gatgcggttt tttgttttgc acatcatcgc cgccgataac cacgctaaag
    14461 tatttggcaa tatctaaggc ttcgagcagc ggcgcgacga acggcgtcgg tttgttggtg
    14521 accaggccta gcggcaggcc tttagcctgc aacgcgccca acgtatcggc aacgtgcggg
    14581 aacaaaaacg tcccctcttc ggcaacctcg ccatagtagc gatcgaacag tttacgcaga
    14641 atacgtacct gttcttctgc cggaatgtcg tcatcaacgg gcggtttacc cattgtttta
    14701 cgctgagtcg cacgttcctg acgcgcccag gtcaatgcgc gctccatcag aacatctgcg
    14761 ccgttaccaa tccaggtaat aacgcgttct tcacctgcga cgggcaactc cagcgcatac
    14821 agcgccatat ctaccgcagc agcaagaccg ggagcactgt cgaccagcgt accatcaaga
    14881 tcaaaagcga cgccgcgaat atcttcaaac ttattcatga cttaactttg ccagttcact
    14941 gcgcatttca tcaatgactt ttttgtagtc tggctggtcg aagattgccg aaccggcgac
    15001 gaacatatcc gcgcccgccg cagcgatttc gccaatgttg ttcaccttca cgccaccgtc
    15061 tacttccaga cgaatgtcaa agccagactc gtcgatacga cgacgtactt cgcgcagttt
    15121 atccagtgtt tgaggaatga acgactgacc gccgaaacca ggattgacgg acatcagcag
    15181 gatcacatcc agcttatcca tcacgtaatc cagatagctc agcggtgtcg ccgggttaaa
    15241 taccagtccc gctttacagc cattttcttt aatcagttgc agcgtgcggt caacatgctc
    15301 ggaggcttct ggatgaaagg taatgatgct ggcaccagcg gcagcgaaat caggcacaat
    15361 gcgatcgaca ggtttcacca tcaggtgtac gtcgataggg gcggtaatgc cgtagttacg
    15421 caaggatttc agcaccattg gcccaatcgt cagattggga acatagtggt tatccatgac
    15481 gtcaaaatgc acgacatcag cgccagctgc cagggctttt gcggtatctt cacccaggcg
    15541 ggcaaaatca gccgacagaa ttgagggggc aatcaaatac tgtttcatcc gcttctcctt
    15601 gagaattatt ttttcgcggg tgaaacgact cctggtttgt acaaagccag cagttcgtcc
    15661 acctttttac gtgtgccgcc gttgctgctt atactgcgtc gaactttgac gacatgcaat
    15721 tttgcgcgct gataccactc acgcgttaac atcgtatcgt gattggagat cagcactgga
    15781 atatggcgct caaccagacc ttcggcgatc tccgccagat gcgcttgttg ttcaagcgta
    15841 aaactgtttg tgtgatacgc cgtaaagttg gcggtcgcag acagcggtgc ataaggcgga
    15901 tcgcaataga cgacggatgc atcatctgcg cgcgccatgc tatcggcgta agactcacaa
    15961 tagaaaaagg cattctgcgc tttttcagcg aagtgataca actctgcttc cgggaaatag
    16021 ggttttttgt agcggccaaa cggcacgtta aactcaccgc gcagattgta acgacacagg
    16081 ccgttgtaac cgtagcggtt caaatataaa aacagtaccg cccgacggaa cggatcctgg
    16141 cttttgttga actcttcgcg gaactgatag tagacctcgg cgcaatttgt ttcgggaaca
    16201 aacagctcgc gtgcggcctg tacgtactca tcagtacgca tcttcacaat gttatagaga
    16261 ctgatcaggt cgctattgat atcggcaagg atataacgag aaaagtcggt gttgagaaac
    16321 accgacccgg cacctacaaa aggctcaacc agacattcgc ccttgggcaa atgccgttta
    16381 atatcatcaa gcaggggata cttgccccct gcccacttca aaaaagcgcg atttttcttc
    16441 atgctgacta actaattaca ccttctccgg ctgtggagaa agctccgaca gcatccagcg
    16501 cttcaggcaa ctcccgcaac gataacccat tgcgggagat aaccttgatt acttcagatc
    16561 ggcctgtacc tgacgcagcg gtttcgccca cgggtttttg gcctgaacat ctgctggcaa
    16621 tgtagatacc gcttttttcg cctcttcttt cgaagcgtac acgccagaaa ccaggacata
    16681 ccacggctga ccattacgcg tcgtttcata gacaacgtag tttttcagat tctctttctt
    16741 cgcccaaccg ttcaggttgt cgtagttaga ggaactgctc agctgcagag tgtaatggct
    16801 ggacggtgcc gatttcaacg aaccaacatt acctgcggtc ttccctccag cggctggtgt
    16861 tgccgtggtt tgcgccgggg atgccgtctg tactggagcc gtagtcgccg tctcttttgg
    16921 tgctggcgca gaagtcgcag ccggtgcctt cgtgctcgcc acaggagcag ctggttcagt
    16981 acgcttcggc gtctgtgcta ccggcttcgg ttccgttttc acggttgctt gcggtttttt
    17041 cggttcaatc accgcctgct gacgagctgg acgcgtagtg gccggacgtt cagcagtttg
    17101 cgttttcgcc gtgtcacgcg atgcattgcc attgcgtact ggcgcgacag ttgctggttc
    17161 agtcggcaat gtagaattga ccgccacatt gttcaactgt tgctgatttt gtggctgggt
    17221 cagcgcattg ttcaggtcac cctgcacttc aacacgttgt tgaccatccg ttgccgccgg
    17281 ggtttgccct tgagtcggcg tagaagagat cggtggcaga gaaacatcct gctgagtatt
    17341 ttccgcagac gtggttcccg gcgctggctg caccccattc gcctgatcgg tcgcattgcc
    17401 tgcaagatca atactcttct cgccagacgc ggtttgatcg ctggaagagg tcgagggggc
    17461 ttttagcgca gaaccgatac cgatgatcaa cagcagtaga accagaatgc cgacgcccat
    17521 catcatatac tgacgagaag cgggtttact ggctgctttt ttgcgcttac gcggacgacg
    17581 ctctacgcgc tcttcatcca cggtttcatc ttcggattcg tcaatttctt cttcgatttc
    17641 cggttcctca ttgcgctctt tttgcgcacg agtcggacgg cgatcgtcag tgtcatcaag
    17701 ttcaatatca tcaaaattga tctgcggttc gccacgttca gtacgctcag aacgttcaga
    17761 agattgacga gaacgaccag tacgacgatc gctgggatcg ggtttcagct cgtcttctgg
    17821 tttgaattca tccatttaac accccactaa aaggttaatg cttaccacat tgcaattaac
    17881 ctgaagctaa tagaccgctt gataagcggc ctgacctttc ttgttgttac gctgattgac
    17941 tatcggcaat ggcgttaaga acaagctcgt gcgaaacgcc gctgcgaact tcactcttac
    18001 caattgccaa cggaagaatt aagcgtatct ctcccgcaag gactttcttg tcacgcagca
    18061 tatgcggtaa atacgcctgc gcggacattt cgcgcggccc attgaccggt aacccagccc
    18121 gcttgagcag ggttataata cgctgcgttt cggcagaact aaactgcccg agacgttccg
    18181 acgtccgcgc cgccatcacc atacccgcag cgaccgcttc accatgtaac caattgccat
    18241 accccatttc agcttcaatg gcatgaccaa aggtgtgtcc cagattcagt aaagcacgta
    18301 acccggtttc gcgctcgtcg gcggcgacaa cttctgcctt cagttcacaa caacggcgaa
    18361 tacagtacgc cattgccgga ccgtccagac gcaacaacgc atccagattc tcttccagcc
    18421 agttaaaaaa cgcaccgtca agaataatgc cgtatttgat gacttctgcc agccccgacg
    18481 ctaactcacg cgggggaagc gttttcagac agtcgagatc caccaccact gaagcaggct
    18541 ggtagaacgc gccaatcatg tttttaccga ggggatggtt gaccgcagtt ttgccgccaa
    18601 cggaggaatc gacctgcgac agtaacgtcg tcgggacttg aatgaaacga acaccgcgct
    18661 gataactcgc cgccgcgaag ccggtcagat cgcccactac gccgccgcca agcgccacca
    18721 gcgtagtatc gcgaccatgc ggcttttgta acaacgccgt aaagacggta tcgagtacag
    18781 ccaggctttt atactgctcg ccgtcaggga ggataacgct atcgacgtta acacccgcct
    18841 gttcaagtac gccgcggacc ttatcgagat acagaggagc cagggtttcg ttggtgacca
    18901 acatgacctg ctcgcccgat ttcagcggta agaatgaagc tggttcatta aacaaaccag
    18961 atgcgatggt aattgggtaa ctacgttccc cgagagtaac gacaatcctc tccataacgc
    19021 gacatccacc ttaattactg tacccgcaga cgagtgtata taaagccaga attagttgct
    19081 ttccagcatg tgaataatct ggtttgcaac cactttagcg ctttgatcat cagtacgaat
    19141 ggtcacgtcg gcaatctctt catacagcgg attgcgttca ttggccaacg cttccagaac
    19201 ttcacgcggc ggtgtttcaa cgtgcagcaa cgggcgtttt ttatcacgct gcgtgcgtgc
    19261 aagttgcttt tcgatggtcg tttcaagata aacgacaacg ccacgagcgg aaagacggtt
    19321 acgcgtttca cgggatttca cagagccgcc gccagtagcc agcacaatac cctgtttctc
    19381 ggtcaactca ttgatgacct tttcttcgcg atcgcggaag ccttcttcgc cttctaaatc
    19441 gaaaacccag cccacatcag ctccggttcg tttctcaatc tcttgatcgg aatcgtaaaa
    19501 ttccatattg agttgttgag ctaactggcg cccaatagtg ctttttccgg cacccatagg
    19561 cccaaccaga aagatattgc gtttctctgc cattttttcg gtactactaa gactattcgt
    19621 taatgataaa cccgcttcgc tcagagagcg ccgcaggaca tgaactgaaa cctcataaga
    19681 tattgcgaga gtcagactga aaattatctc aatactccag cgggtttggc aactgaataa
    19741 atcacccatc cctctgcata tctggtcgct gcaagcgcgc tgccttgcta ccaccgctct
    19801 ggcgataaat caccgggtaa gattagcgta aaaaagacag caaaatgccg cctgaatgat
    19861 aaatcatcat catggaacgc aggcagcgaa gagcaccaac tctcaaatcg gtactccttg
    19921 tatgctaaat acctgcgcgc gtcaaataga tgaaacacgt tcagcataaa aacattaccg
    19981 cttttacggc tgtttactca ctggaaacca gtcgtggcgt gataaacacc actaactcgc
    20041 gtcgttcatc ttcttttccg tcatgacgaa ataattgccc gaaccaggga atgtcgccaa
    20101 gcaacggtac gctatcctga cccgatttat ttttacgggt aaaaatgccg cccagcgcca
    20161 acgtttctcc gcttttgacc tcgacctgcg tttcgatctc ctgcttatca atcgccagca
    20221 cttcgccatc ggcctgctgt agcacctgcc ccggaacgtt ctggctgatg tgtaatttca
    20281 gccggatgcg acctttttgt aacaccgtgg gcgtgacctc catccccagg acggcctctt
    20341 taaattccac cgacgtcgcg ccactttccc cgctggaaac ctgatatgga atttcgctcc
    20401 cctgtttaat gctggcaggc tgaagatgtg aggccagcag acgcggactg gcgataatat
    20461 ccagctgctg tttttgttcg agcgcagaaa gctcaagatc cagcaagcgt ccgttgatgc
    20521 gcccaatgtt aaaaccgaca tgcgttgtcg ccgtcgctac ggagaggtcg ctaccaagcg
    20581 tggtgacttg cccaacgcca ccagcgtgtt gcgcatcggc cagcgtccat ttcacgccta
    20641 actcacgcaa acttttttca ttaatggtga caatatgcgc cgacagctca acctgcccga
    20701 ccggcagatc catttgcgct acccactgtt caagcgcgct taacgccgtt ttgttatctc
    20761 gtagcaaaag gcgattggtg cgtttatcga cggtcatact ccctttggca ctcagtagct
    20821 tctcccccgc tttcgccagt tctcccgcgt cggcgtattg cagggttata ctgcgatttt
    20881 ccagcggcag atttgcctgc gcccgcgcct gctccgcctc ctggcgggcg atattgttat
    20941 tctgccaggc aatggaatgc actgagagaa tgttgtcttc ctgccgcgtt atcagtccgg
    21001 cgcttttcac tacagtttgt agtgcctgct tccagggaac atctgttaga tgtaacgaca
    21061 ccgtaccgct gacgtctggc gacacgacca ggttcaactt ctcctgttca gccagcgcct
    21121 gcaacacctg agctaccgga acgtcatcca ccatcagcgt cactttttgc ggctttgccg
    21181 cctgtacgcc gggtatcagc atcaacagta gtgcggctat ccattgcttc atttgtatct
    21241 ccttgccgtt gccacaacca ttgtggcggt tcgcagtttg tcccggttcc cagcgttagt
    21301 gcgtctggcg tcagctgtaa aattgtccag ccgttttcca gcacatcgtt ttgctgcacc
    21361 cgtcgccatt tcttttgccc gtcttttatt acaccgatga tgtgctcgcc tcgccctacc
    21421 atcccctgat agcgccactg gctaagttcg ctaatccggc atagatcttc cggcggttta
    21481 aaagggtcac gcataccggt taaaaggcac aatgcaatac ctgccaacaa ccagcgttta
    21541 accctcatgc ggtgtctcca gttgtagcgt gaacaaaaga tcatcacctt ccacgcttaa
    21601 cgaaaaacgg ctcacgctga cgttgcgctc tgccagccgt gtaaatgccg acggcactgc
    21661 ttcccacagc gttttcaacg ccaactcacc tccctgcgcg gatggatgcc aggaaaccag
    21721 ttgcgcgccg ggcaactgaa aatccagtgg cgaaaagggc agcgtttttt cttcgctgaa
    21781 gggagtggtg tcaaccaggc gatacagtgt ggcccactgc tggtgatgac ttgcccgtag
    21841 tcgaattaat gcgtcgcgct cttcatggtg tgtcgatgac agaaaaatga gcgtaactaa
    21901 catcagcaac cagaatgccc agcaaaactg gcggaggcgg ggtgatgtgg cgaaccacca
    21961 gtcaaagaac atgttcatcg ctaaccttcc ttgttaactg atactcaaat tgccagcgtc
    22021 cctgcgcatc ctgctgcgtg gctccccgct gattgagatg aaaagacgca tcctggcgga
    22081 gtgacgtttc tagtgcgttt aacgcggtaa tgcttgttgt cagccccttg atctccagcg
    22141 ttccctgctg ccagcttatc gttgtcagcc aggcgtgctc gggtaaaagc gccgccagtg
    22201 cttccagcgc agattgccag tcgcgggtaa attgtcgctg gcgctgccgt tgcgaacgct
    22261 gttcgcgtaa ctgctgttgc tccagcaaac gtggcttcgt tatctgtaag ctgcgggcga
    22321 gttgttgttc cgcctgaagc aaaactgcgt ctatgcgcgc ttcggcgctg cctgtcagac
    22381 gcagtattag cgttatcccg acggccagca gcagaggcgc aacgaacatc agcaaccaga
    22441 aacgcagaaa agcggtccgg cgttgctgtc gccagggcaa aaaattaatt ggcgggttca
    22501 tcagtacgcc ttcccaagcg ccagccccag cgcgatggca aagtctccac caggcggcgg
    22561 tagcggcggc tgacgaacag aaacggcctc ccaggggtca aatccgcctt cgccacatat
    22621 cgcgacgctg tcgggatcaa cggataacgc tgccgccagc tccttcgcgc tagtcatccc
    22681 caccgccagt ttgcgccccc agctatagcg cgtcgcccac agccactgtt cgttatcacg
    22741 ccaggccaga cattgctgat gagaaggtaa aaaaggcagg aatcgctgta atgcactggc
    22801 atccggggtg atcgcactaa catgaacacg caacctttct gccagagtaa gcagcgttgc
    22861 cagctctttg ctttgcgcgg cagtcacgtt ataggcggga ctgagtgagt cttcgctgta
    22921 atcgaaacgc agggagtccg gatccatatc cagctcgcgg gccatcgtcc ctgacagcca
    22981 ggccgtttgc tcccgctcac caagggacat cgacgggcgc ggaaatgacc gctgtaatgt
    23041 gcgactggcg ggaaacgcca acataatgtg atgacgctgc ggcagttcgc gactccacgg
    23101 taacaacgtt ttagccagct gctgcgcatc aacaatccgc ccatctttga taatgtcgtt
    23161 ctccagcggc aaccgccacc agcgttgcaa aaagcattct tttgctcccc gcacgatcgc
    23221 aaccgctacc gcttcttgct gttgtaaatg caaaccaatt tgccagatct taaatgccat
    23281 tgtgatgctc tccttatcac ccgtcactct gacgggtata tcaatgcgtc tggcttgcct
    23341 ttatactacc gcgcgtttgt ttataaactg cccaaatgaa actaaatggg aaatttccag
    23401 tgaagttcgt aaagtatttt ttgatccttg cagtctgttg cattctgctg ggagcaggct
    23461 cgatttatgg cctataccgc tacatcgagc cacaactgcc ggatgtggcg acattaaaag
    23521 atgttcgcct gcaaattccg atgcagattt acagcgccga tggcgagctg attgctcaat
    23581 acggtgagaa acgtcgtatt ccggttacgt tggatcaaat cccaccggag atggtgaaag
    23641 cctttatcgc gacggaagac agccgcttct acgagcatca cggcgttgac ccggtgggga
    23701 tcttccgtgc ggcaagtgtg gcgctgttct ccggccacgc gtcacaaggg gcgagtacca
    23761 ttacccagca gctggcgaga aatttcttcc tcagtccaga acgcacgctg atgcgtaaga
    23821 ttaaggaagt cttcctcgcg attcgcattg aacagttgct gacgaaagac gagatcctcg
    23881 agctttatct gaacaagatt tacctcggtt accgcgccta tggtgtcggt gctgcggcac
    23941 aagtctattt cggtaaaacg gtcgaccaac tgacgctgaa cgaaatggct gtgatcgccg
    24001 ggctgccgaa agcgccttcc acctttaatc cgctctactc aatggatcgt gccgtcgcgc
    24061 ggcgtaacgt cgtactgtcg cggatgctgg atgaagggta tatcacccaa caacagttcg
    24121 atcagacacg cactgaggcg attaacgcta actatcacgc gccggagatt gctttctctg
    24181 cgccgtacct gagcgaaatg gtgcgccagg agatgtataa ccgttatggc gaaagtgcct
    24241 atgaagacgg ttatcgcatt tacaccacca tcacccgcaa agtgcagcag gccgcacagc
    24301 aggcggtacg taataacgtg ctggactacg acatgcgcca cggctatcgc ggcccggcaa
    24361 atgtgctgtg gaaagtgggc gagtcggcgt gggataacaa caagattacc gatacgctga
    24421 aggcgctgcc aacctatggt ccgctgctgc ctgccgcagt caccagcgcc aatcctcagc
    24481 aagcgacggc gatgctggcg gacgggtcga ccgtcgcatt gagtatggaa ggcgttcgct
    24541 gggcgcgtcc ttaccgttcg gatactcagc aaggaccgac gccgcgtaaa gtgaccgatg
    24601 ttctgcaaac gggtcagcaa atctgggttc gtcaggttgg cgatgcatgg tggctggcac
    24661 aagtgccgga agtgaactcg gcgctggtgt cgatcaatcc gcaaaacggt gccgttatgg
    24721 cgctggtcgg tggctttgat ttcaatcaga gcaagtttaa ccgcgccacc caggcactgc
    24781 gtcaggtggg ttccaacatc aaaccgttcc tctacaccgc ggcgatggat aaaggtctga
    24841 cgctggcaag tatgttgaac gatgtgccaa tttctcgctg ggatgcaggt gccggttctg
    24901 actggcagcc gaagaactca ccaccgcagt atgctggtcc aattcgctta cgtcaggggc
    24961 tgggtcagtc gaaaaacgtg gtgatggtac gcgcaatgcg ggcgatgggc gtcgactacg
    25021 ctgcagaata tctgcaacgc ttcggcttcc cggcgcaaaa cattgtccac accgaatcgc
    25081 tggcgctggg ttcagcgtcc ttcaccccaa tgcaggtggc gcgcggctac gcggtcatgg
    25141 cgaacggtgg cttcctggtg gacccgtggt ttatcagcaa aattgaaaac gatcagggcg
    25201 gcgtgatttt cgaagcgaaa ccgaaagtag cctgcccgga atgcgatatt ccggtgattt
    25261 acggtgatac gcagaaatcg aacgtgctgg aaaataacga tgttgaagat gtcgctatct
    25321 cccgcgagca gcagaatgtt tctgtaccaa tgccgcagct ggagcaggca aatcaggcgt
    25381 tagtggcgaa gactggcgcg caggagtacg caccgcacgt catcaacact ccgctggcat
    25441 tcctgattaa gagtgctttg aacaccaata tctttggtga accaggctgg cagggtaccg
    25501 gctggcgtgc aggtcgtgat ttgcagcgtc gcgatatcgg cgggaaaacc ggtaccacta
    25561 acagttcgaa agatgcgtgg ttctcgggtt acggtccggg cgttgtgacc tcggtctgga
    25621 ttggctttga tgatcaccgt cgtaatctcg gtcatacaac ggcttccgga gcgattaaag
    25681 atcagatctc aggttacgaa ggcggtgcca agagtgccca gcctgcatgg gacgcttata
    25741 tgaaagccgt tcttgaaggt gtgccggagc agccgctgac gccgcctccg ggtattgtga
    25801 cggtgaatat cgatcgcagc accgggcagt tagctaatgg tggcaacagc cgcgaagagt
    25861 atttcatcga aggtacgcag ccgacacaac aggcggtgca cgaggtggga acgaccatta
    25921 tcgataatgg tgaggcacag gaattgttct gattaaaaag gcgcttcggc gccttttcag
    25981 ttggctgaca aagtgcattt gtttatgccg gatgcgggtg aacgcgttat ccggctaaca
    26041 aaatcgtgaa aaaatcaata tattgcagga aacccataag cctgataagc gttgtgcatc
    26101 aggcaaactt cacgcattta cactcgcccc tgccctttca accattcgcg cacgaggaac
    26161 agcgcgctga cattacgcgc ttcgttgaag tcagggtctt ccagcaaatc catcatatgc
    26221 gccagcggcc agcgcacctg tggtagcggc tctggctcat cgccttccag cgattccggg
    26281 tagagatctt gcgcgaccac gatattcatt ttgctggaaa agtaagacgg tgccatgctg
    26341 agcttcttca aaaaagtcag atcgttcgca ccaaatccaa cctcttcttt tagctcgcgg
    26401 ttagcggctt cgtagacgct ttcacccgga tcaattaatc ctttcgaaaa acctaattcg
    26461 taggattcag ttcccactgc gtattcgcgg atcaggatca ggtgatcgtc cacaatcggc
    26521 acaatcatca ctgcttcccg gttggttgga cgcattcgtt cataaacacg ccgcacgcca
    26581 ttgctgaact ccagatccac gctctcgacg gtaaacagtc gggaacgggc tacagtttca
    26641 acattcagaa tggtgggttt ttgtaatgat ttgctcatcg tgggatctat gctgtgaaat
    26701 cagcggttat tgtgcgatat cggacacgct ttcggcaatg tgaattgcat gttatttaca
    26761 tttatgtaac ttaataaata atcgtcctca aatcaaatta aaagtcaata ggttgaaata
    26821 actccaggaa tttgctgata ttccgccttc agagggtttg ctatgatcag cggttactgt
    26881 gatgtgctta atgatgctca agttaaactc cacgcttgcc gatagccaac cgcagaatca
    26941 tgtattgtgt ctggtgcgac tgaccacgcc tgacagacta agtaagatgg ggaaagcatg
    27001 agcaccattg tgattttttt agctgctttg ctggcctgct cactacttgc gggatggctg
    27061 ataaaagtgc gatccagacg gcgtcagctg ccctggacca acgccttcgc ggatgcgcaa
    27121 acgcgtaaac tcacacctga agaacgtagc gccgttgaaa attatcttga gagcctgacg
    27181 caggtattac aggtgcctgg cccaacggga gccagcgcgg caccgatctc tctggcgctg
    27241 aatgccgaaa gcaacaacgt catgatgctg acacacgcta tcacgcgtta cggcatctct
    27301 accgacgatc cgaataaatg gcgttactac ctcgattcgg tagaagtcca cctgccccct
    27361 ttctgggaac aatacatcaa cgatgagaat accgttgaac tgatttatac cgattcgctg
    27421 ccgctggtta tttcactcaa cggtcatacg ctgcaggagt acatgcagga aacgcgcggc
    27481 tatgccttgc aacctgttcc gtccacgcag gcgtcgattc gcggggaaga gagtgagcaa
    27541 atcgagctac tcaatattcg caaagaaacg catgaagaat atgcgctgag tcgtccgcgc
    27601 gggctgcgtg aagcgttgct gatcgtcgcc tccttcctga tgttcttttt ctgcctgatt
    27661 accccggatg tatttgttcc gtggctggca ggcggcgcat tactgctgct gggcgcaggt
    27721 ctgtgggggc tattcgcgcc cccggcaaaa tcctccctgc gagaaattca ttgtctgcgc
    27781 ggtacacccc gtcgttgggg attgtttggc gaaaacgatc aggaacagat caacaatatt
    27841 tcactcggta ttatcgacct ggtctatccc gcacactggc agccatacat tgctcaggat
    27901 ctcggtcaac aaaccgatat tgatatctat ctcgaccgcc acgtagtgcg tcaggggcga
    27961 tatctttcgc tgcatgatga agtaaaaaac tttccgttac agcactggct gcgcagtacg
    28021 attatcgctg cgggctcgct gctggtgctg tttatgctgt tattctgggt cccgctggat
    28081 atgccgctga aattcactct ctcatggatg aaaggcgcgc agaccattga agccaccagc
    28141 gtaaaacaac tggctgacgc tggcgtgcgg gtaggcgata cattgcgtat tagcggtacg
    28201 ggaatgtgta atattcgcac ttccggaacc tggagcgcga aaaccaattc acctttttta
    28261 ccgtttgact gctcgcagat catctggaat gacgcccgct cattaccatt accagaatct
    28321 gaactggtca acaaagcgac ggcattgact gaagcagtta atcgccagct gcaccctaaa
    28381 ccggaagatg aatctcgcgt cagtgcctca ttacgttcag cgattcaaaa atccggcatg
    28441 gtattgcttg atgattttgg cgacattgta ctgaagacag cggatttatg ttctgccaaa
    28501 gatgactgtg tgcgactgaa aaatgcgctg gtcaatctcg gcaacagtaa agactgggac
    28561 gcgctggtaa aacgcgccaa cgccgggaag ctcgatggcg tgaatgtgtt attacgcccg
    28621 gtgagtgcag aatcgctgga taacctggtg gcaacctcca ccgcgccgtt tatcacgcat
    28681 gaaacggcgc gagcggcaca atcactaaac agtccggccc ccggcggatt cctgattgtc
    28741 agcgacgaag gcagcgattt tgttgatcag ccctggcctt cggcatcact ttacgactac
    28801 ccgccgcaag aacagtggaa cgctttccag aaactggcac aaatgctgat gcatacgccg
    28861 tttaacgccg aaggtatcgt cacaaaaatc ttcactgacg ccaatggtac gcagcatatt
    28921 ggccttcatc cgatcccgga tcgttccgga ctgtggcgct atctcagcac cacattgctg
    28981 ctactgacga tgctgggtag cgccatttac aatggcgtac aggcctggcg tcgttaccag
    29041 cgtcatcgca ctcgcatgat ggagattcag gcctattatg aaagctgcct gaacccgcaa
    29101 ctgatcaccc cttcagaaag ccttatcgaa taacacgttt gcgcggcagg ttatgctacc
    29161 ctgtcgcgca aattgcttca ctctggagat ttccctcatg catatcaaca ttgcctggca
    29221 ggacgtagat accgttctgc tggatatgga cggcacgttg ctcgacctcg ccttcgataa
    29281 ctatttctgg caaaagctgg tgcctgaaac atggggcgcc aaaaacgggg ttacgccaca
    29341 ggaagcgatg gaatatatgc gccagcaata tcacgacgta cagcatacgc taaactggta
    29401 ctgtcttgat tactggagtg agcaactggg tctggatatc tgtgcgatga ccaccgagat
    29461 gggaccgcgt gccgtactgc gtgaagatac cattccgttt cttgaggcac tgaaagccag
    29521 cggtaagcag cgaattttgc tcaccaatgc gcatccgcac aacctggcgg taaaacttga
    29581 gcataccggt ctggacgcac accttgattt attactttcc acccacacat ttggttatcc
    29641 gaaagaggat cagcggttat ggcatgcggt ggccgaagct acgggtctga aagctgaaag
    29701 aacgctgttt attgatgaca gcgaagcgat tctcgatgct gccgcgcaat ttggtattcg
    29761 ttactgcctc ggcgtgacta atcctgattc cgggattgcc gagaaacagt atcaacgcca
    29821 tccgtcactg aatgactacc gccgcctgat cccctcgcta atgtgaagga gacgccatga
    29881 aagagaaacc tgctgttgag gttcgactgg ataaatggct atgggctgcc cgtttttata
    29941 aaacccgcgc gctggcccgt gaaatgattg aaggcggtaa ggtgcattac aacgggcagc
    30001 gcagcaagcc gagcaaaatc gtcgagctga atgccacgct cactctgcgc cagggaaatg
    30061 acgaacgcac ggtgattgta aaggcgatta ctgaacagcg tcgccccgcc agcgaggcag
    30121 ccttgctgta tgaagagact gcggaaagtg tagagaaacg cgaaaaaatg gcgatggcac
    30181 gtaaacttaa tgccttaacc atgccgcacc cggaccgacg cccggacaaa aaagagcgcc
    30241 gcgacctgtt acgatttaaa cacggcgaca gtgaataact gtcacctgca agagagatga
    30301 ttatgccgca acatgaccaa ttacatcgct atctgtttga aaattttgcc gttcgcggcg
    30361 aactggtaac cgtttcggaa accctgcaac agatccttga gaaccacgat tatccgcagc
    30421 ccgttaaaaa cgtgctggca gaactgctgg ttgcgaccag cctgttaacc gctacgctga
    30481 agtttgatgg tgatatcacc gtacagctgc aaggcgacgg tccgatgaat ctggcggtta
    30541 ttaacggtaa caataaccag caaatgcgcg gtgtggcgcg cgtgcagggc gaaattccag
    30601 aaaatgccga cctgaaaacg ctggtcggca atggttacgt ggtgatcacc attaccccga
    30661 gcgaaggcga acgctatcag ggcgtggttg gtctggaagg tgataccctg gcggcctgcc
    30721 tggaagatta ctttatgcgt tctgaacagc tgccgacgcg cctgtttatt cgcaccggcg
    30781 acgtagacgg caaaccggct gcaggcggta tgttgttgca ggtaatgcct gcgcaaaatg
    30841 cccagcagga cgactttgac cacctggcga cgctaaccga aaccatcaaa accgaagaac
    30901 tgctgacctt accggcaaac gaagtgttgt ggcgtttgta tcacgaagaa gaggtgacgg
    30961 tttacgatcc gcaggatgtg gagttcaaat gcacctgctc gcgtgaacgt tgcgccgatg
    31021 cgctgaaaac gctgcctgat gaagaagttg atagcatcct gacggaagat ggcgaaattg
    31081 acatgcattg tgattactgc ggtaaccact atctgttcaa tgcgatggat attgctgaaa
    31141 tccgcaacaa cgcgtctccg gcagatccgc aagttcatta atgagtatgt ccggcagaga
    31201 gggctctgcc ggattttacg acgtaatggc ctgatgtgct gcgcttacca ggcctgtgaa
    31261 ataaatcgca aattattgtt ggtcagatac agcgtcgtat ccgttaaaag agttgtcact
    31321 tacttaagtt tatttccagc gtttcaccat ctaccgtcag atcgcgccac gctttcggcc
    31381 tcgtccccag ttttccatcc ttcgcagctg tgagtaattt ttctttcgtt atttcgttcg
    31441 gaagtaaact catatcaagc gcgtcccatt cttttctgtc tgagtcaaag ccatagacaa
    31501 tgactctgtc atcattaaaa gcaaacagga tccgctccgc ctgaccatca ctattcagat
    31561 cttgctcgac caggacgcag gcgtcttttt caatacaggt catcacgtta tagcgatcct
    31621 ggattaaggc cgaccagaat gtcgcatccg gtttaacaga accaggggca attaacacat
    31681 tttcggctaa tactttttcc gatacctgtt cctgaagatg ttgctctcca tctaacgcca
    31741 tcagcagatc ccgcgcgcgt ttcgggtctt tcataaaccc ggcatcgctt ttcagcgact
    31801 caagcgccgc acgtccatag cgaccgctct gttcgagcat gtagatagtt acctggtctg
    31861 gcgtgttttt gccgctctga taacgcgcca tatgactgtt cacgctaata cgcatactat
    31921 ccagcaccgg cgaattaaga agcaccagta tcaccaacac caataacgaa accgcaaggt
    31981 tcactttgcc ctgaagaaca aggggatttt gcccattacg ccagacgata ctgacaaaat
    32041 accccagcga ccacaccagt aacaccagca ccgccagcgc gccctgcaag cggtcgacag
    32101 tccagccgta ctgagcgacc cgcagccata acgcccaggc ggcgacgaac acatacagcg
    32161 gagcaaccag caaagcggtt ttaatcaggc aacgcaacgg ccccgtccag ggaagtgacg
    32221 ctttttgcgg atcgcggaca atagccatta agatcaattg caaaaaggcc agcgttaaca
    32281 gcaacccggc ggcggagatg tggcgagaaa tcgcgctcag gcccgtaaac ggcagagtga
    32341 tgataaacat cagggttagc aatgatacta acggcagcaa ccccgtggcg attaacgtga
    32401 acaacttttg aatagagtcg attaaacgtg actgtgtgcg cgcgagaatc accgccagtg
    32461 cggtaaccag acctaatgta agatacataa accagtcggt tgcaaaaaag agcgtattga
    32521 aaaacttgat gccaacaagt ttaaacaact caccccacag taaaagcacc acccacgtca
    32581 ggccattggc gagaaaaatc accagtaata taagtacgtt atgccatact gacttgtaaa
    32641 agtagctata gcggctactg ccattgcgga tacgaaggct ttgttgtatc cacggtagca
    32701 acaacattgc catcaacagc agatagcagc caaaatccca aatcgccttt tcagctctcc
    32761 aggggttcat gccatcagtt tgccacttca gccaaccgct catccctgtc gtggcaataa
    32821 acaccagcgc cagccatccc caaaggcgct tctgtttaaa agagatcacg gagaagagta
    32881 aaacggatga aaacgcaacg gtcgcaggca cgccataaac aatccaactg tgattttttc
    32941 cggcaagcca ggcgatcaac aggtagcaaa ccagcccctg aagtaatccg gtagcaatca
    33001 tcccccagcg tgttgccggt gaaagttcaa cgttatccat gttggttatc cagaatcaaa
    33061 aggtgggtta attatcgcat ccgggcagta gtattttgct tttttcagaa aataatcaaa
    33121 aaaagttagc gtggtgaatc gatactttac cgattgaatt tgcatcaatt tcattaagga
    33181 atgcgattcc actcacaata ttcccgccat ataaaccaag atttaacctt ttgagaacat
    33241 tttccacacc taaaatgcta tttctgcgat aatagcaacc gtttcgtgac aggaatcacg
    33301 gagttttttg tcaaatatga atttctccag atacgtaaat ctatgagcct tgtcgcggtt
    33361 aacaccccca aaaagacttt actattcagg caatacatat tggctaagga gcagtgaaat
    33421 gcgcgttaac aatggtttga ccccgcaaga actcgaggct tatggtatca gtgacgtaca
    33481 tgatatcgtt tacaacccaa gctacgacct gctgtatcag gaagagctcg atccgagcct
    33541 gacaggttat gagcgcgggg tgttaactaa tctgggtgcc gttgccgtcg ataccgggat
    33601 cttcaccggt cgttcaccaa aagataagta tatcgtccgt gacgatacca ctcgcgatac
    33661 tttctggtgg gcagacaaag gcaaaggtaa gaacgacaac aaacccctct ctccggaaac
    33721 ctggcagcat ctgaaaggcc tggtgaccag gcagctttcc ggcaaacgtc tgttcgttgt
    33781 cgacgctttc tgtggtgcga atccggatac tcgtctttcc gtccgtttca tcaccgaagt
    33841 ggcctggcag gcgcattttg tcaaaaacat gtttattcgc ccgagcgatg aagagctggc
    33901 aggtttcaaa ccagacttta tcgttatgaa cggcgcgaag tgcactaacc cgcagtggaa
    33961 agaactgggt ctgaactccg aaaacttcgt ggcgtttaac ctgaccgagc gcatgcagct
    34021 gattggcggc acctggtacg gcggcgaaat gaagaaaggg atgttctcga tgatgaacta
    34081 cctgctgccg ctgaaaggta tcgcttctat gcactgctcc gccaacgttg gtgagaaagg
    34141 cgatgttgcg gtgttcttcg gcctttccgg caccggtaaa accacccttt ccaccgaccc
    34201 gaaacgtcgc ctgattggtg atgacgagca cggctgggac gatgacggcg tgtttaactt
    34261 cgaaggcggc tgctacgcaa aaactatcaa gctgtcgaaa gaagcggaac ctgaaatcta
    34321 caacgctatc cgtcgcgatg cgctgctgga aaacgtcacc gtgcgtgaag atggcactat
    34381 cgactttgat gatggttcaa aaaccgagaa cacccgcgtt tcttatccga tctatcacat
    34441 cgataacatt gttaagccgg tttccaaagc gggccacgcg actaaggtta tcttcctgac
    34501 tgctgatgct ttcggcgtgt tgccgccggt ttctcgcctg actgccgatc aaacccagta
    34561 tcacttcctc tctggcttca ccgccaaact ggccggtact gagcgtggca tcaccgaacc
    34621 gacgccaacc ttctccgctt gcttcggcgc ggcgttcctg tcgctgcacc cgactcagta
    34681 cgcagaagtg ctggtgaaac gtatgcaggc ggcgggcgcg caggcttatc tggttaacac
    34741 tggctggaac ggcactggca aacgtatctc gattaaagat acccgcgcca ttatcgacgc
    34801 catcctcaac ggttcgctgg ataatgcaga aaccttcact ctgccgatgt ttaacctggc
    34861 gatcccaacc gaactgccgg gcgtagacac gaagattctc gatccgcgta acacctacgc
    34921 ttctccggaa cagtggcagg aaaaagccga aaccctggcg aaactgttta tcgacaactt
    34981 cgataaatac accgacaccc ctgcgggtgc cgcgctggta gcggctggtc cgaaactgta
    35041 atgatttgaa gctggagaat atctatccag tatcttatag aaagcaaaac gggaggcacc
    35101 ttcgcctccc gtttatttac ccttcttttg tcgtgccctg cgcccgcgtt accggcactg
    35161 gcagccaggc gcgaatggaa agcccgcccc gctcgctggt gccaagctcc agcatcccgt
    35221 tatggttatc cacgatacgc tgcacaattg ccagccctaa tcccgtgccg ctaatggtgc
    35281 gcgcactgtc gccgcggaca aacggctgga acaggtgctt acgttgttcc ggcgcaattc
    35341 ccggaccgtc atcttccacc tggaaccagg cgcgattcgg ctccgttccg ctgctgactt
    35401 tgatccagcc attgccataa cgggcggcgt tgaccaccat attcgccacc gcgcgtttga
    35461 tcgacagcgg gtgcattttc acgtcaatgc tgccggggta aagcgcggtt tcaatttccc
    35521 gctcatagcc actttcggca gcaatcacct caccgagtac tgcattaaga tccgccattt
    35581 ccatcggcat ctcctgcccg gtgcgcaggt agtcgataaa ctgctcaatg atggcgttgc
    35641 actcttcgat atctttattg atcgattctg ccagatagcc atcctgctcg ctcatcatct
    35701 cagtcgccag gcgaatacgc gtcagcggcg tgcgcaagtc gtgacttacc cccgccatca
    35761 gcagcgtgcg gtcatccgcc agttgcttaa caccagccgc catatggtta aaggcacggg
    35821 taacggaacg cacctcggac gcgccatact cacgcagcgg cggcggaata atccctttac
    35881 caacctgcaa ggctgcgtgt tcgagatcga ccaacggtcg gttctggata cgaataaaca
    35941 gccacgcccc gcctatcgcc aatagcataa tcgccagcgt atagcggaac agcggagaga
    36001 aatcgccctg atgaatttcg gtcagcggca cgcgtaccca gatattgggc gacagccagg
    36061 ttttcagcca gacgacaggc gaacttttgt tgacctcaac gcgcacttcc gtcgggccgc
    36121 ccagttgctg tgccatctga tggcttaaga attcatagtg ttgcgcccaa cgcagacctg
    36181 cctcttcggc agcctcgttg gagtagagag agatccccag ctcacggtag atctcccgac
    36241 ggaaagcggg aggcacaacc aactgcgtgc cgtcctccag ttgcagtttg tcggtcatca
    36301 acatacgcac ttcgtacgcg aggactttat taaactgctg gaggctcggc aaaatcgcga
    36361 agttcagcac caccagataa gtcgtcacca ggctggcgaa cagcaaggtg acgatgagca
    36421 ataacgtacg ggcaaatgaa cttcgtggcg agaagcgcaa tcgcctcatg ctttagagcc
    36481 gtccggtaca aagacgtagc ccaggcccca aacggtctga atgtaacgcg gatgcgctgg
    36541 atcttcttct accatgcggc gcagacgcga aatctgcacg tcgatggagc gttccattgc
    36601 ggaatattca cgaccacggg caaggttcat cagcttatcg cgggagagcg gctcacgcgg
    36661 atggctgacc agtgccttca gtaccgcaaa ctcaccgctg gtgagcggca tcggctcatc
    36721 ttcgcggaac atttcgcgcg taccgaggtt aagtttgaac ttaccgaaag caattaccgc
    36781 ctcttcctgt gacggtgcgc ctggcagttc gttcgcctga cgacgcagca ccgcacgaat
    36841 acgggccagc agttcacgcg ggttaaacgg ttttggaatg tagtcgtcag cgccaatctc
    36901 caggcctacg atacggtcca cttcttcccc tttcgccgtc accataatga tcggcatcgg
    36961 gttgctctga ctacgaagac gtcggcaaat cgacaagcca tcttcaccag gtaacattaa
    37021 atccagtacc ataagatgga aagattcacg agtcagcagg cgatccatct gttctgcatt
    37081 agcgacgctt cgaacctgga agccttgttc ggtgagataa cgttccagca gcgcacgcag
    37141 gcgcatgtcg tcatcgacca ccagaatctt gtagttctct tgcattgttt gtactcccaa
    37201 aggttcgcaa caatttgtaa gcgtgtattc ttaaaaaagc tcacgttcgt caccagctaa
    37261 atctggtatg aatttcagcc taaattgtta caaagcatat taaacagcag cttaagtata
    37321 caatttattc ggcgaaacat tattgattct gttgatatga tcacgttata cccaatgtgc
    37381 gcattatcaa acagacaaag ggaatcaacg agatgaaaac gcccctggtt acccgggaag
    37441 ggtatgaaaa actcaaacaa gagcttaatt atctctggcg tgaagaacgc ccggaggtca
    37501 caaaaaaggt gacctgggcc gcaagtctgg gcgaccgcag cgaaaatgct gactatcagt
    37561 ataataaaaa gcgtctgcgt gaaatcgacc gtcgcgtgcg ctatctcact aaatgcatgg
    37621 aaaatctcaa aatcgtcgat tactcccctc agcaggaagg caaggtcttt tttggcgcat
    37681 gggtggagat tgaaaacgac gatggcgtga ctcaccgttt ccgtattgtc ggctacgatg
    37741 aaatttttgg ccgtaaagat tacatctcta tcgattcccc gatggcccgc gcattgctga
    37801 aaaaagaagt cggcgatctg gcggtggtga atacccctgc cggggaagcg agctggtatg
    37861 tcaatgctat cgagtacgtg aaaccgtaag gaagagtctt aacctcctgc cgatggctgg
    37921 catttttgcc agccagtccg tataactatc ccctgatttt tgatccgaaa agatgaactc
    37981 aaaccatgat gaatgattcg ttctgccgca ttattgcggg tgaaattcag gcgcgcccgg
    38041 aacaggttga cgctgccgtt cgcctgcttg acgaagggaa taccgtgccg tttatcgcac
    38101 gttatcgtaa ggaaatcacc ggcggtctgg atgacacgca gctgcgtaat ctggaaacgc
    38161 gtctgagcta tctgcgcgag ctggaagaga gacgtcaggc gatcctcaaa tccatttccg
    38221 agcaaggcaa actcaccgat gatctggcga aggccatcaa cgccacacta agcaaaaccg
    38281 aactcgaaga tctctacctg ccctacaaac ctaaacgccg cacccgcggg caaatcgcca
    38341 ttgaagcagg gcttgagccg ttggctgacc tgctgtggag cgatccgtca cacacgccag
    38401 aagtcgccgc tgcacaatat gttgatgcag ataaaggcgt ggcagatacc aaagccgcgc
    38461 tggacggcgc gcgctatatc ctgatggaac ggtttgccga agatgccgcg ctgctggcga
    38521 aagtgcgtga ttatctgtgg aagaacgcgc atctggtctc aaccgtgatg agcggtaaag
    38581 aagaggaagg ggcaaaattc cgcgactatt tcgatcatca cgaaccgctg tccacggtgc
    38641 cttctcaccg cgcgctggcg atgttccgtg ggcgtaacga aggcgtactc cagctttcgc
    38701 tgaatgccga tccgcagttc gaagagccgc caaaagagag ctattgcgag caaatcatca
    38761 tggatcatct cggcctgcgc ctgaacaatg ctccggcgga tagctggcgc aaaggcgtgg
    38821 tgagctggac ctggcgcatc aaggtgctga tgcatctgga aaccgaactg atgggcaccg
    38881 tgcgcgaacg cgcggaagat gaagcaatca acgtctttgc ccgtaacctg cacgatctgc
    38941 tgatggcggc ccctgccgga ctgcgtgcca cgatgggcct cgatccgggt ctgcgtaccg
    39001 gggtaaaagt agcggtggtc gatgccactg gcaaactggt ggcgaccgat accatttacc
    39061 cgcacaccgg acaaacagca aaagcagcga tgaccgttgc tgccttgtgt gaaaagcata
    39121 acgttgaact ggtggcgatc ggcaacggta cagcttcccg cgaaaccgaa cgtttctatc
    39181 tcgacgtgca gaagcagttc ccgaaagtga ccgcgcagaa agtgatcgtc agcgaagctg
    39241 gcgcgtcggt ttactcggct tccgagctgg cagcgcagga gttcccggat ctcgacgttt
    39301 cgctgcgtgg tgcggtatct atcgcccgcc gtttgcagga tccgctggcg gagctggtga
    39361 aaatcgatcc gaaatctatc ggcgtaggtc agtatcagca tgacgtcagc cagacgcaac
    39421 tggcccgcaa actggatgca gtagtagaag actgcgtaaa cgccgttggc gtcgatctca
    39481 acaccgcttc tgttccgcta ttaacccgcg tggcgggcct gacgcgcatg atggcgcaaa
    39541 acatcgtggc ctggcgcgat gagaacggcc agttcaagaa ccgtcagcaa ctgctgaaag
    39601 tcagccgtct ggggccgaaa gccttcgagc agtgcgcggg cttcctgcgc attaaccacg
    39661 gtgataaccc gctggacgcc tctaccgttc acccggaagc ctatccggtg gtggaacgca
    39721 ttctggcagc aacacagcag gcactgaaag atctgatggg taacagcagc gaactgcgta
    39781 acctgaaagc gtctgacttt actgatgaaa aattcggtgt gccgacagta actgacatca
    39841 tcaaagagct ggaaaaaccg ggtcgcgatc cgcgtccgga atttaaaacc gctcagtttg
    39901 ccgatggcgt cgagacaatg aacgacctgc aaccgggtat gatcctcgaa ggcgctgtca
    39961 ccaacgtcac caactttggc gcgtttgtcg atattggcgt gcatcaggac ggcctggttc
    40021 acatctcttc attgtcgaac aagtttgtgg aagatccgca taccgtggtg aaagcgggcg
    40081 acattgtgaa ggtgaaagtg ctggaagtgg atcttcagcg taaacgtatc gccctgacca
    40141 tgcgtctgga cgagcagcct ggcgaaacca acgcccgtcg cggcggcggt aatgaacgcc
    40201 cgcaaaacaa ccgcccggca gccaaaccac gcggtcgtga agcgcagcct gccggtaata
    40261 gcgcgatgat ggatgcgctg gcggcggcaa tgggcaaaaa acgttaaacg ccctgattga
    40321 cccctaaacc tgcgcgataa ggtgtccacg ccgcatctgg caacgaacgc cttgtctgac
    40381 ataaaagtgc cggagaatat ctccggcatt tttattccac agccaaactc acaatatatt
    40441 ccggcaacat ttatcacttc attaacagct gaaaccttaa ttaaacatta gccagtcctg
    40501 gtaattcgct attcgaatta tattttcgct gcgatataac cttgagccac atcaacattg
    40561 agtcagatta ttattcaaac caacattcgc acacatttta agtattgctg atagaaacca
    40621 ttctcattat cattgtgctg ttgattattt aatctctcct tcgttggcaa atcatctggt
    40681 ctcatgtcgc tgtcaaacgc cccatgaggt agttatccag ttaatgagaa acaagtaggc
    40741 acctatgcaa tacactccag atactgcgtg gaaaatcact ggcttttccc gtgaaatcag
    40801 cccggcatat cgccaaaaac tgctttctct tggcatgtta cctggctcct cttttaatgt
    40861 ggtgcgcgtc gctccactcg gcgaccccat tcatatcgaa acccgtcgtg tgagcctggt
    40921 attacgcaaa aaagatctgg ccttattaga agtggaagcg gtttcctgtt aatacggtga
    40981 taacaacaat gaaaaaatta accataggct taattggtaa tccaaattct ggcaagacaa
    41041 ccttatttaa ccagctcact ggcgcacgtc agcgtgtagg taactgggct ggcgttaccg
    41101 tcgaacgtaa agaagggcaa ttctccacca ccgatcatca ggtcacgctg gtggacctgc
    41161 ccggcaccta ttctctgacc accatctcat cgcagacctc gctcgatgag caaatcgcct
    41221 gtcactacat tttgagtggc gacgccgacc tgctgattaa cgtggtggat gcgtctaacc
    41281 ttgagcgtaa cctgtacctg acgctacaac tgctggaact cggcattccc tgcatcgtgg
    41341 cactgaacat gctcgacatt gccgagaagc aaaatattcg tattgaaatt gatgctctgt
    41401 cggcgcgtct tggctgccca gtgatcccgc tggtttcaac ccgtggtcgc ggtattgaag
    41461 cgctcaagct ggcgattgat cgctataaag ctaacgagaa tgtggaactg gtgcattacg
    41521 cacagccgct actcaacgaa gcagattcac tggcaaaagt gatgccttcc gacatcccgc
    41581 tgaaacaacg tcgctggctg ggcctgcaaa tgctggaagg cgatatctac agccgcgcct
    41641 acgccggtga agcgtcgcag catctggatg ccgccctcgc ccgtctgcgt aatgagatgg
    41701 acgatccggc gctgcacatt gccgatgcgc gttaccagtg cattgctgcc atctgtgatg
    41761 tggtaagcaa caccctgacg gcagaaccca gccgtttcac cactgcggta gataaaatcg
    41821 tgctcaaccg tttcctcggt ctgccgattt tcctctttgt gatgtacctg atgttcctgc
    41881 tggctatcaa catcggcggg gcgttacagc cgctgtttga cgtcggctcc gtggcgctat
    41941 ttgtgcatgg tattcaatgg attggctaca cgctccactt cccggactgg ctgactatct
    42001 tcctcgccca gggcctgggt ggcggcatta acaccgtgct gccactggtg ccgcagattg
    42061 gcatgatgta cctgttcctc tccttccttg aggactccgg gtatatggcg cgtgcggcgt
    42121 ttgtgatgga ccgtctgatg caggcgctgg gcttgccggg gaaatccttt gtgccgctga
    42181 tcgtcggttt cggttgtaac gtaccgtcgg taatgggtgc acgtacgctt gatgcaccgc
    42241 gtgaacgtct gatgaccatc atgatggcac cgtttatgtc ctgcggcgcg cgtctggcta
    42301 tcttcgcagt attcgcggct gccttcttcg ggcagaacgg tgcgctggcg gtcttctcgc
    42361 tgtatatgct gggtattgtg atggcggtgc tgactggcct gatgctcaag tacaccatca
    42421 tgcgcggtga agcgacgccg tttgtcatgg agttgccggt ctatcatgta ccacacgtta
    42481 aaagcctgat tatccagacc tggcagcgtc tgaaaggctt cgttctgcgt gctggtaaag
    42541 tgatcatcat cgtcagcatt ttcctgagcg ctttcaacag cttctcgctg agcgggaaaa
    42601 tcgtcgataa catcaacgac tcggcgctgg cgtccgtcag ccgggtgatc accccggtct
    42661 tcaagccaat tggcgtgcat gaagataact ggcaggcaac ggttggcctg tttacaggtg
    42721 ccatggcgaa agaagtggta gtgggtacgc tcaacaccct ctacaccgca gaaaatattc
    42781 aggacgaaga gttcaatccg gcagagttta acctcggtga agagctgttc agtgcgatag
    42841 atgaaacctg gcagagcctg aaagacacct tcagccttag cgtactgatg aaccccattg
    42901 aagccagcaa aggcgacggc gaaatgggta ccggggcgat gggcgtgatg gatcagaaat
    42961 tcggtagcgc agcagcagct tacagctacc tgattttcgt cctgctgtat gtaccatgta
    43021 tctcggtgat gggagccatc gcccgtgaat caagccgtgg ctggatgggc ttctccatcc
    43081 tgtgggggct gaatatcgct tactcgctgg caacattgtt ctatcaggtc gccagctaca
    43141 gtcagcaccc aacttacagc ctggtgtgca ttctggcggt tatcctgttt aacatcgtgg
    43201 ttatcggtct gctgcgccgc gcgcgtagcc gggtggatat cgaactgctg gcaacccgca
    43261 agtcggtaag cagttgctgc gcagccagca ccaccggtga ttgccattaa tggcttcact
    43321 tattcaggtg cgcgatttgc tggcgttacg gggccgtatg gaagcggccc agataagcca
    43381 gacattgaac actccacagc caatgattaa cgccatgctg caacaactgg aaagtatggg
    43441 caaagccgtg cggattcagg aagaacctga cggctgcctc tctggcagtt gtaaaagctg
    43501 cccggaagga aaagcctgtc tgcgcgagtg gtgggcgctg cgttaacctt actccatcgc
    43561 ctgttttgga aagcccggta tgcgtctgca tccgggcttt tttgcgtgcg gctttccata
    43621 aaaatgcaac tcttgcagca cggcgtaagt tcctttgaaa gcatctcgca gggatgaaaa
    43681 ctcgctaata cacaggtgtg gagtggcgcg tagagtcgcg gcattcaaac aacaggtgaa
    43741 ggaacgccat gagcaaaaag cagagttcca ccccacacga tgcgctgttc aaactctttt
    43801 tacgccaacc ggacacggct cgtgattttc ttgcgtttca tttaccggca cccattcacg
    43861 cgctttgtga tatgaaaacc ctcaagctgg agtcgagcag ctttattgat gacgatctgc
    43921 gtgaaagcta ttccgatgtg ctgtggtcgg tgaaaacgga acaaggacca ggatacatct
    43981 attgtctgat tgaacatcaa agcacctcaa acaaactgat cgcatttcgc atgatgcgtt
    44041 acgctattgc cgcaatgcaa aatcaccttg atgctggata caaaacgttg ccgatggtgg
    44101 tgccattgtt gttttaccac ggtattgaaa gcccctatcc ctattcgctg tgttggctgg
    44161 attgtttcgc cgatcccaaa ctggcaaggc agctttatgc ctccgcattt ccgctgattg
    44221 atgtcaccgt catgcctgat gatgaaatca tgcagcaccg acgtatggcg ctgctggagt
    44281 taattcaaaa acatattcgt caacgcgatc tgatggggct ggtagagcaa atggcctgct
    44341 tattaagtag tggatacgct aatgacagac aaatcaaagg gctgtttaat tacatactgc
    44401 aaaccggcga cgcggtacgt tttaacgatt ttatcgacgg cgttgccgaa cgttcaccga
    44461 aacacaagga gagtttaatg acaattgcgg aaagattgcg gcaggagggg gaacaatcca
    44521 aagccctgca tatagccaaa ataatgcttg aatccggagt ccctcttaca gacatcatgc
    44581 gctttaccgg ggtgtcagaa gaagagttgg ctgcggcgag ccagtaaatt tctgtctcgc
    44641 catttcaaaa gttgcctaca ccctctgctt caacgccacc agcaggtgac aaaactcgac
    44701 cggatgcgaa ataaacggcg catgggccgc tttggcgaag atatatgatt cgctgtgagg
    44761 ccaaagttta tccagcatcg gcaccacttt gcgcggcacc agaccgtcga gatagccata
    44821 caatcgcaaa aacggcatgg acacgttttg cagcggctga cggagatcga ccgttttcag
    44881 gatttccagc ccgccattaa gcacgtcaac ctccggcatc ggtaacgcca gaacggtttt
    44941 cttcaacgcc cgcgcatcct ggcgcgccgt ttcagtcccc atggtttgta acgccaggaa
    45001 ccgctccact gtacgctgaa aatcatcact gagttgctgc tgaaatcccg ccagtacgtc
    45061 cggttttatc cccggccact cgtcacgagc actaaaacaa ggtgacgacg ccacggtgac
    45121 cagcgcctga acacgctcgg gatgggttaa aacaatttgg cttgccacca gcccgcccag
    45181 actccagcct aaccaaatgg ctttatcagg tgcctgttgc agcacggctt cggccatatc
    45241 agcaagtgac agcgcaccaa atccccggct acgcccgaag ccgggcaggt caacaaggtg
    45301 cagcgtaaaa tgcgagctaa gttcctcgtc aatgcaacgc cacacttcgg cattcagtcc
    45361 ccatccgtgc agcagcacaa gatgaacatt cccctgacct ttggtctgcc accagatgtt
    45421 attcatccgc tattgttctc ttttgactta caaggatgaa catatgctaa cagtaccggg
    45481 attatgctgg ctatgccgaa tgccactggc gttaggtcat tgggggattt gttcggtctg
    45541 ttcacgcgcc acccgcacag ataaaacgtt atgcccacaa tgtggattac ccgccacaca
    45601 ctcccatctt ccctgcggtc gctgcctgca aaaaccgccg ccctggcaaa gactggtcac
    45661 ggttgccgac tatgcgccgc cgttaagtcc gcttatccac cagcttaaat tttcccggcg
    45721 cagtgaaatc gccagcgccc tgtcacgtct gttactattg gaagtcttac acgctcgtcg
    45781 caccaccggg ttgcaattgc cggatcgcat catcagcgtt ccgttatggc agcggcgtca
    45841 ctggcgtcgg ggatttaatc agagcgattt gctgtgtcag ccgttatcac gctggttgca
    45901 ttgccaatgg gatagcgaag ccgtcacacg tacacgggcc actgcgaccc agcattttct
    45961 cagtgcccgg ctgcgcaagc gcaacctgaa aaatgccttt cgtcttgaat tgcccgtgca
    46021 aggtcgccat atggtgattg tggatgatgt cgttaccacc ggaagtaccg tcgcagagat
    46081 tgcgcagttg cttttacgca atggtgcggc gactgtccag gtctggtgcc tttgtcgaac
    46141 cttgtagagc ctcgatgatg ggcgtattat aaccaactaa aatagtcaac tattaggcca
    46201 ttactatgat ccgtatttcc gatgctgcac aagcgcactt tgccaaactg ctggcaaatc
    46261 aggaagaagg gacacaaatc cgcgtatttg tgattaaccc tggcacgcct aacgctgaat
    46321 gtggtgtttc ttattgtccg ccggatgccg tggaagccac cgacactgcc ctgaaatttg
    46381 acctgctgac cgcgtatgtt gatgagttaa gcgcaccata cctggaagat gcagagatcg
    46441 actttgttac cgaccagttg ggttctcagt taacgctgaa agccccgaac gccaaaatgc
    46501 gtaaagtggc agacgatgca ccgctgatgg agcgcgtgga gtatatgctg cagtcgcaga
    46561 tcaacccaca gcttgctggt cacggtggtc gcgtttcgct gatggaaatc accgaagacg
    46621 gttacgccat tctgcaattt ggcggcggct gtaacggttg ttccatggtc gatgtgacgc
    46681 tgaaagaagg gatcgagaag cagctgctga acgaattccc ggagctgaaa ggtgtgcgcg
    46741 atctcaccga acaccagcgc ggcgaacact cctactacta agttatcccc tcttttgtag
    46801 attgcccgat gcgacgctaa agcgtcattt cgggcctacg ttgaacatgt gccagacgtt
    46861 ggcacagcat tgctttggca aatccctccc ttcgtttccc cgcgataata tgaccaacct
    46921 ctcataattt aaatttaccc cgctctggtg attctcaaac gccagatgtt acccgtatca
    46981 ttcacatggg taccaaacat actcctgaca tctgactaca ataattagtt ttagtgggta
    47041 tcagtcgtgg tgccgcaata tctctgttcc cgattgggat aattagagtt tgtcgtcaga
    47101 aaattgacgt tacccataac aaatgaaagg ccaggtaaat catgccatta gtcattgttg
    47161 ctatcggtgt aatcttgttg ttgctcctga tgatccgctt caaaatgaac ggcttcatcg
    47221 ctctcgtcct cgtggcgctt gctgttggat taatgcaagg aatgccgctg gataaagtta
    47281 ttggctccat caaagccggt gtcggcggga cgctcggtag ccttgccctg atcatgggtt
    47341 ttggcgcaat gctgggcaaa atgctggcag actgcggtgg cgcacaacgt atcgccacca
    47401 cgctgattgc caaatttggt aaaaaacaca tccagtgggc ggtggtactg accggtttta
    47461 ccgttggttt tgccctgttc tatgaagtgg gctttgtgct gatgctgccg ctggtgttta
    47521 ccatcgcggc ttctgcgaat attccactgt tgtatgttgg tgtaccaatg gcagctgcac
    47581 tgtctgtgac ccacggcttc ctgccaccgc atccgggtcc gactgcgatt gccaccattt
    47641 tcaatgccga tatgggtaaa accctgctgt acggtactat tctggcaatc ccgaccgtga
    47701 ttctcgccgg tccggtttac gctcgcgtgt tgaaaggtat cgataagcca attccggaag
    47761 gtctctacag cgcgaaaacc ttcagcgaag aagagatgcc gagctttggc gtcagcgtct
    47821 ggacctctct ggtgccggta gtactgatgg cgatgcgtgc gattgccgaa atgatcctgc
    47881 cgaaaggtca cgctttcctg ccggtagcgg agttcctcgg tgacccggta atggctacgc
    47941 tgattgccgt gctgattgcc atgttcacct ttggtctgaa ccgtggtcgt tcaatggatc
    48001 agattaacga cacgctggtt tcttccatca aaatcattgc gatgatgctg ttgatcatcg
    48061 gtggtggcgg tgcgttcaag caggtgctgg tagacagcgg cgtggacaaa tacattgctt
    48121 ccatgatgca cgaaaccaac atttctccgc tgctgatggc ctggtcgatt gctgccgtac
    48181 tgcgtatcgc gctgggttcc gcaaccgttg cggcaatcac tgcgggtggt atcgcggcac
    48241 cgctgattgc aacgacgggt gttagcccgg agctgatggt tattgcggtg ggttccggta
    48301 gtgtgatttt ctctcatgtg aacgatccgg gcttctggct gttcaaagag tactttaacc
    48361 tgactatcgg cgagaccatc aaatcctggt cgatgctgga aaccattatc tcggtgtgcg
    48421 gtctggtagg ctgtctgctg ctgaatatgg tgatttgata acactgactg ccggatgcat
    48481 cgcatccggc atcaaccgca ctctacttct tcttcgctgc agctctgcgc cgtctgtcca
    48541 aatccttcag caacttgttc acgccatcat cggcaaacat cgactcaagc gttgcggaaa
    48601 gcttgcgtcg ccagttttta tactggtaac tggtgccagg aatattcacc ggttcggcca
    48661 tatccagcca gtcttccggc tgtagtccta acagagcact gttactgtcg gcaatgtagc
    48721 gctgcaaacc acggttcagc gtcggcgtca tcgacatcaa cgatgcctta tgcccggcac
    48781 gttttggcag acaaccatat ttatgcagtg catccagcag cccttgcttc gccagttcgc
    48841 gatcctgata cagaccgcgc agtaccactt catccggata cagccccagg gttttgccca
    48901 gcgttagatc cccgctctcc cagtaaccgc gtagcgtagg caggtcatgt gtcgccgcaa
    48961 ccgccatcga ctgctccgga tacgctttcg gtgcacggaa cgtcttctcg tggtcgtttt
    49021 cgaaatagag cactttgtag gagtacacac cgctgctgcg cagcttaccg acaatctcta
    49081 ccggtacggt accgagatct tcaccaatca ccatacagcg atgacgttta ctttcgagtg
    49141 ccagaatcga gagcagatca tccaccggat agtgaacata cgcgccctga tctgccgtct
    49201 cgccatacgg tatccaccac aaacgcagca tcgacatcac atggtcaatt cgtaatgcgc
    49261 cgcagttttg catattggca cgcaacagct cgataaacgg ttcataggca cgcgcggtga
    49321 tgatatgcgg gtccattggc ggtaatcccc agttctgccc caacgggccg aggatatccg
    49381 gcggcgcgcc aaccgacgct ttcaggcaat atagttcacg gtcacaccag gtttccgccc
    49441 caccttccgc tacgccaacc gccagatcac gatacaagcc aatcggcatt tcatagccct
    49501 ggcttatctc ccagcaggcg gcaaactggc tgtaagccag ccactgcaac cagagataaa
    49561 aatcgacgtc atcacgatgt tcttcgcaga actgacgcac ttctggtgaa tccacgttct
    49621 gatacatctc tggccatgca ggccagcccc agcgcatttc gtcctctttc acttgctggg
    49681 catgtagcgc atcaaaggct gcctgccaga acaggctgtc gccctgctct gcaacaaact
    49741 ggcgaaacgc ggtcatctgc tcatcatcac gttgcgcgaa acctttccac gccattcgta
    49801 atgctgtcat ttttagggcg gtaaccgtgg agtaatcgac ccagtcggca tcgcgcgcct
    49861 gttgcagcgt ctgttgcgtg gtcggcaact gccaccaggc ctgagcctct tcgctaagat
    49921 ggaaatcttc aacggcgtta acgtcgatat aaatcacatt cagccaacgg cgagaagacg
    49981 ggctgtatgg gctggcactc tccggatttg ccggatagag cgcatgaatc gggttcaggc
    50041 cgatgaacga cccgccacgt tttgccacat ccaccagcat cgctttgaga tcgccaaaat
    50101 ccccaatacc ccagtttttt tccgatcgca gcgtataaag ctgaacgcag gcaccccaca
    50161 gcttttgttt attcagcaac gcctgcggtt cgtaacagcg tttcggggcg acaatcaccc
    50221 ggcaatgcgc gcgctggtcg tcctgggtga gtgtcagcgt gtgataacct tccggcagct
    50281 tcgtcggtag attgaacgct ttgccccccg ttacatggcc tttgtactgt gttccttctt
    50341 cggtggtcag cagccagcta tattcgccgc tgccctccac caccatcggc atttttttgc
    50401 cgctggtata aaccatgaca ttcgggactg gcgttaccgc cactttcgtg gcggtacgtt
    50461 gatgcatcgc gtcaagcaaa cgccgtttgg tttcggcgct aatcgactgc ggtttaccgt
    50521 gggcattgat gtaattgggg ctaatccccg ccgccagcgc ggcattatcc agacgtttgc
    50581 tttccatcga gcttccttag cgttttgcct gccagatacg agcctgataa tcgcgaatag
    50641 agcgatccga gctaaacata ccgcagcggg cggtattgag gatcgccgcg cgagtccagg
    50701 cctcctggtc gcggtacagc acatccacct gcttttgtgc ctcaacatag gctgcgaaat
    50761 ccgccatcac cagatacgga tcgccgcctt gtttgccgat actgtgcagc atctggtcga
    50821 aggcatgctt atcgccgtcg ctgtatttac cgctttccag ctctttcaat actgcgtcca
    50881 gcaccttatc tttcttccgc catttcaccg ggtcgtagcc tttggccaga atagccttca
    50941 cttgttccac ggtatgacca aaaataaaga tattttcttc accgactttc tcggcgattt
    51001 caacgttcgc accatccagc gtaccgacag taagcgcacc attgagcgcc agtttcatat
    51061 tgccggtacc ggaagcttct ttacctgcag tcgaaatttg ttcggagata tccgccgccg
    51121 ggatcatttt ttccgccgcc gaaacgcaat aatccggcag gaacaccacc ttcaacttat
    51181 cgccaaccag tggatcgttg ttgatcacgt cagccacttt gttgatcgca aagataatat
    51241 tcttcgccag gtagtagccc ggtgccgctt tcgcgccgaa gaggaagacg cgcggtacgc
    51301 gatcagcctg cgggttttca cgaatttctt tgtacaacgc cagaatatgc agcagattca
    51361 ggtgctggcg tttgtactca tgcagacgtt tgatctgaat atcgaaaatc gcctgtggat
    51421 tgatctcaat accggtacga actttcacaa actccgccag acggacttta ttcgcctgct
    51481 tgatctcgcg atattgctga cggaatttcg catcatcagc gaatttttcc agattgatca
    51541 gctgatcgag atcgttagcc cactcttttt gcagtgattt atccaacaga gccgccagtg
    51601 cagggttgca ctgtttgatc cagcgacgtg gggtaatacc gttggtgacg ttatggaatt
    51661 tgttcggcca tagctggtga tattccggga acagatcttt caccaccaga tccgagtgca
    51721 gcgccgcaac accgttcacc gcgaaaccgc caaccacaca caggttcgcc atatgcactt
    51781 gtttatcgtg caccaccgcc agtttggccc acactttttc atcgcccggc caggttttct
    51841 ccaccagcgt tttaaagcga gtattaattt cgttaataat ctgcatatgg cgcggcagta
    51901 agcctttcac cagtttcaca tcccagcgtt ccagcgcttc tggcatcagg gtatggttgg
    51961 tgtaagcgaa agttttgctg gtaatggccc aggcgtcatc ccagctcatc tggtgctcat
    52021 cgatcagcac gcgcagcagt tctggaatcg cgatagttgg gtgggtatcg ttcagctgaa
    52081 taacttcgta atccgccagt tcgtgcagtt tacgccccgc cagatgatgg cgacgcaaaa
    52141 tatccgctac cgaacaggca cactggaagt actgctgcat caagcgcagc tttttaccgg
    52201 cagtatggtt gtcgtttgga tagagaactt tggtcagttt ttccgcattg atgccctgct
    52261 gttcggcacg caggaaatca ccgtcgttaa atttagtcag atcaaacgga tgcgcgtgcg
    52321 tcgcctgcca cagacgcagc ggctgcgcca cgccattacg atagccgaca acggggagat
    52381 cccacgcttg accggtaatg gtaaactccg gctcccagcg tccgtctttc gtcactttac
    52441 cgccaatccc tacctgcaca tccagtgctt cgttgtggcg gaaccacggg tagttactgc
    52501 gatgccagtc atccggcgct tcaacctgtt tgccatcgac aaaagactgg cggaacaaac
    52561 catattgata gttcagaccg taacccgtcg cagactgacc gacagttgcc attgagtcga
    52621 ggaagcacgc cgccagacgt cccagaccac cgttacccag cgccgggtcg atctcttctt
    52681 ccagcaggtc cgtcagattg atgtcataag ccttcaacga atcctgtaca tcctgatacc
    52741 agccgagatt caacaggttg ttgcccgtca ggcgaccaat caaaaactcc attgagatgt
    52801 agttaacatg tcgctgattc gccaccggct tggcgaatgg ctgagcacgc agcatttcgg
    52861 ccagtgcttc actcactgct agccaccact ggcgaggagt catttcagcc gcagaattta
    52921 agccataacg ctgccactga cgtgaaagcg cttcctgaaa ttgcttatcg ttaaaaatag
    52981 gttgtgacat aggagttcca cttttcttag attttcaaca caacgttatc gctagtttgc
    53041 caggctcgat gttgaccttc ctcatcctgc gggggattag gcagggagga gttgcgggga
    53101 tgagcaagga aatgtgatct caaccactta aagctagtgc aaaccacagg attagcagca
    53161 aatcaatgca atacagcgca gaaaatctgt atctaagtgc aaaaaatggc cgttgcgtat
    53221 tttcaaaaag cggaaggtaa ctctataaat taagtaaagg agtgaaacag tttcataagt
    53281 aaaatatcca gtgtgctcca tctcattctt aatagattta ttaagatcat ctttttagat
    53341 ggcactttca tcaggaatga agaagaaacc cttgcttaaa tgaatctgat gaacataagg
    53401 gaaaccagta ttcacgctgg atcagcgtcg ttttaggtga gttgttaata aagatttgga
    53461 attgtgacac agtgcaaatt cagacacata aaaaaacgtc atcgcttgca ttagaaaggt
    53521 ttctggccga ccttataacc attaattacg aagcgcaaaa aaaataatat ttcctcattt
    53581 tccacagtga agtgattaac tatgctgatt ccgtcaaaac taagtcgtcc ggttcgactc
    53641 gaccataccg tggttcgtga gcgcctgctg gctaaacttt ccggcgcgaa caacttccgg
    53701 ctggcgctga tcacgagtcc tgcgggctac ggaaagacca ccctcatttc ccagtgggcg
    53761 gcaggcaaaa acgatatcgg ctggtactcg ctggatgaag gtgataacca gcaagagcgt
    53821 ttcgccagct atctcattgc cgccgtgcag caggcaacca acggtcactg tgcgatatgt
    53881 gagacgatgg cgcaaaaacg gcaatatgcc agcctgacgt cactcttcgc ccagcttttc
    53941 attgagctgg cggaatggca tagcccactt tatctggtca tcgatgacta tcatctgatc
    54001 actaatccag tgatccacga gtcaatgcgc ttctttattc gccatcaacc agaaaatctc
    54061 accctggtgg tgttgtcacg caaccttccg caactgggca ttgccaatct gcgtgttcgt
    54121 gatcaactgc tggaaattgg cagtcagcaa ctggcattta cccatcagga agcgaagcag
    54181 ttttttgatt gccgtctgtc atcgccgatt gaagctgcag aaagcagtcg gatttgcgat
    54241 gacgtttccg gttgggcgac ggcactacag ctaatcgccc tctccgcccg gcagaatacc
    54301 cactcagccc ataagtcggc acgccgcctg gcgggaatca atgccagcca tctttcggat
    54361 tatctggtcg atgaggtttt ggataacgtc gatctcgcaa cgcgccattt tctgttgaaa
    54421 agcgccattt tgcgctcaat gaacgatgcc ctcatcaccc gtgtgaccgg cgaagaaaac
    54481 gggcaaatgc gtctcgaaga gattgagcgt caggggctgt ttttacagcg gatggatgat
    54541 accggcgagt ggttctgcta tcacccgctg tttggcaact tcctgcgcca gcgctgccag
    54601 tgggaactgg cggcggagct gccggaaatc caccgtgccg ccgcagaaag ctggatggcc
    54661 cagggatttc ccagcgaagc gattcatcat gcgctggcgg caggcgatgc gctgatgctg
    54721 cgcgatattc tgcttaatca cgcctggagt ttgttcaacc atagcgaact gtcgctgctg
    54781 gaagagtcgc ttaaggccct gccgtgggac agtttgctgg aaaatccgca gttagtgtta
    54841 ttgcaggcgt ggctaatgca aagccaacat cgctatggcg aagttaacac cctgctagcc
    54901 cgtgctgaac atgaaatcaa ggacatcaga gaaggcacca tgcacgcaga atttaacgct
    54961 ctgcgcgccc aggtggcgat taacgatggt aatccggatg aagcggaacg gctggcaaaa
    55021 ctggcactgg aagagctgcc gccgggctgg ttctatagcc gcattgtggc aacctcggtg
    55081 ctgggtgaag tgctgcactg caaaggcgaa ttgacccgct cactggcgct aatgcagcaa
    55141 accgaacaga tggcacgcca gcacgacgtc tggcactacg ccttatggag tttaatccag
    55201 caaagtgaaa ttctgtttgc ccaagggttc ctgcaaaccg cgtgggaaac acaggaaaaa
    55261 gcgttccagc tgatcaacga gcagcatctg gaacagctgc cgatgcatga gtttctggtg
    55321 cgcatccgag cacaactgtt atgggcctgg gcacggctgg atgaagccga agcgtcggcg
    55381 cgtagcggga ttgaagtctt gtcgtcttat cagccacagc aacagcttca gtgcctggca
    55441 atgttgattc aatgctcgct ggcccgtggt gatttagata acgcccgtag ccagctgaac
    55501 cgtctggaaa acctgctggg gaatggcaaa tatcacagcg actggatctc taacgccaac
    55561 aaagtccggg tgatttactg gcaaatgacc ggcgataaag ccgccgctgc caactggttg
    55621 cgtcatacgg ctaaaccgga gtttgcgaac aaccacttcc tgcaaggtca atggcgcaac
    55681 attgcccgcg cacaaatctt gctgggcgag tttgaacctg cggaaattgt tctcgaagaa
    55741 ctcaatgaaa atgcccgcag tctgcggttg atgagcgatc tcaaccgtaa cctgttgctg
    55801 cttaatcaac tgtactggca ggccggacgt aaaagtgacg cccagcgcgt gttgctggac
    55861 gcattaaaac tggcgaatcg caccggattt atcagccatt ttgtcatcga aggcgaagcg
    55921 atggcgcaac aactgcgcca gctgattcaa ctcaatacgc tgccggaact ggaacagcat
    55981 cgcgcacagc gtattctgcg agaaatcaat caacatcatc ggcataaatt cgcccatttc
    56041 gatgagaatt tcgttgaacg tctgctaaat catccggaag tgccagaact gatccgcacc
    56101 agcccgttga cccaacgtga atggcaggtc ctggggctga tttactccgg ttacagcaat
    56161 gaacaaattg cgggcgaact ggaagtggcg gcaaccacca tcaagacgca tatccgcaat
    56221 ctgtaccaaa aactcggcgt cgcccaccgt caggctgccg tgcagcacgc gcagaaactg
    56281 ctgaagatga tgggttatgg ggtgtgagtt tagccggata atgcattgga tccggtttac
    56341 atctttacat cattcaatac tcacccgcgt tacgccatct gtttctatca aaccaaaccg
    56401 caccggcaag aaacgctcca ccaccgcgat attggtcagc agatggcatg aaggatgggc
    56461 gaccttaaat tctcccgcgc ccgccagcgc catcggcagt accagttggt cggcgagata
    56521 ttcccccacc gccgctgggc ttgccaggta gcgtttcacc tctttcacta actgcgcggc
    56581 taccacctcg gcactgacgc gcttttcacc gacgacgaaa aagcgttcag tgatattttc
    56641 actttcgact tccagcgaga cggtattacc cggcccctgg tcgcgcggca ggttatgaat
    56701 attctgttca tgcagggaaa aactccccgc cagtgtagcg atttcacgct cagcaacatg
    56761 gcgcggcaca ccagctaata gaacttctcc acgcatctgc acaatgttcc cgcgctcgcc
    56821 aagttgcaag gtgttaaacg atgccacggg cgagacttcc gttgccacca cgccgcctcc
    56881 ggcaggataa aaaccgtgac gtaacagcgt ggtttgctga tgaattccta ttttcgccag
    56941 cagcggctcc agcacccggc ggataaaatc cgcaggcggg gccgacgggt tatcggtgcc
    57001 tccgctcact tcaacacgcg aaggtccatc ggcaaaccac agcgcgggca gcaccgtttg
    57061 cagcaccagc gtacagcttc cggcactacc gatagcaaag cggtactcgc cgccgcgcac
    57121 ggtgccaggc cggaagacca gacgctgcga ccccagctcc gcgccttcca ccgttgccct
    57181 acaaatttcc gcagccgctt ttaccgcggt cagatgctgg cgcaacagcc ccggtttcgc
    57241 ccgcccggca cgaatgctgg tgatggtaaa tggctggccg gttatcatcg acaggctcag
    57301 cgccgagcgc aggatctgcc cgccaccttc gccctgtgcg ccatccagcg caatcatcct
    57361 tttcatcatt tatcctttta cgcacaccac ctgacgcagg gtatagataa cttccaccag
    57421 atcgctttgt gccgccatca ccgcatcaat atctttatac gccatcggga tttcgtcgat
    57481 cacttcggca tctttacggc attccacatg cgcggtggca cgaatttgat cttccacgct
    57541 gaacagtttt ttcgctttag tacggctcat tacccgcccg gcaccgtggc tgcacgaaca
    57601 gaacgactct tcatttccca gcccacggac gataaagctt tttgctccca tcgaaccggg
    57661 aataattcca tattgaccag cacgcgcaga caccgcgcct ttacgcgtca cgtagatctc
    57721 ttcaccaaag tgctgttctt tttgcacata gttgtggtga cagttgatct cttccatcgc
    57781 cagcgtttgt ggctgtctga ccgttttctg cgtaatgctc tgcaacgccg ttaccacgtt
    57841 ttccatcatc gcatcgcggt taaggctggc gaaaagctgc gcccaggcca cggctcttag
    57901 gtaatcatca aagtattccg taccttccat aaagtacgcc aggtcgcgcg atggcaacgt
    57961 ttcaagctgg tcctgcatct ctttttgtgc caaatcgata aagtaagtcc cgatggcgtt
    58021 accaattccg cgtgaaccgg agtgcagcat aatccacacc tggtccgact catcaaggca
    58081 gatttcaata aagtggttac ccgttcccag cgttcccagg tgtttatagt tgttagtgtt
    58141 aaggaaacgc ggatattttt gcgttaacca ctgataaccg gcttcaagct cagcccattt
    58201 cgcatcgacg ttaacaggcg ggttttccca tgcgccttta tcacgtttac aacggccagt
    58261 ggtacgtccg tgcggcaccg cagtttcaat cgcctgacgc agctctgcca ggttttcagg
    58321 caggtctgcc gccgttaacg cggtacgcag cgcgttcatt ccacagccaa tatccacgcc
    58381 caccgccgcc ggaataatcg cccctttggt cgggatcacg ctaccaatgg tggaaccttt
    58441 acccaggtgt acatcaggca ttaccgcaat atgtttgaaa ataaacggca tcttcgccgt
    58501 attaataagt tgctgacgcg catcggcttc taccggcacg cctttggtcc acatttttac
    58561 cggggcattt tcagtggtca gtaattcgta attcattttg tttttctctt ttcgttgttt
    58621 gct
//
LOCUS       DAZGJW010000011        58617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZGJW010000011 DAZGJW010000000
VERSION     DAZGJW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.011"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(54..533)
                     /gene="folK"
                     /locus_tag="ACHKIK_000733"
     CDS             complement(54..533)
                     /gene="folK"
                     /locus_tag="ACHKIK_000733"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO9081961.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(530..1927)
                     /gene="pcnB"
                     /locus_tag="ACHKIK_000734"
     CDS             complement(530..1927)
                     /gene="pcnB"
                     /locus_tag="ACHKIK_000734"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO9081962.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1987..2883)
                     /gene="gluQRS"
                     /locus_tag="ACHKIK_000735"
     CDS             complement(1987..2883)
                     /gene="gluQRS"
                     /locus_tag="ACHKIK_000735"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO9081963.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2950..3405)
                     /gene="dksA"
                     /locus_tag="ACHKIK_000736"
     CDS             complement(2950..3405)
                     /gene="dksA"
                     /locus_tag="ACHKIK_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO9081964.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3583..4287)
                     /gene="sfsA"
                     /locus_tag="ACHKIK_000737"
     CDS             complement(3583..4287)
                     /gene="sfsA"
                     /locus_tag="ACHKIK_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO9081965.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4302..4832)
                     /gene="thpR"
                     /locus_tag="ACHKIK_000738"
     CDS             complement(4302..4832)
                     /gene="thpR"
                     /locus_tag="ACHKIK_000738"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO9081966.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4906..7335
                     /gene="hrpB"
                     /locus_tag="ACHKIK_000739"
     CDS             4906..7335
                     /gene="hrpB"
                     /locus_tag="ACHKIK_000739"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO9081967.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKDLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7467..10001
                     /gene="mrcB"
                     /locus_tag="ACHKIK_000740"
     CDS             7467..10001
                     /gene="mrcB"
                     /locus_tag="ACHKIK_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO9081968.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10221..12464
                     /gene="fhuA"
                     /locus_tag="ACHKIK_000741"
     CDS             10221..12464
                     /gene="fhuA"
                     /locus_tag="ACHKIK_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO9081969.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLSGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12515..13312
                     /gene="fhuC"
                     /locus_tag="ACHKIK_000742"
     CDS             12515..13312
                     /gene="fhuC"
                     /locus_tag="ACHKIK_000742"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO9081970.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13312..14202
                     /gene="fhuD"
                     /locus_tag="ACHKIK_000743"
     CDS             13312..14202
                     /gene="fhuD"
                     /locus_tag="ACHKIK_000743"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO9081971.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14199..16181
                     /gene="fhuB"
                     /locus_tag="ACHKIK_000744"
     CDS             14199..16181
                     /gene="fhuB"
                     /locus_tag="ACHKIK_000744"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO9081972.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVHAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16216..17496)
                     /gene="hemL"
                     /locus_tag="ACHKIK_000745"
     CDS             complement(16216..17496)
                     /gene="hemL"
                     /locus_tag="ACHKIK_000745"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO9081973.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17662..17709
                     /gene="yadX"
                     /locus_tag="ACHKIK_000746"
     CDS             17662..17709
                     /gene="yadX"
                     /locus_tag="ACHKIK_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO9081974.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17721..19142
                     /gene="clcA"
                     /locus_tag="ACHKIK_000747"
     CDS             17721..19142
                     /gene="clcA"
                     /locus_tag="ACHKIK_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO9081975.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDGARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAMQLARSKAASASENT"
     gene            19166..19231
                     /gene="yadW"
                     /locus_tag="ACHKIK_000748"
     CDS             19166..19231
                     /gene="yadW"
                     /locus_tag="ACHKIK_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO9081976.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19224..19568
                     /gene="erpA"
                     /locus_tag="ACHKIK_000749"
     CDS             19224..19568
                     /gene="erpA"
                     /locus_tag="ACHKIK_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO9081977.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19615..20238)
                     /gene="yadS"
                     /locus_tag="ACHKIK_000750"
     CDS             complement(19615..20238)
                     /gene="yadS"
                     /locus_tag="ACHKIK_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO9081978.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20276..21076)
                     /gene="btuF"
                     /locus_tag="ACHKIK_000751"
     CDS             complement(20276..21076)
                     /gene="btuF"
                     /locus_tag="ACHKIK_000751"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO9081979.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21069..21767)
                     /gene="mtnN"
                     /locus_tag="ACHKIK_000752"
     CDS             complement(21069..21767)
                     /gene="mtnN"
                     /locus_tag="ACHKIK_000752"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO9081980.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIEKRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21851..23368
                     /gene="dgt"
                     /locus_tag="ACHKIK_000753"
     CDS             21851..23368
                     /gene="dgt"
                     /locus_tag="ACHKIK_000753"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO9081981.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23498..24922
                     /gene="degP"
                     /locus_tag="ACHKIK_000754"
     CDS             23498..24922
                     /gene="degP"
                     /locus_tag="ACHKIK_000754"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO9081982.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLGDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            25079..26230
                     /gene="cdaR"
                     /locus_tag="ACHKIK_000755"
                     /pseudo
     CDS             25079..26230
                     /gene="cdaR"
                     /locus_tag="ACHKIK_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26319..26705)
                     /gene="yaeH"
                     /locus_tag="ACHKIK_000756"
     CDS             complement(26319..26705)
                     /gene="yaeH"
                     /locus_tag="ACHKIK_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO9081983.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26867..27679)
                     /gene="yaeI"
                     /locus_tag="ACHKIK_000757"
     CDS             complement(26867..27679)
                     /gene="yaeI"
                     /locus_tag="ACHKIK_000757"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFO9081984.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSAGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLVLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27734..28558)
                     /gene="dapD"
                     /locus_tag="ACHKIK_000758"
     CDS             complement(27734..28558)
                     /gene="dapD"
                     /locus_tag="ACHKIK_000758"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO9081985.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28589..31261)
                     /gene="glnD"
                     /locus_tag="ACHKIK_000759"
     CDS             complement(28589..31261)
                     /gene="glnD"
                     /locus_tag="ACHKIK_000759"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO9081986.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31323..32117)
                     /gene="map"
                     /locus_tag="ACHKIK_000760"
     CDS             complement(31323..32117)
                     /gene="map"
                     /locus_tag="ACHKIK_000760"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO9081987.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHTVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32485..33210
                     /gene="rpsB"
                     /locus_tag="ACHKIK_000761"
     CDS             32485..33210
                     /gene="rpsB"
                     /locus_tag="ACHKIK_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO9081988.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33468..34319
                     /gene="tsf"
                     /locus_tag="ACHKIK_000762"
     CDS             33468..34319
                     /gene="tsf"
                     /locus_tag="ACHKIK_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO9081989.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34466..35191
                     /gene="pyrH"
                     /locus_tag="ACHKIK_000763"
     CDS             34466..35191
                     /gene="pyrH"
                     /locus_tag="ACHKIK_000763"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO9081990.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35483..36040
                     /gene="frr"
                     /locus_tag="ACHKIK_000764"
     CDS             35483..36040
                     /gene="frr"
                     /locus_tag="ACHKIK_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO9081991.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36132..37328
                     /gene="ispC"
                     /locus_tag="ACHKIK_000765"
     CDS             36132..37328
                     /gene="ispC"
                     /locus_tag="ACHKIK_000765"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO9081992.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37517..38275
                     /gene="ispU"
                     /locus_tag="ACHKIK_000766"
                     /gene_synonym="uppS"
     CDS             37517..38275
                     /gene="ispU"
                     /locus_tag="ACHKIK_000766"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO9081993.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNSGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38288..39145
                     /gene="cdsA"
                     /locus_tag="ACHKIK_000767"
     CDS             38288..39145
                     /gene="cdsA"
                     /locus_tag="ACHKIK_000767"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO9081994.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39157..40509
                     /gene="rseP"
                     /locus_tag="ACHKIK_000768"
     CDS             39157..40509
                     /gene="rseP"
                     /locus_tag="ACHKIK_000768"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO9081995.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40539..42971
                     /gene="bamA"
                     /locus_tag="ACHKIK_000769"
     CDS             40539..42971
                     /gene="bamA"
                     /locus_tag="ACHKIK_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO9081996.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43093..43578
                     /gene="skp"
                     /locus_tag="ACHKIK_000770"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43093..43578
                     /gene="skp"
                     /locus_tag="ACHKIK_000770"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO9081997.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43582..44607
                     /gene="lpxD"
                     /locus_tag="ACHKIK_000771"
     CDS             43582..44607
                     /gene="lpxD"
                     /locus_tag="ACHKIK_000771"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO9081998.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44712..45167
                     /gene="fabZ"
                     /locus_tag="ACHKIK_000772"
     CDS             44712..45167
                     /gene="fabZ"
                     /locus_tag="ACHKIK_000772"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO9081999.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45171..45959
                     /gene="lpxA"
                     /locus_tag="ACHKIK_000773"
     CDS             45171..45959
                     /gene="lpxA"
                     /locus_tag="ACHKIK_000773"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO9082000.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45959..47107
                     /gene="lpxB"
                     /locus_tag="ACHKIK_000774"
     CDS             45959..47107
                     /gene="lpxB"
                     /locus_tag="ACHKIK_000774"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO9082001.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47104..47700
                     /gene="rnhB"
                     /locus_tag="ACHKIK_000775"
     CDS             47104..47700
                     /gene="rnhB"
                     /locus_tag="ACHKIK_000775"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO9082002.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47737..51219
                     /gene="dnaE"
                     /locus_tag="ACHKIK_000776"
     CDS             47737..51219
                     /gene="dnaE"
                     /locus_tag="ACHKIK_000776"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO9082003.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNRALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51232..52191
                     /gene="accA"
                     /locus_tag="ACHKIK_000777"
     CDS             51232..52191
                     /gene="accA"
                     /locus_tag="ACHKIK_000777"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO9082004.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52290..54431
                     /gene="ldcC"
                     /locus_tag="ACHKIK_000778"
     CDS             52290..54431
                     /gene="ldcC"
                     /locus_tag="ACHKIK_000778"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO9082005.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVALGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54488..54877
                     /gene="yaeR"
                     /locus_tag="ACHKIK_000779"
     CDS             54488..54877
                     /gene="yaeR"
                     /locus_tag="ACHKIK_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO9082006.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54942..56240
                     /gene="tilS"
                     /locus_tag="ACHKIK_000780"
     CDS             54942..56240
                     /gene="tilS"
                     /locus_tag="ACHKIK_000780"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO9082007.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56289..56549)
                     /gene="rof"
                     /locus_tag="ACHKIK_000781"
     CDS             complement(56289..56549)
                     /gene="rof"
                     /locus_tag="ACHKIK_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO9082008.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56536..56736)
                     /gene="yaeP"
                     /locus_tag="ACHKIK_000782"
     CDS             complement(56536..56736)
                     /gene="yaeP"
                     /locus_tag="ACHKIK_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO9082009.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56902..57447
                     /gene="yaeQ"
                     /locus_tag="ACHKIK_000783"
     CDS             56902..57447
                     /gene="yaeQ"
                     /locus_tag="ACHKIK_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO9082010.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57444..57866
                     /gene="arfB"
                     /locus_tag="ACHKIK_000784"
     CDS             57444..57866
                     /gene="arfB"
                     /locus_tag="ACHKIK_000784"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO9082011.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57880..58590
                     /gene="nlpE"
                     /locus_tag="ACHKIK_000785"
                     /gene_synonym="cutF"
     CDS             57880..58590
                     /gene="nlpE"
                     /locus_tag="ACHKIK_000785"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO9082012.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
BASE COUNT        13768 a        14548 c        16317 g        13984 t
ORIGIN      
        1 aaagagagga tgtatgattt tcagaaaata tttatattcg taatataaat aaattaccat
       61 ttgcttaatt tgtcaaatgc tcttgtatgt aagacttcac gcaacgtctc cccatcagga
      121 aacgccagct ccggtgcaat ctcaaacagc ggccacagca taaatccacg attcttcata
      181 tcgtagtgcg gaacggtcag gcgttcggta tttatcactt cattaccaaa cagcatgata
      241 tcgagatcca gcgtgcgtgg tccccagcgt tcagctttac gaacacgacc ctgttgcagt
      301 tctatgcgct gtgtatggtt gagtaactct tccggggcga gcgaagtttc aagcgccacg
      361 gcggcgttga ggtaatcggg ttgatcttgc ggccccagcg gtggggtgcg gtaaaacgaa
      421 gaaacggcaa gaatgtggct ttcagggata tcgcctaatg ctttcagggc agcattgacc
      481 tgctccagcg gagaggccag attgctgcct atggcaatat acgccactgt catgcggtac
      541 cctcacgacg cggtgcgcgt ttgcgtggac gacgagtacg gcgacgcggt gacggctctt
      601 catccaactc gttaagcatc cctttttgat ctggtggcgc ggaaacctgg aactcacccc
      661 accatttcac cagacgctgc agttcagcgt tacgttcaac ttcagctcgc aaggccaaca
      721 ggtcataagc cgcacggaac ttaggatgct ccagcagttt ccatgcgcgt ttaccctgac
      781 gacgggacat acgcaactgc aactgccaga tatcgcgggt taacgtcgtc agacgtttcg
      841 ggattgccag tgaacggcag gcttcgtcca gcacgtcgtt catcgccagc gcgaaagcgt
      901 cgtgataggt caggccgctt tcctgggcaa tcttctgtgc cgtctccagc agtgggtacc
      961 agaacatggc ggcaaacagg aacgccgggt tcacgcgcat atcgttatgg atacgcgtat
     1021 cggtattctt cagcacctgt tcaatgatcc gctccatcgg gctgtcgcca ttttccgtga
     1081 agtagcgggt aatggtcggg aacagcggct ggaacagatg atattcacac aacagcttat
     1141 aggtatcgta accgtagccc gcttgtagca gtttaagcga ttcttcaaac aggcgtgccg
     1201 gtgggatatc gttcaacagg gtagcgaggc gagggatcgg ttctgcggtt tccgggctga
     1261 tgcgcatacc caatttggcg gcaaaacgta ccgcgcgcag catacgtacc ggatcttcac
     1321 ggtagcgcgt ttccgggtta ccaatcagac ggataacgcc gtccttcaga tccttcatac
     1381 cgccaacgta atcacggacg gtaaaatccg ctacgctgta atagaggctg ttgatagtga
     1441 aatcgcggcg ctgggcgtct tcttcgatgg agccgaaaat gttgtcgcgc agcaacatgc
     1501 cgttttgccc gcgttgggag gtcgtgcggt cgctgacgtt accttcgtgg tgtccacgga
     1561 aggtcgcaac ttcgataatc tccgggccaa acattacatg agccagacgg aaacggcgac
     1621 ccaccaggcg gcagttacgg aacagtttgc gcacctgctc aggcgtggcg ttagtggtta
     1681 cgtcaaaatc tttcggcttt ttgccaagta acaggtcgcg cacgccgccg ccaaccagcc
     1741 aggcttcgta tcccgctttg ttgagcctgt acattacctt cagggcattt tcactgatat
     1801 ctttgcggga aatagcatgc tgctcacgcg ggatcaccgt cacctgtgga cgggcgactg
     1861 cctgttcagc ctcgctttcc tcgcggctta gcaccttgcg gcaaaaatta gcgactcggg
     1921 taaaaatagt acacctcggt agtgtcaaac atcattcagg acaaaaaaat agcggctaat
     1981 catagctcag catgacgcat ttgagaatgt tgaatttaca attgccgact cgggcacggc
     2041 ggtaagccgc cagtttttga cggctgactg aaggatttgc tcgacgctga aatcctgcca
     2101 gtgtgcttct gcctgctgcc ccagaaattg aagtgccgcg attagtaccg ggcgtggatc
     2161 gcctttcggc aacgcaggcg catgattctg cttggaaagt ttagcgcctt gtggattaag
     2221 cgccagcggc agatgaatgt aatctggcac tttccagcca aaaagctggt acagcgagat
     2281 ttgccttact gttggttcaa tcagatcagc cccacgcact atttctgtaa cgccctggaa
     2341 atgatcatca accacaacag ccaggttgta ggcgaacaac ccatcacggc gatgaatgat
     2401 aaaatcttcc cgtgccagtt tttcgtcggc gtgaataatg ccgcgcagct ggtcagtaaa
     2461 ttgcgtgacc ggatgctgct ggcggatacg cactgcggca ttgtctggtc catgatgcaa
     2521 cacccggcaa tgaccgtcgt aaataccgcc aatgctttga atacgcgcac gcgtacaggt
     2581 gcagtaataa cttagtcctt gttcatgtaa ccaggcgagt gcttcacgat aggcgtggtg
     2641 acgttgcgat tgccagagaa catcgccgtc ccagtgcaga ccgtaatgtt ccagctggcg
     2701 caggatagtt tctgcggcac cgggaacttc acgaggcggg tcgatatctt ctatgcgtac
     2761 cagccagcga ccttgccggg cgcgagcctg caaatagctg ccgagcgcgg cgatcagaga
     2821 gccaaaatga agctcgccgg aaggagaggg ggcgaagcgg ccaatatact gtgtgtctgt
     2881 catctctttg aacaaaaaat aaggcgggag catttcccgc ctgtggtaaa cgtgatggaa
     2941 cggctgtaat tagccagcca tctgtttttc gcgaatttca gccagcgttt tgcagtcgat
     3001 gcacagatcg gctgtcgggc gcgcttccag acggcgaata ccaatttcaa caccgcagga
     3061 ttcgcagtag ccgaaatctt cgtcttccac ttttttcagc gtcttctcga tctttttgat
     3121 cagcttacgc tcgcgatcgc ggttacgcag ttcgaggctg aactcttctt cctgggctgc
     3181 acggtctacc gggtccggga agttggctgc ttcatcctgc atatgtgtaa cggtgcgatc
     3241 gacttcatcc ctgagttgat tacgccatgc ttccagaata cgacggaagt gcgccagctg
     3301 ggcttcattc atatactctt cgcccggctt ctcctgatat ggttccaccc cagcgatggc
     3361 gagaatactc agggacgatg ttttacggtt ttgcccttct tgcatgttgc ttctccttaa
     3421 cacgcactat cgatccccat gttcggggga aaaatgaggc cgctataaat agcagatgct
     3481 tttccggata gcaattatct aaacgtaaca cttgacaact gtgtgaggaa aagcgtattt
     3541 gcgcacgcga ccagaatgta aattaaccag ttacttactt tactacaatg taaccggcag
     3601 tgatttttta agagccatgc cttcagcaga aatttccgct ttgtaagcca gaatttctac
     3661 ccccctctgt tgagcttctg acaatagttg cgcgtatttc tcatcgatgt ggcgcgcggg
     3721 tgaaaaccgt gtaatggctg aatgcagcac ggcgaaaaag ataaccgcac gctggccttc
     3781 agccgctacg ctcatcaact cccgaaggtg tttctgacct cgttcagtga ccgcatcggg
     3841 aaaatatccc tgttcgttct ccgctaacgt aaccgatttc acttcaatat agcagtctgg
     3901 acgcgaatcc gcctgcaaca taaagtcaat acggctgcgt tctgcgccgt attttacttc
     3961 gcttttcagc gagctatagc ctgacagttc tgaaattgat tcattaagga tagcctcttt
     4021 cgtcaacctg ttagcccaaa gcgtgttgac gcaaataaat gcgccgctct ggctttgagt
     4081 taattcccag gtgtgtgggt atttccgttt ggtgttgtct gaagtcgaat accagacggt
     4141 atcgccaggc gttgcacaac cggtcattgc acccgtattc gggcagtgta gcgttaattc
     4201 gcgaccatcg ggtgtgatca catcggctaa aaaacgtttg taacgctgaa ttagcgtcgc
     4261 gcgctgtaga gggggagaaa attccattgc gacaatcctt gttattgcgt tagcgcccag
     4321 cgttttagcg gcgtgtagcg tgtgcgtcca cgggcaaacg aggaggcgta aagggtgaac
     4381 tccgtcaccg catacgacca gttaaaacct ggcggcggga ttgtcaccgc ctcgctggcg
     4441 tcgcgcaata aggtaatatg tggatgaaac ggacgattgc tttgaaaaca accgctgcgg
     4501 gcagcctgtg aacggagcat attcgccagc tggattaagc cgcgtggcgg ctgacgcatc
     4561 cctaaccaca ccacacgcga acgcagccat tgtccggcgt catcaagcgt gagtgtgaaa
     4621 ccaggttgac gaatccgtcc ggctaaaaga gaaagcgcct tctctttctc ggcgctcact
     4681 tcgcctaaaa atgccagagt cagatgcaaa ttatcggcgg cgaccggacg tcccgcctca
     4741 ggtgggaagt gtgtggcgcg ccagtggata atctgttcgc ggatttctgc aggtaagtcg
     4801 atagcaaaga acagacgttg cggttcagac atgtgaggca ctcggttatg aattaccgcg
     4861 atgctacaat gtggcgcgaa gaatgttaac cctctggagc gttttgtgtc gtcgttgccc
     4921 gttgctgccg tcttacctga attacttacc gctctcgatt gtgcgccgca ggtattatta
     4981 agtgcgccga ccggggccgg gaaatcaacc tggctgccgc tgcaactgct ggcgcatccc
     5041 ggcattaacg ggaaaattat cctgctggag ccgcgtcgtc tggcggcgcg taacgtcgcg
     5101 caacggctgg cggagctgct taacgaaaag ccaggcgata ccgttggcta ccggatgcgt
     5161 gcgcaaaact gcgtcgggcc gaatacccgc ctggaagtag ttaccgaagg cgtgctgacg
     5221 cggatgatcc agcgtgaccc ggagttgagc ggtgttggat tggtgatcct cgatgagttt
     5281 catgagcgca gcttgcaggc ggatttggcg ttggcgctgt tactcgatgt gcaacaaggt
     5341 ctgcgtgatg accttaaact gctgattatg tcggctacgc tggacaacga ccgcttgcag
     5401 caaatgctgc cagaagcgcc cgtcgtcatc tcagaagggc gctcgtttcc ggttgaacgc
     5461 cgttatttac cactgcccac gcatcagcgt tttgacgaag ccgttgcggt agccaccgct
     5521 gaaatgctgc gtcaggaaag tggatcatta ctgttatttt tacctggcgt cggagaaatt
     5581 cagcgtgtgc aggaacaact ggcttcgcgc atcggcagcg atgtattgct ctgcccgctg
     5641 tatggcgcgt tgtcgctgaa cgatcagcga aaagcgatcc tcccggcacc gcaagggatg
     5701 cgcaaagtgg tgctggcgac caatattgct gaaaccagtt taaccatcga aggtattcgt
     5761 ctggtggtgg attgtgccca ggagcgtgtg gcgcgttttg atccgcgcac ggggcttacg
     5821 cgactgatta ctcaacgcgt tagccaggca tccatgacgc agcgtgccgg gcgcgccggg
     5881 cgtctggagc cgggtatctg cctgcattta atcgccaagg aacaagcaga acgcgccgcg
     5941 gcgcaaagtg aaccggagat attacaaagc gatctttccg gtttgctgat ggaattactg
     6001 caatggggat gcagcgatcc ggcgcagatg agctggctgg atcaaccgcc agtagtgaat
     6061 ctactggccg cgaaacgtct gttacaaatg ctgggggcac tggagggtga acggcttagt
     6121 gcgcaagggc aaaaaatggc agcgctgggt aacgatccgc gtttagcggc aatgctggtt
     6181 agcgcgaaga acgacgacga agctgctacc gcggcaaaaa ttgccgccat tctcgaagag
     6241 ccgccacgga tgggcaatag tgacctgggc gtggcgtttt cgcgcaatca accagcctgg
     6301 cagcaacgta gtcagcaact gttaaaacgc ttaaacgtac gtggcggtga ggcagacagt
     6361 tcgcttatcg cgccgctact tgccggggcg tttgccgatc gcattgctcg tcgccgtggg
     6421 caagatggac gctatcaact ggcaaacggc atgggagcga tgctcgatgc caacgacgcg
     6481 ctaagccgcc acgaatggtt gatcgcaccg ttattattgc agggcagcgc ctcgccggat
     6541 gcgcggattt tactggcgct gccggtcgat attgatgagt tagtacaacg ctgcccgcag
     6601 ctggtacagc agtctgacac tgtggagtgg gatgacgcgc aaggtacgct gaaagcctgg
     6661 cgtcggctgc aaatcggtct gctgacggtg aaagtgcagc cgctggcgaa accctcggaa
     6721 gacgagttgc atcaggcgat gcttaatggc atccgcgata aagatttaag cgtgctcaac
     6781 tggacggcgg aagcggaaca gctacgcttg cgtttgttat gcgccgcaaa gtggttgccg
     6841 gaatatgact ggccagcggt tgatgatgaa agtttattgg cgacgctgga aacgtggctg
     6901 ctgccacata tgactggcgt acattcacta cgcggcctga aatcactcga catttatcag
     6961 gcactacgcg gattacttga ttggggaatg cagcaacgtc tggatagtga attgcctgcg
     7021 cattacactg tgccgacggg aagccggatc gccattcgtt atcatgaaga taacccgccc
     7081 gcgctggcgg tgagaatgca agagatgttt ggcgaggcca ccaatccgac gatcgcccag
     7141 gggcgcgtgc cgctggtgct ggagttgctt tcacctgccc aaaggccatt acaaatcaca
     7201 cgagatttga gcgccttctg gaaaggagcg taccgtgagg tgcaaaaaga gatgaaaggg
     7261 cgttatccca aacatgtctg gccggacgac ccggcaaata ctgcaccgac gcgacggacg
     7321 aaaaagtatt cgtaaggttg gttttctccc tctccctgtg ggagagggga ccgatcgagc
     7381 acaaattttg agagatatct tcttctgtct tgtaacagaa gaacagaaaa tcgggctttt
     7441 gcgcctgaat attgcggaga aaaagcatgg ccgggaatga ccgcgagcca attggacgca
     7501 aagggaaacc gacgcgtccg gtcaaacaaa aggtaagccg tcgtcgttac gaagatgacg
     7561 atgattacga cgattatgat gactatgagg atgaagaacc gatgccgcgc aaaggtaagg
     7621 gcaaaggcaa agggcgtaag cctcgtggca aacgcggctg gctatggctg ctgttaaaac
     7681 tggctatcgt ttttgccgtg ctgatcgcca tttacggcgt ttatctcgat caaaaaattc
     7741 gtagccgtat tgatggcaag gtctggcaac tgcctgcggc agtttatggc cgaatggtca
     7801 accttgagcc agatatgacc atcagcaaga acgagatggt gaagctgctg gaggcgactc
     7861 agtatcgtca ggtgtcgaaa atgacccgtc ctggcgaatt taccgtgcag gccaacagca
     7921 ttgagatgat ccgccgtccg tttgatttcc cggacagtaa agaagggcag gtgcgtgcgc
     7981 gtctgacctt tgatggcgat catctggcga cgatcgtcaa tatggagaac aaccgtcagt
     8041 tcggtttctt ccgtcttgat ccgcgtctga tcaccatgat ctcctcgcca aacggtgagc
     8101 agcgtttgtt tgtgccgcgc agtggtttcc ctgatttgct ggtggatact ttgctggcga
     8161 cagaagaccg tcatttttac gagcatgacg gcatcagcct ctactccatc ggacgtgcgg
     8221 tgctggcaaa cctgaccgcc ggacgcacgg tgcagggagc gagtacgctg acgcaacagc
     8281 tggtgaaaaa cctgttcctc tccagtgagc gttcttactg gcgtaaagcg aacgaagctt
     8341 acatggcgct gatcatggac gcgcgttaca gcaaagaccg tattcttgag ctgtatatga
     8401 acgaggtgta tctcggtcag agcggcgaca acgaaatccg cggcttcccg ctggcgagct
     8461 tgtattactt tggtcgcccg gtagaagagc taagcctcga ccagcaggcg ctgttagtcg
     8521 gcatggtgaa aggagcgtcc atctacaacc cgtggcgtaa cccaaaactg gcgctggagc
     8581 gacgtaatct ggtgctgcgt ctgctgcaac agcaacagat tattgatcaa gaactctatg
     8641 acatgttgag tgcacgtccg ctgggtgttc agccacgcgg tggggtgatc tctccacagc
     8701 cagcctttat gcaactggtg cgtcaggagc tgcaggcgaa attaggcgat aaggtaaaag
     8761 atctctccgg cgtgaagatc ttcactacct ttgactcggt ggcccaggac gcggcagaaa
     8821 aagccgccgt ggaaggcatt ccggcactga agaaacagcg taagttgagc gatcttgaaa
     8881 ctgcgattgt ggtcgtcgac cgctttagtg gtgaagttcg tgcgatggtc ggtggttctg
     8941 agccgcagtt tgcgggctac aaccgtgcga tgcaggcgcg tcgttcgatt ggttcccttg
     9001 caaaaccagc gacttatctg actgccttaa gccagccgaa aatctatcgt ctgaatacgt
     9061 ggattgcgga tgcgccaatc gcgctgcgtc agccgaatgg ccaggtctgg tcaccgcaga
     9121 atgatgaccg tcgttatagc gaaagcggca gagtgatgct ggtggatgcg ttgacccgtt
     9181 cgatgaacgt gccgacggta aatctgggga tggcgctggg gctgcctgcg gttacggaga
     9241 cctggatcaa actgggcgta ccgaaagatc agttgcatcc ggttccggcg atgctgctgg
     9301 gggcgttgaa cttaacgcca atcgaagtgg cgcaggcgtt ccagaccatc gccagcggcg
     9361 gtaaccgtgc accgctttct gcactgcgtt cggtaattgc ggaagatggc aaggtgctgt
     9421 atcagagctt cccgcaggcg gaacgcgctg ttccggcgca ggcggcgtat ctgacactat
     9481 ggaccatgca gcaggtggta caacgcggta cgggtcgtca gcttggggcg aaatacccga
     9541 acctgcatct ggcagggaaa acagggacta ccaacaataa cgtagatacc tggtttgcag
     9601 gcattgacgg cagcacggtg accatcacct gggtaggccg tgataacaac cagccgacca
     9661 aactgtatgg agccagcggg gcaatgtcga tttatcagcg ttatctggct aaccagacgc
     9721 caacgccgct gaatcttgtt ccgccagaag atattgcaga tatgggcgtg gactacgacg
     9781 gcaactttgt ttgcagcggt ggcatgcgtg tcttgccggt ctggaccagc gatccgcaat
     9841 cgctgtgcca gcagagcgag atgcagcagc agccgtcagg caatccgttt gatcagtctt
     9901 ctcagccgca gcaacagccg caacagcaac ctgctcagca agagcagaaa gacagcgacg
     9961 gtgtagccgg ttggatcaag gatatgtttg gtagtaatta acatctaagc gtgaaatacc
    10021 ggatggcgag ttgccatccg gtaaaataac atcccatcta agatattaac cctttctttt
    10081 catctggttg tttattaacc cttcaggaac gctcagattg cgtaccgctt gcgaacccgc
    10141 cagcgtttcg aatattatct tatctttata ataatcattc tcgtttacgt tatcattcac
    10201 tttacatcag agatatacca atggcgcgtt ccaaaactgc tcagccaaaa cactcactgc
    10261 gtaaaatcgc agttgtagta gccacagcgg taagcggcat gtctgtttat gcacaggcag
    10321 cggttgaacc gaaagaagac actatcaccg ttaccgctgc acctgcgccg caagaaagcg
    10381 catgggggcc tgctgcaact attgcggcgc gacagtctgc taccggcact aaaaccgata
    10441 cgccgattca aaaagtgcca cagtctattt ctgttgtgac cgccgaagag atggcgctgc
    10501 atcagccgaa gtcggtaaaa gaagcgctta gctacacgcc gggtgtctct gttggtacgc
    10561 gtggcgcatc caacacctat gaccacctga tcattcgcgg ctttgcggca gaaggccaaa
    10621 gccagaataa ctatctgaat ggcctgaagt tgcagggcaa cttctataac gatgcggtca
    10681 ttgacccgta tatgctggaa cgcgctgaaa ttatgcgtgg cccggtttcc gtgctttacg
    10741 gtaaaagcag tcctggcggc ctgttgaata tggtcagcaa gcgtccgacc accgaaccgc
    10801 tgaaagaagt tcagtttaaa gccggtactg acagcctgtt ccagactggt tttgacttta
    10861 gcgattcgtt ggatgatgac ggtgtttact cttatcgcct gaccggtctt gcgcgttctg
    10921 ccaatgccca gcagaaaggg tcagaagagc agcgttatgc tattgcaccg gcgttcacct
    10981 ggcgtccgga tgataaaacc aatttcacct tcctttctta cttccagaac gagccggaaa
    11041 ccggttatta cggctggttg ccgaaagagg gaaccgttga gccgctgccg aacggtaagc
    11101 gtctgccgac agactttaat gaaggggcga agaacaacac ctattctcgt aatgagaaga
    11161 tggtcggcta cagcttcgat cacgaattta acgacacctt tactgtgcgt cagaacctgc
    11221 gctttgctga aaacaaaacc tcgcaaaaca gcgtttatgg ttacggcgtc tgctccgatc
    11281 cggcgaatgc ttacagcaaa cagtgtgcgg cattagcgcc agcggataaa ggccattatc
    11341 tggcacgtaa atacgtcgtt gatgatgaga agctgcaaaa cttctccgtt gatacccagt
    11401 tgcagagcaa gtttgccact ggcgatatcg accacaccct gctgtccggt gtcgacttta
    11461 tgcgtatgcg taatgacatc aacgcctggt ttggttacga cgactctgtg ccactgctca
    11521 atctgtacaa tccggtgaat accgatttcg acttcaatgc caaagatccg gcaaactccg
    11581 gcccttaccg cattctgaat aaacagaaac aaacgggcgt ttatgttcag gatcaggcgc
    11641 agtgggataa agtgctggtc accctaggcg gtcgttatga ctgggcagat caagaatctc
    11701 ttaaccgcgt tgctgggacg accgataaac gtgatgacaa acagtttacc tggcgtggtg
    11761 gtgttaacta cctgtttgat aatggtgtaa caccttactt cagctatagc gaatcgtttg
    11821 aaccttcttc gcaagttggg aaggatggta atattttcgc accgtctaaa ggtaagcagt
    11881 atgaagtcgg cgtgaaatat gtaccggaag atcgtccgat tgtagttact ggtgccgtgt
    11941 ataatctcac taaaaccaac aacctgatgg cggaccctga gggttccttc ttctcggttg
    12001 aaggtggcga gatccgcgca cgtggcgtag aaatcgaagc gaaagcggcg ctgtcggcga
    12061 gtgttaacgt agtcggttct tatacttaca ccgatgcgga atacaccacc gatactacct
    12121 ataaaggcaa tacgcctgca caggtgccaa aacacatggc ttcgctgtgg gctgactata
    12181 ccttctttga cggtccgctt tcaggtctga cgctgggcac cggtggtcgt tatactggct
    12241 ccagctatgg tgatccggct aactccttta aagtgggaag ttatacggtc gtggatgcgt
    12301 tagtgcgtta tgatctggcg cgagtcggca tggcgggctc caacgtggcg ctgcatgtta
    12361 acaacctgtt cgatcgtgaa tacgtcgcca gctgctttaa cacttatggc tgcttctggg
    12421 gcgcagaacg tcaggtcgtt gcaaccgcaa ccttccgttt ctaatttctc ttttggggca
    12481 cggatttccg tgcccatttc acaagttggc tgttatgcag gaatacacga atcattccga
    12541 taccactttt gcactgcgta atatctcctt tcgtgtgccc gggcgcacgc ttttgcatcc
    12601 gctgtcgtta acctttcctg ccgggaaagt gaccggtctg attggtcaca acggttctgg
    12661 taaatccact ctgctcaaaa tgcttggccg tcatcagcct ccgtcagaag gggagattct
    12721 tcttgatgcc cagccgctgg aaagctggag cagcaaagcg tttgcccgca aagtggctta
    12781 tttgccgcag cagcttcctc cggcagaagg gatgaccgtg cgtgaactgg tggcgattgg
    12841 tcgttacccg tggcatggcg cgctggggcg ctttggtgcg gcagatcgcg aaaaggtcga
    12901 ggaagctatc tcgctggttg gcttaaaacc gctggcgcat cggctggtcg atagtctctc
    12961 tggcggcgaa cgtcagcggg cgtggatcgc catgctggtg gcgcaggata gccgttgtct
    13021 gttgctcgac gaaccgactt cggcgctgga tatcgcccac caggttgatg tgctggcgct
    13081 ggtgcaccgt ttaagtcagg aacgtggcct gacggtcatt gccgtgctgc acgatatcaa
    13141 tatggcggca cgctactgtg attatctggt cgccctgcgc ggtggtgaaa tgattgctca
    13201 gggaacgcct gcggaaatta tgcgcggcga aaccctcgaa atgatttatg gcatcccgat
    13261 gggtattttg ccgcatccgg cgggtgctgc acctgtgagt tttgtttatt gatgagcggc
    13321 ttacctctta tttcgcgccg tcgactgtta acggcgatgg cgctttctcc gttgttatgg
    13381 cagatgaata ccgcccacgc ggcggctatt gaccccaatc gtattgtggc gctggagtgg
    13441 ttgccggtgg aattactgct ggcgctcggc atcgtgcctt acggcgtggc ggataccatc
    13501 aactatcgcc tgtgggtcag cgaaccacca ttgccggact cagtgattga cgtcggtttg
    13561 cgtacagaac ctaaccttga actgctgacc gaaatgaaac cgtcgtttat ggtctggtcg
    13621 gcaggatatg gcccttcacc agaaatgctg gcacgtattg cgccgggtcg cggatttaac
    13681 ttcagtgacg gcaaacagcc gttggcgatg gcgcgtaaat cgctgacgga aatggcagat
    13741 ttacttaacc tgcaaagcgc agcagaaacg catttagcgc actatgaaga ctttatccgc
    13801 agcatgaaac cccgctttgt gaagcgtggt gcacgcccgt tactgctgac gacgcttatc
    13861 gatccgcgcc atatgctggt cttcggtcca aacagcttgt tccaggaaat tctcgatgag
    13921 tacggcatcc caaatgcctg gcaaggggaa accaacttct ggggcagtac cgccgtcagt
    13981 atcgatcgtc tggcggcgta taaagacgtt gatgtgctct gttttgatca cgacaacagc
    14041 aaagacatgg atgcgctaat ggcaaccccg ctgtggcagg ccatgccgtt tgtccgtaca
    14101 gggcgctttc agcgcgtacc cgcagtctgg ttttatggtg caacgctttc ggcaatgcac
    14161 tttgtgcgtg ttctggataa cgccatcgga ggtaaagcgt gagtaaacga attgcgcttt
    14221 tcccggcgtt attgctggcg ctgttagtga ttgtcgctac ggcgctaacc tggatgaact
    14281 tctcacaggc gctgccgcgc agtcagtggg cgcaggccgc ctggtcgccg gatattgacg
    14341 tcatcgagca gatgattttt cactacagct tgttgccgcg tctggcgatt tcgctgctgg
    14401 tgggcgcagg cctggggctg gtgggcgtgc tgtttcagca agtgctgcgt aatccgctgg
    14461 cggaaccgac gacacttggc gttgctacag gcgcgcaact gggaattacc gtcaccacac
    14521 tttgggcgat cccaggtgct atggcgagcc agtttgccgc actggtgggc gcttgtgtcg
    14581 ttggcttaat cgtctttggc gtcgcgtggg ggaaacggct ttcgccggtt acgcttattc
    14641 tcgcggggct ggtggtgagc ctttattgcg gggcaattaa tcagctactg gttatcttcc
    14701 atcacgacca gctgcaaagc atgttcctgt ggagcaccgg aacgctgacg caaaccgact
    14761 ggggcggcgt tgagcgttta tggccgcagt tgctgggcgg cgtgatgttg acgttattgc
    14821 tacttcgccc gttaaccctg atggggcttg atgatggcgt ggcgcgcaat ctcgggctgg
    14881 ccttgtcgct cgcgcgtctg gcagcgctgt cgctggcgat tgtcatcagt gcgctgctgg
    14941 tgcacgctgt ggggattatc ggctttatcg ggttgttcgc gccgctgctg gcaaaaatgc
    15001 tgggggcgcg gcgtctgctg ccacgactga tgctggcgtc gctgattggg gcgctgatcc
    15061 tctggctttc cgatcaaatc atcctctggc tgactcgcgt gtggatggaa gtgtccaccg
    15121 gttcggtcac tgcgttgatc ggtgcgccgc tgctactgtg gctgttgccg cgtttacgca
    15181 gcattagcgc gccggatatg aaggtcaacg atcgtgtcgc ggctgaacgc caacatgtgc
    15241 tggcgtttgc cctcgcgggc ggcgtgctgc tgttgatggc tgtggtggtg gcgctgtcgt
    15301 ttggtcgtga tgcgcacggc tggacgtggg cgagcggggc gttgcttgag gatttgatgc
    15361 cctggcgctg gccgcgaatt atggcggcgc tgtttgcggg cgtcatgctg gcggtggcgg
    15421 gctgtattat tcagcggctg accggaaacc cgatggcaag cccggaagtg ctggggatta
    15481 gctctggcgc agcgtttggc gtggtgttga tgctgtttct ggtgccgggt aatgcctttg
    15541 gctggctgtt acctgcaggc agtctcggcg cggcggtgac gctgttgatc attatgatcg
    15601 ccgccggccg cggtggattt tccccacacc gcatgttact ggcggggatg gcgttaagca
    15661 ccgcgttcac catgcttttg atgatgttgc aggcaagtgg tgacccgcga atggcgcaag
    15721 tgctgacctg gatttccggt tcgacctaca acgcgaccga tgcgcaggtc tggcgcacgg
    15781 gaattgtgat ggtgattttg ctggcgatta ccccgctgtg ccgccgctgg ctgaccattt
    15841 taccgctggg tggtgatacc gcccgagccg taggaatggc gctgacgccg acgcgaattg
    15901 cgctgctgct gttagcggct tgcctgacgg cgaccgcgac gatgactatt ggaccgttga
    15961 gttttgttgg tttaatggca ccgcatattg cgcggatgat gggctttcga cggacgatgc
    16021 cacacatcgt aatttcggcg ctggtgggtg gtttactgct ggtgttcgct gactggtgtg
    16081 ggcggatggt gctgtttcca ttccagatcc cagcagggct gctgtcgacc tttatcggcg
    16141 cgccatattt tatctatttg ttgagaaagc agagccgtta atagaatgcc tcgcccggtg
    16201 ggcgaggcaa aatcatcaca acttcgcaaa cacccgtcgt gcagcatcga tggtgttatt
    16261 gatatcttcc atgctgtgcg ccacggacat aaagcccgct tcaaacgctg acggtgccag
    16321 gtaaacacct tcgtccagca tcatatggaa gaaacgctta aagcgttcca cgtcacaggc
    16381 catcacatcc tgatagcacg tcacggactc ggcgtcggta aagaaaatac cgaacatgcc
    16441 gccaacgtgg ttaacgacca gcggaattcc ggcttcttct gccgcttcca gcagaccttc
    16501 tgccagacgt gttgtcagct catccagcgt ttcgtgaacg cccggctgcg cgacttcatt
    16561 cagacaggcg aaacccgctg ccatcgcaat tgggttaccg gaaagcgtac ccgcctgata
    16621 gaccggaccc gtcggggcca gcgcatccat tacatcacga cgaccaccga atgcgcctac
    16681 cggcattcca ccgccgatga ttttgcccag gcaggtgaga tccggttcca caccgtaata
    16741 atcctgtgcg ccagctagcg ctacgcggaa gccagtcatt acttcatcga tgatcagcaa
    16801 tgcgccaaat tcgtcgcaca gcgcgcgcag acctggcagg aactctggca gcggtggaac
    16861 gcagttcata ttgcctgcca ccggctcgac gataatacag gcaatctctt gcgggtattg
    16921 ctcaaacgcg gcgcgtacag aagccagatc gttataagta caggttaagg tatgtttggc
    16981 gaaatctgcc ggaacacccg gcgagtttgg ctggcctaac gtgagtgcgc cagaaccggc
    17041 tttcaccagc aggcagtcag cgtgaccgtg gtaacaacct tcaaatttaa tgattttgtc
    17101 gcgaccggta aaaccacggg ccaggcggat ggcgctcatc gtcgcttcag tgccggagtt
    17161 caccatgcgc accatatcca tggtcgggac cagttcggtc accagttgcg ccattttcac
    17221 ttccatttcg gtcggtgcac caaagcttaa accacgctcg gcggcttcaa tcacggcatt
    17281 gcggatagcc ggatggttat ggcccagcac catcggcccc caggaaccga cataatcgat
    17341 ataggctttg ccatcaacat cgtacagata agcgccgtcc gctttttcga taaacagtgg
    17401 agtgccgccc acgccagtaa aggcgcgaac aggggagttc acaccgccag ggatcagctc
    17461 gcgcgctgcg ctgtaaagat tttcagactt actcatggag ggttcctgat tcgtagaaaa
    17521 agtgaatggc tgctattcta tgttattcat aacaagttaa atactcgtca aacatcaggc
    17581 tgcttgtact ggtgcaatcc tgatttctta gagtataaaa gttttgtgca tttgaaacat
    17641 tacgctttgc aaaggatttt catggaacgt gcgagtaaaa tgccgtcatc ttatttgtat
    17701 gaccaataag tgatcattgg atgaaaactg atactccctc tttagaaaca ccgcaggccg
    17761 cgcgcctgcg acgcagacaa ctgattcgcc aacttcttga gcgcgataaa accccgttag
    17821 ccattttgtt tatggcggca gtcgtcggca cgcttgtcgg gctggcagcg gttgcttttg
    17881 acaaaggtgt cgcctggttg cagaaccaac gtatgggggc gctggtacat actgctgata
    17941 attatccgct tctgttaacc gtcgcttttc tctgttcggc ggtgctggcg atgttcggct
    18001 actttttggt gcgcaaatac gcgccggaag caggtggttc ggggatcccg gaaattgaag
    18061 gggcgctgga agatcaacgt cccgttcgct ggtggcgtgt gttgccggtg aaattctttg
    18121 gcgggctggg gacactcggc ggaggcatgg tgctagggcg cgaaggacca accgtgcaga
    18181 tcggcggcaa tatcggtcgc atggtgcttg atattttccg cctgaaaggt gatggagctc
    18241 gccatacgct gctggcaacc ggtgctgctg cggggctggc tgcggccttt aacgcgccgc
    18301 tggcgggtat tttgtttatt atcgaagaga tgcgtccgca gtttcgctat acgttaattt
    18361 cgattaaagc ggtatttatt ggcgtcatta tgtcgaccat tatgtaccgg atttttaatc
    18421 atgaagttgc gttgattgac gtcggtaaac tttctgatgc gccgcttaat acgctgtggc
    18481 tttatctgat cctcggtatt atttttggca ttttcggccc tatttttaat aaatgggtgc
    18541 tggggatgca ggatttgctg caccgtgtgc acggcggcaa tattaccaaa tgggtactga
    18601 tgggtggtgc gattggcggt ctgtgtggat tgctggggtt tgtggcacca gcaacgtcgg
    18661 gcggcggttt taacctgatt cctatcgcta ccgcggggaa tttcagcatg ggaatgctgg
    18721 tgtttatttt cgtcgctcgg gtcattacca ccttactctg cttctcttcc ggcgcgccgg
    18781 gcggtatttt tgccccgatg ctggcgctgg gtactgtgct gggaaccgct ttcggaatgg
    18841 ttgccgttga gctgtttccg caatatcacc tggaggcggg gacgtttgct attgccggaa
    18901 tgggggcatt actggcggca tctattcgcg cgccgttaac ggggatcatt ctggttctgg
    18961 agatgaccga taactaccag ctcattttgc caatgattat taccggtctt ggcgcaacac
    19021 tattagcgca atttaccggc gggaaaccgc tatactcggc gattcttgcg cgcacgctgg
    19081 caaaacagga agctatgcaa ctggcgcgaa gcaaggccgc atcagccagc gagaatactt
    19141 gaacgaaata ccagggtatt agataatggc gattattatt gggttagaat ttgcccaatt
    19201 gccgatgtcg tttggagcaa aatatgagtg atgacgtagc actgccgctg gagtttaccg
    19261 acgcagcagc caacaaagtt aaaagcctga tcgctgacga agataacccg aatctgaaat
    19321 tacgcgtgta tatcaccggt ggcggttgca gcggcttcca gtatggtttc acctttgatg
    19381 atcaggtgaa cgaaggcgat atgaccatcg aaaaacaggg cgttggcttg gtggttgatc
    19441 cgatgagcct gcaatatctg gtcggcggtt ccgttgacta taccgaagga ctggaaggtt
    19501 ctcgtttcat cgtgaccaac ccgaacgcga aaagcacctg cggttgcggt tcttccttta
    19561 gcatctaatc tgttgttcat gtgccggata aactatccgg cacatcttct ctcttcaacg
    19621 cccattctca tccagcgcaa acgtcggtag cttaagatgc caacgaatag ccgccagccg
    19681 aatcaagagc gtcacgacca tgcccatcat actggctgtt tccagtggta cggaaaatgt
    19741 gtaataagcc gtagcgtgga caataccgcc gataatacag gcagttgcgt agatttctgt
    19801 acgtaaaatc atggggattt cgcgggccag aacatcacga atgatcccgc cgccaacgcc
    19861 agtaatgacg cccatacaaa ccgcgattaa cggaccggct tccgcattaa aggctttatt
    19921 cacgccaatg ccgacaaaca ccgccagacc aacggcgtcc agcaccggca acatccattt
    19981 tggtaagcgt cttggctggc gcaccagcac gatggtcagc atgctggtta ccattgcaac
    20041 gaccagatcg gtgggatctt tcacccaaaa taccgggccg tgatccagcg ccatgtcgcg
    20101 aattgtcccg ccgcctacag cggtaaccac gcccagtacc agaacaccaa aagggtccat
    20161 acgcaatttt ccggctaaca aaacgccgga gatggcaaat accgctgtgc cgactatatc
    20221 cagccaatag acgagcattg ttcaatcccc actgagcacc tgaaaaggtc aggcactaat
    20281 ctacctgtga aagcgcatta cagagctgtt gtgcagcgag gataatacgt gggcttgcac
    20341 gttcaaacca gtcactcgtg agaggaataa cgggaatttt gagctgttca ccccagtatt
    20401 gtttgatttt aggaatttgg tccggtccgc ctgtaatgac aatcgcctgt ggcgagcgtg
    20461 ctaacacctg ttcgcggcta acttgcggcc agggaacccg gctgtcttta aagatgtttt
    20521 ctccgccaca aacttcgagt acctggttct gaatcgactc ttttccactg gtaaatggcg
    20581 gattaatgcc gaattgcaga aaaacacgtt ttttaggttt atcagcatat tgcgctttca
    20641 attgcgcgta ctgatccagc agggattgcg cggcttgttc ggccttgtct ggttgcggac
    20701 tccagggggc cagttgacgt aacgcattgg caatttgttc aatgcttgtc gcatcgaccc
    20761 acatcacttt tattcccagc gaagccagct ggtcaacctg ccgctcggca ttacctccac
    20821 gccaggcaat caccagatcg ggtttcagcg cgacaatgcg ttccagattc atcccctgcc
    20881 aggtggaaac ctgctcaatc ttttgcgctt gtggaggata gtcggaatag ctgctgaccc
    20941 caaccggcgt gatcccggcg gcaaaggcaa gttcagtgtt ggcgggagaa agcgtgatga
    21001 cgcgcggcgc ggcgttgagc cacagtggcg caagaaaaga cagggcgacc agcgccctga
    21061 acagtgactt agccatgtgc aagtttctgc accagtgact caaccatcag gctggactgt
    21121 ttagcggcaa cagccaggaa ctcatcgaag ctaagatgag actgttgatc ggccacgtcg
    21181 gagatggcgc gtacgacaac aaacgggacg ttgaaattgt ggcagacatg ggcgattgcc
    21241 gtcgcttcca tctctacagc aatggcctgt gggaagttgt ggcggatttt cgccagacca
    21301 acagaaccgt tgatgaaagc gtcgccgcta acaatcaggc cacgtacagc gttaagattc
    21361 agttcggcaa tgcaggcctc agcggcagcg atcagtttat cgtcagcttt aaagcctgcc
    21421 ggacagcctg gtaactgacc gtattcataa ccaaatgccg tgacatccgc gtcgtgataa
    21481 cgtgcttcgt ccgagacaac gatatcgccc actttcaacg ttggtgccag gccaccggca
    21541 gaaccggtgt taataatcac atctggcttg cagtgttcca acagcaaagt ggcacccagc
    21601 gccgcagcga ctttaccgat gcccgatttc agaagcgcaa cctcggttcc attcagttgg
    21661 ccggtataga tttcgcaacc gccgagactg atagtttgac gtttttcgat tttgtcacgc
    21721 agcagcgtaa cttcttcttc cattgcacca atgatgccga ttttcataga tttactcgcg
    21781 ataagcccga tttgaaggca tagtttacca tgcgcttacg gggaagcgta tttctcacgc
    21841 gggagaggac atggcacaga ttgatttccg aaaaaaaata aactggcatc gtcgttaccg
    21901 ttcaccgcag ggcgttaaaa ccgaacatga gatcctgcgg atcttcgaga gcgatcgcgg
    21961 gcgtatcatc aactctccgg caattcgtcg tctgcaacaa aagacgcagg tttttccact
    22021 ggagcgcaat gccgccgtgc gcacgcgtct tacccactcg atggaagtcc agcaggtggg
    22081 gcgctacatc gccaaagaaa ttttaagccg tctgaaagag cttaaattgc tggaagcata
    22141 cggcctggat gaactgaccg gaccttttga aagcattgtt gagatgtcat gcctgatgca
    22201 cgatatcggc aatccgccgt ttggtcattt tggcgaagca gcgataaatg actggtttcg
    22261 ccaacgtttg cacccggaag atgccgaaag ccagcctctg actgacgatc gctgcagcgt
    22321 ggcggcacta cgtttacggg acggggaaga accgcttaac gagctgcggc gcaagattcg
    22381 tcaggactta tgtcattttg aggggaatgc acaaggcatt cgtctggtgc atacattgat
    22441 gcggatgaat ctcacctggg cacaggttgg cggtatttta aaatataccc gtccggcgtg
    22501 gtggcgtggc gaaacgcctg agacacatca ctatttaatg aaaaagccgg gttattatct
    22561 ttctgaagaa gcctatattg cccggttgcg taaagaactt aatttggcgc tttacagtcg
    22621 ttttccatta acttggatta tggaagctgc cgacgacatc tcctattgtg tggcagacct
    22681 tgaagatgcg gtagagaaaa gaatatttac cgttgagcag ctttatcatc atttgcacga
    22741 agcgtggggc cagcatgaga aaggttcgct cttttcgctg gtggttgaaa atgcctggga
    22801 aaaatcacgc tcaaatagtt taagccgcag tacggaagat cagtttttta tgtatttacg
    22861 ggtaaacacc ctaaataaac tggtacccta cgcggcacaa cgatttattg ataatctgcc
    22921 tgcgattttc gccggaacgt ttaatcatgc attattggaa gatgccagcg aatgcagcga
    22981 tcttcttaag ctatataaaa atgtcgctgt aaaacatgtg tttagccatc cagatgtcga
    23041 gcagcttgaa ttgcagggct atcgggtcat tagcggatta ttagagattt atcgtccttt
    23101 attaagcctg tcgttatcag actttactga actggtagaa aaagaacggg tgaaacgttt
    23161 ccctattgaa tcgcgcttat tccacaaact ctcgacgcgc catcggctgg cctatgtcga
    23221 ggctgtcagt aaattaccgt cagattctcc tgagtttccg ctatgggaat attattaccg
    23281 ttgccgcctg ctgcaggatt atatcagcgg tatgaccgac ctctatgcgt gggatgaata
    23341 ccgacgtctg atggccgtag aacaataacc aggcttttgt aaagacgaac aataaatttt
    23401 taccttttgc agaaacttta gttcggaact tcgcgctata aaacgaatct gaagaacaca
    23461 gcaattttgc gttatctgtt aatcgagact gaaatacatg aaaaaaacca cattagcact
    23521 gagtgcactg gctctgagtt taggtttggc gttatctccg ctctctgcaa cggcggctga
    23581 gacttcttca gcaacgacag cccagcagat gccaagcctt gcaccgatgc tcgaaaaggt
    23641 gatgccttca gtggtcagca ttaacgtaga aggtagcaca accgttaata cgccgcgtat
    23701 gccgcgtaat ttccagcagt tcttcggtga tgattctccg ttctgccagg aaggttcacc
    23761 gttccagagc tctccgttct gccagggggg ccagggcggt aatggcggcg gccagcaaca
    23821 gaaattcatg gcgctgggtt ccggcgtcat cattgatgcc gataaaggct atgtcgtcac
    23881 caacaaccac gttgttgata acgcgacggt gattaaagtc cagctgggcg atggtcgtaa
    23941 gttcgacgcg aagatggttg gcaaagatcc gcgctctgat atcgcgctga ttcagatcca
    24001 gaacccgaaa aacctgaccg caattaagat ggcggattct gatgcactgc gcgtgggtga
    24061 ttacaccgta gcgattggta acccgtttgg cctgggagag acggtaactt ccgggattgt
    24121 ctctgcgctg gggcgtagcg gcctgaatgc cgaaaactac gaaaacttca tccagaccga
    24181 tgcagcgatc aaccgtggta actccggtgg tgcgctggtt aacctgaacg gcgaactgat
    24241 cggtatcaac accgcgatcc tcgcaccgga cggcggcaac atcggtatcg gttttgctat
    24301 cccgagcaac atggtgaaaa acctgacctc gcagatggtg gaatacggcc aggtgaaacg
    24361 cggtgagctg ggtattatgg ggactgagct gaattccgaa ctggcgaaag cgatgaaagt
    24421 tgacgcccag cgcggtgctt tcgtaagcca ggttctgcct aattcctccg ctgcaaaagc
    24481 gggcattaaa gcgggtgatg tgatcacctc actgaacggt aagccaatca gcagctttgc
    24541 cgcactgcgt gctcaggtgg gtactatgcc ggtgggcagc aaactgaccc tgggcttgct
    24601 gcgcgacggt aagcaggtca acgtgaatct ggaactgcag cagagcagcc agaatcaggt
    24661 tgattccagc tccatcttca acggcattga aggcgctgag atgagcaaca aaggcaaaga
    24721 tcagggcgtg gtagtgaaca acgtgaaaac gggcactccg gctgcgcaga tcggcctgaa
    24781 gaaaggtgat gtgattattg gcgcgaacca gcaggcagtg aaaaacatcg ctgaactgcg
    24841 taaagttctt gacagcaaac cgtctgtact ggcactgaac attcagcgcg gcgacagctc
    24901 catctacctg ttaatgcagt aatctccctc aaccccttcc tgaaaacggg aaggggttct
    24961 ccttacaatc tgtgaacttc accacaactc catacatctt catcatcctt taggcatttg
    25021 cacaatgccg tacgttacgt acttccttat gctaagccgt gcataacgga ggacttatgg
    25081 ctggcatctt gataccaaaa tggcgcagga tatcgtggca cgtaccatgc gcatcatcga
    25141 taccaatatc aacgtaatgg atgcccgtgg gcgaattatc ggcagcggcg atcgtgagcg
    25201 tattggtgaa ttgcacgaag gtgcattgct ggtactttca cagggacgag tcgtcgatat
    25261 cgatgacgcg gtggcacgtc atctgcacgg tgtgcggcaa gggattaatc taccgttacg
    25321 gctggaaggt gaaattgtcg gcgtaattgg cctgacaggt gaaccagaga atctgcgtaa
    25381 atatggcgaa ctggtctgca tgacggctga aatgatgctg gaacagtcgc ggttgatgca
    25441 cttgttggct caggatagcc gtttgcggga ggaactggtg atgaacctga ttcaggcaga
    25501 ggagaatact cccgcactta ctgaatgggc gcaacggctg gggatcgatc tcaatcaacc
    25561 gcgagtggtg gctattgttg aggtcgacag cggtcagctt ggtgtggaca gcgcaatggc
    25621 ggagttacaa caactgcaaa acgcgctgac tacgcccgag cgtaataatc tggtggcgat
    25681 tgtctcgcta accgaaatgg tggtgttgaa accggcgttg aactcttttg ggcgctggga
    25741 tgcagaagat catcgtaagc gagttgaaca actgattacc cgcatgaaag agtacggcca
    25801 gctgcgtttt cgcgtttcac tgggcaacta ttttaccggt cctggcagta ttgcccgatc
    25861 ctatcgtacg gcgaaaacga cgatggtggt gggtaaacag cggatgccag aaagtcgctg
    25921 ctatttttat caggatctga tgttacctgt gttactcgac agtttgcgtg gcgactggca
    25981 ggccaacgaa ctggcgcgac cgctggcgcg gctgaaagcg atggacaata acggcttgct
    26041 gcgacgaacg ctggcggcgt ggtttcgtca caatgtgcaa ccgctggcaa cgtcaaaggc
    26101 gttgtttatt catcgtaata ccctggagta tcggcttaat cgtatatcgg aactgaccgg
    26161 gcttgatttg ggcaattttg atgacaggtt gctgctgtat gtggcgttac aactggatga
    26221 agagcggtag gttatgcgtt aaggttgagg cggcgctggc tcattcccct cagcccgaag
    26281 gggagagggg aatgcccgaa ttaaccgtca attcgggatt atttacgcgt tagtttttcc
    26341 agatcggctt caatctcgct gatcttattg gttacgactg actccaggtg acgtaagtcg
    26401 tcgaggatct tacgcttaag atcaacttcg ctgcggtcac gctggcagat ttgatcaagc
    26461 tcatcaatga tataccgtag attcgggctg atttcctgga cttctttata accctgaccc
    26521 acaccatcag cgacgaccgt tttacgctga cgcggatatt taaacttaac gctcttggcg
    26581 aaaaactcgc ctttgtcttt ctggaaatag attttcagaa tatcgttgtt ggcttcctgc
    26641 cggaggctgt aacgatcaat ttcttcagga ttggtaatac ccagactttt cagattgtcg
    26701 tacatagcgt tacctcaaaa tgagtcagta aattgtgctt attttagcat ttggcctggc
    26761 ccgcccggct cgactgtgat cgcagaaagc ctttttcttc cagacttcta tcatggcgta
    26821 aaaatcaaaa aattacctgc tttattgtgg tgataaaatt cacgttctac accagttcca
    26881 gcatcgttac ttccgggcgg cagttcagac gcaaaccata caaactgccc acgccacggg
    26941 ttgtgtagat gtgtcgttcg ccaaaggcat ttaatccggc gacgtaacgt ttatcttcga
    27001 caggggcaaa aggttcacca accaacggta cgcgcagttg cccgccgtga gtatggccgc
    27061 acagcatcag atcccagggt tcatcacgca tgacttcttt gctgtcggga ttatgcgcca
    27121 gcaccagtct tggcagattg gcttcgctgg cgggaggcgg tttgcattgt ccggcccata
    27181 agtcaccggt gcctaccagt tcgaattgcc tgttcggcgt ggcgatcacc gtagcctggt
    27241 taaacaacac cgtgatgccc gctgatttca acgtctcgcc aattaagtga tttttttctg
    27301 taccaacagg gcgatcgtgg ttgccgaagc aggcaaacgt cggcgcacat tcggcaaggg
    27361 gggagaggac atcactaaac gccgaaaaat tcagcggcat atcaaataat acgtaatcgc
    27421 cgcccagtaa tatcaaatcg ggcttttgtt ctatgccaag agcaatcgcg tcagaaatca
    27481 ggcttaaagg aacaaaacga gagtaatgga gatcggccag aaaaagaatt ttgaatggtg
    27541 ctgcgttgtc tttaaaaaag gcgaggcggt gacggattaa ttcaaaccag cctggctccc
    27601 agtaatgcat ataaccgaaa cctgagctcg tggctatcgt cgcggcagta gcctgcaaca
    27661 atcggcggcg tgaaatcatc gctcatccct gcaaaaaaaa tcgggcagcg tcgtgctgcc
    27721 cgtgtgcata cttttagtcg atggtacgca gcagttcgtt aatgccgact ttgccgcgag
    27781 ttttcgcgtc aactttctta acgataaccg cacagtagag gctgtatttg ccatcttttg
    27841 acggcagatt acctgaaaca accacagacc ccgccggaac gcgaccgtag tggatttcgc
    27901 cggtttcacg gtcgtaaata cgggtgctct gaccaatgta tacgcccatg gaaatgacgg
    27961 aaccttcttc gacaatcacc ccttcaacca cttcagagcg tgcgccaatg aagcagttat
    28021 cttcaatgat ggttgggtta gcctgcagcg gttccagcac gccgccgatg cccacgccac
    28081 cggaaaggtg gacgttttta ccaatctgcg cacaagaacc gacggtcgcc caggtatcaa
    28141 ccatggtgcc ttcatcaaca tatgcgccga tgttgacgta agacggcatc agcacggtgt
    28201 tacgggcaat aaacgcaccc tgacgtaccg ccgctggtgg cacaacgcgg aagccttctt
    28261 tctggaaacg tgcttcgtcg tagtcggcga atttcatcgg cactttgtcg aagtagcggc
    28321 tttctgcccc ttcgatcacc tgattatcat taatacggaa agagagcagc accgcttttt
    28381 tcaaccactg atgcgtcacc cactgaccgt caattttttc cgctacacgc agtgcaccgg
    28441 aatccagcag ggcgatcacc tgatttaccg cttcgcgggt aacggtgtct gcgttggctg
    28501 gcgtgatctc ggcacggcgt tcaaaagcgg tttcaataat gttctgtaac tgctgcattg
    28561 ttaaactctt ttcatatcag taaacacatc accctttatc gtttggattg agggcctctg
    28621 tcaaccgctg atgcacttcc tgctgcaact cgttattaag tgcacgccgg tcagcggtgg
    28681 caattatgaa taaatcttct actcgctcgc caatggttgt aattcgggca ccatgaagcg
    28741 aaattcccag atcggcaaaa attttcccga ctctcgccag cagtcctggc tggtcgagcg
    28801 cgataagctc aaggaacgat ttgcggtcgg tatgggtcgg caaaaacgtt acttcggttt
    28861 caacagtaaa atggcgtaat ttggcgggtt ggcgacgggg ctgcggtggc tgccagctac
    28921 tttgcgtcag tacttgctcc agaccaaacc gaataacctc atgacgatct gcggacagcg
    28981 ggctgccatc gggttccagc acgataaagg tatccatcgc cataccgtcg cgagtggtga
    29041 aaatttgtgc gtcgtgaaca cttaaattgc ggcggtctaa ttcggcacag acggcggcaa
    29101 acagataggg gcggtccggg ctccagataa aaatctcggt gcctccacgc gtagcctgcg
    29161 ggctaagcaa taccagcggt ttgcttaaat catgctgtaa taaatggcgg gcatgccagg
    29221 ccagttgatt tgggctatgg cggacaaaat agttagcacg acagcgtgac caaatttggt
    29281 gcagcgcctc ttcgtcgatg ttatccatgc gcagtagtgc cagtgcctgg agttggtgat
    29341 gacgaacccg ttcgcgcata tccggcgtgt tttgcattcc gcgtcgtagc tgcttttcgg
    29401 tggcaaaata gagttcgcgc aacagacttt gcttccagct attccacagc gtttcgttgg
    29461 tggcgcaaat gtcagccacc gtcaggcata ccagataacg aagtcggttt tctgtttgca
    29521 cttcttcggc aaactgcttg atgacttccg ggtcctgaat atcgcggcgt tgggcggtca
    29581 ccgacatcaa caggtgctgg cgaaccagcc aggcaaccag ctgtgtttcg cgcgagttaa
    29641 gcccgtggag ttcggcaaaa tgcactacat cctgagcacc gagaatggag tggtcgccgc
    29701 cgcgtccttt ggcgatatcg tgaaacagcg cggcgatgaa aatcagctca gttgacggca
    29761 ggcgcggcca gacgtccaca cacaacggat ggcgctggcg cgtttcttca ctggcaaaac
    29821 tctccagttt cagcatcacg cggatagtat gttcatccac cgtgtaggcg tggaacagat
    29881 caaactgcat ctgcccgacg atatgcgacc attgcggcat atacgcgccg agcacgctat
    29941 ggcgatgcat tggcaatagc ccgcgccgca ccgctccggg gtgacgcaga atgctcaaaa
    30001 acagttttcg tgcttccgga atattacaca gcggttgttg cagatggcga cgggcatggc
    30061 gtaactggcg cagcgtggtg gagtaaatgc cggtgatcgc actgttgcgc accatggtgt
    30121 agaacatacg caagatggct tccggctggc gcataaatag tgtttcatca cgcaggtcga
    30181 ttagcgtacc gcgtagctga aactcatcgt cgagtggacg tggtttttcg tcggcgggaa
    30241 gggcgaggat ggcttcatcg aacagttgca gcagcatctg gttgagttca ctgacgcggc
    30301 gtgtaacgcg gaagtaatcc ttcatcatcc gctcgaccgg ttcgttgcct tcaccactgt
    30361 aattcagacg ctgagcgacg ctaagttggc gatcgaataa cagacgatta tcgtaacggc
    30421 tgacgaccag atgcagggca aagcgaatac gccacagtat atgcagacat tcgtttaatt
    30481 ccgcccgctc cgctgaggtt aagaagccaa acccaaccat ttcatccagc gatgttgcgc
    30541 caaaatgacg gcgggccacc cattgcagag tgtggatatc gcgcaagccg ccagggctac
    30601 ttttgatgtc tggttcaaga ttgtagctgg tgccgtggta acgctgatgg cgctggttct
    30661 gttcttcaac tttcgccgcg tagaacttgt cggaaggcca gaatccttcg ctaaaaatat
    30721 gtttttgcag ttcgaggaag agcgcaacat cgccaattaa taagcgggat tcgattaaat
    30781 tggtggcgac ggttaaatcc gataaccctt ccagcatgca ctcttcaagc gtgcgcacac
    30841 tatgaccgac ttccagcttt acatcccaga gcagcgttaa cagctcgccc actttttgcg
    30901 cctgatcgtc cgggagcttt ttacggctta aaatcagtaa atcgatgtca gaaagtggat
    30961 gcagctcgcc acgaccgtag ccaccgacgg cgaccaatgc caggtcggca atctggctga
    31021 atcccgcttc aatccataat cgttgcagga gctggtcgat aaactcggtg cgcgcctcaa
    31081 tcaactgttc tgcagagatc ccattgtcaa aggcatcacc cagccaacgc tggaaagtat
    31141 cgatatgggc ttttatccca ccgacggtta attcatcacg gggccagacg catggatttt
    31201 gcggttgacc gggcagggtg gggagagcgg tgtttgcgta ctgttctgga agggtattca
    31261 ttgtgcgcca cccataagat taaattatcg cattaaaaaa gccggcattc gccggcttca
    31321 tcttattcgt cgtgcgagat tatcgccggg atggtgtcat ccttgcgtag cgtcagaatt
    31381 tcgcagccgt tatcagtcac cacaatagta tgctcatatt gtgcagacaa gctgcgatct
    31441 ttggttttta ccgtccagcc atctttcatg gtgcggatct cttttttacc cgcgttgacc
    31501 attggctcga tggtgaacgt catcccaggt ttcagtacga cgttggtttc acgggagtca
    31561 tagtgcagca cctgcggttc ttcatggaag ccgcgaccaa taccgtgtcc gcaatattca
    31621 cgaacgacgg agaagccttc tgcttcgaca aatttctgaa tcgccgcacc gatttcgcgc
    31681 agattaatgc ctggtttgac catgcgcagc gccaggtaca ggctttcttg cgtgatgcgg
    31741 cacagacgtt cgcccatgat ggtcggctta ccgacgataa acattttcga ggtatcgccg
    31801 tggaaaccat ctttgattac ggtgacatca atgttaacga tatcgccatc tttcagcagc
    31861 ttcgcatcgt ccgggatacc gtggcacacc acttcattaa tagagatgca aacggatttc
    31921 ggatagccgt gatagccgag gcaggcagaa accgtgtgtt gttcattaac aatgtaatca
    31981 ttacagatgc gatccagctc gccggtgctg acgcccggtt taacatacgg ttcgatcatc
    32041 tccagcactt cggcagccag tcggccagcg acgcgcattt tttcgatatc ttctggggtc
    32101 ttgattgaga tagccattaa ttctgtccat cagcgtcggt gataccgaca atatatatgt
    32161 aagtgccgtc aatggtatca cacccgggca aattgagaat cattctgaat ttcgccaaac
    32221 gtgccactga acgttttcta taatagaaaa ttcaacgtct gatgctgtac acagcgccaa
    32281 caattattgg tgtccatgac gtatttgtgg tataaagcgc gccggacttc cgatccattt
    32341 cgtatacaca gactggacgg aagcgacaat ctcactttgt gtaacaacac acacgtatcg
    32401 gcacatattc cggggtgccc tttggggtcg gtaatatggg atacgtggag gcataacccc
    32461 aacttttata tagaggtttt aatcatggca actgtttcca tgcgcgacat gctcaaggct
    32521 ggtgttcact tcggtcacca gacccgttac tggaacccga aaatgaagcc gttcatcttc
    32581 ggtgcgcgta acaaagttca catcatcaac cttgagaaaa ctgtaccgat gttcaacgaa
    32641 gctctggctg aactgaacaa gattgcttct cgcaaaggta aaatcctttt cgttggtact
    32701 aaacgcgctg caagcgaagc ggtgaaagac gctgctctga gctgcgacca gttcttcgtg
    32761 aaccatcgct ggctgggcgg tatgctgact aactggaaaa ccgttcgtca gtccatcaaa
    32821 cgtctgaaag acctggaaac tcagtctcag gacggtactt tcgaaaagct gaccaagaaa
    32881 gaagcgctga tgcgcactcg tgagctggag aaactggaaa acagcctggg cggtatcaaa
    32941 gacatgggcg gtctgccgga cgctctgttt gtaatcgatg ctgaccacga acacattgct
    33001 atcaaagaag caaacaacct gggtattccg gtatttgcta tcgttgatac caactctgat
    33061 ccggacggtg ttgacttcgt tatcccgggt aacgacgacg caatccgtgc tgtgaccctg
    33121 tacctgggcg ctgttgctgc aaccgtacgt gaaggccgtt ctcaggatct ggcttcccag
    33181 gcggaagaaa gcttcgtaga agctgagtaa taaggcttga taactccccc aaaatagttc
    33241 gagttgcaga aaggcggcaa gctcgagaat tcccgggagc ttacatcagt aagtgaccgg
    33301 gatgagcgag cgaagacaac gcatctgcgg cgcgaaatat gaagggggag agcccttata
    33361 gaccaggtag tacacgtttg gttagggggc ctgcatatgg cccccttttt cacttttata
    33421 tctgtgcggt ttaatgccgg gcagatcaca tctccgagga ttttagaatg gctgaaatta
    33481 ccgcatccct ggtaaaagag ctgcgtgagc gtactggcgc aggcatgatg gattgcaaaa
    33541 aagcactgac tgaagctaac ggcgacatcg agctggcaat cgaaaacatg cgtaagtccg
    33601 gtgctattaa agcagcgaaa aaagcaggca acgttgctgc tgacggcgtg atcaaaacca
    33661 aaatcgacgg caactacggc atcattctgg aagttaactg ccagactgac ttcgttgcaa
    33721 aagacgctgg tttccaggcg ttcgcagaca aagttctgga cgcagctgtt gctggcaaaa
    33781 tcactgacgt tgaagttctg aaagcacagt tcgaagaaga acgtgttgcg ctggtagcga
    33841 aaattggtga gaacatcaac attcgccgcg ttgctgcgct ggaaggcgac gttctgggtt
    33901 cttatcagca cggtgcgcgt atcggcgttc tggttgctgc taaaggcgct gacgaagagc
    33961 tggttaaaca catcgctatg cacgttgctg caagcaagcc agaattcatc aaaccagaag
    34021 acgtatccgc tgaagtggta gaaaaagaat accaggtaca gctggatatc gcgatgcagt
    34081 ctggtaagcc gaaagaaatc gcagagaaaa tggttgaagg ccgcatgaag aaattcaccg
    34141 gcgaagtttc tctgaccggt cagccgttcg ttatggaacc aagcaaaact gttggtcagc
    34201 tgctgaaaga gcataacgct gaagtgactg gcttcatccg cttcgaagtg ggtgaaggca
    34261 tcgagaaagt tgagactgac tttgcagcag aagttgctgc gatgtccaag cagtcttaat
    34321 tatcaaaaag gagccgcctg agggcggctt ctttttgtgc ccatcttgta aattcagcta
    34381 acccttgtgg ggctgcgctg aaaagcgacg tacaatgtcg ctagtattaa ttcatttcaa
    34441 tcgttgacag tctcaggaaa gaaacatggc taccaatgca aaacccgtct ataaacgcat
    34501 tctgcttaag ttgagtggcg aagctctgca gggcactgaa ggcttcggta ttgatgcaag
    34561 catactggat cgtatggctc aggaaatcaa agaactggtt gaactgggta ttcaggttgg
    34621 tgtggtgatt ggtgggggta acctgttccg tggcgctggt ctggcgaaag cgggtatgaa
    34681 ccgcgttgtg ggcgaccaca tggggatgct ggcgaccgta atgaacggcc tggcaatgcg
    34741 tgatgcactg caccgcgcct atgtgaacgc tcgtctgatg tccgctattc cattgaatgg
    34801 cgtgtgcgac agctacagct gggcagaagc tatcagcctg ttgcgcaaca accgtgtggt
    34861 gatcctctct gccggtacag gtaacccgtt ctttaccacc gactcagcag cttgcctgcg
    34921 tggtatcgaa attgaagccg atgtggtgct gaaagcaacc aaagttgacg gcgtgtttac
    34981 cgctgatccg gcgaaagatc caaccgcaac catgtacgag caactgactt acagcgaagt
    35041 gctggaaaaa gagctgaaag tcatggacct agcggccttc acgctggctc gtgaccataa
    35101 attaccgatt cgtgttttca atatgaacaa accgggtgcg ctgcgccgtg tggtaatggg
    35161 tgaaaaagaa gggactttaa tcacggaata attcccgtga tggataaata agggtaagat
    35221 tccgcgtaag tatcgcgggg gcgttagtct ggttataagg cgttattgtt gcaggcagtt
    35281 tggtcacggc cagcgcgcag caaccggagc gtacaaaagt acgtgaggat ggcgagcact
    35341 gctcggggcc aaaatggcaa ataaaatagc ctaataatcc agacgattac ccgtaatatg
    35401 tttaatcagg gctatactta gcacacttcc actgtgtgtg actgtctggt ctgactgaga
    35461 caagttttca aggattcgta acgtgattag cgatatcaga aaagatgctg aagtacgcat
    35521 ggacaaatgc gtagaagcgt tcaaaaccca aatcagcaaa atacgcacgg gtcgtgcttc
    35581 tcccagcctg ctggatggca ttgtcgtgga atattacggc acgccgacgc cgctgcgtca
    35641 gctggcaagc gtaacggtag aagattcccg tacactgaaa atcaacgtgt ttgatcgttc
    35701 aatgtctccg gccgttgaaa aagcgattat ggcgtccgat cttggcctga acccgaactc
    35761 tgcgggtagc gacatccgtg ttccgctgcc gccgctgacg gaagaacgtc gtaaagatct
    35821 gaccaaaatc gttcgtggtg aagcagaaca agcgcgtgtt gcagtacgta acgtgcgtcg
    35881 tgacgcgaac gacaaagtga aagcactgtt gaaagataaa gagatcagcg aagacgacga
    35941 tcgccgttct caggacgatg tacagaaact gactgatgct gcaatcaaga aaattgaagc
    36001 ggcgctggca gacaaagaag cagaactgat gcagttctga tttcttgaac gacaaaaacg
    36061 ccgctcagta gatccttgcg gatcggctgg cggcgttttg ctttttattc tgtctcaact
    36121 ctggatgttt catgaagcaa ctcaccattc tgggctcgac cggctcgatt ggttgcagca
    36181 cgctggacgt ggtgcgccat aatcccgaac acttccgcgt agttgcgctg gtggcaggca
    36241 aaaatgtcac tcgcatggta gaacagtgcc tggaattctc tccccgctat gccgtaatgg
    36301 acgatgaagc gagtgcgaaa cttcttaaaa cgatgctaca gcaacagggt agccgcaccg
    36361 aagtcttaag tgggcaacaa gccgcttgcg atatggcagc gcttgaggat gttgatcagg
    36421 tgatggcagc cattgttggc gctgctgggc tgttacctac gcttgctgcg atccgcgcgg
    36481 gtaaaaccat tttgctggcc aataaagaat cactggttac ctgcggacgt ctgtttatgg
    36541 acgccgtaaa gcagagcaaa gcgcaattgt taccggtcga tagcgaacat aacgccattt
    36601 ttcagagttt accgcaacct atccagcata atctgggata cgctgacctt gagcaaaatg
    36661 gcgtggtgtc cattttactt accgggtctg gtggcccttt ccgtgagacg ccattgcgcg
    36721 atttggcaac aatgacgccg gatcaagcct gccgtcatcc gaactggtcg atggggcgta
    36781 aaatttctgt cgattcggct accatgatga acaaaggtct ggaatacatt gaagcgcgtt
    36841 ggctgtttaa cgccagcgcc agccagatgg aagtgctgat tcacccgcag tcagtgattc
    36901 actcaatggt gcgctatcag gacggcagtg ttctggcgca gctgggggaa ccggatatgc
    36961 gtacgccaat tgcccacacc atggcatggc cgaatcgcgt gaactctggc gtgaagccgc
    37021 tcgatttttg caaactaagt gcgttgacat ttgccgcacc ggattatgat cgttatccat
    37081 gcctgaaact ggcgatggag gcgttcgaac aaggtcaggc agcgacgaca gcattgaatg
    37141 ccgcaaacga aatcaccgtt gctgcttttc ttgcgcaaca aatccgcttt acggatatcg
    37201 ctgcgttgaa tttatccgta ctggaaaaaa tggatatgcg cgaaccacaa tgtgtggacg
    37261 atgtgttatc tgttgatgcg aacgcgcgtg aagtcgccag aaaagaggtg atgcgtctcg
    37321 caagctgagg ataatccggc tacagagagt cgcgctattt gttagcgtag ggcttcagtg
    37381 atatagtctg cgccatctga tcgtaagtag ttggctttat aaggtcaggt atgccgtggt
    37441 tttacacggc ttttttttgt ataggcttca gtattcctga gtaccgtaaa ccctgtcagg
    37501 gaataaaaaa cgcgtgatgt tgtctgctac tcaaccactt agcgaaaaat tgccagcgca
    37561 tggctgccgt catgttgcga tcattatgga cggcaatggt cgctgggcaa aaaagcaagg
    37621 gaagattcgt gcctttgggc ataaagccgg ggcaaaatcc gtccgccggg ctgtctcttt
    37681 tgcggccaac aacggtattg aggcgttaac gctgtatgcc tttagtagtg aaaactggaa
    37741 ccgaccagcg caggaagtca gtgcgttaat ggaactgttt gtgtgggcgc tcgatagcga
    37801 agtaaaaagt ctgcaccgac ataacgtgcg tctgcgtatt attggcgata ccagtcgctt
    37861 taactcgcgt ttgcaagaac gtattcgtaa atctgaagcg ctaacagccg ggaatagcgg
    37921 tctgacgctg aatattgcgg cgaactacgg tggacgttgg gatatagtcc agggagtcag
    37981 gcaactggct gaaaaggtgc agcaaggaaa cctgcaacca gatcagatag atgaagagat
    38041 gctaaaccag catgtctgta tgcatgaact ggcccctgta gatttagtaa ttaggactgg
    38101 gggggagcat cgcattagta actttttgct ttggcaaatt gcctatgccg aactttactt
    38161 tacagatgtt ctctggcccg atttcgatga acaagacttt gaaggggcgt taaatgcctt
    38221 tgctaatcga gagcgtcgtt tcggcggcac cgagcccggt gatgaaacag cctgatgggg
    38281 gtcgcttttg ctgaagtatc gcctgatatc tgcttttgtg ttaatacccg tcgtcatcgc
    38341 ggcgttgttt ctgttgccgc cggtggggtt cgccatcgta acgctggtgg tctgcatgct
    38401 ggcagcgtgg gaatggggac agcttagcgg ttttaccact cgttcgcagc gagtatggtt
    38461 ggcggtgtta tgcgggttat tgttggcgct gatgcttttt ctgttgccgg aatatcaccg
    38521 aaatattcat caaccgctgg ttgaaatctc actttgggct tcgttgggtt ggtggattgt
    38581 cgcgctattg ctggtgctgt tttacccagg ttccgcagca atctggcgta actctaaaac
    38641 attgcgcctt atttttggcg tgctaaccat tgttcccttc ttctggggca tgctggcgtt
    38701 acgggcctgg cactacgacg agaatcatta cagtggcgca atatggctgc tctatgtcat
    38761 gatcctggta tggggcgctg actctggcgc atatatgttt ggcaaattgt ttggtaaaca
    38821 taagctggca ccgaaggttt ctccgggtaa aacctggcaa ggctttatag gtggactagc
    38881 gaccgcagcg gtaatctcat ggggttatgg catgtgggcg aatctcgacg tcgctcccgt
    38941 caccttactc atttgctcta ttgtcgcagc gttagcctca gtgctcggcg atctgaccga
    39001 gagtatgttt aagcgtgaag caggaattaa ggacagcggt catttaattc caggacacgg
    39061 tggtatttta gatcgtattg atagcctgac ggctgcggta ccggtctttg cttgcttgtt
    39121 gttactggta ttcaggacgc tttaacggaa ggtaatatgc tgagttttct ctgggatttg
    39181 gcttcgttca tcgttgcact gggtgtactt atcaccgtgc atgaatttgg tcatttctgg
    39241 gttgcccggc gttgtggtgt tcgcgttgag cgtttctcaa tagggtttgg taaggcgctc
    39301 tggcggcgaa ctgataagct cggcaccgaa tatgttatcg ccctgatccc gctgggcggt
    39361 tatgtcaaaa tgctggatga gcgcgcagaa ccggtcgttc cggaactccg ccaccatgcc
    39421 ttcaataata aatctgtcgg ccaacgagcg gcgattattg ccgcaggtcc ggttgcaaac
    39481 ttcatttttg ctatctttgc ctactggctg gtttttatta ttggtgtgcc tggcgtacgt
    39541 ccggtggttg gtgaaatagc agccaattcg atagctgcgg aagcacaaat tgcaccaggt
    39601 acggaactaa aagccgtaga tggtatcgaa acgcctgatt gggatgccgt gcgtttgcag
    39661 ttggtcgata aaattggcga tgaaagcacc accattacag tagcgccatt tggcagcgac
    39721 caacggcggg atgtaaagct cgatttacgt cactgggcgt ttgagcctga taaagaagat
    39781 ccggtatctt cgctggggat tcgtcctcgt gggccgcaaa ttgaacctgt actggaaaat
    39841 gtgcaaccaa actcggcggc aagcaaggca ggtttgcaag caggcgacag gatcgttaaa
    39901 gtcgatggtc agcccttaac gcagtgggtg acctttgtga tgcttgtccg ggataacccg
    39961 ggtaaatcct tagcgttaga aatcgaaagg cagggaagtc ccttgtcttt gacattaatc
    40021 ccggagagta aaccgggtaa tggtaaagcg attggttttg tcggtattga gccgaaagtc
    40081 attcctttgc cagatgagta taaagttgta cgccagtatg ggccgttcaa cgccatcgtc
    40141 gaagccacgg acaaaacgtg gcagctgatg aagctgacgg tcagtatgct gggaaaattg
    40201 atcaccggtg atgtgaaact gaacaacctc agtgggccga tctctatcgc caagggggct
    40261 gggatgacag cggaactcgg ggtagtttat tacctgccgt ttcttgcgct tattagcgtg
    40321 aacttaggga taattaacct gtttccgttg cccgtacttg acggggggca tctgctgttc
    40381 cttgcgatcg aaaagatcaa gggcggaccg gtatccgagc gggttcaaga cttttgttat
    40441 cgcattggct cgattctgct ggtgctgtta atggggcttg cacttttcaa tgatttctct
    40501 cggttatgag agttagttag gaagaacgca taataacgat ggcgatgaaa aagttgctca
    40561 tagcgtcgct gctgtttagc agcgccaccg tatacggtgc tgaagggttc gtagtgaaag
    40621 atattcattt cgaaggcctt cagcgtgtcg ccgttggtgc ggccctcctc agtatgccgg
    40681 tgcgcacagg cgacacggtt aatgatgaag atatcagtaa taccattcgc gctctgtttg
    40741 ctaccggcaa ctttgaggat gttcgcgtcc ttcgtgatgg tgataccctt ctggttcagg
    40801 taaaagaacg tccgaccatt gccagcatta ctttctccgg taacaaatcg gtgaaagatg
    40861 acatgctgaa gcaaaacctc gaggcttctg gtgtgcgtgt gggcgaatcc ctcgatcgca
    40921 ccaccattgc cgatatcgag aaaggtctgg aagacttcta ctacagcgtc ggtaaatata
    40981 gcgccagcgt aaaagctgtc gtgaccccgc tgccgcgcaa ccgtgttgac ctaaaactgg
    41041 tgttccagga aggtgtgtca gctgaaatcc agcaaattaa cattgttggt aaccatgctt
    41101 tcaccaccga cgaactgatc tctcatttcc aactgcgtga cgaagtgccg tggtggaacg
    41161 tggtaggcga tcgtaaatac cagaaacaga aactggcggg cgaccttgaa accctgcgca
    41221 gctactatct ggatcgcggt tatgcccgtt tcaacatcga ctctacccag gtcagtctga
    41281 cgccagataa aaaaggtatt tacgtcacgg tgaacattac cgaaggcgat cagtacaagc
    41341 tttctggcgt tgaagtgagc ggcaaccttg ccgggcactc cgctgaaatt gagcagctga
    41401 ctaagatcga gccgggcgag ctgtataacg gcaccaaagt gaccaagatg gaagatgaca
    41461 tcaaaaagct tctcggtcgc tatggttatg cctatccgcg cgtacagtcg atgcctgaaa
    41521 ttaacgatgc cgacaaaacc gttaaattac gcgtgaacgt tgatgcgggt aaccgtttct
    41581 acgtgcgtaa gatccgtttt gaaggtaacg atacctcgaa agatgccgtc ctgcgtcgcg
    41641 aaatgcgtca gatggaaggt gcatggctgg gaagcgatct ggtcgatcag ggtaaggagc
    41701 gtctgaatcg tctgggcttc tttgaaactg tcgataccga tacccaacgt gttccgggta
    41761 gcccggacca ggtagatgtc gtctacaagg taaaagagcg caacaccggt agcttcaact
    41821 tcgggattgg ttacggtact gaaagtggcg tgagcttcca ggctggtgtg cagcaggata
    41881 actggttagg tacaggttat gctgttggta tcaacgggac caaaaacgat taccagacct
    41941 atgctgaact gtcggtaacc aacccgtact tcactgtaga tggtgtaagc ctcggtggtc
    42001 gtctcttcta taatgacttc caggcagatg acgccgacct gtccgactat accaacaaga
    42061 gttatggtac agacgtgacg ttgggcttcc cgatcaacga atataactcg ctgcgtgcag
    42121 gtctgggtta tgtacataac tccctgtcca acatgcagcc tcaggttgcg atgtggcgtt
    42181 atctgtactc tatgggtgaa catccgagca cctctgatca ggataatagc ttcaaaacgg
    42241 acgacttcac gttcaactat ggttggacct ataacaagct tgaccgtggt tacttcccga
    42301 cagatggttc acgtgtcaac ctgaccggta aagtgaccat tcctggatcg gataacgaat
    42361 actacaaagt gacgttagac acggcgactt atgtgccgat cgatgacgat cacaaatggg
    42421 ttgttctggg gcgtacccgc tggggttatg gtgatggttt aggcggcaaa gagatgccgt
    42481 tctacgagaa cttctatgcc ggtggttcca gcaccgtgcg tggcttccag tccaatacaa
    42541 ttggtccgaa agcagtttac ttcccgcatc aggccagtaa ttatgatccg gactatgatt
    42601 acgaatgtgc gactcaggac ggcgcgaaag acctgtgtaa atcggatgat gctgtaggcg
    42661 gtaacgccat ggcggttgcc agcctcgagt tcatcacccc gacgccgttt attagcgata
    42721 agtatgctaa ctcggttcgt acttccttct tctgggatat gggtaccgtt tgggatacaa
    42781 actgggattc cagccaatat tctggatatc cggactatag tgatccaagc aatatccgta
    42841 tgtctgcggg tatcgcatta caatggatgt ccccattggg gccgttggtg ttctcctacg
    42901 cccagccgtt caaaaagtac gatggagaca aggcagaaca gttccagttt aacatcggta
    42961 aaacctggta agtgttctcc acaaaggaat gtagtggtag tgtagcgatg actttaggcg
    43021 atcaatataa gatcgccggg ccacgcaaag aactgcaccc tccggtgcaa atgggatggt
    43081 aaggagttta ttgtgaaaaa gtggttatta gcggcaggtc tcggtttagc actggcaact
    43141 tctgctcagg cggctgacaa aattgcaatc gtcaacatgg gcagcctgtt ccagcaggta
    43201 gcgcagaaaa ccggtgtttc taacacgctg gaaaatgagt tcaaaggccg tgccagcgaa
    43261 ctgcagcgta tggaaaccga tctgcaggct aaaatgaaaa agctgcagtc catgaaagcg
    43321 ggcagcgatc gcactaagct ggaaaaagac gtgatggctc agcgccagac ttttgctcag
    43381 aaagcgcagg cttttgagca ggatcgcgca cgtcgttcca acgaagaacg cggcaaactg
    43441 gttactcgta tccagactgc tgtgaaatcc gttgccaaca gccaggatat cgatctggtt
    43501 gttgatgcaa acgccgttgc ttacaacagc agcgatgtaa aagacatcac tgccgacgta
    43561 ctgaaacagg ttaaataagt aatgccttca attcgactgg ctgatttagc gcagcagttg
    43621 gatgcagaac tacacggtga tggcgatatc gtcatcaccg gcgttgcgtc catgcaatct
    43681 gcacaaacag gtcacattac gttcatggtt aacccaaaat accgtgagca tttaggcttg
    43741 tgccaggcgt ccgcggttgt catgacccag gacgatcttc ctttcgcgaa aagtgccgcg
    43801 ctggtagtga agaatcccta cctgacttac gcgcgcatgg cgcaaatttt agataccacg
    43861 ccgcagcccg cgcagaacat tgcacccagt gcggtgatcg acgcgacggc gaagctgggt
    43921 aacaacgtat cgattggcgc taacgcggtg attgagtccg gcgttgaact gggcgataac
    43981 gtgattatcg gtgccggttg cttcgtaggt aaaaacagca aaatcggtgc aggttcgcgt
    44041 ctctgggcga acgtaaccat ttaccatgag atccagatcg gtcagaattg cctgatccag
    44101 tccggaacag tggtaggcgc agacggcttt ggttatgcca acgatcgtgg taactgggtg
    44161 aagatcccac agattggtcg cgtaattatt ggcgatcgcg tggagatcgg tgcctgcaca
    44221 accatcgatc gcggcgcgct ggatgacact attattggca atggcgtgat cattgataac
    44281 cagtgccaga ttgcacataa cgtcgtgatt ggcgacaata cggcggttgc cggtggcgtc
    44341 attatggcgg gcagcctgaa aattggtcgt tactgcatga tcggcggagc cagcgtaatc
    44401 aacgggcata tggaaatatg cgacaaagtg acggttacgg gcatgggtat ggtgatgcgt
    44461 cccatcactg aaccaggcgt ctattcctca ggcattccgc tgcaacccaa caaagtctgg
    44521 cgcaaaaccg ctgcactggt gatgaacatt gatgacatga gcaagcgtct gaaatcgctt
    44581 gagcgcaagg ttaatcaaca agactaacgt tccatctttt gttcgccaaa ctttacggcc
    44641 tgtctcattc ttacgattgc ggcaggccgt gttattattg tcgtttctta tattttgaca
    44701 ggaagagtat cttgactact aacactcata ctctgcagat tgaagagatt ttagaacttc
    44761 tgccgcaccg tttcccgttc ttactggtgg atcgcgtgct ggattttgaa gaaggtcgtt
    44821 ttctgcgcgc agtaaaaaat gtctctgtca atgagccatt cttccagggc catttccctg
    44881 gaaaaccgat tttcccgggt gtgctgattc tggaagcaat ggcacaggca acaggtattc
    44941 tggcgtttaa aagcgtagga aaactggaac cgggtgagct gtactacttc gctggtattg
    45001 acgaagcgcg cttcaagcgc ccggtcgtgc ctggcgatca aatgatcatg gaagtcactt
    45061 tcgaaaaaac gcgccgcggc ctgacccgtt ttaaaggggt tgctctggtc gatggtaaag
    45121 tagtttgcga agcaacgatg atgtgtgctc gtagccggga ggcctgatac gtgattgata
    45181 aatccgcctt tgtgcatcca accgccattg tggaagaggg cgcgtcaatt ggcgcgaacg
    45241 cacacattgg tcctttttgt atcgttggac cccatgtcga aattggtgag ggtaccgtac
    45301 tgaaatctca cgttgtcgtg aatggtcata ctaaaattgg ccgcgataat gagatttatc
    45361 agttcgcctc catcggcgaa gttaaccagg atctgaaata tgctggcgaa ccgacccgtg
    45421 tggaaatcgg cgatcgtaac cgcattcgcg aaagcgtcac cattcatcgt ggcacagtcc
    45481 agggcggtgg attgacgaag gtgggcagcg acaacttact gatgatcaac gcgcacattg
    45541 cgcacgattg tacggtaggt aaccgctgta ttctcgccaa caacgcaacg ctggcgggtc
    45601 acgtatcggt tgacgacttc gcgatcatcg gcggcatgac cgcagtccat cagttctgca
    45661 tcattggtgc gcacgtgatg gttggcggct gctccggtgt ggcgcaggac gtccctcctt
    45721 atgtcattgc gcagggtaac cacgcaacgc cgttcggtgt caatatcgaa gggctgaagc
    45781 gccgcggatt cagccgtgag gcgattaccg ctatccgcaa tgcgtataag ctgatttatc
    45841 gtagcggtaa aacgctcgat gaagtgaaac cggaaattgc tgaactggcg gaaacatatc
    45901 cggaagtgaa agcctttacc gatttctttg cacgctcaac gcgcggtctg attcgttaat
    45961 gactgaacag cgtccattaa cgattgccct ggtcgccgga gaaacctccg gcgatatcct
    46021 gggggccggt ttaatccgcg ctctgaaaga acgtgtgccc aacgcccgct ttgttggtgt
    46081 tgccgggcca cgaatgcagg ctgaaggctg cgaagcctgg tacgaaatgg aagaactggc
    46141 ggtgatgggc attgttgaag tgctcggtcg tctgcgtcgc ttactgcata ttcgtgccga
    46201 tctgacaaag cgttttggcg aactgaagcc agatattttt gttggtattg atgcgcctga
    46261 cttcaatatt actcttgaag gtaacctcaa aaagcagggt atcaaaacca ttcattacgt
    46321 cagtccatcc gtctgggcgt ggcgacagaa acgcgttttc aaaataggca gagccaccga
    46381 tctggtgctc gcatttctgc ctttcgaaaa agcgttttat gacaaataca atgtaccgtg
    46441 ccgctttatc ggtcatacca tggctgatgc catgccgtta gatccagata aaaatgccgc
    46501 ccgtgatgtg ctggggatcc ctcacgatgc ccactgcctg gcgttgctac cagggagccg
    46561 tggtgcagaa gttgaaatgc ttagtgccga tttcctgaaa acggcccagc ttttgcgcca
    46621 gacatatccg gatctcgaaa tcgtggtgcc actggtgaat gccaaacgcc gcgagcagtt
    46681 tgaacgcatc aaagctgaag tcgcgccaga cctttcagtt catttgctgg atgggatggg
    46741 ccgtgaggcg atggtcgcca gcgatgcggc gctactggcg tcgggtacgg cagccctgga
    46801 gtgtatgctg gcgaaatgcc cgatggtggt gggatatcgc atgaagcctt ttaccttctg
    46861 gttggcgaag cggctggtga aaactgatta tgtctcgctg ccaaatctgc tggcgggtag
    46921 agagttagtc aaagaattat tgcaggaaga gtgtgagccg caaaaactgg ctgcggcgct
    46981 gttaccgctg ttggcgaacg ggaaaaccag ccacgcgatg cacgatacct tccgtgaact
    47041 gcatcagcag atccgctgca atgccgatga gcaggcggca caagccgttc tggagttagc
    47101 acaatgatcg aatttgttta tccgcacacg cagctggttg cgggtgtgga tgaagtcgga
    47161 cgcgggccgt tagttggcgc ggtcgtcacc gctgcggtga tccttgaccc ggcgcgcccg
    47221 attgccgggc tgaatgattc caaaaagctg agcgaaaaac gccgtctggc gctctatgaa
    47281 gagatcaaag agaaagcgtt gagctggagt ctgggccgcg cggaacccca cgaaatcgac
    47341 gagctgaaca ttcttcatgc gaccatgctg gcgatgcagc gtgccgtcgc tgggctgcat
    47401 attgcgccgg aatatgtgtt gattgatggt aaccgctgcc cgaaattacc gatgcctgcg
    47461 atggctgtgg tgaaaggcga tagccgcgta ccggaaatca gtgccgcgtc tatcctggcg
    47521 aaagtgacgc gtgacgccga aatggcggcg ctggatattg ttttcccgca atatggtttt
    47581 gcccaacaca aagggtaccc aaccgctttt catctggaaa aactggctga acacggcgcg
    47641 accgaacacc atcggcgcag ctttgggcct gtcaaacgcg cactgggact tgcgtcctga
    47701 ttcttgtgtc gagattaagt aaaccggaat ctgaagatgt ctgaaccacg tttcgtacac
    47761 ctgcgggtgc acagcgacta ctcgatgatc gatggcctgg ccaaaaccgc accgttggta
    47821 aaaaaggcgg cggcgttggg tatgccagca ctggcgatca ccgatttcac caacctttgt
    47881 ggtctggtga agttctacgg agcgggacat ggcgcaggga ttaagcctat cgtcggggca
    47941 gattttaacg tccagtgcga cctgctgggt gatgagttaa cccacctgac ggtactggcg
    48001 gcgaacaata ccggctatca gaatctgacg ttgctgatct caaaagcgta tcagcgcggg
    48061 tacggtgccg ccgggccgat catcgatcgc gactggctta tcgaattaaa cgaagggttg
    48121 atccttcttt ccggcggacg catgggcgac gtcggacgca gtcttttgcg tggtaacaga
    48181 gcgctggtag atgagtgtgt cgcgttttat gaagaacact tcccggatcg ctattttctc
    48241 gagctgatcc gcaccggcag gccggatgaa gaaagctatc tgcacgcggc ggtggaactg
    48301 gcggaagcgc gcggtttgcc cgtcgtggcg accaacgacg tgcgctttat cgacagcagc
    48361 gactttgacg cacacgaaat ccgcgtcgcg atccacgacg gctttaccct cgacgatcct
    48421 aaacgcccgc gtaactattc gccgcagcaa tatatgcgta gcgaagagga gatgtgtgag
    48481 ctgtttgccg acatccccga agcccttgcc aacaccgttg agatcgccaa acgctgtaac
    48541 gtaaccgtgc gtcttggtga atacttcctg ccgcagttcc cgaccgggga catgagcacc
    48601 gaagattatc tggtcaagcg tgcaaaagag ggcctggaag agcgtctggc ctttttattc
    48661 cctgacgagg aagaacgtgt taagcgccgc ccggaatatg acgaacgtct ggagactgaa
    48721 cttcaggtta tcaaccagat gggcttcccg ggctacttcc tcatcgttat ggaatttatc
    48781 cagtggtcga aagataacgg cgtaccggta gggccaggcc gtggctccgg tgcgggttca
    48841 ctggtggcct acgcgctgaa aatcaccgac ctcgatccgc tggaatttga cctgctgttc
    48901 gaacgtttcc ttaacccgga acgtgtctcc atgcctgact tcgacgttga cttctgtatg
    48961 gagaaacgcg atcaggttat cgagcacgta gcggacatgt acggtcgtga tgcggtatcg
    49021 cagatcatca ccttcggtac aatggcggcg aaagcggtga tccgcgacgt aggccgcgtg
    49081 ctggggcatc cgtacggctt tgtcgatcgt atctcgaaac tgatcccgcc cgatccgggg
    49141 atgacgctgg cgaaagcgtt tgaagccgag ccgcagctgc cggaaatcta cgaagcggat
    49201 gaagaagtta aggcgctgat cgacatggcg cgcaaactgg aaggggtcac ccgtaacgcc
    49261 ggtaagcacg ccggtggggt ggttatcgcg ccgaccaaaa ttaccgattt tgcgccgctt
    49321 tactgcgatg aagagggcaa acatccggtc acccagtttg ataaaagcga cgttgaatac
    49381 gccggactgg tgaagttcga cttccttggt ttgcgtacgc tcaccatcat caactgggcg
    49441 ctggagatga tcaacaagcg gcgggcgaag aatggcgagc cgccgctgga tatcgctgcg
    49501 atcccgctgg atgataagaa aagcttcgac atgctgcaac gctcggaaac cacggcggta
    49561 ttccagcttg aatcgcgcgg catgaaggac ctgatcaagc gtctacaacc tgactgcttc
    49621 gaagatatga tcgccctagt ggcactgttc cgccccggtc cgttgcaatc agggatggtg
    49681 gataacttta tcgaccgtaa acatggtcgt gaagagatct cctatccgga cgtacagtgg
    49741 cagcatgaaa gcctgaaacc ggtactggag ccaacctacg gcattatcct gtatcaggaa
    49801 caggtcatgc agattgcgca ggtgctttct ggttataccc tcggtggcgc ggatatgctg
    49861 cgtcgtgcga tgggtaagaa aaagccggaa gagatggcta agcaacgttc tgtatttgct
    49921 gaaggtgcag aaaagaacgg aatcaacgct gaactggcga tgaaaatctt cgacctggtg
    49981 gagaaattcg ctggttacgg atttaacaaa tcgcactctg cggcctatgc tttggtgtca
    50041 tatcaaacgt tatggctgaa agcacactat cctgcggagt ttatggcggc ggtaatgacc
    50101 gccgatatgg acaacaccga gaaggtggtg ggtctggtgg atgagtgctg gcggatgggg
    50161 ctgaaaatcc tgccaccaga tataaactcc ggtctttacc atttccacgt caacgacgac
    50221 ggcgaaatcg tgtatggtat tggcgcgatc aaaggggtcg gtgaaggtcc gattgaggcc
    50281 atcatcgaag cccgtaataa aggcggctac ttccgcgaac tgtttgatct ctgcgcccgt
    50341 accgacacca aaaagttgaa ccgtcgcgtg ctggaaaaac tgatcatgtc cggggcgttt
    50401 gaccgtcttg ggccacaccg cgcggcgctg atgaactcgc tgggtgatgc gttaaaagcg
    50461 gcagatcaac acgcgaaagc ggaagctatc ggtcaggccg atatgttcgg cgtgctggcc
    50521 gaagagccgg aacaaattga acaatcctac gccagctgcc aaccgtggcc ggagcaggtg
    50581 gtattagatg gggaacgtga aacgttaggc ctgtacctga ccggacaccc tatcaaccag
    50641 tatttaaaag agattgagcg ttatgtcgga ggcgtaaggc tgaaagacat gcacccgaca
    50701 gaacgtggta aagtcatcac ggctgcgggg ctcgttgttg ccgcgcgggt tatggtcacc
    50761 aagcgcggca atcgtatcgg tatctgcacg ctggatgacc gttccgggcg gctggaagtg
    50821 atgttgttta ctgacgccct ggataaatac cagcaattgc tggaaaaaga ccgcatactt
    50881 atcgtcagcg gacaggtcag ctttgatgac ttcagcggtg ggcttaaaat gaccgctcgc
    50941 gaagtgatgg atattgacga agcccgggaa aaatatgctc gcgggcttgc tatctcgctg
    51001 acggacaggc aaattgatga ccagctttta aaccgactcc gtcagtctct ggaaccccac
    51061 cgctctggga caattccagt acatctctac tatcagaggg cggatgcacg cgcgcggttg
    51121 cgttttggcg cgacgtggcg tgtctctccg agcgatcgtt tattaaacga tctccgtggc
    51181 ctcattggtt cggagcaggt ggaactggag tttgactaat acaggaatac tatgagtctg
    51241 aatttccttg attttgaaca gccgattgca gagctggaag cgaaaatcga ttctctgact
    51301 gcggttagcc gtcaggatga gaaactggat attaacatcg atgaagaagt gcatcgtctg
    51361 cgtgaaaaaa gcgtagaact gacgcgtaaa atcttcgccg atcttggtgc atggcagatt
    51421 gcgcaactgg cacgccatcc acagcgtcct tataccctgg attacgttcg cctggcattt
    51481 gatgaatttg acgaactggc tggcgaccgc gcgtatgcag acgataaagc tatcgtcggt
    51541 ggtatcgccc gtcttgacgg tcgtccggtg atgatcattg gtcatcaaaa aggtcgtgaa
    51601 accaaagaaa aaattcgccg taactttggt atgccagcgc cagaaggtta ccgcaaagca
    51661 ctgcgtctga tgcaaatggc tgaacgcttt aagatgccta tcatcacctt tatcgacacc
    51721 ccgggggctt atccgggcgt gggcgcagaa gaacgtggtc agtctgaagc cattgcacgc
    51781 aacctgcgtg aaatgtctcg cctcggtgta ccggtagttt gtacggttat cggtgaaggt
    51841 ggttctggcg gcgcgctggc gattggcgtg ggcgataaag tgaatatgct gcaatacagc
    51901 acctattccg ttatctcgcc ggaaggttgt gcgtccattc tgtggaagag cgctgacaaa
    51961 gcgccgctgg cagctgaagc gatgggtatc atcgctccgc gtctgaaaga actaaaactg
    52021 atcgactcca tcatcccgga accgctgggt ggtgctcacc gtaacccgga agcgatggcg
    52081 gcatcgttga aagcgcaact gctggcggat ctggccgatc tcgacgtgtt aagcactgaa
    52141 gatttaaaaa atcgtcgtta tcagcgcctg atgagctacg gttacgcgta attcgcaaaa
    52201 gttctgaaaa agggtcactt cggtggccct tttttatcgc cacggtttga gcaggctatg
    52261 attaaggaag gattttccag gaggaacaca tgaacatcat tgccattatg ggaccgcatg
    52321 gcgtctttta taaagatgag cccatcaaag aactggagtc ggcgctggtg gcgcaaggct
    52381 ttcagattat ctggccacaa aacagcgttg atttgctgaa atttatcgaa cataaccctc
    52441 gaatttgcgg cgtgattttt gactgggatg agtacagtct cgatttatgc agcgatatca
    52501 atcaacttaa tgaatatctc ccgctttatg ccttcatcaa cacccactcg acgatggatg
    52561 tcagcgtgca ggatatgcgg atggcgctct ggttttttga atatgcgctg gggcaggcgg
    52621 aagatatcgc cattcgtatg cgtcagtaca ccgacgaata tcttgataac attacaccgc
    52681 cgttcacgaa agccttgttt acctacgtca aagagcggaa gtacaccttt tgtacgccgg
    52741 ggcatatggg cggcaccgca tatcaaaaaa gcccggttgg ctgcctgttt tatgattttt
    52801 tcggcgggaa taccctcaag gctgatgtct ctatttcggt caccgagctt ggttcgttgc
    52861 tcgaccacac cgggccacac ctggaagcgg aagagtacat cgcgcggact tttggcgcgg
    52921 aacagagtta tatcgttacc aacggaacat cgacgtcgaa caaaattgtg ggtatgtacg
    52981 ccgcgccatc cggcagtacg ctgttgatcg accgcaattg tcataaatcg ctggcgcatc
    53041 tgttgatgat gaacgatgta gtgccagtct ggctgaaacc gacgcgtaat gcgttgggga
    53101 ttcttggtgg gatcccgcgc cgtgaattta ctcgcgacag catcgaagag aaagtcgctg
    53161 ccaccacgca ggcacaatgg ccggttcatg cggtgattac caactccacc tatgatggct
    53221 tgctctacaa caccgactgg atcaaacaga cgctggatgt cccgtcgatc cacttcgatt
    53281 ccgcctgggt gccgtacacc cattttcatc cgatctacca ggggaaaagt ggtatgagcg
    53341 gcgagcgtgt tgcgggaaaa gtgatcttcg aaacgcaatc gacccacaaa atgctggcgg
    53401 cgttatcgca ggcttcgctg atccacatta aaggcgagta tgacgaagag gcgtttaacg
    53461 aagcctttat gatgcatacc accacctcgc ccagttatcc cattgttgct tcggttgaga
    53521 cggcggcggc gatgctgcgt ggtaatccgg gcaaacggct gattaaccgt tcggtggagc
    53581 gggcgctgca ttttcgcaaa gaggtccagc ggttgcggga agagtctgac ggttggtttt
    53641 tcgatatctg gcaaccgccg caggtggatg aagccgaatg ctggcccgtt gcgctaggcg
    53701 aacagtggca cggctttaac gatgcggatg ccgatcatat gtttctcgat ccggttaaag
    53761 tcactatttt gacaccgggg atggacgaac agggcaatat gagcgaggag gggatcccgg
    53821 cggcgctggt ggcaaaattc ctcgacgaac gtgggatcgt agtagagaaa accggccctt
    53881 ataacctgct gtttctcttt agtattggta tcgataaaac caaagcaatg ggattattgc
    53941 gtgggttgac ggaatttaaa cgctcttacg atctcaacct gcggatcaaa aatatgctac
    54001 ccgatctcta tgctgaagat cccgatttct accgcaatat gcgtattcag gatctggcgc
    54061 aagggatcca taagctgatt cgtaaacacg atcttcccgg tttgatgttg cgggcattcg
    54121 atactttgcc ggaaatgatc atgacgccac atcaggcatg gcagagacag attaaaggtg
    54181 aagtagaaac cattgcgctg gaacaactgg tcggtagagt atcggcaaat atgatcctgc
    54241 cttatccacc gggcgtaccg ctgttgatgc caggagaaat gctgaccaaa gagagccgca
    54301 cggtactcga ttttctactg atgctttgtt ccgtagggca acattacccc ggttttgaaa
    54361 cggatattca cggcgcgaaa caggacgaag acggcgttta ccgcgtacga gtcctaaaaa
    54421 tggcgggata acttgccaga gcggcttccg ggcgagtaac gtgctgttaa caaataaagg
    54481 agacgttatg ctgggtttaa aacaggttca ccatattgcg attattgcga cggattatgc
    54541 ggtgagcaaa gctttctact gcgatattct tggtttcacg ctgcaaagcg aagtctatcg
    54601 cgaagcgcgc gactcgtgga aaggggattt ggcgcttaat gggcaatatg tgattgagct
    54661 tttctctttt ccgttcccgc cggagcgccc cagccgaccg gaagcttgcg gcctgcgtca
    54721 tctggctttt agcgttgatg acatcgatgc ggcagtggcg caccttgaaa gccataacgt
    54781 gaagtgtgaa gccatccgtg tcgatccata cacgcaaaaa cgcttcacct tctttaacga
    54841 tccggacggg ctgccgttgg aactgtatga gcagtaaggc ttgtcatcgc cgcatttgcc
    54901 cggtaacgtg ccgggcattg ctactgtaaa atcgcaccat catgacactc acgctcaata
    54961 gacaacttct cacctcacgc cagattctgg tggcctttag cggcgggctt gactccaccg
    55021 ttctgctgca tcagttggtg cagtggcgga cggaaaatcc gggcgtcact ctgcgcgcta
    55081 ttcatgtgca tcacggttta agtgccaatg ccgatgcctg ggttacgcat tgcgaaaacg
    55141 tctgccaaca gtggcaggtg ccgctggtgg tcgaacgcgt gcaacttgcg caagaaggac
    55201 tgggcattga ggcccaggcg cggcaggcgc gttatcaggc atttgcccgc accttgttgc
    55261 ccggtgaagt gctggtcacc gcgcaacatc tcgacgatca atgtgaaact tttctgctgg
    55321 cgctaaaacg cggtagcggc cctgccgggc tttcggctat ggcggaagtt tcggagtttg
    55381 ccggaacacg gcttatccgc ccgttgctcg cccgcacgcg gggagaactg gagcagtggg
    55441 cattggcgca tggtttacgc tggattgaag acgaaagtaa tcaggacgac tcatacgatc
    55501 gtaactttct gcgcctgcgc gtagtgccgt tattgcagca gcgttggccg cattttgcgg
    55561 aagcaacggc ccgcagcgcc gcactttgtg ctgaacaaga gagtctgttg gatgaactgc
    55621 tggcggatga tttagcacac tgccaaacgt cgcaggggac gctgcagatt gcgccaatgc
    55681 tggcgatgag tgatgcccgc cgcgcggcga ttatccgtcg ctggctggca gggcagaatg
    55741 caccaatgcc ttcccgcgac gcgctggtgc ggatctggca ggaagtggcg ctggcgcggg
    55801 aagatgcctc cccctgttta cgcttgggcg cgtttgaaat ccgccgctat cagtcgcaac
    55861 tgtggtggat taaatccgtc accgggcaaa gtgaaaccat tgtgccgtgg cagacgtggc
    55921 tacaaccgct ggaattaccg gcggggctgg gaagcgtaca gcttactgcg ggaggcgata
    55981 ttcgccctcc gcgtgcagac gaagcggtca gcgtgcgttt caaagcggca ggattgctgc
    56041 atattgtcgg gcgcaacggc ggacgtaagc tgaagaaaat ctggcaagag ttaggcgtgc
    56101 cgccgtggct acgtgacacc acgccactgc tgttttatgg cgaaacgctg attgcggcgg
    56161 caggggtatt tgtgacgcaa gaaggtgtgg ctgaaggtga gaatggcgta agttttgtct
    56221 ggcagaaaac gcttagttaa gtgaaagccg gataagacgc atcaaacgtc gcatccggcg
    56281 aaagtcaatc aggactcgct caccaccacc gtaccgattt ccgggtggct aaagctggta
    56341 attttatcca gacgtagctc acgggtttcg ccagcggcct cgacgaccag atactccaca
    56401 tttttgcggg agactaaatc gctggctttt gcctgcaatt tttcgccatc tttcagctca
    56461 agtgtcagca ttaaatgatg ctggcaggcg agctcaagat tatcgtaatc atcacaattg
    56521 attggttgat acgtatcatt cattgacata atcgctcacc agtaagtttg ccgcagcgta
    56581 tgctgctttt tccctgacag cctcagaaag ggcgtcgtcg gcagccattt cattcagcac
    56641 tttcaaaacg cagcccagcg cgtccggaac gtatcctaag tctccgctgg cgatttccgc
    56701 gtaccgcttg cgtattaact cacaatattt ttccacatgc cctcctgtca gcactctgac
    56761 ttaaccgtgg atgcaagtct aagcctacga agataaactc tgtttcgcaa ggtgactata
    56821 ccacactcat gtctgcaata tcagcgccgc aactgcacgt attccgttac aatggcctcc
    56881 tgattcgaaa ggagttttct tatggcgctt aaagcgacaa tttataaagc gacggttaat
    56941 gtggccgatc tcgaccgcaa ccagtttctc gatgcctctc tgacgctggc gcgccaccct
    57001 tcagaaaccc aggagcgtat gatgctgcgc ttgctggcgt ggctgaaata tgctgatgaa
    57061 cgtctgcaat ttacccgtgg tttgtgtgcc gatgatgagc cggaagcgtg gctgcgtaac
    57121 gatcacctgg gcattgattt gtggattgag ctggggctgc cggatgagcg gcggattaag
    57181 aaagcctgca cccaggccgc agaagtggcg ctgtttacct ataatagtcg ggcggcgcaa
    57241 atctggtggc agcaaaatca gagcaaatgt gtgcagtttg ccaatctttc cgtctggtat
    57301 ctggacgatg aacaactggc gaaagtaagc gcctttgccg atcgtaccat gacgctgcag
    57361 gcaacgattc aggatggcgt gatctggtta tcggacgata agaataatct ggaagttaac
    57421 ttaaccgcct ggcaacaacc ttcatgattg tgatttcccg acatgttgct attcccgatg
    57481 gtgagcttga gatcaccgcc attcgtgcgc agggcgcggg cgggcagcat gttaataaga
    57541 cctcaacggc cattcatctg cgttttgaca ttcgggcgtc cagcctgcca gagtattaca
    57601 aagagcgtct actcgccgcc agccatcatt tgatcagcag tgatggcgtg attgtcatta
    57661 aggcacagga ataccgcagt caggaactga accgcgaagc cgcgctagcc cggctggtgg
    57721 cagtgattaa agatttaaca acagaacaaa aagcccgacg acccacgcgg cccacccgtg
    57781 catcgaaaga gcgcaggctg gcatcgaaag cacaaaaatc aagcgtgaag gcgatgcgcg
    57841 gcaaagtacg cagcggtcgg gaataaaaag aaggaataga tggtgaaaaa agcgatagtg
    57901 acagcgatgg ctgtaatcag cctctttact ctgatgggat gtaataaccg ggccgaagtc
    57961 gatacgcttt ctccagcaca ggctgccgaa ctgaaaccga tgccgcaaag ttggcgcggc
    58021 gtgctgccgt gtgctgattg cgaaggaatc gaaacctctc tgttcctcga aaaagacgga
    58081 acatgggtga tgaatgagcg ttatctcggt gctcgtgaag aaccttcctc cttcgcttcc
    58141 tacggtacat gggcgcgaac cgctgacaag ctggtattaa ccgacagcaa aggtgaaaag
    58201 tcatattatc gggcgaaagg cgatgcgctg gagatgctcg atcgtgaagg caatccgatt
    58261 gaatcgcagt tcaactatac gctggaaccg gcacaatcta gtttacctat gacgccgatg
    58321 accctgcgag gcatgtattt ttatatggct gatgcggcga ccttcactga ttgcgcgacc
    58381 ggaaaacgtt tcatggtagc gaataacgca gagctggagc gtagctacct ggctgcgcgc
    58441 ggtcacagtg aaaaaccggt gttactgtca gtagaaggtc actttacgct tgaggctaat
    58501 ccggatactg gtgcgccaac caaagtatta gcgcccgata cggcgggtaa attttacccg
    58561 aataaggatt gcagtagttt ggggcagtaa acctgcttaa ggcagaaaca aacgcaa
//
LOCUS       DAZGJW010000012        58585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZGJW010000012 DAZGJW010000000
VERSION     DAZGJW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.012"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..372
                     /locus_tag="ACHKIK_000786"
     CDS             <1..372
                     /locus_tag="ACHKIK_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFO9082013.1"
                     /translation="NSTPSTGYQPLILQSIKTKLLSSRLNKFNDFEDSFNGLGISVHD
                     ISAQKISLLSFQKYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHK
                     DFAFKPFFTNFNTIERIENYL"
     gene            369..861
                     /locus_tag="ACHKIK_000787"
                     /pseudo
     CDS             369..861
                     /locus_tag="ACHKIK_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
     gene            complement(903..1853)
                     /gene="corA"
                     /locus_tag="ACHKIK_000788"
     CDS             complement(903..1853)
                     /gene="corA"
                     /locus_tag="ACHKIK_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO9082014.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(2005..2061)
                     /gene="ysgD"
                     /locus_tag="ACHKIK_000789"
     CDS             complement(2005..2061)
                     /gene="ysgD"
                     /locus_tag="ACHKIK_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO9082015.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2223..2987
                     /gene="yigE"
                     /locus_tag="ACHKIK_000790"
     CDS             2223..2987
                     /gene="yigE"
                     /locus_tag="ACHKIK_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO9082016.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(3134..5296)
                     /gene="uvrD"
                     /locus_tag="ACHKIK_000791"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(3134..5296)
                     /gene="uvrD"
                     /locus_tag="ACHKIK_000791"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO9082017.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(5380..6096)
                     /gene="yigB"
                     /locus_tag="ACHKIK_000792"
     CDS             complement(5380..6096)
                     /gene="yigB"
                     /locus_tag="ACHKIK_000792"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO9082018.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREVEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(6096..6992)
                     /gene="xerC"
                     /locus_tag="ACHKIK_000793"
     CDS             complement(6096..6992)
                     /gene="xerC"
                     /locus_tag="ACHKIK_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO9082019.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(6989..7696)
                     /gene="yigA"
                     /locus_tag="ACHKIK_000794"
     CDS             complement(6989..7696)
                     /gene="yigA"
                     /locus_tag="ACHKIK_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFO9082020.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(7693..8517)
                     /gene="dapF"
                     /locus_tag="ACHKIK_000795"
     CDS             complement(7693..8517)
                     /gene="dapF"
                     /locus_tag="ACHKIK_000795"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO9082021.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(8554..8757)
                     /gene="yifL"
                     /locus_tag="ACHKIK_000796"
     CDS             complement(8554..8757)
                     /gene="yifL"
                     /locus_tag="ACHKIK_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO9082022.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            9107..9661
                     /locus_tag="ACHKIK_000797"
     CDS             9107..9661
                     /locus_tag="ACHKIK_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO9082023.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            9999..11507
                     /locus_tag="ACHKIK_000798"
     CDS             9999..11507
                     /locus_tag="ACHKIK_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO9082024.1"
                     /translation="MIGTSRQDGFMHYRNVVCLLSCSLFLSSAWGCRLDPPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKR
                     TDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPDSFRVLAQK
                     SWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLEWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQ
                     FQPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            11504..12829
                     /locus_tag="ACHKIK_000799"
     CDS             11504..12829
                     /locus_tag="ACHKIK_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO9082025.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTSDRQWSKWIDLPDIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTHVREENVRELHNRDIIIPLTDDSCRNAATDGQTLKP"
     gene            12877..13197
                     /gene="cyaY"
                     /locus_tag="ACHKIK_000800"
     CDS             12877..13197
                     /gene="cyaY"
                     /locus_tag="ACHKIK_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO9082026.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(13237..15783)
                     /gene="cyaA"
                     /locus_tag="ACHKIK_000801"
     CDS             complement(13237..15783)
                     /gene="cyaA"
                     /locus_tag="ACHKIK_000801"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO9082027.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDNDTPLLQQYFS"
     gene            16170..17111
                     /gene="hemC"
                     /locus_tag="ACHKIK_000802"
     CDS             16170..17111
                     /gene="hemC"
                     /locus_tag="ACHKIK_000802"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO9082028.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            17108..17848
                     /gene="hemD"
                     /locus_tag="ACHKIK_000803"
     CDS             17108..17848
                     /gene="hemD"
                     /locus_tag="ACHKIK_000803"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO9082029.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            17870..19099
                     /gene="hemX"
                     /locus_tag="ACHKIK_000804"
     CDS             17870..19099
                     /gene="hemX"
                     /locus_tag="ACHKIK_000804"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO9082030.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPQGDTPAAAPQGE"
     gene            19102..20298
                     /gene="hemY"
                     /locus_tag="ACHKIK_000805"
     CDS             19102..20298
                     /gene="hemY"
                     /locus_tag="ACHKIK_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO9082031.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            20977..21114
                     /locus_tag="ACHKIK_000806"
                     /pseudo
     CDS             20977..21114
                     /locus_tag="ACHKIK_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(21222..22457)
                     /gene="aslB"
                     /locus_tag="ACHKIK_000807"
     CDS             complement(21222..22457)
                     /gene="aslB"
                     /locus_tag="ACHKIK_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO9082032.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(22604..22680)
                     /locus_tag="ACHKIK_000808"
     tRNA            complement(22604..22680)
                     /locus_tag="ACHKIK_000808"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22644..22646),aa:Pro,seq:tgg)
     gene            complement(22723..22809)
                     /locus_tag="ACHKIK_000809"
     tRNA            complement(22723..22809)
                     /locus_tag="ACHKIK_000809"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22773..22775),aa:Leu,seq:cag)
     gene            complement(22830..22905)
                     /locus_tag="ACHKIK_000810"
     tRNA            complement(22830..22905)
                     /locus_tag="ACHKIK_000810"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22870..22872),aa:His,seq:gtg)
     gene            complement(22964..23040)
                     /locus_tag="ACHKIK_000811"
     tRNA            complement(22964..23040)
                     /locus_tag="ACHKIK_000811"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23004..23006),aa:Arg,seq:ccg)
     gene            complement(23143..24528)
                     /gene="yifK"
                     /locus_tag="ACHKIK_000812"
     CDS             complement(23143..24528)
                     /gene="yifK"
                     /locus_tag="ACHKIK_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9082033.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(24719..25459)
                     /gene="wecG"
                     /locus_tag="ACHKIK_000813"
     CDS             complement(24719..25459)
                     /gene="wecG"
                     /locus_tag="ACHKIK_000813"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO9082034.1"
                     /translation="MNNNTMAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(25462..26814)
                     /gene="wzyE"
                     /locus_tag="ACHKIK_000814"
     CDS             complement(25462..26814)
                     /gene="wzyE"
                     /locus_tag="ACHKIK_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO9082035.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(26811..27890)
                     /gene="rffT"
                     /locus_tag="ACHKIK_000815"
                     /gene_synonym="wecF"
     CDS             complement(26811..27890)
                     /gene="rffT"
                     /locus_tag="ACHKIK_000815"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO9082036.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDLSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEGA"
     gene            complement(27887..29137)
                     /gene="wzxE"
                     /locus_tag="ACHKIK_000816"
     CDS             complement(27887..29137)
                     /gene="wzxE"
                     /locus_tag="ACHKIK_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO9082037.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPATIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(29139..30269)
                     /gene="rffA"
                     /locus_tag="ACHKIK_000817"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(29139..30269)
                     /gene="rffA"
                     /locus_tag="ACHKIK_000817"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO9082038.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(30274..30948)
                     /gene="rffC"
                     /locus_tag="ACHKIK_000818"
                     /gene_synonym="wecD"
     CDS             complement(30274..30948)
                     /gene="rffC"
                     /locus_tag="ACHKIK_000818"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO9082039.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(30926..31807)
                     /gene="rfbA"
                     /locus_tag="ACHKIK_000819"
     CDS             complement(30926..31807)
                     /gene="rfbA"
                     /locus_tag="ACHKIK_000819"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO9082040.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(31826..32893)
                     /gene="rffG"
                     /locus_tag="ACHKIK_000820"
     CDS             complement(31826..32893)
                     /gene="rffG"
                     /locus_tag="ACHKIK_000820"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO9082041.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(32893..34155)
                     /gene="wecC"
                     /locus_tag="ACHKIK_000821"
     CDS             complement(32893..34155)
                     /gene="wecC"
                     /locus_tag="ACHKIK_000821"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO9082042.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(34152..35282)
                     /gene="wecB"
                     /locus_tag="ACHKIK_000822"
     CDS             complement(34152..35282)
                     /gene="wecB"
                     /locus_tag="ACHKIK_000822"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO9082043.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(35338..36384)
                     /gene="wzzE"
                     /locus_tag="ACHKIK_000823"
     CDS             complement(35338..36384)
                     /gene="wzzE"
                     /locus_tag="ACHKIK_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO9082044.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNEELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(36396..37499)
                     /gene="wecA"
                     /locus_tag="ACHKIK_000824"
     CDS             complement(36396..37499)
                     /gene="wecA"
                     /locus_tag="ACHKIK_000824"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO9082045.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(37739..38998)
                     /gene="rho"
                     /locus_tag="ACHKIK_000825"
     CDS             complement(37739..38998)
                     /gene="rho"
                     /locus_tag="ACHKIK_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO9082046.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(39083..39184)
                     /gene="rhoL"
                     /locus_tag="ACHKIK_000826"
     CDS             complement(39083..39184)
                     /gene="rhoL"
                     /locus_tag="ACHKIK_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO9082047.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(39325..39654)
                     /gene="trxA"
                     /locus_tag="ACHKIK_000827"
     CDS             complement(39325..39654)
                     /gene="trxA"
                     /locus_tag="ACHKIK_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO9082048.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            39785..41050
                     /gene="rhlB"
                     /locus_tag="ACHKIK_000828"
     CDS             39785..41050
                     /gene="rhlB"
                     /locus_tag="ACHKIK_000828"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO9082049.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKYRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            41184..42668
                     /gene="gppA"
                     /locus_tag="ACHKIK_000829"
     CDS             41184..42668
                     /gene="gppA"
                     /locus_tag="ACHKIK_000829"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO9082050.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(42715..44736)
                     /gene="rep"
                     /locus_tag="ACHKIK_000830"
     CDS             complement(42715..44736)
                     /gene="rep"
                     /locus_tag="ACHKIK_000830"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO9082051.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            44953..45402
                     /locus_tag="ACHKIK_000831"
     CDS             44953..45402
                     /locus_tag="ACHKIK_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO9082052.1"
                     /translation="MAINFSPKVGEIQECNFGNYPVIQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            45601..45882
                     /gene="ppiC"
                     /locus_tag="ACHKIK_000832"
     CDS             45601..45882
                     /gene="ppiC"
                     /locus_tag="ACHKIK_000832"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO9082053.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(45969..47444)
                     /gene="ilvC"
                     /locus_tag="ACHKIK_000833"
     CDS             complement(45969..47444)
                     /gene="ilvC"
                     /locus_tag="ACHKIK_000833"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO9082054.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            47594..48487
                     /gene="ilvY"
                     /locus_tag="ACHKIK_000834"
     CDS             47594..48487
                     /gene="ilvY"
                     /locus_tag="ACHKIK_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO9082055.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSAEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(48539..50083)
                     /gene="ilvA"
                     /locus_tag="ACHKIK_000835"
     CDS             complement(48539..50083)
                     /gene="ilvA"
                     /locus_tag="ACHKIK_000835"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO9082056.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(50086..51936)
                     /gene="ilvD"
                     /locus_tag="ACHKIK_000836"
     CDS             complement(50086..51936)
                     /gene="ilvD"
                     /locus_tag="ACHKIK_000836"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO9082057.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQDARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(52001..52930)
                     /gene="ilvE"
                     /locus_tag="ACHKIK_000837"
     CDS             complement(52001..52930)
                     /gene="ilvE"
                     /locus_tag="ACHKIK_000837"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO9082058.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(52950..53213)
                     /gene="ilvM"
                     /locus_tag="ACHKIK_000838"
     CDS             complement(52950..53213)
                     /gene="ilvM"
                     /locus_tag="ACHKIK_000838"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO9082059.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(53210..54856)
                     /gene="ilvG"
                     /locus_tag="ACHKIK_000839"
     CDS             complement(53210..54856)
                     /gene="ilvG"
                     /locus_tag="ACHKIK_000839"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO9082060.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(54859..54909)
                     /gene="ilvX"
                     /locus_tag="ACHKIK_000840"
     CDS             complement(54859..54909)
                     /gene="ilvX"
                     /locus_tag="ACHKIK_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO9082061.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(54996..55094)
                     /gene="ilvL"
                     /locus_tag="ACHKIK_000841"
     CDS             complement(54996..55094)
                     /gene="ilvL"
                     /locus_tag="ACHKIK_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO9082062.1"
                     /translation="MTALLRVISLVMISVVVIIIPPCGAALGRGKA"
     gene            55447..56967
                     /gene="yifB"
                     /locus_tag="ACHKIK_000842"
     CDS             55447..56967
                     /gene="yifB"
                     /locus_tag="ACHKIK_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO9082063.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(56992..57330)
                     /gene="maoP"
                     /locus_tag="ACHKIK_000843"
     CDS             complement(56992..57330)
                     /gene="maoP"
                     /locus_tag="ACHKIK_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO9082064.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            57449..58288
                     /gene="hdfR"
                     /locus_tag="ACHKIK_000844"
     CDS             57449..58288
                     /gene="hdfR"
                     /locus_tag="ACHKIK_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO9082065.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(58384..58459)
                     /locus_tag="ACHKIK_000845"
     tRNA            complement(58384..58459)
                     /locus_tag="ACHKIK_000845"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58424..58426),aa:Trp,seq:cca)
     gene            complement(58468..58544)
                     /locus_tag="ACHKIK_000846"
     tRNA            complement(58468..58544)
                     /locus_tag="ACHKIK_000846"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58508..58510),aa:Asp,seq:gtc)
BASE COUNT        14476 a        15660 c        14792 g        13657 t
ORIGIN      
        1 aattcaactc cctcgacagg ataccaacct cttatcctgc aaagcattaa aaccaaatta
       61 ctttcatcaa gactaaataa atttaatgac ttcgaggaca gttttaatgg cttaggaatc
      121 agtgttcatg atatttcagc tcaaaaaata tctcttttaa gttttcaaaa atacgctata
      181 ggatggagtg caacaattca ttttgtagca caggaccact tcggtcttga tgtaactgat
      241 atcaaaaaca agacttacag caaatataga ttttttcgca tatggttctt tttacaacgg
      301 cataaagact ttgctttcaa accttttttc accaatttta acactatcga aagaatcgaa
      361 aattatctat gaaaagcaaa ctccagaaaa ttatattatg tttatttctt ctatgctgca
      421 tctataattt atggacattg cgtcctgtac agattttgta tacatactct gatgctggca
      481 attcagtctt tctggtcgtt gatcatctgc catggactga cagcgataaa ataaattggt
      541 atctgaagca tcagaatgaa attaaaaatc aacatcccct accagaaggt agttggcata
      601 cctggtatgt cattgatatc ggtaatggct tcactgacta taaaaaaata tatcgaaggg
      661 ccgtatgaag atctctactg tttcccgaca attaagagca atgataattg catcgtgaaa
      721 aattacttaa tggttattaa tgaataccct tatcgtaaca ctcatattgg tattaacgat
      781 tttactgagt atcaattaac acaagaaaat aaaatagagc gagttttcaa tccgcatgac
      841 tttaaaaaag acaattttta gaaataagcc ggataaaaaa atagctttat ccggcactca
      901 tattacaacc agttcttccg cttaaagtac agatacggtg ccaggcccgc gaggatcata
      961 aagataatcg cgcctgggta gccgaaactc cacttcaact ctggcataaa ttcaaagttc
     1021 atcccgtagc tggaagccac cagcgtcggt ggcaggaaca ccacggatac caccgagaag
     1081 attttgatga tgcggttctg ctcgatgttg ataaagccca tcgccgcctg catcaggaag
     1141 ttcaccttct ggaacaggga ttcgttatgc ggcagcaggg attcgatatc tcgcaggatt
     1201 tcacgcgcct gctccagttg cccacccggt aaacgcgcct tgcgtaccag gaagttgagc
     1261 gcgcgctggg tatccatcag acacaggcga actttccagc cgatatcttc cagttccgcc
     1321 agcgtggaga gcgcctcgtc gtactcatcg ccctgatgcc cttccataat cacccggctc
     1381 aactgctcca ggtcgctata gatattttca atttcatctg ccaactgttc gattttggtt
     1441 tcgaacagat ccagcagcaa ctcgtaggcg ttaccgtcta ccatcgactg gctacgggca
     1501 cgcatacgat acagacgaaa agcgggcagt tcacgctcac gcagagtaaa cagacgacca
     1561 tcacggatgg taaatgccac agtggagtta ccggcgtgat cttccgcatc ttcaaagaag
     1621 aagaaggagt gaatatgcag gccgtcgtcg tcttcaaaga aacgtgccga tgcttcgatg
     1681 tcttccagtt ccgggcgggt tgccaggctc tggccaagtt cagattgtac gcgcagtcgc
     1741 tcgtcgtcgt ccggttcgac aagatcaatc catactgcat ttacaagggg ttgtgactct
     1801 tcgacttcca gccgggtcag tcggttattt tccagttgaa atgcgctcag catgaccggg
     1861 actcccaatg cttaaaatat cggacagttc ggtgggcaca cagaaacaaa ttgggtttca
     1921 gaccattaaa cagcctgact cagcgcgacg ggaaaaattg aggtcgctga caaccgctaa
     1981 ggctatcagc aaaaagggat agccttagga gttgatcctg gatgacagga tagtgagcca
     2041 gtatctactg ggtgtgtcca aggcgaatgt cctcttagag tgatcgtgcg cgcatgttac
     2101 gccagcaaaa ttttgccgtc aacacgcaac agaacaccac agaacaatag ttgaacttcg
     2161 cgctttttgg tactcgtcaa ggtcgctaac aggataaaaa aatccaacat ccaggaccta
     2221 taatggcgca tcggctactc attggtaaag gaatgatcac cttgaatctc aaacggattt
     2281 ttctcaccct caccttactt cccttgtttg ctgttgccgc tgatgattgc gcactctcag
     2341 atccgacgct gaccgtacag gcgtataccg ttaatcctca aacagagcgg gtgaaaatgt
     2401 actggcaaaa agccaatggc gaagcgtggg gaactttaca tgctctgctg gcggatatga
     2461 atagtcaggg tcaggtgcag atggcgatga acggcggcat ctatgatgaa agctatgcgc
     2521 cgctcggttt gtacatcgaa aacggtcagc agaaggtggc gttaaatctc gcttcaggtg
     2581 aagggaattt ctttatccgt cctggcggcg tgttttatgt cgcgggagat aaagtcggca
     2641 tcgttcgtct ggatgccttc aaaaccagta aagagattca gtttgcggtg cagtcagggc
     2701 caatgttgat ggaaaacagt gtaattaatc cgcgtattca tcccaacgtc gcctcacgta
     2761 aaattcgtaa cggtgttggg attaataaac atgggaatgc cgtgtttttg ttgagccagc
     2821 aggcgacaaa tttttatgat tttgcctgtt atgccaaagc gaaactgaac gttgagcagc
     2881 tgctttatct tgatggcacg atttcacata tgtatatgaa aggcggggca atcccgtggc
     2941 aacgctatcc ttttgtcacc atgatttccg tagagcggaa aggttaaaac gatagttttg
     3001 tgtcagatac tgaagatggc gcagatgccg gatgcggcgt aaacgcccta tccggcctac
     3061 atgacgttgc aatttattga atctgaatga ttttttaggc caagtaaggt gcgcagcacc
     3121 gcatccggca acgttacacc gactccagcc gggcataagc cgccaccagc catttaatac
     3181 cctggccctg aaatgccacc tgcaaacggc tatgctcacc gctgccttcc atattgacaa
     3241 tggtgccttc accaaactta gcgtggcgta cgcgctggcc gagcttgtag ccgctgtcgt
     3301 tctcgaccat cggcgtaccc atccgctgat ggctgaccgg gcggcttacc gtggcgcgca
     3361 ggcgcacctc ttccacacac tcttccggca gctcgccaat aaagcgcgac gggcgatggt
     3421 aaacctcttt gccatacaga cggcgagttt ccgcgtaggt cagcgtcagt ttctgcatcg
     3481 cgcgggttac gccaacgtag gccagacggc gttcttcttc cagacgcccg ccttcatcca
     3541 gcgacatctg gcttgggaac atgccctctt ccataccgac gataaacacc tgcgggaact
     3601 ccagaccttt cgccgagtgc atggtcatca gttgtaccgc atcttgccag gtatccgcct
     3661 gcccttcgcc tgcttccagc gccgcatggg ataggaacgc ctgcagcggc attaaatctt
     3721 cgtcttcttc gttgtagctg aactggcgcg ttgccgtcac cagttcctct aagttttcga
     3781 tacgcgtctg acctttttcg cccttctcct gctcgtacat ggtacgcagg ccggagtctt
     3841 taattacccg gtcagtctgt acatgcagcg gcatatcggc agtttcctgc gctaaggcgt
     3901 cgatcagttc cataaaccgc tgtaaggcgc tggcagcacg tccggcgagg gctttttcct
     3961 gcaacagttc acgacatgcc tgccagagtg ttaactggcg atcgcgcgat gtctgacgta
     4021 ccacgtccag cgtccggtca ccaatacccc gcgttggcgt attcaccaca cgctcaaagg
     4081 ccgcgtcgtc gttacggttg gcaatcaggc gcagatacga gagcgcatct ttgatttcct
     4141 ggcgttcgaa gaagcgcatc ccgccgtaaa tacggtacgg catactcgcc tgcaacagtg
     4201 cttcttccag cacacgcgac tgggcgttac tgcggtagag aatggcgcac tcggcaagcg
     4261 ccccgccgtt gtcctgccag gttttgatgc ggttaaccac aaaacgcgct tcatcgagtt
     4321 cgttaaaagc gcaatagagg gaaataggct caccgtccgc gccatcggtc cacagttttt
     4381 tacccaaacg cccgttgttg ttttcaatca gggcgttcgc ggcgctcaga atattgctgg
     4441 tagagcggta gttctgctcc agacggatag tttcagcacc ggggaaatca ttgaggaaac
     4501 gctgaatatt ctccacctgc gctccacgcc agccgtagat tgactgatcg tcatcgccaa
     4561 cgatcatcac tttgccggta tcgcccgcca gcaggcggat ccacgcatac tgaatgttgt
     4621 tggtatcctg gaattcgtcc accaggatat tggtaaaccg ttcgcgatag tgctgaagga
     4681 tatgcggctt gttaagccac aactcgtgag cacgtagcag cagctcggcg aagtccacca
     4741 ggcccgcgcg gtcacacgct tcctgatacg cctgatacac cttctgccag gtctgctcca
     4801 ccggattacc gtagctttga atatgatgtg gacgcaggcc ttcatctttc tggctgttga
     4861 tgtaccacat tgcctggcgc ggcggccact gcttctcgtc gaggttcatc gccttgatta
     4921 ggcgtttgag caggcgtagc tggtcctcgc tgtcgaggat ctgaaaatcc tgcggcagat
     4981 tggcgtccat atggtgcgca cgcagcagac ggtgcgccag cccgtggaag gtgccgaccc
     5041 acatgccgcc ctggctggtg cccatcagct gcccgatacg atgacgcatc tccgccgccg
     5101 ctttgttggt aaacgtcacc gccataatcg agtatggcga gcagttttcc acgctcatca
     5161 gccaggcgat acgatgcacc agtacgcgcg tcttaccact gcccgcgccc gccagcacca
     5221 gaaggttgct gcgtggcgcg gccaccgctt cgcgctgttt gtcattaagg ctgtcgagca
     5281 ggtaagaaac gtccattggc accgccgcgt aaaaataggt tgggcggaga agcgcaacgc
     5341 cctccgccaa aaagctgggt atatatacag atttgctgat tatatcagcg aggtcagaga
     5401 tgccaaccgg gaaatttcca gatgcggcag taaacggctg tcccaggttt gcatcagatc
     5461 gccattttcc ggtctgatcc aacaggcctg cattccgctg cgaattgccc cacccacgtc
     5521 agtggtgaga tcgtccccaa catgtaagat ctcgccgatc ggcacgttga gtttttccgc
     5581 agccagaaag tacatatcgc tgaacggttt tgagcgcccg tgcgggccag cgcgcagcac
     5641 aaactcaaaa taatccccca aaccaaacag ttccggctgg gcgttaccgt tggtgatcgc
     5701 caccagcggc catttcttcg ccagctgttt taaggtgtcg tgagtttgct gcgggacgtc
     5761 gattcggctg cgccatttgg caaagttgat cattgctgcg tgtgcgcctg cactggcttc
     5821 ttcggcactc agcccggcgt cgagcatcgc ttgttcaatc gaacgaaaac gccagcgcgt
     5881 cacgtcgtga taaatctcgg gttccacttc ccgtaccgcc tggcgcaggc gttgcagatc
     5941 ttcattctgg aagctgcgca gcgccggatg ataattttgc acaaaggtaa gcgcctctcg
     6001 ctcggtgcgc aaaatcaccg gacggttatc gtaaagggta tcatccaggt caaaggtgag
     6061 cgccgagatg cgccccaaag gccggtaaaa acgcattatt tcccccgttt ggcgcgtgga
     6121 tgcgccgcat cgtacaccga ggcaaggtgt tgaaaatcaa gatgagtata gatttgcgtg
     6181 gtggagaggt tggcatgacc cagtagctcc tgcacaccac gaagatcgcc gctcgactcc
     6241 agcatatgcg tagcgaacga gtgacgtaat ttatgtgggt gaacgtgatt attcagccct
     6301 tgttttatgc cccattcggc aaagcgtttc tgcacattac gcgcggagat acgcttgccc
     6361 agtttcgaca gaaaaagcgc gtcgtcttcg ctaccaaaca ggtcgcgcaa atcaagccag
     6421 tgctcaatcc acgccacagc gttgcgacca atcggcaggc ggcgctcttt gctgcctttc
     6481 cccataaccc acacttcacc agactccagg tcgaggtgtt taatatccag ccccaccagc
     6541 tcagaaagac gcagacccgc gccgtacatc acttccagca ttgcacggtc gcgtacagcg
     6601 aggggatcgt tgatatcaat atccagcagc cgattcatat cgtcgacgtc gatgtttttc
     6661 ggcagatgac gcggcgcttt cggtgccgaa acacctttcg ccgggttagc tttgagttcg
     6721 ttctggctga ccagccagtc aaaaaagcta cgtagcgcag aaagccgtaa cgccagactt
     6781 gctgctccca gccctttacg gcgactgcgt acagcaaaat tgcgcaccat cgtcacatca
     6841 cattgctgcc agctttgcag gccgttttcg ctggcaaaat tgatgatcgc ctcaagctga
     6901 cgctggtagt taagcagggt tatcgggcta agctggcgct ccacgctcag ataacgtagg
     6961 tagcgttcta catcggtgtg taaatcggtc atacgcgttc aatccaacgc tccagaagct
     7021 ccggcaacat cagcgcaatt tcatgaagta actgcgttcc ttgcccttgt tgatagtgac
     7081 tggcatcgcg actggtaaac agcacgacac ccaaatcagc atcgcttccc agcatcgaca
     7141 tcgccaccga tcccaccgct ttcgcttccg gtagcaccac cagcagctct ggtccgttaa
     7201 gcggcccaag atagtgctgt tcctgcccca aacgctgaat acgcagcggt tcgaaagact
     7261 gacggcttaa tgccagatga gtgtggttcg acggcgcacc taagcgccag cgatccggaa
     7321 acaggcgcag actcgcacct gccaggccga gatcgcgcgc ccagcggtga aagcgcatca
     7381 gcatatcgtc gagactgctg gcggcggtga gactgcgctg caggtagagt aggcgataaa
     7441 acaggccttc gttggcgata gcctgttcca tcaacagcgc catgttctct tccagaacat
     7501 gaatatgatt acgtgcgcgg gccatgtgcc actcgaccaa cgaaacggtg ccgcgtaccg
     7561 gatgcggcac acgtatcgct tctactgcgc gcgcattacg gataaaaaac tcaggatttt
     7621 taatcagata atcgacaacc gcccggtcat caagctccgt gagtgtttcc tgcagttctt
     7681 cccctggttg cttcatagat gaataaatcc gtcgtagaca tgtaccgccg ggccagtcat
     7741 atataacggg tgacccggac ctttccaggc gatatcaaga cgaccgccgg ggagttccac
     7801 gcgtacttct tcggccagca aaccttgctg aatccctact gcaaccgccg cacacgcgcc
     7861 gctgccgcag gcctgggttt ctcctgcccc acgctcataa acgcgtaaac gaatatgctc
     7921 gcgcttaacc acttgcataa aaccgatatt ggcgcgctcc ggaaaacgct cgtggctttc
     7981 cagaacagga ccaagcgttt ctaccgccgc ggtatcgaca tcatcgacct gaatcacgca
     8041 atgcggattt cccatcgaca ccacgccgca taagattgtc tgctcggcgg cgcgcataat
     8101 ataggtcttt tccgctttgt tagcgcgaaa cggcacggcg gaaggttcga agttgggttc
     8161 gcccatattt acgcggacca gatcatcatc ggtgacggtc agaaccatcc gcccgttggc
     8221 ggtgctgacg cggatatcac gcttattggt cagtcctttc agacgcacaa aacgggcaaa
     8281 gcagcgcgca ccgttgccgc actgcgccac ttcactgcca tcagcattga aaatgcgata
     8341 gtgaaaatcc agttcaggat catacggcgg ctcaaccacc agcagttggt caaaccctac
     8401 ccccaggtgc cgatcagcca ggcgacgaat cagctccggt gaaaaaaaga cattctgcgt
     8461 taccgcgtcg acgaccataa aatcgttgcc aaggccatgc attttcgaga actgcatcat
     8521 ttactccaat cacgcgggta cagaaactga cttttagtaa ttcacctggg atggaccatc
     8581 gccagtggcg cgatcgtttt tatccggcac cgtggattgc gtttgcgtct ctaccggttt
     8641 ggtcggcggc ggtgcgtttt tatctgcagg cgggaaatag agcggacctt tcagaccgca
     8701 gcccgtcagg ctgaagagag taattaatac agtgagtgcc ttaaacacgt ttttcattat
     8761 ggattgcctg taaagttcat cgctttctgc tttctatcat cgcaggagaa gacgtaaaag
     8821 caagcgttta gcgactcttt atgaagtctg aaaagtgagg aaatgctgcg agtataacgc
     8881 ttactcgtgg ggatttatgt tttgctggta gtttcgtgat cggcacctcg cgccaggatg
     8941 gctctatgca ctatcgcaat gtagtttgtt tgttggcttg ttcgttgttc ttatcgtcgg
     9001 catggagctt ttaaaagagt ggcgcaccat gcaattttgt tgtctgacct tagtcgccag
     9061 tgacatacaa ctttgccaac ctggtgataa gagggaataa aatctgatgg ggctatttac
     9121 ccaactggaa aaatttgacc aaaagcttac gcgcggttat gcccggtggg ggcgctgggt
     9181 atggcggacg ttaattgtcg ttccggtact ggtcgtgttg tggaatatag gacaggcagt
     9241 atggggagga ccaagaggtg gagtgattct ggaaatccac agtgaaattg accgcccgat
     9301 actgggattc agtgttaacg gcgtggcggg cgcgaatgct tttgcaaatg gtggcggttc
     9361 aacaacctgt tgtggtgatg ttagtgggga tacggcagag gttatctgga cattaagtac
     9421 tactcgtaca cagtataatg ctggaatgcg tctggagaaa cggaatatga cgttacctct
     9481 ccccaaaaga gaatggggag aagattttct gcatgtgcat tttatgcctg gcgataaagt
     9541 gctgctgggg tggagtaaag attctttttc tccgtatgaa gatcttcaca acggggggta
     9601 caagacccgc gttcggcagg atgtgaaaga taaactttat ggtacaggga aaatgaattg
     9661 atagatttct ataaaaatca cgttcggcag aaagcgtaat aatgtgggga atgttagata
     9721 ccggtggtgg gacctaagta caacacgcga aagacaataa agtaggtaac ggtgggcaga
     9781 gtcgcatgtt gacacccttc ccggcgtcaa tccggcacat tttaacgtga tcagtcacac
     9841 catggcgcag tagctcgtat ttatatcgtg aatgaaaacc tcaagtttac cccagcaact
     9901 atcagaaaat agcgatggcc tttatagatt ctgaaaagtg acgatttgcc gcaaaagtta
     9961 cgcttacgtg cgaggaatta tgttctgctg gtagtttcgt gatcggcacc tcgcgccagg
    10021 atggctttat gcactatcgc aatgtggttt gtttgttgtc ttgttcattg tttttatcgt
    10081 cggcatgggg atgtcggctt gatccgcctg aacataatat ttaccagaaa cagggaaaag
    10141 gcgtggtata cctgcgccct tataaagaaa caaatctttc tcttcctgac gtgaattaca
    10201 aaagtttgcg ccgtttaccc aatttattaa tagacccgac aacactagac aaatgggata
    10261 aagtacctcc tctgactgat ctgacaaccg actacctcta tgaaggcgca caggcctggt
    10321 atcctcatta ctcctggcat agcgatggtc gctatatcct ctacgcgggt gaagttgtgc
    10381 aaaacccgcc agacaaacct cccgtcgatg tcgcctcgtt caaggcgtgg ggtgattttg
    10441 ccgcagataa acacagtctc tatttcgaag gcaagcgcac cgataacaat ggcgggggaa
    10501 atcgcttaga tatcaaaaca ctgcatcagg tggaattccg gcctccctgg gatcctgact
    10561 tactggggct gatattacgt gacgctaatt tcttgtatat caatgggcat cgcctcgcag
    10621 atcccgatag tttccgtgtg ctggcacaaa agtcatggga tcagcgagga aagttttcta
    10681 ccgcgtttaa tccctgtgtg gctatgccat ttggcccctg ggataccctg gctcgtacac
    10741 ggaccaaaat cctgatcaac ggcgaacagc ttgatgccga cccggacacc ttttccgtcg
    10801 tgcgctggat gcccggctca ctcttgacct ggcgtgataa aaacgggctg cagcgtaaag
    10861 tcctcgacaa ggaaaatctg gagtgggatg aagatttaac aaagcactgt ctggattttt
    10921 ctctgctgga aaagaaagtg ttctggcgta aaggacctgc ttgtaaacag gaagaattac
    10981 ccggactcga tccggagcag tttcaaccca tcagtgatgc tgtcgcccag tatcaggact
    11041 cgctttatac catcatcgaa acagagtctg gtgaccgcaa gctggaaatc gtgaaacttg
    11101 atgatcccaa tcttattatc aacaaacgtt tcaacgccgg gaaacgccac ggctatttac
    11161 ttacgcgtgc cgaagggtgg ccataccatt ccggtttaca tgtgtttgaa tctgacggac
    11221 cgctgatctt actggataac cgttctccgg atgaacgcga agcccatctt aatgaccatc
    11281 cctttttgcg cagatggtat gcccgcgata accgctacat ttacagcttt gatggcgcgc
    11341 agctctggcg ataccgcacc gctgatccga aacaagttcg cttaatctgg aaggaacaac
    11401 attcgggata tggctatggc gtaaattaca aaacgggata tctggacgga aaaattactg
    11461 atgacggcga atttattcct gccccgcgca atgaggcgac aaaatgaagg gtcaaataat
    11521 cacagttgca ctcatcctgt tgggacacac atttctgacg cctgctgtac aggccatagg
    11581 gttcgactat tacaatgatc acggtgttat gtcttacggt aaaggttatt ggggggataa
    11641 gaaaatcatt gcccagtttc cggagatgag cagggccgat ttgcgccttg tgacaaatat
    11701 atccggggag cgggagttgg tggaaggcta tatacccacc gatgaaataa gcataaaaaa
    11761 tgaggaatac cactgggtga cagacggccg cgttattctc tggcgtggca aaatcgttag
    11821 caatccaccg ggaacaccca ctgtcgatat tgccagcttt caggctatgg gccgcttcgc
    11881 ggtcgataaa tatagcctct attttgacgg acagcgcact gaaagtaata gcggcgcgtc
    11941 acgtgttgat ctggcgacac taaaagctat cgaaggtaac tctaccacgc tggtggatag
    12001 taaaaatctc tatttgtccg gtcgacgtca ggggagcagc agtaatgtta ctgtattaga
    12061 aaaaagatgg tggggtatta atccacgtct tatgagtgta aacagaaatt tgtattccaa
    12121 tgatctgctt atccgcagtg ggcagaatat ttatttaaat ggcgttcacc ttacggcgaa
    12181 tgcagactca tttgagataa ttcgctggat acctcattca ctgctggttt ttcgcgacaa
    12241 taagggtatg catcgttatc cctttgggca attgtctggc aaagcgatac ccgtagatga
    12301 tgacgtctct tttgaagtag gggaaagtcg cgttcgctgg cgtaaacagc tcacgtccga
    12361 ccgtcagtgg agcaagtgga tagacctacc agatattgaa cctgaacaat tccatctgat
    12421 tactggcaat attgcgcaat ataaagatcg gctgtatgta acaaaattat cgacatttgg
    12481 tgaagaccag cttgagataa tcccgctgga tacgccagac ctggtcattg atcgctcatt
    12541 taatagcggc aaacagcatg cttattttat ccgccaatta cggtcaaaga gcgtgcaaat
    12601 tattccagtt aacggtccgc taactaaaaa cgatcgcttc gcttatgacg atcgcaatgt
    12661 ttatacatgg accgatacag aggtaaggat tacgccctcc ccctgcccgg cgaaaacaca
    12721 cgtcagagaa gagaatgtgc gtgaacttca caacagagac attattattc cgttgacgga
    12781 tgactcatgc cggaacgcag caacagacgg gcaaactttg aagccctgac cccataccgc
    12841 tatactgccg ccatcactta aagacaggat acaacaatga acgacagtga atttcatcgc
    12901 ctggctgatc aactctggct gaccattgaa gaacgcctgg acgactggga tggcgacagc
    12961 gatatcgact gcgaaatcaa cggcggcgta ctgaccatta cctttgaaaa cggcagcaaa
    13021 atcattatta accgccagga gccgctgcac caggtgtggc tggcaaccaa acagggcggc
    13081 taccattttg acctaaaagg cgatgaatgg atttgcgatc gcagcggcga aaccttctgg
    13141 gatttgctgg aacaggcggc gacacagcaa gcgggtgaaa cagtcagttt ccgctaagat
    13201 tgcatgccgg ataagcctcg ctttccggca cgctcatcac gaaaaatact gctgtaatag
    13261 cggcgtatcg ttgtcctgat tggcgggcgg catgttaccg atagattttg tgcggaacgg
    13321 aatcacctgt tcacgaccat caaccttcac aatctgatag aactgcggca ggttgaagtt
    13381 gataaagctt gagccgtagg tgaagcgatc gtgcgatgac gagtagaagc gactgacgtc
    13441 acgtaccagc tcctctttgc tgccttcgca gtggtgatac acctcaaccc ggttgctttc
    13501 gtcgagaatg tagatattaa agccattctc gtcttgcgtt tcttcgaaga agaactggat
    13561 gatcccttcg ctggcaaagc cgtccaccac cgccggtaat ttgacgtgat tggtttcaac
    13621 ctgcactgac aagccgtgca gtttgttatg cgaaatagcg ccataaaact cgatggcgtt
    13681 ttccagtttt tgtaccgata cattcaggcg ttcgaagaac aacccccagg tttgaccaga
    13741 aacgcgcagc gccttgaaac gcccggtttc ctggcgggtg ctggaaaggc gcagttcaat
    13801 acactcagaa accagttgct gcacgcgagt acggattaac ccgcgcagat gctggctata
    13861 acagaagact tccacgctat ctggcggtgc ggcgtcctga tgcattttgc cgagaatagt
    13921 tttcagggct tcgatcatcg attgctcgcc gttgaagtgc agcgtacgca cttcgttcca
    13981 cgagttgcgg tacagcaggt caacgctacc taccaggcaa ttttgattct cgccaaagct
    14041 gaagacatcc agcttacgga aatcaaaatg caccacctga ttgcggaacg ccgctgtcgg
    14101 gtcatattcc aggttaacga taatcgccag atggcggatc tcacacgggc tgtagagcgc
    14161 cttcggtgtc ggtgcaggta agcgcagcgg gaaatggtgc gacacgtcgg cgaccatctc
    14221 ctgcaactta ggcaaatcga caatgccgtt acctttaata tacaaacggg tgcgcgaggt
    14281 cagcaggccg ttaaaccatg cccacgccac cagtttgttc aggtaacggt tatattccag
    14341 cggctgatgg ctgatgatcg actcaatatt tggcgcgcgg ttatacagat accaacctga
    14401 acggttagca cggcccggcg gcacataaat aaaggtcaga ttcggttccg agagatcggg
    14461 tgaaatctgc gggtttacca gcgtcacttt acctggtaat gcttcaaacg cggcatacag
    14521 cttacgcgtc agcacgccga tatcctgcgg actggcggag acgctaaggt tattgcgacg
    14581 cgcaaagcgg atcagattac ggtagctctg catcatcgcg tcgagcaact cgttgtgcgc
    14641 ctcacgcacc tgatcaatct tccagttagc gcggttatcg agcattgcca gacgagcttc
    14701 gtcccaaccc cactcgctca ctaactggct caacactgcg cgacgccagc ctacgcaggc
    14761 gcgttcacgg ctgagctttt cgcacacttt taaatagaag cagcgacgta ctaaatccag
    14821 acgggtaaaa tcttcaatcg ccgtcaggta ttcagtaaca cgctccagca tcatgcagta
    14881 tggatcgaga ccaaacgata caatctcgcc gtcgtgcaaa cgctgtttga tatctttcgc
    14941 cagcagacgt gggttcgggt attcccagga ataggcttcc agcagcagtg ttttcagtac
    15001 cgctttgtat ggggaatcga tactcttgta gagctgccaa aggctggcac caaagtactc
    15061 ttcagcagaa agcgagctta agccaccgag atccagccat tcatttggcg tcagcacgcc
    15121 ctgcgcgtaa agcgtcatca catagtcgtc gtaatgctct tcttcgtcgc acggcaccat
    15181 attccacaga atacgcttac cggcgagacg cacggcggta cgataaaatt cgtcaagcag
    15241 cagtatatgc tgggtggagc cacaatcttc gccccccagg ctgccgcttt cattatgacg
    15301 gaagcggttt tcatcaatca ggaagaagct gacttccaca cccagcgagg cggcccagct
    15361 ttccagcaga ctacatttac gttgtagtaa ctggcgctct tcgctatcga gccaggattg
    15421 atgacagacc cagatatcca ggtcagagga acaactttgc cctacggacg aggtgctgcc
    15481 catggtgtat acaccagtaa ttggaagctc acctttcggt gggtcctgta ctgacattcc
    15541 acgatacagt tcaagctcgt tcaggtagtg gcgttgagtt tcatcaggcg tgtaaaggca
    15601 aatgcctttg ggaacgttac catcaaggta acccggcatt agcggatggt gatagtgcaa
    15661 caatgtcggc agtagactgt agacctgttg gaatgcaggc cccatagcag caagcgcgcg
    15721 atccacacgc aattgattta tggcatccag tctctgtttc agagtctcaa tatagaggta
    15781 caagacgtat cgcctgattt gctacccgtc atgactgtga ttccgccaac atcaacggta
    15841 acacgcggca ttcgggatat ttcgtatgtc aaaggtaacc gttaccactt ttcgcgcctg
    15901 gtttttttag tttcacgacg aaaaaatggt ctaaaacgtg atcaatttaa caccttgctg
    15961 attgaccgta aagaaagatg cgctacatac aagtgtagca ccgtttattc tctgtaaatt
    16021 ccttattacg acggcgtgaa acgcctgtca ggatccactg ccagacctca ttttacggtt
    16081 tgcgcaggcg tctacgtttc accacaacac tgacatcact ctggcaagga tgttaggatg
    16141 gaccacggat gataatgacg gtaacaagca tgttagacaa tgttttaaga attgccacac
    16201 gccaaagccc acttgcactc tggcaggcac actatgtcaa agacaagttg atggcgagcc
    16261 atccgggcct ggtggttgaa ctggtaccga tggtgacgcg cggcgatgtg attcttgata
    16321 cgccgctggc gaaagtaggc ggaaaaggct tatttgtaaa agagctggaa gtcgcgctcc
    16381 tggagaatcg cgccgatatc gccgtacatt caatgaaaga tgtgccggtt gaattcccgc
    16441 aaggtctggg gttggtcact atttgtgagc gtgaagatcc tcgcgatgcc tttgtgtcca
    16501 ataattatga cagtctggat gcgttaccgg caggcagtat cgtcgggacg tccagtttac
    16561 gtcgccagtg ccaactggct gaacgccgcc cggatctgat tatccgctcc ctgcgcggca
    16621 acgtcggcac tcgcctgagc aaactagata acggcgaata cgatgccatc attcttgccg
    16681 tagccggact aaaacgttta ggtctggagt cccgcattcg cgccgcgtta ccacccgaga
    16741 tttctcttcc ggcggtagga caaggtgcag tcggtattga atgccgcctt gatgatacgc
    16801 gcactcgcga gctgcttgcc gcgctgaatc accacgaaac tgcactgcgc gttaccgcag
    16861 aacgcgccat gaatacccgt ctcgaaggtg gatgtcaggt gccaattggt agctacgccg
    16921 agcttattga tggtgaaatc tggctgcgtg cgctggtcgg cgcgccggac ggttcgcaga
    16981 ttattcgcgg tgaacgccgc ggtgcgccgc aaaatgccga acaaatgggg atttcgctgg
    17041 cagaagagct actgaataac ggcgcgcgcg agatcctcgc tgaagtctat aacggagacg
    17101 ccccggcatg agtatcctgg tcacccgccc gtctcccgct ggagaagagt tagtgagccg
    17161 tctgcgcaca ctggggcagg tggcctggca ttttccgctg attgagtttt ctccgggtcg
    17221 acaattaccg caacttgctg atcaactggc tgcgctgggg gagagcgatc tgttgtttgc
    17281 cctctcgcaa cacgcggttg cttttgccca atcacagctg catcagcaag atcgtaaatg
    17341 gccccgacta cctgattatt tcgccattgg acgcaccacc gcactggcgc tacataccgt
    17401 aagcggacag aagattctct acccgcagga tcgggaaatc agcgaagtct tgctacaatt
    17461 acctgaatta caaaatattg cgggcaaacg tgcgctgata ttacgtggca atggcggtcg
    17521 tgagctaatt ggggataccc tgacagcgcg cggtgctgag gtcacttttt gtgaatgtta
    17581 tcaacgatgc gcaatccatt acgatggtgc agaagaagcg atgcgctggc aatcccgcga
    17641 ggtgacgatg gtcgttgtta ccagcggtga aatgttgcag caactctggt cgctgatccc
    17701 acaatggtat cgtgagcact ggttactaca ctgtcgacta ttggtcgtca gtgagcgttt
    17761 ggcgaaactc gcccgggaac tgggctggca agacattaag gtcgccgata acgctgacaa
    17821 cgatgcgctt ttacgggcat tacaataact ctcataacag gaagccataa tgacggaaca
    17881 agaaaaaacc tccgccgtgg ttgaagagac cagggaggcc gtggacacca cgtcacaacc
    17941 tgtcgcaaca gaaaaaaaga gtaagaacaa tactgcattg attctcagcg cggtggctat
    18001 cgctattgct ctggcggcgg gtgtcggttt gtatggctgg ggtaaacaac aggccgtcaa
    18061 tcagaccgcc accagcgatg ccctggctaa ccaactgacg gcattgcaaa aagcccagga
    18121 gagccaaaaa gccgagctgg aaggcattat taagcaacaa gctgcacagc ttaagcaggc
    18181 gaatcgtcag caagaaacgc tggcaaaaca gttggatgaa gtccaacaaa aggtcgccac
    18241 catttccggc agcgatgcta aaacctggct gctggctcag gccgattttc tggtgaaact
    18301 cgccggacgg aagctgtgga gcgatcagga cgtcacgacc gctgcagcgt tgctgaaaag
    18361 tgcagatgcc agcctggcgg atatgaatga cccgagtctg attaccgttc gtcgggcaat
    18421 taccgatgat atcgccagcc tttctgcagt ttcgcaggtg gattatgacg gcatcatcct
    18481 taagcttaat cagctttcaa atcaggtaga taacctgcgt ctggccgata atgacagcga
    18541 tggttcgccg atggattccg acggtgaaga gctttccagt tccatcagcg aatggcgtat
    18601 caatctgcaa aaaagctggc agaactttat ggacaacttc attacgattc gccgtcgtga
    18661 tgacaccgcc gtaccgctgt tagcgccaaa tcaggatatc tatctgcgcg aaaatattcg
    18721 ctctcgcctg ctggtcgcag cacaagctgt accgcgtcac caggaagaga cttatcgcca
    18781 ggcgctggag aacgtctcca cctgggtacg agcttactac gatactgatg atgccaccac
    18841 caaagcgttc ctcgacgagg tggaccagtt aagccagcaa aatatctcga tggatcttcc
    18901 ggaaaccctg caaagccagg cgatgctgga aaaactgatg cagacccgcg tgcgtaacct
    18961 gctggcacaa ccggcagcgg ggacaacgga agctaaacct gcacctgcac ctgcacctgc
    19021 acctgcacct gcacctgcac ctgcacctgc acctgcaccg caaggtgata ctccggcagc
    19081 cgcgccgcaa ggagaataac catgctaaaa gtgttattac tctttgtttt gctgattgcg
    19141 gggatagtgg ttggcccgat gattgccggt catcagggtt acgtgctgat ccagactgac
    19201 aactacaata tcgaaaccag cgtcacgggc ctggcgatta ttttgattct ggcgatggta
    19261 gtgctgtttg ccattgagtg gctactgcgg cggatcttcc gcacaggcgc acacacccgt
    19321 gggtggtttg tcggacgtaa gcgtcgccgt gcccgtaagc agaccgaaca ggcgctgctg
    19381 aaactggcgg aaggcgatta tcagcaagtt gaaaagctga tggcgaaaaa tgccgatcac
    19441 gcggaacaac cggtggtgaa ctatctactg gctgccgaag ccgcgcaaca acgtggtgat
    19501 gaagcacgcg ccaaccaaca tctggaacgc gcagcagagc tggccggcaa tgacaccatt
    19561 ccggtagaaa tcacccgtgt acgtctgcaa ctggcccgta atgaaaacca tgctgcacgc
    19621 cacggcgtgg ataagctgct ggaagttacg ccacgccatc cggaagtatt acgtctggcg
    19681 gaacaggcgt atatccgcac aggtgcatgg agttcgctgc tggatatcat cccatcaatg
    19741 gcgaaagccc atgttggtga tgaagaacat cgtgcaatgc tggaacaaca ggcatggatt
    19801 ggcctgatgg atcaggcgcg tgccgataac ggtagtgaag gtttgcgtaa ctggtggaaa
    19861 aaccaaagcc ggaaaacgcg tcatcaggta gcgttgcagg tggcaatggc ggaacatctt
    19921 attgaatgtg acgatcatga tactgcccag caaattatca tcgatggcct gaaacgccag
    19981 tacgacgatc gcctactgct gccgattcct cgactgaaaa ccaacaatcc ggaacagctg
    20041 gaaaaagtgc tgcgccagca gatcaaaaac gtcggcgatc gcccgctgtt gtggagcaca
    20101 ctgggccagt cactgatgaa gcacggagaa tggcaggaag catcgctcgc cttccgcgcg
    20161 gcgctgaaac aacgtccgga cgcctacgat tacgcatggc ttgccgacgc gctggacaga
    20221 ctgcacaagc cggaagaagc tgcagcgatg cgtcgcgacg gtttgatgtt aacgttgcag
    20281 aacaacccac cacagtagtt ccttctcacc cggaggcaag cacctccggg acctttctga
    20341 tgcataaaaa aacgcctgct cttattacgg agcaggcgtt aaaacaggtc tgtatgacaa
    20401 caagtgggtg cttcactcaa cgttgtgtcc atggtgtctg atgaggcata agcgacatct
    20461 gtcagtggac gataagcacc gtaaacggct ctgcgtcatt ccggagttta tgaggcacta
    20521 aggcgaacat aagagatgga atgagcatct acccgtttat tatgccacag agaatcggga
    20581 aataacatcc cttaacactt gttatgagat aattctgtaa tcctctttgc ttcctgagta
    20641 ataacttcct gagtgaatat ttaacctgag cttgatccta cacatactta ttatgaatga
    20701 taaaattcat tcaattaata acacatatat taattgccgt taaaactaaa aaaagcatca
    20761 ataatcaacg cgatataata aacctgcctt acatatcaac tgcgccagag gtaggattga
    20821 aaacgctctc ctgattttcc aattcatttt ctggataaat aaataattta tttttgtcac
    20881 tattatttat gtaatcatcc tgtcagggag agggatctca attatcaatg cttaattacg
    20941 tcatcatttt gatgacatga aaccggagca ttttgcatgg aattttcgtt ttcacccaaa
    21001 cttcttgttg ttgctgtcgc cgccgctctt cctctcatgg ccagcgcagc agataccccg
    21061 tcaactgcca ccgcacgcaa aggctttgcc ggatacgatc acccaaacca gtatccacag
    21121 aacgctgcaa tttattgaat ttgcacgatc atgtaggccg gataaggcgt ttacgccgca
    21181 tccggcaatc aaccgcaggc ggccacaaaa tggccgcttt tttacttact caccaccagc
    21241 aaatgcgcat gcataatgtc actggccggg cgaccgtgcg ccagcaaatc agccattgct
    21301 ttaagatatg gcggtagatg gcggaaataa cgctgatacc cggcacacaa ataattcagc
    21361 cccggtttgc cgctggcatc gagcatgaag cggtgtttcg ggcagcctcc ccagcacgct
    21421 tttaacacgt tacaactgcg acactgcgcc ggtaattgct taaatttatc ttcaccaaac
    21481 acctgctgtt gcggggaatc gatcatttct gcaattgttt gctgatgcat attccccagc
    21541 cgatattgcg gataaacata gtgatcgcag gcgtaaacgt cgccgttgtg ctcaacaatc
    21601 accgagcgcc cacaggttgg ctgatgatgg caaaccgcac ccggcgcacc gacaaaattg
    21661 gcaaacgccc attcgatatt catcacgaaa atcttgccga cgtcgcgttt gatccagtgg
    21721 tcgaatatcg ccaccagaaa ttcaccaaat tcatcggggc gcaccgacca ttccgtcagt
    21781 tccccctgaa tatcaccagg cgcatgtagt ttcagtccat cgctggcagc tgtttcatca
    21841 gccaggcgct cgaccaccgg aataaactgg atgaattcga ctcccgcatc gcacaaaaaa
    21901 tcataaacct gcaacggttg ctgcgcgctg gtgcggttga cgcagaccag cacgttatag
    21961 tcgacatgat gtttttgcag cagcgtcagg gcacgcatca ccagcttatg cgttggtctg
    22021 ccacctttgg tcacgcgata ttgattgtgg atctcagccg gaccgtccag cgataaccca
    22081 acaagaaaat gattttctgc cagaaatgca caccatttat catcgagcag cacgccgtta
    22141 gtctggaagc tgttacttat cttcctgcca gcaccgtatt tcgcctgtaa ctttacggca
    22201 cggcggtaaa aatccagccc gagtaacgtt ggttcgccgc cctgccaggt aaaagccact
    22261 tcgttttgcg gttcgctggc agcgatatag tgacggacat acgcttccag cgtgtcatcg
    22321 tccatatgcg tgactggctt ttcgcggtaa agggattgtt tttcgagata aaaacagtag
    22381 tcacagttca gattacaatc ggaaccactc ggtttcgcca tcacatgaaa agcacgcgtt
    22441 ggaacctgtt gcagcatggt cactccttgc ctgaagtcat ccttcaatac gttaagggcg
    22501 aaatcatacc gtggggcaat gagttattac gggcgtttat tgcgaaggga gggtgacgaa
    22561 atgcacagaa aacaaaaaac cccgccgaag cggggttcaa aattggtcgg cgagagagga
    22621 ttcgaacctc cgacccactg gtcccaaacc agttgcgcta ccaagctgcg ctactcgccg
    22681 aaatactgct ttttgaattt ttagttcaat tctttaaagt cgtggtgcga ggggggggac
    22741 ttgaaccccc acgtccgtaa ggacactaac acctgaagct agcgcgtcta ccaattccgc
    22801 caccttcgca ttgtcacaac ttctaataat ggggtggcta atgggattcg aacccacgac
    22861 aactggaatc acaatccagg gctctaccaa ctgagctata gccaccacta caaatcttgt
    22921 tacgcggtat tactaccacc gcagctcaag cgccgggact aaatggcgcg cccgacagga
    22981 ttcgaacctg agacctctgc ctccggaggg cagcgctcta tccagctgag ctacgggcgc
    23041 ttagcgccgt tgcgggggtg gataatacgg acttcccacc cctctgtcta gtcccttttt
    23101 aaataaattg cgcgtttggt tacggtttgt gcgttttgct gattattcct ccagtttatg
    23161 cgctttcccg tggcgattaa ggccaaaaac tttataaatc gccgtcaccg ccagcataaa
    23221 gatgatgcca acaaacagcg acatacgcgt atcttcatta aagtacatgc cgatcaaaac
    23281 gcaaatcagg aatgccattg ttacgtagtt ggcccacggg aacaggattg agcggaacgg
    23341 atggctggca atcgccgctt tatgtgcacg ccgaaaacgc agctggctta tcaatatcac
    23401 aaaccatggc accatccccg gaagcacgct ggcactgtag acgtagacaa acacacgctg
    23461 cggattggga atgatgtagt tcaggcatga gccaattagc agaatagcaa tagatactgc
    23521 cacacccgca actggtacgc cgtgacggga aactttcgcc atcgccgccg gtaactgacg
    23581 gtttttcgcc agtgcgtaga gcatacgtcc gcaactgtac atgccgctgt tacagccaga
    23641 gagcgcagcc gtcagcacca caaagttgat aatgcccgcc gctgcggtaa taccgatttt
    23701 ggcaaaagtc agtacgaacg ggctgccgtt gctgccaatt tcattccacg ggaagatggt
    23761 gacgataacg aaaatcgcgc ctacgtagaa aatcaggatc cgccacagca ctttgcctac
    23821 ggcactgcgc agcgtcacct gcggattctt cgcttcaccg gcagtaatgc caatcagctc
    23881 cacgccctgg taggacgcca ccacaataca cagcgcggtc aggaaccctt tccagccacc
    23941 cgcaaagaaa ccgccatgct ctgtgagatt gctaaaacca atcgactgcc cgccattgcc
    24001 aaagccaaag aaaatcacgc ccaggccaat gacaatcatc acgataatcg tggtgacttt
    24061 gatcatcgcg aaccagaact cgatttcgcc gtacaaccgc accgccgcca gattcgccaa
    24121 cgccaccagc gccactgcga tcaatgcggg tatccactgc gccatctccg ggaaccagaa
    24181 ctggacataa acgccaatgg cggtgatttc tgagatcccc accgccatcc acataaacca
    24241 gtaagaccag gcggtgagat aaccaaagaa cgggctcata taacgatgcg cataaacggc
    24301 gaacgaaccg gtaaccggtt cgaggaacaa catttcgccc attgaacgca tgatgaaaaa
    24361 gacgaatagc ccggcgatga tataggccaa caatacggat ggcccggccc atttcaaggt
    24421 actggcggcc cccataaaca ggccgacgcc aatggtgccc cccagggcga tgagttcgat
    24481 atgtcgagct tccagcccac gctgtagctc tggtttgtta tctgccataa atcctcgtgt
    24541 tgtgtttgca tgctttccgg tgttaccggt tatcgttatg ggtacatcga gtgttgcgaa
    24601 tgttttcgta attcaggaga aatggcaaat aaagcattaa aaatttgaat gctttgtgta
    24661 ataaaaaagc agacaggcga cggagtgacc actccgtcgc tttacaaaga gaggaaaatc
    24721 agaggttgcc ggtgtagtgc cagcgtaaat aacgcagcaa acgaagctga cgcttaatgc
    24781 ggctcggctg cgaaagcagg cggtagagcc actccagtcc cagcgtttgc cagattttcg
    24841 gtgcgcgttt tacgtgaccg gtgaaaacat cgtaagtccc gccaacgccc atatacagcg
    24901 catctggatg caccagacgg cagtcgcgca tgaagatctc ctgctttggc gatcccatcg
    24961 caacggtgac gatttgcgct ccgctggcat gaatgcgttc aaacagcgcc tgacgctgct
    25021 cgggtttaaa ataaccatcc tgactgccaa cgatattcac attccactgg ttgcgcagtt
    25081 tagcttcagt ttgcgccagc acttcaggtt taccgcccac aagaaatacc ggcgtccctt
    25141 ctttgcctgc gcgcgccatc agctcttccc agagatcagc accggcaacg cgggaaacct
    25201 gcgcctgcgg gtacttttta cgtactgaac gtacaacgct gatgccatcc gcatatttaa
    25261 attcggcagc gttaattagc tccctgacct cagcattatc ttcaatggtc agcatttttt
    25321 cggcattaat ggcaaccagc gttccctgct taagctgccc gtcagcaaac agataatcga
    25381 gggcgtgctg catatcacgc caaccaatca actgtaagcc acgcagcgta taggtcggtg
    25441 ccatggtgtt gttattcatt gttatccttc aacctgcgtc cggagcgatg attttgtacg
    25501 tttatgaatg agtccggcgc tttcaaaaag ccagtacaac agttttgcga tcatcagaca
    25561 tgcgccgaag accacgataa aaaagactac gcgtgagaca aacgaatcca gcccttcacg
    25621 tgccagcacg atcatattga agatcgcccc aaagcagaaa ctgtgcaata tcgcagcctt
    25681 atagcgatta ggctcgcggt tgcccagctc atacagccag tcgaaccatt tgatgatcag
    25741 accaaccacg atcgccccga gcgggatgaa caacgcgccg cccatcacca ccagtgagcc
    25801 tataagcgta ggcgagatcg ccagtccgga gtggttattc agcacttccc aggtaaagta
    25861 gttggctgag ttcagcacca tactcgggcg acccggccac agccaggaag ggataaagac
    25921 atagaaatcg cggacaattg gagccaggcc ctggaagtcg atgttgtcgt agttctgcaa
    25981 cagcaacgcc agattctccc acggcgagaa ggtgtcgcga gtgagataga gaaacgtata
    26041 gaacgcttca tcgccgctca cattcattcc atagcgtttt agcgccagcc agaacatgcc
    26101 cacaatcccc agcacgcccg ccgccgccag catccacaac gaaatccagc cgcgaataat
    26161 gccaataaac aggaagatag cgaatgcgat gatgatattg gcgcgagtgc cgccgacaat
    26221 catataagtc agcaagccaa aggcgaccgt gctgacgagg aaaaacagcc acgctttgct
    26281 atcctggcgc agaaagtaga ccaccagcat cgccgggatg aaaaagtaaa agaagcgttt
    26341 taacgccacg ccggagactt cactggagaa gatctgactg taggagttaa gccggaacag
    26401 caaaaagccg ttgtgcatga agaagatgcc gacgcttacc agcgcgatgc ccatcaggat
    26461 cacccacgta agattggtct ccacgcggtt catggtaaac agcggacggc gcggtacatc
    26521 agcaacgcgt ttgcgtaggc gggttttgta ggtgacatag taaaccgcgt agaagcagcc
    26581 cgcagaaagc aacgcctgca acaagatttc tggcggcgcg acaccaacat caaagcgaaa
    26641 taccagcacg ctggtcagcg ggaagccgaa gaaaaaggtg agcaaaaaca gcaatgaaaa
    26701 gaagacattg aagttaaagc gcacacggcg aaactcaaac caggtcagcg tggcaataaa
    26761 cagcgtgcag agcagccaga ccacaaacag gccactgaat tgcagcagac tcatgcgccc
    26821 tccccggcgg caatcgccaa cgcccgctgc cagccttgta gatagttagg gctaaagaag
    26881 gcaatggtgt ttttatccac cgacgccaac tggcgctgcg cttcacgcac aatatcctcg
    26941 ttgagatcgt cggtagtaaa cagcactggc aaatgctgtt ccgtcatatc ctgccagaac
    27001 ggattttccc ggttaagcac acaaggaatg cccgcctgaa tcagtaagca cagcgtacca
    27061 atgccctgct ggcgagcaaa aataaagtaa ccaagatcgc actgacgaag tagcgccaga
    27121 taggcgtcaa attccagttt ttcgctcaaa atttgcagat tttcttcgct gaataactcc
    27181 agccccgcct gacgaacttc ctcaatgtac gcttcgttat taggcggata tcccatcggc
    27241 accaccactt ttaccgtatc gccaaattgc tgatgaacgg cacgtaaagc ggcgacatgc
    27301 tcattgctga ggtcgccgga gttccccacc agaatggtca tttttccttc acgttgccga
    27361 tcgttcgcca tcgtattgag agaagggtcc atccgcgtcg ggaagtacag cagttctccc
    27421 cgcacctttg ggtgcgtttt ggcaaaaaag ctcaaatcgc cgcgggtggc aaatacacag
    27481 ccgactcgct tttgcgccag gcgacgtagt gggtaaaaaa gcttatatct caagccactg
    27541 gaaagctcat acaggtctgc cccccagata tgccagaaaa actggctggg cttaatgcca
    27601 ccactcagca gagccagcca cagtgtggga ttgaactgac cgtggaaaaa aaatcgctgc
    27661 tgacggttag cttttgcttt cgcgatgacc gcttccgcca gcgatttttt cccagggaaa
    27721 aattgcacag aaagcgccgg acagctatca cttaagccgt cgtccttgcc aacaaccata
    27781 aactcgcgcg cgtgctcgct cgtcgcagcc agcgcgtcat tgaaaaaccg caaaacggtt
    27841 cggttatggt gagggatatc cgatcccagt acgtgaatca gtacagtcat gcccgcctac
    27901 gccagagtaa aaacacgcca caacaaagag aaaaatagac gatataagtt gccatatatg
    27961 cctgcgccgc gcccagtgca ccatgcgcag ggattagcca gtgggcaaat accatcaata
    28021 aagtgaactg gctgatttcc gccagaatat aaaaccgcaa tgacgctttg gcgatcacca
    28081 gataaccaaa gacataagcg cccactttta acacatcacc cactaactgc caggcaaaga
    28141 gatcgcgcat agcggtaaat ttattcgaca acagcagcca gatagcaaaa tcacgcagca
    28201 gccagacggt aaaactcgcc gccgccactg ccggtaagac gaatttcagc gatttaacca
    28261 cttcccgggt gatatcgcgc ttttccgtta gccgcgacaa cgtgggcagc aaatatacgc
    28321 tgaacgatgc cgtaataaat tgcaggtagg catcggaaat actgctcacc ccttgccaga
    28381 tccccacttc atcccagcta tactgcgccg ccagcagttt acgcatcatg atgtaagcaa
    28441 caggcaaggt caccgacgta atcaacgcca tgagcgtaaa tttgctcaac tgccctgcca
    28501 gaccgttatc ccagctgggt ttcagatagc ttaacgggat gacaccacgt ttgatcaaca
    28561 tgatagtggc aggaattacc accagcgcgg gaatcagcgc cagacccagc aacgcccctt
    28621 cataaccgcc caaacggtaa ctgacgtagt acgcgagaac gccaatcaag ctgccgacaa
    28681 tcagcgataa cgcattacct gcggcatcgc gaaagccttt catcagcgcc agtaacaggt
    28741 tgccccaggc gatccccatt tgcaccagcg ccactaaacg caccagcccc tgatagtcgg
    28801 tattaccaaa caatccctgg ctgattggcg cagctgccag cacaaaaacc agcgccatca
    28861 gcgtagagaa accaagtacc atcgctgatg aagtgccgac cacgcggcgc agctgttgcg
    28921 gattatcatg gtactgggca acgtatttgg ttacaccgtt aaagatgcca gctccggcaa
    28981 gcacgccgag cacggtaatc aactggcgga aatttgccgc cagcccaagc cccgccggac
    29041 caaatgacac agccagcaac ttaccgacca gtaacccggc accaatcttg accagtgtac
    29101 tggccgccgt ccacaaggac gcttttgcca acgacatatc aggagaagta gttcaacaaa
    29161 gtcgcaatta ccgtacgctg attgacgggc gacaggttgt agaacagcgg caggcgcagc
    29221 aggcgctcgc tctctttggt ggtgtagcga tcttcaccgt ggaactcacc aaagcgttcc
    29281 cccgcagggc aaccgtgcag cggaatgtaa tggaacaccg ccatgatttc cgcttctttc
    29341 agaaagttaa tcaacgcgct ccggtcatca atatcccgca gtttaatgta gaacatatgc
    29401 gcgttctgca cgcagccatc gggaatcgac ggcagctcga tacgcccggc tttcgccaga
    29461 ggcgctaacg catcgtagta gttttgccac agcgccagac gttgctggtt gatacgatcc
    29521 gctgcttcca gttgcgccca caggtacgca gcttgcagat cggacatcaa atagctggag
    29581 ccaatatcgc gccaggtata tttatcgacc tgaccacgga agaactggct gcggttagtg
    29641 cccttttcac ggatgatctc ggctcgttcg attaacgctt tatcgttaat cagcgtcgcg
    29701 ccgccttcac cacccgccgt atagtttttg gtttcatgga agctaaagca gccaatatga
    29761 ccaatggttc ccagtgcacg ccctttgtaa gtggacatca cgccctgagc ggcatcttct
    29821 accacaaaca gattatgctt tttcgccaac gccataatgg tgtccatttc gcaggccaca
    29881 cccgcgtaat ggaccggcac gataacgcgc gttttgtcgg tgatcgccgc ttcaatcagc
    29941 gtttcgtcga tgttcatggt gtccgggcga acatccacaa aaacgatttt tgcgccacgc
    30001 agcacaaagg cattggcggt ggagacaaag gtgtagctcg gcatgatcac ttcatcgcca
    30061 ggctggatat cgagcagcag cgccgccatc tccagcgaag cggtgcagga cggcgtcagt
    30121 aacactttgg cgctgccaaa acgttgctcc agccactgct ggcagcgacg ggtaaaaccg
    30181 ccatcgccac acagtttgcc gctacccatt gccgactgca tatagtcgag ttcggttccc
    30241 accaccggcg gtgcgttaaa tggaatcatg tgatcacctg tataaccagt acgcggtgct
    30301 ttctacattc gcaccacttt gtatgtatcg tttaagcgcg gcggtgttgc ccatttgggt
    30361 cgccacccgc aaagttgttt taccgcgagc atacgcccag tttagcgccg tttgcatcag
    30421 ctcagcacct acaccgcgtc cagccagcag gccaattcgc gcatctgtcg cattgagttc
    30481 ccgtaaagag acataaccgc gaataccgcc ggacgccgca cgtaaaatca gacattgatg
    30541 atcaaaggtg ccgcgcacgg cattttcaat ccactgtgca taaaagcgtc tgctggcgtc
    30601 aggcgcatac cacggcgcac gaaaacggct ttgcgcaaat gcggcgctgg ctaactgacg
    30661 taatgcggga atatcggtct cttgtgccac tacagcacca ctatcactga cattgttcac
    30721 gggtagtgcc aaatcaactt caccttctac cagggagaat cccagctgtt gcagggcatc
    30781 cagttcaccc gtatttgatg ccgcaatttt ggcctgcacc cgtgaccacg gcgctaacac
    30841 gtctggcgtc aggagcggtg cttcagacgt aatgcgcacg atggcgctgt taacaccaaa
    30901 gaaggcgttt tcccaggtta gtggctcaat actggcgcgg acgggcacga agtaactcca
    30961 gcagatattg gccgtagcca gttttcgcta atgaactggc agcacgcttc acaccctcgt
    31021 catcgagcca gccgttacgc caggcgatct cttccaggca ggcaatctta aagccctggc
    31081 gtttttctac tgtctgcaca aaggtactgg cttcaatcag gctgtcgtga gtgccagtgt
    31141 ccagccaggc aaatccgcgc ccgagcagtt caacggtcag gttgcccgcc tcgaggtaca
    31201 tctggttgat ggaggtaatc tccagttcac cacgctccga cggcttcacc tgctttgcgt
    31261 actccacgac tttactgtcg tagaaataaa gcccggtcac cgcccagttt gacttcggct
    31321 gttttggctt ttcttccagc gagatagcgc ggaaattgtc gtcaaactcc accacgccaa
    31381 agcgttccgg gtccatcacc tgatagccaa ataccgtcgc cccttccgtg cgcgccgcaa
    31441 catgacgcag cttcggactg aagccctgac cgaagaagat gttatcgccc agcaccagac
    31501 aagaaggttc accgttgagg aaggtttcac cgatgataaa ggcctgcgcc agaccgtccg
    31561 ggctgggctg ttcggcgtat tccagctgga taccgaactc actaccatcg cccagcaggc
    31621 gctggaaata acctttatct tccggcgtag tgatgatgag aatttcgcgg ataccggcca
    31681 acatcagcac cgacagcgga tagtaaatca ttggcttatc gtaaatcggc aacagttgct
    31741 tcgatacgcc gcgcgtaatc ggatgcaatc gggtgccgga accgcccgcc aggataatac
    31801 ctttcatgtt tgcctccgct gaaaattagc ctttcagacc taaacgctcg ccctgatagc
    31861 tgccgtcctg cacctgcttc caccagcttt cattagccag ataccactgc accgttttac
    31921 gcattccact ttcaaaggtt tcctgtggca gccaaccaag ttcacgggca attttcgaag
    31981 catcaatggc ataacgcaga tcatgccccg gacggtcagc gacaaaggtg atcaagtcac
    32041 gatagtgcgc cacgccgtgc ggcttgttcg gagccagttc ttccagcagc tcgcaaatgg
    32101 tttccacaac atcgagattc ttacgctcgt tgtgaccacc aatattatag gtttcaccga
    32161 ctttcccggt ggtcgccacg caatacagcg cgcgggcgtg atcttccaca tacagccagt
    32221 cacggatttg ctgcccgttg ccatataccg gcagcggttt acccgccagc gcgttgagga
    32281 tcatcagcgg gatcagtttt tccggaaagt ggtaaggacc gtagttattc gagcagttgg
    32341 tgatcagcgt cggcagaccg taggtccgca gccaggcgcg caccagatgg tcgctactgg
    32401 cttttgacgc ggaataaggg ctgctcggcg catacggcgt ggtttcggtg aagaaatcat
    32461 ccgtcgagtg caggtcacca tatacttcgt cggtggagat atgatgaaaa cggaacgctg
    32521 attttttatc ttccgtcagc gtattccagt aagcccgcgc cgcttcaagc aatgtataag
    32581 tcccgacaat gttggtttca ataaacgctg ccgggccgtc aatagaacgg tcaacatggc
    32641 tttcggctgc cagatgcatg acacagtctg gctgatgctc agtgaatacg cgtgccagtt
    32701 ctgcccgatc gcagatatca actttctcaa aggcaaagtg ctcgctttgc gcgaccggtg
    32761 ccagcgacat caggtttccg gcgtaggtca gcttatcgac cactaccacc gcatcgctcg
    32821 tttcgttgat gatataacgc accagcgccg agccaataaa cccggcacca cctgttatca
    32881 gaatttttct catcagcgcc agactccttt ggcatcgacg acatactgct gatggacatt
    32941 gtcgccattg ataactttga actgactatg atcgaccagc atcaccagca cgtctgccgt
    33001 tgccagcgcc tcgtcaagct gcgccagagt acaaagcccg gtcagttttt tcggcaactg
    33061 gtggatgtta ggctcaacaa ccagcgtttc gccgctgtgc cactgggcga tcagttcagc
    33121 gatttccatc gccgggcttt cgcgcaggtc atcaatattc ggtttaaacg ccagaccaaa
    33181 gcaggcgatt ttcagttcac tggcgcgttt atcggtagca gcaaggcagt cggcaaccgc
    33241 cgctttcacc tgatcgataa cccagaacgg tttgtgatcg ttaacttcgc gcgcggtacg
    33301 gataagccgc gcctgctggg ggttctgtgc cacgataaac cacggatcaa cagcaatgca
    33361 gtgaccgccc acgccagggc caggctgaag aatattaacg cgagggtgac gattcgccag
    33421 gcgaatcagt tcccagacgt taatcccctg atcggcacaa atcagcgaca attcattagc
    33481 aaaagcgata ttcacatcgc ggaagctgtt ttcggtgagc ttacacattt ccgccgtccg
    33541 cgagttagtg acgacacact caccttcgag gaaaattttg tacagttcgc tggcgcgggt
    33601 cgaacaaacc ggcgtcatac caccaatcac gcgatcgttt ttaatcagct cgaccataac
    33661 ctggcccggt aacacgcgtt ccgggcagta agcaatgttg acgtccgcct gctcgcccac
    33721 ctgctgtggg aaagtgagat ccggacgcat ctctgctaac cattctgcca tcttctcggt
    33781 tgaccccacc ggcgaggtgg attcaaggat caccagcgcg ccttttttca gcactggcgc
    33841 aatggagcga gcagccgatt caacgtaggt catatctggc tcatgatcgc ctttaaacgg
    33901 cgtgggtaca gcaatcagcc aggcatccgc ttcaactggc gtcgtgctcg ctcgtaaaaa
    33961 accgccttct acggcagttt ttactacgct cgccaaatca ggctcgacga tatggatttc
    34021 gccacgattg atggtatcaa ccgcgtgttg gttgatatcg acaccaatca cctgtttttg
    34081 ccgtgaggca aacgctgctg ctgtaggcag cccgatgtaa cccagtccga taacagaaat
    34141 ggtcgcaaaa ctcatagtga tatccgatta ttttttaacg cttccagaat gcgagagcat
    34201 gcctgaccat caccatacgg gttatgggcg cggctcatag tttgatattc gttttcgtct
    34261 tttaaaagac gcgtcacttc ctcgacaatt cgctgcttat ccgtgcctac cagacgcacc
    34321 gtacccgccg tcaccgcttc cggacgctca gtggtatcgc gcatcaccag caccggtttc
    34381 cccagcgaag gcgcttcttc ctgaatgccg cctgagtcgg tcaaaatcag ccaggcgtgg
    34441 ttcatcagcc agacaaacgg taaatactcc tggggatcga tcagaatgac atttttcaca
    34501 tgccccagaa tgcgattgac cggctctctg acgttcgggt tgagatgcac cggatagaca
    34561 atctggatgt cctggtgcgt ggtggcgatg tctgccagcg cgtggcagat ttcttcaaag
    34621 ccacgaccga aactttcacg cctgtgaccg gtcaccagaa tcatcttttt atcggggtcg
    34681 ataaacgggt aatttgccgc cagttctgaa cgcagcgtgt cgctgctcat cacctgatca
    34741 cgcacccata acagtgcatc aatgactgta ttaccagtaa tgaagattcg gctatccgca
    34801 acgttttcac gcagcaagtt ttgccgggaa gtttcggttg gagagaagtg atacatcgcc
    34861 agatgcccgg tcaatgtacg gttagcctct tccggccacg gcgaatagag atcgcccgtg
    34921 cgtaaaccag cctcaacgtg accaacagga atacgctgat aaaacgccgc caggctggtt
    34981 gccagcgtcg ttgttgtgtc gccgtgaacc agcacgacgt ctggtttgaa ctcggcaaga
    35041 ataggtttta gcccttccag aatccgacag gttatctctg tcaggccctg tcctggctgc
    35101 attatgttga gatcgtagtc aggtacaatg gaaaagagtt tcagcacctg atcgagcatc
    35161 tcccgatgct gcgcagtgac gcaaacttta gcctcaaaaa aaggatcttt tgccaacgca
    35221 tgcaccaacg gagccatctt gatggcttcc gggcgcgtac caaatacagt cagtactttc
    35281 acatcgattc tcttcgaata agcggcgagc gcctttgcgc tcgccgcagc agtgttgcta
    35341 tttcgagcaa cggcgggtta atgcgacacc agccccgatc agccccccga caatgcccca
    35401 cataatcatc aggaaggcac gacgtgggct atcgcgtttt accggttctt ccggcgtacg
    35461 caaatagcga taggtctgaa aacgcggatc cagagttgga ccaacattca gggtgtttaa
    35521 catggcccga ttctgatcat agtcgagatc aaaggccgga ccgacggcct gtaaattttc
    35581 cagtcgagcc tggagcattg gacgcccaag caggaacatt tctgaatcag gtaattcctc
    35641 ggcaggcaca tctgtcgcac tgcgcgaaat attatgctgc tcagcaattt tcagcgcctg
    35701 ctcaatgctg ttcatccggc ggtcgtagat ggctttcgcc acctcttcct gacgcttcac
    35761 ctgagctttc atctggatgg tgcgtgccgc ccatgcgcct ttcagctcct cattcagatg
    35821 gctggctgca cgctggctgg caaaagcaac atactgacgt aacaggttat tagcgtcagg
    35881 cgcggtttcg gcaataagct tcacgctgtc attgaccgcg cgggtaaagt ctccggggat
    35941 aaactggatg ttgttaatca tttcatccag caacgccgca tcggctttgc tgttgcccac
    36001 catccgctgc ttgtaatagt cggtttgcag ccagaactct ctgcgggtat cccacgaggc
    36061 cagttgcata acaaactctt tataggcttc gtccatgacc gatggttggt cggcagaagc
    36121 catgtttgaa cggacatcca ggttacgcaa aaattgctgc tgcgagtaat atccccccag
    36181 catattcacc gttggacgat cggtaatcgc cgtcgcgctc cactcttgtc gagcaaaaaa
    36241 agtatacgcc agcgcgatta acgcaaacgc cagccccatg ccaataatcc atagcttccc
    36301 agcccacaag gtacgaaaca acccacgaat atccagttca ttttcagcgt cttcggccgg
    36361 tttcccaggc attggttgtg tcatcacatc ctcatttatt tggttaaatt ggggctgcca
    36421 ccacgatttc tacgcagtct gcgttttacg cgcttaataa agcgagcaac tttccaggca
    36481 cgcttaatgc aatatccata gaggaagaat gctagcaaaa agagcaccag catgacccac
    36541 tccgggacaa aatgagaata ttctgccagc acgccaatgg aagcgagcag tgctgcggca
    36601 agggtaatca gcacaaacgc ctgacgggaa gtaaacccgg cacgcatgat caaatggtga
    36661 atatgctgac ggtcaggaga gaatgggctc atgcctttac gcaggcgacg gtacataatc
    36721 gccaccatat ccattagcgg aatggcgatt atccacaaag cggtaaccgg gctgatggga
    36781 tgggttttgc cctgggtcgt ttcgagcagg atccagataa cggtaaaacc aatcagcgta
    36841 ctgcccgcat cacccataaa gactttgtag cggcgaccca ggataccaag gttaagcatg
    36901 atgtatggca ggatggcggc gatcatcgca aagcaccaga ttgcgaggct ggtttgcccg
    36961 tcgaaccaca aaatcatacc gattgctgca aacgagacgc aggacaaccc gcccagcaag
    37021 ccatcaatgc catcaaccat gttgaacgca ttaatggccg cccagacggc aaatagcgtc
    37081 aggaagtaac caaacggtcc gagcaccatc tcccaggagc caaagatata acccaggcta
    37141 ctgagataaa gtttgccgaa caccatcata acaatgccaa cagcggcctg tatggtggca
    37201 cggattttta cgctgatatc aaaacggtca tccagcgcgc caatgaaaac aagcacaccg
    37261 gcacaagcga gatagagaga tgcatgcgga atatagtaat cgacaattcc gaacgtgaag
    37321 caaatccctg cgtaaaccga aatcccccca acgagaggta tcaatccctg gtgacgtttg
    37381 cggaagtttg gtttatccac taaaccgact ttttttgcca ctttacgggc aaaaaacaga
    37441 aacagtgtcg tgaataaaaa aatactgatg agatcagtac tcactgtcag taaattcaca
    37501 atgcatgctc tcagagaaaa ttattagcag aagtataacc acgaagacct ttattcagaa
    37561 gggaaagtcc gttccgaacc caccagtcct gtgcaaaaat taaccatttg atatgcaaat
    37621 tgcataacat gccagcaaat tccaggaatt ttcctaacat cgccagcgcg gcattaagat
    37681 tacagatata aaagcaaaac gccacgtaaa cacgtggcgt ttttggcata agacaaattt
    37741 atgagcgttt catcatttcg aagaaatcgt cattggtctt agtcatggcc agtttattaa
    37801 tgaggaattc cattgcatcg atttcgccca tcgggtgaat gattttgcgc aggatccaca
    37861 ttttctgcag ttcttcctga gtcgtgagca gctcttcttt acgggtaccg gaacggttgt
    37921 agtcgatagc cgggaagacg cgtttttcag cgatcttacg agagaggtgc agttccatgt
    37981 tgcctgtacc tttaaactct tcgtaaataa cttcgtccat tttagaaccg gtatcgataa
    38041 gcgccgtcgc gataatggtc aggctgccgc cctcttccac gttacgcgcc gcaccgaaga
    38101 agcgtttcgg acgatgaagg gcattggcgt ccacaccacc ggtcaacact ttacctgacg
    38161 ccggaacaac agtgttgtaa gcgcgcgcca gacgagtgat ggagtcgagc agaatgataa
    38221 cgtctttctt gtgctcaacc aggcgtttgg ccttctcgat caccatttcc gcaacctgaa
    38281 cgtggcgaga tgcgggttcg tcaaaggtag aagcaacaac ttcacctttt accagacgct
    38341 gcatctcggt tacttcttcc ggacgttcgt cgatcagcag aaccatcagc acacaatccg
    38401 ggtggttgta agcaatgctc tgagcaatgt tctgcagcag catagtttta ccggctttcg
    38461 gcggtgccac aatcagacca cgctgaccac gaccgatagg tgatgccaga tccagtacgc
    38521 gagcggttaa atcttcagta gaaccgttac cacgttccat acgcagacga gagtttgcgt
    38581 gcagcggggt taagttctca aagaggattt tgttgcgggc gttttcaggt ttgtcgaagt
    38641 taacttcgtt aactttcagc agcgcaaaat agcgttcacc ttctttcggc gggcgaatct
    38701 taccagagat ggtatcacca gtgcggaggt tgaaacggcg gatttggcta ggggaaacgt
    38761 agatgtcatc aggaccggcg aggtaggagc tgtctgcgga acggaggaaa ccaaatccat
    38821 cctgcaatat ctccagtacg ccatcaccaa agatatcttc gccactcttt gcgtgctgct
    38881 tcaggatggc aaaaataatg tcctgcttac gcatacgagc caggttttcc agccccatat
    38941 tttcgccgag agtgatcagc tcagaaaccg gcgtattctt taattcggta agattcatag
    39001 tggtgtgagt tcttaaactt ggggtaaatc tcgaacttaa tgttgtgaat ggtatggcag
    39061 gatcatccat gcctgtttac ggtcatcgac tcatgtcttt tcgctgcctg gtcacaggag
    39121 agtacgcaga actgaaacga caagacggat tgagtgacga gccagaaatc tgttcacttc
    39181 gcatttaaga taaaacggga agcgttggaa attacaagat tcaaacttaa taaggtatgt
    39241 ttaatacgaa gtcaacacta agttagcatg actcacgccg ggcgtccagt ttttagcgac
    39301 ggggcacccg aacatgaaat tcccttacgc caggttagcg tcgaggaact ctttcaactg
    39361 acctttagac agtgcaccca ctttggttgc cgccacttca ccgtttttga acagcagcag
    39421 agtcgggata ccacggatgc catatttcgg cgcagtgcca gggttttgat cgatgttcag
    39481 ttttgcaacg gtcagtttgc cctgatattc gtcagcgatt tcatccagaa tcggggcgat
    39541 cattttgcac ggaccgcacc actctgccca gaaatcgacg aggatcgccc cgtccgcttt
    39601 gagtacatcc gtgtcaaaac tgtcgtcagt caggtgaata attttatcgc tcatatataa
    39661 ctccacagga ataagcctgg cgtgttggtt tcgttgttgg tgtaacatta accaactaaa
    39721 ggttgacttt atttcaccgg atacgctttc gtaaagcaat agtaagctga tattctacca
    39781 cactatgagc aaaacacatt taacagaaca gaagttttcc gacttcgccc tgcatccgaa
    39841 ggttgtagaa gcccttgaaa aaaaagggtt tcataactgt acgcccattc aggcactggc
    39901 ccttccgctg acgctggcgg gtcgtgacgt agccgggcag gcgcaaaccg gtaccgggaa
    39961 aacgatggcg tttcttacgt caacgtttca ttatcttctc tctcatcctg cgattgccga
    40021 tcgcaaggtg aatcaaccac gtgccttaat tatggcaccg acgcgtgaac ttgccgtgca
    40081 gatccatgcc gacgcagaac cgctggcgga agctaccggc ctgaagctgg gtctggctta
    40141 cggtggtgat ggctacgaca aacagctgaa agtgctggaa agcggcgttg acattctgat
    40201 tggcactacg gggcgtttaa ttgactacgc caagcagaac cacattaacc tcggtgccat
    40261 tcaggtggtg gtactggacg aagccgatcg catgtacgat ctgggcttta ttaaagatat
    40321 ccgctggctg ttccgccgta tgccgcctgc aaaccagcgc ctcaacatgc tgttctccgc
    40381 cacgctttcg taccgggtac gtgaactggc gttcgagcag atgaacaatg ccgaatatat
    40441 tgaagtggaa ccggaacaga aaacgggcca ccgtattaaa gaagagcttt tctacccttc
    40501 taacgaagaa aaaatgcgtt tgctgcaaac gctgatcgaa gaagagtggc cagacagagc
    40561 gattattttc gccaatacca aataccgttg tgaagagatc tggggccacc tggcggcaga
    40621 tggtcatcgt gtcggtttgt tgacaggcga tgtcgcgcag aaaaaacgtc tgcgtattct
    40681 tgatgaattt acccgtggcg atctggatat tctggttgcc accgacgttg ccgcgcgtgg
    40741 tttgcatatt ccggcagtga cgcacgtctt taactacgat ttacccgatg actgtgaaga
    40801 ttacgttcac cgtattggtc gtacaggtcg cgcaggcgca agcggtcact ctatcagcct
    40861 ggcgtgtgaa gagtatgcat tgaatttgcc tgctattgag acctatattg gtcactcaat
    40921 tccggtaagc aaatacaatc cggacgcatt gatgaccgat ctgccaaaac cgctgcgcct
    40981 cacgcgccca cgtacaggca atggtccgcg tcgtactggc gctccgcgta atcgtcgtcg
    41041 ttcaggttaa ataaaatgtc atactcaaaa tcattcaaac tgtatcaaga tggcaagaga
    41101 atgaatcccg atgagcatac tgtagtaagt gattcaggtg aaagaatgca gccaacacag
    41161 agacagattg aaggatgaag agtatgggtt ccacctcgtc gctgtatgca gccattgatc
    41221 tcggttcgaa tagttttcat atgctggttg tgcgcgaggt ggctggaagc atccagacgc
    41281 tgacgcgaat aaaacgcaaa gtgcgtctgg ctgctggcct gaatagcgaa aatgccctgt
    41341 ctaatgaagc aatggagcgc ggttggcaat gtctgcgcct gtttgctgaa cgtctgcaag
    41401 atatccctcc ctcgcaaatt cgcgttgtcg ctacggcgac attacgtctt gccgtcaatg
    41461 cgggtgattt tattgccaaa gcgcaggaaa tcctcggttg tccggtacag gtgatcagcg
    41521 gtgaagagga agcacgtctc atttatcagg gcgttgctca cacaactggc ggtgccgatc
    41581 agcgcctggt ggtggatata ggcggtgcca gtactgaact ggtaaccggc acgggtgcac
    41641 aaaccacctc gttgttcagc ctgtcgatgg gctgcgtcac ctggctggaa cgctattttg
    41701 ccgatcgtaa tctggggcag gaaaattttg atgctgcaga aaaagcggca cgcgaagtgt
    41761 tacgtccggt tgccgatgaa ttacggtatc acggctggaa agtgtgcgtt ggtgcttccg
    41821 gcaccgtgca ggcgttacag gaaatcatga tggcacaggg gatggatgaa cgcattaccc
    41881 tggaaaagtt gcagcaattg aaacagcgag ccattcattg cggtcggctg gaggaactgg
    41941 agattgacgg gctgacgctg gaacgtgcgt tagtgttccc gagtggtctg gcgatcctga
    42001 tcgccatttt taccgaactg aatattcagt gtatgaccct ggcgggcggt gcgctgcgtg
    42061 aaggcctggt ctacggcatg ttgcatctgg ccgtcgagca ggatattcgc agccgtacgc
    42121 tgcgtaatat tcagcgccgc tttatgatcg atattgatca ggcgcagcgc gtagccaaag
    42181 ttgcggctaa cttcttcgat caggtggaaa aggaatggca tcttgaagca ataagccgcg
    42241 atttgctcat cagcgcctgt cagcttcatg aaatcggcct gagcgttgac tttaaacaag
    42301 cgccgcaaca cgctgcttat ctggtgcgta atctggatct tcccggtttt acccccgcac
    42361 agaaaaaatt gctggcgacg ctactgctca accagactaa tccggtcgat ctctcatcgc
    42421 tgcatcagca aaatgccgta cctccgcgcg tcgcagaaca actctgccgt ttactccgcc
    42481 tggccatcat ttttgccagc cgtcgccgtg acgatctcgt gccagagatg acattacagg
    42541 ctaaccatga actgttgacc ttgacgcttc cgcaaggttg gctaacccaa catccgctgg
    42601 gtaaagagat tattgctcag gaaagccagt ggcagagcta tgtccactgg ccgctggaag
    42661 tgcattaatg agtaagtgcc ggatgcgatg ctgacgcatc ttttccggcc ttgattattt
    42721 ccctcgtttt gccgccatca tcgctttcag attcgccaga tggctttgcc ctttctgcat
    42781 ccgttcttcg gcgctgacca ctttgcgctc ctgttcccaa atcagatcat cctgcggcag
    42841 ctccagcaaa aagcggctcg gctccgggcg caccagctcg ccgtactgac ggcgttcttt
    42901 acacagcgta aaggtcaatt ccttctgggc gcgggtaatg ccgacatagg ccagccgccg
    42961 ctcctcatcg atattatctt catcgatgct gctctggtgc ggcaaaaacc cttcttccat
    43021 accgaccatg tagacataag gaaactccag cccttttgac gcgtgcagag tcatcagttg
    43081 cacctgatcc agctcttctt cactctcacc acgctccatc atgtcgcgca aagtaaagcg
    43141 cgtcaccacc tgggtgagcg tcatcggctc atccagttcg ctgccttcca gcatctccgt
    43201 catccagcta aacagttggt tgacgttctt catgcgcatt tcggcggctt tcgggctggg
    43261 cgatgtttcg tacagccagg attcataatc catgccgtgg atcagatcgc gcaccgcggc
    43321 aatcggctcc cgctccgcca gacgctggat ttctgccaac cagtgagtaa agcgggtcaa
    43381 tgcttcataa ccacgtccgc taagcgtctg actcaggccc atatcaaagc tggcggtaaa
    43441 catgctttta ttgcgcgtca tcgcccactc acccagcttt ttcagcgtcg ccgggccaat
    43501 ctctctcttc ggcgtgttaa cgatacgcag aaatgcgctg tcatcgtccg ggttagtcag
    43561 cacgcgcaga taagccagca agtccttgat ttcaggacga gagaaaaacg acgtaccacc
    43621 agaaattttg tacgggatgc ggttttgcat caggaatttt tcaaacactc gcgactgatg
    43681 gttaccgcga taaagaatag cgtaatcttt gtactgcgtt ttattgacga agtgatgcgc
    43741 aatcagttca ccagtgacgc gctcggcttc atgttcttcg ttattcgcgc ttaaaacttt
    43801 aagctctgtg ccgtaaccca gttcggagaa cagacgcttc tcaaagacgt gcgggttatt
    43861 ggcgatcagg atgttcgccg ctttcaaaat acgtccggaa gagcggtagt tctgctcaag
    43921 cttaatcacc ttcagcgccg gaaaatcctg actcagcagc accaggtttt gtggacgtgc
    43981 gccgcgccag gagtagatcg actggtcgtc atcgcccacc acggtaaagc gcgcgcggct
    44041 gcccaccagc agtttcacca gttcatactg gctggtgttg gtgtcctgat actcatccac
    44101 cagcagatag cgaattttgt tctgccagcg ctcgcggact tcttcattgc gttgcagcag
    44161 caacgtcggc aataaaatca gatcatcgaa gtcgagaaca ttgcacgctt tcaggtgtgc
    44221 atcatacagc ccataacaat gggcaaaaat acgatcccgc tcgccctttg cctctgccgc
    44281 cgcctgcgcc ggagttttga gatcgttctt ccagttggag atggtcgaaa tcagctgttg
    44341 caggagaact ttgtcatctt caatcagccc ctcggtcaac tctttaagca aagcaagctg
    44401 atcggtatcg tcaaacagcg aaaagttcgc cttcatccca agcgccgcat actcgcgttt
    44461 gatgatatcc aaccccagcg tgtgaaaagt ggagatcatc aacccgcgcg cctctttgcg
    44521 acccagcgtc tgcccgacac gctctttcat ctcgcgcgcc gccttattgg taaaggtcac
    44581 cgccgcgata tgtcgcgcct gataaccgca accgcggatc agatgggcga ttttattggt
    44641 gatcacacga gttttaccgg aacccgcgcc cgccagcacc aggcaggggc cggtaacgaa
    44701 ttcgacagct tgttgttggc cggggtttag acgcataggt gtattgctca atcttcgaac
    44761 ggggggagga ttgtagcaga atcgcatttg aatgtgaccg cgtgtaagcc gctaaccggt
    44821 tggctgcgga tgtttaaatc agagagagaa gaccgaaatc ggagttcatc gtgaatccat
    44881 tagacttaga aaatatcggg tcgtaacgtt gtgttattac tttttcagag tttactaagg
    44941 agaggtttac tcgtggcgat taatttcagc cctaaggttg gtgaaataca ggaatgcaac
    45001 tttggtaact atcctgttat ccagaatgga cagttcagca ccacgtatta cgatggccgc
    45061 attcctcctg aaatgataaa aaacaggtta gttgtcgtgc tcaacggaaa aattaatggc
    45121 aacgcatgta tcgtcgttcc attatcaacc actcgcgatc atgacaagtt aaatcgtgga
    45181 atgcatgttg aaatagccag taatgttatc aatggcctgc aattctttga tcagcagatt
    45241 cgttgggcca aagccgacct ggtacaacaa gtcagtcgaa accgattaaa tagagcaagg
    45301 acttacagag ggtatcttaa tcaatgtttg ccacatgagt tggttgcaga catacagagg
    45361 gcggtcatca agtcaatcaa tgccatatcg ctcataaatt gacaataaaa taaccatccc
    45421 cgataatgac tttgtagccc atagaggccc taagttttct ccccttgagg gacctgcgaa
    45481 gcccggattt tccgggcttt tgccatatat gtaccagccc ctccctgact gacaattacg
    45541 ttagcatctg cggatcatgc taaaatcgcc gcctgacaat tattcactca aggcacgatc
    45601 atggcaaaaa cagcagcagc actgcatatc cttgtaaaag aagagaaact ggctctggat
    45661 cttctcgagc agattaagaa cggtgccgat ttcggcaagc tggcgaagaa acactctatt
    45721 tgcccgtcag gcaaacgcgg cggtgattta ggtgaattcc gccagggtca gatggttccg
    45781 gcgttcgata aagtggtttt ctcttgtccg gtactggagc cgaccggccc gctgcacacc
    45841 cagttcggat atcacatcat taaggtgctg taccgcaact aatagcaagg ccttctccag
    45901 gagaaggcct tgagtgtttt ctccctctcc ctgtgggaga gggtcggggt gagggcatca
    45961 gcgcgcactt aacccgcaac agcaatacgt ttcatatctg tcatatagcc gcgcagtttc
    46021 ttacctacct gctcaatcgc atggctgcga atcgcttcgt tcacatcacg cagttgcgcg
    46081 ttatctaccg cgccttccgg aatagcttta cccaagtcgc ccggttgcag ctctgccata
    46141 aacggtttca gcaacggcac acaagcgtaa gagaacagat agttaccgta ctcagcggta
    46201 tcagagataa ccacgttcat ttcgtacaga cgcttacggg cgatggtgtt ggcaatcagc
    46261 ggcagctcgt gcagtgattc ataatatgca gactcttcaa tgatgccgga atcgaccatg
    46321 gtttcgaacg ccagttcaac gcccgctttt accatcgcga tcatcagtac gcctttatcg
    46381 aagtactcct gctcgccgat tttgccttca tactgcggcg cggtttcaaa cgcggttttg
    46441 ccggtctctt cacgccaggt cagcagtttc ttatcatcgt tggcccagtc cgccatcata
    46501 ccggaagaga attcgccgga gatgatgtcg tccatatgtt tctggaacag cggtgccatg
    46561 atctctttca gctgttctga cagcgcataa gcacgcagtt tcgccgggtt agagagacgg
    46621 tccatcatca gggtgatgcc gccctgcttc agcgcttcgg tgatggtttc ccaaccgaac
    46681 tgaattagtt tttctgcgta tgccggatcg gtgccctctt ccaccagctt gtcgaagcac
    46741 agcagcgagc cagcctgcaa cataccgcac aggatggttt gctcacccat caggtcagat
    46801 ttcacttccg caacgaagga cgattccagc acgcccgcac ggtgaccacc ggttgctgcc
    46861 gcccatgctt tagcgatcgc cataccttcg cctttcggat cgttttccgg gtgaacggca
    46921 atcagcgtcg gtacgccgaa tccacgtttg tactcttcac gcacttcggt gccagggcat
    46981 ttcggcgcaa ccatgactac ggtgatatct ttacggatct gctcgcccac ttcgacgatg
    47041 ttgaaaccgt gcgagtaacc cagcgccgcg ccgtctttca tcagtggctg tacggtgcgc
    47101 actacatcag agtgctgctt gtccggcgtc aggttaacca ccagatccgc ctgcgggatc
    47161 agttcttcgt aagtaccaac tttaaaaccg ttttcggtcg ctttacgcca ggacgcgcgc
    47221 ttctcggcaa tcgcttcttt acgcagagcg taggagatat ccagaccaga atcacgcatg
    47281 ttcaggccct ggttcagacc ctgtgcgcca cagccgacga tgactacttt tttaccctga
    47341 aggtagctcg cgccatcggc gaattcatcg cgccccataa agcgacattt gcccagctgt
    47401 gccagctgct gacgcagatt cagtgtattg aagtagttag ccatggtgat tcctcgtgat
    47461 gttgtgcttc ttattgttcg gttcgcgttt gcgagattga attcactata tgacaggaaa
    47521 tttattgcgg aaattgatat attcacaacg tcacattgca atttttgcaa cgtcaacatc
    47581 gagggctgcg ccattggatt tacgcgatct gaaaaccttc ctgcatctgg cggaaagccg
    47641 ccattttggc cgcagcgcgc gggcgatgca cgttagccca tccacgctct cacggcagat
    47701 tcagcgcctg gaagaagatc ttggtcagcc gctatttgtg cgcgataacc gcacggtaac
    47761 gctgacggaa gcgggcgaag agctgcgcgt tttcgcccag caaacgctgt tgcagtatca
    47821 gcaattgcgc cacaccatcg atcagcaagg gccgtcgctc tctggcgaat tacatatctt
    47881 ctgctcggtg accgctgcct acagccatct gccgccgatt ctggatcgct tccgcgcgga
    47941 acacccgtcg gtggagatca aactcaccac tggtgatgcg gcagatgcaa tggaaaaagt
    48001 ggtcacggga gaagcggatc tggcgattgc cgggaaaccg gagacgctac ccggcgctgt
    48061 ggcgttttcg atgctggaga atctggcagt agtgctgatt gcgcccgcgc tgccctgccc
    48121 ggtgcgtaac caggtttcgg cagagaagcc ggactggtct acagtgccgt ttatcatggc
    48181 cgatcaggga ccggtgcgtc gccgcattga actgtggttt cgacgcaata aaatcagcaa
    48241 cccgatgatt tacgccacgg ttggcgggca tgaagcgatg gtatcgatgg tggcgctcgg
    48301 ctgtggtgtg gcgctgttgc cggaagtagt gctggaaaac agccccgaac cggtgcgtaa
    48361 ccgcgtgatg attttagagc gcagcgatga aaaaacgccg tttgaactgg gcgtgtgtgc
    48421 acaaaaaaag cggctgcatg agccgctgat tgaggcgttc tggaggattt tgccgaacca
    48481 caaatgacgt tgtcgcgcgg gtaggcctga taagcgaagc gctatcaggc atttttccct
    48541 aacccgccaa aaagaacctg aacgccgggt tattggtttc gtcgtggcaa tcgtagccca
    48601 gctcattcag ccgggtttcg aaatccggtt catggtcgcc aagttcgaac gccgccagta
    48661 cgcgcccgta gtcggtgcca tggctgcgat agtggaacaa agaaatgttc cagtgcgtac
    48721 ccagcgtgtt gaggaagcgc agcagcgcgc ccggtgattc cgggaattcg aagctgtaga
    48781 ggcgttcctg caacggatgt gacggacgcc cgccgaccat atagcgcaca tgcaacttcg
    48841 ccatttcgtc gtcggagaga tccaccacgc tgtagccgcc gtcgttgagc atctgcaaaa
    48901 tttctttgcg ctcttcgagg ccgcggctca ggcgcacgcc gacaaagatg caggcgtttt
    48961 tggcatcggc aaaacggtag ttgaactcgg tgaccgatcg cccgccaagc agttggcaga
    49021 atttgaggaa gctgcctttt tcttccggaa tggtcaccgc caacaacgct tcacgctgtt
    49081 cgcccagttc gcagcgttcc gagacgtagc gcaggccgtg gaagttcacg ttggcaccgg
    49141 aaagaatatg cgccagccgt tcgccgcgaa tgttgtgctg ggcgatatat tttttcattc
    49201 ccgccagcgc cagcgcgcca gagggttccg ccaccgcgcg cacatcttcg aataaatcct
    49261 tcatcgccgc acagatcgca tcactatcga cggtgatgat gtcgtcgaga tactcctggc
    49321 ataaacggaa ggtttcgtca ccgatgcgtt ttaccgctac gccttcagca aatagcccta
    49381 cgcgcggcag atcaaccgga tgacccgcat ccagcgctgc tttcaggcag gcggagtctt
    49441 ccgcttctac ggcgatcact ttgatttgtg gcatcagttg tttgatcagc accgcaacgc
    49501 ccgccgccag accgccgccg ccgactggca caaatacgcg gtcgagatgg gcgtcctgct
    49561 ggagcagttc cagcgccagc gtgccttgcc cggcaatcac catcggatgg tcgaacggcg
    49621 gcacccaggt gaacccctgc tgctgtgaca gttcgatcgc tttagctttc gcttcgtcaa
    49681 agttcgcgcc atgaagcagc acttcgccgc cgaatccacg taccgcatcg actttgatgt
    49741 cggcggtggc ggttggcata acgatcaggg ccttcacgcc taaccgcgca gaagaaaacg
    49801 cgacgccctg cgcgtggtta cccgcagaag cagtgatcac gccgtgcgct ttttgttctt
    49861 ccgtcaggcc cgccatcatg gcgtatgcgc cgcgcagctt aaagctgtgc actggctggc
    49921 gatcttcgcg cttcaccaga atgacgttat caagacgcga cgacagtttt tccatttttt
    49981 gtagcggcgt aacctgcgcc gcctcgtaaa ccggcgcgcg cagcactgct cttaaatatt
    50041 cggcaccttc cggagcaccg gacaggggtt gcgagtcagc cattattaac cccccagttt
    50101 cgatttatcg cgcaccgcac ctttgtcggc gctggttgcc aggctggcgt aggcgcgcag
    50161 ggcaaaggaa acctgacgtt cacggttttt cggcgtccag gctttgtcac ctcgagcgtc
    50221 ctgcgcttca cgacgcgccg ccagttcggc atcgcttacc tgtaactgaa tgccacggtt
    50281 cgggatgtct atagcgatca ggtcaccatc ttcaatcagg ccaatgctgc cgccgcttgc
    50341 cgcttccggt gagacgtggc cgatggaaag accagaggtg ccgccagaga aacgaccgtc
    50401 ggtgatcagc gcacaggctt taccgagacc cattgatttc aggaagctgg ttgggtagag
    50461 catttcctgc atgccagggc cgcctttcgg gccttcatag cggatcacca ccacatcgcc
    50521 cgcgacaact ttgccaccga ggatcgcttc taccgcgtcg tcctggcttt cgtacacttt
    50581 cgccgggccg gtgaatttga ggatgctgtc atcgacgccc gcggttttaa cgatgcagcc
    50641 gttttctgcg aaattaccgt agagcaccgc caggccgccg tctttgctgt aggcgtgttc
    50701 cagcgagcgg atacagccat tggcgcgatc gtcatcgaga gaatcccaac ggcaatcctg
    50761 cgagaatgcc tgtgtggtac gaatgcctgc aggacctgcg cggaacatat tttttaccgc
    50821 gtcatcctgg gtcagcataa cgtcgtattg ttccagcgtt tgcggcaacg tcaggccaag
    50881 tacgtttttc acatcacggt tcagtaaccc cgcgcgatcc agttcgccga gaataccgat
    50941 aacaccacca gcacggtgaa catcttccat atggtatttc tgggtgctcg gcgcaacttt
    51001 acacagctgt ggaaccttgc gggaaagctt atcgatatca ctcatggtga agtcgatttc
    51061 cgcttcctgc gccgccgcca gcaggtgaag tacggtgtta gtcgatccac ccatcgcgat
    51121 atccagcgtc atggcgtttt caaacgccgc tttgctagca atattgcgcg gcagtgcact
    51181 ttcgtcgttt tgctcgtagt aacgtttggt caattcaaca atgcgtttac cggcattgag
    51241 gaacaactgc ttacggtcgg cgtgggttgc cagcagcgag ccgttgcccg gctgcgacag
    51301 acccagcgct tcggtcaggc agttcattga gttagcggta aacatcccgg agcaggagcc
    51361 gcaggtcgga cacgcggaac gttcaacctg atcgctctgg gagtcagaaa ctttcgggtc
    51421 tgcgccctgg atcatcgcat caaccagatc gagtttgatt atctgatcgg acagtttggt
    51481 tttcccggcc tccatcgggc cgccggaaac aaagatcacc ggaatattca ggcgcaggga
    51541 agccatcagc atccccgggg tgattttgtc gcagttagag atgcagacca tggcgtcggc
    51601 gcagtgggcg ttgaccatat actcaacgga atcagcgatc agttcgcgag atggcagtga
    51661 ataaagcatc cccccgtggc ccatggcaat cccatcatcc accgcaatgg tgttgaactc
    51721 tttggcaacg ccgccagccg cttcaatttg ttcggcgacc agtttaccga gatcgcgcag
    51781 atggacgtga cccggtacaa attgggtgaa cgagttcaca accgcgataa tcggcttgcc
    51841 aaaatcggcg tcggtcattc cggtggcgcg ccacagcgca cgagcacccg ccatattacg
    51901 accatgagtg gtggtggcgg aacggtactt aggcatactt tatttactcc cagtgtctgt
    51961 ctcgtaaatg ggacggtgcg tgccgtccca ttttttgtat ttattgatta acttgatcta
    52021 accagcccca tttatcttcg gtttcgccag tgaagaggcc gaagaaggct tgctgaatgc
    52081 gtttggtaac cgggccacaa cggccttcgc caacctgaat accatctaca ctgcgcactg
    52141 gcgtgatttc tgccgcagta ccggacataa acacttcatc cgccaggtac agggattcgc
    52201 gcgacagaac ctgctcacgc acttcaattc ccagctcttt cgccagtttg atgatggcat
    52261 cacgggtaat acccggcagc gcggaggagg tgaacggtgg ggtgaacagc acaccatctt
    52321 tcacttcaaa caggttttcg cctgcgcctt cagagatata accgttcaca tccagcgcga
    52381 taccttcctg ataaccgtgg cggcgcgctt cgctacccac cagcagggaa gagagatagt
    52441 taccaccggc ttttgccgcg gttgggatgg tgtttggtgc tgcgcggttc caggaggaaa
    52501 ccatcgcatc gatcccctgc tccagcgctt ctgcgcccag atacgctccc cacgggaaag
    52561 cggcgataat cacatcggtt gagtatcccg ctggcgggtt tacacccatg ccaacatcac
    52621 caacgaagat cagcggacgg atataggcgc tggtgagatt gtttttgcgg atcacgtcac
    52681 gacaagcttc catcagctca tcaatgctct gcgaaaccgg gaagcgatag attttggcgg
    52741 agtcatgcag acgctgcata tgctcacgat ggcggaatac aaccggtcct ttgtgcgagt
    52801 cgtagcaacg gatgccttca aaaaccgaag tgccatagtg cagcgcgtgc gacatcacat
    52861 gcaccttcgc gtcttcccag cgaaccatct ccccattgaa ccaaatgtaa tcagctttct
    52921 tcgtggtcat ttttatattc cttttgcgct caggcgcgga tttgttgtga tgtggttgtg
    52981 ctctggcaga tggcaacgtg tgcgacgtcc accagtttat ttaactgact aaacagtaag
    53041 tcgaccgacc gtgggctggc aacggtcaat tcgatattta tattttgtgc atcgctggcg
    53101 gcagccatat tcattgagca gacgtggaaa ccacgatgac gcaccacgcg taaaacacgt
    53161 tctaaggttt ccggattgaa gcgagccgat acattgacct gatgttgcat catgataatt
    53221 tctccaacat ttctgaatta ctggcgccag gcggcaccag cggccagacg ttctcaagtt
    53281 cgtcgattga gacatgaagc aggtatggcc catcactgtt cagcatggtg tcgagtgccg
    53341 cttcaacctg gtctttacgg gtgatgtgtt ggccagggat gccgaaggcg ctggctaaca
    53401 tgaggaaatc ggggttatca gtaagggtgg tttcgctgta tcgttcctga aaaaacagtt
    53461 gctgccattg tcgaaccatc cctaaccgtt ggttatcgag taagacgatt ttcaacggta
    53521 actgcttgcg ttttacggtg cccagctctt gcacattcat catgaaagag ccgtcaccgg
    53581 agatacagac gacagtatcg ttcggtcgtg cgacttgtgc gccaaccgcc gctggtaaac
    53641 cgaaacccat ggtgcctaag ccgctggagg taatgaaatt ttccgggcga gtgtgtgcga
    53701 tgtgctgcgc ggcccacatc tggtgctgcc ccacatctgt ggtcacgacg caatccgcag
    53761 gtttacgatc cgacagttgt tttaacaaca acggcgcgta gatagcgtca ccgggatggt
    53821 cgtaacgcca ggtatgttca tcacgcagct gcgcgcagta ttgctgccag tcattgatat
    53881 ttaacggctg ctgtaatgct ggtaacagag catttaaatc accttgtaat gccacatgtg
    53941 cctgacgcag cttgttcatt tctgccgggt cgatatccat atggataaca ctggcgtgtg
    54001 gtgcgaaggt gttcagtttg ccggtcaccc ggtcatcaaa acgtgcaccc acggcgatca
    54061 gcaagtcgca ctcctgcacc gcgaagttcg ccgctttggt gccatgcatt cccagcatgc
    54121 ccaggtagta cggataatct gcttcaactg cgcccagccc tttcagcgtg caggtggcag
    54181 gcatttttgt ggtagcgaga aattctcgta aagcaggaac tgcctgcgcc atacccacgc
    54241 caccaccaac gtacagcatc ggtttttgcg cttttgccag catctggcgc gcttgctcaa
    54301 cttcggcatg tgggaaagtc acttcgtttt caacggtggt gaaccacggt tccaggtcac
    54361 cgctggctag ctggatatct tttgggatat cgaccagaac cggaccagga cgacctgagc
    54421 tggcaacgtc gaatgcttca gccataatgc gcggcaactc ttccagcgac tgcaccagaa
    54481 agctgtgctt ggtacaggct aacgacaatc ccaggacatc cacttcctga aatgcgtccg
    54541 tgccgataaa cggtgcggac acttgaccgg tgatggcaac aacagggata gaatctaaca
    54601 gtgcgtccgc aagcccggtt atcaggttgg ttgcgcccgg accagacgtg gcgatacata
    54661 cgccagtttt gccggtagca cgggcataac cgatagccgc cattgccgca ccctgctcat
    54721 gtcggcacag caagtgctcc acgccgccgt catacaatgc atcgtaaacc ggcataattg
    54781 cgccacccgg ataaccgaaa acggtgttca caccctgtgc ccgcaacgca tgtaccaccc
    54841 actgtgcgcc attcatagtt agttccccgt cctgaatctt gagaaacaga attttgtgct
    54901 gttattcatt gtctgctcct cggttatatt tttaaggtca aaaaaaaccc ccggaccttt
    54961 cggtgcgggg gtcttagttc gttaaggctt gatctctaag cctttcctcg tccaagtgca
    55021 gccccgcacg gtgggataat aatcaccacc acgctaatca tgaccaggct aatcactcgt
    55081 agaagggctg tcattttgtc ttttcttgca tcttgttcga aggaatgcct aaagagttac
    55141 catagatttt gcgaatagca caagatattt tttggccgat ttttatgatg cgtttaataa
    55201 tttcaataga aattgttgtt ttttaaaaag aaaatagtga gtgaaaaaaa ttcaatttca
    55261 gtatgaatgt tgttcccttc ctcgtagttc atcctttgtc cgctgcattt ttgcgagcat
    55321 cattccaccg ccagaacaac tcaacttcac ttgcacaaat aaacaggaat cagccactaa
    55381 aattcacgaa ttagcgggct acagcagcga tatcttatgc acataatagt gactcaagga
    55441 gggcttatgt cactgtcaat tgttcatacc cgcgcagccc tgggagtaaa tgcgccccca
    55501 atcactgttg aggtacatat cagtaaaggt ctacccggct taacgatggt gggcttacca
    55561 gaaacaactg taaaagaagc tcgcgatcgc gtgcgcagcg ccattatcaa tagcggatat
    55621 gaatatccgg cgaaaaaaat caccatcaac ctggctccag ccgatctgcc aaaagaaggg
    55681 ggacgatatg atttacctat cgccattgcg ttgctggcgg cctcagaaca gcttacagcc
    55741 aataagttag atgaatatga attagtcgga gaactggcgc ttacaggcgc tctgcgtggc
    55801 gttcccggcg caatctccag tgcaactgaa gctattaagt cgggcagaaa aattatcgtc
    55861 gcgaaagata acgaagatga agtggggcta attaacggtg aaggatgcct gatagccgat
    55921 catctgcagg ctgtctgtgc gtttctggaa ggtaagcacg ctctcgaacg cccgaaacca
    55981 actgatgcag tatcccgggc gctacaacat gatctcagtg atgttgtcgg tcaggagcaa
    56041 ggaaagcgag gactggaaat taccgccgct ggcgggcaca accttttact gattgggccg
    56101 ccgggaacag gtaaaacaat gctcgccagc cgtattaatg gcctgttgcc agatttaagc
    56161 aatgaagagg cactggagag cgctgcgata ttaagtctgg taaatgctga atcagtacaa
    56221 aaacaatggc ggcagcgccc gttccgctca ccgcatcaca gtgcgtcgtt aactgcgatg
    56281 gtgggcggtg gtgcaattcc agggccaggt gaaatttcgt tggcgcataa cggcgtgctt
    56341 tttcttgatg agctacctga atttgaacgg cgtacactgg atgccttgcg agagccgatt
    56401 gaatccgggc agatccatct ttcacgcaca cgagccaaaa taacctatcc agcccgtttc
    56461 cagcttgttg cggcgatgaa tcccagccct accggacatt atcagggaaa ccataaccgc
    56521 tgcacgccag aacagacatt acgttatctc aaccggctct cggggccctt tctcgaccgc
    56581 ttcgatctct cactggagat cccattacca ccccccggca ttttgagtaa aacggtagtg
    56641 ccgggagaaa gcagcgccac cgttaaacaa cgcgtaatgg ccgccagaga gcgccaattt
    56701 aagcggcaga ataaactgaa tgcctggctg gatagtccgg aaatacgcca attctgcaaa
    56761 cttgagagcg aagatgcgat gtggttggaa ggaacactga tccatctggg gttatcgatt
    56821 cgtgcctggc agcggttatt gaaagttgca cgaaccattg ctgatattga tcagtctgac
    56881 attatcacac gtcagcattt gcaggaggca gttagctatc gtgcgattga ccgtttgctc
    56941 atccatctgc aaaaactact gacataaaaa aagggcattt cgcccttttt attaatcatc
    57001 agaatcggtg tagtcttcag caccttcaac ctgcggttta ccgccggaaa gggtgtgaaa
    57061 acgttttgga cgcttaatac gcgtcatata cttggaccac acgcgttctg cttctgtcac
    57121 tggctcacgt tcgccacggc atactgctac gaagagtttc tcttcctcgg taaccggctc
    57181 gcgtttgcca agatccaact cattgaaggc ataaccatga cgctcaagca gttgtgcctc
    57241 tttgatggtg aaatcaccat gacgagagaa tccacgtgga taatgtttat tgtcgaaata
    57301 tcgattagtc gtcgtaaagc tttccgccat cctgcacgct cctaattctt tgaccgagct
    57361 agttatggcg cggagtatta gttacgcttg acagagtgta aaacaaaaca tttaaatcat
    57421 aacgacaaat aattttgcgg agagcactgt ggatacggaa ttgttaaaaa ctttcctgga
    57481 agttagccga acgcgtcact ttggtcgagc ggctgaatcg ctctatctga cccagtcagc
    57541 agtgagcttt cgaatcagac aactggaaaa tcaactgggt gtgaaccttt tcacccgcca
    57601 cagaaacaat atccgtttaa ccgctgccgg tgaaaaacta ctgccttatg cagaaacgct
    57661 catgagcacc tggcaggccg cccgcaagga ggtggcgcat acctcacgac ataacgagtt
    57721 ttctatcggt gccagcgcct cattgtggga atgtatgctt aatcagtggc tgggacgttt
    57781 gtatcaaaat caggatgccc atacaggctt acagttcgaa gcgcgaattg cccaacggca
    57841 gtctctggta aaacagctgc atgaacgcca gcttgatctt cttattacca ctgaagcgcc
    57901 caaaatggac gaatttagta gtcagttgct gggatatttc actttagcgc tttataccag
    57961 tgccccttca aaactaaagg gagatcttaa ttatctgcga cttgagtggg ggccagattt
    58021 tcaacagcat gaggcaggtt tgatcggtgc tgacgaagtg cccattctga caaccagttc
    58081 tgctgaactg gcacagcaac agattgcgat gcttaatggt tgcacctggc tacccgtcag
    58141 ctgggcgcgt aaaaaaggcg gcctgcatac cgttgtcgat agcacaacac tttcacggcc
    58201 gctttatgcc atatggctgc aaaatagcga taaaaatacg ttgattcgcg atcttttgaa
    58261 aattaacgtg ctggatgaag tgtattaata tgaatggctg gcaaggatgc cggtagaagg
    58321 atttacttcg gagagggtta tttcagataa aaaaaatcct tagctttcgc taaggatgat
    58381 ttctggcagg ggcggagaga ctcgaactcc caacacccgg ttttggagac cggtgctcta
    58441 ccaattgaac tacgccccta attagggtgg cggaacggac gggactcgaa cccgcgaccc
    58501 cctgcgtgac aggcaggtat tctaaccgac tgaactaccg ctccaccgaa ttcttttaca
    58561 accaccggtt ttatgaccgg cttac
//
LOCUS       DAZGJW010000013        58399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZGJW010000013 DAZGJW010000000
VERSION     DAZGJW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.013"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..383)
                     /gene="rfbC"
                     /locus_tag="ACHKIK_000847"
     CDS             complement(<1..383)
                     /gene="rfbC"
                     /locus_tag="ACHKIK_000847"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFO9082066.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSEIA"
     gene            complement(388..1266)
                     /gene="rfbA"
                     /locus_tag="ACHKIK_000848"
     CDS             complement(388..1266)
                     /gene="rfbA"
                     /locus_tag="ACHKIK_000848"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO9082067.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGLRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNYVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(1324..2223)
                     /gene="rfbD"
                     /locus_tag="ACHKIK_000849"
     CDS             complement(1324..2223)
                     /gene="rfbD"
                     /locus_tag="ACHKIK_000849"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFO9082068.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWLIHY
                     STDYVFPGNGDMPWRETDATAPLNVYGETKLAGEKALQEYCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQKFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(2223..3308)
                     /gene="rfbB"
                     /locus_tag="ACHKIK_000850"
     CDS             complement(2223..3308)
                     /gene="rfbB"
                     /locus_tag="ACHKIK_000850"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO9082069.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEQSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVENVKSGAYQSWIAQNYEGRQ"
     gene            complement(3680..4573)
                     /gene="galF"
                     /locus_tag="ACHKIK_000851"
     CDS             complement(3680..4573)
                     /gene="galF"
                     /locus_tag="ACHKIK_000851"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO9082070.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGELSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSK"
     gene            complement(4748..6142)
                     /gene="wcaM"
                     /locus_tag="ACHKIK_000852"
     CDS             complement(4748..6142)
                     /gene="wcaM"
                     /locus_tag="ACHKIK_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO9082071.1"
                     /translation="MPFKKLSRRTFLTASSVLAFLHTPFARALPARQSVNINDYNPRD
                     WIASFKQAFSEGQTVVVPAGLVCLNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ACNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNVEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(6153..7373)
                     /gene="wcaL"
                     /locus_tag="ACHKIK_000853"
     CDS             complement(6153..7373)
                     /gene="wcaL"
                     /locus_tag="ACHKIK_000853"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO9082072.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQILRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(7370..8650)
                     /gene="wcaK"
                     /locus_tag="ACHKIK_000854"
     CDS             complement(7370..8650)
                     /gene="wcaK"
                     /locus_tag="ACHKIK_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO9082073.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDSGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKSLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLELMKRSNITTSKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTIGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQVMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVKSVLEHIREAK"
     gene            complement(8796..10274)
                     /gene="wzxC"
                     /locus_tag="ACHKIK_000855"
     CDS             complement(8796..10274)
                     /gene="wzxC"
                     /locus_tag="ACHKIK_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO9082074.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVACVAVFLLSDAI
                     GDMLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPALHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWSSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(10276..11670)
                     /gene="wcaJ"
                     /locus_tag="ACHKIK_000856"
     CDS             complement(10276..11670)
                     /gene="wcaJ"
                     /locus_tag="ACHKIK_000856"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO9082075.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(11806..13176)
                     /gene="cpsG"
                     /locus_tag="ACHKIK_000857"
                     /gene_synonym="manB"
     CDS             complement(11806..13176)
                     /gene="cpsG"
                     /locus_tag="ACHKIK_000857"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO9082076.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(13369..14805)
                     /gene="cpsB"
                     /locus_tag="ACHKIK_000858"
     CDS             complement(13369..14805)
                     /gene="cpsB"
                     /locus_tag="ACHKIK_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO9082077.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPTGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(14808..16031)
                     /gene="wcaI"
                     /locus_tag="ACHKIK_000859"
     CDS             complement(14808..16031)
                     /gene="wcaI"
                     /locus_tag="ACHKIK_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO9082078.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPERIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLHNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGELCETFSGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(16028..16507)
                     /gene="gmm"
                     /locus_tag="ACHKIK_000860"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(16028..16507)
                     /gene="gmm"
                     /locus_tag="ACHKIK_000860"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO9082079.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(16510..17475)
                     /gene="fcl"
                     /locus_tag="ACHKIK_000861"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(16510..17475)
                     /gene="fcl"
                     /locus_tag="ACHKIK_000861"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO9082080.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVMRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(17478..18599)
                     /gene="gmd"
                     /locus_tag="ACHKIK_000862"
     CDS             complement(17478..18599)
                     /gene="gmd"
                     /locus_tag="ACHKIK_000862"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO9082081.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(18625..19173)
                     /gene="wcaF"
                     /locus_tag="ACHKIK_000863"
     CDS             complement(18625..19173)
                     /gene="wcaF"
                     /locus_tag="ACHKIK_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO9082082.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(19189..19935)
                     /gene="wcaE"
                     /locus_tag="ACHKIK_000864"
     CDS             complement(19189..19935)
                     /gene="wcaE"
                     /locus_tag="ACHKIK_000864"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO9082083.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(19946..21163)
                     /gene="wcaD"
                     /locus_tag="ACHKIK_000865"
     CDS             complement(19946..21163)
                     /gene="wcaD"
                     /locus_tag="ACHKIK_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO9082084.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(21138..22355)
                     /gene="wcaC"
                     /locus_tag="ACHKIK_000866"
     CDS             complement(21138..22355)
                     /gene="wcaC"
                     /locus_tag="ACHKIK_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO9082085.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVVKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITCTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(22352..22840)
                     /gene="wcaB"
                     /locus_tag="ACHKIK_000867"
     CDS             complement(22352..22840)
                     /gene="wcaB"
                     /locus_tag="ACHKIK_000867"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO9082086.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(22843..23682)
                     /gene="wcaA"
                     /locus_tag="ACHKIK_000868"
     CDS             complement(22843..23682)
                     /gene="wcaA"
                     /locus_tag="ACHKIK_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO9082087.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(23775..25937)
                     /gene="wzc"
                     /locus_tag="ACHKIK_000869"
     CDS             complement(23775..25937)
                     /gene="wzc"
                     /locus_tag="ACHKIK_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO9082088.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(25940..26383)
                     /gene="wzb"
                     /locus_tag="ACHKIK_000870"
     CDS             complement(25940..26383)
                     /gene="wzb"
                     /locus_tag="ACHKIK_000870"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO9082089.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(26389..27528)
                     /gene="wza"
                     /locus_tag="ACHKIK_000871"
     CDS             complement(26389..27528)
                     /gene="wza"
                     /locus_tag="ACHKIK_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO9082090.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            28187..29770
                     /gene="yegH"
                     /locus_tag="ACHKIK_000872"
     CDS             28187..29770
                     /gene="yegH"
                     /locus_tag="ACHKIK_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO9082091.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFCSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHGIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(30044..31897)
                     /gene="asmA"
                     /locus_tag="ACHKIK_000873"
     CDS             complement(30044..31897)
                     /gene="asmA"
                     /locus_tag="ACHKIK_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO9082092.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(31919..32500)
                     /gene="dcd"
                     /locus_tag="ACHKIK_000874"
     CDS             complement(31919..32500)
                     /gene="dcd"
                     /locus_tag="ACHKIK_000874"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO9082093.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(32592..33233)
                     /gene="udk"
                     /locus_tag="ACHKIK_000875"
     CDS             complement(32592..33233)
                     /gene="udk"
                     /locus_tag="ACHKIK_000875"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO9082094.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            33551..36861
                     /gene="dgcE"
                     /locus_tag="ACHKIK_000876"
                     /pseudo
     CDS             33551..36861
                     /gene="dgcE"
                     /locus_tag="ACHKIK_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(36900..37757)
                     /gene="alkA"
                     /locus_tag="ACHKIK_000877"
     CDS             complement(36900..37757)
                     /gene="alkA"
                     /locus_tag="ACHKIK_000877"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO9082095.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            37891..39243
                     /gene="yegD"
                     /locus_tag="ACHKIK_000878"
     CDS             37891..39243
                     /gene="yegD"
                     /locus_tag="ACHKIK_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9082096.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRERLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(39276..41195)
                     /gene="yegI"
                     /locus_tag="ACHKIK_000879"
     CDS             complement(39276..41195)
                     /gene="yegI"
                     /locus_tag="ACHKIK_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO9082097.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQA"
     gene            complement(41192..41953)
                     /gene="pphC"
                     /locus_tag="ACHKIK_000880"
     CDS             complement(41192..41953)
                     /gene="pphC"
                     /locus_tag="ACHKIK_000880"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFO9082098.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(41950..42609)
                     /gene="yegL"
                     /locus_tag="ACHKIK_000881"
     CDS             complement(41950..42609)
                     /gene="yegL"
                     /locus_tag="ACHKIK_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO9082099.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(42830..42889)
                     /gene="ibsA"
                     /locus_tag="ACHKIK_000882"
     CDS             complement(42830..42889)
                     /gene="ibsA"
                     /locus_tag="ACHKIK_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO9082100.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(43162..43218)
                     /locus_tag="ACHKIK_000883"
     CDS             complement(43162..43218)
                     /locus_tag="ACHKIK_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO9082101.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(43490..43546)
                     /locus_tag="ACHKIK_000884"
     CDS             complement(43490..43546)
                     /locus_tag="ACHKIK_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO9082102.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            43825..45072
                     /gene="mdtA"
                     /locus_tag="ACHKIK_000885"
     CDS             43825..45072
                     /gene="mdtA"
                     /locus_tag="ACHKIK_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO9082103.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            45072..48194
                     /gene="mdtB"
                     /locus_tag="ACHKIK_000886"
     CDS             45072..48194
                     /gene="mdtB"
                     /locus_tag="ACHKIK_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO9082104.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVSTTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIASYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            48195..51272
                     /gene="mdtC"
                     /locus_tag="ACHKIK_000887"
     CDS             48195..51272
                     /gene="mdtC"
                     /locus_tag="ACHKIK_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO9082105.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIVISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            51273..52688
                     /gene="mdtD"
                     /locus_tag="ACHKIK_000888"
     CDS             51273..52688
                     /gene="mdtD"
                     /locus_tag="ACHKIK_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9082106.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            52685..54088
                     /gene="baeS"
                     /locus_tag="ACHKIK_000889"
     CDS             52685..54088
                     /gene="baeS"
                     /locus_tag="ACHKIK_000889"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO9082107.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            54085..54807
                     /gene="baeR"
                     /locus_tag="ACHKIK_000890"
     CDS             54085..54807
                     /gene="baeR"
                     /locus_tag="ACHKIK_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO9082108.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            54998..55330
                     /gene="yegP"
                     /locus_tag="ACHKIK_000891"
     CDS             54998..55330
                     /gene="yegP"
                     /locus_tag="ACHKIK_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO9082109.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            55539..55835
                     /locus_tag="ACHKIK_000892"
     CDS             55539..55835
                     /locus_tag="ACHKIK_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO9082110.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            55837..56133
                     /locus_tag="ACHKIK_000893"
     CDS             55837..56133
                     /locus_tag="ACHKIK_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9082111.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            56236..57597
                     /gene="yegQ"
                     /locus_tag="ACHKIK_000894"
     CDS             56236..57597
                     /gene="yegQ"
                     /locus_tag="ACHKIK_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO9082112.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(57927..58244)
                     /gene="yegR"
                     /locus_tag="ACHKIK_000895"
     CDS             complement(57927..58244)
                     /gene="yegR"
                     /locus_tag="ACHKIK_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO9082113.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT        13743 a        15594 c        14796 g        14266 t
ORIGIN      
        1 gctatctcgc ttaacaccaa aaaaccatgc gcaaacccct cagggatcca caactgacgc
       61 ttattctcag cagacaaatt caccccaacc catttcccaa atgtaggtga cgatttacga
      121 atatcaactg ctacatcaaa tacttcaccg accacacaac gaactaattt accttgagca
      181 tagggttcca gctgatagtg cagtccgcgt aacacaccct tagatgattt agaatgatta
      241 tcctgaacaa attcaacctt ccgccctaca gcttcttcaa aaactttctg attaaagctt
      301 tccataaaga aaccacgctc atcaccaaaa actttcggtt caaaaatcag tacatctgga
      361 atttctgttt taattacgtt cattttatta ataaccttta atcattttca gcagatactg
      421 accataagca tttttcttca acggttcagc taatactttc acctgctcag catcgataaa
      481 ccctttacgg taagcaattt cttccgggca ggaaactttc agcccctgac gttcttctat
      541 tgttgcgata aaattgcttg cctcaataag actttgatgt gtccccgtgt ccagccatgc
      601 ataaccacgt cccatcatgg caacggataa acgcccctgt tccatataaa tacggttaat
      661 atcggtaatt tccagttcac cgcgggcaga aggcttaaga tttttcgcca tttcgacaac
      721 gtagttatca taaaaataaa gcccggttac cgcataatta ctttttggtt gtagcggttt
      781 ttcttccagg ctgatcgccg taccgttttt atcaaactca acgacaccgt agcgttcagg
      841 atcattaacg tgataggcaa ataccgttgc accactttct ttgttaacag cgacatccat
      901 taacttcggc agatcgtgac cgtaaaagat attatcacct agaaccaaag cacaatcatc
      961 accaccgata aactcttcac cgatgataaa tgcctgcgca agaccatctg gactcggttg
     1021 cactttgtac tgaagattta gcccccactg gctcccgtca cccagcagtt gttgaaaacg
     1081 aggagtatcc tgtggcgtac taataatcag aatatcgcga agacccgcta acatcagtgt
     1141 agacaacggg taatagatca tcggtttatc ataaatcggt aacaactgtt tactgacagc
     1201 catagttaca ggataaagac gagtacccga accaccagct aaaataatac ctttacgcgt
     1261 tttcatttca tcattccttt taattcatct tgctccacca tcacgaacaa gatgcaaaaa
     1321 ctattaaatt gctgtagtcg taaataattc gttgagcatt cgtttcacgc caacctgcca
     1381 atcaggcaaa acaagcgcaa atttctgctg aaatttttct gtattaaggc gagagttatg
     1441 cggacgacga gctggtgtag gataggctgt tgttggtact gcgttgagct tgttgagtgc
     1501 aaggggaata cctgctttgc gtgcctcttc aaaaaccagc gcagcataat cgtgccaggt
     1561 tgtggtacca ctggctacca gatggtacaa gcctgcgact tctggtttat tcagtgcgac
     1621 acgaatggca tgtgctgtac aatcagccag cagttcagca cctgttggcg caccaaactg
     1681 atcattaata accgctaatt cttcacgctc ttttgccaga cgtaacatcg ttttggcgaa
     1741 gttatttcct tttcctgcat agacccagct ggtccggaaa ataagatgct ttgcgcaata
     1801 ttcctgtaac gctttttctc cggctaactt ggtttcaccg taaacattta gtggtgcggt
     1861 tgcatccgtc tcccgccatg gcatatcgcc atttccaggg aagacgtaat cagtcgagta
     1921 atgaataagc caggcgccga cttcattggc tgctttcgca atcgcttcga cacttgttgc
     1981 gttaagtaat tgtgcaaact ccggttctga ttctgctttg tctactgcgg tgtgagcggc
     2041 tgcgttgaca ataatatcag gccgaatgct tcttacggtt tcagctacac cttcaggatt
     2101 actaaaatca ccgcaataat cagtagagtg aacatcaaga gcaatcaaat tacccagagg
     2161 tgccagagca cgctgtagtt cccaacctac ctgccctgtt ttgccgaaaa ggaggatatt
     2221 cattactgac ggccctcata gttctgtgca atccatgact gataggcacc acttttcaca
     2281 ttttcgaccc attttgtatt ggacaggtac cattccactg tcttccgaat cccgctctca
     2341 aacgtttcct gcggtttcca gcccaattcg cggctaatct tctcagcatc aatcgcataa
     2401 cggcgatcgt gtcccggacg atcggcaaca taagtaattt gctcacgata agattgctct
     2461 ttcggtacaa tctcatccaa caaatcacaa atagtcagca caacatcgat gtttttcttt
     2521 tcgttgtgtc cgccaatgtt ataagtttca cccgctttac cttcggtgac aacggtatat
     2581 aacgcacgcg catgatcttc aacatatagc caatcgcgaa tttgatcccc tttgccataa
     2641 ataggtaatg ccttaccttc cagagcatta agaattacca gcggaatcaa tttttccgga
     2701 aagtgataag ggccgtagtt attcgaacag ttagtcacaa tggtcggtaa accataggta
     2761 cgtttccacg cgcggactaa atgatcgcta gatgctttgg atgcggaata aggactgctt
     2821 ggcgcgtaag ctgtcgtctc agtaaataag ggtaattctt ctgtattatt tacttcgtca
     2881 ggatgaggca aatcaccata gacttcgtca gtagaaatat gatgaaaacg gaagctattt
     2941 ttcttgtcgc tatcaagagc agaccagtaa ttgcgagcgg cttccaaaag gacataagta
     3001 ccaacaatat tggtttcaat aaatgccgca gggccggtaa ttgaacggtc aacatggctt
     3061 tcagcagcca ggtgcatcac tgcatccggc tgatgctgag caaaaatccg cgccattgca
     3121 gctgcatcgc aaatatccgc atgttcaaaa acatagcgtt cagaatcaga aacatcagca
     3181 agtgattcca ggtttccggc gtacgttaat ttatcgacat taacaacact atcctgcgta
     3241 ttatttataa tgtgacgaac tacagcagaa ccaataaatc ctgcgccacc agtaacaagt
     3301 atcttcactt tattattcca tattacttca gagcatgctg tgaaataagc ggctcttagg
     3361 ttgattaata ggggtattaa tgcacgctac cgcccctggc tttacagcta ccagtgcact
     3421 gcatgcatgt ctacgatgtg acgagcgtta cccactcgcg ctaaacccga aaaattcaaa
     3481 cgctaattgt cttaccaatc cgctctggaa acaaggaaaa tcctggaaaa ctttgactaa
     3541 aatcctattg ctaactcgtt gttattctga ttgtttatat aaaacaacgg caggaatatt
     3601 cgcaacaaat tactttcatc acgaatcttc actgccgtta taattttctt atcaaccgtt
     3661 acatccggtc agattttcat tatttgctta acagcttctc aatgccttta cggaacttcg
     3721 ccccttcttt caggtttcgt aatccatact tcacgaacgc ctgcatatag cccatttttt
     3781 taccgcagtc gtaactgtcc ccagtcatca gcatggcatc aacggactgt tttttcgcca
     3841 gctcggcaat agcatcagtc agttgaatac gtccccatgc accaggctga gtacgttcca
     3901 gttccggcca aatatcggca gaaagtacat agcgaccaac ggccatgatg tctgagtcca
     3961 gcgtttgtgg ctgatccggt ttttcgataa attcaacaat gcggctgact ttaccttcgc
     4021 gatccagcgg ttctttggtc tggatgacag agtattcaga gagctcaccc ggcatacgtt
     4081 ttgccagcac ctggctacgg cccgtttcat tgaaacgcgc aatcatggca gcaaggttgt
     4141 agcgcagcgg gtcggcactg gcgtcgtcga tcacaacgtc cggcagtacc acgacaaatg
     4201 gattgtcacc aatggcgggt cgtgcacaca aaatggagtg gcctaaacct aaaggttcgc
     4261 cctgacgcac gttcataatg gtcacgcctg gcggacagat ggactgtact tccgccagca
     4321 gttgacgctt cacgcgcagt tcaagaagag attctaattc ataagaggtg tcgaagtggt
     4381 tttcgaccgc gttcttggac gcatgagtta ccaggaggat ttctttgatc cctgcagcca
     4441 caatctcgtc aacaatgtac tgaatcattg gcttgtcgac gatcggtagc atctctttgg
     4501 gaatcgcctt agtggcaggc aacatatgca tcccaagacc cgctacagga ataactgctt
     4561 ttaaattcgt cattatttca tccacctgta aaatggttgc tgaattatag cttgttcgat
     4621 ttttttcgcc agcatcaatt accctgaatt gattactgaa ttacttgtga tgttacgccg
     4681 cttcgttgta gattgcagta gcattgttcc taagtatggc tccatttttc caggaatggt
     4741 cgcaaatcta ctccctccgt tccggcaatc taaagttaat cttttccaca ttaacaatat
     4801 ggtgatttat cctgtcgata tcgacggagc tttgccctct ttcattcacc gcatgaacat
     4861 ttgcaagaga tagcagtgtt tcttttttcg ccataaacac gccgcgaacg tctttgcgca
     4921 tgtcgaagtt catgctcaat gcgggtccaa cagaggattc ctgcatcact ttgatattac
     4981 gcataaaaag gtgttgcggc ttgttgtgta actccagcga cgcacgcttc atctcaacgt
     5041 tagtcagcgc cacaaaggag acagcattcc cggcggagat ttggatgccg cgcaatttgt
     5101 aagcaaggtg ggtattatcc agctgaatat tattcactcg gaaattctgc ggtatcgaga
     5161 gatatttgcc tttaattacc ccatagccga ttaacatccc ggcgctatta atcatttcaa
     5221 tattatcaat cacgaaattg tcacaaccgt aaatagcgac cgtggcgtta tcaatacctg
     5281 ctttcttact gaaatccggc gtgatattgc aggctttgat attacgaata acaaaatgtt
     5341 taccattttc aacatgtatc aactgccgac aatccgatcc cgtgatgttc gccacgacaa
     5401 agtttttcac tgcctggtct tccggatagt tgttatcata agtgcttccc gcaagaccta
     5461 tgccgatgcc ccaattgatt ttgccgttag tacagttgat gcgctcgatg acatggtcag
     5521 atatcaaaat atcacggtcg ttaatcgcca cattccattc aatggcgtcg ccctgtaagt
     5581 cgctgaactt acaattggtg atgttggcac cgataatctg gttatgaaat ccctggcgta
     5641 agatggcgta attagcgtgg ctaacggtca ggttatcgat ggtcaggtta cgcatgaccc
     5701 gtttgttttt gccgccgata taaatctgcg ttaccggacc aaagccgctc atcgccagcc
     5761 ctttgatggt gcagtcagag ccgcgcacat ccagggtgat gttatgcata ctgccgccct
     5821 cctcccctgt cacctggctg ccgtcctgta agacaaatcg ccctctgccg ttgccgcgca
     5881 ggcttccaag gatgtgtaac gttttgccag gagggataaa gatgccggtg ttgatattga
     5941 ggcaaaccaa tccggcaggc acgacgaccg tttgcccttc gctgaaggct tgtttaaatg
     6001 aggcgatcca gtcgcgaggg ttgtagtcgt taatgttaac gctttgtcgg gcgggaagcg
     6061 cgcgagcgaa aggggtatgg aggaaggcaa gcaccgagct tgccgtcagg aaggtgcgtc
     6121 gggagagttt tttaaatggc atgcgttctc ctctataaag cctgcagcaa gctggcgagt
     6181 tctcgattga tcacctgctg gttaaaatcg tgttcgactt tttcgcgcgc acgtttgacg
     6241 accggagcca gttcgtcggt gtccagttgg ctaaacgccg ccagtcgttg cgccagtgcg
     6301 cgagcatcgt tctcaggcac cagccagccg gatttatcgg cctccaccag ttccggtatt
     6361 ccgctatgca gagtagaaac caccggaatg ccgaccgcca tcgcttccat tagtgccacc
     6421 ggaatacctt ccatatcgcc atccgcacct gtaaccgatg gcaacaggaa gacatccgcg
     6481 tcgtcgagca tcgctttcac ttcgtggctc ggtttaaagc ccggcatctc taccacatct
     6541 tccagttgat attgttcgat gagggtgcgc aggcgtcgtt cccacgggcc aatgccgagg
     6601 atgcgatagc gaaatgccac gccctgctct ttcaactgac ggcaggcttc aatcgccaca
     6661 tgcaggcctt ttttctcggt taagcgtgcg acggaaatga tttccagcgg cgttgcgggc
     6721 gctttcaccg gacgcgggct aaaacgcgtc atgtccacgc ccatgcgcga tacggcgatt
     6781 ttttccctcg ggcagcccat tttttgcaac cttccggccc acagatcgct tattggtagc
     6841 atcagatcgc cacggcggaa cagttgctga tattcgggag tgtaatggtt gagcacctcc
     6901 cgactggaga tatcaatgcc gtggaagata gtggcaattt tgccgcgaat gacacccagt
     6961 tcgcggagtt ttgctgcggt tacccccgca gggccaaaat gagcgataaa gacatcggca
     7021 taaaatggtg ttgcgacctg gccgcaaatg gcagacaaaa tcaggtttcg cgactcggca
     7081 ccatagcgtt tgaggttaag tgcctgccag gtatttttac gatgaatgcc gcgtaagatc
     7141 tggctggcac ggtggcgcag tttcgccact ttgcccgtag gttcgtcctg taaccaacgg
     7201 gttctggcgg caaggttgta tttcgtccat gccgcgtggg tgttctgggt gtcgcctttt
     7261 tgcagcgcga caatctccac ctcaaagccc atatcaataa acgcggtaat ttgattgagg
     7321 acgaaggttt ccgacgacag cggaaatttc agtaaaaaga agccgacctt catttcgcct
     7381 ccctgatgtg ttcaagcaca gatttcacca tctgcattcc cgtctgacgt tcgcgactga
     7441 cggcttcgtt aagtcgcgtg ttcagtgccg gaagctggcc taaggtatcc gcaaccatca
     7501 cttgcaggct gccgtctaat aaatgacgga tatcaatcgc catctccggt agtcccagct
     7561 gctgcataat cccggcggat ttatgttcat aattgatggc aatcgccggg gtggcaaagt
     7621 tcattgagat aatggcggag tgcaggcgcg taccgatggt gagttcacag gcccccagaa
     7681 ttttgcccat ttccagatcg ttgagttcat ccatcactac gtggtaacgg gcaggatcgc
     7741 tgatgtgctg gcgcaggttg agcgccacca tgcggtcgtc tttgttatag ctgtcgatac
     7801 ccgtacaggt ggagagcgca atcacctgat agccttcgtc gagaatgcga ttgaccaccc
     7861 cggcaaaggc tttttcatat gcttgttgag tggtgccgag acgtttgtcg aacggtgcca
     7921 gttcgcgcag ggtaattgcc accgttttct gttgtgcggc aacgtccagc cagtgttgaa
     7981 cagcatagct ggcggtgaag tcttctgtgt ggtgatcgac cagccacgcg gtatcgacgc
     8041 catgttcaac ttttgaagtg gtgatattgc tgcgtttcat cagttcaagg ctgaccgatt
     8101 cgcgcaggat cagcgcgtcg cagtgaccaa aaacgtagtt cgccagttgg ttaaattgct
     8161 catcctggaa cgggccaaca ctgtgaccaa tcataaacag cgactttttc gccataaacg
     8221 tgcaaagtgc atgttcaaac tgtggcacgc cgtagagatc gacaaaaaac gatccgccga
     8281 cctgaataat ggcgtcgtaa cctgacagca ggcgcacgaa gtcggtgaat ccctgggcga
     8341 tggcgatatt acgcagcttg ccactgtcgg tgacgcgtga gagcaatacc tgatgctggt
     8401 agcggcgacg gaggactttt ttaacgcgcc cgacaacgcc cgccgcgctg ttgtgttgtt
     8461 tcatttgcag gaacagcgga tcgcccatta ccgggcggtt gagtagccag gaagaactga
     8521 caggatagcg gctcatcacg tccacttcgg catgtggatt gaggatgttg atggcatcaa
     8581 gtaagccgcg caagatggcg ctgtcgccac gattgccgca agtgtggttg cccagaataa
     8641 gtaatttcat tttttcctca taaatttgat gccaggtgag gctgtgtttg ttgtgttcgt
     8701 tgccgttttt tgcctgatgc gagcacagaa ccgtaggacg gataaggcgt tttacgctgc
     8761 acccggcaac cgttgtcgga accgaaaaca acaactcacc ccgcccgtaa aagcattttc
     8821 attttttcgc tgcgacaaaa ctgacgcttc acttccacca ccagcggatg gcgcgacagc
     8881 acaatcatca ccacaaacgc cagcacaccc gcggctattt gcaccgccag cagcatcccc
     8941 agcgccagtt gccctttcag cacaatgccc agcgcataac tgaccaccag cgttggcagc
     9001 gagagataaa acggcagcca caaactcagg atgtactggc gataactgga accaagaacc
     9061 ggtttaatca tcacgaaata gctcagaata gtgttgataa tctgcaccag caggaagcca
     9121 agcgtgacgc cgattgcgcc cgccatctgc ccaccaataa caatcgccgg aataaacaga
     9181 aatgttttaa atacgttgaa tttaaagctg atatcgaccc gcgctttcgc catcagcagc
     9241 gaaccaatcg ggttacccac ggagcgcagc agacccacca cacacagcag ctgtagcacc
     9301 ggaataatgc tgctccactt ttcgccaaat accagcggta caaagttatt cgacaccacc
     9361 atcagcccca gcagcgccgg aaagttgata atccccacta ccgacagcag cttgtagaag
     9421 ttgacgcgca gcttttcggt atcgtcctga attttggcaa acgccggaaa caacacgcgg
     9481 gtgatgatcg ggttcagttt catcggcggc acaacggcca cgttgtacgc caggttgtat
     9541 cccccagcca cgcccgcgcc gagaatacgc gccagcacga gtgttgaaag gttggtgttg
     9601 agatagttga tgatgctgtc cgccgtcagc caggcaccaa agcgtaagtt cggtgccacc
     9661 gacgccagcg agaaatgcag agcggggcga tagattttgc ggccaaagta gccaaacagc
     9721 agcgttctca ccgcactatt gaccagataa ccgaggatcg cggtcatcgc cagcggccag
     9781 aaatgggcgc taaccaccgt aaaagtaaag cccgccagca ccgcgctggt ttcgatcatg
     9841 ccgattttgt tgaactccag ctctttttgc atcaacgcgc ggaactgttg cccgtggggg
     9901 atcaccacaa acgctagtga caatgtttta atcaacggtg ccaggtccgg gttattcagc
     9961 atgtcgccaa tggcatcact caacaaaaat accgccacac acgccacgat ccccagcccg
    10021 acgttcagcc agtacaacgt ggtgagttca aggtggctga tttcttttcg ctgaataatg
    10081 gagttagcga taccgaagtc agaaagcgta tctgccagcg cgataatcac cagcgacaca
    10141 gtcagcaaac cgaactggtg gttgtcgata atccgcgcca gcacagtcat ttgcaccagc
    10201 ccgaggccga tgatgatcac cgtggcaatc gccgaccact tcgcgccgct gatggttttt
    10261 tcacgtaagc tcatatcaat atgccgcttt gttaacgaag cctttgaata ccgtcaggaa
    10321 aacgattttg atatcgaacc agacgctcca ttcgcggatg tactcaaggt cgaactcgac
    10381 gcgtttttcc attttctcca gcgtgtcggt ttcgccgcgc cagccattaa tctgcgccca
    10441 gccggtaatg cccggtttca ctttgtggcg cagcatgtag ccttcaatga gctggcgata
    10501 ctgttcgtta tgcgctactg cgtgcggacg tggaccgaca atcgacatcc ccccggtcag
    10561 cacattgata aactgcggca attcgtccag cgaggtgcgg cgcagaaagt tccccacttt
    10621 ggtgacgcgc ggatcgttct gcgtcgcctg agtcaccact ttgtcgttct ccatcacttt
    10681 catcgaacgg aacttccaca ctttgatcgg cttgccgtcc atgccgtagc gagtctggcg
    10741 gaaaataacc ggccccggtg agctcagttt caccgccaac gcaatgcagc acagcaccgg
    10801 ggagatcagc agcagaatca gcgttgccag cacaatgtct tctgcacgtt tgagcaggcg
    10861 gttaaccccg gaaagcggcg tgtcatacag cggcaccact ggtacgccgt tcatctcttc
    10921 aatacgggaa tggagaatgt tgaaggtaaa gacatcgggg atcagcagca ccgaacaggt
    10981 agtgtccgcc agttgatgga ccagtttttt cactcgcgcg ccgtcgcaca tttgcatcgc
    11041 gatatagacg ttatgaatct tgcccgcttt cgcatcctcg accagctgtt gcagattgcc
    11101 cgcccagtcg ttagaaacgc cacccggttt cgggtcgtgg taaacgccca ccacttcaaa
    11161 ccctaaccac ggctgattac ggaagctctc catcagcatt tgtccggcgg ctaaatcccc
    11221 cgctacggcg accatacgct tgttatagcc atgattacgc agccagcccg cgccaatgcg
    11281 aatacatgaa cggcaaacca ccagtccgat gctggtcagc ccataccacg ccagccagat
    11341 tttcagttgc gtgtcgaaat cattattgaa cgccaccagt ccggcgctga aaatcacgct
    11401 taaggtccag ttttgtagca acagggcaaa ttctgtcgct gcccgaacac cgcgccatga
    11461 gcgataaaaa tcggtgatgc cgcccagcat ctggaacacc accagcgtaa tcaacgccac
    11521 caacaggtgc atgtagagga atgacagtcc gctcacttcg caaaccagcc atagtccggc
    11581 aaacatgatg gtgatatctg aaaagcgttg caccatagag attaacgatg cattggtttt
    11641 cgctcgctcg cgctttttta gatttgtcat cgttgttcct gttctattgt cgcctcgccc
    11701 ctcaccccgg ccctctcccc aaaggggcga gggggagaag aaagttccgt gctcacttct
    11761 ctccgcaggc agtagagcgt cagggtgagg gggaaagtaa tgacattact cgttcagcaa
    11821 cgtcagcaga gttcgcgttc gcgcttccat cagcggtaca tcaccgcgcg attccacatt
    11881 caaccgcacc accggttcgg tgttggagga gcgcagatta aagcgccagt cggcaaaggt
    11941 catgctgatg ccatcggtgc gatccaccgc cagcgcctca cggctaaagt gctgttccac
    12001 gcggttaatc gcctcaacgg gttgcgccag tttgctgttg atctcaccgc ttgccggaaa
    12061 cgccgccatc cggtcgcgca ccagttcgcc cagcgttttt cctttcaggc acaccagttc
    12121 ggcgaccagc agccatggga tcatgccgct gtcgcagtaa gcgaaatcac ggaagtaatg
    12181 gtgggcgctc atttcgccac cgtagatggc gtcttccttg cgcatacgtt ctttaataaa
    12241 ggcgtgtccg gttttcgaca ttaccggggt gccgcctgcg gcagtcacca catcaacggt
    12301 attccaggag agacgaggat cgtggatgat cttcgcgccg ggattttttt cgaggaacgc
    12361 ttctgccagc aggccgacaa tgtagtaacc ctcaataaac tgcccttttt cgtcaaacag
    12421 gaagcagcgg tcaaaatcgc catcaaaggc aatgcccata tccgcgccgt gtttgatgac
    12481 cgcattgcgg gtgtcgtcgc ggcattccgg cagtagtggg ttaggaatac cgttggggaa
    12541 attgccgtcc ggcgtgttgt gcactttgat taattccacg ggtgcgccga gggctttaaa
    12601 gcgggcttca atggcgtcca ccaccggacc cgctgcgccg ttcccggagt tgatcaccag
    12661 cttgagcggc gtgaggtttt tgacgttgat ataaccgaac aggtgatcaa cgtaagcgtc
    12721 acgcaggttg atttgctgat agcgaccgcg tttggtttca tcgacgggag ggaagtcgtt
    12781 ggcttcagcc agacgctgga cgtcgcgcag tccggtatct ccgctgatcg ggcgagcccc
    12841 ctcgcgaacc agcttcatgc cgttataatc catcggatta tggctggcgg taacttcaat
    12901 gccgccatcc acgccgagat ggaacgtggc gaaatagatc tcttcggtgc cggacatgcc
    12961 aatatccagc acatcgacgc ccgcatcctg taaacctttc gccagcgcca gttttaaggt
    13021 ttcgctggtg aggcggacat caccgcctaa cacaatggtt ttcggtttga gaaattcgcc
    13081 ataggcgcga ccaatgcgcc aggcaatatc ttcattcagt tcttcgccta atttcccgcg
    13141 aatatcatag gctttaaagc aggttaattt tttcatatcg ttaccctttt tcaggcaata
    13201 gttggccctg accgaaacgg ccaattttgt ttttgtcgtt atttacatcg cggtttcgca
    13261 ttcattgcct gatgcgacgc tgccgcgtct tatcaggcct acaaatccga accgtaggtc
    13321 ggataaggcg ttcacgccgc atccgacaaa cagcgcctga tgcgacgttt acacccgtcc
    13381 gtagcgatcc gcgaagcgca ccacatcatc ctcttcgaga taagagccgg agcgcacttc
    13441 aattaaatcg agcggaattt tccccgggtt ttccaggcag tgcgtcgccc ccagcggaat
    13501 ataaatggac tcgttttcac caagcagttt gatatcaccg tcaatagtga cttttgccgt
    13561 tcccgcgacc accacccagt gttccgcgcg gtgatggtgc atctgtaccg acaagccttc
    13621 gcccggtttc acggtgatgc gtttcacctg gtagcggtcg cccgcgtcga tagagtcata
    13681 tttgccccac ggacgataca cttcgcgatg tacccgatgc tcatggcgac catcggcttt
    13741 gatctgctcg accacttttt tcacatcctg caccgcgtta cggtcggcaa tcagcaccgc
    13801 atctttggtc tgcactacca ccagatcttt caccccgacg gtggttacga ggccagattc
    13861 ggcatacaca tagctgtttt ctgttttgtg attaatcaca tcgccgtggc aaacgttgcc
    13921 ctcggcggtg tgggcgctga tctcccataa tgaagaccag gaaccgacat cgctccagcc
    13981 cgcatccatc ggcaccacaa cggcatctgc cgtacgttcc atgaccgcgt aatccaccga
    14041 ctcttccggg caggcgagaa acgcttcttc atccacgcga ataaaatcga gatccggatc
    14101 gacggcgctc atcgcttttt cgcaggcatc gaggatatcc gggcgatatt ttttcagttc
    14161 ttcgagatag cgtccggcgc ggaacaggaa catgccgctg ttccagtaat attcgccgct
    14221 tgccacatag gcctgggcgg tttccagatt cggtttttcg acaaactgcg ccacttcaaa
    14281 ggccaccgta tcctgctcac ccgcagacac ttcaccgcga cgaatatagc cataaccggt
    14341 ttcaggtaga tccggcacaa tgccgaaggt caccagcttg cccgcttcgg catatggcat
    14401 ggcattacgc acggcggcac ggaacgcgtc ttcatcggca atcacatgat ccgccgccaa
    14461 taccagcatt aacgggtcgc tctccgggct atgacgtttt gccgccagcg ccgccagtgc
    14521 aatggcaggt gcagtgttac gccctgtcgg ttcgagaata atgttctcgg tgagtttgtt
    14581 cagttgacgc agctgttccg cgacaataaa gcggtgctgc tcattgcaaa tcaccaccgg
    14641 gctttcgcac tccacgccgt tcaggcggca gatggtggtt tgcagcatgg tgagatcgcc
    14701 tttcaggcat aaaaactgct tggggtaaag tacgcgggaa agcggccata agcggctacc
    14761 ggagccacct gccatcacaa ctggatagag tttcgactgc gccataatta tccccgaata
    14821 tcatttataa attgacgtaa cacgttctct ttatcgagcg tgcgttcggc atattcacgt
    14881 gccaccgtgt tgtgtttggg cagcaggagc gcctgacgga tccccgccac cagcgcctcg
    14941 accgattccg gttctacgca aaccgcaatg cccgaaaagg tttcgcaaag ctcccccagt
    15001 tctgtgtggg cctctgcggt aatcaccgcg ttaccgccta ccgccagaat attggtcagt
    15061 ttcgacggca atacggcatc tgccgcgccg cgtttttgca ccaccagatg gcaatcgccc
    15121 atcttcagca gtgcgggtaa agcgtcatac gattgcagcg ggaaaaattg catgttgtgc
    15181 agtccacgct gctgcgccat tttttccagt cgcgctttgc cgccaccctg cccgacaatg
    15241 gcaaaaatca gtggttcatc gcgcaggcga tcggcagctt caataacgtt ttccagcccc
    15301 tgcttttcac caatattgcc ggagtaaaga atgatttttt tgttatccgg caggccaagc
    15361 tggttacgaa gggcatcaac atcggcatct gcaacatgct gaaaacgggc gatttccgac
    15421 cagttgggga agaagatgac gttttccgcc gccacgcctt tttcgatggc tttattcatc
    15481 atcgaacgcg aaatcgtgga gacgttatcg acgttatgta gtccgctacg ttcgaacgcc
    15541 gttgccagct gtgccacttt gccgcctttg ccttttccgg caaggcccag ccccagcatg
    15601 gcatctactt cgtaatcctg aatatgcagc acggtacgcg cgccagagag tttcgccagc
    15661 agacgcattc ccggcgtgca aaacagcgtt ggcactacgc cgataatgcg ctccggcttc
    15721 cagcgacgtt gcgccatcag cgggaaaaaa ctgctgacgg caaaactgcc gagatgcaac
    15781 aggcgtttca gggtgctcgg ctgttttggc acatacagcg ggcagcgcca caccgtggcg
    15841 gccccctctt ctcgtttgta gcgccaggcg gaatagttct cgcccacctg ccactgcggg
    15901 tagtaaggcg gtgcggtaat gacccgcacc tcatgacctt gtgccgccag ccattccacc
    15961 atctcgccgg tgtatttgcc gatgccggtt aactccggcg agtagttaat gccgtagacc
    16021 agtattttca taatccgggt actccggcac gcttctcagc gagaaaatag gcgcggctgt
    16081 tagcgtgaac attgtcactg gcgagcagcg cgtccggcgt cagccagcgg taatcgtcat
    16141 gctgctcatc cggcagtaac agatcttctt cagcgactct gaagcgaaaa ccgagcacca
    16201 catagtgagt ggtgaaatcc gtgccggaga agttatcgtc ataaaagtgc tgccagacac
    16261 cgtaaaactg gcctgctgtt atcggcagac gcagccccag ttccgccatc gtcagccgct
    16321 caaatgcggc ttccagcgtt tcgtctttct gcacgcgccc tcccggcaca aaccagtaac
    16381 cctgcgccgg gcggttggtt cttttgccaa gcagaaactc gccgcgactg ttctcgacaa
    16441 taaagtcgag agagacaagc ggtgtggagc gcactaccgt ggcaaagtct tcctgacgta
    16501 aaaacatcat tacccccgaa agcggtcttg attctcaagg aaccactggt aagtgctggc
    16561 aagccccgct tccagtgata tttcgtgata ccagccaagc tgatgcaggc gcatcacatc
    16621 cagcagtttg cgcggtgtgc catccggttt gctggcatca aaaaccaccc gacctttgta
    16681 acccaccact ttggcgatgg tttgcgccag ctcgcggata gtgcagtcaa cgcccgtgcc
    16741 gacgttaatg tgcgacagca tcggctgggt gttctccagc cagacttcgt gcgccagctc
    16801 catcacatga atgcttgccg ccgccatatc atcgacgtgc aggaattcac gcatcggtgt
    16861 accgctgccc cacaccacca cgtccggcgc attctgtgcc gtcgcctcgt ggaagcgacg
    16921 cagcaatgca gggatcacat gcgaattact cgggtggaag ttgtcgtgtg gcccgtacag
    16981 gttggtcggc atgactgaac ggtaatctcg tccgtactgg cgattgtaag attcgcacaa
    17041 tttgatcccg gcgattttgg caatagcata aggctcgtta gtcggctcca gcgtgccctg
    17101 caacaactcg ctttctgcca tcggctgttt tgccagtttc gggtagatgc aggacgatcc
    17161 gagaaacagc agtttgttca cgtcgttctg atgcgcggcg tgaatgatgt tgctctcaat
    17221 catcatgttc tggtagatga aatccgccgg ataggtgttg ttggcaacaa tgccgcccac
    17281 tttcgccgcc gccagataga cctggtcaat acgttcgctg gcaaagaaat catgcacggc
    17341 gcggctgtcc agcaggttca gctcgtcgcg ggtgcgtaat accagttcca catcaccgcg
    17401 ctgttcgagc tgtcgcttga tggcggaacc gaccatcccg cgatggccag cgataaaaat
    17461 tcgttgttta ctcatgctta tgactccagc gcgatcgcca cgtcgtagcc gtgagatttc
    17521 agcagagagt gttttttcgc cgcttcgagg tcattagcca ccatttcaga caccatctct
    17581 ctgagggtga tttccggttt ccagcccagt ttttcgtgcg ctttggtcgg gtcgccgagc
    17641 agcgtttcaa cttccgccgg acggaagtaa cgcgggtcaa cggcgataat cacatcgccc
    17701 ggtttaacgc ccggcgcgtc atgcccggtg acggaaacca caatgccctt ctcttcaacg
    17761 cccgtgcctt caaagcgcag tttgatgccc agctgtgctg ccgccatttc cacgaactga
    17821 cgtacggagt actgaacgcc ggtcgcgata acgaaatctt ctggctgttc ctgttgcagc
    17881 atcatccact gcatttttac gtagtctttg gcgtggcccc agtcacgcag ggaatccata
    17941 ttgccgaggt acaggcacga ctccagcccc tgggcgatgt tggcgattgc gcgggtgatt
    18001 ttgcgggtaa cgaaggtttc gccgcggcgc ggggattcat ggttgaagag aataccgtta
    18061 caggcgtaca tgccgtagga ttcgcggtag ttaacggtga tccagtaggc gtacagtttg
    18121 gcgaccgcat acggagatcg cgggtagaac ggcgtggttt ctttctgcgg aatttcctgc
    18181 accagaccgt acagttcaga ggtggaagcc tgatagaaac gagttttctt ttccagaccg
    18241 aggaagcgga tcgcctccag caggcgcagc gtacccatcg cgtcgacgtc agcggtatat
    18301 tctggtgact caaaagagac cgcaacgtgg ctcattgcgc ccaggttgta cacttcatcc
    18361 ggctgtactt cacgcaaaat gcgcgtcagg ttagaggtat cactcaggtc gccataatgc
    18421 agatggaatt tcgggttgca ggtgtgcgga tcctgataaa tgtgatccac gcgctcggtg
    18481 ttgaatgacg atgcgcgacg cttaatacca tgcacctcgt aacctttttc cagcagaaac
    18541 tctgccaggt aagaaccgtc ttgtccggtt acaccggtga tgagagcgac ttttgacatg
    18601 tcttattcct ctgtattttt gaatttattc agtttcaacg cgttcgcgta tcaccactgc
    18661 gggattcccc cggcaaacca catttgccgg aagcgattta aaaacactgc ttcgtgcacc
    18721 cacgacggtg ccgtcgccga ttgtgacgcc tggggcaaca aagacatcgg ttgccagcca
    18781 gcatttctcg ccaatcacaa taggcgtggc gttaatggtg aaatgttgac ttgcatggtc
    18841 gtggctaccg gtgcataaat aacttttttg cgatatcacc gaatgtgcgc caatggttat
    18901 ttcaccgagg gtatataaat tgacgtcatc gccgacccac gcgtaatcac ctaaggttaa
    18961 tttccacgga taggtaattt ttactgacgg acgaataact acgttttttc ctatttttgc
    19021 tccgaataaa cgtaataaaa aagcccgcca gcgatacaat acttgtggcg accaggcaaa
    19081 tattgttgcc tgtactgccc accataattg cactttaata gcgttgccgc cccggaaccc
    19141 tttcggcacc gagaaaccgc ttaaatcttg cattggttct ccttatattc agactttgtt
    19201 atataaggct ttcgtctttg aggtagtacg ttggcgtaaa tgccaggata attcagccca
    19261 aaagccaggt acatgtaata tttgtcgttg gacttttttc gcgtcagcac acaattccat
    19321 attattggtg gtagataccc cacccatgga aaattcagac accaggccat tgagtttttt
    19381 aaatgcataa ccggctttat acattttggc tgccagcgcg tagtcggaag aaactttata
    19441 ttccaggtca taacgccatt ttttcaagcc ggatacaggg aaaaatatcg cctgatgact
    19501 ggcgggcagg ctgtgataaa tataccagcc cggtttggcg ctacgtttaa ttttatgccc
    19561 gtcgccaaaa tccagcagcg catcgccggt gatcatcatg ttatcttttt gcatttttaa
    19621 cttacggaca aaatttgcgg catcctgatg aaaaatatcg cccgaattga gaaacaacgc
    19681 gaacttgcct tgtgccatcg taatgccttt gttcatggcg tcgtagatac cgttatctgg
    19741 ctcgctgaca aagcgtaggt tatagatacc attgagattt tccagatact cacgggtgcc
    19801 gtcattggaa ccgccatcga caacaatcca ttcgaagctg atatcttccg cctgcgccag
    19861 atgcgccagc gaggcatgtg ttttgactat cccttcgagg ttacgaaacg cgacagtgat
    19921 tatgctaagc aacatgttct tattcttacc tcgtaatatt taacgctttt cgcaaaataa
    19981 acggacagac gattaaaaat gcatattccg ggctaaatat cgaaccggta aaaaacagcg
    20041 acaccggagt aaaaagatat aattgcacgc gaaaattgcg gttatcacca aaagcgttga
    20101 tcatcatttt tatcactttc cccatgtacc acagcgataa aaacactgcg aaccaggaaa
    20161 aataaataat cagcagatac aaaccattgt ctatggtttt tccgacatcc gcaccgttaa
    20221 atattccgaa tgatgcgaca tattcataaa gtgagccaaa tctgactaca ccgtcaatat
    20281 gggtcaaaga ataaccgacc atcaccagcg gaccgacaat acgataatac gatgacgatc
    20341 cttccgtacc taaatcgccc agacgggtgg aaatataagg aaacgcgatt accacaccaa
    20401 ccaggaatac agccagagaa atcaatgcta acggtaactt tttcttaatc gcctctttat
    20461 tcagatattg aaatgcccac tccagcaaat aaaacaggat aaaggtcata acccctgaaa
    20521 acgatccgga taatattatc cctgcgagaa tcatagcatc ggttttaggc gttttgatac
    20581 caaactgttt gatgctgagc caaattgaga ttaatgccag agcgaaaaat gccggttcga
    20641 aataaagtgc tgtcgtgcgc ttgccgccga atttaatgaa attcagtaca tagctgttgc
    20701 tgtaaatcag atatttcgaa atcgactcca taatactgct gccgccggtg aggataattt
    20761 gcgccatctc caccgccgcc agcgccacca ccaacccgac caccagataa aagaaacgta
    20821 atatcttgcg atggttgtgt ggcgaaattg ttttaaaacg aatactccac accatgccaa
    20881 taatgatcac aatatagaca aacagcatag ttgaagtgac gtatttactg gcatccagcg
    20941 actgaccaaa cagatagtta aacgccgtga gtcccgcacc aatccctaag gcaatcatca
    21001 attttttaac gctgatgcgc tctaaaaaca gcagtaacaa gacaggtaaa aaagtgacga
    21061 tggtgatggg gaaactttcg ccaagctggg cgattttgac gttaaccagc agataaatca
    21121 gcggcaacaa caggtagcta cagattctga tagaagttga catactcctc cagcatctgt
    21181 tgtccactgt aggccgcgcg gctgcgttgg ctgaactcag ccagcgtggt accaaatatc
    21241 gcctgcgcga tttccggttt gcttaactgc accagttgca gcacgtcttc ttcgctgacg
    21301 gttttaccgc cggatttttg cagcacttcc cgcgccgcgt cgctatgggt ggcaatcacc
    21361 ggcacgccaa tcgatagcgc ctcacacaaa atcagcgggt agttatcgac gcgagaactg
    21421 aacaccagcg catccatctg attgagcgcg ctcatcaact tgcgcttgtc agtttcaaag
    21481 ccgtgattaa ccacgttgcc agcggtgaac ggcgagaact taccaaaggt atgcagttcg
    21541 attttgtcgc ccagcgccat catctcacgc accagttgct ggttagtttt gccgtcgtaa
    21601 cgcaggtcat gcgccaccac cgcgattttc ggcttaccct gggtttcgcg caccggaggc
    21661 agatccgcca gaatcgcttc ggttgccata tcaataccgt tattgataat ccggcaacgc
    21721 cctggaccgt acaggctatt gaaagcgtca gccacatgct ggctggggga aataaactga
    21781 cagcccagcg ccagcatctc acggaataac tggcgtttgc ccgccaccag ctggtgcgcc
    21841 cgatcaatct tcaccggcgg ataattattt aaggtcgggc atttctggca gcccgttttc
    21901 cagccttcac aaccgtcagt aaaggcgcag cgcccggtaa cgctccagtg gtcgtgcagc
    21961 gtccagacca gagtgacatc cggtttgtgg tttttcacct tttcgcaaaa gcgcaccacg
    22021 ctcttaagat ttagccagta gctgtgcagc acatgaaaat gcaggaccac cggacccggt
    22081 gtgcaagtaa tggtgcgata taactcattg aaattgccaa acagatcgcg attaaacaga
    22141 cgaaacaacg caatgttcgc catcgcggtc atccgcggcg tatgtttgac gacctgcgga
    22201 tagttttgat ggctgacgct ctctttgccg cctttgccgt agccgtagac aaaatgtgac
    22261 gccagcccct gttgcagcgc acgctgatgg agatctaacg ccacacctgc cgccccgcct
    22321 tccgccagtc gcacattaaa ttgcagaata ttcatttaat taccttcact cgcgcttttt
    22381 ctcccaccac cagcgcgttg tccgggacag agtcgagcac cacgctgccc gcgcccacgg
    22441 tgacgttgtt accaagcgtg atatcaccaa gaataatgac gttggcaccg agttcgacgc
    22501 cgttgccaat gtgtggacat gccatgttat cggcaccacg attgccgata gtgacgccgt
    22561 ggcgaatggt gaaatcatcc cccgctacca cgtttttatt gatcactacg gcgtaaccgt
    22621 gatggatagt aaagcggcgg ccaatggtcg cggcggcctg gatttcataa ccgaaaaagc
    22681 attcggtgat aatgcgatac agcaccagca gcggggccgc ccacagattg ttgaggacgt
    22741 tctttttgcg ccacaccgaa caaaaatgag caacacgata ggcaagaacc atgcagcacg
    22801 ggcgtaaact ccagctgttg gcacgcagat cttccagcat ctttagcgcc cccgaatacc
    22861 atcagccaga cgtttgccgt tacgcaccga cagcagcgtc agcaaagtgc gccaggtcat
    22921 acgtttattg cggatctggt agagggtaaa cagctgatat tttttgcttg cacggtcaaa
    22981 tttatctttg tgcttgcggt aaaagtggaa gtacccggag aattttttcg gtgacgaggt
    23041 gatctgcatt tcaccgtgat tgatatgcag gatctgcgtc gcctcttcca ctttccacgg
    23101 ttcgccgtac tccaccacca tccgcaggaa gatgtcgtaa tcctgtgctg ctttcagttc
    23161 ggtatcgaac aaacactctt tgaaacgcca tgcccaggta aagacctgat tgccaatgat
    23221 attgcgttta tagaacaggc ggcgtgagta cggcgatttg ggatacagtg gtaagcttgc
    23281 cggttgggaa taaacttctc cctggcatac gtagtcgtta gcgtacaaaa aggcgtgtgt
    23341 gaccagttgc tgtttatggg cgaggaagac gctcagacgg ttgggtgtcc attcgtcatc
    23401 gtcatcaatc ccggtgatat attccccttg cgccagcata atcgcctggt tacgtaccgc
    23461 gcacgccccg ctgttaatat cgttgtgaat gtaagtgata cgcggatcgt tgagggcggt
    23521 gacgtactgt tgcagttgtt cccaggaggt ggagcaatca tccacgatga tcatctccca
    23581 gttgctgtag tcctggcgca aaaccgattt tattgcccga atcgccagtt gttggcggtt
    23641 ccaggttggc atatagattg agatcagcgg attgtctttc ataggttgtt ctcccggctt
    23701 atgagctggt ttgcttgccg gatgcggcgt gagcgcctta tccggcctac ggggcggtgc
    23761 gaatgcaggc gtcgttattt cgcatccgac ttatattcgt attcgtaata gccataatcc
    23821 tgatacgcgc tggcgcggcg gaagatggag ttcagaatca cccctttcac cggaatacca
    23881 ttttgctcaa agcggctcag actggtttcc acttctttca atgtgttgac cgcataacgc
    23941 gccaccatta acgtggttcc aacatgacga ccaacaattg ccgcatcggt cactgccaga
    24001 atcggcggcg tatcaatcaa caccaggtca tagtttttac tcgcccagtt caccagttcg
    24061 gcaaagcgtt cgctcatcaa cagttcagaa ggatttggcg gcacctgacc gcgcgggatc
    24121 aggtcaaatt tggcaataga ggtcggttta gcagcggtag taatatcgcc ctgaccaatc
    24181 agaatttccg acaggccatt aacgttatta gtgcccaaca gctcgtgggt gtagcctttg
    24241 cgcatatcgc agtcgatcaa caacacgcgt ttattggtct ggctgatcac cgccgccagg
    24301 ttggcgcaga caaaggtttt accgattgac gggctaaccc cggtcatcat caacacattg
    24361 ttctgcgcct gcatcatcgc gaagtgcaaa ctggtacgaa gactgcggat ggcttcaatc
    24421 gccagatcgg ttggattccc caccgccagt agctggctct gtttatagcg tttaatccct
    24481 ttgatggttt tgacgctatc gcgcgctttc tgccattccg acaacgggat gctggcatag
    24541 acgctgatac cgtgttcttc cagcacctgc gggctttcga tgccgcgatt aaacaacgag
    24601 cgcagcagca cacccacgat agagagcatc aggccaagga taatcgcgcc gaggataatc
    24661 agccctttct tcggttttag cacaccaggc tgagtgattg ccgggtcaac aatgcgcaca
    24721 tcgccgacgg tgctggcttc ggtgattttc agctcctgct ctttattcag cagttgcata
    24781 tagacctgct gaccagattc gacatcgcgg gtcagacgga caatttcctg ctgggttttc
    24841 ggcatcgccg ttacgcgacc attaagtttg gctttttcgt cttccagcgc ctgacgtttc
    24901 tccagcagcg tgcggtacgc ggggtgaact ttggtgtaca gcttggagat ttccgcctct
    24961 ttaaaggtca gttcgttcaa ctgcgcgtcg atgttcacca tcgaatcgag caccgctttc
    25021 gcttccagcg gcagatcaac agaatcttta tcctgacgga aggcattcag tttgttttcg
    25081 gcaacatcga ggcggctacg tacttccggt aactgttgcg cgaggaaagc gaggcttttc
    25141 gacgcttccg ccgatttgcg ctcaatattt tgttcctgat agttacgggc gatgctgtta
    25201 agaatgtcgc ggatctgttc acgatcttca ccggtataag tcaggctcag tacgcctgcg
    25261 tctttgccgt tctccgttac cgtcaggctg ttttgcagtt gattgatcat ccccagcgtg
    25321 gagtatttgg tgacggtaaa ctcactgccc gggcgggcgt gaatggcttc aaccatcagc
    25381 gtgacgcctt cttttttcag catctggccc gcttgcccac gggcgctaaa gccgccatca
    25441 ctgctcaggg tgtagttttt attgtccagc acattaagcg taaacacctg gtccgccatc
    25501 tctttcgggc ggttaaaggt ggtcactttc accgtctcgt tctgacgtcc catcaaacga
    25561 tcccagcccg caccgaaaat cgggaacgtg tttttgctca ccgcaatatc gaggtctaga
    25621 tcgtccaccg ttttaccaag caccagacgc gaacgaatca actggatctc ggcgtccgat
    25681 gcaggcggtt tgttggctaa tgccgagcca atgtcctgca ctaacgaatt gccgctgttt
    25741 tgttcgattt gtaccagtgc gtcggcacta taaatcggcg tggcgaagaa agtgtaaacc
    25801 acagcacaga gggcaaatac agcggtgatg ccaatcaccc accagcgcgc ttcaatgacg
    25861 gtgccgacca ggcgaccaat atcgatttca tcactgcccg ttaccggagc ggcatgttgt
    25921 tttacttttt ctgtcattct tatacctgct ctgcgttcaa tgcctgcgcc cactggcggg
    25981 cagaccgttc aagtaatgtg tacaccgctg caaacgtttc ccggcttttg cgatacggat
    26041 cggggatttc acattcgtta tcccagtgac caaacagcat cactttgccg cgcatctcag
    26101 gtgccatctc gcataagcgt tcgatatggc gcttttccat ggtcaaaatc aggtcatagt
    26161 tgcgacacag gcggcggctg atttgccgtg cacagtgacc ttccagagac agttgatgtt
    26221 ctgcggcgac gctgatagcg gtaggatcag cgcccttacc gaccagcgcg ccgagtccag
    26281 cggactccac tttcagctcc gggtgataac gttgcagtaa gcgttccgcc gtcggggaac
    26341 ggcaaatatt gccgacacag acaactaaga tgttgttaaa catgacgatt accagttatg
    26401 aatgtcgctg gctgtatccg tcatgtaacg gacaccgcta atagttggca gcaactgatt
    26461 gatcagacgg ttccagcggg aaaccggggc ggtggtgaca tacaccacgt cataaggttg
    26521 caggcggaat tctgtcgcca tcaccagcga cgtcgcatcg gacatatcca gctggtagat
    26581 attggcaatc ttgccgttac gcccgccctc gcctttcaac ggacgaatga caaagatgcc
    26641 gctggcgttg gaggtggtca tgtcgatgcc ttcggcattg cccagagctt cagtcagggt
    26701 catgccgcta aagtccattt tcagggtgct ctgtttcttc acttcaccca tcacaaatac
    26761 tttcagatca tcattacgcg gcacgtagag aatatcgccg ggataaagca gtcggttctg
    26821 attgaggtcg ccgttttgca tcagcgcctg caaagaaatg cgttcttcac gaccattgtg
    26881 tgttagcacc acgttgcgcc agtcagcggt gtcggtcagg ccacctgcgg cgttgatggc
    26941 gtcgagaatg gtcagtggta cgttggtgat cgcctgttga ccggatttat tcacctgacc
    27001 tgagatatag gccttttgcg agcggaaggc ggcgatatta acgtccacct gcgggtcagc
    27061 gatgtacgtc gctaagcgcc cggtaatatc actgcggatt tcagcgagcg ttttcccgac
    27121 tacgtgaacc ttgccgatat acgggtaaaa catagtgccg tcaggctgta cccagttgcc
    27181 ggtgtcgctg gagctgcggt actgaccggc tggcgtggtg agttccgggt gatcccagac
    27241 ggtgacatta agaacgtccc ccggcccgac gcgatactga taattcgcga tctcgctttc
    27301 cagcgtcata ttggggcgcg ctacattcgg gcgtgggcgt aattggtcaa tcaggcgcgg
    27361 ggtcagcgga taaacattca ccattttgtc gagatcgaaa tcagcgtcct gctgtttgat
    27421 gacgtctttg cccatcgtcg acatattgct gcccggaagt actgtgcaac cgcttatcaa
    27481 ggttactgac accaataatg gcatcaattt cattttggat ttcatcattg tttatttatc
    27541 actttggcag agtaattatc ctgtgcacta ttaatagcaa tgtcgccatg cacatttacc
    27601 ttgcagttaa ttgaataaaa atttaactgg catcagtcct aaaaaaattg atttcatccg
    27661 caggctattg acagaataat tcagactggt ctttcaggca tccagacacg ctaccgcccc
    27721 tggcttttta gctaccaata cactgattta gcttaatttt tcaaaccctc tctgcataca
    27781 atcgttgatg agaaagggtt attgcggaaa ttaacttccg aatataaggt gacattatgg
    27841 taattgaata ttggctctcc aataatgcag gaggaagtgt tacagctaac ggaatagcag
    27901 gcaaggtaac aattcggtaa ttggctattt ttaagaatta tattaagtgt cattcaatat
    27961 ggtttttagg agtttcttta ggttgacaat atttaatatt gcgcctccac atgcgatatt
    28021 tcttaaataa tgttttatta ttaccacttt taattcaggg ataatgtgag gttattaccc
    28081 tcaaataata tgagataaat agtgctgcaa cattgcattt ttgccccgat ttatccatga
    28141 tcgaattatg acatttgtca tacaacgaat aggttttgta cttactatgg aatggattgc
    28201 cgatccgtct atctgggccg ggttaatcac gctgattgtg atcgaactgg tcctcggcat
    28261 tgataacctg gtctttattg ccatcctcgc cgaaaaacta ccgccgaagc agcgcgaccg
    28321 cgcacgggtt accgggctgc tgctggcgat gttaatgcgc ctgttactgc tggcgtcaat
    28381 ctcctggctg gtcaccctga ctcaaccgct gttcagcttc tgctcgttta cctttagcgc
    28441 ccgcgactta atcatgctgt ttggtggttt cttcctgttg ttcaaagcca cgatggagct
    28501 gaacgaacgg ctggaaggga aagacagcaa taatcccaca caacgcaaag gtgcgaagtt
    28561 ctggggcgtg gtgacgcaaa ttgtggtact ggacgccatc ttctcactcg actcagtgat
    28621 taccgccgtc gggatggtcg accatttact ggtcatgatg gccgccgtgg ttatcgctat
    28681 cagcctgatg ttgatggcca gcaagccgtt aacgcaattt gttaacagtc acccgaccat
    28741 cgttattctc tgcttaagct tcctgttgat gattggcttt agcctggtgg cagaaggttt
    28801 cggcttcgtc attccgaaag gctacctgta cgctgccatt ggtttctcgg tgatgatcga
    28861 ggcgctcaat cagttggcta tctttaaccg gcgacgtttt ctttccgcta accagacgct
    28921 gcgccagcgg actaccgaag cggtaatgcg cctgcttagc gggcaaaaag aagatgcgga
    28981 actggacgcc gaaaccgcgt ccatgctggt ggatcatggt aaccagcaga tctttaatcc
    29041 gcaggaacgg cggatgattg agcgggtact taatcttaac cagcgtactg tcagcagcat
    29101 tatgacgtcg cgccacgata ttgagcatat cgatctcaac gcgccggaag aagaaatccg
    29161 ccagttgctg gagcgaaatc agcatacgcg gctggtggtt accgacggtg atgacgcaga
    29221 agatttgctc ggtgttgttc acgttatcga cctgctacaa cagtcactgc gcggcgaacc
    29281 gctcaacctg cgggtgttga ttcgccagcc gctggtgttc ccggaaacct tgccgttgtt
    29341 acctgccctg gagcagttcc gtaatgcccg cacgcacttt gcttttgtag tggatgagtt
    29401 tggctcggtg gaagggattg tgacattaag tgacgtcact gaaaccattg ccggtaactt
    29461 accgaacgaa gtggaagaga tcgacgcccg ccatggtatt cagaagaatg cggacggttc
    29521 ctggacggcg aatggtcata tgccgctgga agatctggtg caatatgtgc cgctgccgct
    29581 ggatgaaaaa cgtgaatatc acaccattgc cgggctgttg atggaatatt tacagcgtat
    29641 tccaaaacct ggcgaagaag ttcaggtggg ggattatttg cttaaaacgt tgcaggtgga
    29701 aagccatcgc gtgcagaagg tgcagattat tccgctgcat aaggatggcg agttggagta
    29761 cgaggtgtga tggtatcggt cattttaccc gatagccgaa aggaataaga tgagggaacc
    29821 gcgccagcgg cgatttaccg ccgggagccc gggttgccag ggtggtggcg attgagccac
    29881 cctggcacgt tcaccggctt gagcgttcca tagtagtgag gaacatcaag tgaacggaac
    29941 agccttcgat cttacatatt cagaatataa tggtgccgcc gcgaacgcct gatgcgacgc
    30001 tttacgcgtc ttatcaggcc tacaggacag gtcacctggt aacctacatc ttctccagca
    30061 acttcttcac atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcaggc
    30121 gacgtttcgc ttcgtcctgt agatgtttgc gcagtaactg atccacttgc agactgtaat
    30181 tgagttgctg ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa
    30241 tcagtttgct ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg
    30301 tttgatctgc cagattcaac atgccttccc ctgtcagcgc cagcacaggc gattgacctt
    30361 gcatgtcgtt taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa
    30421 gacgcgttac gttatcgaag ttttcagcgg ccttcacatc accaccatta cgttctaccg
    30481 cttgctgaat catctgctgg aagttcatcc cttccatgcg cgtgtcggtc atttcgacat
    30541 gtgcctgtcc ttgccagttg tggcggaatg cgtcggcatc tatgtcagca ccggagaaat
    30601 caccagccag tgacattttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg
    30661 taccaatctc aacgttttcc agccgtggct ggaagtttat ccgcggattt attgatgtcg
    30721 cgtccagcgt gcccggcagt gaaacctgtc caccgttaag cttgccctgc agttgagtaa
    30781 tttccagcaa acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc
    30841 gccagcgcac gttactggcc tgcaacaaaa tatcagccgt aaagccttgc agtccctgat
    30901 atgccggttc atcaatacgc gaagaaatga ccgggcgcgg caatgttgat tggctctgcc
    30961 cctgctgcgc ggcaccgttt tcaccattcg ctgtttcatt aagcgggatg aggttgtcaa
    31021 gattcagttg cgggaattgc agtctcagct gccattcagg tttctctgtc agcgtgacct
    31081 gtgcttgccc gctcagcgtg ctgtcattgg cggtcaagct gatttggtta aatgaaaggc
    31141 gtttatgtga ctcctgccac tgggcctgga aactcccctg cccctgaata ccttgttttg
    31201 gtaaatctgc accctgcaac tgccagttaa tttgttcaat agccgccgtt aaatcatgcg
    31261 gataatcaga agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat
    31321 taacccgccc ggagaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc
    31381 ggatattgcg gattgtcacc tgctcgtcat cttcatgctg gaacaccagc acgctgtccg
    31441 ccaccttaag actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg
    31501 tattgtcgcg cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca
    31561 gttggatcac tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg
    31621 gtaaaagcgc cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg cttgcgccct
    31681 gggcggtgag agacattcgc ccggagagga tactaagctg cggccagacg tgccaacgca
    31741 gtggcccgtc gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt
    31801 cgcggaaatc attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga
    31861 ccaggagtat catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt
    31921 agtctttatc gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac
    31981 ggcggttgta aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta
    32041 acatgcccgg acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc
    32101 aaccagacca gcccggatcg atacggtgcg cggtgacgtg caccatcagc cccagacgcg
    32161 ccaaagagga acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca
    32221 gcgtcaccgc cagcgccagc tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa
    32281 cgatctcatc gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca
    32341 gatcgataaa cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgtacat
    32401 ccaccgtcgc gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt
    32461 catcaagcca ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcgc
    32521 atcactccct taacgccaat tacgtcaagg gcataccagg ttatcactga acggtacaca
    32581 atttatcaag cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg
    32641 cggtttttcc cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat
    32701 tgcaggaaca tcgggcgcac ggttttttga tattgcgcca tcacggaatc cattgaacgc
    32761 ccacgctcgt taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca
    32821 acgaaaatgg agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct
    32881 tcgagaatga tgaccttctt cggctcaacc gtcaccgttt ctttcatacg cgtatgttca
    32941 acatagctgt aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca
    33001 agcaacaggc tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc
    33061 atcgacagat ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca
    33121 tcaccgactt gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa
    33181 gccgatgcgc cagcgatacc gataatgacg cactgatgag actgatcagt catatattta
    33241 gcgacctgat taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat
    33301 agggatttca tccgcctgat accagtcgaa tagcgctgcc gcgcgctcag agttaattgt
    33361 tgacgaagaa ttcccggtgg caaattacgt tgatcagttt tatacaaggt aaaaaatgtt
    33421 atacgcagtt gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa
    33481 tgtaaaactt ttgtactagc ataaacacag aaacgaatac tggcgacctg gtcttgcgga
    33541 taaagcggta atgagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct
    33601 tcacctggtc agtttaggtt tagtctcgtt tatctttacc cttttctcgc ttgagctttc
    33661 gcagtttggc acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt
    33721 ttatcgccat gccgggcgca tgtggccggg aattgcgctg agctgctcgc tgggaaatat
    33781 cgccgcatcc atcctgcttt tttccaccag ctcgctgaac atgacctgga cgaccatcaa
    33841 tattgttgaa gccgtggtcg gggcagtgct gctacgtaaa ttgctgccgt ggtataaccc
    33901 cttgcaaaat ctggctgact ggctgcgtct ggcattcggc agcgccattg ttccgcctct
    33961 gctagggggt gttctggttg tcctgctgac gcccggagac gatcctctca gggcattttt
    34021 gatatgggta ctgtcagaat ccatcggcgc tctggcactg gtgccgctgg gattgttatt
    34081 taaaccacac tatctgctgc gccatcgcaa cccacggttg ctttttgagt cgctgctcac
    34141 gttagccatc acactgacgt taagctggct ttcgatgctg tatctgccgt ggccttttac
    34201 tttcattatt gtgctgttga tgtggagcgc cgtgcgcctg ccacgaatgg aagccttttt
    34261 gatcttcctt accacggtga tgatggtgtc gctgatgatg gccgcggatc cctccctgct
    34321 tgctacgccg cgtacatacc tgatgagcca tatgccgtgg ctaccgtttt tgctgatcct
    34381 gctgcccgcc aacatcatga cgatggtgat gtatgccttt cgtgcggaac gcaaacacat
    34441 ttccgaaagc gaaacccgtt ttcggaacgc catggaatat tccgctatcg gtatggcgtt
    34501 agtgggcacc gagggacaat ggctgcaaac caacaaagcg ctctgccagt ttctcgggta
    34561 cagtcaggaa gagctgcgcg gactcacctt tcagcaactg acctggccgg aggatctcaa
    34621 taaagatctc caacaggttg aaaagctgat aagcggtgaa ataaacacct attcaatgga
    34681 aaaacgctac tacaaccgca atggcgatgt tgtctgggcg ttgcttgccg tctcactggt
    34741 gcgccacacg gatggcacgc cgctctattt tatcgctcag attgaagaca ttaacgagct
    34801 aaaacgcacc gaacaggtga atcagcaact gatggagcgc atcacgctgg ctaacgaagc
    34861 gggcgggatt ggcatctggg agtgggagtt gaagccgaat atttttagct gggataagcg
    34921 gatgttcgag ctgtatgaaa ttcctccaca tatcaaaccg aactggcagg tgtggtacga
    34981 gtgcgtgctg ccggaagatc gccagcatgc cgaaaaagtg attcgttgca atcacgctcg
    35041 ccctttaaac tggaatttcg cattaccgta aaagacggta ttcgccatat ccgcgccctc
    35101 gccaaccggg tactgaataa agaaggcgaa gtcgaacgcc tgctcggcat taatatggat
    35161 atgactgagg ttaaacagct taacgaggca ttgtttcagg aaaaagagcg cctgcacata
    35221 acgctggact ccatcggcga agccgtggtc tgtattgata tggcaatgaa aattaccttt
    35281 atgaatccag tggcggagaa gatgagcggc tggacgcagg aagaagcatt aggtgttccg
    35341 ctcctgacgg tgttgcatat tacctttggc gacaatggtc ccctgatgga gaacatttac
    35401 agtgccgaca cctcacgttc cgcgattgaa caagatgtgg tgttgcactg ccggagcggc
    35461 ggcagctacg acgtgcatta cagtattacg ccgttaagta ctctggacgg cagcaatatt
    35521 ggttcggttc tggtgattca ggacgttacc gaatcacgca aaatgctgcg ccagctgagc
    35581 tacagcgcct cccatgatgc actgacgcat ctcgccaatc gcgccagttt tgagaagcaa
    35641 ctgcgtatgc tgctgcaaac ggtaaacagt acacatcagc gacatgccct ggtgtttatc
    35701 gatcttgatc gctttaaagc ggtgaatgac agcgccgggc atgcggcggg cgacgctttg
    35761 ctgcgcgaac tggcgtcgtt gatgctgagt atgctgcgtt ccagcgacgt gctggcgcga
    35821 ctcggtgggg atgaatttgg tctgctgcta ccagactgta atgttgaaag cgcgcgtttt
    35881 atcgctacac gcattatcag tgccgtgaat gactatcact ttatatggga aggccgtgtg
    35941 catcgggtag gtgccagtgc cgggattacc ttgattgatg acaacaatca tcaggcggca
    36001 gaagtgatgt cgcaggctga tatcgcctgt tatgcctcca aaaatggtgg acggggccgg
    36061 gtgacggttt acgaaccaca gcaagctgcc gcacatagcg aacgggcggc gatgtcgctt
    36121 gatgaacagt ggcggatgat taaagagaat cagttgatga tgatggccca cggtgtcgct
    36181 tcgccgcgga tcccggaagc gcgtaatttg tggctgattt cgcttaaact ctggagttgc
    36241 gaaggcgaga ttattgatga acaaaaattt cgtcgtagct tcagcgatcc ggcacttagc
    36301 catgctcttg accgacgggt attccacgaa tttttccagc tggccgcaaa agcggttgcc
    36361 agtaaaggca taagcatcgc cctccccctt tccgttgccg gtttgagtag cgccacgctg
    36421 gtgaatgatc tgcttgagca gctggaaaat agccctctac caccacggtt attacatctg
    36481 attattccgg cagacgcgat tttagatcac gcagaaagcg tgcaaaaact gcggctggcg
    36541 ggatgtcgga tagtgctcag ccaggtgggc cgcgatctgc aaatcttcaa ctcgctgaaa
    36601 gcgaatatgg cagattacct gctacttgat ggtgagttat gcgccaacgt gcagggtaat
    36661 ttgatggatg agatgctgat tacgattatt caggggcacg ctcagcgact cgggatgaaa
    36721 accatcgccg ggccagtcgt tttaccctta gtgatggata cgctttctgg catcggcgtc
    36781 gatctgattt atggtgatgt gattgccgat gcccaaccgc tggatttgct ggtgaatagc
    36841 agttatttcg cgattaactg agggagcgtt tgcccattgc ctgatgcgac gcatcaggcc
    36901 tacaattcgt ccgttccgtc tggttgccag ccttccgtat accagatatg caacagcgca
    36961 taagaacgcc agggcttcca gcgctcggca taacggcgga tttgtgccgg tgtcatcccc
    37021 ggaaatcgct gtttaatcag ataatcatcc ggcagaaaaa catctttcgc ctgccagcca
    37081 cgcaaagcga aataattcgc cgtccagcgc ccgatacccg gaaaagtttg cagcgttttc
    37141 atcgcctgct ccacatcgcc cggaattgtc attggtaagc tgccctccag cgccgcattt
    37201 gccagatgaa tcagtgcctc tgcccgtttc aacggcatgc ctaacgcttt taatgcctgc
    37261 gggtcggctg ctgccagccg ctgaggcgtg gggaagcaga tatactccgg aaaatcatcc
    37321 agccgttcgc cataaagctg tacaactttg gcagtcaatt ttgccgccat cgccacgctc
    37381 accagttggc ctaaaatcgc ccgcacaccc tgctcaaaag catcaataca gccgggtaaa
    37441 cgcaatccgg gccgcgccgc gcctaacttg cccaacgcac cgttaacaat ctgcgggtta
    37501 cattgcagat caaacaggcg gctcattttc gccagacact cagcggcaac gggttctaaa
    37561 cctgcactta aatttatgtg cagagtatgg cgggctatat ccggaatagc agtcaccacg
    37621 ccgcgatatt cgcccaccgc cagactacgg gcataataat cctcagcgac cgtttccaca
    37681 ccactcactg cacgggcggc gagaaatccc aacatccacg accagtcata cggcggctgc
    37741 cagttcaggg tatacatcgc atctccttat tcatccgctt tcagcataaa cgttattcag
    37801 acgctacgct ttgctttcat attccggttg tcgcgacagc aacatttcgc taaagtcacg
    37861 ccccttcttc accggcatgg ggattaattc gtgtttattg gttttgatta cggtacagca
    37921 aactgttcag tggcggtcat gcgtgacggt aaaccgcaat tactgaaaat ggaaaacgac
    37981 agcacgctgc tgccttcaat gctttgcgcg ccaacgcgtg aatcggtaag cgaatggctg
    38041 taccgccatc atgatgttcc ggcagacgac gatgaaacgc aggcgctgtt acgtcgggcg
    38101 attcgttata accgcgaaga agatatcgat gttacggcga aaagcgtgca gttcggtctt
    38161 tcctcactgg cacagtacat tgatgatcca gaagaagtgt ggtttgtgaa atcaccaaaa
    38221 tcgttcctcg gtgccagcgg cttaaaaccg cagcaggtag cgctgtttga ggatctggtc
    38281 tgcgcaatga tgttgcacat tcgccagcag gcgcaggcgc agctgccaga agcgattact
    38341 caggcggtga ttggtcgccc gatcaacttc caggggctgg gcggcgatga agcaaacgcc
    38401 caggcgcaag ggattctgga acgcgcggcg aagcgtgccg gattcaggga cgtggtattc
    38461 cagtacgagc cggtcgcggc tgggctggat tacgaagcca ccttgcagga agaaaaacgg
    38521 gtgctggtgg tggatatcgg cggtggtacg actgactgtt cattgctgct gatggggccg
    38581 cagtggcgtt cgcgtctcga tcgtgaagcc agcctgctgg gtcacagtgg ttgccgtatt
    38641 ggcggtaacg atctggacat cgcactggcg tttaaaaacc tgatgccgtt actgggcatg
    38701 ggtggcgaaa ccgaaaaagg catcgccctg ccgattctgc cgtggtggaa tgcggttgcc
    38761 atcaacgacg tacctgcgca gagtgatttc tacagtagtg ccaacggtcg tctgcttaac
    38821 gatctggtac gcgatgcccg cgaaccggaa aaagtggccc tgctacagaa agtctggcgt
    38881 gagcgtttaa gctatcgcct ggtgcgtagc gcagaagagt gcaaaattgc tctttcaagc
    38941 gtagcggaaa cccgcgcctc actgccgttt atcagcgatg aactggctac gctgattagc
    39001 cagcaagggc tggaaagcgc cctcagccag ccactggcgc ggattctgga acaggtgcaa
    39061 ctggccctgg ataacgccca ggaaaaaccg gacgttatct acctgaccgg cggtagcgcc
    39121 cgttcgccgc tgattaaaaa agcgctggca gaacagttgc cgggaattcc gattgcaggc
    39181 ggcgatgact ttggctccgt caccgccggg ctggcacgct gggcggaagt ggtgtttcgt
    39241 taaaagaaaa tggctcagca gcggcttaaa tctgcctacg cctgagccag tttttctgcc
    39301 gcctgacgta aaggttccat caacggcatg gtccgtgctg gagcatgaag gcggaatcgc
    39361 tgcaactctg tcgcgccgac agtcaacgtc gattccagcc gatgtcgttt ggcggtcatt
    39421 ttcgccatta ccgcttgtct gtctgctggc gttatcgctt cattcggcct gaacacaaaa
    39481 cggcgttcac agctcgcttt ccagtcgata accgcctggg tcagatggtc gccaaaccct
    39541 ttcacttgct taacgccacg acgggtaaca tctgcggctg tttcaatacc aaaagaccgt
    39601 aacgccgctt tacgcgcagg gccaacgccg ggaatagagg caacatcaat aaaaaatccc
    39661 tccagaaact tctgcttctg ccgttccctt gcagtgtcat gaagtgctgc cagagcgcgt
    39721 ttctcttctt ccggtaaccc gagaatttcg tccttcattt tttcgagcat cgcccgtttg
    39781 gcgataaaac cttccagccc gcccaactgt tggatctggc tgactaaatg ctcatagtcc
    39841 attttcgcgc gattaaatgc ctcacggcgt tgctggattt ctgctttgta cgctttgctt
    39901 gtcagactgc caataatcca gatagccgac agcacaggaa ccaaaataat ataacgcggt
    39961 tctgcctgaa ggccgcaaag caacagcgat aacgctgaca gtgcgatctc aatcagaatg
    40021 atgtattcgc gccgtaacag gcctaaagga agcggcctgc cagtcggttg gaaatgatcg
    40081 ggtaatggca attgcaatgc tggcggtgct actgacgcca tcaccatcgc ccagactttc
    40141 gccagcacaa aatcaccgcc ggtggtaatg acctcttcgc cgagatcaat aaaatagata
    40201 acgccttgat tatccagcgc acaccacggg cagtcggtca aatgagcggg ataaacatgc
    40261 attgccgaaa cgggacattt ctttaactgt tggcgcagag aatcaagtgc cgctacccac
    40321 gctttagccg tcggacgccc ggttgccacg ccactttccg tgaatgcctg ctgaaacatg
    40381 gcttcaacat cgcccggtaa catcgatagc ggaatcgatc gtggcggcgg ttttaaacca
    40441 cgtcgctgat tatctgacgc gtaggcataa cggaaatggg taatatccgt ctccagcgca
    40501 ttacccgcat cagagataag cggcacacca gaataaggat gccggccacc aaacaagacg
    40561 tgaaaaatca gcaacgcaag gccaaaatta tcgtgatttt ctgtgcgttc aaagccgaca
    40621 aatgacgaca aggtttgtag ctctggcggc gtaaaatgcg acacgccgac ttcgcataaa
    40681 tgcagtgtgc cattggcgtt aatctgaaag gagtcgctat caatcagtac cactttgctg
    40741 tcgcgaccta ccataaaact gttctggttt acgtcaccca cgacgtgccc gtgctcgtga
    40801 accgtagcaa aagatgaagc aatattgcgc gcaacataga gtagaaaatc ccacgcacaa
    40861 tgagggtaac gttgacgacg atgtgccggg ctatagatca tatgaatcgg ttctttacca
    40921 gaaacttttg gcatcataaa accgataact tttccgcctc gtccaccgtg aagcgttgcc
    40981 tgcggccagg cgacataatt caacaattgc gcgtcagctg tcgcagccat aaaggcaagt
    41041 ttgtcctgtt ttaaggcggg tggcggcgtg tgataaatct tggcgacgct atcgacaaac
    41101 tcattgatat cataaaccgc gccttcaccg cctttgccca gttcacggcc tggcgtaacg
    41161 cactcaccag tagcagtata taaagtgggt ttcatggtaa ccacagcgcc agtgccaacg
    41221 ttttatcatc atccgtacgc tcgtttaccg ccgggctgga caaaaactgc tttaacaatt
    41281 caggtaataa atcgagttgt tcctgcgttg ctgacgccag tccattgaaa aacggggtaa
    41341 agaaaggtac atgaggagaa ttatccagca tatttaacgc cagtcgctgg ataccatccg
    41401 taaatgctgc aactttatgc acacgctcag tgctggtgaa agtttccagt ctggaaacgg
    41461 catcttcatc ggtaatgaag tgcgtcatat tggcatattc acccaccatc ggcgtaagcg
    41521 gcaattgcag accgtgacca aaatccacca ccacgccgcc atcgccaatc tgcataatca
    41581 aggtgccatt agccgatgaa atcaatccca gaaacgtgca ggcaaaatcg cgaactgcta
    41641 attctttggc ttccgcctcg gcaaaaagtc gctgacgaac ggtcagcaca atatccgtgg
    41701 cgaggatatc attaagcccc aattccccac cctgcacttt ttgcgacata taagccatcg
    41761 cttcattgac ggcgagcgtc gcgccttctc ctccctgcga aacgctacct gcgccatcag
    41821 caaggaacat caccagtaat ggctgctggt catttagcca ggcaacctgc atctggcagg
    41881 catcctggca aggcaagtcc gcgctgatgt gcgatgttcc aacagcggat gcataaacca
    41941 gacgccagct cacactgacg tccagccttt tggcgcttcc agcacaactt ccgttcccgg
    42001 cgtggagcgg gaaaccgaac gcagcgaact ggataaccag ctaaacagtt cacggaattg
    42061 taatccttgc agaggcaaag gctgacgaac gctgatttgt gccagagtct tcatatccgc
    42121 cccctgaaca ccgatggaaa agaaagcaaa ccttttatct tcttcccccc gaaacacttt
    42181 gttggctgca gcctgccact catcggttgg tgcaccatcg gtaatcagga aaatccacgg
    42241 gcgataatac gaaataccat ttgcacgata ctcacgtttc cgctcctcta ccatatccag
    42301 ggctttggta atggccgcac ccattggtgt atcgccctgg gcaaacagga tgggcggaaa
    42361 aaaattagcg gcgctggtaa agggttgttc cacatgcacc gggccgaacg tcacaatgcc
    42421 gagttccact ctttttaaag ccagcggatc ggcaagcagt tcatcacgaa aggtcaccaa
    42481 tccggcgtta agttcattaa taggtctgcc gctcatcgag ccagaaacat ccagcagcaa
    42541 aatgcagggg caacgtggtt ccgggttact ggcaaaatcg ctggtggcaa acgtgatttg
    42601 ttcgctcata agaaatcctt tcttagagaa ttatcgaggt ccggtatcat accgactgca
    42661 ataatgaaat aagagcaaac gaagaaactt tcagaattag gcccggcggc gcagaaaatt
    42721 ttgctattcg cctcgcaaga acggagagcc gtcgattgac actaagggcg gagtgacata
    42781 atttcaggag tgagggttag ggagaggttt ccccctcccc ctggtgttct tagtaagcct
    42841 ggaagctaat cactaagagt atcaccagta tgatgacgtg cttcatcata accctttcct
    42901 tattaaaagc cctcttctcc gggagaggct ttcccgtttc agcgtcccgc tgaaatcatc
    42961 ggcttacctc ctttcgccct gaatgcagtc tatcgctaac gcgtgaataa aatagtttcc
    43021 tctgtgatta ttgcgtaacg actcgcaaag aatatttgct ggcaggatcg cagactacaa
    43081 agcctgcgga ttgacaatct tatcgtgaag gcatactttc aggagtgagg gtagagcggg
    43141 gtttcccccg ccctggtagt cttagtaagc ggggaagctt atgactaaga gcaccacgat
    43201 gatgagtagc ttcatcatga ccctttcctt atttaaggcc ccttcctcgg gaggggcttt
    43261 cccgtttcag cgtcccgctg aaatcgtcgg cttacctcct ttcgccatgc acttagtcta
    43321 tcgctgactt gcagataaaa tagtttccag tgtgattatt gcgtaacgtc tcgcaaagaa
    43381 tttttgctgg caggatcgca gacggcaaag cctgcggatt gacaatctta tcgtgaaggc
    43441 atactttcag gagtgagggt agagcggggt ttcccccgcc ctggtagtct tagtatgctg
    43501 ggtagctaat cactaagagc accacgatga tgagtagctt catcatgacc ctttccttat
    43561 ttaaggcccc ttcctcggga ggggctttcc cgtttcagca tccggctgaa atctgcggct
    43621 tacctccttt cgccctgtat gcagtctatc gctaacgcgt agataaaata gtttcctgtg
    43681 ttattactgg atgcgtgctc gcaaatgtgc ccgccattca gacgattccc gatagtgttt
    43741 cataattcct ccatttttct cccttattgg ctggctacac tagtatcatt ccgcgaaacg
    43801 tttcaggaag agaaactctt aacgatgaaa ggcagttata aatcccgttg ggtaatcgta
    43861 atcgtggtgg ttatcgccgc catcgccgca ttctggttct ggcaaggccg caatgactcc
    43921 cggagtgcag ccccaggggc gacgaaacaa gcgcagcaat cgccagcggg tggtcgccgt
    43981 ggtatgcgtt ccggcccatt agccccggtt caggcggcga ccgccgtaga acaggcagtt
    44041 ccgcgttacc tcaccgggct tggcaccatt atcgccgcta atactgttac ggtgcgcagc
    44101 cgcgtggacg gtcaactgat ggcgttacat ttccaggaag gccagcaggt caaagcaggc
    44161 gatttactgg cagaaattga ccccagccag ttcaaagttg cattagcaca aacccagggc
    44221 caactggcaa aagataaagc cacgcttgct aacgcccgcc gtgatctggc gcgttatcag
    44281 caactggcaa aaaccaatct cgtctcccgt caggagctgg atgcccaaca ggcgctggtc
    44341 agtgaaaccg aaggcaccat taaggctgat gaagcaagcg tcgccagcgc gcagttgcaa
    44401 ctcgactgga gccgcatcac cgcaccagtc gatggtcgcg ttggtctcaa gcaggttgat
    44461 gttggtaacc aaatctccag tggtgatacc accggaattg tggtgatcac ccagacgcat
    44521 cctatcgatt tagtctttac cctgccggaa agcgatatcg ctactgtagt gcaggcgcaa
    44581 aaagccggaa aaccgctggt ggtagaagcc tgggatcgca ccaattcgaa gaaattaagt
    44641 gaaggcacgc tgttaagcct cgataaccaa atcgatgcca caaccggtac gattaaagtg
    44701 aaagcacgct ttaataatca ggatgatgcg ctgtttccca atcagtttgt taacgcgcgc
    44761 atgttagtcg acaccgaaca aaacgccgta gtgatcccaa cagccgccct gcaaatgggc
    44821 aatgaaggcc attttgtctg ggtgctgaat agcgaaaaca aggtcagcaa acatctggtg
    44881 acgccgggca ttcaggacag tcagaaagtg gtgatccgtg caggtatttc tgcgggcgat
    44941 cgcgtggtga cagacggcat tgatcgcctg accgaagggg cgaaagtgga agtggtggaa
    45001 gcccagagcg ccaccactcc ggaagagaaa gccaccagcc gcgaatacgc gaaaaaagga
    45061 gcacgctcct gatgcaggtg ttacccccga gcagcacagg cggcccgtcg cgcctgttta
    45121 ttatgcgtcc tgtctccacc acgctgctga tggtggcgat cttactcgcc gggattatcg
    45181 gttatcgcgc cctgcccgtt tcggcgctgc cggaagtgga ctatccgacc attcaggtgg
    45241 tcacgctcta cccaggtgcc agcccggatg tcatgacctc tgccgttacc gcgccgctcg
    45301 aacgccagtt cgggcagatg tctggcctga aacagatgtc gtcgcaaagt tccggcggtg
    45361 cgtcagttat cactttgcag ttccagctaa cattaccgct cgatgtcgcc gagcaggaag
    45421 tgcaggccgc gattaacgct gcgaccaact tgttgccgag cgatctgcct aacccgccgg
    45481 tttacagcaa agtgaacccg gcagatccgc cgatcatgac gctcgccgtc acctcaaccg
    45541 ccatgccgat gacgcaagtg gaagatatgg tggaaacccg cgtcgcgcag aaaatctcgc
    45601 agatttccgg cgtcggcctg gtgacgcttt ccggcggtca gcgtccggct gttcgcgtca
    45661 aacttaacgc tcaggcgatt gccgccctcg gcctgaccag cgaaaccgtg cgcaccgcca
    45721 ttaccggcgc taacgttaac tcggcaaaag gtagcctcga cggcccttcc cgtgcggtca
    45781 cgctttccgc gaacgaccag atgcaatccg ccgaagagta tcgccagcta atcatcgcct
    45841 accagaacgg cgcgccaatt cgtctgggcg atgtcgcaac tgtagagcaa ggtgcagaaa
    45901 acagctggct cggcgcgtgg gcgaacaaag aacaggccat tgtgatgaat gttcagcgcc
    45961 agcccggtgc taacattatc tccaccgccg acagcattcg gcagatgctg ccacagctca
    46021 ctgagagtct gccgaaatcg gtgaaggtga cagtgctttc cgatcgcacc accaatatcc
    46081 gcgcatccgt cgatgatact cagtttgaat tgatgatggc tatcgcgctg gtagtcatga
    46141 ttatctacct gtttttgcgc aatattccgg cgaccatcat tcccggtgtt gctgtaccgc
    46201 tgtcgttaat cggcactttc gcggttatgg tgtttctcga tttttcaatc aataacctga
    46261 cactgatggc gttaactatc gccaccggat tcgtggtcga tgacgccatc gtggtgatcg
    46321 aaaacatttc ccgctatatc gaaaaaggcg aaaaaccgtt ggcggcggcg ctcaagggcg
    46381 caggtgaaat cggctttacc attatctcgc tgaccttctc actgattgcg gtgttgatcc
    46441 cactgctgtt tatgggcgat atcgtcgggc gactgttccg cgaatttgct attacgctgg
    46501 cggtagcgat tttgatctca gcggtggtgt cgctgaccct gacaccgatg atgtgcgcgc
    46561 ggatgctcag ccaggagtcg ttgcgtaaac agaaccgctt ctcccgtgcc tcggaaaaaa
    46621 tgttcgacag gataatcgcc tcctatggtc gtggactggc gaaggtgctg aatcatccgt
    46681 ggctgacctt aagcgtggcg ctcagcacgc tgctgcttag cgttctctta tgggtgttca
    46741 ttccgaaagg tttcttcccg gtacaggaca acggcattat tcagggcact ttgcaggcac
    46801 cgcaatccag ctcctttgcc aatatggccc agcgacaacg ccaggtcgcg gacgtgattt
    46861 tgcaggatcc ggcagtgcaa agcctgacct catttgttgg cgttgatggc actaacccgt
    46921 cgctgaatag tgcacgttta caaatcaacc tcaaaccgct ggatgaacgt gatgaccgag
    46981 tgcaaaaagt cattgcccgt ctgcaaacgg cggtggataa agtgccgggc gtcgatctct
    47041 tcctgcaacc aacgcaggat ctgactattg atactcaggt cagccgcacc cagtaccagt
    47101 ttaccttgca ggccacctca ctggatgcgc tcagtacctg ggtgccacag ttgatggaaa
    47161 aactccagca actgccacag ctttctgatg tctccagcga ctggcaggac aaagggctgg
    47221 tggcgtatgt caatgttgat cgcgacagcg ccagccgtct ggggatcagc atggcggatg
    47281 tcgataacgc cctgtacaac gcgtttggtc agcgactgat ttccactatt tatactcagg
    47341 ctaaccagta tcgcgtagtg ctggagcaca acaccgaaat taccccgggc ctcgcggcgc
    47401 tggataccat tcgcctgacc agtagcgacg gcggcgtggt gccgttaagc tcaattgcca
    47461 aagttgaaca gcgttttgcg ccgctttcca tcaaccatct ggatcagttc ccggtaacga
    47521 ccatctcctt taacgtgccg gataactatt cgctgggcga tgcggtgcag gcgattatgg
    47581 acaccgaaaa gacgctgaat ctgccggtgg atatcaccac gcaatttcag ggcagcaccc
    47641 tcgccttcca gtcggcgctg ggcagcactg tctggctgat tgtcgcggcg gtggtggcga
    47701 tgtatatcgt gctcggcatt ctgtacgaga gctttattca cccgatcacc attctctcga
    47761 cgctacccac cgcaggggtc ggcgcgctgc tggcattaat gattgccggt agcgaactgg
    47821 atgtgattgc gattatcggc attattttgc tgatcggtat tgtgaagaag aacgccatca
    47881 tgatgatcga cttcgcgctg gctgctgagc gcgagcaagg catgtcgccg cgcgatgcaa
    47941 tctaccaggc ttgtctgttg cgttttcgtc cgatcctgat gaccaccctg gcggctctgc
    48001 ttggcgcgct gccgctgatg ttgagtaccg gggtcggcgc ggaactgcgt cgtccgttag
    48061 gtatcggcat ggtcggcggt ctgattgtca gccaggtgct gacgctgttc accacgccgg
    48121 tgatttatct gctgttcgac cgcctggcat tgtggaccaa aagtcgcttt gctcgccatg
    48181 aagaggaggc gtaagtgaag ttttttgccc tcttcattta ccgcccggtg gcgacgattt
    48241 tactgtcggt cgccattacc ctgtgcggca tactgggctt ccgtatgctg ccggtcgccc
    48301 cgctgccgca ggtcgatttt ccggtgatta tggtcagcgc ctcgctgccc ggtgcgtcac
    48361 cagaaacaat ggcgtcttcc gttgccacgc cgctggagcg ctcacttggg cgcattgccg
    48421 gagtcagtga aatgacctcc agcagttcgc tcggcagcac gcgtattatt ttgcagtttg
    48481 attttgaccg ggatatcaac ggcgcggcgc gcgatgtgca ggcggcgatc aacgctgcac
    48541 aaagtttgct gcccagcggg atgcccagcc gtccgaccta tcgcaaagct aacccgtcgg
    48601 atgcgccaat tatgatcctc acgctgacat ccgatactta ttcgcagggt gaactgtacg
    48661 attttgcctc gacgcagctg gctccgacga tttcgcaaat cgacggtgtc ggtgatgtcg
    48721 atgttggcgg cagctcgctg cccgccgtac gcgtcgggct gaatccgcag gcgctgttta
    48781 atcagggcgt ctcgctggac gacgtacgca ccgccatcag caatgccaac gtgcgtaaac
    48841 cgcagggggc gctggaagat ggcactcacc gctggcagat ccagaccaat gatgagctaa
    48901 aaaccgccgc cgaatatcag ccgttgatta ttcattacaa caacggcggc gcggttcgtc
    48961 tgggcgatgt ggcgacggtg accgactcgg tgcaggatgt gcgcaacgcc gggatgacca
    49021 acgccaaacc ggctattttg ctgatgatcc gcaagctgcc ggaagccaat attatccaga
    49081 cggtagacag catccgggca aaattaccgg agttgcagga aaccattccg gcggcgattg
    49141 atctgcaaat tgctcaggat cgctccccca ccattcgcgc ctcgctggaa gaagtcgagc
    49201 aaacgctgat tatctcggtg gcgctggtga ttctggtggt gtttttattc ctgcgctcgg
    49261 gtcgcgccac tattattccc gccgttgcgg tgccggtttc gctgattggt acgtttgcgg
    49321 cgatgtacct gtgcggattc agtctcaata acctttcgtt aatggcgctc accatcgcta
    49381 ctggtttcgt ggtggatgac gccatcgtgg tgctggaaaa cattgcccgt catctggaag
    49441 cggggatgaa accgctacag gccgcgctac aaggtactcg cgaagtcggc tttacggtgc
    49501 tatcgatgag tctgtcactg gtggcggtgt tcctgccgct gctgttgatg ggcggattgc
    49561 cgggccgact gttacgcgaa tttgccgtga cgctttctgt cgccattgtt atatcgttgc
    49621 tggtttctct gacattaacg ccaatgatgt gtggctggat gctgaaagcc agcaagccgc
    49681 gtgagcaaaa gcgactgcgt ggttttggcc gcatgttggt agccctgcaa caaggctacg
    49741 gcaagtcgct gaaatgggtg ctcaatcata cccgtctggt gggtgtggtg ctgcttggca
    49801 ccattgcgct gaatatctgg ctgtatatct cgatcccgaa aaccttcttc ccggagcagg
    49861 acaccggcgt gctgatgggc gggatacagg cggatcagag catttcgttt caggcgatgc
    49921 gcggcaaatt gcaggatttc atgaaaatta tccgtgacga tccggcagtg gataatgtca
    49981 ccggctttac cggcggttca cgagttaaca gtgggatgat gtttatcacc ctcaagccac
    50041 gcgacgaacg cagcgaaacg gcgcagcaaa ttatcgaccg tctgcgcgta aaactggcga
    50101 aagaaccggg ggcgaatctg ttcctgatgg cggtacagga tattcgcgtt ggtgggcgtc
    50161 agtcgaacgc cagctaccag tacacgttgt tatccgacga cctggcggca ctgcgcgaat
    50221 gggagccgaa aatccgcaaa aaactggcga cgttgccgga actggcggac gtgaactccg
    50281 atcagcagga taacggcgcg gagatgaatc tggtttacga ccgtgacacc atggcacggc
    50341 tgggaatcga cgtacaagcc gccaacagtc tgttaaataa cgccttcggt cagcggcaaa
    50401 tctcgaccat ttaccagccg atgaaccagt ataaagtggt gatggaagtg gatccgcgct
    50461 atacccagga catcagtgcg ctggaaaaaa tgttcgttat caataacgaa ggcaaagcga
    50521 tcccgctgtc gtatttcgct aaatggcaac cggcgaatgc cccactatcg gtgaatcatc
    50581 agggattatc ggcggcctcg accatttcgt tcaacctgcc gaccggaaaa tcgctctcgg
    50641 acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg tgtgccttcg acggtgcgcg
    50701 gcagttttgc cggaacagcg caggtgttcc aggagacgat gaactcacag gtgatcctga
    50761 ttatcgccgc catcgccacg gtgtatatcg tgctgggtat cctttacgag agttatgtgc
    50821 acccgctgac gattctctcc accctgccct cggcgggtgt tggagcgctg ctggcgctgg
    50881 agctgttcaa tgccccgttc agcctaatcg ccctgatagg gatcatgcta ttaatcggca
    50941 tcgtgaagaa aaacgccatt atgatggtcg attttgcgct tgaagcccaa cggcacggta
    51001 acctgacgcc gcaggaagct attttccagg cctgtctgct gcgttttcgc ccgattatga
    51061 tgactaccct ggcggcgctg tttggcgcgc tgccgctggt gttgtcgggc ggcgacggct
    51121 cggagctgcg gcaacccctg gggatcacca ttgtcggcgg actggtaatg agccagctcc
    51181 ttacgctgta taccacgccg gtggtgtatc tctttttcga ccgtctgcgg ctgcgttttt
    51241 cgcgtaaacc taaacaaacg gtaaccgagt aaatgacaga tcttcccgac agcacccgtt
    51301 ggcaattgtg gattgtggct ttcggcttct ttatgcagtc gctggacacc accatcgtaa
    51361 acaccgccct tccctcaatg gcgcaaagcc tcggggaaag tccgttgcat atgcacatgg
    51421 tcattgtctc ttatgtgctg accgtggcgg tgatgctgcc cgccagcggc tggctggcgg
    51481 acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt gctgtttact ctcggttcac
    51541 tgttttgcgc gctttccggc acgctgaacg aactgttgct ggcacgcgcg ttacagggcg
    51601 ttggcggcgc gatgatggtg ccggtcggca gattgacggt gatgaaaatc gtaccgcgcg
    51661 agcaatatat ggcggcgatg acctttgtca cgttacccgg tcaggtcggt ccgctgctcg
    51721 gtccggcgct cggcggtctg ctggtggagt acgcgtcgtg gcactggatc tttttgatca
    51781 acattccggt ggggattatc ggtgcgatcg ccacattgat gttaatgccg aactacacca
    51841 tgcagacgcg gcgctttgat ctttccggct ttttattgct ggcggttggc atggcggtat
    51901 taaccctggc gctggacggc agtaaaggta caggtttatc gccgctggcg attgctggcc
    51961 tggtcgcagt tggcgtggtg gcactggtgc tttatctgct gcacgccaga aataacaacc
    52021 gcgccctgtt cagtctgaaa ctgttccgca ctcgtacctt ttcgctgggc ctggcgggga
    52081 gctttgccgg acgtattggc agtggcatgt tgccctttat gacaccggtt ttcctgcaaa
    52141 ttggcctcgg tttctcgccg tttcatgccg gactgatgat gatcccgatg gtgcttggca
    52201 gcatgggaat gaagcgaatt gtggttcagg tggtgaatcg ctttggttat cgtcgggtac
    52261 tggtagcgac cacgctgggt ctgtcgctgg tcaccctgtt gtttatgact accgctctgt
    52321 tgggctggta ctacgttttg ccgttcgtcc tgtttttaca aggaatggtc aactcaacgc
    52381 gtttctcctc catgaacacc ctgacgctga aagatctccc ggacaatctg gcgagcagcg
    52441 gaaacagcct gctgtcgatg attatgcaat tgtcgatgag tatcggcgtc accatcgccg
    52501 ggctgttgct gggacttttt ggttcgcagc atgtcagcgt cgacagcggc accacacaaa
    52561 ccgtctttat gtacacctgg cttagcatgg cgttgatcat cgcccttccg gcgttcatct
    52621 ttgccagagt gccgaacgat acgcatcaaa atgtagctat ttcgcggcga aaaaggagcg
    52681 cgcaatgaag ttctggcgac ccggtattac cggcaaactg tttctggcga ttttcgccac
    52741 ctgcattgtc ttgctgatca gtatgcactg ggcggtgcgt atcagttttg agcgtggctt
    52801 tattgattac atcaagcatg gtaatgaaca acgattacaa ctgttaagtg atgcgcttgg
    52861 cgagcagtat gcgcagcatg gcaactggcg cttcctgcgc aacaatgatc gctttgtctt
    52921 tcagatcctg cgttcatttg aacacgataa ttcggaagat aaacccggcc cgggtatgcc
    52981 accgcacggc tggcgtaccc agttctgggt ggttgatcaa aacaacaaag tgctggttgg
    53041 tccgcgagcg ccgattccac ctgacggtac acggcgaccc attctggtca acggtgcgga
    53101 agttggcgcg gtgatcgcct cccccgttga gcggttaacg cgcaatactg atatcaattt
    53161 cgataaacaa cagcggcaaa ccagctggct gatcgtcgcc ctggcaacgt tactcgcggc
    53221 gctcgccacc tttctgctgg cgcgcggttt gctggcaccg gtaaaacgac ttgtcgatgg
    53281 cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta acgcccacca gtgaagatga
    53341 actgggcaaa ctggcgcaag acttcaacca gcttgccagc acgctggaga aaaaccagca
    53401 aatgcggcgc gattttatgg ccgatatttc acacgaactg cgtacgccat tagcggtgct
    53461 gcgcggtgaa ctggaagcca ttcaagatgg cgtgcgcaaa ttcacgccgg agacggtggc
    53521 gtctttacag gcggaagtcg gtacactgac taaactggtt gacgatcttc atcagttgtc
    53581 gatgtctgat gaaggcgctc tcgcctatca aaaagcaccg gtagatttga tcccactgct
    53641 ggaagtggcg ggcggcgcat ttcgcgaacg atttgccagc cgtggcctga aactgcagtt
    53701 ttccctgcca gacagtatta ccgtatttgg cgatcgcgac cgtttaatgc agttattcaa
    53761 taacttactc gaaaacagcc tgcgctacac tgatagcggc ggcagcctga aaatctctgc
    53821 cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac agtgcgccag gtgtcagtga
    53881 cgagcagcta caaaaattat ttgaacgttt ttatcgcacc gaaggttccc gcaaccgcgc
    53941 cagcggtggt tccgggctgg ggctggcgat ttgcctgaac attgttgaag cacataatgg
    54001 ccgcattatt gctgcccatt cgccttttgg cggggtaagc attacagtag agttaccgct
    54061 ggaacgggat ttacagagag aagtatgacc gagttaccaa tcgacgaaaa cacaccgcgt
    54121 attttgatcg tggaagatga accgaagctg gggcagttgc tcattgatta tctgcgtgct
    54181 gcgagctatg cgccgacgct tatcagccac ggcgatcagg tactgccgta tgtgcgccag
    54241 acaccaccgg atctgatcct gttagatctg atgctccctg gcaccgatgg cctgacgctg
    54301 tgccgggaaa ttcgtcgttt ttctgacatt ccgatcgtga tggtgacggc aaaaatcgaa
    54361 gagatcgatc gcctgctggg gctggagatt ggcgcagatg attatatctg taagccgtac
    54421 agcccacggg aagtggtagc gcgcgtcaaa accattttgc gccgttgcaa accgcagcgc
    54481 gagttgcagc aacaggatgc tgaaagcccg ttgattatcg acgaaggtcg ttttcaggct
    54541 tcatggcgcg gtaaaatgct tgacctgacg cctgcggaat ttcgtctgct gaaaacgctc
    54601 tctcatgaac caggaaaagt gttctcccgc gagcatttgc tcaatcatct ttatgacgac
    54661 taccgcgtag taaccgaccg caccatcgac agccacatta aaaacctgcg ccgcaagctg
    54721 gaatctctcg acgccgaaca gtcatttatc cgcgccgttt acggcgttgg ttaccgctgg
    54781 gaagccgacg cctgccgcat cgtttagttt tagcgacatg actttgttag ccggagatgg
    54841 cgttcagacc agatccggca acattatccc acgcatggtc agcaaactga cattatctct
    54901 ccgccctggc aggcatcaga aaatcgctca tactttaatc ggtaaacagc acctttagat
    54961 gctgttttcg atacgcaatt tcaatcaagg agtcattatg gctggttggt ttgaactcag
    55021 taagagcagt gataatcagt tccggtttgt gctaaaagcg ggcaatggtg agactatcct
    55081 caccagcgag ctgtatacct caaaagcctc tgcggaaaag ggcatcgcgt cggtgcgtag
    55141 caacagcccg caagaagaac gctatgagaa aaaaacggca agtaacggca aattctattt
    55201 caatctgaaa gccgctaatc atcaaattat cggctccagc cagatgtacg ccaccgcgca
    55261 atctcgtgaa accggaattg cctccgttaa agccaatggc acaagccaga cggtgaaaga
    55321 caatacgtaa ttacgatgcc gggcgtgggt tgttggcgtc cggctttata tccaaataac
    55381 gcttagttat taataattaa gagtaaccgc cgccattaaa atagtgggca ctgacgagca
    55441 ctcgttgtgc tttctttgtt taccatccca tacttatcct cctgaacatg ttcatgtgcc
    55501 aaaatatctt ctaatacttt cgtgacgcta tccggtttat gacatacact gatgtaagat
    55561 ttttacttac aggcgggcgt aaaatggcca gaacgatgac ggttgatctc ggcgatgagt
    55621 tgcgtgaatt tatcgagtcc ttaattgaat ccggcgacta tcgcacccaa agcgaagtga
    55681 tccgtgagtc actgcgatta cttcgagaaa agcaagcaga gtctcgcctg caagcgttac
    55741 gtgacatgct cgcagaaggg ttgagtagtg gtgaagctca gccgtgggaa aaggacgcgt
    55801 tcttacggaa ggtgaaagca gggataagga aataatgtgc gtattattaa actcatgcca
    55861 aaagccaatg aggatttaga aggcatctgg tattacagct atcaccattt cggcgaacca
    55921 caagccgaca gatatgtgga acatctttca gacgttctcc agattctgag taacaataat
    55981 ataggtacgc ccagacctga attaggcgaa ggtatttttg ttcttccttt tgaacgccac
    56041 gtcatttatt tcctgcaatc accaggggaa attatcgtca ttcgtatcct taaccaaaac
    56101 caggacgcca ccagacattt acactggagc taaacccgcc gattgcgcta caatgcccgc
    56161 ccttaaagtg ggggcactcc cctaaccgct tcatcaggtg aagcggatct gacctgtcat
    56221 cagaacgaga gaattatgtt taaaccggaa ctcctttccc cggcgggaac gctgaaaaat
    56281 atgcgttacg ctttcgctta tggcgcagat gctgtttatg cgggccagcc gcgttattcc
    56341 ctgcgtgtgc gcaacaacga attcaaccac gaaaatcttc agctcggcat caatgaagcc
    56401 cacgcgctgg ggaaaaagtt ttatgtcgtg gtcaacattg caccgcacaa cgccaagctg
    56461 aaaaccttta tccgtgacct gaaaccggtg gtggaaatgg ggccggatgc gctgattatg
    56521 tccgatccag ggctgattat gctggtgcgt gagcacttcc ctgaaatgcc gatccacctt
    56581 tcggtgcagg ctaacgccgt gaactgggcg acggtgaaat tctggcagca aatgggcctg
    56641 acccgcgtga tcctctctcg cgagctgtcg ctggaagaga ttgaagagat ccgcaatcag
    56701 gtgccggata tggagatcga gatcttcgtt cacggcgcgc tgtgcatggc ctactccggt
    56761 cgctgcctgc tctctggcta tatcaacaag cgcgacccga accagggcac ctgcaccaac
    56821 gcctgccgct gggagtacaa cgtccaggaa gggaaagaag atgatgttgg caacatcgta
    56881 cacaagtacg agccgattcc ggtgcaaaat gttgagccga cgctgggtat cggcgcacca
    56941 accgacaaag tgtttatgat cgaagaggcc cagcgtccgg gcgagtatat gaccgcgttt
    57001 gaagatgagc acggcactta catcatgaac tcgaaagatc tgcgcgccat cgcccatgta
    57061 gaacgcctga ccaaaatggg cgtgcattcg ctgaaaatcg aaggtcgtac caaatctttc
    57121 tactattgtg cacgcaccgc acaggtttac cgcaaagcta tcgatgacgc cgctgcggga
    57181 aaaccgttcg ataccagcct gctggaaact ctggaaggtc tggcgcatcg tggctatacc
    57241 gaaggtttcc tgcgtcgtca tactcacgac gattatcaga actacgaata cggttattca
    57301 gtttctgacc gccagcagtt tgttggtgag tttaccggtg agcgcaaggg ggacctcgcg
    57361 gcggtagcgg tgaaaaataa attctccgtt ggcgacagcc ttgagctgat gacgccgcaa
    57421 ggcaacatta attttaccct tgagcacatg gaaaacgcca aaggcgaagc tatgccgata
    57481 gcaccaggcg atggttatac tgtgtggctc ccggtcccgc aggatcttga gctcaattac
    57541 gcgctgctga tgcgtaattt ctccggggaa accacgcgta atccccacgg taagtgatta
    57601 atttcgatta tttttcccgg atggaaaatt cttagaaacc gatcacatac agctgcattt
    57661 attaaggtta tcatccgttt cgctgaaaaa cataacccat aaaatgctag ctgtaccagg
    57721 aaccacctcc ttagcctgtg taatctccct tacacgggct tattttttat gcataagccc
    57781 tatccctggt caccgtcttc cattgaccac atcgatagaa tctcccttca tagcacgatg
    57841 cctttcacgt aacggcatcg tgctcgcaca ggttccggct aagcacaacc agaacgcgca
    57901 tgtttgacgc ttaccaaaaa atattctcac tctccacatt tgaatgtcag acgagcgacg
    57961 ccatgtaatc ctgcaccttc tgtcttcagg tcaactatct gcattttttt gccctgagta
    58021 acacagaaat gggctgcatc attttttact atattttctg caccagatat tctgcccctg
    58081 gctaaagaag cttcggcttc ggtgtagtat tggttatcga gtttacgctg aatattactt
    58141 ttatatgcaa gaccaaattt accgatactt gtctcatcat tatgcacagc acaaccagac
    58201 ataataaaaa tactaattaa tgatatagca gctatctttt tcatctcacc ttcccccatt
    58261 aaataccaac gacactctct agtgtttaaa tataataatg gcatgattat tataattgaa
    58321 taggattata ataaatgttc tgtacaacat ttcctacata agtaggaatt acggacattg
    58381 aggcccttca gggtaactc
//
LOCUS       DAZGJW010000014        54604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZGJW010000014 DAZGJW010000000
VERSION     DAZGJW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.014"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            318..404
                     /gene="leuL"
                     /locus_tag="ACHKIK_000896"
     CDS             318..404
                     /gene="leuL"
                     /locus_tag="ACHKIK_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO9082114.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            497..2068
                     /gene="leuA"
                     /locus_tag="ACHKIK_000897"
     CDS             497..2068
                     /gene="leuA"
                     /locus_tag="ACHKIK_000897"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO9082115.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2068..3159
                     /gene="leuB"
                     /locus_tag="ACHKIK_000898"
     CDS             2068..3159
                     /gene="leuB"
                     /locus_tag="ACHKIK_000898"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO9082116.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3162..4562
                     /gene="leuC"
                     /locus_tag="ACHKIK_000899"
     CDS             3162..4562
                     /gene="leuC"
                     /locus_tag="ACHKIK_000899"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO9082117.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4573..5178
                     /gene="leuD"
                     /locus_tag="ACHKIK_000900"
     CDS             4573..5178
                     /gene="leuD"
                     /locus_tag="ACHKIK_000900"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO9082118.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5227..6405)
                     /gene="setA"
                     /locus_tag="ACHKIK_000901"
     CDS             complement(5227..6405)
                     /gene="setA"
                     /locus_tag="ACHKIK_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFO9082119.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6507..6638)
                     /gene="sgrT"
                     /locus_tag="ACHKIK_000902"
     CDS             complement(6507..6638)
                     /gene="sgrT"
                     /locus_tag="ACHKIK_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO9082120.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWDDRNSES"
     gene            6727..8382
                     /gene="sgrR"
                     /locus_tag="ACHKIK_000903"
     CDS             6727..8382
                     /gene="sgrR"
                     /locus_tag="ACHKIK_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO9082121.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLILTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8416..8515
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8546..9529
                     /gene="thiB"
                     /locus_tag="ACHKIK_000904"
     CDS             8546..9529
                     /gene="thiB"
                     /locus_tag="ACHKIK_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO9082122.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9505..11115
                     /gene="thiP"
                     /locus_tag="ACHKIK_000905"
     CDS             9505..11115
                     /gene="thiP"
                     /locus_tag="ACHKIK_000905"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO9082123.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11099..11797
                     /gene="thiQ"
                     /locus_tag="ACHKIK_000906"
     CDS             11099..11797
                     /gene="thiQ"
                     /locus_tag="ACHKIK_000906"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO9082124.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11911..12675)
                     /gene="yabI"
                     /locus_tag="ACHKIK_000907"
     CDS             complement(11911..12675)
                     /gene="yabI"
                     /locus_tag="ACHKIK_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO9082125.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12761..13639)
                     /gene="araC"
                     /locus_tag="ACHKIK_000908"
     CDS             complement(12761..13639)
                     /gene="araC"
                     /locus_tag="ACHKIK_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO9082126.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRYPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13978..15678
                     /gene="araB"
                     /locus_tag="ACHKIK_000909"
     CDS             13978..15678
                     /gene="araB"
                     /locus_tag="ACHKIK_000909"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO9082127.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15689..17191
                     /gene="araA"
                     /locus_tag="ACHKIK_000910"
     CDS             15689..17191
                     /gene="araA"
                     /locus_tag="ACHKIK_000910"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO9082128.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17360..18055
                     /gene="araD"
                     /locus_tag="ACHKIK_000911"
     CDS             17360..18055
                     /gene="araD"
                     /locus_tag="ACHKIK_000911"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO9082129.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMEIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18130..20481
                     /gene="polB"
                     /locus_tag="ACHKIK_000912"
     CDS             18130..20481
                     /gene="polB"
                     /locus_tag="ACHKIK_000912"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO9082130.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20646..23552
                     /gene="rapA"
                     /locus_tag="ACHKIK_000913"
     CDS             20646..23552
                     /gene="rapA"
                     /locus_tag="ACHKIK_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO9082131.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23564..24223
                     /gene="rluA"
                     /locus_tag="ACHKIK_000914"
     CDS             23564..24223
                     /gene="rluA"
                     /locus_tag="ACHKIK_000914"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO9082132.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYVADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24340..25155)
                     /gene="djlA"
                     /locus_tag="ACHKIK_000915"
     CDS             complement(24340..25155)
                     /gene="djlA"
                     /locus_tag="ACHKIK_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO9082133.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25410..27764
                     /gene="lptD"
                     /locus_tag="ACHKIK_000916"
     CDS             25410..27764
                     /gene="lptD"
                     /locus_tag="ACHKIK_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO9082134.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27817..29103
                     /gene="surA"
                     /locus_tag="ACHKIK_000917"
     CDS             27817..29103
                     /gene="surA"
                     /locus_tag="ACHKIK_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO9082135.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29103..30092
                     /gene="pdxA"
                     /locus_tag="ACHKIK_000918"
     CDS             29103..30092
                     /gene="pdxA"
                     /locus_tag="ACHKIK_000918"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO9082136.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30089..30910
                     /gene="rsmA"
                     /locus_tag="ACHKIK_000919"
     CDS             30089..30910
                     /gene="rsmA"
                     /locus_tag="ACHKIK_000919"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO9082137.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30913..31290
                     /gene="apaG"
                     /locus_tag="ACHKIK_000920"
     CDS             30913..31290
                     /gene="apaG"
                     /locus_tag="ACHKIK_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO9082138.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31297..32145
                     /gene="apaH"
                     /locus_tag="ACHKIK_000921"
     CDS             31297..32145
                     /gene="apaH"
                     /locus_tag="ACHKIK_000921"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO9082139.1"
                     /translation="MATYLIGDVHGCYDELFALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            32170..32769
                     /locus_tag="ACHKIK_000922"
     CDS             32170..32769
                     /locus_tag="ACHKIK_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HFO9082140.1"
                     /translation="MADYFEIRADYNQHTITIYQAYNDAIADVAVRDGRFGAPFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQHILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGI
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            32805..33273
                     /locus_tag="ACHKIK_000923"
                     /pseudo
     CDS             32805..33273
                     /locus_tag="ACHKIK_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(33372..33551)
                     /locus_tag="ACHKIK_000924"
     CDS             complement(33372..33551)
                     /locus_tag="ACHKIK_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO9082141.1"
                     /translation="MNDKTFDFTSERDFVSRAHCVGLNLQATLQWNEVNEDAISALNE
                     FAEETGCFSDEYRKF"
     gene            complement(33572..34051)
                     /gene="folA"
                     /locus_tag="ACHKIK_000925"
     CDS             complement(33572..34051)
                     /gene="folA"
                     /locus_tag="ACHKIK_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO9082142.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34243..36105)
                     /gene="kefC"
                     /locus_tag="ACHKIK_000926"
     CDS             complement(34243..36105)
                     /gene="kefC"
                     /locus_tag="ACHKIK_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO9082143.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36098..36628)
                     /gene="kefF"
                     /locus_tag="ACHKIK_000927"
     CDS             complement(36098..36628)
                     /gene="kefF"
                     /locus_tag="ACHKIK_000927"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO9082144.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(36736..38067)
                     /gene="yaaU"
                     /locus_tag="ACHKIK_000928"
     CDS             complement(36736..38067)
                     /gene="yaaU"
                     /locus_tag="ACHKIK_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9082145.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38126..38413)
                     /gene="fixX"
                     /locus_tag="ACHKIK_000929"
     CDS             complement(38126..38413)
                     /gene="fixX"
                     /locus_tag="ACHKIK_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO9082146.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38410..39696)
                     /gene="fixC"
                     /locus_tag="ACHKIK_000930"
     CDS             complement(38410..39696)
                     /gene="fixC"
                     /locus_tag="ACHKIK_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO9082147.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(39747..40688)
                     /gene="fixB"
                     /locus_tag="ACHKIK_000931"
     CDS             complement(39747..40688)
                     /gene="fixB"
                     /locus_tag="ACHKIK_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO9082148.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(40703..41473)
                     /gene="fixA"
                     /locus_tag="ACHKIK_000932"
     CDS             complement(40703..41473)
                     /gene="fixA"
                     /locus_tag="ACHKIK_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO9082149.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            41947..43461
                     /gene="caiT"
                     /locus_tag="ACHKIK_000933"
     CDS             41947..43461
                     /gene="caiT"
                     /locus_tag="ACHKIK_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO9082150.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            43492..44634
                     /gene="caiA"
                     /locus_tag="ACHKIK_000934"
     CDS             43492..44634
                     /gene="caiA"
                     /locus_tag="ACHKIK_000934"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO9082151.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            44763..45980
                     /gene="caiB"
                     /locus_tag="ACHKIK_000935"
     CDS             44763..45980
                     /gene="caiB"
                     /locus_tag="ACHKIK_000935"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO9082152.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            46054..47607
                     /gene="caiC"
                     /locus_tag="ACHKIK_000936"
     CDS             46054..47607
                     /gene="caiC"
                     /locus_tag="ACHKIK_000936"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO9082153.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLLTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            47716..48501
                     /gene="caiD"
                     /locus_tag="ACHKIK_000937"
     CDS             47716..48501
                     /gene="caiD"
                     /locus_tag="ACHKIK_000937"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO9082154.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            48507..49097
                     /gene="caiE"
                     /locus_tag="ACHKIK_000938"
     CDS             48507..49097
                     /gene="caiE"
                     /locus_tag="ACHKIK_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO9082155.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(49183..49578)
                     /gene="caiF"
                     /locus_tag="ACHKIK_000939"
     CDS             complement(49183..49578)
                     /gene="caiF"
                     /locus_tag="ACHKIK_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO9082156.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(49839..53060)
                     /gene="carB"
                     /locus_tag="ACHKIK_000940"
     CDS             complement(49839..53060)
                     /gene="carB"
                     /locus_tag="ACHKIK_000940"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO9082157.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(53078..54226)
                     /gene="carA"
                     /locus_tag="ACHKIK_000941"
     CDS             complement(53078..54226)
                     /gene="carA"
                     /locus_tag="ACHKIK_000941"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO9082158.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT        13192 a        14761 c        14259 g        12392 t
ORIGIN      
        1 caaatcaata tcaaaaaaaa tcgcaaaaca tataattcaa tacaaatcat caggataggt
       61 tttgcaacgc gtgcattttg tccccttttt cctcgttgat tagatgcaaa aatttatgct
      121 gaaatatgtc aaccgatgaa aagcgtcggt agttaagcag aaattaatat cgcttacttt
      181 aaccaccgca gcacaattag ctaattttac gggtgcagaa ctcacgctgg cgggacgttt
      241 ttattgcgtc agggttgaca tccgtttttg tatccagtaa ctctaaaagc atatcgcatt
      301 catctggagc tgatttaatg actcacatcg ttcgctttat cggtctacta ctactaaacg
      361 catcttcttt gcgcggtaga cgagtgagcg gcatccagca ttaagccagc acgcagtcaa
      421 acaaaaaacc cgcgccattg cgcgggtttt tttatgcccg aagcgaggcg ctctaaaaga
      481 gacaaggacc caaaccatga gccagcaagt cattattttc gataccacat tgcgcgacgg
      541 tgaacaggcg ttacaggcaa gcttgagtgt gaaagaaaaa ctgcaaattg cgctggccct
      601 tgagcgtatg ggtgttgacg tgatggaagt cggtttcccc gtctcttcgc cgggcgattt
      661 tgaatcggtg caaaccatcg cccgccaggt taaaaacagc cgcgtatgtg cgttagctcg
      721 ctgcgtggaa aaagatatcg acgtggcggc cgaatccctg aaagtcgccg aagccttccg
      781 tattcatacc tttattgcta cctccccaat gcacatcgcc accaagctgc gcagcaccct
      841 ggacgaggtg atcgaacgcg ctatctatat ggtgaaacgc gcccgtaatt acaccgatga
      901 tgttgaattt tcttgcgaag atgccgggcg tacacccatt gccgatctgg cgcgagtggt
      961 cgaagcggcg attaatgccg gtgccaccac catcaacatt ccggacaccg tgggctacac
     1021 catgccgttt gagttcgccg gaatcatcag cggcctgtat gaacgcgtac ctaacatcga
     1081 caaagccatt atttccgtac atacccacga cgatttaggc ctggcggtcg gcaactcact
     1141 ggcggcggta catgccggtg cacgccaggt ggaaggtgca atgaacggga tcggcgagcg
     1201 tgccggtaac tgttcgctgg aagaagtcat catggcgatt aaagttcgta aggatattct
     1261 caacgtccac accgccatta atcaccagga aatatggcgc accagccagt tagttagcca
     1321 gatttgtaat atgccgatcc cggcaaacaa agccattgtt ggcagcggcg cattcgcaca
     1381 ctcctccggt atccaccagg atggcgtgct gaaaaaccgc gaaaactacg aaatcatgac
     1441 accagaatct attggtctga accaaatcca gctgaatctg acctctcgtt cggggcgtgc
     1501 ggcggtgaaa catcgcatgg acgagatggg gtataaagaa agtgaatata atttagacaa
     1561 tttgtacgac gccttcctga agctggcgga caaaaaaggt caggtgtttg attacgatct
     1621 ggaggcgctg gccttcatcg gtaagcagca agaagagccg gagcatttcc gtctggatta
     1681 cttcagcgtg cagtctggct ctaacgatat cgccaccgcc gccgtcaaac tggcctgtgg
     1741 cgaagaagtc aaagcagaag ccgccaacgg taacggtccg gtcgatgccg tctatcaggc
     1801 aattaaccgc atcactgact ataacgtcga actggtgaaa tacagcctga ccgccaaagg
     1861 tcacggtaaa gatgcgctgg gtcaggtgga tattgtcgcc aactacaacg gtcgccgctt
     1921 ccacggcgtc ggcctggcca ccgatattgt cgagtcctcc gccaaagcca tggtgcacgt
     1981 actgaacaat atctggcgtg ccgcagaagt cgaaaaagag ttgcaacgca aagctcaaca
     2041 caacgaaaac aacaaggaaa ccgtgtgatg tcgaagaatt accatattgc cgtattgccg
     2101 ggggacggta ttggtccgga agtgatgacc caggcgctga aagtgctgga tgccgtgcgc
     2161 aaccgctttg cgatgcgcat caccaccagc cattacgatg taggcggtgc agccattgat
     2221 aaccacgggc aaccactgcc gcctgcgacg gttgaaggtt gtgagcaagc cgatgccgtg
     2281 ctgtttggct cggtaggcgg tccgaaatgg gaacatttac caccagacca gcaaccagaa
     2341 cgcggcgcgc tgttgccttt gcgtaagcac ttcaaattat tcagcaacct gcgcccggca
     2401 aaactgtatc aggggctgga agcattctgt ccgctgcgtg cagacattgc cgctaacggc
     2461 ttcgacatcc tgtgcgtgcg cgaactgacc ggcggcatct atttcggtca gccaaaaggc
     2521 cgcgaaggta gcggacaata tgaaaaagcg tttgataccg aggtgtatca ccgttttgag
     2581 atcgagcgta ttgcccgcat cgcgtttgaa tctgctcgta aacgtcgcca caaagtcacc
     2641 tcaatcgaca aagctaacgt gctgcaatcc tctattttat ggcgtgaaat cgtcaacgag
     2701 atcgccacgg aatacccgga tgtcgaactg gcgcatatgt acatcgacaa cgccaccatg
     2761 cagctgatta aagatccatc acagtttgac gttctgctgt gctccaacct gtttggcgac
     2821 attctgtctg acgagtgcgc aatgatcact ggctcgatgg ggatgttgcc ttccgccagc
     2881 ctgaacgagc aaggttttgg tctgtatgaa ccggcaggcg gctcagcacc agatatcgca
     2941 ggcaaaaaca tcgccaaccc gattgcgcaa attctgtcgc tggcactgct gctgcgctac
     3001 agcctggatg ccgatgatgc ggcttgcgcc attgaacgcg ccattaaccg cgcattagaa
     3061 gaaggcattc gcaccgggga tttagcccgt ggcgctgccg ccgttagtac cgatgaaatg
     3121 ggcgatatca ttgcccgcta tgtggcagaa ggggtgtaat catggctaaa acgttatacg
     3181 aaaaattatt cgacgctcac gtagtgtacg aagccgaaaa cgaaaccccg ctgttatata
     3241 tcgaccgcca cctggtgcat gaagtgacct caccgcaggc gttcgatggt ctgcgcgccc
     3301 acggtcgccc ggtacgtcag ccgggcaaaa ccttcgccac tatggatcac aacgtctcta
     3361 cccagacgaa agacattaat gcctgtggtg aaatggcgcg catccagatg caggagctga
     3421 ttaaaaactg caaagaattt ggcgtcgaac tgtatgacct gaatcacccg tatcagggga
     3481 tcgtccacgt aatggggccg gaacagggcg tcaccttgcc ggggatgacc attgtctgcg
     3541 gcgactcgca taccgccacc cacggcgcgt ttggcgcact ggcctttggt atcggcactt
     3601 ccgaagttga acacgtactg gcaacgcaaa ccctgaaaca gggccgcgca aaaaccatga
     3661 aaattgaagt ccagggcaaa gccgcgccgg gcattaccgc aaaagatatc gtgctggcaa
     3721 ttatcggtaa aaccggtagc gcaggcggca ccgggcatgt ggtggagttt tgcggcgaag
     3781 caatccgtga tttaagcatg gaaggtcgta tgaccctgtg caatatggca atcgaaatgg
     3841 gcgcaaaagc cggtctggtt gcaccggacg aaaccacctt taactatgtc aaaggccgtt
     3901 tgcatgcgcc gaaaggcaaa gatttcgacg acgccgttgc ctactggaaa accctgcaaa
     3961 ccgacgaagg cgcaactttc gataccgttg tcactctgca agcagaagaa atttcaccgc
     4021 aggtcacctg gggcaccaat ccaggccagg tgatttccgt gaacgacaat attcccgatc
     4081 cggcttcgtt tgccgatccg gttgaacgcg cgtcggcaga aaaagcgctg gcctatatgg
     4141 ggctgaaacc gggcattccg ctgaccgaag tggctatcga caaagtgttt atcggttcct
     4201 gtaccaactc gcgcattgaa gatttacgcg cggcagcgga gatcgccaaa gggcgaaaag
     4261 tcgcgccagg cgtgcaggca ctggtggttc ccggctctgg cccggtaaaa gcccaggcgg
     4321 aagcggaagg tctggataaa atctttattg aagccggttt tgaatggcgc ttgcctggct
     4381 gctcaatgtg tctggcgatg aacaacgacc gtctgaatcc gggcgaacgt tgtgcatcca
     4441 ccagcaaccg taactttgaa ggccgccagg ggcgcggcgg gcgcacgcat ctggtcagcc
     4501 cggcaatggc tgccgctgct gctgtgaccg gacatttcgc cgacattcgc aacattaaat
     4561 aaggagcaca ccatggcaga gaaatttatc aaacacacag gcctggtggt tccgctggat
     4621 gccgccaatg tcgataccga tgcaatcatc ccgaaacagt ttttgcagaa agtgacccgt
     4681 acgggttttg gcgcgcatct gtttaacgac tggcgttttc tcgatgaaaa aggccaacag
     4741 ccaaacccgg acttcgtgct gaacttcccg cagtatcagg gcgcttccat tttgctggca
     4801 cgagaaaact tcggctgtgg ctcctcgcgt gagcacgcgc cctgggcatt gaccgactac
     4861 ggttttaaag tggtgattgc gccgagtttt gctgacatct tctacggcaa tagctttaac
     4921 aaccagctgc tgccggtgaa attaagcgat gcagaagtgg acgaactgtt tgcgctggtg
     4981 aaagccaatc cggggatcca tttcgacgtg gatctggaag cgcaagaggt gaaagcggga
     5041 gagaaaacct atcgctttac catcgatgcc ttccgccgcc actgcatgat gaacggtctg
     5101 gacagtattg ggcttacctt gcagcacgac gacgccattg ccgcttatga agcaaaacaa
     5161 cctgcgttta tgaattaatc cccttgcccg gtcaaatgac cgggctttcc gctatcgtcc
     5221 acgtcatcaa acgtctttaa cctttgcggt taaaaataat gcgacaacag aaataaccgc
     5281 aattacccag tagacagcaa agtgccccca actttgtgca attgctccct gaataacgcc
     5341 agccagaatt accccggtag aaatactgtt agtaaataag gtggtagctg cccccgctct
     5401 tccaggcatt aaatcctgaa accatagcat cccaataccc gcaacaatgc cgataaatac
     5461 agcgttaaaa agttgcagcg tcatcaacgc catacggcta tgaaagaaaa tcaatccggt
     5521 gtaaaacagt actcctgccg ccactgctat gaccatcatt cgccgcttac cataacgttt
     5581 gacatagtag ccagccagaa tcattgctgg tatttccagt ccagctgccg tccccatcag
     5641 gaaacccgcc agtttgtctg gcaatcctaa ctcgctactg atccacaacg gcatatcaat
     5701 aatgtacatg gtgttgcagg tccacattaa cgtcgaggcg acaaataaca tccgtacgtt
     5761 actatcctgc cagccacctt gcattgataa agcattttcc gacggcagtt ctacccgcgc
     5821 cacagacgga agcataaatg caatcaatac cagactgagt gtgaatatcc cggcggcaat
     5881 cgaaaacatc accgtaaagc cgtaattcaa cgccagcata aaggccaacg gtggaccgat
     5941 aacccatgcc agagaaagct gcgcacgcat caccgagcta aacatcacca cttctcgcgc
     6001 cgagttatcc gcatattccc gcgccagagc aaataactgt ggcattgccg tattggccag
     6061 agatgccaga agcacaccac aggtgataag cgtcagataa tgacgattaa atgcaaacaa
     6121 tagcgcattg ccgatagcca tcaaacagca aaatataatc agttttcgcc gatcgccctg
     6181 actgtcagaa cgttttgcca accagaggct tacgccgatc ccagcaatag cattcaccgt
     6241 ataaaagagt ccgatccaga aaggttgcgc gccaacctca cgactcagaa ataagctcaa
     6301 tgtaggagcc tgtagcgccc cggccacccc catcataaaa gcgaccagca taaatgccgc
     6361 gtaaacaccg ttcatacggc gagccatcgt cattatccag atcatacgtt ccctttttag
     6421 cgcggcgaga ataaaaaaaa ccagcaggta taatctgctg gcgggtgatt ttacaccaat
     6481 actcagtcac acatgatgca ggcaagtcaa ctttcagaat tgcggtcatc ccactgcatc
     6541 agttcttcaa gcatggttaa tcgttgtggg acgcttaacc aacgcaacca gcacaacttc
     6601 gctgtcgcgg taaaatagtg ctgataaaac tgacgcatgg ggcacctcct tgcttcatcg
     6661 ttaggaatta ttgacttaat atagggaaaa taaaattgct gtcttttgcg caggagttcc
     6721 ccttttatgc catctgctcg tctgcaacaa cagttcatcc gcctgtggca atgctgcgag
     6781 ggtaaatcgc aggacacaac gctcaacgaa ctggcagcgt tattgagctg ctcgcgtcgt
     6841 catatgcgta ccctgctcaa caccatgcag gatcgcggct ggctgacgtg ggaagcggaa
     6901 gtcgggcgcg gtaaacgctc gcgtctgaca ttcctctata ccgggctggc gcttcagcaa
     6961 cagcgggcgg aagacctgct ggagcaggat cgtatcgacc aactagtgca gttggttggc
     7021 gacaaagcga ctgtgcggca aatgctggtt tctcatctgg gccgcagctt ccgccagggg
     7081 cggcacatcc tgcgcgtgct ctactatcgt ccgttgcgta atctgctacc tggcagcgca
     7141 ttgcgccgtt ccgaaaccca tattgcccgg caaatcttca gttcgctaac gcgcataaat
     7201 gaggaaaatg gggaactgga agcagacatc gcccaccact ggcagcaaat atcaccgctt
     7261 cactggcgtt tctttttgcg tccaggagtc cattttcatc atggtcgtga actggaaatg
     7321 gacgacgtga tcgcctcttt aaaacgaatc aatacgctgc cgctctattc gcatattgcg
     7381 gacattgtgt cgccgacgcc ctggacgctg gatatccatc tcacgcaacc ggaccgctgg
     7441 ttaccgttac tgctgggaca agttccggcg atgatcctgc cgcgcgaatg ggaaaccctc
     7501 agtaactttg ccagccatcc catcggcacc ggtccgtatg cggtgatccg caacaccacc
     7561 aatcaactga aaattcaggc attcgatgac ttcttcggtt accgggcatt aatcgatgaa
     7621 gttaacgtct gggttctgcc ggaaattgcc gacgagccag ctggaggtct gatgctaaaa
     7681 ggtccacagg gtgaggaaaa agagattgaa agccgcctgg aggaaggttg ctactattta
     7741 ctgttcgaca gccgcaccca tcgcggggcg aatcagcaag tcagggactg ggtaagctat
     7801 gtgctttctc caactaatct ggtctatttc gctgaggaac agtaccagca actgtggttc
     7861 ccggcttatg gactgctccc ccgttggcac catgcccgca ccataaagag cgaaaaaccg
     7921 gctggcctgg aaagcctcat cctgaccttt tatcaggatc acagtgagca tcgggtgatt
     7981 gccgggatca tgcagcagat tctggcaagt caccaggtca cgctggaaat caaagagatc
     8041 agctacgatc agtggcatga aggagagatc gagagcgata tctggcttaa cagcgccaac
     8101 tttacgctgc ctctggattt ttcgctgttc gcgcacttgt gcgaggtgcc gctgctccaa
     8161 cactgtattc cgatcgactg gcaagccgac gccgcccgct ggcgcaatgg cgaaatgaac
     8221 ctggcgaact ggtgccagca actggtcgcc agcaaagcaa tggtgccgct tatccaccac
     8281 tggctgatca ttcaggggca acgcagtatg cgcggcctgc gcatgaacac cctcggctgg
     8341 ttcgatttta aatcagcgtg gtttgcgcca ccggatccat gattgctggt agataacaaa
     8401 atcactacac taacgccgtt ctcaacgggg tgccacgcaa acgcgtgcgc tgagaaaata
     8461 cccgtcgaac ctgatccgga taacgccggc gaagggattt gaggctcctt ctcaagtcct
     8521 ttgccactct tttttgaggt gcaaagtgtt aaaaaaatgt cttcccctgc tgttgctgtg
     8581 cacagcgccc gttttcgcta aacccgttct gactgtttat acctacgatt ccttcgccgc
     8641 cgactggggg cctggtccaa agatcaaaaa agcttttgaa gccgactgta actgcgaact
     8701 gaaactggtg gcgctggaag atggcgtttc gcttctcaac cgtctacgga tggaaggcaa
     8761 aaatagtaaa gccgatgtgg tgctggggct ggataacaat ctgttagacg ccgccagtaa
     8821 aaccggacta tttgccaaaa gcggtgtggc agcggatgcc gttaacgttc ccggcggctg
     8881 gaataatgac actttcgtcc cgtttgatta cggctatttc gccttcgtct atgacaagaa
     8941 caaactgaaa aacccgccgc aaagcctgaa agaattggtt gagagcgatc agaactggcg
     9001 ggtgatttat caggatccgc gcaccagtac gccggggctg ggcctgctgt tgtggatgca
     9061 aaaagtctat ggcgatgacg ccccacaagc ctggcaaaaa ctggcgaaga aaaccgtcac
     9121 ggtcaccaaa ggctggagcg aagcctacgg cctgttttta aaaggtgaaa gcgatctggt
     9181 actgagttac accacctctc cggcttatca cattctcgaa gagaaaaaag ataactacgc
     9241 cgccgcgaac tttagcgaag gtcactatct gcaggtggaa gtcgccgccc gcaccgctgc
     9301 cagcaagcag ccggagctgg cgcaaaaatt cctccagttt atggtttctc cggcttttca
     9361 gaatgcgatc ccaaccggta actggatgta tccggtggca aatgtcacgc tgcctgccgg
     9421 gtttgaacaa ttgaccaaac cggccaccac gctggagttc acaccagctg aagtggctgc
     9481 acaacgtcag gcatggatta gcgaatggca acgcgccgtc agccgttaat tcccggctgg
     9541 ttaattccag gtgtaagcgc cgccacgctg gtggtagcgg ttgcgctcgc ggcgtttctc
     9601 gccctgtggt ggaacgcgcc gcagggtaac tgggtggcag tctggcagga cagctacctg
     9661 tggcatgtgg tgcgcttctc cttctggcag gcgtttctct cggcgctact ctctgtcgta
     9721 cccgcgatat tcctcgcccg cgccctctat cgcaggcgct ttccgggtcg gctggcgctg
     9781 ttgcgtctgt gcgcaatgac cttgatcctc ccggtgctag ttgctgtttt cggcattctt
     9841 agcgtctatg gtcgtcaggg ctggctggca tcgctctgcc aatcgctcgg tctggagtgg
     9901 accttttcgc cctacggcct gcaaggcatt ttgctggcgc acgtattttt taatctgccg
     9961 atggcgagcc gcttattact ccaggcactg gaaaacattc ccggcgaaca acgtcagctt
    10021 gccgcccagc ttgggatgcg cggctggcat tttttccgct tcgtcgaatg gccgtggcta
    10081 cggcgacaaa tcccgccggt tgccgcgctt atctttatgc tctgtttcgc cagcttcgcc
    10141 accgtgctat cgctgggcgg cggtccgcag gcaactacta tcgagctggc tatttatcag
    10201 gcgctgagtt acgactacga tcctgcccgc gcggcgatgc tggcgctgct ccagatggtg
    10261 tgttgccttg ggctggtgct gttgagtcag cgactaagta aggccattgc gcccggtacc
    10321 acgctgctgc aaggctggcg cgatccggac gatcgtctgc atagccgcat ttgcgacacg
    10381 gtgttaatag tgctggcgct gctgctgttg ctgccaccgt tgctggcggt gatcgtcgat
    10441 ggggtaaatc gccagttgcc ggaagtgctg gcacaaccgg tgctgtggca ggcgctgtgg
    10501 acctcgttgc gtattgcgct ggcggcaggt gtattgtgcg tagtgctgac catgatgctg
    10561 ctatggagca gtcgcgaact gcgggcgcgg cagaaaatgc tggcgggtca ggcgctggag
    10621 atgagcggca tgttgatcct cgccatgccg gggattgtgc tggctaccgg cttcttttta
    10681 ctgctcaaca acactatcgg cctgccacaa tctgctgacg gcattgtgat tttcaccaat
    10741 gcgttaatgg cgatccctta tgcgctgaaa gtactggaaa acccgatgcg cgatatcacc
    10801 gcccgctaca gtatgttgtg tcagtcgctg ggcattgaag gctggtcgcg cttaaaagtg
    10861 gtcgagctgc gcgccctgaa acgtccactg gcgcaggcgc tggcttttgc ctgcgtgctg
    10921 tcgattggtg attttggcgt agtggcgttg ttcggtaacg atgatttccg taccctgccg
    10981 ttttatctct accagcaaat tggctcctat cgcagccagg acggcgcggt caccgcgtta
    11041 attctgctac tactctgttt tctgctgttt accgtgattg aaaaactacc ggggcgaaat
    11101 gttaaaactg actgatatca cctggcttta tcaccatttg ccgatgcgtt ttagcttaac
    11161 ggtagaacgc ggcgagcagg tggcaatcct cgggccaagc ggcgcgggta aaagtaccct
    11221 gctgaatttg attgccggtt ttctgacgcc agccagcggt tcgctgacta tcgacagcgt
    11281 tgatcacacc acgacgccgc cgtcacgccg cccggtatcg atgctgtttc aggagaacaa
    11341 cctgttcagc cacctgacgg tcgcgcagaa catcgggctg gggcttaatc cgggattgaa
    11401 gctgaacgcg gcgcagcagg agaaaatgca cgctatcgcc cgccagatgg ggattgataa
    11461 tttaatggcg cggttaccgg gcgagctttc cggcggtcag cgacagcgcg tggcgttagc
    11521 gcgttgtctg gtgcgcgaac agccgatttt attgctcgat gaaccgttct ctgcgctcga
    11581 tccggcgtta cgtcaggaga tgctgacgct ggtgagcacg agctgccagc agcaaaaaat
    11641 gacgctattg atggtgtcgc acagtgtgga agatgcggcg cggatcgcca cacgctcggt
    11701 ggtggtcgcc gacgggcgca tcgcctggca gggtaagacc aatgagttgt tgagcggtaa
    11761 ggcgagtgct tcggcactat tggggattac gggttagtat gcactgtcgg atgcggcgtg
    11821 aacgccttat ccgacctacg ggtagtgcgt tttgtaggcc tgataagacg cggtcagcgt
    11881 cgcatcaggc aacccgcaca agactatctc ctaaccccca accactttac gcaaaatatc
    11941 gatatacacc ggcatcaacg ggtggcgaat taacaccacc agcgccacca cgccgatggc
    12001 agaaattaac ggcgtcagcc acaacaaacg gccgcgcgac aaataatgac ttaaacgatc
    12061 ggttgcttta ccgctgcgcc ataaccgcca gcacagccag ccaccaaccc acaaaaatac
    12121 cgccgtcgcc agcagcaacc atttaaactc gccgctctgc attccggctg gaatatcgat
    12181 cgccgcgccc gccagaatcc caggcaggaa gtaaaacggt ggccacagca ggcagccgat
    12241 aatattcggc gtaatgaatt ttgccaccgg cagatccagc attcccgcca ccattggcac
    12301 cagcggacgc gtcgggccaa caaagcgacc gaccagaatg gtgaacatgc tgtgttgatg
    12361 caacgcatgt tcagttttat cgagtaatgc tttgtttttc ttcagaaatg accagcgatg
    12421 caacggcttt ttaaaacgcc agcccagcca gaaggagatc cagtcgccca gcaggcaacc
    12481 gacgatccct gccagccagg cgtgccagaa acttaactcg ccgctgccaa tcagcgctcc
    12541 cagccccgcc atcagcaccg taccgggtag aatcaaaccg accagcgcca gcgactccag
    12601 aaaggccacc aacaccaccg ccatcaatga atacacggta gattgggtaa taaagtgttc
    12661 cagcaatgct tgcataatgt gcctgtcaaa tggacgaagc agggattctg caaaccctat
    12721 gctactccgt caagccgtca attgtctgat tcgttaccaa ttatgacaac ttgacggcta
    12781 catcattcac tttttcttca caaccggctc ggaactcgct cgggctggcc ccggtgcatt
    12841 ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg
    12901 cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc
    12961 gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg
    13021 acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc
    13081 tgatgtactg acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa
    13141 tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat
    13201 agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct
    13261 ggtgcgcttc atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc
    13321 attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct
    13381 ccggataacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc
    13441 ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga
    13501 gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct
    13561 caatcggcgt taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat
    13621 cattttgcgc ttcagccata cttttcatac tcccgccatt cagagaagaa accaattgtc
    13681 catattgcat cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaacccaac
    13741 cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa
    13801 cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattggttat ttgcacggcg
    13861 tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct
    13921 ttttatcgca actctctact gtttctccat acccgttttt ttggatggag tgaaacgatg
    13981 gcgattgcaa ttggcctcga ttttggcagt gattctgtgc gagctttggc ggtggactgc
    14041 gccagcggtg aagagatcgc caccagcgta gagtggtatc cccgttggca aaaagggcaa
    14101 ttttgtgatg ccccgaataa ccagttccgt catcatccgc gtgactacat tgagtcaatg
    14161 gaagcggcac tgaaaaccgt gcttgcagag cttagcgtcg aacagcgcgc agctgtggtc
    14221 gggattggcg ttgacagtac cggctcgacg cccgcaccga ttgatgccga cggtaacgtg
    14281 ctggcgctgc gcccggagtt tgccgaaaac ccgaacgcga tgttcgtatt gtggaaagac
    14341 cacactgcgg ttgaagaagc ggaagagatt acccgtttgt gccacgcgcc gggcaacgtt
    14401 gactactccc gctacattgg cggtatttat tccagcgaat ggttctgggc aaaaatcctg
    14461 catgtgactc gccaggacac cgccgtggcg caatctgccg catcgtggat tgagctgtgc
    14521 gactgggtgc cagctctgct ttccggtacc acccgcccgc aggatattcg tcgcggacgt
    14581 tgcagcgccg ggcataagtc gctatggcac gaaagctggg gtggcttgcc gccagccagt
    14641 ttctttgatg agctggaccc gatcctcaat cgccatttac cttccccgct gttcactgac
    14701 acctggactg ccgatattcc ggtgggcacc ttatgcccgg aatgggcgca gcgtctcggc
    14761 ctgcctgaaa gcgtggtgat ttccggcggc gcgtttgact gccatatggg cgcagttggc
    14821 gcaggcgcac agcctaacgc actggtaaaa gttatcggta cttccacctg cgacattctg
    14881 attgccgaca aacagagcgt tggcgagcgg gcagttaaag gtatttgcgg tcaggttgat
    14941 ggcagcgtgg tacctggatt tatcggtctg gaagcaggcc aatcggcgtt tggtgatatc
    15001 tacgcctggt ttggtcgcgt actcggctgg ccgctggaac agcttgccgc ccagcatccg
    15061 gaactgaaag cgcaaatcaa cgccagccag aaacaactgc ttccggcgct gaccgaagca
    15121 tgggccaaaa atccgtctct ggatcacctg ccggtggtgc tcgactggtt taacggtcgt
    15181 cgcacaccga acgctaacca acgcctgaaa ggggtgatta ccgatcttaa cctcgctacc
    15241 gacgctccgc tgctgttcgg cggtttgatt gctgccaccg cctttggcgc acgcgcaatc
    15301 atggagtgct ttaccgatca ggggatcgcc gtcaataacg tgatggcgct gggcggcatc
    15361 gcgcggaaaa accaggtcat tatgcaggcc tgctgcgacg tgctgaatcg cccgctgcaa
    15421 attgttgcct ctgaccagtg ctgtgcgctc ggtgcggcga tttttgctgc cgtcgccgcg
    15481 aaagtgcacg cagacatccc atcagcccag caaaaaatgg ccagtgcggt agagaaaacc
    15541 ctgcaaccgc gcagcgaaca ggcacaacgc tttgaacagc tttatcgccg ctatcagcaa
    15601 tgggcgatga gcgccgaaca acactatctt ccaacttccg ccccggcaca ggctgcccag
    15661 gccgttgcga ctctataagg acacgataat gacgattttt gataattatg aagtgtggtt
    15721 tgtcattggc agccagcatc tgtatggccc ggaaaccctg cgtcaggtca cccaacatgc
    15781 cgagcacgtt gttaatgcgc tgaatacgga agcgaaactg ccctgcaaac tagtgttgaa
    15841 accgctgggc accacgccgg atgaaatcac cgctatttgc cgcgacgcga attacgacga
    15901 tcgttgcgct ggtctggtgg tgtggctgca cactttctcc ccggccaaaa tgtggatcaa
    15961 cggcctgacc atgctcaaca aaccgttgct gcaattccac acccagttta acgcggcgct
    16021 gccgtgggac agtatcgata tggactttat gaacctgaac cagactgcac atggcggtcg
    16081 cgagttcggc ttcattggcg cgcgtatgcg tcagcaacat gccgtggtta ccggtcactg
    16141 gcaggataaa caagcccatg agcgtatcgg ctcctggatg cgccaggcgg tctctaaaca
    16201 ggatacccgt catctgaaag tctgccgttt tggcgataac atgcgtgaag tagcggtcac
    16261 cgatggtgat aaagttgccg cacagatcaa gttcggtttc tccgtcaata cctgggcggt
    16321 tggcgatctg gtgcaggtgg tgaactccat cagcgacggc gatgttaacg cgctggtcga
    16381 tgagtacgaa agctgctaca ccatgacgcc tgccacacaa atccacggcg aaaaacgaca
    16441 gaacgtgctg gaagcggcgc gtattgagct ggggatgaag cgtttcctgg aacaaggtgg
    16501 cttccacgcg ttcaccacca cctttgaaga tttgcacggt ctgaaacagc ttccaggtct
    16561 ggccgtacag cgtctgatgc agcagggtta cggctttgcg ggcgaaggcg actggaaaac
    16621 cgccgccctg cttcgcatca tgaaggtgat gtcaaccggt ctgcagggcg gcacctcctt
    16681 tatggaggac tacacctatc acttcgagaa aggtaatgac ctggtgctcg gctcccatat
    16741 gctggaagtc tgcccgtcga ttgccgtaga agagaaaccg atcctcgacg ttcagcatct
    16801 cggtattggt ggtaaggacg atcctgcccg actgatcttc aatacccaaa ccggtccagc
    16861 gattgtcgcc agcctgattg atctcggcga tcgttaccgt ctgctggtta actgtatcga
    16921 cacggtgaaa acaccgcact ccctgccgaa actgccggtg gcgaatgcgc tgtggaaagc
    16981 gcaaccggat ctgccaactg cttccgaagc gtggatcctt gctggtggcg cgcaccatac
    17041 cgtcttcagc catgcgctga acctcaacga tatgcgccag ttcgccgaga tgcacgacat
    17101 tgaaatcacg gtgattgata acgacacccg cctgccagcg tttaaagacg cactgcgctg
    17161 gaacgaagtg tattacggat ttcgtcgcta agtagccaca tcaggtatgt aacgcctgat
    17221 gcgacgctga cgcgtcttat ctggcctaca cgctgcgatt ttgtaggccg gataagcaaa
    17281 gcgcatccgg cattgttttc cccctcgccc ctttggggag agggccgggg tgaggggaaa
    17341 cgacctctca ggagtcaaca tgttagaaga tctcaaacgc caggtattag aagccaacct
    17401 ggcgctgcca aaacacaacc tggtcacgct cacatggggc aacgtcagcg ccgttgatcg
    17461 cgagcgcggc gtctttgtga tcaaaccttc cggcgtcgat tacagcgtca tgaccgctga
    17521 cgatatggtc gtggttagca tcgaaaccgg tgaagtggtt gaaggtacga aaaagccctc
    17581 ctccgacacg ccaactcacc ggctgctcta tcaggcattc ccctccattg gcggcattgt
    17641 gcatacgcac tcgcgccacg ccaccatctg ggcgcaggcg ggtcagtcga ttccagcaac
    17701 cggcaccacc cacgccgact atttctacgg caccattccc tgcacccgca aaatgaccga
    17761 cgcagaaatc aacggcgaat atgagtggga aaccggtaac gtcatcgtag aaacctttga
    17821 aaaacagggt atcgatgcag cgcaaatgcc cggcgttctg gtccattccc acggcccgtt
    17881 tgcatggggc aaaaatgccg aagatgcggt gcataacgcc atcgtgctgg aagaggtcgc
    17941 ttatatggag atattctgcc gtcagttagc gccgcagtta ccggatatgc agcaaacgct
    18001 gctggataaa cactatctgc gtaagcatgg cgcgaaggca tattacgggc agtaatgact
    18061 gtataaaacc acagccaatc aaacgaaacc aggctatact caagcctggt tttttgatgg
    18121 attttcagcg tggcgcaggc aggttttatc ttaacccgac actggcggga caccccgcaa
    18181 gggacagaag tctccttctg gctggcgacg gacaacgggc cgttgcaggt tacgcttgca
    18241 ccgcaagagt ccgtggcgtt tattcccgcc gatcaggttc cccgcgctca gcatattttg
    18301 cagggtgaac aaggctttcg cctgacaccg ctggcgttaa aggattttca ccgccagccg
    18361 gtgtatggcc tttactgtcg cgcccatcgc caattgatga attacgaaaa gcgcctgcgt
    18421 gaaggtggcg ttaccgtcta cgaggccgat gtgcgcccgc cagaacgcta tctgatggag
    18481 cggtttatca cctcaccggt gtgggtcgag ggtgatatgc acaatggcac tatcgttaat
    18541 gcccgtctga aaccgcatcc cgactatcgt ccgccgctca agtgggtttc tatagatatt
    18601 gaaaccaccc gccacggtga gctgtactgc atcggcctgg aaggctgcgg gcagcgcatc
    18661 gtttatatgc tggggccgga gaatggcgac gcctcctcgc ttgatttcga actggaatac
    18721 gtcgccagcc gcccgcagtt gctggaaaaa ctcaacgcct ggtttgccaa ctacgatcct
    18781 gatgtgatca tcggttggaa cgtggtgcag ttcgatctgc gaatgctgca aaaacatgcc
    18841 gagcgttacc gtcttccgct gcgtcttggg cgcgataata gcgagctgga gtggcgcgag
    18901 cacggcttta aaaacggcgt cttttttgcc caggctaaag gtcggctaat tatcgacggt
    18961 atcgaggcgc tgaaatccgc gttctggaat ttctcttcat tctcgctgga aactgtcgct
    19021 caggagctat taggcgaagg aaaatctatc gataacccgt gggatcgaat ggacgaaatt
    19081 gaccgccgtt tcgccgaaga taaacctgcg ctggcaactt ataacctgaa agattgcgag
    19141 ctggtgacgc agatcttcca caaaactgaa atcatgccat ttttactcga acgagcaacg
    19201 gtgaacggcc tgccggtgga tcggcacggc ggttcggtgg cagcgtttgg tcatctctat
    19261 tttccgcgaa tgcatcgcgc tggttatgtc gcgcctaatc taggcgaagt gccgccgcac
    19321 gccagccctg gcggctacgt gatggattca cggccagggc tttatgattc agtgctggtg
    19381 ctggactata aaagcctgta cccgtcgatc atccgcacct ttctgattga tcccgtcggg
    19441 ctggttgaag gcatggcgca gcctgatcca gagcacagta ccgaaggttt tctcgatgcc
    19501 tggttctcac gggagaaaca ttgcctgccg gagattgtga ctaacatctg gcacgggcgc
    19561 gatgaagcca aacgtcaggg taataaacca ctttcgcagg cgctgaagat cattatgaat
    19621 gccttttatg gcgtgcttgg caccaccgcc tgccgctttt tcgatccgcg cctggcatcg
    19681 tcgatcacca tgcgtggtca tcagatcatg cggcaaacca aagcattgat tgaagcgcag
    19741 ggctacgacg ttatctacgg agataccgac tcgacttttg tctggctaaa aggtgcacat
    19801 tcggaagaag aggcggcgaa aatcggtcgt gcactggtgc agcacgttaa cgcctggtgg
    19861 gcggaaacgc tgcaaaaaca acggctgacc agcgcattag aactggagta tgaaactcat
    19921 ttctgccgtt ttcttatgcc aaccattcgc ggagctgata ccggcagtaa aaagcgctac
    19981 gccgggctga ttcaggaggg cgacaagcag cggatggtgt ttaaagggct ggaaaccgtg
    20041 cgcaccgact ggacgccgct ggcccagcag tttcagcagg agctgtacct gcgtatcttc
    20101 cgcaacgagc catatcagga gtacgtccgc caaaccatcg acaaactgat ggcgggtgaa
    20161 ctggatgcgc ggctggttta ccgtaaacgc cttcgccgtc cgctgagcga atatcagcgt
    20221 aatgttccgc ctcatgtacg cgccgctcgc cttgccgatg aagaaaatca aaagcgcggt
    20281 cgccccttgc aatatcagaa tcgcggcacc attaagtacg tatggaccac caacggcccg
    20341 gagccgctgg actaccaacg ttcaccactg gattacgaac actatctgac ccgccagcta
    20401 caacccgtgg cggagggaat actccctttt attgaggata attttgctac acttatgacc
    20461 gggcaacttg ggctattttg agcaaaaaaa agagttcgcc agataccatt ttgatgcgtg
    20521 acgaatgctt tgccatccag taccatagcg ccctttccat tcctggacct gaataacgcc
    20581 actacctcat aagcacggta gcgggtggtt attgcctgca attaaagata tagagccgaa
    20641 cacatatgcc ttttacactt ggtcaacgct ggatcagcga tacagaaagc gaattgggac
    20701 ttggaaccgt tgtcgcggtg gatgcgcgaa ctgtcacttt acttttccca tctactggtg
    20761 aaaaccgtct gtacgcacgc agtgattccc ccgtgacccg cgtgatgttc aaccctggtg
    20821 ataccattac cagccatgac ggctggcaga tgcaagtcga agaagtaaaa gaagaaaatg
    20881 gcttgctgac ctatatcggt actcgcctgg atactgaaga gtccggcgta gccctgcgtg
    20941 aagttttcct tgatagcaaa ctggtgttca gcaaaccgca ggaccgtctg tttgccgggc
    21001 agattgaccg tatggaccgc tttgcgctgc gttatcgcgc gcgtaagtat tccagcgaac
    21061 agttccgtat gccgtacagc ggcctgcgcg gtcagcgtac cagcctgatc ccgcatcagc
    21121 tcaacatcgc tcatgatgtt ggtcgccgcc acgcgccgcg cgttctgctg gctgacgaag
    21181 tgggtttagg gaaaaccatt gaagccggga tgatcctgca tcagcaactg ctctctggcg
    21241 ctgctgaacg tgtgctgatt atcgtcccgg aaaccttaca gcatcagtgg ctggtagaaa
    21301 tgctgcgccg tttcaacctg cgttttgcgc tgtttgatga tgagcgttat gccgaagctc
    21361 agcacgatgc ttacaacccg ttcgacaccg aacagctggt gatttgctcg ctggattttg
    21421 cccgtcgtag caaacagcgc ctggaacatc tctgtgaagc cgaatgggac ctgctggtgg
    21481 tcgatgaagc gcatcacctg gtgtggagcg aagacgcgcc gagccgcgaa tatcaggcta
    21541 ttgaacaact ggcagagcac gtgccgggcg ttctgctgct gaccgcaacc ccggaacagt
    21601 tgggaatgga aagccacttc gcccgtctgc gtctgctgga cccgaaccgt ttccacgatt
    21661 ttgcgcagtt cgttgaagag cagaaaaatt atcgtccggt tgcggacgcc gttgccatgc
    21721 tgctggcagg taacaaactg agcaatgacg aactgaacat gcttggcgag atgatcggcg
    21781 agcaggatat cgagccgctg ttgcaagcag caaacagcga cagcgaagat gcccagagcg
    21841 cccgtcagga actggtttcg atgctgatgg atcgccacgg caccagccgc gtgctgttcc
    21901 gtaacacccg taacggtgtg aaaggattcc cgaaacgcga gctgcacacc attaagctgc
    21961 cgctaccgac gcagtatcag acggctatta aagtctccgg cattatgggc gcacgtaaaa
    22021 gtgcggaaga tcgtgctcgc gatatgctct acccggagcg tatttatcag gaatttgaag
    22081 gtgataacgc cacctggtgg aacttcgatc cgcgcgttga gtggctgatg ggctacctga
    22141 ccagccatcg ctctcagaaa gtgctggtga tctgcgccaa agctgccact gcgctgcaac
    22201 tggagcaggt actgcgcgaa cgtgaaggta ttcgcgctgc ggtgttccac gaaggtatgt
    22261 cgattatcga acgtgaccgc gctgccgcct ggtttgccga agaagacacc ggcgcacagg
    22321 tactgctgtg ctcagaaatc ggttctgaag gacgtaactt ccagttcgcc agccacatgg
    22381 taatgtttga cctgccattc aacccggatc tgctggagca gcgtattggt cgtctggatc
    22441 gtatcggtca ggcgcacgat attcagatcc atgtgcctta tctggaaaaa actgctcagt
    22501 cggtgctggt gcgctggtat cacgaaggtc tggatgcatt cgagcacacc tgcccgaccg
    22561 gacgcactat ttacgatagc gtctacaacg atctgattaa ctatctggct tcaccggatc
    22621 aaaccgaagg ctttgacgat ctgatcaaaa actgccgcga gcaacatgaa gcgctgaaag
    22681 cacaactgga acaggggcgt gatcgcctgc tggaaatcca ctccaacggt ggcgaaaaag
    22741 cccaggcact ggcagaaagc attgaagagc aggatgacga taccaacctg atcgccttcg
    22801 ccatgaacct gttcgatatt atcggtatca atcaggacga tcgcggcgac aacatgatcg
    22861 tgctgacccc gtccgatcat atgctggtgc cggacttccc tggcctgtcg gaagatggca
    22921 tcaccatcac ctttgatcgt gaagtggcgc tggcgcgtga agatgctcag tttattacct
    22981 gggaacatcc gctgatccgc aacggtctgg atctgattct ttctggcgat accggtagca
    23041 gcacgatttc actgttaaaa aacaaagcgt tgccggtagg tacgctgttg gtggaactga
    23101 tttacgtggt cgaagcccag gcgccgaagc agttgcagct caaccgcttc ctgccaccga
    23161 cgccggtacg tatgctgctg gataaaaacg gcaacaacct ggcggcccag gtggagtttg
    23221 aaacctttaa ccgccagctt aacgcggtta accgtcacac cggcagcaaa ctggttaacg
    23281 ccgtgcagca ggatgttcac gctatccttc agctgggtga agcgcagatc gagaaatctg
    23341 cccgtgcatt gattgatgcc gcacgtaacg aagccgacga aaaactgtct gccgagctgt
    23401 ctcgtctgga agcgctgcgc gcagtgaacc cgaacattcg tgacgacgaa ctgaccgcca
    23461 ttgagagcaa ccgtcagcag gtaatggaaa gcctggatca ggcaggctgg cgtctggatg
    23521 ccctgcgttt gatcgttgta acgcatcagt aacggagccg aaaatgggga tggaaaacta
    23581 caatccaccg caggaaccct ggttggttat cctgtatcag gatgaccaca ttatggtggt
    23641 taacaagccg agcggtttgt tgtctgtgcc gggtcgtctg gaagagcaca aagatagcgt
    23701 gatgacgcgc attcagcgtg attatccgca ggcagaatcg gtgcatcgtc tggatatggc
    23761 taccagcggc gtgattgtag tggcgctgac caaagccgcg gagcgggagt taaaacgcca
    23821 gttccgcgag cgcgagccga aaaagcagta tgtggcccgc gtctgggggc atccatcccc
    23881 cgcagaaggt ctggtggatc tgccgctgat ttgcgactgg ccaaaccgcc cgaaacagaa
    23941 agtctgttac gaaacgggta aacctgcgca gacggaatat gaagtggtgg agtatgtggc
    24001 ggataacacg gcaagagtgg tgttaaaacc gattaccggg cgttcgcatc aattgcgtgt
    24061 gcatatgctg gcgctgggtc atccgattct cggcgatcgt ttttatgcat caccagaagc
    24121 gagagcgatg gcaccacgtt tgttgctaca tgcagagatg ttgacgatta cccatccggc
    24181 gtatggcaat agtatgacgt ttaaagcgcc agcggatttt taatcgtcac gctggcgcag
    24241 ttcggtaggc ggataaggcg ttcacgccgc atccggcaat tgtgcgccga ttgcctgatg
    24301 cgacgcgggc cgcgtcttat caggcctaca gtttaaaact tatttaaacc ctttctgctg
    24361 ctttatcagc tcatatgcct gctgaatttc ctgcgctttc tgcttcgcca tctccatcat
    24421 ctcaggcggc aaacctttcg ccaccagctt atcgggatgg tgttcactca tcagcttacg
    24481 gtaggcacgt ttgatggtgg tcgcatcatc cgtcggcttc acgcccagca cattacaggc
    24541 atcttccagc gtcgggccac gctgcgcttg ctgccagtta ccaccgccag attgctgctg
    24601 ataaccgccg ccaaactgtg caccgccctg catcatgcgc aaaaactggt caaactgggc
    24661 gcgggagatc cctaattctt cagcaatgac atacagcacc gcccgttcat tcgggtgcag
    24721 tgaaccatca gcaaacgccg cctgaatctg gatctccaga aacatacgaa ttaagtcaaa
    24781 acgaccaaag cagacactgc gaaactggcg catcttttcg cgcagcgggt aattatctga
    24841 ttttcccact cggaacgcgt tttgcgccgc agtacgggaa gcgccatgaa ggttcattcg
    24901 gtccatcaac tggctggcga tatgaatatc agcctccgtg acgcgacctt tggatttggt
    24961 taaatgcccc atcacttcaa aagtggtggc aaaaaacagc gcctgacgct cacgctggtt
    25021 ggcgaaccac gccattttac ggctacgggc tttatcaaac atatggccaa ttaacaggcc
    25081 taacactacg ccccaaaagc cgccgcccat cagtaaggcc acggccacgc caatgatttt
    25141 tccccaatac tgcatatatt ccccaaatcg acacacggat atcagggcta tctcccacaa
    25201 tataaaggtg cttttaccgt tttccggctg cggtcaattg tgggacatcg cctataattt
    25261 gcattatcat acctgtcatt caatgccgtg cctaacacca ctgacgctat tcggacagga
    25321 ttaacactag cgtagagatg acgagtacgt tagtctctga ccgtttgtca cgcgcaacgt
    25381 taccgatgat ggaacaataa aatcaacgta tgaaaaaacg tatccccact ctcctggcca
    25441 ccatgattgc caccgccctt tatagtcaac agggactggc agccgacctc gcctcacagt
    25501 gcatgttggg cgtgccaagc tatgaccgtc ctctggtaca gggcgatacc aatgacttac
    25561 ccgtgactat caatgctgac cacgcgaaag gggactaccc ggatgacgcc gtgtttactg
    25621 gcagcgtgga tatcatgcag ggtaacagcc gtctgcaggc cgacgaagtg cagctccatc
    25681 aaaaagaggc accaggacaa ccggagccgg tacgtaccgt tgatgcgctc ggtaatgtcc
    25741 attacgacga taaccaggtg atcctcaaag ggccgaaagg ctgggcgaat ctgaacacca
    25801 aagataccaa cgtctgggaa ggtgattacc agatggtggg tcgccagggt cgcggtaaag
    25861 cggacctgat gaaacaacgt ggcgaaaacc gctataccat tctggataac ggtagcttta
    25921 cctcctgtct gccgggttct gacacctgga gcgtggtagg tagcgaaatt attcatgacc
    25981 gcgaagaaca agttgcggag atctggaacg cccgctttaa ggtgggtccg gtaccgatct
    26041 tttatagccc ctatttgcag ttgccggtgg gtgacaaacg tcgctctggt ttcttgatcc
    26101 cgaacgccaa gtacaccacc accaactact ttgagttcta cctgccatat tactggaaca
    26161 tcgcgccaaa tatggatgcc accatcacgc cgcattatat gcatcgtcgt ggcaacatca
    26221 tgtgggagaa cgaattccgc tacctctccc aggcgggcgc tggcttgatg gaactggact
    26281 atctgccttc agataaagtc tatgaagatg aacacccgaa cgatgacagt tcacgtcgtt
    26341 ggttattcta ctggaaccac tccggggtca tggatcaggt gtggcgtttc aacgtcgact
    26401 acaccaaggt cagcgatcct agctacttca atgatttcga taacaagtac ggttccagta
    26461 ctgacggcta cgcaacgcaa aaattcagcg ttggctatgc ggtgcaaaac ttcaatgcca
    26521 ccgtttcaac caagcagttc caggttttca gcgaacagaa caccagtagc tactcggcag
    26581 agccgcagtt agacgttaat tactaccaga atgatgttgg tccatttgat acgcgtattt
    26641 acggccaggc agtgcacttt gttaacacca gagacgacat gcctgaagca acccgtgttc
    26701 acctggaacc gaccatcaat ttgccgctct ctaataactg gggcagcatc aataccgaag
    26761 cgaagttgct ggcaacccat tatcagcaaa ccaatcttga ctggtataac tccagaaaca
    26821 cgaccaagct ggacgaatcc gttaaccgcg taatgccgca attcaaagtt gacggcaaaa
    26881 tggtctttga acgcgatatg gaaatgctgg ctccgggtta tacccaaacg ctggaaccgc
    26941 gcgcgcagta tttgtacgtg ccgtatcgcg atcagagcga catctataac tacgactcgt
    27001 ctctgctgca atctgactac tctggcctgt tccgggaccg gacttacggc ggtcttgacc
    27061 gtattgcctc cgctaaccag gtgacgaccg gtgtcacatc tcgcatatat gatgatgctg
    27121 ccgttgaacg ttttaatatt tccgttggtc aaatctacta tttcacggag tctcgcactg
    27181 gcgatgacaa cataacatgg gagaatgacg acaaaacggg ttcactggtg tgggcaggcg
    27241 atacttactg gcgtatctcc gagcgttggg gattgcgtgg cgggattcag tacgatacac
    27301 gtctggataa cgtagcgacc agtaactcca gcattgaata ccgtcgggat gaagaccgtc
    27361 tggtacagct gaattaccgt tacgccagcc cggaatatat tcaggctacg ctgcctaagt
    27421 actattccac tgctgagcaa tataagaatg gtatttcgca ggtaggtgct gtcgccagct
    27481 ggccaattgc cgatcgttgg tccattgttg gggcctacta ctacgacacc aatgcgaaca
    27541 agcaagccga ctctatgtta ggtgtgcaat acagctcctg ctgctatgca attcgcgtcg
    27601 gttacgagcg gaagctgaac ggttgggata acgataaaca acatgcggta tatgacaacg
    27661 caatcggctt taacatcgaa cttcgcggcc tgagctccaa ctacggtctg ggtacgcaag
    27721 agatgctgcg ttcgaacatt ctgccgtatc aaaacacttt gtgatttgtt gatttaccac
    27781 gtaatccgca gtgcggttaa ttgaaatgga aaaagtatga agaactggaa aacgctgctt
    27841 ctcggtatcg ccatgatcgc gaataccagt ttcgctgccc cccaggtagt cgataaagtc
    27901 gcagccgtcg tcaataacgg cgtcgtgctg gaaagcgacg ttgatggatt aatgcagtcg
    27961 gtaaaactga acgctgctca ggcaaggcag caacttcctg atgacgcgac gctgcgccac
    28021 caaatcatgg aacgtttgat catggatcaa atcatcctgc agatggggca gaaaatggga
    28081 gtgaaaatct ccgatgagca gctggatcag gcgattgcta acatcgcgaa acagaacaac
    28141 atgacgctgg atcagatgcg cagccgtctg gcttacgatg gactgaacta caacacctat
    28201 cgtaaccaga tccgcaaaga gatgattatc tctgaagtgc gtaacaacga ggtgcgtcgt
    28261 cgcatcacca tcctgccgca ggaagtcgaa tccctggcgc agcaggtggg taaccaaaac
    28321 gacgccagca ctgagctgaa cctgagccac atcctgatcc cgctgccgga aaacccgacc
    28381 tctgatcagg tgaacgaagc ggaaagccag gcgcgcgcca ttgtcgatca ggcgcgtaat
    28441 ggcgctgatt tcggtaagct ggcgattgct cattctgccg accagcaggc gctgaacggc
    28501 ggccagatgg gctggggccg tattcaggag ttgcccggga tcttcgccca ggcattaagc
    28561 accgcgaaga aaggcgacat tgttggcccg attcgttccg gcgttggctt ccatattctg
    28621 aaagttaacg acctgcgcgg cgaaagcaaa aatatctcgg tgaccgaagt tcatgctcgc
    28681 catattctgc tgaaaccgtc gccgatcatg actgacgaac aggcccgtgt gaaactggaa
    28741 cagattgctg ctgatatcaa gagtggtaaa acgacttttg ctgccgcagc gaaagagttc
    28801 tctcaggatc caggctctgc taaccagggc ggtgatctcg gctgggctac accagatatt
    28861 ttcgatccgg ccttccgtga cgccctgact cgcctgaaca aaggtcaaat gagtgcaccg
    28921 gttcactctt cattcggctg gcatttaatc gaactgctgg atacccgtaa tgtcgataaa
    28981 accgacgctg cgcagaaaga tcgtgcatac cgcatgctga tgaaccgtaa gttctcggaa
    29041 gaagcagcaa gctggatgca ggaacaacgt gccagcgcct acgttaaaat cctgagcaac
    29101 taatggttaa aacccaacgt gttgtgatca ctcccggcga gcccgccggg attggcccgg
    29161 acttagttgt ccagcttgca cagcgtgagt ggccggtcga actggttgtt tgtgccgatg
    29221 ccactctcct taccgaccgg gcagcgatgc tcggtttgcc gctcaccctc cgcccttatt
    29281 cccccaactc ccctgcacaa ccgcaaacta cgggcacatt aacgctactt cctgtcgcgc
    29341 tacgtgaatc tgtcactgcg gggcagttag cgattgaaaa tggacattac gtggtggaga
    29401 cgctggcgcg agcgtgtgat ggctgtctga acggtgaatt tgctgcgctg atcacaggcc
    29461 ccgtgcataa aggcgtcatt aacgacgcag gcattccgtt taccggtcat accgagtttt
    29521 tcgaagagcg ttcgcaggcg aaaaaagtgg tgatgatgct ggcgaccgaa gaacttcgcg
    29581 tggcgctggc aacgacgcat ttaccgctgc gcgatatcgc agatgctatc acccctgcac
    29641 ttttgcacga agtgattgct attttgcatc acgatttgcg tacaaaattt ggtattgccg
    29701 aaccgcgcat tctggtctgc gggctgaatc cgcacgcggg cgaaggcggt catatgggta
    29761 cggaagagat agacaccatt attccggtgc tcgacgagct gcgggcgcag gggatgaaac
    29821 tcaacgggcc gctgcctgcc gataccctgt ttcagccgaa atatctcgat aacgccgacg
    29881 ccgtgctggc gatgtaccac gatcagggtc ttcccgtgct aaaataccag ggcttcgggc
    29941 gcggtgtgaa cattacgctg ggcctgccct ttattcgcac atcagtggac cacggcaccg
    30001 cgcttgaact ggcgggacgt ggcgaagccg atgtcggcag ttttattacg gcgcttaatc
    30061 tcgccatcaa aatgattgtt aacacccaat gaataatcga gtccaccagg gccacttagc
    30121 ccgtaaacgc ttcgggcaaa actttctcaa cgatcagttc gtgatcgaca gcattgtttc
    30181 tgccattaac ccgcaaaagg gccaggcaat ggtcgaaatc ggccccggtc tggcggcatt
    30241 gaccgagccg gtcggcgaac gtctggacca gctgacggtc atcgaacttg accgcgatct
    30301 ggcggcacgt ctgcaaacgc atccattctt aggcccgaaa ctgacgattt atcagcagga
    30361 tgcgatgacc tttaactttg gcgaactggc cgagaaaatg ggtcagccgc tgcgtgtttt
    30421 cggcaacctg ccttataaca tctccacgcc gttgatgttc catctgttta gctatactga
    30481 tgccattgcc gacatgcact ttatgttgca aaaagaggtg gtgaatcgtc tggttgcagg
    30541 accgaacagc aaagcgtatg gtcgattaag cgtcatggcg caatactatt gcaacgtgat
    30601 cccggtactg gaagttccgc cgtcagcctt tacaccacca cccaaagtgg attccgccgt
    30661 cgtgcgcctg gttcctcatg caacgatgcc tcacccggtt aaagatgttc gtgtgttgag
    30721 ccgcatcacc accgaagcct ttaaccagcg tcgtaaaacc attcgtaaca gcctcggcaa
    30781 cctgtttagc gtcgaggtgt taacgggaat ggggatcgac ccggcgatgc gagcggaaaa
    30841 tatctctgtc gcgcaatatt gccagatggc gaactatctg gcggagaacg cgcctttgca
    30901 ggagagttaa cgatgatcaa ttcgccccga gtgtgtattc aggttcaaag cgtctacatt
    30961 gaggcacaat cttcacctga taatgaacgt tacgtttttg cttataccgt aaccatacgc
    31021 aatctggggc gagcgccagt gcagttgttg gggcgttact ggctgatcac caatggcaat
    31081 ggccgtgaaa ccgaagtcca gggcgaagga gtggttggcg tccagccact tatcgcgcct
    31141 ggcgaagagt accagtacac cagcggtgca atcattgaaa ccccgctggg caccatgcag
    31201 ggtcactacg aaatgatcga tgaaaatggc gtccctttca gcatcgacat tcccgtattc
    31261 cgactcgccg ttcccacact cattcattaa aagaatatgg cgacatacct tattggcgac
    31321 gttcatggtt gttacgatga actgttcgca ttgctgcata aagtagaatt tacccctggg
    31381 aaagataccc tctggctgac gggcgatctg gtcgcgcgcg ggccgggttc gctggatgtt
    31441 ctgcgctatg tgaaatcctt aggcgacagc gtacgtctgg tgctgggcaa tcacgatctg
    31501 catctgctgg cggtatttgc cgggatcagc cgcaataaac cgaaagatcg cctgacaccg
    31561 ctgctggaag cgccggatgc cgacgagctg cttaactggc tgcgtcgcca gcctttgctg
    31621 caaatcgacg aagagaaaaa gttggtgatg gcccacgcag ggatcacgcc gcagtgggat
    31681 ctacagaccg ccaaagagtg cgcgcgcgat gtagaagcgg tgctatcgag tgactcctat
    31741 cccttctttc tcgacgctat gtacggcgat atgccaaata actggtcacc ggaattgcgg
    31801 gggctgggaa gactgcgttt tatcaccaac gcctttaccc gtatgcgttt ttgctttcca
    31861 aacggtcaac tggatatgta cagcaaagaa tcgccggaag aagcccctgc cccactgaaa
    31921 ccgtggtttg cgattcctgg ccctgtcgct gaagaataca gcatcgcctt tggtcactgg
    31981 gcatcgctgg aaggcaaagg tacgccggaa ggtatttacg cgctggatac cggctgctgc
    32041 tggggtgggt cattaacctg cctgcgctgg gaagataagc agtattttgt ccagccgtcg
    32101 aaccggcata aggatttggg tgagggagag gcggtcgcgt cttaacgaat taccattcag
    32161 ggaggaataa tggctgacta ttttgaaata cgtgctgatt acaatcagca caccatcacg
    32221 atttatcagg cttacaatga tgcaatcgct gacgtcgccg tacgtgacgg gcgatttggc
    32281 gcaccttttt cattcaaccg catgacctgg attaaaccct cttttatgtg gatgatggag
    32341 cgtagcaact ggggactgaa aaaagatcaa caacacattc tcgcaatccg aatcaaacgc
    32401 accttctttg atacattgct ggaacaagcg gtgctaacca cgccggaagc ccatgtgtat
    32461 ccacatgcag gaatttggga aacgttattt gcacaagcaa atgtgtatgt tcagtgggac
    32521 ccagaacgca gcataaatgg taaaaaactg gagcaccgat cattacagct tggtatttct
    32581 cgtaacttga tcagtcaatt taacgaagac gcgattgttg ctattgatga tctgacgccg
    32641 ctggtgcgaa agtgtcataa ccttttgata aacggcaaaa caacacaggc gaaatccttc
    32701 ctgccgccag aaaaaattta cccagttagt gcagcagcga gaaaggcttt gggaatgaaa
    32761 aattcctgag acaaatacgc acctattaag cggaaaatat ctaagtgcga caaatcaaca
    32821 ccgggcttaa taccagagaa attattctgg aaatgattga cgaagcagat gccccccccg
    32881 tttgctgagc agcggacctg gcatcgcagg cgaatcgctt ttctatgtga ttgcatcagc
    32941 gttgctgtcg actacggctt cttctgttct gtctctcagc agtcaataat gaacataacc
    33001 aagatggctg aagatgtatg gataacagaa agtaaaacga cccaactcat tgatatttgg
    33061 caacaactta aacagcttta tccgccagaa actattccct acgcccagaa gttacccact
    33121 aaggaattgc ttgaactgct actttctagt cctgacgatc ttcgaataat tgaaattcct
    33181 gacatcatcg gtgatgtttt tgatgaccaa acctggattc gattattcaa aaaacacttt
    33241 gctgatgtta ttcagctacg atgtgatgtg taacaatcca aaatatatcg ccagacgata
    33301 gaccaaaaag aaagtattta gcagatactc aacattctga cttctgtaca gaaatagcag
    33361 agtttaaaaa cttaaaactt ccggtactca tcagagaaac atccagtttc ttctgcaaat
    33421 tcatttaatg ccgagatggc atcctcgttt acttcattcc actgtagagt ggcctgaagg
    33481 tttagaccta cgcaatgagc gcgactcaca aaatcgcgct cactggtaaa atcaaacgtc
    33541 ttatcgttca atttgtagtc ctggcaaaaa attaccgccg ctccagaatc tcaaagcaat
    33601 agctgtgaga gttctgcgca tcagcatcgt ggaattcgct gaataccgat tcccagtcat
    33661 ccggctcgta atccgggaaa tgggtgtcgc cttccacttc tgcgtcgata tgcgtcagat
    33721 acagtttctg cgcttttggc aggaactgct cataaacgcg accgccgcca atcaccatga
    33781 tttctggtac gtcaccacac gccgcgatgg cttcatccac cgacttcacc cacgttacgc
    33841 gatcgtccgt acccggttga ctgctgagga taatattttt gcgtcctggc aacggacgac
    33901 cgattgattc ccaggtatgg cggcccataa tcacgggttt atttaaggtg ttgcgtttaa
    33961 accaggcgag atcggcaggc aggttccacg gcatggcgtt ttccatgccg ataacgcgat
    34021 ctaccgctaa cgccgcaatc agactgatca ttgagatttc ccgataaaaa aaattgtcgc
    34081 cactatacgt aaagcgtaaa ccgtcgtcga ctggtgcgag gatgatgttg aggaaaattt
    34141 tatattctgc tggcgagtcc acgctctctc cctggactcg ccgcattaca atgaaacaaa
    34201 aacaaacagt tagctgtaaa gtgtgattta cgtcactctt tattaggatg agggtttcgt
    34261 ttccggttca tccgccatgt tgccggtatg tttaccttct tcggttccct gccagccatg
    34321 tcgttgaatt aatgacagat gttcgcggtc ctcggtaatg atctcactta acatcgcgct
    34381 ggtgcgttta tagaccgccg cgcgggcttt ggtgtcgttc tcaaccattg ccatctcttc
    34441 caccatctga atattaaagc ggcggaacac atcggcacgt tctcgcgctt catacggccc
    34501 cagacctaaa ctttccagtg ccagacgccc ggttttcagc gcaccttcga aggtttcacg
    34561 ctccggcttt tcaacgcctg cctgacgcaa acgaatgtag tggtcgacat cgcgggcgcg
    34621 ggcaataatc tgcaaatgcg ggaaatgttc tttcaccatc tccgtcagtt gcaggttggt
    34681 ttgcggatca tcaatagcat taatcagcac ttccgctttc gccgctccgg cagattccag
    34741 taaatccatc cgcgttgcat cgccataaaa cactttcata ccaaatttac gcagcgtttc
    34801 gatatggtcc ggatcgtgat cgagcaccac cattttcacc ccgctggaga gcagtaaacg
    34861 tccggtaatc tgcccaaaac gaccgaatcc ggcgataatc acgcgcggct gttcttcgtc
    34921 gatctcatcg gcttcacgcg cttcctcagt agaagattgc tcaaggcgat tgaggatcac
    34981 cagcagaatc ggcgttgctg ccatcgacag cgccaccgcc agggtcagcg atttcgccca
    35041 ctccggctcc agcacattcg ccatctgcgc cgcgccaaat accacaaagg caaactcact
    35101 gccctgccct aacaacaccg caaaccaacg acgctgttta tttggcactt gcaacggtcg
    35161 ggcaatcagc cacagcatgg cgattttgat gatgaggaaa ccgagcagca aaatgacaat
    35221 gcgcaatggg ttttcaagca gcgtgccaaa gtctatcgac atgccaacac cgatgaaaaa
    35281 cagccccaac agcaaacctt taaatggttc gatatcgctc tccagcgcat gacggtattc
    35341 cgagcttgcc agcagtacgc ccgccagaaa cgcgcccatc gccatcgaca agccgacctc
    35401 ttccagcagc aaaccaaagc caaacacgag gaataacgcc acggcactaa acacttcccg
    35461 caagccagag cgggctacaa aacgcagcgc cggacgcgtg acatagcgcc ccagcaatac
    35521 caccagcacc agcgcacccg ccacttttaa cgccgagaga gcaaatgcgc ccatcgtcgt
    35581 cgaggcactg ctcgttgcca gtagcggaat catcgccacc agcgggatcg ccgcgatatc
    35641 ctggaacagc agcaccgcaa aggcactgcg acccatttgc gtcaccatca gattgcgttc
    35701 attcatcgcc tgcatggcaa tcgccgtaga ggagagcgcc agcgtcatgc cgatcaattc
    35761 cgcgacctgc cagcgcagcc caagtaacat gcagaacagc cccagcaggc cgccgcaaat
    35821 caccatctgc aatgcgccac cgccgaacac tgccgcacgc agcttccaca gcctttgtgg
    35881 atcgagttcg aggccgataa taaacagcat cagcaccacc ccaatctcgg caaagtgcag
    35941 aatagattcg gcatcggtca ccagtcgcag cccccacggg ccaataatgc agccggcgat
    36001 caggtagcca agtaccgatc ccagaccaag acgtaccgca atgggtacaa tcagcgctgc
    36061 cgaaccgaga taaatcagcg cctgaatcag cgtatggcta tccatgatgg gcctcctgcc
    36121 attccagcag acgttgctta tagtgacgcg cctgcccttc gagggtttcg tcgtcacaaa
    36181 taaaggtgca gtgcatggca aacggtggca gccagttcag tccgcagtag attgccgtcg
    36241 cctgtagcgg ctgcgacagc acatcaaagc ccggatgcgc accaatttca aaatggcttt
    36301 ccccgccgcc ggtcgtcacc gcccacagca aatgtttgcc atgcagcgcc gtgccgccat
    36361 gaccgtaagc ccagccgtgg gagaaaactt tatcgatcca aagtttgagg agcggaggaa
    36421 tgctgtacca ctgcatcggg tgctgccaga cgatcagatc ggcgcgagac agcgcctcct
    36481 gctcggcggc aatatcgata ttgaagtcag gatagagttg ataaagagag cgaatttcga
    36541 cgccttccag cgtccttgcc tgttcaagca tccgtttatt cgcatgggaa tgatgcggat
    36601 acggatgcgc ataaattata agaatcatag gtagcccgcg tgattacgcc tttgttttcc
    36661 cacaaagtgt agtcatatat tcacgcggct tatagtcaat aatattgatt cagaaaagag
    36721 agaaatctga acaatttacc ccattctctg cccgcggatc gtcatattgc tggtttgcgc
    36781 cagtgacatc cctcgagtct ccggggcaaa cgctacggaa atcaacaagc caaacagcga
    36841 gatacccgcc cccattagca tcgtgttact gataccgtaa ttattgataa agatcggcag
    36901 tgcccaggtc gaaacaatgg tgccaatacg gcttaaggac ataatcacgc ccacggcaga
    36961 ggcgcggata tctgtcggga agagttcatt aggatagagc cactgcaaat tacccggccc
    37021 gccagagaaa aaggcataca ccgcaaaggc catcactacc agccagatcc ccatatccgg
    37081 gatcagcccc aaaaccgcca gcgccagcgt catcatggca aagctgccaa tcaacaatgg
    37141 acgccgtccg gcagtgttta accacagcat cggcggaata cagccgagca taaagaacag
    37201 gctaatcacc acgttcccca gcgccgcgtt tttgccaaca cccaatccca acaaaccaac
    37261 gatttgcggg ccaaaggtgt aaatggcgaa cattgggatc acctggcagg tccagatggc
    37321 ggcaacaaac agaacaaaag gaaaatggcg gcgattaaac agatcgcgaa aacgggtttg
    37381 ctgcggctgc tcttcatcga aagccaccgg ttcgccaaac agttttatca tcatctcttc
    37441 gcactctttt actcgccctt tgcgtaataa ccagcgggga gattcaggca gttcgaatcg
    37501 accaatcaaa atcaacaaac aggggatcgc cgcgctaccc agcatccagc gccagccgcc
    37561 ttccacatca taaagccagt agccgaccag atcggcgcag gtcgcgccga cataccacat
    37621 ggcggcgata aagctgatgg aaaacgcccg ctgacgggta ctggagaact cggtgatcat
    37681 tgaggtggcg atgggataat ctgcaccgat gacaatgccg ataagtaccc gcatcaccaa
    37741 cagttcgacg ggggatgaaa caaacatcgt cgccaccgat atcacgccga tggcgatgat
    37801 atcaatgagg aacattttgc gccgtccgac tttatcggaa atatagccaa acagcgatgt
    37861 gccaacgaac agcccggcga gcgttcccgc gcccaacaag ccaatccagt cagcgtccag
    37921 ttttagcgcc ggagtcagtt gctccagcgc cacgccaatc attaccagta cataaccatc
    37981 cagaaacgga ccaccgcttc cccacagcaa aatgcggcgg tgaattgagg agaatttgag
    38041 atcgtcaaag tttctggacg gttgcatggg ttattcctgt ttttttgcaa tgagagtcag
    38101 tacggcgtta cgtatcaaac caacatcagc cgtaacggaa ctccacacca aaggtgccgc
    38161 gcgggtattc ccactgttcc agcgccgacc ccagccccag aatgcgacag gtgccgcact
    38221 ccagacatcc ggcgtaatcg aagcgcacac tgccgtcatc ctgcttcttg tacagacctg
    38281 cggggcacgc tttcaccagc agctccaggg cctgtttatc ggcatcggcc ttcacaacaa
    38341 tgtgcggatg ctcttcatcg acattgaatt tattgacgcc cagtttgacg tccacattga
    38401 cgggagaagt cataaaacgg tcactccttt catgccatcc ttgatcagat tgatgaagcc
    38461 cactttcttg ccgtggcgga ggattttctt gcgcatcagt tccggcgcac tgccgtcaat
    38521 ggtgaacagg tcacgcgcca cgcccaccgc cagttccggg tagccgctaa acatgcgtgg
    38581 gttatcaagg aaggccggta gtttctggta catacgcata tcgcgcagcg ggccactctc
    38641 aagatgctga cgatattccg cgagtttttg cttactgaaa tcgtcgcttt tcatcgctga
    38701 aagcacggtt tttgctgcag cttccccggc ggcaatcgcc agatccatac cgcgaatggt
    38761 aaaaccgagg ttcatacaca ttccggcggc atcaccggca atcaatacgc cgtcaccaac
    38821 caactccggc agcatgttga tgcctgcttc cggcactacg tgagcggaat attccaccag
    38881 cttgccgccc gcgatcagcg gtgccactgc cggatgctgt ttgaaatctt ccagcatttg
    38941 cggcaccgat ttttttgcgt catgcagatg atgcagacca caaaccagcc ccagcgacag
    39001 ggtattttca ttggtataaa ggaagccgcc gcccatcagg ccatcggtgg gtgatcccgc
    39061 aaacagacaa gccgcgccct gattaccctg caactgaaaa cggtcttcga ttaccgactt
    39121 cggtaactcg atcagttcct tcacgccaac cgccacatcc gtcggtttaa cgcgttttgc
    39181 catccccagc ttttcggcaa ggatggagtt cactccatca gcaaggatca ccgttttcgc
    39241 ttcaatcaca tcgccatcgg cttctacacc gacgactttg ccatcgcgct gtacgaggtt
    39301 atcgacgcgg atcccggtaa ttaactgcgc gcccgcttct tcggcctgct ccatcagcca
    39361 ggcatcaaat ttactgcgca aaacggagta agaacgctgg gatggcgagg tttcgtcacc
    39421 attgcagtag tccatagtca tcgctgactt ttccgtcata aacgcgagtt tttcatgggt
    39481 gatcaggcgt tctacggggg cggagtcggc gaaaccagga ataatgtgtt ccaggctgtg
    39541 ggcatagaga cgcccgccgg tgacgttctt ggcacctgcg gaattgccac gctcgataac
    39601 taacacttgc gccccttcgc gggcgagcac cagtgcggca accgaaccgg caagcccagc
    39661 accgacgatg atggcgtcaa agatatcttc ggacataaga gctccctgtc agcggcaggg
    39721 ccaaaatgca gccctgccag agtggatcaa cgcgctaaag ctgcggtcag cgccggaagg
    39781 atcttcacgg cgtcaccaac aatgccgtaa tccgcgtact ggaagatcgg cgcattttta
    39841 tctttattga tggcgaaaat ggtttgcgat gcattagcac caaccatgtg ctggatctgc
    39901 ccggagatcc ccaccgccag gtacagttca ggtttcagca tcaggttgga gataccgaca
    39961 tagcgttcgt gctccatcca tttttcgttt tccgccaccg gacgagaaca ggccaactcc
    40021 gcacctatcg ccttgcaaag ctgttctgcc agcgcaatgt tctctttgct gccaataccg
    40081 cgaccgacgc tgaccaccag acgggctttg tcgagatcga cgctgttgct ctggcgcgcc
    40141 tgggttgccg tgcgggtaat cgccacagcc ggagcctgcc actccacggt gtgcgtttcg
    40201 ccagtgcgtg acgcgtctgg ctgagccgca tcgaacgtgc cgctgctgat ggtcagtacc
    40261 gcatacggcg tggcaatgcg ttcttcgcca atcgccagac caccgtaaac catgtgtttc
    40321 actgtcgctt taccgtcctg tacgctgacg gtgctggcat cgttagacac cgccgcttta
    40381 aggcgataac ccagttttgc cgccagtaat ttgccgcgac gggtgtttgg cagcagcacc
    40441 aggccgtctg cgccgtgctg gcgaatagtg tcagccatga caccggcgta atcttcgatc
    40501 atccgatcgt ccggtttgcc gtttaatttc cagacatgat tagctccgag ctggattgcc
    40561 tgtacgccgt cggcatcatt gaggacaaag gtgttgattt gattagctaa agcctgcgca
    40621 ccgttcatca gttccggcag acgagaaggg gtatcgctga atacccagac ttgagaaaac
    40681 gtgttcatag catcccctgt aattaaatga ctttgcgaag attttcagca aatgcggcga
    40741 tctgttcttc gccgtcgcct tcaatcacga tgcgctgacg ttcgcgctgt ttcggcgcag
    40801 caacctgttg ttcagaccag gcgtccacgg cgttaaaacc gatatccgcc gccgaccata
    40861 cctggactgg ctttttcgcc gcgccgagaa tggctttcat cgaaggaatt tgtggggagt
    40921 tgatatcagt ggaaacagca acaaccgcag gcagcggaat gcttaaggtt tcggtttcat
    40981 cttccagttc gcgctcaacg gtgagggtat ctgcagtcag ggagataatt ttgctgacgc
    41041 cgttaactgc cggaatattg aggatttcgc ccaccagcag accaacctgc tgggcataaa
    41101 ggtcggaaga accatcgcca cagaggatca aatcaaagcc tgctttctgg gcggctgcag
    41161 ccagtgcgct cgccgtttgt tgcggcagtg cctgctcgaa ctggtcatca atcaccacaa
    41221 tcagttcatc cgggccgcgc gatagcacat ctttacgccc tttggcgttg gtcagggctt
    41281 taccgcccac acttaaggcc gtcacctgcg cctctgctgc ctgttgcttt agctggcaag
    41341 ccgcttcaat agcgttcaga tcgtattggc ttattttggc atcggctttg ctgaagtcta
    41401 atgaaccatc agcattattg accgcaatat cctgttcatc aggcacgcac ttatagcaag
    41461 taataatctt cattgcatct ccagaaatca tgaaggaaac gtcgttcata tcggtggtgt
    41521 taattacaga tatcacgccc tttaatttgg aaataaaact ttaatcacca atattgaaaa
    41581 tgtcacacgc agataaatcc aactttcaat attgttaagt tcctcaccaa tattgaaaac
    41641 atcgcgtagc aaaaagaaat tttcaatatt gctttatgga tcaccaatat tgaaagtcag
    41701 atgctatttt tttactcaca acacaacata acaaactgat taataatcaa aattaacgag
    41761 aaaagtgccc tctattcacc cgaaacaaaa atgtgataac aatcacagaa tgcagcttat
    41821 tgaataccca ttatgagtta gccattaacg cgtccacgag gttaataata attatattaa
    41881 atgttaacaa aaataaaaca aacgggaacg caaaataaat attcgttttc acagtggaat
    41941 taactcatga agaatgaaaa gagaaaaacg ggaatagaac cgaaggtttt ctttccgccg
    42001 ttaataatcg tcggcatact ttgttggctt acagtcagag atctggatgc agcgaatgtc
    42061 gttattaatg ctgtattcag ttacgtcacc aatgtatggg gatgggcatt tgaatggtat
    42121 atggtggtga tgcttttcgg ttggttctgg ctggtgtttg gcccgtatgc caaaaagcgt
    42181 ttaggtaacg aaccacctga atttagtacc gccagttgga tctttatgat gttcgcctcc
    42241 tgtacgtctg ctgccgtact gttctgggga tcgattgaga tctactacta catctccacc
    42301 ccgccgtttg gcttagaacc gaactcgaca ggggcgaaag agttggggct ggcttacagc
    42361 ttgttccact ggggacctct gccgtgggcc acttacagct tcctttcagt cgccttcgct
    42421 tacttcttct ttgtccgcaa aatggaagtg attcgcccca gctcgacact ggtgccgctg
    42481 gtaggtgaaa aacacgccaa agggttgttc ggcactatcg tcgacaactt ctatctcgtc
    42541 gccttgatct tcgcgatggg taccagtctg ggccttgcca cgccgctggt gaccgagtgt
    42601 atgcaatggt tgtttggcat tccgcatacc ctgcaactgg acgctatcat cattacctgc
    42661 tggattatcc tcaacgccat ttgcgtcgct tgcggtctgc aaaaaggggt acgtatcgcc
    42721 agtgacgtgc gtagttacct gagcttcctg atgctgggtt gggtgttcat tgtcagcggt
    42781 gccagcttca tcatgaacta cttcaccgat tcggtgggga tgttgctgat gtatctgccg
    42841 cgcatgttgt tctataccga tcccatcgct aaaggcggct tcccgcaggg ctggaccgtg
    42901 ttctactggg catggtgggt gatttatgct atccagatga gtatcttcct cgcccgcatc
    42961 tcccgtggtc gtactgtgcg tgaactgtgc ttcggcatgg tgctggggct gacagcgtca
    43021 acctggatcc tgtggactgt actcggtagt aacactctgc tgttgataga taaaaacatc
    43081 atcaacattc caaatctgat cgaacagtac ggtgtggcgc gcgccatcat tgaaacctgg
    43141 gccgctctgc cactcagcac cgccaccatg tggggcttct tcatcctctg ctttattgcc
    43201 accgttacgc tggttaacgc ctgctcttat accctggcga tgtccacttg ccgcgaagta
    43261 cgcgatggtg aagaaccacc tctgctggtg cgtatcggtt ggtcaattct ggttggcatt
    43321 atcggtattg ttctgctggc gctcggcggc ctgaaaccga ttcaaaccgc cattatcgcc
    43381 ggaggatgcc cgctgttctt cgtcaacatt atggtgacgc tctcctttat taaagacgcg
    43441 aaacagaact ggaaagatta attaaccccc aaaatatcaa gaggttgaaa gatggatttt
    43501 aatttaaatg atgagcagga actgtttgtc gccggtatcc gcgaactgat ggccagcgaa
    43561 aactgggagg cctattttgc cgagtgcgac cgtgacagcg tctacccgga acgttttgtc
    43621 aaagcactgg cggatatggg tatcgacagt ctgctgatcc ctgaagagca cggtggtctg
    43681 gacgcggggt ttgttactct cgccgccgtg tggatggagc tgggacgtct gggggcacca
    43741 acctatgtgc tgtaccagtt gccgggcggg ttcaacacct tcctgcgcga aggcacacaa
    43801 gagcagatcg acaagattat ggctttccgc ggcaccggta agcagatgtg gaactcagcg
    43861 attaccgaac cgggcgcggg ctccgacgtg ggtagcctga aaacgactta tacccgtaga
    43921 aatggtaaga tttatcttaa tggtagtaag tgttttatta ccagcagcgc ctacaccccg
    43981 tacatcgtgg tgatggcgcg cgacggggct tctccggaca aacctgtcta caccgaatgg
    44041 tttgttgata tgagcaaacc gggcatcaaa gtgaccaaac ttgaaaagct cggtctgcgt
    44101 atggatagct gctgtgaaat cacctttgac gacgtggaac tggacgagaa agacatgttc
    44161 ggtcgggaag gtaacggctt taaccgcgtc aaagaagagt tcgaccatga acgtttcctg
    44221 gtagccctca ccaactacgg tacggcgatg tgcgcctttg aagatgcggc gcgctacgcc
    44281 aatcagcgcg tgcagtttgg cgaggctatt ggtcgtttcc agttgattca ggaaaaattc
    44341 gcccacatgg cgatcaaatt aaactccatg aaaaacatgc tgtatgaagc agcgtggaaa
    44401 gcagacaacg gcaccatcac ctctggcgat gcagcgatgt gcaaatactt ctgcgccaat
    44461 gcggcatttg aagttgtgga tagcgcaatg caggtgctgg gcggtgtcgg gattgcgggc
    44521 aaccaccgca tcagccgctt ctggcgtgat ctgcgtgtag accgcgtctc cgggggatct
    44581 gacgaaatgc agatcctgac gctgggtcgc gcggtgctga agcaataccg ctaagtccgt
    44641 gcgctgcctg atgcgacgct aatgcgtctt atcaggccta cgggagcacg aatgtaggcc
    44701 ggataaagcg tttacgccgc atccggcagt catgcgctac ataaattttt caggagctaa
    44761 taatggatca tctacccatg ccgaaattcg ggccgttggc cggattgcgc gttgtcttct
    44821 ccggtatcga aatcgccggg ccgttcgccg ggcaaatgtt cgcagaatgg ggtgcggaag
    44881 ttatctggat cgagaacgtc gcctgggccg acaccattcg cgttcaaccg aactacccgc
    44941 agctctcccg ccgcaatttg cacgcgctgt cgttaaatat tttcaaagat gaaggccgcg
    45001 aagcgtttct gaaattaatg gaaaccaccg atatcttcat cgaagccagt aaaggtccgg
    45061 cctttgcccg tcgtggcatt accgatgaag tactgtggca gcacaacccg aaactggtta
    45121 tcgctcacct gtccggtttt ggtcagtacg gcaccgagga gtacaccaat cttccggcct
    45181 ataacaccat cgcccaggcc ttcagtggtt acctgattca gaacggtgat gttgaccagc
    45241 caatgcctgc cttcccgtat accgccgatt acttttctgg cctgaccgcc accacggcgg
    45301 cgctggcagc actgcataaa gtgcgtgaaa ccggtaaagg cgaaagtatc gacatcgcca
    45361 tgtatgaagt gatgctgcgt atgggccagt acttcatgat ggattacttc aacggcggcg
    45421 aaatgtgccc gcgcatgagc aaaggtaaag atccctacta cgccggttgc ggtctgtata
    45481 aatgcgccga cggctacatc gtgatggaac tggtgggcat tacccaaatt gaagagtgct
    45541 ttaaagatat tggcctcgca catctgctcg gtacgccgga aatcccggaa ggcactcagc
    45601 ttatccaccg tatcgaatgc ccttacggcc cactggtaga agagaaactc gatgcctggc
    45661 tggcggcaca taccatcgca gaagttaaag aacgctttgc cgaactgaat atcgcctgtg
    45721 ccaaagtact gaccgtaccg gaactggaaa gcaatccaca gtatgttgcc cgtgaatcaa
    45781 tcactcagtg gcaaacgatg gatggtcgca cctgcaaagg gccgaacatc atgccgaaat
    45841 tcaaaaataa ccccggacaa atctggcgcg gaatgccctc acatggcatg gacacggctg
    45901 ccattttgaa aaatatcggc tacagcgaaa acgacattca ggagttggtc agcaaaggtc
    45961 tggccaaagt tgaggactaa acgcgtggcg tcagtcggct tgcctgacta gcgcccgccc
    46021 aataacaaac gaacaaaaat ggatagaggt gcaatggata tcattggcgg acaacatcta
    46081 cgtcaaatgt gggacgatct ggcggacgtt tacggtcata aaacggcgct gatttgtgaa
    46141 tccagcggcg gagtcgttaa ccggtatagt tatcttgagt taaatcagga gattaaccgc
    46201 acggcaaacc tgttttatac gctggggatt cgcaaaggcg acaaggttgc actacatctc
    46261 gacaactgcc cggaatttat cttttgctgg ttcgggctgg caaaaattgg cgcgattatg
    46321 gtgccgatta acgcccgcct gttacgcgag gaaagcgcgt ggatcctgca aaatagccag
    46381 gcgtgcctgc tggtgaccag tgcgcaattc tatcctatgt atcaacagat tcagcaggaa
    46441 gatgccactc aattgcggca catttgcctg acagatgtgg cacttcccgc tgatgatggc
    46501 gtgagttcgt ttactcaact gaaaaatcaa caacctgcca ccttgtgcta tgcaccgccg
    46561 ctattgactg acgatacggc ggaaattctc ttcacctccg gcaccacctc ccgaccgaaa
    46621 ggtgtggtga ttacccatta caacctgcgc ttcgctggat attactccgc ctggcagtgt
    46681 gcactgcgtg acgatgacgt ctacctgacg gtaatgcctg cgtttcatat cgattgccag
    46741 tgtactgcgg cgatggcggc gttttctgcc ggggccacct ttgtgctggt cgagaaatac
    46801 agcgcccgcg ccttctgggg acaggtgcag aagtaccgcg ccaccattac cgaatgtatt
    46861 ccgatgatga tccgtacgtt gatggtgcag ccgccttcag cgaacgatcg gcaacaccgc
    46921 ctgcgggaag tgatgtttta tctcaacttg tcggagcagg aaaaagatgc gttttgtgaa
    46981 cgcttcggcg ttcgcttgct gacgtcttat gggatgacgg aaaccattgt gggcattatc
    47041 ggcgatcgcc ctggcgataa acgacgctgg ccgtcgattg gtcgggcggg gttttgctac
    47101 gaagcggaga tccgcgacga tcacaatcgc ccgctcccgg caggtgagat cggtgaaatc
    47161 tgtattaaag gcgtaccagg gaaaaccatc ttcaaagagt attttctcaa cccgaaagcc
    47221 actgcgaaag tgctggaagc cgacggctgg ctacataccg gcgataccgg atactgcgac
    47281 gaagagggct ttttttattt cgtcgatcgc cgctgcaata tgatcaaacg cggtggcgag
    47341 aatgtctcct gcgtggagct ggaaaatatc atcgccaccc atccaaaaat tcaggatatc
    47401 gtggttgtgg gtattaaaga ttcgattcgc gatgaagcca tcaaagcatt tgtggtgctg
    47461 aatgaaggtg aaacattgag cgaagaggaa tttttccgct tctgcgaaca aaatatggcg
    47521 aaatttaaag tgccctctta tctggagatc agaaaagatc tgccacgtaa ttgctcgggg
    47581 aaaataatta gaaagaatct gaaataaatg aaacagcagg gaactaccct gccagctaat
    47641 aatcacgcta taaaacaata tgcatttttt gcagggatgt tgtcgtccct gaaaaagcaa
    47701 aaatggagaa aaggaatgag tgaatcatta catctgaccc gcaatggatc aattctggaa
    47761 attacccttg atcgtccaaa agcgaatgct attgatgcaa aaaccagctt tgaaatgggc
    47821 gaagtatttc taaatttccg tgacgatccg caattacgtg tcgccattat taccggtgcc
    47881 ggagagaagt tcttttccgc gggctgggat ttaaaagcgg cagcagaagg cgaagcaccg
    47941 gatgctgact ttggcccggg tggatttgcg ggattaaccg aaattttcaa tctcgacaaa
    48001 ccggttatcg cagctgtgaa cggctatgcc tttggcggcg gtttcgaact ggcgctggcg
    48061 gcagatttta ttgtttgtgc cgataacgcc agcttcgccc tgccggaagc caagctgggc
    48121 atcgttcctg acagcggcgg cgtgctgcgt ctaccgaaga tcctgccgcc tgccatcgtc
    48181 aatgaaatgg tgatgaccgg cagacgaatg ggcgcagaag aggcgctgcg ttgggggata
    48241 gtcaaccgcg tggttagcca ggcggaactg atggataacg cccgcgaact ggcgcagcag
    48301 ctggttaaca gcgccccgct ggcgattgcg gcgctgaaag agatcttccg caccaccagc
    48361 gaaatgccgg tagaagaagc gtatcgctat attcgcagcg gcgtgttgaa acactatccg
    48421 tcggttctgc attcggagga tgccattgaa gggccgctgg cgtttgccga gaagcgcgat
    48481 ccggtgtgga aaggacgtta acaaccgtga gctattacgc ctttgagggg ttaattccgg
    48541 tggttcaccc gacggcgttt gtccatccca gtgccgtctt gattggcgat gtgattgtgg
    48601 gagccggtgt ctacatcggc ccactcgcct cactgcgtgg tgactacggg cggttgatcg
    48661 tgcaagcggg agccaatatt caggatggct gcattatgca tggctactgc gacactgaca
    48721 ccatcgttgg ggaaaacggc catatcgggc acggagcgat cctgcatggt tgtgtgattg
    48781 gtcgcgatgc attggtcggg atgaacagcg tgattatgga tggcgcggtc attggcgaag
    48841 agagcattgt tgccgccatg agctttgtca aagcaggctt tcacggcgag aaacgccagt
    48901 tgttgatggg tacgccagcc cgcgctgtac gcagtgttag tgacgacgag ttacactgga
    48961 aacggttgaa taccaaagag tatcaggatc ttgtcgggcg ctgtcatgca tcgttacatg
    49021 aaacacagcc actgaggcaa atggaggaaa atcgcccccg tttgcagggg acgacggatg
    49081 tgacgcctaa acggtgatct tgccggatgc gacctggttg ctggataaga tgctacaaca
    49141 tcgcatctgg caagcaggac gcccggtatc aacgagataa aattaacgac gcatactctt
    49201 tgacaacatc atctgccact tctgctcgcg gttaagttcc gtgagcggaa cggcaggaat
    49261 acgcggcgtc tttcttacgc cgactaattt atcccggctg ttattgcgca ggcgcgcgta
    49321 aatttgctca tcgatatcga ccacttttac cagtcgctgg cactggcatc cccgtccttc
    49381 cagcttatta gggatcatct taacttcgca gcttatttct gcgacttccg acagaatata
    49441 agtcagggta ttaaccgcct tgctgtgttc aatatcgaaa tgaatagaga tctctcttgc
    49501 tatcacccac cgattttcag ccatcatcca ttcggcgatt aacaagtaga gtggtttttc
    49561 aacatatcct tcacacatac gaactccagt ttcacaaatt aaaagtctgg ctatgactcg
    49621 ttttgaagca attaaacaag gccatattaa aattgtggat atatcgatag tatgcaactt
    49681 caccgtcgat aagtgtgagc taaatcctgc ttatgtcatc aaaaatatcg attccccaaa
    49741 agatttactt aatgggcaaa aaaaacggtc ctcatcagag aaccgtatta cttattcgat
    49801 caaattagcg gatgaaaaat atccgccatg acacgctatt atttgatctg cgcgtgcatt
    49861 tcctgcaccg aaattacttt ttcggtcgcg tcggcattca gcgccatcgc ggtagcgaaa
    49921 ccaccgttca gggtggtgtc ataatgcact ttatattgca gcgcactgcg acggatcacg
    49981 cgggagtctt caatcgcgcg acggcctgaa gtggtgttga tgatgtaggt atattcgcca
    50041 ttcttgatgc ggtcctgaat gtgcggacgg ccttcatgca ccttgttcac cagacgcgga
    50101 ttaatgcccg cttcgcccag cacaatcgcc gtgccgtggg tcgcatccag ctcgaagccc
    50161 tgtttcagca gttttgccgc caggtccacc acgcgttctt tatcgccttc gcgcacggaa
    50221 agcaacgcac gaccgtgttt cttcatggtg gagttgctgc ccagctgcgc tttggcaaac
    50281 gcttcagcga aggtgcggcc cacgcccatc acttccccgg tggagcgcat ttctggccct
    50341 aacagcgggt caacacccgg gaatttattg aacggcagca ccacttcttt caccgagtag
    50401 tacggcggga taacttcttt ggttacgccc tgctcagcca gcgatttgcc agccatcaca
    50461 cgcgccgcca cttttgccag cggtacgccg gtggctttgg agacgaacgg aacggtacgc
    50521 gccgcacgcg ggttaacttc aatcaggtag acttcgttgt ttttcaccgc aaactgcacg
    50581 ttcatcagac cgcgcacctg caattcgaag gccagtttct gcacctgctg gcgcatcaca
    50641 tcctgaattt cctgacttaa ggtgtaggct ggcagagaac atgcggagtc accggagtgc
    50701 acgcccgcct gctcgatgtg ctccatgatg ccgccaatca gcaccatttc gccgtcgcag
    50761 atggcatcca cgtcaacttc taccgcgtca tcaaggaagt gatccagcaa cactggcgca
    50821 tcgttagaca cgctgaccgc cgtctggaag tagcgacgca ggtcagcttc gtcatagacg
    50881 atttccatcg cccgaccgcc gagaacgtaa gacggacgta ccaccagcgg gtagccaatc
    50941 tctttcgcct tctctaccgc catttcaata gcggtaacgg tggcgttcgc cggttgtttc
    51001 agtttcagac gctcaaccgc atgctggaag cgttcacggt cttctgcacg gtcgatagca
    51061 tccgggctgg tgccgataac cggtacgcca gcagcttcca gcgcgcgcgc cagtttcagc
    51121 ggggtctgac cgccgtactg aacgataacg cctttcggct tctcaatacg cacgatttct
    51181 agcacatctt ccagtgttac cggctcgaag tagaggcgat cggaagtgtc gtagtcggtg
    51241 gagacggttt ccgggttaca gttaaccata atggtttcgt aaccgtcttc gcgcagtgcc
    51301 agcgaggcgt gtacacagca gtagtcgaat tcgataccct gaccgatacg gttcgggccg
    51361 ccgccgagaa ccatgatttt ttcacggtcg gtagacggat tcgcttcgca ctcttcttca
    51421 taagtggagt acatgtaagc ggtgtcggtg gcgaactctg ccgcacaggt atccacgcgc
    51481 ttataaaccg ggtgcaggtc atactggtcg cgcagcttgc ggatttccgc ttcgcgcacg
    51541 cccgccagtt ttgccagacg cgcatcggca aagcctttgc gtttcagctg gcgcaggaat
    51601 tcagcgttca ggccagtgat gcccacttcc gctactttct cttccagacg caccagttct
    51661 tcaatctgta ccaggaacca gcggtcaata ttggtcaggt tgaacacgcc gtccacagac
    51721 aggcccgcac ggaaggcatc ggcgatgtac cagatacgct ctgcgccagc atctttcagt
    51781 tcgcgacgga ttttggttaa cgcttccggg tcatcgaggc tcactttcgg gtcgaatcca
    51841 gtcgcaccga cttccaggcc gcgcagcgct ttttgcaggg attcctgctg cgtgcgacca
    51901 atcgccatca cttcgccaac cgatttcatc tgagtggtca gacggtcgtt agcaccagcg
    51961 aatttttcga agttgaagcg aggaatttta gtaaccacat agtcgatgga cggctcgaag
    52021 gaggccggag tacgtccgcc agtgatgtcg ttcatcagtt cgtcgagggt gtaacccacc
    52081 gccagtttcg ccgccacttt agcaatcggg aaaccggtcg ctttcgacgc cagcgccgaa
    52141 gaacgggaca cgcgcgggtt catttcgata acaatcaggc gaccgttttt cgggttcacc
    52201 gcaaactgga cattggaacc accggtttca acgccgattt cacgcagcac cgccatcgag
    52261 gcgttacgca tgatttgata ttctttgtcg gtcagcgttt gggctggcgc gacagtgatg
    52321 gagtcaccgg tatggatgcc catcgcatca aagttttcga tagagcagac gatgatgcag
    52381 ttgtcgtttt tatcacgcac cacttccatc tcgtactctt tccagccaat cagcgactca
    52441 tcaatcagca actctttggt cggagagaga tccagaccgc gggcgcaaat ttcttcaaac
    52501 tcttcgcggt tataagcgat accgccgccg ctaccgccca tggtaaagga tgggcgaata
    52561 atgcacggga agcccacgtc agcggcaacc gccagcgctt cttccatcgt gtgtgcgata
    52621 ccggaacgcg cggtttccag accaattttc ttcatcgcta cgtcgaaacg acggcggtct
    52681 tctgctttat caatcgcatc ggcggtcgca ccaatcatag tcacgccgaa ctcttccaac
    52741 acgccctgac gctccagctc cagcgcgcag ttcagcgccg tctgaccgcc catcgttggc
    52801 agcaccgcgt ccgggcgctc tttttcaata atcttgcgta ccacttccca gtgaatcggc
    52861 tcgatgtagg tcgcatcggc catttccggg tcggtcatga tggtcgccgg gttggagttc
    52921 accagaatga cccggtaacc ctcttcgcgc agggctttac atgcttgcgc gccagagtag
    52981 tcaaactcac acgcctgacc gataacaatc gggcccgcac ccagaatcag gatacttttt
    53041 atatctgtac gttttggcat ggctctttta ctcctgatta cttagcggtt ttacggtact
    53101 gctcaattaa ctcgataaag tggtcgaaca acggcgcggc gtcgtgtgga ccagggctgg
    53161 cttcagggtg cccctggaag ctgaacgccg gtttatcggt gcgatgaatg ccctgtaacg
    53221 taccgtcgaa cagggattta tgcgtgacac gcaggtttgc tggtaatgtt gcttcgtcca
    53281 ccgcaaaacc gtggttctgg gcggtgatca tcaccacgtt tttctcaaca tctttaaccg
    53341 gatggttgcc gccgtggtga ccaaatttca ttttgacagt cttcgcaccg ctcgccagcg
    53401 ccagcagctg atgaccgagg cagatgccga agaccggaat atcggtttcg aggaatttct
    53461 ggatggcggt aatggcgtaa tcgcacgggg ccgggtcgcc aggaccgttg gagaggaaga
    53521 taccgtctgg attcattttc agcacatctt ccgcagaagt ttgcgccgga acgatggtca
    53581 ggcgacagcc tctgtccacc aacatgcgca ggatgttgcg cttggcacca aaatcataag
    53641 ccacgacgtg gaacggcagc tcgtcttctt ttttcgcttc tggcaggccg ccggtcaacg
    53701 tccagctccc ttgtgtccag ctataggctt ctgcggtggt cacttctttt gccagatcca
    53761 tgccattcag acctgggaac gcgcgggctt tttctaacgc caacgccgca tccgggttat
    53821 cgcctgcgat aatgcagcca ttctgtgcgc ctttctcgcg cagtaaacgc gtcagcttac
    53881 gggtatcgat atcggcaatc gccacgatgt tatggcgctt caggtaagaa gagaggtctt
    53941 cggtattacg gaagttgctg gcaatcagcg gcaggtcgcg aatcaccaga ccttgtgcat
    54001 gtacctgaga agattcttca tcggcgtcat tagtgccgac attgccaata tggggataag
    54061 taagagtaac gatttgacga gaataggaag gatcagtgag gatttcttga taaccggtca
    54121 ttgaagtatt gaaaacgact tccccaaccg ccgaacctgt tgcccctatg gcccgaccgt
    54181 gaaactgggt tccgtcttcc agaaccaata gcgctgactt aatcaaaaca ccctccagag
    54241 aatattcact cactttattt gcatattaat tcacaatgat gacgtaaatc aatgcaaatc
    54301 tgcttaccga cgaatttctg gcaaacggcg gcattctgga gatatgggcg ctaaaagtca
    54361 acttaatggc gggatttttt atattatttt gtggctcatg catgttttac aggcattaag
    54421 cggcaaaaag atcatataac ctgttctgga aaacgcttgc gcagcaagaa gattgcatga
    54481 aataaaagcg agtagggaaa agtggaccaa atggtcaaaa tttacattaa aacaacaaaa
    54541 accgaagaca aaacatgtaa tttttggcac ttatagacaa tttaaaaaca taacactaaa
    54601 aata
//
LOCUS       DAZGJW010000015        52930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZGJW010000015 DAZGJW010000000
VERSION     DAZGJW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.015"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..350)
                     /locus_tag="ACHKIK_000942"
     CDS             complement(<1..350)
                     /locus_tag="ACHKIK_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO9082159.1"
                     /translation="MNSNYMPFTQRDSLGRYYTKESISALLVSQMKAEKVNNIIDLAS
                     GEGSLTYAALDRWKNAEAYSLDIESRMSKKVCDNLTHIVTDALVHSFPEMLARHQGNF
                     DVAVCNPPFTLPEW"
     gene            complement(592..1800)
                     /locus_tag="ACHKIK_000943"
     CDS             complement(592..1800)
                     /locus_tag="ACHKIK_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9082160.1"
                     /translation="MKLNARQVDAAKPREKAYKLADGAGLYLEVVPSGSRYWRMKYRF
                     NGKEKRMAFGVYPAVSLAQARALRDDAKKKLAEGIDPSLAKKEEKLVRDVQLNNTFQA
                     VALEWHGTKVSRWSEGYASDIIEAFNKDIFPYIGQQPVNEIKPLVLLNVLRRIESRGA
                     TEKAKKVRQRCSEVFRYAIVTGRAEYNPAADLTSAMSGHESKHYPFLTVEELPDFFKA
                     LAGYTGSPLVVLAARLLILTGVRTGELRGAFWSEFDLEKAVWEIPAERMKMKRPHLVP
                     LSTQALEIVQQLKVMSGQYPLVFPGRNDPRKTMSEASINQVFKRIGYTGRVTGHGFRH
                     TMSTILHEEGFNTAWIETQLAHVDKNAIRGTYNHALYLEGRREMMQWYADYINANHNS
                     CISLLVNATK"
     gene            complement(1964..2039)
                     /locus_tag="ACHKIK_000944"
     tRNA            complement(1964..2039)
                     /locus_tag="ACHKIK_000944"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2004..2006),aa:Thr,seq:cgt)
     gene            complement(2154..3407)
                     /gene="proA"
                     /locus_tag="ACHKIK_000945"
     CDS             complement(2154..3407)
                     /gene="proA"
                     /locus_tag="ACHKIK_000945"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO9082161.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(3419..4522)
                     /gene="proB"
                     /locus_tag="ACHKIK_000946"
     CDS             complement(3419..4522)
                     /gene="proB"
                     /locus_tag="ACHKIK_000946"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO9082162.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            4810..5865
                     /gene="phoE"
                     /locus_tag="ACHKIK_000947"
     CDS             4810..5865
                     /gene="phoE"
                     /locus_tag="ACHKIK_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO9082163.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNFYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(5904..6305)
                     /gene="crl"
                     /locus_tag="ACHKIK_000948"
     CDS             complement(5904..6305)
                     /gene="crl"
                     /locus_tag="ACHKIK_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO9082164.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6363..7607)
                     /gene="frsA"
                     /locus_tag="ACHKIK_000949"
     CDS             complement(6363..7607)
                     /gene="frsA"
                     /locus_tag="ACHKIK_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO9082165.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(7699..8157)
                     /gene="gpt"
                     /locus_tag="ACHKIK_000950"
     CDS             complement(7699..8157)
                     /gene="gpt"
                     /locus_tag="ACHKIK_000950"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO9082166.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            8418..9875
                     /gene="pepD"
                     /locus_tag="ACHKIK_000951"
     CDS             8418..9875
                     /gene="pepD"
                     /locus_tag="ACHKIK_000951"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO9082167.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(9932..10546)
                     /gene="prfH"
                     /locus_tag="ACHKIK_000952"
     CDS             complement(9932..10546)
                     /gene="prfH"
                     /locus_tag="ACHKIK_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO9082168.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSLRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(10543..11682)
                     /locus_tag="ACHKIK_000953"
     CDS             complement(10543..11682)
                     /locus_tag="ACHKIK_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO9082169.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(11928..12380)
                     /gene="yafP"
                     /locus_tag="ACHKIK_000954"
     CDS             complement(11928..12380)
                     /gene="yafP"
                     /locus_tag="ACHKIK_000954"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9082170.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(12377..13432)
                     /gene="dinB"
                     /locus_tag="ACHKIK_000955"
     CDS             complement(12377..13432)
                     /gene="dinB"
                     /locus_tag="ACHKIK_000955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO9082171.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(13503..14426)
                     /gene="lafU"
                     /locus_tag="ACHKIK_000956"
     CDS             complement(13503..14426)
                     /gene="lafU"
                     /locus_tag="ACHKIK_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO9082172.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            complement(14429..15292)
                     /gene="motA"
                     /locus_tag="ACHKIK_000957"
     CDS             complement(14429..15292)
                     /gene="motA"
                     /locus_tag="ACHKIK_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO9082173.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(15305..16021)
                     /locus_tag="ACHKIK_000958"
     CDS             complement(15305..16021)
                     /locus_tag="ACHKIK_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HFO9082174.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLVGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(16041..16508)
                     /locus_tag="ACHKIK_000959"
     CDS             complement(16041..16508)
                     /locus_tag="ACHKIK_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HFO9082175.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            complement(16516..17577)
                     /locus_tag="ACHKIK_000960"
     CDS             complement(16516..17577)
                     /locus_tag="ACHKIK_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO9082176.1"
                     /translation="MNPTLLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRQTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAITPTQ
                     PRVSTQPARPKSELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELLSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(17574..17885)
                     /locus_tag="ACHKIK_000961"
     CDS             complement(17574..17885)
                     /locus_tag="ACHKIK_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFO9082177.1"
                     /translation="MEKQRRKLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLDAVKARLYDAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(17890..18282)
                     /gene="fliS"
                     /locus_tag="ACHKIK_000962"
     CDS             complement(17890..18282)
                     /gene="fliS"
                     /locus_tag="ACHKIK_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO9082178.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(18305..19621)
                     /gene="fliD"
                     /locus_tag="ACHKIK_000963"
     CDS             complement(18305..19621)
                     /gene="fliD"
                     /locus_tag="ACHKIK_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO9082179.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSASVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ATGPEITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(19828..20742)
                     /gene="lafA"
                     /locus_tag="ACHKIK_000964"
     CDS             complement(19828..20742)
                     /gene="lafA"
                     /locus_tag="ACHKIK_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HFO9082180.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            21228..22070
                     /locus_tag="ACHKIK_000965"
     CDS             21228..22070
                     /locus_tag="ACHKIK_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFO9082181.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLEKLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSLPDSSPAKKLIITDQIQETVPEEISSTTPPVPVRKKHKVIMGLALAAAVIFIGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQTLAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     LQKC"
     gene            complement(22125..23102)
                     /locus_tag="ACHKIK_000966"
     CDS             complement(22125..23102)
                     /locus_tag="ACHKIK_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HFO9082182.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVAPTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQNTALMQML
                     DKRTALSGGAVDRQLQPVLQGEARVTFHSPDLANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAAQYQTRLTNALRRVGVQLHQQADGISFSTTEKQWPHIERTLSVRTDGDK
                     SAFMPLKTFAEPSEADRLAQSLQQGGAGISQTLESINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(23119..24048)
                     /gene="flgL"
                     /locus_tag="ACHKIK_000967"
     CDS             complement(23119..24048)
                     /gene="flgL"
                     /locus_tag="ACHKIK_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO9082183.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGDLAHVVEQMATGKQILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIESLTESMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEVTLKTYSKVSELNLFSVL"
     gene            complement(24063..25439)
                     /gene="flgK"
                     /locus_tag="ACHKIK_000968"
     CDS             complement(24063..25439)
                     /gene="flgK"
                     /locus_tag="ACHKIK_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO9082184.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGDLSQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTSTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PNITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            complement(25621..25920)
                     /locus_tag="ACHKIK_000969"
     CDS             complement(25621..25920)
                     /locus_tag="ACHKIK_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HFO9082185.1"
                     /translation="MKVNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPKHK"
     gene            complement(25920..27020)
                     /locus_tag="ACHKIK_000970"
     CDS             complement(25920..27020)
                     /locus_tag="ACHKIK_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO9082186.1"
                     /translation="MQNWIKAAVIAVSLALPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVTVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(27035..27700)
                     /gene="flgH"
                     /locus_tag="ACHKIK_000971"
     CDS             complement(27035..27700)
                     /gene="flgH"
                     /locus_tag="ACHKIK_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO9082187.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPKTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FSHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADACISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(27783..28568)
                     /gene="flgG"
                     /locus_tag="ACHKIK_000972"
     CDS             complement(27783..28568)
                     /gene="flgG"
                     /locus_tag="ACHKIK_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO9082188.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(28647..29384)
                     /locus_tag="ACHKIK_000973"
     CDS             complement(28647..29384)
                     /locus_tag="ACHKIK_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HFO9082189.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGAGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLEKNAEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(29384..30586)
                     /gene="flgE"
                     /locus_tag="ACHKIK_000974"
     CDS             complement(29384..30586)
                     /gene="flgE"
                     /locus_tag="ACHKIK_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO9082190.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVMCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFNASD
                     EAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPVDLTVDYSTCTQYGSEFSVTTN
                     AANGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(30610..31314)
                     /locus_tag="ACHKIK_000975"
     CDS             complement(30610..31314)
                     /locus_tag="ACHKIK_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HFO9082191.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGDDIM
                     VQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQVDFS
                     VNPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGEVPF
                     TMISQFGVPDADSDVA"
     gene            complement(31314..31745)
                     /gene="flgC"
                     /locus_tag="ACHKIK_000976"
     CDS             complement(31314..31745)
                     /gene="flgC"
                     /locus_tag="ACHKIK_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO9082192.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(31748..32083)
                     /locus_tag="ACHKIK_000977"
     CDS             complement(31748..32083)
                     /locus_tag="ACHKIK_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HFO9082193.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            32165..32902
                     /gene="flgA"
                     /locus_tag="ACHKIK_000978"
     CDS             32165..32902
                     /gene="flgA"
                     /locus_tag="ACHKIK_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO9082194.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRQWHDYRYTFKIYIPSQIATAAPCTKTPGVTLTSPAEIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMTKSLVARDMPLTANDVELKPYNVSAQRRDVLMELDDAI
                     GLSSKHALQPGRPITKEELVSPVLVGRDQPVMIVYQSAGITASMPGVALKNGRKGEMV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            32983..33261
                     /gene="flgM"
                     /locus_tag="ACHKIK_000979"
     CDS             32983..33261
                     /gene="flgM"
                     /locus_tag="ACHKIK_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO9082195.1"
                     /translation="MKITPTMPGNRPTATTAGSSQPRKTTVTTTTTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSNKVAQVQAMLASGSPQVDTEQLASDMLSFFQN"
     gene            33274..33702
                     /gene="flgN"
                     /locus_tag="ACHKIK_000980"
     CDS             33274..33702
                     /gene="flgN"
                     /locus_tag="ACHKIK_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HFO9082196.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRASEEL
                     LAVNDEITHHYEQLHGHSHQRHSLLKMLGVSVNRDGLAQVFTWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQSFLGSEADFLYQE"
     gene            33829..34758
                     /locus_tag="ACHKIK_000981"
     CDS             33829..34758
                     /locus_tag="ACHKIK_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HFO9082197.1"
                     /translation="MSFIECYEPEYVRKFLARHPGSPLYVRKVWKNEIRLSLSLTDIA
                     SCEAVLNDVRAFDLQLTYRPVEDNSAELSARDAIVNQVILSTLTLRDLTPELSLYAVG
                     ILLSCASKMPGRDGDILARLTTLPQALADHAKKGTLQAQYAQLPPVPQLARQLMTLLG
                     GCAFDWSILPESPRKASLPLQVTLLTLHDANSEALLQQQLQTQWQTTWQQHFATAPWM
                     MRNWLIYRVYHDMIGQTDGADYFPLVCDFYLLRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRASENALLVRQQIQSLCAADPLLSAFSLLT"
     gene            34937..37381
                     /locus_tag="ACHKIK_000982"
     CDS             34937..37381
                     /locus_tag="ACHKIK_000982"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO9082198.1"
                     /translation="MPTPTIIKTLATHREKPFVSVVTPTWNRGAFLPYLLYMYRYQDY
                     PADRRELIILDDSPQSHQHIIDRLTNGTPEAFNIRYIHHPEKLPLGKKRNMLNELARG
                     EYILCMDDDDYYPADKISYTIAMMQKHRALISGSDQIPIWYSHINRIFQSRSFGPHNI
                     LNGTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDK
                     DFIIGASTPVNTALTDIVRDPLLRNAYLSLHNATHHQAINHQAIDQVVVVNLDKRPDR
                     LQQIREELALLHIPPEKITRLAASEDENGQRGRRQSHLQALRLAQQHGWQNYLLLEDD
                     AVILKQEKHIQVLNTLLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAY
                     LVNSRYYPQLAQQMSNDEHSLEDCWQPLLRADKWLACYPSLCYQRPGFSDIQKKTTDN
                     IARYFNKLPVTTKPSALPIADTIGFFMETSFHYALYRPIITALQAQGLSCTLVINDRV
                     FKPFLDEMLETLKNIDDPQLKGMRLSEMQTHGQRVKCLVSPYHTPALNGLAAVNIRAM
                     YGLAKETWNHADWNRFYQSILCYSHYSQQALAHFGSAKVVGNPRFDAWHNGTFDRALP
                     ENIQSDYRKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLICKLHHGTCSRPEEAASL
                     ALVRRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMNDLLD
                     GEKSYSTAESADQRIREILPVAHDVAELRYLLSEAFDWGSVQARLTEIRHHYCDAFMD
                     GKAGERAAIVIVEALG"
     gene            complement(37499..37894)
                     /locus_tag="ACHKIK_000983"
     CDS             complement(37499..37894)
                     /locus_tag="ACHKIK_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFO9082199.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARALGERLLVGVSSDALNIAK
                     KGRAPVYHQDDRMAIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDS
                     FAYICEVVYFPRTPSVSTTGIIEVIRGLL"
     gene            complement(37897..38331)
                     /gene="fliJ"
                     /locus_tag="ACHKIK_000984"
     CDS             complement(37897..38331)
                     /gene="fliJ"
                     /locus_tag="ACHKIK_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO9082200.1"
                     /translation="MRQIIDTLTQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAVSVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            complement(38334..39671)
                     /gene="fliI"
                     /locus_tag="ACHKIK_000985"
     CDS             complement(38334..39671)
                     /gene="fliI"
                     /locus_tag="ACHKIK_000985"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO9082201.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESAGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTSLEHQQSARALKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPGVKKAEQ"
     gene            complement(39664..40374)
                     /gene="fliH"
                     /locus_tag="ACHKIK_000986"
     CDS             complement(39664..40374)
                     /gene="fliH"
                     /locus_tag="ACHKIK_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO9082202.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTTSISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPA
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALKETLTPESQGE"
     gene            complement(40377..41387)
                     /locus_tag="ACHKIK_000987"
     CDS             complement(40377..41387)
                     /locus_tag="ACHKIK_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO9082203.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVGRLS
                     ENMARLSGVKTSMARKVINNFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLEQLQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAEQT
                     AE"
     gene            complement(41365..43011)
                     /gene="fliF"
                     /locus_tag="ACHKIK_000988"
     CDS             complement(41365..43011)
                     /gene="fliF"
                     /locus_tag="ACHKIK_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO9082204.1"
                     /translation="MNAQIKKLTLAFPAFRLRLADNKRWALMAGVGLAVAATAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEEALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLHASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTHASVANVLDSLVGSGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPVAANQMTNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTAQ
                     LTALLNNAAGIDAQRGDNLTLSLLNFVPQAVPVEPIIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPVMKRLTAVRAPIITPESEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            complement(43016..43357)
                     /locus_tag="ACHKIK_000989"
     CDS             complement(43016..43357)
                     /locus_tag="ACHKIK_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO9082205.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPAMTFSSTDPDVSF
                     HRIMQGALGHVDQFQQVAEQQQTAIDMGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            complement(43372..44358)
                     /locus_tag="ACHKIK_000990"
     CDS             complement(43372..44358)
                     /locus_tag="ACHKIK_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFO9082206.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENENAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQEVAAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYSTVLVKNITLSESTRRAMLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPENDIADHSELQWPAQPAVHIAETSDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKHGIEVFS"
     gene            44745..45596
                     /locus_tag="ACHKIK_000991"
     CDS             44745..45596
                     /locus_tag="ACHKIK_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO9082207.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKAHNGLPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESCFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLTQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENSHE"
     gene            45589..45960
                     /locus_tag="ACHKIK_000992"
     CDS             45589..45960
                     /locus_tag="ACHKIK_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO9082208.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            45957..46709
                     /gene="fliP"
                     /locus_tag="ACHKIK_000993"
     CDS             45957..46709
                     /gene="fliP"
                     /locus_tag="ACHKIK_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO9082209.1"
                     /translation="MKRRTQLTLGLGLLALAPVALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTML
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GHAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            46712..46984
                     /locus_tag="ACHKIK_000994"
     CDS             46712..46984
                     /locus_tag="ACHKIK_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HFO9082210.1"
                     /translation="MLTIDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            46986..47768
                     /gene="fliR"
                     /locus_tag="ACHKIK_000995"
     CDS             46986..47768
                     /gene="fliR"
                     /locus_tag="ACHKIK_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO9082211.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYVPVLDNSALTVRVRI
                     ILSLALAIIITPLIPHPVPHDLISLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            47758..48897
                     /gene="flhB"
                     /locus_tag="ACHKIK_000996"
     CDS             47758..48897
                     /gene="flhB"
                     /locus_tag="ACHKIK_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HFO9082212.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPEVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIERLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFASFLGLQALPFKLAMNEGLSLYACVMRNFVILFIFFALIDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDSSRAAAPFVVAKGTDEIALYIRQVAAENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            48881..50974
                     /gene="flhA"
                     /locus_tag="ACHKIK_000997"
     CDS             48881..50974
                     /gene="flhA"
                     /locus_tag="ACHKIK_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO9082213.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGADAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RHQLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLASMAPRLAEDLSAVLNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNLVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALS"
     gene            51079..51357
                     /locus_tag="ACHKIK_000998"
     CDS             51079..51357
                     /locus_tag="ACHKIK_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO9082214.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            51350..51706
                     /locus_tag="ACHKIK_000999"
     CDS             51350..51706
                     /locus_tag="ACHKIK_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO9082215.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(51763..52536)
                     /gene="yafL"
                     /locus_tag="ACHKIK_001000"
     CDS             complement(51763..52536)
                     /gene="yafL"
                     /locus_tag="ACHKIK_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO9082216.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSQLAEPFWQDHFLGARRILTEETIL"
     gene            52722..>52930
                     /gene="dinJ"
                     /locus_tag="ACHKIK_001001"
     CDS             52722..>52930
                     /gene="dinJ"
                     /locus_tag="ACHKIK_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO9082217.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGV"
BASE COUNT        11716 a        14554 c        13858 g        12802 t
ORIGIN      
        1 ctccattcag ggagagtgaa tggaggatta catactgcaa catcaaaatt tccttgatgt
       61 cgggcaagca tttcaggaaa tgaatgaaca agggcatctg ttacaatatg agtaagatta
      121 tcacatactt tttttgacat ccgagattct atatccaaag aatatgcttc tgcattcttc
      181 catcggtcta aagcagcata ggttaagctc ccctcaccag aagctaaatc aataatatta
      241 ttgacttttt cagctttcat ctgagaaacc aataatgcac ttatggattc ttttgtatag
      301 tatcttccga gactatctcg ttgtgtgaat ggcatatagt tactgttcat tttttatact
      361 catcttaagt acgctaccgc cctaggcgtt acggctaccc aagaaccaac acaatcatgt
      421 aatctattga tatttataaa cttatcttca aatctttcag ccctagaacc taaaaaagat
      481 agaagtgtag gtaacaaagg gcgatcggat atagggctaa tgtttaaact taagtatcaa
      541 aatatacggt tttaggtcat tttcctaatc aatcctatca tcacgtcaaa tctactttgt
      601 agcgttcacc agcaaagaaa tacatgaatt atgattagcg tttatatagt ccgcatacca
      661 ctgcatcatc tccctacgtc cttccaaata caacgcatgg ttatacgtcc cacgaatcgc
      721 atttttatca acgtgagcca gctgggtttc aatccacgct gtattgaaac cttcctcatg
      781 caaaatcgta ctcatcgtgt gacggaaacc atgccccgtt accctccccg tatatccaat
      841 ccgcttaaaa acctgattaa tactcgcttc actcatcgtc ttgcggggat cattacgtcc
      901 cgggaacacc agtggatatt gccctgacat cactttgagt tgctgtacga tttccagcgc
      961 ttgggtagag agggggacaa ggtgaggccg tttcatcttc atacgctcgg caggtatctc
     1021 ccacaccgct ttttcaagat caaactcact ccagaaagca cctcggagtt cgccagtgcg
     1081 aactcccgtg aggatcagca gacgtgccgc aagaacaact aacgggcttc ctgtgtagcc
     1141 tgcgagagct ttaaaaaagt ctggtaactc ctcaacagta aggaagggat aatgcttcga
     1201 ttcatgccct gacatcgcgc tggtaagatc cgctgcagga ttgtattccg cacgaccggt
     1261 tacgatggcg taacgaaaga cttcgctgca acgctggcga accttcttgg ccttctctgt
     1321 cgcgccacgg ctttcaattc gacgcagcac attaagcaga accagcggtt tgatttcatt
     1381 caccggttgt tggccaatat agggaaaaat atctttatta aaggcttcga taatgtccga
     1441 ggcataacct tctgaccatc ggctcacctt cgttccatgc cattcaagtg ccacagcctg
     1501 aaacgtatta tttagctgca catctcgaac cagcttttct tctttcttgg caagcgatgg
     1561 atcgatacct tcggcaagct ttttcttggc gtcgtcacgc agtgccctcg cttgtgcaag
     1621 ggacactgcc ggatagacac caaaggccat acgcttctct tttccattga agcgatattt
     1681 catccgccag tatctggaac cagagggaac aacctcaaga tacaaaccag caccatctgc
     1741 cagcttatag gctttctctc tgggtttagc tgcgtctacc tgtcgcgcat taagtttcat
     1801 tgggggcatc tccctagacc gaacacaaaa tgcccccact tatgccccca actgcgactt
     1861 gatttcggtt gagtctagtt gataacagga gataagatac gggccagaaa ccgcagtata
     1921 cgagctttta gttgatttcg gtagacttgg gaagagtttg aaatggtgcc gataatagga
     1981 gtcgaaccta cgaccttcgc attacgaatg cgctgctcta ccaactgagc tatatcggcc
     2041 ctgaaaggac atgttcacga acgtgaatca cggtggacaa ggttaaaact aaccgggcga
     2101 tgcgtcaatg gccttgtgaa tcaaatggct acttttgcat cacccggttt tatttacgca
     2161 cgaatggtgt aatcaccaat gccgatccac ttgtaagtgg tcagtgcttc cagccccatt
     2221 gggccacgcg cgtggagttt ttgtgtgctt accgccactt ccgcacccag accaaactgg
     2281 ccgccgtcgg taaaacgcgt agaggcgtta acgtaaacag cggacgaatc cacttcgtta
     2341 acaaaacgct gggcgttgcg catatcgcgg gtcaggatcg catcggagtg ttgtgtgccg
     2401 tgttcacgaa tatgggcgat ggcatcgtca agatcgctga cgattttgac gttcaaatct
     2461 aatgacagaa actcatcgtc atactcttcg gctttaacag caaccacctt cgcagggcct
     2521 gcctgcaact gcgccagtgc agctgcatct gcgtgtaatg tcacgccgct ttccgccatt
     2581 tgtttgctta atgcgggcag gaagctatcg gcgatgtttt tattcaccag caacgtttca
     2641 accgtattac atgtgctcgg acgctgagtt ttcgcgttga cgatcacttt taatgcttca
     2701 gcgatctcta cactttcatc aacgtaaata tggcatacgc ctataccacc tgtgatcacc
     2761 gggattgtcg actgttcacg gcacagttta tgcaaaccag cgccaccacg cgggatcagc
     2821 atgtcgatgt atttatccat acgcagcatt tcactgacca gcgcacggtc aggattatca
     2881 atcgcctgca cggcacccgc cggtaagccg caggatttca gggcgtcctg aatcaccgcc
     2941 accgttgcag cgttagtgcg acacgtttct ttgccaccgc gcaggatcac cgcattaccg
     3001 gttttcaggc acagcgaagc gacatcaacc gtcacgttcg ggcgcgcttc ataaatcacg
     3061 ccaataaccc ccagcggtac gcgacgacgc tcaagacgca ggccgctgtc cagtacgctg
     3121 ccatcgatta cctgccccac cggatcggcg aggttacaca cctggcgcac atcatcggca
     3181 atgcctttca gccgtgcggg cgtcagtgcc agacggtcaa gcatcgcttc gccaaggcca
     3241 ttggcacgcg cgtcagcaac atcctgggcg ttagcgttga ggatgatttc gctttgtgct
     3301 tccagttcat cggcgatttt ttccagcacg cgattttttt cgcggctgga gagttgcgct
     3361 aatttatacg aggcttgctt cgcggcaatg cccatttgtt ccagcatcag cctgctcctt
     3421 aacgggtaat catgtcatca cggtgaacgg caaccgggcc gtattcatat cccagtattg
     3481 catcaatttc ttgcgagtgg tgcccggcaa tacggcgtaa tgcatcgctg ttgtaacgac
     3541 tgacgccgtg ggcgatatcg cgaccttcga ggttgcaaat gcggatgact tcaccacgcg
     3601 agaaattgcc agtcacgctt ttaatgcctt tcggcaacag ggagctgccg cgttcaagaa
     3661 tggcggcagt tgccccttca tctaccgtga tttcacccgc cggcggcgca ccgaaaatcc
     3721 agcgtttacg gttttcaagc ggagtcgcct gggcatggaa cagcgtaccg acggaaatgc
     3781 cttccatcac atcaccaata acgcccggct tgctgcccgc ggcaataatg gtgtcgatac
     3841 ccgcacggca agccacgtca gcagcctgca atttggtact catgccgcca gttccgaggc
     3901 ctgaaacgct gtcaccggca atcgcgcgca gtgcgtcatc aatgccgtaa acatctttaa
     3961 tcagttctgc ctgcggattg ctgcgcgggt cagcggtata caaacctttt tgatcggtca
     4021 gcagcaacag tttatcggca cccgccagaa tcgccgccag cgcagaaagg ttatcgttat
     4081 cgccgacttt aatctctgcc gtagcgacag catcgttctc attgattacc ggaacgatat
     4141 tgttatcgag caacgcacgc agggtgtcgc gggcgttcag gaagcgttca cggtcttcca
     4201 tatcagcacg ggtcagcagc atttgcccga cgtgaatgcc ataaatcgaa aacagctgtt
     4261 cccacagttg aatcagtcga ctctgcccta ccgctgccag cagttgtttc gaggcgatag
     4321 tcgctggcag ttccgggtaa cccaggtgct cacgtccggc ggcgatcgcg cccgacgtca
     4381 caataacaat ccgatgcccg gcggcatgta actgcgcgca ctggcgaaca agttcaacga
     4441 tatgggcacg gttcagacgg cgcgatccgc ctgttagcac actggtgccg agttttacca
     4501 ccagcgtctg gctgtcactc atgattctct gccattcaat tttaggaaaa atgatatcaa
     4561 acgaacgttt tagcaggact gtcgtcggtt gccaaccatc tgcgagcaaa gcatggcgtt
     4621 ttgttgcgcg ggatcagcaa gcctagcggc agttgtttac gcttttatta cagatttaat
     4681 aaattaccac attttaagaa tattattaat ctgtaatata tctttaacaa tctcaggtta
     4741 aaaactttcc tgttttcaac ggggctctcc cgctgaatat tcgcgcgtta attaaaatca
     4801 ggaatgaaaa tgaaaaagag cactctggca ttagtggtga tgggcattgt ggcatctgca
     4861 tctgtacagg ctgcagaaat atataataaa gacggtaata aactggatgt ctatggcaaa
     4921 gttaaagcca tgcattatat gagtgataac gccagtaaag atggcgacca gagttatatc
     4981 cgttttggtt ttaaaggtga aacacaaatt aacgatcaac tgactggtta tggtcggtgg
     5041 gaagcggagt ttgccggaaa taaagcggag agtgataccg cacagcaaaa aacgcgtctc
     5101 gcttttgccg ggttgaaata taaagatttg ggttctttcg attatggtcg taacctgggc
     5161 gcgttgtatg acgtggaagc ctggaccgat atgttcccgg aatttggtgg cgattcctcg
     5221 gcgcagaccg acaactttat gaccaaacgc gccagcggtc tggcgacgta tcggaacacc
     5281 gacttcttcg gcgttatcga tggcctgaac ttaaccctgc aatatcaagg gaaaaacgaa
     5341 aaccgcgacg ttaaaaagca aaacggcgat ggcttcggca cgtcattgac atatgacttt
     5401 ggcggcagcg atttcgccat tagtggggcc tataccaact cagatcgcac caacgagcag
     5461 aacctgcaaa gccgtggcac aggcaagcgt gcagaagcat gggctacagg tctgaaatac
     5521 gatgccaata atttttatct ggcaactttt tattctgaaa cacgcaaaat gacgccaata
     5581 actggcggct ttgccaataa gacacagaac tttgaagcgg tcgctcaata ccagtttgac
     5641 tttggtctgc gtccatcgct gggttatgtc ttatcgaaag ggaaagatat tgaaggtatc
     5701 ggtgatgaag atctggtcaa ttatatcgat gtcggggcta catattattt caacaaaaat
     5761 atgtcagcgt ttgttgatta taaaatcaac caactggata gcgataacaa attgaatatt
     5821 aataatgatg atattgtcgc ggttggcatg acctatcagt tttaatgatt attgccggat
     5881 gtgatgcatc cggcacattt cactcacgcc gttaacttca ccggctcgtc acgaaaatca
     5941 tccgccggtt ccagcttcag attcagcgtc gtcagcagtt cacgcagctt ctcgtgaaac
     6001 tcacgcaggg tgtgctccag tcgttcaacc acttcagtgt cttttaccgg aacactcttc
     6061 cagtcgcctg ctttatcgaa cagaccaaac tggtaactgt aggtaaaacg ggattcctgc
     6121 gcttcaagct ccatccacca gccccagaac tcacgcactt ccggtgccgg tttcacgttg
     6181 acgcatacag ccagacaatc gaaaaagaat cgattatctt cgcacttacc ttcacgaata
     6241 tacgggccta gtgcggtaaa ttttttgatc aatctgctct tcgggtgtcc actcggtaac
     6301 gtcattgcta tctcctgttg tgatgcaact gttttaccaa attggcaaaa tttagcaaat
     6361 ttttaacaca agcgtttttc gatccagccg gtgatttcct gaagaccttt gtcaaaattc
     6421 cgatacaccg ggttaaatgg gatctctaat aatttaccgt cagcagatga tgaggtgatt
     6481 aagcgtgagt cctcttccgg gctgaacgga tcgttcttcc agtagcctga taacattggc
     6541 gttgggcagc gacgccccag caatccttgc acttttaatg aatagcgatt cagctccacg
     6601 cgtaacgcgt catcggaagc atcatgcatc cccaaacgac tcgccagaac gtcaagatac
     6661 atttccggca cctgttgctg gcacttaaaa tcactcaaca gggtatgaac taccggacca
     6721 agacaggcaa ccgctttcag acgcggcgat tcaagatatg ccagacgcac ggcaacgtta
     6781 gcgccgaaac ggaaaccaaa ggccgcgacg cgagtgtgat ccacccacgg tacgttaggc
     6841 agcgccttta agacgtgctg atgcaacagg ctggagtcct gggtgagctt ccattttgaa
     6901 gaaaagccca ccgatggcat atcaatagtc agcatcgcaa tgccgcgcgg cgcaaaataa
     6961 cgttcataca ggctgtaata gtccgtctgc atcgcatcca gaccaccaca cattaatact
     7021 gtcgggaacg ggccatcgcc tttcggcata tgcaaaaagc cggtgatggg cgcaccgccg
     7081 ggtacggtaa actccatctg ccgcatcgtg cccggcagac gctgggcggc ctcttcatag
     7141 gcgcggtttg ataaagcctg cgcttgctcg gccaggtcat ctcctttcag atgaggatag
     7201 gcggcaatgt tgtacaacgt agccgcatgc agccagtgtc gaccactgag ttgcggatct
     7261 tcttccgcac aggctttttg ttgccacacc atcgcctggg tggcccactc ataaatccag
     7321 ttgccaccac ggtagccaat cacagtatcg tataaatcat cgtcggtacg ttcagcatcg
     7381 ctcatcacaa tacgtgcctg gacgtcgagg atttcacgtg ggtcaatacc gcgccagatc
     7441 cacatcagac ggttaatcat gcgataccag tgagggatgg ttttaccatc aagagccgac
     7501 tgcacaggcg gttgtgcgcc gtgattaaag cggcggacta gcgtcgaggt ttcaggatgt
     7561 ttaaagcggg gtttgaacag ggtttcgctc aggtttgcct gtgtcatgga tgcagcctcc
     7621 agaatactta ctggaaacta ttgtaacctg cctgaagtta aaaagaacaa cgcccggcag
     7681 tgccaggcgt tgaaaagatt agcgaccgga gattggcggg acgaatacga cgcccatatc
     7741 ccacggctgt tcgatccagg tatcttgcgg gatatcaaca acatagtcat caaccagcgg
     7801 acgaccagcc ggttttgcga agatggtgac aaagtgcgct tttggataca tttcacgaat
     7861 cgcaacagca gtaccaccgg tatccaccag gtcatcaata acgatgaagc cttcgccatc
     7921 gccttctgcg cgtttcagca ctttaagctc gcgctggttg tcgtgatcgt agctggaaat
     7981 acaaacggta tcgacatgac gaatacccag ttcacgcgcc agtaacgcac ccggtaccag
     8041 accgccacgg cttacggcaa taatgccttt ccattgttca gaaggcatca gtcggcttgc
     8101 gagtttacgt gcatggatct gcaacatgtc ccaggtgacg atgtattttt cgctcatgtg
     8161 aagtgtccca gcctgtttat ctacggctta aaaagtgttc gaggggaaaa taggttgcgc
     8221 gagattatag agatctggcg cactaaaaac cagtatttca catgagtccg cgtcttttta
     8281 cgcactgcct ctccctgacg cgggataaag tggtattctc aaacatatct cgcaagcctg
     8341 tcttgtgttg acaacatttt ctgctaaccc tgtgacctgc aatactgttt tgcgggtgat
     8401 cgacaaggag acttaacgtg tctgaactgt ctcaattatc tccacagccg ctgtgggata
     8461 tttttgccaa aatctgttct attcctcacc cgtcctatca tgaagagcaa ctcgctgaat
     8521 acattgttgg ttgggcaaaa gagaaaggtt tccatgtcga acgcgatcag gtaggtaata
     8581 tcctgattcg taaacctgct accgcaggta tggaaaatcg taaaccggtc gtcttacagg
     8641 cccacctcga tatggtgccg cagaaaaata acgacaccgt gcatgacttc acgaaagatc
     8701 ctatccagcc ttatattgat ggcgaatggg ttaaagcgcg cggcaccacg ctgggtgcag
     8761 ataacggcat tggtatggcg tctgcgctgg cggttctggc tgacgaaaac gtggttcacg
     8821 gcccgctgga agtgctgctg accatgaccg aagaagccgg tatggacggt gcgttcggct
     8881 tacagagcaa ctggttgcag gctgatattc tgattaacac cgactccgaa gaagaaggtg
     8941 aaatctacat gggttgtgcg gggggtatcg acttcacctc caacctgcat ttagatcgtg
     9001 aagcggttcc agctggtttt gaaaccttca agttaacctt aaaaggtctg aaaggcggtc
     9061 actccggcgg tgaaatccac gttggcctgg gtaatgccaa caaactactg gtgcgcttcc
     9121 tggcgggtca tgcggaagaa ctggatctgc gccttatcga tttcaacggc ggcacactgc
     9181 gtaacgccat cccgcgtgaa gcctttgcga ccattgctgt cgcagctgat aaagtcgacg
     9241 tcctgaaatc tctggtgaat acctatcagg agatcctgaa aaacgagctg gcagagaaag
     9301 agaaaaatct ggccttgttg ctggactctg tagcgaacga taaagctgcc ctgattgcga
     9361 aatctcgcga tacctttatt cgtctgctga acgccacccc gaacggtgtg atccgcaatt
     9421 ccgacgtggc aaaaggtgtg gtcgaaacct ccctgaacgt cggtgtggtg accatgactg
     9481 acaataacgt agaaattcac tgcctgatcc gttcactgat cgacagcggt aaagactacg
     9541 tggtgagcat gctggattcg ctgggtaaac tagctggcgc gaaaaccgaa gcgaaaggcg
     9601 catatcctgg ctggcagccg gacgctaatt ctccggtgat gcatctggta cgtgaaacct
     9661 atcagcgtct gttcaacaaa acaccgaaca tccagattat ccacgcgggc ctggaatgtg
     9721 gtctgttcaa aaaaccgtat ccggaaatgg acatggtttc tatcgggcca actatcaccg
     9781 gtccacactc tccggatgag caagttcaca tcgaaagcgt aggtcattac tggacgctgc
     9841 tgactgaact gctgaaagaa attccggcga agtaattatt tgatttgctg ccggatggcg
     9901 tttaatcgcc ttccggcagt ttcatccttc attatccttc gataaaagcc atccctgtaa
     9961 atgtccgtct cgggttgcca cgttcaatct ggtgatggaa cattcgccgc agcgatttca
    10021 gcaccgcgct attttcctgt tgctgctgct ccagcttcca ggcaatcagc aatcgtgcca
    10081 gccgcttgtt agcatgctga ctacgctctg actgaacctt cacgctaata ccggatgcca
    10141 aatgcgtggc gcgtaccgcc gagtcggttt tattgacatg ttgaccgccc ggccccgacg
    10201 aacgcaacgt ctcataacgg attgcatccg attgttcctg ctcatcagcg gtaaaacgcc
    10261 caatgcccag aaaccagttt ttgcgcccat gatgaggccg atacggactc ggacaaatcc
    10321 actgaatagt gccacaccag ctttcgctta atgcccatgc gttatcgcca tccagagaaa
    10381 tcagcgccga acgcagcgtg tcagagtagt ggcccgtttc tgtttccagc accgttaccg
    10441 cgacgtcttg tcgggcagct tctttaatta gcctgtccag cgcctttttc actgcgagac
    10501 aacattcttc cggcccctgc gctgaggaga gttgtagcaa gatcatcctt ttttccctcc
    10561 actgtttttg agcgttagca ccggacgcag ccgcgcgaca ggaataatta aaccggcctg
    10621 caccagacat tgcaccacgc tttcagccga tttataggct tgtggcgctt cttcaaagat
    10681 gagttgttta tcgcgacaaa ttacccgact gccaagttca gtacgcgaga gttgcgtcgc
    10741 cgtgtatttc gctgccagac gccctttaca ctcggtgcgc ccccatttac gccctgcccc
    10801 atgcgccagc gagtggagcg ttttttcgtt cgcaacaggc tgtacaatcc aggagtaatc
    10861 accacgtgaa ccgggaataa tcaccagacc gttgtcatcc ggcgtggcac ctttacgatg
    10921 caaccagccc tgttgatcgc cgatttgaca cgcgctaaca aagttatgcg ccacatccag
    10981 aaccggactt cccgtggcct taacttgttg cattatgcgc aaagcgatca tctggcgatt
    11041 aatacacgca aacgccagcg catcgtcatg ttccgcaata taccgtagcg cgtcgtcact
    11101 gccttgaggc aaaccatgat gcgagaacga ggcaatatgc cgctgtaaaa tagactgccc
    11161 caccccccgc gagccgctat gaaccaacag ttgcagatgt tgtgcatcca gacctgccag
    11221 tgcaaacagt tcagcgttga tgatttgatc aacttgttgc agttcagcga aatgattgcc
    11281 gccaccgata gagccaagcg agctgcgcca cggatgctgt gcaaatgctg acggcaggtt
    11341 ttcctccagc cagctttctt cagcaacgtc atccagatct gataatcgct tttcaaactt
    11401 atcggcgttg tatttgcgag cgagaatatc tgtttgccat agcgccatac cgcagccgat
    11461 atcattgccg accagtgccg gataaaaacg accaacagag aagaacgctg cgccaatcgg
    11521 atagccgcgt ccgggatgta aatctggcat ccccactacg cgctgcatgt tgggtaaatt
    11581 tgccgtggtg tgtaattgtt ggatcgctaa actctcgatc cacaggtcat cagatgcgat
    11641 ggtaaatacc gcatcggata agggacgaat ataattgccc atataccatt ccgtaaatag
    11701 actcgtcata cttcaagttg catgtgttgc gacttgaatt attgagagta acaattaaaa
    11761 aatcaggaaa tggcagacga atccagggca agaaaaaaag aattaatcct ggaaaggtct
    11821 gcaaagagtc attgataaag gatttgtcat gatttacctc cttgcagttt gtggaataag
    11881 aaaacgcgca catactaggc ttgcgttatt ttcattgcaa gctggattta atgttgctgt
    11941 ttataacgca tataaaaatt aataaaccac tctccccgac attcaacgcg ttgctgctta
    12001 actgtctgaa aaccataacg ttcaaaaaag ggttttgcgg ttatgcttgc gtccaccgta
    12061 agttcggatt cagacttaat caaaggtttt aacaaagcgc tggcaacccc acggcgggtg
    12121 tattcagggt caacaaataa catatcgata tagtgttcaa cgcaggtaat aaagccgacc
    12181 agttttgcat taatgactgc aacccgcact tgtgatttcg cgagtttctc cttccagcga
    12241 gattcgtcaa tctgcgccca ggcggcaatt tgttgtggtg aataatgctg actggcagtc
    12301 atcgtaaccg ctctaatgaa aatagcgcat agttgctgaa aatcgccagg ctgatagtct
    12361 cttatttgta tgttattcat aatcccagca ccagttgtct ttccatttgc gggtcaagca
    12421 acgtcacatg cagccccacc agtcgcacac cgcgcccgcc gcggcgttca tcccaggttt
    12481 tacgcgcggt ggcgattaag tcagctttat tcagccgcgg ccagacgtgc tcctgagtgg
    12541 tttgctgaaa atcatcaaac tttaatttca ctccctgacg ggcgatcagt aaatcaggtt
    12601 ttacctttgc cagacggcgt tcaagttccg gatacagccg ctcgataatc gcttcacatt
    12661 cagaccagtg atgaatatct tccgccatcg tgcgttccac gccgacggat tttcgtaacc
    12721 gttcgctgtt aacgtcgcgc tcgtcaatcc cctgactacg ctcccacaaa atgcggccaa
    12781 atttgccaaa gcgtttaagc agcatcacca gatcacactt ttgtacatca ccgcaggtcc
    12841 gcagccccat cgcttccagt tttgccgccg agactttgcc gacgccgggg atttttgcca
    12901 gcggtaaggt ttgtaaaaat gccggaactt ctgccggcgt aatcacaaac tgaccgttgg
    12961 gtttattcat gtcggaggcg attttggcga gaaactttac tggtgccacg cccgcagacg
    13021 ctgtcagttg cagctcgctg aaaatcgtct ggcggatttc ctgggcgatg agggtcgcag
    13081 aaccgtggca atggacgcta tcggtgacgt cgagataagc ctcatccagt gacaacggtt
    13141 caatacgcga ggtgtagcgc gagaagattt cacggatatg atttgaggct tctttgtagg
    13201 cgtcaaagcg ccccggaagc aaggtgagat gcgggcataa tttgagcgcc atccctgtcg
    13261 gcatagcgct acgtacgcca aatttacgcg cgggataatt ggcggtgctg atcacccccc
    13321 gacgttcgcg gctgccgcca atagcaatag ggatatcgcg cagggcggga ttgtcgcgca
    13381 tctccaccgc tgcgaaaaag cagtccatat ccacatgaat gattttacgc attactcacc
    13441 tctcaacact ggtaaagtat acagtgattt cagggtttga gaaatgcgta aagattcagc
    13501 atttaacgcg ttcgctgcga aagcacctgc tgcttatcca gcttttgcac cagcggctgc
    13561 accactttat ccccatgctg accaaagtat tgatacagcg tatcggacgc acttttggtc
    13621 agcaccataa tctcaatgcg ccggttgccc gcgctttgcg gatttttgga atccagcagc
    13681 atctggtccg ccattgcgct tacctgcatc actttatctt ccggcattcc ggcctcttcc
    13741 agcacccgac gagccgaaag cgcgcggtca cccgaaaggt tccagttgtt gtagatattg
    13801 tttttgtaag ccatcgcatc ggtatgcccg gtaataataa ttttgttatc gagcgagtcg
    13861 aaaactggcg cgagctccac cagcagcgtt ttaaagaacg gcataatctg cgcgctgccg
    13921 cgttcgaaca tattgcgatt ctggtcgtct ttaatcagta cgcgtaatcc ctggggaaca
    13981 atctccatct ccagattcgc ttccatatgc gcatcacggg caatggtgtt gatactggtt
    14041 gccagctcgc caagctcggt ggcggatttc ttcttcagca gcgccgtttt ctcattcaca
    14101 tcacgcgctt ttttctccgt ctcttccggc accgccacgg tggcaggttt tggcggatga
    14161 gacgggctga ttttatttaa cggcgacagc ccgccaccgt taaaaatcga ctgcccgtgc
    14221 agcgcggcga taatgctttc acgctcggat ttactgacgc tgttgacaat ccacagcgtc
    14281 ataaacagcg ccatcatcgc cagggtaaag tcggcgaacg ccaccttcca cgccccgcca
    14341 tgatgcccgg catgattctt tttgatctgc cgccggacga tggtggtttt tgccccacgc
    14401 tcgcggcggt tgcccgtctt tctcatgctt attcttgctc cagcatcgca ttcacccacg
    14461 cgtcgaggtt ggcgaaggtc ggtttggacg ccagatgcag cagcttacgc ccggcatcca
    14521 ccgccagcag cgtcggtttg ccgcctgcct gcgccaccag cacagtgcgc acgcattcca
    14581 gcaaagaatg ttctgcccgc gcctgttgtt ccatggcgtt ggcgagcggg tccatcaggc
    14641 agtagcagat aaagacgcct aaaaacgtcc ccaccagcgc tgccgccact ttcaggccga
    14701 tcagggcaat agagccgtca atcgactgca tggtgatgat gatccccagc actgctgcac
    14761 aaataccaaa tcccggcatc gcttcggcgg tacgttgcag cgagcgcgac ggcgtgagca
    14821 gcgactcttc cgcagtgtcc agttcctgat cgagaatgcc ttccagttca tgggcgtcga
    14881 ttttacccat cgccatcagg cgaaagttat cggcaataaa ggtcaccagc cgcttctggg
    14941 ttaacaccag cgggtatttc tggaaaatgg tgctctcttc cggttgctca atatgttgat
    15001 ccagcatgcg cagaccaccg ttttgcacca tttcgagcaa ctcatacagg cacattaata
    15061 gctgacgctg aaactccggc ccgagctgtt ttttactgat cacaccttta atttgatggg
    15121 caatctcttt cagcacatgt ttcgggttac caataatcat cgccccgaaa ccggcaccca
    15181 aaataatgat catctccgcc ggttgccaga tagccactaa ttgcccgccc gcttcaaaat
    15241 aaccgccaaa tacgcagccg agcaccacca gtaaacctaa tattttttgc atgatttctt
    15301 cactttaatt cggatagaca aaatcacgaa ttttctgcgc gatctgttta ttcagttgac
    15361 aaattcgggc ctcggttagc cctaatacca gcgcaatctc tttgagattc atctcatgct
    15421 ggtaatacat ggagaggatc agttgctctt tttcgcttaa ttgtgaaagc gcctgttgca
    15481 gcatttgctg agtgacaaac tgatcttcca gctcccggcc aacaagcgga atgcctggcc
    15541 cttcgccgtt cagcagttcg tccaggctgg cgagcgtttc cgccccttcc agttgctgat
    15601 attcgagata atcttcatgt gagatccctt ccacggccag ttctgaccac tccggttcgt
    15661 gccccagttt tttgcgcgtt tcgcgaatca gatctttcat ctgatgatat ttctgtcgca
    15721 gctggcgcgg tcgccagtcg agagcgcgca gctcatccag gatcgcgccg cgaatacggt
    15781 gcacggcata gcccgcaaaa ccttcgtccg gcagcccgta gcggcggatg gcattaagca
    15841 gccccattaa cgccgtttgt tccatatcct gacgatcaat aatgcaatta cactgcgggg
    15901 caagttgccg caccacttta cgcaccaacg gtaaataggc ctgcaagtag tggctctcct
    15961 gctgcggggt taataccggc gcggcagcaa attcttctga ctcgaataca tcctgtagca
    16021 tgtttatatc cgcctggtga ttactggaag accacttttt tcaccagcaa atccttatac
    16081 ggtgcgctca ttttgcgacg ctgaagatct ttctttaacg cgtcagaaat gagtttgcgg
    16141 atggcagata ttttcatgcc gcgcaaatct tcaaatttca tttccgtcag gcattcaacc
    16201 gcaatacttt gatacagcgg ctcctgatgt tttatttttt ccgcttcttt gtcactggcg
    16261 accaccatca ctaattccgc cgacaaataa tggtccgcgc cgttgttgtc atgcagcgtg
    16321 acaatggttt ccggcaacgt cacaaacacg cttttgcttt catccagggt ttccaccgtt
    16381 tccgtctgcg cggcggcagt cggttttcct tttccggcat aatgatgcca gacaccccat
    16441 ccagctccgg cccccaccac caacgccaga acggcgctga ccacgcccgc caccacgatt
    16501 ttcttcatat tgcctttaca ctttgatcaa aacggattcg ttctgttgaa attgaggcgc
    16561 atcatcaagc tgtaaggcgg cactcacctc ttcctgctga tggcccgact gttgttgttg
    16621 ctgcgactgc ccttcggagg aaacctgtac gttcacctcc ataaaatttt gcgccgtcag
    16681 atgctggcgg agatcgtcgc taaactgctg taacgcgcgg caaacttcac tttggttcgc
    16741 gccgatgtgc accatcagtt tgccgttgtc gagttgtacg gcgatctcga gcttgccaag
    16801 cgacggtgga tcgagacgga tggtggagat ctgctgttgt ttgttgagct gaaagtggat
    16861 ctgatctttc aaaagcgtcg tcagtttttc gcccagttcg tccatcgaaa gcgtggctgg
    16921 tttggcgaca atctcctgat gatcgctgag cagctccggc gtcttaacct gcaacaccgc
    16981 acgcgcaata accggttcac tgtccggcac tttttccgtt ttacgctcga cctgcgggcg
    17041 cgcggtcaca cgggttagtt cagatttcgg gcgagctggc tgtgttgaga cgcgcggctg
    17101 cgtcggcgta atggcatgta aatcttcact agcgtaagtc gccagtctgg cctgctgttc
    17161 gggcttcgcc tgcggcaact gactgatcag ttcctgcaac tgcggcggca acggcgtgag
    17221 ttcctgctgt tgcgtctggc gttgcggcgc gttttgcacc accgctttcg tcaattgctg
    17281 gataaccggc gatgttgcga cgtttcgcgg ctgcggcgtg tcctgatgcg gcgcggcagg
    17341 ttgcggaagt aacagcgcca ttagcgcctg catcgccacc ggatcgttct cctgcgggcg
    17401 cgtcgcctgt tgctgactgg tttttgcgct atgaacgcgc tccgccaccg ccgcgaccgc
    17461 cattttcggc agggtaaacg cgggcgcgtt ttcaccgctc accggcggta aaatatccgc
    17521 cttcaacgaa gcggtttccg caagcgttcc gagcgtggca agcaatgtcg gattcatgac
    17581 ttctccgaaa gtaaattaac aattgagtag gccagtacgc cttcctgatg ccgctgatgc
    17641 tgttccatac gcgctttcaa cacttctgcg cgctgctcgc gccgtttaat cagggcgtta
    17701 aacgcgtcat acagtcgcgc tttcactgca tccaactgcg ccgcatcggt ttccgccgcc
    17761 gcgtaatcgt gaaactggcg tatcaactgc tgatgcagtg ccggaaggcg tcgccagcgt
    17821 tgcttgtcga gggcttcatt catcgcctcg accagtgcga aaagtttcct gcgttgtttt
    17881 tccatcgtct taacccagct tgcccgaaag cccttcccag ccttcgcgca ggttgcttaa
    17941 gatcagcatc acttcatcga ttttctctgc cgacagttcg ccgctggcct catacaatcg
    18001 gtaaacgcag tggtcataga gtcgcgaaag gttgaccacc agctcgccgc cagtttcaaa
    18061 ctccagcgaa ctggtgagcg cattgagaat gtcgatgcat ttattgatgc tctgggcttt
    18121 cttctcgaaa cgccgccctt caatatggct tttggcgcgt tccagttcgt ccatcaggcc
    18181 agaaaacagc accagcacca gttccagagg cgatgccgcc gcagtacgcg ccgcgagatc
    18241 gatctcttta tattgcgaat agccgtcctg tacttggtac atctttaggg atattccttc
    18301 tgagttacgc gaatgccgcc atgctggctt tcatggtgta cacctcgacc agcgtgttgg
    18361 tgtactcctc gagataacgg tcgtagttgg cgttataggt atcagtaagc tgatcgcctt
    18421 cgtcctgaat tttgctcatc tgatcgtcaa tgttctgctg gcgcagggtg atgatgccgt
    18481 tgctggagtc cgtgtaggtg ttaataaggt cgtccatctg cgccaccatg ctgttgtcgc
    18541 caacgaaaat agaggtcaga ccgtcaggat ttttcgccat ttcgtcgttg aactggtcgg
    18601 agtcaatctg taagtggccg tgagaatcga gggtgatgcc atagtcaaca atcgacacgc
    18661 cgttgtagct ggcatgggcg atgtcatcga gctggttcgc cagtgaactg agtccggcgt
    18721 cgcctgcaaa taccccggcg ctggtactgt catcaccgtg ggtggttagc gaatcaaccg
    18781 tatcaatcaa ggtgttatag gcatcgacaa aggtctgtac tttctcctgg ctggcgctgg
    18841 aatcttcgct gatgttaaag gtagtgagat cgctgtcaga atcgctgact tcggtgaaag
    18901 tcatggtgac accgggaatc acatcatcaa acgtattgct gctgttggtg atctcaggcc
    18961 ccgtggcgct gccaagatga ataatcgcgt cctgagcgga ggaaacatcc gtggcaacgg
    19021 gcgcgctggt gctatcgttg ctggcatcat gccccgttac gctcaccgaa aacgcgctct
    19081 gcgccccggt gctatccgag gtgagcataa tcgttgtggt gccgtcggtt tttaccagcg
    19141 ccgccgacac gcccggatta tcatcggagt cgttaatcgc attcaccagt tcactggcgt
    19201 cgataaaacc gtcgccgtca ccattctgat ctgccgccga cagatcgata tccatggtgc
    19261 tgtcgcccat cgtcagttca aaagtgccgg tggcggaaaa ggcgtcgtcg cccatactga
    19321 acgtggtttg ctgcccctgt gccagttgct caacgaaaaa tgagtagctt cccgcctgcg
    19381 cctgggaatt ggcggaaacg ctcgccgagt cattattgct ggtggcggca aacgtcaccg
    19441 ggccgtcggt atcgctgttc agcgcgtcaa cggcgctctg aaaatcgctc aacgctgaac
    19501 tgagcgaatc caggccgctg ctttcagcat ccagctctgt ttgcttctcc tgcaagtttg
    19561 ccgcctgagt ggcgacatcc gcatacgcga tttcttgcgc aatggttctt gggttgatca
    19621 ttgatgactc cggataacga tgtcaggtta ttggcaaaag gcgtgccaat attttttatg
    19681 tgaataatca atagattaaa aaacaggcgg aaatggcgtt tccggtctgg cggaaaaaag
    19741 cctgccgcag aaagcacaac accgcccgca ggcggtgttg ttcgtagtcc gaataaggcg
    19801 tttacgccgc atccggcaag tccgccatta ctgcagcagg ctggaaacca tgctggacat
    19861 gctgttggac tgtttcagca tggtgatgcc agtctgcatc agcacctgct gtttggtcat
    19921 attggacgct tccgtcgcgt agtcggtatc cataatgtta ccgatagcga cttcggtgtt
    19981 gtcctgcata ctggtcaggt tgttggcggt gtcgttcaga cggttgatag acgcacccag
    20041 cttggactgg atctgcgaaa cgtcatccat cgcggtagag atgctggtga tcatctggtt
    20101 agccgagcca gacgcggtca gctcagtacc gccagaaaca cctgcagtac ccgtggtgtc
    20161 tgcctgatcc gcgctaaagg agttgctgat agcagaaaga tcggtcacca gcgtgttcag
    20221 ttggctggag atgttcacgg tcatggtgtc ggaagattcc gcgcccacct ggaaagttac
    20281 cgcgctctgg aacagaccgt cagtaccggt gttgctggta ccggaaacac cgaacaggtt
    20341 ggtgccgccg taagtggtgt tttgcagcat gtcagacatc tgctgaccca gttcgtcgta
    20401 ttcgtcctgc atcgcctgca gatcgtcgtc gctgtaggtg ccgttcgctg cctgagttga
    20461 caggtctttc atacgaccca gcacatcaga catttcatcg aacatactat cggcggtttg
    20521 cagcatcgcg gttgcgtcat tgatgttgct cagcgccacg cccataccgc tggactgtgc
    20581 tgtcagacgg ttagcaattt gtttgcctgc ggcgtcatcg gcagaagagt taatccggtt
    20641 acctgtcgcc aggcgttcca tagatgtaga caaagaagag ctgcttttgc tgattgcatt
    20701 aacggcggcc attgaggcgt tattggtgtt aatagataac atgatactgc tccttgggat
    20761 taatcgtttt cgtttcgata ccctgtaaaa acgacaccta aggagcaaac ttaaaaccgc
    20821 cgttaaaaaa atttcactgc gatttatctg atgccacttt tagctaaaaa aagcagagtt
    20881 taaattcaga taaaaagatt attagcaata tctgccacat ggccaggtat caaaatatgt
    20941 ctttatcaaa cgcttgatat agttaagatt accatcaatt aacgcgtttt cttctcaatg
    21001 tactgatgcc aaaatttaca ttgcgttgca aatagtacac atttggctat tgtaccagtc
    21061 ctgtcatttc gttacatttc catgccatat agtgattaag aataatccta ttaccataaa
    21121 atattagtta tcacgcagaa aaatcatctt ctcagggacg tataagactg tgctttatgc
    21181 aaagcactat caggaaacat ttatcccttg ctaaggtaaa ctcaactatg agtattatta
    21241 tcaacaactg gcggatggac ccgtcgctta atgccttaat acattgcgaa acgggtgaaa
    21301 cacgtcgtct cggcgaatac cattttattt tgctggaaaa attagcaaaa aatgccgatg
    21361 tggttttatc gcgatcttat ttatgtgccg aagtatggaa gaaccgcatt gtcggcggaa
    21421 acagcctgcc gacggcgatc catgcgttgc gcgttgctat cgatgatgat ggcaaacaac
    21481 agaacattat taaaacaatt ccgaagaaag gttacttatg caataaagag tatgtgtctt
    21541 tgccggactc atcgcctgca aaaaaactga ttattaccga ccagatacag gaaacagtac
    21601 cagaagagat atcttctaca acaccgcctg tgcctgttag aaaaaaacat aaagtaataa
    21661 tggggctagc gttagccgcg gctgttatat ttattggctc gaccgtgggg tattcacact
    21721 tgaaaagtac acctgatgcc ccgcaactgg taaaagagtc aattaacagt ccaagaataa
    21781 aaatatttca ccttagttct ggaaaagaaa ataattccgt accgttactg tcacaaaccc
    21841 tcgcaccagg aaaagacaaa ctcgataatt tattgtcagc gcataatatg acaatgacaa
    21901 cctattataa atatgttcgt aaccgactgg aaagtgatat tgttctgcgc aaccagtgca
    21961 atggtagctg gcaattaact tttaatgtgg aaggctggca gaacagcgat attaatagcg
    22021 cgatgtatca gaatttagag aagttgttaa atactttgca gaaatgctga tgagtgccaa
    22081 tatatgccgg atgcgacgct aaacgcgtct tatccggctg ataattaccc caataaattc
    22141 ctcaccgtct ggctggaaat tcgcgcctga ccattcaccg cctgcaataa aacctgatag
    22201 ttatgattcg cgctatccag cacgccgccg aggttttccg acgcctgcac cgcgttttcc
    22261 gtttgcggga agcgcgacat gccgtcgatc aactgccgcg ccttttcctg ctgtaccgcc
    22321 atctgcgcac gctgctgatt aatgctttct aacgtctgag aaattcccgc accgccctgt
    22381 tgcaggcttt gagctaaacg gtctgcctca gacggctcgg caaaagtttt cagcggcata
    22441 aacgccgact tatcgccatc ggtgcgcacg cttaaggtac gttcgatatg gggccactgt
    22501 ttttcggtgg tggagaaact gatgccgtcc gcttgctgat gtagctgcac gcccacgcgg
    22561 cgcagggcgt tggtcagccg cgtctgatac tgcgcggcgc tgtcatcctc agacagcatc
    22621 accgccgaaa gctgagtctg ccgcccgtca gagacgctga acatgcgcgt tcccggcgtg
    22681 ggattgtgta ccagattcgc cagatccggc gagtgaaagg tcacccgcgc ttcgccctgt
    22741 aacaccggct gcaactggcg gtccaccgca ccgccggaaa gcgcggtgcg tttgtccagc
    22801 atctgcatca gcgccgtatt ctgcgcctgc ccgcctttac gctggctgtg acgatagtcg
    22861 agcaactgct gctccagttg cccgagataa tgatccgcct gctgcaacgt ggtgagctgg
    22921 tcgttaagct gcacgctgta acgctgcggg cgagtggtaa tcagcggcga ggccggatat
    22981 tcactcgccg tcgccgggag tttttgccgc accgcctgag tgggcgcaac ggttgacgac
    23041 gataccgcag cgctatgcgc gccgccggtg gcgagtgatg aagatgtaag tccgacctgc
    23101 atactgttcc tgaaagagtt agagaacgct gaacaaattc aactcactca ctttcgagta
    23161 agtcttgagg gtaacttcca ttgccacttc cagtccggta aaggtgatcg acgccgggcc
    23221 gtaatcgaga tcctgtaatg aaccaatcag ctcatcatta gaggtggaga tatcggtctg
    23281 cgcatcgctc agcatcgaca tggtattttg cgtctcgccg agtgacgcaa tcgaggcgtt
    23341 gaggtcgtcc gaggcggtgt ccaccacatc aacggcattc tgaatatcgc tctgcacctg
    23401 cggatctgcc gggtcgacgt ccgggttttg caactcctgg gatagggaat tcagggtgtt
    23461 gagcacgtct aaattgctgc caaagaaatc actcgccgcc acgttggtgt ccacttccac
    23521 gccgttcgac accgtggtct ggcggtggtc gctgttaccc tgatagctgt agctgtcggt
    23581 gacgccatcg ccgtcatcgt ccactgccac aatcggcggc tgatcgttga tggtgccgcc
    23641 aaacacgtag tgcccgtctt cgtcctgata attgagcgcc gccaccattg attcggtgag
    23701 cgactcaata tcctgcccga ggcttgcgag tgaatcggcg gtattggtgc cattcgccgc
    23761 ttccagcagg tcgtcgcgca ccgccagcag gctgttgttg acgccatcaa ggatcgactc
    23821 ctgctggctt aagcccgccg acgcggagtc gatattgctc tgatactgct caatcgccga
    23881 ctgctggcgg tttaactggg tgattcgcgt ggcggcgatc gggtcatccg acggctgcaa
    23941 aatctgcttg ccggttgcca tctgctcgac aacatgcgcc agatcgccag agagattatt
    24001 aaaactttgc gtcatcgaga cgtaggtttg ttgggtagtt actcgcattt tcgcgctccc
    24061 gcttagctgc acatttccag caccgaatcg aaaatctcgg ccccggcgga aatgactttc
    24121 agattggctt cataaatttg ttgataggtg atcaggttca ccgcctcttc gtccatgctg
    24181 acgccgctga cgctgctctg ctggttttgc gctgcggaat agacattgga cgcggcatcg
    24241 acttccgtct ggttttgctg actgtaaatg ccgatattgc tgatgattga cgagcacgcc
    24301 tgcccgaccg tcacgctgcc gaggttggct atttccagcg gttcggtgga gatattgatc
    24361 agcgcctgca ggttgtcgct gttgccgctt tcgtccggtg aactggaaaa cgccagctca
    24421 tcggcggtga tattcgggtt aacggtcagc gggccatcgg cattgctggc gtcataaata
    24481 aacagcggct cgccggggtt accgttgaga tcgtagccct gcgccagctg gttgttgacc
    24541 gcatcggcga actgcgacgc catgctgttg atggtgtcgg tcagcggcgt cagcacatcg
    24601 ttttgataat caaacagtgc gcctaatgaa ccgccggtgt cggtggtcat cgtcgaggtg
    24661 gtgccagcga aggtcagcga catggtcgac gtgccatcgg cgttggtttc cagcgcgatg
    24721 gtcgaacttt gctgcccact caccagcggc tgaccgtttt tcagggtaac gttgtagttg
    24781 ccctggtcgt cgatattgac ctgcacatcc atcatcccgc tcagttcttc caccatctgg
    24841 tcgcgggcgt cgtacagcgc ggaggcgtta tcgccattgg cttcagcctg agcgatttgc
    24901 tggttatagc tggcgatgcc gctggtgagc gtattgattt gcgacaccat cgcctgctgc
    24961 tggctgatga tttccgtgct ttgcgagtcg atgtaatcca gcgtgttatc aatacgcaac
    25021 gacagcgccc cggcttcact gatcacctgc tcgcgcaggg cggaatcatc ggggctggtg
    25081 gttgcttcgt taagggcggc gaagaagtta tcgaagccgc cgctcaggct gctgttatcg
    25141 tcgctcagta ccgcttccag ttggctgaga tccccctgct gggtgctgta atagccgtaa
    25201 tcgctggcgg cataccacac ctgattcacc tgatactggt tggagacgcg gcgaatgctg
    25261 tcgacctgta cgccgttacc ggcgctgttc ggcgaaccgc cgctggcacc gatggtgctg
    25321 atctccgcca cctgacgggt gtagcccgtg gtcatggcgt tggcggtgtt ttgcgccgtg
    25381 acgttaagct ccacctgcgc ggttgatgca ccgctgtagc cgatgttaat catgtccatg
    25441 ttggatcctt accggtaaat cgtgccggtt aaaagcggcg taaagttggg gattcttaag
    25501 ttccgtaaac tgggtgccga acgccggatg cggcgtggac gccttatccg gcctacatac
    25561 gggcgactcg taggcctgat aagacgcgac agcgtcgcat cgtgcaccga acgccgaagc
    25621 tcatttatgc ttcggcgaca actgcttcac aatcatcgcc gcaatgccaa tcccgtgctg
    25681 ctgcgccagg gtgttacaca actcatcgtc ataaaacccc tgcatcatgc gcaccgaatc
    25741 gctgttaaac gggttatctt ttgaatcaaa caactcgttg gttttccgca tctctttcag
    25801 catgttgcgt aaaaaaatcg cctcaaactg ttcggcggcc tgcttgatat cattggcctg
    25861 aaccttcggc cccattaacg cgtcgctgcc gtcaatcccg ccgctggcgt tcactttcat
    25921 cagatcacct ccaggtcggc atccagcgcg cccgcctcat gcagcgcctg caaaatcgac
    25981 ataatgtcgt ccggcgtcgc gcctaaactg ttcagcgcat tgacgagcgt tttcaggctg
    26041 ctcgactccg gcaaactcac cacgcccgga cgggcatgat tcaccgcgat attgctctgc
    26101 ggcgtaaccg ccgtgcgccc acccgcaaag gcattcggct ggctgacgtt gctggtttcg
    26161 ttaatcgaaa cggtcaggct gccgtgcgac accgccgccg cgtgtagcgc aacgccatcg
    26221 cccattacca ccgtgccggt acgcgagtta aacaccacgc gagcgcggat tttctccgcc
    26281 tgcacctgca catcgtccag ttgcgacata aatgccaccc gcgcgccggg gctggtgggc
    26341 gcgcgcacgg taacgttggt ggaactctgc gcggtggcaa taccgccaaa cgaggcgttg
    26401 atcgccgccg caatgttgtt ggcgtcttta aacgacgggc gtttcaggtt gagggtgatg
    26461 gtatcgccca tctgaaaatc gctggggatc tcgcgctcca ccgttgcgcc gttaggaatt
    26521 aacccggcgg ttggcgtatt aaccgttacg ctggaaccgc tggccccggt ggcgttcatc
    26581 ccgcccacca ccacgctgcc ctgtgccagg gcgtaaactt ccccatcagc gccgtgcagt
    26641 tgggtcagca gcaaagtgcc gccgcgaata cttttggcat cgccaatcga cgaaacggtg
    26701 acatcaatgg tctgcccgcg cgagtacatc ggcggcagcg tggcgctcac cgccaccgcg
    26761 gcgacgtttt tcaccttcgg gtcgatttta ttcggcagtt gcacgccaaa ctggcgcagc
    26821 atattggtga tggtctggtt ggtgaatttg acctggtttt tatcaccggt gccgtcgagg
    26881 ccgaccacca ggctatagcc caccagttgg ttttcgcgca cgccctgtac gttgaccagc
    26941 gactccagcg attgcgccag cgccacacca ggcagcgcca ggctcacggc gataactgcg
    27001 gcctttatcc agttttgcat tgcactctcc ggcattaaat cgggaacaga ggatggttaa
    27061 acagccgcgt cagccagcct gcggcgttgg cgtcgcttaa cgcgccgcgt ccggcatagg
    27121 agatgcaggc atcggcgatc cgctgggagg aaaccgagtt gtcgttctgg atgtcgtcgg
    27181 cgcgcaccag gccgcttaag cggatgtact catcgccctg gttgagggtg agccatttct
    27241 cgccgcgaat ttccaggatg ccgttgggct gcaccgcatg gacggaaacg gtgatctccc
    27301 cgcgcaggct gttctgctgc tgggaagtgg cgctgccgtc aaagtcgcgg ttggcgtcga
    27361 tagagccgga aagcttatct ttagtgtgac taaaaatggt cggtgcgccg atatcgacgg
    27421 tgttgctttt gccgaagttg gttttcgcct gtttgctcga ctgggtggat tcttcgagga
    27481 tcaccgtcag gatatcgccc acgcgataag cccgccgatc ggcggtcagc gaccagttgt
    27541 agccagtttc aaatacaccg ccagcacgcc cgtctgccgg aggcggtgct tcggttttcg
    27601 gtggcgcaaa gtatgcgtcg tcttttttga ccagaatggc ctgcgattca cacccgctaa
    27661 gcaggggaag cagcgccacc agccagagat aatttttcac gttctgcctg tttaaataca
    27721 gtgagttatg cgcttttgta ggtcggataa gacgcgtcag cgtcgcatcc gacatttcgg
    27781 ccttacagcg tctgactgat gtactgcaac atatcgtccg cagccgacac cattttggcg
    27841 ttcatctcgt aagcccgttg cactgtaatc atcgccacca tttcgttgac gatatctacg
    27901 ttcgagcctt cgagggcatt gtcctgcaaa gtgccaagcc catcttcgcc cggcacacct
    27961 tcggtcggct gaccgctggc ggcggtttcc agatacaggt tgtcaccctc agcggaaagc
    28021 ccagccgggt tagcgaagtt aaccagcgtc agttgcccta actcggtcac gtcgctgtcg
    28081 ccaggaagaa tcgccgtcac cgtgccgtcc tcaccaaacg caacattcgt cgcccccgcc
    28141 ggaacgtcga tttccggctg caatggcaag ccctgtgagt tggtcagtac gccatcggcg
    28201 ttcacctgca aattgccgtc gcgggtatag gcgatatcgc cgttggcctt ttgcacctgc
    28261 aaaaatccct gccccataat ggcgacattc atcgcgttgt cggtggtttc gacgttgcct
    28321 tcggtgaagg ttttctgggt gccgacaatg cgcacgccgc taccgaattg cagcccggtc
    28381 ggcatgatgt tgttctggtc gagcatcgcg cccggcgctt cctgggtctg gtagaacaga
    28441 tcctgaaaca tcacccggtc acgcttgaag ccggtggtgt taacgttggc gatgttgttg
    28501 gcaatcgcgc tcatttcagc atcctgcgcc gagaggccgg ttttgctgat ccataatgct
    28561 gcgttcatgg tggtaaattc cgtgttattt ttgggccgcg gttgccggat gcggcgtgaa
    28621 cgccttatcc ggcctacgat gtgcggttac gagccgcgca ataagcggtt gcccgcgtcg
    28681 ctgatatctt cggcggtttt catcatcttg atctgtgctt cgaactggcg gttcatggcg
    28741 atggacgaca tcatttcgct taccgccgag acgttgctgc cctcgagaaa accgccgctg
    28801 actttgatgt tttcatcgcg ctgggccgga acgccgtcgg cagtcaccag catcccttca
    28861 gcatttttct ccaggttagc caccgggata tccaccagct tcaggcgatc gatatccatc
    28921 gtcgcggtca catcgccgtc atcgggcgtc accgacagcg tgccgtcgct gccaaacgaa
    28981 gcaatggcgt tcggcggcag aatgatcggc ccgttatccc ccagcaccag attgccgtcg
    29041 atggtcagtt gcccctggtc gtcctgctga atgttgccgt tgcgggtata gacttcgtta
    29101 ccgtttttat cctgcaccgc aatgtaaccc gcgccctgaa tcgccacgtc cagcgggcgt
    29161 tcggttttct ccgcaacacc cgtgctgtcg ttcacgccgc tggccccttc ctgcgccata
    29221 tagcgcgtgt cgaagccgcc gcctttcacc ttgtcgttgg ttgccattgc catatcggcg
    29281 cgaaaaccat tggtgttgac gttcgccagg ttgttggcgc taatttgctg ctcatacagg
    29341 gtctgagaag caccgctcag ggcggtataa atcaggcggt ccattacatt gcctggaaca
    29401 gcgcgtcatc gagctgcgtg ctggtggcga taactttggt gttggcctga tagttacgct
    29461 gggcggtcat caggttgacc agttcgctgg tgatatcgac gttagagctt tcgagcacgc
    29521 ccgacgacag cgtaccgcag gtgccggagc cgggaacgcc aatcagcggg gtgccggatt
    29581 cgcccgtttg tacccatgcg gtgccgctca ctgcctccag gccgttttca ttcgggaaag
    29641 tcgccagcac cacctgcccc tgcaacatgc gctcgccgtt gctgtaggtg gcgtaaactt
    29701 tgccatcgtc atcaacctga acaccgtttt gcgtggcgga ggcgtagccg ttggcggcgt
    29761 tggtggtgac cgaaaattca gagccgtatt gcgtacaggt ggagtaatcg acggttaagt
    29821 cgacgggcgc ggcggcgtcg gtttgaaatt caatggtctg cggcgtggtt ggagaggtca
    29881 gcttcccggt gttcggatcg aaggttaacg tcgtcgccgg aacgccggtc tgctgctgac
    29941 cgtcgaaggt gtactgcact tcccaggtgt tgtcgctggt tttggtgaag tactggcata
    30001 ccgaatgttc gttgcctaat gagtcataca ccgtggtggt gtagctgtcg gtgtacgaac
    30061 tgctgttagt cgcgtcgaac ggaacggtgg tgcgatcgat agcttcatcg ctggcgttga
    30121 agttagcggt aaaggtcagg ctgctgctgg cctgcgccgg gatattgccg gttttgatct
    30181 ggatatcggt gaccgtaccg gtttgcagag tgccagtgtc atcgaccgga tagccctgca
    30241 aataagcgcc cgaggcgttg acgatatagc cgtttttgtc ggtttcaaac gatccggcgc
    30301 gggtatagga gatattgcct gcgctgtcac acatgacaaa aaatccgtcg tcgttaatcg
    30361 ctaaatcgag cgcattgccg gtggagacca gcgaaccgcc acgcgaaata ctttgcgcgg
    30421 tgcccgccac gctgacgccc atcgcctggc ttccggcata catggcggaa aactgggtgg
    30481 tcatcgactt atagcccacc gttccggcgt tagcgatgtt gttactgatc ccgtccagct
    30541 gttcgttaac ggcattcagc cccgttgcgg caatttcata actcattgtg tcttcctgtt
    30601 aattgattct tacgccacat cactgtcggc atcgggtacg ccaaactggc taatcatggt
    30661 gaacggcact tcgcccacgc ctgcaacgtt aagcaccggg gaactgccgt cgagcgggat
    30721 gcgcacatct tccacttcgc cggagacttc aatcggcacc tcttcttccc cggtattggt
    30781 caccaccgag acgtcgtaat cgcccggcgg gatgccgtaa tccgcgggat tcaccgagaa
    30841 atcaacctgg cctggtccga cggacgaact gccttctggg atcagcgaga cggtgtagtc
    30901 atcgcctgcg gcatcggtaa agtggagatc ggcggtggta caggcgtcgt ccagcgtagc
    30961 gcgaccgtta atggtctggt cgctcacctg caaggcggtg gtctgcacca taatgtcgtc
    31021 gcccaccagg ttaccgagcg ccatcacctg aatgttgctc atcagcaccg cgttggtgtt
    31081 ggcctgcacg ctcatctctt ccatcatcgc cacctgcgcc atcgacgaca gctggtcgat
    31141 gtattcggta gcgtcggtcg ggtcctggtt ttggatctcg gcgacaaaca gcgtcaggaa
    31201 ggtatcgaca ggcgagctat cgctgctgct ttgcgtgtcg ctggccgcca cattattgct
    31261 ttccgccgtg gcggtcggct gcggcgattg cgcattcagc gccagggtat tcatcaggct
    31321 tctcccagtt tcagcaaact ttgctgcata cttttcacgt tgttgagcac atcgacgttg
    31381 gtttcgaaat cgcgcgaggc cgacatcatg tccgccatct gctccaccac gttgacgtcg
    31441 ggataccaga cgtcaccgtt ggcgtcggcg agcggagaat gcggttcata gcgtttcacc
    31501 gccccgccgg tttgcagcac gtcctgtacc tgcacgctgg caccgtcaat ggcatgacga
    31561 tgtgtacccg ccagcaggct gtggtgatac accgcggcaa acaccgggct gcgcgcttta
    31621 tacgcctgcg cctcgctgct ggcgggcgca ttggcattcg ccaggttact ggcaacggta
    31681 ttcagacgta gcgtttgcgc cgtcatcgcc gagccggaaa tctgatagat atcagtaaat
    31741 gacatgctta tttcccttca atggcctctc tgatgccgct gatttgcaga ttcagaaagg
    31801 tcaaactgct ttgataatcc atactatttt gtgaaaaccg cgcctgctcg ctgttcagtt
    31861 ccacggtgtt gccatcttcc gacggctgca tcggcacgcg gtactggaca tccaccgccg
    31921 cgttatttgc ccgttgcatc tccctggcaa aatcaatatc tttagccttg aaatttggcg
    31981 tatcgacgtt cgccagattc gcagtcagta gctcggtcct ctcaagacgt aacttcactg
    32041 cctgcggatg tactcccaat gcctgctgaa aactgatccc catttcctgc atcactcctg
    32101 cttcaatccg catacgctaa gcaatcgcca tgccattttt taagtcactg atgagaaagg
    32161 gtttatgagt tttcaatttt gcaggcggaa gttttacttc ctcctggcgc tggccttacc
    32221 gggctacgcc gccgtccatc ccgtgcaaca cagcgcgcgg gaacaagtca acgcccaggt
    32281 gctgaatgcc gcgagccaaa aaattgaatc gctggcgcag cagcggcagt ggcatgacta
    32341 tcgctacacc tttaagatct acattccctc gcagattgcg accgccgcgc cctgtactaa
    32401 aacacccggc gtcacgctga cttcgcctgc ggaaatcgcc ctcaaccgga tgaacttcac
    32461 cgttagttgc ccgcaaagct ggcagatgaa cgtggcggtg cgcccggatg tactggtgcc
    32521 ggtggtaatg acgaagtcac tggttgcccg cgatatgccg ctcaccgcga atgatgtgga
    32581 gcttaagccc tacaacgtca gcgcccagcg ccgcgatgtg ttgatggagc ttgatgacgc
    32641 gattggttta agcagtaagc acgcattgca accaggcaga cccatcacca aagaagaact
    32701 ggtgtctccg gtgctggtcg ggcgcgatca gccggtgatg attgtttacc agagcgcggg
    32761 gatcaccgcc tcgatgcccg gcgtggcgct gaaaaatggt cgtaagggcg agatggtgaa
    32821 agtgcgcaat gccagcagcc agcgggtcat cagcgcgatg gtggcggaaa gcggcgtggt
    32881 gacaacggta agcgcggagt aatttgcgca ggcgattaaa gttttgccgc ctgttaaccg
    32941 ctttaccgca gtaacccacc caaataccga gacgaggttg cgatgaaaat caccccgact
    33001 atgcctggca atcgcccgac agccacaacg gcaggctcca gccagccgcg caaaaccacg
    33061 gtgaccacca ccacgacgct gagcgccgac gacattactc aggcgggatt gcagtctgcc
    33121 cagcaaacgc tgaacgacca gcaagagagc gatatcgaca gcaacaaagt ggcgcaagtg
    33181 caggcgatgc tggcgtccgg cagcccgcaa gtggacactg aacagttagc cagcgacatg
    33241 ctgagttttt tccagaacta agaggccgat gaagtgagta tccggttaca acaggtgaag
    33301 gcgttgttgc agggcattcg tgacgatgac gcgctgtatg attccctgcg cgaacttctc
    33361 cagcgtcagc ggatctgcat gattcgccgc gccagcgaag agttgctggc ggtaaacgat
    33421 gagataaccc atcactatga gcagttgcat ggacatagcc atcagcggca tagcctgctg
    33481 aaaatgctcg gtgttagcgt aaatcgtgac ggtctggcgc aggttttcac ctggcttccg
    33541 gctgttcaaa aagcggctgc acaacagttg tggcaacgcc ttgagcaaaa agcggaacgc
    33601 tgcaaagcct acaacgacaa aaacggcgag ctgttgattc gccagtatga atttattcag
    33661 tcgtttttag gcagcgaagc agattttctc tatcaggaat aacgcgaata ccttccccat
    33721 agcggaaatg gattttccgc ctgatttcca atcccttgtt tttaattaca aaaatccccg
    33781 gcacgcttat tgcgtatcct gcacagcatt aaagactgga agccacctat gagttttatt
    33841 gaatgttacg aaccggaata cgtgcgcaaa tttttagcgc gtcaccccgg ttcgccttta
    33901 tacgtccgca aggtgtggaa aaacgagatt cgcctgagcc tgtcattgac cgatattgcc
    33961 agctgcgaag cggtgctcaa cgatgtcagg gcctttgatt tacagctcac ctaccgccct
    34021 gtggaagata attccgcaga gctttccgcg cgcgatgcga tagtaaacca ggtgattttg
    34081 agtaccctca cgctccgtga tttaaccccg gagctttccc tgtatgctgt cgggattttg
    34141 ctctcctgcg ccagcaaaat gccgggcaga gacggagaca tcctggcccg tttgacgacg
    34201 ctgccacagg cgctggccga tcatgcgaaa aaaggtacgc tacaggcaca gtacgcccag
    34261 ctcccgcccg tgccacagct ggcgcgccag ttgatgacgc tgctcggcgg ttgtgcgttt
    34321 gactggtcga ttttgccgga aagcccgcgc aaagcctcgc tgccgttgca ggttacttta
    34381 ctgacgctgc atgacgccaa cagcgaagcc ctgttacaac agcagcttca gacgcagtgg
    34441 caaaccacct ggcaacagca ttttgccacc gcgccctgga tgatgcgtaa ctggctgatt
    34501 taccgcgttt accacgatat gattgggcag actgacgggg cggattattt cccgctggtg
    34561 tgcgattttt atctcctccg cacgttaatc agtttatgga cgctggatgg ttctccgctg
    34621 cggcaggagg atattttcgc cctctttgcc atgtttgaac gctggcgagc cagtgaaaat
    34681 gcgctgttgg ttcgccagca aattcagtcg ttatgtgccg ccgacccgct gctttcagcg
    34741 ttttcgctgc taacgtgagt cgctctgcgc gcgctttgcc cctcacccta accctctccc
    34801 caaaggggcg aggggaccga tcgagcacgg tgttaaaatc ggcgctgatt ttctccctct
    34861 ccctgtggga gagggtcggg gtgagggcat cagcgcgcac gttcatcaca ttcacacatt
    34921 aactcaccca aacaccatgc caacaccaac catcatcaaa acactcgcca cccaccgcga
    34981 aaaaccgttt gtcagcgtcg tcacgccaac ctggaaccgc ggcgcattcc tgccgtacct
    35041 gctctatatg taccgctatc aggattaccc ggcagaccgc cgcgaactca tcatcctcga
    35101 cgattcccca caaagccatc aacacatcat cgaccgcctg accaatggca cgccggaagc
    35161 ctttaacatc cgctacatcc atcacccgga aaaactgccg ctgggcaaaa aacgcaacat
    35221 gctcaacgaa ctggcgcgcg gcgaatacat cctctgcatg gatgatgacg actactatcc
    35281 ggcagataaa atctcttaca ccatcgctat gatgcaaaaa caccgggcgc tcatctccgg
    35341 ttccgaccag atccccatct ggtacagcca tatcaaccgt atctttcaaa gccgcagctt
    35401 tggcccacat aatatcctca acggcacctt ttgctatcac cgtaactacc tgaaaaagca
    35461 ccgttacgac gatgattgca atttaggcga agagaaaagt ttcaccgaca atttcagcgt
    35521 taacccgctg caactgcccg gcgagcgcac gattttgtgt atctcgcata gccacaacac
    35581 ctttgataaa gactttatta tcggggccag cacgccggta aacacggcgc ttacggatat
    35641 cgtccgcgat ccgctgctgc gcaatgctta tctgagtctg cataacgcca ctcatcacca
    35701 ggcgatcaac catcaggcga tcgatcaggt ggtcgtcgtg aatctggata aacgcccgga
    35761 tcgcttacaa cagatccgtg aagaactggc attgctgcat attccacccg agaagatcac
    35821 ccgccttgcc gccagtgaag atgaaaacgg tcaacggggc cgccgtcagt cgcacttgca
    35881 ggcgctgcgt ctggcgcaac agcacggctg gcaaaactat ctgttactgg aagatgacgc
    35941 ggttattctc aagcaggaga aacatattca ggtactgaac acgctgctgg cgagcctggc
    36001 gaaaattccc tggcaggtga tgatcctcgg cggcgaaatt tcgcaaggaa cgatgctaaa
    36061 aagtttgcct gggctggtgc acgcgcggga ctgccgcaaa gtgtgcgctt atctggtgaa
    36121 cagccgctac tacccgcaac tggcgcagca gatgagcaac gatgagcact cgctggaaga
    36181 ctgctggcaa ccgctgctac gcgccgacaa atggctggcc tgttacccga gcctttgtta
    36241 tcagcgaccg ggctttagcg atatccagaa aaaaacgact gataatattg ctcgctattt
    36301 caataagtta cccgtaacca ccaagccatc cgcgttaccc atcgccgaca ccatcggctt
    36361 ttttatggaa acctcattcc actacgcgct gtaccgccca atcatcaccg ccctacaggc
    36421 gcaagggctg agctgcacgc tggtgatcaa cgaccgcgtg tttaaaccgt ttctggatga
    36481 gatgctggaa acactgaaaa acatcgacga tccgcagctt aaaggtatgc gtctgtcaga
    36541 aatgcagaca catggtcagc gggtgaaatg cctggtcagc ccctaccaca cgcctgcgct
    36601 caatggcctg gcggcggtga acatccgcgc tatgtacggc ttagccaaag agacgtggaa
    36661 ccacgccgac tggaatcgtt tttaccaaag cattttgtgt tatagccact acagtcagca
    36721 ggcgcttgcg catttcggca gcgcaaaagt ggtcggcaac ccgcgcttcg acgcctggca
    36781 taacggcacg ttcgatcgcg cgttgccgga aaacatccaa tcagattacc gtaaacctac
    36841 ggtgctctat gccccaacgt ttggcgcatt aagctccctg ccccactggg ctgaaaaatt
    36901 gggccgcttg agcggtgatg tgaacctgat ttgcaaactg caccatggca cctgctcacg
    36961 cccggaagaa gcagcatcgc tggccctggt gcgcaggcat ttaaaacagc gcaccgactc
    37021 cgcccgccac acgctggcgc tgctggcaaa agccgattac gtgctgaccg ataacagcgg
    37081 gtttattttc gatgctatcc acgttgataa gcgggtgatc ctgctcgact ttccgggcat
    37141 gaatgatttg ctcgacggtg agaaaagtta ttccacggca gagagcgccg accagcgaat
    37201 ccgcgaaata ttgccggttg ctcatgatgt tgcagaactg cgttatttgt tgtcagaggc
    37261 gtttgactgg gggtcagtgc aggcgcggct tacggagatt cgccatcact attgcgatgc
    37321 gtttatggat ggcaaggcgg gagaacgggc ggcgattgtg attgtggagg cgctggggta
    37381 aaccgcgcaa ctgcctgatg cgcttctctt atcaggccta cggatcgatc acgtcctgta
    37441 ggccggataa ggcgtttacg ccgcatccgg caagaaaata catctctgtt ttagtacttt
    37501 ataaaagccc tctaatcacc tcaataatcc ccgtcgtcga caccgacggc gtgcggggga
    37561 aatagaccac ctcacaaata tacgcaaagc tgtcaaactt ccctgcccag tcatccccca
    37621 tcactaaaat atccgcactg tacccgcgca aatactccgc tttctgctct agcgattctt
    37681 ccagaaatac gccatccacg cacgccagcc cggcgatgat cgccatgcgg tcatcctgat
    37741 ggtaaaccgg tgccctgccc tttttggcga tgttcagggc gtcggacgat acgccgacca
    37801 gcagccgctc gcccaatgcg cgggcgcgtt gtaaaaggcg cagatgaccg acatgaaaca
    37861 catcgaaggt gccgaaggtg atgaccgttt tcatggctac tccgctaatt tgtgccgcag
    37921 ccagcactgc acggcgatgt cgtccaccgc tttttgcgct ttcgccgtgg cgctttcttg
    37981 ttgctcgcgg cgtttgtcgt ccagcgtcag ggcgactact ttctcttcgc acgccgccgc
    38041 cgtgaggttt ttctgcattc gcgtggcttt gatattggcc agcgtttttt cctgctcctg
    38101 ccaggcgatt accttgcgca gggtgccttt gtagccgctg acgtttttca gcgattccac
    38161 cgacaccgcc gcacccgcag atgttttctc gacaaggtag cctaacgcct tgatattgtt
    38221 gtcataaccc gcgcaaatct gtttttgctt tgccagctcg acggttttgt ctttgaccga
    38281 tttatcccgc aggcgctgca actgcgtcag ggtgtcgatg atttgtcgca tagttactgt
    38341 tctgcctttt tgacaccagg aaagagaatt tgcaggcggc tttgcaccag ttcgagggag
    38401 gcgggttcgc gcatctcctg gcgcaaaaat cgctcgatgg ccgggaacat ttttaccgct
    38461 ttgtcgacgc tggcgtcggc acctgccaca taaccgccga gcgggatcag aggtttgatc
    38521 tccatatagg cggcatagtt ttgctttaac gcgcgggcgg attgctgatg ttccaggctg
    38581 gtgacctggt tcatgcagcg gctgatcgac tggccgatat caatggccgg atagtgcccc
    38641 gcttccgcca gtttgcgggt taatacgata tgcccgtcca gcaccgcgcg cgcgcagtcg
    38701 acaatcggat cttgctggtc gtcaccttcc gccagcacgg tgtagatggc ggtcatcgat
    38761 cccgcgcttt cgctgttgcc tgcgctttcg accagtttgg ggatcatgcc gaatgccgat
    38821 ggcggataac ctttggtggc gggcggttcg ccgagcgaga gggcgatttc ccgctgcgcc
    38881 atcgcgtagc gggtcaggga atccaccagc agtaagacgt gatgcccgcg gtcgcgaaac
    38941 caggcggcga tggagtggca cagttcggtg gctttcagac gcatcagcgg cgattcatcg
    39001 gcaggcgcga cgacgacgat agactttgcc agcccgtccg cgcccagcga gtggtcgata
    39061 aactctttca cttcgcgccc gcgttcccca atcagcccaa ccaccacgat atcggctttg
    39121 gtctggcggg tgatcatccc cagcagcacg cttttcccga cgccgctgcc cgccattagt
    39181 ccgacgcgct ggcctttgcc gatggtcagc aggccgttga tcgctttcac gcccacgtcc
    39241 agcggttcgt ctaccgaacg acgagttaag ggattaaccg agggcggcaa cggcggcaga
    39301 aggtcgttgc cgttcaggcg gcctttgcca tccagcggtt cgccgaggcc attcaccacg
    39361 cggcctaacc agctttcgcc tatcaggatg tcgtgggctt tttcttccgg gaagacgcgt
    39421 gcgcctgcca tcaggccgac cggctgttta aacggcatta aataggtgat gtcgcgatta
    39481 aagcccacgg cctgcgcgtc gatcagcgtg tgattggcac tttcgatgcg gcacagctgc
    39541 ccggtcatta gcgggcagcc gacactctcc agcaggatgc cgttaaggcg caccagccgc
    39601 ccggcgacgc gggccagcgg gatcgactcc agcgagcgca gggcgttatc gaacgcagaa
    39661 aaatcactca ccctgcgact ccggcgtgag tgtttctttc agcgcggtca tgcattgttc
    39721 aaggcgatgc tcgcagccga tatccagttc ggatttgtcg gtgatcaccc ggcattcgcc
    39781 cggtggcaaa tccggcgaag gcgtcagccc ccattcgctg accttttccg gcaccgcgtc
    39841 gcgcaggcgg ttaaactctt ccgtgctcag cattacttgc agggtttccg gcatcgccgg
    39901 gaaggccgcc agcgcctctt ccaccagcgc cagcaactgc gtgggttgca gcgccagttc
    39961 acaacggatc acctggcggg tgactttttc caccagttgc agcaaatctt cccgctgttt
    40021 acgttcgata tgagcgagga agtcgttcac tttaccggtg atggcttcca gcggctgcgc
    40081 cgcctcaaca aactgtttgc gcccttcctg ctgcccggcg aggctgcctt cggtgtaacc
    40141 ctggcgtcgc ccctcggcaa aaccttgctg atgaccttcg ctaaagccct gctcctggcc
    40201 ttcggtcatt ccttgagcaa accctttttg cagcccttcc tgaaatccgt ccatcaactg
    40261 gcgctggtaa tcggcggcgc taatactggt agtgagcggt tctccggtca ccgcacgctg
    40321 gcgcggcggg aaacggtgca ggcgatagcg cccgcgcagt gtttcaatgg tcatggttac
    40381 tccgccgtct gttccgcaaa gagctgtagc tgaatttcgc ccgcctcttc cagttcacgg
    40441 gcgatgccca tgatttcgcg gcggatctgt tcgatacggc tgaccggcac cggccccaga
    40501 cgcgcggtga tggcttccag ttgctgcgcc tgccgttttg gcattaccgc gtaaatcgaa
    40561 cggcgcagca gggcttcggt gcctttcagc gccaccgccc agtcttccat cggcacttcg
    40621 tcaagcagac gtcgacgcac ctcttcgttc tggcggctga ggataaagaa gtcgtacatc
    40681 tcatcctgaa gctgctccag cacctcttcg tcgcgctcgc gcatctggtc gaggatcacc
    40741 tgctggttgc cctggaagcg gttgacgata tccgccgcct gtttgatgcc tttcactttt
    40801 gagccgtgtt ccgaaagcac cgacaggccg cgttcgatca gcctgtccag ttcatccacc
    40861 acgtcgcggt tgacgtcgtt gagtttcgcc acccggtaga gaatttcgtt ttgcaccgat
    40921 tcgttgagat acgagagcac cgctgccgag atttccggcg tcagaaacgc gaggaacacc
    40981 gcctgtaatt gcagatgttc ttcggagatc agcatcgcca gctggcgtgg ttccacccat
    41041 tgcaggcgcg ccatccgcga gcggatctca tcgccgtaga tgccgttgat cacactgctg
    41101 gcgatctcgg tgccgagggc tttgttgaga ataccctgca acatggatcg cgatgcgccg
    41161 ttgatcccgc tctgttcgcg gaactcatca aagaagttat tgatcacttt gcgcgccatg
    41221 ctggttttca cgccggaaag ccgtgccatg ttttcgctca ggcgccccac ttcttcgcgg
    41281 ctgagttttt gcatcaccgt cgccgccgcc tcttcgccca gacacagtag caggatcgcc
    41341 gcctgttcca ggtagctgtt gctcttattt gttgtcaatt cgctcattgc tggtgatcca
    41401 ttgtctgaga acttcggcta cgcgatcggt gtcgctcatg gccagttttt gcaggaactc
    41461 gagttttact tccaggccgg aactctgaga cggcaggctg tcatcgccgg gcagcgacgg
    41521 cagatcgacg tttttgcgct cctcttccgg catggcaatc cacggttcgc tgacggcttc
    41581 cgattctggt gtgataatcg gtgcacgtac cgccgtcagc cgtttcatta ccgggcgcac
    41641 cacgaagaac agcaacaaca gcgccagcag gccgcagcca atcaggcgca cccaggccag
    41701 cacgctgtca tctttccaca gcggaataat tggttcgacc ggcaccgcct gcggcacaaa
    41761 gttaagcagt gaaagcgtca ggttatcccc acgttgcgcg tcaataccgg cggcattgtt
    41821 cagcaacgcg gtaagctgcg cggtctgctc cggcttccag tttttcagcg ccggagcgtt
    41881 ttgattgagc accaccgcca cattgaggcg tttcaccgca aagccgggat gctgaatatg
    41941 ctggacgcta cggtcgtaag agtaatcgcg cttgctttcg ctgtgtttgg ataacgcttc
    42001 aggcgagcga ttttcttccg taccgttagt catttgattc gccgcaaccg gcggacggtt
    42061 gctgagagag ccgggcacac ccatcgccac ctgattggtg tcgctgtcca gcacgctctc
    42121 ttcgcggttt attttcggcg cgtcgccgta gtgttcctga gtttcgtcga tggtgctgag
    42181 gtcgagatcc ggcatcacgc tgacacggta attcccgctg ccgaccagcg aatccagcac
    42241 gttcgccacg ctggcgtggg ttttgtcctg aatgtctttg aggatctgat cgcgtttacg
    42301 ggtagcggca gagaccgcct cgcccgcgcc gatgccgtcg gtcagcagat ttcccgcctg
    42361 atcaaccacg ctaactttgc tggcgtgcag ccccggaatg ctgccggaaa ccagatgcac
    42421 aatggcgttc acctggtcca tattcagctt cgcgccgtag tgcagacgca ccaccaccga
    42481 ggcgctgttt tgcggctcat cgctgaccac aaacgagctt tcttcgttga gcgccagatg
    42541 cacccgcgcg ctctccaccg cgtccagcga cataatgctc tgcgccagct ccccttcgag
    42601 gctgcgttta tagcgcacgt tctgcacgaa ctggctgctg ccaagcactt cgtctttgtc
    42661 catcagctcg tagccgctgg gcaaaatcgc ctgcacgcct ttggcggcaa gggtcatgcg
    42721 cgttttcgac agcgcctctt ccggcaccag tatctgcccg ctttgcgggt caatgcggta
    42781 ggtgagcttt tcgccgtcca gcacggtgac aatctgcgac acgggcagat tttcctggcg
    42841 tccgtagagc gagacgtagc cgcgattgcc ggtccacagc acgctaacga taatcgccgt
    42901 cgccgccacg gcaagcccca cacccgccat cagcgcccag cgtttgttat ctgcgagacg
    42961 cagtcgaaac gccggaaacg ccagggttaa cttctttatt tgtgcattca tagggttaga
    43021 tcgacatact catcagatcg ttaaatccgg tggcgatttt gttgcgcacc tgcaccagcg
    43081 cggagaaaga gagcgacgcc tgctggctgg cgatcatcgc acctgccaga tcatcgcttt
    43141 tgcccatgtc gatagccgtt tgctgctgct ccgccacctg ctggaactgg tcaacgtgac
    43201 cgagcgcgcc ctgcataatg cggtgaaacg aaacgtccgg gtcggtggaa ctgaaggtca
    43261 tggcgggcag caccggcgcc tgggcgttgg cctgcatccg ctgcacgtcc tgcatgatct
    43321 gcgcctgcat ggtgctgttg attgtggtaa tgctcatttt taacttccgg gtcaggagaa
    43381 aacttcgata ccgtgcttgc gcatggaggc cagtcgatag cgcagtgcgc gcggggtaat
    43441 acctaacagg tcggcgattt tgctgcggtt gccctgatat ttacgcatca ggtcagcgat
    43501 atattgatac tgggcgcttc gtccgtgctg gcctaaatcg ctggtttcgg caatgtgtac
    43561 ggcgggctga gcaggccatt gcagttcgct gtggtcggca atgtcatttt ccggcaggcc
    43621 taaggcgtcc gggtaaatca cgccgtcgcg atttaaaatc attccccgct gaatggcgtt
    43681 ctccagctgg cgcacattgc cgggccagcg gtaattcagc atcgcacggc gggtagattc
    43741 tgaaagggtg atatttttca ccagcaccgt ggagtatttt tttataaatg actctgccag
    43801 cggaataata tcgttcagac gttcacgcag cggcggcatg gtaataggaa taaccgccag
    43861 acgataatag agatcttcac gaaaacgccc ggcggcgact tcctgctcaa ggtttttatt
    43921 ggtgcaggca atcaggcgaa aattgagttt aatttgtcgg ttgctgccta atcgctcaac
    43981 ctgttgttcc tgcaatacgc gtaatatttt ggcctgtaac gccagcggca tatcgccaat
    44041 ttcatcgagt aataaggtgc cgttattcgc cagttccatt tttcctggca cactggcaat
    44101 tgcgccggta aatgcgcctt tgtcgtagcc aaataaggtt gcttccagca tgttttccgg
    44161 gatcgcagcg cagttcacgc cgatataggg cgcgttttca ttttcaccaa aggcgactgt
    44221 gtgaatatat ttcgccacgc actctttgcc cgcgccggtt tcgccctgaa tgagcaccgg
    44281 cacgttaaat gcggcgacac gctttgccag agtaaaagcg ttaatgctgg aagcggcagt
    44341 tgcaataagt tcagacatag ggtgaacgat ataagtagcg acaataatca ggatgttctg
    44401 aagcgtatca ccggcgggat taacaagttc gaattaaacg ataataaaaa cattatttgt
    44461 ttataaacaa tgatttatcc atgtaggccg gataaggcgt ttacgccgca tccggcaatt
    44521 gtgcaccggt gcctgatgcg atgcggcgcg tcttatcagg cctacggttc gtgcaatgtg
    44581 ttatgacgat gcctgatatc tcgcccttta ataatttaat tattttttca ccctattaat
    44641 aatttgcccc tttcacgccc cctctattta atacctcacc ttgtgtcact gccggatttg
    44701 cgtgcacaaa atcgcccata ccattacagc agagaaagta agccatgctg aagtacagta
    44761 aaacgccagg catctttaaa ttagaaggta atcggctcgg acgcccctac catcgcctgc
    44821 cgaccctgtt tacagggaat tttgacgtga ttgattcgca tctgggcagc tactttctca
    44881 aaaagcatcg cagcaatatc accctaaaaa agattggctg cgaaatggat attattaata
    44941 aaaacgctga actgatggtt tcccaggtcg ggcatctggc gtttgatatt gatcgctctt
    45001 tgctgttaat gctgctgggc aatttttatg gtctggagtc ctctcttgaa gaggcgaaag
    45061 cgcataacgg gctgccgacc aaaaccgaaa cgcggttaaa aaaccgtctg gcgctggata
    45121 tctgcacgct gatttttaat ctgcaaacct ccggtattgc gctgaaatta aagctggaca
    45181 gcagcacggt aattacccac tgggcgtatc aattaacctt tacccttgcg ggcgatgaag
    45241 agagctgttt tcgtattttg ctcgacgacg cccacaccga ttttatttta aatctgatcc
    45301 gccacagcga gcacagtaaa ccgcagcagg tggcgaaatc ggtcaataaa ccggccctga
    45361 ttaaagagat catccgctcc ctgccgctga ccctgaacgt caaaatcgcc gagctatcga
    45421 tgaatgtcgc ggatctcacg cagataaaag ccggggacat tttacccatc tcgctgggag
    45481 aaacgttccc ggtggcgatt gggcaatctg aattatttag cgccctgatt gtggaagaca
    45541 aagacaaact gtttttgtcg gagctggcgg gcaaaaatga gaatagccat gagtaagcaa
    45601 gaagatattt tagccgggga ttttggcctc accgatgaag ttgcgcctgt cgcaaaatcc
    45661 gccgacgctg aaacgctggt cacccgcctg gaagaccgtt tttccgactc gatgacgctg
    45721 ctaaaacgca ttccggtgac cttaacgctg gaagtgtcat cagtagaaat aatgttagcc
    45781 gacctcctta acatcgacga cgacacggtg attgagctgg ataaactcgc cggggagccg
    45841 ctggatatca aagtgaacaa tatcctgctg ggcaaagcgg aagtggtggt ggtcaacgag
    45901 aagtacggcc tgcgcgtgct ggaatttaac acccgcgata tcaacgacct ggcaccatga
    45961 aacgccgcac tcaactgacg ctgggccttg ggctgctggc gcttgcgccg gtggcgttgg
    46021 cccagggcgg cgatattgcg ctgcttaacg tggtcaccca cggcaacact caggagtaca
    46081 gcgtcaaaat ccaggtgctg attctgatga ccctcgtcgg tttgctgcct acgatggtgc
    46141 tgatgatgac ctgctttacg cgctttatta tcgtgctgtc gctgctgcgc caggcgctag
    46201 ggctacaaca aacgccgcca aaccgcattc tgattggcat tgcgctctcc ctcaccatgc
    46261 tggtgatgcg ccccatctgg ctcaatattt acgaccacgc agtagtgccg tttgaaaatg
    46321 atcagattac cctgaccgat gcgttaagca ccgccgcgac gccactcaaa cgctttatgc
    46381 tggcgcagac tgacaaaaaa gcgatggcgc aaatcatgac tatcggcggc gcgaagggcc
    46441 atgccgccga ccaggatctc tcgattgtgg tgcccgccta tgtgttgagc gagctgaaaa
    46501 ccgccttcca gattggcttt atgatttaca tcccgtttct ggttatcgac ctgattgtcg
    46561 ccagcgtact gatggcgatg gggatgatga tgctgtcgcc gctgatcgtt tcgctaccct
    46621 ttaagctgat gctgtttgtg ctgatcgacg gctggtcgct gaccatcggt acactcacca
    46681 ccagcattcg cgggctgggg ctgggctgat gatgctgacc atagacgtag ccgccgacat
    46741 tgtcgccagc ggcataaaag tggtgatttt gctggtttcg gtgctggtag tgccgagcct
    46801 gctggtcggc ctgctggtga gcgtgttcca ggcggtaacg cagattaacg aacagaccct
    46861 aagctttttg ccgaggctga ttgtcacgct ggtggtgctg ggcgtgtgcg gtaaatggat
    46921 gattatccag ttgcatgatc tctgcattca cctgtttacc caggccgcgt tgctggttca
    46981 ttgagatgcg cacaagcgac gttacgcaac tgatggatct ggcgctgggg ctgtggtttc
    47041 cctttgtgcg cattatggcg tttctgcgct atgtgcctgt gctggataac agcgccctga
    47101 ccgtgcgcgt gcggattatt ttgtcgctgg cgctggcaat catcatcacg ccgttgatcc
    47161 cccatcctgt tccccacgac ctgatatcgc tcaacagcct gatcctgacg gtggagcaaa
    47221 ttttatgggg catgttattc gggctgatgt ttcagttttt gtttctggcg ctacagctgg
    47281 ccgggcaaat tctctcgttc aatatgggga tgagcatggc ggtgatgaac gacccgagca
    47341 gtggcgcatc gaccaccgtg ctggcggagc tcatcaacgt ctacgcgatc ctgctgtttt
    47401 ttgccatgga cgggcatctg ctgctggtga gtgtgctgta taagggcttt acctactggc
    47461 cgattggcaa cgcgctgcat ccgcaaaccc tgcgcaccat cgcgctggcc tttagctggg
    47521 tgctggcgtc cgcatcgctg ctggcgctgc caaccacttt tatcatgctg attgtgcagg
    47581 gctgctttgg cctgctcaac cgcattgcgc cgccgctgaa ccttttttca ctcggttttc
    47641 ccatcaacat gctggcgggg ctggtctgct ttgccaccct gctctacaac ctgccggatc
    47701 actatctgca tctggcgaat tttgtgttgc agcaactcga cgcgctgaaa ggtcactatg
    47761 gcggatagca gcagcgaaga aaaaacagag aaaccctcgg cgcaaaagct gcgtaaagcg
    47821 cgcgaagagg ggcaactgcc gcgctcgaag gatatggggc tggcggcgag cctgtttgcg
    47881 gcatttgtgg tgatctccag cagcttcccg tggtatgccg attttgtgcg cgagagtttt
    47941 atcagcgtgc accagtatgc acaggagatt aataacccag aggtgatcgg ccagtttctg
    48001 cgccatcacc tgttaattct cggcaagttt attctcacct tgctgccgat gcccgccgcc
    48061 gcgctactct cctcactagt gcccggcggc tggctgtttc tgcctaaaaa aatcctcccg
    48121 gatttcagca aaatcagccc gttaaaaggc attgagcggc tgttttccag tgaacatctg
    48181 gcggaaacgg gaaagatgac ggtgaagtcg gtggtggtgc tggtgatgct gtgggtaagc
    48241 ctgcgcaata actttgcctc ctttctcggc ctgcaggcgc taccgttcaa actggcgatg
    48301 aatgagggtt tatcgctcta cgcctgcgtg atgcgcaact ttgtcatcct gtttatcttc
    48361 tttgcgttaa tcgacgtgcc gctggcgaaa gccctgttta ccaaagggct gaagatgacc
    48421 aaacaggagc tgaaagaaga gtataaaaac caggaaggca agccggaagt gaaggcgcgc
    48481 gtgcggcgtc tgcaacggca gttggcgatg gggcaaattc gcaaagtggt gccgaaagcc
    48541 aacgtggtga tcaccaaccc gacccactac gccgtggcgt tgcagtatga ctcgtcgcgc
    48601 gccgccgcgc cgtttgtggt ggcaaaaggc accgatgaaa tcgccctcta tattcgccag
    48661 gtcgccgctg agaatcaggt ggaagtggtc gagttcccgc gactggcgcg ctcggtttac
    48721 tacaccaccc aggtgaacca gcaaattccg tttcagcttt accgggccat cgcccacgtt
    48781 ctgacctacg tcctgcaaat gaaacactgg cgcgagggtg cacagccgcg cccggccctg
    48841 aacagacata tttccattcc caaagaggtg cttaaactgg atggcgaaaa caactaaatc
    48901 gttcctcgcg ctgctgcgcg gcggcaatct tggcgtgccg ctggtgatcc tgtgtattct
    48961 ggcgatggtt attttgccgc tgccgcctgc gctgctggat attctgttca ccttcaacat
    49021 tgtgctggcg gtgatggtgc tgctggtggc ggtgtcggcg aaacgaccgc tggaattcag
    49081 cctgttcccg accattttgc tgatcaccac cttaatgcgc ctgacgctca acgtcgcctc
    49141 tacgcgcgtg gtgctgctgc acgggcatct cggcgcggac gcggcgggta aggtgattga
    49201 gtcgtttggt caggtggtga tcggcggcaa ctttgttgtc ggctttgtgg tgtttatcat
    49261 cctgatgatc atcaacttta ttgtcgtcac caaaggggcc gagcgtattt ccgaggtttc
    49321 agcccgcttt accctggatg cgatgcccgg taagcagatg gcgattgacg ccgatcttaa
    49381 tgccgggttg atcaatcagg cgcaggcaca aacccgacgt aaagatgttg ccagcgaggc
    49441 cgatttctac ggcgcgatgg acggggcatc gaaatttgtg cgcggggatg ctatcgccgg
    49501 gatgatgatt ctggcgatca acctgatcgg cggcgtctgt atcgggatct tcaaatacaa
    49561 cctgagcgcc gacgccgcct tccagcagta tgtgctgatg accatcggcg acgggctggt
    49621 ggcgcaaatc ccttccctgc tactctccac cgcggcggcg attatcgtca cccgcgtcag
    49681 cgacaacggc gatatcgccc atgacgtgcg tcaccagttg ctggcaagcc catcggtgct
    49741 ctacaccgct accggaatta tgttcgtact ggcggtagtg cctggaatgc cacatctgcc
    49801 gtttttgctg ttcagcgccc tgcttggctt taccggctgg cggatgagca aacgcccgca
    49861 ggcggcggaa gcggaagaga aaagtctcga aacgctgacc cgcaccatca ccgaaaccag
    49921 cgagcaacag gtcagttggg aaaccattcc gctgatcgag cccatcagct taagcctcgg
    49981 ctacaagctg gtggcgctgg tggacaaagc ccagggcaac ccgctcaccc agcgtattcg
    50041 cggcgtgcga caggtgatat ccgacggcaa cggcgtgctg ctgccggaga tccgcattcg
    50101 ggaaaacttc cgccttaagc ccagccagta cgctattttc atcaacggca ttaaggctga
    50161 tgaagcggat attccggcgg ataaactgat ggcgctgccc tccagcgaaa cctacggcga
    50221 gatagacggc gtgctgggga acgacccggc gtacgggatg ccggtgacct ggatccagcc
    50281 tgcgcagaaa gcgaaggcgc tgaatatggg gtatcaggtg atcgacagcg ccagcgtgat
    50341 tgctacccat gtaaataaga ttgtgcgcag ctatattcct gatttattta actatgacga
    50401 tattacgcag ttgcataacc gtttggcgtc gatggcaccg cgcctggcgg aggatttaag
    50461 cgcggtgctc aattacagcc agttgctgaa agtgtaccgg gcgctgctga ccgaaggcgt
    50521 ttccctgcgc gatatcgtca ccatcgccac cgtgctggtc gccagtagcg cggtgaccaa
    50581 agatcatatt ctgctggcag ccgatgtgcg cctggcgctg cggcgcagca ttacccatcc
    50641 gttcgttcgc aagcaggagc tgacggtgta taccctgaat aatgagctgg aaaatctgct
    50701 gactaaccta gtgaatcagg cgcaacaggg cggtaaagtg atgctcgaca gcgtgccagt
    50761 ggacccgaat atgctcaacc agttccagag tacgatgcca caggtgaaag agcagatgaa
    50821 agcggcgggg aaagacccgg tgctgctggt gccaccgcag ctgcgccctt tgctggcgcg
    50881 ttatgcaagg ttgtttgcgc cggggctgca tgtgctgtcg tataacgaag tgccggatga
    50941 gctggagttg aagattatgg gggcgttgag ttgatggggg attcttttcc atgctttgca
    51001 gcacctgaca ccattaattg acgtcacact gcaaagagac tacgatgaca ccatacaatg
    51061 acgtcatgga ttgatagtat gattaaggtc agcgcgctag gttctcgtca tagaaaaaca
    51121 ttcgacgatg taatgacgga gccgccaaaa tctggaatta aatgggatga tgttgttgca
    51181 ctcattaaag ctgtgggtgg aacaataaaa aataacaacg gctccagacg aaaatttcag
    51241 attggaacga ccaaattcag cacacatgag ccacatccaa aaaatgtgat ggataaaggc
    51301 gcagtagcag gtcttaagga gtggtttgtt aactgtgtag gggtagatta tgaataaagc
    51361 atcgaacatt atcacaatta aaggtcaacc agccgcagta acatttgaag ccgatatcaa
    51421 cgcatttcgc ggcaagttcc tcaatgtaaa cggttattgt gactttgtag ccacaagtat
    51481 tgaggcatta tatcgtgaag gcgaaagcgc gctggatgag tggatggcgg attgtgaaga
    51541 ggacggtatt gccccttttc gtgaagaaga agagcaaaaa cgcctgacgc ttcgtgttcc
    51601 tcatcacatt gattcgcggc tgaccatcgt cgccagacaa cattccattt caaaaaacca
    51661 gctcattgtt gatgtcttag aacgtgagtt ttcagcccag atgtaaacag gtcggatgcc
    51721 gcgtggcatc cgaccttttg ccagttcgtc aatattaccc atttacaaaa tcgtctcctc
    51781 cgtcaaaatc cgccgcgcgc ctaaaaagtg atcctgccag aaaggttcag ctaactgact
    51841 tacccgaatg gtttccccgg tgcgcggtga ctcaataaac tgaccgtcgc ccagatacac
    51901 ccccatatgg tcggcaatct cgcggctgtg gatatggaaa aatagcaaat ctccccggcg
    51961 caggtcgtta ttcgccacaa tggttgcccg gcgatagtga tacatctcat tagccgtgcg
    52021 tgggagtttc gcctcaagga tcttgttgta ggcataaaaa actaacccgc tacagtcgaa
    52081 gccttgatcg ggccgcgtac cgccccagac gtaaggcttg ccaagctgtt gctccagacg
    52141 gtgaatcgcc acttcggtga tgttatgcag atggtcgctg cttaagaaat ggttgttttc
    52201 cgccagcgcc tgccagcgtc tgtcactcgc cttgagcggc gcaggaaacc attctggatg
    52261 ctgttttatc tgttcgcggt tgtgctggcg tagcgccctt ctgctttcgg cattgccttc
    52321 cacaatatag ctggcctgtt ttttcagatg ggtttggtac tgttttaata aacgcgcacg
    52381 attttgcatc cgctggcggg cggcgtagct gaaagtgata tgtgatgtgg tggcgctggc
    52441 aaatgaagaa aacggcaagc aaagcaacag taatcccgac aacacaaaag agggaatcga
    52501 cggcaaggac atactcaata atggctgatc atgcattgat gttgcagtgt atcccggccc
    52561 gtacctgaac aaacttaata aaccattatt ataaaattaa taaatatatt tcagttaatt
    52621 atgtaaaaac atccgtttat taaattactc atggattgtc gctgttgctc atttgagcta
    52681 caattcaagc tgaataaata tacagcacag gagatacccc aatggctgct aacgcatttg
    52741 ttcgcgcccg aatcgatgaa gatctgaaga atcaggcggc ggacgtactg gccgggatgg
    52801 ggctgaccat ctctgacctg gttcgcataa ccctcacaaa ggtcgcgcgt gaaaaggcat
    52861 tgccgtttga tttacgcgag cctaatcaat taaccattca atcaatcaaa aacagcgaag
    52921 ctggcgttga
//
LOCUS       DAZGJW010000016        50714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZGJW010000016 DAZGJW010000000
VERSION     DAZGJW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.016"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(108..1451)
                     /locus_tag="ACHKIK_001002"
     CDS             complement(108..1451)
                     /locus_tag="ACHKIK_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO9082218.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            1668..2591
                     /locus_tag="ACHKIK_001003"
     CDS             1668..2591
                     /locus_tag="ACHKIK_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9082219.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(2629..4269)
                     /gene="trg"
                     /locus_tag="ACHKIK_001004"
     CDS             complement(2629..4269)
                     /gene="trg"
                     /locus_tag="ACHKIK_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO9082220.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            4668..4850
                     /locus_tag="ACHKIK_001005"
     CDS             4668..4850
                     /locus_tag="ACHKIK_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFO9082221.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSRLGQARGFSPRI"
     gene            complement(4889..5062)
                     /locus_tag="ACHKIK_001006"
     CDS             complement(4889..5062)
                     /locus_tag="ACHKIK_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9082222.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(5307..5837)
                     /gene="cybB"
                     /locus_tag="ACHKIK_001007"
     CDS             complement(5307..5837)
                     /gene="cybB"
                     /locus_tag="ACHKIK_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO9082223.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            6026..7027
                     /gene="gap"
                     /locus_tag="ACHKIK_001008"
     CDS             6026..7027
                     /gene="gap"
                     /locus_tag="ACHKIK_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO9082224.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFLWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(7069..8508)
                     /gene="aldA"
                     /locus_tag="ACHKIK_001009"
     CDS             complement(7069..8508)
                     /gene="aldA"
                     /locus_tag="ACHKIK_001009"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO9082225.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPAAEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVDGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(8705..9505)
                     /gene="ydcF"
                     /locus_tag="ACHKIK_001010"
     CDS             complement(8705..9505)
                     /gene="ydcF"
                     /locus_tag="ACHKIK_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO9082226.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(9777..13679)
                     /gene="hrpA"
                     /locus_tag="ACHKIK_001011"
     CDS             complement(9777..13679)
                     /gene="hrpA"
                     /locus_tag="ACHKIK_001011"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO9082227.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRIDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            13880..14485
                     /gene="azoR"
                     /locus_tag="ACHKIK_001012"
     CDS             13880..14485
                     /gene="azoR"
                     /locus_tag="ACHKIK_001012"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO9082228.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAVPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(14536..15852)
                     /locus_tag="ACHKIK_001013"
     CDS             complement(14536..15852)
                     /locus_tag="ACHKIK_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO9082229.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(15842..17599)
                     /locus_tag="ACHKIK_001014"
     CDS             complement(15842..17599)
                     /locus_tag="ACHKIK_001014"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO9082230.1"
                     /translation="MENSRIHGEHFFTSSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTVFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(17615..18511)
                     /locus_tag="ACHKIK_001015"
     CDS             complement(17615..18511)
                     /locus_tag="ACHKIK_001015"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO9082231.1"
                     /translation="MLEKSLATLFALLILATLINHFLLWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(18511..19116)
                     /locus_tag="ACHKIK_001016"
     CDS             complement(18511..19116)
                     /locus_tag="ACHKIK_001016"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO9082232.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(19287..21593)
                     /locus_tag="ACHKIK_001017"
     CDS             complement(19287..21593)
                     /locus_tag="ACHKIK_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO9082233.1"
                     /translation="MFKSRICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNNTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(21657..22517)
                     /gene="pdxI"
                     /locus_tag="ACHKIK_001018"
     CDS             complement(21657..22517)
                     /gene="pdxI"
                     /locus_tag="ACHKIK_001018"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO9082234.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(22748..23338)
                     /gene="paaY"
                     /locus_tag="ACHKIK_001019"
     CDS             complement(22748..23338)
                     /gene="paaY"
                     /locus_tag="ACHKIK_001019"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFO9082235.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(23320..24270)
                     /gene="paaX"
                     /locus_tag="ACHKIK_001020"
     CDS             complement(23320..24270)
                     /gene="paaX"
                     /locus_tag="ACHKIK_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFO9082236.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(24371..25684)
                     /gene="paaK"
                     /locus_tag="ACHKIK_001021"
     CDS             complement(24371..25684)
                     /gene="paaK"
                     /locus_tag="ACHKIK_001021"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFO9082237.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGGAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(25711..26916)
                     /gene="paaJ"
                     /locus_tag="ACHKIK_001022"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(25711..26916)
                     /gene="paaJ"
                     /locus_tag="ACHKIK_001022"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFO9082238.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPHSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(26916..27338)
                     /gene="paaI"
                     /locus_tag="ACHKIK_001023"
     CDS             complement(26916..27338)
                     /gene="paaI"
                     /locus_tag="ACHKIK_001023"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFO9082239.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(27328..28755)
                     /gene="paaH"
                     /locus_tag="ACHKIK_001024"
     CDS             complement(27328..28755)
                     /gene="paaH"
                     /locus_tag="ACHKIK_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFO9082240.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(28757..29545)
                     /gene="paaG"
                     /locus_tag="ACHKIK_001025"
     CDS             complement(28757..29545)
                     /gene="paaG"
                     /locus_tag="ACHKIK_001025"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFO9082241.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(29545..30312)
                     /gene="paaF"
                     /locus_tag="ACHKIK_001026"
     CDS             complement(29545..30312)
                     /gene="paaF"
                     /locus_tag="ACHKIK_001026"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFO9082242.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATD
                     TSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(30309..31379)
                     /gene="paaE"
                     /locus_tag="ACHKIK_001027"
     CDS             complement(30309..31379)
                     /gene="paaE"
                     /locus_tag="ACHKIK_001027"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFO9082243.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGERLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(31387..31884)
                     /gene="paaD"
                     /locus_tag="ACHKIK_001028"
     CDS             complement(31387..31884)
                     /gene="paaD"
                     /locus_tag="ACHKIK_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFO9082244.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(31899..32645)
                     /gene="paaC"
                     /locus_tag="ACHKIK_001029"
     CDS             complement(31899..32645)
                     /gene="paaC"
                     /locus_tag="ACHKIK_001029"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFO9082245.1"
                     /translation="MNQLTAYTLRLGDNYLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGIDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(32654..32941)
                     /gene="paaB"
                     /locus_tag="ACHKIK_001030"
     CDS             complement(32654..32941)
                     /gene="paaB"
                     /locus_tag="ACHKIK_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFO9082246.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(32953..33882)
                     /gene="paaA"
                     /locus_tag="ACHKIK_001031"
     CDS             complement(32953..33882)
                     /gene="paaA"
                     /locus_tag="ACHKIK_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFO9082247.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            34167..36212
                     /gene="paaZ"
                     /locus_tag="ACHKIK_001032"
     CDS             34167..36212
                     /gene="paaZ"
                     /locus_tag="ACHKIK_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFO9082248.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            36460..38733
                     /gene="tynA"
                     /locus_tag="ACHKIK_001033"
     CDS             36460..38733
                     /gene="tynA"
                     /locus_tag="ACHKIK_001033"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFO9082249.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDETL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(38791..40290)
                     /gene="feaB"
                     /locus_tag="ACHKIK_001034"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(38791..40290)
                     /gene="feaB"
                     /locus_tag="ACHKIK_001034"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFO9082250.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            40526..41431
                     /gene="feaR"
                     /locus_tag="ACHKIK_001035"
     CDS             40526..41431
                     /gene="feaR"
                     /locus_tag="ACHKIK_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFO9082251.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGHLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(41603..41932)
                     /locus_tag="ACHKIK_001036"
     CDS             complement(41603..41932)
                     /locus_tag="ACHKIK_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO9082252.1"
                     /translation="MMKKTLLFCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(41937..42122)
                     /locus_tag="ACHKIK_001037"
     CDS             complement(41937..42122)
                     /locus_tag="ACHKIK_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO9082253.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(42119..44758)
                     /locus_tag="ACHKIK_001038"
     CDS             complement(42119..44758)
                     /locus_tag="ACHKIK_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO9082254.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNSEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            44966..45955
                     /gene="ldhA"
                     /locus_tag="ACHKIK_001039"
     CDS             44966..45955
                     /gene="ldhA"
                     /locus_tag="ACHKIK_001039"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO9082255.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            46066..46488
                     /gene="hslJ"
                     /locus_tag="ACHKIK_001040"
     CDS             46066..46488
                     /gene="hslJ"
                     /locus_tag="ACHKIK_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO9082256.1"
                     /translation="MKKLAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(46485..46751)
                     /locus_tag="ACHKIK_001041"
     CDS             complement(46485..46751)
                     /locus_tag="ACHKIK_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO9082257.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            47025..50549
                     /gene="nifJ"
                     /locus_tag="ACHKIK_001042"
     CDS             47025..50549
                     /gene="nifJ"
                     /locus_tag="ACHKIK_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO9082258.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGSWPMGTTRWEKRNIAEEIP
                     TWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT        12710 a        13466 c        12126 g        12412 t
ORIGIN      
        1 ttgaactcag agaatatgca taaatcggca atttttttat gtcgatggga tgagcctgat
       61 tcgtttgtaa aatcaggcaa atttttgcaa aggcggatgg tcagaattta aagcaaacca
      121 cagcgacgtt tactgcgttc ttccgcttcc tgataaagct ggaactcatc caaaaccgga
      181 caacccaacg cctgctcaaa tgcctcgcga ctggcattac cgtgattgcg tgcctgcgtt
      241 tcattaccca gatttgactg gaagatcccc gccgcgctaa cgggcaagaa atcttcatag
      301 gtaatgggtt gcgccgcgac ccaaccacgt tcaattaagg gctgtggatc gtctccggga
      361 tgaatcgcct gacgatgcgc ctcacctgaa ggcgtcagac ggtaccggaa ccatgccagc
      421 ccttgctgac gcattaaaaa ctcactgtca ggaaaagtgc ggaaggtttc ctgtaaatgc
      481 atttggtgag tgagattatc ctgcccggtt ccagcgttac gcagaagatc atcatacagt
      541 tgtcgccctt tcggcgttaa tgccacgcca cgctgctcaa tttcaccaaa gcgcgcggta
      601 tgcgtgccct gtttctgccc cgcaaacaac accgtctctt ccagtgcttt aaagctggtc
      661 tggcgtagta aaatcggtac ctcgcggcgc ggcggcccct cgatgagaat tttgggttca
      721 attccgcatt caggcatcat cgactgcacc cggtcaatat ccagcgtacg tggcgtcagg
      781 tggttgatat ggcatccagg aaaacagacc acatcagcaa tcaaccgatg ttcgttgtgc
      841 aaggcgcgat aggtttcttc atctaccgtt gctgactggt gccagcgaaa cgtttccagg
      901 gcttcctgca caaactcctg tgcctgtgtt tcgttaaaac cgccctgctg ctcatattcc
      961 tctaacagtt gtcgacaacg tggggtgaag atatcgcgct gacgcagaat ctccgccgct
     1021 ttctggcgca aaatttcgtt ctcgataagc tcaaggcgga gtaaggaggt aaaaacgcgg
     1081 aagggattac gcgccagaga agcatcatca atgggccgaa atgctgtcga atgtaccggc
     1141 acccctgcct gcgagagatc gtaatagctc accgggtaca tccccataat ggcaaacatc
     1201 cgccgaagag tagcaagctc ttgtgcagtc ccaacgcgaa tcgccccatg acgttcaaca
     1261 ttcagtcgcg ccagctcgtc tgcatttacc attttttcgt gcagttgagg attgttttcc
     1321 agcacagcca gattcacatc agctaccagt tccagcagcg tgccatattg cggaacttct
     1381 tgctggtaca tggctgacat tgcctgcgaa aactgttccc gaatctcatc cgccgtgatg
     1441 ctgttcgcca tagtgtctgc ctccagtggt cagtaatctg gagtgtaggt aaccgcattc
     1501 actctggcgg gaagaattta caaactgtga tctcgccgcg aaaacatcaa tattatccat
     1561 tttgctgtaa cacaattgct ttaattgtta ataatatttt gcaatcaagt tatcataatc
     1621 aaacaacttc acttgtcagc gacaccgctt cgtttttaac atcgcttatg gaaaaaaata
     1681 gtctgtttag tcagcgcatc cgtttgcgcc accttcatac attcgtagct gtcgcacaac
     1741 aaggaacttt ggggcgcgcg gctgaaaccc ttaatttgag tcaacctgcg ctctctaaga
     1801 cattgaatga actggagcag ctgactggcg ctcgcttgtt tgagcgtggt cgtcaggggg
     1861 cgcaacttac cttacccggc gaacaatttt taacgcatgc agtcagagtt cttgacgcca
     1921 tcaacactgc cggacagtcg cttcatcgta aagaaggtct taataatgat gtcgtcaggg
     1981 ttggtgcact acctactgcg gcactgggga tattaccttc ggttataggt cagtttcatc
     2041 agcaacaaaa agagacgacc ttgcaagttg cgacaatgag taaccctatg attctggcgg
     2101 gtttaaaaac cggggaaatc gatatcggca ttggtcggat gtcagatcct gaactgatga
     2161 ccgggcttaa ttacgaactg ctgtttcttg aatcgttgaa gctggttgtc cgccctaatc
     2221 acccgctact tcaggagaac gtaacgctaa gccgggtgct ggaatggccg gtcgttgtat
     2281 caccagaagg cactgcgcca cgccagcatt cagatgcatt agtgcagagc cagggctgta
     2341 aaattccttc gggttgtatc gaaacgctgt ctgcttcgct atctcgtcaa cttacggttg
     2401 aatacgatta cgtgtggttt gtcccttctg gcgctgtaaa agacgacctg cgtcatgcca
     2461 cgctggtggc cctgcctgtt ccgggacatg gtgcaggcga accgattgga atactgaccc
     2521 gcgtagatgc gacgttctct tctggttgcc agttgatgat taacgctatt cgaaaatcaa
     2581 tgccgttctg aaaggtgaag ggatctgtcg atccctcctt gaacattttc acaccgtagc
     2641 gaaactaact ggttcacccg ctccgcgagg ttctgccgac acagaatgtt tgtgcagacg
     2701 gaatacatcc accgcctcag ttaatcgtgc cgcctgttct tcaagggaca ccgccgccgc
     2761 tgaggcctct tctaccagag aagcattctg ttgcgtcacc ttatccattt cagaaatcgc
     2821 ctggctaacc tgcgttatgc ctctactttg ttcatccgag gcggcggcga tttcctgcat
     2881 gatatgtgtg acactcgcga cggcatcaac aatagtgctc atggttttcc ctgccgttgc
     2941 cacctcatcc gaccccaggt caattaacct gactgattca ctgatcaagc cttcaatctc
     3001 tttcgccgct tgggcgctgc ggcttgcgag tgtccgtact tcgctggcga caacggcaaa
     3061 tccacggcct tgctcacccg ctcgcgcggc ttcaacggca gcattcagtg ccagaatatt
     3121 cgtctggaaa gcaatactgt tgatgacggc ggtgatctcg gaaattttct tcgaacttgt
     3181 agagatagcg cccatcgttt ttactacacc ggaaaccgtc tgcccgccat cgctggcttt
     3241 aatagaagcc tcttgcgcca gtttgctggc atgatgcgcg ttatccgcat tctgtttcac
     3301 cgtcgcagtg agttgctcca tgctagcggc agtttgttcg atagccgccg cttgttcttc
     3361 ggtgcgagat gacaggtccg cattgccagc tgaaatttcg ctggtgccac gataaatctc
     3421 ttccgcaccc tgtcgaacag tccctactgt catccccagt gaatgctgca tttgctgtaa
     3481 atgacgactt aagcgaccga tttcattacg acccgccggt tcatcattca tcgtcagatc
     3541 gccactggcg attttttcaa tccgttgtgc ggcatgttgc agtggacgaa tgacgatccg
     3601 acgtagcacc ataaatgtta tcagcgtcat gaccagggca agcacaaacg cgccaatcat
     3661 gaacatccca cccaggcggg tgcgctgatg ggccagttcc gccagttggt tggctctggt
     3721 gctacgtatc ttaacggctt tgttcaaaat atcggtataa gcattatcca gcggtcggat
     3781 ctgctcactt tcatgattga taatcgcttc aaacatgcca tttttggcat atttcaacat
     3841 aggttgcata cccgtgatat aagcctgaaa gcgttgattt aattcagtgt cgagggcttc
     3901 atcagcaggt gttttcaccg gtcgattctg ataagcacga taaccttgct gcgactgttt
     3961 aatctccgat tcggcttgcg caatatttcg cttcattgct tccatttccg caatacgact
     4021 tgcagccccg gcctgaatca tattgatccg ggctgaacgc aaaaaatctg aactgttcgc
     4081 taacccggtc cgaatctcaa tctccgcagt aacatcacgc tgatcgcgat cggcctgcca
     4141 gaggaaatag ccagccagag cagaacttaa tgcgaataga actaagatac cgccaagaat
     4201 gcaggcaaat aacggaacca gcctgatgtg atgcaaaaaa cctaatcgat gtgagggagt
     4261 tgtattcatt accgttgtct ctcgtccagg tttactcctg atagaaagtc atcggcactt
     4321 gacggtaatt acttatgacc aaaaacctct ttgcgtcaca tttttacaac aattattgca
     4381 gagaaaatta atgctattgg aatgaagaga gtaacgaatg gctaaactgg ctaacccgaa
     4441 tgacgaaaaa ttctggaaaa cagcacgcaa tttcgtgaag tgattatttt cgtccctaag
     4501 gaaacctcat aggatgcctg cgggtgcctg agactttctg ttttcaggct aaccgggaat
     4561 cagaaagacg aaggccccga ccgatatttc tatcaaccga ggccaacgaa tgaacctagc
     4621 aattcagatc ttagcctctt acccgccatc cggcaaggag aaaggctatg aagcacaacc
     4681 ctctggtggt gtgtctgctc attatctgca ttacgattct gacattcaca ctcctgaccc
     4741 gacaaacgct ctacgaactg cggttccggg acggtgataa ggaggttgct gcgctcatgg
     4801 cctgcacgtc caggttaggg caagcgcggg gattttcccc gcgcatctga ctagcagatt
     4861 cattttctca tggcacccct tttacacttc agttagtgca tgtttttttc gaggcactaa
     4921 tagaaccatc attacagaca aatttgccgc ctgccgtaca gtgtgagatc ccccctttcg
     4981 aaccagaaca gggtttgcgc ccggcatcgg cataaaagga aacaagcgtt cccataaaca
     5041 aaataagtgc cagttttttc atataacctc catggtttat cgaatcttat gttcgatttg
     5101 aacgttatac gctggtttat tttgataccg ttgatgagat caaagatcgg gtgaattgat
     5161 taaaaataag ccagatggac gtatgggcaa ggattatggt tttatttgtc atacaaataa
     5221 atataatagg cgcttccgat gtagacccgt attcttcgcc tgtaccacgg gtcggtttta
     5281 gtacaggcgt tttctttaaa tatccttcag gaacgtttac gcggcatcat gcgtagaagt
     5341 gtgttgtcct tccagaaata gtggtgtgcc agtgcagccg cagcgtgcaa cccgatgaca
     5401 aaatatccca gattcgccag cgtctcatgc cacgacttta agctatcaac ccgttcgaaa
     5461 ttggcctctg aagcgtaagg catcgtcaaa ccaaacgcaa accacgggtt gccccggtta
     5521 tacatcatca ccaaaccaat cactggcagc gcaataaaca ggagataaat caccaaatgt
     5581 cccaaatgcg ccagtcccgt catcatcggt tttggcttag gtataatcgg cggggttggg
     5641 tatttcagtc ttaacaaaag acgaaccacc atcagcacga gaattgagat accacaggaa
     5701 acatgaacca tgttgatgag tggccgatcg ctacgtggga agaaaccacg aaactccatt
     5761 gcgcaatacg ctgcgataac cagcaaaaag accagccagt gaatgctgat ttgtaacctt
     5821 gagtatttat tttccataac atttcctgct ttaacataat tttccgttaa cataacgggc
     5881 ttttctcaaa atttcattaa atattgttca cccgttttca gtacgttacg tttcactgta
     5941 agaatggaga ttgcccatcc cacaatcctg gtctaagcct ggaaaggatc aattttcatc
     6001 cgaatgttcc tgacaggaga aaaccatgag taaagttggt attaacggtt ttggtcgtat
     6061 cggtcgactg gtgttgcgtc gattacttga agtcaaaagc aacatagacg ttgtcgctat
     6121 taatgatctc acttccccaa aaattctcgc ctacctgctg aaacatgatt caaactacgg
     6181 accattcctc tggagcgttg attttacgga agattcactt atcgttgatg ggaaaagtat
     6241 cgcggtttac gccgaaaaag aggctaaaaa tattccgtgg aaagcgaaag gtgcagaaat
     6301 cattgtcgaa tgtactggct tttatacctc cgccgagaaa tcgcaggcgc atcttgatgc
     6361 tggtgcgaag aaggtgttga tttccgcccc tgccggtgaa atgaaaacta tcgtttataa
     6421 cgtcaatgac gacactctgg atggcaacga caccattgtt tccgtggcgt catgcaccac
     6481 taactgtctt gcgccgatgg ccaaagcctt gcatgacagt ttcgggatag aagtcggcac
     6541 gatgacgacc attcatgcct atactggcac ccagtcactg gtggatggcc cacgtggtaa
     6601 agatttacgt gcttcacgcg cagcggcaga aaatatcatt ccccacacta cgggggcggc
     6661 aaaagccatt ggtctggtga tcccggaact gagcggcaaa ctgaaaggtc atgcgcaacg
     6721 cgtgccggtg aaaacaggtt cggtcactga gctggtgtcc attctcggaa aaaaagtgac
     6781 tgccgaagag gtgaataacg cacttaaaca agcaaccacc aataacgagt catttggtta
     6841 taccgatgaa gaaatagtct cttccgatat cattggcagc catttcggtt cggtgtttga
     6901 tgccacgcaa acggaaatta ccgccgtggg cgatttacaa ctggtgaaaa cggtcgcctg
     6961 gtacgataac gaatatggct tcgtcacgca gcttattcgc accctcgaaa aattcgctaa
     7021 actctgacgc gcacaggcgg aggaaaaaac ctccgcctct tttactcatt acgactgtaa
     7081 ataaaccacc tgggtctgca gatattcatg caggccatgt ttaccatctg cgccgccaat
     7141 accggattta cgccatccgg cgtggaagcc ttgcatagct tcgaagtttt cacggttgat
     7201 gtaagtttca ccaaacttca gccctttaat ggctttcatc gcgacgttca gattttgggt
     7261 atagattgat gaggtcaggc cgtaatcact gtcattagcc attgagatag catcttccag
     7321 cgtgtcaaat gcgaccaccg gcagaaccgg gccaaaggtt tcctcatgca taatcgacat
     7381 ttcctggcga acatccagca gcaatgtcgg cggataataa tatcctttcc cgtctactgc
     7441 tttgccaccc aacgccactc tcgccccttc ttctactgcg cgcgccactt tttgctcgac
     7501 cctttccagc gccgcggcgt taatcaacgg ccccatcgca atgtcgttgc gttcagcggg
     7561 gttaccaaat tgaaccgcct gcatcgcttc acccagccga ttgacgaact gatcataaat
     7621 gcctttctgt acataaacac gttctgcaca gttacacact tgcccactat taatgacgcg
     7681 tgaatcaacg atggctttga ctgccagttc aagatcggca tcgtccatta cgatagctgg
     7741 tgctttacca cccaattcca ggcacacttt ggtgatgttt ttcgccgcag tcgccataat
     7801 cttctcacct gcagagacgc tgcctgtcat actgaccatt gcgacctttg ggttacccgc
     7861 cagttcttgc ccaacggttt caccacgccc cagtacaagg ttaaacacgc cgcgcggaag
     7921 gcctatttca tcgacgattt tggcgaatgc aatcgcattg tttggcgtaa attcactggg
     7981 tttaatgacg atggtattac cggtcaaaag agcgggagcc attttgcggg caatgaggaa
     8041 gaacgggaag ttccacggca gaatgccggt agtcacacca agcgcacgtt taaacaaaag
     8101 aatattttct cctggacgat cgctttgaat aatctcgccc tcgtaacgcc gtgcccactc
     8161 cgccatgtaa tcgatatagt cggcagtaaa agccacttcg acttcagcca gctgctggat
     8221 cttgccccct tcttcaacaa tcagcgcact gatttcactg gcgcgttcgc ggatgccggc
     8281 ggagattttg cgcaaccaac tggcgcgttc aatagcaggc aacgcttccc attctggttg
     8341 tgcacgttct gctgcatcga ttgccttacg ggcatcctcg gcctgaccat cgggtatgcg
     8401 ggaaatgaca gcctctgcag cagggtttac cacatcaatc catgcgtctc cacgccaggt
     8461 aacaaactgt ccatcgatat acataggatg ttgaacgggt actgacatgg gcgactcctg
     8521 tgatttatat gtttgttttc ggtgaatgca ttgttaatta acatcaatca tcagagagac
     8581 ggcatcgtga aaggacaatt ctgtgaaggg cttcataaaa acaagtgtaa aaggaataaa
     8641 aggcagaaat aaaagtcagg agtcatgccc gtttgcaaac aagtggttca caaacgggca
     8701 atttttaacg taacgagcga ctttccatcg cctcgatgag caccgcatca tgtttcagcg
     8761 tttgccatgc atggatgact tctgccggaa aatcaacgtg aacgataaaa tctcgcccgc
     8821 ggggaccgta gccatcgcta tcatcgcgta aacgcggcag ctcgccagtg agtagtgaga
     8881 gataacgctc aaccggccat aatccttgta actgattaat aaagattaca ctgtctgcgt
     8941 tatttcctaa ctgaggaacg aatccgggat aacttaacca gcgtggtgca tcgggattgt
     9001 ccccagtcat acggcggaac gtcgccatcg tgcgccgctg catggtgggg tcctgaacaa
     9061 cgatagccgt atgaactcgt tctacggcct gattcaatag cgcgatgcta aagcgtgcgt
     9121 tttcaccgca gtttgttgac tggtcttcaa tccagatttt ttcatgcgga atgtgccaga
     9181 actgatgagc gatatccgcc aggatggtcg cttctgctct gccagtggtg cggatagtgt
     9241 tgtagtgcgg atgctgtgcg atggcgctat acaaaaaagt tgtcgagtga ccgataccac
     9301 cactaatcag taaaggaatt tgctgatcgc gagcaatctt acatgccgcg tcgatagtcg
     9361 gcataaccgc attgcctgca aggatcacgc aatcggcctg atacggcacc tcaccggaga
     9421 aatcatcctg cgccagccac tgtccgataa catttatggc atctatagtt gccggcgaaa
     9481 gcgtcggaaa cggggtaatg ttcatggttc ctccttatcg ttcccttaag aatactgcgt
     9541 gagataatag tgagaagcgg agcactctga aaggaggaag tcgtatgtcc ttaaccgcca
     9601 ctccgcaagc ctgacatatc ttttaaggaa atgctgtaga taaacattat tcatcttgca
     9661 gcagagaatt gtatttttct gaattcctga atttttaccc ttaccgtttt agaattaata
     9721 aaaaacccgg cgctaaatgc ttaccgggtt ttttctttat caggcaaata gcagggttaa
     9781 ccgctaatct gctccatcgc ctgcaaaata cgcttatctg aaatcggata aggcgtacca
     9841 agttgttgag cgaagtaact aacgcgcaac tcttctatca tccaacggat ctctttcacg
     9901 tcttcatcct cacgacgtgc gggcggcagt ttgttgatcc attgctgcca cgcctgctgg
     9961 acgttttcga ctttcagcat ctgagcacgg tcgcgatgtg gatcaaccgc cagtttttcc
    10021 agccgttttt caatcgcctg caaatatcgc agcgtgtcgc ccagccgttt gaagccgtta
    10081 ccagtgacaa aaccgcgata taccaacccg cccatctgcg ctttaatgtc agaaagcccc
    10141 agtgccatgg tcatatccac ccgccctttc agacgtttgt tgatattgaa caccgcagtg
    10201 aggatttgct cgacctgctt cgcaatatcc accaccgtgt cattcagttc ggcgcgcact
    10261 ttttcatgca gcgcagcaaa gccttcttcc gtccagaccg ggccaccatt ggcgtcgatc
    10321 aattgatcca caccgcagga gatacagtcg tcgatcagct ccagcacttt gccatacggg
    10381 ttaaagtaca gtcccagctt ggctttgttc ggtaactttt catgtaaata tttgattggc
    10441 gatggaatat tcagcagcag tagacggcga agaccgttcc acattgcctg cttttgctcc
    10501 agcgggttat caaacagttt gatcgccaca ctgtcgcgct catccaccag cgccggccac
    10561 gccttcactt tgtagttgcc acgcttctgt tcgtagcttt ccggcagctg accaaaactc
    10621 cagatatgta agccgctctg ctcgataccg tcatccgcca ccgcagatag cgtttcctgc
    10681 actttgcctt tcagcgcatc tttcagatct tgtagcgagc gcccttcttt tagcttcttg
    10741 tttttgtcat ccaccacgcg gaaggtaatt ttcaggtgat caggcacctg atcccagtgc
    10801 cagtcttcgc ggtcaacggt aacgccggtc atccgccgta actcgcgctc aaggctgtcg
    10861 agcaacggta actccagcgg tttgacgcgg cctaaaaacg cttcggcata gtttggcgcg
    10921 ggtacaaaat tacggcgtac cggtttcggc aacgatttaa tcagagcaat caccagttcg
    10981 cggcgcagac cggggatctg ccattcaaac ccgctttcct caacctggtt aagtaacggc
    11041 agcggaatat gtacggtcac accgtcagca tccgcgccgg gctcaaactg atagctcaaa
    11101 cgcagcttga gattgccctg atgccagaag ttcgggtaat ccagcttgct gattttttct
    11161 gcgccctctt tgatcaacat gcttttttca aagttgagca aatcaggggt ttcgcggctg
    11221 acttttttcc accagctgtc gaagtgacga gcggagatta catcgtggct gatgcgctgg
    11281 tcgtagaact caaacaacgt ttcgtcatca accagaatat cgcggcgacg tgatttgtgt
    11341 tccagctctt ccacttcggc ccgcagtttc aggttttcac ggaagaatgc gtgacgcgtc
    11401 tgccagtcac cttccaccag cgcatggcga ataaagagtt cacggcataa cgccggatcg
    11461 atctggctgt agttgacctt gcgcgcggca acaatcggca aaccataaac agtgactttt
    11521 tccgttgcca tcaccgcgcc ctgcgcccgt tcccagtgag gttcgctgta ggtgcgttta
    11581 atcaaatgct gagcgacagg ctccacccat tccgggtcga tacgcgcagc aatgcgcccc
    11641 cacaggcggc tggtttctac cagttccgcc accattaccc atttcggcgg ttttttgaat
    11701 aaaccagaac cgggaaagat ggagaaacgc gcgttacgtg cgccggtata ttcttgttta
    11761 tcggcatctt tcatgccgat atgggaaagt aaacccgtca gcaacgcaat gtgaatttcg
    11821 cgatactccg ccggttcgct gttaaccgga atgccaagtt ctttcaccac ctggcgcaac
    11881 tgggtgtaga tatcctgcca ttcgcgcacg cgcagatagt tgagataatc gatacgacac
    11941 aggcgacgga aggcgttgga agaaagcgcc ttttgctgct cgccaagata attccacaga
    12001 ttcacaaacg cgaggaagtc agactctttg tcgtggaagc gacgatgttt ttcgtccgat
    12061 gcctgctgtt tatccatcgg acgctcgcgc ggatcctgaa tggaaagcgc ggacgtgata
    12121 atcatcgcct cacgcacgca gccatgtttt tgcgcttcca gcaccatacg cgccagacgt
    12181 ggatcgacag gcaactgcga gagctggcga ccgagcggcg tcagtttata ggcgctggcc
    12241 tgttcatccg tggtgatcgc gcccagttct tcgagcagac gcacgccatc ctggatattg
    12301 cgtttatccg gtgcttcgac aaacgggaac gcagcgatat cgcccagccc cagcgcggtc
    12361 atctgcaaaa taaccgaggc caggttggta cgcagaatct ccggatcggt aaactccggg
    12421 cgcgaaagga aatcgtcttc ggaatagaga cgaatacaga tcccttcgga cacacgccca
    12481 cagcggcctt tacgctgatt ggcagacgcc tgcgaaatgg gttcaatcgg caaacgctgc
    12541 actttggtgc gatagctgta gcggctgata cgtgctgtac cggggtcgat aacgtattta
    12601 atccccggta cggtcagcga ggtttccgcg acgttggtcg ccagcacaat gcgccgtccg
    12661 ctgtgcgact ggaacacgcg gttctgttcg ctgttcgaaa gccgcgcata aagcggcaag
    12721 atttcggtat gacgcagatt tagcttgttc agcgcatcgg cggtatcgcg aatttcccgc
    12781 tcaccactca taaagatcag aatgtcgcca gggctttcct gactcagttc gtctacggcg
    12841 tcaaaaatcg cctgcaactg atcgcgctcg gtgtcgtcgg cttcttcaac aatcggtcga
    12901 tagcgcactt ccaccggata ggtccgaccg gagacttcga taatcggcgc attattaaag
    12961 tggcgcgaaa agcgttccgg gtcgatagtc gcggaagtga taatgatttt taggtcagga
    13021 cgccgcggca gcaactcttt caaatagccg agcagaaaat cgatattcag gctgcgttcg
    13081 tgtgcttcgt caataatgat ggtgtcgtac tgcatcagca ggcggtcttg ctggatctcc
    13141 gccagcagga taccgtcggt catcagcttg accatcgtgt tatcactaac gtgatcgctg
    13201 aaacgcactt tgtaaccgat gcaaccgccc ggctccgttt tcagctcttc cgcaatacgg
    13261 ttcgccaccg ttcttgccgc caggcgacgc ggctgggtat ggccgatcag ccctttaatc
    13321 ccgcgcccca gctccataca gattttcggt aactgagtcg ttttaccaga acccgtttcc
    13381 ccggcgacga tcaccacctg gtgatcacga atcgcttcga gaatgtcctg ttttttctga
    13441 ctaaccggta aattgtcagg ataagtaatt tccggtcgtg ccgcttcacg cagcaggact
    13501 ttccctgccg cctggtcaat ctctttcgcc atctcctgga aaatggcctg ttgtgcatca
    13561 ggatttttaa ccttcttaac accgtgcaga cgccgagaaa aacgcagtct gtcgcgcagc
    13621 atcagcgaat ccagccgctg ctgcaaggcc gtaaaggtca atttttgttg ttctgtcata
    13681 acgttagtgg gcagtagtct gcctgattaa ttttctttta aatgatagat atagagtacc
    13741 acatcgcggc tttctatgcg ttgttcaata aattcgaaca taggcttcga tttattgcgc
    13801 tatctctgtg gcaggattgt gaataaagtg tcagcaagca acggggcatc gcccattcaa
    13861 acatctataa ggaaacacca tgagcaaggt attagttctt aaatccagca tcctggcagg
    13921 gtactctcag tctaatcagt tgtccgatta ttttgttgaa caatggcgcg aaaagcactc
    13981 cgctgatgaa atcaccgttc gcgacctggc tgcaaatccg attccggtac tggatggcga
    14041 actggttggt gcactgcgtc cgagcgatgc gccgctgact ccgcgtcagc aggaagctct
    14101 ggcgctttcc gatgagctga ttgccgagct gaaagcccac gacgttatcg ttattgcggt
    14161 accgatgtat aacttcaaca tctcaactca gttgaaaaac tattttgacc tggttgcacg
    14221 cgcaggcgtt actttccgct ataccgagaa cggtccggaa ggtctggtaa cgggtaaaaa
    14281 agccatcgtt attaccagcc gcggtgggat ccacaaagat ggaccaacgg acctggtgac
    14341 gccgtatctg tccacgttcc tcggctttat cggcattacc gatgtgaaat ttgtcttcgc
    14401 cgaagggatc gcatacggtc cggaaatggc agcgaaagcg cagtctgacg cgaaagcggc
    14461 catcgacagc attgttgctg cataaccgga cacgatcttg atgtggactg tagttcagtc
    14521 cacatctcaa tccacttacc tgttttccca taatttcagc atcgctttgt gatcttcctt
    14581 aagcacaata tgcgaacgtc tggctcgaat ataactaatc gcttcatcaa cggttttaca
    14641 gtgtccgtaa cataacaacc atgccgccac taccagcgca ctgcgcgata atcccaacgc
    14701 gcaatggacc agaacgctgc cttgctcttc gcgtaatgtt tccagcatcg ccacggcctg
    14761 tcggagctcc ccctcttccg gaaccaccag atccagcatc ggtacacaga aatagagtcg
    14821 atcttttgtc gctcgtcccc gagggaattc aaaggtgacg tccagaaccg cattctgtgc
    14881 cggaatatgt cgtggaaacg cccctaaata aacaccagca gtaattttgc tcaccggctc
    14941 caggcgacga caaaaccaac gcatagacag ccacatcccg atgcgccagg gcaatgtcag
    15001 ccagtaaacg gcgggcggga gtttcccctg actatctttc cctgttgtta tcgcgccaag
    15061 cccaccgtac ccacggccaa taatgagtag cgataatact ggccaacata accagactga
    15121 ccaccataac tgaatcatca ccattagctc catcaacaca atgcacgagc ccgcgcctac
    15181 gacatatggc agtgctattt tgattcgacg ttgatcaggt ttctgataat tccaacgacg
    15241 atcgacgggc accatccagt caatcaacat acctaccgcc agccctgtga tgacatcaat
    15301 aaaatgatgc tgccaggtcg tcagagtaga aatagcgatg agtaaaaacc atccgccaca
    15361 gactttacgc cacctctcag ccagatgctg acgaaagtga cgccaaagta gccagcagag
    15421 aataatatgc agcgaaggag actggttata aggcagatca aacagttcaa gttgcgaaaa
    15481 tagccatccc gtcaccccac tcacttcagg acggataaaa ctaaacttca gcggatagag
    15541 caaaaaaccg cagcaggcca ttaccgttgc cagaataagc cggtggacaa gtcggcgctg
    15601 ttcgaatgtc gtgctacaaa cgaacagcga aaatccatat aaaagatcca gactccagta
    15661 aggaacaata gtccagggaa gaaaagggat cgccgtttcc caaccgaata cctgactggg
    15721 gatatcatgg ctgttaaggt cctgaaccgc ggtgaactga ttaagagatc cataggtgaa
    15781 gaagaaaaac ggggccagca acaataacca gccagcgcct tgtagcaata cgttccgacg
    15841 ttcagttatc acgacgcacc gccatcgaaa ccgtaaaaat accccactca tcaatccgtt
    15901 gtgtgcattt atcaaatccg gcatcacgca cgagtgaatc catctcccct tgcgaacgca
    15961 cgcgcattac ccacggttta ccatctttat gactggttaa caccccggca atcatctcca
    16021 gttgagggtg ccacggctgc ccggtgtaga tgagaatgcc gcccggttcg atggcattgg
    16081 caagacctgc gagtgagttt tttacctgct cattttcggg aaaaagctca tacaggccag
    16141 agacaatcgc cagcgtaggc cgcggagtta acgcgctgag ttcctccggg ttaaacgcat
    16201 cgccctgttc aaaacgcacc cgcccagaca ttccccgttg ggcaatcatc gcttgcccct
    16261 gtgcaacatt taactcactg taatcacgta acaaaatatc gcttacggca ggctcgtttg
    16321 ccagcgcatc cagtacatag cgcccatgcc ctgcggcaat gtcaaccacg cggacggcta
    16381 aacctttggc gtggagatcg gcaacggctt gtttaatcag tatttgtaaa tgggttttac
    16441 gctggcgaat accgcgccag ccaacactgt tcaggtagat tttgtcaatt aagcgcccga
    16501 atgcgttact gccttgcggt tgattttgat agacataatc cagggaactg cctgaatcaa
    16561 agccggtgct cattccgaga tgcaggcccg cagagtgcgc cccgaacaat ttcatcgcct
    16621 ttcgcataaa gcgatacgcc aaatcaaccg gcgataatgg cacgggtcca cctgaaagga
    16681 gccgccagcg atccgctgat ggtccagtgc ggtcttcatg ctgataatca aatttttgtg
    16741 acttgttagc atataaacga ctaataaagc tttgcatttt ttcaaatgcc tgcgccctgt
    16801 tctcttcacc caacgtgtcg tgataaaagc ctggcagcag atgcagctct ttcagagggc
    16861 tacgtaatct ctgataaaaa tcaatctgcg gttggcgatg caccacatag tcatcgcctg
    16921 atatcagaag ttgcgtgggg agcgtaatcg ccgccgcatc tctaataata cgttcagacg
    16981 ttttgtagag atcgagcaag atattaacgg caatcgcccg tgtgatcagc ggatcattat
    17041 tgaaactcgc cacccgttgc cgatcgtggg tcaaatagcg tcctttcaca taggaattaa
    17101 taaaaaacag accacgcaga cgatgccata acgccagcgc aggacgtgcc agcggtacat
    17161 acaatttaac cttaaaggcc ggagaagcca gcaccagccc gcgaattgca ggcgcataat
    17221 catgaaccca tgtggcaacc agcactgcgc cgacgctttg cgcgatcact accacctcat
    17281 ccagtccgac ctggctatcg ctggcagcaa aacgaacaaa ttcatcgaca tcctgcactg
    17341 aacgcgcaag agatgggcta taaccacgcg gccccgaagt ttgtccatgc cctcgggcat
    17401 cccaggcata aaaaacagta tctggcatcg ccagttcatc aacgagatgt tgtagacgac
    17461 cagaatgttc atgaccgcga tgaaataaga cgatgacctt tttagccccg ggctgtaaag
    17521 cgggccagtg ccgataaaac aacgctgtat tatcactgct ggtaaaaaaa tgttccccat
    17581 ggatgcgtga attttccatt ttctctattt tccttcagta acagcagtag cgaataaaat
    17641 aaaaaaatac cggagcggtg aaaattaatg agtcaatcct gtcaaggagt ccgccatgtc
    17701 ctggtaatag tttcccacta tctttaacac caatatctcg tttgatggct gacatcacga
    17761 catcgccgca aaaaccacta ataccaatta acaaacccgc taataatgcc tgtaatgtat
    17821 ttagcggtgt cagcaacggt ccaataatca gtgacgcaat catggtggtg attacgcccc
    17881 ccaccagacc ttccagcgtt tttcccgggc tgactttggg gaccactttt cttctgccgc
    17941 aggatttccc ccataaatac tgtgcaatat cgtttgactc ggttaatgcc aaaagaaata
    18001 acaccagtaa cgccccgccc tggatatttg tggttggcaa cattaataac caggcggcat
    18061 gactcaaggc gaaaacggtc attatccagc cccaaaaaat ggcgctcacg gtatgcagaa
    18121 aaccggaggg gtcccccata aaaacttgcc cggtggctaa tattagaaac ccagccaaag
    18181 gtataaataa tagatacagc tcaaagcaat taaaaccgat aaacaaatag ttaagaggaa
    18241 taacccaata taaccaccgc ggaaaatgta cagatataag cgtacaatat tctttcaatg
    18301 ccagaaaact tatcagcgca aaaaacgtca acgtcatcca gcgtggacct gaaatcacca
    18361 gtgaaaaaca aatgacgatg ccccaccagg tacgaatacg taatgtaact tcatcctctt
    18421 ttctctccgg taaccgccat agcagaaaat ggtttatcag cgttgctaaa attaacaacg
    18481 cgaaaagtgt tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact
    18541 ccgacaacga ttaatcgcag tccagagaag cagaattgaa gcaatgctcc ataaaagatt
    18601 attccactgc atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa
    18661 cgcgcgatcg cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc
    18721 gagtaaaccg caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc
    18781 gttactttcc ggtaaaaata aaaatggtaa atagagcgca atatcggaaa taacatcacc
    18841 agtctcattc aaaattgccc caagccgtgt ttgctgattg cactcacgcg ccaacatgcc
    18901 atccagcgca ttgagcgcca tacggataaa aagcacgatg ggcaatagca taaagaggat
    18961 gggttgtgcc accaacatca acagcaatcc ggtaagaaga gaaagcgcca gtgcagcaag
    19021 agtgatgtga ttcgctgtaa cgtggtgctt gtaaagccaa aacatcgtcg gccttaacag
    19081 cgactgaaag agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt
    19141 gcgctatggt gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat
    19201 tcagaatata tcaaggtatt ggggactcac tgtcaggaat aattatcccc aattaatcaa
    19261 agggttaaga aatattacgt tgtggatcaa tacaactcat caagttttct catcgcttgt
    19321 tcccgtattg cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg
    19381 ttaacaagct ctaacgccag caaaggatca cgtttcagcc tcgcctttaa tacatcaact
    19441 ggaaaacggg gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt
    19501 actgcaggcg gtgtgtggcg gttgttaagt gcgccgaact tgaaaaatgt gagattgctt
    19561 ttcgccagtg tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct
    19621 aacgcatcgg ggtcgagtgc ataatagacg cgtaatgctt catcaacctg cgcacgggag
    19681 ctgttttcct gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt attttgctgt
    19741 aattccttga ggacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag
    19801 tcccagtctt ccaaacgtat cagggcggtg gcatttaata gcatctgatc atcaacttca
    19861 ccgtggctga taagctcaga caaaatcata tcagcgctgg tttcatacgc agctgaatct
    19921 gctttcggat tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac
    19981 gtgctcagat actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc
    20041 aataaccgtt gtagagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag
    20101 gcaggggatt ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct
    20161 aaaaataaca cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca
    20221 actttgtcac tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc
    20281 agcttgaacg ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc
    20341 tgattgtagg tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg
    20401 atatagccat caataatgga ttcaggcaat aaccacgcat ctttataaag ctcctgaatg
    20461 atcatatccc aatctctttt cgccagagtc attacgatat ccagagggag attttcacgt
    20521 agcgcaagat tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg
    20581 acagagagcg gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga
    20641 tcggtcaact ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt
    20701 tcatcattgc tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg
    20761 agagttcgca ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg
    20821 ttgccttcat ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac
    20881 agcgcttctt ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta
    20941 aaaatttctt tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt
    21001 attatcatca gcgctttttt gacattttca gtgttatttt tgtcactcgc acggaccatg
    21061 aattcactat ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatattcca
    21121 cgcgcattaa tactcccctt aatcgaagac ggattatcgt aatatgagtc gataaatacc
    21181 agtccacttg catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt
    21241 gccgtcacgt taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct
    21301 ttgactgtca aattaccaag aacaatcaat ttttgattac tctctatatt accttctacc
    21361 actaaatcgc cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc
    21421 acgctactgt tgatagcttg ttcaacttgt tgtttatctt cagcggtagc cgcagaaaaa
    21481 aatggttcaa tatcaactgt atttgcaaca catcgaagtt ccgcctgtac tggaagactc
    21541 agcataaaca ggggaagtaa acctatccag ccgcaaatcc tggacttaaa catatgcact
    21601 ccatgccatt aaaaaagaaa caaaagggaa atgcatcttt cccttttgta tattcgttat
    21661 tctcgcgaaa taccatccaa cgtagacaac acttcctcag aaagatgcaa tttttcagca
    21721 gccatattct cccgtaaatg cgcaacagaa gacgtccctg ggatcagcaa aatattcggt
    21781 gaacgttgta acagccacgc cagcgccacc tgcatgggtg ttgcacccag gctcgcagca
    21841 acatccgaca gcgtagagga ttgcagcggt gtaaagcccc cgagcgggaa gaacggcacg
    21901 taggcaatgc catcgtgggc caaagcatca atcattgcat catcagcacg gtgcgcgatg
    21961 ttgtattcgt tttgcacaca gacaatttcg gcaatcttgc gcgcctctgc aacctgcgtc
    22021 ggtgtgacgt tgctcaggcc aatatgtttt accaggcctt gttgttgcat ctctgccagc
    22081 acggtcaggc tggcctcaat cgatccttcc gctgggccat gaccatctcc catcataacg
    22141 cgcaggttaa ccacatccag tacgtccagg ccgagattac gtagattatc gtgcaccgct
    22201 ttttgcaact ctgccggaga aaaggcgggc aaccaggaag cgtcctctcc acgccgcgca
    22261 ccaattttag tgacaattgt caggtcgtca gagtaaggat aaagcgcttc gcggataatc
    22321 tgattggtga cgtgcggacc ataaaagtcg ctggtatcaa tgtgattgac gcccaatgcc
    22381 agcgcctcgc gcagcacagt tatagcgacg tggcgatctc ttgggggacc aaaaacccca
    22441 ggacctgcca gttgcatcgc gccataacca agacggttaa cggattttgt accgagagta
    22501 aatgtattgc tgctcatcgc accctgccct gtaataaaac ggattaagaa aaatcagtat
    22561 agaaacgaaa agagataaat aatgcgaggg cgtaaacaag attactggat gagttgctct
    22621 attgtcctga aaaacacaaa acaaaacgcg taaagcgccg catccggcaa taaaaaaacc
    22681 gcaacctgta tcagcgttgc ggtttaggta tcacatgcta aaaattagaa tgttcagtat
    22741 ctggacgtta ctgtttcggt cgcagattct catcaaatac cagacgtttc ctgttcggtt
    22801 caacttcccg caatggctca acttgatgca acgtctgctt acaacgagtc accagcacct
    22861 gatactcatg cgtaccctgc tttttccatg ccagctcctg ctcactgagt tcacgaatcg
    22921 ctttcgccgg gctgccgaca atcaggtaat tggcaggcat ttctgctttg gctttcacaa
    22981 atgcggatgc gccaacaatg ctgttctcgc caatcaccgc gccgtccatc actaccgcgt
    23041 tcattcccac taatgcattg cggcggataa tgcaaccatg aaggatagcg ctatgaccaa
    23101 tatgtccatc ttcttccacg acagtatcct gctcgggaaa accgtgcata acgcaattat
    23161 cctgaatgtt cgcgccatct ttcaccacga tacgaccaaa atcgccacgc aggctggcat
    23221 ttggcccaac gtaaacgccc ttgcctagaa taacatcgcc gatcaatacc gctgtcggat
    23281 ggacaaaact ctcttctggc acaaccggag tcagaccatc tatctggtaa attggcatat
    23341 cgcctcctgt tcaatattca aaccgccaaa acgttgaaaa tacaggcttc ccggcgcagg
    23401 cagttcaccg accgaggttt cacctttttc actaacgaac gctaaagcag caggcgctac
    23461 gcgctgataa atgttgatac acagctgacg cgccgtatgc cctgcccagt gcgccggaag
    23521 caactcctct ggcaacaatg ggtctttaag gacgacacgg cgataaaaat ggatcagtaa
    23581 aagctgaata tgaaatgcac gctccggggt taactcgtct gctgcctctt ttaacagcgg
    23641 caccagcggg cggaatgact gaataaaggt ttcgtacatg gcattttgtt cagttaaatg
    23701 ccagcactct tccactctgg cacgcagtgc tgcacgagaa agcgccagtg gtatttgcgc
    23761 ttcaaaacaa atcacgttat ccgccacccc cgcttcatgc aaaagtgtct gtacatccgc
    23821 cagtttttgc gacggcgatg ccatcaggct gggtgccagt gcgccaaaac cttgccagat
    23881 caactgcttt ttgacatcag ccagcgttga tttatctaaa ccttccgaga gcaacaggag
    23941 ccatttacca tcccatgcag gttgctctgc gcgataaatt ttactttctg cccgtcgcgt
    24001 caggcgcaag cctttatcac tgaggctata gaaactgcgt cgcccgatgc gggaaacatc
    24061 cagccagcct tctttattaa gacgaaacaa agcggtgcgc acgaaacgct caccaaatcc
    24121 cagcccttcc agcaaagcag ccagactacc cagccagatt tcaccaccac gatgggaaag
    24181 cgaatcacca tacagagagg agatcaaaga tgtgccactg accggaacag cgtttacagc
    24241 atgttggata aaagtaacaa gtttactcat tcgattcagt ctgttgttat tgttgtgatt
    24301 tatgaatcat agcacaggtc gtatcgactt ctggccctgg cgctttacct caccagggcc
    24361 agacgacacg tcaggcacca acaatattgc gcagatcaaa cacccgacac gccttgcctt
    24421 ctgaacgcgg gatactgcca cagttaacga tcatcacatc ggtagagatc cccaccatcg
    24481 acttaatccg atggcgcagc tggtggcaca cctggcaacg ctgctcatgc gtcaatgtca
    24541 ggctgctttc tttcaactcc actttcacag aaagtgaatc aagatgcccc cggcggttca
    24601 cctccagttg gtaatggggc gataaatgtt cgaatttgac aatctcttct tccagttgtg
    24661 acgggaagac atttacaccg cgaatgatca acatgtcatc gctgcgtccg ctgatgcgat
    24721 ccatgcggcg catagtccgc gccgttcctg gtaacagtcg ggttaaatca cgcgtgcggt
    24781 aacgaatgac cggcaatgct tctttggtca gcgtggtgaa taacagttcg ccatgttcac
    24841 catcggcaag cggtgtgccg tcatgaggat taacaatttc aggatagaaa tgatcttccc
    24901 aaatggttgg gccgtcggta gtttccagac actccatcgc cccccccggc cccatcactt
    24961 ctgacaggcc ataaatatcc agtgcggtga tccccagacg ccgttcaatc tctttacgca
    25021 tggcctgtgt ccacggctca gcaccaaaca ctccaacccg cagcgagcaa ccgctggcat
    25081 caccgcccaa ctgccgctcc agctcttcaa tcaggttaag gcaataagat ggcgtaacca
    25141 tgatcatatc tggttgaaaa tcgcggatca gttgcgcttg tttttccgtc tgcccaccag
    25201 acatcgggat caccgtagcg cccagacgtt cggcaccata gtgcgcgccc agcccaccag
    25261 taaacaggcc gtaaccatag gcaacatgaa ttttgtcttt gggcgtgcca cctgccgcac
    25321 gcaaggaacg cgctacaata ttggcccagt tatcaatatc gttttgcgta tagccgacaa
    25381 ctgtcggttt acctgtggtt cccgaagaag catgaatgcg caccacttgt tccatcggca
    25441 cggcaaaggt gtcgaaggga tagttgtcgc gcagatcctg tttggtggta cagggaaatt
    25501 tacgcagatc tgaaagttcc ctgaaatcat caggatgtac gcctgctgcg tcaaatttgc
    25561 gccgatacat cgggacattt tcatacgcgt gtttgagcgt ccatttcaga cgttgtgttt
    25621 gcaacgcctg taactcatca acagacgcgg tttcaatcgg gtcaagcttt gtattggtta
    25681 tcattgtagg gtactcgcag gttgatatgc tcaaacacgc tccagaatca tggcgatgcc
    25741 ctgaccgaca ccgatgcaca tggtgcacaa tgcgtaacga ccgttacgcc gatgcagctc
    25801 atggctggca gccagtgcca ggcgggcacc actcattccc aacggatggc ctaaggcgat
    25861 agcgcctccg ttggggttaa catgtggggc atcatcaggc agccccaatt cgcgtagtac
    25921 acccaacgcc tgggccgcga acgcttcgtt cagttcaatc acgtccatat cgtgaatact
    25981 cagccccgcg cgttccagca cccggcgagt tgcaggcacc ggaccaagcc ccatcaggcg
    26041 cggttccacc ccggcggttg ccatggctac gattcgcgcc cgcggtgtca gtccttgcgc
    26101 tgctgccatc tgttcactgg caataatcaa cgcagcggct ccgtcattca ccccggaggc
    26161 attgcctgcg gtaatcaccc cattggcacg aaatggtgct tttaacccac gtaactgttc
    26221 cagcgtcgtt tccgggcgca gatgctcatc atgttgtatt tctgttacaa cgcctttctt
    26281 gtttttcaac acaaccggaa caatctcctc agccagaatg cctgaggatt gcgcttttgc
    26341 cgtacgttgc tgactgcgta gcgcaaaact atcttgatct tctcgcgata tttttaacag
    26401 ttcagctaca ttctctgccg tttccggcat gctgtcagtt ccaaattgtt gagccatgag
    26461 cgggttcaca aatcgccagc caatagtggt atcaaacatc tcagcctgac gagaaaatgc
    26521 actggttgcc ttgcccataa caaacggtgc ccgagacatt gactccacgc caccagcgat
    26581 cagcaaatcg ccatcgcccg ctttaatcgc ccgtgcggcg aaccccagtg cgtccagccc
    26641 ggaaccacac aagcggttaa tggttgtgcc ggaaacacta tgcggcagcc ccgccagtaa
    26701 cgtcgccatc cgggctacgt tacggttgtc ttctcccgcc tgattagcac agccgaggat
    26761 cacatcatcg atacactccg catcgagacg cgggtttcgc accagcagct cccgcaaagg
    26821 gatagcagcc agatcgtcag cccgaacacc tgataatgcc ccaccatagc ggccaattgg
    26881 cgtacgaatt ccgtcacaga taaaggcttc acgcatcagg cttctcctgt aatggtgccg
    26941 ccgatgcggt gagatttacc gcgaaacagc gcaaccgttt tttgttgttg gttaacaatt
    27001 tcgatgtcgt aaacaccggt ttgcttgccc tgatgacgta cctgcgcagt agcagttaag
    27061 gtgtctccgg caaagcctgg acgcaaaaaa tcaatcgtgc aggcagaagc gacggctgcc
    27121 agcccctggc tattgcaggc gtaggcaaag gcagtatcag ccagtgaaaa tagctgcccg
    27181 ccgtggcaac tttgatgacc gttaagcatt tgtgcagtga cggtcatggt cactacagca
    27241 aagccttcat ccattgagat aatgtcgatg ccaagcgctt tggcgcaggc atcgttctca
    27301 tacattgcat gggcattttg ccaggcctta tgactcataa ccgctctcca gaagcgctcg
    27361 ttggcgcagc aatgaacatg ggcgatagcg ttcttcgcca tagtgatgtt gtagattttc
    27421 caggagcctt aatattcgct gccagccaag ttgcgctccc caggcaagcg ggccatatgg
    27481 ataattcacc ccaagacgca tggcggtatc gatatcctgt tcagaggcca cgcctttttg
    27541 cagcgcatca agggcttcat tgatgatcat tgctaccgtt cgccaaatca gcattcctgg
    27601 gtaatctgca atttgcagca ctgttttgcc ctgctgttgc aggtaataaa tggccttgcg
    27661 ggtcgctgag tccgggttca ccgctgccgc agcaatggtc accaccttgc ccgccatttt
    27721 gtcgaccact accaccgggc gtgccagtcg tattgccagc gcctgtgccg tctcgccttg
    27781 tgtctcaatc aataaaacat cgtcaatttc cgtgacgccg tcacttttct tttctacttt
    27841 cattgggcta aaactgtcgc ttaccgcttc caggccaaca actgcctcac gttccgcgcg
    27901 ccagtcgtac acgcccagcc cacttttctt gcccaaccgt ccaccaatca ccagttcctg
    27961 ttgcaccagc gaaggtaaaa aacgacgctc ctgccagaaa gcgttaaaca ccgaacaggt
    28021 gacagcaaaa ttgacgtcct gaccaatcag atcggttaat tccagcggcc ccatcgggaa
    28081 accagcgcca tcgcgaagtg cagcgtcaat cacttctggt gcagcaacct gctcttccag
    28141 tgcccgccag gcctcggaat aataaggacg cgccacacgg ttaacgataa atccaggggt
    28201 cgaatgacag cgcacaggct gcttacccca actcaacgtt agttcacaca actgctcaac
    28261 aacttccgct gccgttgcca gcccactgac cacctccacc aacttcatca ccggtgccgg
    28321 gttaaaaaaa tgcagccccg caacacgttc aggatttttt acctccgcag caatcgcggt
    28381 tatagagatt gacgaagtgt tagtggtcaa tagcgtttgt ggcgggcaaa cttccgccag
    28441 ctgtgcaaag agcgcttttt tgacttccag acgttcagac gccgcttcaa tgaccaggtc
    28501 cgcagctgcc agcgcgtgaa tatcggtcac cgggatcagg cgtttcaatg tgcgttcaca
    28561 ggtttcagca gtcagttttc cccgcgtcac gcgtgaattt agccgcgcgt gtatcccgtc
    28621 gattgcgcgg gtcagcgctt cagcagaaat gtcatacagt aaaacctgat gtccatgact
    28681 ggcggcaact tcagcaatgc cagcccccat ggtgccgctc ccaatcactg ccacagtttg
    28741 cacatttatc atcatgctat ttccccgtga actgcggtga gcgtttagcc aggaacgcac
    28801 tgacaccttc acgataatct gcgctccgtc cggcaagtcg ctgatagtcg cgttccagat
    28861 ccagttgcgt atcgagcgta ttggtttcag cgctatttat cgcttgcttg ataagtccaa
    28921 gaccaaatgt cggttgtgtc gccagatgcc gtgccagctg ttgcgcggta tctgccagcg
    28981 tttcatcatc aacaacctgc cagatcatcc cccattcgtg cgcctgttca gcactcagtt
    29041 gattccccag cagtgccagc cccatggcgc gcgctcgccc agcaacgcgt ggcagtaacc
    29101 aggttccacc gcaatcgggt attaagccta acttactaaa cgccatgacg aattttgctg
    29161 aacgggcagc aataacgatg tcgcacccca gtgccagtgt tgcgcctgcc cctgccgcca
    29221 cgccattgac tgcacagatc accggttttg gcagttttgc caggcgacgt accagtgggt
    29281 tatagaaacg ttcaactgac atccctaaat ccggtgcggg gcctgtggga tcaacgttac
    29341 gatcgttaag atcctgacca gcacaaaatc cgcgcccggc acctgtaagt aacaggcaac
    29401 ggatagtgtc gtcgcgctcg acctgtttca ggcactctgc cagttgtgcg tgcatctcat
    29461 cattaaaact gttcaggcgt tccgggcggt tgagcgttag tgtcatcacg cccttttcta
    29521 catgactgag gatgaattcc atcattagcg tcctttaaag tcgggcgtgc gtttttgtaa
    29581 gaaagcggag atgccttcat gacgatcttc tgttgccgcc agcaaggtga ataactgtcg
    29641 ctcctgggca agtccggctt gcaaagccac ttcctgcgac tggcgcagcg cttgctttgc
    29701 cgcttgtaag gccaacggcg agtgacgtgc cattttcgat gccagctgta aggcgtattc
    29761 gagggttaaa tcgctgggga agacgtcgct aaccagcccg gcctgctgtg cttgctgggc
    29821 ggtgatactt tctccgctca gcaccatttt gctggctaac gatttaccga cactacggat
    29881 taaacgttgc gttccgcctg cgccaggcat gatgccgaga gtgatttccg gcaacccaaa
    29941 acgcgcgttc tctccggcaa ccaccacatc gcacaacaat gccagttcgc aacccgcccc
    30001 aagcgcgtaa ccattgacgg ctgcgataag tggtttatta aaggcctgca atcgcgccca
    30061 tagctgcgga cgtgtatcgt ttaaggtggc cgcgagatct ttttctgcca tttcgttgag
    30121 atcggcccca gcggcaaaaa agcgtgcatt accggtaatc acacagaccg aaatgctggt
    30181 atcggtagcc gcagcttcca gttcatttac cagttgcatc agcagggcat tatttagcgc
    30241 attacgtgcg gcgggacggt taagggtcag caacaatact cgttgctgac ggctgacgat
    30301 cagttcgctc atgccatccc cttcgcgtca aagtcaacca ccacatcgct ggtcagcggc
    30361 agtgcctggc aactcaacac ataacctgcg gccagttcat ccggttccag actgtaattg
    30421 gtttccatcg ccactttgcc acgcagcact ttgcatttgc aggtcgcaca gacgccgcct
    30481 ttgcaggcat agggcagatc agccccctgg cgcaatgccg catcgagaat gctttcatcg
    30541 tcggcattaa gcacgatttc ccgatcccgc ccatcctgac gtacagtcac tttttgtccg
    30601 tcactttgca cgttaacgct acgtttgacg cgcgtgccag gcgtattaaa ccgctccaga
    30661 tgaatggttt tatctggcat tcccagtgct tttaaggcgg cttccgtctc atccatcatc
    30721 gccgccggac cacaaataaa tgcctcatca taaagacgaa aattaatgag cgaggcccca
    30781 agtgactgta atctttcacc gtcaatacgc ccgtgaagca gatcgctgtc gagggtttcc
    30841 tgactgaaaa tgcacaacaa ctgtaaacgc tgaggatatt tgtctttcag gtctgccagt
    30901 gcctggcgaa acatcatgct ctggctggta cggttaccgt agatcagggt gaactgactt
    30961 tcaggctcgg tttgtaaagt ggtggcgata atcgccagca ttggcgtaat acctgatcct
    31021 gctgcaatag ccagatagcg gccctggcgt tcggcctggg gctgatagcc gaaatgcccc
    31081 tgcggcacca tgacctccag cgtcataccc tggcggatgt gttcgcgggc atagcgggag
    31141 aaacgtccgc cttcaatggc tttcaccgcc acactaattt cgccaggcag gtagctgcgg
    31201 caaatggagt aacaacggcg taattcttca ccatcaagac tggcttttaa ggtcaaatgt
    31261 tgaccggggc gaaagcgata cgcctcctgc aaaggctggg gcaccgcaaa ggtaatggtc
    31321 accgcatcac gggtttccgg ctcgactttt gccactgtta aggaatgaaa cgttgtcatg
    31381 gcatcctcaa atacatttga aataatcgaa aggttcgcgg caactatcgc agcggtacaa
    31441 tgctttgcag gccgtggaac caaattcact gataagtgtg gtatggacgc tggcgcagcg
    31501 cgggcaacgt acttctggcg gcaaatgggc atggcaactg tgtccggcgg gcgggctaat
    31561 gccatactgt cgcagacgct cacgggcatc gggggtcatc cagtcggtgg tccatgccgg
    31621 gtcgagttgc agcacaacct gaacgggggt aaacccgtgg gttgtcattg cctcacgtat
    31681 cgcgccaatc aaatgttccg ttgccggaca accggaataa gtcggcgtaa agccgatcac
    31741 ccatccttct cccatctgtg tcacattacg caccatgcct aaatcggtaa tggtcagcac
    31801 cgggatctcc gggtccggga tctggctgag tagcgcccat atctcatgaa cctgtggcgg
    31861 tgcaatggtg gccagacgtt gcatacccat ctcctctgtt accattgctg accgggcaag
    31921 acacgctgga gatactgcat ttctgccagc atgggtccaa ggtgttcggt atgcagtcct
    31981 tttttaccgc cagtgcgata cgcctgctct tgcggtacgt tcaatgtggc ttcgttgatt
    32041 ccggcaaaaa cttcggcttc ccacgctgcg cgtaaagtgc gtggatcaac cgcaataccc
    32101 tcttcactca gtgcaatatc aatctcgtcg gcatcgaaca gttcggcagt aaaacgccac
    32161 aacttgtcga tggcctgctg catcttttgc cctgatacgt caataccatt gcccagtcgc
    32221 tccagccagc cacgactaaa acgcaggtga taacgcgctt ctttaattgc tttggcagaa
    32281 atcgccgcca gttgcggatc acggctttcc atcagacggg taaagagcgc cacatgccat
    32341 gcatcgatga aatactggcg tgcaatggtg tcggcaaaat tgccgtttgg ttgttcaacc
    32401 aacaataagt tgctgaactg gcgctcgtct cgggtgaagg ccagggtatc ttcatcacct
    32461 tctcccgcta attcagcggc atacgataag aagttacgtg cctgacctaa taaatcgagg
    32521 ccaatgtttg ccagtgcgag atcgatttcc agttccggtg cgtgaccgca ccattccccc
    32581 aaccgctggg agagcaccag atagttatcg cccaggcgca aggtgtaagc cgttaactga
    32641 ttcatttccg acctcacatg tgctcaatgc catcagggat ggtgtaaaac gttggatggc
    32701 gatagacctt gctttcagcc ggatcaaaaa attcaccgcg ttcttccggt tgcgaggcaa
    32761 caatttcact cgccttcacc acccaaattg aacatccttc gctacgacgg gtgtaagcat
    32821 cacgggcatt ttccagtgcc atccgctcat cggcagcatg taaactgcca acatggcggt
    32881 gtgataagcc ctgtttgcca cgcacgaaaa cttcgtataa cggccagtaa acattactca
    32941 ttttgaatct ccttatgcga ccttacgggc aagttgtttt tgtgcatggg ccagcgctgc
    33001 ttcccgtacc caggtacctt cttcccaggc tttacgtttg gcgtcgagcc tctcctgatt
    33061 acaaattccg cgaccgttaa ttacttcgtt aaactcctgc cagtcgatct caccaaagcg
    33121 gtagtgaccg ctttcagtgt caaaatgcag atccgggtca ggaacggtca taccgagcat
    33181 ttcaacctgt ggaacggtgt tgtccacgaa acgctggcgg agttcgtcgt tggtgaaacg
    33241 tttgattttc caggcgagac ttctggcgct gtttggcgag ttatcatcgt ttggcccgaa
    33301 catcattaag gctggccacc agaaacggtt aatggcgtct tgcaacatct gcttttgagc
    33361 ttcgctgccc tgcgccagtg ccatgcaggc ttcaaacccc tgacgctggt gaaaactctc
    33421 ttctttgcag attttcacca ttgctctggc atacgggcca taagaagttc ggcataacgc
    33481 cacctgattg acgatcgctg cgccatccac cagccagccg ataacgccga tatcagccca
    33541 actcaatgtc ggataattaa agatggagga atatttcatc cgtccgtcga gcattttttg
    33601 gtagatgtct tctctggcac agcccagtgt ttcggcggcg ctatagagat acagaccatg
    33661 accggcttca tcctgcactt tggctaacag aatggcttta cgccgcaagg ttggtgcgcg
    33721 agtgatccag ttaccttcag gcaacatacc aacaatctcg gagtgcgcat gctgcccaat
    33781 ctggcggatc aatgtctttc ggtaagcatc gggcatccag tcctgcggct cgatagccgt
    33841 ttcctgggct atccgttgct caaagcgttc ttcttgggtc acgtcatcac ctttacgatt
    33901 ccagttttcg tgaattaatt aaaaacacga atcacgttgt tttatgaaag ttacacaatg
    33961 gttaactaaa gtcacaaatt ttgtatactt gaactgtgac agatttcgca ctctattttg
    34021 ataactcatt atttatcagt gcattaatgt ttttagtagt tgatatacag atcacgcagt
    34081 taacatttcg ttaaaagatc ccttgctttt tatgattcgc gatttaacta ttagcaacag
    34141 aaatgtgaaa catctggaga gtagcgatgc agcagttagc cagtttctta tccggtacct
    34201 ggcagtctgg ccggggccgt agccgtttga ttcaccacgc tattagcggc gaggcgttat
    34261 gggaagtgac cagtgaaggt cttgatatgg cggctgcccg ccagtttgcc attgaaaaag
    34321 gtgcccccgc cctccgcgcg atgaccttta tcgaacgtgc ggcgatgctt aaagcggtcg
    34381 ctaaacatct gctgagtgaa aaagagcgtt tctatgctct ttctgcgcaa acaggcgcaa
    34441 cgcgggcaga cagttgggtt gatattgaag gtggcattgg gacgttattt acttacgcca
    34501 gcctcggtag ccgggagctg cctgacgata cgctgtggcc ggaagatgaa ttgatcccct
    34561 tatcgaaaga aggtggattt gccgcgcgcc atgtactgac ctcgaagtca ggcgtggcag
    34621 tgcatattaa cgcctttaac ttcccctgct ggggaatgct ggaaaagctg gcaccaacgt
    34681 ggctgggcgg aatgccagcc atcatcaaac cagctaccgc gacggcccaa ctgactcagg
    34741 cgatggtgaa atcaattgtc gatagtggtc ttgttcccga aggcgcaatt agtctgatct
    34801 gcggtagtgc tggcgacttg ttggatcatc tggacagcca ggatgtggtg actttcacgg
    34861 ggtcagcggc gaccggacag atgctgcgag ttcagccaaa tatcgtcgcc aaatctatcc
    34921 ccttcactat ggaagctgat tccctgaact gctgcgtact gggcgaagat gtcaccccgg
    34981 atcaaccgga gtttgcgctg tttattcgtg aagttgtgcg tgagatgacc acaaaagccg
    35041 ggcaaaaatg tacggcaatc cggcggatta ttgtgccgca ggcattggtt aatgctgtca
    35101 gtgatgctct ggttgcgcga ttacagaaag tcgtggtcgg tgatcctgct caggaaggcg
    35161 tgaaaatggg cgcactggta aatgctgagc agcgtgccga tgtgcaggaa aaagtgaaca
    35221 tattgctggc tgcaggatgc gagattcgcc tcggtggtca ggcggattta tctgctgcgg
    35281 gtgccttctt cccgccaacc ttattgtact gtccgcagcc ggatgaaaca ccggcggtac
    35341 atgcaacaga agcctttggc cctgtcgcaa cgctaatgcc agcacaaaac cagcaacatg
    35401 ctctgcaact ggcttgtgca ggcggcggta gccttgcggg aacgctggtg acggctgatc
    35461 cgcaaattgc gcgtcagttt attgccgacg cggcacgtac gcatgggcga attcagatcc
    35521 tcaatgaaga gtcggcaaaa gaatccaccg ggcatggctc cccactgcca caactggtac
    35581 atggtgggcc tggtcgcgca ggaggcggtg aagaattagg tggtttacga gcggtgaaac
    35641 attacatgca gcgaaccgct gttcagggta gtccgacgat gcttgcagct atcagtaaac
    35701 agtgggtgcg cggtgcgaaa gtcgaagaag atcgtattca tccgttccgc aaatattttg
    35761 aggagctaca accaggcgac agcctgttga ctccccgccg cacaatgaca gaggccgata
    35821 ttgttaactt tgcttgcctc agcggcgatc atttctatgc acatatggat aagattgctg
    35881 ctgccgaatc tattttcggt gagcgggtgg tgcatgggta ttttgtgctt tctgcggctg
    35941 cgggtctgtt tgtcgatgcc ggtgttggtc cggtcattgc taactacggg ctggaaagct
    36001 tgcgttttat cgaacccgta aagccaggcg ataccatcca ggtgcgtctc acctgtaagc
    36061 gcaagacgct gaaaaaacag cgtagcgcag aagaaaaacc aacaggtgtg gtggaatggg
    36121 cggtagaggt attcaatcag catcaaaccc cggtggcgct gtattcaatt ctgacgctgg
    36181 tggccaggca gcacggtgat tttgtcgatt aatcggtgaa tgaagggcaa cggccaatag
    36241 ttgccctttt atttcactaa gttttgtgac gttgtcacat tatgcatgat gtgtacatct
    36301 attttcaggg cacccactgt atgaaaagct ggcacacctg ccaaacaacc tggcaggtgc
    36361 aggcaatccc ctttgcatca gtactgataa tgtgaacctg actaaaccgc ccacagagcg
    36421 cggttgctaa caagaacaca acatctgacg aggttaataa tgggaagccc ctctctgtat
    36481 tctgcccgta aaacaaccct ggcgttggca gtcgccttaa gtttcgcctg gcaagcgccg
    36541 gtatttgccc acggtggtga agcgcatatg gtgccaatgg atgaaacgct taaagaattt
    36601 ggtgccgatg tgcagtggga cgactacgcc cagatcttta ccctgattaa agatggcgcg
    36661 tacgtgaaag tgaagcctgg tgcgcaaaca gcaattgtta atggtcaacc tctggcactg
    36721 caagtgccgg tagtgatgaa agacaataag gcctgggttt ctgacacctt tattaacgat
    36781 gttttccagt ccggtctgga tcaaaccttt caggtagaaa agcgccctca cccacttaat
    36841 gcgctaactg cggacgaaat taaacaggcc gttgaaattg ttaaagcttc cgcggacttc
    36901 aaacccaata cccgttttac tgagatctcc ctgctaccgc cagataaaga agctgtctgg
    36961 gcgtttgcgc tggaaaacaa accggttgac cagccacgca aagccgacgt cattatgctc
    37021 gacggcaaac atatcatcga agcggtggtg gatctgcaaa acaacaaact gctctcctgg
    37081 caacccatta aagacgccca cggtatggtg ttgctggatg atttcgccag tgtgcagaac
    37141 attattaaca acagtgaaga atttgccgct gccgtgaaga aacgcggtat tactgatgcg
    37201 aaaaaagtga ttaccacgcc gctgaccgta ggttatttcg atggtaaaga tggcctgaaa
    37261 caagatgccc ggttgctcaa agtcatcagc tatcttgatg tcggtgatgg caactactgg
    37321 gcacatccca tcgaaaacct ggtggcggtc gttgatttag aacagaaaaa aatcgttaag
    37381 attgaagaag gtccggtagt tccggtgcca atgaccgcac gcccatttga tggccgtgac
    37441 cgcgttgctc cggcagttaa gcctatgcaa atcattgagc ctgaaggtaa aaattacacc
    37501 attactggcg atatgattca ctggcggaac tgggattttc acctcagcat gaactctcgc
    37561 gtcgggccga tgatctccac cgtgacttat aacgacaatg gcaccaaacg caaagtcatg
    37621 tacgaaggtt ctctcggcgg catgattgtg ccttacggtg atcctgatat tggctggtac
    37681 tttaaagcgt atctggactc tggtgactac ggtatgggca cgctaacctc accaattgct
    37741 cgtggtaaag atgccccgtc taacgcagtg ctccttaatg aaaccatcgc cgactacact
    37801 ggcgtgccga tggagatccc tcgcgctatc gcggtatttg aacgttatgc cgggcctgag
    37861 tataagcatc aggaaatggg ccagcccaac gtcagtaccg aacgccggga gttagtggtg
    37921 cgctggatca gtacagtggg taactatgac tacatttttg actggatctt ccatgaaaac
    37981 ggcactattg gcattgatgc cggtgctacg ggcatcgaag cggtgaaagg tgttaaagcg
    38041 aaaaccatgc acgatgagac ggcgaaagat gacacgcgct acggcacgct tatcgatcac
    38101 aatatcgtgg gtactacaca ccaacatatt tataatttcc gcctcgatct ggatgtagat
    38161 ggcgagaata acagcctggt ggcgatggac ccagtggtaa aaccgaatac tgccggtggc
    38221 ccacgcacca gtaccatgca agttaatcag tacaacatcg gcaatgaaca ggatgccgcg
    38281 cagaagtttg atccgggcac gattcgtctg ttgagtaacc cgaataaaga gaaccgcatg
    38341 ggcaatccag tttcctatca aattattcct tatgcaggtg gtactcaccc ggtagcaaaa
    38401 ggtgcccagt tcgcgccgga cgagtggatc tatcatcgtt taagctttat ggacaagcag
    38461 ctttgggtaa cgcgttatca tcctggcgag cgtttcccgg aaggcaaata tccgaaccgt
    38521 tctactcatg acaccggtct tggacaatac agtaaagata acgagtcgct ggacaacacc
    38581 gacgccgttg tctggatgac caccggcacc acacatgtgg cccgcgccga agagtggccg
    38641 attatgccga ccgaatgggt acatactctg cttaaaccat ggaacttctt tgacgaaacg
    38701 ccaacgctag gggcgctgaa gaaagataag tgattgtttc agacaaaaaa acggcaccag
    38761 gtgccgtttt ttacttatga gcgaaccaga ttaataccgt acacacaccg acttagtttc
    38821 acaccaaccg tccagccagt cggggccaaa atcacggccc gttcctgact gcttcatccc
    38881 accaaacggt aagttagcgt caattaaggt atggctgttt acccacaccg tccctgcctg
    38941 taagcgatcg ctatattcca gagcctggga gagattttgc gtccagacac tggcagttaa
    39001 gccatattcc gtgtcgtttg ccagttgtaa cgcctcttct ccatccgcta ctcgcaccag
    39061 gtttaccacc ggaccaaaca cctcttcacg agttaagcgc aatttagcat cgggatttac
    39121 caccagcgtt ggcgcaacat aatacccctc tccggctggt ccattcgacc cgcgaatcag
    39181 ctctgcttgc tgtgcctgcg catcgtcgag gaatgaacac actttgtcgc agtgcgcacg
    39241 agaaaccaaa gggttaatct gtgcaacagg tgacatcccc ggtcccactt gcaacgattt
    39301 taccgcctgc tcaaatccac taaccagcgt gtcaaacaac ggcgcttcaa tataaattcg
    39361 cgaactggcg gcgcatactt gcccttgatt caggaagctt ccggtcatca agccttcaat
    39421 aacccattgc ggatcagcat cttttaatac aattgccggg tttttaccgc ccagttccag
    39481 cgttacacgc gttaagtgat cagcagcagt tctggcaata ccttttcccg tcgcggttga
    39541 accggtaaaa ctgattttcg caacatgagg atgtgacgtc agggccgcgc cgcatacagc
    39601 acctgacccg gtgacgacat taaaaacgcc atcagggata ccagcctcgc tggccagttc
    39661 cgccacgcgc aacatcgtca gtggcgtggt ttccgaaggc ttaatcacga ttgaacagcc
    39721 tgctgccagt gctggcatca ccttccacat accaatcatc aacggaaagt tccatggcac
    39781 aattcccgcc actacgccaa ccggctcttt acgcgtccag gcctgataac gcgccccctg
    39841 gggtaaggga atcgacaagt ccagcgtttt acccgcgatt ttggtcgtta acccggcggt
    39901 ataacgcatc cagttcagcg tacagcccac ttcaaaagca cgagaaatgg caattgactt
    39961 gccttgctcc agggtttcca gttgcgccag ctcctcactg tgctgctcca ccagatcagc
    40021 aaaacgtagc agaatacgtt cacgctctgc gggtaatcgc ccggcccagc gacgcgagac
    40081 aaaggcccgc caggcagaca tgactgcgtt atctacatcc gcttcgttgg catcagcagt
    40141 agacgcaatt tcttgcccgg tggccggatc aaagatcgcc aatcgttttt cactttgtgc
    40201 ggggcctgga cgaccatcaa tataaagacc gtgttgacga tcgagaaact gttggacctg
    40261 gcttaatact gctacatgcg gctctgtcat cacttttcct tattatttac ccagtgtgat
    40321 ttcagtgtac gcagcgacaa gaaaagtcac ttttttcgct gtgacattcg ctttgtcagc
    40381 tgtgacaagc tccgcaaatc gtgacaataa ccgcttatgt ctctatgcct gagaagactt
    40441 atttcctata gtcaggctca ttagcttcgt ttactgcaac acaaatgcaa caataaaaat
    40501 acatttcaca gagcgaaaac gtgccatgaa ccccgcagtg gataatgagt ttcagcaatg
    40561 gctttcccaa atcaatcagg tatgcggaaa ttttaccgga cacctgctga ctgagcgtta
    40621 cacgggtgta ctggatacgc attttgccaa aggactaaag ctgagtaccg tgacaaccag
    40681 cggggtgaat ttatcccgca cctggcagga agtaaaaggc agcgacgatg cctggtttta
    40741 caccgttttt cagcttagtg gtcaggcaat aatggagcag gatgagcgtc aggtgcagat
    40801 tggcgctggc gatattacgt tactcgatgc ctcacgcccc tgttcgcttt actggcagga
    40861 gtcttctaaa cagatttcat tacttttgcc acgcactctg ctggaacaat attttcccca
    40921 tcaaaaacct gtctgcgcag aaagactgga cgctgactta cccatggtgc aactcagtca
    40981 tcgcctgtta caggagagca tgaataatcc ggcactttct gaaacagaaa gtgaagctgc
    41041 gctacaggcg atggtgtgtc tgctgcgccc ggtacttcat cagcgggaat ctgttcaacc
    41101 tcgtcgtgaa cgtcagtttc aaaaagtggt tacgttgata gacgataata ttcgcgaaga
    41161 gatattacgc ccggagtgga tagccggaga gacaggtatg tcagtacgta gtttgtaccg
    41221 aatgtttgcc gataaaggtt tggtagtcgc gcaatatatt cgtaaccgtc gtctcgattt
    41281 ttgtgcagat gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca tcggctttca
    41341 ttggggattt tctgaccaga gtcatttttc aacggtattt aagcaacgct ttgggatgac
    41401 gccaggcgag tatcgacgta aattccgcta actaattggc gttgtgtgta ctgcaacgcc
    41461 aattagttaa aacagatatt tgactatgca tttttcaggg cgaaaggcaa tttgacagga
    41521 gtttttgaat attcaccttg tttcaggaaa atagatcaat cgctttctcc tgtagcgtta
    41581 tcccgatatt gcggttttgc tattaaaatt ttcgcaccca tttaccgttt atcccttgta
    41641 catattcccc cggtttggcc cgagctacga gcttttgccc ggcgagtttc gcgatatcat
    41701 ctacagacac attattttgc tttgccagtt gttggtaact cgctttacgt tcggcattaa
    41761 tgtcggttac taatttctct gtttcagcat ccgttttcag cgcaaccaga taaccataaa
    41821 atgtttcacc tacccgcccc tgagttctgg cttcatccag agtcaatgcc attacattgc
    41881 tgcttaccag cccaacaaga aacgcacaaa aaagtaatgt tttcttcatc atcgcctcag
    41941 aaaagatcgc tacgagtttc aagcagctct tcgacatctt tgtctgcctt gatgatgatc
    42001 tcatgctcaa ttttaacgtt catattgata gtgatcggtt ccttgggcgc agcgacttca
    42061 atacgcggcg tacaggcagc cagcataaaa gatgacgtca acgcagccag taaaattttc
    42121 attgtttttc ctcacactct tttccttgcg gacagtcatt tcccggcaga cgtgcgttct
    42181 gctccaacca tgcctggaga ttatcgccaa agcgtaaact gcgccacagc gtaaaaatat
    42241 tctcttcatg atggtaatta aggtttaccg taccgctttt accatcaacg cgactggtac
    42301 ctgtaatgtt ggcctgcatg gttaataaac cgagattatc taagttaatt tttgtcgatg
    42361 accggctaat ttccatatag cgcaaccagt taattgctga acccgcagtc atattgtctt
    42421 tcaccaccgc atctgcggtg tctttatcca gccgcaaggt cattggcccg ctatttgcca
    42481 gccaaccatc tttcactatc catttttcgc tgtttagcca taacggcaat gcaccactaa
    42541 atgccccgga catggcgaat tgtttcggat tgacggcgct aaccagttcg ctggatgaaa
    42601 gattattcag ccgcaacagc gccgggtcat gttgcggcat acgtaattgt ttcatcagta
    42661 cgttaccgcc taacacatcg acgctaacat cagtcaacag caagggttct tcttcagtcc
    42721 acggataacg cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga ttaatcactt
    42781 cggcaattcg caacgtaacg gggccgcgag tccccagatg ccaggctcca tcggcaaaac
    42841 ggaaaggcag gacaaaatcg acaccgttaa cctgattatc tggcatccag gcactaccgc
    42901 ctttcaacac gccgtgtcct cccgcgcgga atccttgttc aggcgcagca gaaaatgcaa
    42961 cctgagcata cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc accagcggct
    43021 ggaatacagt cagtgattgt tttggccacc aggcgttacc gcgcagacga ataccgtccc
    43081 agcgaccatt tacccgaacc gggccaatct caccagcatg taaatcgcct ttaaagagga
    43141 aatacgtcgg atcgcgccca tcgacgctaa attttaaggt tgatggcggt aacacactgc
    43201 cgccagtgaa cagcgtttgc ccggcgtcca gtgacagcgc gccgctgaag gagggatgct
    43261 gtgcgtcacg tacccagacg acgggcttgt cgagaattaa tcgcggcttt tctaccgtca
    43321 tcgtaccgta ttgtaactgg tcgaaaccgg tggagaggtc ggtcagcgta atcgtgctgt
    43381 catgccattc accttttcct gcgacatccc aggtggcatt catcggtgta aaactgcctt
    43441 ttccccagta gcgccactgc cagcggcccg cgtcagggag aaaatcattc gccagcccat
    43501 ccatatgcag cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg gcctgcaaac
    43561 gtccgtcaac accacgttgg gtgactttta cacccgctaa aggccagcgg atttcatcga
    43621 tgtccagcga atcgatgact cttcccttcg aacgtaataa cgcgcctggc tcaaaggcca
    43681 gcgttgggtc agtcagactt ccacttagct gcgcgggtaa gcgggcatat aaaatgaggt
    43741 ccgcctgttt cgcttcaccg gtcagttgca gaggcagctg gctgttatcc atgcttaatt
    43801 ttcctgggcc aaaatttagc accgcgttgc ctttacccgc ttgcccctgg gtcagcacac
    43861 tcagtcgtcc gctgatcaga gcgttctcaa gccctgcctg ccagttgtcg actttgacac
    43921 ccagtcggcc actcaaagga aaacctgcat acggccaact ccagcgacca tcgcttacgg
    43981 tcaattgttg acgagtaatt tgccacggca aatcgagcaa cggatcgccg ttatcccgtg
    44041 ccagcacaat caattgcccg ctattttcct gccagtccag ctctgcatcc accagtgacg
    44101 gttcctgcgg caagtttaac gtcgcagtag catgcccact tacaggaagt ccatccggca
    44161 cgagcggcat agtaaattcc cccaccagtt taaccggcgg ctgattttca aacgcgacga
    44221 catccagttc gctgactgta agttgttgcc ctttcagctg gccttgaaat ttaacttttt
    44281 cgccctgata acgcagttgc tggatatcag aggttaatgc gagagagagt tttccctgcc
    44341 attcctgcca gggagaaaaa atcagtttat cgatattgat ccaggtgtta ggcagcatgg
    44401 actgccactg cgcgagggtt tttggagcag ctggcgattg ctccgtctgc ggcaatttcg
    44461 ccagacaagc agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg ctgggatgtg
    44521 aaaggctggc gttggtgata tgcgcaagct gacaatctcc caccagataa cggagatcgg
    44581 gaatgattaa acctttacgc gtaatgcgtg ggctttcatc taatgcaata cgtgtcccga
    44641 gcggtagcca gatgcccgcc agagtgggaa cccacagccc gagcgtcatc aacagcgtca
    44701 acggcacaag aataatcagt aataacagcg cgagaacggc tttatattta cccagcatgg
    44761 gtagttaata tcctgattta gcgaaaaatt aagcattcaa tacgggtatt gtggcatgtt
    44821 taaccgttca gttgaaggtt gcgcctacac taagcatagt tgttgatgaa tttttcaata
    44881 tcgccatagc tttcaattaa atttgaaatt ttgtaaaata tttttagtag cttaaatgtg
    44941 attcaacatc actggagaaa gtcttatgaa actcgccgtt tatagcacaa aacagtacga
    45001 caagaagtac ctgcaacagg tgaacgagtc ctttggcttt gagctggaat tttttgactt
    45061 tctgctgacg gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat gtattttcgt
    45121 aaacgatgac ggcagccgcc cggtgctgga agagctgaaa aagcacggcg ttaaatatat
    45181 cgccctgcgc tgtgccggtt tcaataacgt cgaccttgac gcggcaaaag aactggggct
    45241 gaaagtagtc cgtgttccag cctatgatcc agaggccgtt gctgaacacg ccatcggtat
    45301 gatgatgacg ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg acgctaactt
    45361 ctctctggaa ggtctgaccg gatttactat gtatggcaaa acggcaggcg ttatcggtac
    45421 cggtaaaatc ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc gtctgctggc
    45481 gttcgatccg tatccaagtg cagcggcgct ggaactcggt gtggagtatg tcgatctgcc
    45541 aaccctgttc tctgaatcag acgttatctc tctgcactgc ccgctgacac cggaaaacta
    45601 ccatctgttg aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac
    45661 cagtcgcggt gcattgattg attctcaggc agcaattgaa gcgctgaaaa atcagaaaat
    45721 tggttcgttg ggtatggacg tgtatgagaa cgaacgcgat ctgttctttg aagacaaatc
    45781 caacgacgtg atccaggatg acgtgttccg tcgtctgtct gcctgccaca acgtgctgtt
    45841 taccgggcac caggcattcc tgacagcaga agctctgacc agtatttctc agactacgct
    45901 gcaaaactta agcaatctgg aaaaaggcga aacctgcccg aacgaactgg tttaatcttg
    45961 ccgctcccct gcaatccagg ggagctgatt cagataatcc ccaatgacct ttcattctct
    46021 attcttaaaa tagtcctgag tcagaaactg taattgagaa ccacaatgaa gaaactagcc
    46081 gcgtttgttg cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa aattgctgtt
    46141 acgccagaac agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg taagcccgtg
    46201 accagcgata aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat ttccggcagc
    46261 atgtgtaacc gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac agccaaaggg
    46321 ctggcaatga cccgtatgat gtgcgctaac ccgcagctta atgaactcga taacaccatt
    46381 agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt aacgctggcg
    46441 accgcgaaac agacattaac ttataagctg gcggatttaa tgaattaata gctgccacag
    46501 ctcccggcgg caagtgactg ttcgctacag cgtttgccgt tgggtaatgc acacatccca
    46561 atcgccgtac catccagttg acgggcaaca gaaagcgaac cgccgatcat tgcacaattt
    46621 gcttctccac tactggacat cgacgctttt aaacctggcg ctacgtgcgc cgcagtcgcc
    46681 tgctgaacag gttcactact acacgccgac aacaataaag cggcacaccc tacccaaaac
    46741 gctgctcgca tctcttcttc ctctgatctt caagccaaac gacaccgcca taaataatag
    46801 gcagcacaga gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt ttactttttt
    46861 atggctattt ttttgccctc tgtttgatca aaacattcat tacgctgatg tgggggacac
    46921 aaaagcgaaa atgcagaaga aagccatttg ctaaaattga aggattacta ctgggcgcgc
    46981 agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt actattgacg
    47041 gtaatggcgc ggttgcttcg gtggcgtttc gcaccagtga agttatcgcc atctacccta
    47101 ttacccccag ttccacgatg gcagaacagg ctgatgcctg ggccggaaac ggcttgaaga
    47161 acgtttgggg agacacacca cgcgtggttg aaatgcagtc ggaagcgggt gctatcgcta
    47221 ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc gtttacgtca tcgcagggtt
    47281 tgctgctgat gatcccgacg ctgtacaaac tggcaggcga actcacaccg tttgtcctgc
    47341 atgtagcggc acgtaccgtt gccacacatg cactctctat ttttggcgat cattccgacg
    47401 tcatggcggt gcgccagacg ggttgcgcga tgttgtgtgc ggcaaacgtc caggaagcgc
    47461 aagactttgc tctcatttcg cacatcgcga cgctgaaaag ccgcgtgcca tttattcatt
    47521 tctttgatgg tttccgcacg tcccacgaaa tcaacaaaat tgtcccgctg gccgatgaca
    47581 cgattcttga tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg gcactcaacc
    47641 cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga cacttatttc cagtctcgcg
    47701 aagccaccaa cccatggtac gacgcggtct atgaccatgt tgaacaggcg atgaatgatt
    47761 tctctgccgc gacaggtcgt cagtatcagc cgtttgaata ttacgggcat ccgcaagcgg
    47821 aacgggtgat tatcctgatg ggctctgcca ttggcacctg tgaagaagtg gttgatgaat
    47881 tgctaacccg tggcgaaaaa gtcggcgtgc tgaaagttcg cctgtaccgc cccttctcag
    47941 ccaaacactt gctacaaact ctaccgggat ccgtacgtag cgtggcggta ctggacagaa
    48001 ccaaagaacc cggtgcccag gcagaaccgc tctatctgga tgtgatgacc gcactggcag
    48061 aagcctttaa taatggcaag cgcgaaactc tcccccgtgt cattggtggg cgctatggtc
    48121 tttcatccaa agaatttggc ccggactgtg tactggcggt atttgccgag ctcaacgcgg
    48181 ctaaaccgaa agcgcgcttt acggttggta tttacgatga tgtaaccaat ctgtccctgc
    48241 cgttgccgga aaacacccag ccaaactcgg cgaaacttga agccttgttt tatggccttg
    48301 gtagtgatgg cagcgtttcc gcgaccaaaa acaatatcaa gattatcggt aattccacgc
    48361 cgtggtacgc acagggctat tttgtttacg actccaaaaa ggcgggcggc ctgacggttt
    48421 ctcaccttcg agtgagcgaa cagccgattc gttccgctta tctcatttcc caggctgatt
    48481 ttgttggctg ccaccagttg cagtttatcg ataaatatca gatggctgag cgtttaaaac
    48541 ctggcggcat tttcctgctc aacacaccgt acagcgcaga tgaagtgtgg tcgcgcttgc
    48601 cgcaagaagt tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg attaacgcgg
    48661 cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg cagatggctt
    48721 ttttccatct gacgcaaatt ctgcctggcg atagcgccct cgcagaattg cagggtgcga
    48781 ttgccaaaag ttacagtagc aaaggccagg atctggtgga acgcaattgg caggctctgg
    48841 cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg cacagcgcca
    48901 atcgaccgcc agtggtttcc gatgccgctc ctgatttcgt gaaaaccgta accgctgcga
    48961 tgctcgccgg gcttggtgac gccctccccg tttcggcgct gccgccagac ggctcctggc
    49021 cgatgggcac tacgcgctgg gaaaaacgta atattgccga agagatcccc acctggaaag
    49081 aggaactctg tacccaatgt aaccactgcg ttgccgcttg cccacactca gctattcgcg
    49141 caaaagtggt gccgcctgaa gcgatggaaa acgcccctgc cagcctgcat tcgctggatg
    49201 tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg gaagattgca
    49261 ccggttgtaa cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat ccagagatta
    49321 aagccatcaa tatgatgtct cgcctggaac atgtcgaaga agagaaaatc aattacgatt
    49381 tcttcctcaa cctgccagaa atcgaccgta gcaaactgga acgtattgat attcgtacat
    49441 cgcagctgat tacaccgctg tttgaatatt caggtgcttg ctccggttgt ggcgagacgc
    49501 cgtatattaa attgctgact cagctctatg gcgaccggat gttgatcgct aacgccaccg
    49561 gctgttcttc aatttatggc ggtaacctgc cctctacacc gtataccacc gatgccaacg
    49621 gtcgtgggcc ggcatgggcg aactctctat ttgaagataa tgccgaattt ggccttggtt
    49681 tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat caatttgccg
    49741 ataaaatccc ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca gaggttcgtc
    49801 gagaacaggt cgcagcttta cgccagcaac tcaacgatgt tgccgaagca catgaactgc
    49861 tgcgtgacgc agatgcactg gtggaaaaat caatctggct gattggtggc gacggctggg
    49921 cttacgatat cggctttggc ggtctggatc atgtattgag tttgacggaa aacgtcaaca
    49981 ttctggtgct ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg aaagcgacac
    50041 cgctgggtgc agtaactaag ttcggcgagc acggcaaacg taaagcgcgt aaagatcttg
    50101 gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca gatttctctc ggcgcgcagc
    50161 tgaaccagac ggtgaaagcg attcaggaag cggaagcgta tccggggcca tcgctgatca
    50221 ttgcttatag cccgtgtgaa gagcatggtt acgatctggc actcagccat gaccagatgc
    50281 gccaactcac agctaccggc ttctggccgc tatatcgctt cgatcctcgt cgtgccgatg
    50341 aaggcaaact gccgctggcg ctggattcac gcccgccgtc agaagcactg gaagaaacgt
    50401 tacttcacga gcaacgtttc cgtcgtctga attcgcagca gccagaagtg gcagaacagt
    50461 tatggaaaga tgctgcagct gatttgcaaa aacgctatga cttcctggca caaatggccg
    50521 gaaaagcgga aaaaagcaac accgattaaa tgctctggat aaggattatc caattctaaa
    50581 aaaaaagccc ggacgactgt tcgggcttgt ctttttatat gttggaaaat cagtggcaat
    50641 gcaatggccc aacagaaaat aaaatctgtg acaataaagg catataaccc gcgcagaata
    50701 acgtatagaa aatt
//
LOCUS       DAZGJW010000017        48831 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZGJW010000017 DAZGJW010000000
VERSION     DAZGJW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.017"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(135..1337)
                     /gene="ubiI"
                     /locus_tag="ACHKIK_001043"
                     /gene_synonym="visC"
     CDS             complement(135..1337)
                     /gene="ubiI"
                     /locus_tag="ACHKIK_001043"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO9082259.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(1360..2538)
                     /gene="ubiH"
                     /locus_tag="ACHKIK_001044"
                     /gene_synonym="visB"
     CDS             complement(1360..2538)
                     /gene="ubiH"
                     /locus_tag="ACHKIK_001044"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO9082260.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(2535..3860)
                     /gene="pepP"
                     /locus_tag="ACHKIK_001045"
     CDS             complement(2535..3860)
                     /gene="pepP"
                     /locus_tag="ACHKIK_001045"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO9082261.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMHDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(3886..4464)
                     /gene="ygfB"
                     /locus_tag="ACHKIK_001046"
     CDS             complement(3886..4464)
                     /gene="ygfB"
                     /locus_tag="ACHKIK_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO9082262.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            4632..4961
                     /gene="zapA"
                     /locus_tag="ACHKIK_001047"
     CDS             4632..4961
                     /gene="zapA"
                     /locus_tag="ACHKIK_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO9082263.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            5003..5186
                     /gene="ssrS"
                     /locus_tag="ACHKIK_001048"
     ncRNA           5003..5186
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKIK_001048"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5207..5809
                     /gene="fau"
                     /locus_tag="ACHKIK_001049"
     CDS             5207..5809
                     /gene="fau"
                     /locus_tag="ACHKIK_001049"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO9082264.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(5910..5969)
                     /locus_tag="ACHKIK_001050"
     CDS             complement(5910..5969)
                     /locus_tag="ACHKIK_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO9082265.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(6302..6361)
                     /gene="ibsC"
                     /locus_tag="ACHKIK_001051"
     CDS             complement(6302..6361)
                     /gene="ibsC"
                     /locus_tag="ACHKIK_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO9082266.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(6590..7822)
                     /gene="serA"
                     /locus_tag="ACHKIK_001052"
     CDS             complement(6590..7822)
                     /gene="serA"
                     /locus_tag="ACHKIK_001052"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO9082267.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(8078..8737)
                     /gene="rpiA"
                     /locus_tag="ACHKIK_001053"
     CDS             complement(8078..8737)
                     /gene="rpiA"
                     /locus_tag="ACHKIK_001053"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO9082268.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(8793..8978)
                     /locus_tag="ACHKIK_001054"
     CDS             complement(8793..8978)
                     /locus_tag="ACHKIK_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9082269.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            9120..10013
                     /gene="argP"
                     /locus_tag="ACHKIK_001055"
     CDS             9120..10013
                     /gene="argP"
                     /locus_tag="ACHKIK_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO9082270.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            10217..12361
                     /gene="scpA"
                     /locus_tag="ACHKIK_001056"
     CDS             10217..12361
                     /gene="scpA"
                     /locus_tag="ACHKIK_001056"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO9082271.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            12354..13349
                     /gene="meaB"
                     /locus_tag="ACHKIK_001057"
     CDS             12354..13349
                     /gene="meaB"
                     /locus_tag="ACHKIK_001057"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO9082272.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            13360..14145
                     /gene="scpB"
                     /locus_tag="ACHKIK_001058"
     CDS             13360..14145
                     /gene="scpB"
                     /locus_tag="ACHKIK_001058"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO9082273.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAQLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            14169..15647
                     /gene="scpC"
                     /locus_tag="ACHKIK_001059"
     CDS             14169..15647
                     /gene="scpC"
                     /locus_tag="ACHKIK_001059"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO9082274.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15644..16540)
                     /gene="ygfI"
                     /locus_tag="ACHKIK_001060"
     CDS             complement(15644..16540)
                     /gene="ygfI"
                     /locus_tag="ACHKIK_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9082275.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(16707..17447)
                     /gene="yggE"
                     /locus_tag="ACHKIK_001061"
     CDS             complement(16707..17447)
                     /gene="yggE"
                     /locus_tag="ACHKIK_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO9082276.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(17540..18175)
                     /gene="argO"
                     /locus_tag="ACHKIK_001062"
     CDS             complement(17540..18175)
                     /gene="argO"
                     /locus_tag="ACHKIK_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO9082277.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(18314..19174)
                     /gene="mscS"
                     /locus_tag="ACHKIK_001063"
     CDS             complement(18314..19174)
                     /gene="mscS"
                     /locus_tag="ACHKIK_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO9082278.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            19370..19468
                     /locus_tag="ACHKIK_001064"
                     /pseudo
     CDS             19370..19468
                     /locus_tag="ACHKIK_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19532..20611)
                     /gene="fbaA"
                     /locus_tag="ACHKIK_001065"
     CDS             complement(19532..20611)
                     /gene="fbaA"
                     /locus_tag="ACHKIK_001065"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO9082279.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(20826..21989)
                     /gene="pgk"
                     /locus_tag="ACHKIK_001066"
     CDS             complement(20826..21989)
                     /gene="pgk"
                     /locus_tag="ACHKIK_001066"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO9082280.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(22039..23058)
                     /gene="epd"
                     /locus_tag="ACHKIK_001067"
     CDS             complement(22039..23058)
                     /gene="epd"
                     /locus_tag="ACHKIK_001067"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO9082281.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            23430..23861
                     /locus_tag="ACHKIK_001068"
     CDS             23430..23861
                     /locus_tag="ACHKIK_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO9082282.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            23884..24462
                     /locus_tag="ACHKIK_001069"
     CDS             23884..24462
                     /locus_tag="ACHKIK_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO9082283.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTNPIAAAFAPVAMVIGLVAGCLAREGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            24463..25170
                     /locus_tag="ACHKIK_001070"
     CDS             24463..25170
                     /locus_tag="ACHKIK_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO9082284.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATQ
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            25158..25835
                     /locus_tag="ACHKIK_001071"
     CDS             25158..25835
                     /locus_tag="ACHKIK_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9082285.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            25829..26506
                     /locus_tag="ACHKIK_001072"
     CDS             25829..26506
                     /locus_tag="ACHKIK_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9082286.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(26478..27191)
                     /locus_tag="ACHKIK_001073"
     CDS             complement(26478..27191)
                     /locus_tag="ACHKIK_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO9082287.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(27188..27697)
                     /gene="fumE"
                     /locus_tag="ACHKIK_001074"
     CDS             complement(27188..27697)
                     /gene="fumE"
                     /locus_tag="ACHKIK_001074"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO9082288.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(27719..28684)
                     /gene="glpX"
                     /locus_tag="ACHKIK_001075"
     CDS             complement(27719..28684)
                     /gene="glpX"
                     /locus_tag="ACHKIK_001075"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO9082289.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(28681..29958)
                     /gene="yggP"
                     /locus_tag="ACHKIK_001076"
     CDS             complement(28681..29958)
                     /gene="yggP"
                     /locus_tag="ACHKIK_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO9082290.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(29973..31361)
                     /gene="cmtA"
                     /locus_tag="ACHKIK_001077"
     CDS             complement(29973..31361)
                     /gene="cmtA"
                     /locus_tag="ACHKIK_001077"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO9082291.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(31389..31832)
                     /gene="cmtB"
                     /locus_tag="ACHKIK_001078"
     CDS             complement(31389..31832)
                     /gene="cmtB"
                     /locus_tag="ACHKIK_001078"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO9082292.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            complement(32146..34137)
                     /gene="tkt"
                     /locus_tag="ACHKIK_001079"
     CDS             complement(32146..34137)
                     /gene="tkt"
                     /locus_tag="ACHKIK_001079"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO9082293.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            34415..35173
                     /gene="loiP"
                     /locus_tag="ACHKIK_001080"
                     /gene_synonym="yggG"
     CDS             34415..35173
                     /gene="loiP"
                     /locus_tag="ACHKIK_001080"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO9082294.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(35229..35972)
                     /locus_tag="ACHKIK_001081"
     CDS             complement(35229..35972)
                     /locus_tag="ACHKIK_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO9082295.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(35959..37068)
                     /gene="dgaE"
                     /locus_tag="ACHKIK_001082"
     CDS             complement(35959..37068)
                     /gene="dgaE"
                     /locus_tag="ACHKIK_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HFO9082296.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGFITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGDIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(37072..37932)
                     /locus_tag="ACHKIK_001083"
     CDS             complement(37072..37932)
                     /locus_tag="ACHKIK_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO9082297.1"
                     /translation="MMSDDVMQHELVERARESGALTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEAINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(37929..38678)
                     /locus_tag="ACHKIK_001084"
     CDS             complement(37929..38678)
                     /locus_tag="ACHKIK_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFO9082298.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(38704..39189)
                     /locus_tag="ACHKIK_001085"
     CDS             complement(38704..39189)
                     /locus_tag="ACHKIK_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO9082299.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(39200..39628)
                     /locus_tag="ACHKIK_001086"
     CDS             complement(39200..39628)
                     /locus_tag="ACHKIK_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO9082300.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(39747..42545)
                     /gene="dagR"
                     /locus_tag="ACHKIK_001087"
     CDS             complement(39747..42545)
                     /gene="dagR"
                     /locus_tag="ACHKIK_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HFO9082301.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(42804..43724)
                     /gene="speB"
                     /locus_tag="ACHKIK_001088"
     CDS             complement(42804..43724)
                     /gene="speB"
                     /locus_tag="ACHKIK_001088"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO9082302.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(43860..44591)
                     /locus_tag="ACHKIK_001089"
     CDS             complement(43860..44591)
                     /locus_tag="ACHKIK_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9082303.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(44737..46713)
                     /gene="speA"
                     /locus_tag="ACHKIK_001090"
     CDS             complement(44737..46713)
                     /gene="speA"
                     /locus_tag="ACHKIK_001090"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO9082304.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(46722..46853)
                     /gene="yqgB"
                     /locus_tag="ACHKIK_001091"
     CDS             complement(46722..46853)
                     /gene="yqgB"
                     /locus_tag="ACHKIK_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO9082305.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(46947..47090)
                     /gene="yqgG"
                     /locus_tag="ACHKIK_001092"
     CDS             complement(46947..47090)
                     /gene="yqgG"
                     /locus_tag="ACHKIK_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO9082306.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(47201..47452)
                     /gene="yqgD"
                     /locus_tag="ACHKIK_001093"
     CDS             complement(47201..47452)
                     /gene="yqgD"
                     /locus_tag="ACHKIK_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO9082307.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            47508..48662
                     /gene="metK"
                     /locus_tag="ACHKIK_001094"
     CDS             47508..48662
                     /gene="metK"
                     /locus_tag="ACHKIK_001094"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO9082308.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT        11914 a        13263 c        12141 g        11513 t
ORIGIN      
        1 aattcgtgac attagaaaat ataatgcgaa aacggaggtg aaattagttt atttcaaatg
       61 aggaaaatct cccggcgaaa aaaccgggag atgaaagtgt gatgggtatc aaataaacag
      121 cagaagagaa atttttaacg cagccattca ggcaaatcgt ttaatcccat tgcctggcgg
      181 ataagttgtg gtttaacgcc aggaagcgtg tcggccagtt tcaaaccaat atcacgcagc
      241 agttttttcg ccggattggt accggaaaac agatcgcgga atccctgcat accagccagc
      301 atcagcgccg cactgtgctt gcggctacgc tcataccggc gcaggtaaat gtactgcccg
      361 atgtctttcc cctgacgatg caaccgtttc agttcagcaa tcagctctgc agcatccata
      421 aagccgagat ttactccctg ccccgccagc gggtgaatgg tatgcgcggc gtcgcccacc
      481 agcaccagac ggtgcgcggc aaactggcgc gcataacgcc ccgtcagtgg gaacacctga
      541 cgcgcgctct caaccttgca taagcccagg cgattatcaa aagcgatatt taacgcgcga
      601 ttaaattcat cttcacttgc ctgctgcatc cgctgcgctt cctctggcga cagtgaccag
      661 acaattgagc aaagatgcgg atcgctaagc ggtaaaaagg ccagaatgcc ttcgccatgg
      721 aaaacctgcc gcgccaccgc atcatgcggt tcttccgtgc gaatggtcgc taccagcgcg
      781 tgatgctgat aatcccagaa agtcagcgga atatctgctt tgttgcgcaa ccaggaatta
      841 gcaccgtccg cgccaatcac cagacgcgcc gttaacatgc tgccatcttt cagcgtcagg
      901 aaagtttcat tttctcccca ggcgacctgc tgtaattctg cgggggccaa cagagtgata
      961 tctgacgact gctgcgcttt gttccacagc gcgtagtgaa tcactgagtt ttcaacgata
     1021 tgcccaagat ggctatagcc catgctttga tcgtcaaacg aaatgtgacc aaaactgtct
     1081 ttgtcccaca cttccatacc gtgataacaa ctggccctac gagagagaat gtcctgccag
     1141 acgccaagac gggtgagtaa tttttcgctg gcggcattga tagccgaaac gcgcagttgt
     1201 ggtggtgcat ccgccgccag aggttccggt acgcgctgct ccagtacggc aacgcgtaag
     1261 ccgctcccct gtaagccgca ggcaaccgcc agccccacca tgccgccacc aacaatggct
     1321 acatcaacac tttgcattgt ttattcctta aaaccgcctt caacgcgcca cccaaccgag
     1381 ggtgcgctgc gccagcacat cgcgtgccgg ggtgaataat tccatcgtca tcagtccgat
     1441 gttgcgcccg acaaccagcg gtgcccaacg gttggcaaaa agatgtacca ggctgtccgt
     1501 gacgccgatg gttgcttcgc gatcactctg tcgacgctgc tgatagcggc acaatatgcc
     1561 gtaatcaccg atgtcttctc cgcgctcatg cgcctgagtc agggtttccg caaggctcat
     1621 gacatctcgc ataccgaggt taaacccttg cccggcaatc gggtgcagag tttgcgccgc
     1681 attgcccacc agcacggtac gatgggtaat agctctggcg gcgcgggtta acgccagcgg
     1741 ataagcactg cgtttaccgg cgtgggtaat ttttccaagt cgccagccaa aggccgactg
     1801 gagttcacgg caaaacttct cgtcgctcca cgacagcacc tcttcgcgcc gttccagtgg
     1861 atgacaccag accagcgaac agcgtccgtc agacatcggc aacatcgcca gcgggccatg
     1921 ttgcgtgaag cgttcaaaag cgcggccttc gtgcgcaacg gaagtcgcaa cgttggcaat
     1981 cacggccagt tgttcgtaag gctcctgctg ccagtcaacg ccgcacgcgg tggctaacgc
     2041 tgaatgggtg ccatcagctg ctaccagcac gcggcccgtc agcgtctcgc cactctccag
     2101 cgtcacttca acgtgactct gagtacgggc aacgttagcc acgcgatcag ggcaatgcag
     2161 cgttacgcca ggtgctttac gcagcaatgc aaacagccgt tgcccgacat tgtgcaattc
     2221 gacaacctgt cccagcgccg ccagttggta atcttctgcg gcgagggtga caaatccagc
     2281 gtgaccacga tcgctgacat gcacggtagt gatggcggtt gcgtaatccg ccagagattg
     2341 ccagacgccg atgcgcgcca gttgctgaca ggtccccgca gccagcgcaa tagctcgtcc
     2401 atcaaagccc ggatgagcat gtgactctgg cgcggtcgct tcaatcaaat gtaccggcag
     2461 cgccccgtga cttaaccggg aaatagccag cgccagcgtc gcgcccgcca tgccgccacc
     2521 gacgatgatt acgctcattg ctttctcgca gctgccatca acgcttcgat ttcttccggc
     2581 tttttcacca cgctggcggt gaggttttcg ttgccggttt cggtaatcac aatgtcgtct
     2641 tcaatacgaa tgccgatacc gcgatattgt tctggcacat ccgcatccgg cgcgatatac
     2701 agaccaggct ctacggtcag taccatcccc ggttccagaa tgcgcgagcg atcctgacca
     2761 taaacgccaa cgtcatggac atctagccct aaccagtggc taaggccatg cataaagaaa
     2821 ggacgatggg cgttctgagc gatcagttca tcaacatcac ctttcagaat gccgagtttt
     2881 accaggccgc taaccatgat gcgcaccact tcaccagtga cttccagaat ggaagttccc
     2941 ggacgataca ggcgcaagct ggtttcgaga gactccagca caatgtcgta gatttcacgc
     3001 tgggcctgag tgaatttgcc gttgaccggg aaggtgcggg taatatcgcc agcgtaacct
     3061 ttgtattcgc aacccgcgtc aatcaacacc aggtcgccgt cgtgcatttc acactcgttt
     3121 tcggtgtagt gcagaatgca gccgttttca ccgctgccga caatggtgtt ataggacgga
     3181 tagcgcgcac cgtggcggtt aaattcgtgg tgaatttcgc cttccagatg gtactcgaac
     3241 attcccggac ggcatttttc catcgcccgg gtatgtgcca tggcggtgat ttctcccgcg
     3301 cggcggagta cggcaatctc ttctggcgat ttgaacaggc gcatttcatg aacaacagga
     3361 cgccagtcga tcatcgttgc cggtgcggtg agattttgcc gcgaaccttt acgcagtttt
     3421 tccagcgcac tgttcacgat ttcatcagca tatgcatatt cgccctgggc atggtaaacc
     3481 acatccaggc cgttaagtag ttgataaagt tgctgattga tttcgctgaa tgccagtgcg
     3541 cggtcaacgc ccagtttctc tggcgcggca tcctggccta agcgacggcc aaaccagatc
     3601 tccgccgtca ggtcgcgaac gcggttaaac agaacgctgt ggttatgagt gtcatcgctt
     3661 ttaatcagca ccagcaccgc ttccggttcg ttaaagccgg tgaagtacca gaagtcactg
     3721 ttctgacgat aggggtattc gctgtcggcg ctacgtgtta cttctggtgc agcaaaaatc
     3781 agcgcggcgc tgccgggttg catttgctcc accagggcct gacggcgacg ctgaaactct
     3841 tgccgggata tctcactcat aacactctcc ttacgttttt tgtttttagt gtagagtcgg
     3901 tttttgtact tctggcgcgg tcggttgcgg atgagtaaag gtgtcgtggc ataacagcgc
     3961 ggcaacacga acgtattcga tgatctcttc aagcgacatt tcaagctctt cctgatcttc
     4021 gtcttcgtcg taacccagtt gcgcaatgtt acgcagatcg tcgatagctt caccggtttc
     4081 gccggtcact ttatccagct tcggttgcgt aacgccaaga ccaagcagga agtgattgac
     4141 ccaacctgcc aatgcatcag cccgatcgaa aacgctgaca tcatcgccat caggcagata
     4201 aagctgaaaa aggaagccgt catcctgcag ggcatcgctg gtggcagagt gcattttacg
     4261 cagtgcctgt gccagctcat gaccgaaagc catgccttcg ttcgtcaggt cgtgaagtag
     4321 cggtagccat gagctgtcat cgttaccgcc acatatcatc ccactgatta aaccatgcat
     4381 ctcagcaggg gtcagacccg tcccttgttg gttcagatac tggttcattt cgttgtaacc
     4441 aggcatttcg ttctgtatag acataagcat tcgtcgtcaa agggaggaat attcatgata
     4501 tgctaccact ttgggccctg gtggaccaga aaagggcttg tctcttctca tcagggtagc
     4561 tatagtgtcg ccccttcgca gaccatgggt ctaaagacga aggcagcgca gtcaatcagc
     4621 aggaaggtgg catgtctgca caacccgtcg atatccaaat ttttggccgt tcactgcgtg
     4681 tgaactgccc gcctgaccaa agggatgcgt tgaatcaggc agcggacgat ctgaaccaac
     4741 ggttgcaaga tctgaaagaa cgcactagag tcacaaatac tgaacagttg gtcttcattg
     4801 ccgcattgaa tatcagctat gagttagcgc aagaaaaagc gaagactcgt gactacgcgg
     4861 caagtatgga acagcgtatt cggatgctgc agcagaccat agaacaagcg ttacttgaac
     4921 aaggtcgcat caccgaaaaa actaaccaaa actttgaatg acacttttcg gtttactgtg
     4981 gtagagtaac cgtgaagaca aaatttctct gagatgttcg caagcgggcc agtcccctga
     5041 gccgatattt cataccacaa gaatgtggcg ctccgcggtt ggtgagcatg ctcggtccgt
     5101 ccgagaagcc ttaaaactgc gacgacacat tcaccttgaa ccaagggttc aagggttaca
     5161 gcctgcggcg gcatctcgga gattcccttc ttatctggca ccagccatga cgcaactacc
     5221 agaactccca ctgacattat cccgacaaga aatccgcaaa atgattcggc aacgtcgtcg
     5281 tgcgttaacg ccggaacaac agcaggaaat gggtcaacaa gccgctaccc ggatgatgac
     5341 ttatcccccg gtggtgatgg cgcatacggt cgctgtattc ctctcttttg atggtgaact
     5401 cgacacccag ccgctaatag aacaactctg gcgcgccggt aagcgcgtat atcttccagt
     5461 tttgcatccc tttagtgccg gtaatttgct gttcctgaat taccatccgc aaagcgaact
     5521 ggtgatgaac aggttgaaga tccatgagcc aaaactggat gtgcgtgacg tgctacccct
     5581 ttcccgatta gacgtgctga tcacaccgct ggtcgccttt gatgagtacg gtcagcgcct
     5641 gggaatgggc ggtggttttt atgatcggac cttacaaaac tggcagcact ataaaacgca
     5701 accggtgggt tatgcgcatg attgtcagtt ggtggaaaaa ctccccgttg aagagtggga
     5761 tatccctctt cctgcggtgg ttacaccgtc gaaagtctgg gagtggtaag ggcgatacac
     5821 ctgcatcgcc ctgattgaca tcgttgattc tttaacctaa tttagtgagt aagggtaagg
     5881 gaggattgct cctcccctgt gactgactgt taataagctg ggaaacttat gagtaacagt
     5941 acaatcagta tgatggcaag tcgcatcata acccttctcc ttcaagccct cgcttcggtg
     6001 agggctttac cgttacagcc ccatgctgcc cagctatcgt aagctctctt taaataacca
     6061 caacgcattg atctaccttt aaatcttttc ttcgagcgtg ctcgaaaaac cccatgcaaa
     6121 acagagataa tttttgggaa aaaggatcta ttacggcagt aaacgtgggc gatgaagtgg
     6181 ctatcaaaaa gttcggttca gggcgatgca ccccgcatcg ccctgattga catcgttgat
     6241 tctttgacct aatttagtga gtaagggtaa gggaggatga ctcctcccct gagactgact
     6301 gttaataagc tccgaaactt atcagtaaca gtacaatcag tatgatgaca agtcgcatca
     6361 taacccttct cctttaagcc ctcgcttcgg tgagggcttt accgttacag ccccatgctg
     6421 tcccgccatc gtaaatcccc attaaataaa cacaacgcat tgatctgact ttgatttatt
     6481 ttctggagca gactcgcaaa gtagaatgcg caacgcggca acggtgtgga gaagggataa
     6541 aaaaacgggc aagtcagtga cctgcccgtt aattttcaga gaaggggaat tagtacagca
     6601 gacgggcgcg aatggtaccc ggaatagctt tcattgcctg cagcgctttt tcggcaacgt
     6661 cttcgtcagc ttcaatatca ataactacat aacccatctg ggcggaagtt tgtagatatt
     6721 gcgcggcgat gttgacgccc tgctcggcga agattttgtt cagcgcagtt agcacgcccg
     6781 gacggttttc gtggatgtgc atcagacgac gaccaccatg cagtggcagc gagacttccg
     6841 ggaagttcac cgcagagagc gttgagccat tgtcagaata cttgatcaat ttacccgcaa
     6901 cttccaggcc gatattctcc tgcgcttcct gagtcgaacc gccaatgtgt ggcgtcagaa
     6961 ggacgttgtc gaattcacac agtggagagg taaatggatc gctattggtc gccggttccg
     7021 tcgggaatac gtcgattgcc gcccccgcca gatgtttgct cgccagcgca tcacacagcg
     7081 ccggaatatc caccacagta ccgcgcgagg cattaatcag cagcgagccg ggcttcatta
     7141 gcgaaatctc tttcgcgccc atcatatttt tggtggacgg attctctggt acatgcagac
     7201 tcaccacatc gctcatattc agcaggtcag aaagatgctg tacctgagtg gcgttgccca
     7261 gcggcagttt gttttcaata tcataaaagt aaacatacat tcccagcgat tcagccagaa
     7321 tgcccaattg cgtaccaata tgaccgtagc cgatgatacc cagctttttg ccgcgcgctt
     7381 caaaagaacc cgccgccagt ttgttccaca cgccacggtg cgctttagca ttggcttccg
     7441 gcacgccgcg caatagcagc agcagttcgc caatcaccag ctccgcaaca gagcgcgtat
     7501 ttgagaacgg tgcgttaaat accgggatcc cgcgctttgc cgccgcatcc agatcaacct
     7561 ggtttgttcc gatacagaaa cagccaatag cgaccagttt ttctgcggcg ttgatcacgt
     7621 cttcagtcag atgggtacgg gatcgcaggc cgatgaagtg ggcatcgcgg atggattctt
     7681 ttaattgttc atcatccagc gcgcctttgt gaaattcgat gttggtgtaa ccagctgcac
     7741 gaaggctttc cagcgccttt tggtgcacgc cttctaccag cagaaactta atcttgtctt
     7801 tctccagcga tacctttgcc atttacccaa tcctgtcttt tgaaatgttg tgtgcggatt
     7861 tgcatccgcc tttcaacata tcaaaaaata atattgcggc aatatgaacg tttgcgtcgc
     7921 gatgttgagg aaatatcacg caaaggtaat tcttggatga atatgctggt ttagaggatt
     7981 tatttagagc aatcgacaat tgcctggtaa aagcgtgaca catgtcacca aatttaatga
     8041 agagaatttt tttaacgggg gaggttcccc cgtcagatca tttcacaatg gttttgacac
     8101 cgtcaggtgt gccaatcagc gcaacgtccg cgccacggtt agcaaacaag ccaacagtca
     8161 ccacgccagg aatcgcattt atggcgtttt ccatcgctat cgggtcaagg atttccatgc
     8221 cgtggacgtc gaggatcacg ttgccattat cggtcaccac gccctgacgg tattccggac
     8281 gaccgcccag tttcaccagc tgacgcgcca ctgcactacg tgccatcggg ataacttcta
     8341 ctggcagcgg gaatttaccc agaatatcaa cctgcttgga agcgtctgca atacagataa
     8401 atttttctgc aaccgaagca atgatttttt cacgggtcag cgccgcgccg ccgcctttga
     8461 tcatttgcat gtggccgttg atttcatctg cgccatcaac gtagatgcca aggctgtcga
     8521 cttcgttgag atcaaaaacg tgaatgccga ggcttttcag tttttcagtg gaagcatctg
     8581 aactggaaac ggccccttca atctggcctt tcattgtacc gagcgcgtca ataaagtgtg
     8641 cggcggtgga acctgtacct acaccaacaa tggtgccggg ctgaacatac tgaagtgccg
     8701 cccatcctac tgcttttttc aattcatcct gcgtcatgat cgtttcgcct gtggtatgaa
     8761 atttcacacg cattatatac aaaaaaagcg attcagaccc cgttggcaag ccgcgtggtt
     8821 aactcatcca taaaatatcg cgcaatggcg ggcatcccct ttcgccccgc aaataaagca
     8881 tacaacggtc tgggtatgcc gctccacggt gcgaacagag gcaataacgc cacgccattc
     8941 aacacaacca gggcgcgaac tcgctgagcg aaatgcatta gcgcaaatcc ctgatctttt
     9001 aagctgcgcg tttaaccacg aatacaaatg ttacccgccg acggcttcgg tatatgcaac
     9061 ctgacacaaa attgtgtcat agtgcaggaa aaagcattta ccaggagcag acaacagcaa
     9121 tgaaacgccc ggactacaga acattacagg cactggatgc ggtgatacgt gaacgaggat
     9181 ttgagcgcgc ggcacaaaag ctgtgcatta cacaatcagc cgtctcacag cgcattaagc
     9241 aactggaaaa tatgttcggg cagccgctgt tggtgcgtac cgtaccgccg cgcccgacgg
     9301 aacaagggca aaaactgctg gcactgctgc gccaggtgga gttgctggaa gaagagtggc
     9361 tgggcgatga acaaaccggt tcgactccgc tgctgctttc actggcggtc aacgccgaca
     9421 gtctggcgac gtggttgctt cctgcactgg ctcctgtgtt ggctgattcg cctatccgcc
     9481 tcaacttgca ggtagaagat gaaacccgca ctcaggaacg tctgcgccgc ggcgaagtgg
     9541 tcggcgcggt gagtattcaa catcaggcgc tgccgagttg tcttgtcgat aaacttggtg
     9601 cgctcgacta tctgttcgtc agctcaaaac cctttgccga aaaatatttc cctaacggcg
     9661 taacgcgttc ggcattactg aaagcgccag tggtcgcgtt tgaccatctt gacgatatgc
     9721 accaggcctt tttgcagcaa aacttcgatc tgcctccagg cagcgtgccc tgccatatcg
     9781 ttaattcttc agaagcgttc gtacaacttg ctcgccaggg caccacctgc tgtatgatcc
     9841 cgcacctgca aatcgagaaa gagctggcca gcggtgaact gattgactta acgcctgggc
     9901 tatttcaacg acggatgctc tactggcacc gctttgctcc tgaaagccgc atgatgcgta
     9961 aagtcactga tgcgttactc gattatggtc acaaagtcct tcgtcaggat taatccatca
    10021 aataatgcct gatagcacat atcaggcgtt gtcctcactt ctttttgtat tccttgaatc
    10081 acatcacaaa atagacaaat ctcaggcggc aaaaaacgac gtctgaatgc attttttttg
    10141 ctggcgacaa acccacgtaa aaagctcacc gtaggcgcaa ataccctcat tttgattgcg
    10201 ttttacggag caaataatgt ctaacgtgca ggagtggcaa cagcttgcca acaaggaatt
    10261 gagccgtcgg gagaaaactg tcgactcgct ggttcagcag accgcggaag ggatcgccat
    10321 caagccgctg tataccgaag ccgatctcga taatctggag gtgacaggta cccttcctgg
    10381 tttgccgccc tacgttcgtg gcccgcgtgc cactatgtat accgcccaac cgtggaccat
    10441 ccgtcagtat gctggttttt caacagcaaa agagtccaac gctttttatc gccgtaacct
    10501 ggccgccggg caaaaaggtc tttccgttgc gtttgacctt gccacccacc gtggctacga
    10561 ctccgataac ccgcgcgtgg cgggcgacgt cggcaaagcg ggcgtcgcta tcgacaccgt
    10621 ggaagatatg aaagtcctgt tcgaccagat cccgctggat aaaatgtcgg tttcgatgac
    10681 catgaatggc gcagtgctac cagtactggc gttttatatc gtcgccgcag aagagcaagg
    10741 tgttacacct gataaactga ccggcaccat tcagaacgat atcctcaaag agtacctctg
    10801 ccgcaacacc tatatttacc caccaaaacc gtcaatgcgc attatcgccg acatcatcgc
    10861 ctggtgttcc ggcaacatgc cgcgatttaa taccatcagt atcagcggtt accacatggg
    10921 ggaagcgggt gccaactgcg tgcagcaggt agcatttacg ctcgctgatg ggattgagta
    10981 catcaaagca gcaatctctg ccggactgaa aattgatgac ttcgctcctc gcctgtcgtt
    11041 cttcttcggt atcggcatgg atctgtttat gaacgtcgcc atgttgcgtg cggcacgtta
    11101 tttatggagc gaagcggtca gtggatttgg cgcacaggat ccgaaatcac tggcgctgcg
    11161 tacccactgc cagacctcag gctggagcct gactgaacag gatccgtata acaacgttat
    11221 ccgcaccacc attgaagcac tggctgcgac gctgggcggt actcagtcac tgcataccaa
    11281 cgcctttgac gaagcgcttg gtttgcctac cgatttctca gcacgcattg cccgcaacac
    11341 ccagatcatc atccaggaag aatcagaact ctgccgcacc gtcgatccac tggccggatc
    11401 ctattacatt gagtcgctga ccgatcaaat cgtcaaacaa gccagagcta ttatccaaca
    11461 gatcgacgaa gccggtggca tggcgaaagc gatcgaagca ggtctgccaa aacgaatgat
    11521 cgaagaggcc tcagcgcgcg aacagtcgct gatcgaccag ggcaagcgtg tcatcgttgg
    11581 tgtcaacaag tacaaactgg atcacgaaga cgaaaccgat gtacttgaga tcgacaacgt
    11641 gatggtgcgt aacgagcaaa ttgcttcgct ggaacgcatt cgcgccaccc gtgatgatgc
    11701 cgccgtaacc gccgcgttga acgccctgac tcacgccgca cagcataacg aaaacctgct
    11761 ggctgccgct gttaatgccg ctcgcgttcg cgccaccctg ggtgaaattt ccgatgcgct
    11821 ggaagtcgct ttcgaccgtt atctggtgcc aagccagtgt gttaccggcg tgattgcgca
    11881 aagctatcat cagtctgaga aatcggcctc cgagttcgat gccattgttg cgcaaacgga
    11941 gcagttcctt gccgacaatg gtcgtcgccc gcgcattctg atcgctaaga tgggccagga
    12001 tggacacgat cgcggcgcga aagtgatcgc cagcgcctat tccgatctcg gtttcgacgt
    12061 agatttaagc ccgatgttct ctacacctga agagatcgcc cgcctggccg tagaaaacga
    12121 cgttcacgta gtgggcgcat cctcactggc tgccggtcat aaaacgctga tcccggaact
    12181 ggtcgaagcg ctgaaaaaat ggggacgcga agatatctgc gtggtcgcgg gtggcgtcat
    12241 tccgccgcag gattacgcct tcctgcaaga gcgcggcgtg gcggcgattt atggtccagg
    12301 tacacctatg ctcgacagtg tgcgcgacgt actgaatctg ataagccagc atcatgatta
    12361 atgaagccac gctggcagaa agtattcgcc gcttacgtca gggtgagcgt gccacactcg
    12421 cccaggccat gacgctggtg gaaagccgtc acccgcgtca tcaggcacta agtacgcagc
    12481 tgcttgatgc cattatgccg tactgcggta acaccctgcg actgggcgtt accggcaccc
    12541 ccggcgcggg gaaaagtacc tttcttgagg cctttggcat gttgttgatt cgagagggat
    12601 taaaggtcgc ggttattgcg gtcgatccca gcagcccggt cactggcggt agcattctcg
    12661 gggataaaac ccgcatgaat gacctggcgc gtgccgaagc ggcgtttatt cgcccggtac
    12721 catcctccgg tcatctgggc ggtgccagtc agcgagcgcg ggaattaatg ctgttatgcg
    12781 aagcagcggg ttatgacgta gtgattgtcg aaacggttgg cgtcgggcag tcggaaacag
    12841 aagtcgcccg catggtggac tgttttatct cgttgcaaat tgccggtggc ggcgatgatc
    12901 tgcagggcat taaaaaaggg ctgatggaag tggctgatct gatcgttatc aacaaagacg
    12961 atggcgataa ccataccaat gtcgccattg cccggcatat gtacgagagt gccctgcata
    13021 ttctgcgacg taaatacgac gaatggcagc cacgggttct gacttgtagc gcactggaaa
    13081 aacgtggaat cgatgagatc tggcacgcca tcatcgactt caaaaccgcg ctaactgcca
    13141 gtggtcgttt acaacaagtg cggcaacaac aatcggtgga atggctgcgt aagcagaccg
    13201 aagaagaagt actgaatcac ctgttcgcga atgaagattt cgatcgctat taccgccaga
    13261 cgcttttagc ggtcaaaaac aatacgctct caccgcgcac cggcctgcgg cagctcagtg
    13321 aatttatcca gacgcaatat tttgattaaa ggaattttta tgtcttatca gtatgttaac
    13381 gttgtcacta tcaacaaagt ggcggtcatt gagtttaact atggccgcaa acttaatgcc
    13441 ttaagtaaag tctttattga tgatcttatg caggcattaa gcgatctcaa ccggccggaa
    13501 attcgctgta tcattttgcg cgcgccgagt ggatccaaag tcttctccgc aggccacgat
    13561 attcacgaac tgccgtctgg cggtcgcgat ccgctctcct atgatgatcc attgcgtcaa
    13621 atcacccgca tgatccaaaa attcccgaaa ccgatcattt cgatggtgga aggtagtgtt
    13681 tggggtggcg catttgaaat gatcatgagt tccgatctga ttatcgccgc cagtacctca
    13741 accttctcaa tgacgcctgt aaacctcggc gtcccgtata acctggtcgg cattcacaac
    13801 ctgacccgcg acgcgggctt ccacattgtc aaagaactga tttttaccgc ttcgccaatc
    13861 accgcccagc gcgcgttggc tgtcggcatc ctcaaccatg ttgtggaagt ggaagaactg
    13921 gaagatttca ccttacaaat ggcgcaccac atctctgaga aagcgcagtt agccattgct
    13981 gttatcaaag aagagctgcg tgtactgggc gaagcgcaca ccatgaactc cgatgaattt
    14041 gaacgtattc aggggatgcg acgcgcggtt tatgacagcg aagattacca ggaaggaatg
    14101 aacgctttcc tcgaaaaacg taaacctaat ttcgttggtc attaatccct gcgaacgaag
    14161 gagtaaaaat ggaaactcag tggacaagga tgaccgccga tgaagcggca gaaattatcc
    14221 agcataacga catggtggca tttagcggct ttaccccggc gggttcgccg aaagccctac
    14281 ccaccgcgat tgcccgcaga gctaacgaac agcatgaggc caaaaagccg tatcaaattc
    14341 gcctgctgac gggggcgtca atcagcgccg ccgctgacga tgtactttct gacgccgatg
    14401 ctgtttcctg gcgtgcgcca tatcaaacat cgtccggttt acgtaaaaag atcaatcagg
    14461 gcgcggtgag tttcgttgac ctgcatttga gcgaagtggc gcaaatggtc aattacggtt
    14521 tcttcggcga cattgatgtt gccgtcattg aagcatcggc actggcaccg gatggtcgag
    14581 tctggttaac cagcgggatc ggtaatgcgc cgacctggct gctgcgagcg aagaaagtga
    14641 tcattgaact taatcactat cacgatccgc gcgttgcaga actggcagat attgtgattc
    14701 ctggcgcgcc accgcggcgc aatagcgtgt cgatcttcca tgcaatggat cgcgtcggta
    14761 cccgctatgt gcaaatcgat ccgaaaaaga ttgtcgccgt cgtggaaacc aacttgcccg
    14821 acgccggtaa tatgctggat aagcaaaatc ccatgtgcca gcagattgcc gataacgtgg
    14881 tcacgttctt attgcaggaa atggcgcatg ggcgtattcc gccggaattt ctgccgctgc
    14941 aaagtggcgt gggcaatatc aataatgcgg taatggcgcg tctgggggaa aacccggaaa
    15001 ttcctccgtt catgatgtat tcggaagtgc tacaggaatc ggtggtgcat ttactggaaa
    15061 ccggcaaaat cagcggggcc agcgcctcca gcctgacaat ctcggccgat tccctgcgca
    15121 agatttacga caatatggat tactttgcca gccgcattgt gttgcgtccg caggagattt
    15181 ccaataaccc ggaaatcatc cgtcgtctgg gcgtcatcgc tctgaacgtc ggcctggagt
    15241 ttgatattta cgggcatgcc aactcaacgc acgtagccgg ggtcgatctg atgaacggca
    15301 tcggcggcag cggtgatttt gaacgcaacg cgtatctgtc gatctttatg gctccgtcga
    15361 ttgctaaaga aggcaagatc tcaaccgtcg tgccaatgtg cagccatgtt gatcacagcg
    15421 aacacagcgt caaagtgatc atcaccgaac aagggatcgc cgatctgcgc ggtctttccc
    15481 cgcttcaacg cgcccgcact atcattgata attgtgcaca tcctatgtat cgggattatc
    15541 tgcatcgcta tctggaaaat gcgcccggcg gacatattca ccacgatctt agccacgtct
    15601 tcgacttaca ccgtaattta attgcaaccg gctcgatgct gggttaatgg tttgtcccgt
    15661 cacgatcttc agcaatatgc tgaagcatcg tggcggtata acgatgattt ctgattgaat
    15721 aaagatattc ctgcatatac atcggcctgt gcggcgcatc aaaatatttc agcgttgccg
    15781 gattcaccag tcgccaggca aaatgcggga tcaccgtcag aaaatgtccg cgctccacgg
    15841 cgctgatttt ggccataaaa ctgtagggac gataaataat ggtgggattg atcccacaag
    15901 gtcgcatatt cgcatcaatc attgcttcga agttagcccg gttttgaaag cgcatttgta
    15961 gccagggcag ctcctgaagc agctcttgct cttgacgctc ttcataacgg cgagaaacga
    16021 gaaagcccaa tcgcagcggt ggtaattcac tgatagtcag gttttccagt tcctgaactc
    16081 gtgcagatac atgctgtggg gagataataa aatctaactg acggtcaaaa agattatcga
    16141 taacaccgtt ttcgctgaac tcaactggct gggcggtcac gccttcatac ttatctccga
    16201 gactaatcaa ctgatcgaaa ataattgtcg gataagtatt atctatgcca atcacaattt
    16261 ctcgtgaacg acgccctgag ttatggattt cattatcaat cgcagaaagt cgttgataaa
    16321 ccgggaacag tttttgatac agttcctgac cggctttatt gaggctgata ttattatctt
    16381 tgcgggtaaa tagcgtatag ccaatttgtt cttccagcgc ggcaatgctt ttaccaaatg
    16441 gcgaagccgt catatggatt ttttctgccg cccgggcaat attattggtt tgcgctaata
    16501 aaatgaaatt gcgcatcttt ttcgaaataa aaatgtccat cccccctccc cgcatccttg
    16561 tccgcttaac cgtttcagtc agcctatcct tgatgaaacc gcgagcaaag ataggtgatt
    16621 acgtcatggt tttacagaaa attacagaaa aaggaggcaa tatcgggtaa aggcattagc
    16681 ccgacgaata cgtcgggcta caagtattat tgtgctgcag gtgttttagc gggttgttga
    16741 tccacaggtt ctaactggaa gaccacatcg acctgatcat caaactgaat agcggcctgc
    16801 tcgtaagttt cctgggcaga caccggcgcg gcatcggctt tcatcatccg caccattggg
    16861 ctgggctgat agttggaaac atggtagcgc acgctatata ccggccccag tttacgatga
    16921 aagccgttcg ccagttcctg cgcctgatga atcgcgttat caatcgctgc cttacgcgct
    16981 ttgtctttat aggcatccgg ctgcgccacg cccagcgaca cagaacgaat ttcgttaaga
    17041 cccgccttca gcgcgccatc cagcaaggaa ttcagtttgt ctaactgacg gagcgtgact
    17101 tccaccgttc tcacagcgcg gtagcctttc aggatacttt taccatcctg ataatcataa
    17161 tctggctggg tgcgtaaatt cgctgagctg atatcttttt tcgcgatctg attgagttca
    17221 aggaaggaaa tgtattgtgc gacgcgctca tctgcctgtt tcttggctgt agcggcatcc
    17281 ttcgcggcca cgttaacttc aatcgcaaga gtggcaatgt ctggcaccgc atccacactt
    17341 gccgtaccgg aggtgacaat atgcggtcca tccggcaatt cgttagcctg cgctgccatc
    17401 ccgctaatac ccattaatgc cgccagggcg ataactttga acttcacgtt agtctctccg
    17461 tgttaattga cttgcccaaa attcaggacc tctggaggca agcttagcgc ctctgtttta
    17521 tttttccatc agatagcgcc taactgaaca aggcttgtgc atgagcaata ccgtctctcg
    17581 ccagctgcaa ggctataaac cacatgacac atcccactac cagattgata atgcgctgtg
    17641 attttgccgt gcgcaggcgc ggtgccagcc aggctgcgag aatagccaga ccaaagaacc
    17701 acaggaaaga ggcgctaatt gtcccgagcg caaaccaacg ttttggttcg acgtcaagct
    17761 gcccgccaag gctgcccagt acaacaaaag tatccaggta aacatgcgga ttcagccagg
    17821 tcactgccaa catggtggcg ataattttcc atctgccttg cttcaggact tcggcactag
    17881 ctaactcaat attactactc atcgctgttt taaaagcgcc aaaaccatac cacagcaaga
    17941 agactacgcc gccccaggtg accagcgcca gcaaccacgg cgactgcatc aataacgcgc
    18001 taccaccaaa aatccccgcg caaatcagga ccaaatcgct gatagcgcaa agtaaggcaa
    18061 tcataatgtg gtactgacga cgaatgccct gattcatcac aaaagcattt tgtgggccga
    18121 gcggcagaat catagctgcc ccaagagcaa gaccttgaaa gtaataagaa aacacgttgg
    18181 atattccgaa ttaagtatct gatgcagaga ctatatcgcg ggattgttat tagcggaaat
    18241 tgataatatt aattggcaat cagaataact aataagagag aggccagaga gtattatctg
    18301 gcctcagcgt tgattacgca gctttgtctt ctttcacccg cttaaagttc acatccattt
    18361 gcgggtacgg gaagctgata ccggcggcat caaattcacg tttaatacgc tccagcacat
    18421 cccagtacac gttttgcaga tcgccgctgt tgctccagac gcggaccacg aaattaatcg
    18481 acgatgcacc aagttcgttc aggcgcacag tcatttcgcg atctttcaaa atgcgatctt
    18541 cagactggat aatattggtc aggatctgct taacctgatc gatatcggaa tcatacgcca
    18601 cgccaataat aaattcgtta cggcgaactg gctcgcggga gaagttaata atatttccgg
    18661 caataatttt accgttcgga ataacgataa ttttaccgtc tgcagtacgc atggtggtgg
    18721 agaaaatctg cacactcagc acagtaccgg ctacgccgcc gaggtcaaca tattctccgg
    18781 cacggaacgg gcggaacatg acaagtaaca cgccagcggc caggttagaa agtgacccct
    18841 gcaaagccag accaacagct aagcctgcgg caccgagtac agcaatgact gacgctgttt
    18901 gtacacccac gcgtcccagg gctgcgatta gcgtaaaggc gataataccg taacggacta
    18961 atgcagaaag aaaatcagca acagtggcat cgattttacg ggagatcatc aggcgattca
    19021 ccgcgttgga aatcatccgc gcgataatca aaccaacgat gatgatcgcg agtgccgcca
    19081 cgatgtttac tgcataactt agcagcagcg cctggttagc taccagccag cttcccgcgc
    19141 cgtttatgct atcgacaaca ttcaaatctt ccattcaata ttccttttca actgactcaa
    19201 aatggagaaa tacactccgc cgccttatga cgggcagtct gacaagggta aacaataaag
    19261 cataattttg ccaaatagat cacagatttc tcatctgaat caccttgctt ttggcgaaaa
    19321 aatccccgca gagcggggct cagacagata tcaatgtgcg ctttgtcatg ccggatgcgg
    19381 cgtgaacgcc ttatccggcc tacaaaaacc tgcaaattca atatattgca ggatacgtgt
    19441 aggcctgata agcgtagcgc atcaggcaat tttgcttttg gcgaaaaaaa gacccgcaga
    19501 gcgggccttg agataagcag aaaggaatga cttacagaac gtcgatcgcg ttcagttcct
    19561 ggaatgcttt ttccagacga gcgatcatcg aagtctgacc ggcacgcagc catacgcgcg
    19621 gatcgtagta tttcttgttc ggctgatctt cgcctttcgg gttacccagc tgaccctgca
    19681 gataagcttc gttcgctttg tagtagttca gaacgccttc ccaggttgcc cattgggtat
    19741 cggtatcgat gttcattttc actacgccgt agcttacgga gtctttgatt tcctgagcag
    19801 tagaaccgga accaccgtgg aatacgaagt tcaggctgtt gtgcggcagg ttgtgtttct
    19861 tagaaacata ttcctgagaa tcacgcagga tggtcggagt cagaaccacg ttacccggct
    19921 tgtaaacacc gtgtacgtta ccgaaggacg cagcgatggt gaaacgcggg ctgattttgc
    19981 tcagctcggt gtatgcgtaa tcaacgtctt ccggctgggt gtacagtgca gaagcgtcca
    20041 tgtggctgtt gtccacgccg tcttcttcac caccggtgca acccagttcg atttccagag
    20101 tcatgccgat tttggacata cgctccaggt atttagagca gatttcgatg ttctcttgca
    20161 gagattcttc agacaggtcg atcatgtgag aagagaacag cggcttaccg gtagctgcga
    20221 agtgtttttc acccgcgtcc agcagaccgt cgatccacgg cagcagtttc ttcgcgcagt
    20281 ggtcagtgtg caggataacc ggaacaccat aatgttcagc catctggtga acgtgatgcg
    20341 caccagagat cgcgcccagg atagcagcac cctgcggaac gtcagatttc acgcctttac
    20401 cagcgataaa ggaagcacca ccgttggaga actgaacgat aaccggcgct ttaactttag
    20461 cagcagtttc cagtacggcg ttgatggagt cagtaccgac gcagtttact gctggcagtg
    20521 cgaagttgtt ttcttttgct acctggaaaa ctttctgtac gtcatcacca gtgattacgc
    20581 caggttttac gaaatcaaaa atcttagaca tgtctcttgt cctgtatcgt cgggccttat
    20641 actcatcata cttcaagttg catgtgctgc gtctgcgttc gctcacccca gtcacttact
    20701 tatgtaagct cctggggatt cactctcttg tcgccttcct gcaactcgaa ttatttagag
    20761 tatgaaaaaa tgcgtgctct aaaagcgcgc tgaaacaagg gcaggtttcc ctgccctgtg
    20821 attttttact tcttagcgcg ctcttcgagc atcgctactg caggcagtac tttaccttcc
    20881 acgaattcga ggaatgcgcc gccgccagtg gagatgtagg agattttgtc agcaatgccg
    20941 aacaggtcga ttgctgccag agtgtcgccg ccgccagcga tggagaacgc ttcgctgtct
    21001 gcgatagcgt tagccacgat ttcagtacct ttgcggaagt tcgggaattc gaacacgcca
    21061 accggaccgt tccacagaat ggttttcgca ttcttcagga tttcagccag ttcctgagcg
    21121 gaagcatcac cgatatccag gatctgctcg tcagctttca catcgttaac agatttcagg
    21181 gtagccggtg cagtttcaga gaactcggtt gctacgcgaa catcagacgg aaccgggatg
    21241 ttgcaggtgg tcagcagacg tttagcttcg tcaaccaggt cagcttcgta cagggattta
    21301 cccacatcgt ggccttgtgc cgcgataaag gtgttagcga taccaccacc aacaatcagc
    21361 tggtcagcga ttttagacaa ggagtccaga acggtcagtt tggtagatac tttagaacca
    21421 ccaacgatag ccaccatcgg gcgagctggt tctttcagtg ctttacccag cgcgtccagt
    21481 tcagctgcca gcagcgggcc tgcgcacgca acgtcagcga atttaccgat accgtgagta
    21541 gaagcctgcg cgcggtgagc agtaccgaat gcgtccatta cgaacacgtc acacagtgca
    21601 gcgtattttt tggacagggt ttcgtcgtct ttcttctcgc ctttgttgaa gcgaacgttt
    21661 tccagaacaa ccagttcacc ttcagcaacg tcaacgccgt cgaggtaatc tttaaccagg
    21721 cgaaccgggt tagacagttt gtctttcagg tagttaacaa ccggcagcag agagaattct
    21781 tcgttgtact cgccttcggt aggacgaccc aggtgggaag ttaccatcac ttttgcgcct
    21841 tgtttcaggg ccagttcgat ggtcggcaga gaagcacgga tacgcgcgtc gctggttact
    21901 ttcccgtctt ttactggtac gttcagatcc gcacggataa atacacgttt cccagcaaga
    21961 tccagatcgg tcatcttaat tacagacatg gtgaatcctc tcgttgattc taaaagtttt
    22021 gcagacgctg cttgcgtctt acctgaaagc aacagtagcc atagctaacg tcgtgtcgag
    22081 cattcggtta gcaaagcccc attcgttatc gcaccagacc aacgttttga tcaggtgtgc
    22141 gccactgacc cgggtttggg tgccatcgac aatggcactg tgcggatcgt ggttaaaatc
    22201 tacagagacc aacggcaatt ccgtatagtc aactatacca tgaaatgcac cttgtgctgc
    22261 tttttgcagc aacaggttga cttcattggc ttttacaggt ttcttcaccg tcacgcttaa
    22321 atcgattgcc gtcacattta tggttggcac acgtaccgca atcgcttcaa agcgatcgtt
    22381 aaattgcgga aaaaatcgtg tgataccggc ggccagttta gtatcgaccg gaatgatcga
    22441 ctggctggct gcccgggtgc gacgcaggtc aggatggtat gcatcaataa cctgttgatc
    22501 gtgcatggcg gagtgaattg tggtcacagt gccggactca ataccgtacg catcatctaa
    22561 caatttgatg acgggaatta tgcaattcgt ggtacaggaa gcgttagaaa cgatgcggtg
    22621 ttccgcacga agttgatcct gattgacgcc gtaaacaacg gtcgcgtcga gatcgttact
    22681 gccaggatgt gaaaagagca cttttttggc cccggcggca atatgcgctt cgccatgctc
    22741 gcgggagcca tatacgccgg tgcagtcgag gactacatca acgccaagtt cacgccaggg
    22801 gagcgattgc agtgaacgtt catgcaatac gcggatggcg tcatcaccaa caaaaagttg
    22861 atcgcgttcc tgtcgtactt cccatgcaaa acggccatgg ctggtgtcat atttcaacaa
    22921 atgcgccatg cccgcagcat ccgccagttc gttgattgcc accacggtaa tttccgcccg
    22981 gcgtccggat tcatacaaag cacgaaccac attacgcccg atgcgaccga agccatttat
    23041 cgctacgcgt acggtcatag atctcctgca aggttttccc tgagcaaatt tgccagacag
    23101 agtaatccag caaatcgtcc ggggaaacct tacctgtcgc aaactgcgac tgattggtta
    23161 attgtcgaac atttaatcga ctgaaacgct tcagctagga taagcgaaac gtggaataaa
    23221 aggaatgatt gtccagtcaa agaaagcaat tatctgactt acgtcacgtt ttcagccccg
    23281 cccctgtgga atttattcca ctttgcagaa tcacgaataa gcgtagcaaa atcttttcgt
    23341 tgtgccgtca caaaccctga cttatatcag aatatgactg atgcagcgtc gatattatcc
    23401 gcaatacgtc cgagtttcgg agtactccaa tgatgacatt gccctaccca tattcttcct
    23461 ccgcacgctt ttacgcactg cgcctcctcc ccgggcagga agttttttcc cagctacacg
    23521 cttttgcaca gcagcatcaa cttcatgccg cctggattgc aggctgtacc ggtagcctga
    23581 ctgacgtcgc cttgcgctat gccgggcaag aaggcaccac gctcctgaac ggtacattcg
    23641 aagtgatctc actaaacggc acgctggaac agagtggtga gcatcttcat ctctgcgttt
    23701 ccgatccaca cggcacgatg cttggcggtc atatgatgcc cggctgtacc gtgcggacca
    23761 cgcttgaact ggtgatcggt agccttgagg aactggcgtt tagccgccaa ccttgcgcgc
    23821 tttctggtta cgacgagttg catatttccc ccgttaaata actactttca aagaggtttt
    23881 cttatggcgc gtagccactt ttccagtcag gcactggtgt tgatcgttat atctatcgcc
    23941 atcaatatga tcggcgggca actggccagc atggtcaaat tacctatatt tctcgactcc
    24001 atcggcacac ttattagcgc ggtactgctt ggtccggtga tcggaatgct gaccggctta
    24061 ctgaccaatt tactctgggg attgctgacc aacccgatcg ccgccgcatt tgcacccgtc
    24121 gcgatggtca tcggcctggt cgccggttgc ctggcgcgag agggttggtt tcgcaccctg
    24181 ccaaaagtcg ttgtcagcgg cgtgattatt acgcttgcgg taacggtcgt cgctgtaccg
    24241 ctgcgtactg cgctgttcgg cggtgtcacc ggtagcggag ccgatttgtt cgttgcctgg
    24301 atgcactcga tggggcaaaa cctggtggag tcggtggcga ttacggtgat aggcgcgaac
    24361 ctggtcgata aaattcttac agcggtgatt gtctggcttt tactgcgcca gctgccgatt
    24421 cgcaccacgc gccattttcc ggcaatggct gccgtgcgct aaatgcatcc atttacctca
    24481 ttaacactgt gggcattggc ggcctgcacc acgcttatcc tgcccgcgca gaccattttg
    24541 ccgatctata gcgcggcgac ctttttctgc cttattgcgc taaaggccac gcagcggcgg
    24601 gcaaaatatg tcgtctggct gatgttctcg ctgggagccg ggctgtggct ggtacacggt
    24661 ggctggctac cagaatggct aagcggcacg ccacgctcgc cagaacggtg gtcgcatgct
    24721 attacgctct ggttgcggat tctggcgatt gtttcaacct cacagttgtg gatgcagtac
    24781 gtccccgtgc aatgttttat tcgcgctctg tttgcttctc gcctgccgcc aggcgtcgct
    24841 tatctgtttg ccgggccgtt attggtcgtt gagcagttaa aacggcaatt agcgattatt
    24901 catgaagcgc agcgtgcccg cggcgtgccg ctggatgagg gctggtatca gcgtttacgt
    24961 gcgatgcccg cgttaattat tccgctaaca cacaatgcgc ttaacgatct ggctgtccgt
    25021 ggtgcggcgc tggatatgcg ggcatttcgg atccacaacc gacgaacaac gttgtgggca
    25081 ccagccgata gcaccctaca acgcgtagcg cgttacacca tgatcttgct gatgctgaca
    25141 gaattcggag cgtggatatg gttacgttag aacagttccg ttattgcccg acacactcaa
    25201 cgcatcctcc gttctgttat gactttcact atgtcaaacc ggggatggtc gccatctttg
    25261 gtgacaacgg cagtggcaaa agtacgctgg cacagctgat ggcgggctgg tatccggact
    25321 ttctgccagg agagataaca ggcacgggga cgctcctcgg tacgcccatt gggcatctcc
    25381 cgcttaatga acaatccgcc accatccagc tcgtccagca atcgccctat ttgcagctct
    25441 ccggctgtac ctttagcgtg gaagaagaag ttgcctttgg gccagaaaat ctgtgtcttg
    25501 ctgaagcaga gattatggcg cggattgatg ccgccctgac tctgaccgag tgccagccgt
    25561 tgcgccatcg tcacccggca acactctccg gtggagagac acagcgcgtg gtcatcgcct
    25621 gcgccatcgc gatgcagcca aagctcttaa ttcttgatga agcctttagc cgcctgactc
    25681 cgcaggccag cgaaatgctg ttgcagcgct tacaacactg ggcgtttgaa cgtggttcgc
    25741 tgattattct ttttgaacgt catcgcacac ctttcctgaa ctactgccag caagcgtggc
    25801 aattgcaaaa cggagcgctc cagccactat gttaacgtta aataagatat cttatcgctg
    25861 gcctggcgcg gcaacggatt gtctttgcga catttctctg caactcaaac aaggtgaatg
    25921 gttggcgctg accggcgaca atggcgcggg aaaatcgacg ctgttacgag taatggcagg
    25981 tcttcttacg cccacagccg gtacggttat gctgcaacaa caggcgatga aaaacctgaa
    26041 aaaccgccag cgcgcggcaa aggttggcgt actgtttcag gaagcggaaa accaactttt
    26101 tcacagcacc gtcgctgacg agatcgcatt tggtttgaaa ctgcaaaaat gcccggcgga
    26161 tgaaatcact caacgtaccc atgctgccct gcaatgttgt cagttagcag acactgccaa
    26221 ctcgcacccg ttggatctgc atagtgcgca gcgacggatg gtcgccgtcg cctgcctgga
    26281 agcactctct ccgccactat tgctgctgga cgagccgagt cgggattttg acgaaaattg
    26341 gttgagcgtg tttgaaagct ggctggagaa gtgcggccag cgaggcacga gcgtcgtagc
    26401 aatcagtcat gacgccgcat ttacccggcg tcatttctct cgtgtggtgc gactggaaga
    26461 tggtctaatc aggaacgtta atccaccaga cgatattcac ccgtagcggt catcattaag
    26521 gtcaaatttg caggcagact ctcttccagc ccccggcgaa cattcggccc gtcggtacgg
    26581 tcataaaaag cttctgcgtc tgccagcgat acccctcccg ccagcttgcg ccctaccagt
    26641 cgttcccgca acgcagtagc tggcgcgttg ataaagatcg agaaatcaca aaattgcgca
    26701 agctggcacc acttctcgtc gtccagcaac aaccagtttc cttcgacgat gacgagtggt
    26761 gcggtaacgt gcaacgcatc ttcaacagga tcatgctttt gtcgatcata ctgcggccac
    26821 gtacaatccc cttccactac ctggcacaga ttttccgcca gtttcgcaac gtcaaatgtc
    26881 tctggtgcgc ctttgaaggg gcgcaattga tgcgcatcca gccagctatt gtaatggtga
    26941 aaaccatcca tcgggagcgt ctggatagcg ggtaattcag ggtcttgttg cgcgagatat
    27001 tcccagaagg tagttaaggt agatttaccc gtccccggcg gagcgcaaag aaaaaccacc
    27061 gtacgtcgct gcggacttac cgtctgtaag gccgccagca tacgcagcaa cggtttatgc
    27121 acgcgttcta tctcttcatc atggtactgc gcttgcacgt tcagcccatt caccgttaat
    27181 tcaattttca cggcgttaat tcctttaagg tggtcgttac tgccagcgtc agcgccgttt
    27241 tgaccattcc gctaaaacgc gcctctgacc agacggcgaa atcggcgaac ttcatcgctt
    27301 ttaacgcgtc atagagcgtc gcattgcagg ttatgctatt gacgttgctg ataaaatccc
    27361 aggtcatgtc atcggcaaat ccgggcgttt catcctgttc gttcaggtaa tgccagtact
    27421 gggaaaagtg cgtgatatcg gcatacagcc atttatccat tttccccagc gcataaatca
    27481 aacgcagcgc aacatcaata tcgtcaagcg ggccgctttg ggcgagtaac ggcttcactg
    27541 catattcgac gatctcttca tcgttatcga caaacagcga cggcagaaac tggcgtatcc
    27601 cctggagcaa aatagaatgc gcagttttca taaatgaaaa taaattgtcc tgtgcatcca
    27661 gttgctcaag cacatcatct tctgtcagcg tcgccattgg tcttccgata gatcaccatc
    27721 aatgcaggga gtctattata ttacacgttt ggtccgcgcc gccgatcagt aatgtctgcg
    27781 tccgcacccc attcgccgtc tgttggatac cgttcaccaa ctcgccgccc gtcacgcccg
    27841 tagcgctaaa gaggatatcg ttccccctca ccagttcatc gagcgagtac acacggttaa
    27901 cgtcaacgcc cattgcctta caacgcttgc gctcctgctc tgcaatctgc cgattttccg
    27961 tgtaatcccc tttagcctga caaaagtcaa tcagctcggc ctgcatatcc ccacctaatg
    28021 ctttaacggc acaggcagaa atcacgcctt ctggcgcacc gccgatggtg tacatcacgt
    28081 cgtaaggatt atcctgccag caggtcagca cgctggcggc gacatcgcca tccggcagag
    28141 cgaaaacctt cacgcccagc tgtgttgctt cttcaatcgc ggcactcagg cgcggtttat
    28201 ccagcgtaac catgcgcagc ttgtccagcg gtttacctaa cgcccgcgcc acgttacgca
    28261 agttgtccgc cagaggcaaa gacagatcaa tcgccccggc agccagtcga ttaacaacca
    28321 gctttttcat atacatatcg ggcgcatgta acaggctatc tcgaggggcg aaagccatga
    28381 ccgccagcgc attgctctgc cccatcgcta ccatccgcgt gccttcaatc gggtcaaccg
    28441 cgatatcgac ttctggccca tcgcctttgc ccacctcttc gccaatccac agcatcggcg
    28501 catggtcaat ttctccttcg ccgataacca ctcgtccacg aaaagcgacg tcgtttaatg
    28561 cctggcgcat cgcggtgacc gccaggccat caattttatt tttgtcgcca catccggttt
    28621 gcggccaggc agccaacgct gcctgttccg taacgcgaaa taatggccac gccagggaca
    28681 tcattgcgcg gcctccccga tatcaacgcc aaattgtgcc agcagatatt tctcggcctg
    28741 ttcattccag atgccgtgcg tttcctcaac cagccgcgcc aactctttaa acagcggatc
    28801 ggttttgcct ttttcggcaa aatcggcaat ggcagtgagc ggcatggtca cgccgttata
    28861 aatgagtttt ttaccgccag ggatatccgg cagattgagc acggtatctg gcaccgcatc
    28921 cagcccaccg atatgcgtga ccataaagga cggctgcaac tgccccgtgg cgcttaacgc
    28981 aatcgcctct ttcatgtcgt ccgttgaacc gccggatgta ccgactacgt gcgtgctatt
    29041 gtaatggacg ttgtagaaat taaacggcac tttgaagttt ttatccgtcg gcccggcaaa
    29101 gaagttcaga cagccatctt ccgccagtaa ttcgtcagcc atctcaacga cagcaggcac
    29161 cgccgcataa acaaaaatgt catcgaaccc gacatctcct gtcagcgccc gcagcgtctg
    29221 gaccggatcg ctcatccctt tggtattcac atacaccagc tcaatgcctt tactggccgc
    29281 cagatccacc ggcagcagct tctgcacctg cgccaggcgt ttgtcgtcga tatcgaccac
    29341 caccacccgt gacggttgta tgccaccgtt aatggcgtaa tcgatagcgc caatgcccat
    29401 cggacccgca cacgccagca gtgcaatatt gccgccaggt ttgacgccca tgcgatgctc
    29461 ataaacatat tgcgtggtgt gataattggc atgataagca ccaataatgc agcacatagg
    29521 ctctgccagc gacgccgcag caaaataaga gccgtgatac ggtaatacgc agcccaaatt
    29581 aatggcgatt tcgggaataa tcatataggt ggcgttaccg ccaaaatatt cgtagctgta
    29641 gcctgctgaa tatccgcttg gtaaccccat cgccggttgc aatacaaaac gctggccttt
    29701 tttatatttg ccagtgagat ttttacccac ttcgacaata accccggcac attcatgccc
    29761 ggtaatgacc ggatgatttt ctaaatcgtc gggtacgcgt ttatgttcac taccgagtaa
    29821 cgccgctttc caggtcgata aacagacgct gtcagaaatt acactcacta acagttcatt
    29881 atcggtaatt tctggcagtt caaattcgcg cagacggaca tcccgcttgc cataaatagc
    29941 agcaactttg gttttcattt ttacctctgt aatcagtgtt tatgttcggc ggttaattga
    30001 ttaaataaag tgtcgagttt tggatcgcca atatagttat taatcaatat cagcggttta
    30061 tccgtcacac gtttcacgcg cccttccaga ctggcgtgag tgacgacgat atcggcatcc
    30121 gcaggcacgt tttctatcgc gtaatgtttt acttctattg ccagccccgc tttttccagg
    30181 cgtttacgga aggtggtcgc gcccatcgca ctggagccca tccccgcatc gcaaacaaag
    30241 gcgatacgct taaccctgct taacgagaat gcaccctctt gtttcatcgc cttaaccgca
    30301 ttggctgact gagcaaactc atcttcgctc tccgtttcca ccgttttttc catcttcagt
    30361 atcagcgaag taatagcaaa ggacaccagg gtacctaccg taacaccggc aattgtcgcc
    30421 aggaacgagc ctttcggcgt cagtgccagg taagcaaaga tagaccccgg acttgggcca
    30481 gccaccagac caccgtccag taagttaaac atccaggtac cagacatacc gcccgcaatc
    30541 atggcaataa tagtcagcgg cttcatcagc acatatggga aatacagttc gtggatccca
    30601 ccgaggaagt gaataatcat cgccccgggc gcagaacgtt tactcatccc tttaccaaac
    30661 aaggtaaacg ccagcagcag ccccaggccc ggacctgggt tagaggccac cataaagaag
    30721 atggatttac cgttaaccga agcctgttgc attcccagcg gataatagac gccctgatcg
    30781 atcgcattat taaggaaaag cactttcgcc ggttcattga tgactgacag cagaggcaga
    30841 taacccgcat gtaccagcgc ctcgatacac tctttgacga aagtattggc aattaacacc
    30901 gccgggccga tcacttcaaa acccagcaga caaaggagca tccccgcgat acctaatgag
    30961 aagttattga taaccatctc aaaaccggca ggtatgcgtt tttccagtgc cttatcgaca
    31021 tattttatga ccaacccacc gagcggcccc atgatcattg agccaaggaa catcgggatc
    31081 tctgcgccaa cgatcacacc aatagtacct attccgccca tgaccgcccc gcgtttaccg
    31141 ccgaccagat gaccacctgt agaaccaatc atcacgggca ataaataggt aatcatcggg
    31201 ccgacaattt tggcgaaatg ttcgttaggc agccaaccgg tgggaataaa taacgcagta
    31261 ataaaacccc aggcaataaa agcaccaata ttggggatga ccattgcagt caaaaagccc
    31321 ccaaaagcct ggacctttgc acgagcagac ttgttttcca taatattatc ctgtagagga
    31381 gagagtaatt atccgcggct gataatgtcc gctaattgtt tttctgatga tgctgttaag
    31441 agttgttcga gtatttcttc ttcgcacagt aattcactta acgcctgaat agcgccaatg
    31501 tgcgaatcgg catcggcagc agagagtccg atgagtaatt taattggttc gtcattcccc
    31561 ggaaaataaa caccttgttc aagtaatgtc aaagacatcc cggttttaag cgccccacat
    31621 tccggtcgcg catgaggcat tgccacgcct ggtgcgagaa tataataagg gccattgtta
    31681 atggtggaat ctttaattgc ctgaatgtaa ttctcgctga tatagttttt atccagcaat
    31741 gataccatcg agaaatcgat agcttcctgc caatcttttg ctgaatgtat aactgagatt
    31801 gatgattctg gaaaataatc actaagccgc ataaattatc cttattttat ttttagggta
    31861 ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg aaagcaattg agcagggaaa
    31921 ccgatgtttt gaaaacagtg aattattaat ttcttatata acattgagtt atagatataa
    31981 caacaaatgt caatacgcat atcgtgatgc atattacata attgatattt attgcatata
    32041 aatatttgtg atctacaaca cgctttatct attgcttgtc cgcaaacgga catatcaagg
    32101 taataaaaaa ggtcgccgaa gcgacctttt tacccgaaat gctaattaca gcagttcttt
    32161 tgctttcgca acaacgttat caacagtgaa gccgaactct tcaaacagca gctctgccgg
    32221 agcagattca ccgaaggtgg tcataccgac gatagcaccg ttcaggccaa catacttgta
    32281 ccagtagtca gcaatacccg cttctacagc aacgcgtgca gtaaccgctt tcggcagtac
    32341 ggattcacgg taagcagcat cctgcttgtc aaatgcgtcg gtagacggca tggacaccac
    32401 gcgcgctttc acgccttcgg cagtcagttt ttcgtaggca gcaacagcca gttcaacttc
    32461 tgaaccggta gcgatgaaaa tcagttccgg ctgaccggcg cagtctttca gcacataacc
    32521 accgcgcgcg atgtttgcca gttgctcttc agttcgttcc tgctgcgcca ggttctgacg
    32581 ggagaggatc agtgcggtcg ggccgtcctg acgctcaaca ccgtatttcc acgcgaccgc
    32641 ggattcaacc tggtcacacg gacgccatgt agacatgttc ggggttacgc gcagagaagc
    32701 gacctgctct accggctggt gagtcgggcc atcttcgccc agaccgatgg agtcgtgggt
    32761 gtaaaccatc acctgacgct gtttcatcag cgcagccata cgtacggcgt tacgtgcgta
    32821 ttccacgaac atcaggaagg tggaggtgta cggcaggaaa ccaccgtgca gggagatacc
    32881 gttagcaatc gcggtcatac cgaactcgcg aacaccgtag tggatgtagt tacccgcagc
    32941 atcttcgttg attgctttag aaccagacca cagggtcagg ttagacggtg ccaggtcagc
    33001 agaaccgccg aggaattccg gcaacagcgg accgaaagct tcgatagcat tctgagacgc
    33061 tttacggctg gcgattttcg ccgggttagc ctgcagttta gcaatgaact cttttgcttt
    33121 cgcgtcgaag tcagacggca tttcgccttt catacggcgg gtaaattcag cggcttcctg
    33181 cggataagct ttcgcgtaag cagcgaattt ctcgttccat gcggattctt tcgcctggcc
    33241 tgcttctttc gcatcccact gagcatagat ttcagacggg atttcgaacg gtgcatattt
    33301 ccagcccagc tgttcgcggg tcagggcaat ttcagcgtcg cccagcggcg caccgtggga
    33361 gtcgtgagta ccggctttgt tcggggaacc gaaaccgatg atggttttgc acatcagcag
    33421 ggacggtttg tcagtcactg cgcgcgcttc ttctactgcg cgtttgatgg atgccgcgtc
    33481 atgaccgtcg atgtcgcgaa taacgtgcca gccgtaagct tcgaaacgca ttgcggtgtc
    33541 gtcggtgaac cagccttcaa cgtgaccatc aatagagata ccgttgtcat cgtagaacgc
    33601 aatcagttta cccagcttca gcgtacccgc cagagagcaa acttcgtggg agatgccttc
    33661 catcatgcag ccgtcgccca tgaaggcgta ggtgtagtgg tcgacaatgt cgtggcccgg
    33721 acggttaaac tgcgccgcca gcgttttttc tgcaatcgcc atacctactg cgttggcaat
    33781 accctgcccc agcggaccgg tggtggtttc cacaccagcg gtgtaaccca cttccgggtg
    33841 gcccggagtt ttagagtgca gctgacggaa gtttttcagt tcttccatcg gcagatcgta
    33901 accggtgagg tgcagcaggc tgtagatcag catggagccg tggccgttgg acagcacgaa
    33961 gcggtcacgg tcagcccagg acggattctg cgggttgtgt ttcaggaaat cacgccacag
    34021 gacttcggca atgtcagcca tacccatagg ggcacccggg tgaccggatt tggctttctg
    34081 tactgcgtcc atgctcagcg cacgaatagc attggcaagc tctttacgtg aggacatttt
    34141 gactccagat cggatgatga agggcacgcc cttaacgact tgacgacagc gcgttttggg
    34201 ctacgccgga aaatttgcca acaatttacc gcaagccgcg cgtcatgtac atggaacatc
    34261 cttttgccgc ttcagaaatc tctggatcat gctcgcatgt tgcgcaatct actcgcccgt
    34321 ccgctgcgct tttccttata ctgagactga gcgtcgattc acctgcaaac ggcgcatttt
    34381 tagaataatc ctgaccttgt gcggaagaga aaacatgaaa attcgcgcct tattggtagc
    34441 aatgagcgtg gcaacggtac tgactggttg ccagaatatg gactccaacg gactgctctc
    34501 atcaggagcg gaagcttttc aggcttacag tttgagtgat gcgcaggtga aaaccctgag
    34561 cgatcaggca tgtcaggaga tggacagcaa ggcgacgatt gcgccagcca atagcgaata
    34621 cgctaaacgt ctgacaacta ttgccaatgc gctaggcaac aatatcaacg gtcagccggt
    34681 gaattacaaa gtgtatatgg cgaaggatgt gaacgccttt gcaatggcta acggctgtat
    34741 ccgcgtctat agcgggctga tggatatgat gacggataac gaagtcgaag cggtgatcgg
    34801 tcacgaaatg gggcacgtgg cgttaggcca tgtgaaaaaa ggaatgcagg tggcacttgg
    34861 tacaaatgcc gtgcgagtag ctgcggcctc tgcgggcggg attgtcggaa gtttatctca
    34921 atcacaactt ggtgatctgg gcgagaaatt agtcaattcg caattctccc agcgccagga
    34981 agcagaagcc gatgattatt cttacgatct tctgcgccaa cgcggcatca gcccggcagg
    35041 tcttgccacc agctttgaaa aactggcaaa actggaagaa ggtcgccaaa gctcaatgtt
    35101 tgacgaccat cctgcatccg ccgaacgcgc ccagcatatt cgcgatcgta tgagcgcgga
    35161 tggggttaag taagtcagac ataccgatct acaacataat tgtaggtcgg taacgcctga
    35221 atccccgatc agagtagtga tttcacttta tttagcaaaa cggctacatc atccggtcgg
    35281 gtatatcccg tatcagggtc tataatcgag ctgtaaacat gtggcataat gcgcggcacc
    35341 cccgcctcca gacaagtttt cagaatcaca ctgaagttat ccaaatcaat cccgccagtc
    35401 ggctcaatca gcgtcatacc attgcgcgcc gccgtggtcg ccagcgcgta tagctccgga
    35461 agcgacctct cgccgcccat cggaaagaac ttcgccgcgt gtgcgcccat atctagcatc
    35521 attcgcaccg ccgtatcgca ggagacgcgg gcaggcgtac cctggctgct gctaacgccg
    35581 gtggaaatca gcacttcgcc cggcgtcccc gtcgggctaa ccagcgcgtt gacatgggtc
    35641 tgctcaccgc ccgtagccgc aagcgcgcct gccgcgaaac cacaaccggt aaaggtctgg
    35701 ttaacgtgtg ctggatgaat ctgcgccgca atcatcgcag ctttgtaata ctgcgccggg
    35761 tcacccgccc ccagacctac tgaaatggac ggtatttccg ccatccaacg tttaacttcc
    35821 tggatcccct catccaccga ggagaactgc gcggaaagca cgccaatcac tgcgtggcct
    35881 tcggcggcgt caaagatttc acgggcattg gcgatatctt tcgccagcac attaattgcc
    35941 acgcgattac gatagaaatt aatcttttgc atgatctgca atctccttca gtcgcgccac
    36001 gataagtgcc atttcccctt ccgccaccgt acgcggatca aggctaaaaa cgccctggtg
    36061 gaggttatac cgccgtgcat aaatcgcgat atcgccacca cgcagttgcg cttcgacgac
    36121 gcgagcgtca actcccaatt cctgcgcatt tactcgaata cgaatacgcc agattgcacg
    36181 cccggcttca tcctgctcaa tatcagccgt taacccgtga atcgcagaaa tcgcttcaac
    36241 cactggctgg agctgttccg cggtgatgac tgcctgcccc tggtgataat tctccagcgc
    36301 gtaaaccagc ccaaccatgt tctccttgcc gattttcatc gccctggcaa tgccgtgatg
    36361 ctgcgctttg caggctgcaa tccagatttt tttaccagtg ataaacccgg aggtcggcgc
    36421 gttaaaggct tttgcgccgc tgtagaccac catatccgct ccgctcacca cccaggcacg
    36481 taaatcctct tcggcggcgg catcgacaat cagcggcagg ttgtacatct gcgcgacatg
    36541 tgcgaagtcc tcgatgttca acattccctt ctgcacgcag tgatgcgact tcacatacag
    36601 cagcgcggcg gtcgcgtcgg taatggcact ctccagctgc cagagagccg ccaggttact
    36661 tgaacccacc tccacaacgc gcccgccacc taagcggatc gcgctgagga tcgatgcacc
    36721 gtaatcgacg ttatggccgc gcagcatcac cacttcattg gccatgccgg agctatccgg
    36781 catgagcgcc acccgggcct tgtcgccgcg tgtaatggcg gcggcgacag cgatggcgat
    36841 ccctgctgag gcacaggagg taacgtagct atcctccgcc ccggtaaagc gagagaccag
    36901 ctcgccggtt ttgtccacca gctggtcaat ctccacgaaa gaggatgccg cgcgcgcggt
    36961 ggcctgcatc acctccggcg cgacgctgga gacgccgaga atcgtcatct tgccgcaggc
    37021 gttaatcacc tgtttcagtc ccagttgttg atagatattc tgcgtcatgg cttacagcac
    37081 tcccagcagc gagcagacca cgctcaacgc cacaatcgac agcagaatcg tggtgtaacg
    37141 cggacccttt ttcaccaaat agaagtagat ggcgaacact gccgccagcg gcagcaggcc
    37201 gggcgcgatg gagtccagaa tctgctgtac caccacttca gagcctttca gtgcgctaat
    37261 ttttaaaggt gaggttatct tgacaaagct cgcggatagc gcgcccatca tgataagccc
    37321 cagtacgtta gcgccgtaga taagctcctt gatgcgccct ccctgcagca gactgataat
    37381 cgaatcgcgc cccagcgtat agcccttgtg caccatgccg tagctgattg ccagcgtaat
    37441 cgccgggtag agaatcagcg gcaaaatacc gcccatcgcg ctgccattgg cggcgaacgg
    37501 gataaaaata gcaatcaaca gcggcatcac cgccgcccag atgatggagt cgcccatccc
    37561 ggccagcggc cccatcaggc cggttttaat cccggtgata gaggcatcgt taatcgcctc
    37621 gccgcgggtc ttttgctctt ccatggcgat ggagatcccc tggatcaccg cgccaaacgt
    37681 ctgctctgag ttgaagaagt tcagatggcg ttttaacgcc tccacctgct cctccttttg
    37741 cggatagagc tttttgataa tcggcgtcat cgaggcgcag aaaatcaggc tctgtaaacg
    37801 ctcataggag ctggaaactt ccgcgcccag ccagtagata aaccacgctt tggtgatatc
    37861 cgctttggtc agcgccccgg actcccgcgc gcgctcaacc agttcatgct gcattacgtc
    37921 gtcgctcatt atgccgctcc ttcatttttc gccaggcctt taatcaggaa ggccacgcag
    37981 gtgccgaaaa tcgccatcgc catgatgtcc accttgaggt acagcaccgc gaagaatccg
    38041 ccgataaacc acggcagcag gctcttttta ccaatcacca taatggtgat ggcgaagccc
    38101 agcgccggga gaatcccgcc catgatttca aacgagtggg tcagccagtg cggcatcagc
    38161 ttgaggaaac tttccactac gttctggccg aagtagttgg cggcgaacac cactgggaaa
    38221 cgtagtacca gccccagcag cgccgggtag agaaaggcgc agcgcataat ggctgacata
    38281 ttggccgttt ccgcgtgttt atcggccata tgcacccagg ccgcatttag cgtgcgacgc
    38341 aactggtcga ggaatacgcc gataacgcca aatggaatcg ccagtgcaat cgccaggttc
    38401 gggtccatat tggccttaac ggcgatagga atggcgatac aggccgccag cgccgggtct
    38461 gacggcacat tgccgcctgg cgttgaggtc acacccagat agaccaactg catccccgcg
    38521 ccgataatca tcgccgcagg catattgccg agcagcaggc cgacaaagac cgccaccacc
    38581 actggctgta acagcatggc ggagaaggta tagccaaggc gcaaccgggc aaaccagtaa
    38641 tacagcccca tcaggcttgc aaaaagtaag gtatccataa tttgttatcc catgttcaga
    38701 gggtcagaat tttttcagga tatcgtccag cgactgcggt ttatcttccg ggatggtctg
    38761 gaaaacagcc ttaatgccgc gacttttcaa atcgttaaga atgccaacgt ctttttcatc
    38821 cagcgttatg ttctggaaca ccgctttacg gtttggcccg ccgcctaaac cgccaacctg
    38881 aatatcagcc acgtcaaagc cctgctgcac cgcttcctga atcgccgcca gcgacgggaa
    38941 cagcaccagc acgttgccgt cgcccagctg gttctctttc cacgcggccg cgaagctctg
    39001 gttgctaaag cagtccactt tgatattcgg cggcgcggcc atcaggtaga tatttttcat
    39061 aaacggatcg gcgtccagct cgtcgctgac caccgcaata cggttggcct gagactgccc
    39121 tacccacttc gtcaccacct gtccgtggat aagacggctg tcaatgcgac ataacacgat
    39181 gtttgccata cgattttcct tactgttgta attgacgaat atgggagacg acgtccaggc
    39241 aactgttgcg cccagcttcc accagatcgg ccacgcaggc ggtcagtggg ccatgttcgc
    39301 gcttgtccag cgcctccagc agcagcgagg cattaagccc ggagatcacc gccaccggat
    39361 aatcggcgct cagccgcgcc gccacgttag aggtggtgcc gccgataaaa tcggtcacga
    39421 taagcgaacc ttccggcatg gttttcacca cggcttcgac gcgctggtaa aactcgccca
    39481 gcgtatcgac gggcatcagc gcgatttccg tcacgccttc gatttcgccc atcaccatgc
    39541 gcagactgtt gcagagctgc agcccccagc cgccgtgagt tagcagcaga atttcaggta
    39601 acggatgaat aggtacagtt gtggtcaaaa tagcctcctt tgctccagtg tttacatttc
    39661 atcaggagca aaggacgtgc cagaatttgt gtcacgcaca gaggaaatga gattggatgg
    39721 ggcgttaagc cccgtaaacc aggggatcag ctgtagagca gttcgtagat ataaaaatat
    39781 tctgcgtccg ataaacggat agcgtaagcc tcttcaatcg gcaaaaagga ggatttgatg
    39841 acgctaaagg cacgagcgtc cagatcaagc cggttttcta gcgccatctg taacggtttg
    39901 cggttaatca cgatacgctc gaccatgcag cagcagtgga tcagaaaacg tagtgtcacc
    39961 tggcggctcg gtttcaggga gagcgtgttg gttaagtagt tcagaacatc ctcgatctct
    40021 ttcagaattc gctgcgggtt gagcaccgaa atatggttga tgatgctctc catggtcagc
    40081 gcgctgataa agcgcatggc gctgcgctcc atctccagtc ggcgctcgct gcttgagaga
    40141 tccggcgtca gcaggctgag caccagctcc ggcccttgct cggaaaacag ttcctccagc
    40201 gagataaacg gtatatccgg cagtcccggc tggaaggtgc cgacgatccc tgccagccgt
    40261 tcactggcat taagcgcctg ctgaatacgc tccaggctgc gcacctcgtt atagtcgaga
    40321 atcaccatac gcgtgtcctg cgacatcagc tcgccaaagc tctcctccag cacctttttg
    40381 atcttttccg ccgtccccat tccggttata catgagataa ccagcacttt gccaccgctc
    40441 tcttgctgcg gcgtgcagag ccgacaaggg atatttttac tctgcataag cacagccaat
    40501 tgcgatagat cgccagtttc gtagcttaaa tccagcccaa cctccagcag gctggtcaga
    40561 gtgatatttg gcaacaacag aacgtcaatc tggaacagct tgctgatggt gctgccaaaa
    40621 tggaccagcg atccgatatc caccatcagt atcagccgct gccagcaccg gtcctgaatc
    40681 atccgggtca gggtttccag ggtgtcatgc accgactgct caaatggcat atcaattgcg
    40741 tagaacagat cgcgctccag cacccggttc acatattgcg cctggctcgt tgcggtggtc
    40801 gcaccgtgag cgattagaat cacgccgcaa tccgggctgg tgtcgatgcg ctggcggtag
    40861 tggcgacact ctttcaggaa caggcacagc cagaccacct ccgtcgccgg gcactgaata
    40921 tgcaacaact cattgatttt gcggcacaga agcgtggcat tgtcatactc atctttacag
    40981 cgatccagaa tcaggctgga tgaatagagt tgaggtataa gccctcgccg cacatagcca
    41041 atcagcgcaa gaaagtgttt acgtaacggg ttcgccaggt tttctggcaa tgtaaatcct
    41101 aatacctgct ccacgtaacc gatcagcagc gtcacacgct cctcaatttg atcgccatag
    41161 cgcggtggat gcgccgcact atggcggcta taaagaccat attcaaaaat cgaactgagt
    41221 ttgtttttca ggatagccag cgtttctgcg ggcggcacgt tactgttgcg cagattgaca
    41281 tattcacgag tcaggaaact gtaaaacagg tcgctctctt ctatttccgc gctggtcgcc
    41341 aacgaatttt ttaacgcggg caacgtacgc gcatccacgt tcagacgatc tttaccgcca
    41401 aacaacgcat cgaccagcag acgctgctct ggcgtggtat taaacgacat ctccgccagt
    41461 cgtttatcta actctagcgt atcattacgc tcggtcattc ccgacgccca cgcctgcgcg
    41521 cacaagaact gaatatcact tttaagctgg ccgatattgc cttccagggg tttacttaac
    41581 aaccaatgca agagcgtttt atcaaggctt atggtacgtt caattttacg actttcacgc
    41641 tgaaagaagc ttacaatcaa ttcgagctgc tcctcgaccg aacgctggcg aatacccggt
    41701 aagtcaatac atacctggat acgccgctgg aaggtacgca atagtgccga gttgaccgac
    41761 tctgtagtgg cgcaaataag gcgcaccgaa attgaatatg ttggcccact tgaaccaagt
    41821 gggcggtatt cacctttatc gagaatagaa aagagcttct cttgtccttc gtacgggagg
    41881 cgatgcactt catccagaag taaataacct ccgtcagctt gctccaccaa tcccgtttta
    41941 ttctcacttg ccccggtaaa tgccccctta tggtgaccaa acagatgtga ggaaagcaac
    42001 tccggattat gagcatattc ggcacagtta aagtaaacca acggaggtgg cgaacctatg
    42061 gtgtgcttgc aagcgaagca atgcattaac tcagcaaaga atgttttgcc aacaccagat
    42121 gcaccggtta acagcacatg tagtccgtgg ggataaagca ccgctgcgcg gcctttttct
    42181 accgcatcac ggagactacg gtcgtatccg ataagtccgg aaaacggatc atcatctgta
    42241 tatttttctt tctttggcaa taaactagcc actgaatgaa ctatgcattc gcaatcatcc
    42301 agttttcgat taatgagcaa ttctagtgcg tgacgatgta gaaaaaaaac aggacgtccc
    42361 tgacttttta ccaccaggcc ttcattccat aattgattaa gatctttact aaccgagttg
    42421 cgtgccagcc caaggttaaa accaatcgcc tcagcagtaa atgctttctc cttcgctaaa
    42481 tcagacagtt ccagcccata cgtcagccgc tccagctcct tgtgaataac atcaatccgt
    42541 ctcatttctt taccgcttaa aaaacaatag ttgttcagaa catacactat ttgtaaacgt
    42601 aagaatgaga actatcggga agaattaatg ttccttgtgg caatctggtt tctgaatatt
    42661 cgtgttttaa atattactta tggtctgaaa caaccaggca ggtgaacaga gctaagaaaa
    42721 agtagcgtag aaatataaaa acatatatcg cactgatgaa tgtttcccgg catgactatg
    42781 cacaccggga aatatttagt aaattactcg ccctttttcg ccgcctgaat atacagcatc
    42841 tccagcgcca gcgtcgccgc agccagagca gtgatttccg actgatcgta tgccggagcc
    42901 acttccacta cgtccatccc aacgatgttg agatctttca ggccgcgtac cagtttaatg
    42961 gcgcgatcgg aggtcagacc gccaattacc ggcgtaccgg ttcccggcgc aaatgcagga
    43021 tccaggcagt cgatatcaaa ggtcaggtaa accggcatat cacccacaat ttgtttcacc
    43081 tgggcgataa cgtcatccac gctgcgatcg ttcacctggc aggcgtccag cacggtaaag
    43141 ccgttgtctt tatcaaattc ggtacgaata ccaatctgca cggaatgatt cggatcgatc
    43201 agaccttctt tcggcgcggt atagaacatg gtgccgtggt caaattcaca accgttcgcg
    43261 taggtatcgg tgtgggcgtc aaagtgtacc agcgccattt tgccgaaatg cttcgcatga
    43321 gcacgcagca gcggcagcgt aacaaagtgg tcaccaccga aggagagcat acgcttaccg
    43381 gcagccagca gcttctcggc gtgcgcctgc agcttctcgc tcatctcacg ggcatcgccg
    43441 aaagcatata ccagatcacc gcagtccacg acgttcagac gctcacgcat gtcgaaattc
    43501 cacgggaagc ggttgtgttc ccaggccaga ttcgtcgaaa cctgacggat agctgccgga
    43561 ccgtggcgtc cccccgcacg accagaagtg gccatatcga acggcacacc agtaatcacc
    43621 cagtcagcat cgctgtcata cggctggaag ttcatcggca ggcgtaaaaa accaaaggca
    43681 ttggaaacca gtgagttatc gtattgatga cctaaggtgc tcatggatgg actcctatta
    43741 caaagtcgat ataaaaaaac ccttccgcgt cgttaggccc gacgaggaag ggttggattt
    43801 gcgaaacaca agtttgtgct aattatcgcc ttaaatgggc gaggcatcaa gcgaggttgc
    43861 tatttccgac acggtggcgg cgcatagcca ttgttatctg ccggaaaggc ttgtccagag
    43921 cacggcggca gcgcgttttg cgcctggcct tgtaattcaa aggcggcaaa gcctattaca
    43981 ccggtattac gcacatcgcc ttgcgccgaa ttggctgcgt aggaatcagc aacgttggcg
    44041 aactgaaaag ccgcttcggt atgtttatct ttgcggaatc ctttaatcgc taaagaatct
    44101 ccggcattca caatatagcc gttgttattc agcgaacctt gcttgccgtt cagcacatcc
    44161 aggccatcaa cagtagcaac gatctcgtag ttggtgttac gactgtaatt acggacatat
    44221 aattgataat tcattccgac tctggcatgg acaatatagc gtggttcatt attggatgaa
    44281 tcataaacac gcgtaatagg tagagaatta aaattaccat cgcgaatagc atattccagt
    44341 tcgttaatac gaatgctata aacgcggtca tagccggaag gaacattagt cgaataacta
    44401 ataacgatca tctctgccgg atcgcgccag gctcgctcag cagatacgcc ctgaaccgat
    44461 gagtgcacat ccccacccca ggcagtgccg acattgcccg atgcactgct attatactgc
    44521 gcattactgg aacaaccggc cagcagcacg attaatgcca ccaacgcgct acgtactttc
    44581 cattttttca taaccgttcc ctctgcattt aattaatgca gcgattgtag ggagattatt
    44641 taataagaaa agcagcgaga ttatttttaa gaatatataa aaagaaagcg ggccgattta
    44701 cgtaaataaa tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataggtata
    44761 accgtacaaa cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc
    44821 ggttttcttc acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata
    44881 ttgcagcatg tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc
    44941 agggaacacg aacacgtcaa ccgcttcggt atcaccgaac aggttgtgca tattgccaag
    45001 gatctcctga tatgcgccga ccataaagaa accgagcatc ggcggattct ctggatcgta
    45061 ctccggcatt ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc
    45121 gtcagagtca caggtaatat ccagcagcac tgcgcgacgt tccggcactt gatccagccc
    45181 ttccagcggc agaaccggga acaactggtc gatcccccat gcatccggca ttgactggaa
    45241 cagcgagaaa ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg
    45301 acgatgcgca cggttttgcg gatccagctg cttttgcact tcatggcaca tgctcagata
    45361 gagttgttct gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg
    45421 aatgtcgtgc agatccatct ggctgtcgtg taaccattca cgcagagaac ggcgagttcc
    45481 cggttcgtgc atttcctgcc aggtttccca catgctttgc agcgcgcgcg gcgcatcttc
    45541 tgcaggcgcg gtcggcaccg tgtattcgtt acgttccacg ccgatgatat tagataccag
    45601 cacggtgtga tgcgcagtca ccgcacgacc cgattcggtg attaccgtcg gatgcggcag
    45661 accgttttct tcacacgcat caccaatcgc ccagatgatg ttgttggcat attcattgag
    45721 gccgtagttc accgaacagt cggactgcga acgggtgcct tcatagtcca cgcccagacc
    45781 gccgccgacg tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg
    45841 cgcggattca cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg
    45901 gaagtgcagc agttgcaggc tgtcgagacg cccggcttca cgcagggttt caacaagttg
    45961 cagtacctga gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt
    46021 acccgagccc tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg
    46081 ttctgcttca tccagcacaa tggcgatttc tgacatcttc tcaatgacca ggtagacctt
    46141 gtggcccatc ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt
    46201 gcagacgatg acgctacggg tcatgccagc atgtgccagc accgccatca actctgcttt
    46261 cgagcctgct tccagtccca gcggttcgcc cgaatgaatc agggactcaa tcacgcggcg
    46321 gtgctggtta actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc
    46381 ccgcgcacgt ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa
    46441 acagaacagt gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag
    46501 atcgacgcga gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt
    46561 aacgtcatag tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc
    46621 ttcctgggag ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga
    46681 cgaaggcaaa cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt
    46741 gtaaaacagt taacgactat cgcagcctgg aactgcgata acaacccata agcacatgga
    46801 ttttccagca gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta
    46861 ttaagcacat aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt
    46921 tttacaagag cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa
    46981 gcgccgagat gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac
    47041 gaatcttcac cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat
    47101 cacctccacg gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc
    47161 tggaagtggc aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt
    47221 tataccccac aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag
    47281 gaaaaatttc cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc
    47341 gggtgaagaa ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac
    47401 tcagactgcc agtgttttta acttgcagag tcgtggtagg atccgctacc acagaaaatc
    47461 cacacaacag tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc
    47521 tttttacgtc cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatttctg
    47581 atgccgtttt agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct
    47641 acgtaaaaac cggcatggtt ttagttggcg gcgaaatcac cactagcgcc tgggtagaca
    47701 tcgaagagat cacccgtaac accgttcgcg aaattggcta tgtacattcc gacatgggct
    47761 ttgacgctaa ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc
    47821 agggcgttga ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg
    47881 gctacgcaac taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc
    47941 tggtacagcg tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg
    48001 cgaaaagcca ggtgactttc cagtatgacg acggcaaaat cgttggtatc gatgctgtcg
    48061 tgctttccac tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg
    48121 aagagatcat caagccgatt ctgcccgctg aatggctgac ttctgccacc aaattcttca
    48181 tcaacccgac cggtcgtttt gttatcggtg gcccgatggg tgactgcggt ctgactggtc
    48241 gtaaaattat cgttgatacc tacggcggca tggcacgtca cggtggcgga gcattctctg
    48301 gtaaagatcc atcaaaagtg gaccgttccg ctgcgtacgc agcacgttat gttgcaaaaa
    48361 acatcgttgc tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg
    48421 tggctgaacc gacctccatc atggtagaaa ctttcggtac cgagaaagtg ccttctgaac
    48481 aactgactct gctggtacgt gagttcttcg acctgcgccc atacggtctg attcagatgc
    48541 tggatctgct gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac
    48601 atttcccgtg ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt
    48661 aatctttctt cacctgcttt caaaggccag cctcgcgctg gcctttttct tttacctcac
    48721 cgcggtggat aggcattcac gccgcatccg gcaaaaagct gcccgcacaa aaacatcatt
    48781 cttcctcaac acgttttatc gcaaattatc atcattactg aaaccgatta c
//
LOCUS       DAZGJW010000018        47309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZGJW010000018 DAZGJW010000000
VERSION     DAZGJW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.018"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..631
                     /gene="comR"
                     /locus_tag="ACHKIK_001095"
     CDS             <1..631
                     /gene="comR"
                     /locus_tag="ACHKIK_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO9082309.1"
                     /translation="ATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLADL
                     VEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFAA
                     IANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGEI
                     PTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(693..1232)
                     /locus_tag="ACHKIK_001096"
     CDS             complement(693..1232)
                     /locus_tag="ACHKIK_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO9082310.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1459..2763)
                     /gene="ndh"
                     /locus_tag="ACHKIK_001097"
     CDS             complement(1459..2763)
                     /gene="ndh"
                     /locus_tag="ACHKIK_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO9082311.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3163..3705)
                     /gene="ycfP"
                     /locus_tag="ACHKIK_001098"
     CDS             complement(3163..3705)
                     /gene="ycfP"
                     /locus_tag="ACHKIK_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO9082312.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3728..4753)
                     /gene="nagZ"
                     /locus_tag="ACHKIK_001099"
     CDS             complement(3728..4753)
                     /gene="nagZ"
                     /locus_tag="ACHKIK_001099"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO9082313.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4764..5588)
                     /gene="thiK"
                     /locus_tag="ACHKIK_001100"
     CDS             complement(4764..5588)
                     /gene="thiK"
                     /locus_tag="ACHKIK_001100"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO9082314.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5569..6210)
                     /gene="lpoB"
                     /locus_tag="ACHKIK_001101"
     CDS             complement(5569..6210)
                     /gene="lpoB"
                     /locus_tag="ACHKIK_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO9082315.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6224..6601)
                     /locus_tag="ACHKIK_001102"
     CDS             complement(6224..6601)
                     /locus_tag="ACHKIK_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO9082316.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6604..6963)
                     /gene="hinT"
                     /locus_tag="ACHKIK_001103"
     CDS             complement(6604..6963)
                     /gene="hinT"
                     /locus_tag="ACHKIK_001103"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO9082317.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7297..9486
                     /gene="fhuE"
                     /locus_tag="ACHKIK_001104"
     CDS             7297..9486
                     /gene="fhuE"
                     /locus_tag="ACHKIK_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO9082318.1"
                     /translation="MLSTQFNRDNQYQASTKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQHIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLIGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HIKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9546..10979)
                     /gene="ptsG"
                     /locus_tag="ACHKIK_001105"
     CDS             complement(9546..10979)
                     /gene="ptsG"
                     /locus_tag="ACHKIK_001105"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO9082319.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11274..12071)
                     /locus_tag="ACHKIK_001106"
     CDS             complement(11274..12071)
                     /locus_tag="ACHKIK_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO9082320.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12082..13086)
                     /gene="holB"
                     /locus_tag="ACHKIK_001107"
     CDS             complement(12082..13086)
                     /gene="holB"
                     /locus_tag="ACHKIK_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO9082321.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13083..13724)
                     /gene="tmk"
                     /locus_tag="ACHKIK_001108"
     CDS             complement(13083..13724)
                     /gene="tmk"
                     /locus_tag="ACHKIK_001108"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO9082322.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13714..14736)
                     /gene="yceG"
                     /locus_tag="ACHKIK_001109"
     CDS             complement(13714..14736)
                     /gene="yceG"
                     /locus_tag="ACHKIK_001109"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO9082323.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14739..15548)
                     /gene="pabC"
                     /locus_tag="ACHKIK_001110"
     CDS             complement(14739..15548)
                     /gene="pabC"
                     /locus_tag="ACHKIK_001110"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO9082324.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNIVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15668..16909)
                     /gene="fabF"
                     /locus_tag="ACHKIK_001111"
     CDS             complement(15668..16909)
                     /gene="fabF"
                     /locus_tag="ACHKIK_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO9082325.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(16997..17233)
                     /gene="acpP"
                     /locus_tag="ACHKIK_001112"
     CDS             complement(16997..17233)
                     /gene="acpP"
                     /locus_tag="ACHKIK_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO9082326.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17444..18178)
                     /gene="fabG"
                     /locus_tag="ACHKIK_001113"
     CDS             complement(17444..18178)
                     /gene="fabG"
                     /locus_tag="ACHKIK_001113"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO9082327.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNEIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18191..19120)
                     /gene="fabD"
                     /locus_tag="ACHKIK_001114"
     CDS             complement(18191..19120)
                     /gene="fabD"
                     /locus_tag="ACHKIK_001114"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO9082328.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLIASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVINFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19136..20089)
                     /gene="fabH"
                     /locus_tag="ACHKIK_001115"
     CDS             complement(19136..20089)
                     /gene="fabH"
                     /locus_tag="ACHKIK_001115"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO9082329.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20157..21227)
                     /gene="plsX"
                     /locus_tag="ACHKIK_001116"
     CDS             complement(20157..21227)
                     /gene="plsX"
                     /locus_tag="ACHKIK_001116"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO9082330.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21308..21481)
                     /gene="rpmF"
                     /locus_tag="ACHKIK_001117"
     CDS             complement(21308..21481)
                     /gene="rpmF"
                     /locus_tag="ACHKIK_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO9082331.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21533..22054)
                     /gene="yceD"
                     /locus_tag="ACHKIK_001118"
     CDS             complement(21533..22054)
                     /gene="yceD"
                     /locus_tag="ACHKIK_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO9082332.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22253..22837
                     /gene="yceF"
                     /locus_tag="ACHKIK_001119"
     CDS             22253..22837
                     /gene="yceF"
                     /locus_tag="ACHKIK_001119"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO9082333.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(22949..23908)
                     /gene="rluC"
                     /locus_tag="ACHKIK_001120"
     CDS             complement(22949..23908)
                     /gene="rluC"
                     /locus_tag="ACHKIK_001120"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO9082334.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24481..27666
                     /gene="rne"
                     /locus_tag="ACHKIK_001121"
     CDS             24481..27666
                     /gene="rne"
                     /locus_tag="ACHKIK_001121"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO9082335.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27862..28815)
                     /gene="flgL"
                     /locus_tag="ACHKIK_001122"
     CDS             complement(27862..28815)
                     /gene="flgL"
                     /locus_tag="ACHKIK_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO9082336.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     RQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28827..30470)
                     /gene="flgK"
                     /locus_tag="ACHKIK_001123"
     CDS             complement(28827..30470)
                     /gene="flgK"
                     /locus_tag="ACHKIK_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO9082337.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNTGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30536..31477)
                     /gene="flgJ"
                     /locus_tag="ACHKIK_001124"
     CDS             complement(30536..31477)
                     /gene="flgJ"
                     /locus_tag="ACHKIK_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO9082338.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31477..32574)
                     /gene="flgI"
                     /locus_tag="ACHKIK_001125"
     CDS             complement(31477..32574)
                     /gene="flgI"
                     /locus_tag="ACHKIK_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO9082339.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32586..33284)
                     /gene="flgH"
                     /locus_tag="ACHKIK_001126"
     CDS             complement(32586..33284)
                     /gene="flgH"
                     /locus_tag="ACHKIK_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO9082340.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33337..34119)
                     /gene="flgG"
                     /locus_tag="ACHKIK_001127"
     CDS             complement(33337..34119)
                     /gene="flgG"
                     /locus_tag="ACHKIK_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO9082341.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34257..35012)
                     /gene="flgF"
                     /locus_tag="ACHKIK_001128"
     CDS             complement(34257..35012)
                     /gene="flgF"
                     /locus_tag="ACHKIK_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO9082342.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35032..36237)
                     /gene="flgE"
                     /locus_tag="ACHKIK_001129"
     CDS             complement(35032..36237)
                     /gene="flgE"
                     /locus_tag="ACHKIK_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO9082343.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36262..36957)
                     /gene="flgD"
                     /locus_tag="ACHKIK_001130"
     CDS             complement(36262..36957)
                     /gene="flgD"
                     /locus_tag="ACHKIK_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO9082344.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(36969..37373)
                     /gene="flgC"
                     /locus_tag="ACHKIK_001131"
     CDS             complement(36969..37373)
                     /gene="flgC"
                     /locus_tag="ACHKIK_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO9082345.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37377..37793)
                     /gene="flgB"
                     /locus_tag="ACHKIK_001132"
     CDS             complement(37377..37793)
                     /gene="flgB"
                     /locus_tag="ACHKIK_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO9082346.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            37948..38607
                     /gene="flgA"
                     /locus_tag="ACHKIK_001133"
     CDS             37948..38607
                     /gene="flgA"
                     /locus_tag="ACHKIK_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO9082347.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38683..38976
                     /gene="flgM"
                     /locus_tag="ACHKIK_001134"
     CDS             38683..38976
                     /gene="flgM"
                     /locus_tag="ACHKIK_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO9082348.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            38981..39397
                     /gene="flgN"
                     /locus_tag="ACHKIK_001135"
     CDS             38981..39397
                     /gene="flgN"
                     /locus_tag="ACHKIK_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO9082349.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39437..40972)
                     /gene="murJ"
                     /locus_tag="ACHKIK_001136"
     CDS             complement(39437..40972)
                     /gene="murJ"
                     /locus_tag="ACHKIK_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO9082350.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGTMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVM
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41082..42005)
                     /locus_tag="ACHKIK_001137"
     CDS             complement(41082..42005)
                     /locus_tag="ACHKIK_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO9082351.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTVLWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42007..42654)
                     /locus_tag="ACHKIK_001138"
     CDS             complement(42007..42654)
                     /locus_tag="ACHKIK_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO9082352.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42665..43249)
                     /gene="rimJ"
                     /locus_tag="ACHKIK_001139"
     CDS             complement(42665..43249)
                     /gene="rimJ"
                     /locus_tag="ACHKIK_001139"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO9082353.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43485..44693
                     /gene="mdtH"
                     /locus_tag="ACHKIK_001140"
     CDS             43485..44693
                     /gene="mdtH"
                     /locus_tag="ACHKIK_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO9082354.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44757..45404
                     /gene="grxB"
                     /locus_tag="ACHKIK_001141"
     CDS             44757..45404
                     /gene="grxB"
                     /locus_tag="ACHKIK_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO9082355.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPPRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNLADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45538..46098
                     /locus_tag="ACHKIK_001142"
     CDS             45538..46098
                     /locus_tag="ACHKIK_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO9082356.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46204..47250
                     /gene="pyrC"
                     /locus_tag="ACHKIK_001143"
     CDS             46204..47250
                     /gene="pyrC"
                     /locus_tag="ACHKIK_001143"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO9082357.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
BASE COUNT        11426 a        12828 c        11770 g        11285 t
ORIGIN      
        1 ggcaactgac tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag tgttcgacag
       61 ggatgccgcg cttgataagg ccatgaaatt gttctggcaa cacggttatg aagcgacttc
      121 tcttgcggac ctcgtcgaag cgaccggagc caaagcgccc acgctatacg cggaatttac
      181 caacaaagag gggttatttc gtgccgttct cgaccgctat atcgatcgtt ttgccgctaa
      241 gcatgaagca cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg ctgactattt
      301 tgctgccatc gccaactgct ttaccagcaa agacaccccg gcaggctgct tcatgatcaa
      361 caactgcacc accctctccc cagattcagg agatatcgcc aatacgttga aatcacgcca
      421 tgcgatgcaa gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc gcggggaaat
      481 cccgacccac tgtgacgtga cacatctggc agaattcctt aattgtatta ttcaggggat
      541 gtcgatcagc gcacgcgaag gtgcatcgct ggaaaaacta atgcagattg ccggaacgac
      601 tttgcgttta tggcccgaac tggtgaaata agtcgcaaaa aaagcccctc agcctgagcg
      661 aatgaggggc caaattgcag agtacagcct tgttatactt tgttattcaa aatcagttgc
      721 ccattactat ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat tttgccctgc
      781 tgatcgccaa tcttataggt cacatcataa ccgagcattt tttctgactt gtcatacacc
      841 gttttacaac gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga gccctggatc
      901 tggttaccgg catatccacc acccagcgcc cccacaacag tggcgacatc tttaccgcga
      961 ccaccgccaa actgatgccc tatcacgccg ccagcaacag cgccgagcac cgacccggta
     1021 atgcgatttt catcctgcac cggtcgacga tgggtcactg tgacgttgcg acactcctga
     1081 cgcggtgttt taaccgtttc cttgattggg gttgcagaaa caacctgagc gtattgcggg
     1141 ccacgttcaa acacgttcag actggccact gccgctacgc ccagcgcagc tgcgacacca
     1201 atcccgatac ccgccaacat tgatttattc acgggaacac ctccttcttt gcagttatgc
     1261 caatcatgca agcgaaggag aaaagcagct atcagacaga agatgaaaag cgcgggaatg
     1321 gctggaaata cttaatgatt cggaggagtc tgaaggaatg cctgatgcga cgcttaacgc
     1381 gtcttatcag gcctaccgct cgtaccattt gtccagggcg gatgaggcgt ttatgccaca
     1441 tccgccagtg tacgtcgatt aatgcaactt caaacgcgga cggataacgc ggttaatact
     1501 ccccaccagc atcattaatc cggttttaaa gtaaccatgc agcgcaatct gatgcattcg
     1561 gtatagcgag atatatacaa agcgcgcaat tcgtccttca atcatcattg agccgcgcgt
     1621 caggttaccc atcaggctac caacggtgga gaagttcgac agcgatacca gcgaaccatg
     1681 atctttatac tgataatttt tcagcggctt accattcatc tgcgccagaa tgttgttcat
     1741 tgcgcaagtc gccatctggt gtgcagcctg agcacgcggc ggaacaaagc ccccttccgg
     1801 acgcgggcat gacgcgcagt cgccaatagc gtaaatgtct ggatcgcggg tggtttgcag
     1861 cgtcggttcc accaccagct ggttgatacg gttagtttca agaccaccga tatctttcag
     1921 gaagtctggc gctttgatcc cggctgccca caccatcaga tcagcctcaa tatattcgcc
     1981 atctttagtg tgcaggccgc cttcatcagc actggtgacc atggtttgcg tcagcacgcg
     2041 aacgccaagt ttcgttagct cgttgtgggc cgcagcagag atacgtggcg gtaatgcagg
     2101 caaaatacgt tctcccgctt ctaccagcgt tacgttcagg gcttcgttgg tcaggccttt
     2161 gtaaccgtag ctgtgcagtt gcttgaccgc gttgtgcaat tcagcggaga gttctacacc
     2221 cgtcgcgccg ccgccgacaa tcgcaatgtt cactttgcca tttgcgccca ggttggcgga
     2281 gtatttcagg aagagattca gcatctcctg gtgaaagcga cgcgcctggt gcgggttatc
     2341 gaggaaaatg cagttctctt tgacacctgg cgtattgaaa tcgttagagg tgctacccag
     2401 cgccattacc agggtgtcat aggcgatttt acgttccgga accagcagtt cacctttctc
     2461 atcgcgcagt tctgcaatag tgattgtttt cgcttcacga tcaatatcaa tgacggaacc
     2521 cagctggaac tggaaaccat gattgcgcgc atgggccaga tagctcaacg catcgacgcc
     2581 ttcatcaagc gagccagtcg ccacttcgtg cagcagcggt ttccacagat ggctgtggtt
     2641 acgatcgacc agcgtaattt tggctttttt cttgcgtccc agcttatgcc ccagctgtgt
     2701 tgccatttcc agcccaccag caccgccgcc gacaatcaca atctttttca atggcgtagt
     2761 caacgtgacc cccttaaatt tattaaccaa ttgttaatta atggttatta acatagctct
     2821 taattaacaa caggttacag caatgaaaat gttcttcgct attgagaata acatgaatgg
     2881 tgcattggtc ataccaaaat tgatgttaat caagttttgt tgctgaaaag ataggcgact
     2941 ttaccgggtg agatcaaaaa acagagttta ttgtttatta tttgagcaag tccggaaggg
     3001 cgatataggg tgaagcgtat atcgagggcg tttggtcgtc ctgataagag gcgcatcagg
     3061 cattgatgct cgaatgtcgg atgcggcgtg aacgccttat ccgacccaca gaatccggag
     3121 cgcatcaggt accggcaagg ttacctgatg cgacgagggc atttacccga gggttttgaa
     3181 cgctttaatg cgctgtaaat gcggggagat attcttgaat ttgtgcgtct gctcttcgtc
     3241 ccagacaatc tcgtaataat gatgcagctc ttcagatgtc cgctggctgt taagcgcttc
     3301 atcattacgc gacaaaatca ccaggcaacg atcgcgattc ttctcacgga agttggtcac
     3361 acacttagtg gcaatatcgg catactcttc cgggcgatca atcttgcctt ccatgttctc
     3421 ataagggaac aaattagggt tgaagatcac ctggcggata tcgcaaagaa aaccaatccg
     3481 ttccgcccag tatccgccca gaccaacgcc gcaaatcagc ggacgctcgt cgacgttcag
     3541 ttgcaacatt ttgtccactt ctttgagcag atgctgcata tcatgtttcg gatgccgcgt
     3601 actgtagctt atcaagcgta catccgggtc aataaactgc aattgtaaga ctttctcgtg
     3661 gttacccgga ctgttagagt caaaaccgtg taaatagata atcatcgctt cctcacataa
     3721 gccagggtta gtgacctgct ttctcttcct gccagcgttc atgtaactga ttcagacggg
     3781 tgctgatcgc tttccagcga gccgagtcca tcagttcctg tcgcgaaaat gaacctttat
     3841 gatacaaacg tgtaacacgt tctgccttga tcggtgacag attatctaac acgctgacgg
     3901 cccctttacg attattgcag accaggatca tatcgcaacc cgcatccagt gatgcctgcc
     3961 cgcgttcggc ataactgccc ataatcgcgg caccttccat cgataaatcg tcagagaaaa
     4021 tcacgccgtc aaaacccagt tcctgacgca aaacggtttt cagccagtag ggagaaccgc
     4081 tcgccggacg cggatcaaca tcactgtaga tcacatgcgc aggcataatg gcgtcgagtt
     4141 tattttcgcg gattaaggaa ctgaagaccg acatatcttt agcgcgaatc tccgcttgtg
     4201 gacgtggatc gcacggtgtt tctttgtgtg agtctgccgt tactgcaccg tgtcctggga
     4261 agtgtttccc ggtcgttttc attccggctt catgcatacc atcaataaac cggctggcaa
     4321 ttgccagggc tttttgtgga tcggcatgat aagaacgctc gccaatcgcc gcgctgatat
     4381 gcccgacatc cagcactggc gcaaagctga tatcaatatc catagcgatc atttcgctgg
     4441 ccatcagcca acccgcctct tgcgccagtt tgccgccctc ttccattcct gacagcgcag
     4501 cgaatgattg cgccgctggc aagcgggtaa aaccttcacg aaaacgctgc acgcgtccac
     4561 cttcctgatc taccgccacc accagatgat tgcgcgaagc tgcgcggatc tggcgcacca
     4621 gttcacgcaa ctgggcagga tcatgatagt tacgcgtaaa gagaatcagc cctcccacca
     4681 gcggatgcgc aagtatttca cgctcttccg cgtccagttc gtaaccttcg acatccaaca
     4741 ttactggacc cacactgctc tccttattgt tttattaata gctgccgcca ggtgtcatcg
     4801 gccagcctga taaattgttg atcgccggtt tgtcgccagc ggtactcaaa ccaccctgct
     4861 ttgagcatca gcagccaggg aaaccatcgc ctgacctgac gccataattg cgccggataa
     4921 atcttcgcgc gagtggcata gtcattgacc aattgccggt gctgttcagt attttccacc
     4981 cacactgccg ccagttccag cgcgatatca ccatctccgg catactccca gtcgatgagt
     5041 tttaaccctg acgcgctatg cactaaattt ccggcgtgga catccatatg caatggactt
     5101 aagcgtaaag gccgtggttc ccgcgctttg cgcagacgtt ttaacattcg cagccaaccc
     5161 actgtccgcc gcgccggatc gctttgctgc cagtacagtt ccagtaacgg caacagcgtt
     5221 attcgccagc caaaacgtgg ttgttgatgt agataataca gcaagcctgc cagttcgttg
     5281 gtatccggca aatacgtttt tacctcgccg ggcagatagt cgactaccat ccagtcacgg
     5341 agatataaat gcggcttcgg tgcaatgctt gcgggtagtt gtgataaagc ccgatactgg
     5401 cgcaagaacg cggactgcgg cgcatcagga tcgtgcggct gacgcacaac aaaacgctgg
     5461 ccctgatgtt caatgagaaa actcccgcca ctaagcccac tattaaacgt cgtgacggga
     5521 tgaaactgcg ggaaaaagcg cgacagcaat tcgtcgcgca tgatgggatt attgctgcga
     5581 aacggcacct ttacctgacc agataatttc gcccgtctgc accagcatca gctgcatttg
     5641 tagggtcgga gcgttaacgt tgccagaggc gctggagtac agcacgtaat gagcgccgac
     5701 attgcgggca atgcctatgg ctttactacg ggtacctaaa ctgtcctgcg gcgacaaacc
     5761 taactgttgc ttagccatcg acagctgctg ggcggaaacc agggtaaatt tcccgttatt
     5821 agccagcgca tttcgcagcg tttcggtcgc ttctgcggca ttcagcgaac cgttagtacg
     5881 gttgttaacg ctatcaacca gcaggacgct acccgcagtc accccgtcag ccccaagcat
     5941 cttactgacc atcggctgca ttgcgccatt ccagtcataa tggcgaatat gcggcgcagg
     6001 cggtgccgtt tgatcttcgt gctcaattgg gcctggctgc tgcgggatcg tcggcaccga
     6061 gggcactgtg gggacaggct gttgtggctc ggctggttgt tccggcgctg gtttcacttc
     6121 ttctaccggt gcaggttcac gttgccccac acacccggcg agaaacatcg ccagcgcggt
     6181 aatcaaggcg tagcgactca tttttgtcat caagattcac cccttacaaa tatagataaa
     6241 gtctgacttt gtgcgccccc agaaaattgg cgctgccgta cagcgttacc gccgaatgtg
     6301 cgggaatggt aacgctgcgt ggcctttcca gaggatgcat ctccagccct ctggcgtcat
     6361 accagtaaaa acgataatga acggtaacgg gttcttgcct ttcgttataa agcgttgagg
     6421 atgctgaagg ttgaatatca gacgtcgaaa ggacgggctt ttctgcactg atgcccgcag
     6481 ccaataaaga tgactccatc accagcgatt gctcatcatt caccggaatt tccggatgtg
     6541 aacgacagcc caccagcagt aacaacgcca gagacaccag cccaaagcat ccttttctca
     6601 tcgttacaga cctttatgtg ccagcattgg tcccagcgga cggccaccca acaagtgcat
     6661 atggatgtgg taaacctctt gtccgccatg gcggttggta ttcatgatca gacgatagcc
     6721 atcttcggca ataccttctt gctcagcaat ttttgccgct acggtgatca tgcgtcccag
     6781 cgcctgctca tgctcagctg acacgtcgtt cacagtcgga atgaggatat tcggaatgat
     6841 cagaatatgc gttggcgctt gcggcgaaat atcgcgaaac gccgttacca gatcatcctg
     6901 gtagacgata tcggagggga tctcacgacg aataattttg ctgaatatag tttcttctgc
     6961 cacgacattt tcctttttca aatagccatg cgttgcgcca tgctacgccg cgccacactg
     7021 cgagtataga gtatggtcga gttaaacctg ctctttcaac cttaactcac gatttcttaa
     7081 gcaaaaaatc gttttgctgc cgtatatatc gccattattc ccatttctgc ctgcgaatgc
     7141 gctgaaaaga cattctctgt ttggtttaca cgtcaaatat ggatgcgtat atttctcatt
     7201 tgcatttaca tacaaaatta ttcgcacact aaatataacc gctcatcacc ctcctccggc
     7261 atgagcctga cgacaacata aaccaagaga tttcaaatgc tttcaacaca atttaacagg
     7321 gataatcaat atcaagccag caccaaacca tcactacttg ccggttgcat agcattggca
     7381 ctattacctt ctgccgcttt tgccgctcca gccactgaag aaacggtgat tgttgagggt
     7441 tcagccacag ctccagatga tggcgaaaat gattacagcg taacgtctac ctctgcgggt
     7501 accaaaatgc agatgactca acgtgatatt cctcagtcgg tcactattgt tagccagcag
     7561 cggatggaag atcagcagtt acaaacgctg ggcgaagtga tggaaaacac gctggggatc
     7621 agcaaaagtc aggcggattc cgatcgtgct ctttattatt cccgcggatt ccagatcgat
     7681 aactatatgg ttgatggtat ccccacctat tttgaatcgc gctggaatct gggcgacgca
     7741 ctttctgata tggcactctt tgaacgcgta gaagtagtgc gtggcgcgac aggactcatg
     7801 accgggacgg gtaatccatc tgcggcaatt aatatggttc gaaaacacgc gaccagtcgt
     7861 gaatttaaag gcgatgtctc ggcggaatac ggtagctgga acaaagaacg gtatgtggcg
     7921 gatttacaaa gcccactcac cgaagacggt aaaatccgcg cgcgaattgt cggcggctac
     7981 cagaataacg actcatggct ggaccgctac aacagtgaaa agaccttctt ctccggcatt
     8041 gtcgatgctg atttaggcga tcttacgacg ctttcagccg gttacgaata tcagcacatt
     8101 gatgttaata gcccaacctg gggcggttta ccgcgctgga atactgatgg cagcagcaac
     8161 agttacgatc gcgcacgcag taccgcacct gactgggcgt acaacgataa agagatcaac
     8221 aaggtcttta tgaccctgaa gcagcggttt gctgatacct ggcaagcgac gctgaatgcc
     8281 acccactctg aagtcgaatt tgacagcaaa atgatgtatg tcgatgccta tgtaaacaaa
     8341 gcggatggta tgctgattgg gccatacagt aattatggac ctggctttga ttatgtcggc
     8401 ggcaccggtt ggaacagtgg caaacgtaaa gttgatgcgc tggatttgtt cgctgacggt
     8461 agttatgaat tgtttggtcg tcagcacaat ctaatgtttg gtggcagtta cagcaaacaa
     8521 aacaatcgtt acttcagttc atgggccaac atcttcccgg atgaaattgg cagtttctac
     8581 aactttaatg gcaatttccc acaaaccgac tggtcaccac agagcctggc gcaggacgat
     8641 accacacata ttaaatcgtt atatgctgcc actcgtgtca cccttgccga tccgctgcat
     8701 ctgatcctcg gcgcacgtta taccaactgg cgggttgata cgctgactta cagcatggag
     8761 aaaaaccaca ccacgcctta cgctggtctg gtgtttgaca tcaatgacaa ctggtcgacc
     8821 tacgccagct atacctctat tttccagccg caaaatgatc gtgacagttc aggcaaatat
     8881 ctggctccaa tcaccggtaa caactacgag ctgggtctga aatcggactg gatgaatagc
     8941 cgtctgacca ccacgttagc catcttccgt attgagcagg ataatgtcgc tcagtccacc
     9001 ggtacaccta tccccggcag caacggcgaa accgcctata aagcagtgga tgggacagtc
     9061 agtaaagggg tggaatttga actcaacggc gcaattaccg acaactggca gctgacattt
     9121 ggcgcaacgc gctatattgc agaggataac gaaggaaacg ccgttaatcc taatctgcca
     9181 cgcaccacgg ttaaaatgtt caccagctat cggttgcctg tcatgccaga gttgacagtc
     9241 ggcggtggtg ttaactggca aaatcgcgtg tataccgaca ccgtgacacc gtatggcacc
     9301 ttccgcgccg agcaaggtag ctacgcgctg gtggatctct tcacccgcta ccaggtgacg
     9361 aaaaacttct cgttacaggg gaacgtcaat aacctgttcg acaaaaccta cgataccaac
     9421 gtggaaggtt ctatcgtcta cggcgcaccg cgtaatttca gcattaccgg cacgtatcaa
     9481 ttctgaatag cacctgtaaa aaaggcagcc atctggctgc cttagtctcc ccaacgtctt
     9541 acagattagt ggttacggat gtactcatcc atctcggttt tcaggttgtc ggatttagta
     9601 ccgaaaatcg cctgaacacc agaaccagca accactacgc ccgctgcgcc cagtttcttc
     9661 aggccagcct gatccacttt agacacatca gcaacgctga cgcgcagacg ggtaatacat
     9721 gcgtcgaggt tagtaatgtt ttctttacca ccaaatgcag caaccagagc cggtgccatt
     9781 tcgctggtac ctgtcgcttt tgcatcttca gtcgcgtctt cacgacccgg cgttttcaga
     9841 tccagtgctt taatcagcac gcggaagatg gtgtagtaaa caatcgcata accgataccg
     9901 acgatcggga acagccacag tttgctgctg ttaccagaca gaacgatgaa gtcgatcaga
     9961 ccgtgcgaga acgacgtacc gtcacgcatc cccagaagaa tacagattgg gaatgccagg
    10021 cctgccagaa tcgcgtggat gatgtacagg atcggcgcaa cgaacatgaa ggagaactcg
    10081 atcggctcgg tgataccggt caggaacgag gtcagcgccg cggagatcat aataccgccc
    10141 actttcgcgc ggttttctgg tttagcagag tgccagatag caattgcggc agctggcaga
    10201 ccgtacattt tgaacaggaa gccaccagac agtttacccg cagtcgggtc acccgccata
    10261 taacgcggaa tgtcgccgtg gaaaacctga cctgctgcgt tggtgtattc accaatctgc
    10321 atctggaaag gtacgttcca gatgtggtgc agaccaaacg gtaccaggca acgttcgatg
    10381 aaaccgtaaa tgccaaacgc aactaccggg ttctggtaag cagcccactg agagaaggtc
    10441 tggattgcag aaccaatcgg cggccaaatg aaggacagca caacgccagt aaagatggca
    10501 gccaggccag aaatgatcgg cacaaagcgt ttaccggcaa agaagccaag atactcaggc
    10561 agcttaatac ggtagaaacg gttaaacatg tacgctgcga tcgcaccgga gataatccct
    10621 ccgagtacgc cagtatccgc caggtgttta gaggcgattt cttcagcagg taaatgcagt
    10681 accagtggcg caaccacggc catggtttta accatgatgc cataggcaac aactgcggcc
    10741 agcgcggata cgccatcgtt attggtaaag ccgagggcga caccgatcgc aaaaatcagt
    10801 ggcatgtttg caaagacgga accgcctgct tctgccataa catgcgatac aacggcgggc
    10861 agccagctga aattcgcgga accgacgccc agcagaatac ctgcgatagg cagtacggat
    10921 accggcagca tcagcgattt accgaccttt tgcaggttag caaatgcatt cttaaacata
    10981 attgagagtg ctcctgagta tgggtgcttt ttttacgttc tcacgcgtgg caagggggga
    11041 gagcctcgcc gtgtacaggg catctaagcg ccctttattt attacacaga gtaaaataat
    11101 tcagtgccaa tttgtttgac ggctatcacg tttcaacttc agatcattgg agaagttgca
    11161 cattttgaca aaacatatgt cacaatattt caaacgtggt tattcaccgc ccaattttcg
    11221 gcggtgaact ttactcgttt tagccattaa ttacgagctt taaaaaaact cattcaacgg
    11281 atggattgaa ggcgggaagc gtcgatgtga aacagacggg cgaagttatc ggtggttacc
    11341 tgcgccagtt cttcaacggc aacacctttc aacacagcca tgtattctgc aacgtcacga
    11401 accatcgcag gttgattctc ttttcctcga tgcggtaccg gcgcaaggta aggtgagtca
    11461 gtttccacca gtaatcgatc cagggggaca taacgcgcag catcgcgcag ttgctccgca
    11521 ttacggaagg tcacaatgcc ggaaaaggag atgtaaaatc cgagatccag taatttaccc
    11581 gccgtttctc tgtcctctgt aaaacagtgt agtacgccac cgcaatccgt cactttttct
    11641 tcgcgcagaa ttgccagcgt atcggcacgg gcgtcacggg tatggacgat aaccggcttg
    11701 ttcagttcac gaccaatctg gatatgatgg atgaaggact cttgctgacg tactttagtt
    11761 tccggtgtgt aataataatc cagcccggtt tcacccagcg ctacaacacc ctcttctgcc
    11821 gccagacggc gtaaatcttc tacatcgtag ggatcattct ggttgagtgg atgcacgcca
    11881 caagaaaata cgacgttgtc acgttcgcct accagatccc gcatatgtaa ataacccggt
    11941 aatgttgtgg cgactgccag acaaaatttc acatcgcgtg cggcggcttt cgccagaacg
    12001 tcatccacgt ccttatgcaa agattcataa tccagaccat cgagatggca gtgtgagtcg
    12061 actaaaaaca tgatgtctct cttaaagatg aggaaccggt agcacaacgc ccggttgcag
    12121 gtaatgctca atacgcagta aaagatcggt gatgagaagc tcgcggttga tgcctgtaac
    12181 agacattaac tgttcacgaa tgtggcaaac atcccccagt atagcctgca ggcgcgaggg
    12241 agaaagatgg tttgccagtt cggcgaccag gcccggcaca tcaacattgg tcacctgcgc
    12301 agcaccatga tggcgtttta gcgcatccat cagcaacgtt gccagccagt gtaaacgcgc
    12361 cggagcttgt tcatgattaa gggccgctag cagcgaatac cagtcgcccg atggcacgct
    12421 atatgccaac gcctgacaca atgtttcacg agcctgccag ttatctccct gaaacaacgc
    12481 cagtgccgcg ccaggcgaac cggcgcttaa gcgcaatgcg gcaagtaatg catcctgtga
    12541 cattgtcact tcgcgtgaaa gccaggtcac ggcgtactgt tccggcggcg gcgcaaggta
    12601 atgtaaccga caacgactac gtaatgttgc cagtaaacgt tcaggctcgc gggtagccag
    12661 gaaaaaccag gtttctgctg gtggctcttc aagcgttttc agcaatgcgt tagccgcggc
    12721 gtcggtcagt aaggcagcat cggttaccca gacaactttc gcaccaccta ggcgtgcgtg
    12781 ctcattcagc ttttcggtga cctcacgtac cgcatcaatg cccagcatat ttttcccttt
    12841 ttcgggagcc agggtgtagt aatcgggatg cgtgccagcc tgcatcaact gacatccacg
    12901 acagtgaccg caacttttgt ggccctgcgg ttgttggcag agtaaataac ggctcagggc
    12961 gtagattaaa gcatcatcgc ccatgcccgg taacgcctga atgagtagcg catggtgacc
    13021 tcttccggcc tgatagctgg ctaccagttt ttcgaaatca ggtcgtaacc atggatacca
    13081 tctcatgcgt ccaactcctt aacccagtgg gtcacggtag tgcggattgc atccatcacg
    13141 gcctccagcg gctgggtggc atcaatggta tgaatacttt tatcttgtgc agccagttcc
    13201 agatagcggg cgcgggtgcg attaaagaaa tcgaaagact cttgctcaat acgatccagc
    13261 tcgccgcgcg cacgcgcgcg ttttaagcca acttccgggg taacatcgag atagagcgtt
    13321 aagtcggggc gaaaatcccc gagaacagca tcacgcagtg ttgccagcat atgttggtca
    13381 ataccacgtc ctccgccctg atacgcctga gtggagagat cgtggcgatc gccaatcacc
    13441 caggtgccgt tagccagagc tggtttgatg accgtttcta ccagttgaac gcgcgcggca
    13501 taaaacatca gaacttcggc tttatcggta atgacttcat cgcctaccga tttgatatcc
    13561 agcaccaggc ttcttaactt ttcggcaagt tgcgtaccgc caggttcccg agtgaaaacc
    13621 atgtcgcgga tacccagttg ctcaagcgtc tcaaccacca cattacgcgc ggtagttttg
    13681 cctgcgcctt ccagcccctc aatgacgata tacttactgc gcattttttt ccttaagcac
    13741 tttcagataa tcctgcacag acttgttatg gctggcaaga ttggtattaa acgtgtgacc
    13801 acctttacca tcggccacaa aatagagata cggcgttttt gccggatgcg cagcagcctt
    13861 cagcgaatcc gcccccggcg tagctatcgc acccggcggc aaaccggtaa tggtataggt
    13921 gttatacgct gtcggcgttt ccaggtctgc acgagaaagt ttgccattat aacgctctcc
    13981 catcccgtaa atcacggttg ggtcggtctg caggcgcata ccaatgcgta aacggttgat
    14041 aaataccgag gcaacctgat cgcgttcact ggcaacggcg gtttcttttt cgataattga
    14101 tgccatcgtc accagctgat ttttatcttt ataaggcaga ccgtccgcac gcccttccca
    14161 ggcgctatcg accgctttca ccattttctt gtgcgctcgc ttgagtaacg cgacatcggt
    14221 ggtattggcg gtatacatcc aggtgtctgg ccagaaccaa ccttcaatcc actccgggtt
    14281 ttccagttca agtgcctgcg ctacggtggc gtacttatcg tcactcagcg tgtgcttgat
    14341 atacggagcc tcacgcaatt gcttgaggta gtcgctcaga cgcatccctt ctaccagtcg
    14401 cagcgggaac tgtgcttctt taccgctttc caacaatttc agcatctcgc gcacggtcat
    14461 ctgcggtgta aagcggtaag tcccggcttt aaagtgagaa agatccggtt cgatacgcag
    14521 cagccattga aaaacccgtg ggcgattgat gatcttatca gcataaagct gttcaccgag
    14581 cgccagacgt ccggtccctg gcttcagggt aaatatcgtc tcttctttga taagcaattt
    14641 gctgtcggca agatggcgaa ccttccagac gcccacacca gcggcgatac ccagtaccac
    14701 cagcaataac aagataatca ataacacttt tttcatgact aattcgggcg ctcacaaagt
    14761 ggggctaaat attcatataa cgttgctgac gaaaaggaga catcgccaca ggcacgtacg
    14821 ggcatcactg gcattaacgc attacaaata accatctcat ctgcctgcaa cgcctcttcc
    14881 agagaggctt gcacttcgac aagctgataa gaggattgtg ccagcaaacg gatacagaat
    14941 tgtcgcataa tgccgttaac acctgcctga tccagtcgcg gcgtataaac tatattgccc
    15001 ttccgccaga acaaattagc cgcacagcat tccgtaaccc acccttcgct gtcaaggacc
    15061 agcgcctcat cagcgtttgt ctgctcaaga tgagagcgga tcaatacttg ctcaagccga
    15121 ttaagatgtt taatacctgc aagatgagga ttgcgcccca gccgcaccgg gcttagcgcc
    15181 aacgtcatcc cttcgttacg caaacggtcg taatgtgcag gataaaccgt aacggagaga
    15241 atccgcgttg ccggtccact gttcaatgtg ctgtaccctc gcccgccact accgcgactg
    15301 atcacgactt tcagcacacc attttgctgt tctgctgcca gcgttttcat ctcttgttca
    15361 agctgaggcc agaagtcaca gaaaatcatc aaccgctgac aagcatcctg tagtcgctgg
    15421 atatgcgccg ataacaaact gactttaccg tcgataactc tggcggtggt aaaacaacca
    15481 tcaccaaact gcgttgcccg atcgcttact gccagcgatt cctgcttata accgttaatt
    15541 aagaacatac cggctcctta tcagagaatt gttagtgtgg cagcatgttc actacggaac
    15601 aagtcggaat aaaagctaag aaaaaaggcc cgcaagcgga ccttttatat gggtggaaaa
    15661 tgacaactta gatcttttta aagatcaaag aaccattagt gccaccgaag ccgaaggagt
    15721 tacacagcgt gtattccatt ccgctgacct gacgtgcttc atgcggtacg aaatccagat
    15781 cgcaaccttc atccgggtta tccaggttga tggttggcgg aacagcctga tcgcgcagcg
    15841 ccaggatgga gtagatagat tctactgcac ccgccgcacc taacaggtga ccggtcatag
    15901 atttcgtgga gcttaccaac acacggcttg cagcttcacc gaagatggtt ttcaccgcct
    15961 gcgcttcagc tttatcgcca gccggcgtag aagtaccgtg cgcgttaacg tagccaatct
    16021 gactcgcttc aatgcctgca tcacgcagag catttgccat tgccagagct gcgcctgcgc
    16081 cattttctgg cggtgacgtc atatgataag catcgctgct cataccaaag ccgacgagtt
    16141 cagcgtaaat tttcgcaccg cgttttttcg cgtgttcgta ctcttcaagt accagcatac
    16201 cggcaccatc gcccagtacg aagccatcac gctctttatc ccacgggcgg ctcgccgctt
    16261 gcgggttatc attgcgggta gataatgcac gtgccgcgcc aaaaccacca acgcccagcg
    16321 gcgtactggc tttctctgcg ccacctgcaa ccatcacgtc agcatcgcca tacgcgataa
    16381 tacgcgcagc atgaccaatg ttgtgcacgc cggaagtaca ggcggtcgcg atagagatgc
    16441 ttgggccacg caggccatac atgatagtca gatgacctgc caccatgttc acaatcgttg
    16501 acggaacgaa gaatgggctg atcttacgtg gaccaccgtt catcagagat gtgtggtttt
    16561 cttcgatcag tccgaggccg ccaatcccgg agccaattgc ggcaccaatg cgggttgcgt
    16621 tctcttccgt tatttcaagg ccagaatcct gcatggcctg aacgccagcg acaattccat
    16681 attgaatgaa ggcatccatc ttgcgctgtt ctttgcgcga gataatgtcc tcacagttaa
    16741 aatcctttac taagccagca aatttcgttg cataggcgct agtatcgaaa tggtcgatta
    16801 ggctgatgcc actctgaccg gcaagcagag ctttccaggt agactctacg gtattgccga
    16861 caggagacaa catgcccagt ccggtcacaa ctacacgacg cttagacacg tttgtcctcc
    16921 agggagggaa aaaatgattc tagtgggaca aaaagataaa actcaggcgg tcgaacgacc
    16981 gcctggagat gttcacttac gcctggtggc cgttgatgta atcaatggca gcctgaacgg
    17041 tggtgatttt ctcagcttct tcgtccggaa tctcagtatc aaactcttct tccagagcca
    17101 ttaccagctc aacggtgtca agagaatccg cgcccaggtc ttcaacgaaa gaagcattgt
    17161 tggtaacttc ttcctgctta acgcccagct gttcgccgat aattttctta acgcgttctt
    17221 cgatagtgct catactctta aatttcctat caaaactcgc tttcgcgatg gttttcgtag
    17281 tgtataaaat gttgaaaaat ttgcaactaa atcccggcag gtcttaccac gattttacgt
    17341 tattttgagg ccttaccccc caataacgca aatcattttg cactaattat tactcataac
    17401 cacgcagact gcgcgcaaca tgagcatttt gtgcaaatcg cggtcagacc atgtacatcc
    17461 cgccgttcac atgcaaagtt tcacccgtga tgtaagctgc ttcgtcggat gccaggaatg
    17521 caaccgcgtt ggcgatttcc tgtgcgccgc cgaggcgacc cgcaggaacc tgcgccagga
    17581 tacccgcacg ctggtcatcg ctcagcgcac gtgtcatgtc cgtttcaata aagcccggag
    17641 caacaacgtt tacagtaata ccgcgtgacg caacttcgcg cgccagtgat ttactgaagc
    17701 cgatcaagcc cgctttcgcc gcagcgtagt tggcctgacc gccatttccc atggtaccaa
    17761 ccacagaacc gatagtgata atacgaccat gacgcttttt catcatagcg cgcattaccg
    17821 cttttgacag acggaaaaca gatgaaaggt tggtttcgat aatttcgttc cactcttcat
    17881 ctttcattcg cattaacagg ttatcacgag tgataccggc attattgacc aggatatcca
    17941 cttcaccaaa ttctgcgcga attttttcca gaacagattc gatagatgcc gggtcggtca
    18001 cattcaacat cagacctttg ccgttggcac ctaaataatc gctgatcgcc tgagcgccat
    18061 tttcactggt cgcagtgcca ataactttcg cgccacgggc tgcgagcgtt tcagcaattg
    18121 cgcggccaat tccgcggctt gcaccggtta ccagtgcgat ttttccttca aaattcatga
    18181 ttttcctctt ttaaagctcg agcgccgctg ccatcgctga aggttcgttc agcgccgagg
    18241 cggtcagggt gtcgacaatg cgtttcgtca ggccagtaag caccttgccc gggccgactt
    18301 catagagatg ttctacgcct tgcgctgcca tgtactcaac agacttcgtc cactgaaccg
    18361 ggttatacaa ctgacgtacc agtgcgtcac ggatggcatc accattggtt tcgcatttca
    18421 catcaacgtt attcacaaca gaaactgttg gtgcgttaaa ggtgattttc gctaattcta
    18481 ctgccagttt gtcggctgct ggtttcatca gcgcacagtg agacggtacg ctcactggta
    18541 acggcagcgc gcgttttgcg ccagcagctt tacaggcagc gccagcacgc tcaaccgctt
    18601 ctttatgacc ggcaataacc acctgtcccg gagagttaaa gtttaccgga gaaacgacct
    18661 gaccttctgc agcttcttca cacgctttcg caatagacgc atcatccaga ccgatgattg
    18721 ccgccatagc gccagtgcct tccggtacgg cttcttgcat gaacttaccg cgcatctcaa
    18781 ccagacgcac cgcatcagcg aaattaatca cgccagcgca aaccagggcg gagtattccc
    18841 ccaggctgtg accggccatc attgccggtg ctttaccgcc ctgctgctgc catacgcgat
    18901 acagcgcaac agatgcaatc aacagcgctg gctgggtttg ccaggtttta ttcagttctt
    18961 cagctggccc ctgctgggtc agcgcccaca ggtcgtagcc cagcgccgca gaagcttcag
    19021 caaacgtttc ttcgacaatt ggatagctcg ccgccatatc agccagcatt ccaacggttt
    19081 gagaaccctg tccagggaac acaaatgcaa attgcgtcat gttttaatcc ttatcctaga
    19141 aacgaaccag cgcggagccc caggtgaatc caccgccaaa ggcttcaagc agaaccaact
    19201 gccccggctt aatgcgcccg tcgcgtacag cttcatccag cgcgcacggg acagaggccg
    19261 cagaggtatt accgtggcga tccagcgtca ccacgacatt gtccatagac ataccgagtt
    19321 ttttcgccgt tgcactgata atacgcaggt tagcctgatg cggaaccagc cagtccagtt
    19381 gagaacggtc aagattattt gccgccagcg tctcatcaac gatgtgcgcc agttccgtta
    19441 ccgcaacctt gaagacttcg ttgcccgcca tcgtcagatg aattgaattc tctggattca
    19501 cacggtcagc attaggcagc gtcagcaact caccatagct accgtcggca tgcagatggg
    19561 tggagatgat tcccggctct tcagaggcag ccagcaccgc agcgcccgcg ccatcgccaa
    19621 aaataataat agtcccacga tcggttggat cgcaggtgcg cgccagtaca tcggaaccga
    19681 cgaccagagc atacttcacc gccccagatt tcacgtattg atcggctacg cttaatgcat
    19741 aggtgaaacc tgcgcaggct gctgcaacgt caaatgccgg gcaaccttta atgcccaaca
    19801 tgctttgaat ctgacaagct gcgctcggga aagcgtgcgt agcagaagtc gttgccacaa
    19861 cgatcaggcc aatctggtct ttctcaatgc ccgccatctc aattgcgcgt gtcgccgctt
    19921 caaagcccat ggttgaaacg gtttcgtttg gcgcggcaat gtggcgttcg cggataccgg
    19981 tacgagtgac aatccactcg tcagaggtgt ccaccatttt ttccaaatcg gcgtttgtcc
    20041 gcacttgttc gggcagatag ctgccagtac caataatctt cgtatacatg tacgctcagt
    20101 cacttttcgg ttatataccg tcacttgcaa actgcgagtt cgctggcagc gtcctgctac
    20161 cgcagagttc cgcttttgcc accgtccagc agctcaaaac cagctgggta tacagattcc
    20221 aggcgagcgg caattcgctg aggaacttgt cgctgcaccg cctgcactgc ctgttcaatc
    20281 gcgaccgcaa aagctcgctg attggctgca ccatgacttt ttatcaccgt gccgcgcaat
    20341 cctaacagac aggcgccgtt atactggtcg gggttgaggt gactgaatcg cctcgtcagg
    20401 ctcttttgta gccaacgctt taataacagt agccaccacg accgtttttt cccttcaccc
    20461 tgagatttca gcagagaaag gaacatcctg acaacacctt ccatcgtctt taacgtgaca
    20521 tttcctgtaa agccgtcaca aaccagcaca tctgtcttgc cagttaacaa ctcattggct
    20581 tcaagatagc cgatataatt gatagaaggg attgttttaa gcaccgctga ggcatcccga
    20641 atactgtcga gaccctttac ttcttcttca ccaatattga gcaacgccac gcgaggattg
    20701 ggaatttcca ccacctcttc agccagaact gagcccataa tggcaaattg caccaacatt
    20761 gtgctgtcac aatcgacgtt ggcccctaaa tcgaggacca ccgttttgcc cttttgctga
    20821 tgtggtaata ccgtcaccag cgccggacgc tcaatcccct ccaggggctt gagtaataat
    20881 tttgccagcc ccatcagtgc cccggtatta ccggcactga cacaggcttg cgctcgacct
    20941 tctttcacca gctccagggc catacgcatt gaactcccac gactggcgcg gatagcttgc
    21001 gaaggccggg catcactggc gataactgac tgcgcaggaa taatctgcag acgcgaacgt
    21061 tgttcaaagt cagctttagc aagtaatggc gtgatggcgt cgggattgcc gactaaaaga
    21121 agagtgagtt gcgaattaga attcagtgcc tgcaatgctg caggcactgt cacggaaggg
    21181 ccaaaatccc ctcccatgac atctaacgcc agggttagac gtgtcaaggt atcgtcgccg
    21241 cccggtttgg tctttcccca gttgcctggg gaaaatcctc actaagcttc atcacgcaga
    21301 cgcgtgatta cttagcgatg accttgcggc cgcggtagta accgtcggca gtgatgtggt
    21361 gacgcaggtg tttttcacca gaagttttgt ctacagacag gctggtgact gcggtcagcg
    21421 cgtcatggga acgacgcatg ccacgtttgg aacgggttgg tttattctgt tgtacggcca
    21481 tggaccttac tcctcaatta cggtttatcc ccgatccaac ggggagcacc aattacttac
    21541 gctttaagct ggctaatacg gcaaatgggt ttggcttttg cgcttcttca ggcagttcac
    21601 caaagaccat gtccgcttcg gacacttcac agtgttcaga atcatgcacc ggaactaccg
    21661 gcaaggcgag gatgatttca tcttcaacca ttgcaagcag atcgatttca ccgaattcgt
    21721 taacctcaat cggttcatac gcttccggca gtgcttcagc ctgttcgtct gaacgcacag
    21781 gactaaaaca atacgttgtg tagacctgat gagtaaacgg cttcccgcaa cgctgacact
    21841 cgagcgttac cgtcaccttc gcatcgccgt ttaacactgc gagacgttgg ttatcgatag
    21901 cgaacgacat ggagcattcc acatcactgt ccacactgac tacggattcg gcgacgcgct
    21961 caacctgatc aggggtatag ataccctggt aatcaaggcg tttttgagcc gtacgaaccg
    22021 gatcgagagt caggggtaat tttacctttt gcatagggcg cgcatattaa ctttgtaacg
    22081 tcatagagtc aaagaaaaag gcagcctgtg gttgcctttt gccaataatt cgcacacatt
    22141 gcgggttact gctttatttt cgttcagtgc tgataacgaa catcaacctg caaaacgaag
    22201 cgcatagttt aaaatggcta tcatcaatac gctatatctg atggaaaaaa atatgcctaa
    22261 acttatttta gcctccacct cgccatggcg tcgcgctctt ctggaaaaac tgcaaatctc
    22321 ttttgaatgt gcagcaccag aggtcgacga aaccccacgc agcgacgaat caccgcgaca
    22381 gttggtgctt cgactggcac aagaaaaagc gcaatctctg gcgtcacgtt atccggatca
    22441 tttaattatt ggttcagacc aggtatgtgt tcttgatggc gaaatcaccg gtaaaccgtt
    22501 aacggaagaa aatgcccgtc tgcaattacg caaagccagc ggcaatatcg tcaccttcta
    22561 tactggactg gcgttgttta attcggcgaa tgggcatcta caaacagaag tggagccttt
    22621 tgacgtccat ttccgtcacc tgagcgaggc ggagattgat aattacgtgc gtaaggagca
    22681 tcccctgcac tgcgcgggta gctttaagag tgaaggattt ggcattacgc tgtttgagcg
    22741 tttagaggga cgtgacccta acacgctggt tggtttaccg ctaatcgcgc tgtgtcagat
    22801 gttacgtcgg gaagggaaaa acccgctgat gggatagttg tttgcgccgg atgcggttac
    22861 gtcgcatccg gcaatcggtg caggcggcct gatgcggcgt aaacgcctta tcaggccaac
    22921 cgcccattat caggcttata tgcttgtatt agcgcgcgtt acgcagcttt tgcaaacaac
    22981 gcttcaaacc ttcatccatc ggcgcttcga tacgcatcac ctcaccggtc cccggatggg
    23041 taaacttcaa cgctgcggcg tgcaggaaca gacgatttaa tcccgtgcct gcttcagtga
    23101 gctgtctgtc aaattcacgg tcaccgtagc gatcgtcaaa ggcaatcgga tgacccgcat
    23161 actgtgtatg cacacggatc tgatgagtac gtccggttac cgggctacaa cgcaccaggg
    23221 tggcaaatgc atagcgttct tccactttaa agcgtgtttc cgacggtttg ccttcctgac
    23281 tgacacgcac gatacgttcg ccgctttgca gaatattttt caataacggc gcttgaacgc
    23341 tcttcacatg cgactgccac tgaccgcgca ccagcgccag gtaatctttt tgcattcctt
    23401 tttcacgtaa ttgctcatgc agagaacgca acgccgagcg ttttttcgct accagcaaca
    23461 cacctgaagt gtcccggtcg agacgatgaa ccagttcaag gaaccgcgct tccgggcgca
    23521 acgcccgcaa accttcgata acgccgaagc ttaaaccact gccgccatgt accgccgtac
    23581 cggaaggttt attcagcacc aggatatgat catcttcata taagatgacg tccgccagcg
    23641 ccgccacctt ttgcagatgt ggcgaaaccg cctcttcttc ccgctcagca acgcgaaccg
    23701 gtgggatacg cacctcatca cccgcttcga gtttatattc aggcttaata cgttttttgt
    23761 tcacccgcac ttcaccttta cgcaaaatac ggtaaatcat actttttggt acgcctttca
    23821 attgggtacg caaaaagtta tcgatacgtt gccccgcttc gtcagcggtg atagcaacaa
    23881 tttttacgga tggagtctct gttttcatgg tgggcgattc taattagcca acaggattcg
    23941 cgccactcat ttttctatgc ttatatttag ttttacacct tattacttca ctacgtgatc
    24001 actttattga tggttattaa accaatcacc agcaagaagt gaaaaaactg tgagtaagcg
    24061 ggtgataaat ggtaaaagtc atcttgctat aacaaggctt gcagtggaat aatgaggccg
    24121 tttccgtgtc catccttgtt aaaacaagaa attttacgga ataacccatt ttgcccgacc
    24181 gatcatccac gcagcaatgg cgtaagacgt attgatcttt caggcagtta gcgggctgcg
    24241 ggttgcagtc cttaccggta gatggaaata tttctggaga gtaataccca gtctgtttct
    24301 ttgataattg cgctgttttt ccgcatgaaa aacgggcaac cgacactctg cgcctctttg
    24361 agctgacgat aaccgtgagg ttggcgacgc gactagacac gaggccatcg gttcacaccc
    24421 ggaaaggcgt tactttgccc gcagcttagt cgtcaatgta agaataatga gtaagttacg
    24481 atgaaaagaa tgttaatcaa cgcaactcag caggaagagt tgcgcgttgc ccttgtagat
    24541 gggcagcgtc tgtatgacct ggatatcgaa agtccagggc acgagcagaa aaaggcaaac
    24601 atctacaaag gtaaaatcac ccgcattgaa ccgagtctgg aagctgcttt tgttgattac
    24661 ggcgctgaac gtcacggttt cctcccacta aaagaaattg cccgcgaata tttccctgct
    24721 aactacagtg ctcatggtcg tcccaacatt aaagatgtgt tgcgtgaagg tcaggaagtc
    24781 attgttcaga tcgataaaga agagcgcggc aacaaaggcg cggcattaac cacctttatc
    24841 agtctggcgg gtagctatct ggttctgatg ccgaacaacc cgcgcgcggg tggcatttct
    24901 cgccgtatcg aaggcgacga ccgtaccgaa ttaaaagaag cactggcgag ccttgaactg
    24961 ccggaaggca tggggcttat cgtgcgcacc gctggcgtcg gcaaatctgc tgaggcgctg
    25021 caatgggatt taagcttccg tctgaaacac tgggaagcca tcaaaaaagc cgctgaaagc
    25081 cgcccggctc cgttcctgat tcatcaggag agcaacgtaa tcgttcgcgc attccgcgat
    25141 tacttacgtc aggacatcgg cgaaatcctt atcgataacc cgaaagtgct cgaactggca
    25201 cgtcagcata tcgctgcatt aggtcgcccg gatttcagca gcaaaatcaa actgtacacc
    25261 ggcgagatcc cgctgttcag ccactaccag atcgagtcgc agatcgaatc cgccttccag
    25321 cgtgaagtcc gtctgccgtc cggtggttcc attgttatcg acagcaccga agcgttaacc
    25381 gccatcgaca tcaactccgc acgcgcgacc cgcggcggcg atatcgaaga aactgcgttt
    25441 aacaccaacc tcgaagctgc cgatgagatt gctcgtcagc tgcgcctgcg tgacctcggc
    25501 ggcctgattg ttatcgactt catcgacatg acgcctgtac gccaccagcg tgcggtagaa
    25561 aaccgtctgc gtgaagcggt gcgtcaggac cgtgcgcgta ttcaaatcag ccatatttct
    25621 cgctttggcc tgctggaaat gtcccgtcag cgcctgagcc catcactggg tgaatccagt
    25681 catcacgttt gtccgcgttg ttctggtact ggcaccgtgc gtgacaacga atcgctgtcg
    25741 ctctctattc tgcgtctgat cgaagaagaa gcgctgaaag agaacaccca ggaagttcac
    25801 gccattgttc ctgtgccaat cgcttcttac ctgctgaatg aaaaacgttc tgcggtaaat
    25861 gccattgaaa ctcgtcagga cggtgtgcgc tgtgtaattg tgccaaacga tcagatggaa
    25921 accccgcact accacgtgct gcgcgtgcgt aaaggggaag aaaccccaac cttaagctac
    25981 atgctgccga agctgcatga agaagcgatg gcgctgccgt ctgaagaaga gttcgctgaa
    26041 cgtaagcgtc cggaacaacc tgcgctggca acctttgcca tgccggatgt gccgccagcg
    26101 ccaacgccag ctgaacctgc cgcgcctgtt gtagccccag caccgaaagc tgcaccggca
    26161 acaccagcaa ctcctgcaca acctgggctg ttgagccgct tcttcggcgc actgaaagcg
    26221 ctgttcagcg gtagtgaaga aaccaaaccg accgagcaac cagcaccgaa agcagaagcg
    26281 aaaccggaac gtcaacagga tcgtcgcaag cctcgtcaga acaaccgccg tgaccgtaat
    26341 gagcgccgcg acacccgtag tgaacgtact gaaggcagcg ataatcgcga agaaaaccgt
    26401 cgtaatcgtc gccaggcaca gcagcagact gccgagacgc gtgagagccg tcagcaagct
    26461 gaggtaacgg aaaaagcacg taccaccgac gagcagcaag cgccgcgtcg tgaacgtagc
    26521 cgccgccgta acgatgataa acgtcaggcg caacaagaag cgaaggcgct gaatgttgaa
    26581 gagcaatctg ttcaggaaac cgaacaggaa gaacgtgtac gtccggttca gccgcgtcgt
    26641 aaacagcgtc agctcaatca gaaagtgcgt tacgagcaaa gcgtagccga agaagcggta
    26701 gtcgcaccgg tggttgaaga aactgccgct gccgaaccaa ttgttcagga agctccagct
    26761 ccacgcacag aactggtgaa agtcccgctg ccagtcgtag cgcaaactgc accagaacag
    26821 caagaagaga acaatgctga taaccgtgac aacggtggca tgccgcgtcg ttctcgccgc
    26881 tcgcctcgtc acctgcgcgt gagtggtcag cgtcgtcgtc gctatcgtga cgagcgttat
    26941 ccaacccagt cgccaatgcc gttgaccgta gcgtgcgcgt ctccggaact ggcctctggc
    27001 aaagtctgga tccgctatcc aattgtacgt ccgcaagatg tacaagttga agagcagcgc
    27061 gaacaggaag aagtacaagt gcagccgatg gtgactgagg tccctgtcgc cgccgctgtc
    27121 gaacctgttg ttagcgcgcc agttgttgaa gaaatggctg aagtcgtaga agcccccgta
    27181 ccggttgccg aaccgcaacc ggaagtggtt gaaacgacgc atcctgaagt gattgccgcc
    27241 gcggtaactg aacagccgca ggtgattacc gagtctgatg ttgccgtagc ccaggaagtc
    27301 gcagaacacg cagaaccggt ggttgaaccg caggaagaga cggcagatat tgaagaagtt
    27361 gccgaaactg ctgaggttgt ggttgctgaa cctgaagttg ttgctcaacc tgccgcgcca
    27421 gtcgtcgctg aagtcgcagc agaagttgaa acggtagccg cggttgaacc agagatcacc
    27481 gttgagcata accacgctac cgcgccaatg acgcgcgctc cggcaccgga atatgttccg
    27541 gaggcaccgc gtcacagtga ctggcagcgc cctacttttg ccttcgaagg taaaggtgcc
    27601 gcaggtggtc atacggcaac gcatcatgcc tctgccgctc ctgcgcgtcc gcaacctgtt
    27661 gagtaataat tagctcaaag taatcaagcc ctggtaactg ccagggcttt tttatttcat
    27721 ctttgaatca acatattaaa attcatcaaa tggcatcctt gctaaccaac aatgcaaaat
    27781 aggcaaaaaa atgcccgccc cggcgatccc agagcaggca gacaaaacat acccagtttc
    27841 atgatatgtt ttaaagcgag cttatttgtt gagctggaag agcgacaatc cctgcatatc
    27901 ggtaaatgct ttatacgatg cctgcaatgc cgtttgctgc atgatgtaag atgaaatagt
    27961 tgcattccag tccacatcaa ccagatcgct catctgctgc gtttgcccta aagcgcgatc
    28021 gctacctaat gaatccagcg actccagttc gttcagctgc gtgcctaatt ccgcgcgaac
    28081 tgtaaggaca ttattcagcg agtttttcag tccacggttg gttttatcca atgccgcagc
    28141 ggcgatttct ttatccgctt cgctatccgc gaccggcgtt ttcagggctg cgatggcgct
    28201 atccagcatg gcaaaaagat tggtttcaga agcgctaccg tctggttccg ctaccgcgtt
    28261 actggtaata ctgtcgaaaa ttttgtcgcc cgtatgtcct atcaccatcg aacgcgaagc
    28321 atcgacctgt tgcctaatac tttctgctcc accgacgtat ttccctttct cttcgctaaa
    28381 cggcgcagtc tctgttttat aaccggcaaa aatgtagcgc ccgttaccgt cagtggtgtt
    28441 cgccagattc agcaactggt cacgaagccc ctggatatcc gtagccagcg aggcccggtc
    28501 atcgtcactc aaagtgccat tgctggcgta gacaattttt tcctgagcat tctggatagc
    28561 agtggtgacc tggctaagta cactctcttc cagtgacact ttttgagtgg cgaaagtacg
    28621 cgccagcgtg tactggctgt tttgcgcctg tgcctgggag agaacaaccg cttgtgatgc
    28681 tgcaatggga tcgtcagaag ggttaacgac tcgcttacct gtcgacatct gctcgccgta
    28741 cttcatccat tccgcctgag aattggtgat gccacgcatg ttttgctggt acatcatctg
    28801 ggtactgaaa cgcatcttat ctccccttag cgaatgttaa tcagcgcatc aaaaatcgcg
    28861 tttgccgtct gcagaacctg cgcattcgcc aggtaatact gctgaaaacg ttgcagattt
    28921 ccgtactcct catcgagatt gacaccggaa atcgactgct gctgattgga aagctgcgtc
    28981 accacattac cttgcgtggc gctactggtt ttcaacgtcg cggttttatt accgatatca
    29041 ctcactaacg aggcataagc gtcgttaaag gatttcgcac cgcccaccgt tttactgttg
    29101 ctttgcagat ccagcagggc ctgaccgttg cggttatcgc tatcacccgc atcttcttcg
    29161 ctcgccatcg ctattttcgc ttcgtcggtg attaatacat ccatgttgac gatagcgtca
    29221 cttactggtt tcagcgtgaa gctgtcgtta acagcaggcg ttcctgtaaa cgtcaactcc
    29281 agaccatcaa atgccacttt accgttggca tccggcgtca ccgtaaaagt ggtattactg
    29341 gcaaggcggg tgacctgcca ctgattatta tcgaacgaga ttttgtaatc agtcgccagt
    29401 accgcggagg catcagttac cgtggcaccg atcgcaacgt cacctgtgtt tttcgtgttt
    29461 tgcagaaccg cgggcttacc gatagcaaag aaatcttcac cagcatcgcc gttggcatca
    29521 aatccggctt tgtgttggct gttgaaagcc tcggcaaatg ccagcgccag ttgtccaagc
    29581 gtattacgcg tctggtccag atcctgagaa cggaatgtca gaatgccgcc cagcgacccg
    29641 gtattcagta atttctccgg gatctcaata ttgcctgccg tcccatcaac gtaagcgaca
    29701 gtcgtacgag aagggtcggc gctggaagga actgccgcca gttgccgcgc cgtacttccc
    29761 tgaaccagtg agtaaccatt ggccatcgtg atgttataag taccgccatc ctgaacgctg
    29821 acttctacgc caacaatctg gtttaattcg ctcaccagtt gatcgcgttg atccagcaga
    29881 ttgttaggtg acgcccctgc ccccacgcct gtcaggcgcg agatttgatc gttcaggctg
    29941 gcaatttgtt tagcgtaatt gttgatctga tcaacgctgg cacctatcgc gatattgacc
    30001 tgtttgtcct ggtcgcgcag atattgatcg gtggttttaa actgattcac caacccttct
    30061 gatttcccaa tcagcgcctg gcgcgctgcc gggtcttccg cgttactcac cagcgtttgc
    30121 aggctggtga agaaatcctg catctgtgtt gccagcgaag aggtactggt ggagagcata
    30181 ttgtcgattt tcgacatctg ctcatagcgg gcagtcaggc cgctactttg cgtctgcgcc
    30241 gcacgtaact ggttggtaat aaaagcatca tactcacgct gcacaccaga aacgtagaca
    30301 ccattgccaa cccagccgcc agcgcccaac gtgctattgg cctgcgccat aatagtggtt
    30361 tggcgggtat atccggcaac gttatagctg gagatattat tacttgccgt atttaacgcc
    30421 gcctgggccg cgttcagtcc gctcatggcg ttattgatca agctggacat ggaggttcct
    30481 tataagcctt caattacaga gtattatcgg cagcgacccg ccggacttga gttattcaga
    30541 acagattatc aatgttcatg ctgtaggttt tgctcacctt gtcgcttatc gatttcatct
    30601 gctggatcat gttggtgagt ttacgggcat agtgaggatc ggtagcatac cccgcgtcct
    30661 gtagggcctg cgccccctgc tccgcactcg cggcggtcgt cacggcggcg tagcgcgggt
    30721 tacgtgttaa cagcccaacg taatccgaca atgcttccag atacgagctg tagacccgaa
    30781 acttcgcttt tactttcttc gcttcgccgt tttcatattc agtcgtggtg atttcagtaa
    30841 ctggcccttt ccagttgcca gaggctttga caccaaacag gttatagctc ggctcgccgt
    30901 tttcgcggcg gatttgccgt tgcccccaac cagattccag tgccgcctga gcgaggatca
    30961 aatgatgtgg cacaccgctt tgctggcttg ccagttgggc gggcagcgag agttgcgcga
    31021 ggaatgcttt actgtcaccc ggcagcgaat catcgtagtt acgtggcacg gccttttgca
    31081 ccagctgcga aagcgcctga ttttgataac gcaccacagt ttcgagcggg aatttcatcg
    31141 gtgctgctgg cgtggactcc tctggcaatg gttgttctgg cgtcatctgt ttaaccatca
    31201 tctctgcaag ccccagacct ttgcccgccg tcatctgttg ggcaatctgc tggtcataca
    31261 tactggtata caggcgagtg tgctcgctgc tgaacaggcc atcttttggt aaagcgtcgc
    31321 gcatgctttt caacatcatc tgcacgaaca tcccttccac ctgacgggcc accggacgga
    31381 tatttgccgc cggatcttcg cccgctttcg cctttagttc gttgagtgat tgcgcatccc
    31441 aggccgcact tgccagtagt ttgctgtcgc tgatcatcag atgatttcca gttttgcccg
    31501 cagacatccc gcactttgca ttgattgcag tatggacatc agatccatcg gcgtagcgcc
    31561 cagcgcattg agcgcgcgca ccacgttatt gaggctggcg ctggaacgta cgctttgcag
    31621 cgaaccgccg ctctggcgta aatcgatctg cgtttgtgga gtaaccacag tctgtccacc
    31681 accaaacggt gtatctggct ggctgacatt ggcctgacga ttgactgtta ctgagagatt
    31741 tccctgcgct accgcgcagc tgtcgagggt cacttcgcga ttcatcacca ccgaaccggt
    31801 gcgcgagtta atcactactt tagcgtcctg cggggtgaca ttaacctgca tattctggat
    31861 atctgcaagg aaacggacct gggaactgtt gccacttggt acgcgcacct gaatagtccg
    31921 cgcatctaac gcggtggcgc tgccatatcc acgcacgcgg ttgatggtgt cagcgatttg
    31981 ctgcgccatg ctgaaatctt cgtcgttaag ttgcaaatta agggtattcc cgacgccaaa
    32041 ctggctgggc aattcacgtt caataaccgc accattggtg atccgtccac cgttcagttg
    32101 gttcacctga acactgctac cgccagcgga ggctcctgcg ccgccaacca gaatattgcc
    32161 ctgcgccagc gcatacacct gactgtcaac gcccttaagc ggcgtcatca acaacgtacc
    32221 tccacgcaag cttttggcat ttcccatgga agaaaccacc acatcgatgg tttgcccctg
    32281 acgtccaaac ggaggaagtg acgctgtcac cattaccgca gcgacgtttt ttagctgcat
    32341 attggtgccc gtcggaacgg taattcccag ctgtgagagc atgttattaa gcgtttgtgt
    32401 ggtaaacggc gtctgggttg tctggtcacc ggtgccatcc agccccacca ccagaccata
    32461 gccaatcagt gagttttgcc ttaccccctg aacactggtg agatcgcgaa tacgctcagc
    32521 ctgagccgcc gtcgtaacca gtagaagaat taatgcagag agaaatttaa tcaccacagc
    32581 ctcacttaca ttggcgacag gttaaggaag aaacgctgca accagcccat attttgcgct
    32641 tcgttaatgt agccattgcc tacgtattca atgcgcgcat ccgccacctg agtagacggt
    32701 acggtattgc tgccgctgat agtgcgtgga ttaaccacgc cagagaagcg aataaattcg
    32761 gtaccctgat taatggcaat ctgtttttca cccaccacat gcaggttgcc gttgaccagt
    32821 acctggtcaa ccgtcaccgt caacgtgccg ctaaaggtat tgctggcatt ggccccgccc
    32881 tttccgttga acgtgttacc accggaggct tcgacatcgg cacgagcgtt accaaacagc
    32941 ccctgcaaat agcgcggcac agtatcaaag ccaaaattag ttttaccgtc acggctggca
    33001 ttcgcagagg agcttttgct ggcgctgacg ttctcctgca acacgatggt cagcgtatcg
    33061 ccaatattgc gtggtcgacg atcttcaaac agcggttgat agccatagtt aatcggctga
    33121 gcagactgga aaatagaacc gttggcgacg ggcgtcggcc cgggaaccgg ttgtgcactg
    33181 gtcgccccct gcaccagcgg cgtggagggt atccaggcgc agccggttag tgaaagcacc
    33241 aacaagctgg aaatggcata agtatgcgca gcgttttttt gcatggctat catcttcaaa
    33301 aatcagtaaa ccggtgaacc tgccaccggt taagccttag agttgcgtca gtttttgcag
    33361 catctgatcg gtggtggaca ccgctttact gttgatttcg taagcgcgtt gcacctgaat
    33421 catattgacc agttcttccg ccacgttgac gttagacgtt tcaacatacc cttgatacag
    33481 cagtcccgcg ccgttcaggc ccggcgtgct ttcgttcggt gcaccagagg attgcgtttc
    33541 ggtgtagagg ttttcgccaa tgctctccag cccggtgtca ttcataaagg tggtgagatt
    33601 gagctgccca acctgaaccg gagctgcctg gccttgttgg gttacgctga ccacgccatc
    33661 acgaccgatg gtgatactta acgcattcgc cggaatggtg atcgctggct gcacctgaaa
    33721 accaccagcc gtcaccagct gcccgttctg atccacctgg aaagagccgt cacgggtata
    33781 ggctgatgaa ccatctggca acatcacctg gaaaaagccc tgccctttaa tcgcgacatc
    33841 tttgctgttg ttggtctgcg acaggtttcc ctggctgtgt aagcgttcag tggcgaccgg
    33901 gcgtaccccc gtgccgattt gtaatccgga gggtaaggtg gtttgttcgg aagactgtgc
    33961 ccccggctgg cgaatggttt gataaagcag atcttcaaac accgcgcgct gacgcttaaa
    34021 accgttagta ctgacgtttg ccaggttgtt ggcaatgacg tccatattgg tttgctgggc
    34081 gtcaaggccc gttttggcga tccataatga actgatcata aaatgtcctg tagtggatta
    34141 gtgacaagca gagcagaagc acgcgacagt gtgaatttcc cctcacccta accctctccc
    34201 caaaggggcg agggaatagt ccgcgcgctt ttattgcaat gtcatcaatc gttcaattaa
    34261 ctcatcgaca gcagttggtt ggcacggcct gcgttatcat cgacgctgct gatcaccttc
    34321 atctgcattt caaaacgccg cgcgctggca atcatgtcgc tcattgccgc aacggcattg
    34381 acgttactgc cttccagaac ccccgacatc acacgcaagg ttggatctgc ctgcagtatc
    34441 ggcccacgcg tggcctggct ttctgcgctt aaacgaaaaa tgccgtcgtc accgcgttgc
    34501 acttcgctgc ccgtggcttt caccagtttc agacgcccca ctggcgcaac cgtatttgcc
    34561 ggatcgcccg gattgagcgc cgagattgtg ccatcggcag caatagtgat ttccgcccct
    34621 tccggcacag caattggccc agcctcgcct atcaccggat gcccctgaat tgtcagttgc
    34681 ccggtgggat caacctgaat gctgccatta cgcgtatacc cttcgctgcc gtcagcggac
    34741 tgcacggcca gccagccatc ctgctgcaat gcaacgtcca gcgggcgcga ggtgtaatcc
    34801 atcttgccgg gcgtcatgtc tgcacccggt gttgacgccg ttaccagcgt gcgcgtgggc
    34861 agtgaaagcc cttccaccgg cactgcacgc aaggcgttta actgcgcgcg aaaaccgggc
    34921 gtagaggcat tggccagatt actggcggtt accgcctgtt gattcagcgt ctggctggct
    34981 gctcccatcg cggtataaat tgcgtgatcc attgagctat cccgtcagcg attagcgtaa
    35041 gttaaccagc gtgttgagga tctggtcctg ggttttgatg gtctgggcgt tagactgata
    35101 gttacgctgg gcaacgatca tattgaccag ttctttactg agatcgacgt tggacgcttc
    35161 caacgcaccg ttggtcaggg tgccaaagtt gcccgtcccg gctgtcccca acagcgccac
    35221 gccagaagat tgcgtcgcag accagacgtt gtcgccttcg gatgccagac cttcgttgtt
    35281 ggcaaagttc gccagtacaa tctgccccag cagttgtgtt tgttcgttgg aatagttgcc
    35341 gacaaccgta ccgtcatcat tgatttgata actcaccaga tcgcccggtt tgtagccatt
    35401 ctgggtggtt gccacaatat tgttagcgcc ggtattttgc tgcatggagt tgaggaagct
    35461 caggctaaac gtggcgggat ctgcgccgtt aattgcgcca gtggtaatat tctctgttgg
    35521 atttgaggtc agaacgccat tggcattaaa caccagcttc attgcaggct cggctgtacc
    35581 tgttggatca ctgctatcct gggtgtagac ctgccagtta ttatccccgg tcttcacaaa
    35641 gtagacgctc atgtcatgag cattaccctg actgtcgaaa acagtcaccg aacctttttt
    35701 gttatagcta tccgcattgc tggcgctgaa tggcgtaaca gtaggaagcg gatcactgga
    35761 attcaggttg atctgcatcg atgccgtggt ggtagttttc gctgccatca gggtattcgg
    35821 gatcgaaata ttggtcggat tcgccccttg ctgaatagtc ggcggcgtac cggttgccgg
    35881 gtaacccgtc agctgtaaac cttgcatatt caccaggtta cggttttcat ccagcttaaa
    35941 ttgtccgtta cggctgtaga acaccgaacc gttgctgtct accagacgga aaaaaccgtt
    36001 ctggctgata gcaacgtcca gacctcgccc ggtgttggtg gtcgtgccat cggtaaagtc
    36061 ctgagtgata ccggcaactt ttacccccag tcccactttc gaaccggcaa acatatcggc
    36121 aaaagaggcc gtgcctgatt taaagccgta ggtggcggag ttggcgatat tgttgccaat
    36181 aacatcgagg ttggtggcgg cagcgtttaa tccgctaacc gcttgagaaa aggccatgac
    36241 tgactcctga aatgtgaagg cttaaattat ctgccgtact tcgtcgaggg tggtggtgcc
    36301 gtaagtgccg agatccagcg tattaccgct gttgccgcgg atcacaccct gcaccagagc
    36361 aaactgcagc ggctgggcaa ccagttgtgt accaccgtta ctggcgctaa tcgctacatt
    36421 gtaagaaccg ttcggcgcag tgctgccgtc agtcaacgta ccgtcccagg tgaaactgtg
    36481 aactccagcg gtcagttcac caatatcaat ggtgcgcaca accgcaccat ttttatcggt
    36541 gatggtggcc gtaactttgt ctgccgcctg ttgcagctca acaccaaacg gcgtggtcgt
    36601 ggtcacagct ccttcttcac tgccggttcc cgcaagaaca gtggtgccgg ggatcatcac
    36661 gccgtgaccg atcaggttac tggcctgtaa cgactggctg ttatcaatct gtccggaaat
    36721 agatccgagt gtggtattga gtttttcaat cccgctgacc gtgctgattt gtgccaattg
    36781 cgacgtcagc tcgttgtttt ccattggatt ggtcgggtcc tggtttttca gctgcgccac
    36841 cagcaaagtc agaaaactgc tttgtaaatc tgcggcgttg ctgcccgtga gcgaactact
    36901 gctggtggta ctgacgccgg tatttgtcgg atcggtggtg gttaccgcaa tggacatagc
    36961 tttctccttt attgaccgag cgtaagggtt ttcagcatca tgcttttcac cgtgttgagc
    37021 acttcaacgt tggcctgata gctgcgtgac gccgacatgg tgttaaccat ctccccgacg
    37081 acatcaacgt tcggcatttt tacgtagccc tttgcatctg ccagcggatt acccggttca
    37141 taaaccagtt tgtccggggc ctgactttct ataacatcgg caacctttac gccgcctgtc
    37201 gcagcacctg gtgcagcgtt aacctggaat accacctgtt ttgcccgata tggctgtcca
    37261 tcaggaccgg tcacgctatc agcatttgcc agattactgg ccgccacgtt caggcgctgg
    37321 gactgggcag ttaacgccga cccggcgata tcaaaaatat tcagcagtgc catccgttaa
    37381 tttccgctct gtaaaacgtt catcatgcct ttgatttgcc cgctcaacgc gctaaggctc
    37441 atctggtatt gcaggctgtt atcggcaaac tgggtgcgtt cgcgatccat atcgacggta
    37501 ttaccgtcaa gcgaaggctg gtccggaata cggtattgca gttctgcggt aggaggcgtc
    37561 agcgcctgcg ccgggatgtg ttgcgttgag gtcatcgtca gtgcaaccac actggttgca
    37621 tcccgtccac gttgcatgac ttttttaagt tcactggcaa aatcgatatc gcgcgcctga
    37681 taaccagggg tatcggcatt ggcgatgttt gctgccagca cttcctgacg ctgggcgcgc
    37741 agattgagcg cctcttgttg aaaacgtaag gcggcgtcga gcttatcgag catatctcct
    37801 ccgcaggtat caaaattctg ccatcagctt aaatgccttt acgcgcgcgt tatcggcgga
    37861 ataaacgcaa aatgggtcgc tatttatgcc gttgatggtc attgcggaca ggtacaattc
    37921 acgttgtaga aatggctggg ggcgaaaatg ctgacaataa aacgtagcgt ggcgatcatc
    37981 gcgatactgt tcagtccgtt aagtacggcg agcaatctca catcgcaatt gcacaacttt
    38041 tttagcgccc aactcgcggg ggtaagtgat gaggttcgtg tttctattcg tacagcgccc
    38101 aatctactac cgccatgcga gcagccattg ctttcgatga gcaataattc ccgcctgtgg
    38161 ggcaatgtga atgtgttggc acgctgcggt aacgacaaac gatatttaca ggttaatgtg
    38221 caggccacag gaaattatgt ggttgccgcg atgcccattg tgcggggagg aaagctggaa
    38281 gctggcaatg tcaaactaaa acgcggacgg ctggataccc tgccaccgcg tacggtgctg
    38341 gatatcaatc aacttgttga tgccattagc ctgcgcgatc tatcacccga tcaacctatc
    38401 cagttaaccc agtttcgcca ggcatggcgg gtaaaagcgg gacaacgcgt caatgtgatc
    38461 gccagcggtg atgggtttag cgccaacgca gaaggtcagg cgctgaacaa tgcagccgtc
    38521 gcacagaatg cgcgggtgcg catggtatcg ggacaggtag tcagcggcgt tgttgatgca
    38581 gatgggaata ttcttataaa cctgtaagct gttaaagatt acccgtccct tgccgataaa
    38641 taagcaacac atgataaaag cgccctcaat gaggaataaa ccatgagtat tgatcgcact
    38701 tcgcctctga agcctgtaag caccgttcaa ccgcgcgaaa ccactgacgc gccggtaacg
    38761 aacagccggg cggcaaaaac aaccgcctcc accagcacca gtgtgacgtt aagcgacgcg
    38821 caagcaaaac tgatgcaacc cggcagcagt gatatcaatc ttgaacgtgt cgaagcgtta
    38881 aaactggcga ttcgtaacgg tgaactaaaa atggacaccg gcaaaattgc cgatgcgctg
    38941 atcaacgaag cgcagcaaga cttgcagagt aactgatcgt atgacacgtc ttgcagagat
    39001 cctcgaccag atgtccgctg tgcttaacga tctcaaaacg gtaatggatc aagagcagca
    39061 acatctctct atggggcaga tcaacggcag ccagttgcaa tggattacag aacaaaaaag
    39121 ctcactgctg gcgacgctgg attacctcga acagttacgc aagaaagaac ccaatacagc
    39181 aaatagcgtt gatattagtc aacgctggca ggaaattact gtgaaaacgc agcaactacg
    39241 ccaaatgaat caacataacg gctggttact ggaaggacag attgagcgca atcaacaggc
    39301 gctggaaatg ttgaaaccgc atcaggaacc gacgctatat ggggcgaacg gtcagacctc
    39361 aacaacccat cgcggcggta aaaagatttc gatctgaata ttatctccgg cctgcactgc
    39421 aggccggaat gcattgttac accgtccggc gggcaaattc tttaactttg aagcccagta
    39481 ccgccagtgc agcgaagtac gcggcaatcc ccgccagcac gaccgccatt aaacgcagta
    39541 agcgccaggg catggtaccc aatgaccact ccggcatgat atgtaacata cctaaaagca
    39601 cgccagacat caccagtacc gccatcacca gacgcaacag aaacgccatc cagccgggtt
    39661 gcggagtaaa gattttctgc ttacgcaact gccagtaaag cagcgaagca ttcagacacg
    39721 ccgccagacc aatagaaagt gacagcccgg catgtttcaa cggaccaata aacgccaggt
    39781 tcatcaattg cgttaaaatc agcgtaacga tggcaatttt cactggcgtt ttaatgtcct
    39841 ggcgggaata aaagccagga gccaacactt tcactacaat caggccaatc aaacccaccg
    39901 agtaggcaat taacgcccgc tgggtcatca gcgcatcaaa cgcggtaaat ttaccgtact
    39961 ggaacagcga aacggttaac ggaccagaaa gaatgcccaa cgcaaccgca ctcggcagcg
    40021 ccaacaggaa acaaagacgc aacccccagt ccatcaaacg gttgtattca tcatgattgc
    40081 cactggcaaa acttttcgac agcgacggca gcaaaatggt gccaagcgcc acgcccagca
    40141 caccggacgg aaactccatt aagcggtcgg cgtaatacat ccaagacacc gaaccggaag
    40201 caagaaacga ggcaaaaatg gtgttgatga ttaaggagat ctggctcaca gagacgccaa
    40261 ggatcgccgg tcccatctgt ttcaccacgc gcattgttcc ggcatcgtgg aagttaatgc
    40321 gcggcaggac cagcatgccg atcttcttca ggtgcggtag ctgatacacc agctgcagga
    40381 tgccgccgac cgtaacagcc cacgccagcg ccagcaccgg tgggttaaag tacggtgcgg
    40441 caaacagcgc gaaaccaatc atgctgatgt taagcagtgt tggagcaaac gccggaatcg
    40501 agaagcggtt ccacgtattc agaatcgctc ccaccagcga cgccagggag atcagcaaga
    40561 tatagggaaa ggtaatcttt agtagctggc tggtcagggc aaatttgtca gctgtgtcag
    40621 cgaagcctgg cgcggtcacc atgatcaccc acggtgcggc gagcatgcca gcgaccgtca
    40681 caaccgccag cgcgagtgtc agcaggccag aaacataaga gacaaagacc cgcgtggcgt
    40741 cttcaccctg cttactttta tattccgcca gaatcggtac aaatgcctgg gaaaatgccc
    40801 cttcggcaaa gatacggcgt aacaagttag gaagtttaaa agcgacgaaa aaggcgtcgg
    40861 ttgccatccc tgcgccaaag attctggcga caattgcgtc tcgtgcgaag ccaagcacac
    40921 gcgaaaacat ggtcatcgag ctgacggcgg ccagcgattt taataaattc atcggtgttc
    40981 taatccagac ccacgggcaa aacgcctgca aggcaggcgc tgaaagaggc gaatagtcta
    41041 cctggattat ggtgaattgc taccgccaga tgttacaggg tttattcact catcgcatcg
    41101 cgccagatct tgtcaacgat acgttgcgcc agcacggcct gttcgccggc ggtttgcgga
    41161 actgtctgat tttgcacaca ttcaatgaag tgccgcgcac agccgacaaa tccacgttgt
    41221 tcaagagtgc tctgccaacc aggaatcggt ttatgcacta cgccctgccc gcgctcctca
    41281 cgccattcgc gcatatccgt aatgtcgatg agcgcaccgt cagtcacggc ctgcacggtt
    41341 tcacgctgac ttccggcacg gcgatgcata caggtggtga tctgcaaagg accggccgaa
    41401 aaatggtgct cggcaaacag catttcgcca gcgtcgttag tcagtagcgt accgccatcc
    41461 agagaggctt tgccgcccga caaccacagc acggtatcca ccacatgcag ataatcatcc
    41521 agcaacgtga aataaagatc gtgtggcccg acactattgc tacgatgttt atccattctt
    41581 agcgaagctg cggtagcgag ttgcgttttt aactcaccgt agagtggtgc gaaacgacgg
    41641 ttaaaaccga ccatcaacgt cagtttttta cgcgccgcca gttccaccag ccgttcagca
    41701 tcgcgcagat tttctgccag cggtttatcg acacagacat gtacacccgc attgagtaac
    41761 gtactgacca cgtcaaagtg gctggcggtg ctggaatgca caaaaaccgc atcgcaactg
    41821 gcggcaaggc tggataacga atcggcataa ggaatgcgcc agctttcaca aattggcagg
    41881 gctttcgcgc gcgtaggcga ccaggctcct tgtaacgtcc agtcagacgc tgccgccagc
    41941 accggtaacc acgctttttg cgcaatgcca cctaatccca ctacgccgat acgtaatttt
    42001 ttcactttaa tctcccagat gggccagcaa cgaatcaaga cgctgtttca gttctgccac
    42061 ttcgatttcc agggcttcga cgcgggcctg taaatcacca tcaaccgcgt tcgacatatc
    42121 cgtcaccgcc ggctgatctt caacctcacc actgaaaaga tgcatgtagc ggctttcgcg
    42181 tttacccggt tcgcgggcca gacgcaccac aaaaggacca tcttcgcgat ttgccagttg
    42241 ttccagcgtc gactccactt ccgccatatc gctgaattca tacattcgcg cggcgcggct
    42301 gcgcagttca cctggcgtct gggcaccacg caataacaac gtggtgatta acgccacttc
    42361 cgctgcgctc agtttcagat cgccaaattc tgaattacaa aaacgttgct catatttggt
    42421 gacccgatta ccaaaaccgc tcactgtgcg tagataatga cgtttgacca gattatccag
    42481 ctgttcctgc acttcggatt cactcagatt catgaccggt tcacggttcg ttttctgatt
    42541 acaggccgtg actacaccat tgactgagag cgggtattgc tccggcgtgg tcacctgctt
    42601 ttccagcaag caaccaatca cgcgagcttc aagggcagta agttgatatt tcatgagatt
    42661 ctccttagcg gccgggcgtc cagtctgggg tagttaatgc cgtcagtacg tgatcgcgcc
    42721 attgtccatc aatcaacaga tagtctttcg catagccttc tttttcaaaa cccagtcgcg
    42781 ccagtaaatc accgctgcgt ttattgtgcg gcatataatt agccataatg cgatgaatat
    42841 gttgggtgcg ctgcatataa cgaatggctg cggtcagggc ttcaaacatg agtcctttgc
    42901 cctgccattt ttgcccaatc gaataaccga gatagcaggc atgaaaagag ccacgaacaa
    42961 cattggaaaa attggcaacg ccaataatct ctttttcgtc cgggtcgaat aagccaaagt
    43021 agaaagctga accttgttta tgaaattcgt taatcatccc cagcctggcc tgccagcctg
    43081 atggataaca gtggctttcg tcgcgcactg gctcccaggg cttgaggaaa tggcgattct
    43141 ctgcgtaata atccgcaaga cgccaggcat cacgatcatg caccagacgc acgaccagtc
    43201 ggtctgtggt taagcgcact tttggcacgt tactgcgata gccaaacatt ctatacctac
    43261 tccttcccgt aacgtcttta atacgctgta agtattacta tacctgcgca accagaccct
    43321 gtgaaaagtc caaaatgcca ttttctgcat tattcacata tttgataaaa actctctatc
    43381 aaagcgacgt tttgataaaa aaatattgtc cccgaagtga tgggaaaagt cttagcggca
    43441 atgcagaata gaaagtgctc atcttttatt tcccgggagg ggaaatgtcc cgcgtatcgc
    43501 aggcgaggaa cctgggtaaa tatttcctgc tcatcgataa tatgctggtc gtgctggggt
    43561 tctttgttgt cttcccgctg atctctatcc gcttcgttga tcaaatgggc tgggccgccg
    43621 tcatggtcgg tattgctctc ggtctacgcc agtttattca gcaaggtctg ggtattttcg
    43681 gcggcgcaat tgccgaccgc tttggtgcca aaccgatgat tgttaccggt atgctgatgc
    43741 gcgccgccgg attcgccaca atgggtatcg cccacgaacc gtggctattg tggttttcat
    43801 gcctgctctc gggactcggc ggcacgttgt ttgatccgcc gcgttcggcg ctggtggtga
    43861 aactaatccg tccacaacaa cgtggtcgtt ttttctcgct gttgatgatg caggacagtg
    43921 ccggtgcggt cattggcgca ttgttgggga gctggctgtt gcaatacgac tttcgcctgg
    43981 tctgcgccac aggggccgtt ttgtttgtgc tgtgtgcggc gttcaatgcg tggttgttac
    44041 cggcatggaa actctccacc gtacgcacgc ccgttcgcga aggcatgacc cgcgtgatgc
    44101 gtgacaagcg ttttgtcacc tatgtcctga cgctggcggg ttactacatg ctggctgtac
    44161 aagtgatgct gatgctgcca attatggtca acgacgtggc tggcgcaccc tctgccgtta
    44221 aatggatgta tgccattgaa gcgtgtctgt cgttaacgtt gctctaccct atcgcccgct
    44281 ggagtgaaaa gcattttcgt ctggaacacc ggttgatggc tgggctgttg ataatgtcat
    44341 taagcatgat gccggtgggc atggtcagcg gcctgcaaca acttttcacc ctgatttgtc
    44401 tgttttatat cgggtcgatc attgccgagc ctgcgcgtga aaccttaagt gcttcgctgg
    44461 cggacgcaag agctcgcggc agctatatgg ggtttagccg tctgggtctg gcgattggcg
    44521 gcgctattgg ttatatcggt ggtggctggc tgtttgacct gggcaaatcg gcgcaccagc
    44581 cagagcttcc gtggatgatg ctgggcatta ttggcatctt cactttcctt gcgctgggtt
    44641 ggcagtttag ccagaaacgc gccgcgcgtc gtttgcttga acgcgacgcc tgatttgata
    44701 ctcccccctg actcctccat actgaaagtg aactgacagc caaatggagg agtcacgtga
    44761 agctatacat ttacgatcac tgcccttact gcctcaaagc ccgcatgatt ttcggcctga
    44821 aaaatatccc cgtcgaatta catgttctgc tcaacgacga cgcagaaaca cccccccgga
    44881 tggtcggtca aaaacaggtt cccattctgc aaaaagatga cagccgctat atgccagaaa
    44941 gcatggacat cgttcactat gtcgataaac tcgacggcaa accgttactg accggcaaac
    45001 gttcccctgc cattgaagag tggctgcgca aggtcaatgg ctacgcaaac aaactgctgt
    45061 tgccgcgttt tgccaaatcg gcatttgatg agttttctac tcccgccgcg cgcaaatatt
    45121 tcgtcgacaa gaaagaggcc agcgcgggta atttagccga cctgctggcc cactctgacg
    45181 gtctgattaa gaatatcagc gatgatttgc gtgcgctgga caaactgatc gtcaaaccga
    45241 acgccgtgaa tggcgaactt tcggaagatg atattcagct atttccgcta ctgcgtaatc
    45301 tgacgctggt agccggaatc aactggccaa gccgcgttgc tgattaccgc gataatatgg
    45361 cgaaacagac acaaatcaat ttgttatcat caatggcgat ttaatcctga cccgccgcgg
    45421 cagcctgtat tcgcgggttg ccgccgtttt cagttttttc tgctggcgag tttatcgctg
    45481 gtgacacatg ctgcgttgtg tcaggatatt gacgcagatt tcatgtcgag gactaccatg
    45541 aacaagtttt tatttgccgc tgcattgatc gtcagtggcc tgctcgttgg ctgtaatcaa
    45601 ctcacccaat acaccatcac cgaacaagaa attaaccagt cgcttgcgaa acataataat
    45661 ttctcaaaag atatcggttt acccggcgtg gctgacgccc atattgttct gacaaacttg
    45721 accagccaaa ttggtcgcga agagccgaat aaggttaccc taaccggaga cgccaatctg
    45781 gacatgaact ccctgttcgg tagtcagaaa gcgaccatga aactgaagct gaaagcgctg
    45841 cccgtgtttg ataaagaaaa aggtgcgatc ttcctgaaag agatggaagt ggtcgatgcg
    45901 acggtacaac cggaaaaaat gcaaacggtg atgcaaacgt tgcttcccta tttgaaccag
    45961 gcattacgca attactttaa ccagcaacct gcttacgtcc tgcgcgaaga tggcagtcag
    46021 ggcgaagcaa tggcgaaaaa actggcgaaa ggcattgaag tgaagccagg cgaaattgtc
    46081 attccgttta cggattaatg acgagggcgc attcgcgccc tttatttttc gtgcaaagga
    46141 aaacgtttcc gcttatcctt tgtgtccggc aaaaacatcc cttcagccgg agcatagaga
    46201 ttaatgactg caccatccca ggtattaaag atccgccgcc cagacgactg gcaccttcac
    46261 ctccgcgatg gcgacatgtt aaaaactgtc gtgccgtata ccagcgaaat ttatggacgg
    46321 gctattgtaa tgcccaatct ggctccgccc gtgaccactg ttgaggctgc cgtggcgtat
    46381 cgccagcgta ttcttgatgc cgtacctgcc gggcacaatt tcaccccatt gatgacctgt
    46441 tatttaacag attcgctgga tcctaatgag ctggagcgcg gatttaacga aggcgtgttc
    46501 accgctgcaa aactttatcc ggcaaacgca accactaact ccagccacgg cgtgacgtca
    46561 attgacgcaa tcatgccggt acttgagcgc atggaaaaaa tcggtatgcc gctactggtg
    46621 catggtgaag tgacacatgc agatatcgac atttttgatc gtgaagcgcg ctttatagaa
    46681 agcgtgatgg aacctctgcg ccagcgcctg actgcgctga aagtcgtttt tgagcacatc
    46741 accaccaaag atgctgccga ctatgtccgt gacggaaatg aacggctggc tgccaccatc
    46801 actccgcagc atctgatgtt taaccgcaac catatgctgg ttggtggcgt gcgtccgcac
    46861 ctgtattgtt tacccatcct caaacgcaat attcaccaac aggcattgcg tgaactggtc
    46921 gccagcggtt ttaatcgagt attcctcggt acggattctg cgccacatgc acgtcatcgc
    46981 aaagagagca gttgcggctg cgcgggctgc ttcaacgccc caaccgcgct gggcagttac
    47041 gctaccgtct ttgaagagat gaatgctctg cagcactttg aagcattctg ttctgtaaac
    47101 ggcccgcagt tctatggctt gccggtcaac gacacattca tcgaactggt acgtgaagag
    47161 caacaggttg ctgaaagcat cgcactgact gatgacaccc tggtgccatt cctcgccggg
    47221 gaaacggtac gctggtccgt taaacaataa aaatcgtagc ttcctgttgt cattaggtta
    47281 ttttacctgt ataaataacc agtatattc
//
LOCUS       DAZGJW010000019        45110 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZGJW010000019 DAZGJW010000000
VERSION     DAZGJW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.019"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            65..649
                     /gene="yadN"
                     /locus_tag="ACHKIK_001144"
     CDS             65..649
                     /gene="yadN"
                     /locus_tag="ACHKIK_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9082358.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            754..1494
                     /gene="yadV"
                     /locus_tag="ACHKIK_001145"
     CDS             754..1494
                     /gene="yadV"
                     /locus_tag="ACHKIK_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO9082359.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1529..4129
                     /gene="htrE"
                     /locus_tag="ACHKIK_001146"
     CDS             1529..4129
                     /gene="htrE"
                     /locus_tag="ACHKIK_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFO9082360.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            4146..4715
                     /gene="yadM"
                     /locus_tag="ACHKIK_001147"
     CDS             4146..4715
                     /gene="yadM"
                     /locus_tag="ACHKIK_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9082361.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLSGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            4730..5332
                     /gene="yadL"
                     /locus_tag="ACHKIK_001148"
     CDS             4730..5332
                     /gene="yadL"
                     /locus_tag="ACHKIK_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO9082362.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            5359..5955
                     /locus_tag="ACHKIK_001149"
     CDS             5359..5955
                     /locus_tag="ACHKIK_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO9082363.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            5939..7282
                     /locus_tag="ACHKIK_001150"
                     /pseudo
     CDS             5939..7282
                     /locus_tag="ACHKIK_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            7395..8189
                     /gene="panB"
                     /locus_tag="ACHKIK_001151"
     CDS             7395..8189
                     /gene="panB"
                     /locus_tag="ACHKIK_001151"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO9082364.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITDALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8201..9052
                     /gene="panC"
                     /locus_tag="ACHKIK_001152"
     CDS             8201..9052
                     /gene="panC"
                     /locus_tag="ACHKIK_001152"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO9082365.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIIRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9126..10028)
                     /gene="rpnC"
                     /locus_tag="ACHKIK_001153"
     CDS             complement(9126..10028)
                     /gene="rpnC"
                     /locus_tag="ACHKIK_001153"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO9082366.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            10302..10682
                     /gene="panD"
                     /locus_tag="ACHKIK_001154"
     CDS             10302..10682
                     /gene="panD"
                     /locus_tag="ACHKIK_001154"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO9082367.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10686..11915)
                     /gene="yadE"
                     /locus_tag="ACHKIK_001155"
     CDS             complement(10686..11915)
                     /gene="yadE"
                     /locus_tag="ACHKIK_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO9082368.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11979..12419)
                     /gene="yadI"
                     /locus_tag="ACHKIK_001156"
     CDS             complement(11979..12419)
                     /gene="yadI"
                     /locus_tag="ACHKIK_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO9082369.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDLAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12524..13294)
                     /gene="yadH"
                     /locus_tag="ACHKIK_001157"
     CDS             complement(12524..13294)
                     /gene="yadH"
                     /locus_tag="ACHKIK_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9082370.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13291..14217)
                     /gene="yadG"
                     /locus_tag="ACHKIK_001158"
     CDS             complement(13291..14217)
                     /gene="yadG"
                     /locus_tag="ACHKIK_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9082371.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14326..14988
                     /gene="can"
                     /locus_tag="ACHKIK_001159"
     CDS             14326..14988
                     /gene="can"
                     /locus_tag="ACHKIK_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO9082372.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15029..15565)
                     /gene="hpt"
                     /locus_tag="ACHKIK_001160"
     CDS             complement(15029..15565)
                     /gene="hpt"
                     /locus_tag="ACHKIK_001160"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO9082373.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15771..18161
                     /gene="gcd"
                     /locus_tag="ACHKIK_001161"
     CDS             15771..18161
                     /gene="gcd"
                     /locus_tag="ACHKIK_001161"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO9082374.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18208..19758)
                     /gene="cueO"
                     /locus_tag="ACHKIK_001162"
     CDS             complement(18208..19758)
                     /gene="cueO"
                     /locus_tag="ACHKIK_001162"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO9082375.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIILPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            19924..20271
                     /gene="yacC"
                     /locus_tag="ACHKIK_001163"
     CDS             19924..20271
                     /gene="yacC"
                     /locus_tag="ACHKIK_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO9082376.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20377..21243
                     /gene="speE"
                     /locus_tag="ACHKIK_001164"
     CDS             20377..21243
                     /gene="speE"
                     /locus_tag="ACHKIK_001164"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO9082377.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21259..22053
                     /gene="speD"
                     /locus_tag="ACHKIK_001165"
     CDS             21259..22053
                     /gene="speD"
                     /locus_tag="ACHKIK_001165"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO9082378.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22091..22453)
                     /gene="yacL"
                     /locus_tag="ACHKIK_001166"
     CDS             complement(22091..22453)
                     /gene="yacL"
                     /locus_tag="ACHKIK_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO9082379.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22628..25225)
                     /gene="acnB"
                     /locus_tag="ACHKIK_001167"
     CDS             complement(22628..25225)
                     /gene="acnB"
                     /locus_tag="ACHKIK_001167"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO9082380.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHSL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25580..27337
                     /gene="yacH"
                     /locus_tag="ACHKIK_001168"
     CDS             25580..27337
                     /gene="yacH"
                     /locus_tag="ACHKIK_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO9082381.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(27408..28832)
                     /gene="lpdA"
                     /locus_tag="ACHKIK_001169"
     CDS             complement(27408..28832)
                     /gene="lpdA"
                     /locus_tag="ACHKIK_001169"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO9082382.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29157..31049)
                     /gene="aceF"
                     /locus_tag="ACHKIK_001170"
     CDS             complement(29157..31049)
                     /gene="aceF"
                     /locus_tag="ACHKIK_001170"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO9082383.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31064..33727)
                     /gene="aceE"
                     /locus_tag="ACHKIK_001171"
     CDS             complement(31064..33727)
                     /gene="aceE"
                     /locus_tag="ACHKIK_001171"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO9082384.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33888..34652)
                     /gene="pdhR"
                     /locus_tag="ACHKIK_001172"
     CDS             complement(33888..34652)
                     /gene="pdhR"
                     /locus_tag="ACHKIK_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO9082385.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            35193..36566
                     /gene="aroP"
                     /locus_tag="ACHKIK_001173"
     CDS             35193..36566
                     /gene="aroP"
                     /locus_tag="ACHKIK_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO9082386.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAMIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(36609..37463)
                     /gene="ampE"
                     /locus_tag="ACHKIK_001174"
     CDS             complement(36609..37463)
                     /gene="ampE"
                     /locus_tag="ACHKIK_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO9082387.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPRAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(37460..38011)
                     /gene="ampD"
                     /locus_tag="ACHKIK_001175"
     CDS             complement(37460..38011)
                     /gene="ampD"
                     /locus_tag="ACHKIK_001175"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO9082388.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            38099..38992
                     /gene="nadC"
                     /locus_tag="ACHKIK_001176"
     CDS             38099..38992
                     /gene="nadC"
                     /locus_tag="ACHKIK_001176"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO9082389.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            39195..39635
                     /gene="ppdD"
                     /locus_tag="ACHKIK_001177"
     CDS             39195..39635
                     /gene="ppdD"
                     /locus_tag="ACHKIK_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO9082390.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            39645..41030
                     /gene="gspE"
                     /locus_tag="ACHKIK_001178"
     CDS             39645..41030
                     /gene="gspE"
                     /locus_tag="ACHKIK_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO9082391.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            41020..42222
                     /gene="hofC"
                     /locus_tag="ACHKIK_001179"
     CDS             41020..42222
                     /gene="hofC"
                     /locus_tag="ACHKIK_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO9082392.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(42257..43300)
                     /gene="guaC"
                     /locus_tag="ACHKIK_001180"
     CDS             complement(42257..43300)
                     /gene="guaC"
                     /locus_tag="ACHKIK_001180"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO9082393.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(43456..43500)
                     /locus_tag="ACHKIK_001181"
     CDS             complement(43456..43500)
                     /locus_tag="ACHKIK_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9082394.1"
                     /translation="MIIKTLKMSARKRN"
     gene            43525..44145
                     /gene="coaE"
                     /locus_tag="ACHKIK_001182"
     CDS             43525..44145
                     /gene="coaE"
                     /locus_tag="ACHKIK_001182"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO9082395.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            44145..44888
                     /gene="zapD"
                     /locus_tag="ACHKIK_001183"
     CDS             44145..44888
                     /gene="zapD"
                     /locus_tag="ACHKIK_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO9082396.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            44898..45095
                     /gene="yacG"
                     /locus_tag="ACHKIK_001184"
     CDS             44898..45095
                     /gene="yacG"
                     /locus_tag="ACHKIK_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO9082397.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
BASE COUNT        11281 a        11716 c        10995 g        11118 t
ORIGIN      
        1 gtgtcatgaa tagtcattct tcaaaccagt agtctgacta atggaatttt attcaaggat
       61 ttttatgtct aaaaaattag gttttgccct gtctggtctt atgctggcaa tggttgcagg
      121 tactgcttct gctgatatgg atggtggtca gttaaatatc agtggtctgg ttgttgataa
      181 cacctgtgaa acccgcgttg acggcggtaa taaagatggt ctgatcctgc tgcaaactgc
      241 aactgttggt gaaatcgatg ctggcgtgct gaatgacacc gttggcgcta aagccaaacc
      301 tttcagcatc accgttgact gcagcaaagc aaatccgaat ccaggtagta cagctaaaat
      361 gaccttcggt tctgttttct ttggtaatag caaaggaacg ctgaacaacg acatgtctat
      421 taacaacccg tctgacggcg tgaatatcgc tctgcacaat attgatggtt ccactatcaa
      481 acaggttcaa attaacaacc ctggcgatgt gtatactaaa gccctggatg caacgacaaa
      541 atctgctgtt tatgatttta aagcgtctta cgttcgtgcc gtcgcagacc aaacagcaac
      601 tgctggttat gtaaaaacta acactgcata caccattact tatcagtaag tattcaccaa
      661 ttaaattgat tgaatgaata tacagggaat agtgttaata atttctattt ttatattatt
      721 ccctgtttta attaactctg tcagggatgg tttatgtttt ttaacaccaa acatacaaca
      781 gctttatgct tcgtaacctg tatggctttt agttcatcgt ctattgcgga cattgtcatt
      841 tcgggtactc gcgtaatata taaaagcgat caaaaaagtg tcaacgtacg tctggaaaat
      901 aaagggaata acccgttgct tgtccagagt tggttagata ctggcgatga caacgctgag
      961 cctggcagta ttacagtccc ttttactgcc acgccgccag tatcgcgtat tgatgccaaa
     1021 cgtgggcaaa caatcaaatt aatgtacaca gccagcacct cactgcctaa agacagagag
     1081 agcgtgttct ggtttaacgt actggaagtt ccaccaaaac cagatgcaga aaaggtcgcg
     1141 aatcaaagtc tgctgcaact ggcatttcgc acacgtataa aacttttcta tcgcccggat
     1201 ggattgaagg gaaatccctc tgaagccccg ttagccctga agtggttctg gtcaggttca
     1261 gaaggtaagg cgtcattgcg agtgaccaat ccaacccctt actacgtctc ttttagcagt
     1321 ggtgatttag aagctagcgg taaacgctat ccgattgatg tgaaaatgat tgcaccattt
     1381 agtgatgagg tcatgaaagt cactggcctt aatggcaaag caagttctgc aaaagtacat
     1441 ttttacgcca ttaatgattt tggtggcgcc attgaaggta atgccagtct gtaatcaggc
     1501 aaggatataa ttccgcagga agcataacgt gactataaaa ccaactaaaa attatcataa
     1561 tcatctgacc cgtatcgcta cgttttgcgc gctgctgtat tgcaatagtg ctttctgtgc
     1621 tgaacttgtt gaatatgacc ataccttcct gatggggcag aatgcctcaa atattgatct
     1681 cagccggtat agtgaaggta accctgctat accgggtatg tacgacgtca gtgtttatgt
     1741 caacgatcaa cctattatta accaaagcat tacatttatt gaaattgaag gaaaaaaaaa
     1801 cgcacaagcc tgtatcacac ttaagaattt attgcagttt catataaatt ctcccgatat
     1861 aaataacgaa aaagccgttc tgcttgccag ggatgaaaca ctcggcaatt gccttaattt
     1921 gacggaaatt atccctcagg cttctgttcg ttatgacgtt aacgagcaac gtctggatat
     1981 agacgttcct caggcatggg taatgaaaaa ttaccaaaac tatgttgatc catcgttatg
     2041 ggaaaatggc attaatgcgg ccatgttgtc atacaacctc aacggatatc atagtgaaac
     2101 tcctggtcga agaaatgaca gcatttatgc tgcttttaac ggtgggatga atttaggtgc
     2161 atggcgactg cgtgcctcag gcaactacaa ctggatgacc gattctggca gtaattatga
     2221 ttttaagaat cggtatatac agcgtgatat cgcctcgctg cgttctcaac tcattcttgg
     2281 tgagtcttat acaacgggcg aaacctttga ttctgtcagt atccgcggca ttcgtttata
     2341 cagcgacagc cgcatgttgc ctccgacatt agcgagtttt gcgcctatca ttcatggcgt
     2401 tgctaatacc aacgccaaag tcactattac gcaaggtggc tataagattt atgaaacgac
     2461 tgtgccgcca ggcgctttcg tcattgatga tctgagtccg tcaggttacg gcagcgatct
     2521 tattgttact gtcgaagaat ccgatggctc aaagcggaca ttctcgcaac ctttttcatc
     2581 ggtcgttcaa atgttacgcc ctggcgttgg acgttgggat attagcggcg gccaggtctt
     2641 aaaagatgat attcaggatg agcctaattt atttcaggcc agctactact acggtctgaa
     2701 taactatcta acgggttata ctggtattca gattaccgat aataactata ccgctggttt
     2761 gttaggtctt ggtctgaata cttcagtcgg tgcattttct ttcgatgtga ctcactccaa
     2821 tgttcgtata ccggatgata aaacatatca gggacaaagt tatcgtgttt cctggaacaa
     2881 gttattcgaa gaaacaagta cttcactgaa tatcgcggcc tatcgctatt cgacacagaa
     2941 ttaccttggt cttaatgatg cactaactct aattgatgaa gtgaaacatc ccgaacaaga
     3001 tcttgaaccg aaatccatgc gtaattactc gcgcatgaaa aatcaggtta cggtcagtat
     3061 taaccaaccg ctaaaatttg agaaaaaaga ttacggttca ttttatcttt ccggaagttg
     3121 gtccgattac tgggcttccg gacaaaatcg tagcaattac tctattggct acagtaacag
     3181 tgcatcctgg ggcagctaca gtgtcagtgc ccagcgttca tggaatgaag acggcgacac
     3241 tgacgatagc gtttatctca gtttcaccat tcctattgaa aaattacttg gcactgaaca
     3301 acggacttca ggtttccaga gtattgatac tcaaatgagc agtgacttta agggtaataa
     3361 ccaactcaac gtcagtagca gtggctatag tgataacgct cgcgtcagtt atagcgtaaa
     3421 tactggctat acgatgaata aagccagcaa agatttgagt tatgttgggg gttatgccag
     3481 ctatgaatca ccgtggggaa ccctggcagg ttcagtttct gcaaatagcg ataacagtcg
     3541 tcaagtgtct ctcagcaccg acggtggttt tgtattgcat agcggcggcc tgaccttcag
     3601 taatgacagt tttagcgact ccgatacact ggcggtagtt caggctccag gtgctcaagg
     3661 agcgcgaata aattatggca acagtactat cgatcgatgg ggttatggtg tcaccagcgc
     3721 tctttctcct tatcatgaaa accgtatcgc gctggatatc aacgatcttg agaacgatgt
     3781 tgaattaaaa agtaccagtg cagtagctgt accgcgtcag ggttcagtcg tctttgctga
     3841 ttttgaaacc gtgcaagggc aatcagccat tatgaacatc acacgaagtg atggtaaaaa
     3901 tattccattt gctgcagata tttatgatga gcaaggcaat gtcattggta atgttggaca
     3961 gggtggacaa gcatttgttc gtggtattga gcagcaggga aatatcagca ttaaatggct
     4021 cgaagaaagt aaacccgtaa gttgtcttgc gcattatcaa caaagttcag aagcagaaaa
     4081 aatagcgcaa tctattattc tgaatggaat tcggtgtcag attcagtaac tacaagggac
     4141 gtcaaatgat aaaaataacg ccacataaaa tagcgatact gatgggaata ttattatcac
     4201 catcagtatt tgcaacggat attaatgtag agtttacagc cactgtcaaa gcgacaacct
     4261 gtaacatcac acttactggt aataacgtca cgaatgatgg caataataac tacacattga
     4321 gaatccctaa gatgggtctg gataagatcg cgaataaaac gacagaatct caggctgatt
     4381 ttaaactggt tgctagtggg tgcagcagtg gcatcagttg gattgatacc actctgagcg
     4441 gaaatgcatc atcaagctca cctaagctta ttataccgca gtctggtgat tcatcttcga
     4501 cgacaagtaa tatcggtatg ggtttcaaaa aacggactac tgatgatgcc actttcctta
     4561 aacctaacag tgcggaaaag atacgctgga gcacagacga gatgcagccc gataagggtc
     4621 ttgaaatgac cgttgcgcta cgtgaaacag atgcagggca aggcgtaccg gggaattttc
     4681 gtgcactggc aacgtttaat ttcatctatc aataagaagg ataattaaga tgacatttaa
     4741 aaatttacgt tatggattat ccagcagtgt cgttttggct gcctcattgt tcagcgtacc
     4801 ctcttatgcg gcaacagaca gcattgggct gaccgtgatt actaccgtag aaataggtac
     4861 ttgtaccgct acattagtaa atgactctga tcaggacatt tctgttgttg actttggcga
     4921 tgtatatatt tctgaaatca atgccaagac caaagttaaa acattcaaac tcaaattcaa
     4981 agactgtgcg ggtatcccca ataaaaaagc acaaataaaa ttaaccaagc gagccacatg
     5041 cgaaggtact gctaatgacg gtgcggggtt cgcaaatgga tccacagccg cagataaagc
     5101 aagtgccgtc gccgttgaag tctggagcac cgaaactccg gcaacaggga gcgcaacaca
     5161 atttagttgt gtgacaccag catctcaaga ggtcactatc tccaatgcag ccaacgcggt
     5221 tgtttattat ccgatgagtg ctcgactggt cgtcgaaaaa aataaaaccg taagcaatgt
     5281 cactgcgggt aaattttctg cacctgccac atttacagta acctataact aacagactga
     5341 cagatccagg aactatatat gcatcccact caacgtaagc tgatgaagag aataattctg
     5401 tttctgtcac tacagtttcc cattacctat ccagccatcg ctgggcagga tattgacctt
     5461 gttgccaatg taaaaaacag cacttgcaaa agcggaatca gtaaccaggg taatattgat
     5521 cttggcgtcg ttggggtggg atatttttca gataatgtta ctcctgaaag ctatcaacca
     5581 ggtggaaaag agttcactgt cacagtatca gactgtgcat tacagggaac tggcgatgtg
     5641 cttaatcagt tacatattga ttttagagcc cttagcggtg tcatggctgc aggttcaagg
     5701 caaatatttg ccaatgaagt cgcaaccggg gctaaaaatg tcggtgtcgt tctcttttcc
     5761 attcaggacc cgaccaatat attcaatgtt attagttcag caggaaattc tcgttccgtg
     5821 tatccagtaa tgacttctgc cctgcataat tcttcatgga aattttatgc cagaatgcaa
     5881 aaaatcgatc ccgcgctgga tgtcatatct ggccaggtaa tgagtcatat attagttgat
     5941 gtgtactacg aataatagat caaaacaaat aactgcgact gttacagcag tagatggtgg
     6001 agtgataatg aagaaattct tcagacattt tcttttttta attctgtgct tgagttgtta
     6061 taccgcgagt gctggcactg atgataacgt aggatatatt gttgggaata gttacggcgt
     6121 tggtccgtca gatcaaaaat ggagagaaac ggggccgaat ggcgatgcta ctgtcatatt
     6181 cagatatgca acaagtacaa ataatttagt tttctacaaa ccgacgcagc ttggacctac
     6241 aggtgtaaaa ttacagtgga gtcagttaga taccgcttct ggtggtggtt ttctttattg
     6301 caacagaagt gatagcacaa gtggtagcgc aatgcgtatt gaaaatgcaa tggttgactc
     6361 aggtaaaatg tatggctccc ataaattatt taatacatca gttcctggtt tgtattacac
     6421 attattaatt tcaaacatgt ggtcagctta cggtaccgta actaacgtta gttcacctgg
     6481 gatatacatt ggtgactctg cagaacaata tttttcgtgg tataatccaa gcgaagacgt
     6541 gttatattag agttgcaata atgcgaatag cacccgtaaa tactgggctg taggtggtat
     6601 ttatcagacc cttacaattg aattctatac agatacaaac tttgatccaa cagtaactca
     6661 acagatcaaa ttatccagct catcaaatta tctgtattca tttaaagcct atggcgcagg
     6721 ccaaggtata aatgagcata gttattttat caaaatcgat ttcgatctgc tcaatgtcaa
     6781 gttgactaat cccacttgtt ttaccgctat gcttagtgga acttctgtga ctggttctac
     6841 ggtaaaaatg ggtgaatata gtgcagaaca aatcagaaac ggtgccacac cggttccttt
     6901 tgatatttca cttcaaaact gcgttcgtgt gactaatatt gagacaaaat tagtttcaac
     6961 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgacgcagc
     7021 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
     7081 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
     7141 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
     7201 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
     7261 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcagtaa
     7321 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
     7381 tatcaggata cgttatgaaa ccaaccacca ttgcttcact tcagaaatgt aagcaggata
     7441 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
     7501 aaggacttaa cgtcatgctg gtaggcgact cgctgggcat gacggttcag ggtcacgact
     7561 ccaccctgcc ggtgaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
     7621 cgccaaactg cctgctgctg gctgatctgc cgtttatggc gtatgccacg ccggaacaag
     7681 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
     7741 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
     7801 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagtt caggggcgcg
     7861 gcgatgaagc gggcgatcaa ctgctcagcg atgcattagc cttagaagcc gctggggcac
     7921 agctgctggt gctggaatgc gtgccggttg aactggcaaa acgtattacc gatgcactgg
     7981 cgatcccggt tattggcatt ggcgcaggca acgtcactga cgggcagatc ctcgtgatgc
     8041 acgacgcctt cggcattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     8101 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     8161 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     8221 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     8281 gcctactatg ggtaatctgc acgatggcca tatgaagctg gtcgacgaag ccaaagcccg
     8341 cgccgatgtg gtcgtcgtca gtattttcgt taacccaatg cagtttgacc gcccggaaga
     8401 tctggctcgt tatccacgga ccttgcaaga ggactgcgaa aagctaaaca aacgtaaagt
     8461 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     8521 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     8581 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     8641 cttcggtgaa aaggactttc agcaactggc gctaatccgc aaaatggttg ccgatatggg
     8701 cttcgatatt gaaattgtcg gcgtcccgat tatacgcgcc aaagatggcc ttgcgctcag
     8761 ttcccgtaat ggttatctga cggcggaaca acgcaaaatt gcgcccggtc tgtacaaagt
     8821 tttaagttca attgccgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     8881 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     8941 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     9001 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     9061 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     9121 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     9181 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     9241 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     9301 gtcatcatag actcccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     9361 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     9421 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     9481 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     9541 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgtgccagc
     9601 tccggcgagt aaaatatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     9661 tgataaaaca gtatcggcac caccagcggc agcttatcgt gatccgcctc cagatgccgg
     9721 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     9781 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcac ggaatagagc
     9841 acgtccgtgc tgtgtccttt caggctctct tcaatgaaac tccccgactc taaatgaagc
     9901 gtgttgaggt cacaaagttc tcgtaattcc actggcaaat gtatctccag aaagtcgcga
     9961 gccgtctccg cgtgcattaa aaattgttta aacactgcat catgcggtgt ggtactcggt
    10021 gcatccatct tccctgcctt ccgtgttgtc ttgcgtgaag gcagtatcca gtcaaggcgt
    10081 gccatcgtca gccacctctt tagtgtttac cgagcagcgt tcagagaaat ttcaattggt
    10141 tacagccgtt acatggcacg cttcggcacg gtacgcaaac tgctgatgta aatgccgtca
    10201 gatgagtaga cactaaacaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
    10261 atcgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
    10321 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
    10381 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc attgatatct
    10441 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
    10501 gaattatttc tgttaacggt gcggcggccc actgcgccag tgtcggcgat attgtcatca
    10561 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aacgtcgcct
    10621 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
    10681 gattgttatc cctgcggctg gttactcacc agccgcgaca tcgtctccag cgaatccgtt
    10741 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
    10801 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
    10861 aatccgccaa acggatacga aagataccag acatgcggat taaattgcgc cagagcgcgg
    10921 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
    10981 cgatagccat ccacccgatg caaaaaatgg gtatgtgact gaaaatcaaa aacatcgcga
    11041 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
    11101 tgacgtttga tgcgcgaggt aacaacaaac gccgtcgcct tcatgccata ttgtttcaat
    11161 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
    11221 cgcgcaggga gattgatctt attcttcacg taaccttcca gctgcgccat gctcagtgtc
    11281 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
    11341 gtcgaagtat ggcgaaaacg ggtgttttct tcatcgcgca gaatatggtg ataggttagc
    11401 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggcgag tcgatcgcca
    11461 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
    11521 cgcaaattgt ccgccagtac cccaaatggc gcactgcccg cactcggcgc gttatagacc
    11581 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
    11641 ccgtcttcaa ctttttgtcg cccctgaact ggctcgagat gccctttatc gataaaccct
    11701 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
    11761 tgcccggccc gaatattccc cacggtcacc atcttgtcgc caatttgcgc ccagatcgca
    11821 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
    11881 agcatcagca ggagaataac agcttgtttg tacatgaaaa aataaccgag ggtgaaacaa
    11941 cgcttttatt gtagcaaaag cgctataaga atcgtgggtt aatactcata caaattatgt
    12001 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
    12061 cccagttcga caatacgctc gcgaaactct gaactggtca tggtttcacg gcaagccatc
    12121 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cacaacggga gtgtttatgg
    12181 cttaataatg aagccacgcg gtacggtggc gctccggcta aatcggttaa gaagatgaca
    12241 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
    12301 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
    12361 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
    12421 gccttcctcc ttagccgaaa atcagtctac cctgaccatt gttgataaag ggtgatagcc
    12481 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
    12541 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
    12601 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
    12661 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
    12721 tcagggagta aaacaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
    12781 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
    12841 gcaccgccgt gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
    12901 caaaaaacag tgaaattgcc gtcaccagaa tgccaacaaa cagaccacgc gccacgccac
    12961 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
    13021 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcattggtga
    13081 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
    13141 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
    13201 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
    13261 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
    13321 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
    13381 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgca gcacttcaac
    13441 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
    13501 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttagccagca gcgccttcat
    13561 cgaggtattt tccaccagct caccgtgttg aataatgccg atattgcggc acagcatttc
    13621 tgcttcttcc aggtagtgtg tggtgagaat gatggtggtg cctttgtcgt ttaaatcctt
    13681 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
    13741 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
    13801 gccagataac atacgcgcac gttcgttgcg ttttccccat agatcgagct gcttaagata
    13861 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
    13921 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
    13981 ctgacgttta gcgttcacga catccttctc gagatcgtaa ccaaatacgc tgacccgccc
    14041 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
    14101 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctatcc cacgaagcgc
    14161 ctgaacgccg cctggatagg tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
    14221 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
    14281 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
    14341 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
    14401 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
    14461 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
    14521 gttgctaacc tggtcattca cactgacctg aactgccttt ccgtggttca gtatgcagtg
    14581 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
    14641 gccgcagttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
    14701 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
    14761 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
    14821 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
    14881 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
    14941 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
    15001 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccgatat
    15061 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
    15121 ggatcgagaa accgataaat tctaccggga cgttcacttc acgacgggac ggtttatcca
    15181 gcagcgtaca aatcgccagc gacttcggtt cgcgcaggct taagatctca cgcactttcg
    15241 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
    15301 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
    15361 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
    15421 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
    15481 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
    15541 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
    15601 gcacgggcag gttttggcac cgcaacaaag cgcaagacac gcagtatacc agtaaaacta
    15661 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acgatcacac atgttaaaac
    15721 ctataattaa ttgctactaa aatattaatg aattgaaata gtgtctcttt atggcaatta
    15781 acaatacagg ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
    15841 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
    15901 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgtgccg
    15961 cgctttggct atacgccgcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
    16021 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
    16081 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
    16141 tggtggtggc actgctgatt agcggtagta tcctgacctg ggccggattt aacgatccgc
    16201 aggaaatcaa cggcacctta agcgccgatg ccacacctgc ggaagctatc tcacccgttg
    16261 ccgatcagga ctggcctgcc tatggtcgca atcaggaagg tcaacgcttt tcacctctga
    16321 aacaaattaa cgccgataac gtccataatc tgaaagaagc ctgggtgttc cgtactggcg
    16381 atgtgaagca gccgaacgat ccgggcgaaa tcaccaatga agtgacgccg attaaagtgg
    16441 gcgacaccct ttacctgtgt accgctcacc agcgcctgtt tgcgctcgat gccgccagcg
    16501 gcaaagagaa atggcattac gatcctgagc tgaaaactaa cgagtctttc cagcacgtaa
    16561 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
    16621 cggattgccc gcgtcgtatc attcttccgg tcaatgatgg tcgactgatt gcgattaacg
    16681 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
    16741 atatgccaga caccaaaccg ggtctgtatg aaccgacttc gccaccgatt atcaccgata
    16801 aaaccatcgt gatggcaggt tcagttaccg ataacttctc aacccgcgaa acgtctggcg
    16861 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat cccggcgcga
    16921 aagatccgaa cgcaatcccg tctgacgaac acacctttac ctttaactcg ccaaactcgt
    16981 gggcgccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    17041 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    17101 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    17161 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    17221 aagtgccagt tatttacgct ccggcgaaaa ccggcaacat ttttgtgctc gatcgtcgca
    17281 atggcgaact ggtggttccg gccccggaaa aaccggtgcc gcaaggtgct gcgaaaggcg
    17341 attacgttac tccgacccag ccgttctctg aactgagctt ccgtccgaag aaagatttga
    17401 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    17461 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggcacg ctggtcttcc
    17521 cgggtaacct gggaatgttt gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    17581 caattgccaa cccaatggca ctgccgtttg tttcgaaact gatcccacgc ggtccgggta
    17641 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    17701 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccgttt ggtctgccgt
    17761 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaaccaat gaagtggtgt
    17821 ggaagaaacg tattggtacg ccgcaagaca gtatgccgtt cccgatgcct gtaccggtgc
    17881 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    17941 ttatcgctgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    18001 tgtggcaggg ccgtttacca gcgggtggtc aggcgacgcc aatgacctat gaagtgaatg
    18061 gcaagcagta tgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    18121 actatattgt ggcttatgcg ctgccggatg atgtgaaata accataatgc ccggagagat
    18181 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    18241 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    18301 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    18361 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactggg tgccgtgaat
    18421 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    18481 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    18541 atggtggaaa tcgaacttcc cgccatggtt catatgattc atattgccgt gccccatatg
    18601 gcccatcatc tggctatgat ccatcccggc catcgcctga tcgccatatt tctccatcag
    18661 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    18721 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtctggca aagcaccgga
    18781 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggcgcaat
    18841 cgccatcccc atctggctga ccggtagcgt caccaggtca aagggtttgt tgtcgttaac
    18901 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    18961 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    19021 gaaattgagc gagcgggcat tacagccatt aagcaaacgc aggcgcagcc aaccacgcgg
    19081 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    19141 ggcggcggtc atcacatcca gttgataatc aatctgccca tcagcgttaa attttttatc
    19201 ctgaacaatc accggaacat catcgatgcc ccactgtttt ggcagcatta atttcaggat
    19261 ctcgtcatct tcaattacca ccagcccagc caaccccatc gccacctgtc gcccggtctt
    19321 gccatgttga tgtggatgga accagcaagt agcggcaggt tgatcaacgt tcaacgtcac
    19381 cgagcgcttg ccacctggca gaataattcc ctgcgggccg ccgtcgactt cacccggtac
    19441 ttccagcccg tgccagtgca acgtcgtctc ttccgtcagt tggttataga tatcaaccgt
    19501 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    19561 ggtagttgca gttttctcgc caaaggtaga ctgacctgcg ccaatagtta actgaatgcg
    19621 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    19681 gactgcgcgg ctccacagcg gcaaggctga agccacaccc agcgcgacgg aatattttaa
    19741 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    19801 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    19861 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    19921 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatggccgt ttgcgcaaac
    19981 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    20041 tttctgaaga acgattgtgg ttaccagaac ttacctaacg ggcaaattcg tcgcgcactg
    20101 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    20161 gccctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaag
    20221 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    20281 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    20341 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    20401 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    20461 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    20521 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    20581 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    20641 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    20701 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    20761 tacgacgatc cgcgctttaa gctggtgatc gacgatggcg tcaatttcgt taatcaaacc
    20821 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagt
    20881 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    20941 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    21001 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    21061 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    21121 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    21181 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    21241 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    21301 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    21361 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    21421 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    21481 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    21541 acagaacacc ccggcccact gccagaaacg gtcgttgccc atctcgataa aagtcatatt
    21601 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    21661 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    21721 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac ccgcgacatt
    21781 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    21841 gatatgaagg cgctgtatga catggtggat gtgaacgtct atcaggaaaa tatcttccat
    21901 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    21961 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    22021 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    22081 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    22141 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    22201 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcaccg tccatccaca
    22261 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    22321 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    22381 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    22441 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg cattgcgcca
    22501 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgatg gtttagaaat
    22561 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    22621 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    22681 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    22741 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccaatt
    22801 ctgcagaagc caggaagaca ttcgcgccag tccccagacg gttcgggaag ttacgggtag
    22861 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    22921 agccagggat ctcgatacgc gcaccgctct taccgaagac gctatagtag ccttcttcgg
    22981 tcaactgcgc ggcatccata cgggttggcg gtgccaccca caggcgcgtc ggcaactggc
    23041 ctttgtgcgc atccagcagt ttacccgcag cacggaagtg accgatgttg gtcatgcagg
    23101 aaccgataaa cacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcgt
    23161 ccgggtcgtt cggtgcacag aggattggct ctttaatatc cgccagatcg atgtcgatca
    23221 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    23281 ccatgccctg aatacgacgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    23341 acttcagcag gacgatgtta gagttcaggt attcgatgat cggttctttg ttcagcttga
    23401 tggtacaacc agcggcagaa cgctcggcgg acgcatcggt cagttcaaat gcctgctcaa
    23461 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    23521 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcgggatc gcgtgtacca
    23581 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    23641 gcatatccag cggcattacg cctgttgcgg ctgcaaatgc caccagacca gaacccgccg
    23701 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    23761 gcagcagcat acggttcagc caggagtgga ttacgccgtc acccggacgc agcgacacac
    23821 caccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    23881 gatacgccgc ggtatggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    23941 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgtgaa cctacagaag
    24001 tcattttcgg ttcgcagtac gcgcccggac gaataccttt cacaccgcag gcacgaccta
    24061 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    24121 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    24181 taatcagcgg gatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    24241 cgaaggtcgc cagcagttcg ccggtttcgt ggttacgcac ttcacctttg tacgggtaaa
    24301 cgtcaatcac gtcgcccatg ttcaggttag agacgtcgac ttcgattggc agtgcacccg
    24361 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    24421 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    24481 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    24541 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    24601 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgcgacc
    24661 acgcatccgg tgccggagaa aggtcatcgg tgttagtttc gccagtgact ttgaagacag
    24721 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    24781 actgcataac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    24841 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    24901 catcatccag cgcgtcgatc agcgaatgaa tgttgtaacc accctgcatg gtgcccagca
    24961 gttcgatggc tttttccgga gtcagcagag gggatttggc ttcgcctttc gcgatagcag
    25021 ccaggaagcc tgctttgaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    25081 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagtg
    25141 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    25201 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    25261 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    25321 aacgggcaga atagcaggat tttgacgatg tgttaatctg tttacaaaaa agcaacataa
    25381 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    25441 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    25501 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    25561 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    25621 aatttgcagt gccgggcttt gtgccgcctc taccgggcta tatataaaaa gccgcacagt
    25681 ggaagcgcct gtggaaccgc aatcgacaca acagactgcg cctgacatca ccgcagttac
    25741 gcttcctgca acggtttccg caccgcccgt aacgcccgcc gtcgtcaaat ccgcattcag
    25801 cactgcacaa atcgatcaat gggttgcgcc tgtcgcgctg taccccgatt ctctgctttc
    25861 acaagtgtta atggcatcaa cctatccggc aaacgttgct caagcagtgc aatggtcgca
    25921 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    25981 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    26041 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    26101 ggtacagcga ttgcggcaac tggcgcaaca aaccggttcg ctgaagtcat caaccgaaca
    26161 aaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    26221 cataccatcc aataccgttt taactgccag ccccgtcatt acagagcctg caacaaccgt
    26281 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    26341 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    26401 agaaccgttt attgacagct ttgtgcgcgg attcggctat agcatgggtg ttgctaccac
    26461 gtacgcacta ttcagcagca tcgactggga tgacgacgat catgaccatc atcatcatga
    26521 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac ggtaatggct ggcaacacaa
    26581 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    26641 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    26701 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gcgccacgca
    26761 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    26821 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    26881 taatgaagct gaaaactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    26941 actgacccag caacaaaagg acgccgctcg tcagcgttat cagtcggcct cgcctgagca
    27001 gcgccaggca gtccgcgaga aaatgcagac taacccacag atccagcagc gaagagacgc
    27061 agcgcgtgag cgtattcagt cagcctcgcc tgagcagcgc caggtgttta aggaaaaagt
    27121 acagcagcgc ccactgaacc aacagcaacg tgataacgcc cgccagcgtg ttcaatcagc
    27181 atcacctgaa caacgtcagg tttttcggga aaaagttcag gagagccgcc cacaacgtct
    27241 aaacgacagt aaccatactg tcaggctgaa taacgagcaa cggtcagcag tacgcgaacg
    27301 tctctctgag cgcggagcaa ggcgacagga aaggtaaatt gcagacgtaa aaaaagcggc
    27361 gtggttagcc gcttttttaa ttgccggatg ttccggcaaa cgaacaatta cttcttcttc
    27421 gctttcgggt tcggcaggtc ggtaatgcta ccttcgaaca cttctgccgc caggcccaca
    27481 gactcgtgca gagtcgggtg cgcgtggatg gtcagtgcga tgtcttcagc atcacaaccc
    27541 atttcgattg ccaggccgat ttcacccagc agctcgccgc cgttggtacc gacaatcgca
    27601 ccaccgatca cacggtgaga ttctttgtcg aaaatcagct tggtcatacc gtctgcgcag
    27661 tcggaagcga tagcacgacc agaagcagcc cacgggaagg tggcggtttc atagctgatg
    27721 cctttctctt tcgcttcttt ctcagtcaga cctacccatg caacttctgg ttcggtatag
    27781 gcgatggacg ggataacttt cggatcgaag tagtgtttct taccggcgat aacttcagcg
    27841 gcaacgtgac cttcgtgaac acctttgtgt gccagcatcg gctgaccgac gatatcgccg
    27901 atagcaaaga tgtgcggtac gttggtacgc agctgtttgt caacgcggat gaaaccacgg
    27961 tcgtccactt ccacgccagc tttgcctgcg tcgaggtttt taccgttcgg cacacgacca
    28021 atcgctacca gcacggcgtc gtaacgctgc ggttcagcgg gtgctttttt gccttccatc
    28081 gtcacataaa taccgtcttc tttcgcttca acggcggtaa ctttggtttc cagcatcagg
    28141 ttgaatttct tgctgatacg cttggtgaag actttaacga tgtctttgtc agctgccggg
    28201 ataacctggt cgaacatttc aaccacgtca atctgtgaac ccagcgcgtg gtatacggtg
    28261 cccatttcca gaccgatgat accgccaccc attaccagca ggcgttctgg tacttctttc
    28321 agttccagcg cgtcagtgga gtcccagata cgcggatctt catgcggaat aaacggcagt
    28381 tggatcgggc gagaacccgc tgcaatgatc gcattgtcga agttgatcac ggttttgccg
    28441 ttctcacctt caacttccag ggtgttagcc ccggtgaatt tacccagacc gttgaccact
    28501 ttgactttgc ggcctttcgc cataccagcc agaccaccgg tcagctgatt gatcactttc
    28561 tctttccagg tacgaatctt gtcaatatcg gttttcggtt cgccgaagac gataccgtgt
    28621 tcagccagcg ctttggcttc ttcgataact tttgctacgt gcagcagtgc tttagaaggg
    28681 atacagccga cgttcaggca aacaccgcca agggtgttgt aacgttctac gattacggtt
    28741 tccagaccta aatcagcgca acggaaggca gcggagtaac ctgcggggcc tgccccaagt
    28801 accacgacct gagttttgat ttcagtactc atcatgacct ctatatattt atctccggcg
    28861 gtcatacccg tcgtctttca ggccgcagcg gcgttagctt cacgccctca ccccagtcac
    28921 ttacttatgt aagctcctgg ggattctggc gcttgctacc ttgctgcacc cagaaatcca
    28981 tagggtatgt gaagtaatac cctaaccacc accgggtcgt tctatccgtc ggtattttac
    29041 aaaattgtta acaattttta aacaacaaac ggcaaccgat ttgtctattc gctaataacc
    29101 tcaatacatt catgagatta ccagaaaaaa gccggccgtt gggccggctc ttttacttac
    29161 atcaccagac ggcgaatgtc agacagcgtg ttgttaatga tggtaatgaa acgggcacca
    29221 tcagcaccgt cgatcacgcg gtggtcgaag gagagagaaa tcggcagcat cagacgcggc
    29281 acgaactctt taccattcca caccggctcc atcgcggact tggaaacgcc gaggatagcc
    29341 acttccggcg cgttcacaat cggcgcgaag tgggtagtac ccaggccgcc gatgctggag
    29401 atggtgaagc aaccgccctg catttcgccc gcagtcagct taccgtcacg cgctttctta
    29461 gaaatagtca tcagctcgcg agacagctcg atgatgcctt tcttgttgac gtctttgaat
    29521 accggaacaa ccagaccgtt cggggtatcc accgccacac cgatgttgat gtatttcttc
    29581 agggtcagac gctgaccgtc ttccgacaac gaactgttga agcgaggcat ctgctcaaga
    29641 gctgcagcaa cggctttcat gatgaagaca accggggtga tcttcacatc cagcttacgt
    29701 ttcgccgctt cttcgttctg ctgtttacgg aacgcttcca gctcggtgat atcggttttg
    29761 tcgaagtgag taacatgcgg gatcatcacc cagttacggc tcaggttcgc accagaaatt
    29821 ttctggatac ggcccagttc cacttcttcg atttcaccaa acttgctgaa gtccaccttc
    29881 ggccacggca gcatgcctgg gataccgccg ccagtcgccg ccggagctgc ttctgcacgt
    29941 ttgatagctt ctttcacgta agcctgaacg tcttcgcgca ggatacgacc tttacggcca
    30001 gtgcccttca ctttcgccag gttaacgcca aactcgcgtg ccagacggcg gatcagcgga
    30061 gtcgcgtgaa cgtaagcgtc gttttctgca aattcagatt tgccttccgc tttcgcagcc
    30121 ggtgctgctg ccggagcttc agcttttgct gccggagccg gcgctgccgc ttcctgtttc
    30181 gcaggagccg ccgcaggcgc tgcgccttca acttcgaaga tcataatcag cgagccagtt
    30241 ttcactttat cgccaacgtt gactttcagt tccttcacga cgcctgcaaa cggagccgga
    30301 acttccatag aagctttgtc gccttctacg gtaatcagtg actgttcagc ggcaactttg
    30361 tcgcccactt tcaccatcac ttcggtcact tcaacttcgt caccgccgat atccggaacg
    30421 ttaacttctt tcacgccagc cgctggtgca ggggccgctg ccggagccgc ttcctgttta
    30481 gccgccggag ctgccgcgcc tgcttcaccc gcgacttcga agaccataat cagcgagccg
    30541 gtagacactt tgtcacccac gttcactttg atctctttca cggtgccagc aaacggagcc
    30601 ggaacttcca tagacgcttt atcgccttct acggtgatca gcgactgttc agcttcaact
    30661 ttatcgccaa ctttcaccag gatttcggtc acttcaactt cgtcgctgcc gatatccgga
    30721 acgttaacgt cttttgccgc tgcagccgct ggtgctgctg ccggagctgc ttctttcttc
    30781 tcttctgcct gagcaggtgc agcgtctgct gcaccgtcgg cggaatcgaa aatcataatc
    30841 agtgcgccgg tctgggtttt atcgccaaca gagactttga tctctttaac gatacccgcc
    30901 tgcggagacg gaacttccat agaggctttg tcgccttcta cggtgatcag cgactgttcg
    30961 gcttcaactt tgtcgcccac tttgaccagg atctcggtga tttcaacttc atcagccccg
    31021 atgtccggta ctttgatttc gatagccatt attcttttac ctcttacgcc agacgcgggt
    31081 taactttatc tgcatcgatg ttgaatttgg cgattgcgtc agcaaccact ttcttatcga
    31141 tttcgccacg tttagccagt tcgcccagcg ccgctacaac cacgtaggaa gcatcaactt
    31201 cgaagtggtg acgcaggttc tcacggctgt cggaacgacc gaagccatca gtacccagta
    31261 cgcggtagtc gtcagccggt acgtaagtac ggacctgctc agcgaacagt ttcatatagt
    31321 cggtagatgc cactgccgga gcgtcgttca tcacctgagc gatatacgga acgcgcggag
    31381 tttccagcgg gtgcagcatg ttccagcgtt cacaatcctg accatctcgc gccagttcgg
    31441 tgaaggaggt cacgctataa acgtcagaac ctacgccgta atctttcgcc aggatctctg
    31501 ccgcttcacg gacgtgacgc aggatagaac cggagcccag cagctgaact ttacctttgc
    31561 taccttcaat ggtttcgagt ttgtagatac ctttacggat accttcctca gcaccttccg
    31621 gcattgccgg catgtggtag ttttcgttca gcgtggtgat gtagtagtaa acgttctctt
    31681 gtttttcacc gtacatacgc tccagaccgt catgcataat gacagcaact tcgtaagcgt
    31741 aagccgggtc gtaagagata cagttcggaa tagtcagcga ctgaatgtgg ctgtgaccat
    31801 cttcgtgctg cagaccttcg ccgttcaggg tggtacgacc ggaagtaccg ccgatcagga
    31861 agccacgcgc ttgctggtcg ccagccgccc agcacagatc gccaatacgc tggaagccga
    31921 acatcgagta atagatgtag aacgggatca tcggcagatt gttggtgctg taagaggtcg
    31981 ccgctgccag ccaggaacaa cctgcgccca gctcgttgat cccttcctgc agaatctgac
    32041 ctttctcgtc ttctttatag taagcaacct gctcgcggtc ctgcggggtg tactgctgac
    32101 cgttcgggct gtaaatacca atctgacgga acagaccttc cataccgaaa gtacgcgctt
    32161 cgtcggcgat gatcggtacc agacgatctt tgatcgactt gttcttcagc attacgttca
    32221 gggcacgaac gaaagcgata gtggtagaga tctctttgct ctgctcttcc agcagcgcgc
    32281 cgaagtcttg caggctcggc agctcaagct tctcggtgaa gttcggctga cggcttggca
    32341 gataaccgtg cagtttctga cgctgagcgt gcagataggt atgctcttca gaaccttccg
    32401 ggaaggtgat gtacggcagt ttttcgatat ctgcatcaga caccggcaca ttgaaacggt
    32461 cgcggatgtg acgcacgccg tccatgttca ttttcttaac ctggtgcgcg atgtttttac
    32521 cttcagccgc gtcgcccatg ccgtaacctt taatggtatg agcaaggatt actgtcgctt
    32581 tgcctttggt ttcctgcgct ttcttgaatg cagcgtagat tttcttcgga tcgtgaccac
    32641 cacggttcag tgcccagatc tgctcgtcag tccagtctgc aaccagtgct gcggtttcag
    32701 gatatttacc gaagaagtgt tcacgaacgt acgcaccatc tttcgatttg aaggtctggt
    32761 agtcgccgtc aacggtttcg ttcatcagct ggatcagttt accgctggta tctttacgca
    32821 gcagttcatc ccaacggcta ccccacatca ctttgatcac gttccagcca gcaccttcga
    32881 agatgccttc cagttcgttg atgatcttgc cgttaccggt gaccgggccg tcaagacgct
    32941 gcaggttaca gttgataacg aagactaggt tatccagttt ttcacgggta gcgatggtga
    33001 tcgcaccttt ggattccggt tcgtccattt caccgtcgcc gaggaacgcg taaacggtct
    33061 gtttagaggt atctttcagg ccacggtgtt ccagatattt caggaattta gcctggtaaa
    33121 tagcaccaat cggccccaga cccatagata cggtcgggaa ctgccagaat tccggcatca
    33181 gtttcgggtg cggataggaa gagaggccat tgccgtgaac ttcctgacgg aagttatcca
    33241 gctgctcctg agtcagacga ccttctagga aagcacgagc gtacacgccc ggggagatgt
    33301 ggccctggaa gtaaaccagg tcgccgccat cctgctcgtt gcgtgcacgg aagaagtggt
    33361 taaagcacac atcataaatg gttgcggaag actggaagga cgccatatgg ccgcccagtt
    33421 cgaggtcttt tttcgacgca cgcagcaccg tcatgatggc gttccagcgg atagctgaac
    33481 gaatacggcg ttccagttcc agattacccg gatactccgg ttgttcttca acggggatgg
    33541 tgttgatgta gttgctgata cctgtgcctg cggctacgtt tacaccgcct ttgcgggctt
    33601 cagcaagcag ttggtcgatc agatactgag cacgctcaac accttcttca cggatgaccg
    33661 attcgatcgc ctggagccag tcgcgagttt cgatcggatc cacgtcattt gggaaacgtt
    33721 ctgacatggg ttattcctta tctatctaat aacgttgagt tttctggaac ctgtcccatt
    33781 gaactctcgc cggaaagctc aataagacag gttctacgtt tagttgccgc gcttttatat
    33841 gcgcttgatt tacaacatct tctggataat ttttaccaga aaaatcacta attctttcgt
    33901 tgctccagac gacgcagaga acgctcacgg cggctctctt cacgacttct gtcgagcaaa
    33961 atttcttcga taaaggccag atggcgatgc gatgcttcgc gcgcttcttc cggcttaccg
    34021 gccataatcg cttcaaatat gcgggtgcgg tgactactca ccagcggcag catctcgcga
    34081 cgcgaataga gcaattcgaa gttctggcgg acattctggg ccaacatcgg ctccatacac
    34141 cttagcagat gaagcagaac cacattgtgg gccgcttcag tgacagcaat ctgatactgg
    34201 agtacggcgt ttgattccgc gtccagatcg ccagactgct gcgccagctc tatggcgtgg
    34261 tggagttcac ggatgcgttc cttgtcttca tcggtactac gcagcgcggc gtaataagcg
    34321 gcgatacctt ccagggcgtg tcgtgtttcg agcaagtcat actgtgactc aggatggtcg
    34381 gagagcagct ccaccagcgg atcgctgaag ctttgccata ggctgctctg gacaaaagtg
    34441 ccgccaccct ggcgacgaag caacaagccc ttcgcttcga gacgttgaat cgcctcacgc
    34501 aaggagggac gggagacgtc aaactgtttt gccagttcgc gttccggtgg gagtttttcg
    34561 cccgggcgga gagtgccttc gaggatcaaa aactccagtt gctgctcaat cacatcggag
    34621 agttttggtt ggcggatttt gctgtaggcc atgagttcct gtcttaagcc acttgccgaa
    34681 gtcaattggt cttaccaatt tcatgtctgt gacgctaaag taacaaagta ttcaccttat
    34741 gtccatacag gttttgattg aaatcatgaa actgtgcaca ttttaacaac ttgacatata
    34801 taacgtttca aagttgtaac tatgcacaaa tgtagacttt acgtaggaaa ggagtttttt
    34861 aacgattatt aaactataaa aatccgaatt gaaccgattc acttaccaat tttgtgattt
    34921 ttaattcaat taaaacgaat ttaaattcat tctacatatt gagaggggtt gaggctgagc
    34981 tttacaaacg gtttcttttt aagcaactca tcttcaacca tgcataaagc gggtgcattc
    35041 gctgcctcat accattattc ttgatctgac ggaagtcttt ttgtaacaat tcaaacttct
    35101 ttgatgtaaa caaattaata caacaaacgg aattgcaaac ttacacacgc atcactgcgt
    35161 agatcaaaaa aacaaccacc gcacgaggtt tcatgatgga aggtcaacag cacggcgagc
    35221 agctaaagcg cggccttaaa aaccgccata ttcagcttat cgcgctgggt ggcgcgatag
    35281 ggaccgggtt attcctgggt agcgcctccg taatacagtc cgcagggcca gggattatcc
    35341 tgggttacgc cattgctggt tttatcgcct ttctgatcat gcgtcagctg ggtgaaatgg
    35401 tggtcgaaga acctgtcgca ggctccttta gccactttgc ttataaatac tggggcagtt
    35461 ttgccggttt cgcctctggc tggaactact gggtactgta cgttttagtt gccatggctg
    35521 agctgactgc cgtgggtaaa tacattcagt tctggtatcc ggaaatcccc acctgggttt
    35581 ctgccgccgt attctttgtg gtgattaacg ccatcaacct gaccaacgtt aaagtgtttg
    35641 gcgagatgga gttctggttt gccattatca aagttatcgc ggtggtagcg atgatcatct
    35701 tcggcggctg gctgctattc agtggcaacg gcggcccgca ggcgaccgtt agcaacctgt
    35761 gggatcaggg tggtttcctg ccgcacggct tcaccgggct ggtgatgatg atggcgatga
    35821 tcatgttctc gttcggtggt ctggaactgg tggggatcac cgcagcagaa gctgataacc
    35881 cggagcaaag tataccgaaa gcaactaacc aggttatcta ccgcatcctg attttctata
    35941 ttggttcgtt agccgttctg ctctcactga tgccgtggac ccgcgttacc gccgatacca
    36001 gtccgtttgt gctgatcttc cacgagttag gcgatacctt tgtggcgaat gcgctgaaca
    36061 tcgtggtact gactgcggcg ctctccgtgt acaacagctg cgtatattgc aacagccgta
    36121 tgctgtttgg tctggcacaa cagggtaatg cgccaaaagc gctggcgtct gtcgataaac
    36181 gtggtgtacc agtaaatacc attctggtgt ctgcactggt aacggcgttg tgcgtactga
    36241 ttaactacct tgccccagag tccgctttcg gactgttaat ggcgctggtg gtatctgcac
    36301 tggtaatcaa ctgggcgatg attagcctgg cgcatatgaa attccgtcgc gccaagcagg
    36361 aacaaggcgt ggtaactcgc ttccctgctc tgctttatcc gctgggtaac tggatctgcc
    36421 tgctgtttat ggcggcggta ctggtgatta tgctgatgac cccaggaatg gcgatttcgg
    36481 tatacctgat cccggtatgg ctgatcgtgt taggtatcgg ctatctgttt aaagagaaaa
    36541 ccgccaaagc cgtaaaagcg cattaatctc tctacgccct cacccgtaca gggtgagggc
    36601 aataatcttt acaccaacgc cccgtaaatc gtcagtagtg caatcaccac cacgaccacg
    36661 aacgaggttt tcttcgccat tgaaaccgct gccctcggcg tctccacctt atcgacatgc
    36721 ggttcacgcg ccagagagaa ctgcgccaga cgcgttaaca cctgatactg cgaagtatgg
    36781 aaatcaccca gcgaagcaaa ccaggccggt aacgctttct caccatgacc gatcaaggca
    36841 tataccacac ccgcaagacg aaccggcacc caatccagta catgaagcac ggcatcaatg
    36901 ccggactgta aacgatgatg cggcgtctga tatcgcgcca gccagtattg ccatgcacgc
    36961 aaaaacgcat accccatcag cgtaacgggt ccccaggttc cccccacaat cagccagaac
    37021 agcggtgcaa gataaaaacg aaagttaatc cacagcaatg cattttgcag ctcacgcaaa
    37081 tactcacgtt cgtcgcagcc tgccgggacg ccgtgaatca tggtgagttc gccagccatc
    37141 gtggcacggg catggctatc attacgtgaa gcagctgtca gataagcatg ataatgaaga
    37201 cgaactttac ctgcgccaat acacagcaaa ccaatcagca gccacaccag tagcgtggga
    37261 acgttgaaca atactccctg caatgcgcgt aacagtaaaa aagtcacgcc catcgcaata
    37321 atggtcatgc ctaacgtgcg cccgagagaa aaatgtttca cccgccgaaa gaacgcttca
    37381 agacgatgat caagctgcca gtgctcgccc aacttaaaca ggcgctcgaa aattaacacc
    37441 agtaaggttg taaatagcgt catgttgtct ccttgctgac cagcacacga aaccgtgccc
    37501 aatcaaatgc aggaccggga tcggttttcc gatccggcgc aatatcacaa tggcccgtca
    37561 tgtttttagc gatatccgga tagcaatcaa tcagtgcccg cgtaaccgcc gcaagctgtt
    37621 gatactgcgc atcggtatac gccagcgtat cggtgccttc aagctcaatc ccaatagaaa
    37681 aatcattgca gcgttcgcgc ccctgatact gagagactcc cgcatgccat gcacgtttat
    37741 cgaaaggaac atactggact atttcaccat cacggcgaat caaacagtga gcggagacgc
    37801 gcaaatgggc gatctcagca aagaaaggat gtgcctgcgg atcaatagtt ccagtgaata
    37861 atgcgtcgat ccacggaccg ccaaactcgc ctggcggcag gctaatattg tgcaccacca
    37921 gcagggtggg tgtttcgtca tccgggcggc aatcgtaatg tggtgaggga acgcggcgcg
    37981 cgccaaccag ccacccctgt tctaacaaca tgcaggatct ccttatatgt ggtgctaata
    38041 cccggttcag agtagcatgt ttctacctta tgattcgtta gctatctgga gttttaacat
    38101 gccgcctcgc cgctataacc ctgacacccg acgtgacgag ctgctggaac gcattaatct
    38161 cgatatcccc ggcgcggtag cccaggcgct gcgggaagat ttaggcggaa cagtcgatgc
    38221 caacaatgat attacggcaa aacttttacc ggaaaattct cgctctcatg ccacggtgat
    38281 cacccgcgag aatggcgtct tttgcggcaa acgctgggtt gaagaggtgt ttattcaact
    38341 ggcaggcgac gatgtcacca taatctggca tgtggatgac ggcgatgtca tcaatgccaa
    38401 tcaatccttg ttcgaacttg aaggcccatc ccgcgtgctg ttaacgggcg aacgcactgc
    38461 gcttaatttt gtgcaaaccc tttcaggagt tgccagtaag gtacgccact atgtcgaatt
    38521 gctggaaggc accaacacgc agttgttgga tacgcgcaaa accttacccg gcctgcgttc
    38581 agctctgaaa tacgcggtac tttgcggcgg cggagcgaat caccgtctgg ggctttctga
    38641 tgccttcctg atcaaagaaa accatattat tgcctccggc tcagtgcgcc aggcggtcga
    38701 aaaagcgtcc tggctgcacc cggatgcgcc agtagaagtc gaagtagaga atctggaaga
    38761 acttgatgaa gccctgaaag caggagccga tatcatcatg ctggataact tcgaaacaga
    38821 acagatgcgc gaagccgtca aacgcaccaa cggcaaggcg ctactggaag tgtctggcaa
    38881 cgtcactgac aaaacactgc gtgaatttgc cgaaacgggc gtggacttta tctccgtcgg
    38941 tgcgctaact aaacacgtac aagcactcga cctttcaatg cgttttcgct aatagttgac
    39001 agatttatcc gctccatcgc gggcggataa tctccccttc cccaactttc ttcgtaacgc
    39061 ctcgcaaatt tcttgtcttt cgctgcaacg aaataaaaaa cctcggaatc tgctttcccg
    39121 tttcgttttt tgcccctcgc ctgtgctgat ggcgtttgcc aaagtagcgc caactaaatc
    39181 aaggagcgaa acagatggac aagcaacgcg gttttacact tatcgaactg atggtggtta
    39241 ttggcatcat tgccatttta agcgccattg gtattcccgc ttatcaaaac tacctgcgca
    39301 aagccgcact caccgacatg ctacaaacct ttgtgcctta ccgtaccgcc gtagagttgt
    39361 gcgcgctgga acatggtgga ttagatacct gcgacggtgg cagcaatggc attccctcgc
    39421 ctaccaccac ccgctatgtt tcagccatga gtgtggcaaa gggcgtggtg tcgctgaccg
    39481 ggcaagaaag tctcaatggg ctaagcgtcg tcatgacacc gggttgggat aacgcaaacg
    39541 gcgtcaccgg ctggacgcgc aactgcaata ttcaaagtga cagcgcattg catcaagcct
    39601 gcgaagatgt cttccgcttt gatgacgcca actaaggagc ggcaatgaat attccacagc
    39661 tcactgccct gtgtctgcgt tatcatggag tcttgctgga tgccagcgaa gaggtggttc
    39721 atgttgcggt agtcgatgca ccttcgcatg agctactgga cgcattgcat ttcgctacca
    39781 ccaaacgtat tgagatcacc tgctggacgc gccaacaaat ggaaggtcac gccagtcgca
    39841 cacaacagac attgcccgta gctgttcagg agaagcatca gcccaaagca gagttgctaa
    39901 ctcgaacgtt acaatctgcg ctggaacaac gcgcgtctga tattcatatc gaaccagcgg
    39961 acaatgccta ccgcatccgc ttgcgtatcg acggcgtatt gcatccttta ccggatgttt
    40021 caccggatgc cggagtcgca ttaaccgcca gattaaaagt gctgggaaac ctggatattg
    40081 cggaacatcg cctgccgcag gacgggcaat tcactgtcga actggcagga aacgccgtct
    40141 catttcgtat tgcgacctta ccatgtcggg gtggtgaaaa ggtggtatta aggttgttac
    40201 agcaggtgag ccaggcactg gatgtcaaca cgcttggaat gcagccgtta caactggcgg
    40261 actttgctca tgccttgcaa caaccacagg gactggtgct ggtaactggc cctacaggca
    40321 gcggcaaaac ggtcacgctt tatagtgccc tgcaaacgct gaataccgct gacattaata
    40381 tttgtagcgt cgaagatccg gttgagatcc ccatagccgg actaaaccag acgcaaatcc
    40441 atccgcgtgc cggactcacc tttcagggcg tgttgcgtgc gttattgcgc caggatcctg
    40501 acgtcatcat gatcggagag atccgcgatg gcgaaacagc agagatcgct attaaagcgg
    40561 cgcaaactgg tcacctggtg ttgtctaccc tacacactaa ttccacctgc gaaacgctgg
    40621 tacgtttaca gcaaatgggg gtcgcccgct ggatgctatc atcggcgctt acgctggtaa
    40681 tagcccagcg tctggtacgc aaactttgcc cacattgtcg ccggcagcaa ggggagccca
    40741 tccacattcc agacaatgta tggccatcgc cgctgcccca ctggcaggca cccggttgtg
    40801 tacattgcta ccacggtttt tatggtcgta cggccttatt tgaagttctg cccataacgc
    40861 cggtcattcg tcagcttatt tccgctaata ccgacgttga atcgctggaa acgcacgcac
    40921 gacaggcggg tatgcgtacg ctttttgaaa acggctgcct ggccgtggag caaggcttaa
    40981 ccacctttga agagttaatc cgcgtactgg ggatgccgca tggcgagtaa gcaactctgg
    41041 cgctggcatg gcataaccgg cgacggcaat gcgcaagatg ggatgctatg ggcagagagc
    41101 cgtgctttgc tgctcatggc actacagcaa cagatggtta ccccacttag cctgaagcga
    41161 atcgccatca attctgcgca gtggcgagga gataaaagcg cggaagtcat tcatcaactg
    41221 gcgacgctac tcaaagccgg gttaacgctt tctgaagggc tggcactgct ggcggaacag
    41281 catcccagta agcaatggca agcgttgctg caatcgctgg cgcacgatct cgaacagggc
    41341 attgcttttt ccaatgcctt attaccctgg tcagaggtat ttccgccact ctatcaggcg
    41401 atgatccgca cgggtgaact gaccggtaag ctggatgaat gctgctttga actggcgcgt
    41461 cagcaaaaag cccagcgtca gttgaccgac aaagtgaaat cagcgttacg ttatcccatc
    41521 atcattttag cgatggcaat catggtggtt gtggcaatgc tgcattttgt tctgccggag
    41581 tttgccgcta tctataagac cttcaacacc ccactaccgg cactaacgca ggggatcatg
    41641 acgctggcag actttagtgg cgaatggagc tggctgctgg tgttgttcgg ctttctgctg
    41701 gcgatagcca ataagttgct gatgcgccga ccgacctggc ttatagtgcg gcagaaattg
    41761 ctgttacgca tcccgattat gggttcactg atgcggggac aaaaactcac gcagattttt
    41821 acgattctgg cgctgacaca aagtgcaggc attacttttt tgcagggcgt agagagcgtc
    41881 agagaaacaa tgcgctgccc gtactgggtg caacttctga cacaaatcca gcacgatatc
    41941 agtaacggtc atcccatctg gctggcgcta aaaaataccg gggagtttag cccgctctgt
    42001 ttgcaattag tgagaacagg agaggcatcc ggctcgctgg acctcatgtt agacaacctc
    42061 gcccatcatc atcgggataa cacaatggcg ctggcggata acctcgcagc cttactggaa
    42121 ccggcgttgt tgatcataac gggaggaatt atcggtacgc tggtggtggc gatgtatctg
    42181 ccaattttcc atttaggcga tgcgatgagt gggatgggat aacgctggcg tgttgctcca
    42241 caccagcgtt ggaagattac aggttgttga agatgcggtt ttcttgttcc tgcacacgga
    42301 taaatgtggt gcgcttggtc agttctttca ggcgtgaagc cccaacgtat gtacaagctg
    42361 aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac cgggcctcgc agcggcagct
    42421 taacggtttt accttctgct gcgcgatatt ccgcaacgcc gccaacgtga cgtttcatcg
    42481 cagactcgga gctcatgccg tagaacagca taaatttctc gccgttctcc tcaacgatgc
    42541 gaccgccgct ctcttcgtgg cccgccagca tgccgccaag catgacgaaa tcggcaccgc
    42601 cgccaaaggc ttttgcgaca tcgcccggcg tggtgcagcc accgtcgctg acgatcattc
    42661 cgcccagacc gtgcgccgca tcggcacatt cgattaccgc agaaagctgc ggataaccga
    42721 cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc aatgccaact ttaacgatat
    42781 cagcaccgga gaggataagc tcctcacaca tttcaccagt cactacgttg ccagcacaaa
    42841 tggttttggt cggccacgct tcacgcgctt tcgcaacgaa ctgcacgaag tgttcggaat
    42901 aaccattcgc cacatcaata caaacgaagt ttaatgccgg gttcaggtcg agaatctgtt
    42961 tagttttttc gaaatccgca tcagacgtac cggtagaaac catcacatgt ttcagcacat
    43021 cagcggaaga attgttgata aacgcttgcc actcttcgac agaatagtgt ttatgcacag
    43081 cagtcaaaat atcaaaagaa gccagcgcag aggccataga aaatgtacct acggtgtcca
    43141 tatttgcggc gataattggc acgccggacc agctctgacc tgaatgtttg aaggtgaatt
    43201 gacgttccag ttcaacatcg gaacggcttt taagagtgga gcgtttaggg cggatgagaa
    43261 cgtctttaaa acctaacttc agatcttctt caatacgcat gtgcggattc ctggggttaa
    43321 tggctataag ggtaagagcg caactccagt gacgctatca tacgcactaa tcaatgcggc
    43381 gcaagactgc gaaattgtct ttttttacgc tacaatccca taaatttacc tggtaaatag
    43441 tgagcgaacg tttcatcaat ttcgctttcg tgcactcatt tttaacgttt tgataatcat
    43501 gatgaattcc cgggaatata aattatgagg tatatagttg ccttaacggg aggcattggc
    43561 agtggcaaga gtaccgttgc caatgcgttt gctgatctcg gaattaacgt cattgatgcc
    43621 gatattattg cgcgtcaggt ggttgaacca ggtgcacctg cgctacatgc cattgctgat
    43681 cactttggcg ctaacatgat tgctgctgat ggaacattgc agcgccgggc cttgcgcgag
    43741 cggatcttcg ccaacccgga agagaaaaac tggcttaacg ccctgctgca tccgctgatt
    43801 cagcaagaga cgcaacacca gatccagcaa gctacttccc cctatgtact gtgggttgtg
    43861 ccattgctgg tagaaaactc actgtataaa aaagcgaatc gagtgctggt ggtggatgtc
    43921 agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg atgtaactcg cgagcatgtc
    43981 gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc ttgccgtggc agatgacgtc
    44041 attgataata acggcgcacc ggatgctatc gcatcggatg ttgcccgcct gcacgcacac
    44101 tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac cgtaatgcag acccaggtcc
    44161 tttttgaaca tccactaaat gaaaaaatgc gtacatggct gcgcattgag tttttgattc
    44221 agcaactcac cgttaattta cccatcgttg accacgctgg cgcgctgcat ttcttccgta
    44281 atgtcagtga attactggat gttttcgagc gcggcgaagt ccgcactgag ctgttgaaag
    44341 aacttgaccg gcagcaacgt aaactccaga cctggattgg cgtgcctggc gtggaccaga
    44401 gccgtattga agcattaatt cagcagttaa aagcggcggg gagcgtatta atttccgcgc
    44461 cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc tctggtgcgt cagcgactga
    44521 gcatcccagg cggctgttgc agctttgatt tacctacatt gcacatttgg ctgcatctac
    44581 cccaggcgca gcgcgacagc caggtagaaa cctggattgc cagcctgaac ccgctcaccc
    44641 aggcacttac catggtgctg gatttaattc gccagtcggc tcccttccgt aaacaaacca
    44701 gcctgaatgg tttttatcag gataacggtg gcgatgccga cttgctgcgc ctgaatctgt
    44761 cgctcgattc acagctttat ccgcaaattt ccggacataa gagccgtttt gccattcgtt
    44821 ttatgccgct ggacactgaa aacggacagg taccggaacg tctggatttc gaactggcct
    44881 gttgctaagg agtgaaaatg tcagaaacta ttacggtgaa ttgcccaacc tgcgggaaaa
    44941 cggtggtgtg gggtgaaatc agcccgtttc ggccattttg ctccaaacgt tgtcagctga
    45001 tcgacctcgg agaatgggct gctgaagaaa aacgaatccc cagcagtggc gatctttccg
    45061 aaagcgatga ctggagcgaa gagccaaagc agtgacattt gtctgatgcc
//
LOCUS       DAZGJW010000020        43234 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZGJW010000020 DAZGJW010000000
VERSION     DAZGJW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.020"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHKIK_001185"
     CDS             complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHKIK_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO9082398.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHKIK_001186"
     CDS             complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHKIK_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO9082399.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHKIK_001187"
     CDS             complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHKIK_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO9082400.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLSVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(1087..1581)
                     /locus_tag="ACHKIK_001188"
     CDS             complement(1087..1581)
                     /locus_tag="ACHKIK_001188"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO9082401.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHKIK_001189"
     CDS             complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHKIK_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO9082402.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHKIK_001190"
     CDS             complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHKIK_001190"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO9082403.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHKIK_001191"
     CDS             complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHKIK_001191"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO9082404.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHKIK_001192"
     CDS             complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHKIK_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO9082405.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHKIK_001193"
     CDS             complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHKIK_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO9082406.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHKIK_001194"
     CDS             complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHKIK_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO9082407.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            8195..9235
                     /gene="gpr"
                     /locus_tag="ACHKIK_001195"
     CDS             8195..9235
                     /gene="gpr"
                     /locus_tag="ACHKIK_001195"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO9082408.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(9275..9769)
                     /locus_tag="ACHKIK_001196"
     CDS             complement(9275..9769)
                     /locus_tag="ACHKIK_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO9082409.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIINVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9960..10844
                     /gene="yghA"
                     /locus_tag="ACHKIK_001197"
     CDS             9960..10844
                     /gene="yghA"
                     /locus_tag="ACHKIK_001197"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO9082410.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHKIK_001198"
     CDS             complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHKIK_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO9082411.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHKIK_001199"
     CDS             complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHKIK_001199"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO9082412.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12534..13721
                     /gene="metC"
                     /locus_tag="ACHKIK_001200"
     CDS             12534..13721
                     /gene="metC"
                     /locus_tag="ACHKIK_001200"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO9082413.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13861..14520
                     /gene="yghB"
                     /locus_tag="ACHKIK_001201"
     CDS             13861..14520
                     /gene="yghB"
                     /locus_tag="ACHKIK_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO9082414.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHKIK_001202"
     CDS             complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHKIK_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO9082415.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHKIK_001203"
     CDS             15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHKIK_001203"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO9082416.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHKIK_001204"
     CDS             16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHKIK_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO9082417.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17948..18874
                     /locus_tag="ACHKIK_001205"
     CDS             17948..18874
                     /locus_tag="ACHKIK_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO9082418.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18925..19182
                     /gene="yqhH"
                     /locus_tag="ACHKIK_001206"
     CDS             18925..19182
                     /gene="yqhH"
                     /locus_tag="ACHKIK_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO9082419.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(19225..21444)
                     /locus_tag="ACHKIK_001207"
     CDS             complement(19225..21444)
                     /locus_tag="ACHKIK_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO9082420.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="ACHKIK_001208"
     CDS             complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="ACHKIK_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO9082421.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="ACHKIK_001209"
                     /gene_synonym="parF"
     CDS             complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="ACHKIK_001209"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO9082422.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(24012..26270)
                     /gene="parC"
                     /locus_tag="ACHKIK_001210"
     CDS             complement(24012..26270)
                     /gene="parC"
                     /locus_tag="ACHKIK_001210"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO9082423.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLEQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(26816..27298)
                     /locus_tag="ACHKIK_001211"
     CDS             complement(26816..27298)
                     /locus_tag="ACHKIK_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO9082424.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPIMFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="ACHKIK_001212"
     CDS             complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="ACHKIK_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO9082425.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            27895..28554
                     /gene="qseB"
                     /locus_tag="ACHKIK_001213"
     CDS             27895..28554
                     /gene="qseB"
                     /locus_tag="ACHKIK_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO9082426.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            28551..29900
                     /gene="qseC"
                     /locus_tag="ACHKIK_001214"
     CDS             28551..29900
                     /gene="qseC"
                     /locus_tag="ACHKIK_001214"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO9082427.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(29946..30278)
                     /locus_tag="ACHKIK_001215"
     CDS             complement(29946..30278)
                     /locus_tag="ACHKIK_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO9082428.1"
                     /translation="MLKHKIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLESGDNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLF
                     WGRFIFCY"
     gene            30597..31178
                     /gene="mdaB"
                     /locus_tag="ACHKIK_001216"
     CDS             30597..31178
                     /gene="mdaB"
                     /locus_tag="ACHKIK_001216"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO9082429.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            31209..31523
                     /locus_tag="ACHKIK_001217"
     CDS             31209..31523
                     /locus_tag="ACHKIK_001217"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO9082430.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(31571..33463)
                     /gene="parE"
                     /locus_tag="ACHKIK_001218"
     CDS             complement(31571..33463)
                     /gene="parE"
                     /locus_tag="ACHKIK_001218"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO9082431.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(33492..34073)
                     /gene="yqiA"
                     /locus_tag="ACHKIK_001219"
     CDS             complement(33492..34073)
                     /gene="yqiA"
                     /locus_tag="ACHKIK_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO9082432.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(34073..34900)
                     /gene="cpdA"
                     /locus_tag="ACHKIK_001220"
     CDS             complement(34073..34900)
                     /gene="cpdA"
                     /locus_tag="ACHKIK_001220"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO9082433.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYRHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(34925..35347)
                     /locus_tag="ACHKIK_001221"
     CDS             complement(34925..35347)
                     /locus_tag="ACHKIK_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO9082434.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(35348..35977)
                     /gene="nudF"
                     /locus_tag="ACHKIK_001222"
     CDS             complement(35348..35977)
                     /gene="nudF"
                     /locus_tag="ACHKIK_001222"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO9082435.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            36182..37663
                     /gene="tolC"
                     /locus_tag="ACHKIK_001223"
     CDS             36182..37663
                     /gene="tolC"
                     /locus_tag="ACHKIK_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO9082436.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            37811..38482
                     /locus_tag="ACHKIK_001224"
     CDS             37811..38482
                     /locus_tag="ACHKIK_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO9082437.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            38488..39648
                     /locus_tag="ACHKIK_001225"
     CDS             38488..39648
                     /locus_tag="ACHKIK_001225"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO9082438.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(39686..40474)
                     /gene="ygiD"
                     /locus_tag="ACHKIK_001226"
     CDS             complement(39686..40474)
                     /gene="ygiD"
                     /locus_tag="ACHKIK_001226"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO9082439.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            40617..41390
                     /gene="zupT"
                     /locus_tag="ACHKIK_001227"
     CDS             40617..41390
                     /gene="zupT"
                     /locus_tag="ACHKIK_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO9082440.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(41448..41618)
                     /gene="yqiD"
                     /locus_tag="ACHKIK_001228"
     CDS             complement(41448..41618)
                     /gene="yqiD"
                     /locus_tag="ACHKIK_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO9082441.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(41879..42532)
                     /gene="ribB"
                     /locus_tag="ACHKIK_001229"
     CDS             complement(41879..42532)
                     /gene="ribB"
                     /locus_tag="ACHKIK_001229"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO9082442.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(42636..42784)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            42906..43196
                     /gene="ubiK"
                     /locus_tag="ACHKIK_001230"
     CDS             42906..43196
                     /gene="ubiK"
                     /locus_tag="ACHKIK_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO9082443.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT        10759 a        11546 c        10807 g        10122 t
ORIGIN      
        1 aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg
       61 ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact
      121 ggcccagtag atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga
      181 taccacatac ttcgacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc
      241 ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt ttgacgatca
      301 gcgaatcgcc ggtatcgaca cgcaaccgct cgccgtgaca gagcggacac tgcgcatcgt
      361 gctgatgaat ctccaccacc tggctgcaat cccagcacca agcctgggcg ggtttataga
      421 cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac
      481 ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc
      541 gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagag
      601 acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt
      661 ggtacatccg gattcgtgac tgacagcgaa aggatcattc gtgcgcagtc atccgtcagg
      721 cgttggccct cttcaatcga catgctgtgc gaaagcggcg acatcagcga acaggagaga
      781 tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tagtgccata cggcagttgc
      841 agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata
      901 actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta
      961 aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc
     1021 cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct
     1081 atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgccagatt cacgcagcgc
     1141 agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat
     1201 gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcacc
     1261 ggtgaagcgc agggccgaca atacgtcggc caggccaagc tgatgcggag agattttgtt
     1321 ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg gcgtgttctt
     1381 tttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag
     1441 ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg
     1501 ctctaacgct tcgacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac
     1561 ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcggca
     1621 tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact
     1681 tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc gtaaggaccg
     1741 acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac tgcctggtag
     1801 ttgctgatga taccgtcttt aatcaccatc cagtgagaga gcataccgcg cggcgcttcg
     1861 aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac aaaggtggtg
     1921 tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc
     1981 aattcacagc agtgaacggt acgaccaata atacggccca gcgtggagtg cagctgtgcc
     2041 acttccagcg tgttgccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc
     2101 tgggtagatt ctcgacctgc cgccagtttc accagcatat tagccagcgg acccacttct
     2161 accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa
     2221 ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc gtctttatac
     2281 caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg
     2341 atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg
     2401 ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg
     2461 cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc
     2521 tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc
     2581 aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc caggttctga
     2641 atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc
     2701 agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgct
     2761 gggtgacccc agtagccatt agcgaaaata cccaactgac cgctggcaac cagatctttg
     2821 atcttgttct gaactttagt gaactcttcc ggactgttca gatgccaggt cgaaacgcct
     2881 ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat gtccacccag
     2941 tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga
     3001 atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc
     3061 gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc
     3121 atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta
     3181 ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc
     3241 ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat
     3301 gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga
     3361 tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg
     3421 ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg
     3481 ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca
     3541 gcttcgccac acgcagaacc accagcggga agagcatcag caggacttca atccagaaca
     3601 tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc
     3661 caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttgacga
     3721 acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc gaaccttcaa
     3781 agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt
     3841 catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagt gaccccattg
     3901 aagactggtg catggtcggc aacagcgcgc caagtgcaat gatgaagaac atcaccttgt
     3961 tcagacgctt gagagaaacc ttccagccca gacgttcaaa cagtgccgga gcaaactcca
     4021 gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt accgagttca
     4081 cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag
     4141 tgatcgacaa gccccccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca
     4201 gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac
     4261 aggcaaagcc ggtaccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc
     4321 cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac
     4381 agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg
     4441 gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga
     4501 cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg
     4561 gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg
     4621 agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg
     4681 ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc
     4741 gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc
     4801 gccatcagct cttcacgcgt accaaaaatt accgcgcccg ccgggcacac ttctacgcag
     4861 ccaggcaaac cgcctttatc gagacgttcc acacctttct ggttgcacaa ctcgcactta
     4921 tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtatgg gcaggcgacc
     4981 atgcagtaac ggcaaccggt gcacacatct ttgtcgtaat ggacaatgcc ggttttcgga
     5041 tcttttttca gcgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata
     5101 cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg
     5161 ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag
     5221 gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa
     5281 gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc
     5341 gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg
     5401 caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt
     5461 tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca
     5521 gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag
     5581 cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga
     5641 tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga ccaatcgcta
     5701 ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg
     5761 gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca tcaccgaact
     5821 ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac
     5881 ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg ccgtaagtga
     5941 tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga
     6001 taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag
     6061 ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct gctgcgcctt
     6121 ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa taaataccgt
     6181 tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg tacttctcaa
     6241 gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat
     6301 actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca
     6361 gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg
     6421 ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg
     6481 ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg
     6541 tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca
     6601 atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga
     6661 tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta
     6721 aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg
     6781 caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc
     6841 atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc
     6901 gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt
     6961 cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa
     7021 atggttattc atcgttcacc tcattcgcta tatatcgcca tatatagcga tattttaggc
     7081 aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa tccagtaaga
     7141 gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat
     7201 cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg
     7261 gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc
     7321 agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct
     7381 tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg
     7441 gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca
     7501 ctttgcctgc cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca
     7561 tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat
     7621 aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc
     7681 gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc actaccacgg
     7741 ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac
     7801 catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct
     7861 gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc
     7921 ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc ccgtgagcgt
     7981 aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg
     8041 ttgcactcct caactggtgt ttttttaagc aaagcataag cacgtgtttt tgcccagttt
     8101 ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca
     8161 ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg
     8221 ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc
     8281 gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct
     8341 gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc
     8401 tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg
     8461 cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc cttacggctc
     8521 tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct
     8581 tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac
     8641 cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc
     8701 ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct
     8761 tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct
     8821 ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt
     8881 gctgaccgga aaatatctca acggcattcc gcaagattca cggatgtatc gtgaagggaa
     8941 taaagttcgt ggtctgacac cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt
     9001 attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt taagctggtt
     9061 gttgaaagat gatcgcgtga cgtcggtatt gattggtgcc agccgcgcgg agcaactaga
     9121 ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga
     9181 tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt
     9241 taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt
     9301 caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca
     9361 tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtagg tgaatggaag
     9421 aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca
     9481 gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac
     9541 cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca
     9601 acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg ttaataatct
     9661 cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg
     9721 caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc
     9781 gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc
     9841 aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct
     9901 tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga
     9961 tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac
    10021 aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc gagaaaacct
    10081 atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa
    10141 taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc
    10201 ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca
    10261 aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg
    10321 aagcgcacaa ggcgttaggc gggctggata ttatggcgct ggtcgccggg aaacaggttg
    10381 ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg
    10441 ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagta
    10501 tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg
    10561 ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag
    10621 gtattcgggt gaatattgtc gcgccaggcc ctatctggac ggcactgcaa atttccggcg
    10681 gacaaacgca ggataagatc ccgcagtttg gtcagaaaac gccgatgaag cgtgcggggc
    10741 aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca
    10801 ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt
    10861 gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa aacgccttat
    10921 ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc ctgataagcg
    10981 tagcgcatca ggcattgagc acctaatgcc ggatgcggcg tgaacgcctt atccggccta
    11041 caaacgcacg aagattcaac atgttgcaat agatattgta ggcctgatga gcgtagcgca
    11101 tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac ctatcttcag
    11161 gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt
    11221 atccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt
    11281 aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt ctgccttcac
    11341 cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt
    11401 caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca gcagaaccaa
    11461 catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt
    11521 ttcgttaaga tgcattgcca tcggatatta tcctgcgcgt aatttttgtg cgacacgaac
    11581 cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca acaatacctg
    11641 cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa atacgttata
    11701 gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca gagcttctgc
    11761 gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac caataaagct
    11821 gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg cgccgatggt
    11881 tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc gacgctccag
    11941 acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag
    12001 ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc
    12061 gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct cgcgcttaag
    12121 gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc aagtgactac
    12181 ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt gctgatacat
    12241 accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc
    12301 aagtcacaaa atctgcgcat aataatatca aaatgacgtc gaattgatag tcgttctcat
    12361 tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag
    12421 tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg
    12481 cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc gacatggcgg
    12541 acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac actctcggcg
    12601 cggtaaatag cgtgattcag cgcgcttctt cgctggtctt tgacagtgtg gaagccaaaa
    12661 agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc ggaacgttaa
    12721 cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgcgcgctat
    12781 ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa cagggcgatc
    12841 atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca
    12901 gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc
    12961 atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggctccatc accatggaag
    13021 tccacgacgt tccggcgatt gttgccgccg tacgcagtgt ggtgccggat gccatcatta
    13081 tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg
    13141 tttctattca agccgccacc aaatatctgg ttgggcattc agatgcgatg attggcactg
    13201 ccgtgtgcaa tgcccgttgc tgggagcagc tacgggaaaa cgcctatctg atgggccaga
    13261 tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt
    13321 tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa catccgcaag
    13381 ttgcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc tggaaacgag
    13441 actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc aataatgaag
    13501 agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg tggggcgggt
    13561 atgaatcgtt gatcctggcg aatcaaccag aacatatcgc cgccattcgc ccccaaggcg
    13621 agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc
    13681 tgattgccga tctggacgcc ggttttgcgc gaattgtata acattgccac ttttggacaa
    13741 ttttgcagac atttaattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc
    13801 ggacgaatca ggagtacaat aggcagataa aggcttaaac gctgttccac aggaaagtcc
    13861 atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg
    13921 gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac
    13981 ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc aggcgcattg
    14041 attgcccaag gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt
    14101 ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa aacggtgaaa
    14161 ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat gtttgaccgc
    14221 cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca
    14281 accatggcgg gaatttccgg tctgccaaac cgccgcttcc agtttttcaa ctggttaagt
    14341 ggattgctgt gggtcagcgt ggtaaccagt tttggctatg ccttaagtat gattccgttc
    14401 gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta
    14461 accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga
    14521 cgattttccc cgttcccggc tgctgtaccg ggaacgtatt taattcccct gcatcgcccg
    14581 cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa
    14641 ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctggccttca tgccgtcatg
    14701 gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt
    14761 gctggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg cttctgccgc
    14821 cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca gcacgcggct
    14881 aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc
    14941 ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac gcgcatccag
    15001 tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt
    15061 ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt cgccaatgtc
    15121 catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg ccagcggcac
    15181 ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca gcaggtattc
    15241 attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc cggaaaagag
    15301 aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa
    15361 caaacgtaca tcgggcaaca gttctgacaa actattttct ttaattttca gtttattaac
    15421 tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt tcttaggaat
    15481 aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct ggaggaatag
    15541 gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac aatgaaaaac
    15601 atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag taatgaacaa
    15661 ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg
    15721 cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa
    15781 aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg
    15841 cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tggttcgcga
    15901 acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt
    15961 tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg
    16021 cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg
    16081 ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt
    16141 ccattctgcc catgttcagc cggtatttgc cgtgctcgat ccggtttata cctacaccct
    16201 gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg tggaacagta
    16261 tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac
    16321 gctgatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa
    16381 cgtcatgtgg gcggcgacgc aggcgctgaa cggtttgatt ggcgctggcg taccgcagga
    16441 ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca
    16501 aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa
    16561 gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgatg atgagcgtat
    16621 tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct
    16681 ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca
    16741 cggcatgacc caactgggcg aaaatcatga cattacgttg gatgtcagcc gccgtatata
    16801 cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat ttcgtccaga
    16861 cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa ggaggaacgt
    16921 atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gctgggactg
    16981 ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg
    17041 ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc
    17101 ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac
    17161 gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca gcttgattat
    17221 atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg
    17281 aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc
    17341 cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag
    17401 atcgaacttc atccgctgat gcaacaacgc cagctacacg cctggaacgc gacacacaaa
    17461 atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa
    17521 gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt tatccgctgg
    17581 catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg tattgccgaa
    17641 aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc
    17701 gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaaca tgcaaattct
    17761 cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc
    17821 ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc atcatgcaga
    17881 gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac ggagaggtga
    17941 aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta ccgtacacgc
    18001 acagcccccc tcactgaccg tagaacaaac agtccggcat atttatcaga actataaatc
    18061 agatgccact gccccttatt ttggtgaaac cggagagcgg gcgataactt ctgcgcgtat
    18121 tcaacaggcg cttaccctga acgacaatct tacgctgccg ggcaatattg gctggctgga
    18181 ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgctagaaa gcgttgcgat
    18241 aacccaaact gacgccgatc atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga
    18301 taaagaaaag accacgcaga cactgaaaat ggtggcggaa aatggtcgtt gggtcattga
    18361 cgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac
    18421 gctggccgct ttagcttcgt tgcaaaaaga acagccggaa gcctttgttg ccgaactctt
    18481 tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact cttaccgcca
    18541 ggcggttaat gccttctata aaaccacctt caagacggcc aataatcccg atgaagatat
    18601 gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag agtcgctatt
    18661 ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc gtattcatgt
    18721 tcgttttacg ctgaccaatg gcaacaacga agagcaagaa ctggttttac agcggcgcga
    18781 aggcaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac ttaagcagat
    18841 tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca attagccgga
    18901 acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc tgttgttctg
    18961 gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcagct ggcgagcaat
    19021 gtgcaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc actacgccca
    19081 caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga tgctcaggac
    19141 tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa tccccggcag
    19201 catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc gtttcttcgc
    19261 accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag caggcgtctg
    19321 acgctgggtc gccatcggcg tatgtttcgt caacgccgga cgggtattgc ggttctggcg
    19381 acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc gacggctgcc
    19441 aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc agtttgccgg
    19501 atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct tcggtacgaa
    19561 gacgtcttcg ctcttataac caatcttcgc cagcgggttt ttcccggtgt aatacatggt
    19621 ggttgagttc gccagcggcg atgggtagaa gttctgtacc tggtcgagac ggaaacgatg
    19681 ctttttcagc cacagcgcca gattcaccat atcttcatca cgcgtaccgg ggtgcgcgga
    19741 gatgaaatag gggatcagat actgctcttt acctgcctgt tttgagtaag tatcgaacag
    19801 ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgata acggcccttc
    19861 ttcggtatgt tccggggcaa tcttcagata accgccgacg tgatgggtcg ccaactcttt
    19921 gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga tgaggatttt
    19981 tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt cgtggttagt
    20041 gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag tttgttcagc
    20101 gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga gatcggaaat
    20161 tacgcccgta aaaccaggaa cggtgtcgcg gatcgcttcg atctcattaa tgatcgaatc
    20221 ttccgaacgg ctctgaataa tgcgcccttc gtgctcggtg atagaacaga aagagcagcc
    20281 gccaaagcag ccgcgcataa tgttgaccga gaaacggatc atttcgtaag ccggaatacg
    20341 ggcattgcca taggccggat gcggcacgcg cttgtacggc agcgcaaaaa cgctgtccat
    20401 ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt caccgtgttt
    20461 ttgcatcaat gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc gcgaagcatg
    20521 agcgtacagg actttatcgc ccttcacttt ctcgaaagaa ggcagcaata cgtaggtttt
    20581 ttcccacggt ttcgggcgcg gcggctgcac ggttacagct ttcgcttcct gctttttcgg
    20641 cgcaacaggt ttgttatccg cgcacggcag atcttcgcca tacggatgcg ggattgggtc
    20701 gattttccct ggggtatcaa gacgagtaga atccacaccg ctccagccag gcagcgcctc
    20761 ttttacgata attgcggtat tacgcacatc gcggatttcg ctaattggct cgcccatcgc
    20821 cagacgatgc gctacttcca ccagcggacg ttcaccgtta ccaaacatca gcatgtcggc
    20881 tttcgaatcc accagcacgg aacggcgcac ggtatcggac cagtaatcat aatgcgcggt
    20941 acggcgcaga ctagcctcaa taccgccaag gatcaccggc acatctttcc acgcctcttt
    21001 acaacgctgg gtataaacca gtgtggcgcg atccggtcgc ttacccgcga cgttatccgg
    21061 tgtgtaggca tcgtcatgac gtaaacggcg atcggcggta tagcggttga tcatcgaatc
    21121 catgttgcca gcagtaacac caaaaaacag attcggttta cccagacgca taaagtcgtc
    21181 tttgctgctc cagtccggct gggcgatgat cccgacgcga aagccctgcg cttccagcat
    21241 acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc cagtaaccaa
    21301 aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca tcggcaaaaa
    21361 cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt cgcgatccgg
    21421 ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat aatcaaaggc
    21481 cggggattat aagccggaac gaaagagaaa tcgaaaggta ttccatactc gccctcctcg
    21541 ggcgagtatg aagattacgg taccggattg accaacagtt gcccaatcga gccacggtcc
    21601 gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga aggctgaccg
    21661 aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca gcctctgtct
    21721 tccgggaacg gcatcgcgcc attcacgtta cggatctgga acatcacgcc ttcaatatgg
    21781 aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg ctgcgcggtg
    21841 acatcaatac ggttgacgtc ccacagctgt ccattaatac ccgggtcatc acccagacta
    21901 atatcgcgac tgcgaatcgg cgaaccggcc atgatttcag ttggcagcaa gcgcatcgga
    21961 agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac cagggtagaa
    22021 accaaaatgc tggatggctc aaagaagcca cgaatacgat caacaatgct cgccgcttcg
    22081 ccacaggtga tcgacacttc atcaccgttg ctcatatcca ccagaatctc gcggcgctcg
    22141 cccggtgcca gcgaaagttg cttcaccgat acaggagcag gcaggaatcc ctgatcgcca
    22201 gaaatcacat gtaacgggcg accatcgctc atctgtagtt gatagcgacg agagttcgac
    22261 gcgttcagca gtcgcaagcg cacccagcca cgcgagactt caacgtacgg gctttgtaca
    22321 ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt gtattctggc
    22381 gtaccaaagt tatccagccg tttatcctgg ataatgaccg gaaaatcatc cacaccataa
    22441 tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat tccggcaagg
    22501 ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca cagagtagct
    22561 gcgttctggc gaatgggcag tacgggtgcc cagtcagcgt ttggcgacat catccgtgcc
    22621 ggaccgccca tcagcggacc tggtacctgt agcccggcca ccgtcattga gacattttct
    22681 gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg gatagtcggc
    22741 cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt aaatgaccag
    22801 tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag tagcggcgga
    22861 acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc gcctgcacaa
    22921 agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt tgctccatgt
    22981 aaaactggct aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac agattgccga
    23041 cttaaacttt tccggcggct tcgcgttctg cgacttcttt atcgagctcg gcgatttttt
    23101 gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg ccatactgac
    23161 tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt cgattaagat
    23221 taatcttatt cgaagttgta gagacgcaca cgggaataat cgggacgccc gccgcaattg
    23281 ccgcgtgaaa tgctccagtc ttgaacggta gcaggccgcg accacggctg cgggttcctt
    23341 ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcacgact tccgcaatgg
    23401 tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt aaccagtaca
    23461 actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc gtcggcggtt
    23521 gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg atatagatag
    23581 cattgccgta gctttcagca tctgcaggtt tgcggcactc aactttcagg ccgaacagcg
    23641 gcgcaagacg gccaaacata tgcccaaagg tggccacatg tttcgggtta cgcgggctga
    23701 aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcacg gtaataataa
    23761 gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat tgtacgtcac
    23821 ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat tgttaatcgc
    23881 aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg ttgaatacgc
    23941 tgccggatga cgacttaacg tttcatccgg cgttccttgc aagcgggagg aaacaggccc
    24001 tccccggcat attactcttc gctatcaccg ctgctggcac ggcgaggaga gtcgatctca
    24061 acacgatcga tacgctgcaa accgcgcatc aacgtaccgc ggcgtccacg ttcgccagtg
    24121 actttctgta actcttccgg gcgcagttta attttgcgtt tcccaacatg aatggtcagc
    24181 gtgctttgcg gcggcagaac gtacaattgc gccagaccat cttctccacg cgcggcttct
    24241 gccgatggaa tgttgataat cttgttgcct ttgcccttcg acagctgcgg cagatcgctt
    24301 accgggaaca tcaacatacg gcctgcctga gtgattgcca gcagcatatc ggaagcatct
    24361 tcaatcacca ccggcggcat aacatgggca ttttccggta aggtgatcaa agccttacct
    24421 gcacggttac gcgccaccag atcgttaaag gtgcagacga aaccgtaacc cgcatcggaa
    24481 gccatcagca gtttctgatc gtcgctttcc atcagcatat ggtcaacggt cgccccaggc
    24541 ggcaacgtta atttgccggt gagtggttcg ccctgaccac gcgccgacgg cagcgtaatc
    24601 gggtcgatgg catagctacg accggtggaa tcaacaaaca ctaccggttg gttgctctta
    24661 cctttcaccg ccgctttgaa gctatcaccc gctttataat tcaggcccgg cgcgtcgata
    24721 tcatggcctt tagcgctgcg tacccagccc atctgcgaca gcacaatggt gacaggttca
    24781 gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt cgcgttcctg caacggcgaa
    24841 cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt ctttcttcag caggttattc
    24901 attttacgct cggaagccaa aatgccctgc aactggtcgc gctctttttc cagctcactc
    24961 tgctcaccgc gaatcttcat ctcttccagt ttggcaagat gacgcagttt cagttcgagg
    25021 atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca tcagcgccgg tttcggttca
    25081 tcttcgttac gaatgatctc aatcacttcg tcgatgttga gaaacgccac cagcaaacct
    25141 tccaggatat gcaggcgctt gaggactttc tccagacgat agttcagtcg gcggcgcacg
    25201 gtatcgcgac ggaacaccag ccattcggag aggatttcca gcaggttttt caccgccgga
    25261 cgaccatcca gaccgatcat attgaggtta atgcgatagc tcttttccag atcggtggta
    25321 gcgaagaggt ggttcatcac ctgatccata tccacgcggt tggaacgcgg cacaatcacc
    25381 agacgggtcg ggttctcgtg gtcagattca tcgcgcagat cgtcaaccat cggcagcttt
    25441 ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg cacctgaaac ctgatgcggt
    25501 aatgcgctga taaccaccgc gccatcttct ttcttccaca ccgcgcgcat acgcactgaa
    25561 ccacgtccgt tctcgtagat tttacggatc tcggcgcgcg aagtgataat ttccgcttca
    25621 gtcggataat ccggcccctg cacgatatcc agcagctgct cgagcgtggt tttcggctgg
    25681 tcgattaatg cgattgccgc ctgagctact tcacgcaggt tatgcggtgg aatatcggtc
    25741 gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa tgtttggcag acgagcaggc
    25801 agcattttcg gctcctgcaa agtgccgtcg aagtttggca cccagtcagc cgtcccctgc
    25861 cccagctcgc tcaatagcag ctcggaatat ttcgacaacc gggattcggt gtaacgcatt
    25921 gccgcgaacg atttcggatc gtccggcgcg ccccagttcc cctgaccatc aaccagcgga
    25981 taacggtaag agaacggctg cgccatcagg accatcgctt cataacaggc gctatcgccg
    26041 tgcggatggt atttacccag tacgtcaccg acggtacggg ccgatttttt aaatttggcg
    26101 ctggcattca ggcccagttc agacatcgca tacacaatgc ggcgctgaac aggtttaaga
    26161 ccatcgccaa taaacggcaa cgcacggtcc atgatcacgt acatggagta gtttaagtag
    26221 gcgttttccg taaattcatg tagcgcaagg cgctctgcca tatcgctcat taattctgat
    26281 tcctcaactt attcgccagc cttcaaaggg gcaatattgc cggagatact acattatctg
    26341 ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc ggacgtgctc cgacggcgat
    26401 aaaggcatca atgtgccttg atatacaact ctttgctgta gacttgatcg gcatagaggg
    26461 cagcacaagg ttgcgcccat gccacaccac tcggcatggg ggtccgctcc gggcgattcg
    26521 agaagttgag gttatgcgag tccagggaag cctaaagcat caaagtagtc gactgtaagt
    26581 atacggttca gcccagttta gctgtgtctc caccagtgcc ccgcgcctcc tgccataagt
    26641 gcggcttgct catggatacc cccgagacgg cttacggcgc accggataat aaatttcgcg
    26701 tatacatggt attccttacc ggagaaatta taagcccaac aacaatacgc ctgtggcatt
    26761 gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagatt ccccatcacg
    26821 ccaacggcac ataaacatcc gtttccagtc gctgctctgt caccccttcg gcaagattgg
    26881 tgtagtgaaa cataatcggt gctttacgca ttttctcgcc gcttgcaggc agccagtggc
    26941 gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc gccaacgtgg cgggccacgg
    27001 cataacgtcc accggtaagt tcaccattgc tcacaccata acgattatcg ggaatcggtt
    27061 cgctaacgct gccgcagata tcaaagcgaa acgcttccgg tgcggtggtg gcagggtcgt
    27121 cccaggcgac gccaaaagtc tggctttggt taacaggaga aagccccgtc tctttacgcc
    27181 acatgataaa tttcgccaca ctgtagttga gcaactcagg gctacagcgg tgcggcaaca
    27241 tcgccacagg gattgatggg aaatcgataa tgtttacatc cagtgtcagg tttgtcattt
    27301 tgcctccctg cgaaattatt cccgggaacg cgtcccggga gcggtaacaa ttacggattt
    27361 actttgcgga tctgtttgac gtcaatttca acagaattcc agtctttatc gacttcaccc
    27421 tgaatctcaa ccgtatcttt cggcgtcacc gtcacaccgt tccagcgttt gtggtcgata
    27481 tcaacattga tagtaccgct ggcatcttta aacacgtaga gatcgtcaga gatgcgttca
    27541 acgatattgc cgcgcagggt tacccaggtg tcgtcacgca gggattttgc gctttctacg
    27601 gtcgttacgc tgccgttcgg cccctggaat cctccggcct gactttgcgt tgccgatggg
    27661 ccagaaaaac cgccctgctc tgctgccatc accggtgcgc tgcacagggc cattactgcg
    27721 attactgctg cgaatttttt catgtttatt actcccttta atgtctgttt ccgagcattt
    27781 aacaagatag tccttaacaa cttcttaagg gaaaaaaata aaatttagtg ctgtacagag
    27841 cgcgttacaa cacggtttac tggcagcaaa tacggttatc gcagggatga aaaaatgcga
    27901 attttactga tagaagatga catgctgatt ggcgacggca tcaaaacggg ccttagtaaa
    27961 atgggtttta gcgtcgactg gtttacacaa ggtcgtcagg gaaaagaggc gctttatagc
    28021 gcgccttatg atgcggtgat cctggattta accttaccag gcatggatgg tcgcgatatt
    28081 ttgcgcgaat ggcgagaaaa aggtcagcgt gagccggtac tgatcctgac cgcgcgcgat
    28141 gcgctggagg aacgtgtaga agggctgcgt ctgggagctg acgattatct gtgtaaacct
    28201 tttgcgttga tagaagtcgc cgccaggctg gaagctctga tgcgccgaac caacggccag
    28261 gccagcaacg agctgcgcca cggtaacgtc atgctcgacc ccggcaaacg tatcgccacg
    28321 ctggctggcg aacccttaac actgaaacca aaagaatttg ccctgctgga attactgatg
    28381 cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag agaaactgta tacctgggac
    28441 gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc atctgcgacg caaactcggt
    28501 agtgatttta ttcgtaccgt gcatggtatt ggctacacat taggtgagaa atgaaattta
    28561 cccaacgtct tagtctgcgc gtcaggctga cgctaatctt tttaattctg gcctcggtga
    28621 cctggctgct ttccagcttt gtcgcctgga aacaaacaac ggataacgtc gatgaattgt
    28681 tcgacaccca actgatgctg tttgccaagc ggttaagtac gctcgatctc aacgaaatca
    28741 acgcggcgga tcgcatggca cagacgccaa ataaattaaa acacggtcat gttgatgacg
    28801 atgcgctgac ctttgccatc tttacccacg acggcagaat ggtccttaac gatggcgata
    28861 acggagaaga tattccttat agctatcaac gggaaggttt tgctgacggg caactggtgg
    28921 gcgataaaga tcaatggcgt tttgtctgga tgacctcacc tgatggcaaa tatcgcatcg
    28981 ttgttggtca ggaatgggaa taccgtgaag acatggcgct ggcgattgtt gccgggcaat
    29041 tgatcccgtg gctggtcgca ctgccgatta tgttaatcat catgatggta ctattgggtc
    29101 gtgaactcgc gccgctgaac aaactggcgc tggcgctgcg tatgcgtgac cctgactcgg
    29161 aaaaaccact aaacgcgact ggcgtaccca gcgaagtgcg tccgttggtt gagtcgctaa
    29221 atcaactgtt cgcccgcaca catgcgatga tggttcgtga acgacgcttt acctccgacg
    29281 cagctcacga actacgtagc ccgttaacgg cgctgaaagt gcaaaccgaa gttgcgcagc
    29341 tttctgacga tgatccgcaa gcgcggaaaa aagcgctgct tcaattacat tacgggatcg
    29401 atcgcgctac tcgtctggtt gatcaactgc tcactctatc gcggctggac tcactggata
    29461 accttcagga cgtcgcggag atcccgcttg aagatctcct gcaatcgtcg gtgatggata
    29521 tttaccacac ggcgcagcag gcgaaaattg acgtgcgact gacactcaat gcccacggca
    29581 tcaaacgcac cggacaaccg ctattgctaa gtttgttggt gcgaaatttg ctggataacg
    29641 ccgtgcgcta cagtccacag ggcagcgtga tagacgtcac gctgaatgct gataatttca
    29701 tcgtgaggga taacggcccc ggtgtgacgc cagaggctct ggcgcgaatt ggcgaacgct
    29761 tctatcgccc ccccggacaa accgctaccg gcagtggact tgggctatcg attgtccagc
    29821 gaatcgccaa attgcatggc atgaatgttg agtttgggaa tgcggaacaa ggtggatttg
    29881 aggcgaaggt aagctggtaa cttctctgtc tatgagagcc gttaaaacga ctctcataga
    29941 ttttactaat agcaaaatat aaaccgtccc caaaaaagcc accaaccaca aaccagacaa
    30001 ataccgagcg agaatacata tacccaaaac tcgaacattt cccgcataaa gagttttctt
    30061 aagataagaa taataagtgg cgtaagaaga aaaaatgctg caatcattgc ttgcaatata
    30121 cgatcttcgc ctgaaacgga aggtatactg caaatattgt ctcccgactc aagcatccag
    30181 tcatacttat tgaaagcaaa aaaagcatct attaaccaat aagtaagcat tatgtataac
    30241 agagcttcaa atattgtttg aattttatgc tttaacatat atcagtacgc tcataaaaat
    30301 ccatttataa ttatcaacat gacacgttga tattttcatc ttttacttat tcaaagccat
    30361 attttcaatc ccaaaaaaaa ccgaaccaaa cgtgtaaatt attccggtgc gagtaagaat
    30421 tatgaggaat ggctatcagt attgtcattt tcagaaaata tttatcctgc atcggtgagt
    30481 cagagcaaga tcagactttt gctaaattcg caaaagactt tgcacatttt gctaatttca
    30541 ccgtaccgct ctgtgacgta ctatagtcgg caaacgtctc accttgaggt taaaaaatga
    30601 gcaacatcct gattatcaac ggcgcgaaaa aattcgccca ctccaatggt caactgaacg
    30661 acaccctgac cgaagtcgcg gatggcacac tgcgcgacct cgggcatgat gtccgcatcg
    30721 ttcgcgccga cagcgactac gatgtcaaag cggaagtaca aaactttctc tgggctgatg
    30781 tggtgatctg gcagatgcca ggctggtgga tgggcgcgcc gtggacagtg aaaaaatata
    30841 ttgatgatgt attcaccgaa ggtcatggaa cgctgtatgc cagcgatggc cgtacccgca
    30901 aagatccgtc aaaaaaatac ggttccggcg gcctggtaca gggcaaaaaa tatatgcttt
    30961 ctctgacctg gaacgcacca atggaagcct tcaccgaaaa agatcaattc ttccacggcg
    31021 ttggcgtcga cggtgtgtat ctgccgttcc ataaagcaaa ccaattcctt ggcatggaac
    31081 cgctgccgac atttatcgct aatgacgtga taaaaatgcc tgatgttccc cgctatactg
    31141 aagaatatcg caagcatctt gtggaaattt ttggttaact agagctcagg ctttagaagg
    31201 agttaaccat gcttaccgta atcgcagaaa tccgtactcg tcctggtcaa catcaccgtc
    31261 aggcggtatt ggatcagttt gctaaaatcg ttccaaccgt actgaaagaa gaaggttgcc
    31321 acggctatgc gccaatggtg gattgcgctg ctggcgtgag tttccagtct atggcaccgg
    31381 attctatcgt gatgattgag cagtgggaaa gcatcgcgca tcttgaagcg catctgcaaa
    31441 ccccgcacat gaaggcgtac agcgaagccg taaaaggcga cgtgctggag atgaatatcc
    31501 gcatcctgca acctgggatt taaacctgcc ttgtttgccc ggccatcctg accgggcaat
    31561 gttctttcct ttaaacctca atctccgcca tgtcgccttt ctcttgcaac cagttgcggc
    31621 gatcttccga gcgtttcttc gccagcagca tatccatcat cgcgtcagta cgctgatcgt
    31681 cttcatcatc gatagtcaac tgcaccagac ggcgagtgtt cggatcaagc gtggtttcac
    31741 gcaattgcat cgggttcatt tcccccagac ctttaaaacg ctggacgttc ggcttgcctt
    31801 tcttgcgttt taattgctca agtacgccct ctttctcttc ttccgtcagc gcgtaataaa
    31861 cctctttccc gagatcaata cggtagagcg gtggcagtgc gacgtaaacg tgaccgtgtt
    31921 tcaccaacgc gcggaagtgt tttacgaaca aagcgcagag cagcgtggca atgtgcagac
    31981 catcagagtc cgcatccgcg aggatacaga ttttgccata acgaagctgg ctcagatcgt
    32041 cgctgtcagg atcgataccg atcgctaccg aaatatcatg cacttcctgc gaggccagca
    32101 cttcgtcgga agaaacttcc caggtgttaa ggatcttacc tttcagtggc atgatcgcct
    32161 gatattcgcg atcgcgcgcc tgcttggcag atccgcctgc ggagtcacct tccacaagga
    32221 acagttcggt acggttaaga tcctgcgcgg tacagtcagc cagtttgcca ggcagtgctg
    32281 ggccgctggt cagctttttg cgcaccactt ttttagccgc acgcatacgg cgctgggcgc
    32341 tggaaatcgc catctccgcc agcagctccg ccgcctgaac gttctggttc agccacagga
    32401 taaaggcatc tttcaccacg cctgaaacga atgccgcgca ttgacgcgaa gagagacgct
    32461 ctttggtctg cccggcaaac tgcggatcct gcattttcac cgacagaaca taggcgcagc
    32521 gatcccagat atcttccgcc gacagcttca cgccgcgcgg cagaatgttg cggtattcac
    32581 agaactcacg catcgcgtcc aacaggccct gacgcagacc attaacatgg gtaccgccct
    32641 gcatcgttgg gataaggttg acgtagcttt cggtcagcag ttcaccgcct tccggcagcc
    32701 acagtagcgc ccagtccaca gcttcagtat cgccagcgaa attaccgata aacggttttt
    32761 ctggcagcgt cggtaaaccg tttaccgctt ccgccaggta atcattcaga ccgtcctgat
    32821 agcaccagcg ctgttcggtg ttgttgatct catctttaaa ggtaatctca acgccggggc
    32881 acagtactgc tttggccttc agcacatgcg tcaggcgtga aacagaaaaa cgcgggctgt
    32941 caaagaaggt ttcatccggc cagaagtgca cgctggtacc ggtattgcgt ttaccgcaag
    33001 tgccgacaac ctgtaaatcc tgcacctttt cgccattttc aaaggcgatg ttataaacct
    33061 gaccatcgcg gcgcacgtta acttctacgc gcttcgacag ggcgttaacc accgaaatcc
    33121 ccacgccatg caggccgcca gagaactggt aatttttgtt agagaattta ccgcctgcat
    33181 gcagacggca aagaatcagt tcaaccgccg gtacaccctc ttccgggtga atatccaccg
    33241 gcatcccgcg cccatcgtca ataacttcta acgactggtc agcatgtaag attacatcca
    33301 cgcgttttgc gtgacccgcc agtgcttcat cgacactgtt atcaataact tcttgcccca
    33361 aatggttagg gcgagtggta tcggtataca tccccggacg gcggcgaacc ggctcaagcc
    33421 cggtgagtac ctcaatggca tcagcgttat aagtttgcgt catggtttaa gttagcaatt
    33481 cgagttgatc gtcagagatg gtgcagacca agaaaatcga cgatcgggtt gaaataatct
    33541 tcgaagcccg tgaatgcgtg gttgccgcct tctatgacag tctggcggca ggaagcgtag
    33601 tacgccaccg cctggcggta atccagcact tcatctcccg tctgttgcag cagccagatc
    33661 aaatccggcg cttccagcgg gtcaatctgc atgactttga gatcgtaaat atggcgtgac
    33721 tctagcacat attgctgccc ggtgtagggg ttctcgttct gaccgagata gtccgtcagc
    33781 agttcaaacg ggcgcaccgc cgggtttacc accactgcgg gcagcataaa acattgtgac
    33841 aaccaggtgg cgtaatatcc ccccagtgac gaaccgacaa tacccagcga atcgccgcca
    33901 tgttccagga caatggattc cagcagctct gccgcgtcgg aaggatacgg cggcaactgc
    33961 ggaatgatca tctcaacgtc agggtgatgt tccgccagcc agttttttaa caagctcgct
    34021 tttgcagagc gcggcgagct gttgaaaccg tgtaaataaa gaagcgtaga catcagtagc
    34081 cttctgaagc ggtatcaggt tggaaacgtg tgtccgccag gcgatgcacc tcggtggtca
    34141 gcgtgccatc agcatgtaac tcgagagtac gccagccggg cgcgatggtg tccagcgtaa
    34201 agttggaaca gtgcggctta aactgcacac aggtcgacgg cgttgccagc aggcggcgac
    34261 cgttccagtc gagatccagc tcctgatgaa tatgaccgca cagcaagtat ttgacgtgcg
    34321 gaaacttcgc cagcacggta tccagttcgc ccgcgttacg caggctgtgt tgatcaagcc
    34381 aactacaacc cgcaggtagc ggatgatgat gcagcagcag caacgtatgg cgttctggcg
    34441 catcggccag tttacgttcc agccactcaa gctgaaactc gctcagctca ccgtgcggca
    34501 cgccaaacac ctggctatcc agcaacagga tttgccattg ctcaccaata aacacccgct
    34561 tcgccgggga gatacccgca tcctgtaacg cgctgtacat cgcgggctgg aaatcgtggt
    34621 tgccaggcag ccagacgcag ggcgcacgaa aacttgcgat gccttcagcg aaatgccgat
    34681 aggccgcaga ggattgatcc tgcgctaaat cacctgtcgc gacaatcagg tcgaattcgt
    34741 gctggtgtgg acgaatcgcc tccagcaccg cctggtagct ttcccaggta tttaccccta
    34801 acagggcttc gtgcttttgt gcaaacaggt gagtatcggt aatttgtaaa atcctgactc
    34861 tggcctcacc agccagagga agggttaaca ggctttccaa atggtgtcct taggtttcac
    34921 gacgctagta aaccggaatc gccatcgctc catgtgctaa acagtatcgc aaccagtccg
    34981 ctaaaaactg gttaatttga tgcttttcgt cgcgttgatg caacttttta ttaggataat
    35041 cataccgcgc tttgaagcga aaaatctgct ggcttgaaca cacttcagcc accatcgcgt
    35101 catgatacag acgcaccgtc attgacggaa ggctccagta actgatcgcg ggcgcagtct
    35161 gttctattgt caccagggta gtgtatcggg tcgattccac aatcgtcagc cgatattgtg
    35221 cgtttgccac ctgatagctt acagtttcgc cgggtgcgtc attgcgcggt aacaaacggc
    35281 gcaattgtga aaagttcatc tcgcacaggc gcatcatttc aggaaagtca ggtgtgtaac
    35341 gcttcattta tgcccactca ttttttaacg cttgatgatg cagctgcagc cattgcaaag
    35401 cgatgaccga cgctgcgttg tcgattttcc cctcttctac ccactggtat gcctgttccc
    35461 ggcttaccac atgaacgcga atatcttcgt tttcatcagc cagaccatga ataccgcttg
    35521 cggtcgtggc gtccacttcg cccaccataa ttgacgaacg ctcactggtg ccccccgggc
    35581 ttgccaggaa acttaacacc ggtttggtcc gtttgactat cagtcccgcc tcttcaatcg
    35641 cttcgcgacg ggcaacatct tccacacttt caccctcttc tatcatcccg gcaaccatct
    35701 ccagtagcca gggggtttcg ctggtgtcgt acgcggcaat ccgaatctgc tcaatcagca
    35761 caacttcatc acgcactggg tcaaagggta gcaagactgc ggcgtgaccg cgctcaaaaa
    35821 tttcccgccg tacctcatga ctcatttgcc cgttgaatag acgatgacga aatctataaa
    35881 gatctaatga aaaaaagccg cgataaagtg tttctcgtgc aataatttct acatcgtttt
    35941 tgccaaatgt aacgggcagg ttgtctggct taagcattgt taatgtcctg gcactaatag
    36001 tgaattaaat gtgaatttca gcgacgtttg actgccgttt gagcagtcat gtgttaaatt
    36061 gaggcacatt aacgccctat ggcacgtaac gccaaccttt tgcggtagcg gattctgcta
    36121 gaatccgcaa taattttaca gtttgatcgc gctaaatact gcttcaccac aaggaatgca
    36181 aatgaagaaa ttgctcccca ttcttatcgg cctgagcctt tctgggttca gttcgttgag
    36241 ccaggccgag aacctgatgc aagtttatca gcaagcacgc cttagtaacc cggaattgcg
    36301 taagtctgcc gccgatcgtg atgctgcctt tgaaaaaatt aatgaagcgc gcagtccatt
    36361 actgccacag ctaggtttag gtgcagatta cacctatagc aacggctacc gcgacgcgaa
    36421 cggcatcaac tctaacgcga ccagtgcgtc cctgcagtta actcaatcca tttttgatat
    36481 gtcgaaatgg cgtgcgttaa cgctgcagga aaaagcagca gggattcagg acgtcacata
    36541 tcagaccgat cagcaaacct tgatcctcaa caccgcgacc gcttatttca acgtgttgaa
    36601 tgctattgac gttctttcct atacacaggc acaaaaagaa gcgatctacc gtcaattaga
    36661 tcaaaccacc caacgtttta acgtgggcct ggtagcgata accgacgtgc agaacgcccg
    36721 cgcgcagtac gataccgtgc tggcgaacga agtgaccgca cgcaataacc ttgataacgc
    36781 ggtagagcag ctgcgccaga tcaccggtaa ctactatcca gaactggcag cgctgaatgt
    36841 cgaaaacttt aaaaccgaca aaccacagcc ggttaacgcg ctgctgaaag aagccgaaaa
    36901 acgcaacctg tcgctgttac aggcacgctt gagccaggac ctggcgcgcg agcaaattcg
    36961 ccaggcgcag gatggtcact taccgacgct ggatttaacg gcttctaccg ggatttctga
    37021 cacctcttat agcggttcga aaacccgtgg tgccgctggt acccagtatg acgacagcaa
    37081 tatgggccag aacaaagtgg gcctgagctt ctcgctgccg atttatcagg gcggaatggt
    37141 taactcgcag gtgaaacagg cacagtacaa ctttgtcggt gccagcgagc aactggaaag
    37201 cgcgcatcgt agcgtcgtgc aaaccgtacg ttcctccttc aacaacatta atgcatctat
    37261 cagtagcatt aacgcctaca aacaagccgt agtttccgct caaagctcat tagacgcgat
    37321 ggaagcgggc tactcggtcg gtacgcgtac cattgttgat gtgttggatg caaccaccac
    37381 gctgtacaac gctaagcaag agctggcaaa tgcgcgttat aactacctga ttaatcagct
    37441 gaatattaag tcagccctgg gtacgttgaa cgagcaggat ctgctggcac tgaacaatgc
    37501 gctgagcaaa ccggtttcca ctaatccgga aaacgttgcc ccgcaaacgc cggaacagaa
    37561 tgctattgct gatggttatg cgcctgatag cccggcaccc gtcgttcagc aaacatccgc
    37621 acgcactacc accagtaacg gtcataaccc tttccgtaac tgatgacgac gacggggctt
    37681 cggccccgtc tgaacgtaag gcaacgtaaa gatacgggtt atctgccgca ttcttccccc
    37741 ttctcgcttc aatttcgacc agccatcctc tattctgatg ggtatttacc actggtcccg
    37801 gaagacaaaa atgaaacgga caaaatccat acgccacgca tcgttccgca aaaactggag
    37861 cgcgcgccat ctgacaccag tcgctctcgc ggttgccact gtttttatgc tggctggctg
    37921 tgaaaagagt gatgaaacag tgtctctcta tcaaaatgct gacgactgtt cagctgcaaa
    37981 cccaggcaaa agcgccgaat gtaccaccgc gtacaacaat gcgctgaaag aagccgaacg
    38041 tactgcgccg aaatacgcca cccgtgaaga ctgtgttgct gaatttggtg aaggtcagtg
    38101 ccagcaggca ccagcccagg ctggcatggc accagaaaac caggcgcagg cccagcaatc
    38161 cagcgggagt ttctggatgc cgctgatggc cggttacatg atggggcgtc tgatgggcgg
    38221 cggcgcggga tttgcacagc agccgctgtt ctcctcgaaa aacccagcca gcccggctta
    38281 cggtaaatat accgacgcga cgggtaaaaa ctatggcgca gcccagccag gccgcaccat
    38341 gaccgtaccg aagacagcca tggcaccaaa accggcgacc accactaccg ttacccgtgg
    38401 cggttttggt gaatctgttg ccaaacaaag cactatgcag cgtagcgcaa ctggcacctc
    38461 ttctcgttca atgggtggct gataccgatg gaaagagtca gtattaccga gcgcccggac
    38521 tggcgtgaga aagcccacga atacggtttc aattttcaca ccatgtacgg cgagccgtac
    38581 tggtgtgaag atgcttacta caagttgacc ctcgcccagg ttgaaaagct ggaagaagtc
    38641 accgccgaac tgcaccagat gtgcctgaaa gtggtggaaa aagtgatcgc cagcgatgag
    38701 ctgatgacca aattccgcat tccaaaacac acctggagtt ttgtgcgcca gtcatggctg
    38761 acgcaccagc catcgcttta ttcgcgtctt gatctggcgt gggatggcac tggcgaacct
    38821 aaacttctgg aaaataacgc cgatacgcca acgtcactat acgaggcggc gttctttcag
    38881 tggatctggc tggaagatca gcttaacgcc ggtaacttgc cggagggcag cgaccagttt
    38941 aacagtctgc aagaaaaact gatcgatcgc ttcgttgagc tgcgtgaaca gtatggcttc
    39001 cagttgctgc atctcacctg ctgtcgcgac acggtggaag atcgcggaac cattcagtat
    39061 ttgcaggact gcgcaacgga agctgaaatt gctactgagt tcctctacat cgatgatatc
    39121 gggttaggtg aaaaaggtca gttcacggat ttacaggatc aggtaatttc caacctgttc
    39181 aagctgtatc cgtgggaatt tatgttgcgt gagatgtttt ccaccaagct ggaggatgca
    39241 ggcgtacgct ggctggaacc ggcgtggaag agcattatct ccaacaaggc acttctaccg
    39301 ctactgtggg agatgttccc gaatcacccg aacctgctgc ccgcttattt tgcggaagat
    39361 gatcatccgc aaatggaaaa atatgtggtt aaaccgatct tctcccgtga aggcgcaaac
    39421 gtgtcgatca ttgaaaacgg caaaaccatt gaagcagcgg aaggtccgta tggcgaagaa
    39481 gggatgattg ttcagcaatt ccacccgtta ccgaaattcg gcgacagcta tatgctgatt
    39541 ggtagctggc tggtgaacga tcaacccgcc ggaattggca ttcgtgaaga ccgtgcattg
    39601 atcacccagg atatgtctcg gttttatcca catatttttg ttgagtaatc aatgatgccg
    39661 gatggcattc gccatccggt aattgttaac ctatctgcac cgacagcata ctcaggctgc
    39721 ccatttctat accatcgacc ggaatggtaa ttggctcctg cccatcccac gcacctaaga
    39781 catacaacaa cggcagatag tgctccggcg ttgggttcga taacgcgcca ccttcatggt
    39841 cgaggtaatt caccagagga tgctgctcta ccgccccttg ccacgtcaga ttcgctttca
    39901 catactcatt aaacgacatc gcccaaggat atggtgaact atcaccgtgc cacttcactg
    39961 tacgcaggtt atgcaccacg ttgccactgg cgaccaacat tattccttca tctcgcagcg
    40021 ctgccagttt gcgccccatt tcgaaatgcc aggcggcagg tttgctactg tcgatactca
    40081 actgcaccat cgggatatcg gcgtcaggat acatcttaat cagcacgccc caggaaccgt
    40141 ggtcaaagcc ccaggcttct ttatccagcg tcaccgggac cggtgctaac agctcaacca
    40201 gatgctgtgc cagcacaggc gaacccggag caggataatg cgtatcgtac agcgcctgcg
    40261 ggaagccacc aaagtcatga atcgtgggcg gcgtctccat cgcggtcact cctgttccac
    40321 gggtaaacca gtgagccgaa accaccacaa tcgcttgcgg gcgtggcaat gtcatcccca
    40381 acttctgcca gctgcgggta tacaaattat cttccagcac gttcatcgga ctaccgtgac
    40441 ctaaaaacaa tgctggcata cgtgttgaag acatgatgat atccttaact aaaggtgtca
    40501 ttttgatatc ctcacaatac gcttgttcgg cggagtaaga acccggataa caatgatgat
    40561 gatcatcagt tattttgacg atctgcctga aggtgaagat ttataaggag ttgtcgatgt
    40621 cagtacctct cattctgacc atactggcgg gggcagccac gtttattggc gcgtttctcg
    40681 gcgttctcgg gcaaaaaccc tcgaaccgct tactggcgtt ttcgctagga tttgcggcgg
    40741 ggatcatgtt gctcatctca ttaatggaaa tgcttcctgc cgcactagca gctgaaggaa
    40801 tgtcgcctgt gttgggttat ggaatgttta tcttcggtct gcttggctat tttggcctgg
    40861 accgcatgtt gccacatgcg catccgcagg atttaatgca aaaatcggtg cagccgttgc
    40921 caaaatcgat caagcgcaca gccattctgc tcactctcgg catcagtctg cataacttcc
    40981 cggaagggat tgccaccttt gtcacggcga gcagcaacct ggagctggga tttggcatcg
    41041 cactggccgt cgcgttgcac aatattcctg aaggtctggc ggttgcaggc ccggtttatg
    41101 cagcaacggg ttctaaacgc acggcaattc tgtgggcggg gatttctgga ttggcagaaa
    41161 ttcttggtgg tgtgctggcg tggttaatcc tcggtagcat gatttccccc gtggtcatgg
    41221 cggcaatcat ggcggcggtt gcaggaatta tggtggcgct ctcggttgat gaattaatgc
    41281 cgctcgccaa agagattgac cctaataata accccagcta tggcgtacta tgtggaatgt
    41341 cagtgatggg attcagttta gtgctgctac aaacggcggg aattggttaa aagcaacatc
    41401 agaagttggc cagcgcatgg acaacttctg ctagcttcac taaagcctta ttctaccttt
    41461 tcggctgtct cttctctcgt actgtttaag tatttgttcc gcgcttcaag cagtgcgtct
    41521 ctgtcctggc gaaacgcccg acaataacgt ttcaaatgca ggaaataacc caccacaaca
    41581 agagcaatca atacaatcca gtaccaggcg ataaacatcc ttttaccctt aatatttaac
    41641 aatactaaac atttagcgca taaatttcac atatcctttt tcggatatat tcatatggtc
    41701 gcataataac ggaacaatta tatgattttg atcataatga aaaagaatgc ctgatattaa
    41761 tgaaattttc tcatagaaag agaagcggaa ttaacatctt gcatagcagg gcaaaataaa
    41821 aaccgaagcc agctacaact tcggtttctt gattaaggca gtaattaagc agcggttttc
    41881 agctggcttt acgctcatgt gcctgacggt atgccaccag gtcttcaata gtcacgagcg
    41941 ccatattgtg tttattggca aactcaatgc actctggtgc acgcgccatc gtgccatcgt
    42001 cattagtcaa ctcacacagc acaccagccg gtttaaagcc tgccagcgtc atcaaatcaa
    42061 tagtcgcttc agtatgaccg ccacgcgtca gtacaccacc tgcctgcgcg cgaagtggga
    42121 aaacgtggcc cgggcgattc agatcagaag gttttgcgcc atcggcaatc gctgcgcgaa
    42181 cggtcgtaat tcgatcagcg gcagaaacac cggtagtcac accttcagcc gcttcaatgg
    42241 tcacggtaaa accggtgcca taggcgctgg tgttattttc taccatcatt ggcagatcaa
    42301 gttgtttacg gcgatcttca gtgatgcaca ggcaaacaat accgctaccg tggcgaatgg
    42361 tcagcgccat ctgctcaacg gtcatggttt ctgccgggaa gatcatatcg ccttcgtttt
    42421 cacggtcttc atcatcaagc accattacac cgcgtccttc acgcagcgca gccagtgcat
    42481 tttcaacacg ttcgaaaggc gtaccaaaag aggaaagtag cgtctgattc atggtaaaaa
    42541 aacctcacta aaattatggt taccagaatc agggcagtct taggagtggc ggcatatagc
    42601 caaaataacg tgagcgggtc catgcccgac agaatcgtta ctctctccca tccggactct
    42661 aaccgtcggc cccggaatta caccggatct gctgtccttt gagttcgcac ccaaagcgct
    42721 cgcgggcttt caactgagtt gatttaccgc cggtggggaa tttcgccccg ccctgagaat
    42781 aagcggattc actataacgc taatgattag cggcagcaac gcatagcttc acataattct
    42841 ggtttatgac ttacccttat cgcactacaa tggcactcaa cacctatcag tacagggaaa
    42901 ctacaatgat tgacccgaaa aaaattgagc aaatcgctcg ccaggttcac gaatcaatgc
    42961 ctaaaggaat cagggagttc ggggaagatg tggagaaaaa aatccgccaa accctacaag
    43021 ctcagctgac gcgcctcgac cttgtaagcc gtgaagagtt cgacgtccaa acgcaagtcc
    43081 tgttacggac tcgtgagaaa ctggcgctgc ttgaacaaag aatgagcgaa ctggaaaatc
    43141 gcagtactga gattaaaaag cagccagatc cagagactct ccccccaacg ctgtaaacat
    43201 ttgtctttaa tgcactgtaa attatgaatt aatc
//
LOCUS       DAZGJW010000021        42720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZGJW010000021 DAZGJW010000000
VERSION     DAZGJW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.021"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..116
                     /locus_tag="ACHKIK_001231"
     CDS             <1..116
                     /locus_tag="ACHKIK_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9082444.1"
                     /translation="IKLEHPLPSIKLYISYNKSSLNNLVFSRFIDRLNESF"
     gene            263..1483
                     /gene="ybdN"
                     /locus_tag="ACHKIK_001232"
     CDS             263..1483
                     /gene="ybdN"
                     /locus_tag="ACHKIK_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO9082445.1"
                     /translation="MSVYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1456..2085
                     /gene="ybdM"
                     /locus_tag="ACHKIK_001233"
     CDS             1456..2085
                     /gene="ybdM"
                     /locus_tag="ACHKIK_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO9082446.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2086..3246)
                     /gene="ybdL"
                     /locus_tag="ACHKIK_001234"
     CDS             complement(2086..3246)
                     /gene="ybdL"
                     /locus_tag="ACHKIK_001234"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO9082447.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3355..4443
                     /gene="hcxA"
                     /locus_tag="ACHKIK_001235"
     CDS             3355..4443
                     /gene="hcxA"
                     /locus_tag="ACHKIK_001235"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO9082448.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4453..4650)
                     /gene="ybdD"
                     /locus_tag="ACHKIK_001236"
     CDS             complement(4453..4650)
                     /gene="ybdD"
                     /locus_tag="ACHKIK_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO9082449.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4832..6937)
                     /gene="cstA"
                     /locus_tag="ACHKIK_001237"
     CDS             complement(4832..6937)
                     /gene="cstA"
                     /locus_tag="ACHKIK_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO9082450.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7118..7531)
                     /gene="entH"
                     /locus_tag="ACHKIK_001238"
     CDS             complement(7118..7531)
                     /gene="entH"
                     /locus_tag="ACHKIK_001238"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO9082451.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7534..8280)
                     /gene="entA"
                     /locus_tag="ACHKIK_001239"
     CDS             complement(7534..8280)
                     /gene="entA"
                     /locus_tag="ACHKIK_001239"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO9082452.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8280..9137)
                     /gene="entB"
                     /locus_tag="ACHKIK_001240"
     CDS             complement(8280..9137)
                     /gene="entB"
                     /locus_tag="ACHKIK_001240"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO9082453.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9151..10761)
                     /gene="entE"
                     /locus_tag="ACHKIK_001241"
     CDS             complement(9151..10761)
                     /gene="entE"
                     /locus_tag="ACHKIK_001241"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO9082454.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10771..11946)
                     /gene="entC"
                     /locus_tag="ACHKIK_001242"
     CDS             complement(10771..11946)
                     /gene="entC"
                     /locus_tag="ACHKIK_001242"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO9082455.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12315..13271
                     /gene="fepB"
                     /locus_tag="ACHKIK_001243"
     CDS             12315..13271
                     /gene="fepB"
                     /locus_tag="ACHKIK_001243"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO9082456.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(13275..14525)
                     /gene="entS"
                     /locus_tag="ACHKIK_001244"
     CDS             complement(13275..14525)
                     /gene="entS"
                     /locus_tag="ACHKIK_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO9082457.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14636..15640
                     /gene="fepD"
                     /locus_tag="ACHKIK_001245"
     CDS             14636..15640
                     /gene="fepD"
                     /locus_tag="ACHKIK_001245"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO9082458.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15637..16629
                     /gene="fepG"
                     /locus_tag="ACHKIK_001246"
     CDS             15637..16629
                     /gene="fepG"
                     /locus_tag="ACHKIK_001246"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO9082459.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16626..17441
                     /gene="fepC"
                     /locus_tag="ACHKIK_001247"
     CDS             16626..17441
                     /gene="fepC"
                     /locus_tag="ACHKIK_001247"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO9082460.1"
                     /translation="MNESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17438..18571)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKIK_001248"
     CDS             complement(17438..18571)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKIK_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO9082461.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18787..22668)
                     /gene="entF"
                     /locus_tag="ACHKIK_001249"
     CDS             complement(18787..22668)
                     /gene="entF"
                     /locus_tag="ACHKIK_001249"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO9082462.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22665..22883)
                     /gene="ybdZ"
                     /locus_tag="ACHKIK_001250"
     CDS             complement(22665..22883)
                     /gene="ybdZ"
                     /locus_tag="ACHKIK_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO9082463.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22886..24087)
                     /gene="fes"
                     /locus_tag="ACHKIK_001251"
                     /pseudo
     CDS             complement(22886..24087)
                     /gene="fes"
                     /locus_tag="ACHKIK_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
     gene            24330..26570
                     /gene="fepA"
                     /locus_tag="ACHKIK_001252"
     CDS             24330..26570
                     /gene="fepA"
                     /locus_tag="ACHKIK_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO9082464.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDILVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGMSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26610..26708
                     /locus_tag="ACHKIK_001253"
                     /pseudo
     CDS             26610..26708
                     /locus_tag="ACHKIK_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26745..27365
                     /gene="entD"
                     /locus_tag="ACHKIK_001254"
     CDS             26745..27365
                     /gene="entD"
                     /locus_tag="ACHKIK_001254"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO9082465.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27487..27639)
                     /gene="hokE"
                     /locus_tag="ACHKIK_001255"
     CDS             complement(27487..27639)
                     /gene="hokE"
                     /locus_tag="ACHKIK_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO9082466.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(27990..28142)
                     /gene="hokE"
                     /locus_tag="ACHKIK_001256"
     CDS             complement(27990..28142)
                     /gene="hokE"
                     /locus_tag="ACHKIK_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO9082467.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            28111..28380
                     /locus_tag="ACHKIK_001257"
     CDS             28111..28380
                     /locus_tag="ACHKIK_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9082468.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            28584..29702
                     /gene="ybdK"
                     /locus_tag="ACHKIK_001258"
     CDS             28584..29702
                     /gene="ybdK"
                     /locus_tag="ACHKIK_001258"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO9082469.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            29768..30016
                     /gene="ybdJ"
                     /locus_tag="ACHKIK_001259"
     CDS             29768..30016
                     /gene="ybdJ"
                     /locus_tag="ACHKIK_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO9082470.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWQRWFSLAD"
     gene            30081..30449
                     /gene="ybdF"
                     /locus_tag="ACHKIK_001260"
     CDS             30081..30449
                     /gene="ybdF"
                     /locus_tag="ACHKIK_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO9082471.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30543..31196
                     /gene="nfsB"
                     /locus_tag="ACHKIK_001261"
     CDS             30543..31196
                     /gene="nfsB"
                     /locus_tag="ACHKIK_001261"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO9082472.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            31304..32551
                     /gene="ybdG"
                     /locus_tag="ACHKIK_001262"
     CDS             31304..32551
                     /gene="ybdG"
                     /locus_tag="ACHKIK_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO9082473.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(32619..33995)
                     /gene="pheP"
                     /locus_tag="ACHKIK_001263"
     CDS             complement(32619..33995)
                     /gene="pheP"
                     /locus_tag="ACHKIK_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO9082474.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(34097..37240)
                     /gene="cusA"
                     /locus_tag="ACHKIK_001264"
     CDS             complement(34097..37240)
                     /gene="cusA"
                     /locus_tag="ACHKIK_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO9082475.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(37252..38475)
                     /gene="cusB"
                     /locus_tag="ACHKIK_001265"
     CDS             complement(37252..38475)
                     /gene="cusB"
                     /locus_tag="ACHKIK_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO9082476.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(38491..38823)
                     /gene="cusF"
                     /locus_tag="ACHKIK_001266"
     CDS             complement(38491..38823)
                     /gene="cusF"
                     /locus_tag="ACHKIK_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO9082477.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(38981..40354)
                     /gene="cusC"
                     /locus_tag="ACHKIK_001267"
     CDS             complement(38981..40354)
                     /gene="cusC"
                     /locus_tag="ACHKIK_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO9082478.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQETRAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSIRLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGG"
     gene            40511..41194
                     /gene="cusR"
                     /locus_tag="ACHKIK_001268"
     CDS             40511..41194
                     /gene="cusR"
                     /locus_tag="ACHKIK_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO9082479.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            41184..42632
                     /gene="cusS"
                     /locus_tag="ACHKIK_001269"
     CDS             41184..42632
                     /gene="cusS"
                     /locus_tag="ACHKIK_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO9082480.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT        10221 a        11818 c        10912 g         9769 t
ORIGIN      
        1 agataaaact agaacatccg ttaccttcaa taaagcttta catttcctat aataaatctt
       61 ctttaaataa tttggtgttc tcccgattta ttgaccgtct aaatgagagt ttttgatata
      121 taactacaga cagctactat aactttcatc tatttattca cagcgcgaat gaaaaataaa
      181 acactctgct ttgcagtgtt tactttataa ttaacagact tatctcactg atcaccctgt
      241 aacgttcaga gagcgtcttc agatgtctgt ttataaaatt cctcttccgc tcaatattct
      301 ggaagctgca cgagagcgta ttacctggac actcaacacc ctaccccgcg tttgtgtctc
      361 attttctggc ggcaaagact ctggtttaat gctgcatctg acagccgaac ttgcccgaca
      421 aatgggcaaa aaaatctgcg ttttgtttat cgactgggag gcgcaattct cttgcaccat
      481 taactatgtt cagtccctgc gcgagttgta caccgatgtc atcgaagagt tttactgggt
      541 tgcgctcccg cttacgacgc aaaattccct ttcacaatac caacccgaat ggcagtgctg
      601 ggaacctgat gtcgaatggg tgcgtcagcc cccgcaagat gcgataaccg atcctgactt
      661 tttctgcttt taccagccag gcatgacctt cgaacaattt gtacgtgagt ttgccgaatg
      721 gttttcacaa aaacgtccgg cggcgatgat gatcggcatc cgtgcggatg agtcctacaa
      781 ccgttttgtc gccatcgcca gtttaaataa acaacgtttt gccgacgata aaccatggac
      841 tacggccgca ccaggcggtc atagctggta catttacccc atttacgact ggaaagtggc
      901 tgatatctgg acctggtatg caaatcatca aaatctctgc aacccactgt ataacctaat
      961 gtatcaggca ggcgttcctc tgcgtcatat gcgaatttgc gaaccttttg gcccggagca
     1021 acgacaagga ttatggctct atcacgttat cgaaccggat cgctgggctg ctatgtgcgc
     1081 acgagtcagc ggggtaaaaa gtggcggcat ttacgccgga catgataatc atttctatgg
     1141 gcatcggaaa atcctcaagc cagaacattt agactggcaa gaatatgcat tattgctgct
     1201 caatagcatg ccggaaaaaa cagctgagca ttaccgcaat aaaatcgcca tttatttgca
     1261 ctggtatcag aaaaaaggca tcgaggttcc acaaacccag caaggggaca ttggcgcgaa
     1321 agatatcccc tcctggcggc ggatatgcaa agttttactc aataacgatt actggtgtcg
     1381 ggcattatca tttagtccta cgaaagcgaa gaactatcag cgttataacg aacggataaa
     1441 aggaaaacgt caggaatggg ggatactatg caacaacgat taacgcagga tttaacgcaa
     1501 tttctcgcca gcttgccgga agatgatcgc atcaaagcga ttaatgaaat ccgcatggcg
     1561 atccatcagg ttagcccctt tcgtgaagag ccagtggatt gcgttctgtg ggttaaaaac
     1621 agccaactta tgcctaatga ttacaaccca aacaacgtgg caccacccga aaaaaaactg
     1681 ctgaaaaaat ccattgaaat agatggtttt acccaaccca ttgtggtcac gcatacggat
     1741 aaaaatgccc tggaaatcgt ggatggtttt caccgccatg aaattggtaa aggctcatca
     1801 tcattaaaat tacgcttaaa aggttatttg ccggtgacct gtctggaagg tacgcgtaat
     1861 caacgcatcg ccgcgactat tcgtcataat cgcgctcgtg gacgccatca aatcaccgcc
     1921 atgtcggaaa tcgtccgtga acttagccag ttgggatggg acgataataa aattggcaaa
     1981 gagctaggaa tggacagcga cgaagtgctg cgcctgaagc aaattaacgg cctgcaagag
     2041 ctgtttgccg accgtcaata ttcccgcgcc tggacggtta aataactaaa gctggcgcag
     2101 gcgttcagct gctgccagca acgtcgattc cttcttggca aaacagagac gaatcagttt
     2161 atgtgggaag ggatcggcgc aaaacaccga cagcggaatc gccgctacgc cgtgctcctg
     2221 cgtcagccac tggcaaaact caacatcatc cagggtagaa acctcgctgt aatccaccag
     2281 caaaaagtat gtaccttcac acggtaaaat ctccagccgg ctttcattta aagcattcac
     2341 cagaatatcg cgcttctggc gataaaagtc cggtaacgca agataatgct caggttctgc
     2401 acgtagcata tcagcaagcg ccagctgtgc cggggtattc accgaaaagg tcagatactg
     2461 atgtaccttg cgaatttcgg cgctgatggg cgctggcgca acacaataac ccactttcca
     2521 gccggtcata tgataggtct tgccaaatga agaaaccgcc actgcccgct cacgcagctg
     2581 cggatgcgcc agcacactgg catggccctg ttgtgaaaag ttgatgtgct cgtagacttc
     2641 atcgctaatg acaaaaatct cgtgcccggc gatcgcctgc cacaaagcgg cgaaatcagc
     2701 ctgctgccag acagttgcac tggggttatg cggagtgttg aggatcacca gtctggtgcg
     2761 ctcgcttaat aatgcggcaa attcctgcca gtcaacgcga aaatgcggtg gttgcagtgc
     2821 catacgcttc actattcccc cagaaagcgc gatggcgggg gagtaactgt catagctggg
     2881 atcaaaacaa atcacttcat cgccattgcg caccagtgcg gtaatcgccg cgtataacgc
     2941 ttccgtcgcc cctgccgtta cggtgatatc gctatcggca tctggttgat agccatacaa
     3001 acgttccgtt ttctgagcaa tcgcctcgcg caaggcctgc acgccggtca tgggcgcgta
     3061 ttggtttgcc ccctgggcaa cgtggtacgc cagccgctcc tgtaaatagc gcggaccatc
     3121 aaaatcagga aagccttgcg acaggttaat cgcctggtgt tgctgcgcca gcgcgctcat
     3181 ctgggtgaaa atagtggtgc caagttgtgg aagtttgctt tgtggaatca gagggttatt
     3241 tgtcattttt attgtacctg acgaagttgc gtgttgaagg cactataaca cgatgctagt
     3301 atttggcaat caagacgttt agatgtctaa ataaaacaat aaggacaaca caacatgcct
     3361 cacaatccta tccgcgtggt cgtcggcccg gctaactact tttcacatcc aggaagtttc
     3421 aatcacctgc acgatttttt cactgatgaa caactttctc gcgcggtgtg gatctacggc
     3481 aaacgcgcca ttgctgcggc gcaaaccaaa cttccgccag cgtttggact tccaggggca
     3541 aagcatattt tgtttcgtgg tcattgcagc gaaagcgatg tacagcaact ggcggctgag
     3601 tccggtgacg atcgcagcgt ggtgattggc gtcggcggcg gcgcactgct cgacaccgcg
     3661 aaagccctcg cccgccgtct cggtctgccg tttgttgccg ttccgacgat cgccgccacc
     3721 tgcgccgcct ggacaccgct ctccgtctgg tataacgatg ccggacaggc gctgcattat
     3781 gagattttcg acgacgccaa ttttatggtg ctggtggaac cggagattat cctcaatgca
     3841 ccgcaacaat atctgctggc ggggatcggt gacacgctgg cgaaatggta tgaagcggtg
     3901 gtgctggctc cgcaaccaga aacgttgccg ctaaccgtgc gactggggat caataatgcg
     3961 caggccattc gcgacgtatt gttaaacagt agcgaacagg cgctgagcga tcagcaaaat
     4021 caacagttaa cgcaatcatt ttgcgatgtg gtggatgcta ttattgctgg tggtgggatg
     4081 gtcggtggtc tgggcgatcg ttttacgcgt gtggcggcgg ctcatgccgt gcataacggt
     4141 ctgactgtcc tgccgcaaac cgagaaattt ctccacggca ccaaagtcgc ttatggcatt
     4201 ctggtacaaa gcgccttgct gggtcaggat gatgtgctgg cgcaattaac cggggcgtat
     4261 cagcgttttc atctgccgac cacgctggcg gagctggaag tggatatcaa taatcatgcg
     4321 gagatcgaca aagtgattac ccacaccctg cgtccggtgg aatccattca ttacctgcca
     4381 gtcacgctga caccagatac gttgcgtgca gcgttcgaaa aagtggaatc gtttaaagcc
     4441 tgacgtgcaa atttagcagc agcgcgcgcc gccttttcca ccgtagcgcg cgtcctgccg
     4501 ctcacggaaa aactcttcgt aggtcatcgg cgtttgatcg ggatggttaa cccgcatatg
     4561 ttcgacatag ttgtcgtaat caggcatacc aatcatcagc ttcgccgcct gacctaaata
     4621 ttttccggct tttgccagtg aatcaaacat cttggcctcc gctaaccatg ttgctgaaag
     4681 agtgccggat gcggcgtgaa cgccttatcc ggcctacaaa tgctatcaac gcgctcggtc
     4741 agtcccctcg ccccatcggg gagagggtta gggtgaggag gaaccctcgg cactatgcca
     4801 acattcgcca acatcccccc tcactctgac tttagtgtgc gccttttgcc tgtgccacga
     4861 tctcctcgac attttccggc attggctcat acggcgtttc tttcgccgtt ggcttcggat
     4921 ctttcaatgc cgccagtgcc gtcttaatcg agaacagcgc cagaaccacc acgaccacca
     4981 taaagaagat ggttaacccg gcatccagac ggttgttgaa caccagttgc gccagctgtg
     5041 actcagtata ctgcgacgga atattgccgc tgtcgatcat tgcctggaac ttattagcaa
     5101 tggccaggaa gccgactttc gcatccgggc taaacgcttt ctgccagcct gcggtcaggg
     5161 tacaaatcag cagccaggcc gttggtacca gcgccaccca ggcgtaacgt tgacgtttca
     5221 tcttgaacaa caccacggca cagagcatca gcgccatccc tgccagcatc tggttggcaa
     5281 taccaaacag cggccacaga gtgttaatgc cgcccagcgg atcgaccacg ccctgatgga
     5341 ggaagtagcc ccacgccagt acgcacagcg ctgttgccag caggttagca ggcagtgaat
     5401 cggtccgctt caggcccgga gaaaccacgc ccagcagatc ctgcaacata aagcgcgcag
     5461 cacgcgtacc cgcatctacc gccgtcagaa taaacagagc ttcaaacaga atggcgaagt
     5521 gataccagaa cgccacatcc atcataccac ccagcgcacc gtgcaggatg taagccatcc
     5581 ccaccgccag cgtcggcgca ccgcccgcac gggaaatgat cgactgttca cccacttcgc
     5641 tggcaatctg gttgagcgta tctggtgtaa tgctaaagcc ccagctactc accacctgcg
     5701 cggcagaagc gactacatcc gccgtccctg ccggagccag caccgccatt gggctgttca
     5761 tggcaaaata cacgcccgga tcgatgatac aggcagaaac cagcgccata atcgccacga
     5821 aggattccat caacatccca ccgtagccga taaagcacgc ctgcccttcg ttcgccagca
     5881 tcttcggcgt ggtgccggaa gagatcagcg catggaagcc agacaccgca ccacaggcga
     5941 tggtgataaa caggaacggg aacaggttac cggtccatac cgggccagtg ccatcgacaa
     6001 atttggtcag cgcaggcatg gtcagcgtcg ggcgcataat caaaatgcct accgccagac
     6061 cgacgatagt cccgattttc aggaaggtcg agagatagtc acgcggggcc agcagcaacc
     6121 acaccggcag caccgccgcc acaaaaccgt agcccaccag catccaggtc agctgcacgc
     6181 cggtaaagtc aaagtacggt gcccaggtcg gactttctgc cacccagccg ccagagataa
     6241 tggcgaaaat caggaacacc aggccaatca ctgacacttc gccaatgcgg cccggacgca
     6301 gatagcgcag gtagattccc ataaaaatcg ccagcggaat ggtgaacgca acggtatacg
     6361 ttccccacgg gctatgagtc agggctttca ccacgatcat cgccagtact gcaaggataa
     6421 tgaccatgat cataaagcag gctaccagcg caatcacccc ggcagttggc cccatctctt
     6481 ctttgaccag ctcacccagc gatcgaccgt cacggcgcgt agaaacaaac agcaccatga
     6541 aatcctgcac cgcaccagcg agaaccaccc cggcgagcag ccagatcatc cctggcaggt
     6601 aacccatttg cgccgccagt accggcccaa ccaacggacc tgctccggca atggccgcaa
     6661 aatggtgacc gaacagcact ttcttgtccg tcggcacata gtccagcccg tcgttatggc
     6721 gcaccgctgg cgtcatacgc gtcgggtcaa ccgccagcac atttttggcg atatacagcc
     6781 cataaaaacg gtaagcgatc agatagatac agaccgacgc caccacaatc cacagcgcgt
     6841 tgatctgttc cccacgattt aaagcaatgt atcccagagc aaatgctccc attacagaga
     6901 gcactgtcca gacgaggtat ttccctgatt tgttcatagt tgttatccgt gtgcgtgtcc
     6961 atagagatgt tacattttgt ttctataaca tctttgtaag atttaacaac acggtaacca
     7021 tgtaaatcgg agttgaaacc aatatttaac ttaacaatgt taactcgatc actccgttaa
     7081 ccgagttttc ctgcaatctc atttaattct gtcgggctca tcccaaaact gccgtaccca
     7141 gccgacaagt gcagcaacgc cgcccctgtt catcgaaaac gacgatttcc cagctctgat
     7201 tttgccgccc aagatgcagc ggctggcaga cgccgcgtac cttgccttca gacaccgggc
     7261 gatgatgggt ggcgttaagt tccgtaccca ccacacactg cccgtcacgg gtcatcataa
     7321 atccggccat cgatcccagc gtttcagcca gcgccgccga cgcgccgcca tgcagcaggc
     7381 caaacggctg atgagtacgg gtatcaaccg gcatttcggc ttccagcaca tcatctccca
     7441 gacgggtata cacaattccc agatgcgcca ccattgtgtt atcgctggtg gcgttcagtt
     7501 cgtcgagcgt taaatggcgt ttccagatca tgcttatgcc cccagcgttg agccgccatc
     7561 gaccacaata tcctgtaggg tgatatggct ggcgaggtca gaggcgagga acaaaatcgt
     7621 gttggcgatc tcttgtggac gggcgatttt ccccagcgga atgccgagtt taaactgctc
     7681 gccaaagccg cgaatacgct gttcttcggc gtcatcgctc acccacagcg tgcgttgcat
     7741 atcggtgtcg gtggagccag gcgaaaccac attacagcgc acgccgctac ccgccagttc
     7801 cagcccgacg ctcaacgcca ggcttttcag cgccgctttc gatgcgccat aagcactcat
     7861 accaatacgc ggcgtgtgtg cggcgtcgga cgccacagtg acaatcgccc cgccccgctg
     7921 acggcgaaac tggttcatgg tttgctggaa caggttaaac gcaccgccga cgttaaccgc
     7981 aaaagtctgc tgccagtcct ctttactgag ctgatcggtc gcgcccatgc gcaaaattcc
     8041 cgccgcattg atcagcacgt ccagtcgctc cgtttcagct aacagtcgct gacatacctg
     8101 cgcgacctgc gcagcatcgg caacatccat cacttcggtc gcaaagggat attgctcctg
     8161 agcgaacgct tgatcaaaac cggtaacttt cgctcccgcc tcaacaaacg ccagcgccgt
     8221 ggcgtagccg atacctttac ctgcgccggt tacccagaca tttttaccgc tgaaatccat
     8281 tatttcacct cgcgggagag tagcttccac caggcgtcga tggtcgggtt tttcgccagc
     8341 atgacaaagt cgatatcacc atgcactttg cgccagcgcg ccgccagcgc catcatgcgc
     8401 accgaatcca gaccgtagtc gatcaggttg tcgtcatcga acggttcatc ggactcgtcc
     8461 agcaacggca ggataacctc acgcagcgcc gctttgctgg cggggacagg tgctggcagt
     8521 aattcttcag tcatcaccac ccggccagac cgcccggcca catatttcag cgacatcaaa
     8581 tgctcgtcac ggctgaaatc ggccagcgca tccgccacca taaacggttt aatatcgcgc
     8641 ataaatgcgt cggttgcggt ggtcatacag ccaatgtggg catatacccc ggtaataatc
     8701 agctggttac gtccactctc tttgagcatt tgctccagcg gagaacgatg aaacgcgctg
     8761 tagcgccact tcaccagcac cgtgtcgtcg gcatctggcg tcaggcgatc caccaccttt
     8821 tgctgttccg gcgaacgggt caggcccggc ccccacatat cattcaacag cgcccgatct
     8881 tcatcgctct gctctttcgg ctgggcggtg taataaaccg gaatattgtg ctgtttgcag
     8941 tagtcgcgca gcgcagcaat attcgcgatc acctgctcca tcatcgggca gttctcgccc
     9001 cagaagctga caaaatagtc ctgcatatca tggattaaca acgcggcacg ttgcggttca
     9061 aaggcccagt caactttatt cttagggata tcgtgagact ccggcagtgc gtaagcctgt
     9121 aattttggaa tagccatcgt gttctctcct tcaggctgat gcgcgtgacg ccagccactg
     9181 acgtaattgt tttttatcga ctttcccgac cgccgtcagc ggaagtgaat ctacacactc
     9241 cacgcgatcc ggtaatttaa attcggcaat accctgttca cgcaggaaac gacgcacctg
     9301 caccgcgcgc agcggctctt ttaccaccag ataagcgcag cttttttcgc ccatcagctc
     9361 atcttccatg ctcaccagtg cggcgtagat caccgccggg tggcgcagca gcaggttttc
     9421 gatctcttcg gcagcgatct tctcaccgcc acggttgatc tgatctttct cgcgcccctg
     9481 cacggtgatg tacccctctg gatcaataga gatcagatcg ccggaacagt aaaaaccgtt
     9541 ggcatcaaag gcgctggcat tgtgctgcgg gcttttgtaa tagccacgga aggtgtacgg
     9601 accgcgggtc atcaggcgtc caacttcccc ttgcggcagt ggatttcctt cggcatcggc
     9661 aacccacact tcatcgtccg gacacattgg gtaaccctgg gtatggataa ttttctccgc
     9721 gctatcatca agacgggtgt agttcaccag cccttccgcc atgccaaaca cctgctgcaa
     9781 ctggcagcca atctcagcgg gaatacgcgc cgcaagggtg gcagaaagac gtgcgccgcc
     9841 gacctgtaac agtttcagcg aggcaagctg cgcccggctt tcaccttcgg tcagcgcctg
     9901 caaccacagg ctgactgccg gtggcaccag cgcggtgacg ttcacctgat gtttttcaat
     9961 caatgggaag caaagcgtgg cgctgggatc ggccgccaga acaaccgttc ctccggcaag
    10021 aaagacgccc agcgatcccg gcgaactcat ggcgtagtta tgagccgccg ggatcgcgca
    10081 caggtagcgt gtctgttgtg tgaactgaca aatctcgacg ctacgacgca cgctgtagta
    10141 gtagtcgtta tgagtgcgcg ggatcagttt cggtgtgccg gtggtgccgc cggaaagctg
    10201 gaaataggcc acttcatcag caggtgatgg cgtggcagta aaatcctcag ccggatggtt
    10261 aatcgcatcc tgcaagttat gctcaccgct gtcgttgtgc agttgcacca cgcgaatgga
    10321 ggaatgttct gtgacgaaag tattgaggaa atcatccccg ctaaacagcg catgttggcg
    10381 atcggcaatc agcaatgcgg gttcaatctg gctggcatag gcgttcagtt cactacgctg
    10441 atggctgaac agcgccagca ccggcgcaac gcccagtttc agcagcgcga aaaaggtaat
    10501 atacaattca gcgacgttac ccagttgtac cagcgcggtt tcaccaggtt taatgccctg
    10561 acggcgtaaa ctacacgcga ggttatccgc cgcctgattc agctcccggt aactcaactg
    10621 tcgctcgccg tcgataaccg cgatgctgtc actcgcagcg tggcgagtca gaatgtcggt
    10681 cagcggcaaa tcctgccagt agcctttttc ccgatagcga cgggcaaact cttccggcca
    10741 gcgggtgaat ggaatgctca tcctcgctcc ttaatgcaat ccaaaaacgt tcaacatggt
    10801 agaaagtttg acgcctgttt cgcgccactc acccaacggt gacgacgcag gcacaatccc
    10861 cgctccggca aacagacgca cctgattttc ccgcagcttc gcgcagcgga tggtcaccac
    10921 ccattcgccg ttaccttcgc tgtcacacca acccacaatg ccgccaaaca gttcgcggtc
    10981 gaatggctcc agttcagcaa taacctgggt cgcggcctga tgcgggaaac cgctcagcgc
    11041 gggggttgga tgcagcagac aggccagagt cagtgcgttt tcttgcgaat tcgctttacc
    11101 ttcaaaggga gttgcgagat gccacagcgt cggcgtggta atcaattgtg gagaggaggg
    11161 aacgtgtaac tcactactgc gttcgcgcag tacctctttc atcgcctgag tcaccagttc
    11221 atgttcatgg cgatcttttt ctgacgccag cagacgatta cccgcttcgc gatcgagcac
    11281 ttcatccggc tgacgacgcg cggaaccggc taacggaatg gagctaaaac gctcgccgtc
    11341 tttacgtagc agcagttccg ggctggcccc cagcaggacg ccaccatcag ccagcgggac
    11401 atggaagttg taactaaccg ggttttgcgc aatcaaccgt tccagcaata cgccactatc
    11461 aatggcggcg tcagtggtga tatcaatcaa tcgtgacaac accactttgt cgacctgcgg
    11521 cgtggcggta agtgcggcgg cgcgggcaac catctgttca aacgtggttt gttcaggaat
    11581 tgcctggcgt tccaccacgt tcagcgactg gctgcgggta aaacggcggg ctgaggtctg
    11641 tttttcctgg cgggagaacg actgccagga ttcgggaata tacagcgacg aaggctgacg
    11701 tggatcgaag ggaatcgccc cgaccatcac cggatttttg atgccctgcg ctttggcatc
    11761 ggcaaacagc gcggcgagtt tttgctggaa gggactgtcg ggcgaatccc cgttcacagc
    11821 cggttcatcg aagcgggcga aacatcctga cgtcgtaaaa ctgcggtacg gcgacataaa
    11881 gaaaaagcga ttgggcgcaa gtgttgccat ggtctgctgt acttcctcag ccagtgacgt
    11941 atccatatca tcctccacaa aatgataaag gctttaataa tgattatcat ttatattttc
    12001 ggtcgctaac ctaaaagcaa acgcgcacta tgtcaactct tgaggtaacg caatctgcaa
    12061 ctcggggttg catctggttg tggttacaat gaaaatgaga agcattattg atggattcgc
    12121 ataagcgcaa tgtgatgtcc tgcgccgttc tgccccctct cccttccagg gtgagggctg
    12181 gggtgagggt taatgttcgc accagtgctg gctgttcccc tcaccctctc cccaaagggg
    12241 cgaggggacg gattgtgcgc tttgtcgaat ttgtcattac gcccataacc ttattaataa
    12301 caggaagctg atttgtgaga ctcgccccgc tctaccgcaa cgcccttcta ttaacaggac
    12361 ttttgctttc aggaatagcc gcagttcagg ccgctgactg gccgcgtcag attactgaca
    12421 gccgtggcac acatacactg gaaagccagc cgcagcgtat tgtttccacc agcgtcaccc
    12481 tgaccggctc actgctggcg attgatgctc cggtgatcgc cagcggcgcg accacgccga
    12541 ataaccgcgt cgcggatgac cagggctttt tacgccagtg gagcaaggtg gcgaaagaac
    12601 gcaaactgca acggctctat atcggcgaac cgagcaccga agccgttgcc gcgcaaatgc
    12661 cggatctgat tttaattagc gcaaccggcg gggattcggc gctggcactg tatgatcagc
    12721 tttccaccat cgccccgaca ttaatcatca attacgacga caaaagctgg cagtcgctgt
    12781 taacgcagct tggcgaaatt accgggcatg agaaacaagc ggcagagcgg attgcacagt
    12841 ttgataagca actggcagcg gcgaaagagc aaatcaaatt accgccgcag ccggtcactg
    12901 ccattgtcta taccgccgct gcacacagtg ccaatctctg gacgccggaa tcagcacaag
    12961 ggcagatgct ggaacaactc ggctttacgc tggcgaagtt gcccgcaggg ttaaacgcca
    13021 gccaaagcca gggcaaacgc catgacatca ttcagcttgg tggggaaaat ctggctgctg
    13081 ggttaaatgg cgagtcacta ttcctgttcg ccggtgatca gaaagatgcc gatgcgattt
    13141 atgccaatcc actgctcgcg caccttcccg cagtacaaaa caagcaggtt tatgcgctgg
    13201 gaaccgagac gttccgtctg gattactaca gcgccacgca agtgctggaa cgacttaagg
    13261 cgctgtttta agcattaact gtcagacgct gtcacctgcg gcggcgtctg gcgaaaacgt
    13321 cgcaactcca ccagcaccag caataacaac acgccgataa tcaacaaacc aaaaccgctt
    13381 gcgcttgcgg aagcaaccgg tgtcatcatc gcgcccaaac cacccagcag cgccgcgcct
    13441 atggcatcgc ccgtcacgtt ctgcgccgtc cacaaaccgt taatccgccc taacatcgct
    13501 tccggggttt gcgtttgcag cattgtatat tgcagcaacg aactaaccgc gctcagccag
    13561 ccgaacagcg ccagacaaac cacgcctaaa atccacatcg gcatcaggcc aaacagacca
    13621 atggcgagga acgatcccag cgtggagagc agcatcaata accctggtcg cgcactatgt
    13681 gccagcttcc cgctggttaa cgcaccaata gccgcgccga gcgggatcgc cgcgtagaga
    13741 aaaccaatct gtgccgctga catctgccag ttgtcagcca gcgccggata cagtacccgc
    13801 accgcgctcg ccatcgtcaa taaaccaccc agcagcgcaa tcccgcccac cagcgggctg
    13861 gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcacg cggctgcggt
    13921 ggcggtggca acgccggaag gcttaacaac ggtagcaagg taataaacgt gcccgccgcc
    13981 gccagcccgt agttccaggc tacgccaccg gtcgccagca ataaaccgcc aatcatgggc
    14041 gaaatcaccg accccagacg cacggtcaac atggtgatcg ccccggcctg cattaagttt
    14101 tcacgcccta ccagtgctgg tgtcgccgcc aatagcgccg taacgccaag cgatgcgaaa
    14161 aaaccatccc ataaaccaag taaatagatt gccagcaatg acggctccgg cagcagtgca
    14221 ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa aatcactttt
    14281 ttgcgctcat agcgatccgc cagcacaccg ccaaccatca ggccaacaaa catcgcgccg
    14341 ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gcgtcatcat ctggatctgc
    14401 accggcaccg cgacgccgag caaacccaga gacacaattg agataaaacg agcgaggaat
    14461 actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca ggattgttta
    14521 ttcattacaa tgccttgcca tcaaatattt taaataccta ttcttaggct gcacatgcta
    14581 acatatccaa ataagataga taacgataat taatttcatt atcatggaag ttcgtatgtc
    14641 tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat tactgattat
    14701 cgcgacggca ttaagcctgc tcattggggc aaaatcactc cccgcttccg tagtgctgga
    14761 ggccctctcc ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg cgcgactgcc
    14821 gcgcaccctt gccggtttac ttgcaggcgg cgcgcttggc cttgccgggg cgttgatgca
    14881 aaccctcacc cgaaatccac ttgccgaccc cggcttgctt ggcgtgaatg ccggagccag
    14941 ctttgccatt gtgctgggtg cggcgctgtt tggttactca tccgcgcagg aacaactggc
    15001 gatggccttc gccggggcgc tggtggcctc attgattgtt gcctttaccg gcagtcaggg
    15061 cggcgggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg cggcggtgct
    15121 ggaagggctg accagcggca tcgccctgct taatcctgat gtctacgatc aactgcgttt
    15181 ctggcaagcc ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg tgctgatccc
    15241 ggtgctgatc gccggagcaa ccgcgctatt actgagccgc gcgctgaaca gtttaagcct
    15301 cggcagcgac accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt taattggtct
    15361 gctggcgatt accgtgcttt gtggtagtgc gacggcggta gttggcccga ttgcctttat
    15421 cggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc gctggtcgct
    15481 gcccgtcacg ctacttgcta cccctgccct gctgctgttt gccgatatca tcgggcgggt
    15541 gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg caccggtgct
    15601 gatcttcctc gtgcgacgta aaacgcgagg tggcgcatga tttacgtctc tcgccgatta
    15661 atcatcacct gtttgctgtt ggtttccgcc tgtgtggttg caggtatctg gggattacgc
    15721 agcggtgccg tcacgctgga aacctcgcag gtattcgccg cgctgatggg cgatgcgccg
    15781 cgcagtatga cgatggtggt caccgaatgg cgtttaccac gcgtgctgat ggcgctgttg
    15841 attggcgcgg cactgggcgt cagcggcgcg atttttcagt cgctgatgcg taacccgctc
    15901 ggcagccctg acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct ggtagcgatg
    15961 gtgctgtttg gtcaggacct gacggctatc gcgctggcag caatggtggg cggcattatc
    16021 acttcgctgt tggtctggct gctcgcctgg cgtaacggca tcgacacctt tcggttgatt
    16081 attatcggta tcggcgttcg tgccatgctg gtggccttta atacctggct gttgctgaaa
    16141 gcgtctttag aaacggcgct aacagcgggt ttgtggaatg caggatcgct caacggcctg
    16201 acgtgggcaa aaacctcgcc ttccgcaccc atcattatat tgatgctcat tgccgccgcc
    16261 ttactggtac gccggatgcg cttgctggaa atgggcgatg ataccgcgtg tgcgctgggc
    16321 gtcagcgtcg aacgttcgcg tctgttaatg atgctggttg cagtggtgct taccgctgcg
    16381 gcaaccgcgc ttgccgggcc gatttccttt attgctttag tcgcgccgca cattgcccga
    16441 cgcattagcg gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg ggcgctgtta
    16501 ctgctggcgg ccgatctctg cgcccaacaa ctgtttatgc cgtatcaact tccggttggc
    16561 gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca ggagtctcgc
    16621 aaaaaatgaa cgaatctgta gcccgtttgc gcggcgaaca gttaaccctc ggatatggca
    16681 aatataccgt tgcggaaaat ctgactgtag aaatacctga tggtcacttc acggcaatta
    16741 tcgggccaaa tggctgcggt aaatccactt tactgcgtac tttaagccgc ctgatgacgc
    16801 ctgctcacgg gcatgtctgg ctggatggcg agcacattca acattacgcc agtaaagagg
    16861 ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccaggcgat atcaccgtgc
    16921 aggagctggt ggcgcgtggg cgttacccgc atcaaccgct atttacccgc tggcgcaaag
    16981 aagatgaaga agcggtaacg aaagcgatgc aggccacggg aataactcat ctggcagatc
    17041 aaagcgtgga taccctttct ggcggacaac gccagcgcgc gtggatcgcg atggtgctgg
    17101 cccaggaaac ggcaattatg ctgctcgacg aacccacgac ctggctggat atcagtcatc
    17161 agattgatct actggaactg ttaagcgaac tgaaccgcga gaaaggctat accctggcgg
    17221 cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt gcattgcggg
    17281 aagggaaaat tgttgctcag ggagcgccga aggagattgt cactgctgaa ctgattgagc
    17341 gcatttatgg tctgcgatgc atgatcattg acgatccggt agccggaacg ccgcttgtgg
    17401 tgccgctcgg acgaacggca ccttcaaccg caaatagtta aactaagtgg tctgccatca
    17461 tggcatcctg ttttctggat gccatcgcat agcgcaataa cacgctacca caagccacca
    17521 tcccgccgat cagcgcggaa aggatcacaa taatcgcctt acccggaccg tcttttttca
    17581 ctggcaatga cgggcttaac tgatatttaa acggcgtaaa attcacatcg ttgatatttg
    17641 cttttgttaa ctgctcaaca agatactgac gattacgtaa ttcaccgttc agttccgcaa
    17701 cgtcagtgac cgctttttca atttccagtt tgcgttcaat accgtctgcg ccgagagaaa
    17761 tagagaaatc ggggtcatct ttaacggcct gaccattact gtatacgggc tttttaattc
    17821 ccgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca tcaagttgat
    17881 ttttcgtttt aatgcgatcc tgagccagtt tttctttttc aaactgggtt ttgatttcca
    17941 gtttattacg gacgttttct agtgactctt tcaccaccaa cgcagagata taatcgatat
    18001 acccgctcaa aacggtctgc gcctcttcac tggttggcgc ggtaaaactt agcgtccagg
    18061 aggtatacag tgacggttca tcttttttct tactggcatt gtcatcaacc gctttcattt
    18121 tttcgctcaa tgcaacaata gcgcgatgca aatccagttc atcgattttc gcctctttta
    18181 attggtccat cacataaggt gatgaacgca ggtactcttc cagcaagcta accgactgaa
    18241 acttcttgat aaacaggtta aatgcttctg tacgatcaat tttgatatcc agatccagca
    18301 ctcgaagctt agtgaatgtt ttctccagct cttgccactg aacaggttct ggaggcgtga
    18361 caaccgccgc gctggtccat ttttgcggca ggatgaaaga gatcagcaag cctgcgcagg
    18421 caaacgcaaa aatgaccgcc atgaccgttt ttttggcccg ccataaaact gcgattagat
    18481 taagtaaatc aatttcatta ttactgggcg acgccagagg atactcagga aaatgagcgt
    18541 cacttccctg tttaatattc agtgatgaca tggggtataa cctgaaaatg agtccaatag
    18601 gggaaatctc cacggcgctc actttaccag ttggtgataa ttatccgaag ctgaagtttg
    18661 taaattcctt ccactgaatg caacattcac tctatttaag agaaatattc atcaaaccta
    18721 taaatcaaac aaataaaaaa acccataatt attttctgta attatgggtt tatagatatt
    18781 attaatttac ctgtttagcg ttgcgcgaat aatcggccca atttttacaa acgcccctgg
    18841 agagataata tccacatgcg cacaatcctg acgatagata tccagctccg caatccacgg
    18901 cgaccaggca cgttcgggac tcataccttc ctgaagtgtg cgctcagcaa caaataacgt
    18961 tgcttttcca tcaaacggaa cgctatgggc ggtggttaac aagcgcacag catcagcgta
    19021 gttgccttca atggtggtaa acaactccgt tgaagtactt ccctgctgtg ccgccaggaa
    19081 ggcctcgcgt tcgcggttaa tctccgccag cacttccggg tccagaccat tagcctcttt
    19141 ttcctgccag ttttgcgtct ctggcggcca ggtatccagc aaaccaagaa atgccacctg
    19201 ttcgccacgg gcacgcagtc gcgccgcaat accctgcgcc agcgtaccgc caagggaata
    19261 ccccagcagg taataagggc cgtgcggttg ttgctcaagt aacgttgcca gatgcgcttc
    19321 gcagacttca tccaggtttg tcgctgtctg catgggtcca tgagggcgcg gcgactgaat
    19381 gccaataatc gaccattgtg gatcgagata acgcgagagc acgctaaact gccaggcaaa
    19441 accggacgcc ggatggaaac aaaacagcgt cgggccatta ccttcacgca acggcagaat
    19501 ggtttcgaat cccatgcgcc gggagctgtc ctcttcacca tcaataatcg ttgccagttt
    19561 ggcgacggtt gacgcgacca tcacctgccc cggcgtcacc tgacgagcaa actgtcgact
    19621 taactgcgct gctagtttca ttgccagtag cgaatgaccg cccagcgcaa agaaatcagc
    19681 atcggcatcc tgcacgtcac aacccagcaa cgacgcgaat gccgcggcga taatcgtttc
    19741 actgcccgct ttcggtgcac gtcccgacgc ttgtgtcttc agttcaggca acggtaaggc
    19801 tttgcgatcc agcttgccgt tggcgctaag cggcaactgc ggaagttgca gcagaacgac
    19861 cggcaccata tgcggcggca atgtttcgcg aagctgtgcc tgtaatgcgc tggtatccaa
    19921 cggcagtccc gattgcgaca ccagatagcc caccaactga cgcgcatcac caccggtggc
    19981 tgccgcctgg ttaatcacac aggcgtgggt aactgcctgt tcgacatccg gcagcgcctg
    20041 catcacgcga tcgatttcgc ccagttcgat acgctgcccg cgaattttta gctgatcgtc
    20101 actgcgcccg aggtactcca ccgcgccgtt atccagccag cgggcgacgt ctccggtacg
    20161 atacatccgt tcacctgggg caaaaggatc ggcaataaaa cggctggcgg tcagatcggg
    20221 tcgtccaaga tacccctgcg ccagttggat gccggtgaga tagagatcac ccgccacacc
    20281 cggcggcacc ggatgcatca tcgcatcaag aatacgcaga cccgtattcc ataccggata
    20341 accaatcggc acactgctgc cgcgcacctg tgccagttcc tcgccaaaag ccggatacca
    20401 gctgacatct accgccgctt ccgtcgggcc atacaggtta tgcaacggcg cgccggttaa
    20461 ctgttgccat tcgcggcata aatcagccgg taaggcctca ccactacaga aaacctgttt
    20521 caacgtcgcg caattctggc gagcggtttg cggcgtcagc gatgcaacaa atgccgccag
    20581 catcgacggc acaaagtgcg tggtcgttac gccatattcg gcaaagaatt gctgcatagc
    20641 gagcgggtca cggtgcgctt ccggttcagc catcaccagt tttgcccctg cgataaacgg
    20701 ccagaaaaac tcccacaccg agacatcaaa actgcacggc gttttttggg caacgacatc
    20761 ttcgcctgta agcgggtagt gattttgcat ccataacagg cggttaacga tagccgtctg
    20821 cccgaccatt acccctttcg gcctgccggt ggagccagag gtaaagatga tataagccgt
    20881 gtggtgcggt tgtgaaagtt gcagcggcgc actgccctgg ggggtaagcg gggcgttata
    20941 gcaaaggctt gttaaattgg gaacatcgct aaagcgcggc agttgatcgt cggtggtaat
    21001 taacagcgac ggacgcgcat cttccagcat cattttcagg cgatcgtccg gatagccggt
    21061 atccagcggt agccaggccg cgccagcttc aactattgca tggagtgcca gggtcaaaaa
    21121 gaccgagcgc ggtagtgcca ccgccacgct gtcccctggt ttaacgccgc gctcacgcag
    21181 cagattcgcc agcgccacca cctgctcgcg catttcccga tagctgaaca ggtaacgcgc
    21241 atctgccagc gccggagcat ccggtgtttt tgccgcttgt tctgccacca gcgcgctcag
    21301 cgtggtttct ggaatctcaa cctgagtggc gttgatctgc gccagctgcg catactcacc
    21361 tggcagcata atatcgacat cgccgcacaa cagcgccgga tccgcggcga actgggcaat
    21421 cagcattttc aggcgttcag catgctggat taacgttggc tcatcgtaac gctgtttatt
    21481 ggcgaggatc tcaatactca aatcaccgtg ttcatccggg aacagggcca gttcaaggtc
    21541 attaaccgga ccggttgcca gggtatgggt ttgcgtctga acaccaggaa tatccagttg
    21601 gtaatcaaat accttgatat tgagtaccgg accgaacagc ggttcctctc ctgccgctcg
    21661 cccgctgtca cggacaattt gttcggcatc gtaacgttga tgacgacgca tttttttcag
    21721 ttgtgctgcc agtcgggttg ccagttccgg cagcgtttct tgcgccgcaa tgtgaatacc
    21781 caacggtaaa acgttgagca cgggtccgct agccgtcagc gccgccgagc ccagtcgacg
    21841 cataaagata aatccggcgg catagtccat gcgattgcac aatcgcccca gccacaaggc
    21901 tgccagcgca agggctaaat cggtacgctg cacacctgaa agttgcgtag ccagctggcg
    21961 gaattccccg tcggtaaatt ccagtttcag gcgcagaata tctgccgaag cgctgcgccc
    22021 cgctaaaggt gccggagaaa gtgacgcggg cggcggcagt tgacgacgct gttctgccca
    22081 gaatgccgca tcacgctgcc aggcttcgct ttcgcggtat tgctggtact cttccactac
    22141 atcagcgaaa ggcgtaaacg gcgaagcagg cgttggttcg ccacgcagcc atgtgcagta
    22201 aatattggcg atctggcgag taatggccgg gaaactgaag ccatcgacca gcaaatggtg
    22261 ataacgctga taccagtacc agcggttatc cgcaacatgt atcagctgat gaaagaccag
    22321 tggtttaccg ctatcgacgc gcaaatcttg ttgcaaatcc gccagcatta atgcctgcgc
    22381 agtaccgtgc ggatcaatat tggttcgcag gtcgataatt tctggcagtt cgaatatcag
    22441 cgcatcatcg acccactgcc agacttcgcc gttatcttcc gtaaaacgca tccgcaacgt
    22501 atctgcttgc gctagtccga caaccaccgc gcgggccagt aatggcgcat caacatctcc
    22561 agttaactca acgtaatgcg ccacgctcca ggcggagggt aaatccgaca gtttttctgc
    22621 catccagatg ccgggctgtg cggcgaccaa aggtaaatgc tggctcattg tgcctcctgc
    22681 aactgggtga aattggccgg tgtcagagta cgccagtgga cttccagcca ctgctggcag
    22741 gacgcctgtg actgcggctg acacacaatg tcccagcctg ccggtaaggc gcattgttgc
    22801 ggccacagac tgaattgccc ctgcgcattg cgcaaaatgt aaaacgctcc ctgtggatca
    22861 tcgaagggat tactgaatgc catattcaac tcctgtcatg gaaagtggtt gccagaggtc
    22921 gattagcccc tgcatcaagc caccgcgcca acaaagcgca tcatgtccgc cgtcaacctg
    22981 acgccagaaa atggattctt ttatggggtg taattgcgca tacagcgcct gattggcccg
    23041 cataatcatc ggctcgcgaa tacccgcttc cagcacaatg cgcagacctt cggcgctaac
    23101 ttcaccagct ttaagctttt caagtaacac gccctcttgc tgcccgcccc gatgcggcca
    23161 ccagtacgat cctgactggc ttaatacaca gccaaagcgt tcaggccagt gcagtccggc
    23221 atacagcgcg gacagcccac caaaactctg cccggcgacc acggtgcgat cggcgcgatc
    23281 gctaaaaggg gcaatagctt tcaccagggg taataactct tgctgtactg ccagccagaa
    23341 atccgcatta cacggcagtt cgtgggcgcg gtgcgtggtg tcgatagcgt cgatcaacac
    23401 atacacggcg ggaggaagtt gctgacgatg ggtcagcgaa gtcagcacag gccagacggg
    23461 catactttgc gcccaaaatt cgccatcgag caaaactgcc agcggacgtt cttctgctgt
    23521 tgcatcgccg gtggtaaaaa tccatacacg acgtgaattt ttcaaccgtt cacttttcca
    23581 gataatttct ttggctgcta tttctggcgc ttgcggacaa tcccatcccg gttgcagagg
    23641 cgcttgcggc atttcgagtg cagaaacagc gtgccctcgc ccgcctttcc agctttgtgg
    23701 attcagcgga tcggctattg cctggggtaa tagttttcgc cagccttcac gtaattcgag
    23761 gcgatcgggg gatggtgcag aaaaaatgtc atcgcgttcg gtgggaataa agcagtagct
    23821 gccgcgccag ttggcattga gttgtgtcgt ccactgccag acatcagtac ctgcaattcg
    23881 ctgcatcgac tggggctggc tgttctgatg atgatcggtc acaccagtga tgtagatcca
    23941 tacgcgcttt atcgtcgagt attcttcaga accttgggga tcacgccacc agaaagtgac
    24001 ctcaaacatt tcgtcattca gacgctgcca ttccgggcca tgtttcgact gccaccagct
    24061 ctcacttcct acttttaacg ccgtcacacc ataaccccat gtttactgtg caatttttca
    24121 ttgattgcag aaatatattg ataatattat tgataactat ttgcatttgc aatagcgtaa
    24181 tggcgcgccg tgggaagcgc ggacatttat taaccaactg cactgcgtgt ctttcaggat
    24241 caaaggtttt cgcggtagcg ggatgcgtcg tgttgaggac gaccatgccc gacagttgca
    24301 attcgtggca aaaatgcagg aataaaacaa tgaacaagaa gattcattcc ctggccttgt
    24361 tggtcaatct ggggatttat ggggtagcgc aggcacaaga gccgaccgat actcctgttt
    24421 cacatgacga taccattgtc gttaccgccg ccgagcagaa cttacaggcg cctggcgttt
    24481 cgaccatcac cgcagatgaa atccgcaaaa accccgttgc ccgcgatgtg tcggagatca
    24541 tccgtaccat gccaggcgtt aacctgaccg gtaactccac cagtggtcag cgtgggaata
    24601 accgacagat tgatattcgc ggtatgggtc cggaaaacac gctgattttg attgacggca
    24661 agccggtaag cagccgtaac tcggtgcgtc agggctggcg tggcgagcgc gatacccgtg
    24721 gtgatacctc ctgggtgcca cctgaaatga ttgaacgtat tgaagttctg cgtggtccgg
    24781 cagctgcgcg ttatggcaac ggcgcggcgg gcggcgtggt taacatcatt accaaaaaag
    24841 gcagcggtga gtggcacggc tcctgggacg cttatttcaa tgcaccagaa cataaagagg
    24901 aaggagccac taaacgcacc aacttcagcc tgaccggtcc gctgggcgac gaattcagct
    24961 tccgcttgta tggcaacctc gacaaaaccc aggctgacgc gtgggatatc aaccagggcc
    25021 atcagtccgc gcgtgccgga acgtatgcca cgacgttacc agccgggcgc gaaggggtga
    25081 tcaataaaga tattaacggc gtggtgcgct gggacttcgc accattgcaa tcgctggaac
    25141 tggaagcggg ttacagccgc cagggtaacc tgtatgcggg cgatacccag aacaccaact
    25201 ctgacgctta cactcgctcg aaatatggcg atgaaaccaa ccgcctgtat cgccagaact
    25261 acgcgctgac ctggaacggt ggctgggata acggcgtgac caccagcaac tgggtgcagt
    25321 atgaacacac ccgtaactcg cgtattccgg aaggtctggc gggcggtacc gaagggaaat
    25381 ttaacgaaaa agcgacacag gatttcgtcg atatcgatct cgatgacgtg atgctgcaca
    25441 gcgaagttaa cctgccgatt gatatcctcg ttaaccagac gctgacgctg ggtacggagt
    25501 ggaatcagca acggatgaag gacttgagtt ccaataccca ggcgctgacc ggggcgaaca
    25561 ccggcggcgc tattgatggt atgagtgcca ccgaccgtag cccgtattca aaagcagaaa
    25621 ttttctcgct gtttgccgaa aacaacatgg agctgactga cagcaccatc gtaacgccgg
    25681 ggctgcgttt cgatcatcac agtattgtcg gcaataactg gagcccggcg ctgaacatat
    25741 cgcaaggttt aggcgatgac ttcacgctca aaatgggcat tgcccgtgct tataaagcac
    25801 cgagcctgta ccagactaac ccgaactaca ttctctacag taaaggtcag ggctgctatg
    25861 ccagcgcggg cggctgctat ctgcaaggta acgatgacct gaaagcagaa accagcatca
    25921 acaaagagat tggtctggag ttcaaacgcg acggctggct ggcgggcgtc acctggttcc
    25981 gtaacgatta tcgcaataag attgaagcgg gctatgtggc tgtagggcaa aacgcagtcg
    26041 gcaccgatct ctatcagtgg gataacgtgc cgaaagcagt ggttgaaggt ctggaaggat
    26101 cgttaaacgt accagttagc gaaacggtga tgtggaccaa taacatcact tatatgctga
    26161 agagtgaaaa caaaaccacg ggcgaccgtt tgtcgatcat cccggagtac acgttgaact
    26221 caacgctgag ctggcaggcg cgggaagatt tgtcgatgca aacgaccttc acctggtacg
    26281 gtaagcagca gccgaaaaaa tacaactaca aaggtcagcc agcggttggc ccggaaacca
    26341 aagaaatcag tccttacagc attgtgggtc tgagcgcgac ctgggatgtg acgaagaatg
    26401 tcagtctgac cggcggcgtg gacaacctgt tcgacaaacg tttgtggcgt gcgggtaatg
    26461 cccagaccac gggcgatctg gcaggggcca actatatcgc gggtgccggg gcgtatacct
    26521 ataacgagcc gggacgtacg tggtacatga gcgtaaacac tcacttctga tgctaacgtc
    26581 agattgttga caaagtgcgc gtcgttcatg ccggatgcgg cgtgaacgcc ttatccggcc
    26641 tacaaaaatc ttgccaattc aatatattgc aggaccatgt aggcctgata agcgtagcgc
    26701 atcaggcagt ttagcgtttg tcatcagtct ctaatatggt cgacatgaaa actacgcata
    26761 cctccctccc ctttgccgga catacgctgc attttgttga gttcgatccg gcgaattttt
    26821 gtgagcagga tttactctgg ctgccgcatt acgcgcaact gcaacacgct ggacggaagc
    26881 gcaaagccga acatttagcc gggcggatcg ctgcaattta tgcgctgcgg gaatatggct
    26941 ataaatgtgt gcccgcaatc ggcgagctac gccaaccggt gtggcctgcg gaggtatacg
    27001 gcagtattag ccactgcggg gctacggcat tggccgtggt ttcccgtcaa ccgattggcg
    27061 ttgatatcga agaaattttt tctgcgcaaa cagcgaccga attgacagac aacattatta
    27121 caccagcaga acacgagcga ctcgcagact gcggtttagc cttttctctg gcgctgacac
    27181 tggcattttc cgccaaagag agcgcattta aggcaagtga gatccaaact gatgcaggtt
    27241 ttctggacta tcagataatt agctggaata aacagcaggt catcattcat cgtgagaatg
    27301 agatgtttgc tgtgcactgg cagataaaag aaaaaatagt cataactctg tgccaacacg
    27361 attaattgac aacatctggt acgattcgcc cgcagccatc actgaccgcg ggcgaaagtg
    27421 taaagcaggt gccttaccac tctgacctga caaccggata tgcggggatt gctccccgca
    27481 caacggctac ttcttcggtt cgtaagcgag aacagcctta aactcaatat tacgttcctt
    27541 tacggtgaac tcacacagcg agtctccgac cagaagcgta aatcccagta ccgtcaaaca
    27601 cagcactatt actgccacaa gggcatattt cgtcagcatg tttatattgc ctccatgcga
    27661 agaagggact accatccgag ttgttgaggc tcggagtggc agccccagga ttgatagaaa
    27721 tatctcctgg ggcttcatct ttccagacct cagagttaac ctgaaaccgg aaagcttcag
    27781 gcacccgccg ttatcttacc cttcctcaac aaaatatctg tttatttata cagtgaatcc
    27841 tgtttcggac ttctgtcggc aaaactcatc attgctctta aaacgaaatc gcccgcagcc
    27901 atcactgacc gcgggcgaaa gtgtaaagca ggtgccttac catcctgacc tgacaaccgg
    27961 atatgcgggg attgctcccc gcacaacggc tacttcttcg gttcgtaagc gagaacagcc
    28021 ttaaactcaa tattacgttc ctttacggtg aactcacaca gcgaatcccc gaccagaagc
    28081 gtaaatccca gcaccgttaa acacagaact atgactgccg caagggcata tttcgtcagc
    28141 atgtttatat tgcctccatg cgaagaaggg actaccatcc aaattgttca ggtttgggta
    28201 ggtagcccca gggttgatag aaatatcgtc ctgggacttc atctttccag acctcagagt
    28261 aaacctgaaa ccggaaagct tcaggcaccc gcagttatct tacccttgct tagcaaaata
    28321 gctgtttatt tatacagtaa gtcctgtttc ggacttctca ctccccattt gatcaactga
    28381 agctgaaccc gtttttttct gcatgcctca atcgtcaaaa ttcttcgcca ccagccagcc
    28441 gtgccataac ttgtcacatt gcaaaagtag ccaaattgct tcagtcacca tcaccaaaaa
    28501 aagaaacatt ccagaacttc cgcatctcgc ccagccgctc cgacttctat actgaataga
    28561 aaacgccaac ttaagagaaa cctatgccat tacccgattt tcatgtttct gaacctttta
    28621 ccctcggtat tgaactggaa atgcaggtgg ttaatccgcc gggctatgac ttaagccagg
    28681 actcttcaat gctgattgac gcggttaaaa atgagatcac ggccggagag gtaaagcacg
    28741 atatcaccga aagtatgctg gagctggcga cggatgtttg ccgtgatatc aaccaggctg
    28801 ccgggcagtt ttcagcgatg cagaaagtcg tattgcaggc agccgcagac catcatctgg
    28861 aaatttgcgg cggtggcacg cacccgtttc agaaatggca gcgtcaggag gtatgcgaca
    28921 acgaacgcta tcaacgaacg ctggaaaact ttggctatct catccagcag gcgaccgttt
    28981 ttggtcagca tgtccatgtt ggctgcgcca gtggcgatga cgccatttat ttgctgcacg
    29041 gcctgtcacg gtttgtgccg cacttcatcg ccctttccgc cgcgtcgccg tatatgcagg
    29101 gaacggatac gcgttttgcc tcctcacgac cgaatatttt ttccgccttt cccgataatg
    29161 gcccgatgcc gtgggtcagt aactggcaac aatttgaagc cctgtttcgc tgcctgagtt
    29221 acaccacgat gatcgacagc attaaagatc tgcactggga tattcgcccc agtcctcatt
    29281 ttggcacggt ggaggttcgg gtgatggata ccccgttaac ccttagccac gcggtaaata
    29341 tggcgggatt aattcaggcc accgcccact ggttactgac ggaacgcccg tttaaacatc
    29401 aggagaaaga ttacctgctg tataaattca accgtttcca ggcctgccgc tatgggctgg
    29461 aaggcgtcat taccgatccg cacactggcg atcgtcgacc gctaacggaa gataccttgc
    29521 gattgctgga aaaaatcgcc ccttccgcac ataaaatggg tgcatcgagc gcgattgagg
    29581 ccctgcatcg ccaggtcgtc agcggtctga atgaagcgca gctgatgcgc gatttcgtcg
    29641 ccgatggtgg ctcgctgatt gggctggtga aaaagcattg tgagatctgg gccggtgact
    29701 aagtagggaa ttgccttaca gaagaatatc cctgacaatg gtgttttaac ttacatttaa
    29761 caatgttatg aaacaccctt tagaaacctt gactaccgca gcaggcattt tgctgatggc
    29821 tttcctctct tgcctgctgc tgcccgcccc cgcactgggg ctgacgctgg cacaaaaact
    29881 ggtgaccatg ttccatctga tggatcttag ccagctttac actttattgt tttgtctgtg
    29941 gtttttagtg ctgggcgcta ttgagtattt tgttctgcgc tttatctggc aacgctggtt
    30001 ctcgctggcg gattaaacgt cgattatcgc cgtacttcgc ggcatacttt gcttattctc
    30061 cttcgcctaa aggaacgttt atggataagc aatcactgca cgaaacggcg aaacgcctag
    30121 cccttgagtt accctttgtc gagctttgct ggccttttgg cccggagttc gatgttttta
    30181 aaattggcgg caaaattttt atgctgtcgt cggagctgcg cggcgtcccc tttatcaatc
    30241 tgaagtccga tccacaaaaa tccctgttaa atcagcagat atacccgagc attaagccag
    30301 ggtatcacat gaataaaaag cactggattt cggtgtatcc cggcgaggaa atctccgaag
    30361 cgttacttcg cgatctgatc aacgattcgt ggaatctggt ggttgatggt ctggctaaac
    30421 gcgatcaaaa aagagtgcgt ccaggctaaa gcggaaatct atagcgcatt tttctcgctt
    30481 accatttctc gttgaacctt gtaatctgct ggcacgcaaa attactttca catggagtct
    30541 ttatggatat catttctgtc gccttaaagc gtcattccac taaggcattt gatgccagta
    30601 aaaaacttac cccggaacag gccgagcaga tcaaaactct cctgcaatac agcccatcca
    30661 gcaccaactc ccagccgtgg cattttattg ttgccagcac ggaagaaggt aaagcgcgtg
    30721 ttgccaaatc cgctgccggt aattatgtgt tcaacgaacg taaaatactt gatgcctcgc
    30781 acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc ctggctgaag ctggttgttg
    30841 accaggaaga tgctgatggc cgctttgcca cgccggaagc gaaagccgcg aacgataaag
    30901 gtcgcaagtt cttcgctgat atgcaccgta aagatctgca tgatgatgca gagtggatgg
    30961 caaaacaggt ttatctcaac gtcggtaact tcctgctcgg cgtggcggcg ctgggtctgg
    31021 acgcggtacc catcgaaggt tttgacgccg ccatcctcga tgcagaattt ggtctgaaag
    31081 agaaaggcta caccagtctg gtggttgttc cggtaggtca tcacagcgtt gaagatttta
    31141 acgctacgct gccgaaatct cgtctgccgc aaaacatcac cttaaccgaa gtgtaattct
    31201 ctcttgccgg gcatctgccc ggctatttcc cctcagattc tcctgatttg cataaccctg
    31261 tttcagccgt catcataggc tgctgttgta taaaggagac gttatgcagg atttaatatc
    31321 ccaggttgaa gatttagcgg gtattgagat cgatcacacc acctcgatgg tgatgatttt
    31381 cggtattatt tttctgaccg ccgtcgtggt gcatattatt ttgcattggg tggtactgcg
    31441 gaccttcgaa aaacgtgcca tcgccagttc acggctttgg ttgcaaatca ttacccagaa
    31501 taaactcttc caccgtttag cttttaccct gcaggggatt atcgtcaata ttcaggcggt
    31561 attctggctg caaaaaggca ccgaagcggc agatattctg actacctgcg cgcagttgtg
    31621 gatcatgatg tatgcgctgc tttcagtctt ctcgttgctg gatgttattt tgaatctggc
    31681 gcagaaattc ccggcggcat ctcagttacc gctgaaaggg atatttcagg ggattaaact
    31741 gatcggcgca atcctggtcg gcattttgat gatctcgctg ctgattggtc agtcgccagc
    31801 gattctgatc agcggtcttg gtgcaatggc tgccgtgctg atgttggttt ttaaagatcc
    31861 gattcttggt ctggtggcag gtattcagct ttccgcgaac gatatgctga aactgggcga
    31921 ctggctggag atgccgaaat acggcgcgga tggcgcggtg atcgatattg ggttaaccac
    31981 cgtcaaagtg cgtaactggg acaataccat taccactatt cccacctggt ctctggtttc
    32041 tgactccttt aaaaactgga gcgggatgtc agcatctggc gggcgacgta ttaagcgcag
    32101 tatcagtatt gatgtcacca gtattcgttt tcttgatgaa gacgaaatgc aacgtctgaa
    32161 taaagcgcac ttgttaaaac cttatttaac cagccgccat caggaaatta atgagtggaa
    32221 tcgccagcaa ggttctacgg agtcgatatt aaacctgcgc cgaatgacca atattggaac
    32281 ctttcgtgcc tatctgaacg aatatctgcg taaccatccg cggattcgta aagatatgac
    32341 cttaatggta cgccaactgg ctccaggtga taacggttta ccgctcgaga tctacgcgtt
    32401 tactaacacc gtggtgtggc tggaatatga aagcattcag gctgatatat tcgatcacat
    32461 atttgcgatt gtcgaagagt ttggtctgcg acttcatcag tcgccaaccg gcaatgatat
    32521 tcgctctctg gcgggtgcat ttaagcagta attaaaaaaa ccgctctcat cgaatggatg
    32581 agagcggaca tcgttttgtc ggatgtagcg tgaatgcctt atttccgacg cagcgtttta
    32641 aatgccacaa acaggaatac aatccacacc ggcagcagga tcgctgacaa gcgcatatca
    32701 tccatcgtgc acatcagcag caaaatcatg ccgaggaagg caatgcaaag atagttgccg
    32761 aacggataga gcagcgcctt aaactgtgtt tcacgcccct gacgtcgcat cgctgcacga
    32821 aaacgcagat gcgccagaca gatcataatc cagttcaaca gcagcgttgc taccaccagc
    32881 gccatcagca gaccaaacgc tttttgcggc agcagatagt tgattaatac caccagcgaa
    32941 gtgatcgctc cggaaagcat cagcgagtta atcggcacac cgcgacggct gacgcgagtc
    33001 aaaaactttg gcgcattacc ctgcacagaa aggccaaaca gcatgcggct gttagagtaa
    33061 accccgctgt tatacactga cagcgatgct accagaatga cgaagttcag cgcagaagct
    33121 accacgttgc tgtcgagatt atggaaaatc atcacaaacg ggctactgtt ggatttcact
    33181 tccacccacg gatagagcgc cagtaaaacc accagtgaac cgatgtaaaa cagcaggatg
    33241 cgatacacca cctgatttac cgcttttgga atgctttttt ccggatcgcg cgcttcagcg
    33301 gcagtaatcc caatcagctc cagaccgccg aaggagaaca taattaccgc cagcgacaaa
    33361 atcagcccat tccagccggt ggcaaagaaa ccaccgtagc gccagaggtt gtcgatactg
    33421 gctttctcgc cgccgtgacc agaaaacagc agccacaggc caaagccgat cataccgatg
    33481 atcgccagca ctttaatcag cgcaaaccag aactcggttt caccatataa gcgcacgttc
    33541 accaggttaa cggcgttgat gataataaag aaggcggcag cccaaatcca cgttggaaca
    33601 tccgggaacc agtactgcat atagatgccc gcagcggtca gctctgccat tcccaccagc
    33661 acgaacatta cccagtagtt ccagccagag aggaagcccg caaacggtcc ccagtattta
    33721 taggcaaagt gggcaaatga accggatacc ggctcttcga ccaccatctc gccaagctgg
    33781 cgcataatca ggaaagcgat gatcccggcg acgccgtagc ccagcaatac agccggaccc
    33841 gccatctgaa tcgccgggcc aatgccaaga aacagaccag taccaattgc gccacccagc
    33901 gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa gcgtcggctc ttgattcgac
    33961 gcagtatctt ccgatacggt tgacgcgttt ttcacgcctt tcccctgtgt gtcttttttg
    34021 ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa gacacaatcc acacggttaa
    34081 acggggtatc ctgcttttat ttccgtaccc gatgtcggtg cagccacatc agcttatacg
    34141 ccgccgggat aataaacagc gacagcaaag gtgcggtgat catgccgcca atcatcggtg
    34201 cggcaatccg gctcatcacc tctgaaccag cccccgttcc ccacagaatc ggcagcagac
    34261 cggcgataat caccgccacc gtcatcgctt tcgggcgcac gcgcaggacc gcgccgtgat
    34321 ataacgcctc atccagcttc tgctcgctga atgtttgtgg attattcaac gacggctcgg
    34381 cctctatggc gtgacgtaaa tacatcagca tcaccacgcc aaattcggcg gcgaccccgg
    34441 cgagggcgat aaagccagtg cccgtcgcca cggaaagatg aaagcccatc caccacagca
    34501 accagatgcc gcccaccagc gcaaacggta cgctgctgat aatcagcaac gcttcgccca
    34561 cccgacggaa cgccagatac aacagcacga agataatcat caacgtcatc ggcaccatga
    34621 gtttaagctt atggttggcg cgctccagta gctcgaactg cccggagaat gccacgctgg
    34681 tgcccggttt taactgcact ttttcagcta tcgctttttg caaatcgtga accaccgaca
    34741 ccatgtcacg atcgcgggca tcgatataaa tccagctcgt cgggcgcgca ttctcggttt
    34801 tcagcattga cggtccggta gagactttaa tgtcggccac gtctgccagg gtgatttgct
    34861 gcttcatcgg cgtcaggatc ggcaactggc gcagtgcctg cggactatcg cgccagcttt
    34921 gcggataacg cagattaatt ggataacggg caatcccttc caccgtttcg ccaaccatcg
    34981 ccccgcccac cgcagaagtc acaaacaact gcacatccgc caccgtcata ccgtaacgtg
    35041 cggctttttc acggttaatc tcaacgttga tatagcgccc accttccaga cgctcggcta
    35101 gagcagaagc cacgcctggc accgttcgcg ctacttcttc aatttgctca gccatcgcgt
    35161 cgatatccgc cagcacagtg ccggaaactt taatgccgat ggggctttta atgccggttg
    35221 agagcatatc gatacggtta cgaattggcg gcacccacag attcgccagc cccggcagac
    35281 gcacggtgtt atccagttcc tcaatgattt tgtccattgt catgcctggc cgccactgct
    35341 cctgcggctt aagctggatg gtcgtttcta ccatctccag cggagcagaa tcggtggcgg
    35401 tttccgcttt cccggttttg ccaaataccc gcgccacttc aggtacgctc ataattagct
    35461 tgtcggtttt ttgcagcata ctcgccgcct ctgctgcgga aatccccggc agcgtcgatg
    35521 gcatatacaa caggtcgcct tcattgatct gcggtaaaaa ttccccgcca actttattga
    35581 gcggccagag aaccgtcagc accgaaagcg ctgccaccaa cagcgtggtt ttcggccagt
    35641 gcagtacttt cagcaacagc ggatgataaa cacgaatcaa aaagcgattg agcgggttac
    35701 tgctttccgg cggaattttg ccacggatcc agtaacccat caggataggg atcactacga
    35761 tcgccagcag cgccgcaccc gccatcgcat acgttttggt gaacgccagc gggccaaaca
    35821 gacgcccttc ctgcccttcc agggtgaaga tcgggataaa cgacaacgtg ataatcagca
    35881 gactgataaa tagcgccgga cccacttcaa cagatgcatc ggtgatcacc tgccagcgcg
    35941 ttttattatc cagcgtggcg tcaggatgct ggtgctgcca ctcttccagc cgtttatgtg
    36001 cattttcgat catgacgata gcggcatcga ccatcgcccc gacggcaatc gcaatgccgc
    36061 ccaacgacat aatattggca ttcagtccct ggaagtgcat gacaataaaa gcaatacaca
    36121 accccagcgg caacgaaata atcgccacca gcgccgagcg cacatgccag agaaacagcg
    36181 cacagactac cgccaccaca ataaactctt ccagcaactt gccgctgagg ttgtcgatgg
    36241 cgcggtcaat gagctggctg cgatcgtatg ttgtaactat ctccacgcct tccggcagac
    36301 tacttttcag cgtttccagt ttgtccttca cggcggcgat cacttctcgg gcgtttttgc
    36361 cggatcgcag gatcaccacc ccgcccgcca cttcgccttc gccgtttagt tcggcaatgc
    36421 cccggcgcat ctccgggcca atctggacct tcgcaacatc gcgcagataa acgggcacgc
    36481 cattttcact ggcttttaaa acgatgtgat taaagtcgtc gagcgtttgc agatagccgc
    36541 tggcgcgcac catatattcc gcttccgcca gttcgatcga cgaaccgccc gcttcctggt
    36601 ttgaagcatc cagcgcgctt tttacttcgg cgagactgat gccatactgc gccaggcgct
    36661 ggggatcgat aaccacctga tactctttca ccacaccgcc caccgacgcc acttccgcaa
    36721 cgtcagggat ggttttcagc tcatatttga gaaaccagtc ctgcaatgag cgtaaatcgg
    36781 ccagatcgtg cttaccgctg cgatccacca gtgcatattc atagatccag ccaacacccg
    36841 tggcatctgg tcccagctcg gcgctgactc ccgcaggcag cttaccctgt acctggttga
    36901 ggtactccag cacccgcgaa cgcgcccagt acggatcggt gccatcttcg aaaatgacat
    36961 acacataaga gtcgccaaac tgtgagaaac cgcgcacagt cttcgcgcca ggcaccgaca
    37021 acatggtggt ggttagcgga taagtcacct gattttcaac gatttgtggt gcctgaccgg
    37081 gatagctggt tttaataata acctgcacat cggagagatc cggcagcgca tccactggcg
    37141 tattaatgat ggtccaggtg ccccagatgc tcagaaacaa cgcgcccatc agcaccaaaa
    37201 aacggttcgc caccgagcga cgaataatcc attcaatcat tggttattcc ctcaatgcgc
    37261 atgggtagca ctttcagagc gcatccgctc cagtgcgcca gaaatattgg cttcggaatc
    37321 aatcaggaac aggccgctgg aaaccacctt ttcaccttcc gccagaccag agcgtaatgc
    37381 ggtgacgcct tgcgatgcct ggaaaacagc aacgcgtttc ggtacaaagc gcccgtcggc
    37441 atcaacggta atcacccgct gttcgctgcc ggtatcaatc agcgcttgtg acggaatgag
    37501 cagcatcggt tcgctggcgg tgttgagttg caaccaggcg ttcatgcccg gttttagcgc
    37561 ctcgtcggcg ttgtcgactt ccagacgcag ctgcagcgtg cgggtcgcgg catccacgcc
    37621 aggtagcagc gtccatttgc ggatggtgag tgttttatcc ggtcgcgccg gaacggtgag
    37681 ggtaaactgc gaggcatctt tcaccagcca ggcgatggac tccgggatcg cagcagtgac
    37741 ccacaccggg tccataccct gaattttcgc gaccacgtta tctttggcga tattcattcc
    37801 cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggc gctttgagcg taaagcgagt
    37861 ctggattttt tgcgtggcga tcaggcggcg aatatccgcc tccggcattc ccgccagtcg
    37921 cagccgctca agaatgcctt cagtctgggt cgccgtaccg ccggtttcgc gcagcagtaa
    37981 atactcactc tgcgcttcca cccagtcagg aatggtcagg tcgagaagcg gtgtgccctt
    38041 ttgtacttta tcgcccacgg taagcggata caccttgtcg ataaacccgg cagcgcgagc
    38101 ctgcacaatg gcatactgat actcgttgta actgacattc gccgggaaac tctgggcaaa
    38161 agtcagcggc ccgcgcgtga cggtagccgt tttcaccccc agattctgcg tctgagtcgg
    38221 gtcaatgcgc acaccagacg cagaactctc ttcatcggca tatttcggca ccagatccat
    38281 atccataaac ggcgatttac ctggtttatc gaaccgcgta ttggggtaca tcgggtcgta
    38341 ccagaataag attttacgtt ctgcggtcga cgttttttct gcgggtggtt cagcctttgc
    38401 aaaccaggta aaacctgccg cagaaataat accgcccgcg atcatgctgc cgataataag
    38461 cgcgattttt ttcatctcat taaacctggg ttactggctg actttaatat cctgtaataa
    38521 agaaaggttg ccctgctgga caaaattaaa cgccactttg tcgccagttt taatttcact
    38581 cattttcgtc tgcggagtga tggtaaagcg catggtcatc tccggccagt tcacggcagc
    38641 aatcggatcg tgatggatgg tgattttttt gctttccaga tcaataccct ttaccacgcc
    38701 agtggcgcta ataacctgcg gttgtacttc gctcatggtt tcatgatgat gttcgttagc
    38761 ctgggcatta aagccaataa cggtaaacag actgaacatt gcgacttgca gtgctttttt
    38821 catttattct ctcctggagt taaaagtcat actctaaata attcgagttg caggaaggcg
    38881 acaagcgagt gaatccccag gagcttacat aagtaagtga ctggggtgag cgaacgcaga
    38941 cgcagcacat gcaacttgaa gtatgacgag tatattgctg tcaaccgcca cctaaggcgg
    39001 tatacaaaga aatttcgtta acctgacggg cataattcag gtcgagtaaa gtttgtcggg
    39061 ttgcaaataa agaacgctcg gcatccagca cttccagata acttactgcg ccgtgctgat
    39121 ataatgcccg cgcccgttgc aaagtaattt gcagcgacgc cagataacgc tgctgggcgc
    39181 tgatttgatc gttcaggctt tgacgtaatg ccagcgcatc tgccacttct ttaaaggcgt
    39241 tctggatttt ctgttcataa ttcactaccg actgctgctg gcgaatttcg gcgatatcca
    39301 gattggcctg gttgcgtccg gcattaaaaa tggggatctc aattttagga ataaaattcc
    39361 acatcccgct gctggcgtta aataatgacg acagatcgct gctggcggtt gatattccgc
    39421 tggtcagcct tatagatggg aaaaatgccg cgcgtgcggc cccaatattg gcattagccg
    39481 ccattaacgc gtgttcagct tccataatat ccgggcgctg caataagatt tgcgacgaca
    39541 atcccgccgg taatttaacg ctttgcaggc tgtcgctgtt tactgtctgc gcttgcggca
    39601 gcttgccgta gcttcccaat aacagttgca atgcattatt cgcctgcgcc agttccccct
    39661 gacgtttagc gatgtcgctg cgggtacttt caatcacccc gcgagcctgt tccagcgcca
    39721 gaacattact gctacctgtc aacagttgtt tttcgacaaa cgcatatgac tgctgataat
    39781 tacgcagcgt ttcttcggct atttgcaatt gcgcatacgc tagctgctga ttgaaataac
    39841 tttgcgcgac attagaaacc agcaaaatat gcaccgcgcg ctgggcttcc tcagtggcta
    39901 aataattttg gcgctcggct tcgctcatgt tctttaagcg accgaaaaaa tcgagatcaa
    39961 agctggcgtt aaggccggtc gaaaactccc gtgtcgtggc ggtattccct ttaagattac
    40021 cgctccagct gccgctgccc tcgccattga gctgtgggta gcggtcggca tcggtcagac
    40081 gatattgcgc ccgcgtttcc tgcactttca gcgtcgccat gcgcaaatcc cggttattca
    40141 ccagcgcctc gctaatcagc gtctttacct gattatcaac aaaaaaggtg cgccagccca
    40201 cgttctggta gttatctgct gcgttaacca agccgttctg gctgagtgag aactgctgcg
    40261 gcacgggcat tgccggacgc tgataatccg gtgccagtga acaaccggtt agcgcaaggg
    40321 ccacacaaaa tggcagaagt ttacaaggag acataggctc ataatttctg gtgattttat
    40381 accgccaact ttactcgcca ggctctgatt ttccggtgac aggaaaatga caaaattgtc
    40441 attttgccaa taagcgattg ccatctgatc ccgctactct agaattgccc gggcaacatg
    40501 cggaggaaaa atgaaactgt tgattgtcga agatgaaaag aaaaccggag aatacttgac
    40561 caaagggtta accgaagccg gttttgtggt cgatttggcc gacaacgggc tgaatggcta
    40621 ccatctggcg atgaccggtg attatgatct gataatcctc gatattatgc tgccggacgt
    40681 gaacggctgg gatatcgtgc gcatgttgcg ttccgccaat aaagggatgc cgattctgtt
    40741 gcttaccgcg cttggcacca ttgaacatcg cgtcaagggg ctggagttgg gggcagatga
    40801 ctacctggtg aaaccattcg cttttgctga actgctggcg cgggtgcgca ctctcctgcg
    40861 gcgcggggcg gcggtgatta tcgaaagtca gtttcaggtt gccgatctga tggtcgatct
    40921 cgtcagccgc aaagtcaccc gcagcggcac gcgcatcact ctgaccagta aagagtttac
    40981 tctgctggag tttttcctcc gccatcaggg cgaagtgctg ccccgctcgc ttatcgcctc
    41041 gcaggtgtgg gacatgaatt tcgacagcga cactaacgcc attgatgtag cggtgaagcg
    41101 gctgcgcggc aaaatcgaca acgactttga gccaaagcta attcagaccg tgcgcggcgt
    41161 gggttacatg cttgaggtgc cggatggtca gtaagccatt tcagcgcccg ttttcgctgg
    41221 caacccgcct gacctttttt atcagcctgg ccaccatcgc agcgtttttc gcctttgcat
    41281 ggatcatgat ccactcagta aaagtgcatt ttgccgagca ggatattaat gatttaaaag
    41341 aaattagcgc cacgcttgaa cgggtactta atcatcctga cgagacgcaa gcccgacgct
    41401 taatgacgct ggaagatatc gtcagtggtt attccaacgt gttgatctcc ctggcagata
    41461 gccacggcaa aacggtgtat cactcccccg gtgcgcctga tatccgcgag tttgcacgtg
    41521 atgccatacc cgataaagac gcgcggggtg gcgaggtgta tctcctttcc ggcccgacga
    41581 ttatgatgcc aggccacggt cacgggcata tggaacacag caactggcgg atgattaact
    41641 tgtcggttgg cccgttggtg gacggcaaac cgatttatac gctctacatc gcgctttcga
    41701 tcgattttca tcttcattac ataaatgatt tgatgaataa acttattatg accgcatcgg
    41761 taatcagcat cctgatcgtc tttatcgtac tgttggcggt acataaaggt cacgcgccga
    41821 tccgcagcgt cagccgtcaa atccagaata tcacctcgaa agatctcgac gttcgccttg
    41881 acccgcagac cgtgcccatt gagctggaac agctggtact gtcgttcaac catatgatcg
    41941 agcgtattga ggatgtcttt acccgccagt ccaatttctc agcggatatc gctcacgaaa
    42001 ttcgcacgcc gattaccaat ctcatcacgc aaacggaaat cgccctcagc cagtctcgca
    42061 gccagaagga gctggaagat gtgctctact ctaatctcga agagctgacg cgaatggcga
    42121 aaatggtcag cgatatgctg tttctcgctc aggccgataa caaccagcta atccccgaaa
    42181 agaaaatgct caacctggcg gatgaagtcg gcaaagtgtt cgattttttc gaggcgttag
    42241 cggaagatcg cggcgtggag ttgcgatttg ttggcgacga atgtcaggtt gcgggcgatc
    42301 cgctgatgct gcgtcgggcg ttaagcaacc tgctttctaa tgccctgcgt tatacgccgc
    42361 ccggagaggc aattgtagtg cgctgccaga cggtcgatca ccaggtgcaa gttaccgtcg
    42421 aaaaccccgg tacgcccatt gcgcccgagc acttaccgcg attgtttgac cgtttctacc
    42481 gcgttgaccc ttcccgccag cgaaaaggcg aaggtagcgg cattgggctg gcgatagtga
    42541 aatcgattgt tgtcgcgcat aaaggcacgg ttgcagtaac gtcagatgcg cgggggacaa
    42601 ggtttgtgat tatgctgccg gagagagagt gattattaag ttcaaattca gggcgtggtg
    42661 catcacctcc tggtattgtt gatcaacaaa ttagagaaaa acttattaaa catatgaaat
//
LOCUS       DAZGJW010000022        42086 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZGJW010000022 DAZGJW010000000
VERSION     DAZGJW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.022"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            230..856
                     /locus_tag="ACHKIK_001270"
     CDS             230..856
                     /locus_tag="ACHKIK_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO9082481.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            877..2979
                     /locus_tag="ACHKIK_001271"
     CDS             877..2979
                     /locus_tag="ACHKIK_001271"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO9082482.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            2992..3630
                     /locus_tag="ACHKIK_001272"
     CDS             2992..3630
                     /locus_tag="ACHKIK_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO9082483.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3694..4362
                     /locus_tag="ACHKIK_001273"
     CDS             3694..4362
                     /locus_tag="ACHKIK_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO9082484.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4359..5144
                     /gene="phsC"
                     /locus_tag="ACHKIK_001274"
     CDS             4359..5144
                     /gene="phsC"
                     /locus_tag="ACHKIK_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO9082485.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5148..5960
                     /gene="ydhT"
                     /locus_tag="ACHKIK_001275"
     CDS             5148..5960
                     /gene="ydhT"
                     /locus_tag="ACHKIK_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO9082486.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(5972..7576)
                     /locus_tag="ACHKIK_001276"
     CDS             complement(5972..7576)
                     /locus_tag="ACHKIK_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO9082487.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDIGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7702..8007)
                     /locus_tag="ACHKIK_001277"
     CDS             complement(7702..8007)
                     /locus_tag="ACHKIK_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO9082488.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8115..8191)
                     /locus_tag="ACHKIK_001278"
     tRNA            complement(8115..8191)
                     /locus_tag="ACHKIK_001278"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8155..8157),aa:Val,seq:gac)
     gene            complement(8196..8272)
                     /locus_tag="ACHKIK_001279"
     tRNA            complement(8196..8272)
                     /locus_tag="ACHKIK_001279"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8236..8238),aa:Val,seq:gac)
     gene            8580..9836
                     /locus_tag="ACHKIK_001280"
     CDS             8580..9836
                     /locus_tag="ACHKIK_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO9082489.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEERA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9877..11250)
                     /gene="mdtK"
                     /locus_tag="ACHKIK_001281"
     CDS             complement(9877..11250)
                     /gene="mdtK"
                     /locus_tag="ACHKIK_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO9082490.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            11465..12106
                     /gene="ribE"
                     /locus_tag="ACHKIK_001282"
     CDS             11465..12106
                     /gene="ribE"
                     /locus_tag="ACHKIK_001282"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO9082491.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12146..13294)
                     /gene="cfa"
                     /locus_tag="ACHKIK_001283"
     CDS             complement(12146..13294)
                     /gene="cfa"
                     /locus_tag="ACHKIK_001283"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO9082492.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13585..14796)
                     /gene="punC"
                     /locus_tag="ACHKIK_001284"
     CDS             complement(13585..14796)
                     /gene="punC"
                     /locus_tag="ACHKIK_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO9082493.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14909..15841
                     /gene="punR"
                     /locus_tag="ACHKIK_001285"
     CDS             14909..15841
                     /gene="punR"
                     /locus_tag="ACHKIK_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO9082494.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15838..16863)
                     /gene="purR"
                     /locus_tag="ACHKIK_001286"
     CDS             complement(15838..16863)
                     /gene="purR"
                     /locus_tag="ACHKIK_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO9082495.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17162..17251
                     /gene="cydH"
                     /locus_tag="ACHKIK_001287"
                     /gene_synonym="ynhF"
     CDS             17162..17251
                     /gene="cydH"
                     /locus_tag="ACHKIK_001287"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO9082496.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17417..18586
                     /locus_tag="ACHKIK_001288"
     CDS             17417..18586
                     /locus_tag="ACHKIK_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9082497.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18732..19313)
                     /gene="sodB"
                     /locus_tag="ACHKIK_001289"
     CDS             complement(18732..19313)
                     /gene="sodB"
                     /locus_tag="ACHKIK_001289"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO9082498.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19441..20268)
                     /gene="mepH"
                     /locus_tag="ACHKIK_001290"
     CDS             complement(19441..20268)
                     /gene="mepH"
                     /locus_tag="ACHKIK_001290"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO9082499.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20603..20950
                     /gene="grxD"
                     /locus_tag="ACHKIK_001291"
     CDS             20603..20950
                     /gene="grxD"
                     /locus_tag="ACHKIK_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO9082500.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21001..25617)
                     /locus_tag="ACHKIK_001292"
     CDS             complement(21001..25617)
                     /locus_tag="ACHKIK_001292"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO9082501.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQECSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDTAHFE
                     STSGATSNHVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVSDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECTARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIIAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25710..26357)
                     /gene="rnt"
                     /locus_tag="ACHKIK_001293"
     CDS             complement(25710..26357)
                     /gene="rnt"
                     /locus_tag="ACHKIK_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO9082502.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26460..26867)
                     /gene="gloA"
                     /locus_tag="ACHKIK_001294"
     CDS             complement(26460..26867)
                     /gene="gloA"
                     /locus_tag="ACHKIK_001294"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO9082503.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26948..28045)
                     /gene="nemA"
                     /locus_tag="ACHKIK_001295"
     CDS             complement(26948..28045)
                     /gene="nemA"
                     /locus_tag="ACHKIK_001295"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO9082504.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGANEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28082..28681)
                     /gene="nemR"
                     /locus_tag="ACHKIK_001296"
     CDS             complement(28082..28681)
                     /gene="nemR"
                     /locus_tag="ACHKIK_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO9082505.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            28784..29023
                     /locus_tag="ACHKIK_001297"
     CDS             28784..29023
                     /locus_tag="ACHKIK_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO9082506.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29072..29968
                     /locus_tag="ACHKIK_001298"
     CDS             29072..29968
                     /locus_tag="ACHKIK_001298"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO9082507.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQQLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30049..30570
                     /gene="sodC"
                     /locus_tag="ACHKIK_001299"
     CDS             30049..30570
                     /gene="sodC"
                     /locus_tag="ACHKIK_001299"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO9082508.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30571..32583)
                     /locus_tag="ACHKIK_001300"
                     /pseudo
     CDS             complement(30571..32583)
                     /locus_tag="ACHKIK_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(32583..33440)
                     /locus_tag="ACHKIK_001301"
     CDS             complement(32583..33440)
                     /locus_tag="ACHKIK_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO9082509.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33443..33679)
                     /locus_tag="ACHKIK_001302"
     CDS             complement(33443..33679)
                     /locus_tag="ACHKIK_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO9082510.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33880..34314
                     /gene="slyA"
                     /locus_tag="ACHKIK_001303"
     CDS             33880..34314
                     /gene="slyA"
                     /locus_tag="ACHKIK_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO9082511.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34361..34828)
                     /gene="slyB"
                     /locus_tag="ACHKIK_001304"
     CDS             complement(34361..34828)
                     /gene="slyB"
                     /locus_tag="ACHKIK_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO9082512.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35102..36211
                     /gene="anmK"
                     /locus_tag="ACHKIK_001305"
     CDS             35102..36211
                     /gene="anmK"
                     /locus_tag="ACHKIK_001305"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO9082513.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVEPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36309..36638
                     /gene="mliC"
                     /locus_tag="ACHKIK_001306"
     CDS             36309..36638
                     /gene="mliC"
                     /locus_tag="ACHKIK_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO9082514.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36697..37353
                     /gene="pdxH"
                     /locus_tag="ACHKIK_001307"
     CDS             36697..37353
                     /gene="pdxH"
                     /locus_tag="ACHKIK_001307"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO9082515.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37482..38756
                     /gene="tyrS"
                     /locus_tag="ACHKIK_001308"
     CDS             37482..38756
                     /gene="tyrS"
                     /locus_tag="ACHKIK_001308"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO9082516.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38815..39678
                     /gene="pdxY"
                     /locus_tag="ACHKIK_001309"
     CDS             38815..39678
                     /gene="pdxY"
                     /locus_tag="ACHKIK_001309"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO9082517.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39722..40327)
                     /gene="gstA"
                     /locus_tag="ACHKIK_001310"
     CDS             complement(39722..40327)
                     /gene="gstA"
                     /locus_tag="ACHKIK_001310"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO9082518.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40433..41935)
                     /gene="dtpA"
                     /locus_tag="ACHKIK_001311"
     CDS             complement(40433..41935)
                     /gene="dtpA"
                     /locus_tag="ACHKIK_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO9082519.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT        10607 a        10382 c        11009 g        10088 t
ORIGIN      
        1 ctttgtaatt ttaattgata acgatcaatg ttaagggctc agaataatca cattattttt
       61 tctcctaacc ataaaggatt agtttatcgg cgttaaatct tttctcatac actatcgggt
      121 tagggaaaat atctggtgtt ggggacagag tcagcagcgt attagttgaa tgcttaatat
      181 caggctgctg aattgtcagc actgtgtttg cgaagaggat gcgaaataaa tgaacccggt
      241 tgatcgtcca ctattagata ttggcttaac gcgattagaa ttcttgcgta tatcaggaaa
      301 aggccttgct ggtctaacaa ttgcccctgc gttactgtca cttttgggtt gcaaacagga
      361 agatattgat agcggcacag tagggttgat aaatacaccc aaaggggtgc tggtgaccca
      421 gcgagcgcga tgcacgggtt gccaccgctg tgaaatctct tgtaccaact tcaacgatgg
      481 ctcagtaggg acattcttct cccgtatcaa aatccaccgc aattatttct ttggcgacaa
      541 cggggttggc tctggcggcg gcctgtatgg cgatctcaac tataccgcgg acacctgccg
      601 tcaatgcaaa gagccgcaat gcatgaacgt ctgcccgatt ggtgcgatta cctggcagca
      661 gaaagaaggc tgtattaccg tcgatcataa acgctgtatt ggctgtagcg cctgtactac
      721 ggcatgcccg tggatgatgg ccaccgtaaa taccgaaagt aaaaaatcct cgaaatgtgt
      781 gttatgcggt gaatgcgcaa acgcctgccc aacaggggcg ttaaaaatta tcgagtggaa
      841 agatattact gtgtgaatct tgcaaaggaa aaaatcatgg ctaacggttg gacaggtaat
      901 atattaagag tcaatctcac gacaggaaat attaccctcg aagattccag taagtttaaa
      961 agttttgtcg gtggcatggg cttcggctac aaaattatgt atgacgaagt accgccaggc
     1021 acgaaacctt tcgatgaagc gaataaatta gtctttgcta ccggcccatt aactggatct
     1081 ggtgccccct gtagttctcg cgtaaatatc acctcacttt ctacttttac caaaggaaat
     1141 ttagttgtcg atgcccatat gggtggcttt tttgcagcgc aaatgaaatt cgctggatac
     1201 gacgtcatta ttatcgaagg gaaggcgaaa tcaccggtat ggctgaagat taaagatgac
     1261 aaagttagcc tggaaaaagc cgatttctta tggggaaaag ggacgcgcgc aacgacggaa
     1321 gaaatttgtc gattgaccag tccggaaacc tgtgtggcgg ctattggtca ggctggggaa
     1381 aaccttgttc ctctctctgg catgttgaat agccgtaacc acagcggcgg tgcgggaact
     1441 ggcgcaatta tgggttcgaa aaacctgaaa gcgattgcgg ttgaagggac gaaaggggtc
     1501 aacattgccg atcgtcagga gatgaagcgt ctcaacgatt acatgatgac tgaacttatt
     1561 ggtgcgaata acaaccatgt cgtgccaagt acgccgcaat cgtgggcaga gtattcagat
     1621 cccaagtcac gttggacagc acgtaaaggg ctgttttggg gcgcggctga aggtggtccg
     1681 attgaaacgg gggaaattcc gccaggcaat cagaacacgg tcggctttcg tacctataaa
     1741 tccgtttttg acttaggtcc ggcggcagag aaatacacag taaaaatgag tggctgccac
     1801 tcttgcccga tccgttgtat gacccaaatg aatattcctc gggtgaaaga gtttggcgtg
     1861 cccagcacag gtggtaacac ttgtgtagct aactttgtcc ataccaccat cttcccgaac
     1921 gggccgaaag attttgaaga taaagacgat ggtcgtgtga ttggtaacct ggttggtctg
     1981 aatttgttcg atgactacgg cctatggtgt aactacgggc agctgcatcg cgactttact
     2041 tattgttaca gcaaaggggt gttcaagcgt gttctgccag ctgaagagta tgcggaaatt
     2101 cactgggatc aactggaagc gggtgacgtt aatttcatta aagattttta ctaccgtctg
     2161 gcgcatcgtg tgggtgagct gagtcacctg gctgatggtt catatgccat cgcagaacgc
     2221 tggaatttgg gtgaagagta ctggggctac gcaaaaaata aactctggtc gccgtttggc
     2281 tatccggttc accatgccaa tgaagcgtca gcgcaggtcg gttccattgt taactgtatg
     2341 ttcaaccgtg actgcatgac gcatacccat atcaatttta ttggttcggg tttgccattg
     2401 aaactgcagc gtgaagtggc gaaagagctt tttggttctg aagatgccta cgatgaaacc
     2461 aaaaactaca cgccaatcaa tgacgcaaaa attaaatatg caaagtggtc gctgttgcgg
     2521 gtctgtttgc ataacgccgt caccctgtgc aactgggtct ggccaatgac cgtttcgccg
     2581 ctgaaaagcc gtaattaccg gggcgatctg gcgcttgaag ccaaattctt caaagcgatc
     2641 accggcgaag agatgactca ggaaaaatta gatttagctg cagagcgtat ctttacgttg
     2701 catcgtgcct acacggtaaa actgatgcaa accaaagata tgcgtaatga acacgatctt
     2761 atctgttcct gggtattcga caaggatccg cagatcccgg tctttactga aggtactgac
     2821 aaaatggatc gtgacgatat gcatgcttcg ctcacgatgt tctacaaaga aatgggctgg
     2881 gacccacagc ttggttgtcc gacccgcgaa acattgcagc gtctggggct ggaagatatt
     2941 gccgccgatc tggcagcaca caatctactg cctgtgtaag gaatgggtga gatgaaccat
     3001 cgggatgaat tgccgttagc gaaagtcagc gaagttgacg aggcgaagcg gcaatggtta
     3061 caggggatgc gtcaccccgt tgatacggta accgaaccag aaccggctga aatcctggca
     3121 gagttcattc gccagcattc tgcggctggg cagctggtgg cgcgcgccgt gtttctctcg
     3181 ccaccctatt cggttgcaga agaggagctg tcagttttgc tggaaagcat taagcagaac
     3241 ggagactacg cggatatcgc ttgtatgacg ggaagccagg atgattacta ttactcaacg
     3301 caggccatga gtgaaaacta cgcggcgatg tctttgcaag ttgtggagca ggacatttgt
     3361 cgcgccattg cacacgccgt tcgttttgag tgccagacat atcctcgtcc ttacaaagtc
     3421 gccatgctga tgcaagcgcc atattacttc caggaagcgc agattgaagc cgcgatcgcc
     3481 gcgatggatg ttgcacctga atatgccgat atacggcagg tggagtcatc gacggcggtg
     3541 ttgtacttgt ttagcgagcg atttatgacc tatggcaagg cttatggctt atgcgaatgg
     3601 ttcgaggtag agcagtttca gaacccctga aatttcaggg agatgtcatg gatagggtgg
     3661 acagttgcag ccactgcatt aggggataat cagatgtcct tcactcgacg caaatttgtt
     3721 ctggggatgg gaacagtaat cttttttact ggttctgcgt cttctctgtt agcgaacacg
     3781 aggcaagaaa aggaagttcg gtatgccatg attcatgacg agtcacggtg caatggctgt
     3841 aatatttgcg cccgcgcatg ccgtaaaact aaccacgtcc ccgctcaggg aagccgttta
     3901 tcgatagcac acattccagt caccgataac gacaacgaaa cgcagtatca cttttttcgc
     3961 cagtcatgtc agcactgcga agatgctccg tgcatcgacg tatgcccgac aggggcgtcg
     4021 tggcgtgatg agcaggggat cgtgcgggta gaaaaatcgc agtgcatcgg ctgcagttat
     4081 tgcattggcg cttgtccgta ccaggttcgc taccttaacc ctgtcactaa agtggcagat
     4141 aaatgtgatt tctgcgctga gtcccgactg gcaaaaggct ttccgcccat ttgcgtcagc
     4201 gcctgcccgg aacacgcatt gatctttggt cgcgaagata gcccagaaat tcaggcgtgg
     4261 ttgcagcaaa ataaatacta tcaatatcag ctacctggtg ccgggaaacc gcatctgtat
     4321 cgccggttcg gtcaacattt gattaaaaag gaaaatgtat gaacccgtcg caacatgctg
     4381 aacagtttca gagccagtta gcgaactatg tgccacagtt cactcccgaa ttttggccgg
     4441 tgtggttgat cattgccgga gtattgctgg ttgggatgtg gttggtgctg gggctgcatg
     4501 ccttgcttcg tgctcgtggc gtgaagaaat cagtcaccga ttatggtgag aagatttatc
     4561 tttactgcaa agcggtcaga ttatggcact ggtcgaatgc gttactcttt gtattgttgc
     4621 tggcaagtgg gctgataaat cactttgcgc tggtgggcgc aactgcggtt aaaagtctgg
     4681 ttgcggtgca tgaagtttgc ggatttttgt tactcgcatg ctggctcggc tttgtgctga
     4741 tcaatgccgt tgggggtaat ggtcaccact atcgcattcg tcgtcagggg tggctggagc
     4801 gagcggcaaa acaaacgcga ttttatttgt ttggcattat gcagggggaa gaacatcctt
     4861 tcccggcaac aacccagtct aaatttaatc ccttacagca ggtcgcctat gttggtgtta
     4921 tgtatggatt gctgccgttg ttactattga cggggctgct gtgtctctat ccgcaagccg
     4981 tgggagatgt gtttcctggc gtaagatact ggttattgca ggcgcatttt gctctggcat
     5041 ttataagcct cttttttatc ttcggtcatc tttatctttg caccacgggg cgtacgccac
     5101 acgaaacctt taaaagcatg gtcgatggct atcaccggca ctaacgaatg atcattacac
     5161 gagcagacct gcgagaatgg cgtatcggag cggtgatgta tcgctggttc ctgcgccatt
     5221 tcccacgcgg cggtagttat gccgatattc atcatgcact gattgaggaa ggatatacgg
     5281 actgggcgga gagtctggtt gagtatgcgt ggaaaaaatg gctggcggat gaaaacttcg
     5341 cgcatcagga agtttcctct atgcaaaaac tggccacaga ccccggagag ataccatttt
     5401 gcagtcagtt tgcccgcagc gacgatcatg ctcgcatcgg ctgttgtgag gataacgccc
     5461 gaatcgcaac cgctgggtat gccgcgcaaa ttgccagtat gggttatagc gtgcggattg
     5521 gaagtgtcgg ctttaacagt cacattggca gctccggtga acgcgcacgg gttgctgtaa
     5581 ccggtaattc ttcgcggata agcagcgctg gagatagtag tcggattgcg aataccggaa
     5641 tgcgagtacg cgtttgtaca ttgggtgaac ggtgtcatgt cgccagtaat ggtgacctgg
     5701 tgcaaatcgc cagctttggc gcaaacgcca gaattgccaa cagcggcgac aatgtacaca
     5761 tcattgccag cggtgaaaat tcaacggtag tgagcacggg cgtggtcgac tcaattattc
     5821 tcgggccagg gggaagcgcc gcactggcat atcatgacgg cgaaagagtt cgcttcgccg
     5881 tcgccattga aggtgaaaac aatattcgaa ccggagtccg ctatcgtctt aatgaacagc
     5941 atcagtttgt tgagtgctga tagctttacc tttaatcaaa cgaaagacgc cgacgagcac
     6001 gggatgcagg cttcacgacc gcccgagcca tcgcctcacc aatttctgca catgccgtaa
     6061 gtccctgaac gaaaggctgg tcgtgcatca accagggcaa cgcgccgaac gctacgcgtc
     6121 cacgaatatc ttcgggttgc tgtaacgtat aatcttcgcc aatatcaggg atttcatccc
     6181 ctgttttctg taattgctcg cgtagcccag ggaaaggaat atctttcact ttaagcggac
     6241 gctgtccgcg ggcatcaata aaaacgtcaa acgagtagct gttgtcttcc gttttcagga
     6301 cggtgcgcga ctcattaatt tccattttgt agtcttcacc gagggcgaga atatgaatga
     6361 ttcccgcttc acgtaaggca agtaggcgac gaatagactc tgaagggatt gccgcataat
     6421 tatcgatgaa aaccctggca aggcctttac tgaaccgttt atggtcgtgt tcattcagat
     6481 gaggaacaat ttcctgtacg gcttcatgca ggcgcagaat gacataacgc cagggaacag
     6541 tatgtttttc tcgtttattg cgttcgactt cctggagatt tttttctgcc cagtcaaatg
     6601 ggtcgcgttg tttgcgctcg gcaaaccagg cttgagcaaa ggaatcgaca ttcaggctct
     6661 ctaaggctat gcgttggctc cagtctggat cagcaaactt gatttcctct actatcaatc
     6721 taaatacccg atccaaaagg ccctcttcac ctttttgaat ctcagcattt aatgcctgat
     6781 cggtgacgat gtgtaagggc tcgtagggaa tagggcaata gaaatcggct tcgggtaaaa
     6841 tacccgtgcg cgacatcaac gtaatattta gcttttcact tgcgttatcg cggttaaaaa
     6901 cgacgtgttg tttatcatct tcaatgaacg aaccatgctg aatagccact gccattgccg
     6961 catccagtcc gctcaaggat gttcccataa tacccacgtt acacgcatcg acctttgctt
     7021 ccatcaggcc tgaccacgga ctgggaaaat acgttctggt tgcttcttct tcatcaggcc
     7081 agacgtgacc cgtggcaatc accgctaaat caaacgtctc gctgggtaaa tcctgatttg
     7141 tagcgagcat gacgccagca tttgtaattt gcagatcggt aacctggcat gattcataaa
     7201 cagccactgc aaatttttgc tgtcgtgcct ggtctactaa tcgtaaaaat tgatcgcgga
     7261 aatattcgcc cagcagaatt cgcggtaaaa actgacgatc gtgcaaggtt tcttttttaa
     7321 cgccataacg ctggagatgg ctggcttctt gcttttgtag ccattcgaga tacgtacaat
     7381 taatcggcgg tatttcaata ctggcaatat ttgccagcat catttttgag ttttcctcat
     7441 cactgtatgg catcccgaca ccggcctcgt cagcctgctc gaagatagaa atagaaagtg
     7501 gagtttgttg ctgtagaagc gagaataagg tgtagatccc cgtaggcccg gcacccacaa
     7561 tagcaatttt tttcattgtt acttacctgt acgcattctt tcgaatttag tgttatcaga
     7621 taagtttggt tgagattttt agaatttgag aaatttcaga caggtatgaa aagggcgcta
     7681 accgcccttg attaaattct gtcaggcgag ttttgcctga ttgatttgac tcagtggctc
     7741 attgacatcg aaaactttgg cgacaacttc ctcaacgccg agatttttaa gtcgggcagt
     7801 atgtttttcc agataggcaa gggcgctttt ttcatcagta aaaagataga tcccaccggc
     7861 ttcgtggttc ttttcacttt ctgtccatac cttccacaga aaaccaggtt cctgattaat
     7921 cgactcagca agtggtttaa gctgctcagc cattgcgtcg ccaaatgggc cattaaaagc
     7981 aaaatgaagt tgtaacaaag ttgccatagc aattctcctt ctgataacgc atacgaagac
     8041 agtgtaaatc ttttaacgta ttggatacag agaagaaaca gaggaaaaaa ggagaagacc
     8101 agattaagaa aatctggtgc gtccgagtgg actcgaacca ccgaccccca ccatgtcaag
     8161 gtggtgctct aaccaactga gctacggacg caggatggtg cgttcaattg gactcgaacc
     8221 aacgaccccc accatgtcaa ggtggtgctc taaccaactg agctatgaac gcaacgttgt
     8281 aggtgacaac ggggacgaat attagcggca gagtgggaag gtggcaagag gcaaaacgta
     8341 attttctgcg ctatttcgac cgtttgcaga gcttttaagc aaattggcta tattttgttg
     8401 atttgcaagg gcgattttta ttcaggatcg catttacatc tgatacaacc caataattcg
     8461 tgtatcagag tttaaacaga gttgtttgcc accatctcaa atctgtcgtg gataaatcct
     8521 ccgtagcctt tacacttagg tagcaataaa ggcacacaga tgcagcaaag gaataaatca
     8581 tgggatctga tgcgaaaaac ttgatgagcg acgggaatgt gcaaattgtt aagaccggcg
     8641 aggtcattgg cgcgacgcaa cttactgaag gcgagttgat tgttgaagct ggcggaagag
     8701 ccgaaaatac cgtggtcacg ggggctggct ggttgaaagt ggcaaccggt gggatcgcca
     8761 aatgcacaca gtacggtaac aatggcacgc tatcggtcag cgacggtgcc attgccacag
     8821 atattgttca gtccgaggga ggcgcaatta gtctctctac gctcgctacg gttaatggcc
     8881 gccatcccga aggtgaattc agcgttgatc agggttatgc ctgcggtttg ttgctggaaa
     8941 atggcggtaa cctgcgtgta ctggaaggac atcgcgcgga aaaaatcatt ctcgatcaag
     9001 agggcggcct gttggtcaat gggacaacct cagctgtcgt ggtagatgaa ggtggtgaat
     9061 tgttggtgta tccaggtggg gaagccagca attgtgaaat taatcagggc ggcgttttta
     9121 tgctggccgg gaaagccagt gatacgttgc ttgctggtgg caccatgaat aatctcggtg
     9181 gtgaagactc tgacactatt gttgagaatg gatccattta tcgtctgggg acggatggcc
     9241 ttcagctcta cagttccggt aagacgcaaa acctgtccgt gaatgtgggt ggtcgggctg
     9301 aagtgcatgc cggtacgctg gaaaatgcgg taatacaagg tggaacagtg atcctgttgt
     9361 cacccaccag cgcggacgaa aattttgtcg tagaggaaga gcgcgcaccg gttgaactga
     9421 ccgggagtgt tgcattactg gacggcgctt caatgattat tggctatggt gcagatctgc
     9481 aacaatcaac gattactgta cagcagggcg gtgtgttgat tctcgacggc agtacggtaa
     9541 aaggtgacag tgtcactttt agtgttggta acatcaatct gaatggcgga aaactgtggc
     9601 tgatcactgg tgcggcaacg catgtgcaac tgaaagtgaa acgcctgcgc ggagagggag
     9661 cgatttgcct gcaaaccagt gcgaaagaaa tctcacctga cttcatcaat gtgaaagggg
     9721 aagttaccgg ggatatacgc gttgagataa cagatgccag tcggcaaact ctgtgtaacg
     9781 cactgaaact acagccagac gaagacggga ttggcgcaac gctccagcct gcgtaaaaac
     9841 acaggcgcaa cccgtgaagg ttgcgccttg tctttattag cgggacgctc gttgcagaat
     9901 gatggctgac ggcagacgtt gcaggaaccg catacgcagc atcatcataa tggctgccga
     9961 cgtcaggcca ataataaagc ctatccagaa gcctgctggc cccataggtt caacgaccag
    10021 atcggtcagt gccagaatat agccgcttgg caagcccagc acccagtaag ccgtaaaggt
    10081 aatatagaaa atggaacgcg tatctttata accacgcaaa atcccactgc caatcacctg
    10141 gatcgagtca gaaatctgat ataccgccgc cagcaacatc aaatgcgcag ccagcgttac
    10201 aacctcggga ttgtcgttgt acaacagggc gatttgctcc cgcagtgaaa ccgtgaatat
    10261 ggccgtcagg gttgccatac agacacccac cataagcccg gtccgcgcag cggtttgcgc
    10321 atccagcgtt gagccctgac ccagacgata acctacgcgg atagttaccg ctgccgccag
    10381 cgacatggga agcacgaaca ttagtgaact aaagttcagg gcaatctggt gtcctgcgac
    10441 atcaacaata ccgagcggag acactaacag agccacgaca gcaaacagtg tcacttcaaa
    10501 gaacagtgcc agcgcaatcg gcaaaccgag ttgaatcagt cgtttcataa ccgcaggatc
    10561 gggtttcgcg gtgccttttt cgttacgaat atcgcgcatg gagcgggcgc gtttaatgta
    10621 agaaaccatg gcaaggaaca taacccagta caccgccgca gtagccacgc cacaaccaac
    10681 gccaccgagc tcaggcatac cgaaatgacc ataaataaag atatagttca ccggaatgtt
    10741 caccagcagg ccgataaagc ccattaccat acccggcttg gtttttgcca gaccttcaca
    10801 ctggttacgg gcaacctgga agaacagata gcccggcgcg ccccacaaca acgcacgcag
    10861 ataacccacg gctttgtccg ccagagccgg atcgatgttt tccatggagc ggataatgta
    10921 acctgcattc cacagcacca gcataatgag aacggaaaca aaacctgcca gccagaaacc
    10981 ttgtcgcacc tgatgcgcaa tgcgctcacg tcgaccggaa ccatttaatt gcgcgataac
    11041 cggcgttaat gccagcagca gtccgtgacc aaagaggatc gccggaagcc agatagaagt
    11101 accgatagcg accgccgcca tgtcggtggc actatagccg cccgccatca cggtatcgac
    11161 aaaacccatc gcagtttggg cgatttgcgc gagaatcacc gggattgcta atgctaataa
    11221 cagacgcgct tcactgatat acttctgcac gtgaacacct tttatttgta gttatatgag
    11281 agactaaaaa gccgcctgaa tgggcagcaa aaagaagaag caggggaaat tcagtctatt
    11341 gtagcgagga tttattaatt ctccagtgaa aaaatagtca ttcttacccc ttcactggta
    11401 agggcgtttg ccacctgcta ttgtgctgaa cagaatgtct taactgattt caggagttgt
    11461 aagtatgttt acggggattg tacagggcac cgcaaaactg gtgtcgattg acgagaaacc
    11521 aaattttcgt acgcatgtgg tggagttacc cgaccacatg ctggacggcc tggaaaccgg
    11581 tgcgtccgtg gcgcataacg gttgctgcct gaccgtgacg gaaattaacg gcaaccatgt
    11641 cagttttgac ctgatgaaag aaacgttacg cattaccaat cttggcgatt taaaagtggg
    11701 ggattgggta aacgttgagc gtgcggcgaa attcagtgat gaaattggcg gacacttaat
    11761 gtcaggtcat attatgacca ctgctgaagt ggcgaaaata ttaacctcag aaaataatcg
    11821 ccagatctgg tttaaagtcc aggatagtca gttgatgaaa tatattctgt acaaaggatt
    11881 tattggcatc gacggtatta gcctgaccgt cggcgaagtc acgccaacgc gtttttgcgt
    11941 ccatttaatt ccggaaacac tggaacgcac gactcttggg aagaaaaaac tcggcgcacg
    12001 cgtcaacatt gaaattgatc cacaaactca ggcagtggta gatacggtag aacgtgtgct
    12061 ggcggcacga gaaaatgcca tgaatcaacc aggtacagaa gcctgatctg aactcgcccc
    12121 cgaagagggg gcgatagaat agcctttagc gagcgactcg aaggccgttt tcaacaccgc
    12181 gtgagaacac cacctgccag agctgaatat cacgggcgcg gaaagcacct gcacaagcat
    12241 tcagataata ggtaaacatt cgtttaaagc gttcactgta gttatctgca atttctggcc
    12301 atgcggcgag gaatcgttca taccacgcca tcaacgtagt atcgtaatca gcaccgaagt
    12361 tatgccagtc ttccatcaca aagtggggtt cgctggactg agcaatctgg cgtacagagg
    12421 gcaggcaacc gttcggaaaa atatatttat taatccaggg atcaacattc agatcggttt
    12481 ttttcgaacc gatagtatgg agcaggaata taccttccgg tttcaaatta cgatccacca
    12541 ccgcaaaata ggtatcgtaa tttttcggtc cgacgtgctc gaacatcccc acagaaacaa
    12601 tacgatcaaa ctggtcgttc aggtcacgat aatcttgcag caaaatggtg acatccaggc
    12661 cttcacagcg ttcctgagcc attttttgct gttcggcaga aatggtgacg ccgaccacgc
    12721 ttacgtcata gttagatgcc atgtagtgcg ccagtccgcc ccagccgcag ccaatatcca
    12781 gtacgcgcat ccctggtttt aactgcaatt tttcacaaat cattttgagc ttcgcctgct
    12841 gggcagattc cagattatcg gcatctttcc agtaagcgca ggaatattgc atgaagggat
    12901 caagcatgcg actgaacaag tcattaccca aatcgtaatg ctctttgccg actatccagg
    12961 cacgtttttt actctgcaga ttgaagagac gggcgcctgc aatacgcagc gtgtctttga
    13021 aatgatgggg gagttggttc tcgagacctg cgcgtaagac tttgctaaaa aacatatcca
    13081 gtcggtcaca ttcccaccag ccatccatat aactttcgcc taaccccaaa gagccttctt
    13141 gcagaacgcg tttaaaaaaa tcggggtttt tcacacgaat atccgccggg gcagaaccgt
    13201 taatggctat accggcacgg ctaagtaatt cgttggcgat acggtaccag ttgtcatccg
    13261 gtacactgac ttcttctata cacgatgaac tcatagtttc tccatcactg gtcggtgatc
    13321 agaaccttaa aacagcgtag acgctttttg ggctttgtga gaaatctcgc agaaaaaacc
    13381 gcgagtctat aatccgacgt agaacagagg gaaaggagga acccttgccg aaatgaccat
    13441 ccatagtgag tcgggaacga tcctgtcccc gtaaaaacat aatatttatc gtagctttaa
    13501 caactcgaaa ttagtatagg ccgctaattt caatcataca acaaaatttt gttagcagcc
    13561 taagtataag tatatcgata tagatcagtg tgattcgcta tgagcgactt cggcattgcc
    13621 atgattctgg cagccaactt cttcacaacg ttgcatcatg taacccagcg cgaccagcac
    13681 tactgttgat aacatcacgc tggtggtggt gagcaatggc gtgctgatac tgatcagcca
    13741 ggaaactacc agacttgcga ggaagcacag acccagttgc agagtgttct gcaacgctgc
    13801 ggcgcgaccg gttgcatgtg ggaagggacg cagcgcctgg gcgacaacaa tagggtagat
    13861 cgcgccattg gcaatcgcca tcacacagaa tgggatcagg atttcgacca gcgacacatg
    13921 gctaatgaag cccgcagccc aggtcgcaat gacgctgaca gcaaacagca ccagcaacca
    13981 cggtaataac tgcttgcctt gccatttctg cagtgcggcg cgacagccat aaccaccaat
    14041 cagaaacgcg atagtttgcg ggacataact taaaccaata actgccgggc tgtagcccat
    14101 ttcactaagg atgaacggtg aaccggtcag ccatgcaaaa aaactggctg aacaggctgc
    14161 atatatcagc acgttgccgc gataggtttt agaacgtagc aggtcggtaa aggtcagacc
    14221 atcctgacta ttgttacggg ccttcgtcgt gggtttgagc cagaaaatag gcagaatcag
    14281 caccacggta atggcaaaca gggtggcgaa aatcgcctgc caggaaaaat ggaccagcag
    14341 ccagcttcct aacaggggag ccagtgccgg agacagaccc accagcggca tgatggtcgc
    14401 aaaaatacgg ttgactttct gtgaaggata ataatctgtc actaatgctt gccagataac
    14461 cgccgcggcg cagaccccca cagcctgtac aaaacgcaat accagcagcg tagcggcgtt
    14521 ttctacccac agcatcccca gactacctaa cgcaaaaatt gtcaggccga ttaataacac
    14581 cggtttacga ccataacggt cggagagtgg cccccacaga agctgggctg cggcaaaacc
    14641 ggcaaggaac agactaaggc tggcactgac agcagacgca ggcgtttgca ggtcggcctg
    14701 tatggcggcg aaagcaggca gatacatatc ggttgccaga aaaccgagta cgctcaaacc
    14761 cgccagccag actaaaaatc ttttcccagg ttgcatgtat attctctaaa tctctcaatt
    14821 aaacaccgcc gcaaagtgta gggagtgaaa ttagtgttgt gaaacggtaa tatttgatga
    14881 ctggtttcaa aaattttgca ggcagactat gtggtcagaa tattcactcg aagttgttga
    14941 tgcggtagcg cgtaatggta gttttagcgc tgcggcacag gagctgcatc gcgttccttc
    15001 tgcggtcagc tataccgtgc gtcagctgga agagtggctg gcggtaccgc tctttgaacg
    15061 acggcaccgt gatgtggagc tgaccgctgc tggagcgtgg tttctcaaag aagggcgctc
    15121 tgttgtcaaa aaaatgcaga tcacccgcca gcaatgtcag cagatagcga acggctggcg
    15181 cggtcagtta gctatcgcag tggataatat tgtcaggcca gaacgtacac ggcagatgat
    15241 cgttgatttt tatcgccatt ttgacgacgt cgaacttctg gtctttcagg aagtgtttaa
    15301 cggtgtctgg gatgcgcttt ccgatgggcg cgtggaactg gcgattggcg caacacgcgc
    15361 gattccggta ggcggtcgtt atgccttccg ggatatgggg atgctaagct ggagttgcgt
    15421 tgttgccagc caccacccgc tggcgttgat ggatggcccg ttcagcgatg atacgttgcg
    15481 caactggccg tcgttggtgc gcgaagacac ctcgcgaacg ctacctaaac gtattacctg
    15541 gttgctggat aaccaaaaaa gagttgtcgt gcctgactgg gaatcatcgg caacctgtat
    15601 ctcggcagga ttatgcatag ggatggtgcc aacacatttc gccaaaccgt ggcttaatga
    15661 ggggaagtgg gtggcattag aactggaaaa cccctttccg gattcggcat gttgtctgac
    15721 atggcagcaa aatgatatgt cgcctgcgtt aacctggctg cttgagtatc tcggcgatag
    15781 tgagacgctg aataaggagt ggctgcggga gccggaagag actcccgcaa cgggtgatta
    15841 acgacgatag tcgcggaacg ggccgtcagc cacggagcgg cgttcaatca agcgcggatg
    15901 cacttcaatg gactgcggtt cttcacgttt gttgacgata cgatccaaca gcatgttgaa
    15961 cgctgtttca cccagcgaat cttttggctg gtggatcgtg gtcagcgccg gcgtaaaata
    16021 gcgggcgttg cgcacgttat cataaccgat cagcgaaaca tcctgcggga cgcgcagacc
    16081 catttcatca gcagcacaaa gtgcgcccat tgccatgata tcgccaccac agaagacggc
    16141 agtagggcga tgcggctgcg acagtatttg ctgcatggcg cgataaccag attcgggttc
    16201 aaagtcaccc tgaacaatcc agctttccgg caccttgatc atcgcttctt ccatcgcctt
    16261 cataaaaccg gcaaggcggc ctgcgccggt gttacgttcc agcgggccgg ggatgacgcc
    16321 gatttcgcgg tgaccgcgtt caatcagata acgcccggcc atgtagccgc cttcgaacgc
    16381 gttatcaatg accgcatcgg tgaagtcagc ttttgcttca ccccagtcca tcaccaccat
    16441 tgggatatgg cgatactctt ccagcatcgc cagcaacggc tctgggtact cagaacacat
    16501 caccagcaga ccatcgacgc gtttttgcgc catcatcgac agataagccc gctgtttctc
    16561 aagattgttc cacgcattac ccagaatcag ggtgtaacct ttctggaagc aatttttttc
    16621 aaccgcttca atgatctcgg caaaataggc cgcttcgctg ctggtcgcca gcaaaccgat
    16681 agacttggtg tggttaacct tcaggctacg cgccaccgcg ctaggggagt agtgtaattc
    16741 tttaatcgct gcccacacgg cgttgcgcgt ttcttcagcg acgaaacgtg ttttgttgat
    16801 tacgtgtgac acagttgtag tggaaacgtt tgctcgtttc gctacatctt ttattgttgc
    16861 cattccattt cactccagac cctaacttca gctcctgcaa aatcgcaagg taaacgtttg
    16921 cctttacaca ccttacgcac aacaaaagtg ttgcggtacg ccggaaaaac gacacggagc
    16981 gtcaggaagg ggtcaatggc cagtacgcaa atagtgtaag cgtggaattt tggccgatct
    17041 tgacgaaaag gggaagtggt aaaaacactt atcattataa atgacgggag attttttcat
    17101 cacagtgtgt aaaaatgcgt aatatcacta tttgtgtggt aataaaaaag gagaaaactt
    17161 gatgagcacc gaccttaaat tttcactggt aacaacgatt atcgtcctcg gtttgatcgt
    17221 ggccgtgggt ttgactgccg cgctgcactg atttcctctc gagggagcgc gttctccctc
    17281 gcatctcctt cctgtttgtt agcaaaatgg caaaagtgtt ttctcttttt gttattttca
    17341 tttttttgtg attgctgtca tgctttcgcc actttaatga atatcgcgta atgacgcgaa
    17401 gaggtggaga taaggcatga aaattaacta tccgttgctg gcgctggcga ttggcgcgtt
    17461 tggtatcggg acaacggagt tctctccaat gggtttgttg cccgtcattg cgcgcggtgt
    17521 ggatgtctcg attcccgctg ccggaatgtt aatcagtgcc tatgcagttg gcgtaatggt
    17581 tggcgcgccg ctgatgacgc ttctactttc tcatcgtgcc cgccgcagtg cgttgatttt
    17641 cctgatggca attttcacgc tcggcaacgt actttccgcc atcgcgccgg attatatgac
    17701 cctgatgctt tcacgcattt tgaccagcct gaatcacgga gcattttttg gtttgggttc
    17761 agtcgtggcc gcaagcgtgg tgccaaaaca taaacaggcc agcgcagttg ccactatgtt
    17821 tatggggtta accctggcaa atatcggtgg cgtgccggcg gcgacctggt tgggtgaaac
    17881 catcggctgg cggatgtcat ttctggcaac ggcggggctg ggagtgattt caatggtaag
    17941 tctgttcttc tcattaccta aaggtggtgc aggggcacga cctgaagtga aaaaagagct
    18001 ggcggtatta atgcgtccgc aggtgctgtc tgcattgctg acgacggtac tgggagctgg
    18061 tgcaatgttt actctctaca cctatatctc tccggtacta caaagtatta cccacgcaac
    18121 accagtgttc gtcacggcaa tgctggtgct gattggtgtc ggattctcta tcggtaacta
    18181 tctcggcggc aaactggcag atcgttcagt taacggcacg ttgaaaggct ttttgttgtt
    18241 gttgatggtg attatgctgg caatcccgtt cctggcccgc aatgagttcg gcgcagctat
    18301 tagcatggtg gtgtggggcg cagcaacctt tgcggtcgta ccgccgttac agatgcgcgt
    18361 gatgcgtgtc gccagtgaag cgccaggtct gtcttcatca gtcaatattg gtgcctttaa
    18421 tcttggaaat gcgctgggag cagctgctgg tggtgcggta atttccgctg ggctgggata
    18481 cagctttgtg ccggtgatgg gagcgattgt cgcgggactg gcattattgc tggtgtttat
    18541 gtcagccaga aaacaacctg aaacagtttg cgttgctaac agctaattaa acaaaaacgc
    18601 agaaggataa ccttctgcgt tacagttgct gacaacgtgc gatttatctt gccgaatgcg
    18661 ctgttaacgc ctcatccgcg ttggcatggt aagcgaagcg catcaggcaa tgctgcattt
    18721 gccatcagtt attatgcagc gagatttttc gctacgaatt cccagttcac cagcgcccag
    18781 aagtgctcca gatagccagg acgtgcattg cgatagtcga tgtaataagc gtgttcccag
    18841 acatcaacgg tcagcagcgg agtcgcatcg gtggtcagcg gagtacccgc gttagaggtt
    18901 gaaacgatag ccagtttgcc atcgctgttt ttcaccagcc aggtccagcc agaaccaaag
    18961 tttttgatcg ctgcatcagt aaactgcgct ttgaaatcgg caaagctacc aaaagatgcg
    19021 gcgatagctt cagcgacttt tccagtcggt tcgccaccgg cgttcggtgc caggcagttc
    19081 cagtagaaag tatggttcca gacctgagct gcgttgttga atacgccacc ttcagagctg
    19141 cgaataatct cttccagtga tttaccttca aacgcggtac ctttaatcag gttgttcagg
    19201 ttagtgacat aagtctgatg atgcttgccg tagtgatact cgatagtttc cgcagaaatg
    19261 tgcggtgcca gagcatcttt agcatatggt agtgcaggta attcgaatga cattgctact
    19321 ctcctttatt attaatttgc atacgtacaa tagccttatt gtgcgtatgg atagggtagc
    19381 aaattaaaag gtgagacaaa agatgaactt accctgttgc cgtaacaaca gggtaaagtt
    19441 ttagcgaagt gtttttgggg tcattacccg acgagcgcca acatagtggc gctgccagta
    19501 gtcttcactg agagaagtga tttgaatttc ctgacctgtg cgcggtgact gaataaattt
    19561 gccgttgccg acatacacgc cgacatgatc ggctgtgccg cgtccctgag tacggaaaaa
    19621 gaccaggtcg ccgtttttca gttcactacg ttcgattggc gctgcatcac gcaggtgata
    19681 catttcattc gccgtacgcg gaatacgaat tttcaccaaa tctttataag cgtaataaac
    19741 caggccgctg caatcaaaac cggtacgcgg tgagctgcca ccccaacgat atggcttacc
    19801 aatttgctgc atcagtttat tcattgccac tttcgtagct ttttgcacct tcgctttgtg
    19861 cgcgtcggca agagttcctg acgcggcatt tttgactttc acacaatgcg atttatagcc
    19921 cttacgtttg gtacactttt cggtaaagct ggctgaggcc gttttattga cggcgtgttt
    19981 gctgcgagtt ttggcgtttt tagaagcggt aatggaagat tttttaacgg aagaagcggt
    20041 tttgcttttg gtcgtacttt tactggcggc ttttttcgct gttttctggc tggctttttg
    20101 ggtctttttg gtggttgccg tgcttttctt tttatctgct tttttggtga tatgagtggt
    20161 agcagaactc tccctggctt gctttgaagc atgggccata ggcgttaaag gcagggtggt
    20221 aaaaagtaaa gcacagagcg tgatcgaaat acggtttatc cgcgccactg agcgtccctc
    20281 tgttaaggca ggtctatcat gcctgcgtct gatttacaaa acttgatgat tctaatgcat
    20341 agaagaagag aaagttaata gcttttattg ttaaaaaata cgtttcgttg gcgagcgcaa
    20401 aaaaatgcag cagtgaaagc taaaaatggt tataaatacg ccagtttgcc gagcatctgg
    20461 gaagaaacga atatttaaaa tgcaacaaat agagcaaagg gataaaatag caaaagcgtg
    20521 atgaaaatgt tattttccga tgccgcactg acccgctaca atgtcagatt actgccgtaa
    20581 agaagtttga ggaagcaaga caatgagcac cactatcgaa aaaatccaac gccagattgc
    20641 tgaaaacccg atcctgctgt acatgaaagg ttcaccgaaa ctgccgagct gcggtttctc
    20701 tgcccaggca gtccaggcgc ttgccgcatg tggcgaacgt tttgcctatg ttgatattct
    20761 gcagaacccg gacattcgtg cggaactgcc taaatatgct aactggccga ccttcccgca
    20821 actgtgggtt gacggcgagc tggtaggcgg ttgtgatatc gtgatcgaaa tgtatcagcg
    20881 tggcgaactg cagcagctga tcaaagaaac tgccgctaaa tacaagtctg aagagccgga
    20941 cgcggaataa tccgtcctgc aatactgacg gatgcgaacg ggcatccgtc agtcctttct
    21001 ctatccccaa tccagccctt gtggcgaact tgaaaatccc gcctcacgca gcagcgtaaa
    21061 catcggcgtt tgccggaccg gtagatcatt cacttctgtt agcgtaaagc gcagccgtgg
    21121 ttcgcgacgc agtgcggtag tcagcgcgtg gaaaacctct ggggcgagta attcctcttt
    21181 ttcctgccag accagcattt ttttgccacc ttgcgccaga tagagtaaca atttgccgcc
    21241 agaaacgacc accagcgcac ccgcccgacg cgttggaacc agcgttgccg gatgtgcagg
    21301 ccagggcaga agatttcccc acacattagc cggatcgttg gcagagagcg ccactggtgt
    21361 atagtggcgc gtttgcgtgg cttgtgtcgc cagatcgcgc aatcggtcaa tagtcagacg
    21421 ttcagcgaat tgcgcgccac ccagaccttc tacaaaacga cctcgcataa ttcgcccgga
    21481 gtcttccata cttcgacaaa gcgtttgcat cgatggaaac ccgccaggga tattttcggc
    21541 tatcaccgcc tgacgactga tgatgccgta gcggtcgagc atattttccg ccagcgccag
    21601 catcctttcg gtatcgttta gtggctccac ctgcaataac gaccagcgtc cagccagatt
    21661 tggtgtgttg taagatacca gcggcgagac gggttgcgca tagacaggac gtccacgacg
    21721 agcccggtga ctgcggcgag ttgaggtgcg tgcgttggaa ctgctgcggg tgagggcgcg
    21781 taacggtgcc caaatgtcgc tggtgatgac gccttgccag accagcgccc ataacgcctc
    21841 ttgcagggca gagagatcaa ccgattcgcc gattttgtcg cgtatccgtt ggctgatttg
    21901 ttgtgcaaac caggctcctc cgtcagccag aacagcgatt atcgcttgct gcagcgccga
    21961 acgattcgcc tgatccgctt cggcgggagt gaacgattct gcggcatatt cctgtagatg
    22021 cagtgccacc aggccgtcat cttcacccag ctttttttgt cccgaccaga taaccgctcc
    22081 ggttgccagt aattcatcga gcatttctgg cgaatagtcg cgtacgcggg caggcaggat
    22141 ctggctttcc cagagtgagg cgggtaaacc gactccggca agctgttcga tcacccgcat
    22201 cacgccatct acgccctcat aaacgcctgg cgatgttgag gcaaagagcg ccgggctacc
    22261 atcggtggcg ggtaatacgc cctgacgttc cagcagcaat cgcgcatagg tcgtggctgc
    22321 aacgggacgc gtcgcttctc tggcggcttg cagcgagcgc aaacgcagac gacgaaatac
    22381 ttcatcgctc acccagatgt cttgttgcag attcatcacc agaccctgtt cacgcagttg
    22441 ctgaagctgc tcttcgacaa tggcaatacc gagactaaac tcatgagcca gttgttcagc
    22501 cgtgaccaga gcatgagccc ggagatagcg cagaaagagg tcgcgaagcg ggtaactgac
    22561 tctatgtaaa taaatctctg gcaatgactc tggtagtcgt acgccgaggg catcacgcag
    22621 cctggcggca tcatccatac aggcaagacg ctcctgtccg ctaatcatcg ctgggaagat
    22681 tcgttttact gcaagaagat tttccagata cgacgcaacc tcttcactgc tgcctgtatg
    22741 ccgttgcgcc aggtcttcaa cggtcattgg ccccagttcg cgcagcaggt cgaacaatcc
    22801 ttcttcacct ttcgctcttc tgccaggagc cagtcgttgc aactcttctt ccacctggcg
    22861 aatgacctgc gggtcgagta attcccccgg atcgacctgt ccgagtagat tgcgcagtaa
    22921 ctcgctgtcc agcgacagta cggatgcccg gcgctctgcc agcggggcgt cgctctggta
    22981 cataaattcc gcgacatagc cgaacaataa acttgtggcg aaaggcgagg gcgtagtggt
    23041 cgttacatcg gatatttgaa tttcgccacc gttcaggcga cgcatcaaac gttccagtgc
    23101 gggaagatca taaacatctt gcagacattc gcgtagggtt tcgagaatga ccggaaaatc
    23161 cggatatccc tgagcgattt ccagcaactg actggcgcgc agccgttgtt gccaaagcgg
    23221 ggtgcgatgg cccggagtgc gccccggcat taataatgcc cgcgcggtgc attcgcgaaa
    23281 acgggcggcg aaaagtgccg agctgcctac cgcctcgcgg acaatttgca gcaacttttc
    23341 tggttcaaac aaaaaaatcg cggcgtcggg caatttacca tcagtgtcag gaatgcgcgc
    23401 aacaatgccg tcatcactgg cgaccacaga cgcgtcagcg ccccataggg catgtattcg
    23461 cccggcaatc gccagcgccc agggttcatg cacccgtctt ccatagggag agtgcaaaat
    23521 aatacgccag tcgccaattt catcacggca ccgttcgagg accagatggc gactccccgg
    23581 aacgatgccc gtcgcgttgc gttgctcgtc aatcaacccc tgaatattgg cgttcgtatt
    23641 ttcttctgcc agccacgggg gaatagtccc ggaaaagaac gcgccatccg ccagtaaatg
    23701 aagaaaatcg ccgatcatct cgcctaattc agccggacgt ccgttacctt caccacgcca
    23761 gaaggggagc cgggcagaac gacccggagc aggagtcaca atcacctgat cgcgggtgat
    23821 ttgctggatc cgccatgagg tagcgccgag agtgataatg tcgctcaccc gcgactcata
    23881 taccatctcc tcatccagtt cacccacccg ccgcgaaccg gcctgttctt caccttcggg
    23941 taataacacg ctatacatgc cacgatccgg aatggtgccg ccgctggtaa ccgccaacaa
    24001 ttgagcgcca ggtcgggcgg tcaatatccc ggtctccctg ttccagacca gtttggggcg
    24061 aaaagcagaa aaatcgccag agggatagcg cccggaaagc atatccagcg tggcgtcaaa
    24121 aacacgtctt ggcagatctt tccacggtgc ggcacggcgt acgcgggagt accattcgtc
    24181 tacctgtaat gcatccatcg ccgcggcggc aacggtttgc tgcgcaagga cgtcgagtgg
    24241 attatgcggc ggtgtcaggt tttccagcct gcctgcgaac atacactcta caatgactgc
    24301 ggaatcgact aagtcacgcc gggtacgagg gaaaaacagc cctttagata caccgccaac
    24361 ctgatgtccg gcgcgaccaa tgcgttgtaa cccactggca acagaaagcg gcgttgccac
    24421 ctgaatcacc agatccaccg cgcccatatc aatccccagt tcgagactgg aggttgcgac
    24481 cacgcagcgt aattcacccg atttcagcgc ctgttcggtg attgctcgtt gttctttaga
    24541 gacggagccg tggtgtgagc gggcaataaa aacgtcgcta ctttgtacat ggttagaggt
    24601 tgcgccggag gtcgactcga aatgggccgt atcaacggcg atagacgggg aacgctgtaa
    24661 gcgtgcggcg taaagctcat ttaatcgtgc cgtcagtttt tccgccagcc cacgtgaatt
    24721 agtaaagaca atggtcgagc gatggcgcaa cacttcatca aggatacccg tttcaatata
    24781 tggccagatg gagccttccc ggccggcatg gctgtcttcg ccggtgccgc tggcgaccga
    24841 tgagacatca tccatattgg cgaccggtac gacaattcgt atctgcggat ggcgcattgc
    24901 gggcgggttg actaccgtaa ccgggcgatc gccaccaaga aatgctgcca catcgctggc
    24961 tgagcgcaca gtggcagaaa ggccaattcg ctgtgctgag gtgtggagca gcgcatcgag
    25021 ccgctccaga cttaacgcca gatgcgcacc acgtttactg cccgctaccg cgtggacttc
    25081 atcaataatt accgtttcga cgccgcgtag cgtttcgcgc gcgcgggagg tcagcatcag
    25141 atagagtgat tcgggtgtgg tgatcagaat atccggcgga ttacgggtga gtttgctgca
    25201 ttcctgtgca ggcgtatcgc cagtacggat ccctacgcga agattgactt ccgtttcgcc
    25261 gcgccgccgc cgttcatcgg caataccctt caacgggagc tgcaagttgc gctgaacgtc
    25321 ggtgcccaga gcttttatcg gtgaaatata gaggatgcgt gaggttttac gcttatgcgc
    25381 ctcgcgggta tcttcgccgc cttcgcggaa gagccgatcg agggcgtaga gaaatgctgc
    25441 cagcgttttc ccggagccgg tcggtgcaat caccagcgca tgttcgcttc gcgccgccac
    25501 atgccaggtt tgcgactgga cagcggtcgg ctgtttaaag gcgcgaagaa accagtcgcg
    25561 ggtcgccggt gaaaacacgt ccggcaggag cgatgaaggg tctggattat ctgccattaa
    25621 cgctattccc cgtgaatatc atgcttgatg cgtggaatag cctcactata gatgcctgaa
    25681 tcattgcatg tcatcaggca tcgactcgat tacacctctt cggcggcagg tagcggccag
    25741 cctcccagac gtttccagcg gttgacgatt tcacaaaaca gcacagcagt gcgttcggtg
    25801 tcgtacagcg cggagtgcgc ctgggtgctg tcgaagtcca tgccagcggt ctggcaagcc
    25861 tttgacaata cggtttgtcc gagtgccagc ccggccagtg cagcagtgtc aaaagtggcg
    25921 aaagggtgga acgggttacg tttcagtgag gcgcgttctg cggcggccat cataaagctg
    25981 tgatcaaaat tggcattgtg cgccaccata atggcgcggt tacagccgct cgctttaata
    26041 cctttacgta caactttaaa aatttcgtgc agcgcctcgt attcgctgac cgcgccgcga
    26101 tcgggatcgt tcgggtcaat gccgttgaag gcgagggcct ctggctgcaa atttgcgccg
    26161 acaaatggtt cgacgtggaa atgtaatgtg gtgtccggca tcagccagcc ttgttcatcc
    26221 attttcaggg tgatggcggc aatctcaagc agcgcatcgg ttttggcgtt aaatccggct
    26281 gtttcaacat cgatcacaac aggataaaaa ccacgaaaac ggtcgcacag accggtaagt
    26341 tgagcgttat cggacatctg attctcttaa tacggataaa ttgcagcgcg cattatgaca
    26401 aatattactt gtcgatgcag taaagatgcg ggcgcgatga gttcacgccc ggcaggagat
    26461 tagttgccca gaccgcgacc ggcgtctttc tcttcgatta actcaatttt gtaaccgtcc
    26521 ggatcttcca caaacgcgat aaccgtagta ccgcctttta ccggacccgc ttcacgggtc
    26581 acgttacccc cgttttgacg gattttttcg cacgcttcag cggcgttatc tacgctaagc
    26641 gcgatgtgac cataagcagt gccgagttcg tatttatcca cgccccagtt gtaggtcagt
    26701 tcaatcaccg cttcttcggt ttccgggccg tagccaacaa acgccagtga gtatttgtat
    26761 tccgggtttt cgctggtacg cagcagtttc atgcccagca ctttggtata aaaatcgatg
    26821 gagcgttgca gatcgccaac gcgcagcatg gtatgaagaa gacgcatctt tttatcctca
    26881 aaatggctaa cagattcaaa atgttgtttt agtatagcgg cggctttacg ccgctcgcaa
    26941 tgttggatta caacgtcggg taatcggtat agccttccgc gccgccaccg tagaaacttt
    27001 cggcacgctg tgggttaagc tcagctttgc gctgcaagcg ggcgaccaga tccgggttcg
    27061 caatccagtc acgaccaaat gccaccgcat caattaaccc tttgccgatc agcgtttcag
    27121 ctttttctac tgtgtatgca cctgcgccga taatcggacc gtggaaacgg gcgcgtactt
    27181 tttcgcggaa cgcatcagta tacggttcac cccccgccca atctggttct gacatatgca
    27241 gataagcaat gccgcgttta cccagttgtt caatcagata cagtgcatcg gcttcttcat
    27301 tcgcgccgtt atccgtgttc tggaaagtac cgattggcga aacgcgaatg ccaatgcgat
    27361 cggcacccca ttcttcaatc ccggcatcga ccacttccag taccagacgt gcgcgatttt
    27421 ccacgctgcc gccgtactga tcggtacgat ggtttgaaga aggagaaagg aactgatgca
    27481 gcaaataacc gtgagcagag tggagctcta ccagatcaaa accggcttca cgcgcgttag
    27541 caatggcctg acggaaatca ttgacgatac ctggaatctc ttccagttca agcgcacgcg
    27601 gcatggatgt ttcaacacgg atcgcctgac cattttcatc gcgcagagaa gtacgtgttc
    27661 ccgcgctaag tgctgaaggc gctaccggtg cctgaccgcc aggttgcagg ctggcgtgag
    27721 aaatgcgtcc ggtgtgccac agctgcacgg ccatatgacc attttcagca tgaacgccag
    27781 cggtgatttt tttccatgcg gcaatttgct ccggactatg gatgccaggc gcacctgcat
    27841 atccttttgc ctgggcagaa atttgcgtgg cttcactaat aatcaaaccg gcactggcac
    27901 gttggcgata gtattccgcc atcaacgggg taggaatgtc acccggttca atactgcgca
    27961 gacgcgtcag cggtgccata aaaatacggt ttgccgccgt gatcgcgccc actttcagtg
    28021 gggaatacag tttttcagat gacataaagg ctcccggtcg tctggtgata aaaaatgcct
    28081 gctaaacggc aggcgtcgca ataatgtttt ttacatgggc cagcgcgttt tccagtggct
    28141 cgaaactgcg cgaaattttg gcctgcagat tcgcacccag ccatagtgcg taaagcactt
    28201 gtgcctgttg tagcggttcg ccacaaaagg ttaaacaatg gttctcacgg ccattttcca
    28261 gcgcctgaga gagcagggcg atcacgccgc gagcaccttt atccatcgcg ctgcgcatat
    28321 cttctgacag atcgcacact tcggcagaga gttttactgt caggcaacca ctgatggttc
    28381 catgttggca aaactggttc agtgtttgct ggtaataagc cagtatgcgg tcgcggtagt
    28441 taccttcgcc ggattgcagc aactcagtca gtcgctggtg atatgcggcg taatgacgct
    28501 caagcatggc aacgccaaac gcttctttag agcgaaagta gtgatagaag gaccctttcg
    28561 gcacttcagc ggtttttagt aattcgctta gccccatccc ggtgaatcca cgttgcaggc
    28621 aaagttgctc gcccgtcgcc aggagatgtt cgcgagtatc atgttcggtg tgtttgttca
    28681 tgcgttggag tgtagtagac cagtcggtct agtgcaagca aagttgctac tttggacatt
    28741 aacagcttca ataatggtaa taatcttatc tgaggagggc gaagtggcgg agcaattaga
    28801 gttctttccc gtccagagcc cgtgccgggg aatttgccag tctgacgaac gcgggttttg
    28861 tcgcggctgt tttcgtagtc gtgatgagcg ttttaactgg aataaaatga gcgatggcga
    28921 aaaacaagag gtgctgcgcc tttgtcggca gcgattaatg cgcaaattac gcgcaaataa
    28981 gccagcgtca tcagacgaac cggagcaacc atcactcttt tgaacctaat cttgcgtata
    29041 ctcctctcat atctgataag aggaagtgat tatggttcag cgtattacta ttgcgccgca
    29101 aggcccggag ttttcccgtt ttgtgatggg ctactggcga ttgatggact ggaatatgtc
    29161 cgcccgccag ctggtcagtt ttattgaaga gcatctggat ctcggcgtga ccaccgtgga
    29221 ccatgctgat atttatggtg gctatcagtg cgaagcggcg tttggcgagg cactgaaact
    29281 ggcacctcac ctgcgtgaac ggatggaaat cgtcagtaaa tgcggtatcg cgacgaccgc
    29341 gcgtgaagaa aacgtcattg gtcattacat cactgaccgc gatcacatca ttaagagcgc
    29401 cgaacagtcg ctaattaatc tcgcgaccga tcatctggat ctgctgttaa tccaccgacc
    29461 agacccgtta atggatgccg atgaagtggc ggacgcgttc aaacatctgc atcagagcgg
    29521 caaagtgcgt cattttggcg tatcgaactt tacgcctgcg caatttgccc tgttgcaatc
    29581 acgtctgccg tttacccttg ccactaatca ggtggaaata tccccggtgc atcagccgtt
    29641 actgctggat ggcacgctcg accaactaca acaactgcgt gttcgtccga tggcgtggtc
    29701 ctgccttggt ggtggtcgtc tgtttaatga tgattatttc cagcagctgc gtgatgaact
    29761 ggctgtggtg gcagaggagt taaacgcggg ctcgattgaa caggtggttt acgcctgggt
    29821 attacgttta ccatcgcagc cgctgccaat tatcggttca ggtaaaattg agcgcgtacg
    29881 ggcagctgtc gaagcagaaa cactgaaaat gacccgtcaa caatggtttc gtatccgtaa
    29941 agcggcactg gggtacgacg taccgtaagc acttttagga atagccgccg ttcaaaaatg
    30001 tgtcactggt ttacacttat tcaggaatgc acaatgaacg gaggtcctat gaaacgtttt
    30061 agtctggcta ttctggcgct ggttgttgca accggcgcac aagctgccag tgaaaaagtc
    30121 gagatgaacc tcgtcacgtc gcaaggggta gggcagtcaa ttggtagcgt caccattact
    30181 gaaaccgata aaggtctgga gttttcgccc gatctgaaag cattaccccc cggtgaacat
    30241 ggcttccata ttcatgccaa aggaagctgc cagccagcca ccaaagatgg caaagccagc
    30301 gccgcggaat ccgcaggcgg gcatcttgat ccacaaaata ccggtaaaca tgaagggcca
    30361 gaaggtgccg ggcatttagg cgatctgcct gcactggtcg tcaataatga cggcaaagct
    30421 accgatgccg tcatcgcgcc tcgtctgaaa tcactggatg aaatcaaaga caaagcgctg
    30481 atggtccacg ttggcggcga taatatgtcc gatcaaccta aaccgctggg cggtggcggt
    30541 gaacgctatg cctgtggtgt aattaagtaa ttaagaggcc agcgtacctt gcggtggtgc
    30601 ttgctcaagt tgcgaaagcg agcagtacag ccgccagaca attgccgcca gctcacgggc
    30661 ggcaggttga tgatgacggg caaggctgtc gcaaatccgc tgtaattctt caagtgtggc
    30721 cgccagtgat ttttgctgaa cgccacgctc actcatcact ccccgcaaca gtgaaataca
    30781 gttatcccgt actcgcgata acggatcgga acgcgattcc cagtcgcgca attgccagac
    30841 aacatgagaa cagttcagca gcactacacc ccagcgtaat aaccaacggc gagccagcgc
    30901 atcctggctg ttactcaact gactgacgtg atgataagtg agcgattcaa attcgctttc
    30961 actcagtgtt ggatggcggc ttagctgatc gacaaaatcc cggcgcagcg cgcgaatatg
    31021 gcggcggctt ttacgagcat ccgatcctgg acgcagaatg gcgaacgcta gccaagccag
    31081 cgcaacgcca acgatttttg ccagattatc gttaagaaaa tcagcaaaat catacaccgg
    31141 gggattagtg acagcgataa acgaacccat aaaaacaatc agttgccccc acagtgcggc
    31201 aaatttgggc atctgcaatt taagaagctg cattgtcgcc agcagtggaa agagaaacag
    31261 taaaaattgc cacagatcgc taatctggac catcaggcca aatttgacca caaagctgaa
    31321 tagcgaaagt aacaccagcg tgcgcatcag cagtgacaac gacttaaacg gtgctgcgac
    31381 ggcggagtag agtacgcagc taattgctgc cagcgttaat gcattggcac cggcatccca
    31441 ttgcgatgca atactccatg cgccaatcat catcaacgta caaaatgtac gcagcccgct
    31501 ccacatagct tcggcgttgt cggtatgacg agccagagcg gatgtccgtg cgagtctggc
    31561 atgatcatct acaccgcttt gcagacgatg taattcctga ctactttgca gataaaggcg
    31621 gcaaaaataa cgtagtcgct gccagaaggc gacgtgccgg tagtcggcga cgttggtcgg
    31681 gcgtagcggg gcgataatac gtgcgacggt gtaagcatct gtttacgaac tggcgagcgc
    31741 cgtcagcaat tgttcgagaa tttctcgcgt ggcacctggc ggtgagggcc agttgagcaa
    31801 catacgtcgc aggctggaga tgacactggt catacgtaat tgctggtgga gtagcgcatt
    31861 aaggcgcgcg ttttgctggc gaaaacgata gtggctccag aaagcctgga tacgcagcaa
    31921 attcatggtc agtatctgcc caatcacccc ctcatgtgct gcacgaatgg catcgtttgt
    31981 ttcaggctgc cagagtaaac tggcatgttc cagtaatcgg gcgtgcatgt ttttcaatgc
    32041 ggttaaaagg gctgtagcat cggaactgct aggcaggatc atcatcatca tgccgccgca
    32101 caaaataccg actatcacct cgcaaacgcg cgcctgagcg atatcccaca gctggctggc
    32161 ctcagtaata ttaaccatcg gaaaggcgat gatggcagcc gtgtagcccg ccagttgaaa
    32221 tgcatacgcg acgttattcg tgaagtgcgc acaggcccag gtacaaaagc caagccacgc
    32281 cgacatgctc aatagaaaaa accacggctc attgagcgta tgcccggcaa gaagcagtgc
    32341 cgcaatggct cccagtaaac tgccagcgat gcgtccgagg cttttgctga tgacaccgcc
    32401 aacggtggga aagctaacca ctgcagccga ggtcatcgcc caatagggtt catccagatt
    32461 caaataatag gcaaccgtca gcgccagaca catggcaatg gtattgcgta acgcataacg
    32521 ccattgcgcc agcgtggcct tgaaccaggg caaattgcgc aaggaccatg acgatgcgtt
    32581 cattattgac ctacggcaat gctacaggtg gtgcccgaca ccagcgtgac atcgcctgga
    32641 actttatcca gcgcaaagcg gacgggaacg cgttgggcga gacgaaccca ggggacgtta
    32701 ggtttcacat ccggaataag gcttgagtca ctttcaacgc tttgatcata aatcgcccga
    32761 ccgatactgg aaacgtgacc ccgtaacgtt ttgttatcac tatagagggt aatttgtgct
    32821 ggtgcacctt cgcggatatg gcgcaacttg gtttcttcaa aataaccaat gacgtaaaac
    32881 gagtggctgt cgacaagggc aaacagcggt ttcccggtgt cagcgtaatc gccgatgcgg
    32941 gtagtgaggt tagtcaccca accactcacc ggggcgcgaa tttccgtttg cgccagttgc
    33001 cattgtgtct gtttcagcgt ggcctgtgcg gcattaacac tggcctgcat cgctttaaca
    33061 ttgaggttag cggtatcgag ttcttcggca gaaataaaat tttgtgagag atgacgacga
    33121 cgattcgctt cgttattggc ttttgcgagg tcagattgcg ctttcgccag ctgagcctgt
    33181 gcgttcagct cggcgatctg aaagggcgtt ttatcgatcg tgagcagaag atcccccgcg
    33241 ttaaccagtt ggttgtcttt tatattcagc tcaacaatac gtcccgacac ctgtggggtg
    33301 atagaaacct tttctgcgcg tattttcccg tcgcgtgtcc acggagattg catgtaataa
    33361 ttccacagcc accatccggc aagaaccgca actatcgcta caatgattgt tgagaaatac
    33421 ttaattgttt taattgacat agttaccacg caatcagtat tgccagagca aggcaaacgc
    33481 aaatcgcaaa cagcgataaa tccattaaca agggatgcca gatgtcaccg gcgtagatcc
    33541 agccacgaag caggcggtgt acgacaagcc agatgacgaa tccaaacgcg aaggctttga
    33601 aaaacggagg aaaatagacg gacgcaccga acaccagatc ttgtaagggc aatcctgttg
    33661 cattgagcat aaacttcaca aagtagattc ctttacgacc gaatatattg cgttagatta
    33721 ataaatattc tttaagtgcg aaaaatttac gcgcaatttc tgaaaaatat gtctgacggt
    33781 aaccaaatgc agcaatacat ttgttttagc aatacaattg ctgcacacta ttctaaaagc
    33841 cgcataatat cttagcaagc taattataag gagatgaaat tggaatcgcc actaggttct
    33901 gatctggcac ggttggtgcg catatggcgt gctctgatag accatcgcct gaaaccgctg
    33961 gagttaacac aaacccattg ggttacgtta cacaatatcc atcagttacc tccagaccag
    34021 tcgcaaattc aactggcaaa agcgattggc atcgagcagc catcactggt ccgtactctg
    34081 gaccaactgg aagaaaaagg gttaatttcg cgtcaaactt gtgccagcga tcgtcgggct
    34141 aaacgtatta aactgacgga aaaggcagag ccactgatca gcgaaatgga agctgttatt
    34201 aacaaaaccc gcgcggaaat attacatggc atctccgcag aggaactgga gcaactgatt
    34261 acgctcatcg caaaacttga gcataatatc attgagttac aggccaaagg gtgaaatgaa
    34321 gggggcgtgt ggtcagtaac ctgaccacac gcaaacttat ttagcgcgga gaaacggtca
    34381 cctgactgcc attgctggcc agtacgacac gttggcccgg agagaaacga gtgttgcctt
    34441 gtttctgtac caccatgatg gtattaccat cgtctttacg aatttccagc tcgacaccct
    34501 gcgttttgtt cattgcactc tgtacgccct gaccagctac gccacctgca acagcgcctg
    34561 ctgcagtagc cagagaacgc ccggttccgc caccaacagt attccccagg aaaccaccaa
    34621 gaacagcacc gccaattgca ccgataacgt tggaatcatc accgccctga atctgtaccg
    34681 gacgtacgtt aacgatggtg ccatagctga cattctgtac ttgtttcgct tcagaagcgg
    34741 tataaacatc ccctgacagg gtgtcgttat taacacaacc gacaagagac agacctacca
    34801 ttgaaacaac caatacgcgt ttaatcattg aaaaatctcc tgttcaccat gaaacgccac
    34861 gcaagcatcc ctcatggtca aagtatatgg catatgtgaa tccatggtta catgttctac
    34921 caaatatgag caaatcataa tgaaagtagg atcaaccaat tataaacaaa acattcatga
    34981 tgattattga tttgaagata atgttttact accaacggta aaaatcggtc attccgcatg
    35041 gtattcacga aaagtttatg tttgagtggt gcgcaaacgt cagattgaaa aaggatgaac
    35101 tatgaaatcg ggccgcttta ttggcgttat gtcaggcacc agccttgatg gtgttgatgt
    35161 tgtgttggcg acaatagatg aacaccgggt cgcacagctg gcaagcttga gctggccgat
    35221 cccggtgtct ctgaaacagg ctgtactgga tatttgccag ggccagcagc ttacactttc
    35281 gcagtttgga cagcttgata ctcaactcgg gcgacttttt gctgatgcgg tcaatgcctt
    35341 gcttaaggaa caaaacctgc aggcgagaga tatagttgcg atcggctgtc acggtcaaac
    35401 cgtctggcat gaaccgacgg gcgtggaacc acacacttta cagattggcg ataacaatca
    35461 aattgtggca cgcaccggaa ttacggttgt cggtgatttt cgccgtcgcg atattgcctt
    35521 gggaggacaa ggcgcaccgc tggtacctgc gttccatcat gccttgctgg ctcacccaac
    35581 cgagcgacga atggtgctca atattggcgg catcgccaat ctgtcactgc tcattcctgg
    35641 gcagccggtt ggcggctacg ataccggtcc tggtaacatg ctgatggatg cctggatctg
    35701 gcgtcaggcc ggtaaacctt acgataaaga tgccgagtgg gcacgggcgg gtaaagttat
    35761 tctcccactg ctgcaaaata tgctcagcga cccatatttc tcgcaacctg caccgaaaag
    35821 caccggacgc gaatacttta actacggctg gctggagcgc catttacgcc attttccggg
    35881 tgttgatccc cgtgatgttc aggccacact ggcagaactc accgccgtga ccatttctga
    35941 acaagttttg ttgagcggtg gctgcgaacg attgatggta tgtggtggag gtagtcgtaa
    36001 tccgctactc atggcgcgtc tggcggcatt actgccaggc acagaagtca ccaccaccga
    36061 tgccgttggc attagtggcg atgacatgga agcattggct ttcgcctggc ttgcctggcg
    36121 gacgctggcg ggattaccag gaaatctgcc ttccgtcact ggcgcaagcc aggagacggt
    36181 actgggggct attttccctg ctaacccgtg atatcagagt taactgaatt tttcttaccg
    36241 cgtctgccgt tacacttgga acgttaggga gggcgtattg ccctccagac caggaaagtc
    36301 ttcgggaaat gtctatgaaa aaactgttac tgatctgttt gccggtgctt ctcaccggct
    36361 gtagcgcgtt caatcaactg gttgagcaca tgcaaaccgc tacgctggaa taccagtgtg
    36421 atgaaaaacc gttgacggtc aaactgaata atccgcgcca ggaagtcagt tttgtttacg
    36481 ataatcaact actgcatctc aaacagggca tttcagcgtc tggtgcacgt tacactgacg
    36541 gaatctatgt tttctggtcg aaaggcgatg aagcgactgt ctataaacgc gaccgcatcg
    36601 tattgaataa ctgtcagtta caaaatccac agcgttgaga tttttccagg ggcggcgcac
    36661 aatagcgtca cccactgaca atccgtaaag aaaaccatgt ctgataacga cgaattgcag
    36721 caaatcgcgc atctgcgccg tgaatacacc aaaggcgggt tacgccgtcg cgatcttccc
    36781 gccgatccat taaccctttt tgaacgttgg ctctctcagg cgtgtgaagc caaactggca
    36841 gaccctaccg cgatggtggt cgctaccgtg gatgaacatg ctcagcctta tcaacgcatc
    36901 gttttactca aacattacga cgaaaaaggc atggtgtttt acaccaacct cggtagccgt
    36961 aaagcacatc aaattgaaaa taatccgcgc gttagcctgc tgttcccgtg gcataccctt
    37021 gaacgccagg tgatggtgat cggtaaagca gaacgacttt cgactctcga agtgatgaaa
    37081 tattttcata gccgcccgcg tgatagccag attggtgcat gggtttcgaa gcagtccagt
    37141 cgcatttctg cccgcggtat ccttgaaagt aaattcctgg agctgaagca gaagtttcaa
    37201 cagggcgaag tgccattgcc cagcttttgg ggcggttttc gcgtcagcct tgaacagatt
    37261 gagttctggc agggcggtga gcatcgcctg catgaccgct ttttgtacca gcgagaaaat
    37321 gatgcgtgga agattgatcg tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg
    37381 ctggtactcc tgattctggc actttattct atgtctcttt cgcatctggc gaaaagtcgt
    37441 gtaccggcaa aggtgcagtc gttatataca tggagatttt gatggcaagc agtaacttga
    37501 ttaaacaatt gcaagagcgg gggctggtag cccaggtgac ggacgaggaa gcgttagcag
    37561 agcgactggc gcaaggcccg atcgcgctct attgcggctt cgatcctacc gctgacagct
    37621 tgcatttggg gcatcttgtt ccattgttat gcctgaaacg cttccagcag gcgggccaca
    37681 agccggttgc gctggtaggc ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg
    37741 ccgagcgtaa gctgaacacc gaagaaactg ttcaggagtg ggtggacaaa atccgtaagc
    37801 aggttgcccc gttcctcgat ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact
    37861 atgactggtt cggcaatatg aatgtgctga ccttcctgcg cgatattggc aaacacttct
    37921 ccgttaacca gatgatcaac aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg
    37981 ggatttcgtt cactgagttt tcctacaacc tgttgcaggg ttatgacttc gcctgtctga
    38041 acaaacagta cggtgtggtg ctgcaaattg gtggttctga ccagtggggt aacatcactt
    38101 ctggtatcga cctgacccgt cgtctgcatc agaatcaggt gtttggcctg accgttccgc
    38161 tgatcactaa agcagatggc accaaatttg gtaaaactga aggcggcgca gtctggttgg
    38221 atccgaagaa aaccagcccg tacaaattct accagttctg gatcaacact gcggatgccg
    38281 acgtttaccg cttcctgaag ttcttcacct ttatgagcat tgaagagatc aacgccctgg
    38341 aagaagaaga taaaaacagc ggtaaagcac cgcgcgccca gtatgtactg gcggagcagg
    38401 tgactcgtct ggttcacggt gaagaaggtt tacaggcggc aaaacgtatt accgaatgcc
    38461 tgttcagcgg ttctttgagt gcgctgagtg aagcggactt cgaacagctg gcgcaggacg
    38521 gcgtaccgat ggttgagatg gaaaagggcg cagacctgat gcaggcgctg gtcgattctg
    38581 aactgcaacc ttcccgtggt caggcgcgta aaactatcgc ctccaatgcc atcaccatta
    38641 acggtgaaaa acagtccgat cctgaatact tctttaaaga agaagatcgt ctgtttggtc
    38701 gttttacctt actgcgtcgc ggcaaaaaga attactgtct gatttgctgg aaataatgca
    38761 ttaagtggaa agggggagtg agaaatcact ccccctggtt tttatacagg gaacatgatg
    38821 aaaaatattc tcgctatcca gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca
    38881 gagtttccta tgcgccgcct gggcgcgaac gtctggccgc tgaacaccgt tcaattttct
    38941 aatcacaccc aatacggcaa atggactggc tgcgtgatgc cgcccagcca tttaaccgaa
    39001 attgtgcaag gcattgccgc cattgataaa ttacacacct gtgatgccgt gttaagtggc
    39061 tatctgggat cggcggagca gggtgaacat atcctcggta tcgtccgtca ggtgaaagcc
    39121 gcgaatccgc aggcgaaata tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt
    39181 atcgttgcac cgggtgtcgc agagtttcat gtgcggcacg gtttgcctgc cagcgatatc
    39241 attgcgccga atctggttga gctggaaata ctctgtgagc atgcggtaaa taacgtcgaa
    39301 gaagcggttc tggcagcgcg cgaactcatt gcgcaagggc cacaaattgt gttggttaaa
    39361 cacctggcgc gagctggcta cagccgtgac cgttttgaaa tgctgctggt caccgccgat
    39421 gaagcctggc atatcagccg tccgctggtg gattttggta tgcgccagcc ggtaggtgtt
    39481 ggtgatgtga cgagcggttt actgctggtg aaactgcttc agggggcaac gctgcaggag
    39541 gcgctggaac atgtgaccgc tgcagtctac gaaatcatgg tgaccaccaa agcaatgcag
    39601 gaatatgagc tgcaagtggt ggctgctcag gatcgtattg ccaaaccaga acattacttc
    39661 agcgcaacaa agctctgaaa tatctcattt cggcccacaa cgacctgtgg gccgatggat
    39721 attactttaa gccttccgct gacagcgcgt cttgtacttc cggacgttca gccatacgtt
    39781 gcataaatgc tgcaatgtgc tctaaccctt ccagattcag tttcaccgcg tatgcccagc
    39841 gcagaaccgt aaacagatag gcatcagcaa ttgtaaatct ttgcccgcag atccagtgct
    39901 catccttcag tgcctcgttc acatattgca gcttcttctc cagctgcgcg cgaactgtcg
    39961 gtttgtactc ttccggtgta tcagggcgaa acagaggtgt gaaaccttta tgcagctcgg
    40021 tggcgatgta attcagccat tcgatggttt tatagcggga aatactgttt accggtgcca
    40081 gcaactggcg gtcggggacg ctgtcggcaa gatactgcat aatcgctacg ccttccgtca
    40141 gcaaagtacc gtcatccagc agcaatgcag gcacctgccc cttagggtta acggcaaagt
    40201 aatcgtcacc gttttcgaga cgttttttca ttaaatccac actgacgagg gtaaaatcct
    40261 ttccgctctc acgcagggtg atatgggaag cgagagagca ggcacccggt ttgtagaaca
    40321 atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa
    40381 aaaaaagccg ctaacgaaaa gttagcggct tgtaaaagag tttccctgaa ttttacgcta
    40441 cagcagcttt cgccgctttg tctgcagcgt catcctgcgt catacggtgc agtttaggcg
    40501 cggtcagcag catcagtact gcaataatgg cagtagcgac accaatctgc aagaatacgc
    40561 gaccataaac ttccagtgac atcagcgggt cggtaacgtt atccggcaca gccatcatac
    40621 ccgcaacata accaccaatc aggtttgcac cggcagtggt caggaaccag ctacccataa
    40681 tgaagcccat cagacgctgc ggaacgagtt gtgcaaccat tgccagaccc agaccagaga
    40741 tcatcagttc cccgatgctc tgcaggccat agcttgcgac cagccagctt acagacacga
    40801 taccagcgtc agacgcgaat ttcgcaccca gcggcagaat caggaacgca ccagaacaca
    40861 tcaccatgcc gattgcaaac ttggttggca tcggcagggt atcgcccatc ttgttataga
    40921 tagcggccag aatcggacta ccgatgatga tccagaacgg gttcagtgcc tgatactgct
    40981 caggttctac ggccagaccc agaatggagt gctcaacgtt acgaatcgca aagaagttca
    41041 gtgatgtcgg catctggctg tacagcacga agaagatgat tgcttcgagc atcaggatga
    41101 aggcaacgat cattttacga cgcgcagcac ctttcatcgc gaaggcttct ttaccgaaga
    41161 taaccacgat accgaaggca acaacgccca gcgccatacg cgcaacttcc tgattgtgca
    41221 gcagccaggt ggcgatagcg atcagtgcca caacaccaat aatggttagc agcaggttac
    41281 ggtagttgat aggctcgaag tctggttttg aaccgtactg tttaacccag cgttggcaga
    41341 aggcgaagtt aacgatagtg atcagcaggc ctacaacgct caatgcaaac gcaacgctcc
    41401 agccgtattt cgcggccagc cacggcgtag caatcataga gaagaaagag ccgatgttga
    41461 cggacatgta gtacatggtg aatgcaccgt ccagacgcgg gtcgtttttc tcatagcatg
    41521 tagaaagcag agaagacggg ttagccttaa acaggccgtt accgaccgca atagccgcca
    41581 tacccatata aacaataccg gcgtcgtgac cagaccaggc aaccagagca taaccaatcg
    41641 ccagcacaat agcgccgagc ataattacgc gtttagtacc cagtaccttg tcacctaacc
    41701 agccgccgat agcgaccaga ccataaacca gggcactaaa ggaagagaaa agggtgattg
    41761 aatccgcttc agacataccc agttgtttaa ccaggtaaac agccataatt ccttgtaggc
    41821 cgtaataacc aaaacgttcc cataactcaa tcgagaagat gagatagaac gccttcggtt
    41881 gtttgaaagc gttcaaactg acgctttcag ttggtttttg gtttgcagtg gacactttta
    41941 cctctttttt tacatcccat attaacgggg gtgctttctc gcgttatgtc taaaaaacat
    42001 acgcgcaatt gttatagtgg gaggggaaac ggcaggtaat gttcactatc ttgcactttc
    42061 agacaagtcc tttgtaataa tctgtt
//
LOCUS       DAZGJW010000023        41956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZGJW010000023 DAZGJW010000000
VERSION     DAZGJW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.023"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..670)
                     /locus_tag="ACHKIK_001312"
     CDS             complement(<1..670)
                     /locus_tag="ACHKIK_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO9082520.1"
                     /translation="MSGKPAARQGDMTQYGSSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFH"
     gene            complement(830..1042)
                     /gene="rpmE"
                     /locus_tag="ACHKIK_001313"
     CDS             complement(830..1042)
                     /gene="rpmE"
                     /locus_tag="ACHKIK_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO9082521.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            1245..3443
                     /gene="priA"
                     /locus_tag="ACHKIK_001314"
     CDS             1245..3443
                     /gene="priA"
                     /locus_tag="ACHKIK_001314"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO9082522.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3599..4624
                     /gene="cytR"
                     /locus_tag="ACHKIK_001315"
     CDS             3599..4624
                     /gene="cytR"
                     /locus_tag="ACHKIK_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO9082523.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4716..5675
                     /gene="ftsN"
                     /locus_tag="ACHKIK_001316"
     CDS             4716..5675
                     /gene="ftsN"
                     /locus_tag="ACHKIK_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO9082524.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5768..6298
                     /gene="hslV"
                     /locus_tag="ACHKIK_001317"
     CDS             5768..6298
                     /gene="hslV"
                     /locus_tag="ACHKIK_001317"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO9082525.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            6308..7639
                     /gene="hslU"
                     /locus_tag="ACHKIK_001318"
     CDS             6308..7639
                     /gene="hslU"
                     /locus_tag="ACHKIK_001318"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO9082526.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7706..8632
                     /gene="menA"
                     /locus_tag="ACHKIK_001319"
     CDS             7706..8632
                     /gene="menA"
                     /locus_tag="ACHKIK_001319"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO9082527.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8725..9210
                     /gene="rraA"
                     /locus_tag="ACHKIK_001320"
     CDS             8725..9210
                     /gene="rraA"
                     /locus_tag="ACHKIK_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO9082528.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(9295..9540)
                     /gene="zapB"
                     /locus_tag="ACHKIK_001321"
     CDS             complement(9295..9540)
                     /gene="zapB"
                     /locus_tag="ACHKIK_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO9082529.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9965..10810
                     /gene="glpF"
                     /locus_tag="ACHKIK_001322"
     CDS             9965..10810
                     /gene="glpF"
                     /locus_tag="ACHKIK_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO9082530.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10833..12341
                     /gene="glpK"
                     /locus_tag="ACHKIK_001323"
     CDS             10833..12341
                     /gene="glpK"
                     /locus_tag="ACHKIK_001323"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO9082531.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            12476..13486
                     /gene="glpX"
                     /locus_tag="ACHKIK_001324"
     CDS             12476..13486
                     /gene="glpX"
                     /locus_tag="ACHKIK_001324"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO9082532.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13583..14329
                     /gene="fpr"
                     /locus_tag="ACHKIK_001325"
     CDS             13583..14329
                     /gene="fpr"
                     /locus_tag="ACHKIK_001325"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO9082533.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(14334..14762)
                     /gene="uspD"
                     /locus_tag="ACHKIK_001326"
     CDS             complement(14334..14762)
                     /gene="uspD"
                     /locus_tag="ACHKIK_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO9082534.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14789..15088)
                     /gene="yiiS"
                     /locus_tag="ACHKIK_001327"
     CDS             complement(14789..15088)
                     /gene="yiiS"
                     /locus_tag="ACHKIK_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO9082535.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(15300..15740)
                     /gene="yiiR"
                     /locus_tag="ACHKIK_001328"
     CDS             complement(15300..15740)
                     /gene="yiiR"
                     /locus_tag="ACHKIK_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO9082536.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15841..16440
                     /gene="yiiQ"
                     /locus_tag="ACHKIK_001329"
     CDS             15841..16440
                     /gene="yiiQ"
                     /locus_tag="ACHKIK_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO9082537.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16548..17315
                     /gene="tpiA"
                     /locus_tag="ACHKIK_001330"
     CDS             16548..17315
                     /gene="tpiA"
                     /locus_tag="ACHKIK_001330"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO9082538.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(17370..18125)
                     /gene="cdh"
                     /locus_tag="ACHKIK_001331"
     CDS             complement(17370..18125)
                     /gene="cdh"
                     /locus_tag="ACHKIK_001331"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO9082539.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(18232..19221)
                     /gene="sbp"
                     /locus_tag="ACHKIK_001332"
     CDS             complement(18232..19221)
                     /gene="sbp"
                     /locus_tag="ACHKIK_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO9082540.1"
                     /translation="MNKWGVGLTFLLAATNVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLV
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19540..20502)
                     /gene="pfkA"
                     /locus_tag="ACHKIK_001333"
     CDS             complement(19540..20502)
                     /gene="pfkA"
                     /locus_tag="ACHKIK_001333"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO9082541.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20683..21585)
                     /gene="fieF"
                     /locus_tag="ACHKIK_001334"
     CDS             complement(20683..21585)
                     /gene="fieF"
                     /locus_tag="ACHKIK_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO9082542.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21734..22234)
                     /gene="cpxP"
                     /locus_tag="ACHKIK_001335"
     CDS             complement(21734..22234)
                     /gene="cpxP"
                     /locus_tag="ACHKIK_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO9082543.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            22384..23082
                     /gene="cpxR"
                     /locus_tag="ACHKIK_001336"
     CDS             22384..23082
                     /gene="cpxR"
                     /locus_tag="ACHKIK_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO9082544.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            23079..24452
                     /gene="cpxA"
                     /locus_tag="ACHKIK_001337"
     CDS             23079..24452
                     /gene="cpxA"
                     /locus_tag="ACHKIK_001337"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO9082545.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24630..25304)
                     /gene="yiiM"
                     /locus_tag="ACHKIK_001338"
     CDS             complement(24630..25304)
                     /gene="yiiM"
                     /locus_tag="ACHKIK_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO9082546.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(25453..26436)
                     /gene="kdgT"
                     /locus_tag="ACHKIK_001339"
     CDS             complement(25453..26436)
                     /gene="kdgT"
                     /locus_tag="ACHKIK_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO9082547.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26696..27316)
                     /gene="sodA"
                     /locus_tag="ACHKIK_001340"
     CDS             complement(26696..27316)
                     /gene="sodA"
                     /locus_tag="ACHKIK_001340"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO9082548.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27601..28635
                     /gene="rhaT"
                     /locus_tag="ACHKIK_001341"
     CDS             27601..28635
                     /gene="rhaT"
                     /locus_tag="ACHKIK_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO9082549.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWSNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28632..29480)
                     /gene="rhaR"
                     /locus_tag="ACHKIK_001342"
     CDS             complement(28632..29480)
                     /gene="rhaR"
                     /locus_tag="ACHKIK_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO9082550.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQHVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLAMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(29554..30391)
                     /gene="rhaS"
                     /locus_tag="ACHKIK_001343"
                     /pseudo
     CDS             complement(29554..30391)
                     /gene="rhaS"
                     /locus_tag="ACHKIK_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
     gene            30679..32148
                     /gene="rhaB"
                     /locus_tag="ACHKIK_001344"
     CDS             30679..32148
                     /gene="rhaB"
                     /locus_tag="ACHKIK_001344"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO9082551.1"
                     /translation="MTFRNCVAVNLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDNF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            32145..33404
                     /gene="rhaA"
                     /locus_tag="ACHKIK_001345"
     CDS             32145..33404
                     /gene="rhaA"
                     /locus_tag="ACHKIK_001345"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO9082552.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARQALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            33487..34311
                     /gene="rhaD"
                     /locus_tag="ACHKIK_001346"
     CDS             33487..34311
                     /gene="rhaD"
                     /locus_tag="ACHKIK_001346"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO9082553.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAEVLVKIYSMGGMKQTITREELIALGERFGVTPLASALAL"
     gene            34383..35531
                     /gene="fucO"
                     /locus_tag="ACHKIK_001347"
     CDS             34383..35531
                     /gene="fucO"
                     /locus_tag="ACHKIK_001347"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO9082554.1"
                     /translation="MSFMLALPKISLHGAGAIGDMVNLVAHKQWGKALIVTDGQLVKL
                     GLLDSLFIALDQYEMSYHLFDEVFPNPTEELVQKGYAAFQDAACDYIIAFGGGSPIDT
                     AKAVKILTANPAPSTAYSGVGKVTNPGVPLVAINTTAGTAAEMTSNAVIIDTARQVKE
                     VIIDPNIIPDIAVDDASVMLEIPASVTAATGMDALTHAVEAYVSVGAHPLTDANALEA
                     IRLITLWLPKAVDDGHNLEAREQMAFAQYLAGMAFNSAGLGLVHALAHQPGATHNLPH
                     GVCNAILLPVIENFNRPNAIARFARIAQAMGVDTRDMSEEAASTEAINAIRALSHRVG
                     IPAGFSQLGITKEDIEGWLDKALADPCVPCNPRTASRDEVRELYLEAL"
     gene            35528..35842
                     /gene="rhaM"
                     /locus_tag="ACHKIK_001348"
     CDS             35528..35842
                     /gene="rhaM"
                     /locus_tag="ACHKIK_001348"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO9082555.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            36155..36601
                     /gene="frvA"
                     /locus_tag="ACHKIK_001349"
     CDS             36155..36601
                     /gene="frvA"
                     /locus_tag="ACHKIK_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO9082556.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFINQLRQGDADQVLALLNQTLSS"
     gene            36612..38063
                     /gene="frvB"
                     /locus_tag="ACHKIK_001350"
     CDS             36612..38063
                     /gene="frvB"
                     /locus_tag="ACHKIK_001350"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO9082557.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            38053..39123
                     /gene="frvX"
                     /locus_tag="ACHKIK_001351"
     CDS             38053..39123
                     /gene="frvX"
                     /locus_tag="ACHKIK_001351"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO9082558.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPQSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAKH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYFPNQKLVAALKSCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVSAFSQFRQVD"
     gene            39123..40871
                     /gene="frvR"
                     /locus_tag="ACHKIK_001352"
     CDS             39123..40871
                     /gene="frvR"
                     /locus_tag="ACHKIK_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO9082559.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(40921..>41956)
                     /gene="yiiG"
                     /locus_tag="ACHKIK_001353"
     CDS             complement(40921..>41956)
                     /gene="yiiG"
                     /locus_tag="ACHKIK_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO9082560.1"
                     /translation="RNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPEA
                     PVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQG
                     PTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTIN
                     EMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELKN
                     IEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGG
                     MNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALREYN
                     EMVDDYNSLR"
BASE COUNT        10160 a        10886 c        10886 g        10024 t
ORIGIN      
        1 ggtggaacgt ctgtgtgcgc ccgaagcggt ccaccagccc ggtcagtacc cggtacggcg
       61 gcagcggcgc aggcagcacc tcatccgctt ccggcacccg ctcacaccag ccgagcagcc
      121 accacggccc ctgcggactg tttgtcgcca gataacgatg cggacttaag cggagttctt
      181 ccggcagcgc ctgccagagt gcggccagcc ggtgaccttc atccagtttc gccacgccgc
      241 cgcgcaccag ccacagtgac tcgctgcggc tgtaaccgtc ctcaccggga aacaggtgct
      301 caaaatacag gcttctgccg ccgttatcac tgagtatcag tgtgttatcg cgcagctgta
      361 agcggatatc cgcaggcatt ttccagccgg ggccgaggct ccccaccggc gcgggcgttt
      421 ttgtccggta actgctgtag gtgcgggaga ggatgaacgg cagcgggccg ggcagggcga
      481 tgtcggtttc accgggaagg acctttgcac cgagcagcgg attgaccgga tggccggacg
      541 tcactccgcc ggggcacacc gaacaggcca cgccggtggg ggcaccaatg cgcaccccgg
      601 ctgaaccctg gacaatgcta ctgccatact gcgtcatgtc gccctgacgc gctgccggtt
      661 ttccgctcat cgtaaaatcc ttttgaaata aataagaacc agaagcctag cgccagtcaa
      721 caacaggaaa tgataatgag ttaacacccc ggaatttttc agaatttata aaaggcaaat
      781 tacaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga aatccggtct tatttgctgc
      841 ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc accggtagca acatcacgct
      901 gtttgccagt gaagaacggg tggcacttgc tgcacacgtc gaggttcagg tcatgaccaa
      961 cggtggagcg gattttcatt acgttaccgc aagagcagct agcagtaatt tcttcgtatt
     1021 tcgggtgaat atcttttttc atgggaaaac ctcggtttaa ggccgcgtcg ctcttccagc
     1081 cctaacgcca gacaccacgc gatgttaaaa gtatagcttc aatacgatca tttcgtacga
     1141 aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac tgatccgcac tcttctacgg
     1201 caatgtgtat actaacccac cgaatttcaa gtcaggatga tgctatgccc gtagcccacg
     1261 ttgccttgcc cgttccgctt cctcgtacct ttgactatct gctgccagaa ggcatgacgg
     1321 ttaaagctgg gtgtcgcgtg cgcgtgccgt ttggcaaaca gcaggagcgc atcgggattg
     1381 tggtatcagt tagcgatgcc agcgaactgc cgctcaatga gctaaaagcg gtagtcgaag
     1441 tgctggacgt tgagccggta tttactcact ccgtctggcg gttactgctc tgggcggcag
     1501 attactatca tcatccgatt ggcgatgtgc tgtttcatgc cttgccgatt ttactgcgcc
     1561 aggggcggcc tgcggcgaac gcgccgatgt ggtactggtt tgccactgaa caaggtcagg
     1621 cggtggatct taacagcctg aaacgttcgc caaagcaaca acaggcgctg gcggcgttac
     1681 ggcaaggcaa aatctggcgc gaccaggtcg cggaacttga atttaatgat gccgcgttgc
     1741 aggcgctgcg caaaaaaggt ctgtgtgatt tagcaagtga aacaccagag tttagcgact
     1801 ggcgaacgaa ctatgccgtt tctggtgagc ggttgcgatt gaataccgaa caagccaccg
     1861 ccgttggcgc aattcatagc gcggcagata ctttttctgc ctggctgctg gcgggcgtta
     1921 ccggttccgg taaaacggag gtttatctca gtgtactgga aaacgtgctc gctcagggca
     1981 aacaggcgct ggtgatggtg ccggaaatcg gcctgacacc gcaaactatc gcccgttttc
     2041 gtgaacgttt taatgccccc gtggaagttc tgcattccgg cctgaacgac agcgagcggc
     2101 tttcggcgtg gttgaaagcg aaaaatggcg aggcggcgat tgtgatcggc acccgctccg
     2161 cgctgtttac gccgtttaaa aatctcggcg tgattgtcat tgatgaagag cacgacagct
     2221 cctacaagca gcaggaaggc tggcgctatc atgcccgcga cctggcggtg tatcgtgcgc
     2281 atagcgagca aatcccgatt attcttggct ccgcaacgcc cgcgctggaa acgttatgca
     2341 acgtccagca gaaaaaatac cgcctgctgc gcctgacccg tcgggcaggg aatgcgcgtc
     2401 cggcaattca acatgtgctg gatttaaaag gtcagaaggt gcaggcaggt ctggctccgg
     2461 cgttaatcac ccgtatgcgc cagcatttac aggccaacaa tcaggttatc ctctttctta
     2521 atcgtcgggg ttttgcgcct gcgctgctgt gccacgactg cggctggata gccgaatgcc
     2581 cacgttgcga tcactactac acgctgcatc aggcgcaaca gcatttgcgc tgtcaccact
     2641 gtgacagtca gcgtccggtg ccgcgccagt gcccttcctg cggttccacg cacctggtcc
     2701 ccgtggggct gggcaccgaa cagcttgaac agacgcttgc gccgatgttc cccggcgtgc
     2761 ccatttcacg tatcgaccgc gataccacca gccgcaaagg ggcgctggaa cagcaactgg
     2821 cggaagtgca tcgcggcggc gcgaggatct tgattggcac gcaaatgctg gcgaaaggtc
     2881 accactttcc ggatgtgacg ctggtggcgt tactggatgt ggacggcgcg ctgttttctg
     2941 ccgattttcg ctcggcagaa cgtttcgccc agctttacac ccaggtcgcc ggtcgtgccg
     3001 ggcgtgcggg taaacagggc gaagtggtgc tgcaaacgca ccatccggaa catcctctgt
     3061 tgcaaacgtt gctctataaa ggctacgacg cctttgccga acaggcgctg gctgagcgac
     3121 gcatgatgca gctgccgccg tggaccagcc atgtgattgt gcgtgcggaa gatcataaca
     3181 atcagcatgc gccgctgttc ctgcaacaac tgcgtaatct gatcctttcc agcccgctgg
     3241 cagacgacaa actgtgggtt ctcggcccgg ttccggctct ggcacctaag cgtggtggcc
     3301 gctggcgctg gcagatcttg ttgcagcacc cttcccgcgt gcgcttgcaa cacatcatta
     3361 gtggtacgct ggcgcttatt aatacaatac cggattcccg caaggtgaaa tgggtgttgg
     3421 atgtcgatcc catcgaaggc taatgcgcaa acgttgcgag gcggatcgaa aaattcaata
     3481 ttcatcacac ttttcatgaa aattctgtaa ccgttttcac gcgctatctg ctaaaaatgt
     3541 tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt gccaggcgag gagtgagtgt
     3601 gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac gttgccctca aggcaaaagt
     3661 ctctacagcg actgtctccc gagcattaat gaatcccgat aaagtctccc aggccacccg
     3721 taatcgggtt gaaaaagcgg cccgggaagt gggttattta ccgcagccta tggggcgcaa
     3781 cgtcaagcgt aatgaatccc gcaccattct ggtgattgtc ccggatatct gcgatccctt
     3841 ctttagcgaa attattcgcg gtatcgaagt tacggcggca aatcacggat atctggtgct
     3901 gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc tttatcgatt tgatcatcac
     3961 caagcaaatt gatggcatgt tgctgctggg ttcaaggctg ccgtttgatg ccagcattga
     4021 ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa ttcgcaccgg aactggagct
     4081 acctacagtt catatcgaca atctgaccgc cgcatttgat gcagtaaatt atctatatga
     4141 gcaagggcat aaacggattg gctgtatagc cggtcccgaa gagatgccgc tgtgtcacta
     4201 ccgcctgcaa ggttacgttc aggcgctgcg tcgctgcggc attatggttg atccgcaata
     4261 catcgcccgt ggcgacttca ccttcgaagc cggaagcaaa gcgatgcagc agctgcttga
     4321 tcttccacaa ccgcctactg ctgtcttctg ccatagcgat gtgatggcgc tcggcgcact
     4381 ttctcaggca aaacgccagg ggctgaaagt cccggaagac ctttccataa tcggttttga
     4441 taacatcgac ctgacgcaat tttgtgatcc gccgctgaca accatcgcgc agccgcgtta
     4501 cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa atgcaggggc aacacgttgg
     4561 cagtggctct cgtttaatgg actgcgaact tatcatccgg ggatcaacac gcgcgttacc
     4621 ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc ccgccgctta agtaacatgg
     4681 cgggctgacg aacgaataaa tacagcgaaa cgatagtggc acaacgagat tatgtacgcc
     4741 gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc acggaaaaag caacgaaatc
     4801 tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc cgttcttgtg acctttatcg
     4861 gtggtctgta cttcattacg catcacaaga aagaagagtc cgagacgctg caaagccaga
     4921 aagtgaccgg taacggacta ccaccaaaac cagaagaacg ctggcgctac attaaagagc
     4981 tggaaagtcg ccagccgggt gtgcgtgcgc ccacagaacc ttctgccggt ggtgaagtga
     5041 aaacgccgga gcaactgaca ccagaacaac gtcagcttct tgaacaaatg caggctgata
     5101 tgcgccagca gccaacgcag ctggttgaag ttccgtggaa tgaacagacg ccggaacagc
     5161 gtcagcaaac gctacaacgc cagcgtcagg cgcagcaatt agcggagcag caacgcctgg
     5221 ctcagcagtc gcgtacgact gaacaaagct ggcagcagca gacgcgtacg tcgcaagccg
     5281 cgccggtaca ggcgcaaccg cgccagtcta aaccggcttc tactcagcag ccgtaccagg
     5341 atctgctgca aacccctgcg cacacgaccg cgcagtcgaa gccgcagcaa gctgcgccgg
     5401 ttgctcgtgc cgctgacgcg ccaaaaccga cggcggagaa aaaagacgaa cgccgctgga
     5461 tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga gacggtacgt gctcagctgg
     5521 cgttcgaagg atttgactcg aaaatcacca ccaacaatgg ctggaatcgt gtggtcattg
     5581 gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa tcggttgaag atggcgggtc
     5641 atacaaactg cattcggctc gccgccgggg gttgaaaccc tcaaaatccc ccccatctat
     5701 aattgcatta tgccccgtac ttttgtacgg ggtttgtact ctgtattcgt aaccaagggg
     5761 tcagctcgtg acaactatag taagcgtacg ccgtaacggc catgtggtca tcgctggtga
     5821 tggtcaggcc acgttgggca ataccgtaat gaaaggcaac gtgaaaaagg tccgccgtct
     5881 gtacaacgac aaagtcatcg cgggctttgc gggcggtact gcggatgctt ttacgctgtt
     5941 cgaactgttt gaacgtaaac tggaaatgca tcagggccat ctggtcaaag ccgccgttga
     6001 gctagcgaaa gactggcgta ccgatcgcat gctgcgcaaa cttgaagcac tgctggcagt
     6061 cgcggatgaa actgcatcgc ttatcatcac cggtaacggt gacgtggtgc agccagaaaa
     6121 cgatcttatt gctatcggct ccggcggccc ttacgcccag gctgcggcgc gtgcactgtt
     6181 agaaaatact gaactgagtg ctcgtgaaat tgctgaaaag gcgttggata ttgcaggcga
     6241 catttgcatc tataccaacc atttccacac catcgaagaa ttaagctaca aagcgtaagg
     6301 atctcccatg tctgaaatga ccccacgcga aatcgtcagc gaactggata agcacatcat
     6361 cggccaggac aacgccaagc gttctgtggc gattgctctg cgtaaccgct ggcgtcgcat
     6421 gcagctcaac gaagagctgc gccatgaagt gaccccgaaa aatatcctga tgatcggccc
     6481 gaccggtgtc ggtaaaactg aaatcgcccg tcgtctggct aagctggcga atgcgccgtt
     6541 catcaaagtt gaagcgacca aattcaccga agtaggctac gtcggtaagg aagtggattc
     6601 tattattcgc gatctgaccg atgccgccgt gaaaatggta cgcgtccagg ctatcgagaa
     6661 aaaccgttat cgcgctgaag aactggcaga agaacgtatt ctcgacgtgc tgatcccacc
     6721 tgctaaaaac aactggggac agaccgaaca gcagcaggaa ccgtccgctg ctcgtcaggc
     6781 attccgcaaa aaactgcgtg aaggccagct tgatgacaaa gaaatcgaga tcgatcttgc
     6841 cgcagcaccg atgggcgttg aaattatggc tcctccaggc atggaagaga tgaccagcca
     6901 gctgcagtcc atgttccaga acctgggcgg ccagaagcaa aaagcgcgta agctgaaaat
     6961 caaagacgcc atgaagctgc tgattgaaga agaagcggcg aaactggtga acccggaaga
     7021 gctgaagcaa gacgctatcg acgctgttga gcagcacggg atcgtgttta tcgacgaaat
     7081 cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat gtttctcgtg aaggcgttca
     7141 gcgtgacctg ctgccgctgg tagaaggttg caccgtttcc accaaacacg ggatggtcaa
     7201 aactgaccac attctgttta tcgcttctgg cgcgttccag attgcgaaac cgtctgacct
     7261 aatcccggaa ctgcaaggtc gtctgccaat ccgcgttgaa ctgcaggcac tgaccaccag
     7321 cgacttcgag cgtattctga ccgagccgaa tgcctctatc accgtgcagt acaaagcact
     7381 gatggcgact gaaggcgtaa atatcgagtt taccgactcc ggtattaagc gcatcgcgga
     7441 agcggcatgg caggtgaacg aatctaccga aaacatcggt gctcgtcgtt tacacactgt
     7501 tctggagcgt ttaatggaag agatttccta cgacgccagc gatttaagcg gtcaaaatat
     7561 cactattgac gcagattatg tgagcaaaca tctggatgcg ttggtggcag atgaagatct
     7621 gagccgtttt atcctataat cgcgttcaat cattttcatc attgtttgat ggggctgaaa
     7681 ggccccattt ttattggcgc gtattatgac tgaacaacaa attagccgaa ctcaggcgtg
     7741 gctggaaagt ttacgaccta aaaccctccc cctcgccttt gctgcaatta tcgtcgggac
     7801 ggcgctggca tggtggcaag gtcacttcga tccgctggtc gccctgctgg cactaattac
     7861 cgccgggcta ttacagatcc tttctaacct cgccaatgat tacggcgatg cggtaaaagg
     7921 cagcgataaa cctgaccgca ttgggccgct acgcggcatg caaaaagggg tcattaccca
     7981 gcaagagatg aaacgggcgc tcattattac cgtcgtgctc atctgtctct ccgggttggc
     8041 actggttgca gtggcatgcc atacgctggc cgattttgtc ggtttcctga ttcttggcgg
     8101 gttgtcgatc attgccgcta tcacctacac cgtgggcaat cgtccttatg gttatatcgg
     8161 tctgggtgat atttccgtac tggttttctt tggctggttg agtgtcatgg ggagctggta
     8221 tttacaggct catacattga ttccggcact gatccttccg gcgaccgcat gcggcctgct
     8281 ggcaacggca gtactgaata ttaataacct gcgtgatatc aatagcgacc gcgaaaatgg
     8341 caaaaacacg ctggtggtgc gcttaggtga agtgaacgcg cgtcgttatc atgcctgcct
     8401 gctgatgggc tcgctggtgt gtctggcgct gtttaatctc ttttcgctgc atagcctgtg
     8461 gggctggctg ttcctgctgg cggcaccatt actggtgaag caagcccgtt atgtgatgcg
     8521 ggaaatggac ccggtggcga tgcgaccaat gctggaacgt actgtcaagg gagcgttact
     8581 gactaacctg ctgtttgttt tagggatatt cctaagccag tgggcagcat aactgacaaa
     8641 tatcaattaa caattgatga ttttgccaac agcccacata gcgcgatata ctgaaaattc
     8701 tcgcagcaac tgaatgttaa gcctatgaaa tacgatactt ccgagctttg tgacatctat
     8761 caagaagatg ttaacgtcgt ggaaccgctg ttctccaact ttggcggacg ggcgtcgttt
     8821 ggcggacaaa taatcacggt aaaatgtttc gaggacaacg ggttgctgta cgatctgctc
     8881 gaacagaatg gccgtggtcg tgttcttgtc gtcgatggcg gtggttctgt tcgtcgcgca
     8941 ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg aatgggaagg tctggtcatt
     9001 tacggcgcgg tgcgtcaggt agatgacctg gaagagttgg atatcggcat ccaggcgatg
     9061 gcggcaattc cggttggtgc cgctggcgaa ggcattggcg aaagcgatgt ccgcgtcaat
     9121 tttggcggtg tcaccttctt ctccggcgac catctttatg ccgacaatac cgggattatt
     9181 ctttctgaag atccgctgga tattgaatga tagaaaaggc accgcaaggt gccttttttc
     9241 tgcgttacct gttggcctac acagtaaaga aattacgcgg aagatgaagc gtaatcagac
     9301 ctcttccatg cgacccagca gggcctgcag acgttcctgc cagccgttct gctgttcttt
     9361 cagatggttg ttctcacgct ccagctcttc gcgctgatgc tgggcatttt gaacttcctg
     9421 cgacagtgag ttgttttttt ctttcagctc ttcgatttcc atctgcaaca gagtgatggt
     9481 atcaatcgcc tgctgtactt ttgcttccag tttctcaaac acttctaatg acattgtcat
     9541 acctctcctg aattgcaagg cgttgatgga taaaaatcct cgtcccgatt accggtgacg
     9601 ccttaataaa tacgagcgca ctttagttag ctccgattgt atgaagccgc gccatcgctg
     9661 tccagcggca cgccttgcag attacggttt gccacacttt tcatccttct cctggtgaca
     9721 taatccacat caatcgaaaa tgttaataaa tttgttgcgc gaatgatcta acaaacatgc
     9781 atcatgtaca atcagatgga ataaatggcg cgataacgct cattttatga cgaagcacac
     9841 acattttaag ttcgatattt ctcgcttttg ctcgttaacg ataagtttac agcatgccta
     9901 caagcatcgt ggaggtccgt gactttcacg catacaacaa acattaactc ttcaggatcc
     9961 gattatgagt caaacatcaa ccttgaaagg ccagtgcatt gctgaattcc tcggtaccgg
    10021 gttgttgatt ttcttcggtg tgggttgcgt tgcagcacta aaagtcgctg gtgcgtcttt
    10081 tggtcagtgg gaaatcagtg tcatttgggg actgggggtg gcaatggcca tctacctgac
    10141 cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc attgcattgt ggctgtttgc
    10201 ctgtttcgac aagcgcaaag ttattccttt tatcgtttca caagttgccg gcgctttctg
    10261 tgctgcggct ttagtttacg ggctttacta caatttattt ttcgacttcg agcagactca
    10321 tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct ggcactttct ctacttaccc
    10381 taatcctcat atcaattttg tgcaggcttt cgcagttgag atggtgatta ccgctattct
    10441 gatggggctg atcctggcgt taacggatga tggcaacggt gtaccacgcg gccctttggc
    10501 tcccttgctg attggtctac tgattgcggt cattggcgca tctatgggcc cattgacagg
    10561 ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc tttgcctggc tggcgggctg
    10621 gggcaatgtc gcctttaccg gcggcagaga cattccttac ttcctggtgc cgcttttcgg
    10681 ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc aaactgattg gtcgccattt
    10741 gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca actccttcag aacaaaaagc
    10801 ttcgctgtaa tatgactacg ggacaattaa acatgactga aaaaaaatat atcgttgcgc
    10861 tcgaccaggg caccaccagc tcccgcgcgg tcgtaatgga tcacgatgcc aatatcatta
    10921 gcgtgtcgca gcgcgaattt gagcaaatct acccaaaacc aggttgggta gaacacgacc
    10981 caatggaaat ctgggccacc caaagctcca cgctggtaga agtgctggcg aaagccgata
    11041 tcagttccga tcaaattgca gctatcggta ttacgaacca gcgtgaaacc actattgtct
    11101 gggaaaaaga aaccggcaag cctatctata acgccattgt ctggcagtgc cgtcgtaccg
    11161 cagaaatctg cgagcattta aaacgtgacg gtttagaaga ttatatccgc agcaataccg
    11221 gtctggtgat tgacccgtac ttttctggca ccaaagtgaa gtggatcctc gaccatgtgg
    11281 aaggctctcg cgagcgtgca cgtcgtggtg aattgctgtt tggtacggtt gatacgtggc
    11341 ttatctggaa aatgactcag ggccgtgtcc atgtgaccga ttacaccaac gcctctcgta
    11401 ccatgttgtt caacatccat accctggact gggacgacaa aatgctggaa gtgctggata
    11461 ttccgcgcga gatgctgcca gaagtgcgtc gttcttccga agtatacggt cagactaaca
    11521 ttggcggcaa aggcggcacg cgtattccaa tctccgggat cgccggtgac cagcaggccg
    11581 cgctgtttgg tcagttgtgc gtgaaagaag ggatggcgaa gaacacctat ggcactggct
    11641 gctttatgct gatgaacact ggcgagaaag cggtgaaatc agaaaacggc ctgctgacca
    11701 ccatcgcctg cggcccgact ggcgaagtga actatgcgtt ggaaggtgcg gtgtttatgg
    11761 caggcgcatc cattcagtgg ctgcgcgatg agatgaagtt gattaacgac gcctacgatt
    11821 ccgaatattt cgccaccaaa gtgcaaaaca ccaatggtgt gtatgtggtt ccggcattta
    11881 ccgggctggg tgcgccgtac tgggacccgt atgcgcgcgg ggcgattttc ggtctgactc
    11941 gtggggtgaa cgctaaccac attatacgcg cgacgctgga gtctattgct tatcagacgc
    12001 gtgacgtgct ggaagcgatg caggccgact ctggtatccg tctgcacgcc ctgcgcgtgg
    12061 atggtggcgc agtagcaaac aatttcctga tgcagttcca gtccgatatt ctcggcaccc
    12121 gcgttgagcg cccggaagtg cgcgaagtca ccgcattggg tgcggcctat ctcgcaggcc
    12181 tggcggttgg cttctggcag aacctcgacg agctgcaaga gaaagcggtg attgagcgcg
    12241 agttccgtcc aggcatcgaa accactgagc gtaattaccg ttacgcaggc tggaaaaaag
    12301 cggttaaacg cgcgatggcg tgggaagaac acgacgaata atgtaaatgc cgaatgaagc
    12361 gtttatgccg catccggtag tcccgaaacg tgcgggggca accccgcaca catcaataat
    12421 ccctcccttc ccctgtgcta cacttcgcgc cattccttac tgcttagagt ttgctatgag
    12481 acgagaactt gccatcgaat tttcccgcgt caccgaatca gcggcgctgg ctggctacaa
    12541 atggttagga cgcggcgata aaaacaccgc ggacggcgcg gcggtaaacg ccatgcgtat
    12601 tatgctcaac caggtcaaca ttgacggcac catcgtcatt ggtgaaggtg aaatcgacga
    12661 agcaccgatg ctctacattg gtgaaaaagt cggtactggt cgcggcgacg cggtagatat
    12721 tgctgttgat ccgattgaag gcacgcgcat gacggcgatg ggccaggcta acgcgctggc
    12781 ggtgctggca gtaggcgata aaggctgctt cctcaatgcg ccggatatgt atatggagaa
    12841 gctgattgtc gggccgggag ccaaaggcac cattgatctg aacctgccgc tggcggataa
    12901 cctgcgcaat gtagcggcgg cgctcggcaa accgttgagc gaactgacgg taacgattct
    12961 ggctaaacca cgccacgatg ccgttatcgc tgaaatgcag caactcggcg tacgcgtatt
    13021 tgctattccg gatggcgacg ttgcggcctc aattctcacc tgtatgccag acagcgaagt
    13081 tgacgtgctg tacggtattg gtggcgcgcc ggaaggcgta gtttctgcgg cggtgatccg
    13141 cgcattagat ggcgacatga acggtcgtct gctggcgcgt catgacgtca aaggcgacaa
    13201 cgaagagaat cgtcgcattg gcgagcagga gctggcacgc tgcaaagcga tgggcatcga
    13261 agccggtaaa gtattgcgcc tgggcgatat ggcgcgcagc gataacgtca tcttctctgc
    13321 caccggtatt accaaaggcg atctgctgga aggcattagc cgcaaaggca atatcgcgac
    13381 taccgaaacg ctgctgatcc gcggcaagtc acgcaccatt cgccgcattc agtccatcca
    13441 ctatctggat cgcaaagacc cggaaatgca ggtgcacatc ctctgattga tttgatcgat
    13501 tgagccttcc agtccttcgg gactggaatt tttttgttcg gagaacgaag ataaggcaag
    13561 tcaatcaaaa caggagaaaa acatggctga ttgggtaaca ggcaaagtca ctaaagtgca
    13621 gaactggacc gacgccctgt ttagtctcac cgttcacgcc cccgtgcttc cgtttaccgc
    13681 cgggcaattc accaagcttg gccttgaaat cgacggcgaa cgcgtccagc gcgcctactc
    13741 ctatgttaac tcgcccgata atcccgatct ggagttttac ctggtcaccg tccccgatgg
    13801 caaattaagc ccacgattgg cggcactgaa accaggcgat gaagtgcagg tggttagcga
    13861 agcagctggc ttctttgttc tggatgaagt accggactgc gaaacgctat ggatgctggc
    13921 aaccggtaca gcgattggcc cttatttatc gattctgcaa ctaggcaaag atttagatcg
    13981 cttcaaaaat ctggtcctgg ttcacgccgc acgttatgcc gccgacttaa gctatttgcc
    14041 actgatgcaa gaactggaaa aacgctacga agggaaactg cgcattcaga cggtggtcag
    14101 tcgggaaacg gcagcggggt cgctcaccgg gcggataccg gcgttaattg aaagtgggga
    14161 actggaaagc gcgattggcc tgccgatgaa taaagaaacc agccatgtga tgctgtgcgg
    14221 caatccacag atggtgcgcg atacacaaca gttgctgaaa gagacccggc agatgacgaa
    14281 acatttacgt cgccgaccgg gccatatgac agcggagcat tactggtaag cggttactta
    14341 tcgataaacg gcacgatgag caaatccgca ctcatcttat tgatcatccc gcgatatgcc
    14401 ggcatcaaac ggttgataaa tgagtgatga tgaccacaga caaggaggtc gcactgctct
    14461 ttttgcataa tttccagcag tgtttccggc atttctccgc gttcaatacg cagttttgtc
    14521 ttcggccatt gaatattttt cgtcagttta tacagcttgt tatccgactt attcttcaac
    14581 aattgaagaa tatcttctgt tgcagggaag tagatacccg gatacaactc gcttaagcca
    14641 tcatcaatat gaattaacgt caggtgagcg tcattatgtc tggcgagctc cagagcttta
    14701 ttcaccagta aggcatcttc ttcattcccg gaaattgcca cgccaatgtg tttataagcc
    14761 atgtttaact ccttctaaag caactccatc aagctaacga acgcagtgat aactcaaaaa
    14821 taatcatctc ggcctggcat gagaaagtga aggccgcatc aagctcaact tgcccctcca
    14881 gatccttaat cttgtaggcc acgctggccc cttcatctgc agaggatgca gcggcggcaa
    14941 gctctttcag tttggtcata aaagactctg cttcttcgcg ggtggcaaaa aagcgcgaaa
    15001 acttactggt acagttgtcg ttatcaatca ccgtaccgat atcaatcgca catcctttag
    15061 tactgcattt atctacgaca tctttcatag ggcacctctg tatattcgcc tctctgtcga
    15121 ttacgactta agaataatgc gccgttaagg gaagaacaaa caagtaatga tcttatggat
    15181 taccattttt tttgatattg cttaaggtga agagttcaga aggattttcc gggtattgtc
    15241 atttgcatcg tggaaataac aatcccggaa aaaaatagca gtaagagata tattaaaggt
    15301 taattacttg atttattgtc ggctttatac ttcacatcct gagtatcttt accatattta
    15361 ttttcgcctt gtgtgccaac aaacgcacca agatcgataa gcatcatcac cagaatcaac
    15421 gtcgggacaa aacgccccac cgcccattgc cagacacccg gtaaaatcgc ccagttaccc
    15481 gccagcagca tccacgccac aatcatcaga aatgcccatg cgccggaacg cccgcgatca
    15541 tgcaagcgct taacagttac tgccgctgtt ggccaaagca agcacacaag gcaaaacgcc
    15601 gcggtctgaa tatcgagtaa atttttaccc gccagtgaga aaagcaccag catgcctgcg
    15661 aaccacaggc ctatccaaat ccagaaatca cggcgtccaa tacgcccttt aaatgagaat
    15721 aaccattgct gtatggtcat gtaagttcct tgatggttgt cttttccagg attctaccct
    15781 tttgacaagg gcgacagata tcgttttaat cggagccagt cataacaaaa ggtactgtca
    15841 atgaagccag ggtgtacgct gttttttctc ttatgttctg cattaaccgt tacaacaacg
    15901 gcgcatgcac aaacaccaga tacggcaacg accgcgcctt atctgctggc tggagcccct
    15961 actttcgatc tctccatcag ccagtttcga gaagacttta acagccagaa tcctagcctg
    16021 ccactgaacg aatttcgtgc catcgacagc agtcccgaca aagccaatct cactcgtgct
    16081 gcaagtaaaa ttaacgagaa cttgtatgct tctacagcgc tggagcgcgg taccttaaaa
    16141 atcaaaagca ttcaaatgac ctggctaccc atccaggggc cagagcaaaa agcagcgaaa
    16201 gcgaaagctc aggaatacat ggcagcagtg atccgcacac tcaccccatt aatgaccaaa
    16261 acacaaagcc agaaaaaact gcagtcgcta ctaacggcgg ggaaaaacaa acgttattac
    16321 accgagacag aaggtgcact gcgttatgtt gtcgcggaca acggcgaaaa ggggctgacc
    16381 ttcgctgttg aaccgattaa gctggcgcta tctgaatcgc ttgaaggttt gaataaatga
    16441 caaaaagcaa agcctttgtg ccgatgaatc tctatactgt ttcacagacc tgctgccctg
    16501 cggggcggcc atcttccttt attcgcttat aagcgtggag aattaaaatg cgacatcctt
    16561 tagtgatggg taactggaaa ctgaacggca gccgccacat ggttcacgag ctggtttcta
    16621 acctgcgtaa agagctggca ggtgttgctg gctgtgcggt tgcaatcgca ccaccggaaa
    16681 tgtacatcga tatggcgaag cgcgaagctg aaggcagcca catcatgctg ggtgcgcaaa
    16741 acgtggacct gaacctgtcc ggcgcattca ccggtgaaac ttctgccgct atgctgaaag
    16801 acatcggcgc tcagtacatc atcatcggcc actctgaacg tcgtacttac cacaaagagt
    16861 ctgacgaact gattgcgaaa aaattcgcgg tgctgaaaga gcagggcctg actccggttc
    16921 tgtgcatcgg tgaaaccgaa gctgaaaacg aagcgggcaa aactgaagaa gtttgcgcac
    16981 gtcagatcga cgcggtactg aaaactcagg gtgctgcggc attcgaaggt gcggttatcg
    17041 cttacgaacc tgtatgggca atcggtactg gcaaatctgc aactccggct caggcacagg
    17101 ctgttcacaa attcatccgt gaccacatcg ctaaagttga cgctaacatc gctgaacaag
    17161 tgatcattca gtacggcggc tctgtaaacg cgtctaacgc tgcagaactg tttgctcagc
    17221 cagatatcga cggcgcgctg gttggcggtg cttctctgaa agctgacgct ttcgcagtga
    17281 tcgttaaagc tgcagaagcg gctaaacagg cttaagtctg acaggtgccg gatttcatat
    17341 ccggcactta ctttccttaa ctcctcgcct taacgcaaaa tctcacactc atgatcctgg
    17401 atttcctcgg ctgaagcacg gttcagcgtc agtagatttc gttgtgtcgc cagcaacaca
    17461 aacgaattat cactctgccg taccatcgcc agcccgtagc ttcccatatg ttcccgcgcc
    17521 tcaggcactt cttctgcaag catcataaat gggctgcgtt gtaccagttc gctttccgtt
    17581 acccgacgcg ccaggtattc atgcccgcgc aaaccacctg gcagtggcaa ccagcggctg
    17641 ctgatgttcg ccagattttt atccagttgt tcgcgcacat caggacgaat acaagagata
    17701 tgaatatgaa aatggttttg cgtacgcccg gtgcgggagt tgatcgccaa agaaaccgcg
    17761 cgatcgggaa ccggctggcc gtattttttg ctcataaaat cacgcgcctg ccaggccaac
    17821 caaaagaagt tcggcgttga aggatcggtc aacaaaggac tttcagtacc gttaatacga
    17881 tacgttggca tcaacagata ttgcagtgga ccattaagat cttttaaaac cacgtatccg
    17941 gcattgggtt tgacttccgc acatggcgaa ggattttgat tttgctgctg attgggcaaa
    18001 cattcctcaa ggacaatctt acgtaatgta tccgactctt caccggttaa tttccagtaa
    18061 ccaataccgg cagccacaac ggcgataact atcatcacca aaaaaagaag acccgctttt
    18121 ttcatctttt tttccctgta cctcaaagag atgcaagggt aacgcaaaat cgtgacaaat
    18181 aaaaaacccg gtcgcgagaa accaccgggt ttgataatta tcctggggaa atcagcgttt
    18241 gctgatctga tcgaacgtac cgccgttagc aaaatgctct ttttgcgctt tcgtccagcc
    18301 gccgaactct tcatcaatgg tgaataactt cagctttgga aacgcatttt cgtacttttt
    18361 cgctacttca gcgtcgcgcg gacggtagta gtttttcgcg gcaatttcct gaccttctgg
    18421 cgagtagaga tatttcaggt aggcttccgc cacctctttg gtgccttttt tctcgaccac
    18481 tttatcgacc accgacacgg ttggctctgc gaggatagac tcactcggcg tgacgatttc
    18541 gaatttatct ttccccagtt cattcgctac cagcagagct tcgttttccc aggcgatcag
    18601 tacatcgcca atcccgcgct cgacaaaggt gttagtggag ccgcgcgcgc cagaatccag
    18661 aacttcgacg tttttataca gtgcccgaac aaaatcctgt gcttttgcct gatcgttgtt
    18721 gttgtgatgc agcgcgtagc cccaggcagc caggtagttc cagcgtgcgc caccagagct
    18781 tttcggatta ggcgtgatca ccgaaacacc cggtttaatc agatcgttcc agtcatggat
    18841 ctgcttcgga ttgcccttac gcaccaggaa aacaatggtg gaagtgtacg gtgcggagtt
    18901 atccggcaga cgtttgatcc actctttatc aatccgcccg cgttccgcaa tcgcgtccac
    18961 gtcataggcc agagccagcg tgacaacatc agcttcaata ccgttgatta ccgacgtcgc
    19021 ttgtttacct gaaccaccgt gtgactgacg aatcaccacg ttatcaccag tttgctgttt
    19081 ccagtgggcg ctgaatgcct tgttgtactg ttcgtacaat tcgcgcgttg gatcatatga
    19141 aacgttaaga agctgaatat cctttgccat aacgttggtt gccgccagca aaaatgttaa
    19201 ccctacgccc cacttgttca tcgcccgact ctcttatgtt gtgttgtgat gagcaaagcg
    19261 tgccagaagg ttaaccaaac attaaagaat aaaaaaagat tggctataac ttgcgggtat
    19321 atgttggggg ttaaaaaggc ggaagaaatc cgcctcatat tgctgacaaa gtgcgctttg
    19381 tccatgccgg atgcggcgcg aacgccttat ccggcctaca aaagtttgca aattcaataa
    19441 attgcagaat tcatgtaggc ctgataagcg aagcgcatca ggcatttttg cttctgtcat
    19501 cggtttcagg gtaaaggaat ctgccttttt ccgaaatcat taatacagtt ttttcgcgca
    19561 gtccagccag tcacctttga acggacgctt catgttttcg atagcgtcga tgatgtcgtg
    19621 gtgaaccagc tgttcgttct ggatacctac acaacgaccg ccgtaacctg ccagcagcag
    19681 atcgatagcg taagcgccca tacgggaagc cagaatacgg tcgtaaggca ccggagaacc
    19741 accgcgctgg atgtggccca gcacagttgc gcgggtttca cgaccggttt ctttctcgat
    19801 gaaatgcgcc agttcgtcaa catcacacat atgttcggta atcgccacga tcgcgtgttt
    19861 tttacctttc gcgatacccg ctttgatttc gtttaccagg tcttcacggc tgaattcaac
    19921 ttccggaacc acaacgaatt cacagccacc ggcaatggcc gcagccaacg tcagatcgcc
    19981 acaataacgg cccatcactt ccaccacgga aatacgctgg tgagaagaag aggtgtcacg
    20041 cagacggtcg atcgcttcta caacggtgct cagcgcagtg aagaaaccga tagtgtagtc
    20101 agtgcctttg atgtcgttgt cgatagtgcc cggcagaccg atgcacggga agcccatttc
    20161 ggtcagacgc attgcaccca tgtaggaacc gtcaccgccg ataaccacca gcgcgtcgat
    20221 cccacgtttt ttcaggtttt cgatagccac ggcgcggatg ttctcgtcgc ggaattccgg
    20281 gaagcgcgca gaaccgagga acgtaccgcc acggttgatc atgtcagaaa cgctgtaacg
    20341 gtctagctgt accatacggt cttcatacag acccagatag ccgtcataaa tgcccattac
    20401 ttccagacct tctgtcagcg cagaacgaac aaccccgcga attgcggcgt tcatgcctgg
    20461 cgcatcaccg ccgcttgtca acacaccgat tttcttaatc atgactacct ctgaactttg
    20521 gaatgcaaaa taaaatctgt tgccggaagt cttcttgcac atcgaagtga tccaacgaat
    20581 gtgcaaatag tataacaatc acttcctgct gaattgattc aggtcaggcc aaatggcggt
    20641 attttataca caaaatgcgg gtctggctct cttttatact gattatgaaa gcatagaccg
    20701 tttaccctcc ctgggtacga cggaacaggg gtcctgatgg ataattacat ccgatcccgg
    20761 aaaacgccgt aaaatagcct gctctacctg atccgccacc atatgtgcct gaaccaaagg
    20821 cagagagtct tccatttcca aatgaatctg aataaagcgg gtcggccctg actgccgcgt
    20881 gcgaagatcg tgagcgccgc taacacccgg ccaggaagtc acgatatcaa taatttcttg
    20941 ccgttcctca tcaggcaatg cgcgatccag taatgactgt accgcctcat atcccatgcg
    21001 taacgcgcta tataaaatat agatgccgat tcccaatgca aacagagcat cggcgcgatg
    21061 ccagccgtac caggacaacc ccagcgccag cagaattgcg ccgttcatca taacatcaga
    21121 ctggtaatgt agcatatcag cccgcaccgc ctggctttgc gtccggcgca ccacccaacg
    21181 ctgaaacgag acaaggataa tcgtacaaat tagcgccaca attgtcacga taaccccgac
    21241 gcctggatct gtcatcggtg ttggagatat cagatgttga atacccgtca aaaacaggaa
    21301 tagtgccgaa ccggagataa acatactttg cgccagcgcc gcgagggact ctgctttacc
    21361 gtgaccaaac gagtgattat cgtcggcagg ttgcagggaa tatcgcacca ccagtaaatt
    21421 cgtcaacgac gcgccgatat ccaccagcga atccaccagc gcggcgagaa tactcaccga
    21481 cccggtatac caccatgcaa aaattttaat cagcaatagc aacgaagcca tcgccgtcgc
    21541 agcaatcgcc gcccgactga ccagccgtcc ataagattga ttcataaata ctcccgctat
    21601 caactgacgc tagtataacg gaagcaaatc atctgcaatg cattaagcag caggcaaatt
    21661 gaggataaaa aaaaccccca catcatgtgg gggaagacag ggatggtgtc tatggcaagg
    21721 aaaacagggt ttactactgg gaacgtgagt tgctactact caatagcttc aacgatgaac
    21781 ttttttgcca ttgcgtcacg tcacgcaact gctccattcg ttgttgatgt ttctcgttta
    21841 aaaccgcttg ctgctccggc gttaacaggc gatacatttg gttgcggact tttgccatct
    21901 caacctgacg agcaatttgc tcattcgcca ttttttctgc ctgtgcgcgc acagcgtttt
    21961 catcaaaatt ttctgcggtg acaaggcgat gcattgtctc cagttcgcta acattaacag
    22021 gaggctgttc gtgccgggcc tgttgcataa gatctcgcat ctgctgacgc tgatgttcgg
    22081 ttaaacttat gccgtcgaac atatggctct gcgtactgcg ctgcgtaagt tcttcacccg
    22141 gatgccagtt atcgcctgaa ccgacttcag cagcgtggct taatgaactg actgccagcg
    22201 ttgaggccat gacggcagcg gtaactatgc gcatcatttg ctcccaaaat ctttctgtcg
    22261 cgattcaacg atagagagtt tacgattcag gctgcaaaca tgcgtcaggg ggtgtaaaac
    22321 aacgtaaagt catggattag cgacgtctga tgacgtaatt tctgcctcgg aggtatttaa
    22381 acaatgaata aaatcctgtt agttgatgat gaccgagagc tgacttccct attaaaggag
    22441 ctgctcgaga tggaaggctt caacgtgatt gttgcccacg atggggaaca ggcgcttgat
    22501 cttctggacg acagcattga tttacttttg cttgacgtaa tgatgccgaa gaaaaatggt
    22561 atcgacacat taaaagcact tcgccagaca caccagacgc ctgtcattat gttgacggcg
    22621 cgcggcagtg aacttgatcg cgttctcggc cttgagctgg gcgcagatga ctatctcccg
    22681 aaaccgttta atgatcgtga gctggtggca cgtattcgcg cgatcctgcg ccgttcgcac
    22741 tggagcgagc aacagcaaaa caacgacaac ggttcaccga cactggaagt tgatgcctta
    22801 gtgctgaatc caggccgtca ggaagccagc ttcgacgggc aaacgctgga gttaaccggt
    22861 actgagttta ccctgctcta tttgctggca cagcatctgg gtcaggtggt ttcccgtgaa
    22921 catttaagcc aggaagtgtt gggcaaacgc ctgacgcctt tcgaccgcgc tattgatatg
    22981 cacatttcca acctgcgtcg taaactgccg gatcgtaaag atggtcaccc gtggtttaaa
    23041 accttgcgtg gtcgcggcta tctgatggtt tctgcttcat gataggcagc ttaaccgcgc
    23101 gcatcttcgc catcttctgg ctgacgctgg cgctggtgtt gatgttggtt ttgatgttac
    23161 ccaagctcga ttcacgccag atgaccgagc ttctggatag cgaacagcgt caggggctga
    23221 tgattgagca gcatgtcgaa gcggagctgg cgaacgatcc gcccaacgat ttaatgtggt
    23281 ggcggcgtct gttccgggcg attgataagt gggcaccgcc aggacagcgt ttgttattgg
    23341 tgaccaccga aggccgcgtg atcggcgctg aacgcagcga aatgcagatc attcgtaact
    23401 ttattggtca ggccgataac gccgatcatc cgcagaagaa aaagtatggc cgcgtggaac
    23461 tggtcggtcc gttctccgtg cgtgatggcg aagataatta ccaactttat ctgattcgtc
    23521 cggccagcag ttctcaatcc gatttcatta acttactgtt tgaccgcccg ctattactgc
    23581 tgattgtcac catgttggtc agtacgccgc tgctgttgtg gttggcctgg agtctggcaa
    23641 aaccggcgcg taagctgaaa aacgctgccg atgaagttgc ccagggaaac ttacgccagc
    23701 acccggaact ggaagcgggg ccacaggaat tccttgccgc aggtgccagt tttaaccaga
    23761 tggtcaccgc gctggagcgc atgatgacct ctcagcagcg tctgctttct gatatctctc
    23821 acgagctgcg caccccgctg acgcgtctgc aactgggtac ggcgttactg cgccgtcgta
    23881 gcggtgaaag caaggaactc gagcgtattg aaaccgaagc gcaacgtctg gacagcatga
    23941 tcaacgatct gttggtgatg tcacgtaatc agcaaaaaaa cgcgctggtt agcgaaacca
    24001 tcaaagccaa ccagttgtgg agtgaagtgc tggataacgc ggcgttcgaa gccgagcaaa
    24061 tgggcaagtc gttgacagtt aacttcccgc ctgggccgtg gccgctgtac ggcaatccga
    24121 acgccctgga aagtgcgctg gaaaacattg ttcgtaatgc tctgcgttat tcccatacga
    24181 agattgaagt gggctttgcg gtagataaag acggtatcac cattacggtg gacgacgatg
    24241 gtcctggcgt tagcccggaa gatcgcgaac agattttccg tccgttctat cggaccgatg
    24301 aagcacgcga tcgtgaatct ggcggtacag gattggggct ggcgattgtt gaaaccgcca
    24361 ttcagcagca tcgtggctgg gtgaaggcag aagacagccc gctgggcggt ttacggctgg
    24421 tgatttggtt gccgctgtat aagcggagtt aaactccgca tttgtaggca ggataaggcg
    24481 tttacgccgc atccggcatt tgagcaggat aaggcgttta cgccgcatcc ggcatttgag
    24541 caggataagg cgtttacgcc gcatccggca tttgagcagg atgcctgatg cgacgctgat
    24601 agcgtcttat caggcctaca ctccgggggt tattttcccc acaaccggcg ggaaaaatct
    24661 tcgatcttgc cgctcagtcg gcgcttttgc atcgtccgcg tccagctttt cgataacccg
    24721 gcggcggaga gtaaacggtg atactgatcg tcatcaaacg gcatatgcca tgcgatggcg
    24781 gcagcctcct gcacggtcac atcgctgaca cgggaaacca actccagcgg cgcgtccgca
    24841 gacactttcc ctggcgctat cacgctatac aaccacccca ccttacctgt gttttgcatc
    24901 aactgcgcta tatcgctgat atcaaaatgg taattgagtt tatagcaagg cgagcgcggc
    24961 tggctgacct gaattaatgc ctctccccag cggaaaatat cgcccatatg aacattactt
    25021 tccgtgagac cgtcggttga gagattttcg ccaaacgcag gcgcaacaaa caactccgcc
    25081 tgttccggaa attcccgcac ccagtagaga tagtgctcac gaggataatg acacagcgct
    25141 ctgtctggcc cgccgtgaac ctttttctcc gcctgctcgt caccttccag ccccagctcc
    25201 gtcagcatca actcaccatc aacctggatt ttggcaattg cgctgggttt gccttcggga
    25261 taagcctgaa tcttgcctgt gtatacatca accggatatc gcatctgttc cctctccacg
    25321 agaaatttcc ccattagacc atatttattt tatgtcaggc gtgctgtttt cgcggtaagt
    25381 atccaggcag gaactcaata gcaaaccgga cgaacgttag ttcatccggc tgtggggatt
    25441 aaggaagcag gtttatttca ccgtacctaa aatttcgatt ttcgctgctc tggctttgac
    25501 tttacgtgac cagatagagg taagaattgg caccagaatc gaagtcacaa tgaccgccgt
    25561 cgctaccagc gaagttgctg ccggagccat cggtttaaac gcaggcacca tttctgcaat
    25621 cagcacaggt gtcgctaccg cggcccctgc ggaactggaa gcggcaattc cggcagtgcc
    25681 atcgccaccg ccaatcaatt tatcggcgat aatcaacgga ataccggtca cgataattac
    25741 tgccacaccc aacaggatcc ccagcaaacc agtctgggca atcacagtca aatcaatggt
    25801 attgcccagc gcgaaggcaa agaatggaat cagcgtttgc accgctttgc tgaaaaattc
    25861 tcgtaattca gggtcaaggt tccccagggc aaagcccacc aggaacggta atactgcgcc
    25921 gacgaaaaca tgcggttcaa acgaggcaat ccctgcagtg cccagaataa tcatcgtcat
    25981 gagcggcccg gactccaacg acatcaacac aaatgcccca gcttcttctt ttgtgccgta
    26041 ctgctgcatg atggaagcgt aaagtccgcc attggtcata tccatcgccg ccaccagcgc
    26101 cagcgttgaa agtccggcaa agaatccaac ttcaacacca tgttccggaa taatgcgcga
    26161 ggcaattgcc gcaaccaccc acgcgacggc aatttttgtt accaccagcg taccggattt
    26221 acgcaatacc gttcccgtcg cgcttaattt tattgacgcc cccatgcaaa aaaaccacac
    26281 cgccagaatg ggcaccgtac cggtaatcat cccgttggtg aatgatccaa aatatttccc
    26341 cgcccccggc gagaaggtgt ggcacagtgc gccaaggaat agcgggacga gcatcatccc
    26401 ccccgggatt ttctcaatcg agcgttttat ctgcatttcc atcacctgtc ggcatatttt
    26461 taagggctga tgcgtcattc ctcaatggag catcagtgtt gatggaaacg aagatagaca
    26521 ttcatttaat caataaaagt gatcaaaacc acatcaattg aaacgctgtt ttatttttat
    26581 cggatcattg tttttatttt gaaattgatc acaaaaaaac caccgccgtt ggcgatggtt
    26641 cattttttaa gctgatatgc ggcctatacg cctcattgca gcaggcggca aatgattatt
    26701 ttttcgccgc aaaacgtgcc gctgcttcgt cccagttcac cacgttccag aactctttaa
    26761 tgtagtccgg acggcggttc tggaatttca ggtagtaagc atgttcccac acatccaggc
    26821 ccagaatcgg gaagccggaa gcgccagaaa tagcttcacc catcagcgga gaatcctggt
    26881 tagcagtaga aaccaccgcc agtttatcgc ctttcagcac cagccatgcc cagccggaac
    26941 caaagcggga agctgccgct ttttcaaatt ctgctttgaa gttatcaacg gagccgaagt
    27001 cacgttcgat agccgctttc aggtcaccct gcagggtggt gccttttttc agacctttcc
    27061 agaacaggct gtggttagcg tgaccgccag cgttgttgcg cagtacggtt ttcttgtctg
    27121 ctggcagctg gtccagtttg gtgatcagct cttcaaccgg caggttggca aattctggca
    27181 ggctttccag cgccgcgttg gcgttgttta cgtaggtctg atggtgtttg gtgtggtgga
    27241 tttccatggt ctgcttatcg aagtgcggtt ccagggcatc gtaagcatac ggcagggatg
    27301 gcagggtata gctcatattc atctccagta ttgtcgggcg gccgattgtt aatgccgcgt
    27361 aagcagttgg ttcattatag ttaattaaat gatattgaaa atgattatca atgccgtact
    27421 tttcgtaagg gtatggtttt gcaggaaaat gcccgagatg tgaagcaaat cacccactta
    27481 atgccgtgat tgccagtaaa tcgacaacgg cggcaacagg cgaaaggtta atcgacagca
    27541 cgatttttac actcatctcg tcggagatgt gaagcgacga aaaatgatga ggataagaag
    27601 atgagtaacg cgattacgat ggggatattt tggcatttga tcggcgcggc cagtgcagcc
    27661 tgtttttacg ctccgttcaa aaaagtaaaa aaatggtcat gggaaaccat gtggtcagtc
    27721 ggtgggattg tttcgtggat tattctgccg tgggccatca gcgccctgtt actaccggat
    27781 ttctgggcgt attacagctc gtttagtctc tctacgctgc tgcctgtttt tctgttcggc
    27841 gctatgtggg ggatcggtaa tatcaactac ggcctgacca tgcgttatct cggcatgtcg
    27901 atgggaattg gcatcgccat tggcattacg ttgattgtcg gtacactgat gacgccaatt
    27961 atcaacggca atttcgatgt gctgattaac accgaaggcg gacgcatgac gttgctcggc
    28021 gttctggtgg cgctgattgg cgtagggatt gtaactcgag ccgggcagtt gaaagagcgc
    28081 aagatgggca ttaaagccga agagttcaat ctgaaaaaag ggctggtgct ggcggttatg
    28141 tgcggcattt tctctgccgg gatgtccttt gcgatgaacg ccgcaaaacc gatgcatgaa
    28201 gccgctgccg cacttggcgt cgatccactg tatgtcgctc tgccaagcta tgttgtcatc
    28261 atgggcggcg gcgcgatcat taacctcggt ttctgtttta ttcgtctggc aaaagtgaag
    28321 gatttgtcgc taaaagccga cttctcgctg gcaaaaccgc tgatcattca caatgtgtta
    28381 ctctcggcac tgggcgggtt gatgtggtat ctgcaattct ttttctatgc ctggggccac
    28441 gcccgcattc cggcgcagta tgactacatc agttggatgc tgcatatgag tttctatgta
    28501 ttgtgcggcg gtatcgtcgg actggtgctg aaagagtgga gcaatgcagg acgccgtccg
    28561 gtaacggtgt tgagcctcgg ttgtgtggtg attattgtcg ccgctaacat cgtcggcatc
    28621 ggcatggcga attaatcttt ctgcgaattg agatgacgcc actggctggg cgtcatcccg
    28681 gtttcccggg taaacaccac cgaaaaatag ttactatctt caaagccaca ttcggtcgaa
    28741 atatcactga ttaacaggcg gctatgctgg agaagatatt gcgcatgaca cactctgacc
    28801 tgtcgcagat attgattgat ggtcattcca gtctgctggc gaaattgctg acgcaaaacg
    28861 cgctcactgc acgatgcctc atcacaaaat ttatccagcg caaagggact tttcaggcta
    28921 gccgccagcc gggtaatcag cttatccagc aacgtttcgc tggatgttgg cggcaacgaa
    28981 tcactggtgt aacgatggcg attcagcaac atcgccaact gcccgaacag caactctgcc
    29041 atttcgttag caaacggcac atgctgacta ctttcatgct caagctgacc gataacctgc
    29101 cgcgcctgcg ccatccccac gctacctaag cgccagtgtg gttgccctgc gctggcgcta
    29161 aatcccggaa tcgccccctg ccagtcaaga ttcagcttca gccgctccgg gcaataaata
    29221 atattctgca aaaccagatc gttaacggaa gcgtaggagt gtttatcgtc agcatgaatg
    29281 taaaagagat cgccacgggt aatgcgataa gggcgatcgt tgagtacatg caggccatta
    29341 ccgcgccaga caatcaccag ctcacaaaaa tcatgtgtat gttcagcaaa gacatgttgc
    29401 ggataacggt cagccacagc gactgcctgc tggtcgctgg caaaaaaatc atctttgaga
    29461 agttttaact gatgcgccac cgtggctacc tcggccagag aacgaagttg attattcgca
    29521 atatggcgta caaatacgtt gagaagattc gcgttattgc agaaaaccat cccgtccctg
    29581 acgaatatca cgcggtgacc agttaaactc tcggcgaaaa agcgtcgaaa agtggttact
    29641 gtcgctgaat ccgcaatgat aggcgatgtc agtaacgctg gcctcactgt ggcgtagcag
    29701 atgtcgggcc ttcatcagtc gcaggcggtt caggtaacgc tgaggcgtca gtcccgtttg
    29761 ctgcttaagc tgccgatgta gcgttcgcag tgaaagagaa aattgatccg ccacggcatc
    29821 ccaattcacc tcatcggcaa aatggtcctc cagccaggcc agaagcaagt tgagacgtga
    29881 tgcgctgttt tccaggttct tctgcaaact gcttttacgc agcaagagca gtaattgcat
    29941 aaacaaagat ctcgcgactg gcggtcgagg gtaaatcatt ttccccttcc tgctgttcca
    30001 tctgtgcaac cagctgtcgc acctgctgca atacgctgtg gttaacgcgc cagtgagacg
    30061 gatactgccc atccagctct tgtggcagca actgattcag cccggcgaga aactgaaatc
    30121 gatccggcga gcgatacagc acattggtca gacacagatt atcggtatgt tcatacagat
    30181 gccgatcatg atcgcgtacg aaacagaccg tgccaccggt gatggtatag ggctgcccat
    30241 taaacacatg aatacccgtg ccatgttcga caatcacaat ttcatgaaaa tcatgatgat
    30301 gttcaggaaa atccgcctga gggagccggg gttctatcgc cacggacgcg ttaccagacg
    30361 gaaaaaaatc cacactatgt aatacggtca tactggcctc ctgatgtcgt caacacggcg
    30421 aaatagtaat cacgaggtca ggttcttacc ttaaattttc gacggaaaac cacgtaaaaa
    30481 acgtcgattt ttcaagatac agcgtgaatt ttcaggaaat gcggtgagca tcacatcacc
    30541 acaatgaagc aaattgtgaa catcatcacg ttcatctttc cctggttgcc aatggcccat
    30601 tttcctgtca gtaacgagaa ggtcacgaat tcaggcgctt tttagactgg tcgtaatgaa
    30661 attcagcagg atcacattat gacctttcgc aattgtgtcg ccgtcaatct cggcgcatcc
    30721 agtgggcgcg tgatgctggc gcgttacgag cgtgaatgcc gcagcctgac gctgcgcgaa
    30781 atccatcgtt ttaaaaatgg gctgcatagc cagaacggtt atgtcacctg ggatgtggat
    30841 agcctggaaa gtgccattcg ccttggctta aacaaagtgt gtgaggaagg gattcgtatc
    30901 gatagcattg ggattgatac ctggggcgtg gactttgtgc tgctcgacca acagggtcag
    30961 cgtgtgggcc tgcccgttgc ttatcgcgat agccgcacca atggcctaat ggcgcaggca
    31021 cagcaacaac tcggcaaacg cgatatttat caacgtagcg gcatccagtt tctgcccttc
    31081 aatacgcttt atcagttgcg tgcgctgacg gagcaacaac ctgaacttat tccacacatt
    31141 gctcacgctc tgctgatgcc ggattacttc agttatcgcc tgaccggcaa gatgaactgg
    31201 gagtacacca atgccaccac cacacaactg gtcaatatca atagcgacga ctgggacgag
    31261 tcgctactgg cgtggagcgg ggccaacaaa gcctggtttg gtcgcccgac gcatccgggt
    31321 aatgtcattg ggcactggat ttgcccgcag ggtaatgaga ttccggtggt cgccgttgcc
    31381 agccatgata ccgccagcgc ggttatcgcc tcgccgttaa acggttcacg cgccgcttat
    31441 ctctcttctg gcacctggtc attgatgggc ttcgaaagcc agacgccatt taccaatgac
    31501 acggcgctgg cagccaacat caccaatgaa ggcggggcgg aaggtcgcta tcgggtgctg
    31561 aaaaatatta tgggcttatg gctgcttcag cgagtgcttc aggaacggca aatcaacgat
    31621 ctcccggcgc ttatcgccgc gacacaggca cttccggcct gccgcttcat catcaatccc
    31681 aatgacgatc gctttattaa tcctgaggcg atgtgcagcg aaattcaggc tgcgtgtcgg
    31741 gaaacggcgc aaccgatccc ggaaagtgat gctgaactgg cgcgctgtat tttcgacagt
    31801 ctggcgctgc tgtatgccga tgtgttgcat gagctggcgc agctgcgcgg tgaagatttc
    31861 tcgcaactgc atattgtcgg cggcggctgc cagaacacgc tgctcaacca gctatgcgcc
    31921 gatgcctgcg gtattcgggt gatcgccggg cctgttgaag cctcaacgct cggcaatatc
    31981 ggcatccagt taatgacgct ggatgaactc aacaatgtgg ataatttccg tcaggtcgtc
    32041 agcaccaccg cgaatctgac cacctttacc cctaatcctg acagtgaaat tgcccactat
    32101 gtggcgcaga ttcactctac acgacagaca aaggagcttt gcgcatgacc actcaactgg
    32161 aacaggcctg ggagctggcg aaacagcgtt tcgcggcggt ggggattgat gtcgaggagg
    32221 cgctgcgcca acttgatcgt ttacccgttt caatgcactg ctggcagggc gatgatgttt
    32281 ccggttttga aaacccggaa ggttcgctga ccggtgggat tcaagctact ggtaattatc
    32341 cgggcaaagc gcgtaatgcc agtgagctac gtaccgatct ggaacaggct atgcggctga
    32401 ttccggggcc gaaacggctt aatttacatg ccatctatct ggaatcagac acgccagtct
    32461 cgcgcgacca gatcaaacct gagcacttca aaaactgggt tgaatgggcg aaagccaatc
    32521 agctcggtct ggattttaac ccctcctgtt tttcgcatcc gctaagcgcc gatggcttta
    32581 cgctttccca tgccgacgac agcattcgcc agttctggat tgatcactgc aaagccagcc
    32641 gtcgcgtttc ggcctatttt ggcgagcaac tcggcacacc atcggtgatg aacatctgga
    32701 tcccggatgg tatgaaagat atcaccgttg accgtctcgc cccgcgtcag cgtctgctgg
    32761 cagcactgga tgaggtgatc agcgagaagc taaaccctgc gcaccatatc gacgccgttg
    32821 agagcaaatt gtttggcatt ggcgcggaga gctacacggt tggctccaat gagttttacc
    32881 tggggtatgc caccagccgc cagacggcgc tgtgcctgga cgccgggcac ttccacccga
    32941 ctgaagtgat ttccgacaag atttccgccg ccatgctgta tgtgccgcag ttgctgctgc
    33001 acgtcagtcg tccggttcgc tgggacagcg atcacgtagt gctgctggat gatgaaaccc
    33061 aggcaattgc cagtgagatt gtgcgtcacg atctgtttga ccgggtgcat atcggccttg
    33121 acttcttcga tgcctctatc aaccgcattg ccgcgtgggt cattggtaca cgcaatatga
    33181 aaaaagccct gctgcgtgcg ttgctggaac ctaccgccga gctgcgcaag ttggaagcgg
    33241 cgggcgatta cactgcgcgt caggcactgc tggaagagca gaaatcgttg ccgtggcagg
    33301 cggtatggga aatgtattgt cagcgtcacg ataccccagc gggtagcgaa tggctggaga
    33361 gcgtgcgggc ttatgagaaa gaaattttga gtcagcgtgg gtaaacactg ccagatgcgg
    33421 cacgagcgcc ttatccggcc tacagatcgt gcatctgaca acaccccatt tacaggaaca
    33481 ccgaacatgc aaaacattac tcagtcctgg tttgtccagg gaatgatcaa agccaccacc
    33541 gacgcctggc tgaaaggctg ggacgagcgc aacggcggca acctgacgct acgcctggat
    33601 gacgctgaca tcgcaccgta tcacgacaat ttccaccaac aaccgcgtta tatcccgctc
    33661 agccaaccca tgcctttact ggcaaataca ccgtttatcg tcaccggctc gggcaaattc
    33721 ttccgtaacg tccagcttga tcctgcggct aacttaggcg tcgtaaaagt cgacagcgac
    33781 ggcgcgggct accatattct ctgggggtta accaacgagg ccgttccaac ttctgaactt
    33841 ccggcacact ttctctccca ttgcgaacgc attaaagcca ccaacggtaa agatcgggtg
    33901 atcatgcact gccatgccac caacctgatc gccctcacct atgtactgga aaacgacacg
    33961 gcggtcttca ctcgccaact gtgggaaggc agcaccgagt gcctggtggt attcccggat
    34021 ggcgttggca ttctgccgtg gatggtgccc ggcacggacg aaatcggtca ggcgaccgca
    34081 caagaaatgc aaaaacattc gctggtgttg tggcccttcc acggtgtgtt cggttccgga
    34141 ccgacactgg atgaagcctt tggtttgata gataccgcag agaaatcagc ggaggtgtta
    34201 gtcaaaatct actcgatggg cggtatgaag cagaccatta cccgtgagga gctgattgca
    34261 ctcggtgagc gttttggcgt tacgccactg gccagtgcgc tggcacttta atatcccaac
    34321 agatatccgg cggtgtggga aacgtcgccg gacagatgga tttgaactta aggagagtca
    34381 ttatgagttt tatgttggca ttaccgaaaa ttagcctgca tggtgctggg gcaattggcg
    34441 atatggtaaa tctggttgcg cataaacagt ggggaaaagc actgatcgtt accgatgggc
    34501 aactggttaa gttgggatta ctggacagcc tgtttatcgc actggatcaa tacgaaatgt
    34561 cttatcactt atttgatgag gtatttccta accccactga agaactggtg caaaaaggct
    34621 acgctgcatt tcaggatgcc gcatgtgact acattattgc tttcggtggc ggtagcccaa
    34681 ttgatactgc aaaagccgta aaaatcctta ccgctaatcc tgcgccttct acggcgtact
    34741 ctggtgttgg taaagtgaca aatcccggcg taccgctggt ggcaatcaat acgactgccg
    34801 gaacagctgc tgagatgacc agcaatgcgg tgatcatcga caccgcgcgg caggtaaaag
    34861 aggtcattat cgacccgaat attattcccg atattgcggt ggatgatgcc agtgtaatgc
    34921 tggagatccc ggcctcggtt accgccgcca cgggtatgga tgcactgact cacgcagttg
    34981 aagcttatgt ttctgttggt gcacatccat taaccgacgc taacgcactg gaagctatcc
    35041 gtttaattac gctgtggctg ccaaaagcgg tagatgatgg tcataatctg gaagctcgtg
    35101 aacaaatggc attcgcccag tacctggcgg gtatggcatt taacagtgct ggtctgggcc
    35161 ttgttcatgc acttgcgcat cagcccggcg ccacccacaa cctgccgcat ggtgtgtgca
    35221 atgctatctt gttgccagtt attgaaaact ttaaccgccc gaatgccatc gcacgctttg
    35281 cccgcattgc tcaggcaatg ggtgtcgata cgcgtgatat gagtgaagaa gcggcaagta
    35341 ctgaagcaat taacgctatc cgcgcattaa gccatcgcgt gggtattccg gcaggattca
    35401 gccagcttgg catcaccaaa gaagatattg aaggctggtt agataaagcc ctggctgacc
    35461 cgtgtgtgcc atgtaacccg cgtaccgcca gccgcgacga ggtgcgcgag ctgtatctgg
    35521 aggcattatg atccgtaagg catttgtgat gcaggttaac cccgacgctc acgaagagta
    35581 tcaacgtcgg cataacccca tctggccgga actggaagca gtgttgaaat ctcacggtgc
    35641 gcataactac gccatttacc tcgacaaagc gcgtaatctg ctgtttgcca cggtagagat
    35701 tgaatctgaa gagcgctgga atgcggttgc cagtactgat gtttgccaac gttggtggaa
    35761 atatatgacc gacgtcatgc ccgcgaaccc ggataacagc ccggtgagta gcgaactgca
    35821 agaagtgttt tacctgccgt aattgcctaa tgtgccggag acgcctccgg tacaatattt
    35881 ttaatttcga tttgaaaaat aaacatatta caccttcagt taatatatct cggtaactta
    35941 tatttccccg atgcattgtg agtaaatcac aaaaataatg aataactcat taatgattca
    36001 tgtggtttat ttaaataacc attatgtgca ttactccgca aatctggcct ttcactctgt
    36061 ctcaaattgt cattgtttac cgacatatca cctctcatca cttaatcgct ccaccattac
    36121 aattgattaa aatatatttt atggagtccg attaatggca gctcttactg caagctgtat
    36181 tgacctgaat attcagggca atggcgctta ttccattctg aagcagttgg cgacaatggc
    36241 gttacaaaac ggttttatca ccgactcaca ccagttcctg caaaccctgc tgctgcgcga
    36301 aaaaatgcac tctactggat ttggttccgg catcgccgtg ccgcacggta aaagcgcctg
    36361 cgttaaacaa ccgttcgtgt tattcgcccg caaagcgcag gccattgact ggaaagccag
    36421 cgacggcgaa gacgtcaatt gctggatctg cctcggcgtg ccgcaaagtg gcgaagagga
    36481 tcaggtcaaa atcatcggca cactgtgtcg caaaattatc catcaggatt ttatcaacca
    36541 actgcgacag ggcgacgccg accaggtact tgctttgtta aatcaaaccc tcagctcata
    36601 aggaagtggc gatggagtca tccttacgta ttgtcgcgat caccaactgc cccgccggga
    36661 tcgctcacac ctacatggtg gcggaagccc tggaacagaa agcccgttct ctcggccata
    36721 ccataaaagt ggaaactcaa gggtccagcg gcgttgaaaa ccgcttatcc agcgaagaga
    36781 ttgccgctgc cgattacgtc attctagcta ccgggcgtgg cctgagcggt gatgaccggg
    36841 cgcggtttgc cgggaagaaa gtttatgaga ttgccatctc ccaggcgctg aaaaatatcg
    36901 accagatttt cagcgaatta ccaacaaact cgcagctttt tgccgcagat agcggcgtga
    36961 agctcggcaa acaggaagtg caaagcggca gcgtgatgag ccacctgatg gcgggcgtct
    37021 ctgcggcgct gccatttgtc atcggcggcg gtattctggt ggcgcttgcc aacatgctgg
    37081 tgcagttcgg tttgccatat accgatatgt cgaaaggtgc gccgtcgttt acctgggtag
    37141 ttgaatccat cggttacctc ggcttcacct ttatgatccc catcatgggc gcttatattg
    37201 cctcgtcgat tgccgataaa cccgctttcg ctccggcatt tctggtttgc tatctggcga
    37261 acgataaagc cctgctcggc acccagtcag gcgcgggctt cctcggcgcg gtggtgctgg
    37321 ggctggcgat tggctatttc gtcttctggt tccgtaaagt ccgtcttggc aaagcattac
    37381 aaccgctgct cggctccatg cttatcccat tcgttactct gctggttttt ggcgtgctca
    37441 cttactacgt tatcgggccg gtgatgtccg acctcatggg cgggctgctc cacttcctga
    37501 ataccattcc tccatcaatg aagtttgcgg cagcgtttct ggtcggtgcg atgctggcgt
    37561 tcgatatggg cggccccatc aataaaaccg cctggttctt ctgcttctca ctgctggaaa
    37621 aacacattta tgactggtac gccatcgtcg gcgttgtcgc gctgatgccg cccgtcgcgg
    37681 cgggtcttgc gacgttcatt gcgcccaaac tgtttacccg acaggaaaaa gaggctgcca
    37741 gtagtgccat tgtggtcggt gccacagtcg caaccgaacc ggctattcct tacgctctgg
    37801 cagctccgtt gccgatgatt accgccaata cgctggcggg cggtattacc ggcgtgctgg
    37861 tgatcgcttt cggaatcaaa cgtctggcac ctggtttggg tatctttgac ccgctgatcg
    37921 gcctgatgtc accggtaggt tcgttttatc tggtgctggc gattggcctc gcgctgaaca
    37981 tttcattcat tatcgtcctg aaaggattgt ggttacgccg caaagcgaaa gccgcgcaac
    38041 aggagcttgt ccatgaacat tgagttactg caacagttgt gcgaagccag cgccgttagc
    38101 ggcgatgaac aggaagttcg cgacattctg ataaacactc tggaaccttg cgtgaatgaa
    38161 atcacctttg atgggctggg cagctttgtc gcccgtaagg gaaataaagg gccgaaagtt
    38221 gccgttgtcg ggcatatgga tgaagtcggc tttatggtca cccacatcga cgagagcggt
    38281 tttctgcgtt tcaccaccat tggtggctgg tggaatcagt cgatgctcaa ccaccgggta
    38341 accatacgca cacacaaggg agtgaaaatc cctggtgtga ttggttccgt cgcgcctcat
    38401 gcgttaacgg aaaagcaaaa gcaacagccg cagtcatttg atgagatgtt cattgatatt
    38461 ggcgcaaaca gtcgcgatga ggtggaaaaa cgcggtgtgg cgatgggcga ttttattagc
    38521 ccggaagcca attttgcctg ctggggcgaa gataaagtgg tcggcaaggc gttggataac
    38581 cgcatcggct gcgcaatgat ggctgagcta ctacagacgg tgaataatcc cgaaattacg
    38641 ctgtatggcg ttggcagtgt ggaagaagaa gttgggctac gcggggcgca aacctcggct
    38701 aaacacatta aaccggatgt ggtgatcgtg ctggacaccg ctgtcgcggg cgatgttcca
    38761 ggcattgata acattaaata tccgctgaaa ctgggcaacg ggccggggtt gatgctgttt
    38821 gacaagcgct acttccccaa ccagaaactg gtagcagcgt taaaaagctg tgccacgcag
    38881 aatgatttac cgctgcaatt ttccaccatg aaaaccggtg cgacggatgg cgggcgctac
    38941 aacgtgatgg gcggtgggcg tccggttgtc gcgctgtgtt tgccaacgcg ttatctgcac
    39001 gccaacagcg gcatgatttc aaaagccgat tacgatgctc tgctcacgct gatacggggt
    39061 tttctgacca ccttaaccgc ggaaaaagtc agcgcgttta gccagttccg tcaggtggat
    39121 taatgctaaa cgaacgccag ttaaagattg tcgatctgct ggagcaacag ccgcgcacgc
    39181 ctggcgagct ggcgcaacag actggcgttt ctggcaggac catactgcgt gatattgact
    39241 atctcaactt tacccttaac ggcaaagccc gcatttctgc cagtggtagt gcgggctatc
    39301 agctggaaat cttcgagcgc cgcagctttt ttcagttgct gcaaaagcac gataacgacg
    39361 atcggctgct ggcactgtta ttactgaata cctttactcc ccgtgcgcaa ctcgcctcgg
    39421 cgcttaattt gccggaaacg tgggtcgcag agcgtctgcc ccggttaaaa cagcgttatg
    39481 aacgcacttg ctgcctggcc agtcgccccg gtttgggcca tttcattgat gagacagaag
    39541 aaaaacgcgt tatattgctg gcgaacttgc tgcgcaaaga tccgttttta attccgctgg
    39601 cgggcgtaac acgagacaac cttcagcatt tatccacggc ctgcgacaac caacaccgct
    39661 ggccgctcat gcagggtgat tatctctcca gcctgattct ggcgatttac gccctgcgta
    39721 atcaactgac cgatgagtgg ccgcaatatc ccggtgacga gataaaacaa atcgttgaac
    39781 atagcggtct gtttcttggt gataacgctg taagaaccct gacaggtttg atagagaaac
    39841 agcatcagca agcgcagata atttctgccg ataatgtgca gcggttgctg caaagggtgc
    39901 cgggcatcgc gtcattgaat attattgata cgcagctggt tgagaacatt accgggcatt
    39961 tattacgttg ccttgccgca ccggtgtgga ttgctgagca ccgccaaagc agcatgaata
    40021 acctgaaagc cgcctggcct gcggcctttg atatgagtct gcactttatt acgctactgc
    40081 gtgaacagct cgatattccc cttttcgaca gcgatctgat cggtttgtat tttgcctgtg
    40141 cgctggaacg gcatcaaaac gaacgccagc cgatcatttt gctctcggac cagaacgcga
    40201 ttgccactat taatcagctc gccattgagc gcgatgtttt acattgtcgg gtgattattg
    40261 cccgtagctt aagcgaactt gttgccattc gcgaagagat tgagccgtta ttgatcatta
    40321 acaacagcca ttatttactg gatgacgcgg taaataatta catcaccgta aaaaatatca
    40381 ttacggctgc cggtatcgaa caaataaaac attttctggc gacggcattt attcgccaac
    40441 agccggagcg ttttttctct gctcccggaa gttttcatta ttcgaatgta cgcggtgaaa
    40501 gctggcaaca tattacccgg caaatttgtg cgcaattagt cgcacaacac catattaccg
    40561 ccgatgaagc acaacgcatc atcgcccgcg aaggcgaagg tgaaaacctg attgttaatc
    40621 gcctcgccat cccacattgc tggagcgaac aggagcgacg ttttcgtgga ttttttatta
    40681 ccctcgccca accagttgag gtgaataacg aagtcattaa ccatgtcttg atcgcctgcg
    40741 ccgccgccga tgcgcgtcat gagctaaaaa tatttagcta tctggcaagc atattgtgtc
    40801 agcatccggc ggagattatt gccgggttaa caggatatga ggcatttatg gagttacttc
    40861 acaaggggtg aactgtgtcg gatgcgccgc atccgacact gcatttatca tcaaaaacgt
    40921 ttaacgcagg ctattatagt catcaaccat ttcgttgtac tcgcgtaatg ctctcgggaa
    40981 agagtcttcg accatccagc ttgagtttgc gtcctgaagt tgctctttat cccagtcaga
    41041 gtacgggact ttatctctga tgcggcgaac gtatttttta gcctcgagtt gatactggcc
    41101 tgccgaatta ataaacgaga aattcatgcc gcctttatcc gcttctttgg cctgcgccac
    41161 tagcgtttcc agttcagaca ctttcttcat tgccgcatca acatcaaact tctcttgcga
    41221 tatcaggtta ttaatttgtt tcgctgaaat catgactgcc agagagtagt agtggaatgt
    41281 tttcccttct ctttcttcaa tatttttcag ttccgcaagc tggcgcttat cgttaatttc
    41341 ctgaatcgcc gcatgataag attctgctac aggttcaaag gcttccagat tttttaagaa
    41401 tgtctggtgc agcgttttcc ctttcgcaaa cgcatcgtct ttataatttt cctgggtgta
    41461 atatttatcc atttcgttaa tggtattacc caacgccacg gcagcatcga tataactcac
    41521 cgccacgcca tcaattggtt gcagcgcagg cgttaacgcc acagcacttt ttacgccttt
    41581 ttcacactca gcgaggttgg attcactaat gccgtagatg ccataaacag tacgctcttc
    41641 accggtaggc ccctgtttaa aatctttcag ccagtcagca taacgcgcca gactgctctg
    41701 tacagggatc tgtaacttgt tatagcactt gatatagaca tccatttttt cgtcgaaaac
    41761 cgcttgttca tcgaccaccg gttgcgcagg tgtttcaggt ttagcttctg cttttgcaac
    41821 gggagcttca ggagctggtg cggcaggttg actattggca ggcgggggcg tttctgtttc
    41881 ggctttttta tcatcacaac ccgtcagacc gagggccatg atggcgacta taatagcgga
    41941 agataataaa ttcctt
//
LOCUS       DAZGJW010000024        41500 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZGJW010000024 DAZGJW010000000
VERSION     DAZGJW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.024"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            234..1331
                     /gene="mltA"
                     /locus_tag="ACHKIK_001354"
     CDS             234..1331
                     /gene="mltA"
                     /locus_tag="ACHKIK_001354"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO9082561.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1408..2214
                     /gene="tcdA"
                     /locus_tag="ACHKIK_001355"
     CDS             1408..2214
                     /gene="tcdA"
                     /locus_tag="ACHKIK_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO9082562.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2265..2708)
                     /gene="csdE"
                     /locus_tag="ACHKIK_001356"
     CDS             complement(2265..2708)
                     /gene="csdE"
                     /locus_tag="ACHKIK_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO9082563.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAIEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2708..3913)
                     /gene="csdA"
                     /locus_tag="ACHKIK_001357"
     CDS             complement(2708..3913)
                     /gene="csdA"
                     /locus_tag="ACHKIK_001357"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO9082564.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQLGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLNIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4105..4332
                     /gene="ygdI"
                     /locus_tag="ACHKIK_001358"
     CDS             4105..4332
                     /gene="ygdI"
                     /locus_tag="ACHKIK_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO9082565.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4683..5600
                     /gene="gcvA"
                     /locus_tag="ACHKIK_001359"
     CDS             4683..5600
                     /gene="gcvA"
                     /locus_tag="ACHKIK_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO9082566.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5619..6014
                     /gene="ygdD"
                     /locus_tag="ACHKIK_001360"
     CDS             5619..6014
                     /gene="ygdD"
                     /locus_tag="ACHKIK_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO9082567.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6007..7107
                     /gene="rlmM"
                     /locus_tag="ACHKIK_001361"
     CDS             6007..7107
                     /gene="rlmM"
                     /locus_tag="ACHKIK_001361"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO9082568.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7151..7882)
                     /gene="fucR"
                     /locus_tag="ACHKIK_001362"
     CDS             complement(7151..7882)
                     /gene="fucR"
                     /locus_tag="ACHKIK_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO9082569.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7940..8362)
                     /gene="fucU"
                     /locus_tag="ACHKIK_001363"
     CDS             complement(7940..8362)
                     /gene="fucU"
                     /locus_tag="ACHKIK_001363"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO9082570.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8364..9782)
                     /gene="fucK"
                     /locus_tag="ACHKIK_001364"
     CDS             complement(8364..9782)
                     /gene="fucK"
                     /locus_tag="ACHKIK_001364"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO9082571.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9891..11666)
                     /gene="fucI"
                     /locus_tag="ACHKIK_001365"
     CDS             complement(9891..11666)
                     /gene="fucI"
                     /locus_tag="ACHKIK_001365"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO9082572.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQRRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11699..13015)
                     /gene="fucP"
                     /locus_tag="ACHKIK_001366"
     CDS             complement(11699..13015)
                     /gene="fucP"
                     /locus_tag="ACHKIK_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO9082573.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13562..14209
                     /gene="fucA"
                     /locus_tag="ACHKIK_001367"
     CDS             13562..14209
                     /gene="fucA"
                     /locus_tag="ACHKIK_001367"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO9082574.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14237..15385
                     /gene="fucO"
                     /locus_tag="ACHKIK_001368"
     CDS             14237..15385
                     /gene="fucO"
                     /locus_tag="ACHKIK_001368"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO9082575.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15440..16195)
                     /gene="xni"
                     /locus_tag="ACHKIK_001369"
     CDS             complement(15440..16195)
                     /gene="xni"
                     /locus_tag="ACHKIK_001369"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO9082576.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16307..17674)
                     /gene="sdaB"
                     /locus_tag="ACHKIK_001370"
     CDS             complement(16307..17674)
                     /gene="sdaB"
                     /locus_tag="ACHKIK_001370"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO9082577.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17732..19021)
                     /gene="sdaC"
                     /locus_tag="ACHKIK_001371"
     CDS             complement(17732..19021)
                     /gene="sdaC"
                     /locus_tag="ACHKIK_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO9082578.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(19578..20942)
                     /gene="ppnN"
                     /locus_tag="ACHKIK_001372"
     CDS             complement(19578..20942)
                     /gene="ppnN"
                     /locus_tag="ACHKIK_001372"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO9082579.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(21054..21902)
                     /gene="queF"
                     /locus_tag="ACHKIK_001373"
     CDS             complement(21054..21902)
                     /gene="queF"
                     /locus_tag="ACHKIK_001373"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO9082580.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDANLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            21970..22515
                     /gene="syd"
                     /locus_tag="ACHKIK_001374"
     CDS             21970..22515
                     /gene="syd"
                     /locus_tag="ACHKIK_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO9082581.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23137..23466
                     /gene="yqcC"
                     /locus_tag="ACHKIK_001375"
     CDS             23137..23466
                     /gene="yqcC"
                     /locus_tag="ACHKIK_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO9082582.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            23466..24248
                     /gene="truC"
                     /locus_tag="ACHKIK_001376"
     CDS             23466..24248
                     /gene="truC"
                     /locus_tag="ACHKIK_001376"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO9082583.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            24266..24715
                     /gene="yqcA"
                     /locus_tag="ACHKIK_001377"
     CDS             24266..24715
                     /gene="yqcA"
                     /locus_tag="ACHKIK_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO9082584.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            25150..26502
                     /gene="gudP"
                     /locus_tag="ACHKIK_001378"
     CDS             25150..26502
                     /gene="gudP"
                     /locus_tag="ACHKIK_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO9082585.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            26504..27844
                     /gene="gudX"
                     /locus_tag="ACHKIK_001379"
     CDS             26504..27844
                     /gene="gudX"
                     /locus_tag="ACHKIK_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO9082586.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            27865..29205
                     /gene="gudD"
                     /locus_tag="ACHKIK_001380"
     CDS             27865..29205
                     /gene="gudD"
                     /locus_tag="ACHKIK_001380"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO9082587.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGKYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(29437..32193)
                     /gene="barA"
                     /locus_tag="ACHKIK_001381"
     CDS             complement(29437..32193)
                     /gene="barA"
                     /locus_tag="ACHKIK_001381"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO9082588.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            32250..33551
                     /gene="rlmD"
                     /locus_tag="ACHKIK_001382"
     CDS             32250..33551
                     /gene="rlmD"
                     /locus_tag="ACHKIK_001382"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO9082589.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            33599..35833
                     /gene="relA"
                     /locus_tag="ACHKIK_001383"
     CDS             33599..35833
                     /gene="relA"
                     /locus_tag="ACHKIK_001383"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO9082590.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            35911..36159
                     /gene="mazE"
                     /locus_tag="ACHKIK_001384"
     CDS             35911..36159
                     /gene="mazE"
                     /locus_tag="ACHKIK_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO9082591.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            36159..36494
                     /gene="mazF"
                     /locus_tag="ACHKIK_001385"
     CDS             36159..36494
                     /gene="mazF"
                     /locus_tag="ACHKIK_001385"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO9082592.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            36565..37356
                     /gene="mazG"
                     /locus_tag="ACHKIK_001386"
     CDS             36565..37356
                     /gene="mazG"
                     /locus_tag="ACHKIK_001386"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO9082593.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            37584..39221
                     /gene="pyrG"
                     /locus_tag="ACHKIK_001387"
     CDS             37584..39221
                     /gene="pyrG"
                     /locus_tag="ACHKIK_001387"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO9082594.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            39309..40607
                     /gene="eno"
                     /locus_tag="ACHKIK_001388"
     CDS             39309..40607
                     /gene="eno"
                     /locus_tag="ACHKIK_001388"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO9082595.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(40667..>41500)
                     /gene="ygcG"
                     /locus_tag="ACHKIK_001389"
     CDS             complement(40667..>41500)
                     /gene="ygcG"
                     /locus_tag="ACHKIK_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO9082596.1"
                     /translation="FVAAQPTIVPQLQQQVTDLTSSLNSQEKKELTHKLESIFNNTQV
                     QIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSDRTVRIQVGYGLEE
                     KVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQYPTNPSESESASSS
                     DHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVYISAIYLLDLFLFSDKIFSIAVF
                     SFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSGGGGSSGGGGASGR
                     W"
BASE COUNT        10298 a        10044 c        11017 g        10141 t
ORIGIN      
        1 agctaccagg ctgctccacc ccgcgtccgt ggatgcgcac tatactccgc tcgcgttttc
       61 atgcaacctt ttttttaccc cgatcacgga tttagcatga ttttgaacaa aaacaaacca
      121 ctaaagccta ttttttgtgc aaaatttgcg tcagggagag gctgctcatt tcattaaacg
      181 cggcggtttg ttatcttcgt tgcgccttat tttttaacct gaagaagaga acaatgaaag
      241 gacgttgggt aaagtacctt cttatgggca cggttgtggc aatgcttgcc gcctgctctt
      301 ccaaaccaac cgatcgcgga cagcaatata aagacgggaa atttacccag cctttctctc
      361 tggtgaacca gccagatgcc gttggcgcgc cgattaacgc cggtgatttt gccgagcaaa
      421 ttaaccatat ccgtaattcg tcaccgcgtc tgtatggcaa ccagagtaat gtttataacg
      481 cggtgcaaga gtggctgcgc gcaggcggtg atacccgcaa tatgcgccag ttcggcattg
      541 atgcctggca gatggaaggt gccgacaact atggtaacgt gcagtttaca ggctattaca
      601 cgccggtaat tcaggcgcgc catacccgcc agggcgagtt ccagtatcct atttaccgta
      661 tgccgccaaa acgtggtcgt ctgccgtctc gtgcggagat ctacgcaggg gcgctgagtg
      721 ataaatatat tctcgcttac agcaactccc tgatggataa cttcattatg gatgtgcagg
      781 gtagcgggta tatcgacttt ggtgatggca gtccgcttaa ctttttcagc tatgcaggga
      841 aaaacggtca tgcctatcgc agcattggta aggtgctgat cgaccgtggc gaagtgaaaa
      901 aagaagatat gtcgatgcag gcgattcgtc attggggcga aacacacagt gaagccgagg
      961 ttcgcgagct gctggaacag aacccgtctt tcgttttctt taaaccgcaa tcttttgcac
     1021 cggtgaaagg ggcaagtgcg gtgccgctgg ttggccgtgc ttctgttgcc tccgatcgtt
     1081 caattattcc gccaggtact accttgctgg cagaagtgcc gctgctggat aataacggca
     1141 aatttaacgg tcagtacgaa ttgcgtctga tggtggcgct ggatgtcggt ggcgcaatca
     1201 aaggccaaca cttcgatatc tatcaaggga tcgggccgga agccggacat cgcgcaggtt
     1261 ggtacaacca ctatggacgt gtctgggtgc tgaaaaccgc cccgggcgca ggtaacgtct
     1321 ttagcggctg atgtgttatt ctgtataccg tttttacgga tacgacaggg tgagggataa
     1381 cctcaccctt tttaattctg aggtgctatg tctgtggtaa ttagtgatgc atggcgtcag
     1441 cgttttggtg gcacagcgcg tctgtatggt gaaaaagcgt tgcaactgtt tgctgacgcg
     1501 catatttgtg tggttggtat cggtggtgtc ggttcctggg cggcggaagc gctggcgcgc
     1561 acggggattg gcgcaatcac gcttatcgat atggatgatg tgtgcgtcac caataccaat
     1621 cggcaaattc acgctctgcg cgataacgtc gggctggcaa aagcggaagt catggcggag
     1681 cgtattcgcc agattaaccc ggagtgccgt gtaacggtgg tggatgattt cgtgacgccg
     1741 gataacgtag cgcagtatat gagcgtgggg tattcgtacg tgattgatgc cattgatagt
     1801 gtacggccca aagcggcgct gattgcttat tgtcggcgca ataaaatccc gctggtcacg
     1861 accggtggcg cgggtgggca gattgacccg acgcagattc aggttaccga tctggcgaaa
     1921 acgattcagg atccgctggc ggcgaagttg cgcgagcgcc tgaaaagcga ttttggcgta
     1981 gtgaaaaaca gtaaaggtaa gctcggcgtc gattgcgtgt tttctactga agcgctggtg
     2041 tacccgcagt cagacggtac tgtgtgtgcg atgaaagcca cggcagaagg gccgaagcgg
     2101 atggattgtg catctggatt tggcgcggca acgatggtga ccgccacctt tgggtttgtt
     2161 gcggtttctc atgcgctgaa gaagatgatg gcgaaagcgg cgcgtcaggg ttaaacaatc
     2221 aggtcggatg cgacgcgaac gtcttatccg acctggttct gtgcttaaac ctgcttcgtc
     2281 gcagcgataa tcgcctcgct taacgcattc aacccctggc tgcgtgaggc gctaagctgc
     2341 ccacgcaatc ccagctcatc aaacaatgcc agcggcgact gtgcctgcaa ttcggcggcg
     2401 gttttcccct caatggcagt caacagcacc gccagcaggc cgcgcactat acgtccttcg
     2461 ctgtcgccaa agaagtgcat tttaccgttt tcagccacgg tatatcccag ccagacgcgg
     2521 ttttcgcatc cggcaatctc tttagcctgc gcttttaact cgtctggcaa agccggaagc
     2581 tgtttcccca gcatgatcaa ctggcgatat ttatcttccc attgcgtcaa cggtgcgaag
     2641 gtattgcgta acgtttctgc ggttacggtt gtgccgaacg gatgtccggc gaattgcggg
     2701 ttcgtcatta atccaccaat aattccagcg cgcggtcaac ggcattcacc agcgcatcca
     2761 catcactctt tgtattatat ggcgcaaaag aggcgcgcag tgtgccggtt acgcctaatt
     2821 ctgccagtag cggctgagcg caatgttgcc cggcccgcaa ggcaataccg tactccgcca
     2881 gcagcgtcac catatcgctg tgatgaacgc cagcaaaatc aaaggccagc aggctggaat
     2941 cctggcagcg gaatgaacgg aaacctggac gtttcgccag cgcatcttcc gccagtgttg
     3001 ctaagctacg gctccagttt tcggcctggt tgatatcgta atctgccagc cattccagcg
     3061 ccgcgcttaa tcctatgaca ccagccacat ttggcgttcc tgcttccagt ttccacggcg
     3121 cagattgagt cgtgaagccg tcaaaactca cttcgtgaac cattttgccg ccgcccagcc
     3181 agggcgacat cgcttccagc agttctgatt tgccatacag cacgccgata cctgtcgggc
     3241 catacagttt gtgacctgaa aaagcataga aatcaatatt cagttgctga acatccgcgg
     3301 ggaaatgcac cgccccctga gcaccatcaa ccatcaccac catcccggct gaatgagcaa
     3361 aggtaatcgc tcgcgccaga tccgggcaac cgccagtaac gttcgacatc tgacccaacg
     3421 ccagaatccg actacgggga gtaatcagtt ctggcaacaa atcgacatcc ggcagtcgct
     3481 gcgcattaag cggcaatttc accactttgg ctccagtttg ttgggcgacc atcagccagg
     3541 ggacgaggtt ggcgtggtgt tctgccacgc tgacaataat ctcatcgccc agttgcagac
     3601 gcggacgcgc atagcattgt gcaaccatgt tgatggattc agtggtgccg cgcgtccaga
     3661 cgatagtttt atcatccggt gcattcagta attgcgccac tttctctcgt gcagcttcat
     3721 aacgcgcggt caggcgttgg gcttcggcaa actggctgcg atggacgttt ccggcgctca
     3781 ggctgtaaaa ctgtcgggtg gcttcaacca cggcttcagg tttaagcgcg gtcgcggcgc
     3841 tgtcgagata gacgcccgca tcctgtagag cgggaaactg ggcgcgaaac tgcgcgggat
     3901 taaaaacgtt catggtactc ctcggcttga tgcaccgatc gtgacgcaaa attcactttg
     3961 atacaaggag agtgctaacc atcagaatta tctggatatc ctggcgaagc cgcgtaagta
     4021 acatatgctg aatagtgtta ggcgttaatt ttagcctgtt aatcaatact attaagattt
     4081 attaatcgct ttaaggagaa gatgatgaaa aagactgccg caattatttc tgcctgtatg
     4141 ctgacttttg ccctgagcgc ctgttccggt tcgaactatg tgatgcacac caatgacgga
     4201 cgtaccatcg tctctgacgg caaaccacag actgataacg ataccggtat gatttcgtat
     4261 aaagacgcta atggcaacaa acagcagatc aaccgtactg acgtgaaaga gatggtcgaa
     4321 ctggatcagt aattcacgat cgcaaggtaa aaaaaagcac cgcaattagg cggtgctaca
     4381 ttaatcacta tggacagaca gggtaaatgt acaggaagtg aaaaaaggta gctttgctac
     4441 catggtctga atcgcagacc aattgcaaac acaacaacac aacatcacaa ccgtaagcca
     4501 aaagttcacc agaacacgca ttccgataaa acttttcgtt ccggctcagg aagtgccgcc
     4561 actataggta tttgctggta gaagctcaac ggacaattta taatggctca gattaaaaaa
     4621 actaataggt tacacagtgt gatctaattg ttaaattcat ttaacatcaa agtttaaaag
     4681 ccatgtctaa acgattacca ccgctaaatg ccttacgagt ttttgatgcc gcagcacgcc
     4741 atttaagttt cactcgcgca gcagaagagc tttttgtgac ccaggccgca gtaagtcatc
     4801 aaatcaagtc tcttgaggat tttttggggc taaaactgtt ccgccgccgt aatcgttcac
     4861 tcctgctgac cgaggaaggg caaagctatt tcctcgatat caaagagata ttttcgcaat
     4921 taaccgaagc gacgcgtaaa ctccaggccc gtagcgccaa gggggcgttg acggtcagtt
     4981 tactccccag tttcgccatt cattggttgg ttccgcgact ttccagcttt aattcagctt
     5041 atccgggaat tgacgttcga atccaggcgg ttgatcgtca ggaagataag ctggcggatg
     5101 atgttgatgt ggcgatattt tatggtcggg gcaactggcc ggggctacgg gtggaaaaac
     5161 tgtacgccga atatttattg ccggtgtgtt cgccgctact gctgacaggc gaaaaaccct
     5221 tgaagacccc ggaagatctg gctaaacata cgttattaca tgatgcgtca cgccgtgact
     5281 ggcagacata tacccgacag ttggggttaa atcatatcaa cgttcagcaa gggccaattt
     5341 ttagtcatag cgccatggtg ctgcaagcgg ctattcacgg gcagggagtg gcgctggcaa
     5401 ataacgtgat ggcgcaatct gaaattgaag ccggacgtct tgtttgcccg tttaatgatg
     5461 ttctggtcag taaaaacgct ttttatctgg tttgtcatga cagccaggca gaactgggta
     5521 aaatagccgc ctttcgccaa tggatcctgg cgaaagccgc tgctgaacaa gaaaaattcc
     5581 gctttcgtta tgaacaataa tttacgtagg gtacgaccat gaccagccgt tttatgctga
     5641 ttttcgccgc cattagcggc ttcatttttg tggctctggg cgcttttggc gcgcatgtgt
     5701 taagtaaaac catgggggcc gttgagatgg gctggatcca gaccggcctc gaataccagg
     5761 cgtttcatac gctggcgatc ttaggtctgg cggtggcaat gcagcgtcgc atcagtatct
     5821 ggttttactg gagtagcgtt ttcctcgcgt taggcacggt gctgttcagc ggcagccttt
     5881 attgcctggc actgtcccat ctgcgtttgt gggcgtttgt cactccggtt ggcggcgtga
     5941 gcttccttgc gggctgggcg ttaatgttag ttggtgctat ccgtttaaag cgcaagggcg
     6001 taagtcatga ataaggttgt attgctgtgc cgtccgggct ttgaaaaaga gtgcgccgca
     6061 gaaattaccg ataaagccgg ccagcgggaa attttcggtt ttgcccgcgt gaaagagaat
     6121 gcgggttatg tcatttatga atgttatcaa cctgatgatg gcgataagtt aatccgtgag
     6181 ctgccgttca gttcattaat ttttgcccgc cagtggtttg tggtggggga actcctgcag
     6241 catttgccgc cagaagatcg tattaccccc attgtcggca tgttacaggg cgtagtagag
     6301 aagggcggtg aactgcgtgt tgaagttgcc gataccaacg aaagcaaaga gttgctgaaa
     6361 ttctgccgta aattcaccgt gccgctacgc gctgccttgc gcgatgccgg ggtgctggcg
     6421 aactatgaaa cgccgaagcg tccggttgtg catgtattct tcattgcacc aggttgctgc
     6481 tataccggtt actcatacag caacaataat tcgccgttct atatgggcat tccgcgcctg
     6541 aaatttccgg cagatgcgcc gagtcgttcc acactcaaac tggaagaggc atttcatgtg
     6601 tttattcctg cggatgagtg ggatgaacgc ctggcgaacg ggatgtgggc ggtggatttg
     6661 ggcgcttgcc caggcggctg gacctaccaa ctggtgaagc gcaacatgtg ggtttattcc
     6721 gtcgacaacg gcccgatggc gcaaagtctg atggataccg ggcaggtgac gtggctgcgg
     6781 gaagacggtt tcaaattccg tccgacacgc agcaatatct cctggatggt atgcgatatg
     6841 gttgaaaaac cggctaaagt tgcggcattg atggcgcagt ggctggttaa tggctggtgc
     6901 cgtgaaacca ttttcaatct caaactgccg atgaaaaaac gctacgaaga agtatcacac
     6961 aatctggcgt atattcaggc acagcttgat gaacatggca taaatgctca gattcaggcg
     7021 cggcagttgt atcacgatcg cgaagaagtg acggtgcacg tccgccgcat ctgggctgcg
     7081 gtgggtggtc gtcgcgacga gcgataacag gcaaggccgg ggcgtcgtct ccggcctgct
     7141 accctttccg tcaggctgtt accaaagaag ttgcaacctg gcgctcatca gaaataatgt
     7201 gcgttacctc atccagatgc ccgatgcggg cttcccctga acgattaaat ttacttttat
     7261 caatcaataa caacgattgc gcggcacgtt ttaatagcat cgatttgtaa tcagcgttga
     7321 tggcattgga gtcccacagt gcgccgctgc tatcgatccc ttcacaagaa aaaataaaca
     7381 gatcgatttc cagcgattta agttgggaaa tcagcgaggg attgacgtaa cagccatatt
     7441 tgcgctcaag tgtgccgccg gaactgatca gttgaatgcg ttcgcgttta ccgagttcat
     7501 ggcaaatcgg atggctattg gtgaagacct gaatgttgat gtcaggcaac tggcgtgcca
     7561 gataccagca agttgaactg gcgtctaagg ctatcaccat cccttcttca atccacgcga
     7621 gcgcctcgcg cgcgatatct gctttatgcg cataatggct tttcagccgg atgtgaaagg
     7681 gatcgccact gtcttgattt tgacggtgga tatatttagc gcgtccatga ttgcgcagaa
     7741 ttttaccctg cgtctgtaat tcattgagat cgcgacgaat ggtttcttta ctgaccttta
     7801 gctgttcaga gagagcttcc gtggtcaggc tggtatggtt cagcagcagg tcgactatcg
     7861 cttgctggcg tgccgctttc atatttttca ccccatcatt cgataaccgc ccgcgtcatg
     7921 cgggcgtacc ggtatgagat tacggtgtta cccctttttt taaaagaata ttcccgtact
     7981 tcgctcgttc gcctgtgata acgatcgcaa aggctttttg cgcccgttca taaaacgcaa
     8041 aacgattgat gcggatgatg tcaggacacg gggcttgtag tgaaagcgca ttacggtaac
     8101 gtcgttctac ttcaggatcg agagtgtcac cttctaccgc tgccatcatc accagcggcg
     8161 gggcataact gtccagctca aataacggga taatcgcctg gagcaagtcg ctcaccaaca
     8221 ggccatcagc gcggatcacc tgcggtccca tcgaatgggc gggaaagtga gcatcggaaa
     8281 aaataatttc atctccatgt cccatctctg ccagcacttt caatagttcg ggagaaatta
     8341 acggcgaaat tgttttcagc atttcacact tcctctataa attcaggttc agtttgcggg
     8401 tagaaataac ggtactgata atgaatctgt gcacgggctt cttccgggct gttgaattcc
     8461 cctacgccat accaaccgaa cagcgcagct cctgcgacgg tcgtttcggc atcgtcgaga
     8521 acttttaccg gaatatcaag catattggct ttaatctgat tccacaatgt gttgcgactt
     8581 cctccaccga ctaacaataa ttcagaggcc ttaaagtgcc cgattttttc cagcatctgt
     8641 agattgcgct gtaattgcgc agttaaccct tccagcgccg cgcgatagaa atgcccccgc
     8701 gtggtattaa gcgtcactcc ttgccagcca gcgttctgac acgacaataa atcacactgc
     8761 atttttacgc catccgcgcc aggcgcgatc agacgagctt cttcaatcaa catttgccag
     8821 ggtgtttcag ccgtccagaa cagttttctc acccattcca gcacgccgga tgccagccat
     8881 tgcatacctg ggttatacaa ccctgcctgg ctatccagtt cgcaggtgga accggcgtac
     8941 tgacttaaca gcgaagtatc aacctgggcg ctacggacca ttaaaatttc ccatgtaccg
     9001 gaagagagca cgggttcatt ttggtcggca ccagcgccaa aaagggcgaa ctgggtatcg
     9061 tgacctgcgg aaatcaccgg tatgccaacg ggtaagccga gcattgctgc ggcgctgttc
     9121 tgtagcgtac caatctgttc acccgcttcc accagacgag ggaagagtcg gcgtggaata
     9181 ccggtggctt gtaaaatttg cggactgaaa tcgcgttgct ggatatccag catctggctg
     9241 gttccggcca tcgtgatatc agtagtgaat tcgccggtta aacggtggtt aatcagcgac
     9301 gaaataaaga gccaggcgtg cgcgcgttcc agcagttgtg gatgattttc tttcaaccac
     9361 accaacttat ataacgtatt gaaactaaag gctccgacgc cagaaatagc ctgcaaccgc
     9421 tgtgcggaga tcaactgttc aatattgtcc ataaccgctg ctgttcgcgg acatttccag
     9481 ctaatgatcg gatagagcag attgccttgc ttatctacca gagcgccatc cacaccaaag
     9541 gtggtgacgg cgataccgcg gatgtggcat tcagtcagtt cactattgat ttgccgacag
     9601 caatcagcaa agcgttgcaa aatggcgtct aaagaccact ggtgccaggt gttgttttcc
     9661 atcgcgatat cgctggcatt aggcgttgag gcgcgggcaa caatttttcc ctgccgatta
     9721 accgcgatgg ccctgacatt ggtcgcgcca cagtcgagta ccaggataac ttcttgtttc
     9781 ataatcgctc ctgcaatata gccggataac attgcttatc cggctaacca ctcttgcttg
     9841 ttcaggcccg gtagccggag cgaccgggca tcacatcagg gagtaatgta ttaacgcttg
     9901 tacaacgggc cgtagttctg gcaagcgcgg taatcctggc cttcaatatc catgccgtgc
     9961 gcagcccagg cagaaggacg atacactttg gtctcttcaa cgttgtgcat acataccggg
    10021 atacgcagca tggaggcgag agtgataaag tctgcgccaa cgtggccgat ggtcagaacc
    10081 ccatggttag cgccccagtt cgccattacc gagtacacat ccgtaaacgg ccctttaccg
    10141 gtgaggcgcg gtgcaaacca ggtggttggc caggttgagt tggtgcgttt gttgaggatg
    10201 tcatgcacat ccttcggcaa ttccacgctc cagccttccg cgatttgcag taccggtccc
    10261 aggcctttga tgatgttgac acgggtcatg gtgaacggga cgccgccttc ggtaaggaag
    10321 cgggaagagt aaccgccgcc acggaagtat tcgtggatcg ccgggcacca ttcggtagcg
    10381 gcgaggcaag cgtcagcctc ttgctgagag atttcccagt gcggcttcat cgtcgggtta
    10441 ccttcgctgt cgcgttgttt acaggaaccg tccagcgcag cagaaccgga gttgatcaaa
    10501 tggatgatgc cgtgttctgc cagtccatcg agtttatgcc ccgttacacg ctcaattgct
    10561 tctggtgacc agtaggtacg cacatcggca aatacctgag cggtgccggt gagctggtga
    10621 cccattagca ttgccacgcc gttaagactg tcgttttcgg tcgccacgac aaagggttcg
    10681 cgcacgccat tccagtcaaa tgaactgtta aggatcgctt cggcggtgtc accattggga
    10741 tattgatcgg tccagtgacg ttgcccctgg aagcccgcag cgatggcgtt gtagccaagt
    10801 gattcttcca cgcgaccaat atcggccagt ttgctgttgc cttgcatcat gtcgcggata
    10861 cacatcgcca tcagtaaact ttcgcgcaga actgcgcggc gttgctcggc attgcgttga
    10921 tactgtttgt tattttcatc ttcgccatag cggaagtttt tatcagccca ggccagtgcc
    10981 atttccagtt cggcttcgtc gtaaatcttc tgatcgatac ggcgacgcag ttcggtcata
    11041 tccaccgcct ggactttcat tcccagccag gattcaaaga agttgtgatc aacaatggaa
    11101 ccggcgatac ccatcgaaac gccgcccagc gacagatagc ttttaccttt catgctggcg
    11161 acggccaaac cggcgcgggc aaagcgcagc agtttttctt caacatcggc aggaatcgat
    11221 gtgtcatcgg catcctgaac gtcatgaccg taaatggaga atgccgggat gcctttctgg
    11281 ctgtgagctg ccagagccgc tgccaggtaa acagcgccgg gacgttcagt gccgttaaag
    11341 ccccaaatgg ctttcgggcg ggttggatcc atgtcgatgg tttcactgcc atagcaccag
    11401 caaggcgtta ccgtaatggt gaggcctaca ttctgactgc tgaatttttc ttcgcaagca
    11461 gcggcttcag ccatacccgc gatacaggta tcggaaatga cacactcgac ggcagctccg
    11521 caggcatggc gcagtttctc ggtcagcagt gcggccgtag ctttcgccat attcattgtt
    11581 tgttcttcaa gcgactcacg aacacccatg cgacgaccgt caataaccgg gcggatacca
    11641 attttcggta agctgatttt tttcattaca gattcctcac tttattcgga aaatatgttc
    11701 agttagttgc cgtttgagag cggaaacggg caaagataaa gatgaccgcg aagcagagtg
    11761 cggggatcag ttcagcagtg gggatgttgc ccgccgcgtc actgacaaaa cccatgaccg
    11821 gagtgacaat accgccgcca atgatggtca taacgatgaa ggacgaaccg tatttggtgt
    11881 cctggccgag attcttaatg cccagcgaga agattgttgg gtactgaatc gacataaagg
    11941 cgctgcataa agtcagggct attaagccca catgaccgcc agcgaaggct gagatcaggc
    12001 acagtgccat agcgattaat gcgtaggcgg ccaggacttt gtgtggtgcg aagcgactga
    12061 tgagccaggt accggtgaaa cgaccaataa agaagcacac catggttccg gttaaatagt
    12121 tagcggcaaa gcctgcagtc atacctggaa tttcttctac agcgtagcga atcaaatagc
    12181 tccagcaggc cgtttgtgcg ccaacgtagc agaattgcgc taatactgcc cagcgccagt
    12241 ggcgaatacg cgccaggcga gaaagcgatg cggagaacga tccttgtttg gcgtcactgt
    12301 gattatcact ctgcaatgcc gggaatttcg tcagcatgat cagcagggcg accagtaaca
    12361 cgatagccac gatgatcata taaggtgtct gtaccgataa taccaggctg tgtttatacg
    12421 cactcaattg ctctggagac attttatcga gaacgtcttg cgattgatgt ggcacgttag
    12481 acaaaataag actttgccca aagacaaccg cgataattgc gccaaacgag ttaaatgttt
    12541 gcgcaagatt taagcggaag tgaccactgc tttccggccc taataccgta acaaaagggt
    12601 ttgcggcagt ttccagacaa cctaatccgg ctgcaataat aaataggcca actaaaaata
    12661 aggtgtagtt cattatttct gcagcgggcc agaataatgc agcgcccaag gcatataaaa
    12721 ataatccggt aataatccct gctttataac tgagtttttt catcaatatc ccagcaggga
    12781 ttgggataat gaaataacca aagtaaaagg ccgattggat caggccagcc tggaaatttg
    12841 tcagcgtaaa agcctgctgg aattgaggta ataaaatgtc gttaaggtta ttggctaccg
    12901 cccaaagaaa aaacagtgag cacagcagcg cgaatggaat aatgtaactt ctgctttgcc
    12961 ctgcatcttt atctaccgca cggtaactct gcgtttgtat tgatgtgttt cccatagcat
    13021 cctcttaggt tcagaagctt aagcacgtaa tcggttatgt tttcaaaagt gcaaaaccag
    13081 cgagttcggg cttcatatca gtgaatattt gcctgtctga aatgaactca tggattcatt
    13141 ttcaaataaa aaacgctctg taatatgacg gcggtcacac ttattagctg gaaggtgact
    13201 ttatgtgact accatcactt taataatcga aatttcatgc ccgtttcaga aaataaatca
    13261 taaaaaacac aaaccagatg accgtattaa ttgtttatgc cgaaaacggg cattcctggc
    13321 ttgagtgaat agaaaaatga ccgttttgat aaatattttt aagagcggtc atttttaatg
    13381 gggttataaa aagagaactt gcagttattt actgtgatta gttgaaccag gtcacaaaat
    13441 ataagcttgt gaatttcttt ctggttaatc aggaagaaga tggtgggtaa ttaaacggct
    13501 aattcaatag tgtgaaagga acaacattat tgccctgttt tgaatcagag agaggtagct
    13561 aatggaacga aataaacttg ctcgtcagat tattgacact tgcctggaaa tgacccgcct
    13621 gggactgaac caggggacag cggggaacgt cagtgtacgt tatcaggatg ggatgctgat
    13681 tacgcctaca ggcattccat atgaaaaact gacggagtcg catattgtct ttattgatgg
    13741 caacggtaaa catgaggaag gaaagctccc ctcaagcgaa tggcgtttcc atatggcggc
    13801 ctatcaaagc agaccggatg ccaacgcggt tgttcacaat catgccgttc attgcacggc
    13861 agtttccatt cttaaccgat cgatccccgc tattcactac atgattgcgg cggctggcgg
    13921 taattctatt ccttgcgcgc cttatgcgac ctttggaaca cgtgaacttt ctgaacatgt
    13981 tgcgcaggct ctcaaaaatc gtaaggcaac tttgttacaa catcatgggc ttatcgcttg
    14041 tgaggtgaat ctggaaaaag cgttatggct ggcgcatgaa gttgaagtgc tggcgcaact
    14101 ttacctgacg accctggcga ttacggaccc ggtgccagtg ctgagcgatg aagagattgc
    14161 cgtagtgctg gagaaattca aaacctatgg gttacgaatt gaagagtaat ttcgtaaagc
    14221 aacaaggaga aggatgatgg ctaacagaat gattctgaac gaaacggcat ggtttggtcg
    14281 gggtgctgtt ggggctttaa ccgatgaggt gaaacgccgt ggttatcaga aggcgctgat
    14341 cgtcaccgat aaaacgctgg tgcaatgcgg cgtggtggcg aaagtgaccg ataagatgga
    14401 tgctgcaggg ctggcatggg cgatttacga cggcgtagtg cccaacccaa caattactgt
    14461 cgtcaaagaa gggctcggtg tattccagaa tagcggcgcg gattacctga tcgctattgg
    14521 tggtggttct ccacaggata cttgtaaagc gattggcatt atcagcaaca acccggagtt
    14581 tgccgatgtg cgtagcctgg aaggactttc cccgaccaat aaacccagtg taccgattct
    14641 ggcaatcccc accacagcag gcactgcggc agaagtgacc attaactacg tgatcactga
    14701 cgaagaaaaa cggcgcaagt ttgtttgcgt tgatccgcat gatatcccgc aggtggcgtt
    14761 tattgacgct gacatgatgg atggtatgcc tccagcgctg aaagctgcga cgggtgtcga
    14821 tgcgctcact catgctattg aggggtatat tacccgtggc gcgtgggcgc taaccgatgc
    14881 actgcacatt aaagcgattg aaatcattgc tggggcgctg cgaggatcgg ttgctggtga
    14941 taaggatgcc ggagaagaaa tggcgctcgg gcagtatgtt gcgggtatgg gcttctcgaa
    15001 tgttgggtta gggttggtgc atggtatggc gcatccactg ggcgcgtttt ataacactcc
    15061 acacggtgtt gcaaacgcca tcctgctacc gcatgtcatg cgctataacg ctgactttac
    15121 cggtgagaag taccgcgata tcgcgcgcgt tatgggcgtg aaagtggaag gtatgagcct
    15181 ggaagaggcg cgtaatgccg ctgttgaagc ggtgtttgct ctcaaccgtg atgtcggtat
    15241 tccgccacat ttgcgtgatg ttggggtacg caaggaagac attccggcac tggcgcaggc
    15301 ggcactggat gatgtttgta ccggtggcaa cccgcgtgaa gcaacgcttg aggatattgt
    15361 agagctttac cataccgcct ggtaaatgcg ctgatgtgat aatgccggat acgacgtttg
    15421 cggcgtatcc ggctcgccgt taccgtacca accgcaattg ctgtaaattg ccgtcgatat
    15481 gcaaatcggt ttgtaagcgg gcaatatcgc ggcacagaaa cgccatctct ttatgggttt
    15541 ctaatttttt gcgccacttt tcggcaaccg catccagatt ctcatatatc ccttccagac
    15601 tctgaaactc gaccagcagc tgcgtggcgc tttttggtcc gattcccgca acacccggta
    15661 cctttgaact gctgatcccc gcaagtcccc agtaatcggg caactgctgc ggttgaacgc
    15721 caaattcttt atcgataaat ggcgcatcca gccaacgttt ctggaagtaa tcacgaatac
    15781 gtaatgtcgg tgaaagtaac tgacagtagc ctttatctgt cgaaacaatc gttgcctgat
    15841 gcccggcctg tgtcactttg accgccagcg tggcggctaa gtcatcggct tcgttgccgc
    15901 tggttgacca gcacgggacg ccgcgttgct caaaggcggc gcgtaatgca ggcatctcgt
    15961 cgtgcaactc ttccggcatt ggcggtcgac ccgctttgta atctggtaaa cgctgatgac
    16021 gccagccgct actgcggttt tcatcatcaa aaacggcgac cgcgtgggtt ggctggctgt
    16081 gcataatgag ctgatcgagc gcatgctggc aggtttcgac acagggcgat ccctgaacgg
    16141 catgaatgcg acgaataaga ttcagtgcat cgacaataag caaatgaaca gccacgataa
    16201 cccccttaaa gatcaggcga taagggtaac attcgatgcc ggaacaggct acggctggat
    16261 gagaaatcgg gaagaggcct cgcaaaacga ggcctctgga gagcgattaa tcgcaggcaa
    16321 cgatcttcat tgccaggccg ccgcgagagg tttcgcggta cttggcgttc atatctttac
    16381 ctgtttcgta catggtttcg ataactttat cgaggcagac gcgcggctcg ctggtacggc
    16441 gtagcgccat acgtgcggcg ttcaccgctt ttactgccgc aatggcgtta cgctcgatgc
    16501 atggcacctg gacctgtccg gcgaccgggt cacacgtcag accgaggttg tgctccatgg
    16561 cgatttccgc cgcgatgcac acctgcgccg ggcttgcacc taacagttct gccagaccag
    16621 ccgccgccat tgagcacgcc acaccaactt caccctggca gccgacttca gcaccagaaa
    16681 ttgacgcgtt catcttataa agagaaccaa tggcactggc taccagcagg taacgagcca
    16741 gtgagttagc gttcacttcg cggataaact tgtcgtagta cgccagcact gccgggataa
    16801 tcccgcacgc accgttagtc ggcgcagtca ccacgcgtcc gccagcagcg ttctcttcgt
    16861 tcactgccaa tgcaaacatg ttgatccagt caacaaccgc catcgggtca gtggtggttt
    16921 tatcctggct gaccagcatc cggcgtagtg ccgcagcacg gcgtggaacg cgcagtttgc
    16981 caggcaacac gccttcggtg gaaataccgc gctcaatacc gccacgcatg acttcccata
    17041 cgttcgccag gtgctgttcc agctcttctt tgctgtgcag cgccagctca tttttcatca
    17101 tcaggccaga gagtgacagc ccggtttcct gacaatgttt ttgcagatcg gctgctgaac
    17161 tgtacggata aggaacttca accggtgcgc tattctgctg gccaaaatgc tcttcatcaa
    17221 cgataaagcc accgccaata gagtagtaag tctggctgta aacgacttta tcgcccgcca
    17281 gcgcggtaat gcgcataccg ttttcatgca gagaaaggtt gtctgcatgg aagttcatgc
    17341 actgatcaac cgggaactcc acttcatgct gaccgtttgc cagcatcagg cgaccatgag
    17401 tattcacatc ctgaataaaa ccagggatgg aatcgatatc cacggtatcc ggcaggttac
    17461 ccgccaggcc cataataatg gcgatatcag tgtggtggcc tttaccagtc agagagagcg
    17521 agccgtacac gtcaaccacc acgcgggtca cgtctttaag caggttacgg gcaatcagat
    17581 cgtcggtaaa ttgtttaccc gctttcattg gtccaacggt atgagaactg gaagggccaa
    17641 tgccgatttt gaaaatatcg aatacgctaa tcataggaaa tacatcgcgt taaaacggag
    17701 gaagcgccgc ccgaaagcgg cgcgaaagga cttagctgaa cagagagtag aagattgcgg
    17761 agattgcaat cagacccatc acgacaacga atacgttgct gatgtgaccg ctgtacttac
    17821 gcattgccgg tactttctga attgcgtaca tcggcatcag gaacaggatc atcgcgatga
    17881 ttgggccgcc cagggtttca atcataccca ggatgctcgg gttcagggtg gcaacaatcc
    17941 aggtcgttac cagcatgaac agcgcagtga tacggttcag cttgttgatt tcgatagact
    18001 tacctttacc acgcagagat ttaatcacca taccgttgaa gccttcacgt gcgcccaggt
    18061 agtggccgag gaaggatttg gtgatagcga taatcgcgat aatcggagcc atccacgcga
    18121 taaccggtgc gttaaagtgg ttagctaggt aagacagaat cgagatgttc tgctctttag
    18181 ccgcagccag gtctgccgga gtcaggctca gtacgcagct gaagacgaag aacattacgg
    18241 tcagcaccat catgatgtgt gcgaatgcca ggatcttcga gcatttctgt tctgccatat
    18301 cgccgtactc ttcacgcttc gcaacggcga aagaagagat gatcggagag tggttgaacg
    18361 agaacaccat taccggaatt gccagccaca gggtcatcca cagaccgttt ccggttgcag
    18421 atgcagtgtc cagagacagc gtttccagtg ctgcgccgtt ccactgcggg atcaggtaca
    18481 gagccagcag catcagtacg ccaacaaacg ggaataccag aatactcatc gctttaacga
    18541 tcatctgctc accaaagcga acgatggtca tcatacccac gatcaggatc agcgacagaa
    18601 tcgcacgcgg cggtggcgtc atacccagct ggtgagacat gaagctttca actgtattgg
    18661 tgattgccac gctataaacc agcaggatcg ggtagatagc gaagaagtag agcagggtaa
    18721 tcagtttacc tgcgccaata ccaaagtgtt cttctacaac ctcggtgatg tcttcgcccg
    18781 ggtttttacc agacagtacg aagcgagtca ggccgcggtg agcaaaaaac gtcatcggga
    18841 acgcaaggat agccatgatg atcagcggga tcataccacc aacaccggcg ttgattggca
    18901 ggaacagcac gcccgcgccg attgccgtgc cgtaaaggcc cagcatccac atggtgtctg
    18961 tcttgcgcca ggcactacga gagtctttcg acgcaatcgt gctggtttga gtcgtttcca
    19021 tctatttctc ctggaggaat gcaaataatt cagcttttag ccagttcagc ccgggaaggt
    19081 gcctgaccgt aaaaatgaaa tctgtctatc ggcgatggct taatcattta ccgccgtaat
    19141 aatttgaggg cggaaagata cacacaagtt atgtatctat cagtgatctt gatcccaaat
    19201 acaataatct acttggaaga tagattatcc caacgcttta ctctgtaaat caaaagtaaa
    19261 agagaattta agggcaatat ttgcccatta aacgacatct gacttcttcc ggggcgcgca
    19321 atatatgtag gttgaactga aaaaatcaat acttttagtt aatcatggcg ataatttctt
    19381 tttcatagcg ataaaatacg gtttatgaaa atagggaaac gtttgcgttg caatataaat
    19441 tgtatgacat attaactgtg atgatatttg tcgcgtgagg gtggtaagaa acattcatat
    19501 aacaaatgaa atgagttgat gagtaagtga ccaaaaacga gaaaacccgg caacaaacgt
    19561 caccggggga ggggagatta cgtgcagatt tcgtagcaag ggatgtaggc tgagcctggc
    19621 aacttcatac gatgctgggc aacaaaaccc tgtagcaggt cgtccatacg acgcataatc
    19681 tctttatcgc cgttgatttt gtaaggacca aactcttcaa tggcgcgaat accgacttct
    19741 tttacgttac ccgccacaat accggagaac gcacggcgca ggtcggcagc cagcacttca
    19801 acaggttgat ccgggtaaag cttcagatta gccatattct cgtgagacgg ctcaaacggc
    19861 atttgcaaat ctggtgcaat gcgcattgac cagttaaagc tgtaagcgtc accagtgtcg
    19921 cggcgatttt ctttcaccag cggcatcgat tttttcatct gacgagcgac ttcagcggcg
    19981 tcatcaatga tgatgcggta atggcggcgc gcgttttcgc ccagcgtatg tacgacaaac
    20041 tcgtccagta cgcggaagta gtcggcgctc tctttcgggc cggtgaggat caatggtaaa
    20101 acctgatctt tgttggccgg gttcattaaa attcccagca aatacagcaa ctcttctgcc
    20161 gtacccacac cgccagggaa gataatgata ccgtgagcga tacggacaaa cgcttccaga
    20221 cgtttttcga tatctggcat gatgatcaat tcgttgacca gcgggttagg cggttcagcg
    20281 gcgataatcg acggctcggt cataccaata aaacgactgt ctttgtaacg ctgctgcgcg
    20341 tgtccgaccg cagcaccttt catcggcgct tccatcgctc ccggaccaca gccggtgcag
    20401 atattcagct cacgcaggcc cagctggttt ccgacgcgac gggcatacaa atactcgttt
    20461 tcgttaattg agtgaccgcc ccagcagacc accatatttg gcgcttcacc cacatgcagc
    20521 gcgcgagcgt tacgcaagat ggaaaagacc aggttagtga tgtggacgga gttgtcgaga
    20581 ttcaggttgg gaaaacgaac ggtattatgg atttgcccgt aaacgaagag aatgtctcgc
    20641 agaaccgcga acaagttggc ctgcaaagcg cgaataattc gcccatcgac aaaagcctct
    20701 tccgggggat taatcagttc cagctttacg ccgcgttcac ggcgcaggac gttaatatcg
    20761 aaattttcaa aacgagacag caattctttg ctgttatcgg tcaaactacc ggagttcagt
    20821 acggcaagtg aacagttgcg aaacagttga tagaggtcgc tgctggcggt gcgtttaagc
    20881 atatccactt ccagctgcga caacatatcc atggagccaa gcgggctaat atgtgtaatc
    20941 aagaaaaact ccttatggga cgaatatcgt cattcccttt gattacaata gccctgcctg
    21001 atgacctttc acaaccttct gcgtgaatcc agcacgcaaa attgagaaaa aatttattgc
    21061 cgaaccagtc ttgtggtcga tgggacaaaa tcgctattac tgcgccacgg gttaatgtcc
    21121 agaccgccac gacgggtata acgtgcgtaa acgcttaatt tctctggctg gcagaagcgt
    21181 aacaggtcat taaagatgcg ttccacgcac tgttcgtgga actcgttgtg atgacggaat
    21241 gagaccaggt aacgcagcag tttttctctg tcaatttggc gtccacgata ctgaatttgg
    21301 atcgaacccc aatctggttg atgggtgatc aggcagtttg atttcagcag gtggctgaca
    21361 agcgtctctt ccactacttt ttcaccgctg gtggcattct ccagatagtc agtagtgaat
    21421 tcatagttat cgatagtgat atcctggtca tcaatgcagg tgccattgaa atgacctatc
    21481 ggctggcctt ccagttcatc aagacgatat aacgccacgc taaccttacc ctgggcgcaa
    21541 gtgcttaagt cgcgctccag cgtctggcgc acctcatccc agttattaaa acgcgtctgg
    21601 ttaaaactgt tgagatagag cttaaaactc ttcgactcaa tcagatttgc gctggtgtaa
    21661 tcaagttcaa cgtgaccgac ggcaacctgc ggcaaacctt tcgcattcag ccaggaaagt
    21721 tcatacagcg tccagatatc cgtaccgtga aaaggcaggt tatccgcttt cagacccagc
    21781 gggtcgcgat tcaggctgcg tggaacgcct tgcagtaggt tggcgtcata ggtatcccgg
    21841 taatcggttg attttccaag agtcaggccc gcaagtgcct gatggtttgc ataagaagac
    21901 atgtttcacc gtcataaacc agataaacta aacgacaagt gtatctggtt taagtgaaga
    21961 gagaaattag tggacgattt gaccgcacaa gccctgaaag attttactgc acgctactgc
    22021 gatgcgtggc atgaagagca taaaagctgg ccgttaagtg aggaactgta cggtgttcct
    22081 tcaccgtgca ttatttcaac taccgaagat gccgtatact ggcaacccca gccgtttacc
    22141 ggggaacaaa atgtaaacgc ggttgaacgc gcttttgata ttgtgataca acccacaatt
    22201 catacctttt atacgactca gtttgccggg gatatgcacg cgcagtttgg cgatatcaaa
    22261 ctgacattgc tgcaaacctg gagtgaagac gacttccgtc gggtgcagga aaatctgatt
    22321 ggtcatctgg tgactcagaa aaggctaaaa ctgccgccca cgctatttat cgcgacgctg
    22381 gaagaagagt tagaggtcat ctcggtgtgt aatctctccg gtgaagtgtg caaagagacg
    22441 ctgggaacac gtaaacggac gcatttagcc tcaaatcttg cggaattcct taatcaactt
    22501 aagcctcttc tgtaatccaa ataccccatc tggttgtgag agatctctta cagactctgt
    22561 aggagatcgc caggaaataa gcgaatactt aaaaagataa gaatcgttat tttcatttaa
    22621 aatcaaaatg ttgattgtta attcttaact ttcatatgaa attttcctta aggcattgtc
    22681 tgtaagcgcc ttgtaagact tcgcgaaaaa gacgattcta tcttcgtcga cagggagtca
    22741 gacaacgaag tgaacatcag gatgatgaca cttctgcagg acacaccagg atggtgtttc
    22801 agggaaaggc ttctggatga agcgaagagg atgacgcagg acgcgttaaa ggacacctcc
    22861 aggatggaga atgagaaccg gtcaggatga ttcggtgggt caggaaggcc agggacactt
    22921 caggatgaag tatcacatcg gggtggtgtg agcaggaagc aatagttcag gatgaacgat
    22981 tggccgcaag gccagaggaa aagttgtcaa ggatgagcag ggagcaacaa aagtagctgg
    23041 aatgctgcga aacgaaccgg gagcgctgtg aatacagtgc tccctttttt tattcctgct
    23101 atccttcgcg gcagtttttc ttattgaggt tgctttatga ccactcatga ccgcgttcgt
    23161 ctccagttgc aggcgcttga agcgttactg cgtgaacatc agcactggcg aaacgatgaa
    23221 ccccagccgc atcaatttaa tagtacccaa ccgttcttta tggacaccat ggaaccactg
    23281 gagtggttgc agtgggtgct gatcccgcga atgcacgatt tgttagataa caaacagccg
    23341 ttaccgggcg cttttgccgt tgcaccctat tatgaaatgg cgctggcgac ggaccatccg
    23401 caacgcgcgc ttattctggc tgagctggaa aaactggatg ctctttttgc ggatgatgcg
    23461 agctaatgct ggaaatactc tatcaggatg aatggctggt tgcggtaaat aaaccctccg
    23521 gctggctggt tcaccgcagc tggctggatc gcgacgagaa agtagtggtc atgcaaaccg
    23581 tgcgtgatca gattggccag cacgttttta ccgctcatcg tctggaccga cccacttctg
    23641 gtgtgttgtt gatgggatta tccagcgagg ccggacggct gctggcacaa cagtttgaac
    23701 agcaccaaat ccagaaacgt taccatgcga ttgtgcgcgg ctggttgatg gaagaagcag
    23761 ttctggatta tccactggtg gaagaactgg acaaaatcgc tgataaattt gcccgcgaag
    23821 ataaaggctc gcagccagca gtgacgcatt atcgcggtct ggcgaccgta gaaatgccgg
    23881 tagcgaccgg acgttacccg accacgcgct acggcctggt ggaactggag ccgaaaaccg
    23941 gacgcaaaca tcagcttcgc cgacatcttg cccatttgcg tcatccgatt attggcgata
    24001 gcaaacatgg cgatttacgc cagaatcgca gcggtgctga gcattttggc ctccagcggt
    24061 taatgctaca tgccagtcag ttgtcattga cgcatccttt tactggcgag ccgctgacta
    24121 ttcacgcggg cctggacgac acctggatgc aggcgttatc acaatttggc tggcgcggtc
    24181 tgcttcctga aaatgaaagg gttgagttca gtgcgccatc gggtcaggat ggagagataa
    24241 gttcctaatc cagggagaaa acggtatggc ggaaattggt atttttgtcg gcaccatgta
    24301 cggaaattcg ctgttagtgg cggaagaagc agaagcaatt ctgaccgcac agggccacaa
    24361 agcaacggta tttgaagatc ctgaattaag cgactggctg ccctatcagg ataagtatgt
    24421 tctggtggtt acgtccacga ccgggcaggg cgaccttcct gatagcattg tgccgctctt
    24481 tcagggaatc aaagatagtc tgggcttcca gccgaatctg cgttatggcg tgattgcgct
    24541 cggcgacagc agttatgtaa atttctgcaa tggcggcaaa cagttcgatg ccttgttgca
    24601 ggaacagagc gctcagcggg ttggcgaaat actgttgatt gacgccagtg agaacccgga
    24661 accggaaacg gaatcaaatc cgtgggtcga acagtggggc acgctgttgt cctgatatgt
    24721 tcagcgagcg gtaaatgtcg ttttagcggt gctgaatcga atcttttcag gcaaatgcca
    24781 gtaaaaactg cttcatagcg cggattttta ctggcgtttg cctggagtca agcgatccat
    24841 ttcatactct tctttatttc ttcgttttta cccttccttt cttgttcttg ttttcatttc
    24901 cgtgaagtgg attccaccgt ccagggctaa tgccaaaatc gggcctcatt gaacgcatta
    24961 atgttgtgtt gttgcacggt gagccgctat ggcgcgcttt ttatactgct attgccagat
    25021 ataaacacgc gccgtattcg gcgaacgacc tataaaaacg gcaaaaaaca ccctacgtca
    25081 cctctgattt cctggcgatg tcgcagtcca gagtgagcgt ggctaacgcg aattttcagg
    25141 agtgcaacaa tgagttcttt aagtcaggct gcgagcagtg tggaaaaacg cacgaatgct
    25201 cgttactgga tagtggtgat gttgtttatc gtcacatcct tcaactacgg cgaccgcgct
    25261 acgctttcta ttgctggttc ggaaatggcc aaagatatcg gccttgatcc cgtgggaatg
    25321 ggctatgtgt tctctgcctt ctcatgggct tatgttatcg ggcagatccc tggtggttgg
    25381 ttgctggatc gttttggttc aaaacgcgtc tacttctggt cgatctttat ctggtcgatg
    25441 tttaccttgc tgcaaggctt cgtcgatatc tttagtggat tcggcattat cgttgccctg
    25501 tttacgctgc gcttcctggt cgggcttgct gaagcgccat ctttccccgg caacagtcgc
    25561 attgttgcgg cctggtttcc ggcgcaggaa aggggaacgg cggtgtcgat ttttaactcc
    25621 gctcaatact tcgcaacggt gatcttcgcg ccgattatgg gctggctgac gcatgaagtg
    25681 ggctggtcac acgtcttctt ctttatgggc ggtctgggga ttgtcatcag ctttatctgg
    25741 ttgaaagtca tccacgagcc aaatcaacat ccgggggtaa atcagaaaga gctggagtac
    25801 atcgccgcgg gtggcgcgct gatcaatatg gatcagcaaa acaccaaagt taaagtgccg
    25861 ttcagcgtga agtgggggca gatcaaacaa ctgctcgggt cacggatgat gatcggcgtt
    25921 tatatcggtc agtactgtat caacgccctg acttactttt tcattacctg gtttccggtt
    25981 tatctggtgc aggcgcgtgg gatgtcgatt ctgaaagcgg gctttgtggc ttccgttccg
    26041 gcggtttgcg gttttatcgg cggtgtgctg ggtgggatta tttccgactg gctgatgcgc
    26101 cgcacgggat cgctgaacat tgcgcgtaaa acaccgatcg taatgggcat gttgctgtcg
    26161 atggtgatgg tgttctgcaa ctacgtcaac gttgagtgga tgatcatcgg ctttatggcg
    26221 ctggccttct tcggtaaggg catcggggcg ctgggttggg cagtaatggc agataccgcg
    26281 ccaaaagaga tcagcggtct ttccggtggc ctgttcaaca tgttcggtaa catttctggc
    26341 atcgtcacgc caatcgcaat tggttatatc gttggcacga ctggctcctt taatggggcg
    26401 ctgatttatg ttggtgttca tgccttaatc gcggtactga gctacctggt gctggtgggc
    26461 gatatcaagc gtatcgagct gaaacctgtc gcggggcaat aagatgacga cacaatccag
    26521 tcctgttatt actgatatga aagtcattcc ggtcgccggg cacgatagca tgttgcttaa
    26581 tattggtggg gcacataacg catatttcac ccgcaatatt gtggtactca ccgataacgc
    26641 cgggcatacc ggcattggtg aagcgccggg cggagaggtg atttatcaaa cactggtcga
    26701 tgctattccg atggttctgg gccaggaagt tgcgcgcctg aataaagtgg ttcagcaggt
    26761 gcataaaggt aatcaggcag ccgattttga taccttcggc aaaggtgcct ggacttttga
    26821 attgcgcgtt aatgccgtgg cggcgctgga agccgccttg cttgacctgc taggtaaggc
    26881 gctgaatgtt ccggtctgcg aactgttggg gccaggcaag caacgcgaga ctattaccgt
    26941 cctcggttat ctgttttata tcggtgatcg gaccaaaacc gatcttcctt atctggaaaa
    27001 tacgccgggc aaccatgagt ggtatcagtt gcgccatcag aaagcgatga acagcgaagc
    27061 cgttgtgcgt ctggcggaag cctcacagga tcgctatggc tttaaagatt tcaaacttaa
    27121 gggcggggtg ttacctggcg agcaagaaat cgacactgtt cgggcattga agaaacgctt
    27181 ccctgatgcg cggattaccg ttgatcccaa cggtgcctgg ctacttgatg aagccatttc
    27241 tctatgcaaa gggctgaatg atgttcttac ctatgccgaa gatccatgcg gcgcagaaca
    27301 gggtttctcc ggtcgtgaag tgatggcgga gtttcggcgg gcgaccggct tgcccgtcgc
    27361 gactaacatg atcgccacca actggcgcga aatgggtcat gcggtgatgc tcaatgcggt
    27421 agatattcca cttgccgatc cgcacttctg gacgctttcc ggtgcagtcc gtgtggcgca
    27481 gctttgcgac gactgggggc tgacctgggg ctgccattct aataaccatt tcgatatctc
    27541 tctggcgatg tttacccatg tgggcgcggc ggcaccgggt aatcctaccg ctatcgatac
    27601 ccactggatt tggcaggagg gcgattgtcg cctgaccaaa aatccgctgg agattaaaaa
    27661 cggaaaaatt gccgttcctg atgcgcccgg tctgggcgtg gaactggact gggaacaggt
    27721 acaaaaggca catgaggcct ataaacgtct gcctggcggt gcgcgtaacg acgcaggtcc
    27781 gatgcagtac ctgatccccg gctggacctt tgaccgtaaa cgtcccgttt tcggccgtca
    27841 ttgattctga aaaaggacat aaatatgagt tctcaattta cgacgcctgt tgttactgaa
    27901 atgcaggtca tcccggtggc gggtcatgac agtatgctga tgaatctgag tggtgcacac
    27961 gcaccgttct ttacgcgtaa tattgtgatt atcaaagata attctggtca cactggcgta
    28021 ggggaaattc ccggcggcga gaaaatccgt aaaacgctgg aagatgcgat tccgctggtg
    28081 gtaggtaaaa cgctgggtaa atacaaaaac gttctgacgc tggtgcgtaa tacttttgcc
    28141 gatcgtgatg ctggtgggcg cggtttgcag acatttgatt tgcgtaccac tattcatgta
    28201 gttaccggga tagaagcggc aatgctggat ctgctggggc agcatctggg ggtaaacgtg
    28261 gcatcgctgc tgggcgatgg tcaacagcgt agcgaagtcg aaatgctcgg ttatctgttc
    28321 ttcgtcggtg atcgcaaagc cacaccgctg ccgtatcaaa gccagccgga tgactcatgc
    28381 gactggtatc gcctgcgtca tgaagaagcg atgacgccgg atgcggtggt gcgcctggcg
    28441 gaagcggcgt atgaaaaata tggcttcaac gatttcaaac tgaaaggcgg tgtactggcc
    28501 ggggaagaag aggccgagtc tattgtggca ctggcgcaac gcttcccgca ggcgcgtatt
    28561 acgctcgatc ctaacggtgc ctggtcgctg aacgaagcga ttaaaattgg taaatacctg
    28621 aaagggtcgc tggcttatgc agaagatccg tgtggtgcgg agcaaggttt ctccgggcgt
    28681 gaagtgatgg ctgagttccg tcgcgcaact ggcctgccga ccgcaaccaa tatgatcgcc
    28741 accgactggc ggcagatggg ccatacgctc tccctgcaat ccgttgatat cccgctggcg
    28801 gatccgcatt tctggacgat gcaaggttcg gtacgtgtgg cgcaaatgtg ccatgaattt
    28861 ggcctgacct ggggttcaca ctcaaacaac cacttcgata tttccctggc gatgtttacc
    28921 catgttgccg ccgctgcacc gggtaaaatt actgctattg atacgcactg gatctggcag
    28981 gaaggtaatc agcgcctgac caaagaaccg tttgagatca aaggcggtct ggttcaggtg
    29041 ccggaaaaac cggggctggg tgtagaaatc gatatggatc aagtgatgaa agcccatgag
    29101 ctgtatcaga aacacgggct tggcgcgcgt gacgatgcga tgggaatgca gtatctgatt
    29161 cctggctgga cgttcgataa caagcgcccg tgcatggtgc gttaagacag aaaaccaaaa
    29221 ccggctctat ctggagccgg cttgagactg atgctaaacg caaaatagcc tgttgtgcta
    29281 tgtatcaggt ctacatcatt ttttaattca ttgaatttaa ttggttttgt aggccggata
    29341 aggcgttaac gccgcatccg gcataaacac aggcactttg tcaccaatct gaaaccagcg
    29401 tcataaaaag ccgattgcta ctcgacaaga cgtccgttac ccgagaattt tgctggcttc
    29461 gcgcgctaca ttatccatct cgtccaacag ttccagcagc tccggctcca aatcttcttc
    29521 tttagtaccg ctacgtagct gttgttcgat cagttggcag agattcttca tacgcggcac
    29581 accgctatag ccgcaactgc catgcagttt atgaatcaaa tcaaccaggc cttccgggtt
    29641 ttctccaacc agctgttcct caactttgtt gcgaacttca ggcaggaaat cgagtaacat
    29701 ttgcagcata tcgcgcgcta aatcggtttt tcctgctgcc tggcgtagtg ccagttgcca
    29761 gtcgagggtc gcattcgggt tcaccacaat ttcgttgact tcgggcgtca cgacgcgaga
    29821 ggaaataccg ctgccaggct tgtagcgcaa caacaaatta tgcaatcgct cttcttcaat
    29881 cggtttcgcc agataatcgc tcatccctgc gccaagcagc ttctcttttt gcccggccat
    29941 tgcatgcgcc gttaccgcga taaccggcgt ttgttgctga tgggggagct ggtggatgag
    30001 ctcgcaggcc cgaatgccat ccatgtcagg catttgaata tccattaaga tcaaatcgaa
    30061 cggcatctgt ttcgcccgtt caaccgcctg atgcccgcta tcgcaaagtt ccacatgttg
    30121 caccatatct tccagcaatg cgccgataag tttcaggtta gcggggttgt catcaactgc
    30181 catgactgtc attgccagct tgctttcatc ggttacaggc aaaagcgtgt tttgtttgtg
    30241 atgacaaaat tccgtcaggg caggcaacag gcgcgtaggt gttaatggtt tcagcagaca
    30301 cgcgccgata ccatcttgct tgagtttttc tgcattgact tgtgcatggc aaggaagtgc
    30361 cagcatcagg aaatcggtca tcgataccgc tttcgctaat cgctcatgtt gcattgttag
    30421 cggctcgcgg aaggtcaccg cgatgcctaa caacatcatg tcgtaatgcg cgggaggcag
    30481 cgcggagaac gttgggctat aaaccacttc cagcggcgtt tcacttaaaa tatccagcgt
    30541 gcattgcgct gctgcggagt ttggttcgac ataggccagg cgcttaccgg cgaggcactg
    30601 ggtggatggc ccttcgataa taatgttcgg gttcagatcg agattaatgt ggaaccagaa
    30661 agttgaaccg cgattcggct ggctatggaa cgaaatatcg ccgcccattt cattaaccag
    30721 tttttgtgta atcaccagcc ccagaccggt gccaccatga cggcgggaaa tactggcatc
    30781 agcctgtcgg aaggcctgga ataagcgcga ttgatcgcgt tcaggaatac caatgccggt
    30841 atcccgaatc tgcacttcaa tctgcacttt ggtattactc agcgcacgtt tttctaccag
    30901 aatatcaatg ttgccattct cggtgaattt aattgcattc cccaccaggt tagtgatgat
    30961 ttgctgtaat cgcagtgggt cgccgatcac gttatcaggc acgtcgcttt taatattgag
    31021 cgtcagttcc aaccctttat cgtgagaaga atgtgccagc agagtaacga cttcatccag
    31081 cgtgctgcgt agtgggaatg gaatactttc cagaatcagc ttacctgctt ccagtttcga
    31141 gaagtcgaga acatcattaa taattgccag caaattattt gccgaacgtt caatcgtatt
    31201 caggtgatcg cgctgcgttg gtgttaattc tgttttcagc gtcaggcggg taaagccaat
    31261 aacaccattc agtggtgtac gcagctcgtg tgacatattt gccagaaact cggatttaat
    31321 acgcgccgct tcctgggcgc gctttttcgc cagatctaac tcaacgttct gaatttccat
    31381 ctgctccagc gtttcacgca gatcggacgt cgcctggtcg atattgtgct gcatctcttc
    31441 gtgataagca gccagcgaca ttgccatcga gttgataccg tttttcagca tatccagctc
    31501 gccgagcata aatccttcca cccggctgtc gagttgcccg cgacggatgc ggtcgacggt
    31561 attcaccatg ttgcgaatcg gaccggttac atcgcgcatt aagcgccagc caaaaataag
    31621 cgcaataccg atacaaaaca gcatcatcac gctggaaata aagatctctt tatattgctg
    31681 caagcgaacc gatttaagat ccagctccag cgcaatatat cccaacatat tttgactatt
    31741 tttggcatcg ctactgggcg attcgtcggg ggagtaactc tcagaaataa tcggcgtgcg
    31801 gaggatcata atatcgccgt cacgagtgac agtgagctgg cgaggaaacg gcacgttgct
    31861 gccgagctgc attgatgagg gatcaagatg aaaattggag gtgacaaaga gtcggttatt
    31921 ttcatcataa accgaaatcg cgcgaacaat atcggaatgg cgacgatgca gtacgcttat
    31981 taactgaccg atagattcgc gattttgcag gctcatgcca tattcagtag aaactgcaag
    32041 cggctcaata atgctggcac cggcatcttc cagttgacgc tgcaagtcgt tatagcgatg
    32101 cacgacgaaa aagatactca gcaataaacc aataaggacg gtcggtgcca ggatcagaat
    32161 catcatgcgt gcgcgcaggc tgtagttggt catggagttc cgttatggga caattaaggt
    32221 cacactgtta aattgagaaa gtctcgccaa tggcgcaatt ctactctgca aaacgacgca
    32281 cgacgacgcg tcagatcata accgtttcag tcaacgacct cgactctttt ggtcagggcg
    32341 tggcgcgaca taacggcaaa acgttattta tccccggatt attgccgcag gaaaacgcgg
    32401 aagttgctgt tactgaagat aaaaaacagt atgcccgcgc taaagtcgta cgccggttaa
    32461 gcgatagccc ggaacgcgaa acaccacgct gtcctcattt tggcgtatgc ggcggctgtc
    32521 agcaacaaca cgccagcgtg gatttacagc agcgaagcaa aagtgcggca ctcgcccgat
    32581 taatgaaaca cgatgtctct gaagtgatcg ccgatgttcc ctggggctat cgccgccgcg
    32641 cgcgtttaag tctgaactac ttaccgaaaa cacagcaact tcagatgggg tttcgcaaag
    32701 cgggctccag tgacattgtc gacgttaaac aatgccccat tttagtgccc caacttgaag
    32761 cattgctgcc caaagtcagg gcatgcctgg gcagcttaca agttatgcgc catcttggtc
    32821 atgttgaact ggtacaggca accagcggca cgctgatgat tttgcgccat accgcaccgc
    32881 taagttcggc agatcgcgaa aaactggaac gcttttcgca ttctgaaggc ctggatctgt
    32941 atctcgcccc cgatagtgag atactcgaaa ccgtctctgg tgagatgccc tggtatgact
    33001 caaacgggtt gcgcttaact tttagcccgc gcgattttat tcaggtcaat gcgggtgtga
    33061 accaaaaaat ggtagcgcgt gcgttggaat ggctggatgt gcaacctgaa gatcgcgtac
    33121 tggatctgtt ctgcggtatg ggcaacttta cactgccatt ggcgaaacaa gcagccagtg
    33181 tggtcggtgt agaaggtgtt ccggcgctgg tggaaaaagg ccagcagaat gcgcgtctta
    33241 atggtttaca gaatgtgacg ttttatcacg aaaatcttga agaagatgtc acaaagcagc
    33301 cgtgggcgaa aaacggcttc gataaagtgt tgctggaccc ggcgcgagca ggtgccgcag
    33361 gtgttatgca gcaaattata aaactggaac ctattcgtat agtttatgta tcctgtaacc
    33421 ctgcaacgct ggctcgggat agcgaagcgt tattaaaagc aggatatacc attgcgcgac
    33481 tggcgatgct ggatatgttc ccacacacgg gacatctgga atcgatggta cttttctcgc
    33541 gcgttaaata gttacgattt gccgatttcg gcaggtctgg tccctaaagg agaggacaat
    33601 ggttgcggta agaagtgcac atatcaataa ggctggtgaa tttgatccgg aaaaatggat
    33661 cgcaagtctg ggtattacca gccagaagtc gtgtgagtgc ttagccgaaa cctgggcgta
    33721 ttgtctgcaa cagacgcagg ggcatccgga tgccagtctg ttattgtggc gtggtgttga
    33781 gatggtggag atcctctcga cattaagtat ggacattgac acgctgcggg cggcgctgct
    33841 gttccctctg gctgatgcca acgtagtcag cgaagatgtg ctgcgtgaga gcgtcggtaa
    33901 gtcggtcgtt aaccttattc acggcgtgcg tgatatggcg gcgatccgcc agctgaaagc
    33961 gacgcacact gattctgttt cctccgaaca ggtcgataac gttcgccgga tgttattggc
    34021 gatggtcgat gattttcgct gcgtggtcat caaactggcg gagcgtattg ctcatctgcg
    34081 cgaagtaaaa gatgcgccgg aagatgaacg cgtactggcg gcaaaagagt gcaccaatat
    34141 ctacgcgccg ttggcgaacc gtcttgggat tgggcaactg aaatgggagc tggaagatta
    34201 ctgcttccgt tatctccacc cgaccgaata caaacgcatc gcaaaactgc tgcatgaacg
    34261 ccgtctcgac cgcgaacact atatcgaaga gtttgtcggc catctgcgcg ctgagatgaa
    34321 agctgaaggc gttaaagctg aagtgtatgg tcgtccgaaa cacatctaca gtatctggcg
    34381 caaaatgcag aaaaagaacc tcgccttcga tgagctgttt gatgtgcgtg cggtacgtat
    34441 tgtcgccgag cgtttacagg attgttatgc cgcactgggg atagtgcaca ctcactatcg
    34501 ccacctgccg gatgagtttg acgattatgt cgctaacccg aaaccaaacg gttatcagtc
    34561 tattcatacc gtggttctgg ggccaggtgg caaaaccgtt gagatccaaa tccgcaccaa
    34621 acagatgcat gaagatgcag agttgggtgt tgctgcgcac tggaaatata aagagggcgc
    34681 ggcagctggc ggcgcacgtt cgggacatga agaccggatt gcctggctgc gtaaactgat
    34741 tgcgtggcag gaagagatgg ctgattccgg cgaaatgctc gacgaagtac gcagccaggt
    34801 ctttgacgac cgggtgtatg tctttacgcc gaaaggtgat gtcgttgatt tgcctgcggg
    34861 atcaacgccg ctggacttcg cttaccacat ccacagtgat gtcggacacc gctgcatcgg
    34921 ggcaaaaatt ggcgggcgca ttgtgccgtt cacctaccag ctgcagatgg gcgaccagat
    34981 tgaaattatc acccagaaac agccgaaccc cagccgtgac tggttaaacc caaacctcgg
    35041 ttacgtcaca accagccgtg ggcgttcgaa aattcacgcc tggttccgta aacaggaccg
    35101 tgacaaaaac attctggctg ggcggcaaat ccttgacgac gagctggaac atctggggat
    35161 cagcctgaaa gaagcagaaa aacatctgct gccgcgttac aactttaatg atgtcgacga
    35221 gttgctggcg gcgattggtg gcggggatat ccgtctcaat cagatggtga acttcctgca
    35281 atcgcaattt aataagccga gtgccgaaga gcaggacgcc gccgcgctga agcaacttca
    35341 gcaaaaaagc tacacgccgc aaaaccgcag taaagataac ggtcgcgtgg tggtcgaagg
    35401 tgttggcaac ctgatgcacc acatcgcgcg ctgctgccag ccgattcctg gagatgagat
    35461 tgtcggcttc attacccagg ggcgcggtat ttcagtacac cgcgccgatt gcgaacaact
    35521 ggcggaactg cgctcccatg cgccagaacg cattgttgac gcggtatggg gtgagagcta
    35581 ctccgccgga tattcgctgg tggtccgcgt ggtggctaat gatcgtagtg ggttgttacg
    35641 tgatatcacg accattctcg ccaacgagaa ggtgaacgtg cttggcgttg ccagccgtag
    35701 cgacaccaaa cagcaactgg cgaccatcga catgaccatt gagatttaca acctgcaagt
    35761 gctggggcgc gtgctgggta aactcaacca ggtgccggat gttatcgacg cgcgtcggtt
    35821 gcacgggagt taggccgaaa tttgctcgta tctacaatgt agattgatat atactgtatc
    35881 tacatatgat agcggtttga ggaaagggtt atgatccaca gtagcgtaaa gcgttgggga
    35941 aattcaccgg cggtgcggat cccggctacg ttaatgcagg cgctcaatct gaatattgat
    36001 gatgaagtga agattgacct ggtggatggc aaattaatta ttgagccagt gcgtaaagag
    36061 cccgtattta cgcttgctga actggtcaac gacatcacgc cggaaaacct ccacgagaat
    36121 atcgactggg gagagccgaa agataaggaa gtctggtaat ggtaagccga tacgtacccg
    36181 atatgggcga tctgatttgg gttgattttg acccgacaaa aggtagcgag caagctggac
    36241 atcgtccagc tgttgtcctg agtcctttca tgtacaacaa caaaacaggt atgtgtctgt
    36301 gtgttcctta tacaacgcaa tcaaaaggat atccgttcga agttgtttta tccggtcagg
    36361 aacgtgatgg cgtagcgtta gctgatcagg taaaaagtat cgcctggcgg gcaagaggag
    36421 caacgaagaa aggaacagtt gccccagagg aattacaact cattaaagcc aaaattaacg
    36481 tactgattgg gtagtgttac taactcactt ctattctggt cacaggtttc cacctgacag
    36541 acccgcaatt ttcaggacaa ttcaatgaat caaatcgacc gtttgctcac tattatgcag
    36601 cgcctgcgcg atccggaaaa cggctgcccg tgggataaag agcagacatt tgccaccatt
    36661 gcgccttaca cccttgaaga aacctacgaa gtgctggacg ccatcgcccg tgaagatttt
    36721 gacgatcttc gcggtgaact gggcgatctg ctattccagg tggtgtttta cgcgcaaatg
    36781 gctcaggaag aagggcgctt tgactttaat gatatttgcg ctgctattag cgataaatta
    36841 gagcgtcgcc atccgcatgt ttttgctgat agttctgccg aaaacagtag tgaagtgctt
    36901 gcccgttggg agcaaatcaa aaccgaagag cgcgcgcaga aagcgcagca ttcggcgctg
    36961 gacgatattc ctcgtagttt accggcttta atgcgtgcgc aaaaaatcca gaaacgttgc
    37021 gccaacgttg gcttcgattg gacgacgctt ggtccggtag tcgataaagt ctacgaagag
    37081 atcgacgagg tgatgtacga agcgcggcag gctgttgtcg accaggctaa actggaggag
    37141 gaaatggggg acctgctgtt tgccacggtc aatctggctc gtcatttagg gacaaaagcg
    37201 gaaatcgcat tgcaaaaagc gaacgaaaaa tttgagcgtc gttttcgcga agtggagcgt
    37261 attgttgccg cgcgtggact ggaaatgaca ggtgttgacc tcgaaacaat ggaagaagtc
    37321 tggcaacagg taaaacggca ggaaattgat ctctaaggga attacgcggt caagcgccat
    37381 ttgtgtcatt ttttaaatga caagcgcttg atttgcgtca aaaacattta ccccaaaggg
    37441 gctattttct cactcctgat ttcaatagtg cgctggcgaa gaggagggat aatgaaagtt
    37501 tgtggcacag gtcatgttcg ggtatactgc tttcccgtcc tggttattcc atcgtctttt
    37561 caacctaact tctcaggttc agcatgacaa cgaactatat ttttgtgacc ggcggggtcg
    37621 tatcctctct gggtaaaggc attgccgcag cctccctcgc agccattctt gaagcccgtg
    37681 gcctcaatgt gaccatcatg aaactggatc cgtacatcaa cgtcgatcca ggtactatga
    37741 gcccaatcca acacggggaa gtgttcgtta ctgaagacgg cgctgaaacc gacctggacc
    37801 tggggcacta cgagcgtttc attcgtacca aaatgagccg ccgcaacaac ttcaccacgg
    37861 gtcgtatcta ctctgacgtt ctgcgtaaag aacgccgcgg tgactacctc ggcgcaaccg
    37921 tgcaggttat tccgcacatc actaacgcaa tcaaagagcg cgtgctggaa ggtggcgaag
    37981 gtcatgacgt agtactggta gaaatcggcg gtacagtagg tgatatcgaa tccttgccgt
    38041 tcctcgaagc gattcgccag atggctgttg aaattggccg tgagcacact ctgtttatgc
    38101 acctgacgct ggtgccgtac atggcagcgt ctggtgaagt caaaaccaaa ccgactcagc
    38161 actctgtaaa agagctgctc tccatcggta tccagcctga catcctgatt tgtcgttcag
    38221 atcgcgctgt tccggcgaac gaacgtgcga agattgcatt gttctgtaat gttccggaaa
    38281 aagcggttat ttctctgaaa gacgtcgatt ccatctataa aattccgggc ctgttgaaat
    38341 ctcaggggct ggacgattat atttgtaaac gattcagctt aaactgcccg gaagcgaatc
    38401 tgtccgaatg ggaacaggtt atcttcgaag aagcgaaccc ggtaagtgaa gtcaccatcg
    38461 gtatggtcgg caagtacatt gaactgccgg atgcttataa atcagtgatc gaagcactga
    38521 aacacggtgg gctgaagaat cgtgtcagcg tcaacatcaa actgatcgat tcacaagatg
    38581 ttgaaacgcg cggcgttgaa atccttaaag gtctggacgc aatcctcgta cctggcggtt
    38641 tcggctatcg tggcgtagaa ggcatgatta cgaccgcgcg ttttgcgcgt gagaacaata
    38701 ttccttatct gggcatttgc ctgggtatgc aggtggcgtt aattgattac gctcgccatg
    38761 ttgccaacat ggagaacgcc aactctacgg aatttgtgcc agactgtaag tacccggttg
    38821 tggcgctgat taccgagtgg cgcgatgaaa acggcaacgt tgaagttcgt agcgagaaga
    38881 gcgatctcgg cggtaccatg cgtctcggcg cacagcagtg ccagttggtt gacgatagcc
    38941 tggttcgcca gctgtacaat gcgccgacaa ttgttgagcg tcatcgtcac cgttacgaag
    39001 tcaacaacat gctgttgaaa cagattgaag atgcaggtct gcgcgttgcg ggccgttccg
    39061 gggatgatca gttggtcgag atcatcgaag ttccgaatca cccgtggttc gtggcttgcc
    39121 agttccatcc ggagtttact tctactccac gtgatggtca cccgctgttt gcaggctttg
    39181 tgaaagccgc cagcgagttc cagaaacgtc aggcgaagta agtaaaaaag ttagaacggc
    39241 aacgcgtacc ctgggtacgc gttgtttgtc tggagtttca gtttaactag tgacttgagg
    39301 aaaacctaat gtccaaaatc gtaaaaatca tcggtcgtga aatcatcgac tcccgtggta
    39361 acccgactgt tgaagccgaa gtacatctgg agggtggttt cgtcggtatg gcagctgctc
    39421 cgtcaggtgc ttctactggt tcccgtgaag ctctggaact gcgcgatggc gacaaatccc
    39481 gtttcctggg taaaggcgta accaaagctg ttgctgcggt aaacggcccg atcgctcagg
    39541 cgctgattgg caaagatgct aaagatcagg ctggcattga caagatcatg atcgacctgg
    39601 acggcaccga aaacaaatcc aaattcggcg cgaacgcaat cctggctgta tctctggcta
    39661 acgccaaagc tgctgcagct gctaaaggta tgccgctgta cgagcacatc gctgaactga
    39721 acggtactcc gggcaaatac tctatgccgg ttccgatgat gaacatcatc aacggtggtg
    39781 agcacgctga caacaacgtt gatatccagg aattcatgat tcagccggtt ggcgcgaaaa
    39841 ctgtgaaaga agccatccgc atgggttctg aagttttcca tcacctggca aaagttctga
    39901 aagcgaaagg catgaacact gcagttggtg acgaaggtgg ctatgcgccg aacctgggtt
    39961 ccaacgctga agctctggct gttatcgctg aagctgttaa agctgctggt tatgaactgg
    40021 gcaaagacat cactctggcg atggactgcg cagcttctga attctacaaa gacggtaaat
    40081 acgttctggc tggcgaaggc aacaaagcgt tcacctctga agaattcact cacttcctgg
    40141 aagaactgac caaacagtac ccgatcgttt ctatcgaaga cggtctggac gaatctgact
    40201 gggacggttt cgcataccag accaaagttc tgggcgacaa aatccagctg gttggtgacg
    40261 acctgttcgt aaccaacacc aagatcctga aagaaggtat cgaaaaaggt atcgctaact
    40321 ccatcctgat caaattcaac cagatcggtt ctctgaccga aactctggct gcaatcaaga
    40381 tggcgaaaga tgctggctac actgcagtta tctctcaccg ttctggcgaa actgaagacg
    40441 ctaccatcgc tgacctggct gttggtactg ctgcaggcca gatcaaaact ggttctatga
    40501 gccgttctga ccgtgttgct aaatacaacc agctgattcg tatcgaagaa gctctgggcg
    40561 aaaaagcacc gtacaacggt cgtaaagaga tcaaaggcca ggcataagac tgactttatc
    40621 tgatttaaaa atgccagcct ccgggctggc attttttatg gttgagttac cagcggccgg
    40681 atgcgccgcc acctccagaa gaaccgccac cgccgctaaa tccgccgcca cttgagcctc
    40741 caccggaacc gctgttgtct gaatgataac ttttacctga tgctcttttc tgaagtacac
    40801 ataaacaggt aaagaccata aatatggtga aagtaaaaaa gaaggaaaat acagcaatgg
    40861 aaaaaatttt atcggagaac aggaataaat ctaaaagata gattgctgaa atatagactc
    40921 cacttttaca tgcgcgacaa aaattactgc cttgatgaaa aaaccagaaa gggaagaaca
    40981 tcactgcaaa tacccaaaaa atagcaaagt aataatgatc actggaagac gctgattcac
    41041 tttctgaagg attagtcgga tattggtgtt gtgaagtgag ttggttattc aaagcgttta
    41101 tagctagctc taatccctga gcaaattttt gttgtttaaa tgcgggtatc atgttgctac
    41161 ggatgatatc tccagccaga gcatcggtta ccttttcttc cagcccataa cctacctgga
    41221 tgcggacagt acgatctgac caggcaacaa tgatcagtat cccatcatta cgtttggcat
    41281 ctcctaaacg ccaattgtca aaaactcttg tagcatattg ttcaattgtc tcgtctttgg
    41341 ttgtgggaac aattaataca gcaatttgca cttgggtatt attgaaaata gattctaact
    41401 tgtgcgtcag ttctttcttt tcttgtgagt ttaagctact cgtaagatcc gtaacttgtt
    41461 gctgtaattg tgggacaata gttggttggg ctgcaacaaa
//
LOCUS       DAZGJW010000025        40327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZGJW010000025 DAZGJW010000000
VERSION     DAZGJW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.025"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..130
                     /locus_tag="ACHKIK_001390"
     CDS             <1..130
                     /locus_tag="ACHKIK_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO9082597.1"
                     /translation="YYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA"
     gene            109..213
                     /gene="speFL"
                     /locus_tag="ACHKIK_001391"
     CDS             109..213
                     /gene="speFL"
                     /locus_tag="ACHKIK_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO9082598.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            524..2722
                     /gene="speF"
                     /locus_tag="ACHKIK_001392"
     CDS             524..2722
                     /gene="speF"
                     /locus_tag="ACHKIK_001392"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO9082599.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2719..4038
                     /gene="potE"
                     /locus_tag="ACHKIK_001393"
     CDS             2719..4038
                     /gene="potE"
                     /locus_tag="ACHKIK_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO9082600.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4094..5734)
                     /gene="pgm"
                     /locus_tag="ACHKIK_001394"
     CDS             complement(4094..5734)
                     /gene="pgm"
                     /locus_tag="ACHKIK_001394"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO9082601.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGT
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNALGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5760..6305)
                     /gene="seqA"
                     /locus_tag="ACHKIK_001395"
     CDS             complement(5760..6305)
                     /gene="seqA"
                     /locus_tag="ACHKIK_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO9082602.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSASSQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVRGTI"
     gene            6490..7254
                     /gene="ybfF"
                     /locus_tag="ACHKIK_001396"
     CDS             6490..7254
                     /gene="ybfF"
                     /locus_tag="ACHKIK_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO9082603.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7394..7687
                     /gene="ybfE"
                     /locus_tag="ACHKIK_001397"
     CDS             7394..7687
                     /gene="ybfE"
                     /locus_tag="ACHKIK_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO9082604.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7827..8357
                     /gene="fldA"
                     /locus_tag="ACHKIK_001398"
     CDS             7827..8357
                     /gene="fldA"
                     /locus_tag="ACHKIK_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO9082605.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8567..8653
                     /gene="uof"
                     /locus_tag="ACHKIK_001399"
     CDS             8567..8653
                     /gene="uof"
                     /locus_tag="ACHKIK_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO9082606.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8646..9092
                     /gene="fur"
                     /locus_tag="ACHKIK_001400"
     CDS             8646..9092
                     /gene="fur"
                     /locus_tag="ACHKIK_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO9082607.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9176..9502)
                     /gene="chiQ"
                     /locus_tag="ACHKIK_001401"
     CDS             complement(9176..9502)
                     /gene="chiQ"
                     /locus_tag="ACHKIK_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO9082608.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9552..10958)
                     /gene="chiP"
                     /locus_tag="ACHKIK_001402"
     CDS             complement(9552..10958)
                     /gene="chiP"
                     /locus_tag="ACHKIK_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO9082609.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11404..13068)
                     /gene="glnS"
                     /locus_tag="ACHKIK_001403"
     CDS             complement(11404..13068)
                     /gene="glnS"
                     /locus_tag="ACHKIK_001403"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO9082610.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13271..15217)
                     /gene="nagE"
                     /locus_tag="ACHKIK_001404"
     CDS             complement(13271..15217)
                     /gene="nagE"
                     /locus_tag="ACHKIK_001404"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO9082611.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            15550..16350
                     /gene="nagB"
                     /locus_tag="ACHKIK_001405"
     CDS             15550..16350
                     /gene="nagB"
                     /locus_tag="ACHKIK_001405"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO9082612.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16410..17558
                     /gene="nagA"
                     /locus_tag="ACHKIK_001406"
     CDS             16410..17558
                     /gene="nagA"
                     /locus_tag="ACHKIK_001406"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO9082613.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            17567..18787
                     /gene="nagC"
                     /locus_tag="ACHKIK_001407"
                     /gene_synonym="nagR"
     CDS             17567..18787
                     /gene="nagC"
                     /locus_tag="ACHKIK_001407"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO9082614.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18835..19587
                     /gene="nagD"
                     /locus_tag="ACHKIK_001408"
     CDS             18835..19587
                     /gene="nagD"
                     /locus_tag="ACHKIK_001408"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO9082615.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            19844..21508
                     /gene="asnB"
                     /locus_tag="ACHKIK_001409"
     CDS             19844..21508
                     /gene="asnB"
                     /locus_tag="ACHKIK_001409"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO9082616.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21889..21965
                     /locus_tag="ACHKIK_001410"
     tRNA            21889..21965
                     /locus_tag="ACHKIK_001410"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21923..21925,aa:Met,seq:cat)
     gene            21974..22058
                     /locus_tag="ACHKIK_001411"
     tRNA            21974..22058
                     /locus_tag="ACHKIK_001411"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22008..22010,aa:Leu,seq:tag)
     gene            22082..22156
                     /locus_tag="ACHKIK_001412"
     tRNA            22082..22156
                     /locus_tag="ACHKIK_001412"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22114..22116,aa:Gln,seq:ttg)
     gene            22191..22265
                     /locus_tag="ACHKIK_001413"
     tRNA            22191..22265
                     /locus_tag="ACHKIK_001413"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22223..22225,aa:Gln,seq:ttg)
     gene            22281..22357
                     /locus_tag="ACHKIK_001414"
     tRNA            22281..22357
                     /locus_tag="ACHKIK_001414"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22315..22317,aa:Met,seq:cat)
     gene            22406..22480
                     /locus_tag="ACHKIK_001415"
     tRNA            22406..22480
                     /locus_tag="ACHKIK_001415"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22438..22440,aa:Gln,seq:ctg)
     gene            22518..22592
                     /locus_tag="ACHKIK_001416"
     tRNA            22518..22592
                     /locus_tag="ACHKIK_001416"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22550..22552,aa:Gln,seq:ctg)
     gene            complement(22746..23921)
                     /gene="ubiF"
                     /locus_tag="ACHKIK_001417"
     CDS             complement(22746..23921)
                     /gene="ubiF"
                     /locus_tag="ACHKIK_001417"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO9082617.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGGNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            24067..25491
                     /gene="miaB"
                     /locus_tag="ACHKIK_001418"
     CDS             24067..25491
                     /gene="miaB"
                     /locus_tag="ACHKIK_001418"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO9082618.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            25644..26684
                     /gene="ybeZ"
                     /locus_tag="ACHKIK_001419"
     CDS             25644..26684
                     /gene="ybeZ"
                     /locus_tag="ACHKIK_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO9082619.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            26681..27148
                     /gene="ybeY"
                     /locus_tag="ACHKIK_001420"
     CDS             26681..27148
                     /gene="ybeY"
                     /locus_tag="ACHKIK_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO9082620.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            27238..28116
                     /gene="corC"
                     /locus_tag="ACHKIK_001421"
     CDS             27238..28116
                     /gene="corC"
                     /locus_tag="ACHKIK_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO9082621.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            28141..29679
                     /gene="lnt"
                     /locus_tag="ACHKIK_001422"
     CDS             28141..29679
                     /gene="lnt"
                     /locus_tag="ACHKIK_001422"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO9082622.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIVSLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGTGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLLGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(29757..31634)
                     /locus_tag="ACHKIK_001423"
     CDS             complement(29757..31634)
                     /locus_tag="ACHKIK_001423"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO9082623.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSFILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            32089..32997
                     /gene="gltI"
                     /locus_tag="ACHKIK_001424"
     CDS             32089..32997
                     /gene="gltI"
                     /locus_tag="ACHKIK_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO9082624.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            33167..33907
                     /gene="gltJ"
                     /locus_tag="ACHKIK_001425"
     CDS             33167..33907
                     /gene="gltJ"
                     /locus_tag="ACHKIK_001425"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO9082625.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            33907..34581
                     /gene="gltK"
                     /locus_tag="ACHKIK_001426"
     CDS             33907..34581
                     /gene="gltK"
                     /locus_tag="ACHKIK_001426"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO9082626.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            34581..35306
                     /gene="gltL"
                     /locus_tag="ACHKIK_001427"
     CDS             34581..35306
                     /gene="gltL"
                     /locus_tag="ACHKIK_001427"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO9082627.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            35424..36359
                     /gene="rihA"
                     /locus_tag="ACHKIK_001428"
     CDS             35424..36359
                     /gene="rihA"
                     /locus_tag="ACHKIK_001428"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO9082628.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            36443..38113
                     /gene="hscC"
                     /locus_tag="ACHKIK_001429"
     CDS             36443..38113
                     /gene="hscC"
                     /locus_tag="ACHKIK_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO9082629.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELGALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQNSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(38173..39624)
                     /gene="djlC"
                     /locus_tag="ACHKIK_001430"
     CDS             complement(38173..39624)
                     /gene="djlC"
                     /locus_tag="ACHKIK_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFO9082630.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(39621..>40327)
                     /gene="ybeU"
                     /locus_tag="ACHKIK_001431"
     CDS             complement(39621..>40327)
                     /gene="ybeU"
                     /locus_tag="ACHKIK_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO9082631.1"
                     /translation="NKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSSW
                     GINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFVA
                     QTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSCVYVRAHHYYHSWMHY
                     FSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPL
                     TVQPECPVTLKDVSDL"
BASE COUNT         9869 a        10316 c        10162 g         9980 t
ORIGIN      
        1 ttattatttg gggtatgatc gttttttgtt gatcttcttc acagattata gccatttcat
       61 ggatagaata actctacctt caactgacac agcaagaggt aaaggtaaat ggaaaataat
      121 aaccgcttaa tgcctcatat aaggcggaca acccatatca tgaagtttgc ccatcgtaat
      181 agttttgact ttcatttctt taatgcccgc tagtcttctg actaaagggc accccaacgt
      241 acaggtctcc ctgactttaa gcattacagg ttaatacctg tattcctcgg tgctcatata
      301 ctgctaaccc ttttaaactc taaataattc gagtcgcagc acttgcaact tgaggtatga
      361 cgagtatagc cagttaccgg gctggtctgg gttattgcat ctgcaaaaag caaactactg
      421 atttatttat cagcggtgga gctttgcttt ttttccggcg tgatcgattt ctcctttgag
      481 aaattgagga cctgctatta cctgaaataa agagatgaac aaaatgtcag aattaaaaat
      541 tgccgttagt cgttcttgcc cggattgttt ttccactcat cgtgcatgcg tgaatataga
      601 cgaaagtaat tatattgacg ttgccgccat tattttatca gtcagtgatg ttgaacgtgg
      661 aaaactcgat gaaatagacg ctactggcta tgacattcct gtttttattg caacggaaaa
      721 tgaagaacgt gtacccgcag aatatctgcc acgtatttct ggtgtcttcg aacattgcga
      781 atcacgtaaa gagttttatg gacgtcagtt agaaaccgct gccagccatt atgaaacgca
      841 actgcgcccg cccttcttcc gcgcactggt cgattatgtt aatcagggca atagtgcttt
      901 tgactgccct ggtcatcagg ggggtgagtt tttccgtcgc catccggcag gcaatcagtt
      961 cgtggaatac tttggcgaaa tgctcttccg ctctgacttg tgtaatgctg acgtagcaat
     1021 gggcgatctg ctgattcatg aaggtgcgcc atgtattgca caacagcatg cggctaaagt
     1081 ctttaatgcc gataaaacct atttcgtttt aaacggtact tcatcttcta ataaagtggt
     1141 cttaaacgca ttgctgacgc cgggcgatct ggtgttgttt gaccgcaata accataaatc
     1201 taaccaccac ggtgcgttgc tacaggctgg cgcgacaccg gtttatctgg aaaccgcacg
     1261 taacccgtat ggctttatcg gtggcattga tgcgcactgt tttgaagaaa gttacctgcg
     1321 tgagctgatc gcggaagtcg caccgcagcg ggcaaaagag gctcgtcctt tccgcctcgc
     1381 tgtgattcag ttaggcacct acgacggtac gatttataac gcccgccagg tggtggataa
     1441 aattggtcat ctgtgtgact acattctgtt tgactccgca tgggttggct atgagcaatt
     1501 tatcccgatg atggctgact gttcgccgct gttgctggat cttaatgaga acgatccggg
     1561 tattctggtt acgcaatccg tacataaaca gcaggctggt ttttctcaaa cttcacaaat
     1621 ccataaaaaa gacagccaca tcaaagggca acagcgttat gtaccgcaca aacgcatgaa
     1681 caacgccttt atgatgcatg cctccaccag cccgttctat ccgctgtttg ccgcactgga
     1741 tatcaacgcc aaaatgcatg aaggtgtcag cggtcgtaat atgtggatgg attgtgtggt
     1801 aaatggcatt aatgcccgca agctgatcct cgataactgt cagcatattc gtccgttcgt
     1861 acctgaactg gtggatggta aaccctggca gtcgtatgaa acagcgcaaa ttgcggttga
     1921 tctgcgcttc ttccagtttg taccaggtga gcactggcac tcttttgaag gctatgcaga
     1981 gaatcaatac tttgtcgatc catgcaaact gttgctgaca accccaggta ttgatgcacg
     2041 taacggcgaa tatgaagcgt tcggtgtacc cgcgacgatt cttgctaact tcctgcgcga
     2101 aaatggcgta gtgccggaaa aatgcgatct taactccatc ctcttcctgc tgactccggc
     2161 agaagatatg gccaagcttc agcaacttgt tgccctgctg gtacgcttcg aaaaactgct
     2221 tgagtccgac gcgccattag cagaagtgct gccttccatc tacaaacagc atgaagagcg
     2281 ctacgccggt tataccctgc gtcagttgtg tcaagaaatg catgatttgt atgcccgtca
     2341 caacgtgaaa caactgcaaa aagagatgtt ccgtaaggaa cacttcccac gcgtaagcat
     2401 gaatccgcaa gaagccaact acgcctattt acgcggtgaa gtggagctgg ttcgtctgcc
     2461 ggatgcagaa ggccgtatcg ctgccgaagg tgcgcttcct tatcctccgg gtgtgctgtg
     2521 tgttgttccg ggtgaaatct ggggtggtgc tgttttgcgt tacttcagcg ctctggaaga
     2581 agggatcaac ctgctgccag gttttgcacc ggagctgcag ggtgtctata tcgaagaaca
     2641 tgatggtcgt aagcaagttt ggtgctatgt catcaagcct cgtgatgcgc aaagcaccct
     2701 gttgaaaggg gaaaaattat gagtcaggct aaatcgaaca aaatgggcgt cgttcagtta
     2761 accatactga cgatggtcaa catgatgggc tccggtatca tcatgctgcc gacaaagctt
     2821 gccgaagttg ggacaatttc aattatctcc tggctggtga cagccgtagg ctcaatggca
     2881 ctggcatggg cattcgcaaa atgcggtatg ttcagccgta aatcaggcgg tatgggcggt
     2941 tatgcggagt atgctttcgg taaatccggt aactttatgg cgaactatac ctacggcgtc
     3001 tcgctgctga ttgctaacgt cgcgattgct atttcggcgg ttggttacgg caccgaactg
     3061 ctcggcgcga gtttgtcgcc agtgcagatt ggtcttgcga ccatcggggt gctgtggatt
     3121 tgtaccgtgg ctaactttgg tggtgcgcgc attaccgggc aaatcagtag cattaccgtg
     3181 tggggggtca ttattccggt ggtaggcctg tgcattatcg gctggttctg gtttagcccg
     3241 acgctttacg ttgattcctg gaatccgcat catgcaccgt tcttcagtgc agtaggttct
     3301 tccatcgcta tgacgctgtg ggcttttctc ggtctggagt ctgcgtgtgc gaatactgat
     3361 gtagtggaaa acccggaacg taatgtgcca atcgcggtac tcggcggtac gttaggtgcg
     3421 gcggtgattt atatcgtctc caccaacgtg attgcaggga ttgtgcctaa tatggagctg
     3481 gcaaattcaa cggcaccatt tggcctggcc ttcgcacaga tgttcacacc agaagtgggt
     3541 aaagtcatta tggcgctgat ggtgatgtcc tgctgcggtt cgctgcttgg ctggcagttc
     3601 accattgccc aggtgtttaa atcttcatct gatgaaggct acttccctaa aattttctcc
     3661 cgtgtaacca aagtggatgc accggtgcag ggaatgttga ccattgtgat tatccagagc
     3721 gggttggcgc tgatgacgat tagcccgtcg ctaaacagtc agttcaacgt gctggttaac
     3781 ctggccgtgg tgaccaatat cattccgtat attctgtcaa tggcggcatt agtcattatt
     3841 cagaaggtcg ctaatgtgcc cccctcaaaa gcgaaagttg caaactttgt tgcttttgtt
     3901 ggcgcgatgt atagctttta tgcgctgtac tcatccgggg aagaagccat gctgtacggt
     3961 tccatcgtga ccttccttgg ttggacactg tatggtctgg tatcaccacg ctttgaactg
     4021 aaaaataaac acggttaaaa aagcaaagta aaaaagggcg atcttgcgac cgcccttttt
     4081 ttattaaatg tgtttacgcg tttttcagaa cttcgctaac aatctcaacc gcttctttct
     4141 caatctgctt gcgatgttct tcaccgagga agctttcgca gtagatctta tatgcgtctt
     4201 ccgtgcctga cggacgcgcg gcgaaccagc cgttgtcagt catcactttc agaccgccga
     4261 tagaagcacc attacccggt gccgccgtca gacgtgcggt gatcgggtca cctgccaggg
     4321 tgctggcgct caccatttcc ggagacagct tagacaacgc cgctttttgt gcggaagtcg
     4381 cagcggcctg caaacggttg tagctcggcg caccaaagcg ttttgccagt tcgttgtagt
     4441 gttcctgcgg gttcttaccg gtgacagcgg tgatttccgc cgccagcaga cacataatga
     4501 tgccgtcttt atcggtggac cacggcgtgc cgtcgaaacg caggaaggaa gccccggcgc
     4561 tctcttcgcc gccaaagccg aaactgccgt cgaacagacc atcaacaaac catttgaatc
     4621 ctaccgggac ttctaccagt ttacggccca aggcgttgac cacacggtcg atcatcgcag
     4681 aagaaaccag cgttttaccg acagcaacat ctttgcccca ctgcggacga tgctggaaca
     4741 ggtagttgat cgccaccgcc aggtagtggt tcggattcat caaacctgcc ggagtgacga
     4801 taccgtgacg gtcataatcc gggtcgttag caaacgccag atcgaactta tcacgcagtg
     4861 ccagcaggcc cgccatcgca cactcggagg agcagtccat acggatcgtg ccgtctttat
     4921 caaggtgcat aaagcggaag gtttgatcga cctgatcgtt aacgatggtc aggttgaggt
     4981 tgtaatactc gccaatacgc ttccagtatt cgataccgga accgcccagc ggatcaacgc
     5041 caagcgtcag gcccgctttc tgaatcgccg ccatatcaac gatatcggcc agaccttcca
     5101 caaacggctg caccagatcc tgctctttca catgaccgga tgccatcgct tcgtcgagag
     5161 agatacgctt cacgcctttc aggccatcgg ccatcagtgc gttggccctg tcttccacca
     5221 ctttggtgac gttggtatca gccgggccac catttggcgg gttgtacttg ataccaccat
     5281 cttccggcgg gttatgggac ggtgtaatca cgataccgtc tgccagcggg ccaccttttt
     5341 tattgtgaac caggatggca ttggaaacgg caggcgtcgg agtgaatcca ttgttttcct
     5401 gcacaatgac atcaacgccg ttcgctgcca gcacttccag tacggaaatg aacgcaggct
     5461 cggacagggc gtgagtatct ttacccacat agcaagggcc agtgatgcca tttttcgcac
     5521 gttcttcagc aattgcctga gcgatcgcca gaatgtgcgg ctcgttaaag ctgtggcgcg
     5581 ctgcactgcc acggtgaccg gaagtaccga atttcaccgc gtgctccgca ttccctgctt
     5641 ctggtttcag tacataatat tgcgccgtca gttgggcgac gttaatcaaa tcactctgtt
     5701 gtgcaggttg gcctgcacga ttgtggattg ccattgcttt gtcctttgtc tgcaacgttt
     5761 tagatagttc cgcgaacctt ctcaatcaat tccgccggga attgcatcga ctgcatgatg
     5821 tgttcgatca tgctgcattt acggccggtg ttggtgttgg tgatcaccca atacggcgtg
     5881 cctggcacat gtttcggctt ggtctgatta ccatttttca gcagcgtttg ttcatctgcc
     5941 gcaaagtaaa cgcgtgtacg accgtgcaac gattccgttg cttcggcaaa cgcctgggcg
     6001 tcaagagaat atagtgtaga caacagcagc ataaagcgat tgaccgctcg cttttgctct
     6061 gcgtattcgt ccgaaagcag aagttcacgc attgcgcgaa ccttgtcttt aatcgttttg
     6121 accggcttcg cttcgacgat agcaggtgac gcaacgcgaa cctctttcgt caccggagca
     6181 gcaggctgtg atgaggcgga aaatttcaac atacgccgta aaatgtcgga tgcgctctcg
     6241 ccgatatgct tagtgtggct ggcaatatag ctgtagagtt catcatcaac ttcaatcgtt
     6301 ttcatcttaa tccagtgcag tgtcttagct gaatacaagt cgccaggagt atatgaataa
     6361 atcccggcag cggatagcgt caagcctggc ttgcagaaaa actcggaata aaccttattt
     6421 gttgcagccc tgtggcacaa tggtcaacat aataccctaa cccgacaacg cgaaaaaaag
     6481 aactttgcca tgaaattgaa tatccgcgcg caaactgcac aaaaccagca caataattct
     6541 cccatcgttc ttgtccatgg tctgtttggt agcctcgaca accttggcgt actggctcgc
     6601 gatctggtaa acgatcacaa tatcatccag gttgatatgc gtaaccacgg tctttcaccg
     6661 agagatccgg taatgaatta cccggcgatg gcgcaggatc ttgttgatac tctggatgca
     6721 cagcagatcg acaaagcaac atttatcggt cactccatgg gcggtaaagc ggtaatggca
     6781 cttactgcac tggcccccga tcgcatcgat aaactggtgg cgatcgatat cgcaccggtc
     6841 gactatcacg tacgccgtca tgatgagatt ttcgcggcta tcaacgcggt cagtgaatcg
     6901 gacgcacaaa ctcgccagca agcagcagca ataatgcgcc agcatcttaa tgaagaaggg
     6961 gtgattcagt ttctgttgaa atcttttgtt gacggggagt ggcgctttaa cgtgccggta
     7021 ttgtgggatc agtatccgca tattgtgggt tgggagaaaa tcccggcatg ggatcaccct
     7081 gccctgttta tccctggcgg caattctccg tatgttagcg agcagtaccg tgatgattta
     7141 ctggcacaat ttccacaggc acgggcgcat gtgattgcag gcgcgggtca ctgggtccat
     7201 gctgaaaaac cggatgcggt attacgcgct atccgtcgct atctcaatga ttaacgcaac
     7261 tgattaaaaa cccagcgtcc gcgtcgcttt ccgcgcgtgg gcgttggcgt gactcatcgc
     7321 gctgatgtat tatggcgcgc tatccatccg ggcagaggcc tggctgattg tttcccccga
     7381 agtcaccaag atcatggcca aagaacaaac ggaccgtacg acattagatc tgttcgcgca
     7441 cgagcgtcga ccgggacgac cgaaaactaa tccgctttcg cgcgatgaac agctgcgtat
     7501 taataaacgc aaccagctaa aacgcgacaa agtacgtggc cttaagcgtg tcgaactgaa
     7561 gctgaacgcg gaagctgtcg aggcgctgaa cgagctggcg gagtcgcgca atatgagccg
     7621 tagcgaactg atcgaagaga tgttaatgca gcaactggcg gcgctgcgta gtcagggcat
     7681 cgtttaaatt tccactttca tgtagcacag tgtgcagtcc tgctcgtttg ctgattccgc
     7741 aagactgcct gttctgctat gattgccttt atccgtgggc aattttccac ccccatttca
     7801 ataagtttca agaggttatt tcactcatgg ctatcactgg catctttttc ggcagcgaca
     7861 ccggtaatac cgaaaatatc gcaaaaatga ttcaaaaaca gcttggtaaa gacgttgccg
     7921 atgtccatga cattgcaaaa agcagcaaag aagatctgga agcttatgac attctgctgc
     7981 tgggcatccc aacctggtat tacggcgaag cgcagtgtga ctgggatgac ttcttcccga
     8041 ctctcgaaga gattgatttc aacggcaaac tggttgcgct gtttggttgt ggtgaccagg
     8101 aagattacgc cgaatatttc tgcgacgcat tgggcaccat ccgcgacatc attgaaccgc
     8161 gcggtgcaac catcgttggt cactggccaa ctgcgggcta tcatttcgaa gcatcaaaag
     8221 gtctggcaga tgacgaccac tttgtcggtc tggctatcga cgaagaccgt cagccggaac
     8281 tgaccgctga acgtgtagaa aaatgggtta aacagatttc tgaagagttg catctcgacg
     8341 aaattcttaa tgcctgatgt gatgcggcgt agactcatgt ctacgccgta ttaatagata
     8401 atgccaatca aaataattgc tacaaatttg taacttttgc tgttgtacct gtacaatgtc
     8461 ccggtgttca agtggccttg ccgttgtaaa tgtaagctgt gccacgtttt tattaacaat
     8521 atttgccagg gacttgtggt tttcatttag gcgtggcaat tctataatga tacgcattat
     8581 ctcaagagca aattctgtca cttcttctaa tgaagtgaac cgcttagtaa caggacagat
     8641 tccgcatgac tgataacaat accgccctaa agaaagctgg cctgaaagta acgcttccac
     8701 gtttaaaaat cctggaagtt cttcaggagc cggacaacca tcacgtcagt gcggaagatt
     8761 tatacaaacg tctgatcgat atgggtgaag aaattggtct ggctacggta tatcgcgtac
     8821 tgaaccagtt tgacgacgct ggtatcgtca cccgccacaa ttttgaaggc ggtaaatccg
     8881 tatttgaact gacacagcaa catcaccacg atcacctgat ttgcctcgac tgcggcaagg
     8941 ttatcgaatt tagtgatgat tccatcgaag cgcgtcagcg tgaaattgcc gcaaaacatg
     9001 gcattcgcct gactaaccac agtctctatc tttacggtca ctgtgccgaa ggcgattgcc
     9061 gcgaagatga gcacgcgcac gaaggcaaat aagccagcct gaacgagaaa agccaacctg
     9121 cgggttggct tttttataca agggaaataa gaaaagcgag agttacagct ctcacttatt
     9181 tgttattact gcgtatttcc tgccagacct tatcacaatc ggctttcacc gcctgatcat
     9241 ttccggtttg cgttgcacgc agacactgct gataatccag tacgcgtaca ctttcctggt
     9301 cggcaaactg ctgatgttgt ttctctttct tcagcacgtt taacacgctc tggcaggctt
     9361 caattttttc tggcgaacct tgtgcggtgt tgatacaggc gctgtatgcc tctttcagac
     9421 gactgtcttc ctgtggcgcg gttgattgcg cacaagctac cagccccgat gccattatgg
     9481 cgatgagaat cagttttttc atagcaaatt cctcaaacgt gcggcaggca caaaacctgc
     9541 cgcgtcgggc atcagaagat ggtgaatggt gcgattacca taaatttcac gtcacgctca
     9601 tcctggaaga tgttaccgta accgccgccc cagcttggga tgtcagagtg gttgtcgtat
     9661 tcggtgaagt gcagtttgaa catcgtgcct ttggcgcgac cgtcctgaat ggtgtagacc
     9721 gcatccaggc tgtaggcaga ctcttcaata gtccggtttt tgtcgtagta cgcatccgga
     9781 ttgctctgcc aggtcgcagg tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg
     9841 aagcctggaa gattccagtt tttcaggtca tacatcgcac cgaagaagac cgctttttcg
     9901 ccgttggcgt tgaagtcgga acggttatcc caccagatat ccaggcggcc gttggaggaa
     9961 gcgtaggttg gagtcatacg ttgcaggaag tatccctgct gaccgtcagc cttaacccag
    10021 gtgccttcga ggcgcaaatc cactacgtca gccgcccggt aaccaaaggt caacgcttgc
    10081 agccaggcgg tgccgtcata aaggtcgttg acgctgcgat cgtcaacttt atcgcgcgta
    10141 ccatagaact ggtagctggt ggttaacggg ctaccggcga tatcaaattt gtagctggct
    10201 ttggcaaaat attgatcgat atacccttcc gcctgaccaa atgccgcttc cagtacgaag
    10261 ttatttttga agtcgtattt cgcaccgatg gagtgcaggt aatcaacttt ggtggtttta
    10321 tcgttctgat aaaactcatc catttccaga tgccacggcg ctttgtattc gttggtccac
    10381 atgtaggaga aactcaaggc accagcatcg ccgtaatcaa aattcgcccc ggcttccgcc
    10441 ccctgataag tacctggcat aaagctccag tgcggcgcta acagcgtttg accagttggc
    10501 tgaatgtaac ctgccctcgc ccaaaccgga ccgtatttaa atttggccgc agctttatac
    10561 aggcttatac cgcttttgtc gccggaccag tcttcgtcat aggctttatt actttttgaa
    10621 aacgcgattt cgttcgggtg ggagctgtcg ccgttttccg ccatttcaat cgccgtaaac
    10681 gcagcaatat caaggccgaa catatcagca gcataaccgg actgaaaatc gaggttggca
    10741 ttccaggtgg agtgagaaag gttggttttg tatttgtcgc cgtcggtaac atctttacgg
    10801 tcgcgttcac gctgccagta atatataccg ccggttaagg ttgaatcgtc gatgaatcct
    10861 tcggcgcgag cctccggcat tgccataaag cccgacattg ctgtaacacc ggcgatagcc
    10921 agcgccagcg tactacgttt gccactaaac gtacgcatgg gttaatcctc tttgacgtat
    10981 aaattgctgc accaaaggtg aacagcgaaa aaataaaaat taaaaaagta gcgttgagaa
    11041 aagtctcgct gactacagga acagactatt tttcgcgacg cgaattatca atctctttct
    11101 gtaaccagaa tgtaagaaca tgagcaaccg cacatatttt gatgattttt gttacaaggc
    11161 ttaagtttgc ggttgaaagg cttgcagaag aataaaagcg tgaatttcca gcaagatgaa
    11221 agcggttaca ttttatttaa ttatggtttg tgattttaaa tttattttat ggataaattg
    11281 cgaagcggaa ttaattcgcg aagaaaagca gaaaaaacgc cgcagagtgc ggcgtttggg
    11341 attgtttgct atttcttgcc ggatgcgacg taaatgcctc acccggcctg gtagaactta
    11401 aaattactcg cctactttcg cccaggtatc acgcagccca acggtgcggt taaataccgg
    11461 tttttccgct gtggaatggc ggctgtcgag gcagaagtaa ccttcacgct caaactggaa
    11521 tgctttaccc gcaaccgcat ctttcagcga cggttcagca aagccctgtt tgatcaccag
    11581 cgattccggg ttaatcaccg acaggaaatc atccgcagca ccagggttcg gcacgctgaa
    11641 cagacggtcg tacagacgga tttcaaccgg cagcgcatgt gccgcgctca cccagtgaat
    11701 aacacctttg actttacgac catctgccgg atctttgctt aaggtgtcgg cgtcataagt
    11761 acagaagatg gtggtgatat tgccttcggc atctttctcc acgcgttcag ccttaatgac
    11821 gtaagcatta cgcagacgca cttctttacc cagcaccaga cgtttgtact gcttgttagc
    11881 ttcttcgcgg aaatcggcgc gatcgatcca aatctcaccg ctaaacggca cctgacgact
    11941 ccccatttcc ggtttgttcg gatggttcgg catggtgacc atttcgcctt cgccctgata
    12001 gttttcgata accagtttca ccggatcgat aaccgccatt gcgcgcggcg cattttcgtt
    12061 gagatcttca cggatgcagg attccagcga tgccatctca atggtgttgt cctgcttggt
    12121 cacgccgatg cgtttgcaga actcacgaat agaagcagca gtgtaaccac gacgacgcag
    12181 accggaaatg gtcggcatac gcgggtcatc ccagccttca acgtgcttgt cggtcaccag
    12241 caggttcagc ttacgcttgg acatcacagt gtattccaga ttcaggcgcg agaattcgta
    12301 ctggcgcggg tgaacaggaa tggtgatgtt gtccagcacc cagtcgtaca ggcgacggtt
    12361 gtcctggaac tcaagcgtac acagagagtg cgtaatacct tccagggcat cgctgatgca
    12421 gtgggtgaag tcgtacatcg ggtagatgca ccacttgttg ccagtctggt ggtgttcagc
    12481 aaatttaata cggtacagca ccggatcgcg catcacgata aacggtgaag ccatgtcgat
    12541 tttcgcacgc aggcaggctt taccttcttc aaaaccaccg gcacgcattt tttcgaacag
    12601 cgccaggttc tcttcaacgc tgcggtcgcg gtacgggctg tttttacccg gttgcgtcag
    12661 ggtgccgcgg tattcgcgga tctgttccgg cgtcagttca tcaacgtacg ccaggccttt
    12721 attgatcagt tcgatcgcat aggcgtggag ctgatcaaaa taatcggagg agtaacggac
    12781 gttaccagac cagtgaaaac ctaaccactc tacgtcgttt ttgatcgact caacatactc
    12841 gatatcttct tttaccgggt tagtgtcgtc gaaacgcagg ttgcactggc ctttatagtc
    12901 ctgggcgatc ccgaagttca ggcagataga tttcgcatgg ccaatatgca gatagccatt
    12961 cggctccggc gggaaacggg tgtgtactgt ggtgtgctta ccactggcca gatcttcatc
    13021 gatgatctga cggataaagt tagtcgggcg ggcttctgcc tcactcatcg tggattcctc
    13081 aaagcgtaaa caacgtataa cggcgtatga tcttataagc gggacaggct gacaactgtt
    13141 agttttttaa aattgataat ctaacaagga ttatggggat gattgatgaa aaaaaaagcg
    13201 gcagagaaga tctccaccgc ttgtcattgt tggatgcgac gctcaagcgt cgcatcaggc
    13261 ataaagcaga ttactttttg atttcataca gcggtgtttg acccgccaca acatggccct
    13321 gagctttaat gatcaagcca ctgaaatcgt cgatattgct gcaaaccacc gggctaatca
    13381 tcgagcgggc gttagcgttc aggtaatcca gatccatttc cagaatcggt tgccctgcgc
    13441 ttacctgcgc accctcttcc accagacgtt taaagccttt accttccagc gctacggtgt
    13501 cgatacccat atggacgacg atctccgcgc ctttttcggt ttccaggcag aacgcgtggt
    13561 tggtgttgaa gattttcacg attgtccctg cggctggtga tacgacgatt ttatctgtcg
    13621 gtttcaccgc cacaccgtca cccaccgctt tgctggcgaa tgcttcgtca ggaacctgat
    13681 ccagtgccac gacatcaccg gtaatcggcg ataccagctc cgcgatagat accgcgtttg
    13741 gtacagcctg cggttttgct acaggcgcgg cagttgccgg agttgcttca gctgacgcag
    13801 cggctaccgg accacgggca acgactttct tcatcgcatc gccgatggat tctgctttcg
    13861 cgccaacaat cacctgaata gtctgtttgt tcagtttcac tacaccagaa gcacccagac
    13921 gtttacacat cgtatcgttg acgcgggcag agtcagccac ggtcaggcgc agacgggtga
    13981 tacaggcgtc aattgctttc aggttgtcag tgccgccaac cgcagcaata tagttggtcg
    14041 ccagttgatt gaggccttct tcagtgttgc tgttggcttc ttcagtaacg atctcgtctt
    14101 ctttatcttc acgacccggc gttttcaggt tgaacatgcg gataaccaaa ctgaacacca
    14161 cgaagtagat agcgaagaag acaacaccca taaccagcag catccagacg ttctggctgg
    14221 cggccggcag gttatacatc aacgcgtagt cgatagcccc cgctgagaaa gagaagccag
    14281 catggatacc cagcagtgtt gccacaaaca ggctgatacc ggtcagcagt gcgtgcagga
    14341 ggtacagcag cggtgcaagg aacatgaaca ggaattccag cggctcagtc acaccggtca
    14401 ggaacgcagt aacagcaaca gaaagcagca tcccgccaac catcggacga cgctctttcg
    14461 gtgctgcgaa gtacatcgcc agcgccgcac ccggcagacc gaacatcata atcgggaaga
    14521 agccggacat gaacatcccc gcggtgccgt caccggcata gaagcggttg atatcaccgt
    14581 ggaaaaccgt acccgccgcg ttggtgaatt caccaatctg gaaccaggcg atggtgttca
    14641 gcacctgatg cagaccggtt gggatcagca gacggttgat gaaaccaaag ataccggaac
    14701 ccagcgcgcc cgcagaaacg atccactcgc ctcctgcatg gatagcgtgc tgtaccggcg
    14761 gccagacgta accaaaaatg gccgccagca ccaggcagaa gaagccggtg gcaatcggca
    14821 caaagcgttt gccgccgaag aaactcagga agtccggcag tttaatatcg gaccaacggt
    14881 tataggctgc gccaccaacc agaccggtaa tgatacccgc cagtacaccc atgttaattt
    14941 ctgggttgat ggtcaccatc gctttggtta acacaaagta acctaccgca cccgccagcg
    15001 ccgccgcacc tgcgctgtct ttcgaccagc tggatgccac accgatggcg aagattaatg
    15061 cgaggttatc aaaaatcgca ccgcccgcct gggcaataaa cgcaacgtta agtaaatctg
    15121 gctgaccgaa tcgcagcaac agtgccgcca ccggcagcac cgcgataggg agctgtaacg
    15181 ccctaccgag tcgctggaaa aaacctaaaa tattcatctt attcccccta cgagaaccct
    15241 atttggctcg tttaaagccg tatttttatt ttgctgcaaa ttgtactgcc gatgttctgt
    15301 aatcagattg ttagatcatc tgctacagag tgtgtgaaaa tttaattcgt atcgcaaatt
    15361 aaacgcgtgt cttttgtgag ttttgtcacc aaatatcgtt atcatcactc ccttttactg
    15421 gctaaagcag aaaacttatt ttatcattca aaaaatcagg tcggattgac gcctgtctgc
    15481 gcaaatccag gttacgctta aagatgccta atccgccaac ggcttacatt ttacttattg
    15541 aggtgaataa tgagactgat ccccctgact accgctgaac aggtcggcaa atgggctgct
    15601 cgccatatcg tcaatcgtat caatgcgttc aaaccgactg ccgatcgtcc gtttgtactg
    15661 ggcctgccga ctggcggcac gccgatgacc acctataaag cgttagtcga aatgcataaa
    15721 gcaggccagg tcagctttaa gcacgttgtc accttcaaca tggacgaata tgtcggtctg
    15781 ccgaaagagc atccggaaag ctactacagc tttatgcacc gtaatttctt cgatcacgtt
    15841 gatattccag cagaaaacat caaccttctc aacggcaatg ccccggatat cgacgccgag
    15901 tgccgccagt atgaagaaaa aatccgttct tacggaaaaa ttcatctgtt tatgggcggt
    15961 gtaggtaacg acggtcatat tgcatttaac gaaccggcgt cttctctggc ttctcgtact
    16021 cgtatcaaaa ccctgactca tgacactcgc gtcgcgaact ctcgtttctt tgataacgat
    16081 gtcaatcagg tgccaaaata tgccctgact gtcggtgttg gtacattgct ggatgccgaa
    16141 gaagtgatga ttctggtgct gggtagccag aaagcactgg cactgcaggc tgcagttgaa
    16201 ggttgcgtga accacatgtg gaccatcagc tgtctgcaac tgcatccaaa agcgatcatg
    16261 gtgtgcgatg aaccttccac catggagctg aaagttaaga ctttaagata tttcaatgaa
    16321 ttagaagcag aaaatatcaa aggtctgtaa ttgttatccc tgccctggct ccttgctcag
    16381 ggcaatattt tttaaaatcg ggggtcagaa tgtatgcatt aacccagggc cggatcttta
    16441 ccggccacga atttcttgat gaccacgcgg ttgttatcgc tgatggcctg attaaaagcg
    16501 tctgtccggt agcggaactg ccgccagaga tcgaacaacg ttcactgaac ggggccattc
    16561 tctcccccgg ttttatcgat gtgcagttaa acggctgcgg cggcgtacag tttaacgaca
    16621 ccgctgaagc ggtcagcgtg gaaacgctgg aaatcatgca gaaagccaat gagaaatcag
    16681 gctgtactaa ctatctgccg acgcttatca ccaccagcga tgagctgatg aaacagggcg
    16741 tgcgcgttat gcgcgagtac ctggcaaaac atccgaatca ggcgttaggt ctgcatctgg
    16801 aaggtccgtg gctgaatctg gtaaaaaaag gcacccataa tccgaatttt gtgcgtaagc
    16861 ctgatgccgc gctggtcgat ttcctgtgtg aaaacgccga cgtcattacc aaagtgaccc
    16921 tggcaccgga aatggttcct gcggaagtca tcagcaaact ggcaaatgcc gggattgtgg
    16981 tttctgccgg tcactccaac gcgacgttga aagaagcaaa agccggtttc cgcgcgggga
    17041 ttacctttgc cacccatctg tacaacgcga tgccgtatat taccggtcgt gaaccgggcc
    17101 tggcgggcgc gatcctcgac gaagctgaca tttattgcgg tattattgct gatggcctgc
    17161 atgttgatta cgccaacatt cgtaacgcta aacgtctgaa aggcgacaaa ctgtgtctgg
    17221 ttaccgacgc caccgcgcca gcaggtgcca acattgaaca gttcattttt gcgggtaaaa
    17281 caatatacta ccgtaacgga ctttgtgtgg atgagaacgg tacgttaagc ggttcatcct
    17341 taaccatgat tgaaggcgtg cgtaatctgg tcgaacattg cggtatcgca ctggatgaag
    17401 tgctgcgtat ggcgacgctc tatccggcgc gtgcgattgg cgttgagaaa cgtctcggca
    17461 cactcgccgc aggtaaagta gccaacctga ccgcattcac acctgatttt aaaatcacca
    17521 agaccatcgt taacggtaac gaggtcgtaa ctcaataaga gaaagtatga caccaggcgg
    17581 acaagctcag ataggtaatg ttgatctcgt aaaacagctt aacagcgcgg cggtttatcg
    17641 cctgattgac cagtacgggc caatctcgcg gattcagatt gccgagcaaa gccagcttgc
    17701 ccccgccagc gtaaccaaaa ttacgcgtca gcttatcgaa cgcgggctga tcaaagaagt
    17761 tgatcagcag gcctccaccg ggggccgccg cgctatctcc atcgtcaccg aaacccgcaa
    17821 tttccacgca atcggcgtac ggcttggtcg tcacgacgcc accatcaccc tgtttgatct
    17881 cagcagcaaa gtgctggcag aagaacatta cccgctgccg gaacgtaccc agcaaacgct
    17941 ggaacatgcc ctgttgaatg ccattgctca gtttattgat agctaccagc gcaaactgcg
    18001 cgagctgatc gcgatttcgg tgatcctgcc agggcttgtt gacccggaca gcggcaaaat
    18061 tcattacatg ccgcatattc aggtagaaaa ctgggggctg gtagaagcac tggaagaacg
    18121 ttttaaagtg acctgtttcg ttggtcacga tatccgtagt ctggcgctgg cggagcacta
    18181 cttcggtgca agtcaggatt gcgaagactc cattctggtg cgtgtccatc gcggaaccgg
    18241 ggccgggatt atctctaacg ggcgcatttt tattggccgc aacggcaacg tcggtgaaat
    18301 tggccatatt caggtcgaac cgctgggtga acgctgccac tgcggcaact ttggctgcct
    18361 ggaaactatc gctgccaacg ccgctattga acaacgggtg ttgaatctgt taaagcaggg
    18421 ctaccagagc cgcgtgccgc tggacgactg caccatcaaa actatctgca aagccgcgaa
    18481 caaaggcgat agcctggcct cggaagtgat tgagtatgtc ggtcgtcatc tgggcaaaac
    18541 catcgccatt gctatcaact tatttaatcc gcaaaaaatt gttattgccg gtgaaatcac
    18601 cgaagccgat aaagtgctgc tccctgctat tgaaagctgc attaataccc aggcgctgaa
    18661 ggcgtttcgc actaatctgc cggtggtacg ttctgagctg gatcaccgct cggcaatcgg
    18721 cgcttttgcg ctggtaaaac gcgccatgct caacggtatt ttgctccagc atttgctgga
    18781 aaattaatgt gcttttatag tggcgcttat tgttgtcaat attctgggta gtccatgacc
    18841 attaaaaatg taatttgcga tatcgacggc gtgctgatgc acgataacgt cgccgtaccg
    18901 ggtgcagcgg aatttttgca cgggattatg gataaaggcc tgccgctggt gttgctgacc
    18961 aactatcctt cgcagaccgg gcaagatctg gcgaaccgct ttgccactgc cggtgtcgat
    19021 gtgccggata gcgtgtttta tacctctgcg atggcgactg ccgatttcct gcgtcgccag
    19081 gaaggcaaga aagcgtatgt ggtgggcgaa ggcgcactga ttcatgaact gtacaaagcc
    19141 ggtttcacta ttaccgatgt gaaccctgat tttgtgattg ttggcgaaac gcgttcctac
    19201 aactgggaca tgatgcataa agcggcctat ttcgtcgcta acggtgcacg ctttatcgcc
    19261 accaacccgg acacccacgg gcgcggtttt tatcccgctt gtggcgcgtt gtgtgcaggg
    19321 attgagaaaa tctccgggcg caaaccgttc tatgttggta agcccagccc gtggatcatc
    19381 cgcgcggcat taaacaaaat gcaggcgcat tcggaagaaa cggtgattgt cggcgataac
    19441 ttgcgcaccg acattctggc gggcttccag gcgggtctgg agacgattct ggtgctttct
    19501 ggtgtttcat cgctcgacga tatcgacagt atgccgttcc gcccaagctg gatttacccg
    19561 tcggtcgctg aaatcgacgt tatctgaata gcaaccacgt ctcagggcgt ggtttcttat
    19621 ttttacccat cattaaaatc atcccattca ataaaactcg ccaaaaatta tcgatccatc
    19681 aataaataca tcaccacacc ctcccctttt tcacaaatca gcaatcacca ttacgttttt
    19741 tatttttgca ctgccaaatc gcttgcgcac ggtgctgctt tgcggcattt ttttaaacaa
    19801 gcaaacacaa caagcaacaa ataccaggtt aacggagaag gttatgtgtt caatttttgg
    19861 cgtattcgat atcaaaacag acgcagttga gctgcgtaag aaagcactcg agctgtcacg
    19921 cctgatgcgt catcgtggcc cggactggtc cggtatttat gccagcgata acgccattct
    19981 cgcccacgaa cgtctgtcaa ttgttgacgt taacgcgggg gcgcaacctc tctacaacca
    20041 acaaaaaact catgtgctgg cggtaaacgg tgaaatctac aaccaccagg cactgcgcgc
    20101 cgaatatggc gatcgttatc agttccagac cggatctgac tgcgaagtga tcctcgcgct
    20161 gtatcaggaa aaagggccgg aatttctcga cgacttgcag ggcatgtttg cctttgccct
    20221 gtacgacagc gaaaaagatg catatctgat tggtcgcgac catctgggga tcatcccact
    20281 gtatatgggc tatgacgagc acggtcagct gtatgtggcc tcagaaatga aagccctggt
    20341 gccagtttgc cgcacgatta aagagttccc ggcggggagc tatttgtgga gtcaggacgg
    20401 cgaaatccgt tcttattatc atcgcgactg gttcgacttt gatgcagtga aagataacgt
    20461 gaccgacaaa aacgagctgc gtcaggcact ggaagattcc gttaaaagcc atctgatgtc
    20521 tgacgttcct tacggtgtgc tgctctcggg tggtctggat tcctcaatta tttccgcgat
    20581 caccaagaaa tacgccgccc gtcgcgtgga agatcaggaa cgctctgaag cctggtggcc
    20641 gcaattacac tcctttgctg taggtctgcc gggttcaccg gatctgaaag cagcccagga
    20701 agtggcaaac catctgggca cggtgcatca cgaaattcac ttcactgtac aggaaggtct
    20761 ggatgccatc cgcgacgtga tttaccacat cgaaacctat gatgtgacaa ctattcgcgc
    20821 ttcaacaccg atgtatttaa tgtcgcgtaa gatcaaggca atgggcatta aaatggtgct
    20881 gtccggtgaa ggttctgatg aagtgtttgg cggttatctt tacttccaca aagcaccgaa
    20941 tgccaaagaa ctgcatgaag agacggtgcg taaactgctg gccctgcata tgtatgactg
    21001 cgcgcgcgcc aacaaagcga tgtcagcctg gggcgtggaa gcacgcgttc cgttcctcga
    21061 caaaaaattc cttgatgtgg cgatgcgcat taacccgcag gataaaatgt gcggtaacgg
    21121 caaaatggaa aaacacatcc tgcgtgaatg ttttgagtca tacctgcccg caagcgtggc
    21181 ctggcggcag aaagagcagt tctccgatgg cgtcggttac agttggatcg acaccctgaa
    21241 agaagtggcg gcgcagcagg tttctgatca gcaactggaa actgcccgct tccgcttccc
    21301 gtacaacacg ccaacctcaa aagaagcgta tctgtaccgg gagatctttg aagaactgtt
    21361 cccgcttccg agcgccgctg agtgcgtgcc tggcggtcct tccgtcgcgt gttcttccgc
    21421 taaagcgatt gagtgggatg aggcgttcaa gaaaatggac gatccgtctg gtcgtgcggt
    21481 tggcgttcac cagtcggcgt ataagtaaga aatgcgaaac agccccggtg aaatccgggg
    21541 ctgtttcagt tattgaggtg cttgttaatt ctgatatgca gtaaggaaat gttccgggtt
    21601 tgccacatca ctgtttgaca actcatcact gtaggccagt atttcataga ttaatttccc
    21661 accactattg ttgaattccg aatttcatcc tgtacgacaa aagaatggct gtagggtggg
    21721 gaaaaaacac aaagtaaaca ataattgacg aatatagcgc cacgctgttc gcaacctaac
    21781 caaacagtca ctttcgagca attttccttg aaaaagaggt tgacgctgca aggctctata
    21841 cgcataatgc gccccgcaac gccgataagg tatcgcgaaa aaaaagatgg ctacgtagct
    21901 cagttggtta gagcacatca ctcataatga tggggtcaca ggttcgaatc ccgtcgtagc
    21961 caccatcttt tttgcgggag tggcgaaatt ggtagacgca ccagatttag gttctggcgc
    22021 cgcaaggtgt gcgagttcaa gtctcgcctc ccgcaccatt caccagaaag cgttgtacgg
    22081 atggggtatc gccaagcggt aaggcaccgg tttttgatac cggcattccc tggttcgaat
    22141 ccaggtaccc cagccatctt cttcgagtaa gcggttcacc gcccggttat tggggtatcg
    22201 ccaagcggta aggcaccggt ttttgatacc ggcattccct ggttcgaatc caggtacccc
    22261 agccatcgaa gaaacaatct ggctacgtag ctcagttggt tagagcacat cactcataat
    22321 gatggggtca caggttcgaa tcccgtcgta gccaccaaat tctgaatgtg tcgaatacgt
    22381 tcggcaaatt caaaaaccaa tttgttgggg tatcgccaag cggtaaggca ccggattctg
    22441 attccggcat tccgaggttc gaatcctcgt accccagcca atttattcaa gacgcttaca
    22501 tcgtaagtgc gcccagttgg ggtatcgcca agcggtaagg caccggattc tgattccggc
    22561 attccgaggt tcgaatcctc gtaccccagc cacattaaaa aagctcgctt cggcgagctt
    22621 tttgcttttc tgcgttcatt caatgtcgaa tgcgatgttg acacgtctta tccttcaatg
    22681 tcggatgcga cgctgccgcg tcttatccga cctacggttg gcacgcatcc ggcaatgttg
    22741 taaggctaca accctaacgc atatttcagc gcctgacgtt tcaacacgcc agcacgctct
    22801 gccgccatta acccgagatt acgcacaaaa cgcagcggcg gcagattatt actgaatccg
    22861 gcataaaaca gatccatacc actttgcata atgaagttat ccgccatgcg ccgcatctgg
    22921 taacgcttga ggacaggata actggcccac gcttcaccgt agctgcgggc attcaccaga
    22981 acatcaatca gggcatcgac atcacgataa ccaagattcc ccccctgccc cgccagcgga
    23041 tggatggtat gcgcggcatc gcccaccagc gcaagccccg gctgcacata ctgcaacgca
    23101 tgtcggcgcg tcagcggaaa cgcaccagcg gcaagcggtg taacgtaacc cagacgcgac
    23161 gggaaatgct tcgcgatttc cgcctggagt tgcgccatat tcatattctg caactggcga
    23221 atacgtaccg gagagtcata ccacaccagc gatgcccagt tatcaaacaa cggcagaaac
    23281 gcacgcggtc cgtccggggt aaattgctgc caggtgctgt cgccgggatc gttctcacac
    23341 tgtacgctaa tcaacataca cgactgcgca tactgccagg catgaacgcc aattcccgcc
    23401 atctgccgca cctgcgaatt tgcgccgtca gcaccaatca ccagcttcgc gcgaatcact
    23461 tcaccgccct tcagttccag ctcctgaaga tcattatctc gatgtaatgc aatcagcgag
    23521 cctggcacac gtaacgttac tttcggatgc gcttccagcg cctgccacaa tgcctgttgc
    23581 aggacggtgt tttccaccat atagccaagc agcggcagct taagttcagc ggcgtcaaac
    23641 atcacatgcg ccgtttccca ctcccacgtt tccagtctgc ggtaaggatg gcaacgcata
    23701 gcctgtactg catcccagac ccctaaccct ttaagcaatg ataccgatgc ggcgctgatc
    23761 gccgagatcc gcacgtccgg ttggctatca gcgacaaacg gtgcaggttc tgcgtgctcg
    23821 atcaccgtta ccgaaaatcc gtgctgtgcc agccccagcg ccagtgcgcc gccgaccatt
    23881 cctccgccga caatggcaat ttccgttggt tgatttgtca tggtcaatca tcctgttagc
    23941 aatatgcttt aagtttacag gatttttacc cccaagggct gataacgctc atactggtca
    24001 caacggcagc aaagcattac actatgcgcc ctgcattcct ggctactatt tcgcaagagc
    24061 aagtcgatga ccaaaaaact ccatattaaa acctggggct gtcagatgaa cgagtacgat
    24121 tcatcgaaga tggccgatct gctggatgcc acccacggct atcaactgac cgacgtggcg
    24181 gaagaagcgg atgtgctgct gctgaacacc tgctcaatcc gcgagaaggc tcaggaaaaa
    24241 gtcttccatc agttgggtcg ctggaaactg ttaaaagaga agaatccaga cctgattatc
    24301 ggcgtcggtg gctgcgtggc atcgcaagaa ggcgagcaca ttcgccagcg cgcccactat
    24361 gtcgatatta tttttgggcc gcaaacgctg caccgtctgc cggagatgat caactccgtg
    24421 cgcggcgacc gcagcccggt tgtagatatc agcttcccgg aaatcgagaa gtttgaccgt
    24481 ctgccggaac cgcgcgccga agggccgacc gcgtttgtct ccatcatgga aggctgcaat
    24541 aaatattgca cctactgcgt ggtgccttac acccgtggtg aagaggtaag ccgtccgtcc
    24601 gacgatattc tgtttgagat cgcccagctt gcggctcagg gcgtgcgtga agtcaacctg
    24661 ctcggtcaga atgtgaatgc ctggcgtggt gagaactacg acggcaccac cggatcgttt
    24721 gccgatctgt tgcgtctggt tgctgcgatc gacgggatcg atcgtattcg ctttaccacc
    24781 agccatccga tcgaattcac cgacgatatc atcgaagtgt atcgcgacac gccggagctg
    24841 gtgagcttcc tgcatctgcc ggtacagagc ggttccgatc gcattctgaa cctgatgggc
    24901 cgtactcata cggcactgga gtacaaagcg atcatccgta aactgcgtgc ggcgcgtccg
    24961 gatattcaga tcagttctga cttcattgtt ggcttccctg gcgaaaccac cgaagacttc
    25021 gagaaaacga tgaagctgat tgccgacgtc aatttcgaca tgagctacag ctttatcttc
    25081 tctgcacgtc cgggtacacc agccgccgat atggttgatg atgttccgga agaagagaag
    25141 aagcaacgtc tgtatattct gcaagaacgt atcaatcagc aagcgatggc atggagccgc
    25201 cggatgctcg gcaccaccca gcgtattctg gtagaaggga cttcgcgtaa gagcatcatg
    25261 gagctttccg ggcgtaccga aaataaccgc gtggtcaact tcgagggcac accggatatg
    25321 atcggtaaat tcgtcgatgt agaaattacc gacgtctacc cgaactctct gcgcggtaaa
    25381 gtggtgcgta ctgaagatga aatgggtctg cgcgtggcag aaacaccgga atcggtgatt
    25441 gcccgtaccc gcaaagaaaa cgaccttggc gtgggttatt atcagccgta attctcaggc
    25501 ccgccgttcc ggtgggcctt gttttttctc tctttatccc catctttatc gcaatgcttg
    25561 cgcctttttc ctgtggcgtg aataatttcc ttataggccg gtgaatgttc agcttccggc
    25621 gacatacaga taagaggaac ggtttgaaca tagacactcg cgaaatcacc ctggagccag
    25681 cagacaacgc gcgtctgttg agcctgtgcg gcccgtttga tgacaacatc aagcagctcg
    25741 aacgccgtct cggcatcgag atcaatcgcc gcgacaacca ctttaaactg accggccgtc
    25801 cgatttgcgt caccgctgcg gcagacattc tgcgtagcct gtatgtcgat acagccccga
    25861 tgcgcggtca gattcaggat atcgaaccgg aacagatcca ccttgcgatt aaagaagcgc
    25921 gagtactgga gcaaagtgcg gagagcgtgc cggagtacgg caaagcggtc aatatcaaaa
    25981 ccaaacgcgg cgtaattaag ccgcgcacgc caaaccaggc gcagtacatc gccaatattc
    26041 tcgaccatga cattaccttc ggcgttggcc cggcgggtac gggtaaaacc tacctggcag
    26101 tggctgcggc agttgatgcc ctggagcgtc aggagattcg ccgtattctg ctgactcgtc
    26161 cggcggtcga agccggtgag aaactgggct tcctgcctgg cgatttaagc cagaaagtag
    26221 acccgtatct gcgcccgctg tacgacgcgc tgtttgaaat gctgggcttt gagaaagttg
    26281 agaaactgat tgagcgcaac gttattgaag tcgcgccgct ggcctatatg cgtggtcgta
    26341 cgctgaacga cgcgtttatc attctcgatg agagccagaa caccaccatc gaacagatga
    26401 agatgttcct gacccgtatc ggttttaact caaaagcggt tatcaccggc gacgtcacac
    26461 agatcgacct gccgcgtaat actaaatcag gcttacgtca cgccatcgaa gtgctggccg
    26521 atgtcgaaga gatcagcttt aacttcttcc acagcgaaga cgtggttcgt cacccggtgg
    26581 tggcgcgtat cgttaacgcc tatgaagcct gggaagaagc cgaacaaaaa cgaaaagcgg
    26641 cgctggcggc agaacgcaag cgcgaagaac aggaacaaaa atgagtcagg tgatcctcga
    26701 tttacaactg gcatgtgaag ataattccgg gttaccggaa gagagccagt ttcaaacatg
    26761 gctgaatgcg gtgatcccgc agtttcagga agaatcggaa gtgactattc gcgtggtcga
    26821 taccgccgaa agccacagtc tgaatctgac ctatcgcggt aaggataagc cgaccaacgt
    26881 gctctccttc ccgtttgaag tgccgcctgg catggaaatg tcgctactgg gcgatctggt
    26941 tatctgccgt caggtggttg agaaggaagc tcaggagcaa ggcaaaccac tggaggcgca
    27001 ctgggcgcat atggtggtgc acggcagtct gcatttgtta ggttacgatc acatcgaaga
    27061 tgacgaagca gaagaaatgg aagccctcga aacagagatt atgcttgctc tgggctatga
    27121 ggatccgtac attgccgaga aagaataatt tgccaggcat ttgactggca gacgtccccg
    27181 ccgttggtga ttaacgacgg cgttttgatt aactaacatg agaacccata cgacgccatg
    27241 agcgacgaca attcacacag tagtgacacg ataagcaaca agaagggatt tttctccctg
    27301 ttactcagcc aacttttcca cggtgaaccg aaaaaccgtg acgaactgct ggcgctgatc
    27361 cgtgattccg ggcagaacga ccttatcgac gaagatacgc gcgatatgct cgaaggggtg
    27421 atggacatcg cagaccaacg cgttcgcgac atcatgatcc cccgctccca gatgattacc
    27481 ctgaaacgca accagacgct ggacgaatgc cttgatgtca tcatcgagtc cgcccactca
    27541 cgtttcccgg tgattagcga agacaaagat cacattgaag ggattctgat ggcgaaagac
    27601 ttgctaccgt ttatgcgcag cgatgctgaa gccttcagca tggacaaagt gttacgtcag
    27661 gcggttgtcg ttcctgaaag taagcgcgta gaccggatgc tgaaagagtt tcgctctcag
    27721 cgttaccaca tggcgatcgt tattgacgaa ttcggtgggg tttccggtct ggtgaccatt
    27781 gaagacatcc tggaactgat tgttggtgag attgaagacg agtatgacga agaagatgat
    27841 atcgacttcc gtcagctgag tcgtcatacc tggaccgtgc gcgcactggc ttccattgaa
    27901 gacttcaacg aagcgttcgg cacccacttt agcgatgaag aagtcgacac tatcggtggt
    27961 ctggtgatgc aggcgtttgg gcatcttccg gcgcgtggcg aaactatcga catcgacggt
    28021 taccagttca aagtggcgat ggccgatagt cggcgtatta ttcaggttca tgtcaaaatc
    28081 ccggatgact caccccagcc aaagctggat gaataaaacc gaaactggat agataactac
    28141 atggcttttg cctcattaat tgaacgccag cgcattcgcc tgctgctggc gttattattc
    28201 ggtgcctgcg gaacgctggc cttctctcct tacgatgtct ggcctgcggc gattgtttcg
    28261 ctgatggggc ttcaggcgct gacctttaac cgccgcccgc tccagtctgc cgctattggc
    28321 ttctgctggg gatttggcct ctttggcacc ggtattaact gggtctatgt cagcatcgcg
    28381 acctttggcg ggatgcctgg cccggttaac atcttcctgg tggtgctgct ggcggcgtat
    28441 ttgtcgctgt ataccggact gtttgctggc gtgctgtcgc gcctgtggcc gaaaaccacc
    28501 tggctgcgcg tggcgattgc cgcccctgct ctctggcaag tgaccgagtt tctgcgcggt
    28561 tgggtgctga ctggcttccc gtggttacag ttcggctata gccagattga tggcccgtta
    28621 aaagggctgg caccaataat gggcgtggaa gccattaact tcctgctgat gatggttagc
    28681 ggcctgctgg cactggcgtt agtcaaacgc aactggcgtc cgctggtggt ggccgtcgtg
    28741 ctgtttgccc ttcccttccc gctgcgttac atccaatggt ttaccccgca accggagaaa
    28801 accattcagg tttcgatggt tcagggcgat attccgcaat cactgaaatg ggacggagac
    28861 cagcttctta atacgctgaa gatttactac aacgcaacgg caccgctgct gggcaaatca
    28921 tcgttgatta tctggccgga gtcggcgata accgatctgg aaattaatca gcaaccgttc
    28981 ctcaaagcac tggacggtga gttgcgtgat aaaggtagct cgctggtaac cgggattgtc
    29041 gacgcgcgtc tcaataagca gaaccgctac gatacctaca acaccatcat cacgctgggt
    29101 aaaggtgcac cgtacagcta cgaatcagcc gatcgctata acaaaaacca tctggtgccg
    29161 tttggcgagt ttgtcccgct ggagtcgatt ctgcgtccgt tagcaccgtt ctttgatctg
    29221 ccgatgtcgt cgttcagccg tgggccatat atccagccgc cgctgtcggt aaatggtatt
    29281 gagcttactg cggccatttg ctacgagatc attcttggcg agcaagtgcg cgataacttc
    29341 cgcccggata ccgactatct gctgactatc tccaacgatg cgtggtttgg taagtctatt
    29401 ggtccatggc agcacttcca gatggcgcga atgcgtgcgc tggagctggc gcgcccactg
    29461 ttgcgcagca ccaacaacgg cattacggcg gtgattggcc cgcagggtga gattcaggcg
    29521 atgatcccgc agttcacccg cgaggtgttg accactaatg tgacaccgac caccggactc
    29581 acaccgtatg cacgtaccgg caactggccg ctgtgggtgc tgacggcgct gtttggtttt
    29641 gccgctgtgc tgatgagtct gcgagcacgt aaacgttgat gtgcctgatg tatcctgcat
    29701 gagcaaaacg tactttctca ttgagctttg gggcatggtt atcatgcccc tttttcttac
    29761 ggttcaggga tacaagccac attgccatat tggggatgac gaataattgc ctgcggatca
    29821 agcgagcgcg tcctgtccat ttggatcaac ccgttcttcg ccatgacaaa tatttttcta
    29881 ccatccgctg aaaaacgaag gtgattaacg tagctggcag gataaacgcc aataatctgc
    29941 ccggcattaa tattgagcag gatgacataa ccttgcaggc catatgacac caacgctaaa
    30001 tattgaccgt ccgggctaat accctcaacg gaataaacgc tcctgctaca ccattgcgtc
    30061 ataatgctgg cttgttgatc ccctacgctt atacgtgaaa tcccctgact atcggcaaaa
    30121 ataacctcgc cgttttcagg tcgaaaacgc atcgccgata tcgttccacg cgcatcacct
    30181 gcgggaaagt cttctttctg atgcgtgatg gtattgatcc gcacgatgtt acctctgcct
    30241 gctcccggaa ccagtaatgt tctttcatcc ggggaaagtg ccataaccca ggcgcctggt
    30301 ttaccgctcg tccccgtccc gtcgtatttc tccagcggga tatccgccac atgctgataa
    30361 gtcaccagat caatcgctga cacactatta ctggtgcgat tcatcaccca ggcttttgtg
    30421 ccatcacgcg agaggataaa actgtcagga tgtgcaccgg tggtaattga ctgaataatc
    30481 tcccctgtcg ccacgttaat gaccgataac gcattcgctt caaatgacgt cgcataaatc
    30541 aacttttcat ccgggcttaa tgccagcgat ctgacgccct gatctttaca gccaccacaa
    30601 tgtttgactg acttttcttt cgctatgggc gctgtaaagg tatgcaggat tttcttttgt
    30661 ccgaggtcga aaacaacaat gctgttggtg ttttcaattg cggcatacaa cgtattacca
    30721 tcccgactga tcgccatatc atggatatcg gcaatctcag cgagtaaatc accactggcc
    30781 tgttcacgac ttaccggagc ggataatgtt tccctgaggg cgttgcgttc ctccgctaac
    30841 tgttgttttt tttgtcgtcg ctcttgttca atctcctgcg gataaaacgc ttcacgcagg
    30901 ctttgcctga cctgtaaaac tgactcatcc atctgctgct gcgcaagcca gatagccccg
    30961 gtcagaagaa gactgcccgc aacaataatc ccgccttccg taacgagtct gttttgccca
    31021 gataaacgcg cgctcagcca acccaacgcg ccacccgcaa tcaggccgcc aatgtggcaa
    31081 gcgttatcta ttcctgtttg ccgggcaccg tacaacagtg tcagcgccac catacccaac
    31141 agaggaaata ctcgccgctg gtttttatgg tatgtgcccg tacctgaagc aaactgtgtt
    31201 gctatcgctg caccagctat tcccagaatc gccccggatg ccccaacgct gatgactatc
    31261 tgctcacttt cgcgtaacgc ataacaggcg ctgatgagcc caccaaagac gccggagaat
    31321 aaccaggtaa tcaggagtcg ccatttcccc agaatctgtt ccgccacgcc tcccacgacc
    31381 aggaatgcaa acatattcat cagcagatga gaaacactac tgtgcagaaa gatactgctc
    31441 aataagcgcc agggttcacc cgacaaagta agaggcgcaa cgtttgcacc gagatggata
    31501 agtgcggatt gatcgacctc ttcataactc atccattgtg cgagataaca aaatatccca
    31561 atacagagtg caaaaaggat aagggttatt gcgggtaatt gaacatttaa cggcttaacc
    31621 gaagatgcag acataaggtg tttcttcctt gaaaagacag gtaaaaagca tttttcccgt
    31681 gtgcagttat catcccggca aacgtcccat gccgaaaaga taattctgtc cgaaatatga
    31741 ctttttcagc ctaaaattac aatcgtaatt aattcgcccc taagtatttt ttggcatgta
    31801 agttgcttta tttactgtgt aagcgatgaa atcagccgcg acgcaaccag gttgcaccac
    31861 cacaaatcag cggtagcacc aaaaaggtgc ggtttaagaa ttttgcttcg gaatggtgcg
    31921 gcgcatctcg caaaaacaaa caataaaaca gcaatatctt tacatttagc caaactaaat
    31981 gttacctaac aggcacaaca ctgcacaata aagttgcaga cgataacaac acaaacactc
    32041 acaacgggta tccatgcgtt cttaacgcag aagataaagg agttggatat gcaattacgt
    32101 aaacctgcca cagcaatcct cgccctggcg ctttccgcag gactggcaca ggcagatgac
    32161 gccgccccgg tagcgggcag tacgctggac aaaatcgcca aaaacggcgt gattgtcgtc
    32221 ggtcaccgtg aatcttcagt acctttctct tattacgaca atcagcaaaa agtggtgggt
    32281 tactcgcagg attactccaa cgccattgtt gaagcagtga aaaagaaact caacaaaccg
    32341 gacttgcagg taaaactgat tccgattacc tcacaaaacc gtattccact gctgcaaaac
    32401 ggcactttcg attttgaatg tggttctacc accaacaacg tcgaacgcca aaaacaggcg
    32461 gctttctctg atactatttt cgtggtcggt acgcgcctgc tgaccaaaaa gggcggcgat
    32521 atcaaagatt ttgccgacct gaaaggcaaa gccgtagtcg tcacttccgg cactacttct
    32581 gaagttttgc tcaacaaact gaatgaagag caaaaaatga atatgcgcat cattagcgcc
    32641 aaagatcacg gtgactcttt ccgtaccctg gaaagcggtc gtgccgttgc ctttatgatg
    32701 gatgatgccc tgctggccgg tgaacgtgcg aaagcgaaga aaccagacaa ctgggatatc
    32761 gtcggcaagc cgcagtctca ggaagcctac ggttgcatgt tgcgtaaaga tgatccgcag
    32821 ttcaaaaagc tgatggatga cactatcgct caggtgcaga cttccggtga agcggaaaaa
    32881 tggtttgata aatggttcaa aaatccaatt ccgccgaaaa acctgaacat gaatttcgaa
    32941 ctgtcagacg aaatgaaagc actgttcaaa gaaccgaatg acaaagcact gaactaatta
    33001 caagaaccag gggcggaaat tccagccctc tcgattgtta cgtagcacgg acagactata
    33061 cgcctgatgg tcgttcccca tcgggcctga aaaccgcaat acgctgggta acaatcttcg
    33121 agggtagcag ttaacgctgc taccctcttt tttctggagt agatttatgt ctatagactg
    33181 gaactggggt atttttttac aacaagcccc gttcggcaac accacctatc ttggttggat
    33241 ctggagtggt tttcaggtga cgatcgcttt gtcgatctgc gcctggatta tcgctttcct
    33301 cgtcggttca tttttcggca ttttacgtac cgttccaaac cgttttctct ccggtctggg
    33361 tacgttgtat gtcgaactgt tccgcaacgt gccgttgatt gtgcaattct ttacctggta
    33421 tctggtgatc ccggagttgc tgccagagaa aatcggtatg tggtttaagg ccgagctgga
    33481 tcccaatatt cagtttttcc tttcatccat gctctgcctg gggctgttta ccgccgcccg
    33541 tgtttgcgaa caggtacgcg ccgccattca gtcgttgccg cgtgggcaaa aaaatgccgc
    33601 gctggcgatg ggcctgacgc taccgcaggc ttatcgatac gtactgctgc ctaatgctta
    33661 tcgcgttatc gtcccgccga tgacttccga gatgatgaac ctggtgaaaa actccgccat
    33721 cgcctcgact attggtctgg tggatatggc ggcgcaggcg ggtaaattgc ttgattactc
    33781 agcccacgcc tgggagtcgt ttaccgctat tacgctggcg tatgtgttga ttaacgcggt
    33841 tatcatgctg gtaatgacgc tggttgaacg taaagttcgc ctgcctggca acatgggggg
    33901 caaataatgt acgagtttga ctggagttcc attgtccctt ccctgccata tctgctcgac
    33961 gggctggtga tcacgctgaa aatcaccgtc acggcagtcg tgattggtat tttgtggggc
    34021 accatgctgg cggtgatgcg cttgtccagc tttgcgcccg tagcgtggtt tgccaaagcc
    34081 tacgttaacg tattccgctc gattccttta gttatggttt tgctgtggtt ttacctgatc
    34141 gtgccaggtt ttctgcaaaa cgtgctggga ttatcaccaa aaaatgatat ccgtttaatt
    34201 tcggcgatgg tggcgttttc aatgtttgaa gcggcctact attcagagat tatccgtgct
    34261 ggtattcaaa gtatttctcg tggtcagtcg agcgccgcgc tggcgttggg aatgactcac
    34321 tggcagtcga tgaaactgat tattctgccg caggcgttcc gcgcgatggt gccgcttctg
    34381 ctcactcagg gcatcgtact gttccaggat acctcactgg tgtatgtgtt atctctggcc
    34441 gatttcttcc gtaccgcctc aaccattggt gagcgtgatg gtacgcaggt tgagatgatc
    34501 ctatttgccg gatttgttta tttcgttatt agtcttagcg cgtcgttgtt ggtcagctac
    34561 ttgaaaagaa ggacagcata atgattaccc tgaaaaatgt ttcaaaatgg tatggtcact
    34621 ttcaggtgct gaccgactgc tcaaccgaag tgaaaaaagg cgaagtggtg gtggtttgcg
    34681 gcccgtctgg ttccggcaaa tcaacgctga ttaaaaccgt caacggcctc gaaccagtgc
    34741 agcaaggtga aatcaccgtc gatggtatcg tggttaatga caagaaaacc gatctggcaa
    34801 agctgcgttc ccgcgtcggg atggtgttcc agcattttga gttgttcccc catttgtcga
    34861 ttattgaaaa cctgactctg gcgcaggtga aagtgcttaa acgcgataaa gcgcccgctc
    34921 gtgaaaaagc attgaaattg ctggagcgcg tgggactttc cgcacatgcc aataaatttc
    34981 cggcgcagct ttccggcggt cagcagcagc gtgtggcaat cgctcgcgcg ttgtgtatgg
    35041 atcctattgc gatgctgttc gacgaaccga catcggcgct ggatccggag atgatcaacg
    35101 aagtgctgga cgtgatggtg gaactggcga acgaaggaat gaccatgatg gtggtgaccc
    35161 acgaaatggg ctttgcccgc aaagtggcga atcgggtgat ctttatggac gagggtaaaa
    35221 ttgtcgaaga ctcgccgaaa gacgctttct tcgatgatcc gaaatcggac cgcgcgaaag
    35281 acttcctcgc gaaaatcctg cattaatcac tctggcgcgc atacccaagt gcgcgccatc
    35341 tcacgcttta tcgtttcgcc cctcgtatct gttacatcct ggcgttaccc ttgtcgcaaa
    35401 gaagcacaca acaaggagca acaatggcac tgccaattct gttagattgc gacccaggtc
    35461 atgacgacgc tatcgcaata gttctcgccc tcgcctcacc agagcttgat gtcaaagcaa
    35521 ttacgtcttc cgccggaaac cagacaccag aaaaaacctt acgcaatgtt ctgcgtatgc
    35581 tgaccttgct taatcgcacc gatattccgg tagcaggcgg cgcggtaaaa ccgttaatgc
    35641 gtgagttgat tatcgcggac aatgtgcacg gcgaaagcgg tctggatggc ccggcattac
    35701 cggaaccgac gttcgcaccg caaaactgta cggcggtaga gctgatggcg aaaacgctgc
    35761 gcgaaagtgc ggaacctgtc acgattgtgt ctaccggacc gcaaactaac gttgccttgc
    35821 tgctcaatag ccatccggaa ctgcatagca aaattgcccg tatcgtgatt atgggcggcg
    35881 caatggggct tggtaactgg acgcctgcgg ctgaatttaa tatttacgtt gacccggaag
    35941 cggcagaaat tgtcttccag tcagggatcc cagtggtgat ggccggtctg gatgttactc
    36001 acaaagcaca aatccacgtt gaagacaccg agcgtttccg cgcgattggc aaccctgttt
    36061 caaccattgt tgccgaactg ctggatttct tcctcgaata tcataaagac gaaaaatggg
    36121 gctttgtcgg tgcaccgctg catgacccat gcaccatcgc ctggctgttg aaacccgagt
    36181 tgtttaccac tgttgagcgc tgggttggcg tggaaacaca ggggaaatat agccagggta
    36241 tgacggttgt tgattattat tatctgactg gcaataaacc gaatgccact gtaatggtcg
    36301 atgttgatcg tcagggcttt gttgatttac tggctgatcg tctgaaattt tacgcttaac
    36361 acacgtcatt gattaagatc accacgcgtt acctgggtgg tcttaatctg tttatctctt
    36421 tttctttcaa gatataaatg caatggataa tgcagaactc gccattggta tcgatctcgg
    36481 cactaccaat agtttaattg ccgtctggaa agacggtgcc gcgcaattaa ttccaaataa
    36541 gttcggtgaa tatttaacac catccataat tagcatggat gaaaataatc atattttagt
    36601 cggaaaaccg gctgtatcac ggcgtacttc gcatccggat aaaacggcag cgttatttaa
    36661 acgtgcaatg ggcagtaata ccaactggcg gttaggcagc gacacattta acgcgccaga
    36721 actgtcctct ttggtattac gctcattaaa agaagatgcc gaagaatttc tgcaacgtcc
    36781 gattaaagat gtggtgatct ccgttccggc ttatttcagc gatgaacaac gcaagcatac
    36841 ccgtttagca gcggagttag ccgggttaaa tgcggtacgc ttaattaatg aacccacagc
    36901 agctgcgatg gcgtatggcc tgcataccca acaaaatacc cgttcgctgg tgtttgatct
    36961 cggtggcggc acgtttgacg ttacggtgct tgagtacgcc acgccggtga ttgaagttca
    37021 cgcctccgct ggcgacaact ttcttggtgg cgaagatttt acccatatgc tggtcgatga
    37081 ggttttaaaa cgcgcggatg tcgccaggac cacgcttaac gagagtgaac tgggagcctt
    37141 gtacgcctgt gtggaagcgg caaaatgtag caatcaatcg ccattgcaca ttcgctggca
    37201 gtaccaggat gaaacgcggg agtgcgaatt ttacgagaac gaactggaag atttgtggtt
    37261 gccgctgctc aatcgcttgc gagtgccgat tgaacaggcg ttgcgcgatg cacgtctgaa
    37321 gccgagtcaa atcgacagtc tggtgctggt tggcggcgcg tcacaaatgc cgctggtgca
    37381 gcgaatcgcc gtgcgtctgt ttggcaaatt accgtatcaa agttacgacc cgagcaccat
    37441 tgtcgcgctg ggcgcagcaa tccaggccgc ctgccgctta cgcagtgaag atattgaaga
    37501 ggtgatcctc actgatattt gcccttactc gttgggcgtt gaagttaacc gccagggcgt
    37561 ttccggcatt ttctcgccaa ttattgaacg aaacaccaca gtacccgtgt cgcgtgtaga
    37621 aacttactcg accatgcacc cggaacagaa ttcaattacg gttaacgtct atcagggaga
    37681 aaaccacaaa gttaaaaata acattctggt tgaatccttc gatgtgccgt tgaagaaaac
    37741 cggggcttat cagtcgattg atattcgctt tagttatgat atcaacggct tgcttgaagt
    37801 tgacgtgctt ctggaagatg gcagcgttaa gtccagagtc attaatcata gcccggtaac
    37861 attgagcgcg cagcagattg aagagagtcg gacgcggtta tccgcattga aaatttatcc
    37921 gcgcgatatg ctcatcaacc gcacctttaa ggccaaactg gaagagttgt gggcgcgggc
    37981 gctgggtgac gagcgagaag agattggccg ggtgatcacc gattttgatg cggcgttgca
    38041 gtcaaacgat atggcccgcg tcgatgaggt caggcggcgg gcgagcgttt atttagccat
    38101 tgagacctca taatcacggc tgccggatgc ggcgtaaatg ctgcatccgg catcggcgca
    38161 aaaaacacat cgtcaactaa acaacatcgc gattttgccg agagcataac ctataaccag
    38221 caccgttaaa ctgattttga taatgctggt aatgctctgt ggtttttcac cctcatcatt
    38281 tttttcttca tggttttcag gtgttggctc atcgacatct tcatcctccg gctcgagttt
    38341 tactttcgtc aacagcatcc gggtgataat ggcctctaat ggatgagcat ctccgccttc
    38401 cagccttgta ttaagacggg ccagcatctc ttgtttcgga aacgcttcaa gtactacctg
    38461 ttccgctacc cgatacaaat ccagtaacgg tgaatcttct tctgtctcgc taccgactct
    38521 cgatttccac cacgcccatg ctttgcgttt gaagaataac gactcaataa acgattgacc
    38581 taacggcgat aattttccgc tatgtaaaat ttccgcccat aacagccgcg tccgggcgct
    38641 ctcaccagga acatatttca gcggttgacc ttgcgcatct accatgctcc gcgcttcgat
    38701 aaccatcact aataacgcga aataatccgg gcaatgacgc ttgctccact gcaacaacaa
    38761 attatcagct tgtgtagatg ggtaactttc ccggtacgcg cagagatcgg caagcaggct
    38821 ttccccctcg ccgcagtaca acctttgagc acacaagtaa tatcgggcat cttcgctttc
    38881 tggttcttcc atttgccact gtttggctac aggaatgagt tccgccatac ctgtttgcac
    38941 cgagctgtac cagcgcaaca gtttgcgatg taactttgca tcatcaggaa tgaaccacgg
    39001 accttccatc gccaaaaacg cagtgacgta gtcaggatgg taacggcagc aacgttccag
    39061 cgcgaagtaa tactcaacag tctggttctg caacgcaacg ggatattcca gaaggctaag
    39121 gaaatcgaaa caatcaccag cctgaatggc ctcaagaaaa gcctcccttt cttcctcatc
    39181 cattccttcg ctgtcattaa acgaatgcca gttcaaacgc tctgccagca aagaagcaca
    39241 atttaatgaa agatatcgcg cttctatagc gattgcacac agcggccagc gtaattgatc
    39301 gacatcctcc atgttccagg tatttaattg ctgaataaat ttctgccagg cggagggctg
    39361 aaaacgatca ctttctgaat ccagtaatgt cctgaatgca cgcagtattt catgttcagc
    39421 ggcggcatct ttttcttcat catctgcttc ctcgacagga tttaccgcca gtcgcagcgc
    39481 ctcttcatag gcctggcgta atgctttaaa cccctgcgga tcggtttcgg gatgacataa
    39541 cggtaagcga gcaagataag cctggcggat aatgtctatt tgcgtcgtgc tttcaatttc
    39601 gaggatttgc caacatgttt tcataggtcg ctcacatcct taagcgttac cgggcattca
    39661 ggctggacag ttaatggctg ccagggaagc gtggcataga aacgatcgct tcccgctgcc
    39721 agctcttcga acatccgact cccggcgcta tcgtatttgt acagggttag cgcctcgcgt
    39781 agcgaagtgt tgtcttcgca ctgagaagat tgccagtaga gacgtcctaa cgaataggcg
    39841 gaaaaatagt gcatccagct gtgatagtaa tggtgagctc gtacatagac gcacgattgc
    39901 agccataaac tctcttcttc cgtcagccag ttgttgagca caccgacccg tgacagaaac
    39961 cccattcgaa cataatccca ggctttgatt cccccttctc cacagcacat agcggtttgg
    40021 gcaacgaatt gggtataaat caaccctctc tccgagccac cagcaattaa tgctttccac
    40081 tcttcagggg aagagcgatg ccatgtcaga tataagccag ctaaatcatt ggcatgacca
    40141 tcatcagtca tctgataaat agtctgaatt aaatcatcac gattatttat tccccaggat
    40201 gatgagagat cgaattcttt tatcccggta tacatttttg gcgagtcatg cgcgggtttg
    40261 caaccctgat taagtatttc catgggcgca gaaagcgcaa aaagaagata ttgttcttcc
    40321 ttattca
//
LOCUS       DAZGJW010000026        39946 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZGJW010000026 DAZGJW010000000
VERSION     DAZGJW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.026"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..292)
                     /gene="chbB"
                     /locus_tag="ACHKIK_001432"
     CDS             complement(<1..292)
                     /gene="chbB"
                     /locus_tag="ACHKIK_001432"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO9082632.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAV"
     gene            complement(591..929)
                     /gene="osmE"
                     /locus_tag="ACHKIK_001433"
     CDS             complement(591..929)
                     /gene="osmE"
                     /locus_tag="ACHKIK_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO9082633.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1131..1958
                     /gene="nadE"
                     /locus_tag="ACHKIK_001434"
     CDS             1131..1958
                     /gene="nadE"
                     /locus_tag="ACHKIK_001434"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO9082634.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2188..3075
                     /gene="cho"
                     /locus_tag="ACHKIK_001435"
     CDS             2188..3075
                     /gene="cho"
                     /locus_tag="ACHKIK_001435"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO9082635.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3035..3610)
                     /gene="ves"
                     /locus_tag="ACHKIK_001436"
     CDS             complement(3035..3610)
                     /gene="ves"
                     /locus_tag="ACHKIK_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO9082636.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3813..4298)
                     /gene="spy"
                     /locus_tag="ACHKIK_001437"
     CDS             complement(3813..4298)
                     /gene="spy"
                     /locus_tag="ACHKIK_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO9082637.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPIMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIVASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4628..5596)
                     /gene="astE"
                     /locus_tag="ACHKIK_001438"
     CDS             complement(4628..5596)
                     /gene="astE"
                     /locus_tag="ACHKIK_001438"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO9082638.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5589..6932)
                     /gene="astB"
                     /locus_tag="ACHKIK_001439"
     CDS             complement(5589..6932)
                     /gene="astB"
                     /locus_tag="ACHKIK_001439"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO9082639.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6929..8407)
                     /gene="astD"
                     /locus_tag="ACHKIK_001440"
     CDS             complement(6929..8407)
                     /gene="astD"
                     /locus_tag="ACHKIK_001440"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO9082640.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8404..9438)
                     /gene="astA"
                     /locus_tag="ACHKIK_001441"
     CDS             complement(8404..9438)
                     /gene="astA"
                     /locus_tag="ACHKIK_001441"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO9082641.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9435..10655)
                     /gene="astC"
                     /locus_tag="ACHKIK_001442"
     CDS             complement(9435..10655)
                     /gene="astC"
                     /locus_tag="ACHKIK_001442"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO9082642.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQLGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            11101..11907
                     /gene="xthA"
                     /locus_tag="ACHKIK_001443"
     CDS             11101..11907
                     /gene="xthA"
                     /locus_tag="ACHKIK_001443"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO9082643.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12074..12784
                     /gene="ydjX"
                     /locus_tag="ACHKIK_001444"
     CDS             12074..12784
                     /gene="ydjX"
                     /locus_tag="ACHKIK_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO9082644.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLLGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            12789..13466
                     /gene="ydjY"
                     /locus_tag="ACHKIK_001445"
     CDS             12789..13466
                     /gene="ydjY"
                     /locus_tag="ACHKIK_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO9082645.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            13481..14188
                     /gene="ydjZ"
                     /locus_tag="ACHKIK_001446"
     CDS             13481..14188
                     /gene="ydjZ"
                     /locus_tag="ACHKIK_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO9082646.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            14188..14736
                     /gene="ynjA"
                     /locus_tag="ACHKIK_001447"
     CDS             14188..14736
                     /gene="ynjA"
                     /locus_tag="ACHKIK_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO9082647.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14746..15912
                     /gene="ynjB"
                     /locus_tag="ACHKIK_001448"
     CDS             14746..15912
                     /gene="ynjB"
                     /locus_tag="ACHKIK_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO9082648.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            15885..17420
                     /gene="ynjC"
                     /locus_tag="ACHKIK_001449"
     CDS             15885..17420
                     /gene="ynjC"
                     /locus_tag="ACHKIK_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO9082649.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPTLLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17420..18073
                     /gene="ynjD"
                     /locus_tag="ACHKIK_001450"
     CDS             17420..18073
                     /gene="ynjD"
                     /locus_tag="ACHKIK_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO9082650.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMEQWSENYNKLR"
     gene            18140..19447
                     /gene="ynjE"
                     /locus_tag="ACHKIK_001451"
     CDS             18140..19447
                     /gene="ynjE"
                     /locus_tag="ACHKIK_001451"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO9082651.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(19456..20076)
                     /gene="ynjF"
                     /locus_tag="ACHKIK_001452"
     CDS             complement(19456..20076)
                     /gene="ynjF"
                     /locus_tag="ACHKIK_001452"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO9082652.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            20163..20570
                     /gene="nudG"
                     /locus_tag="ACHKIK_001453"
     CDS             20163..20570
                     /gene="nudG"
                     /locus_tag="ACHKIK_001453"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO9082653.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(20536..20808)
                     /gene="ynjH"
                     /locus_tag="ACHKIK_001454"
     CDS             complement(20536..20808)
                     /gene="ynjH"
                     /locus_tag="ACHKIK_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO9082654.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            21044..22387
                     /gene="gdhA"
                     /locus_tag="ACHKIK_001455"
     CDS             21044..22387
                     /gene="gdhA"
                     /locus_tag="ACHKIK_001455"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO9082655.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLERQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(22504..23544)
                     /gene="ynjI"
                     /locus_tag="ACHKIK_001456"
     CDS             complement(22504..23544)
                     /gene="ynjI"
                     /locus_tag="ACHKIK_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO9082656.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEYFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(23672..25633)
                     /gene="topB"
                     /locus_tag="ACHKIK_001457"
     CDS             complement(23672..25633)
                     /gene="topB"
                     /locus_tag="ACHKIK_001457"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO9082657.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQSVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(25638..26681)
                     /gene="selD"
                     /locus_tag="ACHKIK_001458"
     CDS             complement(25638..26681)
                     /gene="selD"
                     /locus_tag="ACHKIK_001458"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO9082658.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIH"
     gene            complement(26798..27349)
                     /gene="ydjA"
                     /locus_tag="ACHKIK_001459"
     CDS             complement(26798..27349)
                     /gene="ydjA"
                     /locus_tag="ACHKIK_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO9082659.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            27510..29366
                     /gene="sppA"
                     /locus_tag="ACHKIK_001460"
     CDS             27510..29366
                     /gene="sppA"
                     /locus_tag="ACHKIK_001460"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO9082660.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            29533..30549
                     /gene="ansA"
                     /locus_tag="ACHKIK_001461"
     CDS             29533..30549
                     /gene="ansA"
                     /locus_tag="ACHKIK_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO9082661.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            30560..31201
                     /gene="pncA"
                     /locus_tag="ACHKIK_001462"
     CDS             30560..31201
                     /gene="pncA"
                     /locus_tag="ACHKIK_001462"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO9082662.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(31362..31634)
                     /gene="yeaC"
                     /locus_tag="ACHKIK_001463"
     CDS             complement(31362..31634)
                     /gene="yeaC"
                     /locus_tag="ACHKIK_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO9082663.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(31676..32089)
                     /gene="msrB"
                     /locus_tag="ACHKIK_001464"
     CDS             complement(31676..32089)
                     /gene="msrB"
                     /locus_tag="ACHKIK_001464"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO9082664.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            32431..33426
                     /gene="gapA"
                     /locus_tag="ACHKIK_001465"
     CDS             32431..33426
                     /gene="gapA"
                     /locus_tag="ACHKIK_001465"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO9082665.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            33510..34394
                     /gene="yeaD"
                     /locus_tag="ACHKIK_001466"
     CDS             33510..34394
                     /gene="yeaD"
                     /locus_tag="ACHKIK_001466"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO9082666.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(34445..35299)
                     /gene="yeaE"
                     /locus_tag="ACHKIK_001467"
     CDS             complement(34445..35299)
                     /gene="yeaE"
                     /locus_tag="ACHKIK_001467"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO9082667.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(35389..36135)
                     /gene="mipA"
                     /locus_tag="ACHKIK_001468"
     CDS             complement(35389..36135)
                     /gene="mipA"
                     /locus_tag="ACHKIK_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO9082668.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            36571..38505
                     /gene="yeaG"
                     /locus_tag="ACHKIK_001469"
     CDS             36571..38505
                     /gene="yeaG"
                     /locus_tag="ACHKIK_001469"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO9082669.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            38618..39901
                     /gene="yeaH"
                     /locus_tag="ACHKIK_001470"
     CDS             38618..39901
                     /gene="yeaH"
                     /locus_tag="ACHKIK_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO9082670.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         9666 a        10485 c        10042 g         9753 t
ORIGIN      
        1 caaccgcagc cttaagcacg cctaaaccat cgactttgcc ataaagcagc gagtcaatta
       61 cttcaaccgg tttgttgggt aacaaacgct ggatttcggg caacatataa gcaatctgcg
      121 gccctaataa cacgacatcg gcattctgac ctttttcacc agccagtgtt tccggaaatg
      181 cttcaataat gaccggaact tcatattttt ctgcctgtgc gcgcattttt gataccagta
      241 aagaggtaga catgcccgca gaacaaaaca gataaatgtg tttcttttcc ataaaactgc
      301 cctcatcgac gattatctgt cagccagaca ctccgcaagc cttaacctgc ttccatgctc
      361 tgggtaactt gcgaaaccaa catgactttt tttgtggctg aaaggagtat acggtataga
      421 cctgagcaat agtatttctt tgaccttata aattgtttct ccttgacctg tggttatgac
      481 cctcttcaca tttcgggcaa ataattcgcg aagataatta agaactcagg ccaaaaaaaa
      541 ccggcgcaat ggccggtttc cgttgttact caagctttca gacgaattga ttacttcgca
      601 gcctgtggat cagtgtcgta ttcagcacag gtctgataac cggagttgat gacatgtccg
      661 gtatcatcta acgcgacaaa gtaggtttct gctttaccat cacgttgacc caggatgtag
      721 gtctggcaag taccgcgagc atggatcatg ctcacttcag acgaaggttt acccgcaatt
      781 tgtgcaacct gcgcccggct catgcctttt ttgacgtctt tcaccacagg ctgtacaaac
      841 tggtctttgg tacgatcata agccgtacaa cccgccagca tggttaatac cgccgctgca
      901 ctcagaattc ctgccatatt cttgttcata ttccgtcctc ttgtttatca gcgtgttaga
      961 taagcctgga atacattggg cgctttttca agcccgtgaa cgaaacggct ccgctttcag
     1021 aggattcctg tatgacgttt taaccaccat tcagcccgct gtcgcttgtc gtttcagtag
     1081 caacgggtta gctttaagga agttttgtct tttctgtctg gaggggttca atgacattgc
     1141 aacaacaaat aataaaggcg ctgggcgcaa aaccgcagat taatgctgaa gaggaaattc
     1201 gtcgtagtgt cgattttctg aaaagctacc tgcaaactta tccgttcatt aaatcactgg
     1261 tgctcgggat cagcggcggt caggactcca cgcttgccgg aaagctgtgc cagatggcga
     1321 ttaatgagct gcgccaggaa actggcaacg aatcactgca atttattgcc gtacgcctgc
     1381 cctatggtgt tcaggctgac gaacaagatt gccaggatgc cattgccttt attcaaccgg
     1441 atcgcgtatt aaccgttaat atcaagggcg cggtattggc tagcgagcag gcattgcggg
     1501 aagcaggcat tgaactgagc gattttgtcc gtggcaatga aaaagcgcgt gagcggatga
     1561 aagcacaata tagcattgcg ggtatgacca gcggtgtcgt ggtgggcacc gatcatgcag
     1621 cagaagccat taccggattc ttcactaaat atggtgacgg cggtacggat attaatccgc
     1681 tgtatcgtct caacaaacgc cagggtaaac agttgctgac ggcattaggt tgcccggaac
     1741 acctttataa gaaagcccca acggccgatc tggaagatga tcgcccttct ctgccagatg
     1801 aagtggcact cggcgtgacc tatgacaata tcgacgacta tctggaaggg aaaaacgtac
     1861 ctgaacaggt cgccagaaca atagagaact ggtatctgaa aaccgaacat aaacgccgtc
     1921 cgccaattac cgttttcgat gatttctgga aaaagtaata atttgcacat aatggtttgt
     1981 gcgaaaaaga gtaatttgtt cacgccggat gcggcgtgaa cgccttattc gacctacaaa
     2041 actatgcaaa ttcaatatat tgcaggagtc gaataggcct gacaggcgta gcacgtcaga
     2101 cggtgtaacc tttgtcatcg acccgcctct tttttaatcg cttcccgcct gttacactgg
     2161 atagataacc agcattcgga gtcaacagtg gtacggcgtt taacttctcc gcggctcgaa
     2221 tttgaagctg cggcaattta tgaatatccc gaacatttac gttcattcct taatgactta
     2281 cccacccgac caggggtgta tctgtttcat ggcgaaagtg acaccatgcc gctctatatc
     2341 ggcaaaagcg ttaatatccg cagtcgggtg ctttctcatt tacgcacccc ggatgaagcc
     2401 gccatgctac ggcaatcccg gcgaattagc tggatctgca ccgcaggcga aatcggcgct
     2461 ctgctccttg aagcgcgatt aatcaaagaa caacagccgc tgtttaataa acggttgcgc
     2521 cgcaatcgcc agctctgtgc cctgcaatta aatgaaaagc gcgtcgatgt ggtgtatgcc
     2581 aaagaggtgg atttttcacg agcccccaac ctgtttggcc tgtttgccaa taggcgcgca
     2641 gctttgcaag cattgcagag catcgctgat gaacaaaaac tttgttatgg cctgctggga
     2701 ctggaaccgt taagtcgcgg tcgtgcatgt tttcgttcag cgctaaaacg ttgcgccgga
     2761 gcatgctgcg gtaaagagag ccatgaggaa catgcgctac gcttgcgcca gtctctggag
     2821 cgtttgcggg tggtgtgttg gccttggcaa ggggcggtgg cgctgaaaga acagcacccg
     2881 gaaatgactc aatatcatat tattcaaaac tggctgtggc tgggggcggt taattcgctg
     2941 gaagacgcga caacgttaat tcggacaccc gccgggtttg atcacgacgg ttataaaatt
     3001 ctttgtaagc cgctgctttc cggtaactat gaaattactg aacttgatcc ggcgaatgac
     3061 cagcgagcca gttgatttca ctgaacaaca gtttaccttg cggttgtaaa agccgcaggg
     3121 tgtgcctgcc atcgaaccag cacgcgccct ggtcggtagt gagcagtttg tcgcccagtt
     3181 gccacgcccc gttaatcaca aacactaccc cgccgcgtga gccaaaggta gtaaacgtac
     3241 gttcggcaat tctgactttc gccttacaga catccaggcg cgtcataata ttgaaatcca
     3301 tcgacatctg ccctgccgtc agtttcgctt ttaccacctg gtccgccgca aaggcaaaag
     3361 gctgtaacgg ttttaaggta tggttaaagt ggtctgcgct ttcaaggaac atctcgccgc
     3421 cttccagcaa cgtcactatc ctttccatac cgggaaacaa agaaaactcc ccattcgccg
     3481 cgatggatgc aatgctggca cgccagtaaa aatcacgttt tgccggtggg aaagtgcaaa
     3541 tttcgcgcgt ttcaccggca gcatttcgcc acagattcac cgacatttta cgcatatcaa
     3601 agtattccat actcgctccc ggcccgttct tttcattgtt atcgttcagg taaccgtgga
     3661 tggttatcgg caactcgggt gatttacgtt agtggtgatc aggctttttt tacaactgtt
     3721 tgagaagaga aaagaaaacc gccgatcctg tccaccgcat tactgcaagg tagtggacaa
     3781 gaccggcggt cttaagtttt ttggctgaaa gattattcag cagttgcagg cattttacct
     3841 tttgccgctg gacgttctgt cagacgcttc tcaaaattag cattaaattg ctttttctgt
     3901 tccggcgtca ggatgttgta aattttgttc tgggtttcca tgtgcgccag catgttagct
     3961 ttgcgctgtt cttccatttt tgcgatctgc gcttcagctt ttgctttatc gaaggtatcg
     4021 ctggcaacga tgtcatgcat tgcgcggcgt tcttccagcg gcggacgttt catctggtca
     4081 cgctggcctt tcatgatttc gcggatctgc tgtttctgcg cgtcggtcag gttcaggtct
     4141 ttgaacatca tgtcctgatg cggaccgaac ttgcctttgt ggtgcataat cggcttcgcg
     4201 tcagccggtg ctgcggtagt ggtgtctgcg gcatgggcca ggttagccgc gccaagagcc
     4261 agggtagagg caacaaacag tgcagttaat ttacgcatat tctatatcct tcctttcagt
     4321 tatttattac ggctttctta agtagcgtgc cgtgttgacg agattaactt tactgacttt
     4381 agcgtcaatt aatcagagca acggtaaaac aatgaaagtg taaaaaacac ttttgcgcca
     4441 attatggaga aaaaaagaaa atttgatgga gagtgatgag agaatattac aacacgatga
     4501 ttttgcagag attatgaaga actataccgg atgactggtg ataaataaag caaataacca
     4561 ggattaatct gtattaattt ataagaaagc aacttaatac ccgcagagtg atttctgcgg
     4621 gtaagtatta gcttattttt tcgagcatta atcccgcgcg taatcccaac gctaccaacg
     4681 gattagggaa taacacatac tctacatcat gggttacggt aaaacgttcc tctccgtcct
     4741 gcgccagcaa tgttcctttc tcaaacggca taaaattcag cgtgtcactt gccatatgca
     4801 tttcgaagga cggcgagtgg cgagtaattt gcgaaaccac ccgataacgg agcggcggtg
     4861 ttctcacgat accgacactc tcaccagata gcagcgcagc aattgcgctg gcagttactg
     4921 caaactggcg aagatcgttt tgcccaaagg gcaacgcttt gccaagttcc agcgtacagg
     4981 ccagcgcgcc aaaatgtctg gcgctgaaat gggtaaacgt accaccaggt tcctgatgga
     5041 acaccagcgc ctccagcccc gccgcaccca gccacgtcag aaatttctcg tcccagggaa
     5101 tgtcgcgttg cggtaataca ccgaactgcg gatgcaagga gccacgaatt gcggtatgta
     5161 gatcaaggtg ccagcgcaca gattctttgc cctggtcata aaaatcttcc aggcactgtt
     5221 ccagttcgcg cgcccgacag gtttctccgc tttcagcaaa tagctgccaa cgaccgccaa
     5281 acattcgatt catatcgcta tggcaataac gtttcccttg cttcagcgca ggaggattcc
     5341 cgaggatcac cagcaaccgc caacgtaacg ggatctcgcc gtgagatatc gcgccaagca
     5401 acgcgtccag catctccaca ggtgccgtct cattaccgtg tattcccgct gaaatcacca
     5461 gtgcgccttg cggtggcgtt aatggcgtca gttccagcac accatcgccc agccagcgcc
     5521 agcgaacgcc gttgatttct cgctcggtga taaccggttt tttacccgtt aaggtcagag
     5581 caagaaaatt atccattgcc ccctccctcg cgctggaacg gataaaccga accgagattc
     5641 agtaattggc tcaatacatc cagtgcttcc cgcccttcgc gcagcaattg cgggtcggcc
     5701 agatcggcag cagtaaggcg atcgcggtag taacgatcca cccagtcatt gagcgcatta
     5761 aacagcgtat cgttcatcat caccgccgga ttcaccgccc ggcgttcttc ttctgtcaat
     5821 accacccgca accgcaggca cgccgggccg ccgccattcg ccatgctttc acgtaaatca
     5881 aagactttta gttcgctaat cgggttgtca gcggcaagga gttcattgag ataaccccat
     5941 actccggcgt gttcccgaca ctcctgaggc agcaccaaca tcatagaacc atcatcgcgg
     6001 ctcaacagtt ggctgttaaa cagataggta gacaccgcat ccgacacgga aacctgagtt
     6061 gccggaactt ctatcgccat aaaaccgttc acccgcgcac gcaggtttgc cagtaactgt
     6121 gactggcgag cgaacgcctg ttggtggcaa aacagcacct ggcggttact cacggcaatc
     6181 acgtcattat gaaaaacgcc ctggtcgata acgtccgggt tttgctgggc gaaaatcacc
     6241 tgttggggat tcacctgatt cagccttgcc accgcctcgc tggcttcgcg agtctgtcgc
     6301 gccggataac gggaaggccg ggtatcattg ccttcttctc gcccgtagac aaaaagttgc
     6361 atacccggct caccgtaatg accgccgaga cgattgtggt ttgccgcccc ctcatcaccg
     6421 agcaacgcta cctgcggcaa cgccgaatga acgctaaatt tctcttcgtc gttaaaaatc
     6481 gcttttaaaa gtgattcggt gacgtgcgct tccagcgagc ggtgaaattt attgttcagg
     6541 ttggcaacgg tgagatgcac tttgccatcc agcgtatcgg cagatggcgc gatcgttgcc
     6601 gcattggcta cccacattgg cgaagcggaa ctgacgctgg aaagccagtg cggtgcctgg
     6661 cgtgcaactt tttccagtac ctgctcatcg ctaccactga atcccaactg acgcagcacc
     6721 ggaataaacg gacgctcgtg cggcgggatc acggcctggg ggaatcccgc atcggcaagg
     6781 gctttcattt tcagtaagcc ctgttttgcc gcctgtcgcg ggttagacac ctgaaaacgg
     6841 tgacgggtag aggcttcatt accaaacgac aggccagcgt aatgatgcgt cagccctacc
     6901 agcccgtcga aattaacttc ccaggcgttc atcgcaccac ctcatcggaa aaatccagcc
     6961 cggggttaag cgttgcgggc aaagttaacg agtccgactc caggctcgcc ataggccatg
     7021 cgcagtaatc tgcggcatac caggcgctgg ggcgatggtt accggatgca ccaatgccgc
     7081 cgaatggcgc ggtactggca gcaccagtaa gcggtttgtt ccagttaaca atccccgccc
     7141 gcgcctccag caacagttga tcgaattttt cccgctcggg ggaaaccaga ccgcaagaga
     7201 gtccgaagcg agtgttattc gccattagaa tcgcttcctc gaaagaatca taacgccaga
     7261 cgcgcaataa cggtccgaac acctcttcat ctggtacgcc agcaacgcct gtcatttcaa
     7321 tgatccccgg cgtcagcaac gatgtctctg attgtaataa gcgcggcgca agcagggttc
     7381 gtccgcccat cgcttccagt tgctgccagg cagtaaccac ctgctgtgcg gcctgttcag
     7441 aaatcagccc gccaataaac ggctgcggtt cgtcatccca gttgcccggc gttaatcgct
     7501 ggctgacggc aaccagacga gcaagaaacg catcgccctg cgctccgctt ttgagcaata
     7561 aacggcgggc gcaggtgcag cgttgcccgg ctgtgacaaa cgccgactga atggtcagat
     7621 ggacagccgc gtcgatatcc gccacctcat cgataattag cggattatta ccgcccatct
     7681 caagggcgag aattttctcc ggctgaccgg agagctggcg atgcagctgg tagccggtat
     7741 tggcgctacc ggtaaacagc aaaccgtcga gatcctccag cgcactcagc gcctgacccg
     7801 tttcacgccc gccctgcacc aggttcagta cgcccggcgg caagccagcc tgctgccata
     7861 aacgcattac cgcttcgcca ctccacggtg tcagttcgct gggtttaaag ataatggtgt
     7921 tacctgccag caatgccgga acgatatgtc cgttcggcaa atgaccaggg aaattatacg
     7981 gcccaaacac cgccagcacg ccgtgcgggc gatgtcgcag gctcgccgca ccgtccggca
     8041 tttcactacg ctgctcgccg gtacgaacgt gatacgcctt aattgatatc gcgattttat
     8101 tgatcatcgc cgtcacttcg gttgccgctt cccagcgcgg cttacccgtt tctctggcaa
     8161 taatcgcggt taattcgcct ttattccttt ccagcaatcc ggcaaagcgt tcgacaacga
     8221 cctgacgttc agccaatgag agccgcgccc agcgcggaaa cgccgcacgg gctgcccgac
     8281 aagcctgttc gacctgagcg gcatcggcat cattgccttg ccataacacc tcgcccgata
     8341 ccggattacg cttcacacgc gatgcgccct ggcccgttat ccagtcaccg ttaatccata
     8401 aagtcatgct gttttctcct ctgcgcacag gcgcaccaga cgaacgcgat cccctgcgtg
     8461 gcatttgagg gcatccagtt gtgcggcggt taaaatcaaa cgctcggttg ccggatcggt
     8521 acgcgccagc accacgcgga aatggtgata attttcattg gcgaccaggc aggccggaaa
     8581 atcgccctgc gcaggctgcc cttctgccac ttccaccagc cgacttttac gaatggcgcg
     8641 cacgcggtcg atgtcacact caagcgttgg cccgccgtcg aagatgtcga tatagttgcg
     8701 gtaacgaaaa ccttctttct ccagcaccgc gcgggcaggc gcggtttgcg gatgtacctg
     8761 accgatgacg tcctgggctt cctgggataa aaagtgggta tagatcggat gtttcggcat
     8821 cagttctgca ataaatgcct tttgcccggt gccgcagaga aaatcggcgc ggctaaaatc
     8881 catcgaaaag aagcgtttac cgaggctttg ccagaacggt gaatagccgt gttcgtcaat
     8941 caccccgcgc atttcagcaa ccactttgtc attaaacttg tcgcgaaaag ccgccataaa
     9001 cataaagcgc gatttcgaca gcaaatagcc gttgccctct ttgcgccagt ccgggtcgag
     9061 aaacagcgtg cacagctcgc tgctgccggt gtgatcgtta ctgagaaaca gcgtcggcaa
     9121 tgcgttatag acattcagct cttttgaggc gtgaaccaac gtgccgacgc gatagttgta
     9181 ccagggatcg ttcagcccaa ccgccacctc aatggcacaa atccccgcca cggtgcctgt
     9241 ctcgctatct tccagcacga acacatagcc ctgctcactt ttgggcagtt cgccttgcca
     9301 ggttttgatt gccctttcga tacgcgccga aagcgtggct tcattggcgg gaagcgacgt
     9361 caggccgccg cccgttttgc tggcaagctg catcagcgcc gagacatctg atcgctcaac
     9421 gggacggatg accatcatga tgaacctcgg ctaacaaagt gttcgcaagc agctgcaaag
     9481 cgatccagtc cggtcgtcac ctcttcttcg ctgacattga gcgcaggcgc aaaacgcacc
     9541 acgttgccac ccgcaatcag taccatcacg cctgctttcg ccgcttcctg agagatctgt
     9601 ttcgcttgcc cggcgtaatc ggcattcagt acacagccaa tcagcaaacc taagccgcga
     9661 acttcactga acaaaccata gcggtgatta atagtattaa gacgctcaac aaaccagtcg
     9721 tgacgctgtt taacgccatt aagcatctct ggtgtgttga tgagctccag cactttgcct
     9781 gccaccgccg aggccagcgg attaccgcca taggtggtgc catgagtgcc aacggtcatc
     9841 acgcgggcgc actcttcggt tgccaacaac gcaccgaccg ggaaaccgcc gcccagcgct
     9901 ttggcggtag ttaacagatc aggcgtcacg ccgtagtgca tataggcata cagttccccg
     9961 gtgcgcccga cgccggtttg tacttcatca aaaatcaaca gcgcattgtg gcggttacac
    10021 aattcacgca gaccttgtaa aaacgcgttg ctggctggca ccacaccgcc ttccccctgg
    10081 atgggttcga caatcaccgc acaggtagag tcgtcaatca gcgcgctggc agagttaata
    10141 tcgttatatg cagcatgacg aatatccgcc ggcagtggcg caaaatcctg tgaataggct
    10201 ggctgcccac ccgcactgac agtaaacagc gtgcgaccat gaaacgcatt tttgaacgcc
    10261 acgatgccgc tcttatggct gccgtagcgg tcgtgagcga atttacgcgc cagttttagc
    10321 gccgcttcgt tggcttccgc accggagtta caaaagaaga cgcgatcggc aaacgtggcg
    10381 tcgatcaatt ttttcgccag tcgcagtacc ggctcgttgg tgtaaccgtt gccggtatgc
    10441 cagaacttac tcgcctgttc gttcagcgct tcacgcagtt ccggatgcgc atggcccagc
    10501 gcgttcaccg caatgccacc cgcgaagtcg atatactctt tccccagctg atcccacaag
    10561 cgcgaacctt cgccacgtac cggtataaag ggtgccggag cgtaaacagg tatcatccat
    10621 tcatcaaagt tttcacgcgt aattggctga gacatagcga cctctacagt aaataattcg
    10681 ttatttatat gttaacaata agtaatgttt gcgctgtaaa tgtagattgc agggttcgtg
    10741 ccagccagtg ataaaagtgc ataaacggcg gaggctaact ggaaatcaag gagttataac
    10801 caaaccatat gcatttaaag tgcatataaa gtgaatacgt ttgcgatgtg ggtgaataaa
    10861 aagaataaaa aacgcaatgt tatgcagaag taaaatataa ttctggaatt gtgatcattg
    10921 acgaaattta ctggaaatta ctgcgccatt ctgacgcagc gcgcaccaaa agcgggcatt
    10981 ttttgcgcca tcgttgacat cattaacaac catcgatcaa atcacttaac aacaggcggt
    11041 aagcaacgcg aaattctgct accatccacg cactctttat ctgaataaat ggcagcgact
    11101 atgaaatttg tctcttttaa tatcaacggc ctgcgcgcca gacctcacca gcttgaagcc
    11161 atcgtcgaaa agcaccaacc ggatgtgatt ggcctgcagg agacaaaagt tcatgacgat
    11221 atgtttccgc tcgaagaggt ggcgaagctc ggctacaacg tgttttatca cgggcagaaa
    11281 ggccattatg gcgtggcgct gctgaccaaa gagacgccga ttgccgtgcg tcgcggcttt
    11341 cccggtgacg acgaagaggc gcagcggcgg attattatgg cggaaatccc ctcactgctg
    11401 ggtaatgtca ccgtgatcaa cggttacttc ccgcagggtg aaagccgcga ccatccgata
    11461 aaattcccgg caaaagcgca gttttatcag aatctgcaaa actacctgga aaccgaactc
    11521 aaacgtgata atccggtact gattatgggc gatatgaata tcagccctac agatctggat
    11581 atcggcattg gcgaagaaaa ccgtaagcgc tggctgcgta ccggtaaatg ctctttcctg
    11641 ccggaagagc gcgaatggat ggacaggctg atgagctggg ggttggtcga taccttccgc
    11701 catgctaatc cgcaaacagc agatcgtttc tcatggtttg attaccgctc aaaaggtttt
    11761 gacgataacc gtggtctgcg catcgacctg ctgctcgcca gccaaccgct ggcagaatgt
    11821 tgcgtagaaa ccggcatcga ctatgaaatt cgcagcatgg aaaaaccgtc cgatcacgcc
    11881 cccgtctggg cgaccttccg ccgctaattt agcagctctc ctggctcaaa ctgggtcagg
    11941 agaattaacc ttgagaaaaa tcaacaaact gtcagtaatg atttgttgcc tgccgtcctt
    12001 tgttataccg tcactgcgtt tttagttgtc tgaccacttc tctattatca agtttgatat
    12061 aggaaactcc acgatgaacg ctgagcgtaa ctttctgttt gcctgtctta tttttgcgct
    12121 ggtcatttac gctatccacg ctttcggttt attcgatctg ctcaccgatt taccccactt
    12181 acagacactc attcgccaga gcggattgtt cggttatagc ctctatattc tgttattcat
    12241 cattgccacc ctctttctgt taccaggaag catactggtg atcgctggag gaatagtttt
    12301 tggcccgctc ttagggacac tactctcatt aattgccgca acgctggcct cctcgtgctc
    12361 attcctgctg gcgcgctggc tgggacgtga tttactgctg aaatacgtgg gccatagcca
    12421 taccttccag gccattgaaa aaggcattgc gcgtaacggt attgattttc ttattctgac
    12481 ccgcttaatc ccgttgtttc cttacaatat tcaaaattac gcttacggat taaccaccat
    12541 cgccttctgg ccttataccc ttatttcggc actcacgacc ctgctcggta ttgttattta
    12601 taccgtgatg gcaagcgatc tcgccaatga aggcattacg ctgcgcttta ttttacaact
    12661 ctgtctggcg ggcctggcgc tgtttattct cgtccagctc gcaaaactct acgcccgaca
    12721 caaacatgtg gatctgtctg cttcgcgccg cagcccactt actcacccaa aaaatgaagg
    12781 atagaacgat gtcgcaacat tattcagtgt catggaaaaa aggactggct gcactctgct
    12841 tactggctgt tgccgggctt agcggctgcg atcaaaaaga gaatgcggcg gcaaaagtgg
    12901 aatacgacgg actttcgaac agccaaccgc tgcgcgtcga tgccaataac catacggtaa
    12961 ccatgctggt gcaaattaat ggtcgtttcc tcaccgacga cactcgtcac ggtattgtgt
    13021 ttaaagatgg ctccaacgga cataaatcgc tgtttatggc ttatgcgacc ccgaaagcat
    13081 tttatgaagc cctgaaagag gcaggtggta cgccgggcga aaacatgacg atggataata
    13141 aagaaacgac tcatgtcaca ggcagcaaac tggatatttc ggtcaactgg caaggggcgg
    13201 caaaagcgta ttccttcgat gaagtgattg ttgacagtaa tggcaagaaa ctggacatgc
    13261 gctttggcgg taatttaacg gcagcagaag agaagaaaac gggttgcctg gtgtgtctgg
    13321 atagctgccc ggtcggcatc gtcagcaatg caacatacac ttatggtgcg gtggaaaaac
    13381 gcggcgaagt gaaattcaaa ggcaatgcct cagttctccc ggcggataac acgctggcaa
    13441 cggttacctt taaaatcacc gaataaagcc aggataaagg atgatgaaga tacaatcgcg
    13501 taaaatctgg tactaccgta taaccctcat catcctgttg ttcgctatgc tgctcgcatg
    13561 ggcgctgctt cccggcgtcc atgagtttat caatcgcagc gttgcggcgt ttgccgccgt
    13621 ggaccaacag ggtatagaac gctttattca gtcttacggc gcactggcgg cagttgtctc
    13681 gttcttgttg atgattttac aggccattgc tgcaccgcta cctgcgtttt tgatcacctt
    13741 tgccaatgcg tcgctgtttg gcgcgttctg gggcggcttg ctgtcgtgga ccagttcgat
    13801 ggccggcgcg gcgctgtgct tttttatcgc cagagtgatg ggccgcgaag tggtggaaaa
    13861 attaaccggc aaaaccgtgc ttgacagtat ggacggcttt ttcactcgct acggcaaaca
    13921 caccatcctg gtctgtcggt tattgccttt tgtccctttc gatccaatca gctatgctgc
    13981 cggtctgact tcaatacgtt ttcgctcgtt ttttatcgcc accgggcttg gtcaattacc
    14041 ggcgactatt gtttattcct gggcgggcag catgttaaca ggcggtactt tctggtttgt
    14101 caccggactg tttattctgt ttgccctgac cgtggtgatt tttatggcga agaaaatatg
    14161 gcttgagcgc cagaagagga atgcctgatg ggtttaccgc cgcttagcaa aattcctttc
    14221 attttacgtc cgcaggcgtg gctgcatcgt cgccattacg gcgaggtgct aagcccgatt
    14281 cgctggtggg ggcggatccc gtttatcttt tatttggtgt caatgtttgt tgggtggctg
    14341 gagcgcaaac gctcaccgct cgatccggta gtgcgatcgc ttgtcagcgc gcgcattgcg
    14401 caaatgtgcc tgtgtgagtt ttgcgtggat atcaccagta tgaaagtcgc cgagcgtacc
    14461 ggcagcaccg ataaactgct ggcagtggct gactggcggc aaagcccgct cttcagcgat
    14521 gaagaacggc tggcgctgga gtacgccgaa gccgcaagcg taacgccgcc aacggtcgat
    14581 gatgccctgc gcacccgact ggctgcgcat tttgacgctc aggcgctcac cgaactgacg
    14641 gcattgatcg gcctgcaaaa cctgtcggct cgttttaatt ctgccatgga cattcccgct
    14701 caggggctgt gccgtattcc ggaaaaacgt tcttaaggag agaagatgcg ccattgtggg
    14761 tggttgctgg gattgttatc gctgttttct ctggcaacac atgccagtga ctggcaggaa
    14821 attaaaaatg aggccaaagg gcaaaccgtc tggtttaacg cctggggcgg cgataccgca
    14881 attaaccgct atctcgactg ggttagcggc gagatgaaaa cccattacgc tataaacctg
    14941 aagattgttc gtctggcgga tgccgcagac gcggtgaagc gcattcagac cgaagctgct
    15001 gccggacgta aaacgggcgg ctcggtggat ctgctctggg tgaacggcga aaacttccgc
    15061 accttaaaag aggccaattt attacaaacg ggctgggcgg agactctgcc caactggcgc
    15121 tatgtcgaca cacagctgcc ggtgcgggaa gatttttcag tgcctacaga aggggctgaa
    15181 tcgccctggg gcggcgcaca actgacgttt atcgcccgcc gcgatgttac gccacagcca
    15241 ccacaaacgc cgcaagcctt actggagttt gctaaagcca atcccggcac ggttacctat
    15301 ccgcgcccac cggactttac cggcacggcg tttcttgaac agttgctgat tatgctgacg
    15361 cccgatcccg ccgcgttaaa agttgccccg gataaagcca ctttcgcccg cgtcactgcg
    15421 cccttgtggc aatatcttga cgcgctacat ccctatttat ggcgcaaagg aaaggatttc
    15481 ccgccttcgc ccgcgcggat ggatgctctg ctgaaagccg gcacattgcg cctgtcgctg
    15541 acctttaacc ctgctcatgc ccagcaaaaa atcgccagcg gcgatttgcc tgcaagcagt
    15601 tacagttttg gttttaacca ggggatgatt ggcaacgtgc atttcgtcac cattcctgct
    15661 aacgcaaatg ccagtgctgc ggcgaaggtg gttgccaact tcctgctctc acccaatgcg
    15721 caactgcgta aagcggatcc cgctgtctgg ggcgatcctt ctgttctcga tccgcaaaaa
    15781 ctgcctgatg ggcagcgcga aacattgcaa tcaagaatgc cgcaagatct gccgccggta
    15841 ctggctgaac cgcacgcagg ttgggtaaat gcgctggaac aagaatggct acaccgttac
    15901 ggtacgcatt aatctttttg ctgtgggcga tggtggcggt gatttatgca ccactgatcc
    15961 cggcagctct cacgctgata tcgcctgctt tgtcgttgac acactggcag gcgttatttg
    16021 ccgatccaca gttaccgcag gcattactgg caacgctggt gtcgacaacc atcgcggcgg
    16081 tcggggcgtt gttgattgcc ctgctggtga ttgtggcgct gtggccttgg ccgaaatggc
    16141 agcgtatgtg tgcccgtctg ccgtggctgc tcgccattcc ccatgtggct tttgccacca
    16201 gcgcccttct gctctttgct gacggagggc tgctttatga ctatttcccg tatttcactc
    16261 cgctaatgga caaattgggt attgggctgg gcctcaccct tgcagtgaaa gaaagcgcat
    16321 ttctgctgtg gatcttagcg gcagtattga gcgaaaaacg gctgttgcag caggtcattg
    16381 tgctggattc gctgggctac agccgctggc aatgcctgaa ctggctgctc ttgccctccg
    16441 tcgcacctgc actggcaatg gcgatgttgg cgattgttgc ctggtcgctt tcggtcgtgg
    16501 atgtggcgat tattcttggg ccaggtaatc caccgacgct ggcagtaatt agctggcagt
    16561 ggttaactca gggcgacgcg gatcaacaaa cgaaaggcgc actcgccagt ctgctgctga
    16621 tgctgttact cgccgcctac gttttgctga gctatctgct atggcgcagc tggcggcgca
    16681 ctattccccg cgtagatggc attcgcaagc ctgccacacc tttattacca ggcactacgc
    16741 tggcgagttt tttaccctta accggtgtgc tgtgtgtggt tctgctggcg atcctcgcgg
    16801 atcagtcgac gatcaatagt gaagcactca tcaacagcct gacaatgggg ctggtggcga
    16861 cattcatcgc tttgctcctg ttactgctgt ggctggaatg ggggccacag cgtcgccagt
    16921 tgtggctctg gttgcccact ctattgcccg ctctgccact ggtggcgggc cagtacacgc
    16981 tggcgctatg gctgaacctg gatggaagct ggacggcggt ggtctggggg catctgctgt
    17041 gggtaatgcc gtggatgctg tttatcctgc aacccgcctg gcagcgcatt gattcacggt
    17101 taattttgat tgcgcaaaca ctgagctggt cgcgggccaa aatcttcttt tacgtgaaat
    17161 gtccgcttat gctacgccct acgctgattg ccttcgcggt gggattttca gtcagtattg
    17221 cgcagtatat gccgacgctg tggctgggcg cggggcgttt tccgacgctc accactgagg
    17281 cggtggcatt aagcagcggc ggcagcaatg gtattttcgc cgcccaggct ttatggcaac
    17341 tgctattacc gcttattatt tttgccctga ccgcgttagt cgcaaaatgg gtaggttatg
    17401 tcagacaagg actccgctaa tgctctgcgt gaaaaatgtt tcgctacgtt taccagaaag
    17461 ccgcttgctg acaaacgtta actttacggt ggataaaggt gacattgtca cgttaatggg
    17521 gccgtctggc tgtggaaaat ccactctgtt ttcatggatg attggtgcac tggctggaca
    17581 gttttcttgt acaggtgagc tatggctcaa tgagcaacgg attgacatgc tacccaccgc
    17641 acagcgtcag attggcattc ttttccagga tgcactgtta tttgaccagt tcagcgtcgg
    17701 gcaaaattta ctgctggcgc taccgtcgac gctcaagggg actgccaggc gcaatgccgt
    17761 gaaagacgcg cttgatcgag cgggactggc tgaaacttat caccaggacc ctgcgacatt
    17821 atcaggcggt cagcgagcgc gggtggccct gctgcgcgcc cttctcgctc agccaaaagc
    17881 gttactcctg gatgagccat tcagccgtct tgatgtggct ctgcgcgata attttcgcca
    17941 gtgggtgttc agcgaagttc gcgaactggc gatccccgtc gttcaggtaa cgcacgatct
    18001 ccaggatgtt cctgctgata gttctgttct ggatatggag cagtggtcag aaaattacaa
    18061 caaactgcga taacgcaaag tttttctcaa tgcgtcagtt cagaatggcg cactcaaaac
    18121 tacaatgtcg ggattttcga tgaaacgtgt ttctcaaatg accgcgctgg caatggcttt
    18181 agggctggct tgcgcttctt cgtgggccgc tgaactggcg aagcctctta cacttgacca
    18241 gcttcaacaa caaaatggca aagcgataga tactcgcccc agcgcgtttt ataacggctg
    18301 gccacaaacc ttaaatggcc cttctggtca tgaacctgcc gccttaaacc tctctgccag
    18361 ctggctcgac aaaatgagca ccgaacagct caacgcgtgg atcaagcaac ataacctgaa
    18421 agccgatgcc ccggtggcgc tgtacggtaa tgacaaagat gtcgacgccg tcaaaacgcg
    18481 actgcaaaaa gcaggtttta cgcatatctc cattctgagt gacgcgctaa gcgaaccttc
    18541 ccgtctacaa aaactgccgc actttgagca gctggtttat ccgcaatggc tgcacgacct
    18601 gcaacaaggt aaagaggtta cggcgaaacc tgccggtgac tggaaagtca ttgaagcggc
    18661 ctggggcgct cctaagcttt accttatcag ccatattccc ggtgctgact acatcgatac
    18721 caacgaagtg gaaagcgaac cgctgtggaa caaagtttct gatgaacaac taaaagcgat
    18781 gctggcaaaa cacggcattc gccatgacac cacggtcatt ctgtacgggc gtgacgtata
    18841 cgctggagcg cgtgtggcgc aaattatgct ctacgcaggc gtgaaagatg tgcgtctgct
    18901 ggatggcggc tggcaaacct ggtccgacgc gggactgcct gttgagcgag gaacgccacc
    18961 gaaagtgaaa gcggaaccgg atttcggcgt gaagatcccg gcacaaccgc agttgatgct
    19021 cgatatggaa caggcccgtg gactgctgca tcgccaggat gcatcgctgg tgagcattcg
    19081 ttcgtggcca gaatttatcg gtacgaccag cggttacagc tatattaaac caaaaggtga
    19141 aatagccgga gcacgttggg gacacgctgg tagcgactcg acgcatatgg aagattttca
    19201 taacccggat ggcaccatgc gcagcgccga tgatattacc gctatgtgga aagcgtggaa
    19261 tatcaaacca gagcagcaag tttcattcta ctgcggcacc ggctggcgcg cgtccgaaac
    19321 ctttatgtac gcacgcgcca tgggttggaa gaatgtttcc gtgtatgacg gcggctggta
    19381 cgaatggagc agcgatccaa aaaatccggt agcaaccggt gaacgcggcc cggacagtag
    19441 caaataacat tgcaattact gacgctggtg cgacttcagc gtcagatacc cactccacac
    19501 ccgcgtaaag gtcgtcatcc agcacaacgc accgaagatc cacgcaaacc acgcaaacca
    19561 tgcagggaat aaacatccca gcacaaacag taagatcgtt tctgtgcctt cggttaaccc
    19621 gcccagataa taaaacgatt tatgcgcata accgggatta tcaatctgat gtttcgccgc
    19681 cagcgcagca aaagcgagaa aactgctgcc tgtgccgata aacgcaaaca gcagccagcc
    19741 gcctgccagg gcgttttgct gcggtgcggc gaggataaac ccaaaaggta ccagcgcgta
    19801 aaaaagaaaa tcgagagaaa tatcaagaaa gccgcccgcg tcggtaagcc ctctgcgccg
    19861 cgccagcgca ccgtccagac catcaagcag cctgttcaac aaaatgacga caagcgccgc
    19921 cagataccag cccagcgcca gaaaaggcag tgccagcacg ccgatggcaa atcccactaa
    19981 tgttaaacca tctggcgtaa tgcccggttt atcaagaacc cgcacgcact gatgcaacaa
    20041 cggtttaatc cggggatgaa gatggcggtc tagcacaggc actccttaaa tataaagcct
    20101 ttctgattga gcaacagtgc ggatattatg gcatttttcg cttatctgcc cgtgtgtaat
    20161 ttatgaaaat gattgaagtt gttgccgcca tcattgaacg tgatggcaaa attttactcg
    20221 cgcaacgccc cgcccagagc gatcaggcgg gattatggga gtttgccggt ggtaaagtcg
    20281 agccggatga aagccagcgg caggcgctgg tgcgtgagtt acgcgaagaa ctgggcatcg
    20341 aagcaactgt gggtgagtat gttaccagcc atcagcgaga agtttcgggc cggattatcc
    20401 atcttcatgc ctggcacgta cccgacttcc acgggacgtt acaggcacat gaacatcagg
    20461 cgctggtctg gtgctcacct gaagaggcgc tgcaatatcc gctggcccct gctgacattc
    20521 cattattaga ggcgtttatg gctttacgcg ccgccagacc agcggattag tgctaagggt
    20581 tttgtcatcc cgctggcatt gcagcagtat tccctcggct ttaattaccg cgccttcaga
    20641 ataattttta tcctgataaa cgcagcactg agtacagggc tgcgctgact gcccgcctga
    20701 actgaacact tctggcggta cgtttacctc cacgtccgga cgataatgcg ggttagccag
    20761 tgcaattaat ggaaatgcta atactacggc gaacaatgct cgactcacag ggaactcctt
    20821 aacgttattg tctctgctac tgataacggt agccgggtgg caaaacttta gcgtctgagt
    20881 tatcgcattt ggttatgaga ttactctcgt tattaatttg ctttcctggg tcattttttt
    20941 cttgcttacc gtcacattct tgatggtata gtcgaaaact gcaaaagcac atcacataaa
    21001 caacataagc acaatcgtat taatatataa gggttttata tctatggatc agacatattc
    21061 tctggagtca ttcctcaacc atgtccaaaa gcgcgacccg aatcaaaccg agttcgcgca
    21121 agccgttcgt gaagtaatga ccacactctg gccttttctt gaacaaaatc caaaatatcg
    21181 ccagatgtca ttactggagc gtctggttga accggagcgc gtgatccagt ttcgcgtggt
    21241 atgggttgat gatcgcaatc aggtacaggt caaccgtgca tggcgtgtgc agttcagctc
    21301 tgccatcggc ccgtacaaag gcggtatgcg cttccatccg tcagttaacc tctccattct
    21361 taaattcctc ggcttcgaac aaaccttcaa aaatgccctg actaccctgc cgatgggcgg
    21421 tggtaaaggc ggcagcgatt tcgatccgaa aggaaaaagc gaaggtgaag tgatgcgttt
    21481 ttgccaggcg ctgatgactg aactgtatcg ccacctgggc gcggataccg acgttccggc
    21541 aggtgatatc ggggttggtg gtcgtgaagt cggctttatg gcggggatga tgaaaaagct
    21601 ctccaacaat accgcctgcg tcttcaccgg taagggcctt tcatttggcg gcagtcttat
    21661 tcgcccggaa gctaccggct acggtctggt ttatttcaca gaagcaatgc taaaacgcca
    21721 cggtatgggt tttgaaggga tgcgcgtttc cgtttctggc tccggcaacg tcgcccagta
    21781 cgctatcgaa aaagcgatgg aatttggtgc tcgtgtgatc actgcgtcag actccagcgg
    21841 cactgtagtt gatgaaagcg gattcacgaa agagaaactg gcacgtctta tcgaaatcaa
    21901 agccagccgc gatggtcgag tggcagatta tgccaaagaa tttggtctgg tctatctcga
    21961 acgccaacag ccgtggtctg taccggttga tatcgccctg ccttgcgcca cccagaacga
    22021 actggatgtt gacgccgcgc atcagcttat cgccaatggc gttaaagccg tcgccgaagg
    22081 ggcaaatatg ccgaccacca tcgaagcgac tgaactgttc cagcaggcag gcgtactgtt
    22141 tgcaccgggt aaagcggcta atgctggcgg tgtagcaacg tcaggccttg aaatggcgca
    22201 aaacgctgcg cgcctgggct ggaaagccga gaaagttgac gcacgtttgc atcacatcat
    22261 gctggatatc caccatgcct gtgttgagca tggtggtgaa ggtgagcaaa ccaactacgt
    22321 gcagggcgcg aacattgccg gttttgtgaa ggttgccgat gcgatgctgg cgcagggtgt
    22381 gatttaagtt gtaaatgcct gatggcgcta cgcttatcag gcctacaaat gggcacaatt
    22441 cattgcagtt acgctctaat gtaggccggg caagcgcagc gcccccggca aaatttcagg
    22501 cgtttatgag tatttaacgg atgatgctcc ccacggaaca tttcttatgg gccaacggca
    22561 tttcttactg tagtgctccc aaaactgctt gtcgtaacga taacacgctt caagttcagc
    22621 atccgttaac tttctgcgat agcagcagat atgccagtaa agaaacccca ttagactatt
    22681 tttttgataa tcttcttcgc tttcgaacaa ctcgtgcgcc tttcgagaag caagcattat
    22741 ataatgccag gccagttctt cttcaattgt cccgttttga aaagctgtgc ttgatatcga
    22801 ggtcatccat gataattccg ccgcccatat tagcttcgcc gaggatttac cggagcgatt
    22861 attagcgcaa tcagagatat agtctgaggg aaaaacagca aatttattca acaaggcgat
    22921 aacctgctct ggggcttcct ccatgtttgc tttaaaggta ttggctccat ggtcgccaga
    22981 aagaaaatac tccattaagg cacaataact ttcgctatct tcgatacccc attgatcctc
    23041 taaagactcg cgtcttttac ttatgatatc gatcgagtca aagggaagca catgatattg
    23101 gaaggtatct ttgccaggtt caggctttcg cggccagaac tccagcgttt cagaccattg
    23161 cttatgatag aaccgataag gtgcgatcaa ttgtagcgcc tgtaacttct cgatactgag
    23221 cggctcaata cctttagcct gataataatg cagttgttct ttttttgctt taaaaccggc
    23281 ccgaacaata agccccatca taattaatag ataaagaaaa gagcatcccg cggtaatcag
    23341 gcctctttca ttcaaaccgt tggatgttat cgctgcgaac acaaacatta cagcgacaac
    23401 acatgttaaa taaaaccccc acttacaaag cagcatggcc ttattttctt taatcatccg
    23461 ttcaaaatta ctattaaata tttcccagcc attaaaagaa tacttctcgc tcccaggatg
    23521 gttttgtaat aaaacttttt tcatcacatt ccctgttatt acatacttta gaggcgataa
    23581 ttttatccat gcaaaaaaaa tatccaacga attttcttga tccgctggat atttcagaat
    23641 atgactcgaa tagcacgaaa gattcactcg cttacgctat cgccccgctt ccgacttcat
    23701 ctgctggcgg actttttttc gcactacgtt tacgcggtgc agcctttttc ttatcagcac
    23761 tgccaccact gcccggagcc acaatgccgc gaaactgccg caccggcgta cgtttggctt
    23821 gatcaataag ctgatatagc gtccccacca gcggctgcat aaagtcctga tagcgacact
    23881 gcttttcgct gatttgcgtc agcaccgatt cccagtgcgc ggtcatgtcc ggtcgcgtcg
    23941 ccatttctgg cagcgaatgg aataacgctt ttccggcgtc ggtagagtgg atatagcgcc
    24001 cttttttggt cagaaaacca cgcttgaaca gcagttcaat aatcccggca cgggttgctt
    24061 ctgtccccag accatcggtc gcacgaagga tctttttcag atctttgtcc tgcacaaagc
    24121 gcgcgatccc ggtcatcgcc gaaagcagtg ttgcatcggt aaaatggcgc ggcggctggg
    24181 tttgccgctc taccacttca cctttttcac acagcaactc atcgcctttc gccaccacag
    24241 gcaatggcgt accgtcgttt tcttcatcgc gctctttgct gcctaacagc gtgcgccagc
    24301 ctgcttcagc aagaaaacgg gctttagcga caaatttgcc tttggcaatg tccagttcga
    24361 taacacactt gcggaacacc gcatccgggc agaactgcat caggtattga cgggcaatca
    24421 ggttatagac cttcgcttcg ttctccgtca ggttgatcgc agaactccgt gcggtcggga
    24481 tgatggcgtg gtgcgcatcg acctttttgt catcccaaca gcggttgcgt atatctggat
    24541 ctaccactga ctgcggcaac agatccggtg catgaacgct gatggcattc atcaccgcgt
    24601 ggcgtccggc aaaatgttct tctggcaaat agcgacaatc ggaacgcgga taagtgatta
    24661 gcttgtgcgt ttcgtacagc ttctggcaga tatcaagcac gttctgcgcg ctcagaccga
    24721 agcgttttgc cgcttcaatc tgtaacgccg aaagcgaaaa cggcagcggc gcggattctg
    24781 attcccgttt atcgttatag ctggtgacaa tagccggttg accactaatg cggttaacca
    24841 catgctccgc cagtggacga tgtaacaagc gcccttcttc atcctggtac ggttcacacg
    24901 cttcgctcgg ttgccagata gcggtaaacc gctcatcggc aggtgtcacg atatgcgctt
    24961 tgacttcaaa gaagtctttc gccacgaagt tttcaatctc ttcatcgcgg cgcaccacca
    25021 gcccgagcac gggtgtctgc acgcgtccca cggaaagtac gccctgataa ccggcattgc
    25081 gaccgagaat ggtatacgca cgggtcatat tgatgccgta cagccagtcg gcacgcgctc
    25141 gcgccagcgc agaaacgcac agcggtacaa actcactgtt ggaacgaaga cggtcgatcg
    25201 cccgctcaac cgcctgcggg ttcaggtcgt ttatcaagca acgctgcacc tgctggcgct
    25261 tttccggtgc cagttgcaga tagtccagca cttcatccac cagcaattgc ccttcacgat
    25321 ccgggtcccc ggcgtgaacg atttcgctgg cttcatgcag gaagcgtttg atgacgttaa
    25381 gttgtttggt cacggagggt cggggctgta attgccactt ttccgggaca atcggcaaat
    25441 ccgcaagatt ccagcgcgca tagcggctgt cgtaggcgtc tggctgcgcc tgctcaagca
    25501 ggtgaccgat acaccaggtc accacctgac cattaccgca ctcgataaag ccatcgcctt
    25561 tacggtgcgg tttgggcagg acatcagcaa tggcgcgcgc cagactcggt ttttcggcaa
    25621 taaacaaccg cattgaatta atggatctca accatggcac gaccgccgcg cgctggcacc
    25681 agttcgccaa tcgccgtcag ttcaatgcca aactcggcgg ctgtagtttt aacctcattt
    25741 tctgcttccg gcatgaccgc cagcagcaaa ccgccagaag tttgcggatc gcacagcaga
    25801 tcgcgcactt cacgcggcat ttcacccatc agatgaccgt agctggcaaa gttacgttcg
    25861 gtgccgccag gcactgcgcc caacttaatg tactcttcaa cccccggcaa ttttgggatc
    25921 gcgtcatagt caacgcgcgc ctgcacacct gcgccctgac acatttcgct caaatggccc
    25981 agcagaccaa aaccagtaac gtcggtcatc gcttttacgc cttcgatgtt ggcaaaggac
    26041 gcacctgcga tgttcatccg gcacatcact tccgtcgcca gtccctgatg ttctggtttc
    26101 aacagtgatt ttttctcagc cgtggtaaga acgccgatcc ccagcggttt cgtcaggaac
    26161 agtttgcatc cggcttgtgc ggtactgttt ttcttcaccc gctcggtcgg tacgatcccc
    26221 gttaccgcca gaccaaaaat cggctccggc gcatcgatgg aatgaccgcc agccagcgca
    26281 atacccgcct gacggcatgc atagcgtcca ccttcggtta cttcgcgggc aatttctggg
    26341 gaaagtttgt taatcggcca gccgaggatc gcaatcgcca taatcggttt gccccccatc
    26401 gcgaagatat cgctgatggc gttagtcgcc gcaatgcggc caaaatcgaa aggattatca
    26461 acgatcggca taaagaagtc ggtggtactg ataacgctgg tgccattgcc cagatcgtac
    26521 accgccgcat cgtcgcgggt ctcattaccc acaagcaaat tcggatcaac aaacttcgcc
    26581 tgctcactgt gcaggatggt ttccaacact tttggggaaa ttttacagcc gcaaccagct
    26641 ccgtggctgt attgggtcaa acgaatcgag ttctcgctca tggacatctc ctgtcaatgc
    26701 aatccgggta tggtaaccct cattccgtga agtgataagt gagagtgtct gaattcctgc
    26761 gcctttgctc acaatccaga cagtttcgcg acaattatca gaaataagtt acaaacggcg
    26821 tcgggtccgg gacgttaatc gacgtagatg ctttcaactg cggcgtgccg aggtagagaa
    26881 aaccgacaat tttatcctgc tcacggcaac cgaatgcttc acgcactacc ggactttcag
    26941 ttaatgcgcc actgcgccag atgccgccaa atccttgggc aacggctgcc atttgcatcg
    27001 ccatgaccgc gcatccggca gacatttcct gttcccatcg cgggacttta tgattttctt
    27061 cgcatttcgc caccaccgtg atgatgagcg gtgcgcggaa cggcgcatta cgggctttgt
    27121 cgatagcttt gtcatcacta ccggcagcaa tcgccccctg ttccagtacg gcgctgaaac
    27181 gctcgcgccc ttccccttca atcacaaaaa aatgccacgg ttgcatggac ttatggtccg
    27241 gcgcacgcat acccgcacgc aggatgtttt gcagttgttc acccgttggc gcgggttcag
    27301 ccaggcggga ggcgctacgg cgattgatca atagttcgag tgcatccatt tgattaactc
    27361 ctgtcgtgat atttattcac aaaattaaca cgagagtgga ttttgttaca gcacagtccg
    27421 caattcctgc tgacaagtgc cggttgggtc attacgataa ccacatctat tgcgcctgtg
    27481 acaggtgtga ccttaagttg ggagaataca tgcgaaccct ttggcgattt attgccggat
    27541 tttttaaatg gacgtggcgt ctgctgaatt tcgtccgtga aatggtactt aacctgttct
    27601 ttattttcct cgtactggtt ggtgtgggga tctggatgca ggtcagtggt ggtgattcga
    27661 aagaaacggc cagtcgtggc gcactgctgc tggacatttc tggtgtgatc gtcgataaac
    27721 ccgacagttc tcagcggttt agtaaattaa gccgccagct gcttggtgcc agttccgatc
    27781 gtctgcagga aaactcactg tttgatatcg tcaacaccat tcgccaggcg aaggacgacc
    27841 gcaatatcac cggtatcgtg atggatctga aaaacttcgc aggcggcgac cagccgtcta
    27901 tgcagtacat cggcaaagcc ctgaaagagt ttcgtgacag cgggaaaccg gtttatgccg
    27961 ttggcgagaa ctacagccag gggcaatatt atctcgccag tttcgccaat aaaatttggc
    28021 tgtcaccgca aggcgtggtt gatctgcacg gttttgctac caacggtctg tactacaaat
    28081 cgttgctgga taagctgaaa gtttccaccc atgtgttccg cgtgggtacg tataaatctg
    28141 ccgttgaacc gtttattcgt gatgatatgt caccggcagc ccgcgaagct gacagccgct
    28201 ggattggtga gctgtggcaa aactatctga atactgttgc cgctaaccgg cagatccctg
    28261 ctcagcaggt attccctggc gcgcaagggt tgcttgaggg tttaaccaaa accggtggcg
    28321 ataccgcgaa atatgcactg gaaaacaagc tggtcgatgc actggcatcg agtgccgaaa
    28381 tcgaaaaagc tctgaccaaa gagttcggct ggagtaagac tgataaaaat tatcgcgcca
    28441 tcagttatta cgattacgca ttgaaaacgc cggcagatac cggtgacagc atcggtgtcg
    28501 tcttcgctaa tggcgcaatt atggatggcg aggaaactca ggggaatgtt ggcggtgata
    28561 ccactgcggc acaaatccgc gacgctcgcc ttgacccgaa agtgaaagcg attgtcctgc
    28621 gtgttaatag cccaggcggc agcgttaccg cgtctgaagt gattcgcgct gaactggcag
    28681 cagcccgggc agcgggtaag cctgtggttg tatcgatggg cggcatggcg gcatctggtg
    28741 gttactggat ttccacgcca gctaattaca ttgtggctaa ccccagcacc ctgaccggtt
    28801 ctatcggtat cttcggcgtg atcaccaccg tagaaaatag tctggattcg attggtgttc
    28861 atactgatgg tgtctcaact tcaccgctgg cggatgtttc tatcaccagg gcactgccgc
    28921 cggaagcgca gcagatgatg caattaagca ttgagaatgg ctataaacgc tttatcacgc
    28981 tggttgctga tgcgcgtcat tcgacgccgg agcaaattga taaaatcgcc cagggccacg
    29041 tctggaccgg tcaggatgca aaagctaacg ggctggtcga tagtctcggg gatttcgatg
    29101 atgcggttgc caaagcagca gagctggcaa aagtgaaaca gtggcatctg gaatactacg
    29161 ttgatgaacc gaccttcttc gacaaagtga tggacaacat gtctggttct gtccgggcaa
    29221 tgttgccaga tgcgttccag gccatgttac ctgcaccgct tgcctcggta gcctctaccg
    29281 ttaaaagtga aagcgacaag ctggccgcgt ttaacgaccc acaaaaccgt tatgcgtttt
    29341 gcctgacctg cgccaacgtg cgttaagtct tgtactgagt ggccgacaga tcgtcggcca
    29401 cattattttt tacctcgacg aatcctcttc ccgctgtctc gacccatatt tccttatccg
    29461 catagtatca ggtgcgctcc tcctgcctca cgtatatact tttgctcttt cgatatcatt
    29521 catatcaata tcatgcaaaa gaaatcaatt tacgtcgcct acacgggcgg gaccatcggg
    29581 atgcagcgtt ccgagcaggg ttatattccg gtgtcaggtc atctacaacg ccaactggcg
    29641 ctgatgccgg aattccatcg cccggagatg ccagatttca ccattcatga atatacgccg
    29701 ttgatggatt cttctgatat gacgccggaa gactggcagc atattgctga agatattaaa
    29761 gcgcactatg acgactatga tggttttgtc attctacacg gtaccgacac gatggcgtat
    29821 accgcctctg cgctgtcgtt catgctcgag aatctcggta aaccggtcat tgtgacaggg
    29881 tcacaaatcc cgctggctga gttacgctct gacggacaaa ttaatctgct gaatgcgttg
    29941 tacgttgcgg cgaattatcc gatcaacgaa gtaacgctct ttttcaataa ccgattgtat
    30001 cgcggcaacc gcactaccaa agcccatgcc gatggttttg atgcgtttgc ctctccaaac
    30061 cttcctccgt tactggaagc tggtatccat attcgtcgtt tgaatacgcc acccgccccg
    30121 cacggtgaag gggaattaat cgttcatcca atcacgccac aaccaattgg cgtagtgacg
    30181 atttatccag ggatttctgc tgacgtcgtg cgcaattttc tgcgccaacc ggtgaaagca
    30241 ttgattctgc gctcctatgg cgtgggtaat gcgccacaaa acaaagcctt cctgcaggaa
    30301 ttacaagaag ccagcgatcg cggtattgtg gtggtcaacc tgacacaatg tatgtccggt
    30361 aaagtgaata tgggtggtta tgccaccggt aacgccctcg cccatgccgg cgttattggc
    30421 ggtgcagata tgactgtaga agccacgcta accaaactgc attacctgct gagccaggaa
    30481 ctggatactg aaaccattcg caaggccatg agccaaaacc tgcgcggcga actgacgccg
    30541 gatgattaag gagactgcaa taccccatcg cgccctgtta ctggtcgatt tacaaaatga
    30601 tttctgtgct ggtggcgcgc tcgccgtgcc ggaaggtgac agtacggtag atgtcgctaa
    30661 ccgcctgatt gactggtgcc agtcgcgcgg tgaagcggtt atcgccagtc aggactggca
    30721 cccggcgaat cacggcagtt ttgccagtca gcacggtgta gagccttata cgccaggcca
    30781 actcgacggt ttgccacaaa ccttctggcc agatcactgt gtgcagaaca gtgaaggcgc
    30841 acaattacat ccgttactga accaaaaagc gatcgcagcg gtgttccata aaggcgaaaa
    30901 tcctttagtt gacagttaca gtgccttttt tgataacggc cgtcggcaga aaacctctct
    30961 cgatgactgg ttacgcgatc atgaaatcga tgaattgatc gttatgggcc tggctactga
    31021 ctattgcgtg aagtttaccg tgctggacgc gttacagtta ggttataagg taaacgtgat
    31081 taccgatggt tgtcgtggcg tgaatatcca gccccaggac agtgcgcacg cgtttatgga
    31141 gatgtcagca gctggggcaa cgctatatac gctggcagac tgggaagaga cgcaggggta
    31201 atattagttg aattcatctc tggcctacat ttcctcaatg acattgtagg ccagataata
    31261 cttgtcagtg ttgcatttaa cctacttgca gctaaaacac gacgtcctaa caagtatatt
    31321 tgccctccct taataacagg cttaaatact agataactca ttcacttaaa catagcaata
    31381 ggctttgctg agataccaaa atcttctttt aactgctgtt tgctcttcat gaccatctga
    31441 ccatttgtgt caattgtcat atgctgtgct tcggtattat ggcgggcttg ccacagcatc
    31501 accagttgca ggcaattctc tttttgttcc tcggtcaacg caacgccatc aggccatttc
    31561 cccagttcaa cggcggtcga caaacgctgg tatacctcag gcgtcatgct gttgataatg
    31621 tcatcaagat tcataatttt tccgctcctg tggaataagt tgctgaatcg ttttttcaac
    31681 cgttgatttc ttcgccgttt tcgccatcag tgaagcgtaa agaagcagag ttaacacaat
    31741 aacgttcgcc tgttggttgc ggcccgtcgg gaaagacatg ccccagatgg gcatcacagt
    31801 taccgcaacg aatttctatg cgctgcattc catgtgacaa gtctttgata taacgaatgg
    31861 attcttcact caccggttcg tagaaactgg gccagccaca gccggaatca tacttggttt
    31921 cggaatgaaa cagcggggca tcgcagatca aacagtgata tacgccgtca cgcttgttat
    31981 gcagtaaacg acccgtaaat ggcggttctg tcccatgatt ctgcgtcacg taaaactgca
    32041 tctcggacaa attttttttc agttcttctg ccgaaggttt attagccatt tgctcacatc
    32101 tcactttaat cgtgctcaca ttacgtgact gattctaaca aaacattaac accaactggc
    32161 aaaattttgt cctaaacttg atctcgacga aatggctgca cctaaatcgt gatgaaaatc
    32221 acatttttat cgtaattgcc ctttaaaatt cggggcgccg accccatgtg gtctcaagcc
    32281 caaaggaaga gtgaggcgag tcagtcgcgt aatgcttagg cacaggattg atttgtcgca
    32341 atgattgaca cgattccgct tgacgctgcg taaggttttt gtaattttac aggcaacctt
    32401 ttattcacta acaaatagct ggtggaatat atgactatca aagtaggtat caacggtttt
    32461 ggccgtatcg gtcgcattgt tttccgtgct gctcagaaac gttctgacat cgagatcgtt
    32521 gcaatcaacg acctgttaga cgctgattac atggcataca tgctgaaata tgactccact
    32581 cacggccgtt tcgacggtac cgttgaagtg aaagacggtc atctgatcgt taacggtaaa
    32641 aaaatccgtg ttaccgctga acgtgatccg gctaacctga aatgggacga agttggtgtt
    32701 gacgttgtcg ctgaagcaac tggtctgttc ctgactgacg aaactgctcg taaacacatc
    32761 accgctggtg cgaagaaagt ggttatgact ggtccgtcta aagacaacac tccgatgttc
    32821 gttaaaggcg ctaacttcga caaatatgct ggccaggaca tcgtttccaa cgcttcctgc
    32881 accaccaact gcctggctcc gctggctaaa gttatcaacg ataacttcgg catcatcgaa
    32941 ggtctgatga ccaccgttca cgctactacc gctactcaga aaaccgttga tggcccgtct
    33001 cacaaagact ggcgcggcgg ccgcggcgct tcccagaaca tcatcccgtc ctctaccggt
    33061 gctgctaaag ctgtaggtaa agtactgcca gaactgaatg gcaaactgac tggtatggcg
    33121 ttccgcgttc cgaccccgaa cgtatccgta gttgacctga ccgttcgtct ggaaaaagct
    33181 gcaacttacg agcagatcaa agctgccgtt aaagctgctg ctgaaggcga aatgaaaggc
    33241 gttctgggct acaccgaaga tgacgtagta tctaccgatt tcaacggcga agtttgcact
    33301 tccgtgttcg atgctaaagc tggtatcgct ctgaacgaca acttcgtgaa actggtatct
    33361 tggtacgaca acgaaaccgg ttactccaac aaagttctgg acctgatcgc tcacatctcc
    33421 aaataagttg agatgacact gtgatctaaa aagagcgact tcggtcgctc ttttttttac
    33481 ctgataaaat gaagttaaag gactgcgtca tgattaagaa aatttttgcc cttccggtca
    33541 tcgaacaaat ctcccctgtc ctctcccgtc gtaaactgga tgaactggac ctcattgtgg
    33601 tcgatcatcc ccaggtaaaa gcctcttttg cattacaggg cgcacacctt ctctcgtgga
    33661 aacctgcggg tgaagaagaa gttctgtggt tgagcaacaa cacaccgttc aaaaatggcg
    33721 tcgctattcg cggtggcgta ccggtttgct ggccgtggtt tggtccggcg gcacaacaag
    33781 gtctgcctgc gcacggtttt gcccgcaacc tgccgtggac gctgaaatca catcgtgaag
    33841 atgctgatgg cgtagcgctg acttttgaat tgacgcaaag cgaagagacg aaaaaattct
    33901 ggccgcacga ctttacgctg ttagcgcatt tccgcgtggg taaaacctgt gaaatcgatc
    33961 ttgaatcaca tggcgaattt gaaaccacct ctgccctgca tacctacttt aacgtgggtg
    34021 atatcgctaa ggtaagcgtc agtgggctgg gcgatcgctt cattgataaa gtaaatgacg
    34081 cgaaagaaga tgtactgacc gacggtattc agaccttccc tgaccgtacc gatcgcgtgt
    34141 atctgaatcc gcaagattgc agcgtgatta atgatgaagc gctgaatcgt attatcgccg
    34201 taggccacca gcatcatctg aacgttgtcg gctggaaccc gggaccggcg ctttcagtta
    34261 gcatgggcga catgccggat gatggctaca aaacatttgt ttgtgtagaa acggcttacg
    34321 cttcagaaac gcaaaaagtg accaaagaga aacctgcaca tctggcgcaa tccattcgcg
    34381 ttgcgaaacg ttaatttgcg ttaatgttgt gtgccgggtg cgatgcatcc ggcacgtatc
    34441 aacatcacac catatccagc gcagtttttc cttttggtgc cggatacgct ttatccagca
    34501 tggttaattc cgctgacgaa agttcgacct caagcacagc cgcattctgt tggacatggg
    34561 caatcgtggc cgcttttgga atcgccatca caccctgatg gctgattacc cacgccaaca
    34621 atacttgtgc cgcgctgata ttgtgagcat gtgcaatttc gttgactacc gcgtttttta
    34681 acagtccatt gcgcaaccgc ccggcctggg ctaatggact gtaagccatc accggcatct
    34741 gctgttgctg gcaccagggg agtagatcgt actcaatccc tcgtgaaccg agatggtaaa
    34801 gcacctgatt agtggcacac tgatttcccc ccggcagctg ccagagttcc tgcatatcag
    34861 catgatcaag gttagaaacg ccccagcggc ggatttttcc ctgggcgatc aatttttcca
    34921 tcgctgccac agtctcttca aaagcgaaac tgccagacca gtgtaataag tagagatcga
    34981 gataatcagt attgagacgg cgtaaactgg cttcgcatgc atttatcgct ttttgcccgc
    35041 cagcattcca cggatagact ttagagacga gaaagacgtt atcgcgcaga ccggttaatg
    35101 cttccccaac caccttttcg gcaccgccat cggcatacat ttcggcggta tcaatgaggg
    35161 ttaaaccgag ctcaatgccc gcgcgtagtg cagcaacttc tgttttgcgc tgactggcat
    35221 cttcgcccat ataccatgtt ccctgcccta tggctggcag tgagacatcg ccactaaatt
    35281 gaatcatttt ttgttgcatt gtttcctccc aggtattgca ccaccgtaat gcaaaacagg
    35341 gcgtgacgcc ctgtttttat gcacaaaatg ccctggaaag atgcattatc agaatttgta
    35401 ggtgatcccg gtagaaatca ggccagtcca ggatttatcc accatcgggc tgtcagtaac
    35461 ttcatcagac agacgggtat agcgcgcggt accgtaaaca ctccagtcgc cgaggaagtt
    35521 gtagctggcg ctcagctcca ggtaagggct ccagctgtcg ttcgggttat agccacgcag
    35581 accgctgcga gcggactctt tgcgcgatac gccataatag tattcgttct ggttttcgct
    35641 gttccactgc acaccgatac ccggggtcac ggtcaggccg ccgttggtgt aacgatacaa
    35701 ccaggccata tcccagacga tgccgttgct gttatccagg gtatcgccag ccagggtggt
    35761 acgcaggtaa ccgtactggg taaagtgagc ataagacaga ccagccatca tggtgctctt
    35821 acggtcatcc aggtgacgca tttggtgatc gccactgtct ttcgctttga agtaaagcgg
    35881 cgaccaataa gcggtaattg aaagtttgtc cgttgcgtca ttccacaggt agtaaccacc
    35941 acctaagcca cggaaccaga agttatcgcc ttcatagttg attaccggta ctgggtaaac
    36001 atcggtatcg taatctttat atgggtgctc aacgacacct acgcctgcgc ccagggaaaa
    36061 tttaccttca gcgtgcgcta cgcctgcaga cgttgcgata agcactccaa gtgccagaag
    36121 tttgagtttg gtcacaatta atcattcctt aaacaaatgt ttagcgggcg acaaagttta
    36181 cccgtcaata catcgaaacc caaccttttt acgttttcat tttttaaagt aactgtttaa
    36241 ttttcctgac gcggatgaca ccgcgcttac agccaaatga atttagcgtt actggctacc
    36301 ctggtcttta cattaattat gcaaaattta tggatgagtt gttgatatgc cattgaaatt
    36361 aagaaagccg tgcaggcaag ttttccattt gccatctacg cttaattttg aaggtgtatc
    36421 accgggcacg ttgttctcat cgtcgataaa atggcatgag agttgctgtg ttttagcaag
    36481 agacgtcgtt cagtttacct cttccgggag cctctactat tcatatgaac ggctcttaac
    36541 ctgtgctaaa aaacgaaagg acggcatacc atgaatatat tcgatcacta tcgccagcga
    36601 tatgaagctg ccaaggacga agagttcaca ctgcaggagt ttcttaccac ttgtcggcaa
    36661 gatcgcagtg cttatgccaa cgcggctgag cggctattga tggctatcgg tgagcctgtc
    36721 atggtcgata cagcccagga acccagactt tctcgactct tttctaaccg ggtcattgca
    36781 cgttatccgg catttgaaga gttttacggc atggaagacg cgattgaaca gattgtctct
    36841 tatctgaaac acgcggctca ggggctggaa gagaagaaac aaatcctgta tctgctgggg
    36901 cctgtgggtg ggggtaaatc atcgcttgct gagcgactga aatcattaat gcagctcgta
    36961 ccgatttatg tattgagcgc caacggtgag cgtagcccgg tcaacgatca tccgttctgt
    37021 cttttcaatc cgcaggaaga tgctcagatt ctggaaaaag agtatggcat tcctcgccgt
    37081 tatctcggca ccatcatgtc gccatgggcg gcaaaacgcc tgcatgaatt tggtggcgat
    37141 atcactaagt tccgggtagt gaaggtctgg ccgtcaattc tgcaacaaat tgctatcgcc
    37201 aaaacggaac ccggtgatga gaacaaccag gacatctccg cgctggttgg gaaagtcgat
    37261 attcgtaaac tcgaacacta cgcgcagaat gacccggacg cctacggcta ttccggtgcg
    37321 ctgtgccgcg ccaaccaagg gatcatggaa tttgtcgaga tgtttaaagc accgataaaa
    37381 gtgctgcatc cgctgttaac cgccactcag gaaggtaact acaacgggac ggaaggtatc
    37441 tccgccctgc cgttcaacgg gattattctc gcccactcga acgaatccga atgggtcact
    37501 ttccgtaata acaaaaacaa cgaagccttc ctcgaccgtg tttacatcgt gaaggtgccg
    37561 tattgcttac gcatttccga agagatcaaa atctacgaga aattgcttaa tcacagtgaa
    37621 ttgactcacg ccccctgcgc ccctggcacg ctggaaacgc tgtcacgttt ttccattctt
    37681 tcgcgcctta aagagccaga aaactccagc atttattcaa agatgcgggt ttatgatggc
    37741 gaaagtctga aagacaccga tcccaaagcc aagtcgtatc aggaatatcg tgactacgcc
    37801 ggtgtcgatg aagggatgaa cggtctgtcg acgcgttttg cgtttaagat cctctcccgc
    37861 gtgttcaact tcgatcatgt agaagtggcg gcaaacccgg tccatctgtt ctacgtcctg
    37921 gaacagcaga tcgagcgcga gcagttccca caagagcagg cagaacgcta tctggagttc
    37981 ctgaaaggtt atctgatccc gaaatatgcc gagtttatcg gcaaagagat ccagacggcc
    38041 taccttgaat cctattccga atatgggcaa aacattttcg accgttatgt tacctacgcg
    38101 gatttctgga ttcaggatca ggagtatcgc gatccggata ccgggcagct gtttgaccgc
    38161 gagtctctta acgccgagct ggagaaaatc gagaaaccgg cggggatcag taatccaaaa
    38221 gatttccgca acgagattgt taacttcgta ctgcgcgcca gagcgaataa cagcggacgc
    38281 aatccgaact ggaccagcta tgaaaaactg cgcacggtta tcgagaagaa aatgttctcc
    38341 aataccgagg agctgttgcc ggttatctcg tttaacgcca aaacgtcaac cgacgagcag
    38401 aagaaacacg acgactttgt cgaccgtatg atggaaaaag gctacacccg taaacaggtg
    38461 cgtttactgt gcgaatggta tttgcgcgta cgtaaatcgt cttaacaacc ctggcccggt
    38521 acacatgtta ccgggcctac aacgacagcg aaccgtgggc ctgagaagcg gcaacacagg
    38581 cgtagcatac agttggcaaa tgtagtacgg ggggcatatg acctggttta ttgaccggcg
    38641 tctgaacggc aaaaacaaaa gcatggtgaa tcgccagcgt tttttacgcc gttataaagc
    38701 gcaaattaaa cagtcgatct ccgaggccat taataagcgt tcggtgactg acgtcgacag
    38761 cggcgaatcc gtatccattc ccacggaaga tattagcgaa ccgatgtttc atcaggggcg
    38821 tggcggtctg cgccaccgcg tgcatccggg caatgaccat ttcgtccaga acgaccgaat
    38881 tgaacgtccc cagggtggcg gcggaggttc cggtagtggt cagggccagg ccagccagga
    38941 tggtgaaggt caggatgaat ttgtctttca gatttcgaaa gatgagtatc ttgatttgct
    39001 ctttgaagat ttggccttac cgaatctgaa acaaaaccaa caacgccagc tgaccgaata
    39061 taaaacgcat cgggcgggtt ataccgctaa cggcgttccg gcaaatatca gcgttgtgcg
    39121 ctccttgcag aactcactgg cgcgacgcac agccatgacg gcaggcaagc ggcgggaact
    39181 tcatgcactg gaagagaatt tggccatcat cagcaacagt gaacctgcgc agctgctgga
    39241 agaggaacgt ctgcgcaaag aaattgcaga attacgtgcc aaaattgaac gcgtcccttt
    39301 tattgacacc ttcgatttac gttacaagaa ctacgagaag cggcccgatc cctccagcca
    39361 ggcagtgatg ttttgcctga tggacgtttc cggttcaatg gatcaatcca ctaaagatat
    39421 ggctaagcgt ttttatattc tgctgtatct gttcctcagc agaacgtata agaacgtgga
    39481 agtcgtatac atccgccatc atacccaggc gaaagaagtc gatgaacatg agtttttcta
    39541 ctcgcaggaa acaggcggca ccattgtttc cagcgccctg aaactgatgg atgaggtagt
    39601 gaaagagcgt tataacccgg cacagtggaa tatttacgct gcacaagcat cggacggcga
    39661 taactgggcc gatgactctc cgctttgcca tgaaatcctg gcgaaaaaat tattacctgt
    39721 tgttcgttat tacagctata tcgaaattac ccgtcgtgca catcagacat tgtggcgaga
    39781 atatgagcat ctgcaatcta ctttcgacaa ctttgcgatg cagcacatcc gcgaccagga
    39841 tgatatttat ccggtgttcc gtgaactgtt tcataaacaa aatgcaacag ctaaagacta
    39901 aaactatcag ccaggtcatt atcgcctggc tgatttttag cttact
//
LOCUS       DAZGJW010000027        39833 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZGJW010000027 DAZGJW010000000
VERSION     DAZGJW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.027"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..85)
                     /locus_tag="ACHKIK_001471"
     CDS             complement(<1..85)
                     /locus_tag="ACHKIK_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFO9082671.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMA"
     gene            complement(85..1293)
                     /gene="ybjJ"
                     /locus_tag="ACHKIK_001472"
     CDS             complement(85..1293)
                     /gene="ybjJ"
                     /locus_tag="ACHKIK_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9082672.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            1377..1913
                     /gene="rcdA"
                     /locus_tag="ACHKIK_001473"
     CDS             1377..1913
                     /gene="rcdA"
                     /locus_tag="ACHKIK_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO9082673.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(2088..3773)
                     /gene="ybjL"
                     /locus_tag="ACHKIK_001474"
     CDS             complement(2088..3773)
                     /gene="ybjL"
                     /locus_tag="ACHKIK_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO9082674.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            4043..4420
                     /gene="ybjM"
                     /locus_tag="ACHKIK_001475"
     CDS             4043..4420
                     /gene="ybjM"
                     /locus_tag="ACHKIK_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO9082675.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(4450..4707)
                     /gene="grxA"
                     /locus_tag="ACHKIK_001476"
     CDS             complement(4450..4707)
                     /gene="grxA"
                     /locus_tag="ACHKIK_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO9082676.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            4867..5154
                     /gene="ybjC"
                     /locus_tag="ACHKIK_001477"
     CDS             4867..5154
                     /gene="ybjC"
                     /locus_tag="ACHKIK_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO9082677.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            5138..5860
                     /gene="nfsA"
                     /locus_tag="ACHKIK_001478"
     CDS             5138..5860
                     /gene="nfsA"
                     /locus_tag="ACHKIK_001478"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO9082678.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            5921..6823
                     /gene="rimK"
                     /locus_tag="ACHKIK_001479"
     CDS             5921..6823
                     /gene="rimK"
                     /locus_tag="ACHKIK_001479"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO9082679.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            6911..7387
                     /gene="ybjN"
                     /locus_tag="ACHKIK_001480"
     CDS             6911..7387
                     /gene="ybjN"
                     /locus_tag="ACHKIK_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO9082680.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            7738..8850
                     /gene="potF"
                     /locus_tag="ACHKIK_001481"
     CDS             7738..8850
                     /gene="potF"
                     /locus_tag="ACHKIK_001481"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO9082681.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHVYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDNVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            8945..10078
                     /gene="potG"
                     /locus_tag="ACHKIK_001482"
     CDS             8945..10078
                     /gene="potG"
                     /locus_tag="ACHKIK_001482"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO9082682.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            10088..11041
                     /gene="potH"
                     /locus_tag="ACHKIK_001483"
     CDS             10088..11041
                     /gene="potH"
                     /locus_tag="ACHKIK_001483"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO9082683.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            11038..11883
                     /gene="potI"
                     /locus_tag="ACHKIK_001484"
     CDS             11038..11883
                     /gene="potI"
                     /locus_tag="ACHKIK_001484"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO9082684.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            11943..12431
                     /gene="ybjO"
                     /locus_tag="ACHKIK_001485"
     CDS             11943..12431
                     /gene="ybjO"
                     /locus_tag="ACHKIK_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO9082685.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            12472..13599
                     /gene="rlmC"
                     /locus_tag="ACHKIK_001486"
     CDS             12472..13599
                     /gene="rlmC"
                     /locus_tag="ACHKIK_001486"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO9082686.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(13774..14505)
                     /gene="artJ"
                     /locus_tag="ACHKIK_001487"
     CDS             complement(13774..14505)
                     /gene="artJ"
                     /locus_tag="ACHKIK_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO9082687.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(14797..15465)
                     /gene="artM"
                     /locus_tag="ACHKIK_001488"
     CDS             complement(14797..15465)
                     /gene="artM"
                     /locus_tag="ACHKIK_001488"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO9082688.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(15465..16181)
                     /gene="artQ"
                     /locus_tag="ACHKIK_001489"
     CDS             complement(15465..16181)
                     /gene="artQ"
                     /locus_tag="ACHKIK_001489"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO9082689.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(16188..16919)
                     /gene="artI"
                     /locus_tag="ACHKIK_001490"
     CDS             complement(16188..16919)
                     /gene="artI"
                     /locus_tag="ACHKIK_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO9082690.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(16937..17665)
                     /gene="artP"
                     /locus_tag="ACHKIK_001491"
     CDS             complement(16937..17665)
                     /gene="artP"
                     /locus_tag="ACHKIK_001491"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO9082691.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(17883..18398)
                     /gene="ybjP"
                     /locus_tag="ACHKIK_001492"
     CDS             complement(17883..18398)
                     /gene="ybjP"
                     /locus_tag="ACHKIK_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO9082692.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            18524..18847
                     /gene="ybjQ"
                     /locus_tag="ACHKIK_001493"
     CDS             18524..18847
                     /gene="ybjQ"
                     /locus_tag="ACHKIK_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO9082693.1"
                     /translation="MQFSTTPTLEGQIIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            18844..19674
                     /gene="amiD"
                     /locus_tag="ACHKIK_001494"
     CDS             18844..19674
                     /gene="amiD"
                     /locus_tag="ACHKIK_001494"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO9082694.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(19671..20684)
                     /gene="ybjS"
                     /locus_tag="ACHKIK_001495"
     CDS             complement(19671..20684)
                     /gene="ybjS"
                     /locus_tag="ACHKIK_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO9082695.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWTV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(20783..22213)
                     /gene="ybjT"
                     /locus_tag="ACHKIK_001496"
     CDS             complement(20783..22213)
                     /gene="ybjT"
                     /locus_tag="ACHKIK_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO9082696.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYSTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(22224..23225)
                     /gene="ltaE"
                     /locus_tag="ACHKIK_001497"
     CDS             complement(22224..23225)
                     /gene="ltaE"
                     /locus_tag="ACHKIK_001497"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO9082697.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(23262..24980)
                     /gene="poxB"
                     /locus_tag="ACHKIK_001498"
     CDS             complement(23262..24980)
                     /gene="poxB"
                     /locus_tag="ACHKIK_001498"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO9082698.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(25113..26081)
                     /gene="hcr"
                     /locus_tag="ACHKIK_001499"
     CDS             complement(25113..26081)
                     /gene="hcr"
                     /locus_tag="ACHKIK_001499"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO9082699.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(26093..27745)
                     /gene="hcp"
                     /locus_tag="ACHKIK_001500"
                     /gene_synonym="priS"
     CDS             complement(26093..27745)
                     /gene="hcp"
                     /locus_tag="ACHKIK_001500"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO9082700.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(27889..28788)
                     /gene="lysO"
                     /locus_tag="ACHKIK_001501"
     CDS             complement(27889..28788)
                     /gene="lysO"
                     /locus_tag="ACHKIK_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO9082701.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTSLAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(29283..29978)
                     /gene="aqpZ"
                     /locus_tag="ACHKIK_001502"
     CDS             complement(29283..29978)
                     /gene="aqpZ"
                     /locus_tag="ACHKIK_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO9082702.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            30404..32062
                     /gene="ybjD"
                     /locus_tag="ACHKIK_001503"
     CDS             30404..32062
                     /gene="ybjD"
                     /locus_tag="ACHKIK_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO9082703.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HSSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(32059..33015)
                     /gene="ybjX"
                     /locus_tag="ACHKIK_001504"
     CDS             complement(32059..33015)
                     /gene="ybjX"
                     /locus_tag="ACHKIK_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO9082704.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            33166..34281
                     /gene="macA"
                     /locus_tag="ACHKIK_001505"
     CDS             33166..34281
                     /gene="macA"
                     /locus_tag="ACHKIK_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO9082705.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            34278..36224
                     /gene="macB"
                     /locus_tag="ACHKIK_001506"
     CDS             34278..36224
                     /gene="macB"
                     /locus_tag="ACHKIK_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO9082706.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(36297..36521)
                     /gene="cspD"
                     /locus_tag="ACHKIK_001507"
     CDS             complement(36297..36521)
                     /gene="cspD"
                     /locus_tag="ACHKIK_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO9082707.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            36671..36745
                     /gene="yljB"
                     /locus_tag="ACHKIK_001508"
     CDS             36671..36745
                     /gene="yljB"
                     /locus_tag="ACHKIK_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO9082708.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            36844..37164
                     /gene="clpS"
                     /locus_tag="ACHKIK_001509"
     CDS             36844..37164
                     /gene="clpS"
                     /locus_tag="ACHKIK_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO9082709.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            37195..39471
                     /gene="clpA"
                     /locus_tag="ACHKIK_001510"
     CDS             37195..39471
                     /gene="clpA"
                     /locus_tag="ACHKIK_001510"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO9082710.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
BASE COUNT         9720 a        10026 c        10618 g         9469 t
ORIGIN      
        1 gagccataaa ccgctcacgg ttataggttt tttgatcgct taagaaagta ccatccatgt
       61 ctaccgcaat taatttaatg ctcatcaact attctccatc gccgtctgcg ttttggtatc
      121 gggtttggcg acggctttcg cgacaatagc cgcgagaata accagcgcca gtacaaccag
      181 cattgcacta cgtaatccat aatgttcgcc gagatagccc agcagcggcg gcccgacgag
      241 gaaagccaga taaccggtcg ttgctaccac actgacgcgg gttggtgcat cggggccggt
      301 atcgctggcg gcagaaatgg tcagcgggaa gcccagcgag gcacccagtc cccagagtac
      361 aacagacacc ccagcgaccc aggcgctatc gacaaaaata atcagcccaa tacccaacgc
      421 tcccattagt gcactggccc gaaccacggc aacgcgactg taacggtcga tgaaccaacc
      481 gccggtaaag cgtccaacag tcatccccag ggtaaaaccg gcataaatca gcgagccgga
      541 agtagggcta aaaccgtgac cgtcaaccat taataagggt aaccagtcgt tggcagaacc
      601 ttcggcaaag gccatcgcca gcaccacaac accaatcagc agcaactgga tatcgcgata
      661 aaaaggtacg cctttttcac catgctgggt gccatcggca gcatttttgc ccgtaccgtc
      721 agggattgcc tgaatggcga tataaatagg cgcgattcct accagcgccg ccaataaaat
      781 gtgcaccgtt gccggaacgc caaaggccgt cagtgccatc ccgacaccag cgcctgccag
      841 cgtgcccagg ctataaaaac cgtgcatcat cggcaaaacc gttttattca tttctcgctc
      901 aacggcggca ccttcgacgt ttatcgccac ttccgcagaa ccaaaacttg ccccaaagac
      961 gccgaggcca accgcgaaga gcaggggtga tgtcagccag agtgccagac ttaatatcat
     1021 catcccgatc aatgcgcagg acatcgtgac caggatgaca ttacgtgtcc caaagcgttt
     1081 cactaaccac gccgagcaga gaataccgct catcgaaccg atcgacagac caaagagaac
     1141 accgcccatt tcagcgatcg agacagagag aatatcgcgg atagcaggcg tacgggttgc
     1201 ccaggacgcc attaacaggc ccggcaaaaa gaagaacata aacagcgccc aggttcggcg
     1261 tttcaatgca ttacgtgaag aatttacggt catagatcac gtcaaaataa gaagagggaa
     1321 gacaacatta gcaaggttgt gtacatttgt acacaattgc agacagagga aatgacatgc
     1381 gtcgcgctaa cgatccgcaa cggcgagaaa aaattatcca ggccacgctg gaggcggtga
     1441 aactttacgg aatacatgct gttacgcacc gcaaaattgc tacccttgcc ggggtaccgt
     1501 tgggatcgat gacctactat ttttcaggaa ttgatgagtt gttgctggag gcgttcagca
     1561 gttttactga gattatgtcc cggcaatatc aggcattttt tagcgatgtt agtgatgctc
     1621 aaggcgcatg ccaggctatc accgatatga tctacagctc acaggttgca acgccggata
     1681 acatggagct gatgtaccag ctctacgcgc tggctagccg aaaaccgcta ttaaaaacgg
     1741 taatgcaaaa ctggatgcag cgcagtcagc aaacgctcga acaatggttt gaacccggaa
     1801 ccgcccgcgc gcttgatgcg tttattgagg ggatgacgct gcattttgtc accgaccgta
     1861 agccgctatc gcgcgaggag attttgagga tggttgagag ggttgcaggg tagtagataa
     1921 attttagata acaaaaaacc catcaacctt gaaccgaaat ggcggggttg atgggctcca
     1981 caaaatgggg acatcaaaga aaagcagtgg cactaattaa gactgatgcc ctgcggaaaa
     2041 gttctgcggt tgtgcaaaaa aatttcattt tcagggcaac ttcagtttta tcctaatcct
     2101 ggccatacca tgacgatgat tgtccctgcc agcgtcagca ggacgttggc gattgcgtag
     2161 gtgcccgcat agcccagcgc agggatgtta ctgcgagctg tatcactgat gatctccatt
     2221 gccggcgcgc aggtgcgtgc gcccatcatt gcgccgaaca acagtgcgcg gttcattcgc
     2281 aatacataag caccgaacaa gaaacagata accacgggca caagactgac aattaatccg
     2341 gcaatcaaca tctgaccgcc aatcgcgccc aggccgttat taataccgct accggcgctc
     2401 agaccaacgc ctgccataaa caccatcaag ccgaactctt tcaccatgct taatgcacct
     2461 tgcggaatgt aaccgaaggt cgggtggtta gcacgcataa agcccagcat aattccggcg
     2521 aataacaacc cggcagcgtt ccccatgccg aaactgaatg tgctgaactg gaaggtgatc
     2581 atcccgatca tcagcccaat aacaaagaag gcgcaaaatg ccagcaggtc agtgacctgg
     2641 ctgtgaatcg agataaagcc gatgcgatcg gcgatggttt ttacgcgacg ggcatcaccg
     2701 ctgacttgta aaacgtcacc tttgttaagc acgacgttgt catctatcgg catctcaatc
     2761 tggctacgaa tgacgcggtt aaggaagcaa ccgtgatcgg tcaacttcag ttgtgcgaga
     2821 cgtttaccta cagcgttatg gtttttaacg accacttctt cagtgacgat acgcatgtcg
     2881 agaaggtcac gatcgaaaac ttctttaccg ttacggaagc tgggatcgag tcgggcatgg
     2941 gcgtcgggat agcctaccaa cgctatttca tcgcccattt gtagcacggc atcaccgtct
     3001 ggatttgcca gaatcccgtt acgtcgaata cgttcaatgt agcagccggt ttgtcgataa
     3061 atacccagtt cacgcagatt tttgccgtcg gtccaggcca ccagctccgg gccgacgcga
     3121 taggcgcgga tcaccggtaa ataaacctta cggttggcat cagtgtccag gccacgttcg
     3181 cgggcgattt gctgggcgct ggtctgtaag tcctgatgct gcaatttcgg caagtaacgc
     3241 gcaccaacaa tcaaactcac cagaccgatt aaataggtta aggcataccc gaggctcaga
     3301 ttatccagtg ccagtgagag ctgcctgctt tccatgccgg aatgacgcag tgtatcgcca
     3361 gcaccgacca gaaccggtgt cgacgtcata gagcctgcta acataccggc cgtcaggcca
     3421 atatcccagc caaacagctt acctaaccct aaggcgatca ccagcgcact gccaaccatc
     3481 accagtgcta acattaggta atttttccca tcgcgaaaaa aaatggaaaa aaagttcggt
     3541 ccggcttcga ccccgacgca gaaaataaac agcataaagc caagattaag cgcatcggtg
     3601 ttaatgctga aatgttgttg gcctaataac agcgatacga ctaaaacgcc aatggaatta
     3661 cccagttgga tcgaaccaag tcgtaacttt ccgagacata gcccaagcgc gaggaccaca
     3721 aataataaca gaatgtaatt cccatttaac aattcggcga cgtttatatt cacggaggct
     3781 aacttcttgt ttactagtaa gctgttgaaa gaaatggtaa tttacgataa tgttttttac
     3841 cagaattcag ggcgcagatt cattcagcgc acctaaacga tagtaaagta acaatatatt
     3901 ttactagtgt aatcacatta ggtatcaacg gctatatgaa ttgcgttggc ctatattagc
     3961 atggaatgcg aagcggcttt atcttactga acgccacact ggcgaaaaat gtgttcgata
     4021 gacgcagtgt caggaggaac gagtgaaaca taaacaacgt tgggcggggg caatctgctg
     4081 ttttgtcctc ttcattgtgg tgtgcctttt tctggcgacg cacatgaaag gcgcttttcg
     4141 ggctgccggg catcctgaaa tcggcttgct atttttcatt cttcctggag cagtcgccag
     4201 cttcttttca cagcgtagag aagtcctgaa acctctattt ggcgcaatgc tggcggcacc
     4261 ctgttcaatg ctcattatgc ggctgttttt ttcaccgacg cgctcattct ggcaagagct
     4321 ggcatggtta ctaagcgcgg tgttctggtg tgcgctgggg gcactgtgtt tcttatttat
     4381 cagtagtttg tttaaaccac agcacagaaa aaatcagtaa agccctcaac gcgagggctt
     4441 gtcagacgat caggcgtcca gattttcttt cacccatgca gcaaaatcgg tatagccgcc
     4501 gatatgttgc tgatcgacaa aaatctgcgg cacggtttct acgggtttac ctgccttttg
     4561 ttgtagatct tctttagtga tcccttccgc acgaatatct acatactgat actgaaaatc
     4621 atcgcgttca ttgctcaatt tctcagccag atcttttgca cgcacacagt aagggcaacc
     4681 cgaacgacca aaaataacgg tttgcattat ttctctcctc atagatttat gcctgtaatg
     4741 atcacgctaa aatgtattcg ctgaaagtag gtttaacctg ttgcattaat tgctaaaagc
     4801 tataactgtt aaacacaata cagtgaaaag ttttagactg aaggctcact ttgcagaggg
     4861 aagcgtatgc gcgcgatcgg taaattgcct aaaggcgtgt tgatactgga atttatcgga
     4921 atgatgctac tggcggtggc gctgctgtcg gtaagcgact ccctgtcgct gcctgagcca
     4981 ttttctcggc cagaagtgca gattctgatg atttttctcg gtgttttgct catgcttccc
     5041 gctgcggtgg tggttattct tcaggtggca aaacgtcttg ccccacagct gatgaaccgt
     5101 ccaccgcaat attcacgttc agaaagagaa aaagataatg acgccaacca ttgaacttat
     5161 ttgtggccat cgctccattc gccatttcac tgatgaaccc atttccgaag cgcagcgtga
     5221 ggcgattatt aacagcgccc gtgcgacgtc cagttccagt tttttgcagt gcagtagcat
     5281 tattcgcatt accgacaaag cgttacgtga agaactggtg acgctgaccg gcgggcaaaa
     5341 acacgtagcg caagcggcgg agttctgggt gttctgtgcc gactttaacc gccatttaca
     5401 gatctgtccg gatgctcagc tcggcctggc ggaacaactg ttgctcggtg tcgttgatac
     5461 ggcaatgatg gcgcagaatg cattaatcgc agcggaatcg ctgggattgg gcggggtata
     5521 tatcggcggc ctgcgcaata atattgaagc ggtgacgaaa ctgcttaaat taccgcagca
     5581 tgttctgccg ctgtttgggc tgtgccttgg ctggcctgcg gataatccgg atcttaagcc
     5641 gcgtttaccg gcctccattt tggtgcatga aaacagctat caaccgctgg ataaaggcgc
     5701 actggcgcag tatgacgagc aactggcgga atattacctc acccgtggca gcaataatcg
     5761 ccgggatacc tggagcgatc atatccgccg aacaatcatt aaagaaagcc gcccatttat
     5821 tctggattat ttgcacaaac agggttgggc gacgcgctaa aaccgccacg tcgatgtatg
     5881 atacgcgggc ttttgaccag gtctgacaga gaggtgcagg gtgaaaattg ccatattgtc
     5941 ccgggatgga acgctctatt cgtgtaagcg gctgcgtgaa gccgctatac agcgcggtca
     6001 cctggttgaa attcttgatc cgctttcttg ctacatgaac ataaatcctg cggcgtcttc
     6061 tattcactac aaaggccgca agttacccca ttttgacgca gtgatcccgc gtattggcac
     6121 cgccattacc ttttatggga cggcggcact gcgccagttc gagatgctgg ggagctatcc
     6181 gctcaatgag tcggtcgcca ttgcccgggc gcgtgacaaa ttgcgttcca tgcaactgct
     6241 ggcgcgtcag ggcatcgacc tgcctgtcac gggcattgcg cattcgccgg atgataccag
     6301 cgatttaatc gacatggtcg gtggtgcgcc gctggtggtc aagttggttg aaggcacgca
     6361 gggaattggc gtcgtgctgg cggagacgcg tcaggcggcg gaaagcgtga ttgacgcttt
     6421 ccgcggtctg aacgcgcata ttctggtgca ggaatatatc aaagaggcgc aagggtgcga
     6481 tatccgctgt ctggttgttg gcgatgaagt ggtcgctgcg attgaacggc gggcgaaaga
     6541 gggcgatttt cgttccaatt tgcatcgtgg cggcgcggca agcgtcgcca gtatcacacc
     6601 acaggagcgt gaaatcgcga taaaagccgc gcgaacgatg gcgctggacg ttgctggtgt
     6661 ggatattctg cgtgctaatc gcgggccgtt ggtgatggag gtgaatgcgt cgccggggct
     6721 ggaaggaata gaaaaaacca ccggtatcga catcgcgggt aaaatgatcc gctggatcga
     6781 acgccacgct acgacagaat attgcctgaa aacgggtggt tagtcgcaat cacattactg
     6841 atcatggttt tgcctgcgct ttttgcgtaa gctgtgccgg tctttttatc gaaagaggtt
     6901 gtacaaaatt atgacatcgc tggtcgttcc tggtctggat acgctgcgtc aatggctcga
     6961 tgacctgggg atgagttttt ttgaatgtga taactgtcag gctctgcatc tgccccatat
     7021 gcagaatttc gacggtgtct ttgatgccaa aatcgatctg atcgataaca cgatcctgtt
     7081 ttctgccatg gcggaagtcc gaccttcagc cgtattgccg ctggcggcgg atttatctgc
     7141 catcaatgcc agttcgctga ccgtgaaagc atttcttgat atgcaggatg ataatctgcc
     7201 aaagctggtg gtttgccagt ctttatccgt tatgcagggc gtaacctatg agcagtttgc
     7261 atggttcgtg cgtcagagcg aagagcagat ttcgatggtc attcttgaag ctaatgccca
     7321 tcaactgctg ttaccgactg acgatgaagg gcaaaacaac gttaccgaaa actatttcct
     7381 ccactgataa ctcctttcga gcacgcagtc gctggtgcag tggctgcgcg ctgcaaaatt
     7441 atctgctgtt tttaaccttt tcttaaagat tatttcactt ctcttgtgtc gatttggctt
     7501 tatcacatag agcaaatacg cataaaaatt tgttaaatac cgttttttaa tccgagctat
     7561 agtctcaaac cctggctaaa gttattcttg cgatgctttt atatagcgag cagtgctggc
     7621 cgggagagag ttctcttttc ttacaccccg ccgataaaaa atatgcacgt ttattgcata
     7681 tcttttagtg tgacaacttt tgttcgtttg ttaacgaact ttcagaagga aagagatatg
     7741 accgccttaa ataaaaaatg gctttcgggt ctggctgcgg gtgctctgat ggccgtctct
     7801 gtcggcacgc tcgcggctga acaaaaaaca ctccacgttt ataactggtc tgattatatc
     7861 gccccggaca cggtggccaa ttttgaaaaa gaaaccggta ttaaagtcgt ctacgatgtt
     7921 ttcgactcta acgaagtact ggaaggcaaa ttaatggcgg gtagcaccgg ctttgacttg
     7981 gtggttccat ctgccagctt tcttgaacgc cagttgactg cgggagtttt ccagccgctg
     8041 gacaaaagca aattgccgga gtggaagaat ctcgacccgg agctgctgaa gctggtcgcc
     8101 aaacacgatc ccgacaataa atttgctatg ccctatatgt gggcgacgac cgggattggc
     8161 tataacgttg ataaagttaa agctgtgctg ggcgaaaacg cgcccgtcga tagctgggac
     8221 ttgatcctca aacctgaaaa tctggaaaaa ctgaaaagct gcggtgtctc tttcctggat
     8281 gcgccagaag aagtttttgc taccgtgttg aattatctcg gcaaagatcc caacagcact
     8341 aaagcggatg attacaccgg accggcaacc gatctgctgt taaagctgcg cccgaacatt
     8401 cgttatttcc attcatctca atacattaac gacctggcaa acggcgatat ttgcgtcgct
     8461 atcggctggg caggtgatgt ctggcaggcg tcaaaccgcg cgaaggaagc gaagaatgga
     8521 gtgaatgtct cgttctcgat tccaaaagaa ggggcgatgg cgttctttga tgtattcgcc
     8581 atgcctgcgg atgccaaaaa caaagacgaa gcctatcagt tcctgaatta cctgctgcgc
     8641 ccggatgtag tagcgcatat ttccgacaat gtgttctatg ccaacgccaa taaagcagcc
     8701 acgccgctgg tgagtgcgga agtccgtgag aacccgggta tttatccgcc tgcggatgtt
     8761 cgtgcgaagc tgttcactct gaaagtgcag gacccgaaaa tcgaccgtgt gcgcacccgc
     8821 gcgtggacca aagtgaagag cggaaaataa tccgcagcct tagatgccgg gcgggcgcac
     8881 cacacccgcc ggcaattcgc accatcatgg tgcgcttgca cacattcaat gccggagagc
     8941 agccgtgaat gacgctatcc ctcgcccgca ggcgaaaacc cgtaaggcgc tgacgccgct
     9001 attagaaatc cgcaacctca ccaaatccta cgatggtcaa catgcggtgg atgatgtcag
     9061 cctgaccatc tacaaaggtg aaatcttcgc gctgctgggc gcatccggct gtggcaagtc
     9121 cacgctgctg cgtatgctgg caggtttcga acaaccttct gccggacaga taatgcttga
     9181 tggcgtcgat ttgtcacagg tgccgcctta cctgcgcccc atcaatatga tgtttcagtc
     9241 ttacgcgctg tttccccata tgactgtgga gcagaacatc gcctttggcc tgaaacagga
     9301 caaactaccg aaagcggaaa ttgccacccg ggtcaacgag atgcttgggc tggtgcacat
     9361 gcaggagttc gccaaacgca aaccgcatca gctttccggt ggtcagcgac agcgtgtggc
     9421 cctggcccga agccttgcca aacgcccgaa actattactg ctcgatgagc cgatgggcgc
     9481 gctggataaa aagctgcgtg accggatgca gcttgaagtg gtggatattc tggagcgcgt
     9541 cggtgtgact tgtgtgatgg tcacccacga tcaggaagag gcgatgacca tggcggggcg
     9601 tatcgccatt atgaatcgcg ggaaatttgt ccagattggc gagccggaag agatctacga
     9661 gcatccaact acccgctaca gcgctgaatt tatcggttcg gttaatgtct ttgaaggtgt
     9721 actcaaagag cgtcaggaag atggcctggt gcttgattcg ccggggctgg tgcatccact
     9781 gaaagtcgat gcggatgctt cagtggtcga taacgtgccg gtacacgtag cgctgcgccc
     9841 ggaaaaaatc atgctttgcg aagagccgcc cgccaatggt tgtaacttcg cggtggggga
     9901 ggtgatacac attgcctatc tcggcgatct ttcggtgtat cacgttcgtc tgaaaagtgg
     9961 gcagatgatt agcgcccagc tacaaaacgc ccatcgtcat cgtaaagggt taccgacctg
    10021 gggcgacgaa gtgcgtttgt gctgggaagt ggacagctgt gtggtgctga cggtttaagg
    10081 agcaaagatg aatacacttg aacctgctgc ccagtcgaaa ccgccgggcg gatttaagct
    10141 gtggttgtcg cagctgcaaa tgaagcatgg gcgcaaactg gtcattgcgt tgccatatat
    10201 ctggttgatc ttgctgtttc tgctgccatt tctgattgtc tttaaaataa gcctggcgga
    10261 gatggcgcgc gctattccac cttataccga actgatggag tgggctgacg ggcaactgtc
    10321 catcactctt aatctcggta attttctgca actgaccgac gatccgctct atttcgatgc
    10381 ttatctccag tcgctacagg tggctgcgat ttcgacattt tgctgtttac tgatcggcta
    10441 tccgctggcg tgggcggtgg cgcacagtaa gccttcgacc cgtaatattt tattactact
    10501 ggtgatcctg ccgtcgtgga cctcgtttct gatccgcgtt tatgcctgga tgggaatatt
    10561 aaaaaacaac ggtgtgctga ataattttct gctgtggctg ggggttatcg atcaaccgct
    10621 gaccattctt cataccaatc tggcagttta tattggcatt gtttacgcct acgtgccgtt
    10681 tatggtactg ccgatttata ccgcgttgat tcgtattgat tattcgctgg tggaagcagc
    10741 gctggatctc ggtgcacgac cgctgaaaac gttctttact gtgatcgtgc cgctgactaa
    10801 aggtgggatt attgccggat cgatgctggt gtttatcccg gctgtgggcg agtttgtgat
    10861 cccggaactg ctcggtggcc cggacagcat catgatcggg cgcgtgctat ggcaagagtt
    10921 tttcaataac cgcgactggc cggtggcctc ggcggtagcg atcatcatgt tgctgctgct
    10981 aattgtgccg ataatgtggt tccacaaaca ccagcaaaaa agcgtgggag aacacggatg
    11041 aataatttac cggtagttcg ttcgccctgg cggattgtga ttttgctgct gggctttacc
    11101 tttctctacg cgccaatgct gatgctggtt atctattcgt ttaacagctc gaagctggtg
    11161 acggtgtggg ccggctggtc aacgcgctgg tatggtgagt tattgcgcga tgacgcgatg
    11221 atgagtgcgg ttggtttaag cctgacaatt gctgcctgtg cggcaacggc ggcggcgatc
    11281 ctcgggacta ttgcggcggt ggtgctggtg cgctttggca ggtttcgcgg atcaaatggc
    11341 tttgccttta tgatcaccgc gccgctggtg atgccagatg tcatcacggg cttgtcgctg
    11401 ttgttattat tcgtcgcgct tgctcatgcc attggctggc ctgcggaccg cggtatgctc
    11461 accatctggc tggcgcatgt cactttttgt acggcttatg tggcggtcgt tatttcttca
    11521 agattgcggg aactggatcg ctcgatagaa gaagcagcga tggatctcgg tgcgacgccg
    11581 ctgaaagtgt ttttcgtcat tacgctaccg atgatcatgc ccgcgatcat ttctggctgg
    11641 ttactggctt ttactttgtc gcttgatgat ctggtgatcg ccagctttgt ttccgggccg
    11701 ggagccacca cgttaccgat gctggtcttt tccagcgtgc ggatgggggt gaatccggaa
    11761 atcaacgccc ttgcaacgtt aattctcggt gcggtcggaa ttgtcggatt tatcgcctgg
    11821 tatctgatgg ctcgcgcaga aaaacagcgg atacgcgata tccagcgtgc aagacatggc
    11881 tgaagacact aaaatttgcc aacctggcta cataatgccg cgcatgccgc ggcattgttt
    11941 tcatggaaga cgaaacgttg ggatttttta agaaaacatc ttcatctcat gctcgcctga
    12001 atgtgcctgc gctggtgcag gtggcggcgc tcgccattat tatgatccgt ggcctcgacg
    12061 tgctgatgat tttcaatacg ctgggcgtgc gcggtattgg cgagttcatt catcgcagcg
    12121 tacaaacctg gagtttaacg ctggtctttt taagcagtct ggtgctggtt ttcattgaga
    12181 tctggtgtgc gttttcactg gtgaaagggc gtcgctgggc gcgctggcta tatctgctga
    12241 cacaaatcac cgccgcaagt tacttgtggg cggcttcgct ggggtacggt tatccggagc
    12301 tgttcagcat tcccggtgaa tcaaaacgtg aaatcttcca tagcctgatg ctgcagaagc
    12361 tgccggatat gctcatcctg atgctgctgt tcgttccctc gaccagtcgg cggttcttcc
    12421 agttgcaata atgtgtataa tcgtcgcccc tgatgatgtg aaggtcaatg tatgcagtgc
    12481 gcactttacg acgcgggtcg ctgtcgttcc tgtcagtgga taacgcagcc gattccagag
    12541 caactctccg ctaaaaccgc cgatcttaaa aatctgctcg ccgattttcc ggttgaggaa
    12601 tggtgcgcgc cggtgtcagg cccggaacaa gggtttcgta ataaagccaa aatggtggtg
    12661 agtggtagcg ttgaaaaacc actgctcggt atgctgcatc gcgacggcac accagaagac
    12721 ctttgtgact gcccgcttta tcctgcctca tttgcgcccg tttttgcggc gctaaaaccg
    12781 tttatcgccc gtgcggggct gacaccctac aacgtggcgc gtaagcgtgg cgaactgaaa
    12841 tacattctgc tgactgaaag ccagagcgat ggcggcatga tgctgcgctt tgtactgcgt
    12901 tctgaaacca aactggcaca actgcgtaag gcgctgccgt ggttacagga acaactaccg
    12961 caactgaaag ttattaccgt caatattcag ccggtacata tggcgattat ggaaggggag
    13021 acggagatct accttactga acaacaggcg ctggcagagc gttttaatga cgtaccgctg
    13081 tggatccgtc cgcaaagttt cttccagact aatccggcgg tcgccagcca gctgtacgct
    13141 accgcgcgcg actgggtacg gcagctgcca gttaaacata tgtgggatct gttctgcggt
    13201 gtgggtggct ttggtttaca ctgcgcgacg cctgacatgc agttaaccgg gatcgaaatt
    13261 gcatcagagg ccattgcctg tgcaaagcag tcagccgctg aactgggctt aacgcgtttg
    13321 caatttcagg cgctggactc cactcagttt gccaccgctc agggggaggt gccggagctg
    13381 gtgctggtta atccgccgcg ccgcggcatt ggtaaaccgc tatgtgatta tctctcaaca
    13441 atggcaccgc gttttattat ctactccagc tgtaacgccc aaaccatggc gaaagatatc
    13501 cgcgaactgc caggttaccg catcgaacgg gtacagcttt ttgatatgtt cccgcacacc
    13561 gcgcactatg aagtgctgac gctgctggtg aagcaataaa aaagccgcag gtgcggcttc
    13621 agattgctga tcaggtgcgc tttgttcatg ccggatgcgg cgtgtacgcc ttatccggcc
    13681 tacaaaatca tgctaaatca atacattgca gagttgatgt gggccggata agcgcagcgc
    13741 atcaggcaat cttacttttg tcatcagtct caattactgc ggaaaccact ggtcattgat
    13801 tttctgatat gtaccgtcag ctttaattgc tgccagcgcg ttattcagtt tttccagcag
    13861 agctttgtta tccggacgta cagcgatgcc cagaccggtg ccgaagtatt gcggatcggt
    13921 cactttttca gtagcaacgc ccagttgcgg attggttttc agccattcgt ttaccaccgc
    13981 tgtgtcacca aataccccat caatacgacc atttttcaga tcgataaagg cattctgata
    14041 actgtcatag gagacagttt tcacttcggg gtgctgatcc tgaatatatt tctggtgcgt
    14101 ggtgccgttt tccatcccaa tacgtttgcc tttcagatcg gcaaacgttt tgtaggtatc
    14161 ttttttggca atcacgacgg ctgagttttc atagtagggc gtggtaaacg atacctgctt
    14221 gctacgctcg ggcgtgatat ccataccgga gattacggcg tcatattttc tgaatttcag
    14281 tgacgggatc aggctgtcga acgcgtgatt agtaaaagta cattctgcct gcatttgttt
    14341 gcacaaggct tttgccagat cgatatcaaa gccgacaatc tcattattag cacctataga
    14401 ttcaaagggt ggataggtgg ctgaaacgcc aaaattgatt ttctctgcgg cagaagcacc
    14461 gaaagtaaag gaagcaagta aagcggcaag aactaacttt ttcatgatgg aactcccgtc
    14521 tgtcaatctt atgatttttg gccgtgtctg cggcatggga taacaatgcc atcaagtgaa
    14581 tttatatgca ataaatatga ttaaataatt taaatgaaat aaaaaaagac ggatacctta
    14641 gtgagttgtc cgtcttcatt ataagaattt atgcactatg taggccggat aaggcgtcca
    14701 cgccgcatcc ggcacaggca ccgtgctgat gtctgatgcg acgctggcgc gtcttatcag
    14761 acctacaaac ccccccggcg aatgtacgca gctacattaa tttcgccgtt cgaatgccag
    14821 tgctttgcgc tcgatcagac gcatcatcag cgtcagcagg ccgttgacga ccaggtaaat
    14881 aatccctgcc gcaccgaaca ccattacatc gtaggtgcgt ccgtacaaca actggctgta
    14941 tcccatcact tccatcagcg taatggtgta agccagagag gtacttttga acaccagcac
    15001 cacttcgttg gaataagaag agagcgagcg tttaaaggca tacggcagca ggatcgccag
    15061 cgtatctttt ttgctcattc ccagggcgct acaggactgc cactgacctt ctgggatcgc
    15121 acgaattgca ccgtaaaaca gctgcgtggt atacgccgca ctgttcagcg ataacgcaat
    15181 cagcgcacat aaccacggtt ctgacaacaa atgccacagt gccggatact cctgcaaagt
    15241 cggaaactgg cccggcccgt aataaatcag gaagatctgc accagcagcg gcgtaccggt
    15301 aaacagcgtg atataacccc gcaccagcca caccagcacc ggcgttttca gcgtcaggat
    15361 gatggtaaaa atcaatgcca gaatcagtgc cacaatcagc gaggcaacgg ttagcgtcag
    15421 gctggtgtgt agccctttca tcagttcggg taaatactca aacattagct gggcctccgc
    15481 tcaaaacgtg tcgcgcgcag gtcaatgcgt ttgagaatgt actgactgag cagggtgatc
    15541 accaggtaaa tcgccgccgc cacaatgtac caggtaaatg gttcctgggt acgagtagcg
    15601 atgctttttg tttgcagcat taaatcattc acactaatca aactgaccag cgcggtatct
    15661 ttcagcagca ccagccactg gttaccgagg ccaggcagcg catgacgcca catctgcggc
    15721 atcaccagac ggaaaaagat agccgatttc gacagcccca gcgcctgacc agactcccac
    15781 tgacccaccg gcaccgcttt caacgcgccc cgaagcgttt gcgaggcata ggcggcatac
    15841 agcagtgaca gagcgatgac accacaaagg aacgggctaa cgtcgaagtt ctcaatgtcc
    15901 atctgcactg ggatctgcac gaacccaaga ttgatagtga agccatccga aagcgtcagc
    15961 agcagctgcg aggagccaaa atagataaac agcaccacca gaatttctgg caggccacgc
    16021 agaatggtta ccagcgctga acctgcccac gcgacaggac gccattttgc cgactcccat
    16081 accgcaaaga acatcgccag cgccagcccg acaatcaatg cacaaacggc aaggccgacg
    16141 gtcatcccgg cggcgcttgc taaaggaaaa aattcattca tcaggaatta cttctggaac
    16201 catttgttgt agatggtttc gtaagtgcca tctttcttca ctttttccag cgcagtgttg
    16261 agtttctgct gcagctcagt gttgccctga cgtaccgcga tgccgaggcc agtgccgaag
    16321 taatctttat cggtcacttt gtcgcccacc gccgccagtt tcgggttatc tttcagccac
    16381 tcagtgacca ctgcggtgtc accgaagacg ccgtcgatac gcccgttttg cagatccagt
    16441 tttgcgttct ggtagctgtc atacggaacg gtagtgattt ccgggtgctt atccataatg
    16501 aatttctggt gtgtcgtccc gttctgtacg ccgacttttt tgcctttcag ctgatcaaca
    16561 ctggtgtatt tgccttgctg acccacaaac agggcagagt tgtcatagta cggggtggta
    16621 aacagcacct gcttttcacg ctccggagtg atatccatgc ccgccatcac ggcttctacg
    16681 cgacggaatt tcaggcttgg gatcaggctg tcaaacgcct ggttagagaa agtgcaggtt
    16741 gcatcaatct ctttacacag cgcttgtgcc aggtcgacgt caaaaccaac gatctggttg
    16801 tttgcatcaa tcgattcaaa cggaggatag gatgcttcgg tagcaaaacg aatggtttcg
    16861 gcagctgtgg cggaaagact aaaacctgca attaacgcgg caatcagaac ttttttcatt
    16921 gttgttatcc cgaatcttag tgagagagat agtttttaaa tgcttcggtt tgcggctcgg
    16981 taaagcagct cgcgtcgcct tgttctacga tatgaccatt ttccatatac accactcggc
    17041 tggcggtttt acgcgccact tcaacttcgt gggtgacgat cacctgggta atattcgttt
    17101 ctgccagctc acgaatgatg ctgacgattt gtgccgtaat ttccgggtcc agtgcggcgg
    17161 tcggttcatc gaacagcagt acctgcggtt ccatcatcaa cgcacgggca atagcaacac
    17221 gctgctgctg accaccagaa agatgcagcg ggtaacgatc gctataaggt ttgagacgca
    17281 gacgttccag cagtttttct gcacgggcca gcgcctgatc tttactcaac cccagtacac
    17341 ggcagggcgc ttcaatcagg ttttgctgca cggtcagatg cggccacagg ttgtattgct
    17401 gaaacaccat gccaacgtta cgacgcaaat cgcgaatcgc tttgtcagag ggtgttttgg
    17461 tgaaatcgaa atggttgcct gcaatgttga gcgtaccgga gcgcggcatc tcaagcagat
    17521 tgagtacacg cagcagcgag cttttacccg cgccgctggg gccaagtaac accagcgttt
    17581 cgccctgtgg gcaatccagc gtgatatcga acagcgcctg atgcgcgccg tagaagcaat
    17641 taatgccgtt taattgaata ctcattgaca ctcgtatact ggcagtctga tagctattga
    17701 ggtcgaagat agtacctttg acagaataat tatgcaatat ttctgcttta aaagttaaaa
    17761 gcaaagcgca ttattcaata aacatagcac aaaataacgg gggcggtggt cggcgagcat
    17821 aaatgtcggc attcctcacg aaatgccgga caatttacgg ggtttattgg ttgatcaagg
    17881 cgttagcgat tctcgatgga ctgacggagc gtacccgccg tggcatgaac gctgccgcct
    17941 aaatagcgta catcgtcgat cacccaacac tggccttcct gaatcattaa cacttcatcc
    18001 tgccagctct gatcaccctg ttttagatcc acacgcagag gaatatttcg cgcatcacga
    18061 ttcgggattg ttgaggcact ggcaacacgg gcgctctctg gcaaggttgt gcgactggag
    18121 aatggatcgt tggtcagtag ttcccgatgg ttattatccc gggaggcatc gctaagcagt
    18181 gtcgccagtt tgtcgctcag ataagggcgc aaggcggtga tgtcgttgct gcggtgcaaa
    18241 atgcggtagt cataaaattg ctgggccacg ttatccgggc ctccttcaac gcaaggacca
    18301 ctgcgtgtgc cgttatcttt ataagctgga gtgactgtgg tgcaggcact gaggagcagt
    18361 gcgcagggga taagcattgt caatttgctg tagcgcataa tgatttcctt ataagcgatc
    18421 gctctgaaag cgttctacga taataatgat atcctttcaa taatagcgta tcagtctgat
    18481 aatgctttag agatcgaagg cttaacaaac aaggagatcg atcatgcaat tttctacaac
    18541 cccaactctg gaaggccaga tcatcgttga atattgcggt gtggtgaccg gcgaagcgat
    18601 tttaggtgcc aatattttcc gtgatttctt tgccggtatc cgcgatatcg ttggcggacg
    18661 ttccggtgcg tatgaaaaag aactgcgtaa agcacgggag atcgcctttg aggaattagg
    18721 ctcccaggcg cgggcgctgg gggccgatgc cgtcgtcggt attgatatcg actacgaaac
    18781 ggtcgggcaa aacggcagta tgctgatggt tagcgtcagc ggtacggcgg tgaaaacgcg
    18841 tcgatgagaa gagtcttctg gctgatcgct gccgctctgt tactggcagg gtgtgcaggc
    18901 gaaaaaggca ttgtcgagaa agagggatat cagcttgata cccgacgcca ggcgcaggcg
    18961 gcgtatccgc gcattaaagt gctggtgatc cactacaccg cagatgattt tgatagctcg
    19021 ctggcgacac tgaccgataa gcaggtcagc tcgcattatc tggtccctgc ggtaccaccg
    19081 cgatacaacg gtaaaccgcg catctggcaa ctggtgccgg aacaagaact ggcctggcat
    19141 gcggggatta gcgcctggcg cggggcaacg cgccttaacg acacctctat tggcattgag
    19201 ctggaaaacc gtggctggca aaaatcggcc ggagtgaaat attttgcccc gtttgaaccg
    19261 gcacagattc aggcgcttat tccactggcg aaagatatta ttgcccgtta tcacatcaag
    19321 ccggaaaacg tagtggcaca tgcggatatc gcaccgcagc gcaaagacga tccggggcca
    19381 ttatttccct ggcagcaact ggcgcagcag gggattggtg cctggccgga tgcgcagcgg
    19441 gttaactttt accttgccgg gcgcgcgccg cacacaccgg tagataccgc gtcattgctg
    19501 gagcttttgg cgcgctacgg ttatgacgtt aaacctgata tgacaccgcg cgagcagcgg
    19561 cgcgtgatta tggcattcca gatgcatttc cgcccgacgt tatataacgg cgaagcggat
    19621 gcagaaactc aggcgattgc cgaagcattg ctggagaaat acgggcagga ttagcgcggc
    19681 agttttccgt ggtcgcgtag ccaggcggca gttttctcga taccttcatc cagggtgatg
    19741 accggctgat aacctaactc ttcctgcgca cgcgtaatat ccagcgtaaa gtcaaaattc
    19801 aacttggaga cgccgtagtg ggtcagcggc ggctcttttg ctgacttgcg gcctaaacgc
    19861 tccatgctgc gggcgatcat atccagcatc gggtagggga cggaacgaat acgacagtca
    19921 atattcaact cgtcgatcag cttctgcacg atgctgcgca gtgtgcgatg ctcgccgttg
    19981 gtgatgttgt acacacgccc ggaaggtagc ttatcgcagg cttcctggct tgccagccac
    20041 attgcgtgca cggcattttc atagtaggtc atatcgacca gcgcactgcc gccatgcggt
    20101 aacagaatac tgccgtagtg gtgcatcata tgcgccagac ggggaataaa gactttatcg
    20161 tgcggtccga acagactttg tgggcgcaga atagtaaagc gcgtttgtgg attcgcctgc
    20221 gaaagcatat tgatcacttc ttcgctggct gctttgctgc gggcaaactc gttggcgaag
    20281 cggtgagggc gaaaatcttc tttaatatcg cgatggtggt gataatcgaa gtacagggag
    20341 ggggaagaga tatgaataaa gttacgcaca ccccaggcga cagtccattc acccaggcgg
    20401 cgagtggcgc gaacgttagc cagatcgaaa gcctgttgtg tcccccaggg tgaggtaaag
    20461 ctggagcagt gccacagcgt atcaatgccc gcgagcatca ctttagcttg tgatgaaacc
    20521 agctcggtca gatccgccgg aacaaactct gcgcccattt tttccagcaa tttgcccatt
    20581 gcctcgttgc gaccggtcgc tcgcacgctg atgcctttct ggcataaaaa ctctaccgcg
    20641 tttcgaccta agccgctggt ggcgccggta accagtacct tcatatcaat ccactgttgt
    20701 tgagaaaata acgtgcgcat tcttccgtga tttcccccat gatgcaatgg gaaacatgaa
    20761 agaataacgc aggttttgtc gattaatctg tgctttgttc tgccagtctg gcgatttgtt
    20821 ttgccattcc gcgaaaaata aacagatgcg cggggatcat caataaccag taaaacagcc
    20881 ccggcatacc gtgcggatgc cagaaagcgc ggacatcgat agtactatag tcgcctttat
    20941 cttccaggct aaaacacagt cgtcccagcc ccggcgcttt catgccaaat aacaacgtaa
    21001 gttgtttttc cggttcaacg acaatcactt tccagctatc caccgcatcg ccagtctgta
    21061 aatattcgcg ctccgggcgg cctttcgcca gcttatgacc gatcgcgcgg tccatcaacg
    21121 cccgtgtctg ccacaaaata ttgccaaaga aataacgctc tttaccgccg atttggttca
    21181 ctacctgcca taaagcagca aggctggcgg acgttttaac ggtaaacccc gcctgtttgg
    21241 caaaataacc gtactctggt cgccagcggg caaaggcctg agcgtcgtag ccccagtcgc
    21301 tggagttgac cagtttttcc tcctctttca acgtgctacg taccgcgtca tcgaaagcga
    21361 tcagccgttg tgggatgagt gcacgtagcg cggtatcatc cgccagcaga tcgtgtttca
    21421 gcccctgaat caacgccctg gcggtggtgg gcggtacgga agtaatcaca ttgagaaacc
    21481 acaccgaaat ccagcgggtg gggaggggga tggggatcaa ccagcggcgc ttaccgctca
    21541 ccgccataaa atgttcaaac tgttgctgat aactgagcac ctctggtccg gcggcttcga
    21601 agatgcggtg ttcgctggcc ggatgatcta acaacgccac cagatagtgc agcaagtttt
    21661 ccagcgcgat gggcgtggtg cgtgaacgta cccagcgtgg cggcgttaac actggcaggt
    21721 tgtagaccat atcgcgcatg acttcgaacg ccgctgaacc tgcgccaacg ataattccgg
    21781 cccgaagttc ggtcacaggt acattcgctt cacgaagaat gtccgccgta gcctgacgag
    21841 cacgcagatg atccgactgc tcatgtggcg gggcctgcaa cgaactgaga aagattaatt
    21901 gcttaactgg tacttcacgt agcgcatcgc ggacgttgag agccacctgg cgctcctgag
    21961 cgataaaatc gccgccttcg cccatgctgt gcaccagaaa atagacggta tcgatatcct
    22021 gcaacagggc cggaaggtta tccggccagc tgagatcgac tttatggcaa ctgacgtttg
    22081 ccagttgcag ctttgcaagc ctgtcgacat gacgtgccgc cgccaggatc tgatgccctt
    22141 gctggctgag tgtgcgcacc agatgctgac caatgtagcc actggcaccg agaactaaaa
    22201 tgcgttgcgg cacgtctctc tccttaacgc gccaggaatg cgcgccagtg ggcggcgact
    22261 tccgccagtt gttcgcgcga gacgtcaaga tgcgtcacca ggcggacaat cggcgaggcg
    22321 ttaatcagca cgtttctcgc tttcatgtat tcgcctaacg cggcagcatt ttcttccccg
    22381 acgcgaacaa acagcatatt agtgtcctga cgcatcacat ccgcgcctgc ttcacgcagt
    22441 tgctccgcca tccaggcggc gttgtcgtga tcttcctgca accgtgcgac gttatttttc
    22501 agggcataca tcccggcggc agccagaatc ccggactgac gcatcccgcc accggccatt
    22561 ttccgccagc ggatggcacg tttaatgtaa tcacgattac cgacgagtaa tgaaccgact
    22621 ggcgtcccaa gacctttcga caggcaaatg gtgaacgaat cacaatattg cgtgatctct
    22681 ttcagttcgc agccgtaagc caccacggca ttaaagatgc gcgcaccgtc aacatgcagc
    22741 gccagattgc gctcgcgggt aaattcccat gcttctttca ggtattcacg cggcagcact
    22801 ttgccgttgt gggtgttttc cagactgagt aatttggtgc gggcgaaatg gatatcgtcg
    22861 ggtttgattt tcatcgccac tttatccagc ggtagcgtgc cgtcggcagc cgcgtcgatg
    22921 ggttgcggct gaatactgcc cagcaccgct gcgccaccgg cttcaaacag atagttatgc
    22981 gcggcctgac cgacaatata ctcttcgcca cgttcgcagt gactgagcag agcgaccagg
    23041 ttggcctgag tgccggtagg cagaaaaatg gcggcttctt taccggaaag ctctgctgca
    23101 tagtcctgca gagcattaac ggtagggtcg tctccgtaaa cgtcgtcccc aaccggggcg
    23161 gccatcatcg cttcgagcat ggcgcggctc ggtcgggtaa cggtatcact gcgtaaatca
    23221 atcatggcat gtccttatta tgacgggaaa tgccaccctt tttaccttag ccagttcgtt
    23281 ttcgccagtt cgatcacttc atcgccgcgt ccgctgataa ttgcgcgcaa catatacagg
    23341 ctaaaacctt tggcctgttc gagtttgatc tgcggtggaa tggctaactc ttctttggcg
    23401 accaccacat ccaccaacac cggaccgtcg atagagaagg cgcgttgcag ggcttcatca
    23461 acttcagacg ctttttctac acggataccc gtaatgccgc acgcttcggc aatgcgggca
    23521 aagtttgtgt cgtgtagttc ggtgccgtca gtcaaatagc caccagcttt catctccatc
    23581 gccacaaagc ccagcacgct gttgttaaag acgacaattt tcactggcag tttcatctgc
    23641 actactgaga ggaaatcgcc catcaacatg ctaaaaccgc catcgccgca catggcgacc
    23701 acctgacgtt ctggctctgt cgcctgcgca cccagcgcct gcggcatggc gttagccatc
    23761 gaaccgtggt taaacgaacc taacaggcga cgcttgccgt tcatttttag ataacgtgcc
    23821 gcccacaccg ttggcgtacc aacgtcacag gtgaaaatag cgtcatcggc ggcaaaatga
    23881 ctaatttgct gcgccagata ttgcgggtga atggctttct cgctcggttt agctaaatcg
    23941 tccagccctt tgcgggcgtc gcggtaatct tccagcgctt tatccagaaa cttgcgatcg
    24001 gctttttctt ccaccaatgg aagcaatgca cgcagagtcg acttgatatc gccgaccagt
    24061 gccatatcca ccttgctgtg agcgccgatg ctggctgggt tgatatcaat ctgaatgatt
    24121 ttggcatcgg tcgggtagaa ggcgcggtag ggaaattgcg tgccgagtag cactaacgtg
    24181 tcggcgttca tcatggtatg gaaacctgac gagaagccga ttaacccggt cattccaaca
    24241 tcatacggat tatcgtattc gacatgttct ttaccgcgca gggcatgaac aataggcgct
    24301 ttaattttcc cggcaaactc aactaactct ttatgcgccc ccgcgcagcc gctgccacac
    24361 atcagggcga tattgctgga ataacgcagc agttgcgcca gtttgcgtaa ctcttcttct
    24421 tccggcgtca cgactggttg tggcgcatga taccagtgcg tggttgcccc ttctggcgca
    24481 ggttttaacg ccacgtcgcc tggtaacacg acaaccgaaa cgccacggtt aagcaccgct
    24541 ttgcgcatgg caatcgccag tacttgtggg atctgctccg ggctggaaac cagctcgcaa
    24601 tagtgactac attcgcggaa tagctcttgt gggtgggttt cctggaaata gccgctgcca
    24661 atttcgctgg agggaatatg agcggcaatc gccagtaccg gaacgtgatt gcggtggcaa
    24721 tcgaacaggc cgttgattaa gtgcaggttg ccggggccgc acgatccggc gcagaccgcc
    24781 agttctccgc taagttgtgc ttcagcgcca gcggcaaagg ccgccacttc ttcgtggcgg
    24841 gtggacatcc actcgatggt gcccatgcga ttaagactgt cactaagacc gttcagagag
    24901 tcgcctgtga ctccccagat gcgtttcacc cctgccgatt cgagtgtttt ggcgatataa
    24961 gctgcaaccg tttgtttcat ggttctccat ctcctgaatg tgataacggt aacaagttta
    25021 gttcatctga cggaggggga agggatggga gagaaaggag gcactaacgg ttaaatagcc
    25081 cgatgaaagg aatatcatcg ggcataaggc gattatgcga gaaccaaatc cccctgcgga
    25141 tggcaggagc aggccagtac gtaaccttca gcgatttcgg cgtcggtcag cgtcattgta
    25201 ctgctcaccg tatattcacc ggaaaccact tttgtcttac agcagccgca aacacccgca
    25261 cggcaggcag cgacaaccgg aacgttattg ctttccagcg cctccagtag cgtggtgcca
    25321 accggggcgt aaaattctcg tgccggttgc agtttggtga atttcagacc gctggtcgcc
    25381 gcttctgcta ctggggtgaa gaatttctct ttaaagaaac gcgtcacgcc gagcgctttc
    25441 acttcctgct ctacccaatc catatacgga gccgggccgc aggtcatcac ggtacgtgaa
    25501 gctaagtcag gtacacctgc cagcagttcg cgagtgagac gaccagcgat aaagccttcg
    25561 gtaacgttat tttctgccac cagcgttacc ggatagttac gccactcatc ggcgaaaata
    25621 acatcctgcg gcgtacgcac gttgtagatc acccgcacat cggcctgtgg acggttcttc
    25681 gcaagccagc gacgcatcga cataatcggc gtgacgccgc agcctgccgc cagcaacagg
    25741 aatttatctt ctgctttatc gtcgcaggta aattccccca tcgcgtccga aagccagaga
    25801 taatcaccgc gttttacatc gcgcgtcagc cactgggagc cgacaccgtc atcaatccgc
    25861 cgcacggtca gggtgatata ttcactcacg cctggcgtgg aggaaatggt gtaagcacgc
    25921 agcgtttccg ctgagtttcg cacgctgacc agtgcatatt gcccggcgcg atatgggtag
    25981 taatcgtggc aaatcaggga aatcgtccac acatccggcg tttcttgcgt aatgtgatga
    26041 acctgcatcc gccacgggca ttgattcgtt ggcatcgtca tcgacaaact ccttacgcgc
    26101 tcaacagttg cttcatgtct tcttcaacag tggtgataga acgcaggcca aatttctcgt
    26161 tcagcaccgc cagcaggtct ggtgtcagga aaccaggtgc agtcgggccg gtgacgatat
    26221 ttttcacgcc cagagaaagc agcgtcagca gaatgacgat cgctttctgt tcaaaccagg
    26281 agagcaccag cgacagcggc agatcgttga caccgcagcc cagtttctct gccagagtga
    26341 cagccagaat aatcgctgag taagcatcgt tacattgacc tgcatctacc agacgcggca
    26401 gaccttcgat atcgccaaac tcaagtttgt taaagcgata tttaccacag gcgagggtca
    26461 ggatcaggca gtcatccggc acgctggtgg cgaaatcggt gaagtagtgg cgctcgccgc
    26521 gtgcgccgtc acagccacca agcaggaaga tatgacgcag tttttcacgg ctcaccagat
    26581 caatcagcgt atcagcagcg ccaagcagcg tctggcgacc aaaacccacg gtgataaggt
    26641 gcggaatttc gctgtacggg aagcctgcca tctgttgcgc ctgggtgata accgcagaga
    26701 aatcatcacc gtccagatga cgcacgccag gccagccaac aatgctgcgg gtccagatac
    26761 gatcgtcata agcgcctacg gttgggtcga tgatgcagtt cgaggtcatc acgatggggc
    26821 cagggaaacg agcgaactcc acttgctgat tctgccagcc actgccgtag ttaccgacca
    26881 gatgcttgaa tttacgcagc tccgggtagc catgcgcagg cagcatttcg ccgtgggtgt
    26941 agacattaac gcccgtgcct tcggtctgtt ccagcaggtt gtagagatct ttgagatcgt
    27001 gaccggaaat cagaatgcat ttacccgccg tcgctttgac gttgacctgg gttggcgtcg
    27061 ggtgaccgta tttaccggtt tcgcctgcat ccagaatgct catcactttg aagttcatct
    27121 ggccgatttc cattgaacac tcaagaagcg cgttcatatc ggcaggccag gtccccagcc
    27181 acgccatgat tttatgatac tgggcataaa tatcgttgtc gtattgaccg agaacatgcg
    27241 cgtgttccat ataggccgcc gcacctttca ggccatacag gcacagcaga cgcaggccga
    27301 gaatgttttc gccaatcgcc gctttatctt tgttaggggt aaattctgca gcctgacgtt
    27361 gcagctcgcc gagatcgtcg ctcaccagtt gcaggtcagc catcgggtta tcgacgcgcg
    27421 cgttggcatc tacagccagg cattgcgctt tcagcgcctc gcgcagggca atcgcttcac
    27481 gagcgtagcc gacaatacgc ggagaatcga agttaacgtt ggtcagagtt gagaaaaagg
    27541 cacgtggcgc gaagctgtca acatcgtggt tgatgatgcc gtattcacgc gcttttaccg
    27601 cccaggcaga aagcccttgc agcgccgcga tgagtaaatc ctgaaggtca gaagtttccg
    27661 ccgttttacc acacatcccc tgcgcgtatg agcagccgtt tcctgccgga gtacggatag
    27721 tttgttcaca ttgcacacaa aacatgatca caccttttaa agttatattt aatatacatg
    27781 tttaaggtta agacgcttaa cgcggggata aaagggattt ttcatgcaac tttaagggag
    27841 attgatttag cgcaattttg gcggcaggaa tctaccgcca gagaggtatt acgcagagaa
    27901 aaaggcgatg aggatcggca ctaacaggct aagaataaaa ccgtgaacaa ttgccgccgg
    27961 gaccatatcc agcccgccag tacgttgaag aacgggcagg gtgaaatcca ttgatgtggc
    28021 accgcataag cccagtgcag tagagcggct gcggcgaatc agcccaggga tcaacataat
    28081 agcaatcagt tcacgggcca gatcattaaa aaacgccgcg ctcccgatta ccggaccaaa
    28141 agattcggtc aataaaatac cggaaagaga ataccagccg aaaccggagg ccattgccag
    28201 cgaggtattg atggggagat caagaataaa ggcgttaatt aaaccaccaa ttaatgaact
    28261 gacaaccacc accacggcga caatcattcc ccggcgatta aggacaatct gctttaaggt
    28321 catgccatta ttgcgcaact gaataccaac gaggaaaagt agcaaaatta acgtgtattc
    28381 actggcttcg gtcgcgtgtt gtaagaaagc cagtccactc agaccaatgg caaaaccaat
    28441 cactactacg ccgcacagtt ttagcgactc cagcgccatc gcaatacgcg acgggagttt
    28501 ttcttgctga tggtggttgc gccacggcag gcctcgctcc agccacatca gggcggcaat
    28561 attacacagt aaaataacgg taatactgac ggcagaataa tgcagaatcg ccaacaggtt
    28621 actggcgagg ttatcgagga acgccagact tatacccata aaaaagagaa taaggtaaac
    28681 catccagctt aatagctgat taataacttt taacgcagct tgttggcgaa gcggaatgag
    28741 gtaacccaca atcaggggaa ccagaatgat taacagccca gaaaacatga aaacccagtc
    28801 cttgcaaaga tgaagtcgaa atgcgcgatg acacactact gaaagcggaa ggacgagtaa
    28861 agttgcaatt aaaaggaaat gttatgcata aggagcagta gagtattcgt tttcatttaa
    28921 agatattctt gcgctttaat tacaaactgc accgatgttg gtggcgtcaa aatcgccgag
    28981 gcgttccctg aaggccgggg cagcccacat ggatgtgggc tgagggcgcg ttttacaggg
    29041 atgttacctc gcgcccgacc cggtagccgt aagggataag tcgagggcac cgcgcagcgg
    29101 cgattttgtt cgccagagcc cgggggtgca tggggcggcg gcgattggcc gccccctgcg
    29161 cgctccttgc gccagtggca atatgtggct tagctcatga aaggagcgca acaagatgat
    29221 gaatcaacat ataacaacat cttaaaaaaa ggcctgacat tacgccaggc cttctgcgtt
    29281 aattaatcac gcttttccag cagggtccgg taaatcagac caccgataat gccgccgaca
    29341 attggcacca cccagaagaa ccacagttgt tctaatgccc agccgccctg gaagatagca
    29401 accgcggtgc tgcgcgccgg gttaacagaa gtgttagtca ccggaatact aattaagtga
    29461 atcagggtta aggccagacc aatagcgatc ggcgcaaaac ctgccggcgc gaatttgtcg
    29521 gttgcgccgt ggatcaccaa caggaaacct gcactcaata ccagttcaac taccagcgcg
    29581 gaaagcatgg aataaccgcc tggtgaatgc tcgccataac cgttagaagc aaaaccgctg
    29641 gctgccgcgt caaaacccgt tttaccactg gcaattaaat acagcagcgc cgctgcaaca
    29701 ataccgccga caacctgggc aattacgtag ccaacgactt cttttgccgg aaaacgtccg
    29761 ccagcccata aaccaatagt gaccgccggg ttaaaatgac caccagaaat atgaccaaca
    29821 gcaaaggcca tcgtcagaac ggtcagaccg aacgccaacg ccacgccggc aaaaccaatg
    29881 cctaattccg ggaagcctgc ggccagtaca gcactaccac agccaccaaa aacaagccag
    29941 aaagtaccaa aacattcagc tgctaatttt ctgaacatat ccacctcaat taaaaattga
    30001 ccctgtgaaa aatatggtcg ttttataggg tcgtcgtaaa aaagtgacga cggaaataat
    30061 gcgcggctat tttaaaaacg aaggcgagtc attcaccaga taaataaatc cagtaaattt
    30121 gatttagggc aacagcgggt tgccccatat agtcatttgt ctgattgaca gtgtagtgca
    30181 agcaaaagat ttaatccttt aggcgtaata aaaaataatt tatcatgcta attatttgat
    30241 tttgttgttt ttgcagactt atcagcaaga gggagtataa cgcgattatt cgctcatttt
    30301 tcagacattt gccatgctta aatgtgatgt catcacgtat tagcaaggcc tttcccgtta
    30361 tactgccagc gtaaaggata agtcacatat ttctggaggg gatatgattc ttgagcgcgt
    30421 tgaaattgtg ggttttcgcg gtatcaaccg tttgtcgttg atgctcgaac aaaacaacgt
    30481 cctgattggg gagaacgcgt ggggtaaatc cagcttgctg gacgccttaa ccctgctgct
    30541 atcgccagaa tcagatctct accattttga gcgcgacgat ttctggttcc cgccgggaga
    30601 tatcaacggg cgagaacatc atctgcatat tattttgacc ttccgcgaat cgctgccagg
    30661 ccgacatcgg gttcgccgtt atcggccgct ggaagcgtgc tggacgccat gcaccgatgg
    30721 ctatcaccgt attttttatc gtctggaagg ggagagtgcg gaagacggca gcgtgatgac
    30781 actgcgcagt tttctcgata aagacggaca tccgattgat gtcgaggata ttaacgatca
    30841 ggcacgccat ctggtgcgtt taatgccggt gctgcgcttg cgtgatgccc gttttatgcg
    30901 ccgtattcgt aacggcacgg tgccaaatgt tcccaatgtg gaagtcaccg cgcgccagct
    30961 cgatttcctc gcccgtgagt tgtcctcaca tccgcaaaat ctctctgatg gacagattcg
    31021 tcagggactt tccgcaatgg tgcagttgct tgagcattat ttctctgagc agggggctgg
    31081 acaggcgcga tatcgtttaa tgcggcggcg ggccagcaat gagcaacgaa gctggcgcta
    31141 tctggacatc atcaaccgga tgattgaccg acctggaggg cgctcgtatc gggttatttt
    31201 gctcggcctg tttgctactt tattgcaggc aaaaggcaca ttgagactgg ataaagacgc
    31261 gcgtccgtta ttgctaattg aagatccaga aacccgttta caccccatta tgctttcagt
    31321 tgcctggcat ctgttgaatc ttctgccatt gcagcgcatt gccaccacca actcgggtga
    31381 gttgctttcg ttaacgccgg tagagcatgt ttgccgactg gtacgtgagt cctcgcgcgt
    31441 tgccgcctgg cgtctggggc cgagtggctt gagtaccgaa gatagccgac gcatatcctt
    31501 tcacattcgt tttaaccgtc cgtcatcgct gtttgctcgc tgctggttgc tggtggaagg
    31561 ggaaacggaa acctgggtta tcaatgaact ggcgcgccag tgtggacacc atttcgatgc
    31621 cgaagggatc aaggtcattg agtttgccca gtccgggcta aagccactgg ttaaatttgc
    31681 ccgccgaatg gggattgaat ggcatgtact ggtcgatggc gatgaagcag ggaagaaata
    31741 tgccgctacg gtacgcagcc tgttgaataa cgatcgggaa gccgaacgag aacatttaac
    31801 ggcgttaccg gcgctggata tggaacattt tatgtatcgc cagggatttt ccgatgtgtt
    31861 ccaccgcgtg gcgcaaatcc cggaaaatgt accgatgaat ctgcgcaaaa ttatctcgaa
    31921 agcgatccat agctcttcca aacccgatct tgccattgaa gtggcaatgg aggccggacg
    31981 tcgtggtgtg gactccgtac cgacgctgct gaaaaaaatg ttctcacgcg tgctgtggct
    32041 ggcgcgcggt cgcgcggatt aaccgcgaaa catcgtggcc atttgtggct gaatagcgtc
    32101 gagcatctca tagcgccgac ggtattcagc ccgtttttta ctggcgattt cggcaatctc
    32161 ttttcgtgct atctgtgctg gaaggcggta atggcgttca gcatcacata cgccgccaac
    32221 cgattcccag aaagcgttgt aatcggcatg gatcttgcct tctttatcac gataacgcag
    32281 gctgcggtaa atatgcgttt cattgctgac ggcaataatc tgctctacct gcaaacgttg
    32341 ggcaaacaga caggccgctt ccatcacgag gcgtttggga aatagcccgt ggcaggcttt
    32401 cgtcgcattc tggatttcct gatgtgggat ttcccatttt gcgccttgca gtccgccaat
    32461 aaacatcgtt cttttcccct gatattcaca cagggtaaaa gtgagctctg ccagaggaat
    32521 accttcgctg ttgcggaaca ggattgtgct gtcaccttct ttatccattg agatcatcat
    32581 ggtcagctca agcgtgaact gctcgccgtt tttgccttcc agtttagcca gttgcagccc
    32641 gggggtattc aaatataagc tgaattcttc cgccgacata catccgcgga gtaacgcata
    32701 atggtaacgt aacgcctcca gcaattgctt acggctaaga ttcgccgcaa ggtaagggcg
    32761 atgcagacgc acaggcagtc gcggttggcg cgttaacaac acattgagat taggccagtg
    32821 ggaaagttcg ttcatccact caacgcttaa acgcggcata atcaacgagc gcagcaaaaa
    32881 tttctggcga aaactacggc gatgccagaa tttacccggc cgacactgtc cacgtgccag
    32941 actaagaaaa agtgacaggc tgctgagaga ttcagatggc gtaaaggtcc gttcagttag
    33001 ctgcgacata ttcatgaaat caatggttat acatgacgtc gatttcacca ttgcgtatct
    33061 taaccaaaca tcaatagtgt gattactaac gtaaatttta gggttttgtt gatatttcgt
    33121 tgaagttaat gacccggatt ggcatatgga gtattcagaa tatttatgaa aaagcggaaa
    33181 accgtgaaga agcgttacgt tattgcgctg gtgatagtca tcgccggact gattacgtta
    33241 tggagaattc ttaacgcacc cgtgccgact tatcagacac tgattgtgcg ccccggtgat
    33301 ttacagcaaa gcgtgctggc gaccggaaag ctggacgcgc tgcgtaaggt tgacgtgggc
    33361 gcgcaggtca gcggtcagtt gaaaactctg tcggtggcga ttggcgataa agtaaaaaaa
    33421 gaccagcttt taggggttat tgatcctgaa caggctgaaa accagattaa ggaggtcgaa
    33481 gcaacgctga tggagctacg tgcgcagcgg cagcaggcgg aagcggagct gaaactggcg
    33541 cgggtgacgt attcccgtca gcaacgtctg gcacaaacgc aggcggtttc acagcaggat
    33601 ctcgacaccg ccgcgacgga gatggctgtg aaacaggcgc aaattggcac cattgacgcg
    33661 caaatcaagc gcaatcaggc ctctctcgat acggctaaaa ccaatctcga ttacactcgc
    33721 atcgttgccc cgatggccgg ggaagtcacg caaatcacca ctctgcaagg ccagacggtg
    33781 attgccgcac aacaagcacc gaacattctg acgctggcag atatgagcac catgctggta
    33841 aaagcgcagg tttctgaagc ggatgtaatc cacctgaagc cggggcaaaa agcctggttt
    33901 acggtacttg gcgatccact gacgcgctac gaggggcaaa tcaaggatgt actaccgacg
    33961 ccggaaaagg ttaacgacgc tattttctat tacgcccgtt ttgaagtccc caaccccaat
    34021 ggtttgctgc ggctggatat gactgcgcaa gtgcatattc agctcaccga tgtgaaaaat
    34081 gtgctgacga tccctctgtc ggcgttaggc gatccggttg gcgataatcg ttataaagtc
    34141 aaattgttgc gtaatggtga aacacgcgag cgtgaagtga cgattggcgc acgtaacgat
    34201 accgatgttg agattgtcaa agggcttgaa gcgggcgatg aagtggtgat tggtgaggcc
    34261 aaaccaggag ctgcacaatg acgcctttgc tcgaattaaa ggatattcgt cgcagctatc
    34321 ctgccggtga tgagcaggtt gaggtgctga agggcatcac cctcgatatt tatgccggtg
    34381 agatggtagc gattgttggc gcttcaggtt ccggtaaatc gaccctgatg aatattctcg
    34441 gctgtctgga taaggccacc agcggcacct atcgcgtcgc cggtcaggat gttgccacac
    34501 tggacgccga tgcgctggcg caactgcgcc gcgagcattt cggctttatt ttccagcgtt
    34561 accatttact ttcgcattta accgccgagc agaacgttga agtacccgcc gtctatgctg
    34621 gtcttgagcg gaaacagcga ctgcttcgtg cccaggagtt gctgcaacgg ctggggctgg
    34681 aagaccgtac agagtattat ccggcacagc tttcgggtgg tcagcaacag cgcgtcagca
    34741 tcgcgcgggc attgatgaac ggtggtcagg taattcttgc cgatgaacca accggcgcac
    34801 tggacagcca ttctggcgaa gaggtgatgg cgatcctgca tcagctgcgc gatcgtgggc
    34861 atacggtgat tatcgtcacc cacgatccgc aggtcgctgc tcaggccgag cgggtgatcg
    34921 aaattcgcga cggcgaaatt gtgcgcaatc ctcccgccat tgaaaaagtg aatgttgctg
    34981 gcgggacgga gcctgttgtc aacacggtgt ctggctggcg gcagtttgtc agcggtttta
    35041 acgaggcgct gacgatggca tggcgggcgc tggcagcgaa taaaatgcgt actttactga
    35101 ccatgctggg gattattatc ggtattgcgt cggtggtttc cattgtcgtg gtgggtgacg
    35161 ccgccaaaca aatggtgctg gcggatattc gttctattgg tacgaatact attgatgtct
    35221 atcccgggaa agattttggc gatgacgatc cgcaatatca gcaggcgctg aagtacgacg
    35281 acttaatcgc catccaaaaa caaccgtggg tcgcctcagc cacacctgcc gtctcgcaaa
    35341 acctgcgcct gcgttataac aatgttgatg ttgctgccag tgccaatggc gtgagcggcg
    35401 attattttaa tgtctatggc atgaccttca gtgaaggaaa cacctttaat caggagcagc
    35461 tgaacggtcg tgcgcaggtc gtggttctcg acagtaatac tcgccgccag cttttccccc
    35521 ataaagcaga tgtggttggc gaggtgattc tggtcggcaa tatgcccgcc agagtcattg
    35581 gtgtggcgga agaaaaacag tcgatgtttg gtagcagtaa agtgctgcgt gtctggctac
    35641 cttacagcac gatgtccggg cgagttatgg gccagtcgtg gctgaactcc attactgtca
    35701 gggtaaaaga aggatttgac agcgccgagg cggaacagca actcacgcgt ttactttcac
    35761 tgcgccacgg aaagaaggat ttctttacct ggaacatgga cggcgtcttg aaaactgttg
    35821 aaaagaccac acgtacttta caactgtttc tgacgctggt ggcggtgatt tcgctggtgg
    35881 tgggcggtat tggtgtaatg aatattatgc tggtgtcagt gaccgagcgg acgcgggaaa
    35941 ttggcattcg catggctgta ggtgcgcgag caagcgatgt tttgcaacag ttcctgatcg
    36001 aagccgtact ggtttgcctg gtcggtggcg cgttgggaat aacactgtca ctgttaattg
    36061 ctttcacctt gcagcttttc ttacccggct gggagattgg tttttcaccg ttggcactgc
    36121 tgctggcgtt tctctgctcg acggtcaccg ggattttatt tggatggtta cccgcacgaa
    36181 atgcggcacg actggatcca gtagatgctc tggcacgaga gtaatttttg agataaaaat
    36241 gccagccgat cgggctggca ttttgccttt aggatgtaca caatgagaca gaagagctat
    36301 gcgactgccg cttctacttc gacgggcaca ataacactgg cgtgattgcc ttttggcccc
    36361 tggtggacat caaactgaac ggattgtcca gcttttagcg ttctgtaacc atccatctga
    36421 atggtggaat aatgagcgaa aatatcttcg ccgccgcctt cagggcagat gaaaccaaac
    36481 cctttggcat tgttgaacca cttaacagta cccttttcca tgcttcgaca tccttcgcaa
    36541 atcttataca agtaagatgg aataaaccgg ggtcagagag ggggctgttc aaaacctcgc
    36601 caactctaga aatacaattt agagaattag ggcgagccgt caagcgtttg acaggggcca
    36661 gggggcaggt atgaatcaaa aatttgaagc agttaacgct attgacagga atgtgacaga
    36721 tgtcgctgat gccaacgata gatgatagtt atctatcatg tggagtagat tggtcaggca
    36781 aataagctct tgtcagcggc agggcgttct gccgataacc gtaaccgaag atgataactg
    36841 acaatgggta aaacgaacga ctggctggac tttgatcaac tggcggaaga aaaagttcgc
    36901 gacgcgctaa aaccgccatc tatgtataaa gtgatattag tcaatgatga ttacactccg
    36961 atggagtttg ttattgacgt gttacaaaaa ttcttttctt atgatgtaga acgtgcaacg
    37021 caattgatgc tcgctgttca ctaccagggg aaggccattt gcggagtctt taccgccgag
    37081 gttgcagaaa ccaaagtggc gatggtgaac aagtacgcga gggagaatga gcatccattg
    37141 ctgtgtacgc tagaaaaagc ctgaatgcag gcataaaaat tgggggaggt gcctatgctc
    37201 aatcaagaac tggaactcag tttaaatatg gctttcgcca gagcgcgcga gcaccgtcat
    37261 gagtttatga ccgtcgagca cttgttactg gcgctgctca gtaacccatc tgcccgggag
    37321 gcgctggaag cgtgttctgt ggatttggtt gcgctccgtc aggaactgga agcctttatt
    37381 gaacaaacca cacccgttct gcctgccagt gaagaggagc gcgacacaca gccgacgctg
    37441 agttttcagc gtgtactgca acgtgcggtc ttccatgtcc agtcctccgg tcgcaatgag
    37501 gtaaccggtg caaacgttct ggtcgctatc tttagcgaac aggagtcgca ggcggcatat
    37561 ctgttgcgta aacatgaagt cagccgtctc gatgtggtga actttatctc tcatggcacg
    37621 cgtaaagacg agccgacaca gtcttctgat cctggcagcc agccaaacag cgaagaacaa
    37681 gctggtgggg aggaacgtat ggagaatttc acgacgaacc tgaatcagct tgcgcgcgtg
    37741 ggcggaatcg acccactgat tggtcgtgag aaggagctgg agcgtgctat tcaggttctc
    37801 tgccgtcgcc gtaaaaacaa cccgctgctg gtgggggaat ctggtgtcgg taaaaccgcg
    37861 attgcggaag gtcttgcctg gcgaattgtt cagggcgatg tgccggaagt gatggctgac
    37921 tgtacgattt actctctcga tatcggttct ctgttagcgg gcactaaata tcgcggcgac
    37981 tttgaaaaac gttttaaagc gttgctcaag cagctggagc aggacactaa cagcatcctg
    38041 tttattgatg agatccacac cattatcggt gcgggtgcag cgtctggtgg ccaggtcgat
    38101 gcggctaacc tgatcaaacc gttgctctcc agcggtaaaa ttcgcgtaat tggttcgaca
    38161 acctatcagg agttcagcaa cattttcgag aaagaccgtg ctctggcgcg tcgcttccag
    38221 aaaattgata ttactgaacc gtcgatcgaa gaaactgttc aaatcatcaa tggcctgaaa
    38281 ccgaagtatg aagcgcacca cgacgtgcgt tataccgcaa aagcggtgcg tgcagcggta
    38341 gagctggcgg tgaaatacat taacgatcgt catctgccgg ataaagccat tgacgttatc
    38401 gacgaagcgg gcgctcgcgc acgcctgatg ccggtaagca aacgcaagaa aaccgttaat
    38461 gtggcggata ttgagtccgt ggtggcccgt attgcgcgca ttccagagaa gagtgtttct
    38521 cagagtgacc gcgataccct gaaaaacctc ggcgatcgcc tgaaaatgct ggtcttcggt
    38581 caggataaag ccattgaggc gctgactgaa gccattaaga tggcgcgtgc aggtttaggt
    38641 cacgaacata aaccggttgg ttcgttcctg tttgccggcc ctaccggggt cgggaaaaca
    38701 gaggtgacgg tacagctttc gaaagcgttg ggcattgagc tgctgcgctt tgatatgtcc
    38761 gagtatatgg aacgccatac cgtcagccgt ctgattggtg cgcctccggg atacgttggt
    38821 tttgatcagg gaggtttgct gactgatgcg gtcatcaagc atccacatgc ggtgctgttg
    38881 ctggacgaaa tcgagaaagc gcatccggac gtgttcaata ttctgttgca ggtgatggac
    38941 aacggtacgc tgaccgataa caacggacgc aaagcggact tccgtaacgt ggtgctggtg
    39001 atgaccacca acgccggggt acgtgaaact gagcgtaaat ccattggtct tatccaccag
    39061 gataacagca ccgatgcgat ggaggagatc aagaagatct ttacaccgga attccgtaac
    39121 cgtctcgaca acattatctg gtttgatcat ctgtcaaccg acgtgatcca tcaggtggtg
    39181 gataaattca tcgtcgagtt gcaggttcag ctggatcaga aaggtgtttc tctggaagtg
    39241 agccaggaag cgcgtaactg gctggccgag aaaggttacg accgggcaat gggcgcacgt
    39301 ccgatggcgc gtgtcatcca ggacaacctg aaaaaaccgc tcgccaacga actgctgttt
    39361 ggttcgctgg tggacggcgg tcaggtcacc gtcgcgctgg ataaagagaa aaatgagctg
    39421 acttacggat tccagagtgc acaaaagcac aaggcggaag cagcgcatta atctgattgt
    39481 taggtaggtt ggtcaagtcc gtaatctcga aagaggttac ggactttttg tttatggggt
    39541 ggaggaggtt cagacccttt tttaatgatg atggtaagtt gttgataatt agtgctgcgg
    39601 gaaggtaagg ataaaaaagg gtgctgcagg agaatgggat ggttttgctt tattaacaac
    39661 gggctaaacg tgtagtattt gagttcactg ccgtacaggc agcttagaaa tcgacggggt
    39721 gcggtaaaac ctttgcgaac gcgttcactg ccgtacagac agataaaatg cgaaaaaaaa
    39781 gctcgcactt tcgtacgagc tcttctttaa atatggcggt gagggggacc gcc
//
LOCUS       DAZGJW010000028        39014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZGJW010000028 DAZGJW010000000
VERSION     DAZGJW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.028"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            101..451
                     /gene="hypA"
                     /locus_tag="ACHKIK_001511"
     CDS             101..451
                     /gene="hypA"
                     /locus_tag="ACHKIK_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO9082711.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            455..1327
                     /gene="hypB"
                     /locus_tag="ACHKIK_001512"
     CDS             455..1327
                     /gene="hypB"
                     /locus_tag="ACHKIK_001512"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO9082712.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCLACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            1318..1590
                     /gene="hypC"
                     /locus_tag="ACHKIK_001513"
     CDS             1318..1590
                     /gene="hypC"
                     /locus_tag="ACHKIK_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO9082713.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            1590..2711
                     /gene="hypD"
                     /locus_tag="ACHKIK_001514"
     CDS             1590..2711
                     /gene="hypD"
                     /locus_tag="ACHKIK_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO9082714.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            2708..3718
                     /gene="hypE"
                     /locus_tag="ACHKIK_001515"
     CDS             2708..3718
                     /gene="hypE"
                     /locus_tag="ACHKIK_001515"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO9082715.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            3792..5870
                     /gene="flhA"
                     /locus_tag="ACHKIK_001516"
     CDS             3792..5870
                     /gene="flhA"
                     /locus_tag="ACHKIK_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO9082716.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRRILSRPDTLHCSYEEFC
                     ETWPQLVAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(5907..6260)
                     /locus_tag="ACHKIK_001517"
     CDS             complement(5907..6260)
                     /locus_tag="ACHKIK_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO9082717.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(6337..6471)
                     /locus_tag="ACHKIK_001518"
     CDS             complement(6337..6471)
                     /locus_tag="ACHKIK_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9082718.1"
                     /translation="MIPELIISGEEANCEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            6547..9108
                     /gene="mutS"
                     /locus_tag="ACHKIK_001519"
     CDS             6547..9108
                     /gene="mutS"
                     /locus_tag="ACHKIK_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO9082719.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            9214..9870
                     /gene="pphB"
                     /locus_tag="ACHKIK_001520"
     CDS             9214..9870
                     /gene="pphB"
                     /locus_tag="ACHKIK_001520"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO9082720.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(9921..10688)
                     /gene="ygbI"
                     /locus_tag="ACHKIK_001521"
     CDS             complement(9921..10688)
                     /gene="ygbI"
                     /locus_tag="ACHKIK_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO9082721.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            10884..11792
                     /gene="ltnD"
                     /locus_tag="ACHKIK_001522"
     CDS             10884..11792
                     /gene="ltnD"
                     /locus_tag="ACHKIK_001522"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO9082722.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            11789..13051
                     /gene="otnK"
                     /locus_tag="ACHKIK_001523"
     CDS             11789..13051
                     /gene="otnK"
                     /locus_tag="ACHKIK_001523"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO9082723.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            13048..13686
                     /locus_tag="ACHKIK_001524"
     CDS             13048..13686
                     /locus_tag="ACHKIK_001524"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO9082724.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            13691..14467
                     /locus_tag="ACHKIK_001525"
     CDS             13691..14467
                     /locus_tag="ACHKIK_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO9082725.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            14556..15920
                     /locus_tag="ACHKIK_001526"
     CDS             14556..15920
                     /locus_tag="ACHKIK_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO9082726.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(15959..16195)
                     /locus_tag="ACHKIK_001527"
     CDS             complement(15959..16195)
                     /locus_tag="ACHKIK_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO9082727.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTESLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(16206..17633)
                     /locus_tag="ACHKIK_001528"
     CDS             complement(16206..17633)
                     /locus_tag="ACHKIK_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO9082728.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(17633..18226)
                     /locus_tag="ACHKIK_001529"
     CDS             complement(17633..18226)
                     /locus_tag="ACHKIK_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO9082729.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            18373..18780
                     /locus_tag="ACHKIK_001530"
     CDS             18373..18780
                     /locus_tag="ACHKIK_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO9082730.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(18901..19893)
                     /gene="rpoS"
                     /locus_tag="ACHKIK_001531"
     CDS             complement(18901..19893)
                     /gene="rpoS"
                     /locus_tag="ACHKIK_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO9082731.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(19956..21050)
                     /gene="nlpD"
                     /locus_tag="ACHKIK_001532"
     CDS             complement(19956..21050)
                     /gene="nlpD"
                     /locus_tag="ACHKIK_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO9082732.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(21235..21861)
                     /gene="pcm"
                     /locus_tag="ACHKIK_001533"
     CDS             complement(21235..21861)
                     /gene="pcm"
                     /locus_tag="ACHKIK_001533"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO9082733.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(21855..22616)
                     /gene="surE"
                     /locus_tag="ACHKIK_001534"
     CDS             complement(21855..22616)
                     /gene="surE"
                     /locus_tag="ACHKIK_001534"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO9082734.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(22597..23646)
                     /gene="truD"
                     /locus_tag="ACHKIK_001535"
     CDS             complement(22597..23646)
                     /gene="truD"
                     /locus_tag="ACHKIK_001535"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO9082735.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLNDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(23643..24122)
                     /gene="ispF"
                     /locus_tag="ACHKIK_001536"
     CDS             complement(23643..24122)
                     /gene="ispF"
                     /locus_tag="ACHKIK_001536"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO9082736.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(24122..24832)
                     /gene="ispD"
                     /locus_tag="ACHKIK_001537"
     CDS             complement(24122..24832)
                     /gene="ispD"
                     /locus_tag="ACHKIK_001537"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO9082737.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(24851..25162)
                     /gene="ftsB"
                     /locus_tag="ACHKIK_001538"
     CDS             complement(24851..25162)
                     /gene="ftsB"
                     /locus_tag="ACHKIK_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO9082738.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(25356..25679)
                     /locus_tag="ACHKIK_001539"
     CDS             complement(25356..25679)
                     /locus_tag="ACHKIK_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO9082739.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(25729..26334)
                     /gene="cysC"
                     /locus_tag="ACHKIK_001540"
     CDS             complement(25729..26334)
                     /gene="cysC"
                     /locus_tag="ACHKIK_001540"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO9082740.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(26334..27761)
                     /gene="cysN"
                     /locus_tag="ACHKIK_001541"
     CDS             complement(26334..27761)
                     /gene="cysN"
                     /locus_tag="ACHKIK_001541"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO9082741.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGS
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(27763..28671)
                     /gene="cysD"
                     /locus_tag="ACHKIK_001542"
     CDS             complement(27763..28671)
                     /gene="cysD"
                     /locus_tag="ACHKIK_001542"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO9082742.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            28923..29960
                     /gene="iap"
                     /locus_tag="ACHKIK_001543"
     CDS             28923..29960
                     /gene="iap"
                     /locus_tag="ACHKIK_001543"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO9082743.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   30042..30375
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=30103..30131
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(30472..30765)
                     /gene="cas2e"
                     /locus_tag="ACHKIK_001544"
     CDS             complement(30472..30765)
                     /gene="cas2e"
                     /locus_tag="ACHKIK_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO9082744.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(30765..31685)
                     /gene="cas1e"
                     /locus_tag="ACHKIK_001545"
     CDS             complement(30765..31685)
                     /gene="cas1e"
                     /locus_tag="ACHKIK_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO9082745.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(31682..32332)
                     /gene="cas6e"
                     /locus_tag="ACHKIK_001546"
     CDS             complement(31682..32332)
                     /gene="cas6e"
                     /locus_tag="ACHKIK_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO9082746.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGPAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(32314..33060)
                     /gene="cas5e"
                     /locus_tag="ACHKIK_001547"
     CDS             complement(32314..33060)
                     /gene="cas5e"
                     /locus_tag="ACHKIK_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO9082747.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(33071..34126)
                     /gene="cas7e"
                     /locus_tag="ACHKIK_001548"
     CDS             complement(33071..34126)
                     /gene="cas7e"
                     /locus_tag="ACHKIK_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO9082748.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWSAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(34138..34677)
                     /gene="casB"
                     /locus_tag="ACHKIK_001549"
                     /gene_synonym="cse2"
     CDS             complement(34138..34677)
                     /gene="casB"
                     /locus_tag="ACHKIK_001549"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO9082749.1"
                     /translation="MSIVKEEHKATLRKWREELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSKGRFTRL
                     SAVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQNQ"
     gene            complement(34674..36236)
                     /gene="casA"
                     /locus_tag="ACHKIK_001550"
                     /gene_synonym="cse1"
     CDS             complement(34674..36236)
                     /gene="casA"
                     /locus_tag="ACHKIK_001550"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO9082750.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKNHGRWDDIWQDGLEAEKLREALLSLEHAFQFGADSPSFM
                     QDFEALKGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWPNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRIPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLKKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(36334..>39014)
                     /gene="cas3"
                     /locus_tag="ACHKIK_001551"
     CDS             complement(36334..>39014)
                     /gene="cas3"
                     /locus_tag="ACHKIK_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFO9082751.1"
                     /translation="LLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYL
                     MVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQEG
                     ARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGQPP
                     DRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYISATVV
                     LADWTGSSTRFFPRVAHPMDIKGYWQKTLIQAQNALTVFPLKAKVAPFNGINTLFPFI
                     ENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLFFGLP
                     TMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDE
                     QTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEI
                     HACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQQEAPFLE
                     KDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWI
                     RNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKYCSLQRAS
                     QVIVCTQVIEQSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSP
                     PELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHAARLL
                     IESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLST
                     RLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRL
                     WCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT         9212 a        10529 c         9972 g         9301 t
ORIGIN      
        1 tcgacgtgtc atttcgacat catcgacatt attcaccgca gggataatca acactggcac
       61 aattattgct tgtagctggc aatagttaat gggaggcgat atgcacgaaa taaccctctg
      121 ccaacgggca ctggaattga tcgaacagca ggccgcaaaa cacggcgcaa aacgcgtaac
      181 tggggtctgg ctcaaaattg gcgcattttc ttgtgtcgaa accagctctc ttgccttttg
      241 ttttgatctg gtttgccgcg gcagcgtggc ggaaggttgt aaactgcacc tcgaagaaca
      301 agaggccgaa tgctggtgtg aaacatgcca acagtatgtg acgctactga cccagcgcgt
      361 ccgccgctgt ccacagtgtc atggtgacat gctgcagatt gtggcagacg acggtttaca
      421 gattcggcgg atagaaatag accaggagtg agcgatgtgt acaacatgcg gttgcggtga
      481 aggcaacctg tatatcgagg gtgatgaaca taaccctcat tccgcgtttc gtagcgcgcc
      541 atttgccccg gcggcacgcc cgaagatgaa aatcaccggc attaaagcgc ctgaatttac
      601 ccccagccag actgaagaag gcgacctgca ttacggtcat ggcgaagcgg gcactcacgc
      661 accgggcatg agccagcgtc ggatgctgga agtcgaaatt gacgtgctgg acaaaaataa
      721 ccgtctggct gaacgcaacc gcgcgcgctt tgctgcccgc aagcaactgg tgctcaacct
      781 ggtttccagc ccaggttccg gtaaaaccac cctgctgacg gaaaccttaa tgcgcctgaa
      841 agacagcgtt ccgtgcgcgg ttattgaagg tgaccagcaa accgtgaacg atgccgcacg
      901 cattcgcgct accggcacac cagcgattca ggtgaacacc ggtaaaggct gccatcttga
      961 cgcacagatg attgccgacg ccgcaccgcg tctgccactg gacgataacg gtattctgtt
     1021 tatcgaaaac gttggcaacc tcgtatgccc ggccagcttc gatctcggtg aaaaacacaa
     1081 agtggcggtg ctttccgtta ccgaaggtga agacaaaccg ctgaaatatc cgcatatgtt
     1141 tgccgccgcc tcgctgatgc tgctcaacaa agttgacctg ttgccgtatc tcaactttga
     1201 cgttgagaag tgcctcgcct gcgcccgcga agtcaatcca gaaattgaaa tcatccttat
     1261 ttccgccacc agcggtgaag ggatggacca gtggctgaac tggctggaga cacagcgatg
     1321 tgcataggcg ttcccggcca gatccgcacc attgacggca accaggcaaa agtcgacgtc
     1381 tgcggcattc agcgcgatgt cgatttaacg ttagtcggca gctgcgatga aaacggtcag
     1441 ccgcgcgtgg gccagtgggt actggtacac gttggctttg ccatgagcgt aattaatgaa
     1501 gccgaagcac gcgacactct cgacgcctta caaaacatgt ttgacgttga gccggatgtc
     1561 ggcgcgctgt tgtatggcga ggaaaaataa tgcgttttgt tgatgaatat cgcgcgccgg
     1621 aacaggtgat gcagttaatt gagcatctgc gcgaacgtgc ttcacatctc tcttacaccg
     1681 ccgaacgccc tctgcggatt atggaagtgt gcggtggtca tacccacgct atttttaaat
     1741 tcggcctcga ccagttactg cctgaaaacg ttgagtttat ccacggtccg ggttgcccgg
     1801 tgtgcgtact gccgatgggt agaatcgaca cctgcgtgga gattgccagc catccggaag
     1861 tcatcttctg tacctttggc gacgccatgc gcgtaccagg gaaacaggga tcgctgttgc
     1921 aggcaaaagc acgcggtgcc gatgtgcgca tcgtttactc gccgatggat gcgttgaaac
     1981 tggcgcagga gaatccaacc cgcaaagtgg tgttcttcgg cttaggtttt gaaaccacca
     2041 tgccgaccac cgccatcact ctgcaacagg cgaaagcgcg tgatgtgcag aatttttact
     2101 tcttctgcca gcacattacg cttatcccga cgctgcgcag tttgctggaa cagccggata
     2161 acggtatcga cgcgttcctc gcgccgggcc acgttagtat ggttatcggc actgatgcct
     2221 ataattttat cgccagcgat tttcatcgtc cgctggtggt cgctggtttc gaaccccttg
     2281 atctactgca aggcgtggtc atgctggtgg agcagaaaat agcggcccac agcaaggtag
     2341 agaatcagta tcgtcgggtg gtaccggatg ccggtaacct gctggcgcaa caggcgattg
     2401 ccgatgtgtt ctgtgtcaac ggcgacagcg aatggcgcgg cttaggcgtg attgaatctt
     2461 ctggcgtgca cctgacgccg gattatcaac gattcgatgc cgaagcacat ttccgcccgg
     2521 caccgcagca ggtctgcgat gacccgcgcg cgcgttgtgg tgaggtatta acgggcaaat
     2581 gtaagccgca tcaatgcccg ctgtttggta acacctgtaa tcctcaaacc gcatttggtg
     2641 cactgatggt ctcctccgaa ggagcgtgcg ccgcgtggta tcagtatcgt cagcaggaga
     2701 gtgaagcgtg aataatatcc aactcgccca cggcagcggc ggccaggcga tgcagcaatt
     2761 aatcaacagc ctgtttatgg aagcctttgc caacccgtgg ctggcagagc aggaagatca
     2821 ggcacgtctt gatctggcgc agctggtagc agaaggcgat cgtctggcgt tctccaccga
     2881 cagttacgtc attgacccgc tgttctttcc tggcggtaat atcggcaagc tggcgatttg
     2941 cggcactgcg aatgacgttg cggtcagtgg cgctattccg cgctatctct cctgtggctt
     3001 tatcctcgaa gaaggattgc cgatggagac actgaaagcc gtagtgacca gcatggcaga
     3061 aaccgcccgc acggcaggca ttgccatcgt tactggcgat actaaagtgg tgcagcgcgg
     3121 cgcggcagat aaactgttta tcaacaccgc tggcatgggc gcaattccgg cgaatattca
     3181 ctggggcgca cagacgctaa ccgcaggcga tgtattactg gttagcggca cgctcggcga
     3241 ccacggggcg actatcctta acctgcgtga gcagctgggg ctggatggcg aactggtcag
     3301 cgactgcgcg gtactgacgc cgcttattca gacgctgcgt gacattcccg gcgtgaaagc
     3361 gctgcgtgat gccacccgtg gtggtgtgaa cgcggtagtt catgagttcg cggcagcctg
     3421 cggttgtggt attgaaattt cagaagcggc actgccggtt aaacctgccg tgcgcggcgt
     3481 ttgcgaactg ctgggactgg acgcccttaa ctttgccaac gaaggcaaac tggtgatcgc
     3541 cgttgaacgc aacgcggcag agcaagtgct ggcagcgtta cattcccatc cactgggaaa
     3601 agacgcggcg ctgattggtg aagtggtgga acgtaaaggt gttcgtcttg ccggtctgta
     3661 tggcgtgaaa cgaaccctcg atttaccaca cgccgaaccg cttccgcgta tatgctaata
     3721 aaattctaaa tctcctatag ttagtcaatg accttttgca ccgctttgcg gtgctttcct
     3781 ggaagaacaa aatgtcatat acaccgatga gtgatctcgg acagcaaggg ttgttcgaca
     3841 tcactcggac actattgcag cagcccgatc tggcctcgct gtgtgaggct ctttcgcaac
     3901 tggtaaagcg ttctgcgctc gccgacaacg cggctattgt gttgtggcaa gcgcagactc
     3961 aacgtgcgtc ttattacgca tcgcgtgaaa aagacacccc cattaaatat gaagacgaaa
     4021 ctgttctggc acacggtccg gtacgccgca ttttgtcgcg ccctgatacg ctgcattgca
     4081 gttacgaaga attttgtgaa acctggccgc agctggtcgc aggtgggcta taccccaaat
     4141 ttggtcacta ttgcctgatg ccactggcgg cggaagggca tatttttggt ggctgtgaat
     4201 ttattcgtta tgacgatcgt ccctggagcg aaaaagagtt caatcgtctg caaacattta
     4261 cgcagatcgt ttctgtcgtc accgaacaaa tccagagccg cgtcgtcaac aatgtcgact
     4321 atgagttgtt atgccgggag cgcgataact tccgcatcct ggttgccatt accaacgcgg
     4381 tgctttcccg cctggatatg gacgaactgg tcagcgaagt cgccaaagaa atccattact
     4441 atttcgacat tgacgatatc agcattgtct tacgcagcca ccgtaaaaac aaactcaaca
     4501 tctactccac tcactatctt gataaacaac atcccgccca cgaacagagc gaagtcgatg
     4561 aagccggaac cctcaccgaa cgcgtgttca aaagtaaaga gatgctgctg atcaatctcc
     4621 acgagcggga cgatttagcc ccctatgaac gcatgttgtt cgacacctgg ggcaaccaga
     4681 ttcaaacctt gtgcctgtta ccgctgatgt ctggcgacac catgctgggc gtgctgaaac
     4741 tggcgcaatg cgaagagaaa gtgtttacca ctaccaatct gaatttgctg cgccagattg
     4801 ccgaacgtgt ggcaatcgct gtcgataacg ccctcgccta tcaggaaatc catcgtctga
     4861 aagaacggct ggttgatgaa aacctcgccc tgaccgagca gctcaacaat gttgatagtg
     4921 aatttggcga gattattggc cgcagcgaag ccatgtacag cgtgcttaaa caagttgaaa
     4981 tggtggcgca aagtgacagt accgtgctga tcctcggtga aactggcacg ggtaaagagc
     5041 tgattgcccg cgcgatccat aatctcagtg ggcgtaataa tcgccgcatg gtcaaaatga
     5101 actgcgcggc gatgcctgcc ggattgctgg aaagcgatct gtttggtcat gagcgtgggg
     5161 cttttaccgg tgccagcgcc cagcgcattg gtcgttttga actggcggat aaaagctctc
     5221 tgttcctcga cgaagtgggc gatatgccac tggagttaca gccgaagttg ctgcgtgtat
     5281 tgcaggaaca ggagtttgaa cgtctcggca gcaacaaaat cattcagacg gacgtacgtt
     5341 taattgccgc gactaaccgc gatctgaaaa aaatggtcgc cgaccgagag ttccgtagcg
     5401 atctctatta ccgcctgaac gtattcccga ttcacctgcc gccactacgc gagcgtccgg
     5461 aagatattcc gctgctggcg aaagccttta ccttcaaaat tgcccgtcgt ctggggcgca
     5521 atatcgacag cattcctgcc gagacgctgc gcaccttgag caacatggag tggccgggta
     5581 acgtacgcga actggaaaac gtcattgagc gcgcggtatt gctaacacgc ggtaacgtgc
     5641 tacagctgtc attgccagat attgttttac cggaacctga aacgccgcct gccgcaacgg
     5701 ttgtcgccca ggagggcgaa gatgaatatc agttgattgt gcgcgtgctg aaagaaacca
     5761 acggcgtggt tgccgggcct aaaggcgctg cgcaacgtct ggggctgaaa cgcacgaccc
     5821 tgctgtcacg gatgaagcgg ctgggaattg ataaatcggc attgatttaa ctgcaaattg
     5881 ccggacagat ctgcctgtcc ggcatactat tcacgaggtt ttttcggacg atatttttcc
     5941 ggcagttctg gcaccggacg cttgtcatcg atgagatgac gcacggttaa gatcggatga
     6001 cgccacagca ttctcggccc ggcccaacgc ataatctgtt tcatctcttc acgctttgca
     6061 ggctggtaac agtgcaccgg acactgctta caggctggtt tctcttcgcc gaacacacat
     6121 ttatccagcc gcttttgcgc gtaaacaaac aacgcctcgt aatgctccgg ctccgctgac
     6181 gcctgcgggc atttcgcttg ataaagatcg atcatttttt taatcgtcag tttttcacga
     6241 gagatacgct tgccggacat gctgcctcca tactcttaag gtgtatttat attacaactt
     6301 aatttgaaag gggactatgg ctctgaagga ggaggtttat tcaaccgacg caattttgcg
     6361 tacttgcttc ataagcatca cgcaaaagct gcaaaacagc atctttcccg gaaccagcat
     6421 caagaactcg cagttcgctt cttcccctga aatgattaac tccggtatca tgtgcgcctt
     6481 atgtgattac aacgaaaata aaaaccatca caccccattt aatatcaggg aaccggacat
     6541 aaccccatga gtgcaataga aaatttcgac gcccatacgc ccatgatgca gcagtatctc
     6601 aggctgaaag cccagcatcc cgagatcctg ctgttttacc ggatgggtga tttttatgaa
     6661 ctgttttatg acgacgcaaa acgcgcgtcg caactgctgg atatttcact gaccaaacgc
     6721 ggtgcttcgg cgggagagcc gatcccgatg gcggggattc cctaccatgc ggtggaaaac
     6781 tatctcgcca aactggtgaa tcagggagag tccgttgcca tctgcgaaca aattggcgat
     6841 ccggcgacca gcaaaggtcc ggttgagcgc aaagttgtgc gtatcgttac gccaggcacc
     6901 atcagcgatg aagccctgtt acaggagcgt caggacaacc tgctggcggc tatctggcag
     6961 gacagcaaag gtttcggcta cgcgacgctg gatatcagct ccgggcgttt tcgcctgagc
     7021 gaaccggccg accgcgaaac gatggcggca gagctgcaac gcactaatcc ggcggaactg
     7081 ctgtatgcag aagatttcgc cgagatgtcg ctgattgaag gccgtcgcgg cctgcgccgt
     7141 cgcccgctgt gggagtttga aatcgacacc gcgcgccagc agttgaatct gcaatttggc
     7201 acccgcgatc tggtcggttt tggcgtcgag aacgcgccgc gcggactttg tgctgccggt
     7261 tgtctgttgc agtatgcgaa agatacccaa cgcacgaccc tgccgcatat tcgttctatc
     7321 accatggaac gtgagcagga cagcatcatt atggatgccg cgacacgtcg taacctggaa
     7381 attactcaga acctggcggg cggtgcggaa aatacgctgg cttctgtgct cgactgcacc
     7441 gtcacgccga tgggtagtcg tatgctgaaa cgctggctgc atatgccagt gcgcgatacc
     7501 cgcgtgttgc ttgagcgcca gcaaactatt ggcgcattgc aggatttcac cgccgagttg
     7561 cagccggtac tgcgtcaggt cggcgacctg gaacgtattc tggcacgtct ggctttacga
     7621 actgctcgcc cacgcgatct ggcccgtatg cgccacgctt tccagcaact gccggagctg
     7681 cgtgcgcagt tagaaactgt cgatagtgca ccggtacagg cgctacgtga gaagatgggc
     7741 gagtttgccg agctgcgcga tctgctggag cgagcaatca tcgacacacc gccagtgctg
     7801 gtacgcgacg gtggtgttat cgcatcgggc tataacgaag agctggatga gtggcgcgcg
     7861 ctggctgacg gcgcgaccga ttatctggag cgtctggaag tccgcgagcg tgaacgtacc
     7921 ggcctggaca cgctgaaagt tggctttaat gcggtgcacg gctactacat tcaaatcagc
     7981 cgtgggcaaa gccatctggc acccatcaac tacatgcgtc gccagacgct gaaaaacgcc
     8041 gagcgctaca tcattccaga gctaaaagag tacgaagata aagttctcac ctcaaaaggc
     8101 aaagcactgg cactggaaaa acagctttat gaagagctgt tcgacctgct gttgccgcat
     8161 ctggaagcgt tgcaacagag cgcgagcgcg ctggcggaac tcgacgtgct ggttaacctg
     8221 gcggaacggg cctataccct gaactacacc tgcccgacct tcattgataa accgggcatt
     8281 cgcattaccg aaggtcgcca tccggtagtt gaacaagtac tgaatgagcc atttatcgct
     8341 aacccgctga atctgtcgcc gcagcgccgt atgttgatta ttaccggccc gaacatgggc
     8401 ggtaaaagta cctatatgcg ccagaccgca ctgattgcgc tgatggccta catcggcagc
     8461 tatgtaccgg cacaaaaagt cgagattgga cctatcgatc gcatctttac ccgcgtaggc
     8521 gcggcagatg acctggcttc cgggcgctca acctttatgg tggagatgac tgaaaccgcc
     8581 aatattttac ataacgccac cgaatacagt ctggtgttaa tggatgagat cgggcgtgga
     8641 acgtccacct acgatggtct gtcgctggcg tgggcgtgcg cagaaaatct ggcgaataag
     8701 attaaggcat tgacgctgtt tgccacccac tatttcgagc tgacccagct accggagaaa
     8761 atggaaggcg tcgccaacgt gcatctcgat gcgctggagc acggagacac cattgccttt
     8821 atgcatagcg tgcaggatgg cgcggcgagc aaaagctacg gcctggcggt tgcagctctg
     8881 gcaggcgtgc caaaagaggt gattaagcgc gcacggcaaa aactgcgtga gctggaaagc
     8941 atttcgccga acgccgccgc tacgcaagtg gatggtacgc aaatgtcttt gctgtcagtg
     9001 ccagaagaaa cttcgcctgc ggtcgaagca ctggaaaatc tcgacccgga ttcactgact
     9061 ccgcgtcagg cgctggagtg gatttatcgc ttgaagagtc tggtgtaata acaattcccg
     9121 atagtctttt gctatcggga atattaacga caactgacga atcaaataaa aataccctgt
     9181 ataataggaa agcttatttt acagggtaaa accatgccat ctacacgcta tcaaaaaatc
     9241 aatacccatc actatcgcca tatatgggtc gttggtgata ttcatggtga atatcagtta
     9301 ttacaatccc gcttacatca actctctttt ttccccgaaa ccgacttact tatttctgtc
     9361 ggcgataata ttgatcgtgg gccggagagt cttgacgtcc tgcgcctgct aaaccagccc
     9421 tggtttatct ccgttaaagg caaccacgaa gcaatggcgc tggatgcgtt cgcgaccggc
     9481 gatggcaata tgtggcttgc cagcggtggt gactggtttt tcgatttaaa tgattcagag
     9541 caaaaagaag ctacagatct gttgctgaaa ttccatcacc ttccacatat tattgaaatc
     9601 actaacgaca acataaaata tgtcatcgca catgcagatt atccggggag tgaatatctc
     9661 tttggtaaag aaatagcgga gagcgaatta ctctggcctg ttgatcgtgt gcagaaatcg
     9721 cttaatggcg agttacaaca aataaacggc gctgattatt ttatatttgg acatatgatg
     9781 tttgataaca ttcagacgtt cgctaaccag atttatattg ataccggcac gccgaaaagc
     9841 gggcggctgt cattttataa aataaagtag tctcatgctt cttctgtgaa gcatgagtaa
     9901 ccaaatgtta ttgcaggcca ttattcattt ttcgctacca gcaaagagag atcctgcttc
     9961 gccagcgcgc gactggcact ctccggcaaa ccgtcgtctg taataatctg atcaaactcg
    10021 cttaatggta acgccagcca tgtcgccacc tgaccatatt tcgtcgcatc acagaccaaa
    10081 actcgctggc ggctggcact ggcaatcgcc cgtttcaccg tgactttatc ttccgctggc
    10141 gtagaaatcc cccgcacact ccatgacgat gcagaaataa aagcctgatc aatcatcagg
    10201 ctgcgcagca tggtcgcagc ggcttccccg acacaggaac ggttttcccg acacactgca
    10261 ccgccagtgt gaataattgt gcaattactg ttgtcgagca agtagttcgc aataacgaaa
    10321 tcgtttgtga ccacagtcag tgactccatg tgaatcagat gctgtgctat cgctaacgtg
    10381 gtcgttcccg catccagata gatacaactt cccggctgaa caagacttgc cgccagcttg
    10441 ccaatagccg ctttttgcgt cattgccagc gcagttttta cctgatgaga aggttcatgc
    10501 gccacgcgtc ccggagactg gacgcctccg gacaccagca caacggctcc ctgctgctcc
    10561 agtttttgta aatcccgacg aatggtcata tgtgacacat tcattctgtc cgttagttca
    10621 gcaatactga caatgccttt ttcagctacc atctcaagga tgatttggcg acgctctacg
    10681 ggtatcaact tttgctcctt cctttgtcct gctgacattc tacgctattt gcctgcgaaa
    10741 cgtgcgcggc gcaactaacg cttagttcac ataaaataac acacaatgtt aatttatgtg
    10801 aatcagctca ccatatcgtt atcttccagc gcttatattc acaatatcaa acaaaatatc
    10861 acttaaatta acaaggagag cagatgaaaa cgggatctga gtttcatgtc ggtatcgttg
    10921 gcttagggtc aatgggaatg ggagcagcac tgtcatgtgt ccgcgcaggt ctttctacct
    10981 ggggcgcaga cctgaacagc aatgcctgcg ctacgttgaa agaggcaggt gcttgcgggg
    11041 tttctgataa cgccgcgacg tttgccgaaa aactggacgc tctgctggtg ctggtggtca
    11101 atgcggccca ggttaaacag gtgctgtttg gtgaaacagg cgttgcacaa catctgaaac
    11161 ccggtacggc agtaatggtt tcttccacta tcgctagtgc tgatgcgcaa gaaattgcta
    11221 ccgctctggc tggattcgat ctggaaatgc tggatgcgcc agtttctggt ggtgcagtaa
    11281 aagccgctaa cggtgaaatg actgtcatgg cctccggtag cgatattgcc tttgaacgac
    11341 tggcacccgt gctggaagcc gttgccggaa aagtttatcg cataggtgca gaaccgggac
    11401 taggttcgac cgtaaaaatt attcaccagt tgttagcggg cgtacatatt gctgccggag
    11461 ccgaagcgat ggcacttgca gcccgtgcgg ggatcccgct ggatgtgatg tatgacgtcg
    11521 tgaccaatgc cgccggaaat tcctggatgt tcgaaaaccg gatgcgtcat gtggtggatg
    11581 gcgattacac cccgcattca gccgtcgata tttttgttaa ggatcttggt ctggttgccg
    11641 atacagccaa agccctgcac ttcccgctgc cattggcctc aacagcattg aatatgttca
    11701 ccagcgccag taacgcaggt tacgggaaag aagacgatag cgcagttatc aagattttct
    11761 ctggcatcac tctaccggga gcgaaatcat gatcaagatt ggcgttatcg ccgatgattt
    11821 taccggcgcg acggatatcg ccagttttct ggtggaaaac ggtctaccaa cggtacaaat
    11881 taacggtgtt ccaacaggta aaatgccgga agcaatcgac gcactggtga tcagcctgaa
    11941 aacgcgctcc tgtccggtgg ttgaagccac acagcaatcg ctggcggctc tgagctggtt
    12001 gcaacagcaa ggttgcaaac agatctattt caaatactgc tctactttcg acagtacggc
    12061 gaaaggtaat atcggcccgg ttaccgatgc attaatggat gctctcgaca cgccgtttac
    12121 ggtcttctct ccggccctgc cggtcaacgg acgtacggtt tatcaggggt atttgttcgt
    12181 aatgaatcaa ctgctggccg aatccgggat gcgccatcat ccggtaaatc ccatgaccga
    12241 cagctatctt ccccgtctgg ttgaagcgca atccacaggg cgctgcggcg tcgtttcggc
    12301 acatgttttc gaacaaggtg tggatgccgt tcgtcaagag ctggctcgct tacagcaaga
    12361 gggctaccgc tacgcggtgc ttgatgcgct gaccgaacac catctggaaa ttcagggaga
    12421 agccttgcgc gatgccccac tggtaacggg cggttctggt ctggcgattg gcctggcccg
    12481 gcagtgggcg caagaaaacg gtaaccaggc tcgcgaagca gggcatccgc tcgctgggcg
    12541 cggcgtagtg ctctccggtt catgctctca aatgaccaac cgccaggtgg cacattaccg
    12601 tcaaattgca ccagcccgtg aagttgatgt ggcacgctgc ctctcaaccg aaactctggc
    12661 cgcttatgca cacgaactgg cagagtgggt tctgggccag gaaagtgtac ttgctccact
    12721 ggtttttgcc accgccagca ctgacgcatt ggcagcaatt caacagcaat acggtgcaca
    12781 aaaagccagt caggcagtag aaactctatt ttctcaacta gcggcgcggt tagcagcgga
    12841 aggcgtgaca cgctttattg tcgcaggcgg tgagacctcc ggcgtagtca cacagagcct
    12901 gggcataaaa gggtttcata ttggcccaac catttccccc ggcgtgccgt gggtaaacgc
    12961 actggataag cctgtctcac tcgcccttaa atctggcaac ttcggtgatg aagccttttt
    13021 ttcacgagcc caaagagagt ttttatcatg agcgatttcg caaaagtaga gcagtctttg
    13081 cgagaggaga tgacgcggat tgccagttca ttctttcagc gcggctatgc aaccggttcg
    13141 gctggcaatc tgtcgctgct tttacctgac gggaatttac tggcgacacc gacaggttca
    13201 tgcctgggca atctcgatcc gcagcggctt tccaaagtcg ccgcggatgg cgaatggtta
    13261 agtggtgaca aaccctcgaa agaggtgctc tttcatctgg cgctgtatcg caacaatccg
    13321 cgctgtaaag cggtggtgca tttgcacagc acatggtcga cggcgctttc ctgcctgcaa
    13381 gggctggaca gcagcaacgt tattcgtccg ttcacaccat acgtggtgat gcggatggga
    13441 aatgtcccgc tggtgcctta ttaccgaccg ggcgataaac gcatcgcaca ggatctggcg
    13501 gaactggcag cagacaatca ggctttttta ctggcaaatc atggcccagt ggtttgcggt
    13561 gaaagcctgc aagaagccgc caacaatatg gaagagctgg aggaaacggc aaagctgatt
    13621 tttattctcg gtgaccgccc gatccgttat ctgaccgcag gtgaaattgc ggaattaagg
    13681 agttaatgca atgcctcgtt ttgcagctaa tttatccatg atgttcaccg aagtgccttt
    13741 tattgaacgc ttcgccgcag cgcgaaaagc cgggttcgat gctgtggaat ttctgtttcc
    13801 ctatgactac tccaccctgc aaatccaaaa gcaactggag caaaaccatc tgacactggc
    13861 gctgttcaat accgctcccg gagatattaa tgcgggggaa tggggattat ccgcccttcc
    13921 cggacgtgag cacgaagcac acgcagacat cgacctggca ctggaatatg cattggcgct
    13981 caattgtgaa caagtccatg tgatggcagg cgtcgtgccc gctggcgaag acgctgaacg
    14041 gtaccgggca gtatttatcg ataatctccg ctacgctgcc gaccgctttg cgctacacgg
    14101 caagcgaata ttagttgaag cactcagccc cgacgttaag cctcattatc tcttctccag
    14161 ccagtatcag gcactggcta ttgttgaaga ggttgcgcga gataacgtgt ttattcaact
    14221 agatactttt catgcgcaaa aagtagatgg caacctgacg catttaatcc gcgactacgc
    14281 tgggaaatat gcgcacgtac aaattgccgg actacctgat cgacatgaac cggacgatgg
    14341 agaaatcaac tatccgtggc tgttccgcct gttcgatgag gtgggatatc agggctggat
    14401 cggttgtgaa tataaacctc gtggcctcac cgaagaaggg cttggctggt ttgacgcctg
    14461 gcgctaattc gttaaatccc taattacaac gtacccatac atccccccat ctacggatgg
    14521 ggtaggttaa ctataacttt cagacagggt ttcccatgtc cacaattaca ttgttatgca
    14581 ttgcgttagc tggcgtaatc atgctgttgc tgctggtcat caaggcaaag gtacaaccat
    14641 tcgttgctct gctcctcgtc agcctgttag tcgcacttgc ggcgggtata ccggcgggcg
    14701 aagtgggtaa agtgatgatc gccgggatgg gcggtgtgct tggttccgtc actattatta
    14761 ttggtctggg cgctatgctg gggcgtatga tcgaacactc tggtggtgca gagtcactgg
    14821 ctaattattt cagtcgcaag ttaggtgaca aacgaactat cgctgcgctg actctggcag
    14881 cgttcttcct cggtattccc gtcttctttg atgtcggctt tattattctt gcgccaatca
    14941 tttacggttt tgccaaggtt gccaaaatat cgccactcaa atttggcctg cctgtcgctg
    15001 gaatcatgct aactgttcat gtggcggtac cgccgcatcc aggccctgtc gccgcagcgg
    15061 ggttactcca cgcagacatc ggctggctaa ccatcatcgg tattgcgatt tctattcccg
    15121 tagggattgt tggctacttt gcagcgaaaa taatcaatac gcgccaatat gcgatgtcgg
    15181 tggaagtact ggaacagatg caactggctc cggccagtga ggaaggcgca acaaaattaa
    15241 gcgataaaat aaatccaccg ggcgtcgcgc tggtcacctc gctaattgtt attcctatcg
    15301 cgattatcat ggcgggtacg gtttccgcaa cactgatgcc gccttcgcat cccctgcttg
    15361 gtacgctaca gctgatcggc tcaccaatgg tagctctaat gattgcgctg gtgctggcat
    15421 tctggttatt ggctttacgt cgcggctgga gcttacaaca caccagcgac attatgggct
    15481 cagcccttcc cactgcggcg gtagtgattt tggttactgg tgctggaggg gtatttggca
    15541 aagtgctggt ggaatcgggc gtcggcaaag cccttgccaa tatgctacaa atgattgacc
    15601 tgcctctgtt accagccgca tttattattt cactggctct gcgtgcatcg caggggtcag
    15661 caaccgtagc aatcctgaca accggcgggt tactctcaga agcggtgatg ggattgaatc
    15721 cgattcagtg cgtattggtg acgctggcag cctgctttgg tgggcttggc gcgtcacata
    15781 ttaatgactc agggttctgg attgtgacca aatatctggg gttgtcggta gcagacggtc
    15841 tgaaaacctg gacagtgtta acgaccattc tcggttttac cggattctta attacctggt
    15901 gcgtatgggc ggtaatttga ccttaataaa aaggtccgat gggcatcgga ccttttattt
    15961 aacgcttacc ttccaccagc agcggcggga tgctcggcac cattggcgcg tcatcaatat
    16021 ctttctgcgt catgcggaac gcttcgggat aatgttcgcg gctggtacgg cgcagcgatt
    16081 cggtatcgcg ccaggtataa aggcaatgct ggcactgata taccgtccag acatctttca
    16141 ccggcgattt cgccatcact tcaatctgtt catcggcaca acgtggacaa atcatcttct
    16201 tctccttatt tacgtgcagc cagcatagcg gtcagttttt cagcccaggc tttggtttcc
    16261 ggtaaatcca ccaccggttg gctgtagtga ccacggttgt ccggggcgac aggcgtagtg
    16321 gcgtcgataa tcagcttgtc ggtgatcccc gcagggcttg agcctggatc gagttccagc
    16381 acggacatat tcggcaactg caccaaatcc cctgccgggt tcactttcga ggagagcgcc
    16441 cacatcacct gcggcaggtt gaacgggtca acgtcttcat cgaccataat caccatcttc
    16501 acgtagccca gaccatgcgg cgtggtcatt gcgcgcaggc ccaccgcgcg ggcaaagccg
    16561 ccgtagcgtt ttttggtgga gataatcgcc agcaggccat gggtgtacat ggcgtttacc
    16621 gcctgcactt cagggaactc ggctttcagc tgctgataca gcggcacgca ggtggctggc
    16681 cccatcaggt agtcgatttc ggtccacggc ataccgaggt acagcgattc gaaaatcggc
    16741 ctggtgcggt aagagacttt atcgatgcgc accacggtca tgttacgccc gccggagtag
    16801 tgcccggtaa actcaccgaa cggcccttcg atttcgcgtt tacggctttc gatgacccct
    16861 tcgaggatca cttctgaacc ccacggcaca tcaaaaccgg tcaacggggc ggtggcgatc
    16921 gggtacgggc tttcacgcag cgcgcctgcc atttcgtact cggactgatc atatttcagc
    16981 ggcgtggccc ccatcagcgt gatgatcgga tcgttaccga gcgtgatcgc aatcggcaga
    17041 tcttcaccgc gctcttctgc tttatgcaga tgcagggcga tatcgtgcat cggcaccggt
    17101 tgcaggccga gcttacgctt gcccttcact tccatgcggt agatgccgac gttctgcttg
    17161 ccgaagttat ccgggtcgag cggatcgcgg gaaaccacgc acgctttgtc gagatagaaa
    17221 ccgccatcgc catcgtttaa acgaaacagc ggcaggatat cgaacaggtt gatctcgtcg
    17281 ccatcaacgg tgttctgcgc ccaggctgga ttggcgcggc gctccggggc aatcgggaag
    17341 ttatcccagc ggcggataaa ctcatcaatc tgctttttaa ccggggcatt tggcggcagg
    17401 ccgagggaaa tcgcgtggtt ctgccaggaa ccgatggtgt tcatcgccac gcgggcatcg
    17461 gtaaagccgc gaatattatc aaaccacagc gcgggcgcgc cgtcgccgat acgcccggtg
    17521 gcgttagctg ctgctgccag atccggctcg gcgttcactt cttcgctgat tttcagtaac
    17581 tggccgtggt catcaagcgc ctgtaaaaag ctgcgtaaat catcaaatgc cattattcat
    17641 tctcctgaga aaaattccgg gcctgcggca atccttgcca gcgcctggcg tggggatgtt
    17701 cgaggccaaa ttgatccagc acgcgggcta ccacatggtg gacaatgtca tctaccgttt
    17761 cgggatggtt ataaaaggca ggcatcggcg gcaccatcgc cacgcccatg cgtgaaagtg
    17821 cgagcatatt ttcgagatgg atggtgctaa gcggcatttc acgcggcacc agcaccagtt
    17881 tgcggccttc tttgagcacg acgtccgccg cgcgccctac caggccatcg gcgtaaccag
    17941 cgcggatacc ggcgagcgtt ttcatactgc acggaataac gatcatgccg tcggtacgaa
    18001 aagaaccgga tgagatgatc gccgcctgat ccgccgggtt atggctgaag tctgcgaggg
    18061 cagcaacatc gcgagcgctg taaggcgttt ccagttcaat ggtggttttc gcccacttcg
    18121 acatcaccag atgagtctcg acattcggca tctcccgcag cgcttgcagt aatgccacac
    18181 caagaggcgc accggtagcc cctgtcatcc cgacgatcag tttcattgct tatcctctta
    18241 attgttcgta tacgaacgtt attattaaca tactccttca tttccacagc ggcaaggatt
    18301 gaaaagtaac aacatcttct tcgtcactaa aaaaccgcta tgatagcgac agagaattag
    18361 cccggagttt acatggcgtt acgaaataaa gcgttccatc agttacgaca gctttttcag
    18421 cagcacacgg ctcgctggca gcacgagtta cctgacctga ccaaaccaca gtacgcggtg
    18481 atgcgcgcca ttgctgataa acctggtatc gaacaggtgg cactgataga agcagctgtc
    18541 agcaccaaag caacgttagc agaaatgttg gcaagaatgg aaaatcgtgg cttagtcaga
    18601 cgtgaacatg accccgctga taagcggcga cgctttgtct ggttgactgc cgaaggcgaa
    18661 aagatacttg ctgcggcgat acccattggt gacagcgtgg atgaggaatt tttggggcgt
    18721 ttgagtggag aagagcagga gctgtttatg cagctggtgc gcaagatgat gaacgcatag
    18781 ggggcaaatt tccggatgcg acgctatcgc gtcttatcca gactacaaat acaccgactc
    18841 accaaccgaa agaaaaaagg ccagcctcgc ttgagactgg cctttctgac agatgcttac
    18901 ttactcgcgg aacagcgctt cgatattcag cccctgcgtt tgcaggattt cgcgcaaacg
    18961 gcgcaggcct tcaacctgaa tctggcgaac acgttcacgg gtgaggccaa tttcacgacc
    19021 tacatcttcc agtgttgccg cttcgtaccc cagcaaaccg aatcgacgtg ccagcacttc
    19081 acgctgtttg gcgttcagct cgaacagcca tttgacgatg ctctgcttca tatcgtcatc
    19141 ttgcgtggta tcttccggac cgttctcttt ttcatcggcc aggatgtcca gcaacgcttt
    19201 ttcggaatca ccacccagcg gggtgtctac cgaggtaatg cgctcgttaa gacgaagcat
    19261 acggctgacg tcatcaactg gcttatccag ttgctctgcg atctcttccg cacttggttc
    19321 atggtccagc ttatgggaca actcacgtgc ggttcgcagg taaacgttca gctcctttac
    19381 gatgtgaatc ggcaaacgaa tagtacgggt ttggttcata atcgcccgtt caatcgtctg
    19441 gcgaatccac caggttgcgt atgttgagaa gcggaaacca cgttccgggt caaacttctc
    19501 taccgcgcgg atcagcccca ggttgccctc ttcgataagg tccagcaacg ccagaccacg
    19561 attgccataa cggcgggcaa tttttaccac cagacgcaag ttactctcga tcatccggcg
    19621 gcgagaggcg acatctccac gcagtgcgcg acgcgcaaaa taaacttctt cttcggccgt
    19681 taacagtggt gaataaccaa tctcaccaag gtaaagctga gtcgcgtcca acacacgctg
    19741 tgtggctccc tgcgataaca gttcctcttc ggccaaatcg ttatcactgg gttcctcttc
    19801 tactaaggcc ttttcgtcaa aaacctcaac tccgttctca tcaaattccg catcttcatt
    19861 taaatcatga actttcagcg tattctgact cataaggtgg ctcctacccg tgatcccttg
    19921 acggaacatt caagcaaaag cctggttccg ccgatttatc gctgcggcaa ataacgcagc
    19981 gggtttacgg atttcccctt gtaacgaatt tcaaaatgca agcgtgttga actggttccg
    20041 gtgctaccca tggttgctat tttttgcccc gccttcactt cttgttgttc ccggaccagc
    20101 attgtgtcgt tatgggcgta ggcactcagg taatcatcat tatgtttgat gataatcaga
    20161 ttaccgtagc cgcgcagcgc gttaccggca taaacaacgc ggccatctgc ggtcgcgata
    20221 attgcctgtc ctttgctgcc tgcgatatca atccccttgt tgcccccctc agaagcgcca
    20281 aaggtttcga tcactttgcc ctcagtcggc cagcgccagg tggagatagg cgtactggtt
    20341 gatgtactgc tgacagttgg ctcggttgtg cttgctgttg gtaccgttac aggcgctgtg
    20401 accgtggtcg cagctggctt gttgttcggc aacattttgt tagcactctg ttcacccgaa
    20461 gattcagaat acgtaattgt cggttgcgac gcaacagcaa cggtggaatt ttgtgcaggc
    20521 ttgatcacaa ctccttgctc tgctgcgtcg gcctgggtaa tggcatttcc gccagtgatt
    20581 ggcgtaccgg aagcattacc cacctgcaag gtctgaccaa cgttcagcgc gtatggtgcc
    20641 tgaatattgt tgcgctgagc aaggtcacgg aaatcgttgc cagtaatcca ggcgatatag
    20701 aaaagtgtgt cgcctttttt cacggtatag gtactgccgc tataactgcc tttcggaatg
    20761 ttcccatact gacggttata gacgatgcgt ccgttttcca tctgtaccgg ctgctgagct
    20821 actggctgca ctggctggat ttgcggttgt tgagtagcct gaatttgtgg ctgctgtacc
    20881 ggctgaattt gcggttgctg ggctgtagac gtcgtcccca ttttcggcgg cggcgtaatc
    20941 aacataccag aattggtatt tgcaggcgca ttgccattaa cggagctgac cggtgccggt
    21001 ggatttgaag tgtcagaaca gcctgccagc catagcgaaa ccagtgacaa agccgcaatg
    21061 cggcgaacgg tgaattttgg gcttcccgcg ctcatttatc ccccaggaaa aattggttaa
    21121 taaccagtga cataattacc gtgcaaggca ccctactgaa cactggaaaa gatgttcacg
    21181 atacgctgac ctgcggcaaa ataaccagga aaaatccagg tatttcctca cgttttaagc
    21241 cagctcaccc ttcactaaag gtacaaagcg cacggcctcc acggtatcga taataaattc
    21301 gcctccccga cgacgcaccc gtttcaaata ctggtgctcc tcccctacgg gtaagacgag
    21361 aatcccgcct tcgtccagct gcgtcattag cgcagttgga atttccggcg gtgccgccgt
    21421 aacaatgata gcgtcaaacg gcgcgcgtgc ctgccaacct tgccatccat cgccatgacg
    21481 ggttgaaaca ttatgtaaat caagattttt caggcggcga cgcgcctgcc actgcaaacc
    21541 tttaatccgt tcgaccgagc aaacatgctg gacaagatgc gccaggattg ccgtttgata
    21601 tcccgaacca gtgccaattt ccagcacccg cgactgcggc gtcagctcga gtaattcggt
    21661 cattcgcgcc accatatacg gctgcgaaat tgtctgcccc tgacctatcg gcaaggcgat
    21721 attgtcccag gctttttgtt caaacgcttc atcaacgaat ttttcacgcg gcacggcggc
    21781 aagtgcattc agcacctgct catcctgaat accttgcgca cgtaattgat ccagaagtgc
    21841 ttgtacgcgt ctgcttacca ttgcgtgcca actcccacgc tgtttaacca gtctgaaacc
    21901 acatcttgcg cgctatgcgc agttaaatcc acatgcagcg gcgtgatgga gacatagccc
    21961 tcatctaccg cagcaaaatc ggtccccgga ccggcatcac atttaccgcc cggcgggcca
    22021 atccagtaca gcgtattgcc gcgcggatct tgctgcggga tcacctgatc tgccggatgt
    22081 cgtgtaccgc agcgcgtcac gcgaatacct ttgatttgat ccaagggtaa atccggaacg
    22141 ttaatattaa gaatacgccc ggtgcgcaac ggctctttac acagtgcgcg caaaattgaa
    22201 caggttaccg ccgcggcagt gtcgtaatgt ttatgcccgt caagcgagac ggcaagcgcc
    22261 ggaaaaccta aatgacggcc ttccatcgcg gcggctaccg taccggaata aataacatca
    22321 tcccccagat tcggcccggc gttaattccg gacacaacaa tgtccgggcg cggacgcatc
    22381 agagcattca cgccaagata gacgcaatcg gtcggggttc ccatttgcac agcaatatca
    22441 ccattttcaa aggtaaacgt gcgcagggag gattccagtg tcagagaatt tgaagcgccg
    22501 ctgcggttac gatcgggggc gaccacctga acgtcagcaa actcacgcaa ggctttcgcc
    22561 agcgtttgta taccgggtgc atgtaccccg tcatcattac tcagcaatat gcgcataatc
    22621 acctgttgtg ttgataagtt ccctgacaac gctggttgca aaactacccg ccggaagcca
    22681 gaaacggatc tctacggtga cgtcatccca ccaattccag cttaattgtt gcggatacag
    22741 cagcatcgct ctgcgcgcgg cttcaacttt ttcgcgcacc agtaaagctt gtaattcagt
    22801 ttctgcggcg acagctgctt gttcgaatgc cagcgcttca cgctgagttc cccattcgcc
    22861 actgcccggc aatgcggcgg ttatcatcag ctctttatcg ttaacgcgac gctgtaattc
    22921 cgccagttct tcggtggttg cgacaaacca gctaccacgt ccggctaatt gtagcgcatc
    22981 gccgtcaaca acttgattaa cgtctgcttt tttgaggcgc tcagcaacaa tctgattaaa
    23041 caacgcactg cgggctgccg acaaccaaaa actccgttta ttgcgatcgc gcaccggagt
    23101 attggtttgc gcccagcgct gcgccccctg caagttgcta ccgccaatcc caaaacgttg
    23161 ggcaccgaag tagttcggta caccttttac gcaaatatcg ttcagacgtt gttcaacgtc
    23221 atcgcgattg ctcacttcgc gcagaaccag ggtaaaggcg tttcctttca gcgcccctaa
    23281 acgcagcttg cgcttgtgcc gcgcatactc cagcacctgg cagccttcca gttgaaaggc
    23341 gctcagatcg ggcatttcct tgcccggcac gcgagcgcat aaccactgtt ccgtaacagc
    23401 atgtttgtct ttttgcccag cgaagctgac ttcacgggca tgaattttca ggaatttcgc
    23461 cagtgcatcc gccacaaaac gggtattgca gccgtttttg aggattctaa ccagaatatg
    23521 ctcaccttca ccatcaggct caaagcccaa atcttccacc accacaaagt cttccggatt
    23581 ggctttcagc agcccggtgc cttgcggttt accgtggagg taagtgagat tatcaaactc
    23641 aatcattttg ttgccttaat gagtagcgcc accgcttcac aggcaatccc ttccccacgt
    23701 ccggtaaatc ccagtttttc cgtagtagtg gctttcacgt taacatcatc catatggcag
    23761 ccgagatctt cggcaataaa cacgcgcatt tgtggaatgt gcggcaacat cttcggtgcc
    23821 tgagcgatga tagtgacatc gacgttgcca agggtataac ccttcgcctg aatacgacgc
    23881 caggcttcgc gtagcagctc gcggctatcg gcacctttaa atgccggatc ggtatccggg
    23941 aacagcttgc cgatatcccc cagcgccgcc gcgccaagca atgcatcggt caacgcatgg
    24001 agcgccacgt cgccatcaga atgcgccagc aatccttttt cgtaaggaat gcgtacgcca
    24061 ccaatgataa ttgggccttc accgccaaag gcatgtacgt caaaaccgtg tccaattcgc
    24121 attatgtatt ctcctgatgg atggttcggg tgaggtaaaa ctcggccagt gccaaatctt
    24181 ccgggcgcgt gactttaatg ttatccgcac ggccttcgac caactgagga tggaatccgc
    24241 aatattccag cgccgaggct tcgtcggtaa tagtcgcgcc ttcatttaga gcgcgcgtca
    24301 gacagtcatg taacagctca cgagggaaaa attgcggcgt cagcgcgtgc cataagccgt
    24361 tgcgatcaac ggtatgagca atggcatttt tgcccggttc ggcacgtttc atagtatcgc
    24421 gcactggtgc ggcgaggatc ccccccgtgc ggctggtttc gctcaacgcc aacaatcgcg
    24481 cgaggtcatc ctgatgcaaa caaggacgag cggcgtcatg caccaatacc cactgcgcgt
    24541 cgccagcggc tttcagacct gccagcacgg aatcggcacg ctcatcaccg ccatctacaa
    24601 cggtgatttg cggatgattc gccagaggaa gttgtgcaaa acggctatcg ccaggactta
    24661 tggcaatgac gactcgtttc acccggggat gcgccagcag cgcatgcacc gagtgttcaa
    24721 gaatggtttg attaccgatt gagagatatt gcttaggaca ttccgtttgc attcgacggc
    24781 caaatccggc cgccggaacc acggcgcaaa catccaaatg agtggttgcc atgttaattc
    24841 ccgggctgat ttatcgattg ttttgccccg cagactgtgc gcgcttcgac gcgtcaggca
    24901 ccagacgata aaaagtttcg cccggcctgg tcatgctgag ttcattacgc gcacgctctt
    24961 cgagcgcctc ctggccgcca ttgagatcgt caatttcggc aaaaagttga tcgtttcgcg
    25021 ctttaagttt cgcgtttgta gcttgctgtg ccgccacatc atcattgacg cgggtatagt
    25081 catgtatacc gttcttaccg aaccacagcg aatactgtag ccagaccaga atagccagca
    25141 acagcagcgt tagtttaccc atcctgcccc ctgaaaaacg gcatcatcat cccatgcatc
    25201 cgaagacgac tctacatcct ctgttgggga taccgcgaca acgcgggcaa atgtaccaca
    25261 tttgtccatt gttacgtata cccagggcgt gcagaacata atctcattat tagttacggt
    25321 ttgaattatg aacagaggag acgggaaagt acaaattagc ccagtagcca cataaacagt
    25381 gcgccaaaca taatgcctac tgtcaccagg gtgaaaacaa tactgtagcg tagctttccg
    25441 tccatcaatg aatgcagcgc aatccccacc actaccgcga cgggcatcag cgccagaaag
    25501 aaaggccagg tgtagataaa gaagaacagc gtgttagagc cataaatcaa catcggcatc
    25561 gccagcgcaa ataaccagga gataaaaccg accacggcac caggcagtga ccatgtggtt
    25621 tcttcatcct cagtaaggct gtcgttattt gttagtgtaa tgttatggct attacgcata
    25681 tttgatcctg ttactttgac gaaccgggca tggaaacccg gtggtgtctc aggatctgat
    25741 aatatcgttc tgtctcaaca gatctaataa ttgctgtacc aaatttgtta ctaattgttc
    25801 accattgaga tgaatttctg ccgattcagg cgcttcgtaa acggaatcta ttcccgtaaa
    25861 gttgcgcagt tcaccggcac gcgctttctt atataagcct tttggatcgc gggcttcgca
    25921 aatcgccagc ggcgtatcga caaacacttc gataaagcgc ccttctccta cgcgttcgcg
    25981 aaccatctgg cgttcggcgc ggtgtggcga gataaatgcg gtcagcacta ccagtccggc
    26041 ttcaaccatc aaattcgcca cttcaccgac gcgacggata ttctctttac gatcggcatc
    26101 gctaaaaccg agatcgctgc ataatccgtg gcgaacatta tcgccatcca gcagatacgt
    26161 actgacgccg agtttatgta acgcctcctc cagcgccccg gcgaccgttg atttaccgga
    26221 cccggagagg ccggtaaacc acagcactac accacgatga ccgtggtgta gctcgcgttg
    26281 ttgcacagtg accggatggc tatgccagac gacgttttcg tcatgcagcg ccattattta
    26341 tcccccagca aatcgcgcgc gccccagtgc ggaaagtggc gacgaaccag agcattcaat
    26401 tccagttcga atgcactgaa ttcagatggc gcagcagttg cctggctaac tggctcgtgc
    26461 accataccgg cacccacggt cacattgctc aggcgatcga taaaaatcag cccacccgtc
    26521 accggatttt gttgataacg atctaacacc agcggctcgt caaaagtgag atccacgagg
    26581 ccgatcccat tcagtggcag gttttcaact tcacgctggg taaggttatt aatatcaacc
    26641 tgatagcgaa tgccatcaac acgcgcgcgc gtcttcttac cggcaatttt gatgtcgtaa
    26701 ctctgccctg gagaaagcgg ctgttccgcc atccatacca catccaccga cgcgctctgc
    26761 accgccggta acgcttcgtc tgccgccagc agcagatcgc cacggctgat gtcgatctca
    26821 tccgtcagca ccagggtgat cgcttctccg gcaaaggctt cttcgcgatc accatcaaaa
    26881 gtcacgatcc gcgcgacgtt tgattccaca ccagagggca gcactttgac acgttgcccg
    26941 acttccacgc gaccggatgc cagcgttccg gcgtaaccac gaaaatcgag attcgggcga
    27001 ttaacgtact gcaccgggaa gcgcattggc tgagcatcca ccactcgctg gatctccacg
    27061 gtttccagca cttcgagcag tgtcggaccg ctgtaccacg gcatactttc actttgcgat
    27121 gccacgttgt cgccttccag tgcagagagc ggcacaaagc ggatatccag attacccggc
    27181 agctgcccgg caaaggtcaa ataatcttca cgaatacggg tgaacgtctc ttcactgtaa
    27241 tccaccagat ccattttgtt gatcgccacg accagatgtt tgctccccaa cagtgtggag
    27301 ataaaactgt gacgacgggt ttgatcgagc acgcctttac gggcatcgat cagtaagatc
    27361 gccagttcac atgtcgatgc gccagtcgcc atattgcggg tgtactgctc gtgccctggg
    27421 gtgtcggcga taataaattt acgcttctcg gtagagaaat agcggtaggc cacgtcaatg
    27481 gtgatgccct gttcgcgctc agcttgcagg ccgtccacca gcagagccag atccagcttt
    27541 tcgccctggg tgccgtgacg cttactgtcg ttatgcagcg atgagagctg atcttcgtag
    27601 atttggcggg tatcgtgcag cagacgacca atcagagtac ttttgccgtc atcgacgcta
    27661 ccacaggtca gaaaacgcag caggctttta tgttgttgcg caatcatcca ggcttcgacg
    27721 ccgccttcat tggcgatttg ttgtgcaagt gcggtgttca tcttaaaaat acccctgacg
    27781 ttttttcagc tccatagacc ccgcctggtc gcggtcaatc acgcggccct gacgttcact
    27841 ggtggtggaa accagcatct cttcgatgat ttccggcagt gtttgtgcat ttgactccac
    27901 cgcaccggtc agcggccagc agcccagcgt acggaaacgc accatccgtt ttttaatcac
    27961 ttcgcccggt tgcaggtcga tacggttgtc atcaatcatc atcaacatac cgtcgcgttc
    28021 cagaaccgga cgttccgcag cgagatatag cggaacaatg tcgatatttt ccagccagat
    28081 gtattgccag atatcctgct cggtccagtt agagagcggg aagacgcgga tgctttcgcc
    28141 tttgttaatt tgcccgttgt agttgtgcca cagctccggg cgctgatttt tcggatccca
    28201 gcgatggaag cggtcacgga aagagtaaat tcgctcttta gcgcgggatt tctcttcgtc
    28261 acggcgcgca ccaccgaagg cggcatcaaa accgtatttg ttcagcgcct gtttcaggcc
    28321 ttcagttttc ataatatcgg tatgtttcgc gctgccgtgc acgaatggat taatccccat
    28381 cgccacgcct tccgggtttt tatgcaccag cagttcgcag ccgtaggctt tagcagtacg
    28441 atcgcggaac tcatacatct cgcggaattt ccagccggta tcgacatgca gcaacgggaa
    28501 aggcagcgta cctggataaa acgccttgcg cgccagatgc agcatgacgc tggaatcttt
    28561 accgatagag tagagcatca ccggatttga gaattctgcc gccacctcgc gaataatgtg
    28621 gatgctttcc gcctccagtt gccgcaggtg agtaagtcgt atttgatcca taaccgttcc
    28681 tttgcaatac cgctattttc ttgccatcag atgtttcgac tatagggagc gtaagagaac
    28741 gaatgaaatt accaattaga atgagtagtt ccttaacgga ataacgattt ggcaaagcta
    28801 atatcaaaaa gtgcttaagg caccggattt cgggcgttta ggaagatttg aaattgtttt
    28861 agcgcagcgg cagtttcata ctatggcggt aaaaaaattt gcatggtatt taaggactca
    28921 ctatgttttc cgcattgcgc caccgtaccg ctgccctggc gctcggcgta tgctttattc
    28981 tccccgtaca cgcctcgtca cctaaacctg gcgattttgc taatactcag gcacgacata
    29041 ttgctacttt ctttccggga cgcatgaccg gaactcctgc agaaatgtta tctgccgatt
    29101 atattcgcca acagtttcag caaatgggtt atcgcagtga tattcggaca tttaatagtc
    29161 ggtatattta taccgcccgc gataatcgca agagctggca taacgtgacg ggaagtacgg
    29221 tgattgccgc tcatgaaggc aaagcgccgc agcagatcat cattatggcg catctggata
    29281 cttacgcccc gctgagcgat gctgacgccg atgccaatct cggcgggctt acgttacaag
    29341 gaatggatga taacgccgca ggtttaggtg tcatgctgga attggcagaa cgcctgaaaa
    29401 atacgcctac cgagtatggt attcgatttg tggcgaccag cggcgaagag gaagggaaat
    29461 taggcgcaga gaatttactc aagcggatga gtgacaccga aaagaaaaat acgctgctgg
    29521 tgattaatct cgataactta attgttggcg ataaattgta tttcaacagc ggtgtaaaaa
    29581 cccctgaagc agtaaggaaa ttaacgcgcg acagggcgct ggcaattgcg cgtagtcatg
    29641 gaattgccgc aacgaccaat ccgggtttga ataaaaatta tccgaaaggc actgggtgtt
    29701 gtaatgacgc agaaatattc gacaaagcgg gcattgctgt actttcggtg gaagcgacta
    29761 actggaatct tgggaataag gatggttatc agcaacgcgc aaaaacacct gccttcccgg
    29821 cgggaaatag ctggcatgac gtaagactgg ataatcaaca acatattgat aaggcacttc
    29881 ctggaagaat agaacgtcgc tgccgtgatg ttatgcggat aatgctaccg ctggtgaagg
    29941 agctggcgaa ggcgtcttga tgggtttgaa aatgggagct gggagttcta ccgcaggggc
    30001 ggggaattct aagtgatatc catcatcgca tccagtgcgc ccggtttatc cccgctgacg
    30061 cggggaacac aaaaccaaac ttctccataa attccatagc cgcggtttat ccccgctggc
    30121 gcggggaaca ctcttcgcgg gtaatcaatg atgattcagt ttccggttta tccccgctgg
    30181 cgcggggaac acgaacgcaa tatcacggcg ttctgcggcc tcggcggttt atccccgctg
    30241 gcgcggggaa caccaaaata ttacgagctt cgtcaggcca tggaccggtt tatccccgct
    30301 ggcgcgggga acacgtcggg ccgggtaagt gaacctgtcg gttttccggt ttatccccgc
    30361 tggcgcgggg aacacactaa gcatacataa ctgtttttaa acaaatttat tccgcatcaa
    30421 caatctacca actaaattaa aacatttcct tatttttaaa gaacggataa cttattgatt
    30481 atcaacagga agaaaagaaa ccaaacgtaa cccatccaaa tccaccggaa tacgtctgtt
    30541 ttctccccag gtctgaaatt caaaacccga ctcggtattg gtcgcccagg ccatcaccac
    30601 atttccgcaa ccagccagtt gggtaatttg ctgccagatc atctcccgaa tacgttttga
    30661 tgtatcacca acatacacac cggcacgcac ttccagtagc cagattgcga gccgtccacg
    30721 taagcgcggc gggacatttt ctgtaacaac cacgaccatg ctcatcagcc gcgtcctcga
    30781 tgaccgctat cgcccaacgt ttcaggttcg gggatggctg gcggtaacat atccggcgca
    30841 ggttgtggcg gttcaatttc acctgcggca aggacttcct caattaacgg tattaatttg
    30901 cccgttaact tagtgctacg gaaaatatcg cgacaggcta atctgacttc tttatcaggt
    30961 tctgcgggtt gcctcgctgc tatttcaaat gcctttggca caaccgaatc aaatttaatg
    31021 atatcggcta tgtcataaac aaatgaaagc ggtttgccac tatggataaa cccaatagcg
    31081 ggcgcatatc ccgcggctaa tactgccgct tcagatatac cgtacagaca tgatgtagca
    31141 gcactgatgc agcgattcac aacatcgcct ttttcccagt ctttaggatc gtatttgcga
    31201 ccattccatt tcacaccata ttgtttcgcc agtaatgcat acgtctggcg aacgcgagat
    31261 ccctcaattc cacgtaactg ctccactgaa cggcgagctg gctgtggttc acgaaaacgt
    31321 aattcataca ttttgcgcac caccttcaac cgtagatctt ccgttaaagc cagctttgcc
    31381 tggtagagta atttatctgc ccgcgcccca ccgggttgtc cggaggagta aacgcgaacg
    31441 cccgcttcac cgacccagac cagcagtgtt cccaccgtgg cggccagatg caccgccgcg
    31501 tgggaaactc tcgttcctgg ttcgagcata atgcaggcga ccgatcccac cggaatatgc
    31561 gttcggatcc ctgttttgtc gatcagcacg aaagcgccgt ccagtacgtc gatctgaccg
    31621 tactggagga agatcatgga ggtgcgatct tttaatggga tcgggctcag tggtacaaac
    31681 gtcacgcctc tgctccgggt ttgatcagca tcagaccaca gccgaacgcc cggcttttac
    31741 catacccctg gctcaggcgt tgcacaaata acccaggatc ggtgaccgta agcatccccg
    31801 tataatccac gctactgaac tggatcagtt gccgggagtt ttcccgccgc agttgctgtt
    31861 gtcggtaggc atcaaccgag gtatccagca gtgtaaaacc gcttctctcc ccctgcgctg
    31921 ccagccagtc cagcgccgcc tgttgttgat gcaaccagac atcacttcct tccgccggcc
    31981 ccctcacctg ccgtttcgcc tccatcagca gatcgtggcg cttgcccgct ttacagattg
    32041 ttggattcgc ccgcaggttg aaacaaagtt gttgtccggt acacagctcg ggcgcaaatg
    32101 accggcattc tatggtgaac gtatcacttt ccgccgggcg ttcctgcgac agtacaaaaa
    32161 agcgaaacgc gccctggagt tcttcccgac gataaagaaa ttgcctttct ttgccgccag
    32221 ggaagagatc ccacagccac tgatgcatca catattcacc gcgatccacc agatgcagca
    32281 actgcgcagg cgaaagctgg ccggtatgta aggttattct tgagaggtac atggttcctc
    32341 cttgctgagc cacggcccct gattgacggt gcgttcccca aacagccact gctgacgatt
    32401 taaaggaaca tcccggcggc gtaatatttt gctcgcgacc aggccgtcgt gtttcccttc
    32461 ccaccagcat tcatcctgaa gtttcgggag tgagactttc agtttatgga aatgatcctg
    32521 atactgttgg tatgcattac ataaggcatc acacgcgttg ccttcgagca gtaacggcgc
    32581 aagaggtaat gccagcggat gactttttcg ccccagataa agcggaaaaa ctggatggcg
    32641 taaaccgtcc tgcaactgtt cgaggctgta aggcgcatcg gctgttgttg ccaccgccac
    32701 catccaccag gcatcggtgt agtagtcgcg ccgggagata atcgcgctca aaagctcagg
    32761 gtcgctcaac tcttcgcgac ggctgaaata acgtgcttta cgcacctctt ttggcatctg
    32821 gaccgtgtga taatcccgtg cccagcgcgg gttacggctg gcgcaaacca ccagtgaata
    32881 gtggcgatta aacgcgttta agcgttcggt atcatcacgc cgaatcccta ccccggcagc
    32941 cagtagcccc agcaatgctg agcgcgaagg aagttcatgg gtatgacgaa cttcacctgg
    33001 agcatcgacc ccccaggacg ccatcggccc atgaagctga aaaatcagat attggctcat
    33061 aagccgcccc ttacgcgcag ataaagtcca gcacgtcctt catgcttccc tgtttgttca
    33121 tcacgtcaaa acttgcgcat tcggtcttct gttcgtagac cgtattcatg ttttcgcgaa
    33181 gcgttgtaat acgctgcacc gccacatcta actgacgtgt gccattaatg ggttcataga
    33241 aagccgccgc cagagagcgt ggttgatcgg tgcctttttc tgccagcgcc caggaggcgt
    33301 aggcacggct ggcaaagctg ttctgtttgc cggttgggga gactttaagt gcggcttccg
    33361 taaaggcacg tatggtctga ttagctaacg cttcgtcacc gccaaggttt tcgaccagca
    33421 gatctttatc aatgcagata taggtgtaga acagtgcaga accgaatccg gtttcaccaa
    33481 gatgcccggc accggcatct tcagaagcct ggcgcaaatc atcaacggcg gtgaaaaaat
    33541 catcttcgac aatcgtttca ctgacaccaa atgcgtgcgc tacctggcag gcggcttcaa
    33601 cattaaactc gggtttattc gccagcatac gaccaaacat ggcaatatct accgccatac
    33661 ggtctttgcg taacaacgcg agatcttcct cttttggcgc gcgcttttct tctgcaagct
    33721 gatgggccag cgcttttacg gcgtcaaatt ctgccgggct gatatggact aactgttcag
    33781 tttcggcgtt agtgagcgga tcttttggtt ttttgtcatt tttagctttc ccaagataat
    33841 cagcaatttt tgccgaccat tcgatggctt ttttatcttc aatccctttc tcaatcagga
    33901 tagttgccgc ctcacgcgca atacgcccac tgcgaatacc aatatggccc gccagtgcct
    33961 gttcaaaaag tgcagaagtg cgccacgcac gtttcagact ttgcgaggaa acgcgcagac
    34021 gcgttgctcc acccagcacc acagtttttg gcgcaccggt atcatcacgg ttaaggttgg
    34081 cagcagggta agcggttaac aaatgaagct gaataaatgt tgtcatgaga tagtccttta
    34141 ttgattttgt tcgttatcgg cgtcgccagc ctggtaatat tccagcgccc agcggatacg
    34201 gataaattcg gttggccgct gctggcggcg gtgatgattg agcaggtcgt cgctctcctg
    34261 gcaccaacgg aagaccccct ctgccagaga gtcaagattg acggaaccgt ttaataatct
    34321 gactgcgcgg cgtaactggc gcagtaattc atccggtgtt tttactgccg acagacgagt
    34381 aaaacgcccc tttgacatta ccgccgccag ctgcgcggca aaaggctgcc gttcatcaat
    34441 agcttttaca ttggcgctaa ctgcggcgac cagcgccagt gcggtaaaac gccactcttg
    34501 tgcttcaatt ttccacaaag tgtgggtctg catcagcaga gaacgaaacc cttctgacaa
    34561 gcaaacatca ttaaccgttg tgctacgccg cagacttgca cgattgccgc gtttagcctg
    34621 caactcttcg cgccatttac gcagcgtggc tttgtgctcc tcttttacaa tactcattca
    34681 gcagcctcct gctttttctc cctggcggct ttagcacttt gcttctccgc cgatgttgtg
    34741 aaatatttct tgcgcgcggt cataacgcgt ttcaaatcaa caggctcata tggattggtg
    34801 aatacacgct cgtcaaaatc ctgacgcgcg aataaccaaa tttccttttg ccatttgccg
    34861 agtaattcgt ccgcatcctg accttcttca atttggcgca ctaacctcag gaagcgatgc
    34921 tgagttttgt tccagaagtc gatatccaca aaactgaaat caccccgtgc accttttgga
    34981 tcggagaacc acgcttcttt caatgcactt cgtaacagac ttagaatccg tgaagccgtt
    35041 tgcgcagcca gacgcagttt cggtatctgg ccttcttttt tcttgagcag cagcgggaaa
    35101 tggtgttcgt accaacagcg cgctttcatg ttgtcgaaat catagccaaa tccccacagg
    35161 cctacttttg cctgtttcag actgctggca ttaaagagtt tcaccaccag cgcgggaagt
    35221 tccgtattgt tttctgattt acccgtttcg ataaggccta accagtcgcg ccagattaaa
    35281 ccgcccggtt gtggtttgac cgagtaaaac tcgccaccct ctttaagtgg tatacggtaa
    35341 ggcgttaagg gatgctgcca catggcataa ttcgcaccgt aatttttggt cgtcatcagg
    35401 ctcaggagcg cgtcgctctg ttcaccgcaa atatcgcagt tgccgactgt cgtggtatta
    35461 aaatcaatac gaatacggcg cggcattccc cagtaagcct ggagtttatt gacctgatca
    35521 tgggttacca ccgcaccggc cagttcgctg gttcgcgtcg ggccaagcca ggggaaaacc
    35581 agatcgtcaa attttttggg tagcggtaag tcggcttcat cctgcggcat cacattgggc
    35641 cacagtttgc gccacaaggg cgtttgttga ttgccttgat attcctgtaa ttcaatcaga
    35701 gtggtcatcg gcccaccgcc gcgcaaaccg gtgcgatagc ctttgccgcc tgatggcgca
    35761 tttaactgta gggagaacag agctaacgcg gaacaatgag ggcatacgtg ttcagtcacg
    35821 ccacgcttaa taaagtggtc tttattaaac ttcgttgttt gagcgccggg aatctcaggc
    35881 agtagcgaag cgacctgaac tttatctccc ttgagcgcct cgaaatcctg cataaatgaa
    35941 ggggaatctg ccccaaactg gaaagcgtgt tctaaggaca gcaatgcttc ccgtagcttt
    36001 tcagcctcca gtccgtcttg ccagatatca tcccaacgac cgtgattttt gggcgcgaag
    36061 ctggtttgta gtagccccag caaaaactgc caggccgccc cctggagatc tgcccgcggc
    36121 gcagcgatat cgacaacatt ttcatcagcc agatcgactg gtgccagctt gcctgtcgtt
    36181 ccgtctttaa aacgaacagg caaccacggg gttgtcagaa gtgaaaacga gttcatacgc
    36241 atcctctcgt aaaaaagacc atcccaggtc ggttgtccat ctttccatcc ttttcggtgt
    36301 atcaactatt cagagagtcc tgtcgcagac atctcaacca accttgccaa tcaatccttc
    36361 cgtagccgaa tacccgcaac tttcgtcatc gttgactaaa atcacgtttg ccatctccgg
    36421 atcttgtcgc tgttcaatgc accacagcct gaacgcctct ccctccagta aagaaaactc
    36481 atcccgatgt tttttccacc agcttcggcg cactctgaca acgctcattt cccatgcgtg
    36541 agcaccggtg gcataaggct tcaccacacc gtcaatacag gtagccagcc acagggaaac
    36601 agactcctca gccagacgtg tcgacagttt ttccgggagg taatcgttga tattggcggc
    36661 atagccgggc ctgaagttca gcacaaactt tttagccatt gcgcgatcgc agtaatattt
    36721 gcccacttgc tcctgctcgc tgcgggcaaa tccttccggc attaccacgt cctcaccgta
    36781 gacggattca ataagaagcc gggctgcgtg tggcatctga atagcacctt gctcacgcag
    36841 cacacgctgc gtcagccaga ttcgcccatg atcgggatag acaaatgcac tgttacgcat
    36901 ggcactgcca aaccattcgt cgccaggaga gtcgtcccag acgggagcca gaatcagcaa
    36961 ttcagggggg gaacgctcgt cctttccgtc acgctttaac tgaccattaa tatcgcggat
    37021 atgccgctgt aatcgtcctg ctcgctgaat cagcaaatca acaggggcca ggtcggagat
    37081 catttcgtcc aggtcaatat ccacgctttg ctcaattact tgtgtacaaa caatgacttg
    37141 cgacgctctc tgtaaactac aatatttacc aaagcgtgcc agcgtttccg tttcaattcg
    37201 ctggcgatcg ctaaaagcaa agcggctatg aaaaagtgaa aggctggaag caggaatgac
    37261 gccgcgggca agcagctgac gatgaacctt aatagcgtca tcgacagaat tccggatcca
    37321 ggcgatgcat ttcccctgac ttaccgccga ttcgatacgc gcaatacact cttgttcact
    37381 atgaagccaa cccacgctga cgctacgctc aacatctttg cgcgtcgcta cccggtgtga
    37441 gttcacatcg gatttcgtga catgcgtcag ccaggggtaa tcatcctttt ccaggaacgg
    37501 agcttcttgc tggccctctg taccccgcgc aaaggcagcg acgagtttgt cgcgctgctg
    37561 ttgagataac gtggcagaaa gcaaaataac gctgtttccg ccacgcgcct gccgctcgat
    37621 caacccttca agaatgcacg acatgtaagc gtcacaggcg tggatctcat cagccagcag
    37681 gattttgtta ctcagcccca gaagccgcag attattatgt ttgaacggca tcaccgccat
    37741 catcgcctga tccagcgtgc cgacgccaat ttcagccagc aacgccttct tgttactgtc
    37801 ggcaaaccag gccgcacatc cctgactgaa cgtttgttca tccggttctt ctgacccgac
    37861 taaatcacca gaccagagtg attcattaaa gcggtccatt aatgtgcggg cactatgtgc
    37921 cagcaccagg ctggggcggg attctggcga atagaaagca agccaggttt tgaccagccg
    37981 atcgtacatg gcattggccg ttgccattgt tggcagacca aaaaacagcc cctgagcttt
    38041 ccccgcagct atcaacctgt gcgccaggat aagcgccgct tctgttttac ctgcgccagt
    38101 cacatcttcc agaataaata actgcggtcc ttgctggctg atatccagat ccagcacctt
    38161 ttgctgtaac ggtgtcggat tctcaataaa aggaaacagc gtattaattc cgttgaaagg
    38221 cgcgactttt gcttttagag gaaagacggt taaggcgttt tgagcctgaa ttaaagtttt
    38281 ctgccagtaa cctttaatat ccattggatg tgcgacgcgt ggaaaaaatc gcgttgacga
    38341 acccgtccag tctgcgagca cgacggttgc agagatatac caggaaaggt gttttataag
    38401 ttcaatgccc tcatcatcat cccagaatgc ggggatctct atgagcggaa acagtggttt
    38461 gatttcaagg agaaaatctc gtgctgcagc tttgtcttca ggcagaaaat tatccaattc
    38521 atccatacga tcagggggct gaccatgatg ccctgtcgtc acgggcatcc acatttctat
    38581 cactcgccta agtttgcgcg gagagagcga agatgaagga agtaactcct gacattcact
    38641 taaataatga ttccacaacc agtagcccag cgttgaatga gagatttttt cgtaattttt
    38701 tctggcacct tcctgaatct tgagttcagg gggcaggtaa agttgttgaa aagagcgggc
    38761 aaattttcca atatcgtgcc agcacagcaa ccaggcgaaa aattgagccg cctgttcctt
    38821 gtcagaaatc cctaattgac gaaaataatc agccagcccg aagcaatttc ttttaaccat
    38881 taaatagccc atcgcggcta catccagcga atgccagcaa agaaggtgat agtcgtcgcc
    38941 cccgtctttc tcgccgcgtc gggtttttcc ccagaaatca aaaaaagtca caatattttt
    39001 atccttcagt aaac
//
LOCUS       DAZGJW010000029        36962 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZGJW010000029 DAZGJW010000000
VERSION     DAZGJW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.029"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1002
                     /locus_tag="ACHKIK_001552"
                     /pseudo
     CDS             1..1002
                     /locus_tag="ACHKIK_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1045..1245)
                     /gene="glgS"
                     /locus_tag="ACHKIK_001553"
     CDS             complement(1045..1245)
                     /gene="glgS"
                     /locus_tag="ACHKIK_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO9082752.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            1514..2143
                     /gene="yqiJ"
                     /locus_tag="ACHKIK_001554"
     CDS             1514..2143
                     /gene="yqiJ"
                     /locus_tag="ACHKIK_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO9082753.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            2170..3831
                     /gene="yqiK"
                     /locus_tag="ACHKIK_001555"
                     /pseudo
     CDS             2170..3831
                     /gene="yqiK"
                     /locus_tag="ACHKIK_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
     gene            4070..4129
                     /locus_tag="ACHKIK_001556"
     CDS             4070..4129
                     /locus_tag="ACHKIK_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO9082754.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            4446..4505
                     /gene="ibsE"
                     /locus_tag="ACHKIK_001557"
     CDS             4446..4505
                     /gene="ibsE"
                     /locus_tag="ACHKIK_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO9082755.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(4625..6058)
                     /gene="hldE"
                     /locus_tag="ACHKIK_001558"
     CDS             complement(4625..6058)
                     /gene="hldE"
                     /locus_tag="ACHKIK_001558"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO9082756.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(6106..8946)
                     /gene="glnE"
                     /locus_tag="ACHKIK_001559"
     CDS             complement(6106..8946)
                     /gene="glnE"
                     /locus_tag="ACHKIK_001559"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO9082757.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIVHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(8969..10270)
                     /gene="ygiF"
                     /locus_tag="ACHKIK_001560"
     CDS             complement(8969..10270)
                     /gene="ygiF"
                     /locus_tag="ACHKIK_001560"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO9082758.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYHDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            10512..11132
                     /gene="ygiM"
                     /locus_tag="ACHKIK_001561"
     CDS             10512..11132
                     /gene="ygiM"
                     /locus_tag="ACHKIK_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO9082759.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            11196..12434
                     /gene="cca"
                     /locus_tag="ACHKIK_001562"
     CDS             11196..12434
                     /gene="cca"
                     /locus_tag="ACHKIK_001562"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO9082760.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPLTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(12597..13418)
                     /gene="bacA"
                     /locus_tag="ACHKIK_001563"
     CDS             complement(12597..13418)
                     /gene="bacA"
                     /locus_tag="ACHKIK_001563"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO9082761.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(13508..13876)
                     /gene="folB"
                     /locus_tag="ACHKIK_001564"
     CDS             complement(13508..13876)
                     /gene="folB"
                     /locus_tag="ACHKIK_001564"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO9082762.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            13981..14598
                     /gene="plsY"
                     /locus_tag="ACHKIK_001565"
     CDS             13981..14598
                     /gene="plsY"
                     /locus_tag="ACHKIK_001565"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO9082763.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(14611..15543)
                     /gene="ttdR"
                     /locus_tag="ACHKIK_001566"
                     /gene_synonym="dan"
     CDS             complement(14611..15543)
                     /gene="ttdR"
                     /locus_tag="ACHKIK_001566"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO9082764.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            15750..16661
                     /gene="ttdA"
                     /locus_tag="ACHKIK_001567"
     CDS             15750..16661
                     /gene="ttdA"
                     /locus_tag="ACHKIK_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO9082765.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            16658..17263
                     /gene="ttdB"
                     /locus_tag="ACHKIK_001568"
     CDS             16658..17263
                     /gene="ttdB"
                     /locus_tag="ACHKIK_001568"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO9082766.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            17311..18774
                     /gene="ttdT"
                     /locus_tag="ACHKIK_001569"
     CDS             17311..18774
                     /gene="ttdT"
                     /locus_tag="ACHKIK_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO9082767.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(18817..19830)
                     /gene="tsaD"
                     /locus_tag="ACHKIK_001570"
     CDS             complement(18817..19830)
                     /gene="tsaD"
                     /locus_tag="ACHKIK_001570"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO9082768.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            20068..20283
                     /gene="rpsU"
                     /locus_tag="ACHKIK_001571"
     CDS             20068..20283
                     /gene="rpsU"
                     /locus_tag="ACHKIK_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO9082769.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            20394..22139
                     /gene="dnaG"
                     /locus_tag="ACHKIK_001572"
     CDS             20394..22139
                     /gene="dnaG"
                     /locus_tag="ACHKIK_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO9082770.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNLELAKK"
     gene            22334..24175
                     /gene="rpoD"
                     /locus_tag="ACHKIK_001573"
     CDS             22334..24175
                     /gene="rpoD"
                     /locus_tag="ACHKIK_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO9082771.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(24254..24760)
                     /gene="mug"
                     /locus_tag="ACHKIK_001574"
     CDS             complement(24254..24760)
                     /gene="mug"
                     /locus_tag="ACHKIK_001574"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO9082772.1"
                     /translation="MVEDILALGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            24885..24960
                     /locus_tag="ACHKIK_001575"
     tRNA            24885..24960
                     /locus_tag="ACHKIK_001575"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24918..24920,aa:Ile,seq:cat)
     gene            complement(25014..25778)
                     /gene="nfeF"
                     /locus_tag="ACHKIK_001576"
                     /gene_synonym="yqjH"
     CDS             complement(25014..25778)
                     /gene="nfeF"
                     /locus_tag="ACHKIK_001576"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO9082773.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPLSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            26055..26678
                     /gene="nfeR"
                     /locus_tag="ACHKIK_001577"
                     /gene_synonym="yqjI"
     CDS             26055..26678
                     /gene="nfeR"
                     /locus_tag="ACHKIK_001577"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO9082774.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(26832..28352)
                     /gene="aer"
                     /locus_tag="ACHKIK_001578"
     CDS             complement(26832..28352)
                     /gene="aer"
                     /locus_tag="ACHKIK_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO9082775.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            28770..30149
                     /gene="ygjG"
                     /locus_tag="ACHKIK_001579"
     CDS             28770..30149
                     /gene="ygjG"
                     /locus_tag="ACHKIK_001579"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO9082776.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(30191..30523)
                     /gene="ygjH"
                     /locus_tag="ACHKIK_001580"
     CDS             complement(30191..30523)
                     /gene="ygjH"
                     /locus_tag="ACHKIK_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO9082777.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            30742..31725
                     /gene="ebgR"
                     /locus_tag="ACHKIK_001581"
     CDS             30742..31725
                     /gene="ebgR"
                     /locus_tag="ACHKIK_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO9082778.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            31909..35001
                     /gene="ebgA"
                     /locus_tag="ACHKIK_001582"
     CDS             31909..35001
                     /gene="ebgA"
                     /locus_tag="ACHKIK_001582"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO9082779.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQENEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            34998..35447
                     /gene="ebgC"
                     /locus_tag="ACHKIK_001583"
     CDS             34998..35447
                     /gene="ebgC"
                     /locus_tag="ACHKIK_001583"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO9082780.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            35510..36943
                     /gene="ygjI"
                     /locus_tag="ACHKIK_001584"
     CDS             35510..36943
                     /gene="ygjI"
                     /locus_tag="ACHKIK_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9082781.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTVGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
BASE COUNT         9008 a         9944 c         9321 g         8689 t
ORIGIN      
        1 tggactgcta tgctgaacat gaagggggaa ataccgtagt cattggttac gtaccaagaa
       61 tttctatccc cagtaatggt aaaaaaggtg ataagatctg gcaaagcagt gagtatttta
      121 tgaatgtctt ctgtaataat gcattacctg ccccatcacc tggagaagaa tacccatcag
      181 cctggactaa tataatgatg tttttaccag gcggtcagga cttttataat cagaactcct
      241 atatttttgg cgtaacatac aatggagttg attatgattc gactgcccct aatgctatcg
      301 cagcaccaga atgcattgat ataaaaggag cgggtacctt taataatcac tataaaaaac
      361 cccgctgtct gtagtggagg tcctgagcct caattatcag taacctttcc agctcgcgta
      421 caactctata ttaaattagc aaaaaatgcc aatagagtta ataaaaacct ggtgttacct
      481 gatgaatata tcgctctaga gtttaaaggt atgagtggcg caggagctat agaagtagac
      541 aaaaatttga ccttcaggat tcgtggatta aataatattc atgtccttga ctgctttgtt
      601 aatgtcgcac tggaaccagc tgatggcgtt gttgactttg gtaaaataaa ttcccgaaca
      661 attaaaaata ccagcgttag tgagacgttt agtgtagtca tgacaaaaga tcctggtgcg
      721 gcctgtactg agcaattcaa tattttaggg agctttttca ctacggatat tttgagtgat
      781 tatagccatc tggatatggg taatggactg ctattgaaga tatttcataa agatggaaca
      841 gcaacggaat ttaaccgctt ctcacaattt gcttcttttt catcgtctag tgcaccttcg
      901 gtcaccgcac cattcagggc agaacttagt gcgaatccgg cagagactgt tgttgaggga
      961 ccgtttagta aagacgtcat cctcaaaatc acctataact agcatctaat acaaacacta
     1021 aaacgggcca tcaggcccgt tatatcagtg ctctaactcc agctctcttg cctgcatcat
     1081 ctgttgacaa taccaggcat aacgtgcgca aaaccaggtt ctatgttctt catccatgtt
     1141 accagtaacg gcaagaatat cgaccgggcg accttctgcg tgcattctgg caaaactacg
     1201 cgccaggaaa tcgaaattat ttaaagaatt aagactatga tccataagca catcctccgg
     1261 ttatcgcata ccttttaagg ccatatgctt tttctgattt cagtattccc caatcagtgt
     1321 agtgccttta aacataacgt gcgtaaatat attgtccccg atcacacttt ttagtgaaaa
     1381 ataaaaaacc atattaaaaa actttctctt ccagaacttc tctaacatgt gttattatta
     1441 acaaagataa atgtaagcaa ggaaactatc ccaaagtaaa tattgcgtca ctaaatggac
     1501 attggagtga catatgattt tattcgccga ctataatacc ccttatcttt tcgctatttc
     1561 ctttgtactg ttgatcggcc ttttagaaat tttagcgctt atctgcggtc acatgctctc
     1621 cggtgctctt gatgcacatc ttgaccatta tgattccata accacaggtc atattagcca
     1681 ggcacttcat tatctcaata tcggaagatt gccagctctc gtcgttctct gcttgctggc
     1741 tggttttttt ggtcttattg gcatcctgtt gcagcatgcc tgcatcatgg tctggcaatc
     1801 accactgtcg aacctgttcg tggttcctgt cagtttactg tttacgatta ttgcggtgca
     1861 ttataccggt aaaatagttg cgccctggat tccacgcgac catagttcag ctattacaga
     1921 agaagaatat attggtagca tggcattaat taccggtcat caagccactt caggtaatcc
     1981 ctgcgaagga aaactcaccg atcaatttgg ccaaatacat tatttactac tggagcctga
     2041 agaaggaaaa tttttcacta aaggagacaa agtgttaatt atttgccgac tttctgcaac
     2101 gcgatatttg gcggaaaaca atccctggcc acaaatattg taatttatta atcaaaagga
     2161 aatttaataa tggatgatat tgttaattct gtgccctcct ggatgtttac cgcgattatt
     2221 gccgtatgca ttctgtttat tattggaatt attttcgcca ggctctatcg tcgcgcttcg
     2281 gcagagcaag cttttgttcg tactggttta ggtgggcaaa aagtggtgat gagcggtggc
     2341 gcaatcgtga tgccgatctt tcatgaaata atccccatca atatgaatac tctgaagctg
     2401 gaagtcagcc gctcaaccat tgatagcctg attacgaaag atcgtatgcg tgtcgatgta
     2461 gtagtcgctt tctttgtacg ggtaaaaccg tctgtagaag gcatcgccac tgcggcgcag
     2521 acgttggggc aacgcacgct atcaccagaa gacttacgta tgttggttga agataaattt
     2581 gtcgatgccc tccgtgcaac agctgcacaa atgaccatgc atgagttaca ggatacccgc
     2641 gagaacttcg tgcagggagt acaaaatact gtcgcagaag acctgtcgaa aaacggtctt
     2701 gagctggaaa gcgtttcact taccaacttt aaccagacct cgaaagaaca tttcaatccg
     2761 aacaatgcct ttgacgccga aggtttaacc aaactgactc aggagacgga gcgccgtcgt
     2821 cgcgaacgta acgaagttga acaggatgta gaagttgcgg tgcgtgagaa aaaccgtgat
     2881 gcactttcgc gcaagttgga gattgaacaa caagaagcgt ttatgacgct tgagcaggag
     2941 cagcaggtta aaacccgtac tgccgaacag aatgcacgta ttgcggcttt tgaagctgaa
     3001 cgtcgtcgtg aagcagagta gacgcgaatt ctggctgaac gacaaattca ggaaacggag
     3061 atcgagcgcg agcaggccgt ccgctcaaga aaggttgagg ctgaacgtga agttcgtatt
     3121 aaagagatcg aacaacagca ggtcaccgaa atcgccaacc agacgaaatc gatcgctatt
     3181 gccgccaaat cggaacaaca gtcccaggca gaagcgcgtg ctaaccttgc actcgcagaa
     3241 gcagtaagtg cgcaacaaaa cgtagaaacc actcgccaga ccgcagaagc cgatcgtgct
     3301 aaacaagttg ccctaatcgc tgccgcgcag gatgcagaaa ccaaagcggt tgaactgacc
     3361 gtgcgggcga aagcagaaaa agaagccgca gagatgcagg cggcggctat cgttgagtta
     3421 gccgaagcta cacgtaaaaa gggtctggcg gaagcagaag cacaacgtgc gctgaacgat
     3481 gctatcaacg tactttctga tgaacaaacc agccttaaat tcaaactggc cttgttgcag
     3541 gcgctacctg cggtaataga aaaatccgtt gagccgatga aatctattga cggtatcaag
     3601 attattcagg tcgatggtct gaatcgtggc agcgctgcgg gtgatgcaaa cacgggtaat
     3661 gtggggggcg gaaacctggc ggaacaagca ttatcagccg ctctctctta ccgcacacag
     3721 gcaccgctga ttgactcctt gctcaatgaa attggcgttt caggcggctc actggcggca
     3781 ttgacttcac ccttaacctc aacaactccc gtcgccgaaa acgtagaata aattttcagc
     3841 tatgaacgtc cctgtcacaa aaagccgggg attttttata tctgcgttcc gctaaaaact
     3901 gcaaatgatc aggtcgttgc aacgttttgc gtgaccgctc ggggaaggca aaactgcctc
     3961 tgggaaagcg ttgcgcgagg tccggcgctc atcaacaatc ggggagtcag caaagggctg
     4021 aaacgggaaa gcccctccct aggaaggggc cttacagaag gaaagggtta tgatgaagct
     4081 cgtcatcata ctgattgtgt tgttaatcgt aagtttcgca gcttattaac agccaatcag
     4141 aggggggaga aatcctccct cacccttgtt cctttacttt aggttgaaaa aaaatcagcg
     4201 tcaagaaggt ttcattaaca catatccctg gcccaaaaag ccggggattt tttatatctg
     4261 cgttccgcta aaaggtgcaa atgctcagga cgttgcagcg ttttgcgtga ccgctcgggg
     4321 aaggcaaaac tgcttcaggg aaagcgttgc gcggggtccg gcgctcatca acaatcgggg
     4381 agtcagcaag gggctgaaac gggaaagccc ctcccgagga aggggcctta tagaaggaaa
     4441 gggttatgat gaagttcgtc atcatactgg ttgtgttgtt actgttaagt ttcccgactt
     4501 actaacaact gatcagaggg gggagaaatc ctcccttacc cttgttcctt tactttaggt
     4561 tgaaaaaaca acagcgtcaa taggcctgcc tgctacgaag cgagatctgt gaaccgcttt
     4621 ccggttagcc ttttttatcc tgttggatct tcttgatgat gttagtggtc gagcaaccgt
     4681 cttcgaagtt gagcaccagc acttcgccgc cgttggccca gacttcttta ctcccggcga
     4741 tctcttctgg tttatagtcg ccgcccttca ccagtaaatc tggcaggatc ccggcgatca
     4801 agcgctgcgg cgtgtcttct tcaaacgaca ccacccagtc gaccgcttcc agtgcgccca
     4861 gcacaatcat acgctgttcg agcgggttaa cggggcggga atcccctttc agccgtttgg
     4921 tggaggcatc gctgttcaca gcgacaatca gacggtcacc cagtttgcga gcatttgcca
     4981 gataggagac gtgcccggcg tgcaggatgt caaagacacc gttggtcatt accacctttt
     5041 cgccgcgttt acgcgctgcc gctacagcaa gcttcagttc ctcttcggtc atcacaccaa
     5101 agccggtatc tgcgcgccca cgcactgcat tttccagctc gatcggcgaa accgtggagg
     5161 ttcccagttt gccgaccacc acgccagccg ccgcattggc aaagaagcag gcttcttcca
     5221 gcgaattacc cgctgccagc gttgccgcca ggacgccaat caccgtgtcg cccgcaccgg
     5281 taacgtcata cacttcctgc gcctgggttg gcatatgcag cggcgcttta cccggttgca
     5341 gcagcgacat accctgttcg gaacgggtca ctaacagagc cgagagttcg taatcggcaa
     5401 tcagtttcat gccgcgctca acaatctctt cttcggtctt acatttaccg acaacagctt
     5461 caaattccga gagattcggt gtcagcagcg tagcgccgcg gtagcgctca aaatcggtac
     5521 cttttggatc aatcagcacc ggaacgcccg ctttacgcgc cagttggatc atctgctgta
     5581 cgcttgccag cgcacctttg gcgtagtcag aaagcaccag cgcgccaatc gaactcagcg
     5641 cctgattaat ccgctcgtgc agcggctgcg gatcaacacc ttcgaaacct tcttcaaaat
     5701 ccaggcggat cagctgttgg ttgcgggaaa gtacccgtaa tttggtgata gtcggatgcg
     5761 tcggtacaga aacgaagtcg catttgacgt tgacgtcggc cagagattta ctcagcgcac
     5821 gcgccgcatc gtcaatgccc gtcagcccga ccaggcgagc attggcaccg agagaagcga
     5881 tgttcattgc cacgttagcc gcgccgcccg gacgttcttc gatggtattg actttaacca
     5941 cgggcaccgg cgcttccggc gagatacggc tggtgggacc gtaccagtaa cgatccagca
     6001 tcacatcacc aaccaccatc actcctgcac gttcaaactc tggcagcgtt actttcattc
     6061 ctgtctcctg agagattcaa aatttgcgcg cgataatacc atacttcatt cttccaccag
     6121 ccacttctgc cagcttgccc gcaccagttc acgctctgcg gtgaagcaat cctccggcac
     6181 atggcccggc aattcctgta atgccagatg atgaagttca tcgcgcagcg tagtatacgc
     6241 acgggtcagc gccatcgctt cctgctcttc cataatgtcg ttttgcgcca gtagttccag
     6301 aatgcgcacg ttgtctgacc agcgcgttaa cttcggtttt tcatgagcgt agcgcaacac
     6361 cagatattgg gtaataaatt cgatatcggt aattccgcct tcatcagctt tgatatcaaa
     6421 gcgatcgcga tgtttattgc cgagatgagc gcgcattttc tcgcgcattt cccgcacttc
     6481 cgtttgcaga gttttacctt cacgcggcag cgtcataatc tcgcgacgca ccgcgtcaaa
     6541 gtgcgcggtg agctgcggat cgccgtacac tacccgcgca cgcaccagcg cctgatgttc
     6601 ccacgtccag gcctcgtttt tctgataatc ggcaaatgct tctgcggatg tcaccagcat
     6661 tcccgcggcc ccggacgggc gtagccgtgc atccacttca tacaaaatgc cggaagaggt
     6721 acgcgtactg aacagatgca taatgcgttg cgccagacgc agataaaact gtcgcccgtc
     6781 gatttcccgc tcgccgtcag tcatcgcatc cattgggcaa tcatggagga agatcaggtc
     6841 aagatcggaa ctgtagccta actcccagcc gcccagcttg ccgtagccga ccaccgcaaa
     6901 accacggcct tcgcgatcgt tgaggtgatt tggcttaccg tagcgggcca ccatttgaac
     6961 ccacgcctgc tgaacgacgg catctatcat agcctctgcc agccaggtta agtgatcgct
     7021 cactttcatc accggtagcg taccagcgat atccgctgcg gcgatgcgta acagctgtgc
     7081 ctgtttgaac tgacgcagcg cctcaagctg ttgctcttcg tcatcttccg gcacgcgcag
     7141 caaatactgg cgcaactcat cgcggtaggc atcggtcgcc gtcggctggt aaagggtgtt
     7201 tggatcgagc aattcatcca gcaataatgg ataacgcgcc agttggctgg caatcatcgg
     7261 cgacgcggca cacagagaaa tcaaatgttt aagcgccgcg gggaattcac tgagcaattc
     7321 taaataggtg gtgcgggtaa caatccccac cagcaaggcg gtaatgcgcg acagcgtaac
     7381 ggcagcgtct tcacgcgcac agacatcact tagcagatgc ggcatcagat ggtcgagcac
     7441 ctgacgtcct cgcggcccga tggtgcgctt atccagctct ttgcggaaat cggcaatcag
     7501 cgttagcacc tgtttgcgat catcctcgct aagatgcgcc agcactggcg tagtgtcatc
     7561 ttcctgcaac gcatcctgcc acagctcacg ccactgttcc gacagcgact cttcctgagt
     7621 ttcactttcg tcgtcgccaa tcaattcatt aaacacccgg cgcacattgg tcatatgtgc
     7681 ggtcagcgcc ccggtcagtt gcggccagtc agcaaagtcc atcgcccacg ccagccgcgc
     7741 acggttaagc tcatcagaag gaagcgtctg ggtttgttcg tcgttaatgc tttgcagcag
     7801 gttttccaga cgccgcagga acagatacgc cactcgcaat tgttcagcat cgttttcaga
     7861 aagcagatgc agcgcggcaa tgacgctgag cgttggcagt aaagagcgcg actgcagcga
     7921 cggttcgcgc ccaccgcgaa tgagctggaa cacctgaacg ataaattcaa tttcgcgaat
     7981 gccgcctgcg ccgagtttga tattgtcggt caaaccacgt cgacgcactt cacgggcaat
     8041 catccctttc atgttgcgca gcgactgaat cacgctgaaa tcgatgtaac gacggaaaac
     8101 aaacgggcgc agcatcgcac gcaactcgtt agcatagacg ccttcgctat cgcccataat
     8161 ccgcgccttg accatcgcgt aacgctccca gtcgcgcccc tgctcctggt aataatcctc
     8221 caacgcggca aagctcagca ccagcgggcc actttcgcca aacggacgca gccgcatatc
     8281 cacgcgatag acgaagccat cctgcgttgg ttgatccaac actttaatca gccgctgccc
     8341 catgcgggta aaaaactgcg cgttatccag ttcccggcgt ccgccctgcg tacaaccatg
     8401 ttccggccag gcaaaaatca gatcgatatc tgaggagaaa ttcagttccc caccgcccag
     8461 cttacccatg cctaaaatca gcagcggttg cgcttcgccc tgcgcattgc acggcgttcc
     8521 ccactcgcgg cagcaagcgt catacagcca gtcacgcgcg gcgacaatca gcgtctccgc
     8581 cagatggctg agctgctgca atatgctctc ttcagtaacc aaagccagcg tttgcgccca
     8641 ggcgatgcgc accataatgc gccgccggaa tagccgcagc tcgcgcatta acccggcttc
     8701 gtcactcaca ttactgagcg cctcctgcaa ccatgccgcg taatgctgcc attcgtcggc
     8761 ctgcggcggt tggctttcca gttccgtcag ccactctgga tgcacaatca cgctgtcctg
     8821 cacaaaatca ctaaaagtaa gtactgactt cgcctgtgcg ctaagtgatt cctcggctaa
     8881 aggctctggc agccgctcaa caacggtctg ccagtactgc tgtaacggtg aagagagcgg
     8941 cttcataagc gattttatcc ttgcctgatt aacgttttcc gctgtgcaac cagaacggtt
     9001 cctgattgtt tgcctcatta cggaaatgtt caatttcaat gcgttgcccg gtcgcaatag
     9061 cgtgacgcag cccctgccag ttctccagcc aggcttgcgc gacgacagga tcatagtaac
     9121 ctgccagcag cagtattgag tcaatatcac gcgtcaggcg tggcaactga tcatggtagc
     9181 gatcgcctaa cggctggcag aaaacgcttt tcagttcagc ggcatggcgg gaaagatgga
     9241 tatcggcaaa gcgtttgaag gaatcgctga ttttaccctg cgctttggca tctaaaaacg
     9301 gctgccaggc tttgcttacc aaccattcgg tcaacgccag ctttgccatt gccgtttcgg
     9361 tagagtagac cgccgtcacg gcagaaaccg cagaagcgat ggtcgcttcg cattgagtca
     9421 gcaaatcacg taagtgagtg ctcgctttac gcggcacaat gccaccgaac agcatcagtg
     9481 catggcgaac aaggccaatg gctgccagca cctgctcttt cgccgcatcg ttgccgcgta
     9541 cccacagttc ttcatgatac tgccattgcg ctaacgccag ctcgaacgcc gcttccagcc
     9601 cctgttccac atcggctttt gccgcaacat gcaaaatggt ggtcggtttg atttcacgcg
     9661 ccggattgcc ctgcgccaga tgatagccac gcgccgcttt gctcaggctg ccctggcgta
     9721 atccggtttg cgataccagt tggttcgcca gtttcagcac cgcgcgcgtg tcgccgctaa
     9781 gcagttccag ttccagctca cagataggtt cagcaaattc ccccgctttc acctccccct
     9841 gatcgagagc gatttcaatt cggctgtcat cgaccgccac cagccatttt tcgcgataaa
     9901 aatcggtgct gaacagcggt tgtacgcggg aagcgagatc ggcgggcaat tcgccgttcg
     9961 gccagacttc cgtcggtaac tgcgccaggt cgagcgtcgg ttcgctcaac gccacgttat
    10021 attccgggcg ctggtgtaag ccgcctgtca ctcttcctgc aactttcatg gtcatctcat
    10081 agcgaccgtt ttcgccacga atacgtaagc ccatatcgtg cccacgcagc cagttatccg
    10141 gcgtttcgta gtaaatattc agcaactgca cggggtcatg gtgctcgccg cccagcgtat
    10201 tgagatggtc acgcaacgcc tcaacggcac tgtgattaac aataaacttt aattcgattt
    10261 cctgagccat ggcctttact tatcggttat gtcacataag tgacgatgaa cggcgaactt
    10321 aatgcgatct tttttgtcag tagatagtat tttgcgccaa attgccatgc aacgggcaat
    10381 ttgacgggcg taaaagtttg aagcagtggc aagacctgac acagaggatt ccgattgatg
    10441 tcgaatgctt tgcgtagcga cactgatgcc actactatcg ttccactttc catgacaata
    10501 acgacagcct gatgccaaaa ttacgcctga tcggattaac tttactcgca cttagcgcca
    10561 ctgccgtctc acacgctgaa gaaacgcgct atgtttctga tgaactgaat acctgggtcc
    10621 gtagcggtcc gggagatcat tatcgcctcg tgggcacggt taacgccggc gaggaagtga
    10681 ccttattaca aactgacgcc aacaccaatt atgcccaggt gaaagacagc tctggccgta
    10741 ccgcctggat cccgttgaaa caacttagca ctgagccaag cctgcgctcc cgtgtgccag
    10801 atctggaaaa tcaggtcaaa accctgaccg ataaactcac caatatcgat aacacctgga
    10861 atcagcgcac cgcagaaatg cagcaaaaag tggcgcagag cgacagcgtg atcaacggat
    10921 taaaagaaga aaatcagaaa ttgaaaaacg agctgattgt cgcgcagaaa aaggtcgatg
    10981 ccgccagcgt acagttggat gacaaacagc gcaccatcat catgcaatgg tttatgtatg
    11041 gcggtggcgt gctggggctt ggcttgctgc tcggtctggt actgccacac ctgatcccaa
    11101 cgcgcaaacg caaagatcgc tggatgaact aaatcgcctt cttcgttgca ctgtcttatt
    11161 attaggaatt gtgtatttga cgaagggatg atggcgtgaa gatttatctg gtcggtggtg
    11221 ctgttcggga tgcattgtta gggctaccgg tcaaagacag agattgggtg gtggtcggca
    11281 gtacgccaca ggagatgctc gacgcgggct accagcaggt aggccgcgat tttcctgtgt
    11341 ttctgcatcc gctaacgcat gaagagtatg cgctggcacg taccgaacgg aaatccggtt
    11401 ccggttacac cggttttact tgctatgccg caccggatgt cacgctggaa gatgatctta
    11461 agcgtcgcga tctgaccatt aatgcgctgg ctcaggacga taacggcgag attatcgacc
    11521 cgtacaacgg tctgggcgat ctgcaaaatc gtctgttgcg ccatgtttcc cccgcttttg
    11581 gcgaagatcc gttacgcgtt ctgcgcgtgg cgcgttttgc tgcgcgttat gcccacctcg
    11641 gttttcgtat tgccgatgaa actctgacgc tgatgcgcga gatgacccat gcgggtgaac
    11701 tggaacacct gacgcctgaa cgggtatgga aagagacgga aagcgccctc accacccgca
    11761 atccccaggt gttcttccag gtactgcgcg attgcggtgc acttcgcgtt ttattcccgg
    11821 aaattgacgc cctgtttggc gttccggccc ctgccaggtg gcatccggaa atcgacacgg
    11881 gtattcatac cttaatgacg ctctcaatgg cggcgatgct gagtccgcag gttgatgtcc
    11941 gtttcgcaac tttatgccac gatctcggta aaggactgac gccgccagaa ctttggccgc
    12001 gtcatcatgg gcatggcccg gcgggtgtta agttagtgga acaactatgc cagcgtctgc
    12061 gcgtgccaaa tgaaattcgc gatttagcca gactggtggc tgagtttcac gatctcatcc
    12121 acaccttccc aatgctgaac ccaaaaacca tcgtcaaatt atttgattcc atcgatgcct
    12181 ggcgtaaacc gcagcgtgtc gagcaactgg cgctgaccag cgaggctgac gtacgcggca
    12241 gaaccggttt tgaatcagcg gactacccgc aaggccgttg gttgcgcgaa gcctgggaag
    12301 tggcgcagtc agtgccgaca aaagcggtcg ttgaagcggg atttaaaggt gtggagattc
    12361 gcgaggagct gacccgacgg cggattgcgg cggtagccag ttggaaggaa caacgttgcc
    12421 caaagcctga ctgagtataa aactaaacgt taacacgaac catcccctcg ccccttcggg
    12481 gagagggtta gggtgagggg aacagaccag caccggtgcg aacaatcacc cccaccccgg
    12541 ccctctccct acagagcgag ggagaaaacc aggcctgctc ccgaaccttt tctcaatcaa
    12601 aagaacacga cgtacaccgc agccgccaca ataaagcgat aaatggcgaa cgggataaac
    12661 gaaatgcgct taatcaattg caggaaggtt ttaatcgcta tcagcgccac cacaaaagcg
    12721 gtgataaacc caacggcaaa catcgggata tcgccggttg tcaggaagcc ccagcttttg
    12781 tagagatcga gcgccgttgc gcccatcatc atcggtaccg ccagcaggaa cgagaactcg
    12841 gaagcagcgt aacggctcac gcccatcagc atcccacctg aaatggtggc cccggaacgg
    12901 gagaaacccg gccacagcgc cagacactgg aaacagccaa tcataaacgc ctgacgatag
    12961 gtcatatcat caagacccgg cgcacgcggc tctttcggct tcaggcattc ggcggcaatc
    13021 agcaacaaac cgccaacgac cagtgcatac atcacattta tcgggttaaa caatgactta
    13081 atcgtgtcgt ggaacaacag ccccagtact accgccggaa tcatccccag caaaatgtgg
    13141 atcagcgtta aacgaccttt gctttctcct tcgtgctgca acgggcggcc aaagtggatg
    13201 ccaatcaggc caaacagacg ccgccagaac atcactacta ccgccagaat tgatcctaac
    13261 tggatcacaa cttcaaaggt tttcgccgtg tcgccctcga accccaacaa gtgaccgaca
    13321 ataatcatat ggcccgtgct ggataccggc agaaattctg tcaatccttc gaccacaccc
    13381 aatattgccg ctatcagcag cgagtgcata tcgctcatca ataaacccct aaattattaa
    13441 aatgtaccgc ttgtccgaac tactgcgtat gaccaggtta taaccgtttg gtttaacagc
    13501 tgtaaaatta attattttct ttcagattat tgccacgctc aatgattacg ccaacattcg
    13561 ccgcccgcgc cactgcgcct ggcttgctga gtttgatacg cacccacggc gagttaaagc
    13621 gtgctaacag cagctccgcc acctcttcag ccacgcgttc caccagcgca aaacgcgccc
    13681 cctcgacgtg gctgaccacc gtttctgcaa tgtcagcgta actgaggcaa tccgccacat
    13741 catcactttt cgccgcttta cggttatccc atgccatttc gatatcgaac actaacttct
    13801 gttcgatggt ctgttcccag tcgtaaacac caatagtggt gattaccgaa agttgctcta
    13861 taaatacaat atccatcacg tcctgcctgc tttttggcta accggatacc acttccggcg
    13921 aaatgtgcgt attatccaca gattcatcgt tgaacacgaa ttttcaaaac ggaacagctt
    13981 atgagtgcaa tcgcgcctgg aatgatcctc atcgcgtacc tctgcggctc catttccagt
    14041 gccattctgg tttgccgctt gtgtgggctg cccgatccgc gaaccagcgg ctccggcaat
    14101 ccaggcgcaa ccaatgtgtt acgtatcggt ggcaagggag cagccgtagc agtactgatt
    14161 ttcgacgttc tgaaaggaat gttgcccgtc tggggcgcgt atgaattagg tgtcagcccc
    14221 ttctggctag gcttaattgc catcgccgcc tgtcttggac acatctggcc cgttttcttc
    14281 ggatttaaag gaggaaaagg cgttgctacc gcttttggtg ccatcgcacc cattggctgg
    14341 gatctcaccg gagtaatggc gggaacctgg ttactgaccg tgctattgag cggatactcg
    14401 tcgctgggag cgattgtcag tgcactgatt gctccgtttt atgtatggtg gtttaagcca
    14461 caattcacct tcccggtttc gatgctctct tgcctgatcc tgctgcgtca tcatgacaac
    14521 atccagcgtc tgtggcgtcg tcaggagaca aaaatctgga cgaaattcaa aagaaagcgc
    14581 gaaaaggatc ccgagtgatt tctggtggat ctacatgacc tgatagcctt catcgggctt
    14641 gcccagccgt tgctggcacc atgccgccag aaattccacg cagacacgta atttcatgct
    14701 gcgatagagc ggctcccggt aaacagccca gatattggcg ctttgtgcat actccggcaa
    14761 tacttgcacc agtttgccac tctccagaaa cggcaacaca tcccactcgg aacgcagcat
    14821 aatccctttg ccctccagcg cccattgcaa cactatctcg ccgctattgg aggaaagatg
    14881 cccgctaacc ttcaccgatt ttttctcctg accgttcccc aactcccata ttccgtgggt
    14941 catatcgcgc tctttggtca ccagacagtc atgacgactt aattcctgta atgattgtgg
    15001 ctgcggatat ttttgcagat actcaggtgc tgcgcataat attcttttat ttttcgttaa
    15061 cagatgcgca atataataat caggaatttc gtcattaata cgaatatcca gatcaatatt
    15121 atcctgcact aaatcaattt gccgatcgaa caattcaaaa tgcacctgta actcaggata
    15181 attgcgcata agttcggtaa tggctggcgc aatatggctg cgcccaaaac caaaactgca
    15241 accaatacga atcatcccct ccgggcgcgt tttgatttgc gtgacgtcat cgaccagtcg
    15301 ctgatattgg gtgaggattt ccagcgcatg ttcgtaacaa cgttggccac tttcagtcag
    15361 cgccacgccg cgagcggagc ggttcagcag cgttgttgcc agcgtatttt caaggatttg
    15421 gatccgcttg gtgacaaatg caggcgtctg cccaagcgtc gccgctgcag cactaaaact
    15481 cccgctgtgg acgatttcta ccagtacctg caagtctttg gctaaaggcc agctattcag
    15541 catcgttaat tatccgcagt tgtgataagc gcagtgtatt tcgcaaaaca ttgccattcc
    15601 ctgctctggc gcaatattca ttcttatttt cgcgttgatt cacgaactgt taattacata
    15661 ttcacggtgg caaaaaatat aaaaccacat ttttagtggt agtttgtggc ggtgaatttt
    15721 tccagacaaa tacaaaaact ggagttgcca tgatgagcga aagtaataag caacaggcag
    15781 tgaataagtt gacagagatt gtcgctaact ttaccgccat gatttctacc cgaatgcctg
    15841 atgacgtggt ggataaacta aaacagctaa aggatgccga aacgtcgtcg atggggaaaa
    15901 ttatctacca tacgatgttc gacaacatgc aaaaagcgat tgacctgaat cgtcctgcct
    15961 gtcaggacac cggggagatt atgttcttcg ttaaagtcgg ttcccgcttc ccactgcttg
    16021 gcgagctgca aagcatactc aaacaagccg tggaagaggc aaccgtcaaa gcgccactac
    16081 gtcacaatgc ggtagaaatt tttgacgaag taaacaccgg caaaaatacc ggtagcggcg
    16141 taccgtgggt cacctgggac atcatccccg acaatgacga tgcggaaatc gaagtttaca
    16201 tggcaggcgg cggctgcacg ctacctggcc gctcgaaagt gttaatgccg tcagaaggct
    16261 acgaaggcgt ggtgaaattc gtcttcgaaa atatctccac cctcgccgta aacgcctgtc
    16321 caccggtact ggtgggcgtg ggcatcgcca cctcggtgga aaccgccgcc gtgctctcgc
    16381 gaaaagccat tttgcgcccg attggcagcc gccatcccaa tccaaaagcg gcagagctgg
    16441 agctacgcct ggaagaagga ctcaaccgtc tgggaattgg cccacaaggg ctgactggca
    16501 acagttcagt gatgggcgtg catatcgaat ctgccgcccg ccatccgtca accatcggcg
    16561 ttgctgtttc tacaggctgc tgggcgcatc gtcgcggcac actgctggtt catgccgatc
    16621 tcacctttga aaatctgtct cacacccgga gcgcgttatg aaaaagatcc tgacaacccc
    16681 gatcaaagct gaagatctgc aagatattcg cgtcggcgat gtgatctacc tgaccggtac
    16741 gctggtgacc tgccgtgacg tttgtcaccg ccgcttgatc gaactgaaac gtccgatccc
    16801 ttacgatctc aacggcaaag cgattttcca cgctggcccc atcgtgcgca aaaacggcga
    16861 taaatgggag atggtttccg ttggcccgac aaccagtatg cgtatggaaa gttttgaacg
    16921 tgaatttatc gagcagaccg gcgtgaaact ggtggttggc aaaggtggta tggggccgct
    16981 gaccgaagaa ggctgccaga aattcaaggc gctacatgtg attttcccgg caggctgcgc
    17041 ggtgctggcg gcaacccagg tagaagagat tgaagaagtg cactggacag aactcgggat
    17101 gccggagtca ctgtgggtct gccgggtcaa agagttcggc ccgctgattg tctctattga
    17161 tacccacggc aacaacctga tagccgaaaa caaaaagctg ttcgccgaac gccgcgatcc
    17221 catcgtggaa gagatctgcg agcacgtcca ttacatcaaa taaccctccc ggagaggctc
    17281 acccctctcc tttttcgcag gcataacacg atgaaacctt ccactgaatg gtggcgatat
    17341 cttgcgccgc tggcggtcat cgccattatt gctctacttc cggttcccgc agggctggag
    17401 aaccatacct ggctctactt tgccgttttt actggcgtga tcgttggact gatcctcgaa
    17461 cccgtgccgg gtgccgtggt ggcgatggtg ggtatctcca tcatcgccat actctctccc
    17521 tggctgctgt tcagccccga acagctcgcc cagccaggct ttaaattcac tgcaaaatcc
    17581 ctctcgtggg cggtttccgg tttttctaat tcggttatct ggctgatttt cgccgccttt
    17641 atgtttggca caggctatga aaaaaccggg cttggacgcc gcattgcgct gtttctggtg
    17701 aaaaagatgg gacatcgcac gctgtttctc ggctatgcgg tgatgttctc cgagctgatc
    17761 ctcgcacctg taacaccgtc caactcggcg cgtggtgcgg ggattatcta tcccatcatc
    17821 cgtaacctgc caccgctcta tcaatcacaa ccaaacgaca gcagttcgcg cagcattggc
    17881 tcgtacatca tgtggatggg gattgttgcc gactgcgtga ccagcgccat tttcttgacg
    17941 gcgatggcac caaacttgct gttaattgga ctgatgaaaa gcgcatctca cgccacgctg
    18001 agttggggcg actggttcct cgggatgttg ccgctcagta ttttgctggt gctgctggtt
    18061 ccctggctgg cttatgtgct gtacccgccg gtactgaagt ctggcgatca ggtgccgcgc
    18121 tgggcagaga cggaactgca ggcaatgggc ccgctctgtt cgcgtgaaaa acggatgctg
    18181 gggctgatgg taggcgcgct ggtgctgtgg attttcggcg gtgattatat cgatgccgcg
    18241 atggtcggtt acagcgtggt ggcactgatg ctgcttctgc gcattatcag ttgggacgac
    18301 attgtcagta ataaagcggc gtggaacgtt ttcttctggc tggcctcgct tatcaccctc
    18361 gctaccggac tcaacaacac tggttttatt agctggtttg gcaaactgtt agcaggcagc
    18421 ttaagcggtt attcgccaac gatggtgatg gtggcgttga ttgtggtgtt ttatctgctg
    18481 cgctactttt tcgccagcgc cacggcgtat acctccgccc tcgcaccgat gatgattgcc
    18541 gctgcgctgg cgatgccgga aatcccgctg ccggtgttct gcctgatggt tggcgcggca
    18601 attggtctgg ggagcattct tacaccatac gccaccggac ccagtccgat ttactacggt
    18661 agtggttatc tgccaacggt ggattactgg cgactggggg cgatttttgg actgatattc
    18721 ctcgtgttgc tggtgattac cggcttgctg tggatgcccg tggtgttgct ttaagtgagg
    18781 acggtgccgg aaattgctct ccggcaccgg atagttttac gcagccggta actctgccag
    18841 tggccagcgc ggacgcacgc taacgccgag atccgccgtc gcgcctgctt taaaccgcac
    18901 cattccggca taggcgatca tcgcgccgtt atcagtacaa aattccggac gcgcgtagaa
    18961 cacttcgccg cggcgttttt tcatcatttc cgccagcttc gcccgtaacg tgcggttagc
    19021 actcacgccg cccgccatga ccagtcgctt aaagcccgtc tgatccaacg ctcgcttaca
    19081 cttaatcatc aacgtatcga ccaccgcatc ttcaaaggcg cgggcgatat cagcacgcgt
    19141 ctggtcgtcg gtgccgttgt cacgaatcgt gttcgccgca aaggttttca gaccagaaaa
    19201 gctgaaatcc agccccggac ggtcggtcat cggacgcggg aaaacaaagc gcccggcagt
    19261 accctgagcc gccattttcg acagtaacgg tccgccagga taatccagcc ccagcagctt
    19321 cgcggtttta tcaaacgctt caccggcggc atcatcgata gactcgccga gcagctcgta
    19381 ctgaccaatg ccagtcacgc tgattaactg cgtatgaccg ccggacacca gcagcgcgac
    19441 aaacggaaat tccggcgggt tatcttccag catcggcgct aacagatgcc cttccatatg
    19501 gtgtaccggg attgccggaa cgttccaggc aaacgccaga gaacgcccca cggttgcgcc
    19561 aaccagcagt gcgccgacta atccagggcc tgcggtatag gccacagcat caatgtcttt
    19621 tgccgttaaa ccagactcct ttagcgccgc ctggatcaac ggtacggttt tgcgcacgtg
    19681 atcgcgggag gccagttcag gcacgacgcc gccgtagtca gcgtgcaatt tcacctgact
    19741 atacaattgg ttggctaaca aacctttttc atcgtcgtaa atggcgatgc cggtttcatc
    19801 gcaggaagtt tcaataccca gtacacgcat gacttgtttt acctcgcttt attaccgcgc
    19861 agtgtaggac caatgcgggt tgatgtaaaa ctttgttcgc ccctggagaa agcctcgtgt
    19921 atactcctca cccttataaa agtccctttc aaaaaaggcc gcggtgcttt acaaagcagc
    19981 agcaattgca gtaaaattcc gcaccatttt gaaataagct ggcgttgatg ccagcggcaa
    20041 accgaattaa tcaaaggtga gaggcacatg ccggtaatta aagtacgtga aaacgagccg
    20101 ttcgacgtag ctctgcgtcg cttcaagcgt tcctgcgaaa aagcaggtgt tctggcggaa
    20161 gttcgtcgtc gtgagttcta tgaaaaaccg actaccgaac gtaagcgcgc taaagcttct
    20221 gcagtgaaac gtcacgcgaa gaaactggct cgcgaaaacg cacgccgcac tcgtctgtac
    20281 taattccccg agagcgttgc tctccgatca gaccgagttg tagttgtaag gccgtgcttc
    20341 cgaaaggaat gcgcggctta ttttcgttta tgaattgcta aaaatcgggg cctatggctg
    20401 gacgaatccc acgcgtattc attaatgatc tgctggcacg cactgacatc gtcgatctga
    20461 tcgatgcccg tgtgaagctg aaaaagcagg gcaagaattt ccacgcgtgt tgtccattcc
    20521 acaacgagaa aaccccgtca ttcaccgtta acggtgagaa acagttttac cactgctttg
    20581 gatgtggcgc gcacggcaac gcgatcgact tcctgatgaa ctacgacaag ctcgagttcg
    20641 ttgaaacggt cgaagagctg gcagcaatgc acaatcttga agtgccattt gaagcaggca
    20701 gcggccccag ccagatcgag cgccatcaga ggcaaacgct ttatcagttg atggacggtc
    20761 tgaatacgtt ttaccaacaa tctttacaac aacctgttgc cacgtctgcg cgccagtatc
    20821 tggaaaaacg cggattaagc cacgaggtta tcgctcgctt tgcgattggt tttgcgcccc
    20881 ccggctggga caacgtcctg aagcggtttg gcggcaatcc agaaaatcgc cagtcattga
    20941 ttgatgcggg gatgttggtc actaacgatc agggacgcag ttacgatcgt ttccgcgagc
    21001 gggtgatgtt ccccattcgc gataaacgcg gtcgggtgat tggttttggc gggcgcgtgc
    21061 tgggcaacga tacccccaaa tacctgaact cgccggaaac agacattttc cataaaggcc
    21121 gccagcttta cggtctttat gaagcgcagc aggataacgc tgaacccaat cgtctgcttg
    21181 tggtcgaagg ctatatggac gtggtggcgc tggcgcaata cggcattaat tacgccgttg
    21241 cgtcgttagg tacgtcaacc accgccgatc acatacaact gttgttccgc gcgaccaaca
    21301 atgtcatttg ctgttatgac ggcgaccgtg caggccgcga tgccgcatgg cgagcgctgg
    21361 aaacggcgct gccttacatg acagacggcc gtcagctacg ctttatgttt ttgccggatg
    21421 gcgaagaccc tgacacgctg gtacgaaaag aaggtaaaga agcgtttgaa gcgcggatgg
    21481 agcaggcgat gccactctcc gcatttctgt ttaacagcct gatgccgcaa gttgatctga
    21541 gtacccctga cgggcgcgca cgtttgagta cgctggcact gccattgata tcgcaagtgc
    21601 cgggcgaaac gctgcgaata tatcttcgtc aggaattagg caacaaatta ggcatacttg
    21661 atgacagcca gcttgaacga ttaatgccaa aagcggcaga gagcggcgtt tctcgccctg
    21721 ttccgcagct aaaacgcacg accatgcgta tacttatagg gttgctggtg caaaatccag
    21781 aattagcgac gttggtcccg ccgcttgaga atctggatga aaataagctc cctggacttg
    21841 gcttattcag agaactggtc aacacttgtc tctcccagcc aggtctgacc accgggcaac
    21901 ttttagagca ctatcgtggt acaaataatg ctgccaccct tgaaaaactg tcgatgtggg
    21961 acgatatagc agataagaat attgctgagc aaaccttcac cgactcactc aaccatatgt
    22021 ttgattcgct gcttgaactg cgccaggaag agttaatcgc tcgtgagcgc acgcatggtt
    22081 taagcaacga agaacgcctg gagctctgga cattaaacct ggagctggcg aaaaagtgat
    22141 ttaacggctt aagtgccgaa gagcgatcgg gaagcccccg acagccgcac tgagaggcag
    22201 cggcaaatat ataagtacgc cctcgtaatt atcgttggcg gtaaacaacc gttggatttc
    22261 agcgttaacg gctgaaggac atcgggtcaa tcgcccaaca ccaacctcat gaaataagtg
    22321 tggataccgt cttatggagc aaaacccgca gtcacagctg aaacttcttg tcacccgtgg
    22381 taaggagcaa ggctatctga cctatgccga ggtcaatgac catctgccgg aagatatcgt
    22441 cgattcagat cagatcgaag acatcatcca aatgatcaac gacatgggca ttcaggtgat
    22501 ggaagaagca ccggatgccg atgatctgat gctggctgaa aacaccgcgg acgaagatgc
    22561 tgccgaagcc gccgcgcagg tgctttccag cgtggaatct gaaatcgggc gcacgactga
    22621 cccggtacgc atgtacatgc gtgaaatggg caccgttgaa ctgttgaccc gcgaaggcga
    22681 aattgacatc gctaagcgta ttgaagacgg gatcaaccag gttcaatgct ccgttgctga
    22741 atatccggaa gcgatcacct atctgctgga acagtacgat cgtgttgaag cagaagaagc
    22801 gcgtctgtcc gatctgatca ccggctttgt tgacccgaac gcagaagaag atctggcacc
    22861 taccgccact cacgtcggtt ctgagctttc ccaggaagat ctggacgatg acgaagatga
    22921 agacgaagaa gatggcgatg acgacagcgc cgatgatgac aacagcatcg acccggaact
    22981 ggctcgcgaa aaatttgcgg aactacgcgc tcagtacgtt gtaacgcgtg acaccatcaa
    23041 agcgaaaggt cgcagtcacg ctgccgctca ggaagagatc ctgaaactgt ctgaagtatt
    23101 caaacagttc cgcctggtgc cgaagcagtt tgactacctg gtcaacagca tgcgcgtcat
    23161 gatggaccgc gttcgtacgc aagaacgtct gatcatgaag ctctgcgttg agcagtgcaa
    23221 aatgccgaag aaaaacttca ttaccctgtt taccggcaac gaaaccagcg atacctggtt
    23281 caacgcggca attgcgatga acaagccgtg gtcggaaaaa ctgcacgatg tctctgaaga
    23341 agtgcatcgc gccctgcaga aactgcagca gattgaagaa gaaaccggcc tgaccatcga
    23401 gcaggttaaa gatatcaacc gtcgtatgtc catcggtgaa gcgaaagccc gccgtgcgaa
    23461 gaaagagatg gttgaagcga acttacgtct ggttatttct atcgccaaga aatacaccaa
    23521 ccgtggcttg cagttccttg acctgattca ggaaggcaac atcggtctga tgaaagcggt
    23581 tgataaattc gaataccgcc gtggttacaa gttctccacc tacgcaacct ggtggatccg
    23641 tcaggcgatc acccgttcta tcgcggatca ggcgcgcacc atccgtattc cggtgcatat
    23701 gattgagact atcaacaaac tcaaccgtat ttctcgccag atgctgcaag agatgggccg
    23761 tgaaccgacg ccggaagaac tggctgaacg tatgctgatg ccggaagaca agatccgcaa
    23821 agtgctgaag atcgccaaag agccaatctc catggaaacg ccgatcggtg atgatgaaga
    23881 ttcgcatctg ggggatttca tcgaggatac caccctcgag ctgccgctgg attctgcgac
    23941 caccgaaagc ctgcgtgcgg caacgcacga cgtgctggct ggcctgaccg cgcgtgaagc
    24001 gaaagttctg cgtatgcgtt tcggtatcga tatgaacacc gaccacacgc tggaagaagt
    24061 gggtaaacag ttcgacgtta cccgcgaacg tatccgtcag atcgaagcga aggcgctgcg
    24121 caaactgcgt cacccgagcc gttctgaagt gttgcgtagc ttcctggacg attaatcggt
    24181 aggccggatc aggcgttacg ccgcacccgg cactagtccc tttgcacaaa cgccaccttt
    24241 tcggtggcgt tttttatcgc ccacgcacta ccagcgcctg gtccagctcg cgatatgctt
    24301 caaccagttt ctctagtgaa acgcgactta aaccgctggg atttggcagc acccaaatct
    24361 gcgtcgaacc aatggtgagc gtttgtttcc cccactgtgc accgcgctgg ctgaatccct
    24421 gttcatatgc ttgtttgccc agaatcgcca acgcctgcgg ctgataatct tcaatttttt
    24481 caatcagctt acgcccacct gcgtgcagct cctgctttga aacttcattg gcttgcaccg
    24541 ttggacggtc taccagtttg gtgacgccac aacgataatc cagcagatgc tgtgcctcct
    24601 gcggcttcaa ctgacggtcg gtaaacccgg cctgatatat caccttccag aagcgatttg
    24661 ctggatgagc aaagggaaaa ccagtcccgg cggatgaaag cccagggttg ataccgcaaa
    24721 acacgacccg taaccctaga gccaaaatat cctcaaccat atttactcct ttctaacatc
    24781 accaggaaaa gtataacgga ttgaaaatac attgtttata aaaacagcag gcgcacggta
    24841 atggctggat tgcgacacgg agttacttta taatccgcta ccatggcccc ttagctcagt
    24901 ggttagagca ggcgactcat aatcgcttgg tcgctggttc aagtccagca ggggccacca
    24961 gatatagcaa aggctgacga gaaatcgtca gcctttttct ttttatatat cagttacttt
    25021 gcgtgccagt aagccgctgc acgtacccgc tgtgggtcat actgttccgc ttcaaagcgg
    25081 cggcttaaat tcttaacgac tttaccttcg ccggttatcc agatgaagta atcatcggca
    25141 gggatttgca tctgcgccag acgcgcatct accgcctgct catcatgtgc cagccattcg
    25201 atattaaaac catcaaggtg cgcgagataa tcctgacagg cgttatcccg cacgctaacc
    25261 agcgcactaa cttgcggttt aacggcaagt ttgctcaacg tttccaggcg gcggcgcagt
    25321 gcgggcattc cggattcatc gcagacatac agctgatacg cgtaatcttc cggcaccacc
    25381 agcgaaccgc gcggacctgc caccgtaagt ttatcgcccg gttgcgcctg catcgcccag
    25441 ccgctggcga ccccaccgtc gtgaataaag aaatcgatcg ccagttcatg gcgtagttcg
    25501 tcatacagcg gcgtatagtc acgcgacagt gggcgtggtc cttccggcca gacgatgccc
    25561 tcttccgtta ccgttggcgg cacaaagtga gcgtcaggtt gaggaaagaa gagtttgctg
    25621 tgatcgtcaa agccgcgcga tgtaaaaccg tccagcgcct cgccgccgag gacaatgcgc
    25681 tgaaaaccgg cgctgatgcg ctcaacgcgt aacacagtca gttcacggaa gcgcagatca
    25741 ttgcgaacgc gctgcgggta gcggggggag ttattcattg ttatcgcctt cgtgatagta
    25801 atcagatata tctaaatgat tttcgtaaat gataatgatt gttaatcatg ataaatgcaa
    25861 gcgatttgta gaactgatat atctgaagta tgtgaaggca ttcagtgcca tagcaatgct
    25921 gccggatgcg gcgtgaacgt cttatccggc ctggatgtga ggtgcttcca cgtttaaaaa
    25981 tcatttttgt acttgcagta atgctcacat cagatataaa ttagatatat ctaatcaagc
    26041 aaaaggaggc tgatatgagc catcatcacg aagggtgttg taaacatgaa ggccagccac
    26101 gccatgaggg ctgctgcaaa ggtgagaagt cagaacacga gcactgcgga cacggtcacc
    26161 agcatgaaca cggtcaatgc tgcggtggtc gccacggtcg cggcggcggt cgtcggcaac
    26221 gtttctttgg tcacggtgaa ttgcgtctgg tgattctgga tattctctcg cgcgatgaca
    26281 gccacggtta cgaattgatt aaagcgattg agaacctaac ccaggggaat tacaccccaa
    26341 gcccgggcgt catctaccca acgctggatt ttctgcagga gcagtcgctg attaccatcc
    26401 gcgaagagga aggtggtaag aagcagattg cgctgaccga acaaggcgcg cagtggctgg
    26461 aagaaaaccg cgaacaggtg gagatgattg aagaacgcat caaagcgcgt tgcgttggcg
    26521 cggcgctgcg ccagaacccg caaatgaagc gggcgctgga taattttaaa gcggtgctgg
    26581 atttacacgt caaccagagc gatatcagtg atgcacaaat aaaaaagatc attgcggtga
    26641 tcgaccgcgc cgcttttgat attacgcaac tggattaatc gccgcatccg ccagtggcgc
    26701 ggtgcaattg ccggatgcga cgcttgacgc gccttatccg gcctacaccc gctacacacc
    26761 ccgcaggcct gataagatgc gccagcatcg catcaggcat tgtgctccaa ccgccggatc
    26821 cggcataccg attaatgcag caccgtcacc gcgtcttcca gtcggctggc gcggtgtttc
    26881 accatcgccg acacctgcgc actctcttcc accagctcgg catttttctg ggtgatcagg
    26941 ttaagctcat ccactgcacg ggtcaggctg gaaagcccat cggcctgttc cagcgttgaa
    27001 tggctaatct gggcgatcaa ctgggtgacg tttttcacct gtgccacaat atcttccatc
    27061 gtccgtccgg cggcgtgtac ctgctgcgaa ccggattgca ccttatcagc actggcatca
    27121 atcagcttgc gaatatcgtt ggcagcatta gcgctgcggc tggctaaatg acgcacttcc
    27181 cctgccacca ctgcaaaacc tttgccctgt tcgccggcac gcgccgcttc caccgccgca
    27241 ttcagggcca gaatattggt ctgaaacgca atatcgttaa tcagcgaagt aatggtgcca
    27301 atgcgctggg tactgtcggc gatatcgtcc attgtcttga tcaccgtggt catcgcctcc
    27361 ccaccctgca ctgccgcatt actggcggtg atagaaagtt tatcggcagc cgacgccgtg
    27421 gcagagttct gtttcaccga cgccgccatt tggttcatgg tcgccaccgt ttgctgaacg
    27481 ttatcaactg tctgctgggt atgttcgttc agttcatcag tgcctttcgc cagcgtctca
    27541 ctgccatttc tgacactgga cacctggctt gagacatcgt taattagcca acggcacatc
    27601 aggccaagct gccctaccgc gcgtaatgtc agccccagct catcgctgcg attcaggtgc
    27661 tcaacactat tacgctctcc ggtcgccacc ttcagcgcct gacgggcgac attttctatc
    27721 gggcgcacaa tctgccattc aaaacaagcg cttgccaaca ataccactaa cacacagagg
    27781 aaatacgtca ccaccggggc agcaacaaac caaagcatgg ccgccagcaa gataaacatc
    27841 agggtcatca ctccacgcgc ccgccagcga agcggtaatg aaggcagttt acccagccag
    27901 cctttacgca ccaccaggcc tttatgaata cgcttactgg tacgtccggc gttcagcgct
    27961 ttgtacagcg gctccaccgc cgcaatctct tcatccgtcg cccgggtacg aatcgacata
    28021 tagccactga tttttccctc gcgcaccatc ggtaccgcat tggcccgcac ccaataatgg
    28081 tcgccatttt tgcggcgatt tttcacgatg ccgctccagg gctccccttt tttcagggtg
    28141 aaccacatat ccgcaaacgc cgcttttggc atatccgggt gacgcaccat gttgtgcggc
    28201 tgcccttgta actcttgcaa ggtaaagccg ctcacctgca caaaagtgtc attagcatga
    28261 gtgatatagc tttgcagatc ggtagtggac atcagagtgg tatcgtccgc cagcggggta
    28321 ttttgctggg tgacatacgg atgagaagac atgttcgcgt cctgtgcagg ttatatggtt
    28381 gttaacttct tgtcagagtt tatgtcggcc ccgctgcggt tatctttaac cgattaattt
    28441 gatttagatc gcaatttgcg attaaagcac aaatctaatt ctttgattta aaatactttc
    28501 actctgttac tatacgaaaa cgttaattat cttgcccaaa aatcaggcaa ttattgccct
    28561 gaaaacgtgc atttgcgcag caatcatcaa atccataccc gacaaaaacc gtgcaaaata
    28621 acaacaaatg ttaacagata gcattaaata ttgcacaaat gataaccgaa tttgtgttta
    28681 tcccgatttt cgcgatcgca gccggagtgg cgcaatccct gcaatactta aatcggtatc
    28741 atgtgatacg cgagcctccg gagcatattt tgaacaggtt accttcgagc gcatcggctt
    28801 tagcgtgcag cgcccacgcc ctgaatctca ttgagaagcg aacgctggat catgaggaga
    28861 tgaaagcact taaccgagag gtgattgaat acttcaaaga gcatgtcaat ccggggtttt
    28921 tagagtatcg caaatctgtt accgccggcg gggattacgg agccgtagag tggcaagcgg
    28981 gaggtttaaa tacgcttgtc gacacccagg gacaggagtt tatcgactgc ctgggaggtt
    29041 ttggaatttt caacgtgggg caccgtaatc cagttgtggt ttccgccgta cagaatcaac
    29101 ttgcgaaaca accgctgcac agccaggagc tgctcgatcc gttacgggcg atgttggcga
    29161 aaacccttgc tgcgctaacg cccggtaaac tgaaatacag cttcttctgt aatagcggca
    29221 ccgagtccgt tgaagcagcg ctgaagctgg cgaaagctta ccagtcaccg cgcggcaagt
    29281 ttacttttat tgccaccagc ggcgcgttcc acggtaaatc acttggcgcg ctgtcggcca
    29341 cggcgaaatc gaccttccgc aaaccgttta tgccactgct gccgggcttc cgtcatgtgc
    29401 cgtttggcaa tatcgaagcc atgcgcacgg ctcttaacga gtgcaaaaaa accggtgatg
    29461 atgtggctgc ggtgatcctc gaaccgattc agggtgaagg tggcgtaatt ctgccgccgc
    29521 cgggctatct caccgccgta cgtaagctat gcgatgagtt cggcgcactg atgatcctcg
    29581 atgaagtaca aacgggcatg gggcgcacgg gcaagatgtt cgcctgcgag catgagaacg
    29641 tacagccgga tatcctctgc cttgccaaag cgctcggcgg cggcgtgatg ccgattggcg
    29701 cgaccatcgc cactgaagag gtgttttcag ttctgttcga caacccattc ctgcatacca
    29761 ccacctttgg cggcaacccg ctggcctgtg cggcggcgct ggcgaccatc aatgtgttgc
    29821 tggagcagaa cttaccggct caggctgagc aaaaaggcga tatgttgctg gacggtttcc
    29881 gtcaactggc gcgggaatat cccgatctgg tacaggaagc gcgtggtaaa gggatgttga
    29941 tggcaattga gtttgttgat aacgaaatcg gctataactt tgccagcgag atgttccgcc
    30001 agcgcgtact ggtggccgga acgctcaata acgccaaaac gatccgcatt gaaccgccac
    30061 tgacactgac cattgaacag tgtgaactgg tgatcaaagc ggcgcgtaag gcgctggcgg
    30121 ccatgcgagt aagtgtcgaa gaagcgtaat atcaaatcgg atggcgatac gacgtcgcca
    30181 tccgatcttt ttatacgacg cgtacgcccg caggcatcat ccgctccggc gttaacaaca
    30241 cgctttcact gccgtcatcg gtttccgcac acaacagcat gcattccgac gtttcaccac
    30301 gcattttcgc tttttgcaga ttgcataaga ccaccaccgt tttccccatc agctcttctt
    30361 cgctgtagta cggcaccagg ctggtcacgg tttgcagtgt tttttccccc acatcaacct
    30421 gtacgatgta cagcttgtcg gcgttttcat ggcgtttcac ttccacaatc tttccgacgc
    30481 gcatttccag acgtgcaaaa tcagcgtaag ccacggtttc cattgtttcc tcccttcggg
    30541 taaaatttca ctaaactata gcaaaagttt ttctcaatcc tgtaggctaa aaatggagaa
    30601 tgcaggcgtg atcacattcg tgagccgctg tgttaccgtt acagcgtcaa agaaacgcgc
    30661 tttatttact gaaaacaggt gacccgataa gcacttcctc tacaatgggg gcgcacatca
    30721 gggaaagtaa aaaaggtaaa catggcaaca ctaaaagaca tcgcaatcga agctggcgta
    30781 tccctggcga cagtatccag ggtcttaaat gacgatccga cattgaatgt gaaagaagag
    30841 acgaaacatc gcattctcga gatcgccgaa aagctggagt acaagaccag tagtgcccgt
    30901 aaactccaga caggtgcagt caaccaacac catattctgg ctatctacag ctaccagcag
    30961 gagctggaga tcaacgatcc ttactatctg gcgatccgcc acggcattga aacccagtgc
    31021 gaaaagctgg gcatcgagct caccaactgt tatgaacaca gcggcttacc agacattaaa
    31081 aacgtcaccg gtattttaat tgtcggcaaa cccacgcccg ccctgcgcgc cgctgccagc
    31141 gcgttgaccg acaatatctg ttttatcgac tttcacgaac ccggcagcgg ttacgatgcg
    31201 gtggatatcg atctggcacg catcagtaaa gaaatcatcg acttctatat caaccagggc
    31261 gttaatcgta ttggttttat tggcggtgaa gatgagcctg gcaaggcgga tattcgtgag
    31321 gtcgcctttg cggaatatgg ccgactgaaa caagtggtac gcgaagagga tatctggcgc
    31381 ggcggttttt ccagttcgtc gggttatgaa ctggcaaaac aaatgctggc gcgggaagac
    31441 tatccgaagg cactgtttgt tgcttccgat tccattgcta tcggcgtact gcgggcaatt
    31501 catgaacgag gcctgaacat cccacaggat atttcgctta tcagcgttaa cgatatcccc
    31561 accgcgcgat ttacctttcc gccgctctcc accgtgcgca tccattccga gatgatggga
    31621 agtcagggcg ttaacctggt gtatgaaaaa gcccgcgatg gtcgcgcgct gccgctgtta
    31681 gtcttcgttc ccagcaaatt aaaactgcgc ggcacgaccc gttaaatccc cttacacact
    31741 gtccggcaat cgtttttgcc ggacagtgct gccgtttatt ttcgtgatcc agttaaagta
    31801 aatgcattta cctgctactt tttagtaaaa attttactaa actccccagc aattacacaa
    31861 actaccatca ccatgaatgg ttccgatttc tctctaccgg gaggccctat gaatcgctgg
    31921 gaaaacattc agctcaccca cgaaaaccga cttgcgccgc gtgcgtactt tttttcatat
    31981 gattctgttg cgcaagcgcg tacctttgcc cgcgaaacca gcagcctgtt tctgccctta
    32041 agcggtcagt ggaatttcca cttttttgac catccgctgc aagtaccaga agccttcacc
    32101 tctgagttaa tggctgactg ggggcatatt accgtccccg ccatgtggca aatggaaggt
    32161 cacggcaaac tgcaatatac cgacgaaggt tttccgttcc ccatcgatgt gccgtttgtc
    32221 cccagcgata acccaaccgg tgcctatcaa cgtattttca ccctcagcga cggctggcag
    32281 ggtaaacaga cgctgattaa atttgacggc gtcgaaacct attttgaagt ctatgttaac
    32341 ggtcagtatg tgggtttcag caagggcagt cgcctgaccg cagagtttga catcagcgcg
    32401 atggttaaaa ccggcgacaa cctgttgtgt gtgcgcgtga tgcagtgggc ggactctacc
    32461 tacgtggaag accaggatat gtggtggtca gcggggatct tccgcgatgt ttatctggtc
    32521 ggaaaacacc taacgcatat taacgatttc actgtgcgta ccgactttga cgaagcctat
    32581 tgcgatgcca cgctttcctg cgaagtggtg ctggaaaatc tcgccgcctc ccctgtcgtc
    32641 acgacgctgg aatatacctt gtttgatggc gaacgcgtgg tgcacagcag cgccattgat
    32701 catttggcaa ttgaaaaact gaccagcgcc agctttgctt ttactgtcga acagccgcag
    32761 caatggtcag cagaatcccc ttatctttac catctggtca tgacgctgaa agacgccaac
    32821 ggcaacgttc tggaagtggt gccacaacgc gttggcttcc gtgatatcaa agtgcgcgac
    32881 ggtctgttct ggatcaataa ccgttatgtg atgctgcacg gcgtcaaccg tcacgacaac
    32941 gatcatcgca aaggccgcgc cgttggaatg gatcgcgtcg agaaagatct ccagttgatg
    33001 aagcagcaca atatcaactc cgtgcgtacc gctcactacc cgaacgatcc gcgtttttac
    33061 gaactgtgtg atatctacgg cttgtttgtg atggcggaaa ccgacgtcga atcgcacggc
    33121 tttgctaatg tcggcgatat cagccgtatt accgacgatc cgcagtggga aaaagtctac
    33181 gtcgagcgca ttgttcgcca tattcacgcg cagaaaaacc atccgtcgat catcatctgg
    33241 tcgctgggca atgaatccgg ctatggctgt aacatccgcg cgatgtacca cgcagcgaag
    33301 gcgctggatg acacgcgact ggtgcattac gaagaagatc gcgatgctga agtggtcgat
    33361 attatttcca ccatgtacac ccgcgtgccg ctgatgaatg agtttggtga atacccgcat
    33421 ccgaagccgc gcatcatctg tgaatatgct catgcgatgg ggaacggacc aggcgggctg
    33481 acggagtacc agaacgtctt ctataagcac gattgtattc agggacatta tgtttgggag
    33541 tggtgcgacc acgggatcca ggcgcaggat gacaacggca acgtctggta taaattcggc
    33601 ggcgactacg gcgactatcc caacaactat aacttctgtc ttgatggttt gatctattcc
    33661 gatcagacgc cgggaccggg cctgaaagag tacaaacagg ttatcgcgcc ggtaaaaatc
    33721 cacgcgctgg atctgactcg cggcgagctg aaagtcgaaa ataaactgtg gtttaccacg
    33781 cttgatgact acaccctgca cgcagaggtg cgcgtcgaag gtgaaacgct cgcgacgcag
    33841 cagattaaac tgcgcgacgt tgcgccgaac agcgaagccc ccttgcagat cacgctgccg
    33901 cagctggacg cccgcgaagc gttcctcaac attacggtga ccaaagattc ccgcacccgc
    33961 tacagcgaag ccgggcattc tatcgccact tatcagttcc cgctgaagga aaacaccgcg
    34021 caaccagtgc ctttcgcacc aaataatgcg cgtccgctga cgctggaaga cgatcgtttg
    34081 agctgcaccg ttcgcggcta caacttcgcg atcaccttct caaaaatgag tggcaaaccg
    34141 acatcctggc aggtgaatgg cgagtcgctg ctgacccgcg agccaaagat caacttcttc
    34201 aagccaatga tcgacaacca caagcaggag aacgaagggc tgtggcaacc gaatcatttg
    34261 cagatcatgc aggaacatct gcgcgacttt gccgtagaac agagcgatgg tgaagtgttg
    34321 atcatcagcc gcacagttat tgccccgccg gtgtttgact tcgggatgcg ctgcacctac
    34381 atctggcgca tcgctgccga tggccaggtt aacgtggcgc tttccggcga gcgttacggc
    34441 gactatccgc acatcattcc gtgcatcggt ttcaccatgg ggattaacgg cgaatacgat
    34501 caggtggcgt attacggtcg tggaccgggc gaaaactacg ccgacagcca gcaggctaac
    34561 atcatcgata tctggcgcag caccgtcgat gccatgttcg agaactatcc cttcccgcag
    34621 aacaacggca accgtcagca tgtccgctgg acggcactga ctaaccgcca cggcaacggt
    34681 ctgctggtgg ttccgcagcg cccaattaac ttcagcgcct ggcactatac ccaggaaaac
    34741 atccacgctg cccagcactg taacgagctg cagcgcagtg atgacatcac cctgaacctc
    34801 gaccaccagc tacttggcct cggctccaac tcctggggca gcgaggtgct ggactcctgg
    34861 cgcgtctggt tccgtgactt cagctacggc tttacgttgc tgccggtttc tggcggagaa
    34921 gctaccgcgc aaagcctggc gtcgtatgag ttcggcgcag ggttcttttc cacgaatttg
    34981 cacagcgaga ataagcaatg aggatcatcg ataacttaga acagttccgc cagatttacg
    35041 cctctggcaa gaagtggcaa cgctgcgttg aagcgattga aaatatcgac aacattcagc
    35101 ctggcgtcgc ccactccatc ggtgactcat tgacttaccg cgtggagaca gactccgcga
    35161 ccgatgcgct atttaccggg catcgacgct attttgaagt gcattactac ctgcaagggc
    35221 agcaaaaaat tgaatatgca ccgaaagaga cattacaggt agtggaatat tatcgtgatg
    35281 aaactgaccg tgaatattta aaaggctgcg gagaaaccgt tgaggtccac gaagggcaaa
    35341 tcgttatttg cgatatccat gaagcgtatc ggtttatctg caataacgcg gtcaaaaaag
    35401 tggttctcaa agtcaccatc gaagatggtt atttccataa caaataacaa ctacggcggc
    35461 aaaaggagtt tgccgccacc gctaccctac acattttcgg agatgtgtta tgtctgatac
    35521 caaacgtaat acaatcggca aattcggctt gctctcgctg acttttgccg ccgtttacag
    35581 ctttaacaac gttatcaata ataatattga gcttggactg gcctcggcac cgatgttttt
    35641 cctcgcgacg attttttatt ttattccctt ctgtctgatc atcgcagaat ttgtttcgtt
    35701 aaataaaaac tcagaagccg gtgtctacgc gtgggtaaaa agttcgctgg gcggacgttg
    35761 ggcatttatt actgcctata cctactggtt cgtaaacctg ttctttttca cctcactgtt
    35821 gccgcgcgtt attgcttatg cttcgtatgc cttcctcgga tacgaatata ttatgacgcc
    35881 ggttgccacc accattatca gtatggtgct gttcgccttc tccacctggg tttccaccaa
    35941 cggggcgaaa atgttggggc caattacctc cgtcacttca acgctgatgc tgctgttaac
    36001 gctctcctac attttactgg caggtacggc gctggttggc ggcgtacagc ctgctgaccc
    36061 catcaccgtt gacgcgatga tcccgaactt caactgggcg ttcctcggcg ttaccacctg
    36121 gatctttatg gccgcaggtg gcgcggagtc cgtcgctgtg tacgttaacg acgtcaaagg
    36181 cggttcgaaa tcgttcgtta aagtgatcat cctcgccggg atttttatcg gcgtactgta
    36241 ttccgtctcc tcggtgctga ttaacgtctt cgtcagcagc aaagagttga aatttaccgt
    36301 cggatcggtg caggtattcc acggcatggc ggcgtatttt ggtctaccgg aagcgttgat
    36361 gaatcgcttt gtcggtctgg tgtcctttac cgcgatgttc ggttccctgc tgatgtggac
    36421 cgcaacgccg gtgaaaattt tcttctccga aatcccggaa ggcatctttg gtaagaaaac
    36481 cgtcgaactg aacgaaaacg gcgttccggc gcgcgcagcg tggatccagt tcctgatcgt
    36541 catcccgctg atgattatcc cgatgctcgg ttccaatacc gtgcaggatc tgatgaatac
    36601 tattattaat atgaccgccg cagcgtccat gcttccgccg ttattcatca tgctggctta
    36661 cctgaattta cgcgccaaat tagatcacct gccacgcgat ttccgtatgg gctcccgccg
    36721 caccggtatt atcgttgttt caatgctgat tgcgatattt gccgtagggt ttgtcgcttc
    36781 gacattcccg actggcgcga atattctgac catcattttt tataacgtcg gcggtattgt
    36841 tatcttcctt ggctttgcgt ggtggaaata cagtaaatat ataaagggat taacggctga
    36901 agagcgccat attgaagcga cgccagccag caatgttgat taagcagaat aataaaaaat
    36961 ga
//
LOCUS       DAZGJW010000030        36941 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZGJW010000030 DAZGJW010000000
VERSION     DAZGJW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.030"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..316)
                     /locus_tag="ACHKIK_001585"
     CDS             complement(<1..316)
                     /locus_tag="ACHKIK_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9082782.1"
                     /translation="MENVRFLQGVKQYFCTKALDVILLIYLFLGFILLPYKYLWKDII
                     LSLCFVGILLYALVEENRITEYMGYFVKFSNKNRLNSCAAWCALIACFIFLFLVLSIN
                     IFS"
     gene            457..2889
                     /gene="hsdR"
                     /locus_tag="ACHKIK_001586"
     CDS             457..2889
                     /gene="hsdR"
                     /locus_tag="ACHKIK_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFO9082783.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKAYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2956..4425
                     /locus_tag="ACHKIK_001587"
     CDS             2956..4425
                     /locus_tag="ACHKIK_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO9082784.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     VLELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMKTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRIENEQAWKVSIDDVIARNFNLDIKNPHQAETI
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4425..6194
                     /locus_tag="ACHKIK_001588"
     CDS             4425..6194
                     /locus_tag="ACHKIK_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO9082785.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWTRLINLGIWALGSGFPNVVQGSTDKEILMCKVSDMNLEGNEKFIFSTKNTISK
                     DLADEYKIKISEPGTIIFPKIGGAIATNKRRILVQDTAIDNNCLGIKPCDAISGEWFY
                     LILNTLDMSKYQSGTSIPAINQSVIGSIPIALPSLKMQEKIVSYVITLMSLCDQLEQQ
                     SLTSLDAHQQLVETLLGTLTDSQNAEELAENWARISEHFDTLFTTEASVDALKQTILQ
                     LAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEG
                     WEWCKFGLTSEFINGDRGSNYPNKNEYVSQGIPWINTGHIEKNGTLTVTEMNFITEGK
                     FNELRSGKIQKGDLVYCLRGATFGKTAFVIPYETGAIASSLMIIRPFITEMGGYIYNY
                     LTSPFGRSQIYRFDNGSAQPNLSANSVMLYSFPCPPLTEQYRIFSQVGLLHELCDKLK
                     TRIKTAQQTQLHLADALTDAAIN"
     gene            6415..6756
                     /gene="symE"
                     /locus_tag="ACHKIK_001589"
     CDS             6415..6756
                     /gene="symE"
                     /locus_tag="ACHKIK_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO9082786.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPTAEESELMQSLRQVCKLSARKQKQVQDF
                     IGVITGKQKVA"
     gene            complement(6803..7966)
                     /locus_tag="ACHKIK_001590"
     CDS             complement(6803..7966)
                     /locus_tag="ACHKIK_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFO9082787.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFMNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRVHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIRARADRLADLAVQVWQAPSLSEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(8014..8896)
                     /locus_tag="ACHKIK_001591"
                     /pseudo
     CDS             complement(8014..8896)
                     /locus_tag="ACHKIK_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(8993..9157)
                     /gene="yjiS"
                     /locus_tag="ACHKIK_001592"
     CDS             complement(8993..9157)
                     /gene="yjiS"
                     /locus_tag="ACHKIK_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO9082788.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            9334..10746
                     /gene="yjiR"
                     /locus_tag="ACHKIK_001593"
     CDS             9334..10746
                     /gene="yjiR"
                     /locus_tag="ACHKIK_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO9082789.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSREHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWGAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQNYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(10988..11932)
                     /locus_tag="ACHKIK_001594"
     CDS             complement(10988..11932)
                     /locus_tag="ACHKIK_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO9082790.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIYLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVNTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRALPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKKFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            12399..13631
                     /gene="mdtM"
                     /locus_tag="ACHKIK_001595"
     CDS             12399..13631
                     /gene="mdtM"
                     /locus_tag="ACHKIK_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO9082791.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVHQHQAAELAEER"
     gene            13672..14952
                     /gene="yjiN"
                     /locus_tag="ACHKIK_001596"
     CDS             13672..14952
                     /gene="yjiN"
                     /locus_tag="ACHKIK_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO9082792.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            15068..16219
                     /gene="yjiM"
                     /locus_tag="ACHKIK_001597"
     CDS             15068..16219
                     /gene="yjiM"
                     /locus_tag="ACHKIK_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO9082793.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            16229..16996
                     /gene="yjiL"
                     /locus_tag="ACHKIK_001598"
     CDS             16229..16996
                     /gene="yjiL"
                     /locus_tag="ACHKIK_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418754.2"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO9082794.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEAISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            16993..17250
                     /gene="ytiA"
                     /locus_tag="ACHKIK_001599"
     CDS             16993..17250
                     /gene="ytiA"
                     /locus_tag="ACHKIK_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO9082795.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEMQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            17315..18175
                     /gene="yjiK"
                     /locus_tag="ACHKIK_001600"
                     /pseudo
     CDS             17315..18175
                     /gene="yjiK"
                     /locus_tag="ACHKIK_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
     gene            18243..19421
                     /gene="yjiJ"
                     /locus_tag="ACHKIK_001601"
     CDS             18243..19421
                     /gene="yjiJ"
                     /locus_tag="ACHKIK_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9082796.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(19434..19988)
                     /gene="kptA"
                     /locus_tag="ACHKIK_001602"
     CDS             complement(19434..19988)
                     /gene="kptA"
                     /locus_tag="ACHKIK_001602"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO9082797.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRTLLDTVVATSDKKRFSYSSDGRCIRAVQGHLTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQTENGVWLTSTVAVEFLEW"
     gene            20238..20921
                     /gene="yjiH"
                     /locus_tag="ACHKIK_001603"
     CDS             20238..20921
                     /gene="yjiH"
                     /locus_tag="ACHKIK_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO9082798.1"
                     /translation="MTQQGDAVAGDLATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            20918..21379
                     /gene="yjiG"
                     /locus_tag="ACHKIK_001604"
     CDS             20918..21379
                     /gene="yjiG"
                     /locus_tag="ACHKIK_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO9082799.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            21392..22564
                     /gene="iadA"
                     /locus_tag="ACHKIK_001605"
     CDS             21392..22564
                     /gene="iadA"
                     /locus_tag="ACHKIK_001605"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO9082800.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILFPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            22629..23541
                     /gene="hypT"
                     /locus_tag="ACHKIK_001606"
                     /gene_synonym="qseD"
                     /pseudo
     CDS             22629..23541
                     /gene="hypT"
                     /locus_tag="ACHKIK_001606"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
     gene            complement(23534..23926)
                     /gene="iraD"
                     /locus_tag="ACHKIK_001607"
     CDS             complement(23534..23926)
                     /gene="iraD"
                     /locus_tag="ACHKIK_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO9082801.1"
                     /translation="MMRQSRQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSSLPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHTLAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(23923..24006)
                     /gene="idlP"
                     /locus_tag="ACHKIK_001608"
     CDS             complement(23923..24006)
                     /gene="idlP"
                     /locus_tag="ACHKIK_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO9082802.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(24122..24265)
                     /gene="ytiD"
                     /locus_tag="ACHKIK_001609"
     CDS             complement(24122..24265)
                     /gene="ytiD"
                     /locus_tag="ACHKIK_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFO9082803.1"
                     /translation="MADYAEINNFTPELSSGGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
     gene            24599..25429
                     /gene="yjiC"
                     /locus_tag="ACHKIK_001610"
     CDS             24599..25429
                     /gene="yjiC"
                     /locus_tag="ACHKIK_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO9082804.1"
                     /translation="MSMPLSNTLQSQIITDNHFLHHPKVDSELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVENYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(25570..26343)
                     /gene="uxuR"
                     /locus_tag="ACHKIK_001611"
     CDS             complement(25570..26343)
                     /gene="uxuR"
                     /locus_tag="ACHKIK_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO9082805.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDV"
     gene            complement(26558..28018)
                     /gene="uxuB"
                     /locus_tag="ACHKIK_001612"
     CDS             complement(26558..28018)
                     /gene="uxuB"
                     /locus_tag="ACHKIK_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO9082806.1"
                     /translation="MTTIVDSNLPVARPSWEHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKVNAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(28099..29283)
                     /gene="uxuA"
                     /locus_tag="ACHKIK_001613"
     CDS             complement(28099..29283)
                     /gene="uxuA"
                     /locus_tag="ACHKIK_001613"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO9082807.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            29735..29827
                     /locus_tag="ACHKIK_001614"
                     /pseudo
     CDS             29735..29827
                     /locus_tag="ACHKIK_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(30070..30972)
                     /gene="fimH"
                     /locus_tag="ACHKIK_001615"
     CDS             complement(30070..30972)
                     /gene="fimH"
                     /locus_tag="ACHKIK_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO9082808.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGSVLSNFSGTVKYSGSS
                     DPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTVDAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(30992..31495)
                     /gene="fimG"
                     /locus_tag="ACHKIK_001616"
     CDS             complement(30992..31495)
                     /gene="fimG"
                     /locus_tag="ACHKIK_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO9082809.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(31508..32038)
                     /gene="fimF"
                     /locus_tag="ACHKIK_001617"
     CDS             complement(31508..32038)
                     /gene="fimF"
                     /locus_tag="ACHKIK_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO9082810.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(32048..34684)
                     /gene="fimD"
                     /locus_tag="ACHKIK_001618"
     CDS             complement(32048..34684)
                     /gene="fimD"
                     /locus_tag="ACHKIK_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO9082811.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(34751..35476)
                     /gene="fimC"
                     /locus_tag="ACHKIK_001619"
     CDS             complement(34751..35476)
                     /gene="fimC"
                     /locus_tag="ACHKIK_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO9082812.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(35513..36052)
                     /gene="fimI"
                     /locus_tag="ACHKIK_001620"
     CDS             complement(35513..36052)
                     /gene="fimI"
                     /locus_tag="ACHKIK_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9082813.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(36117..36665)
                     /gene="fimA"
                     /locus_tag="ACHKIK_001621"
     CDS             complement(36117..36665)
                     /gene="fimA"
                     /locus_tag="ACHKIK_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO9082814.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         9333 a         9271 c         8893 g         9444 t
ORIGIN      
        1 ctgaaaaaat attaattgac aacactaaaa acaaaaatat aaaacacgca ataagtgcac
       61 accaggcagc acaactattc aacctgtttt tattactgaa cttaacaaaa taccccatat
      121 attctgtaat gcgattttct tccactaatg cataaagaag aataccaaca aagcacagag
      181 ataaaataat atctttccac agatatttat aagggagaag tataaaacca agaaatagat
      241 aaattagcag aatgacatct aatgccttag tacaaaaata ttgtttcact ccttgcaaga
      301 accgaacatt ctccatatga atccttatcc tactattgat gaaactcaag aataccttta
      361 aaacagtgac ttaacgctga taatactctt tgaacagatg aattcatcta tttttctgtg
      421 tagaatttgc gcatcgcatt tagatgagaa caccccatgg cggaactgaa cctaagtaac
      481 ctgacggaag cagacatcat taccaaatgc gttatgccag ccattctcaa tgcaggctgg
      541 gacaacacaa cgcaaatcag acaggaggtc aaactccggg acggtaaagt catcgtacgt
      601 ggtaaagttg cggcacgcag aacggtaaaa tctgctgaca tcgtgctgta tcacaaacca
      661 ggcattccct tagccgtgat tgaagcaaaa gccaacaaac atgaaattgg caaagggatg
      721 caacagggca ttgaatatgc gcgcctgctg gacgttcctt ttgtttttgc caccaacggg
      781 gatggcttta tcttccgcga tgccaccgca gccgaaggtg aatgccttga aaagcaaata
      841 acgctggatg acttcccctc ccccgctgaa ctctggcaaa aattctgcct ctggaaagga
      901 tacacacaag ctcagcttcc ggtgattact caggactatt acgacgatgg cagcggtaaa
      961 tcgccacgtt attaccagct tcaggcaatc aacaaaacca ttgaagccgt ctccaacggg
     1021 caaaaccgcg ttctgctggt catggcgacc ggaacaggga aaacctatac cgcattccag
     1081 atcatctggc gcctgtggaa atcaaaaaat aaaaaacgca ttttgttcct tgccgatcgc
     1141 aacattctgg tcgaccaaac caaaaataat gatttccagc catttggtac ggcaatgacc
     1201 aaagtcagcg gacgcaccat tgatcccgct tatgaaattc acctcgcgct ctatcaggct
     1261 ataactggcc cggaggaaga ccaaaaagcg tttaaacaag tcgcaccaga tttcttcgat
     1321 ctgatcgtga tcgacgaatg ccatcgcggc agcgcatccg aagacagcgc ctggcgagag
     1381 atccttgatt acttcagttc cgccacccaa attggcttaa ccgccacgcc aaaagagacg
     1441 catgaagtct ccagcacgga ttacttcggc gatccggttt acgtctactc gctaaaagaa
     1501 gggatcgaag acggcttcct cgccccttat aaagttgtcc gtgttgatat tgatgttgat
     1561 ctgcaaggct ggcgcccaac caaagggcaa actgacttaa acggcgaagt gatcgacgat
     1621 cgtatctata accagaaaga tttcgatcgc acgatggtaa tcgacgaacg gacagaactg
     1681 gttgccagaa ccattaccga ctacctcaag cgcaccaacc cgatggataa aaccatcgtc
     1741 ttctgtaacg acatcgatca tgcagaacgt atgcgccgcg ccctggtgaa tctcaacccg
     1801 gagcaggtga aaaagaacga caaatacgtc atgaaaatca ccggcgatga tgaaattggc
     1861 aaagctcagt tggataactt catcaacccg aaaaaagcgt acccggttat cgcgaccact
     1921 tcagagctga tgaccactgg tgtggatgct aaaacctgca aactggtagt actggaccag
     1981 aacatccagt cgatgaccaa attcaagcaa attatcggtc gtggtacacg catcgacgaa
     2041 cgttacggca aactctggtt taccatcctc gactttaaaa aagccaccga actgtttgcc
     2101 gatgagcgtt tcgatggcat tcccgaaaaa gtcatggata ccacaccaga ggatatcgcc
     2161 gatccagaat ctgattttga agagaaactc gaagaaatca gcgaacatga cgacgaacag
     2221 gtaacaggcg ttgatgaacc gcctgcgcca ccataccagg ttaccgatac cgatgatgtc
     2281 ggcccacttc cggaagaaga cgagaagaaa atccgcaagt ttcacgtcaa cggtgtagcg
     2341 gtgggcgtta ttgcgcagcg tgttcagtat tacgatgccg acggtaaact ggttaccgaa
     2401 tcttttaaag attacacccg caaaacactg ctcaaagaat atgcctcgct ggatgacttt
     2461 acccgcaagt ggcaggacgc cgatcgcaaa gaagcgatca ttcacgagct ggagcaacag
     2521 gggatcatct gggaagtact ggcagaagaa gtcggtaaag atctcgaccc gttcgacatg
     2581 ctttgccacg tagtgtatgg tcagccgccg ttaacccgca aagagcgcgc cgagaacgtg
     2641 cgcaagcgga actacttcac aaaatactct gaagcagcgc aagccgtgct cgataatctg
     2701 ctggataaat acgccgatgc gggcgtacag gagatcgaaa gtattcaggt gctgaaactt
     2761 aaaccattcg acagcatggg cactttaccg gagattatta aaaccggatt tggcgaccgt
     2821 aacgggtata atcaggcgct cagcgagctg gaaaacgaaa tctaccaatt accgccccgc
     2881 tctgcttaac aactctcttt tgtgccttct gcaaccaaca tgttgcagaa ggccttgttt
     2941 ttcatggaat acaacatgtc tatcagttca gtaatcaaat cattacaaga tattatgcgc
     3001 aaagatgccg gtgtggatgg cgatgcgcag cgtctcggtc agctctcctg gctgctgttt
     3061 ttgaaaatct tcgatgccca ggaagaggtg ctggaactgg agcaggataa ctatcaatat
     3121 ccgatcccac agcgttattt atggcgcagt tgggccgcaa acgctcaggg cattaccggt
     3181 gattccctgc tggaattcgt taatgatgat ctgttcccgg cgttgaaaaa cctcactgcg
     3241 cctatcgata aaaacccacg cggctacgtg gtgaagcagg cgttcagcga tgcctataac
     3301 tatatgaaaa acggtacgct actgcgccag gtgatcaaca agctgaacga aattgacttt
     3361 accagcgcca gcgaacgcca tctgtttggt gatatttacg aacagatcct taaagatctg
     3421 caatctgcgg gcaatgctgg cgaattctat acgccacgcg ccgtcactcg ctttatggtg
     3481 gatcgcgttg atccgaaact cggcgaatcc attatggacc cggcctgcgg tacgggcggt
     3541 tttcttgcct gcgcatttga tcatgtaaag aacaaatacg tgaagagcgt cgccgatcat
     3601 cagacgctgc aacaacagat ccacggtgtt gagaaaaaac agcttccgca cctgctggcg
     3661 accaccaata tgctgctgca cggcattgaa gtaccggtac aaattcgtca cgacaacacc
     3721 ctgaacaaac cgctttcctc ctgggatgaa caactggatg tcattgttac caacccgccg
     3781 tttggtggca cggaagaaga cggtattgag aagaactttc cggcagagat gaaaacccgc
     3841 gaaacggcgg atctgttcct gcaactgatt gtggaagtac tggcgaaaaa cggtcgcgcg
     3901 gcggtggtat tgccggatgg cacactattt ggcgaaggcg ttaaaaccaa aatcaaaaaa
     3961 ctgcttaccg aagagtgcaa cctgcatacc atcgtgcgtt taccgaatgg ggtgtttaac
     4021 ccctataccg gcattaaaac caacctgttg ttctttacca aaggtcagcc aaccaaagag
     4081 atttggttct atgagcatcc gtatccggcg ggcgtaaaaa actacagcaa aaccaagccg
     4141 atgaagtttg aagagtttca ggcggagatc gactggtggg gtaacgaggc cgatggtttt
     4201 gccagccgca tcgagaatga gcaagcgtgg aaagtcagca ttgatgacgt gattgcgcgt
     4261 aacttcaatc tggatattaa aaacccacat caggcggaaa ccattagcca tgatccggac
     4321 gaactgttag cgcagtatgc aaaacagcag gcggagatcc agacgctgcg taatcaactg
     4381 cgcgatattc ttggcgctgc gctgtctggc aaggaggtta actaatgagt gtggaaaagc
     4441 tgatcgttga tcatatggaa acctggacct cggcgttgca aacccgttcc accgccgggc
     4501 gcggcagctc cggtaagatt gatttgtatg gtattaagaa attgcgtgag ctgattctgg
     4561 aactggcggt gcgaggtaaa ctggtgccgc aggatccgaa cgatgaaccg gcgtcggagc
     4621 tgctgaagcg tattgcggcg gaaaaagcag agctggtgaa gcaaggtaaa attaaaaagc
     4681 aaaaaccgct gccggaaatt agcgaggaag agaagccgtt tgaattgccg gaggggtggg
     4741 aatggactcg attaattaat cttggtatat gggctttagg gtctggtttt ccaaatgtgg
     4801 tacagggaag cactgataaa gaaattttga tgtgcaaagt tagcgacatg aatcttgaag
     4861 gtaacgagaa attcattttt tcgacaaaaa atacaatttc aaaagaccta gctgatgaat
     4921 ataaaattaa aatctcagaa cccggcacca tcattttccc taaaattggc ggagcaattg
     4981 caactaataa acgaagaatt ttagttcagg atacagctat tgataacaac tgtcttggaa
     5041 taaaaccatg tgatgcaata tcgggggagt ggttctatct aatccttaat actctcgata
     5101 tgtcgaagta tcaatcagga acctcaatac ccgcgataaa tcaaagtgtc ataggtagta
     5161 taccaattgc gcttccttct ttaaaaatgc aagagaagat tgtttcttat gtcataaccc
     5221 taatgtccct ttgcgaccaa ctggaacagc aatccctgac cagtctggac gcacatcagc
     5281 aactggttga aaccctgttg ggaacactta cagacagcca aaacgccgag gaactggctg
     5341 aaaactgggc gcgtattagc gagcatttcg acaccctatt taccaccgaa gccagcgtgg
     5401 atgcgttaaa acagaccatt ctgcaactgg ccgtaatggg caaacttgtg ccgcaggatc
     5461 cgaatgacga accagcctct gaactgctca aacgaattgc gcaggaaaaa gctcaactgg
     5521 tgaaagaagg aaaaataaaa aaacaaaaac cgttgccgcc aattagcgat gaggaaaaac
     5581 cgtttgaact tccggaaggg tgggagtggt gtaaatttgg tttaacatca gagtttataa
     5641 atggcgatcg tggaagtaat taccccaata aaaatgagta tgtttctcaa ggtattcctt
     5701 ggatcaatac tggacatata gaaaaaaatg gtacacttac ggttacagaa atgaatttta
     5761 taaccgaagg aaaatttaat gaattaagaa gtgggaaaat tcaaaaaggt gatttggttt
     5821 attgcctacg aggagcaact tttggtaaaa ctgcctttgt aatcccctat gaaacaggag
     5881 ctattgcctc atcactaatg atcattagac catttatcac agaaatggga gggtatattt
     5941 ataattattt aaccagtcca tttgggcgct cacagattta ccgctttgat aatggctcag
     6001 cacaacccaa cctatcagca aatagtgtaa tgttgtattc attcccatgc ccaccattaa
     6061 ctgaacaata tagaatattt tcacaagtag gtttacttca tgaactatgc gataagctta
     6121 aaactcgtat aaaaacagcc caacaaaccc aactgcacct tgcagacgca ctcactgacg
     6181 cggcgataaa ctaattttct cgccctcaaa aaaccaataa agcggctaaa tggccgcttt
     6241 attcacatca ccaaaaatta tatttcacgc taattttcat cttattttca ttaattttta
     6301 gctggacttt ccctgtattt actgatgata tatccaggta tttagcgcgg tgcggatgtg
     6361 cgccaacaca cccgcaccgc taatcacaat cactattatc ggagaatagc agttatgact
     6421 gacacgcatt ctattgcaca accgttcgaa gcagaagtct ccccggcaaa taaccgtcaa
     6481 ttaaccgtga gttatgcgag tcgctacccg gattacagcc gtattcccgc catcaccctg
     6541 aaaggtcagt ggctggaagc cgccggtttt gccaccggca cggcggtaga tgtcaaagta
     6601 atggaaggct gtattgtcct cactgcccaa ccacccaccg ccgaagagag cgaactgatg
     6661 cagtcgctgc gccaggtgtg caagctgtcg gcgcgtaaac aaaagcaggt gcaggatttt
     6721 attggggtaa ttaccggtaa acagaaagtc gcctgataaa ttgacccggt agcgccagtg
     6781 ctaccgggta ttattcgccg cgttatacca acgcgctctc catctgtttt tcaaatgcct
     6841 gacgaattaa tcccataatg taaggaagtt gtgcgaggtc gctgaaacca atttctacat
     6901 cgccattacc ccagcgccca acatttgtca catctttggc aatacctttc ggatcgacca
     6961 gttcatgaaa ctgcatatta agcgacaacc gcagtcggct tttttgcggt acaacatcaa
     7021 caaaattcgt ttcagcttta aacgcaatat agagcttcaa tacttcctgc gtgatccctg
     7081 cgtccaggcg cataacttca tcgcgaagat gatcgaataa cacccggcta tgcgaaccat
     7141 cagcaagaaa aggataatca ctcaggctgt aactggtttt gttttcaggc tgcccccgat
     7201 aaacagctaa aacctcttca gaaagagaag gcgcttgcca gacctgaacc gcgagatcgg
     7261 ccagacgatc ggcacgggcg cgaatggcgg cttcatccca tttttcgcac tgcccaagac
     7321 cgatattcaa atagagcggg ctatgtttaa agccgtcttc aatatcgcgt ttttccgcga
     7381 aaggtctgtc actgtagcgg gagttatagc gcgttaaagt gagattcccc aacgtatgta
     7441 acagttcttt atgcacacgc tgccagtcgc ttcccagctc ttcacgccat ttagccgaca
     7501 gattttcgtt ctgcggcata atgtgctcaa tcgtaaactc ttccacgcgc tctctgcgtt
     7561 tatcgttttc cagtcttcgt aaccagtagc tgcggctgcg gaagttatag agatcgcgaa
     7621 cttttaattc ccgtttaaat tcatcatcgt tggggaaacg acgatatgaa ggcaggttca
     7681 taaaatgtac ctgaatactt tccagatatt tttctttatt aatgacctta taaaaagtgg
     7741 caaacgtctt gttcaaagaa ttcgtcggaa ttgcacatac tgcacggcgg aaaacataag
     7801 attcaattaa acgaattatg ctcaggaaat cttcgtgaga caaatcgtca tttttatagt
     7861 catgataaag cgccagtaag aaaggatacg ccacatcaac ctttaactcg cgcaaatcct
     7921 gaaaagccgt agcaagcgat ttgtcacttt cttttcccaa tgccattgca cagtaatact
     7981 ccgcgtagat atgaatatct tcaaccagcc gatctacgcc tttttcagca acactctggc
     8041 tgcgggcatg gagtttaaat gcctcataga cttcatctgt ccgagggatc tcccccgttt
     8101 ttaccgtcag ataatgacgc ataaaactgt caaaatattc gctgtaacct tgctgaccaa
     8161 aggcgacttc cattggacgc cagtgatctt catacaaccg ggtttgatgc tctggttcga
     8221 ggcccatcag aataaaattg cgcaccagat cggcctgact taacgcctta ccggtggagt
     8281 tcatactttc aaaaatcagt tgcggattat cctgaccacg attaagcgcc acatcgacaa
     8341 ttaataactt tgctaaccca cgacataagg ggatcaagtc atcaccgagt ttggcaattt
     8401 gttcatcaaa gaaagtaaag ttttccatta ttcgatggga atagttttct ggcattggtc
     8461 tttgttttat taacgccagt aaactgtctt tatcagtctc ggtcagtagt aatttaaaac
     8521 ctttctcgcc ggactcataa ggattgagca aataataatt acgcaatttc attgctgaaa
     8581 agccatcaaa aacttcgtct tcgccaagat ggcgcgataa agcctcaatc agcaacattg
     8641 cggtcgtcag ccgttgttga ccatcaatga ccagtaacgg agaaatacca gaaacctgat
     8701 acaacccctg ctcaatataa acaaccgaac cgataaaatg cgctgatata tcgtcacgct
     8761 ttccggcacg aatgatgtcg tcccaaagtt gccgacattg ttgttctgtc catgaataag
     8821 tccgttgata aatgggaata acaaactgct gcgaacgttt taaaaaatcg agtaggcggg
     8881 cttctgtagc tttcattctg acgtctttcc gtgaagcgat gatttcgcaa taataaccac
     8941 ttccttttgg aggttataga gaaatcctgt ttatccgcga tgaattgccc cctcactcca
     9001 catcctccct gcgtaaccca atatctctca accgctcatc actcatctgc tgtaacactc
     9061 gtcgggtctg catttgccgc cgccaacgcc tcaccgcctg ccagagttgc acaagtctga
     9121 taaacggcgt tttggctttg ttttcgtgaa attccatctg ctgcctcctc atctttcaga
     9181 ggcttcatca tccaccttct ggcagttcac aatacagatg caaatattag ttttatttaa
     9241 catacagaca ggttaaaaca gcatctgcat ccggtttttt agcctcatct gtaccggttt
     9301 ttctttctgt atggtccaaa taaggaatac agcatgacgc gttatcaaca tctggcgact
     9361 ctacttgccg agcggattga gcaagggctg tatcgtcatg gggagaaatt gccgtcggtg
     9421 cgtagcttaa gtcgggagca cggcgtcagc atcagcaccg tgcagcaggc gtatcagacg
     9481 ctggagacga tgaagctcat aaccccacag ccgcgttcgg gttattttgt cgcacaacgt
     9541 aaagcccagc cgccagtgcc gccgatgacg cgtccggtgc agcgcccggt ggaaattacc
     9601 cagtgggatc aggtgctgga tatgctggtg gcgcatagcg acagttccat tgttccgtta
     9661 agcaaaagca cgccggatgt cgaaacgccc agcctgaaac cgctctggcg ggagctaagc
     9721 cgggtggtgc agcataatct gcaaaccgtg ctcggttatg acttgttagc cggtcagcgg
     9781 gtattgagag agcagattgc ccgcctgatg ctcgacagcg gctcggtggt caccgccgat
     9841 gacatcatta tcaccagcgg ctgccataac tcgatgtcgc tggcgttaat ggcggtgtgt
     9901 aaaccgggcg atattgtcgc ggtcgaatct ccctgttatt acggttcgat gcagatgctg
     9961 cgcggcatgg gcgtgaaagt gattgaaatc ccaaccgatc cagaaacagg catcagcgtt
    10021 gaagcgctgg aactggcgct ggaacagtgg ccgattaaag gcatcattct ggtgccaaac
    10081 tgtaataatc cgctgggatt tattatgccg gacgcacgca aacgggccgt tctctctctc
    10141 gctcagcgtc atgatattgt gatttttgaa gatgatgtct acggcgaact ggcaacggag
    10201 tatccgcgcc cgcggaccat ccattcatgg gatatcgacg ggcgagtgct gttgtgcagc
    10261 tcgttcagta aaagtattgc tccaggcctg cgcgtgggtt ggggcgcacc ggggcgttat
    10321 cacgataaac tgatgcatat gaaatacgcc atcagcagct ttaatgtgcc gtccacgcaa
    10381 atggcggcgg caacgtttgt gctggaaggt cactatcatc gccatatccg gcggatgcgg
    10441 cagaactatc agcgcaattt ggcgctttat acctgctgga tacgggaata ttttccctgc
    10501 gaaatctgta ttacgcgccc gaaaggcgga tttttactgt ggatagaatt gcctgaacag
    10561 gtcgatatgg tctgcgttgc gcggcagctg tgccgcatga atatccaggt ggcagcaggc
    10621 tcgattttct cggcttccgg caaataccgt aattgtctac gcatcaactg cgctttgccg
    10681 ctcagcgaaa cctatcgcga agcactaaag caaattggcg aggccgtgta tcgggcaatg
    10741 gaataaattc gaattagtgg cgaagcacct gatttcctct cacgaatcgc ctgtaactcg
    10801 ttgtatcttt gcagggtcat cgccacgttc cagcatggct taggcaaagt gtgcttcgtt
    10861 cataccggat gcggcgtgaa cgccttatcc ggcctacaaa ccgcgcaaat tcaatagatt
    10921 gcagaggaaa tgtaggtctg ataagcgtag cgcatcaggc agctttgcgt ttgtcatccg
    10981 tctctgatta gtattcgatc catggatcgc gcttgatttc aggcaagggc tgctctaatg
    11041 cctgcatttg ctctgtcgaa agtccggtaa tcttttgtat ccattcagga tctgccccat
    11101 tctgcaacaa caatctaagg aggcgttgtt ccccttcttc tttccccttt tccattccca
    11161 acagccatcc atcattataa attcgctctg caatcgtcat tattcgctcc ctgtgttgtg
    11221 gcattcgacg ggtaagttcg ctgataaact tcttaaaacg cgcttcatcg ccagtcagta
    11281 aaatgtaatt taacagcgca gttatctggc tgtcattagc acactctgta accagtaata
    11341 ctaccagttg gtcgataagc cccatcagat cgcgctggcg aatatgcttt tggatcaact
    11401 ccaacagggc gactctgcga tgctgcacaa tctcgtcgtc tggcacgaca gtgatatcca
    11461 ccagcgggaa cgcggcggta taaagtttcc gtgcggtagt tgggtcggca aattcgtcca
    11521 gccagcacag ggaccaggga taaggactac ggctaccgtg ataaaatagc atcggtataa
    11581 ccagcggtag cgcccgacgt ttatcatgct cgatatggcg ctgcatcacc gccatggaat
    11641 agcgcatcag gcgaaaggcc atatgaatat cctcacggct ctgatgttca atcaccacat
    11701 aaatatagcc atcgccttcg cgggtattta ccgaccacag aatatcggag tgtagcgccc
    11761 gcaatttttc atcgacaaag ctggcggatt ccagttttaa gctgtcgaga tcgcacagtt
    11821 cacgtaaatc tttgggtaaa tagatctcca taaaatcccg cgcggtgtca gggtgggtga
    11881 gaaaggattt aaataatgcg tcatgcggcg tgctggttgt gaagtttgtc atggcgatcc
    11941 gtcacctgtt gataagatgg cccgacttta gcgactaaca aaaccattca cacctggcaa
    12001 tttcccgttc cccgaggcgc tttccaggct atttttgttc ctctgcaagc gatacatttt
    12061 tccggaaaca ggctcgcgtt ttacatttcc tgtacccggt agatattccc ctttccaccg
    12121 cgctcttttg attaatcgca aattttaatt acacttaagg tgtatatttt ctatgcaaac
    12181 catcaattca agaggtgtaa tgtgctgatg actatttgta atcgttatac attctgaccc
    12241 gaactcagaa ggtatttctc tgtctgtata agcacaggca gtgtggttga ttacatgaaa
    12301 ttcagtacat ttgtccttct cgcctacctt tcgtcattac cgacggtatt gaatttcgtt
    12361 ttccccgttg gggttctccg gacaaggagt tgtttgttat gccacgtttt tttgcccgcc
    12421 atgccgccac gctgtttttc ccgatggcgt tgattttgta tgattttgcc gcgtacctgt
    12481 cgacggacct gattcagccg gggatcatta atgtggtccg tgattttaat gccgatgtca
    12541 gtctggcccc ggcagccgtc agtctctatc tcgcgggcgg tatggcgtta cagtggctgc
    12601 tggggccgct ttccgacagg attggccgca ggccggtgct gattaccggg gcgctaatat
    12661 ttacccttgc ctgcgctgcg acaatgttca caacgtctat gacacagttt cttatcgcgc
    12721 gtgcaattca gggcaccagt atctgtttta ttgctaccgt tggttatgtc acggtgcagg
    12781 aggcgttcgg acagacaaaa gggatcaagt tgatggcgat tatcacctcc atcgtactga
    12841 ttgcgccgat tattggcccg ctttccggcg cagctctgat gcactttgtg cactggaaag
    12901 tcctttttgc catcattgcg gttatgggtt ttatctcatt tgttggctta ctgctggcga
    12961 tgccagagac ggtgaagcgc ggcgcggttc catttagcgc caaaagcgtc ttgcgcgatt
    13021 ttcgtaatgt cttttgcaat cggctgttcc tctttggcgc agcaaccatc tctttaagct
    13081 atatcccaat gatgagttgg gtggctgtct cgccggtgat cctgattgac gcaggcggct
    13141 taacaacttc gcagttcgcc tggacacagg ttccggtgtt cggcgcggtg attgtcgcga
    13201 atgccatcgt ggcgcgtttc gttaaagatc cgaccgaacc gcgctttatc tggcgtgccg
    13261 tacccattca actggtcggc ctcgcactgt tgattatcgg caatctgctg tcaccgcacg
    13321 tctggctgtg gtcggtgctg ggcaccagtc tgtatgcttt cgggattggt ttaatcttcc
    13381 cgaccttatt ccgctttaca cttttttcca ataacttacc gaaagggacc gtctccgcat
    13441 cgctgaatat ggtgatcctg atggtgatgt ctgtctcggt cgaaatcggc cgctggctgt
    13501 ggtttaacgg cggtcgcttg ccgtttcatc tgttagccgt cgtagcggga gttatcgtcg
    13561 ttttcaccct ggcggggttg ctcaatcgcg tgcaccaaca tcaggctgcc gagctggcgg
    13621 aggagcggtg atttttgcgc gatcctgccg tcaggctcta ttcttaacgt tatgaataaa
    13681 ctcattgaac tcagacgcgc caaaatgttg gcgctctctt tactgcttat cgccgctgct
    13741 acctttgtcg ttacgctgtt tttgccaccc aatttttggg tgagcggcgt gaaggcaatt
    13801 gctgaagcgg cgatggtcgg cgcgctggcg gactggtttg cggtggtggc gctgtttcgc
    13861 cgcgtgccaa ttccgatcat ttctcgtcat acggcgatta tcccgcgtaa taaagaccgg
    13921 attggcgaaa atctcggtca gttcgtgcag gaaaaatttc tcgataccca gtcgctggtg
    13981 gcattgattc gacgtcacga accggcgttg ctaattggta actggtttag ccagccagaa
    14041 aacgcccgcc gcgttggtca gcatctgttg cagatcatga gtggttttct tgaactgacc
    14101 gatgatgcac gtattcagcg cctgcttaag cgcgcggtcc atcgggcgat tgataaggtc
    14161 gatctttccg gaaccagtgc gttgatgctg gagagtatga ccaaaaacga tcgtcatcag
    14221 gtgctgctcg atacgctgat cgcacagttg attgctcttc tccagcgcga taaatcgcgc
    14281 aagtttatcg cccaacaaat tgttcgctgg ctggagagtg agcatccact gaaagccaaa
    14341 attctcccca ccgaatggct gggcgaacat agcgcggagt tggtttctga cgcggtgaat
    14401 tctttgcttg atgatatcag ccgcgatcgt gcgcatcaga tccgccatgc atttgatcgc
    14461 gccacttttg ccctgatcga caagttgaaa aacgatccgg aaatggcagc gcgagctgat
    14521 gccgtaaaaa gctatctgaa agaggatgaa gcctttaacc gctatctcag tgaattgtgg
    14581 ggggatttac gggaatggct gaaagcggat atcaacagtg aagattctcg tgtgaaagaa
    14641 cgtatcgcgc gggcgggtca atggtttggc gaaacgttaa ttgccgatga tgccttgcgg
    14701 gcgtcgttaa atggtcatct ggaacaagcc gcgcaccgcg tcgcacctga gttttccgca
    14761 ttcctgacgc gccacatcag cgatacggta aaaagctggg atgcccggga tatgtcgcgg
    14821 caaatagagt taaatatcgg caaagatctg cagtttatcc gtgtcaacgg tacgctggtt
    14881 ggcggttgta ttgggctaat tttatatttg ctgtcacagc tcccggcctt gttccccctc
    14941 ggcaattttt agaaatccat gaaaaactaa gtggctaacc gcgagagaga tcaaataaac
    15001 cacactaaaa gaatggtaat aatgtggccc cttttcatta tctcccgtgg tacggggaag
    15061 gaaaatcatg tcacttgtca ccgatctacc cgccattttc gatcagttct ctgaagctcg
    15121 ccagaaaggc tttctcaccg tcatggatct caaggagcgc ggcattccgc tggttggcac
    15181 ttactgcact tttatgccgc aagagatccc gatggcagcc ggtgcggttg tggtttcgct
    15241 ctgttccacc tctgatgaaa ccattgaaga agcggagaaa gatctgccgc gcaacctctg
    15301 cccgctgatt aaaagcagct acggcttcgg taaaaccgat aaatgcccct acttctactt
    15361 ttctgatctg gtggtcggtg aaaccacctg cgacggcaaa aagaaaatgt atgaatacat
    15421 ggcggagttt aagcctgttc atgtgatgca gctgccaaat agcgttaaag acgatgcctc
    15481 gcgtgcgtta tggaaagccg agatgctgcg cttacaaaaa gcggtggaag aacgttttgg
    15541 gcacgaaatt agcgaagatg ctctgcgcga tgccattgct ctgaaaaacc gcgaacgtcg
    15601 cgcactggct aatttttatc atcttgggca gttaaatcct ccggcgctta gcggcagcga
    15661 cattctgaaa gtggtttacg gcgcaacctt ccggttcgat aaagaggcgt tgatcaatga
    15721 actggacgcg atgaccgccc gcgttcgtca gcagtgggaa gaaggccagc gactggaccc
    15781 gcgtccacgc attttaatca ccggctgccc gattggcggc gcagcagaaa aagtggtgcg
    15841 cgcgattgaa gagaatggcg gctgggttgt cggttatgaa aactgcaccg gggcgaaagc
    15901 gaccgagcaa tgcgtggcag aaacgggcga tgtctacgac gcgctggcgg ataaatatct
    15961 ggcgattggc tgctcctgtg tttcgccgaa cgatcagcgc ctgcaaatgc tcagccagat
    16021 ggtggaagaa tatcaggtcg atggcgtagt tgatgtgatt ttgcaggcgt gccataccta
    16081 cgcggtggaa tcgctggcga ttaaacgtca tgtgcgtcag cagcacaaca ttccttatat
    16141 cgctattgaa acagactact ccacctcaga tgtcgggcag ctcagtaccc gtgtcgcggc
    16201 ctttattgag atgctgtaag gagtggcagt gacatattcg attggcattg attctggttc
    16261 taccgccacc aaagggatct tactggcaga cggcgtgatt acgcgccgtt tcctcgttcc
    16321 aacacccttt cgtccggcaa cagcaattac tgaagcctgg gaaactctgc gcgaagggtt
    16381 agagacaacg ccgtttctga cgctcaccgg ctatggacgg caactggtgg attttgccga
    16441 taaacaagtg acggaaatct cctgtcatgg gctgggcgca cggtttcttg cgccagcaac
    16501 gcgcgcggta atcgacatcg gtggtcagga cagcaaagtg attcagcttg atgatgacgg
    16561 taacctgtgc gatttcctga tgaatgacaa atgcgcggcg ggcaccgggc gtttcctgga
    16621 ggtgatctcg cgcacgcttg gcaccagcgt cgagcaactc gacagcatta ccgaaaatgt
    16681 cacgccgcac gccatcacga gtatgtgcac agtgtttgct gaatcagaag cgatcagcct
    16741 gcgctcagcg ggcgtcgcgc cagaagcgat tctcgccgga gtgattaacg cgatggcgcg
    16801 gaggagtgcc aatttcattg ctcgtctctc ctgtgaagcg ccgattctgt ttactggtgg
    16861 cgttagtcat tgccagacgt ttgcccggat gctggaatct catctgggaa tgccggtaaa
    16921 tacccatcct gatgcgcaat ttgctggcgc aattggcgcg gcggtaattg gtcaacgagt
    16981 gaggacacgc cgatgaaaga gtttttattt ttatttcact ccacagtcgg cgtcatacaa
    17041 acccgcaaag cgttgcaggc agcgggcatg acttttcgcg tcagcgatat tccccgtgat
    17101 ttacgcggcg gctgcgggtt atgcatttgg ctgacctgtc cccccggcga ggaaatgcaa
    17161 tgggtgatcc ctgggctgac tgagtcaatt tattgtcagc aggatggtgt ctggcgctgc
    17221 attgcacatt accgggtttc tccgcgttaa ggattcatta aggtcgctac gtatacttct
    17281 cgatatctta ttgatttgcc ttaaagggat taccgtgaca aaaagtattt cgttaagtaa
    17341 acgcatctcc gttattgtga ttctttttgc cattgtggca gtgtgtactt tcttcgtgca
    17401 atcttgcgcc cgtaaaagca atcatgcagc ataatttcag aattaccacg ccaccattga
    17461 cggcaaagaa attgcaggca taacgaataa tatctcttcg ctgacctggt cggcacaaag
    17521 caatactctt tttagcacta tcaacaaacc tgccgccatt gttgaaatga ccaccaatgg
    17581 tgattttatc cgcacaattc ctctggattt tgttaaggat ctcgagacga tcgaatacat
    17641 tggcgataat cagtttgtca ttagcgatga gcgcgattac gccatttatg tgatttcact
    17701 gaccccaaac tcggaagtaa aaatcctcaa aaaaataaaa atcccgctgc aagactcccc
    17761 aaccaattgc ggttttgaag ggttggctta ttctcgccag gatcacactt tctggttttt
    17821 taaagagaaa aatccgattg aggtctacaa agttaacggg ctgttaagta gcaatgaatt
    17881 acacatcagc aaagacgagg cactacaacg gcaatttact ttagatgatg tgtccggcgc
    17941 agaatttaac cagcaaaaaa atacgcttct ggtgctgtct catgaatctc gcgcactcca
    18001 ggaagtaacc ctggttggcg aggttatcgg tgaaatatca ctaaccaaag gtagtcgagg
    18061 tttgtcgcat aacatcaaac aggccgaagg gattgcgatg gatgcctctg gtaatattta
    18121 tatcgttagc gaaccaaatc gtttttatcg ctttacccct aaatcatcgc attaacaggc
    18181 tggcatattt cacctttttg ttattaactt gttaacaaca aaaatgacat acaaggttaa
    18241 acatgccttc gtcctcgcat cccgtagaac gcttttcttt cagcaccgcg cttttcggga
    18301 tgctggttct gaccttaggt atgggattag gtcgcttcct ttatacgcct atgttgcccg
    18361 tcatgatggc ggaaggggcg ttttcattta gccagctctc gtggattgcc agcggcaact
    18421 atgcggggta tctggctggc agtctgctat tttcgtttgg cgcatttcac cagccgtcgc
    18481 gcctgcgccc atttctgttg gtttctgccc tggcgagcgg gttgttgatc ctcgcaatgg
    18541 catggttgcc gccatttatt ctggtgttat tgattcgctt cctggcgggt gtcgccagcg
    18601 ccggtatgct gatttttggt tcgacgctga ttatgcagca cacgcgccat ccttttgtgc
    18661 tggcagcttt gttttctggc gttggcattg gcattgcact gggcaacgaa tatgttctgg
    18721 caggcctgca ttttgcctta tcctcgcaaa cgttatggca aggcgccggg gcgctttctg
    18781 gcatgatgct gattgcactt acgcttttaa tgccctcgaa aaaacacgcc atcgcaccaa
    18841 tgccattggc aaaaacggag caacagataa tgagctggtg gttactggct attctgtacg
    18901 gcctggcggg atttggttat atcatcgtcg cgacttacct gccgctcatg gcgaaagatg
    18961 caggctcacc attgttaacc gcccatctct ggacgttagt cggcttatca attgtgccag
    19021 gctgctttgg ctggctatgg gcagcaaaac gttggggggc tttgccttgc ctgacggcga
    19081 atttgctggt gcaggctatc agtgtgctgc ttactctcgc cagcgactct cctctcttgc
    19141 ttatcattag tagtatcggt tttggcggta cctttatggg cacgacatct ctggtgatga
    19201 ctatcgcccg ccaactgagc gttccgggaa atctcaatct tttaggcttt gtgacgctca
    19261 tttacggcat tggacaaatc ctcggcccgg cgctgaccag tatgctcggc aatggaacat
    19321 cggcgcttgc cggcgccact ctctgcggcg cggcggcgtt gtttattgct gcgcttatca
    19381 gcacagtaca attatttaag ctgcaagtgg tcacttcatg aggagtgttt cccttaccac
    19441 tcaagaaatt cgaccgcaac cgttgatgtc agccagaccc cgttttccgt ttgccagaaa
    19501 gggatgcctc gtttcgccat ttcctgtgct ttgacggtta agataaccgg cgatccgtgt
    19561 cttgctccca ctttgcgtgc cgttgcttca tcagcagaga gatgtacgta gtgacgctca
    19621 cctgcaatca gcccctgttt ttttatctcg tcaaggaaac ggcttgccgt accgtggtaa
    19681 agaaactgtg gcggtgtttt ttcggcaaag gaaatagcga cctgcgaagt caaatgcccc
    19741 tgaaccgcgc ggatgcacct accatcactg gaataactaa aacgcttttt atcactggtg
    19801 gcgaccacgg tatccagcaa tgtacgggtt aatcgcttgc ccgctttttg ggcacaaagt
    19861 atgagcttat cgatatccgc ccatccttca cggtcaagta caataccaat cgcctccggt
    19921 ttgtgtcgga ggacaaaact taaaaacttg ctcgtatccg ccagctcttt ttcgttgtac
    19981 tttgccatct cgttttcgtc cctggatttt ctgcttttat ttactgatgt cattgtcact
    20041 gggtttatga attttctgca actcaatcct gcgcagagcc ttttccacac agcgtaattt
    20101 ttccgctatg caaaaaacgc atagccagaa acatacttgc attttcccca tacccacatg
    20161 gccgataagg tttttcagct ccaggaattt tcctggctca agcaacacac aaacacaaaa
    20221 aaacaatggg gattgttatg acacaacaag gggatgctgt agccggggat ctggcgacag
    20281 aaaaagtggg cattaaaggg tatctggctt tttttctcac cataatcttt ttttccggtg
    20341 ttttttccgg gacagacagc tggtggcgcg tttttgattt ttccgttctg aacggatcgt
    20401 ttgggcaact gcctggtgcg aacggagcca ccacctcatt tcgcggggcg ggtggcgcgg
    20461 gggctaaaga tggctttctc tttgcgctgg agctggcacc gtcagtcatt ctctctctgg
    20521 ggattatttc aattaccgat ggtcttggtg gattacgcgc cgcgcagcag ttaatgacac
    20581 ctgtgctaaa accgttgttg ggcattccag ggatctgctc gctggcgctg atcgctaacc
    20641 tgcaaaacac tgatgccgcc gccggtatga ccaaagagct ggcgcaggaa ggggaaatta
    20701 ccgaacgcga taaagtgatt ttcgccgcat atcaaaccag cggtagcgcc atcatcacca
    20761 actacttctc ttccggcgtc gcggtgttcg cctttctggg cacgtccgta atcgtacctt
    20821 tagccgtcat cctggtgttt aaatttgtcg gagccaacat tttgcgcgtc tggcttaact
    20881 tcgaagaacg tcgcaaccca acgcaaggag cgcaagcatg acaactcagg tccgcaaaaa
    20941 cgtcatggat atgtttatcg atggcgcgcg ccgtggcttt actatcgcca cgaccaacct
    21001 gctgccaaac gtggtgatgg cgtttgtcat cattcaggcg ctgaaaatta ccggcctgct
    21061 cgactgggtg gggcatatct gtgaacccgt aatggcgctg tggggattac ccggtgaagc
    21121 ggcaaccgtc cttttagccg cattaatgag tatgggtggc gcagtcggtg ttgccgccag
    21181 tctggcaacc gctggcgcat taaccggtca cgatgtcacc gttttactgc ccgcgatgta
    21241 tttaatgggc aacccggtgc aaaatgtcgg tcgctgcctg gggacagccg aagtgaatgc
    21301 caaatattac ccgcatatca ttacggtctg cgtgattaac gcattgctgt cgatctgggt
    21361 tatgcagctt attgtttaac aaggagttac catgattgat tataccgcag ccggttttac
    21421 cctgctgcag ggagcgcatt tgtatgcgcc ggaagatcgg ggaatttgcg atgtcctcgt
    21481 cgctaacggc aaaattatcg ccgttgccag caatatccct tctgacattg taccggactg
    21541 cacggttgtc gatctcagcg ggcagatcct ctttcctggt tttattgatc aacacgtcca
    21601 tttgattggt ggtggcggtg aagcaggtcc cacgacgcgc acgccagaag tggcgctaag
    21661 tcgcctgacg gaagcgggcg tcacgtcagt ggttggtctg ctgggcaccg actctatctc
    21721 tcgccacccg gaatccctgc tcgccaagac ccgtgcgctc aatgaagaag gcatcagcgc
    21781 ctggatgctg accggcgctt atcatgtccc ttcccgcacc attacaggtt ccgtggaaaa
    21841 agacgtggcg attatcgatc gtgtgattgg cgtgaaatgc gccatctctg atcaccgttc
    21901 tgccgcaccg gacgtttatc acctggctaa tatggcggcg gaatcccgcg ttggcggttt
    21961 gctcggcggt aaacctggcg tcaccgtgtt ccacatgggc gacagcaaaa aggcgttaca
    22021 gcctgtctat gacctgctgg aaaactgcga tgtgccgatc agcaagttgc tgccgaccca
    22081 cgttaaccgc aacgttccgt tgtttgagca ggcgctggag ttcgcgcgca aaggcggcac
    22141 tatcgatatc accagcagca ttgacgaacc ggtcgcccct gccgaaggta ttgcccgcgc
    22201 cgttcaggcg ggtattccgc tggcacgcgt caccctcagc tccgacggca acggtagcca
    22261 gccgttcttc gatgacgaag ggaatttaac ccatatcggt gttgccggtt ttgaaacgtt
    22321 gctggaaacc gtgcaggtgc tggtcaaaga ctatgatttc agtatcagcg atgccctgcg
    22381 cccgctcacc agtagcgtag ccggtttcct taacctgacc gggaaaggcg aaattctgcc
    22441 aggcaatgat gctgacttgc tggtcatgac gccagaactg cgcattgagc aggtatacgc
    22501 tcgcggcaaa ctgatggtca aagacggcaa agcctgcgtg aaaggaacgt ttgaaacggc
    22561 ttaaacggtt gtcagccgcc cgaatggacg tagaatgccc gcaagggcgg ctgacagagt
    22621 aaaacgtaat ggatgactgt ggtgcgattt tgcataatat tgaaaccaaa tggctttatg
    22681 attttctgac cctggaaaaa tgccgcaatt tttcccaggc ggcagtcagt cgcaacgtct
    22741 cgcaaccggc attcagccgc cgcatccgtg cgctggaaca ggcgattggt gttgaattgt
    22801 ttaaccgcca ggtaacgccg ctgcaactct cagaacaagg gaaaatcttc cattcgcaga
    22861 tccgccatct gttgcaacag ttagagagca atctggcaga gctgcgtggc ggcagcgatt
    22921 acgcgcaacg taaaatcaag atagccgctg cacactctct ttccctcggg ctgttaccgt
    22981 ccattatcag ccagatgccg ccgctcttta cctgggcaat tgaagctatt gatgtcgatg
    23041 aagcggtcga taaactgcgt gaagggcaaa gtgactgtat tttttccttt cacgacgaag
    23101 atttgctgga agcgccgttt gaccacattc gcttatttga atctcaattg ttccctgtct
    23161 gcgccagtga cgaacacgga gaagcacttt ttaacctcgc gcagccacac tttccgttac
    23221 tgaattacag ccgcaactcc tacatggggc gattgattaa tcgcaccctg acgcgccaca
    23281 gtgagttaag tttcagcacc ttttttgtct cttcgatgag cgagctttta aagcaggttg
    23341 ccctcgacgg ctgtgggatt gcctggctgc cggaatacgc tatccaacaa gaaattcgca
    23401 gcgggcagct cgttgtgctg aatcgggacg aactggtgat cccgatccag gatctacgca
    23461 taccggatga atactcgcat gaatcccgtt gccgaacgct tctggcgtga actgcgcgag
    23521 ctggagattg tgcttagctg acattctcca gcatcgcact acaccaaccg ccattatccc
    23581 agaccagatc aaacgttagc ggttcaccgt cgtggtacag cattatctca aggctgatcg
    23641 ccagagtgtg gcagtacgct tctttaaaat taacctgcaa cgccgcaatc cgtggctcat
    23701 gaaataacag tgcagaacgc agctgacggc aataccagct ctggagatgt ttgtcgctct
    23761 ggttatgcac ttgccactcg ggcatgcctg aaacaagtaa tgacggtaat gacgaatgag
    23821 gagaattaaa aagagtgagg agatcgctgc ataccgattt acgcagcaat gacgttttat
    23881 tgcctgaaat ttcaggtaaa acagcctgac gtgattggcg catcattttg cgcactcctg
    23941 acgtttagca acatatgcgg cttgccctga acaccgggca ccactgtatt gatgctcatt
    24001 ttccatagcc attccctttt gttcattaca tccatgtcag tctgagacag gcctgaatcc
    24061 tgtcagcaaa gtataaaacc ttacttgcct atgaatatct actgactttt caggaaaaaa
    24121 gtcatgattt gataaaaatc atcaaactca aaaaaaaacc gctagtatat tctgaatatt
    24181 ccgaatagtt acgtagatga aaatacttgt caccaccgct acttaattcc ggtgtgaaat
    24241 tatttatttc cgcataatca gccatgtatt atttgaaaca attagtttgt aaataatata
    24301 aattgataac atatcaaata catcaaaaat accattcaca ggcattatta atatagacat
    24361 caatatagca acatcaaatt ccaattagta ataagatatg cttttaaatt gcatgaaaat
    24421 aatttcaaat gagcgttttt tgatacttta gagtgtttcc taccctttaa tatcaccgtg
    24481 tagagttgat atcactacaa cagaaacatg catgtcattt gtttacttct cattttacta
    24541 cagaaaaatt tatctatttt tctaaacatc agtgaaatac tcataaaagg taactattat
    24601 gtctatgcca ttaagcaata cccttcagag ccaaattatt acagataatc acttccttca
    24661 tcatccaaaa gtcgacagtg agttaacacg taaatatgaa cgtgcacgcc tggatacgga
    24721 aaatatctat cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt
    24781 tgtcgaaatt agaaaacttg atattagtaa agagtcctgg ccatttaatt atgtgacaga
    24841 agcatgcaga gaatccgatg gtattacaac aacaggacgt atgctctatc gaaacttgaa
    24901 aatcacctca gctttagacg aaatatatgg tggcatatgt aaaaaggcgc atgctgctac
    24961 agaattagca gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga
    25021 gaactataca gttcatgaaa taacattagg ccctggttgc aatgttccag gttacgccgg
    25081 aaccacgata ggatatattt caacccttcc agtttctcag gcaaaaagat ggaccaatga
    25141 gcagccaaga attgatatct atattgatca gataataact gtctcagggg tagcaaattc
    25201 ttccggattc gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat
    25261 cattggtata gaagcctatc ctggtaccgc ggagatccat gcaaaaatgg gatataaagt
    25321 tatccccggt gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc
    25381 agaactgttt gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag
    25441 aatgagtact aatattcagg ttatacagat atttaaacaa taccctgcaa tttacaaagc
    25501 aatactcatt gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat
    25561 tataataagt cagacgtcga ctttatccag cggccatgaa tcaaacagat agccgtcaaa
    25621 ataaatatcg tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg
    25681 ccacattgcc agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt
    25741 gtgatcgccc aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat
    25801 ccacattgga ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct
    25861 gttatgcgtt gcttcggcaa tagcgagatg gaactgcatg tcaccgcttt cgctgctgcc
    25921 cggcgcactg gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat
    25981 atcttcgcgg gtagcctgca aagcggcaaa ctcggcgatg ttgctctcca ataactgccg
    26041 cgcctgtaac agctcaaaag gaccggcatc gttgcagacg ttggcatccg gactgtctgt
    26101 gttctggctg cctgagttat caagaacata gataccggca ccccggcgta cttccaccag
    26161 ccctttgatc tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat
    26221 ttctgcaatt tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat
    26281 cagatcgcgg atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tggcagattt
    26341 catggtgttg tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc
    26401 cttatccggc ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag
    26461 cactggaagt tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag
    26521 tttgattgac cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc
    26581 gcgcaccgcg ttcgcacagc tgctgatatg ccgccgtcac tgcgccaaca aagtcggcat
    26641 tctgcggcag atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg
    26701 cgccctgata ctgcgcgttg accttctgga actccgccag catcgggtcg accacgtcaa
    26761 tggcattacc ctgctcatcc acgccctggg tgtaacgcat ccagccagcc acgcccagcg
    26821 ccaggtgacg ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agcagacgct
    26881 gcggtaactt ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt
    26941 tgctgaaacg ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg
    27001 acagcgttgg cgcttgttcc tgcatcatca aggagaaggc cgctttgcga taatccgggt
    27061 tagtcatggt gtcggcaatg gtttcatagc cgccgaggta accgaggtat gccaggaaag
    27121 agtggctgcc gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga
    27181 actgtgcgcc cactttatcc cagtccgggc gaccgttaac gaagttatct tcaatcaccc
    27241 actgacggaa cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa
    27301 tttcctgcaa ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga
    27361 aggtgacgtt ttcttcaatc catgccgcca gctgcgggtc acgcgcctga gccagtccca
    27421 gtaccgcgac cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa
    27481 acgctttcaa ccctttttca cgacgcagcc gcagggcttc gacgatgtaa ccaatcgcgg
    27541 acttcggcgc agtcgggttt tccagatcgt gcttgatcag tgggttattg agatccagct
    27601 gaccgcttgc cgcatcagcg cagtagcctt tttccgtgac tgtcagagag acaatcgccg
    27661 tctgcggacg cgccatcgca ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg
    27721 cttctttcat cgaaccgata attttcagct cggtgctttc cgcgcctttt tccgccacgg
    27781 tgtacagcag ttgctgtttt ttcaggtttt cgatcagcac gcggtcgttg cctggcatca
    27841 ggttaacttc gcagataccc cagtcgctgt cggtgctttc cagcaggtga tgggtataca
    27901 gcgcctggtg cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac
    27961 gagaatgctc ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg
    28021 taaactccag aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg
    28081 cagccatgcc ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc
    28141 gcgaacttcg gccaggcctt tcagacgacc aattgcggag taacctgggt tggttttctt
    28201 cttcaggtcg tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt
    28261 gccttccgct ttacgacggt gttcttcttc aacaatcgct ttcaccactt cgtacatatc
    28321 aacgtcaccg ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt
    28381 ggagcgcaga tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc
    28441 gttgtcagca cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt
    28501 tacggtatca accatccact gcatatcttc aatggtggaa acaatgcgcg gcaggccaag
    28561 gatcgggcgc ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac
    28621 tggaataatc gctttcagga agacggcaaa gttttcgcgc agcttcgctt tatcgatatc
    28681 tttgtacagc tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg
    28741 cagaccggca atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa
    28801 gcgaacagca gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt
    28861 caggatatgc atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc
    28921 gtctggcagc acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta
    28981 gcacacggtg cgaatgccgc actgcgccag gttgcgcaga gtctgctgat agttagcgat
    29041 ccactgctca tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac
    29101 gacagaccac accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac
    29161 ggaccatact tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc
    29221 ctgacggaca tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc
    29281 catgattttt cctctttcac atagtgggtt ccacagcaaa gagacgcgga accctggagt
    29341 tgtttaggta tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gattagatgg
    29401 cgtataagtt ctaccttttg gtaagacatg tcaacccgaa ttctgaaatt ggttaaccac
    29461 atcacaagaa tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg
    29521 ttattgatca gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt
    29581 cgcccaaaat aacaacggtt caaccaggta aggtaaaacg ttatgcatgt gcttaacatt
    29641 ctctgggtgg tattcggcat tggcctgatg ctggtactga caaagaccgc aggttcagac
    29701 acattcgctg cattagcaat gcctcttaga taattttgac ctgtcagtaa aagacacgtt
    29761 gaaaacctgg ggtctgctgg agctggtcaa ctccgtggtt gggctgatta ttagcaaggt
    29821 agcgtaaaag aacaccagag cctgccaatg gcaggctctg actgatgaca aagtctaaaa
    29881 atgtgcccgg acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc
    29941 agcaaagtgc gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa
    30001 gggctaacgt gcaggttttt agcttcaggt aatattgcgt aactgcatta gcaatgccct
    30061 gtgatttctt tattgataaa caaaagtcac gccaataatc gattgcacat tccctgcagt
    30121 cacctgccct ccggtacgtg cgtaatttgc cgttaatccc agacttaccg ccgaagtccc
    30181 tactgctcct aacgataccg tgttattcgc tggaataatc gtaccgttgc gcgtcaactg
    30241 tacgccgacg ccctgcgctg gtgaaaacga cgcggtattg gtgaaaatcg agttgcccgc
    30301 atctacggtt gtgccggaga ggtaataccc cagattttgg cttttcgcac aataaacggt
    30361 aagaggaatt ggcactgaac cagggtagtc cggcagagta acggtgacat cacgagcaga
    30421 aacatcgcag ccgccagtag gcaccaccac atcattattg gcgtaaatat tccacacaaa
    30481 ctgaaaatca tcgctgttat agttgttggt ctgtcgcaaa ataagcacgg caattaatga
    30541 gccagcttta atcgccaccc cgcccgcact gctcacaggc gtcaaataaa gcgccaccgg
    30601 ccacggctta tccgttctcg aattataaac aacgcgcggc gtttcgctgg tggtaggaaa
    30661 tggatcgcta ctgccactat attttacggt cccggaaaaa ttagataaca cgctgccata
    30721 agccgagcct cgttgcagtg tgacatagtc tgtaatggtt tccggataat cgttatggca
    30781 aaagatttgc gtcgaaagat ccacgaccag gttttgcccc acattcacgg cgggcgcaag
    30841 gtttacataa acattggcgc tgccaccgcc aatagggata gcggtaccat tggcggtttt
    30901 acaggcgaat gaccaggcat ttaccgacca gcccatcagc agtacagcaa acagggtaat
    30961 aactcgtttc attacaatca tctcttcggg ttcagctgta ggtataggtg atgctaatca
    31021 ctgcctgaat ggttccctga gtggctccgc catttaccgt caatgctctg acctgtaacg
    31081 ggaagtgcgc tgattgtgag gaatcatcca cctgaactgt tttggttgcg ccagtattca
    31141 atgtgttgcc actgtcatcc tgtagctcta actggatgtt ttgcgcggtc ccctggtttt
    31201 tataatatcc ggtactgtcg gctgccccgc tgaagctggc ggtgaccctc gacgttccca
    31261 ccggacaatt agtcaactca agcgcaacat catgccaggc cgatgccgcc ccggcagaca
    31321 tcagactgaa agaataaaga tcgccgagat caaccgtggc attggtggtg gaaaccgtac
    31381 acggtttggc gacgacctta ccgttcaccg tgatggtgac atcggctgcc tgtatcgtcg
    31441 cacttgcgag cgccaatatt gccgccaata aatacccacg tttgcgccat ttcatgagca
    31501 tctccagtta ctgatattca agagtgaagg tagccgtggc attgatatgc cccgcagtga
    31561 caggcacctg tgtcgccatt agccgggcgt aaaaattcaa cgtatttggt ttacccggcg
    31621 tcagggtcgt ccacgaaatc gcggacgatg gagcattaag gggtatttga ttttgctgct
    31681 cattcagaag ctgtattccc agtcccgaag ccgctgacac cgtattttca agtgcaagca
    31741 gattggcatt gtggctatct gcaacgccgg taaacccaac ttttacggca gaaacggcat
    31801 taccacaggg tgacagcaaa atacgaaatg gaacaacagg agtcgtcgcg ccaatgttgt
    31861 taaattgctt cgccgcgttt tccatcagat caacagtaaa attggttgat tcagcggcca
    31921 cactacagcc gttatctctg acatagccgc ggatagtaat cgtgctatcc gcagccaaag
    31981 cgtgactcac cgccagccac aaaaaagcgc acagaagata aaaaggtttg tttctcatca
    32041 cgccccctta acgacattca gctgatagct gggttaataa ctgctgctga ctctctggtg
    32101 gcagttgata attggcgaca cagtgagcat tttcctcttc tccccatttc acctgaactt
    32161 ttcccgctaa aggcattccg ctgaggtaaa cctgaccatt atccgcaaca atgccgctac
    32221 tctggctact ctctgatgtc accatcgccc caaacggcag cggcttatta ttgtgggtta
    32281 gcgtcatgag cagttttatc ccaacgcgcg ctttaaactc tgctcgcacg atcgccccac
    32341 gagtgggaac aacgttagcg accgcgttat ctaaatcgac gttatcagcc agggtattgg
    32401 tatccagcgc cactctgttt tccctatatt cagtggcata aggcagcacg gcataaccgc
    32461 gccagtcggt acgcaccccc gtctggtttt cgacttttgc atcttttgcg ccaggcgctt
    32521 taacaagcac caccgtatcg tttaacggct gccccagcgt tacgccattg gcatgagcca
    32581 ataccccacc gctgactccg taatagagct gcttaatatc atcgctatgg ctgtaaccga
    32641 tattggcatt gccgtaacca ccgcgataat tcagcgtggc gtagcctgtg cttccgctat
    32701 taccatcgcc tcccccggca tagccggttt gcacgctata gctgaggttg ttgtcttcca
    32761 gcaacgtacc gtatacacca gccagattgg tcatccgacc gttgagatcg tgtgacatgc
    32821 tgtagctggc actggcatgt cgccactgag atttactgtc agaacgcagc cagtggctga
    32881 aaggaatatt gacgttaagc gctaacatct gatcacgtcc tttttgccag gcgtttttcg
    32941 tcaggctata gctgagcgtc cagttgatat cttcgaacgc agtatttaat ccagcctgga
    33001 attgctcatc gacattactc gttccccaat aagtttgatg gctaccactc aaatacagtg
    33061 ttgatgtgcg cccgagttgc tgagtaacgg tgagctgtaa tttcccgcgt ttgttataag
    33121 cgaggttgta atagtcggtg aatttcggct taacctgaat aactccatcc tgtgtttcga
    33181 tgttgtagcc attcattcga ctgtatgttg tatcagcgaa attaaaatat ccgctggtcg
    33241 aataacggta acccactaac tgaatattcg tgcctgattc attgagcgat ttgttataga
    33301 gaaaacgcac cgattgtccg tcatgctgac tgtcatcggg aagtgtggaa ttagcctgcg
    33361 tcatatccac agacagagcg cccagtgccc ccatgttttt cccgataccg aaattaaaag
    33421 cacgataacg atccgccagt tgcgttccac catatattgt ccagccagcc ggaaggccgt
    33481 ggagtaatgt actctggaaa aagcggggtt tttcctgctg cgcatttcca ctacggtatt
    33541 ctcctgccgt aatggaataa cgagtatgcc cttcacgttg caaaagcggg actgacgaat
    33601 agggtacggt aaaaatctgc gtgctgccgt cagcctcttt gatcgttacc tgcaagtcac
    33661 cactattacc tgcggcatag atatcgttga tggtaaaagg ccccggtggc accgtactat
    33721 tataaatgtc atacccattt tgtttaatag tgacctgtgc agtaccacga gcaataccgt
    33781 ggatcaccgg ggcaaatcct ctttggctat cgggtaacat attgtcatct gaggccaatt
    33841 gtgcgccgcg aaagttaata ccatcgaaaa tatcgccctg agtataacca tcacccagcg
    33901 tcagccggga acgtaacggt attatgtctc gctcaagcca ggtattgata tgctgccatt
    33961 tatttttgct acctgatgat ctgtcgctac tgttataact ccaggtggta ttgtcgcgta
    34021 aacgccacgc accaatattt aacccactct gtaggtttaa atatgcataa tggctgttac
    34081 ccccaatccg attctgtaca ctatttccgc tgaaattata attgagcaat ccggcattaa
    34141 taccgggatc ccataactca ggaggaatat aaccacgcgc gcgattactc ataaatgcct
    34201 gagggatcgt caggttcagt cgctgctgac caacatccag atgcgcagta gcgtcctgga
    34261 ccattgtggt taatggcaca caggcatcat ccgccagcag attcataccg gcgacagaag
    34321 ccgtattcag ccccatactg gcgagttgcg cgcgtgtcag gcagggaaca atcccttgtt
    34381 cactgtcgcc cgtattaaat gtgacatcac gcgttgccat ataaccatta ttcaaataga
    34441 tatcgacgcg atacgtccct ggcggtaatt cttgcccatt ttcaaaacgc gataaatcgg
    34501 ccacagcctg gggatcatcc gctaaaaaac gcgggttaaa atagagttcg gcagatgaca
    34561 aaggtgcctg tgcggcaaaa gcacaggcaa caaagagccg gacaaaaaaa ccagccaaac
    34621 gatgcttacg aatatgcaag cattgtgtgt ttcgctggta aagtcttaaa ttcagatatg
    34681 acattattcc tccggtttaa agtcacccgg cagtcaattc tttttattca ggcgaaaaat
    34741 tccccctgcg ttattccatt acgcccgtca ttttgggggt aagtgcacca taatcattta
    34801 ttgttcggta agtaatattg cttcccgcat cagaaggcaa tttaaccgtg ctttcgccca
    34861 ttggaggcac caaggcattt tcaagaaccc gggttccggc attcaactct gttaccgtca
    34921 ggtaataggg tgtcgggtta atcagcgtca gagaattcgc gctacgacga aatcttaatt
    34981 tttctgcggc ctgatcgggt ggcaacgcta atttagccgg gcgatagtac agtttaatgc
    35041 ggctgataat tgcgagctgt agcgtattct cagtcaattt tgatttatcc attgacggaa
    35101 tcgctttaac attcatccag aataaacttt cccgatcctg tggcaattgg ttatttgttg
    35161 catcaagaat acgcaaggta ttctcttttt ttcccttcat cgcaaacaga ggaggcgtca
    35221 cgataaaacg accatccttt acaccatcgg cattttccac ccatgattga attaaatagg
    35281 tactattttc atcattattt gtcacggcaa gttgcacttg tttttgccct gccggataaa
    35341 ttacgcgagt cgcacctaag gccactcccg cttcagcgcg tccggcaacc atcattgcca
    35401 taaacatcag gatacctgcc agcaagcaga atgttatttc ctgcgatttc cttacattga
    35461 cgtttttatt actcactgtc ctgttcctgt tatcacatta tctgctgaac aattactgat
    35521 aggttaaaga gaaccaggcc tgggcattgg cgatgccgcc agtaacccga cgcccggtag
    35581 cacgatattt ggcgatgaaa tgtagcgaag tagagcctga gtaaagccgt ttccagtttg
    35641 ctggaggacg attaatcggt acgaggtttc cttcatcatc aaacaacgct acgccaatat
    35701 tggtggctat ccctggcccc tctcccacgg aaagcacatc cggattttta ccatccgcga
    35761 caccgtgaaa cgccaccccc acacgttcac tcaccaccgt gctacattcc cgtaaatgaa
    35821 taacaaaagg caccggtgcg ctatcttccc ctgccgcatg aaaccggtta ctgctgattt
    35881 gccccatatt gaccgtcatt tgtttatcac cggcttcaat ccggcaagtt tccgcaataa
    35941 tgacgccctg aaattgcata tttccgccgg gcagcgtggt attccattta tttccggcca
    36001 gagcaaacat tggcagcaac gacgctaaca gaaataatct cttccttttc atcgttattt
    36061 ttatcgcaca agggtgggca tccctgcccg taatgacgtc cttgaacctg ggtaggttat
    36121 tgatactgaa ccttgaaggt tgcatccgca ttagctgcac ccggggttgc ctcgccgatt
    36181 gcataataac gcgcctggaa cggaatggtg ttggtaccgt tattcagggt tgtttgtgca
    36241 ctaaatgtcg caccgtccag cgtcagagca ttgcctgttc tgtccaggat ctgcacgcca
    36301 acgtttgttg cactacctgc agcagaactc tgcagagcca gtacatcagt acgcgtagca
    36361 tcaattgccg tacctaagaa ggcaacagcg gcttttgtgg caacagtggt atcgcaatca
    36421 ttcaactgaa tgttaaaacc aacggcagag ctggttgctc cagcctgctt caggctagcg
    36481 gtacgaacct gtcccaactg aacggtttga tcaacagagc ctgcatcaac tgcgcaagcg
    36541 gcgttaacaa cttccccttt aaagtgaacg gtcccaccat ttaccgtcgt agtatcggcc
    36601 agagccgctg cggaactgag ggacagagcc gacagaacaa cgattgccag agttttaatt
    36661 ttcatgctgc tttcctttaa aaaaactatt tctaaatcga catgggcagt cgttctgtac
    36721 actttgtttt gtcaacgagt ttgactgcca acactgcaca gttttccccc aaaagatgaa
    36781 acatttgggg ccattttgac tcatagagga aagcatcgcg cacaaacttt ttcagtttat
    36841 ttgttggctt aatattctat tgttatcttt atttatagat ttttatattg catgaggtgg
    36901 tttttggaga gaagaatgag gaagatgtcg agccacagaa a
//
LOCUS       DAZGJW010000031        36789 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZGJW010000031 DAZGJW010000000
VERSION     DAZGJW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.031"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(60..1652)
                     /gene="ybiT"
                     /locus_tag="ACHKIK_001622"
     CDS             complement(60..1652)
                     /gene="ybiT"
                     /locus_tag="ACHKIK_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO9082815.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHKIK_001623"
     CDS             1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHKIK_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO9082816.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2850..3968)
                     /gene="ybiR"
                     /locus_tag="ACHKIK_001624"
     CDS             complement(2850..3968)
                     /gene="ybiR"
                     /locus_tag="ACHKIK_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO9082817.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHKIK_001625"
     CDS             complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHKIK_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO9082818.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4618..4746
                     /gene="mntS"
                     /locus_tag="ACHKIK_001626"
     CDS             4618..4746
                     /gene="mntS"
                     /locus_tag="ACHKIK_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO9082819.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5018..6601
                     /gene="opgE"
                     /locus_tag="ACHKIK_001627"
     CDS             5018..6601
                     /gene="opgE"
                     /locus_tag="ACHKIK_001627"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO9082820.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHKIK_001628"
     CDS             complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHKIK_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO9082821.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7218..7283)
                     /locus_tag="ACHKIK_001629"
     CDS             complement(7218..7283)
                     /locus_tag="ACHKIK_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9082822.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7518..8405
                     /gene="rhtA"
                     /locus_tag="ACHKIK_001630"
     CDS             7518..8405
                     /gene="rhtA"
                     /locus_tag="ACHKIK_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO9082823.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            8704..9207
                     /gene="dps"
                     /locus_tag="ACHKIK_001631"
                     /gene_synonym="pexB"
     CDS             8704..9207
                     /gene="dps"
                     /locus_tag="ACHKIK_001631"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO9082824.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            9611..10357
                     /gene="glnH"
                     /locus_tag="ACHKIK_001632"
     CDS             9611..10357
                     /gene="glnH"
                     /locus_tag="ACHKIK_001632"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO9082825.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            10496..11155
                     /gene="glnP"
                     /locus_tag="ACHKIK_001633"
     CDS             10496..11155
                     /gene="glnP"
                     /locus_tag="ACHKIK_001633"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO9082826.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVLYLIITLVLSFILRRLERRMKI
                     L"
     gene            11152..11874
                     /gene="glnQ"
                     /locus_tag="ACHKIK_001634"
     CDS             11152..11874
                     /gene="glnQ"
                     /locus_tag="ACHKIK_001634"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO9082827.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            11991..14216
                     /gene="ybiO"
                     /locus_tag="ACHKIK_001635"
     CDS             11991..14216
                     /gene="ybiO"
                     /locus_tag="ACHKIK_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO9082828.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPPPPGEPT
                     L"
     gene            complement(14213..15139)
                     /gene="rlmF"
                     /locus_tag="ACHKIK_001636"
     CDS             complement(14213..15139)
                     /gene="rlmF"
                     /locus_tag="ACHKIK_001636"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO9082829.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            15415..15660
                     /gene="mcbA"
                     /locus_tag="ACHKIK_001637"
                     /pseudo
     CDS             15415..15660
                     /gene="mcbA"
                     /locus_tag="ACHKIK_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
     gene            15938..18220
                     /gene="fiu"
                     /locus_tag="ACHKIK_001638"
     CDS             15938..18220
                     /gene="fiu"
                     /locus_tag="ACHKIK_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO9082830.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            18262..18939
                     /gene="ybiX"
                     /locus_tag="ACHKIK_001639"
     CDS             18262..18939
                     /gene="ybiX"
                     /locus_tag="ACHKIK_001639"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO9082831.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            19013..19279
                     /gene="ybiI"
                     /locus_tag="ACHKIK_001640"
     CDS             19013..19279
                     /gene="ybiI"
                     /locus_tag="ACHKIK_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO9082832.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKGLQKPAYTGYSRRGSKDSQLR"
     gene            19544..19804
                     /gene="ybiJ"
                     /locus_tag="ACHKIK_001641"
     CDS             19544..19804
                     /gene="ybiJ"
                     /locus_tag="ACHKIK_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO9082833.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(20036..20518)
                     /gene="ybiA"
                     /locus_tag="ACHKIK_001642"
     CDS             complement(20036..20518)
                     /gene="ybiA"
                     /locus_tag="ACHKIK_001642"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFO9082834.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            20638..22788
                     /gene="dinG"
                     /locus_tag="ACHKIK_001643"
     CDS             20638..22788
                     /gene="dinG"
                     /locus_tag="ACHKIK_001643"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO9082835.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            22816..23778
                     /gene="ybiB"
                     /locus_tag="ACHKIK_001644"
     CDS             22816..23778
                     /gene="ybiB"
                     /locus_tag="ACHKIK_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO9082836.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNTAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            23919..25004
                     /gene="hcxB"
                     /locus_tag="ACHKIK_001645"
     CDS             23919..25004
                     /gene="hcxB"
                     /locus_tag="ACHKIK_001645"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO9082837.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKESGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHKHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGALLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            25071..25130
                     /gene="ybiE"
                     /locus_tag="ACHKIK_001646"
     CDS             25071..25130
                     /gene="ybiE"
                     /locus_tag="ACHKIK_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO9082838.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(25235..26599)
                     /gene="rhlE"
                     /locus_tag="ACHKIK_001647"
     CDS             complement(25235..26599)
                     /gene="rhlE"
                     /locus_tag="ACHKIK_001647"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO9082839.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGDGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            26828..27499
                     /gene="cecR"
                     /locus_tag="ACHKIK_001648"
     CDS             26828..27499
                     /gene="cecR"
                     /locus_tag="ACHKIK_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO9082840.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            27502..28497
                     /gene="hlyD"
                     /locus_tag="ACHKIK_001649"
     CDS             27502..28497
                     /gene="hlyD"
                     /locus_tag="ACHKIK_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO9082841.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            28490..30226
                     /gene="ybhF"
                     /locus_tag="ACHKIK_001650"
     CDS             28490..30226
                     /gene="ybhF"
                     /locus_tag="ACHKIK_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO9082842.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            30219..31352
                     /gene="ybhS"
                     /locus_tag="ACHKIK_001651"
     CDS             30219..31352
                     /gene="ybhS"
                     /locus_tag="ACHKIK_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9082843.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            31363..32469
                     /gene="ybhR"
                     /locus_tag="ACHKIK_001652"
     CDS             31363..32469
                     /gene="ybhR"
                     /locus_tag="ACHKIK_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9082844.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(32431..32841)
                     /gene="ybhQ"
                     /locus_tag="ACHKIK_001653"
     CDS             complement(32431..32841)
                     /gene="ybhQ"
                     /locus_tag="ACHKIK_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO9082845.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            32974..33735
                     /gene="ybhP"
                     /locus_tag="ACHKIK_001654"
     CDS             32974..33735
                     /gene="ybhP"
                     /locus_tag="ACHKIK_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO9082846.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPKGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            33732..34973
                     /gene="clsB"
                     /locus_tag="ACHKIK_001655"
     CDS             33732..34973
                     /gene="clsB"
                     /locus_tag="ACHKIK_001655"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO9082847.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            34973..35929
                     /gene="ybhN"
                     /locus_tag="ACHKIK_001656"
     CDS             34973..35929
                     /gene="ybhN"
                     /locus_tag="ACHKIK_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO9082848.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPVGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(35965..36678)
                     /gene="ybhM"
                     /locus_tag="ACHKIK_001657"
     CDS             complement(35965..36678)
                     /gene="ybhM"
                     /locus_tag="ACHKIK_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO9082849.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
BASE COUNT         8850 a         9616 c         9512 g         8811 t
ORIGIN      
        1 attttgcatt tgtcatcagc cgccgtcacc ccgacagtat cggggtgacg gataatcact
       61 tactcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt
      121 tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg gtcgtgtgaa
      181 acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc gatggattcc
      241 atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac
      301 ttaccaaaca gcatccgccc tttttcccca ccggaaagca ctttagctgg ctttttgatg
      361 tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct
      421 tcctgcttcc actgactcat ccattcgaac acggtcagat cattttcaaa ctcatattcg
      481 tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc attttacggt gccgctgtcc
      541 ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc
      601 agtaccgcaa gtttttcacc gacctccagc agcaggttga gatttttaaa cagtggaccg
      661 ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag tttcttatcc
      721 tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt
      781 ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag
      841 cggctaacga aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt atcggccagc
      901 agacgttcac gcgcctgggt cgccgccgtc atgtactcat cgtagttacc cggataaacg
      961 cgcagctcgc cgtaatccag atccgccatg tgggtacaga ccatgttaag gaagtgacgg
     1021 tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga
     1081 atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatatc cggatcagca
     1141 aacagcgcct gcgccagaag cacacgcagc ttccagccag gagcaacttc actcatcggg
     1201 ccgtagtgct gttccactgg aattcccacg ccaagcaaca attcaccggc gcgagcttcc
     1261 gcagagtaac cgtccatttc gccgtattta acttccagat cggccacttt atagccgtct
     1321 tcctcactca tttccggcaa agcatagatg cggtcgcgct cctgcttcac ttcccacaac
     1381 tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc
     1441 tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccaa agtcggctct
     1501 aagtcgccgc cgaggatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg
     1561 ccgtaacggt tgccgccgcc aaatttgacg gaaatgtttt caaacaacgg cttactgccg
     1621 aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa
     1681 accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc
     1741 aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa
     1801 cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat
     1861 ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg
     1921 cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt
     1981 tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc
     2041 cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt
     2101 cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt
     2161 tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac
     2221 caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa
     2281 ctggaccacc aaagttgagc gtaagaaagc aggcccgacc tggacgccga ccgccaaaat
     2341 gcacgcagag taccgcgctg cgggcgaacc gcttccggct gtcgttccgg caggtccgga
     2401 taacccgatg ggtctgtatg cactctacat cggtcgcctg tatgctatcc atggcaccaa
     2461 cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga
     2521 catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc
     2581 ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc
     2641 taccaccgaa gcccagtttg aaggtcagga aattgtgcca attaccctga cgaagagcgt
     2701 gcaaacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag cgattaaaaa
     2761 ccgctccggg atgccggttc gtctgaatta atcttcaaaa cttaaagcaa aaggcggact
     2821 cataatccgc cttttttatt tgccagaccc tagttggccg ggagtataac taacaaaaca
     2881 tatcccacca acgccgccca caacagcatc ggtattgaat agagatggaa acgccaccag
     2941 atgcggcgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc gggtaataac
     3001 ccaaagccac ctacgtttac cgcccatacc agtaataaag acggcggcac atagttcagc
     3061 aacaatatgg tactcggcac gttactgatc acctgcgata aaccgattgc cgttaaccat
     3121 aacccgggtt cagatagatg actcacgtta cccaacacgc cttgcaacgc tggaagctgg
     3181 gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcagcgt ccagtccaca
     3241 ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg ccacaatcac cagtccccac
     3301 agctcttgtt tgaactccag cgccgtcaga aagacgatat acagccccag acaactccac
     3361 accagccgcg gtttccactc cggtgtttgc acccccgtat ggtattgcag cgcctttcca
     3421 gggaaacaac accagcacag gagcaggagc gtcagcatca ttgcgccagc cagcggtgcc
     3481 atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag aatattttgc
     3541 gggttgccaa ttggcgtcag tagcgaacca gcgttgactg ccagcgcctc aaaaataatc
     3601 agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg aacaacaata
     3661 aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag caccataaac
     3721 atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag cacatcaaaa
     3781 taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt gatgatggtg
     3841 tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa gcttaatccg
     3901 ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg taaaaaaggc
     3961 aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa agcgtcgagc gtctcttcac
     4021 taacatggtg ctccatgcct tccgcgtcgc gacgggcgat ttccggactg acgcccaaca
     4081 ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc agcttctctc
     4141 cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc atggttgcca
     4201 gccgcttaag cattttagcc accgtcggtt gcgaaactcc cagacgagca gccatgtcca
     4261 cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaacg taatcatcaa
     4321 taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc ttcaacgtgc tcttccacgt
     4381 tcactaactg cgtcactttt tttgctgttg gcgtacctgc gcgacgactc attgtgcttc
     4441 ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta aaccagagtt
     4501 gcgaaggtac aaaaaattaa cgttttagca atagctatat aatatagcct gtgctatatc
     4561 tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta gcaagtcagg aggtcttatg
     4621 aatgagttca agaggtgtat gcgcgtgttt agtcattctc cctttaaagt acggttaatg
     4681 ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaagataa accttccgat
     4741 aaatagcggc gtcgcggtac gccgcttcac tcctgctttc atgcaggcat aacgcgtttt
     4801 ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc ttcgcaaaag atttgttcgt
     4861 cagtagttga cctgaacggc ggctcgctct atcttcttgc agccctgcgt atattgcggc
     4921 tcgcggatgc ggaccccttt cccctcttca cgcactcttg caggtattga cccttgacgc
     4981 cagggtaagc acatggcgtt tgttacgata gtggcatatg aatttaaccc tcaaagaatc
     5041 gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt tacagtcgct
     5101 attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg cgtttacggc
     5161 tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg tcggtatcag
     5221 ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc ctaacttcaa
     5281 tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc tgacgatttt
     5341 tccgtggtac agctacatgg tcggcatatt tatttttgtg ctcggcgtaa tagcaatcag
     5401 gcgaaaaaaa gagagtgaaa aagcgcgctg gaataccttc gacagcctgt gtctggtatt
     5461 cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggtg gcgtatttaa
     5521 actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg tcaataataa
     5581 cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag atacctggac
     5641 ggtcactgcc gttaagccga agtatcagac ctatgtggtg gtgatcggtg aaagcgcgcg
     5701 tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg ccagcagcgt
     5761 taacggtttg atatttgctg actacattgc cgccagtggc tccacgcaga aatcgcttgg
     5821 cttaacgctc aatcgcgtag tcgatggcaa accacagttt caggataact ttgtcaccct
     5881 ggcaaatcgc gcgggcttcc agacctggtg gttttccaac cagggccaaa tcggcgaata
     5941 cgatactgct atcgccagca tcgccaaacg tgcagatgaa gtgtacttcc tgaaagaagg
     6001 taattttgaa gcagataaaa acacgaaaga cgaagcgtta ctggatatga ccgctcaagt
     6061 gctggcgcaa gagcactcgc aaccgcagct gattgttctg catctgatgg gctcgcatcc
     6121 gcaggcctgc gacaggacac aaggcaaata cgaaaccttt gtgcaatcga aagaaacgtc
     6181 gtgctatctc tataccatga cgcaaacgga cgatttactg cgcaagctgt acgatcagtt
     6241 acgcaacagc ggcagcagct tctctctggt ttacttttct gaccacggtc tggcctttaa
     6301 agagcgcggc aaagacgtgc aataccttgc ccatgatgat aaatatcagc aaaatttcca
     6361 ggtgcctttt atggtcattt ccagcgacga taaagcgcat cgggtgatta aagcccgccg
     6421 ctcagccaat gacttcttag gctttttctc gcagtggacg ggaattaaag cgaaggaaat
     6481 aaatatcaaa tacccgttta tatctgagaa gaaagccggg ccgatataca tcaccaactt
     6541 ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc cgaaacctta
     6601 aaacaaaaaa tccgccccga gaggcggatt ttttatatca ccaaagtgat tagaagcggt
     6661 aaccaacacc ggcaatccag gtacctacgt caacgctacg aatacggctc tgctcgtaag
     6721 agaagtccag agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac
     6781 cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattccgt ggtctggaat ttaccataac
     6841 ccacccccac tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag
     6901 tgatgccgta gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg
     6961 tgtaagtgaa agagccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca
     7021 ggttgaaacc gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag
     7081 ttacggtaga agtcgcagct acggaagtac ctgcggtgaa agccagaact gcggccagtg
     7141 ctgaaagaca tgcaattttt ttcataacca cctcaaatgt gattcaaata agtcctaagt
     7201 tttaaatata tcaaaaatta atgggaaact cttcgcgatt tgtgatgtct aacgggccat
     7261 ttcatgtaac agaacgtttc catacaccgc tatccatcta aatttaaatc actttttcag
     7321 agaactgcgt aagtattacg catgttttcc ctgtcattca tccagattat tcctaatcac
     7381 cagactaatg attccatcaa tcctggcgca ttttagtcaa aacgggggaa aattttttca
     7441 acaaatgctc aaccatcatt gggtatatcc agtacactcc acgctttact taagtctaga
     7501 tatttgtggg agaaaggatg cctggttcat tacgtaaaat gccggtctgg ttaccaataa
     7561 tcatattgct cgttgccatg gcgtctattc agggtggagc ctcgttagct aagtcacttt
     7621 ttcctctggt gggcgcaccg ggtgtcactg cgctgcgtct ggcattagga acgctgatcc
     7681 tcatcgcgtt ctttaagcca tggcgactgc gctttgccaa agagcaacgg ttaccgctgt
     7741 tgttttacgg cgtttcgctg ggtgggatga attatctttt ttatctttct attcagacag
     7801 taccgctggg tattgcggtg gcgctggagt tcaccggacc actggcggtg gcgctgttct
     7861 cttctcgtcg cccggtagat ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc
     7921 tgctaccgct ggggcaagac gtttcccatg tcgatttaac cggctgtgcg ctggcactgg
     7981 gggccggggc ttgttgggct atttacattt taagtgggca acgcgcagga gcggaacatg
     8041 gccctgcgac ggtggcaatt ggttcgttga ttgcagcgtt aattttcgtg ccaattggag
     8101 cgcttcaggc tggtgaagca ctctggcact ggtcggttat tccattgggt ctggctgtcg
     8161 ctattctctc gaccgctctg ccttattcgc tggaaatgat tgccctcacc cgtttgccaa
     8221 cacggacatt tggtacgctg atgagcatgg aaccggcgct ggctgccgtt tccgggatga
     8281 ttttcctcgg agaaacactg acacccatac agctgctggc gctcggcgct atcatcgccg
     8341 cttcaatggg gtctacgctg acagtacgca aagagagcaa aataaaagaa ttagacatta
     8401 attaaattta catttctgca tggttatgca taaccatgca gaatttctcg ctacttttcc
     8461 tctacaccgt ctttatatat cgaattatgc aaaagcatat ttattccgaa aattcctggc
     8521 gagcagataa ataagaattg ttcttatcaa tatatctaac tcattgaatc tttattagtt
     8581 ttgtttttca cgcttgttac cactattagt gtgataggaa cagccagaat agcggaacac
     8641 atagccggtg ctatacttag tctcgttaat tactgggaca taacatcaag aggatatgaa
     8701 attatgagta ccgctaaatt agttaaatca aaagcgacca atctgcttta tacccgcaac
     8761 gatgtctccg acagcgagaa aaaagcaaca gtagagttgc tgaatcgcca ggttatccag
     8821 tttattgatc tttctttgat taccaaacaa gcgcactgga acatgcgcgg cgctaacttc
     8881 attgccgtac atgaaatgct ggatggcttc cgcaccgcac tgatcgatca tctggatacc
     8941 atggcagaac gtgcagtgca gctgggcggt gtagctctgg ggaccactca agttatcaac
     9001 agcaaaaccc cgctgaaaag ttacccgctg gacatccaca acgttcagga tcacctgaaa
     9061 gaactggctg accgttacgc aatcgtcgct aatgacgtac gcaaagcgat tggcgaagcg
     9121 aaagatgacg acaccgcaga tatcctgacc gccgcgtctc gcgacctgga taaattcctg
     9181 tggtttatcg agtctaacat cgaataaatc catcgctgat ggtgcagaac tttagtaccc
     9241 gataaaagcg gcttcctgac aggaggccgt tttgttttgc agcccacctc aacgcactta
     9301 tttagtgcat ccatctgcta tctccagctg attaagtaaa ttttttgtat ccacatcatc
     9361 acacaatcgt tacataaaga ttgttttttc atcaggtttt acgctaaata atcactgtgt
     9421 tgagtgcaca attttagcgc accagattgg tgccccagaa tggtgcatct tcagggtatt
     9481 gccctataaa tcgtgcatca cgtttttgcc gcatctcgaa aaatcaatga gttgcaaaac
     9541 tggcacgatt ttttcatata tgtgaatgtc acgcagggga tcgtcccgtg gatagaaaaa
     9601 aggaaatgct atgaagtctg tattaaaagt ttcactggct gcactgaccc tggcttttgc
     9661 ggtttcttct catgccgcgg ataaaaaatt agttgtcgcg acggataccg ccttcgttcc
     9721 gtttgaattt aaacagggcg ataaatatgt gggctttgac gttgatctgt gggctgccat
     9781 cgctaaagag ctgaagctgg attacgaact gaagccgatg gatttcagtg ggatcattcc
     9841 ggcactgcaa accaaaaacg tcgatctggc gctggcgggc attaccatca ccgacgagcg
     9901 taaaaaagcg atcgatttct ctgacggcta ctacaaaagc ggcctgttag tgatggtgaa
     9961 agctaacaat aacgatgtga aaagcgtgaa agatctcgac gggaaagtgg ttgctgtgaa
    10021 gagcggtact ggctccgttg attacgcgaa agcaaacatc aaaactaaag atctgcgtca
    10081 gttcccgaac atcgataacg cctatatgga actgggcacc aaccgcgcag acgccgttct
    10141 gcacgatacg ccaaacattc tgtacttcat caaaaccgcc ggtaacggtc agttcaaagc
    10201 ggtaggtgac tctctggaag cgcagcaata cggtattgcg ttcccgaaag gtagcgacga
    10261 gctgcgtgac aaagtcaacg gcgcgttgaa aaccctgcgc gagaacggaa cttacaacga
    10321 aatctacaaa aaatggttcg gtactgaacc gaaataataa cgctacacct gtaaaacgca
    10381 ctggcagttc cctctcccct ctggggagag gattagggtg aggggcgcaa acccgctccg
    10441 gggccattaa ttaccctgaa tttgattatt tacaccacgg taacaggaac aacatatgca
    10501 gtttgactgg agtgccatct ggcctgccat tccgcttctg attgaaggtg ccaaaatgac
    10561 cctgtggatt tcggtcctcg gtctggcagg cggtctggta atcggattgc tggcaggttt
    10621 tgcacgcacc ttcggtggtt ggatagccaa ccacgtcgcg ctggtcttta ttgaagtgat
    10681 ccgcggcaca cctatcgtcg tccaggtgat gtttatctat ttcgccctgc cgatggcgtt
    10741 taacgactta cgcatcgacc catttactgc ggcggtggtc accatcatga tcaactccgg
    10801 cgcgtacatt gcggaaatca cgcgtggtgc ggtgctgtct atccacaaag gttttcgtga
    10861 agcaggactg gcgctcggtc tttcacgttg ggaaaccatt cgctacgtca ttttaccgct
    10921 ggcactgcgt cgtatgctgc cgccgctggg taaccagtgg atcatcagca ttaaagacac
    10981 ctcgctgttt attgtgatcg gcgtggcgga actgacccgt caggggcaag aaattattgc
    11041 cggtaacttc cgcgcccttg agatctggag cgccgtggcg gtgctctatc tgattattac
    11101 cctggtgctg agctttattc tgcgtcgtct ggaaagaagg atgaaaatcc tgtgattgaa
    11161 tttaaaaacg tctccaagca ctttggccca acccaggtgc tgcataatat cgatttgaac
    11221 attgcccagg gcgaagtcgt ggtgattatc gggccgtccg gttccggtaa atcgaccctg
    11281 ctgcgctgca tcaacaaact ggaagaaatc acctccggcg atctgattgt cgatggcctg
    11341 aaggttaacg atccgaaagt tgacgagcgc ctgattcgcc aggaagcagg tatggtgttc
    11401 cagcagtttt acctcttccc gcatctgacg gcgctggaaa acgtcatgtt tggtccgcta
    11461 cgcgtgcgtg gcgcgaacaa agaagaggcg gaaaaactgg cgcgtgagct gctggcgaaa
    11521 gtcggtctgg cagaacgtgc acatcactac ccttccgaac tttctggtgg tcaacagcag
    11581 cgtgtggcga ttgcccgcgc gctggcggtg aagccgaaaa tgatgctgtt tgatgaaccg
    11641 acttccgctc ttgacccgga actgcgccat gaagtgctga aggttatgca ggatctggct
    11701 gaagaaggga tgacgatggt gatcgtgacc cacgaaatcg gttttgccga gaaagtagct
    11761 tcgcggctga tctttatcga caaaggccgg attgcggaag atggcgatcc gcaggtattg
    11821 atcaagaacc cgccgagcca gcgcttgcag gaatttttgc agcacgtctc ttaacttctc
    11881 tgcctccctt tcacccgaaa gggaggcaca ccagattcct ctcatttaaa atcgcccctc
    11941 ccccagcatc tatacttatc tttttgctct gttttctcac tggaggagtc atgcggtgga
    12001 tcctgttcat cctcttctgc ctgctgggcg cacctgccca cgcggtatcc atacccggcg
    12061 ttacaaccac aacgacaacg gactcaacga ctgaaccggc cccggaaccg gatatcgaac
    12121 aaaaaaaagc ggcctatggc gcactggcgg atgtgctgga taacgacacc tcgcgtaaag
    12181 aattgatcga ccagttgcgt actgtcgccg cgacgccccc tgcggaaccg gtcccgaaga
    12241 tcgtgccgcc gacgctggtt gaagagcaaa ccgtgctgca aaaggtcacc gaagtcagcc
    12301 gccattatgg tgaagccctt tccgcccgct tcgggcaact ttatcgcaat atcaccggct
    12361 ccccgcataa gccatttaat ccacaaacct tcagcaatgc gctgagccat ttttcaatgt
    12421 tagcggtatt agtgtttggt ttttactggc tgattcgcct gtgcgcactg ccgctgtatc
    12481 gcaaaatggg tcagtgggcg aggcaaaaaa atcgtgagcg cagcaactgg ttgcagcttc
    12541 cggcgatgat tatcggggcg tttattatcg acctgctgtt actggccctg acattatttg
    12601 tcggccaggt attaagcgac aacctgaatg caggcagtcg caccatcgct ttccaacaaa
    12661 gtttgtttct caatgccttt gccctgattg aatttttcaa agccgtacta cgcctgattt
    12721 tttgcccaaa cgtggcggag ctgcgtccgt tcacgattca ggacgagagc gcccgttact
    12781 ggagtcgtcg cctgagctgg ttaagcagtt tgataggtta tggcctgatt gtggccgtgc
    12841 cgattatctc taatcaggtg aatgtacaga taggtgcgct ggcgaacgtc atcattatgc
    12901 tgtgcatgac cgtctgggcg ttgtacctga tctttcgtaa taaaaaagag attacccagc
    12961 atttgctcaa ctttgcggag cattcgctgg cctttttcag cctgtttatc cgcgcctttg
    13021 cgctggtgtg gcactggctg gcaagcgcct attttatcgt gctgtttttc ttttcgttgt
    13081 tcgatccggg caacagcctg aaatttatga tgggtgcaac ggtgcgcagc ctggcgatta
    13141 ttggtatcgc agcgtttgtt tccggtatgt tttcccgctg gctggcgaaa accatcaccc
    13201 tctcgccaca tactcagcgt aactatccgg agctacaaaa acggctgaat ggctggcttt
    13261 cggcggcgct gaaaacggcg cgtattctga ccgtctgcgt ggcagtaatg ttgctgttga
    13321 gcgcatgggg attgttcgat ttctggaact ggctgcaaaa cggcgcgggg cagaaaaccg
    13381 tagatatcct gatccgcatc gcactcattc ttttcttctc ggcggttggc tggacggtgc
    13441 tcgccagttt gatcgaaaac cggctggctt cggatattca tggccgcccg ctacccagcg
    13501 cccgtacgcg taccctgctg acgctgtttc gtaacgcgct ggcggtgatt atcagtacca
    13561 tcaccatcat gattgtgttg tcggaaatcg gcgtcaatat cgcgccattg ctggcaggtg
    13621 ccggggcatt aggtctggct atctcgtttg gttcgcaaac gctggtgaaa gatatcatta
    13681 ccggggtgtt tattcagttt gaaaacggca tgaacactgg agatttggtg actatcgggc
    13741 cgttgaccgg cacagtggaa cggatgtcga ttcgctccgt gggcgtgcgc caggataccg
    13801 gggcgtatca catcattccg tggtcttcga taaccacctt tgctaacttc gtccgcggta
    13861 ttggttcggt agtggcaaac tacgatgttg atcgccatga agatgctgat aaagccaatc
    13921 aggcactgaa agatgcggta gcggaattga tggaaaacga agaaattcgc gggctgatta
    13981 ttggtgaacc gaattttgcc gggattgtcg gcttaagcaa taccgcgttt acactgcgtg
    14041 tttcgttcac cacgctgcca ctcaaacagt ggacggtacg ctttgccctc gacagccagg
    14101 tgaaaaaaca tttcgacctg gcgggcgttc gcgcgccagt gcagacttat caggtgctgc
    14161 ctgctccggg cgcgaccccg gctgaaccgc cgccgccggg ggagccaacg ctttaacgtt
    14221 ggcgattaac aaaacggcgg cgctgctcgt cgttcataaa ggtccaggca ataaagcgac
    14281 tctgcttttg cccctgggcc atctcttttt ttaccacctt caccgcgccc acgtcggtca
    14341 gagcacgata caacggcggt aagttttcac cacgagatac cagcgaggta aaccacatca
    14401 cctgcttcgc gaagcctttg ctctcttcaa tcatcttttt gataaaggcg acttcaccac
    14461 cttcacacca caactcctgt tgctgaccac caaagttcag tgcatcgtct ttgttcagcc
    14521 ccaggttacg gcgtttacgc tcactacctg cccgcgctgc agcggcggaa tcgtggaatg
    14581 gcgggttaca caaggtcgcg tcgtattgct cgtttttatg gatgatgccg ttaaaaattg
    14641 ccccactctc tttttgccga cgcagacgaa tggcgcggtt aagccccgga ttagcactga
    14701 taatcgcctg cgcactgctt aacgcctggc tgctggtttc actaccggta aaacgccagc
    14761 catattcatg tacgccaatc agcggataaa tacagttcgc accaacgccg atatccagaa
    14821 tgctggcatt cgctggaatt gttccgcttg cttcagccag taaatcggca aggtgatgaa
    14881 tataatccgc ccggcccggt actggtgggc agagaaaacc gtcggggata tcccagttcg
    14941 ctacggcgta aaaatgggcc agcaacgcct tattgagcgc cttcaccgcc agcggattgg
    15001 caaagtctac gctttgctcc ccggcgggtg taagcgtgag gaattgcctg agttcaggat
    15061 tgacctgaca aagcgtggcg agatcgtagc ggctgtgatg acggttgcgc ggatgcaacc
    15121 ccggtttctg ggcggacata gcattctcct ttaagcagcg gcgtaagata cccgcataac
    15181 gcgacagggt aaatatctgg ctgcgtttat cattccccaa aaccttaaca tggctatggt
    15241 ttatggcaat tcatcataaa aatcagcatt atgcatgtca ttgatttgtc ttattttgta
    15301 aaaatatatc gttcaaaaaa taagcacatc tctgtcaatt ttttgcgtca ttttcatcgc
    15361 ccaggcgacc tacacttatc aggcactacc cgttgtattt atgaggatgt cattatgaaa
    15421 aagtgcctca cactactgat tgccaccgtc ctgagcggaa tctctctcac ggcttatgcc
    15481 gcgcaaccga tgagtaacct ggacagcggt cagctacggc ccgccggtac cgtttcggca
    15541 actggcgcat caaacctaag cgatctggag gataaactgg cagaaaaagc gcgcgagcaa
    15601 ggcgcgaaag gttatgtcat taattccgca ggcggaaatg accagatgtt cggtactgca
    15661 ccatctacaa ataaccccac caacgtaact gcattcgcct ctggatgcag ttttcgcctg
    15721 ttttgtaatc aaaaaacatc aaatatgaac gcaatgtaaa taaatgtatt tctttttcgc
    15781 gcaatgggtg atagaaaatc gcgccaagtg ataatgctta tcaaaattat tatcacttgc
    15841 acgaacacta tcacgagatt aacagtggca tcgcatccgc agagaggctt tctcgtggca
    15901 gtgaaaattt caacatataa aaaaagtcac ctgcaaaatg gaaaacaatc gcaatttccc
    15961 tgccagacaa tttcattcgc tcacgttctt tgccggtctt tgtattggca tcacgcctgt
    16021 ggctcaggca ctcgccgccg aagggcaaac taacgcggat gacacgctgg ttgtcgaagc
    16081 atcaacgcct tcgctttatg cgccacaaca atctgccgat ccgaaattct cgcgtccggt
    16141 agcggatact acccgcacga tgacggtgat ttctgaacaa gtgattaaag atcagggcgc
    16201 aaccaacctt accgacgcgc tcaaaaacgt ccccggcgtg ggtgcgtttt ttgcgggtga
    16261 gaacggtaac tccaccactg gcgacgccat ttatatgcgc ggtgctgata cctctaacag
    16321 tatttatatt gatggcattc gcgatatcgg cagcgtctcg cgcgacacct tcaatactga
    16381 gcaggtcgaa gtgattaaag ggccttccgg caccgactac gggcgcagcg cgccgacggg
    16441 ctcgatcaat atgatcagca agcagccgcg caatgattcc ggcattgacg cctccgccag
    16501 tattggcagc gcctggttcc gccgcggcac gctggacgtc aatcaggtca ttggtgatac
    16561 cactgcggtg cgcctgaatg tgatgggcga aaaaacgcac gatgccggac gcgacaaagt
    16621 caaaaatgag cgttacggcg tcgccccttc tatcgctttt ggccttggta cagcgaatcg
    16681 tttgtatctt aattatctgc atgtcaccca gcacaacacg ccagacggcg gcattccgac
    16741 catcggtttg ccgggttatt ctgccccatc tgcgggaaca gcggctctga atcattccgg
    16801 aaaagttgat actcataact tttacggcac ggattccgat tacgacgatt cgaccaccga
    16861 caccgccacc atgcgttttg agcacgacat caacgataac accaccattc gcaatactac
    16921 ccgttggtcg cgcgtaaagc aggattacct gatgacggcg attatgggcg gggcgtcgaa
    16981 tattactcag cccaccagcg atgtgaatag ctggacctgg tcacgcacgg cgaataccaa
    17041 agatgtgagt aataaaattc tcaccaacca gaccaacctg acctcgacgt tctataccgg
    17101 ttctatcggt catgatgtca gtaccggcgt ggaatttacc cgtgaaacgc agactaacta
    17161 cggcgttaat ccggtgacgt tacccgcggt aaatatttat catcctgaca gcagcattca
    17221 tcccggcggc ctgacgcgca acggcgcaaa cgccaatggt cagacggata ccttcgcaat
    17281 ttacgccttc gatacgctgc aaatcacccg tgattttgag ctgaacggcg ggatccgtct
    17341 ggataattat catactgaat atgacagtgc caccgcctgc ggcggcagcg gacgcggtgc
    17401 catcacctgc ccaactggtg tggcaaaagg ttctccggtc accaccgtcg acaccgccaa
    17461 gtcgggcaat ctggtgaact ggaaagccgg ggcgctgtat cacctgacgg aaaacggtaa
    17521 tgtctatatt aactatgccg tttcccagca gcctccgggc ggcaacaact tcgcccttgc
    17581 gcagtctggc agcggtaaca gtgccaaccg caccgatttt aaaccacaaa aagccaacac
    17641 cagcgagatt ggcaccaaat ggcaggttct ggataaacgc ctgttgctca ccgccgcgct
    17701 attccgtact gatatcgaaa atgaagttga gcaaaatgat gacggaactt actcgcaata
    17761 cggtaagaaa cgtgtcgaag gctatgagat atccgtggcc gggaatatca cgcccgcgtg
    17821 gcaggtgatt ggcggctata cccagcaaaa agcaaccatc aaaaacggca aagatgttgc
    17881 ccaggatggt tcctcatcgc tgccgtatac cccggagcac gccttcacct tatggagcca
    17941 gtatcaggca acagatgata tctctgttgg cgcgggcgca cgctatatcg gcagtatgca
    18001 taaaggttca gacggcgcgg tgggaacgcc agcgtttacc gaaggttact gggtcgccga
    18061 tgccaaactg gggtatcggg ttaatcgcaa tctcgacttc cagctaaacg tttacaacct
    18121 gtttgatacc gattacgtcg cctcaatcaa caagagcggc taccgttatc acccgggcga
    18181 gccaagaacc ttcttgctca cagccaatat gcatttctga ttcagatgtg gggcgcaggc
    18241 cccacttttt ggagaacttg tatgatgtac cacattcccg gcgtgttatc gccacaggac
    18301 gtcgcccggt ttcgcgaaca actggaacaa gccgaatggg tggatggacg cgtcaccacc
    18361 ggcgcacaag gtgcgcaagt taagaacaat caacaggtcg acacccgcag cacgttatac
    18421 gccgccctgc aaaatgaggt gctgaacgcg gttaaccaac atgctttatt ctttgccgcg
    18481 gccttgccgc gtaccctttc cacgccgctg tttaatcgct atcagaacaa tgaaacctat
    18541 ggtttccatg tggatggcgc agtacgcagc catccacaaa acggctggat gcgtactgac
    18601 ctttctgcca cgctgttttt aagcgatcca caaagctacg acggcggcga actggtcgtt
    18661 aatgacacct tcggacaaca tcgggtaaaa ctcccggcag gcgatctcgt gttgtatccc
    18721 tccagcagcc tgcattgcgt gacacccgta acccgcggcg tacgagtggc atcatttatg
    18781 tggatccagt cgatgatccg tgatgataaa aagcgcgcca tgctgtttga actggacaac
    18841 aatattcagt cgctgaaaag ccgctacggc gaaagtgaag agatcctgtc gctgcttaat
    18901 ctttatcata atctgctgcg ggaatggtcg gagatctgat gcttgaactg cccaggttcg
    18961 tccctggatt agctacactt aactgtacaa gcattgatat ggggaggacg atatggcatc
    19021 cggttgggct aacgatgacg ccgtcaacga acagatcaac agtacaattg aagatgcgat
    19081 tgcccgcgct cggggtgaaa ttccgcgtgg cgaaagcctg gatgaatgtg aagagtgcgg
    19141 tgcccccatc ccgcaagccc gtcgggaagc cattcctggc gtgcgcttat gcattcattg
    19201 tcagcaggag aaaggtttac aaaaaccagc gtatacagga tatagtcgca gaggttcgaa
    19261 agacagccag ttacgttaac tacaactggc gagatgcata gcgagtataa tttgtatttt
    19321 gcgtggcatt ccgtgccttt aacgctgctg tgactgccac gcggcataaa acaagtccat
    19381 aagtacaaca aataaatggt ttatcagtat gttagatatc aatcaattta tttgaacaag
    19441 gcggtcaatt ctcttcgatt ttatctctcg taaaaaaacg tgatactcat cacatcgacg
    19501 aaacaacgtc acttatacaa aaatcacctg cgagagatta attatgaaaa ctatcaatac
    19561 tgttgttgct gctatggctc tttcaactct gtcatttggc gtgtttgccg cggaaccggt
    19621 aacggcatcc caggcacaga acatgaataa aatcggcgtg gtttctgccg atggcgcatc
    19681 caccctcgat gccctggaag cgaaactggc tgagaaagcc gcggcagccg gtgccagtgg
    19741 atacagcatc acttccgcca ccaacaacaa taaattaagc ggtactgcgg taatttataa
    19801 gtaattcgta ggacggataa ggcgtttacg ccgcatccgg caacagatgc ctgatgcgac
    19861 gctaccgcgt cttatcagta tctacataat ctctgcaatc tattgaattt gcatgttttt
    19921 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    19981 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    20041 tctctatagc caattgttcg cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    20101 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    20161 gcgcggttgc cagcaagagc gcacgcagtt ctggatgctg ttcgaattta gcgcgaagtg
    20221 cttttcgcat cacctgttct ttgaccgact cccagttttt acgcagaggc ttagaacgat
    20281 cgcgccccat gcgtgcggcg accatagggg aagaaacccg acggatctct tcgcggtatt
    20341 tttcatcaag gaatttttgt gcctgaaaat agtgttctga ggtgggccag gttttcccgt
    20401 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    20461 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    20521 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    20581 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    20641 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    20701 atccccgact ttattccccg tgcgccgcag cggcagatga ttgcggacgt cgccaaaacg
    20761 ctggccggag aagaagggcg acatctggcg attgaagccc ccaccggcgt tgggaaaacg
    20821 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    20881 agtaccgcca acgtggcatt gcaggatcag atttacagca aagatttacc gctgctgaaa
    20941 aagatcattc ccgatcttaa attcactgcc gcttttgggc gtgggcgcta cgtttgcccg
    21001 cgtaatctga cggcgctcgc cagcactgag cccacgcaac aggatctgct ggcgtttctt
    21061 gacgacgaac tgacgccaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    21121 gatctcgaca cttataaatg ggatggtctg cgcgatcata ctgatatcgc tatagatgac
    21181 gatctctggc gtcgtttaag taccgacaaa gccagctgcc tcaaccgcaa ctgttactac
    21241 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    21301 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactacc tgacccgaaa
    21361 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    21421 gagatgagtg ccgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    21481 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    21541 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    21601 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    21661 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    21721 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccacga tattgtgcgt
    21781 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    21841 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    21901 cgggaagagc gcgaagggca gctgcatctc tggtttcact gcgtgggaat acgtgtcagc
    21961 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    22021 ttgcgttcgc tgaacagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    22081 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    22141 attccccgga tgcgctttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    22201 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    22261 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg cctgatgctg
    22321 ctggtgcagg gcgatcagcc gcgttatcgc ttagttgaac tgcaccgcaa acgcgttgcc
    22381 aacggtgagc gtagtgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    22441 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccg
    22501 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    22561 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    22621 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    22681 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    22741 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    22801 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    22861 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgcccatat gctcaatggt
    22921 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggggaaggg
    22981 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    23041 cctgcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    23101 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    23161 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    23221 acgccaacgt tgcacggcgg acaggcgcag gcgaagctcg acgaacatca accggtgttt
    23281 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    23341 ggcgtgcgta acactgcaca taccctggcg aaactggcaa cgccatttgc cgaaggtgag
    23401 gcgctgcgtc tttccagcgt ttcgcatccg gaatacattg gacgcgtcgc gaagttcttt
    23461 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    23521 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    23581 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    23641 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    23701 atggcttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    23761 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    23821 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    23881 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    23941 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    24001 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    24061 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    24121 accatcatgc caaaaccgtt aaagagtcgg gggcggcggt cacgctcgat ggcgatcgcg
    24181 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcataagc
    24241 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    24301 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    24361 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    24421 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    24481 ttggtaaaac tcgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    24541 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggggcgc
    24601 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    24661 gggcgctttc tggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    24721 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    24781 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccgcatgat gatgataagc
    24841 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    24901 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    24961 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    25021 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    25081 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    25141 ggttcggatt ttgtagatac tgataagcgt agcgcatcag gcagttttgc gtttgttcat
    25201 cagcctgatg ccgggcatag cccggcataa aagattactg cgcagcggta ggtttacgcg
    25261 gacggcggcg acgttgttgt tcgcctgccg gtttggcatc gccgaggcgg cgagacggtt
    25321 tttctgcagg tttcgcgctt gcagattttg cgccaccttc cccacggcgt ggttgctgtt
    25381 gaccgcgacc accaccttgc ccacgaccgc cgtcgccacg ttgctggcga ccgttctgga
    25441 tcggttcggc tttgattgac gggtccggct catagcccgg aatcgcaatg cgcgggatct
    25501 cttttttcag cagtttttcg atatcacgca gcagtttgtg ttcatcaaca cacaccagcg
    25561 acaacgcttc accggtagca gccgcacgac cggtacgccc gatacggtgg acataatctt
    25621 ccggtacgtt tggcagttca tagttgacga cgtgcggcaa ctcttcgata tccagaccgc
    25681 gcgcagcgat atcggtcgcc accagtacgc gaatgtcacc cgatttaaaa tcagccagcg
    25741 cacgggtacg cgcaccttgc gatttattcc cgtggatcgc cgcactacgg atgccatctt
    25801 tattgagctg ctcagccaga tggttagcgc cgtgtttggt acgggtaaac accagcacct
    25861 gctgccagtt ccctttacca atcatgtgcg acagcaattc gcgtttgcgt ttcttatcaa
    25921 caaagtgaac gtgctgggtc acctgatcag acgcggtatt gcggcgtgcc acttcgattt
    25981 ccagcgggtt gtgcaacagt ttttccgcca gggctttaat atcgtcagag aaggtcgcag
    26041 agaacagcag gttctggcgc ttcgctggca gttttgttaa cacgcgacgg atatcgtgga
    26101 taaagcccat gtcgagcatg cggtcagctt catcgaggac gaggatttca acctgatcca
    26161 gcttcactgc attttgatgt tccaggtcca gcaaacgtcc cggtgttgcc accagaacat
    26221 caacgccgcc gcgcagcttc atcatttgcg ggttaatgct gacaccgcca aacaccacca
    26281 gcgaacgaat gttcaaatat ttgctgtaat cacggacgtt ttcgccaatc tgcgcagcca
    26341 gttcacgggt cggggtaaga atgagcgcac gtaccggacg acgccctttg gcgtgcggct
    26401 ggcgagtgat caggtgttgc aacagcggca gcgtaaagcc cgctgtttta ccggtaccgg
    26461 tctgggcgct agccatcagg tcgcggcctt ccagcaccgc agggatcgcc tgctgctgaa
    26521 taggggtggg ttcacggtaa ccctgctcgg caacggcgcg caggatatca gggcttaaac
    26581 ccaaagaatc gaaagacata actactccga accgccccga ccgttaccgg tgtagttttc
    26641 agggagatac tgaaaaggaa aatgacaaaa accacaatgt catgagggag cggagtgtag
    26701 cagtttttgt gacgcagcgc ataaattatc cctgtgactg gcgtagctaa aattcttagc
    26761 catactggac aactcccata tggagtcata atcttaatca atcatttgat taagaatgga
    26821 ctgtgcgatg aataatcctg ccatgacaat caagggtgaa caggcgaaaa aacagctgat
    26881 tgctgccgca ctggcgcagt ttggtgaata tggaatgaac gccaccaccc gcgagatagc
    26941 cgcccaggcc gggcagaata tcgctgccat cacctactac ttcggttcga aagaagattt
    27001 gtacctcgcc tgcgcccagt ggattgccga ttttattggc gagcagttcc gtccgcacgc
    27061 cgaagaggcc gaacgcttgt tcgcacaacc acagcctgat cgcgcagcca tccgtgaact
    27121 gatccttcgc gcctgcagga acatgattaa gctgctcacc caggatgaca ccgtcaacct
    27181 cagcaagttt atctcccgtg agcagctctc tcccacggca gcctatcacc tggtgcacga
    27241 acaggtgatt agcccgctac acagccacct gacacgcctg attgccgcct ggaccggctg
    27301 cgacgccaac gatacccgca tgatcctcca tactcatgcg ttgattggtg agattctggc
    27361 gttccgtctt ggcaaagaaa caattctgtt acgtaccggc tggaccgcgt tcgatgaaga
    27421 aaagaccgaa ctgatcaacc agacggtgac ctgtcatatc gacctgattt tgcaaggatt
    27481 atcgcaaagg agtttgtagt gatgaaaaaa cctgtcgtga tcggactggc ggtagtggta
    27541 cttgccgccg tggttgccgg aggctactgg tggtatcaaa gccgccagga taacggcctg
    27601 acgctgtatg gcaacgtgga tattcgtacg gtgaatctta gtttccgtgt tggggggcgc
    27661 gttgaatcgc tggcggtgga cgaaggtgat gctatcaaag cgggccaggt gctgggcgaa
    27721 ctggatcaca agccgtatga gattgccctg atgcaggcga aagcgggtgt ttcggtggca
    27781 caggcgcagt atgacctgat gcttgccggg tatcgcgatg aagaaatcgc tcaggccgcc
    27841 gcagcggtga aacaggcgca agccgcctat gactatgcgc agaacttcta taaccgccag
    27901 caagggttgt ggaaaagccg caccatttcg gcaaacgacc tggaaaatgc ccgctcctcg
    27961 cgcgaccagg cgcaggcaac gctgaaatca gcacaggata aattgcgtca gtaccgttcc
    28021 ggtaaccgtg aacaggacat cgcccaggcg aaagccagcc tcgaacaggc gcaggcgcaa
    28081 ctggcgcagg cggagttgaa tttgcaggac tcaacgttga tagccccgtc tgatggcacg
    28141 ctgttaacgc gcgcggtgga gccaggcacg gtcctcaatg aaggtggcac ggtgtttacc
    28201 gtttcactaa cgcgtccggt gtgggtgcgc gcttatgttg atgaacggaa tcttgaccag
    28261 gcccagccgg ggcgcaaagt gctgctttat accgatggtc gcccggacaa gccgtatcac
    28321 gggcagattg gtttcgtttc gccgactgct gaatttaccc cgaaaaccgt cgaaacaccg
    28381 gatctgcgta ccgacctcgt ctatcgcctg cgtattgtgg tgaccgacgc cgatgatgcg
    28441 ttacgccagg gaatgccggt gacggtgcaa ttcggtaacg aggcaggaca tgaatgatgc
    28501 cgttatcacg ctgaacggcc tggaaaaacg ctttccgggc atggacaagc ccgccgtcgc
    28561 gccgctcgat tgtaccattc acgccggtta tgtgacgggg ctggtggggc cggatggtgc
    28621 aggtaaaacc acgctaatgc ggatgctggc gggattactg aaacccgaca gcggcaatgc
    28681 cacggtgatt ggctttgatc caatcaaaaa cgacggcgcg ctgcacgccg tgctcggcta
    28741 tatgccgcag aaatttggtc tgtatgaaga tctcacggtg atggagaacc tcaatctgta
    28801 cgcggatttg cgcagcgtca ccggcgaggc acgtaagcaa acttttgctc gcctgctgga
    28861 gtttacgtct cttgggccgt ttaccggacg cctggcgggc aagctctccg gtgggatgaa
    28921 acaaaaactc ggtctggcct gtaccctggt gggcgaaccg aaagtgttgc tgctcgatga
    28981 acccggcgtc ggcgttgacc ctatctcacg gcgcgaactg tggcagatgg tgcatgagct
    29041 ggcaggcgaa gggatgttaa tcctctggag tacctcgtat ctcgacgaag ccgagcagtg
    29101 ccgtgacgtg ttactgatga acgaaggcga gttgctgtat cagggggaac caaaagccct
    29161 gacacaaacc atggccggac gcagttttct gatgaccagt ccacacgagg gcaaccgcaa
    29221 actattgcaa cgcgccttga aactgccaca ggtcagcgac ggcatgattc aggggaaatc
    29281 ggtacgtctg atcctcaaaa aagaggccac accagacgat attcgccatg ccgacgggat
    29341 gccggaaatc aacatcaacg aaactacgcc gcgttttgaa gatgcattta ttgatttgct
    29401 gggtggtgcc ggaacctcag aatcgccgct gggcgcaata ttacatacgg tagaaggcac
    29461 acccggcgag acggtgatcg aagcgaaaga actgaccaag aaatttggtg attttgccgc
    29521 caccgatcac gtcaactttg ccgttaaacg tggggagatt tttggtttgc tggggccaaa
    29581 cggcgcgggt aaatcgacca cctttaagat gatgtgcggt ttgctggtgc cgacttccgg
    29641 ccaggcgctg gtgctgggga tggatctgaa agagagttct ggtaaagcgc gccagcatct
    29701 cggctatatg gcgcaaaagt tttcgctcta cggtaacctg acggtcgaac agaatttacg
    29761 ctttttctct ggtgtgtatg gcttacgcgg tcgggcgcag aacgaaaaaa tctcccgtat
    29821 gagcgaggcg ttcggcctga aaagtatcgc ctcccacgcc accgatgaac tgccattagg
    29881 ttttaaacag cggctggcac tggcctgctc gctgatgcat gaaccggaca ttctgtttct
    29941 cgacgaaccg acgtccggcg ttgatccgat tactcgccgc gaattctggc tacatatcaa
    30001 cagcatggta gagaaaggcg tcacggtgat ggtcaccacc cactttatgg atgaagcaga
    30061 atattgcgac cgcatcggcc tggtgtaccg tgggaaatta attgccagcg gcacgccgga
    30121 tgatttgaaa gcgcagtcgg ccaacgacga gcaacccgat cccaccatgg agcaagcctt
    30181 tattcagttg atccacgact gggataagga gcatagcaat gagtaacccg atcctgtcct
    30241 ggcgtcgcgt acgggcgctg tgcgttaaag agacgcggca gatcgttcgc gatccgagta
    30301 gctggctgat tgcggtagtg atcccgctgc tactgctgtt tatttttggt tacggcatta
    30361 acctcgactc cagcaagctg cgggtcggga ttttactgga acagcgtagc gaagcggcgc
    30421 tggatttcac ccacaccatg accggttcgc cctacatcga cgccaccatc agcgataacc
    30481 gtcaggaact gatcgccaaa atgcaggcgg ggaaaattcg cggtctggtg gttattccgg
    30541 tggattttgc ggaacagatg gagcgcgcca acgccaccgc accgattcag gtgatcaccg
    30601 acggcagtga gccgaatacc gctaactttg tacaggggta tgtcgaaggg atctggcaga
    30661 tctggcaaat gcagcgagcg gaggacaacg ggcagacttt tgaaccgctt attgatgtac
    30721 aaacccgcta ctggtttaac ccggcggcga ttagccagca cttcattatc cccggtgcgg
    30781 tgaccattat catgacggtc atcggcgcga ttctcacctc gctggtggtg gcgcgagaat
    30841 gggaacgcgg caccatggag gctctgctct ctacggagat tacccgcacg gaactgctgc
    30901 tgtgtaagct gatcccttat tactttctcg ggatgctggc gatgttgctg tgtatgctgg
    30961 tgtcagtgtt tattctcggc gtgccgtatc gcgggtcgct gctgattctg ttttttatct
    31021 ccagcctgtt tttactcagt accctgggga tggggctgct gatttccacg attacccgca
    31081 accagttcaa tgccgctcag gtcgccctga acgccgcttt tctgccgtcg attatgcttt
    31141 ccggctttat ttttcagatc gacagtatgc ccgcggtgat ccgcgcggtg acgtacatta
    31201 ttcccgctcg ttatttcgtc agcaccctgc aaagcctgtt cctcgccggg aatattccag
    31261 tggtgctggt ggtaaacgtg ctgtttttga tcgcttcggc ggtgatgttt atcggcctga
    31321 cgtggctgaa aaccaaacgt cggctggatt agggagaaga gcatgtttca tcgcttatgg
    31381 acgttaatcc gcaaagagtt gcagtcgctg ctgcgcgaac cgcaaacccg cgcgattctg
    31441 attttacctg tgcttattca ggtgattctg ttcccgttcg ccgccacgct ggaagtgact
    31501 aacgccacca tcgccatcta cgatgaagat aacggcgagc attcggtgga gctgacccaa
    31561 cgttttgccc gcgccagcgc ctttactcat gtgctgctgc tgaaaagccc acaggagatc
    31621 cgcccaacca tcgacacaca aaaggcgtta ctactggtgc gtttcccggc tgacttctcg
    31681 cgcaaactgg ataccttcca gaccgcgccg ttgcagttga tcctcgacgg gcgtaactcc
    31741 aacagtgcgc aaattgccgc caactacctg caacagatcg tcaaaaatta tcagcaggag
    31801 ctgctggaag gaaaaccgaa acctaacaac agcgagctgg tggtacgcaa ctggtataac
    31861 ccgaatcttg actacaaatg gtttgtggtg ccgtcgctga tcgccatgat caccactatc
    31921 ggcgtaatga tcgtcacttc actttccgtc gcccgcgaac gtgaacaagg tacgctcgat
    31981 cagctactgg tttcgccgct caccacctgg cagatcttca tcggcaaagc cgtaccggcg
    32041 ttaattgtcg ccaccttcca ggccaccatt gtgctggcga ttggtatctg ggcgtatcaa
    32101 atccccttcg ccggatcgct ggcgctgttc tactttacga tggtgattta cggtttatcg
    32161 ctggtgggat tcggtctgtt gatttcatca ctctgctcga cacaacagca ggcgtttatc
    32221 ggcgtgtttg tttttatgat gcccgccatt ctcctttccg gttacgtttc tccggtggaa
    32281 aacatgccag tatggctgca aaacctgacg tggattaacc ctattcgcca ctttacggac
    32341 attaccaagc agatttattt gaaggatgcg agtctggata ttgtgtggaa tagtttgtgg
    32401 ccgctactgg tgataacggc cacgacaggg tcagcggcgt acgcgatgtt tagacgtaag
    32461 gtgatgtaac ttcttatctt tcgccagcaa agacactacc gccgggccgg caaggattgc
    32521 cagccctgca attgccagca aaaagttgtt ttccagtaca cgggacaaca gccacagcac
    32581 aatcagcgct gcgccaaaat accaggtcgt ggtcagctct tccagcacgt ctgccacctc
    32641 acgccagcgt tgctcggggt ggcgctgaaa caccaacatc atcactaccg tgacacaata
    32701 cagtgcgcat aatcccacgc cgacgtgaac cagagtctta ctcatccagc ccaccaccag
    32761 cagcgccact accagtaaat gcaacataat ctgccagcaa cttagaccag ttgcgacacg
    32821 aacacgttgt tgccacttca tggcttctct cctgaagaat ttatctctac ttactcagag
    32881 taccgaactt tacggaaaat tccgtaagac cgcacctttt tgtgacgccg actacactat
    32941 ttcaatgaga taagaggcaa agacaggaaa attatgcccg atcaaacaca acaattttcg
    33001 ttcaaggtgc tcaccatcaa cattcacaaa ggttttaccg cgtttaaccg acgcttcatt
    33061 ttgccggaac ttcgcgacgc ggtgcgtacc gtcagcgccg atattgtttg cctgcaggaa
    33121 gtgatgggcg cgcacgaagt tcatccgctg catgtggaaa actggcccga tacctcgcac
    33181 tacgagtttc tcgccgacac gatgtggagc gattttgcct acggtcgcaa tgccgtatac
    33241 ccgaaagggc atcacggcaa cgccgtactg tcgcgttatc ccattgaaca ttatgagaat
    33301 cgcgatgttt cggtcgatgg tgcggaaaag cgcggcgtgc tctactgccg cattgtgcca
    33361 ccgatgaccg gaaaagcgat tcatgtgatg tgcgtacatc tgggcctgcg tgaggcgcac
    33421 cgtcaggcgc agcttgcgat gctcgccgaa tgggtgaatg agctaccgga cggcgaaccg
    33481 gtattggtgg cgggtgattt caatgactgg cggcaaaaag ctaatcatcc gttaaaagtg
    33541 caggccggac tggatgagat ttttacccgc gcccacggac gcccggcgcg cacgtttccg
    33601 gtgcaatttc ctctactacg actggacagg atctacgtca aaaatgccag cgccagcgcg
    33661 ccaaccgcgt tgccgctgcg gacatggcga cacctttctg atcatgcccc tttaagtgcg
    33721 gagattcatt tatgaaatgt agctggcgcg aaggcaataa gatccagttg ctggaaaacg
    33781 gcgagcaata ttatcccgcg gtgtttaagg cgattggcga ggcacaagaa cgcatcattc
    33841 ttgaaacgtt tatctggttt gaggatgacg tcggcaaaca gctgcatgcg gcactactgg
    33901 cagcagcgca acgcggggtt aaagcggaag tcttgctgga tggctacggt tcgccggatc
    33961 tcagcgatga gtttgtcaat gaactgacgg cagctggcgt ggtatttcgc tactacgatc
    34021 cccgccctcg cctttttggt atgcgcacca atgtgtttcg ccggatgcat cgcaaaattg
    34081 tggtgatcga cgcgcgtatc gcctttattg gcgggctgaa ttactccgcc gagcatatgt
    34141 ccagctacgg tccagaggct aaacaggatt acgcggtacg ccttgaaggg ccgattgtcg
    34201 aagatattct ccagtttgag ctggaaaacc tgcctggaca gagcgcggca cgacgctggt
    34261 ggcgacgtca tcacaaagcg gaagagaacc gccagccggg agaagcgcag gtattgctgg
    34321 tctggcgcga taacgaagaa catcgcgatg atattgaacg tcactatctg aaaatgctca
    34381 ctcaggcgcg gcgagaagtc attatcgcca acgcctactt cttccccggc tatcgatttt
    34441 tacacgcctt gcgtaaagcg gcacggcgcg gggtgcggat caaactgatc attcagggcg
    34501 aaccggatat gccgattgtc agagtcggtg cgcgcttgct gtataactat ctggttaaag
    34561 gcggcgttca ggtatttgag taccgccgcc gcccgctcca cggcaaagtg gcattgatgg
    34621 acgatcactg ggcgacagta gggtccagta atctcgatcc gctcagtttg tcactgaatc
    34681 tcgaagcaaa tgtcatcatc cacgatcgtc attttaacca gacgctgcgc gataatctga
    34741 acggcattat tgccgcagat tgtcagcagg tcgatgagac catgctgccg aaacgcacct
    34801 ggtggaacct gaccaaaagc gtgctggcgt tccacttttt acgccacttc ccggcgctgg
    34861 ttggctggct tccggcacac acaccacgtc tggcgcaggt tgatccgccc gcacaaccga
    34921 caatggaaac gcaggatcgg gtagaaactg aaaacacggg ggtaaaaccc tgatgagtaa
    34981 atcacacccg cgctggcgct tagcaaagaa gatcctcacc tggctgtttt ttatcgcggt
    35041 gattgtgttg ctggtggtct acgccaaaaa agtggactgg gaagaggtct ggaaggtcat
    35101 ccgcgactac aatcgcgttg cgctgcttag tgcggtcggg ctggtggtcg tcagctatct
    35161 gatttacggc tgctatgacc tgctcgcccg cttttactgc ggtcacaaac tggcgaagcg
    35221 ccaggtgatg ctggtgtcgt ttatctgcta cgccttcaac ctgacgctca gtacctgggt
    35281 cggcggcatt ggtatgcgct atcgtttgta ctcccgacta gggttaccgg gcagcactat
    35341 tacgcggatt ttctcgctca gtattaccac caactggctg ggctatattt tactggcagg
    35401 gattatcttt accgcaggcg tggtggagtt gccggatcac tggtatgtcg atcaaactac
    35461 gctgcgtatt ctcggcattg gcttactgat gattatcgcg gtttatttgt ggttttgcgc
    35521 tttcgcgaag caccgccata tgaccatcaa agggcaaaag ctggtgctgc cttcatggaa
    35581 attcgccctc gctcagatgc tgatttccag cgttaactgg atggtaatgg gggcgattat
    35641 ctggctgtta cttggtcaaa gcgtgaacta tttctttgta ctgggcgtgt tactggttag
    35701 tagtattgct ggcgtcatcg tgcatattcc ggtggggatc ggtgtgctgg aagcggtgtt
    35761 tatcgcacta ctggctgggg agcatacctc gaaagggagc attatcgccg ccctgctcgc
    35821 ttatcgcgtc ctgtattact ttatcccgct gctgctggcg ctgatctgtt acctgctact
    35881 ggaaagccag gcgaagaagc tacgggcgaa aaatgaagcg gcgatgtgaa gtttagttaa
    35941 gttctttagt atctgcattt acgactaatg aaaaaaacgc gtatgccttt gccagacaag
    36001 cgttatagct atcgcggcaa gtatcccaaa gaagccgata aaatagtaat agagtgttaa
    36061 tgcacctgta acagcgcaac gaaccggact ccacaactct tcactatggc atttccccgc
    36121 caattcaagg agcttatttc gtccatgcga gataatgcct gaccataaca caatcattaa
    36181 gcaacttatt acccaaacgg gtcgttcact cattaatgcg gcattcatga tgatcaccag
    36241 ggccaaccct gtcaacgtca tcataatgat aaaacggtga gaaccaggat caacattaaa
    36301 taataaaccg gccagcatac tgattaaaaa catcgcgccc gcagtgctga acacgacgat
    36361 aatggcaggt acaggagcta aacttgcaac gacaatcgcc accaaagaga gtaaaaacag
    36421 atatattacg cctgcgatga cggcatttat cttgttgata tccttactta agccatacat
    36481 acaaactaat aataacccta tggcgacata cattgttggt gaatcatatt tggttgccgt
    36541 aatttcgaga tacttagctc cccaggccag tcctgcggtt gccaccaaac ctaaagcggt
    36601 aatgagccat ataccgttta atatcctggt ctcgtgctga aaatctaatt tattgctgtt
    36661 ttgtgagtat gattccattc ttttagttgt ccttagaaaa aatattaata ttcaccatca
    36721 ataaatttaa aaccacgcat acaaaatact tttgtattta tggatacgga aaacgcttcc
    36781 gctcgccat
//
LOCUS       DAZGJW010000032        36460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZGJW010000032 DAZGJW010000000
VERSION     DAZGJW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.032"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1169
                     /locus_tag="ACHKIK_001658"
     CDS             <1..1169
                     /locus_tag="ACHKIK_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO9082850.1"
                     /translation="ARKNGTYTDLPQNVDTAEDSVQPEPVKVTADEVNKIMQAANISQ
                     PDADKLLAASRGEFVEGISDPNDPKWVKGIETCDSVNQNQQESEQNDQKTEQNSPNAQ
                     QNEPETKQEPEKACTACGQTGGGSCPDCGAVISDATYQEIFDEENQPEVQENDPEEME
                     GTAHQHKENTGGNQHHASDSETGEASDPLIKANGHHNITSTSRAGIHLMIDLETMGKN
                     PDAPIISIGAIFFDPQTGEMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAILT
                     DEIPLDDALLQLREFIDENSGEFFVQVWGNGATFDNVILRRSYERQGIPCPWRYTNDR
                     DVRTMVALGLVMDFDARTTIPFEGERHNALNDARYQAKYVSAIWQKLLPSQADF"
     gene            1237..1479
                     /locus_tag="ACHKIK_001659"
     CDS             1237..1479
                     /locus_tag="ACHKIK_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO9082851.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            1457..2476
                     /locus_tag="ACHKIK_001660"
     CDS             1457..2476
                     /locus_tag="ACHKIK_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9082852.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            2884..3543
                     /gene="yccA"
                     /locus_tag="ACHKIK_001661"
     CDS             2884..3543
                     /gene="yccA"
                     /locus_tag="ACHKIK_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO9082853.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            3634..3963
                     /gene="tusE"
                     /locus_tag="ACHKIK_001662"
     CDS             3634..3963
                     /gene="tusE"
                     /locus_tag="ACHKIK_001662"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO9082854.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(3960..4238)
                     /gene="yccX"
                     /locus_tag="ACHKIK_001663"
     CDS             complement(3960..4238)
                     /gene="yccX"
                     /locus_tag="ACHKIK_001663"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO9082855.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMLWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            4333..5523
                     /gene="rlmI"
                     /locus_tag="ACHKIK_001664"
     CDS             4333..5523
                     /gene="rlmI"
                     /locus_tag="ACHKIK_001664"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO9082856.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEIFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            5581..5898
                     /gene="hspQ"
                     /locus_tag="ACHKIK_001665"
     CDS             5581..5898
                     /gene="hspQ"
                     /locus_tag="ACHKIK_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO9082857.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(5943..6356)
                     /gene="yccU"
                     /locus_tag="ACHKIK_001666"
     CDS             complement(5943..6356)
                     /gene="yccU"
                     /locus_tag="ACHKIK_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO9082858.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            6529..7191
                     /gene="yccT"
                     /locus_tag="ACHKIK_001667"
     CDS             6529..7191
                     /gene="yccT"
                     /locus_tag="ACHKIK_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO9082859.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            7287..7745
                     /gene="mgsA"
                     /locus_tag="ACHKIK_001668"
     CDS             7287..7745
                     /gene="mgsA"
                     /locus_tag="ACHKIK_001668"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO9082860.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(7777..9831)
                     /gene="helD"
                     /locus_tag="ACHKIK_001669"
     CDS             complement(7777..9831)
                     /gene="helD"
                     /locus_tag="ACHKIK_001669"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO9082861.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            9954..10400
                     /gene="yccF"
                     /locus_tag="ACHKIK_001670"
     CDS             9954..10400
                     /gene="yccF"
                     /locus_tag="ACHKIK_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO9082862.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            10410..12572
                     /gene="yccS"
                     /locus_tag="ACHKIK_001671"
     CDS             10410..12572
                     /gene="yccS"
                     /locus_tag="ACHKIK_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO9082863.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(12535..13164)
                     /gene="sxy"
                     /locus_tag="ACHKIK_001672"
     CDS             complement(12535..13164)
                     /gene="sxy"
                     /locus_tag="ACHKIK_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO9082864.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            13383..13892
                     /gene="sulA"
                     /locus_tag="ACHKIK_001673"
     CDS             13383..13892
                     /gene="sulA"
                     /locus_tag="ACHKIK_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO9082865.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            14249..15289
                     /gene="ompA"
                     /locus_tag="ACHKIK_001674"
     CDS             14249..15289
                     /gene="ompA"
                     /locus_tag="ACHKIK_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO9082866.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(15364..15816)
                     /gene="matP"
                     /locus_tag="ACHKIK_001675"
     CDS             complement(15364..15816)
                     /gene="matP"
                     /locus_tag="ACHKIK_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO9082867.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            16002..17762
                     /gene="ycbZ"
                     /locus_tag="ACHKIK_001676"
     CDS             16002..17762
                     /gene="ycbZ"
                     /locus_tag="ACHKIK_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9082868.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLSDFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            17831..18349
                     /gene="fabA"
                     /locus_tag="ACHKIK_001677"
     CDS             17831..18349
                     /gene="fabA"
                     /locus_tag="ACHKIK_001677"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO9082869.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18419..18586)
                     /gene="rmf"
                     /locus_tag="ACHKIK_001678"
     CDS             complement(18419..18586)
                     /gene="rmf"
                     /locus_tag="ACHKIK_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO9082870.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18842..19405)
                     /gene="pqiC"
                     /locus_tag="ACHKIK_001679"
     CDS             complement(18842..19405)
                     /gene="pqiC"
                     /locus_tag="ACHKIK_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO9082871.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASAWSQEAASIAQEIKRLP"
     gene            complement(19402..21042)
                     /gene="pqiB"
                     /locus_tag="ACHKIK_001680"
     CDS             complement(19402..21042)
                     /gene="pqiB"
                     /locus_tag="ACHKIK_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO9082872.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(21047..22300)
                     /gene="pqiA"
                     /locus_tag="ACHKIK_001681"
     CDS             complement(21047..22300)
                     /gene="pqiA"
                     /locus_tag="ACHKIK_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO9082873.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(22430..24337)
                     /gene="uup"
                     /locus_tag="ACHKIK_001682"
     CDS             complement(22430..24337)
                     /gene="uup"
                     /locus_tag="ACHKIK_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9082874.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(24349..26457)
                     /gene="rlmKL"
                     /locus_tag="ACHKIK_001683"
     CDS             complement(24349..26457)
                     /gene="rlmKL"
                     /locus_tag="ACHKIK_001683"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO9082875.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLGGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            26701..27810
                     /gene="ycbX"
                     /locus_tag="ACHKIK_001684"
     CDS             26701..27810
                     /gene="ycbX"
                     /locus_tag="ACHKIK_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO9082876.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PGAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            complement(27807..28349)
                     /gene="zapC"
                     /locus_tag="ACHKIK_001685"
     CDS             complement(27807..28349)
                     /gene="zapC"
                     /locus_tag="ACHKIK_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO9082877.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(28523..29533)
                     /gene="pyrD"
                     /locus_tag="ACHKIK_001686"
     CDS             complement(28523..29533)
                     /gene="pyrD"
                     /locus_tag="ACHKIK_001686"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO9082878.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(29644..30381)
                     /gene="ycbF"
                     /locus_tag="ACHKIK_001687"
     CDS             complement(29644..30381)
                     /gene="ycbF"
                     /locus_tag="ACHKIK_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO9082879.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(30347..30862)
                     /gene="ycbV"
                     /locus_tag="ACHKIK_001688"
     CDS             complement(30347..30862)
                     /gene="ycbV"
                     /locus_tag="ACHKIK_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9082880.1"
                     /translation="MLKRIIWILCLLGLTGSSELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGMDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(30870..31412)
                     /gene="ycbU"
                     /locus_tag="ACHKIK_001689"
     CDS             complement(30870..31412)
                     /gene="ycbU"
                     /locus_tag="ACHKIK_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9082881.1"
                     /translation="MKKKTIYQYVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(31424..32494)
                     /gene="elfG"
                     /locus_tag="ACHKIK_001690"
     CDS             complement(31424..32494)
                     /gene="elfG"
                     /locus_tag="ACHKIK_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9082882.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNEGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(32485..35085)
                     /gene="elfC"
                     /locus_tag="ACHKIK_001691"
     CDS             complement(32485..35085)
                     /gene="elfC"
                     /locus_tag="ACHKIK_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO9082883.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            complement(35110..35811)
                     /gene="elfD"
                     /locus_tag="ACHKIK_001692"
     CDS             complement(35110..35811)
                     /gene="elfD"
                     /locus_tag="ACHKIK_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9082884.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            complement(35894..36436)
                     /locus_tag="ACHKIK_001693"
     CDS             complement(35894..36436)
                     /locus_tag="ACHKIK_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9082885.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAAKKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
BASE COUNT         9017 a         9425 c         8709 g         9309 t
ORIGIN      
        1 ttgcccgcaa aaacggaaca tacacggact taccgcaaaa tgtggatacc gctgaagatt
       61 ccgtacaacc ggagccggta aaagttaccg ctgacgaagt aaacaaaatt atgcaggcag
      121 ccaatatcag ccagcctgac gccgataaat tgcttgctgc atcacgtggt gaatttgttg
      181 aagggattag cgatccgaat gatccgaaat gggtgaaggg gattgaaacc tgcgattctg
      241 tgaaccagaa ccagcaagaa tcggaacaga acgaccagaa aacggaacaa aacagcccaa
      301 atgcgcaaca aaacgagcca gaaacgaaac aggagccgga aaaagcctgc accgcctgcg
      361 gtcagaccgg cggcggtagc tgccctgatt gtggtgcggt gataagtgac gcaacatacc
      421 aggaaatatt cgatgaagag aatcagcctg aagttcagga aaatgatccg gaggaaatgg
      481 aaggcactgc gcatcagcac aaggagaaca ctggcggcaa tcagcatcat gccagcgata
      541 gtgaaactgg cgaggcgtca gatcccttaa ttaaggcgaa cggtcatcat aatatcacat
      601 ccaccagcag agcggggatt catctgatga tcgaccttga aaccatggga aaaaatcctg
      661 atgccccgat tatctcaata ggtgcaatat ttttcgatcc gcaaaccgga gagatggggc
      721 cagaattcag caaaactatc gatctggaaa ctgctggcgg agtcattgat cgggacacca
      781 ttaagtggtg gctgaaacag tcacgcgaag cgcaatccgc cattcttacc gatgaaatcc
      841 cgttagatga tgcactgctg caattacggg aatttatcga cgaaaactcc ggtgaatttt
      901 ttgttcaggt ctggggtaac ggtgcaactt tcgacaacgt gattttacgc cgttcatatg
      961 aacggcaggg gatcccctgc ccgtggcgtt acaccaatga tcgcgatgta agaacgatgg
     1021 ttgctctggg actggtgatg gatttcgacg caagaacgac tattccattc gaaggtgaac
     1081 gccataacgc tctgaacgat gcacgttacc aggcgaaata cgtttcagcc atctggcaaa
     1141 aactgctccc gagtcaggct gatttttgat gttcaaccca tatcgccgcc caccagctat
     1201 agtggcggcg gtcatgctgt aaaggcacgt gaccacatgt acgaattaac tctatctcca
     1261 gcagagatta aagagatcac gaaatacgag cgatacacaa aacagcaaca ccagttaaga
     1321 ctgcacggca tcccatttgt aatcggccct aaaaacgaac ccatagttct ccgcagggat
     1381 attccacacg gtctgacaac gatgccaaaa acatctgaac tggtttctgc tgaacccgat
     1441 tttgaggcgc tgaacaatgg gaagaccaag aaaaaataaa aaagataatg tactgccacc
     1501 gcgggttaga tcgaatggtt acagttacgt gtggaaaccc gaaggaagta caagaagtat
     1561 agggctagga agagtgcgga aaaccagcgt agctaaagtc tggcaaaatt atgaactgga
     1621 aaaagcaaaa ctccacaaca taatgaccgt agctaaatta tggcacatgt ttatggactc
     1681 ccctgcattt acagaactgg ccccccgaac ccaaaaagat tatcgacaac atcagaaggc
     1741 gttgctgatg gtattcggaa aagtgcttgc tgataatgtc aaaactgagc aggtaagaat
     1801 tttcatggat aaacgagggc ttgagagcaa gacccaggca aatcatgaac tggcaagcct
     1861 gagtcgagta tacgggtggg gatatgagcg tgggtatgtg aagaataacc catgcaaagg
     1921 agtcagaaaa ttctctctta aagcccgcac tgtttacatc accgatgaac agtatgcggc
     1981 gatatatgcg gaagcaattc cacagttacg cattgcaatg gagatttcct atctctgtgc
     2041 ggcaagactc ggtgatgtgc ttgagttgaa atggcaggat attatggata aagggatcta
     2101 cattgagcaa aacaaaaccg gcaccaaaca aatcaaggaa tggtcaccgc gattacgtac
     2161 agcgatccag ttagcccgaa atgtatcttc ctgtacatgc gaatatgtga tcaatacaac
     2221 caaaggcggg aaagtcatag ctaaaacgct gaataactgg tggaatcagg ctaaacgcgc
     2281 agccgagcaa aaagttggcg ttccgttcgg gtgcaatttt cacgacataa aagccaaggg
     2341 gatctcagat tacgaaggca gcagtcgcga caaacaaatt ttcagcgggc ataaaacaga
     2401 aaatcaggtg ttgatttacg atcgtaaaac aaaaatcaca ccaacactgg atttgccgct
     2461 cgtggttagc aagtagacaa tccagaaaaa tttctggcaa aaagacttgg tatactagct
     2521 atgagcaact tcctaatacc ttgatttttc ttggtgttaa atttgattaa cttttcagca
     2581 atttcacgta ttgcactgat ttttatttgt ttttcttcgg tcttgaaaac cggcgacccg
     2641 aaagggttcc agagttcgaa tctctgcgct tccgccaaat aagataaggg gttagctaaa
     2701 tgctaacccc tttttctttt gcctgtcgaa attctcaggg cgttatattt gcttaatgac
     2761 ctgataatcc gctgttaaac ctgttccaga tcaaatgcgt aaagatgggt aaaacttctg
     2821 ggtgccctta cgcattatca ttatgctgct taattaatta catctgtcat agagagtgac
     2881 tcaatggatc gtattgttag ttcttcacat gaccgtacat cactgcttag cacccataag
     2941 gtgctgcgta atacctattt tctgctgagc ctgacgctgg ccttttcggc aattaccgca
     3001 actgccagta cggtgctgat gctgccatct ccgggtctga ttctgacgct ggtgggtatg
     3061 tatggtttga tgttcctgac ctataaaacg gcgaataagc cgaccgggat tatctccgca
     3121 ttcgccttta ccggttttct gggttatatc ctcgggccta ttctgaacac ctatctgtct
     3181 gccggaatgg gtgacgtaat cgctatggca ctgggcggaa cggcgttagt gttcttctgc
     3241 tgctctgcat atgtgctgac cacccgcaaa gatatgtcgt tcctcggcgg tatgctgatg
     3301 gcgggtattg tggtggtgct gattggtatg gttgctaata tcttcctgca gctgcctgct
     3361 ctgcatctgg cgatcagcgc ggtcttcatt ttgatctcct ctggcgctat tttgtttgaa
     3421 accagcaaca tcgttcatgg cggtgaaacg aactatattc gtgccacggt tagcctgtat
     3481 gtttcgctgt acaacatctt cgtcagcctg ctgagcattc tgggcttcgc cagccgcgat
     3541 taatctcacc cgctaacact ttcatagcct cgctttatgc ggggctttgt tttttgttac
     3601 actgccgcca gtctgattaa ggtagtaata attatgctga tcttcgaagg taaagagata
     3661 gaaacggata ccgaaggcta tctcaaagaa agcagccagt ggagtgagcc gctggcagtg
     3721 gtgattgcag agaacgaagg gatttcgctg tcaccggaac actgggaagt ggtgcgtttt
     3781 gtgcgtgatt tctatctgga attcaatact tctccggcga ttcgtatgct ggtaaaagcg
     3841 atggcgaata aatttggcga agaaaaaggt aacagccgct atttataccg actgtttccg
     3901 aaaggtccgg ctaagcaagc caccaaaatt gctggcctgc caaaaccggt aaaatgtatt
     3961 taacggatac gaaaatcggt taattccccc gagggatgat gcggctcgct gagcacccgt
     4021 tctacacgcg cagaacgcgg gccgccactc tttaaccaca gcattaattt ttccacctgc
     4081 ccttcttcac cgcaggcaac cacttccacg ctgccatcgt caagattttt ggcgtacccg
     4141 gttaaaccca gtcttttcgc ttcgtactgt gtggtgtagc gaaatcctac gccctgaact
     4201 cgcccgtaaa cccaggcaat tatgcagact ttcgacattg ctgctctcct tatccttaat
     4261 ggacgcgttg caaatccatg gaaaactccg gacaatgacg cccatttctt tgcatcgaca
     4321 gaaaagtaaa ttatgagtgt acgtttagtg ttagccaaag ggcgcgaaaa atcattactt
     4381 cgtcgccatc cgtgggtctt ttccggggcc gttgcccgca tggaaggtaa agccagcctc
     4441 ggtgaaacca tcgatattgt tgatcatcag ggaaaatggt tagcacgcgg cgcttattca
     4501 ccagcttcgc aaatccgggc gcgcgtctgg acgtttgacc cgtctgagtc tatcgacatt
     4561 gctttttttt cccgccgttt gcaacaagca caaaaatggc gtgactggct ggcgcaaaaa
     4621 gatggcctcg acagctatcg tttaatcgcc ggagaatctg atggcctgcc gggtattact
     4681 atcgatcgtt tcggtaattt tctggtgctg caactgctga gtgctggggc agaatatcag
     4741 cgcgcggcat tagttagtgc cctgcaaacg ctgtacccgg aatgtgcgat ttacgatcgc
     4801 agcgatgttg cggtacgtaa aaaagaaggg atggagctga cccagggcct cgtcaccggc
     4861 gagttgccgc ctgccctgct gccgattgaa gaacacggca tgaagctgct ggtggatatt
     4921 cagcacggac acaaaacggg ctactacctg gaccagcgtg atagccgcct ggctacccgc
     4981 cgctacgttg aaaataaacg tgtgctgaac tgtttctcct ataccggtgg tttcgccgta
     5041 tcggcactga tgggcggttg cagccaggtt gtcagcgttg atacctccca ggaagcgctg
     5101 gatattgcac ggcagaacgt tgagctgaac aaactggatc tgagcaaggc tgagtttgtc
     5161 cgtgatgatg tctttaaatt gctgcgtact tatcgcgatc gcggtgaaat atttgacgtt
     5221 atcgtgatgg acccgccgaa gtttgttgag aataaaagcc agttgatggg cgcgtgtcgt
     5281 ggctataaag atatcaacat gctggcgatt cagttgctga atgaaggcgg tattctcctg
     5341 actttctcct gttccggtct gatgaccagc gatttatttc agaaaatcat cgcggatgcc
     5401 gcaattgatg ccggccgtga tgtacaattt atagagcagt tccgtcaggc agccgatcat
     5461 ccggtgatcg ctacctatcc ggaagggcta tatctgaaag ggtttgcctg tcgcgtcatg
     5521 taacttgaaa tgtggaattt tacccttata tcaaaggtaa agatttcccg ggaggtgact
     5581 atgattgcca gcaaattcgg tatcggccag caggtccgcc attccctgtt aggttatctc
     5641 ggagtggtcg tggatatcga cccggtttat tcgctctctg aaccgtcgcc tgatgagctg
     5701 gcggtgaatg acgagcttcg cgccgctccg tggtatcacg tggtgatgga ggacgataac
     5761 ggcctaccgg ttcataccta cctggctgaa gcccagctaa gtagcgaatt gcaggatgaa
     5821 catcctgaac agccatccat ggatgagctg gcgcagacca tccgcaaaca actccaggcc
     5881 ccgcgtctgc gtaactgatc cctgaacgaa tccccggaag gtttttgcct tccggggatt
     5941 ttttatttgg ccaggcccag gcgagggatc tcaatagccg ggcagcgatc catcaccaca
     6001 ttcaacccgg catcccgtgc cagtactgcc gcttgctcat taatcacccc cagttgcatc
     6061 cagagggttt tcgcgccaat ggcaatagct tcctgtgcca cgccccacgc cgcttctgaa
     6121 ttacggaaaa catcgaccat atcgaccttt tccggaacgt ctgccagcgt gccatacccc
     6181 tgctggccca gcagcgtttt gccggcaact tttggcgaaa ccggaatgac gtgatacccc
     6241 tggtcgagta gatatttcat cacgcgatag ctgggacgat cgggtttgtc gcttgccccc
     6301 accagcgcaa tggtgtgagt ggacgttaaa atgcctgcaa tatcggtttc tttcattatt
     6361 tcctccagac tgtttaacaa agtgtacgac aaagccgaca tgccaaccat gcagctaata
     6421 cgaatgaatg agagcaaaag cgggaaatat gtctatatgt tagtaagtat gattaaagaa
     6481 aaattttgac acattattat tcgggcggtc ttttgacagg agctacctat gaaaaccggc
     6541 atcgtgacca ccttgattgc attatgtttg ccggtatccg tttttgccac cacgctgcgg
     6601 ctttcaaccg atgtcgatct tctggtactc gacggcaaga aggtttccag ctctctgctg
     6661 cgcggtgcgg acagcattga actggataat ggaccgcatc agttagtgtt tcgcgttgag
     6721 aagacgattc atctttccaa tagtgaagaa cggctgtata tctccccacc gctggtggtc
     6781 agttttaata cccagctcat caaccaggtc aattttcgcc tgcctcgcct ggagaatgag
     6841 cgggaagcta accattttga tgccgcgccg cgccttgaat tgttggatgg cgatgcgacg
     6901 ccgattccgg taaagctgga tattctcgcc attacctcaa cggcaaaaac gattgattat
     6961 gaggtcgagg ttgaacgcta taacaaatcc gcgaaacgcg cttcactacc gcaatttgcc
     7021 acgatgatgg cagatgacag tacgctgctt tcgggtgttt ccgagctgga tgctattcca
     7081 ccgcagtctc aggtgctcac agaacaacgg ctgaagtatt ggtttaaact ggctgaccca
     7141 caaacgcgaa atactttcct gcagtgggcg gaaaaacaac catcttcctg agatttttgt
     7201 gcctgtgcgc aggctttttc ggtctttatc ttgcagcgat aagtgcttac agtaatctgt
     7261 aggaaagtta actacggatg tacattatgg aactgacgac tcgcacttta cctgcgcgga
     7321 aacatattgc gctggtggca cacgatcact gcaaacaaat gctgatgagc tgggtggaac
     7381 ggcatcaacc gttactggaa caacacgtac tgtatgcaac aggcactacc ggtaacttga
     7441 tttcccgtgc aaccggcatg aacgtcaacg cgatgttgag tggcccaatg gggggtgacc
     7501 agcaggttgg cgcattgatc tcagaaggga aaattgatgt attgattttc ttctgggatc
     7561 cactaaatgc cgtgccgcac gatcctgacg tgaaagcctt gctgcgtctg gcgacggtat
     7621 ggaacattcc ggtcgccacc aacgtggcaa cggcagactt cattatccag tcgccgcatt
     7681 tcaacgacgc ggtcgatatt ctgatccccg attatcagcg ttatctcgcg gaccgtctga
     7741 agtaatattg cacaggtggc aaacgccacc tgtttcttac ggttttctcg ccaccggcac
     7801 atccagattt ttcagtattt ccacaaaggg agagggattc tctttgttaa acagtgccca
     7861 tacccgatgg cgtgcccggg tcagcgccac gtacattaac cgccgttctt cagcgtccgg
     7921 gaaatcctca accggtggca gtagcgcctc ttccataatc gactcccgcg ccgcagccgg
     7981 aaaaccatca ctcccctcct gcaagccaac gatgatgacg taatccgcct gttgcccttt
     8041 gctggcatga atggtcataa agtcgatttg caacttcggc cagcgtgttg ccgctttttc
     8101 caggctggca ggcctcatgt gatggtaacg cgccaggata agaatgcgct cttccggttt
     8161 ggcataacca gagagcttat ccagcaaagc gtcaagttga ctctcatcca ataacgtgac
     8221 ggctttttcg tctccattgg ttaagctgtt tagcggcttt ttcaactggc ctgggttctg
     8281 ctgaataaac cggtttgcca cctcaccgat acgactgtta aaacggtaag tcgtgtctaa
     8341 atcacagcga tcgccttcac caaagttttc atggaaagcg gtggtgagcg acatttgcgc
     8401 gccgctgaat cggtaaatcg cctgccagtc atcaccaacg gcgaacaacg tcgtctgact
     8461 gttttgcttg cgtaatgccg ctaacaacgc tgcccgctgc ggcgagatat cctgaaattc
     8521 atcaaccaga atatgcttcc acgggctgat aaagcgacct ttctccagaa tcacaatcgc
     8581 ctgatgaata aggcccgaaa aatcgacagc gttttctgcc ttcagcgcac ctttccaggc
     8641 ttttaataac ggggccatca acttgatacg tttactgaac agatcgcgaa tctcttcggg
     8701 tgcactggca atcatttctg cctgtgcacc accgtgcatc cgcatcagac ttacccagcg
     8761 atcgaggcga gaggcaaggc gacgctgtaa tttttcatca tcccagaagt taccttctgg
     8821 cactgaccac tgcatttctt ccgtcagcca ttgccgccag cccttcgcct gcgctttctt
     8881 ttcgctgcat tgcttgcgcc actcagcaat aaagagttca tgacgggcag cggtatcatt
     8941 ttccagtttg ctgactatcg gaactttttt gctgccctgc tgaataatat gcagcgccag
     9001 cgcatgaaac gtgcgtgcgg taatgtcttc ggtatgtagc cgttcgcgaa tccgctcgtc
     9061 catctcttca gcggctttgc gaccaaacgc cagcaataaa atttgctcag gggacgcttc
     9121 accacgcgcc agcaaccagc ctgcacgggc caccagcacc gacgtttttc cgcttcctgc
     9181 acctgccagc actaacagag aatgctcgcc attaacgact gcccgggcct gcgccggatt
     9241 cagcggtgaa gactcgacct ggcggaaaaa atccgcatac tcggtaagca tggcttcggt
     9301 atacgcctgg ttatgctgca accgagcgct ttcaatatct ttcaaccagg cctgacattt
     9361 acgccacgcc tcacggcagt tatcgaattc ttccagtcgg ttaaccggca acggcaacgc
     9421 cgacaaagcc tggcggattt gttgctgcac gccagaggtt tgctcacgcg tcagccattt
     9481 attttcccca gtgcgcgtgg caatcaaatc cagttgctgg cgtaaaacac cagacgcaat
     9541 ttcgctcatc tcgccactcc accgccgcca gtgagcatca agatgatggt aaaaacgctg
     9601 cgtctcgccc cattcggtgc cgtgcagacg caccactttt tcgtccggca gtacaaattc
     9661 cagctcgccc cataccagac cgcgcttaca gtgaatcgcc agtaattgat tgaaaggaat
     9721 aaggtattcg tggcgatcgc cggagacttt aatcccggca ttgaggatca ccgcccgatc
     9781 gtaagggtgc tgtgccagac gttttccaag cgttgtcgct ttcagttcca tgactctgta
     9841 gatccacacg aaattatttc ttatcagtgt aaccgtcaga gaaaacgtgc tccagcccaa
     9901 aaaaacgtta caattgccgc ccattactga aaaccacagt aaagcgaggt tttatgcgta
     9961 ccgttttgaa cattctgaac tttgtgcttg gcggatttgc caccactctg ggctggctgt
    10021 tggcgactct ggtcagtatt gtgctgattt ttaccttacc gctgacacga tcctgctggg
    10081 agatcactaa actgtctctg gtgccttatg gcaatgaagc tattcatgtc gatgaactga
    10141 acccggctgg caaaaatgta ctgctgaata ctggcggtac ggtattgaat attttctggc
    10201 tgattttctt tggctggtgg ttatgcctga tgcacattgc aacgggcatc gcacaatgta
    10261 tttcaatcat tggcattcct gtcggcattg cgaactttaa aattgccgct attgcactat
    10321 ggccggttgg tcgtcgcgtg gtatcggtag aaacagcgca agctgcgcgt gaagccaatg
    10381 cacgtcgtcg ttttgaataa tccactaata tggcctttat gctaagtcct ttgctcaaac
    10441 gctatacctg gaacagcgcc tggctgtatt acgcgcgtat ttttattgcg ctttgtggaa
    10501 ccacagcgtt tccgtggtgg ctgggtgatg taaaactgac gattccgcta acgctgggga
    10561 tggtggcagc ggcgctgacc gatctcgatg accgactggc gggacgtttg cgtaacctca
    10621 tcattacgct gttctgcttt tttatcgcct cggcctcagt agaattgctg tttccctggc
    10681 cctggctatt tgcgattggc ttaacgctct ctaccagcgg cttcattttg ctcggcggtc
    10741 tgggtcaacg ctatgcaaca attgccttcg gtgcattgct gatcgccatt tacactatgt
    10801 tgggaacatc actgtatgag cactggtatc agcagccgat gtatctgctg gccggtgccg
    10861 tctggtacaa cgtcctgaca cttattggtc atctgctgtt cccggtccgc ccgctgcagg
    10921 acaacctggc gcgttgctat gaacaactgg cgcgttatct tgagctcaag tcgcgcatgt
    10981 ttgatcctga tattgaagat gaaagccagg caccgctgta cgatttggct ctcgccaacg
    11041 gtctgctgat ggcgacattg aatcagacga aactctcgct gctgacccgc ttacgtggcg
    11101 atcgtggtca acggggaacg cgtcgcacgc tgcattatta ctttgtcgca caggatattc
    11161 acgagcgtgc cagctcttct catattcagt atcaaacatt gcgtgaacat tttcgccaca
    11221 gcgacgtgct gttccgtttt cagcggctga tgtcgatgca gggccaggcg tgccagcaac
    11281 tgtcacgctg tattttgttg cgtcagcctt atcaacatga tccgcatttt gagcgcgctt
    11341 ttacgcatat tgatgctgcg ctggagcgga tgcgcgataa cggcgcaccc gccgatttac
    11401 tcaaaacact gggatttttg ctgaacaatt tacgcgccat tgatgcccaa ctggcaacaa
    11461 ttgaatcaga acaggcccag gcactacccc ataataatga cgaaaatgag ctcgctgatg
    11521 acagcccgca cgggttgagt gatatctggc tgcgtcttag ccgtcacttc acgccggaat
    11581 ccgccctctt ccgtcatgcg gtaagaatgt cgctggtgtt gtgcttcggc tacgccatca
    11641 ttcaaataac cggaatgcat cacgggtatt ggatcttgct gacaagtttg tttgtctgcc
    11701 agccaaacta taacgccacg cgccaccgcc tgaagttaag gattattggt acgctggtag
    11761 gtatcgccat tggcattcct gtgctgtggt ttgtgccatc actggaaggg cagctggtgc
    11821 tgctggttat taccggcgtg ctcttttttg ccttccgtaa cgtgcaatac gctcatgcaa
    11881 cgatgttcat cacacttttg gtgctactgt gttttaactt actgggtgaa ggttttgaag
    11941 tagcgttacc tcgcgtaatc gatacgctga ttggttgtgc cattgcgtgg gcggcagtga
    12001 gctacatctg gcctgactgg cagtttcgca atctgccgcg catgctcgaa cgcgccacag
    12061 aggccaactg tcggtatctc gatgccatac tggagcaata ccatcagggg cgtgataacc
    12121 gtctggcgta tcgtattgcc cgccgcgatg cacacaaccg tgatgctgag ctggcgtcgg
    12181 tggtatcaaa tatgtccagc gagccgaacg ttaccccgca aattcgcgaa gccgcgtttc
    12241 ggttgctgtg ccttaaccat acgtttacca gctatatctc agccctcggt gctcaccggg
    12301 agcagttaac taatcctgaa attctggcgt ttcttgatga cgcagtttgc tatgttgatg
    12361 acgcgttaca tcatcaacct gctgatgaag aacgcgtcaa tgaggcatta gctagcctga
    12421 aacagcggat gcagcaactt gaaccacggg cagacagcaa agaacctctg gtcgtacaac
    12481 aagttggatt attgattgca ttactgcctg agattggtcg tctgcaacgc cagattactc
    12541 aagttccgca ggaaactcct gtttcggcgt aagagagtca gcccattctg caagctcctg
    12601 gcggcgtgcc accgggagcg cagcttcatg aatgccgata atggcacctt caagcataaa
    12661 cagaatcttt tctgtcacca gactgttttg ctgccgcagt cgcaaccaac acatttttgc
    12721 cccaagtata cgtaacgccc gtacatcctt aatccccacc tccccgagaa tcgcttccag
    12781 atgaaaagac atattgggca aatctttcag tctttcccgg gtattgcgcg tgcttttctc
    12841 tttcagcgct gcatcgagag aatatttcga cagacgcacc agcttcagtt gatttcgcca
    12901 tagactttca tcaacccgat agtaattgag ggtaacggat cggccacact ttttatatgt
    12961 cagccagaca ggcggatgtt ttacacagta ctgtgcactt tgctcacaag cccgaagata
    13021 caactcacca tcagaaacca tcgcaaacac cgtgtcgtca acggtcaggc tgtaactgcc
    13081 aaacaatgat cggtattcaa ttgtgcccaa cgttgccagg tattcttgtg atttatagat
    13141 ccgcttatag gagaggcttt tcataaaatt ccttttaaaa tcataacata aaagaatgat
    13201 tcacattaac ggatccgtta actacgaaaa taggcaactt attcttaagg ggcaagatta
    13261 atttatgttt tcccgtcacc aacgacaaaa tttgcgaggc tctttccgaa aatagggttg
    13321 atctttgttg tcactggatg tactgtacat ccatacagta actcacaggg gctggattga
    13381 ttatgtacac ttcaggctat gcacatcgtt cttcgtcgtt ctcatccgca gcaagtaaaa
    13441 ttgcgcgtgt ctctacggaa aacactacag ccgggcttat cagtgaagtt gtctatcgcg
    13501 aagatcagcc catgatgacg caacttctac tgttgccatt gttacagcaa ctcggtcagc
    13561 aatcgcgctg gcaactctgg ttaacaccgc aacaaaaact gagtcgggaa tgggttcagg
    13621 catctgggct acccttaacg aaagtaatgc agattagcca gctctcccct tgccacactg
    13681 tggagtcaat ggttcgcgct ttacgcacgg gcaattacag tgtggtgatc ggttggttgg
    13741 cagatgattt gactgaagaa gagcatgctg aacttgttga tgcggcaaat gaaggtaacg
    13801 ctatggggtt tattatgcgt ccggtaagcg catcctctca cgccacgaga caactttccg
    13861 ggctaaaaat tcactctaat ttgtatcatt aagtaaattt aggattaatc ctggaacttt
    13921 ttttgtcgcc cagccaatgc tttcagtcgt gactaatttt ccttgcggag gcttgtctga
    13981 agcggtttcc gcgattttct tctgtaaatt gtcgctgaca aaaaagatta aacatacctt
    14041 atacaagact tttttttcat atgcctgacg gagttcacac ttgtaagttt tcaactacgt
    14101 tgtagacttt acatcgccag gggtgctcgg cataagccga agatatcggt agagttaata
    14161 ttgagcagat cccccggtga aggatttaac cgtgttatct cgttggagat attcatggcg
    14221 tattttggat gataacgagg cgcaaaaaat gaaaaagaca gctatcgcga ttgcagtggc
    14281 actggctggt ttcgctaccg tagcgcaggc cgctccgaaa gataacacct ggtacactgg
    14341 tgctaaactg ggctggtccc agtaccatga cactggtttc atcaacaaca atggcccgac
    14401 ccatgaaaac caactgggcg ctggtgcttt tggtggttac caggttaacc cgtatgttgg
    14461 ctttgaaatg ggttacgact ggttaggtcg tatgccgtac aaaggcagcg ttgaaaacgg
    14521 tgcatacaaa gctcagggcg ttcaactgac cgctaaactg ggttacccaa tcactgacga
    14581 cctggacatc tacactcgtc tgggtggtat ggtatggcgt gcagacacta aatccaacgt
    14641 ttatggtaaa aaccacgaca ccggcgtttc tccggtcttc gctggcggtg ttgagtacgc
    14701 gatcactcct gaaatcgcta cccgtctgga ataccagtgg accaacaaca tcggtgacgc
    14761 acacaccatc ggcactcgtc cggacaacgg catgctgagc ctgggtgttt cctaccgttt
    14821 cggtcagggc gaagcagctc cagtagttgc tccggctcca gctccggcac cggaagtaca
    14881 gaccaagcac ttcactctga agtctgacgt tctgttcaac ttcaacaaag caaccctgaa
    14941 accggaaggt caggctgctc tggatcagct gtacagccag ctgagcaacc tggatccgaa
    15001 agacggttcc gtagttgttc tgggttacac cgaccgcatc ggttctgacg cttacaacca
    15061 gggtctgtcc gagcgccgtg ctcagtctgt tgttgattac ctgatctcca aaggtatccc
    15121 ggcagacaag atctccgcac gtggtatggg cgaatccaac ccggttactg gcaacacctg
    15181 tgacaacgtg aaacagcgtg ctgcactgat cgactgcctg gctccggatc gtcgcgtaga
    15241 gatcgaagtt aaaggtatca aagacgttgt aactcagccg caagcttaag ttctcgtctg
    15301 gtagaaaaac cccgctgctg cggggttttt ttgcctttag taaattgaac tgactttcgt
    15361 cagttattcc ttacccagca atgcctgcag atcctgcttc agagaagaca ttttattcgc
    15421 gtatttctct ttgttttccg catcttcaat cagctgaaca atcgtttcag aaagcgtttt
    15481 accgcgacgc tgcgcaagac cagccagacg ttgccagaca ataaattcca gatcgatcga
    15541 ttttttgcgc gtatgctggt gttccgcatt aaaatggcgc tttcgtcttg cccgaatggt
    15601 ttgcttcatg cgattgacca gttccggatt catatgcttg tcaatccagc catttaccag
    15661 cacgggttca ttttcgagcg agagcaacac atcgacggct tcctgggcgg cactggcttc
    15721 tatgtaacgg gtgattaact ccccttcgcg atgcttcttc accagatact tccatttcca
    15781 accgctttca agattttcaa gttgttgata tttcatagcg atctcaatgt taccgtgtaa
    15841 ctctttacag aatatcagct ttttaggcca gtgaagaaaa gaatctccat cctgtgagct
    15901 ttaacgccca tgccaggagt attgtcggtg ctttctacgt gtgctgtagt gccggttacg
    15961 gtataatcgc ggctttgaca acagactaaa aaacatcaac tttgaccatt acgaaacttg
    16021 catggcgtga cctggttcct gataccgata gctatcagga aatatttgct cagccacatt
    16081 tgattgacga aaacgatcct ttattcagtg atactcaacc gcgactgcaa ttcgcactgg
    16141 agcagttgct gcatacgcga gcatcctcct cttttatgct ggcgaaggcc ccggaagagt
    16201 ctgagtatct gaatcttatt gccaatgccg cgcgtacgct acaaagcgat gcaggccaac
    16261 tggtgggcgg tcactatgag gtttccggcc actccatccg cttacgtcac gcagtgagtg
    16321 cagatgataa ttttgcgact ttaacgcaag ttgtcgctgc cgactgggta gaagcggagc
    16381 aactctttgg ctgcctgcgc cagtttaatg gcgacattac cctgcagcct ggtctggtgc
    16441 atcaggcaaa tggcggtatt ctcatcatct ctttgcgtac actgctggcg caacctctgc
    16501 tgtggatgcg gctgaaaaat atcgttaacc gcgagcgttt tgactgggtt gcgtttgatg
    16561 agtcgcgccc tctccccgtc tctgtgcctt cgatgccatt gaagctgaaa gtcattctgg
    16621 taggcgaacg cgaatcattg tctgatttcc aggagatgga gccagagctt tcagagcagg
    16681 ctatttatag cgaatttgaa gatactctgc agattgtcga tgcagagtca gtaacccagt
    16741 ggtgtcgctg ggtgacattt accgccagac ataatcactt acctgcaccg ggagcggatg
    16801 cctggccggt acttatccgc gaagcagcac gctacaccgg tgaacaagaa acacttccgc
    16861 ttagcccgca gtggatcctc cgccagtgta aagaggtcgc ctccctgtgt gatggcgaca
    16921 ccttctccgg cgagcagcta aacttaatgc tgcagcagcg tgaatggcgc gaaggtttcc
    16981 tcgctgaacg tatgcaggat gagatccttc aggagcaaat cctgattgaa accgaaggcg
    17041 aacgcatcgg gcaaattaac gccctttcgg tcattgaatt tccgggtcat ccacgcgctt
    17101 ttggcgaacc ttctcgcatt agctgcgttg tgcatattgg cgatggtgaa ttcaccgaca
    17161 tcgaacgcaa agcggagctt ggcggcaata tccatgcgaa agggatgatg atcatgcaag
    17221 cgttcctgat gtcggaacta cagcttgagc aacagatccc cttctcagca tcgctgacat
    17281 ttgagcagtc atacagtgaa gttgatggag atagtgcctc gatggctgaa ctctgcgccc
    17341 tgataagcgc cctcgccgat gtgccggtga atcagagtat cgctatcaca ggttcagtcg
    17401 atcagttcgg tcgcgcccag ccggtcggtg gtttaaatga gaaaatcgaa ggcttctttg
    17461 ctatttgcca gcaacgtgag ttaaccggga aacaaggtgt cattatcccc acagctaacg
    17521 ttcgccattt aagtcttcac agtgaactgg tgaaagcggt agaagaaggc aaattcacca
    17581 tctgggcagt agacgatgtg actgacgcac tgccgttatt attaaatctg gtgtgggatg
    17641 gcgaaggcca aacgacgctg atgcaaacca tccaggaacg tatcgcgcaa gcatcgcaac
    17701 aggaaggacg tcaccgtttt ccatggccat tacgttggct gaactggttt attccgaact
    17761 gatcggactt gttcagcgta cacgtgttag ctatcctgcg tgcttcaata aaataaggct
    17821 tacagagaac atggtagata aacgcgaatc ctatacaaaa gaagaccttc ttgcctctgg
    17881 tcgcggtgaa ctgtttggcg ctaaaggccc gcaattgcca gcaccgaaca tgctgatgat
    17941 ggaccgtgtg gtcaaaatga ccgaaacggg tggtaacttc gacaaagggt atgttgaagc
    18001 agaactggat atcaatccgg atctgtggtt cttcggatgc cactttattg gcgatccggt
    18061 tatgccggga tgcctgggcc tggacgcaat gtggcagctg gtagggttct acctcggctg
    18121 gctgggcggc gaaggtaaag gccgcgcgct gggcgttggc gaagtgaaat tcactggtca
    18181 ggtactgccg acagcgaaaa aagtgaccta ccgtattcac tttaaacgca ttgttaaccg
    18241 tcgtctgatt atgggcctgg cggatggcga agtgctggtt gatggtcgtc tgatctatac
    18301 cgccagcgac ctgaaagtcg gtctgttcca ggatacgtct gccttctgat tataatcaga
    18361 cagagtatca aaaggcgaaa cctccgcaat gcggaggttt ctttttaaag agacagaatc
    18421 aggccattac taccctgtcc gccatggctt ctcgccagcc tcccagccat tgtgaccttt
    18481 gattcagcgt ctgataggga cacatttctt ttgagcgtcc ggcgatgccg gcctgataac
    18541 cacgttgatg tgcccgttcc aggcgatctc gtttttgtct cttcatgcct cgtttccctc
    18601 atactggttt ctggtggaaa agaaaacagt gattactaaa tgtgcaatca cagttaagca
    18661 ataccgcgaa tcataactgc cgtcaatgcg caaaattcac gccactgtca tatttgtgag
    18721 ctacacgggt atgccggttg tacaaaaaat gtgaagagga acaacttttt ccgcacgtta
    18781 gagacaaaaa aaccgcctca ggcaacatac cggaggcggt tatatttaca aatctttata
    18841 attaaggtag acgctttatc tcttgcgcaa tagaagcggc ttcctgactc caggcactgg
    18901 ccagtacttt aaccatctca tcgtaaccat cctgagtttg caccccttcc agacggaacg
    18961 gacgtttgat cagttgtccc tggtggttca acagccactc accactgaca atgaccttgc
    19021 catcatagcg accgttaaac tccgtgacgg taacattgag cgtgtcctgg gcgcttccca
    19081 gaggctggga ggcaaccacc cagccgggca gttgcgtact caggttggca accagggtgt
    19141 tgcgcaactg ttgatccaac gggctggccc acaagttgtt attggcaatc acatacttca
    19201 catcactggt ttgataaacc acaccattcc ccgccagata gtcaggaaca gtgacctgct
    19261 ctacccataa cagacgattg ccctggctgg cggtactttg tgtaccgctc tgcaccacag
    19321 gtaactggta atagttttta ttaatttcgc cggagctgca tccggccagc cacagtgctg
    19381 caatcgtcac tagccacttt ttcattgttt cgccctcttc ggctctggat cttttttgtc
    19441 cttcgcttca aataccagcg cgttactctt ctcattgagc gttttcagca ccggttgcag
    19501 ttctcgcaac acctgatcaa ggcgctgcat atccgccacc atcttgttgt aggctgcgga
    19561 gccaggctgg aagccctgca tgctgcgatt caattcacgc aacgttgact gcatatccgt
    19621 cggcaactgc tgcatcgact ggctagcgag gatcttgttc atgctatcca gcgtcgtttg
    19681 caggtttttc attgtgcgct gactttcaga aagcgtactg gttgcctgtt caatcatcgg
    19741 attcaatggc agtttgttga tcttatccaa cgcttccatc agtcgttgct ggatttgcgc
    19801 caggccgccg ctaacggtcg ggataatctg ataaccatta aattcacgaa taccggttat
    19861 tgcaggcgta tttggataga aatcgagatc aacatacagt gcaccagtga ccaggtttcc
    19921 ggttttcagc gatccgcgta aaccacgttt caacaattcg ccaaggtgct caacaacatc
    19981 cgcattttcg ccaagctgca ttttcagccg ctctggctcg atacgaatca gtaccggaat
    20041 acggtaatca tcgttaaatg tctgacgcat attcggcgca aagaatggca ctttgcttac
    20101 ggtacccagg cgaatacccc ggaactctac cggagctccc ggttgcagac cgcgtaccga
    20161 atctttaaag aacatcagat aatcaatgtg atcggtgtac aacgaatcct gaatgctctt
    20221 ctgatcatca tacaaaacga aagctgtttt cggtgccact ggctgcccta aatccagacc
    20281 ttccggcaca tcaaagctga caccgccact cagcagcgtt gtcaatgagc ccatctccac
    20341 acgcatccct gctgacgtca gatcaaccgc aatgccacta tctttccaga agcgaacatt
    20401 gttggtcacc agtcggtcat aaggtgcatt gatgaacagt tgatagctga tattgcgttt
    20461 ttgtgtatcg aaggtgctgg tttcaaccga acctacccga tagccacgga acagcaccgg
    20521 atctcctggc gagagctgcc cggctttttt gctatcgaga atcacacgga tacctttcgc
    20581 atcaggcggg gccaacggtg gcgagtccag caaatcgtat ttatccattt tgctgccttt
    20641 cgcgcctggc tgcagttcga tataaacccc agacagcagg gttcccagac cgctaatccc
    20701 ttctcgacca atctgcggtt tcaccaccca aaagacggtg tctttatgca gcaatttttc
    20761 cataccggaa ttcagccgcg ctttgatttc aacgtgcgtc aaatcatcag ccagtgtggc
    20821 gctttcaacc acgccgacgt caacgctacg gcttttaatg gtggttttgc caccttcaat
    20881 tccttccgca ttcgcggtga tcagggtcac ttccggtccc tgatggctgt aatgataaaa
    20941 aagaacccag gccccaatga gcgccgtgac gataggaaat atccacacgg gagaccagtt
    21001 cttcactttc tggattttgg cttccccatt attagattcc atactgtcag gactcctcat
    21061 gctctgattc aggttgacga tcccacgaca aacgcgggtc aaacgtcata gcagaaaaca
    21121 ttgtcattat gacgactaaa gcaaacatta atgcacccat tgccggataa atactcatta
    21181 aacctcccat acgcaccagc gccgagagca ccgcgataac gaaaacgtca atcatcgacc
    21241 agcggcctac aaactcaaca acttcataaa tcaaatgcat tctttcactg tcgcgcttgc
    21301 catgcccttt ggcatcccaa cacagccacg cgatggcgat catctttaac gttggcacca
    21361 taatactggc cagaaagatc accgcagcga cgggataaga gccttcgctc cataacagaa
    21421 tgaccccagc gagaatcgtc gacggcatct tcgagcctaa taaatccgtc accatgatgg
    21481 gcaaaatatt agccggaaga tacagcatga tggacgttac aagcagcgcg agtgtccact
    21541 gcaggctgtt tctacgccga acgtaccctt tggtactaca acgcgggcac acgggttcat
    21601 cagcaggaag gattgccgta caacaggagc acgaacgcag cccctgacgt atccccgtga
    21661 cgcctggttt tagcggctgg cgcagttctg gcatcggggc gatgtcgtcc cataaccagc
    21721 gacgatcaac gcactgaaaa gcgcgcagtt gcaggacaca aaataaacac caggggagaa
    21781 agctgctgcc taccccaatg ctgccgtaag ccatcagttt aacgaaactg accagcacac
    21841 cagcgaggaa aatctccgcc attccccagg ttttgagttg aaaaagcacc cgtgccagtt
    21901 gctcttttaa acggaccggt aattccgcgc gattcaccag taacagaatg gttatcagac
    21961 aaaacgcggg aaccagttgc acaaacaaca ggaaaaaggt gccgaggctg gcgtagtcct
    22021 cagaaaaaag cacgccggga atttccagta atgtaatttc actggtaact cctgcaacgt
    22081 tcatattcac aaaaggaaac aagttggaca gcaacagcat gaacagtgca gccaacgcat
    22141 aggcggtggg acgctgccga ggggcatccc acgccacggt taacgttgtg ccacaccggg
    22201 gacatgccgc tttctgacca tgctcaaggc gcggtaacgc caccagcatg tcacactgcg
    22261 agcacaggat gtgcttcgcg gcatgatgat gttcgcacat tggtactcct taatgatgcg
    22321 ctattttaca gatgactaac aaagtgttgg ttttcatgcc tgataaaccc agttcatcag
    22381 gcaattttac cgttgtcagc tgtctctgtt taaatcgact attttgcgat cagccaccat
    22441 tttttaacgc ttcaagatac tcccagcgtt caaaggcttg ctccagctcc tgctctgcag
    22501 cagccatatc ggcgagcact ttttgtgtct gctcatgtgg ctgactgaag aaggaagcat
    22561 ccgccacctg cgtctgtagg gcttccagct tcgcctccag atcttcgagc aattgcggta
    22621 gctgctccag ttcgcgctgc aatttatagc ttagtttgct actgctgcgt tttacagttt
    22681 ctgcttttgc cgcagcggct tcttcggttt ttttcaccgc aggctgtttg agcgccacat
    22741 actgctcttg ctgaccacgg gcatcatgat aaccgccgac ataacgacca attttaccgc
    22801 cgccttcgaa gatccaacat tctgtaacgg tgttatcgac aaactgacga tcgtggctaa
    22861 ccagcaatac cgtgccctga tagctgtcga tcagttcttc cagcagttcc agcgtttcga
    22921 catcaagatc gttggtcggt tcgtcaagaa tcaataagtt gcttggtttg aggaacaaac
    22981 gcgccagcag caagcggttc cgctcaccgc cagaaagcgc acgtaccggc gtcatcgccc
    23041 gtttcggatg gaacagaaag tcctgcaaat agcccaatac gtggcgtggc ttgccgttaa
    23101 ccatcacctc ttgcttacct tcggcaaggt tatccatcac cgttttatcg ggatccagtt
    23161 ccgcgcggtg ctgatcgaaa taagccactt ccagtttggt gccaacgtga atacgcccgc
    23221 tgtccgcttg aagctgaccg agcatcagtt tcagcagcgt ggttttgccg cacccattcg
    23281 gaccaatcag ggcaattttg tcgccacgta gaacctgggc cgaaaaatct ttcaccagtt
    23341 gcttaccgtc aacctggtag caaacgtctt ccatttcgaa aacgatctta ccggagcggc
    23401 tggcctcttc cacctgcatc tttgcggtac ccatcacttc gcgacgttca ccacgctcgc
    23461 gacgcattgc tttcagggcg cgtacgcggc cttcattacg ggtacggcgt gctttgatcc
    23521 cctggcgaat ccacacttct tcctgcgcca gtttgcgatc gaactcggca ttttgtagtt
    23581 cttccacgcg cagggcttct tctttttcct gcaggtactg gtcgtaattc cccggatagg
    23641 tcaccagctt gccgcgatcg agatcaacaa tgcgcgtcgc catattgcgg ataaacgaac
    23701 ggtcgtggga gatgaaaata atcgtcccat tgaaggtttt aaggaaccct tccagccagt
    23761 cgatggtttc gatatccaga tggttagtcg gttcgtccag cagcaatacg cgcggattgc
    23821 tcaccagcgc gcgtcctaat gccgctttac gcaaccagcc gccggaaagc gacgacagcg
    23881 caacattagg atctaacccc agttgcgcca gcacttcgtt gatgcggttt tccagctgcc
    23941 acaagttgtg gtgatccagc tgttcctgaa ccttcgccag ttcgttgaga tttttctcgc
    24001 tcgggtcgtt catcaccagg cgcgaaatat cgtgatagcg tttcagatat tccgcttgtt
    24061 cttcaatgcc ttcggcaacg aaatcataaa cgctaccctc aacgttacgc ggcggatcct
    24121 gttgcagacg agctacaatc aaatcttgct cgtaaataat gcgaccgtca tccagccctt
    24181 gttcacggtt gaggattttc attaacgtcg atttgcctgc gccgttgcgg cctaccagac
    24241 aaacacgttc attatcttcg atatgcagtt ctgcgttatc gagaagcggc gcgtcgctga
    24301 acgacagcca ggcgccatgc atactgatta atgacattac tattcctttc aggctgcggt
    24361 aatcagccag cagttgtgga tctggcggtt acgggcgaaa tcctgggaga gcgttttttg
    24421 cgtaatttct tgtgctttca gtcccagttt tgccaggccg ccgagatcca tacggaagcc
    24481 acgtttgttg ttcgagaaca tgatcgtccc acctgcacgc agcagacgtt tcaaatcttt
    24541 catcagcgcc agatgatcgc gctgaacatc aaacgcatct tccattcgtt ttgagttaga
    24601 gaaggttggc ggatcgataa agatcagatc gaactgttca tttgcctcac gcagccacgc
    24661 caggcaatcg gcctgaatca ggcgatgcgc acgcccggtc aggccattca gacgcaggtt
    24721 gcgttctgcc cactccagat aagtacgcga catatccacg gtggtggtgc tgcgtgcacc
    24781 gcctaatccc gcatgcacgg tggcgctgcc ggtataagag aacaggttga ggaaatcttt
    24841 gcctttgctc atctggccga gcatacgacg ggcgatacgg tgatcgagaa acagaccggt
    24901 atcgaggtaa tccgtcaggt tcaccaacaa gtgagcgtta tattcggtaa cttcaagaaa
    24961 ctcgcccttc tcgcccagtt tctggtactg atttttgccc ttctggcgtt cacgggtttt
    25021 cagcaccagt ttgtttggcg caatccccag taccgaaatg gttgcagcga taatatcgaa
    25081 cagacgctga cgcgctttgt gagcatcaat agtttttggc ggcgcatact cctgcaccac
    25141 cacccagtcg gcataacggt caacggcaac gttatattcc ggtagatcgg cgtcatacag
    25201 gcggtaacat tcaattcctt cctggcgagc ccacttctcg aattttttga ggttcttacg
    25261 caggcggttg gcgtagtctt ccgctaccat cgccggtttg ctgtctggag tgctttcggc
    25321 aacgtggtaa tttttctgta cgcagtccag cgggccgttt ttcgccttgt actgtttgtc
    25381 tgcacgcagc tgcaagcagc ttagcagatc cggcgaggca ctaaacaaag agagattcca
    25441 gccaccaaac tggtttttca tgatccgacc cagcaggcta tgcagcgcaa tcagcgccgg
    25501 ttcgctgtcc agacgttccc cgtatggcgg gttgctcaac actgtgccgt acggcccttt
    25561 cggcagcgga ttggtcagtt gcgcgacatc tttcacctca aaggtgatca gttcaccaat
    25621 ccccgcaaga cgggcgttag tgcgtgcacg ttgaatcacc cgtgcgtcgc tgtccgaacc
    25681 gtaaaaatga gagctatact cagccaggcc tttacgggcg cgagtttgcg cttccgcttt
    25741 cacttcctgc cagatagctt catcatgctg cgcccagccg ctaaagcccc aacgcccacg
    25801 gtgcaagcct ggtgcgcgat cggtcgccag catcgccgct tcaatcagca acgtaccgga
    25861 accacacatc ggatcgagca gcggtgttcc tggctgccag ccggatcgca tcacaatagc
    25921 ggctgccagg gtttctttga tcggcgcaat accagcacga tcgcgatagc cacgcagatg
    25981 taaaccatca ccactcagat caagagcgat actggcggtt tctttatgca gccagacgtt
    26041 aacgcggata tccggcgcat cgcgatcaac atttggacgc ggcagatttt tccgcgtgaa
    26101 agcatcgacg atcgcgtctt tcactttcat cgcgccgtac tgactgttgc ggatggtgtc
    26161 attcaaacca ctgaagtgga cagcgaaggt cgcgccagga ttaaacatct ctgtccagtt
    26221 gatcgcctga acaccgagat agaggtctaa atcgctgtaa accttacact cgcccagcgg
    26281 caacataata cgcgaggcca ggcggctcca catcaggctc tggtaaacaa gccgtgtgtc
    26341 gcccttgaaa tggaccccac cctgaaccac ctggcattca acggccccca ggttttccag
    26401 ttcagttttt aacagctctt ccagcccacg ggccgtactg gcaaacagag aattcatatc
    26461 gtcactttta ctctaagagc ctaccgcagt aggcgtaatt gttgcagtca gtctggacat
    26521 ggacagcgcg gagaaaccgg agcgtacaca agtacgtgag gatttcgagc acttccctgg
    26581 gccaaactga caaataaaat agcctaacgg gacaggcgct aagaaaattg ttgcgcatta
    26641 tagctaataa gcgcgtcatg tcataaagtt gagggcttat tttcatttga ggaccgcacc
    26701 gtggcgacat taacccggct ttttattcat cctgttaaat cgatgcgcgg cattggtctt
    26761 acacataccc tggcagatgt gagtggtctg gccttcgatc gcatctttat gatcacggaa
    26821 cctgatggta cgtttattac tgctcgccag tttccgcaaa tggtacggtt tactccttca
    26881 cctgtgcatg atggcttgca tctcaccgca ccagatggca gtagcgcata tgttcgtttt
    26941 gctgatttcg ccacacaaga cgcaccaacc gaagtttggg gcacacattt taccgcgcga
    27001 attgcgccag gcgcgatcaa caaatggcta agtggatttt tctcccgcga agtgcaatta
    27061 cgctgggtgg ggccacaaat gacccggcgc gtgaaacgcc acaacactgt acctctgtca
    27121 tttgctgatg gctatcctta ccttcttgct aacgaagcct cgttacgtga tctccaacaa
    27181 cgttgtccgg ccagtgtaaa aatggagcaa ttccgcccca atctggtggt ttccggcgcg
    27241 tcagcctggg aagaagatcg ctggaaagtg attcgcattg gtgatgtggt gtttgatgtg
    27301 gttaaacctt gtagccgctg tattttcacc accgtcagcc cagaaaaagg gcaaaaacat
    27361 ccggcaggcg aaccattaaa aacattgcaa tctttccgca ctgcccagga taacggcgat
    27421 gtcgattttg gtcagaattt aattgcccgt aatagcggcg tgattcgcgt tggcgatgag
    27481 gtggaaattc tggcaacggc tccggcaaaa atttacggcg cagctgccgc tgatgatacc
    27541 gccaacatca cgcaacaacc ggacgcaaat gtagatattg actggcaggg acaggcattt
    27601 cgtggaaata accaacaggt gttgctggag caattagaaa atcagggaat tcgtatccct
    27661 tattcttgcc gcgcgggcat ttgtggaagt tgccgtgttc agcttttaga aggcgaagtc
    27721 acgccgctaa aaaaatcagc aatgggcgat gatggcacca ttctttgctg tagctgtgta
    27781 ccgaagactg cacttaagtt ggcgcgttag actgcctgtt cgaggctgaa gctgtcaaca
    27841 ttaacctgcg gtttcagcct gtcgttcatg attttaatgg cgtcgcccaa ctgcatcgcg
    27901 cgacccgcaa taacaacgca gggttgtgcc agcaggcata gtgcggcatt ttcgccagat
    27961 tcaacgacca gcaaattgac ctgctcgtga gtatcgctca accaaacgca ggccgcatcg
    28021 cccggcatgg gcacccacat ttcaccatgg gaaacaaaat gccagctttt cggcatttgc
    28081 ggtttgagat agcgaattgc taccagtgca ttcagcacca gttcggcttt ttgttcttta
    28141 gataaattaa aatcgcggca cttttcttca aaagagaaat agagcgcggc gtcgtcaacg
    28201 caaaagcctg cgggagaaaa ggcatctgga gtcaacatct tgcgcgcaaa acgtgagcga
    28261 aatagcatac cattggctaa atcgagcatc attcgatcgt gctcttcatc gtaataccaa
    28321 cgccagttat cgtctggttt aattcgcatc tgcctctccc ctaccctata aaaccgcagc
    28381 aacacaatcc attgtcgctt cgcttaataa cctcaacaat aaaagtacaa aatgtctaaa
    28441 taagcaacag cgatgaatat aaaacaacca gggctgaaaa taaagccctg attatcttat
    28501 aaagaggaat tcgaagaaat aattagatat gggtaacgat ttctttaatc agcggcggac
    28561 ctttaaaaat aaaaccagaa taaatttgca ccagtgaggc acccgcagca atcttttcac
    28621 gcgcagcgat aaccgagtcg atgccgccaa caccgatgat cggtaagcga ccgtttaatt
    28681 ccagtgacaa gcggcgaata atttcggtgc tttttaactg aagcggacga ccacttaagc
    28741 caccggtttg atcgcaattt ttcattccct gaacaagaga acgatcgagt gtggtattgg
    28801 ttgcaataac gccatcaata ttatggcgaa ctaaactatc ggcaacctgg atcaattctt
    28861 cttcagaaag atccggcgcg atcttcactg cgatcggcac atatttatgg tgcatcgctt
    28921 gcaaatcatt ttgcttattt ttaatcgcgg ttaagagatc atccagcgct tcaccatatt
    28981 gcagcgtgcg taatcctggg gtattcggcg atgaaatatt gatggcgata tatcccgcat
    29041 aggcatagat tttttccata caaatcagat agtcatcttt gccctgctcc actggcgtat
    29101 ctttattttt gccgatgtta atacccagga cgccgtcata atgggccttt tttacgttct
    29161 ctacgaggtt atcaacgcca agattattaa agcccatacg gttgatcaaa ccttcggcat
    29221 ctaccagacg aaagagacgc ggcttgtcat tacctggctg tggacgtggc gtgacggtac
    29281 cgatctcgat cgatccaaat cccatcgcgc ctaacgcgtc aatgcactcc ccgtctttat
    29341 caagaccggc tgccagacca agcggatttt taaacgtcag gcccatgcag ttaacaggtt
    29401 tcgcaggcac tttctgccgc accagtgctt caaacggcgt tcctgtaata cggcgtaatt
    29461 gctgaaaagt aaactcatga gcgcgctctg gatcgagctg gaaaagggct ttacgaacga
    29521 aggggtagta catgaactct cctggattcc cggtgtgcaa accgggggcg tattatgtgc
    29581 gagttcggac caaaagggaa ttgacctgcg gcaaaaaaaa cgcaaacgtt ttccgaacct
    29641 gtttcagggc agattttgct ccttctttgc actcaacccg ccgtaatcat ttatcgcccg
    29701 ccaggcgaca tgacacggtg cgattgcctg acaccagctt gtctgacgct ggcttttagg
    29761 cggcacaact cccgcattac ttaacgcttt accatttacg ctcaactgaa tcaggttaat
    29821 gtagtaaggc gttgggttag tgagttgtac accctgcgaa ttccgtgtcc agcgtaattg
    29881 ttgataagct tccagcgggt cgcccggcaa accttcgggt cgccagaaca gtttaaatac
    29941 cgagcgcatc gccactttta cgctctgatt ttcaactttg ccggatggca cgctggcaat
    30001 gcttaactca aatagcgttt cgcgatccac aggcaagatg tcgctttccg ttctcagcaa
    30061 gcgcaacgtg cctgtagtac cgggcttcag gagaataagt ggcggagcgg ccagtaatgg
    30121 tgctggcact gtcgacgctt cccccccggc ccaacgcgtt gggcggttga ttttactgtt
    30181 aataagccag gattcctgac tggtattagt cagtaaaata gatatcgatt ctctgtctgc
    30241 cggaaagata aatcgcgttc cgccgaccac cactcctgcc cgcaattcac ccgccaccag
    30301 cagcgataaa acggcgaaaa tcaagagcgc caatctgttc cacgtattag tcataatgaa
    30361 gtacaaatgt cgcgctggca ttcactttac ccgactgcac atcgctattg acaggtcgca
    30421 attgcgcata aaaagttaat ggcaccttac cgctatgtag gctgtagtct ttactcgcct
    30481 ggttaagcgg aatcaatatc ttcgcatcat ccagaatcgc aattgccagc ccgctggctg
    30541 catccatacc actttcaatg gacagcattt cgctctgcac tccgtcgggg gttcctgaaa
    30601 aggtgacgtc aaccgtgctg acatctgttc cgcaatcctg caaattaatg atgaaagatt
    30661 tctccgggcc ataattcccg tgagaaaaac gtgtcagcga gacatcgcct aactgcacat
    30721 tgatttgctt gctatcctgt gccagcacgc atgtattacg acgaaacgat ccgctaatgt
    30781 tgaccgtgcc atcatgagca aataactcac tactccccgt taatcccagt aagcacagaa
    30841 tccagattat ccgtttcaac atcactcctt cactggtaaa ccagatcaaa atagagaacc
    30901 gccgtcgcat taccgcccgt tgctcccgcc tttgtggact tataacgaag ttgatatttg
    30961 agtgtgttat ccccggcttt taagggcgta agaggctgga cctgattgag cgggatttct
    31021 tgctgacttt gcgcatcaag aatttgcacg gcaatccccg ttgccatacc accgcttccg
    31081 cctgtgtcag ttagtttaag cagtgtcggc gcaagggtgt cagcctcgcc gctaaaaagg
    31141 acgtccgcgc cagtaatacc agcggcacaa ccggtgatat cgatattcaa atcagctgca
    31201 gtgcttacgg tattcgcggc ctgaaaatca ctgatattaa aatcgccaat atgaatattt
    31261 tgtaatgcac ttttggtggc gacatcacag ccctgatcga ccacattccc ataaatatgc
    31321 atactgactt gttcaggccc agccatcccg acatggatgt taagaaggct aaagaacaaa
    31381 ataacgtact gatatatcgt ttttttcttc atacgttcac gcgttaatca aaatttattc
    31441 tcaccgttgc cgtggcttca aatcgcccgg tttctggtgg tacgcctgtc gtgctgactg
    31501 gatagacatt cattcgcgcg atactgtctg catcatcagg aatagagagc ggtagtgtcc
    31561 caccagcagg ggaaataatg ttgttctggc tatcggtcac caccacgccc acgccaggac
    31621 gtgacgtctt aatcgcctgg ggatagctgg ggtctgaggt tgccgtcaga ctcaggttga
    31681 cgttaactaa cccttcgttt cccgtgttac acctgactgg aattgcgagt tctgccgttc
    31741 tggccccaag gggtttatgc cctacaacac gaaagttagc cgcaaatacg tcgcccagat
    31801 cgacctcgat agtctctccg gcattgacgg aacatgagcc aagtgaattg atcgtaccag
    31861 acaggtaata cactaaaacc ggtgtacctg tagtcgtgca ggaatcacct gcgggtatgt
    31921 tgtagcattc aaataatcgc gccagcgcca taggaggaat aacgaccgaa ccaacgaacg
    31981 gctgcaatat tttcagggaa aagctaccgc tattaccgct agccccctgg ccacctaaac
    32041 gttgaccgcc gggatcgcaa atatgccatc gtgtatctgc ccctgtgccc acgttttcga
    32101 aaggaaccgc agtaggagaa ctgcctttaa cctgcaataa aactttggcg gcaagatagt
    32161 cattaatttt gtaccagttg tcgcctaaat agactaacgg tgaatccgcg gcgaatgccc
    32221 atcggccact tctgacattg tctttatcgc aatcacagga aacataataa tcattcgatt
    32281 cgttccactg ataaaatttg tctttaacta ctccagccgt attttcttct tcctccaggg
    32341 ttttaacgcc aaaattgacg ccaatatacg gattcccctg agtggtatga cactgcccgc
    32401 tataagcagc agtttgagcg caccatagca ttaagacggc ggcaaaaaat agtcgtagta
    32461 ataacacgca tttttctcca aagattatct gcatatggca tccattcgcg taatcgcaga
    32521 gttaatttgt tgttcggtaa gctggtaact gatatgacaa atttgatcgg gagcttcgcc
    32581 ccagcgtaca gttatcgctc cctgagcggg caaaccggac atatagagcg agccatcatc
    32641 atcaataatg ccgctattag tctgtatgcc gtccagcgtc gctatcgcgc caaaacgcag
    32701 cggtataccc tgctttgatg ttttcattaa tacccttgct cccgcatggg cattgaactc
    32761 cgccagaacc aacgcccctt tggtgggaac cacgttgact actgcatttt caagatccac
    32821 gttacgtttt aacgatgccg catcaagtgc gattcgattt tcccgataag aagtggcata
    32881 ggggacaacg gtatatccgc gccagtcagt tttcagcccg gtgctgttcg ccacacgagt
    32941 attctctgcg cctggtgctg caatcagcac gttagtttcc cccagcgatt gccccagggt
    33001 aatgccctgt gaatgggcaa ctaaactgcc tgaaagagcg tagttcagtt gttgttgact
    33061 gccgttatcg ctgtagttgt agcccactcg ggcatctgca aacgcccctt tgtagtccat
    33121 gctggcgcta ccattagccg ttttcccctc gctgttatat ccctgttgca cgctgtagct
    33181 caggttaccg tcatcgagta gtgtctccgt taaacccagg ttttgtgtaa tgtgcccttc
    33241 gttatcgata ctggtgttag aggtcatcca ggcgtaattt tttttcctgg tatagctatc
    33301 attacttctg gggagcaaca gattgagcgg cagggaaaaa tttagtgcaa acacctgatc
    33361 agcatcaggt tgaccacggg acttactgta gttccaggaa acccccagcg agagatcttt
    33421 aatttgcgtg ttgtagccaa actgtaatag ccgatcctgt tgatcggtat gccagtaagt
    33481 ttgctggcta ccacttaaat aaaacgaacc gtactcgcct aattgctggg agatattgac
    33541 ctgcagtttg ccacgtttgg tgtaaaacaa attgtaataa cgcgaccaca tcggcgtatc
    33601 ttcatcatca ccgtcattaa attcgtagcc atccatatgt ttatacatgg tgtcggaaag
    33661 ggtgtagaaa cccgacgtcg aatagcgata gcccatcaat tgaaaattag tgcccaattg
    33721 gttgagcgtt ttggcataca gaaaacgata tgactgacct ttatgttcgc tttggtctgc
    33781 cagcgtactc ttcgcctggg tcgcatcgaa cgaaattgct ccgaaatcgc caaggttaaa
    33841 tcccagacca aacatggcgg cacggtaata ttcagcatat tgtccaccac cataccatgt
    33901 cgttccccac ggtccgcccc actgcaacgt ggcctgggca aatttgctct cctgctgttc
    33961 attactattg gttctgtatt tcgccagtgt caccgcgtat ttgattcgcc cctgacgctg
    34021 gagtaatggc acgctggaaa acggtacgct gtagctattg acgctgccgt ccgcttcttt
    34081 gatttcaact aacaaatcac cgctcgacga cgtggaatag agatcactaa tttcaaaagc
    34141 accaggcgat acataagttt ggtaaatggt gtaaccattt tgcttaatcg ttatctgggc
    34201 attgcttttg gcgataccac gcaccacagg cgcaaaccct ttcaggctgt ctggcagcat
    34261 gttgtcatct gacgccagct gcacaccacg aaagctgaca ctatcgaaaa aatcgcctgc
    34321 agtataatca tcacctacgg tcagttcgcc ttttagtgga ataaccgccc gctgcaaata
    34381 gctgctgaga ttcttccatt ctgcggtttg gccactactg cgactccatg ttgaattgtt
    34441 gcgcaatctc catgggccta aattaacgcc actgtttaaa ttcagaaaat agctgtcgcc
    34501 agaatcactg tctgcgctgc tatgaatact gttagccccg ctaaacgaat atcccagtaa
    34561 tagcgcgttg atcccttcat cccaacgttc aggggggaca taaccacggg cattactttt
    34621 gatggcgatt tgcggtactg aaatttccag acggagttta ttcacattaa acgttacgct
    34681 ggcatccgga ataatctccg ccaatggcac acacgcttgt ttgtccagtt gtttaaaagc
    34741 cggaaacgca tcagtattaa cacccattgc atccaggctt tcagtcgtca aacaggcggc
    34801 aatccccttc tctggcgtta tctcaacaaa attgacgttg cgcgtatcta caattgtctg
    34861 attcagaaca atatcgacac gataaacccc tggcgttatt ttttgtcctt tttcgaaacg
    34921 agacaaatcg gccaccattg agggatcatc tttgaaaaaa gcaggatcga accaggtttc
    34981 agcttgtgca ttgaacgctg ccgacacaaa caccaccagc ccaccaataa acgatggcac
    35041 tccaccagag cttaacaggc tgtgttgtcg atgagttcta tacatctcgc tctcttaact
    35101 gaagacggct tacgatttac agttgagttg cggcgtttcc gccccgtagt cattgatggt
    35161 tcgatacgtt acgctgctat ttttggcccc ctttggcaga gaaaattggt atttatcaaa
    35221 gggggaaatc atttgatttt taactacttt tccacctatt ttcaggtctg tcagggtaat
    35281 gaaataaggt gttgggttac tgaccgtaac catgtcagct gacgaagtgc aacgcagttt
    35341 cgccggagcc tcgcctaagg gatacgctaa accttttggt cgccagaaaa gtttgatgac
    35401 gctttgtgtg gcaatcttca gggtatttcc ctcttcccgc ttcgttgttg aagggactgc
    35461 ccgtacgcta gtgaagaaaa gggtttctct gtctttcgcc aacggcgcac caatgtacat
    35521 aatgcgtaac agattctcat tagccggatt cagcacaaat agcggtggtg taataataaa
    35581 atctgctgat tttttattat ctggggtcat cacccatgac tgaataagat aaggaacatc
    35641 cggactggtg ttacgtacac ccagtgaaat ttgctcttca ccttctgaat aaacaagacg
    35701 tgttgctgca agtccaatcc cgcctttacc tgccgcccat gcggatgagc agctgagtaa
    35761 tattgccgtt aaacttacgg tcacaattcc ctttgttatg caagttttca taaatagtca
    35821 ctcttaaaaa atatgaatat cccctccaga acactagagg ggtactaatt aataaaacaa
    35881 ttaatgagtt ctattaataa tagttaatat ggaaatttgt taccgcattc acattaccag
    35941 aagtcgcttt ccccgtagcg atataatcaa ctttaaaggt cactgtttgc tcagcagaat
    36001 ccgctaactg aatattcgac gtttcggtac cgggagttac cgatgtagaa ccatctggca
    36061 gatacatttg tagagcaaca ttcgtcgctg catcggtggt agcctggttg gcaaatgctg
    36121 taggttgcac gccgtcagcc gtaccactaa aggtaaacgt cgcattttta gttacagtaa
    36181 catcacagcc ttctaaggca attggtacat cagttttaac gccagagact gctccattag
    36241 tttgcaactt ggtggcgctg acatttggca atgtgactac tgaatctttc ttcgcagcta
    36301 ccagggtaca agctggagca ataactttac cattaaatgt tactttgcca tccgtgatag
    36361 cattatcatc agctgccata acgctggacg ttgcacatac cacagttata aacgccgtca
    36421 atacactttt tttcatcgtc atcatcctga tctcttcgta
//
LOCUS       DAZGJW010000033        35589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZGJW010000033 DAZGJW010000000
VERSION     DAZGJW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.033"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..802)
                     /locus_tag="ACHKIK_001694"
     CDS             complement(<1..802)
                     /locus_tag="ACHKIK_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO9082886.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQKITELIEVVYNRRVDRSSFNYSRFMMHLKYFSSRVLCNEK
                     IKQKNIGDIYEQFLEKDFQLQRAIHEIERYLYATFKYDSLLEEKLYLSI"
     gene            complement(1062..2315)
                     /gene="amiC"
                     /locus_tag="ACHKIK_001695"
     CDS             complement(1062..2315)
                     /gene="amiC"
                     /locus_tag="ACHKIK_001695"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO9082887.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2547..3878
                     /gene="argA"
                     /locus_tag="ACHKIK_001696"
     CDS             2547..3878
                     /gene="argA"
                     /locus_tag="ACHKIK_001696"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO9082888.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3940..5766)
                     /gene="recD"
                     /locus_tag="ACHKIK_001697"
     CDS             complement(3940..5766)
                     /gene="recD"
                     /locus_tag="ACHKIK_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO9082889.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMVL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVVSGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(5766..9308)
                     /gene="recB"
                     /locus_tag="ACHKIK_001698"
     CDS             complement(5766..9308)
                     /gene="recB"
                     /locus_tag="ACHKIK_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO9082890.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVELAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEELELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(9301..12189)
                     /gene="ptrA"
                     /locus_tag="ACHKIK_001699"
     CDS             complement(9301..12189)
                     /gene="ptrA"
                     /locus_tag="ACHKIK_001699"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO9082891.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSDKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQHLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEDQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12365..15733)
                     /gene="recC"
                     /locus_tag="ACHKIK_001700"
     CDS             complement(12365..15733)
                     /gene="recC"
                     /locus_tag="ACHKIK_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO9082892.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLADDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSVEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPDDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15746..16069)
                     /locus_tag="ACHKIK_001701"
     CDS             complement(15746..16069)
                     /locus_tag="ACHKIK_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO9082893.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(16054..16461)
                     /locus_tag="ACHKIK_001702"
     CDS             complement(16054..16461)
                     /locus_tag="ACHKIK_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO9082894.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16458..17021)
                     /locus_tag="ACHKIK_001703"
     CDS             complement(16458..17021)
                     /locus_tag="ACHKIK_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO9082895.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(17012..17482)
                     /locus_tag="ACHKIK_001704"
     CDS             complement(17012..17482)
                     /locus_tag="ACHKIK_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO9082896.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17666..18460)
                     /gene="thyA"
                     /locus_tag="ACHKIK_001705"
     CDS             complement(17666..18460)
                     /gene="thyA"
                     /locus_tag="ACHKIK_001705"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO9082897.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18467..19342)
                     /gene="lgt"
                     /locus_tag="ACHKIK_001706"
     CDS             complement(18467..19342)
                     /gene="lgt"
                     /locus_tag="ACHKIK_001706"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO9082898.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19493..21739)
                     /gene="ptsP"
                     /locus_tag="ACHKIK_001707"
     CDS             complement(19493..21739)
                     /gene="ptsP"
                     /locus_tag="ACHKIK_001707"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO9082899.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21752..22282)
                     /gene="rppH"
                     /locus_tag="ACHKIK_001708"
     CDS             complement(21752..22282)
                     /gene="rppH"
                     /locus_tag="ACHKIK_001708"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO9082900.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(22635..22781)
                     /gene="ygdT"
                     /locus_tag="ACHKIK_001709"
     CDS             complement(22635..22781)
                     /gene="ygdT"
                     /locus_tag="ACHKIK_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO9082901.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            22967..23656
                     /gene="mutH"
                     /locus_tag="ACHKIK_001710"
     CDS             22967..23656
                     /gene="mutH"
                     /locus_tag="ACHKIK_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO9082902.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23725..24438
                     /locus_tag="ACHKIK_001711"
     CDS             23725..24438
                     /locus_tag="ACHKIK_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO9082903.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            24576..24794
                     /gene="ygdR"
                     /locus_tag="ACHKIK_001712"
     CDS             24576..24794
                     /gene="ygdR"
                     /locus_tag="ACHKIK_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO9082904.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24902..25942
                     /locus_tag="ACHKIK_001713"
     CDS             24902..25942
                     /locus_tag="ACHKIK_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO9082905.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25974..27167)
                     /gene="lplT"
                     /locus_tag="ACHKIK_001714"
     CDS             complement(25974..27167)
                     /gene="lplT"
                     /locus_tag="ACHKIK_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO9082906.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(27160..29319)
                     /gene="aas"
                     /locus_tag="ACHKIK_001715"
     CDS             complement(27160..29319)
                     /gene="aas"
                     /locus_tag="ACHKIK_001715"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO9082907.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            29905..30936
                     /gene="galR"
                     /locus_tag="ACHKIK_001716"
     CDS             29905..30936
                     /gene="galR"
                     /locus_tag="ACHKIK_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO9082908.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30943..32205)
                     /gene="lysA"
                     /locus_tag="ACHKIK_001717"
     CDS             complement(30943..32205)
                     /gene="lysA"
                     /locus_tag="ACHKIK_001717"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO9082909.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            32327..33262
                     /gene="lysR"
                     /locus_tag="ACHKIK_001718"
     CDS             32327..33262
                     /gene="lysR"
                     /locus_tag="ACHKIK_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO9082910.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(33249..33941)
                     /gene="ygeA"
                     /locus_tag="ACHKIK_001719"
     CDS             complement(33249..33941)
                     /gene="ygeA"
                     /locus_tag="ACHKIK_001719"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO9082911.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(34070..35488)
                     /gene="araE"
                     /locus_tag="ACHKIK_001720"
     CDS             complement(34070..35488)
                     /gene="araE"
                     /locus_tag="ACHKIK_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO9082912.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8790 a         9706 c         8712 g         8381 t
ORIGIN      
        1 gaatagaaag atatagtttc tcttccagaa gagaatcata tttaaaagtt gcgtacagat
       61 atctttcaat ttcatgaatg gcacgttgta actgaaaatc tttttcaaga aactgctcat
      121 agatatcacc aatatttttc tgttttattt tttcattaca taacacacga ctagaaaaat
      181 atttaaggtg catcataaat ctggagtagt taaatgaact tctgtccacc cttctgttat
      241 aaacaacttc aataagctcc gtgatttttt gagacagctc aataatcctt actgattcat
      301 aatcaactga attttcataa ttatttaaaa tatgcaatgc tataaaacca atctcatcat
      361 ccggtagttg aacgctaaat tgctcattaa tgattgttaa tgcatcacga cttattgcat
      421 actctgcttt gtacaacgct ttgatttcct caaggaaaac attattaatt tgtatgcctg
      481 agacgactcg ggatattgca ttcgaaatgt gatcagcaag aatcagtagt agcgatggat
      541 tgagtttttt ttcgagatta ctctccgcat aatgcactat cttttcggca acaaaaatat
      601 actcaatacc cacatgctca atcaatttgt agagtttatt tttttgctca tttctgacat
      661 aaaaaatccg gtttgcagga tgctcaggga ccgacatacc atatttttta ttgtaaccaa
      721 cgccaggccc agaaatgata acctcctgtc cgttcaccga tgcctgtaca caattattgt
      781 tcatgacttt ttcgatgatc attccatggc tcccaaaaaa aaaggcaagg catgtcagaa
      841 gtaataatta cttcaaacat tgccttgcct gatttaacag ttacaagata attaactaat
      901 gcgtatacct tacacggaat tgtgcttaat aaaagagatg acaggcttat tttttaagcc
      961 gatattcaaa tattcgacag cagtcacaaa accatcgttc aacatttatg taaataatat
     1021 ttattcaatg aaacataaat taattgaata aattaaaaac atcatcccct tctcgccagc
     1081 gtcgccccat cggcaaaata cgctttaatc cccgcaagaa tagactccgc aacttcctgc
     1141 tgaaaggtcg ccgttttcag tttacgctct tcctcaacgt tactgataaa cgccgtttcg
     1201 accagaatgg agggaatatc tggtgccttt agtacggcaa acccggcctg ttcaacctga
     1261 tttttatgca gcttgttgat tttaccgagc ttattcagta ccgctttacc aaacttaagg
     1321 ctgtcagcaa tggtcagcga ctgcaccata tcgaacatgg tgtggtcgac atagcggtca
     1381 ccgcttttgc tcacgccacc aatcaagtcc gaggcgttct gggtttgtgc cagatatttt
     1441 gccgcagtac tggttgcacc tttggttgag agcgcaaaca cagaggaacc gctcggctga
     1501 cgactggtaa aggcgtcggc atggatagag acaaacaggt cagcacgctg tttctgggct
     1561 tttgctacgc gcacttgcaa cggaatgaag atgtcttcat tgcgcgtcat gtacaccttc
     1621 atattgccct ctttctcgat cagagagcgc aaacggcgag ctatttgcaa taccacatct
     1681 ttttctcgcg ttttgtattt ccccaccgca ccggagtctt cgccaccgtg gccagggtca
     1741 agcataatga caatcggacg atcgcgccct gctttacccg gttgtggacc actttgtgct
     1801 ggcggcacct gcttttcgag gtcgcctttg ttgtaatcct ccagcagcgc cagcagcggg
     1861 tcctgcatat cctgtgcatt ggccggatag aggtccatca ccagacgctc tttaaacccg
     1921 gcgaccggcg caagggcaaa cagctgcggt tttacgtttt gctttaattc aaaaaccatc
     1981 cgtacggtct gcgggtcaaa ttgcccgacg cgcgccgact tgatgaacgg gtcgtctgca
     2041 cgaatttgtg ccgccatccc cttgagtacc gagttcaggt ttacatcttc gatatccacc
     2101 accacgcgtt ccgggttact caacgcgaac tgcttatatt tcagctgacg atttgattct
     2161 accgtcacac gggtgtagct ggacgcaggc cagacgcgca ccgccacgac ctggctgacc
     2221 gcagccagac tgacctgact tacgctcaat agccacatgg cacccgcgcc ttgcagtaaa
     2281 cgacgacggc tgattgcagt gttggatcct gacatgcctc tcccgagcaa aagaaatcta
     2341 agctgtgtaa caagtaaacg actaatttga ccggaaactt tagcgaaaga cgcataatct
     2401 gtcatctaat aaacggtaaa cattcttttt atattcacgg cattactgat aaaaaagtcg
     2461 ctctcgcata aaatttacac ttgcaccctg cgaaaaaaca gaataaaaat acactaattt
     2521 cgaataatca tgcaaagggg tgtgccgtgg taaaggaacg taaaaccgag ctggtcgagg
     2581 gattccgcca ttcggttccc tatatcaata cccaccgggg aaaaacgttt gtcatcatgc
     2641 tcggcggtga agccattgag catgagaatt tctccagtat cgttaatgat atcgggttgt
     2701 tgcacagcct cggcatccgt ctggtggtgg tctatggcgc acgtccgcag atcgacgcaa
     2761 atctggccgc gcatcaccac gaaccgctgt atcacaagaa tatacgtgtg accgacgcca
     2821 aaacactgga actggtgaag caggctgcgg gaacattgca actggacatc actgctcgcc
     2881 tgtcgatgag tctcaataac acgccgctac agggcgcgca tatcaacgtc gtcagtggca
     2941 attttattat tgcccagccg ctgggcgtcg atgacggcgt ggattactgc catagcgggc
     3001 gtatccggcg gattgatgaa gacgcgatcc atcgtcaact ggacagcggt gcaatagtgc
     3061 taatggggcc ggtcgctgtt tcagtcactg gcgagagctt taatctgacc tcggaagaga
     3121 ttgccactca actggccatc aaactgaaag ctgaaaagat gattggtttt tgctcttccc
     3181 aaggcgtcac taatgacgac ggtgatattg tctccgaact tttccctaac gaagcgcaag
     3241 cacgggtaga agcccaggaa gagaaaggcg attacaactc cggtacggtg cgctttttgc
     3301 gtggcgcagt gaaagcctgc cgcagcggcg tgcgtcgctg tcatttaatc agttatcagg
     3361 aagatggcgc gctgttgcaa gagttgttct cacgcgacgg tatcggtacg cagattgtga
     3421 tggaaagcgc cgagcagatt cgtcgcgcaa caatcaacga tattggcggt attctggagt
     3481 tgattcgccc gctggagcag caaggtattc tggtgcgccg ttctcgcgag cagctggaga
     3541 tggaaatcga caaattcacc attattcagc gcgataacac gactattgcc tgcgccgcgc
     3601 tctatccgtt cccggaagag aagattgggg aaatggcctg tgtggcagtt cacccggatt
     3661 accgcagttc atcacggggc gaggttctgc tggaacgcat tgccgctcag gcgaagcaga
     3721 gcggcttaag caaattattt gtgctgacca cgcgcagtat tcactggttc caggaacgtg
     3781 gatttacccc agtggatatt gatttactgc ccgagagcaa aaagcagttg tacaactacc
     3841 agcgtaaatc caaagtgttg atggcggatt tggggtaagc gatgaaaatc gccggatgcg
     3901 acatgcgtaa cactcgtgcg tcgcatcagg caattacgtt tattcccgcg aactaaacaa
     3961 tgccgccaga ccactgcgcc gctcagtgcg cgtggcgatt gccgcactta atatgcgctc
     4021 atcggcatac agcgacagac ggcgacgcgc gcgggtcact gcggtgtaaa ccagctctcg
     4081 cgttactacc ggcgtgcgtt ggctcggtaa aatcaacgcc gcatggtcga actccgatcc
     4141 ctgcgattta tgtaccgtca tcgcccacgt cgtttcgtgc tctggcaggc ggctcggttg
     4201 cacagactta atattgccgt ccggcatcgc aaaccaaacg cgcgtcccct gcccgcgatc
     4261 cagcgcaata ccgatatcgc cattaaacaa cccaagcgcg ctgtcattac gggcaatcat
     4321 caccggtcga ccttcgtacc aacgagagtg cggatgacga tgaattttgc gcttctgttg
     4381 cataaactgc tcaattcgct cattcagtcc agccacgcca aacggccctt cccgcagggc
     4441 gcacaaaagt tggtactcat tgaacgcctg aatgattaaa tccggctcgg cacgcgcttg
     4501 cagcagatcc agataacgtc cataacccgc aagagcttcc tcaagcatcg caatataatc
     4561 ttctccgctc tgtaaaagtc gtttttcgat atcagtaaaa tcctgctgaa aaacggtttt
     4621 cactgccgtt ttatcaccac ggttgatcgc cgcagctaac tgaccaatgc cagaatcgct
     4681 gccgaaacga tagctttttt gcagcaggca gagactgtcg cgcaaagatg ccgcttctgt
     4741 gccggttcct gccggaacgt gagtccccgt caggcggctt agctgcctgg cacgctcggc
     4801 ggtaaagccc gcgttggcat aagcgcagat atcgcccagc acagccccag cctcaaccga
     4861 ggccagttga tcacgatcgc cgagaaagat cactcgcgca tgatctggca aggcgtcgat
     4921 cagtctcgac atcataggca gatcgatcat tgacgcttca tctaccacca gcacatcaag
     4981 atgcagcggg ttaccggcat gatgacgtaa acgctggcta cccggctgcg cgcccagcaa
     5041 tcggtgcaaa gtgctggcat cttccggaat gcgtttcttt tgttcatcgg tcagcggtaa
     5101 ctgtcgcaaa gccttgccga gagattcggt taagcgcgcg gcagctttac ccgttggtgc
     5161 agccagacgg atacggcagc gttcgccgtc ggccatttga attaacgctg ccagcaactt
     5221 cgccacggtg gtcgttttac cggtgcctgg gccgccggaa accaccgaga tccgccgcgt
     5281 cagcgccact gccgctgcaa ctttttgcca gttaatttca tcgcttactg gaaaaagttt
     5341 gtccagggtt tgcgccagta gagcttcatc aacctcaatg gcatgattca cctcattgaa
     5401 gaagcgcgct accgtccgct cgttacacca catgcgattc aaataaagac gatcgccaca
     5461 gaggaccatc ggcgtgggtt catctcccct gctgaccgct tgagaagcca gcaagcattc
     5521 ttcccaattt tgtagctcac cgatttcact gacacaggtc gccaacagcg gatgcgacgc
     5581 ctcgttattt tccagtcgtg aaagcggcaa acagacgtgt ccctctccgg catcatgact
     5641 taacagtgcc gccgcgaggg tgacggcagg atgttcatct cccgccacgg tcagggcaaa
     5701 ttgcacatcc agcgggcgta gctgtttgtg ctccacagct tccagtaatt gcttttgcaa
     5761 tttcattacg cctcctccag ggtcataccg gcaaacatct catccatcag ggcaatcaac
     5821 ccggcgttgg gtcgggttgt gtaaatccct tgttgcggat gttctttatc aacgccacgc
     5881 aggaacagat agataacgcc gccaaagtga cgatcatagt cgtaatcagc aatgcgatgg
     5941 cgcagataac gatgcagcgc cagggtataa agctgatatt gcagatcata gcggtgtgcc
     6001 tgcatcgctg ctgccatagc ctgttgggtg taagccgaac tgtcttcacc cagccagttg
     6061 gatttatagt cgagcaggta ataacgccct tcgtggcgga ataccaggtc gataaagcct
     6121 tttaacatgc cgcgtacctg catgaactcc agcggcgggc agccagcgga tagcgggtca
     6181 aactggcgaa ttagtgcatc aagctggctg gcgatgagcg gttcgctaat cggcaggtaa
     6241 aactccatct ccacctgttt atcgcggtcg gaaagctgac tcaggctaac acccgtttca
     6301 ttgagaggtg cctggaggac agccgtgatc cactcggtca acaccggttc ccactgcgat
     6361 tcaaagccgc cgagttccag ttcttcctgt acccagttcg ggtcaaccgg ctgggtaaaa
     6421 tcgaggtctt caaacaaact gtgcaagaat gtccccggtg acgcaccgcg cgggaactga
     6481 tgcggtgtta acgtcggttc ttcaacgacg ctgaccacgc ctgcggcatc aacatccagc
     6541 cgtggcatca gatcctgagc gataccgtga ccacgctgct gcaagccgga gtagctggtg
     6601 acacgccagt tatcgccggg caatcgttgt agcgtcctcg cattcagttc tgcagttaaa
     6661 gcatcattaa cctgccaggg ctggttatca ccagtttgtg ccgtttgcca ggcaatatca
     6721 tcatcgcata acgcttcaat acaggtgcga agccctgccg catcttgcgg ttcccctttt
     6781 tgcagcaaac gcccgagcgc actttggtgg acgtcggtgt cacctttttt atcgccacga
     6841 cggcgcacca gcggtgcaac gccgagactg caatgccaaa ccgaacgcgt cagcgccacg
     6901 taaagcaaac gcagatcttc cgccagacgt tcgacctccg caagctcgac gctttctggc
     6961 gcagcgttaa gatccagaac agcctcaaac gagtggcgat cgtgataaaa cgcctgatcc
     7021 tggacgcgga aattggtaat aaatggcaac cagaccagcg gatattccag ccctttcgat
     7081 ttatggatcg tgacaatctg caccagatgt ttatcacttt cgaggcgcat ttgttggctg
     7141 gaggcattac tgtctggctc gaggatatgt tgcgataacc agcgtaccag cgcatgttca
     7201 ctttccagct gcgttccggc ttcttgtagc agttcgctga tatgcaagat atcggtaaga
     7261 cgccgctcac cgcctgccgt tgccagcaag ttttctgcaa tgttacgcgc cgacatcagc
     7321 gcccgcagca tcggcataac gccacgtttg cgccagattt gccgataacc atcgaactct
     7381 tcaactaccg catcccacgc atgttcgtca ttattcagcg tttcgatatc cagcgcgttc
     7441 agccccatca ttgacgttgc cagcgcactg cgcagggtgt tctcacgttc gggcgtcatc
     7501 accgcctgca acaaccaaag catttcctgc gcttccagag tttcaaaaac actgtcgcgg
     7561 ttcgaaaggt aaacggaagg gatttccagc aacgttaagg catcgcgcac ctgggcggcc
     7621 tcctggcggc tgcgcaccag cacactgatg tccgaagcac gcaccggacg cgcgtcgtcg
     7681 ccgttcatca gcaacgcttc gccccgctgt ccggcttgta gccagtcgcg gatttgcgca
     7741 gcacatacct gcgccatggt actttgataa tcgccaacgc cgcagctttc gccttccatc
     7801 agccacattt tcatcgcagg ctgcgtttca cctttaaaca caaaacgtaa cgcctgattt
     7861 ttcccggctg atttcactgg aataaacggt atttcgcgaa acatgaacgc gtcatcagtc
     7921 tggctgaaaa gcttgttcac actgttcacc attccaggtg cggagcgcca gttggtgtct
     7981 aaagtgtagt gggcgtgaac ttcactacgc gccttcatat aagtgaagat atccgcaccc
     8041 cggaatgcat atatggcctg cttcgggtcg ccaatcagca acaatgcggt ttccggctga
     8101 tggtgccaga tacggcgaaa aattcggtac tgctgggggt cggtatcctg aaattcatcg
     8161 atcattgcca ccgggaatcg cgtacggatc gccgctgcca acacctcacc gctttcgcta
     8221 cgcagcgcgg aatcgagccg acttaacatg tcatcaaaac ccaattcgcc acggcggcgt
     8281 ttttcacgcg ctactgtttc gcggatctca gccaatgcgc gggtgatcac cagatcgcgg
     8341 atcgacaatg gttctgcaag cagttgatcg atcgcctcaa acagtggatg tcgcggggtt
     8401 tcccccccgg ccttcgtgcg atcttctaag aaacgctggg agaatttttc cagcgactcc
     8461 ggcaactgat aactgtttgt ctcttcttct gcccaggcgc tgatcttttc gatccattta
     8521 gcctgattgc tacggttaaa cttgcgtcga tcaataccag aagattcgat cagcgcatca
     8581 agttcaccca ctgcgtcgcg ccactgctgt tttaccgtat caatacgcgc cacaatttgc
     8641 gcgtggcggg aagccagcgt ttcatcatcg ggcggcggtg ctttgataac cggtgcttcg
     8701 ccttgcagat aacgattaat atcgcgcagc aacgcctgcg gccctttcca ggtttcaaag
     8761 accaccaggg caatttcacg cggcagcggg tagcagtggc gacgccagaa atcggcgcag
     8821 gcctggtagc gtagcagaga ctcatcttca atcagctgct gctcaaacag cataccggat
     8881 tcaaaggcat tcaggttgag catgcgctgg caaaaaccgt gaatagtaaa gactgccgct
     8941 tcatccatct gccgttcggc taacaacaac cactgcgcgg cttgcgcttt atcgtcgatc
     9001 tcttccagca ggcgttcgta cagtggattg tcggtggttt cacgcagaca ggcgatgcgc
     9061 aactcgtgga tattgctacg gatacgacca cgcaattctg ccgtggcagc ctcggtaaag
     9121 gtgaccacca gcagttcttc aacggtcagc gggcggggaa aggcggcgga accgcctagt
     9181 ccaagtaaca ggcgcaaata gagcgccgca atcgtaaagg ttttgcctgt gccggcagag
     9241 gcttcaatca ggcgctcgcc ctgtaagggc aagcgcaaag gatctagtgt ctcggcgaca
     9301 tcactcattc ttttcactca tcaggggcat tgtttgctgc aacgcgctga cgttctccca
     9361 cactttccag ccttcaggat gcacatattc ggctttcccg ttctggctgc cggaaatctg
     9421 cgacagaata gccatgcctt gcggctcgac caccgcctga tggaagaaat cagcaagttt
     9481 ttgcggcgtc agcagtttta tctgggccac gattttatca cgcgaatcga agcgcatatt
     9541 gccgcgatcg aaatctttac ttaacttcga tgcttcttcg ccgagcgttt gcggtgcctg
     9601 cagcatctgg gtaattaccg cctgctggat ttgcgcaaac tcttctggct tcatcgcccg
     9661 caattttgcc tctgcggttg ggaaaaacgc cttgtaacgc tcccacaaga aggaaggctg
     9721 tttatcattg ctttgcaaaa ggaagcccat gccccactga cgccccacgc tcattggaaa
     9781 tgcaaacacg gcatagccca attgttcttc ggtacgcaac tgattgtaga accacggctg
     9841 tacgatctgc cccaacagag agctataggc tgagctggtg tattcatcgt agccagtcgg
     9901 tacaaatacc gctgccagtg cggagtcggt gctgttacca gctttttcaa agatgacgga
     9961 ttgtttttta tcgaccacga catctttgtt acgacaccac tctgaaccat cagcgcccaa
    10021 ctgtttttgc acatcgcgtg ccagcgttgt tgcctggtcc tcggtcatgt tgccgataac
    10081 cataaactct ggtcgagccc ctgattttaa ggcgtcgcga taggccagca cctctttcaa
    10141 cgtaatggag ggcaaaattt tgcgccgttc atctcgcgag aagtacggca cttgcgagag
    10201 catctgcgcg ggcataatcg cctgctcgaa cgctttgccc ttttctgcgg aatccatcat
    10261 ctggttatac caggacttcg cctgctcaag ctgatcttcc gtggcggtat agctaaagta
    10321 gccttccagc aatgcctgga acagctgcgg cagatgctgg gtgtaaccat tagcattaac
    10381 cataaggccg ttgttagcgt tggtggaaaa acttatgcca ccaaccgacg cctggttgct
    10441 taactgatca agcgccagcc ctgcgagata atcattgagc gcaaacatca cctgattgcg
    10501 ggcgctgtcc atggctttcg gattacgcaa aatcaggctg acatcggctt ttggctcgct
    10561 ggcaaaataa cggcttggcg catacaccac gcgcagattc gactcatcaa caatcagctc
    10621 tggatggtca tatttcttgt ctgacttaat cagcgagaaa tcgtcaggaa tataagggtt
    10681 aagctctggc aaagagagcg caatgtcggc ggcttttttc tgccagtcgg cgaaagtttg
    10741 tgcgctgatt ttatcgacct gatacggcgc atcgacaaag taagccgttt tgttgtgcgg
    10801 ctctttcggg ctgatatacc agatacgcgc attctgcggc gtcatcatcg ccagacgttc
    10861 ctttactgct ttagcatcgt accgatcggc aatattgact gcatccagcg tatgctcaac
    10921 aggaacgcga atcatggtat ctgccagcca ttcgacgtaa tccatatcac gggtgattga
    10981 cggataacgg aagtcgatat ccagcacatt cgccagttca tcgaagtatt gtttatcgat
    11041 ccctttttca cgtaacaaat tgaggtagct aaaaatagcc gccacaacct gatcgcgatt
    11101 cgccagacct ttatcggtta aagacgcaga gatcgctaat acgccgctgt tgccgttgac
    11161 gataggatcg gagttggcgc taatgccctc aactaatccc tgcttttgca gccagtcgga
    11221 aagtgtacct gggctgcgat taccaatcag ataggtaatc aattcatcgg ttttgctacg
    11281 gaatttcgcc gagttgttat caatacgaaa ctctacgcgc aacactttac gcggcagcgc
    11341 cgggacgtaa tgaatgataa tgcccttttg cgcgtcggtg actaccggca cggtgatttc
    11401 cggttttttg ctctctttgt tcggcacgcg accaaaggtg tccgccgcca tttttgccaa
    11461 ctccggcagc ggtttattgc tgtaaataac cgccttcatc agattggcgg aatagtactt
    11521 ctcgtggaaa tctttcagcg cctgctgcac cggattacca ggtttgtcgc ttaaagtttc
    11581 gaggttacca ccagaaaact ttgaaccggg gtgtgccggg ttaatggttt ctgcgctgac
    11641 ctgtgccatg cgcatcccgt cacgcgtacg cgccatggtt aattcagcgt tcactgcatt
    11701 acgctcacgt tcggcgtatt tcttgtcgag caagggttct gcaatagcat cagccaggcg
    11761 gtctaccgca ccaggcaagg cgtcgttctc aacttccaga tagaaagccg tgcgatacgg
    11821 cgcagtgctg gcattgtgac taccgccgtg cattttgaga tattcggcca gactgtcagc
    11881 ctgcgggtac tttttcgacc ccatcagact catatgttca aggtaatgtg ccagcccctg
    11941 gtacgcctcg ggatcttcca gcgacccaac gggcaccacc agcgccgaga gcgatttaac
    12001 tgcctgcgga tcagaaacca gcaagaccac cataccgtta tccagacgta tagcctgata
    12061 ctggcggtta tctttatcac ttttacggat ggtttcctga atcggctgcc atcccgtttc
    12121 tgcctgactt aagggtgccc aaagggcaac taacaacaat aatgctttga accaggtgct
    12181 gcggggcatt cacggacctc ataatcaact taattttctg tccagattca acacgttaac
    12241 gcaaatcatc tgccagaatt taatcttgtg ctgcacgagt cagcctatgt ttatataacc
    12301 atcagtccgt gactggtgcg catcataaag taagcggata gattgcgcaa tttttataca
    12361 gcactcatga ctgattaaag cgaaacagcg gtaacaggaa acgttgcgac tgttcaacga
    12421 tggcctccat tgtctctggt gttaattgcc gccagagcct ttgataccag atatcatcac
    12481 cttcgccacg caccatcatg ttgccttcgt aagcctgaag gaatttcgta cgggcttttt
    12541 gcaacgtgga atcgtcatcc agcatggcat cgttttgcgc gtcataacag gtttttagcc
    12601 acgcgccgcc actttcaggt aacaccagca atggcgcgga catcccttca cgatacccct
    12661 caatcagttg tgagaggtaa tgcaaagcct gttcggctgc aagcggcgga aaacgccact
    12721 cgccgtcttt gcgtagaaaa aggcgacttt caccattacc accgctggca cagtagacaa
    12781 ggtgttccag ccaaagttgc attccctgcg ccacacttaa taaagaggga cgccagcgca
    12841 acaggccatc cggctgcacc tgcggcaacc agccagttat ctgcacaccg ttgcaggcga
    12901 gatcaatttc catactctgc cccggctggc gacaggcaat gactctgtcg gcaagctgct
    12961 gcatctcctg gcactgtgtt tcccagaaaa tttcaccaaa agcgccatac ggtaaatccc
    13021 ctgccgcccg gaagcggcgg aacaagcgtt cggcatcatc ctgctcaacc agtgcattca
    13081 ataactgctg attgatttga taacggctaa gcccttccag aataaatggc tcggtgtctg
    13141 ggatttcgct gtcttcagta cggaagttca cctgcaaacg catctggaag aacgcccgca
    13201 ccggatgtgc ccagaatcgt tgtagcgttt ccagcggcac ggtttccggt aaggtaaacg
    13261 gcagcggctg aacaaattca gaatgtgctt taccagcctg gctggccgca ggtagccatt
    13321 cacgagcata gctttgtcgt tcgcctggct ggtagttttg cggatcaaac ggcatccggg
    13381 tatggaggca agtaagatgc gcttttaccc ttgcctcgct ttcatcacag ttgagcgctt
    13441 catcgtccgg tagataatga ctttgcccga tgtagtcgat cagttcctgc accagtaccg
    13501 acgggaaacg ttcactgtta tcctgaatgg aacgcccgat atagctgata tagagttttt
    13561 gctgcgcgga aattaacgct tccaggaaca ggtagcggtc gtcatcgcga cggctacggt
    13621 cgccacgctt cggtttctgg ctcatcaggt caaagcccaa tggcgcaagc tgacgtggat
    13681 aaacgccgtc gttcattccc agcaggcaaa ccactttgaa cggaattgaa cgcattggca
    13741 tcagagtaca aatgttaacc ggtccggcga gaaaacgctg gctgatacgt tcttgatcca
    13801 gacgctgcgc cagttcatca cgcaatagtg acagcggcac cgcgtcgcca tactgcgcac
    13861 ctaaaccttc ggcgataatc gcctgccatt gttgttcgat cagcgtcatc gccgcttcgg
    13921 tttccgcatc cggcaggaag aaggcgttga gcatatcgcg acaaaccggc aaccactctt
    13981 ctagcggacg ctcctgtgcc agcccgcgac gccagatgtt tagctgcatt agcagtgaag
    14041 ccagatgccc caccagttct gcaattaagc cgctcgattc atcataaggt agaaccgatt
    14101 gccactcgcc ctgcgcgctc tccatcgcgt agcccagcaa catacgcgtc aggccaaatc
    14161 gccaggtgtg ttgtccggtg gcggggagtt ccagctcgcg aacgttgtcg tcatctatcc
    14221 cccaacgaat gccggattcg ttgacccact ggcgtaaata acgcagccct tcttcggtga
    14281 tgtcaaaccg cgccgccagc accggcacat ccagtaatgc cagcacgtct tccgacacaa
    14341 agcggctgtc tggcaacgat aacaggctga taaacgcttc cagtaccgga tgcgactgcc
    14401 gcgcccgacg gtcggaaatg gcgtaaggca ggtaacgatc cgcaggtgcg ctaccaaaca
    14461 cagcctgaat aaacggactg tagctgtcga tatcagccac catcacgatg atgtcgcgcg
    14521 gagtaagtgt cgggtcttct tccagcatcg ccagcaggcg atcgtgcaaa acttcaactt
    14581 cacgctgcgg gctatggcaa acgtggaagg tgatactgct atccagtgga tcaagcgggc
    14641 gtttgttatc gctacgggaa aactcttcga tgttcacacc agcaacggca cggttttcca
    14701 gttccagaat gtcagactga atgttatgca gcaggttatc tggcgtgaca tcgacaaagg
    14761 cgtccagctc ctggctgctc tccaggtcag aaaggagata aatgtagtcg cgcccaagct
    14821 taccccatga agccagcagc gggttgccga catcctgttc accatcgctg ttaaagagct
    14881 gcccggcatt ttcgctatca cgaaatagcg gcaattcgcg atcttcaaca ctgtgtcgac
    14941 gctgacgggt cagcagtttc gccagataag caggatcttt aatatcgccc cagtaataac
    15001 ggcaggggtt ggtaaacagg agatggattt caatatgttt acccagcgcc tggagcgcct
    15061 ggagataaac aggcggtaac gcggaaatac cgcatataaa gacgcgcgaa ggtaaccccg
    15121 gcgggcaggt cgtcgcggac tccagcgttt cgataaagcg ctgatagaga ttggcgcggt
    15181 gccaacgcgg ttgtccgagt tcatgggtat attccaccag cgccttccac aacggcgctt
    15241 gccaggcctg tgcttctccc agcccttcaa ccaaatgtcc tgtttcccac tgtgccagcc
    15301 actccgggcg atagaccaga tactggtcaa acaggtccgc cgcttttgag gaaagctgga
    15361 acagttttcg cttgtcgcta tcgtcagcca gataatgccg caacagggta aagtcttcgc
    15421 gatccagcaa ttgcggcagc agagtcatca gtttccagct catgctctgt ttgttaaagg
    15481 cgctctcttt ggggatctcc ggtaacaccc ggacgaacat atcccagata aagctcgctg
    15541 gcagcggaaa atcaatgttt gccgcaatac caaacttttg cgacagggtc atttgcagcc
    15601 actgcgccat accggtactt tgcaccagaa tcatctctgg ttcgaaagga tcgtccagcc
    15661 gttcgcgttc gacaataaac tccatcaacg cttccagcac gtccagacga ttggaatggt
    15721 agacccttaa catagcggct cctgactact gacgattcgg gcaatgcagg cgggtcatct
    15781 cgccttctct gcccccgggt gaaactagcg taacgctgat gctgacacat cccgcctgcg
    15841 atgtctgcat tcggttgacc tgccagttgg caggtggcga aatcgcgcgc agttgcgttt
    15901 gctgccaacc atgccgccag agctgttggt actggtttct gctggcgaga ctgttcatta
    15961 atgttcgctg gatacccgat aacgcagtga caatcatcac catcagcacc atcgcaacca
    16021 ttacctccgg caggctaaag ccttgttgat tcttcaggga agctgacata acgccctctc
    16081 tttcagcgga caaaaatcgc tccagccgcg tggcgaaaaa acaatgtttc catcaatgac
    16141 ttcgcctgtt cgccacaacg aaacgccttc ataaccggca accaataagg cttcattatc
    16201 tgccagtaaa cgcaaacaaa cccgggcatc ggttccagcg tactgcgagc actgaactgc
    16261 gggctgcgtc tgccaggagt gcattttccc ccacgccagc gccgactgaa cgatggcctg
    16321 gcggcgcaat gactgacttt ccatgctcac gcgagaggca aaactgcgat cctgctgact
    16381 cattccttgc aatagcaagc tacccaaaac cagcagcatc aggaccagag ccagtgacga
    16441 aacacctttt tcgcggttca caggttgaat cctgtcacgc tatagctggc atccaccacg
    16501 gtttgcggtt cagacttact ggcagcacgc atattaaccg tcaacaccgg cgagaagccg
    16561 ctgacatcct gacgtacgac ctgaaaagtg tcgataatga tggcatccgg attagtgact
    16621 ttatcccagc ccttaccttc acaggatgtc gcaccgcgta gcgtttccag cacatgctcc
    16681 ttcagacgaa atccaatctg gtcagactct tttaccggtt cgcgatccca gataccgtta
    16741 ctgttcgcat cccactgcac aatgatacag tcaccctgtc cgacaatttc cagcccttcg
    16801 ccggtacagc tgccatgaca ataacccgcc ctctggagat gcttcgcgac ggtaaatacc
    16861 cgcagccaga tttcatcttc cagcgccagc ttacgggtgc tcgttaaact ttcacgctgt
    16921 aacgcaggca gaaagcgtgc agcccccagc aacaatacgc tactgatcgc catagcaatc
    16981 aacacttcca gcagagaaaa accttgctct tttacaggca tccttctgtt tctccttgct
    17041 gacaaagccg gagtcttccc cacggcgaaa ccaccagcca ccactcgccc gttgagtttt
    17101 tgaagcgaat atgcccggcc catgcggtat tgcgcaggcc aaagaaagca agcgaaggtg
    17161 tcaggtcgct catttcgact tcgggccagc gtggcacaaa gaccaatggt gaactgccat
    17221 gacaggtatt ggccccagca gcggaactca caaggcacca taacgtcccc tccctgataa
    17281 cgctgatact gtggtcgcgg ttatgccagt tggcatcttc acgtaaatag agcaaatagt
    17341 cccgcgcctg gctggcggtt tgccatagcc gttgcgactg ctgccagtat tgccagccat
    17401 agagtccact tgcgcttagc atgaccaaaa tcagcatcgc gaccagcgtt tcaatcagcg
    17461 tataaccacg ttgtgttttc atgccggcag tatggagcga ggagaaaaaa agacgagggc
    17521 cagtttctat ttcttcggcg catcttccgg actatttacg ccgttgcagg acgttgcaaa
    17581 atttcgggaa ggcgtctcga agaatttaac ggagggtaaa aaaaccgacg cacactggcg
    17641 tcggctctgg caggatgttt cgtaattaga tagccaccgg cgctttaatg cccggatgtg
    17701 gatcgtagcc ttcaatctca aagtcttcga aacggtagtc gaagatggat tcgggtttac
    17761 gtttgataat caacttcggc agcggacgcg gttcgcggct taattgcaga tgagtttgat
    17821 ccatatgatt gctgtacagg tgcgtgtcgc cccccgtcca gacaaaatcg cccacttcca
    17881 gatcacactg ctgcgccatc atatgcacca gtaacgcgta gctggcaatg ttaaacggca
    17941 ggccgaggaa aacgtcacag gagcgctgat aaagctggca agagagtttg ccgtctgcca
    18001 catagaactg gaagaacgca tggcacggtg ccagcgccat tttatccagt tcgcctacgt
    18061 tccacgctga aacaataatg cggcgcgaat ccgggtcgtt tttcagctgg ttcagtaccg
    18121 tagtgatctg gtcaatatga cgaccatccg gcgttggcca ggcgcgccac tgtttaccat
    18181 acactggccc gaggtcgccg ttttcatcgg cccattcgtc ccagatggtg acattgtttt
    18241 cgtgtagata agcaatgtta gtgtcgccct gcagaaacca cagcagttca tggatgatgg
    18301 aacgcaggtg gcaacgttta gttgtcacca gcgggaatcc atcctgcagg ttaaaacgca
    18361 tctgatgacc aaaaatggaa agcgttccgg ttccggtacg gtcgtttttc tgtgtgcctt
    18421 cgtcgagcac tttttgcatc agttctaaat actgtttcat ggttcctcag gaaacgtgtt
    18481 gctgtgggct gcgacgatat gcccagacca tcatgatcac acccgcgaca atcatcggga
    18541 tggaaagaat ttgtcccatg ctgatgtact gcacccaggc accagtaaac tgcgcgtcgg
    18601 gctggcggaa aaactcaaca atgatgcgaa acgcgccgta accaatcagg aacaaacctg
    18661 agacagctcc cattgggcgc ggtttacgaa tatacaggtt gaggataata aacagcacta
    18721 caccttccag caacagctcg taaagctgtg atgggtggcg cggcagcaca ccgtaagtat
    18781 cgaaaatgga ttgccactgc gggttggttt gcagcagcaa aatatcttct gtacgggagc
    18841 cagggaacag catggcaaac gggaagttcg ggtcaacgcg gccccacaat tcaccgttaa
    18901 taaagttgcc cagacgcccg gcaccaagac caaacggaat gagtggtgcg ataaaatcag
    18961 agacctggaa gaaggaacgt ttagtacggc gggcgaagat aatcatcacc acgataacgc
    19021 caatcaggcc gccgtggaag gacatgccgc cgtcccagac acggaacaga tacagcggat
    19081 cggccataaa ctgcgggaag ttgtagaaca gaacataacc aatacgtcca ccgaggaaga
    19141 cgccgaggaa gcccgcatag agtaagtttt caacttcatt tttggtccag ccactgcccg
    19201 gacgattcgc ccgtcgtgtt gccagccaca ttgcaaaaat gaaacccacc agatacatca
    19261 ggccgtacca gtgaagcgcc acgggtccta ttgagaaaat gaccggatca aactccggaa
    19321 aatgcagata gctactggtc atctgtcacc acaagttctt gttatttcgc tgaaagagaa
    19381 cagcgattga aatgcgcgcc gcaggtttca ggcgctccaa aggtgcgaat aatagcacaa
    19441 ggggacctgg ctggttgccg gataccgtta aaagatatgt atatgatccg cgctataacc
    19501 ctccgcgaat cagcccgccc atgccacgac gctccataaa ggctgcaacc tgatggcgaa
    19561 cttcggtcgc cagttgcgct tccagactac gctgcgcaag attttctgct tcggcataat
    19621 caatgcgccg cagcaggtat tttacccgcg ctacagaacg tccgttcata gacagatggc
    19681 gatacccaag cccaatgagg attgccacgc acatgggatc gcccgccatt tcaccgcaca
    19741 aacggagatc gattccatgt atttccgctt cccgggcgat catcgccaga gctcgtaaca
    19801 ttgcaggatg aagactgtca taaatgttcg ccacccgggt attgttgcga tcaacggcca
    19861 gaatgtattg agtcagatcg ttggtgccaa cagagatgaa atcgacccgc tttgccagat
    19921 gcggcagcat aaataccatt gacggcactt ccagcatgat gccgatacgt ggtttgggaa
    19981 tttcgtaacc gatcatctcc tcgacttcac gtccggcacg ttcaatcagg cggcgtgctt
    20041 catcaacttc atcgaggctt gtgaccatcg gcaacagaat attcaggttg cccgtagcgg
    20101 cattagcacg cagcatcgcc cgcacctgga tcaagaagat ctccggctga tcgagcgtaa
    20161 tgcgaatccc acgccaaccc aggcatggat tctcttcgct gatcggcatg taaggcagct
    20221 gcttatctgc tccgacatcc agcgtacgca aggtgacggg tttatcattg aacatttgca
    20281 gcatcccctg atactgcgcc acctgttctt cttccgacgg gaaaccactt tgcagcatga
    20341 atgggatttc agtgcgataa agaccgatgc catcaatacg gctgcccagt ttttcttcat
    20401 gttccgggct taaaccagca ttgagcatga cttttatacg ctcaccgctt tttaactgcg
    20461 cgggtaaatt gacgtcatct tccgccagac ggctaagctc aatctcttca ctaattagcc
    20521 gctgatattc ttgcagcagt accggctccg gatcgaccag caattcaccg cgatagccat
    20581 caacgatcag cgtcctacga tgcagcaccg aaggctgaat atccgcgccc atcacggtag
    20641 ggatccccag cgcacgtacc atgatcgcag catgggagtt ggctgcgcca tctcgcacga
    20701 caacaccgac taagcgatcc tggggcagct cagcaagagt tgtcgctgac agttcatctg
    20761 ccaccagaat gaaacgttcc ggccaggcgt tcggcccttg attagcgtca tcaagatgaa
    20821 acagcaatcg ctgacccagc gcacgtaaat cgccagcccg ctctttgaga tagttatcgc
    20881 ttagcgcggc aaactgttcg gcaaattttt caatgaccgt ttttaccgcc cactctgcca
    20941 ccgagccttt atcaacctcg gcaaacaatt cgcgacgcag ccgggtgtcc gaaagtaggt
    21001 gcgagtaaag atcgaaaata gccgccgttt ctttttgtgc tccggcggca aagcgtttgc
    21061 tgtagcggcg aaactcgttt gccgcttctt ccagcgcccc ggtcagtcgt tcgcgttcca
    21121 gagccggatc cagcgttgat gcctgataca cctgttccat taaaggtaac gtggcatcct
    21181 gccagccttc ggcaatcgcc acaccaggtg ctgccggtaa tgcgcggatt cgcgtctggc
    21241 gatattgccc aaacagggca gtcaactgcg actgggaaag aatagctgcc atctgggtgg
    21301 caagcgtcac caggaaggat tcttcacttt cgtcatactg gcgcaactct cgttgctgta
    21361 ccaccagtac accaagcaac tggcgacgtt gaataattgg tacgcctaaa aacgcgcgga
    21421 aacgttcttc ttttacggag gggatgtatt tgaagctggg gtgcttttgc gcatctgcaa
    21481 ggtttatcgg ttccgccagc ctgccaacca ggccgacgat cccttcatca aacgcgagcg
    21541 ttacagtgcg accgcgtggt tttttcagcc cccgggtcgc catcaggtag taacaacgtc
    21601 gatcatgatc ggccaggtag accgaacaga cctcggtatc catcgcaaga cagatgtcgg
    21661 taaccagaat atttaacgcc tcattcaggc gtggtgcgct ggctaccttt tcgactattt
    21721 cgcgcaggcg agtgagcata attggcgtga cttaacctct tttacgtcga taagcagatg
    21781 cgttttgtgg ttttggcgta ttttcctgca gtgacatcac cacactcgcg aactctttca
    21841 ttaccctacg gtagacatca cgtttaaatg acaccacctg tctgaccgga taccagtaac
    21901 ttacccatcg ccagccgtca aactctggtg tactgctggt ttgcatattg atttctgcat
    21961 cgccgcttac cagctgcaag agaaaccatt tttgtttttg gccgatacaa accggcttcg
    22021 tgtcccaacg caccaaacgt ttcggtaatt tgtagcgcaa ccagttacgc gttgaagcaa
    22081 ggattcgaac gtctttgcgg cttaatccta cttcttcaaa caattcacgg tacatcgcct
    22141 gctctgcgga ttctccgggg ttgattccgc cttgcggaaa ttgccaggag tgctgaccaa
    22201 atcgccgggc ccacattacc tgcccctggc gattacaaat cacgatacct acgtttgggc
    22261 ggtagccatc gtcatcaatc accggactac ctcaaaataa agctttatat acgaatgatt
    22321 gtttcatact ccaggaagac ggtaaaccac tctctgcagg gcattacaca ctaataacaa
    22381 ttgaataact cacagttatg tgcagagtta taaacagagg aaggggtgga tagccgtttt
    22441 tattcacctt ttctgtggat agagttgtga agaactacgg aattactacg ggaaaacccg
    22501 gagcattctg aataagcccg atatagacat tttaaaatat tcattattag tcaatgcatt
    22561 acatgtaatt tgagtaatga aaaagagtaa ttcgtgaccc agctcacacc tctcatttac
    22621 gggttggcga aagatcaacc aatgccgtat ttatccacag aatgtgccac taagttaagc
    22681 actgaaccac taaaaactgg agtttcgtcg cacgtcaagg ctgtaaatgg aaacagtagt
    22741 ggaggttttt cacagttatc ccagctttct gtggataaca tggtgtaaga tcctgtttat
    22801 tttcagtgac cagatttgga aaacctattg cagtgttgcg caactcgatt accggcagcc
    22861 taaaaagcaa tataaatcag agaattgaac aacacatatg gaaaaagtta cactgcgaat
    22921 attcggcaca taattgctgt ttgtttttta accaaggtat catgacatgt cccaacctcg
    22981 cccactgctc tctcctcccg aaactgaaga acagttgtta gcgcaagcac agcaactttc
    23041 tggttataca ttgggagaac tggcggcact tgccgggctg gttacgccag agaatttaaa
    23101 acgcgataag ggctggattg gcgtgttact ggagatctgg ctaggtgcca gcgcagggag
    23161 taaacctgag caagattttg ctgctctggg cgtggaactt aaaactatcc ctgtggatag
    23221 tcttggtcgt ccgctggaaa caacattcgt ttgtgttgcc ccgttaacgg gtaatagcgg
    23281 ggtgacctgg gaaaccagcc atgtgcgcca caagctcaaa cgcgtactgt ggataccggt
    23341 tgaaggcgag cgcagcatcc cgctggcgca gcgtcgcgta ggatcaccgt tgctgtggag
    23401 cccgaatgaa gaggaagacc gacagctacg cgaagactgg gaagaattaa tggatatgat
    23461 tgttctcggt caggttgagc ggatcaccgc tcgtcacggg gaatatttac agataagacc
    23521 gaaagcagcg aatgcgaaag cgcttaccga agccattggt gcccggggcg aacggattct
    23581 gacgctgcca cgcggctttt atttgaagaa gaatttcacc agtgcactac tggcccgtca
    23641 ttttctgatc cagtagccat cgctttgacc tgccgctttc cgggcatata attaccgctt
    23701 catttttttg gcagggcttt ttagatgtta tttgcatgga taaccgatcc taacgcctgg
    23761 cttgcgctcg gtacgctgac gctgctggag atcgttcttg ggatcgacaa tattattttc
    23821 ctttctctgg tggtggcaaa gcttcccaca gcacaacgtg ctcatgcgcg ccgtctgggg
    23881 ttggcgggag ccatggttat gcgtctggcg ctgctggcat caatcgcctg ggttacgcgc
    23941 ctgacaaatc cgctttttac aatattcagt caggaaattt ctgcccgtga tttgattctg
    24001 cttctgggtg gcttgttcct tatctggaaa gccagcaagg aaatccacga atccattgaa
    24061 ggtgaagaag aagggctgaa aacacgcgtt tcatcattcc tcggcgctat cgtgcagatt
    24121 atgctgctgg atattatctt tagcctcgac tcggtgatta cggctgtggg tctgtcagat
    24181 cacctgttta ttatgatggc agccgtggtg attgccgtag gcgtgatgat gttcgccgcg
    24241 cgctcgattg gtgattttgt cgaacgccat ccttcggtaa aaatgctggc gctctctttc
    24301 ctgattctgg tgggctttac cctgattctg gaaagtttcg acatccacgt accgaaaggt
    24361 tacatctact tcgcgatgtt cttctctatt gcggttgaaa gcctcaactt gattcgcaac
    24421 aaaaagaatc cgctctgata ctccgtacgc tctcctgcgg gagggcgtac tcactccttt
    24481 taccctcaca gtttaagatt ttcctgcttt caagatatat aacgtcggtt tataaacaga
    24541 ctattatcat aggtaggcca aatgaggata accggatgaa aaaatgggca gtaataattt
    24601 ctgcagtcgg actggcgttt gctgtttccg ggtgttccag tgattacgtc atggcgacca
    24661 aagatggccg tatgattttg accgatggaa aacctgaaat tgatgatgat accgggctgg
    24721 tgagttatca cgatcagcaa ggtaacgcga tgcaaattaa ccgtgatgat gtttcgcaaa
    24781 ttattgaacg ttaacaaata aggtcagcat ccggctggcc ttaagatttt tctctttccc
    24841 ttttcccttc cctctgccat ttttatattc cttatgtcgt gattataaaa aggaaacggc
    24901 tatgcaatat caccgtatac cccacagttc gctggaagtc agcacgctgg ggcttggcac
    24961 gatgacgttt ggtgaacaga acagcgaagc cgacgcccac gcacaactcg actatgccgt
    25021 cgctcagggc attaacctta tcgacgttgc cgaaatgtac ccagtacctc cgcgccccga
    25081 aacgcaaggg ttaaccgaaa cctacgtcgg caactggctg gcgaaacatg gcagccgcga
    25141 aaagttaatt atcgcctcca aagtgagcgg accgtcgcgc aataatgaca agggcatccg
    25201 cccggatcag gcgctggatc ggaagaatat ccgcgaagcg ctgcatgaca gcctcaagcg
    25261 cctacagact gattacctcg atctttatca ggtgcactgg ccgcagcgcc cgaccaactg
    25321 cttcggcaaa ctcggttata gctggacgga ttctgcgcct gcggtttcgc tgctggatac
    25381 gctggacgca ctggcagagt accaacgcgc gggaaaaatt cgttatatcg gcgtgtcgaa
    25441 cgaaactgca tttggcgtaa tgcgctacct gcatctggcg gacaaacacg atctgccgcg
    25501 tattgtcacc attcagaacc cttacagtct gttaaaccgc agttttgaag taggtctggc
    25561 agaagtcagc cagtatgaag gggtcgaact gctggcctat tcgtgcctgg gtttcggcac
    25621 gctgaccggg aaatatctca atggtgcaaa acccgctggc gcacgtaata cgctctttag
    25681 tcggttcacc cgctatagcg gtgagcaaac gcaaaaagcc gtcgcggcgt atgttgatat
    25741 cgccagacgt catggcctgg accctgctca gatggcgctc gcgtttgtac gccgtcaacc
    25801 gtttgttgcc agcactctgc tgggcgcaac cacgatggat cagctgaaaa ctaacatcga
    25861 aagtttgcat ctggagttaa gcgaagacgt attagctgaa attgaagcgg tgcatcaggt
    25921 ttatacttat ccggcaccat aagattaacg ccggttaaaa ccggcgttaa atattaatga
    25981 cggcgctgcc agatccacag cgccgttatt gccagcgcaa acagcgcacc gaagccaatg
    26041 ccaatgggca cgaccgggat gcctatcatt accgccagcg agtaaatgcc cagcatcaac
    26101 aacatggcgc tgttttcgcc aaggttttgt actgcaatcg cattccccgc cccgacgctt
    26161 tttttacccc gctcctgtag caacgcattg agcggaacga caaaaaagcc ccccatcacg
    26221 ccaatcagca tcaacaaggc ataggctggc agcagctcgt gttgcaggga aaaaatcagt
    26281 accaccacgc caatcaaaat cccggctggc atacagcgtg acacggtttc cagcgtaact
    26341 aacttcgctg ccgcacctgc gccaaccacg ataccaatcg ctaccatcgc gttgagatag
    26401 gtgggcgtag cgttatcggt aatgcccagc gccaccggta cccacagcac caacaggaaa
    26461 cgcagcgtga cacccgctcc ccagaataaa ctggtgccca ccagcgaaaa acgcgtttca
    26521 ccattgcgcc atagcgaggt gcaggcattc aggaaactgc gggtcatgtt gatgagattc
    26581 caggactgcc ccggacgcgc cgccgccagt ttgggaatgt agatattggc aacgaccgca
    26641 ccaccgtagg ccagtgcgca tgcggccagg gcgacgagga catgccagtc agccagcaca
    26701 ccaccggcta cggaaccgag caaaatcgcc gctatggtag aagcttccat taaaccgtta
    26761 gctttcacta acttactacc cgtggttaat tcgccgagaa taccgtattt cgccggtgaa
    26821 taggctgcag caccaacacc caccagcgta tagccgagaa acggattgat accaaagcag
    26881 atactggctg cgcccagcag cttcaggccg ttggcaaaca tcatcacccg gcctttggcg
    26941 aagctatccg ccacctgccc gacaaacggc gcaaaaagaa tgtaagcacc tacaaacacc
    27001 atttgcagga tgggctggct ccactccgga tagaactgcg ctttcagtaa cgccagagtg
    27061 gcaaacagta gggcattatc gccaaacgca gagagaaact gcgccacgat aaccgctttc
    27121 atccccttcg accacaacga agtgttagtg tgcactgact cactcatcgt gttgttccgc
    27181 ttcgtctacc cagcttttca acgtgacaaa gtcaggtttg ccgctgccaa gtaatggcat
    27241 ctgtttcaga tagcgaatat cgcgcggtac agcaagctcc ggcacgccgt gctcgcgggc
    27301 atactgttgc aacttatcgc gcgtcagttc gttatctgtg gtgaaaagca ccagtgcctc
    27361 gcctttgctg gcatcgctct taatcgcagt ggcatggact ttatctggcg aaacaccaag
    27421 tgccagttgt tccaccattt ccagcgacac catttcgcct gcaattttgg caaagcgttt
    27481 tgcgcggccc tgaatctgca caaagccctg ctcgtcaaaa cgcacaatat cgccagtgtc
    27541 ataccagccg cgctccattt cgccgcgaac attctcggcg gtgggcactt ccagtacacc
    27601 tggcttctcc acccgcagat agccgttcat tatgttcggc cctttcagtt gcaggcgtcc
    27661 gccctcttcg ataccaggaa ccgacaacag gcgcgcatcc attcctggta gaatacgccc
    27721 taccgtaccg ggtttcgccg ccatcggtac gttgatagaa acgacaggcg cgcactcggt
    27781 tacaccatag ccttcaagga tgcgcaggcc aaatttatcc tgccaaagct gtttggtact
    27841 ttcttgtagt ttttctgcgc ctgccaccac atagcgtaga cgatagaagt catacgggtt
    27901 ggcgaaacgc gcgtagtgac cgaggaaagt cgaggtaccg aacaacacgg tgcaactgcg
    27961 gtcatacacc aactccggca caatgcggta atgcagcggg cttggataaa ggaacacttc
    28021 tgcacctgta agcagtggcg taaacaggcc taccgtcagc ccaaaggagt gaaacagcgg
    28081 taatgccgac ataaagcgat cgttggtggt gaagtcggca atcgttttaa tctgctcgac
    28141 gttcgccaga atgcttttat ggctatggac gacgcctttt ggatggcctt cagaaccgga
    28201 ggtaaagagg atcagcgcct cttcttccgg ctgctgtttt acctgcgcca ggcgcggcat
    28261 cagcaaatga gcgaagatcc atactttgtc ggcagtggtg acatctgctt ttaaatcttc
    28321 cagatagacc cagcgcacct gagtaagttg ctccggcaga tgccagagtt tgcctttatc
    28381 gagaaactgg cgggaagtga agatggtttt gatttcagcc gccgtaatag cactggtcag
    28441 cccttttacc ccagcagtgt agttcatcat tgcgggaatg cggcgacgcg cgatggcccc
    28501 aaaaatcact gccgcactga tgcctgcatt gggcagcatt aagccgatgc gttcgccttc
    28561 aacactgtat ttttcaagga tgcgtccaac aaacagcgtt ttcgtaagca atttgcgata
    28621 ggagtctggg gtaaagttga cgtcttcgac acatttcttc ccggctccga agcggtacat
    28681 tgcactcagt aaagattcgt acagagtttc acgcgggcgc accgccatac gcgcttccat
    28741 cattatttga tgcagcattt cgccagcgat tttgcgacgg tcacgggcac gcggcgcatc
    28801 cggcatcgcc acctgcgttg gtggcaaaat atgcagagta atttgcggga acaagcgacg
    28861 tttaaccaga cctttcaggc ggctgaagtg cgtaagttcc gccccttcaa tacgcacagg
    28921 aataaccgtt gcaccagact tcgccgcgac aaaacccgcg ccatcgtaga ttttcatcag
    28981 cgagcctgta gtggtgatgc gtccttcagg gaaaatcacc actggtcggc cctgttccac
    29041 cagacgtacc agatgtttaa tagccatagg ttgcgtcggg tcgagaggaa caaagtcgat
    29101 aaatgatttc agccaacgca tataccactg ttggcttatt gaggtgtaaa cggcaaacac
    29161 tggacgcaca ggtaaaaaca gtccaagcaa aatgccatca ataaaagaga cgtgattagg
    29221 cgtaattaga acgcgctcgc ccttcagtgc ctgggtgtca cccgtaacgc gaacgcgata
    29281 caaaacacgg cacaaatttc gaaaaaagct aaaaagcatt ccaactccct ttgctctgat
    29341 tcagtaaaag cgaatggagg gagattacac gagataaaga acgcgagcga cagtaaatta
    29401 ggtgcgaaaa aaaacctgcg catccgcgca ggttggtgca agagacaggg tacgaagagc
    29461 gtaccgaata atctcaccaa tcaatacctc tgggatcttg attgtggtct gcacgacgac
    29521 tcttcgccag cgagaaaacg caaaggaatg aagggaaatg caacgaggtg tgtaaattgt
    29581 cggttactgt tacagattga tgaccggcaa aaaaaaacct gcgcatctgc gcaggctggt
    29641 gtaattcatg tgctcaaccc gaagttgact tcacctatca atacctctgg gatcaccact
    29701 ttagcaacct gaagccaaac gccaccagcg gtcaatcgca acagcgtttc gcaaagtgta
    29761 accaaaggtt tgcaatctct ttttttgtct tacttatttg cgtttggctc gcattcccac
    29821 gatgaaaaca cgccacccct tgaaccaacg ggcgttttcc gtaacactga aagaatgtaa
    29881 gcgtttacct actaaggtat tttcatggcg accataaagg atgtagcccg actggcaggc
    29941 gtttcagtcg ccaccgtttc ccgcgtcatt aataattcac ctaaagccag tgaagcttcc
    30001 cggctggctg tgcatagtgc aatggagtct cttagctatc acccgaacgc caacgcccgt
    30061 gcgctggcgc agcagaccac tgaaacggtc ggtctgatcg ttggtgatgt ttccgatccg
    30121 tttttcggtg caatggtgaa agcggtcgaa caggtggctt atcacaccgg taatttctta
    30181 ttgattggca acggttacca caacgaacaa aaagagcgtc aggctattga gcaactgatc
    30241 cgccatcgct gtgctgcgtt ggtcgtccat gccaaaatga tcccggatgc cgatttagcc
    30301 tcattaatga aacaaatgcc cggtatggtg ctgatcaacc gtatcctgcc tggctttgaa
    30361 aaccgttgta ttgctcttga cgatcgttac ggtgcctggc tggcaacgcg tcatttaatt
    30421 cagcaaggtc atacccgcat tggttatctg tgctctaacc actctatttc tgacgccgaa
    30481 gatcgtctgc aagggtatta cgatgccctt gctgaaagtg gtattgcggc caatgaccgg
    30541 ctggtgacat ttggcgaacc agacgaaagc ggcggcgaac aggcaatgac cgagcttttg
    30601 ggacgaggaa gaaatttcac tgcggtagcc tgttataacg attcaatggc ggcgggtgcg
    30661 atgggcgttc tcaatgataa tggtattgat gtaccgggtg agatttcgtt aattggcttt
    30721 gatgatgtgc tggtgtcacg ctatgtgcgt ccgcgcctga ccaccgtgcg ttacccaatc
    30781 gtgacgatgg cgacccaggc tgccgaactg gctttggcgc tggcggataa tcgccctctc
    30841 ccggaaatca ctaatgtctt tagtccgacg ctggtacgtc gtcattcagt gtcaactccg
    30901 tcgctggagg caagtcatca tgcaaccagc gactaaccgc agttaaagca attccagcgc
    30961 cagtaattct tcgatggtct ggcgacggcg aatcaaccgc gcctgaccat tatcaaacag
    31021 aacttctggt aacagcggac ggctattgta gttggatgac attgatgcgc catatgcccc
    31081 tgtatcatgc agtaccagat aatcacccgc cttcacttcc ggcaaggcgc gggtttcaac
    31141 atttccccct tcctgctggg taaagacatc gcccgattca cataatggcc cggcgacgac
    31201 ggtttccacc gttggcgcgt gttccagaga acgaccatca gctgccaggg cactgatatg
    31261 gtggtaacta ccgtacattg ccgggcgcat cagatcgttg aacccggcat caaccagcac
    31321 aaagtggcgg ctacccattt gtttgacgct ccgcacctga gtaattaaca cgccagactg
    31381 cgctaccaga aagcgacccg gttcaatttc cagtttcaca gggtggccca aatggcgagc
    31441 gatttgctca cgcgcggcat tccacagacc ataataatgt tcggtatcaa ccgcctcttc
    31501 accctgttga taaggaatag aaagcccacc gcccgcagaa atagcctgta aatcctgacc
    31561 gaattcgatg acctgacgta ccatagcacc acacacctgt tccagatggg cataatcaac
    31621 gccagaacca atgtgcatgt gaatgccgac cagctgcaaa tgatgacgtt gtatcacgtc
    31681 cagtgcggcg ggcagatcgg tgtaccagat accgtgcttg ctgttttcgc caccggtatt
    31741 ggttttttgg ctatgtccgt gaccaaaccc cgggttaacg cgcagccata cccgatgccc
    31801 tggcgaaacc tggcccagtt ggtcgagcat atcaacagaa cccgcattca ccggaatttg
    31861 caattcactg acgcgttcaa gcgtcgcctg atcgataaca tctgccgtaa aaacaatatc
    31921 atcggggtgc gtttgcggat tgtaacccgc cgccaacgca cgctctattt cgcctaacga
    31981 gacggaatcc actttcacgc cctgctcacg cattaatcgc aaaatatgaa tattggaaca
    32041 ggctttctgt gcaaagcgca ccacatcaaa ctgtttcagc gctgcaatct gccgacgaat
    32101 aatttgcgca tcgtagaccc acaccgggca gccaaattca gcgggcaaac gcagcagatt
    32161 ttcggcggtg agatcggtat cggtgctgaa cagtgaatgt ggcataacaa actccagatg
    32221 agtgcttttt tatgattacg ccacatcata aaaagaataa aaaatatcga tttatgtcga
    32281 gtctatgcaa aaatgatatg gattaccgga ttgcgagaga gcgctaatgg ccgccgttaa
    32341 cttacgtcat attgaaattt ttcatgcggt aatgaccgcc ggaagcctga ctgaggcagc
    32401 acacctgcta cacacctcac agccaaccgt cagccgcgaa ctggcgcgct ttgagaaggt
    32461 gatcgggcta aaattgtttg agcgcgtacg tggacgatta catcctaccg tgcaaggact
    32521 gcgtctgttt gaagaagtgc aacgatcctg gtacggactg gatcgcattg tcagcgccgc
    32581 agaaagtctg cgcgagtttc gccagggaga actgtctatt gcctgcctgc cggtcttttc
    32641 gcaatctttt ttaccgcagc tcctgcaacc ctttctggca cgttatcccg atgtcagctt
    32701 aaatatcgtg ccccaggaat caccgctact tgaagagtgg ctctcggccc agcgtcatga
    32761 tttaggactc actgagacac tccatacgcc tgcgggaaca gaacgtaccg aattactctc
    32821 tttagatgaa gtgtgtgtgt tacctccggg tcatccgctg gcggtaaaaa aggtattaac
    32881 gccggatgat tttcagggtg agaactacat cagcctttcc cgtactgaca gctatcgcca
    32941 gttgctggat cagctattta ctgaacatca ggttaaacga cgcatgatcg tagaaaccca
    33001 cagcgccgcg tcagtctgcg caatggtacg ggcgggggta ggtgtttcgg tggttaaccc
    33061 gctcaccgca ctggattatg cggcaagcgg tttagtggta cggcggttca gtattgcggt
    33121 tccgttcacc gtcagcctga tccgccccct gcaccgcccg tcatcagcgc tggttcaggc
    33181 gtttagtgag catttacaag cggggttacc gaaactggtc acttctcttg acgcgatttt
    33241 gtcgtcagct acgacagcat aaaagcgaca gcatcctcgg catggatcgc cgcagtatca
    33301 aacacaggca aaacactgcg ctcttctgga accaataaac caatttctgt gcagccaaaa
    33361 atgacaccct gcgccccctg ttctgcaagg cgagcaatca cttgcgcata ataagcgcgt
    33421 gacgcttcgg taaattgccc cagacacagt tcttcaaaaa taatctgatt aattttcgcc
    33481 cgttcatccg cttcaggaat aagacaattg atggaaaatt gttccgtcag ccgcccgcga
    33541 taaaaatcct gttccatggt gtaacgcgta cccagcagcg ccacacgagt cattccggcc
    33601 ccggtaattg cacgtccggt ggcatccgca atgtgtaaga aaggcagagt gcaacgtgac
    33661 tcaatggcat ccgccacttt atgcatcgta ttggtacata acacaatacc ttctgcgccc
    33721 gcccgctgta agccaagcgc cgcctcagcc agaatgtccc cggttttatc ccattccccg
    33781 cgacgctggc actcttctat ttcatgaaaa tcgacgctat gtagcagcac ttgcgcagag
    33841 tgaagcccac caagccgctg tttaatgcct tcatttatca gacgatagta aggaatggtg
    33901 gattcccagc tcattcctcc cagcaaacca attgttttca ttcctctctc ctgatgtgtg
    33961 ttaccgcaac aaggctgaat aaggaacgag acaaacgcct caacggccag gtgcccgatc
    34021 tctattaacg aaaaaagggc cggatgtaca gcacatccgg cccgtgaaat cagacgccga
    34081 tatttctcaa cttctcgcct gccatcagtt tgcgttcgat atgttccagc gtgacatttt
    34141 tggtttccgg aatgagccag aaagtgatgc ccacaaacgc aatgttcagc gcagtgtaga
    34201 gccagaacgt accggcagcg ccaatgctat caagcagtgt caggaaggtc gcgccgataa
    34261 tcatattcga cacccagttt gtcgtcgtcg aacaggtaat accgaaatcg cggcatttca
    34321 gcggctgaat ttcagagcac aggatccaca ccactggcgc ggcgctcatc gcataaccgg
    34381 caatacacat catcgtcatg ccaacagaga gccaggacaa gccactggaa gccgtaccgt
    34441 tatcaaactg catcaggcaa tagcccagca ccagagtgcc taacgccatc acgctgaaac
    34501 caattttcag agccggttta cgacctgctt tatctaccgt aaagaccgca atgaaggtcg
    34561 cgaacataaa ggtcagtccc acgaccagag tcgcaatcat ctgttgttct gtggtcgtaa
    34621 agcccgccat tttgaagata cgcggcgcat agtacatgat gatgttcata ccggtaaact
    34681 gctgcatcgc ctgcaacaac ataccgagaa acacagcacg acggacgtta cggttgatct
    34741 taaacagtgc ccaaccgcct tgttttaact tcaggctttc acgaatttcg ttgagttctt
    34801 ctcgcgcttt ttccgacgta tcgcgcagca tacgcaacac ttcttccgcc tcaatatgac
    34861 gccccttttc cgccagccag cgcgggctat ttggcaggaa gaccaccaga ataatcagca
    34921 ggactgctgg taaagcaaga acccccaaca ttgcgcgcca gttaccgcta taactgaacg
    34981 ctgtatcgga taaaaacgcc agcacgatgc cgagtgtgac catcaactgg tacatactga
    35041 tcatcttacc gcgaacgttt tcacttgcca tttcagaaag atacagagga gcggtgtaag
    35101 acgcgatccc gacagcaatg cccagcacca cacgagcggc gattaacatc tctacgctgg
    35161 tcgcaaaagc ggaccctata gaaccgagta caaacaggat ggcccccgcc atcaggctgt
    35221 atttacgacc caggcggaac gacagccaac cattaaacag cgcaccaatt gctgcgccga
    35281 gcatcatgct actaaccacc cattcctgca aacgactggt cagcacaaag tgatcggtaa
    35341 tgaacggcaa cgctccggcg attacgccga tatcaagacc aaataacaat cctgcgaccg
    35401 cagcagctac cgaaacaaac atattcatac gccgcgtatc ccgcaaagaa cgtggcgtta
    35461 aagcagattc cgtattgata gtaaccattt tttcctgcca gcagagagta agacatagtg
    35521 aaaaaatacg tgaacaactc acgcaggtgt caggtcggaa acagcataaa tatggattaa
    35581 attgctgcg
//
LOCUS       DAZGJW010000034        35557 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZGJW010000034 DAZGJW010000000
VERSION     DAZGJW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.034"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..470)
                     /gene="sfmF"
                     /locus_tag="ACHKIK_001721"
     CDS             complement(<1..470)
                     /gene="sfmF"
                     /locus_tag="ACHKIK_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO9082913.1"
                     /translation="MRRVLFSCFCGLLWSSIGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRP"
     gene            complement(481..1458)
                     /gene="fimH"
                     /locus_tag="ACHKIK_001722"
     CDS             complement(481..1458)
                     /gene="fimH"
                     /locus_tag="ACHKIK_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO9082914.1"
                     /translation="MACLCLANISWATVCANSTGVAEDEHYDLSNIFNSTNNQPGQIV
                     VLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLHDYLLGAMSLVD
                     SVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMVEIPRQVMFTV
                     YVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASLFSAAGPGNRP
                     AGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIVADQNDTPITP
                     NDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDYQ"
     gene            complement(1500..4109)
                     /gene="sfmD"
                     /locus_tag="ACHKIK_001723"
     CDS             complement(1500..4109)
                     /gene="sfmD"
                     /locus_tag="ACHKIK_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO9082915.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERISLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGIRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGTVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGRKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(4140..4832)
                     /gene="fimC"
                     /locus_tag="ACHKIK_001724"
     CDS             complement(4140..4832)
                     /gene="fimC"
                     /locus_tag="ACHKIK_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO9082916.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRLIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(5052..5594)
                     /gene="fimA"
                     /locus_tag="ACHKIK_001725"
     CDS             complement(5052..5594)
                     /gene="fimA"
                     /locus_tag="ACHKIK_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO9082917.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            6075..6941
                     /gene="folD"
                     /locus_tag="ACHKIK_001726"
     CDS             6075..6941
                     /gene="folD"
                     /locus_tag="ACHKIK_001726"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO9082918.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            6943..7155
                     /gene="ybcJ"
                     /locus_tag="ACHKIK_001727"
     CDS             6943..7155
                     /gene="ybcJ"
                     /locus_tag="ACHKIK_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO9082919.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVEVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            7263..7784
                     /gene="ybcI"
                     /locus_tag="ACHKIK_001728"
     CDS             7263..7784
                     /gene="ybcI"
                     /locus_tag="ACHKIK_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO9082920.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHREFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(7820..9205)
                     /gene="cysS"
                     /locus_tag="ACHKIK_001729"
     CDS             complement(7820..9205)
                     /gene="cysS"
                     /locus_tag="ACHKIK_001729"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO9082921.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYIALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            9379..9873
                     /gene="ppiB"
                     /locus_tag="ACHKIK_001730"
     CDS             9379..9873
                     /gene="ppiB"
                     /locus_tag="ACHKIK_001730"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO9082922.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            9876..10598
                     /gene="lpxH"
                     /locus_tag="ACHKIK_001731"
     CDS             9876..10598
                     /gene="lpxH"
                     /locus_tag="ACHKIK_001731"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO9082923.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            10716..11225
                     /gene="purE"
                     /locus_tag="ACHKIK_001732"
     CDS             10716..11225
                     /gene="purE"
                     /locus_tag="ACHKIK_001732"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO9082924.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            11222..12289
                     /gene="purK"
                     /locus_tag="ACHKIK_001733"
     CDS             11222..12289
                     /gene="purK"
                     /locus_tag="ACHKIK_001733"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO9082925.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(12426..13319)
                     /gene="ybcF"
                     /locus_tag="ACHKIK_001734"
     CDS             complement(12426..13319)
                     /gene="ybcF"
                     /locus_tag="ACHKIK_001734"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO9082926.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVDPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQKALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFTKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(13316..14131)
                     /gene="ylbF"
                     /locus_tag="ACHKIK_001735"
     CDS             complement(13316..14131)
                     /gene="ylbF"
                     /locus_tag="ACHKIK_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO9082927.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(14142..15401)
                     /gene="ylbE"
                     /locus_tag="ACHKIK_001736"
     CDS             complement(14142..15401)
                     /gene="ylbE"
                     /locus_tag="ACHKIK_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO9082928.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(15411..17078)
                     /gene="fdrA"
                     /locus_tag="ACHKIK_001737"
     CDS             complement(15411..17078)
                     /gene="fdrA"
                     /locus_tag="ACHKIK_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO9082929.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELTNQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            17394..18443
                     /gene="allD"
                     /locus_tag="ACHKIK_001738"
     CDS             17394..18443
                     /gene="allD"
                     /locus_tag="ACHKIK_001738"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO9082930.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFFSS
                     KLFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            18465..19700
                     /gene="allC"
                     /locus_tag="ACHKIK_001739"
     CDS             18465..19700
                     /gene="allC"
                     /locus_tag="ACHKIK_001739"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO9082931.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            19711..20496
                     /gene="allE"
                     /locus_tag="ACHKIK_001740"
     CDS             19711..20496
                     /gene="allE"
                     /locus_tag="ACHKIK_001740"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO9082932.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(20625..21770)
                     /gene="glxK"
                     /locus_tag="ACHKIK_001741"
     CDS             complement(20625..21770)
                     /gene="glxK"
                     /locus_tag="ACHKIK_001741"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO9082933.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGETVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(21792..23092)
                     /gene="ybbY"
                     /locus_tag="ACHKIK_001742"
                     /pseudo
     CDS             complement(21792..23092)
                     /gene="ybbY"
                     /locus_tag="ACHKIK_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
     gene            complement(23149..24510)
                     /gene="allB"
                     /locus_tag="ACHKIK_001743"
     CDS             complement(23149..24510)
                     /gene="allB"
                     /locus_tag="ACHKIK_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO9082934.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(24570..26024)
                     /gene="ybbW"
                     /locus_tag="ACHKIK_001744"
     CDS             complement(24570..26024)
                     /gene="ybbW"
                     /locus_tag="ACHKIK_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO9082935.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLIFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENGGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIHFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(26193..27071)
                     /gene="glxR"
                     /locus_tag="ACHKIK_001745"
     CDS             complement(26193..27071)
                     /gene="glxR"
                     /locus_tag="ACHKIK_001745"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO9082936.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(27171..27947)
                     /gene="hyi"
                     /locus_tag="ACHKIK_001746"
     CDS             complement(27171..27947)
                     /gene="hyi"
                     /locus_tag="ACHKIK_001746"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO9082937.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYTPQTTTEAGLRWMDPYR"
     gene            complement(27960..29741)
                     /gene="gcl"
                     /locus_tag="ACHKIK_001747"
     CDS             complement(27960..29741)
                     /gene="gcl"
                     /locus_tag="ACHKIK_001747"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO9082938.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(29831..30646)
                     /gene="allR"
                     /locus_tag="ACHKIK_001748"
     CDS             complement(29831..30646)
                     /gene="allR"
                     /locus_tag="ACHKIK_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO9082939.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(30724..31205)
                     /gene="allA"
                     /locus_tag="ACHKIK_001749"
                     /pseudo
     CDS             complement(30724..31205)
                     /gene="allA"
                     /locus_tag="ACHKIK_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
     gene            31435..32361
                     /gene="allS"
                     /locus_tag="ACHKIK_001750"
     CDS             31435..32361
                     /gene="allS"
                     /locus_tag="ACHKIK_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO9082940.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            32430..33524
                     /gene="mnmH"
                     /locus_tag="ACHKIK_001751"
     CDS             32430..33524
                     /gene="mnmH"
                     /locus_tag="ACHKIK_001751"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO9082941.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            33640..34005
                     /gene="ylbG"
                     /locus_tag="ACHKIK_001752"
                     /pseudo
     CDS             33640..34005
                     /gene="ylbG"
                     /locus_tag="ACHKIK_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(34772..35018)
                     /locus_tag="ACHKIK_001753"
                     /pseudo
     CDS             complement(34772..35018)
                     /locus_tag="ACHKIK_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(34999..35557)
                     /locus_tag="ACHKIK_001754"
                     /pseudo
     CDS             complement(34999..35557)
                     /locus_tag="ACHKIK_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT         9020 a         9391 c         8560 g         8586 t
ORIGIN      
        1 ggccgaacac cgctggctaa cgctcgataa ttggcaaaaa agtttagtgt gacattgcca
       61 tttgcatcta cttcctcagt cgggctcgcc tcccccagtg cgagccggga gcgatcgcta
      121 ttacgtaatt cgatggcgac ggtttgtgcc attgcgggat catccagagc cagcaggttg
      181 gtatcggatg ccggcgttcc cgtaaataaa atcgcaactg aacccggagg acatccctcc
      241 agccgcaggc taaaagggac gagtgccgtg gtatcgccag cgttcagtaa ttgtgtcgta
      301 ggccatctgc ctaaatctac cgtcttatca atatccgctg tgtttacggt acaggagaaa
      361 tcaacaacgt taccgtgcaa attgatatta atcgttccta aagggtcagc tgcccatcca
      421 atggaactcc acagtaaccc gcagaaacag ctaaagagta ctcttctcat tctccgtacc
      481 tcattgataa tcgacgcgta aataccccag cgcgctaaac ggcccctcgg tcggtttttg
      541 accggtaata ctgataggcc aggcgcgaag tgtgacattg gctgccgcag ctgcatccag
      601 acggaaagga ataacgctat tgagatcgtt aggcgtaatc ggcgtatcgt tctgatcggc
      661 gacaataaaa cctaaatcct gattgtccga caccatcgcc tgaccagaaa cggcactggc
      721 ttccagacgc attgttaaat aagcctgcgc agcaacattc gtacatttga ccgcaatgct
      781 cttggtttgc ggcatgacgc cagctggtcg attccccggc cctgccgcac taaataacga
      841 tgcgccgata tcaccaaaat caaattcaac aatctgcccg gcatttaatt cgcagttttg
      901 cggcacttca acccggccac caaaactaat ggtataaaca ggtgtaacca acggatcgta
      961 aggcgttgat gtcacataca cggtaaacat cacctgtctg gggatctcca ccatgttaat
     1021 aaagggacga ataaccttta aacgaaatat taaacgagaa tccatcaccc cgaatggaag
     1081 gttttgcgaa acgttaggat ctgttcccat ccgaatataa ttttgcgggg ggaactgaat
     1141 atccatcaca ctatcaacca gactcatcgc acctaataga taatcatgta attgcatata
     1201 tttataatta tcgatagttt cctgaacaat aaagttggtg acataactac ggtatgtata
     1261 attcaccagc gtaccgggtg gacaaattgc tgagacacct acccagccgg atttttccgg
     1321 taaaacaaca atctgccctg gctggttatt ggtgctatta aagatatttg agagatcata
     1381 gtgttcatct tctgctacgc cagtactatt tgcacaaaca gtagcccagg atatgtttgc
     1441 cagacacaaa catgccatcg ccaataataa tctgcagatt atcttattga taatttcctt
     1501 tatgagccag gatgtgtgca aactgccgaa ataacagtga cggcttgctg taagctttgc
     1561 ttcggcaata catagtgcgc aatgcagcgt gagttcgcgc cgtctcccca ctgaaccagt
     1621 aattcaccag acaatggcgc accacttaaa taaacttgcc cgtcatcacc caccatactg
     1681 gttattccgg tactgttttc ccgtaccagt gatccaaacg gcaccggttt tctgccctgg
     1741 gtaacggtaa tgagcgcccg cacgcctatg cgggtatcaa aattggcacg aaccaacgcg
     1801 ccttgcgtcg gcacaacgct gctaatattt ttttcaacat cgatggaatt ccccatcgta
     1861 ttggtatcaa gcgcgatacg gttataccga taaaccgtgg catacggcat caccgcatag
     1921 ccgcgccagt cggttaaaat gccagtttga ttttcaatgc gtacaccgcc tgcgccaggc
     1981 gctttaatca aaacattggt atcccctaaa ggctggctca gcgttatgcc attttcatgc
     2041 ccgaccacac cgccagacag ctgccagtta acgtcatgtt gatcgcggtc gtagttatac
     2101 ccgaccccca gcgtaccgta agcggcctgc cagtttgccg tggcgctgcc cgtataccca
     2161 ttattcgagg tatcaccctg gcttacgtga taactcaggt tgtggccttc cagtaaggtc
     2221 ccacctacac ctgccagcca gctattttgc ccgttgctgt tacggttggc gttaaaggag
     2281 gcataagcgc ggtcgagcgc attttcccgg gtgtagcgac gtttggtcag aacattgaaa
     2341 ggcactgaaa cattaagtcc gacaatacgt tcgttatcgg ggatccctac agattcattc
     2401 cacgaaaatg agagtgaata actgatgcca acccagctgc tggtataccc cacctgatac
     2461 caggtatctg aatccgaagt attccagtat ttttgatggg taccagagat ataaagcgag
     2521 ccaaagtcat taagtgattg tgaaatattt aactgcaaac ggtctttacg gctaaagcgt
     2581 aaattgtggt aattcacgat tatcggttca tcgcgatgct caccgtcgta atcgtaatca
     2641 tattcgtacc cctccattcg acgatacgca acatcatcaa gggtataaaa accttgtgtc
     2701 gaatagcggt aacccattaa ctgaaaattg gtgccgaagg tgttcatcga tttcgcatag
     2761 aggaagcgaa tggaatcccc ctcatgacga ctgtcgtcgg ctaactgact gcgcgcatgg
     2821 gtcacatcca gcgacactgt gccccagttc ccgaggttgc gccccagccc taataaaaag
     2881 gcggtgtaat tggcagataa ttgcgtcccg ccgtaggcag taaattcctg tggtaaaccg
     2941 ccgagtgccg taccctgaaa aaagaaaggc gatgattgct gactattacc gctacgaaaa
     3001 tcgcccgccg tcaggtcaaa tttgaaacgc ccttcgcgtt gtaaaattgg cactgttgaa
     3061 tacggaattg tgtaattctg ctgattacca tcgcgctcgt cgatggtgac gtccagatcg
     3121 ccatttgaag atgtcgggtg caaatctgta atttcaaaag cgccggggga aacatagctt
     3181 tgatagataa taaaaccatt ttgccgaatc gttagctggg ccgccgtacg ggcaatccca
     3241 cgtactgttg gggcaaaccc ttgctggcta tcaggataca tattatcgga agagtaaagc
     3301 cgtataccac gaaatccaac accatcgaaa atatcacttc ctgtattgcc gtctcccatt
     3361 accagttcac ttttcagcgg aataatggcg cgctgtaccc aggtgccaat attattccac
     3421 tgttctgaat gatatccatt tccgcgaaaa tagttccagg aaccattgtt gcgtaaacgc
     3481 cacgggccaa tattaatccc gctgagctca ctaaaaaaat agctatcgtt accattacct
     3541 ctgttaccgg tgaaattata attcaggagt aaagcaggaa taccttcatc ccactcttca
     3601 ggcggaatgt aaccgtgagc gctactcaac aacgctatct gaggaatagt gatgttcagg
     3661 cgcatcgctg caaaatcaaa gttaattgtg gcatcaggta cagctttcag taaattgatg
     3721 catttattgt tttgctgctg ggctaactcg ggaaatgcac tgctattaag gctaattcgt
     3781 tcaaggagta cctggttaaa acagggcatt aacccacctg atttatcacc tgtattttct
     3841 gttgtcgatt caaatacgat atcctgtgaa ccaatgaact catcattacg ccagagatct
     3901 acccgataca cgcccgcagg ttgatgatta cctctctcaa agcgcgataa atcagcaacg
     3961 gatgtgccat tctctaataa aaatgccggg ttgaaataac tttccgcata gcttaaaaag
     4021 gagggtaaaa tggcaagaga tgaataacaa attccggcca gacaccaggt atacctctgc
     4081 ggaatatccg tagtagtggg tattttcata attagctctg atattgtcag atgattcggt
     4141 tagtttaagt tcacttctct gaccggggtt actgaaccat aatcattaac ggtctgaaat
     4201 ttcagctttc cctgcactcc tgagggtaag ggaatttgtg aattaactct gggtgcagcc
     4261 atagcattcc ccaacttttg gctgcccact tgtaagttaa ccagagtgac ataaaaaggt
     4321 gagggattat gaacattgat atagttaccg gatcgcgaaa acttgagtgt gtccggtgct
     4381 tctgcgggta attcttgtaa ttgaattaga cggagaaata atttcatgcg cgataaaatc
     4441 gccagttgca aaacattcct gccgttcaat gcatttttat ctaccgaagg gatcgtctta
     4501 acattcatcc agaacagaga ctcacgatct gctgccagcg gtggaccggt gtaaataata
     4561 cgcaaggtat tttcgctttt gggttcacta acaaacagtg gcggtgtaat gatgaatgac
     4621 ttttctttta caccgctgct gttttcaatc cacgaattga ccagaaagcg ctcattggta
     4681 tggctatttc taatccatac cgcagtctgt ttagcatcag cgggataaat aatacgcgtg
     4741 gcacctaatg cgatccctcc ggcagcatgg gcgctggccg aaatgattaa ataaaatata
     4801 atgagcatca ataactttat tttagtcatc atagttaata cgccttgtgt attcctgacg
     4861 gcaaaaataa gaaatataaa gaaatgattg ctttggtttc ttacaacaca tcattccctt
     4921 ttcatttaat aaagcggcat gatacaaagt gaacatgatt tcgatttaca ttgccaccat
     4981 accgcttcag acaaccggta cattactttg ttataagacg tgatatataa ttcaaaacaa
     5041 cgtggttttg attattcgta tctcataata aaagtcgcgt cagcatttgc ttgccctgct
     5101 gatgcacttg taccggtgcc tttataacgt gcagaaaaat gaagaacgtt ggtcccgggg
     5161 atcaagttct ggttggttga gaagctattc ccatcaggtt tgagaattgc ggatgtatta
     5221 tcaagtattt caataccgac acccgttgct gttgttgtat tggtactgga tgcaatggcc
     5281 aataaattat cattgattgc atcagcctga ccagaaaatg caacggcagc attagcggca
     5341 acaacaggat cgcagtcgtt caactgaatg gtgaatggaa ttaatgcaga ggtattacca
     5401 acagcattga aaatatcggt acgatattga ccgagtgtga caacctggtc tgccgagtca
     5461 gtattcactg aacaggcagc attcaccagt tcgccttcaa agtgaattgt accgccatct
     5521 acaacggcag cataactacc cgtagcggcg aatagtgcgg cagccagcgc agacgaaata
     5581 aatcttaatt tcatatatat tccttcaatc tcatttatcg actccacatc cgtatataac
     5641 cgattacttt atttaagaca ctgatagtag taaaattcct ttttatcctc taagaatgtc
     5701 ttaattgaaa atatgcactc tattctaaaa aatagagagc cccgttagat gaatactttc
     5761 gcgcaaaata tattcaacac aaatatagac ctgaagcggt aaattaccag gctgaaaatt
     5821 ctttttatat tgtcaggtat ttcttaaatt atcttaatcc ttaaacaagg aaataaatca
     5881 gttccagatt tacaacgcca tcatggacga aaaatgaagc tttcaatctc agcgacctca
     5941 gcgacggtgc gcctcacctt cgcaagaggt cgcttcacgc gataaatctg aaacgaaacc
     6001 tgacagcgcg ccccgcttct gacaaaatag gcgcatcccc ttcgacctac gtaacagatg
     6061 gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg cagcaggtgc
     6121 gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg gcaccaggac
     6181 tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca agcaaacgca
     6241 aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa accaccagcg
     6301 aagcggagct gttggagctt atcgatgcgc tgaatgccga caacaccatc gatggcattc
     6361 tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa cgtattcatc
     6421 cggacaaaga cgtggacggt ttccatcctt ataacgtcgg tcgtctgtgc cagcgcgcgc
     6481 cgcgtctgcg tccctgcacc ccgcgcggta tcgtcacgct gcttgagcgt tacaacattg
     6541 ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt cgtccaatga
     6601 gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgtttc actaaaaatc
     6661 tgcgtcacca cgtagaaaat gccgatctgt tgatcgttgc cgtcggcaag ccgggcttta
     6721 ttcctggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc aaccgtctgg
     6781 aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc gcctcataca
     6841 ttacgcctgt tccgggcggc gttggcccga tgacggttgc cacgctgatt gaaaacacgc
     6901 tacaggcgtg cgttgaatat catgatccac aggatgagta acatggcgac attttcttta
     6961 ggtaaacatc cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg gagcgaaagc
     7021 ggcgcgcagg cgaaaatcgc gattgccgaa ggccaggtgg aagtcgacgg tgcggttgaa
     7081 acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg tcacagcgta
     7141 caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc ttttcagact
     7201 tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc gaagcgacca
     7261 tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta gggttgggtt
     7321 cgaaagtcat ccctccgcgt ctgttatttg ccggaatcat cctggcgatg ctgcccgacg
     7381 ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt catcgcgagt
     7441 ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg attggacgcc
     7501 gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg ttgctatcgc
     7561 acagcttgct ggattcggta accactggcg gtaagggcgt tggctggctg tggccgtggt
     7621 cagatgaacg ctttttcgct ccctggcagg tgattaaagt cgcgccgttt gcattgtctc
     7681 gttacaccac gccgtacggg catcaggtga ttatttctga attgatgtgg gtgtggttgc
     7741 cggggatggt gcttatggga atgttgtggt ggcgcaggcg ataaccggat gcgaaaactc
     7801 gcatccggca acagcgcaat tacttacgac gccaggtggt cccttgcggg ccatcttcca
     7861 gcacgatccc catctcgtta agacggtcac gcgccgcatc tgccgccgcc cagtctttcg
     7921 ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact tcgctgtcgt
     7981 ctgcctgcgc accgctttgc aggaacgctt ccggttcttg ctccagcagg cccaatacag
     8041 cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct tctgctttca
     8101 gacggttcac ttcacgcgcc atatcaaaca gcacggaata ggcttccggg gtgttgaaat
     8161 cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca ggcgcaacgg
     8221 ttttatctgt gccgcgcagc gcaatgtaga ggcgctccag cgccgcacgc gcctgcttca
     8281 ggttctcttc gctgtagttc agttggctgc gatagtggcc cgacatcagg aagtaacgca
     8341 cggtttccgc gtcgtagtat ttcagcacat cgcgcacggt aaagaagtta cccagcgatt
     8401 tggacatctt ctcgcggtca accatcacca tccccgagtg catccagtag ttcacatact
     8461 ggccatcatg ggcacaggtg gactgcgcga tttcgttctc gtggtgcggg aacatcaggt
     8521 ctgaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc attgccgaac
     8581 attcaatgtg ccagcctgga cggcccgcgc cccacggaga cggccagctc ggttcgccct
     8641 ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcgtcg accacgtcaa
     8701 cgcgcgcacc tgcctgcagc tggtcgagat cctgacgcga cagcacgcca tatgtcggat
     8761 cggtcgggac gtcgaacatc acgtcgccgt tgtccgccac ataagcgtga cctttggcga
     8821 tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc ggctccatat
     8881 ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc atgcgatcca
     8941 ccagcgccac aaagctttcg ccattttcat tggcgcgttt gatgattttg tcgtcgatat
     9001 cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag cgcgcaacca
     9061 cgtcaaaagc aacaaaggta cgcccgtgac cgatatgaca gagatcgtaa acggtgattc
     9121 cacacacata catgccgact tccccggcgt gaataggctt aaattcctct ttttggcgtg
     9181 tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg ggtaattgag
     9241 ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat gatccgatcg
     9301 tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag caccaatcaa
     9361 acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat tgtcatcaaa
     9421 acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg ccgcgaaggt
     9481 ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca gggcggcggt
     9541 tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga agccaacaac
     9601 ggtctgaaaa atacccgtgg tacgctggca atggcccgta ctcaggctcc gcactctgca
     9661 actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc tggcgaaagc
     9721 ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg aaggcatgga cgtggtagac
     9781 aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc aaaagaagac
     9841 gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt attgcagatc
     9901 ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt ttagcggggg
     9961 aagcccgcaa ggccgacgcg ctgtatattc ttggcgatct gtttgaagca tggattggcg
    10021 acgacgatcc caacccactc catcgccaga tggcggcagc gatcaaagcg gtgtccgatt
    10081 ccggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc aaacgctttg
    10141 cccgtgaaag cggcatgaca ttattgccgg aagaaaaggt gctcgaactt tatggtcgcc
    10201 gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat caggcttttc
    10261 gcgccaaagt ccacaaaccc tggctgcaaa tgctattcct cgccctgccg ttgtttgtgc
    10321 gcaaacgcat tgccgcgcga atgcgagcga acagtaaaga agccaacagc agtaaatcgc
    10381 tggcgatcat ggacgttaac caaaacgcgg tagtcagtgc gatggaaaaa catcaggtgc
    10441 aatggctgat ccacggacat acccatcgcc cggcggtaca tgaacttatc gccaaccagc
    10501 aacctgcttt tcgcgtggta ctcggtgcct ggcatacgga aggttcgatg gtgaaagtca
    10561 cggcggatga cgttgagctg attcattttc cgttttaaaa aacccgcaac tttgctgatt
    10621 tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt gccctctaaa
    10681 gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat aatccggcgc
    10741 gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc gccgccgaaa
    10801 tcttcgaaat cctgaatgtc ccgcaccacg ttgaagtggt ttctgctcac cgcacccccg
    10861 ataagctgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg attattgcgg
    10921 gcgcaggcgg cgcagcgcac ctgcctggta tgattgccgc caaaacgctg gtgccagtgc
    10981 tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac tccatcgtac
    11041 aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc gcggcaaacg
    11101 cggcgttact ggcagcacaa attcttgcga ctcatgataa agaactgcac cagcgtctga
    11161 atgactggcg caaagcccag accgacgaag tgctggaaaa cccggacccg cgaggtgcgg
    11221 catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc gtcaggcagg
    11281 tgaaccgtta ggcattgctg tctggccggt cgggctggac gctgaaccgg cggcggtgcc
    11341 ttttcaacaa agcgtgatta ccgctgagat cgaacgctgg ccggaaaccg cattaacccg
    11401 cgagctggcg cgtcatccgg cctttgtgaa ccgcgatgtg ttcccgatta ttgccgaccg
    11461 tctgactcag aagcagcttt tcgataagct ccacctgccg accgcaccgt ggcagttact
    11521 tgccgatcgc agcgagtggc ctgcggtgtt tgagcgttta ggtgaactgg cgattgttaa
    11581 gcgtcgcact ggtggctatg acggtcgcgg tcaatggcgt ttacgtgcca atgaaaccga
    11641 acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta acttctctgg
    11701 tgaagtgtcg ctggttggcg cacgcggatt tgatggcagc accgtgtttt atccgctgac
    11761 gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc aggccaatgc
    11821 gcagcagcaa gcgcaagccg aagagatgct gtcggcgatt atgcaggagc tgggctatgt
    11881 gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca acgaactggc
    11941 accgcgtgtg cataacagcg gtcactggac acaaaacggt gccagcatca gccagtttga
    12001 gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccggtagtga atagtccgtc
    12061 ggtgatgatc aacctgattg gtagcgatgt gaattatgac tggctgaaac tgccgctggt
    12121 gcatctgcac tggtacgaca aagaagtccg tccggggcgt aaagtggggc atctgaattt
    12181 gaccgacagc gacacatcgc gtctgaccgc gacgctggaa gccttgatcc cgctgctgcc
    12241 gccggagtat gccagcggcg tgatgtgggc gcagagtaag ttcagttaag caggtgctca
    12301 tttgattgcc ggatgcggcg taaacgcctt atccggctta ccgctccggt acccattgta
    12361 ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc cggatgctct
    12421 catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc aattcgcgat
    12481 aacgccccaa tccacgcggg tttaccacgg cttctgacat aaccacttac cgccgttacc
    12541 ttcggcccca tcgaaccatc ggctttggta aatggcgcta actcatccgg tgtggcatgg
    12601 cgaatggcgc gttgctgcgg cgttccccag ttttcataaa ccgcgtcagc atcggtgagg
    12661 atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcggcgag atctttatca
    12721 atcaccgctt cactccctgc cccatcttcc gtcacaggca caccgccgcc gccactgcaa
    12781 atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag aattttacgc
    12841 ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat ctgccagccg
    12901 taagccgctt ccagtgcctt ttgttcttct ggctgataaa ccggaccaat aaatttctct
    12961 ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac cgtcgtgaca
    13021 ggcggcatct gcggctgtgc gctcaaactc tgcgccagca tatagccaat catcccctgg
    13081 ctttccgcca ccagcacatc cagcggatac ggatctacct ctttccacgc cagattctgc
    13141 aatgccagca gccccacctg cggcccattg ccgtgaacaa tcgccagccg ataagaacgg
    13201 gccaggcgcg ccagcgcggg tacagcactg gcgatattgc gatattgatt ttctgccgtc
    13261 agcgcctcac cgcgctggag taaggcgttg cccccaagag ctacaaccag tgttttcatg
    13321 gttttccttg taataattgt tggccaagcc agaaccccag cagcgtatct gcccctgacg
    13381 tatgccccag cgccagcagc gaatcaatcg caacagcggt acgcttcggg caactcagag
    13441 catggacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcataa cgtaaataac
    13501 tgacgctcac cgctgtggta acgagttgaa gattgtcgga acaggcaaaa aacgggcgac
    13561 cggcgcgcgc atctaaagcg ccataatacc aggccgccag cagcattccg ctcaacgtgt
    13621 catcatgact tggcgttaat ccggggcctt tacccagcca gtgccgccag tcggtcttaa
    13681 cgccattgag cgcggcctga aaacagtgac gaaactggcg taattcagcg ggcagcggat
    13741 cgcttgccgc catcgccagt ggtccgaaaa gtccggtttc ctccgcgcgt tgcatccatg
    13801 cagctgcaag cggttgagga tgcgaaggcg gcgtaatacg cagcaaacaa taacgctgtg
    13861 gctgtttaac cgtgaaacgc ccgaggcgaa tcccttgagc cacaacctgt ggtcgttcat
    13921 tgccgcataa tccaccgcat aacgcatcga attgcgcgcg gcgaagcacc catcctccgg
    13981 ggccgaaacc actaccctga cgatgcagcg ttaacagttc gccgctttcc gtcatcaggt
    14041 taatcgcccg tcgccacact cccgctaacc gccaggactg gcgataattc ggtgcactac
    14101 ggctggcaag cagaggatgg atgatcgtca ttgcgcgttc ctcaaccaat ccccatgctt
    14161 tcagccagtg cttccagcgc ctgttcaaaa cacgccagcg gtgcccgcac agtgcctgcg
    14221 ccaatctgcc cgatccccgc ctctttatgg gcgataccgg tattgatgag tggcgtaatg
    14281 ccggtttcta ccacgcgacg aatatccagc cccaggcacg cgccctggaa atcccagccg
    14341 gggatctgca actgcatatt gcgctcaagg taaatttccg ccatctcttc agataccgct
    14401 ctggccgctt ccatgccacc cgcaccgaca aagcgcgtta cgccaggcgc tgcgatcatt
    14461 gccgcacctc cgataccaaa ggtttcggta atcgcgctat cgcccatatc cgggttcgcc
    14521 tgctcctgcg agaagccggt gaaaaacaga ccttgcggag tgtttacagg cgcagtaaac
    14581 cagcgttcgc ccagcccgct tacccgaatc ccgaacatat tgccgttgcg ggtcattgcc
    14641 gtgacgatgc tgcctgcgcg gatcatcgcg ccagcatcca tcgccgcctt gcagtaagcc
    14701 atcgcgaggt tgaggaagaa ctgatcggtc acgctgagga aatccatcac ttcggcgatg
    14761 tgctgtttat catgatcgag gcgagcaatt tgtggggcca gcgcacgcat taacagtgca
    14821 gaggaagcaa tattgcgttg atggaactca tcgcccatcg taatgccctg cgccatcatc
    14881 gccgtgagat cgataccgcg ctccatgcgc cccagcgccg cgcttaatac tggcattaac
    14941 acatcgcgca tccagcgatg gcgagtcagg acatcttcgc cgtaagcgcc aaaacgcatc
    15001 actttgccga taccttcgtt gaggttgcag tacgcccggt tgccgtcggt cacgttctcg
    15061 accaccagca tcggcatact ggcagaagta ataccgccca ttggaccgac ggcattcaca
    15121 tggtgacaag gaatgaagtt cacttccccc tgctccagta ttgccagcgc ctgcgcttca
    15181 tctttcgccc agccttcgaa cagacatgcg cccacgcacg cccctttcat gggtccggtc
    15241 atctcctgcc agcgcattgg cggcccggcg tgaagcagtg ttttgccctc gtttagttcg
    15301 ctgataagtg aagaagccgg ttgcacatcc agccagtgtg gacgagcacg acgaatttgt
    15361 tcgataaccg cagcattggc ttgcgccact gatgtaaaca tgggaacccc ttattgcaaa
    15421 cgttctaata aacgagccag ttttttattg ccgccggcga ctggcgacca ttggtaatgc
    15481 acaaccggtt tgctggcgct ttgtagctcc agcgcaaagc tgcgtaatcc gatgttaatc
    15541 acggcgacgt tttccagtaa tgacggtgtg tgttgctgtg tggcagacga gagcggatga
    15601 attaacgctg ccgccagcaa ggtggcttcc ggtagcgaac tcactaccgc gatccccgca
    15661 tcttccagcg tggcgatttg ctgcgagcgg cattgcgggt cacgttcggt acctgtcacc
    15721 gtggcaatgg cgtacagcgg ttggctgtct gaacgcgcgg cacaggcttt ttgccaggcg
    15781 ctcaccagcg aggcagcagg atcggcggtc gcaccgaagc caatcacgac atcaagcagc
    15841 aacacgcgca cttgcggttt agcgccgaga tcggcaatta actggttgcg taaggctggg
    15901 tcgatcatcg gatggggacg cccgacggtg tagaaatcat cgcccagatc aatgatctgg
    15961 tgaccatcgg cgtccagcat catgccgtga tgatgggtat cgtcggcttc cacgccaagg
    16021 tgcccggcaa gtaatcccgc cgcttcggca gcaagcgtac cgccggtata caaaccgcaa
    16081 ataaatccgc tgctgacagg cgctattgcg ttacgtcgcg ccgtgacgcg tgaaagcagg
    16141 caagccaggc gtgcggcctc atccagcgag gaggcaaacc agacattctc gtcgcgggcc
    16201 accgccgggg tataacctaa aaacagcgcc accgtcggtt tgccggttgc tttcatggca
    16261 ttaacaattt tcagacgcac agcttcggca ggtggttttg aaacaaatgc cagcacttcg
    16321 cttttctcgt ctgcactgag catttccagc gctgttagcg cactgatgcc gcccacttca
    16381 cggctgaggt cgcgcccgcc aaggccaatc gcgtgagtaa ttccctcccc tgccagcgca
    16441 atctgcgaac acagctcctg aatcccggta ccggaagcgc caatgacgcc aatattgcct
    16501 tctggcatca cgttagcaaa agccagcggt gtgccggcaa tcatcgacgt accgcagtcc
    16561 ggccccatca ccagcaagcc tttttcccgc gcgcgggttt taagttggat ttcatcttcc
    16621 agcgtgacgt tatcggagaa catcatcacg ttgaggttgc gatccagcgc ctggtttgtc
    16681 agctccgccg catactcgcc agccactgaa atcagcgcca gattggcatc gggtaatttc
    16741 tgacaggcgc tgtcccagcg acgcacctgc gtcaacgcct ggctgctgcc tgacccctgc
    16801 gccagttgtt ttagcgcctc ttcaagctgc tgcataatcg cctgcgcgat ccccgcatcc
    16861 gccgcttcgc tacgaattgc cacgcaaata tcgttcggcg tggcgttatt aaaatcgtca
    16921 tgccagaaac ctgtggtatc taataacgct ttattggcgg gcgtacccat cattacggaa
    16981 acatcatcaa cattttctga ttcgctgagt tttcgtgaaa taatcattaa actgaccgaa
    17041 tcctgaaaac accctttttt aataaaggcg tggatcataa caactcctta aaaattgcac
    17101 ccatggcaat tgcacgagca gtgttaatgt ggcgattacg gtatcgatga caacgagtaa
    17161 ataaggtgat gaatatcaca cagggattat taacaaaaga attcaaataa ccgccaggct
    17221 attacgcatt attttttaat aagcgtggtt gttttaaaaa tcaggttaaa agcgcgaaga
    17281 ttatcaaata aattctaact atattttttg cctgtctgga tcacataatc cagatatttt
    17341 ccctgtatgt taattgcagt catgcttcac accgtcgtta aaaaggaaga cagatgaaaa
    17401 tcagtcggga aacactccac cagctaattg agaataaact ctgccaggct gggttaaaac
    17461 gtgagcacgc tgcaaccgtg gctgaagtat tggtttacgc cgatgccaga gggatccact
    17521 ctcatggcgc ggtgcgcgtg gaatactacg cggaacgcat ttcaaaaggc ggcaccaacc
    17581 gcgaaccgga gtttcgtctt gaggaaaccg ggccgtgctc ggcaatttta catgccgaca
    17641 atgccgccgg acaggtcgcg gcgaaaatgg gtatggaaca tgccatcaaa accgcccagc
    17701 aaaatggcgt tgcggtggtc ggtatcagcc ggatgggtca cagcggcgca atctcttatt
    17761 ttgtgcagca ggcagcccgc gccggattaa ttggtatttc gatgtgccag tccgatccaa
    17821 tggtggtgcc gtttggcggc gcggaaattt actacggtac taacccactg gcctttgccg
    17881 cgccgggaga aggcgacgag atccttacct ttgatatggc gactaccgta caggcatggg
    17941 gaaaagtgct cgacgcccgc tcgcgtaata tgtctatccc ggatacctgg gcggtcgata
    18001 aaaacggtgc accaacaacc gatccgttcg cggtacatgc tctgctcccc gccgccgggc
    18061 caaaagggta tggcctgatg atgatgattg acgtcctctc aggcgtctta ctcggcttac
    18121 cgttcgggcg acaggttagt tcgatgtatg acgatttaca cgccgggcgt aatttggggc
    18181 aattacatat cgttattaac ccgaactttt tcttctccag caaattattt cgtcaacatc
    18241 ttagccagac catgcgcgaa ttaaatgcca ttacccccgc gcccggtttt aatcaggttt
    18301 attatcccgg acaggatcag gatattaaac aacgcaaagc cgccgtcgaa ggcatcgaaa
    18361 ttgttgatga tatttaccag tatttaattt ccgacgcgct ttataacacg tcatacgaaa
    18421 cgaaaaatcc ctttgcgcaa taattattga gacaggactt tcttatgatt acacattttc
    18481 gtcaagccat agaagaaacg ctgccctggc tttcctcttt tggcgctgac ccaacgggtg
    18541 gtatgacccg tttactttat tcgccggaat ggctggaaac tcagcagcaa tttaaaaaaa
    18601 gaatggcagc aagcgggctg gaaacacgtt tcgatgaagt ggggaattta tatggtcgcc
    18661 tgagtggcac cgaatatcca caagaagtgg ttctgagcgg ttcgcatatc gataccgtgg
    18721 ttaacggcgg taacctcgac gggcaattcg gcgcgctggc ggcgtggctg gcaattgact
    18781 ggctgaaaac gcaatacggc gcgccgctac gtacggtcga agtggtggcg atggcagaag
    18841 aagaaggcag ccgcttcccg tatgtcttct ggggcagtaa aaatatcttt gggctggcga
    18901 atcctgacga cgtgcggaat atctgtgatg ccaaaggaaa tagttttgtc gatgcgatga
    18961 aggcttgcgg atttactctt ccgaacgccc cactaactcc gcgtcaggat attaaagcct
    19021 ttgttgaact gcatattgaa cagggctgtg tgctggaaag taatgggcaa tcaattggcg
    19081 tggtgaatgc aattgtcggg cagcgtcgtt atacggtaac gctgaacggc gaatcaaacc
    19141 atgcaggcac cacgccgatg ggttatcgtc gtgatacagt ttacgctttc agtcgcattt
    19201 gccatcagtc ggtcgaaaaa gcgaaaagga tgggcgatcc gctggttctg acctttggca
    19261 aagtagagcc gcgcccgaat acggtaaatg tggtgccggg taaaaccacg ttcaccattg
    19321 attgtcgtca taccgacgct gccgtgctgc gcgatttcac ccaacagtta gaaaacgaca
    19381 tgcgggcgat ttgcgatgaa atggacattg gtattgatat cgatttatgg atggacgaag
    19441 aacccgtgcc gatgaataag gagctggtcg ccaccctgac agaattgtgt gaaagagaaa
    19501 aactgaatta ccgggtgatg cacagtggtg ccgggcacga cgcgcaaatt ttcgcgcctc
    19561 gcgtaccaac ctgcatgatt ttcattccca gcatcaacgg gatcagccat aacccggcgg
    19621 aacgcaccaa tattaccgac cttgccgaag gggtcaaaac gttggcactc atgctttatc
    19681 aacttgcctg gcagaaataa ggagtcataa atgggatatt taaataacgt caccggttac
    19741 cgcgatgatt tactggctaa ccgtgcgatt gttaaacacg gtaatttcgc actgttaacc
    19801 ccggatggtc tggtaaaaaa tattattccg ggctttgaaa attgtgacgc gacaatcctc
    19861 tccacgccaa agctgggtgc ctcttttgtt gattatctgg tcacactgca tcaaaacggt
    19921 ggcaaccaac agggcttcgg tggcgaaggc attgaaacgt tcctgtatgt gatctctgga
    19981 aatatcactg ccaaagccga aggcaaaaca tttgccttaa gcgaaggtgg ctatctttat
    20041 tgcccaccag gctccttaat gacgtttgtt aacgcccagg ccgaagacag ccaaatcttt
    20101 ttatataagc gccgctatgt tccggtagaa ggccatgcac cgtggctggt ttctggtaat
    20161 gccagcgaac tggaacgcat tcactatgaa ggtatggatg atgtgatcct gctggatttc
    20221 ctgcccaaag agttaggttt tgatatgaac atgcatatcc tctcttttgc gccaggtgcc
    20281 agccacggtt atatcgaaac acacgttcag gaacatggtg cctatattct ttccggtcag
    20341 ggggtttata acctcgacaa taactggatc ccggtgaaaa aaggcgatta catctttatg
    20401 ggcgcttatt ctttacaggc tggttatggc gtagggcgtg gtgaagcgtt cagctatatt
    20461 tactcgaaag attgcaaccg cgacgtagag atttaagtca taccactcgc ctgattattt
    20521 ttagctatgt tggattttgc cacggtttat accgtggctt tttttgtagt aaattcaggt
    20581 aaaaaaagat gcgaaataaa tcgcatccgc tttaaaggta agggtcagtt tttaattccc
    20641 tgacctattt taatggcgca ggcaatattt cgcgcgctgt tgaagagatt ggtttcaccg
    20701 ctggcgagca cttcggctaa aggtgccaga cgaggcaaaa tgctgaatac cgcgtcaatg
    20761 ccgtactggt gcaccacttc cacgccatca cccaatacgc cagcaatccc aatcaccggg
    20821 acattaaact gcttcgccac cgacgcaacg cccagcggcg ctttaccgcc tgccgtttgc
    20881 gagtcaatgc gcccttcccc ggtgatcacc agtgctgcgc cctgcaccgc ctgctccagg
    20941 ttgaccgccc gcagtacaat ctcaataccc ggtttaatat ccgcattgag gaataccgcc
    21001 gccgcaatac ccataccgcc cgcagcgccg ccgcccgcca tctggcagac attaatttca
    21061 gtttgctgtt gtaaaacacg ggcgtaattt tgcagcccct gttcaagttc ttcgaccatc
    21121 tccggcgttg cccctttttg cgggccaaac accgccgccg cgccgcgtgc ccctaccagc
    21181 gggttatcaa cgtcacatgc tacttcaata tggcaattcg ccagacgcgg atcgcattca
    21241 tccatctcaa tgcttgccac gcgcgctaaa ttaccgccgt tagccgccag cacctggccg
    21301 tcggcatcaa ggaaacgcac accaagcgcc tgagccatgc ccataccgcc gtcgaccgtc
    21361 gcactgccgc caatgccgag aataatatgg cgaatgccgt tatccagcgc atgacgaatt
    21421 aactcccccg taccaaaact ggaggccagc aacggattac gcttttcagg cgcgaccagc
    21481 atcaggccac ttgctgccgc catctcaatc accgccgttt tcccgtcgcc ggtaaggcca
    21541 taaaaagcat tcactgtttc gcccatcggc ccgcagactt caagcgtcac gatgttgccg
    21601 cccgtcgcgg cgaccatcgc atccaccgtc ccttcgccgc catccgctat cggcaaacag
    21661 atatagttcg catcgggaaa gagggtcgaa aacccggctt taattgcctg acaacatttt
    21721 tctgcactta agctctcttt aaaagagtct ggcgcaatga caatcttcat tatcaatcct
    21781 ttttcaacaa attattcgat gcgttcccac ggaataagat tatccataag aacagccagt
    21841 aaaattccga ccagtaagcc gttactgagc agaggacgca gcgttaatgg aaggtcttgc
    21901 agatacacag gcggtaatgc cattaaaaat atgccgacaa ataacggcaa tgcgagtcga
    21961 taaatattgc gagcggtaaa ggttatttgc tggctaaaca ccagcgcgga aaagagtaaa
    22021 ggcagataag aaaccagcat gaccgcgcta ctcacgggta aagggatact gcaaaacagt
    22081 cgtgtgagtg ccggaaccag cgccaccagc aggcaaataa cgctgccata aataaacgaa
    22141 cgccgcgtgt aatcgcctgt ctgggttaat aaaccaatgg atgaaacaaa cggtgaaaat
    22201 ggaattaccg ccagcggtac ggttatcagc gtcataaatc cggtcgccac aaagctgcga
    22261 cgataacgcg tattcccgcg ccctgctgcg gataaaaaac atccgtgccc cgaatcgcac
    22321 cgtaggtatt gctgatattc accagacctg taatcaccgc cgtcagaata attcccggag
    22381 acaaagcacc tccactgccg agcgggaacc actgccagtg caactcaccg gagagcgagt
    22441 gcgaagaagg aaagcaaaag taccacaaca accagccggt tatggtgccg accagcaggc
    22501 cataacgggc aaaacgttgc ggcaggaaga taatcatccc cagtaccagg cacatcaccg
    22561 ccaccgagag cgcgaacggc ggtaactgaa ttttaaaatt tgggtcggct atgccaaacg
    22621 gcagcccgag catacctttg aaaaagatag tggtcagctg cgcgcccagc atcaacataa
    22681 acaagaccat caccgacggc gtaaataacc gtgccaggcg atggcctaat ccgctaaaac
    22741 caatcaacat cgtcagcacg ccggagagcg caatccccac cgccaggctg gtagcgatat
    22801 cgttgatcgg tgtcccgcgc gatgcttcgc caagggtgat agtaaggatg gttccccacc
    22861 acaggccacc cggcccttcc ataatagcgc gacgatgacc gcagaacgcc tgagcgaagc
    22921 aggccagtgc ggtagcaaga aaagcatatt gcgtgagcgt aagcaggcta ctttgcggca
    22981 actgaaaagc agaaagtagc gtaggaggaa ccacaaccgt gttgcaaaaa ataaagaaaa
    23041 accactgaaa tcctgataac aggctttcgc ggctcactgc aaaattgaac ataaacacac
    23101 cttaaccgga gaatgcccgc cgcaaggacg ggcattgcag gggccagatt actgctgatg
    23161 tttaaggata aattgacctt tcggcgcaac agggaagccc tgttcaatgt cgtaaatcac
    23221 atcaccacgt aagatggttt tcgtgatacg cgcgccaatg gtacggccaa catacgggct
    23281 gactttgtgg cgatattcca gatcgtcatt ggtaagaaca tagctgctat tcggctgaat
    23341 gaagacgaag tcggcatctt ttcctggggc gatacggcct ttttgctgca gaccgaaaat
    23401 atctgctgcg ttagtcgcca ttaatttgcc gaacattggc agagacattc cgcgtttctg
    23461 taccgcttca tcgaacatca cgtccataca gttttgcaga ccggcaatac cgccccatgc
    23521 ttccatgatg ttgccggctt tcatttccgg agggcatggt gagtggtcgg aaaccaggca
    23581 gtcgatttca ccgttaaaca gtttttccca catgcctttc tggttttcca gatcgcggat
    23641 cggcggtgaa cacttcgcca gagtgccgat ttcttcgaac tgatcggtat ccagtacaaa
    23701 gtaatgcggg caggattcac aagtaacgtc ctgaccttcc tgacgtgcac gagtcacttc
    23761 ttcaacgcct tccgggctgc tgatatggca aacgtgcaga cggcaaccgg caactttcgc
    23821 cagatacagc acgcggcgaa tcgcttccac ttcggtaaat accggacgcg aagccacata
    23881 gtcatgggca gttacgcgac cttcacgttt cgcttcttcg cccagtgcgt cacagatcag
    23941 cgcgttttcg cagtgcacca gcaccggctg ccccagttcg cccagcttct gcgcaccttt
    24001 gaagaactgc cagtcgttta catcacggaa gtcgttgtcg ataccgcgat cgccacaggt
    24061 cgcaacgaag catttgaagc cgacaacgcc cacttcatcc agctcatgca gacggtcgat
    24121 gttgtaagac accaggccac cgagttgtgc cgcatcgata gtcagcttgc ctttagcggc
    24181 atcgaacttc agttcaattg aagcgcggtc aaccgttgca ggcagctggt tgagcggcat
    24241 ttcgatcatg gtggtgatac caccttttgc cgctgcgcga gtaccggttt cataaccttc
    24301 ccagtggcta cgacccggtt cagaaatatg ggtgtgcgca tcaaccatgc ccggcgaaac
    24361 caccagacca gacgcatcca taacttcttt tgcatcgccc agatcctgac cgatagcagc
    24421 aattttcccg cctttaacgg cgatatctac aacgcgagct tcgttttcta aaataacggt
    24481 gccgttttta atgattaaat caaaagacat aacaaactcc ttgtaaaata cagattggtt
    24541 tttaactcaa tgttcaatat cgggattaat taacctatgg ttttttgctc tcctgttttt
    24601 tctgctgttg tacgtttctt taataaggcg taggccgcaa aggcgacgat gacgccgaca
    24661 aaccatgaaa cacgcgataa cggttccata aagtgaataa acttaccgcc aagagataaa
    24721 ataacggcca ccagagttac tgaaaacgca gtgaggttaa aaccgttatc gtaatattta
    24781 taatcgccag gtgcggtata cagttcatca agattaattt gtccgcgcat caccacaaaa
    24841 taatgcgcca tcatgacacc aattaccgga ccaagcattc cgccgataat atcgaggaaa
    24901 agataaatgc tgtcctgatt ttccattaat ttccacgggc agatcagcaa gctgataata
    24961 ctggcaatca gtacgccgtt tttataggtc agttttgtcg gtgcaatggc ggcaatctga
    25021 taaccggctg gaataatatt accggtcgcg ttagtggaga tagttgtcat cagaatgacc
    25081 agtaccgcaa agaacgaggc gaacaggctg tcccaacgct gaacaatatc cagcacgttc
    25141 caggtatcag cgccgtagtg aatactggct ccggcaataa tacatacccc ggcgaccgca
    25201 aacagaatat aggccacaac taaacccagc gtttgcccca gcgcctgctc acgaaacgag
    25261 tgggcgtttt gcgtaaagtc ggatgcgctc accgccggtg ccgcccagac cgcaactacc
    25321 gcgttaatca ccaccaggaa caggaagcca ccgttttctg ctttctgaat accgctcgga
    25381 atgtagtcaa agattggacc gatcccgacc agtgaaatcg cccaaatcgc cataccgccg
    25441 aaaacgatat agatgcacgg gttaagaatg gcagtgaatt tatttaaaac tttgccaccg
    25501 ccaaaaccta taccaacgtt gaccagccag aagattaaga aagtaattaa gcccggtaga
    25561 gaaaggccta acagagtgaa atcaccaccg agagttaaaa atcccggcca gattttgcca
    25621 atcagaatca agcaggccag tgaccccgcg taacattgca aaccaaacca catgatggcg
    25681 gcaattccgc cccttaataa tccgggaaac agtgcaccac gtacaccgta agaagcacgc
    25741 aggatcatgg caaaaggcac accgtattta ctgcccgcag caccgtttaa taccattacc
    25801 gcggcaatga aaaaggcgct gaggataatt gccagcataa tactaaaggt agacaagccg
    25861 agaataaaaa agccgccgac catcacataa ttgggaacgt tatgaaccga acccatccat
    25921 aaggtaaaat agttaaatgt tttccaggtc cgctggcttt gcgttttcgg caatagatct
    25981 tcgctatagc cgcgttgctg gaatagtttt ctctgatgtt ccataaatcc ctcttaattt
    26041 cacgtatcga ataacgttgt tttaatctag ctatgaggaa caaataaaat aaaaatatta
    26101 tttttaatac tgaagcaaca tctttggctg ctttcttttt taatattgct ggccgattca
    26161 ttttctgata tcggccattt ttattgcggg tatcaggcca gtttatggtt agccattaat
    26221 tccagcgcct gcactaacgc agagtgatcc aactggctgc caccgtttgc cgcgcaggta
    26281 ttaaataact cctggcaggt cgcagtgttt ggcaggttca gcgctagcgc tttcgcactt
    26341 tgcagtgcca ggttgagatc tttctggtgc agcgcaattt tgaaacctgg attaaaagtg
    26401 cgtttaatca tgcgctcacc gtgaacttcc agaatacgtg aggaagcaaa gccgcccatc
    26461 agcgcctggc gcacacgtac cgggtccgca ccggcttttg aagcaaatag cagggcttca
    26521 gaaaccgctt caatattgag cgccacaata atctgatttg ccactttgca ggtttgacca
    26581 tcgccgttac cgcccacgag ggtgatattt ttaccgagca gttcaaacag cggtttaaca
    26641 cgttcaaata ccgcttcatc accgccaacc ataatcgaca acgtcccttc acgcgcaccg
    26701 atttcaccgc cggagactgg cgcatcgaga taatcgccgc ccagttcatt cacctgacga
    26761 gcgaaacgct tagtttcaat cggggaaatg gagctcatat caacaatggt tttgcccttc
    26821 agcgaggctt tggtacaacc attttcaccg aacagaactt cttcaacctg tggtgtatct
    26881 ggcaccataa taaaaatgat gtccgatgct tccgttacct ggcgcgcagt ttcaacactg
    26941 acggcaccca gtgacagtaa ttcatcagca accggaccaa tggttgtgac atgcagttga
    27001 tgaccggcac gcgccagatt aatggccatc ggtgtaccca taatgcctaa gccaataaat
    27061 cccagtttca taattaacct cttttaaatt tcgcttttcc tgaattcaga caacacgatc
    27121 ccctgccaac ttagcggcct aaaaaagcat tgcctgaaca gcgttacgtt ttaacggtac
    27181 ggatccatcc agcgtaaacc ggcttccgtg gtggtttggg gtgtatattc acacccaacc
    27241 caaccgttgt agtcagaatt ttcgattacc ttaaagagat aatcataatt aatttctccg
    27301 gtccccggtt cgccgcgatg cggattatca gcaatttgca ggtgaccaat tttatcggcc
    27361 cactgagtca tggtgttggt taattcacct tccatccgct gcatgtgata aatgtcatac
    27421 tgaattttca aattgcagca accaacatca tcaatcaatt tcagcgcctg ccgagttccg
    27481 gtgagatgga aaccaggaat atcaaaatgg ttaataggtt caatcagtaa taaaatatct
    27541 tctttcatca gcatattcgc ggcataacgc aggttttcta caagcgttgc gtgaatctgt
    27601 tcactgctga aaccagccgg cgttttaccg accagacagt taattttttt attacccagc
    27661 gcacgggcat aacgaatcgc tgctgctacg ccatcccgaa actcttcttc acggccagga
    27721 atacaggcaa taccgcgctc ccccgccgcc cagtcacctg ccggtaaatt gtgcagcgta
    27781 tgttcgagtt tattactcgc cagcacctgt tttaattctt caatgtcgta gtcataagga
    27841 aacataaatt caacgccgcg aaaaccacac tgcgcagctt tctcaaaacg ggcgagaaaa
    27901 tcatattcac caaataacat cgataaatta gcagagaaac gtaacattat ttatctccct
    27961 tattcatagt gcatgaagca ggtttcagtc ggtgcgtccg ctgcgttatc ggcgatatct
    28021 tcaaattcca tgacgttatc cagttcgctg cccatcgaaa tattggtcac acgctcaaga
    28081 ataacttcca cgactaccgg tacccgatat tgcgccatta aggctttcgc ctgttcaaag
    28141 gctggcgcaa tatcttccgg tttgaagacc cgaatagctt tacaacctaa accttccgct
    28201 acttttacgt ggtcgacgcc gtaaccgttc acttcactgg agttaatatt ttcgaacgcg
    28261 agctgtacgc agtaatccat gtcaaaggcg cgttgtgact gacgaatcag ccccagataa
    28321 gcgttattga ccagtacatg gatgtacgga atgttgaact gtgcgccaac ggctaattct
    28381 tcaatcagga actggaagtc gaagtcaccg gaaatcgcca ccacattccg ttctggatca
    28441 gcagcacaca cgcccagcgc cgccggaatg gtccagccca gcgggcctgc ctgaccacag
    28501 ttgatccagt gacggtcttt aaagacatgc agcatttgtg ccgcagcgat ttgtgacagg
    28561 ccaatggtgg tgacataaca aacatcgcga ccaaaggctt tgttcatctc ttcatacacg
    28621 cgctgcggtt tcaccggcac gttgtcgaag tgggttttgc gcagcaaagt gcgtttgcgc
    28681 tgctggcagt cggcgaccca ttctttacga cacggcagac gacccgcttt ttgcatctcc
    28741 tgcgccactt caaccagcag tgtcagcgcc gctttagcat cagagacaat gccgagatcc
    28801 ggacacagca cgcgaccaat ttgcgtcggc tcaatatcga tatgaacgat tttgcgccct
    28861 tcggtgtatt tctctaccga accggtatga cggttagcaa aacggttacc gataccaaac
    28921 accatgtcgg acgccagcag cgttgcgtta ccgtaacgat gcgcggtttg cagacccacc
    28981 atcccggcca tcagttcatg atcgtccggg atacagcccc agcccatcag cgttgggatc
    29041 accggaacgc tggtcagttc agcaaactgt tgtaacagtg cagctgcgtc agcattaatt
    29101 accccgcccc cggcaacaat caccggacgt tcggcctgga ttaacatttc tacagctttt
    29161 tcgatctgca tacggctggc agcaggtttg tagaccggca gcggttcgta catatcagga
    29221 tcaaactcga tttccgcaac ctgaacgtcg aacggtaaat ccaccagtac cggacccgga
    29281 cgaccagaac gcatcaggtg aaatgcctgt tgcaacacac gaggcaccag cgccgcttca
    29341 cgtactgtaa ccgccatttt acttaccggc ttggcgatag cttcgatatc aacagcctgg
    29401 aaatcttctt tatgcagacg ggcgcgcgga gcctggccag taatacacag aataggaatg
    29461 gaatcagcag aagcagaata gagcgcggtg atcatgtccg tgcccgcagg accggaagtc
    29521 cccagacata cgccgatatt ccctgccgtt gcgcgggtat aaccttccgc catgtgcgaa
    29581 gcaccttcca catgacgcgc cagaatgtga cgaataccgc cgtgcttacg catcgctgag
    29641 tagaacggat tgattgcagc tcccggaacg ccgaaggcgg tggtgatacc ttctttctcc
    29701 agcacataca ttgccgcgtc aacggctctc atttttgcca ttttattcca acctctatta
    29761 attggaaaaa ttttccaact ttcaaatttt gtcaaaaaaa cccgctccgc agagcgggat
    29821 gcgacagaca ctatggatgt gctttcagtc ccaacgccgt gctgatatcg cgggcggtgt
    29881 ctctgaccag ctcaccctga ctgacaaaac gatcttctgt cagtcttgat gaaggcccgg
    29941 agatggagat agcggcaaca acgctaccga catcatcgta aattgctgaa gctatgcaat
    30001 tcagacctac aacatgctct tctttatcta cggtatagcc cagttcacgc gcttgttcca
    30061 ggtccttcag caaggtgggc atatccacaa gcgtagttgg cgtaaactgc tgcaaaccgg
    30121 tttgcagaat gatgctcatc aactcctctt ccgccagcgg ataaagcaac gccttgcccg
    30181 caccggaagc atgcagcggc agacgactgc ccagtggcgc acacatcctg accatcgatt
    30241 tacactctaa ctgaccaatt aataccgctt cattgccgtt acggatcgcg acattgaccg
    30301 tttcgccgga aagtaacatc aggcggcgca taaacggccc ggcgacggag aggacatcgc
    30361 ggttatggat gtacgccgca ccgacgttaa agacacctaa tcctatatgc caccagccta
    30421 actgactgtc ctgatagaca aaatccgctg cctgtaaaac cttcagcaag cgaaaggtcg
    30481 tagagagcgg caaatccaga ttgagagaaa tatcgctaac cgacgaactt cccccacttt
    30541 tttccagata ttgcagaatc gcaattcccc gctctaacgc ctgtgcgccc ttctgtgcca
    30601 caggctccgc ctgtcctggc ctgccgcgcc gtctaacttc cgtcatacct acgttaactc
    30661 cgacctacaa gtgagcataa acaatgaaca attagtttat cttatgcaag tcggttgcag
    30721 gcgtcacgca aaacagagtt cctgttcagg aatactttca acatcacagt tgtcactgcc
    30781 ccgcgatcga tggtcagaaa atcggtgacg cgctgccagg cgaaaagtgg gtgatgccag
    30841 acgttacggt ggtaattcac tccctgttcg ccgttggtaa taaacgcccg cagggttgac
    30901 aggtctggtt tgtcgtcacc taacgccacg accaccacaa acacctcacc tttcatcggg
    30961 ataaaggcct gagtacccag cggatgacgt tcgagttcgt gaatggtcag cggcagattc
    31021 gccggttgcg cgcggttaat gctgataagc gtacggtctt gctcaagaat ctcaaccagc
    31081 gccaaatcgt ggtaacgctc caccaggcca ttgttaatat ggaaaaaatc ccgttgctgc
    31141 gtttcgatta cgtcgccata agcactaaag gcttcctgac ttaacagtaa tacctgaagt
    31201 ttcatctttt tgtccttgta acataattcc gttcaaccac cgccgaccgg tcagcttggt
    31261 ttcaataaca ggcaatctaa aaccttaaga gaacaacatc aatacaaaag ttggaatatt
    31321 tttccaaaac tagatttagt ccacaaattg aacagggtta tcatgtaaaa atactgataa
    31381 atcagaggat aagaaaatca cgcaacttgc ggatttaaaa ggggatacct gaagatgttc
    31441 gatccagaaa ccttgcggac tttcattgcg gttgctgaaa caggaagttt ttcaaaagcg
    31501 gcagaacgat tatgtaaaac cacggcgacg atcagttatc gcattaaact tctggaagag
    31561 aataccggag tagcgctgtt tttccgcacg actcgcagcg tgacgttgac agcggctggc
    31621 gagcatctac tttcccaggc cagagactgg ctgagctggc tggaaagtat gccaagcgag
    31681 ctgcaacagg tgaatgatgg cgtggaacgc caggtgaata ttgtcatcaa caacctgctc
    31741 tacaaccccc aggccgtcgc ccagttgctg gcgtggctga atgaacgtta cccctttacc
    31801 cagtttcaca tctcccgaca aatctatatg ggcgtctggg attcgctatt atacgaaggt
    31861 ttttcactgg ctatcggcgt cacgggaacc gaggcgctgg caaatacctt tagtcttgat
    31921 cccttaggat cggtgcaatg gcgctttgtc atggcggcgg atcatccgct ggcgaacgtt
    31981 gaagagccgc taacagaagc gcagttgcgg cgctttccgg cggtcaatat tgaagacagc
    32041 gcccgcacct taaccaaacg cgtcgcctgg cgattgcctg ggcaaaaaga gattattgtt
    32101 cctgatatgg aaacgaaaat cgccgcccat cttgcgggcg ttggcattgg ttttttgcca
    32161 aaatcgcttt gccagtcaat gatcgataat caacaactag ttagccgggt aatcccaacg
    32221 atgcgccctc cttcgccatt gagtctggca tggcgcaaat ttggcagcgg caaagcggta
    32281 gaagatattg tgaccttgtt tacccagcgc aggccggaaa tcagcggatt tttagaaatt
    32341 ttcggcaacc cacgcagtta aaaatcaagt ttccttgcgc gttggcgcgt aaaatagcgc
    32401 gcaagctgat ttgaggttca cttttaacta tgcaagagag acacacggaa caggactatc
    32461 gtgccctgct gattgctgat acgcccatta ttgatgttcg cgcccctatc gagtttgagc
    32521 aaggcgcaat gcccgccgct atcaatctgc cgttaatgaa taacgatgaa cgcgccgccg
    32581 ttggcacctg ctataaacag caaggctcag acgcagcgct ggcgctgggg cataaactgg
    32641 tggcgggtga aattcgtcag cagcgcatgg acgcctggcg ggcagcgtgc ctgcaaaatc
    32701 cgcaaggtat tctctgctgc gcccgtggcg gtcagcgctc acatattgtg caaagctggt
    32761 tgtatgcagc ggggattgat tatccgctgg tggaaggcgg ttataaggca ctgcgccaga
    32821 ccgcgattca ggcgactatt gaactggcac aaaaaccgat agtgctgatt ggcggttgta
    32881 ctggcagcgg taaaacgctg ttagtgcagc aacagccgaa cggtgttgat ctggaagggc
    32941 tggcgcgcca tcgcggttcg gcgtttggtc gcacgttaca accacaactt agccaggcga
    33001 gttttgaaaa cctgctggct gccgaaatgc tgaaaaccga cgcccgtcag gatttgcgcc
    33061 tgtgggtgct ggaagacgaa agccggatga tcggttcgaa tcacctgccg gaatgcctgc
    33121 gcgagcgaat gactcaggcg gcgattgcgg tggtagaaga tccgtttgag atccgtcttg
    33181 agcgcctgaa cgaagagtat ttcttgcgta tgcatcatga ttttacccac gcttatggcg
    33241 acgaacaggg ctggcaggaa tattgcgaat acctgcatca cggactttcg gcgattaagc
    33301 gtcggctggg gttacagcgc tataacgaac tcgctgccca gctggatacg gcgctgacaa
    33361 cgcaactcac caccggcagc accgacggtc atctggcctg gctggtgccg ttacttaaag
    33421 aatattacga cccaatgtat cgctatcagc ttgagaaaaa agcggaaaaa gttgtctttc
    33481 gcggtgagtg ggcggaagtg gcggaatggg ttaaggcgcg gtaatgaggg acgtatttgt
    33541 attagtcgcg acttgatctg acacatggcc ttgaaaggtt gagagttacc ggttttgata
    33601 tgagtgtcga atccttatcc aaaacaagag gtaactctca tgcttcatac tgccaatccc
    33661 gtcatcaaac acaaagccgg tttgctcaat ctggctgaag aactcagcaa cgtatcaaaa
    33721 gcctgtaaaa tcatgggcgt ttcgcgtgat acgttttatc gttaccgcga actggtcgct
    33781 gaaggcggtg tggatgcgca gattaatcgt agtcgccgcg ctcctaacct taagaaccgt
    33841 accgatgagg caactgaaca ggctgttgtt gattacgccg tcgctttccc gacacatggt
    33901 cagcaccggg ccagcaacga gctgcgtaaa cagggggttt ttatctccga cagtggtgtc
    33961 cgttctgtct ggttactcca taaccttgag aatctcaaaa gacgatactg aacaaatttt
    34021 atcaggtgac gttccgtaaa aagttgtatg gagttctcga tgcagtcggc gcgactatca
    34081 gtacccccgc tgaaccctgg actatcgggc cgccgtactg gggcatgtca ccctggcgtg
    34141 ccgctggttt tccgctaatg gtttatttcc tctttctttc ccggcaagaa tttattttaa
    34201 tccggaagga ttatttattt tcagataggg actcacatta aacacgtaac attttaatta
    34261 atgcatggct gaatatgtat tacacaatgt cagatatcgt aaaagaccag tgcaattcta
    34321 tgtaaactct ctgactgttc attttatttg ttgtttcagg gtcggaactc accctcaata
    34381 aacaactgaa tacgagtaga tattcatcgt ctgagctata tggctttaca caatagccga
    34441 catgtctttt ctcagtcagg aattttactt ttcctcacta cgaagttgac gacttcgata
    34501 tgggatagac tcttaattca agcaattact ctgcacaagc atcaggcaat ccagctccaa
    34561 tatttgttat aaaataatgc gtaacattaa tttcaccaac aaaaaaaagg tttacaccat
    34621 ccccttgccc tggtatctta ccatatttac accacacttt ttttgtatct ggatcattct
    34681 ttgatacgat tttcgccact tgttccctgg ccctcactgt caatgttttc ttaaacctac
    34741 ccccatcatc atttgttagg gaaaaataag aaaaaaaata atcatcttct acatttgtgt
    34801 atattgatat atccaccgct acgtgcggaa aaaaaatctg ggagccatgt aaaaactcca
    34861 tcattttctg gtattgccat atagtttatg taatgtttac ttacaagaga ttcatctttg
    34921 tataaataaa gataagtaat tacgcataaa acaacaatga ttataatagc aaaaataaat
    34981 attatcatct ttgatagatt acttgagata gccagcatct tgtaaagcct ttatcgtttt
    35041 tttatgctct ggattaatat aatcactaca tctatctgag caatctgttg ttgatggaca
    35101 tgtcaaccca tggtcattta cagccaagtc ttttttatta tcttcaatca tttcagaatg
    35161 ggatagcttt acttttctgc cgtacatacc gaacagattt aacccgtaat ctctaatctc
    35221 tttttcataa cccagccctt tcgccgatcg gctaacacca gcgtcattta atttagacac
    35281 tcgacaaaat gccatacaat ggaaaaattg atcagtccca cctaaattta atctcttcat
    35341 atcttcataa gtatcagata atatatccca tgctctttga tggtttagtt gatcttttct
    35401 tcttattagc gctacatctg cgggacttaa ccctaatgga tcaataccat tcaccggatt
    35461 cagcgggtac gcatacagac tccatccccc cctccagccc tatcgggtcc tgagtgatat
    35521 accgcccctg caacggatcg tagtaccggt tccggtt
//
LOCUS       DAZGJW010000035        35354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZGJW010000035 DAZGJW010000000
VERSION     DAZGJW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.035"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..670)
                     /locus_tag="ACHKIK_001755"
     CDS             complement(<1..670)
                     /locus_tag="ACHKIK_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO9082942.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWVLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFH"
     gene            complement(873..1274)
                     /gene="nikR"
                     /locus_tag="ACHKIK_001756"
     CDS             complement(873..1274)
                     /gene="nikR"
                     /locus_tag="ACHKIK_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO9082943.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(1280..2086)
                     /gene="nikE"
                     /locus_tag="ACHKIK_001757"
     CDS             complement(1280..2086)
                     /gene="nikE"
                     /locus_tag="ACHKIK_001757"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO9082944.1"
                     /translation="MTLLNISGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(2083..2847)
                     /gene="nikD"
                     /locus_tag="ACHKIK_001758"
     CDS             complement(2083..2847)
                     /gene="nikD"
                     /locus_tag="ACHKIK_001758"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO9082945.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2847..3680)
                     /gene="nikC"
                     /locus_tag="ACHKIK_001759"
     CDS             complement(2847..3680)
                     /gene="nikC"
                     /locus_tag="ACHKIK_001759"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO9082946.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMTATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3677..4621)
                     /gene="nikB"
                     /locus_tag="ACHKIK_001760"
     CDS             complement(3677..4621)
                     /gene="nikB"
                     /locus_tag="ACHKIK_001760"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO9082947.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4621..6195)
                     /gene="nikA"
                     /locus_tag="ACHKIK_001761"
     CDS             complement(4621..6195)
                     /gene="nikA"
                     /locus_tag="ACHKIK_001761"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9082948.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(6306..6893)
                     /gene="acpT"
                     /locus_tag="ACHKIK_001762"
     CDS             complement(6306..6893)
                     /gene="acpT"
                     /locus_tag="ACHKIK_001762"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO9082949.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWH
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6948..7997)
                     /locus_tag="ACHKIK_001763"
     CDS             complement(6948..7997)
                     /locus_tag="ACHKIK_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO9082950.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            8129..9346
                     /locus_tag="ACHKIK_001764"
     CDS             8129..9346
                     /locus_tag="ACHKIK_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9082951.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVTIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(9350..9907)
                     /gene="dcrB"
                     /locus_tag="ACHKIK_001765"
     CDS             complement(9350..9907)
                     /gene="dcrB"
                     /locus_tag="ACHKIK_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO9082952.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9980..10645)
                     /locus_tag="ACHKIK_001766"
     CDS             complement(9980..10645)
                     /locus_tag="ACHKIK_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO9082953.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10866..11111
                     /gene="tusA"
                     /locus_tag="ACHKIK_001767"
     CDS             10866..11111
                     /gene="tusA"
                     /locus_tag="ACHKIK_001767"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO9082954.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(11213..13411)
                     /gene="zntA"
                     /locus_tag="ACHKIK_001768"
     CDS             complement(11213..13411)
                     /gene="zntA"
                     /locus_tag="ACHKIK_001768"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO9082955.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEEKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13485..14111)
                     /locus_tag="ACHKIK_001769"
     CDS             complement(13485..14111)
                     /locus_tag="ACHKIK_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO9082956.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            14252..14611
                     /locus_tag="ACHKIK_001770"
     CDS             14252..14611
                     /locus_tag="ACHKIK_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO9082957.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14614..14883)
                     /locus_tag="ACHKIK_001771"
     CDS             complement(14614..14883)
                     /locus_tag="ACHKIK_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO9082958.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14873..15469)
                     /gene="rsmD"
                     /locus_tag="ACHKIK_001772"
     CDS             complement(14873..15469)
                     /gene="rsmD"
                     /locus_tag="ACHKIK_001772"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO9082959.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15619..17115
                     /gene="ftsY"
                     /locus_tag="ACHKIK_001773"
     CDS             15619..17115
                     /gene="ftsY"
                     /locus_tag="ACHKIK_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO9082960.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVAEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            17118..17786
                     /gene="ftsE"
                     /locus_tag="ACHKIK_001774"
     CDS             17118..17786
                     /gene="ftsE"
                     /locus_tag="ACHKIK_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO9082961.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17779..18837
                     /gene="ftsX"
                     /locus_tag="ACHKIK_001775"
     CDS             17779..18837
                     /gene="ftsX"
                     /locus_tag="ACHKIK_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO9082962.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILMLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            19082..19936
                     /gene="rpoH"
                     /locus_tag="ACHKIK_001776"
     CDS             19082..19936
                     /gene="rpoH"
                     /locus_tag="ACHKIK_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO9082963.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            20207..21310
                     /gene="livJ"
                     /locus_tag="ACHKIK_001777"
     CDS             20207..21310
                     /gene="livJ"
                     /locus_tag="ACHKIK_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO9082964.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(21509..21892)
                     /gene="panM"
                     /locus_tag="ACHKIK_001778"
     CDS             complement(21509..21892)
                     /gene="panM"
                     /locus_tag="ACHKIK_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO9082965.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGL
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            22316..23425
                     /gene="livK"
                     /locus_tag="ACHKIK_001779"
     CDS             22316..23425
                     /gene="livK"
                     /locus_tag="ACHKIK_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO9082966.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            23473..24399
                     /gene="livH"
                     /locus_tag="ACHKIK_001780"
     CDS             23473..24399
                     /gene="livH"
                     /locus_tag="ACHKIK_001780"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO9082967.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            24396..25673
                     /gene="livM"
                     /locus_tag="ACHKIK_001781"
     CDS             24396..25673
                     /gene="livM"
                     /locus_tag="ACHKIK_001781"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO9082968.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            25670..26437
                     /gene="livG"
                     /locus_tag="ACHKIK_001782"
     CDS             25670..26437
                     /gene="livG"
                     /locus_tag="ACHKIK_001782"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO9082969.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            26439..27152
                     /gene="livF"
                     /locus_tag="ACHKIK_001783"
     CDS             26439..27152
                     /gene="livF"
                     /locus_tag="ACHKIK_001783"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO9082970.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            27548..28864
                     /gene="ugpB"
                     /locus_tag="ACHKIK_001784"
     CDS             27548..28864
                     /gene="ugpB"
                     /locus_tag="ACHKIK_001784"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO9082971.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            28962..29849
                     /gene="ugpA"
                     /locus_tag="ACHKIK_001785"
     CDS             28962..29849
                     /gene="ugpA"
                     /locus_tag="ACHKIK_001785"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO9082972.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            29846..30691
                     /gene="ugpE"
                     /locus_tag="ACHKIK_001786"
     CDS             29846..30691
                     /gene="ugpE"
                     /locus_tag="ACHKIK_001786"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO9082973.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            30693..31763
                     /gene="ugpC"
                     /locus_tag="ACHKIK_001787"
     CDS             30693..31763
                     /gene="ugpC"
                     /locus_tag="ACHKIK_001787"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO9082974.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            31760..32503
                     /gene="ugpQ"
                     /locus_tag="ACHKIK_001788"
     CDS             31760..32503
                     /gene="ugpQ"
                     /locus_tag="ACHKIK_001788"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO9082975.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLWILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(32490..32930)
                     /locus_tag="ACHKIK_001789"
     CDS             complement(32490..32930)
                     /locus_tag="ACHKIK_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO9082976.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            33050..34792
                     /gene="ggt"
                     /locus_tag="ACHKIK_001790"
     CDS             33050..34792
                     /gene="ggt"
                     /locus_tag="ACHKIK_001790"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO9082977.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(34830..35114)
                     /locus_tag="ACHKIK_001791"
     CDS             complement(34830..35114)
                     /locus_tag="ACHKIK_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO9082978.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         8199 a         9208 c         9890 g         8057 t
ORIGIN      
        1 ggtggaacgt ctgtgtgcgc ccgaagcggt ccaccagccc ggtcagtacc cggtacggcg
       61 gcagcggcgc aggcagcacc tcatccgctt ccggcacccg ctcacaccag ccgagtaccc
      121 accacggccc ctgcggactg tttgtcgcca gataacgatg cggactcagg cgaagctctt
      181 ccggcagcgc ctgccagagt gcggccagcc ggtgaccttc atccagtttc gccacaccgc
      241 cgcgcaccag ccagagtgat tcgctgcggc tgtaaccgtc ctcaccggga aacaggtgct
      301 caaaatacag gcttctgccg ccgttatcac tgagtatcag tgtgttatcg cgcagctgta
      361 agcggatatc cgcaggcatt ttccagccgg ggccgaggct ccccaccggc gcgggcgttt
      421 ttgtccggta actgctgtag gtgcgggaga gaatgaacgg cagcgggccg ggcagggcga
      481 tgtcggtttc accgggaagg acctttgcac ccagcagcgg attgaccgga tggccggacg
      541 tcaccccgcc ggggcacacc gaacaggcca cgccggtggg ggcaccaata cgtaccccgg
      601 ctgaaccctg aacaatgcta ccgccatact gcgtcatgtc accctgacgc gccgccggtt
      661 ttccgctcat cgtaaaatcc ttttctatta ttttaatgac agtatcttca tacgaaaaag
      721 cgaagaaaaa cttgtggtca caatgaattg aagattttta ggaatatatt tagaacgtta
      781 caaaagagaa ggttcggata gggtatttag agaggaatca ggtgtgctag tggccctttg
      841 ttgatcgttc accaaaggac ccaataatag actcaatctt ccttcggcaa gcactgcaaa
      901 tgcccgtgcc gcacgccgcg ctgggcgata acgtcatcgg caaaatgctg cacgtcgccc
      961 atatcacctt tcagcacggc gatttccaga cagtcgtcgt gattgatatg cacatgcagc
     1021 gtggcgacgg agagatcgtg gtgatggtgc tgggtggaga caatgcggct ggctaagtcg
     1081 cgtttttcgt gttcatacac ataggagagc accgcgaaac cttgcgtgcc gtgctgctga
     1141 gtggcctcct gggccagagc gctacgcaga atatcgcgga tagcttcgga acgattgtta
     1201 taaccacgac gctggctcag gctgtccagc gtctccagta aatcgtcatc aagcgtgatg
     1261 gtgactcgtt gcatttgcgt taaacctttt ctgtggtgcg acggcgcacg gggaatgcgg
     1321 gtaataccgc gttttgtagc acacgtccgg cgtcagagga aaaggttaat ttatctccca
     1381 ccacctgggt ttcgacgatt tgtccgttgt ccatcaccat cacccgctga cagaagcgtt
     1441 ccaccaggcg taagtcgtgg gtgatgaaca ggcaggcggt gccaaactgt tgttgtagct
     1501 ttttcagcag gcgaatgacg cccgcctgta acacgagatc aaggttagaa acggcttcat
     1561 ccagaatcag tagtttcggt tcgaccgcca gcgcgcgagc caggcagacg cgctggagct
     1621 ggccgccgct taactgcggt gggcgtttgt cgagaacgct gtcatcgaga tcgaccgcct
     1681 tcagcatttc gctggcgcgc gccagttgtt cggatttttt cagtgatagc aggtggcgca
     1741 tcggttcacg caggatctcg cgcacggttt tgcgcggatt cacggcgctg atggagtcct
     1801 gaaataccat ctgaatatcg cggcggaacg ctttacgctg ggcgcgattg agtttcgcca
     1861 gcggttcgcc acgccagcta atattcccct gcgcgggcga ttctaaaccc accagcaacc
     1921 gcgcgagggt acttttcccg cagccgctgc gccccagcag ggcgacggtt tcgccgcttt
     1981 tcagggtcag ggaaacgtta ttcagcaccg cctgatgttg atgttttccg ttaaatccac
     2041 cgtgcgcata gtgatgggaa aggccggaga tgttaagtaa agtcatgatg ccagctccat
     2101 accgtagagg gcgagatgag cggaaaccag gctgcgcgtc actgtatgtt tgggggcgtt
     2161 aaacagcgtt tctacatcgc cctgttcgac aatcttaccg tcagacatca ccgccacgtc
     2221 atccgccaga cgcgccacaa cgcccatatc atgggtcacc agcagcattc ccggcgcttg
     2281 tttttgcata atgctttcca gcagatcgag gatgcgcgcc tgtgctacca cgtcgaggtc
     2341 ggtggtcggt tcatcggcga tgataaacgg tgattcacac agcaccgcca tcgcaatcat
     2401 catgcgctgc aacatgccgc cgctcatctc gaacgggtac agcttcagca cgcgcgcggc
     2461 gttttccagc cccaccgctt ctatggcagc ggtaagcgta gcgtcatcgg cgggtttccc
     2521 taacgccagg caggtttccc gcgcgtgggt gtgcatggtg tgcagtggat taaaggcgct
     2581 gcgcgggttc tgcatgatgg tggcaatttt gatgccgcgc agggcgcaag gcgaaaccgg
     2641 tttgccatcg gctaaaattt cccccgccgt ctggcgaacg ccagcgggca aaatgcccag
     2701 cgtcgcggcg caggttaatg atttcccgct gccgctaccg ccgactaacg ccagcacgcg
     2761 cccgcgttgc agggttaacg atacaccgtg taccagcggc tgcgcggcct gtagcgcgat
     2821 attacgtagt tcaatctgtt gcggcattag tgtgcgtgct ccgtcaccag atgagggtcc
     2881 agatgatcgc gcagtgcgtc acccaccagg ttaaaggcca tcacgctgat aaacagcgcc
     2941 agccccggcc agaacatttg cagcggctgg gtccagatat actggcgcgc gtcgttaatc
     3001 atcacgcccc attcggcggt cggcgcggtc acaccgaggc caaggaaaga catccccgcg
     3061 acgtgcagca tcatatggcc gatatccagc gttgccagca ccagcagcga agggatcacc
     3121 gcgcctgcca gatgatcgac aaacacccgc acatggcccg caccggaaag ccgtgacgcc
     3181 agcacaaact cgcgttggcg tagtgaaatc accaggctgc gcaccatgcg tgcataccac
     3241 gcccagtgcg acagggcgat ggcgataatt acgttggtca gcccggtgcc gagcacgcca
     3301 accataaaga acgacagaat cgaggtcggg aaggtcataa acatatcggc gacgcgcatg
     3361 gtggcctgat caacgcgccc gccaatcaac ccggcgctgc cgccaataac cagccctaat
     3421 gtcagcacca gcagcaggca ggccattacc gaaccgagcg acacgcgggt cgctgtcatc
     3481 agccgcgaga aaatatcgcg acctaagtga tcggtgccca gccagtgctg cgcatccggc
     3541 gaaagcaggc gcgacggcaa atcaatcgcc tgggggtcat acggcagcca ccactggctg
     3601 gtgagcgcaa tcagcgccag cagggcgata atgatcagcg ccaggcgtac cgaccagcgg
     3661 gaagagagga aaaagttcac gcgtgcgctc cttcatgacg acgaatgcgc gggtccagcg
     3721 cggcgttgag caaatcgaca atcaaattac agaccacaaa aaccaccacc atcatcagcg
     3781 taaagcactg gatcaccgga tagtcacggt taaaaatcgc cgacaccgca tagcgcccga
     3841 cgcccggcca ggcaaagatg ttttcgataa tcatcgtccc gccaatcagt tcgccgatat
     3901 gcatccccac ggcggtgatc atcggcagcg aggcattgcg caggatgtgg cgacgttcgg
     3961 tctgtttgtc gttcaggccg cgcagacgcg cccaggtgac gtgacgctga ccggcgacgt
     4021 ccagcatact ggcgcgcagt aaacgcgcgt taatcgccag cgacataaag gcaatggaaa
     4081 ccgcaggcaa aatgatgtgc tgccagccgc cgtaacccat cgcgggtagc cattgcagat
     4141 acaccgaaaa cgccatcacc agtaaaaacg ccagccagaa gttaggcatc gacacgccaa
     4201 gaaacgcgat gaaacgtacg gcgaaatccg gcagacggtc gcgatggcgc gccgcccaga
     4261 taccgagcgg tacggaagtg agcagaatta ataccagcgc cgcacctgca agttccagcg
     4321 tggcgggcag gaagttcagc atatcgtcca gtaccgggcg ttggctggcg aatgagatac
     4381 caaagtcaag atgcagcgcc ttccacaacc aggtaccgta ctggacgtac agcggctgat
     4441 ccagcccgag catggtgcgg gtagaggcca gcatctccgg cgtcggcggc aggttagata
     4501 aacgcagata atcgagcgcc gggtcgccgg taccgaggcg cagcatcaga aaaatgatca
     4561 ccgaggcggc gagcaccatc gggatcagca gcagaaagcg gcgtaatacg taacgcaaca
     4621 ttaaggtttc accggtttaa tctgttcgaa cggaatttcg gtggcgatcg gcgcgtaggg
     4681 gatgttaccc agctccggtt ttgataccac catcattgag atgtaactga taggcagata
     4741 aaccgcctcg tcatgcagac gggtcagaat gtcgcgatac agcgcctgac gttgcgtttc
     4801 gtcatgggtc gccagcactt cgccgatctc tttatcaatc agcggtttgt cggctaatcc
     4861 ttgctgtgcc tggaagtcag cgtgtgacgg tacgcgcatt gaactgagga aggcgtgtgg
     4921 atcatatggc gcgccccagg tgcggtggaa aatcatgcca aaacgaccgt cgcgctgacg
     4981 agcatagata ctgctctctt cttcgccaat cagcgagaca tctgcgccaa tctggcgcat
     5041 atcagcctga atgatttccg ccatcgattt gcttaacgca tcggtgccga tgaacgacag
     5101 ttcaatgcgc agcggctgac catttttctc gcggatgtct ttgcccgcag gcagcgtcca
     5161 accggctttt tccagcaacg ctttcgcttt ctgcggatcg tactggcgcg gtttcaggcc
     5221 gaggttggcg tagggcacag aaggggcaaa cagggtgtcg gcgacctgct gggtgccata
     5281 caacgcgtta tcaatcagcg attttttgtt taccgcgtaa ttaagagctt cacgtactgc
     5341 cagctcgttg gtgggggctt tggcggtatt gagcgccagc atcacggttt cgatcggctg
     5401 tgacagttgg gtgtggtaag ccggattctg gctaaagcgg gcgaaggtat cgagcggtaa
     5461 taacccttcg tttccgtaca gcaggtcgat atcgccagtt tcaaacgcca ccgcgcgggt
     5521 agtcgggtcc gggatgacgt taaaggtgat ctttttaatc gctggctttt cgccccagta
     5581 gttttcgtta cggacgaaga catcgtactg attcagtttc gattcctgca aaatccacgg
     5641 tccggtgcca atcggcgctt taattccgtt catggtttca tggtttttaa actgcgaggg
     5701 agcgataaaa cggaaaggac ggggcagggc cagttcttgc aggaaaggat agtaggcgct
     5761 tttcagggta atttgcagct ctgttttact gagtgcttta acatcaacaa tctggtttgc
     5821 cagctccagc caggcgtgac gttgacggtt atcgagcact gcgcggaagt tttctgccgc
     5881 cgcctcggca tcgaacggtt caccgttgga gaatttcacg tcatcacgca gggtgaaggt
     5941 ccaggtttta ccatcttctg aatgagtcca gctttttgcc agccacggga tcaccgaacc
     6001 gtctgcctga tatttcacca atggttcata aaccatgctc tgggcgaaca tctggttagg
     6061 cgtgtaaagg tgcgggttta gtggcccgac attcaccggc caggcggtgg tgatttcatc
     6121 tggtgcagcg gcatggacga taaaagacgc acaagccagc agcgcaaata gagtgcggcg
     6181 gagtgtggag agcatggtaa ccccaatgga ttaaaataga tggcggaaat aagtatgacg
     6241 attttaagta ttcgtcatac tgattacctg ttctggatca atagatgggc agtcgggtgg
     6301 gcggatcagt taactgaatc gatccattgc acactgtcgg cggtgagcgt aaagggagta
     6361 ggggtgcaga tcgccaggct taaattttca agctgacaat ggctgactga cagcgaggag
     6421 tgataggtgc tgtctacgct gacgatttgc caggcgctgc cgccacgctg tttaacgatg
     6481 gcttctttgc gcgtccagat gcgccagaac atttccagtt gctgatcagg atgcacggcg
     6541 tccatctcag cgtgttcccc gaggctgaat acggcgttcg ccagccagtg ccagttggcg
     6601 cgcgggcgaa tcacttcgat atcgcagccg acttcgcctt catcactcaa cagcagggcg
     6661 atatcgtcac cgctatggct taagttgaac catagcggca tttccggcgc aaatgcaggt
     6721 ttgccttgtt cgccatagat gatctccggt agcggggaaa gcgtgtgcga aagcaatgca
     6781 cgccccgcca gccagcgttc gcgtcgtgga ccttgcggtg cttgctcgcg taaacccggt
     6841 ggcagtggag ctgcgcttaa ggtcgaaact ttccccagaa ctatccgata catatcaggg
     6901 ccaacgttta atggaaaatg aaagtgcgta tcgtatcact tgtcgcctca tcccggtaac
     6961 cgacttttcg gtctgcctgg ccccagtaaa atcgccagtt tgctaccgcc tttggtggtt
     7021 tccatccaga ttttacacac actggttaat ggcaccgaaa gtagcatccc taccgggccg
     7081 agcagccatc cccaaattaa caatgaaaga aataccacca tggtggacat ccccaggcga
     7141 tggcccatca tccgtggttc taaaatattg ccgatgacca tatggaccac taaaaacaat
     7201 gcgccgacca gaatacattc gtaaacacca ttaaacagca gcacctgaat cattggcggt
     7261 acggcggaaa ttaccgcgcc gatattgggc acgtagttga gcaaaaacgc cagtactgcc
     7321 cacatcagcg caaactgcac ccccatcagc tccagcccca gccagacgat gacacctgtc
     7381 cataaactga gtagcgtctt caatgcaaga tagtgcgaaa cgcctttaag tgcgcggtgt
     7441 aatcctgcga tgtgaatctg tgggttattc agcgcaaaac gcattttgta agggacgtgg
     7501 cgcacttcaa acagcataaa aactacggtc atcaccagca aaagcacgct cgccattgcc
     7561 ccggaaagcc cggtcattag cgctgtggtg aaggtaacca ctttttccga gtccatccgc
     7621 tgcagcattc gctccggcga catatgcaaa ttaagaaaag gcaacatctc ctgcaattta
     7681 aaaagtttgc gcgtcaactc cttattaaac ttcggcagca tagagataaa ttcgttaaac
     7741 gatgccgcca gtacgccgac cagcgcagtt agtgcgatca gcatcaccac caccacaatc
     7801 gtaatggcaa tggggcgttg tactccccga cgaataaacc aggtgacgag cgggttgagg
     7861 acgatggcaa aaaacagcgc cagtaacagc tgcacaatga tatctgccgc tgcgtgaatg
     7921 cccgcgagga tcactaccag cgaggccagc ttgagcagaa tgtgcatgcc cgttttatcg
     7981 ggttgagggg tttccattgg ggcttccttg tgactttttg tattaagtgt agcgggagtc
     8041 acgccagcaa tattctgatt cttactgctg attttcacgt cagcacgtgg taaaaatgaa
     8101 acactgttgt aaaaatgtgg tgatcctcat gcccgaaccc gtagccgaac ccgcgctaaa
     8161 cggattgcgc ctgaatttgc gcattgtctc cattgtcatg tttaacttcg ccagctacct
     8221 caccatcggg ttgccgctcg ctgtattacc gggctatgtc catgatgtga tgggctttag
     8281 cgctttctgg gcaggattgg ttatcagcct gcaatatttc gccaccttgc tgagccgtcc
     8341 tcatgccgga cgttacgccg atttgctggg ccccaaaaag attgtcgtct tcggtttatg
     8401 cggctgcttt ttgagcggtc tgggatatct gacggcagga ttaaccgcca gtctgcccgt
     8461 catcagcctg ttattacttt gcctgggacg cgtgatcctt gggattgggc aaagttttgc
     8521 cggaacggga tcgaccctgt ggggcgttgg cgtggttggc tcgctgcata tcgggcgagt
     8581 gatttcgtgg aacggcattg tcacttacgg ggcgatggcg atgggtgcgc cgttaggcgt
     8641 cgtgttttat cactggggcg gcttgcaggc gttagcgtta atcattatgg gcgtggcgct
     8701 ggtggccatt ttgttggcga tcccgcgtcc gacggtaaaa gccagtaaag gcaaaccgct
     8761 gccgtttcgc gcggtgcttg ggcgcgtctg gctgtacggt atggcgctgg cactggcttc
     8821 cgccggattt ggcgtcatcg ccacctttat cacgctgttt tatgacgcta aaggttggga
     8881 cggtgcggct ttcgcgctga cgctgtttag ctgtgcgttt gtcggtacgc gtttgttatt
     8941 ccctaacggc attaaccgta tcggtggctt aaacgtagcg atgatttgct ttagcgttga
     9001 gataatcggc ctgctactgg ttggcgtggc gactatgccg tggatggcga aaatcggcgt
     9061 cttactggcg ggggccgggt tttcgctggt gttcccggca ttgggtgtag tggcggtaaa
     9121 agcggttccg cagcaaaatc agggggcggc gctggcaact tacaccgtat ttatggattt
     9181 atcgcttggc gtgactggac cactggctgg gctggtgatg agctgggcgg gcgtaccggt
     9241 gatttatctg gcggcggcgg gactggtcac aatcgcgtta ttactgacgt ggcgattaaa
     9301 aaaacggcct ccggaacacg tccctgaggc cgcctcatca tcttaaaact tactgaataa
     9361 ccagcgtatt aatgatgttt tctgcggtgg tctgcgcttt ttgctgatcg tcagctggca
     9421 gagtaatttg catggtcagc agttgattac ccacgttacc cagaataacg gaagagtacg
     9481 ccgtctggcc tttcgcggag ataatactgt ctaactgctg cattttgtga cctttcagct
     9541 caatggcttt attggttacc acttgcagct gcggatcgcg gctacgttgc tgatcttcca
     9601 gacgcttcgc cagcaccgcc agatcttctt ttggatcatc gcccatgatg acgatgactg
     9661 ctttctgccc ggtggcgtcg gaccagacat gcatgttatt ggcctgcgtt cccagcttac
     9721 cgctctggtc ggtcatatcc gctggcagcg agaaacttaa cttgccatca agcaggttga
     9781 cgggattccc ggtagcgtta ctttccgcga ccgaaccctg cgccgtagcg ttagtgtctt
     9841 tatcatcaca ggccgcaagc cccataacca gcaggccaat tccgacatat ttaaccagat
     9901 tgcgcattga cttcttcctt tcgataaacg gccataacgg ctcattcatc catcttatca
     9961 caactctgat aacgaacctt taactcgcct gcaaagcgtt gatttcggat ttatctgcca
    10021 gtcttttcaa cagcatattg agtaatacgc catacattgg caggaagaaa acgatactga
    10081 ttaacacttt gaaacagtaa tcgaccagcg cgatttccat ccagtgttca gccataaagg
    10141 catccgggct acgccagaag gcaatgaaga aaaaggccag cgtgtcgctg acgttaccga
    10201 acagtgtgga cgctgtcggt gccagccacc agcggcgact ttgacgcagg cggttaaaaa
    10261 cgtgcacgtc gaggatttgc cccagcgcgt aggccatgaa actggcggtg gcgatacggg
    10321 cgacaaacag gttgaagtgg gcgagtgcgc cgaatccctg ccatgaaccc atatagaaca
    10381 gcgacgagat gacataggag attaataacg cagggatcat taccgcgaag ataatgcgtc
    10441 gggccagcgg tgcgccaaaa atacgcacgg tcaggtcggt agcaagaaaa ataaacggaa
    10501 agctaaacgc gccccaggtg gtatggaaac caaaaatgga gacgggaagc tgaaccagat
    10561 agttactgga ggtgatcacc agcagatgaa ataacgataa ccagaacaac gccttatagc
    10621 gttgagtttg cgagaaaacg ttcatattgt acctttttga ttaaccattg gggtgaggga
    10681 acccaatacg tacgacacgt ctgttacctt aacataataa cgtgcttacc cttttttcga
    10741 gccgccgcat gatactgctt tacgttgaca atgcaatggt taattttcac gcaatcgtta
    10801 acctggtttg cttacggact cacagggcgt aaactagcgc cgttttttta tgtgatgaga
    10861 agaaaatgac cgatctcttt tccagccctg accacacact cgacgcactt ggcctgcgct
    10921 gcccggaacc cgtaatgatg gtgcgcaaaa ctgtgcgcaa tatgcagccc ggcgaaacgt
    10981 tgctgattat cgccgacgat ccggccacta cccgcgatat tcctgggttt tgtaccttta
    11041 tggaacacga actggttgcg aaagagacgg atggactgcc ttatcgttat ttgattcgta
    11101 aaggcggttg atgggggctg attggcttcg atgccgcctt ttcccctcac cctaaccctc
    11161 tccccagagg ggcgagggga ccgatcgcgc tcaatgttgc gatcggtttg ccttatctcc
    11221 tgcgcaacaa tcttaacgca ttcgctgtca ccagcaccgt cgcacccgta tctgccagca
    11281 ccgccagcca tagtccggtc atccctaaca gcgtggtgac gaggaagatc cctttcagtc
    11341 ccagcgcaat ggtgatgttc tggcggatat tggcgtgagt ggcgcgtgcc agttcaatca
    11401 tttgcaccag gccgcgcagg tggttatggg ttaatgctgc atcggcggtt tccagcgcca
    11461 cgtctgtgcc gctgcccatt gcaatcccaa tggcggcagc tttcatcgct ggcgcgtcgt
    11521 taataccatc accgaccatc gccagcggcg catgttgatt cagcttggtc accgctttga
    11581 ctttctcttc cggcaatagg cccgctttaa actccagccc cagctccccg gcgattgccg
    11641 ccgctgcgcg tggattatcg ccggtgagga tcactccttt aactcccagc gcgttcagtt
    11701 cactgatggc agttgcagcg tcggcgcgca gggtatcctg caatgcaatg atacccagca
    11761 cgtcatcatt acgtactacc agcacaaccg tttgcccggc gctttccagt tcattaatca
    11821 aaccagcaaa tgcatcagcg ggatgtttcc cggcagcgca aatcaatacg cgctcaccgt
    11881 taacctgcgc ttcaatgcca gacccgacca gcgcccgctg tgattcggcg gtgggaatgg
    11941 cgagttcagc aacctgtgct tctcgcacga tggcctgcgc cagtggatgc gtcgcacctt
    12001 gctcgaccgc cgctgccagt gtcagcagtt cagattcact aatacccgtt gccggatgaa
    12061 tcgcggtaac gcgcggttta ccgacggtca gcgtaccggt tttatcaaac gccacctggg
    12121 taacacgacc tagttgttcc agcgccgctc cgcctttaat taacgcccca cgacgcgctg
    12181 ctgccgccag cccggaggta atcgccgcgg gcgtggagat aactaacgca cacgggcagc
    12241 caatcagcag cagcgtcagg cctttataaa tccactcttg ccagctggcg gcaaacagca
    12301 gcggtggcac cagcgtcacc agcagagcga cggccataat cgcgggcgta tagatacggc
    12361 tgaaacggtc gataaaccgc tcaatgggtg cgcgacgctc ttcggcttct tcgatcagtt
    12421 tcagaatccg gtcaatggcg ctggcacccg gttctgacag cacttccagc gttaccagac
    12481 ggtctacgct ggtggctcca gcagggactt tatcgcccgt tgcgcgctcc accggaatag
    12541 attcgccggt cagggcgctt tcatcaaaac tggcaaacgg tgagagcagt ttaccgtcgg
    12601 caggcaaacg tccacctgcg gcgacttcaa tcacatcgcc agggcgcagg ctgttaatcg
    12661 ccacctcttc ccgctcaccg ttacgcaggc gcgtggcggt ttctggtttc agcgccatta
    12721 acgcgctgac cccttgacgt gcgcggctgg cggcccagcc ttccagtcgt tcgccaatca
    12781 aaaacagcag caacaccatc gcagcttcag ccgttgcgcc aataaaaagc gcaccaatag
    12841 cggctacgct cattaaggtt tcaatggcga agtagctgcc ggatttgatc aaccgtaatg
    12901 cctgacgagc aatcgggtac agcccaacca gtgtggtcgc gataaacgcc agttgcccga
    12961 acggatgatt gaactgctcc agaccccagc tgattgccat catcacgatt agcgtaatca
    13021 gcggcagatt ctctttcagg cgtgatgctt gcggttcgtc ggcggactgt tcatcgcgca
    13081 gggagtagcc cgctttttgc accgcagatt caacctgggc acggatgtca ttgtcggcat
    13141 caaccaccag tttttcggtg gcgaacaaca cctgtacctg attcacgcct gcaagctggc
    13201 gcacggcatt ttctaccttg cgcgcacagg cggcgcagtc catgccgctg actttccagc
    13261 tatagcgggt gccggagacg ttttcagaga gagttggcgt gctggaacat gcgccgtcgc
    13321 agcaacagtc gttggcgttc tgtaccgtgg ttagcggttt gaacgcagca aattgagggg
    13381 ctttcttgcc gtgattgtca ggagtcgaca tggcatcctc cggttaagtt ttttctcatt
    13441 aaccgaagga tacactctgg agtcgactcc agagtcaagt tttatcagag atacagcgag
    13501 cggacgatca ggaagtgacc ggcaaagtag caggccgcag caatcgcgtt atccgcacgg
    13561 aagcgtcggc gatagtgact acccagccag acaaagttac tgataaacag caacgaagcg
    13621 ccgacaaacg cagagagcgc cggagcggtc ggacggaaga accacagttc acctgccagc
    13681 cacaccatca ccagcgtcat gccgataaac gtgcagatag gccaacgata ctcttccagc
    13741 cgcgtccaga taatcgccag taacagcgcg cccagaacca gcagcaccag tggcagaggc
    13801 cagaagaaag agagcgtcat ctgactggcg aaatagatgg tgtacagcag gtgcgagagg
    13861 aaaaacgcgc cgatggcgta catcagacgt tgacgtggca acagggttag cgcatcgccc
    13921 agcagtgagg cgcacagccc cgccagcacc agatagctaa tggcgtcgaa catcggcgct
    13981 tgccaggcca gtaacagcag gagaagaagg gttaatggtt taaataccca gcgttgccag
    14041 gttggcccac gatacgatgc atccacagat agccatgcgg aaagacagac agcgataaac
    14101 gaccaaagca tcttagttcc ttgaattcgt tattttttcg taagcggaat gcctacggtc
    14161 ttgcttccag tttagtggcg agggtggacg gatgacaaca ccataactgg cagggtatgc
    14221 ttaattccgc attttccgat gagggataaa gatgagcaaa ccacctcttt tctttattgt
    14281 tatcattggc ttaatcatcg tcgccgcatc gtttcgtttt atgcaacaac gacgggaaaa
    14341 agctgataat gatatggcac cactccagca aaagctggtg ttggtgagca acaagcggga
    14401 aaaaccgatt aacgatcgcc gttcgcgcca gcaggaagtg actccggcag gcaccagtat
    14461 gcgctatgag gcaagcttca aaccgcaaag cggaggaatg gagcagacgt ttcgcctcga
    14521 cgcccagcag taccacgccc tgacagtggg cgataaaggt acgctgagct ataaaggaac
    14581 gcgctttgtc agctttgtag gcgaacaata aggttatttt ttaactttaa atttcttctg
    14641 ccagaccagc agttcaaaca cgccgaaaag gaaaatccgt accttttcgg cggtggtcat
    14701 ctgcgggcca tctttcggta aagtggattt caatagcgcc agctgcatac catgcatcag
    14761 cacggtaaaa atcagcgcca cgttaacgaa aatattcagc ggacgtggga agggatgcac
    14821 caggttgagg attaaaaatc cccaaacaca gagcattaac aaacgaccaa tattaatcag
    14881 catcactttc tccttgtgct tcgcgttgat acagccgata agccacctga cccgccactt
    14941 tttcccgatg taatgaccag tttgccggaa cagtgggcag accgttttcg acttcgcttt
    15001 cgacataaat cagggcttcg tcagccagcc agccgttatc ttccagtaaa tttatcgtct
    15061 cttctaacaa gccacggcgg aacggtggat cgacaaacac gatattatgc ggtgtacctt
    15121 tttgcgccag gaatgacatc gcgttgctgt tcaccacgcg tgcattgcct gcttttagtg
    15181 tcgccagatt cttaattaac tgctgagaaa ccgcgcgatc catctcaatc aacgttgccc
    15241 ccgcagcata gcgcgacaac gcttccagcc ccagcgcgcc gcttccggcg aagcaatcca
    15301 gacattgggc gtcaacaatg accggagcca gccagttaaa caacgtttcg cgtacgcggt
    15361 cggtggtggg gcgcagacct gggctatcag gaaccgggag tttacggcct cgccactgcc
    15421 cgccaataat gcggatttgg ccgctgccgg aatgattcgg ttttttcatg ataaattgct
    15481 caatccgcca gataacacat acttgcaggc ggtgatgtga attaagttaa gtgatagact
    15541 atttcatcat ttttttagct gctatgtaca tagcgttaac gctgtgccat gaagcaacag
    15601 cgaggagtgt agtcgcaaat ggcgaaagaa aaaaaacgtg gctttttttc ctggctgggc
    15661 tttggtcaaa aagagcagac cccggaaaaa gagacagaag ttcagaatga acaaccggtt
    15721 gtagaagaaa tcgttcaggc gcaagagcct gtgaaggctt cggaacaagc cgttgaagag
    15781 cagccgcagg cgcatactga agccgaggcg gaaacttttg ctgccgacgt tgtggaagtc
    15841 actgaacagg ttgctgaaag tgaaaaagcg cagcctgaag cggaagtcgt tgcacagccg
    15901 gaaccggtcg cagaagaaac gccggagcca gtggctatcg aacgtgaaga gctgccgttg
    15961 ccagaagacg tcaacgccga agaggtttcg ccagaagagt ggcaggctga agcggaaacc
    16021 gtagagattg tcgaagcggc agaagaagaa gcggctaaag aagaaatcac cgacgaagag
    16081 ctggaagcac aggcgctggc tgccgaagcg gcagaagagg cggtgatggt agttcctccg
    16141 gcagaagaag agcagccggt ggaagaaatt gcccaggagc aagaaaaacc gaccaaagaa
    16201 ggtttcttcg cgcgcctgaa acgcagcctg ttaaaaacca aagaaaatct cggttccgga
    16261 tttatcagcc tgttccgcgg taaaaaaatc gacgatgatc tgtttgagga gctggaagaa
    16321 cagctgttga tcgccgatgt gggcgtggaa accacacgta aaattatcac caatctgacg
    16381 gaaggcgcat cccgcaagca gcttcgtgat gccgaggcgc tctatggcct gctgaaagaa
    16441 gagatgggcg agattctggc gaaagtcgat gagccgctga atgttgaagg taaaacgccg
    16501 ttcgtgatcc tgatggtggg cgtcaacggt gtgggtaaaa ctacgacgat tggtaagctg
    16561 gcacgtcagt ttgagcagca gggtaaatcg gtgatgctgg cggcgggcga tactttccgt
    16621 gcagcagcgg ttgaacagct tcaggtctgg ggtcagcgca acaatattcc ggtgattgcc
    16681 cagcataccg gtgcggattc cgcctctgtt atcttcgacg ccattcaggc agctaaagcg
    16741 cgtaatatcg acgtcctgat tgccgatacc gccggacgcc tgcagaacaa atcgcacctg
    16801 atggaagagt tgaagaaaat cgtccgcgtg atgaagaaac tcgacgttga agcgccgcat
    16861 gaagttatgc tgactattga tgccagtacc gggcagaatg cggtaagcca ggccaaactg
    16921 ttccacgaag ccgttggctt aaccggcatc acgctaacga aactggacgg cacggcgaaa
    16981 ggcggggtaa ttttctcggt ggctgaccag tttggtatcc ctatccgcta cattggtgtc
    17041 ggcgaacgta ttgaggattt gcgtccgttt aaggcggacg actttataga ggcacttttt
    17101 gcccgagagg attaacaatg attcgctttg aacatgtcag caaggcttat ctcggtggga
    17161 gacaggcgct gcagggcgtt acgttccata tgcagccggg tgagatggcg tttctgaccg
    17221 gtcattccgg cgcagggaaa agtaccctcc tgaagctgat ctgtgggatt gagcggccca
    17281 gcgccgggaa aatctggttt agcggccatg acatcacgcg tctgaaaaac cgtgaagttc
    17341 cgtttctgcg ccgccagatt ggcatgattt tccaggatca ccatctactg atggaccgta
    17401 ctgtctacga taacgtggcg atcccgctga ttatcgctgg tgccagcggt gacgatattc
    17461 gtcgccgggt gtcggcggca ctggataaag tcgggctact ggacaaagcg aagaacttcc
    17521 ctattcagct ttccggcggt gaacaacagc gtgttggcat tgcccgcgcg gtggtgaata
    17581 agcccgcggt actgctggcg gacgaaccga ccggtaacct ggacgacgcg ttgtcggaag
    17641 ggattctacg tctgtttgaa gagtttaacc gcgttggggt aaccgtattg atggcaacgc
    17701 acgacatcaa cctgatttca cggcgttcct atcgcatgct caccctgagc gatggtcact
    17761 tgcatggagg cgtgggccat gaataagcgc gatgcaatca atcatattcg gcagtttggc
    17821 gggcgtcttg atcgcttccg taaatcggtc ggcggctctg gcgacggcgg tcgtaacgcc
    17881 ccgaaacgcg cgaaatcctc gccaaaaccg gtaaatcgta aaaccaacgt tttcaacgaa
    17941 caggtgcgct atgccttcca cggcgcattg caggatctga aaagcaaacc gttcgccacg
    18001 tttttaacgg tgatggttat cgccatctcc ctgacgctgc ccagcgtctg ttatatggtg
    18061 tacaaaaacg ttaatcaggc ggcgacgcag tattatccgt caccgcaaat cactgtttac
    18121 ttgcaaaaaa cgctggacga tgacgccgct gcgggcgtgg tggcacagtt gcaggccgag
    18181 caaggcgtgg agaaagtgaa ctatctttct cgtgaagacg cactgggcga gttccgtaac
    18241 tggtctggtt ttggtggtgc gctggatatg ctggaagaaa acccgcttcc ggcagtggcg
    18301 gtggtgatcc cgaaactcga ttttcagggg acggaatcac tcaatacgct gcgtgatcgt
    18361 atcacgcaga ttaacggcat tgacgaagtg cggatggatg acagctggtt tgcccgtctg
    18421 gcggcgttga ccgggctggt cgggcgcgtt tcggcgatga tcggcgtgtt gatggtggcg
    18481 gcagtgttcc tcgtcatcgg caacagcgta cgtctgagca tctttgctcg ccgtgactcc
    18541 atcaacgtgc agaaactgat tggtgcgacg gatggtttca tccttcgccc gttcctgtat
    18601 ggtggcgcac tgctgggatt ttctggcgca ttgttgtcat taattttgtc agaaattctg
    18661 atgctgcgat tgtcatcggc ggttgcggaa gtggcacagg ttttcggaac gaagtttgat
    18721 atcaatggtt tatcgttcga tgaatgcctg ctattgctgc tggtatgctc gatgattggc
    18781 tgggtggcag cgtggcttgc cacggtacaa catttacgcc actttacgcc tgaataataa
    18841 aagcgtgtta tactctttcc ctgcaatggg ttccgtagca gggaaagaga tcccgttgtc
    18901 tcttcccggt atttcatctc tatgtcacat tttgtgcgta atttattcac aagcttgcat
    18961 tgaacttgtg gataaaatca cggtctgata aaacagtgaa tgataacctc gttgctctta
    19021 agctctggca cagttgttgc taccactgaa gcgccagaag atatcgattg agaggatttg
    19081 aatgactgac aaaatgcaaa gtttagcttt agccccagtt ggcaacctgg attcctacat
    19141 ccgggcagct aacgcgtggc cgatgttgtc ggctgacgag gagcgggcgc tggctgaaaa
    19201 gctgcattac catggcgatc tggaagcagc taaaacgctg atcctgtctc acctgcggtt
    19261 tgttgttcat attgctcgta attatgcggg ctatggcctg ccacaggcgg atttgattca
    19321 ggaaggtaac atcggcctga tgaaagcagt gcgccgtttc aacccggaag tgggtgtgcg
    19381 cctggtctcc ttcgccgttc actggatcaa agcagagatc cacgaatacg ttctgcgtaa
    19441 ctggcgtatc gtcaaagttg cgaccaccaa agcgcagcgc aaactgttct tcaacctgcg
    19501 taaaaccaag cagcgtctgg gctggtttaa ccaggatgaa gtcgaaatgg tggcccgtga
    19561 actgggcgta accagcaaag acgttcgtga gatggaatca cgtatggcgg cacaggacat
    19621 gacctttgac ctgtcttccg acgacgattc cgacagccag ccgatggcac cggtgctcta
    19681 tctgcaggat aaatcatcta actttgccga cggcatcgaa gatgataact gggaagagca
    19741 ggcggcaaac cgtctgaccg acgcgatgca gggtctggac gaacgcagcc aggacatcat
    19801 ccgtgcgcgc tggctggacg aagacaacaa gtccacgttg caggaactgg ctgaccgtta
    19861 cggtgtttcc gctgaacgtg tgcgccaact ggaaaagaac gcgatgaaaa aacttcgcgc
    19921 cgctattgaa gcgtaatttc cgctattaag cagagaaccc tggatgagag tccggggttt
    19981 ttgttttttg ggcctctaca ataatcaatt ccccctccgg caaaacgtca atccccacgc
    20041 agattgttaa taaactgtca aaatatctat tccaatatca taaaaatggg gtatgtttta
    20101 gcagagtatg ctgctaaagc acgggtagtc atgcataaaa cgaaataaag tgctgaatca
    20161 caacaccaca acacacgtaa taaccagaag aatggggatt ctcaggatga atataaaggg
    20221 taaagcgtta ctggcaggat gtatcgcgct ggcattcagc aatatggctc tggcagaaga
    20281 tattaaagtc gcggtcgtgg gcgcaatgtc cggtccggtt gcgcagtacg gtgaccagga
    20341 gtttaccggc gcagagcagg cggttgcgga tatcaacgct aaaggcggca ttaaaggcaa
    20401 caaactgcaa atcgtaaaat atgacgatgc ctgtgacccg aaacaggcgg ttgcggtggc
    20461 gaacaaagtc gttaacgacg gcattaaata tgtgattggt cacctctgtt cttcatcaac
    20521 gcagcctgcg tctgatatct acgaagacga aggcatttta atgatcaccc cagcggcaac
    20581 cgcgccggag ctgaccgccc gtggctatca gctgatcctg cgcaccaccg gcctggactc
    20641 cgaccagggg ccgacggcgg cgaaatatat tcttgagaaa gtgaaaccgc agcgtattgc
    20701 tatcgttcac gacaaacagc aatacggcga aggtctggca cgagcggtgc aggacggcct
    20761 gaagaaaggc aatgctaacg tggtgttctt tgatggtatc accgccgggg aaaaagattt
    20821 ctctacgctg gtggcgcgtc tgaagaaaga gaatatcgac ttcgtttact acggcggtta
    20881 ccacccggaa atggggcaaa tcctgcgtca ggcacgcgcg gcagggctga aaactcagtt
    20941 tatggggccg gaaggtgtgg ctaacgtttc gctgtctaac attgcgggcg aatcagcgga
    21001 agggctgctg gtgaccaagc cgaagaacta cgatcaggtt ccggcaaaca aacccattgt
    21061 tgacgcgatc aaagcgaaaa aacaggaccc aagcggcgca ttcgtttgga ccacctacgc
    21121 cgcgctgcaa tctttgcagg cgggcctgaa tcagtctgac gatccggctg agatcgccaa
    21181 atacctgaaa gcgaactccg tggataccgt aatgggaccg ctgacgtggg atgagaaagg
    21241 cgatctgaaa ggctttgagt tcggcgtatt tgactggcac gccaacggca cggcgaccga
    21301 tgcgaagtaa tcattaatcg gcaactttgg gttgccgcca aattgctaat atcgagtacg
    21361 ttgcttcatg ccggatgcgg cgtaaacgcc ttatccggcc tacaagatcc aaagaaatca
    21421 gtaaattgca acacacattg taggcctgat aagcgtagcg catcaggcaa tttctcgttg
    21481 gtcatcactt ttgaaatcaa cttgacgatt aacgcttctc ccagccgccc tgttgcgccg
    21541 taaaccccag cgcctgcata aacgccgtca tcacaccgcg atcttccaag cctgcatccg
    21601 ccatccacca gcatgaaacg cctggattgt tacgcaaaac ctcttccagc agatattgcc
    21661 ccacaccgcg acgtcgggtg acttcccgca cacgcaggga atccagtgct ccctcggtgc
    21721 cgcttaaggt tacccgtacg gcagcgagca ggcgctcgtt aaaacgcgcg gcgtagatac
    21781 ggtggttatc gtcaacctgt aaagaggaag gggaatactc cggccagatc ttttgcaggt
    21841 caatccggtc ctggtcgcta aagttttcta atcgaatgat ggtcagcttc atgggtaacc
    21901 cgtgtaaatc acaaaagtgg aaccagtgta gcgaaataat ttaatcggag gctttctctt
    21961 ttttatttct tttggcaggt gattaatttt ttaacagcaa taattacaaa gttaaaacat
    22021 tagagaatga aaaataccca gcataatccc ctgaatggta gtgaattatt ccgccctttg
    22081 tgccgttatt ttatgctgac aaaggcactt ttttctgttt atctatcaat aaattcagaa
    22141 tattatctgt tcttaatcga ctgaaaaata gggattttaa tcactattat cacaaaatac
    22201 tgcgctaaac ccttaatcag acaggcaaaa acagtgcagt ataaaaaaag aacagtctga
    22261 tttgttaaca cataaaaaca aagcaacaca acatcacgaa tggggatttt tgactatgaa
    22321 acggaatgcg aaaactatca tcgcagggat gattgcactg gcaatttcac acaccgctat
    22381 ggctgacgat attaaagtcg ccgttgtcgg cgcgatgtcc ggcccgattg cccagtgggg
    22441 cgatatggaa tttaacggcg cgcgtcaggc gattaaagac attaatgcca aagggggaat
    22501 taagggcgac aagctggttg gcgtggaata tgacgacgcc tgcgacccga aacaagccgt
    22561 tgcggtcgcc aacaaaatcg ttaatgacgg cattaaatac gttattggtc atctgtgttc
    22621 ttcttctacc cagcctgcgt cagatatcta tgaagacgaa ggtattctga tgatctcgcc
    22681 gggagcgacc aacccggagc tgacccaacg cggttatcaa cacattatgc gtactgccgg
    22741 gctggactct tcccaggggc caacggcggc aaaatacatt cttgagacgg tgaagcccca
    22801 gcgcatcgcc atcattcacg acaaacaaca atatggcgaa gggctggcac gttcggtgca
    22861 ggacgggctg aaagctgcta acgccaatgt tgttttcttc gacggcatta ccgctggcga
    22921 gaaagatttc tccgcgctga tcgcccgcct gaaaaaagaa aacatcgact tcgtttacta
    22981 cggcggttac tacccggaaa tggggcagat gctgcgccag gcccgttccg ttggcctgaa
    23041 aacccagttt atggggccgg aaggtgtggg taatgcgtcg ctgtcgaata ttgctggcga
    23101 tgctgccgaa ggcatgttgg tcaccatgcc aaaacgctat gaccaggatc cggcaaacca
    23161 gggcatcgtt gatgcgctga aagcagacaa gaaagatccg tccgggcctt atgtctggat
    23221 cacttacgcg gcggtgcaat ctctggcaac agcccttgag cgtaccggca gcgatgagcc
    23281 gctggcgctg gtgaaagatt taaaagctaa cggtgcaaac accgtgattg ggccgctgaa
    23341 ctgggatgaa aaaggcgatc ttaagggatt tgattttggt gtcttccagt ggcacgccga
    23401 cggttcatcc acggcagcca agtgatcatc ccaccgcccg taaaatgcgg gcgggtttag
    23461 aaaggttacc ttatgtctga gcagtttttg tatttcttgc agcagatgtt taacggcgtc
    23521 acgctgggca gtacctacgc gctgatagcc atcggctaca ccatggttta cggcattatc
    23581 ggcatgataa acttcgccca cggcgaggtt tatatgattg gcagctacgt ctcatttatg
    23641 atcatcgccg cgctgatgat gatgggcatt gataccggct ggctgctggt ggcggcggga
    23701 ttcgtcggcg caatcgtcat tgccagcgcc tacggctgga gtatcgaacg ggtggcctat
    23761 cgcccggtgc gtaactctaa gcgcctgatt gcgctcatct cggcaatcgg tatgtccatc
    23821 ttcctgcaaa actacgtcag cctgaccgaa ggttcgcgtg acgtggcgct gccgagcctg
    23881 tttaacggtc agtgggtggt gggccatagc gaaaacttct ctgcctctat tactaccatg
    23941 caggcggtga tctggattgt caccttcctc gccatgctgg cgctgacgat tttcattcgc
    24001 tattcccgta tggggcgcgc gtgtcgtgcc tgcgcggaag atctgaaaat ggcgagtctg
    24061 cttggcatta acaccgaccg ggtgattgcg ctgacctttg tgattggcgc ggcgatggcg
    24121 gcggtggcgg gtgtgctgct cggtcagttc tacggcgtaa ttaaccccta catcggcttt
    24181 atggccggga tgaaagcctt taccgctgcg gtgctcggtg ggattggcag cattccaggg
    24241 gcgatgattg gtgggctgat cctggggatt gcggaggcgc tctcttctgc ctatctgagt
    24301 acggaatata aggatgtggt ttcatttgcc ctgctgattc tggtgctgct ggtgatgccg
    24361 accggtattc tgggtcgccc ggaggtagag aaagtatgaa accgatgcat attgcaatgg
    24421 cgctgctctc tgccgcgatg ttttttgtcc tggcgggcgt ctttatgggc gtgcaactgg
    24481 agctggatgg caccaaactg gtggtcgaca cggcttcaga catccgttgg cagtgggtgt
    24541 ttatcggcac ggcggtggtc tttttcttcc agcttttgcg accggctttc cagaaagggc
    24601 taaaaagcgt ttccggaccg aagtttattc tgcccgcgat tgatggctcc acggtgaagc
    24661 agaaactatt cctcgtggcg ctgctggtgc ttgcggtggc gtggccgttt atggtgtcgc
    24721 gtgggacggt ggatatcgcc accctgacca tgatctacat tatcctcggc ctcgggctga
    24781 acgtggttgt tggtctttct ggtctgctgg tgctggggta cggcggcttt tacgccatcg
    24841 gcgcttacac ttttgcgctg ctcaatcact attacggcct gggcttctgg acctgtctgc
    24901 cgattgccgg gttaatggca gcggcggccg gcttcctgct cggcttcccg gtgcttcgct
    24961 tgcgcggcga ctatctggcg atcgttaccc tcggtttcgg cgaaattgtg cgcatattgc
    25021 tgctcaataa caccgaaatt accggcggcc cgaacggaat cagtcagatc ccgaaaccga
    25081 cactcttcgg actcgagttc agccgtaccg ctcgtgaagg cggctgggac acgttcagta
    25141 atttctttgg cctgaaatac gatccctccg atcgtgtcat cttcctctac ctggtggcgt
    25201 tgctgctggt ggtgctaagc ctgtttgtca ttaaccgcct gctgcggatg ccgctggggc
    25261 gtgcgtggga agcgttgcgt gaagacgaga tcgcctgccg ttcgctgggc ttaagcccgc
    25321 gtcgtatcaa gctgactgcc tttaccataa gtgccgcgtt tgccggtttt gccggaacgc
    25381 tgtttgctgc gcgccagggc tttgtcagcc cggaatcctt cacctttgct gaatcggcgt
    25441 ttgtactggc gatagtggtg ctcggcggta tgggatcgca gtttgcggtg attctggcgg
    25501 caattttgct ggtggtatcg cgcgaattga tgcgtgattt caacgaatac agcatgttaa
    25561 tgctcggtgg tttgatggtg ctgatgatga tctggcgtcc gcagggcttg ctgcccatga
    25621 cgcgcccgca actgaagctg aaaaacggcg cagcgaaagg agagcaggca tgagtcagcc
    25681 attattatct gttaacggtc tgatgatgcg cttcggcggc ttgctggcgg tgaacaacgt
    25741 caatcttgaa ctgtacccgc aggagatcgt ctcgttaatc ggccctaacg gtgccggaaa
    25801 aaccacggtt tttaactgcc tgaccggatt ctacaaaccc accggcggca ctattttact
    25861 gcgtgatcag catctggaag gtttaccggg gcagcaaatt gcccgcatgg gcgtggtgcg
    25921 taccttccag catgtgcgtc tgttccgtga aatgacggtg attgaaaacc tgctggtggc
    25981 gcagcatcaa caactgaaaa ccgggctgtt ctctggcctg ttgaaaacgc cttctttccg
    26041 tcgcgcccag agcgaagcgc tcgaccgcgc cgccacctgg cttgagcgca ttggtttgct
    26101 ggaacacgcc aaccgtcagg cgagtaacct ggcctatggt gaccaacgcc gtctggagat
    26161 tgcccgctgc atggtgacgc agccggagat tttaatgctc gacgaaccgg cggcaggtct
    26221 taacccaaaa gaaaccaaag agctggatga gctgattgcc gaactgcgca atcatcacaa
    26281 caccactatc ttgttgattg aacacgatat gaagctggtg atggggattt cagaccgaat
    26341 ttatgtggtc aatcagggga cgccactggc aaacggtacg ccggagcaaa tccgtaataa
    26401 cccggacgtg atccgtgcct atttaggtga ggcataagat ggaaaaagtc atgttgtcct
    26461 ttgacaaagt cagcgcccac tacggcaaaa tccaggcgct gcatgaggtg agcctgcata
    26521 tcaatcaggg cgagattgtc accctcattg gcgcgaacgg ggcggggaaa accaccttgc
    26581 tcggcacgtt atgcggcgat ccgcgcgcta ccagcgggcg aattgtgttt gatgataaag
    26641 acattaccga ctggcagacg gcgaaaatca tgcgcgaagc ggtagcgatt gtcccggaag
    26701 ggcgtcgcgt cttctcgcgg atgacggtgg aagagaacct ggcgatgggc ggcttttttg
    26761 ccgaacgcga ccagttccag gagcgcataa agtgggtgta tgaactgttt ccacgtctgc
    26821 atgagcgccg tgttcagcgg gcaggcacca tgtccggcgg tgagcaacaa atgctggcga
    26881 ttggtcgcgc gctgatgagc aacccgcgtt tgctgctact tgatgagcca tcgctcggtc
    26941 ttgcgccgat tatcatccag caaattttcg acaccatcga gcagctgcgc gagcagggga
    27001 tgactatctt cctcgtcgag cagaacgcca accaggcgct aaagctggcg gatcgcggct
    27061 acgtgctgga aaacggccat gtggtgctct ccgataccgg cgacgcgctg ctggcgaacg
    27121 aagcagtgag aagtgcgtat ttaggcgggt aataacacgt tgattgataa ggagtcaaaa
    27181 gactcctttg agacaggtga caaatgtaaa attgcctgat gcgctgcgct tatcaggcct
    27241 actgggtgag ttgcaatata ttgaatttgc acgatcttgt aggccggata aggcgtttac
    27301 gccgcatccg gcatgaaacg atgggtaatc tgtagagttt gattcagacc ttctattttc
    27361 cgcttatccg tgccccatct cccattttcc ctcacccacg ccgtcaccgc cttgtcatct
    27421 ttctgacacc ttactatctt acaaatgtaa caaaaaagtt atttttctgt aattcgagca
    27481 tgtcatgtta ccccgcgagc ataaaacgcg tgaattcgcg cattcggtac aacaagagag
    27541 ataaacgatg aaaccgttac attatacagc ttcagcactg gcgctcggac tggcgttaat
    27601 ggggaatgca caggcagtga cgaccattcc gttctggcat tctatggaag gggaactggg
    27661 taaagaggtg gattctctgg cccaacgttt taacgccgaa aatccggatt acaaaattgt
    27721 accgacctat aaaggcaact acgaacagaa tttaagcgcg gggattgccg catttcgtac
    27781 cggcaacgct ccggctattt tgcaggttta tgaagttggc accgccacca tgatggcatc
    27841 gaaagccatt aaaccggtat atgacgtgtt taaagaggcg gggattcaat tcgatgagtc
    27901 gcagtttgtg ccgacggttt ccggttacta ctccgacagc aaaacgggcc acttactctc
    27961 ccagccattc aacagctcga cccccgttct ctattacaac aaagacgcct tcaagaaagc
    28021 aggattagac ccggaacaac cgccgaaaac ctggcaggat ctggcggact atgccgcgaa
    28081 actgaaagcc tccggtatga agtgcggcta cgccagcggc tggcagggct ggatccaact
    28141 ggaaaacttt agcgcctgga acggtctgcc gtttgccagc aaaaacaacg gctttgacgg
    28201 cacggacgcg gtgctggagt tcaacaagcc ggagcaggtg aaacatatcg ccatgctcga
    28261 ggagatgaac aagaagggcg acttcagcta cgtcggtcgt aaggatgaat ccaccgagaa
    28321 gttctataac ggtgattgcg cgatgaccac cgcctcttcc ggttctcttg ccaacattcg
    28381 cgagtacgcc aaatttaatt acggcgtagg catgatgcct tacgacgccg atgcgaaaga
    28441 tgcgccacaa aacgccatta tcggcggagc cagcctgtgg gtgatgcagg gtaaagataa
    28501 agaaacgtat accggtgtgg cgaagttcct cgatttcctc gcgaagccag aaaacgctgc
    28561 cgagtggcat cagaaaaccg gttatctgcc aattaccaaa gcggcgtatg acctgacccg
    28621 tgagcagggc ttttacgaga aaaacccagg tgcggatacc gcgacgcgtc agatgctgaa
    28681 taagccgccg ttgccgttca ccaaagggct gcgtctgggc aacatgccgc agatccgcgt
    28741 gattgtggat gaagagctgg agagcgtgtg gaccggtaag aagacaccac agcaggcact
    28801 ggataccgcc gttgagcgtg gaaatcagtt gctgcgccgc tttgagaaat cgacgaagtc
    28861 ttaatcagtg taatgtcgga tgcgtttcgc ttatctgact tggcatcgcg tgtaggccgg
    28921 ataagcgaag cgcatccggc acagttcagg aattaaccgt aatgtcatca tcccgtccgg
    28981 tgttccgctc gcgctggctg ccttatctgc tggtcgcgcc gcagctcatc atcaccgtta
    29041 tcttttttat ctggcctgcg ggcgaagcgt tgtggtactc gctacaaagc gtcgatccgt
    29101 ttggtttctc cagccagttt gtcggcctgg ataacttcgt cgcgctgttt catgacagct
    29161 actatctcga ctccttctgg acgacgataa aattcagcac ctttgtcacc gtcagcggtt
    29221 tgctggtgtc gctgttcttc gcggcgctgg tggagtacat cgtgcgcggc agccgtttct
    29281 atcaaacctt aatgctgctg ccttacgccg tggctcccgc cgttgccgcc gtattgtgga
    29341 tcttcctgtt taaccccggt cgcgggctga tcacccattt tctcgcggag ttcggctacg
    29401 actggaacca cgcgcaaaac agcggtcagg caatgtttct ggtggtgttt gcctcagtat
    29461 ggaagcaaat cagctacaac ttcctgttct tctatgccgc gctgcaatcc attccccgtt
    29521 cgttgatcga agccgcagcc atcgacggtg caggcccaat tcgccgcttc tttaagattg
    29581 cgctgccgct tatcgctccg gtgagtttct tcctgctggt ggtgaacctg gtgtatgcct
    29641 tcttcgacac cttcccggtg atcgacgccg ccacgtccgg cgggccggtt caggccacca
    29701 cgacgctgat ttataagatc taccgtgaag gctttaccgg actggatctg gcttcgtctg
    29761 ccgcgcagtc ggtggtgttg atgttcctcg tcatcgtgct gacggtggtg cagttccgct
    29821 atgttgaaag caaggtgcgt taccaatgat tgagaaccgt ccgtggctga cgatattcag
    29881 ccataccatg ctgatcctcg ggatcgcggt gatcctcttc ccgctgtacg tggcgtttgt
    29941 cgcggcgacg ctggataaac aggccgtcta tgccgcgccg atgacgctca tccccggcac
    30001 acatctgctg gaaaatatcc acaacatctg ggttaacggg gtaggcacga atagcgcgcc
    30061 gttctggcgg atgttgctta acagctttgt gatggcgttc agcattacgc tcggcaaaat
    30121 taccgtctcg atgctctcgg catttgccat tgtctggttt cgttttccgc tacgtaacct
    30181 cttcttctgg atgattttta tcaccctgat gctgccggtt gaagtacgta tcttcccgac
    30241 ggtggaagtc atcgccaacc tgaagatgct cgacagctac gccggtttaa cgctgccgct
    30301 gatggcctcg gcgaccgcca cgtttctgtt tcgccagttc tttatgacgc tgccggatga
    30361 actggtggaa gcggcgcgga tcgacggcgc gtcaccaatg cgtttctttt gcgacatcgt
    30421 ttttccgctc tccaaaacca atctggcggc gctgtttgtg atcaccttta tttacggctg
    30481 gaaccagtat ttgtggccgt tgttgattat taccgatgtg gatctcggca ccaccgtggc
    30541 agggatcaaa gggatgatcg ctacaggcga aggcaccact gaatggaact cagtgatggc
    30601 ggcgatgttg ttaacgctta tccctccggt ggtgattgtt ttagtgatgc agcgtgcctt
    30661 cgtgcgcggc ctggtcgata gtgagaaata agatggcagg actgaaatta caggcagtaa
    30721 ccaaaagctg ggatggtaaa acccaggtca ttaaaccgct gacccttgat gtggcggatg
    30781 gcgaatttat cgtgatggtc gggccgtctg gctgcgggaa atcgacgctg ctgcgcatgg
    30841 ttgccgggct ggagcgggtg acagaaggcg atatctggat caacgaccag cgcgtgactg
    30901 aaatggagcc aaaagatcgc gggattgcga tggtgttcca gaactacgcg ctttatccgc
    30961 atatgagtgt cgaagaaaac atggcgtggg ggctgaaaat tcgcggcatg ggcaagcagc
    31021 aaattgccga gcgcgttaaa gaagcggcgc gcattctgga gctggacggt ctgctcaaac
    31081 gtcgcccgcg cgagctttcc ggcggtcagc gccagcgtgt ggcgatgggc cgcgcgattg
    31141 tgcgcgatcc ggcggtgttc ctgtttgatg agccgctctc taacctcgat gccaagctgc
    31201 gcgtgcagat gcgtcttgaa ctgcaacagt tgcaccgtcg cctgaaaacg acttcactct
    31261 acgttactca cgatcaggtt gaagcgatga cgctcgccca gcgagtaatg gtgatgaacg
    31321 gcggcgttgc cgaacagatt ggcacaccag ttgaagtcta cgaaaagccc gccagcctgt
    31381 ttgtagcgag ttttatcggc agtccggcga tgaacctgct gacaggccgc gtgaataacg
    31441 aaggcacgca tttcgaactg gacggcggta ttgagctgcc gctaaacggt ggctaccgtc
    31501 agtatgccgg gcgtaaaatg actctcggca ttcgcccgga acatattgcg ctaagctcgc
    31561 aggcagaagg cggcgtaccg ctggtgatgg acacgctgga gatcctcggc gcagataacc
    31621 tggcgcacgg acgctggggc gagcagaagc tggtggtgcg actggcgcat caggagcgcc
    31681 cgacggcagg cagcacgctg tggctgcatc tggcggaaaa tcagctgcat ctttttgatg
    31741 gtgaaacagg acaacgagta tgagtaactg gccttatccc cgcatcgtcg ctcatcgtgg
    31801 cggcggtaag ctggccccgg aaaacaccct ggcggcaatc gacgtcgggg caaaatacgg
    31861 tcataagatg atcgaatttg acgcgaagtt atcgaaagat ggcgagatct tcctgctcca
    31921 tgacgacaat ctcgaacgca ccagcaacgg ctggggcgtc gcgggtgaac tgaactggca
    31981 ggatttactg cgcgtggatg cgggcagttg gtacagcaaa gcgtttaaag gtgagccgct
    32041 gccgttgctt tcgcaggtgg cggaacgctg tcgcgaacac gggatgatgg cgaatatcga
    32101 aatcaaaccc accactggca ctggaccatt aacgggcaaa atggtggcgc tggcggcacg
    32161 ccaactgtgg gccggtatga cgccgccgct gctgtcatcg tttgagattg atgctttaga
    32221 agctgcacag ctggcggcac cggaactgcc gcgtggttta ttgcttgatg agtggcgcga
    32281 cgactggcgc gaactgaccg cgcggctggg ctgcgtctct attcatctca atcataagtt
    32341 actcgataaa gtgcgagtga tgcagttgaa agacgccgga ctgtggattc tggtttatac
    32401 cgtcaataaa ccccagcgcg cggcagagtt gctgcgctgg ggcgtggatt gcatctgtac
    32461 cgatgcgatt gacgtgattg gtccgaactt tacggcccaa tagttttcaa cggaatgtca
    32521 ggctgcggcg tgctgggctg attcagcatg tcgccgtttc tcctctccgg cagcatatgc
    32581 tgctgattca acgaactatc cggattacgg ttgctgttta acatcccgcc gttggtgttg
    32641 ggcagcattt gctgccgggc gggatttcgc tcccccggct gtgactgcaa cacccgctga
    32701 gaattattgt ttatctggtt ttctaaatgc tgctgttgca actgagtttg cgttttcagt
    32761 tgctgattca gcatcccttt ttgctggatt tgctgagtct gcatctgggt ttgcatccgc
    32821 tgctggctgg gtatctgata ccccggctgg ttagggttgt tcagagtatt aatgggctgt
    32881 gcaaagccga caaacggcag gagtgccgta agaatcagaa gccgtttcat cgcgtatcct
    32941 cctctgaaga tatcctttaa gtttactcgc ttcccgacaa aacgatgatt aattcagagt
    33001 tatataccag gcttagctgg ggttgcccct taatctctgg agaataacga tgataaaacc
    33061 gacgttttta cgccgggtgg ccattgctgc tctgctctca ggaagttgtt ttagcgccgc
    33121 cgccgcgcct cctgcgccgc ccgtctcgta tggtgtggag gaagatgtct tccacccggt
    33181 acgcgcgaaa cagggaatgg tagcgtctgt ggacgccact gccactcagg tgggggtgga
    33241 tattctcaag gagggcggga atgccgttga tgccgccgtg gcggtgggct acgcgctggc
    33301 ggtaacgcat ccgcaggcag ggaatctggg cggtggtggt tttatgctga tccgctcgaa
    33361 aaatggcaat accaccgcta tcgatttccg cgaaatggca cccgctaaag cgacccgcga
    33421 tatgttcctt gatgaccagg gcaacccgga cagcaaaaaa tcactcactt cgcatctggc
    33481 ttccggcaca ccaggtacgg tcgctggttt ctcgctggcg ttggataagt acggcactat
    33541 gccgttgaac aaagtggtgc agcccgcgtt caaactggca cgcgatggtt ttatcgttaa
    33601 cgatgctttg gcagacgatc tcaaaaccta cggtagcgaa gtgttgccga atcacgaaaa
    33661 cagcaaagct atcttctgga aagagggcga accgctgaaa aagggcgaca cgctggtgca
    33721 ggcgaacctg gcaaagagcc tggagatgat tgctgaaaac ggcccggacg aattctataa
    33781 aggcacgatt gcggaacaga tcgcccagga gatgcagaaa aacggtggct tgatcactaa
    33841 agaagattta gcggcctata aagcggtcga acgcactccg ataagcggcg attatcgcgg
    33901 gtatcaggtt tactccatgc caccgccatc ctccggcggg atccatatcg tacaaatcct
    33961 caatattctg gaaaacttcg atatgaagaa atacggcttt ggcagcgccg atgcgatgca
    34021 aatcatggca gaagcggaga aatacgccta cgccgaccgc tcggaatatc ttggcgaccc
    34081 ggattttgtc aaagtcccgt ggcaggcact gaccaataaa gcctatgcca aatccattgc
    34141 cgatcaaatt gatatcaaca aagcgaagcc gtccagtgag attcgccctg gcaagcttgc
    34201 gccttatgag agtaatcaaa ctacccatta ctctgtagtg gataaagacg gtaacgcggt
    34261 ggcggtgacc tatacgctga acaccacctt cggtacgggt attgtcgcgg gcgagagcgg
    34321 tattctgctt aataaccaga tggatgattt ctccgccaaa ccgggcgtac cgaacgttta
    34381 cgggctggtg ggcggtgatg ccaacgccgt cgggccgaac aaacgcccgc tgtcgtcgat
    34441 gtcgccgacc attgtggtga aagacggtaa aacctggctg gttaccggta gcccaggcgg
    34501 tagccggatc atcactacag tgctgcaaat ggtggtgaat agcatcgatt atggcatgaa
    34561 cgtcgccgaa gcgaccaatg cgccgcgttt ccaccatcag tggttgccgg acgagctgcg
    34621 tgtcgaaaaa gggtttagcc cggatacgct caagctgctg gaagcaaaag gtcagaaagt
    34681 ggcgctgaaa gaggcgatgg gcagtacaca aagcattatg gttgggccgg acggtgagtt
    34741 gtacggcgca tccgacccgc gctcggtgga tgatttaacg gcggggtact aaggttagcg
    34801 gccctcttcg tgggaagagg gctattttgt cagggcaagc cgaaggtagc cttttttatt
    34861 tcgtaatcct gtagatattc ttccagcggc tttgccgttc ccagagaatg aatttcacca
    34921 ctgtctttat caataataaa aggtgcgtta ccagctaagc gcgcggcctc atctccagtt
    34981 tcgagaaatt ctcgtgcttc gaaacagaaa taccagccct ggctaaagcg tccatgtaga
    35041 gtaatgacga ccgggagatc tgcatcatca aggtaatggt tcgctttcgc gaatgcgtcg
    35101 tgataagtaa tcataattat aataaatatt attgttgagt gttatattat tattcctgtg
    35161 tgatacattg agcaaagacg cgttcatctt cgtcaaagat attattaaat gctggttttg
    35221 aaaaaagttc atcggataag atatagtcac gaaataagta tctgctaata ttttatgatt
    35281 tctttttcag aacgtcgaac gggattttct tatttaagat atcagaatct tatgattcgg
    35341 gtaaaattct aaat
//
LOCUS       DAZGJW010000036        35052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZGJW010000036 DAZGJW010000000
VERSION     DAZGJW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.036"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            198..1292
                     /gene="gcvT"
                     /locus_tag="ACHKIK_001792"
     CDS             198..1292
                     /gene="gcvT"
                     /locus_tag="ACHKIK_001792"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO9082979.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1316..1705
                     /gene="gcvH"
                     /locus_tag="ACHKIK_001793"
     CDS             1316..1705
                     /gene="gcvH"
                     /locus_tag="ACHKIK_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO9082980.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1824..4697
                     /gene="gcvP"
                     /locus_tag="ACHKIK_001794"
     CDS             1824..4697
                     /gene="gcvP"
                     /locus_tag="ACHKIK_001794"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO9082981.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            4958..5701
                     /gene="ygfF"
                     /locus_tag="ACHKIK_001795"
     CDS             4958..5701
                     /gene="ygfF"
                     /locus_tag="ACHKIK_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9082982.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYTASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5758..7191)
                     /gene="bglA"
                     /locus_tag="ACHKIK_001796"
     CDS             complement(5758..7191)
                     /gene="bglA"
                     /locus_tag="ACHKIK_001796"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO9082983.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRIHPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            7236..7547
                     /gene="yqfB"
                     /locus_tag="ACHKIK_001797"
     CDS             7236..7547
                     /gene="yqfB"
                     /locus_tag="ACHKIK_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO9082984.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            7711..8370
                     /gene="yqfA"
                     /locus_tag="ACHKIK_001798"
     CDS             7711..8370
                     /gene="yqfA"
                     /locus_tag="ACHKIK_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO9082985.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8566..9546)
                     /gene="ygfZ"
                     /locus_tag="ACHKIK_001799"
     CDS             complement(8566..9546)
                     /gene="ygfZ"
                     /locus_tag="ACHKIK_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO9082986.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9789..10055
                     /gene="sdhE"
                     /locus_tag="ACHKIK_001800"
     CDS             9789..10055
                     /gene="sdhE"
                     /locus_tag="ACHKIK_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO9082987.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10036..10443
                     /gene="ygfX"
                     /locus_tag="ACHKIK_001801"
     CDS             10036..10443
                     /gene="ygfX"
                     /locus_tag="ACHKIK_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO9082988.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10483..11004)
                     /gene="fldB"
                     /locus_tag="ACHKIK_001802"
     CDS             complement(10483..11004)
                     /gene="fldB"
                     /locus_tag="ACHKIK_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO9082989.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11116..12012
                     /gene="xerD"
                     /locus_tag="ACHKIK_001803"
     CDS             11116..12012
                     /gene="xerD"
                     /locus_tag="ACHKIK_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO9082990.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLRAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            12037..12747
                     /gene="dsbC"
                     /locus_tag="ACHKIK_001804"
     CDS             12037..12747
                     /gene="dsbC"
                     /locus_tag="ACHKIK_001804"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO9082991.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            12753..14486
                     /gene="recJ"
                     /locus_tag="ACHKIK_001805"
     CDS             12753..14486
                     /gene="recJ"
                     /locus_tag="ACHKIK_001805"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO9082992.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVVHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14577..15675
                     /gene="prfB"
                     /locus_tag="ACHKIK_001806"
     CDS             join(14577..14651,14653..15675)
                     /gene="prfB"
                     /locus_tag="ACHKIK_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO9082993.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15685..17202
                     /gene="lysS"
                     /locus_tag="ACHKIK_001807"
     CDS             15685..17202
                     /gene="lysS"
                     /locus_tag="ACHKIK_001807"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO9082994.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGVAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17245..17793)
                     /gene="idi"
                     /locus_tag="ACHKIK_001808"
     CDS             complement(17245..17793)
                     /gene="idi"
                     /locus_tag="ACHKIK_001808"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO9082995.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHRAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYGLGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            17848..17919
                     /locus_tag="ACHKIK_001809"
     CDS             17848..17919
                     /locus_tag="ACHKIK_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9082996.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(17916..18041)
                     /gene="yqfG"
                     /locus_tag="ACHKIK_001810"
     CDS             complement(17916..18041)
                     /gene="yqfG"
                     /locus_tag="ACHKIK_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO9082997.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(18043..19491)
                     /gene="uacT"
                     /locus_tag="ACHKIK_001811"
     CDS             complement(18043..19491)
                     /gene="uacT"
                     /locus_tag="ACHKIK_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO9082998.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            19927..21846
                     /gene="ygfT"
                     /locus_tag="ACHKIK_001812"
     CDS             19927..21846
                     /gene="ygfT"
                     /locus_tag="ACHKIK_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO9082999.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            21846..22334
                     /gene="ygfS"
                     /locus_tag="ACHKIK_001813"
     CDS             21846..22334
                     /gene="ygfS"
                     /locus_tag="ACHKIK_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO9083000.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(22370..23737)
                     /gene="ghxQ"
                     /locus_tag="ACHKIK_001814"
                     /gene_synonym="ygfQ"
     CDS             complement(22370..23737)
                     /gene="ghxQ"
                     /locus_tag="ACHKIK_001814"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO9083001.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(23773..25089)
                     /gene="guaD"
                     /locus_tag="ACHKIK_001815"
     CDS             complement(23773..25089)
                     /gene="guaD"
                     /locus_tag="ACHKIK_001815"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO9083002.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWARLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(25107..26507)
                     /gene="xanQ"
                     /locus_tag="ACHKIK_001816"
     CDS             complement(25107..26507)
                     /gene="xanQ"
                     /locus_tag="ACHKIK_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO9083003.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(26672..29542)
                     /gene="xdhD"
                     /locus_tag="ACHKIK_001817"
     CDS             complement(26672..29542)
                     /gene="xdhD"
                     /locus_tag="ACHKIK_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO9083004.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNSDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYNAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(29539..30318)
                     /gene="ygfM"
                     /locus_tag="ACHKIK_001818"
     CDS             complement(29539..30318)
                     /gene="ygfM"
                     /locus_tag="ACHKIK_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO9083005.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(30369..31697)
                     /gene="ssnA"
                     /locus_tag="ACHKIK_001819"
     CDS             complement(30369..31697)
                     /gene="ssnA"
                     /locus_tag="ACHKIK_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO9083006.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(31700..34798)
                     /gene="ygfK"
                     /locus_tag="ACHKIK_001820"
     CDS             complement(31700..34798)
                     /gene="ygfK"
                     /locus_tag="ACHKIK_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO9083007.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
BASE COUNT         8595 a         8900 c         9286 g         8271 t
ORIGIN      
        1 ttgcgatttt ggcgtttttt aaccataagc taatgtgatg atcaatttta ccttatggtt
       61 aacagtctgt ttcggtggta agttcaggca aaagagaacg attgcgttgg ggaccgggag
      121 tggctccgat gctgggtttc gtggtgataa tttcaccatg aaaaagttgt cagccccgct
      181 tattcaatga ggacaagatg gcacaacaga ctcctttgta cgaacaacac acgctttgcg
      241 gcgctcgcat ggtggatttc cacggctgga tgatgccgct gcattacggt tcgcaaatcg
      301 acgaacatca tgcggtacgt accgatgccg gaatgtttga tgtgtcacat atgaccatcg
      361 tcgatcttcg cggcagccgc acccgggagt ttctgcgtta tctgctggcg aacgatgtgg
      421 cgaagctcac caaaagcggc aaagcccttt actcggggat gttgaatgcc tctggcggtg
      481 tgatagatga cctcatcgtc tactacttta ctgaagattt cttccgcctc gttgttaact
      541 ccgccacccg cgaaaaagac ctctcctgga ttacccaaca cgctgaacct ttcggcatcg
      601 aaattaccgt tcgtgatgac ctttccatga tcgccgtaca agggccgaat gcgcaggcaa
      661 aagctgccac actgtttaat gatgctcagc gtcaggcggt ggaagggatg aaaccgttct
      721 ttggcgtgca ggcgggcgat ctgtttattg ccaccactgg ttataccggt gaagcgggct
      781 atgaaattgc gctgcccaat gaaaaagcgg ccgatttctg gcgtgcgctg gtggaagcgg
      841 gcgttaagcc atgcggtctg ggcgcgcgtg acacgctgcg tctggaagcg ggcatgaatc
      901 tttatggtca ggagatggac gaaactattt ctcctttagc cgccaacatg ggctggacta
      961 tcgcctggga accggcagat cgtgacttca tcggtcgtga agccctggaa gtgcagcgtg
     1021 agcatggcac agaaaaactg gttggtctgg tgatgaccga aaaaggcgtg ctgcgtaatg
     1081 aactgccggt acgttttacc gatgcgcagg gcaaccagca tgaaggcatt atcaccagcg
     1141 ggactttctc cccgacgctg ggttacagca ttgcgctggc acgcgtgccg gaaggtattg
     1201 gcgaaacggc gattgtgcaa attcgcaacc gtgaaatgcc ggttaaagtg acgaaacctg
     1261 tttttgtgcg taacggtaaa gccgtcgcgt gatttacttt tttggagatt gattgatgag
     1321 caacgtacca gcagaactga aatacagcaa agaacacgaa tggctgcgta aagaagccga
     1381 cggcacttac accgttggta taaccgaaca tgcccaggag ctgttaggcg atatggtgtt
     1441 tgttgacctg ccggaagtgg gcgcaacggt tagcgcgggc gatgactgcg cggttgccga
     1501 atcagtaaaa gcggcgtcag acatttatgc gccagtaagc ggtgaaatcg tggcggtaaa
     1561 cgacgcactg agcgattccc cggaactggt gaacagcgaa ccgtatgcag gtggctggat
     1621 ctttaaaatc aaagccagcg atgaaagcga actggaatca ctgctggatg cgaccgcata
     1681 cgaagcattg ttagaagacg agtaacggct ttattcctct tctgcgggag aggatcaggg
     1741 tgaggaaaat ttatgcctca ccctcactct cttcgtaagg agagaggttc acaattcact
     1801 gcacgtttca ggaaccatcg ctcatgacac agacgttaag ccagcttgaa aacagcggcg
     1861 cttttattga acgccatatc ggaccggacg ccgcgcaaca gcaagaaatg ctgaatgccg
     1921 ttggtgcaca atcgttaaac gcgctgaccg gccagattgt gccgaaagat attcagcttg
     1981 cgaccccgcc gcaggttggc gcaccggcga ccgaatacgc cgcactggca gaactcaagg
     2041 ctattgccag tcgcaataaa cgcttcacgt cttacatcgg catgggttac accgccgtgc
     2101 agctaccgcc ggttatcctg cgtaacatgc tggaaaatcc gggctggtat accgcgtaca
     2161 ctccgtatca acctgaagtc tcccagggcc gccttgaagc actgctcaac ttccagcagg
     2221 taacgctgga tttgactgga ctggatatgg cctctgcttc tcttctggac gaggccaccg
     2281 ctgccgccga agcaatggcg atggcgaaac gcgtcagcaa actgaaaaat gccaaccgct
     2341 tcttcgtggc ttccgatgtg catccgcaaa cgctggatgt ggtccgtact cgtgccgaaa
     2401 cctttggttt tgaagtgatt gtcgatgacg cgcaaaaagt gctcgaccat caggacgtct
     2461 tcggcgtgct gttacagcag gttggcacaa ccggtgaaat ccacgactac accgcgctta
     2521 ttagcgaact gaaatcacgc aaaattgtgg tcagtgtcgc cgccgatatt atggcgctgg
     2581 tgctgttaac tgcgccgggt aaacagggcg cggatattgt ttttggttcg gcgcaacgct
     2641 tcggcgtgcc gatgggctac ggtggcccac acgcggcctt ctttgcggcg aaagatgaat
     2701 acaaacgctc aatgccgggc cgtattatcg gtgtatcgaa agatgcagct ggcaataccg
     2761 cgctgcgcat ggcgatgcag actcgcgagc aacatattcg ccgtgagaaa gcgaactcca
     2821 acatttgtac ttcccaggtg ctgctggcaa acatcgccag cctgtatgcc gtttatcacg
     2881 gcccgattgg cctgaaacgt atcgctaacc gcattcaccg tctgaccgat atcctggcgg
     2941 cgggcctgca acaaaaaggt ctgaaactgc gccatgcgca ctatttcgac accttgtgtg
     3001 tggaagtggc cgacaaagcg ggcgtactga cgcgtgccga agcggctgaa atcaacctgc
     3061 gtagcgatat tctgaacgcg gttgggatca cccttgatga aacaaccacg cgtgaaaacg
     3121 taatgcagct tttcagcgta ctactgggcg ataaccacgg cctggacatc gacacgctgg
     3181 acaaagacgt ggctcacgac agccgctcta tccagcctgc gatgctgcgc gacgacgaaa
     3241 tcctcaccca tccggtgttt aatcgctacc acagcgaaac cgaaatgatg cgctatatgc
     3301 actcgctgga gcgtaaagat ctggcgctga atcaggcgat gatcccgctg ggttcctgca
     3361 ccatgaaact gaacgccgcc gccgagatga tcccaatcac ctggccggaa tttgccgaac
     3421 tgcacccgtt ttgtccgccg gaacaggctg aaggttatca gcagatgatt gcgcagctgg
     3481 ctgactggct ggtgaaactg accggttacg acgccgtttg tatgcagccg aactccggcg
     3541 cacagggcga atacgcgggc ctgctggcga ttcgtcatta tcatgaaagc cgcaacgaag
     3601 ggcatcgcga tatctgcctg atcccggctt ctgcgcacgg aactaacccc gcttctgcac
     3661 atatggcagg aatgcaggtg gtggttgtgg cgtgtgataa aaacggcaac atcgatctga
     3721 ctgatctgcg cgcgaaagcg gaacaggcgg gcgataacct ctcctgtatc atggtgactt
     3781 atccttctac ccacggcgtg tatgaagaaa cgatccgtga agtgtgtgaa gtcgtgcatc
     3841 agttcggcgg tcaggtttac cttgatggcg cgaacatgaa cgcccaggtt ggcatcacct
     3901 cgccgggctt tattggcgcg gacgtttcgc acctcaacct gcataaaact ttctgcattc
     3961 cgcacggcgg tggtggtccg ggtatgggac cgatcggtgt gaaagcgcat ctggcaccgt
     4021 ttgtaccggg tcatagcgtg gtgcaaatcg aaggcatgtt aacccgtcag ggcgcagttt
     4081 ctgcggcacc gttcggtagc gcctctatcc tgccaatcag ctggatgtac atccgcatga
     4141 tgggcgcaga agggctgaaa aaagcaagcc aggtggcaat cctcaacgcc aactatattg
     4201 ccagccgcct gcaggatgcc ttcccggtgc tgtataccgg tcgcgacggt cgcgtggcgc
     4261 acgaatgtat tctcgatatt cgcccgctga aagaagaaac cggcatcagc gagctggata
     4321 ttgccaagcg cctgatcgac tacggtttcc acgcgccgac catgtcgttc ccggtggcgg
     4381 gtacgctgat ggttgaaccg actgaatctg aaagcaaagt ggaactggat cgctttatcg
     4441 acgcgatgct ggctatccgc gcagaaatcg accaggttaa agccggtgtc tggccgctgg
     4501 aagataaccc gctggtgaac gcgccgcaca ttcagaacga actggtcgcc gagtgggcgc
     4561 atccgtacag ccgtgaggtt gcagtattcc cggcaggtgt ggcagacaaa tactggccga
     4621 cggtgaaacg tctggatgat gtttacggcg accgtaacct gttctgctcc tgcgtaccga
     4681 ttagcgaata ccagtaattc actgattcga ctattttcta aaggcgcttc ggcgcctttt
     4741 tagtcagatg acaaagtaca aaagtgctca gacagtcccc tcgccccttt ggggagaggg
     4801 tgaggggaac aggccggcac tggcgcgaat atttaccctc accccggccc tctccctgaa
     4861 agggcgaggg ggaaaagcgt gccaacattg aagattgagc cagtttgtta gcaatctcaa
     4921 agatacgtca acgaatgaat ttttctcgga aaaacaaatg gctatagcac ttgtgaccgg
     4981 tggcagtcgc ggcatcgggc gggcaactgc attactgttg gcgcaagaag ggtatacggt
     5041 ggcggttaat tatcagcaaa acctccacgc ggcgcaggaa gtgatgaact taataacgca
     5101 agccggtggc aaagcattcg tgctccaggc ggatatcagc gacgaaaacc aggtcgttgc
     5161 gatgtttaca gcaatcgatc agcacgatga accgctagca gcgctggtca ataacgccgg
     5221 gatcttgttt acccagtgca ccgttgaaaa ccttaccgca gagcgaatca accgagtact
     5281 ttccaccaac gtgacgggat attttctctg ctgccgcgag gcggtaaaac gcatggcgct
     5341 taaaaatggt ggcagtggcg gcgctatcgt caatgtctct tcggtggcct cacggttggg
     5401 ttcgccaggg gaatatgttg attacacggc atcgaaaggg gcgattgata cgttaaccac
     5461 cggactatcg ctggaagtcg ccgcgcaggg gatccgcgtt aactgcgtgc ggccagggtt
     5521 tatttatacc gaaatgcacg ccagcggcgg cgagcctgga cgcgtcgatc gcgttaagtc
     5581 gaacatcccc atgcagcgtg gtggacaggc agaagaggtc gcgcaggcca ttgtctggct
     5641 actaagtgat aaagcctctt acgtcacggg aagttttatc gatttggcgg gcgggaaata
     5701 aaacagggaa gttgtctgac cggatgcaac aagtattgca tccggtactt catcgactta
     5761 aagcttctcg ccgttgctgg caatcacctc ttggtaccag ttaaagctct tcttacgtga
     5821 acgcgacata tcgccagcac cgtcgtcatg tttattcaca tagataaagc cgtagcgttt
     5881 gctgtactgc ccggtggtga acgatacgca gtcgatgcag ccccacggtg tgtagcccat
     5941 cagatccacg ccatcgtaag tcaccgcttt tttcatctct tcgatatggg cgcgcaggta
     6001 gtcaatgcgg tagtcgtcgt tgatgctgcc atcttcttcc actttgtcgt aagcgccaaa
     6061 accgttttcg acaataaaca gcggcctctg ataacgctca tacagttcgc aaagggcata
     6121 gcgcagacct actggatcaa tctgccagcc ccagtcagac gctttaacat acgggttcgg
     6181 tacgctgcct tcgaaaccag agatcgcatc gccggtgccg ccttcggcct tcactgcgtt
     6241 ggtcatgtaa tagctgaaac caagataatc gcaggtgcct tcacgcagta catcgagatc
     6301 gccgtcttcc attttgatgt taaatccgcg acgctcccac tcgttcaaca cataggacgg
     6361 gtaatagccg cgtagctgca catcggtaaa gacgtagcgt tcgcgcatcg actcctgagc
     6421 gaacatcaca tcgtccgggt tacagcagta gggatagagc ggcaccatcg ccagcataca
     6481 gccgactttc atctccgggt gaatgcgacg cgcagctttc accgccaggg cgctggcgac
     6541 aaactggtga tgcagcacct gatacatcgt ctcttccggg ttctcatgct cggtataaac
     6601 cacgccggag cagcagtaac cgaacagcgg tgcacgccag ttacgctggt tgttaatttc
     6661 gttgaaggtc atccagtatt tgactttatg cttatagcgt tcaaacacca cttccgcgaa
     6721 acggacaaag aaatcaacca ctttacggtt ggtccagcta ccgtattgct gcaccagatg
     6781 cagcggcatt tcaaagtggg agagggtgat caccggttcg atgttgtatt tcagcagttc
     6841 atcgaacata tcatcgtaga acttcagccc ttcttcgttt ggctgagctt catcaccttt
     6901 tggaaaaata cgcgtccagg cgatggaagt ccgaaaacat ttgaagccca tttcggcaaa
     6961 tagcttgatg tcttccttgt agtgaccata aaaatcaacg gcttcatggt ttggatagta
     7021 tttgcctggc aagacttctt tggtgatttc gcgcggcacg ccgtgtgcgc caccggtcag
     7081 aacgtcacaa atgctcggcc cttttccgcc tttgttccag ccgccttcga cctgatgagc
     7141 ggcaactgcg ccgccccata agaaatcttt cggtaaggtg agttttttca ctatcatagg
     7201 ctccagatga gtcaattctt acaggaaata gtaccatgca gccaaacgac atcacttttt
     7261 ttcaacgttt ccaggatgac attctggctg ggcgtaaaac catcaccatc cgcgacgagt
     7321 ctgaatcgca cttcaaaacg ggtgatgtgc ttcgtgtcgg acgttttgaa gatgacggtt
     7381 atttttgcac gattgaagtc accgcaacct caaccgtaac gctggatacg ctgactgaaa
     7441 aacatgcaga gcaggaaaat atgaccctga ctgaactgat aaaggtcatt gccgacatct
     7501 atcccggtca gacacagttt tatgtgattg aatttaaatg tctttaaatt gaactcgcgc
     7561 ctgttagttg aaatttcgca tttaatttca tgatttttag gagattagtc tcaaatcaga
     7621 atttatttta gctaacaggt gttcactgga actattctca gttacgctgg agaggtatac
     7681 atgtgcgtgt acgtttctcc ggagtaagtt atggttcaga agcccctcat taagcaggga
     7741 tattcgctgg cagaggaaat tgccaacagc gtcagtcacg gcattgggtt ggtgtttggt
     7801 atcgttgggc tggtgttgct actggttcag gcggtggatc tcaatgccag cgccactgcg
     7861 ataaccagtt acagcctcta tggcggcagt atgatcctgc tgttcctcgc ttcgacgctc
     7921 tatcacgcca ttcctcatca acgggcaaaa atgtggctga agaaatttga ccattgcgct
     7981 atttacctgt tgattgccgg aacctacacg ccgtttttgc tggtggggct ggattctccg
     8041 ttagcgcgcg ggttgatgat tgttatctgg agcctggcat tgctgggtat tctgtttaaa
     8101 ctgaccatcg cgcaccgatt caaaatttta tctctggtga cctatctggc gatgggctgg
     8161 ctgtcgctgg tggtaattta tgaaatggca gttaagctcg cggcgggcag cgttacctta
     8221 ctggcggtag gcggcgtggt ttattcgctc ggggtgattt tctacgtctg caaacgcatt
     8281 ccatacaacc atgccatctg gcacggcttc gtgctcggcg gtagtgtgtg ccactttctg
     8341 gcgatctatt tgtatattgg gcaggcgtaa tgggtatgcc tttgtaggcc tgataagacg
     8401 cgccagcgtc gcatcaggca tcggagcaca actgccggat tcggcgtgaa cgccttatcc
     8461 gatctacggg cctcgtccct gttgtgataa aggcatcaaa tcccagacat gataagacgc
     8521 gtcagcgtcg catcaggcga taaatgcctg atgcgataaa gagatttact cttcgagcga
     8581 atacggcagc ggctcgatat gcaatgtatt cgcatcgtcg cgtacgcgga agatgctatc
     8641 cggttccata tcgttattca tgacgacctg taccacgacc tgaccatctt ccagttttac
     8701 cgcagccagc accgtaccgg tacgacgcca gttctcgccc attttcagct ctaagtcttc
     8761 accagcttcc ggcagtcggc tggcgctacc tgccagcaac cagagcgcac gtttattggc
     8821 accacggaat tttgctcgcg ccaccatctc ttgtccggta taacagcctt tcttaaagct
     8881 gataccgccc agcgcctgga gattggtcgc ctgtgggata aactgcccgc tgttggcggc
     8941 atcaatcacc gggaaacccg cttcaatgtt taatgccagc cactgttggc tattgttcag
     9001 ttccgcttca ccgcgcagtt tatcggtcag catattagca gtagcttcat cggttacgat
     9061 caggaaacgt tctgccgggt gttcaaacca tagcaaagtg gtcgcgcctt ctttgactac
     9121 ctgtttttct ttcgaaggca gttcgctaaa gagatttgcc agcgcggcgc gcgcctgaaa
     9181 accggcaaca ccaagcagca cacgctcgtc gtctggcgcg atggtcactt tagagaatac
     9241 cgcatatttt ttcagttcag tcagctgcgg ttcacgcacg ctgcgccgtt caatccatgc
     9301 aaagccatcg ccgtcgcgga acagacgtaa attgctccac attttacctt tggcgtcgca
     9361 atgggcggcg agcaggtgct gattttctgt catctggctg acatctgctg tcacctgacc
     9421 ctgcatatat ttttcgctgt ccgcgccagt aatggtggca agcgcccagt catcaagcgt
     9481 catcagcgtc agcggtaaac gggcagaagc cgtaggctga cggggaggaa aaggtgtaaa
     9541 agccataata gagtcctgag ttgcttaacg gcgtattaat gactaatggt aaaagagcta
     9601 tgaggcaatg caagtgcttt cactacgcga tctgtgcctt ctgcgaggcg acttccaaaa
     9661 aaatgaagcc attgaattgt tgtgaattaa tttcgcgatc gcgatggtgt ttactgatat
     9721 tcccgtaaaa gaaggcgaaa aacacgttac aatagcgggg tactggattt tcgtgagaca
     9781 ggaagaagat ggacattaac aacaaagccc gcattcattg ggcatgccgc cgtggtatgc
     9841 gcgaactcga tatttcaatc atgccgtttt tcgaacatga gtacgacagc ttaagcgatg
     9901 acgaaaaacg catctttatt cgtctgctgg aatgtgacga tccggacctg tttaactggc
     9961 tgatgaatca cggtaaaccg gccgatgcag aactggaaat gatggtccga ctcatccaga
    10021 cacggaaccg ggaacgtggt cctgtggcaa tctgatttgc gcgtctcctg gcgcgcacag
    10081 tggctttcct tgctgattca tgggctggtt gccgctgtta ttttactcat gccctggcca
    10141 ctcagttaca ccccgttatg gatggtgtta ctttcgctgg tggtgtttga ttgcgttcgc
    10201 agccagcggc gtattaatgc tcgccagggg gaaattcgct tgttgatgga cgggcgtttg
    10261 cgttggcaag ggcaggagtg gagcatcgtc aaagcaccgt ggatgattaa gagcggcatg
    10321 atgctgcgtt tacgttctga tggcggtaaa cggcaacatt tatggctggc agccgacagc
    10381 atggacgaag ctgaatggcg ggatttacgg cggattttgt tgcaacaaga gacgcaaaga
    10441 taaagatgac ggcaaagcat atgcgctaag ccgtcagtga gatcaggcgt aatgctctgc
    10501 catttcgttg aggatttgct cgcaccagct ctgaatacgc tcgtcgctaa ggtcatactg
    10561 gttagtttca tccagcgcca gacccacgaa cagttgcccg tcagcaatca ccggtttcgg
    10621 gctggtaaat tcatatcctt ccgttggcca gtagccgacg aacttcacgc ctttggtcga
    10681 gagtttgtca tgcagcatac cgagcgcatc gaggaaccac tcgccgtatc ccagttgatc
    10741 gccaagccca tacagcgcaa caattttacc ttcaaggttc aggtcgtcga gctgatccca
    10801 gacggcttcc cagtcttcct ggatttcacc aaaatcccag gtcgggatac ccagaatgag
    10861 cacatcgtac tgctccatta atttcgggga gtcgtccttg aggttatgta aggtcaccag
    10921 ttctgggccg ataatatcgc ggattttttc tgccgccatt tcggtgtaac aggtgctgga
    10981 accgtaaaaa agacccatat tcataacgta agcttctcga ttctgctgtg gcttttgcag
    11041 ctagtgtatc agaatcgctt tagtcacggc acaataaggc ataatggatc aaatgagtga
    11101 ccataagggg cgcaagtgaa acaggatctg gcacgcatcg agcagtttct tgatgctctg
    11161 tggctggaaa aaaatctggc tgaaaatacg ttgaacgctt accgtcgcga tctgtcaatg
    11221 atggtggagt ggttgcatca ccgcgggttg acgctggcga cggcgcaaag tgacgatttg
    11281 caggcattac tggcagaacg gctggagggc gggtataaag ccaccagctc agcgcgtttg
    11341 ctgagtgcag tgcgccgatt gttccagtat ctttatcgcg aaaagtttcg tgaagacgat
    11401 cccagtgcgc atctcgcttc accgaaattg ccccagcgtt tgccaaaaga tttaagcgaa
    11461 gcgcaggtcg aacgtttatt acgggcacca ttaattgatc agccactgga gctacgcgat
    11521 aaagccatgc ttgaagtgtt gtatgctacc gggctgcgtg tctctgaact ggtcggactg
    11581 acaatgagtg atatcagcct gcgtcagggc gtagtgcggg tcattggtaa aggcaacaaa
    11641 gagcgcctgg taccgttagg tgaagaggcg gtttactggc tggaaaccta tctggaacat
    11701 gggcgtccgt ggctgctgaa tggtgtgtca attgacgtgt tgtttcccag ccagcgtgcg
    11761 cagcagatga cgcgacagac cttctggcac cgcattaaac attatgctgt gctggcgggt
    11821 atcgacagcg aaaagctgtc accgcatgtg ttgcgtcacg cttttgccac tcatttatta
    11881 aatcatggtg cggatttacg cgtggtgcag atgctactgg gccacagcga tctttccacc
    11941 acgcaaattt atacgcatgt cgctaccgag cgtctgcggc aacttcatca acagcatcac
    12001 ccgcgggcgt gatgtctgaa aagaacggga agatttatga agaaaggttt tatgttgttt
    12061 actttgttag cggcgttttc aggttttgct caggctgatg acgcggcaat tcaacaaacg
    12121 ttagccaaaa tgggcatcaa aagcagcgat attcagcccg cgcccgtagc tggcatgaag
    12181 acagttctga ctaacagcgg cgtgttgtac atcaccgatg atggtaaaca tatcattcag
    12241 gggccaatgt atgacgttag cggcacggct ccggtcaatg tcaccaataa gatgctgtta
    12301 aagcagttga atgcgcttga aaaagagatg atcgtttata aagcgccgca ggaaaaacac
    12361 gtcatcaccg tgtttactga tattacctgt ggttactgcc acaaactgca tgagcaaatg
    12421 gcagactata acgcgctggg gatcaccgtg cgttatcttg ctttcccgcg ccaggggctg
    12481 gacagcgatg cagagaaaga aatgaaagct atctggtgtg cgaaagataa aaacaaagcg
    12541 tttgatgatg tgatggcagg taaaagcgtc gcaccagcca gctgcgacgt ggatattgcc
    12601 gaccattacg cacttggcgt ccagcttggc gttagcggta ctccggcagt tgtgctgagc
    12661 aatggcacac ttgttccggg ttaccagccg ccgaaagaga tgaaagaatt cctcgacgaa
    12721 caccaaaaaa tgaccagcgg taaataattc gcgtgaaaca acagatacaa cttcgtcgcc
    12781 gtgaagtcga tgaaacggca gacttgcccg ctgaattgcc tcccttgctg cgccgtttat
    12841 acgccagccg gggcgtgcgc agtgcgcaag aactggaacg cagtgttaaa ggtatgttgc
    12901 cctggcagca actgagcggc gtcgaaaagg ccgttgagat cctttacaac gcttttcgcg
    12961 aaggaacgcg gattattgtg gtcggtgatt tcgacgccga cggcgcgacc agcacggctc
    13021 taagcgtgct ggcgatgcgc tcgcttggtt gcagcaatat cgactacctg gtaccaaacc
    13081 gtttcgaaga cggttacggc ttaagcccgg aagtggtcga tcaggcccat gcccgtggcg
    13141 cgcagttaat tgtcacggtg gataacggta tttcctccca tgcgggggtt gtacacgctc
    13201 gctcgttggg cattccggtt attgttaccg atcaccattt gccaggcgaa acattacccg
    13261 cggcggaagc gatcattaac cctaacttgc gcgactgtaa tttcccgtcg aaatcactgg
    13321 caggcgtggg tgtggcgttt tatctgatgc tggcgctgcg cacctttttg cgcgatcagg
    13381 gctggtttga tgagcgtggc atcgcaattc ctaacctggc agaactgctg gatctggtcg
    13441 cgctgggaac agtggcggac gtcgtgccgc tggacgctaa taatcgcatt ctgacctggc
    13501 aggggatgag tcgcatccgt gccggaaagt gccgtccggg gattaaagcg ctgctggaag
    13561 tggcaaatcg cgatgcgcaa aaactcgccg ccagcgattt aggttttgcg ctggggccac
    13621 gtctcaatgc tgccgggcga ctggacgata tgtccgtcgg tgtggcgctg ttgctgtgcg
    13681 acaacatcgg cgaagcgcgc gtgctggcaa atgaactcga tgcgctaaac cagacgcgaa
    13741 aagagatcga acaaggaatg caagttgaag ccctgaccct gtgcgagaaa ctggagcgaa
    13801 gtcgcgacac gctacccggc gggctggcaa tgtatcaccc cgaatggcat cagggcgttg
    13861 tcggtattct ggcttcgcgc atcaaagagc gttttcaccg tccggttatc gcctttgcgc
    13921 cagcaggtga tggtacgctg aaaggttcag gtcgctccat tcaggggctg catatgcgtg
    13981 atgcactgga gcgattagac acactctacc ctggcatgat actgaagttt ggcggtcatg
    14041 cgatggcggc gggtttgtcg ctggaagagg ataaattcga actctttcaa caacggtttg
    14101 gcgaactggt tacagagtgg ctggaccctt cgctattgca aggcgaagtg gtgtcagacg
    14161 gtccgttaag cccggccgaa atgaccatgg aagtggcgca gttgctgcgc gatgctggcc
    14221 cgtgggggca gatgttcccg gagccgctgt ttgacggtca tttccgtctg ctgcaacagc
    14281 ggctggtggg cgaacgtcat ttgaaggtga tggtcgaacc ggtcggcggc ggtccactgc
    14341 tggatggtat tgcttttaat gtcgataccg ccctctggcc ggataacggc gtgcgtgaag
    14401 tgcaactggc ttataagctc gatatcaacg agtttcgcgg caaccgcagc ctgcaaatta
    14461 tcatcgacaa tatctggcca atttagcgtc atcttctcta taaaaaagag cgtggattgg
    14521 gtacaatccc gctcttatca ccgcattttg actagctcaa taaaagaaat cagaccatgt
    14581 ttgaaattaa tccggtaaat aatcgcattc aggacctcac ggaacgctcc gacgttctta
    14641 gggggtatct ttgactacga cgccaagaaa gagcgtctgg aagaagtaaa cgccgagctg
    14701 gaacagccgg atgtctggaa cgaacccgaa cgcgcacagg cgctgggtaa agagcgttcc
    14761 tccctcgaag ccgttgtcga caccctcgac caaatgaaac aggggctgga agatgtttct
    14821 ggtctgctgg aactggctgt agaagctgac gacgaagaaa cctttaacga agccgttgct
    14881 gaactcgacg ccctggaaga aaaactggcg cagcttgagt tccgccgtat gttctctggc
    14941 gaatatgaca gcgccgactg ctacctcgat attcaggcgg ggtctggcgg tacggaagca
    15001 caggactggg cgagcatgct tgagcgtatg tatctgcgct gggctgaatc gcgtggtttc
    15061 aaaactgaaa tcatcgaaga gtcggaaggt gaagtggcgg gtattaaatc cgtgacgatc
    15121 aaaatctccg gcgattacgc ttacggctgg ctgcgtacag aaactggcgt tcaccgcctg
    15181 gtgcgtaaaa gcccgtttga ctccggcggt cgtcgccaca catcgttcag ctctgcgttt
    15241 gtttacccgg aagttgatga tgatattgat atcgaaatca acccggcgga tctgcgcatt
    15301 gacgtttatc gcgcgtccgg cgcgggcggt cagcacgtta accgtaccga atctgcggtg
    15361 cgtattaccc acatcccaac cgggatcgtg acccaatgcc agaacgaccg ttcccagcac
    15421 aagaacaaag accaggccat gaagcagatg aaagcgaagc tttatgaact ggagatgcag
    15481 aagaaaaatg ccgagaaaca ggcgatggaa gataacaaat ccgacatcgg ctggggcagc
    15541 cagattcgtt cttatgtcct tgatgactcc cgcattaaag atctgcgtac cggggtagaa
    15601 acccgcaaca cgcaggccgt gctggacggc agcctggatc aatttatcga agcaagtttg
    15661 aaagcagggt tatgaggaac caacatgtct gaacaacacg cacagggcgc tgacgcggta
    15721 gtcgatctta acaatgaact gaaaacgcgt cgtgagaagc tggcgaacct gcgcgagcag
    15781 ggggttgcct tcccgaacga tttccgtcgc gatcatacct ctgaccaatt gcacgcagaa
    15841 ttcgacggta aagagaacga agaactggaa gcgctgaaca tcgaagtcgc cgttgctggc
    15901 cgcatgatga cccgccgtat tatgggtaaa gcgtctttcg ttaccctgca ggatgtgggt
    15961 ggccgcattc agctgtacgt tgcccgtgac gatctgccgg aaggcgttta caacgagcag
    16021 ttcaaaaaat gggacctcgg cgacatcctc ggcgcgaaag gtaaactgtt caaaaccaaa
    16081 accggcgaac tgtctatcca ctgcaccgag ctgcgtctgc tgaccaaagc actgcgtccg
    16141 ctgccggata aattccacgg cttgcaggat caggaagcgc gctatcgtca gcgttatttg
    16201 gatctcatct ctaacgatga atcccgtaac acctttaaag tacgctcgca gatcctctct
    16261 ggtattcgcc agttcatggt gaaccgcggc tttatggaag ttgaaacgcc gatgatgcag
    16321 gtgatcccag gcggtgccgc tgcgcgtccg tttatcaccc accataacgc gctggatctc
    16381 gacatgtacc tgcgtatcgc gccggaactg tacctcaagc gtctggtggt cggtggcttc
    16441 gagcgtgtat tcgaaatcaa ccgtaacttc cgtaacgaag gtatttccgt acgtcataac
    16501 ccagagttca ccatgatgga actctacatg gcttacgcag attacaaaga tctgatcgag
    16561 ctgaccgaat cgctgttccg tactctggca caggatattc tcggtaagac ggaagtgacc
    16621 tacggcgacg tgacgctgga cttcggtaaa ccgttcgaaa aactgaccat gcgtgaagcg
    16681 atcaagaaat atcgcccgga aaccgacatg gcggatctgg ataacttcga ctctgcgaaa
    16741 gcgattgctg aatctatcgg tatccacgtt gagaagagct ggggtctggg ccgtatcgtt
    16801 accgagatct tcgaagaagt ggcagaagca catctgattc agccgacctt cattactgaa
    16861 tatccggcag aagtttctcc gctggcgcgt cgtaacgaca ttaacccgga aatcacagac
    16921 cgctttgagt tcttcatcgg tggtcgtgaa atcggtaacg gctttagcga gctgaatgac
    16981 gcggaagatc aggcgcaacg cttcctggat caggttgccg cgaaagacgc aggtgacgac
    17041 gaagcgatgt tctacgatga agattacgtt accgcactgg aacatggctt accgccgaca
    17101 gcaggtctgg ggattggcat cgaccgtatg gtaatgctgt tcaccaacag ccataccatc
    17161 cgcgacgtta ttctgttccc ggcgatgcgt ccggtaaaat aagcattacg ttatgctcac
    17221 aaccccggca aatgtcgggg ttttttaatt gtgctgcgcg aaagcagaca acaattttct
    17281 tgcttcacta ttggctgcct gcatcaccat ccacggactg aacgcccacg gtgtggcatc
    17341 aataccgtgt aatacatctg ctaaatcaca ccattgataa tccatcactt catcatcgtt
    17401 gatctgtaac gcactggtgg tgcgtgcggc aaataccgga cacacttcat tttccacaat
    17461 gccattcgga tcggtggcgc gatagcgaaa gtcaggatag atagattcag gaggcgtaat
    17521 ttccacgcca agcccataac ggcaacggcg gatcaccgcg tcttcgttgc tttctcccag
    17581 ttgtgggtgc ccacaaaccg agttagtcca cacgccaggc cacgcttttt tgctcagtgc
    17641 gcggcgggta actaataatt gtcctttggc attaaacagc caactggaga acgcgcgatg
    17701 taagcgggtg tctgccgtgt gtgcggcata cttttccagc gtacccgtgg gaactccctg
    17761 tgcattcaat aaaatgacgt gttccgtttg cataatttct cacatgtaat tctgatcact
    17821 catcattata gataattgaa gagtgaaatg attaaccaag tgagcgttta tcgacaaccg
    17881 cccgttttga gcggatgccg acaggtaaaa accatttaaa aaagtagcat catatatttg
    17941 aagcgacttt caatggccat tgcaacacag tcagaaatga caggaataga gagagtaaaa
    18001 agcaacaaca gactgaatat agcgcgcatt aaaaaattca tattattctc catgctcatt
    18061 tttcttcagc ttgcgcatca gaagccacat acgtactgtc ctgactttta aatctttatc
    18121 agagacggat tccttcacca ggtcagcatg atgctgatag ccattaaaga agacattcag
    18181 cacaacggcg ctaagggttg cgagcataat gccgctatgc agcaacggtt gcagtacggc
    18241 cggtaactta gaaaagaaat cgtgagagag cgtcggagtc atgccaacgc cgagactgat
    18301 cgccacaata tagaggttgt aacggttggt ggtgtagtta cagcgcgaca gaattcgaat
    18361 ccctgtcgcc agtaccatgc cgaacatcac cagcccagcg ccgcccagca caaattgcgg
    18421 aatggaggct accagcaccg ccatttttgg caccatgcca aacaggatta atataattcc
    18481 cgaagaaata cacacccagc ggctatgaac gcgcgtcacg ctaaccaggc caacgttttg
    18541 agaaaatgac gtgtggggga agctgttaaa cgtgccgcct atcattgtcc ctacgccatc
    18601 gacacgcagc ccgcgaataa tatcgtgtga agagagttta cgaccgacta tttcacccag
    18661 tgccaggaac atccccatcg actcgataaa cacgatgatt aacacggcag tcatggtcag
    18721 aatggaaacg ggatcgaaaa tcggcatacc aaacgacatc ggggtgacaa tcgcaaacca
    18781 cgaggcatca tgtagcccgg ataaattgac ttcattcatc atccacgaaa gtaaaaagcc
    18841 aaatacaatc cccagtaata cggcgacgtt ggacataaat cctttcgcat agcgagtaat
    18901 gagcaagata aaaattaaga cggcaaagga gatacctaaa taaacgggat taccatattg
    18961 cggattccct ttacctccgg cggcccagtc aatacccacc tgaatgatgc taagcccaat
    19021 agaagtaata accacaccgg taaccagtgg cgggaataaa ggcatcaagc gaccaataag
    19081 tggcgctaat aatgtggtga taaaacccgc ggctatagtg gcaccaaata tccccagcag
    19141 gccgatatcc gggttcatcc caatggctat cattggtgtt acagcagcaa aggtcaccga
    19201 cataatcacc ggcaggcgga tccccataaa gcggccgata ccgatacatt gcaataatgt
    19261 gacgatcccg cagcaaaaga gatccgagct aatgagcatc gcaatagctt cttttgagag
    19321 gcccagtcgg tcaccaatca taagaggaac agcgactgca cctgcgtaca tgacaaggac
    19381 gtgttgcaga ccgagtatga tcagctttcc tggtgataat atgcggtcaa cctcatcagt
    19441 tgggcggtct tgcccagaag atgagggaag ttgggaatct atggcgctca tcagtgtttc
    19501 tcctttcgcc cgccagagta aaaagcaacc gtttacgtta atgaaactgt ctaaacacat
    19561 gctcgtggag tgccagtttt tgcgaggaag gggagttaga gacaggaatt gcggaggcaa
    19621 tcacataatc gtggttaaac cacgaaaatg agaagagaac ctgacaatga aattatctgt
    19681 aattttctca tattgatatt tcattaatca ttctggcatg tcgcactctc gcatttaatc
    19741 gtttttatct ggatagcgct cttttggtcg gcaatgttga aataatgaaa gcggcataga
    19801 ttgaggggca ataatttgaa ctcagtcgca aaattataat taaaacggtc atgctaccta
    19861 atatatgccc gtttattgca tcgcgaaatt tgagaagaca taactcatta aatgaaaggg
    19921 atgcaaatga ataagtttat cgctgctgaa gctgcggaat gtataggctg tcatgcttgt
    19981 gaaattgcct gtgcggtggc acacaatcaa gaaaactggc cgctgagtca cagtgacttt
    20041 cgaccgcgta tccacgttgt agggaaaggc caggctgcga atccggtggc ctgccatcac
    20101 tgcaacaatg ccccttgcgt tacggcttgt ccggttaatg ctctgacttt ccagtccgat
    20161 agcgtacaac tggacgagca aaaatgtatt ggctgtaaaa gatgcgcaat cgcttgcccc
    20221 tttggcgtcg ttgagatggt cgatacgatt gcgcagaaat gcgacctttg taaccagcgc
    20281 agttccggca cgcaagcctg tattgaagtc tgcccaacac aggcgttacg actgatggac
    20341 gataaagggt tacagtggat aaaggtggct cgccagcgca aaacggcagc aggaaaagcg
    20401 tcatcagatg ctcagccatc tcgcagtgca gcgttgctcc ccgttaactc gcgtaaaggc
    20461 gcagataaaa tttcagcgag tgaacggaaa acccactttg gcgaaatata ttgcgggctg
    20521 gatccactgc aagcgactta tgagagtgac cgctgtgttt attgtgccga aaaagctaac
    20581 tgcaactggc attgcccgct gcataacgct attccggatt acatccgtct ggtacaggaa
    20641 ggaaagatta ttgaagcggc agaactttgc caccagacca gttccttacc agaaatttgc
    20701 ggcagggtat gtccacagga ccgtctttgt gaaggcgcat gtactttgaa agatcactcg
    20761 ggcgcagtct ctatcggtaa tctggaacgc tacatcaccg ataccgcgct ggcgatgggc
    20821 tggcgtcccg atgtcagcaa agttgttccc cgtagcgaaa aagtggcggt gattggcgct
    20881 gggccagcag ggttagggtg tgctgatatt ctggcgcgcg cgggagttca ggttgatgtc
    20941 tttgatcgcc atccagaaat tggcggtatg ctgacttttg gcattcctcc tttcaaactc
    21001 gataaaacgg tattaagcca gcggcgagag atattcaccg caatgggaat cgactttcat
    21061 cttaactgtg aaattggccg cgatattacc tttagcgatt taacttctga atatgatgca
    21121 gttttcatcg gcgtggggac ttacgggatg atgcgagcag atctgccgca tgaagatgcg
    21181 cccggtgtca ttcaggctct accgttcctg actgcccata cccgccagct catgggattg
    21241 ccggagtctg aagagtatcc gctgacggac gtggaaggta agcgagtcgt ggtattgggc
    21301 ggtggcgata cgacaatgga ttgtttgcgg acttccatcc gcctcaatgc cgccagcgtg
    21361 acctgcgcgt atcgtcgtga tgaagtcagt atgccgggct cgcgcaaaga ggtggttaat
    21421 gcgcgcgagg aaggtgtcga gtttcagttc aatgttcaac cgcaatatat cgcttgtgac
    21481 gaagatgggc gtttaactgc ggtgggcctg attcgtaccg ccatgggtga gccggggccg
    21541 gatggtcgcc gtcgtcctcg tccggtagcg ggttcagagt ttgaattgcc cgccgatgtt
    21601 ctgattatgg cctttggttt ccaggcgcat gccatgccgt ggttgcaggg cagcggaatt
    21661 aaactcgata aatggggcct gattcaaacc ggtgacgtcg ggtatttacc tacccagacg
    21721 catctgaaaa aagtctttgc tggtggtgat gcagttcatg gcgcggatct ggttgtcact
    21781 gcaatggccg caggaaggca ggcggcgcgc gatatgttaa ctctgtttga tacgaaggca
    21841 tcgtgatgaa atcgttaatt atcgttaatc cggctgactg tattggctgc cgtacttgtg
    21901 aagtagcctg tgtggtcgct cacccttcag aacaggagtt gaatgccgat gtctttttac
    21961 cccggctgaa ggtacagcga ctggacagca tcagtgcgcc ggtgatgtgc catcagtgtg
    22021 aaaacgcccc ttgtgttggc gcttgccccg tgggggcgct gacgatgggc gagcaggtgg
    22081 tgcagaccaa ttctgcccgt tgtattggct gtcagagttg cgtcagtgcg tgcccgtttg
    22141 gaatgatcac cattcagtcg ttgccggggg atacccgaca acaaattgtg aaatgcgatc
    22201 tctgtgaaca gcgggaagag gggccagcct gtgttgaatc ttgcccgaca caggcgctgc
    22261 agttgcttac tgaaagagaa ctcaggcgag tccgccagca gcgtattgtt gccagcggtg
    22321 agaatcctct gtgaaagaaa tctccgcgct gaaaggcgcg gagaatctat tagattgccc
    22381 aaccacccgc gtaaaatgcg accagtgcgg cagtaatgat caccgtacca atattcaact
    22441 tttgccattc gcgagcaaag acacggccta cgaccagtgt cacaaagccc agcataatac
    22501 cggtaacgat attacaagtc agaacgatga acacggcaca caccaggcca gccattgcgt
    22561 caataaaatc gttgaaatcc agcttcgaga cgttacttaa catcagcaaa cccacgtaca
    22621 tcagagcggg tgcagtggcg taaccaggga tcaaaaatga cagcggtgat aagaacagaa
    22681 tcagcaggaa taacgccccc actacggttg cggtcaaccc tgttttaccc ccggcggcgg
    22741 ttcctgccgc tgattcgata taaaccgctg cgggcgctgc gcccaccaga ccggagaata
    22801 ttgaacttac tgagtcactg gtcagggctt tgccgccatt gatgatctgg ttgtctttat
    22861 ccaacaaatt cgcctgaccg gcgacggcac ggatggtgcc agtggcgtcg aacactgcgg
    22921 tcatcaccaa tgccagtaca ctcggaagta cggttggctg gagtgcgccc ataatatcga
    22981 ggctgaaaat cagagactta ccatcttcgc cagtcagact tggcatcgcc accagaccgt
    23041 ggtatttcac cgctgggtca aagattaagc cgataatcga aattgcgata atcaccaaca
    23101 agatcccgcc gggtacgcgg cacttctcca gaccgaagat gaccgccagc cccagcaagc
    23161 tcatcatcac cgggaaggag gtaaacgcac cgagcgccac tggcaagcct tcaatcgggt
    23221 ttttgataac catacccaca ccgttagcag caatcagcag cagaaacagg ccgataccga
    23281 tacctgtacc gtgagcgata cccatcggta aattacgtaa gatccaggta cgcacaccgg
    23341 ttacggaaat agcggtgaag atgacgccca tcagaaagac cgcgcccagt gcgacaggaa
    23401 cgctaatttg ttgcccgagt accagactga atgcggtaaa tgccgtcaag gaaatcgcac
    23461 aaccaatcgc cattggcaaa ttggcccaca accccatcag caacgagccg aagcccgcga
    23521 ccagacaggt ggcaacaaac acagctgcgg gaggaaaacc tgctttgccc agcattcccg
    23581 gaacgacgat aacggaataa accatggcca gaaaggtcgt taagccagcc agtacttcct
    23641 gacgaacggt actgccacga gcggtaattt taaaataatt atccagcgcg ccctgtggct
    23701 ttggtgcgtc cggtgtttgt aggatgtctc cagacataat gatgtcctca cgaggatgtt
    23761 aaagttttat tattagttgc gttcgtacac cagacgacca tcaacgtagg tgcggtagat
    23821 cgaacggtca tcgcccaacg tcatcatcac gaacaatttg tcgactaaag aaacagagtt
    23881 gtcatagcgc agctgctgta gcggagtggc ggtgggttcc atcaccacga aatcagcctc
    23941 tttgccaggt aaaaagttgc caatcaaatc gtcaaggccc agagatttcg ctccgccgag
    24001 cgtggccagg taaaacgctt catatgccga gaggcgatag ccttgtaatt gcaatacttt
    24061 gtaggcttcg ttcagcgttt gcagcatgtt gaaagtggtt ccggcaccga tatccgttcc
    24121 catgcccact ttaactttct tctgccatgc ttttttcaag ttgaataagc cgctgccgag
    24181 gtaaaggttg gaggtcggac agaaagcaat gctggatttg gtttcgctga gacgagccca
    24241 ctctttttct tcgagatgga cgcagtgagc aaagacacag tttttaccgg tcaggccgta
    24301 ctgatggtaa acatccagat aaccatcatg gtcaggataa agcgatttca cccaggcaat
    24361 ttcatctttg ttttcacaga gatgggtatg tacccacgta tccggatatt cttctttcag
    24421 gcgttgcgcc atcgccatct gttcaggaga tgaggtcggg gcgaagcgtg gcgtaatcgc
    24481 atatagcaga cgaccatttt tgtgccagcg ttcaatcagt tctttgcttt ggtgatagct
    24541 gctttcggca gtgtcgagca gataatccgg tgcgttgcgg tccatcatca ccttaccggc
    24601 aatcatacgc atattgatat gactggcggc ttcaaacagc gcatcaacag attgcggatg
    24661 aacagtgcca aacaccagcg ccgtggtggt tccgttacgt aaaagctgct tgatgaagaa
    24721 tgccgacatt tcgcgggcgt actctaaatc ctcataacga cgttcagtag ggaaggtgtg
    24781 tttattcaac cactccagca attgttcacc ataggccccc accatttcac tttgcggata
    24841 atggatatgt gtatcgacaa agcccggtac tatcagtttg ccgcgatagt cgcgcacgcg
    24901 aatagtgtca ggaatttgat gctttccgtc ttcccattcg ccaaaccatt ccacttttcc
    24961 ctgtttaatg agtaataaac catcctcaat aaaccgcagc gcagaggcaa tctcttccgg
    25021 gttatcgacc gtacgggtga catcaataaa actgcctcgt accgctttca acgtgtgttc
    25081 tcctgacatc attgactcct ttactgttaa tccatctctt ccgctgaggt aataccaggc
    25141 agaacgtttt cctgtcggta gccaccaggg aggataatat ttaataaaat cgcagttaac
    25201 ccgccagcac aaatagggtt ttcaactagt acataaatag aggctggcaa tattttaaaa
    25261 atttcgggat cgtaggagac gccaagtcct aaacctaaag aagtggcgac aataagtgtt
    25321 tctcggcgct ttaaaccgtt ggtgatgatt atgcgaatcc cagcgatggc aatcatggaa
    25381 aacatcaacg tcattgcgcc tcccagaact gccgagggaa tggtcgtgaa gaagccgcca
    25441 atcatcggaa acaaaccgag gataaccagc attaccgcga tggttcgccc gacataacgt
    25501 gaagcgacgc cagtcatctg aataacccca ttattttgcg caaacgtggt taagggtaat
    25561 gacccgacag cggaggcgat aacagaaacc agaccatctg ccagcacgcc gcctttcagt
    25621 cgggactgat actcttcccc ctgaatgggg cggcgggaaa ccattgccgt ggcggtgata
    25681 tcgccgactg cttccagcac gctaagcaga taaatcgtgc caaccaccag gaactgatgg
    25741 aagctaaaac taaagccgta tttgaacgga tgcgggatgg tgattaacgg caaattgcgc
    25801 atactgctga aatccaccat gcccaggcat aacgatgcga tatagccgac acatagccca
    25861 atggcgatcc ctcccatgcg tagcaacgga ctgcgacagc agttaaagcc gatcaccaca
    25921 attaaaacca ataaaccaac gccgagatgt tcgtaattgc cgaacgtacc gctgcttttg
    25981 gctgcaaatc ctccaccaaa atcgataatg ccgactttaa tcaggcttaa gccgatcatc
    26041 agtaccacaa taccgctgac ggtaggcgta ataacccgac gtaaataggg caagataaat
    26101 gaagatccga caaccagaaa tgcgccaacg aaggagacgc cgagaagcga cgacatgatt
    26161 aactcttcgt gaaaaccgtc gcttttcatg ctgctgccca gcgcaatcat gaccgtaaca
    26221 aatgaaaaat tgactgactg aattgagagt aggccagaac cgacgatgcc gtagcggttt
    26281 acttgtaacc aggtaccaat accagaggcg atcatcgcca tagaaacaag ataggcggtt
    26341 gtttcagcgg aaagctgtaa ggccgcaccc acgattaacg cgggggttac catcggaacg
    26401 aaaattgcca acagatgggt aatggcacca acgagagcct gatgaaaggg agggcgatcc
    26461 tccagttcaa atataaggtc agaacctgca tggtttatat cagacatccc ttcactcctt
    26521 ttaaagtaaa tttgatcatc tgaattagta aaataaattc ccacgaattt ggggcgcagg
    26581 acgccttgag aacggcttat ctcattttcg cagtcactat ttagtcatcg ttcaaacaga
    26641 gaaacaggcc gccgaagcgg ccatttatta cttatatttt ttccagcgcg gtgagtattt
    26701 tctccggtgt gaaatgccat tcgcgtaacc agatgccgca ggcatcgtga attgcggtag
    26761 caatcgccgg agctgcgcca tttacaccaa tttccgagat cgatttcgcc ccgaacgggc
    26821 cgactttatc gtcgctcggc accaacacag cgcggaaatc acgcggaatg tcaccaattt
    26881 tcggtgcgcc gtaactgcgt aaatcacgcg ttaacgggtg accttcggcg ttataaatga
    26941 tctcttcgct catactgtgg ccgatagcac gcagggtggc accgtagatt tgtcccaacg
    27001 ctaactctgg attgaccggt gtaccgcagt ccagcaaggc gtagaattta tccaggcgga
    27061 tttcacccgt acgcgtgttg acggcaactt cagcgaagtt tgcgccatac gggaaagcga
    27121 aatcaggcgt gatataactg ccagttccca ccagtgaacc aaagccggtg ccggtttcgc
    27181 ctttatgggc aatatccccg aaactaactt cgcctttctt gccgcgcacg acgcccggcg
    27241 ttgctagttg aacatctgcc actggctcac ccaacatttg cgcgccgtgg aacagaattt
    27301 tctcccgcag attttccgct gccaaacgcg ccgcgttacc cgagaagcaa gtaccggacg
    27361 aggcatatgc gcctttatca aacaacgcat gatcggtatc accggagata acatgcacgt
    27421 cctgcggtgg gcagtgcagc acttctgctg ccagtttcgt cactacagta tccagaccag
    27481 taccaatatc cgcaccgcca gaatgaacga taaaggtgcc gtccgattcc agtttgatca
    27541 tgcagttagc ctgatcgata tccgggatac ccgatttctg catgataatg gcgacgccgc
    27601 gaccgatgtg ccagtcacta ttttgcggtt tcggtgaaga ccattggatc atctcgcgac
    27661 cctgacgcag gatctcttcc agtgcgcagc tggcggcgga aggaacggag gtcggcgctt
    27721 taccttcacc gattgcaccg agaattttca gctcttgccc ttcgtgtacc cggttacgtt
    27781 cgataatttc cagttggtcg atctgtaact gttcagccag ttccgctaat gccatggtga
    27841 tagcgaagtt acctttcggt gcgccataac cctgataagc accatttggg caaatgttgc
    27901 tgtagtaggt ggtgacctgg aaatcgacgt tatcgcacgg atataacggc agcgacagcg
    27961 ccggtccgtt acacggtacg gtgagtgagt ggttgccgta agggccagtg ttggcgcgga
    28021 aatccatctt cactgccgtc aggcgaccat cttttttcgc tcccagtttg acggtgactt
    28081 tcgcgacgtg acgagaggtg ttagcaataa actcttcttc acgggtgtag cggaacagta
    28141 ccggacgccc ggtcacgcag gttgcccatg cgcacacttc ttccagcagg atgtcctgtt
    28201 tggagccaaa accgccgcca actcgctctt taatgacatg aactttatgc tgtttcatgc
    28261 ctacgaggcg cgcgacctgg cggcgtaagt gccatggtac ctgggtggag gcgtggataa
    28321 ccagacgatc gccgtccatc cgggtaaagc agatatgtgt ttcagtcggg cactgctgtg
    28381 cctgcgttga gttataggtt cgctcaatga tcacatcggc atcggcaaag cctttgtcca
    28441 tatcgccaat atgaccatga atactggcgg cgatattttt gcgagggcga gaaccgatcg
    28501 ggaagttgat gatcatatgc tcgccgcgct gggctgcatg gctgttatcg tcttccagag
    28561 tatctggcgc accagcaaca tacaccaccg gttcatcgtg cacgacaggc gcatcttccg
    28621 ccattgcttc gtcgatcgac attaccggct taagcacttc atattcaacg tcgatgagct
    28681 tcaatgcttc gagcgcaatt tcttcacttt ctgcgacgac cgcagcaaca cgatcgccga
    28741 cgtgacgcat tttcttgccg aacatacggc ggtcaagcgg tgacggttcc ggtgcgctct
    28801 gaccacccgg ggtatagtag atatccgggc aattcaggtg agtaataacg tgaacgacgc
    28861 ccggtaaggc ttcagctttg ctgacatcca gatgagtaat cagtgcgtga gcgtgtgggc
    28921 tacgtaacat tttaatgacg caggcgtcag ccgttacgcg gtcttcaaca tagcagggtt
    28981 tcgcctgcac cattttcgcg gcatcagttt taggataatg cttgccaatg acttcaaggt
    29041 cgtcacggaa agtcggagcg atatcaatgg tggcctgcgg attattttta cgtgcaaccg
    29101 ccagttcaat gacctgatag tattgttgcc agcctgcatc gcggctgaac aaaccagaaa
    29161 gcgcgtcgtc gatctcttcg cgggtaggtg cggcgatgcg atcgagaaga tcggtgataa
    29221 tcagagctgc agctggatcg ttataaccag actgcaccac gccaacatca accattgcct
    29281 gttgaaccag acttaactcg ttccatttgc ccaaagattc tgcggtacga atatctgcct
    29341 tctctaactg tgcggcaata agcaaggacg cgttaacgat attaccgtta aagattattg
    29401 cgtcagaacc ggcaaacccg aagccatcat cactgttgcg tacagagtgc attcccatgt
    29461 taaacaacag cttttgcacg ttttcgcctg gattaacggt tagctcctga ggcgcgccat
    29521 ttaaagtaaa gtggatgatc atacggcttc ctccccagcc tgttggcagt cggcatacag
    29581 gtcggctacc agaactcccg tgatatagcg tttataggcc acgctgcccc gcaaatcttc
    29641 ctgcgggaaa atggcgttgg cgacagcctg ttcaagtgca ttgccttcca gattttgttt
    29701 ttcgacatca tgcagacgca gtgctttact ggcgacgcca tccagcgcaa ttcgcatacc
    29761 gtcgtggtct gtcattgcaa cggctgccgt cacgacggtt aaacctgcct gagaacggct
    29821 aattttgcgg gtcgcacagg tgcgatacgg atctttaatg ataatttcgg ttagcaggcg
    29881 atcgcatggg caggccaggt agtcctcgat tgacagcgtt tcgccgttgc caaaaaccag
    29941 ttcagcatcc agtgccaaca ggacgggaag cagcaccgac tcttcctggc gggcggcgat
    30001 ttcaccacca atggtcgact gattacgaac atggcgtgag taaacaaaac cgagggcttc
    30061 acgcagcgct gcaggaataa atcgcgcatc acgcagtggc tgcaagcgag acattgcgcc
    30121 aatccgcagt gcaccgttat cccagtcaac ccagtccagt tccagatcct gtaaggaaat
    30181 ggcaatcttt ttatcggtac gggttggtgt agcattgagt ttgctgcccc cggcgaacca
    30241 gacggcttca tcctggtagc ggcgcttcag ttccagcgcc tgttcgacag agtcgggcct
    30301 gaaaaattgt tcaatcatct tctttcctta tttaaccctc ttccctgcgg gaagagggca
    30361 tctgtcattt atgccagcgc atccatccga cgccacatac tggcagcggc ttttctggct
    30421 tgtgcataaa tggaatcgca atcgaagtta aactgacggt cttcatagac catcacacca
    30481 ttgaccatca cgctgtgaac gctgcctgag cccataccga aagcgatatg cccggcgata
    30541 ttgtctgcca gcagcggcgt cggtgagttg taatcgcaaa tggttaaatc agctttgtaa
    30601 ccggcttcca gaagtccaaa tttcgcgcca aagttgcggc tcatcagttc gttaccgtta
    30661 gtcagggctt tggcaaaact gtcaggccac agcggaccac ccgcatcgcg atgtttaaag
    30721 aaggcaaatt tcatctcttc aaacatgtcc gaaccaatgc cgtccgttcc caacgccaga
    30781 ttgcggatgt cgctaaggtg atggttgtag ccgacatggt tgttcatgtt tgaacgggcg
    30841 ttatgcacca ggaacgcatc gcgctgattg agtagggcga tgtcatcttt cgacaagtac
    30901 agcccatgag cgaccagcgt tttgctatcg atgagatcga attgcgctag tcgtgccagc
    30961 aggtctttgc cgtaccagtg gtgactgtag gaaacgtcgt aaaggtcttc cgcagcgtga
    31021 atatgcaaac cacggcctgt ggctttcacg gcttcacgta gcatctccag accggcatct
    31081 ggcacggtaa acggcgcgtg agcaccgata tgtgcttcca ccagatacgg ctcggttgct
    31141 gctttcttcg cctcatcaat ctgacgggcg aagcggatgt tttcttctac accttcctgc
    31201 aactctttga tgccgttgtt acggtcagta gtttcaaaac aggtcatcgc gcgcaggcca
    31261 acttttaaaa atgcgtcgcg caatgtggag agcgacccgc cgatatacgc cggagaggca
    31321 tggtgatcga taaccgatgt acatccgctc ttaatcgctt ccagggaaca aatcagcccg
    31381 ctgtaataga gcgactcttc atcaagggcg cgatcgagcc gccaccagag atttttcagc
    31441 gttgagatga aatccgggca gggggcgatg tttgccataa ttccgcggga aagccccgag
    31501 taaaaatggt tgtgcgagca gacaattccc ggcatcacaa tccgaccatg catctctttg
    31561 tagctggcat cggggtagcg ttgcgtcagg gcatcgccga tagcgacaat cacatcattt
    31621 tcgatagcga tatcaacgcc ttcctgcact ttcgccgggt gtaactgcac tgcggtgaca
    31681 ttcttcagaa tcaacatgat tacacctcca cgcggcccag cagataatga tgatgctggt
    31741 ggacatggct gatgatgcgg cacatatcgt tcagctccgg tggtacgttg ttgaactgac
    31801 cttcgctgtc gatgtttaac acccagcttt ggttattcag tcgtactcgt acccggcaat
    31861 cttccacaag gaagcctggg ttgctgctgt tatcaaagtc ttgcgccagg ctgaaaacgg
    31921 tgattttgtc tttgtatggt ttaccgttcc acggacagaa ctgagcgcag ttgccgcatt
    31981 cgttacagta agcgtcgagg tgcagcgtct ggaatctatt ctggaagcct gggaccgcaa
    32041 tggagacgtt ggcgcggttc gggcagacat ccacacactt gctgcaaacg tagttacatt
    32101 cgaggcagcg agcggcttcc tgggcgacaa acgcgtcacg atcgtcactg ttcaccagag
    32161 tgatagagat atcgccttta cgttgataga tttccgccgg attgacgttg ttccagtatt
    32221 tatcgttctg gtgggaacgg atattttccc gactaaggat ggcatcggtc gcccgacgcg
    32281 cggttccgac agcagcgaca atggaggatg gtccgcgctg tacgtcgccg atcataaaga
    32341 cgtcagtcag acgagtttcg ccattgtggt cgacgtctgg ccagccgttt ttgtccagcg
    32401 gcacgcccat cgcattcagg gcttcagtat cctgctgttc accaatggcg gtgatcaggc
    32461 tgtctacatg cagtgttact gtttcgttgg tttcaaccgg acggcgacgg cctttctcat
    32521 cgggttcgcc aagcgacata acgcgcaagg ttaaggtgcc atcagcatcg aaacgttccg
    32581 gattattcag gaaacggaac tccacgccgt cgtgcaacgc ttcttcatac tcttcgcgcc
    32641 atgcgggcat ctcttgcagt gaacgacggt aaacgatcgt tgctttttct acgcctggaa
    32701 cgcgtaacgc cgcacgagcg cagtccattg cggtgttacc cgccccgaca acgaccacat
    32761 gtttgcccag cttgagcgct gtacccttgt tgtattcacg caggaagggg agtgatttcc
    32821 aaacattttg gttgtcaccc gccagtttca caccgctatt tttatcagtg ccggtggcaa
    32881 tcagaacata gtggaagccc tgatttttta actgttcaac ggtcaaatcg ggtgagcagc
    32941 catactcaaa tttcacgccg tgatcggcaa caaaatcgat atcgtgctga attaactcag
    33001 ccggaattcg gaactgagga atgatatttt tcaccacgcc gcccgcattg gcttcgcgtt
    33061 caaacagcgt aaccggatgg cccgctctgg caaggaagta acctgctgcc agacccgccg
    33121 gacctgcacc aatcacggca accggatggc gtgaaccaga accggctggt ttgtgccagc
    33181 gttgcttata ttcatcccaa cctttttcca gcgcgacttt tttcagttcg cggatattca
    33241 gcgcactgtc gtaatccagg cgggtacagt tgtattggca ctggtgatcg caaatatgac
    33301 cggtaatggc gggcagagcg ttgcgttggt agatgagttc cagcgcgtcg gcatagcggt
    33361 gttcgccaag cagacggatg tattccggaa tatcttgctt gatagcgcag gcagtaacac
    33421 agggggcaac gtagcaatcg gtcagcggca ggtcttctgc cacttcaata cgctcttctg
    33481 gcttccagtg tttctgggtg tattccatgg ttaacgcatc tgctgccagt ctgttcagtc
    33541 gttcgacgtc aacatggtca agtccccagg cgtcggagcc ttccagctcg cgcatgcagg
    33601 cacttaagcg caaatagccg ccaggtttca gcaggtcggt tgccatagta atagggcgaa
    33661 tacctgtatc aaaaatatcg cggatagtca gctgactggc accaccggaa taagaaatgg
    33721 gcagtttgcc gtcaaaggcg cgagagagaa ctgctgcaac attgatggag agcgggaaca
    33781 gcgcacggcc tgacatatac atctcttcac caggcagtgc acctttattg ttgatggtgc
    33841 cgagagtgtt agtcagtttt acgccaaagc cgagtgattt ttcttttgcc agtgccatca
    33901 ggcgttccag catttccagt gcttgcgtca gcttgaggtc gtgatcaaat gactcttctt
    33961 ttaagcctat gtaaccgaaa ccgcagacat cgaggatctc acgaacacgc gcgtatccca
    34021 gtaaggtcgg gttaagtttc acaaaggtgt tgagcccttt ttcttccagc atgtagcggc
    34081 aaatggcttc gatttcatgc ggaggacagc cgtgcatggt ggagagggtg acgccctgca
    34141 ccatactggt ggggatgcga gcgggtaagg cttgcaagct ttcgcgtttt tcctgcaatc
    34201 cgtggcgggc taaaaatgcg tcatcctgga gtaatttatt cagcgtatcg cgatattgcg
    34261 cgaatttcgg atggtcagat gcgtccatca tattgtcgat gaactgttgc attggcggtt
    34321 gcttaatacc ttcgaggttg taaccgacgc tcatattaaa gatgaacgat ttaccggaat
    34381 cagaaggctg gaacatcgct tcgagaaggt gcagggcaaa ccaggctttg aggtattcat
    34441 cccaggcttt aagcagggta aattcggtag accattcggt gttaaagcac tcgtcttcgg
    34501 catcgataca gggcttttcc agctccaggc ggtcaagaat ttggacggtt tttagttcga
    34561 tgaagcgtcc gccagtcagc caggaagtga caatattttg cgcgagctgc gtgtgcgggc
    34621 cagcggcagg gccgacggga gtggcacagg tttcaccgaa gacgctaaca gttttacctt
    34681 ttacaggtga gtaaaactgt tgctcgggaa taccaaagat tgagcgttgt tgttggtatt
    34741 catcaaatat gcgcgtcaaa agttcctcaa acggaatggg acgcataata tcccccataa
    34801 ccctctcctt agcttataca gatatcattg agtgaccggt gttgagaaat ccggtggttt
    34861 agggggatag agcaacaatc gcaccactct tgccaggttt ggcgggaaat ataaaaaaat
    34921 gtgaggcggc tcgaaactta aaggcttttt gtgaatgaac tcacaggtgc caaatgagaa
    34981 ttaaaatcac tcctgaaaat tattattgtc agcccattta tcactttttc tcttttctca
    35041 aaatcagtga tt
//
LOCUS       DAZGJW010000037        34461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZGJW010000037 DAZGJW010000000
VERSION     DAZGJW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.037"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(120..196)
                     /locus_tag="ACHKIK_001821"
     tRNA            complement(120..196)
                     /locus_tag="ACHKIK_001821"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(160..162),aa:Arg,seq:acg)
     gene            complement(260..336)
                     /locus_tag="ACHKIK_001822"
     tRNA            complement(260..336)
                     /locus_tag="ACHKIK_001822"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:Arg,seq:acg)
     gene            complement(534..610)
                     /locus_tag="ACHKIK_001823"
     tRNA            complement(534..610)
                     /locus_tag="ACHKIK_001823"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(574..576),aa:Arg,seq:acg)
     gene            complement(614..706)
                     /locus_tag="ACHKIK_001824"
     tRNA            complement(614..706)
                     /locus_tag="ACHKIK_001824"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(670..672),aa:Ser,seq:gct)
     gene            complement(1022..1207)
                     /gene="csrA"
                     /locus_tag="ACHKIK_001825"
     CDS             complement(1022..1207)
                     /gene="csrA"
                     /locus_tag="ACHKIK_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO9083008.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1442..4072)
                     /gene="alaS"
                     /locus_tag="ACHKIK_001826"
     CDS             complement(1442..4072)
                     /gene="alaS"
                     /locus_tag="ACHKIK_001826"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO9083009.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(4200..4700)
                     /gene="recX"
                     /locus_tag="ACHKIK_001827"
     CDS             complement(4200..4700)
                     /gene="recX"
                     /locus_tag="ACHKIK_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO9083010.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLVAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4943..6004)
                     /gene="recA"
                     /locus_tag="ACHKIK_001828"
     CDS             complement(4943..6004)
                     /gene="recA"
                     /locus_tag="ACHKIK_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO9083011.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(6084..6581)
                     /gene="pncC"
                     /locus_tag="ACHKIK_001829"
     CDS             complement(6084..6581)
                     /gene="pncC"
                     /locus_tag="ACHKIK_001829"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO9083012.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6726..7811)
                     /gene="mltB"
                     /locus_tag="ACHKIK_001830"
     CDS             complement(6726..7811)
                     /gene="mltB"
                     /locus_tag="ACHKIK_001830"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO9083013.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYVQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            8067..8630
                     /gene="srlA"
                     /locus_tag="ACHKIK_001831"
                     /gene_synonym="gutA"
     CDS             8067..8630
                     /gene="srlA"
                     /locus_tag="ACHKIK_001831"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO9083014.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8627..9586
                     /gene="srlE"
                     /locus_tag="ACHKIK_001832"
                     /gene_synonym="gutE"
     CDS             8627..9586
                     /gene="srlE"
                     /locus_tag="ACHKIK_001832"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO9083015.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFREGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9597..9968
                     /gene="srlB"
                     /locus_tag="ACHKIK_001833"
                     /gene_synonym="gutB"
     CDS             9597..9968
                     /gene="srlB"
                     /locus_tag="ACHKIK_001833"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO9083016.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9972..10751
                     /gene="srlD"
                     /locus_tag="ACHKIK_001834"
     CDS             9972..10751
                     /gene="srlD"
                     /locus_tag="ACHKIK_001834"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO9083017.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10856..11215
                     /gene="gutM"
                     /locus_tag="ACHKIK_001835"
     CDS             10856..11215
                     /gene="gutM"
                     /locus_tag="ACHKIK_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO9083018.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            11282..12055
                     /gene="srlR"
                     /locus_tag="ACHKIK_001836"
                     /gene_synonym="gutR"
     CDS             11282..12055
                     /gene="srlR"
                     /locus_tag="ACHKIK_001836"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO9083019.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            12048..13013
                     /gene="gutQ"
                     /locus_tag="ACHKIK_001837"
     CDS             12048..13013
                     /gene="gutQ"
                     /locus_tag="ACHKIK_001837"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO9083020.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(13010..14524)
                     /gene="norR"
                     /locus_tag="ACHKIK_001838"
     CDS             complement(13010..14524)
                     /gene="norR"
                     /locus_tag="ACHKIK_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO9083021.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14711..16150
                     /gene="norV"
                     /locus_tag="ACHKIK_001839"
     CDS             14711..16150
                     /gene="norV"
                     /locus_tag="ACHKIK_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO9083022.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            16147..17280
                     /gene="norW"
                     /locus_tag="ACHKIK_001840"
     CDS             16147..17280
                     /gene="norW"
                     /locus_tag="ACHKIK_001840"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO9083023.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(17506..19758)
                     /gene="hypF"
                     /locus_tag="ACHKIK_001841"
     CDS             complement(17506..19758)
                     /gene="hypF"
                     /locus_tag="ACHKIK_001841"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO9083024.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAEFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGKIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19911..20438)
                     /gene="hydN"
                     /locus_tag="ACHKIK_001842"
     CDS             complement(19911..20438)
                     /gene="hydN"
                     /locus_tag="ACHKIK_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO9083025.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGVGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20587..21597)
                     /gene="ascG"
                     /locus_tag="ACHKIK_001843"
     CDS             complement(20587..21597)
                     /gene="ascG"
                     /locus_tag="ACHKIK_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO9083026.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGRWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLICRDSLIAL
                     SR"
     gene            21857..23314
                     /gene="ascF"
                     /locus_tag="ACHKIK_001844"
     CDS             21857..23314
                     /gene="ascF"
                     /locus_tag="ACHKIK_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO9083027.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVMLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            23323..24747
                     /gene="ascB"
                     /locus_tag="ACHKIK_001845"
     CDS             23323..24747
                     /gene="ascB"
                     /locus_tag="ACHKIK_001845"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO9083028.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSITWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24861..25280)
                     /locus_tag="ACHKIK_001846"
     CDS             complement(24861..25280)
                     /locus_tag="ACHKIK_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9083029.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(25277..25588)
                     /locus_tag="ACHKIK_001847"
     CDS             complement(25277..25588)
                     /locus_tag="ACHKIK_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO9083030.1"
                     /translation="MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTA
                     FPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25762..26505)
                     /locus_tag="ACHKIK_001848"
     CDS             complement(25762..26505)
                     /locus_tag="ACHKIK_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083031.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(26548..27018)
                     /gene="hycI"
                     /locus_tag="ACHKIK_001849"
     CDS             complement(26548..27018)
                     /gene="hycI"
                     /locus_tag="ACHKIK_001849"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO9083032.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(27011..27421)
                     /gene="hycH"
                     /locus_tag="ACHKIK_001850"
     CDS             complement(27011..27421)
                     /gene="hycH"
                     /locus_tag="ACHKIK_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO9083033.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(27418..28185)
                     /gene="hycG"
                     /locus_tag="ACHKIK_001851"
     CDS             complement(27418..28185)
                     /gene="hycG"
                     /locus_tag="ACHKIK_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO9083034.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(28185..28727)
                     /gene="hycF"
                     /locus_tag="ACHKIK_001852"
     CDS             complement(28185..28727)
                     /gene="hycF"
                     /locus_tag="ACHKIK_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO9083035.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28737..30446)
                     /gene="hycE"
                     /locus_tag="ACHKIK_001853"
     CDS             complement(28737..30446)
                     /gene="hycE"
                     /locus_tag="ACHKIK_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO9083036.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(30464..31387)
                     /gene="hycD"
                     /locus_tag="ACHKIK_001854"
     CDS             complement(30464..31387)
                     /gene="hycD"
                     /locus_tag="ACHKIK_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO9083037.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(31390..33216)
                     /gene="hycC"
                     /locus_tag="ACHKIK_001855"
     CDS             complement(31390..33216)
                     /gene="hycC"
                     /locus_tag="ACHKIK_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO9083038.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMRVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(33213..33824)
                     /gene="hycB"
                     /locus_tag="ACHKIK_001856"
     CDS             complement(33213..33824)
                     /gene="hycB"
                     /locus_tag="ACHKIK_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO9083039.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33949..34410)
                     /gene="hycA"
                     /locus_tag="ACHKIK_001857"
     CDS             complement(33949..34410)
                     /gene="hycA"
                     /locus_tag="ACHKIK_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO9083040.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
BASE COUNT         8097 a         9597 c         8864 g         7903 t
ORIGIN      
        1 attactcgcc ttgcggctcg cccttcgggc cgttgctaaa gcaacgttat catccctggt
       61 gcctgcggtt aattccgcta cctcttcgat accttcattg ctgaagattt cggagaatat
      121 ggtgcatccg ggaggattcg aacctccgac cgctcggttc gtagccgagt actctatcca
      181 gctgagctac ggatgcatcg ggaaaattta ttttactgct gatattgata ccgctactaa
      241 agccatatca agtaagagat ggtgcatccg ggaggattcg aacctccgac cgctcggttc
      301 gtagccgagt actctatcca gctgagctac ggatgcatcg ggaaacttac tttactgcag
      361 attttttgat accgctacta aagccgtatc aagtaagaga tggtgcatcc gggaggatta
      421 ctcgccttgc ggctcgccct tcgggccgtt gctaaagcaa cgttatcctc cctggtgcct
      481 gcggttatac cgccacctct tccatacctt catcgctgaa agtacgtaga atatggtgca
      541 tccgggagga ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta tccagctgag
      601 ctacggatgc aaatggcggt gaggcgggga ttcgaacccc ggatgcagct tttgaccgca
      661 tactccctta gcaggggagc gccttcagcc tctcggccac ctcaccacac gcctcttacg
      721 agtgcttcga agaacttgtt tatcgctcat cgtcgctgcg tggcgcacat attactttct
      781 gagacttata agtcaaacaa tttttcctac acttttatcg tttgcacact tcacgttcaa
      841 ttagtctgta aaaacgtcaa aaagagtgtt ttatcaacag aaaaatggag gtctgacaga
      901 tagtagtaat gcaaaaaaat ggagacttaa gttgaatgaa cgggagtaaa gcgaaaagac
      961 tatagagtga aggagaaatt ttgagggtgc gtctcaccga taaagatgag acgcggaaag
     1021 attagtaact ggactgctgg gatttttcag cctggatacg ctggtagatc tcttcacggt
     1081 gaacagaaac ttccttcggg gcatttacgc caatacgtac ctggttgccc tttaccccta
     1141 aaactgtcac ggtgacctca tccccaatca tgagggtctc accaactcga cgagtcagaa
     1201 tcagcattct ttgctccttg aaagattaaa agagtcgggt ctctctgtat cccggcatta
     1261 tccatcatat aacgccaaaa agtaagcaat gacaaacaca ttacatgtaa gcagtcatgg
     1321 cattacattc tgttaaacct aagtttagcc gatatacaca acttcaacct gactttatcg
     1381 ttgtcgatag cgttaatgcg aatgccgtga agcgagtcca cggcattgcc tgacgcttat
     1441 attattgcaa tttcgcgctg acccagcctt tcacactggc taacgctgca ggtaaggccg
     1501 cagcatccgt accaccggct tgcgccatgt caggacgtcc accacccttg ccgcccacct
     1561 gctgagcgac cataccaatc agttcccctg ctttcacacg atctgtgacg tccttagata
     1621 cgcctgcaat cagagaaacc ttaccttcgg ctaccgttgc cagcacgata attgtcgacc
     1681 ccagctgatt ttttaaatcg tcaaccatgg tacgcaacat tttcggctca acaccgctaa
     1741 gctcgctaac caacagctta acaccattaa catcaattgc cttactggaa agatttgcgc
     1801 tctcctgtgc ggcagcttgt tctttaagct gttgtagttc tttttctaac tgacgcgtac
     1861 gttccagtac tgagcgcact ttatccgcca gattattgct atcgcctttc agcagatgcg
     1921 cgacttcgct taagcgatcg ctgtctgcat gaacggtggt gatagcgcct tctccggtta
     1981 ccgcttcgat acgacgaacg cctgcagcag tacccgattc agagatgatg cggaacagac
     2041 caatatcacc agtgcggctg gcgtgagtac cgccacacag ctcggtggag aaatcgccca
     2101 tgctcagcac gcgtacacgc tcatcatact tctcgccgaa cagcgccatc gcacctttcg
     2161 ctttcgccgc ttcgagatcc atgatgttgg tttcgatcgg caaattgcga cgaatctgtg
     2221 cgttcaccag gtcttcgacc gcacgaatct cttccggttt catcgcttcg ttgtgtgaga
     2281 agtcgaagcg cagcaccttg tcgttaacca gtgaaccttt ctgcgataca tgagtcccca
     2341 gaacctggcg cagcgcagcg tgcatcaggt gcgttgcgga gtgattcaga cgaatacggg
     2401 cgcgacgagc ctcatcaaca tcagcctgca cagcgtcgcc cactttcaga gaacccgcag
     2461 caagtttacc gatgtgacca atcgcctggc cgtatttctg cgtatcttcc accgcgaagg
     2521 agaagttagc gcctttcagt tcgcctttat caccaacctg accgccggat tccgcataga
     2581 atggtgtttg atccagcacg accacagctt cctggcctgc attgattgca tcaaccgctt
     2641 taccatcaac aaacagcgcg gtcactttgc cgttcagttc cagatggtca tagcctttaa
     2701 attcagatgc actatcaaca cggatcattg cgttgtaatc ggcaccaaag ccgctggctt
     2761 cgcgcgcgcg acgacgctgc tcttccattg ctgcttcaaa accagcttcg tcaactttga
     2821 tgttgcgctc acgacaaaca tcagccgtca ggtcaaccgg gaagccatag gtgtcgtaca
     2881 gacggaaagc cgtttcacca tccagcgtat caccagaaag ttttgccagc tcttcatcca
     2941 gcaacgccag accgcgctcc agagtacggg caaactgctc ttcttcagtc ttcagcacct
     3001 gctcaacctg cgcctgctgg cgtttcaggt cttcacccgc agagcccata acgtcgatca
     3061 gcggaccaac cagtttgtag aagaaggttt ctttcgcgcc gagcatattg ccgtgacgca
     3121 ctgcgcgacg aatgatacga cgcagtacat aaccacggtt ttcattggac ggcattacgc
     3181 catccgcgat caggaacgca caagaacgaa tgtggtcagc gattacgcgc agcgatttat
     3241 tgctcagatc ggtcgcgcca gtgactttcg ctaccgcctg gatcaacgtg cggaacaggt
     3301 cgatgtcata gttagagtta acgtgttgca gcaccgcagc aatacgctcc agacccatac
     3361 cggtatctac agacggcttc ggcagcggtt ccatcgtgcc atctgcctgg cggttgaact
     3421 gcatgaagac gatgttccag atctcaatgt agcggtcgcc gtcttcttcc ggacttcccg
     3481 gaggaccacc ccaaatgtgg tcgccgtgat cgtagaagat ttcggtgcac gggccgcacg
     3541 gaccagtgtc acccatctgc cagaagttgt cagatgcgta tggcgcacct ttgttatcgc
     3601 cgatgcgaat aatacgttcg cgcgggatcc cgacttcttt ttcccagatt tcgtaggctt
     3661 cgtcgtcgct ttcatagacg gtaacccaca gacgctcttt cggcagggca aaccattttt
     3721 cgctggtcag cagttcccat gcaaactgaa tggcatcgtg tttgaaatag tcgccgaagc
     3781 tgaagttgcc cagcatttcg aagaaggtat ggtgacgcgc ggtgtaaccg acgttttcca
     3841 ggtcgttgtg tttaccaccc gcacgcacgc agcgttggga agtggtagcg cgggaataat
     3901 tacgcttgtc gagcccaagg aacacatcct tgaactggtt catcccggcg ttggtaaaca
     3961 acaaagttgg gtcgttatgg ggtaccaggg agctgctggc aactacctga tgtcccttac
     4021 tatggaaaaa gtcgagaaac gcctgacgga tctcagcggt gctcttgctc ataattatcc
     4081 tgaaatcaag ctaacgaaat atcgccacca gctccagcgt gtcttaaccg ccgggctggt
     4141 aactgaaaag tgggaataag ataagttttc ttgactggga agtaaaatac cgtatgcgtt
     4201 cagtcggcaa aatttcgcca aatatcctgg atatcttcca tcagatagcc acgatagagc
     4261 agaaaacgct ggatcttaac tttttctgaa aagacagttg gcaaaggttc accatatttt
     4321 cgcgtcgcct gatcgcgcgc cagtgcgcac cagtcgatgt cacattcacg catcgctttt
     4381 tctgtcgctt cgcgggaaat acctttctga ttcagttcct gacgaatacg tgcaggtcca
     4441 taacctttgc ggctacggct ggcgataaag cgcgcaacaa atcggctgtc atcgagatag
     4501 ccatgttcat ggcaccaggc aataacgcgc tcgtaatctt ctgccgtagc atcaatctct
     4561 tctgggccat ttttgcccat aatcggtgcc acgagtttac gtcgcagttc ttgctcactg
     4621 tgatcgcgca ccgccagaat gcgtaccgca cgatccaaca ggcgagcata tgccgggcga
     4681 cgggacgttg attctgtcat ggcatatcct tacaacttaa aaaagcaaaa gggccgcaga
     4741 tgcgacccag gttttgctga taaacagatt taatagtcaa tgagtgtgca gaatgttccc
     4801 gcaggcccaa ttcgttagag gatcgaggtg agagtaattt ttcacgccag tgctggcctg
     4861 ttcccctccc cctaaccctc tccccaaagg ggcgagggga ccgtccgtgc acacattatc
     4921 tattatcatc aataacgaac gattaaaaat cttcgttagt ttctgctacg ccttcgctgt
     4981 catctacaga gaaatccggc gttgagttcg ggttgctcag cagcaactca cgtactttct
     5041 tctcgatctc tttcgcggtt tccgggttat ctttcagcca ggcagttgca ttcgctttac
     5101 cctgaccgat cttctcacct ttgtagctgt accacgcgcc tgctttctcg atcagcttct
     5161 cttttacgcc caggtcaacc agttcgccgt agaagttgat accttcgccg tagaggatct
     5221 gaaattcagc ctgtttaaac ggtgcagcga ttttgttctt caccactttc acgcgggttt
     5281 cgctacccac cacgttttcg ccctctttca ccgcgccgat acgacggatg tcgagacgaa
     5341 cagaggcgta gaatttcagc gcgttaccac cggtagtggt ttccgggtta ccgaacatca
     5401 caccaatttt catacggatc tggttgatga agatcagcag cgtgttggac tgcttcaggt
     5461 tacccgccag cttacgcatc gcctggctca tcatacgtgc cgcaaggccc atgtgagagt
     5521 cgccgatttc gccttcgatt tccgctttcg gcgtcagtgc cgccacggag tcaacgacga
     5581 taacgtctac tgcgccagaa cgcgccaggg cgtcacagat ttccagtgcc tgctcgccgg
     5641 tgtccggctg ggagcacagc aggttatcga tatcgacgcc cagtttacgt gcgtagattg
     5701 ggtccagcgc gtgttcagca tcgataaacg cacaggtttt accttcacgc tgcgctgcgg
     5761 cgatcacctg cagcgtcagc gtggttttac cggaagattc cggtccgtag atttcgacga
     5821 tacggcccat cggcagacca cctgccccaa gcgcgatatc cagtgaaagc gaaccggtag
     5881 agatggtttc cacatccatg gaacggtctt cacccaggcg catgatggag cctttaccaa
     5941 attgtttctc aatctggccc agtgctgccg ccaacgcttt ctgtttgttt tcgtcgatag
     6001 ccatttttac tcctgtcatg ccgggtaata ccggatagtc aatatgttct gttgaagcaa
     6061 ttatactgta tgctcataca gtatcaagtg ttttgtagaa attgttgcca caaggtctgc
     6121 aatgcatacg cagtagcctg acgacgcacc gcatcacggt caccgctgaa gcattcccgc
     6181 cgggtaatgc cttcaccgcg ggcagtggca aaagcaaacc agacggtgcc gacaggcttc
     6241 tcttcactgc cgccatccgg cccggcgata ccactaatag acacggcata atcagcacga
     6301 gccgctttca gtgcacctat cgccatttcc accacgacgg gttcactcac cgcgccatgc
     6361 tgcgccagcg tctcttcgcg tacgccgatc atctgcgctt tggcttcgtt actgtaggtg
     6421 acaaatccgc gttcaaacca ggcggagcta ccggcaatat cggtaatcac tttcgctacc
     6481 caaccaccgg tacaagactc ggcagttgtt acggttgcgc cacgggcttt cagcgcctgc
     6541 ccaacctgtt cacttaactg catcagttca ctgtcagtca tcacaacctc tgtcagtcaa
     6601 aaatttatgc cggacaagat agcactttcg tcaggaagat ggtgttggcg ttgcaatttt
     6661 acgaggagga ttcagaaaaa agctgattag ccagagggaa gctcacgccc ccctcttgca
     6721 aatagttact gcacgcgcgc cagcgccacg gcttgtccta actgccagac cgccattgcg
     6781 taatgggtgc tgtggttata acgggtaatg gtgtagaagt tcggcagacc gtaccagtac
     6841 tggtaaccgg taccgacatc cagacgcagc aggctggctt gttgatggtt gcccagcggc
     6901 tgctgtggcg ttaaacctgc cgtggcaagc tgcgaaatgc tgtacctggt tttgaagcca
     6961 tttggcaagc ctggagcctg accgtttgcc attaccgcga cctgatcgcc tttcacccag
     7021 ccgtgcgctt tgaaatagtt cgccacgcta ccgatcgcat caaccggatc ccacaggttg
     7081 atatgcccgt cgccgctgaa atctaccgca tattgtttgt aagacgacgg cataaactgt
     7141 acgtagccca tcgccccggc aaaggaacct ttcagattga gcggatcgtc ctgctcgtcg
     7201 cgcgccatca acaggaaggt ttccagctcg ccagagaaat actccgcgcg gcgtgggtag
     7261 ttaaatgaca gcgttgccag cgcatcgagg atgcgagttt tccccatcac gcgcccccag
     7321 cgggtttcaa cgccgataat cccgacgata atttccggcg gtacgccata cacctgccac
     7381 gcgcgattca acgcatcttc atactgattc cagaaaacca caccgttctg cacgttgtcc
     7441 ggcgtaataa attttttgcg ataacggagc catgcgccgt tcggacctga tggcggtttc
     7501 accgatgtgg ttggtgcctg gttatccatc agccgcagta ccgaatccag acgcttcgcc
     7561 tgggagagaa tttcctgcaa ctgctgacga tcgaaaccgt gtttgttcac cattttgtcg
     7621 atgaactgct gggcattcgg gttattagcg aaatcgccgc ccatctgcat cacattgtgc
     7681 tgcggctcaa gcaggaagcc gccagacggc gttccggtgg tcgtttcagt ctcagtaggt
     7741 tttggcttgc tgctacaggc ggcaagcaac acaaaaaggg gaagcaatgt tacataacga
     7801 cgcttgaaca tgaggggtcc atttaacaga ttcaaccagg ggcaagtatg gtaaagcatc
     7861 acgccccaca caaggaagcg gtagtcactg cccgatacgg actttacata actcaactca
     7921 ttcccctcgc tatcctttta ctcaaacttt caaattaaaa tatttatctt tcattttgag
     7981 atcaaaataa cacttttaaa tctttcaatc tgattagatt aggttgccgt ttggtaataa
     8041 aacaataaat cctgaaggag agaacaatga tagaaaccat tactcatggt gcagagtggt
     8101 ttatcgggct gttccaaaag ggcggagagg tgtttaccgg gatggtgacc ggcattcttc
     8161 cgctgttgat tagcctgctg gttatcatga acgcactgat taattttatc ggtcagcatc
     8221 gtattgaacg ttttgctcaa cgttgcgccg gtaaccctgt ttcccgttac ctactgttac
     8281 cgtgcattgg cacgtttgtc ttttgcaatc cgatgacctt aagcctgggt cgctttatgc
     8341 cggaaaagta caaacccagc tactacgcgg cggcctctta tagctgccac tcaatgaatg
     8401 gcctcttccc ccatatcaac cctggcgaac tgtttgttta tcttggcatt gccagcggtc
     8461 tgacaacgct gaacctgcca cttggcccac tggcggtgag ttatctgctg gttggtctgg
     8521 tcaccaattt cttccgcggc tgggtgaccg atctgaccac cgccattttt gagaaaaaga
     8581 tgggcattca acttgaacaa aaagttcacc tggcaggagc aacatcatga cgcatattcg
     8641 gatcgaaaaa ggaacgggtg gctggggcgg cccgcttgag ctgaaagcca cgccgggaaa
     8701 aaaaatcgtc tatatcaccg ccggtacccg gcctgcgatt gttgacaaac tggcacagct
     8761 tactggctgg caggctattg acggatttag agaaggtgaa cccgcggagg cggaaattgg
     8821 tgtcgcggta atcgactgtg gcggcacatt acgctgcggc atctatccga aacgacgtat
     8881 tcccaccatt aatatccact cgacgggcaa gtccggtccg ctggcgcagt acattgtgga
     8941 agatatttat gtctctggcg taaaagaaga aaacatcact gtagtgggtg atgcgacacc
     9001 acaaccctct tccgtgggcc gtgactatga caccagtaag aaaatcaccg aacaaagcga
     9061 tggtttactg gcgaaggtgg gaatgggcat ggggtccgcc gttgcggtgc tgtttcaatc
     9121 tggtcgtgac accatcgaca ctgtattaaa aaccattctg ccgtttatgg cattcgtctc
     9181 ggcgctcatt ggcatcatta tggcttctgg ccttggtgac tggattgccc acggtcttgc
     9241 tccgctggcg agccatccac tgggtctggt catgctggcg ctcatctgct ccttcccact
     9301 gctttcacct ttcctcggcc caggcgcagt tatcgcacag gttatcggcg tattgattgg
     9361 cgtgcagatt ggtctcggca atattccgcc gcatctggct ttaccggcac tgtttgccat
     9421 caacgcgcag gcggcctgcg acttcatccc ggtcggtttg tcgctggcgg aagcccgtca
     9481 ggacacggtt cgcgtcggtg tcccttctgt actggtgagc cgctttttaa ccggcgcacc
     9541 gactgtactg atcgcctggt ttgtctccgg ttttatctat caatagaggc tgaaacatga
     9601 ccgttattta tcagaccacc atcacccgta tcggcgcgag tgccactgac gccctcagcg
     9661 accagatgct catcaccttt cgtgaaggcg cgccagcgga cctcgaagag tattgcttca
     9721 ttcattgcca cggcgagttg aaaggtgcac tccatcccgg tttgcaattt acgctcgggc
     9781 agcatcgcta tccggtgacc gctgttggca gcgtggcgga agacaacctt cgcgaactgg
     9841 gtcatgtcac cctgcgcttc gatggtttaa acgaagcgga atttccgggc actgtccatg
     9901 tggcaggccc tgtacccgac gatatcgcgc cgggatcggt tttgaagttt gaatctgtta
     9961 aggagtaaaa aatgaatcag gttgccgttg tcatcggtgg tgggcaaacc ttaggcgcgt
    10021 tcctgtgcca cggtctggct gccgaggggt atcgcgtcgc ggttgtcgat attcagagcg
    10081 acaaagccgc aaatgtggca caagaaatta acgccgaata tggtgaaagt atggcgtacg
    10141 gttttggtgc tgacgccact agcgagcaaa gcgttctggc gctctctcgt ggggtagatg
    10201 aaatctttgg tcgcgtggat ttgctggtct atagcgccgg aatagcaaaa gcagccttta
    10261 tcagcgactt ccagctcggc gattttgacc gttcgctaca agtgaatctg gtgggttatt
    10321 tcctgtgtgc ccgtgaattt tcgcgtttga tgatccgcga cgggattcag ggtcgcatta
    10381 ttcagatcaa ctcgaaatcc ggcaaagtgg gcagcaaaca caactctggc tacagcgcag
    10441 cgaaatttgg tggcgtcggg ctgactcaat cactggcgct ggatctggcg gagtacggca
    10501 ttacggtgca ttcactgatg ctcggtaacc tgctgaaatc gccgatgttc cagtcactgt
    10561 tgccacaata cgcgaccaag ctgggtatca aaccggatca agtcgagcag tattacatcg
    10621 acaaagtacc gctcaaacgc ggctgcgatt atcaagatgt gctgaatatg ctgctgttct
    10681 acgccagtcc taaggcgtcg tactgcaccg gacagtcgat caatgtcacc ggcggtcagg
    10741 tgatgttctg atcaacagcg gagatccatt aaggatctcc gtgagactat agaatgcctg
    10801 atgcgctacg ctcatcaggc atacaggact tccgccacta cattaaggaa aagttatggt
    10861 atccgcactc atcaccgtcg ccgttatcgc ctggtgtgcg caactggcct taggcggctg
    10921 gcaaatttct cgttttaacc gtgccttcga cacactatgc cagcaagggc gggttggcgt
    10981 gggccgttcc agcgggcgct ttaaaccgcg ggtcgtggtc gccatcgcgc tggacgatca
    11041 gcagcgcatc gtcgacacct tgtttatgaa aggactgacc gtcttcgccc gaccgcaaaa
    11101 aattcccgca attaccggta tgcatgcggg tgatttacag cccgatgtga tctttcccca
    11161 tgatccacta tcacagaatg ctctatcatt ggcgcttaaa ctgaaacgtg gataatttcg
    11221 ttgtgaatgt tacttgcttg cgaagttatc attttgaaac ctaaatcagg taatcacgcc
    11281 catgaaacct cgtcagcgtc aggccgccat tctggagtat ctgcaaaagc agggtaaatg
    11341 ctcggttgaa gaattggcgc aatactttga caccacaggc acaaccattc gcaaagatct
    11401 ggtcattctg gaacatgccg gaaccgtcat tcgtacttat ggcggagtgg tgttgaataa
    11461 agaggaatcc gatccgccta tcgatcataa aacactcatc aacacccaca agaaagagct
    11521 gattgcagaa gctgccgtta gttttatcca tgatggcgat tcgatcattc ttgatgctgg
    11581 cagtaccgtt ttgcagatgg ttcccctgct ctcgcgcttt aataacatca cggtgatgac
    11641 caacagcctg catatcgtca atgcgctatc cgaactggat aacgaacaaa ctatcctgat
    11701 gccaggcgga acgtttcgta aaaaatcggc ctcatttcac ggacagctgg cagagaatgc
    11761 cttcgaacat ttcagtttcg ataaattgtt tatgggcacc gacggcatcg atctcaatgc
    11821 gggcgtaacc accttcaacg aggtttatac cgtcagtaag gcaatgtgca atgccgcgcg
    11881 cgaagtgatt ttgatggcgg actcatcaaa gtttggccgt aaaagcccca acgtagtttg
    11941 cagtcttgaa agcgtcgata agctgattac cgacgcaggt atcgatccgg cgtttcgtca
    12001 ggcgctggaa gagaaaggga tcgatgtgat cataaccgga gagagcaatg agtgaagcac
    12061 tactgaacgc gggacgtcag acgttaatgc tggagttgca ggaagcaagc cgtttaccgg
    12121 aacgtctggg cgatgatttt gttcgcgccg ccaatatcat cctgcactgc gaaggcaaag
    12181 tggtggtttc gggaattggc aaatcgggcc acattggtaa gaaaatcgcc gcaacacttg
    12241 ccagtaccgg cacaccggct ttttttgtcc atccggcaga agcactgcac ggcgatctgg
    12301 ggatgattga aagccgcgat gtgatgctgt ttatctctta ctccggtggc gcgaaggaac
    12361 tggatctgat tattccgcgt ctggaagata aatctatcgc gctgctggcg atgaccggca
    12421 aaccgacgtc accgctgggc ctggcggcaa aagcggtgct ggatatctcc gtagaacgcg
    12481 aagcctgccc gatgcacctt gcgccgacct ccagcaccgt caataccctg atgatgggtg
    12541 acgcgctggc gatggcggtc atgcaggcgc gcggatttaa tgaagaagat tttgcccgct
    12601 cccacccagc cggggcactg ggcgctcgct tgctgaataa agtgcatcat ctgatgcgcc
    12661 gtgacgatgc catcccacag gtggcgttaa ccgccagcgt gatggatgcg atgctggaac
    12721 tcagccgcac cggtctgggg ctggtggcgg tatgtgacgc tcaacaacag gtacaaggcg
    12781 tctttaccga cggcgattta cgtcgctggc tggttggcgg cggcgcactc accacgccag
    12841 ttaatgaagc gatgacgaca ggcggcacca cattacaggc acaaagtcgc gccatcgacg
    12901 ccaaagagat cctgatgaag cgcaaaatca ctgccgcacc ggtggtggat gaaaacggca
    12961 aactcaccgg cgcaattaac ctgcaggatt tctatcaggc cgggattatt taatccttca
    13021 atcccagacg tttcgccagc cgatgcaggt tggcgacgtc ggtttccagc atccgcgcgc
    13081 aggcagccca gttgtgatga ttttgtgcca gtgcctggcg aatggtttca cgctggaacg
    13141 cttctgtcgc ttcacgcagg ttttgcttaa caacgggcac cgccgccgct tctggcggcg
    13201 gcaacgtcac ctcaggaaaa gcaaaatgtt gtgcctcaag aatcacttca tcgccgctgc
    13261 gagtggctct cgccagcact accgcccgat gaatagcatg ttccagttcg cgcacgttcc
    13321 ccggaaaacg atagtgttgc agtaaatttc gcgctccggc acttaatacc acgcgggaga
    13381 gccccagccg caaacgacac tgctcgcaga aataccccgc cagcagaatg acatcatcgc
    13441 cccgctcacg cagcggcggc accgaaagtg gaaacacgct caggcgatga aacaaatcgg
    13501 cgcggaatcg ccctgccagc acctcttcgc gtaagtcgcg gttagtcgcc gccagcacgc
    13561 gcacatcgac ccgcaaacta cggtcatcgc caacgcgctg aatatcgcca tactgcaaca
    13621 ccctcagcag cttggcctgc aatgccaacg acaactcgcc gatctcatcg agaaacagag
    13681 tgccgttatc cgccatttca aacttcccgc tgcggttact gatagcgcca gtaaacgctc
    13741 ctttcacatg cccgaacaac tcactttccg ccacactttc cggcagtgcg gcacagttga
    13801 gatagaccag cggattcacc gcccgtggcg aggcttcatg aatcgctttc gccaccagct
    13861 ccttaccggt tcccgtctca ccgctgatca gaacgttgag atcggacgcc gccacaatct
    13921 caatctcttt tttcaattgc gtcatgcctg gcgacaagcc aatcatctgc gtctgtttca
    13981 ccgcttcaaa cggcgcggca tcgcccggca gcatattctg gctttccagt tgttcaatca
    14041 gcaacgcatt gcttaacgct cccgccgcca gcgcggcaat cagccgtaac tcttcgtcgc
    14101 tgaaaacatc gaactgatcg ggctgcatcc cgtcgagcgt cagtgcgccg atcaggtttt
    14161 gcccggcaaa caatggcaga ccaacgcagg cgtgaacctt cagactctcc tgcccaggaa
    14221 tcaaaccgtc atagggatcg ggcaattcgc tgtctgcagg aaagcgcacc acgtccccgg
    14281 cgcgggcaat cgcttcaagc cgtggatgcc cttccagcgc aaagcgtcta ccgagtacat
    14341 cctttgccag accgtcgatg gcaagcggaa taaactgccg cgaatcgtaa cgtagcaacg
    14401 cagacgcatc gcactccagc acctgacgta gcgtggtgat caggcgctga aaacgatcct
    14461 gatgaccaat cccacgctgc aattcgatgg cgatattcgc cagcacatca acggaaaaac
    14521 tcatctttgc ctcactgtca atttgactat agatattgtc atatcgacca tttgattgat
    14581 agtcattttg actactcatt aatgagcata attttattta tagagtaaaa acaatcagat
    14641 aaaaaactgg cacgcaatct gcaattagca agacatcttt ttagaacacg ctgaataaat
    14701 tgaggttgct atgtctattg tggtgaaaaa taacattcat tgggttggtc aacgtgactg
    14761 ggaagtgcgt gattttcacg gcacggaata taaaacgctg cgcggcagca gttacaacag
    14821 ctacctcatc cgcgaagaaa aaaacgtgct gatcgacacc gtcgaccata aattcagccg
    14881 cgaatttgtg cagaacctgc gtaatgaaat cgatctggcg gatatcgatt acatcgtgat
    14941 taaccatgca gaagaggacc acgctggggc gctgaccgaa ctgatggcgc aaattcccga
    15001 tacgccgatc tactgtactg ccaacgctat cgactcgata aatggtcatc accatcatcc
    15061 ggagtggaat tttaatgtgg tgaaaactgg cgacacgctg gatatcggca acggcaaaca
    15121 gctcattttt gtcgaaacac caatgctgca ctggccggac agcatgatga cttacctgac
    15181 aggcgacgcg gtgctgttca gtaacgatgc tttcggtcag cactactgcg acgagcatct
    15241 gttcaacgat gaagtggatc agacggagct tttcgagcag tgccagcgtt actacgccaa
    15301 tatcctgacg ccgttcagcc gcctggtaac gccgaaaatt accgagatcc tgggctttaa
    15361 cttaccagtc gatatgatag cgacctccca cggcgtggtc tggcgcgata acccgacgca
    15421 aattgtcgag ctgtacctga agtgggccgc tgattatcag gaagacagaa tcaccatttt
    15481 ctacgacacc atgtcgaata acacccgcat gatggctgac gctatcgccc agggaattgc
    15541 ggaaaccgac ccacgcgtgg cggtgaaaat tttcaacgtc gcccgaagcg ataaaaacga
    15601 aatcctgacc aatgtcttcc gctcaaaagg cgtgctggtc ggcacttcga cgatgaataa
    15661 cgtgatgatg ccgaaaatcg ccgggctggt ggaggagatg actggtttac gcttccgtaa
    15721 caaacgcgcc agtgctttcg gctctcacgg ctggagcggc ggtgcggtgg atcgtctttc
    15781 cacgcgcctg caggatgcgg gtttcgaaat gtcgcttagc ctgaaagcca aatggcgacc
    15841 agaccaggac gctctggagt tatgtcgtga acacggtcgc gaaatcgccc gtcagtgggc
    15901 gctcgcgccg ctgccgcaga gcacggtgaa tacggtggtt aaagaagaaa cctctgccac
    15961 cacgacggct gacctcggcc cacggatgca gtgcagcgtc tgccagtgga tttacgatcc
    16021 ggcaaaaggc gagccaatgc aggacgttgc gccaggaacg ccgtggagtg aagttccgga
    16081 taacttcctc tgcccggaat gctccctcgg caaagacgtc tttgaagaac tggcatcgga
    16141 ggcaaaatga gtaacggcat tgtgatcatt ggttcgggct tcgccgcccg ccaactggtg
    16201 aaaaatattc gcaaacagga cgccactatt ccattaaccc tgattgccgc cgacagcatg
    16261 gatgagtaca acaaacctga cctcagccat gttatcagtc aggggcaacg tgccgacgac
    16321 cttacccgcc agacggcggg tgaatttgcc gagcagttta atctgcgcct gtttccgcac
    16381 acctgggtga cggatatcga tgccgaagcc cgtgtggtga aaagccagaa taatcagtgg
    16441 caatacgaca agctagtgct ggcaaccggt gccagcgcct ttgtcccgcc agtgcctggg
    16501 cgtgagttaa tgctgacgtt aaatagtcag caagagtatc gcgcctgtga aacgcaactg
    16561 cgggatgccc gacgcgtgtt gattgtcggt ggtggcttga ttggtagcga actggcgatg
    16621 gatttttgtc gtgcaggcaa agcggtcacg ctaatcgaca acgctgccag tattctggcg
    16681 tcattaatgc caccggaagt aagcagccgc ttgcagcatc ggttgacgga gatgggcgtt
    16741 catctgttgt tgaaatctca gttgcagggg ctggaaaaaa cagattctgg cattctggca
    16801 acgctggacc gccagcgcag tatcgaagtg gatgcggtaa ttgccgccac cggactgcgc
    16861 ccggaaaccg ccctggcacg acgcgccggg ctgacgatta atcgcggcgt ttgcgtcgat
    16921 agttatctgc aaaccagtaa tgccgatatt tacgcgctgg gcgattgcgc ggaaattaac
    16981 ggtcaggtat tgccgttcct ccagccgatt caacttagcg cgatggtgct ggcaaaaaat
    17041 cttctcggca ataacacgcc actgaaacta ccggcgatgc tggtgaaaat caaaacgccg
    17101 gaattaccgc tgcatctggc aggcgaaacc cagcgtcagg atttacgctg gcagattaat
    17161 accgaacgcc agggaatggt ggcgcgcggc gttgacgatg ctgaccagct tcgcgccttt
    17221 gtggtcagtg aggatcggat gaaagaggca tttggattgt tgaaaacgtt gccgatgtag
    17281 gtgggctact gtgcctaaaa tgtcggatgc gacgctggcg cgtcttatcc aacctacggg
    17341 gagcgcatgt gttggtcaca tactgcgttc atgtcgcatc cacctactgt gcccaaaatg
    17401 tcggatgcga cgctggcgcg tcttatccga cctacgggga cgcatgtgta ggccggataa
    17461 ggcgtttacg ccgcatccgg caatggtgtc caaatgcaac acgttttatc cgttctggac
    17521 ttcacccgct aaccaacgcg ccgcagcaat aaccccctgc cccagagaca aaccgccatc
    17581 acccgccggt aaactctgtg gaaagagcaa tgtgaaatca gcgagataat gcgccagacg
    17641 tgcacgcagc aaacggttat gaataacccc gccgctaaat accagcgtag tgataccacg
    17701 catcgtggcc tgctcacgca tcaacgcggc aaaaccctgc gccagcgcat catgaaacgc
    17761 ccacgcgcgt tgattaaccg gtgcctgcca gttcagccac tgctgccaga aagtggcgag
    17821 atccagttga ttgtccacca gcggcattgt caccggatgc gtcactccgt ggcacgaagc
    17881 tgcgagtgcc tccagagcac aagccgcttc accttcataa cttaacgtgg ctggcgcaca
    17941 gcccagtgcc gccgccacgg catcgaacaa ccgcccacac gatgacgcca gcggcgcgtt
    18001 aattccacgc tcaatggccc gcgccagcac gctccagttt tgctgttgca cacttgctgt
    18061 ttcagaataa ttctgccact ccggcacaaa gcgcaggcac tgcgccagca ggtttcgcca
    18121 cggctgcttc gccgccaaat cgccacccgg aagcgccact gcaggcaagc cgcccaggtg
    18181 ctcacattcg cgatagttca cccgcagaca ctcgccgccc cacaaagcgc cgttctcccc
    18241 cataccgata ccatcgagcg tcaaagcaat gacatccccg ccatccagcg gccactgatg
    18301 ctctgccaga cacgccgctg cgtgggcatg atgatgcagc accgtttgcg tcggcatatt
    18361 catttcgctc gcccactggc tggagacata gcccggatgc acgtcatgca caacgtattg
    18421 cggagtgaaa tcgtagatgt tttgcatcag gcgtaacgct tcgcgccact gcatctggat
    18481 gccatcgtca cttaaatcgc ccagatgctg actcaacacc gcttgttcac cgcgcaccag
    18541 gcagaaggtg tttttcagat ccgcgccgag acacagcaca ggcggaacat ttttaaagcc
    18601 cggaggcaaa gccagcgcat ccggcacata cccccgcgaa cggcgcagca tttcgccgct
    18661 ttcgcgcacc accgaatcat ccatccgctg cacgatgtcg cggttatgta tcaagaatcc
    18721 gtcggcaatg ccctgcaaat ccgccagcgc ctgttcgttg ctgatagctg gtggtttacc
    18781 gctcaggttg ccggaggtca tcaccagcgg gcattgcagt tcctgtaaca gcaaatgctg
    18841 gagcgggttc gcaggcaaca ttaccccgac ttcgttaagg tcaggggcga tatcatcaca
    18901 aagctcagga acgtattttt tatccaccag cacaatcggc gcggcgggcg tggtaagcaa
    18961 ctggcgcgca gcgtctggta aaccgtcagc cactggcaac atgactgcca gcggtttcgc
    19021 cgggcggtgt ttgcgctccc gcagtgtcgc caccgcgtta ctgttacgtg catcgcaggc
    19081 aagatgaaat ccgccaatcc ctttgatggc gacaattttg cccattttta actgtgcgat
    19141 agctgcctgt aatgccgcct cttgttccgc atgttcacca tgacttaccc attcaagatg
    19201 cgggccacac tccgggcagg ccaccggctg ggcgtggaag cgacgatcga gcgggtcgcg
    19261 gtactcttta tcacaggctg gacacagcgg aaactccgcc attacggtaa acgggcggtc
    19321 gtaaggcatg gcgcgaataa tggtgaaacg cgggccgcag tgggtacagt tgataaacgg
    19381 ataacgataa cgccgttcgc ctggggtatt catttcggca aggcaagcag ggcaagtagc
    19441 ggcatccggg acaatttgcg tattcatggc acctcccgcg ctctggcgga tagtgaactc
    19501 ggtgggcagt tgtgaccaga taaacggctc acgctcgacg ctatcaatac gcgccagtgg
    19561 cggacagtgc tgatgcaatt gaacaagaaa cgtttccggg tcttccagca gccggacttc
    19621 tacgccatcg ccgtcattac agacatcgcc gtgaagattt aattgctgtg ccagctgcca
    19681 gacaaacgga cgaaaaccga cgccctgcac tttgccacga atacgcagtt ggacaccgca
    19741 agatgtgttt tttgccattg agttattccc gccatcatga attgcgtaac ccgtccagcc
    19801 ggacacgaca gcgtcgcatc cggcagtcac aggtcggcga taccgcccgc ttcgtattct
    19861 acgaatattt ccgggaattc ctttgatgcc agaacagttc tgtaagattt ttagaacatc
    19921 agcgccgtac ggcggcgttt ttctgcgctc agttgttcaa gtttattacg atcgacacaa
    19981 attagcgcat gggtcgggca agccgccata cacgccgggc cgtcttcacg atggttgcac
    20041 aggtcgcatt tattggcttc agctttgtca gcccgtacat tcagccccac gccgctgttg
    20101 cgaatcaccg gacgtaccac cacttccatc gcgccatacg ggcaagccac cacgcaggtt
    20161 ttgcaaccaa tacaacgttc ctgcatcaca tgaacaaacc ctttatcacg gctgatagca
    20221 ccattcgggc agacgttagc gcacggtgca tcttcacact gacggcaaac tgtcgccgtg
    20281 gaaatgttca cacctttaat gacatggata cgcggtaaaa atgtttccgg ggtcagcgat
    20341 gcacagtcct gattttcctg atgagaaacc acgcacgcca cttcacaggt acggcaacca
    20401 atacatttac tcgcgtcagc aatgatgaaa cggttcatca aattctccag caatgacagt
    20461 taatgcgccg atacattcac aaatcatgcc agtttttaat ttactgttat ttaaggaaat
    20521 taatttctgt aatgcaggaa aaacgatgtc atcgacacta gtgacgatga catgtgatga
    20581 caatgtttat cgcgaaaggg caatgagtga gtcgcggcag atcaattttc cgctgaaggt
    20641 tttcggcggt gagaaatccc cgccatcgag cataaaaatc agccgtccaa taatttcctg
    20701 aatcatctca gtcaccggaa tttttacgct ggagagcgcc ggaacgatat agggggcaat
    20761 tgcgatatca tcgaatccga taactgacac ctgctctggc accgctacgc cgcgctcgtg
    20821 caacgctttc atcgcaccta tcgccatatc gtcgttactg gcaactaacg cgctaaattt
    20881 agccccacgt tcgagcaaca tttctacccc ttcggccccg ctggcaggcg tccatctacc
    20941 gttagcgata agtttttcat tgagcgcaat accgtgctgc gccagcgcgt ctttatatcc
    21001 ggcaagacgt tcaacgctgg tgggagaatc catcgaaccg gtaaggaaag caatctcctg
    21061 atgtccggcg tttatcaact ctgccacggc gttaaaactg gtctgtttat gatcgcacca
    21121 gacgctatgg ctgctgtttt tgcgcaggcg gcgattaagc accattatcg gctgactgtg
    21181 cgcgtcaatg atgtcatcga tctcatccac gcttaaaaaa cgcgggtaaa tcataatggc
    21241 gtcgcagcgc aaatccagca gatactgaat cgcctggcgc tcttcttcgg cgctgtgttt
    21301 accatctgcc aatagcaact gccgcccttt ctcttccgcc attcgcgcgg catgaaacag
    21361 taattcacta aaataaatgc cgtggtaaag cgtgttggtc actaccagcc ccagcgtctg
    21421 agtactcttc gccgacaaat tgcgcgccag caagtttggg cggtaaccgc tctcttctac
    21481 cgcctgaaac acgcgatctt tagtctcctg gctgacgtag ccattacctg aaagcacgcg
    21541 ggaaacggtc gcttttgaaa ccccggcgcg cttcgccact tccagcatcg tcgtcatcat
    21601 tttcatccct ttacacgcaa tcaacgcagt gtactgcacc gtttgccgat tgtccttgcg
    21661 caatcggcgg gaaaaatatt caggtgaccg gtttcacaaa tataaaaaat gaacaattaa
    21721 ctctcttgct tatttagtga caactattca tgattttgtg aaaccggttt cctaattccg
    21781 tttcagcatc ggcatttttc cgttacgtcg actgataaca actacatcta ccctactgat
    21841 aacaggataa aatccgatgg ccaaaaatta tgcggcgctg gcacgctcgg tgatagcggc
    21901 actgggcggc gttgataaca tctcggcggt cacgcactgt atgacgcgtt tgcgctttgt
    21961 tatcaaagat gaccagctta tcgacagccc gacgttaaaa accatccccg gcgtgctcgg
    22021 cgtggtacgt agtgacaacc agtgtcaggt gattatcggc aataccgttt cacaagcctt
    22081 tcaggaagtc gtcagcctac tgccgggaga tatgcagccc gcacagcccg tgggtaaacc
    22141 caaactcacg ctgcgtcgca ttggtgcagg gatcctcgat gcgctgatcg gcaccatgtc
    22201 accgctgatc ccggcgatta tcggcggatc gatggtcaaa ctgctggcga tgatcctcga
    22261 gatgagcggc gtgctgacaa aaggatcgcc gaccttaacc attctgaatg tgattggtga
    22321 cggtgctttc ttcttcctgc cgctgatggt cgcggcatct gccgccatca aatttaaaac
    22381 caatatgtcg ctggcgattg ccatcgcggg tgtgctggta catccgagtt ttattgaact
    22441 gatggcgaaa gcggcccagg gtgaacatgt cgaatttgcc ctgattccgg tcaccgcggt
    22501 gaaatacacc tacacagtga tcccggcgct ggtgatgacc tggtgcctgt catatatcga
    22561 acgctgggtg gatcgcatta caccggcggt gaccaaaaac ttcctcaagc caatgctgat
    22621 tgtgttgatt gccgcaccgc tggcaatcct gctgattggc ccgattggta tctggatcgg
    22681 tagcgccatt tcggcgctgg tttacaccat tcatggttat ctgggctggc tttcagtcgc
    22741 cattatgggc gcgctgtggc ctctgctggt aatgaccggg atgcaccgcg tctttacgcc
    22801 aaccatcatt cagaccattg ccgaaaccgg caaagaaggg atggtcatgc cgtcagagat
    22861 cggcgctaac ctgtcgctgg gcggttcatc actggcggtg gcgtggaaaa cgaaaaaccc
    22921 ggaactgcgc cagacggcgc tggctgcggc ggcatcagcc attatggcgg ggatttccga
    22981 accggcgtta tacggcgtgg cgatccgcct gaaacgtccg cttatcgcca gtcttatcag
    23041 cggctttatt tgcggcgcgg ttgccggtat ggcggggctt gccagccact caatggcagc
    23101 gccggggcta tttaccagcg tgcagttctt cgatccggcg aatccaatga gcatcgtctg
    23161 ggtgttcgcg gtcatggcgc tggcggtcat gctgtcgttt atcctcacac tgttgctcgg
    23221 ctttgaggat attcctgttg aggaagcgac tgccgaggcg cgaaagcatc agagcgtaca
    23281 accgaccgtc gccaaagaag taagtcttaa ttgaggatga aaatgtcagt atttccagaa
    23341 ggttttttat ggggcggcgc gcttgccgcc aaccagtctg aaggtgcgtt ccgtgaaggt
    23401 ggcaaagggc tgaccactgt cgatatgatc ccacacggcg agcatcgaat ggcggtgaaa
    23461 ctggggctgg aaaaacgttt tcagttgcgc gatgacgagt tttatcccag ccatgaggcg
    23521 acggattttt atcatcgtta taaagaagat atcgccctga tggcagagat gggattcaag
    23581 gttttccgta cctcaattac ctggagccgt ctctttccgc agggcgatga aatcacgccc
    23641 aatcagcagg gcattgcttt ttatcgttct gtctttgaag agtgtaaaaa gtacggtatc
    23701 gaaccgctgg tcacgttgtg ccacttcgat gtgccgatgc atctggtcac cgaatatggc
    23761 tcctggcgta accgcaagct ggtggagttt ttcagccgct acgcccggac ctgctttgaa
    23821 gcatttgatg gtctggtgaa atactggctt accttcaatg aaatcaacat tatgttgcat
    23881 agcccgttct ccggcgcggg tctggtgttt gaagaaggtg aaaatcagga tcaggtgaaa
    23941 tatcaggccg cgcatcacca gctggttgcc agtgcgctag ccaccaaaat cgcccatgag
    24001 gttaacccgc aaaatcaggt gggctgtatg ctggcgggcg gtaacttcta cccttacagt
    24061 tgcaagccgg aagatgtctg ggcggcgctg gagaaagacc gggaaaacct gttttttatc
    24121 gatgtgcagg cgcggggcgc gtatccggct tactctgccc gcgtattccg cgaaaaaggg
    24181 gtaaccatcg acaaagcacc gggcgatgat gaaatcctga aaaacaccgt cgattttgtc
    24241 tctttcagct attacgcctc gcgctgcgcc tcggcggaga tgaacgccaa caatagcagt
    24301 gcggcgaacg tagtgaaatc gctgcgtaac ccgtatctgc aagtgagcga ctggggctgg
    24361 ggtattgatc cactcggtct gcgtatcacc atgaacatga tgtacgaccg ttatcagaag
    24421 ccgctgtttc tggtggaaaa cggcctcggc gcaaaagatg aacttgctgc caatggcgag
    24481 attaacgatg actatcgcat cagctattta cgcgaacata tccgcgcaat gggcgaagcg
    24541 attgcagatg gcattccgct gatgggctac accacatggg gctgtattga tttagtttcc
    24601 gcctctacgg gtgaaatgag caaacgctac ggttttgtct acgtagaccg tgacgacgca
    24661 ggcaacggca cgctgacgcg cacacgtaag aaatcgttct ggtggtataa aaaagtgatt
    24721 gccagtaatg gtgaagattt agagtaagca acagtgccgg atgcggcgtg aacgccttat
    24781 ccggcctaca tgtcgcacga accaggaagg cccgataagc gcagcgcatc gggcaatctg
    24841 gggtttggca tcagtcaaaa tcaaagaaac cattccggct taacgccaaa tcgggctgaa
    24901 agtgccttaa tgtgggatat cgtcagggat cgttgtccag acaaaatctg actgaccagg
    24961 gatttagagc cgatttcgtt cttcaaatcc gcataggtca gattatgttg atcaatcagc
    25021 gtgcgaagta acgccacgcc aacaggcatt gccgcaacgg ctttgtcgaa ctctgcaaac
    25081 ttttcgttgt tgttttcata ctctgcaatg cggctcgcca gaaagtcgat aagtggattg
    25141 gtgtcatctt cttcaatcaa atattcgacc agttccagtg cttcacgata atcatcttct
    25201 gagtcactgc ctcccagaaa ggggactgca ttgaccagtt ctcttgttgc tttaacagcg
    25261 cgtgcagcat tagcagtcat tctttgttct ctctgtagta acgtgtcagt ttgtcatact
    25321 ctgcgtgatt cgttatgtgc ttcacaaaga accgcttatt aacaaagttt atgtaggcaa
    25381 taaccctaag attgttcccg ccgacatcca gaacgtacca tttgttgcga tatctaaaat
    25441 tgtcgaggtt gggaaacgcc gttcgcattt cttctggtgt agaaaaatcc gtttccttaa
    25501 taacccgata caatgcctga tgagccagag cgtcgttagg atatttacgc gcagactctt
    25561 caaatggcgc tttagaaatg atgtgcattt ttaagtttta acttccttat agtaagtaaa
    25621 cagccacatc tgcgatgttc tatccttatg tgttcggaaa ctatagctca tcaaatccaa
    25681 gttcacaata tgtaaacact tgtgcgcgat ataattaccc ccaaaatcct catagccact
    25741 ctgcaaaaag tcagttctgg ttcacccctc aagcacatcc ccctttatca agcaacgctg
    25801 cgtctgcgcc agatcttccg tttgcgggtc gcgcccggcg atgggtttga ggacgagttt
    25861 acggttgggg gtataagtaa aacgcgtgag cgcgggatag cttaacttgc caaaccagcc
    25921 gtgaacacgc tgccatttgg cgtcttttac ctgatacaga ctggttatcc agtaaccaga
    25981 attaaactgc atatccagca attgcgtgtg accattgcct tgcaaatcca gcaaatcatc
    26041 tacgccagtt ttgcttgcgg tgtagaatcc cctgggttca aagacacagg gacggccttc
    26101 gcgcgtgaac gtcataagaa ttaaatgtgc ataaggagcc agaccattac cggtacttgg
    26161 taaccagata accagatcct gaatgccgtt acgatcaaga tcggcgcgat agagttgccc
    26221 tttgcccgct atgtttgcca gcatacccac aggcacagtc caggatttac cggcgcggtc
    26281 tttagcggtt aattgccagt aatcttcttc atttggcatc ttctcaagcg tcacgccagc
    26341 aacgcccact ttcctaaaat gccaggtttg ctcggcatca taaggcatgg tcaggagagg
    26401 tttcagcgcc cgcttttgcg cgtattcgct gtcactcatt ggcagatatt cggtaaagct
    26461 ggtggcctgt gcaggcagca ctaagctcaa taaaaaagcg cacatccctg ttctgatgtt
    26521 caattccctg aattccctga tggagaatta ttcttcttcc accgctaact gcgcaaatcc
    26581 accatttcct tcccagcctt ccagtcgttg ataaacggtt tctaccgcat ctttaatcgg
    26641 ctgggtcatc gggtagtaaa agccgacgat atccggctga atgccgagga aaatcacttc
    26701 accaatgtct tcttttaact ggtcgataag gtaattcaac ggcatgttat gggtagtcat
    26761 cataaacatc tcggcgatat catccgggtc gatgatgcgg atttcgccgg ggtttagccc
    26821 catatccgtg gcgtcgacaa tcagcagtcg tgtcgggcgc agttcacgga tggcgacgat
    26881 gtcgttttcc ggtgcactac cgccgtcaat caccacccag ttacctttcg gcgcggcggc
    26941 gcacttttcc gccagcagcg ggccagcgcc atcatcgccc atcatgctat tgccaacaca
    27001 gagtaaaacg tcagtcacgc agtctcctca ccatcaaata gatggcgttt tcctgatgaa
    27061 tatcatgcag catacttaac agcattgtac tccattcctt ctgctgcgaa gtttgcgctg
    27121 cccgggcttt atcgaatgcg ttggccagca tcggcacatg gttaatgtcg atgacgatct
    27181 cgccatattt cggtacgcct tccattttgc ggcgggcttc gctgcccgcc tccagagtgg
    27241 caatccatgc cagatattca tcccatgggc aggtgagcgc cgcttccagg caatcgataa
    27301 ccccaaggtg gtgaccaatc gccaggctgt aatagaccac ctgctgcgcc tcggcgggcg
    27361 tggcatcgtt ctcatcaata aatttacggc tcagttgact gaacaccacc ttttcactca
    27421 tcggatacgc gcctcttcaa caacatgatt cagatggctg acaatctcgt tcagacgcgg
    27481 atcgttttcc gcttccagcc agcgtgcaac ctgttcttcg ccctgcccta actgtgtaag
    27541 gtaatcatcg gcaatctgac gaccgtaacg ataacccgcc aggcgacgtg cttcgcgatc
    27601 cactttcacg cgcagcggct gcaccatatc gccatgcaga atctcagccg gttgttcatc
    27661 ctgctcaccc gggccacggg cgtgaatttt ctgctccagc aggccgagtg ccattgcaaa
    27721 gccgtacagc gtggcggcag gcgttggcgg gcaacccgga atgtagacat ctaccgggac
    27781 gattttatcc gtaccgcccc acacgcagta gagatcgtgg aaaataccac cgctgttacc
    27841 gcaggcaccg taggagatac aaattttcgg gtccggcgcg gactgccacg cacgcagcgc
    27901 aggggatcgc attgcacggg tgaccgcgcc ggtaaacagt aaaatatccg catgacgcgg
    27961 tgaaggaacg actttaatgc cgaagcgttc tgcatcaaac agcggcgata aagtggcgaa
    28021 aatttcgatt tcgcagccgt tgcagccgcc gcagtccacg cggtaaacat aggcagaacg
    28081 tttgattttt ttcagtaacg acgccttcat gctggcgatg gattcatcca ccgtcatcgg
    28141 gaccggaatg ccgttggcgt cacgggggcc taataaattg ctcatcagat ggcctctttc
    28201 atatggcgag tcagttcaat acggtcggac ggcaccaggc atttctggcg cttacattcc
    28261 gggcaagtct caaagctttc gcggtggttt tccgcgcggc tgtcaccatt gtgcttaagc
    28321 agcgcaatgg cgtagtcgat ctctttctgg acggcgaaag gacgattgca gacgcgacag
    28381 ctgcacagca caaaacggga ctgttgcagg aaatcttctt tcttccacac cgccagttcg
    28441 tactcttgcg acagtttgat cgctgccgtc gggcagactt cttcgcagcg tccacagaag
    28501 atgcagcgcc cgagattaaa ctcccacgcc agctcaccgg tggcgaggtc agtttcaacc
    28561 gttaaggcgt ttgacgggca ggcattgacg caggccgcac agccgatgca ctgctgcggg
    28621 ttctgctccg gcttaccacg gaagttttta tcaaccgcaa tcggctccag cggataagac
    28681 gaggtcgccg tgccggtttt gatgactttt ttgataaagg taaacatggc gattccttat
    28741 ttcagcggcg agtttttacg ctcaatgctg taacgctcga gttctttgta cggcaccact
    28801 ttgctctttt tcttacgcac atcgaccacg gtcatgcggt cggtacagga gtagcaaggg
    28861 tccaggctac cgataatcag cggcgcatcg gaaacggtgt taccgcgcag catgtagcgc
    28921 agggtcggcc agttcgcgta ggtcgcggca cggcagcgcc agcggtacag cttctggttg
    28981 tcgccggtca tgctccagtg gatatcgtcg ccgcgcggcg cttcggcaaa gcccagcgcg
    29041 aaacggtgcg gaatgtaggt aaagccttcc accatcagcg gaccgcccgg caggttatcc
    29101 agaccgtagt cgatcatgtt cagcgcggta taaacttcgt tgatacgcac tttcagacgg
    29161 gaaataacgt cgcagccctg ctcgctgtgg acttccattg gcagcaggcc ataaccgaca
    29221 aacgggtgat cggcgcgagt atcgcgggcg tgaccgctgg cgcgaaccat cgggccaacg
    29281 ttactgaaat cgcgggcgat ttccgggtcc agacgaccaa tgccgacggt gcgctgttcc
    29341 atgttcggcg tactcagcag cacatccacc agctcctgca cttcacgacg catctgttgc
    29401 gccagctggc gggtctggat catgtcatct ttcaacagat cgcgacgaat cccgccgatc
    29461 aggttcaggc cgtaggtttt acgcgcaccg gtgaggatct ctgccatttt catggaggtt
    29521 tcacgcacgc ggaagaactg cataaagccg gagtcaaagc cggtaaagtg gcaggcgaga
    29581 ccgaggttga gcagatgcga gtgcaggcgt tccacctcca gcagaatggc gcggatcatc
    29641 tgcgcacgtt ctggcacctg aatacccatc gcgttttcta ccgacgtggt gtaggcggtg
    29701 ctgtgggcaa agccgcagat cccgcacaca cggtcagaga ggaaggtcac ttcgttgtaa
    29761 cccatacggg tttccgccag tttttccatg ccgcgatgaa cgtagaacag acggtagtcg
    29821 gcgtcgataa tgttttcgcc atcgacgaac agacggaagt ggcccggttc gtcagaagtg
    29881 acgtgcagcg gaccaatcgg cacgacgttg tttttcttgt cgcccagttc gttgatgaac
    29941 tcgtaggttt cagcatcggt agtcggtgcc ggacgctgac gataatccat gctgtcttta
    30001 cgcagcggat aaagttcatc cggccagtca tccggcagca ccagacgacg ttcatccggc
    30061 agaccaaccg gaatcaaacc gtacatatcg cgcacttcgc gctcgcccca caccgccgcc
    30121 ggaacgcgcg gcgtcacgga cggatactcc ggtttgttgg cgtcaacttc gacgcgaacc
    30181 gttacccaac acttggtgcc cttctccatc gacagcacgt aataaacggc gtaatgacca
    30241 ttcagtttgc gttcgtcgtt accaaacagc accgacagcc agccaccctg cttgtagtag
    30301 agaaactcca ccacttccgg caggtagttc accttcacgg tcacggtcag ctgatctttg
    30361 gtctgccagg cgtggtccag cacgacgccc ggaaatgcct cattcagcgc ggcgagataa
    30421 tgttgaccta atttttcttc agacatgctc aaactctctt taatcacgcc gccagcaagg
    30481 agacgaacgc taaaaatgca aagccaaacc cagcccaggt aatgcgcgga gtaatatcaa
    30541 gacgcagacg ggccatgctg ttttcgaaca gcgcgataac caggacgccg acgaccagtt
    30601 tgacgatggc aatcaccagc gccagcagca gcccaccggc ggtgaaggtt tccatctgtc
    30661 cccacgggat aaatacgccg acgaacatct gcagcaccac cagctgtttc aggctgatac
    30721 cccatttcat gacgccaaag ccgctgccgc tgtattcaga gagcgggcct tcctgcagct
    30781 cctgctcggc ttccgccagg tcgaacggca gtttgcccat ttcgataaag gtggcgaacg
    30841 cacaggcaca aagcgccagt accagcggga tgctctggct cagcggccag tgataaacgg
    30901 tgtcggtgat gttgctgatg ttggtggaac cggcaacctg tgcggcaacc cacagaccaa
    30961 gcagcagcat cggttcgacc agcacgccaa gcatcgcttc acggctcgcg ccgatagcgg
    31021 taaacgggct accggtatcc agaccagaaa tggcaaagaa gaaacgcgcg atggcaaaga
    31081 gatacagtaa ggtgattaaa tcacccagtt gcggcagcgg agaaccgacg gtcaccaccg
    31141 gcagcgcagt agcgatagtc agcatgacgc ccaccatcac atacggcgtc aggcggaaca
    31201 cccagccgga ggcatccggg ccaacgctct ggcggcccag cagtttgata atgtcacgat
    31261 actcctgcaa cacacccggc ccacgacggt tatgtaagcg ggctcgcgcc acgcgggtaa
    31321 taccggagag cagcggcgca acggcaaata gcaccagcgc ctgaattaac ggatataaaa
    31381 cactcattct caggctcctc gtgaaacaat aatcaccacc agtaccgcca gttcaaccag
    31441 cgccatccgg cggaacagca acgcactccc ctcgcactgc cagcccggca ccagagacac
    31501 cggattcagc catttgcgta gtttcagcac cggcgcaaac gcctgtttca ccggcatggc
    31561 aaaaccgtga gcggtaatca ccattgattt ttcgtggtcg tagccgcaca cccaggccgc
    31621 gccgcgggaa cgcgatggca aacgatcgcc tttgcaaatc gccataatga tgaatggcag
    31681 cagcggacag gcaatcagca gcaatgtgat cattggttga gaaacggtgg tgttagcagg
    31741 ctccagcggc agaggtacag cagcagacag catcggcagc agccacggcg cagcaacacc
    31801 gccaattacg cagcaaatcg ccagtgccac tacgcttacc ctcatcagga gcggcgcaca
    31861 ggtggcgttt tcggcttctt tggtacgcgg cgcgccgagg aacgtgacgc catagacttt
    31921 cgccatacac atcaccgcca gcgcaccggt aattgccaga cccacagcga gcagcggccc
    31981 cagcagacgg gcaacaaacg cgccgctatt gctcagtttg aagaaggatt gatagataac
    32041 ccattccccg gcaaacccgt tcagcggcgg cagcgcagcc attgccatta acccgactaa
    32101 catggcgatg gagataaccg gcattttctt gccaatacca ccgagttttt cgatatcgcg
    32161 atgaccggta cggaaccaga cgctccccgc cccgaggaac agtacgcttt tgaacaggct
    32221 atggttaagc agatggtaca gaccaccgac caggccaaga gcaatcagcg ccggttgttc
    32281 gagcgcgata cccgttacgc cagcgcccag ccccagcagg ataatgccga tattttccag
    32341 ggtgtggtaa gccagcaggc gctggatgtt gtgctccatc agcgcataca gaccaccgac
    32401 aaacgcggtg atcatgccga gcaccagcag cgcgatcccc caccacagcg gtgcattacc
    32461 gcccagcagt gacagggtta aaatgcccag caggccaatt ttcatgacta ccgtagaaaa
    32521 caacgcggca gctggcgcag aggcgttagc atgtgcctgc ggcacccagc cgtgcagcgg
    32581 aataatcccg gcgagcaggc caaagccaat cacaccgagc agccagatat cggaaccgag
    32641 cggcagctgt tgcatacgca tatccagcag gcgcagatcc agcgtgccat aacgctgcca
    32701 cagcagccag caagcaatcg ccagcagcag agtgccaaga cgccccagcg caaaccacag
    32761 tttgccctct ttgctgttgc tagtgaggaa caccgcgcac agggccatga tttcggccat
    32821 taccacgaac atgccgaggt tgctggcaat gacggcgcag acggcggcag ccatcaacat
    32881 attgatctgc aagccgttgc acttcacctg cgcgtggcga tgccagtcaa tgttatagag
    32941 gctgacaaac agaccgcaaa ggccgagggt aatcagccaa atcgcgttaa gcggggagat
    33001 ttgcacatcg tagcttacca gcgacagcgc accgctcacg ccaaccgcgc cagtcagcac
    33061 agtgaagccc gcggctgccg tatacaggct accaaccgcg ccgccaattc cggctatcca
    33121 gccacttagc gtcttttgaa aagaaaagag aaatgccaga acagcggcgg cgacaaacca
    33181 cgccacgccg ctattgatca gggaaattgc gctcatttag cctctccact ttgagcctgc
    33241 tgaaacaagg tgagatcgcc aaagtccgtg ttaaaggtca gctcacgctt acgtttgctg
    33301 acgcgggcga tatcggtgtt atccaccaga tgcagggctt tagtcgggca catccgcacg
    33361 caggccggac cttgttcatc aaagctacaa aggtcacatt tgacggcgat cgcgcgaata
    33421 cctggcaccc agtcaagcaa tgtgctgaca cgcgccggag caggcggtgc cggtggcgct
    33481 ttcggggtat tggcgtttgc cggaatatcc agcggacggc tgccggaaaa ttcaattgcg
    33541 ccaaacgggc aggcgatgcc gcacagcttg cagcttacgc acaggctttc attcaactgc
    33601 acggccccat caacgcgggt gatggcgtta accgggcaga ccaccgcgca gggtgcatct
    33661 tcacagtggt gacagagctg cggcgcagat tctttttcat tcagcatcac tctcaggcgc
    33721 ggcattgatt gcaggccgtg ctggcgatgc gtctctgaac aggcggcctc acaagtgtgg
    33781 cagccgatac agagcgtgga gtcagcaatt acaaaacgat tcaccaggca ttcctcaggt
    33841 gattgtcatt tttgacgaaa acatgccgat gaaatgtcat tttcgacact catcgacacg
    33901 cccatcccca aacaggcgta acgcctgcaa aacgggcaaa gcctcagctc atgctgccgg
    33961 gctttgtccc tttaccagtt ggcttaaatt caccggcaca ttgttttcaa tagcggtgta
    34021 taagctgtcg taaacgccag cgattttttc gagatagtgt tccagaacct gttcgcgatc
    34081 gcggatgttg accttgccat caatcatgcc gtcaatgctc agttgcgcct gcgcaatccg
    34141 ctgtttgtct tcgccatctt ttgtttcgac gtaatactcg atggtgtggc tgttaaagcc
    34201 atccgccagg gtgacgtaaa tgcgaaaatg ttcaaaaagg acgaaacaga gttctttgtc
    34261 cggcgcgcag ctcatggcgt gatgcaggcg cgctttcgat aacgtgatcc cctgaaccag
    34321 cgaattgcag tagatgtgcc actggtcctg taggcgacga tgccgctgtg cgatgtaatc
    34381 ggctttctcg cttatttccc aaatagtcat tgtcaggtta cccgtttaac agagatgcca
    34441 gctttaagca ttttctgtgc c
//
LOCUS       DAZGJW010000038        33923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZGJW010000038 DAZGJW010000000
VERSION     DAZGJW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.038"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(122..874)
                     /gene="tcyN"
                     /locus_tag="ACHKIK_001858"
     CDS             complement(122..874)
                     /gene="tcyN"
                     /locus_tag="ACHKIK_001858"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO9083041.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(871..1539)
                     /gene="tcyL"
                     /locus_tag="ACHKIK_001859"
     CDS             complement(871..1539)
                     /gene="tcyL"
                     /locus_tag="ACHKIK_001859"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO9083042.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1554..2540)
                     /gene="dcyD"
                     /locus_tag="ACHKIK_001860"
     CDS             complement(1554..2540)
                     /gene="dcyD"
                     /locus_tag="ACHKIK_001860"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO9083043.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2645..3445)
                     /gene="tcyJ"
                     /locus_tag="ACHKIK_001861"
     CDS             complement(2645..3445)
                     /gene="tcyJ"
                     /locus_tag="ACHKIK_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9083044.1"
                     /translation="MKLAHLGRQALMSVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3533..4084)
                     /gene="fliZ"
                     /locus_tag="ACHKIK_001862"
     CDS             complement(3533..4084)
                     /gene="fliZ"
                     /locus_tag="ACHKIK_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO9083045.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4130..4849)
                     /gene="fliA"
                     /locus_tag="ACHKIK_001863"
     CDS             complement(4130..4849)
                     /gene="fliA"
                     /locus_tag="ACHKIK_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO9083046.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5014..6477)
                     /locus_tag="ACHKIK_001864"
     CDS             complement(5014..6477)
                     /locus_tag="ACHKIK_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFO9083047.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRVRELTVQATNG
                     TNSDSDLSSIQAEITQRLEEIDRVSEQTQFNGVKVLAENNEMKIQVGANDGETITINL
                     AKIDAKTLGLDGFNIDGAQKATGSDLISKFKATGTDNYDVGGDAYTVNVDSGAVKDTT
                     GNDIFVSAADGSLTTKSDTNIAGTGIDATALAAAAKNKAQNDKFTFNGVEFTTTTAAD
                     GNGNGVYSAEIDGKSVTFTVTDADKKASLITSETVYKNSAGLYTTTKVDNKAATLSDL
                     DLNAAKKTGSTLVVNGATYDVSADGKTITETASGNNKVMYLSKSEGGSPILVNEDAAK
                     SLQSTTNPLETIDKALAKVDNLRSDLGAVQNRFDSAITNLGNTVNNLSSARSRIEDAD
                     YATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            6732..8144
                     /gene="fliD"
                     /locus_tag="ACHKIK_001865"
     CDS             6732..8144
                     /gene="fliD"
                     /locus_tag="ACHKIK_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO9083048.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGNYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDSAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            8159..8569
                     /gene="fliS"
                     /locus_tag="ACHKIK_001866"
     CDS             8159..8569
                     /gene="fliS"
                     /locus_tag="ACHKIK_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO9083049.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8569..8934
                     /gene="fliT"
                     /locus_tag="ACHKIK_001867"
     CDS             8569..8934
                     /gene="fliT"
                     /locus_tag="ACHKIK_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO9083050.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9012..10499
                     /gene="amyA"
                     /locus_tag="ACHKIK_001868"
     CDS             9012..10499
                     /gene="amyA"
                     /locus_tag="ACHKIK_001868"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO9083051.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10533..10946)
                     /gene="yedD"
                     /locus_tag="ACHKIK_001869"
     CDS             complement(10533..10946)
                     /gene="yedD"
                     /locus_tag="ACHKIK_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO9083052.1"
                     /translation="MKKLAIASALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11133..12338
                     /gene="yedE"
                     /locus_tag="ACHKIK_001870"
     CDS             11133..12338
                     /gene="yedE"
                     /locus_tag="ACHKIK_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO9083053.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12335..12568
                     /gene="yedF"
                     /locus_tag="ACHKIK_001871"
     CDS             12335..12568
                     /gene="yedF"
                     /locus_tag="ACHKIK_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO9083054.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12675..13343
                     /gene="yedK"
                     /locus_tag="ACHKIK_001872"
     CDS             12675..13343
                     /gene="yedK"
                     /locus_tag="ACHKIK_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO9083055.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNIKNQGAEL
                     IQPV"
     gene            complement(13738..14052)
                     /gene="fliE"
                     /locus_tag="ACHKIK_001873"
     CDS             complement(13738..14052)
                     /gene="fliE"
                     /locus_tag="ACHKIK_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO9083056.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHATLDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            14267..15925
                     /gene="fliF"
                     /locus_tag="ACHKIK_001874"
     CDS             14267..15925
                     /gene="fliF"
                     /locus_tag="ACHKIK_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO9083057.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            15918..16913
                     /gene="fliG"
                     /locus_tag="ACHKIK_001875"
     CDS             15918..16913
                     /gene="fliG"
                     /locus_tag="ACHKIK_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO9083058.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            16906..17592
                     /gene="fliH"
                     /locus_tag="ACHKIK_001876"
     CDS             16906..17592
                     /gene="fliH"
                     /locus_tag="ACHKIK_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO9083059.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQMPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            17592..18965
                     /gene="fliI"
                     /locus_tag="ACHKIK_001877"
     CDS             17592..18965
                     /gene="fliI"
                     /locus_tag="ACHKIK_001877"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO9083060.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            18984..19427
                     /gene="fliJ"
                     /locus_tag="ACHKIK_001878"
     CDS             18984..19427
                     /gene="fliJ"
                     /locus_tag="ACHKIK_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO9083061.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            19424..20551
                     /gene="fliK"
                     /locus_tag="ACHKIK_001879"
     CDS             19424..20551
                     /gene="fliK"
                     /locus_tag="ACHKIK_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO9083062.1"
                     /translation="MIRLAPLITADVDNATLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAVPVLSAPLGSHEWQQSLSQHISLFTRQRQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLLVPVSIQGRVTGNSGVDIFA"
     gene            20656..21120
                     /gene="fliL"
                     /locus_tag="ACHKIK_001880"
     CDS             20656..21120
                     /gene="fliL"
                     /locus_tag="ACHKIK_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO9083063.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            21125..22129
                     /gene="fliM"
                     /locus_tag="ACHKIK_001881"
     CDS             21125..22129
                     /gene="fliM"
                     /locus_tag="ACHKIK_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO9083064.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            22126..22539
                     /gene="fliN"
                     /locus_tag="ACHKIK_001882"
     CDS             22126..22539
                     /gene="fliN"
                     /locus_tag="ACHKIK_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO9083065.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            22542..22907
                     /gene="fliO"
                     /locus_tag="ACHKIK_001883"
     CDS             22542..22907
                     /gene="fliO"
                     /locus_tag="ACHKIK_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO9083066.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            22907..23644
                     /gene="fliP"
                     /locus_tag="ACHKIK_001884"
     CDS             22907..23644
                     /gene="fliP"
                     /locus_tag="ACHKIK_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO9083067.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            23654..23923
                     /gene="fliQ"
                     /locus_tag="ACHKIK_001885"
     CDS             23654..23923
                     /gene="fliQ"
                     /locus_tag="ACHKIK_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO9083068.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            23932..24717
                     /gene="fliR"
                     /locus_tag="ACHKIK_001886"
     CDS             23932..24717
                     /gene="fliR"
                     /locus_tag="ACHKIK_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO9083069.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLADTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLILTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            25007..25630
                     /gene="rcsA"
                     /locus_tag="ACHKIK_001887"
     CDS             25007..25630
                     /gene="rcsA"
                     /locus_tag="ACHKIK_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO9083070.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(25674..25862)
                     /gene="dsrB"
                     /locus_tag="ACHKIK_001888"
     CDS             complement(25674..25862)
                     /gene="dsrB"
                     /locus_tag="ACHKIK_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO9083071.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            26025..26252
                     /gene="yodD"
                     /locus_tag="ACHKIK_001889"
     CDS             26025..26252
                     /gene="yodD"
                     /locus_tag="ACHKIK_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO9083072.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            26550..27365
                     /gene="yedP"
                     /locus_tag="ACHKIK_001890"
     CDS             26550..27365
                     /gene="yedP"
                     /locus_tag="ACHKIK_001890"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO9083073.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQSSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAH"
     gene            complement(27362..29056)
                     /gene="dgcQ"
                     /locus_tag="ACHKIK_001891"
     CDS             complement(27362..29056)
                     /gene="dgcQ"
                     /locus_tag="ACHKIK_001891"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO9083074.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFVGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLHWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(29294..29476)
                     /gene="yodC"
                     /locus_tag="ACHKIK_001892"
     CDS             complement(29294..29476)
                     /gene="yodC"
                     /locus_tag="ACHKIK_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO9083075.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(29555..30472)
                     /gene="yedI"
                     /locus_tag="ACHKIK_001893"
     CDS             complement(29555..30472)
                     /gene="yedI"
                     /locus_tag="ACHKIK_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO9083076.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVAGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            30645..31565
                     /gene="yedA"
                     /locus_tag="ACHKIK_001894"
     CDS             30645..31565
                     /gene="yedA"
                     /locus_tag="ACHKIK_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO9083077.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIPDASSE"
     gene            complement(31554..32024)
                     /gene="vsr"
                     /locus_tag="ACHKIK_001895"
     CDS             complement(31554..32024)
                     /gene="vsr"
                     /locus_tag="ACHKIK_001895"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO9083078.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVLDEFRCVIFAHGCFWHHHHCYLFKVPATRTDFWLEKIGKNVERDRRDIS
                     HLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(32005..33423)
                     /gene="dcm"
                     /locus_tag="ACHKIK_001896"
     CDS             complement(32005..33423)
                     /gene="dcm"
                     /locus_tag="ACHKIK_001896"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO9083079.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISKCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(33490..>33923)
                     /locus_tag="ACHKIK_001897"
     CDS             complement(33490..>33923)
                     /locus_tag="ACHKIK_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFO9083080.1"
                     /translation="FVQFPAEKIEAVCHAIAAHSFSAQIAPLTTEAKIVQDADRLEAL
                     GAIGLARVFAVSGALGLALFDGEDPFAQHRPLDDKRYALDHFQTKLLKLPQTMQTARG
                     KQLAQHNAQFLVEFMAKLGAELAGENEGIDHKVIDAFSPAG"
BASE COUNT         8281 a         8804 c         8604 g         8234 t
ORIGIN      
        1 ttgcaagcca tcacattctc ttatgttatt aatgagttat gctgatttgt taagcagttt
       61 tatcacgctt gaaatagcgt cccgccccga caggtgaatc gtcggggctg attttttctt
      121 attattgcag cagaaacttc tcgaggaact ggcgggtgcg aggctgctgg ggatcggcaa
      181 ataacgcttt tgctgccccc tgctcgacta tccgcccctg gtccataaaa atcgcccggt
      241 ccgcaacatc ccgggcaaag ctcatttcgt gcgtcacaat caccatcgtg cgcttttcct
      301 gcgccagctg acggatggtg ttcaggactt cgcccaccag ctctggatcc agcgccgacg
      361 ttggctcgtc aaacaaaatc acctcaggac gcattgccag cgcacgtgca atcgcaacac
      421 gttgctgttg accgccagac aaacgacgtg gatagctggt ttctttaccg gccagcccaa
      481 ctttcgccaa cagctcgcga gcgcgcgccg tggcctcttc tttcggttcg cctttgacga
      541 tcaccggccc ttcaataatg ttttccagca ccgtacgatg cggaaacaaa ttaaagttct
      601 ggaagacaaa cccgacgtgc tgacgcaact ggcgaatcag cgatttttgc tgacttaatg
      661 aacgtgcagt atcaatagtg atatcgccaa cggtgatcgt tcccgcttcc ggttgttcca
      721 gcagatttat gctgcgtagc aacgtggttt tgccggaacc actcggaccg ataattgcca
      781 ccacttcgcc aggctttacc tcgaggtcga taccgtgcag caccgtctga ccgtgaaatt
      841 ttttcaccaa gttcttaact tcaatggcac tcattttggt tctctctcct ggcgattaag
      901 ttgattctca aaatggttct gcaacgtcga taatactgtc gccatgatcc agtagatgag
      961 cgaagccgcc aggtacatgg tgaacacctc cagcgtgcgc gaggtaatca actgcgcctg
     1021 acggaacagc tccggcacct ggattgtcgc ggccagcgac gtatccttta ccaggctgat
     1081 aaagctgttc gacagcggcg gcaacgccac ccgcgccgcc tgtggcaaaa tcgcgcgacg
     1141 catagtctgc catggcgtca taccaatact ggcagctgct tcccactgac ctttatcaat
     1201 agaagaaatg gcggcgcgca gtgtttcggc ggcataggcg gcagtgttca gcgacagacc
     1261 aatcattgct gacggaatgg gatctaattc gataccaaac tgcggcaggc cgtaatagat
     1321 cataaacagt tgtgcgatga gcggtgtacc acgaaaaata gagatataaa aacgcgccag
     1381 ccagcgcacg ggccagattg gcgacaggcg catcagcgcg agaataaacc cgagcagtaa
     1441 gccaaaaaac atgccgccga tactgagttg cagcgtatac ccagcccctt tcagcaggaa
     1501 cggcaaggaa tcaataacca gttgtatact ttcttgcatg agcgttcccg gagctaaacg
     1561 tggggatgat aggcgaacag cgcaggcgca ccgccggtat gaataaacag aatcggccct
     1621 tcatctttga agcgtttctg actgataccg tcaatcagcc ccgccatcgc ttttccggta
     1681 tacacaggat caagcagaat gccttccagc cgcgccagca atttcactgc ttccatgcct
     1741 tcgtcgttcg gcacgccgta gccaggtgca aaatagtcat cccagagtat aatttccgct
     1801 gatgcggtca gctccagttc tttcgcaatc gcctgttgta ggttaaccac tttcggcaac
     1861 tgatcggcaa cggaacgcga cacggtcacg ccaatcagtt cgctttcagg catcaggtgt
     1921 tccagcccaa cagccagtcc ggcgtgagtt ccggcactgc ccgatgcgac taccaccgac
     1981 gaaatattaa ccgccccttc acactgttgc gcgatttcca gcgcactctc cacataacct
     2041 agcgcgccaa gagcattaga accgccaacc ggaatgacat acgggcgaaa gccttgtgct
     2101 tcgactcgcg tcgccagctc ttccagttgg gcattgggat cggtcagtgc ttcgcacatt
     2161 tcaatctggg tattgaacag atccagcaac aagcgattgc cgttggttaa atagttttct
     2221 gcggttgtgc caataggatt ttccagcagc gccacacagt gcagaccgag tttcgccgca
     2281 actgcggcag tctgccgcac atggttagac tggatcgccc cggcagtaat cagcgtatcg
     2341 gcaccttcgc gcagagcatc tgctgcgaga aattccagct tacgtaattt attgccgccc
     2401 attgccatgg gggtgacgtc atcccgtttg atgaaaattt cccgtccaag ataatcagaa
     2461 aagcgcggca gatattcgag cggtgttggc gcgccgataa actccaaccg tggaaaacgg
     2521 gttaaattat gcagtggcat aacagcctcc gatgtgtgtt gttgtgattt tcttattatg
     2581 cacgctgaaa acgcgtaaat aaaaaaggcg ctggtgaaag cgcccttttt tgtcattatg
     2641 ctgattattt ggtcacatca gcaccaaacc atttttcgga aagggcttgc agagtgccat
     2701 ctttttgcat ttccgcaatt gcatcgttca ctgctttcag caggtcttca tttcctttac
     2761 gcagcgccac gccagactcc tgacgggaga atgcttcacc ggttactgcc agcgtatcgt
     2821 tggttttctt caccagatcc agcgccgcca gacggtcaac gaggatcgca tcgatacggc
     2881 ctacgcgcag atcctgatat ttggtcgggt catcatcata ggtacgcaca tcgacgccct
     2941 gaacattctg ccgcagccac tcttcatagt tggtgcccag accgacaccc acttttttgc
     3001 ctttcagatc agcagccgtt ttaatggtgc cttcgttacc ttttttcacc agcgcctgaa
     3061 taccagaaat ggtgtacggg gtagagaaat cgtatttttt cttgcgctca tcagaaatgg
     3121 tgacctgatt aatcaccaca tcaatgcgtt tagagtccag cgacgccagc ataccgtccc
     3181 atttggtcgg ttttagtgac gcctcaacgc caagatgttt tgccagctgt tgggcaaatt
     3241 ccacttcaaa accggttaat ttgccgtcat ccccctgaaa actgaacggc ggataagtgc
     3301 cttccagccc taccagcagc gtgccgcgct ctttaacttt attaagcaga ccttcatctg
     3361 caaaactttt aacgctcatg cccgcaacca gcgctacggc catcacactc atcaatgcct
     3421 gacgtcccag atgtgctaat ttcatattta ccccgaatgt tgttatgtct gtttgcagtg
     3481 tagagccatt gtttgtaaac acaaaaacaa ctccgctaca tcttattctt atttaatata
     3541 tatcagaagc aggcaggctg gaggatttct gcacaagtcc ggtacacgtt tgacgctgat
     3601 agtgttgata cttccgtaat gcgatgcggt aattgttggt gctggtggtg ggcagccacg
     3661 gggcgaggat ttcatcaagg aaaccgtcct gcagcggatc gagggaaata ttttgctcgt
     3721 gtagatgatt ccccaaacgg cgcaaacgaa cgacatattc acgcaccgta cctgggctca
     3781 tttcagtttg ctcaaaaaga aattgcttaa agccgataat atcgaaaaaa tcactctgcg
     3841 ttttgcaatg taaatcaccg caaaatcggc acaatgccgc ccatgatttt tgttccgttt
     3901 gccagccatt ttcatcaagc agcgcgtcca ggcgggaaat ctcgatttta ttcactattt
     3961 tgccgtcgcg aacgagggta atgcgatcga gtaatttacg gcaatgcgcg caatgagtct
     4021 ggctgtgttt aaagtcttta agatagcggc ttaatggccg tcttttcagg tgctgcacca
     4081 tcattaagaa ctcctggtag tcaaagttaa agtgcggcat ttactgacgt tataacttac
     4141 ccagtttagt gcgtaaccgt ttaatagcct ggctgtgtaa ctgactgacc cgcgattccc
     4201 cgacctccag caccgcgcca atctctttga gattcagctc ttcctggtaa tagagggtta
     4261 ataccagttt ttcgcgctcc ggcaacgttt cgatggcttc catcacccgc tgacgcagat
     4321 tactgtccag cagttgttgt agcgggtttt ctcgctgatg atcatcagta accagttcga
     4381 tgctatcgcc gtgctcttcg cgccactcat cgtaggagaa gagctggctg ttattggtgt
     4441 cgagcaacat ttggcgataa tcggcaatat cgatccctaa acgttccgct acctcagttt
     4501 ccgtggcgtt gcggccaagt tcctgctcca gttgccctat tgcctgtgcc acttcacgcg
     4561 cgttgcgtcg cacgctgcgc ggcacccagt cacggctgcg aagttcatcc agcatagcgc
     4621 cacggatacg ctgcactgcg taagttgtaa atgccgttcc ttgtagggcg tcatagcgtt
     4681 cgacggcatt aagtaaccca atgccgcccg cctgtagcag atcgtcaagt tccacgctcg
     4741 cgggcagtcg aacctgcagg cgcaatgctt cgtgacgcac cagcgggaca taacgctgcc
     4801 atagcgagtg tttatccatt acaccttcag cggtatagag tgaattcacg ataaacagcc
     4861 ctgcgttata tgagttatcg gcatgattat ccgtttctgc agggttttta atcggacgat
     4921 tagtgggtga aatgaggggt tatttggggg ttaccggtaa attgcgggca gaaaaaaccc
     4981 cgccggtggc gggggttgag cgataagtgt aaattaacgc agcagagaca gtacgttctg
     5041 cgtggtctgg ttagcctgcg ccagaacaga ggtacccgct tgttgcagga tctgcgcacg
     5101 agacatgtta gacacttcgg tcgcgtagtc agcatcttcg atacggctac gggcagaaga
     5161 caggttgttt acggtgttgc caaggttggt gatagcagag tcgaaacggt tttgtactgc
     5221 accgaggtca gaacgcagat tgtcaacttt agccaatgct ttgtcgatag tttcgagcgg
     5281 gttggtggta gattgcaacg attttgctgc atcttcgttt accagaatcg ggctaccacc
     5341 ttctgatttg ctcagataca tgactttatt gttaccagaa gcagtctccg ttatcgtttt
     5401 accatctgca ctaacatcgt aagttgcacc gttaacaact aacgtgcttc ctgttttctt
     5461 agctgcattg agatcaagat cggaaagtgt ggcagccttg ttatcaactt tggttgtcgt
     5521 ataaaggcca gcgctatttt tgtaaactgt ctcactcgta atcaaagaag cttttttgtc
     5581 agcatctgtc acagtaaatg tcactgactt accatcaatt tctgcagaat atacaccatt
     5641 cccattgcca tccgctgcag ttgttgttgt gaattcaact ccattaaacg tgaatttatc
     5701 attctgtgct ttattcttag ccgctgctgc gagtgctgta gcatcaatcc ctgtaccagc
     5761 tatgtttgtg tcagatttag ttgtcagtga accatctgct gcactaacaa aaatatcatt
     5821 ccctgtagta tctttaacag ctccgctatc tacgttaaca gtataagcat caccgccaac
     5881 atcatagtta tcagtacctg tcgctttaaa tttagaaatc aggtcactgc cagttgcttt
     5941 ctgcgcgcca tcgatattaa aaccgtccag gccgagagtt ttcgcatcaa tttttgccag
     6001 attgatagtg atggtttcac catcattagc accaacctga attttcattt cattattttc
     6061 agcaaggact ttcacgccgt taaactgagt ttgctcagat acacggtcaa tttcttccag
     6121 acgttgagta atttcagcct ggatagaaga aagatcgctg tcagagttag taccgttagt
     6181 tgcctgaaca gtcagttcac gtacacgctg caggttgttg ttaatttcat tcagcgcacc
     6241 ttcagtggtc tgcgcaacag aaataccatc attcgcgtta cgggaagcct gagtcagacc
     6301 tttaatattt gctgtaaaac ggttagcaat cgcctgacct gctgcgtcat ctttagcact
     6361 gttaatacgc aggccagaag agagacgttc aatggcggag ctcagagaag actgagattt
     6421 gttcaggtta ttctgggtca acagcgacag gctgtttgta ttaatgactt gtgccatgat
     6481 cttttcctta tcaattacaa cttgatgtta ttgggctgtt gcccacggtt tctcaccgta
     6541 ccccttgtat cggcacctga atttcgaact ttagaaaatt ttagtttctc tcctgttttt
     6601 tcttaacggc tgaaatatcc gtaatataca actctactct gcgcatcatc ttcgtgaatc
     6661 aactattaaa ctttgcgcac aaattgccga taacccgctt atctactgtt tgcaatcaaa
     6721 aggaattagg tatggcttca tttacatctc ttggcgttgg ttcgaatctg ccgctggata
     6781 cattgctaaa caacctgacc atcgctgaaa aaaaacgctt aaacccaatt actcagcaac
     6841 agagtgacaa taccgcacgt ctgacagcct atggcacatt aaaaagtgca ttagaaaaat
     6901 tccagactgc aaatactgcg ttaaataaag ccgatctgtt caaaagcact accgttacca
     6961 gtagcaatga agaccttaaa gtttccaccg aagcaggtgc tgcccccggt atctatacca
     7021 tcagcgtaac ccaactggcg caggcgcaat ctttacgaac tgattccccg acaatcattg
     7081 catcaacaaa agatgcactg ggtgatgagt catcagatac acgcacaata aaaattaccc
     7141 aagacggtcg taaagagccg ctggaaatca agctcaataa agatcagacg tcattggatg
     7201 agatcagcaa agccattaac gatgccgata gtggaatttc cgctagtatc gttaaagtca
     7261 aagatggtaa ctatcaattg gtattgaccg ccagtgaagg tctggccaat aaaatgacca
     7321 tatctgttga gggtgacagt aaattaaatg atctgctggc ttatgacagc aagaccaaca
     7381 ccgggaatat gaaggaactg gttaacgcgc aaaatgcaca gctaaacgtt aacggtatcg
     7441 atattgaacg ttcaagtaac aaaattaccg atgctcctca gggagtgact ctcgatttga
     7501 ccaaaaaagt cactgacgtc cgggtcacgg tgacaaaaag caacgataaa gcaaccgaag
     7561 caattaaagg ctgggtggat tcatataact ccctcattga cacctttaac accctgacaa
     7621 aatataaaga agtagaccca ggtgctgaag cacaagataa aaataatggt gcacttctcg
     7681 gagacagcgt tgtacgtaca attcaaagcg gcatccgcgc acaatttgca aacggtgcca
     7741 gcgacagtgc cttcaaaaca ttaaacgaga tcgggattaa gcaggacggc acgacgggta
     7801 agctgaaaat tgatgatgat aaactcaaaa aagtcctgaa tgaaaataca gcatccgtac
     7861 gtgaattgct ggtaggcgat ggtaaagaaa caggaattac caccaaaatt gccaccgagg
     7921 taaaaggtta ccttgccgat gatggcatta ttgatagcgc acaggacagt attaacgcca
     7981 cactgaaaaa actgaccaaa cagtatctat ccgtcagtgc cagcattgat gacaccgtgg
     8041 cgcgttacac tgcacagttt acgcaattag ataccatgat gagcaaactg aataacacca
     8101 gtacctatct cagccaacaa tttactgcaa tgagtaattc ctgatcacgg aggtcagcat
     8161 gtataccgcg agcggctcaa aagcctatgc acaaattggc gtcgaaagcg ccgtaatgag
     8221 cgccagccag cagcagctgg tcaccatgct atttgatgga gtgctgagcg cactggttag
     8281 agcacgcctg tttatgcagg acaacaatca gcaaggcaaa ggcgtctctt tgtcaaaagc
     8341 gatcaacatc attgagaacg gactgcgggt gagtcttgat gaagagagca aagacgaatt
     8401 aacccaaaac ttgattgctc tttatagcta tatggtcagg cgcttgctgc aagccaattt
     8461 acgcaacgat gtctccgcag tcgaagaagt ggaagcatta atgcgcaata ttgccgatgc
     8521 ctggaaagag tcgttactct ccccttcttt gattcaggac ccagtctgat gaaccacgca
     8581 ccgcatttgt atttcgcctg gcaacaactc gtcgaaaaaa gccagctcat gttacgcctg
     8641 gcaacagaag aacaatggga cgaactcatc gccagcgaga tggcgtatgt gaatgcggtg
     8701 caggagattg cacatctgac tgaagagatt gacccgtcca ccacgatgca ggagcagctc
     8761 cgcccgatgc tgcgcctgat tctcgacaac gaaagcaagg taaagcagtt attacagatt
     8821 cggatggatg aactggcgaa actggtcggt cagtcatcgg tgcaaaaatc ggtgttaagt
     8881 gcctatggtg atcagggcgg ctttgtgctg gctccgcagg ataacctctt ttgaatctga
     8941 acgagtcgat ggctcgcgaa taatccgatt acggctacgc ttctaatgtt ccccttgaat
     9001 ggagtcgaag aatgcgtaat cccacgctgt tacaatgttt tcactggtat tacccggaag
     9061 gcggtaagct ctggcctgaa ctggccgagc gcgccgacgg ttttaatgat attggtatca
     9121 atatggtctg gttgccgccc gcctataaag gcgcatcggg cgggtattcg gtcggctacg
     9181 actcctatga tttatttgat ttaggcgagt ttgatcagaa aggcagcatc cctactaaat
     9241 atggcgataa agcacaactg ctggccgcca ttgacgccct gaaacgtaat gacattgcgg
     9301 tgctgttgga cgtggtagtc aaccacaaaa tgggcgcgga tgaaaaagaa gctattcgcg
     9361 tgcagcgtgt aaatgctgat gaccgtacgc aaattgacga agaaatcatt gagtgtgaag
     9421 gctggacgcg ttacaccttc cccgcccgtg ccgggcaata ctcgcagttt atctgggatt
     9481 tcaaatgttt tagcggtatc gaccatatcg aaaaccctga tgaagatggc atatttaaaa
     9541 ttgttaacga ctacaccggc gaaggctgga acgatcaggt tgatgatgaa ttaggtaatt
     9601 tcgattatct gatgggcgag aatatcgatt ttcgcaatca tgccgtgacg gaagagatta
     9661 aatactgggc gcgctgggtg atggaacaaa cgcaatgcga cggttttcgt cttgatgcgg
     9721 tcaaacatat tccggcgtgg ttttataaag agtggatcga acacgtacag gaagttgcgc
     9781 caaagccgct gtttattgtg gcagagtact ggtcgcatga agtcgataaa ctgcaaacgt
     9841 atattgatca ggtggaaggc aaaaccatgc tgttcgatgc gccgctgcag atgaaattcc
     9901 atgaagcatc gcgcatgggg cgcgactacg acatgacgca gattttcacg ggtacattag
     9961 tggaagccga tcctttccac gccgtgacgc tcgttgccaa tcacgacacc caaccattgc
    10021 aagccctcga agcaccggtc gaaccgtggt ttaaaccgct ggcgtatgct ctaattttgt
    10081 tgcgggaaaa tggcgttcct tcggtgttct atccggacct ctacggtgcg cattacgaag
    10141 atgtcggtgg tgacgggcaa acctatccga tagatatgcc aataatcgaa cagcttgatg
    10201 agttaattct cgcccgtcag cgtttcgccc acggtgtaca gacgttattt ttcgaccatc
    10261 cgaactgcat tgcctttagc cgcagtggca ctgacgaata tcccggctgc gtggtggtca
    10321 tgtcgaacgg ggatgatggc gaaaaaacca ttcatctggg agagaattac ggcaataaaa
    10381 cctggcgtga ttttctgggg aatcggcaag agagcgtagt gaccgacgaa aacggcgaag
    10441 cgaccttctt ttgcaacggc ggcagcgtca gcgtgtgggt tatcgaagag gtgatttaaa
    10501 ttcatccccg gcggcaagcc ggggagattt cattacggca gtggcgtcgg taacggcgct
    10561 ttatccagcg cggcggcaca ctctgccgtc gggcgatcca cacgctccat cgtcatacca
    10621 tcgtactcaa tggtgttacc gtcacgctca acctcgtaca gctcacgttt tacggtaacg
    10681 ttagtgagat catcagacat caatgtcaac ttgcccggca cggcaatcac ccgctgccac
    10741 tgacggcaat ccagggtatc gccctcttta gtgacaatca ggctgcctat cgcttccgga
    10801 ctgaccagcg cacgttgcgg ccctttggtc tgccagtagc ccgctagcca gtccggcgcg
    10861 ggggttttca cgacattgtt atagttttcg acttcggcac agcccgccag caacatgagc
    10921 gcgcttgcaa ttgcgagttt tttcatcatt cgtcctgcat gagaagaaaa gaggattgtg
    10981 gcattaaagc cctgatgtcg ccagcctttc caggttcaga gagaaatttg atctaactat
    11041 tatttttgtg aattattacc ccgaaaataa atttgcatga tgtatgattc gcgattcttg
    11101 tttcaaacct tctgagttca gaggctacat tcatgtcatg gcagcaattc aaacacgcct
    11161 ggttgattaa attctgggca cccatccctg cggtcatcgc ggcgggtatt ctctccactt
    11221 actattttgg cattactggc accttttggg ctgtcacggg tgaatttacc cgttggggcg
    11281 gccagctcct gcagctgttt ggcgtccatg ctgaagagtg gggttatttt aaaattatcc
    11341 atctggaagg atcgccatta acccgcatcg acgggatgat gatcctcggc atgtttggcg
    11401 gctgctttgc cgcagcgctg tgggccaaca atgtcaaact gcgcatgcca cgcagccgta
    11461 tccgcattat gcaggccatt attggcggta ttatcgctgg ttttggcgcg cgtctggcaa
    11521 tgggctgtaa cctggcggcg ttctttaccg gtattcctca gttctcgctg catgcctggt
    11581 tctttgccat cgccactgcc attggttcat ggtttggcgc acgctttacc ctgctgccca
    11641 tcttccgtat tcccgtgaaa atgcagaaag tttctgccgc ctcaccgctg acgcaaaaac
    11701 cggatcaggc gcggcgtcgt tttcgtctcg ggatgctggt ctttttcggt atgctgggct
    11761 gggcgctgct cacggcgatg aaccaaccaa aactggggct ggcaatgctg tttggcgtcg
    11821 gctttggttt actgattgaa cgtgcgcaaa tctgctttac ttcggcgttc cgcgatatgt
    11881 ggatcaccgg acgtacccat atggcgaaag caatcattat cggtatggcg gtgagtgcca
    11941 tcgggatctt cagttacgta cagttaggcg ttgaacccaa aatcatgtgg gcgggaccaa
    12001 acgcggtaat tggtggttta ctgtttggtt ttggcatcgt gctggcaggc ggctgcgaaa
    12061 ccggctggat gtaccgcgcg gtagaaggcc aggtgcacta ctggtgggtc ggtctgggca
    12121 atgtgatcgg ctcgacgatt ctggcgtatt actgggatga tttcgctccg gcgctggcca
    12181 ccgactggga caaaatcaac ctgctgaaaa cctttggccc gatgggtggc ctgctggtga
    12241 catatttgct gttgtttact gcgctgatgt tgattatcgg ctgggaaaaa cgcttcttcc
    12301 gccgtgcggc accgcagact gctaaggaga tcgcatgaaa aatatcgttc ctgattaccg
    12361 ccttgatatg gtgggtgaac cctgccctta tccggcagtc gcaactcttg aggcgatgcc
    12421 gcagttaaaa aaaggggaaa tcctggaagt ggtgagcgac tgtccacagt cgatcaataa
    12481 tattccactg gatgcgcgta atcacggcta tacggtgctg gatattcagc aagacgggcc
    12541 gaccattcgt tatttaattc agaagtaatc cctcattccc gctgtggccc acagcgggaa
    12601 catcgttgcc tctgacacat agattttacc cgccccctct cctattcttt tcagtatgat
    12661 ctcaggaggt ttctatgtgt ggacgctttg cccaatccca aacgcgtgaa gattaccttg
    12721 cgcttctcgc ggaagatatt gaacgcgata ttccctacga tcccgaaccc attggcagat
    12781 acaacgtcgc gccgggaacc aaagttcttc tgctcagtga acgtgatgaa caccttcatc
    12841 tggatccggt tttctgggga tatgctcccg gatggtggga taaaccgccg ctgattaacg
    12901 cccgcgtaga aactgcggcc accagtcgta tgtttaaacc gctctggcaa catggtcggg
    12961 caatctgttt tgccgatggc tggtttgagt ggaaaaaaga aggcgacaaa aaacagcctt
    13021 attttatcta tcgcgctgat ggacaacctg tttttatggc cgcgataggt agcacaccgt
    13081 ttgagcgcgg tgacgaagcc gaaggatttt taattgtcac tgcagcagca gaccagggtc
    13141 tggtagatat tcatgaccgc cgcccgctgg ttctgtcgcc agaagctgca caggaatgga
    13201 tgcggcaaga gattggcggt aaagaagcat cagaaattgc taccaacggc tgtgtgccag
    13261 caaaccagtt cacctggcac ccggtatcgc gcgcggtggg taatatcaaa aaccagggag
    13321 cggagttaat tcaaccggtt tgataagcca ggagactatt gatcaataat actgcgtcat
    13381 aagaaatctc tattagacaa tagtagacgc tccttccaca tgcgccacaa gaaacctcta
    13441 ttccagtgac acaattacgc ctgattattt ccctagcccg gaatatgaat aaaaacatca
    13501 acaaagatta aattattcaa acgttactgt tgcgtctgcg gtcgtagcaa catccgacgt
    13561 ggtgatgagt gttaaaagag acactctgat gttcagaggg attatcgcgt ttacggatcg
    13621 tggatggcag aaaactttca ggatcaggta tccattttaa accagaaatt gagtgagttt
    13681 gccccatcca tgccccaagc ggtgagattg gaagtaatta acaacaggtt acaaaaccta
    13741 cacctgcatg gacatcactt cctgatacgc cgccaccagc ttattacgca cctgaatccc
    13801 catttgcata gaaactgagg ctttttgcat atcggtcatc acatcgttta acgccacgcc
    13861 gggttcaccg agggtgaatt tttctgcctg cgtgcgggca gctgtttgtg tatcgctaat
    13921 gcgatcgagc gtggcgtgca gctgcccggc aaaactaatg gtcggttgcg gcagtgaatc
    13981 ctgcgcacgc gcactcatcg ccgtagcctg taactggctg ataacccctt caatcccctg
    14041 tatcgctgac attttcgtct cctggataat ttctggtagc aaagcctacc agtaagtcaa
    14101 taagataaag gcgctaaata acaacaaaaa aacgggttta ttggcggata gaaaaaaacg
    14161 aaagcacaaa taatggcagc gtcaattttt cgagtttgct gacccgggag tgagtcttgt
    14221 tccactttgc caataacgcc gtccataatc agccacgagg tgcgcgatga atgcgactgc
    14281 agcccagaca aaatctcttg agtggcttaa tcgcctgcgt gcgaatccga aaattccatt
    14341 gattgttgcc ggttccgcgg cagtggcggt catggtcgca ctgatcctgt gggcgaaagc
    14401 ccccgactac cgcacattat tcagcaatct ttccgatcag gatggtggcg caattgtcag
    14461 ccaactgacg caaatgaata ttccttaccg cttcagcgaa gccagcggcg ctattgaagt
    14521 tccggcagat aaagttcacg aactgcgtct gcgcctggca caacaaggtt tgcccaaagg
    14581 cggcgcggtc ggtttcgaac tactcgatca ggaaaagttt ggtatcagcc agttcagcga
    14641 acaggtgaat tatcagcggg cgctggaagg cgagctttct cgtaccatcg aaactatcgg
    14701 cccggtaaaa ggggcgcgcg tacatctggc aatgccgaaa ccgtctttat tcgtccgtga
    14761 acaaaaatcc ccttctgcat cggtgacggt aaatctgtta cccggccgcg cactcgatga
    14821 agggcaaatt agcgccattg tgcatctggt ttccagcgcc gttgctggtc tgccgccggg
    14881 aaacgtcacg ctggtggatc agggcggaca tctgttaacc cagtccaata ccagcgggcg
    14941 cgatcttaat gatgctcagt tgaaatatgc cagcgatgtc gaaggccgta ttcagcggcg
    15001 tattgaagca atcctgtcgc ctattgttgg taacggtaat attcacgccc aggtcacggc
    15061 gcagctggac ttcgccagta aagaacaaac ggaagaacag tatcgcccta acggtgatga
    15121 atctcatgcg gcgcttcgtt cacgccagct taatgagagc gagcaaagcg gttccggtta
    15181 tccgggcggc gtaccggggg cgttgtcgaa tcaaccggca cctgcgaata acgcgccaat
    15241 cagcacgcct ccggcaaatc aaaataaccg ccagcagcag gcgagcacca ccagcaatag
    15301 cgggccgcgt agcacacagc ggaatgaaac cagtaactac gaagtcgatc gcaccattcg
    15361 tcataccaaa atgaacgtgg gcgatgtgca acgtctgtca gtcgcggtag tggtgaatta
    15421 caaaaccttg ccagacggta aaccattgcc tctcagcaac gaacagatga agcaaattga
    15481 agatctgacc cgcgaggcga tgggcttttc tgaaaaacgc ggtgactcgc tcaatgtcgt
    15541 taactcgccg ttcaatagca gtgacgaaag cggcggagaa ctgccgttct ggcaacagca
    15601 agcgtttatc gatcagttgc tggctgccgg tcgctggttg ctggtactgc tggtggcgtg
    15661 gctgctgtgg cggaaagcgg tacgcccgca gttaacacgt cgcgccgagg cgatgaaagc
    15721 tgtacagcaa caggcgcagg cccgtgagga agtggaagat gcggtggaag tccgcctgag
    15781 caaagacgaa caactccaac aacgacgcgc taaccaacgt ctgggggcag aagtcatgag
    15841 ccagcgtatc cgtgaaatgt ctgataacga tccgcgcgtg gtggcgctgg tcattcgcca
    15901 gtggataaat aacgatcatg agtaacctga caggcaccga taaaagcgtc atcctgctga
    15961 tgaccattgg cgaagaccgg gcggcagagg tgttcaagca cctctcccag cgcgaagtgc
    16021 aaaccctgag cgctgcaatg gcgaacgtca cgcagatctc caacaagcag ctaaccgatg
    16081 tgctggcgga gtttgagcaa gaagctgaac agtttgccgc actgaatatc aacgccaacg
    16141 attatctgcg ttcggtattg gtcaaagctc tgggtgagga acgtgccgcc agcctgctgg
    16201 aagatattct cgaaactcgc gataccgcca gcggtattga aacgctcaac tttatggagc
    16261 cgcagagtgc cgccgatctg attcgcgatg agcatccgca aattatcgcc accattctgg
    16321 tgcatctgaa gcgcgcccag gccgccgata ttctggcgtt gttcgatgaa cgtctgcgcc
    16381 acgacgtgat gttgcgtatc gccacctttg gcggcgtgca gccagccgcg ctggcggaac
    16441 tgaccgaagt actgaatggc ttgctcgacg gtcagaatct caagcgcagc aaaatgggcg
    16501 gcgtgagaac ggcagccgaa attatcaacc tgatgaaaac tcagcaggaa gaagccgtta
    16561 ttaccgccgt gcgcgaattc gacggcgagc tggcgcaaaa aattatcgac gagatgttcc
    16621 tgttcgaaaa tctggtggat gtcgacgatc gcagcattca gcgtctgttg caggaagtgg
    16681 attccgaatc gctgttgatc gcactgaaag gggccgagca gccactgcgc gagaaattcc
    16741 tgcgcaatat gtcgcagcgt gccgccgata ttctgcgcga cgatctcgcc aaccgtggtc
    16801 cggtgcgtct gtcgcaggtg gaaaacgaac agaaagcgat tctgctgatt gtgcgccgcc
    16861 ttgccgaaac tggcgagatg gtaattggca gcggcgagga tacctatgtc tgataatctg
    16921 ccgtggaaaa cctggacgcc ggacgatctc gcgccaccac aggcagagtt tgtgcccatg
    16981 gtcgagccgg aagaaaccat cattgaagag gccgaaccca gccttgagca gcaactggcg
    17041 caactgcaaa tgcaggccca tgagcaaggt tatcaggcgg gtattgccga aggtcgccag
    17101 caaggtcata agcagggcta tcaggaagga ctggcccagg ggctggagca aggtctggca
    17161 gaggcgaagt ctcaacaagc gccaattcat gcccggatgc agcaactggt cagcgaattt
    17221 caaactaccc ttgatgcact tgatagtgtg attgcttcgc gcctgatgca gatggcgctg
    17281 gaggcggcac gtcaggtcat cggtcagatg ccaacagtgg ataactcggc actgatcaaa
    17341 cagatccaac agttgttgca gcaagaaccg ttattcagcg gcaaaccgca actacgcgtg
    17401 caccccgatg atctgcaacg tgtggatgat atgctcggcg caaccttaag tttgcatggc
    17461 tggcgcttgc ggggcgatcc cacactccat cctggcggct gtaaagtctc cgccgatgaa
    17521 ggcgatctcg acgccagtgt cgccactcgc tggcaagaac tctgccgtct ggcagcacca
    17581 ggagtggtgt aatgaccacg cgcctgactc gctggctaac cacgctggat aactttgaag
    17641 ccaaaatggc gcagttgcct gcggtacgtc gctacgggcg attaactcgc gctaccgggc
    17701 tggtgctgga agccaccgga ttacaattgc cgctcggcgc aacctgtgtc attgagcgcc
    17761 agaacggcag cgaaacgcac gaagtagaaa gcgaagtcgt tggctttaac ggtcaacggc
    17821 tgtttttaat gccgctggag gaagtcgaag gtgtcctgcc cggcgcgcgt gtttatgcca
    17881 aaaacatttc ggcagaaggg ctgcaaagcg gcaagcagtt gccgctcggt ccggcgttat
    17941 taggtcgtgt tctggacggc agcggtaaac cgctcgatgg cctcccctcc cccgatacga
    18001 cggaaaccgg tgcgctgatt accccaccat ttaacccgtt gcaacgtaca ccgattgaac
    18061 atgtgctgga caccggcgtg cgcccaatca atgccctgct taccgttggg cgtgggcagc
    18121 gtatggggct gtttgccggg tccggcgttg gtaaaagtgt gctgctgggg atgatggcac
    18181 gttacacccg cgccgatgtc attgtcgtgg gtttgattgg tgaacgcgga cgcgaagtaa
    18241 aagattttat tgagaatatc ctcggtgccg aagggcgtgc acgctccgtg gtgattgccg
    18301 ctccggcgga tgtctctccg cttctgcgaa tgcaaggtgc cgcctatgcc acgcgaattg
    18361 ccgaagattt tcgcgatcgt ggtcagcatg tgttgctgat tatggactcc ctcacccgct
    18421 acgcgatggc ccagcgtgaa attgcgctgg cgattggcga accacctgcc actaaaggtt
    18481 atccaccgtc ggtgtttgcc aaattaccgg cactggtcga gcgtgccgga aatggaatta
    18541 gcggcggcgg ctcgattacc gcgttttata ccgtgctcac cgaaggcgat gaccagcagg
    18601 accccattgc cgactctgcg cgggcaatcc tcgacggtca cattgtgctg tctcgccgac
    18661 tggcggaagc cgggcactat ccggctatcg atattgaagc gtcgatcagc cgcgcaatga
    18721 cggcgttgat cagtgagcaa cattacgcgc gagtgcgcac cttcaaacag ctgttgtcga
    18781 gttttcagcg taaccgcgat ctggttagcg tcggcgcgta tgccaaaggc agcgatccga
    18841 tgctcgacaa agccatcgcc ctgtggccgc agctggaggg ctatttgcaa caaggcattt
    18901 ttgaacgcgc ggactgggaa gcgtctctcc aggggctgga gcgtattttc ccgacagtgt
    18961 cataacccag gagataacgg cagatggcag aacatggcgc gctggcgacc ctgaaagatc
    19021 tggcagaaaa agaggtagag gatgccgcgc gcctgctggg tgaaatgcgt cgcgggtgtc
    19081 agcaggcgga agaacagctc aaaatgctga tcgattatca gaatgaatat cgcaataacc
    19141 tcaacagcga tatgagtgcc gggatgacca gcaaccgctg gatcaactat cagcagttta
    19201 tccagacgct ggaaaaagcc attactcagc atcgccagca acttaatcag tggacgcaga
    19261 aagttgacat tgccctgaac agctggcgag aaaagaaaca acgtttgcag gcctggcaga
    19321 cactgcagga acggcaatcc acggcggcac tgcttgcaga aaaccgcctc gatcagaaaa
    19381 agatggatga gttcgcccag cgcgccgcca tgaggaaacc tgaatgattc gcttagcgcc
    19441 cttaattacc gccgacgttg ataacgccac attgcctggc ggcaaagcca gcgatgctgc
    19501 acaagatttt ctcgcgttgt tgagcgaagc attagcaggc gagacaacta ccgacaaagc
    19561 ggccccccag ttgctggtgg caacagataa gcccacgaca aaaggcgagc cgctggtcag
    19621 cgagattctt gccgatgcac aacaagcgga tttactgatc cctgtggatg aaacaccgcc
    19681 tgtcatcaac gacgaacaat ccacatcaac accattaacc accgctcaaa cgatgacgtt
    19741 ggctgcggtg gctgacaaca atacggcaaa agacgaaaaa gcggatgatc tgaatgaaga
    19801 cgtcaccgca agcctgagcg ccctttttgc gatgttgccg ggttttgaca atacgcccaa
    19861 agtgactgat gtgccgtcaa ccgtgttacc ggcagagaaa ccaacgctct tcacaaaact
    19921 gacttctgcg caactcacaa cagcacagcc tgatgatgcc cccggcacgc cagctcagcc
    19981 attaacaccg ctggtagcag aagcccagag taaagcggaa gtcatcagca caccttcacc
    20041 ggtgaccgct gccgccagcc cgctaatcac tccacaccag acacagccac tgcccaccgt
    20101 cgccgtgcct gtgttgagtg caccgctggg ttctcacgaa tggcaacaat cattaagcca
    20161 gcatatttcg ctgttcaccc gccagaggca acaaagtgca gagttgcgtc tgcacccgca
    20221 ggatttaggt gaagtgcaaa tctccctcaa agtggatgat aaccaggcac aaatccagat
    20281 ggtttcaccg catcagcatg tacgcgccgc gctggaagca gcgcttccgg tactgcgtac
    20341 gcagctggcc gaaagtggca ttcagttagg gcaaagcaac atcagtggcg aaagctttag
    20401 tggtcagcag caggccgctt cccaacaaca gcaaagccaa cgcacagtaa accatgaacc
    20461 tctggcgggg gaagacgacg atacgcttct ggttcccgtc tctatacaag ggcgcgtaac
    20521 aggcaacagc ggcgttgata ttttcgccta acgtcagagg tagcaccgta atccgcgtct
    20581 tttccccgct ttgttgcgct caagacgcag gataattagc cgataagcag tagcgacaca
    20641 ggaagaccgc aacacatgac tgattacgcg ataagcaaga aaagcaagcg atcgctttgg
    20701 atcccgattc tggtattcat taccctcgcg gcctgtgcca gcgcaggtta cagctactgg
    20761 cattcgcatc aggttgccgc tgacgacaaa gcgcagcaac gcgtcgtacc ctccccggtc
    20821 ttctacgcgc tggatacctt cacggtcaat ctgggcgatg cggatcgcgt gctttatatc
    20881 ggcataaccc tgcgcctgaa agatgaagct acccgctcgc ggctgagtga gtatttgccg
    20941 gaagtccgta gccgtttgct gttactgttt tcgcgtcagg atgctgccgt actggcgaca
    21001 gaagaaggca agaaaaacct gattgccgag attaaaacca cgctttccac cccgcttgtt
    21061 gccgggcaac cgaaacagga tgtcaccgac gtgctgtata ccgcttttat tctgcgataa
    21121 cgacatgggc gatagtattc tttctcaagc tgaaattgat gcgctgttga atggtgacag
    21181 cgaagtcaaa gacgaaccga cagccagtgt tagcggcgaa agtgacattc gtccgtacga
    21241 tccgaatacc caacgacggg ttgtgcgcga acgtttgcag gcgctggaaa tcattaatga
    21301 gcgctttgcc cgccattttc gtatggggct gttcaacctg ctgcgtcgta gcccggatat
    21361 aaccgtcggg gccatccgca ttcagccgta ccatgaattt gcccgcaacc tgccggtgcc
    21421 gaccaacctg aaccttatcc atctgaaacc gctgcgcggc acagggctgg tggtgttttc
    21481 accgagtctg gtgtttatcg ccgtggataa cctgtttggc ggcgatggac gcttcccgac
    21541 caaagtggaa ggtcgcgagt ttacccatac cgaacagcgc gtcatcaacc gcatgttaaa
    21601 gctggcgctt gaaggctata gcgacgcctg gaaggcgatt aatccgctgg aagttgagta
    21661 cgtgcgttcg gaaatgcagg tgaaatttac caatatcacc acctcgccga acgacattgt
    21721 ggttaacacg ccgttccatg tggagattgg caacctgacc ggcgaattta atatctgcct
    21781 gcctttcagc atgatcgagc cgctacggga attgttggtt aacccgccgc tggaaaactc
    21841 gcgtaatgaa gatcagaact ggcgcgataa cctggtgcgc caggtgcagc attcacagct
    21901 ggagctggtc gccaactttg ccgatatctc gctacgcctg tcgcagattt taaaactgaa
    21961 acccggtgac gtcctgccga tagaaaaacc cgaccgcatc atcgcccatg ttgacggcgt
    22021 cccggtgctg accagtcagt acggcactct caacggtcag tatgcgttac ggatagaaca
    22081 tttgattaac ccgattttaa attctctgaa cgaggaacag cccaaatgag tgacatgaat
    22141 aatccggccg atgacaacaa cggcgcaatg gacgatctgt gggctgaagc tttgagcgaa
    22201 caaaaatcaa ccagcagcaa aagcgctgcc gacgcggtgt tccagcaatt tggcggtggt
    22261 gatgtcagcg gaacgttgca ggatatcgac ctgattatgg atattccggt caagctgacc
    22321 gtcgagctgg gccgtacgcg gatgaccatc aaagagctgt tgcgtctgac gcaagggtcc
    22381 gtcgtggcgc tggacggtct ggcgggcgaa ccactggata ttctgatcaa cggttattta
    22441 attgcccagg gcgaagtggt ggtcgttgcc gataaatatg gcgtgcggat caccgatatc
    22501 attactccgt ctgagcgaat gcgccgcctg agccgttagt gatgaataac cacgctactg
    22561 tgcaatcttc cgcgccggtt tctgctgcgc cactgctgca ggtgagcggc gcactcatcg
    22621 ccattattgc cctgatcctc gctgctgcct ggctggtaaa acggttggga tttgtcccta
    22681 aacgcactgg cgttaacggt ctgaaaatta gcgccagtgc ttcgctgggc gcgcgtgaac
    22741 gggttgtggt ggtcgatgtg gaagatgcac ggctggtgct cggcgttacc gcagggcaaa
    22801 tcaatctgct gcataaactt cccccttcag caccaacgga agagataccg cagcccgatt
    22861 ttcagtcggt tatgaaaaac ttgcttaagc gtagcgggag atcctgatgc gtcgtttgtt
    22921 gtctgtcgca cctgtccttc tctggctggt tacgcccctc gccttcgcgc aactgccggg
    22981 tatcaccagc cagccgctgc ctggcggtgg acaaagctgg tcgctcccgg tgcaaacgct
    23041 ggtgttcatc acctcgttga cgtttattcc ggcaatttta ctgatgatga ccagtttcac
    23101 ccgcatcatc attgtttttg gtttattgcg taacgcgttg ggaacaccct ccgcgccacc
    23161 taaccaggta ttgctgggac tggcgctgtt tttgaccttt tttattatgt cgccggtgat
    23221 cgataaaatt tatgtcgatg cgtaccagcc attcagcgaa gagaaaatct caatgcagga
    23281 ggcgctggaa aaaggggctc agccgctgcg tgagtttatg ctgcgtcaga cccgcgaggc
    23341 ggatttaggg ttgtttgcca gactggcgaa tactggcccg ttgcagggac ctgaagctgt
    23401 gccgatgcgc attttgctcc cggcctacgt gaccagcgag ttgaaaaccg catttcagat
    23461 aggcttcacg attttcattc cttttttgat tatcgacctg gtgattgcca gcgtgttgat
    23521 ggcattgggg atgatgatgg ttcccccagc cacaattgct ctgcccttta aactgatgct
    23581 gtttgtactg gtggatggct ggcaattgct ggtcggttcg ctggcgcaga gcttttacag
    23641 ctagagaggc aaaatgacac ctgaatcggt catgatgatg gggactgaag cgatgaaagt
    23701 cgcgctggca ctggctgccc cactgttgct ggtggcgctg gtcacgggtc ttatcatcag
    23761 tattttgcag gccgccaccc agattaacga aatgacgctg tcgtttattc cgaaaatcat
    23821 cgccgtattt atcgccatta ttattgccgg accgtggatg ctcaatctgt tgctggatta
    23881 cgtccgcacc ttgttcacta acctgccgta tatcatcggg taggccgtac aatgatgcag
    23941 gtaacaagcg atcaatggct ttcctggtta agcctgtact tctggccgtt actgcgcgtg
    24001 ctggcgctca tttctaccgc accaattctg agcgaacgca gcgtgccgaa acgggtaaaa
    24061 ctgggtctgg caatgatgat cacgttcgct attgccccat cattacctgc caacgatgtt
    24121 cctgtttttt cgttctttgc tctgtggctg gccgtgcagc agatcctgat cggcattgcg
    24181 cttggtttta ccatgcaatt tgcctttgcc gctgtgcgaa ccgctggtga aattatcggt
    24241 ctgcaaatgg ggctgtcatt tgcgacgttt gtcgatccgg ccagccatct caatatgccc
    24301 gttttagcgc gtatcatgga tatgctggcg ttactgctgt tcctgacatt taacggtcat
    24361 ttatggttga tttcactgct ggccgatacc tttcacaccc tgccaattgg tggcgaaccg
    24421 ttgaacagca atgcgtttct ggcactcacc aaagcaggga gtttgatttt ccttaacggg
    24481 ctgatgctgg cattaccgct cattactctg ctgctgaccc tgaatctggc attaggctta
    24541 cttaatcgta tggccccgca attatccatt tttgttattg gatttccatt aattctgact
    24601 gtcggtatct ctttaatggc ggcattaatg ccgttaattg cgcctttttg cgaacattta
    24661 ttcagtgaga tgtttaattt gctggctgat attattagtg aattgccatt aatataattc
    24721 cgtaacgttt atcatgttat cctaaggatt atccgaaaaa taatacctac gaacatcttc
    24781 caggatactc ctgcggcgaa atatttgttt taagctcact cacatatcgc aacatttact
    24841 ttactttaag acaattccag gcaaattata caacacttta cgggatagta agtccgcctg
    24901 aaaaatcgcg agagtggcgc attaggtgac ccatgttgtt ccgtttagtc atgatgaaat
    24961 attcaggtaa ggggaattat cgttacgcat tgagtgaggg tatgccatgt caacgattat
    25021 tatggattta tgtagttaca cccgactagg tttaaccggg tatctgttga gtagaggggt
    25081 taaaaaaaga gaaatcaacg acattgaaac cgttgatgac cttgccatag cttgtgattc
    25141 acagcgccct tcagtggtgt ttattaatga ggactgtttc atccacgatg cttctaacag
    25201 tcagcgtatc aagctcatca ttaatcaaca tcccaatacg ttatttatcg tttttatggc
    25261 aattgccaat gttcattttg atgaatatct attggtcaga aaaaatttat tgatcagttc
    25321 taaatcgatt aaaccggaat ctctcgacga tatccttggc gatattctga aaaaagagac
    25381 aacgataacc tcgtttttaa atatgccgac gttatcattg agccgaaccg aatcgagtat
    25441 gttgcgaatg tggatggcag gtcagggaac cattcaaatc tctgaccaaa tgaatatcaa
    25501 agccaagacc gtttcatcgc ataaaggtaa tattaaacgt aagatcaaaa cgcataataa
    25561 acaggttatc taccatgtcg tccgactgac ggataatgtg actaatggta tttttgtcaa
    25621 catgcgctaa cacattctga ctggtggttt cccaccagtc aggccgaatt agattactct
    25681 gctttctcca caaagatacc gtcctgatgc cctgcttcat taaagaacca gatgccgagc
    25741 gggtagtctt ccagcgaaac caggtacatt gtgccttcac taaactcctc aactgccagt
    25801 accacgccag gacgacgcgg accgccatcc gttttgactg ttacccgatc attcaccttc
    25861 atcgttttcc tcctgtggct ttgtgccagt gtagaacaat ttcgtttttc tggcagcgcc
    25921 aggcgcgcgc gagtgctgat tttctcgacg gtctatactt aagagatgcc aggcggactt
    25981 aacgactggc cgcaacaaca gagtaacggt tgcgaggaaa gatgatgaaa accgcaaaag
    26041 agtacagcga taccgcaaaa cgtgaggtca gcgtcgatgt cgatgccctg ctggcggcga
    26101 tcaatgaaat tagcgaaagc gaagttcatc gcagccagaa cgattctgaa cacgttagcg
    26161 tcgatggacg tgaatatcat acatggcgtg aattggcgga tgccttcgaa ctggatattc
    26221 atgacttcag cgtctctgaa gtgaatcgtt gaatgcacaa taaaaaaatc ccgaccctga
    26281 gggggtcggg atgaaacttg cttaagcaag aagcacttaa aaaattcgtt acaccaggaa
    26341 atctgatgtg ttcatcacct tatccgcaat ttttttcgct gacaagaaaa tattcgtgat
    26401 atgaatgatt aacgctactt atgaatagaa atgtgacgcg cgtcacccct tggtggctct
    26461 ggcgtcaacg ggatcgctcc cgaaaaaatc atctttgata atttgtgctg ttttgagaat
    26521 catcctggta aggggagtac tgcgcaacca tgctttcaat tcaacaacca ctactggttt
    26581 ttagcgatct tgatggcacc ctgctggaca gtcatagtta tgactggcaa ccagctgccc
    26641 cctggctcag ccgtttacgc gaagcgaatg ttcccgtcat tctctgtagc agtaagacat
    26701 cagccgaaat gctgtacttg caaaaaacgt tggggctaca aggtttaccg ctgattgccg
    26761 aaaatggcgc agtgatccag cttgctgagc aatggcagga tatagacggt tttccacgca
    26821 tcatctcagg tattagccat ggcgaaatca gccaggtttt aaatacgcta cgcgagaaag
    26881 aacattttaa attcacaact tttgatgatg tcgacgatgc aaccatcgcc gaatggacgg
    26941 gattaagccg tagccaggcg gcgctgacgc agctacatga ggcgtcggta acgctaatct
    27001 ggcgcgacag tgacgagcgt atggcacaat ttaccgctcg tctgaacgaa ctgggcttgc
    27061 agtttatgca gggtgcgcgc ttctggcacg tcctggatgc ctctgccgga aaagatcagg
    27121 ctgccaactg gattatcgcg acctatcaac aatcgtcagg caaacgccca accacacttg
    27181 gcctgggcga tgggccaaac gatgcgccct tactggaggt aatggattac gcggtgattg
    27241 tgaaagggtt aaaccgtgaa ggggtgcatc tgcatgatga ggatccggcc cgcgtctggc
    27301 gaacgcagcg tgaaggaccg gaaggctggc gtgaagggct ggaccatttt ttctccgccc
    27361 attaagcgtt atcgctcgcg aatacccgat tgcgcccggc ctgtttagcg agataaagtc
    27421 gacggtcggc cagtgactgg agttgttcaa aatcataatc accggtttcc tcgctgctgc
    27481 ttacccccag cgaggcactg atgcgtatcg tcgtactctt ggcgatcaac atctcttttt
    27541 catttagctt caggcgaata cgttctgcaa cctccgcagc ctgcgtcagg ctcgcgcctg
    27601 gcagaatcac acaaaactcc tcaccaccga cccgcccggc aacatcctgc gctcgcaagg
    27661 aactgctaat taatccggca gcatgagaaa gaacacggtc gcccgcctga tgaccaaagc
    27721 ggtcattaat cgctttaaaa tggtcaaggt cgacctgaat gacagaaaaa ggatgttggt
    27781 gcgtctgaca caatttagcg agcggacggg ctttttcgaa cagtgcgcca cggttatata
    27841 aacgcgttaa ggtgtcgtgc cacgcctgcc agtgcaacga gctttgcaaa acatacatgt
    27901 tgctgaccat ccggcgaatc acataccagg agatgagtaa catggtggta aagagcgccc
    27961 acagcagggt taatgcaatg ctgatactgc cgaaatcgcc gcgcacgcct tcgcttagcg
    28021 tatggacgcg caccagcaca ccgtcgaaat ggtccagacg ttcccagcta acatagcgac
    28081 tgttcatacg aatgccgccc cgcgtgtcat gttccattgc ctgcgccagc aaggccagtt
    28141 cacgaggatc aaaaatattc cctgttggat ggtcaggatt ggaagaggtc aataatctca
    28201 gcttactgtc atagagctga tactcaccat cgaggttttt atcgatggcg tttcttaaaa
    28261 attgctgcat ggtacgcacg ggaatactca tccccagcac gccataccag tagttttcac
    28321 tgtctaccgg aacactgacg gtaacctgcg gttcagtatt gctggcgtgt tccggttgcg
    28381 aagtgaacca gcgtaccgcg cggtgacgat tttctcgttg cgaatggccg acaaaccagg
    28441 gttgggtgac atagccgtaa taacgcgttg gtacattgcg cgtaaacaag gtcggctgcg
    28501 tcgaaacgta aaatccggca cgcgagacat acatcgcctg ttcaaccatc gacgaggagt
    28561 tgtgtgccag tcgcagcaag taaccaactt ccagtgcagc ggtaatttca ttgtcgaggc
    28621 tttcattttc gcgagacagg agattcccct cgctgactaa tgcatccgac acaccattga
    28681 ctggcagggt gcgtcgtcgg ttgagttcga tttgccaggc gtgctcgtcg cgatgctgtt
    28741 cgaactcggt tacggcatta cgcagagagg tgaaatccag tggcgctacg agagcttcgc
    28801 gcatgccatt acgcaaaaag atcagtttgt cgacgttata ctgcaaatgc ttatcgagcg
    28861 cgttggctac gttttccaga tgattacgct ggctggagat ataggcatct tccagcacca
    28921 ccacttcacg ccaggtgagc aaggtggaaa aaagcaatac cacgataaag cagagattaa
    28981 cgacatgacc aggccccagg cggcgtgcga gttttttcaa ccagctctgg ttttccattt
    29041 ttgtctcgtg ctgcaccctg acgactccca acctgctttt ttatgattct ggcataacct
    29101 ggcgatagcg taccgcaaaa cagcgataat tttataatgg tggcatgata agacgcgaca
    29161 gacatcgata gttttataac gtaggcatga taagacgcga tagcgtcgca tcaggcaatt
    29221 cagtgcagac actggcacac ccgaccggat gcgacgtgag cgccacatcc ggtcgggtgt
    29281 taatgatacc ctttcaaact tcttccgcag aacgactccc ctcccccgga acaagctcgg
    29341 tttcatgaaa agcttcccgc ttgaccccgt aaccgtcata ccaacgacac tcaaccatac
    29401 cgctggagta tccggtgaca atcatccgcg ggccgccctc tttaaccgta acttcctcac
    29461 taaccataaa gctcatactc gcctcctttt ttcgagtgaa acttgttcac cttagttgaa
    29521 gatggcgaat tttgcataat agcctgtgcg agtattaatg tgcctgaccg cgtattttcg
    29581 ctacggcttt cactcccagc accacgatgc cgccgatgat aaatccaaga atcagattta
    29641 aaacagtcgg taatatcatc gccaccactg cactttgctg cccggcgaaa tgttcaatgg
    29701 cgtgatgcag cggcgcaatt ccatgtacca caatcccacc gccgacgagg aacatcgcca
    29761 gcgtgccgac aatcgataac gccttcatca accagggcgc aatgatcaat aaccctttgc
    29821 ctaatgcctg catcagcgcg ctggattttt ccgccagcca ataccccagg tcatcaatct
    29881 taacgataac ccctaccaga ccgtagacgc ccacggtcac taccagcgcg atgcctgaaa
    29941 gcaccagcac ctgattaagc aacggcgctt cggccacaat ccccagcgtg atggcgacga
    30001 tttccgcaga caatataaaa tcggtacgaa tcgccccttt tattttgtct ttttcaaact
    30061 tcagcggatc ctgcgccgcc agcttctcca gacgctgctg actctgcgcc ggatcttctt
    30121 tatgtttacg cgcctccagc atatgcagca ctttctctac cccttcaaag cagagaaacg
    30181 cgccgccaat catcaacagt ggcgtaatcg cccacgggat aaacgcactg atgatcagcg
    30241 ccagcggcac cagaatcact ttattgatca gcgatccttt cgccacgccc cagaccacgg
    30301 gaagttcccg gttggcgcgc acgcctgcaa cttgttgcgc attgagcgat aagtcatccc
    30361 ctaatacacc ggcggttttc ttcgccgcca gtttgcccat cacggagata tcgtccagca
    30421 gtgtggcgat atcatcgagc aacgtcagta aactactccc ggccaaaagc actccctctc
    30481 atttttattg ttgaagggtg taagtatgaa gcaaaaacgg cagctctgat acttgcttca
    30541 caggtcaaca tttatttaac aatcaagtgc aattatatct ctcgccagca gaatgatttt
    30601 cacgtttact atgaaccacc tttttatttc agccgtgagg gattatgcgt ttccgccagt
    30661 tgttaccgct ttttggcgcg ctgtttgcgt tgtatatcat ttggggctca acctattttg
    30721 tcattcggat tggcgtggaa agctggcctc cgttaatgat ggcgggcgtt cgattcctgg
    30781 cagccggtat tttattgctg gcatttttgc tactgcgcgg acacaaactt cccccgctac
    30841 gtccgctgct caatgccgcg ctgattggcc tgttattgct ggctgtcggt aatggcatgg
    30901 tgacggttgc cgaacatcaa aatgttcctt ccggcatcgc cgccgttgtg gttgcaaccg
    30961 tgcccctctt taccctgtgc ttcagccgcc tgtttggcat taaaacgcgc aaactggaat
    31021 gggtgggtat cgccattggg cttgccggaa tcatcatgct caatagcggt ggaaatttaa
    31081 gcggcaatcc gtggggcgcg attctgattt taatcggctc gattagctgg gcgtttggct
    31141 cggtttatgg ctcgcgcatt accttacctg tagggatgat ggcgggtgcg attgagatgc
    31201 tggcggctgg cgtggtgtta atgattgcgt cgatgattgc gggtgaaaaa ctgacggcgc
    31261 tcccttccct ttcaggcttc cttgcggtcg gctatctggc gctgtttggt tcgattatcg
    31321 ccattaacgc ttatatgtat ttaatccgca acgtcagtcc ggctctcgcc accagctacg
    31381 cctacgttaa cccagtggtc gcggtcttgc tgggtacggg gctgggtggc gaaacgctgt
    31441 cgaagattga atggctggcg ctcggcgtaa ttgttttcgc ggtggtactg gtcacgttgg
    31501 gaaaatatct cttcccggcc aaacccgttg tcgcgccagt tattccggat gcatcaagcg
    31561 agtaaatgaa tcccctgcgt gtcgatctgc gcgctggcac cttcgccgca gatccactct
    31621 tccagacgct cggtaagcgc cgcatccgtc agcttctcgc gcccgcgtaa cgcacattcc
    31681 cagacaatca atacgcgcca accgagttcc tgcaaatggc tgatatcacg acgatcgcgc
    31741 tcgacatttt tacctatctt ctccagccaa aaatctgttc gcgtggcggg gactttaaac
    31801 aggtagcaat ggtgatggtg ccagaagcaa ccgtgggcaa atatcacaca gcgaaattca
    31861 tcaaggacaa aatccgggcg tccgggcaga ctggcgtcct gaacgcgaaa cgccaggccc
    31921 tgcccagtta acaaactggc gaggcgcttc tcaatcgccg tatcacgcgt ggcaatcgcg
    31981 cgcatatttt tgctgcgggt ggccttatcg tgaacgtcga ccatgttgtg cctcttgctg
    32041 acgcaacgcc accgcctgtt tgatttttgg ctcaagcagt tttgccaccg cggcaaagac
    32101 cggcacgacc accgagttac cgaactggcg ataagcctga gtgtccgaaa ccggaatgcg
    32161 gaatttcgct tctcccggcg cttcaaaacc cattaagcgc gcacattccc gaggcgttaa
    32221 ccgacgtggg cgatgttgct gattcagcgg atcgtcaaag tctttctcac ccgttgccat
    32281 atcccagccg cgatcgatta aaatttccgc gccatctttg taataacgcg cagacagcgt
    32341 acgcgtgacg ctttgcggat tgttcggata aaccattcca taaccgaagc cgttaccgcg
    32401 cgcctgatgt tttttcgcat atcgatagag gtacttccac agtaccggcg tcaggatata
    32461 tttcgcctcg accatcgggt ccagcagctg cgccagcgtc actcgctgtg cagggaaaca
    32521 tttgctgata tcacgcagag taaaatcggc tttcagattc agatcgcgac gaaaacccac
    32581 cagcacgatg cgttcacggt gctgcggcag aaaatgtttg ccgtcgatga ttttcggatc
    32641 gtccggcccg ttatcttctg catcagccac gtcatagccc agttcgtcca gcgtctgcat
    32701 gatgatgcgg aacgttttac cctggtcgtg acttttcagg tttttgacgt tttcgagcac
    32761 aaacatcgcc ggacgacgcg cgtcgataat gcgcaccaca tcaaagaaca gcgtgccctg
    32821 ggtatcgcag gcaaaaccgt gcgcccgccc gagcgagttc tttttcgata cgccagccag
    32881 cgaaaatggc tgacaaggga aaccggccag taaaacatcg tgttcaggaa tgtgttgacg
    32941 aatatgttcc gccgccgcct catcactcac gccttcttga tggctgaggg tgatgtcgcg
    33001 gatatcctca ttaaaatgat gcgtcgccgg gtcgcaataa tggttggctt tataagtgcg
    33061 taccgcatgt ttgttccatt cgctggtaaa cacgcactgt ccaccaatcg attcaaaacc
    33121 gcgacggatg ccgccaattc cggcaaatag atcgataaag cgaaacgcat aatgcggatg
    33181 atgtgccggt ggtttgggta acaacgtttg cagatgggcg aactctttct cacttaaacg
    33241 gtgccatgcc gagtcattcg ccagcgcacg ttttaaaatt gccgcgctcc agtgattctc
    33301 acccacacca ttaagctgcg ccaccagcgt tttaacatca taaatttgca gcagtttctc
    33361 cagcattgcc tgtgcggcat tcccggtgct gtatgaatcg gttactgata tattttcctg
    33421 catagattta accggccatc tgagatggcc gacaggttaa cataattact gccgtttagg
    33481 gccacgcgct cagccagcag gtgaaaacgc atctatcacc ttgtgatcga taccttcatt
    33541 ctcccccgcc agttcggcac cgagcttcgc cataaactcg actaaaaatt gcgcgttgtg
    33601 ctgtgccagc tgcttgcctc ttgcggtttg catggtttgc ggtaatttca gtaactttgt
    33661 ctggaaatga tccagcgcgt agcgtttatc atcaagcggg cgatgctgtg cgaacggatc
    33721 ttcgccatca aacagcgcca agcccaatgc ccctgaaacg gcaaatacgc gcgccaggcc
    33781 aatcgccccc agggcttcca gccgatccgc atcctggacg attttagctt ccgttgttaa
    33841 gggggcgatt tgcgcgctga aactgtgagc ggcaatggca tgacaaacgg cctcaatttt
    33901 ctccgccgga aattgcacga act
//
LOCUS       DAZGJW010000039        33582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZGJW010000039 DAZGJW010000000
VERSION     DAZGJW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.039"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(30..911)
                     /gene="prmA"
                     /locus_tag="ACHKIK_001898"
     CDS             complement(30..911)
                     /gene="prmA"
                     /locus_tag="ACHKIK_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO9083081.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(923..2374)
                     /gene="panF"
                     /locus_tag="ACHKIK_001899"
     CDS             complement(923..2374)
                     /gene="panF"
                     /locus_tag="ACHKIK_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO9083082.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2364..2606)
                     /gene="yhdT"
                     /locus_tag="ACHKIK_001900"
     CDS             complement(2364..2606)
                     /gene="yhdT"
                     /locus_tag="ACHKIK_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO9083083.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2699..3634)
                     /locus_tag="ACHKIK_001901"
     CDS             complement(2699..3634)
                     /locus_tag="ACHKIK_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO9083084.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            complement(3666..4496)
                     /locus_tag="ACHKIK_001902"
     CDS             complement(3666..4496)
                     /locus_tag="ACHKIK_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO9083085.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            4838..5692
                     /locus_tag="ACHKIK_001903"
     CDS             4838..5692
                     /locus_tag="ACHKIK_001903"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HFO9083086.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            5728..6618
                     /locus_tag="ACHKIK_001904"
     CDS             5728..6618
                     /locus_tag="ACHKIK_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO9083087.1"
                     /translation="MKNIKLLGSAMLVSLALFSQNSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLNGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            6679..8178
                     /locus_tag="ACHKIK_001905"
     CDS             6679..8178
                     /locus_tag="ACHKIK_001905"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO9083088.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            8179..9168
                     /locus_tag="ACHKIK_001906"
     CDS             8179..9168
                     /locus_tag="ACHKIK_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9083089.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFILLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGISGMILASRRYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            9190..10152
                     /locus_tag="ACHKIK_001907"
     CDS             9190..10152
                     /locus_tag="ACHKIK_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO9083090.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            10149..11195
                     /locus_tag="ACHKIK_001908"
     CDS             10149..11195
                     /locus_tag="ACHKIK_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO9083091.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGIVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDGIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVLRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSVTPRTP
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEMSALKAEIAGYR"
     gene            complement(11287..12636)
                     /gene="accC"
                     /locus_tag="ACHKIK_001909"
     CDS             complement(11287..12636)
                     /gene="accC"
                     /locus_tag="ACHKIK_001909"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO9083092.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(12647..13117)
                     /gene="accB"
                     /locus_tag="ACHKIK_001910"
     CDS             complement(12647..13117)
                     /gene="accB"
                     /locus_tag="ACHKIK_001910"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO9083093.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPIMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(13983..14813)
                     /locus_tag="ACHKIK_001911"
     CDS             complement(13983..14813)
                     /locus_tag="ACHKIK_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083094.1"
                     /translation="MTSLFNRLTGKAVSRTAFVEHLGQEVVQHHPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLQEVAADMLKQLAEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEVLNASLDFAWEDFNDELIILFAEDGSETMRYLMRYEVTIDREQ
                     AISNLKQILPEVTAEIVDTGANDGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGDLL
                     VTVPSRGKLFFTHNDQPANLSALRQLTKQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            complement(14850..15824)
                     /gene="yhdH"
                     /locus_tag="ACHKIK_001912"
     CDS             complement(14850..15824)
                     /gene="yhdH"
                     /locus_tag="ACHKIK_001912"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO9083095.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            15976..17916
                     /gene="csrD"
                     /locus_tag="ACHKIK_001913"
     CDS             15976..17916
                     /gene="csrD"
                     /locus_tag="ACHKIK_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO9083096.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            18221..19264
                     /gene="mreB"
                     /locus_tag="ACHKIK_001914"
     CDS             18221..19264
                     /gene="mreB"
                     /locus_tag="ACHKIK_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO9083097.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            19330..20433
                     /gene="mreC"
                     /locus_tag="ACHKIK_001915"
     CDS             19330..20433
                     /gene="mreC"
                     /locus_tag="ACHKIK_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO9083098.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            20433..20921
                     /gene="mreD"
                     /locus_tag="ACHKIK_001916"
     CDS             20433..20921
                     /gene="mreD"
                     /locus_tag="ACHKIK_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO9083099.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            20930..21523
                     /gene="yhdE"
                     /locus_tag="ACHKIK_001917"
     CDS             20930..21523
                     /gene="yhdE"
                     /locus_tag="ACHKIK_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO9083100.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            21513..22982
                     /gene="rng"
                     /locus_tag="ACHKIK_001918"
     CDS             21513..22982
                     /gene="rng"
                     /locus_tag="ACHKIK_001918"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO9083101.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            23050..26850
                     /gene="yhdP"
                     /locus_tag="ACHKIK_001919"
     CDS             23050..26850
                     /gene="yhdP"
                     /locus_tag="ACHKIK_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO9083102.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTSVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            27006..28451
                     /gene="tldD"
                     /locus_tag="ACHKIK_001920"
     CDS             27006..28451
                     /gene="tldD"
                     /locus_tag="ACHKIK_001920"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO9083103.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNCR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(28579..29508)
                     /gene="aaeR"
                     /locus_tag="ACHKIK_001921"
     CDS             complement(28579..29508)
                     /gene="aaeR"
                     /locus_tag="ACHKIK_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO9083104.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            29691..29894
                     /gene="aaeX"
                     /locus_tag="ACHKIK_001922"
     CDS             29691..29894
                     /gene="aaeX"
                     /locus_tag="ACHKIK_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO9083105.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            29902..30834
                     /gene="aaeA"
                     /locus_tag="ACHKIK_001923"
     CDS             29902..30834
                     /gene="aaeA"
                     /locus_tag="ACHKIK_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO9083106.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            30840..32807
                     /gene="aaeB"
                     /locus_tag="ACHKIK_001924"
     CDS             30840..32807
                     /gene="aaeB"
                     /locus_tag="ACHKIK_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO9083107.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     SMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            32899..33171
                     /gene="yhcO"
                     /locus_tag="ACHKIK_001925"
     CDS             32899..33171
                     /gene="yhcO"
                     /locus_tag="ACHKIK_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO9083108.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(33227..33490)
                     /gene="yhcN"
                     /locus_tag="ACHKIK_001926"
     CDS             complement(33227..33490)
                     /gene="yhcN"
                     /locus_tag="ACHKIK_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO9083109.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         8210 a         8113 c         8924 g         8335 t
ORIGIN      
        1 ccccgcgtca ccctacggcg atgcgaaggt taattcttac gaccggtaat acggcaccac
       61 tcttcttttt ccacgaccgg gtccagtgcg aagctatcgg cataagcttc acaaacgctc
      121 tctgcctggc ttgccagaat accggaaagg cccagcaaac cgcctgaaac cggcaggacg
      181 ctgattaacg gtgccagttc acgtaatggg cctgcaagga tgttagcgac caccacgtcg
      241 gctttcattt cttctggctg atctttcggt aagtagagtt ccagacggtc agaaacgcca
      301 ttacgttcgg cgttgtcgcg gctggcctga atcgcctgcg gatcgatatc aataccaatg
      361 gcttttgctg cacccagttt cagcgccgcg atcgccagaa tgccggaacc acagccaaag
      421 tcgatgactg ttttaccggt taaatcgagg ctgtcgagcc attgcaggca cagagaggtg
      481 gttggatggg tacccgtacc aaacgccagc cctggatcta acatcacgtt gacggcgttt
      541 tcgtccggca catcacgcca gctagggcag atccacagtc gttcaccaaa gcgcatcggg
      601 tggaaattat ccatccattc gcgctcccag tctttatctt ctagttgttc gattttatgc
      661 gcgaagcctg cgccgagcag cggatggttt tccagaatcg ccaccacgtc gttcatatcg
      721 gtttcagcgt cgaacagacc aatcacatcg gtgtcgcccc acaggcgcgt ttcgcccggc
      781 agcggctcaa atactggcgt atcgtgggta tcctgaaaag tgatagaaac ggcacccgct
      841 tccatcagcg catcgctaag atcttccgcg ttcgcgccgg tggtgttcag tttcagttgg
      901 atccaaggca tggcaaaact ctttatttat cagtagtcaa aacggtagct tgcgggacgg
      961 atgtaccgaa acggtttccg accaggaaag ccagcaaact tagtagtaac gagggcacaa
     1021 tagggtggaa gcccaggtac tgaatattca gcgtcgcgag tacggcatac agcacgccgc
     1081 caacgatcat cgcacttagc gcgcctttgg cgttggcgcg ttcccagtaa agacccagca
     1141 ccagcggcca caggaaaacg gcttccagcc caccgaaggc cagcaaattc agccagatga
     1201 tcatttctgg cggcttccag gcggcaagca gcagcaacgc gccgagaact aacgtaatta
     1261 ccgccgacat ccgcttcaga cgcgtctcgt tttgcatttg atccggacgg atattcagat
     1321 agagatcttt aatgatcgta gcggaacttt gcagcaactg ggcattgatc gtcgacatga
     1381 tcgcagccat aggtgcagcc aggaagattc cggcagcaaa cggtggcagc acttttacca
     1441 ttaacgtcgg gatcaccaga tccggtacgg tgagatcggg gatcactgca cgacctaacg
     1501 ctccggccag gtgcataccg aacatcagga ttgcgactac aatcgtaccg atgatgatcc
     1561 cccggtgtac tgctttgctg tctttataag agatacagcg caccgcagta tgcggcaggc
     1621 caatcacgcc aaagcacacc agtacccaga acgacgtcat aaaggcaggc gacagaatat
     1681 cgtcagcgcc ttgtggcgta accagttgcg gatcgatggt ttgcaaggtc tgtactgcgt
     1741 tgcttaagcc gccagcggca tgtaccacgc caataagcag cacaacggtg ccaatcagca
     1801 tcacaagccc ttgcatggtg tcgttcagca cgctggcgcg aaagccacca aaggcggtat
     1861 ataacgcaat gctgatacca aaaatcagca gcccggtttc ataaggaata cccgccgcgg
     1921 tttccagcag gcgcgcaccg ccgataaact gcacggtcat cgcaccaacg aacgcaacca
     1981 gcaaactcaa actcgccagc cacaccagaa gacgactctg gtagcgggca aacagcatat
     2041 cgttcagcgt cactgcattg tagcggcgcg caagaatcgc aaacttcttg ccgagaatac
     2101 cgagtgaaag ccagactgca ggaagctgaa tcatcgccag caatacccag cccagcccgt
     2161 atttataagc agctcctggc ccgccgataa acgaactggc actgatatag gtcgcggtga
     2221 gcgtcatcgc cagcacaata ccgcccatag agcggctgcc gaggaaatac tcattaagga
     2281 aggtgccggt gctccgttta cgcatcgcat aaaccgagat accgaacacc accaccagat
     2341 aggcgaccag cggtagaatt acttcaagct gcatcgtcat cctccagtgg gatatcgcga
     2401 tagataaatt tcaccatcgc ccagcacagt ccaataaaca gcagcggcgt caggatgcag
     2461 gccatctcaa accagcgcgg aaagccggta aaaccggggg caacgccagg taagtaagcg
     2521 gctactaacc aaactgccag atacaaaagg gtcagcccca gcgcccagcg cgcctcttta
     2581 tgggcctgaa caaaacgagt gtccacggaa gggttcctcc tcatgggggg atacgagaaa
     2641 cgggcaattc tacggcaact cttcaggcag ggaaaataat ctgacagaaa attgccgttc
     2701 agtgctgctg caaaaagtct tcaatgtcag ccagtgagga tatcccttcc atcggacctt
     2761 gccgcattac ggcgagcgcg ccgctggcat ttgcatattg cagtgcctta tgtgccggga
     2821 aaccggataa gaaaagagtg ataaacgttg cgccaaagca gtcgcctgca cccgttggat
     2881 cgataatttc gatatcgtgg cctgtaacat gctgggcgtg taatgtaccg tttttaagct
     2941 tgtaatagct ggccccacgc tgggcgcgtt ttatcgccac atgttttacg ccgccgtgga
     3001 gaagatcgct aacaatctgt tcttctgaca gatttttgtg acgcgcgaag aaagggagtt
     3061 cgctttcgct ggggatgaaa atatccgtat attcaatcaa ataatcgaga gcctgcgcca
     3121 tttcaggaat gctcagcatc tctttgcgaa tattgggatc gaaagaaacg gtgccgccag
     3181 ccgatttgat cgtcgttatt gctttacgca tgacatcgat catgcgaaat gagaacaacg
     3241 atgagcccac aatatggaga tggttacact gttttagcag atccttatca atgtgctcag
     3301 cagtaaacaa gccgcaggcg ctgttaggga tattaaagac gaaatcgcgc tgctggggat
     3361 tttgataact cacgaacgcc gtacctgtga cggcattgtt cataacatgg atcccatcgg
     3421 taatgacacc ttcatgacgg aggcgctcta taatgagtcg gccaaaatca tcattaccaa
     3481 cacatccgaa gaagatggcc cgatgggaca gttttgccac ctgagcagca aaaattgctg
     3541 gcgcaccgct gggaaatggc ccgataaact ccccaggctg agtgaatttt tgatgtgggt
     3601 tgcgggacaa aaactcggcc agcaattcgc ccatagcgca gatagttatt gtcacaaaac
     3661 ccctctcatt cctgaagcct ttcgcgattg gaccaggtaa agcactcatt actggtataa
     3721 aggataacga aatcagccga ctgcaattgt tgctgatatt gttcgttaat gctgcggctg
     3781 acgatcacat tttctgcctg atgcaaatgc ccgatagcag ccgttgatgg cgcaggatcg
     3841 gcggcaacga taaccgtctt attctgggca tgatcgaaac agagctgcca ggcagcggcc
     3901 agtctgggaa cgttgatatt taaagtacca tcagcatcca gatggctgat ttccagaatg
     3961 ctatgtgtaa tggtgaaatg ctgaagtgcc tgttcaagcg gttttccggc cagtgcggtg
     4021 tcggggcgca gcatttcact gccagccagt attatttcgc catcaataaa ctgatttgcc
     4081 agcagcgcat gttcgggatc gttggtgatc agacgcagtt ttttcacttc gctgagcaat
     4141 ggaatgatct tacggcagat tgtcccatgt ccaagaaata gcgtgtcacg atcgcgaatt
     4201 aaccgtgcgc aatgtcgtgc tatttccatt tgttgtgcca gagacaatgg cgcgatcggt
     4261 tgtatcgctt ctggttcaga ggagagtggt gtcgcaatag caccgccaaa tgatcgcttt
     4321 aaatatccct gttgctccag ataactgaga tcgccgcgga tcgtcacgcc agagacacca
     4381 agttgcgcag ataagtcatc aactttgatt tcaccttggg cggcaacaat ttctgcaatt
     4441 tttaatctgc gtttcagtag gttagacgat tttctcggca cgttaacact cttcattggc
     4501 ctttaatgcg taacactata gccccttgta tttttctttg aaagttatcc ttcaataata
     4561 tcgacaattc gaaaagaaaa tcatgggcca aaaatcgcaa caaaaataga ttaattcatt
     4621 gagaaataaa tgaatttatt catatttatg cagagatgtt attggtttca cttgtaaagt
     4681 gaaaaacaaa ttaagttatt ttttgtaagt tattttgatg atattcgaca tgcatcacgc
     4741 tttttgccgc ttttgctgtg ctagtttgct ttcaaaatga aagtgaatat tctctttctt
     4801 cattttgtaa ggcgaacgac aaataacagg agctgcgatg tacatcattt caagcaaaaa
     4861 catgctgaaa aaagcccagt cagagggata tgccgtcccc gctttcaaca ttcataacct
     4921 tgaaacttta caggtagttg ttgagagcgc tgcacaactt cgctctcccg ttatgctggc
     4981 gggaacaccc ggtacttatc gttatggtgg cgtgggaagt ttaatttcgt tagtacaatc
     5041 gctggctcgc gagtacaacc tgccgttagt cctgcatctc gaccatcatg aagagagtga
     5101 tgacattttt aacaaggttc gcgcagggat ccgttctgtc atgattgatg gctcccattt
     5161 gccatttgaa gacaacattg cgcgagtgca agaagttacc accttttgcc atcgctttga
     5221 tgttagcgtt gaggccgagc tagggcgtct tggcggccag gaggacgatc tgctggtgga
     5281 tgaaaaagat tccgcttata cctcaccagc cgcagctgca gagtttgttc aacgtaccca
     5341 aattgactcc ctggccgtag caatcggcac agcacatggt ttatatgcgc atgaaccacg
     5401 actggatttt gtgcggctgg aacagattcg tcagcgagtg gagattccac tggtgttgca
     5461 tggtgcttcg ggcttatcag aacaggacgt tcagcactgt attcgccgcg gtatttgtaa
     5521 agtgaatgtc gcgacagaat tgaaaattgc gtttgccgat gcgttaaaag gatatttcta
     5581 caacaatcct gcggcaaatg atccacgcca ctatatgcag cccgcaaagg ccgccatgaa
     5641 agaagtcgta actcgtatta ttggtgtctg tggtagtgaa ggaaaaattt aagacgttgt
     5701 accctacaaa aacaatgagg attgtttatg aaaaatatta aattacttgg ttcagctatg
     5761 ttggtgagcc tggcattgtt ctcgcaaaat tcgttggcaa aagagtataa aattggtgcg
     5821 tcattattaa cacagcaaca tcctttctat attgatcttg cgaatgccat gaaagaagag
     5881 gcaaaaaaag acaatgtcaa tttaagtgtc tctattgcca atcaggattt aaacaaacaa
     5941 ctttctgatg ttgaggattt tattactaaa aaagtggatg ccattattat ctctccggtc
     6001 gattcgaaag gcgtccaggc ggcgataatt aaagccgata aggccggtat tcccgttatc
     6061 accgtcgacg tcgctgcgga aggcgttccg gtcgtatcgc atgttgcgac cgataactat
     6121 gcgggaggcg ttgaagcagg caagttaatg ggcaaacttt tgaatggtaa aggtactgta
     6181 gccattattt cttatccggc actacaatcg gtggttgcgc gcgttgatgg ttttaagaaa
     6241 gggttaagcg atacaccgga tattaaaatt gttgctgaac agcctggtat tacgcgggct
     6301 gaagcgttaa cgactgcgca aaacattatg caggcaaatg ccaacctgaa tggtctgttt
     6361 ggctttggtg atgatgcggc gctggcagcg gttatcgcag caaaatctgc gcataacgac
     6421 aacatcaaaa tcattggttt tgacggcatg aaagaagcgc gtgatgccgt tgacagtgaa
     6481 aaaacgtttg cggccgtgat tcggcaatat cccgatcaaa tgggtgctaa agccattgat
     6541 gccgctgttg atcacctcaa tggtaaaccg gtagaaaaaa tgatcccggt acaacccggc
     6601 gtttatacag gtaaatagta aacatcttca cctcacaaca aaacgttatt ggcgttttgt
     6661 tgtgacaccg aggtcattat gacggatgtt attcttgatg tttcgcatat tgcgaagaca
     6721 tttggtcatg ttcaagcatt aaaagatatt accctctctt tacgtaaagg gcgggtgcat
     6781 actttattag gcgagaatgg tgccggtaaa tcgacattaa tgaaaatact ggctggtgtt
     6841 tatccgccaa cccagggaac gataacacta cgtggtgaaa ctatcaccat taataatccg
     6901 cagcattcac gccagttagg aattgcgatt atttttcagg aattgagcct gagtaataat
     6961 atgaccgtgg cggaaaatat ttatgccaat aatgaaccgc gacgttttgg cattattaac
     7021 gataaaaaaa tgctcgcaga ttgccagaat ctcctcgcgg aactgggcat accactagac
     7081 ccactagaaa tggtcggcaa tatgtcgatg gcacataggc aactggtaga aattgctaaa
     7141 gccttaagct atgcagcgga tgtcgtgatt atggatgaac cgacatcttc gctaagtgat
     7201 aatgaagcgg agattctgtt taacattatt gaaaaattaa agcagcgagg ctgtgcggtt
     7261 atttatatct ctcatcgcat ggatgaaatt atgcggattt cagatgatat ttcggtgatc
     7321 cgcgacggtg agtatatcgc cacgcatgaa aagaaaaaca gtgatatcca gcatttgatt
     7381 gcccaaatgg ttggtcggga aatgaaaaat atttggccag cacgtcttgg ggaaataccg
     7441 gatgaaaatg ttcctgcaaa actggaagta aaaaatcttt cccatccttc gctttttaaa
     7501 gagatcagtt tcgctgtgcg tccgggggag gtcctcggat tttttggcct ggtgggggcc
     7561 gggcgttccg atgtgatgaa ggcgctcttt ggcctcgttt cgtatcaggg gactgtgttg
     7621 attgatggta aggaagtcag gatcgctaat cctaaacagg ctattgatca tggcattgct
     7681 tttgtgaccg aaaaccgcaa ggaagaaggg ttagtgctga tgcacgacgt caatatgaac
     7741 acgcaccatg tggcgtttca atataacgct tcgcggatgg gactcatcaa tcaccgacaa
     7801 gaagaggcga agacgctgca atcaattgcc agaatgaaca ctaaagtgag ttctgtacat
     7861 caggcagtgg gagcgctgag cggcggtaat caacagaaaa tcgtgttatc gaagtggctg
     7921 gagaaaactc cgcgtatttt attgctcgat gagccaacgc gcggagtgga tgtcggcgca
     7981 aaatttgaaa tctataacgt gatacgtcaa ctggctgcgg cagggacagc aattattctg
     8041 gtgtcctctg aactgccaga agtcatggca cttagcgatc gtctggtggt aatgcgtaat
     8101 aaaacgattg ccgatattta ttcatgtgaa aatttaacgc agacacaggt aatgaccgct
     8161 gcaacaggag tacgttaaat gacagagaaa actcttgcca gtccgaaacc ttcggaaatt
     8221 cccattgcaa ataattttaa aaaagtcttc aggcaatatg gcggtatcct cagtggaatg
     8281 gtggtgttaa tcattctgtt cagttttatt aatgacagtt tttttaccgc caacaacatt
     8341 actaatatta ttcttcaggt ttcgataatt gcaattaccg cctatggaat gacttatgta
     8401 cttttgcttg gcgatattga tttatcggtc ggttcaacca ttgcattgat tggcacattt
     8461 gctgcgcttg gggcttcatg gggaattcca tttattttat tggtcccatt atccattatt
     8521 gcagcactgg ctctggggat gataaatggt ggcttgacgg ctatagcggg gatcccgtcg
     8581 tttattgtca ctgttgctac tatggggatc ttccgtggta tcgcttatat agtgaccgat
     8641 ggtatgccca tcatgattaa agatgatgcc tttctggcac tggggaatgg agaatttctc
     8701 tatataccta ttccaatctg gatattaatc atcttattgc tgattaacca cttcattctt
     8761 actaaaacaa cctttggacg aaaaatttat atcactggtg gtaataaaga agcggcaatt
     8821 tattcaggga taaatgtcac ccgcttgaaa attaaagtct ttatgattac agccgtgctg
     8881 gcaggtatca gcgggatgat cctcgcctca cgccgctatt ccgggcaacc gaattccgcg
     8941 ttgagctacg aactggatgc tattgctgcg gctgtactgg gaggcaccag cctgaacggc
     9001 ggctatggaa ccgtggtggg gacggtgatc ggcgcattaa ctatcggcgt tattaataat
     9061 ggcatgaact taatgaatgt tccctatttt taccagatgg tggttaaagg attagtcatt
     9121 cttgtagccg tttattttga cgtaagaaat aaaagaaaac gcagctaatc atttcaagga
     9181 attaatcata tgagtaaggt cttcacaatt ggagaaattc tggttgaaat tatggcgagt
     9241 aaaatcggcc agccatttga tcaaccaggt atctggaacg gcccttatcc cagtggtgcg
     9301 cccgctattt ttatcgacca ggtgacacgt cttggcgttc cgtgcggcat cattagctgt
     9361 gtggggaatg atggctttgg tgatattaat atacatcgtc tggctgctga cggtgtggat
     9421 atcaggggga tttcagtctt acctctggaa gctaccggga gtgcctttgt tacttatcat
     9481 aactctggcg atcgcgattt tatttttaat attaagaatg ccgcttgcgg taaattatcg
     9541 gcgcagcatg ttgatgaaaa tattctgaag gactgtaccc attttcatat tatgggctct
     9601 tccctgtttt cgtttcatat ggtagacgca gtaaaaaaag cggtaactat cgttaaagca
     9661 aatggtggtg ttatttcttt cgaccctaat attcgtaaag agatgctcga tattccagaa
     9721 atgcgtgacg ccctccactt tgttcttgag ttaacggata tctatatgcc cagcgagggc
     9781 gaagtgttgc tcttgtcgcc acattcgacg ccggagcgtg caatcgcagg ttttcttgaa
     9841 gaaggtgtta aagaggtgat tgtgaagcgc ggcaatcaag gtgccagtta ctactccgcg
     9901 aatgagcagt ttcatgttga gagctatccg gtggaagaag ttgacccgac cggtgcggga
     9961 gactgctttg gtggcgcgtg gattgcctgc cgccagctag ggtttgacgc ccatcgagcg
    10021 ctgcaatatg ccaatgcctg cggtgcgctg gcggtaacca gacgtggccc aatggaggga
    10081 acgtctcgcc ttgcggaaat cgaagcgttc atccagcgcc atgacatgtc cattcgggaa
    10141 gcagcacaat gaaaaacgaa atttggtcgt ggattggcga tctctcgcag gttgccggaa
    10201 ttaaacacta tgagttacgt agtggcagag caaagggtac agaagcgttt gatgtccgta
    10261 ctggtgcggg actggcattt acggtggtaa aagatcgcgc gctggatatt gcatgggcca
    10321 gctacaagga cacggcgctt tcttttatca cccccaacgg cattgtcgcc cccgcttttt
    10381 ttgagtctca gggcaacgga tttttacgta gcttttacgc ggggttactg actacctgcg
    10441 gacttagcta cattggcacg ccttgtgagg atgaaggaga aacgctgggg ttacacgggc
    10501 gtttggctgc gactccagca gaagaggtgg ggtatcgcac agaacggacc gacgacggca
    10561 ttgaatttgt catcaatggc aaagtacggg aaacgcgact gttcggcgaa aatctgacgc
    10621 tggaaaggac cattcgctgc cgttatggcg aaaatgtgct gcgcattgaa gacaaggtga
    10681 ctaatcatgg tttcacccgc cagccgctgc aaattcttta tcactttaat tacggctggc
    10741 ctttattatc gccacaggca gaaatcttgc tgtcggctaa aagcgtaacg ccacgaactc
    10801 cgcatgcggc ggaagggtta accagtcatc tggagatttg taccccacag cccggttttg
    10861 acgagcaggt ttactatctc acgttgaata gcgacagtca ggggatgagt aaagttgctc
    10921 tggtgaacgc cgaactgggg tggggaattt acgagaaatt cgacaccatg cagctaccca
    10981 actttatcca gtggaaaaat ttaggcgcgg gtgagtatgt tatgggactg gaggtcagta
    11041 atagttttcc tgacggtcgt gataaagaac gagcgcaggg gcgtctgccc tttattgagc
    11101 caggggaaac aaagaaatac tgctttgagc tgggaattgt tgatggcgat gctgaaatga
    11161 gcgcgttgaa agcggaaatt gcaggctacc gttagctaac ggcaataaaa acgccgggca
    11221 gcgttaacag gcccggcgtt gattacgttc tttgctaagc gcgatgaaaa caccgcgcgt
    11281 cacaaattat ttttcctgaa gaccgagttt tttctccaga tagtggatgt tagtgccacc
    11341 atgctggaag ttctcgtcat tcatgatgcg gatctgcaga tcaacgttgg ttttgatacc
    11401 gtcgatgatc agctcctgca gcgcattctt catgcgggca atcgccacgt cacggttttc
    11461 accgtagcaa atcagcttac cgatcattga gtcatagtac ggcggtacgg tgtagcccgc
    11521 gtagatatga gactcccaac gtacgccaaa accgccaggt gcgtggaaac gggtgatttt
    11581 gcctgggctt ggcaggaagg tgttcggatc ttcggcgttg atacgacatt ccaccgcatg
    11641 gccgcgaacg tgaacttctt cttgcttgat cgacagcggt tgaccggcag cgatacgcag
    11701 ctgttctttg atcaggtcaa cgccggtgat catttctgta accgggtgtt ctacctgaat
    11761 acgggtgttc atttcgatga aatagaactc gccgttttcg aacaggaact cgaaagtacc
    11821 tgcaccgcga tagccgatat caacacacgc tttagcgcaa cgttcgccga tgtagcgacg
    11881 cagttccggg gtaatgcccg gtgctggtgc ttcttcgacc actttctggt ggcggcgctg
    11941 catggagcag tcacgttccg ccagatagat agcgttgccc tgaccgtcag ccagtacctg
    12001 aatctcgacg tggcgaggat tttccaggta tttctccatg taaaccatat cgttgctgaa
    12061 agcagctttc gcttccgcac gggtcatgga gatggattgt gccagttcag cgtcgccgcg
    12121 cactacgcgc ataccgcgac caccgccgcc gccggaggct ttgataatca ccggataacc
    12181 aatgcgttta gcaatggcac ggtttttatc catatcgtcg cccaacgggc cgtcagaacc
    12241 cggtacgcaa gggacgcccg cttttttcat cgccgcgatt gcggatactt tgtcgcccat
    12301 caggcgaatg gtttctgctt tcgggccgat gaagataaag ccggagcgtt caacctgctc
    12361 ggcaaagttg gcgttctcgg agaggaagcc gtaacccgga tggattgcta ctgcgccggt
    12421 gatttcagcg gcgctgatga ttgccgggat gttcagataa ctttttactg acggagcagg
    12481 gccaatacag accgtttcat ctgccagtaa tacgtgtttt agatcgcgat ccgcgctgga
    12541 gtgcacagcg acagtcttga tgcccagttc tttacaggca cgaagaatac gcaatgcaat
    12601 ctcgccgcgg ttggcaataa caattttatc cagcatgttc gcctcgttac tcgatgacga
    12661 ccagcggctc gtcaaattct accggttgtc cactttcgac cagaattgct ttcacggtac
    12721 cggatttgtc cgcttcgatc tggttcatca ttttcatggc ttcaacgatg cacagggtat
    12781 cgcccacgtt gactttctga cccacttcga tgaacgcttt tgcgtccggg cttggggtgc
    12841 ggtagaaagt accaaccatc ggggaacgta cgatgtgacc actgatttcc gctgctgctg
    12901 gcgcttccat ggaaggaacg gtcgccggag cggctgcgtt agattgagct ggctgctgca
    12961 ttattggtgc agcgtaagct tgttgcatca cagggaaact tgcggcagga gctgcacggc
    13021 taatgcgtac tgactcttcg ccttcagaaa tttccagttc ggagatgcct gattcttcaa
    13081 ccagctcgat cagtttttta atcttacgaa tatccatgag tgggttccgt actctttgtt
    13141 tagtgtgatt gtgacaggcg tttcaccgcc gtctgtaaag cgtatgcccc gacagctgta
    13201 tgcatagcga taaattccag caggccggga cgccggtcta ttttgcccca gagtgtcaat
    13261 gttagacttg acggacattg tgcagcgatc tgctgctatc ctccggcaaa aaacaaaata
    13321 taccttcgtt gcgccaatgt caccttttcc gcagcgcaaa aactgcgtca gggaggacga
    13381 ggtcgcacat tataacgatt tcgtagcaat tggcagctaa atactggtct tatcagggaa
    13441 gataatcaac agctaacatg taaataacct tcaacaccgt gtaatttgca acaagccgca
    13501 caattcacga aattagcgcc atcatcgacg gaacttctta taacgtaagg tgaaaagctg
    13561 taaagccagc cctgcgaaga tgaggggctg caatgagata atcttcacag accacaaata
    13621 atgtagggat gccaggatcg cgacaagata gacgacatta cgcagcggtt gctggggatt
    13681 acccaatttt cactgcattg tctggataga tgttaatgct aaagcacgca aaattaaccg
    13741 attgacaatg ctcagcatta aataggttcg agtgattaat ttgcagtcaa acaaaatcag
    13801 attattcccc ccattttgca gaaggtaaac tgttttgtca ggcggcagtg cggctatctg
    13861 atgctttcag aatttgccac ctgacaataa aaaagccgat ttggtagcaa aatcggcttc
    13921 ggtgagtttg cagttttcat cacatactcc gacaagcttt ttcaagcatg acggaggttg
    13981 cgttaatgca aggtttctac ccagggataa aagtgaccgt tacggcggat gaatatctgt
    14041 gttgtcagag gatggtcatc ctcacggaag cattgttttg ttagctgtcg taatgcactg
    14101 agatttgctg gctgatcatt gtgggtgaaa aacagttttc ctctggaggg aacggtcact
    14161 aacaggtcgc cgcattcagg atcgggtgat tgcacccaaa gatcgtcaat gagcatcaga
    14221 ctgggctcat aagtaccccc tgcggagata acggcaactt ttccatcgtt tgcgcctgta
    14281 tcaacgattt cagccgtaac ttccggcaat atttgcttca aattactgat ggcttgttcg
    14341 cggtcaatgg tcacttcgta gcgcatcaga tagcgcatgg tttcgctacc atcttcagca
    14401 aacaagataa ttaactcatc gttaaagtct tcccaggcaa aatccagcga ggcgttgagg
    14461 acctctggat cagactcata ctggctaagc tgttggcgaa cggtatccag ccaggctttt
    14521 tctttaatca ccggaacaat acagcgcccg gtcggcagca tttctgcaag ctgtttcagc
    14581 atatctgcag ccacttcttg cagcgggtct ggtgcggctt gataacgttt gtaggcatca
    14641 tcaagggtga tttccaccgt ttggtgaccc gtatcaatgc gcatcaattt gtggtcagtc
    14701 gaaatcatga ctttccagtt tggatgatgt tgtacaactt cctgaccaag gtgttcgacg
    14761 aaagctgtcc ggctaaccgc ttttcccgtt aaccgattga ataatgatgt caaaatgagt
    14821 tccctctctt attattcctt gctaaatggt tagttaacct tcaccagcgt gcgaccctgg
    14881 atctggttat taatgatggc ctcggcaaag ttcggtgcct ctgacagaga tatctctttt
    14941 gccgcctggg tatagaatga ttccggtaaa tcggcgacca gtcgctgcca ggcttgtgcg
    15001 cggcgttctg gtggcgtcat tactgaatcc accccttgca aacggacatt acgcagaata
    15061 aatggcatga ccgtggttgg cagagtaaaa ccacccgcca gaccacaggc agccacgcag
    15121 ccgccgtaat tcatttgcgc cagcactttt gccagcactt tgtcgccaac agtgtcaatt
    15181 gccccagccc agacttgttt ttccagagga cgggattcgg caaactcatc acgagggaga
    15241 atacgactgg cacctaaact tttcagatat tcatgggtac tttcgcgacc ggaaacggca
    15301 acgacctgat aacccaactt atgcagcagc gccacggcgg tactgccgac gccaccactg
    15361 gcacccgtca cgacaatctc cccgtcctgc gggcgaacac cggcatcttc cagcgccatc
    15421 acacacagca tggcggtaaa accggcagta ccgataatca ttgctttacg cgcgtccagc
    15481 ccttgcggca tggcaaccag ccagtcacct ttcactcgcg cctgctccgc cagcccaccc
    15541 cagtggtttt caccaacgcc ccagccagtg agtaacacct cctgaccggc atgaaaacgc
    15601 ggatcttcgc tggtgcgtac agttccggca aaatcgatcc caggaatcat cggaaaatta
    15661 cggatgattt ttcccttacc ggtaatcgcc agcgcatctt tatagttcag gctcgaccag
    15721 tgaacatcaa ccgtgacatc gccctccggc aggcgacttt cgtcaagtgt ctgtaccgat
    15781 gcgagagttt tgccgtcctg ctgttctaaa agtaacgcct gcataagtgg tcctcatgtg
    15841 catgatggat tggaaaataa tttctgaaga ctatactcgc taatggaaaa gcaaagccga
    15901 tgaagcgcaa taaattgcga gataaatctg atttgctagt atgcccgctt cctcactatc
    15961 ggagttaaca caaggatgag attaacgacg aaattttcgg cctttgttac gctgctcacc
    16021 gggttaacaa tttttgtgac tttgctgggc tgttcgctaa gtttctacaa cgcaattcag
    16081 tataagttta gtcatcgcgt tcaggcggtg gcgacggcga ttgataccca ccttgtgtcg
    16141 aatgacttca gcacattaag gccacaaatt accgaattaa tgatgtcggc agatatcgtt
    16201 cgtgtagacc tgctccatgg tgataaacag gtttataccc tggccagaaa tggtagttat
    16261 cgtccggttg gctccagcga tctgtttcgc gaactgagtg ttccgttgat aaagcatccg
    16321 gggatgtcgc tgcgtctggt ttatcaggat ccgatgggca actatttcca ttctttaatg
    16381 actaccgcgc cgctcacggg ggcgattggc tttatcattg ttatgctctt cctggcggta
    16441 cgctggttac aacggcaact tgccgggcaa gaattgctgg aaacccgggc tactcgtatc
    16501 ttaaacggtg agcgtggctc taatgtgttg ggaaccatct atgaatggcc gcccagaacc
    16561 agcagtgcgc tagatacgct gcttcgtgaa attcagaacg cacgcgaaca acacagccgt
    16621 cttgatacgc tgatccgctc ttatgccgcc caggacgtga aaaccggcct caataaccga
    16681 ctctttttcg ataatcagtt agcaacgtta ctggaagatc aggagaaagt aggtacccac
    16741 gggatcgtga tgatgattcg tctgccggat ttcaatatgt tgagcgatac ttgggggcac
    16801 agccaggttg aagaacagtt cttcactctg acgaatctgc tgtcgacatt tatgatgcgc
    16861 taccctggcg cactgctggc gcgttaccac cgcagtgatt ttgctgcgct gttaccgcac
    16921 cggacgttaa aagaggcaga gagcatcgcc ggtcagttaa tcaaagccgt cgataccttg
    16981 ccgaacaata aaatgctcga tcgcgacgat atgatccaca ttggtatctg cgcctggcgt
    17041 agtggtcagg ataccgagca ggtaatggaa catgcagagt ctgccacgcg taatgcggga
    17101 ttgcagggcg gcaatagctg ggctatttac gatgactcgt tgcctgaaaa aggacgcggt
    17161 aatgttcgct ggcgtacgct tatcgagcaa atgctcagtc gcggcggccc gcgcctttat
    17221 caaaaaccgg cggttactcg cgaaggtcag gttcatcatc gcgaactcat gtgccgcatc
    17281 ttcgatggta atgaagaggt tagctcggcg gagtatatgc cgatggtctt gcagtttggc
    17341 ttatcggaag agtatgaccg tctgcaaatc agccgtctta ttccactatt gcgttactgg
    17401 ccagaggaaa atctggcgat tcaggttacc gttgagtcgc tgattcgccc gcgttttcag
    17461 cgttggctgc gcgatacgtt aatgcaatgt gaaaaatcac aacgaaaacg cataattatt
    17521 gaacttgcag aggccgatgt aggtcaacat atcagtcgtt tacaacctgt tattcgttta
    17581 gtgaatgctt taggggtacg ggtagccgtc aaccaggctg gtttgacgct ggtaagcacc
    17641 agttggatca aagaacttaa tgttgagtta ctcaagctcc atccggggct ggtcagaaac
    17701 attgagaagc gaacggagaa ccagctgctg gttcaaagcc tggtggaagc ctgctccggg
    17761 accagcaccc aagtttacgc caccggtgtg cgttcgcgaa gcgagtggca gaccctgatt
    17821 cagcgcggtg ttacaggcgg gcaaggggat tttttcgcgt cctcacagcc acttgatact
    17881 aacgtgaaaa aatattcaca aagatactcg gtttaacctg ccgtttaatc tgttttcacg
    17941 tagaataacg cgcgctgcgt ctcatgggag tgtgcttgtc tgctcgccag attgttgcag
    18001 cacatatgca gatgaatgac cttacgcggt tgcaaacagg cgaggaatgc tgctgatgca
    18061 ttaagccttt ctggactcag gcagagattt gtaacaaagg aaacgaactg cactaatttt
    18121 caccgtagca gatgattttt gcgccttgtc gctgctgcgt gtggttggta aagtaagcgg
    18181 attttctttt ccgccccagc tttcaggatt atcccttagt atgttgaaaa aatttcgtgg
    18241 catgttttcc aatgacttgt ccattgacct gggtactgcg aataccctca tttatgtaaa
    18301 aggacaaggc atcgtattga atgagccttc cgtggtggcc attcgtcagg atcgtgccgg
    18361 ttcaccgaaa agcgtagctg cagtaggtca tgacgcgaag cagatgctgg gccgtacgcc
    18421 gggcaatatt gctgccattc gcccaatgaa agacggcgtt atcgccgact tcttcgtgac
    18481 tgaaaaaatg ctccagcact tcatcaaaca agtgcacagc aacagcttta tgcgtccaag
    18541 cccgcgcgtt ctggtttgtg taccggttgg cgcgacccag gttgaacgcc gcgcaattcg
    18601 tgaatccgcg cagggcgctg gtgcccgtga agtcttcctg attgaagaac cgatggctgc
    18661 cgcaattggt gctggcctgc cggtttctga agcgaccggt tctatggtgg ttgatatcgg
    18721 tggtggtacc actgaagttg ctgttatctc cttgaacggc gtggtttact cctcttccgt
    18781 gcgcattggt ggtgatcgtt tcgacgaagc tatcatcaac tatgtgcgtc gtaattacgg
    18841 ttctctgatc ggtgaagcca ccgcagaacg tatcaagcac gaaatcggtt cggcttaccc
    18901 gggcgatgaa gtccgtgaaa tcgaagttcg tggccgtaac ctggcagaag gtgttccacg
    18961 cggttttacc ctgaactcca acgaaatcct cgaagcactg caggaaccgc tgaccggtat
    19021 tgtgagcgcg gtaatggttg cactggaaca gtgcccgccg gaactggctt ccgacatctc
    19081 cgagcgcggc atggtgctca ccggtggtgg cgcactgctg cgtaaccttg accgtttgtt
    19141 aatggaagaa accggcattc cagtcgttgt tgctgaagac ccgctgacct gtgtggcgcg
    19201 cggtggcggc aaagcgctgg aaatgatcga catgcacggc ggcgacctgt tcagcgaaga
    19261 gtaatcggat gcaggcaggg gaaatgtctg tttaccctgc ctggtctgat acgagaatac
    19321 gcataactta tgaagccaat ttttagccgt ggcccgtcgc tacagattcg ccttattctg
    19381 gcggtgctgg tggcgctcgg cattattatt gccgacagcc gcctggggac gttcagtcaa
    19441 atccgtactt atatggatac cgccgtcagt cctttctact ttgtttccaa tgctcctcgt
    19501 gaattgctgg atggcgtatc gcagacgctg gcctcgcgtg accaactaga acttgaaaac
    19561 cgggcgttac gtcaggaact gttgctgaaa aacagtgaac tgctgatgct tggacaatac
    19621 aaacaggaga acgcgcgtct gcgcgagctg ctgggttccc cgctgcgtca ggatgagcag
    19681 aaaatggtga ctcaggttat ctccacggtt aacgatcctt atagcgatca agttgttatc
    19741 gataaaggta gcgttaatgg cgtttatgaa ggccagccgg tcatcagcga caaaggtgtt
    19801 gttggtcagg tggtggccgt cgctaaactg accagtcgcg tgctgctgat ttgtgatgcg
    19861 acccacgcgc tgccaatcca ggtgctgcgc aacgatatcc gcgtaattgc agccggtaac
    19921 ggttgtacgg atgatttgca gcttgagcat ctgccagcga atacggatat tcgtgttggt
    19981 gatgtgctgg tgacctccgg tctgggcggt cgtttcccgg aaggctatcc ggtcgcggtt
    20041 gtctcttccg taaaactcga tacccagcgc gcttatactg tgattcaggc gcgtccgact
    20101 gcaggactgc aacgtttgcg ttatctgctg ctgctgtggg gcgcggatcg taacggcgct
    20161 aacccgatga cgccggaaga ggtgcatcgt gttgctaatg aacgtctgat gcagatgatg
    20221 ccgcaggtat tgccttcgcc agacgcgatg gggccaaagt tacctgaacc ggcaacgggg
    20281 atcgctcagc cgactccgca gcaaccgacg acaggaaatg cagctactgc gcctgctgcg
    20341 cccacacagc ctgctgctaa tcgctctcca caaagggcta cgccgccgca aagtggtgct
    20401 caaccgcctg cgcgtgcgcc gggagggcaa tagtggcgag ctatcgtagc cagggacgtt
    20461 gggtaatctg gctctctttc ctcattgcgc tgttgctgca aatcatgccc tggccggata
    20521 acctgattgt tttccggcca aactgggtgt tactcatctt gttgtattgg atcctggcct
    20581 tgcctcatcg cgtaaatgtg ggcacaggtt ttgtgatggg tgccatactg gatctgatca
    20641 gcggctcgac gcttggcgta cgcgtattgg cgatgagcat cattgcttac ctggtggcgc
    20701 tgaaatacca gcttttccgc aacctcgcat tatggcagca ggcgctggtc gtcatgttgc
    20761 tttcgctggt ggtggatatt attgttttct gggcagagtt tttagtgatt aacgtctctt
    20821 tcagaccgga agtgttctgg agtagtgtag tcaatggggt gctctggccg tggattttct
    20881 tgctgatgcg taaagtccgt cagcagtttg cagtgcaata aaggtttcta tgacttctct
    20941 gtatttagct tccggttctc cgcgtcgtca ggagttactt gcgcaacttg gcgtgacctt
    21001 tgaacgtatt gttacgggca ttgaggagca gcgtcagccg caggagagcg cgcagcagta
    21061 tgttgtgcgt ctggcgcgcg agaaagcaca ggcgggtgtc gcgcaaacgg cgcaggatct
    21121 cccggtgctg ggtgcggata ctatcgttat cctgaatgga gaagtgctgg agaaaccgcg
    21181 cgacgcagag catgcggcgc agatgttgcg caaattatcg ggtcagaccc atcaggtgat
    21241 gacggcagtg gcgttggccg acagccagca cattctcgat tgcctggtgg tcaccgatgt
    21301 gactttcaga acgttaacag acgaagacat cgcgggctat gtcgccagcg gtgaaccgtt
    21361 agataaagca ggtgcatacg gtattcaggg gctgggtggc tgttttgtca ggaagataaa
    21421 tggcagctat cacgccgtag tcggcttacc gctggttgaa acgtatgaat tattaagtaa
    21481 ttttaacgca ctgcgtgaga aaagggataa acatgacggc tgaattgtta gtaaacgtaa
    21541 cgccttcgga aacgcgagtg gcgtatattg atggcggtat tctgcaggaa attcatattg
    21601 aacgtgaggc gcgacgcgga atagtaggca atatctacaa gggtcgtgta agtcgtgtac
    21661 ttccgggtat gcaggcggct tttgtagata ttgggctgga taaagccgcg tttcttcatg
    21721 catccgatat catgccgcac accgaatgtg tggcgggtga agaacaaaag caattcacgg
    21781 tgcgcgacat ctcggaactg gttcgtcagg ggcaagatct gatggtgcag gtggtgaaag
    21841 atccgcttgg cactaaaggt gcgcgcctga ccaccgatat cacgctgcct tctcgctatc
    21901 tggtgtttat gccaggggct tctcacgttg gggtttccca acgtattgaa agcgaatcag
    21961 aacgtgaacg cctgaaaaaa gtggtcgcag agtattgcga cgagcagggc gggtttatca
    22021 tccgtaccgc agcggaaggg gttggcgagg ctgaactggc ctccgatgcc gcttatctga
    22081 aacgcgtctg gaccaaagtt atggagcgta aaaaacgccc gcagacccgt tatcagctgt
    22141 acggcgaact ggcgctggcg cagcgtgttc tgcgtgattt cgccgacgcc gaactggacc
    22201 gcattcgcgt tgactcacgc ctgacttacg aagcattgct ggagtttacc tcggagtaca
    22261 ttcccgagat gaccagcaag ctggagcatt acaccggacg ccagccgatt ttcgatctct
    22321 ttgatgtcga aaacgaaatc cagcgagcgc tggaacgcaa agtagaactg aaatccggtg
    22381 gttatctgat tatcgaccag accgaagcga tgaccaccgt ggacatcaat accggagcgt
    22441 ttgtcggtca tcgcaatctg gacgacacca ttttcaatac caatattgaa gcgacgcagg
    22501 ctatcgctcg ccagttacgg ttgcgtaatc tgggtgggat tatcattatt gatttcatcg
    22561 atatgaataa tgaagatcac cgccgccgcg tgctgcactc gctggagcag gcgttgagca
    22621 aagaccgggt gaaaaccagc gttaatggtt tttcggcgct ggggctggtg gagatgacgc
    22681 gtaaacgcac ccgcgaaagc attgagcacg tactgtgtaa cgaatgccca acctgccacg
    22741 gtcgcggaac ggtgaaaacc gtggaaacgg tatgctatga aatcatgcgc gagattgttc
    22801 gtgtccacca tgcttacgac tccgaccgtt tcctggtcta tgcatctccg gcagtagctg
    22861 aagccttgaa aggcgaagag tcacactcgc tggcggaagt ggaaattttc gttggcaaac
    22921 aggttaaagt acaaattgaa ccgctctata accaggagca gtttgacgtc gtaatgatgt
    22981 aaacagatgc tgggccgcca tccggcaaag ggtttttgag tcacattttt agcagacaag
    23041 gagtgacggg tgaggcgatt gccggggatt ttactgctta ctggagccgc gctcgttgtg
    23101 atcgctgctc tgctggttag cggcctgcgt attgctttac cgcatcttga cgcctggcgt
    23161 ccggaaatcc tcaacaaaat agaatccgcg actggcatgc cggtagaagc cagtcagctc
    23221 tcagccagct ggcagaattt tggcccgacg cttgaagcac acgacatccg tgcagaacta
    23281 aaagatggcg gcgaattttc ggttaaacgc gttactctgg cgctggatgt ctggcagagc
    23341 ctgttacata tgcgctggca gtttcgcgac ctcactttct ggcagctgcg ctttcgcacc
    23401 aacactccta tcaccagcgg tggtagcgat gacagtctgg aagccagtca catcagcgat
    23461 ctgtttcttc gtcaatttga ccatttcgat ctccgcgaca gtgaagtcag ttttctgacg
    23521 ccatccggtc agcgcgccga gctggcgatc ccacaactca cctggctgaa cgatccacgt
    23581 cgacaccgtg cggaaggcct ggtaagcctc tccagcctta ccggacagca cggcgtgatg
    23641 caggtgcgca tggatttgcg cgatgatgag gggttgttaa gcaatggtcg cgtctggcta
    23701 caggcggatg acatcgacct gaagccgtgg ctcggtaaat ggatgcagga caatattgct
    23761 ctggaaacgg cacagttctc ccttgaaggc tggatgacga tcgacaaagg cgatgtaacc
    23821 ggcggtgacg tctggctgaa acagggcggt gccagctggt tgggcgagaa gcaaacgcat
    23881 acgctgtcgg tggataatct gaccgcgcat attacgcgtg aaaatccggg ctggcagttc
    23941 tctattcccg atacacggat cacgatggac ggcaaaccct ggccgagcgg agcattgacg
    24001 ctggcctgga taccggaaca ggacgttggc ggcaaagaca ataaacgcag tgacgaactc
    24061 cggattcgcg ccagtaatct ggagctggca ggcctggagg gcatacgccc gctggccgcg
    24121 aaactttcac ctgcactggg tgatgtttgg cgctccacac aaccgagcgg caagattaac
    24181 actctggcgc tggatatccc gcttcaggcg gcagacaaga cccgttttca ggcatcgtgg
    24241 agcgatctgg cctggaagca atggaaatta ttaccgggtg cggaacactt ctccgggacg
    24301 ctttccggca gcgttgaaaa tggtttgctt accgcgtcga tgaagcaggc aaagatgcct
    24361 tacgaaacgg tattccgtgc gccactggaa atcgccgacg gccaggcaac tataagctgg
    24421 ctgaacaatg acaaaggttt ccagctggat gggcgtaata ttgacgttaa agccaaagcc
    24481 gtccatgcgc gcggcggttt tcgttacctg caacctgcta acgatgaacc ctggctgggt
    24541 attctggctg gcatcagtac cgatgatggt tcacaagcct ggcgctattt cccggaaaac
    24601 ttgatgggta aagacctggt tgattactta agtggcgcga ttcagggcgg tgaagcggat
    24661 aacgcgacgc tggtttatgg tggcaatccg caactcttcc cctataaaca caacgaaggt
    24721 cagtttgaag tgctggtgcc gctgcgcaac gcgaagtttg ccttccagcc ggactggcct
    24781 gcattaacta accttgatat tgaactggac tttattaacg acggtttatg gatgaaaacc
    24841 gatggcgtta atctgggcgg cgtgcgcgcg agtaatctca ccgcagtgat ccctgactac
    24901 tcgaaagaaa aactgctgat tgacgctgac attaaaggtc cgggtaaagc cgttggccct
    24961 tactttgatg agacaccgct gaaagattct ctgggtgcga ccctgcaaga actccagctc
    25021 gacggcgatg tgaatgctcg cttacatctt gatatcccgc tgaacggcga actggtaacc
    25081 gcgaaaggtg aagtgacgct gcgtaataac agtctgttta tcaaaccact cgacagcacc
    25141 ctgaaaaatt tgagcggtaa attcagcttt atcaatggcg atctgcaaag tgaaccactg
    25201 acagcaagct ggtttaatca gccgttgaac gtggattttt ccaccaaaga aggggcaaaa
    25261 gcctaccagg ttgcggtgaa tctcaacggt aactggcaac cggcgaaaac cagcgttctg
    25321 cctgaagcgg tgaacgaagc attgagtggc agcgtggcgt gggatggtaa agtgggcatt
    25381 gatctgcctt atcatgctgg cgcgacctat aacgtagagc tgaacggcga tctgaataat
    25441 gtgagtagtc acttaccttc accgttagcc aaacctgcgg gtgaaccact agcggtaaac
    25501 gttaaggttg atggcaatct caacagcttt gaattaaccg gacaggctgg tgcggataac
    25561 catttcaata gccgctggtt gcttggtcaa aagctgacgc tcgatcgtgc tatttgggcg
    25621 gcagacagta aaacgctccc gccgttgccg gaacaaagtg gcgttgaact caatatgccg
    25681 ccgatgaatg gtgccgagtg gctggccctg ttccagaaag gcgctgcgga gagtgtcggt
    25741 ggtgcagcga gtttcccaca acacataacg ttacgtacgc ctatgttgtc actgggaaat
    25801 cagcaatgga ataacctgag tattgtttcg caaccgacgg caaatggcac cctggttgag
    25861 gcgcaagggc gtgaaatcaa cgccacgcta gcgatgcgta ataacgcgcc gtggctggcg
    25921 aatatcaaat atctttatta caacccgagc gtggcgaaaa ctcgtggtga ttcaacgccg
    25981 tcatcacctt tcccgacaac ggagcgcatt aacttccgtg gctggccgga cgcacaaata
    26041 cgatgcacag agtgctggtt ctgggggcaa aaattcggtc gcattgacag tgatatcacc
    26101 atttctggcg atacgttaac gctgaccaat ggactgattg atactggttt ctcgcggctt
    26161 actgccgatg gtgaatgggt taataatccg gagaatgaac gtacctcgct gaaaggaaaa
    26221 ctgcgcgggc agaaaattga tgccgccgca gaattttttg gtgtcacgac gcccatacgc
    26281 cagtcgtcat ttaatgtgga ttacgattta cactggcgca aagcaccctg gcaaccagat
    26341 gaggcgacgt tgaatggcat cattcatact caactgggta aaggcgaaat taccgaaatc
    26401 aataccggac atgccgggca attgctgcgc ttattgagcg ttgatgccct gatgcgtaag
    26461 ctgcgttttg atttcagaga cacttttggc gaagggttct attttgactc cattcgcagc
    26521 accgcgtgga ttaaagacgg ggttatgcac accgacgaca cgctggtgga tggcctggag
    26581 gcggatatcg ccatgaaagg gtcggtaaat ctggtacgtc gcgacctgaa tatggaagcg
    26641 gttgtcgcac cagagatttc tgcgacggtg ggcgtggctg cggcttttgc ggttaacccc
    26701 attgttggcg cggcagtatt tgcagccagt aaagtgctgg ggccgctgtg gagcaaagtc
    26761 tccattttgc gctatcacat ttcgggtccg ctggacgatc cgcaaatcaa cgaagtgttg
    26821 cgccaaccgc gtaaagaaaa agcgcaatga tttgacgagg gcgcgtaatt gccccaatct
    26881 catatgataa tcgttgccaa aggccaacga gccggaacat aactgtaggc cggatagggc
    26941 gttcacgccg catccggcag ccgtaaaaaa tcctctactg cagtaactaa cgagtagcaa
    27001 aaacgatgag tctgaacctg gtaagtgaac aattgctagc ggcgaacggc ctgaaacatc
    27061 aggacttgtt cgcgatcctc ggtcaactgg ccgaacgtcg ccttgattat ggcgatctct
    27121 attttcagtc gagctatcac gaatcctggg ttttagaaga ccgcattatt aaagatggtt
    27181 cttacaacat cgatcagggc gttggtgtgc gtgcaatcag tggtgaaaaa accggatttg
    27241 cttacgctga ccaaatcagc ctgctggcgc tggaacagag tgcgcaagcg gcgcgcacca
    27301 tcgtccgtga tagtggtgat ggcaaagtac agacgctggg cgcggtagag catagcccgt
    27361 tgtatacctc ggtagatccg ctgcaaagca tgagccgtga agagaagctg gatatcctgc
    27421 gtcgcgtcga taaggttgcc cgcgaagcgg acaagcgcgt acaagaagtg actgccagcc
    27481 tcagcggcgt ttatgaatta attctggttg cggccaccga cggcacgctg gcggcggatg
    27541 ttcgtccgct ggtgcgtctt tccgtgagcg ttctggtcga agaagatggc aaacgcgaac
    27601 gcggtgccag tggcggcggc ggtcgttttg gttatgagtt cttccttgcc gatcttgacg
    27661 gcgaagtccg tgcggatgca tgggcaaaag aagcagtgcg tatggcgctg gtcaatcttt
    27721 ctgccgttgc tgcaccagcg ggcaccatgc cggtagtact tggcgcaggt tggccgggcg
    27781 tgctgttgca tgaagcggta ggtcacggtc tggaaggcga cttcaactgc cgcggcactt
    27841 cagtatttag tgggcaggtc ggggagctgg tggcttcaga actgtgtacc gtggttgatg
    27901 acggcacgat ggtcgatcgc cgtggttcgg tggcgattga tgacgaaggt acgccaggcc
    27961 agtacaacgt gctgattgag aacggcattc tgaaaggcta catgcaggat aaactcaacg
    28021 cgcgtttgat ggggatgacg ccgactggca acggtcgccg tgaatcctac gcccatctgc
    28081 ccatgccgcg tatgaccaac acctatatgc tgccgggtaa atcgaccccg caggaaatta
    28141 ttgaatccgt tgagtacggt atctatgcac cgaactttgg tggcggtcag gtggatatta
    28201 catccggcaa attcgttttc tccacttcag aagcgtatct gattgaaaac ggtaaagtaa
    28261 cgaagccggt gaaaggcgcg acgttgattg gttccggtat cgaaaccatg cagcagattt
    28321 cgatggttgg caacgacctg aaactggata acggcgtggg tgtctgcggt aaagaagggc
    28381 aaagtttgcc ggttggcgtg ggccagccaa cgctgaaagt tgataacctg actgttggcg
    28441 gtactgcgta ataatttcat tattttcaga aggataatta atctttctac gtgcacgaac
    28501 ggtcccctcg ctcctctggg gttagggtga ggggaacccg ttggcacagg tttgtagaac
    28561 gtaacagtac aatatgaatt acttctcttt cccgcgcccg tgcatctcct gaaacaattt
    28621 accgacctca acaaaataat ccgtcagcga gttgatcacg acctgtacct tcagcggcag
    28681 cttatctttt tcggtatata acgcataaac cgggcgtgga tctgactgat aacgcggcag
    28741 caggatctcc agctcaccgc gattgatctc gttgatcacc cacatcagcg gcacgtaggc
    28801 gatcccggca cccgccgtta gccagcgcac cagcgtcatc ggatcattag tcacaaatct
    28861 cccttgcggg atcaggcgag tcgagatccc ttccggtgca atcagttcaa attcattgtc
    28921 gggccgcacg ctgtattcaa gccatgaatg gctacttaaa tcggcgggtt tttccggtat
    28981 gccgtattgt gtgagatagc ttttcgcggc gcacaccacc attggcatcg cgccaagacg
    29041 gcgggaaaac aggctggaat cctgcaacgc gccgacgcgg atcaccacat ccagaccgtc
    29101 ggcaatcagg tcgggggctg gaattccggt aaccagattg acgctcaaac ctgggtattc
    29161 tttcagcatt ttggctgtca gcccggcgag aacattttgt gccatagttg aagaacagcc
    29221 aatgcgtagc gtcccgatgg gggtgttatt gaaggcatac agttgctcat gaacatcctg
    29281 cacttcatga agcatacgac ggcagccctg gtagtaaatt ctaccggctt cggtcaggcc
    29341 aatgctgcgt gtgctacggt ttaacagctt tacctgcaac tcatcttcca gttttgacac
    29401 cgtctgactg atggacgaaa cgctcatctg tagctgtctg gcggcggcgg taaaagagcc
    29461 aaattcaact actttggcaa acaccgacat gcgttttagt cgttccatta ttcactctga
    29521 cttaaaagtg atttagatca cataatatag ataacagcat aacagttacg ctaatatatt
    29581 aaatatcaat ctacagcaat gttgctctcg cccggctttc catgcccttc tctgcggcga
    29641 cagatgctga aaataataac gcctgctctc tctatacaac caaggtcaac atgagtctgt
    29701 ttcccgttat cgtggtgttt gggctgtcct tcccaccgat attttttgaa ttgcttttat
    29761 cactggcgat tttctggctg gtgcgccggg tacttgtgcc aacaggtatc tacgactttg
    29821 tctggcatcc ggcgttgttc aacaccgcgc tctattgctg cttgttttat ttgatatcgc
    29881 gactgttcgt ttgaggttga agtgaaaaca ctaataagaa aattctcccg tacggccatc
    29941 acggtcgtat tagtcattct ggccttcatc gcaattttta atgcctgggt ctattacacc
    30001 gaatccccct ggacgcgtga cgcgcgcttt agcgctgacg tcgttgcgat cgcgccggac
    30061 gtttctggac tcattaccca ggtgaatgtt catgataacc agctggtgaa aaaaggacag
    30121 atactgttca ccatcgacca gccgcgctat caaaaggcgc ttgaggaagc gcaagccgat
    30181 gttgcctatt atcaggtact ggcacaggag aaacgccagg aggccggacg tcgtaaccgt
    30241 ctcggtgtgc aggcgatgtc tcgcgaagag atcgaccagg ctaacaacgt actacaaacg
    30301 gttctgcatc agttagcgaa agcgcaggcg acccgcgatc tggcaaaact ggatcttgaa
    30361 cgcacggtga tccgcgcgcc agcagatggc tgggtgacca acctcaacgt ctataccggt
    30421 gagtttatta ctcgaggatc aacggcggtt gcgctggtga aacagaactc cttctatgta
    30481 ctggcctata tggaagaaac taagctggaa ggggtgcgtc cggggtatcg tgcagagatc
    30541 acgccgcttg gcagtaacaa agtgctgaaa gggactgttg atagtgttgc cgctggggtc
    30601 accaacgcca gcagcacgcg tgacgacaaa gggatggcga ccatcgactc taaccttgaa
    30661 tgggtgcgtt tggcgcaacg tgttccggtt cgtattcgtc tcgacaacca gcaagagaac
    30721 atctggcctg cgggcaccac tgctacagtg gtggtcactg gcaaacaaga tcgcgacgaa
    30781 agccaggatt cgttcttccg taaaatggcc catcgcctgc gtgagtttgg ttaatcacga
    30841 tgggtatttt ctccattgct aaccaacata ttcgctttgc ggtaaaactg gcgaccgcca
    30901 ttgtactggc gctgtttgtt ggctttcact tccagctgga aacgccacgc tgggcggtac
    30961 tgacagcggc gattgttgcc gccggtccgg cctttgctgc gggaggtgaa ccgtattctg
    31021 gcgctattcg ctatcgtggc tttttgcgca tcatcggcac atttattggc tgtattgccg
    31081 gactggtgat catcattgcg atgatccgcg caccattatt gatgattctg gtgtgctgta
    31141 tctgggccgg tttttgtacc tggatatcct cgctggtacg aatagaaaac tcgtacgcgt
    31201 gggggctggc cggttatacc gcgctgatca ttgtgatcac cattcagccg gaaccattgc
    31261 ttacgccgca gtttgccgtc gaacgttgta gcgagatcgt tatcggtatt gtgtgtgcga
    31321 ttatggcgga tttgctcttt tctccgcgat cgatcaaaca agaagtggat cgagagctgg
    31381 aaagtttgct ggtcgcgcaa tatcaattaa tgcaactctg tatcaagcat ggcgatggtg
    31441 aagttgtcga taaagcctgg ggcgacctgg tgcgacgcac cacggcgcta caaagcatgc
    31501 gcagcaacct gaatatggaa tcttcccgct gggcgcgggc caatcgacgt ttaaaagcga
    31561 tcaatacgct atcgctgacg ctgattaccc aatcctgcga aacttatctt attcagaata
    31621 cgcgcccgga attgatcact gatactttcc gcgaattttt tgacacgccg gtagaaaccg
    31681 cgcaggacgt ccacaagcag ctcaaacgcc tgcggagagt tatcgcctgg accggggaac
    31741 gggaaacgcc tgtcaccatt tatagctggg tcgcggcggc aacgcgttat cagcttctca
    31801 agcgcggcgt tatcagtaac acaaaaatca acgccaccga agaagagatc ctgcaaggcg
    31861 aaccggaagt caaagtagag tcagccgaac gtcatcatgc gatggttaac ttctggcgaa
    31921 ccacactttc ctgcattctg ggcacgcttt tctggctgtg gacgggctgg acttccggca
    31981 gtggtgcaat ggtgatgatt gcggtcgtga cgtcactggc aatgcgtttg ccgaatccac
    32041 gcatggtggc gatcgacttt atctacggga cgctggccgc gctgccgtta gggctgctct
    32101 actttttggt gattatccct aatacccaac agagcatgtt gctgctgtgt attagcctgg
    32161 cagtgctggg atttttcctc ggtatagaag tacagaaacg gcgactgggc tcgatggggg
    32221 cgctggccag caccataaat attatcgtgc tggataaccc gatgactttc catttcagtc
    32281 agtttctcga cagcgcatta gggcaaatcg tcggctgtgt gctcgcgttc accgttattt
    32341 tgcttgtgcg ggataaatcg cgcgacagga ccggacgtgt actgcttaat cagtttgttt
    32401 ctgccgctgt ttccgcgatg actaccaatg tggcacgtcg taaagagaac cacctcccgg
    32461 cactttatca gcagctgttt ttgctgatga ataagttccc aggggatttg ccgaaatttc
    32521 gcctggcgct gacgatgatt atcgcgcacc agcgcctgcg tgatgcgccg atcccggtta
    32581 acgaggattt atcggcgttt caccgacaaa tgcgccgcac agcagaccat gtgatatctg
    32641 cccgtagcga tgataaacgt cgtcggtact ttggccagtt gctggaagaa ctggaaatct
    32701 accaggaaaa gctacgcatc tggcaagcgc caccgcaggt gacggaaccg gttcatcggc
    32761 tggcggggat gctccataag tatcaacatg cgttgaccga tagttaagtc aaaaccgacg
    32821 ccaaaagcgt cggttttttc atggctatac ttagcgatgc acggcagaac tcgccgcgaa
    32881 acgtgacggt ggcaacagat gaatatttat acctttgatt ttgatgagat tgagagtcag
    32941 gaggattttt atcgtgactt tagccaaacc tttggtctgg ctaaagataa ggtacgcgat
    33001 ctcgactcac tatgggatgt gttaatgaac gatgtcctgc cgctaccact tgagattgaa
    33061 tttgttcatc tgggagagaa aacgcgtcgc cgttttggcg cgttaatatt gctgtttgat
    33121 gaggcagagg aagagctgga agggcatttg cgttttaatg ttcgtcatta gcgcaaaaaa
    33181 aagcccccga accgggggca atatcgtcgg acaagacgat gagggtttat ttgtacagtt
    33241 cagccgtagc gtgccaggtg tcaccgctac gagcttcagt aatctggtag gccgttgcgc
    33301 ctttctcttc cgcttttttg ttcagcattt cacgcatatc cattggcgaa gacgccacac
    33361 cacttacgga tacggtcccg attgcttcac gattttgtgc ttgtgcagca tcaatggagt
    33421 cggcagcgaa tgcaccgaaa gagagaacag aaagtacgct taatgcagct acagtggttt
    33481 tgattttcat gatttttacc tcgacataat cttttagctg ggcctttgtt tcgtgaccca
    33541 tatcacaaaa tcaagtatac actaattatt gaactaatta at
//
LOCUS       DAZGJW010000040        33161 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZGJW010000040 DAZGJW010000000
VERSION     DAZGJW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.040"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..295)
                     /locus_tag="ACHKIK_001927"
     CDS             complement(<1..295)
                     /locus_tag="ACHKIK_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFO9083110.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELA"
     gene            476..628
                     /locus_tag="ACHKIK_001928"
                     /pseudo
     CDS             476..628
                     /locus_tag="ACHKIK_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            1252..1551
                     /locus_tag="ACHKIK_001929"
     CDS             1252..1551
                     /locus_tag="ACHKIK_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083111.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(1598..2485)
                     /gene="rarD"
                     /locus_tag="ACHKIK_001930"
     CDS             complement(1598..2485)
                     /gene="rarD"
                     /locus_tag="ACHKIK_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO9083112.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(2537..3004)
                     /gene="yigI"
                     /locus_tag="ACHKIK_001931"
     CDS             complement(2537..3004)
                     /gene="yigI"
                     /locus_tag="ACHKIK_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO9083113.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3169..4038
                     /gene="pldA"
                     /locus_tag="ACHKIK_001932"
     CDS             3169..4038
                     /gene="pldA"
                     /locus_tag="ACHKIK_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO9083114.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            4171..6000
                     /gene="recQ"
                     /locus_tag="ACHKIK_001933"
     CDS             4171..6000
                     /gene="recQ"
                     /locus_tag="ACHKIK_001933"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO9083115.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            6064..6684
                     /gene="rhtC"
                     /locus_tag="ACHKIK_001934"
     CDS             6064..6684
                     /gene="rhtC"
                     /locus_tag="ACHKIK_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO9083116.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(6746..7366)
                     /gene="rhtB"
                     /locus_tag="ACHKIK_001935"
     CDS             complement(6746..7366)
                     /gene="rhtB"
                     /locus_tag="ACHKIK_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO9083117.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7477..8499
                     /gene="pldB"
                     /locus_tag="ACHKIK_001936"
     CDS             7477..8499
                     /gene="pldB"
                     /locus_tag="ACHKIK_001936"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO9083118.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8507..9307
                     /gene="yigL"
                     /locus_tag="ACHKIK_001937"
     CDS             8507..9307
                     /gene="yigL"
                     /locus_tag="ACHKIK_001937"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO9083119.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            9383..10282
                     /gene="bioP"
                     /locus_tag="ACHKIK_001938"
     CDS             9383..10282
                     /gene="bioP"
                     /locus_tag="ACHKIK_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO9083120.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(10170..11123)
                     /gene="metR"
                     /locus_tag="ACHKIK_001939"
     CDS             complement(10170..11123)
                     /gene="metR"
                     /locus_tag="ACHKIK_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO9083121.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            11241..13502
                     /gene="metE"
                     /locus_tag="ACHKIK_001940"
     CDS             11241..13502
                     /gene="metE"
                     /locus_tag="ACHKIK_001940"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO9083122.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRLG"
     gene            complement(13541..14356)
                     /gene="ysgA"
                     /locus_tag="ACHKIK_001941"
     CDS             complement(13541..14356)
                     /gene="ysgA"
                     /locus_tag="ACHKIK_001941"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO9083123.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            14618..15379
                     /gene="udp"
                     /locus_tag="ACHKIK_001942"
     CDS             14618..15379
                     /gene="udp"
                     /locus_tag="ACHKIK_001942"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO9083124.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            15520..16947
                     /gene="rmuC"
                     /locus_tag="ACHKIK_001943"
     CDS             15520..16947
                     /gene="rmuC"
                     /locus_tag="ACHKIK_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO9083125.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFSLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            17042..17797
                     /gene="ubiE"
                     /locus_tag="ACHKIK_001944"
     CDS             17042..17797
                     /gene="ubiE"
                     /locus_tag="ACHKIK_001944"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO9083126.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            17811..18416
                     /gene="ubiJ"
                     /locus_tag="ACHKIK_001945"
     CDS             17811..18416
                     /gene="ubiJ"
                     /locus_tag="ACHKIK_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO9083127.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            18413..20053
                     /gene="ubiB"
                     /locus_tag="ACHKIK_001946"
     CDS             18413..20053
                     /gene="ubiB"
                     /locus_tag="ACHKIK_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO9083128.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            20132..20401
                     /gene="tatA"
                     /locus_tag="ACHKIK_001947"
     CDS             20132..20401
                     /gene="tatA"
                     /locus_tag="ACHKIK_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO9083129.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            20405..20920
                     /gene="tatB"
                     /locus_tag="ACHKIK_001948"
     CDS             20405..20920
                     /gene="tatB"
                     /locus_tag="ACHKIK_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO9083130.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            20923..21699
                     /gene="tatC"
                     /locus_tag="ACHKIK_001949"
     CDS             20923..21699
                     /gene="tatC"
                     /locus_tag="ACHKIK_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO9083131.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            21741..22523
                     /gene="tatD"
                     /locus_tag="ACHKIK_001950"
     CDS             21741..22523
                     /gene="tatD"
                     /locus_tag="ACHKIK_001950"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO9083132.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(22520..23008)
                     /gene="rfaH"
                     /locus_tag="ACHKIK_001951"
     CDS             complement(22520..23008)
                     /gene="rfaH"
                     /locus_tag="ACHKIK_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO9083133.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            23175..24668
                     /gene="ubiD"
                     /locus_tag="ACHKIK_001952"
     CDS             23175..24668
                     /gene="ubiD"
                     /locus_tag="ACHKIK_001952"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO9083134.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            24714..25415
                     /gene="fre"
                     /locus_tag="ACHKIK_001953"
     CDS             24714..25415
                     /gene="fre"
                     /locus_tag="ACHKIK_001953"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO9083135.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(25607..26770)
                     /gene="fadA"
                     /locus_tag="ACHKIK_001954"
     CDS             complement(25607..26770)
                     /gene="fadA"
                     /locus_tag="ACHKIK_001954"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO9083136.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(26780..28969)
                     /gene="fadB"
                     /locus_tag="ACHKIK_001955"
     CDS             complement(26780..28969)
                     /gene="fadB"
                     /locus_tag="ACHKIK_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO9083137.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALKRPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            29159..30490
                     /gene="pepQ"
                     /locus_tag="ACHKIK_001956"
     CDS             29159..30490
                     /gene="pepQ"
                     /locus_tag="ACHKIK_001956"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO9083138.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            30490..31104
                     /gene="yigZ"
                     /locus_tag="ACHKIK_001957"
     CDS             30490..31104
                     /gene="yigZ"
                     /locus_tag="ACHKIK_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO9083139.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            31143..32594
                     /gene="trkH"
                     /locus_tag="ACHKIK_001958"
     CDS             31143..32594
                     /gene="trkH"
                     /locus_tag="ACHKIK_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO9083140.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFLVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            32606..33151
                     /gene="hemG"
                     /locus_tag="ACHKIK_001959"
     CDS             32606..33151
                     /gene="hemG"
                     /locus_tag="ACHKIK_001959"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO9083141.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7785 a         8307 c         8978 g         8091 t
ORIGIN      
        1 ttgctagttc tttcatttct gcaaataaaa tatcagtgca ttcacgttgt gatatttttg
       61 ttccggaact tgaggcacta aaagcaacat taatagaacc tgggccaggg aaatggtatt
      121 taataagtct gtaaggatca acttttgcag agatatcgct taggccgagt gagagaaagt
      181 cttgttctac catatcacca tattgcatat catcagcaga aaaatcatcg aataaactaa
      241 tagtttcgaa aagtgtacag ggaatattaa ttggcggaag taatatgtta ttcatttgca
      301 tttgccttgg ttatatttaa gctcaatatc aattcgagaa caaatgctag ggtaaaaaaa
      361 tatttaaaaa agaagtaaac ttcacatttt ttcattacat ctctgaagag aacctcgtta
      421 atgattggtc ttgctcattg ttaataacaa aatgttgaat ttaccgatta ggaaactttt
      481 tcctacgcgg attgcctcca gctatggcat gaactttgag tttatgccag aactgaagtg
      541 gggcttcggc taccctggcg cgattatctt tatgatcctc gcgggcctga caccgtatct
      601 gtactttaag cgtaagaact ggctgtaact atacgatgtc gctgtgagta agttagtgtg
      661 ctgacagcga cattttagcc gagggaatcg cagataacat aattgttgtg aataatatcc
      721 atgccacgtt gagttggacg cttaatgact cacatctcac cccttcaatt gctgcagaga
      781 taatagaaaa aagtgtcaag cccaatgaaa tcaatacaaa gtagattgcc ttacatccag
      841 tatctcttat tctttttgct ccaatattga tatttacata ggtattatta ataaccagaa
      901 taaaactcaa agccaggttg atatcattca aagttattaa tattgaccat gatgttaatg
      961 tatagattat gaactgaaag cgtgataaac gctcaagcct ggatgggtag aatatatttc
     1021 tgatggatga tttgaacatt tttacattgt tttatatata attgttttga atatatatta
     1081 cctgaattat gtggtttgca tatatatatg caatatagca gtctttgcat tgataactat
     1141 acagtagtaa tttagagtgt gtaagttttt attgatgggg tatattttaa ttaaatataa
     1201 ttgaaatgta tttttattct ataaacaata tcttgggtga atggattcta tatgggaaat
     1261 ggtaactttt ggcagttgtt cttgggtaaa gaaccttaca gcaaaaaatc tgattttgtt
     1321 aaggcatggt tattgtggtg ggctattacc atccttgttc tatttatttt aagcctgact
     1381 acgacattaa atttaagaag tataggtata gggttaagtt ttgggttttg tcaaatcctt
     1441 ggtagatata cgatgtctct aggatggagt aagtactggg gagttttggg ggtatttcct
     1501 gtaacaaata ttgttctttt tcttatctta atttttttca aaaaaagata aataagtcag
     1561 tgggtaggct ctttaaacgg cctacccatg ttaattctta actttttcta cgctgcgtat
     1621 aaatcgcatc catcacaaaa attgccagcg ccacccaaat aaaggcgaaa gtcaccatct
     1681 tatcggcacc cggtttttca ccataaaacg tcacagccag caggaacatc agcgtcgggc
     1741 caatgtactg gaaaaagcct aacgttgaga gacgcaagcg cgtggcagcg gcggtaaaac
     1801 acaacagcgg tacggtagtg acaataccgg cggcgatcag cagtaaattc agcgacatcg
     1861 ggttttgccc catatggctg gttgagctgt cggcaatagc aaacaggtaa attgccgcca
     1921 cgggcagcag ccacatggtt tcgattaaca tgccggtttg cgcttcaacg gcaatcttct
     1981 tgcgtaccag accgtagaag gcaaaactaa atgccagtcc cagcgcgata ataggtagcg
     2041 aaccaaaagt ccacagctgg actaacacgc cacatatcgc cagaatcacc gccagccatt
     2101 gcatccggcg gaatcgctcg ccgaggaaaa tcatccccag cacaatgttc accagcgggt
     2161 taataaagta accaaggctc gcttccagca tatggtgatt gttcaccgcc cagataaaca
     2221 gtagccagtt gccaccaatc agcacggcag agactgccag cataaaaatt ttctgtggcg
     2281 tctgaatcag cgtttttaaa taggaccact ggcggcaaat gctcatcagc accaccataa
     2341 agaaaaacga ccagatcacg cgatgcgtca ggatttcatc ggcaggcacg tagtaaatca
     2401 acttgaagta cgctggcgct ataccccaaa taaaataagc ggcaagagcg agtaatacgc
     2461 cctgccgcgt ttgttttgca tccatcggga atactcattt ttaattggta acagcagttt
     2521 acctgctttt atgtcttcaa cctaccatat aggtggcggt ggcactggca atataaagct
     2581 gttcttcatt gtgtaattca acgcgggcga cggcgacttt attgcctgca cgcaacaggc
     2641 tactggtagc agtaaaacgc tcgcccctgc ctgggcgcag ataatcaacg cgaagatcaa
     2701 tggtccccat ccgcgatagc cgctggcgta gttcatcttc actgatggtt tcgtggcggg
     2761 ttaaggtact tcccacgcac accagacctg cggcgacatc cagcgctgac gcaataaccc
     2821 cgccgtgcaa aatgctttgc gcccagttac ccaccatcat tggctgattt ttaaaggcca
     2881 gctgtgcgaa ctctttttcg taacgctcca gctccatccc taacgcgcgg ttaaatggca
     2941 tgtgataaac aaacatctca cccactaatt tcagggcttg ttcagcggtc agtacggcag
     3001 acatatcatc cttacacttc attggttaat gaaatgttga ttttatgctt ctttgttgtt
     3061 ggtttctact ttaggaaggg ataactaacg gctatggagt taagtatgta aaatagcccg
     3121 cagaaaaata ttcaccttat caataattcg ttacggagaa cacgaccgat gcggactctg
     3181 cagggctggt tgttgccggt gtttatgttg cctatggcag tatatgcaca agaggcaacg
     3241 gtgaaagagg tgcatgacgc gccagcggtg cgtggcagta ttatcgccaa tatgctgcag
     3301 gagcatgaca atccgttcac gctctatcct tatgacacca actacctcat ttacacccaa
     3361 accagcgatc tgaataaaga agcgattgcc agttacgact gggcggaaaa tgcgcgtaag
     3421 gatgaagtaa agtttcagtt gagcctggca tttccgctgt ggcgtgggat tttaggcccg
     3481 aactcggtgt tgggtgcgtc ttatacgcaa aaatcctggt ggcaactgtc caatagcgaa
     3541 gagtcttcac cgtttcgtga aaccaactac gaaccgcaat tgttcctcgg ttttgccacc
     3601 gattaccgtt ttgcaggttg gacgctgcgc gatgtggaga tggggtataa ccacgactct
     3661 aacgggcgtt ccgacccgac ctcccgcagc tggaaccgcc tttatactcg cctgatggca
     3721 gaaaacggta actggctggt agaagtgaag ccgtggtatg tggtgggtaa tactgacgat
     3781 aacccggata tcaccaaata tatgggttac taccagctta aaatcggcta tcacctcggt
     3841 gatgcggtgc tcagtgcgaa aggacagtac aactggaaca ccggctacgg cggcgcggag
     3901 ttaggcttaa gttacccgat caccaaacat gtgcgccttt atactcaggt ttacagcggc
     3961 tatggcgaat cgctcatcga ctataacttc aaccagaccc gtgtcggtgt gggggttatg
     4021 ctaaacgatt tgttttgatg aacggttgag tggttggcaa atctggaacc cagcatccag
     4081 gattaccctc tcagagacta aaagcattgc agtttctcgc gcaggcgctg aaaatagcgc
     4141 ctgtttttat ttcaggcaat cggggtgaat gtggcgcagg cggaagtgtt gaatctggag
     4201 tccggagcta aacaggtttt acaagaaacc tttggctacc aacagtttcg ccccggccag
     4261 gaagaaatta tcgacactgt gctttccggt cgcgattgcc tggtcgtcat gcccaccggt
     4321 ggcggaaaat ccctttgcta tcaaattcct gccttattgc taaacggcct taccgtggtt
     4381 gtttcaccgc tgatttcgtt gatgaaagat caggtggatc aactgcaagc caacggcgtg
     4441 gcggcggcgt gccttaactc gacgcaaacc cgcgaacagc aacttgaagt gatgacaggc
     4501 tgccgcaccg ggcaaattcg tctgctttat atcgccccgg aacgcctgat gctggataac
     4561 tttcttgagc atctggcgca ctggaatccg gtgttattag ccgttgatga agcgcactgt
     4621 atctcccaat ggggccacga tttccgcccg gaatatgccg cgctcggtca gttgcgccag
     4681 cggttcccga cgctgccgtt tatggcgctg accgccacag ccgacgacac cacgcgccag
     4741 gatatcgtgc gcctgctggg gctgaacgat ccgctgattc aaatcagcag ttttgaccgt
     4801 ccgaatattc gctacatgct gatggagaag ttcaaaccgc tcgatcagtt gatgcgctac
     4861 gtgcaggaac agcgcggtaa gtccggcatt atctactgca acagccgggc gaaagtagaa
     4921 gacaccgctg cgcgcctgca aagcaagggt attagcgcgg cggcctatca tgccgggctg
     4981 gaaaataatg tccgcgccga cgtgcaggag aaattccagc gcgatgacct gcaaattgtg
     5041 gtggcgacgg tggcgttcgg catgggcatc aataaaccaa acgttcgctt cgtggtccac
     5101 tttgatattc cgcgcaatat cgaatcctat tatcaggaaa ccggtcgcgc cgggcgtgat
     5161 ggcctgcccg cggaagcgat gctgttttac gatccggccg atatggcgtg gctgcgccgt
     5221 tgtctggaag agaagccgca ggggcagttg caggatatcg aacgtcataa actcaatgcg
     5281 atgggcgctt ttgccgaagc gcaaacttgc cgtcgtctgg tactgctgaa ctattttggc
     5341 gaagggcgtc aggagccgtg cgggaactgc gatatctgcc tcgatccgcc gaaacagtac
     5401 gacggttcaa ccgatgctca gattgccctt tccaccattg gtcgtgtgaa tcagcgtttt
     5461 ggtatgggct atgtggtgga agtgattcgc ggcgctaata accagcgtat ccgcgactat
     5521 ggtcatgaca aactaaaagt ctacggtatg ggccgtgata aaagtcatga acattgggtg
     5581 agcgtgatcc gccagctgat tcacctcggt ctggtgacgc aaaatattgc ccagcattct
     5641 gccctacaac tgacagaggc tgcgcgtccg gtgctgcgcg gcgaatcctc tttgcaactt
     5701 gccgtgccgc gtatcgtggc gctcaaaccg aaagcgatgc agaaatcgtt cggcggcaac
     5761 tatgatcgca aactgttcgc caaattacgc aaactgcgta aatcgatagc cgatgaaagt
     5821 aatgtcccgc cgtacgtggt gtttaacgac gcaaccttga ttgagatggc tgaacagatg
     5881 ccgatcaccg ccagcgaaat gctcagcgtt aacggcgttg ggatgcgcaa gctggaacgc
     5941 tttggcaaac cgtttatggc gctgattcgt gcgcatgttg atggcgatga cgaagagtag
     6001 tcagcagcat aaaaaagtgc cagtatgaag actccgtaaa cgtttccccc gcgagtcaaa
     6061 tgtatgttga tgttatttct caccgtcgcc atggtgcaca ttgtggcgct tatgagcccc
     6121 ggtcccgatt tcttttttgt ctctcagacc gctgtcagtc gttcccgtaa agaagcgatg
     6181 atgggcgtgc tgggcattac ctgcggcgta atggtttggg ctgggattgc gctgcttggc
     6241 ctgcatttga ttatcgaaaa aatggcctgg ctgcatacgc tgattatggt gggcggtggc
     6301 ctgtatctct gctggatggg ttaccagatg ctacgcggtg cactgaaaaa agaggcggtt
     6361 tctgcacctg cgccacaggt cgagctggcg aaaagtgggc gcagtttcct gaaaggttta
     6421 ctgaccaatc tcgctaatcc gaaagcgatt atctactttg gctcggtgtt ctcattgttt
     6481 gtcggtgata acgttggcac taccgcgcgc tggggcattt ttgcgctgat cattgtcgaa
     6541 acgctggcgt ggtttaccgt cgttgccagc ctgtttgccc tgccgcaaat gcgccgtggt
     6601 tatcaacgtc tggcgaagtg gattgatggt tttgccgggg cgttatttgc cggatttggc
     6661 attcatttga ttatttcgcg gtgatgccag acgcgtcttc agagtaagtc ggataaggcg
     6721 tttacgccgc atccgacatt atttttcacg catgcctcgc cgatgctaac agcgctccca
     6781 ccagcataaa caacgagccg aaaatcttat tcagcgcctt catctgcttt ggtcctttaa
     6841 tccatagagc aatccgttga gcaagggtgg cgtaaccgat catcacaata atatcgacca
     6901 caatagtggt gacgccgagc acgatatact gcatcagttg cggctgttgc ggcatgatga
     6961 attgcggaaa tagcgccgcc agaaacacaa tacttttggg attggtgaga ttcacaaaaa
     7021 ctgcgcgctg gaacaaatgt cgacgcgatt gagtagaggc cagcgattta aggtcaattg
     7081 caccagcggc gcgccactgc tggattccca gccaaatcaa gtaagccgcg cctgcccact
     7141 tcaacacttc aaacgcaatc actgagcggg aaaatagcgt ccccaacccc acgccaacca
     7201 gcacaatatg aatcgccagt ccggtctgaa gcccagcaat agacgccacc gcgccgcgat
     7261 aaccgtggtt gagcgaggtg gtcatagtgt tgattgcacc agagcctggc gacaggctta
     7321 aaatgatcga tgtcagcagg taggcaaacc accattctaa ggtcatgatg aactcccggt
     7381 gtgtctattt ttgtgccaca atacgctact gtcgcagcgt tgtgtcaggc acgctaaaaa
     7441 aaacgatttt acgtggttta agaggcagat tacccgatgt ttcagcagca aaaagactgg
     7501 gaaacaagag aaaacgcgtt tgctgctttt accatgggac cgctgactga tttctggcgt
     7561 cagcgtgatg aagcagagtt tactggtgtg gatgacattc cggtgcgctt tgtccgtttt
     7621 cgcgcacagc accatgaccg ggtggtagtc atctgcccgg ggcgtattga gagctacgta
     7681 aaatatgcgg aactggccta tgacctgttc catttggggt ttgatgtctt aatcatcgac
     7741 catcgcgggc agggacgttc cggtcgcctg ttagccgatc cgcatctcgg gcatgttaat
     7801 cgctttaatg attatgttga tgatctggcg gcattctggc agcaggaggt tcagcccggt
     7861 ccgtggcgta aacgctatat actggcacat tcgatgggcg gtgcgatctc cacattattt
     7921 ctgcaacgcc atccaggtgt atgtgacgcc attgcgctaa ctgcgccaat gtttgggatc
     7981 gtgattcgta tgccgtcatt tatggcacgg cagatcctca actgggccga agcgcatcca
     8041 cgtttccgtg atggctatgc aataggcacc gggcgctggc gcgcgttgcc gtttgctatc
     8101 aacgtactga cccacagcag acagcgatat cgacgtaact tacgcttcta tgctgatgac
     8161 ccaacgattc gcgtcggtgg gccgacctac cattgggtac gcgaaagtat tctggctggc
     8221 gaacaggtgt tagccggtgc gggtgatgac gccacgccaa cgcttctctt acaagctgaa
     8281 gaggaacgcg tggtggataa ccgcatgcat gaccgttttt gtgaactccg caccgccgcg
     8341 ggccatcctg tcgaaggagg acggccgttg gtaattaaag gtgcttacca tgagatcctt
     8401 tttgaaaagg acgcaatgcg ctcagtcgcg ctccacgcca tcgttgattt tttcaacagg
     8461 cataactcac ccagcggaaa ccgctctaca gaggtttaaa tttcttatgt accaggttgt
     8521 tgcgtctgat ttagatggca cgttactttc tcccgaccat acgttatccc cttacgccaa
     8581 agaaactctg aagctgctca ccgcgcgcgg catcaacttt gtgtttgcga ccggtcgtca
     8641 ccacgttgat gtggggcaaa ttcgcgataa tctggagatt aagtcttaca tgattacctc
     8701 caatggtgcg cgcgttcacg atctggatgg taatctgatt tttgctcata acctggatcg
     8761 cgacattgcc agcgatctgt ttggcgtagt caacgacaat ccggacatca ttactaacgt
     8821 ttatcgcgac gacgaatggt ttatgaatcg ccatcgcccg gaagagatgc gcttttttaa
     8881 agaagcggtg ttcaaatatg cgctgtatga gcctggatta ctggagccgg aaggcgtcag
     8941 caaagtgttt ttcacctgcg attcccatga acaactgctg ccgctggagc aggcgattaa
     9001 cgctcgctgg ggcgatcgcg tcaacgtcag tttctctacc ttaacctgtc tggaagtgat
     9061 ggcgggcggc gtgtcgaaag gccatgcgct ggaagcagtg gcgaagaaac tgggctacag
     9121 cctgaaggat tgtattgcgt ttggtgacgg gatgaacgac gccgaaatgc tgtcgatggc
     9181 ggggaaaggc tgcattatgg gcagtgcgca ccagcgtctg aaagaccttc atcccgagct
     9241 ggaagtgatt ggtactaatg ccgacgacgc ggtgccgcat tatctgcgta aactctattt
     9301 atcgtaatcg ttctttattt ggtcagttgt caacctgata cttcgctaca atggatactc
     9361 gttaatcaaa gagttttcca ttgtggcgct acttatcatc accacgattc tgtgggcctt
     9421 ctcctttagc ttttatggcg agtaccttgc ggggcacgtc gatagctatt ttgcggtgct
     9481 ggtgcgcgtt ggcctggcgg cactcgtttt tctgccgttt ctgcgtaccc gtggcaatag
     9541 cctgaaaacg gtcggcctgt atatgttggt gggcgcgatg cagctcggcg tgatgtatat
     9601 gctgagtttc cgcgcgtatc tctacctgac ggtctccgaa ctgctgctgt tcaccgtact
     9661 gacgccgctc tacatcacgc tgatttatga catcatgagt aagcgccgtc tgcgctgggg
     9721 ctatgccttt agcgccttgt tggcggtgat tggtgccggg attattcgct atgatcaggt
     9781 caccgaccat ttctggactg gcttgctgct ggtgcaactc tccaatatca cttttgccat
     9841 cggcatggtg ggttacaaac gcctgatgga aactcgcccg atgccacagc ataacgcctt
     9901 tgcgtggttc tatcttggcg cgtttctggt ggcggtaatt gcatggttct tgctgggaaa
     9961 tgcgcagaaa atgccgcaaa ccacgttgca atggggcatt ctggtgtttc ttggcgtggt
    10021 ggcttccggg attggctact ttatgtggaa ctacggcgcg acgcaggtgg acgccggaac
    10081 gctgggcatt atgaataata tgcacgttcc ggcagggctg ctggtaaacc tggctatctg
    10141 gcaccaacag ccgcactggc caacgttcat tacaggcgcg ctggtgatcc tggcctcact
    10201 gtgggtgcat cgtaagtggg tcgctccgcg ctcttcacaa acggcagatg atcgcaggcg
    10261 tgattgcgcg ctgagcgaat aaatgcttct gtaatcggct gacgctgctc gccatcgcgc
    10321 acggcggcgt acagtcggct ccacaagcct tcgcccaggg ttttggtcac caccagaccc
    10381 tggcgctcaa aactctctac tacccaatgc ggtagcgcgg caatacccat ccgcgcggca
    10441 accatctgaa tcaacaataa ggtgttatcg acgcttttca gtgacgggct gacgcctgcc
    10501 ggctgaagaa aatgccgcca gacatccagt cgactacgct gcaccggata aatcaacagc
    10561 gtctcgctgg cgagatcttc cggtgtaatt cgcgttttcg ccgccagtgg atggtcaggt
    10621 gctaacacca gacgcacttc atagtcgaac atcggcgaat aatgcaggcc actgcgcggc
    10681 aggatatcgg aggtcatcac cagatccagc tcgccctgtt gcaacgcggg ctgcgggtca
    10741 aacgtcacgc ccgatttaaa atccatctct acctgcggcc agttcttatg gaaattttct
    10801 aacgcgggcg tcagccactg aatacagcta tggcactcaa tggcaatgcg cagacgcgtc
    10861 tgctgcggtt cattgcaggc ttgcagtgcc tggctaattt gcggcagcac ctggtttgcc
    10921 agttgcaaca ggatttctcc ctgcggtgta aagcgtagcg gctggctctt acgcacaaat
    10981 agccggaagc caaggcgttg ttccagatcg ctaaactggt gagacagggc ggattgcgtc
    11041 tgatgcaacg tcgccgcagc ggctgcgaga gagccgcagt tccgcaacgc ttgtagcgtt
    11101 ttcaggtgtt ttacttcgat catgaaagtc cttcacttcg gcatgaataa tttgcgcttg
    11161 aggaatatac agtaaccgcc aattatggat gtgtaaacat ctggacggct aaatactaaa
    11221 aattccataa ggggcatata atgacaattc ttaatcacac cctcggtttc cctcgcgttg
    11281 gcctgcgtcg cgagctgaaa aaagcgcaag aaagttattg ggcggggaac tccacgcgtg
    11341 aagaactgct gacagtaggg cgtgaactgc gtgctcgtca ctgggatcaa caaaagcaag
    11401 cgggtatcga cctgctgccg gtgggcgatt ttgcctggta cgatcatgtt ctgaccacca
    11461 gtctgctgct gggtaatgtt ccgccacgtc atcagaacaa agacggttcg gtagatatcg
    11521 acaccctgtt ccgtattggt cgtggacgtg cgccgactgg cgaacctgcg gcggcagcgg
    11581 aaatgaccaa atggtttaac accaactatc actacatggt gccggaattc gttaaaggcc
    11641 aacagttcaa actgacctgg acgcagctgc tggaggaagt ggacgaggcg ctggcgctgg
    11701 gccacaacgt taagcctgtg ctgctggggc cggttactta cctgtggctg gggaaagtga
    11761 aaggtgaaca gtttgaccgc cttagcctgc tgaacgacat tctgccggtt tatcagcaag
    11821 tgctggcaga actggcgaaa cgcggcatcg agtgggtaca gattgatgaa cccgcgctgg
    11881 tactggaact accacaggcg tggctggacg catacaaacc cgcttacgac gcgctccagg
    11941 gacaggtgaa actgctgctg accacctatt ttgaaggcgt aacgccaaat ctcgacacga
    12001 ttactgcgct gcctgttcag ggtctgcatg ttgacctcgt acatggtaaa gatgacgttg
    12061 ctgaactgca caagcgcctg ccttctgact ggttgctgtc tgcgggtctg atcaatggtc
    12121 gtaacgtctg gcgcgccgat cttaccgaga aatatgcgca aattaaggac attgtcggca
    12181 aacgtgattt gtgggtggca tcttcctgct cgttgctgca cagccccatc gacctgagcg
    12241 tggaaacgcg tcttgatgca gaagtgaaaa gctggtttgc cttcgcccta caaaaatgcc
    12301 atgaactggc actgctgcgc gatgcgctga acagtggtga cacggcagct ctggcagagt
    12361 ggagcgcccc gattcaggca cgtcgtcact ctacccgcgt acataatccg gcggtagaaa
    12421 agcgtctggc ggcgatcacc gcccaggaca gccagcgtgc gaatgtctat gaagtgcgtg
    12481 ccgaagccca gcgtgcgcgt tttaaactgc cagcgtggcc gaccaccacg attggttctt
    12541 tcccgcaaac cacggaaatt cgtaccctgc gtctggattt caaaaagggc aatctcgacg
    12601 ccaataacta ccgcacgggc attgcggaac atatcaggca ggccattgtt gaacaggaac
    12661 gtttgggact ggatgtgctg gtacatggcg aggccgagcg taatgacatg gtggaatact
    12721 ttggcgagca cctcgacggg tttgtcttta cgcaaaacgg ttgggtacag agctacggtt
    12781 cccgctgcgt gaagccaccg attgttattg gtgacgttag tcgcccggca ccgattaccg
    12841 tggagtgggc gaagtatgcg caatcgctga ccgacaaacc ggtgaaaggg atgctgacgg
    12901 ggccggtgac catactctgc tggtcgttcc cgcgtgaaga tgtcagccgt gaaaccatcg
    12961 ccaaacagat tgcgctggcg ctgcgtgatg aggtggccga tctggaagcg gcagggattg
    13021 gtatcatcca gattgacgaa ccggcgctgc gcgaaggttt accactgcgt cgtagcgact
    13081 gggacgccta tctccagtgg ggcgtagagg ccttccgtat caacgccgcc gtggcgaaag
    13141 atgacacaca aatccacact cacatgtgtt attgcgagtt caacgacatc atggattcca
    13201 ttgcggcgct ggatgcagac gtcatcacca tcgaaacctc gcgttccgac atggagttgc
    13261 tggagtcgtt tgaagagttt gattatccga atgaaatcgg tccaggcgtc tatgacattc
    13321 actcgccaaa cgtaccgagc gtggaatgga ttgaagccct gctgaagaaa gcggcaaaac
    13381 gcattccggc agagcgcctg tgggtcaacc cagactgtgg cctgaaaacg cgcggctggc
    13441 cagaaacccg cgcggcactg gcgaacatgg tgcaggcggc gcagaatttg cgtctggggt
    13501 aaatccaaac cgggtggtaa taccacccgg tcttttctca ttacagcgac ttcttcccac
    13561 catactgctt aaaccattcc agcatacgct gccagccatc tttcgcagat tcggcatgat
    13621 agctcgggcg ataatcagcg ttgaatgcat gcccggcatc cgggtagacg ataatctctg
    13681 ctttcgcatt agcagcccgc agcgcctggc gcatcgtttc aacgctctcc tgcggaatgc
    13741 tgttatcctg accgccatat aagccgagaa ccggcgcgtt aagatcggtt gcgatatcaa
    13801 caggttgttt cggtgaattc agcgacttat cgcccgtcag tttgccgtac cacgccactg
    13861 cggcttttag ctgtggatta tgcgcggcat acagccaggt gatacgtcca ccccagcaga
    13921 atccggtgat cattaaacga tgaacatcgc cgccgttgcg ggaagcccaa ctggcgacat
    13981 gatcgagatc ggccagcacc tgcgagtcag gcacttttgc taccagaccg ctaagcaacg
    14041 tggggatatc ggcaaaatca ttcggatcgc cttcgcggaa gtaaagttca ggtgcgatag
    14101 ccagataccc ctccagcgcc agacggcgac aaatgtcgcg gatatgttca tgcacgccaa
    14161 aaatttcctg cactacaatg accactggca gtgggccatc gctttgcttt ggtctggcat
    14221 ggtaagcagg catgttatcc ccttgtgaag ggatagaggt gaagcccgcc acaatcgcgt
    14281 cgtccggggt ctgaacgatg gtcgaagcga gaggcgatgc agcaggtgca aatccagatt
    14341 gttgtgttgt tgccatggta ttctccgtac ccttataaaa atgttgcgca atgttaacta
    14401 tagtcagcat gcaacaaatc acattgcctg aatcggctca tcttttatgc agtcctgcag
    14461 aatgaagagt gatttatgtg atttgcatca cttttggtgg gtaaatttat gcaacgcatt
    14521 tgcgtcatgg tgatgagtat cacgaaaaaa tgttaaaccc ttcggtaaag tgtctttttg
    14581 cttcttctga ctaaaccgat tcacagagga gttgtatatg tccaagtctg atgtttttca
    14641 tctcggcctc actaaaaacg atttacaagg ggctacgctt gccatcgtcc ctggcgaccc
    14701 ggatcgtgtg gaaaagatcg ccgcgctgat ggataagccg gttaagctgg catctcaccg
    14761 cgaattcacc acctggcgtg cagagctgga tggtaaacct gttatcgtct gctctaccgg
    14821 tatcggcggc ccgtctacct ctattgctgt tgaagagctg gcacagctgg gcattcgcac
    14881 cttcctgcgt atcggtacaa cgggcgctat tcagccgcat attaatgtgg gtgatgtcct
    14941 ggttaccacg gcgtctgtcc gtctggatgg cgcgagcctg cacttcgcac cgctggaatt
    15001 cccggctgtc gctgatttcg aatgtacgac tgcgctggtt gaagctgcga aatccattgg
    15061 cgcgacaact cacgttggcg tgacagcttc ttctgatacc ttctacccag gtcaggaacg
    15121 ttacgatact tactctggtc gcgtagttcg tcactttaaa ggttctatgg aagagtggca
    15181 ggcgatgggc gtaatgaact atgaaatgga atctgcaacc ctgctgacca tgtgtgcaag
    15241 tcagggcctg cgtgccggta tggtagcggg tgttatcgtt aaccgcaccc agcaagagat
    15301 cccgaatgct gagacgatga aacaaaccga aagccatgcg gtgaaaatcg tggtggaagc
    15361 ggcgcgtcgt ctgctgtaat tctcttctcc tgtctgaagg ccgacgcgtt cggccttttg
    15421 tatttttgcg tagcgcctcg caggaaatgc ctttccaact ggacgtttgt acagcacaat
    15481 tctattttgt gcgggtaagt tgttgcgtca ggaggcgttg tggatttctc aatcatggtt
    15541 tacgcagtta ttgcgttggt gggtgtggca attggctggc tgtttgccag ttatcaacat
    15601 gcgcagcaaa aagccgagca attagctgaa cgtgaagaga tggtcgcgga gttaagcgcg
    15661 gcaaaacaac aaattaccca aagcgagcac tggcgtgcag agtgcgagtt actcaataac
    15721 gaagtgcgca gcctgcaaag tattaacacc tctctggagg ccgatctgcg tgaagtaacc
    15781 acgcggatgg aagccgcaca gcaacatgct gacgataaaa ttcgccagat gattaacagc
    15841 gagcagcgcc tcagtgagca gtttgaaaac ctcgccaacc gtatttttga gcacagcaat
    15901 cgccgggttg atgagcaaaa ccgtcagagt ctgaacagcc tgttgtcgcc gctacgtgaa
    15961 caactggacg gtttccgccg tcaggttcag gacagcttcg gtaaagaagc acaagaacgc
    16021 cataccctga cccacgaaat tcgcaatctc cagcaactca acgcgcaaat ggcccaggaa
    16081 gcgatcaacc tgacgcgcgc gctgaaaggc gacaataaaa cccagggcaa ctggggcgag
    16141 gtagtattga cgcgggtgct ggaggcttcc ggtctgcgtg aagggtatga atatgaaacc
    16201 caggtcagca tcgaaaatga cgcccgctcg cggatgcagc cggatgtcat cgtgcggctg
    16261 ccgcagggaa aagatgtggt gatcgacgcc aaaatgacgc tggtcgccta tgaacgctat
    16321 tttaacgccg aagacgacta cacccgcgaa agcgcgctac aggaacatat cgcgtcggtg
    16381 cgtaaccata tccgtttgct gggacgcaaa gattatcaac agctgccggg gctgcgaact
    16441 ctggattacg tgctgatgtt tattcccgtt gaacccgctt tttcactggc gcttgaccgc
    16501 cagccggagc tgatcaccga agcgttgaaa aacaatatca tgctggttag cccgactacg
    16561 ctgctggtgg cgttgcgtac tattgccaac ctgtggcgtt atgagcatca aagccgcaac
    16621 gcccagcaaa tcgccgatcg tgccagcaag ctgtacgaca agatgcgttt gttcatcgat
    16681 gacatgtccg cgattggtca aagtctcgac aaagcgcagg ataactaccg ccaggcaatg
    16741 aaaaaactct cttcaggtcg cggaaatgtg ctggcgcagg cagaagcgtt tcgcggttta
    16801 ggagtagaaa ttaaacgcga gattaatccg gatttggctg aacaggcggt gagccaggat
    16861 gaagagtatc gacttcggtc ggttccggag cagccgaatg atgaagctta tcaacgcgat
    16921 gatgaatata atcagcagtc gcgctagccc attaggggta gttaagccgg gtagaaatct
    16981 agggcatcga cgcccaatct gttacacttc tggaacaatt ttttgatgag caggcattga
    17041 gatggtggat aagtcacaag aaacgacgca ctttggtttt cagaccgtcg cgaaggaaca
    17101 aaaagcggat atggtcgccc acgttttcca ttccgtggca tcaaaatacg atgtcatgaa
    17161 tgatttgatg tcatttggta ttcatcgttt gtggaagcga ttcacgattg attgcagcgg
    17221 cgtacgccgt gggcagaccg tgctggatct ggctggtggc accggcgacc tgacagcgaa
    17281 attctcccgc ctggtcggag aaactggcaa agtggtcctt gctgatatca atgaatccat
    17341 gctcaaaatg ggacgcgaga agctgcgtaa tatcggtgtg attggcaacg ttgagtatgt
    17401 tcaggcgaac gctgaggcgc tgccgttccc ggataacacc tttgattgca tcaccatttc
    17461 gtttggtctg cgtaacgtca ccgacaaaga taaagcactg cgttcaatgt atcgcgtgct
    17521 gaaacccggc ggccgcctgc tggtgcttga gttctcgaag ccaattatcg agccgctgag
    17581 caaagcctat gatgcatact ccttccatgt gctgccgcgt attggctcac tggtcgcgaa
    17641 cgacgccgac agctaccgtt atctggcaga atccatccgt atgcatcccg atcaggatac
    17701 cctgaaagcc atgatgcagg atgccggatt cgaaagtgtc gactactaca atctgacggc
    17761 gggggttgtg gcgctgcatc gtggttataa gttctgacag gagaccggaa atgcctttta
    17821 aacctttagt gacggcagga attgaaagtc tgctcaacac cttcctgtat cgctcacccg
    17881 cgctgaaaac agcccgctcg cgtctgctgg gtaaagtatt gcgcgtggag gtaaaaggct
    17941 tttcgacgtc attgattctg gtgttcagcg aacgccaggt tgatgtactg ggcgaatggg
    18001 caggcgatgc tgactgcacc gttatcgcct acgccagtgt gttgccgaaa cttcgcgatc
    18061 gccagcagct taccgcactg attcgcagtg gtgagctgga agtgcagggc gatattcagg
    18121 tggtgcaaaa cttcgttgcg ctggcagatc tggcagagtt cgaccctgcg gaactgctgg
    18181 ccccttatac cggtgatatc gccgctgaag gaatcagcaa agccatgcgc ggaggcgcaa
    18241 agttcctgca tcacggcatt aagcgccagc aacgttatgt ggcggaagcc attactgaag
    18301 agtggcgtat ggcacccggt ccgcttgaag tggcctggtt tgcggaagag acggctgccg
    18361 tcgagcgtgc tgttgatgcc ctgaccaaac ggctggaaaa actggaggct aaatgacgcc
    18421 aggtgaagta cggcgcctat atttcatcat tcgcactttt ttaagctacg gacttgatga
    18481 actgatcccc aaaatgcgta tcaccctgcc gctacggcta tggcgatact cattattctg
    18541 gatgccaaat cggcataaag acaaactttt aggtgagcga ctacgactgg ccctgcaaga
    18601 actggggccg gtttggatca agttcgggca aatgttatca acccgccgcg atctttttcc
    18661 accgcatatt gccgatcagc tggcgttatt gcaggacaaa gttgctccgt ttgatggcaa
    18721 gctggcgaag cagcagattg aagctgcaat gggcggcttg ccggtagaag cgtggtttga
    18781 cgattttgaa atcaagccgc tggcttctgc ttctatcgcc caggttcata ccgcgcgatt
    18841 gaaatcgaat ggtaaagagg tggtgattaa agtcatccgc ccggatattt tgccggtcat
    18901 taaagcggat ctgaaactta tctaccgtct ggctcgctgg gtgccgcgtt tgctgccgga
    18961 tggtcgccgt ctgcgcccaa ccgaagtggt gcgcgagtac gaaaagacct tgattgatga
    19021 actgaatttg ctgcgggaat ctgccaacgc cattcagctt cggcgcaatt ttgaagacag
    19081 cccgatgctc tacatcccgg aagtttaccc tgactattgt agtgaaggga tgatggtgat
    19141 ggagcgcatt tacggcattc cggtgtctga tgttgcggcg ctggagaaaa acggcactaa
    19201 catgaaattg ctggcggaac gcggcgtgca ggtgttcttc actcaggtct ttcgcgacag
    19261 ctttttccat gccgatatgc accctggcaa catcttcgta agctatgaac acccggaaaa
    19321 cccgaaatat atcggcattg attgcgggat tgttggctcg ctaaacaaag aagataaacg
    19381 ctatctggca gaaaacttta tcgccttctt taatcgcgac tatcgcaaag tggcagagct
    19441 acacgtcgat tcaggctggg tgccaccaga taccaacgtt gaagagttcg aatttgccat
    19501 tcgtacggtc tgtgaaccta tctttgagaa accgctggcc gaaatttcgt ttggacatgt
    19561 actgttaaat ctgtttaata cggcgcgtcg cttcaatatg gaagtgcagc cgcaactggt
    19621 gttactccag aaaaccctgc tctatgtcga aggggtagga cgccagcttt atccgcagct
    19681 cgatttatgg aaaacagcga agcctttcct ggagtcgtgg attaaagatc aggtcggtat
    19741 tcctgcgctg gtgagagcat ttaaagaaaa agcgccgttc tgggtcgaaa aaatgccaga
    19801 actgcctgaa ttggtttacg acagtttgcg ccagggcaag tatttacagc acagtgttga
    19861 taagattgcc cgcgagcttc agtcaaatca tgtacgtcag ggacaatcgc gttattttct
    19921 cggaattggc gctacgttag tattaagtgg cacattcttg ttggtcagcc gacctgagtg
    19981 ggggctgatg cccggctggt taatggcagg tggtctgatc gcctggtttg tcggttggcg
    20041 caaaacacgc tgattttttc atcgctcaag gcgggccgtg taacgtataa tgcggctttg
    20101 tttaatcatc atctaccaca gaggaacatg tatgggtggt atcagtattt ggcagttatt
    20161 gattattgcc gtcatcgttg tactgctttt tggcaccaaa aagctcggct ccatcggttc
    20221 cgatcttggt gcgtcgatca aaggctttaa aaaagcaatg agcgatgatg aaccaaagca
    20281 ggataaaacc agtcaggatg ctgattttac tgcgaaaact atcgccgata agcaggcgga
    20341 tacgaatcag gaacaggcta aaacagaaga cgcgaagcgc cacgataaag agcaggtgta
    20401 atccgtgttt gatatcggtt ttagcgaact gctattggtg ttcatcatcg gcctcgtcgt
    20461 tctggggccg caacgactgc ctgtggcggt aaaaacggta gcgggctgga ttcgcgcgtt
    20521 gcgttcactg gcgacaacgg tgcagaacga actgacccag gagttaaaac tccaggagtt
    20581 tcaggacagt ctgaaaaagg ttgaaaaggc gagcctcact aacctgacgc ccgaactgaa
    20641 agcgtcgatg gatgaactac gccaggccgc ggagtcgatg aagcgttcct acgttgcaaa
    20701 cgatcctgaa aaggcgagcg atgaagcgca caccatccat aacccggtgg tgaaagataa
    20761 tgaagctgcg catgagggcg taacgcctgc cgctgcacaa acgcaggcca gttcgccgga
    20821 acagaagcca gaaaccacgc cagagccggt ggtaaaacct gctgcggacg ctgaaccgaa
    20881 aaccgctgca ccttcccctt cgtcgagtga taaaccgtaa acatgtctgt agaagatact
    20941 caaccgctta tcacgcatct gattgagctg cgtaagcgtc tgctgaactg cattatcgcg
    21001 gtgatcgtga tattcctgtg tctggtctat ttcgccaatg atatctatca cctggtatcc
    21061 gcgccattga tcaagcagtt gccgcaaggt tcaacgatga tcgccaccga cgtggcctcg
    21121 ccgttcttta cgccgatcaa gctgaccttt atggtgtcgc tgattctgtc agcgccggtg
    21181 attctctatc aggtgtgggc atttatcgcc ccagcgctgt ataagcatga acgtcgcctg
    21241 gtggtgccgc tgctggtttc cagctctctg ctgttttata tcggtatggc attcgcctac
    21301 tttgtggtct ttccgctggc atttggcttc cttgccaata ccgcgccgga aggggtgcag
    21361 gtatccaccg acatcgccag ctatttaagc ttcgttatgg cgctgtttat ggcgtttggt
    21421 gtctcctttg aagtgccggt ggcaattgtg ctgctgtgct ggatggggat tacctcgcca
    21481 gaagacttac gcaaaaaacg cccgtatgtg ctggttggtg cattcgttgt cgggatgttg
    21541 ctgacgccgc cggatgtctt ctcgcaaacg ctattggcga tcccgatgta ctgtctgttt
    21601 gaaatcggtg tcttcttctc acgcttttac gttggtaaag ggcgaaatcg ggaagaggaa
    21661 aacgacgctg aagcagaaag cgaaaaaact gaagaataaa ttcaaccgcc cgtcagggcg
    21721 gttgtcatat ggagtacagg atgtttgata tcggcgttaa tttgaccagt tcgcaatttg
    21781 cgaaagaccg tgatgatgtt gtagcgcgcg cttttgacgc gggagttaat gggctactca
    21841 tcaccggcac taacctgcgt gaaagccagc aggcgcaaaa gctggcgcgt cagtattcgt
    21901 cctgttggtc aacggcgggc gtacatcctc acgacagcag ccagtggcaa gctgcgactg
    21961 aagaagcgat tattgagctg gccgcgcagc cagaagtggt ggcgattggt gaatgtggtc
    22021 tcgactttaa ccgcaacttt tcgacgccgg aagagcagga acgcgctttt gttgctcagc
    22081 tacgcattgc cgcagaatta aacatgccgg tatttatgca ctgtcgcgat gcccacgagc
    22141 ggtttatgac attgctggag ccgtggctgg ataaactgcc tggtgcggtt cttcattgct
    22201 ttaccggcac acgcgaagag atgcaggcgt gcgtggcgcg tggaatttat atcggcatta
    22261 ccggttgggt ttgcgatgaa cgacgcgggc tggagctgcg ggaattgttg ccgttgattc
    22321 cggcggagaa attgctgatc gaaactgatg cgccgtatct gctcccgcgc gatctcacgc
    22381 caaagccatc atcccggcgc aacgagccag cccatctgcc ccatattttg caacgtattg
    22441 cgcactggcg tggagaagat gccgcatggc tggctgccac cacggatgct aatgtcaaaa
    22501 cactgtttgg gattgcgttt tagagtttgc ggaactcggt gttcttcaca ctgtgcttaa
    22561 tctctttatt aataagatta agcaatagca tggagcgagc ctcaccatcg ggttcggtga
    22621 aaatggcctg aaagccttcg aacgcgcctt cggtaataat caccttatca cccggataag
    22681 gggttgccgg atcgacaatg tctttcggtt tatataccga tagctgatga atcaccgccg
    22741 atgggactat cgctggcgac gcgccaaagc gcacgaagtg gctgacaccg cgggtcgcgt
    22801 tgatagtcgt ggtatgaatc acttctggat caaattcgac aaacaggtag ttgggaaaca
    22861 atggctcact gactgcagta cgttttccac gcacgatttt ttccagggtg atcatcggtg
    22921 ccaggcaatt cacagcctgt ctttcgaggt gttcctgggc acgttgaagt tgcccgcgct
    22981 tgcagtacag taaataccag gattgcataa tgactcttat ccgtttaatc ggggcgcaag
    23041 gatagcaaaa gctttacgct aagttaatta tattccccgg tttgcgttat accgtcagag
    23101 ttcacgctaa tttaacaaat ttacagcatc gcaaagatga acgccgtata atgggcgtag
    23161 attaagaggc tacaatggac gccatgaaat ataacgattt acgcgacttc ttgacgctgc
    23221 ttgaacagca gggtgagcta aaacgtatca cgctcccggt ggatccgcat ctggaaatca
    23281 ctgaaattgc tgaccgcact ttgcgtgccg gtgggcctgc gctgttgttc gaaaacccta
    23341 aaggctactc aatgccggtg ctgtgcaacc tgttcggtac gccaaagcgc gtggcgatgg
    23401 gcatggggca ggaagatgtt tcggcgctgc gtgaagttgg taaattattg gcgtttctga
    23461 aagagccgga gccgccaaaa ggtttccgcg acctgtttga taaactgccg cagtttaagc
    23521 aagtattgaa catgccgaca aagcggctgc gtggtgcgcc ctgccaacaa aaaatcgtct
    23581 ctggcgatga cgtcgatctc aatcgcattc ccattatgac ctgctggccg gaagatgccg
    23641 cgccgctgat tacctggggg ctgacagtga cgcgcggccc gcataaagag cggcagaatc
    23701 tgggcattta tcgccagcag ctgattggta aaaacaaact gattatgcgc tggctgtcgc
    23761 atcgcggcgg cgcgctggat tatcaggagt ggtgtgcggc gcatccgggc gaacgtttcc
    23821 cggtttctgt ggcgctgggt gccgatcccg ccacgattct cggtgcagtc acccccgttc
    23881 cggatacact ttcagagtat gcgtttgccg gattgctgcg cggtaccaag accgaagtgg
    23941 tgaagtgtat ctccaatgac cttgaagtgc ccgccagtgc ggagattgtg ctggaagggt
    24001 atatcgaaca aggtgaaacg gcgccggaag ggccgtatgg cgaccacacc ggttactata
    24061 acgaagtcga tagtttcccg gtatttaccg tgacgcatat tacccagcgt gaagatgcga
    24121 tttaccattc cacctatacc gggcgtccgc cagatgagcc cgcggtactg ggcgtggcgt
    24181 tgaacgaagt gtttgtaccg attctgcaaa aacagttccc ggaaattgtc gatttttacc
    24241 tgccgccgga aggctgttct tatcgtctgg cggtagtgac gatcaaaaaa cagtacgccg
    24301 gacacgcgaa gcgcgtcatg atgggcgtct ggtcgttctt acgccagttt atgtacacta
    24361 aatttgtgat cgtttgcgat gatgacgtca acgcacgcga ctggaacgat gtgatttggg
    24421 cgattaccac ccgtatggac ccggcgcggg atactgttct ggtagaaaat acgcctattg
    24481 attatctgga ttttgcctcg cctgtctccg ggctgggttc aaaaatgggg ctggatgcca
    24541 cgaataaatg gccgggggaa acccagcgtg aatggggacg tcccatcaaa aaagatccag
    24601 atgttgtcgc gcatattgac gccatctggg atgaactggc tatttttaac aacggtaaaa
    24661 gcgcctgatg cgcgtttgtt ttgccctatt tatcgatccg acagagaaag cgcatgacaa
    24721 ccttaagctg taaagtgacc tcggtagaag ctatcacgga taccgtatat cgtgtccgca
    24781 tcgtgccaga cgcggccttt tcttttcgtg ctggtcagta tttgatggta gtgatggatg
    24841 agcgcgacaa acgtccgttc tcaatggcct cgacgccgga tgaaaaaggg tttatcgagc
    24901 tgcatattgg cgcttctgaa atcaaccttt acgcgaaagc agtcatggac cgcatcctca
    24961 aagatcatca aatcgtggtc gatattcccc acggagaagc gtggctgcgc gatgatgaag
    25021 agcgtccgat gattttgatt gcgggcggca ccgggttctc ttatgcccgc tcgattttgc
    25081 tgacagcgtt ggcgcgtaac ccaaaccgtg atatcaccat ttactggggc gggcgtgaag
    25141 agcagcatct gtatgatctc tgcgagcttg aggcgctttc gttaaagcac cctggtctgc
    25201 aagtggtgcc ggtggttgaa caaccggaag caggctggcg tgggcgtact ggtaccgtgt
    25261 taacggcggt attgcaggat catggtacgc tggcagagca tgatatctat attgccggac
    25321 gttttgagat ggcgaaaatt gcccgtgacc tgttttgcag tgagcgtaat gcgcgggaag
    25381 atcgcctgtt tggcgatgcg tttgcattta tctgagatat aaaaaaaccc gcccctgaca
    25441 ggcgggaaga acggcaacta aaactttatt cagtggcatt tagatctatg tcgcatccgg
    25501 caatcggtgc acgatgcctg atgcgacgct ggcgcgtctt atcaggccta cattggtgca
    25561 ggatcggtag accggataag gcgttcacgc cgcatccggc aagtggttaa acccgctcaa
    25621 acaccgtcgc aataccctga cccagaccga tacacatcgt cgccagacca aactgaacgt
    25681 ctttgcgttc catcagattc agcagcgtgg tgctgatacg cgcaccggaa cagcccagcg
    25741 gatgacccag cgcgatcgca ccaccgttga ggttgatctt ctcgtcaatc tgctcaatca
    25801 gtcccagatc tttaatacat ggcaggatct gcgcggcaaa ggcttcgttc atctcaaaca
    25861 cgccgatatc gctggcagaa agccccgctt ttttcagcgc cagcttcgag gccggaaccg
    25921 ggccgtaacc cataatcgat gggtcacaac caacgaccgc catcgaacgc acacgagcgc
    25981 gcggcttaag acctaattca tgggcgcggc tttcactcat caccagcatg gcagctgcgc
    26041 catcggaaag tgcagaagat gtgcccgccg ttaccgtacc gtttactgga tcaaacgccg
    26101 gacgcagcgt ggcgagggct tccacggtgg tttccgggcg aatcacttcg tcgtaattaa
    26161 actgcttcag gacgccgtcg gcatcgtgac caccagttgg gatgatttca tttttaaatg
    26221 cggccgactg cgtggcggcc caggcgcggg cgtgtgaccg cgcggcaaag gcatcctgca
    26281 tttcacggct gataccgtgc atacgcgcca gcatttctgc tgttaagccc atcatgcccg
    26341 ccgctttggc gacattgcgg ctcaggccgg ggtgaaaatc gacgccgtga ctcatcggca
    26401 catggcccat atgctccacg ccgccaacca gacatgcctg cgcatcgccg gtcataatca
    26461 tccgtgctgc gtcatgcagt gcctgcatgg atgaaccaca caagcgattg acggtaaccg
    26521 ccgggacaga gtgtggtact tctgccagca gcgccgcgtt acgggcgatg ttaaaaccct
    26581 gctccagcgt ctgctgcaca caaccccagt aaatatcgtc gagggccgcc gcttccagcg
    26641 ccgggttacg cgccagcagg ctacgcatta aatgagcgga gagatcttct gcacgcacgt
    26701 tacgaaaagc accgcccttc gaacggccca tcggggtgcg aattgcatcg acaatgacaa
    26761 cctgttccat tgtgactcct taagccgttt tcaggtcgcc aaccggacgg gctggctcaa
    26821 ccggaggata gtacggttcg ttatgacgcg ctttattacg cagaccttcc ggcacttcat
    26881 acagcgggcc gaggtgctga tattgctgtg ccatatcgag gtattttgcg ctaccgaggg
    26941 tgtccagcca gcggaacgcg ccgccgtgga acggagggaa gcccaggccg tagaccagcg
    27001 ccatatccgc ttccgccgga gtggcgataa tgccttcctc cagacagcgc accacttcgt
    27061 tgaccatcgg gatcatcatg cgggcgataa tctcctcttc gctgaaatcg cgcttcggct
    27121 ggctcacttc tgccagcagg tcttcaacgg cggcgtcttc ttctttcttc ggcttacctt
    27181 tgctgtcttc tttataacgc cagaaaccga ggccgttctt ctgaccaaag cggttggcat
    27241 caaacagcgc gtcgatggca tcgcggtaat ctttctgcat cctctgcggg aagcctgctg
    27301 ccatgacagc ctgggcatga tgcgcggtgt caatgcccac gacatccagc agatatgccg
    27361 gacccatcgg ccagccaaac tgtttttcca tcactttgtc gatcttgcgg aaatccgcgc
    27421 cgtcgcgcag cagctggctg aaaccggcga aatacgggaa cagcacgcgg ttaacaaaga
    27481 agccggggca gtcgttaacc acaatcggcg tcttgcccat cttgctcgcc caggcgacaa
    27541 ctttcgcgat ggtttcgtcg gagcttttct cgccgcgaat aatttctacc aacggcattc
    27601 ggtggaccgg gttaaagaag tgcatcccgc agaagttttc cgggcgtttc agcgcgttgg
    27661 ccagttcgct gataggaatg gttgaagtgt tagacgccag cacggtatcc tggcgtactt
    27721 tttgttcggt ttctgccagt acggcttttt tcactttcgg gttttcaaca accgcttcta
    27781 ccacaatatc cacgcggtca aatccggcgt agtcgagcgt tgggtggatt gtggagatca
    27841 cgccagccag tttcagacca tcgatcttgc cgcgctcaag ctgcttgttc agcagtttcg
    27901 cggcttcggt catgccgagg gttaacgact tgtcgttgat atctttcatg acaaccggca
    27961 cgcctttcca cgcagactgg taagcgatgc cgccgcccat aatgcctgca cccagcaccg
    28021 cggcctgttt cggggtttca acgtctttgg tgagtttctt cgctttgcct tttacatatt
    28081 gatcgttaag gaaaatgccg accagtgcgc gggcttcgtt ggtatgcgcc agcgggacaa
    28141 aacttttgtt ttccaggttt aaggcttctt cacgaccaaa acgggccgca gcttcaatgg
    28201 tttttactgc ggtgatgggg gccggataat gtttccccgc tgtttgtgcg accatccctt
    28261 tagcgatggt gaagctcatg gtggcttcaa tcttgctcag ttttagtggt tccagcttcg
    28321 gctgacgttt tgctttccag tcgaggtcgc cgttaatggc ctggcgtaaa accgcctttg
    28381 cgccttcaac cagtttttct gctttgacta cgccatccac cagaccgatt ttcagcgcct
    28441 gatccgcgcc gacatcttta ccggcggcaa tgatttccag cgcactgtct gcgcccagca
    28501 tacgtggcat acgtacagaa ccgccaaagc ccggcatgat gcccagtttg gtttccggca
    28561 gaccgatgcg cagatccggc gtcgccagac gataatcggt cgccagcacg cattcgcagc
    28621 caccgcccag cgcatagccg ttgacggcag caatggtcgg caccggcaga tcttccaggc
    28681 gattaaacac gctattggca aagtgcagcc actgacttaa ctgttcttca ggaacgagga
    28741 acagggacaa aaattcggtg atatcagcac cgacgataaa ggctgctttg ttcgaacgca
    28801 gcagcagccc ttttagatct gattgctgtt ccagcacgcc gatggcctcg ccgaggctgg
    28861 cgacggtcgc agtgtcgagt ttattaactg aacctggggc atcaaatacc agttcggcaa
    28921 tgccatcttc cagccagtca aggtacaggg tgtcgccttt gtaaagcatg tcagtctcct
    28981 gaatccgcaa ggtgatctgg tcgtaccaga tgcatcgaag tgtgtatttt gtgttaaaaa
    29041 tatgcaaata aaagattaaa gaaatgccga tctgatcacg ctcggcagaa atcacgctct
    29101 ggatgaacga tgtgctaaga tgcggagact taaggtcaaa aaaacagaag ggtaaaaaat
    29161 ggaatcactg gcctcactct ataaaaatca tatagctacc ttacaggaac ggactcgcga
    29221 tgcgctggcg cgcttcaagc tggatgcgtt acttattcac tccggcgaac tgttcaacgt
    29281 ttttctcgac gatcatccct atccgtttaa agtgaacccg caattcaaag cgtgggtgcc
    29341 ggtaactcag gtgccaaact gctggctgct ggtggatggc gtgaacaagc cgaaactgtg
    29401 gttctatctg ccggttgatt actggcacaa cgtcgaaccg ctgccgacct ctttctggac
    29461 tgaagacgtg gaagtgatcg cgctgccgaa agccgatggc attggtagcc tgctgcctgc
    29521 tgcgcgcggg aatatcggtt atatcggtcc ggtgccggaa cgtgcgctgc aactgggtat
    29581 tgaggccagc aatatcaacc cgaaaggggt gatcgactac ctgcattact accgctcctt
    29641 caaaaccgag tacgaactgg cctgtatgcg tgaagcgcag aaaatggcgg tcaacggtca
    29701 tcgcgcggca gaagaagcgt tccgttctgg catgagcgag ttcgatatca acattgccta
    29761 tctgaccgcg accggtcatc gtgataccga cgtaccttac agcaacattg tggcgctcaa
    29821 cgaacacgct gcggtgttgc attacaccaa actggaccac caggcaccgg aagagatgcg
    29881 cagcttcctg ctggatgccg gggccgaata taacggctat gccgctgacc tgacccgtac
    29941 ctggtcggca aaaagtgaca acgattacgc acagctggtg aaagacgtaa atgatgaaca
    30001 actggcgctg atcgcgacca tgaaagctgg cgttagctat gtggattacc acatccagtt
    30061 ccatcagcgc atcgccaaac tgctgcgtaa acatcaaatc atcaccgata tgagtgaaga
    30121 agcgatggtc gaaaacgatc tcaccggacc gtttatgccg cacggtatcg gccatccgct
    30181 gggcctgcag gtgcatgacg tcgccggttt tatgcaggat gatagcggta cgcacctcgc
    30241 ggcaccggca aaatatccgt acctgcgctg cacccgtatt ctccagccgg gcatggtgtt
    30301 aaccatcgaa ccgggtatct acttcattga atcgctactg gcaccgtggc gtgaagggca
    30361 gttcagcaag cacttcaact ggcagaaaat tgaagcactg aaaccgttcg gcggcattcg
    30421 tatcgaagac aacgtggtga tccacgaaaa taacgtggaa aacatgaccc gggatctgaa
    30481 actggcgtga tggaaagctg gttaattcct gcggcaccgg tcacggtcgt tgaagagatc
    30541 aaaaagagcc gcttcataac gatgttggcg cataccgatg gcgttgaggc ggcgaaagcg
    30601 tttgttgaat cggtgcgggc agaacacccc gatgcccgcc accattgcgt ggcgtgggtg
    30661 gcgggtgcgc cggatgattc acaacagctg ggtttctctg acgacgggga gccggcggga
    30721 acggcaggta aaccgatgct cgcccagcta atgggcagcg gcgttgggga aattaccgct
    30781 gtggtggtgc gctactacgg cggcatattg cttggcaccg gtgggttagt gaaagcgtat
    30841 ggcggcggcg tgaatcaggc gctgcgccag ctaacgaccc aacgcaagac gccattaacc
    30901 gaatatactt tgcaatgtga atatcatcag ttaaccggca ttgaagcgtt gctggggcag
    30961 tgtgacggca aaattatcaa cagtgattat caggcattcg ttctgctgcg ggtggcgctt
    31021 ccggcggcga aagtggctga attttccgca aagctggcgg attttagccg tggttcattg
    31081 caattgttag cgattgaaga ataatcccca cttcgttttg cagaactaag gaagcggcag
    31141 agatgcattt tcgcgccatt acccgaatcg ttggactact ggtcatctta ttttcaggga
    31201 ccatgattat ccctgggctg gtagcactca tctaccggga tggagcgggc cgcgctttta
    31261 cccagacctt ttttgtcgcc ctcgccattg gctctatgct gtggtggccg aaccgcaaag
    31321 agaaaggcga acttaaatcc cgtgaggggt ttctgatagt ggtgctgttc tggaccgtgc
    31381 tgggtagcgt cggtgcgctc ccttttatct tctcggaaag cccgaacctc acgattaccg
    31441 atgcgttttt tgaatctttc tctggcctga ccaccaccgg ggccactacg ctggtggggc
    31501 tggattcgct ccctcacgcc atcctctttt atcgccagat gctgcaatgg tttggcggga
    31561 tggggatcat cgtgttagcg gttgcgatac tgcctatcct cggcgtgggt gggatgcagc
    31621 tctatcgcgc agaaatgccc ggcccgctga aagataacaa aatgcgcccg cgaattgcgg
    31681 aaacggcgaa aaccctgtgg ttgatttatg tcttgctgac cgtcgcctgt gcgctggcgt
    31741 tgtggtttgc cggaatggat gcctttgatg ccatcggcca tagctttgcg actatcgcta
    31801 ttggcggctt ctcgacacat gatgccagta tcggttattt cgatagcccg actattaaca
    31861 ctatcattgc tatcttcctg ctgatctccg gctgtaacta cggtctgcac ttttcactgt
    31921 taagtgggcg tagtctgaag gtttattggc gcgatccgga atttcgcatg tttatcggcg
    31981 tacagtttac gctggtggtt atttgtaccc tcgtactgtg gtttcataat gtctacagtt
    32041 cggcgctgat gacaattaac caggcgtttt tcctggtggt gtcgatggcg acaaccgccg
    32101 ggtttacgac tgacagcatt gcccgctggc cgctcttttt gccggtactg cttttatgtt
    32161 cggcttttat cggcggttgt gccgggtcaa cgggcggtgg cctgaaagtg atccgcatcc
    32221 tgctgctgtt taagcagggg aaccgtgagc tgaaacgact ggtgcatccg aacgccgtct
    32281 atagcattaa gctggggaat cgcgcactgc cggaacgtat cctcgaagcc gtttggggat
    32341 ttttctccgc ctatgcattg gtgtttattg tcagtatgct ggcgattatc gccacgggcg
    32401 tggatgactt ttctgccttt gcctcagttg ttgcgacatt gaataacctg gggccagggc
    32461 ttggcgtggt tgctgataac tttaccagta tgaacccggt ggctaaatgg atcctgattg
    32521 ccaacatgct gtttggtcgt ctcgaggtct ttacattgct ggtgctcttt accccgactt
    32581 tctggcgtga atgatggagt aatacgtgaa aacattaatt cttttctcaa caagggacgg
    32641 acaaacgcgc gagattgcct cctacctggc ttcggaactg aaagaactgg ggatccagac
    32701 ggatgtcgcc aatgtgcacc gcattgaaga accacaatgg gaaaattatg accgtgtggt
    32761 cattggtgct tctattcgct atggtcacta ccactctgcg ttccaggaat ttgtcaaaaa
    32821 acatgcgacg cgactgaatt cgatgccgag cgccttttat tccgtaaacc tggtggcgcg
    32881 caaaccggag aagcgtactc cacagaccaa cagctacgcg cggaagtttc tgatgaactc
    32941 gcaatggcgt cccgatcgct gcgcggtcat tgccggggcg ctgcgttacc cacgttatcg
    33001 ctggtacgac cgttttatga tcaagctgat tatgaagatg tcaggcggtg aaacggatac
    33061 gcgcaaagaa gttgtctata ccgattggga gcaggtggcg aatttcgccc gagaaatcgc
    33121 ccatttaacc gacaaaccga cgctgaaata agcacaaaga a
//
LOCUS       DAZGJW010000041        32916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZGJW010000041 DAZGJW010000000
VERSION     DAZGJW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.041"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(401..805)
                     /locus_tag="ACHKIK_001960"
                     /pseudo
     CDS             complement(401..805)
                     /locus_tag="ACHKIK_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspV"
     gene            complement(888..975)
                     /locus_tag="ACHKIK_001961"
     tRNA            complement(888..975)
                     /locus_tag="ACHKIK_001961"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(939..941),aa:Ser,seq:tga)
     gene            1402..2520
                     /gene="hyaA"
                     /locus_tag="ACHKIK_001962"
     CDS             1402..2520
                     /gene="hyaA"
                     /locus_tag="ACHKIK_001962"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO9083142.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            2517..4310
                     /gene="hyaB"
                     /locus_tag="ACHKIK_001963"
     CDS             2517..4310
                     /gene="hyaB"
                     /locus_tag="ACHKIK_001963"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO9083143.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            4329..5036
                     /gene="hyaC"
                     /locus_tag="ACHKIK_001964"
     CDS             4329..5036
                     /gene="hyaC"
                     /locus_tag="ACHKIK_001964"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO9083144.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWMGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            5033..5620
                     /gene="hyaD"
                     /locus_tag="ACHKIK_001965"
     CDS             5033..5620
                     /gene="hyaD"
                     /locus_tag="ACHKIK_001965"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO9083145.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            5617..6015
                     /gene="hyaE"
                     /locus_tag="ACHKIK_001966"
     CDS             5617..6015
                     /gene="hyaE"
                     /locus_tag="ACHKIK_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO9083146.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVLLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            6012..6869
                     /gene="hyaF"
                     /locus_tag="ACHKIK_001967"
     CDS             6012..6869
                     /gene="hyaF"
                     /locus_tag="ACHKIK_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO9083147.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            7003..8548
                     /gene="appC"
                     /locus_tag="ACHKIK_001968"
                     /pseudo
     CDS             7003..8548
                     /gene="appC"
                     /locus_tag="ACHKIK_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
     gene            8560..9696
                     /gene="appB"
                     /locus_tag="ACHKIK_001969"
     CDS             8560..9696
                     /gene="appB"
                     /locus_tag="ACHKIK_001969"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO9083148.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPSAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            9709..9801
                     /gene="cbdX"
                     /locus_tag="ACHKIK_001970"
     CDS             9709..9801
                     /gene="cbdX"
                     /locus_tag="ACHKIK_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO9083149.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            9881..11179
                     /gene="appA"
                     /locus_tag="ACHKIK_001971"
     CDS             9881..11179
                     /gene="appA"
                     /locus_tag="ACHKIK_001971"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO9083150.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(11294..13474)
                     /gene="etk"
                     /locus_tag="ACHKIK_001972"
     CDS             complement(11294..13474)
                     /gene="etk"
                     /locus_tag="ACHKIK_001972"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO9083151.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(13494..13940)
                     /gene="etp"
                     /locus_tag="ACHKIK_001973"
                     /gene_synonym="yccY"
     CDS             complement(13494..13940)
                     /gene="etp"
                     /locus_tag="ACHKIK_001973"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO9083152.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(13928..15067)
                     /gene="gfcE"
                     /locus_tag="ACHKIK_001974"
     CDS             complement(13928..15067)
                     /gene="gfcE"
                     /locus_tag="ACHKIK_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO9083153.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(15113..17209)
                     /gene="gfcD"
                     /locus_tag="ACHKIK_001975"
     CDS             complement(15113..17209)
                     /gene="gfcD"
                     /locus_tag="ACHKIK_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO9083154.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(17209..17955)
                     /gene="gfcC"
                     /locus_tag="ACHKIK_001976"
     CDS             complement(17209..17955)
                     /gene="gfcC"
                     /locus_tag="ACHKIK_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO9083155.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(17952..18596)
                     /gene="gfcB"
                     /locus_tag="ACHKIK_001977"
     CDS             complement(17952..18596)
                     /gene="gfcB"
                     /locus_tag="ACHKIK_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO9083156.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(18703..19008)
                     /gene="gfcA"
                     /locus_tag="ACHKIK_001978"
     CDS             complement(18703..19008)
                     /gene="gfcA"
                     /locus_tag="ACHKIK_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HFO9083157.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(19450..19662)
                     /gene="cspH"
                     /locus_tag="ACHKIK_001979"
     CDS             complement(19450..19662)
                     /gene="cspH"
                     /locus_tag="ACHKIK_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO9083158.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            19948..20160
                     /gene="cspG"
                     /locus_tag="ACHKIK_001980"
     CDS             19948..20160
                     /gene="cspG"
                     /locus_tag="ACHKIK_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO9083159.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            20171..20359
                     /gene="ymcF"
                     /locus_tag="ACHKIK_001981"
     CDS             20171..20359
                     /gene="ymcF"
                     /locus_tag="ACHKIK_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO9083160.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            20334..20564
                     /gene="ymcE"
                     /locus_tag="ACHKIK_001982"
     CDS             20334..20564
                     /gene="ymcE"
                     /locus_tag="ACHKIK_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO9083161.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            20554..20727
                     /gene="gnsA"
                     /locus_tag="ACHKIK_001983"
     CDS             20554..20727
                     /gene="gnsA"
                     /locus_tag="ACHKIK_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO9083162.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(20775..21848)
                     /gene="yccM"
                     /locus_tag="ACHKIK_001984"
     CDS             complement(20775..21848)
                     /gene="yccM"
                     /locus_tag="ACHKIK_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO9083163.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(21920..24664)
                     /gene="torS"
                     /locus_tag="ACHKIK_001985"
     CDS             complement(21920..24664)
                     /gene="torS"
                     /locus_tag="ACHKIK_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO9083164.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISTHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVADLEAWLNKKDLNAI
                     "
     gene            24747..25775
                     /gene="torT"
                     /locus_tag="ACHKIK_001986"
     CDS             24747..25775
                     /gene="torT"
                     /locus_tag="ACHKIK_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO9083165.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(25748..26440)
                     /gene="torR"
                     /locus_tag="ACHKIK_001987"
     CDS             complement(25748..26440)
                     /gene="torR"
                     /locus_tag="ACHKIK_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO9083166.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            26570..27742
                     /gene="torC"
                     /locus_tag="ACHKIK_001988"
     CDS             26570..27742
                     /gene="torC"
                     /locus_tag="ACHKIK_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO9083167.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            27742..30288
                     /gene="torA"
                     /locus_tag="ACHKIK_001989"
     CDS             27742..30288
                     /gene="torA"
                     /locus_tag="ACHKIK_001989"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO9083168.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            30285..30884
                     /gene="torD"
                     /locus_tag="ACHKIK_001990"
     CDS             30285..30884
                     /gene="torD"
                     /locus_tag="ACHKIK_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO9083169.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVGRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(30977..31282)
                     /gene="cbpM"
                     /locus_tag="ACHKIK_001991"
     CDS             complement(30977..31282)
                     /gene="cbpM"
                     /locus_tag="ACHKIK_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO9083170.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVTHP"
     gene            complement(31282..32202)
                     /gene="cbpA"
                     /locus_tag="ACHKIK_001992"
     CDS             complement(31282..32202)
                     /gene="cbpA"
                     /locus_tag="ACHKIK_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO9083171.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            32462..>32916
                     /locus_tag="ACHKIK_001993"
     CDS             32462..>32916
                     /locus_tag="ACHKIK_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083172.1"
                     /translation="MSSNIHGISCTANNYLKQAWNNIKNEHEKNQKYSITLFENTLVC
                     FMRLYKEIRRQKAEDYIPCLECDSLEKEFEEMQNDNDLSLFLRTLRTNDTETYSGVSE
                     GITYTIQYVRDIDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACD"
BASE COUNT         7860 a         8325 c         8616 g         8115 t
ORIGIN      
        1 tttgcatgaa atctaccttt tgtgaaattc tcgtcaatat tttactcaaa atgatcggct
       61 gtaggaataa actatttgat attattgcgt ttttagagtt gttttcaatc agctatagat
      121 agggcttggc gccagaatgg agaatattta acgagaaata gaagctttgt atcagacggg
      181 cttccctgaa tagagaagtt aaataatcag gatgatgcta atgaatatca ggaacaaggt
      241 gagtgtcttc atgttcactg aaacagggtg tcacaaaaaa tatggctata agatgatcaa
      301 tgtgatttga aatatttata ggtttacagg gggctgggca tgcatatctt actgcaatgc
      361 agaatatgtg ctgcttggcg cagctcttta ttgagaatgt tcaatctttt gtttcagttt
      421 cgtgaaggat acggacggtt tcttgtgtaa tattatacat cgcctgttta tctttactgc
      481 atactcctgc aagtgttaat gctactccag cataaatttc tggataacta tgtgtaaata
      541 tatccagtga gtttacatgt ttctctttca ttcctgcatc acaacgaacc ttatttatac
      601 ctgtcgccat tgattgggga gggctagagc cttctgcacg aaaatagtga tgtgacagat
      661 ctgagatagc tttgtatcct tctgcctggt tcaggcctgg agcagtccat gttttacaaa
      721 aatactcggc agcgtaacgt gttgcaagag cggcatcaga cgaggaacct gaggttccgc
      781 tcatgaccga taatctcatc cgaataaaaa tataccaatt ggtataccaa gtttatacca
      841 agagccaaaa aacaaagggg ctaccatttg gtaacccctt gattatttgg cggaagcgca
      901 gagattcgaa ctctggaacc ctttcgggtc gccggttttc aagaccggtg ccttcaaccg
      961 ctcggccaca cttccggaat gacgcgcact ataaacatcc cgatgcggcg tgtaaacccc
     1021 taatttgttt gtttgcctga aaaacagcca aaagtgcatt gatagcgtga aataacagca
     1081 gattgatcat ttcatcacca tgaattcctt ctcttttact cgtttagcaa ccggctaaac
     1141 atccccaccg cccggccaaa agaaaaatag gtccattttt atcgctaaaa gataaatcca
     1201 cacagtttgt attgttttgt gcaaaagttt cactacgctt tattaacaat actttctggc
     1261 gacgtgtgcc agtgcagaag gatgagcttt cgttttcagc atctcacgtg aagcgatggt
     1321 ttgccttgct acagggacgt cgcttgccga ccataagcgc ccggtgtcct gccggtgtcg
     1381 caaggagaag agacgtgcga tatgaataac gaggaaacat tttaccaggc catgcggcgt
     1441 cagggcgtta cccggcgcag ctttctcaaa tattgtagtc tggctgccac gtcgctggga
     1501 ttaggcgcgg gaatggcacc aaagattgcc tgggcgctgg agaacaaacc ccgcattccg
     1561 gtggtatgga tccacggtct ggaatgcacc tgctgtaccg aatcttttat ccgctccgct
     1621 cacccactgg cgaaggacgt catcctttcc ctgatttccc tcgattatga cgatactttg
     1681 atggctgccg ccggaaccca ggcggaagaa gtcttcgaag acatcatcac gcaatacaat
     1741 ggcaaatata tcctcgcagt agaaggtaat ccgccgctgg gcgagcaggg gatgttctgt
     1801 atcagcagcg gtcgaccgtt tattgagaaa ctcaaacgtg ccgctgccgg agccagcgcg
     1861 attatcgcct ggggaacctg cgcgtcctgg ggctgcgtgc aggccgcgcg gcccaatccg
     1921 acgcaggcaa cgcctatcga caaagtcatc accgacaaac ccattatcaa agtacctggc
     1981 tgcccgccga tcccggatgt gatgagcgcc atcattactt acatggtgac ctttgatcgc
     2041 ttgccagatg tcgacagaat gggccgtccg ctgatgttct atggtcagcg aatccacgat
     2101 aaatgctatc gccgcgccca cttcgacgcc ggagagttcg tccagagttg ggatgatgac
     2161 gctgcccgca aaggttactg cctgtacaaa atgggctgca aagggcctac cacctataac
     2221 gcctgttctt ccacacgctg gaatgatggc gtttctttcc caatccagtc tggtcacggc
     2281 tgcctgggct gtgcggaaaa tggtttctgg gatcgcggtt cgttctacag ccgcgtggtc
     2341 gatattccgc aaatgggtac tcattccacc gccgataccg tcggtttaac cgcgcttggc
     2401 gtggtggcag cggctgttgg tgtgcacgca gtcgccagcg ccgttgacca gcgcagacgt
     2461 cataaccagc aacctacaga aaccgaacat cagccaggca atgaggataa acaggcatga
     2521 gcactcagta cgaaactcag ggatacacca tcaataatgc cggacgccgc ctggtggtcg
     2581 acccgattac gcgcatcgaa ggccacatgc gctgcgaagt gaatattaac gatcagaatg
     2641 tgatcaccaa tgccgtctcc tgcggcacca tgtttcgcgg gctggagatc atcctacaag
     2701 ggcgcgaccc gcgcgatgcg tgggcgttcg ttgaacgtat ctgcggcgtc tgtactggcg
     2761 tacacgccct ggcttcggtt tacgccatcg aagatgctat cggtattaaa gtgccggaca
     2821 acgcaaatat catccgcaac attatgctgg caacgctctg gtgccacgat catctggtgc
     2881 acttctatca gcttgccggg atggactgga tcgatgtgtt agatgcgctg aaagccgacc
     2941 cgcggaaaac ctccgaactg gcgcaaagtc tctcttcttg gccgaaatca tcccctggct
     3001 atttcttcga cgtacaaaac cgcctgaaaa aatttgttga aggcgggcag ttggggatct
     3061 tccgcaatgg ctactggggg cacccgcagt acaaactgcc gccagaagct aacctgatgg
     3121 gctttgccca ctatctcgaa gctctcgatt tccagcgtga aattgtcaaa atccacgcgg
     3181 tctttggcgg taaaaacccg catccaaact ggattgtcgg cgggatgcct tgcgccatca
     3241 acattgacga aagcggcgcg gtcggggcag tcaatatgga acgcctgaac ctggtgcagt
     3301 caattatcac ccgcacggcg gacttcatta acaacgtgat gatccccgac gccttagcca
     3361 tcggtcagtt caacaaaccg tggagcgaaa tcggcactgg tctttctgat aaatgcgttc
     3421 tcagctacgg cgcattcccg gatattgcca acgactttgg cgagaaaagt ctgctgatgc
     3481 ctggcggcgc ggtgattaac ggcgacttca acaatgtgct gccagtggat ttggttgatc
     3541 cgcagcaggt gcaggagttt gtcgaccacg cctggtatcg atatcccaac gatcaggtcg
     3601 ggcgtcatcc gttcgatggc atcaccgacc cgtggtacaa ccccggcgat gtcaaaggca
     3661 gcgataccaa cattcagcag ctgaatgaac aggaacgcta ctcgtggatc aaagcgccac
     3721 gctggcgcgg taacgcgatg gaagtggggc cgctggcgcg cacgttaatc gcttatcaca
     3781 aaggcgatgc tgcgaccgtt gagtcggtcg atcgcatgat gtcggcgttg aacctgccgc
     3841 tttccggtat ccagtcaacg ttaggccgca ttttgtgccg cgcgcacgaa gcgcagtggg
     3901 ccgcaggtaa gttgcagtat ttcttcgaca agctgatgac caacctgaaa aacggcaatc
     3961 tcgccactgc ttccacggaa aaatgggaac ctgcaacctg gccgacagag tgccgtggtg
     4021 tcggttttac cgaagcgccg cgcggggcgt taggccactg ggccgccatt cgcgatggca
     4081 agattgatct ctaccagtgc gtggtgccga ccacctggaa cgccagcccg cgcgatccca
     4141 aagggcagat tggcgcttat gaagcggcgc tgatgaacac caaaatggcg atccccgagc
     4201 aaccgctgga gatcctgcgt actctgcaca gctttgaccc gtgcctcgcc tgttcaacac
     4261 acgtgctggg cgacgacggt agcgagctga tctctgtgca ggtgcgttaa cagcgaagga
     4321 gaatcatcat gcaacagaaa agcgacaacg ttgtcagcca ctatgtcttt gaagcgccag
     4381 tccgcatctg gcactggttg acggtgttat gcatggcggt gttgatggtc accgggtact
     4441 ttatcggcaa gccgctacct tccgtcagcg gcgaggcgac gtatctgttc tatatgggct
     4501 acatcaggtt aattcacttc agcgccggga tggtttttac cgtggttttg ctgatgcgga
     4561 tctactgggc ttttgttggc aatcgatact cccgcgagct gtttatcgtg ccggtatggc
     4621 gtaaaagctg gtggcagggc gtgtggtatg aaatccgctg gtatctgttt ctggcaaaac
     4681 gtccgagtgc cgatataggc cataatccca tcgcccaggc ggcgatgttc ggctatttcc
     4741 tgatgtcggt ctttatgatc atcactggtt ttgcgctgta tagcgaacac agccagtacg
     4801 ctatttttgc gccgttccgt tatgtggtgg aatttttcta ctggatgggt ggcaactcaa
     4861 tggacattca cagctggcat cggctgggga tgtggctgat tggcgcgttt gtgatcggtc
     4921 atgtctacat ggcgctgcgt gaagacatca tgtccgacga cacggtgatc tccaccatgg
     4981 tcaacggcta ccgtagccac aaatttggca aaataagtaa caaggagcgt tcatgagcga
     5041 gcaacgcgtg gtggtcatgg ggctgggcaa cctgctgtgg gccgatgaag gcttcggcgt
     5101 gcgggtggcg gaacggctgt atgcccatta ccactggccc gagtatgtgg agattgtcga
     5161 tggcggtact cagggactga acttgctggg gtatgtcgaa agcgccagcc atctgttgat
     5221 tctcgatgcc attgactacg ggctggaacc tggaacgctg cgaacctatg ccggagaacg
     5281 cattccggct tatctcagcg cgaagaaaat gagcctgcat cagaacagtt tctccgaagt
     5341 gttggcgctg gcggatatcc gcggacatct gccagcacat attgccctcg tcggtctgca
     5401 acccgcaatg ctcgacgact acggcggtag cctgagcgaa ctggcacggg agcaactgcc
     5461 cgctgcggaa caggcggcgc tggcgcagct ggctgcgtgg ggaattgtgc cgcaaccggc
     5521 taatgaatcg cgctgtctca attatgactg tctgtcgatg gaaaattacg aaggcgttcg
     5581 cttgcgccag taccggatga cacaggagga gcagggatga gcaacgacac gccatttgat
     5641 gcgttgtggc aacgaatgct ggcgcgcggc tggacgccag tcagtgaatc ccgtcttgac
     5701 gactggctta cgcaagcgcc agacggcgtg ctgttattaa gcagtgaccc gaaacgcacg
     5761 ccagaggtca gcgataatcc ggtaatgatt ggcgaattac tgcgcgagtt tcccgactat
     5821 acatggcagg tggcgattgc tgaccttgag cagagcgaag ccatcggcga tcgctttggc
     5881 gtctttcgct ttcctgccac tttagtgttt accggcggaa actatcgcgg cgtgctgaat
     5941 ggtattcacc cgtgggcgga actgataaac ctgatgcgcg ggcttgtcga accgcagcag
     6001 gagcgtgcct catgagcgaa acatttttcc atctgctggg gccaggaacg caaccgaacg
     6061 atgacagttt cagcatgaat ccactgccga tcacctgtca ggtgaatgat gaaccgagta
     6121 tggcggccct ggagcaatgt gctcacagcc cgcaggtgat tgcgctgtta aacgagttac
     6181 aacatcaact aagcgaacgc caaccgccgt tgggcgaggt gctggcagtc gatctgttaa
     6241 atctcaacgc cgacgatcgt cactttatca atacgcttct cggggaaggg gaagtgtcag
     6301 tgcgcattca gcaggctgac gacagtgaaa gtgaaataca ggaggcgatc ttctgcggat
     6361 tatggcgggt gcgcagacgt cgcggcgaaa agttgctgga ggacaaactg gaggctggct
     6421 gcgcgccgct ggcgttgtgg caggcggcaa cgcaaaatct cttgccgaca gattcgctgt
     6481 taccgccgcc cattgatggc ctgatgaatg gcctaccgtt ggcgcatgag ttactggcac
     6541 atgtacgtaa ccccgacgcg cagccgcaca gcattaatct gacgcaatta cccatcagcg
     6601 aggctgatcg gctttttctc tcacgtctct gtgggccggg aaatattcag attcgtacca
     6661 ttggctatgg cgagagctat atcaacgcca cggggttacg ccatgtctgg catttacgct
     6721 gtacggacac cttaaaaggc ccgttactgg aaagttatga aatctgccca ataccggaag
     6781 tggtgctggc agcgccagaa gatttggtcg actctgcgca gcggcttagc gaggtatgtc
     6841 agtggctggc ggaagctgca ccgacgtaaa aagacggtaa gtatcgcttt cagtcttatg
     6901 aatatcgcaa tcggcgaata cctctggtcg tagagtttca ggataaagag ggagatctac
     6961 cattatcggg ttatttttct ctcttcgcct acaggagtgc gcatgtggga tgtcattgat
     7021 ttatcgcgct ggcagtttgc tctgacctgc gctgtatcac tttttatttg taccccttac
     7081 cctggggctg atttttttgc tggctatcat ggaaaccatt tacgtggtca ccggcaaaac
     7141 aatctaccgc gatatgacgc gcttctgggg taagctcttc ggtatcaatt ttgctcttgg
     7201 cgtggctacc ggcctgacca tggagtttca gtttggtact aactggtcat tctattccaa
     7261 ctatgtgggc gatatttttg gcgcaccact ggcgatggaa gcattaatgg ccttcttcct
     7321 cgaatccacc tttgtcgggc tgttcttctt cggctggcaa cggctgaata aataccagca
     7381 cctgctggtg acgtggctgg tggcgttcgg ttcaaatctc tctgcgttgt ggatattgaa
     7441 tgccaacggt tggatgcaat acccgaccgg tgcgcatttt gatatcgaca ccctgcgtat
     7501 ggagatgacc agcttcagcg agctggtctt taacccggtc agccaggtga aatttgtgca
     7561 caccgtaatg gcgggctacg tgaccggggc catgtttatt atggcgatca gcgcctggta
     7621 tttactgcgc ggacgggagc gcgatgtcgc attacgttcg tttgccatcg gttccgtctt
     7681 cggtactctg gcgattatcg gtaccctgca actcggagac agttctgcgt atgaagtcgc
     7741 gcaagtacaa ccggtaaaac tggcggcgat ggaaggggag tggcaaacgg aacctgcacc
     7801 tgcaccgttc catgtggttg cctggccgga acaggatcaa gagcgtaacg cctttgccat
     7861 caaaattccc gcgctgctag ggatcctcgc cactcactca ttagataaac ccgtgccggg
     7921 tctgaagaat ttgatggctg aaacctaccc acgcttgcaa cgcggacgta tggcctggct
     7981 gttaatgcag gaaatatcgc aaggcaatcg tgagccgcat gtgttgcagg cattccggga
     8041 actggaaggt gacctgggct acggcatgtt gctctcccgc tatgcgccgg atatgaatca
     8101 tgtcacagcc gcacagtacc aggcggcgat gcgtggcgcg atacctcagg ttgcgccggt
     8161 attctggagt ttccgcatca tggtgggctg tggttccctg ctgctactgg tgatgctgat
     8221 tgcgcttgtc cagacgctgc gtggcaaaat cgaccagcat cgctgggtgc tgaaaatggc
     8281 gctctggagt ttgccgttgc cgtggattgc gattgaagcc gggtggttta tgaccgagtt
     8341 tggtcgtcag ccgtgggcga tacaggacat cttaccgaca tactccgcgc actccgcttt
     8401 aaccacagga caactggctt tctcactgat catgatcgta gggctttaca ccctgttctt
     8461 aatcgccgaa gtctacctga tgcagaaata tgcccgtctg gggccgagcg cgatgcagag
     8521 tgaacaaccg acgcagcaac aggggtaaag gagaaaatca tgtttgatta tgaaacattg
     8581 cgcttcatct ggtggctgct gattggcgtg atcctggtgg tctttatgat ctccgacgga
     8641 tttgacatgg ggatcggctg tctgctgccg ctggtggcgc gtaatgatga tgaacgccgg
     8701 atagtgataa acagcgttgg tgcacactgg gaaggcaacc aggtctggtt gatcctcgct
     8761 ggtggggcat tatttgccgc ctggcccaga gtgtatgcag cggcgttttc cggcttttat
     8821 gtggcgatga tcctggtgct gtgctcactg ttcttccgcc cgctggcctt tgattatcgc
     8881 ggaaaaatcg ccgatgcacg ctggcgtaaa atgtgggacg ccggtctggt catcggcagt
     8941 ctggtgccgc cggtagtctt cggtatcgcc ttcggcaact tgttgctcgg cgtgccgttt
     9001 gccttcacac cgcaattacg cgtggagtat ctcggcagct tctggcaact gctgacgcca
     9061 ttccctttat tgtgcggatt gctcagcctt gggatggtga ttttgcaagg tggcgtctgg
     9121 ttacaactga aaactgttgg tgtgattcat ctgcgttcac agctggccac caaacgcgct
     9181 gcactgttgg tgatgctgtg ctttttgctg gcgggttact ggctgtgggt cggtattgat
     9241 ggctttgtac tgctcgccca ggatgctaac ggtccttcca atccgttaat gaaactggtg
     9301 gcagtgctac ctagtgcctg gatgaataat ttcgtcgaat cgcccgtttt gtggatcttc
     9361 ccgctgctgg ggttcttctg cccattgctg acggtgatgg cgatttatcg tggtcgcccg
     9421 ggttggggat ttttaatggc gtcattgatg caatttggcg tgattttcac ggcaggcatc
     9481 acgctgttcc cctttgtcat gccgtcaagc gtgagtccaa tctccagcct gacgttgtgg
     9541 gacagcactt ccagtcagct gacgctgagc attatgttgg taatcgtgct gatatttttg
     9601 cccattgtgt tgctctacac tctctggagc tactacaaaa tgtggggacg catgacaaca
     9661 gaaactctcc gccgtaacga aaacgagttg tactaaggag caggaacaat gtggtattta
     9721 ctttggttcg tcggcatttt gttgatgtgt tcgctctcca cccttgtgtt ggtatggctg
     9781 gacccgcgtc tgaaaagtta acgaacgtag gcctgatgcg gcgcattagc atcgcatcag
     9841 gcaatcaata atgtcagata tgaaaagcgg aaacatatcg atgaaagcga tcttaatccc
     9901 atttttatct cttctgattc cgttaacccc gcaatctgca ttcgctcaga gtgagccgga
     9961 gctgaagctg gaaagtgtgg tgattgtcag tcgtcatggt gtgcgtgctc caaccaaggc
    10021 cacgcaactg atgcaggatg tcaccccaga cgcatggcca acctggccgg taaaactggg
    10081 ttggctgaca ccgcgcggtg gtgagctaat cgcctatctc ggacattacc aacgccagcg
    10141 tctggtagcc gacggattgc tggcgaaaaa gggctgcccg cagtctggtc aggtcgcgat
    10201 tattgctgat gtcgacgagc gtacccgtaa aacaggcgaa gccttcgccg ccgggctggc
    10261 acctgactgt gcaataaccg tacataccca ggcagatacg tccagtcccg atccgttatt
    10321 taatcctcta aaaactggcg tttgccaact ggataatgcg aacgtgactg acgcgatcct
    10381 cagcagggca ggagggtcaa ttgctgactt taccgggcat cggcaaacgg cgtttcgcga
    10441 actggaacgg gtgcttaatt ttccgcaatc aaacttgtgc cttaaacgtg agaaacagga
    10501 cgaaagctgt tcattaacgc aggcattacc atcggaactc aaggtgagcg ccgacaatgt
    10561 ctcattaacc ggtgcggtaa gcctcgcatc aatgctgacg gagatatttc tcctgcaaca
    10621 agcacaggga atgccggagc cggggtgggg aaggatcacc gattcacacc agtggaacac
    10681 cttgctaagt ttgcataacg cgcaatttta tttgctacaa cgcacgccag aggttgcccg
    10741 cagccgcgcc accccgttat tagatttgat caagacagcg ttgacgcccc atccaccgca
    10801 aaaacaggcg tatggtgtga cattacccac ttcagtgctg tttatcgccg gacacgatac
    10861 taatctggca aatctcggcg gcgcactgga gctcaactgg acgcttcccg gtcagccgga
    10921 taacacgccg ccaggtggtg aactggtgtt tgaacgctgg cgtcggctaa gcgataacag
    10981 ccagtggatt caggtttcgc tggtcttcca gactttacag cagatgcgtg ataaaacgcc
    11041 gctgtcatta aatacgccgc ccggagaggt gaaactgacc ctggcaggat gtgaagagcg
    11101 aaatgcgcag ggcatgtgtt cgttggcagg ttttacgcaa atcgtgaatg aagcacgcat
    11161 accggcgtgc agtttgtaat gcataaaaaa gagcattcag ttacctgaat gctctgaggc
    11221 tgatgacaaa cgaagaactg tctaatgcgt agaccggaaa aggcgttcac gccgcatccg
    11281 gccactttca gttttactct ttctcggagt aactataacc gtaatagtta tagccgtaac
    11341 tgtaagcggt gctggcgcgt ttaatcacac cattgaggat agcgccttta atattgacgc
    11401 ctgcctgttc cagacgctgc attgacaaac tcacctcttt ggcggtgttc aagccaaaac
    11461 gcgcaaccag caggctggtg ccaacagaac gccccacgac cgcggcatca ctcaccgcca
    11521 gcatcggcgg cgtatcgaca atcaccagat cgtaatggtc gttcgcccat tccagtaatt
    11581 gacgcatccg atcgcgcatc agcagttcag acgggttagg tggcacctga ccgcgagtaa
    11641 tcacatcaaa gcctcctttg ccaaaatgct ggatcacttt gttgagctca tctttacctg
    11701 ccagatattc cgacaagcca tgttcattac tcacggtaaa caggttatgc gaataaccac
    11761 ggcgtaagtc ggcatcaata aataacactt tttgatcgga ctgggcgatc accgctgcca
    11821 gagttgaact gacaaacgtt ttaccactgt ctggcgtcgc accggtgatc atcagaatgt
    11881 tattctccgt ctccatcata gcgaaatgca gactggttcg tagcgcacgt acggcttcca
    11941 cagcagaatc cgccgggtta tccaccgcca ggaaggggat atttttagta cgatggcgct
    12001 gctgattaga aaataaattt ttcttacgca gacgggtgcg tttatccagc cactcggaca
    12061 ttgggatagt ggcataaacg ctgatgccgt gctcttccag ttgttccggg gcttctacac
    12121 cacgacgcaa catcgcacgc gccagcacgg cacccacaga aataaacagg ccaagaataa
    12181 aaccaagcac cacattcaac gcttttttcg gtttcactgg ctgcggctga gtgactgccg
    12241 ggtcgataat ccgcacgtta ccaatggcac tggatttcga aatactcaac tcctgctggc
    12301 ggttaagtaa ttgcagatat accgcacggc ccgcttctac gtcacgactt aaacgcaaca
    12361 cttcctgttg ggtggaaggc attgccgata cccgcttatt caggcgtttg cgttcttgct
    12421 ccagcgtctg gcgtttttcc agcagcgcac gataagttgg gtgatctttc ttatacagct
    12481 gggagatctc tgcctcgcgg aaggtcagct cattgagttg attatcaacg ttcacaatct
    12541 gctcaagaac ggctttggct tccaggttaa ggtcaaccga atcgcgctgc tggcgataaa
    12601 cgttgagttt ttcttccgcc tggtccagct cgctgcgcac ttcaggtaac tggcgctgta
    12661 agaattcaag gctttgtgaa tcctgcgccg cctggcgagc gatattctgt tgcaaatagt
    12721 tgttagcgat gctgttcaga atacgagtaa tcaactgggg atcatcacca gtcatggtaa
    12781 gttccagcat cccgctttct ttactgcgtt cgctaacggt aaaggtttcc tgcaatgcgt
    12841 taatcgcttc cagttcggta cgctggctca ggacaaactg tgttcctggt ttggccttaa
    12901 tgtccgcgat agtcagcgca acgccatctt tttccagacg ctggccgacc ataccattga
    12961 cggtgaactc ttcaccttcc agtgtatagt ggccgttttc cccaaccgtg agtgtcagtt
    13021 gctgatcctg accattcagt tgtggaatat gcatccagct gatcgccagc tcacctggtt
    13081 tttctttggt taatctcgcc cagccgcgac ccacaatcgg aaaatacttc tgctcaacta
    13141 tgtcgcgcag attcagttca gcaatggttt taccgagaat catgcgcgat tgcagcagtt
    13201 ggatctccgg tgcagactcg ggcgatgagt tagggatcat atcgctcagg ccgctgagaa
    13261 tggcgttgcc ctgtttttgc tcaacctgga ccagagtatc tgcctgataa attggtgtgc
    13321 ttaacagcga gtaagcgaca gcgatcagcg tgaataacgc ggtcacgctg ataataaact
    13381 tacggtgatc ccataactcg ccgaccagac gaagcagatc gatctcattt tcctgagtgc
    13441 tgcctggtgg cgtattcata tttttagttg tcatcgtatt ttataaactc aaattaccgg
    13501 ctgaggcgct tcgcccattc ctgactggcg cgctccaaca taccgtagac atgttcaaat
    13561 gcgtcctgac ttttacgata gggatccggg atctctttct gttccagcca ttgcccaaac
    13621 agcattgtct tcccgcggac ctcgggagca attgcggtaa cctgagcaat atgttccgac
    13681 tccattgcca gaatcagatc gtaatttcgt gccatctcag cagtgagctt acgtccggca
    13741 tgtccttcaa gagatacgcc atgattagcg gcaacatctg ccgctgtcgc atctgcaggg
    13801 tgttttacca gaccatgaac acctgccgat ttgactttta cgcccggcag acgtttacgc
    13861 agcaagcgct cgccaatggg cgaacggcaa atgttgccgg tacaaaccac caggattgag
    13921 ttaaatttta gttgggccat gtttgaatcc accgtgaagt ctctgtcagg tcatgcaccc
    13981 cggaaatcgt cggtaccagc agagaaatca cacggttcca acgagccagc ggcgcggtag
    14041 tgacataaac aatgtcgtaa ggttcaagct ggaattccgt tccgagtatc attgccgatg
    14101 cgtccttcgc attcagctga taaatattgg cgattttgcc attctgcttg ttttgcgttg
    14161 cacgaataac aaaaatcccc gtcgcatcag cgacatcctg attcataccc tcagcgttac
    14221 ccagcgcttc cgccagggtc ataccgctgc ggtccatttt cagcgtgctc tgtttaccca
    14281 cttcacccat cacgaagact ttcagatcat cgttgcgcgg aataaataaa atatcgcccg
    14341 ggtgcagcag tttgttctgg cgcaagtcgc cacgctgcat cagcgcatag aggttcactt
    14401 tggtctttac gccattctgg gtgagcacca cgttgcgcca gtctgcatct gcggtcagac
    14461 cgcccgcggc gttaatcgca tccatgatgg tcagaggaat attggtaatc ggctgctgac
    14521 ccgatttgct gacttcgccg gtgacgtacg ctttttgcga gcggaacgcc gcgacgctga
    14581 catcgacttg cgggctttca atgactgaat ccagacgcgc tgtaatctcg ttgcgtacct
    14641 gggtaagtgt tttcccggcc actttcaggc gaccaatata ggggtagaaa atcgcaccat
    14701 cggcgttaac ccagttgccg gtatcactgg cgctacggta ttgccctgcg ggggtggtta
    14761 gttccgggtg atcccagacc gtaaccatca acacatcccc aatgccgata cgatattcat
    14821 agtttgcgat ttgctgctcc agctccgggt tagcctgcga cataatcggt ttggcgcgca
    14881 actgatcaat caatcctggc gtgacgggat agatattgac cattttgtcc aggtcatact
    14941 gattgtcagg tagttcaatg acatccttgt tactggtcga taaattctga ccaggaacaa
    15001 gggtacatgc tgtcaaagac agaacagcca atgtcagtac cgaaaactta aaaatgttct
    15061 ttttcattgg tttatattta tcagtcaatg taatgttatg aattacgtca ggttattgtc
    15121 caaccggtac ttctcgctct gccgtcattg gatagagctg gtatttgcgt gacagcattt
    15181 gtccaccatc acgcgtcaat ggtgtccacg aaaccaccgc gcggttgcgg ttaggtccaa
    15241 tggtcatcaa gtcgaatgga atcgagatat aaaaaccttt actaaagccg ccttcgccgt
    15301 agtcatcttt agatacgtta ctgattgccg cccatacccc taccgccacg ccgctgtcaa
    15361 agcgtttggc gacgtcgatc gttgcgcctt tatcttttgc cagatattgc ccaacgctaa
    15421 gtttcatcag tacgccattg agcgtcggcg ggttccagta agccgtcacg aaacctgttg
    15481 gcgtggaata atcggtgaaa cgcatcatgt tgtcccagtc acgttgcttc acgtagttaa
    15541 cgtccacacc cagcgcccag ctggcatcta gcgggcgata aagcagctcg gaaccgacac
    15601 cggcgtacat cgtttccaga taaccgccat acacctggcc atagaaacca ttgcctaagt
    15661 cggcaaagta gttcgcctgc aagttgttga gataaacgtc attgcgaacg taatcacgga
    15721 tatgcgtgcg cacgcggggc agggtagagt ccgcgggcaa cagcgaagac ttaaacttgt
    15781 cgtagttgtt gtaaatattg gtgaaaatac cgccatcaag cagcaggtgg tcggtaaacc
    15841 agtagcgggc actggacatc agccccagct ggaacatata gaaatcttcc gggccgccca
    15901 gcgactgtga aagtgttggg ttgaaagagt agctaaagcg atcttcacga atacggtagc
    15961 cccgaccaaa ggcagaaaga tcttctgctt caacacgttg ttgttgaagt tgctctgatt
    16021 gaccaggcgc tgtaccagcc agctgtttgc gcaggctggc tacgtcggtt tcggtagtca
    16081 ccatcgccat atgctcgcgc ttttgcgtca cgctaatctt ctcaacgcct tgcggcaggt
    16141 tattcaccag aatccggttg gcacgatcga ccgcttcgcg agaatcacgg tatttgtatt
    16201 gttgaccaga catatacagc gtcttatcgc gcagctgaat ttccggtgct tcaaaacctg
    16261 cgttgtactt cagcgcggta agttggttcg ctactgtggt gtactgcaat ccttcagatt
    16321 caggcgcagg ttgatatgcc ggttttggcg tatcgcgcag ggcagggcgc agatcgttga
    16381 aattggtccg taacgtgaag ccaaacatca acgtgttacc gcgttcataa ctcaggttga
    16441 gatctgccca gctggcagcg cgataaactg cgccgacgtt gaaatggctt gcctgaggca
    16501 gtttgccagc gaaatcattc tggtaattgt tgccatcgta ttcgagtttc agacgcaggg
    16561 gattccacgg cgtttgatac tcaatgccgc caaagatgga agccgggcca cgaaagatat
    16621 cgctaaagct gatatcgccc gcatcgtgag actctgcgcg atgacaatat ttatcgctca
    16681 cccggcaaaa cgggttggta atattgcccg cattaccggc gtagccccat gccatcccga
    16741 gggtgaaatc aaatggcccc gcttgcttgc tggccaccag atactcaccg tcaaacagac
    16801 ccgtaccagc aatatcacgt ttaccaaacg ccacttgcgg tagccagtaa ccttcttccc
    16861 acaggcgaag tttaaaatcg aatgatttgt ctttgtatga ctgatcgccg ctgaaatctt
    16921 cccactggct atatttgcgt gtgcgcacat ccgtataacg aatggtgcct tccagccatg
    16981 ggaacagcgc cacggaggtg gagtagaacc ggtattgatc gttatcccgg tagttgacgc
    17041 tgaattcacc ttccggtgcg atgcgcgcat ttggcatctg caacaatcct gtgccgccaa
    17101 agtctgattg cgacgaaccc agcggatccg ggtaggttaa tacttcagca tgacaggcac
    17161 tggagacggc aatggccagg cagcttaaaa gataagaatt cttcttcatc agtcaggaac
    17221 acgttgcgtt aaaacagaca caatctggtc atttaaatca gcatattttt cgggcagtac
    17281 atgcgcagaa aagcccaacc aaagctggct gccaggcggt ggttccacat gtcttttatt
    17341 ccacagggca acaggggcga cgacagtctc accctcaggc gtaatcacca taacgttatt
    17401 cttgtccgcg cctgcaaggc gggggtgatc ttgcagataa tcggtaacgc tacggcctgc
    17461 ctgccagggc aattgcccgg ccccagaaac cgcacccagc agagtaatag tcaccggacg
    17521 ctgtacggta tagagcgtat agtccccgac caacggggga ttgctgttct catccactcg
    17581 tacaaaatca gggtccagtt tgaccggtaa gcgaccagtg atattgagat tgagtaactg
    17641 ctggcgcacg gatttaatcg ttgccgccac atcgtcgtca gcttctgctt cccaggaagc
    17701 aagctgtgcc atgacatgtt ggtagtcttt aagcgcttta gctttcgccg cagagtcggt
    17761 cagcaaggca ccaggccacc agaggcgatc gcgcagttgc ggttgtgtca ccaactggac
    17821 aacattttcc atggggccaa cggaaagtgt ctgttgttcg ccaggcagat aaatagtcac
    17881 cgttccttgc gcaaaggcat ggggtgtcat tacgtaaagt acgctggcaa tgaaatacga
    17941 ctgtaattta ttcatggtgc gggtttaaga aacgtcattt ctacgggaat aacccctgcg
    18001 cctaacattt gccgactttg acgaacctgg ccagtagccg aatcgaccca gaatgtgttt
    18061 tgccactgtt tttctggccg cgtggaagtc acctcttcct gccagacatt acacagcaca
    18121 ttgcgacccg caatattcag cgtctctaat ccagcaaagg aaaaagtaga actcagggtc
    18181 gcagagcgaa aatggttgtc ttcactccag agaatatcgc gcgtccagcg gctgccatct
    18241 tttattgcca gcgcgttacg cagagggtcc tgcccggaat ttgtcacttc cagcagatta
    18301 ttattaagtt tgacggtttt taaaagacgt ccattgtggg taaccagcat ggcgttatcc
    18361 tgggataacc atttgctttg ttcttgttct atatatccca gtaccacaaa gattcgttgc
    18421 ccttcattaa ggcgtaaata catcgtggaa taaggcaacg cctggatctg ctgatccgct
    18481 acggtgatat cctgattatc gaaaaggctg gcgcgaaatg tatcgaccat actttgctgg
    18541 ctgtgcgtac atcccgcaag aaatagtgcg aaaatcgata aaataagagg gcgcaccttt
    18601 aatccttgaa aggttaatta aaaaaatgca aatctctaaa gcccggtacg aatatcgcaa
    18661 ccgggtatca tttaacttta atttcgacgc gttaaagcag tattagcgtg tagtggacgt
    18721 ggtggttgag gtgttatgag aaccgccatc gccactgtca tttgctgcaa caactgccaa
    18781 cgtcgccaca ccaacagccg ttgccacacc gacaactgtc gccgtgccga cagcgccagc
    18841 agccacgcca ccagtggtag tagcagtcgt ggttgcgccg gtagtggtcg ttgtggtgcc
    18901 agtagtaccc gtcgttgcct cggtcccgtt tgttgcttca ggggcagcaa acgcagctgg
    18961 cgtcgtcaac atgaaggcca taaggattgc agaaagcttg tgtttcataa cttttccttt
    19021 attcatcgca tggacaatac gtacgcttga gatcaggatt gtctactttg agtataacat
    19081 ccttgtgaaa tctccctttc ctcataccgc ttgcactata tactgcgggt aggaaaggcg
    19141 cgcagaggga aatataagat tgttaactat ccccctctgg aaatacgact tgtgagcata
    19201 agataaatct tagcaaaata caaattaaag ataaggaaat tgttacgaaa gctattaatt
    19261 attgttggta atatagtttc aagtggtact atagctggct gttaaatggc atttataact
    19321 attaggtgca gagatattcg cttaaatgga ttagtttctt tgtggaaaag taactggtgt
    19381 tataatgtat gatgttgact attgcactat tccaataaaa ccagtcagct ttaaacaagc
    19441 agcgtcatat taagagagat aaacatttgc cgctgttggt cctcgcaggc catttacgcg
    19501 gcaaaattcc acacgtaatc ctggtataag cacttctgcg tcgcggggag tgaatgcgga
    19561 aatatggacc tgaacttctt tacgaccgtc ggaggggata atgaatcctt tgccgctttt
    19621 gcgatcaaag gttttgacaa ttcctgtcat tttacgggac aaacaaattc cttactgaaa
    19681 atactgcgct gcactatacg gggttaataa aataaagcca gcgatattta agaccgccgg
    19741 acggctaaaa taaaatttgc ttaatctcaa ttatcatgcg ttaatagctg cgtcggtttg
    19801 aaagacagac agcatacaaa gtagtttact aaagcagttc tcattatcag gcattatccc
    19861 cttcttttga gtctctctcc tgaacactaa gtagtttctg tattaaagcc ctgtttgccg
    19921 aaaggcccaa aatgaaggaa gtaaaatatg tctaataaaa tgactggttt agtaaaatgg
    19981 tttaacgcag ataaaggttt tggctttatc actcctgatg atggcagcaa agacgttttc
    20041 gtccatttca ctgccatcca gagcaatgaa ttccgcacgc tgaacgaaaa tcagaaagtt
    20101 gaattttcta ttgagcaggg gcaacgtggc cccgcggcag cgaacgttgt tacgctctaa
    20161 ggttgccgtt attactcaac atctccattt ccgctgtcca tgttgtcatg gttcacagta
    20221 ccgtacatcg gcattcgatg tgacggagcg aaaccctttg ggcgctaagt gtattttttg
    20281 taaatcgacg atgatcacct ttgataacgt cgcgctgcaa atacgcacgg accatgcgcc
    20341 gctggatttc acaaaataat atcaggctcc ctcgtggagc cttttttata tctgccttat
    20401 ttttcttcaa cgctgtatgt atagtaagcg ataacctgtt gattattgaa tctttcgggg
    20461 agatggctta taacatttct tacctgacca gggtaccggg aaccaacacc ttactggcgt
    20521 gttgctgtct ttcaagacca gaagaggtta acagtgaata ttgaagagtt aaaaaaacaa
    20581 gccgaaacgg aaatcgccga ctttatcgcg caaaaaatcg ccgagctgaa caagaataca
    20641 gggaaagaag tctctgaaat tcgcttcacc gcacgagaaa aaatgaccgg gcttgaaagt
    20701 tatgatgtca aaatcaaaat aatgtgattt tgtgaacatc acccgtgcga ggtgatgttc
    20761 cgcttgttgc taatttagtg accaatcatt ggcgcttgtg gaattaagcg tcggtacaat
    20821 tcatccggta ccgggctttg ccatactccc gcatacattg cgtaaccaat caccgcaaac
    20881 ataatcccca gaaccagtag cgtcattaac cagccagaca acgcaaaggc ttttttattt
    20941 gccgcaggtt tttgcagtga aaaggtcaat gttgaggcta ccggacatga ctctacgcaa
    21001 gtcatacagc cggtacattc cactgttcgt acctgaatta atttatcgac cgggatccgt
    21061 gatgggcaat tttttgcgca tttgccacag tcgatacaac tttcggcatt gcgacgaatc
    21121 ttaaacggcg acaatagcga aaccacgccc atcagcgcgc catatgggca aagataacga
    21181 caccaggcat ggcgaataaa caggctggca atcagcaaaa cggtcacgct gattaatgtc
    21241 gcggtcccca tatgacgaaa gaaatcgagc attttaacgt ccatcaccac gctgtagggc
    21301 gacaacataa aatagtgaat cgcctgagcg ggcatcaata atgcgatata gagaaaaaaa
    21361 ctcaacagca aatacttcac gccgcgcaga ggaatatcca gccagcgggg aaggacacat
    21421 tgccgaccaa acagtttgtt accgagatcg ccgattaatt cagaaagcgt accaaccggg
    21481 cataaccatg agcaaaaggc ctttttgagt aatagactga tgacgataaa agcgaccaat
    21541 aacagcatcg cggcggcgtg gacggacggt aactgacctg ttgtaaggct atatttcaga
    21601 ttcatcagac cggcaatcgg tagccagcct tcgatacctc ccggtctggc gacaaatgtc
    21661 gtgctacttg ccgtttcgta atagcgcacc caataccaga acgtgatggc aatataaata
    21721 ttcattgcca acagtaataa ttgcgtcgct ttacgccagg tcgtggcatt gcgccagtca
    21781 ttccacggta atttgccgcc cgtcgtgcct ggccgccgct gccagcgggt tctttttttc
    21841 tctgccatga ttttgccagt ccgttaagtt gcataccaaa tgccactatt ctagttgttc
    21901 ttaactggct gatattgatt caaatcgcgt tcaggtcttt cttattcaac catgcttcca
    21961 gatcggcaac actgcgtgta atttcttcgt gtggaagggg ggcagataat ggctgctgct
    22021 ccagttgtgc gcatagctgg ctggcgatac gcattcccaa actcgagcaa ctgcttttta
    22081 gctgatgcgc ggcacgcttt attttctcgc tatcctgact ggcgcgggca atgtcgattt
    22141 catcgagaag cggcagggca tgttgtgtaa ataataccag ccattcgtgg atcttctccg
    22201 tccccattaa ctgagcatct tcattgagtt gcgatacatc cagcgattga tcgttattga
    22261 cttgcagttg gagatagtgc gccagtaact gaccgagcac ttcacgcggc accggtttag
    22321 ggataatccc gcggaataat gaactggtac gctggcgcag cgtttcgtca atgacatggg
    22381 cgctaaagcc aatcaaaacc agcgacggat attgctgtgc cagttgtcgg gcaagcgtaa
    22441 tgccgtcgac atccggcaga tcaaaatcca ccagtgcggc agcaaacggt tcgctatttt
    22501 gcagtgtctc taaagcctgc gcggcattgc caacagcaac aacctgcgca ccactggtgt
    22561 tcagcatctc aacggtaatt cgctgggtta gcgggttatc ttcaattaac agcaaacgta
    22621 aaccgtcaag acgcaccgcc tgattgactg tttttggcac gggtgccgtg gcaacacgta
    22681 acggcaagcg taaacaaaaa cagcttccaa cctccggcgt gctggtggcg ctcagttcgc
    22741 cgcccatcgc ctgggccaga cggctactga tagtcagccc cagcccggtg ccgccgcgtt
    22801 tgccgcttac ctgcacaaat ggctggaaga tttctgccag tttcgcggga tcaataccgc
    22861 agccgctgtc ttccacttcg accagccatt gctcgccatc agtgcgacta cgcaggataa
    22921 tgtacccttc gtcagtaaaa cgcagggcgt tgctcaacag gttggttata acctgacgaa
    22981 tacgtcgtgg atcgcccatt aacgcgcacg gcatatcatc ggcaattgcc gttgccaggc
    23041 gaatcgggcg acctttcacc cgtccgctca ttaattgcag ggtactttcc agcagcgggc
    23101 gcggttcaaa gggctcatcg ctgaccgaaa cattcttgcc acctgcttcg atagcggaat
    23161 aatcgagaat atcgttgagg atggtcagca acgattcacc agaatcagta attgcccgca
    23221 aatcatcacg ctgggcgtta agtgcggggt tatctgccag cagttgagca gtgccgagaa
    23281 taccgtacag cggtgtgcgg atctcatggc tcatcgccgc cagaaacgcc gattttgcct
    23341 ggctggcttt ttctgcttcc gcccgtgcct gtcggtgttc tatcaccagt tcctgcaatt
    23401 cagctgtacg cgctttgacc tgcgccgcca gctgttcgcg gtggcgattc agtgcatgaa
    23461 cattgctgcg aaacgcatcc atcagccgcc cgatggtatc cagctcccgt acgccagcgg
    23521 tttccgggaa aggggagtca atatcaccgt ccagcagccg ttgcagcgcc tgcgtttgtt
    23581 cggcaagtgg acgcgtgact gagcgataaa ccacgcgcca gaggatcaga atcagtgcgc
    23641 aaagagaaac tattcccagc aataacaggc tgtattgccc gcgtgcactc gctttttcca
    23701 gatgcgccag tccgtgctga ttacgcagct caatggtatc gaccagctga ctgacttcgc
    23761 tactaaactg cgcgaactgg gcgatgttat tttgggcgag agtttgcagg tgagtgctga
    23821 tttcactgtc ctgctgatac agcgccagta gatcgctata ttggctaacg gtagttaacg
    23881 ttgttgcgac ctgcgcacga actcccggat cttcaatgcg tatttgccga cgttgcagaa
    23941 ttttcaccgc attattgagc tgcttttcca gcgttggtgc atttttctgg atctgctcca
    24001 gccccagatt catcaccatt tgctgcaccc gcagagcgct aaggcgcagt tcattcatct
    24061 ggttaacata ctcaagatcg atatcaatca gccgatcgag tgcactttca gcagcctgac
    24121 gctgatcttg ttcgatcaaa tcgtaaatcc cggcctgggt cgctccagcg gaagttgtcg
    24181 cattattcgc ctgaccttgc gccaggcgtg cgatctcatc ggcggcagcg actatctgct
    24241 gactgagttg ccgttgttgc tggcgtagtt gcagacgccg ccccaccagt tccccttgct
    24301 gacgtaatga acgggagatc tcctgctcct gttgttcaat agcggtggta tcaaaacctt
    24361 gttcccgtaa cgcttgcagc aacgcattaa tcttcaggct ttgtgcggtg agcattcgcc
    24421 cctgcgcctg ccacatcttt tcgttatcgg cactggtcag gttctgcgcg gcgaaaagtt
    24481 cccaggcgct ggcttcgctc aactggcgcg ccatattcat ggtaggaatc aatgcctgag
    24541 tgttgtcttt ttccacctgg ctgataaagc gcaggttgta ccatcccacc agggtactgg
    24601 tcagggttaa cagcgccatc agggcaaagc ccatccagag tcttcgggtc agggttaaat
    24661 tcacggtcgg tgcacttaag gtgaaaaagg ttgagtcgca aagcggaatg catctagcat
    24721 aaagccttat tattgatgag gctatcatgc gcgtactgct atttttactt ctttcccttt
    24781 tcatgttatc ggcattttcg gctgataacc tgttgcgctg gcatgatgcg cagcattaca
    24841 cggtgcaagc ctctacgccg cttaaagcca aacgcgcatg gaaactgtgc gcgctttatc
    24901 ccagcctgaa agattcatac tggttatcgt tgaactatgg tatgcaggag gcagctcgcc
    24961 gctatggtgt ggatttaaaa gtgctggagg caggcggcta cagccagttg gctacccagc
    25021 aagcacaaat cgaccagtgt aaacagtggg gcgcagaggc cattttgctc ggtagtagca
    25081 ctacgtcatt tcccgacctg caaaagcagg tagcaaatct gccggtgatc gaactggtaa
    25141 atgctcttga tgatcccctg gtgaaaagcc gcgttggtgt accctggttt cagatgggct
    25201 atcaacctgg gcgatatttg gtgcaatggg cgcacggtaa accactgaac gtgctgttga
    25261 tgcccggacc cgataatgcc gggggcagta aagagatggt cgagggtttt cgcgcagcca
    25321 ttgccggaag cccggtgcgt attgttgata ttgcgcttgg tgataacgat attgaaatcc
    25381 agcgtaacct gttgcaggag atgctggaac gccacccaga aatcgacgtc gttgccggaa
    25441 cggccattgc ggcagaggcg gcaatgggtg aagggcgtaa cctgaaaacg ccgcttaccg
    25501 tggtgtcgtt ttatctttca catcaggtgt atcgcgggct gaagcgggga agagtgatta
    25561 tggctgccag cgatcaaatg gtctggcagg gggaactggc ggttgagcag gccatcaggc
    25621 aattacaggg gcaatcggtg tctgataatg tcagcccacc gattttagtt ctgacgccga
    25681 aaaatgccga ccgtgaacat attcgccgct cgctgtcacc agggggattt cgtccggtct
    25741 atttttatca gcacacatca gcggctaaga aataaccttc accatgttgc gtcaccagta
    25801 aatccgcgct gagtttatga cgtaaacgac gaattaacac atcgacggtg cgcaggtcag
    25861 ggttttccac ccgacgcgca gaaagcatac gtagcagacg ttcacggctg agaatttcgc
    25921 ccggattcgt cacaaatgcc accaacattt catactctgc gcgggtcagt ttaatcggct
    25981 cgccatcccg ctccagcgta tggcgcgaca cattcaggca ataaccggca aagcgatagc
    26041 agttgtcctg agtgtgcggt tgagcttgtc gcgcgaggtc gattcgccag agcagatttt
    26101 tcacccgtac taccagttcg cgcagttcca gcggtttggt gacgtaatcg tctgcgccca
    26161 tttccagccc aacaatacgg tcaatccgat cgctgcgtcc ggtaaccaga ataatcccca
    26221 ccgttgagcg ttctcgcagg gcgcgggtta acatcaggcc attttcatcg ggtaagttga
    26281 tatccagcag aattaaatct accggctgat tctgcataat ttcccgcagc ccggcaccgc
    26341 tcgctgtaac ggaaacggta tacccctcct gagtgaagta agattgtaat cgcgcctggg
    26401 taaccggctc atcttcaaca ataacaatgt gatgtggcat cagagggttt tactcattct
    26461 gttcatatct gttcatattc tgccgtaagc cgttcatcct gaccagtgcc gctgttcata
    26521 tttgctcatt aagatcgctt cactaaacca taattttaca ggggctatta tgcggaaact
    26581 ctggaacgcg ctacgccgac ccagtgctcg ttggtcggta ctggcgctgg tcgcaattgg
    26641 gattgtgatt ggcattgcgc tgattgtatt gccacacgtt gggatcaaag tcaccagcac
    26701 aaccgaattt tgtgtcagtt gccacagtat gcaaccggtg tatgaagaat ataaacagtc
    26761 ggtgcatttc cagaacgcct ccggcgtgcg agctgaatgc catgactgtc atatcccgcc
    26821 ggatattcca ggcatggtga agcgcaaact ggaagcgagc aatgatatct accagacctt
    26881 tattgctcac tccattgata cacctgaaaa attcgaagcc aaacgcgcgg aacttgccga
    26941 gcgtgaatgg gcgcgaatga aagaaaacaa ctcggcaacc tgccgctcct gccataacta
    27001 cgatgcgatg gatcatgcga agcagcatcc tgaagcggca cgtcagatga aggtggcagc
    27061 gaaagataat caatcctgca tcgactgtca taaaggtatt gcccaccagt taccggatat
    27121 gagtagcggc ttccgtaagc agttcgatga gctgcgcgcc agtgctaatg acagtggtga
    27181 cacgctgtac tctattgata ttaagccaat ttatgcggcg aaaggcgata aagaagcctc
    27241 tggttctctg ctgcctgctt cggaagtgaa agtccttaaa cgtgacggcg actggctaca
    27301 aattgaaatt accggctgga cggaaagcgc cggacgtcag cgtgtactca cccaattccc
    27361 aggtaaacgc atctttgttg cctcgattcg tggtgatgtg cagcagcagg taaaaacgct
    27421 ggagaaaacc accgttgccg acaccaatac cgagtggagc aagttgcagg ccactgcgtg
    27481 gatgaagaaa ggcgacatgg tgaacgatat caaaccgatc tgggcttatg cggattcgtt
    27541 gtacaacggc acctgtaacc agtgccacgg tgcaccggaa atcgcccact ttgacgctaa
    27601 cggttggatc ggcacgctca acggcatgat tggctttacc agtctcgata aacgtgaaga
    27661 acgcaccttg ttgaaatatc tgcaaatgaa tgcgtctgac accgcaggta aggctcacgg
    27721 cgataagaag gaagaaaaat aatgaacaat aacgatctct ttcaggcatc acgtcggcgt
    27781 tttctggcac aactcggcgg cttaaccgtc gccgggatgc tggggccgtc attgttaacg
    27841 ccgcgccgtg cgactgcggc gcaagcggcg actgacgctg tcatctcgaa agagggcatt
    27901 cttaccgggt cgcactgggg ggctatccgc gcgacggtga aggatggtcg ctttgtggcg
    27961 gcaaaaccgt tcgaactgga taaatatccg tcgaaaatga ttgccggatt gccggatcac
    28021 gtacacaacg cggcgcgtat tcgttatccg atggtacgcg tggactggct gcgtaagcgc
    28081 catctcagcg atacctccca gcgcggtgat aaccgttttg tgcgcgtgag ctgggatgaa
    28141 gccctcgaca tgttctatga agaactggaa cgcgtgcaga aaactcacgg gccgagtgcc
    28201 ttgctgaccg ccagtggttg gcaatcgacg gggatgttcc ataacgcttc ggggatgctg
    28261 gcgaaagcta ttgccttgca tggtaatagc gttggtacgg gcggagatta ctctaccggt
    28321 gctgcgcagg tgatcctgcc gcgcgtggtt ggctcaatgg aagtgtatga acagcaaacc
    28381 tcctggccgc tggtattgca gaacagcaaa accattgtgc tgtggggctc tgatttgctg
    28441 aaaaaccagc aagcgaactg gtggtgcccg gatcacgatg tttatgaata ttacgcgcag
    28501 ctaaaagcga aagtcgccgc cggtgaaatt gaggtcatca gcatcgatcc ggttgtcaca
    28561 tccacccatg agtatctggg gcgcgagcat gtgaagcaca ttgcggttaa cccgcaaact
    28621 gatgtgccgc tgcaactggc gctggcgtat acgctgtaca gtgaaaacct gtacgacaaa
    28681 aacttcctcg ctaactactg tgtgggtttt gagcagttcc tgccgtacct gctgggtgag
    28741 aaagacggtc agccgaaaga tgccgcatgg gctgaaaaac tgaccggcat tgatgccgaa
    28801 accattcgtg ggctggcgcg gcagatggcg gcgaacagaa cgcaaattat tgctggctgg
    28861 tgcgtgcagc gtatgcagca cggtgaacag tgggcgtgga tgattgtggt tctggcggcg
    28921 atgctggggc aaattggcct gccaggtggt ggctttggtt ttggctggca ctataacggc
    28981 gcaggcacgc cggggcgtaa aggcgttatt ctgagtggtt tctccggctc tacgtcgatt
    29041 ccgcctgttc acgacaacag tgattacaaa ggctacagca gcaccattcc gattgcccgt
    29101 tttatcgatg cgatcctcga accggggaaa gtgatcaact ggaacggtaa atcggtaaaa
    29161 ctgccgccgc tgaaaatgtg tatttttgcc ggaactaacc cattccatcg ccatcagcag
    29221 atcaaccgca ttattgaagg ctggcgcaag ctggaaacgg ttatcgccat agataaccag
    29281 tggacctcaa cctgccgctt tgccgatatc gtgctgcctg cgaccacgca gtttgagcgt
    29341 aacgatctcg accagtacgg caaccactcc aaccgtggca ttatcgccat gaaacaggtg
    29401 gtgccgccgc agttcgaggc gcgcaacgac tttgatattt tccgcgagct gtgccgtcgc
    29461 tttaatcgcg aagaagcctt taccgaaggg ctggacgaaa tgggctggct gaaacgcatc
    29521 tggcaggaag gtgtacaaca aggcaaagga cgcggcgttc atctgccagc gtttgatgac
    29581 ttctggaata acaaagagta cgtcgagttt gaccatccgc agatgtttgt tcgccaccag
    29641 gcattccgcg aagatccgga tctcgaaccg ctgggcacgc cgagtggcct gattgagatc
    29701 tactcgaaaa ccatcgccga tatgaactac gacgattgtc aggggcatcc gatgtggttt
    29761 gagaaaatcg aacgctccca cggtgggccc ggctcgcaga agtatccgtt gcatctgcaa
    29821 tctgtgcatc cggatttccg acttcactcg cagttatgtg agtcggaaac tctgcgtcag
    29881 caatatacgg tagcgggtaa agagccagtg ttcattaacc cgcaggatgc cagcgcgcgc
    29941 ggtattcgta acggtgatgt ggtacgcgtc tttaacgctc gcggtcaggt gttggcaggg
    30001 gcagtagttt ctgaccgcta tgcacccggc gtggcacgaa ttcacgaagg ggcatggtac
    30061 gatccagata aaggcggcga gcctggtgcg ctgtgcaaat acggtaaccc caacgtgctg
    30121 accatcgaca tcggtacttc gcagctggcg caggcgacca gtgcgcacac tacgctggtg
    30181 gaaattgaga agtacaatgg agcagtggag caggtgacgg cgtttaacgg ccctgtggag
    30241 atggtggcgc agtgcgaata tgttcccgcg tcgcaggtga aatcatgacc acgctgacag
    30301 cacaacagat tgcctgtgtt tacgcctggc tggcgcagtt gttctcccgt gaactggacg
    30361 atgaacaact gacgcaaatc gccagtgcgc agatggctga atggttttcg ttgctgaaaa
    30421 gcgaaccgcc gctggctgcg gcggtgaatg agctggaaaa ctgtattgcc acgctgacag
    30481 tacgtgacga tgcccgtctg gaactggccg ctgacttttg cggcctgttt ctgatgaccg
    30541 acaaacaagc ggcgctgccg tatgcatcgg cctacaaaca ggacgagcaa gagattaaac
    30601 gcttgttagt tgaggcaggg atggaaacca gcggcaattt caacgaaccg gcagatcatc
    30661 tggcgatcta tctcgaattg ctcagccatc tgcatttttc gctgggagag gggaccgttc
    30721 ctgcgcgaag aatcgacagt ttgcggcaaa aaacactgac ggcgctgtgg caatggttac
    30781 cagagtttgt tgggcgttgt cgtcagtatg acagctttgg tttttacgcg gcactaagcc
    30841 agttattgct ggtgttagtg gagagcgacc accaaaacag ataacgtcgt ttgtgcgcct
    30901 gaaaagacgc gtttagcgtc gcatcaggca ttatggggca gttgccggat gcgtcatcac
    30961 atccggcaaa cacacatcac ggatgagtta caaaccggga aagtcgctgg cgcagcaggc
    31021 ggttttcctg cttcaggtgc gcaatatcat ccattaacgt cagcgccacc gcgatcccag
    31081 gccagtccag agccagttca tgacgcaggc gtactgcgcg ttgcaccacg atggcggcat
    31141 ggtcgtcaaa tacccaggtt gtttcctgga tctcacgcgg ttcaaccacc cccaaaccga
    31201 caatttcatt caactcctct tcagagatgc cggtatgcag gcaaaattcg gtaatagtaa
    31261 aagtcaccgt aacattagcc attatgcttt cccccaatct ttacgtggat caaaagacga
    31321 ctgggcgtct gccagttgct gccacagcgc ggcagtgttt tcatccggtt tcggcggcat
    31381 cacgattttc agtaccgcat acagatcgcc ggtctgtttt ttgctcacca gacctttgcc
    31441 tttaacgcgc aatcgttgcc cggcctggct gcctggcggg atagtcagca aaatgctttc
    31501 tttcagtgtt ggaacggtga ctttagcacc cagcgccgct tcccacgggc taaccggcac
    31561 tacaatttcc agatcatggc cgacaatatc gaacagcgga tgtggcgcaa tatgaatcac
    31621 cagccacaaa tcgccatttg gaccgccgtt ttcgcccggc gtcccctggc ctttcagacg
    31681 gatgcgttga ccattgccga cgcccgccgg gatcttgaca ttcagcgttt tcggaatttc
    31741 ctgttcgatc atgccaaagg cgttataaac cggcaggtta tagctgatgg tacgcttatg
    31801 ctcagtaagc gtttcttcga ggaataccgc cacttcgatt tcaatatcgt ggccgcgtgt
    31861 ggcggggcgt tgacggctct ggcgggcatg ctgaccgaaa attgacgaga agatatcgtc
    31921 aaaatcttcg gcgttaaaac tctgaccgtc gctatgatgg aactgacggt taaattgcgg
    31981 atcgttgcga tgttgccaca tctgatcata ctcagcgcga cgttgttcat cacttaacac
    32041 ttcccaggct tcagcgacct ctttgaagcg ggcttcggca tccggttctt tgctgacatc
    32101 aggatggtat ttgcgggcca gtcgacgata ggcggtcttg attgtcttga gatcgtccgt
    32161 cggtttcacg cccatgatgg cgtaataatc ctttaattcc atagcgttat ctcgcgtaaa
    32221 tcaacacaaa ttgaaggaac ccctgtaagg taactcctat aagtgtaggg taatcctcaa
    32281 aatttcatat gccaacacag aatatgttat tgaaatcatc gcggagagga ggtcgcctca
    32341 aggtggggtg ctgaacatat tttaaacagg tgaaaaaggg tgagcgattt ttgatagtgg
    32401 aaccaggcac tttaagttta actggggcgt cattatttat taaattttat aggcgctata
    32461 tatgagtagt aatatacatg gaattagttg cactgcaaat aattatttga aacaagcctg
    32521 gaacaatata aaaaatgagc acgaaaaaaa tcaaaaatat tcaatcacgc tttttgaaaa
    32581 cacactggtg tgttttatgc ggttgtataa agaaatcaga cgtcaaaaag ctgaagacta
    32641 cattccatgc ctggaatgcg attcactaga aaaagaattt gaggaaatgc agaatgataa
    32701 tgatttatca ttatttttga gaacattgcg tactaatgat acagaaactt attcaggggt
    32761 ttcagaaggt attacatata ctatacaata tgttcgagat attgatattg ttagagtgtc
    32821 cttgccgggc agaggttcag agtctatcac agattttaaa ggttattatt ggtatggctt
    32881 tatggaatat attgaaaaca ttaatgcgtg tgatga
//
LOCUS       DAZGJW010000042        32750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZGJW010000042 DAZGJW010000000
VERSION     DAZGJW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.042"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(186..2207)
                     /gene="fyuA"
                     /locus_tag="ACHKIK_001994"
     CDS             complement(186..2207)
                     /gene="fyuA"
                     /locus_tag="ACHKIK_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HFO9083173.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIEDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPDGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
     gene            complement(2338..3903)
                     /gene="ybtE"
                     /locus_tag="ACHKIK_001995"
     CDS             complement(2338..3903)
                     /gene="ybtE"
                     /locus_tag="ACHKIK_001995"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HFO9083174.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETMSDDFTPLFSLHGER
                     QPDVSTTALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLPVAHN
                     FPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQAREWE
                     DSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHATILH
                     SQGRPLSPLDEICIVDQDENDVAPGETGQLLTRGPYTISGYYHAPAHNAQAFTAQGFY
                     RTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAAPDTL
                     LGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKIDKKRL
                     TALAVDRYRHSAQ"
     gene            complement(3907..4710)
                     /gene="ybtT"
                     /locus_tag="ACHKIK_001996"
     CDS             complement(3907..4710)
                     /gene="ybtT"
                     /locus_tag="ACHKIK_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HFO9083175.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            complement(4707..5807)
                     /gene="ybtU"
                     /locus_tag="ACHKIK_001997"
     CDS             complement(4707..5807)
                     /gene="ybtU"
                     /locus_tag="ACHKIK_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HFO9083176.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDMVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLYVADHQQNPHSLWRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSNLAGEHPPEACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            complement(5804..15286)
                     /gene="irp1"
                     /locus_tag="ACHKIK_001998"
                     /pseudo
     CDS             complement(5804..15286)
                     /gene="irp1"
                     /locus_tag="ACHKIK_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
     gene            complement(15374..21481)
                     /gene="irp2"
                     /locus_tag="ACHKIK_001999"
     CDS             complement(15374..21481)
                     /gene="irp2"
                     /locus_tag="ACHKIK_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HFO9083177.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDVARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLQPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     ALAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAYWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLVGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPDFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTQRPRPVRRRQRRI"
     gene            complement(21672..22631)
                     /gene="ybtA"
                     /locus_tag="ACHKIK_002000"
     CDS             complement(21672..22631)
                     /gene="ybtA"
                     /locus_tag="ACHKIK_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HFO9083178.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHDGFNHEQKPSTLWLQGQSLVMLSLVLDEQREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            22888..24600
                     /gene="ybtP"
                     /locus_tag="ACHKIK_002001"
     CDS             22888..24600
                     /gene="ybtP"
                     /locus_tag="ACHKIK_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HFO9083179.1"
                     /translation="MALAGLAALTSLGALLFLVWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLQTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSEQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFVDTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            24587..26389
                     /gene="ybtQ"
                     /locus_tag="ACHKIK_002002"
     CDS             24587..26389
                     /gene="ybtQ"
                     /locus_tag="ACHKIK_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HFO9083180.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIACPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVYNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWVHE"
     gene            26382..27662
                     /gene="ybtX"
                     /locus_tag="ACHKIK_002003"
     CDS             26382..27662
                     /gene="ybtX"
                     /locus_tag="ACHKIK_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HFO9083181.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFRREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFMMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     LHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            27690..28994
                     /gene="ybtS"
                     /locus_tag="ACHKIK_002004"
     CDS             27690..28994
                     /gene="ybtS"
                     /locus_tag="ACHKIK_002004"
                     /EC_number="4.2.99.21"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     Irp9/YbtS"
                     /protein_id="HFO9083182.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVMGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLSENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(29188..30103)
                     /locus_tag="ACHKIK_002005"
                     /pseudo
     CDS             complement(29188..30103)
                     /locus_tag="ACHKIK_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(30265..30340)
                     /locus_tag="ACHKIK_002006"
     tRNA            complement(30265..30340)
                     /locus_tag="ACHKIK_002006"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30305..30307),aa:Asn,seq:gtt)
     gene            complement(30441..31238)
                     /gene="mtfA"
                     /locus_tag="ACHKIK_002007"
     CDS             complement(30441..31238)
                     /gene="mtfA"
                     /locus_tag="ACHKIK_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO9083183.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            31332..31421
                     /locus_tag="ACHKIK_002008"
     tRNA            31332..31421
                     /locus_tag="ACHKIK_002008"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31366..31368,aa:Ser,seq:cga)
     gene            complement(32090..32740)
                     /gene="zinT"
                     /locus_tag="ACHKIK_002009"
     CDS             complement(32090..32740)
                     /gene="zinT"
                     /locus_tag="ACHKIK_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO9083184.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPIFQKKADADKTKTFAEIKDYYRKGYV
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         6883 a         8977 c         9731 g         7159 t
ORIGIN      
        1 tttgatatga ttatttcttt agtataagga gagaaaggaa cgagaaagtt aaatctattt
       61 atcctaatcg caatttcatt ttttaaataa tcactatgga ttaatatttt ttgcgactct
      121 gctgagttac tggttttaac tagtggtgta aaagggatac cttttcggta tcccttttac
      181 aataatcaga agaaatcaat tcgcgtattg ataccgacgg tgcgacccat attgacctgc
      241 gcgacggcgc tgctgccgtt catgtagcca taggtacggt aacgacggtc gaacaggtta
      301 tcgacataga cggaaatgtt catccgttca gtcgcctgcc agcccaggct gctgtccagg
      361 gtggcatagg tgccttgccg caactggttg tcgccatcga aataatgcgg cccgaccaga
      421 ttaaccgcca gtcggggcat cagtgcgcca tagcgcgtat caatcacgcc gttcacgctg
      481 cttcccgcgc cataacgtgg tacgaacggc acccggttac cgtgatacaa ctcactgtca
      541 ttggtgaatt cggaacggat cacgttgcca ttgatatccc atgaccagcc tggcgcaaac
      601 cgccacttcg cttcaagctc aacgccggtg gcgtcggctt tacccgcatt gcttaatgtc
      661 tgcatcccgt ccgggccaga gtaaagctgc atgtctttgg tgtgggtata aaacgtcgcg
      721 gcttgcagcg tgacgtcagc ggtttcgtag cgggtgccaa gttcatagtt gatggatttc
      781 tcggcgacga acggtttggc atcaagaccc gcagtaggca cgatgttgta cccggaaggt
      841 ttatatccct gggctacacg ggtatacact ctccagtcat cggtcagcat atagcctgcg
      901 gatagctgcc cgagcacctg atcgtcattg ctcttaccct ggtcgccaaa cgggttgccg
      961 agcatgctgc cgtgatattg tgtactggat ttatcatgcg agaagcgcac gccgccgccg
     1021 atatcaaaac gatcggttaa atgccaggtc aggtcactgt atgcggccag cgtttcagcg
     1081 gtggtatagc cggtactgct taaataaggc attgtcggca tgtcgtaggc tgaatttaac
     1141 ttctcgcggg tgttctgccg gtacagccca aacaccatat caacggtacg cgcatcgccc
     1201 agggttgcgg cgcgcagctc ctgcacatcc tgattccagc gctgaggcat attgacgatt
     1261 aacgaaccgg aagggaaggt gcgcgaataa tgctgctgct gccaggcgct gatcaggttg
     1321 aaaacccagt catcggtggt gtatttccca ctcagggttt ggctgtcagt gcagcgccgc
     1381 atgtacgggt ctggtgaacc atcgctgatc gacagcttac ggcccttaat atcattccat
     1441 cccacatagg catcctgggt ggcgcgagta cattcgcgtg aggcggcaaa gcccatttcc
     1501 cagggctgat cgtccggcgc cagacgcagt ttcacattcc ctatgctggc gcgggtgccg
     1561 cctaagtcat cgcttcccgt cgcggggtta atcatgtcgc cgtcatcaac ctggcgtaac
     1621 agggtgacgc tgccgtacag caggccatcc tcaatggggc cgctcaggtt gaacttactt
     1681 cgataactgt cgcggctact gacgccgcct tcaatatagc cgcgcggcgt gctgtccggc
     1741 tgctgggtga cgatgttgat gatcccgccc tgagcgcttt tgccatataa cgttccctgt
     1801 gggcctcgca gcaactccac gctttgcaca tcggtaagcg cctggatggt gttggtggaa
     1861 agctgaggga cgccatcgac atacagggtg acggcggggt tatagaagtc ctgcgctgaa
     1921 gagacgccgc gtagcgagat cgtcgaaaaa agcatgttgc cgctattttc aatattgagc
     1981 ccgggcaaga ctctggggag tttgtcgctg gcggtgacgc cggcgtcgct taattccggc
     2041 gcgctgacaa cagtagacga gacgttgttg gctgatgccg agcgggaaga ttgtttactg
     2101 gcggtaacca ccagcgtgct ttcgtcttgc tgtgaagtct gggcattagc aatggcgggg
     2161 agcaataatc cccccaaggc cagaggataa agccgtgtca ttttcattgt tgtgagtccc
     2221 tgtaagacgg cgaaacgcca aaaataccga caatggtgaa ttgaaaatga taactatttc
     2281 taatagtggt gctacccgaa acgggtttat ttcaacctgt ttcgggtcgg tttgcgctta
     2341 ttgggcagaa tggcgataac ggtcgacggc gagagccgtc aggcgttttt tgtctatctt
     2401 gccgacggcg gtgagcggcc agtggtccag aaactcgatt tggtcaggaa ttttccacgc
     2461 gctgagcccc atacgggtca gttgttgacg caactgctga tagtcagttg gcacctgctg
     2521 cgcgatgata aacgcgcaaa tccgctcgcc aagcagcgtg tccggcgcgg cgaccaccgc
     2581 gcaatcttgc acttccgcta aacgcagcag tgccgattcc acttcagccg cggctatttt
     2641 ttctccggcg cggttgatct gctcttttat gcgtccctca acgtgcaggt tccccacctc
     2701 atccagcctg acattgtcgc ctgtgcggta aaacccttgc gcggtaaagg cctgcgcgtt
     2761 gtgggcaggg gcgtggtaat agcccgaaat ggtataaggg ccgcgcgtta acaattgccc
     2821 ggtttcgccc ggcgcgacgt cgttctcgtc ttgatcaacg atgcagattt catccagagg
     2881 ggacaacggg cgcccctggc tgtggagaat ggtggcatgc ggatcgtcca gccgggtaaa
     2941 acagagcagg ccttccgcca taccgaaaac ctgttgcagg gtacagtcaa aggtggcgat
     3001 aacctgctca gcaagcgtcg ggtcgagccg ggcgccgcct gcctgaatga cgcgcagcga
     3061 cgaaaggtcg ctgtcttccc actccctggc ctgcacccat aattgcgcca gcgccggaac
     3121 cagggcgacg tgagtcactc tttcctgcgc gattaaaggc atcacctcat cacagctggc
     3181 gctgtcggtc agcaccactt ttccgccgca ggcaagcgtt cccagaatac cggggcaggc
     3241 cagcggaaag ttatgcgcca ccgggaggac ggcgagatac acgctctgtt ggctgatgcc
     3301 gcacagttca gcagaagcgc tgaagttata gctatagtcg gcatgtcggc gcgggatgag
     3361 tttgggcgtg ccggttgtgc cgcctgagag caacaacaac gcggtggtgg aaacatcagg
     3421 ctgtcgctca ccgtgaaggg agaagagcgg cgtaaaatcg tcgctcatgg tctctccagc
     3481 gaccagaaca tgacgcaagc aggcgtgttt gtgtgccatc tgtcgggcca gctctgcgtg
     3541 gttttccccg tgaataacgt aagcgacggg ttgcgccagc tcaatcagcg cgtcgatatc
     3601 cagcgcccgt tgcgagggca tcgccagcac ggggataacg cccagtctta acaaggcgaa
     3661 cagcagggta acaaacgcgt tgtcgttggg aagctgcaca attacatgct cccccgaacg
     3721 caaacccagt gatgacagac ctgcggcaag ttcgtcaact tgtgcatcaa gcgcgctgta
     3781 ggttaacgac ccctttgcat caacgacggc aattcgcgag gcataacggg ccgcccagtg
     3841 gcgcaactgt tcggcaatgg gtaagggata ctgttcaatc agagattcaa aggaagaatt
     3901 catgcgtcac ctttctgaag tactgggctg tttttgccac gcggtcattg cagaaaatgc
     3961 gtggggaaaa tggcggacaa tctgcgtaaa aaaggaccgg gcttgttgaa tgggatagaa
     4021 gtgatcgccg tcaatcacca ccgggcctgt aacgtggctc agccactgac gccaggcatc
     4081 gacctgctgc caggaggctt cgcgatcgtg gctgccgcac aataacagcg caggcgtgcg
     4141 cagcggcgga cagacgtcgg gcgagtcgta gtgatagctc tcggtggcgt aaaaatcagc
     4201 gcgcagaaga ggaagaaaca gcgacattaa ttcctggttt tcccgcagtt caggagaaca
     4261 tccgccaatg tctatcagct cagcgataaa gtcggcatca tcgcgatggc tgagctggcg
     4321 ttcagaatgc agatgcgggg catggcaccc ggaaataatc agtccttgtg gcgcaagccc
     4381 ccgttgctcc agaagtcggc aggtttcaaa cgccacctgc gcccccatgc tgtgcccggc
     4441 gagtaaaagc ggcgtgtcag gcgatacgga tgcttccagc tcgttcgcca gcagtgccgc
     4501 cagttgtgta atgcttctga gcggttccag atggcgaagg cgatcgcgcc ccggccaggt
     4561 caccagagaa agcgcgcaat cagccagttg ctcagcttgc cagtggcgaa acgcgctact
     4621 gctgccgcca gcgaaagggc acatgaccag atgcgcagta ttgccgttcc gggcgggcca
     4681 cagcgggatg cacattgcag attgcgtcac agcgcctcct tatcatcatc gttgtagaaa
     4741 ccggctaacc ggtcgtggtg cggcggcgtc agacgtctga tctccgcatt gccggtctta
     4801 cgtaaaatct gctgccataa acgtgataac gcgatctgat gtacgctttg gcaggcctct
     4861 ggtggatgtt cgcccgccag attgctgcgc aactggtgta gcaaccagct gacgccctcc
     4921 ggcccgacgg tttcgcagca gtcgcgccag ctcaggggcg ccgcgctgcg cgtcagaccc
     4981 ggcagatcgc gcaggatctc cggtctgcgc cagagactgt gggggttctg ctgatggtct
     5041 gcgacataca gactggatga ccagatgacg gggccgtagc tcgcctccag cgaaagatga
     5101 ccttccggcc agcccagcag caggcggtgc atgataaggc tatgcatatc tggatcgtca
     5161 ggatcgagat agcgctgaag cagcaggcag gcttctccct ccggccagaa gagtctcaga
     5221 cagtgaaaat cgctaaagct gccaaccatg tcgcactcca cggcggcggc atcaacgccc
     5281 agagccagca acaacaaatc cagagtggag tagagcaact gtcggctggt ggtggcgtgg
     5341 actaccggcg gcgtcttagc caggcagcga cgcagctgct gcgcatcgcg aagccatgtg
     5401 cgtcccgcgc gcgtatgcgg ataaaacgtg tttacccaat agcagcagcc ctgttcctgc
     5461 gccagcgttt gtaaggaact gatgtcatcc gggtggaggg gatgctcctg aatgacgtgt
     5521 acgccgcgcg tcagaaagtg tctggcaagc tgcgtaccgg ttcctccggc gacggtcgaa
     5581 cgcacgacaa tacaggcgat atccggcatc ctggttatct gttccggcga ggtatacagc
     5641 ggaatgccaa acgcatgagc cagctctctt gaacgggcgc ttccctgcgc cagcaggccg
     5701 accagttcca gcccctccgg gggctgcata aaggcattca ggtacatttc gccaaatttg
     5761 gcgcccacaa tcagtacgcg ttgttttggg gaggcggacg gcatcataac gtgttctccg
     5821 gttgcgttga ggttgcgcaa agccagcgcg taatgtgttg cgcacaagcc tgaacgtggg
     5881 gggcttccat catgatttgc cagtggctgg cttcaatcac agcgtcgtcg gcgttgttta
     5941 tccagccctg caactcggtt tctgctggcg tccagcgcgc gggtctcccg gcggcataca
     6001 ccatgagaca ggggaccgga acgctttcgc ccggcgtatg ctcagtcagt aaacgcagca
     6061 gatgcgcgat gttgtcgagc cacgtttctg ccgcttgcag cgtcaggttt tgcgacacca
     6121 taccggccgt tttagcgagg ctgataaagt cggcaagctg gctgtcgggc gtctgctggt
     6181 cgaaatgttc gggcagtgcc agaggcgttt ctccttcggc taacaggcgc agcagggccg
     6241 cccggttttc gcaacagaaa tcctgtcggc acacgggatc gattaacacc atccgaacct
     6301 gcttgccttt ggcgtacagg cgctgtgcgg cgcccgccgc aagaaacgcg ccatacgacc
     6361 aacccgccag cacataaggg ccgtgaggct gctgacgacg gatgcacccg acatactcat
     6421 cgatcatctg gtcgagcgta gcaaagcgct gcggcgattt tgcttgcagg ccgaaaacgc
     6481 gcctgttcaa cgcgctagcg agcggcagcc aggcgctgat atcgccgtct gaagcgtgga
     6541 agacaaacag ggtttcctca ccgtcgccct ggcaaagggg tatcgggttg tcctctcctg
     6601 acgtagccgc ctgtagatgg gcgcagaagt cgctcagcgt cgaatggttg aacagatcct
     6661 gaaggttagc cctggcaatc cctctccggt ttagctgcgc gaccatcctt gtcgccatca
     6721 ggctgtcgcc gcccagttcg aaaaagtcgt gatgccttgc cacggggcga gacagcagcg
     6781 attgccagag ttcagccacc tgtttttcca gcgccgaatt ccccccggca actggcgtgg
     6841 gtttggctgg ctcagtcggc gcatcagacg gggcatgcag cggcgtaaat aacgcttctc
     6901 tttgccggat ctgtaaaatg ggcagaccgg tgccaaagcg ctgctgtaaa tagcggctca
     6961 cggctttttt atccgggcga tttacgccag gcgaacgcgc taccagcaga tgctggcgca
     7021 gcggcgacga ttcctgcgcg ggccacgcca gctcgtttgc aaacccggcc tgaacgagaa
     7081 cctcctgcca tgcggagcgg gtgagcatcg gtttctcatc ccggcggcgg aaatcgcggt
     7141 atccgcttaa tccctcaata aagcccacgc tcgccagctg gaatacgctg tttcgctccg
     7201 tcgcttcaac gatcagcaaa cgtccgccgg ctttcagcaa cagttttaat ctgcgcaacg
     7261 tctggacgac atggctggcg tcgtggagca cattgacggc aacgatcagg tcgtaacccg
     7321 cctccgggtg ggcggtgaaa tccagcggct ggttgatgtc gaacaaggca taagacacct
     7381 gcgattcatc atggaaacgg gctctggcgt cattgaggaa ctgcgcggag acgtccgtga
     7441 agtggtacga ctgccgcgtg gccttcagca ccggcgcggt agtggctgcg gttccggcgc
     7501 caacctccag aatccgttct gcgctgcaca aggcggcaat ctgcgcggta tagcgattca
     7561 gacaggcgct ggcggggttg tcgcgataca gcgcgtcggt aacgcgatgc tgctcgttga
     7621 acagcaattc cagcggagaa cactgcccgg agaagagggc gtcgtgccgg gcgatgcagg
     7681 tttccagata ctgcgccagc gcctggctcc attggctttg cgggcacgct tcctgaggct
     7741 caggaatctc gctgagcgga atgcggcagc gccagctttc accttcgcgg attaaccact
     7801 ctctttccgt cagacactgt agccactggc gcagcagacg ctggtgagac gcttgcgcgg
     7861 acagcgcctg tactattgcg ctaaagcgat gggcgatctc cggcgtggta aacaggccgt
     7921 gacgatttag cgtagccgcg atcccctgta gcgcacgcgc ctccagccag cgccaggtcg
     7981 cctccagctc atcaagctgc gcctgctcag gcaacggcag cgccagttgg gtaagttcgt
     8041 gctcatcagg catcgccgct gatggcgaag gatcgtcggc catcacgata tccagcgtca
     8101 acgcgccctg ttcggttatt tccgctctgg cctgccggat gccggggtat tcgagcgtcg
     8161 cctgctcaat atcccgcagg tcgtagtccg cctgcgcgtt cagcggccag cgtcggcggg
     8221 gaatgtgccc ggcgatgccg ctggcgagag tcttcaggct ttcaggggcg gcgatgacgg
     8281 cttgcaggat ggctcaatag tcattaaaca tcgcctcggc agcgccgggt tccagcacgt
     8341 tgtccatgca gtaccagcta aacatcaact cgccgtcgct ctccatgacc tgatgatcca
     8401 gccagacctg cggcgtttgc gtgaatacat agcagggttc gccgaacaga tggctcatcg
     8461 cctgatcgat agtcatgcct tccagcgtca tccccagcat actggtaaac actaccggca
     8521 tcagcggttg acgttgtgat ccgcgcaggc ggcccagctc acggatcacc tcaacaccgt
     8581 tcatttcact gtgcgccata ttttgccaga ggcgctgttg ggtctgttgc atctgctctt
     8641 gcaacgtcac tggcgctgag aagttaaaat caaccagcgt gacggaggta aaatcaccaa
     8701 tcaactggtt gatttgcgga tggatcggct ggcgattgaa gaacgtcagg ttcagcgtaa
     8761 atgttgtggt acggctccag cgctcaaggg tggcggcaaa cagcgtgagc agcgcggcag
     8821 acggtgtgac gccttgctgc tgccagcgct gtttcacggc ctgccattct gtcttgccga
     8881 tcgtcgattt gaaggtggtg aagtgtggcg tttccggggg cgtctcaacc atcggcagct
     8941 ctggcgctaa gggcagttgc ggcagttttt cctgccagta atcccaggca tcgtgccatg
     9001 ccgatgtctt tcggcgcgcc tgttcagcca tcacataatc acggaaggta atagcgagcg
     9061 gcgccagcgt ttcaccgcgc cagacctgcg ccaggtcgtc catcatgact ttaaaactct
     9121 gcacatcaaa ctgcaaaagg tcgaggttca tatgcagacg gtaatggcaa tcgtcgattt
     9181 cgctgaccac cagctcaaaa agaggccact ggtcggcagg caaaacgcga tagctcagtt
     9241 catgccgccg tttttccagc gcgatgcgct gttcttccgg ggaaagcgcg cgcagatcgt
     9301 cacgcgggat gtgatactcc ggcgttgtcg ccaggattcg ctgctgcccg tcggcatcaa
     9361 ccaccatacg caacatatcg tggcgtgcga tgagctggtt ccatgctttc tccagtatgg
     9421 ctagatcgaa ctcatcgtgg cgtttatccc actcaaacag gacgtgacag gcgacgccgc
     9481 catagccaat gaggtgggtt cgccccagcc agtaggcgtg ctgaatgggc gtcaaaggga
     9541 agggcgcata acgctcgtcg gcgtcgtgcc gcaacacttc cggctgcgaa acggcaggcg
     9601 tcgcctctgg cttagaacag ataagctgcg tgagtccatg aggagacaga tcctgccacg
     9661 cccgttccgc attgatgcgt acgcccaggt agtgctgaat atcgctactg agttcaagga
     9721 agagcagcga gtccatgccg agttgcaaca gatcctggtt tggatgcagt gacgccggat
     9781 cgctcagcct tagctgaacc gcaatccgct ttttcagcca tgccatcacc gctgtttcat
     9841 cgctcaggct gccgttaaag gcgttgtcat cagcaggagg aatgaccggc gttgcggctt
     9901 tttctgtggc gctgatgtta aacagagcct gttgtaacgg gggcattttg tcggtaaaaa
     9961 cgcgcatcgc cagtcgccac ggggcgccgc gcatcaccgc ctgttccagg tgccagcagc
    10021 cttcggcatc gcttaacgcg cccatacctc ggctggcgag cgtcgccagc atttccggcg
    10081 tggccgcccg accgctttct ccccatgcgc cccaggcgac agagagcgtt ttcggcgcat
    10141 caagggtgga aaactgctgg gccagcccgt ccaggtagcc gcaggacagc gcatgggcgc
    10201 tctgacccgg cgcgccgagg gtggcggcag cggaagagta gagaataaga tagcgtccgt
    10261 cgtggttgcg cagggtttgc aacagctggc ttgccgcctg cgcttttacc gcgaaaacgg
    10321 cagccagctg gtggtcatca agctcctgca agggcgcgtc agccaatacg ccagcggcat
    10381 gaatcgctcc ggcaatgccg ccgttggccg ccagatcgtc aagaaccgtg gccagttgcc
    10441 cggcatcgcc cacatcacaa cggcagacgc gcgtctgccc gccctccacg tcgcgtagcc
    10501 atgactcatc cacgtgcgat gccagcaggg cgatgcgtcg cgcccctttt tctctgagcc
    10561 agttcacggc aaggcggcct aatccgccaa acgctccggt caccagatgc cagcggctat
    10621 cgccggtaaa cacgtttgcc ggtaattcag ctgcgcatcc cgtattgggc gccagtgaag
    10681 ggagccaaag ggtgtcaccc cgtgcggcga gccagcgctg tgatagtgag actgcgctca
    10741 acccttgatg cagcgtttcc cacggggtgt tttcggcgag atcgatggcg gcaagcaacc
    10801 gttccggctg ttcgttggcc gcgacgcgaa gcaaggccca tagcgcgtga tgggatgcag
    10861 agagtgcttc attttcctcg actcgccacg cgcggcgagt aaccacaatc aatccggctg
    10921 cgctggcggt gagcgctgct atcactttct ccgctaaggc cagcgtatcc tcgctctcct
    10981 caacaatcat cagtcgtgaa gaggcgtgcg gatcgtgaat gatgccgtat tgcgccagct
    11041 cgtcgctgcg cgcaagcgta ccggcgctga agctaaaggt aagcggatgg tcaatgctgg
    11101 cgacgttgag gggcgtccag cgccattgat aatgcgtttg cggcgcagga agaggcgcgg
    11161 cgggcgatag cggcagccac tcgccagcag gatgacgggc ttcaacgcgc atctgaccgc
    11221 gggaaaaggc ttcgttttgc cagcttaagc gaaccttccc cagccactcc ggggcggcga
    11281 cggggggcgg ttccacaggc agagcgtcgc catgacgctg tgaaagcaga cgccaggcct
    11341 cctgccagcg ggcgttaaac tgttcgggct gacctgcgca atcagaccag tcggcgagat
    11401 aatcaccgtt atccgtcagc gcctgcccca acacgggttc tgatggcgcg gtgaatgtta
    11461 cggcgctaac cgcctgaccg ggcagcgtgg cgagaatgat atgttcgctc atcccggcgg
    11521 tcgggctgcc atcctgcggt agccacgcca ctttgctgaa tccggcgtgg cggcactgtt
    11581 gttgccactg agcggtggtg aggaataact caccttcgcg ggcgtcgaga tcctgtagcg
    11641 gaagaaccag cgggccgaaa acgaagtcaa acagacgcat tggctgggtg attttgcgca
    11701 tcagcaggcg cccgcccggc ttgagcaggg ggcgcagatt atcgagcgtg cggccaatat
    11761 ggcgggtggc gtgaatcacg ttcgctgcca cgataagatc gtaagactgt gcctggaaac
    11821 cctgagactg cgcctctttt tcgagatcca gctcgctata cttcacaaaa tcatagtcgg
    11881 cgaatttctg ctgggcgcga cgggtgaaca gcgccgagat atcggtgaaa tggtactcca
    11941 gtgccggaac gccgttgagt tccggcagca gccacgcggt ggtgccgccg gttccgccgc
    12001 caacttcaag aatacgcaac gactggcggg gctgacgcgt ctggacaatg ccgcgtaata
    12061 ccccggcggc gatttggttg aaatagcggc caaagctgaa ttcctgatac agcacttcca
    12121 cgccgtcgga ggcgctttgc gggaagataa tcgccaccgg ttcttccgcg ccgctcatca
    12181 tttcatataa ccgatcgccg gcacgggcga tggtgtcggg aatagcctga aaaccttcac
    12241 aataactggc aagttccgtc agcagtgatt cccgctgttg atgttcaatg gggcgggcgc
    12301 ggacgtatcg cccgtcggtg cagcggtaat cgccgtcgac cacgcagtta ttcagcaggc
    12361 gctggagtag ctgctggtag cggggcagca gacgtccacg gcgcatgatg gtcatggcgt
    12421 ccacgccgtt ctcaatggca tcgccggtac agcgttgtac cagttgatcg acgtagatgg
    12481 cgtgcagtcg cgtggcgcac tgtttaagcg cttccaggcg agggagatcg agcgctgtcg
    12541 cggcgcgact cgccacctcc agcccggcag acagcgctgc gtcggcaggc tcgcaggccg
    12601 gggagacgcg ctctttccag taacgttcag tatcaaacgg ataacatggc gcagcgatac
    12661 gttgtccatc gcccgccagc aggtcggccc acggtagggc gacgccggca gcgtaaagct
    12721 ggagcagggc ctgattgagg acatcgctcg cctctttgtt acgccgggcg ctggctatcc
    12781 agtatgcgtt atcgcggtat tcgcgctgcc cgcaagcaac caactgggca tcgggcccca
    12841 tctccagaaa aacgcgggcg ccgagctgat gcgccacctg aatactctgg ataaaacgca
    12901 ccggctggcg catgtgtcgg cgccagtaat ccgcctggtt gagcgttgac tcatcaatga
    12961 cgtcggcggt gagcgtggaa ataatcggta tttgccccgg ctccgcgtgc agtcccgcgc
    13021 aggcgtcctg gaaccgatcg agtatcggct ccagtaaagc ggagtgcgcc gcaccggtta
    13081 cgctcaggcg acgatagtta atgtcatgct gcgagagcgt ggcgcaaaat accgcgagac
    13141 gggcttccgg cccggaaaat accgtatgtt gcgtaccgtt gttggcggcg agatccagct
    13201 caaactggcg agccagcggc atcagcgtgt cttcgtctgc aaataccgcc accatcgcgc
    13261 cgcttgcgca ctgctgcatt agcgcgccgc gccgacaaac cagtggcatg acctgttcaa
    13321 tcgtatagtg tccgcagaca acggcagcgg caaattcacc gacggaatgc ccaatggcga
    13381 agtctggctt cagtccttca gcacgccagt gcgccgccat cgcgatttca aacgcgacaa
    13441 tcgccggctg cgcccaggcc atattgtcca gctgcgccga atcggggtta aacatcgctt
    13501 cgcgcagtga tggcgtgagc atttcgctac aggcggaaaa acagcgatcc agcgtgtcgg
    13561 caaacgccgt tgagtgctgg tacatcgttt ggcccatagt gtgccagtgc gagccctggc
    13621 cggtaaacag ccacacctgc ttgccgctgg cgccgtggcc gctgtaaacc agcgcccccg
    13681 atttctcacc ggcccaggcg ctgagcgcct cggcggtttc acggtttaat ggcgccgcca
    13741 ggcggaaggg gagatcgaga cggcgcgcgt gcagggctgt gaaggccaga gagctggcat
    13801 ccgcattctc tctcagcgcc ccggcataat ccgtcgccag ccgccgcaac gcgctgtcgc
    13861 tggcggcgct gagcagcagc gcggtacttt ttctgccgct atccgtattg gggaggcgcg
    13921 cgttgagcgc gtcgggcagc gaggcgacga tcatatggca gttggtgccg ccaataccaa
    13981 aggaggagac gcccgcatag cgcatttcgt cctgccatgc ctgtgccgac accggtatgg
    14041 tacaggggct ctcttcaagt ttcagcgccg ggttgggggt gtgaaaattc agtaagggag
    14101 gaatttgccc gcgactgact gccagaacgg ttttcagcag tccggcaatg cccgccgcgg
    14161 tatccagatg gcccatgtta cttttcacgg aaccgagcgc acagcgctga tcctgcgggc
    14221 gaggcgcata gacgttgcgt aacgcttcaa tttcaatcgc gtcgcccagc ggtgtgccgg
    14281 tgccgtgggt ttcaatgtaa cctacctgcc tgtcgtcgat ggccgccagc attaacgcct
    14341 cttcgatgac tgcctgttgc cctgcgacgg aaggggcggt atagccgacc tttctgttgc
    14401 cgtcgttgtt gaccgcgctg gagaggatca ccgagataat cggatcgcct gacagcagcg
    14461 cgtctctcag gcgacgcagc accacgcagc cgagaccgtt accggcccag gtgccctcag
    14521 ccgaggcgtc aaagggacga cagtgaccat caggagagaa aatcattccg ggctggtagc
    14581 ggtagcctgc ctgctggggg aaagagagcg ccacgccgcc ggcaaccgcc atatcggatt
    14641 cgccagcgcg caggctttca caggccagat gcacggcaac cagcgagctg gagcaggcgg
    14701 tctgtaccga taacgccggg ccgtgcaggt tgagtttgta cgcggcgcgg gtggcaatat
    14761 agtctttatc attgcccatc agagattgca gaccttttac ctgcgcgact tctgtcacgt
    14821 tcaatgcttc gcgaccgggg taggtactca tccgggaaga ggcgaaaacg ccggtcttat
    14881 gggggacggc gccgggggca taaccggcat gttccagcgc atgccagacc gcctgcaaaa
    14941 acaggcgctg ctgcgggtcc atcgactccg cttcctgtcg cgaatagcca aacagggtgg
    15001 cgtcgaagca gtcggcgttg tctaacaccg aaccgatatt gacataatga ggatcgtcaa
    15061 tgatggcggc atccagaccg acggccagaa gctcttcgcg cgtaaagcgt cggctgcatt
    15121 cacgaccttc cagcagattt tgccagaacg tttcgccatc cggcgattca ggaaaatggc
    15181 aggcgtagcc gataaccgcg acaggttcgc agtccgggta gtgttgagcg atcgatgcat
    15241 caatggaatc tgctgtcggc gcagaagaga agcgcaagtt atccataatc ggtcccgttt
    15301 cctaagattc acaaagtcaa tggaacgcca tgcgttcgcg atggcgttcc ggggaaaatc
    15361 agtttgcttc gcgctatatc cgccgctgac gacggcgaac agggcgaggt cgttgtgttt
    15421 tctccaccgt ggcgacgccg gccagataat cggccagggc agcaggagaa gggtgggtga
    15481 agaggtcaag caacgtcagg gtggaaaatt cgtgacgttg taacaaaacg tgcagttgca
    15541 ccagattcag cgacgttgcg cccgcctcaa agaagttttc tgcgggcgtg acagactcgc
    15601 caaccacctc gcggaaggcg tcgcaaatca ggctgacggt gctctcgtcc gccgcaatgt
    15661 gtggctgaga gtgctcaggg catacgcgcg gtgagcgctc tgcgcattgc cctgcgtgtt
    15721 taacgggagg caccagcgcg gcgagcggca gttgccagcc gctttcatcc tcggccattc
    15781 ggtttagcaa tgcgcaatac tgttcaaact ggcgctccac ctgcccggca ggaaacagcg
    15841 cggcgacaaa atcccagtta aagcgcagtt cgccttcgga ttcataaatc tggtgatcga
    15901 gccagacctg cggcgtctgg gagatgcccc agaccggttt gagcagatta cgccgggcga
    15961 ggaagttatc ctgctcaaag cccagcgcgc tggtaaagac gacgggcatc ggaacggctg
    16021 gcacgttttg ccgttgcgcc agttgacgca tcacgcggat ggctgacaca tcgcggtggt
    16081 tgaggttctg gctcagccgc tgctgtagtg actgcgcgct gtgcagccag ctttcgccgg
    16141 gatgccagct cagcagcatc agcgaggtga aatcgcccag aatctggttg atttgcgggt
    16201 gcagcggtcg cctgtcgaaa agcgtaaggt taagcgtgaa gtcaggctgt gcactccatg
    16261 cagagagaac cgttgaccac accgacaaca gtacggccga cggggtgaga tgggcgtcag
    16321 ccgcccgttt tttcagccga tgccagcgcg tgctgtccag tgcgccgttc aggcgggcga
    16381 agcgcggtgt ttcaacctcc tgaggcaagc agcgcagcgg caacgcaggc gccggaggaa
    16441 tatcatcaag ctgcgcctgc caccatgcca gagaatctgg attgggcgac tgtagcgagg
    16501 gttgttgcag ataatccctg aaggtgacgg gcagcggcgg aagcagctgt tgcgggtagc
    16561 ggtagccgtg ctccagctcc gccagcagga tctgcatgct cagaccgtca agcaacaggt
    16621 tatccagaca cagccacagg cgggcaggca tcccgtccac gtatccgacc tggagatcga
    16681 ataccggcca tacttcgggg ttgagtacct gatgcgccag tttttcgcgc acccgcaacg
    16741 cctcttcagg cgtatggagg gtgtgtgcgg gtatcaccca ggggggcgtc tgttcgagca
    16801 cctgttgctg tccatcacgc acgatggcgc gcagcatatc gtggcgggcg attaatcggt
    16861 tccagaccgt ctccagccgg gtgaggtcca gatcggcaat ttcaaattca acaaagaaat
    16921 gtgagccgac gccgcccagg gcaaagcccg gctgacgccc caccaggtaa gcctgctgca
    16981 cgtcggtaag cgcaaagggc tggtagcgat cttcagggga gtggacgaat ggttgttcga
    17041 ccgggacgtc ggttttccgc agcgtggcgg caaaatccgc cagccgggga tgattaaaca
    17101 ggtcgcttaa ttgcgcttca taacctgcct gatgaagttg cccggtcaga cgggtcgcca
    17161 gcaggctatc gccgccttgc tggaagaagt cggtttctct ggtgacattg ccagttgata
    17221 agagttgctg ccagagggcg gcaacctgtt tttcaatgtc gccctggggt aaatccgctt
    17281 cggccgggtt ttccgcttca ggggtatgac gacgcttcag cgcctggtag tcaattttcc
    17341 cgttagctgt cagcggtaag gcgtcgagga agacgatccg ttgcggcacc atccaccccg
    17401 gcagacgccc ggcgagggcg gcctgaagtt gacggggatc gcggcgcacc tgcctgtcag
    17461 gacattgtac gaggaagcgt tgtaaccccg cgacctcatc gccatgctga cagttgaagc
    17521 cggccgcgct aaataatgcc gcccagtcgg cgctgttatg cagcaaggcc tccggctgcc
    17581 cattggttaa caggagcgtg ctgagtaggg cggacggcgt taactggcga aactccatca
    17641 cgtagagcag cgcgccggga acggcaagct gttgtaatgt cgcaaggagc ccgggatctt
    17701 ccggcagcag acgatgcagg gcgttattaa gccagataat gtccgccgag tgagcgtgcg
    17761 ccgccagcgt gtctgcattc cagagggaca gacgggcgcc aggccagggg gcgagcctct
    17821 gccgggcgct cagcagcatc tcctggctct gctcaagccc gacatactca atctgtccgg
    17881 cgttgagctg tgctaacagc gattctgcgg cgcggccagt gcgggttccc acctcaagca
    17941 ggcgtaccgg gcgctgtaag cgctgtgcaa gctgttggca aattgtcgcc agttcctgaa
    18001 tatacggggc gctggccgga tggttaaagg ccaggctttc cggcgaccac tgcgcgtcgt
    18061 tcagtaacga cagttcgtta cgggcaccgc gaagaatttg cgataacgtt aaatcatgtc
    18121 cgctgaagtg cgggtgctca tcctcgcgtt cttcaccagc gcagacctga taaccttcag
    18181 cagcgggctt cagtcgccgt tgtgtcacca gaaatgctaa ccagcgttcc accacggcct
    18241 gccagcgcgg ctggatagcg agtgagttca tcagggggag aggatcggcg ccagcggtgt
    18301 gacccggctt cagttttagc aggcgatgct gaaggaaatc ggctacctgt tctgcggaga
    18361 tctctggcga gatggcgcaa cagggcaacg ttcccgcaag cgtgtgccac gcctgcggca
    18421 gtgccgggtt ccgatgatcg gtaacgcaaa aagcctcgcc ctgaggaaca acgtatgccg
    18481 ccagcgtttt ttctttttcg ccgatcgcca gaacggttgc ttgtttcacc cccaccagct
    18541 ggctgagcgc gctttcgatt tcgcccagct cgatgcgata tcctccgact ttgacctgct
    18601 tgtcgcgacg accgaggaac tcgattgtgc catctggcca gtagcagccg agatcgccgg
    18661 tgcgatacca gcgctcgtcc gggagcgtca aaaattgctg ctcgctacgc aggggatcgt
    18721 tgaaatagcc ttccgcgacc ccaatgccgc caatccataa ttcacccggc acccagtcag
    18781 ggcagtcccg gccctgttca tccaccaccc ggtagcgttg gttggttagc ggaaaaccgt
    18841 aagggatgga gcgccagtag gcggggacgt cgtgaatttc gcaggcgtta gaccagatag
    18901 acgcctcggt ggcgccgccc atcgcgataa attgtccttg tggccggaag gcccgataac
    18961 gggcggggag gtcaagcccg atccagtcgc cggaaagcat cactgcgcgc aggttttccg
    19021 gcgtggcgtc ggcgaaacct tcacaccagg tcagcagcat atcgaacagc gccgggacgc
    19081 tgttccagag cgtgacctga tggcgctgga tcagctcaca ccatgcgtga ggatcgcgcc
    19141 gttgattttc catcaccatc accagcgcgc cgcccgcgcg cagtacgcca aaaatgtcgt
    19201 aaaccgataa atcaaaatgt agggcggaga gggccagcac cctgtcatgc gggccaacct
    19261 gatagcgggt attgatatcg caacaggtgt taagcgctcc ccggtgagaa atgactaccc
    19321 ctttcggcgt accggtagag ccggaggtgt agataatgta ggccggttgc gtgggggcgc
    19381 gtactaccgg gttggcgatc ggctccgcct caatggcctg ctgccaggca agggcgggaa
    19441 tatcgtctga cccggcgctg gcgtcgtgct gacaaatgag caccagccgg acgctggcgt
    19501 cagcgtagat tttctcgcgc cgtgcggcag gctgatccag cgaaaccgga acgtaaaccg
    19561 ccccggccag caggacggcc agaaccgcaa caagttgtcc tgcgcctttc gacatcgtga
    19621 tagccacatt atcgccgggc tgaaccccgc actcgattaa cctgcccgcg caacggcggg
    19681 catagtctgt cagctcatga taattccact gataacgcat gtccgttacc gccagcgcct
    19741 gcggctgttg cagagcgata cggaaaatgc cttcatgcag caagccttcg ggaatggggg
    19801 cgccggtggc gttgacccgt tcgcgtatcg cgcgctggct ggcgggcatc atatctgcga
    19861 acggcttttg ccaggcgctt tcgtcatcgc agagttggtt aatcaactgg cagtaggcgt
    19921 cgaacaatgt ttcgactaac gccggaggga acagcgcgtc gttgctgtcc cattgtagcc
    19981 agacctcgcc gtgatgctcg aacgccagat gatctatcca gacctgcggc gtttgcgaga
    20041 tgccccattc cggctcgccc aacggcgatt ctgcgcggct gctgtagagg gaacgcccca
    20101 gattgctggt aaataccacc ggggcgccgt gggggtagcg ctgctggcgt ttgagttcac
    20161 ggagtaattc gacgccggac cagtggcgat gctcccagtc ctccgtaaac gtgagctggt
    20221 ttttacgcgc caggttgctg acggtatcgc catcgcaggc ggtatccagc agaagaatat
    20281 tggtgaagtc ggcaagcatc gcgccaaccg ccggttgcag cggctggcgg tcgaataagg
    20341 tgatgttaag cagcagacgc gtcaggccgc cccagcgagc cagcacggca gaaaaacagg
    20401 tcgccagtgc cattgtcggc gtcacgccat actcgccggc ccggttgcta aaggcgtgcc
    20461 agcgtgttgc cgggacaatc atgcggcgtc gggtattacg gacttcacgt agcgtggcgg
    20521 gttcgcaggc cagcggcaag acgggcgcgg ggggaagcgt cgatgctttc gccagccagt
    20581 aagcgcgcgc gacgtctctc agtggttgat tgatcttctg ctggtgcagc aaatacgagc
    20641 ggaaatcata gcgggtgtcg atagccggca gcgattctcc ggccagcagg gcgttaagct
    20701 catcgaagaa aagcgtaaag ctggaggcat ccataatcag caggtcaata ttgacatgga
    20761 ggcggtggcg attgtccggc aagagcgtca gctgaaaatc aaacgtttcg ccgatttcca
    20821 cgcgtaaaag acggtggctc aggcgctggc gcagtgcgtc cagataggcc tggcggcttt
    20881 cagcgtcgtt atggcgtaaa tcatgaacgg tgacgccgtt ccagtaaggt tgcggtagcc
    20941 agacctgctg cccgtcgggg cgaaaggcga tatgcagcat tgggtggcgt tgcagcaagg
    21001 tcgtgatggc ctgctccagc tgcgacgccg tcagacaatg gccttcaaac tcctgataca
    21061 ggtggcaacc cacgccgcca agcgtctgcc ccggcatgcg gcccgtcagg taggcgtgct
    21121 gtactggcgt caatgggaag ggggtacttt cggtcatgtt cggccaggat gattcgtcgg
    21181 gcggcgtttc ttcttccgcg ttctccggcg accggctgag cattaactgg ttccatgccg
    21241 ccagcgtggg ggcggcatac agctcgcgaa gggtaaggcg gtagccattt ttacgaaacc
    21301 agtgtaacca tctcatcaat cttatggaat ccagaccggc ctggatcagg ttgctctctt
    21361 catgtaactg ctgcggcgtt aaattcagtt cctgtatgag ccgctcgcgt aattgttggt
    21421 aatcagccgc gtggcggttg tccggtaaca gcgaatcctg agatggtgcg ccagaaatca
    21481 ttcttcctcc tgatggcacg tcttaccgaa aagcattact catatgagaa taataattat
    21541 taacaattgg atgctacccg aaacgggttt atttatgccc gtttcgggtt ggtaacaggc
    21601 ggaaagaaca gaggcccgga tggggtatcc gggcctctgt cagggaggag tttagggggg
    21661 cgcgaccccg gttacatccc gcgtttaaag gtcgaaggag ttacgccaaa ctgtttctgg
    21721 aaggcggcag agaaatggct ggcgttggca tagcccagat cggccgccac cgtcatgacg
    21781 gaggtattgc cgttggccag gcgtcttctg gcttcctgca ttcgttcagc ctgaaacagg
    21841 ccatagacgc tgttgttgaa cagaacgcgg aagcctcgct taattttgag aacgctcaga
    21901 ccggactctc tggccagctc cgcgacgccc ggcgcttgcg tcagatcagc cagcagcaga
    21961 tccttcgccc gcagcagttt ctggcgttcc atcgggttga agtggcagga gaggcaactg
    22021 gcgtcttcgc gctgttcatc cagcaccaga ctgagcatga ccagactttg cccctgcaac
    22081 catagcgtcg acggcttttg ttcatgattg aagccgtcgt gcatgcgcat cagcgcgtga
    22141 cgcaaggcct gagccgttaa gtgggtttca gcattacaac gctgctgctg gcaacagcag
    22201 tgggagtcga tcttattctt aatgaccgcg ctaatatccg gcacccacag cgcgagggtt
    22261 tcagggctga aggagagggt aatgctctcg aatcggccac aataggaggc gctgccgcga
    22321 cagtctggcg tatgggtaat gtaatggcga ttgcgcgcaa gctcccagtc ggtgtggcgc
    22381 cggagtccct gaatcgcaaa gcgcgatgtg ccttccagga tgcacgagaa gttaatcctg
    22441 cccagatcgt cgtgtagctg caacgtctca ggccgcgcaa aatggcccct ccagagcaaa
    22501 atatccatac cttcctgcaa cgaacagcgc agcagttcac actgagcagg aatattgcca
    22561 tcgtttgcag gtttcccgac caccaactgg tgaatagaga tttcagattg cgtttgcggt
    22621 gactccgtca tgacctggtt atctccctgt ggcttagacc aatgcgggta taaatagaac
    22681 cgaatggggt agatgagtta ttgataatgg ttattattca cattaattct cagaacgcca
    22741 ccctgaattg tcgtttttgg cgtccgtaaa aatgcatcag gaaattcagt tactcccatg
    22801 tcatctcaat catccaatac tgaatcgctg tcccgatttc ctctctggca ggtgattaca
    22861 ccggttcgca ggaaggttat tttagctatg gcgctggcgg ggctggcggc attaaccagc
    22921 ctgggggcgc tcctttttct ggtgtggagc ctgcgtgaca ttcgcgcgac gcctgacgct
    22981 attccggcct ggccgctggg tggcgtgatc ggctgcgtgg tcttaacttt tgttctgcgc
    23041 ttacaggcgt tcaacacctc tcattacgcg gcttttcatc tggagaacat tctgcgcagc
    23101 aggttagccc gtaaagcatt gcagcttccg cctggcgtgt tacagcaaat gggcagcggg
    23161 tcagtggcga aagtgatgct ggatgacgtg aagtcgttac atatttttgt ggccgacagc
    23221 acgccgctct atgcccgcgc gatcatcatg ccgctggcga caatcgttat cttgttctgg
    23281 ctggactggc ggctggcaat cgcgacgctg ggggtactgg cgtttggatc ggttgttctc
    23341 gtgctcgccc gccagcgttc ggaagatatg gctcagcgtt atcataaggc gcgcgagcag
    23401 gttagcgcgg cggttattga gttcgtgcag gccatgcccg tggtgagaac gtttgatagc
    23461 ggcagcacca gttttttgcg ctatcaacgc gcccttgaag agtgggtcga tgtgctccaa
    23521 acctggtatc gcaaagccgg tttttcagcg cgtttttcct tctcgattct gaatcctctc
    23581 ccgaccctgt ttgtcctgat ctggtcggga tacggcctgt tgcactatgg cagtttcgat
    23641 tttatcgcgt gggtggccgt tttactgctg ggcagcggaa tggccgaagc cgtaatgcca
    23701 atgatgatgc tcaataacct ggtcgcgcaa acgcgtttaa gcattcagcg tatttatcag
    23761 gttctcgcga tgccggagtt atcgctgccg cagtctgagc agcagccgca agaggcgagc
    23821 attacctttg agcaggtgag ctttcattat ccgcaagcgc gtactggcgc cgcgttgcag
    23881 gaggtgagct ttcatgtgcc tgccgggcaa attgtggcgc tggtcgggcc aagcggcgcc
    23941 ggaaaaagca ccgtggcgcg cttgctgctg cgttacgccg acccggacaa aggccatatc
    24001 cgtattggcg gcgtggatct gcgtgatatg cagacggaca ccctgatgaa gcaactctcg
    24061 tttgtgtttc aggacaactt cctttttgtc gacacgatag ccaataacat tcgtctgggc
    24121 gcgccggata cgccgctgga ggcggtaata gcggcggcca gagtggcgca ggcccatgat
    24181 tttattagcg ctctgccaga aggttacaac actcgagtcg gggaacgtgg ggtatttctc
    24241 tccggcggcc agcggcagcg cattactatc gcccgggcgc ttttgcagga tcgccccatc
    24301 ctggtgctcg atgaggcgac ggcgtttgct gacccggaaa acgaagcggc gcttatcaag
    24361 gcgctcgcgg cggccatgcg tggccggacg gtcatcatgg tcgcgcatcg tctctcaatg
    24421 gtgactcagg ccgatgtgat attgctgttt tccgacggac agctcaggga aatggggaac
    24481 catacgcaac tgttggcgca gggcggcctg tatcaacggc tctggcaaca ctatcagcag
    24541 gcgcagcatt gggtgccggg tggaacacag gaagaggtgg tggaaaatga aagacaataa
    24601 tcctgcggat aacctggcct ggcgcgtcat ctggcgccag cttatctcca gcgttggcag
    24661 tcaggccagg atgctgcgac gcagtatgct ggcgctgttg ctggcggcat tcatgcaggg
    24721 gatcgccttt gcctgtcttt atccgatcat tgatgcgctg ttacggggag acgcgccgca
    24781 acttcttaac tgggctatgg ccttcagcgt cgccgcaatt gtgacgctgg tgctacgctg
    24841 gtatggcctg ggctttgaat accgtggtca tctggcgcag gctacccatg agttgcgcct
    24901 gcgacttggc gagcagttac gccgcgtgcc gctggagaag ctccagcgcg gcagggcggg
    24961 tgaaatgaac gccttgctgc tgggcagcgt ggatgaaaac ctcaattatg ttattgcgat
    25021 agccaatatt ttgctgctca ccattgtcac gccgctgacg gcgtcgctgg cgacattgtg
    25081 gatagactgg cggctggggc tggtgatgtt gctgatcttc cctctgctgg tgccgtttta
    25141 ttactggcgc cgcccggcga tgcggcgaca aatgcagacg ctgggggaag cgcaccagcg
    25201 tctgagcggc gatatcgttg aatttgctca ggggatgatg gtattgcgca cctgcggcag
    25261 cgatgccgat aaaagccggg cgctgctggc gcatttcaat gcgctggaaa acttacagac
    25321 ccgcactcac cgtcagggcg ctggcgcgac gatgctgatc gccagcgtcg tggagttggg
    25381 cctacaggtg gtggtgttat ccgggatcgt ctgggtagtg acgggcactc tgaacctcgc
    25441 ctttttgatt gccgccgtcg cgatgattat gcgcttcgca gaaccgatgg cgatgtttat
    25501 cagctacacc tcggttgtgg aactgatcgc cagcgccctg caacggattg agcagtttat
    25561 ggcgatagca ccgcttcccg tcgcagagca aagcgagatg ccggaacgtt acgatatccg
    25621 ctttgacaac gtcagctatc gctacgaaga aggcgacggc cacgcgctta atcatgtttc
    25681 tttgacgttc ccggcagcca gtatgagcgc gctggtgggt gcctccggcg caggcaaaac
    25741 taccgtcacc aaactgttaa tgcgctatgc cgatccgcag caagggcaga tttctattgg
    25801 cggcgtcgat attcgccgcc tgacgccgga acagctcaat agcctgattt ctgttgtttt
    25861 ccaggatgtc tggctgtttg atgacacgct gctggcgaat atccgtatcg cgtgcccaca
    25921 ggcgacgcgg caggaggtag aagaggccgc ccgcgcggcg cagtgccttg agtttatttc
    25981 ccgtcttccg caaggctggc tgacgccaat gggtgagatg ggcggccagc tatcgggcgg
    26041 cgagcgccag cggatttcca ttgccagagc gttattgaaa aacgcgccag tcgtcattct
    26101 cgatgaaccg actgccgcgc tggatattga aagcgagctg gcggtgcaaa aagcgatcga
    26161 taacctggtt tacaaccgga cggtgattat catcgctcac cgtttatcca ccatcgccgg
    26221 ggccggaaac attctggtga tggaagaggg acaggtggtt gagcagggta ctcatgcgca
    26281 attgctctca catcatgggc gttatcaggc gctgtggcag gcgcaaatgg ccgcgcgcgt
    26341 gtggcgcgac gacggggttt ccgcgtctgg agagtgggtg catgagtgat gttcagtcga
    26401 atgtgaaacc gctgacgttg acgaccgggc gggtgatttt tgctattgcc ggcgtctatg
    26461 tgacgcagag tctggtatcg gcgctgtcta tgcagtcctt acccgcgctg gtgcgcgctg
    26521 ctggcggttc gctggcgctt gccggtgcga caaccctgtt tatgctgccc tgggcgctga
    26581 agtttatttg ggcgccgtgg atcgagcgct ggcggcttcc gcccggtagc caggaacgcc
    26641 gttcccgcat gttaatcctg cgtggtcagg tcgcgctagc ggcgatcctg actattgccg
    26701 cagcgattgg ctggtttagg cgagaagggg gattccccga tacgcaaatc gtcgcgttat
    26761 ttgttctgtt tatggtggca ggcacggtcg cctccaccat tgatatcgcc agcgacggct
    26821 tttgtgtcga tcaactgacc cgcacgggtt acggctgggg aaacagcgtg caggtcggcg
    26881 gcagctatct gggaatgatg tgcggcggcg gggtgttcat gatgctgtcg gcggcatccg
    26941 gctggcctgt cgccatgctg atgatggcgg tgctgattat ggcgctgtca ctcccgctgt
    27001 ggcgcattac ggagccgacg cgaacagcga ctatcctgca tgttccggcg ttaggttatg
    27061 cgctaaggag gaagcaggcg cgcctgggct tactgctggt attgatgctg aattcaggca
    27121 tgcggtttgt gctgcctctt ctggcaccgc tgttgttgga tcatgggttg agcatgtccg
    27181 cattgggcgc gttgttcagc ggcggcaata ttgcagcggg catagcagga acgctggctg
    27241 gcggattgct gatgaaatac acctcacccg gcagagcgct gttgacggct tatggcgtcc
    27301 aggggatcgc gctgctggcg gtggtgatga cgctcatgat ggcgccgggt catctgctgc
    27361 tgcagattct ccagtgtctg gtcattgtcc agtccatttc gctggcctgc gcgctggtct
    27421 gtctttacgc cacgctgatg tcgctttcat cgcctttgca ggccggtgtc gacttcaccc
    27481 tctttcaatg tactgatgcg gcaatcgcca ttctggcagg cgttatcggt ggcgttgttg
    27541 ctcaacattt tggctatgcg gcctgcttcc tgtttgccgg ggcattcacg ttgctggcgg
    27601 cgtgggttgc ttatatccgg ctgcattcgg caagagaact gatgacaagc gcaattgatt
    27661 gatccgcgaa taacacagag aggtaaggga tgaaaatcag tgaatttcta catctggcgt
    27721 taccagagga acaatggcta ccgacgattt ctggcgtttt acgccagttc gcagaagaag
    27781 agtgttatgt ctatgagcgt caaccctgtt ggtatttagg caaagggtgc caggcacggc
    27841 tgcacattaa tgccgacgga acgcaggcga catttattga tgatgccggg gagcaaaaat
    27901 gggcggtgga ttccattgcc gactgcgcgc gtcgttttat ggcgcatcct caggtgaaag
    27961 gacgtcgggt atatggacag gttgggttca actttgcggc gcatgcgcgg gggattgcct
    28021 ttaacgccgg ggagtggccg ctgctgacgt taaccgttcc ccgtgaagaa cttatttttg
    28081 aaaagggaaa tgtcaccgtt tatgcggact ccgccgacgg gtgccgacgt ttgtgcgagt
    28141 gggtaaaaga ggccggtaca acgacgcaga acgcaccact ggcggtggat accgccctca
    28201 atggtgaggc atataaacaa caggttgcgc gcgccgttgc ggagatccgc cgtggcgagt
    28261 atgtcaaagt gattgtctcg cgcgccattc ccctgccatc gcggattgat atgcccgcca
    28321 cgctgttata cgggcggcag gcaaacacgc ctgtgcgctc gtttatgttc cgtcaggaag
    28381 gacgcgaagc gctgggcttt agcccggaac tggtgatgtc agtgatgggc aataaagtgg
    28441 tcactgaacc gcttgcgggc acccgcgatc gcatgggaaa cccggagcat aataaggcga
    28501 aagaggcaga actgctgcac gacagtaaag aggtgcttga gcatatcctt tctgtcaaag
    28561 aagctattgc tgaactggag gctgtttgcc agccgggcag cgtggtggtt gaagatttaa
    28621 tgtcggttcg ccagcgcggc agcgttcagc atctggggtc tggcgtgagc ggtcagcttt
    28681 cggaaaacaa ggatgcctgg gatgcgttta ccgtgctgtt tccgtcgatt accgcctcag
    28741 gtatccctaa aaatgctgct ctgaacgcga ttatgcaaat tgagaagacg ccgcgagagc
    28801 tttattccgg cgcaatcctg ctgctggacg atacgcgctt tgatgcggcg ctagttctgc
    28861 gttccgtatt tcaggatagc cagcgctgct ggatacaggc gggggcggga atcatcgcgc
    28921 aatctacgcc ggaacgcgaa ctgacagaaa cccgggagaa attagcgagc attgcgccct
    28981 atttaatggt gtaggagtaa ctgttttcta acctcacaac cagactgtcc aaagacaatt
    29041 gagtcgctct ttgattcctc tgtgactcta ttgtcttctt ttgggataaa ataggtgttt
    29101 ttcgtcatga agttgataaa agtctatata gaaccacctc ctgtggaggt ggtggtaatt
    29161 tgccagagtg agggattcgt tagtatacta tgggataaac ttatttttac caattgtata
    29221 aggcggcgca taagattctc ggcatgcttc cagataatcc gaccaccact gcatcatcgc
    29281 tttgcgggct tctaggtgct ctgccttatg aatataagcc atgcgcacgg tattgcgctc
    29341 ctgatgactc atctggcgtt ctactgcgtc ctttgcccat aaacccgatt ccatcagcgc
    29401 actgcatgcc attgcccgga agccgtgacc gcagatatcc tttttcgtgt cgtaacccat
    29461 cacccgcagt gccttattga ccgtgttttc acacattggc ttatacgggt tatggtcgcc
    29521 ggggaagatc agttcattat taccggtgat atccttaatc tgtttcagaa tggcgatgga
    29581 ctgttctgag agggggacga tatgcggcat tcgcattttt gccccgcggc cggaataacg
    29641 cacgccaata atgggttctc gcgtcgcggg tatcgtccag actcggtttg tgaaatcaat
    29701 ctctgaccag cgggcgaaac gcagttcact ggagcgaatg aacacatgca gcatcagcag
    29761 aacggcatgc cgggtcagtt cacggccctg atgatatgcc ccaatacgtt caagcagttc
    29821 aggcagccgc tccagcggca gggcaggata gtgccgtctg acaggaggtg tggtcacgcc
    29881 gccaaggttt gctgcagggt tcgtatcgat taactcctga tggaccgcat ggcgcattat
    29941 gttactcagg tgctgccgtg tgcgggacgc aacctccagc tttgagatac cagtggcggg
    30001 agccacccgg cttgaccagc agatacagac cgtgagaatc ggagacttta aagggtttat
    30061 cagaaggctt gagggtgcgg atttttgcgt cggtaaggaa catatggggg tcactccatt
    30121 atcgaactaa cctgatccca gatttgacca ccaatttttc ccgatgcata gggtaaaatc
    30181 aaaatgcacc gagaagagtt ttcacactaa cctgttgaat caacatcaga taaagatttg
    30241 caaggatgca tgaaaacaga aaattggctc ctctgactgg actcgaacca gtgacatacg
    30301 gattaacagt ccgccgttct accgactgaa ctacagagga atcgtgtgaa cggagcgcat
    30361 attacttagc ggtaccttgt ctgtcaacac taaatttcat aagccatttc aattggttaa
    30421 ttaatctgca aagttgttaa ttaatgaaca ttcgtcgccg aaaacgagtc tgtatcatta
    30481 gcgtgatgca gtctctgcaa aggatcttgt tgataaaatt gacagaaacg ttgccacaat
    30541 gaagggaaac gaggagcaaa aagttctggg gcgctaaaga aatattcaga aagtacggca
    30601 aaacattcag caggatcact ggcagcataa gcatcaatgc tcgctgcatt ctcaccaact
    30661 aattcgattt cttcctgaat gttgttcatt gcagcatgaa gatcgtgttc ccagccagca
    30721 acctcacgca acgaaataaa gggaactccg ctggcgcgat cgccgttacg ggtgtccagc
    30781 ttatgagcga cttcatgaat aatcaggtta aaaccagatg catcaaaaga atcttgtata
    30841 tccaaccagt tcaaaacgat aggcccttgc tgccagctct gacctgactg aacaatacgt
    30901 tggttatgca ccagaccgat atcgtcttcc cattcatcat cgaccacaaa tggcgcagga
    30961 taaattaaga cttcatgaaa accatccagc cattccagtc ctaactccag aacgggtagg
    31021 caaaatagaa gtgctatccg gcagcttctt aatgaatcca gttcaaagcc ctgtaaagga
    31081 acaagccgct tttgctgtaa aaaacgttcg gcaagagtga ctaatttgct ttgttcctgt
    31141 tccgtcagac ccgttaaaag ggggatcgat agtgcttcct gccagggaag ggcagtttga
    31201 tgtgctgatt cttgtacttt ccagggccac ttaatcatcg ttttgctcgc aaactcgtca
    31261 cttgaacaaa attgcacgga cagggactgt taaaatgcca aatttcctgg catcatggca
    31321 accatctgaa cggagagatg ccggagcggc tgaacggacc ggtctcgaaa accggagtag
    31381 gggcaactct accgggggtt caaatccccc tctctccgcc actttatcaa tgacttatct
    31441 cctgacttcc cgccttgctt ttcctaaaca gaacaatcgt agaatattct tgaagggtta
    31501 gatcgtcact gttttctgtt cgatactgtg acattcagca cttgattcgc tatggagctg
    31561 acaggaaggt ttcgagcgaa aatctacagt tattcagtcg ttttcttatc ggtcaccatt
    31621 attcttttag acattgatcc tatgaaactg ccgcaaagtt gctggcggga actgaagttg
    31681 cgtagagaag gggtcaataa ttgagtcaaa aaaggaacgc ggccgtcaga ttaaagccgc
    31741 gggacaaagt ccataatttc caagagttgt tgaatttccc agcttttttg aaagcctttg
    31801 tatcatacta acaagtaaaa aaggtttaaa caccaaaatt aacctatatt actggttagc
    31861 tttaccatga cagatgactt gctcctggaa acatttgcgc tgccagtctg tatggctgag
    31921 ttgtgtattt attgttcgcc tttttcgcat ttttttctac agacataata aaaaagacac
    31981 gactcaagta tgagcgtgtc tgagtttgtc ttacttattt gtcttggcaa attattgttg
    32041 ttactaccag gcactgttct gttaaataac tgaagtcagc gcaaaaaaat caatgagaca
    32101 tcatctcctc gaccacttct tcgctactca actgataggg ataatacgtt ggccagtttt
    32161 ccatttcatt caataaagac tgttgggaat cattacccat aaaaatgtga aaatgagatg
    32221 attttcgtgg tgcaattata tgatcgctaa attgtatata tttaggggct ttactttcag
    32281 gatctttaca ttcgaataag taacgaacgc ctttcttgcc tgatttatag gtgagtattt
    32341 tgtatccatc gtaatcatat ttacaggatg ttgtttcatt atttctatgg aattcgacaa
    32401 tgccgtcctc aatgccaatc atctcgatgt ctgtcacata acctttgcga taataatctt
    32461 taatttcagc aaatgttttg gttttatctg catccgcttt cttctgaaag atggggtcaa
    32521 gtttgccact ttgcagtaaa ggataaacgg attgccagac tccatcccag tcactgagcg
    32581 ttcggttttg tacattggca tcatcaaaaa caccattagc agctttttgt tcgacctctg
    32641 ttaagggttt gccgtgtgag tgatgaccat gcgaaaaggc aggagcgcta acaataaaga
    32701 cacctaaagc aacagccagt ttatgaagac gaatcgccaa aacagtttcc
//
LOCUS       DAZGJW010000043        32630 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZGJW010000043 DAZGJW010000000
VERSION     DAZGJW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.043"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..177
                     /locus_tag="ACHKIK_002010"
     CDS             <1..177
                     /locus_tag="ACHKIK_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO9083185.1"
                     /translation="RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     SLKQEPTEMIQATA"
     gene            221..328
                     /locus_tag="ACHKIK_002011"
                     /pseudo
     CDS             221..328
                     /locus_tag="ACHKIK_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(312..950)
                     /gene="ytfB"
                     /locus_tag="ACHKIK_002012"
     CDS             complement(312..950)
                     /gene="ytfB"
                     /locus_tag="ACHKIK_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO9083186.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1169..1789
                     /gene="fklB"
                     /locus_tag="ACHKIK_002013"
     CDS             1169..1789
                     /gene="fklB"
                     /locus_tag="ACHKIK_002013"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO9083187.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2098..3501
                     /gene="cycA"
                     /locus_tag="ACHKIK_002014"
                     /gene_synonym="dagA"
     CDS             2098..3501
                     /gene="cycA"
                     /locus_tag="ACHKIK_002014"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO9083188.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRMAK"
     gene            complement(3543..3641)
                     /locus_tag="ACHKIK_002015"
                     /pseudo
     CDS             complement(3543..3641)
                     /locus_tag="ACHKIK_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3768..4196
                     /locus_tag="ACHKIK_002016"
                     /pseudo
     CDS             3768..4196
                     /locus_tag="ACHKIK_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4301..5368
                     /locus_tag="ACHKIK_002017"
     CDS             4301..5368
                     /locus_tag="ACHKIK_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083189.1"
                     /translation="MAILHPQECWLLERIMSPEYYRRRFEGWQEFVELCERQVAEWSK
                     TMPLDVRRRPLCEQIDVVWGGRVLPNIRSTLKSVQYDFIQLQQGDLRVLQSGGNISSD
                     MKGLIDYPSDWMSLAAQKQYDRLKWRGAHYNNLIRRTSGGYWYDGELTYYYEESLHGP
                     LALPMQLPLYELDSSVYLREDDSVTVAGLYLPDIPDASAQLLYRSEHIPEAWQGRVRT
                     KYVNEAGIQEYYWESGAWAKCNWKRIRRVANRFINVPPEGFFPQGMPEELYNWPQREA
                     QYVTDRQRIAAFSGEACPHSGEWSIFVEGRQATVTLEQGEQMPEWTDRKMEGEYKRGE
                     KFHVLWSLMNRHDGGSVWVEA"
     gene            complement(5615..6277)
                     /gene="ytfE"
                     /locus_tag="ACHKIK_002018"
     CDS             complement(5615..6277)
                     /gene="ytfE"
                     /locus_tag="ACHKIK_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO9083190.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6385..7350)
                     /gene="ytfF"
                     /locus_tag="ACHKIK_002019"
     CDS             complement(6385..7350)
                     /gene="ytfF"
                     /locus_tag="ACHKIK_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO9083191.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(7458..8318)
                     /gene="qorB"
                     /locus_tag="ACHKIK_002020"
     CDS             complement(7458..8318)
                     /gene="qorB"
                     /locus_tag="ACHKIK_002020"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO9083192.1"
                     /translation="MIAITGATGQLGHYVIESLKKTVPVSQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            8407..8787
                     /gene="ytfH"
                     /locus_tag="ACHKIK_002021"
     CDS             8407..8787
                     /gene="ytfH"
                     /locus_tag="ACHKIK_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO9083193.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(8916..10859)
                     /gene="cpdB"
                     /locus_tag="ACHKIK_002022"
     CDS             complement(8916..10859)
                     /gene="cpdB"
                     /locus_tag="ACHKIK_002022"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO9083194.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            11049..11789
                     /gene="cysQ"
                     /locus_tag="ACHKIK_002023"
     CDS             11049..11789
                     /gene="cysQ"
                     /locus_tag="ACHKIK_002023"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO9083195.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(11779..12336)
                     /gene="ytfJ"
                     /locus_tag="ACHKIK_002024"
     CDS             complement(11779..12336)
                     /gene="ytfJ"
                     /locus_tag="ACHKIK_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO9083196.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            12661..12867
                     /gene="ytfK"
                     /locus_tag="ACHKIK_002025"
     CDS             12661..12867
                     /gene="ytfK"
                     /locus_tag="ACHKIK_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO9083197.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(12929..14272)
                     /gene="paeA"
                     /locus_tag="ACHKIK_002026"
     CDS             complement(12929..14272)
                     /gene="paeA"
                     /locus_tag="ACHKIK_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO9083198.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14595..15233)
                     /gene="msrA"
                     /locus_tag="ACHKIK_002027"
     CDS             complement(14595..15233)
                     /gene="msrA"
                     /locus_tag="ACHKIK_002027"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO9083199.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            15439..17172
                     /gene="tamA"
                     /locus_tag="ACHKIK_002028"
     CDS             15439..17172
                     /gene="tamA"
                     /locus_tag="ACHKIK_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO9083200.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            17169..20948
                     /gene="tamB"
                     /locus_tag="ACHKIK_002029"
     CDS             17169..20948
                     /gene="tamB"
                     /locus_tag="ACHKIK_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO9083201.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTVQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     NRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            20951..21292
                     /gene="ytfP"
                     /locus_tag="ACHKIK_002030"
     CDS             20951..21292
                     /gene="ytfP"
                     /locus_tag="ACHKIK_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO9083202.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            21504..21755
                     /gene="chpS"
                     /locus_tag="ACHKIK_002031"
     CDS             21504..21755
                     /gene="chpS"
                     /locus_tag="ACHKIK_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFO9083203.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            21749..22099
                     /gene="chpB"
                     /locus_tag="ACHKIK_002032"
     CDS             21749..22099
                     /gene="chpB"
                     /locus_tag="ACHKIK_002032"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFO9083204.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(22179..22709)
                     /gene="ppa"
                     /locus_tag="ACHKIK_002033"
     CDS             complement(22179..22709)
                     /gene="ppa"
                     /locus_tag="ACHKIK_002033"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO9083205.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            23019..23975
                     /gene="ytfQ"
                     /locus_tag="ACHKIK_002034"
     CDS             23019..23975
                     /gene="ytfQ"
                     /locus_tag="ACHKIK_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO9083206.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            24115..25617
                     /gene="ytfR"
                     /locus_tag="ACHKIK_002035"
     CDS             24115..25617
                     /gene="ytfR"
                     /locus_tag="ACHKIK_002035"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO9083207.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            25631..26653
                     /gene="ytfT"
                     /locus_tag="ACHKIK_002036"
     CDS             25631..26653
                     /gene="ytfT"
                     /locus_tag="ACHKIK_002036"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO9083208.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            26640..27635
                     /gene="yjfF"
                     /locus_tag="ACHKIK_002037"
     CDS             26640..27635
                     /gene="yjfF"
                     /locus_tag="ACHKIK_002037"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO9083209.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(27668..28666)
                     /gene="fbp"
                     /locus_tag="ACHKIK_002038"
     CDS             complement(27668..28666)
                     /gene="fbp"
                     /locus_tag="ACHKIK_002038"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO9083210.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            28842..30215
                     /gene="mpl"
                     /locus_tag="ACHKIK_002039"
     CDS             28842..30215
                     /gene="mpl"
                     /locus_tag="ACHKIK_002039"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO9083211.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(30371..30922)
                     /gene="yjgA"
                     /locus_tag="ACHKIK_002040"
     CDS             complement(30371..30922)
                     /gene="yjgA"
                     /locus_tag="ACHKIK_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO9083212.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWHDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            31016..32368
                     /gene="pmbA"
                     /locus_tag="ACHKIK_002041"
     CDS             31016..32368
                     /gene="pmbA"
                     /locus_tag="ACHKIK_002041"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO9083213.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            32551..>32630
                     /locus_tag="ACHKIK_002042"
     CDS             32551..>32630
                     /locus_tag="ACHKIK_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="electron transport protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO9083214.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLE"
BASE COUNT         7729 a         8134 c         8909 g         7858 t
ORIGIN      
        1 cgcctgtcac aaagtaaatt cagatgccag gtatgtggat atacagcgaa cgccgatgta
       61 aacggcgctc gtaacatttt agcggcgggg cacgccgttc ttgcctgtgg agagatggtg
      121 cagtcaggcc gctcgttgaa gcaggaaccc accgaaatga ttcaggcgac agcctgaacg
      181 aagcagggat ccacgtcctt cagggcgtgg aggatgtcaa catatcagta tggtttcccc
      241 ccaggcactg gaagccagac gtaaggtcat caaagagaca atccttagct ggcttttggt
      301 tgacccttca tctaccgcgc acgaataaaa ctgccatccg gctggcgggt aaacaggacc
      361 tgttgattat tccccgtatc aatggttaag cccgtcacca cgccgctggc gttctggcgg
      421 attttcacca tttgcccgtt ttgcaaatta ctcagcggct tacccgcgcc ttctacctgc
      481 gccattgcat agacatcggt agcgggcagt ccgtggtcgc ggaacagctg cgccatcgtt
      541 ttaccgggtt cgacacggta agagcgccat tgattatcaa ttccgctatc tggctggaat
      601 ggttgcgttt gggtagtttg cggctgctct tctggctggc cttcctgtat aggttctgga
      661 gcgaccggag ccacctgatc aggatcattt tgtggggtga ctaattgcgc ccgcagctgt
      721 tcttctgtcg gcggttgtga ttgcgactga atatctaact gcgcttcacg cgtcaccacc
      781 ggcgcgttgg gcgtatcatc agatggaagc agaaatccga ccaccaatac aatggcggca
      841 ataatgatcc cccgacgatg catcggcggc agcgggtcca tgaagcgaaa attgtccggc
      901 gcgtgccaga ctttctccag ggttggtttt agttcaaagc gcccgggcat ggtttcctcc
      961 tgctccgcgt cttgttcctc aatcatagcc tatgaataag ctaacgctat gatgtccgtg
     1021 gtaaacccgc ctttattata ttcatacggg atattgctat tgtttctttt tccccgggat
     1081 ttgtcatcat tcctgcgaca aagttttacc caaagaagtg tggctgatat gctgcccgct
     1141 acttttatac cctaagaaag gaaatacgat gaccacccca acttttgaca ccatcgaagc
     1201 gcaagcaagc tacggcattg gtttgcaggt agggcaacaa ctgagtgaat ctggcctgga
     1261 agggctgctg ccagaagcac tggttgcagg tattgccgat gcgctggaag gcaaacatcc
     1321 ggctgttccg gttgatgtgg tgcatcgcgc gctgcgtgaa atccacgagc gcgccgatgc
     1381 cgttcgtcgt cagcgtttcc aggcgatggc tgctgaaggt gtgaaatacc tggaagaaaa
     1441 cgccaaaaaa gaaggtgtga atagcaccga atctggcctg caattccgcg tgatcaacca
     1501 gggtgaaggc gcaattccgg cacgtaccga ccgcgttcgt gttcattaca ccggtaaact
     1561 gatcgacggc accgtgtttg acagctccgt tgctcgtggt gaacccgctg aatttccggt
     1621 taatggcgtg atccctggct ggattgaagc actgactctg atgccggtag gttctaaatg
     1681 ggaactgact atcccgcagg aactggcata tggcgagcgc ggcgcaggcg catccatccc
     1741 tccgttcagc accctggtgt ttgaagtcga actgctggaa atcctctaag cagcgcattc
     1801 tgttcccctc gaaccagagg ggagcaggca ttcagcaata aacccttcag tttgccaaac
     1861 ggcgctattt tgtgttgcaa agaccccgta agcgtgtatt tttgtgagct gtttcgcgtt
     1921 atcaccgtga tatgacactc actttaaaca taaaattaac attagatcta aatcttagta
     1981 ttcatcccgc gtattgttac ctaatatcga tgagtcccga tacagattcg tcgtatcata
     2041 ggctgactaa aggccgtaga gcctgaacaa cacagacagg tacaggaaga aaaaaacatg
     2101 gtagatcagg taaaagtcgt tgccgatgat caggctccgg ctgaacagtc gctacggcgc
     2161 aatctcacaa accgacatat tcagcttatt gccattggcg gtgccattgg tacggggttg
     2221 tttatggggt ctggcaaaac gattagcctt gccgggccgt cgatcatttt cgtttatatg
     2281 atcatcggtt ttatgctctt tttcgtgatg cgggcaatgg gggaattgct gctttcgaat
     2341 ctggaataca aatcttttag tgacttcgct tccgatttac tcgggccgtg ggcaggatat
     2401 ttcaccggct ggacttactg gttctgctgg gttgtaaccg gtatggcaga cgtggtggcg
     2461 atcacggctt atgctcagtt ctggttcccc gatctctccg actgggtcgc ctcgctggcg
     2521 gtgatagtgc tgctgctgac gctcaatctc gccaccgtga aaatgttcgg tgagatggag
     2581 ttctggtttg cgatgatcaa aatcgtcgcc attgtgtcgc tgattgtcgt cggcctggtt
     2641 atggtggcga tgcactttca gtcaccgact ggtgtggaag cgtcattcgc gcatttgtgg
     2701 aatgatggcg gctggttccc gaaaggttta agtggcttct ttgccggatt ccagatagcg
     2761 gttttcgctt tcgtagggat tgagctggta ggtacaaccg ctgcggaaac caaagatccg
     2821 gagaaatcac tgccgcgtgc aattaactct attccgatcc gtatcattat gttctacgtc
     2881 ttcgcgctga ttgtaattat gtccgtgacg ccgtggagtt cggtagtccc ggagaaaagc
     2941 ccgtttgttg aactgttcgt gttggtgggt ttgcctgcgg ctgccagcgt gatcaacttt
     3001 gtggtgctga cctctgcggc gtcttccgct aacagcggcg tcttctctac cagccgtatg
     3061 ctgtttggcc tggcccagga aggtgttgca ccgaaagcgt tcgctaaact ctctaagcgc
     3121 gcagtacccg cgaaagggct gaccttctcc tgcatctgtc tgctcggcgg cgtggtgatg
     3181 ctgtatgtga atcccagcgt gattggcgcg ttcacgatga tcacgacggt ttccgcgatt
     3241 ctgtttatgt tcgtctggac gattatcctt tgctcgtacc tggtgtatcg caaacagcgt
     3301 cctcatctgc atgagaagtc gatctacaag atgccgctcg gcaagctgat gtgctgggta
     3361 tgtatggcgt tctttgtgtt cgtgatcgtg ctgctgacac tggaagatga cactcgccag
     3421 gcgctgctgg ttaccccgct gtggtttatc gcgctggggt tgggctggct gtttattggt
     3481 aaaaaacgca tggcgaagta atgccgctaa ggcacctgcg ggtgtctttc aggctgcaaa
     3541 attgcctgat gcgctacgct tatcaggcct acagaacctc tgcaatatat tgagtttgcg
     3601 tgcttttgta ggccgggtaa ggcgtttacg ccgcatccgg catgaacaaa gcacactttg
     3661 tcagcaatct cctttgtctt tatacattcc taaaaatact tcccgtcatc gacagctctc
     3721 aggcgcgggc ttaccgtgga tctttgccac tcattgcaga gttcactatg gctaagcaca
     3781 gttggttatt aatcgccgca ttacctctct ccatttcacc ttcctggggc gcagattttt
     3841 attaccgcca gcaggagaaa ggcacggttt atgttgccga acagaaggga gaaaaggatg
     3901 agatcctctc cgaactgccg ggcgttaatt tttcccgcct ctggcgtatt gccaatttag
     3961 ccaataacca agaaacccgc ttactgtccg attttaatcc cgataagttc gattgcgatg
     4021 atcggaataa ttgccagcat acctggctca ccgatggacg ctctgttctt tggtctggca
     4081 aagtcctgaa aaatccgccc ggtgaaccta atgtcgatgc cgccagtttt caggcattcg
     4141 gtgctttcgc tgccgataaa cgcagcgtct attttgacgg tcagcgtacc gatgatagat
     4201 gaagtggata tgtatatagc tgtgtctcag ttgcaaacaa atatttatgt accgaaataa
     4261 aagtatcaat agacaattaa ttaataaatc aggagataaa gtggctatat tacatccaca
     4321 ggagtgctgg ttgctggaaa gaataatgtc gccggaatat tatcggcgtc gttttgaggg
     4381 ctggcaggag tttgtcgagc tgtgcgagcg acaggtggca gagtggagca agaccatgcc
     4441 gctggatgtg cgccgtcgtc cgctgtgcga gcagattgac gtcgtctggg gcgggcgagt
     4501 gttgcccaat atccgcagta ctttaaagtc ggtacagtat gactttattc agctacagca
     4561 gggggacctg cgggtactgc aatccggggg caatattagc agtgatatga aaggactaat
     4621 tgattatccg tcggactgga tgtcactcgc cgcgcaaaaa cagtatgaca gactgaaatg
     4681 gcgaggtgca cattataata atttaatcag acgtacctcg ggcggatact ggtatgacgg
     4741 cgaactgact tattattatg aggaaagtct gcacggcccg ctggccctgc cgatgcaatt
     4801 accgttgtat gagctggata gtagcgtata cctgcgtgag gatgactcgg taacggtggc
     4861 tggcctgtat ctgccggata tcccagatgc cagcgcccaa ctgttgtacc ggagtgaaca
     4921 tattcccgag gcttggcagg ggcgggtacg aacgaagtat gtcaacgagg ctgggataca
     4981 agaatattac tgggaaagcg gggcgtgggc aaaatgcaat tggaaacgta tacggcgggt
     5041 ggcaaaccga tttatcaatg tgccgccgga gggatttttc ccacagggca tgccggagga
     5101 gttgtacaac tggccacagc gcgaagcgca gtatgttaca gaccggcagc ggatagcggc
     5161 ctttagcggt gaagcttgtc ctcatagcgg ggagtggtcg atttttgtag aaggtagaca
     5221 agcgacggtg acactggagc agggagagca gatgccagaa tggacggaca ggaaaatgga
     5281 gggggagtat aaaagaggcg agaaattcca tgtgctgtgg tcgctgatga accgccatga
     5341 tggcgggtcg gtgtgggtgg aagcgtagcg gtagctggat gtacacgtaa aaaccaggga
     5401 atgggtgatt ttattctggc ttatacagga gcgatgaata agtaattata ttaatgaaaa
     5461 aaataagtta gtaaccgttg aaggaactta ttattggctg aataatgaat tttcccagtt
     5521 cagtagatac aaaagattgt cccggagtgg tgaataactc aaagcatttg aaaacgcacc
     5581 tgattgtgta aaccaggtgc gttgatcgaa caaattactc acccgccagc gcgcgtggga
     5641 acagtacatt gttttccagg ctgatgtgat ccatcaggtc atcaatcagt tcattaatac
     5701 cgttatacat cgctttccag gtggtgcagg cttccggcgg cggtgtgacg ttattggtgg
     5761 tgtgtttaat cacttccagc agttcgcccg cttcatcgtg ctcgctttcc atcacgctga
     5821 ttggtcccat tgcctggctg cccatgcctt gtttgatcat cgggaagagg atctgctctt
     5881 ctttcatcat gtggctggaa agctcttcat gcagcatagt caggtatttt gtcagccctt
     5941 ttggcacgct cggtttgtcg gcgtgaacgc gctcgacttt agtcgcttgc aggatcagct
     6001 ccggcagctg ctcgcggtga cgatcgtggt agcgcacgat gatatggtca atgatttctg
     6061 ccagcggggc gctacgccag tctttctcaa tcggttgttc agcaagcttt gccagttcag
     6121 cttcaatgac ctcaacatcc agttctttac gtgccgccgc gcgcgccagc gtctgcttac
     6181 cgccacagca gtaatccata tcatatttac gaaacagagc cgaagcgcga gggatagaga
     6241 gcgccagttc acctaaaggt tggtcgcgat aagccatagc tgatacctca ttcttaataa
     6301 gataagatgt attttaaatg catctttaag gcaaaagcta taacccttac gtagcagagg
     6361 cttatttaac tcactgccag cgtgtcaact ttctgagaca gattcagtta aaggtttttc
     6421 tggttttgct ctgaccgcaa tgaccacgcc aatcactaac agcgcgatac cgctcagcgt
     6481 cattagcggc ggcatttgct ggcggagtaa aaaggtgtac agcaaacctg ccagcgtttc
     6541 gaaaacaatc agcggcccga gaatcactgt cggtaatcgc tggctggcga cgttccagca
     6601 gagtgcgcca acccatgagc aaagcacggc tatcgcaacc atcagactaa taaacaccag
     6661 cggacggggg ccaaaaggta aggagaagtc cggcgtttgt atattcagcc agtaacaggc
     6721 gacgagatag ccgatgagag aaaccggcag cgtgaccagc gcctgcgccg tcgcccacat
     6781 catcggatgt ttgtcgggat tttcccgcag ccagcgggcg ttgcgcaggg cataccatgc
     6841 ccagcagacc acggaaacta acgctagcac gatgccagag gtataacgtg cccagtcaaa
     6901 atcggggagt ccgtggttta actcagcaat attcacactc gccaggccga tgccaataca
     6961 aatcagtgcc ggggcgagtt ttccccacgc gagtttgccg tcgcgctggc tataaagcag
     7021 attggcaaag acaggaatga ccaccggcag ggtgccgata atcatcgtgg aaacaggcgc
     7081 gccagtacgt tgaatggcac tggcaaggca gaagtaataa atgaggttgc ccatcatagt
     7141 gagcatcaag gcggtaagcc agtcccgacg cgccaactga cgcagacgca cgcgtcccag
     7201 ccaggcaatg ggcagcgcaa ttaaccctaa cgccagataa cgccccatcg actgcaacat
     7261 cgccgggtat tccggtacga tcaacggccc gacaaaaata agcccccaca tcaaccctgc
     7321 taacagggcg tacagcacgc cgctaatcat tactggcatc cattggtctg tcagaagaaa
     7381 gttcaggaag cagacatgat tgctcccggc atcccgtcat tattagagag gatgcgggag
     7441 gatgccactt taattaacta gttattaaca ttaaaaagat ggcttacgct ttcggctaac
     7501 gttgtcgttg ggcggccaat caatttgcta agcgttttgc tgttatcaaa cagaccacct
     7561 ttcgatgcgc caacgtcaga atccgccagc atatccgcca gtccgtcggg cagtccgacg
     7621 cttttcagtg ccgcggcgaa atcggcttcg ctcagatttt gataggtaac ctgtttgccg
     7681 ctctgtttgg tcagctctgc cgctaactgt gtcaatgtcc aggcactatc gcccgccagt
     7741 tcgtaaacct tgccttcgtg accggcttcg ctaatcacgc gtgccgcagc tgccgcataa
     7801 tctgcccgcg ttgctgaggc gattttgcca tcgcccgccg caccgataaa tacgccgtgt
     7861 tccagtgctg ccggggcgct ggcgaggtag ttttcgctgt accagccgtt gcgcagcagg
     7921 gtgtaaacga tgccagaatc agccaacatt ttctccgtct cgatgtgctc atcggcgagg
     7981 ccgagcgggg aggtatctgc atgtagcagg ctggtataag cgataaattt cacaccagcc
     8041 gcctttgcgg cattaataac attacgatgc tgcggggcac gttgacccac ttcgctggaa
     8101 gagatcagca gtagtttttc cactccctga agtgcagatg tcagtgcggc ttcatcgccg
     8161 tagtcagcct gacgcacggt aatgccttgt gctgccagag cctgggcttt tgccggatta
     8221 cgaacgatag ccactatttg gctgacagga accgttttct tcaaggattc aataacatag
     8281 tgaccaagtt ggccagtggc accagtaata gcgatcatgg gaagtctcca tcgttttgtc
     8341 ttgtgtttaa atacgctaac acctaaactt acttttagta agtacgcaca aaaaggtaag
     8401 tatgaaatga gtcaggttag cctgtcgcaa caactgaaag agggtaatct ctttgcggaa
     8461 cagtgcccgt cgcgcgaggt gttgaaacac gtcaccagcc gttggggggt gttgattctg
     8521 gtggcgctac gcgaaggtac tcatcgcttt agcgacctgc ggcgcaaaat cggcggggtg
     8581 agcgaaaaga tgcttgcgca gtcgttacag gcgttagaac aggatggttt tcttaaccgt
     8641 atcgcgtatc cggtggtgcc gccgcatgtg gaatatagcc tcacgccgct gggcgagcag
     8701 gtgagcgaaa aggttgctgc actggcggac tggattgagt tgaatttgcc cgaggtgttg
     8761 gcggtgcggg atgaacgtgc ggcataactg ccggatgcgc tgagcttatc tggtctacgg
     8821 tgcatggctt gtagggcgga taagatgcgc cagcatcgca tccgccaata aatgccggat
     8881 gcggttgatg atttataggc cgaaaaagaa gtgttttact tactcaaatc cacctgataa
     8941 atcgcaaacc cgatatcatc ggtcgcgact ttattcatcg gatattgccc tttctcttta
     9001 ataaacgctg cggctttatc tgacggagaa gtttcgaaac ggatatccag tttcttatcg
     9061 ccagctatcg gtgctaaacg ccagttgtta tctgccgccg ggtgaatttc ccccgcacgt
     9121 ttcgactcat cagcaatcca cgctgccagc accgagcggt tctcatccgg tgaagcaaaa
     9181 gcgatatggc tgtcgcccgt accggcaaat ttgccgccgt aagcgcgata gttattggtg
     9241 gcaacgagga acatggcgtt cggatcaatc ggcttgccat taaaggtcag gttcttaatc
     9301 ctttccgcat tggcattaat catctggcac tcgccgtcat aacgggcagg ctgggtaaca
     9361 tcaatctgat aattcacacc atcaatcaca tcaaagttat aggtgcggaa accatcccag
     9421 ttgatgagtg attgcggttt cgtgctgtta ggatcaatct ggttaaactg tccggcggag
     9481 cactccagcc actctttcac ctctttaccg ctggctttca ccacaatcag cgtattgggg
     9541 tagagataaa gatcggcggc attacggaag gtcaactggc ctttttccac ctccacatag
     9601 cttgccgggt catttttgcg accaccaact ttaaacggtg cggcagctga aagcaccggc
     9661 agttttgcca gatccggatc gccctgaatg taatgttcga catacgcttt ttgcgcgttg
     9721 ttcactactt gcacggtcgg atcgtcctgc accagcgcca gatagctata catattgtcg
     9781 gcagatttac cgattggctt gctgacgaac tggcgtgtgg catcgtgatc ggctttgagt
     9841 gtttctacca gcttgctgtc ttccgccgcg agggattttt tattggcgat gtcgtaaatc
     9901 ggccgagctt ccgctttcgc ctgcgtcacc tgccatttac cgctgtcatt actgagttgt
     9961 aagtcgacca ccccaagatg atcgccccac atgcccggca ttaccgccgg aacaccattc
    10021 agcgtgcctt tggtgatatc agccccttcg atatcagcaa aatctttacc cgggaaaacg
    10081 gcgtgagcat gaccaaacat aatggcgtta acgcccggaa tttcactgag gtaataaact
    10141 gagttttccg ccattacttt atacggatcg gcagacagcc cggaatgcgc cagaacgaca
    10201 acgacatcgg cacctttctc gcgcatttca ggcacgtatt tgcgcacggt ttcggtaata
    10261 tcattcaccg tcacttttcc ggataaatta gctttatccc agcccatgat ttgcggcggc
    10321 acgacgccaa tatagccaat cttcagcgtc tgtttttttc cgtctttatc gaccacttcg
    10381 gtatctttaa ttaaatacgg tgtaaacatt ggctgtttgg ttctggcgtc aatgacgttg
    10441 gcatttacat aagggaattt cgctcccgcc agcgcatttt tcaggtaatc cagaccgtag
    10501 ttaaattcat gattgccgag tgtaccgacc gtataatcca gcgtattcag cgccttataa
    10561 accggatgaa catcacctgc ttttaatcct ttcgccgaca tgtaatcggc cagcggactc
    10621 ccctgaatca aatcgccgtt atcgaccagt acgctgtttt tcacttcatt gcgggcatcg
    10681 ttaatcaggc ttgccgtacg taccagtccg aatttttccg tggcggtgtc tttgtaataa
    10741 tcgaaatcca tcatgttgct atgcagatca gtggtttcca tgatacgcag atcgaccgtc
    10801 gctgcattca cactggcggc aatcagcgtg gccaggagcg ttgcgctaaa cttaatcatc
    10861 agggacatcc ttttatcatc gggaatacga aagaaaaggg agaataaacg tcttacttat
    10921 agaacagtga agaatgccac aattttacgc tttgaaaatg atgacactat cacagttggc
    10981 gcattcatta acgatagggt ataagtaaaa caataagtta acaccgctca cagagacgag
    11041 gtggagaaat gttagatcaa gtatgccagc ttgcacggaa tgcaggcgat gccattatgc
    11101 aggtctacga cgggacgaaa ccgatggacg tcgtcagcaa agcggacaat tctccggtaa
    11161 cggcagcgga tattgccgct cacaccgtta tcatggacgg tttacgtacg ctgacaccgg
    11221 atattccggt cctttctgaa gaagatcctc ccggttggga agtccgtcag cactggcagc
    11281 gttactggct ggtagacccg ctggatggta ctaaagagtt tattaaacgc aatggcgaat
    11341 tcaccgttaa cattgcgctc attgaccatg gcaaaccgat tttaggtgtg gtgtatgcgc
    11401 cggtaatgaa cgtaatgtac agcgcggcag aaggcaaggc ctggaaagaa gagtgcggtg
    11461 tgcgcaagca gattcaggtc cgcgatgcgc gcccgccgct ggtggttatc agccgttccc
    11521 atgcagatgc ggagctgaaa gagtatctgc aacagcttgg cgaacatcag accacgtcca
    11581 tcggctcttc gctgaaattc tgcctggtgg cggaaggaca ggcgcagctg tacccgcgct
    11641 tcggaccaac gaatatttgg gacaccgccg ctggacatgc tgtggccgca gctgccggag
    11701 cgcacgttca cgactggcag ggtaaaccgc tggattacac tccgcgcgag tcgttcctga
    11761 atcctgggtt cagagtgtct atttattaat taatttatgc agcaggtcca tcacttgctg
    11821 cacctcttcc tgagtaagcg ccccgtcttt ggcccattgc acgcgaccgt ctttatccag
    11881 caccactaca gcggaacttt tttcatccag ctgccaggca ccgcgtgcga cgccattgct
    11941 atcaacaata aactgcgacc agggataaag ctttttatta ctctccagac tgctgcgcac
    12001 aaacatgccg gaacccggaa ttgcgtcgtc ggtgttaaca atggtggtgg tctggtaacg
    12061 atcgtgcggt aacttcgctg atttaatcgc ttcaatcagc gtcgcgtttt tctcttttgc
    12121 agaggtgcga ccagcaatat gttgcagtac tcgcactttt cccactaact gcgcgctgtt
    12181 ccaggttttg tagctaaact gatctttatc aagcaccaac tcgccacgat cggtgatgcc
    12241 aatcggcggc actcgctgac cggtttcgaa ctgatgtgcg gaagccatca tcggcaacag
    12301 caggcaggtg agtgccagaa tcttgcgtag ggtcatggtg tttccttctt atgatatgca
    12361 ggtgatccga ccacttgggt ctgagtttta atcatatgtg ctatttatcg aatttcccgc
    12421 aagtgtgatg ccagtttgcg gtcaagcgca caaatcatat gaaaaatgaa tgcttatact
    12481 gaagaccgcg cttcggtaaa aagataattc tgaataattg taacctttag gtaaaaaaag
    12541 ttatacgcgg tggaaacatt gcccggatag tctatagtca ctaggcatta aaatttgcgc
    12601 ctcataatag ttgggccgat tgtggcaccg cacaggcgta atactcagca ggagataaca
    12661 atgaaaattt tccaacggta caacccactt caggtggcga agtacgtaaa gatcctgttc
    12721 cgtggacggt tatacatcaa ggacgttggc gcttttgaat ttgataaggg taagattctt
    12781 atcccaaaag tgaaggataa gctgcatttg tcagtgatgt ccgaagttaa ccgtcaggtt
    12841 atgcgtctgc aaacagagat ggcttaacca aagtgctatg cagtaataag acggctcctg
    12901 attcaggagc cgttgatgtt tctgggggtt acgcgacgct ttcttcttta tctttagcgt
    12961 caggcagttt tggcgaaagg gcggtggcct tgctgtcgat ccgggtcacc agcagctggt
    13021 cgatgcggta gttatcgata tccaccactt caaatttgta gccggcgaat ttcaccgaat
    13081 cggtgcgttt cgggatctta cgcagcataa acatcataaa gcctccgatg gtttcgtagt
    13141 tgcccgactg cgggaactcg tcaatatcca gcacgcgcat gacgtcgtca attggggtgc
    13201 cgccgtcaat cagccatgag ttctcatcac gggcgacaat ctgctcttcc agcccctgac
    13261 cgaccagatc gcccatcagc gtggtcatca cgtcgttgag ggtgatgatc cccaccacca
    13321 gcgcgtactc gttcatgatc accgcgaagt cttcacctgc ggttttaaaa ctttccaacg
    13381 cctctgaaag ggttaacgta tccggcacaa tcagcgtgtt gcgaatttgt acgccgctgt
    13441 tcagtgccag gctttggtta gccagcacgc ggttcagcag gtctttagaa tcgacataac
    13501 cgatgatgtg gtcaatatct tcattacaga cgaggaactt agagtgcgga tgttccgcca
    13561 ccttattctt caggctttgc tcatcttcgt ggagatcaaa ccaaatcacg ttttcacgcg
    13621 gtgtcattga agacggaacg gtacgggatt ccagctcaaa gacgttttca atcagctcgt
    13681 gttcctgttt acgtaacacg cccgccagcg caccggcttc cactaccgcg tagatgtcat
    13741 cagaagtgat gtcatcttta cgtaccattg gcagtttgaa aatacggaag atcatgttcg
    13801 ccaggccgtt gaagaaccac accagcgggg tgcaaacgta caggcagaag cgcatcgggt
    13861 tgatgatacg caaagccacc gcttctggcg caatcatacc gatgcgtttc ggggttaaat
    13921 ccgcaaacag gataaacatg ccagtcacta acgagaaaga gagaataaag ctcagttgct
    13981 cagagagttc tgccgacata tagcgggaga acaggctgtg aaaagctgga gaaaatgccg
    14041 catcaccgac gataccgccg agaatcgcaa ctgcgttcag accgatttgg accaccgtaa
    14101 agaacatgcc gggattttcc tgcatattca gaacgcgttg ggcatttata ttgccttcat
    14161 cagccagcag tttaagtttg attttgcgtg aggcggcaag tgagatctca gacatcgaga
    14221 agaacgcact tacagcgatc aagcagagta tgactaaaat actgtttaac atatcttatc
    14281 cgacctttca ggccggatcc tcggaagggg aagttgatta atttgtgtga aatacacatt
    14341 gaaagccgat tcgaagaaag tgaaccggca ttttcagtgg ctagtatagc gtaaggtact
    14401 gtaaagtcgc cagagggtta aaattcagac tgctgataaa tgcaatactg cctgatgcgc
    14461 tatgccgatc aggcctacgc agtccctgca atatattgaa tttggacgat tttgtaggcc
    14521 tggtaaggca ttcacgccgc attcggcata aacaaaacgc actttatcaa caatctacat
    14581 ttgtacccgt aacgctatgc ttccggtggc agacagacgc caattccgcc aattccacag
    14641 taaccatacg ggtttttatg cagatattgc tggtggtcat cttcggcata ataaaacggt
    14701 gtggcgttag cgatttccgt ggtgatgtga cgatcgtcat cggcggcaag catcgccgcc
    14761 tgaaaacgtt ccagactggc gcgagctgcg gcatcctgtt ctggggtcag cggataaatc
    14821 gctgaacgat actgcgtgcc gtggtcattg ccctgacgca ttccctgggc gggatcgtga
    14881 ttctcccaaa atacctgtag caactgctca tagctgatga cagaaggatc gtaaacaatg
    14941 cgtaccgctt cggcatgacc cgtatcaccg gagcacactt cccgataagt cggatttggc
    15001 gtatagccgc cggtatagcc tgcggcggtg ctgtaaacgc cgggtaactg ccagaacaga
    15061 cgctccacac cccagaaaca acccatcgca aaaatggcaa tctccattcc gtcaggtaca
    15121 ttggtcattg agtgaccgtt gaccgcatgc agcgtggcta cgggcatcgg ggtgttacgt
    15181 ccaggcaggg catcggcggg ggaaaccaga tgctttttat caaataaact catggtgtcg
    15241 ctctcccgaa aatcggtcat tggggttaag gttgtaacaa gaggcgtatt tgcacacaat
    15301 aaccaccgtg aataggtcta aagtaaaaca taagaaatat ttggggttta gtctgctttt
    15361 taatccatat tactggattt ttgttaagcc gtttaacggc gttccagggg caggaaaaaa
    15421 ggatattcag gagaaaatgt gcgctatatc cgacagttat gctgtgtaag cttactctgc
    15481 ttaagcggat ctgccgtcgc cgcgaacgtc cgtctacagg tcgaggggtt atcgggacag
    15541 ctggaaaaga acgttcgtgc gcagctttct acgattgaaa gtgatgaagt gacgccagac
    15601 cgtcgctttc gcgcacgcgt cgatgatgcc atccgcgaag gtctgaaagc gctgggttat
    15661 taccagccga ccattgaatt tgatctccgt ccaccgccaa agaaagggcg gcaggtattg
    15721 atcgccaaag tcacgccagg cgtgccggtg ttaattggcg gcaccgatgt ggtattgcgc
    15781 ggcggcgcgc ggaccgataa agactatttg aaattgctcg atactcgccc ggctattggc
    15841 acggtactga accagggcga ttatgaaaat ttcaaaaagt ccttaaccag cattgcgttg
    15901 cgtaaaggtt atttcgatag cgaatttacc aaagcgcagc tgggcattgc gctcggcctg
    15961 cataaagcct tctgggatat tgattataac agtggcgaac gttaccgctt tgggcatgtg
    16021 acctttgaag gatcacaaat ccgcgatgaa tacctgcaaa atctggtgcc gtttaaagag
    16081 ggcgatgagt acgaatcgaa agatctggcg gaacttaacc gtcgactttc tgctaccggc
    16141 tggtttaact cggtcgtagt ggctccacaa tttgataaag cgcgcgaaac gaaagtatta
    16201 cccttgacgg gcgtggtttc gccgcgaact gaaaacacca tcgaaaccgg ggtcggttac
    16261 tctacggacg tgggaccgcg cgtgaaagct acgtggaaaa agccgtggat gaactcttat
    16321 ggtcacagtc tgaccaccag taccagtatt tccgcgccgg aacagaccct cgacttcagc
    16381 tataaaatgc cgctgctgaa gaatccactg gaacaatatt atttggtgca gggcggtttt
    16441 aagcgcactg acctgaacga taccgaatct gactccacta cgctggtggc ttctcgctac
    16501 tgggatctct ccagcggctg gcagcgtgcc attaacctgc gctggagtct cgaccacttt
    16561 actcagggtg aaattaccaa caccacgatg ctgttttatc ctggggtgat gattagccgc
    16621 acgcgttctc gtggtggcct gatgccaacc tggggcgact cgcaacgcta ctctatcgac
    16681 tactccaaca cggcctgggg ctcagatgtc gatttctccg ttttccaggc gcagaacgtc
    16741 tggatccgca cactgtacga tcgccatcgt ttcgttacac gcggcacgct gggctggatt
    16801 gaaaccggtg atttcgacaa agtaccgccg gatctgcgtt ttttcgccgg gggcgatcgc
    16861 agtattcgcg gctacaaata caaatctatc gccccgaaat acgccaacgg tgacctgaaa
    16921 ggggcctcga agttgataac cggatcgctg gaataccagt acaacgtgac cggaaaatgg
    16981 tggggcgcgg tgtttgtcga tagtggcgaa gcggtaagcg atattcgccg cagcgacttt
    17041 aaaaccggta ccggggtcgg cgtgcgctgg gaatcgccgg tcgggccaat caaactcgat
    17101 tttgccgtac cggtcgcgga taaagacgaa cacgggttac agttttacat cggtctgggg
    17161 ccagaattat gagtttatgg aaaaaaatca gcctcggcgt ggttatcgtt atcttactgt
    17221 tgctgggatc ggtggcgttt ctggtgggca ccaccagcgg cctgcatctt gtatttaaag
    17281 cggcggatcg ctgggtgcca ggactggata ttggcaaggt caccggcggc tggcgcgatc
    17341 tcaccttgtc tgacgttcgt tatgagcagc caggcgtggc ggtaaaagcg ggtaatctgc
    17401 atctggcggt cggtcttgag tgcctgtgga acagtagcgt ttgtattaat gacctggcgc
    17461 tgaaagacat tcaggtcaac atcgacagta aaaaaatgcc tccttctgaa caggttgaag
    17521 aagaggaaga tagcggtccg ctggatctct ccacgccgta tcccatcacc ctgacacggg
    17581 tggcgctgga caacgtcaac atcaagattg atgacaccac ggtgtcggtg atggacttca
    17641 cctccggcct gaactggcag gagaaaaccc tgaccctgaa accgacgtcg ctgaaaggcc
    17701 tgctgattgc tctgccgaaa gtggcggaag tggcgcagga agaagtggtc gaaccgaaaa
    17761 ttgaaaatcc gcagccggag gaaaagccgc tcggcgaaac gctgaaagat ctcttttctc
    17821 gcccggtatt gccggaaatg accgacttgc atttgccgct taacctgaac attgaagagt
    17881 ttaaaggcga gcagttacgc gtgacgggcg acacggacat caccgtgcgc accatgctgc
    17941 tgaaagtgag cagcattgac ggcaatacta aactggacgc cctggatatc gattccagtc
    18001 aagggatcgt caacgccagc ggcacggcgc agctgtcaga caactggccg gtggatatca
    18061 ccctcaacag cacactgaac gtggagccgt tgaaaggtga aaaagtgaag ctaaaagtgg
    18121 gcggcgcgct gcgcgaacag ctggagattg gcgttaatct ttccggtccg gtggatatgg
    18181 atttacgcgc ccagacgcga ctggcggaag ccggattgcc actcaacgtg gaagtgaaca
    18241 gcaaacagct ttactggccg ttcaccggtg agaagcagta tcaggcggat gatctgaaac
    18301 tgaaacttac cggcaaaatg accgattaca cgctctctat gcgtacggca gtgaagggac
    18361 tggagatccc gccagccacc attacccttg atgccaaagg taatgaacag caggtcaatc
    18421 tcgacaaact caccgtcgcg gcgctggaag ggaaaactga actcaaggcg ttgctcgact
    18481 ggcagcaggc cattagttgg cgcggtgagc taacgcttaa cggcattaac accgccaaag
    18541 agatcccaga gtggccgtcg aaactcaatg gcttgattaa aacccgcggt agcctgtacg
    18601 gcggcacctg gcagatggag gtgccagaac tgaagctgac cggtaacgtc aaacagaaca
    18661 aagtgaacgt tgacggcacg ctgaaaggca acagttatat gcagtggatg atcccagggc
    18721 ttcatctgga actggggcca aacagtgccg aagtgaaagg cgagctgggg gtaaaagatc
    18781 tcaatcttga tgccaccatc aacgcgccgg ggctggataa cgcgctgccg gggcttggcg
    18841 gtacagcgaa agggctggtg aaagttcgcg gcacggtgga agcgccacaa ctactggcgg
    18901 acatcaccgc gcgcggcctg cgctggcagg agctaaccgt ggcgcaggtt cgcgtggaag
    18961 gcgatatcaa atccaccgtt cagattgcgg gtaaactcga cgtgcgcgtt gagcaaattt
    19021 cacagccgga tgtaaatatc aacctcgtca ccctgaatgc caaaggcagc gaaaaacagc
    19081 acgagttaca gttgcggatt cagggcgagc cggtttccgg gcagcttaat ctggcaggaa
    19141 gttttgatcg caaagaagaa cgctggaagg gaactcttag caatacccgc ttccagacgc
    19201 cggtcggccc gtggtcgctg acccgcgata ttgcgctgga ttaccgcaat aaggagcaaa
    19261 aaatcagcat cgggccacac tgctggctta acccgaatgc ggaactgtgc gtgccgcaaa
    19321 ctatcgatgc gggggccgaa gggcgtgcgg tggtgaatct caaccgcttc gacctcgcca
    19381 tgctgaaacc gtttatgcca gaaaccactc aggccagcgg tatcttcacg ggtaaagcgg
    19441 atgttgcctg ggacaccacg aaagaggggc tgccgcaggg cagtatcacc ctttcggggc
    19501 gtaacgtgca ggtaacgcaa accgtcaacg atgcggcgct gcccgtggcg tttcagacac
    19561 tgaatctgac ggcggaattg cgtaacaacc gtgccgaatt gggctggacc atccgcctga
    19621 ccaataacgg ccagtttgat ggacaggtgc aggtgaccga tccgcaaggc cgccgtaatc
    19681 ttggtggcaa cgtcaatatc cgtaacttca accttgcgat gataaacccc atctttaacc
    19741 gtggggaaaa agcagcgggg atggtgagtg ccaacttgcg tctgggtggt gatgtgcaaa
    19801 gcccgcagtt gtttggtcag cttcaggtta cgggtgtgga tatcgacggc aactttatgc
    19861 cgtttgatat gcaaccgagc cagcttgcgg tcaactttaa tggtatgcgc tcgacgcttg
    19921 ccggtacagt acggacccag cagggtgaaa tctacctgaa cggtgatgcc gactggagcc
    19981 aaattgaaaa ctggcgggcg cgggtaacgg cgaaaggcag taaagtgcgg atcaccgtgc
    20041 cgccgatggt acgaatggat gtatcgccag atgttgtctt cgaggctaca ccaaacctgt
    20101 ttaccctcga tggtcgcgtg gatgtcccgt gggcgcgcat cgtggtgcac gatctgccgg
    20161 aaagcgcagt aggcgtctcc agcgatgtgg tgatgcttaa cgataacctg caacctgaag
    20221 agccgaaaac ggcgtcgatt ccgattaaca gtaacctgat tgtccacgtt ggcaacaatg
    20281 tgcgcattga cgcctttggc ctgaaagcgc ggctgacggg cgatcttaac gtcgttcagg
    20341 acaaacaagg gctgggcctg aacgggcaga tcaacatccc tgaagggcgc ttccatgcct
    20401 atggtcagga tctgattgtg cgtaaaggtg agttactgtt ctctggtccg ccggatcaac
    20461 cgtatcttaa tattgaagct attcgtaacc cggatgctac agaagacgac gtaatcgccg
    20521 gagttcgcgt cactggtctg gcggacgaac cgaaagcgga gatcttctct gacccggcga
    20581 tgtcgcaaca agctgcattg tcttatttgc tacgtggaca agggctggag agcgatcaga
    20641 gcgacagtgc ggcaatgacc tcgatgctga ttggtctggg ggttgcgcaa agtggccaga
    20701 ttgtgggtaa aatcggcgag acgtttggcg taagcaattt agcgctcgac acccagggag
    20761 taggcgactc ctcccaggta gtggtcagcg gctatgtatt gccaggtctg caagtgaaat
    20821 atggcgtggg tatatttgac tctatagcaa cactcacgtt acgttatcgc ctgatgccta
    20881 agctatatct ggaagccgtg tctggtgtag accaggcact ggatttgctc tatcagttcg
    20941 agttttagca atgcgaatat ttgtctacgg cagtttacgc cacaaacaag gcaacagtca
    21001 ctggatgacc aatgcccagt tactgggcga tttcagtatc gataactacc agttgtatag
    21061 cctgggccac tatccaggcg cagttccggg gaacggaaca gtacacggtg aagtttatcg
    21121 tattgacaac gccacgctgg ccgaacttga tgccttgcgc accaggggcg gtgaatacgc
    21181 gcgccagttg attcagacgc cgtacgggag tgcatggatg tacgtttatc aacgacccgt
    21241 cgatggatta aagctaattg aaagcggcga ctggttagac agggataagt aaccatatgc
    21301 atacgccacc ttcgggtggc gttgtttttt gcgagacgac tcgcattctg ttttgtaatt
    21361 ccctcaccct ttgcttttct ctccgagccg ctttccatat ctgttaacgc ataaaaaact
    21421 ctgctggcgt tcacaaatgc gcaggggtaa aacgtttcct gtagcaccgt gagttatact
    21481 ttgtataact taaggaggtg cagatgcgta ttaccataaa aagatggggg aacagtgcag
    21541 gtatggtcat tcccaatatc gtaatgaaag aacttaactt acagccgggg cagagcgtgg
    21601 aagcgcaggt gagcaacaac caactgattc tgacacccat ctccaggcgc tactcgcttg
    21661 atgaactgct ggcacagtgt gacatgaacg ccgcggaact tagcgagcag gatgtctggg
    21721 gtaaatccac ccctgcgggt gacgaaatat ggtaaagaaa agtgaatttg aacggggaga
    21781 cattgtgctg gttggctttg atccagcaag cggccatgaa cagcaaggtg ctggtcgacc
    21841 tgcgcttgtg ctctccgttc aagcctttaa tcaactggga atgacgctgg tggcccccat
    21901 tacgcagggc ggaaattttg cccgttatgc cggatttagc gttcctttac attgcgaaga
    21961 aggcgatgtg cacggcgtgg tgctggtgaa tcaggtgcgg atgatggatc tacgcgcccg
    22021 gctggcaaag cgtattggtc tggctgcgga tgaggtggtg gaagaggcgt tattacgctt
    22081 gcaggcggtg gtggaataag gtgtgtttat ttatcgcggg cataaaaaaa cccttactaa
    22141 ccgaagctcg gcgttcaggg ttattgcgct agaagaactt atttattctt tgcgcgctcg
    22201 aaggaagcaa cgatttcagc tttagcggct tctgcgtttt cccaaccttc aactttcacc
    22261 cacttgcctt tttcgaggtc tttgtagtgc tcgaagaagt gagcgatctg cgctttcagc
    22321 agctctggca gatcgttaac gtctttaatg tgatcgtatt ctttgctcag cttgctgtgc
    22381 ggaaccgcaa ccagtttcgc atcttcaccg gcttcgtcgg tcattttcag aacgccaacc
    22441 ggacggcaac ggatcacaga acccggctgc agcgggtacg gagtcgggac cagtacgtca
    22501 accgggtcac cgtccagaga cagggtgtgg ttgatgtaac cgtagttgca cggatagaac
    22561 atcgcggtgg acatgaagcg gtcaacgaac agtgcgccgc tctctttgtc gatttcgtat
    22621 ttgatcggat ctgcgttagc cgggatctca ataacaacgt agatgtcttc cggcagatct
    22681 ttacccgcag ggacgttgag taagctcatg tctgtttcct ttaaaaatat gtggcaaaca
    22741 agtgccgagt attatagcca actcgcgccg aatgtctttg cttgttttcg tcttcatttt
    22801 cctttttcgc cagttttaag acggtataca gagcaggaaa atccataacc gtagccgcat
    22861 ttttcatagt gagatgaaag cgattacaaa cttgtgatta acgttttatt tacttttttg
    22921 aagtgtgatg taacgcaatc cgttacataa cgaattgtct atagtttttt cgcgaacatc
    22981 ttttcaccaa taataattac cccgacgagg acaaccctat gtggaaacgc ttacttgtag
    23041 tctctgcagt ctcggcagcc atgtcgtcta tggcgttggc cgctccatta accgtaggat
    23101 tttcgcaggt cggatcggaa tccggctggc gcgctgcaga aaccaatgtg gcgaaaagtg
    23161 aggccgaaaa acgcggaatt acgttgaaaa ttgccgatgg tcagcaaaag caggaaaacc
    23221 agattaaagc ggtacgttcc ttcgttgcac aaggggtgga tgcgatcttt atcgctccag
    23281 tggttgcgac gggttgggaa ccggtattaa aagaggcgaa agatgccgaa atcccggtct
    23341 tcttgcttga ccgttccatc gatgtgaaag acaaatctct ctatatgacc accgtcactg
    23401 ccgacaacat cctcgaaggc aagttgattg gtgactggct ggtaaaagaa gtgaatggca
    23461 aaccatgcaa cgtggtggaa ctgcagggca ccgttggggc cagcgtcgcc attgaccgta
    23521 agaaaggctt tgccgaagcc attaagaatg cgccaaatat caaaatcatc cgctcgcagt
    23581 caggtgactt cacccgcagt aaaggcaaag aagttatgga gagctttatc aaagcggaaa
    23641 acaacggcaa aaacatctgc atggtttacg cccataacga tgacatggtg attggtgcaa
    23701 ttcaggcaat taaagaagcg ggcctgaaac cgggcaaaga tatcctcacg ggttccattg
    23761 acggcgtacc ggatatctat aaagcgatga ttgatggcga agcgaacgcc agcgttgaac
    23821 tgacgccgaa catggcaggc cccgcttttg acgcgctgga gaaatacaaa aaagacggca
    23881 ccatgcctga aaagctgacc ctgaccaaat ccacccttta tctgcctgat accgcaaaag
    23941 aagagttaga gaagaagaaa aatatggggt attgagggtt gctatgcctg atgccgattc
    24001 gtaggccgga taaggcgctc gcgccgcatc cggcgatggt gcactgaagc ctgatgcgac
    24061 gcttaccgcg tcttatcatg cctactggga gcacgcttta caccggggga aaccatgacg
    24121 accgaccaac accaggagat cctccgcacc gaaggattaa gtaaattttt ccccggcgtc
    24181 aaagcgttag acaacgttga tttcagcctg cgccgtggcg aaatcatggc gctgcttggt
    24241 gaaaacgggg cgggaaaatc aacgctaatc aaagcattaa ctggtgtata ccacgccgat
    24301 cgcggcacca tctggctgga aggccaggct atctcaccga aaaataccgc ccatgcacaa
    24361 caactcggta tcggcaccgt ctatcaggaa gtcaacctgc tacccaatat gtcggttgct
    24421 gataatctat ttataggccg cgaacccaaa cgtttcggcc ttctacgccg taaagagatg
    24481 gaaaagcgcg ccaccgaact gatggcatct tacggtttct ccctcgacgt gcgcgaaccg
    24541 cttaaccgtt tttcggtggc gatgcagcaa atcgtcgcca tttgccgggc tatcgatctc
    24601 tccgccaaag tgctgatcct cgatgaaccc accgccagcc tcgacaccca ggaagtggag
    24661 ctattatttg gcctgatgcg tcagttgcgc gatcgcggcg tcagcctgat ctttgtcact
    24721 cactttctcg atcaggtcta tcaggtcagc gatcggatca ccgtcttacg caacggcagt
    24781 ttcgtaggct gtcgggaaac gcgcgagcta ccgcagatcg aactggtaaa aatgatgctg
    24841 gggcgcgagc tggataccca cgcgctacag cgtgccgggc gaacattgtt gagcgacaaa
    24901 cccgttgccg cgttcaaaaa ttacggcaaa aaaggaacga tagcaccgtt tgatctcgaa
    24961 gtgcgccccg gcgagatcgt cggtctggct ggcttgctgg gatccggacg taccgaaacc
    25021 gccgaagtga tcttcggtat caaacctgct gacagcggca cggcgttgat caaaggcaaa
    25081 ccgcaaaccc tgcgatcgcc acatcaggct tcagtactgg gcatcggatt ctgcccggaa
    25141 gacaggaaaa ccgatggcat catcgctgcc gcctcggtgc gggaaaatat catcctcgct
    25201 ctccaggccc agcgcggctg gctacgaccc atttcccgca aagagcagca agagattgcc
    25261 gaacgcttta tccgccagct tggcattcgc acaccttcaa ctgaacaacc gattgaattt
    25321 ctctctggcg gcaatcagca aaaagtgttg ctttcacgtt ggctactgac acgaccgcaa
    25381 tttctgatcc tcgacgagcc aacccgcggc attgatgttg gtgcccacgc cgagatcatc
    25441 cgcctgattg aaacgctgtg tgctgacggt ctggcgctgt tggtgatctc ctccgaactg
    25501 gaagagctgg tgggctatgc cgatcgggtg attatcatgc gcgatcgcaa acaggtggcg
    25561 gagatcccgc tggcagagct ttccgttccg gcgatcatga acgccattgc ggcgtaagga
    25621 gaacagtgtg atgcctcaat ctctcccgga cactacgccg ccgaaaaggc gctttcgctg
    25681 gccaacggga atgccgcagc tggcggcgct attgctggtg ctgctggtcg atagcctggt
    25741 agccccgcat ttctggcagg tggtgcttca ggatgggcgt ttgttcggta gccctatcga
    25801 cattcttaac cgtgcagctc ccgttgcgtt actggcgatt ggtatgacgc tggtgatcgc
    25861 caccggtggg attgatctct ccgtaggggc ggtgatggct atcgccggag ccacaacggc
    25921 tgcgatgacg gtcgcgggat tcagcctgcc gattgttttg ttaagcgccc tgggcaccgg
    25981 catcctggcg ggattgtgga acggcatact ggtagcgatc ctcaaaattc agccgtttgt
    26041 cgccaccctg atcctgatgg tcgccgggcg cggcgtggcg caactgatca ccgccgggca
    26101 gatcgtcacg tttaactcgc cggatctctc atggtttggc agcggatcgc tgttgttcct
    26161 gccaacgccg gtcattatcg cggtgctgac gcttctcctg ttctggctgt tgacccgcaa
    26221 aacggcgctg gggatgttta tcgaagccgt tggtatcaac attcgggcgg caaaaaatgc
    26281 cggggtaaac acgcggatca tcgtcatgct cacctacgtg ttgagcgggc tgtgtgcggc
    26341 gattgcgggc attatcgtgg cggcggatat tcgcggtgcc gatgccaata acgccgggtt
    26401 atggctggag ctggacgcca ttcttgcggt ggtgattggc ggcggatcgc tgatgggcgg
    26461 gcgctttaac ctgctgctct cagtcgtggg ggcgctgatt attcagggga tgaacaccgg
    26521 aattttactt tctggctttc cgccggagat gaaccaggtc gtaaaagcgg tggtggtgct
    26581 ttgtgtgctg attgttcagt cgcaacgctt tatcagtctg attaaaggag tacgtagcca
    26641 tgataaaacg taacttgccg ctgatgatca ccatcggcgt ctttgtgctg ggctatctct
    26701 actgcctgac ccagtttccc ggttttgctt ccacaagagt gatctgcaat atcctgaccg
    26761 ataacgcctt tcttgggata atcgccgttg gcatgacctt tgtgatcctc tctggcggta
    26821 tcgatctctc cgtcggttcg gtgatcgcct ttactggcgt gtttctggca aaagtgattg
    26881 gcgatttcgg cctctcgccg ctgctggcgt ttccgctagt gctggtgatg ggctgtgcct
    26941 ttggcgcatt tatggggctt ttgatcgacg ccctgaagat cccggcattt atcattaccc
    27001 tggcaggaat gttctttttg cgcggcgtca gctatctcgt ttcagaagag tcgatcccga
    27061 taaaccatcc catttatgac acgctctcaa gccttgcgtg gaaaatccct ggcggcggtc
    27121 gcttaagtgc gatgggactg ctgatgctgg cggtggtggt tatcggcata ttcctcgcgc
    27181 atcgtacccg ttttggtaat caggtatacg ccattggcgg caacgcaacg tcggcaaacc
    27241 tgatggggat ttctacccgc agcaccacta ttcgcattta tatgctctcc accggactgg
    27301 caacgctggc ggggattgtc ttctcgattt atacccaggc cggatatgcg ctggcgggcg
    27361 taggtgtgga actggatgct atcgcctcag tggtaattgg cggtacgctt ttgagcggtg
    27421 gcgttggaac ggtattaggg acgctttttg gcgtggcgat tcaggggcta attcagactt
    27481 atatcaactt tgacggtacg ctgagttcct ggtggacgaa aatcgccatc ggcattttgc
    27541 tgttcatttt tattgcctta cagcgtggat taacggtgct gtgggagaat cgtcagagtt
    27601 cgccagtgac aagagtcaat attgcgcagc gataaaaacg cctctccgtg tggagaggcg
    27661 caggagatta cgcgtccggg aattcacgga taaagcgttc gacatcttca accatatggt
    27721 cgttgccgac aaagaacgaa cggcgctggt gcagagtttc cgggatgata tccagaatac
    27781 gctctttgcc atcgctcgct ttaccgcccg cttgttccgc caggaatgcc atcgggttgc
    27841 actcatacag caaacgcagt ttgccgtccg ggtggctggc ggtgcttggg tagagataaa
    27901 taccgccttt cagcaggtta cggtggaaat ccgcgaccag tgaaccgata taacgtgagg
    27961 tatacgggcg gttggtggat ttatcttctt cctggcagaa tttaatgtac ttcttcaccc
    28021 cgttcggaaa cttaatgtag tttccttcgt tgatggagta ggttttgcct ttctccggga
    28081 agcgcatccg ttcctggcac aggcagaaaa cgcccagcga aggatcgtaa gtaaaggcgt
    28141 gaacaccgca tccggtggtg taaaccagca tggtagagga gccgtatacc acgtaacctg
    28201 ccgcaacctg tttgttacca ggctggagga aatcttcttc cgttaccggc gtgccaacag
    28261 gcgtaacgcg gcggtagatg gagaaaatgg taccgacaga gacgttaaca tcgatgttgg
    28321 acgagccatc cagggggtcc atcagcacca cgtattttgc gtgttcacag ccttcaaaga
    28381 cgacaatctc atcttcttct tcagaggcaa tgcccgcaac gatatcgcgt gctttcagtg
    28441 cggctttcag tttttcatta gcgaacaagt cgagtttctg ctgaacctcg ccctgcacgt
    28501 tctcagcacc gctggcaccc aggatatcaa ccagtcctgc tttgttgata tcgcgatgga
    28561 taatcttggc gcccagtttt attgccgaca gcaaagcagt gagctcaccg gtagcatgag
    28621 aaaactcgtg ctgcttttcg acaataaatt cacctaacgt tttcataaaa ctttccctgc
    28681 aatgtttatg gagtaaagcg accgcaacaa tcttaacaaa taatctcaat gttgcgctca
    28741 ggtgaatcgc gccagcaaat tgcggattat cctgaaatgc gtttctcact tgcccgacat
    28801 atgcgtaaaa tgagcggcag attaaaaaag gatagtgacg tatgcgcatt catattttag
    28861 gaatttgtgg cacatttatg ggcggtctgg cgatgctggc gcgccagtta ggccatgaag
    28921 taacgggttc ggacgccaat gtgtatccgc cgatgagcac cttacttgag aagcaaggca
    28981 ttgagctgat tcagggttac gatgccagcc agctcgatcc gcagccggat ctggtgatta
    29041 ttggcaacgc catgacccgt ggaaatccgt gtgtggaagc ggtactggaa aaaaacatcc
    29101 cttatatgtc aggtccacag tggctgcacg attttgtgct gcgcgaccgc tgggtgctgg
    29161 ccgttgccgg tacacacggc aaaactacca ccgcgggaat ggcgacctgg attctggaac
    29221 agtgcggtta caaaccggga tttgtgatcg gcggtgtgcc ggggaacttt gaggtttcgg
    29281 cgcgtctggg cgaaagcgac ttctttgtta tcgaagcgga tgagtatgac tgcgccttct
    29341 tcgacaaacg ctctaaattt gtccattact gcccgcgtac gctgatcctc aacaaccttg
    29401 agttcgatca cgccgatatc tttgacgacc tgaaagcgat ccagaaacag ttccaccatc
    29461 tggtgcgtat cgttccgggg cagggccgta ttatctggcc ggaaaatgac atcaacctga
    29521 aacagaccat ggcgatgggc tgctggagcg agcaggagct ggtgggtgag cagggtcact
    29581 ggcaggcgaa aaagctgacc actgacgctt ctgaatggga agtcttgctg gatggcgaaa
    29641 aagtgggcga agtgaaatgg tcgctggtgg gcgaacataa tatgcacaat ggcctgatgg
    29701 cgattgcggc ggctcgccat gttggtgtag cgcctgcaga tgccgctaac gcgctgggtt
    29761 cgtttattaa cgctcgtcgc cgtctggagt tgcgtggtga agcgaatggc gtgacggtat
    29821 atgacgattt tgcccatcac ccgacggcaa ttctggcaac gcttgcggcg ctgcgtggca
    29881 aagttggtgg tacggcgcgc attattgctg tgctggagcc gcgctcgaat accatgaaaa
    29941 tggggatctg caaagacgat ctggcacctt cattaggtcg tgccgatgaa gtcttcctgc
    30001 tgcaaccggc gcatattccg tggcaggtgg cagaagtggc agaagcctgc gttcagcctg
    30061 cacactggag tggcgatgtg gatacgctgg cagatatggt ggtgaaaacc gctcagcctg
    30121 gcgaccatat tctggtgatg agcaacggcg gttttggtgg gatccatcag aaactgctgg
    30181 atggtctggc gaagaaggcg gaagctgcgc agtaattcgg cctcagcctg atatagtgtg
    30241 cgtaggccgg ataaggcgtt cacgccgcat ccggcatttg agcatagtgc ctgatgcgac
    30301 gcttgatgcg tcttatcagg cctacaggtg caccgcatcc ggcaaaccat actcacatca
    30361 acaacgaaaa ttacccttca ttctccgcca gttcgcgcag atactggaaa atctggcgtg
    30421 cggatttcgg cggcttattc ccttctttct ctttcttcgc gttgcggatc agagtacgca
    30481 gctgctgacg atcggcatcg tgccacagat ttaatacctc agcgatggcg tcatcacctt
    30541 gatcgatcag acgatcgcgc aggttttcca gtttatggaa cagcaccacc tgctggttgt
    30601 gacggttttt cagcttatcc agcgcctgac ggatcggttc tacatcgcgc tggcgcagca
    30661 tcttaccgat gagctgcaac tggcggcggc gaccttccat cttaatacgc tgggcgagct
    30721 caatagccgc gcgcagatcc gcatctaacg ggatcttatc cagcgcgttt ttccccagat
    30781 caacaatttc cgcgccaagg cgttttagct cctcggcatc acgtttaatt tcacttttac
    30841 tgacccagat aatttcatcg tcttcgtctt cgatgtcatc accgggaacg tcgtcgagcc
    30901 agtcttcggg ctgcttagtc atctcaggct ccttaaaaaa agaggctaat gttaccagtt
    30961 aagatgcgca ctgaaaaacg gttctctgtt agacttcaga gaaactctct acattatggc
    31021 acttgcaatg aaagtaatct ctcaagttga agcgcagcgc aagattctgg aagaagcagt
    31081 ttccactgcg ctggagttgg cctcaggcaa atcggacggt gcggaagttg ccgtcagcaa
    31141 gaccaccggc attagcgtaa gcacgcgtta tggtgaagtg gagaatgttg aattcaatag
    31201 cgatggcgcg ctggggatca ctgtttatca ccagaaccgc aaaggtagcg catcatccac
    31261 cgatttaagc ccgcaggcca ttgcccgtac tgtacaggcg gcgctggata ttgcccgtta
    31321 tacctcgcca gatccctgtg ccggcgtggc agacaaagag ctgctggcct ttgacgcacc
    31381 agatctcgac ttgttccacc ctgcggaagt ttccccggat gaagccattg aactggcggc
    31441 ccgcgcagaa caggcggcat tgcaggcgga caaacgcatt accaataccg aaggtggcag
    31501 ctttaacagc cactacggtg tcaaagtttt tggcaacagc cacggcatgt tgcagggtta
    31561 ctgctcaacg cgtcattcgc tctccagctg tgtaattgcc gaagaaaatg gcgatatgga
    31621 gcgtgattac gcctacacca ttggccgcgc gatgagcgat ctgcaaacgc cagagtgggt
    31681 cggggccgac tgtgctcgcc gtactttatc gcgcctgtca ccgcgtaaac tctccaccat
    31741 gaaagcgcca gtcatttttg ccaatgaagt ggcaaccggg ctttttggcc atctggtggg
    31801 ggcgatagcg ggtggctcgg tttaccgtaa atctaccttt ctgctggatt cgctgggtaa
    31861 acaaattctg ccggactggc tgaccattga agagcatccg catctgctga aagggctggc
    31921 gtcgacgcca ttcgacagcg aaggtgtgcg caccgagcgt cgcgatatta ttaaagatgg
    31981 catcctgact cagtggctgc tgaccagcta ctcggcgcgg aaactggggc tgaaaagcac
    32041 cggacatgcg ggcggtattc acaactggcg gattgccgga caagggctaa gcttcgaaca
    32101 aatgctcaaa gagatgggca ccgggctggt ggtgacggag ctgatgggcc agggcgtgag
    32161 tgccattacc ggtgattatt cccgtggtgc agcgggcttc tgggtagaga atggcgaaat
    32221 tcagtatccc gttagtgaaa tcaccatcgc aggtaattta aaagatatgt ggcgcaatat
    32281 tgtcaccgtc ggtaacgata ttgaaacacg cagtaatata cagtgtggtt ctgtgctgtt
    32341 gccggagatg aaaatcgccg ggcagtaaaa aactggcgcg acctgccgcg ctataaacta
    32401 aaaaattaca caaaatcatt cgcactgcat cgagacggaa agtgaatgag ggccggggag
    32461 cgtactcgca gtacgtgacc cgggcgaatg agcgtagccg tcgatgaggc agtgtgaagg
    32521 atgaagtgta aataaaaaag gaagtgagca atgcgtaaaa gcctgttagc tattcttgca
    32581 gtctcctcgt tggtattcag ttctgcgtcg tttgccgctg atcttgaaga
//
LOCUS       DAZGJW010000044        32514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZGJW010000044 DAZGJW010000000
VERSION     DAZGJW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.044"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            10..612
                     /gene="yebB"
                     /locus_tag="ACHKIK_002043"
     CDS             10..612
                     /gene="yebB"
                     /locus_tag="ACHKIK_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO9083215.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(614..1135)
                     /gene="ruvC"
                     /locus_tag="ACHKIK_002044"
     CDS             complement(614..1135)
                     /gene="ruvC"
                     /locus_tag="ACHKIK_002044"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO9083216.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1170..1910)
                     /gene="yebC"
                     /locus_tag="ACHKIK_002045"
     CDS             complement(1170..1910)
                     /gene="yebC"
                     /locus_tag="ACHKIK_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9083217.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1939..2391)
                     /gene="nudB"
                     /locus_tag="ACHKIK_002046"
     CDS             complement(1939..2391)
                     /gene="nudB"
                     /locus_tag="ACHKIK_002046"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO9083218.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2509..4281)
                     /gene="aspS"
                     /locus_tag="ACHKIK_002047"
     CDS             complement(2509..4281)
                     /gene="aspS"
                     /locus_tag="ACHKIK_002047"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO9083219.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4591..5157
                     /gene="yecD"
                     /locus_tag="ACHKIK_002048"
     CDS             4591..5157
                     /gene="yecD"
                     /locus_tag="ACHKIK_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO9083220.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5154..5972
                     /gene="yecE"
                     /locus_tag="ACHKIK_002049"
     CDS             5154..5972
                     /gene="yecE"
                     /locus_tag="ACHKIK_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO9083221.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAACPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6025..6420
                     /gene="yecN"
                     /locus_tag="ACHKIK_002050"
     CDS             6025..6420
                     /gene="yecN"
                     /locus_tag="ACHKIK_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO9083222.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6461..7204
                     /gene="cmoA"
                     /locus_tag="ACHKIK_002051"
     CDS             6461..7204
                     /gene="cmoA"
                     /locus_tag="ACHKIK_002051"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO9083223.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7201..8172
                     /gene="cmoB"
                     /locus_tag="ACHKIK_002052"
     CDS             7201..8172
                     /gene="cmoB"
                     /locus_tag="ACHKIK_002052"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO9083224.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8337..10766)
                     /gene="torZ"
                     /locus_tag="ACHKIK_002053"
     CDS             complement(8337..10766)
                     /gene="torZ"
                     /locus_tag="ACHKIK_002053"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO9083225.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIRVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(10791..11891)
                     /gene="torY"
                     /locus_tag="ACHKIK_002054"
     CDS             complement(10791..11891)
                     /gene="torY"
                     /locus_tag="ACHKIK_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO9083226.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(12279..13025)
                     /gene="cutC"
                     /locus_tag="ACHKIK_002055"
     CDS             complement(12279..13025)
                     /gene="cutC"
                     /locus_tag="ACHKIK_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO9083227.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(13039..13605)
                     /gene="yecM"
                     /locus_tag="ACHKIK_002056"
     CDS             complement(13039..13605)
                     /gene="yecM"
                     /locus_tag="ACHKIK_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO9083228.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            13821..15554
                     /gene="argS"
                     /locus_tag="ACHKIK_002057"
     CDS             13821..15554
                     /gene="argS"
                     /locus_tag="ACHKIK_002057"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO9083229.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYESLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(15596..15640)
                     /gene="yecV"
                     /locus_tag="ACHKIK_002058"
     CDS             complement(15596..15640)
                     /gene="yecV"
                     /locus_tag="ACHKIK_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFO9083230.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            15731..16219
                     /gene="yecT"
                     /locus_tag="ACHKIK_002059"
     CDS             15731..16219
                     /gene="yecT"
                     /locus_tag="ACHKIK_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO9083231.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(16339..16731)
                     /gene="flhe"
                     /locus_tag="ACHKIK_002060"
     CDS             complement(16339..16731)
                     /gene="flhe"
                     /locus_tag="ACHKIK_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO9083232.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(16731..18809)
                     /gene="flhA"
                     /locus_tag="ACHKIK_002061"
     CDS             complement(16731..18809)
                     /gene="flhA"
                     /locus_tag="ACHKIK_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO9083233.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PVEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMELQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHAPRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(18802..19950)
                     /gene="flhB"
                     /locus_tag="ACHKIK_002062"
     CDS             complement(18802..19950)
                     /gene="flhB"
                     /locus_tag="ACHKIK_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO9083234.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(20152..20796)
                     /gene="cheZ"
                     /locus_tag="ACHKIK_002063"
     CDS             complement(20152..20796)
                     /gene="cheZ"
                     /locus_tag="ACHKIK_002063"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO9083235.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(20807..21196)
                     /gene="cheY"
                     /locus_tag="ACHKIK_002064"
     CDS             complement(20807..21196)
                     /gene="cheY"
                     /locus_tag="ACHKIK_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO9083236.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(21211..22260)
                     /gene="cheB"
                     /locus_tag="ACHKIK_002065"
     CDS             complement(21211..22260)
                     /gene="cheB"
                     /locus_tag="ACHKIK_002065"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO9083237.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(22263..23123)
                     /gene="cheR"
                     /locus_tag="ACHKIK_002066"
     CDS             complement(22263..23123)
                     /gene="cheR"
                     /locus_tag="ACHKIK_002066"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO9083238.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRCFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(23142..24746)
                     /gene="tap"
                     /locus_tag="ACHKIK_002067"
     CDS             complement(23142..24746)
                     /gene="tap"
                     /locus_tag="ACHKIK_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO9083239.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNNDLEHQATWLESNQLSDFLTAPVQESQNAF
                     DVNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQP
                     LAIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHI
                     GIAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTA
                     QAGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRG
                     FAVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVD
                     DIMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSR
                     VAVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(24792..26453)
                     /gene="tar"
                     /locus_tag="ACHKIK_002068"
     CDS             complement(24792..26453)
                     /gene="tar"
                     /locus_tag="ACHKIK_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO9083240.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQAPNWETF"
     gene            complement(26598..27101)
                     /gene="cheW"
                     /locus_tag="ACHKIK_002069"
     CDS             complement(26598..27101)
                     /gene="cheW"
                     /locus_tag="ACHKIK_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO9083241.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(27122..29086)
                     /gene="cheA"
                     /locus_tag="ACHKIK_002070"
     CDS             complement(27122..29086)
                     /gene="cheA"
                     /locus_tag="ACHKIK_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO9083242.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(29091..30017)
                     /gene="motB"
                     /locus_tag="ACHKIK_002071"
     CDS             complement(29091..30017)
                     /gene="motB"
                     /locus_tag="ACHKIK_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO9083243.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(30014..30901)
                     /gene="motA"
                     /locus_tag="ACHKIK_002072"
     CDS             complement(30014..30901)
                     /gene="motA"
                     /locus_tag="ACHKIK_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO9083244.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQMTTEEA"
     gene            complement(31028..31606)
                     /gene="flhC"
                     /locus_tag="ACHKIK_002073"
     CDS             complement(31028..31606)
                     /gene="flhC"
                     /locus_tag="ACHKIK_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO9083245.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(31609..31959)
                     /gene="flhD"
                     /locus_tag="ACHKIK_002074"
     CDS             complement(31609..31959)
                     /gene="flhD"
                     /locus_tag="ACHKIK_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO9083246.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         7707 a         8815 c         7877 g         8115 t
ORIGIN      
        1 aatgacaacg tgaatattaa ttaccctgct gaatatgaaa ttggtgatat cgtctttaca
       61 tgtataggtg ctgccttatt tggtcaaata tcagctgcat caaactgctg gagtaatcac
      121 gtcgggatca ttatcggtca taacggtgaa gactttctgg ttgcagaaag ccgtgttccc
      181 ctctcaacca tcactacgct atcccgtttt attaaacgct ctgctaatca acgctatgct
      241 ataaagcgat tagacgccgg gctgacagaa caacagaaac aacgaattgt tgaacaggtt
      301 ccctcccggc tacgcaaact ttaccacacg ggttttaaat acgaatcttc ccgccagttc
      361 tgttcaaaat ttgtctttga tatttataaa gaggcgctat gtattccggt gggtgaaata
      421 gagacgtttg gacaactgtt aaatagcaat ccgaatgcaa aactcacttt ctggaaattc
      481 tggttcttag gttctattcc gtgggagcgt aaaaccgtca cgccagccag tttgtggcat
      541 catccgggtt tggtgttgat tcacgcggag ggagttgaaa cgcctcagcc tgaactgacc
      601 gaggcggtat aacttaacgc agtcgccctc tcgccaggtt cagccgcgat tcgctcatct
      661 gcattgcatt ctggctgacg tgacaatggg taatcgcgat agctaacgca tcagcggcat
      721 ccgcttgtgg attagcgggc agtttcagca aggtgcggac catatgctgc acctggcttt
      781 tctcggcgct accaatacct accaccgttt gctttacctg acgtgccgcg tattcaaata
      841 ccggcaattc ctgattcacc gccgccacaa tcgccacgcc gcgcgcctgg cccagtttca
      901 gggctgagtc agcgttcttc gccataaaga cttgttcaat ggcgaaataa tcaggctgga
      961 actgggtgat gatttccgtc acgcccgcat agatgagctt cagacgagac ggtaaatcat
     1021 ccactttggt gcgtatgcat ccactaccca ggtaggacag ttgcctgcct acctggcgga
     1081 tgacgccata gccggtcacg cgcgaacccg gatcaatgcc gagaataata gccatcacgc
     1141 gtctccgttt tgctgtttag caggcctcat cagagagtcg ctgcaacctc atcagagatt
     1201 tcaccgttat ggtaaacttc ctgcacgtcg tcgcaatctt ccagcatatc gatcagacgc
     1261 atcagtttcg gtgcggtttc tgcatccata tcagctttgg tggacgggat catggaaact
     1321 tccgcgctgt ctgctttcag acctgccgct tccagagcgt cgcgcacttt acccatttct
     1381 tcccatgcgg tgtagacatc aatcgcgccg tcatcatagg tcacaacgtc ttcagcacct
     1441 gcttccaaag ctgcttccat gatggtgtct tcatcgcctt tctcgaagga gatcacgcct
     1501 tttttgctga acagataggc tacggaacca tcagtaccga ggttaccgcc acatttgctg
     1561 aatgcatgac gcacttcagc aacggtacgg ttgcggttgt cagacagaca ttcaatcatg
     1621 attgccgtgc cgccaggacc gtaaccttcg tagatgatgg tttccatgtt tgcatcatca
     1681 tcaccgccca caccacgtgc aattgcgcgg ttcagtgtgt cgcgggtcat gttgttagac
     1741 agtgctttat caattgctgc acgcagacgc gggttagcgt ccggatcgcc accgcccagc
     1801 ttagccgcgg ttaccagctc acgaatgatt ttagtgaaga ttttaccgcg ctttgcatcc
     1861 tgcgcagctt tacgatgtct ggtgttggcc catttactat gacctgccat aaaaatatct
     1921 ccagatagcc ctgcctgttc aggcagcgtt aatgacaaac tgttcaatcg cctgccggtt
     1981 gctccaggac ttagtgagcg ccgccgcagc aggcgcatca agccacttgt aagccagatg
     2041 ttcagtgaaa acgatctgcc gctcgtgcgg aagcgcaaga cagaaccatg attccgtatt
     2101 acgcgtcacg cccggcgcat agcgatgacg taaatgtgaa aaaatttcaa actctaccgt
     2161 gcgctgacag tcaattaagg tcagttgttc agcgacaaca tcaatggtga cctcttcctt
     2221 tacttcgcgc atggcagctt gcggcgcggt ttcaccctct tccacgctgc cggttaccga
     2281 ctgccagaaa tcgggatcgt cacgccgctg caacatcagc acccgtttcg tatcttgtgc
     2341 atagatgacc actaagatcg aaacgggacg cttatacact ttatccttca cgctgcctct
     2401 tcgttgactg ccttcgctca tcccattcac atagttatct atgctcatgg gagttcactc
     2461 agttgccgcc ttgatgcaac gcgaatgatt tcgtgtattt gagtcatatc agttattctc
     2521 agccttcttc acaacctgaa tgctcagctc agccagtgca gtcgggttag caaagctcgg
     2581 tgcttcagtc atcagacacg ctgccgccgt ggttttcggg aaggcgataa cgtcacggat
     2641 attgtcggtg ccagtcagca gcatggtcag acggtcaaga ccgaatgcca gacctgcgtg
     2701 cggcggagta ccgtatttca gggcgtcgag caggaagccg aatttctcgc gctgttcctc
     2761 ttcgttgata cccagaatac caaacaccgt ctgctgcata tcaccattat ggatacgtac
     2821 tgaaccaccg cccacttcgt aaccattgat gaccatatcg taagcgttcg ccaccgcatt
     2881 ttccggtgca gctttcagtt ctgcagcagt catgtctttc ggtgaggtga acggatggtg
     2941 cattgccgtc aggccgcctt caccgtcgtc ttcaaacatc gggaagtcga taacccacag
     3001 cggtgcccat ttgctttcgt cggtcagacc aaggtcttta cccactttca ggcgcagtgc
     3061 gcccatcgcg tcggcaacaa ttttcttgtt gtcggcaccg aagaaaatca tatcgccatc
     3121 ttgcgcggca gtacgatcca ggatggcttc gatgatttct gcattaagga acttcgctac
     3181 cgggctgttg ataccttcca gacctttcgc gcgttcgtta actttgatgt aagccagacc
     3241 tttcgcgccg tagattttaa cgaagttacc gtattcgtcg atctgcttac gggtcagcga
     3301 tgcgccaccc ggaacacgta gagcggcaac acggcctttc ggatcgttcg ccggccccgc
     3361 aaatactgca aactcaacag atttcagcag atcggcaacg tcggtcagtt ccatcgggtt
     3421 acgcagatcc ggtttatcag aaccataacg gcgttctgct tccgcaaagg tcattaccgg
     3481 gaaatcgccc agatccacgc ccttcacttc cagccacaaa tgacgcacca gcgcttccat
     3541 cacttcacgc acttgcggcg cggtcatgaa agaagtttcc acatcgatct gagtaaattc
     3601 aggctgacga tcagcgcgca ggtcttcgtc acggaagcat ttaacgatct gatagtaacg
     3661 gtcaaaaccg gacatcatca gcagctgttt gaacaactgt ggggattgcg gcagtgcgta
     3721 gaatttacct ttgtgcacac gagaaggcac caggtagtca cgcgcgcctt ccggcgtggc
     3781 tttggtcagc atcggagttt cgatgtcgag gaagccgtgg tcatccataa aacggcgcac
     3841 caggctggtg attttagcgc gggttttcag gcgctgagcc atttccggac gacgcaggtc
     3901 gaggtagcgg tatttcagac gcgcttcttc ggtgttgacg tggttagagt caagcggcag
     3961 aacatctgcg cggttgatga tagtcagcga ggacgccagc acttcgattt cgccggtcgc
     4021 catatcgcgg ttaatatttt tttcgtcacg cgcacgtacg gtgcccgtga cctgaatgca
     4081 gaactcatta cgcagttcag aggccagctt taacgcgtcc gcacgatccg gatcgaaaaa
     4141 tacctgcacg ataccttcgc ggtcgcgcat atcgatgaag atcaggctac caagatcacg
     4201 acgacggttg acccaaccac acagagtcac ctgctgcccc acgtgggaca aacggagctg
     4261 tccacaatat tctgtacgca tgagatatcc cttaacttag ctgccggcgg atgccccctg
     4321 ctgcgcaggt gaccaagtcg cagcgttagc tgtatgtcac aactgaatga aaaaaggcgg
     4381 ctattatact ggaaattctg ccgcaccgta agagcctggc ccgcgctgga acgcctcgtt
     4441 accactttat atcgggcctg aaatcagact ctacgccagt ttgctataaa ggtgttgccc
     4501 gaactcataa aaattaacaa aatttgtcgt tccgccatcg gctaatcgca ttaaggtgtg
     4561 aggcacgatt ttgttttttc aggagtcatt atgcttgaac ttaatgctaa aaccactgcg
     4621 ctggtggtga tcgatttaca agaaggcatc ttaccttttg ccggtggtcc acatactgcc
     4681 gatgaggtag ttaatcgcgc cgggaagctg gcggcgaaat ttcgcgccag cggtcagccc
     4741 gtgtttctgg tgcgcgttgg ctggtctgcc gattacgccg aagcattaaa acagccggtt
     4801 gatgccccct cccccgctaa agtgttgccc gaaaactggt ggcaacatcc agctgcatta
     4861 ggtgcaactg acagtgatat cgaaatcatc aaacgtcagt ggggtgcgtt ttacggtacg
     4921 gatctggagt tgcaactgcg ccgccgaggt atcgatacaa tagtgttatg tgggatctcg
     4981 accaatatcg gtgttgaatc caccgcccgc aatgcctggg aacttggctt taatctggtg
     5041 attgccgaag acgcctgtag tgccgctagc gccgagcagc acaataacag catcaaccat
     5101 atctacccac gcatcgcccg tgtgcgtagc gtggaagaga tccttcacgc gttatgattt
     5161 acatcggtct accgcaatgg tcgcatccta aatgggtgcg gttggggatc accagccttg
     5221 aagagtatgc ccgccacttt aactgcgtgg agggcaacac cacgctttac gccctgccga
     5281 aacccgaggt tgtcctgcgc tggcgtgaac agaccacaga tgacttccgc ttctgtttta
     5341 agtttccggc gaccatttcg catcaggcag cattacgaca ttgcgatgat ttagtgactg
     5401 aatttttgac ccgcatgtca ccgttggctc cgcgcattgg gcaatactgg ctgcaactgc
     5461 ctgccacatt cggcccacgg gagctgcctg cgctttggca ttttctcgat tctctccctg
     5521 gcgaatttaa ttatggtgtg gaagtccgcc atccacagtt tttcgccaaa ggggaagagg
     5581 aacaaacgct taatcgcggt ttacatcagc gcggcgttaa tcgggtgatt ttagacagcc
     5641 gcccggttca tgcagcatgt ccacacagtg aagctattcg cgacgctcaa cgaaaaaaac
     5701 ctaaagttcc ggtacatgct gtactgacgg cgacaaatcc gctgatccgt tttatcggta
     5761 gtgatgatat gacgcaaaac cgggaattat ttcaggtctg gttacagaaa ttagcgcagt
     5821 ggcatcagac cactacgcct tatctttttt tacatacgcc agacattgcc caggccccgg
     5881 aactggtaca taccctgtgg gaagacttac gtaaaacgct tccagagatc ggagcagttc
     5941 cggctattcc acagcaatct tctcttttct gaatttgcca cctatcatag acaggtgcca
     6001 tcggccattt taaagggagt ttgtatggta agcgcgctat atgccgtttt aagtgcgttg
     6061 ttattaatga agttctcttt tgatgtcgtt cgcttgcgtt tgcagtaccg cgttgcctat
     6121 ggtgacggtg gttttagcga actgcaaagc gctattcgca ttcatggtaa cgcggtggaa
     6181 tatattccca tcgcgattgt gctgatgctg tttatggaaa tgaatggcgc agaaacctgg
     6241 atggtgcata tttgcggcat cgttttgctt gctggtcgtc tgatgcatta ttacggtttt
     6301 catcaccgtc tgtttcgctg gcgacgttcc ggcatgagcg ccacctggtg tgcgctgttg
     6361 ctgatggtgc tggcgaatct ttggtatatg ccctgggagt tggttttctc cctgcgttag
     6421 cgcacaatac gccactttct ttttcccgga tttttacgtt atgtctcacc gcgacacgct
     6481 attttctgcc cctatcgcca gactgggcga ctggaccttt gatgaacggg tagctgaagt
     6541 cttcccggat atgatccagc gttccgttcc cggctattcc aatattattt ccatgattgg
     6601 tatgttagcc gagcgcttcg ttcaacctgg tacgcaggtt tacgatctgg gttgttctct
     6661 gggcgcggcg acgctctcgg tgcgtcgcaa cattcatcat gataattgca aaattattgc
     6721 catcgacaac tccccggcga tgattgaacg ctgccgtcgt catattgacg cctataaagc
     6781 ccctacgcca gtagacgtca ttgaaggtga tattcgcgat atcgccattg aaaacgcatc
     6841 gatggtggtg ctgaatttta ccctgcaatt cctggaacct tccgagcgcc aggcattact
     6901 ggataaaatt tatcaaggac tgaacccggg cggcgcgctg gtgctttcgg aaaaattcag
     6961 tttcgaagat gccaaagttg gtgaactgtt gttcaacatg caccacgact ttaaacgtgc
     7021 caacggttac agcgaactgg agatcagcca gaagcgcagc atgctggaaa acgtgatgct
     7081 gaccgattcc gtggaaaccc ataaagcacg cctgcataaa gccggttttg agcatagcga
     7141 gctgtggttc cagtgcttta actttggttc actggtggca ttaaaagcag aggacgctgc
     7201 atgatcgact ttggtaactt ttattctctg attgccaaaa atcatctttc acactggctc
     7261 gaaacgctgc ccgcgcagat tgctaactgg cagcgcgagc agcagcacgg gctgtttaag
     7321 cagtggtcca acgcggtgga atttctgcct gaaattaaac cgtatcgtct ggatttattg
     7381 catagcgtaa ccgccgaaag cgaagagcca ctgagcaccg ggcaaattaa acgcattgaa
     7441 acgcttatgc ggaacctgat gccatggcgc aaagggccgt tctcactgta tggcgtcaac
     7501 atcgataccg aatggcgttc cgactggaaa tgggatcgtg ttctgcccca tctttctgat
     7561 ttaaccggac gcaccattct ggatgtcggc tgcggcagcg gttatcacat gtggcgcatg
     7621 attggcgcag gcgcgcacct cgcggtgggg atcgacccta cgcaactgtt tctgtgccag
     7681 tttgaagcgg tgcgtaaact actgggtaac gatcagcgcg cacatttgtt accgttaggt
     7741 attgaacagc ttccggcact gaaagccttt gataccgtct tttcgatggg cgtgctttac
     7801 catcgccgtt cgccgctgga gcatctctgg cagttgaaag atcaactggt gaatgagggt
     7861 gaactggtgc tggaaacgct ggttattgat ggcgatgaga acacggtgct ggtaccaggc
     7921 gatcgttacg cgcaaatgcg taatgtctat ttcatccctt ccgcgctggc gctgaaaaac
     7981 tggctgaaga agtgtggttt tgtagatatc cgcgttgtgg atgtgtgcgt taccactact
     8041 gaagagcagc gacgcactga atggatggtt accgaatctc tgtccgattt cctcgaccca
     8101 catgatccga gtaagacagt tgaaggttat cctgcgccta aacgcgcggt gctgattgcg
     8161 cgcaagccgt aaaggtctgg cattactgcc ggatgcggct tgaatgcctt atccggccta
     8221 caaacccttg ctaattcaat ctattgcagg gactatgtag gcctgataag catagcgcat
     8281 caggcagttt tacgtttgca taacctcagc gcccgtttcc gggcgctatt cacttcttac
     8341 gcctgaacag caggcttatc aaatgccgtt aacttcggtg cgttacccgt atatttttcg
     8401 atatacacca gcgcagagtt accggcacag gcatttgcca gctgcgagct ggggatatcc
     8461 gccgttaaca cgttcgcact gccgttttta cacaagccgt tttccagatc tggccatgca
     8521 ccttcatgca cgcataccac gccttttttg atcccgtcag tcaccaccgc gcctgtcaaa
     8581 atctgaccac gtttgttcca gacacgcacc agatcgccat tcgcaatacc aaatcgagca
     8641 gcatcttcgg tgtgaatagt gatcggttca cgatcggcaa ccgcatattt tttacgcagt
     8701 tccgcatagt taagctgact atgtaaacgg tgtgccggat gtgcggtcag aagctgcaac
     8761 tgcttctcgt cggcggtacc cttccactca tcaggcgcaa gccaggttgg atgtgccggg
     8821 caatccttat agccaaattt ttccagcgtt ttggagtaaa tctcaatttt gccgcttggc
     8881 gtacccagcg catttttcac cggatcggcg cggaaatcag cataacgaac gtactgttcg
     8941 ttcttctcgc tgcggcgcat ttcgatcagt ttattttgct gccagaaggc attaaacatc
     9001 ggcatagtca cgcgttgcgc acgagcacct ttctgagcgg catcatagaa aaatttcagc
     9061 cacgccattt catctttacc ttcggtatag atctcttttc cgccaggttt cagtaattca
     9121 gcaagatcgg caaatacgtc aaaatcgtta cgcgcttcaa attgcggagc aacagcctgc
     9181 ttcatcggca caatatgctg gttgctgtaa tcaccggtca tcgtcaagtc attgcgctca
     9241 aacgatgtgg tgatcggtaa tacgatatct gcatgtttag cagctgcggt ccagtagcat
     9301 tcagaaacga cgatcatctc cggtttctgc catgctttga tcagacgatt agtatcctgg
     9361 tgatgggtaa agttaccgcc acccgcccac cagatcattt tgatattcgg atacgtttgt
     9421 tctttaccgt tatgctgata tttgccacca ggattttcca gcgcatcgac gatacgggca
     9481 acacgaatcg ccgtcattcc gccatcatca gcggcctcgc tggcctgtcc ggcaatcgcc
     9541 gcagacattt ccggcagcac gccgccaaca cgcgtcggat tgccaccgtt ggagtaatga
     9601 taagagaaac caaaaccgcc gcccggtgta ccaatctgcc ctaacatcgc cgccagcgtc
     9661 accagcatcc agtgtttctg ttcaccatat tgctggcgct gaattcccca gccagccatc
     9721 agcattgtgc gattcgccgc catcagttca gcgagtttga ctatctgcgc ttccggtacg
     9781 ccagtaattt ccgccgccca ggccgcgctc ttcggagtat tatcgctctt acctgtcaga
     9841 tactcttcaa actgcggata cccggtggtg tatttttcaa ggaacacttt atcgtgttta
     9901 ccttgtgtca tcagggtatg cgcaatccct aacatcagtg ccacgtcggt gcccatattc
     9961 ggcgcgatcc aggtggcatt atcaccaaag aactcgatgg tttcggagcg gataggatca
    10021 atggcaatca ctggtttgcc agattttttc agctgatgga agtattccag cccttgctca
    10081 tcggtactgc tccaggcaat ttttaaggta ttcagcgggt tcatccccca cagcaccaca
    10141 gcctggctgt tttccagaat cagcggccag gaggtctgct gttcatacac ctctacagaa
    10201 ccgaccacat gcggcatgat cacctgtgct gccccggttg aataatcgcc gctatgcccg
    10261 gaataaccgc ccgccaggtt catataacgt tgcagtaagg tttgcgcttt atgcaacacg
    10321 ccagaagagc gccagccgta tgatccggca aaaatagcag atggtccgtt agctttgcga
    10381 atgcgatcgt gttgctcatg aatcagtttt aatgcttgct cccagctcac ctgtacataa
    10441 gtatcttcgc cacgaccttt cgccggttgc agtggattat cgagatagct ttttcttacc
    10501 atcggatgct gaatacgtgc cgtggtgtgt acctgatccg ccgccgtaga ctgtaaggag
    10561 ttcggtatgg ttttcgccag cgcgcctgtt gaagaaacaa tcttgccgtc cttcacttct
    10621 acgttcatcg ctccccaacg tcccgcggtg aggattttac cgcccttctc ttctgcccat
    10681 gcggggagcg gtgctgccga tgtcaccacc agcgctccag cggcaatacc gctgtgttta
    10741 ataaattcac gtcttgttaa tgtcataact tcctccctga tcaacgagga tcactgtttc
    10801 tcggtaatat ctttggcgtt gtactggaaa taccgcgtta aaatgtccag ttcgttttcg
    10861 ctcatgctgg ttcgcgcacc cattcctttg gcaatggacg gccatgcatt gacggtgtaa
    10921 tggtcagcgg caataggcgc atggcaacca gcgcaatagg tatcgtcaag tttttcagcg
    10981 tattgccata gcggtttacg gtccgctaat gcgggatcgg taagcgcacc ctgtaaagac
    11041 gcctgacgcc attgattgcc gtattcgtca gcctgccatt ccccgtttac cgtgagcgcc
    11101 ttgatacctt cttcacttaa tgtggctagc gccagccgtt gacctgccgc caggtagagc
    11161 gtgttttcac tgccctgcat ttgataaccc tgcaacagaa cgatcggctg tttgccactg
    11221 gcatcaacga cggtgagatc ggtaccagga ttcacggtag ccagctcgcc tatgcgagaa
    11281 gttttgaaag gataaatatg tgcgccatta gtcactgaag tagcggcctg actttccagc
    11341 tcatgcgccg cgttgtcatc catttttatt tctggcggaa aatgggcaat gcctttatga
    11401 caatcgatac aggtttcgcc gcccttttgt gctttgttat gcattttctg cgcagattca
    11461 ctttgcgagg caatatccat ggcatcaaaa gaatggcaac tacggcacgt tgcagagtca
    11521 gttgctttta attctttcca tactgtttcg gccatttcct ggcgatgagt ttcgaactta
    11581 tcgtcactgt ctattttgcc gctaacaaat tcatgataaa tatctttaga tgcttttaat
    11641 ttagcaaata aataatccat ccctgacttt ggaatatggc aatctgcaca ttccgcacgt
    11701 atccctttct ggttcgaaaa gtggacagtt ccctgatatt cctcaaaagg tttactcatc
    11761 gagtggcagg aaaggcaaaa tgctgtatcc gacgttttat gtaaggcttt ttgcgccagc
    11821 aataacccgc cgccaccaac cacaaccgct atcagcaaaa acaataaccc aatgcgtttt
    11881 ttccctcgca tagattcctc taaataaatt ccagaacacc gaaatcattg tataattaaa
    11941 tacacaacga attaaccgct tgcataactc ggcacaacac tgcctgaaat aatcgataaa
    12001 gaatatgatt tattacaatg taatcattaa ttgctaagga ataacccagt tgctatttag
    12061 aatatatatt tatcagtttt agcaatttaa atcccataat caatgtgaat ataaaaaata
    12121 ttattagcac aacgctatgt tcactgtcac ccgctcaaca gatcacggtt atcgttcgtt
    12181 ttttatactg ctcagggata aaaaaaggcc cctgttgaaa ttgcaggggc ctggtacgag
    12241 caagcatcat attgggcgac atgatgcaac ggtaaaaatc atttggcctg atggcgttca
    12301 atgattcctt tcatttcagc aaccgccgcc ccgtctacga tataacgcga atactcgtcc
    12361 gcgtgttcat ctgatgacat ggacaatcct tgattacgat aacgcatcgg tgaggcttgc
    12421 cacgctcccg cggagctatg gacttccagc actccggcat cgaggaagtg gtgcaggttt
    12481 tctgcacgga ctcctgctcc ggccataatg attggagcat cacgatgggc aataagttcc
    12541 ataatttttg ataaaccttg cagtgcgtct gatttttgcc ctgatgtcag tacccgggca
    12601 atgcccaatt ccgcaagatt attgagtgta tataaagggt tagcgcacat atcgaaggcg
    12661 cgatgaaaag tcactgccag cggaccggca gcagccatta ttttttccat tcgtggcata
    12721 tcgacattcc cgtcaacatc gagaacgccc gtcaccagtc caggaaaacc taattcgcgg
    12781 accgtgcgca catcctcaag aatggcggca aactcaccgt cgctgtagca aaaatcacca
    12841 ccgcgtgggc gaattatcgg atgcacaggg atcgtcaccc gctggcgcac ggatttcagt
    12901 acccccaacg acggcgttaa gcccccctct tttggggctg cgcataattc aactctgtct
    12961 gcgccgtttt gctgcgccgt tagtgcacat tccatgctgt aacagcaaat ttccagtaat
    13021 gccatttttg ctccttaatt acgctgactg ctcgctggca acgatctctt caatggacca
    13081 cggatgaaac ttaatagttg ttttgccatc ggtgaccgcc agcgtcggat tcggtaatcg
    13141 ctcatgctca ccttttggtg aactggtttt aactgagatc cccggtaggc tgagtccttc
    13201 atcagaaagc aatgccagcg cacgggcatt cagcgtttct ggatcacccg gcagaacgat
    13261 ttcaatatgt tcccaacctt cgtgtgggta acgtttttcc ccgggccacg gtagctccac
    13321 aatagaaaac tgccagtgcg caacctgtac cggttcatgc aatttaaaca gacaaatcgg
    13381 tctgccattg atcatatttt ctgacaaaag ctcgccacac tgttcaaacc cgcgacgcca
    13441 gcgttcagca gtggcgtttt gatggcaacg caaagaaatg tgatcggcag tcagcggagt
    13501 gatattcaac cccagacgga gggaaagttc atctaatgcg tgggtaaatc gcggtaaatc
    13561 cgatgcaata tcctgcagct cgtcgataga ttgccagttc gccataatca ctcttcgtct
    13621 ttcagtaaaa gcgttaattt accctgttgc cctgcgccaa ccaaccgctg atttcacgcc
    13681 gcttctgatg caatagtgaa aacggcaata cgccacgcgc acgttgctga cgaaaacagc
    13741 catttgcagt atactcccgc cctaatttct ttaactggtg cgggcaattt ttgctcgctt
    13801 catcaatgta aggtattccg gtgaatattc aggctcttct ctcagaaaaa gtccgtcagg
    13861 ccatgattgc ggcaggcgcg cctgcggatt gcgaaccgca ggttcgtcag tcagcaaaag
    13921 ttcagttcgg cgactatcag gctaacggca tgatggcagt tgctaaaaaa ctgggtatgg
    13981 caccgcgaca attagcagag caggtgctga ctcatctgga tcttaacggt atcgccagca
    14041 aagttgagat cgccggtcca ggctttatca acattttcct tgatccggca ttcctggctg
    14101 aacatgttca gcaggcgctg gcgtccgatc gtctcggtgt ttctacgcca gaaaaacaga
    14161 ccattgtggt tgactactct gcgccaaacg tggcgaaaga gatgcatgtc ggtcacctgc
    14221 gctctaccat tattggtgac gcagcagtgc gtactctgga gttcctcggt cacaaagtga
    14281 ttcgcgcaaa ccacgtcggc gactggggca ctcagttcgg tatgctgatt gcatggctgg
    14341 aaaagcagca gcaggaaaac gccggtgaaa tggagctggc tgaccttgaa ggtttctacc
    14401 gcgatgcgaa aaagcattac gacgaagatg aagagttcgc cgagcgtgca cgtaactacg
    14461 tggtaaaact gcaaagcggt gacgaatatt tccgcgagat gtggcgcaaa ctggtcgaca
    14521 tcaccatgac gcagaaccag atcacttacg atcgtctcaa cgtgacgctg acccgtgatg
    14581 acgtgatggg cgaaagcctc tacaacccaa tgctgccagg aattgtggcg gatctcaaag
    14641 ccaaaggtct ggcagtagaa agcgaagggg cgaccgtcgt attccttgat gagtttaaaa
    14701 acaaggaagg cgaaccgatg ggcgtgatca ttcagaagaa agatggcggc tatctctaca
    14761 ccaccactga tatcgcctgt gcgaaatatc gttatgaatc actgcatgcc gatcgcgtgc
    14821 tgtattacat cgactcccgt cagcatcaac acctaatgca ggcatgggcg atcgtccgta
    14881 aagcaggcta tgtaccggaa tccgtaccgc tggaacacca catgttcggc atgatgctgg
    14941 gtaaagacgg caaaccgttc aaaacccgcg cgggtggtac agtgaaactg gccgatctgc
    15001 tggatgaagc cctggaacgt gcacgccgtc tggtggcaga aaagaacccg gatatgccag
    15061 ctgacgagct ggaaaaactg gctaacgcgg ttggtattgg tgcggtgaaa tatgcggatc
    15121 tctccaaaaa ccgcaccacg gactacatct tcgactggga caacatgctg gcgtttgagg
    15181 gtaataccgc gccatacatg cagtatgctt acacgcgtgt attgtccgtg ttccgtaagg
    15241 cagaaattaa cgaagagcaa ctggctgcag ctccggtaat catccgtgaa gatcgtgaag
    15301 cgcaactggc agctcgcctg ctgcagtttg aagaaaccct caccgtggtt gcccgtgaag
    15361 gcacgccgca tgtgatgtgt gcttacctgt acgatctggc tggtctgttc tctggcttct
    15421 acgagcactg cccgatcctc agcgcagaaa acgaagaagt gcgtaacagc cgtctgaaac
    15481 tggcacaact gacagcgaag acgctaaagc tgggtctgga tacgctgggt attgagacag
    15541 tagagcgtat gtaatcgatt tttcgtgaga gtaaagcctg atcagggtta cctgatcagg
    15601 cttttttatt gccatctaaa tgtattctga aaatggacat gccattgttt tctgactgtt
    15661 ggataagagg ccagaagcgt aatatccggc cccagggaaa cgataacggt tgaatttaag
    15721 gaataccgca gtgtttaaat ttcttgtatt aacattaggc attatctctt gccaggctta
    15781 cgcagaagat acggttatag taaacgacca tgacatttca gccatcaaag attgttggca
    15841 aaaaaattca gatgatgata ctgacattaa cgtgatcaaa tcatgcctgc gacaagaata
    15901 caatctcgtc gatgcgcaat taaataaagc ctatggtgaa gcttatcgtt atatagaaca
    15961 agtgccacgc acaggtgtaa aaaaacctga taccgaacaa cttaacttgc ttaaaaaatc
    16021 acagcgagcc tggctggatt ttagggacaa agaatgtgaa ttaatccttt caaatgagga
    16081 cgttcaggat ttaagtgacc cttattctga atcagaatgg ctctcatgta tgatcataca
    16141 gaccaatacg cgaactcgcc agttgcagct ataccgtaac tctgaagatt tttatccaag
    16201 ccctttgaca agaggataat tcacatcttt ttggcatgtt ttgttgcaag ctattcctga
    16261 taaataattg caacaagaca tcgagccttt ttcactgagt tattaaacat actcgcgagc
    16321 gcgtaatttt tttgtccttc agcgataatt cacaatcact tcattacgtt gtacctttag
    16381 cggtggaatt aaccgaccac cgcctggcac ttcccagata aatcgcaacg gttctgctgc
    16441 cgctataccg gaaaaggcca cggtggttcc gctctgcccc tctaattcga cacaacgaga
    16501 ttgcgaacac aagcgaaccc gcagtcctga aggtgtcggg ccgataagct gataacgcca
    16561 cgctaccagc gtcatcaatc ctgaagcagg ttgtcgcgta gaaagaggcg cagacgacat
    16621 cgactcaccg cgatgattta gcgtaattcc tacactactt gcctgccaca tcccctcccc
    16681 ggcggcttgc accagcagcg gaaacaataa tattgctaat aaggttctca ttatttgccg
    16741 ccaattgtcg ccgtcatgcg gatatgtcgg ttatcagaca gttccagatt cgacaggacc
    16801 actaactgcg gcaagctgcg gcgcaggaag cgagacaata atggtcgcgg cgcgtggttc
    16861 accaacaata ctggcggcgc acccagcatc tcctgacggg atagcgcttc ctgagtttgc
    16921 gccagtaaac gatccgccag ccctggctcc agtcctcccc cgccctgcag cgcctgtagt
    16981 aacaaacgtt ccagcggtgt atcgaggcca ataacatgga cttcatcttt gccaggaaac
    17041 cactgctggg taatcgcccg tcccaacgcc acgcgcacga cggcggttaa ttcatgtgga
    17101 tcgctttgga tgggcgcatg ttccgccagc gtttcgagaa tggtgcgcat atcgcgaatc
    17161 ggtacttttt catcgaggag attttgcagc actttatgca gtgtggtgag cgtgacgacg
    17221 ccaggaacga gatcttccgt cagctttggc atctcctggg cgacgcgatc caacagctgt
    17281 tgcgcctcct gacgaccaaa cagctctgcg gcatgctggc taatgaggtg gttaagatgc
    17341 gttgctacca ccgtgctggc ctcaaccact gtgtacccct gaatctgcgc ctgttctttt
    17401 agcgcacttt caatccagat agcattcagg ccaaatgccg gatcgacggt cgcctcacca
    17461 ggtaacgtcc cggcagcggt tccagggtta atcgccagcc agcgccccgg ataagcatca
    17521 ccactgccaa tctccacgcc tttcatcaaa atgcgatagc gggcaggttg cagctccata
    17581 ttgtcgcgaa tgtgcaccac tggcggcaga aatcccatct cctgggcaaa tttcttgcgg
    17641 atactgcgta tacggcccaa caactcacca tcctgctgga aatcgaccat cgggatcagt
    17701 cgataaccca cttccattcc cagagaatct tccagttgta catcgttcca cgtcgcttcg
    17761 acaacggtat tattctctgc catttttacc ggtttgggtt cgacaggcgc tttttgttcg
    17821 cgtccgcgta tccaccaggc cagccccagc aatccggcag tgaacagcaa aaataccagg
    17881 ttcggcattc caggcaccag gccgagtaaa ccgagcacgg cggcgcttaa caacataacg
    17941 cttgggttac tgaaaagctg attcaccatc tgctcgccaa catcctgatc ggtgctgaca
    18001 cgcgtaacga tgacccccgc ggcggtagaa atcaccagcg ccggaatttg tgccaccaga
    18061 ccgtcgccaa tggtcaatag cgtataactt tccgccgcgt gtcccatgct catgccatgt
    18121 tgcagcacgc cgaccagcaa cccgccgaca acgttaatga ccatgatgag gatcccggcg
    18181 atggcatcgc cgcgaacaaa cttacttgcc ccgtccattg agccgtaaaa atcggcttcc
    18241 tgagtcactt cggagcggcg ttttttcgcc tcatcttcac caatcaatcc agcgttaagg
    18301 tcggcgtcaa tcgccatctg cttacccggc ataccatcga gaacaaagcg cgcacccact
    18361 tctgcgatac gcccggcacc tttggtaatg accataaagt tgatgatcac gagaatgaca
    18421 aacactacga taccgatagc gaaattgcca ccaacgagga agtgaccgaa tgcttcgacc
    18481 accttcccag ccgccgccgc gccggtatgc ccttccatca aaatgatacg ggttgaagcc
    18541 acgttaagtg ccagacgcaa caacgtagta aacaacagaa tggtcggaaa cgcagcaaac
    18601 tcaagcgtgc gctgggtaaa catcgccacc agcaacacca tgatcgacaa ggcaatattg
    18661 aaggtaaaca acaggtcgag tatgaatgcg ggcagtggca gcaccatcat cgacaagatc
    18721 aacaggatca aaatcggtcc ggcaaggatc tgccattgtg tcgatttcag gtttgcgggc
    18781 aggcgcagca tcgcggccag attactcatg ggtcggtttc tcgttaataa aatccagggc
    18841 ttccggcacc ggaagatgag taggttgtac agggcgctgt ccaccagcca gacgccagcg
    18901 tttcagttgc cagacccagg ccagcacttc cgccaccgcg gcgtacagtt gacccgggat
    18961 ttgttgacca atctccgcat gtcgatacag cgctcgcgcc agcggcggcg cttcaagcgt
    19021 cgggacgtta ttttcagcgc caatttcacg aatgcgcagc gcgaccagcc ctgcaccttt
    19081 agcgaccact ttcggtgcgc tcattttgtt ttcgtcatac tgcaacgcta ccgaatagtg
    19141 ggtcgggtta ttgacaatga catccgcttt cggcacatcg gccatcatcc gacgccgtgc
    19201 agcagctcgc tgcatctgac ggatccgccc tttaacatga gggtcacctt cgctttgttt
    19261 gaactcatca cgaatatcct gccgtgacat acgcagcttt ttcaggtggc tgaatatttg
    19321 gaaaaagacg tcaaatccca ccattggaat gacaccaagc accaccagca gtgcgcatag
    19381 ccctaccaaa tccatcgcat tacccatggc ggtaatcgga gactcggcca tcaagcgcat
    19441 catctgcggc cagtgatgcc agagaaaaaa ccccgtcacg ctgccaacca ggatggtttt
    19501 caaaattgct ttaagcaact ccgcgccagt ctgagccgag aacatccgtt taatgcccgg
    19561 tagcgggttg agtttggaaa acttcggctg caaggatttg ccgctaaata ccagccctcc
    19621 cagcatgacc ggagaaatga gcgccaccag caccacgccg ctaatcagcg gcagcagcgc
    19681 cagcatggct tctctgatca gcagaataat ctgcccgagg atcagattcg ggtcattgat
    19741 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
    19801 cacaccgcca aaccagataa cactaacgcc caccagcaaa atcagcagtg aggtcagttc
    19861 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
    19921 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagcgt
    19981 gaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
    20041 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
    20101 cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc
    20161 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacaccgg ctttgctggt
    20221 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
    20281 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
    20341 catccgctta atgacctgcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
    20401 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
    20461 tgttaccagt tcacgggcgt cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
    20521 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
    20581 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
    20641 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
    20701 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
    20761 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
    20821 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
    20881 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
    20941 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
    21001 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
    21061 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
    21121 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
    21181 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
    21241 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
    21301 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
    21361 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
    21421 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
    21481 taccgaaggc cgatgacggt taaccgccgg gccatcgtga attttgattt ggtaatttgc
    21541 gccactacgc gacagctcca tatgccgatc gcccggcgca atataggcat gccccggcaa
    21601 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
    21661 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
    21721 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
    21781 cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa
    21841 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
    21901 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
    21961 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
    22021 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
    22081 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
    22141 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
    22201 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
    22261 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
    22321 tggctaaagt tttcagagtg acccgcaaac agcaatccgt cgggtttaag gagcggaaca
    22381 aagcagcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
    22441 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
    22501 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
    22561 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
    22621 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
    22681 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
    22741 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg acgtgcgtga
    22801 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
    22861 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
    22921 tgaccgaaat ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
    22981 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
    23041 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
    23101 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
    23161 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
    23221 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
    23281 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
    23341 tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgtcc
    23401 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
    23461 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
    23521 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
    23581 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
    23641 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
    23701 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
    23761 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
    23821 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
    23881 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
    23941 ttacgcacat cactcaccgt cccacgcaaa gcctgctgca tggtcttcag gctggcaaaa
    24001 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
    24061 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
    24121 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagcgcc
    24181 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
    24241 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg attcctgcac cggcgcagtg
    24301 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgttg
    24361 tgccagcggg caaaactttt ctccgtctct ttttgcaacg ccctgacgtg ctcgttgccc
    24421 gcagtcatcg ccataaaact tttaaacagc gtggtggatt gcgtcagact ggcgcgcgcc
    24481 gtcgtcatca acgttttaat gtcatccgcc ggatagctaa gtgccgtcag agtgcccgct
    24541 ttgttcagcg cggtactggc ctgtaacatt accgcccgag tttgcgctaa tgccgcacgt
    24601 tgctgattgc tctgctcgac ctgattcagt cgttgcaaat cgtcgcgaaa tgcccaaaaa
    24661 gacatgccgt tactgccaat ctgcaagatc ccgcagagaa tcaaaattaa aaacagcgtg
    24721 gtcgaaattc gaatacgatt aaacatcaac gctccccatc aggcggcaat gaccgcgtta
    24781 gtaaatactc gtcaaaatgt ttcccagttt ggagcttgtt cagcaattcg cagtcgtggc
    24841 tgtgccggtg gttgctcact ggcaggacgg gatggtgttt gcggtttatt ggtgagtggg
    24901 ctggctgcca gacggaacgc ggaaaccgct tgcgttaaac gactcgcctg ttcttccagc
    24961 gcagcggcgg cggcagctga ttcctgcacc agcgatgcgt tctgttgcgt gacgcgatcc
    25021 atttccgaaa ccgccaatgc gacttgatcg atgccacggc tctgttcatc cgatgccgat
    25081 gcaatctcgc ccataatgtc agtcacgcga gtgacagcat tgacgatatt gttcattgtt
    25141 tccccggcgc tttcgaccag caccgaaccg gtatcaacgc gtgagacgga gtcttcaatg
    25201 agggctttga tctcttttgc cgcctgggcg ctgcgactgg caagattacg cacttcaccc
    25261 gccaccacgg caaaaccacg gccctgttca cccgcacgcg cggcttcaac cgcggcattc
    25321 agcgcgagga tattagtctg gaaggcaata ccgtcgataa cgctgataat gtcggcaatt
    25381 ttcttcgaac tatcggcgat ctcatgcatc gttttcacta cgccatccac cactttgccg
    25441 ccgtgctggg cggtgtcgga ggcactttgc gccagttgcg aggcctggcg ggcgttatcg
    25501 gcgttttgct tcactgtcgc ggtgagctgc tccatgctgg cggcagtttc ttccagcgcg
    25561 gatgcctgct gttcagtacg ggaggaaaga tcggtgttgc ccgccgcaat ttcacgggta
    25621 ccggcataga tggcatctga accttcgcgg acatgagtga cggtgtcagt caaagagcgt
    25681 tgcatatgtg aaacgctctg cgccaggtcg cccatttcac tgcgcccgtc aatggtcagg
    25741 gtattcgcca ggttaccacc ggcgatttcg cgaatgtgag caataatttt tgccagcgga
    25801 gtaagcaaca tacggcgaat gccgtaccac gccaccagca gaatcaatac caccaccagc
    25861 gcgataaccg ccagttgcca ctgggcaaat cggtaatcat ctgcgttgtc agtgacgata
    25921 tcgcgataca gtttttcact gctgagggcg tactgagcaa acgcttcgcc cattgcattt
    25981 tgcattccct gggttggctg agcgaaataa gctccagtat tgccataatc aagataatca
    26041 atcagttcag ttaacgctgt gtgatagttt ttatattttt catcaatatt acgactggta
    26101 gcgaccattt caggtaacgg tgccatgctt ttgaattttt tataatgagt cgcggcctgg
    26161 gccaatgttt tcctggcgct atcgagcaat tcaactttgg cgttactttg ttgattggag
    26221 gaatccatca tcatccgcac cgctgaacga ctcaggttaa tgcgcgtttg cagcattaaa
    26281 tcccaggttg acgtcagctc gccctgctgt tcccgtaatt gattggaaac cacaaagctc
    26341 ttctggctat ggtgaaggga agaaaaaaac agactgccgg aaataagctg taacagtgcg
    26401 aataccccca gcaccattac caacagcgtg actacgcgga tacggttaat cataaggcac
    26461 cttcctgaaa acaagttaat ctcgttatcg gcaagaaggg gggaaacttt attgctgatg
    26521 ccacccgccg cgaaattgaa ataaaaaacc cgatgcgcag atcatcgggt tcatttcaat
    26581 tgaggaaatc gggagaatta cgccacttct gacgccgcgc tatctaacag cgccatctct
    26641 tcgctgttca gcagtttttc gatgttcacc agaatcaaca tccggtcgcc cagtgcgccc
    26701 agtccagtga gatattctgt tgaaagcgtc acggcaaact ctggtgccgg acgaatttgc
    26761 tccgccgtca atgaaagcac gtctgaaacg ccgtcaacca cgatgccgac cacccgctgt
    26821 ccgagattca ggacgataac taccgtgttg tcgttatagt ccacatccac ctggctgaac
    26881 ttaattcgta agtcaacaat cggcacaata acgccgcgca gattcgtgac gcctttgata
    26941 aacgctggcg tgttcgcaat ccgtgttacc tgatcgtagc cacggatctc ctgcactttc
    27001 aggatatcaa taccgtactc ttcatcacca agggtaaata ccagaaattc ctggcctgac
    27061 ggctcgctgg ccagctttgt tacattcgtc ataccggtca tattgttacc tttttactca
    27121 ttcaggcggc ggtgttcgcc atacgttgtt cgcggtttat cgcctgcaag gcggagacat
    27181 caacaatcag tgccacgctg ccgtcgccaa gaatggtcgc agcagaaatg ccggggactt
    27241 tgcgatagtt actttcaagg tttttaacca caacctggtg ctgaccaatt aattgatcca
    27301 ccagcaaggc gtagcggcga ccgccacttt gtaagatcac cacaattccc tgggtggctt
    27361 cggttttcgc gcccgcgacg ttgaacactt tccacagttc gacgatgggc agatattcac
    27421 cccgcacttc cagcacccgc tcgccgccgg ccagtggatg cagatcggct tcacggggtt
    27481 gcagtgattc cataacagca ttcagcggca gaatgaaaac ttcatccgca acgcgtacgg
    27541 acatgccgtc gaggatggcc agcgtcagcg gcagtaaaat gcggatcgta gtgccagtac
    27601 cctgcttcga ctggatttcg acatgaccgc ccatctcctg gatattacgt ttaacgacgt
    27661 ccatgccgac gccgcgcccg gagacgtcgg tgacctgctc tgccgtggag aagcctggtg
    27721 caaatatcag catcgcgact tcgtcgtcgc tcatgttttc gctgacagtc aaaccttgcg
    27781 aggccgcttt tgccagaatt cgctcacggt ttagccccgc cccatcgtcg gtcacttcaa
    27841 tgcaaatgtt gccgccctga tgttcggcag acagaattaa atttccgacg ctgtttttac
    27901 ctgcggcgag ccgtttttct ggcagttcaa taccgtgatc gaggctattg cgtaccaggt
    27961 gggtcagcgg gtcgataatg cgttctatca ggcttttgtc gagttcagta gaactgccca
    28021 ccagcgtcag ttctacctgc ttgccgagtt ttcccgccag atcacgcacc agccggggat
    28081 agcgactaaa aacatattcc atcggcatca tgcgaatcga catcactgat tcctgcaaat
    28141 cacgggcgtt acgttgtaac tgccccatgc tggttatcaa atcaccatga ttaaccgggt
    28201 ccagttcgct ggaacgctgg gcaagcatgg actgggtgat aaccagctcg ccgacgaggt
    28261 taattaattg atcaaccttt tctaccgcta cacggatgct ggtggattca ttgctgcgcg
    28321 tcgttttttc ccgctccacg cggccggttg gcgcttgttc ggctgccagt ttaagcactg
    28381 gtggggtgga tatttttggc gagacttcta ctgtttcaaa ggtaatctga tcggcttcaa
    28441 tcacaaaaca gagtaccgct gtgatgtcat cttcggcgat gtcgcccggt aatattgccg
    28501 agagcgaatc cgcccctttc accacgtcag ttaacgttgt cagatgtccc agttcttctt
    28561 ccagcaggtc gacttccccg gccttcaggc gcgaaaggat aattcgtcgc ggcgactgac
    28621 tgcgactctg ctcatcttgc ggttcacttt tggcaaccac acttaatcgg gtcactgcgg
    28681 atggcgtttc gcctttcgct tctaatgcca gttgacgcaa ggcctggcag atataatcga
    28741 agctggcggc atccggctct tgcgactgtt tataagcgtc gagctgttct tgcatgatgt
    28801 ccttcgtttc caaaaacaga ttgataatgt cggtgttgag ttgcatctca cctcgtctgg
    28861 cttcatcgag caggttttcc atcagatgcg tggtttcctg caaaacgctg aagccaaaag
    28921 ttcctgcccc tcctttgatc gagtgggcag cccgaaagat ggcattcaat tgttcggcat
    28981 ctggcgcttc cggctgcaaa accagcagat gctgctccat gtcagccaac agttcgtccg
    29041 cttcatcaaa aaatgtctga taaaaatcgc ttatatccat gctcacgctg tcacctcggt
    29101 tcggctgatg gcattgtggg aacactgacc tgtggtgcaa cctcaggttt ttccagggcg
    29161 cttactggct cattctggct ttcggcgttt tcatgcaaaa tggcctgttc ggcttgtttg
    29221 ttcagtacca gcaggctgat gcgacggttg acggcatcat caggtccgcg atcgcttaag
    29281 cgcatcgttg ccgccatgcc gacgacacgt aacactttgc cgctatccaa ccctccgacc
    29341 atcagttcgc ggcgggatgc attggcccga tcggcagaaa gctcccagtt gctatatcct
    29401 ttctcaccgc tggcgtaggg gaaatcatcg gtatgacctg aaaggctaat acggttggga
    29461 ataccgttca gtacaggcgc aatggcacgc agaatgtcgc gcatataggg ttcgacatcg
    29521 gcactgccgg ttctaaacat cgggcgattc tggctatcga tgatctgaat acgtagacct
    29581 tcctggacca gatcgatttt gagatgggga cgtaacgccc gcagtttcgg atcggactct
    29641 atcaactggt cgagatcacc ccgcaatttc cgcaatcgac tttgctccat gcgttttttc
    29701 agctcttcga tgttcggctg cttattcact tccccctggc tttgggtgta atcatcacca
    29761 ccgccgggaa ttgggctttc actattagaa atgcgatcgc cgcccgtaac cgcagtcgcc
    29821 agtggagtcc ggaagtactc cgcaatctga atcagctctt ttgggctgga gatggagatc
    29881 agccacatca ccagaaaaaa ggccatcatc gcagtcataa agtcggcata agcaatcttc
    29941 cacgatccat gtgctgcccc gtggcttttg gctttgcgtc gtttgacgac aataatcgga
    30001 tgcgcttgat tcttcatgct tcctcggttg tcatctgttg ttgcggattt ttcaccgcac
    30061 gcacatgctc ttccagttca ataaacgacg gacgttcgct ggaatagagc gttttgcgac
    30121 caaactcaac ggcgataggc ggtgcgtaac cgttcagatt agaaagcaga gtgactttga
    30181 cgcactgcat cattttgctg gtttcggcgc ttttctgacg taaaacactc gctaatgggg
    30241 aaataaatcc gtaagccaat aaaatgccga ggaaagtccc caccatcgca tgtgcgataa
    30301 gcgcacccag ctcggcggca ggacgatcgg ctgaacctaa cgcgtgaacg acccccatta
    30361 cagccgcgac aataccaaac gccggaagtg agtccccgac cagcgccaga ctgttcgccg
    30421 ggacttctgc ctcgctttcg tgcgtctcaa tctcttcatc catcagggct tcgatttcga
    30481 aggtgttcat atgaccgctg ataatcagac gcagataatc gacgataaaa tcaagcatga
    30541 cgctatccgc gaggatgcgt gggtagctgg cgaagatctc gctctcacgg ggattttcaa
    30601 tatcacgttc cagcgaaaac atccccatct gccgcgattt cgccatcaac cgataaagca
    30661 gagccagcag atccatatac attgctttgg tgtatttgga gcgacgaaac agcaacggca
    30721 gcgccttcag cgtgccttta atcgctttgc cattattgcc gacgataaac gacccaatcc
    30781 ctgcaccggc aataatcacc agttcagcgg gttgatagag tgctccaagg cttccaccgg
    30841 tcatcaaata accgccgaaa actgtaccga gaacaaccag gtaacctaat aagataagca
    30901 cgacatcatc cttccactgt tgaccatgac aggatgttca gtcgtcaggc gttaacgcgc
    30961 gattggggca aaaaaaagca gcggtacgtc gttaccgctg ctggaatgtt gcgcctcacc
    31021 gtatcagtta aacagcctgt actctctgtt catccagcag ttgtgggata atatcggcag
    31081 gattctggga aagtttacgt ctttttactg cccgggatgg cggttgacat aagctgcagg
    31141 caaagctgcc aacaggctgg tgagcgtggg taataaaatt gccgccgcag cagttgcagc
    31201 tggaaagttg cagtaatcca ctttcaacaa accgcaccaa tgtccaggca cgggttaatg
    31261 ccagcagtgg tccttcttct gcttgtgggc actgttcaag gtataaacgg taggctttga
    31321 tcaccgcatc gacgccatta cacaaaccgg ttttcagtaa aaactgccat gcattacaga
    31381 acatcgaagc atgaacgttt tgttcccagg tcataaacca gtcggttgag aatggcagca
    31441 tgcctttcgg cggtgggctt ccgcgcagtt ctttataaag ttttatcagg cgtccgcgac
    31501 ttaactgtgt ttcgctttcc agcatctgca aacgagcgcc cagggtgatc aattccattg
    31561 ccagctgaat atcccgcgct tcctgaacaa tgcttttttc actcatgatc aggccctttt
    31621 cttgcgcagc gcttcttcag gctgattaac atcattcagc aagcgtgttg agagcatgat
    31681 gccggtatga atttgctgga gatcgtcaac gcgggaatct tgcgtcaact gagtaatcgt
    31741 ctggtggctg tcaaaacgga agtgacaaac cagttgattg gtttctgcca gcttaaccat
    31801 ttgcggaaga gtcagtgccg ctaacgttgt cgccatttct tcatttatgc cgagacgaaa
    31861 catagcggac gctttgtcct gaacaatcaa acgctgtgca agtagtaaat atgacaagtt
    31921 gatgtcataa atgtgtttca gcaactcgga ggtatgcatt attcccaccc agaataacca
    31981 actttatttt tatgcggttt caccgcaccc cgtgatgtcg ccgggaagcc ccggtaaaaa
    32041 ataattagca ttagaatagt tgcgataagc tgcaataagc agaaccacct ttttggttta
    32101 atatgtcctt acaaatagaa atgggtcttt acacttatct aagatttttc ctaaatcgac
    32161 gcaactgtac tcgtcactac acgcacatac aacggagggg ggctgcgatt ttcaataatg
    32221 tgtgatgcag atcacacaaa acactcaatt acttaacata aatgtataag tcatactttt
    32281 gttttgggtg tatttcaatc tgttaaaaag tttttcgcta cgctagcaag caaaaatgaa
    32341 acaggaataa tcgaaatggg atgttgcgca cagttaaaat aactcaccgt aaataatcat
    32401 ctgctataaa taatcacttt catgcaatac cagataagct atttttaaac agacacttac
    32461 cgcacaacaa actaattaaa tacaaagaaa ggcaacaatc aacttaatgt taat
//
LOCUS       DAZGJW010000045        32386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZGJW010000045 DAZGJW010000000
VERSION     DAZGJW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.045"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(177..305)
                     /gene="rhuM"
                     /locus_tag="ACHKIK_002075"
                     /pseudo
     CDS             complement(177..305)
                     /gene="rhuM"
                     /locus_tag="ACHKIK_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            complement(384..478)
                     /locus_tag="ACHKIK_002076"
     tRNA            complement(384..478)
                     /locus_tag="ACHKIK_002076"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(442..444),aa:Sec,seq:tca)
     gene            771..2153
                     /gene="yicJ"
                     /locus_tag="ACHKIK_002077"
     CDS             771..2153
                     /gene="yicJ"
                     /locus_tag="ACHKIK_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO9083247.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            2163..4481
                     /gene="yicI"
                     /locus_tag="ACHKIK_002078"
     CDS             2163..4481
                     /gene="yicI"
                     /locus_tag="ACHKIK_002078"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO9083248.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGNNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(4534..6243)
                     /gene="yicH"
                     /locus_tag="ACHKIK_002079"
     CDS             complement(4534..6243)
                     /gene="yicH"
                     /locus_tag="ACHKIK_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO9083249.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRINGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDVAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(6364..7755)
                     /gene="xanP"
                     /locus_tag="ACHKIK_002080"
     CDS             complement(6364..7755)
                     /gene="xanP"
                     /locus_tag="ACHKIK_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO9083250.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            8035..9240
                     /gene="gltS"
                     /locus_tag="ACHKIK_002081"
     CDS             8035..9240
                     /gene="gltS"
                     /locus_tag="ACHKIK_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO9083251.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            9243..10109
                     /locus_tag="ACHKIK_002082"
     CDS             9243..10109
                     /locus_tag="ACHKIK_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083252.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(10094..12175)
                     /gene="recG"
                     /locus_tag="ACHKIK_002083"
     CDS             complement(10094..12175)
                     /gene="recG"
                     /locus_tag="ACHKIK_002083"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO9083253.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVHHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(12181..12870)
                     /gene="trmH"
                     /locus_tag="ACHKIK_002084"
     CDS             complement(12181..12870)
                     /gene="trmH"
                     /locus_tag="ACHKIK_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO9083254.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(12877..14985)
                     /gene="spoT"
                     /locus_tag="ACHKIK_002085"
     CDS             complement(12877..14985)
                     /gene="spoT"
                     /locus_tag="ACHKIK_002085"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO9083255.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(15004..15279)
                     /gene="rpoZ"
                     /locus_tag="ACHKIK_002086"
     CDS             complement(15004..15279)
                     /gene="rpoZ"
                     /locus_tag="ACHKIK_002086"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO9083256.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(15334..15957)
                     /gene="gmk"
                     /locus_tag="ACHKIK_002087"
     CDS             complement(15334..15957)
                     /gene="gmk"
                     /locus_tag="ACHKIK_002087"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO9083257.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            16215..17897
                     /gene="ligB"
                     /locus_tag="ACHKIK_002088"
     CDS             16215..17897
                     /gene="ligB"
                     /locus_tag="ACHKIK_002088"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO9083258.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(17894..18511)
                     /gene="yicG"
                     /locus_tag="ACHKIK_002089"
     CDS             complement(17894..18511)
                     /gene="yicG"
                     /locus_tag="ACHKIK_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO9083259.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(18803..19627)
                     /gene="dinD"
                     /locus_tag="ACHKIK_002090"
     CDS             complement(18803..19627)
                     /gene="dinD"
                     /locus_tag="ACHKIK_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO9083260.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(19848..20711)
                     /gene="yicC"
                     /locus_tag="ACHKIK_002091"
     CDS             complement(19848..20711)
                     /gene="yicC"
                     /locus_tag="ACHKIK_002091"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO9083261.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            20838..21554
                     /gene="rph"
                     /locus_tag="ACHKIK_002092"
     CDS             20838..21554
                     /gene="rph"
                     /locus_tag="ACHKIK_002092"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO9083262.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            21620..22261
                     /gene="pyrE"
                     /locus_tag="ACHKIK_002093"
     CDS             21620..22261
                     /gene="pyrE"
                     /locus_tag="ACHKIK_002093"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO9083263.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(22298..22894)
                     /gene="slmA"
                     /locus_tag="ACHKIK_002094"
     CDS             complement(22298..22894)
                     /gene="slmA"
                     /locus_tag="ACHKIK_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO9083264.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(23001..23459)
                     /gene="dut"
                     /locus_tag="ACHKIK_002095"
     CDS             complement(23001..23459)
                     /gene="dut"
                     /locus_tag="ACHKIK_002095"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO9083265.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(23437..24657)
                     /gene="coaBC"
                     /locus_tag="ACHKIK_002096"
     CDS             complement(23437..24657)
                     /gene="coaBC"
                     /locus_tag="ACHKIK_002096"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO9083266.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            24829..25497
                     /gene="radC"
                     /locus_tag="ACHKIK_002097"
     CDS             24829..25497
                     /gene="radC"
                     /locus_tag="ACHKIK_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO9083267.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            25714..25950
                     /gene="rpmB"
                     /locus_tag="ACHKIK_002098"
     CDS             25714..25950
                     /gene="rpmB"
                     /locus_tag="ACHKIK_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO9083268.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            25971..26138
                     /gene="rpmG"
                     /locus_tag="ACHKIK_002099"
     CDS             25971..26138
                     /gene="rpmG"
                     /locus_tag="ACHKIK_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO9083269.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            26236..27045
                     /gene="mutM"
                     /locus_tag="ACHKIK_002100"
     CDS             26236..27045
                     /gene="mutM"
                     /locus_tag="ACHKIK_002100"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO9083270.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(27084..27563)
                     /gene="coaD"
                     /locus_tag="ACHKIK_002101"
     CDS             complement(27084..27563)
                     /gene="coaD"
                     /locus_tag="ACHKIK_002101"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO9083271.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(27571..28848)
                     /gene="waaA"
                     /locus_tag="ACHKIK_002102"
     CDS             complement(27571..28848)
                     /gene="waaA"
                     /locus_tag="ACHKIK_002102"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO9083272.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            29261..30319
                     /gene="rfaQ"
                     /locus_tag="ACHKIK_002103"
     CDS             29261..30319
                     /gene="rfaQ"
                     /locus_tag="ACHKIK_002103"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO9083273.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            30316..31440
                     /gene="waaG"
                     /locus_tag="ACHKIK_002104"
     CDS             30316..31440
                     /gene="waaG"
                     /locus_tag="ACHKIK_002104"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO9083274.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVCSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAPYIVEANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARYYADTQDLYSLPEKAADIITGGLDG"
     gene            31433..32230
                     /gene="rfaP"
                     /locus_tag="ACHKIK_002105"
     CDS             31433..32230
                     /gene="rfaP"
                     /locus_tag="ACHKIK_002105"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO9083275.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMHGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         7663 a         8033 c         8684 g         8006 t
ORIGIN      
        1 aatgaacaaa ttaatcttga tggcagtctg attattggtg aagtttatag tcagtttttt
       61 tcgccagcgg ttaaagaaat agaatagtcg tataaaaaag ggtgttctgg ggcgagtaaa
      121 acaacatctt caccttaatg gcgatacagt tggctgagag ccagcagggg gcaaatcacc
      181 tgcaaataaa cacggcacat gctggcgcgc atatcgcgga tgcgttcgag gacagaagaa
      241 ccgacaggcg gattcttcag ccgctcatcg tccatcacaa aaccttagac caggtattcc
      301 tgaagccgat tgaatattga gcagtagaaa atatctggat tgacagtatt acaaggggtt
      361 ggagaacgtc agaagaggca ttttggcgga agatcacagg agtcgaacct gcccgggacc
      421 gctggcggcc ccaactggat ttgaagtcca gccgcctcac cggagacgac gatcttccgc
      481 gcctcgattg ctacatggag gcggggcgca ttatagctac ttccttgagt ttctacatcc
      541 cccagatcag ttttttccgc ctaatacacg cagcgtatta tcacgaaagc attatagttc
      601 atataaaacc tatattttac aatgatatat gagcgaaatt aacgctacca cgatcacata
      661 aaacaagaat cataaattaa taaccagata tcggaatatt cgctctcaca gggatggttt
      721 acaaaatgcg ttatcggtct cagcacccct atagcatcaa ggaaaagcag atgaagagtg
      781 aagtgttgtc cgttaaagag aaaattggtt atggcatggg agacgccgcc agccacatta
      841 ttttcgataa cgtaatgtta tatatgatgt tcttttatac cgatattttt ggcattcctg
      901 ccggttttgt cggaaccatg tttttggtcg ctcgtgcact ggatgcgatt tccgatcctt
      961 gcatggggtt gttggccgat cgaacgcgct ctcgctgggg taaatttcgt ccgtgggtac
     1021 tgtttggcgc actgccattc gggatcgtct gtgtactggc ctatagcacg ccagatctca
     1081 gtatgaacgg caaaatgatc tatgcagcaa ttacttacac cctacttacc ttactttata
     1141 ccgtcgtcaa tatcccttac tgcgcattgg gtggtgtaat caccaatgac ccgactcagc
     1201 gtatctcgct gcaatcctgg cgttttgtgc tggcgaccgc gggaggcatg ctttctactg
     1261 ttctgatgat gccactggtt aatttaattg gcggtgataa taaaccactc ggtttccagg
     1321 gcggtatcgc ggtcctttcc gtggtggcat tcatgatgct ggcattctgt ttcttcacca
     1381 ctaaagaacg cgttgaagca ccacctacaa caacgtctat gcgggaagat ttacgtgata
     1441 tctggcaaaa cgaccagtgg cggattgtcg gtttactaac cattttcaat atcctggcgg
     1501 tgtgcgtacg cggtggggcg atgatgtatt acgtcacatg gattttgggc acgccggaag
     1561 tgtttgtcgc ttttctcacc acttattgcg tgggtaacct gattggttcc gcactggcaa
     1621 aacctctgac cgactggaaa tgtaaagtca ctatcttctg gtggacgaac gccctgctgg
     1681 cagtgattag cctcgcgatg ttctttgttc ccatgcaggc cagcatcact atgtttgtct
     1741 tcatcttcgt gattggtgtg ttgcatcaac tggtgacacc tatccagtgg gtaatgatgt
     1801 ccgataccgt cgactacggc gagtggtgca atggtaaacg cctgaccggg atcagttttg
     1861 ctggcacgct gtttgtgctc aaactggggt tggccttcgg cggcgctctt atcggctgga
     1921 tgctggctta tggcggatat gatgcggcag aaaaagcgca gaacagcgcc acgattagca
     1981 tcattattgc gctattcacg attgttccgg cgatctgtta tttgctgagc gcgattatcg
     2041 ctaaacgcta ctactcactc acgacgcaca atctgaaaac cgttatggaa cagctggctc
     2101 agggtaaacg ccgttgccag caacaattca cctctcaaga agtgcagaac taaggaacgg
     2161 caatgaaaat tagcgatgga aactggttga ttcaacctgg cctcaatttg attcacccgc
     2221 ttcaggtatt cgaggttgaa cagcagggta atgaaatggt ggtctatgct gccccccgtg
     2281 atgtgcgtga acgtacctgg cagcttgata cgcctttatt tacgctgcgc tttttctccc
     2341 cacaggaagg tattgtcggt gtgcggattg agcattttca gggggcgctg aataacggtc
     2401 ctcattatcc gctcaatatt ttgcaggacg tgaaggtcac aatcgaaaac acagaacgtt
     2461 atgctgagtt taaaagtggc aacttaagcg cgcgtgtcag caaaggtgag ttctggtcac
     2521 tggattttct gcgcaacggc gaacgtatta ccggtagtca ggtgaaaaat aatggctacg
     2581 tgcaggacac gaataatcaa cgcaattata tgtttgagcg gcttgatctt ggcgttggcg
     2641 aaacagttta cggtctggga gagcgcttta ctgccctggt gcgcaatggc cagacggtag
     2701 agacctggaa ccgggacggc ggcacaagta ctgaacaggc gtataaaaat atcccgttct
     2761 acatgactaa ccgtggttat ggggtactgg tcaatcatcc ccagtgtgtc tcttttgaag
     2821 tgggatcgga gaaagtctcc aaagtgcagt tcagcgttga gagtgaatat ctcgaatact
     2881 ttgttatcga cggcccgacg ccgaaagcgg tacttgatcg ttatacccgc tttactggtc
     2941 gtccggcgct gccgcccgcg tggtccttcg gcctgtggct aaccacttca tttaccacca
     3001 actacgacga agcgacggta aacagcttta tcgatggtat ggcggaacgc aatctgccgc
     3061 tgcatgtttt ccactttgac tgtttctgga tgaaagcctt ccagtggtgc gattttgagt
     3121 gggacccgct gactttccct gacccggaag ggatgatccg ccgcctgaaa gcgaaagggc
     3181 tgaaaatctg cgtctggatt aacccctata tcggtcaaaa atcccccgtc tttaaagagt
     3241 tacaagagaa aggctattta ctcaaacgcc cggacggttc gctatggcag tgggataaat
     3301 ggcagccagg tctggcgatt tatgacttta ccaatccgga tgcctgcaaa tggtacgccg
     3361 acaaactgaa aggtctggtc gcgatgggcg ttgattgctt taagaccgac tttggcgaac
     3421 gtatcccaac tgatgttcag tggtttgacg gttccgatcc gcagaaaatg cataaccatt
     3481 atgcatacat ctacaacgaa ctggtgtgga acgtgctcaa ggacaccgtt ggtgaggaag
     3541 aagctgtctt gtttgcccgc tcggcctccg tcggtgcgca gaaattcccg gtacactggg
     3601 gtggcgattg ttacgctaac tacgaatcaa tggcggaaag cctgcgcggt ggtttgtcta
     3661 ttggcctttc aggttttggc ttctggagcc acgatatcgg cggctttgaa aataccgctc
     3721 cggcgcacgt ttacaaacgc tggtgcgcgt ttggtttgct ctccagccat agccgtttac
     3781 acggtagcaa atcttatcgt gtgccgtggg cctacgatga tgagtcctgt gatgtagtgc
     3841 gcttcttcac gcaactgaaa tgccgcatga tgccgtatct gtatcgtgaa gctgcgcgtg
     3901 cgaacgcgcg gggtacgccg atgatgcggg ccatgatgat ggagttcccg gacgatccgg
     3961 cttgtgatta ccttgaccgt caatacatgt taggcaacaa cgtgatggtt gcgccggtgt
     4021 tcactgaagc gggcgatgtg cagttctacc tgccggaagg tcgctggaca cacctgtggc
     4081 acaacgatga actcgacggt agtcgctggc ataaacagca gcacggcttc ctgagtctgc
     4141 ccgtttatgt gcgtgataac actctactgg cgctgggcaa caacgatcaa cgtcccgatt
     4201 acgtgtggca cgaaggcacg gcattccacc tctttaatct gcaagacggg catgaagccg
     4261 tctgtgaagt gcccgctgct gacggatcgg tgatctttac tttaaaagca gcacgtactg
     4321 gcaacacgat tactgtgact ggtgcgggcg aggcgaagaa ctggacactg tgcctgcgca
     4381 atgttgtgaa agtaaatggt ctgcaagacg gttcgcaggc tgaaagtgag caggggctgg
     4441 tggtgaagcc tcaagggaat gcgctgacaa ttacgttgta atctattgat ggaatgccgg
     4501 gtacgtctga gtcgtacccg gcgagattga tgtttactgc accagctctg ccgacgtaac
     4561 ttcaccgctg gaaacgatgc cagcattcat ggtttgagtc acttgctgct ttgcggcgtt
     4621 cacggcatgc aattgcccgc taaccgtagg tttcagcggg gcattggcct gaatatctcc
     4681 actggcggta agctgaatat tgccgtcgcc agtcaacggt aattcaggcc atccccattg
     4741 ttgcaaaata ttcaccggca caccgcgtcc attcaggctg atatgggtct gacgttgtgg
     4801 cgtttgtgaa acactggcag tggcttccag aatgcctttt tcagtaaatg cactcagttc
     4861 gctgatattc accgtgctgc tgttggcggt cagcgccagc gacgggcgac gaacatcaac
     4921 acgattgaat gtcgcggcgg cggcattcag attcgccgag ccactccaga cgccccattt
     4981 atgatcggta accagcgtca ggttggcacc gtaaccatcg agcgcggtga gctgccacgg
     5041 gaagtcaggg tcgatatcaa tgatcagatt gcggctggcg ctaaatctct tcagttgcag
     5101 gctgtttaac caaccgggtg tcgtttccat ccacaactgt tgccagtttt tcggcaaggt
     5161 atattccagc ccggcaattg ccacatcatc aaggatcaac gttttcccgt cacgcagcca
     5221 gttccctgac gttctgacca tacccccttc ccagcggctg gtgaactggc gcagcgctac
     5281 gccctgcggg gaaaattccg cgtttataat cgggtcaaat aaatgcagcg aaccataaat
     5341 gaactcgcta gcgttcatcg acagtttgcc ttcctgtgtc tgccagtcat ctttactgaa
     5401 ggtcatgttg cgcaagctga gatcgaggtc ggtcaccgcc cagtccggac cttgcaaacg
     5461 agcatcgatc acttccaggc gaccaatctg caacgacggg acagagcgta atggcgcaaa
     5521 gaagtcggtt agcgattttt cgctttgtag acgtatatcc gccatgcgca ggttttccac
     5581 ttgccagctg ccgtcggcgt tacgctgcgc gtttccggtt aatgtcccgc gggcgatgtc
     5641 ggcacccagg ttagtcagcg taacgcgatc gttatcaata ctgccttcaa tcagtacatt
     5701 ggtggcagga acatcattga gcgaaagcga tccggcacta aactgaatct gcgccttcgt
     5761 acccagcact ttaccggctt ttggtgacca cggaactacg ccgccattta tccgctgcgc
     5821 gctcagtttc cattcgctat tcgggctatt aaacgccata tcacgcagtt gcagacgatc
     5881 ggctttgaac ggtagcggcg cggtctggtc ggtgagattc agcgtcccgt tttccagcag
     5941 gatggtatcg acatggcgtg gttcggtcag ttgccgactg cttagcgcaa tgtcgacact
     6001 ttttgccacc agggtcgcgg gctggccatc acggccaaac gtgacgttct ccagcacgat
     6061 atgagatggc gcggaaaaac ggtgatccat cgccccgaag gccagatgat agtcgctatt
     6121 ctcggaaacc catgcgctga tatgttctgc tccccagcgg gtttgcagaa gaaaatagag
     6181 gccagcgatc accactaaca gagcgatgag aatgtagaga agcagcttcc caataaattt
     6241 catggtcttc catcccgtga aatgcacata agggagttat gcacgattta cgcgcaatcc
     6301 tcaaggcggg aatggtgaaa gaggtgagac tgcggcggta attatcttac cgccgccagt
     6361 gaattactgt ttttctggtg ggaaaatcag attcagcacg atggcagtaa taccgcccgc
     6421 ggcgatcccg gaggagagca ggtttttcag ccattcaggg gcaaactgca aaatcagcgg
     6481 ctgctgagac acgcccagac caaccgccag cgacagcgcg ataatcagaa tcgcccgacg
     6541 gttcagcggt tcacgagaaa cgatacgcac accggaggcg gcgatagtgc caaacattac
     6601 aagcgttgcg ccgcccagaa ccggttctgg aatgtgttgt acaaaaccgc tcactgccgg
     6661 gaacagaccc agcacgatca acatcagcgc gacgacaaaa ccgacatagc ggctggcaac
     6721 accagtcaac tggatcactc cgttgttttg cccgaagcag gagttcggga aggtgttaaa
     6781 caccgccgaa acaaacgagt tcaggccgtt tgccagcacg ccgcctttca ggcgtttcat
     6841 gtacagcgga ccggacactg gctgttcgga aacgtcagag gtcgccgtga tatcgccaat
     6901 ggtttccagc gaagtgatca taaagaccag catcagcggc agcagcagac tccattcaat
     6961 gccaagaccg taatagagcg gcgttggcac cataatcagt tcttgcgtca tcggttcgtt
     7021 gctttctggc aacatgccca taaaccacgc cagcgcatat ccggccgcca tcgcaatcac
     7081 cagtgaggcc acgcgtaagt aagggttacg ttgacggtta agcaggataa ttaaggctaa
     7141 gaccacgcct gccagcagca gatttttcgg tgcgccgaag gtgttatcgc tcatggctgc
     7201 gtaaccgccg ccaatggacg ttaacccaac ctgaattagc gacaggccga taatcatcac
     7261 cacaacgcca gaaaccagcg gcgtaataat gcggcgcgcc agatgcagaa cgcgggagat
     7321 caccatctcg gtgcaacttg ccagcatcaa cgtgccgaac aaagccgcca tcatggtagg
     7381 aacatcagca ccaccggttt tcagcgcggt accgcccata atcagcgggg caacaaagtt
     7441 gaagctggtg ccctgaatag acaacagccc ggagccaacc ggaccccagg ccttaatttg
     7501 aataatcgat gccacaccgg aggcaaacag cgacatacta ataatgtgtt gcgtgtcttg
     7561 tgccggtaaa cccagcgcct ggcagattaa tagcgctggc gtgatcaccg caacgaacat
     7621 cgccagcaga tgctgacagg cggcaaacag ggtttgagga agcggcggac gatcttcaag
     7681 acggtaaatc agttcgctgt tttgagtctg cgcaaccggt tgcgcatttt ctgactcgag
     7741 ggtggaaaca gacatcgaaa acaatcccgt ggtagcaaag cgggcatttt agctgaccga
     7801 aagagaaaag caaacgtttg ccacatcctc atctaaatat ttaaatgaga taaatgcact
     7861 tttttatata acttttggta tttttctgct taaaatccat gccatttgtt cattaacagc
     7921 tgagaatttg acccaggaaa gcataactcg atacccgtca tagttcatga agttgccact
     7981 gcgcgatgaa gtatgacgag tatgaaagag tgatgcggat acaaaggagt aactatgttt
     8041 catctcgata ctttagcaac gcttgttgcc gcaacgctga cgttgctgct cgggcgtaag
     8101 ttggtccatt ccgtctcctt tttgaagaaa tacaccatac cggaacctgt tgcgggtggt
     8161 ttgttggtgg cgctggcgct actagtactg aaaaaaagca tgggctggga agtcaacttt
     8221 gatatgtccc tgcgcgatcc gttaatgctg gctttcttcg ccaccattgg cctgaacgcc
     8281 aacattgcca gtttgcgtgc cggtgggcgt gtggttggca tcttcttgat tgtggttgtt
     8341 ggtctgttgg tgatgcaaaa tgccattggc attggtatgg ctagcctgtt agggcttgat
     8401 ccgctgatgg ggctgttggc cggttctatt acgctttccg gcggtcacgg tacgggcgct
     8461 gcgtggagta aattgttcat tgaacgttat ggcttcacca atgcgacgga agtggcgatg
     8521 gcctgtgcaa cgttcggtct ggtgctgggc ggcttgattg gcggtccggt ggcgcgctat
     8581 ctggtgaaac actccaccac gccgaacggt attccggatg accaggaagt cccgacggcg
     8641 tttgaaaagc cggatgtggg acgcatgatc acctcgctgg tgctgattga aactatcgcg
     8701 ctgattgcta tctgcctgac ggtggggaaa attgttgcgc aacttttggc tggcactgct
     8761 tttgaactgc cgaccttcgt ctgtgtactg tttgttggcg tgattctgag caacggtctg
     8821 tcaatgatgg gcttttaccg cgtctttgag cgtgcggtat ccgtgctggg taacgtaagc
     8881 ctgtcgttgt tcctggcgat ggcgctgatg gggctgaaac tgtgggagct ggcttcgctg
     8941 gcgctgccga tgctggcgat tctggtggta cagaccatct tcatggcgtt gtatgccatc
     9001 ttcgttacct ggcgcatgat gggcaaaaac tacgatgcgg cagtgctggc tgcgggtcac
     9061 tgtggttttg gcctcggtgc aacgccaacg gcaatcgcca acatgcaggc gatcactgaa
     9121 cgctttggcc cgtcgcacat ggcgtttttg gtggtgccga tggtcggtgc gttctttatc
     9181 gatatcgtca atgcgctggt gattaagctg tatttaatgt tgccgatttt tgccggttaa
     9241 aaatgaaaaa gcgtcggaag gaaccggaaa cgctacgtga acactgtcgc catatttttg
     9301 gtgatgagcc gccagtgctc tgtgtatggg aaacggaatt tgattacgca gatgccgaac
     9361 tcaaggcgct ggcggctaaa gagtggcagc agatcagcga atgggatttg agtgcttact
     9421 atgtactcaa tctcgtttat aacgaaccga tgcaaataga gttgtttcgc tatctgtttc
     9481 cgctctgcct ggcgcaatgg catgaaaccg ttttagctgg cggttatggt gatcacttcg
     9541 aagaatcctt aatgaaggca ttgtgtcggc cctatctctg gcaggagatg atgaatgctt
     9601 cccaacgcca gcaggtacgg caatttttac tggacaccgc gttgcagcgt atggataacg
     9661 agcgtggttt caataacgtt ctttgctggc tggcggtgtt caatactctg ggtggggccg
     9721 cgccgttaat tcgttctctc tggtcgcgct ggtgggcgct ggatacgcca ggaaaagcgg
     9781 tgtgtgctat tcagtatgcc gcgcatctaa tttatcccat agaagccaat ccgttatggt
     9841 cgcaagaatg gataggctgg gggcatccat taggccataa agatggctgg tcctctgata
     9901 atcgcgcttt tcttcgccaa atgttaacac ctgaaatgat tgtggctggc gtacaggcag
     9961 cggctgaaat tctgcgtggt gaaccggaag gcgcaatggc ggcgcgaatt gcacaggatg
    10021 cgtatgaggc aatggatatc ctcactatcc agattgaaga tttactgcgt gacttgtctt
    10081 gtgatgagtc tggtcatgcg ttggagtaac gttccgtctc cggcatccag cgttctatca
    10141 gggcttttgc ttgttgtggg taacgttcgt gaatatgtcg cgccaggcgc tgaacttccg
    10201 ggatcatcgc ctgatcgcgc agtaaatccg ccactttaaa ctcagcatta cccgtctgac
    10261 gcgtacctaa cagttcaccc ggtccgcgaa tctctaagtc tttctgtgca atcacaaaac
    10321 cgtcgttgct gtcgcgcagc acttgcagcc gaatttgcgc ggttttggag agcggcgttt
    10381 tgtagagcag cacgcagtga gaagccaccg caccacgacc tacacgcccg cgaagctggt
    10441 gtaactgcgc cagacccaga cgctccgggt tttcgataat catcagactg gcgttaggca
    10501 catcaacgcc gacttcaata acggttgtgg caaccagcag gtgtagctca ccttgtttaa
    10561 acgacgccat caccgcctgt ttctcggcag gtttcatccg cccgtgtacc aggccaacgt
    10621 tcaactctgg tagcgccagt ttcaactctt cccaggtagc ttccgccgcc tgcgcttcca
    10681 gcaattccga ctcttcaatc aacgtacaaa cccagtatgc ctgacgacct tcagttatgc
    10741 aggcgtggtg cacgcggtca atgatgtcgg tacggcgggt atcaggaata gcgaccgtag
    10801 tcactggcgt gcggcctggc ggcagctcat ctatcaccga ggtatcgaga tcggcatacg
    10861 cagtcattgc cagcgtgcgg gggatcggcg tggcggtcat gatcaactga tgcggatgga
    10921 agccctgctg ctggcctttc tcccacaatg ccagacgctg atgcacgcca aaacgatgct
    10981 gttcgtcgat aatcaccagc gccaggccgt taaactgcac ctgttcctgg aagatggcgt
    11041 gtgtaccgac aatcatctgc acctgaccgc tggcgatggc ttcctgctgt gccagccgtg
    11101 ctttaccttt ctgcttaccg gcgagccagc ccacttcgat accgagcggt gcaaaccagt
    11161 tgcggaagtt attggcgtgc tgctcggcga gtaattcggt tggtgccatc aatgctacct
    11221 gtttgccgtg ggcaatcgca cgcaacgcgg cgagggcggc gaccagcgtt ttaccggaac
    11281 ctacatcgcc ctgcaccaga cgcatcatcg gcacatccag cgccatatcg cgctcgatct
    11341 ccgccactac gcgtgcctgt gcgcccgttg gcttgaacgg taaggcggcg aggagtttat
    11401 ttttcagcgt gtcattggcg ctcagcggct gggcatgaaa acgctgtgct ccggcacgta
    11461 aggctaacat gctgaggttg tgcgccagca gttcttccag aatcagacga cgttgcgccg
    11521 gatgctgccc ggtttccaga tcgctaagct gtagcgtcgg tggcgggcgg tgcaaagtgc
    11581 gcaacgcttc cggtagcgtc atcattcctt gtgacagttc cggcggcagg agttcttcaa
    11641 tggcgcaggt gtcgagcaga tccagcgcct ggtcggttaa tttacgcagc gtggcctgct
    11701 ttacgccttc cgttgttgga taaaccggcg tgagcgtttc ctgtaattct ggcgtgctga
    11761 gatcgccctg cacgcggtat tccgggtgga tcatctccgc accatattta ccgcgctttg
    11821 cttcgccata agccagtaca cggcggcccg ccgccaggct atttttcatt gccgcgctga
    11881 aattgaaaaa gcgcatggtg aggatgccgg aaccgtcgct gatctggcag gtcatcatcc
    11941 gccgaccgcc gaaggagata ttgcagttca gcacttcgcc ttccaccgtg gcataaacgc
    12001 ccggcagtag ttctccgatg gggtagagat gggtgcgatc ttcgtagcgc aggggaaggt
    12061 gtaagagtag atcctgcacg gtatgcaggt tgattttcgc cagtttgtta ctaagtgctg
    12121 cgccaacgcc cgttagggaa ctgagtggga cagcatctaa caggcgacct ttcatggcac
    12181 ttaccctgca gcctgcatag tagcccacca gtcggcatca gcttcgatct cgccttgctg
    12241 attgacgtgg ggataaggca ggcctttgcg ttttgcgact ttcgccagca ccggatagcc
    12301 gccttcaaac aacaggcgtt gttgctctgc ttccggcaac atgctgtttt cacgcaggta
    12361 catgcctgca ttttgccgct gacgctgggc ttcgtaaaga atgagggctg aggcaacgga
    12421 cacattcagc gactgcacca tgccgatcat cggaatgatg atgtcctgat ccgccagggc
    12481 caatgcttcc tgcgtgatgc ccgttttctc ctgtcccatc aaaatgcagg tcgggcgagt
    12541 gtaatcaatt tcgcggaaat cgacagcgtt atcagaaaga tgggttgcca gaatctgcat
    12601 gccctggcct ttgagatgag cgacggcatc gccaatggtg cggtgtgttt tcacctgtac
    12661 ccagctgtta ctacccgccg ctgccgaagc catggtgcgc atgcggctac caggccagac
    12721 ggcgtgaact tcatgtacgc caacggcatc tgcggtacga ataatcgcag aaacgttatg
    12781 aggtttgtgg acctgctcca tgcagacggt cagatcaggc tgccgcctgg cgagcatttc
    12841 gcagatgcgt gcataacgtg ttgggttcat aaaacattaa tttcggtttc gggtgacttt
    12901 aatcacgtct ggcatcacgc ggattttgcg catgatattc gccagatgca cacggtcacg
    12961 agcggtcaga cgaataaagg cgctgtagac gcgaccatct ttctcttccg tattcaaact
    13021 ttgaatattc gaggtcgtgg tgttaattgc cgccgtcagg tttgccagcg caccctgatg
    13081 attgaacatc tccaccttga tttcggtgat gaactcctgc gccgtctctt tatcccattc
    13141 cacagccata aacttctctg gctctttctg gtagccacgg atgttacggc aggattcatg
    13201 gtggatcacc agacctttac cggggctgac gtgggcgata atcgggtcgc caggaatggg
    13261 gcggcagcat ttcgcaaagg tgatcagcac gccatcagcg cctttgatcg gcagatgccc
    13321 gtggctttga gtcgccggtg gaatggaggc gtccccatgt tgtagatttt tcgcgaccac
    13381 cacgctcatt gcgttaccaa ggccgatttc tgccagcaga tcgtcaagcg ttgccagctt
    13441 catgcgatcc agctcgcgct gaatattttc ctgcgggatt tcattgagct tacggctacc
    13501 acccaaagca tggttgagca gacgacggcc caggcttaca gaatcatcac gcttgaggtt
    13561 tttcagcaac tgacgaattt tggcgcgcgc tttcgagcta acgacaaagt tcagccaagc
    13621 ggcattcggg cgagcgcccg gagcggtaat gatttcaacg gtctgaccgc tagtaagcgg
    13681 ctgcgacagc gggtaaggct ggcggtcgac gcgtgcgccc acgcaggcat ggccgatatc
    13741 ggtatgcact gcataagcga agtcgacggg cgttgcaccg gcaggcagct cgacaatgcg
    13801 cccttccggt gtgaaaacgt aaatctcatc cgggaagaga tcggatttaa cgctctcgat
    13861 aaattcaaac gaactaccgg cgctctgttg cagctccagc aggctttgca tccagcgctg
    13921 ggcgcggatt tgcgcggtgg tgctggtttc gccgtgctct ttataagccc agtgcgcggc
    13981 aacacccatc tccgccatct gatccatatc ttcggtacgg atctggacct caaccggcac
    14041 accgtgcggg ccgatcatcg aggtgtgcaa agactgatag ccgttcgctt ttggaatggc
    14101 gatgtagtct ttcacgcgac ccggacgcgg cttgtacagg ctatgcattt gcccgagcac
    14161 gcgataacag gtgtcggaat cattgacgat cacgcggaaa gcgtagatgt ccatgattga
    14221 gtgaaaacgc tgctctttga gcaccatttt gcagtaaatc gaataaagat gcttctcgcg
    14281 accactgacg cggcacggaa ttcccgcttc ctgcaaacgc ccttcgattt cagagaggat
    14341 tttttggatc atctctttac ggttgccgcg cgcggctttc accacttctt taattacgcg
    14401 gtaacgattg ggatacagcg cctcaaaacc cagctcttcg agttcggttt taatgtggtg
    14461 gatacctaaa cggtgcgcca gcgggctgta aatttcgaga gtttcacggg cgatgcggcg
    14521 acgtttgtcc gggcgaagtg agcccagcgt gcgcatgttg tgggtacggt cggcaagttt
    14581 gatgaggatg acgcggatat cctgcaccat tgccataatc atcttgcgaa agttttcggc
    14641 ctgcgcctct ttcttatcac ggaacttgag tttatcaagt ttcgacaccc cctctaccag
    14701 ctcggcgacg cttttaccaa aaagctgttc catatcctgg taggtggcgg gagtatcttc
    14761 aatcacgtca tgcagcagcg ccgccatcag cgtttcatag tcgagtttca tctcggccag
    14821 aatgcaggca accgctaccg ggtgcgtgat atagggttca ccgcttgaac gtgtttgccc
    14881 ctcgtgagca tcacgtgcaa cgagatacgc ctgccgcaga cgcttgattt ggtcttccgg
    14941 caggtaggtt tgaatcagtt gattcaggct ttcaaacaga tacaagggcg acccgctttg
    15001 tgattaacga cgaccttcag caatagcggt aacggcttgt aattcagcgg cttcctgctc
    15061 ttgctgttcc tggcgttcgc gaacgtcgag gatctggttg ttgatcagac cttcttcgat
    15121 ttcgcgcagc gcgattacag tggttttatc gttttcttcc ggtaccagcg gatcctttcc
    15181 gcctacctgc atctgacgag cgcgacgcgc ggcgaccagt accaggtcaa aacggttacc
    15241 aattttctct acagcgtcct gaacagttac gcgtgccata cttaaaaagc tccacaggtg
    15301 aagaaatgac tgggcatgat actgaaatca ggttcagtct gccaacaatt tgctgattaa
    15361 agcgtcatga cgctgctttt ggcggctcat gcgcagacgt tcggcgcgaa taatggtctt
    15421 caaatcggtc aacgcggtat cgaagtcatc attcacaatc agataatcat attcggcgta
    15481 atggctcatt tctgcaacag cttgcgccat acgctttgca atgacctctt cgctgtcctg
    15541 accgcgaccg cgtagacggc ggtccagttc aattttggac ggcggtaaaa taaagatact
    15601 ccgcgcgtgc ggcatcttct ggcgaatttg ctgcgcgccc tgccagtcga tatcgagaaa
    15661 aacatcgaca ccggtcgcca gtacttgctc aatggcctca cgcgaagtgc catagtaatt
    15721 accaaaaact tctgcgtgtt cgaggaacgc atctctgcta atcatttctt taaattcatc
    15781 atgattaaca aagaaataat gttcaccgtg gacttcacca ggacgcggtt ggcgtgtggt
    15841 gtgtgaaaca gaaacctggg tgtcatacaa cggttgggtt tttaataaag cctgaatcag
    15901 gctggattta cccgcgccac tgggggcaga aacaatataa agcgtgcctt gagccatgag
    15961 tatctttcgt atgtgattag cgaaataaag cctacatacg agcttattat acacggcgcg
    16021 gccatgtgac gtagcccttg ctcacacttt ttgcgcaaag ttgttgcgtt ttgcgtagct
    16081 gtttccgcat ttgcctgtga tttactgcaa taaagacgat tctcaggaag cgtcctcgca
    16141 gactccgttt ttgccactcg gctttgcctt caccacgttg tatatctgcc agcctgacta
    16201 cagcggaggt gaagatgaaa gtatggatgg cgatattaat aagtatcttg tgctggcaat
    16261 catctgtgtg ggcggtctgt ccggcctggt cgccagccag agcacaggaa gaaatttccc
    16321 gcctgcaaca gcaaataaaa cagtgggacg atgactactg gaaggaagga aaaagtgagg
    16381 tggaagacgg tgtttacgat cagttaagcg cccgtcttac gcagtggcaa cgctgctttg
    16441 tgagcgagcc ccgcgacgtg atgatgccgc ctttaaatgg tgcggttatg catccggttg
    16501 cccatacggg tgtacgcaaa atggcggata aaaacgcatt aagtctgtgg atgcgagaac
    16561 gtagcgatct ttgggtgcag ccaaaagttg atggcgttgc ggtaaccctg gtttatcggg
    16621 acgggaaact gaacaaagca atcagtcgcg gtaatggcct gaaaggcgag gactggacgc
    16681 agaaagttag cttaatttcc gctgtgccgc aaaccgttag cgggccttta gccaacagta
    16741 cgcttcaggg ggaaatattt ctccagcgcg aggggcatat ccaacaacaa atggggggaa
    16801 taaatgcccg cgcaaaagtt gctggcctga tgatgcgcca ggacgatagc gacacgctga
    16861 attctctggg cgtttttgtc tgggcatggc cggatggacc gcagttaatg accgatcgtt
    16921 taaaggagct ggctaccgca ggttttactc taacgcagag gtatacccgt gcggttaaaa
    16981 atgctgatga ggttgcgcgc gtacgcaatg agtggtggaa agcgaaatta cccttcgtca
    17041 ccgatggcgt ggttgtgcga ggggcgaaag aacccgaatc ccgccattgg ctaccaggcc
    17101 aggcagaatg gctggtggcc tggaaatatc aacctgtagc tcaggttgcc gaagtgaagg
    17161 cgattcagtt tgcggtgggt aagagcggta aaatatcggt ggttgcgtca ctcgcacctg
    17221 tcatgctgga tgataaaaaa gtccagcggg tgaatattgg ttccgtcagg cgctggcagg
    17281 agtgggatat tgcgcctggt gatcagatcc tcgtcagcct tgccggtcag ggtattcctc
    17341 gcattgatga tgtggtctgg cgcggtgcag aacgtacaaa accgacacca ccagaaaacc
    17401 gctttaactc gttgacctgc tactttgctt ctgatgtttg tcaggaacag ttcatttcac
    17461 gcttagtctg gctggggtca aaacaggttc tggggctgga tggtattggt gaggccggct
    17521 ggcgcgcgct gcatcagact catcgctttg agcatatctt ttcctggctt ttattaacgc
    17581 cagagcaatt acagaacacg ccggggatcg cgaaaagtaa aagtgcgcag ctatggcatc
    17641 ggtttaatct ggctcgtaag cagcctttta ctcgctgggt gatggcaatg ggaataccgc
    17701 taacccgggc ggcgcttaat gccagtgatg aacggtcctg gtcgcaactt ttatttagca
    17761 cggagcagtt ctggcagcag ctgccgggga ctggatcggg gcgcgccaga caggttattg
    17821 aatggaagga aaatgcgcaa atcaagaagc tgggcagttg gctggctgcc cagcagatca
    17881 caggttttga accttagtgt ccttcgtggc tgtagtaaaa gaccggtaat cccagcttaa
    17941 ggcgtagcgc cagtaagcgg gcaaaaaagc cgaataccag ggtggagata attaccacat
    18001 catggttaga aacatagtgt tgcagcgcga tgtacagcac ggcggaggca aatgagacac
    18061 cggcatacaa ctctttctgg aataccagtg ggatacgttt acagaacata tcgcgcaaaa
    18121 cgccgccaaa cacgccggtg gttaccgccg cgacaacggc aataattggg ccgtgcccca
    18181 tatccagtgc aacctgtgcg ccgatgatag aaaagaccac cagtccaagc gcatcgagca
    18241 ccagaaacac tttgcgcagg taaggcatta caggagcaac gatagtggtg agtactgcgg
    18301 cggtagcgac gataatcaca tattccgggt gtttgaccca gccgagcgga tagtggccca
    18361 gcagaatatc gcgcactgac cctccgccaa ttgcggtggc ggtcgcaata ataattacgc
    18421 caaatgtatc catgcggcgt cgcccggccg ccagagcccc cgtcatggct tcggcagtaa
    18481 tgccaaccaa atacaaaatg tgtaacagca tggttctctc cctaaaaatc tgtctggcag
    18541 ggtagcgatt tgtgcgtatt gtcacgattg agatttttta aggtgagagg aaattgataa
    18601 cttgtagtta tgtgaaatcg ctattttctg tagcgtagat gcattcttct gacttcttgt
    18661 ctgagaaaat gcataagaaa aaataggtta aaattgaggg gtggaaaaga aaggtgcaat
    18721 gtttagccat tgtgttcacc gcacctggac tttggcttac gcatcgggta tctcaatcaa
    18781 caaaaagagt ttttagtttc tgctatttct tctctgtaat ttttacactg ttttccaact
    18841 gcttgatgct gacctgcggg gtcggcaact cctcaggcat ggttccgcca agttcctgaa
    18901 tggtttgcct caccttgcta cccacgtcaa agtgtgtggt attagcttgc tgttttgaat
    18961 tcacgccatc ccgcttgagt ttttcttctg tttgggtagc tcgaaagaga ttagccgcca
    19021 gttctgttga acccatatga tccaggatct tctgactctt tttcagcccc ttccgctgat
    19081 ggatggcttt ctgatctaat ccaccataca gcccccggta accatgattc tgaaatatgg
    19141 cgaaatcgat agctgtcgct accccagctt gctgcgctgc ctcaaccaat tgtttgttat
    19201 gttctttcaa ttcatttctc agaaaaagac gcttttcgtc ttcacgaagt tgcctgaaag
    19261 cttcatcgtc agccagctcc tgccgtcggg tctggatagc aaaataagtt tgccctgccg
    19321 caataaccgg tttcgcaggg tcgccgtttt gtactaccag gtaacaggca tagcgggaga
    19381 gatgaacatc ttcaagttct cgttgagccc ctgaacctaa tacaaccatt ttggtggttt
    19441 caacgaaatg gtcagatgca gcttgattgc tggcttcgca cgcttgagtc gcgcgggcca
    19501 gtaccttttg aaaattgcgc cagtcccgat aatccagtaa cggtgccaat tctctagctg
    19561 accaaaattc ttgcccttca gtgccataat gccgtatttc ttcaaaaggt tgatgatgtt
    19621 cgttcattgc ccactccatg tgatatcgat tgcaaagaaa gtaaatctgt aactgtattt
    19681 atatacagtt gtgagtatct cacgttctca gtgtgctggg caaaaaaaca tcgcaaaatc
    19741 tggagcaggc acgcaaaaca gaattgaagt tgcggggaag aaaaatgccg ctctgtttga
    19801 gtgggcggca tttgtgaagg ctgaaaagta agaattagtg ttacgagtta ctcaatattc
    19861 tgaatctgct cgcgcatctg ctcaatcaac actttcagct caatcgcgga gtttgtcact
    19921 tcggcattga tagacttcga cgcaagagtg ttcgactcgc ggttgaattc ctgcatcata
    19981 aagtccagac gacgaccaac ggcttctttt ttcttcagaa tgttgtaggt ctctttgaca
    20041 tgcgcttcca ggcggtccag ttcttcggca acgtcaattc gttgtgccag cagaaccaac
    20101 tcctgctcca ggcggttgtt ttccagttgc acttgagcat cttccagctt cgcgaccaga
    20161 cgctcacgct gccattgcag gatttccggc atatgggcgc ggactttgac cacttcggcg
    20221 gtgacgcctt ccagacgctg ctcgatcaat gctttcagcg cctgaccttc ggtttcacgt
    20281 gcgacgataa agtcgtccag ggtaccatcc aacgccgcga gaatttcagc ggcaatcgcg
    20341 tcgagatcct gctcctgggc tgccattacg cccggccagc gcagaatatc aaccgggttg
    20401 atttcccctt cgtcactctg catttttacc cagttcgcgg cagttaccag ctgtttagcc
    20461 agtttttcgt tgaggatcag ctcaccttgc gcgctaacat ctggctcata gcgcagggta
    20521 cattccactt taccgcgcgt caggcgagaa cgaatacgct cgcgaacgac aggttcaagg
    20581 ctacggaact gctccggcag acgaaagtaa gtttccagat aacgctggtt taccgagcgc
    20641 atttcccagg ttgcgctccc ccattcaccc ttgatttcac gccgggcgta ggcggtcata
    20701 ctgcggatca tagacgttcc tgtttataaa aggagaggtg gaaggattat agccatcgat
    20761 gccttgtcag gataggaata accgccggaa gtccgtataa tgcgcagcca catttgtttc
    20821 aagccggaga tttcaatatg cgtccagcag gccgtagcaa taatcaggtg cgtcccgtta
    20881 ccctgactcg taactataca aaacatgcag aaggctcggt gctggtcgaa tttggcgata
    20941 ccaaagtgtt gtgtaccgcc tctattgaag aaggcgtgcc gcgcttcctg aaaggtcagg
    21001 gccagggctg gatcaccgca gagtacggca tgctgccacg ttctacccac acccgtaacg
    21061 ctcgtgaagc ggcgaaaggt aagcagggtg gacgcacaat ggaaatccag cgtctgatcg
    21121 cccgtgctct tcgcgcggca gtagatttga aagcgctggg tgagttcacc attacgctgg
    21181 actgcgacgt gcttcaggct gatggtggca cgcgtaccgc gtcgattacg ggtgcctgcg
    21241 tggcgctggc agatgcgcta cagaagctgg tggaaaacgg caagctgaaa accaacccga
    21301 tgaaagggat ggtagccgca gtttctgtcg gtattgtaaa cggtgaagca atttgcgatc
    21361 tggaatacgt tgaagactca gccgcagaga ccgacatgaa cgtagtgatg accgaagacg
    21421 ggcgcatcat tgaagtgcag gggacggcag aaggcgagcc gttcacccat gaagagctac
    21481 tcaccttgtt ggcgctggcc cgagggggaa tcgaatccat tgtagcgacg cagaaggcgg
    21541 cgctggcaaa ctgattttta aggcgactga tgagtcgcct tttttttgtc tgtagaaaag
    21601 taagatgagg agcgaaggca tgaaaccata tcagcgccag tttattgaat ttgcgcttgg
    21661 caagcaggtg ttaaagtttg gcgagtttac gttgaagtcc gggcgcaaaa gcccctattt
    21721 cttcaacgcc gggctgttta ataccgggcg cgatctggca ctgttaggcc gtttttacgc
    21781 tgaagcgttg gtggattccg gccttgagtt cgatctgctg tttggccctg cttacaaagg
    21841 gatcccgatt gccaccacta ccgccgtggc gctggcggag catcatgacc ttgacctgcc
    21901 gtactgcttt aaccgcaaag aggcaaaaga ccacggtgaa ggcggcaatc tggttggtag
    21961 cgcgttacaa ggacgcgtaa tgctggtaga tgatgtgatc accgccggaa cggcgattcg
    22021 cgaatcgatg gagattattc aggctaatgg cgcgacgctt gctggcgtgt tgatttcgct
    22081 tgatcgtcag gaacgggggc gcggcgagat ttcggcgatt caggaagttg agcgtgatta
    22141 caactgcaaa gtgatctcta tcatcaccct gaaagacctg attgcttacc tggaagagaa
    22201 gccggaaatg gcggaacatc tggcggcggt taaggcctat cgcgaagagt ttggcgttta
    22261 aagaaactcg ccggatgaaa agtcatccgg cgtcatatta ctgcaactgt gccgcaatta
    22321 gcggccagcg ggcgtcaaaa tcatccgtcg ggcggtattt aaattcgctg cggacaaaac
    22381 gtgacagcat accttcacag aaggccagga tctggcttgc cagcagggtt tcatcggtgg
    22441 tgtaaccttc accctcacgc attctctttt cacgcaatac ctggcgcagc tgcgcttcaa
    22501 tacgctcgaa cagctggttg atgcgccctt gcaggcgatc ctgttcaaac attagcgcat
    22561 gaccagtgag gatgcgggtc aggccaggat tacgctcacc aaaaccgaga agcagcaaca
    22621 caatcagacg caggcgcgct gtggtgtctt tctcatcttt cagaatcagg ttgatgcgag
    22681 taatcaggct atcttcgata aactcaatca ggctatcgaa catgcgggtc ttactgggga
    22741 agtggcgata cagtgccgct tcggaaacgc cgacagaggc ggccagtttt gccgtcgtga
    22801 tacgttggct tccatcgctg gattccagca tcagcgccag agactgaagt atttcctcgc
    22861 gacggttcct tttcgcagtt tgtttttctg ccatgttaca aaatacccct gaaaataagc
    22921 acttgccagg cggcacccac gctatgaccg caaacgaaat gtttgcggct atgttatgac
    22981 gttattcgga tgcgtatgtg ttactgacga ccagagtgac caaagccgcc ttcaccgcgg
    23041 tcggtggcgt cgaaatcttc caccagatta aattcagcct gtactaccgg aacaaaaatc
    23101 atctgggcga tgcgttcgcc aggttgaatg gtgaagctgt cctgaccacg gttccacacg
    23161 gaaatcatca actggccctg atagtcagaa tcgatcaatc ctaccaggtt accaagcacg
    23221 ataccgtgct tatgtcccaa tccggagcgc ggcagcatca ttgccgccag tgaaggatcg
    23281 gcaatatgaa tcgccagccc ggtcggaacc agcgtagtgt cacccggagc cagttctacg
    23341 gcgtcgtcga gacaggcacg caggtcaagt ccggcagagc cagaggtggc ataagtcggg
    23401 agcggaaatt ccttcccaac gcgcgggtcc agaatcttaa cgtcgatttt tttcatcata
    23461 acgggtcacg atctcgtcga gtaataattg gccaaggagc tctttgcgct caagcggtaa
    23521 gactttatct ccgtcctgcc agaaaaggtg taatgcgttg ttgtcgctgt taaatccttg
    23581 agttggctgg gaaacatcgt tcgcgcagat cagatcaagg tttttacgga tacgtttttg
    23641 ccgggcgtat tcttccacat tatttgtttc ggcggcaaat ccaacgacgt agggtcgatg
    23701 gtcttttagt gcggcaacgc ctgcgacgat atcggggttt ttaaccattt ttattgttaa
    23761 ttcatcaccc tgcgtggcct gctttttgat tttctctggg gccacggtag ctgcgcgata
    23821 atccgccacg gcggcgcagc cgataaaaat attttgctgc tgtacagaag cattcacggc
    23881 ggcttccatt tccagcgcag tcatcacatc aacacgttta acaaacggtg gcgtcggtag
    23941 tgaaaccgga cctgatacca gcgtgacgtt cgcgccacga cgggcagcgg cggcggcgat
    24001 agcaaaaccc atcttgccgg agctgtgatt agagatataa cgcaccggat cgagcggttc
    24061 acgcgtcgga ccggcggtaa tcataatgtt cagatgtttc aggtcgttga cgggcgaaaa
    24121 atgcgccacc gccatatcca caatggttaa cggatcgagc attcgcccag gaccgatatc
    24181 accacaagcc tgactgccac tgtctggccc ccagatgagc aaaccacggg aagcaagcac
    24241 ctctaaatta tgctgcgtgg cagcggcacg gtacatctgc tggttcatgg cggggagcac
    24301 ggctacaggc gcaggtgtag ccagacaaat cgtcgatacc aggtcattcg ccattccggc
    24361 agcaacacgg gcaatcaaat ctgccgtggc aggggcgaga atcactaaat cagcccattt
    24421 acccagctca atatggccca tagcggcttc ggctgccggg tccagcaaac tgtcggaaac
    24481 gggataacca gaaaccgcct gcaagctaag tggggtgata aaggcttttg ccgcttcggt
    24541 catggctacg cggacgtcgg ccccgcgatc gcgcaaacga cgcaccagtt caggggtttt
    24601 ataggcagca ataccgccgc taacgccgag aacgattttt ttaccggcca ggctcatgat
    24661 gatttttcct gttgggtgac accagaagtt ggcgatttta tcacaatact tttgttgtcg
    24721 tgccttcacc tgagaatcac tttgcgaggc gctttccagg attgaaaact ggccgtcgat
    24781 ttaacggaac ggctatgaca ggatgcgagc accacaaagg aggtgaaggt gaaaaacaat
    24841 gcacagctgt tgatgccgcg cgaaaaaatg ctgaagtttg gtattagcgc cttaacggat
    24901 gtcgagctgc tggcgttatt tctgcgtacc ggaacgcgcg gtaaagatgt attaaccctg
    24961 gcaaaagaga tgctggagaa tttcggctct ctttatggct tgttaacctc tgaatatgaa
    25021 caatttagtg gcgttcatgg aattggcgta gcgaaatttg cccagttaaa ggggattgct
    25081 gaactggcgc ggcgttacta caacgtgcgg atgcgtgaag aaagcccttt actcagcccg
    25141 gagatgacgc gggaattttt acaaagccag ctcacgggtg aggagcggga gatctttatg
    25201 gtgatctttc tcgactcaca acaccgggtt ataacgcata gccgtctttt ttccggcacg
    25261 ctaaaccatg ttgaagtcca tcctcgggaa attatccgcg aagcgataaa aataaacgcc
    25321 tcggcgctga tccttgcaca taatcaccct tcgggttgtg ctgaacccag taaagcggat
    25381 aaactcatta ctgaacggat aataaagagt tgtcagttca tggatttacg cgtgctcgac
    25441 catatcgtga ttgggcgtgg agagtatgtt tcttttgccg aacgcggctg gatttaaccc
    25501 gctatgcgcg atccttcggg atctttgtct gttcgggact tgagcacatc gctgagtcag
    25561 cgtatactac gccacctttg agaatctcgg gtttggcatt tgggcctggc aatcgagagt
    25621 tcacatagaa ctgcgatgac cgggctgtaa agcctgacga ggcgccaata ccccatacga
    25681 agctcgagct aatttgattt ttggagaata gacatgtccc gagtctgcca agttactggc
    25741 aagcgtccgg tgaccggtaa caaccgttcc cacgcactga acgcgactaa acgccgtttc
    25801 ctgccgaacc tgcactctca ccgtttctgg gttgagagcg agaagcgttt tgtcaccctg
    25861 cgcgtatctg ctaaaggtat gcgtgtaatc gataaaaaag gcatcgatac agttctggct
    25921 gaactgcgtg cccgtggcga aaagtactaa gtacttagag gaaataaatc atggctaaag
    25981 gtattcgtga gaaaatcaag ctggtttctt ctgctggtac tggtcacttc tataccacta
    26041 cgaagaacaa acgtactaag ccggaaaaac tggaactgaa aaaattcgat ccagttgttc
    26101 gtcagcacgt gatctacaaa gaagcgaaaa tcaaataatt ctcgctttga tgtaacaaaa
    26161 aacctcgctc cggcggggtt ttttgttatc tgcttgcccc catattgatt gcatctgttc
    26221 attcctggag atgctatgcc tgaattaccc gaagttgaaa ccagccgtcg cggcatagaa
    26281 ccgcatctcg ttggtgcaac cattcttcat gcagtggtgc gcaacggacg cttgcgctgg
    26341 ccggtttcag aagagatcta ccgtttaagc gaccaaccag tgcttagcgt gcagcggcgg
    26401 gctaaatatt tgctgctgga gctgcctgag ggctggatta tcattcattt ggggatgtct
    26461 ggcagcctgc gcatccttcc agaagaactt ccccctgaaa agcatgacca tgtggatttg
    26521 gtgatgagca acggtaaagt gctgcgctac accgatccgc gccgctttgg tgcctggctg
    26581 tggaccaaag agctggaagg gcataatgtg ctggcccatc ttggaccgga gccgctcagc
    26641 gacgatttca atggtgagta tctgcatcag aagtgcgcga agaaaaaaac ggcgattaaa
    26701 ccgtggctga tggataacaa gctggtggta ggggtaggga atatctatgc cagcgaatca
    26761 ctgtttgcgg cggggatcca tccggatcgg ctggcgtcat cactgtcgct ggcagagtgt
    26821 gaattgttag ctcgggtgat taaagcggtg ttgctgcgtt cgattgagca gggcggtaca
    26881 acgctgaaag attttctgca aagtgatggt aaaccgggct atttcgcaca ggaattgcag
    26941 gtttacgggc gaaaaggcga gccgtgtcgg gtgtgcggta cgccgattgt ggcgactaaa
    27001 catgcgcagc gggcaacgtt ttattgtcgg cagtgccaga agtaattcat gcgcgccgga
    27061 tggcatacca tccggcataa acgctacgct aacttcgcca tcagcgcctg atggacattc
    27121 tccggcagga aatgggtgac atcgccctga tggcgcgcca cctctttcac caacgatgaa
    27181 gagataaacg accactcttt cgacggcatc agaaacacac tttccagttc cggcattaag
    27241 tggcgattca tatgcgccag ctgcatttca tattcaaaat ccgccaccgc acgcaggcca
    27301 cgaatcagca ccgtagcgtg ttgattacgg gcgaagttcg ccatcaaatc actaaacccg
    27361 accacttcca cgttccccag atgcgcggtt gcctgctgtg ccagcgccac acgctcttcc
    27421 agggtaaaca tcggtttttt actggggctg gcggcaatcg ccagaataac gtgatcgaac
    27481 atttgcgtgg cgcgcgtcac gatatcgata tgaccattgg taatgggatc gaaagtaccc
    27541 ggataaatcg cccgtttttg cataacaacc tcaatgcgtt ttcggtggca ggtaaggttc
    27601 cagcagttga agcagacgct gtagcgcgcc ctggttttga tacagtactt caacggcatg
    27661 acggccatag aaactacggt aatcggcgtc ggtgagtaaa gaggacacct cttttgcaag
    27721 cgtagtggca tcggtaacgg taatcagccc gcttgcctgc tccagccgcg cgcaaatgtc
    27781 tttaaagtta aaagtatgcg gccccatcaa taccggaata gcgtgtgcgg cagcttccag
    27841 cggattatgc ccaccacgtt caaccagtga accgccaaca aaggcgagat cggcaatgcc
    27901 gtacagtaac atcaactcgc ccatcgtatc gccaaccaca acctgcgtgc tggtggaggg
    27961 gacttcccct gaagagcgtg tgatatagct tagtccagcc tggcggacaa gattaatcgc
    28021 atccgggaag cgttctggat gacggggtac caggatgagc aataaattcg ggaattgctg
    28081 taacaatgcc tgatgtgcgg cgatcaccac actctcttcg ccttcgtgag tgctggtggc
    28141 aatccatacc gggcggtgtg gtgcccactg gcggcgcagc gtcacggctt tagcagccaa
    28201 ctgcggcgtt acagaaatat cgaatttcag gctaccggta acggtcacct gattattttt
    28261 tgcgcccagc gccacaaaac gtgcaccatc ttcttcattt tgcgcagcaa tcagcgtaat
    28321 acgacgcagc aagcgacgga cgaatttacc cagtttggca taacctgcgg ccgagcgggc
    28381 agagagtcgc gcgttagcga tcaccagcgg aattttacgt ttgtgtagcg ccgcaatcag
    28441 gttaggccat agttcggttt ccataatcaa caccagtttg gggtcgactt tattcaggaa
    28501 acggttgagc gcatcgggca ggtcatacgg cagataaacg tgctgaacat ccttcccgaa
    28561 agccgattgt acgcgctccg aaccggttgg cgtcatggtt gtaacggtaa tcggtaaatc
    28621 aggataacga tggcgcagcg cgcgcaccaa cgggatcgcc gccagagttt caccgacgga
    28681 gacggagtgc agcataatgc cgcctggttt tagcggatgg cggtaaaaac cgtaacgttc
    28741 accccagcgt tttcgatagg ccggagcctt acgtccgcgc acccagagcc gtatccagat
    28801 cagcggctga ataaggtaga gaagggcggt gtaaagcaat tcgagcatag taaatagctg
    28861 acttatggat gtgctgggga ttctatgtat ttagctgtgg ctttaccatt acttttcccg
    28921 tttttgactt aaatagcaat ttacccgcca attcttgctg aacatctata ctttttttgt
    28981 atttttatgc gattcattga tttctgctgt acgtgcagga aggaatcgca gagcaacagt
    29041 gaaagaatat ggttgctaat gattttattt gattgatttc tttattgaaa atcaacaatg
    29101 tgcttaaaaa tcattcgggt cggattgccg cgaaagaagt gatacactag cgcgtcaata
    29161 tcagtgcggt atcagtattc aggtagctgt tgagcctggg gcggtagcgt gctttttctg
    29221 cttcacctta atcggataat ctcaacaaaa agagttactt gtggataagc catttcgaag
    29281 aattttgctc attaagatgc gttttcatgg ggatatgtta ttaactactc ccgtcattag
    29341 ttcgctgaaa aaaaattacc ctgacgcaaa aatcgatgtg ctgctttatc aggacaccat
    29401 cccgatcctg tctgaaaatc cagagattaa cgcgctctac ggcataaaaa ataaaaaagc
    29461 aaaagcctca gaaaaaattg ccaacttttt tcatctcatc aaggtattac gtgccaataa
    29521 gtatgacctt atcgtcaatc tcaccgatca atggatggtt gctatactgg ttcgcttatt
    29581 aaatgcccgt gtgaaaattt cccaggatta tcatcatcgg cagtctgctt tttggcgtaa
    29641 aagtttcacc catttggtgc cgttgcaggg tggaaatgtg gtggaaagta acttatccgt
    29701 gctgacccca ttgggagttg attcgttggt gaagcagaca accatgagtt acccgcctgc
    29761 aagctggaaa cgtatgcgtc gcgaacttga tcacgctggt gttggacaaa attatgtggt
    29821 tatccaacct acggcgcggc aaatcttcaa atgctgggac aacgccaagt tttccgctgt
    29881 gattgatgcc ttacatgctc gtggttatga agtcgttctg acgtccggcc cagataaaga
    29941 cgatctggcc tgcgtcaatg aaattgcgca gggatgccag acgccaccag taacggcgct
    30001 ggctggaaag gtgaccttcc cggaacttgg tgcgttaatc gatcatgcgc agctgtttat
    30061 tggcgttgat tccgcaccgg cgcatattgc cgctgcagtt aatacgccgc tgatatcgct
    30121 gtttggtgcg acagatcata ttttctggcg tccctggtca aataacatga ttcaattctg
    30181 ggcgggagat taccgggaaa tgccaacgcg cgatcagcgt gaccgaaatg agatgtatct
    30241 ttcggttatt ccggcggcag atgtcattgc tgctgtcgat aaattactgc cctcctccac
    30301 gacaggtacg tcgttatgat cgttgctttt tgtttatata aatattttcc ctttggcggt
    30361 ttgcagcgtg attttatgcg tattgctcag acagtcgccg cccgaggtca tcatgttcgg
    30421 gtttataccc agtcgtggga aggcgaatgc cctgatgtat ttgaactgat caaagtgccg
    30481 gttaaatcgc ataccaatca cgggcgcaat gcggagtatt ttgcctgggt gcaaaaacat
    30541 ttacgcgaac atcccgtcga taaagtcgtt ggattcaaca aaatgccggg gctggacgtt
    30601 tattatgccg ctgatgtttg ttatgccgag aaagtagcgc aggaaaaagg ctttttctat
    30661 cgcctgacgt cacgttatcg ccattatgcc gcctttgagc gggcaacctt cgaacagggt
    30721 aagccgacac agctgctgat gctgacagat aagcaaatcg ccgatttcca gaaacattat
    30781 cagactgaag cggagcgttt tcatattctg ccaccgggga tttatcctga tcgtaaatat
    30841 agccagcagc cagccaatag ccgtgaaatc ttccgtaaga agaatggaat aaccgaacaa
    30901 caatatttat tgttgcaggt cggttcagac ttcacgcgta aaggtgtcga tcgttccatt
    30961 gaagcacttg cttcgttacc ggattcgctg cgccacaaca cattgctata tgttgttggg
    31021 caggataaac cgcgaaaatt tgaggcgctg gcagaaaaac gcggcgtgtg cagtaatgtt
    31081 cactttttct cggggcgcaa tgatgtctcg gaattaatgg cggcggcgga tttattactg
    31141 catcctgcct accaggaagc ggcgggaatt gtgctgctgg aagcgattac tgcaggatta
    31201 ccggtactaa caacagccgt ttgtggctat gcgccttata ttgtcgaggc taattgcggc
    31261 gaggctattg ctgagccatt ccgccaggaa acattgaatg agattttacg caaagcgtta
    31321 acgcaatctt cattgcgcca ggcctgggcg gaaaatgcgc gatattatgc tgatacacaa
    31381 gatttataca gtctgccaga gaaagcggcg gatatcataa cgggtggtct ggatggttga
    31441 acttaaagag ccgtttgcca cgttatggcg cggcaaagat ccttttgagg aagttaaaac
    31501 cttgcagggt gaggtatttc gtgaactgga aactcgccgt actctgcgct ttgaaatggc
    31561 gggcaaaagc tattttctca aatggcatcg cggcacgacc ctgaaagaga taatcaaaaa
    31621 tttactctca ttgcggatgc cagtattagg cgcagaccgc gaatggaatg cgattcatcg
    31681 actgcgggat gtcggcgttg atactatgca tggggtggca tttggcgaaa aaggcatgaa
    31741 tccgctgacc agaacttcat ttattattac cgaagatctg acaccaacca taagtctgga
    31801 agattactgt gctgactggg cgactaaccc tccagatgtt cgcgtaaagc gtatgcttat
    31861 taagcgtgtc gcgacgatgg tgcgcgatat gcatgctgcg ggcattaacc accgtgactg
    31921 ttatatctgt catttcctgc tgcacttgcc tttttccggt aaggaagagg agttaaaaat
    31981 ttcggtaatt gacctgcacc gggcgcagct tcgcacgcgc gttccacgtc gttggcggga
    32041 taaagatctt attgggcttt atttttcttc gatgaatatc ggcctgactc agcgggatat
    32101 ctggcggttt atgaaagtgt attttgccgc cccgcttaaa gacattctca agcaggaaca
    32161 aggactgctg tcgcaagcag aagcaaaagc cacaaaaatc agggaaagaa cgattcgaaa
    32221 atcgttgtaa cccgtaactt atttttgcca aaattttgga tacagaataa atatgtctca
    32281 actcaatgat agtgacatca tcctttttga gtataatttt cattatcaaa atataagatc
    32341 taaaaatact cttgatatag catttggtat tgacagaaat tttctt
//
LOCUS       DAZGJW010000046        32194 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZGJW010000046 DAZGJW010000000
VERSION     DAZGJW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.046"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(130..1359)
                     /locus_tag="ACHKIK_002106"
     CDS             complement(130..1359)
                     /locus_tag="ACHKIK_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9083276.1"
                     /translation="MSRAMNKLSDTLLRKLHGKPAEKNTFYSDGGNLSVKYLTSGKLT
                     WYFTYRAGTGRETRPERIKLGSYPDLSLKAAREKAAQCRTWLAEGKNPRHEMNYTVQE
                     ALKPVTVGDALTYWLEYYVKENRVDYIALKRRLNNHVIQQIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFVLQACKQALKFCRRRRYAISNVLDDLNVADVGKKPDISERVLSNK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGARTVELRLSEIGEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNVLDMWMERLGI
                     LAGTHENVTTLPVARRK"
     gene            1347..1529
                     /locus_tag="ACHKIK_002107"
     CDS             1347..1529
                     /locus_tag="ACHKIK_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083277.1"
                     /translation="MLCSRFTPSLRKSDYTSDYTDRCTKGYNVKQAGTRKITNLLIIK
                     NIMHSLKHYETAKNTK"
     gene            1536..2441
                     /gene="tqsA"
                     /locus_tag="ACHKIK_002108"
                     /pseudo
     CDS             1536..2441
                     /gene="tqsA"
                     /locus_tag="ACHKIK_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            complement(2466..3854)
                     /gene="pntB"
                     /locus_tag="ACHKIK_002109"
     CDS             complement(2466..3854)
                     /gene="pntB"
                     /locus_tag="ACHKIK_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO9083278.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(3865..5397)
                     /gene="pntA"
                     /locus_tag="ACHKIK_002110"
     CDS             complement(3865..5397)
                     /gene="pntA"
                     /locus_tag="ACHKIK_002110"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO9083279.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            5921..6865
                     /gene="ydgH"
                     /locus_tag="ACHKIK_002111"
     CDS             5921..6865
                     /gene="ydgH"
                     /locus_tag="ACHKIK_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO9083280.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            7051..8433
                     /gene="ydgI"
                     /locus_tag="ACHKIK_002112"
     CDS             7051..8433
                     /gene="ydgI"
                     /locus_tag="ACHKIK_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9083281.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            8470..9192
                     /gene="folM"
                     /locus_tag="ACHKIK_002113"
     CDS             8470..9192
                     /gene="folM"
                     /locus_tag="ACHKIK_002113"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO9083282.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(9189..9524)
                     /gene="ydgC"
                     /locus_tag="ACHKIK_002114"
     CDS             complement(9189..9524)
                     /gene="ydgC"
                     /locus_tag="ACHKIK_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO9083283.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            9653..10372
                     /gene="rstA"
                     /locus_tag="ACHKIK_002115"
     CDS             9653..10372
                     /gene="rstA"
                     /locus_tag="ACHKIK_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO9083284.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            10376..11677
                     /gene="rstB"
                     /locus_tag="ACHKIK_002116"
     CDS             10376..11677
                     /gene="rstB"
                     /locus_tag="ACHKIK_002116"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO9083285.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            11753..12682
                     /gene="tus"
                     /locus_tag="ACHKIK_002117"
     CDS             11753..12682
                     /gene="tus"
                     /locus_tag="ACHKIK_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO9083286.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12679..14082)
                     /gene="fumC"
                     /locus_tag="ACHKIK_002118"
     CDS             complement(12679..14082)
                     /gene="fumC"
                     /locus_tag="ACHKIK_002118"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO9083287.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAVSLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(14225..15871)
                     /gene="fumB"
                     /locus_tag="ACHKIK_002119"
     CDS             complement(14225..15871)
                     /gene="fumB"
                     /locus_tag="ACHKIK_002119"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO9083288.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            16070..17245
                     /gene="manA"
                     /locus_tag="ACHKIK_002120"
     CDS             16070..17245
                     /gene="manA"
                     /locus_tag="ACHKIK_002120"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO9083289.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSILQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            17346..18854
                     /gene="ydgA"
                     /locus_tag="ACHKIK_002121"
     CDS             17346..18854
                     /gene="ydgA"
                     /locus_tag="ACHKIK_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO9083290.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(18899..20164)
                     /gene="uidC"
                     /locus_tag="ACHKIK_002122"
     CDS             complement(18899..20164)
                     /gene="uidC"
                     /locus_tag="ACHKIK_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO9083291.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(20203..21576)
                     /gene="uidB"
                     /locus_tag="ACHKIK_002123"
     CDS             complement(20203..21576)
                     /gene="uidB"
                     /locus_tag="ACHKIK_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO9083292.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(21573..23384)
                     /gene="uidA"
                     /locus_tag="ACHKIK_002124"
     CDS             complement(21573..23384)
                     /gene="uidA"
                     /locus_tag="ACHKIK_002124"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO9083293.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     AEQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(23773..24363)
                     /gene="uidR"
                     /locus_tag="ACHKIK_002125"
     CDS             complement(23773..24363)
                     /gene="uidR"
                     /locus_tag="ACHKIK_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO9083294.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAERMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(24584..25351)
                     /gene="hdhA"
                     /locus_tag="ACHKIK_002126"
     CDS             complement(24584..25351)
                     /gene="hdhA"
                     /locus_tag="ACHKIK_002126"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO9083295.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(25463..26491)
                     /gene="malI"
                     /locus_tag="ACHKIK_002127"
     CDS             complement(25463..26491)
                     /gene="malI"
                     /locus_tag="ACHKIK_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO9083296.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            26666..28258
                     /gene="malX"
                     /locus_tag="ACHKIK_002128"
     CDS             26666..28258
                     /gene="malX"
                     /locus_tag="ACHKIK_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO9083297.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVLALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            28268..29440
                     /gene="malY"
                     /locus_tag="ACHKIK_002129"
     CDS             28268..29440
                     /gene="malY"
                     /locus_tag="ACHKIK_002129"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO9083298.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRLLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            29544..30545
                     /gene="add"
                     /locus_tag="ACHKIK_002130"
     CDS             29544..30545
                     /gene="add"
                     /locus_tag="ACHKIK_002130"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO9083299.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(30580..31620)
                     /gene="ydgJ"
                     /locus_tag="ACHKIK_002131"
     CDS             complement(30580..31620)
                     /gene="ydgJ"
                     /locus_tag="ACHKIK_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO9083300.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            31863..31988
                     /gene="blr"
                     /locus_tag="ACHKIK_002132"
     CDS             31863..31988
                     /gene="blr"
                     /locus_tag="ACHKIK_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO9083301.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
BASE COUNT         7865 a         8400 c         7765 g         8164 t
ORIGIN      
        1 aatcagctac ttataaatct gtacactttt tcgcctcttg ttcgtagttg ttccgcattg
       61 ttgctcattg ttgcacgttg tatctgttcg catatccagt atgcgcatac tgaaaaaata
      121 cgaaaaaaat tattttcttc tggctactgg tagcgtggtt acgttttcat gtgttcccgc
      181 cagtatccct aaccgctcca tccacatatc cagcacattg cgtttagcat ccagataacg
      241 ggaatgatta taaactcgct gcattcctgg catctggtgg cctgtaagct gctccacgac
      301 atgcggatca acgcctaaat cgttcagcat ggttgtaaag gtgcgccgga tgtcatgcag
      361 tgaccagtga ggatgattaa gcctcctgtg cgctaatctt ccatactgcg atacgctggc
      421 ctcctgtttc acttccccca gcaataagcc cgtgtgcctg ttctgctcca ccagctgcgt
      481 gacgaacggc aggatcgctt ccggtatggg ccggaatatt gcgaccttcg ttttgctgtg
      541 ctccttcgga acggtccata gcatttccgt aaaatcccac tccccgatct ccgatagcct
      601 cagttctacc gtcctggctc cgaagacaat caggaggcgg attaacgcga cgtagtaagg
      661 ggaaaatatt tttttgtcca gtgcctgcaa taattcgcca agttctttgt tacttaagac
      721 acgttcgctt atatccggtt ttttcccaac gtccgccacg ttcagatcgt ccagaacgtt
      781 gctgattgca tagcgccgcc tacggcagaa cttaagcgcc tgtttgcacg cctgtagcac
      841 gaatccggca gtaacaggcg ttcgctttgc cacctggtca aaacaggcca gccagtgccg
      901 tagctcgcat ttatccagcg gcatagcacc aatctgctgt attacgtgat tattaagtcg
      961 ccttttcagg gcgatataat ccacgcggtt ttcctttacg taatactcaa gccagtaggt
     1021 gagcgcatcg ccaaccgtta cgggctttaa cgcttcctgt acggtgtaat tcatctcatg
     1081 acgtggattt ttcccctcag ccagccatgt gcgacactgt gcggcttttt ccctggctgc
     1141 tttcaggctc agatcaggat aacttcccag cttaatgcgt tcgggtcgtg tctcccttcc
     1201 cgttccggcc ctgtatgtga aataccaggt caatttccct gatgttaaat atttcacgct
     1261 caggtttccg ccatcactat aaaacgtgtt tttctccgct ggcttaccat gaagtttcct
     1321 tagcaaggta tcgctcagtt tgttcattgc tctgctcacg ttttaccccc tctttacgaa
     1381 aaagtgacta caccagtgac tacaccgatc gttgcacaaa gggctacaac gtgaaacaag
     1441 ctggaacaag gaaaatcaca aatctgttga taatcaaaaa cataatgcac agcctgaaac
     1501 attatgaaac agcaaaaaac actaaatgat aaaatgctgt tattcttaac ccgctggtgc
     1561 aacacatggt ccgctggcga gtgccgcgtg tactggcggt gtcgattttg atgaccatca
     1621 tcgtgatggc gatggtgttg ctattagctt atctgggttc cgcgctcaac gagttgacgc
     1681 ggacgttacc gcaatatcgc aactctatta tgacgccgct gcaagctctt gagccgttgt
     1741 tgcaacgcgt agggattgac gtctcagttg accagctggc gcattacatt gatccgaacg
     1801 cggcgatgac gttgctcacc aacttattga cgcagttatc taatgccatg tcatcaatat
     1861 ttttattgct gctgacggtg ctgtttatgc tgctcgaagt gccacaattg cccggaaaat
     1921 ttcagcaaat gatggcgcgt ccggttgaag ggatggcggc gattcaacgt gcaattgaca
     1981 gtgtttctca ttatctggtg ctgaaaacag ccatcagcat catcaccggc ctggtcgcct
     2041 gggcgatgct cgccgcactc gatgttcgct tcgcttttgt ctggggattg ctggcctttg
     2101 cgcttaatta catccctaat attggttcag ttctcgcggc aatcccccct atcgctcagg
     2161 tactggtgtt taatggcttc tacgaagcgt tgctggtgct ggcgggatat ctgctgatta
     2221 atctggtctt cggcaatatt ctggagccgc gcatcatggg acgtgggctg gggctttcca
     2281 cattggtggt atttttgtcg ttgatttttt ggggatggtt gttaggaccg gtgggtatgc
     2341 tgctttccgt gccgttaaca attattgtca aaattgcgct tgaacaaaca gcgggaggtc
     2401 aaagcatcgc cgttctgtta agcgatctca ataaagagtg acggcctccg cagaggccgt
     2461 cagggttaca gagctttcag gattgcatcc acgctggctt tggcgtcacc aaacagcatg
     2521 tgggtgtttt ccttgaagaa cagcgggttt tgcacaccag catagccagt gttcatcgaa
     2581 cgtttaaaga caatcacgtt ctgcgctttc cacacttcca gcacaggcat accagcaata
     2641 ggactcttcg gatcatcctg cgccgccggg ttaaccgtat cgttagcacc aatcaccagt
     2701 acggtatcgg tatcagcaaa gtcatcgttg atctcgtcca tttccagcac gatgtcatac
     2761 ggtacttttg cttcagccag caatacgttc atatgtccag gcaaacgccc cgcaaccggg
     2821 tggataccga aacgcacgtt gataccacga gcgcgcagtt tctcggtaat ttcagcgaca
     2881 ggatattgcg cctgcgcgac tgccatgccg taccccggag taatgatcac tgaatgggag
     2941 tttttcagca gttccgctgt ctcttctgcg gtgatttcgc ggtgctcgcc cacttcctga
     3001 tcatcgccag tagaagagcc gtcggtgccg aaaccacccg caataacgct gataaaggaa
     3061 cggttcatcg ccttacacat aatgtaagaa aggatagcgc ccgaagaacc gaccagcgca
     3121 ccggtcacaa tcagcaggtc gttgctgagc ataaagcccg cagccgcagc cgcccagccg
     3181 gagtacgagt tcagcatcga caccaccact ggcatatctg caccaccgat ggaggcgact
     3241 aaatgccagc cgaataccag cgcaatggcg gtcattatca gcaatgccag cacttgcagg
     3301 ccgacgctat ccgtgcgaac aaatacaatc agcagcagga aggaaacgac cagagccgcc
     3361 aggttcattt tgtgacggtt tggcagcatc aacggtttag acgaaatctt gccacacagt
     3421 ttgccgaacg ccaccaccga acccgtgaac gttaccgccc cgatgaagat accgaggaac
     3481 acttccgtca ggtgaatatt gaccagaatc ggtgccattc ccgcgtcatg atgcagataa
     3541 ctgttaaagc caaccagcac tgccgccaga cccacgaagc tatgcaggat cgccaccagt
     3601 tctggcattt cggtcatttc aactttcttc gccagacgga taccaattgc cccaccaatg
     3661 accatcgcca gcaagatcca gccaacatta cccgtatccg gtccaaaaat ggttgcgatt
     3721 aacgcaatcg ccatcccggc gataccgaag ttgttaccct ggcgagacgt ttcatgtttc
     3781 gaaagaccgg ccagactgaa gataaacagg atcgcggcaa caatgtatgc agctgtaact
     3841 aatcctccag acatatgtta ccccttaatt tttgcggaac attttcagca tgcgctgagt
     3901 cacggtgaag ccaccgaaaa tattaatgct ggctataagc accgcgataa aactaaggaa
     3961 gctaacccag ccgccctggc caatctgcaa cagtgctccg acaacaataa tccctgaaat
     4021 cgcgttggtg accgacatca acggtgtatg cagcgcatgc gatacattcc acaccacgta
     4081 ataaccgaca acgcaggcca gcgcgaaaac ggtgaagtgc ccaagaaact ctttcggcgc
     4141 aacgcttgcc agccaaccga aaaggatgat tgccagcgcc atcaatgcgt atttacgcca
     4201 cggtgagcag gcacattttt cctcagtttt cacttccggt gccgcttttt gtgccgcctg
     4261 cggctgagct gatacctgaa tcggcggtgc cggccaggta atttcgcccg cacggatcac
     4321 ggtcacgccg cgaatcacca catcatcaaa atcaacagtg atattgccgt ctttctcttt
     4381 gcacaacagt ttcagcagat taacgaggtt agtaccgtaa agctgtgagg attgcgtcgg
     4441 cagacggccc ggaagatcgg tataaccaat cactttgaca ccattttccg tagtgaagat
     4501 ttcacccggc acggtgtatt cacagttgcc accgttttgg gctgccaggt cgacaatcac
     4561 actgcccgcc ttcatggagt caaccatttc acgggtaatt agcttcggcg ctggtttgcc
     4621 tggaataagc gcggtggtga caatgatatc gacctctttt gcctgggcgg caaagagttc
     4681 catttccgct ttgatgaacg cgtccgacat cactttggca tagccatcgc cgctgcccgc
     4741 ttcctcttta aaatccagct cgaggaattc cgcgcccata ctttgaactt gttctttcac
     4801 ttccgggcgg gtgtcgaatg cacgcacaat cgcgccgaga ctgtttgctg cgccaatggc
     4861 ggccagacct gcaacacccg caccaatcac catcactttt gccggtggca ctttcccggc
     4921 cgcagtaatt tgcccggtaa agaagcgccc aaattcatgt gccgcttcaa caatggcgcg
     4981 ataaccggcg atgttcgcca tcgagcttag tgcgtccagc gattgtgcgc gtgagatacg
     5041 cggcacagaa tccatcgcca tcacggtcac gttacgttcc gcaagttttt gcattaattc
     5101 cggattctgc gcaggccaga taaaactcac cagcgttgtc ccaggattca gtaacgcaat
     5161 ttcatcatct aacggcgcat tgaccttcag aatgatctct gactgccaga cgctattccc
     5221 ttctacaatt tcagcgcccg cttgcacaaa cgctttatcg tcaaaacttg ccagttgacc
     5281 cgcgccgctc tctaccgcga cggtaaaacc cagtttcagc agctgttcca ctgtttttgg
     5341 cgttgctgca acacgggttt cattggttaa ccgttctctt ggtatgccaa ttcgcatgat
     5401 attcccttcc atcggtttta ttgatgatgg tttgcctgtg tcaggagcca cacaagccgc
     5461 tcatgtacga gctaaatgtt actccgttaa aataaattag taacaaacgc cctataacgt
     5521 actgaaaatt atgcctgtga tctagcgcca aaaatcagta tttcggcgtg aactcgcaaa
     5581 atattaacga ttcagccgtg atagtgggat aaacacctta gaacgccgga taaagactga
     5641 taattgtctt cgacggtcgg gtaaaacgag acatcgcccc ggcacgaatc actacttaac
     5701 attaaattaa cttatacaat tcagttgctt cagtagtaat gatgctgata cggctgtttt
     5761 ttaagcatag acggtcattt gagcaggatt aaaattggct taaggaatgt gatatgaaaa
     5821 atgacgcaga cagttacacc gtttaaatgc aataatcagc cacgtttctc gttaataaca
     5881 ataccagtac ctggtttgcg caaggcgaag gattattttt atgaagctta agaacaccct
     5941 cctggcgtcg gcactgcttt ctgctacggc attctccgtt aacgcagcaa cagaactgac
     6001 accggagcaa gcggcagcgg ttaaaccttt tgaccgtgta gtggttaccg gtcgttttaa
     6061 tgctattggc gaagcggtga aagccgtttc tcgtcgcgca gataaagaag gtgccgcctc
     6121 tttttatgtt gtcgacactt ctgattttgg taacagcggt aactggcgtg tggtcgctga
     6181 cctctataaa gccgatgctg aaaaagcaga agaaacaagt aatcgcgtaa ttaacggtgt
     6241 tgtcgaactg ccgaaagatc aggctgttct gattgaaccg tttgacacgg tcaccgtcca
     6301 gggcttctat cgtagccagc cagaagtcaa tgatgccatc accaaagcag caaaagcgaa
     6361 aggtgcctac tctttctaca ttgttcgtca aatcgatgcc aaccagggcg gcaaccagcg
     6421 tattactgca ttcatctata aaaaagatgc taagaaacgt atcgtccaga gcccggatgt
     6481 gatcccggca gattccgaag caggacgtgc agctctggct gccggtggcg aagccgcgaa
     6541 gaaagttgag atcccaggtg ttgcgactac cgcatcacca agttctgaag tcggtcgttt
     6601 ctttgaaacc cagtcatcaa aaggcggacg ttacactgtc acgcttcctg acggcactaa
     6661 agtcgaagaa ctgaacaaag cgacagcagc gatgatggtc ccgttcgaca gcatcaaatt
     6721 ctctggcaac tacggcaaca tgaccgaagt ctcctatcag gttgcgaaac gtgccgcgaa
     6781 gaaaggtgct aagtattacc acatcacccg ccagtggcag gaacgtggta ataacctgac
     6841 cgtcagcgca gatctgtata aataactatt cagttactcc tataaggcgg cttgatgccg
     6901 cctttcgcat ttttagtcac aaatttcccc gcgaccattg catgcagaca catctcttcg
     6961 taaaatcccg cgcttcagtg gcaaactcca tttttatgcg ttttgcccta atatttattc
     7021 ttcttatcac gttttaatca ctggatatcg atggaaaaga aactgggact gagcgcactc
     7081 accgcgctgg tattaagctc aatgctgggc gcgggtgttt tcagtctgcc gcaaaatatg
     7141 gcggcagttg ccagcccggc agcactgctc atcggctggg gtattactgg cgctggcatt
     7201 ttattgctgg cctttgccat gctgatcctc acgcgcattc gtcccgaact tgacggcggt
     7261 atctttacct atgcccgcga agggtttggc gagctaatcg gcttttgttc cgcatgggga
     7321 tactggctgt gcgcagtcat cgccaacgtc tcctatctgg tgatcgtttt ttccgcgtta
     7381 agctttttta cggacacgcc ggaattgcgc ctgttcggcg atggtaatac ctggcaatcg
     7441 atagtcggtg catcggcttt attgtggatt gttcactttt tgatcctgcg cggggtgcaa
     7501 accgctgcca gcattaacct ggtggcgaca ctggcaaaat tgttgccgct gggtctgttt
     7561 gttgtgctgg cgatgatgat gttcaaactg gataccttca agctcgactt caccggactt
     7621 gcacttggcg tacccgtgtg ggaacaggtg aaaaacacca tgctgatcac cctgtgggtt
     7681 ttcattggtg tggaaggtgc cgtggttgtt tctgcgcgtg cgcgtaataa acgtgatgtt
     7741 ggcaaagcga cactgctggc ggttctctcc gctctgggcg tttacttgct ggtaacgctg
     7801 ctttcgctgg gcgtggtggc acgtcctgaa ctggctgaaa ttcgtaaccc gtcaatggcc
     7861 ggtctgatgg tggaaatgat gggaccatgg ggcgaaatca tcatcgctgc cggtttgatc
     7921 gtttccgttt gcggcgcgta cttaagctgg accatcatgg cggcagaagt tccgttcctg
     7981 gctgccactc ataaagcatt cccacgcatt tttgcgcgcc agaacgcaca agctgcgcca
     8041 tccgcctcgc tgtggttgac caacatctgc gtgcaaattt gtctggtact catctggctg
     8101 accggttccg attacaacac gttgctgacc atcgcctcag aaatgattct ggtgccctat
     8161 ttcctggtcg gcgcgttctt gctgaaaatc gcaacccgtc cactgcataa agcggtaggt
     8221 gtcggtgcct gcatttatgg cttatggtta ttgtatgcct ctggtccaat gcacttgctg
     8281 ttatccgttg ttctgtatgc gccggggctg ctggttttcc tgtatgcgcg caaaacgcat
     8341 acacatgata atgtactgaa ccgtcaggaa atggtgctga ttggcatgct gttaattgcc
     8401 tcagttccgg cgacctggat gctggtggga taacgttcat accatcgttg gatagcaaag
     8461 gagataacga tgggtaaagc ccagcccttg ccaatattaa ttactggcgg aggtcgtcgc
     8521 atcggcctcg ccctcgcctg gcatttcatt aatcaaaagc aaccggtgat tgtcagctat
     8581 cggacgcact atccagccat tgatggactg attaaggcag gtgcgcagtg tattcaggct
     8641 gatttttcga ccaacgacgg tgtgatggcg tttgccgatg aagtactaaa aagcacccat
     8701 ggtctgcgtg ctattttgca taacgccagt gcgtggatgg cggaaaaacc aggtacacca
     8761 ctggccgacg tactggcttg catgatgcag atccacgtta ataccccata cctgctcaac
     8821 catgcgctgg aaagattact gcgtgggcac ggacacgccg ccagcgatat cattcacttt
     8881 accgattatg tggtggagcg cggtagcgac aaacatgttg cgtatgctgc aagcaaagcg
     8941 gcactggata atatgacccg ctcgtttgcc cgcaagctgg caccggaagt gaaagtgaat
     9001 tctattgcgc catcgctgat cctgtttaat gaacatgatg atgccgaata tcgacaacag
     9061 gcgctgaata aatcactgat gaaaaccgcg cctggcgaga aagaagtgat cgacctggtc
     9121 gattacttac ttaccagctg ctttgtcacc ggacgcagtt tcccacttga tggcggtcgt
     9181 catctgcgtt aatgcagctt tatccagcat ataatcaaca cccatgcgct tatcccccaa
     9241 cacgcaacag aaccaacaaa agcggcgggc aagcgcatca ttccggtgaa ataccacagc
     9301 gacaccaggt aaacaaaata gggaataatc gaccacatac taaaaatgat ggttgcgcgt
     9361 aaggcttcaa tgccgcgttc gctggcaaca atataatgcg cgataagcgc aaaggtcggg
     9421 aaaagtggaa tcagcccggc gatataataa tttttcgttt ttgctaaaac accaatcaac
     9481 agcactacca gcgcaccgag cgcggctttg attaccagcc ccatcttttt accttaacac
     9541 ttccataaca agtcatcagt agaatacctg atgaaaactt gtttagaaac gattgatagt
     9601 aagtaaaaac agcgcggtgt attgtgacgt ttttatatct accgtgaatg ttatgaacac
     9661 tatcgtattt gtggaagatg atgcggaagt cggttcactg attgccgcgt acctggcaaa
     9721 acatgatatg caggttaccg tagaaccgcg cggcgaccag gccgaagaaa ccattttgcg
     9781 agaaagtccg gatttggtgt tactcgacat catgctacca ggcaaggacg gcatgaccat
     9841 ttgtcgtgat ttacgcgcaa agtggtctgg accgattgtt cttctaacct ctctcgatag
     9901 cgatatgaac cacatcctgg cactggaaat gggtgcctgc gactatattc tcaaaacgac
     9961 gccccctgct gttttgctag cgcgtttacg tttgcatttg cgtcagaatg agcaagccac
    10021 actgaccaaa ggtcttcagg aaacgtctct gactccctac aaagccctgc atttcggcac
    10081 gttgaccatc gatcccatca accgcgtagt caccctggct aacactgaaa tctcgctctc
    10141 gacagctgat ttcgaattat tgtgggaatt agctacccat gccgggcaaa tcatggaccg
    10201 cgatgcattg ctgaaaaatt tacgcggcgt cagttatgac ggactggatc gtagcgtgga
    10261 cgtggctatt tcgcggttaa gaaaaaaact gctcgataac gccgcagaac cttatcgcat
    10321 taaaactgtg cgtaacaaag gctatctttt tgcgcctcat gcatgggaat aagcgatgaa
    10381 aaaactgttt atccagtttt acctgttatt gtttgtctgc ttccttgtga tgtctctgct
    10441 ggttgggttg gtgtacaaat ttaccgccga acgcgcgggc aaacagtcgc tggatgattt
    10501 gatgaacagt tcgctgtatc tgatgcgcag cgaattgcgt gagatccccc cacacgactg
    10561 gggtaagacg ctgaaagaga tggatttaaa tctctctttc gatctgcgtg tcgagccact
    10621 gagtaaatac catcttgatg atatttccat gcaccgactg cgtggcggcg aaattgtcgc
    10681 cctggacgat cagtacacgt ttttgcagcg tatcccgcgc agccactacg tgctggcagt
    10741 tggtcctgtt ccttatcttt attatctcca tcagatgcga ttgctggata tcgccctgat
    10801 cgcttttatt gctatttccc tcgcctttcc ggtgtttatc tggatgcgtc cgcactggca
    10861 ggatatgtta aaactggaag cagcggcgca acgatttggc gatgggcatc tcaatgagcg
    10921 tatccacttt gatgagggtt cgagctttga acgacttggt atcgcattta accagatggc
    10981 ggacaatatc aacgccttga ttgccagcaa aaaacagctt attgacggta tcgctcacga
    11041 actgcgaaca ccgttagtgc gcctgcgtta tcgactggag atgagcgata acctgagcgc
    11101 cgccgaatcc caggcgttga atcgtgatat cagtcaactt gaagctttaa ttgaagagct
    11161 gctgacttat gcccgactcg atcgcccaca aaacgagctt catcttagcg aaccagacct
    11221 gccgttgtgg ctgtcaacgc atctggcaga tattcaggca gtaacgcccg ataaaacggt
    11281 acggataaaa acgctcgtgc aaggccatta tgcggcgttg gatatgcgct taatggagcg
    11341 cgtgctggat aatttgctca ataacgccct gcggtactgc cattcaacgg ttgaaaccag
    11401 cctgctactg tcggggaata gagcgacatt aattgttgag gatgatggcc cagggattgc
    11461 cccagaaaac cgcgaacata tctttgaacc ttttgttcgc ctcgatccca gccgggatcg
    11521 ctcaaccggc ggctgcgggc tggggctggc aattgtccac tctatagcac tggcaatggg
    11581 cggtacggtt aattgtgaca ccagcgaact gggtggtgct cgcttctcgt ttagctggcc
    11641 gttatggcat aacatcccgc aatttacctc tgcctgacac tacgcgcacg atggtcaagt
    11701 caccacgact gtgctataaa ataagtatgt tgtaactaaa gtggttaata ttatggcgcg
    11761 ttacgatctc gtagaccgac tcaacactac ctttcgccag atggaacaag agctggctac
    11821 atttgccgct catcttgagc aacacaagct attggttgcc cgcgtgttct ctttgccgga
    11881 ggtaaaaaaa gaggatgagc ataatccgct taatcgtatt gaggtaaaac aacatctcgg
    11941 caacgacgcg cagtcgctgg cgttgcgtca tttccgccat ttatttattc aacaacagtc
    12001 cgaaaatcgc agcagcaagg ccgctgtccg tctgcctggc gtgttgtgtt accaggtcga
    12061 taacctttcg caagcagcgt tggtcagtca tattcagcac atcaataaac tcaagaccac
    12121 gttcgagcat atcgtcacgg ttgaatcaga actccccacc gcggcacgtt ttgaatgggt
    12181 gcatcgtcat ttgccggggc tgatcaccct taatgcttac cgcacgctca ccgttctgca
    12241 cgaccccgcc actttacgct ttggttgggc taataaacat atcattaaga atttgcatcg
    12301 tgatgaagtc ctggcacagc tggaaaaaag cctgaaatca ccacgcagtg tcgcaccgtg
    12361 gacgcgcgag gagtggcaaa gaaaactgga gcgagagtat caggatatcg ctgccctgcc
    12421 acagaacgcg aagttaaaaa tcaaacgtcc ggtgaaggtg cagccgattg cccgcgtctg
    12481 gtacaaagga gatcaaaaac aagtccaaca cgcctgccct acaccactga ttgcactgat
    12541 taatcgggat aatggcgcgg gcgtgccgga cgttggtgag ttgttaaatt acgatgccga
    12601 caatgtgcag caccgttata aaccacaggc gcagccgctt cgtttgatca ttccacggct
    12661 gcacctgtat gttgcagatt aacgcccggc tttcatactg ccgaccatct gttctggccg
    12721 tacccagctg tcaaactcgg cttcgctaag ataccccagc gcaagggccg cagcttttaa
    12781 ggtcagccct tctttatgcg cttttttggc gatctccgcg gctttgtcat aaccaatgtg
    12841 ggtgttaagc gcagtcacca gcatcagcga ttcattgagt aattgattga ttcgctcacg
    12901 attcggttca atacccactg cgcagtgttt gttaaaactt tccatgccat ctgccagcaa
    12961 gcgcaccgat tgcaggaaat tgtggatcac cattggacgg aagacgttca gttcaaagtt
    13021 accggaagcg ccccccatgt tgatcgccac gtcgttcccc atcacctgac agcagagcat
    13081 ggttaatgcc tcacactgtg ttggattcac tttccccggc atgattgagc tgcccggctc
    13141 attttccggg attgagattt caccaattcc gcagcgcggg ccagaggcca gccagcggac
    13201 atcattggcg attttcatca gtgacacagc caaccctttc aacgcgccgt gcgcctgaac
    13261 cagggcatca caggtcgcca gcgcttcaaa tttgttcggc gcggtaacaa acggtgcaca
    13321 ggtaatgact gccagttcat ctgctacgcg acgcgcatac tccggatggg tatttagtcc
    13381 agtacccacc gctgtaccgc caagagccag ttccgctacg tgaggcaggc tgtattcgat
    13441 atgtttgaga ttatgctcga gcatcgctac ccagccggaa atctcctgcc ctagtgttag
    13501 cggcgtggcg tcctgcaagt gggttcgacc gattttgacg atatcggcaa atgcacgcga
    13561 tttttcactc agtgtctgtg tcagggtttt aagctgcgga atgagttgct tgcgcagcgc
    13621 cagcagcgcc gcaacgtgca tcgccgtcgg aaagacatcg ttggaacttt ggcttttgtt
    13681 cacgtcgtcg ttagggtgaa ctttacgttc catcccgcgc acgccgccga gtaattcact
    13741 ggcccggtta gccagcactt cgttcatgtt catgttactt tgcgtgccgg agccggtctg
    13801 ccagatagcc agcgggaatt cgtcgtcatg ctgtcctgcc agtacttcat ccgccgcctg
    13861 ccgaatggcg ctcgctttct cttcagacaa caagcctaaa tcttcattaa cttttgccgc
    13921 tgcgcgcttg gttagcgcca gcgcatgaat cagtgaggtg ggcattttct ccgtcgaaat
    13981 gcggaaatgc tccagcgagc gttgagtttg tgcgccccac agcttatctg ccgggacatc
    14041 aatcgccccc atcgaatctt tttcgctgcg tactgtattc atggcctgct cctcacctgt
    14101 ttaatttttt ctttctgttt tgctttcgtt aagcaacttt tagctcactt attatttact
    14161 atttgataac aaatgtttgg tctttcgtgc catgtaaaaa aaccgccccg aagggcggct
    14221 ctgtttattt cacacagcga gtgcactgtg tgagttgtat ctgctggaag aagtcatttc
    14281 ctttatcatc cacaaggata aacgccggga aatcttccac ttcaattttc cagatggctt
    14341 ccattcccag ttccggatat tcaacacatt ccaggctctt aatacttccc tgcgccaata
    14401 cagcggccgg accaccgata ctgccaaggt agaagccgcc gtgtttttta caggcatccg
    14461 tcacctgctg gctgcggttg cctttcgcca gcatgatcat acttccgccc tgcgcttgca
    14521 gttgatcgac ataagaatcc atccgtccgg cggtcgttgg gccaagagaa ccggaggcat
    14581 aaccttccgg cgttttggcc ggacccgcgt agtaaatcgg atgatcttta atgtactgcg
    14641 gcagcccttc accgttatcc atccgctctt tcagtttggc gtgagcaata tcacgaccga
    14701 cgataatcgt gccgttaagc gataagcgtg tagaaacggg atactgcgac aactgtgcga
    14761 ggatctcttt catcggacgg ttaaggtcaa cgcgcaccgc ttcgccttct cccgctttac
    14821 gcagctcttc agggatatat ttacctggat tatgttccag tttttcgatc cagatcccct
    14881 gacggttgat cttcgctttg atattacggt cagcagagca ggaaacaccc ataccaaccg
    14941 gacaggatgc gccgtgacgt ggcaggcgaa tcacgcgtat gtcgtgagcg aagtatttac
    15001 caccaaactg cgcacccaga ccgagatttt gcgcttcgat cagcaattct ttttccagtt
    15061 ccacatcgcg gaatgcctga ccgtgctcat tcccttccgt tggcagttcg tcatagtatt
    15121 tagcggaagc cagtttcact gttttaaggt tagtttctgc agaagttcca ccaattacga
    15181 acgcaatatg atacggagga caggccgccg tacccagcgt gcgcatcttc tcaaccaggt
    15241 aatttttcag tttccccggc gttagtaacg ctttggtttc ctgatagaga tacgtcttgt
    15301 ttgccgaacc accacctttg gcgatacaga ggaatttgta ctcgtcgcca tcaacggcat
    15361 aaagatcgat ctgcgctggc agattggtgc cggtattcac ctctttatac atatccagcg
    15421 gcgcgttttg cgagtagcgc aaattatctt cgatataagt gttatagaca ccgcgcgcca
    15481 gcgccgcttc atcacagcca ccggtccata cacgctgccc ttttttacca acaataatcg
    15541 cagtgccggt atcctgacag gttggcagaa cgcctttcgc cgcgatgtcg gagttacgca
    15601 ggaattgcag cgccacatat ttatcatttt cgctggcctc cgggtcacgc agaatgtcgg
    15661 ccacctgttg ttggtgagcc ggacgcagca taaacgacgc atcatgaaac gcctgacgcg
    15721 ccaacagagt taacgcttcg ggtgcgactt tcaaaatctc ctgcccttca aattcagata
    15781 cgctaacgtg ttcgctggtt agcaggtaat actcagtatc atcttttttg agtggaaaag
    15841 gagcctgata atgaaagggt ttgtttgaca ttgttctctc acttactgcc tggtttggtt
    15901 atgctctggg cgggtgttcc gttgccctgt taaaagcgag taacaatatc ctacacactt
    15961 ttttaacaaa aactgagact agtacgactt tttgcggctc caggttactt cccgtaggat
    16021 tcttgcttta atagtgggat taatttccac attaaaacag ggattgatca tgcaaaaact
    16081 cattaactca gtgcaaaact atgcctgggg cagcaaaacg gcgttgactg aactttatgg
    16141 tatggaaaat ccgtccagcc agccgatggc cgagctgtgg atgggcgcac atccgaaaag
    16201 cagttcacga gtgcagaatg ccgccggaga tatcgtttca ctgcgtgatg tgattgagag
    16261 tgataaatcg actctgctcg gagaggccgt tgccaaacgc tttggcgaac tgcctttcct
    16321 gttcaaagta ttatgcgcag cacagccgct ctccattcag gttcatccaa acaaacgcaa
    16381 ttctgaaatc ggttttgcca aagaaaatgc cgcaggtatc ccgatggatg ccgccgagcg
    16441 taactataaa gatcctaacc acaagccgga gctggttttt gcgctgacgc ctttccttgc
    16501 gatgaacgcg tttcgtgagt tttccgagat tgtctccata ctccagccag tcgcaggtgc
    16561 acatccggca attgctcact ttttacaaca gcctaatgcc gaacgtttaa gcgaactgtt
    16621 cgccagcctg ttgaatatgc agggtgaaga aaaatcccgc gcgctggcga ttttaaaatc
    16681 ggcgctcgac agtcagcagg gtgaaccgtg gcaaacgatt cgtttaattt ctgaatttta
    16741 cccggaagac agcggcctgt tctccccgct gttactgaat gtggtgaaat tgaatcctgg
    16801 cgaagcgatg ttcctgttcg ctgaaacacc gcacgcttac ctgcaaggcg tggcgctgga
    16861 agtgatggcg aactccgata acgtgctgcg tgcgggtctg acgcctaagt acattgatat
    16921 tccggaactg gttgccaatg tgaaattcga agccaaaccg gctaaccagt tgttgaccca
    16981 gccggtgaaa caaggtgcag aactggactt cccgattcca gtggatgatt ttgccttctc
    17041 gctgcatgac cttagtgata aagaaaccac cattagccag cagagtgccg ccattttgtt
    17101 ctgcgtcgaa ggcgatgcaa cgttgtggaa aggttctcag cagttacagc ttaaaccggg
    17161 tgaatcagcg tttattgccg ccaacgaatc accggtgact gtcaaaggcc acggccgttt
    17221 agcgcgtgtt tacaacaagc tgtaagagct tactgaaaaa attaacatct cttgctaagc
    17281 ttattaaagg cttataacac cttcaggcgg ccagtccgcc tgatttcatt ttatggataa
    17341 tcattatgaa taaatcgctg gtagcggtag gcgtcattgt tgcgctaggc gtagtctgga
    17401 caggcggcgc atggtataca ggcaagaaga ttgaaaccca tctcgaagac atggtcgcgc
    17461 aggcgaacgc gcaactcaaa ctgacagctc ctgaatccaa cctggaagtg agttatcaaa
    17521 actatcatcg cggcgtattc agcagccagt tgcaactgtt ggtgaaaccc attgccggga
    17581 aagaaaatcc gtggattaaa agcggtcaga gcgtcatctt caacgaatcg gttgatcatg
    17641 gtcccttccc gcttgcccag cttaaaaaac tgaacctgat cccgtcgatg gcatcaattc
    17701 aaaccacgct ggttaataac gaagtaagca aaccactgtt tgatatggca aaaggtgaaa
    17761 cgccttttga gattaactcg cgcattggtt acagcggtga ttccagttcc gatatttcgc
    17821 tcaagccact gaattacgag caaaaggatg aaaaagtcgc ctttagcggc ggcgagttcc
    17881 agttaaatgc tgacagagac ggcaaagcca tctccctttc cggggaggcg caaagtggtc
    17941 ggatagacgc agttaacgaa tacaaccaga aagtgcagtt gacctttaat aatctgaaaa
    18001 ccgacggttc cagcacgctg gcaagttttg gtgagcgcgt aggaaaccaa aaactgtcac
    18061 tggaaaaaat gaccatttca gtggaaggca aagaactggc actgctggaa ggcatggaga
    18121 tcagcggtaa atcggatctg gtcaatgacg gtaaaacgat caatagccaa ctggattact
    18181 cgctaaacag cctgaaggta cagaatcagg atctgggcag cggcaagctg actttaaaag
    18241 tcggccagat tgatggtgaa gcctggcatc agtttagcca gcaatataac gcgcaaactc
    18301 aggcgctgct ggcacagcca gaaattgcca acaatcccga actttatcag gagaaagtga
    18361 cggaagcctt ctttagcgcc ctgccgctga tgttgaaagg cgatccggtg attactatcg
    18421 cgccgctaag ctggaaaaac agtcagggtg aaagtgcgct gaatctgtcg ctgttcctga
    18481 aagatccggc aacgactaaa gaagcgccgc aaacgctggc gcaggaagta gatcgttcgg
    18541 ttaaatctct ggatgcgaaa ctgaccattc cggtggatat ggcaactgag tttatgactc
    18601 aggtagcgaa gctggaaggt tatcaggaag atcaagcgaa aaaactggcg aaacagcaag
    18661 ttgaaggtgc atcagcaatg gggcagatgt tccgtctgac caccttgcag gacaatacca
    18721 tcaccaccag cctgcaatat actaacggtc agataacgtt aaacgggcag aaaatgccac
    18781 tggaagattt cgttggtatg tttgcaatgc cggcattaaa tgttccggtc gtacccgcta
    18841 ttccgcagca gtaattcaca gacaaaacgc cggagatttt tctctccggc gttatttttt
    18901 acttcagcat aaagtcatac tgaatactca ttttcacatc ccagtaattc aggcgatcgc
    18961 ccagcgtact gccatcgcgc ttttcccgcc ctttatcggc agcaagaaag aaattcatcc
    19021 cttgtaacgg cccttcgctg acccgatagt tcataccacc taagataccc gtgccttcaa
    19081 tccgcacatc tcgtttactg gattcatagc cgcgagtcac aatcggtgca aatgtcagcg
    19141 taaattgcgg cgttaaacga tagttgacgc ccaattgcca ggactgcatt ccttcacggt
    19201 tacgatcaag acttaatgaa aagggatagc caatgtcggt atccaccacg gcaccattca
    19261 ttgcatggcg caaataacca ccactgccaa atacgcttcc ccacggcgca ttccaggtca
    19321 attgtccact aaccaacgcc gtttcattgg gctggctggt attacgactt aaaccttcga
    19381 gctgcgcgta aaagccgagc aactcccctt tcaacgtata attcgggtcg agcggtatgc
    19441 ttttctcgag aatactttgt atctgattgg aaacatcttt ctgtaccgat gcccccagag
    19501 aatagcgact ggtatcatca tgccaactgg cggctaaaaa agaacgtgcg cgatgtttat
    19561 cgtattttcc cgtctggctg ttatacaccc cttcatcacg aataccggtt gttgaatcag
    19621 tccataaaaa tactcgtgaa cgccacattc cccacagatc aatatttggt gttaaagccc
    19681 aataacctaa tgctccttca gaaactgtcg gtaatgtacg ttgcgaactg ccagccatta
    19741 acggaatacg ataaggtaag ctaccataac tataatccgt cccgaagcga cctagtttaa
    19801 gcaggctatt ttcactgggt tttattttta ctgcccccag ggcaaagcca aaacttttat
    19861 caccatcaag ataccaccgg gtactcatat cagcacgagc acctaattta taaacataat
    19921 atgccccgcc ttcaacgcca ataatattgg aataataacc actattgaaa tcgagcaatc
    19981 ctccctgtac ccatgcgtaa atatctcttc ccgcgccagc actgggctta tccgccctgc
    20041 gcaattcgtt tttcagtctg atgcgtagtc ccgcttcatc gtcagccagt tgcgccgcat
    20101 aagcagaggt aaggccagag gcagccgtca ggcaaataac ggccatggcc actatttttc
    20161 tcatagttgc acctttgatg ttctgattat ttttattgaa tattaattag tgatatcgct
    20221 gattaattgc tgctgcactt ttttacgatt atcaatttca accacgattt ctttgaattt
    20281 tttatccgtg agcggataaa accagataat aacgaatgcc agtagcataa atccgcaagg
    20341 tactaaggca attgatgtgc ggatgcccat aataacttcc ggcgtttgca cctgattggc
    20401 gatatatccg cttaatccca aaataaaggc aggaattgaa cctccgattg cctgaccgca
    20461 tttacgggta aatgagaata gtgaataggt gagcccttca attcgcacgc cggtcaggta
    20521 ttcaccgtat tctacggtat cagcttccag tgcccacatc acagtcatag taacgccctg
    20581 accaattgaa gcgatggcca acgcaaccaa cgccaccggc agtgaccaga cggaaaccca
    20641 gaagaatagc agataaccgc aggttcccag caaagcgcca atcaggaagg tattcttttt
    20701 accgatcctc gcgaccatcc ccggcaccag cggtgccgat gccacagtac caaccaggtt
    20761 ttgcaccagt accagcacag tgaacagccc ggtatcattt aacacatagc gcacgtagaa
    20821 caacgacgag gcgctgaccg cgaaggtcga aatcagcaca cacagcgcac cgatgcacaa
    20881 cataaacagc gggcgattcc gtttcagggt ttgcagactg atcttcaatg acggctgcgc
    20941 cacgatacgt accacattct cacgcgtcga tttgaagcag atgaagtaaa gcaccattcc
    21001 ggcaatcgcc agcacaatcg tccagaaatg gtataccgac accatctctt ccgggctgga
    21061 gttcttaatg ctcggtccta tcagaaatgc caggcagaca aaggttaatg aagcggcaat
    21121 cccgcgagcc gcgcccagac gggcgcggga ttgtggttgt tgggtcatcg cggtagcaag
    21181 tgaaccataa ggaatattca ccaggctgta gcaaagcccg aggcccatgt aggtcaaata
    21241 tgcatacacc actttgctac catggctcca gtcggtcggc acccagaata ccagcacgct
    21301 gaagatcatt aacggcgcag taccgaagag taaaaacggg cggaattttc cccagcgggt
    21361 attcacactg tccaccactc gtccggcaaa gacgtcggcg aaggcatcga atacccgcac
    21421 cagtaacagc atggtgcccg ccgcagcggc accgacgcca gcgacgtcgg tgtagtaact
    21481 caataggaag agcgccccca ttgcgaaggc gaagttattg gcgacgtcac cgaggctgta
    21541 gccgacgatg gtgcgccagg agagttgttg attcattgtt tgcctccctg ctgcggtttt
    21601 tcaccgaaat tcatgccagt ccagcgtttt tgcagcagaa aagccgccga cttcggtttg
    21661 cggtcgcgag tgaagatccc tttcttgtta ccgccaacgc gcaatatgcc ttgcgaggtc
    21721 gcaaaatcgg cgaaattcca tacctgttca ccgacgacgg cgctgacgcg atcaaagacg
    21781 cggtgataca tatccagcca tgcacactga tactcttcac tccacatgtc ggtgtacatt
    21841 gagtgcagcc cggctaacgt atccacgccg tattcggtga tgataatcgg ctgatgcagt
    21901 ttctcctgcc aggccagaag ttctttttcc agtaccttct ctgccgtttc caaatcgccg
    21961 ctttggacat accatccgta ataacggttc aggcacagca catcaaagag atcgctgatg
    22021 gtatcggtgt gagcgtcgca gaacattaca ttgacgcagg tgatcggacg cgtcgggtcg
    22081 agtttacgcg ttgcttccgc cagtggcgcg aaatattccc gtgcaccttg cggacgggta
    22141 tccggttcgt tggcaatact ccacatcacc acgcttgggt ggtttttgtc acgcgctatc
    22201 agctctttaa tcgcctgtaa gtgcgcttgc tgagtttccc cgttgactgc ctcttcgctg
    22261 tacagttctt tcggcttgtt gcccgcttcg aaaccaatgc ctaaagagag gttaaagccg
    22321 acagcagcag tttcatcaat caccacgatg ccatgttcat ctgcccagtc gagcatctct
    22381 tcagcgtaag ggtaatgcga ggtacggtag gagttggccc caatccagtc cattaatgcg
    22441 tggtcgtgca ccatcagcac gttatcgaat cctttgccac gcaagtccgc atcttcatga
    22501 cgaccaaagc cagtaaagta gaacggtttg tggttaatca ggaactgttc gcccttcact
    22561 gccactgacc ggatgccgac gcgaagcggg tagatatcac actctgtctg gcttttggct
    22621 gtgacgcaca gttcatagag ataaccttca cccggttgcc agaggtgcgg attcaccact
    22681 tgcaaagtcc cgctagtgcc ttgtccagtt gcaaccacct gttgctccgc atcacgcagt
    22741 tcaacgctga catcaccatt ggccaccacc tgccagtcaa cagacgcgtg gttacagtct
    22801 tgcgcgacat gcgtcaccac ggtgatatcg tccacccagg tgttcggcgt ggtgtagagc
    22861 attacgctgc gatggatccc ggcatagtta aagaaatcat ggaagtaaga ctgctttttc
    22921 ttgccgtttt cgtcggtaat caccattccc ggcgggatag tctgccagtt cagttcgttg
    22981 ttcacacaaa cggtgatacg tacacttttc ccggcaataa catacggcgt gacatcggct
    23041 tcaaatggcg tatagccgcc ctgatgctcc atcacttcct gattattgac ccacactttg
    23101 ccgtaatgag tgaccgcatc gaaacgcagc acgatacgct ggcctgccca acctttcggt
    23161 ataaagactt cgcgctgata ccagacgttg cccgcataat tacgaatatc tgcatcggcg
    23221 aactgatcgt taaaactgcc tggcacagca attgcccggc tttcttgtaa cgcgctttcc
    23281 caccaacgct gatcaattcc acagttttcg cgatccagac tgaatgccca caggccgtcg
    23341 agttttttga tttcacgggt tggggtttct acaggacgta acataaggga ctcctcatta
    23401 agataaaaat actggtcaac ctttaatctg attagttaac caattctgtg ttgcacacag
    23461 ttatctgtga gaaacccatg gaagagaaat ggaaataaaa agaactgaca ccgcgatcaa
    23521 aaaaccaaaa actgcaatac ccacagcgtg atattgatcg cattaatctt taaaacggcg
    23581 ttgacagaag ccataaaata agcaaccgaa cgttcgttcg agtatattgg gaacgataaa
    23641 ctctcatgac atatggctac ggtgaatatt tttggggggg aattctccga tcccggctag
    23701 attgtatttt gtacagggaa attaaagtat gaaacgcggg cacagttatc accagcccgc
    23761 gaaatgagag gatcaggatg cggttaagat accgccaatc atcgcgcgta atccctgagc
    23821 aaacgacgct tcacgcgcca gatcttccgc ttcgatatcg gccagtacac cgtaggtcag
    23881 atccagcagt aaacgtgaag tcattgacgt gttgatgtct gggcttatct cgcctttttg
    23941 ctgggcatca cgcatccgct cggcaacaaa ttcagtgatc gtcatatgct tatttttaag
    24001 catggcggcg acctgtgggt tacgcatccc ttccgccata atttcgacca ccagcgcccg
    24061 ttgcccaaag gcttcatggg tgagagagac aatagactcg accatatagt caacaaaatg
    24121 aatcccttca atcggttccc ggaaacgggc cagcgccctc tcctggtcct gtaagataat
    24181 cgcctgaatc aaggcttctt tggagatgaa atggtgatag agcgtcccgg gactaatggc
    24241 gcaggattta cagatggctt tcatcgaggc actgtgaaat ccattttctg aaaaaatctc
    24301 tctggcagca ttgaggatcc gggtccgtgt cggttgtgct tcagtctgca tgttatccat
    24361 cataaaacca ttatagttag cgtgaataac tagtttaccg cgttgtcagg tgagcgccta
    24421 tcattgctgt tgaatagtgc gaaggcacac tctattcgta tacttttcac tctgaaattg
    24481 gttaaccaat agccgtgatt tggctgccag ttatttagca gagacgttga tgagtttatc
    24541 ccggtaaaca acacgcacgg ttgtttaccg gtccgttagt gtattaattg agctcctgta
    24601 ccccaccacc ggagacggtg agaatttgtc cgcttaccca gctcgcagca ggcgagcaaa
    24661 ggaacagcgc tgcgttagca atatcttgcg gttggcccag acgtctgatc ggcgtgtgct
    24721 gtaacatttt ttgttcaatt tctggtgtaa taacggattt cagggcatcg gttaatattg
    24781 cccccggcgc aatgccattt acccgaatat ttttttcacc caggtcaaac gccatatttc
    24841 tgaccagatg actggctgca gctttagatg atgcatagga agtcatgttt atatttttat
    24901 tttctgccgc catagaagtg atggtcagaa taacgccacc gccatttttt tccatttctg
    24961 gcgcaacaag ttgtgacaga tggaaaaaag aaaacacatt cagttcataa gcacggcgaa
    25021 aatccgccat tggcatatca aacggtttag gtccaccgcc accggcgttg ttaaccagaa
    25081 tatcaacttt acccagctta ctgatagcaa agtctgccag tgcagagagt tcctgttcgg
    25141 aagtaatatc acaacggcag gcaaatgcct gaccacccag ttgttgaatt tcgtctacaa
    25201 catggttagc tgcgtcggcg ttaatatcac tgaccaccac agatgcgcca gctgtcgcga
    25261 atgtaatggc gatttcttta ccaatacctg cacccgcacc tgtgatgatg gcgcattttc
    25321 cgtcgagtct caggttgtca gaattaaaca cataaacctc ctgtgaactt cagtaaaaat
    25381 aaaaggcacc gcctgtgtgc cacatttttt atcattaacc ttgaggaaag gatactacca
    25441 ggtatagcag ataaagagag gattatttcg ccgcaatgag ccgggcggga ataataagat
    25501 tgcgtgaatg cgtctcttca tgggtgattt tttgcatcat gcgatccgca agtgtgatac
    25561 caagttcccg cgctggcgtg ctggcccagg taacaggtat atcatcaagt gtggttggtg
    25621 tcgcatcggt aaatgccgcc agcgaaacct gttgctcaaa gtaacgatcg actccgcttt
    25681 ccccgctttg ccgccctgct ttgagcaaac caaaccatgc ccccatcgca atagtttcgt
    25741 tatagcaaac cacggcactg atggtcgggt tatgacgtaa aagcgccgtg atagcttccg
    25801 cggcttgctt ctggctggaa gtgcactcca acacccaatc gctatgaaac ggcaggccaa
    25861 attttagtag agttgcacaa tagcccccca cccgttctgc acgggttaat gaggaacttt
    25921 gccctcccag ccaggcgatc cgctgatgcc cattgcgaat gagatgctcc gtcaacaact
    25981 gtgcagcctg catgttgtcc gggcgaaccg tatcaacatc atcaagataa ctggcacggg
    26041 aagcgaaaat caccgggata gctttttctt ctgccatccg tcgcaggtca tcgctgcttc
    26101 ctgcagcccc ggcaattacc acaccatcca caccctgatt cagtaacagt gaaaaccgct
    26161 gtgccagctg ctcgccgtct ttaccgccgt gaagcaaaaa aaccatccgt ccctgcgctt
    26221 ccagagcttc cgtcaatccg gccgtcaatt cggcgtaaaa cggcgcagat aaatcacgga
    26281 cgatcaaacc aatgacgccg ctttgcccgc cgcgcagcgc cgacgcctgg cgattgcgca
    26341 caaatcccag ctcttcaatg gcggcgttaa cgcgttctcc tgtggcggta gagattcgcc
    26401 ctttgccact aagcaccagc gaaacggtac ttaccgacac gcccgcagcc agcgcaacat
    26461 catgaatggt tatttttttg gcggtagcca tgcagatgac ctactccctg attatgtgac
    26521 agataaaacg ttttaccttt tatttatctt atactcgcta ttatcgttgc gtaatgtgat
    26581 ttatgcctca ctaaaatttg ataaaacgtt ttatcttctc gcgcaattta ctgaatccag
    26641 attgttctct acgaggagtc gttttatgac ggcgaaaaca gcaccgaaag tcacgctgtg
    26701 ggagttcttc cagcagttag gcaaaacctt catgttaccc gtggcattat tgtcgttctg
    26761 cggcattatg ctcggcattg gtagttctct tagtagccac gatgtcatca ccctgatccc
    26821 ggtcctgggc aaccccgtgt tgcaggctat ctttacctgg atgagtaaga ttggctcgtt
    26881 tgcttttagt ttcctgccag tcatgttctg tatcgccatc ccgctgggtc tggcacgcga
    26941 aaacaaaggc gtagcggcat tcgctggctt cgtcggttat gcggtaatga acctcgcggt
    27001 aaacttctgg ttgaccaata aaggcattct gccaaccacg gacgccgcgg ttctgaaagc
    27061 caataacatc cagagcattc ttgggatcca gtcgatcgac accggtatcc tcggtgcggt
    27121 gatcgccggt attatcgtct ggatgctgca tgagcgattc cataatatcc gcctgccgga
    27181 tgcgcttgca ttcttcggcg gtacgcgctt cgtaccaatc atctcctcgc tggtgatggg
    27241 ccttgtcggc ctggtgattc cattagtctg gccgattttc gcgatgggta ttagcggctt
    27301 gggccatatg ataaacagcg cgggtgattt cggaccgatg ctgtttggta ccggtgaacg
    27361 tctgctgttg ccgtttggtc tgcatcacat tctggtggca ttaattcgct ttaccgacgc
    27421 aggcggcacg caggaagtct gcggtcaaac cgtcagcggc gcattgacca tcttccaggc
    27481 gcaattgagt tgcccgacca ctcacggttt ttctgaaagc gccacgcgtt tcctttcgca
    27541 aggtaaaatg cctgcgtttc tcggcggtct gccaggtgca gcgttagcta tgtatcactg
    27601 cgcgcgcccg gaaaatcgcc ataaaattaa aggtctgctg atttctggcc tgatcgcctg
    27661 cgtcgttggc ggcactaccg aaccgctgga attcctgttc ctgttcgtag cgccagttct
    27721 gtatgtcatc cacgcgctgt taaccggcct cggcttcacc gtcatgtctg tgctcggcgt
    27781 caccatcggt aataccgacg gcaatatcat cgacttcgtg gtgttcggta ttttgcatgg
    27841 tctgtcaacc aagtggtaca tggtgccagt ggtggcggca atctggtttg tcgtttacta
    27901 cgtcatcttc cgtttcgcta tcacccgctt caatctgaaa accccggggc gcgatagcga
    27961 agttgccagc tcaatcgaaa aagccgttgc cggtgcgccg ggtaaatcag gttacaacgt
    28021 tcctgcaatc ctcgaagcat taggcggtgc cgacaatatt gtcagcctcg ataactgcat
    28081 tacccgtctg cgtttgtctg tgaaagatat gtcgcttgtt aatgtgctgg cactgaagga
    28141 caatcgggca attggcgtag tacaacttaa tcaacataac ctgcaggttg ttatcgggcc
    28201 acaagttcag tcagtaaaag atgaaatggc cggtctgatg catactgtcc aggcataagg
    28261 ataagatatg ttcgattttt caaaggtcgt ggatcgtcat ggcacatggt gtacacagtg
    28321 ggattatgtc gctgaccgtt tcggcactgc tgacctgtta ccgttcacga tttcagacat
    28381 ggattttgcc actgccccct gcattatcga ggcgctgaat cagcgcctga tgcacggcgt
    28441 atttggctac agccgctgga aaaacgatga gtttctcgcg gctattgccc actggttttc
    28501 cacccagcat tacaccgcca tcgattctca gacggtggtg tatggccctt ctgtcatcta
    28561 tatggtttca gaactgattc gtcagtggtc tgaaacaggt gaaggcgtgg tgatccacac
    28621 acccgcctat gacgcatttt acaaggccat tgaaggtaac cagcgcacag taatgcccgt
    28681 tgctttagag aagcaggctg atggttggtt ttgcgatatg ggcaagttgg aagccgtgtt
    28741 ggcgaaacca gaatgtaaaa ttatgctcct gtgtagccca cagaatccta ccgggaaagt
    28801 gtggacgtgc gatgagctgg agatcatggc tgacctatgc gagcgtcatg gtgtgcgggt
    28861 tatttccgat gaaatccata tggatatggt ctggggcgag cagccgcata ttccctggag
    28921 taatgtggcg cggggagact gggcgttgct gacgtcgggc tcgaaaagtt tcaatattcc
    28981 cgcgctgacc ggtgcttacg ggattataga aaatagcagc agccgtgatg cctatttatc
    29041 ggcactgaaa ggacgtgatg ggctttcttc cccttcggta ctggcgttaa ctgcccatat
    29101 cgccgcctat cagcaaggtg caccgtggct ggatgcctta cgcgtctatc tgaaagataa
    29161 cctgacgtat atctcagata aaatgaacgc cgcgtttcct gaactcaact ggcagatccc
    29221 gcaatccact tatctggcct ggcttgatct acgtctgttg aatattgacg acaacgcgtt
    29281 gcaaaaagcg cttatcgagc aagaaaaagt cgcgatcatg ccggggtata cctatggtga
    29341 agaaggtcgt ggttttgtcc gtctcaatgc cggctgccca cgttcgaaac tggaaaaagg
    29401 tgtggctgga ttaataaacg ccatccgcgc tgttcgctaa ccccaattgc gcaacgtaaa
    29461 aaatcgttgc gcaatcgtgg atttttaccc tgctttgttt ttataatggt gcgcactttt
    29521 atattcagaa aaagagtgcg accatgattg ataccaccct gccattaact gatatccatc
    29581 gccaccttga tggcaacatt cgtccccaga ccattcttga acttggccgc cagtataata
    29641 tctcgcttcc tgcacaatcc ctggaaacac tgattcccca cgttcaggtc attgccaacg
    29701 aacccgatct ggtgagcttt ctgaccaaac ttgactgggg cgttaaagtt ctcgcctctc
    29761 ttgatgcctg tcgccgcgtg gcatttgaaa acattgaaga tgcagcccgt cacggcctgc
    29821 actatgtcga gctgcgtttt tcaccaggct acatggcaat ggcacatcag ctgcctgtag
    29881 cgggtgttgt cgaagcggtg atcgatggcg tacgtgaagg ttgccgcacc tttggtgtgc
    29941 aggcgaagct tatcggcatt atgagccgga ccttcggcga agccgcctgt cagcaagagc
    30001 tggaggcctt tttagcccac cgtgaccaga ttaccgcact tgatttagcc ggtgatgaac
    30061 ttggtttccc gggaagtctg ttcctttctc acttcaaccg cgcgcgtgat gcgggctggc
    30121 atattaccgt ccatgcaggc gaagctgccg ggccggaaag catctggcag gcgattcgtg
    30181 aactgggtgc ggagcgtatt ggacatggcg taaaagccat tgaagatcgg gcgctgatgg
    30241 attttctcgc cgagcaacaa attggtattg aatcctgtct gacctccaat attcagacca
    30301 gcaccgtagc agagctggct gcacatccgc tgaaaacgtt ccttgagcat ggcattcgtg
    30361 ccagcattaa cactgacgat cccggcgtac agggagtgga tatcattcac gaatataccg
    30421 ttgccgcgcc agctgctggg ttatcccgcg agcaaatccg ccaggcacag attaatggtc
    30481 tggaaatggc tttcctcagc gctgaggaaa aacgcgcact gcgagaaaaa gtcgccgcga
    30541 agtaacaaaa tggatggtgc aaattgcacc atccattttt catgcaaggc acaaagtcgc
    30601 gcgatgtttg gcggattcga tgcccagctc aatcaactcc attacctgga ttgcctggct
    30661 tgccggaacc ggattttcac catcgccatt taacgcatca cgaatagccg catagtaagc
    30721 cggataattc ccaggtaccg tcaacagcgt ttcttcgaca cgttcctcac cttccacgcg
    30781 ggtaagtacg ccatcacgca tatcgtagcc ccagtcttcc tgcggcagac gctcgccatt
    30841 tttcagacgt tcttcctgtg gatcgaggcc atatttcaca taactgcctc gggatccatg
    30901 cacgatatac cgtgctgact cagcggctgc cagcatggta ccgtgtaaaa tgactcgccg
    30961 ctgtggatag gacaagatgg cgtggaaata atcggtcgac tgcgctccgg gccgtaactg
    31021 tgccaaatct accgtcatgc tgaccggtaa accaaaaagc gtaatggcct gatcaagaag
    31081 atgtggtgct aaatcgtacc agataccgct gcctgggccg ccctgttcac gccaacgatc
    31141 gcgcacctgc ggacggaagc ggtcaaaatg agactcaaag taagcaactt cacccagcac
    31201 gccttccgcg agtaaacctt ttagcgtcaa gaaatcgcta tcccagcgac ggttatggaa
    31261 tacagacagc acacgcccca ggctttttgc cagcgcatcc agctctcgcg cttgtgacag
    31321 tgtcacggta aagggtttat caacgaccac atgtttaccc gcctcaagcg ccgctttggc
    31381 taacgggaaa tgggtatcgt tgggtgtagg aatgacaatc aggtctatgt tgggatcgtt
    31441 aaacagatgc ttcggctcag agacaaccgt aaccgttggc cagtcggctt ttacttttgt
    31501 ttcatcactg ctggagatta ccgccagttc cagcccgggc gtgcccgcaa tcaggggcgc
    31561 atggaaggtt ttgctcgcat aaccataccc aatcaaccca acacggatgt tgtcgctcat
    31621 gtcattgcct ctcattgaag tatgatggct atttgacact atcctttacc cacgctcaac
    31681 agtttaataa cctgccagca ataagggatg ttgtttaact taagtcaaaa aaatagcgaa
    31741 ttttccaacg acaaaagcta aatatcgcaa aaacctcagt aaaaatcttg ctggagctat
    31801 tattgctaag taacatttac cccctgaagt taatggatca atcaagagag atgtgggctg
    31861 taatgaatcg tcttattgaa ttaacaggtt ggatcgttct tgtcgtttca gtcattcttc
    31921 ttggcgtggc gagtcacatt gacaactatc agccacctga acagagtgct tcggtacaac
    31981 acaagtaagc tctgcacttg tggagcgaca tgctgcccgt ccgggtgcat gttttcactt
    32041 gtcggatatt aaaccaggaa tttattatct tgttcgatgt tgttggtgat tgtcagggat
    32101 agtaaagcgc gttactcttt tcagagcagt ttttcatccc tgaatatttg ccatcagcgc
    32161 attcttatta ttgtaataac acaagcgttc actt
//
LOCUS       DAZGJW010000047        31440 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZGJW010000047 DAZGJW010000000
VERSION     DAZGJW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.047"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            192..1049
                     /gene="focA"
                     /locus_tag="ACHKIK_002133"
     CDS             192..1049
                     /gene="focA"
                     /locus_tag="ACHKIK_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO9083302.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1104..3386
                     /gene="pflB"
                     /locus_tag="ACHKIK_002134"
     CDS             1104..3386
                     /gene="pflB"
                     /locus_tag="ACHKIK_002134"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO9083303.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3578..4318
                     /gene="pflA"
                     /locus_tag="ACHKIK_002135"
     CDS             3578..4318
                     /gene="pflA"
                     /locus_tag="ACHKIK_002135"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO9083304.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4400..4990)
                     /gene="ycaK"
                     /locus_tag="ACHKIK_002136"
     CDS             complement(4400..4990)
                     /gene="ycaK"
                     /locus_tag="ACHKIK_002136"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFO9083305.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            5090..5998
                     /gene="ycaN"
                     /locus_tag="ACHKIK_002137"
     CDS             5090..5998
                     /gene="ycaN"
                     /locus_tag="ACHKIK_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9083306.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            complement(5999..7429)
                     /gene="ycaM"
                     /locus_tag="ACHKIK_002138"
     CDS             complement(5999..7429)
                     /gene="ycaM"
                     /locus_tag="ACHKIK_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO9083307.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPFLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTASAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(7638..8786)
                     /gene="ycaD"
                     /locus_tag="ACHKIK_002139"
     CDS             complement(7638..8786)
                     /gene="ycaD"
                     /locus_tag="ACHKIK_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9083308.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            9101..9727
                     /gene="ycaC"
                     /locus_tag="ACHKIK_002140"
     CDS             9101..9727
                     /gene="ycaC"
                     /locus_tag="ACHKIK_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO9083309.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(9763..10626)
                     /gene="dmsC"
                     /locus_tag="ACHKIK_002141"
     CDS             complement(9763..10626)
                     /gene="dmsC"
                     /locus_tag="ACHKIK_002141"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO9083310.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(10628..11245)
                     /gene="dmsB"
                     /locus_tag="ACHKIK_002142"
     CDS             complement(10628..11245)
                     /gene="dmsB"
                     /locus_tag="ACHKIK_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO9083311.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(11256..13700)
                     /gene="dmsA"
                     /locus_tag="ACHKIK_002143"
     CDS             complement(11256..13700)
                     /gene="dmsA"
                     /locus_tag="ACHKIK_002143"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO9083312.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            complement(13939..15231)
                     /gene="serS"
                     /locus_tag="ACHKIK_002144"
     CDS             complement(13939..15231)
                     /gene="serS"
                     /locus_tag="ACHKIK_002144"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO9083313.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(15322..16665)
                     /gene="rarA"
                     /locus_tag="ACHKIK_002145"
                     /gene_synonym="mgsA"
     CDS             complement(15322..16665)
                     /gene="rarA"
                     /locus_tag="ACHKIK_002145"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO9083314.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            complement(16676..17287)
                     /gene="lolA"
                     /locus_tag="ACHKIK_002146"
     CDS             complement(16676..17287)
                     /gene="lolA"
                     /locus_tag="ACHKIK_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO9083315.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            complement(17442..21548)
                     /gene="ftsK"
                     /locus_tag="ACHKIK_002147"
     CDS             complement(17442..21548)
                     /gene="ftsK"
                     /locus_tag="ACHKIK_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO9083316.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQSYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            complement(21683..22177)
                     /gene="lrp"
                     /locus_tag="ACHKIK_002148"
     CDS             complement(21683..22177)
                     /gene="lrp"
                     /locus_tag="ACHKIK_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO9083317.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            22721..23686
                     /gene="trxB"
                     /locus_tag="ACHKIK_002149"
     CDS             22721..23686
                     /gene="trxB"
                     /locus_tag="ACHKIK_002149"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO9083318.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGSGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            23809..25575
                     /gene="cydD"
                     /locus_tag="ACHKIK_002150"
     CDS             23809..25575
                     /gene="cydD"
                     /locus_tag="ACHKIK_002150"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO9083319.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            25576..27297
                     /gene="cydC"
                     /locus_tag="ACHKIK_002151"
     CDS             25576..27297
                     /gene="cydC"
                     /locus_tag="ACHKIK_002151"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO9083320.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFRLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            27339..28043
                     /gene="aat"
                     /locus_tag="ACHKIK_002152"
     CDS             27339..28043
                     /gene="aat"
                     /locus_tag="ACHKIK_002152"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO9083321.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            28328..28546
                     /gene="infA"
                     /locus_tag="ACHKIK_002153"
     CDS             28328..28546
                     /gene="infA"
                     /locus_tag="ACHKIK_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO9083322.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            28800..28887
                     /locus_tag="ACHKIK_002154"
     tRNA            28800..28887
                     /locus_tag="ACHKIK_002154"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28834..28836,aa:Ser,seq:gga)
     gene            complement(29038..29319)
                     /locus_tag="ACHKIK_002155"
                     /pseudo
     CDS             complement(29038..29319)
                     /locus_tag="ACHKIK_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(29601..30605)
                     /locus_tag="ACHKIK_002156"
     CDS             complement(29601..30605)
                     /locus_tag="ACHKIK_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HFO9083323.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(30677..31237)
                     /locus_tag="ACHKIK_002157"
                     /pseudo
     CDS             complement(30677..31237)
                     /locus_tag="ACHKIK_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(31322..>31440)
                     /locus_tag="ACHKIK_002158"
     CDS             complement(31322..>31440)
                     /locus_tag="ACHKIK_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083324.1"
                     /translation="IFDNDEDKADSAALLPCIEQAREDLHTLRQLQLLQQNR"
BASE COUNT         7691 a         8136 c         7702 g         7911 t
ORIGIN      
        1 gaaaaaattc aactcatttg ttaattttta aaatttattt ttatttggat aatcaaatat
       61 ttactccgta tttgcataaa aactatgcga gttacgggcc tataagccag gcgagatatg
      121 atctatatca atttctcatc tataatgctt tgttagtatc tcgtcgccga cttaataaag
      181 agagagttag tgtgaaagct gacaaccctt ttgatctttt acttcctgct gcaatggcca
      241 aagtggccga agaggcgggt gtctataaag caacgaaaca tccgcttaag actttctatc
      301 tggcgattac cgccggtgtt ttcatctcaa tcgcattcgt cttctatatc acagcaacca
      361 ctggcacagg cacaatgccc ttcggcatgg caaaactggt tggcggcatt tgcttctctc
      421 tggggctgat tctttgtgtt gtctgcggag ccgatctctt tacttccaca gtgttgattg
      481 ttgttgctaa ggcgagtggg cgcatcacct ggggtcagtt ggcgaaaaac tggctaaatg
      541 tctattttgg caacctggtc ggcgcactgc tgtttgtact tttaatgtgg ctttccggcg
      601 agtatatgac cgcaaatggt caatggggac taaacgtcct acaaaccgcc gaccacaaag
      661 tgcaccatac ttttattgag gccgtctgcc ttggtatcct ggcaaacctg atggtatgtc
      721 tggcagtatg gatgagttat tctggccgta gcctgatgga caaagcgttc attatggtgc
      781 tgccggtcgc gatgtttgtt gccagcggtt ttgagcacag tatcgcaaac atgtttatga
      841 tcccgatggg tattgtaatc cgcgacttcg catctccgga attctggacc gctgtcggtt
      901 ctgcaccgga aaatttttct cacctgaccg tgatgaactt catcactgat aacctgattc
      961 cggttacgat cggtaatatt atcggcggtg gtttgttggt tgggttgaca tactgggtca
     1021 tttacctgcg tgaaaacgat caccattaat ggttgtcgaa gtacgcagta aataaaaaat
     1081 ccacttaaga aggtaggtgt tacatgtccg agcttaatga aaagttagcc acagcctggg
     1141 aaggttttac caaaggtgac tggcagaatg aagtaaacgt ccgtgacttc attcagaaaa
     1201 actacactcc gtacgagggt gacgagtcct tcctggctgg cgctactgaa gcgaccacca
     1261 ccctgtggga caaagtaatg gaaggcgtta aactggaaaa ccgcactcac gcgccagttg
     1321 actttgacac cgctgttgct tccaccatca cctctcacga cgctggctac atcaacaagc
     1381 agcttgagaa aatcgttggt ctgcagactg aagctccgct gaaacgtgct cttatcccgt
     1441 tcggtggtat caaaatgatc gaaggttcct gcaaagcgta caaccgcgaa ctggatccga
     1501 tgatcaaaaa aatcttcact gaataccgta aaactcacaa ccagggcgtg ttcgacgttt
     1561 acactccgga catcctgcgt tgccgtaaat ctggtgttct gaccggtctg ccagatgcat
     1621 atggccgtgg ccgtatcatc ggtgactacc gtcgcgttgc gctgtacggt atcgactacc
     1681 tgatgaaaga caaactggca cagttcactt ctctgcaggc tgatctggaa aacggcgtaa
     1741 acctggaaca gactatccgt ctgcgcgaag aaatcgctga acagcaccgc gctctgggtc
     1801 agatgaaaga aatggctgcg aaatacggct acgacatctc tggtccggct accaacgctc
     1861 aggaagctat ccagtggact tacttcggct acctggctgc tgttaagtct cagaacggtg
     1921 ctgcaatgtc cttcggtcgt acctccacct tcctggatgt gtacatcgaa cgtgacctga
     1981 aagctggcaa gatcaccgaa caagaagcgc aggaaatggt tgaccacctg gtcatgaaac
     2041 tgcgtatggt tcgcttcctg cgtactccgg aatacgatga actgttctct ggcgacccga
     2101 tctgggcaac cgaatctatc ggtggtatgg gcctcgacgg tcgtaccctg gttaccaaaa
     2161 acagcttccg tttcctgaac accctgtaca ccatgggtcc gtctccggaa ccgaacatga
     2221 ccattctgtg gtctgaaaaa ctgccgctga acttcaagaa attcgccgct aaagtgtcca
     2281 tcgacacctc ttctctgcag tatgagaacg atgacctgat gcgtccggac ttcaacaacg
     2341 atgactacgc tattgcttgc tgcgtaagcc cgatgatcgt tggtaaacaa atgcagttct
     2401 tcggtgcgcg tgcaaacctg gcgaaaacca tgctgtacgc aatcaacggc ggcgttgacg
     2461 aaaaactgaa aatgcaggtt ggtccgaagt ctgaaccgat caaaggcgat gtcctgaact
     2521 atgatgaagt gatggagcgc atggatcact tcatggactg gctggctaaa cagtacatca
     2581 ctgcactgaa catcatccac tacatgcacg acaagtacag ctacgaagcc tctctgatgg
     2641 cgctgcacga ccgtgacgtt atccgcacca tggcgtgtgg tatcgctggt ctgtccgttg
     2701 ctgctgactc cctgtctgca atcaaatatg cgaaagttaa accgattcgt gacgaagacg
     2761 gtctggctat cgacttcgaa atcgaaggcg aatacccgca gtttggtaac aatgatccgc
     2821 gtgtagatga cctggctgtt gacctggtag aacgtttcat gaagaaaatt cagaaactgc
     2881 acacctaccg tgacgctatc ccgactcagt ctgttctgac catcacttct aacgttgtgt
     2941 atggtaagaa aactggtaac accccagacg gtcgtcgtgc tggcgcgccg ttcggaccgg
     3001 gtgctaaccc gatgcacggt cgtgaccaga aaggtgctgt agcgtctctg acttccgttg
     3061 ctaaactgcc gtttgcttac gctaaagatg gtatctccta caccttctct atcgttccga
     3121 acgcactggg taaagacgac gaagttcgta agaccaacct ggctggtctg atggatggtt
     3181 acttccacca cgaagcatcc atcgaaggtg gtcagcacct gaacgttaac gtgatgaacc
     3241 gtgaaatgct gctcgacgcg atggaaaacc cggaaaaata tccgcagctg accatccgtg
     3301 tatctggcta cgcagtacgt ttcaactcgc tgactaaaga acagcagcag gacgttatta
     3361 ctcgtacctt cactcaatct atgtaattag atttgactga aatcgtacag taaaaagcat
     3421 acaataaagg ctccacgaaa gtggggcctt ttttagcacg agagcctttt ttgtcagcta
     3481 tctatacttt aaggtgactg ccaaaacaga ctcgacgtag ccttcgagct gcgcaccaac
     3541 acggcctcag atgggccaca tctggagaaa caccgcaatg tcagttattg gtcgcattca
     3601 ctcctttgaa tcctgtggaa ccgtagacgg cccaggtatt cgctttatca cctttttcca
     3661 gggctgcctg atgcgctgcc tgtattgtca taaccgcgac acctgggata cgcatggcgg
     3721 taaagaagtt accgttgaag atttaatgaa agaggtggtg acctatcgcc actttatgaa
     3781 cgcttccggc ggcggcgtta ccgcatccgg cggtgaggca atcctacaag ctgagtttgt
     3841 tcgtgactgg ttccgcgcct gcaaaaaaga aggcattcat acctgtctgg acaccaacgg
     3901 ttttgttcgt cgttacgatc cggtgattga tgaactgctg gaagtaaccg acctggtaat
     3961 gctcgatctc aaacagatga acgacgagat ccaccaaaat ctggttggag tttccaacca
     4021 ccgcacgctg gagttcgcca aatatctggc gaacaaaaat gtgaaggtgt ggatccgcta
     4081 cgttgttgtc ccaggctggt ctgacgatga cgattcagcg catcgcctcg gtgaatttac
     4141 ccgtgatatg ggcaacgttg agaaaatcga actcctcccc taccacgagc tgggcaaaca
     4201 caaatgggtg gcaatgggtg aagagtacaa actcgacggt gttaaaccac ctaagaaaga
     4261 gaccatggaa cgcgtgaaag gcattcttga gcagtacggt cataaggtaa tgttctaatt
     4321 tccccgtaaa gcggcaactt tattgagttg ccgctttttt attccgcgct tcattctgct
     4381 acagcgtttt taacacattt tatagtgcat ctaccatatc ccgtacctga gataataacg
     4441 actgataata gctcgcatga agttcttgcc catcgaatac cactgtatta cacaagaaag
     4501 tgacatcggc atcttcaata ccaagataac tgcacatatt tttcaaataa tcgcttatat
     4561 ttttttccca gcccatctgg acaaatgatt ctttgtctcc tccaaccagc gccacccaac
     4621 gaactttatt gaatggtaat ttgtgcccat ctccataagc cagcccatta ttccatactc
     4681 tgtcaatata tccttttagc attgccggga agctatacca ccagagagga aaaaccacca
     4741 ctaacgtgtc atgttcaagc agctctgaat aaagctgatg aacctctgga gaataacgtt
     4801 tatccatatt cttccagtct ggttcatctt ccggcgtcat cactgggttg aaattacgcc
     4861 gatataaatc gagttccgtc acctgtattt tccgctccat tgcccgctga tggatcgcat
     4921 cagcaatatt tgcggtcaat gaatcatgac gaggatgggc ccataccaaa taaatacgtt
     4981 cagactgcat aattttacct ccacgttaag ttctggagcg cactttacgc aggcggcata
     5041 atcctgtaaa cgtgatgatt caggatgagt taatgaagga ttttcactaa tgcggatgaa
     5101 tatgtctgac tttgccactt tctttgccgt cgcccgtaat caaagctttc gtgcagcggg
     5161 cgatgagtta ggcttatcct cgtccgccat tagccatagt attaaaacac tggaacaacg
     5221 tcttaaaatt cgcttattca atcggacaac ccgaagcgta tcgttaacgg aagcgggttc
     5281 taatctctat gaacgattgc gcccagcttt cgacgaaatt cagatcatgt tagatgagat
     5341 gaatgatttt cgtctgacac cgacaggaac gctcaaaatc aatgccgccc gggtagctgc
     5401 acgtatattt ttaatgcctt tactggttgg tttcacacgt gaatatcctg atattaaagt
     5461 cgagctcaca actgacgatt ctcttgtcga tatcgtccaa cagggctttg acgctggcgt
     5521 tcgacttagt ggcatcgtcg aaaaagatat gatcagcgtg gcgatcggcc caccagtgaa
     5581 attatgtgta gcagcaacgc cagagtactt tgcaagatat ggcaaacccc gccatcctca
     5641 tgatctgctt aatcatcaat gtgttgtttt tcgttatccc agcggcaaac ctttccactg
     5701 gcaatttgca aaggagctgg aaattgctgt cgcaggaaat atcattctgg atgatgttga
     5761 tgctgaactg gaggccgttt tgatgggtgc tggtatcggg tatctgctct atgaacagat
     5821 caaggagtac cttgatactg gtcgtttaga atgtgtactg ggagactgga gcacagagcg
     5881 tccaggcttc cagatctatt atccaaaccg ccagtatatg tcctgcggct tgcgcgcatt
     5941 tctcgattat gtaaaaacgg gtcagatctg ccagagtcag cgtcaccgac cacaataatt
     6001 atttactatt cgctttacgc gccagcagcg ggaaaatcag tcctaatcct accagcacaa
     6061 acggcgttgc aacattcagc gccaactgga aggtccactc tgcagtgaag gcttccattt
     6121 tcgggaagat ccccgtcaaa caggcaaagg cggtaaaggc aaaacaccaa atcccgacgg
     6181 tcattgccag aggcttatta cgaataaaga catattctgg tttatatttc tgcgccaggc
     6241 gaacgactgc aataaatgca acaaataccc ataaataacg cagcggcatc actaccgagt
     6301 taagattcaa cagccattta tagagattgt tcatatcacc gatgccgaga gtcggcagca
     6361 taatcagaat tgccaccagc accagggtca gaaaatagcc attaacgggc gtaccagaag
     6421 cgttggtacg acataaactg gctggaatat atttgctgtc agcatcacct aatagcactt
     6481 taagcggggc atcaatcgag aataccagcg cagctacttg tcccagggta ttcgcaatgg
     6541 cgtaaatcac cattaaagta ttacccatgt tgtaatactc gcccagcttc tgaaaggcgt
     6601 aatattgacc gttagtcatt aagtcatccg ggatattacg cgagtcaaac atcatcccca
     6661 tcgccagtga gcccagaatg gcacaaaccg caaccatcac cgccaggcat agcatccctt
     6721 ttggaaattc ttttcctggg ttgcgcgttt gattaacgta aggagaaatc ttctctgctc
     6781 cgccaaccgc gaaaaccagc attgaaatag tggtaatgta ggtaaagtcg atatgaggaa
     6841 tgaacgtttc ccaggtaatg tttgtggtcg caatatgcac ttcagtaatc gcagacgcgg
     6901 ttaccgccat cgccacatac aggagcgaca taacaaacat cgcaatccct gccacagaac
     6961 cgacgatttt cagcgatttc ataccgcgtg aagcaaccca cataaagaag ataaacagca
     7021 ccagcgttaa cccctgtaac gctacgactg aatattcttt gattagcgaa ccgtcgcctt
     7081 tcatcgccca accgagcgca atcagaattg cctggggttt ttgtgccaga aagggaatat
     7141 gcaccaccca gtaggtccac gcggcgagat aagccagtcc gggtcccatc gtgtgtttaa
     7201 tccaggtact gaccccgccc ttcccatctt tgaaggtcga gcctaactga ccaacaatta
     7261 gcgcataagg tgtgaaatag agtgcaaaga taaacaccca tgaaaaaaca accaccagcc
     7321 cctggttggc atagttatta acaacgttgc caaaacccca gacagtgata aaagacatca
     7381 gcgcaatgtt gtaccatcgc aactgttttt cctgaacatt cccagccata gacaaatcct
     7441 tcgccaaaat taaaggggca tctgtaaaac gagggattat gcgcggcaaa taagaagtgc
     7501 ggaaatagcg aaatgcaaaa ttataacctg ctgcacattt tatttttaaa cgaataaaat
     7561 ttggaataaa gcaagtcaga tagaagaaaa aaaaagcaga gtgtgaaaca gtaagttcac
     7621 actctgcttg aattcattta cacgtgagca acgggtttcg gcgtatgacc ggcgttgcgc
     7681 agcagcatca gcaaatagat aaacgatacg ctggcgatca tgataaacaa taaattatcg
     7741 gagaaattct gcattagcat agcggtaaat gacgggccaa gcagacttcc cacagtatag
     7801 ctcaacagta aggcctggtt catcgccacc agttgatgat gttcaacttt ctcgcaagcc
     7861 catgccatcg ccaccggata tagcgtaaag ccagcggcac cgaggatgaa taacgctggg
     7921 gccatcgccg cctggctaag catcgcgata ctgccgagaa tgacgacaaa gacctgaaca
     7981 cgcaacacca gcagtcgacc aaacttatcc gccagacgtc caatcggcca ttgtccaagg
     8041 atacccgcac tgaccagtac cgccatccag aaaccaatgc tggcattgct cacccctttg
     8101 tgattgaggt acagcggcat caggccatat agagatccca gaacgattcc tgagataatg
     8161 cagccattca cgccaagccg cgcctgacgg agttttagca ttgacgtaat tgacgtcgaa
     8221 tcatggtttt cagcctgctg attcagcaca cgcgtaaaca acagcggtaa gatccctgcc
     8281 aacgtcaaac ctgtaaccca cggcaatacg gacatcagct cggttgaaac tttgctgacc
     8341 agtaactggc ctaaaaacgt tcccacgtaa taaaccatca tatacgcagc aagcaaacgc
     8401 ccacggttac gtgacgtccc actgcacatc agcgcgctct caacaaccac ccaaatcatg
     8461 gcacagccga cgcccgcgac aaaacgccaa gccaaccagc tccagaatcc aatcatcagg
     8521 ccaaggccgg cacagccagc ggcaaaaatg aaggaggcca gataatagct gcggttaaag
     8581 ccaatgcgct taatgacata ccctgtcagc aatgtaccga caaggttgcc ggtaaaatag
     8641 gatgagctga caacgcctac ctgccatgtg gacatgtgtt cctgggcgag ccaaagcggc
     8701 acgagtgtat ttaacaccgc gatcgccaga gtcaacaaaa gcaggccaga cagcaaaagc
     8761 atgacaggcc gggtatacgt ggacatgaat taaaaccgtg aggaagttca aatttcatgc
     8821 gcatcatgcc accgccaaaa acattgtcaa tgggtgaaaa tacgcgcata tcgcagcggc
     8881 ggtattatcg atttatatta ctcattcata acgctaatgc tgaagagatg attaatctca
     8941 tctgattgtt tttattgatc taacacaaat ttcatacgct tcgttcagtc gaaaaatttc
     9001 atggtcacag caaactcata tttcccgtct atgctttcag agcaatgcat cacgtctgac
     9061 cgatttcaga cgtaccactg actctcgaaa ggagaagagg atgaccaaac cgtatgttcg
     9121 tcttgataaa aatgatgctg ccgttttgct tgttgatcat caggctggtt tactttccct
     9181 tgtacgggat atcgaacccg ataagtttaa aaataacgtg ctggcgctgg gtgacctggc
     9241 taagtatttc aacttaccta ctattcttac caccagtttt gaaaccggtc ctaacggtcc
     9301 attagttcca gaactgaaag cacaattccc cgatgcacct tacattgctc gcccgggaaa
     9361 tattaacgcc tgggataacg aagattttgt aaaagcagtc aaagcgacag gtaaaaaaca
     9421 gttaattatt gccggtgtgg taaccgaagt ttgcgtggca ttcccggcgc tttcagccat
     9481 tgaagaaggg tttgatgtct ttgttgtgac tgacgcgtca gggaccttta atgaaattac
     9541 ccggcattcg gcatgggatc gcatgtcgca agcaggtgcg caattgatga catggttcgg
     9601 cgtggcgtgc gagctgcacc gtgactggcg taatgatatt gaaggactgg cgacgttgtt
     9661 ctctaaccat attcctgatt atcgtaattt gatgaccagt tatgacacgt taacgaagca
     9721 gaaataaaag agaaccgggg ccgaaacccc ggttgttact gattagcttg cgacggccat
     9781 ccccacggtc atatgcaaac catagaatac gccgcgaccg attaattccc ctgccagaag
     9841 cagaatgaat gagacggata gtagcggaac cgcaggctga taacctttta gctgtggtgc
     9901 aatccacaag cacagggcaa cggccaaaag cacgatccgc caggacatca gcgcaccata
     9961 gtccggcacc agcgctgcgg cctgctgcac agaactatga atagttgcca gctctgtgcc
    10021 ctgcatcact gacatcacgc cactcaccac cagtgccagt acagaaatgg ctggcagcag
    10081 acgcatcgcc cagccatcga cgccagccag actcaacaac aggtaaccca gtaacgggcc
    10141 gcccataaac atcgtcagga agaagcccat cggcgtccag atgctgtacc aggtcggaac
    10201 ggtatcaatg ctgttataca cacgcaccat catccagaca aagatgacgc caagaaccat
    10261 cgtcactatc agccacagcg tacgcaatgc cggtgacagc tttttcagca ttgccagcaa
    10321 ccagccgatg ccgcctacgg caaaaaagat cgaaccgctg gcgatttcgt tactgagtgc
    10381 tgaagcccct acccggttga gcgagttaaa agcgcgcatt ggtgaaccaa gatggagcat
    10441 agaggcgata aagccaatgc ccatcagcac ccataaacca aacatgcagg cgataacacg
    10501 ctgctgggct tctgctcgca ggtcgccttt gagcagcgcc aaagccagaa cgataaaacc
    10561 acctgctaca cattgcccga agaccgtgaa gatcatcagc ggccattcat gccatccact
    10621 tcccatctca cacctccttc gggtttgcca gatagccagt ggtatccccg gtcgggcggc
    10681 tattggcatt gggtttgatc acaatattcg gtttggtaaa gtgagctcgc ggcaacggcg
    10741 caacggccgc cagatcgcca tgttttttac gcagctcgtc gatagggccg aaatccagcg
    10801 cccgcagcgg acaagattca acacagatcg gctttttacc ctccgcaaca cggtcataac
    10861 aaccatcgca tttggtcata tggcctttcg tttcgttata ttgcggtgcg ccatacgggc
    10921 aagccatatg gcagtagcgg cagccaatgc acacatcttc atcgaccaca acaaaaccat
    10981 cttcacgttt atgcatcgca ccgctcgggc agactttagt acaagccgga tcttcgcagt
    11041 ggttacatga aatcgacaga tagtaggcaa acacgttctg gtgccagaca ccgttatctt
    11101 cctgccagtc gccgccagca tattcataaa tgcggcggaa gctgacttct ggcgtcaaat
    11161 ctttgtagtc tttacaggcc agctcgcagg ttttgcaacc ggtgcaacgg ctggaatcaa
    11221 taaaaaatcc atactgggtt gtcatcggtt actccttaca ccttttcaac ctgaacaagg
    11281 tttgtatgtg acggattccc cttagcgaga ggagacggac gttgagtggt cagtacgttg
    11341 atacaaccgc ccttatcgac acgttttgta tccgggtcat accaggcacc ttcacccagt
    11401 gcgaccacac ccggcatcat tcgtggcgtc actttcgcct caatatgaac ctcaccacga
    11461 tcgttaaaga tcctgacttt atcgccgttg ttgataccgc gtttttgcgc atcaagcggg
    11521 ttgatccaca tttcctgacg gcaagctgct ttcagcacat caacgttgcc gtaagttgag
    11581 tgaacgcgag atttatagtg gaaacccgta agctgcagcg gatactgttt gttcagcgga
    11641 tcctgataac tttcaaagcc cggcgtgtag atcggcagtg gatcgatcac atcgccttct
    11701 ggcaattccc aggtagcggc aatgtcagcc agcgcctgcg aataaatctc aattttgccc
    11761 gatggcgtag tcagtgggtt tgcctgcgga tcttcacgga aggctttata agcaacgtga
    11821 tgcccttgtg ggtcacgctt tttaaagatc ccctgcttgc ggaactcttc aaacgttggc
    11881 agttcaggaa tcgcttcccg cgactgggca tacagatgcc gcatccactc ttcctgggta
    11941 cggccttcag taaactgttg ctcaacgcca agacgttttg ccagttcgct ggtcatttca
    12001 tagatggtct tacattcaaa gcgcggttta atcacctgat cgttgaaaat cacgtaagac
    12061 atattcccgc aggatgcatc cagtgcaaag tccatctgtt cggaagcggt gcagtcaggc
    12121 agcaggatgt cagcatattt cgccgatgag gtcatgtggc agtcgataac cacaatcagc
    12181 tcgcacttct tatcatcctg aaggatttca tgggtacggt tgatttcaga atgctggtta
    12241 atcaggcagt taccggcata gttccagatc attttgatcg gcacatccag cttatctttc
    12301 ccgcgtacac catcacgcag cgccgtcatt tccgggccac gttcaatggc atcggtccac
    12361 ataaacatcg aaatgctggt ctggatcggg ttttccaagg tcggcatacg gacaaacggt
    12421 aagctgtatg aaccttcacg cgcgccgctg ttgcctccgt taataccaac gttaccggtc
    12481 agaatcgcca gcatcgagat agcacgggtt gcgatttcac cgttagcgtg acgctgcggg
    12541 ccccatccct ggctgataaa cgccggtttg gtactgccga tttcacgagc cagtttgatg
    12601 attttgtctg ccggaacacc ggtgatttgt gaggcccatt ccggtgtttt agccacgcca
    12661 tctggccctt cacccagaat ataagcttta tagtggccat ttttcggcgc actggctggc
    12721 agggttttct catcgtagcc aacgcaatat ttatcgagga atgcctgatc caccaggttt
    12781 tcagtgatca tgacgtacgc cagaccgtta accagtgcgg catctgttcc cggacgaata
    12841 gggatccact catcttcgcg cccggcaccg gtgtcggtat agcgcggatc aatgatgatc
    12901 atgcgggcat tagatttctg gcgtgcctgt tcaagatagt aagtcacccc accgccactc
    12961 attcgcgttt cgccagggtt attaccaaac agcactacca gcttactgtt ttcgatatcc
    13021 gacgggctgt tgccatctgc ccagccgccg taggtatagt tcaaaccttc cgcaatttgc
    13081 gcggaggagt agtcgccgta atggttcaga tagccgccgc agcagttcat cagccgcgcg
    13141 accagggtat ttcccggcgg ccaggagcgg gtcatggtgc cgcccagcgt accggtgcca
    13201 tagttcagat agatagactc gttgccgtac tctttgatca ggcgctgcat attggtcgcg
    13261 atgatgtcgt aggcttcttc ccagctaatg cgctcgaatt tgccttcacc gcgcgccccg
    13321 actcgtttca tcggatattt caggcggtcc ggattgtaga cacggcgacg catggaacgc
    13381 ccacgcaggc aggcgcgaac ctggtgcagg ccgtcgtaat tgtcatcgcc ggtattgtcc
    13441 gtttcgacat atttgatttc accgtccacg acgtgcatac gtagcgggca gcgactacca
    13501 cagttaactg tacaggcgct ccagataacc ttttcgtctg attttgttgg aatggcgcta
    13561 tcgacagcgt gcgcaatccg actaaaaggt aatgttaatg cgctgctggc cattgccagg
    13621 ccgccgatcg ctgtcgtttt taccaaacca cggcgactca cctcagcagc caataccgca
    13681 tcagggattt tcgttttcat aatggctcac tcaagcttgc tcacgattga ttccagtaac
    13741 aaaccgtatt gtgtaattat ttctatagca gctcgctata taggcttgta tacatcgaat
    13801 aatttgaaaa tcaaagagat aggtagattt tcactcgaaa gtagtattac gtgtaagtga
    13861 ggagtaatta ttgttcggta tcaaagggag acaaaaaaag cgcccgcagg cgctttttag
    13921 attcagaaaa attgggtatt agccaatata ttccagtccg ttcatatacg gacgcagaac
    13981 ttctggtact tcaatacgac catcagcctg ctgatagttt tccattactg caaccagcgt
    14041 acgaccaaca gccagtccag aaccgttcag ggtatgaacc agacgggttt tcttgtccga
    14101 cttgctgcgg cagcgtgcct gcatacgacg cgcctggaaa tcccaaacgt tggagcagga
    14161 agagatctca cggtaggtgt tctgtgccgg gatccatact tccaggtcgt aagttttgca
    14221 agcgccaaag cccatgtcgc cagtgcaaag gatgatttta cggtacggca ggcccaacaa
    14281 ctgcaggact ttttctgcat gaccagtcat ctcttccagc gccgccattg agtcttccgg
    14341 gcgcacgatc tgcaccattt caactttgtc gaactggtgc atacggatca gaccacgggt
    14401 gtcacgacca tatgaaccgg cttcagaacg gaagcatggg gtgtgggcgg tcatcttaat
    14461 tggcagatca tcttcatcga tgatttcacc gcgcaccagg ttagtcagcg gaacttctgc
    14521 cgttgggatc agcgcatagt tactggtgtc tgcttcttct tccagcggac gagtatgaaa
    14581 cagatcgcca gcaaatttcg gcagctgacc cgtaccgtac agcgtgtcct ggttaaccag
    14641 gtacggaaca tagttctcac tgtagccatg ctgttcggta tgcagatcca gcataaactg
    14701 cgacagtgcg cggtgcatgc gagcaatctg ccctttcatt accacaaagc gggaaccagt
    14761 cagcttaact gcagctgcaa agtcgaggcc agagtgcatt tcacccagcg tcacatggtc
    14821 acgaacttca aagtcaaact cacgcggggt gccccagcgg ctgacttcga cgttgtcatt
    14881 ttcgtcttta cctaccggca cttcatctgc aggcaggtta gggatggtca gcgcgatatc
    14941 gcgaatttca gcctgtaaag catccagctc ggcttttgct gcatccagct cttcgcccag
    15001 tttgttcact tccagacgta aaggctcgat atcttccccg cgcgctttcg cctggccaat
    15061 ggatttcgat cgggagttac gctccgcttg caggttttcc gttttgacct gcaatacttt
    15121 acgacgctct tcaagagcgc ccagcttatc tacatccagc ttaaagcccc ggcgtgccag
    15181 tttttcagcg actgcgtctg gctcattacg cagcagattg ggatcgagca tgcttatcct
    15241 gtgcttatcg aattaaatgg gagatgtggc cacagcctgc gaccacaggg atacagtaac
    15301 aacattaccg caacgataac attaacggta gcgttttatg gggctatttt gatcctgttc
    15361 agccagccag gcgagctttt cgccaatctt gccttcaagg cccctgtttg tcgggaaata
    15421 atagcgtgtt tgtgctattt ccggcgggaa gtaaacctca ccggcagcat aagcgtttgc
    15481 ttcatcatga gcgtaacgat attcctgccc gtagcccatt tccttcatta atttcgtcgg
    15541 cgcattacgc aaatgaaccg gcacgtcata atccggacgt tcgcgagcat ccgccagcgc
    15601 agctttaaac gcagtgtaga cagcgttgct ttttggcgcg caggccaggt aaacaatcgc
    15661 ctgagcaatg gcgcgttcac cttccgccgg gccaacgcga gtaaagcaat cccaggccgc
    15721 aattgccacc tgcatcgccc gtgggtcggc attgccgaca tcttcggaag caattgccag
    15781 gcaacgacgc gcgacatata acggatcgcc accagcggta ataattcgcg cataccagta
    15841 gagcgccgca tcgggtgcgc taccacgtac cgacttatgc agtgcggaaa tgagatcgta
    15901 aaagcgatcg cctttgttat caaagcgggc gcttcgctca ccggcgattt cggtcaataa
    15961 ttcaggcttc aggacccgct taccgctatc attgacttcg gccatatccg ccatcatttc
    16021 cagcgtattt aacgcccggc gcgcgtcgcc attcaccagt tcagcaatgg cgcgtcgtgt
    16081 ttcatctggc agaacaatat cctgaccacc ataaccacgg gtcttgtctt ccatcgcctg
    16141 agttagtact tgctcaatat cctctgtacc cagggatttc aacagataga cacgggcacg
    16201 ggaaagcagt gccgaattaa gctcaaacga cgggttttca gtggttgcgc caataaaagt
    16261 gatggtgccg tcttcaatat gtggcagaaa tgcatcctgc tggcttttgt tgaatcggtg
    16321 cacttcgtca acaaaaagaa tagtgcggcg acctgcattg cggttttgcc gggcgcgctc
    16381 gatcgcctcg cgaatctctt tcacgccaga ggtgacggca gaaatacgtt ccacatcagc
    16441 gttcgcatag cgggcaatca cttcagcgag ggttgttttg ccggtacccg gtggccccca
    16501 gaggatcata gaatgtaaat gcccggcttc gatagcgcgc ggcaacggct tccccgcagc
    16561 cagcaaatgt tgctggccga tatactgtgc taaattttct ggccgcatac gcgcggccag
    16621 aggttgaaaa gtattatccg aaaaatcgag cgacagattg ctcactcagg tgcctctact
    16681 tacgttgatc atctaccgtg acgccttgcg gcggggtgaa ggtaaatttc gctgcatcca
    16741 cagccccatt ttgctgggat ttcagttgat aactgctgcg ctgatcgtcc tgctccaccg
    16801 cgctaaactg atggattgtg ccatcacgtc ccacgttaat ggtgaactgc ttcagattgc
    16861 cattgctggc tttcggcgtc aggacaaagt catcgccatt ctgtttgata ttgtactgct
    16921 gccagtcgct ggactggttg cgggcaatca gcataaacgg cgtattaccg gtggcatctt
    16981 tcagccaggt tgccgtagct tgctcaacga acgggttata gaaccacagt gttttaccgt
    17041 cagaaaccag aatgctttca tcaggttgtg tcatatgcca gttgaataag tttggacgtt
    17101 tcacccacag atcgccctga ccttcctgca ccgccgcgcc gctaccgtca gtcacttttt
    17161 gtgtgaagct ggcgtggaag ctgctgactt tatccaggcg gcttttcaga tcgcttgcgg
    17221 catcagccca aacgctgctt gctactaagc ttgagagtaa tgcacaggtg atggcaattt
    17281 ttttcatcat tattcctcaa attacgtcac tcccgatcgg gagttccctc tactctctat
    17341 tccaggctaa taatcagctt gaggaaagaa gaaaatactg aattttcagt tgatagagtg
    17401 ccggatgcga cgctaccgcg ccttatccgg caatgcatta attagtcaaa cggcggtggg
    17461 gccagcactt cacgattacc gttgtgcccc tgttcgctga caatcccctg cgcttccatc
    17521 tgttcgataa tacgcgctgc acggttataa ccaatgcgga actgacgctg tacgccagaa
    17581 attgacgctt tgcgtttttc agtgacaaac tgcaccgcct gatcgaacaa cggatccagt
    17641 tcttcagcgc catcgaaacc gcccgcgcca ccttcgcttt cactgtcgga agtgatgcca
    17701 tcaacatact gtgggcgacc acgcgctttc cagtcctgca ccacggcatg aacttcctga
    17761 tcgcgaacaa aagcaccatg gacacgtacc ggcaacgtgg agttcggccc agagtagagc
    17821 atatccccca tccccagcag tgattccgcg ccagcctgat caagaatggt acgtgagtca
    17881 atcttactgg atacggtaaa ggcgatacgg gtcggaatat tcgctttaat cagaccagta
    17941 ataacatcaa ccgatggacg ctgagttgcc agtacgaggt ggatacccgc ggcacgggct
    18001 ttttgcgcca gacgtgctat cagctcttcc acttttttac ctaccgtcat catcaggtcg
    18061 gcaaattcgt caaccaacac cacaatgtat ggttcttttt tcagcaccgg atgctgggca
    18121 tccatactgt cacccggctt ccagtacggg tctggaatcg gacgcatcat gcgatcggct
    18181 tcagcaattt tttcgttata acccgccaga ttacgcacac ccagcgcaga catcagttta
    18241 taccgacgct ccatctcgtt aacacaccag cgcagcgcgt tggcggcatc tttcatatca
    18301 gtaacgactt ccgttaacag atgcggaatg ccttcataaa ccgaaagctc cagcattttc
    18361 gggtcgatca tgatgaaacg cacatcttct ggctgtgctt tataaagcat gctcaggatc
    18421 atcgcgttca caccgacaga tttaccggaa ccggtagtcc ccgcaaccaa caagtgcggc
    18481 attttcgcca gatcggcaac caccggctca ccggcgatat ctttacccag caccacggtt
    18541 aatggcgacg gattatcgcg gaatttggcg ttatccaaaa cttcgcgcag ataaacggtt
    18601 tgtcgttttt tatttggtaa ctccagacct acatagggtt tgccaggaat aacttcaacg
    18661 acacgcaccg ccaccgtcga aagtgaacgg gcaaggtccc gtgacaagtt agaaatgcgc
    18721 gccgctttta cgccaggagc caggttaagt tcaaagcgag tgataaccgg ccccggagag
    18781 taattgacga catcggcttt aatacggaaa tcagccagac gcgcttccac caggcgagcc
    18841 atttgttcaa gcgcaaaggt atctaccggc tccacttcgc tcggcggcgg tgtcagcaaa
    18901 tccagagaag gcagcggcgt cgtcggttta tgcaacggac ggctgtcgcc attacgcatc
    18961 aacagcggat gaagcagggt atcctgctgc tgcggtgcaa ccggttgttg cggctgttga
    19021 tactgcggct gcggagcaac tggttgttgc ggctgctgat attgcggctg tggcgcaacc
    19081 ggttgttgcg gctgctgata ttgtggctgc ggcgcaacct gctgttgtgg ctgctgatac
    19141 tgcggctgcg gcgcaaccgg ctgttgcggc tgctgatatt gctgctgtgg cggaactggt
    19201 tgttgcggct gctgatactg ctgctgcggt gcaaccggtt gttgcggctg ctgtacaggt
    19261 tcaacaattg gcgtaaacaa cggttcgtgt ggaccatcat ccagcaacgc tttcattggc
    19321 gaaaattcaa aatcatccag cgagaacgga ttcgccccag ccggttgttc gccggaataa
    19381 cgttgttgtt gagtttgcgc aaactgacga gccagttcag cctctgccgc agcatctgca
    19441 tcttctgcgt ttacgggcac atcatgttga tactgttcgc catagcgttg ctgctgtgtc
    19501 tgggcgaact gacgtgccag ttcatcctgc tgcatcgcat cgatttcatc atcgttatac
    19561 tgatcgccag aatcatactg attgcgctgg gcttcacggg ctttctcttc cgccgcacgc
    19621 tgtgagggca gcttaatacc gtaagacgcc agttcacgac gagttggcac gcggatacgt
    19681 ttcggtcgtg gcaactgcgg gccaatcccc tctttgactt gcggacgcgg tccaccgcta
    19741 tttgccagac tgaagactgg cgcggcaacg gttgcggcag cccccgtcgc cagtgtcgct
    19801 tttttcacgc cagatgccag cggagaaaca gcggcagcgg cttctactgg aggtactgct
    19861 gcaacagaag gcgctttcaa cgaagatttg atcggttctg gttctttaac cggttctgga
    19921 atcggttgat accaggccgc aagttgttca cgttcacggg ctcgcttctc ttccacttct
    19981 tcaaaatagt aaagcggcgg acgcgcgggt tttgtctctt ctacaacggg ttcaggctcc
    20041 acaacaggct gctgttcaac ggattgcggc tgttggtaca acggctcctg agcggctggt
    20101 tgctgataag tttgctcagt ctggtatgta gactgtggag caaaagtgga ttgctgctct
    20161 tcggcttgcc aggcgttacc tgccaccggt tgttctggcg caggggcgta atacggctgt
    20221 tgtgcaggct gttccggcgc agttgcgtaa tacggttgct gaacaggttg ttccgccgca
    20281 ggagcgtagt aaggttgttg cgcaggttgt tcagctgcag gtgcataata cgactgctgc
    20341 ggctgtactg gttgttgcag cggctcatta tattgcactg caggctgcgc atattgtgac
    20401 tgctgtgggt aaccttccgg tgcaggagca ataaccggct ctcccgtttg tggacccggt
    20461 acaggctgcc aggctactgt aggttgcgag ggtggaacat caacagaggc aacaggcggc
    20521 gtctgagtca caggttcaac cggcgcagcc cagctttgtg tcgccgtggt agcagcagct
    20581 gctacagcga caggttcggt aattggcgca ccgtttaata atggatcgta ttcgtcatat
    20641 tctggctgcg ttgcacgatt gcccgaaaat aggacgtcgt ccgggtcggc agccacaccg
    20701 cgtgcagtgt aggtaatctc ttcgtcatca tccatccgct taccggagaa caacgcagcg
    20761 tctgtttgcc gccccatcgg attaatgaat ttttccgcca accgtttacg acgcgctagc
    20821 gcgccgcgaa gaatacgggc acggcgtgat tcatgctgtt tgccgtggtt ttcatcttca
    20881 tactcttcgt catcttcata ttcatcttca tcgacccagg tatcatcgcg acgggtacga
    20941 ttactggcga aggtgagaat gtttaaaatc cagccgccga gtttttcagc aatggtcacc
    21001 catgaccaac cggtgaacag cgtcaggccc gcagcccaaa cgcagagcag cgcaatagtt
    21061 cccccgctac tgtgtagcag tggttgtagc gtagtactta gtaagctgcc aatgacgcca
    21121 ccggaggcaa aataccagat atcgtcagcg ttgattgccg ccagaccaca ggaggtaagg
    21181 atgagcgcca aaacgccaat gatgcgtagc gaaacggcaa aataatcaat gtattcgtcg
    21241 ctggactgat gacgccaggc aaaccaacaa ccgccgacaa taatgacggg aatggtgtaa
    21301 gccatcacgc caaaaataaa gaacagcgta tctgccaacc acgcaccggg catcccacct
    21361 aaattatgga taggttcatg ccaggccgtt tgcgaccagc tggggtccga agggttaaag
    21421 cttagtaagg cagccatcaa ccagacggca aacaggacaa taaggatcag caacgcctcc
    21481 agaaggcggc ggccgctact taactttgtc aatgtgactt ctttgtcttc agtgtattcc
    21541 tggctcaaga aaggctctcc aggttacagg cttttcctgc ccgatgctaa aacggacaac
    21601 agtaccaggt taccccagta ctgttgctgt atggattaac aggagtgtaa tcaaaatacg
    21661 ccgattttgc acctgttccg tgttagcgcg tcttaataac cagacgatta ctctgcttga
    21721 cttcttccat aacaacgtat gtccgtgtgt cattgacgcc aggcagacgc agcagggttt
    21781 cccccagcaa cttacggtaa gctgacatat ccggcacgcg tgttttcaac aggtagtcga
    21841 aatcaccaga tactaaatga cactcctgaa tttcttcaag tttttgtaca gcggtattga
    21901 attgttcaaa cacatccggt gcgccacgat tcagagtaat ctcaacgaat accagaagtg
    21961 atgcatccag ataatggggg ttcagcagcg ccgtatagcc ctgaataaac ccttgtcttt
    22021 ccagccgacg cacacgctca aggcacggcg ttggggaaag tcccacacgt ttagaaagct
    22081 cgacgttaga aatacgccca tccttttgca actcattaag aatgttacga tcgatacggt
    22141 cgagatcttt gccagggcgc ttcttgctat ctaccattat tattgtctct ctgtattcct
    22201 tccctactcc tgtctgaccc tgaaacatca ctgttcagag tcgctacccg tcacgataga
    22261 ccgaaatcat gaagattcac ggcgcgcaat gcctgggtct acggtgagaa gaccgttgcc
    22321 ttgtggcagc tatcgacatc acgaatggcg tctgtccacg ccatacgtag atttcactag
    22381 cacggtaaac atggtattta catgcatggt tatgcgcata taagaatact gtttttttct
    22441 tcctcgaatg ttttcgcaaa acaccagggg attgtcaaag caaaacatcg atttttagta
    22501 caacatgcca gatattcatt aacaggggcg tttattcatc atttaatcgg cttataaagc
    22561 gggaaattta agtagcagag gtgataatga cgggagaatt tgacctgctt atcgatgctg
    22621 ccgattgcac aaattgttaa caaaatcgtc atactctttt tttacgtctg taaattccct
    22681 acaatcctgc ccattgtctg ccaacaacta tggggatctc atgggcacga ccaaacacag
    22741 taaactgctt atcctgggtt caggcccggc gggatacacc gctgctgtct acgcggcgcg
    22801 cgccaacctg caacctgtgc tgattaccgg catggaaaaa ggcggccaac tgaccaccac
    22861 cacggaagtg gaaaactggc ctggcgatcc aaacgatctg accggtccgt tattaatgga
    22921 gcgcatgcac gaacatgcca ccaagtttga aactgagatc atttttgatc atatcaacaa
    22981 ggtggatctg caaaaccgtc cgttccgtct gaatggcgat aacggcgaat acacttgcga
    23041 cgcgctgatt attgccaccg gagcttctgc acgctatctc ggcctgccct ctgaagaagc
    23101 ctttaaaggc agtggggttt ctgcttgtgc aacctgcgac ggtttcttct atcgcaacca
    23161 gaaagttgcg gtcatcggcg gcggcaatac cgcggttgaa gaggcgctgt atctgtctaa
    23221 catcgcttcg gaagtgcatc ttattcaccg ccgtgacggt ttccgcgcgg aaaaaatcct
    23281 tattaagcgt ctgatggata aagtggagaa cggcaacatc attctgcaca ccaaccgtac
    23341 gctggaagaa gtgaccggcg atcaaatggg tgtcactggc gttcgtctgc gcgatacgca
    23401 aaacagcgat aacatcgagt cactcgacgt tgctggtctg tttgttgcta tcggtcacag
    23461 cccgaatact gccattttcg aagggcagct ggaactggaa aacggctaca tcaaagtgca
    23521 gtcgggtatt catggtaatg ccacccagac cagcattcct ggcgtctttg ccgcgggtga
    23581 cgtgatggat catatttatc gtcaggccat tacttcggcc ggtacaggct gcatggcagc
    23641 acttgatgcg gaacgctacc tcgatggttt agctgacgca aaataatttt acaaatcagt
    23701 aacaaaagta aagaaggcga caccatgcga ctatgggtcg cctttatttt ttccccgttg
    23761 taacattgct ctgcaaataa ttctgataac tcacctgcta agcgtgcaat gaataaatcc
    23821 cgtcaaaaag agttaacccg ctggttaaaa cagcaaagcg tcatctccca acgttggctg
    23881 aatatttctc gtctgctggg ctttgtaagc ggcatattga tcattgccca ggcctggttc
    23941 atggcgcgaa ttctgcaaca tatgattatg gagaatattc cccgtgaagc cctgctgctt
    24001 ccctttacgt tactggttct gacctttgta ctgcgcgcat gggtggtctg gttacgcgaa
    24061 cgggtgggtt atcacgccgg gcagcatatc cgctttgcca tccgccgtca ggttctcgac
    24121 cgtctgcaac aagcagggcc agcgtggatt cagggtaaac ctgcggggag ctgggcgacg
    24181 ttggtactcg agcaaattga cgatatgcat gattactatg cacgctacct gccgcaaatg
    24241 gcgctggcag tgtcggtgcc gttgctgatt gtggtggcga tcttcccctc taactgggct
    24301 gcggcgctca ttctgctggg cacagcaccg ttaattccgt tgtttatggc gctggttggg
    24361 atgggggctg ccgatgctaa ccggcgtaac tttctcgctc ttgctcgctt aagtgggcat
    24421 ttcctcgatc gcctgcgcgg catggaaaca ttgcgtattt ttggtcgtgg tgaagctgaa
    24481 attgaaagta ttcgttctgc ttcggaagat ttccgccaac ggacaatgga agtgctacgg
    24541 ctggcgtttt tatcctccgg cattctcgaa ttttttacct cgctgtcaat tgctctggtg
    24601 gcggtctact ttggtttttc ctatctcggc gagctggatt ttggtcacta cgataccggt
    24661 gtgacgctgg ctgcgggttt tctggccctg atccttgcgc cagagttttt ccagccattg
    24721 cgcgatctcg gtacgtttta tcatgctaaa gcccaggctg ttggtgcagc tgacagtctg
    24781 aaaacgttta tggaaacccc gctcgcccat ccgcagcgtg gcgaggtgga attagcatcg
    24841 accgatccgg tgaccattga ggccgaggat ctgtttatca cgtcgccgga aggtaaaacg
    24901 ctggccggac cgctgaactt tactttgcca gcaggccaac gtgcagtgtt ggttggtcgc
    24961 agcggttcag gtaaaagctc actgctgaac gcgctttctg gttttctctc atatcaggga
    25021 tcattacgaa tcaacgggat agaattacgc gatttatcac cagaatcatg gcgtaaacat
    25081 ctctcctggg ttgggcaaaa cccacaatta ccggcagcaa cattgcggga taacgtacta
    25141 ctggcgcgac ctgatgccag cgaacaagaa ttacaagcag cgctggataa cgcctgggtc
    25201 agcgagtttc taccgctcct gccacaaggc gttgatacac ctgttggcga ccaggctgcc
    25261 cgcctttccg tggggcaggc gcagcgcgtg gcggtggccc gtgcgttact aaatccctgt
    25321 tcgctattac tgttggatga acccgctgcc agccttgatg ctcacagtga acagcgcgta
    25381 atggaggcgc tgaatgccgc ctctctgcgc cagacaacgt taatggtcac ccaccagtta
    25441 gaagatcttg ctgactggga tgtcatttgg gttatgcagg atggccggat tattgagcaa
    25501 ggacgttacg cggaattaag tgtggctggt ggcccattcg ccacattact ggcccatcgt
    25561 caggaggaga tttaaatgcg cgctttgcta ccctatctgg cactgtataa acgtcataaa
    25621 tggatgttaa gtcttggtat cgtgctggca attgtcacgc tgctcgccag tatcggtctg
    25681 ttgacacttt ccggctggtt cctctcggcc tcagcggttg cgggggttgc cggactgtac
    25741 agcttcaact atatgctacc cgctgcgggc gtgcgtggcg cagcaatcac ccgtactgcc
    25801 gggcgttatt ttgaacgtct ggtaagtcac gacgcgactt tccgcgtgtt gcagcatctg
    25861 cgcatttaca ccttcagcaa attgctgccc ctctcccctg ccggactggc gcgctatcgt
    25921 cagggcgaat tgctcaatcg cgtggtggcg gatgttgata cgctcgatca tctttatctg
    25981 cgcgttatct cgccgctggt gggcgctttt gtggtgatta tggtggtgac aatcgggtta
    26041 agtttccttg atttcaccct cgcctttagg ctgggcggca ttatgttact gacgcttttc
    26101 ctgatgccac cgctgtttta tcgtgcggga aaaagcaccg ggcaaaatct gactcatctt
    26161 cgcggacagt atcgccaaca actgacggcc tggctgcaag ggcaagctga gctgaccatt
    26221 tttggcgcca gcgatcgtta tcgcacgcaa ctagagaata cagaaattca atggctggaa
    26281 gcgcaacgcc gtcaatctga actgaccgca ttgtcgcaag cgataatgct gctcattggc
    26341 gcgttagcgg tgatcctgat gctgtggatg gcgtctggcg gcgttggcgg caatgctcaa
    26401 cccggcgcgt taattgccct gtttgtcttc tgcgcgttag ccgcgtttga agcactggca
    26461 ccagtaacgg gtgcatttca gcatctgggg caagtcattg cctctgccgt tcggatcact
    26521 gatttaacgg atcaaaaacc ggaagtcacc ttccctgata cccaaactcg tgttgccgat
    26581 cgcgtttcgc tgacattacg ggatgttcag ttcacttatc cggagcaatc ccaacaggca
    26641 cttaaaggga tttctcttca ggtaaacgcc ggggaacata ttgcgattct cgggcgaacc
    26701 ggatgcggca aatcaacact gttacaactg ctgacccgcg cttgggaccc gcaacaggga
    26761 gagattttgc ttaacgatag ccccatagcc agcctgaatg aagcggctct acgacagacc
    26821 atcagcgttg ttcctcagcg agtgcatctg tttagcgcca cgctgcgtga taatctgtta
    26881 ctcgcctcgc ctggcagtag tgatgaggct ctggcggaga gcttgcgtcg cgttggtctg
    26941 gagaagctgc tcgaggatgc aggtctcaac agttggttag gtgaaggcgg acgccagctc
    27001 tccggtggtg aactgcgccg tctggctatc gcccgtgcgc tgttacatga tgcgccactg
    27061 gtgttgctgg atgaacctac cgaaggctta gatgccacaa ccgaaagcca gatccttgaa
    27121 ttgcttgcag aaatgatgcg tgagaaaacg gtgttaatgg tcacccatcg acttcgcgga
    27181 ctctctcgtt tccaacaaat aatagtgatg gacaacgggc aaattattga gcaaggtact
    27241 cacgcagagc tgcttgccag acaggggcgt tattaccagt tcaagcaggg tttgtaagct
    27301 attattgaac gatccgactt gcgtggagtt ttgcggtcat gcgcctggtt cagctttctc
    27361 gccattcaat agccttccct tccccggaag gcgcattacg tgagcctaac ggcctgctgg
    27421 cacttggggg cgatcttagc cctgcgcgcc tgttaatggc ttaccagcgt ggtatttttc
    27481 cgtggttttc tccaggcgac cccatcctct ggtggtcgcc cgatccccgc gcggtgctat
    27541 ggccagaatc actgcatatc agccgtagta tgaagcgatt tcataaacgc tcgccctatc
    27601 gtgtcacgat gaattacgct tttggtcagg tcattgaagg ctgtgccagc gatcgcgaag
    27661 aaggaacctg gatcacgcgt ggcgtggtcg aagcctacca tcgccttcac gaactcggcc
    27721 atgcccactc cattgaagtc tggcgtgaag atgagcttgt cggcggtatg tacggcgtgg
    27781 cccagggaac gctattttgt ggcgagtcca tgttcagccg gatggaaaat gcgtctaaaa
    27841 cggcgcttct ggtattctgt gaggaattta tcggtcatgg cggtaagctt atcgactgcc
    27901 aggtccttaa cgatcacaca gcatcgcttg gtgcctgcga aattccccgc cgggattacc
    27961 ttaattatct caatcaaatg cgcctcggac gattgccgaa taatttctgg gtaccacgat
    28021 gcttgttttc accacaagaa tgaatgtttt cggcacattt ctccccagag tgttataatt
    28081 gcggtcgcag agttggttac gctcattacc ccgctgccga taaggatttt ttcgcgtcag
    28141 gtaacgccca tcgtttatct caccgctccc ttatacgttg cgcttttggt gcggcttagc
    28201 cgtgtgtttt cggagtaatg tgccgaacct gtttgttgcg atttagcgcg caaatcttta
    28261 cttatttaca gaacttcggc attatcttgc cggttcaaat tacggtagtg ataccccaga
    28321 ggattagatg gccaaagaag acaatattga aatgcaaggt accgttcttg aaacgttgcc
    28381 taataccatg ttccgcgtag agttagaaaa cggtcacgtg gttactgcac acatctccgg
    28441 taaaatgcgc aaaaactaca tccgcatcct gacgggcgac aaagtgactg ttgaactgac
    28501 cccgtacgac ctgagcaaag gccgcattgt cttccgtagt cgctgattgt tttaccgcct
    28561 gatgggcgaa gagaaagaac gagtaaaagg tcggtttaac cggccttttt attttgtgat
    28621 atgtatgaag tactttggaa gtataagtcc gtaacttgtc tcgatgtaga tatactgcac
    28681 aacttgttta cagtatgagc aaacaataat tatcattaaa attcttcttg accgttttac
    28741 tccaactccc tatagtagcg ccccgttgcc acccatgagg tttggtagca aaacaatttg
    28801 gtgaggtgtc cgagtggctg aaggagcacg cctggaaagt gtgtatacgg caacgtatcg
    28861 ggggttcgaa tccccccctc accgccagat tacaagcgca tgttattgtt taatatgcgc
    28921 tttttttact tatccttcca gacagtatgc caccgggtat gccatctgaa accggtttgt
    28981 atgagccgcg atcacatctt atcggacaat ttccgatctt tgagcatact ggcaaagcta
    29041 tactgacagc atgtcaggat agtgttctgc aataatatca ttcagtcggt caaccagttc
    29101 cggttttctg aacgtgatat gcgcggagcc cttcctaaag tatctgatgc tgaacatctc
    29161 atcttcatag ctctcttgac cctgaacaga ctgaatgtga tcatccagac gaatagtgat
    29221 gttttcccgg ttgtcgggga ttggtttacc gctgaacagg tgcaacattc tttccagatc
    29281 ggcgagtcgg tcagcctgtt gtccggtgat aagatgaaat ctatggtcac ccccgttttt
    29341 gcaacactga tttttgatta atggttggct tgcttaaatc tatccggcgt ctctgtgggc
    29401 aaggatgccc gcgccccgat gagttccgca cctgataatc ctaaaaatcc cctcggcttc
    29461 agtgagccat ttcatctgtt aggtttttaa tcaggtcgat ttgccgttca tgtcatcaat
    29521 aatcagtctt cgcaaaacca gaaaggtaac ttgtatctta ttgtttaaaa taaactaatt
    29581 gagttatatc agcatctata tcaggcctga tattcttttt tctgagtagt gattgcccag
    29641 gcaattcgag ccagtttatt tgccagagcg caggtaacca cattcgaatg cttcttgttt
    29701 agttgctccc gaacccactc agcaagtttt ccatgcttgc gttcaagtcg cattatgaat
    29761 gagcgggcac actgaaccag taaccggcgt aaatttttat cacctcgctt actgataccc
    29821 aacagagtcg attttcctcc agtgctatac tgccggggaa caagtcctgt ggaagctgca
    29881 aaatccctgc tgcatgagaa ctgtttcccg tcaccaagtt gcgacgatag taagctggca
    29941 gtaatcggac caacaccagg aattgtcata atgcgctgag cggtatcatc agcgttgatt
    30001 gactgactta attctgattc cagctctgct atttgctcaa caaggtaaaa gtagtgggta
    30061 tgcaacctca ccaaaaggcg gctcagatat tcggggattt cgtgttcagc caacacgaga
    30121 gacaaccgtt ttattacagc atcacctatc ggcaggctga ttccaaattc cagcaagaaa
    30181 ccgtggatct ggttggttgt tttcacctta tctctgatga gcgattctct gacccggtgt
    30241 aacgccctca tggcctgctg ggtctcagtt cttggctgga caaaacgcat tgatggtcgt
    30301 gaagccgctt cgcatatggc ttctgcatca acaaaatcat tcttattact tttgacaaac
    30361 gggcgaacga actgtggaga aatcagctta gtttcatgcc caaaatcaga gatacagcga
    30421 gccataaagt gtgcacccgc acaggcttcc attacaacaa ctgagggcgg acatgtcgcc
    30481 agaaactgca tcagtttagt acgggtaaat tttttacgca gaagagcctt accagacttg
    30541 tcttgacaat gaatgtggaa ggagtgcttt ccgagatcga taccaataag tgtgatgttc
    30601 tgcatgatgg tctctccgtt aaagggattt accttaacag cgtactgctg ttaaggtggg
    30661 ggtgaccata tcattatccc caccggtccc atctcaccag attgttgacg ataattttac
    30721 tgccaaattt gcaggggcaa ttggttttgt aattccagct cagtcccctg aaaacattga
    30781 tcacccctcg ctcaaacacc tcatccttat tctggtgcag ttgttcaaat gtgctcagaa
    30841 tgttcgcttc actgatttcc ggaaagttat cgtactccag tgaccggtac catgtatcac
    30901 gggcctgtgc atccattaag gaaagcatcc ctgaacgttg catcaggtca cgccagattt
    30961 cgcgatcgag attgcgggta atggctttca ttgccgtttc cggtttctcc atcagccagc
    31021 agccgcagcg aaaatcctgt ttcatggccc agtccagggc agttttacct ccgatgcttt
    31081 ttgtcatagc tgagatttct gccagttgtc ggataagtgt gtcgatttgc agcagtgtac
    31141 tctttcgtac agccagaatg tggcggatac tggttgagca gataacgtcc gtatgcccgg
    31201 tgaccacttc cggacgggag attgtgatgt ctgacattat cggtttccct tttggtttat
    31261 aaacgaaaac gccagccctg ttcaggctga cgtcagtgga atgaagtctg gtgggtaaga
    31321 ctcaccggtt ctgttgcaga agctgcaact ggcgcagggt atggagatct tccctggcct
    31381 gttcaatgca gggcagcagt gctgccgaat ccgctttatc ctcatcgtta tcaaagataa
//
LOCUS       DAZGJW010000048        30997 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZGJW010000048 DAZGJW010000000
VERSION     DAZGJW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.048"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHKIK_002159"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHKIK_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO9083325.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHKIK_002160"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHKIK_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO9083326.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHKIK_002161"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHKIK_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO9083327.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHKIK_002162"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHKIK_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO9083328.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHKIK_002163"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHKIK_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO9083329.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHKIK_002164"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHKIK_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO9083330.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHKIK_002165"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHKIK_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO9083331.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHKIK_002166"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHKIK_002166"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO9083332.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHKIK_002167"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHKIK_002167"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO9083333.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHKIK_002168"
     CDS             12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHKIK_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HFO9083334.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHKIK_002169"
     CDS             complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHKIK_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO9083335.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHKIK_002170"
     CDS             complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHKIK_002170"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO9083336.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHKIK_002171"
     CDS             complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHKIK_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO9083337.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHKIK_002172"
     CDS             complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHKIK_002172"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO9083338.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHKIK_002173"
     CDS             complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHKIK_002173"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO9083339.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHKIK_002174"
     CDS             complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHKIK_002174"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO9083340.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19283..19383)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHKIK_002175"
     CDS             complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHKIK_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO9083341.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20006..20779
                     /gene="nudC"
                     /locus_tag="ACHKIK_002176"
     CDS             20006..20779
                     /gene="nudC"
                     /locus_tag="ACHKIK_002176"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO9083342.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20819..21883
                     /gene="hemE"
                     /locus_tag="ACHKIK_002177"
     CDS             20819..21883
                     /gene="hemE"
                     /locus_tag="ACHKIK_002177"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO9083343.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21893..22564
                     /gene="nfi"
                     /locus_tag="ACHKIK_002178"
     CDS             21893..22564
                     /gene="nfi"
                     /locus_tag="ACHKIK_002178"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO9083344.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHKIK_002179"
     CDS             22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHKIK_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO9083345.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGDSNIGIIFQQ"
     gene            23384..23656
                     /gene="hupA"
                     /locus_tag="ACHKIK_002180"
     CDS             23384..23656
                     /gene="hupA"
                     /locus_tag="ACHKIK_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO9083346.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHKIK_002181"
     CDS             23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHKIK_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO9083347.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRAAQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACHKIK_002182"
     CDS             complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACHKIK_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO9083348.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            complement(24794..24922)
                     /locus_tag="ACHKIK_002183"
     CDS             complement(24794..24922)
                     /locus_tag="ACHKIK_002183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083349.1"
                     /translation="MTRYPQQASHFYSFIMPDYPSYQRFIVGTEDAIPEVRQPLED"
     gene            25029..26405
                     /gene="zraS"
                     /locus_tag="ACHKIK_002184"
     CDS             25029..26405
                     /gene="zraS"
                     /locus_tag="ACHKIK_002184"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO9083350.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVGQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26402..27727
                     /gene="zraR"
                     /locus_tag="ACHKIK_002185"
     CDS             26402..27727
                     /gene="zraR"
                     /locus_tag="ACHKIK_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO9083351.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDTLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCVALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLASHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERSVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTCGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACHKIK_002186"
     CDS             complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACHKIK_002186"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO9083352.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACHKIK_002187"
     CDS             complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACHKIK_002187"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO9083353.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7302 a         8042 c         8483 g         7170 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcat tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagacccc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacac cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga ccgacggtgt tgtaactgac gaaattcact acctgtctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgacagacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gaggggggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaagtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaata ctgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtaccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 tcagaccatt acgcgtcaga cagacgaatt gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga ttccgggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gcacgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtta ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaaggaat ttctgaatac
    12781 tcataatcaa tgtagagatg actaatatcc tgaaactgac tgaactaatt gagtcaaact
    12841 cggcaaggat tcgatactat tcctgtgtaa ctttcttaag gaacgagaat gaaacaggaa
    12901 gtggaaaagt ggcgaccttt tggacatccg gatggtgata ttcgtgattt atcatttctt
    12961 gatgctcatc aggctgtcta cgttcagcat catgagggca aagagccttt agagtatcgc
    13021 ttttgggtta cctactctct tcactgcttc acaaaagatt atgaacatca gacgaacgaa
    13081 gaaaaacaat cgttaatgta ccacgcgcct aaagaatctc gtcccttctg ccagcaccgt
    13141 tataacttag cgcgcacaca cttaaaaaga actattttgg cgctgccaga aagcaacgtt
    13201 attcatgccg ggtatggtag ctatgccgtg attgaggtgg acttagacgg aggagataag
    13261 gcattttact ttgttgcgtt cagggctttc agggaaaaga aaaaactccg tttgcatgta
    13321 actagcgctt atcccatttc tgaaaaacag aaaggtaaat cagtgaaatt tttcaccatt
    13381 gcctacaact tattgagaaa taagcagctt cctcagccct caaaataaca aaacccacct
    13441 taaggtgggt ttcgccagag aattatctct ggtattcaga acgccattac cggactttgc
    13501 cttgaccttg cgataatcgc aggttgcggg atgtctgaat ttcttcagtc tgctgcatcc
    13561 tggaagatga gaacatgtgt tcttattttc gtctctatca tagttgagta tttactctct
    13621 tacaatcaga tctctttcat tgctcaacag gcgatggctt cagactttgc attacggaat
    13681 ttttaagaaa ggcagggcga aacgaggaag aagcttttcg ccctggacaa cattcctgct
    13741 gacggcacta ccataaaaaa acacaacgct ttcaacaacc ttgcgttttt catcttccgt
    13801 agcggctcgg aataaattta cgttcttaca ttccgtctca tagtctttgc gaggcccgtc
    13861 ccagatagct gtcccagtct ttccataccg gctgcaaacc ctgagcggtt aacgcggcag
    13921 caaccgcttc cggtctgcga tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat
    13981 cggcatagcc acctggctgc gttttcgaga aggcgctgac gttattgatc gccagcggaa
    14041 taacgcgatc gcgaaaccac ggtgattccc gcgtggagag tgacagttca atctccggtg
    14101 caagcagtcg gaaggcgcag atggtttgca ctaactggcg ttcatccata atcgacgcag
    14161 gctcaatgcc gccagtacac gggcgcaggc gcggaaagga gacagagtaa cggctttgcc
    14221 agtaatgctg ttgcagccat agcaaatgtt cggcaaccat atagcagtca acgcgccagt
    14281 tgtcggaaag gccaattagc gcgccgaggc ctatcttatc aatccccgca cgccccagcc
    14341 gatccggcgt ttccagccgc cagaagaagt cctgtttttt tcctttcaga tgatggcggg
    14401 cataagtcgc ctcgtgatat gtctcctgat aaaccatcac gccatccaga ccaagctgct
    14461 ttaactcggc gtattccgtc tccgccagcg gttgcacttc catttgtagt gaagagaact
    14521 gttcacgcag ggcggggaga tgacgacgaa agtaatccat ccccactttc gcctgatgtt
    14581 caccagtgac taatagcaga tgttcaaagc ccatctcccg tatagcggcg ctttccctgg
    14641 caatatccgc ttcatccagc gttttgcgct tgatgcgatt actcatggaa aatccgcagt
    14701 acgtgcagtc gttagcgcaa agattggaaa gataaagcgg gacgtagaaa ctaactgtgt
    14761 tgccaaatcg ctgacgggtc agacgctgcg cccgttgggc cagttgttcc agatagccac
    14821 tggcggctgg cgataacagc gccatcatat cgtcgcgggt gagttgcgag gcatttagcg
    14881 cccgttctac gtcagcagcc gttttgccgt tgatacgcag gcggatgtcg tcccagtcca
    14941 gttgtcgcca gcgatcgctg aaggttttca tgccgatgcc tccagaaatc cagtcagcgg
    15001 gctggtggca tgagcaaaat gactgcggct gcccggtccg gactgacgtg ccagcaggcc
    15061 tgcttctacc gccagacgaa atgccttcgc catgttgacg ggatcgtccg cgacggcaat
    15121 tgccgtattc actaacaccg cgtcggcccc catttccagc gcctgcgcgg catggctggg
    15181 aacgccgatg ccagcatcga caaccaccgg cactgtggcc tgctggataa taatctccag
    15241 catggcgcgg gtttccagtc cctgattcga gccaatcggc gcgccgagcg gcatcaccgc
    15301 tgcacagccg acttcttcca gacgtttaca caataccgga tcggccccgc agtaaggcag
    15361 cacgacaaat ccctgttgta ccagcgtttc ggcggctttc agggtttcga tgggatcggg
    15421 caacagccag cgggcgtcag ggtgaatctc taattttaac cagtttgtgc ctaacgcttc
    15481 acgagccaga tgggcggcga aaatggcttc ttccgctgtt ttcgccccgg atgtatttgg
    15541 cagcagggtc acacccgccg cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa
    15601 gtcgacacgt ttcatcgcca gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac
    15661 catcagttgt gaagaagcga atttccctgt gccggtaaac agatgtgaat caaacgtttt
    15721 gtccgcaata cgtaacattt caaccccctg caataacctg aaaaagcagg atctggtcgc
    15781 catcctgcac gatatgttgc gcccactgct cacgcgggac gatttgctga ttaatcgcca
    15841 gagccgcgcc cgcttgtcgt tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg
    15901 cggcgcactg catcggttga tcgttaaaca ggatctgcat tgcttcctcc gcataccggg
    15961 caaccactgg cgcggcgcaa cgccaggctg cgccactggc tcgatttacc gtcgaacagt
    16021 cggagttctc ccgcaggtgt ctctataccg cttaataact taatggcttc cagtgcctgc
    16081 aaagtgccca taaccccgac caccgggcca accacgcccg ccgtgcggca gttgcgttct
    16141 ggctcctggt tatctggcca caggcagcgg taacacccct gctcccaggg cggcgtcagt
    16201 accatcaact gaccgccaaa tccgaccgcg ctggcggtga taagcggcgt gttgagtgcc
    16261 acgcaggcgg cattaatctc ctggcgagtc gccatattgt cggtacagtc gagcaccaca
    16321 tcggcccgtg caaccgcatc ttttaacgcc tcacccgtta accgttgttg taatgctgtc
    16381 agttgaatgt cgggattcaa ctgtgtcagt cgctgttggc tgacctgcga tttcgggcga
    16441 tcgatatctt cagtggtaaa gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg
    16501 tcatctgcca gtaccagcgt cccgacgcca gcgcccgcca ggtacagcgc agcaggtgta
    16561 cccagcccgc ccagaccgat aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc
    16621 agagcgatat cgtcgagcag gatttggcgg ctataacgca taaagtcacg gtcattcatc
    16681 gccaactcct gcaatttcca gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt
    16741 aatggcgctg acgacggcga tactgccgac acccgttgct atcaccgcag gcgcgcgtgc
    16801 cagactgata ccgccaatcg ccacggtggg ataatccgcc agtcgctcaa catgccgtgc
    16861 cagctgttcc agcccctgcg gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg
    16921 tcccagcgcg atataagagg ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg
    16981 tgtcgaaacg cccagccgca ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa
    17041 atcttcctgc cccaaatgga cgccatacgc ctgatgcttg atcgccagcc gccagtaatc
    17101 gttgataaac aatcgcgcgt tatagcggcg gcccagcgca attgccgcca cgacatcggc
    17161 ttccacctct tcatcgcgcc gatctttgat gcgtagctgg agagtacgta cgcctgcatc
    17221 caacagacgt tcgatccact gtacgctgtc caccaccggg tacagtccta aacgaaaagg
    17281 tacaggagga aaatcaggct gatacatcac gcttcctcct tacgcaggta gatttctccg
    17341 cctctggcac ggaagttctc cgacatatcc gccattccca tttcaatagt ttgcgtggcg
    17401 gcgtaatcac gcacttcctg gctgattttc atcgagcaaa atttcggtcc acacatggag
    17461 caaaaatggg cgactttgcc ggactcttgg ggcagggttt catcgtgata agcgcgggcg
    17521 gtaaacgggt cgagggccag attaaactgg tcttcccagc gaaattcgaa gcgggctttc
    17581 gacatggcgt tatcgcgaat ttgcgcgccc ggatgccctt tcgccaggtc agcggcgtgg
    17641 gcggcaatct tataggtgat aagcccctgc ttaacatctt ctttattagg cagacccaga
    17701 tgctcttttg gcgttacgta acagagcatc gcgcagccaa accagccaat catcgccgca
    17761 ccaatccccg acgtgaagtg gtcatagccc ggcgcaatat cggtagttag cggccccaga
    17821 gtgtaaaacg gcgcttcgtg gcagtgctct aactcctcgg tcatattgcg gcggatcatc
    17881 tgcatcggca cgtggcctgg gccttcaatc atcacctgca cgtcatattc ccaggcaatt
    17941 ttggtcagtt cgcccagcgt atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga
    18001 atagaaccgg ggcgcagacc gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt
    18061 tcacaaattt cgcggaagtg ttgatagagg aaattttcct gatgatggga gaggcaccat
    18121 ttcgccataa tcgaaccgcc gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc
    18181 acatagcgca gcagtacgcc cgcatggata gtgaagtaat ccacaccttg ctcggcctgt
    18241 tccagcagcg tgtcgcggaa cgcttcccag gtaagatctt cggcgatccc gttaaccttc
    18301 tccagcgcct ggtagatcgg cactgtaccg atcggcaccg ggctgttacg caaaatccac
    18361 tcgcgggttt cgtgaatata gcgaccggtg gagagatcca tcaccgtatc cgctccccag
    18421 cgcgtggacc ataccagctt ttccacttct tcttcgatgg aagaggtgac cgcagagttg
    18481 ccgatattgg cgttaacttt taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc
    18541 ggatgattaa tgttggccgg gataatcgca cgtccggcag caacttcatc acggacaaat
    18601 tccgcagtga tattttccgg cagacgtgcg ccaaagctca ttcccggatg ctggtggcgt
    18661 aaaacttcgc tacggatgcg ctcgcggccc atattctcgc ggatggcgat gaattccatt
    18721 tccggtgtga tgatgccctg gcgggcgtag tgcagttggg tgacgcggcg tcctgctttg
    18781 gcgcgttttg gtgttagtac gccgctaaaa cgcagttcgt cgaggccatc atctgccagc
    18841 cgcgctttag tgtaatcgga actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg
    18901 atccacggct ggcgtagttt tgccagccct tgctgcacgt taatggcaat ctgcggatca
    18961 ccatacgggc cggaggtgtc gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt
    19021 tctttgctac cgccaattag cgtcgggcta agctggatct cacgcatcgg cacgcgcacg
    19081 ccggggtgtg tgccagtgat ataaatgcgt tttgagttgg gaaaggcggt gccttccagg
    19141 gtgtcgataa aatgttgggc ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac
    19201 atagctcatt ccaaaaagtt aaggacgtgg cttgtcagac gacggatgaa gcaagagacg
    19261 atcgccgcag gggcgatgcg atagcagatt aactcttgtt cccttcgcag gtattagcct
    19321 gatcaggttc cgcggatccc gaataaacgg tctcagccag ttaaggcact ccgacaagaa
    19381 attagccccg caaatggggc attgaatgta aattacgcgt taacagcgca gaactcaagc
    19441 aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc agcttatctt ccagcacaaa
    19501 gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc
    19561 atgatcgatg gcggtttcca gactggaatc gtagtaatcc ataatctgtt gagtattggc
    19621 ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc ccgttgcctt ccagcttatg
    19681 aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag taatcgacca ggctctgaca
    19741 aaaatcatca agggcttttt cgtttagcct catgtacgat tctttgccag gcttaatgcc
    19801 aaccagattg tagtaagcca cgagcagatg cttacgtaca tgtagccagc gatcaaccag
    19861 tttgttactt cctctgacgc gctccgtcag gttatcgagc tggttaagca tgattgactc
    19921 cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca
    19981 gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt gaaaaattag atcacggctg
    20041 gtgggtcgtc agccatgaac aaaaattatg gttgccgaag ggagaattgc catatggcga
    20101 agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa atcggcgaat ggcaggggga
    20161 acctgtttgg ttagtacaac agcagcggcg tcacgatatg gggtcggtac gtcaggtcat
    20221 tgatctcgat gttgggctgt ttcaactggc cggacgaggc gtacaactgg cggagtttta
    20281 ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg tatccgagca aaaccgaatg
    20341 ggcgatgctg tgcagccatt gccgtgagcg ttactacccg caaatcgccc cctgcattat
    20401 tgttgctatc cgtcgcgatg attcgatcct cctcgcccag catacccgcc atcgtaacgg
    20461 tgtccataca gtacttgccg gattcgtcga agtgggcgaa accctcgagc aggcagtcgc
    20521 gcgggaagtg atggaagaga gcggaattaa agttaaaaac ttgcgttacg tgacttctca
    20581 gccgtggccg tttcctcagt ctttaatgac cgcgtttatg gcggaatatg acagcggcga
    20641 catcgtgatc gacccgaaag aattgctcga ggcgaactgg tatcgctatg acgatttgcc
    20701 gttactcccg ccgcccggca ccgtagcgcg ccgtctgata gaagatacgg tggcgatgtg
    20761 tcgggcagag tatgagtgat gatacactga ccgcctgacg cactaaggaa cagccaaaat
    20821 gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac
    20881 tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc
    20941 ccaggcaggc gattttatgt cgctgtgcaa aaacgccgag ctggcgtgcg aagtgacttt
    21001 gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc ttttccgata tcctcaccgt
    21061 gccggacgcg atggggttag ggctctattt tgaagccgga gaaggtccgc gttttacctc
    21121 gccagtcacc tgcaaagctg acgtcgataa actgccaatt ccggacccgg aagatgaact
    21181 gggttacgtg atgaacgcgg tgcgtaccat tcgtcgcgaa ctgaaaggcg aagtgccgct
    21241 gattggtttt tccggcagcc cgtggacgct ggcgacctac atggtggaag gcggcagcag
    21301 caaagccttc accgtgatca aaaaaatgat gtatgccgat ccgcaggcgc tgcacgctct
    21361 gctcgataaa ctggcgaaaa gcgtcacttt gtatctgaat gcgcagatta aagccggtgc
    21421 tcaggctgtg atgattttcg acacctgggg cggcgtgctt accgggcgcg attatcaaca
    21481 gttctcgctc tattacatgc ataaaattgt tgatggttta ctgcgtgaaa acgacggtcg
    21541 ccgcgtgcca gtcacgctgt ttaccaaagg cggcggacag tggctggaag cgatggcaga
    21601 aaccggttgc gatgcgctgg gcctcgactg gacaacggat atcgccgatg cgcgccgccg
    21661 cgtgggcaat aaagtcgcgt tgcagggcaa tatggatccg tcgatgctgt acgcgccgcc
    21721 agcccgcatt gaagaagaag tagcgactat acttgcaggt ttcggtcacg gcgaaggtca
    21781 tgtctttaac cttggtcacg gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt
    21841 cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc taaggagtga ttatggatct
    21901 cgcgtcatta cgcgctcaac aaatcgaact ggcttcttct gtgatccgcg aggatcgact
    21961 cgataaagat ccaccggatc tgatcgccgg agccgatgtc gggtttgagc agggcggaga
    22021 agtgacgcga gcggcgatgg tgctgctgaa atatccctcg cttgagctgg tggagtataa
    22081 agtggcccgc atcgccacca ccatgcctta cattccaggt tttctttcct tccgcgaata
    22141 tcctgcgctg ctggcagcgt gggagatgct gtcgcaaaag ccggatttag tgtttgtcga
    22201 tggtcatggg atctcgcatc cccgccgtct tggcgtcgcc agccattttg gcttattggt
    22261 ggatgtgccg accattggcg tggcgaaaaa acggctctgc ggtaaattcg aaccgctctc
    22321 cagcgaaccg ggcgcgctgg ccccactgat ggataaaggc gagcagctgg cctgggtctg
    22381 gcgcagcaaa gcgcgctgta acccgttgtt tatcgctacc ggccatcggg tcagcgtgga
    22441 cagcgcgctg gcgtgggtac aacgctgcat gaaaggctat cgtctgccgg agccaacgcg
    22501 ctgggcggac gcggtggcct cggaacgtcc ggcgttcgtg cgctatacag caaatcagcc
    22561 ctaattcagg taaactgcgg ataatttccg tatttgagaa ctcaacatgt tacaaaaccc
    22621 aattcatctg cgtctggagc gcctggaaag ctggcagcat gtcactttca tggcttgctt
    22681 atgcgaacgc atgtacccca attacgccat gttctgccag caaaccggtt ttggtgatgg
    22741 gcaaatttac cgtcgcattc tcgatctcat ctgggaaacg ctgaccgtta aagacgcaaa
    22801 agtaaatttc gacagccaac tggagaaatt tgaagaagcg attccttcag ccgacgattt
    22861 cgatctgtac ggcgtttatc cggcaatcga tgcctgcgtg gcgttaagtg aactggtcca
    22921 ttcgcgtttg agtggtgaaa cgctcgaaca cgcggtggaa gtgagtaaga cctccatcac
    22981 gaccgttgcg atgctggaaa tgactcaggc tggtcgcgaa atgagcgatg aagagcttaa
    23041 agaaaaccca gctgtagagc aagaatggga cattcagtgg gaaatattcc gacttttagc
    23101 tgagtgcgaa gaacgcgata tcgagctgat aaaaggcctt agggcagacc tgcgtgaggc
    23161 gggtgatagc aatattggta taatttttca gcaataagac cagaaaacgt gatttaacgc
    23221 ctgatttgtc gtacctggag tcttcccttt cgccccccgt ctggtctaca tttggggggc
    23281 gaaaaaaagt ggctatcggt gcgtgtatgc aggagagtgc tattctggca tttccgtcgc
    23341 actcgatgct tagcaagcga taaacacatt gtaaggataa cttatgaaca agactcaact
    23401 gattgatgta attgcagaga aagcagaact gtccaaaacc caggctaaag ctgctctgga
    23461 gtccactctg gctgcaatta ctgagtctct gaaagaaggc gatgctgtac aactggttgg
    23521 tttcggtacc ttcaaagtga accaccgcgc tgagcgtact ggccgcaacc cgcagaccgg
    23581 taaagaaatc aaaattgccg cagctaacgt accggcattt gtttctggca aggcactgaa
    23641 agacgcagtt aagtaagatt gcgtggcagt gaacagtttt aacgaagggg tggtttcacc
    23701 ccttttgtct ttctggcgtc gatcattgat gctggcaggt gcgctgtttc tcactgcctg
    23761 tagccacaac tcttcacttc ctccctttac cgccagtgga tttgctgaag accagggcgc
    23821 ggtacgcatc tggcgaaaag acagcggcga taatgtgcat ctgcttgccg tgtttagccc
    23881 gtggcgcagt ggcgatacca cgacgcgaga gtatcgctgg cagggcgata acctcacgct
    23941 catcaatatc aatgtttaca gcaaaccgcc ggtgaacatt cgcgcgcgtt ttgacgatcg
    24001 cggtgatctg agctttatgc aacgtgaatc cgatggggaa aagcagcagc tttctaacga
    24061 ccaaatcgat ttataccgtt atcgtgctgc tcagatccgc cagattagcg atgccttacg
    24121 tcaggggaga gtcgtgctgc gccaggggcg ctggcatgcg atggaacaga ccgtgaccac
    24181 ctgcgaaggg caaaccatta aacctgattt agattcgcag gcgatagcgc atatcgagcg
    24241 ccgccagagc cgctcttctg ttgatgtcag cgtggcatgg ctggaagcgc ccgaaggttc
    24301 gcaattactg ttagtggcaa actctgattt ctgtcgctgg caacccaacg agaaaacgtt
    24361 ctgatttacc agtggcccat acccatatga ccgccaccac cgcagccgcc gtagcccatg
    24421 cccattccgg ctccgcgcgg aatacccgct tcagccatcg cgatatcccg tttcacccgt
    24481 aactcatcta acgactgacg caaattctcc atctctttgg cgaccgcgtt aattttgctg
    24541 ctatccggtg ggttcgcggc taacagggca ttgtattcat aacgcttcgt caccagttgc
    24601 tgttgcagtg cgctgctttg agcgtaaaag tcattatgga ttttctgcca cgctgtctgt
    24661 tgttcgctgg tcaaaggcgc ggcattttgc tgccacatac cgtgtccgcc gtgagcaaat
    24721 gcagatgtcg atcccatcgc cattgctgaa agcgccatca ttaccagggc aattttcgtg
    24781 ttccgtttca tggttaatcc tccagtggtt gtcttacttc gggtattgca tcttccgtgc
    24841 caacgatgaa acgctgatat gacgggtaat ctggcatgat aaacgagtaa aaatgactcg
    24901 cctgctgcgg gtagcgagtc atttttactc attgaaactt aagcctttgt gttacagcgc
    24961 agggtaagcg ctgataaaag atggcatgat ttctgctgtc agaaagggat gagcaggcaa
    25021 agaagaagat gcgttttatg caacgttcta aagactcctt agctaaatgg ttaagcgcga
    25081 tcctccccgt ggtcattgtt gggctggtag ggttgtttgc ggtgacggtg attcgcgatt
    25141 atgggcgcga gactgccgcc gccagacaaa cgctgctgga aaaaggcagt gtacttattc
    25201 gcgctcttga atccggctcg cgcgtcggca tggggatgcg catgcatcat gcgcagcagc
    25261 aggccttact ggaagaaatg gccgggcagc ctggagtacg ttggtttgcg gtcacggatg
    25321 aacaaggaac aatcgtgatg catagcaact ccggcatggt gggaaaacag ctttattccc
    25381 cgcaggaaat gcagcagtta catccgggag atgaagaagc gtggcggcgg atcgatagcg
    25441 cagacggcga gcctgttctg gaaatttatc gccagtttca accgatgttt gctgctggaa
    25501 tgcaccggat gcgccatatg cagcagtatg ccgcgacacc acaagcaatt ttcatcgctt
    25561 tcgacgccag taatattgtg agtgccgaag atcgtgagca gagaaacacc ctgattatcc
    25621 tcttcgccct ggcgacggtc ttgctggcaa gcgtattgtc attcttctgg tatcgccgct
    25681 atctgcgctc gcgccagctt ctacaagatg aaatgaagcg caaagagaag ctagtggcgc
    25741 tggggcatct tgcggcaggc gttgcccacg aaatccgtaa cccactttcc tcgattaaag
    25801 gactggcgaa atactttgcc gagcgcgcgc ctgcaggggg agaagcgcat caactggcgc
    25861 aggtgatggc gaaagaggcc gaccgtttaa accgcgtggt aagcgagctg ctggaactgg
    25921 ttaagccaac gcatctggct ttgcaggcgg tggatctcaa cacgctgatt aaccactcat
    25981 tacagctggt aggccaggat gcaaacagcc gggagatcca gttacgcttt accgccaacg
    26041 acacattacc ggaaattcag gccgatccgg acaggctgac tcaggtcctg ttgaatctct
    26101 atctcaatgc tattcaggcg attggtcagc atggcgtgat tagcgtgacg gtcagcgaaa
    26161 gcggcgcggg cgtgaaaatc agcgttaccg acagcggtaa gggaattgcg gcagatcagc
    26221 ttgaagccat cttcactccg tacttcacca ccaaagccga aggcaccgga ttggggctgg
    26281 cggtcgtgca taatattgtt gaacaacacg gtggtacaat tcaggtcgca agccaggagg
    26341 gaaaaggctc aacgttcacc ctctggcttc cggtcaatat tacgcgtaag gacccacaag
    26401 gatgacgcac gataatatcg atattctggt ggtggatgat gacattagcc actgcactat
    26461 tttgcaggct ttactgcgcg gctggggcta taacgtcgcg ctggcgaaca gcgggcgaca
    26521 ggcgctggag caggtgcggg agcgggtttt tgatcttgtg ctttgcgatg tgcgaatggc
    26581 agagatggac ggcattgcca cgctaaaaga gatcaaagcg ttaaatccgg cgattccggt
    26641 actgattatg accgcatact ccagcgtcga gacggcggta gaggcactga aaaccggggc
    26701 gctggattat ctcatcaagc cgctggattt cgacaccctg caggcgacgc tggaaaaggc
    26761 gctcgcgcat acgcacagta ttgatgctga aacgcctgcg gtgactgcca gccagttcgg
    26821 tatggtcggt aaaagcccgg cgatgcaaca cctgctcagt gaaatcgccc tcgtcgcgcc
    26881 atcggaagcc acggtgctga tccacggcga ttccggcacc ggtaaagagc tggtcgccag
    26941 ggcgattcac gccagtagcg cacgtagcga aaaaccgctg gtaacgctca actgtgtggc
    27001 actcaacgaa tccttgctgg aatctgaatt gttcggtcac gaaaaagggg cgtttactgg
    27061 agccgataaa cgccgggaag ggcgctttgt tgaggcggac ggcggcacgc tgtttctcga
    27121 tgaaattggc gatatctcgc cgatgatgca ggtgcgtctg ctgcgtgcga ttcaggagcg
    27181 cgaagttcag cgtgtcggta gcaaccagac tatctcggtt gatgtccggc tgattgcggc
    27241 gacccatcgc gatcttgccg cagaggtgaa tgccgggcgt tttcgccagg atctctacta
    27301 tcgcctgaat gtggtggcga ttgaagtacc atcgctgcgg caacggcggg aggatatccc
    27361 tctgctggct agccattttc ttcagcgttt tgccgagcgt aatcgcaagg cggtaaaagg
    27421 ctttacgcca caggcgatgg atttgctgat ccactacgac tggccgggaa acattcgtga
    27481 actggaaaac gcggtggaaa ggtcggtggt gctgctgacc ggggaatata tttccgaacg
    27541 cgagctgccg ctggcgattg ccagtacgcc gatcccgctg gggcaaagtc aggatattca
    27601 gccgctggtg gaagtcgaaa aagaagtgat tctggcggca ctggagaaaa cgtgcggcaa
    27661 caaaaccgaa gccgcccgtc agttagggat cacgcgcaaa acgctattgg caaaactgtc
    27721 gcgttagttc tgctcgcgtt cgatagcgcg ccagccgata tctttccggc agaagcagtc
    27781 gtcccagtga atatcggtca ttaaggcata agcgcgtttc tgcgcttctg ccacggtatg
    27841 acccagcgcg gtgacgcaca gtacgcgccc gccgctggtc actacctgct cgttatccgc
    27901 cagtttcgtg cccgcgtgga acactttgcc gcctgccact tcttccagcg gcaggccgtg
    27961 gatcacgtca ccggtgcggt aatcacccgg atatccaccc gcagccatca ccacgccgag
    28021 agaagcgcgt tcatcccact ctgacgtttt ctcgtccagt ttgccttcac aggccgccag
    28081 gcagagctca accagatcgg acttcatgcg cagcataatc ggctgggttt ctggatcgcc
    28141 aaagcggcag ttaaattcga taaccttcgg attgccctgt ttgtcgatca tcaggcccgc
    28201 gtagagaaaa ccggtgtagg tgttgccttc cgccgccatg cctttcacgg ttggccagat
    28261 gatacgttcc atggtgcgct gatgaacttc atcggttact accggcgcgg gggagtaagc
    28321 gcccatcccg cccgtgtttg gtccggtatc tttatcgcct acgcgtttgt gatcctggct
    28381 ggtggccatc ggcagcacat gctcgccgtc taccatcacg ataaagctcg cttcttcgcc
    28441 atcgaggaac tcttcgataa cgatgcgatg acccgcgtcg ccaaaagcgt tgcccgccag
    28501 catatcgtga acagccgctt ccgcttcttc cagcgtcatc gccacgataa cgcctttccc
    28561 ggcagccaga ccgtccgctt taatgacgat tggcgcgcct ttctcacgca gatacgccag
    28621 cgcaggttct acctcggtga agttctggta ttccgccgta gggatcttat ggcgggccag
    28681 gaaatctttg gtaaacgctt ttgagccttc cagttgggcc gcacctgcgg ttgggccgaa
    28741 gattttcagc ccggcggcgc ggaaggtatc gaccacgcct ttcaccagcg gcgcttccgg
    28801 gccgacgatg gtcagatcaa tcttttcgtt ttgtgcgaaa tccaacagcg ccgggatatc
    28861 ggtcacgcca atagcaacgt tttgcagcgc gggttccagt gcagtgcctg cattacccgg
    28921 agcaacaaaa acagtctcaa ccagcggcga ctgggccgct ttccaggcca gcgcgtgctc
    28981 gcgcccgccg ttaccaataa ctaatacttt catctattgc tccattaatg gcggaagtgg
    29041 cgcatgtcgg tgaagagcat cgcaataccg tgctcgtcgg cggcggcaat cacttcgtca
    29101 tcacggatgg aaccgccagg ctggattacg caggtcacgc ccgcagcggc ggcggcatca
    29161 ataccgtcgc ggaacgggaa gaacgcgtca gaagccatcg aggaaccttt cacttccagg
    29221 ccttcatcgg cagctttaat accggcgatt ttcgcggagt acacgcggct catctggccc
    29281 gcgccaatac cgatggtcat attgtttttg gcatagacga tagcattgga tttcacgaac
    29341 ttcgccactt tccagcagaa cagcgcatcg cgcagttcct gttcggtcgg ctgacgttgg
    29401 gtgacgacgc gcagttcttc cgcaccgacc atccccaggt cacgatcctg aaccagcaga
    29461 ccgccgttca cgcgtttgaa atcaagaccc ggaacacgct cgccccactg accgcaggtc
    29521 agaacgcgta cgttctgctt ggcggcggtg attttcaggg cttcttcgct ggcggaaggc
    29581 gcaataatca cttcgacaaa ctgacgagaa atgatggcct gtgcggtttc cgcatctagc
    29641 tcgcggttaa aggcaatgat gccgccgaat gcggaggttg ggtcggtttt gtacgcgcga
    29701 tcgtaagcat caagaatgga attgccgata gctacaccgc aagggttggc gtgcttcaca
    29761 atcacacatg ccggctcggc gaactctttc acgcactcca gcgccgcatc ggtatcggcg
    29821 atgttgttat aagagagggc tttaccctga acctgggttg cggtagcaac ggaggcttct
    29881 ttcacattct cttctatata gaaggcagcc tgctggtggc tgttctcgcc gtaacgcata
    29941 tcctgcttct taatgaagtt caggttcagc gtgcgtggga agcgaccagc ggcttctttg
    30001 ctttcaccgt ggtacgccgg aaccatgctg ccgaagtagt tggcaatcat gctgtcgtag
    30061 gcggcagtgt gttcgaaggc tttgatggcg aggtcgaaac gggttgcaag cgtcagcgat
    30121 ccttcgttgt catccatctc tttaataatg gcgtcgtagt cgctgctctt caccacgatt
    30181 gcgacatctt tatggttctt agcggcggag cgcaccatgg tcgggccgcc gatatcgatg
    30241 ttctcaaccg catcttccag cgagcaacct tcacgggcca cggtctgggc gaacggatac
    30301 aggttaacaa ccaccatatc gataggctgg atctgatgtt cttccataat ggcatcgtcc
    30361 tggccgcgac ggcccagaat gccaccatgt actttcggat gcagggtctt cacgcgtcca
    30421 tccatcatct ccgggaaacc ggtgtaatcg gaaacttcgg ttaccggcag acctttttct
    30481 gctaacagac gggcagtgcc ccctgtagac agcagctcca caccgcgtgc ggaaagtgcc
    30541 tgggcgaatt cgacgatacc ggctttgtca gaaacactga gcagagcgcg gcggactgga
    30601 cgacgttgtt gcatggtaaa tcccctggat ttgactatta cagagagcgt tagctgaatt
    30661 tttcgcgaaa aactcagcta acgcccctaa cggggcatcc ttatttttcg cccacattgt
    30721 aacgaaaacg tttgcgcaac gctcgcgaat ttttctcttt caatggtgat cacaattttg
    30781 acagtggtta ccgtgggaaa aatacagaaa ttacattgat gattgtggat aactctgtgc
    30841 gtaaaaaggt ataaagcggg gttttgctgg ggaatgcagc agtcagtcat ttttctgcaa
    30901 tttttctatt gcggcctgcg gagaactccc tataatgcgc ctccatcgac acggcggatg
    30961 tgaatcactt cacacaaaca gccggttcgg ttgaaga
//
LOCUS       DAZGJW010000049        29890 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZGJW010000049 DAZGJW010000000
VERSION     DAZGJW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.049"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(29..1483)
                     /gene="xylB"
                     /locus_tag="ACHKIK_002188"
     CDS             complement(29..1483)
                     /gene="xylB"
                     /locus_tag="ACHKIK_002188"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO9083354.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="ACHKIK_002189"
     CDS             complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="ACHKIK_002189"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO9083355.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMAVALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3243..4235
                     /gene="xylF"
                     /locus_tag="ACHKIK_002190"
     CDS             3243..4235
                     /gene="xylF"
                     /locus_tag="ACHKIK_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9083356.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            4313..5854
                     /gene="xylG"
                     /locus_tag="ACHKIK_002191"
     CDS             4313..5854
                     /gene="xylG"
                     /locus_tag="ACHKIK_002191"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO9083357.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAEEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5832..7013
                     /gene="xylH"
                     /locus_tag="ACHKIK_002192"
     CDS             5832..7013
                     /gene="xylH"
                     /locus_tag="ACHKIK_002192"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO9083358.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7091..8269
                     /gene="xylR"
                     /locus_tag="ACHKIK_002193"
     CDS             7091..8269
                     /gene="xylR"
                     /locus_tag="ACHKIK_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO9083359.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8377..9201)
                     /gene="bax"
                     /locus_tag="ACHKIK_002194"
     CDS             complement(8377..9201)
                     /gene="bax"
                     /locus_tag="ACHKIK_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO9083360.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSNSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9521..11551
                     /gene="malS"
                     /locus_tag="ACHKIK_002195"
     CDS             9521..11551
                     /gene="malS"
                     /locus_tag="ACHKIK_002195"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO9083361.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTETFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTQPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASQVRVCPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDMTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11729..12982
                     /gene="avtA"
                     /locus_tag="ACHKIK_002196"
     CDS             11729..12982
                     /gene="avtA"
                     /locus_tag="ACHKIK_002196"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO9083362.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13134..13607)
                     /gene="ysaA"
                     /locus_tag="ACHKIK_002197"
     CDS             complement(13134..13607)
                     /gene="ysaA"
                     /locus_tag="ACHKIK_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO9083363.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHCWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13709..14557)
                     /gene="yiaJ"
                     /locus_tag="ACHKIK_002198"
     CDS             complement(13709..14557)
                     /gene="yiaJ"
                     /locus_tag="ACHKIK_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO9083364.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            14758..15756
                     /gene="yiaK"
                     /locus_tag="ACHKIK_002199"
     CDS             14758..15756
                     /gene="yiaK"
                     /locus_tag="ACHKIK_002199"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO9083365.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15768..16235
                     /gene="yiaL"
                     /locus_tag="ACHKIK_002200"
     CDS             15768..16235
                     /gene="yiaL"
                     /locus_tag="ACHKIK_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO9083366.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            16353..16826
                     /gene="yiaM"
                     /locus_tag="ACHKIK_002201"
     CDS             16353..16826
                     /gene="yiaM"
                     /locus_tag="ACHKIK_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFO9083367.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            16829..18106
                     /gene="yiaN"
                     /locus_tag="ACHKIK_002202"
     CDS             16829..18106
                     /gene="yiaN"
                     /locus_tag="ACHKIK_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO9083368.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            18119..19105
                     /gene="yiaO"
                     /locus_tag="ACHKIK_002203"
     CDS             18119..19105
                     /gene="yiaO"
                     /locus_tag="ACHKIK_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO9083369.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            19109..20605
                     /gene="lyxK"
                     /locus_tag="ACHKIK_002204"
     CDS             19109..20605
                     /gene="lyxK"
                     /locus_tag="ACHKIK_002204"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO9083370.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHTLPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            20602..21264
                     /gene="ulaD"
                     /locus_tag="ACHKIK_002205"
     CDS             20602..21264
                     /gene="ulaD"
                     /locus_tag="ACHKIK_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO9083371.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            21257..22117
                     /gene="sgbU"
                     /locus_tag="ACHKIK_002206"
     CDS             21257..22117
                     /gene="sgbU"
                     /locus_tag="ACHKIK_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO9083372.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            22111..22806
                     /gene="araD"
                     /locus_tag="ACHKIK_002207"
     CDS             22111..22806
                     /gene="araD"
                     /locus_tag="ACHKIK_002207"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO9083373.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(22838..22939)
                     /locus_tag="ACHKIK_002208"
                     /pseudo
     CDS             complement(22838..22939)
                     /locus_tag="ACHKIK_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            22943..23206
                     /locus_tag="ACHKIK_002209"
                     /pseudo
     CDS             22943..23206
                     /locus_tag="ACHKIK_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23153..23893)
                     /gene="yiaT"
                     /locus_tag="ACHKIK_002210"
     CDS             complement(23153..23893)
                     /gene="yiaT"
                     /locus_tag="ACHKIK_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO9083374.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITNSPMIDRNESFTFITGVSWRF"
     gene            24017..24991
                     /gene="yiaU"
                     /locus_tag="ACHKIK_002211"
     CDS             24017..24991
                     /gene="yiaU"
                     /locus_tag="ACHKIK_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9083375.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQQPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(24988..26124)
                     /gene="yiaV"
                     /locus_tag="ACHKIK_002212"
     CDS             complement(24988..26124)
                     /gene="yiaV"
                     /locus_tag="ACHKIK_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO9083376.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYKRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(26130..26453)
                     /gene="yiaW"
                     /locus_tag="ACHKIK_002213"
     CDS             complement(26130..26453)
                     /gene="yiaW"
                     /locus_tag="ACHKIK_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO9083377.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            26576..26779
                     /locus_tag="ACHKIK_002214"
     CDS             26576..26779
                     /locus_tag="ACHKIK_002214"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083378.1"
                     /translation="MAFQQVEVYHIEIMELLTKLMNNSSKTSTVQIKRIKPSIIYRLL
                     LIGLGTPMVIYGLVCPLTIETRE"
     gene            complement(26998..28536)
                     /gene="aldB"
                     /locus_tag="ACHKIK_002215"
     CDS             complement(26998..28536)
                     /gene="aldB"
                     /locus_tag="ACHKIK_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO9083379.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(28701..29852)
                     /gene="yiaY"
                     /locus_tag="ACHKIK_002216"
     CDS             complement(28701..29852)
                     /gene="yiaY"
                     /locus_tag="ACHKIK_002216"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO9083380.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         7406 a         7708 c         7470 g         7306 t
ORIGIN      
        1 ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt tgctgataga
       61 ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca tctggtagat
      121 gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct ggattcgccg
      181 caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc gtacggtaat
      241 cgagctgctg accgctgata tccgccagca tctgacgcca gtactcacta cgcgccccgc
      301 caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc atgcacgaca tccatgccat
      361 ctgccagcgc ataacccacg ccttccagca ctgctcgcgc cagttcattg gggccatgtt
      421 gatgagtcaa accaaagaaa acccccttcg cctggggatt attgtgtggt gtacgctcgc
      481 cggaaagata aggcagaaac caaactggct cggcactttc atcagcctgt tgagctgcag
      541 cgattaaagc tgggacattg cacaggccgg ttaatttcgc gacccaatcc agacacgatg
      601 ctgcactcag cattacagac attaaatgcc aacgctgcgg tagcgcatgg caaaagctgt
      661 gtacagcgct ttctggcttg cttaagaacc cttcgctgac agcaaaatag acccccgacg
      721 tccccagcga taacattgcc tgattagcat caaccattcc cacaccaact gcaccagctg
      781 cattgtcgcc accgcctgcg acaactggca ccgtcgccat accccacgct ttcgcaactt
      841 caggtaacaa agcaccagta atttcgctgc cttcgtataa tgcgggcatc tggtcacgag
      901 ataagtcgca agcctgcagc atgacgtcac tccagtcacg ctttgcgaca tccagccaca
      961 tggtgccagc tgcgtcagac atatcgctgg caaactcccc cgtcatacgc agacgcaagt
     1021 aatctttcgg taataatact ttgtcgattt gacggaatat ctccggctca tgccgctgaa
     1081 cccatagcaa tttaggcgca gtaaatccgg gcatcatcag gttgccggta atcactcgtg
     1141 attgcggaac tctcgcttcc agcaaagtgc actcttgcgc acagcgcccg tcgttccaca
     1201 aaatggcagg gcgcaatacc cgttgttgag catccagtaa ggttgctcca tgcatctggc
     1261 cggcaatacc caatgcttta acgtcctgca gagaatgctg atcgcccaga gctttcattg
     1321 cgcgatcagt tgcctgccac cactgttccg ggtcttgttc cgaccagagt ggatgcgggc
     1381 gcgaaacggt cagcttttcc gtttgcgaag caaccacctc accctgctcg ttgagcaaaa
     1441 taacttttac gcccgaggtg ccaagatcta tcccgatata catatcgatc gttccttaaa
     1501 aaaatgcccg gtatcgctac cgataaccgg gccaacggac tgcacagtta gccgttattt
     1561 gtcgaacaga taatgattta ccagattttc cagttgctcc tggcgaccac tctgatgcac
     1621 cggagacaaa ttatgttcct gagcatattt ggctaaatct gccagtgaca tttggccttt
     1681 caggatttgc tggcccaatt cgctattcca gccggaataa cgctgcgcga tgcgtttatc
     1741 cagctcgcca tcttcaatca tgcgcgctgc aattttcagc gccactgcca tcgtatccat
     1801 cgcgccgata tgaccgtaaa acagatcata tttatcagta ctttgacgac gtactttggc
     1861 atcgaagttc agaccaccgg tggtgaaacc acctgctttg agaatttcat acatcaccag
     1921 cgcattctct tccacactgt tcgggaactg gtcggtgtcc cagcccagtt gcgcatcgcc
     1981 acggttggcg tcgacagaac cgaacaggcc aagcgcaatg gcggtggcta tttcatgatg
     2041 gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg ttcagtttaa tctctttttc
     2101 cagaccaaac tgtttcagga agccatagac cgtcgcggca tcgtaatcat attgatgttt
     2161 agtcggttct tgcggtttcg gttcgataag caacgtgccc tggaagccga ttttatgttt
     2221 atgctcaacc accatctgca taaagcggcc cagttgttca cgctcctgac gcaagtcggt
     2281 atttaacagc gtttcgtaac cttcacgacc accccacagg acatagtttt caccgcccaa
     2341 tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc gcccagctga agacttcagg
     2401 atctgggttc gtcgccgcac ccgcgccata gcgagggttt gtaaagcagt tagcggttcc
     2461 ccacagcagc ttcacgccgc tctcttcttg cttggctgcc aggacatcaa ccatttgcgc
     2521 aaaattattg atgtactctt ttaacgacgc gccctcaggg gaaacatcca catcgtggaa
     2581 gcaataaaat ggcacatgta acttgtggaa aaactcaaat gcgacatctg ctttacgctt
     2641 cgccaacgcc agtgcctcac caggctgctg ccacggacga ttaaacgccc ccacaccaaa
     2701 catgtccgcc ccgttccagc agaaggtgtg ccagtagcag gcggcaaaac gcaagtgctc
     2761 ttccatacgc ttacccaaca ccagttcgtc gggattgtag tgacggaatg ctaacgggtt
     2821 tgaggatttt gagccttcat aacgaacgcg atcgagctgg tcaaaatagg cttgcatatt
     2881 gaactccata atcaggtaat gccgcgggtg atggatgatg tcgtaatatt gggcactccc
     2941 tttcagttgc tcaattatgt tatttcacac tgctattgag ataattcaca agtgtgcgct
     3001 cgctcgcaaa ataaaatggg atgatgaaac tgggtaattc ctcgaagata aaaatgcaat
     3061 aagtacaatt gcgcaacaaa agtaagatct cggtcataaa tcaagaaata aaccaaaaat
     3121 cgtaatcgaa agataaaaat ctgtaattgt tttcccctgt ttagttgcta aaaattggtt
     3181 acgtttatcg cggtgattgt tacttattaa agctgtcctc taactacaga aggccctaca
     3241 ccatgaaaat aaagaacatt ctactcaccc tttgcacctc acttctgctt accaacgttg
     3301 cggcacacgc caaagaagtc aaaataggta tggcgattga tgatctccgt cttgaacgct
     3361 ggcaaaaaga tcgagatatt tttgtgaaaa aggcagaatc tctcggcgcg aaagtatttg
     3421 tacagtctgc aaatggcaat gaagaaacac aaatgtcgca gattgaaaac atgataaacc
     3481 ggggtgttga tgttcttgtc attattccgt ataacggtca ggtattaagt aacgttgtaa
     3541 aagaagccaa acaagaaggt attaaagtat tagcttacga tcgtatgatt aacgatgcgg
     3601 atatcgattt ttatatttct ttcgataacg aaaaagtcgg tgaactgcag gcaaaagccc
     3661 tggtcgatat tgttccgcaa ggtaattact tcctgatggg cggctcgccg gtagataaca
     3721 acgccaagct gttccgcgcc ggacaaatga aagtgttaaa accttacgtt gattccggaa
     3781 aaattaaagt cgttggtgac caatgggttg atggctggtt accggaaaac gcattgaaaa
     3841 ttatggaaaa cgcgctaacc gccaataata acaaaattga tgctgtagtt gcctcaaacg
     3901 atgccaccgc aggtggggca attcaggcat taagcgcgca aggtttatca gggaaagtag
     3961 caatttccgg ccaggatgcg gatctcgcag gtattaaacg tattgctgcc ggtacgcaaa
     4021 ctatgacggt gtataaacct attacgttgt tggcaaatac tgccgcagaa attgccgttg
     4081 agttgggcaa tggtcaggaa ccaaaagcag ataccacact gaataatggc ctgaaagatg
     4141 tgccctcccg cctcctgaca ccgatcgatg tgaataaaaa caacatcaaa gacacggtag
     4201 ttaaagacgg attccacaaa gagagcgagc tgtaagcgtt acgccccggc gcggagcggg
     4261 ggcgtgattt ctctccatgc cgcgtgaatg aattggctta ggtggagtcg ttatgcctta
     4321 tctacttgaa atgaagaata ttaccaaaac cttcggcagt gtgaaggcga ttgataacgt
     4381 ctgcttgcgg ttgaatgctg gcgaaatcgt ctcactttgt ggggaaaatg ggtctggtaa
     4441 atcaacgctg atgaaagtgc tgtgtggtat ttatccccat ggctcctacg aaggcgaaat
     4501 tatttttgcg ggagaagaga ttcaggcgag tcacatccgc gataccgaac gcaaaggtat
     4561 cgccatcatt caccaggaat tggccctggt gaaagaattg accgtgctgg aaaatatctt
     4621 cctgggtaac gaaataaccc acaatggcat tatggattat gacctgatga cgctacgctg
     4681 tcagaagctg ctcgcacagg tcagtttatc catttcacct gatacccgcg ttggcgattt
     4741 agggcttggg caacaacaac tggttgaaat tgccaaggca cttaataaac aggtgcgctt
     4801 gttaattctc gatgaaccga cagcctcatt aactgagcag gaaacgtcgg ttttactgga
     4861 tattattcgc gatctacaac agcacggtat cgcctgtatt tatatttcgc acaaactcaa
     4921 cgaagtcaaa gcgatttccg atacgatttg cgttattcgc gacggacagc acattggtac
     4981 gcgtgatgct gccggaatga gtgaagacga tattatcacc atgatggtcg ggcgagagtt
     5041 aaccgcgctt taccctaatg aaccacatac caccggagat gaaatattac gtattgaaca
     5101 tctgacggca tggcatccgg tcaatcgtca tattaaacga gttaatgatg tctcgttttc
     5161 cctgaaacgt ggcgaaatac tgggtattgc cggactcgtt ggtgccggac gtaccgagac
     5221 cattcagtgc ctgtttggcg tgtggcccgg acaatgggaa ggaaaaattt atattgatgg
     5281 caaacaggta gatattcgta actgtcagca agccatcgcc caggggattg cgatggtacc
     5341 cgaagacaga aagcgcgacg gcatcgttcc ggtaatggcg gttggtaaaa atattaccct
     5401 cgccgcactc aataaattta ccggtggcat tagccagctt gatgacgcgg aagagcaaaa
     5461 atgtattctg gaatcaattc agcaactcaa agttaaaacg tcgtcccccg atcttgctat
     5521 tggacgtttg agcggcggca atcagcaaaa agcgatcctc gctcgctgtc tgttacttaa
     5581 cccgcgcatc ctcattcttg atgaacctac caggggtatc gatattggcg cgaaatacga
     5641 gatctacaaa ttaattaacc aactcgtcca gcagggtatt gccgttattg tcatctcttc
     5701 cgaattacct gaagtgctcg gccttagcga tcgggtactg gtgatgcatg aagggaaact
     5761 aaaagccaac ctgataaatc ataacctgac tcaggagcag gtgatggaag ccgcattgag
     5821 gagcgaacat catgtcgaaa agcaatccgt ctgaagtgaa attggccgta ccgacatccg
     5881 gtggcttttc cgggctgaaa tcactgaatt tgcaggtctt cgtgatgatt gcagctatca
     5941 tcgcaatcat gctgttcttt acctggacca ccgatggtgc ctacttaagc gcccgtaacg
     6001 tctccaacct gttacgccag accgcgatta ccggcatcct cgcggtagga atggtgttcg
     6061 tcataatttc tgctgaaatc gacctttccg tcggctcaat gatggggcta ttaggtggcg
     6121 tcgcggcgat ttgtgacgtc tggttaggct ggcctttgcc acttaccatc attgtgacgt
     6181 tggttctggg actgcttctc ggtgcctgga acggatggtg ggtcgcgtac cgcaaagtcc
     6241 cttcatttat tgtcaccctc gcgggcatgt tggcatttcg cggcatactc attggcatca
     6301 ccaacggcac gactgtttcc cccaccagcg ccgcgatgtc acaaattggg caaagctatc
     6361 tacctgccag cactggcttc atcattggcg cgcttggctt aatggctttt gttggttggc
     6421 aatggcgcgg aagaatgcgc cgtcaggctt tgggtttgca gtctccggcc tctaccgcag
     6481 tagtcggtcg ccaggcttta accgctatca tcgtattagg cgcaatctgg ctgttgaatg
     6541 attaccgtgg cgttcccact cctgttctgc tgctgacatt gctgttactc ggcggaatgt
     6601 ttatggcaac gcggacggca tttggacgac gcatttatgc catcggcggc aatctggaag
     6661 cagcacgtct ctccgggatt aacgttgaac gcaccaaact tgccgtgttc gcgattaacg
     6721 gattaatggt agccatcgcc ggattaatcc ttagttctcg acttggcgct ggttcacctt
     6781 ctgcgggaaa tatcgccgaa ctggacgcaa ttgcagcatg cgtgattggc ggcaccagcc
     6841 tggctggcgg tgtgggaagc gttgccggag cagtaatggg ggcatttatc atggcttcac
     6901 tggataacgg catgagtatg atggatgtac cgaccttctg gcagtatatc gttaaaggtg
     6961 cgattctgtt gctggcagta tggatggact ccgcaaccaa acgccgttct tgattttgat
     7021 aaaaattttc tcaaagccgg ttacgtatta ccggttttga gtttttgcat gattcagcag
     7081 gaaaagaacc atgtttacta aacgtcaccg catcacatta ctgttcaatg ccaataaagc
     7141 ctatgaccgg caggtagtag aaggcgtagg ggaatattta caggcatcac aatcagaatg
     7201 ggatattttc attgaagaag atttccgcgc ccgcattgat aaaatcaagg actggttagg
     7261 agatggcgtc attgccgact tcgacgacaa acagatcgag caagcgctgg ctgatgtcga
     7321 cgtccccatt gttggggttg gcggctcgta tcaccttgca gaaagttacc cacccgttca
     7381 ttacattgcc accgataact atgcgctggt tgaaagcgca tttttgcatt taaaagagaa
     7441 aggcgttaac cgctttgctt tttatggtct tccggaatca agcggcaaac gttgggccac
     7501 tgaacgcgaa tatgcatttc gtcagcttgt cgccgaagaa aagtatcgcg gagtggttta
     7561 tcaggggtta gaaaccgcgc cagagaactg gcaacatgcg caaaatcggc tggcagactg
     7621 gctacaaacg ctgccaccgc aaaccgggat tattgccgtt actgacgccc gggcgcggca
     7681 tattctgcaa gtatgtgaac atctacatat tcccgtaccg gaaaaattat gcgtgattgg
     7741 catcgataac gaagaactga cccgctatct gtcgcgtgtc gccctttctt cggtcgctca
     7801 gggcgcgcgg caaatgggct atcaggcggc aaaactgttg catcgattat tagataaaga
     7861 agaaatgccg ctacagcgaa ttttggtccc accagttcgc gtcattgaac ggcgctcaac
     7921 agattatcgc tcgctgaccg atcccgccgt tattcaggcc atgcattaca ttcgtaatca
     7981 cgcctgtaaa gggattaaag tggatcaggt actggatgcg gtcgggatct cgcgctccaa
     8041 tcttgagaag cgttttaaag aagaggtggg tgaaaccatc catgcaatga ttcatgccga
     8101 gaagctggag aaagcgcgca gtctgctgat ttcaaccacc ttgtcgatca atgagatatc
     8161 gcaaatgtgc ggttatccat cgctgcaata tttctactct gtttttaaaa aagcatatga
     8221 cacaacgcca aaagagtatc gcgatgtaaa tagcgaggtc atgttgtagc gatgatgaga
     8281 attgtcggcg tcacatcagg gaactcagca cagccacctg atgcgagcaa aagccttatc
     8341 cgaccgacaa atcagataag gctcaaggaa atgcaattac atatgcgccg cgattaaccg
     8401 ttggttatcc tggtacattg cgaacaggta gttgttataa ctcttccctt tggtcgagta
     8461 gcccttcagc ttgtgaatca tcgctgtggc agtcacttcc tgatccgctt tacgcagctg
     8521 cgcacgcgat ttacggaatg aagagtaagc cggatgcgta ttgaggttag tgacataggc
     8581 gctcaccgat tctttgacag aactaaactg tgagtaccct ttcactttac ccggcgcatt
     8641 ggtacaacgt cctttcatgc atttcatgcc gaacagattg ttgttgttgc gcgccagctt
     8701 tgacgttccc caaccgcttt ctgctgcagc catcgtcgcc accatactgg tggggataat
     8761 gtctacgcgt tcaagcaagg tattccacgg gatttttcgc gtattaccag accacttcac
     8821 cttgtagcgt ttggcgatgt ctttcagacg cgcacgctca gcaggtgacc attggccctg
     8881 atactgtttt gaaatcagcc agttacgttc cgcagtaatg gctgcatttt ggctggtaat
     8941 gtaaggcatt acggtccgga gaaacgcctt tttccttggt gttccggaag ggtattttcg
     9001 caaatcagga agtgaattac tctttgcact attgcgagaa tactcttgtt tactgcttac
     9061 ctgtttatta ctagctttag ttaagtggga cttttgactc gctgttgttg tgtgcgtctt
     9121 cgctaacacc tcactcgaaa acaccagagt gagtaacata agaatcatcg ccccatatcg
     9181 tcgtatggga gtcaaaatca tcaggtctcc tggtcggatt taatcattcc aacaccttat
     9241 atttttcaca aatttgagag ttgaatctca aatcatatca aaaatagctg tcaagagcac
     9301 cccaaggaat agtccaaatc tgaaactatg tcacgagtta acgattcaga ttggcgctaa
     9361 atcgcagaaa atgtggggtt tatcgcaaaa ttcagccgtt ttttgcgcga gatcgctcac
     9421 ccttgcttct catcctgtgg acttaccgct cagggatgag ttttgtttgg cttatcgctg
     9481 gcaaactgtc tgaaatcgca gcaataagga ctcatccgcc atgaaactcg ccgcctgttt
     9541 tctgacactc cttcctggct tcgccgttgc cgccagctgg acttctccgg ggttccctgc
     9601 ctttagtgaa cagggaacgg aaacatttgt cagccacgcg cagttgccca aaggtacgcg
     9661 tccactaacg ctaaattttg accaacagtg ctggcagcct gcggatgcga taaaactcaa
     9721 tcagatgctt tccctgcaac cttgtagcaa cacgcagcct caatggcgat tgttcaggga
     9781 cggcgaatat acgctgcaaa tagacacccg ctccggcacg ccaacattga tgatttccat
     9841 ccagaacgcc gccgaaccgg tagcaagcca ggtccgtgta tgcccgaaat gggatggatt
     9901 accgctcacg ctggatgtca gcgccacttt cccggaagga gccgccgtac gggattatta
     9961 cagccagcaa attgcgatag tgaagaacgg tcaaataacg ttacaacccg ctgccaccag
    10021 caacggttta ctcctgctgg aacgggcaga aactgacgcc tctgcccctt tcgactggca
    10081 taacgccacg gtttactttg tgctgacaga tcgtttcgaa aacggcgatc ccagtaatga
    10141 ccagagttac ggacgtcata aagacggtat ggcggaaatt ggcacttttc acggcggcga
    10201 tttacgcggc ctgaccaaca aactggatta cctccagcag ttgggcgtta atgctttatg
    10261 gataagcgcc ccatttgagc aaattcacgg ctgggtcggc ggcggtacaa aaggcgattt
    10321 cccgcattat gcctaccacg gttattacac acaggactgg acgaatcttg atgccaatat
    10381 gggcaacgaa gccgatctac ggacgctggt tgatagcgca catcagcgcg gtattcgtat
    10441 tctctttgat gtcgtgatga accacaccgg ctatgccacg ctggcggata tgcaggagta
    10501 tcagtttggc gcgttatatc tttctggtga cgaggtgaaa aaaacgctgg gtgaacgctg
    10561 gagcgactgg aaacctgccg ccgggcaaac ctggcatagc tttaacgatt acattaattt
    10621 cagcgacaaa acaggctggg ataaatggtg gggaaaaaac tggatccgta ccgatatcgg
    10681 cgattacgac aatcctggat tcgacgatct caccatgtcg ctagcctttt tgccggatat
    10741 caaaaccgaa tcaactaccg cttctggtct gccggtgttc tataaaaaca aaacggatac
    10801 ccacgctaaa gccatcgacg gctttacccc tcgcgattac ttaacccact ggttaagtca
    10861 gtgggtccgc gactatggga ttgatggttt tcgggtcgat accgccaaac atgttgagtt
    10921 gcccgcctgg cagcaactga aaaccgaagc cagcgccgcg cttcgcgaat ggaaaaaagc
    10981 taaccccgac aaagcattag atgacaaacc tttctggatg accggtgaag cctggggcca
    11041 cggcgtgatg caaagtgact actatcgcca cggcttcgat gcgatgatca atttcgatta
    11101 tcaggagcag gcggcgaaag ctgtcgattg tattgcgcag atggatatga cctggcagca
    11161 aatggcggag aaattgcagg gtttcaacgt gttgagctac ctctcgtcgc atgatacccg
    11221 tctgttccgt gaagggggcg acaaagcagc agagttatta ctattagcgc caggcgcggt
    11281 acaaatcttt tatggcgatg aatcctcgcg tccgttcggt cctacaggtt ctgatccgct
    11341 gcaaggtaca cgttcggata tgaactggca ggatgttagc ggtaaatctg ccgccaacgt
    11401 cgcgcactgg cagaaaatca gccagttccg cgcccgccat cccgcaattg gcgcgggcaa
    11461 acaaacgaca ctttcgctga agcagggcta cggctttgtt cgtgagcatg gcgacgataa
    11521 agtgctggtc atctgggctg ggcaacagtg acttttccgg cttcccgttc gtcagtacct
    11581 cgggaagccg ccaaccagga taaaatgtca gccctaatca gcgttgcagg ataaagcacc
    11641 gctcactctt caacagaccg atttgcaccc cagcaaatgt agcgttattg ttaccttcct
    11701 tgctacagag ttcgacagat atcccgctat gacattctcc ctttttggtg acaaatttac
    11761 ccgccactcc ggcattacgc tgctgatgga agatctgaac gacggtttac gcacccctgg
    11821 cgcgattatg ctcggcggcg gtaatccggc gcagatcccg gaaatgcagg actacttcca
    11881 gacgctactg accgacatgc tggaaagtgg caaagcgact gatgcactgt gcaattacga
    11941 cggtccacag gggaaaacgg agctactcac actgcttgcc ggaatgctgc gcgagaagtt
    12001 gggttgggat atcgaaccac agaatattgc actaacaaac ggcagccaga gcgcgttttt
    12061 ctacttattt aatctgtttg ccggacgccg tgccgatggt cgggtcaaaa aagtgctgtt
    12121 cccgctggca ccggaataca ttggctacgc tgacgccgga ctggaagaag atctgtttgt
    12181 ctctgcgcgt ccgaatattg aactgctgcc ggaaggccag tttaaatacc acgtcgattt
    12241 tgagcatctg catattggcg aagaaaccgg gatgatttgc gtctcccggc cgacgaatcc
    12301 aacaggcaat gtgattactg acgaagagtt gctgaagctt gacgcgctgg cgaatcaaca
    12361 cggcattccg ctggtgattg ataacgctta tggcgtcccg ttcccgggta tcatcttcag
    12421 tgaagcgcgc ccgctatgga atccgaatat cgtgctgtgc atgagtcttt ccaagctggg
    12481 gctgcctggc tcccgctgcg gcattatcat cgccaatgaa aaaatcatca ccgccatcac
    12541 caatatgaac ggcattatca gcctggcacc tggcggtatt ggtccggcga tgatgtgtga
    12601 aatgattaag cgtaacgatc tgctgcgcct gtctgaaaca gtcatcaaac cgttttacta
    12661 ccagcgtgtt caggaaacta tcgccatcat tcgccgctat ttaccggaaa atcgctgcct
    12721 gattcataaa ccggaaggag ccattttcct ctggctatgg tttaaggatt tgcccattac
    12781 gaccgagcag ctctatcagc gcctgaaagc acgcggcgtg ctgatggtgc cggggcacaa
    12841 cttcttccca gggctggata aaccgtggcc gcatacgcat caatgtatgc gcatgaacta
    12901 cgtaccagag ccggagaaaa ttgaggcggg ggtgaagatt ctggcggaag agatagaaag
    12961 agcctgggct gaaagtcact aaaaattgcc tgatgcgcta cgcttatcag gcctacatga
    13021 tttctgcaat atattgaatt tggaagaatt tgtaggccgg ataaggcgtt tacgccgcat
    13081 ctggcataaa caaagcgcac tattccggcg ttgagaaacg ccggaaaaac gtttcaaaca
    13141 ggctgctgcc gtagccgatg ccgctgcact ttctcgacat ccatgcactg caacgccttt
    13201 gtcgggcagg cttcaacaca ggccggtccc gtctcccgat gccagcacag atcgcactta
    13261 atcgccctcg cctttttgcg cgaagaaacg acctccatcg caccaaacgg gcaagccagc
    13321 atacagcttt tacagccaat gcaacgtgtt tgttcaacga aaatatgccc atgttcgcgg
    13381 cttatcgcgt caacagggca gacattcgcg cacggtgcat cttcacactg atgacaggct
    13441 actgccgtgg tccagcagtg gtctttaatg acacgaatac gggaaataaa ctcgtctggt
    13501 gacaacgcag cgcaatcctg attctcctga tgcgacactg cgcaagccac ttcacaggta
    13561 cggcaaccga tacatttcgt cgcatccgca ataataaacc ggttcatgat cactcctttg
    13621 cactaaaaca gcagagtgcc agagagcgcg tccctactgc tttgatccaa cgaaggaaat
    13681 gttaaagttt tggcacttgt ccaaagcgtt atgtgatcgc gcccaggtca tccctgacag
    13741 taaatcccag ttcattagaa atagcctgcg cggtttcacg cagtggtttc aggagatttt
    13801 tctctcccac ctgtttcaga cgtgatgtcg aaagcgaaat cgacacggcg tacggcaccc
    13861 gcccatgaat atcaaacacc ggaacagcaa tacaggagac gccgagttcg ttttcttctc
    13921 tgtccatcgc cgctccgctt tcacgaatgt gcgccagttc gtcgaacatc gcgggcagct
    13981 cggtaatggt attgcgggtt aacggctgga tctcatgctg atggttttcc cagtatgact
    14041 tcacgtagtc cgggtgacca aacgccatgt agatcttgcc cattgcggaa cagtagagcg
    14101 gcatatgctg gccaatatag gcacgggttc gcagcatccc ggttgtgggt tccagcttat
    14161 aaatcaaaat agcgtgatcg tcttcgcggc tggagaagtt aatggtttca ccagtggcga
    14221 tgttcagtgc ctcaagatgc ggagcggcga tatgaatgat attcagcgaa gacagcgcct
    14281 tctgcccgac ggcaataaat ttggtagtca ggcggtaact ccctgcggcg ggcgcggtgg
    14341 tcacatagcc gcaggactgt aatccctgca ataagcgatg gacggtactc ttatttaaac
    14401 cagccagctc cgaaagatgc gccaacggac aaccgtttgg atagttgctc aaaatctcaa
    14461 tcagcatcaa cccgcgaaac agactctggc ttccggctgg acgctctttt tcctgcgcca
    14521 tctcgttctc tttttttccc atcacttctt tccccatttt gtcgcgtcct gatggtagcg
    14581 caaagtgtgc cgtagttcac gatctcgaca gataatttat aaccacttga tttttatgtc
    14641 ttttgaaatt catcaatcag attgctttgt taaaaagtga tcgatatatt tgaaatcaag
    14701 tttcgcatat tgaaatttta agccaaaaaa gcgatcaaaa aaacaaggaa gcctgggatg
    14761 aaagtgacat ttgagcagtt aaaagcagcc tttaatcggg tcttaatttc acgcggcgtt
    14821 gacagcgaaa cggctgacgc ctgtgcagag atgttcgccc gcaccaccga atccggcgtt
    14881 tattctcacg gcgttaatcg tttccctcgt ttcattcaac aactggaaaa cggcgatatc
    14941 attcctgatg cccaacccaa acgtataacc agcctcggcg caattgaaca gtgggatgcc
    15001 cagcgttcga tcggcaacct gacggcgaaa aagatgatgg atcgcgccat tgaactggct
    15061 gccgatcacg gtattggtct ggtggcactt cgtaatgcca accactggat gcgcggcggc
    15121 agctacggct ggcaggcggc ggaaaaaggc tatattggca tttgctggac caactccatc
    15181 gccgtaatgc cgccgtgggg cgcaaaagag tgtcgcattg gcactaaccc gctgatcgtc
    15241 gccattcctt ccacgccgat caccatggtc gatatgtcga tgtcgatgtt ctcttacggc
    15301 atgttagaag ttaaccgcct ggcgggccgt cagctcccgg tcgatggtgg ctttgatgat
    15361 gagggcaatt tgaccaaaga acctggcgtt atcgagaaga atcgccgcat tttgccgatg
    15421 ggctactgga aaggttctgg catgtcgatt gtgctggata tgatcgctac tctcctttcc
    15481 gacggcgcat cggttgccga agtcacccag gacaacagcg acgaatacgg catttcacaa
    15541 atttttattg ccattgaagt ggacaagctt atcgacggtc ccacccgcga tgccaagctg
    15601 caacgtatca tggattacgt tactaccgct gaacgcgctg acgaaaacca ggccatccgc
    15661 ttacccggcc acgaatttac taccctgctg gccgaaaacc gccgtaacgg tattaccgtt
    15721 gatgacagcg tgtgggccaa aatccaggcg ttataaggag ataagtcatg atttttggac
    15781 atatcgcgca gcccaatccg tgccgtttgc ccgccgccat tgaaaaggcg ctcgattttc
    15841 tgcgcgctac cgatttcaac gccctggagc cgggcgttgt cgaaatcgac ggcaaaaata
    15901 tttatgcgca aattatcgat ttaaccacac gcgaagcggt ggaaaaccgt ccggaagtgc
    15961 atcgtcggta tatcgatatt cagtttctgg cgtggggtga agagaaaatc ggcattgcta
    16021 ttgatacggg aaataataaa gtcagcgaat cactattaga gcagcgcgat attatttttt
    16081 atcatgacag tgaacatgaa tcatttatag aaatgatacc gggcagctac gcaattttct
    16141 ttccgcagga tgttcaccga ccaggttgta ttttgcaaac agcctctgag atacgaaaaa
    16201 ttgtggttaa agtcgcatta acggcgctga attaatcgaa taatcgtcta cgccagaacg
    16261 actgggtaat gttattgctc taactatatt aataatttag gttgctgcac atgcaacttg
    16321 aattatgaca gacatagcct caaggaatag ctatgaaaaa aatacttgaa gcaatactgg
    16381 cgattaatct cgccgtactt tcctgtattg tgtttataaa cattatttta agatatggtt
    16441 ttcagacaag cattttatct gtagatgaat tgtcacgtta tttatttgtc tggttaacat
    16501 ttattggcgc gattgtggct tttatggata acgcccacgt tcaggtcacc tttctggtgg
    16561 agaaactctc ccccgcatgg caacgacgag tcgctctggt tacccattcc ttaatcttat
    16621 ttatttgtgg cgcactggcc tggggcgcaa cgctaaaaac aattcaggac tggagcgatt
    16681 attcaccgat cctcggttta cccatcggcc tgatgtatgc cgcttgtctg cccaccagcc
    16741 tcgttatcgc attctttgaa ctgcgtcatt tatatcaact catcacgcgt agcaattctc
    16801 taacgtcgcc accgcaagga gcttaatcat ggctgtgctg atttttctgg gctgtctgtt
    16861 gggtggtatt gctatcggtt tgcctattgc ctgggcactg ttgttgtgcg gggcggcgtt
    16921 aatgttctgg ctggacatgt ttgatgtcca gatcatggca caaacgctgg tgaacggagc
    16981 cgatagcttc tccctgctgg cgattccgtt ctttgtgctg gcgggtgaaa tcatgaatgc
    17041 gggcggcttg tcaaaacgca ttgttgacct gccgatgaaa ctggtggggc ataaaccagg
    17101 cgggctgggc tacgtcggcg tgctggcagc tatgattatg gcgagccttt ctggttctgc
    17161 cgttgcggat accgccgccg ttgccgctct actggtgccg atgatgcgca gcgccaacta
    17221 cccggtcaac cgggcggcgg ggctgattgc ctctggcggc attatcgcgc caattattcc
    17281 cccttccatt ccgtttatta tcttcggcgt ttccagcgga ttatccatca gtaagctgtt
    17341 tatggcgggc attgcccctg gcatgatgat gggcgcaacg ctgatgctta cctggtggtg
    17401 gcaggccagt cgccttaatt taccccgcca gcaaaaagca acgatgcagg aaatctggca
    17461 ctcttttgtc tccggtatct gggcgctgtt tcttccagtc atcattattg gcgggtttcg
    17521 ctccggtctg tttaccccta ccgaagccgg ggccgttgct gctttctatg cgctgtttgt
    17581 cgccacagtt atttaccgtg aaatgacctt cgccacactt tggcatgtac tcattggcgc
    17641 ggcgaaaacc acctcagtgg ttatgtttct ggtggcctcg gcacaagttt ccgcctggct
    17701 gattaccatt gctgaactgc cgatgatggt ttctgattta ctgcaaccgt tggtcgattc
    17761 accgcgtctg ctgtttatcg tcattatggt ggctatcctg attgtcggca tggtcatgga
    17821 tttaacgccg acggtattaa ttcttacccc ggtattaatg cctttagtta aagaggcagg
    17881 aatcgatccg atttatttcg gtgtcatgtt tatcattaac tgctcaatcg gcttaattac
    17941 accgcctatc ggtaatgtac ttaacgttat ttcaggggtg gcaaaactca aattcgatga
    18001 tgcagtcaga ggcgttttcc cttacgtcct ggttttatat tcattattag tcgtatttgt
    18061 ttttattccc gatctcatca tcctcccttt gaaatggatt aattaaaagg aaaatattat
    18121 gaaattacgc tctgtaacct acgcattatt cattgctggc ctggctgcat tcagcacatc
    18181 ttctctggcg gcgcaatctt tacgtttcgg ttatgaaacc tcacaaaccg actcgcaaca
    18241 tattgcggcg aaaaaattca atgatttatt gcaggagaga accaaaggcg agctgaaatt
    18301 aaaattgttt ccggatagca ccctcggtaa cgcgcaggcg atgatcagcg gcgtacgtgg
    18361 cggcaccatc gatatggaaa tgtccggctc gaataacttt gccggattat caccagtgat
    18421 gaacttgctt gatgtccctt tcctgttccg cgataccgct cacgcgcata aaacgctcga
    18481 cggcaaagtc ggtgatgatc tgaaagcctc acttgaaggt aaaggactaa aagtactggc
    18541 ctactgggaa aacggctggc gcgatgtcac caactcgcgc gcaccggtta aaacccccgc
    18601 cgacctgaaa gggctgaaaa ttcgcaccaa caatagcccg atgaatatcg ccgcattcaa
    18661 agtctttggc gctaacccga tcccgatgcc gtttgccgaa gtctataccg ggctggaaac
    18721 ccgcactatc gacgctcagg aacacccgat caacgtcgtc tggtcagcaa aatttttcga
    18781 agtgcagaag tacctttctc tgacgcacca cgcctattcc ccgcttctgg tggtgatcaa
    18841 caaagcgaag tttgatggct taagcccgga gttccagcaa gcgctaattt catctgcgca
    18901 ggaagcgggt aactaccagc gcaaactggt tgccgaagat cagcaaaaaa tcatcgacgg
    18961 catgaaagaa gcgggcgtgg aagtcatcac cgatctcgac cgcaaagcct ttagcgacgc
    19021 actgggtact caggtccgcg acatgtttgt aaaagatgtt ccgcagggtg ctgatctact
    19081 gaaagccgtg gatgaggtgc aataaaccat gacgcaatac tggctggggt tagattgtgg
    19141 cggtagctgg ctgaaagccg ggctgtatga ccgcgaaggc cgggaggcag gcgtgcagcg
    19201 cctgccgctg tgcgcattaa gcccgcagcc aggttgggca gagcgcgata tggcagaact
    19261 gtggcaatgc tgcatggctg tcattcgcga actgcttacc cattccggcg ttagcgggga
    19321 acaaattgtc ggtatcggta tctccgcaca aggaaaaggc ttgtttttgc tggataaaaa
    19381 caacaagccg ctcggtaacg ccattttatc ctcggatcgc cgggcgatgg aaatcgttcg
    19441 tcgctggcag gaagatggca tcccggaaaa actctacccg ctgacccgac aaaccttgtg
    19501 gaccgggcat ccggtatcgc tgttacgttg gcttaaagag cacgaaccgg aacgctacgc
    19561 gcaaattggc tgcgtgatga tgacgcacga ctacctgcgc tggtgtttaa ccggcgtcaa
    19621 aggctgtgaa gagagcaata tttccgagtc caatctctac aacatgagtc ttggggaata
    19681 tgacccgtgc ctcaccgact ggctggggat tgctgaaatc aatcacaccc tgccgcctgt
    19741 tgtcggatct gccgaaatct gcggggagat caccgctcag acagccgtac tgaccggtct
    19801 gaaagcgggt acgcccgttg tcggcggcct gtttgatgtg gtttccaccg cactctgcgc
    19861 cgggatcgaa gacgaattta cccttaatgc ggtgatgggc acctgggcgg tgaccagcgg
    19921 cataacccgc ggtttacgtg acggtgaagc gcatccgtat gtctatggtc gctacgttaa
    19981 cgatggtgaa tttatcgttc acgaagccag ccccacctct tccggcaacc tcgaatggtt
    20041 taccgcacag tggggagaaa tctcgtttga tgagatcaac caggccgttg ccagcttgcc
    20101 gaaggccggt ggcgatctct ttttcctgcc gttcctgtac ggcagcaacg ccggactcga
    20161 gatgaccagc ggtttctacg ggatgcaggc cattcacacc cgcgcacacc tgttgcaggc
    20221 catttatgaa ggggtggtgt tcagccatat gacccatctc aaccggatgc gcgaacgttt
    20281 tactgatgtg cacaccctgc gcgtcactgg cggcccggcg cattccgatg tctggatgca
    20341 aatgctggcg gacgtcagcg gtctgcgtat tgagctgccg caggtggaag aaaccggctg
    20401 ctttggtgca gcacttgccg cccgcgtcgg caccggggtt tatcgcgatt tcagcgaagc
    20461 ccagcgtgat ttacagcacc cggtgcgcac cctgctgccg gatatgactg tccatcagct
    20521 ttaccaacaa aaataccaac gctatcagca tctcattgcc gcacttcagg gctttcacgc
    20581 ccgcatcaag gagcacatat tatgagccga ccacttctgc aactggcact cgaccactca
    20641 tcacttgaag ccgcacagcg cgacgtgacg cagttaaaag acagcgtcga tattgtcgaa
    20701 gcgggcacca ttctctgttt aaacgaaggg cttggcgcgg tgaaagcctt gcgcgaacag
    20761 tgccctgaca aaatcatcgt tgccgactgg aaggtcgccg acgctggtga aacgctcgcg
    20821 caacaggcat ttggcgcagg cgctaactgg atgaccatca tctgcgccgc accgctcgcg
    20881 acggtggaaa aaggccacgc gatggcgcaa cgctgcggtg gtgaaattca gatcgagctg
    20941 ttcggtaact ggacgctgga cgacgcccgc gactggcatc gtattggcgt gcggcaggcg
    21001 atttatcatc gcggtcgtga tgcacaggcc agcgggcaac agtggggcga agccgatctg
    21061 gcacgcatga aggcgctttc cgatatcggc cttgagcttt ccattaccgg cgggattacc
    21121 cctgctgacc tgccgctgtt taaagatatt cgcgtgaaag cgtttattgc cgggcgagca
    21181 ctggcaggct cagcaaatcc ggcgcaggtg gctggcgatt tccatgcgca aatcgacgct
    21241 atctggggag gcaagcgtgc gtaatcatcc gttagggatt tatgaaaagg cgctggcgaa
    21301 agatctctcc tggccggagc ggctggtgct ggcaaaaagc tgcggttttg attttgtcga
    21361 aatgtcggtg gatgaaaccg acgaacggct ttcacgtctt gactggagca ccgcacaaag
    21421 gacttcgctg gttgccgcga tgatcgaaac gggtgttggg atcccgtcga tgtgcctgtc
    21481 cgcacatcgt cgctttcctt ttggtagccg tgacgacgcg gtacgccagc gagcacggga
    21541 gatcatgagc aaagcgattc gtctggcacg cgatctcggc atacgcacca tccagctggc
    21601 gggctacgac gtctattacg aagaccacga tgaaggcacc cggcaacgtt ttgctgaagg
    21661 gctggcgtgg gcggtcgaac aggcggcggc atcacaagtc atgctagcgg tggagattat
    21721 ggacaccgcg tttatgaact ccatcagtaa atggaaaaag tgggacgaga tgctcgcctc
    21781 gccgtggttc accgtctacc cggacgtcgg caacctcagc gcctggggca atgatgttcc
    21841 cgccgaactg aaactgggca ttgaccgtat cgccgcgatc cacctgaaag acacccagcc
    21901 agttaccgag caaagtcccg gacagttccg cgacgtgccg tttggcgaag gctgcgtcga
    21961 tttcgttggc atcttcaaaa cgctgcataa actgaactat cgcggttctt tcctgattga
    22021 gatgtggacc gaaaaagcca aagagccggt gctggagatt attcaggcgc ggcgttggat
    22081 tgaagcgcgt atgcaggagg ctggatttat atgttagagc aactgaaagc cgacgtgctg
    22141 gcggcgaatc tggcgcttcc cgctcaccat ctggtgacgt tcacctgggg caatgtcagc
    22201 gcggtagacg aaacgcggca atggatggta atcaaacctt ccggcgtcga gtatgacgtg
    22261 atgaccgccg acgatatggt ggtggttgag atagccagcg gcaaggtggt ggaaggtagc
    22321 aaaaagccct cgtccgacac gccaacgcat ctggcgcttt accgtcgcta cgccgaaatc
    22381 ggtggcattg tgcataccca ctcacgccac gccaccatct ggtcgcaggc cgggctggat
    22441 ctccccgcct ggggcaccac gcacgctgat tatttctatg gtgccatccc ctgcacgcga
    22501 cagatgaccg cagaggagat taacggcgaa tatgaatatc agaccggcga agtgatcatt
    22561 gaaaccttcg aagaacgtgg caggagtccg gcacaaatcc cggcggtgct ggtgcattct
    22621 cacggcccgt tcgcatgggg taaaaacgcc gccgatgccg tgcataacgc cgtagtactc
    22681 gaagaatgcg cctatatggg cctattctcg cgccagcttg cgccgcagct ccctgcgatg
    22741 caaaacgaac tgctggataa gcactacctg cgtaagcatg gggccaatgc ctattacggg
    22801 cagtaatccc tcacgccggg gcttcatcgc cccggcacta cgaattgata tgttccttgc
    22861 tgtaacgccg cttccacgct gctggcgtta aaccggtgtg cttgcgaaaa atctgccgga
    22921 aatagccaac gtcattaaac cgtattgcat ggagcaggcc gacaacgtcg agtcactgca
    22981 taacctgttg ctgcccgccg atgcgccatt tacgacattt gaaggcaagg gattgttcag
    23041 ccataagatc ctgattcaga cgccaggttc ccggtatgaa cagagcaatc cagagcaaca
    23101 acctttgtgg cattacgacg gagcaccagc cgcaccgaca tccaccggtg aattaaaaac
    23161 gccagctcac gccggtgata aaggtaaaac tctcattacg atcaatcatc gggctgttgg
    23221 taatctcttc cggcagctcg ctgtatcctg cgctcgccat caatacgacg tgctctccta
    23281 tcgggtattt tgccgtcagg ctgacatagg gcacccaggc atcctgcgcg gaataacttg
    23341 ccagaccgct acgacgggac tcactctctg aaatgccata gtaatagtca ctgaaattct
    23401 cgtcataata gagaacgccc agcgcaggtg tcagcgacag gcgacctatc tgcattttgt
    23461 ggaataccga tagctccccc acccagccgt tgctgttatc cagaacgtcc gcagccgcag
    23521 aggctttcac ggttccccaa cgctcgtggt gataccaggc aacccccgcc atagccgtac
    23581 tatcacgctt atcaagctgt tgcatggcat aatcgtcatt atcggcaggg tcaaattcca
    23641 gcggcatcca ggatgcggtc aggctaagtt catttttttc actttgcgac agaataaaac
    23701 ctaatgtggt ctgacgaaca taaaaagaat caccttcata actaatcagc ggaattgcct
    23761 ttgtattttc attataaccg cgataaggcg attcattata agctgcacca gcaccaatgg
    23821 ataattcaga agcagttgcg cttgccataa aaggcaacgc aaataacgcc acaatattgc
    23881 gattaattaa catgtccggt attccatttt aaaataagat aaaaaagagt cggcataata
    23941 atgcttacaa tttctccggt gcaagattca tatttatata gtaatatata aaattatata
    24001 taattgggct gttgcgatga caaaattaca acttaagtac cgggagttaa aaattatctc
    24061 ggtaatcgct gccagtgaaa atatcagcca tgccgcgact gtacttggca tcgcacaggc
    24121 caacgtcagc aaatatcttg ctgattttga atcaaaagtg ggtttaaaag tttttgaccg
    24181 aaccacccgg cagctgatgc tcacaccttt tggcaccgcg ctgttaccgt acatcaacga
    24241 catgctggac agaaacgagc aacttaataa ttttattgca gattataagc atgagaaacg
    24301 tggtcgggtc accatctacg ccccaaccgg cataattacc tatttatcca aacatgtaat
    24361 cgataaaatt aaagatatcg gtgatatcac cttatcgcta aaaacctgca atcttgagcg
    24421 taatgccttt tatgaaggcg tcgaatttcc tgatgattgt gatgttttaa tcagctacgc
    24481 tccaccaaaa gatgaatcgc tggttgccag ttttattacc caatatgccg taaccgctta
    24541 tgccagccag cgctatcttg agaaacatcc cattagccgt cctgacgaac tcgaacatca
    24601 ctcctgtatt ttgatcgact cgatgatgat cgacgacgcg aatatctggc gcttcaacgt
    24661 cgccgggagt aaagaggtgc gggattatcg cgtgaaggga aattacgttt gcgacaacac
    24721 gcaatctgcg ctggagctgg cacgaaatca cctggggatt gtgtttgcgc cagacaaaag
    24781 tgtgcagagc gacctgcaag acggcacgct ggtgccctgc tttcagcaac cctatgaatg
    24841 gtggctggat ctggtggcta tcttccgtaa acgggaatac cagccctggc gggtccagta
    24901 tgtgctggat gaaatgctgc gtgaaattcg ccaccagctt gctcagtcgc agcaactgcg
    24961 gcctgaacaa gcggcggaaa gcgaagatta atgatcgaga taaaggtaat gcacccagct
    25021 ggtcatgcgt aatagcactt tgcgcatcac cgagacatgg ctgaaatgat cagagtaaac
    25081 cgccacctgg gcgtaaatac cgtctggtaa agcgctcaaa tcagtgtgct catccagttc
    25141 aatggtcgcg ataacgccat ctgaacccgg cgctgtgttt aacgtctgta aggtgccggt
    25201 cgactgataa gctccgccgg gaacggctgg actaatggct gccagcttac cgctgaatac
    25261 cttacctggc agagcattaa acaccacttc cgcatcgtcg ccaggagcca gtcgcagcaa
    25321 ggagttctga cggaactgcg ccacgatttg tcgtttctga tcgggtataa acaccatcac
    25381 cggacgtagc ggcagcgacg cggcataggt acccggacga atcagtacct gggtcacata
    25441 accatcgctc ggcgcacgca ctatcgtctg ctcaaggtta tattttgctt ccgcgagctg
    25501 tgctttcagg ctggcgattt gagaatgttc acccaacacc aggctatcca gctggctctg
    25561 gatctgtttt tgttccgccg ccgatgactt cacggaggct tcctgcgcca gataattttg
    25621 ccgcgccaca tcgatatcgc gttctgaaaa cgggtttact ttcgcctggc tgccacgtgc
    25681 gtaacgctta tactctttag cgaatttatc ccgcgtggcc tttgcctgct gagtattcgc
    25741 cgccatctca tctaactctg cgcccaacgc ccgctgttta tgttctgcgg taacgatatc
    25801 cgccatcagc cgatccaccc gcgcctgata acgcgtcggg tccagtcgaa atagcacctc
    25861 acctttttta atcagcgtat ttttcttatc cgtcacttcg atcaccacgc ctgtcacctg
    25921 tgggacaaca ggaatagaaa taaccgcttt ttgcgctttg aaggtatacg gatggttata
    25981 gttcattaac agaattaaac cactgacaat aaatatgcca cccagggccg ctgtgggaat
    26041 ggtccattta tttacaggaa ttttgaagat cttaaacatt gcccatgcaa aagccacata
    26101 ggtcaaaata atcagtaaat ccataagtat tactcggcag aagaggattt gatgtcggcg
    26161 agctgtttct ccagcccggc aatacgctgt tgtagttgca caacggagga gtcatggttt
    26221 tgcattcccc atccccgctc cgggcggtaa agcgtggccc aaatccacaa aaatggccag
    26281 ataacatgca gcgtaaaaag actcacccaa ccagcaacat gaatggcgtc ggcatgagga
    26341 tgattacgtt ttttagcaat caggtaggga atatcatgta aaataataat cccatagaat
    26401 atcaccagaa atacaaaaat aagcactccc agtgcaaaat aatccagaaa catatttccc
    26461 tcagagaata taaaaaagaa caattaacgc atattatgaa atcccatgat gcagtgcaat
    26521 ttccttattt atataacggc atattgtttt ttatatactt tctgaagtgt gctgcgtggc
    26581 ttttcaacag gtcgaggttt atcatattga aataatggaa ctattgacca agctaatgaa
    26641 taactccagt aaaacatcca cagtacagat aaagcgtatt aaaccttcaa ttatctaccg
    26701 tttattgctg attggcctcg gaacgccaat ggtgatttac ggcctggttt gcccgctcac
    26761 catcgaaacg cgagagtaaa ctcctggcaa aacacgcccc agaaacgcac ttctgttggc
    26821 gtagtctggg ttattgcgca cgtaggttta aaacgtaaat tgtaacaacg tgcgctttgt
    26881 ttatgccgga tgcggcgtgt acgccttatc cggcctacgg tcaggttccc gtaggcatga
    26941 taagacgcgt aagcgtcgca tcaggcaatg aatacccaat gcgaccagct tcttatatca
    27001 gaacagcccc aacggtttat ccgagtagct caccagcagg cacttggttt gctggtaatg
    27061 ctccagcatc atcttgtggg tttcgcgacc gatacctgat tgtttgtagc caccaaacgc
    27121 agcatgtgcc gggtaagcgt gatagcagtt ggtccacacg cgcccagcct gtatgccgcg
    27181 ccccatctta taggccagat taccgttgcg gctccagacg cccgcgccca ggccatattg
    27241 cgtatcgttc gccagctcca gcgcttcttc catcgttttg aaggtggtca ccgccagcac
    27301 cgggccaaaa atctcctcct ggaacacccg catattgttc tgaccaaaca gaatcgtcgg
    27361 ttcgaggtag tagccgtctt tcagttcacc ttccagcagc ttgcgccgcc cgccggtgag
    27421 cacgtctgcg ccctcttttt taccgatatc aatgtagttg aggatggttt ccagttgccc
    27481 gtgagaaacc tgcgcgccca tttgcgtcac gctgtcgagt gggttaccgc tgcgaatgct
    27541 ttcgacacgg cggatggcgc gttccataaa gcgttcgtag attgactcct gcactaaggc
    27601 acgactcgga caggtgcaaa cttcaccctg gttaaaggca aacagtgcaa agccttccag
    27661 cgctttatcg aaaaaggcat cttcttcatc catcacatca gcaaagaaga tatttggcga
    27721 tttgccgccc agctccagcg tcaccggaat aatgttttgc gtggcgtatt gcataatttg
    27781 ttggcccact tccgttgagc cggtaaacgc cactttggcg atgcgtttcg aggtcgccag
    27841 atattcgcca atttccccac ctgcgccgtt gaccacgttc accacgcccg gcggcagtaa
    27901 atcaccgacg atttccatta gcagcagtac agaaagcggg gtaagacgtg cgggtttcag
    27961 caccacacag ttgcccgccg ccagcgcggg agccattttc cagctcgcca tcagcagcgg
    28021 gaagttccac gggataatct gccccaccac gcctaacggt tcatggaaat gataggccac
    28081 ggtttcgcta tcaacttcac tgatcccacc ttcctgcgcc cgaatacacg aggcgaaata
    28141 gcgaaaatgg tcaatcgcca gcggcacgtc ggcagcactg gtttcgcgaa tgggtttgcc
    28201 gttatcccag gtttcagccg tcgctaacag ctcaaggttt tgttccattc gatcggcaat
    28261 cttaaacaga atcgccgcgc gatcctgcac cgaggtgtgc gcccatttat ctttcacttt
    28321 gtgcgcagca tccagcgcca gatcgatgtc tcgtttgccc gaagacgcca cttcgcacag
    28381 cagctgcccg gtcaccggcg tcagattctg gtaatactcg ccgtcggcag gggctaccca
    28441 ttcgccgcca ataaagttgt catagcgggc ttttaacttg agggggaaac catactcgcc
    28501 gggcttaatc tgtgctgaag ggggattatt ggtcatgatc gtctccttgc ggtgtgaggt
    28561 ataacaaggg tagacgtgac tggcgaaatc ttcgccagtc ggtaacagct ttacgacagc
    28621 tatcacgaat ttacgggcaa gggttacaga gatagttgaa aaagcggctg acaatttgcc
    28681 agccgttgtg gaaatgatga ttacatcgct gcgcgataaa tcgccacaat ttcttcgtga
    28741 gttgcctgga tcgggttagt aaaaccacag gcatctttca gggcattagt cgccagaacc
    28801 gcgaaatctt cttctttcac gttcaggtcg cgtaggcctg ccgggatatc cactttcttc
    28861 gccagttcac ggatggcgtt aatgcaggct tcagcacctt ccgcatcgtt tttacctgtc
    28921 acgttcacgc ccattgcagc ggcacagtca cgcagacgtg cggcggcgac tttgctgttg
    28981 aatacctgaa catgcggcag caaaacggcg ttacatacac cgtgtggcag gttgtagaaa
    29041 ccgcccagct ggtgcgccat cgcatgaaca taacccagag aagcattatt gaacgccata
    29101 ccggcgagga actgggcata agccattgct tcacgcgctt tcgcattact gccatcttca
    29161 acggctaacg gcaggttttc ggcaatcatg gtcacggctt tcagtgcaca agcgtcagtg
    29221 atcggcgtgg cggcaataga aacatacgct tcgatagcgt gcgttaaggc atccataccc
    29281 gttgcggcgg tcagtgactt cggcatacca atcatcagag aggagtcatt gacagaaagc
    29341 agcggagtga catgtttatc aacaatcgcc attttgatat gacgcgcttc gtcagtgatg
    29401 atgcagaaac gggtcatttc agaggccgta cccgccgtgg tattgatggc gatcatcggc
    29461 agctgcggtt ttgcagagcg gtcaacgcct tcgtaatcgc gaatatcgcc gccattggct
    29521 gccaccagcg caataccttt tgcgcagtcg tgtggagaac caccgcctaa ggagatcacg
    29581 ctatcgcaat tattttcttt aagtaatttc aaacctgcgg cgacgttttc cgtggtgggg
    29641 ttaggttggg tgccatcata aataacgcta aaaatattgc gttcttccag tgctttttgc
    29701 acatcacccg ccatacctaa tttcgttaac atactgtcag tgacaattaa ggtacgggta
    29761 aatccataat ctgccatcat attcattgca tcagtcaatg aatcagcgcc gatgacattc
    29821 acagaaggaa taaagaaagt tgaagatgcc ataacacact cctgaaagtg taaaaaaggg
    29881 tgggcagctt
//
LOCUS       DAZGJW010000050        29743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZGJW010000050 DAZGJW010000000
VERSION     DAZGJW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.050"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            115..498
                     /gene="traM"
                     /locus_tag="ACHKIK_002217"
     CDS             115..498
                     /gene="traM"
                     /locus_tag="ACHKIK_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO9083381.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDDE"
     gene            685..1374
                     /gene="traJ"
                     /locus_tag="ACHKIK_002218"
     CDS             685..1374
                     /gene="traJ"
                     /locus_tag="ACHKIK_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HFO9083382.1"
                     /translation="MYPMDRIQQKHARQIDLLENLTAVIQDYQNPACIRDETGKFIFC
                     NTLFHESFLTQDQSAEKWLLSQRDFYELISVTEMEAYRNEHTHLNLVEDVFIQNRFWT
                     ISVQSFLNGHRNIILWQFYDAAHVRHKDSYNQKTIVSDDIRNIIRRMSDDSSVSSYVN
                     DVFYLYSTGISHNAIARILNISISTSKKHASLICDYFSVSNKDELIILLYNKNFIHYL
                     YEKAMCIINTR"
     gene            1473..1868
                     /gene="traY"
                     /locus_tag="ACHKIK_002219"
     CDS             1473..1868
                     /gene="traY"
                     /locus_tag="ACHKIK_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-bindin protein
                     TraY"
                     /protein_id="HFO9083383.1"
                     /translation="MKRFGTRSATGKMVKLKLPVDVESLLIEASNRSGRSRSFEAVIR
                     LKDHLHRYPKFNRAGNIYGKSLVKYLTMRLDDETNQLLIAAKNRSGWCKTDEAADRVI
                     DHLIKFPDFYNSEIFREADKEEDITFNTL"
     gene            1901..2266
                     /gene="traA"
                     /locus_tag="ACHKIK_002220"
     CDS             1901..2266
                     /gene="traA"
                     /locus_tag="ACHKIK_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFO9083384.1"
                     /translation="MNTVLSVQGASAPVKKKSFFSKFTRLNMLRLVRAVIPVAVLMMF
                     FPQLALAAQGAGQDLMQSGNSTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFL
                     AGFAIISVFIAVGMAVVGL"
     gene            2281..2592
                     /gene="traL"
                     /locus_tag="ACHKIK_002221"
     CDS             2281..2592
                     /gene="traL"
                     /locus_tag="ACHKIK_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFO9083385.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            2614..3180
                     /gene="traE"
                     /locus_tag="ACHKIK_002222"
     CDS             2614..3180
                     /gene="traE"
                     /locus_tag="ACHKIK_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFO9083386.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            3167..3895
                     /gene="traK"
                     /locus_tag="ACHKIK_002223"
     CDS             3167..3895
                     /gene="traK"
                     /locus_tag="ACHKIK_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO9083387.1"
                     /translation="MRKNNTAIIFGSLFFSCSVMAANGTLSPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYKSLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPVGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            3895..5322
                     /gene="traB"
                     /locus_tag="ACHKIK_002224"
     CDS             3895..5322
                     /gene="traB"
                     /locus_tag="ACHKIK_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFO9083388.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGALYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATVEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKNGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            5312..5899
                     /gene="traP"
                     /locus_tag="ACHKIK_002225"
     CDS             5312..5899
                     /gene="traP"
                     /locus_tag="ACHKIK_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFO9083389.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYAVILPLATMALLA
                     LLVLWKDNTTPGKLLVQEIEFVRGRTPAGLYPVRYCSNSFGSDLAMPPSVTCGFRRGD
                     AAEYARETDQSLMQLFTALWATLALMYVSLAVISGKYPMRPGKMKCVRVATADEHLKE
                     VLTEDASLPEKIRKCHVYFPDDRTNRNNGDKNEHT"
     gene            5886..6206
                     /locus_tag="ACHKIK_002226"
     CDS             5886..6206
                     /locus_tag="ACHKIK_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFO9083390.1"
                     /translation="MNIRNINVITDRSVPDKSVSDDFMHAVLSNCTTRIVLPAPKKFG
                     SESLSHNFNMAAVGVMKKGMSMNLFSSPGHIVVAGQSGDGKSVPVKELIDRIIQKYKE
                     PFDE"
     gene            6199..6450
                     /gene="trbG"
                     /locus_tag="ACHKIK_002227"
     CDS             6199..6450
                     /gene="trbG"
                     /locus_tag="ACHKIK_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HFO9083391.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRNTVFENPDIVKTCTVRDLKNDFVNCDEKGEEQ"
     gene            6447..6962
                     /gene="traV"
                     /locus_tag="ACHKIK_002228"
     CDS             6447..6962
                     /gene="traV"
                     /locus_tag="ACHKIK_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFO9083392.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKMEQPADAKPVAASLPRLAEGNFRTTSVQAVAETTPSRSRPAVTGLPEQKLLAPR
                     PLFTAAREVKTIVPVSSVAPVTPPRPLRTGEQMAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            7097..7318
                     /gene="traR"
                     /locus_tag="ACHKIK_002229"
     CDS             7097..7318
                     /gene="traR"
                     /locus_tag="ACHKIK_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFO9083393.1"
                     /translation="MSDEADEAYSVTEQMTMTGINRIRRKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            7311..7784
                     /locus_tag="ACHKIK_002230"
     CDS             7311..7784
                     /locus_tag="ACHKIK_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083394.1"
                     /translation="MHKSEAEKIDSILNEHQQIYRRHNRIDNILAFCACIAWVPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFTEGIKSE"
     gene            7864..8079
                     /locus_tag="ACHKIK_002231"
     CDS             7864..8079
                     /locus_tag="ACHKIK_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083395.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIESHGGRG
                     FGNTGECIEVKTNKPLNNVMDTLNGGQ"
     gene            8107..8469
                     /locus_tag="ACHKIK_002232"
     CDS             8107..8469
                     /locus_tag="ACHKIK_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083396.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMLICGLFYNDYLEQQPATPEQLTEI
                     TQDTPCAAETFKEAIKSDTSDYQPEPLSLGKAKKLASACRERNEMAEVKRVRENERNK
                     IREKQLQALNDAHSAKEH"
     gene            8595..11225
                     /gene="traC"
                     /locus_tag="ACHKIK_002233"
     CDS             8595..11225
                     /gene="traC"
                     /locus_tag="ACHKIK_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFO9083397.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAVRFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKKKQARIDDVVDFLKNARDNDQYVESPTIRSRLDEMI
                     VLLDQYTANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            11222..11608
                     /gene="trbI"
                     /locus_tag="ACHKIK_002234"
     CDS             11222..11608
                     /gene="trbI"
                     /locus_tag="ACHKIK_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFO9083398.1"
                     /translation="MTTTQKTTDVTARRRSHWWWTVSGCLAMVLLNAAVSYGIVRLNA
                     PVTAVFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            11605..12237
                     /gene="traW"
                     /locus_tag="ACHKIK_002235"
     CDS             11605..12237
                     /gene="traW"
                     /locus_tag="ACHKIK_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFO9083399.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQVSIPEM
                     QKALDSRIYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            12234..13226
                     /gene="traU"
                     /locus_tag="ACHKIK_002236"
     CDS             12234..13226
                     /gene="traU"
                     /locus_tag="ACHKIK_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFO9083400.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            13253..13561
                     /locus_tag="ACHKIK_002237"
     CDS             13253..13561
                     /locus_tag="ACHKIK_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083401.1"
                     /translation="MEKKLSSKYIHMYMSISRVFIRLTGNVLKLFPFAMVICPVVWFY
                     LCPEHIISDIVNDWQDADTLEKILLVKLYLKTVLILTVVSVVVLSLSSDKFMSVKRRK
                     "
     gene            13624..14142
                     /locus_tag="ACHKIK_002238"
     CDS             13624..14142
                     /locus_tag="ACHKIK_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083402.1"
                     /translation="MSFLSFLRKISNKEKEIFISEERFFSLLERVSAFDVVREWAENG
                     GAYYPLLECRRLTDIEDVRKSEDILNRLEFIDRRFREFISIMSEYNRNDKSKNRWERE
                     LSEYPPEKIYQVRREGNHLSLKNYGDDFTLDRDTAVATVQITVSRKCITQEEMDELYA
                     LSERLKEKCAGN"
     gene            14169..14258
                     /locus_tag="ACHKIK_002239"
                     /pseudo
     CDS             14169..14258
                     /locus_tag="ACHKIK_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
     gene            14350..14517
                     /locus_tag="ACHKIK_002240"
     CDS             14350..14517
                     /locus_tag="ACHKIK_002240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083403.1"
                     /translation="MMEKEQKQCGLQLPLHQDVSLPVPRDTFSMETAEQYRDWQLIAA
                     SLRKKINGERS"
     gene            complement(14514..14753)
                     /locus_tag="ACHKIK_002241"
     CDS             complement(14514..14753)
                     /locus_tag="ACHKIK_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083404.1"
                     /translation="MNNNTSEDRPDFSFSYMDNQRADEIKALKILLFSIIRNMPHDTA
                     ISIINNIVNTDNNEALNRLCLEITDAMLNTTRIVD"
     gene            14946..15584
                     /gene="trbC"
                     /locus_tag="ACHKIK_002242"
     CDS             14946..15584
                     /gene="trbC"
                     /locus_tag="ACHKIK_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFO9083405.1"
                     /translation="MNKKIYILFALTAGLFISVHASENVNTTENRQFLKQQETLSQQL
                     REKPDTQLQAWAEQQVRANPLQHSDNQFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKATADAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGHDIIRGNLRVRQALEKVAATGDCRQVAQGLLDGTGDKSQ"
     gene            15581..15952
                     /locus_tag="ACHKIK_002243"
     CDS             15581..15952
                     /locus_tag="ACHKIK_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083406.1"
                     /translation="MSLPAGYYRIDPDIRALVAAMNVHGFRTYASCQGHGFPVTKLPP
                     YIAFVCPVKKAALLEQRLRQDAESMMPRLLWGWSVGASFNSDLQLCFRLQPEGPHHWY
                     HRYCRRSLRADFRTLVRLLNP"
     gene            15977..16399
                     /locus_tag="ACHKIK_002244"
     CDS             15977..16399
                     /locus_tag="ACHKIK_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO9083407.1"
                     /translation="MALTKKQREKLRMKFGGRCAYCGCELPEKGWHADHVQAVLRKSE
                     QCMKAAEKRIFRLKSTGDVFRPEADCPENLVPACAPCNLLKTTYSLEMFRTQVSLQVE
                     RGRRSSVNFRTAERFGLIQVVNKPVVFWFEQYQRESEV"
     gene            16396..18231
                     /gene="traN"
                     /locus_tag="ACHKIK_002245"
     CDS             16396..18231
                     /gene="traN"
                     /locus_tag="ACHKIK_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFO9083408.1"
                     /translation="MKRLLPLLLGLAVSVAQADSNSDYRAGSDFARQIQGQGTGSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDTMVEEYCTRT
                     AAMTGHYENTTEIKQIVIESSAIQFTENNNSFSGEFKVSTRGKVIAASAVYSWNKRLA
                     FSNPSWFMKVITPFGLISMNDSKGSVTLSSGLDITDGQSLLFSITNRHNKPTVSSIWY
                     SSNMRYRFTITLTIQVPSRKWVPDVIWTESCPFSKELGKLTKTECTEAGGNRTLVKDG
                     QSYSMYQSCWAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATY
                     SCESRTSGKVMVCGGDVFCLDGECDKAQSGKSNDFAEAVSQLAALAAAGKDVAALNGV
                     DVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLTVSVGE
                     FCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGITVDELQ
                     KIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            18258..18515
                     /locus_tag="ACHKIK_002246"
     CDS             18258..18515
                     /locus_tag="ACHKIK_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFO9083409.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTAGEML
                     AACFVIFYSGVLLFILYFCFSALTDLLKTDERKNDERNEDE"
     gene            18508..19251
                     /gene="traF"
                     /locus_tag="ACHKIK_002247"
     CDS             18508..19251
                     /gene="traF"
                     /locus_tag="ACHKIK_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFO9083410.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            19265..19630
                     /locus_tag="ACHKIK_002248"
     CDS             19265..19630
                     /locus_tag="ACHKIK_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083411.1"
                     /translation="MSFISLWPLLLAQVTVMSLTVIFLAADRRFFSWDCFVVCTLSMM
                     MAEGIVIMKYELWHIPPVLLGLAGGLSWSLLLLHTGYFSDISEGVHKRLWLAVATVFS
                     GAGMIIFYWLLHASENIIK"
     gene            20009..20290
                     /gene="traQ"
                     /locus_tag="ACHKIK_002249"
     CDS             20009..20290
                     /gene="traQ"
                     /locus_tag="ACHKIK_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001732192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFO9083412.1"
                     /translation="MRKYKFSLPRLDITGMWVFSLGVWFHIVARLVHSKPGMAFFLAE
                     LIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMEGQQEGGHVSH"
     gene            20277..20822
                     /gene="trbB"
                     /locus_tag="ACHKIK_002250"
     CDS             20277..20822
                     /gene="trbB"
                     /locus_tag="ACHKIK_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFO9083413.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAAQPAQPAA
                     GTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKLLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGGEKGAK"
     gene            20812..21126
                     /gene="trbJ"
                     /locus_tag="ACHKIK_002251"
     CDS             20812..21126
                     /gene="trbJ"
                     /locus_tag="ACHKIK_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFO9083414.1"
                     /translation="MRNKQVVLLIAGISGIATGIIVSLNIPFIRQGLFYPASPVEIVV
                     SLCLTFSVSVVFFVGAIVGWISVSEIYYSRMTGLNESSEISEETYDDRKQKESGIQNQ
                     KN"
     gene            21080..21472
                     /gene="trbF"
                     /locus_tag="ACHKIK_002252"
     CDS             21080..21472
                     /gene="trbF"
                     /locus_tag="ACHKIK_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFO9083415.1"
                     /translation="MTENKKNQEFKIRKIKRNIERSCDNAIKYFELFVVFFVAGLLLW
                     HGMHAFFSAGIDSWKADPELNNLIYMWNILMYAIPYTLYALAAGFLVTSFLSPMYELI
                     FGNIMIFQLKRRMRRENTLREGSNNASH"
     gene            21459..22832
                     /gene="traH"
                     /locus_tag="ACHKIK_002253"
     CDS             21459..22832
                     /gene="traH"
                     /locus_tag="ACHKIK_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFO9083416.1"
                     /translation="MLRIKPLFILCAALLTAIPTASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAIFQSQVQVQQDALLVVERQMSYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            22829..25654
                     /gene="traG"
                     /locus_tag="ACHKIK_002254"
     CDS             22829..25654
                     /gene="traG"
                     /locus_tag="ACHKIK_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFO9083417.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALALSVVAV
                     AFMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TINALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTSTRLSGGMY
                     ADAHAEWGVKPKILGVGGGVGIRGGFKGSLDATDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSIHGEENIVDRQYSELKNNHKQEEIQQNNRYNEENKR
                     QKLMPTPSEDALKQMVDDKKERLKGPL"
     gene            25651..26160
                     /gene="traS"
                     /locus_tag="ACHKIK_002255"
     CDS             25651..26160
                     /gene="traS"
                     /locus_tag="ACHKIK_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HFO9083418.1"
                     /translation="MITHYDIKMEMQKLKEVLSVEGVNIPSLLQVIKPGTYVFLWVLL
                     WPTFLRLVSVKSDVRDVGFDICASGMMGFLLFVAITNGMMLYLAIPDSFRKDSKIINF
                     MYSKSKTYILLFLIVFSMVSFMHSILYVFALMITFILFFLVYTIDINRYNLSAIASVI
                     GLFKKESVS"
     gene            26174..26905
                     /gene="traT"
                     /locus_tag="ACHKIK_002256"
     CDS             26174..26905
                     /gene="traT"
                     /locus_tag="ACHKIK_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFO9083419.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LKPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDTAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            27108..27845
                     /locus_tag="ACHKIK_002257"
     CDS             27108..27845
                     /locus_tag="ACHKIK_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083420.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILEKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPKKLAEVSREIIVASAQEIAWTPGSSRIDGHQGENITWSLSSQS
                     PADKYDLINETSRVSPDSGRFYEPDTVLDRRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRAAGGYHHRHTGYTWSVSVN"
     gene            27896..>29743
                     /gene="traD"
                     /locus_tag="ACHKIK_002258"
     CDS             27896..>29743
                     /gene="traD"
                     /locus_tag="ACHKIK_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO9083421.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLI
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKEVV
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKVLNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEGVTQA"
BASE COUNT         7721 a         6656 c         7913 g         7453 t
ORIGIN      
        1 ctttttttta taggatacag ccaggggcgc tgctagcggt gcgtccctgt ttgcattatg
       61 aattttagtg tttcgaaatt aactttattt tatgttcaaa aaaggtaatc tctaatggct
      121 aaggtaaacc tgtatatcag caatgatgcc tatgaaaaaa taaatgcgat tattgagaag
      181 cgtcgacagg aaggggcaag ggaaaaagat gtcagttttt cagcaacagc ttcaatgctt
      241 cttgaactgg ggcttcgtgt atatgaggct cagatggagc gtaaagagtc tgcatttaat
      301 cagactgagt ttaataaatt gcttcttgaa tgcgttgtaa aaacacaatc atcagtagcg
      361 aaaattttgg gtattgagtc tctcagtcct catgtctccg gaaatccaaa gtttgaatat
      421 gccaatatgg ttgaagatat ccgggagaag gtatcatctg agatggaacg attttttcca
      481 aaaaatgatg atgaataaaa gaaatttgac ttcgttcaaa tatcagagtt tttatgattt
      541 aaaaaggtga cagtacgaaa gataattagt atattaatta cgtggttaat gccacgttaa
      601 aatttgaaat tgaaaatcgc cgatgcaggg agaggacaac tccccgcatc gactgtccat
      661 agaatccttt gtgaggaggt tcctatgtat ccgatggatc gtattcaaca aaaacatgct
      721 cgtcaaatag atctgctgga aaatctgacg gcagttattc aggattatca aaatccagcc
      781 tgtatcaggg acgaaactgg aaaatttatt ttttgcaata cgctgtttca tgagtcattt
      841 cttacacaag atcaaagtgc tgaaaaatgg cttctgtcgc agagagattt ttatgaattg
      901 atctctgtca cagagatgga agcatacagg aatgagcata cgcatcttaa tcttgtagag
      961 gatgttttta ttcagaacag attctggaca atatctgtcc agtcatttct taatggacac
     1021 agaaatatta ttctgtggca attttatgat gctgctcatg ttcgtcataa agacagttat
     1081 aatcaaaaaa cgattgtcag tgatgatatc agaaatataa tcagaagaat gagtgatgat
     1141 tcttctgtat catcatatgt aaatgatgta ttttacttat atagcaccgg aatcagtcat
     1201 aatgctatag caagaatatt aaacatatcc atctccacat caaagaaaca cgcatctctg
     1261 atatgcgact acttctctgt ttctaataaa gatgagttaa tcatcttact ctacaataaa
     1321 aactttattc attatttata cgagaaggct atgtgtatca taaatacgcg ttaataaagt
     1381 gttaataaaa tatagacttt ccgtctattt accttttctg attattctgc aaacataagt
     1441 ggtaaccaga agataaacag cgggaggtgt tattgaaaag atttggtaca cgttctgcaa
     1501 caggtaagat ggtaaaacta aaattacctg tagatgtgga aagtctatta attgaggcaa
     1561 gtaacagaag cggaagaagt cgatcgtttg aggcagtaat aagacttaaa gatcatcttc
     1621 accgctatcc aaagtttaac agggcaggga atatctatgg taagtcgctg gttaagtatc
     1681 tgacaatgcg tctggatgat gaaactaacc agctacttat tgcagcaaaa aatcgtagtg
     1741 gatggtgtaa aacagatgag gctgcagaca gagttattga tcatttgatc aagtttcctg
     1801 atttttataa ctcggagata ttcagggagg cagataaaga ggaagatata acatttaata
     1861 cactctagtt ttattcattt atccgaaatt gaggtaactt atgaatactg ttttaagtgt
     1921 tcagggtgct tctgcgcccg tcaaaaagaa gtcgtttttt tctaaattca ctcgcctgaa
     1981 tatgcttcgc ctggttcgtg cagtgatccc ggttgctgtt ctgatgatgt tcttcccgca
     2041 actggcactg gccgcccagg gggcaggtca ggacctgatg cagagtggta actccacggt
     2101 taaggcgacc ttcggtaagg actccagtgt tgttaaatgg gttgttctgg ctgaagtcct
     2161 ggtcggtgcg gtcatgtaca tgatgaccaa aaacgtcaag ttcctggccg gttttgccat
     2221 catctctgta tttattgctg tgggtatggc cgtcgttggc ctctgacagg aaataaaatg
     2281 atgtcgggag acgagaataa acttaagaaa tatcgtttcc cggaaacact gaccaaccag
     2341 agccgctggt ttggcctgcc actggatgaa ctgatccccg cagcaatctg tattggctgg
     2401 ggtatcacaa catcgaaata tctgttcggt attggtgcag cggttctggt ttatttcggg
     2461 attaaaaaac tgaaaaaagg gcggggcagt tcctggttac gtgacctgat ttactggtat
     2521 atgccaacag ccctgctgcg cggtattttt cataatgttc ccgattcgtg tttccggcag
     2581 tggattaaat agaactgata ccaggattgt tatatggaac acggtgcccg tttaagtacc
     2641 agtcgtgtaa tggccatcgc ctttatattt atgtcagtgc ttattgttct cagcctctct
     2701 gttaacgtca ttcagggggt gaataactac cgtcttcaga atgagcaacg cactgccgtg
     2761 accccaatgg gatttaatgt gccctttgcc gtgtcacaga acagtgccga cgcctcttat
     2821 ttacagcaga tggcgctgtc atttattgcc ctccgtctga atgtttcatc agaaaccgtc
     2881 gatgcctcac atcaggcgct tctgcaatat atccgcccgg gcgcacagaa ccagatgaaa
     2941 gttattctgg ctgaagaagc gaagcgtatt aaaaacgata acgtgaactc agcctttttc
     3001 cagaccagtg ttcgtgtctg gcctcagtat ggccgtgtgg aaattcgtgg tgtgcttaaa
     3061 acctggattg gtgattcaaa acctttcact gatatcaaac attacatcct tattctgaag
     3121 cgggaaaacg gggtgacctg gctggataat ttcggggaaa cagacgatga gaaaaaataa
     3181 tacggcaata atattcggca gcctgttttt ttcctgcagc gtgatggccg caaacggtac
     3241 actgtccccc accgtggtgc caatggtgaa cggtggtcag gccagtattg ccatcagcaa
     3301 taccagcccg aatctgttta ccgttcccgg tgaccggatt atcgccgtga acagtctgga
     3361 tggtgccctg accaataatg agcagaccgc ctccggcggt gtggtggtgg ccaccgtcaa
     3421 caaaaagccc tttacgttca ttctggaaac agagcgtggt ctgaaccttt ccattcaggc
     3481 tgttccccgt gaaggcgcgg ggcgtaccat tcagctggtc agtgacctgc gcggaaccgg
     3541 agaagaagcc ggtgcgtggg aaacgtccac gccttacaaa tccctgcttg tgaccatcag
     3601 ccaggccgtc cgtggcggaa aattgcccgc aggctggtat caggtcccgg tgacaaagga
     3661 aaccctgcag gccccggtgg ggctgtcttc agtggcagat gccgtatgga cggggaatca
     3721 cctgaagatg gtccgctttg ccgtggaaaa taaaacgctg tctgccctga atatccggga
     3781 aagtgacttc tggcagccgg gaacccgtgc cgtgatgttc agccagcctg ccagccagtt
     3841 actggcaggt gcgcgcatgg atgtgtatgt catccgtgac ggggagggca actgatggcc
     3901 agtatcaata ccattgtgaa acgcaagcag tacctgtggc tggggattgt ggttgtcggt
     3961 gcagcctccg cgattggtgg tgcactgtat ctgtctgatg tggacatgtc cggtaacggt
     4021 gaggcggtgg ctgaacagga gcctgtgccg gatatgaccg gtgtggtgga tacgaccttt
     4081 gatgacaagg tgcgtcagca tgccaccaca gagatgcagg tgacggcagc gcagatgcag
     4141 aagcagtatg aggaaatccg tcgtgaactg gatgttctga acaaacagcg cggtgatgac
     4201 cagcgtcgta ttgaaaagct gggacaggac aatgccgccc tggcagagca ggtaaaagcc
     4261 ctgggtgcta atcccgtcac ggcgaccgtt gagcctgtac cgcagatgcc tgcctcaccg
     4321 cccggcccgg aaggcgaacc acagccagga aacacccccg tatccttccc gccgcagggc
     4381 agcgttgctg ttccaccgcc gacggcgttt tatcccggga atggtgtcac gccaccacca
     4441 caggtgacgt accagtctgt gccggtgcct aaccggatac agcgtaaggt gtttacccgt
     4501 aatgagggaa aacagggacc atcgctgccg tacattccgt caggaagttt tgcgaaagcc
     4561 atgctgattg aaggggcgga tgccaatgcc tcagtcaccg gtaatgaatc cacggtgccg
     4621 atgcagctgc gtatcaccgg tctggtggaa atgccgaaca gcaagacgta tgacgcaacg
     4681 ggatgttttg tggggctgga agcctggggg gatgtctcca gtgaacgtgc tattgttcgc
     4741 acccgtaata tcagctgcct gaagaatggc aaaaccattg atatgccgat taaggggcat
     4801 gtcagcttcc ggggtaaaaa cggtatcaag ggcgaagtgg tgatgcgtaa cggcaaaatc
     4861 ctcggctggg catggggtgc gggatttgtt gacggtatcg gtcagggaat ggagcgcgcc
     4921 tcccagccgg ctgtcgggct gggggccact gccgcttacg gggccggcga tgtcctgaaa
     4981 atggggattg gtggcggcgc atcgaaagcc gcacagacgc tcagtgacta ctacatcaaa
     5041 cgtgccgaac agtatcaccc ggtgataccg attggtgcgg gcaacgaagt gaccgtggtg
     5101 ttccaggacg gcttccagct gaaaactgta gaagagatgg cgctggagcg cacgcagaac
     5161 agagcagaag aagacaatcc ggaaagtccg gttcctgttc cgccatcagc tgaaagtcat
     5221 cttaacggct ttaatactga ccagatgctg aagcagctgg gcaacctgaa tccgcagcag
     5281 tttatgtccg gcagccaggg agggggcaac gatggcaaat aatatgtcgt cccgtcaggc
     5341 gtgccatgcc gcccggtatg ttgtcgcccg ggttctgcga ggactgttct ggtgtctgaa
     5401 atacgccgtc attctgccgc tggccacaat ggctctgctg gcgttactgg ttttatggaa
     5461 ggacaacacc acaccgggga aattgctggt tcaggagatc gagtttgtaa ggggcagaac
     5521 gccagccggg ctgtatccgg tccgatactg ctcgaacagt tttggttccg atttagcgat
     5581 gcctccgtct gtaacgtgcg gttttcgccg gggggatgct gcagaatatg ccagggagac
     5641 tgatcagtct ctgatgcaac tgttcaccgc attatgggca acgctggcac tgatgtatgt
     5701 ctcgctggcg gtgatctccg gaaaatatcc gatgcgaccc ggaaaaatga aatgtgtccg
     5761 ggtggcaact gcagatgaac atctgaagga ggttcttacg gaagatgctt ccctgccgga
     5821 aaaaatcagg aaatgccatg tttatttccc tgatgacagg acaaacagaa acaacggaga
     5881 taaaaatgaa catacgtaat attaatgtta ttactgaccg ctctgtaccg gataaaagcg
     5941 tatcagatga ttttatgcat gctgttctca gtaactgcac cacaagaatc gtacttcctg
     6001 caccgaagaa attcggttct gaatcattgt cgcacaattt taatatggct gctgtcggtg
     6061 tgatgaagaa gggtatgtcg atgaacttat tttcttctcc ggggcatatt gtggttgccg
     6121 gacaatccgg tgacggaaaa tctgttccgg tgaaagaact aatcgacaga attattcaga
     6181 aatataagga acccttcgat gaataagctg gtttctgatg gcagcgtcaa aaaaataaat
     6241 tatccggtgc tgtatgagtc cggaataaca cctccgttat gtgaagtcag tgcaccagaa
     6301 cctgatgcgg ggggaaaacg cattgtggca tatgtttata aatcttcacg gaatacggtt
     6361 tttgaaaatc ctgacattgt aaaaacctgt acggtcaggg atttgaagaa tgattttgtg
     6421 aactgcgatg agaaaggtga ggaacaatga aacagatttc tttatttatt cctctgctgg
     6481 ggaccttatt actttccggc tgtgccggaa ccagtacgga atttgagtgt aacgccacca
     6541 catccgatac ctgtatgacg atggagcagg ccaatgaaaa agccaaaaag atggagcagc
     6601 ctgcagacgc aaagccggtt gcggcatcac tgccgcgcct ggctgaaggg aacttccgga
     6661 ccacctcggt gcaggccgtt gcggagacaa cgccgtcccg cagcaggcct gctgtgacag
     6721 gacttccgga gcagaaactg ctggcaccac gcccgctgtt taccgctgcc cgggaagtga
     6781 aaacgattgt tccggtcagt tcagttgcgc cggtaacacc tccccgtccg ctaagaacgg
     6841 gtgagcagat ggctgcatta tggatagcgc cttatattga taatcaggat gtttatcatc
     6901 agccatccag tgtgtttttt gttattaaac cgtctgcgtg gggaaaacca cgtattaatt
     6961 aactggccct gaagttgtat atcagccggc aatgccggcg ggctttctgt attcaaaatc
     7021 tgcctgtaaa acgagtgccc tgtgcgtgaa aagggatggg cggtagcgtg tcagaacatg
     7081 tcgggaggaa tattccgtga gtgatgaagc cgatgaagca tattcagtga cagaacagat
     7141 gaccatgaca ggaataaacc ggatacgccg gaaaataaat gctcatggta ttcctgttta
     7201 tctctgtgaa gcatgcggaa atcccattcc ggaagcccgg cggaaaatat ttcccggtgt
     7261 gacgttatgt gttgaatgtc aggcgtatca ggaaaggcag aggaaacact atgcataaat
     7321 cagaagctga aaaaatagat tctattctga atgaacatca acagatatat cgcagacata
     7381 accgaataga taacatcctg gcgttttgtg cgtgtattgc atgggttccc tgtattattg
     7441 ctgcgttcgg cgttgatggt atatatctga aaatcctgtt tgcagtcctt gcttgctgtg
     7501 gtgcggtatg cttttttatt ttcttcttca ccctcctgcc ggaatcactc atggtcctca
     7561 gccgtcagag cctgcttcag cttatgcgtc tgactgaaga cgtaccggat gcgagacagg
     7621 aactgcttaa ccgtctgctg tccggtaaaa aactgactgg tcgggatgaa aaggatatcc
     7681 ggcgtctctg gcaggagaaa gtggatgcaa tgcaggagtc tgccacccgt cagcgtgaac
     7741 aggatactat tcggaagttt actgaaggga ttaaatccga atgagaatct gcagtgtttt
     7801 atgacctcat aagttaactg aagggatatt cagtgattaa tatacaagcg gaggtaataa
     7861 agaatgcata cctttatttt tcttgtgctg ataaaaaata ttaacggtgg cggaagctgg
     7921 aatattacac ccatgccaaa tacggacgta tgcgtgcagg ttcttcggac aatcgaatca
     7981 cacggaggca ggggctttgg aaatacaggg gaatgtattg aggttaaaac aaataaacct
     8041 ttgaacaacg tgatggatac actgaatggg gggcaatgag tgcttactgc ttcaaaggaa
     8101 aatgaaatga agtcgatata tttaaaatct gttctggctt ttatatttgt tggtgttatg
     8161 gctatgttga tatgtggtct tttctacaat gattaccttg agcaacaacc tgcaacacca
     8221 gaacaactga cagagattac acaggatacc ccctgtgcag cagaaacatt taaggaagcc
     8281 attaagtctg acacttctga ttatcagcct gaacctttaa gccttggtaa ggcaaaaaag
     8341 cttgcatcag cgtgtagaga aagaaacgaa atggcagagg tgaaacgggt aagagaaaat
     8401 gagcgaaata agatacgtga aaaacaactt caggcattaa atgatgctca ttcagcaaag
     8461 gaacattaat cctggtttcc tgttaaaagc ctccatctct tatttgtgct gtggttttaa
     8521 gaatggcact gcctgatata tccacggtca cagactgata ttttcctttt ttaaacgcac
     8581 taaggaatac tcatatgagt aataacccac ttgaggccgt cactcaggcg gttaactccc
     8641 tcgtcacagc actgaaactg cctgacgaat ccgcaaaggc caatgaagtt ctgggcgaaa
     8701 tgagcttccc gcagttcagc cgtctgctgc cgtaccgtga ttacaatcag gagtccggcc
     8761 tgttcatgaa tgacaccacg atgggcttta tgctggaagc cattcccatc aatggggcga
     8821 atgagtccat tgtggaggct ctcgatcata tgctgcgcac caaactgccg cgcggtattc
     8881 cgttgtgtat ccatctgatg tccagtcagc tggttggtga caggattgaa tacgggctgc
     8941 gtgagttctc ctggtcaggt gaacaggctg tacggtttaa cgccattacc cgtgcctatt
     9001 atatgaaagc ggcagcgaca cagtttccgc tgccggaggg gatgaatctg cccctgaccc
     9061 tgcgccatta ccgggtattt atctcgtact gttctccctc gaagaaaaaa agccgggctg
     9121 acattctgga aatggaaaat ctggtgaaaa tcatccgtgc ctcttttcac ggcgcaaaaa
     9181 tcaccacaca gacagtggat gcacaggctt tcatcgagat tgtcggggag atgattaacc
     9241 ataatccgga ctctctgtac ccgaaaagac gtcagctgga cccgtattct gatctgaatt
     9301 atcagtgtgt agaggacagt tttgatctga aggtccgggc ggattacctg acgttggggc
     9361 tgcgtgagaa cggcaggaac agcacagccc gtatcctgaa tttccatctg gcccgtaacc
     9421 cggaaatcgc cttcctgtgg aacatggccg ataactacag caatctgctg aacccggaaa
     9481 tgtcaatttc ctgcccgttc atcctgacgc tgacactggt ggtggaagac caggtgaaaa
     9541 cccacagcga agccaacctg aagtacatgg acctggagaa aaagtcgaag acctcctatg
     9601 ccaaatggtt tccgtccgtg gagaaagagg cgaaggagtg gggggaactg cgtcagcggc
     9661 tgggctccgg tcagtcctcc gtggtgtcct acttcctcaa catcacagca ttctgcaagg
     9721 acaataatga aacggcgctg gaagtggagc aggacatcct gaacagcttt cgtaaaaacg
     9781 gttttgagct gatttcaccg cgctttaacc acatgcgcaa tttcctgacc tgcctgccct
     9841 ttatggccgg gaaagggctg tttaaacagc tgaaagaggc cggagtggtt cagcgcgcag
     9901 agagctttaa tgttgccaac ctgatgccgt tagtggcgga taaccccctg acaccggcag
     9961 gtctgctggc acccacctac cgtaaccagc tggcgtttat cgatatcttc tttaagggga
    10021 tgaataacac caactacaac atggcggtct gtggaacatc cggggccggt aaaaccgggt
    10081 tgatacagcc acttatccgc agcgtgctgg actccggggg ctttgccgtg gtgttcgaca
    10141 tgggggatgg ctacaagtct ctgtgtgaga acatgggggg cgtatatctg gacggtgaaa
    10201 ccctgcgctt taacccgttt gcgaacatca ctgatattga ccagtcagcg gagcgtgtcc
    10261 gtgaccagtt gtcggtgatg gccagcccca acggtaacct ggatgaagtg catgaaggtc
    10321 tgttgctgca ggcggtcagg gcctcctggc tggctaaaaa gaagcaagcg cgcattgatg
    10381 atgtggtgga tttcctgaaa aacgcccgtg ataacgacca gtatgtcgag tcaccgacca
    10441 tccgcagccg tctggacgaa atgattgtgc tgcttgacca gtacactgcc aacggcactt
    10501 acggccggta ttttaactct gatgaaccgt ccctgcggga tgatgccaga atggtggtgc
    10561 tggagctggg tggactggaa gaccgtccgt ccctgctggt tgcggtgatg ttctccctga
    10621 ttatctacat cgagaacagg atgtaccgca cgccgcgtac tctcaaaaaa cttaacgtca
    10681 ttgatgaagg ctggcgtctg ctggacttca aaaatcgtaa agttggtgag tttattcaaa
    10741 aaggttaccg cacctgtcgc cgtcataccg gtgcctatat caccatcaca cagaacatcg
    10801 tcgactttga ctctgacaag gcatccagcg cagcccgtgc ggcatggggt aactcctcct
    10861 acaaaattat cctcaaacag agtgcgaagg agttcgcgaa atataaccag ttattcccgg
    10921 atcagttcca gccactgcag cgcgacatga ttggtaagtt tggtgcggcc aaagaccagt
    10981 ggttcagttc cttcctgctg caggtggaaa accattcctc ctggcaccgt ctgtttgtgg
    11041 acccgttaag ccgtgccatg tacagctctg acggcccgga ttttgagttt gtgcagcaga
    11101 aacgcagaga gggtctgagt attcatgagg cggtgtggca gctggcgtgg aagaaatcgg
    11161 ggccggagat ggcttcgctg gaggcctggc tggaagaaca tgagaaatac aggagtgttg
    11221 catgaccaca acgcaaaaaa cgactgacgt tacagcccgt cgacgcagcc actggtggtg
    11281 gacggtgtcg ggatgcctgg caatggtgtt actgaacgca gccgtcagtt acggcattgt
    11341 cagactgaac gcaccggtga ctgccgtctt caacatgaaa cagactgtgg atgcattttt
    11401 tgacagcgcc agtcagaaac aactgtcaga agcacagtca aaagctcttt cagcgcgttt
    11461 taacacggca ctggaagcca gcctgcaggc gtggcagcag aaacaccacg cggtcattct
    11521 ggtgtcgcct gccgtggtac agggggctcc ggatatcacc cgggaaatcc agcaggatat
    11581 tgcccggcga atgagagcgg aaccatgaga tgccgggggc tgattgccct gctgatatgg
    11641 gggcagagtg tggccgccgc cgatcttggc acctggggcg atctgtggcc ggtaaaggag
    11701 ccggacatgc tgacggtgat catgcagcgc ctgacggcac tggagcagtc cggtgagatg
    11761 ggccggaaaa tggatgcctt taaggagcgg gtgatccgta acagtctgcg gccccctgcc
    11821 gtgcccggta tcggacgcac ggagaaatac agcagccggt tgtttgaccc gtccgtcaga
    11881 ctggctgcgg atatccggga taacgaaggg cgggtgtttg cccgtcaggg tgaagtgatg
    11941 aatccgctgc agtatgttcc ttttaaccag acgctgtatt tcatcaatgg cgatgatccg
    12001 gcgcaggtgg cctggatgaa acgccagacg ccgcccacac tggagagcaa aattatcctc
    12061 gtgcaggtca gtatcccgga gatgcagaaa gcgctggaca gccgtattta ctttgaccag
    12121 aacggtgtgc tctgccagcg cctcggcatt gatcaggtcc cggcacgggt gagcgccgtt
    12181 cccggcgatc gcttcctgaa ggtggaattt attccggcag aggagggcag aaaatgaagc
    12241 gaagcctgtg gctgctgatg ttattccttc tcgccggtca tgtccctgcg gcgtctgcgg
    12301 attctgcctg tgaggggcgt tttgtaaacc cgatcacgga tatctgctgg agctgtattt
    12361 tcccgctctc gctgggcagt atcaaagtca gtcagggtaa ggtccccgac acggcgaacc
    12421 cgtcgatgcc cattcagatt tgtccggcac cgccgccgct gttcaggcgt atcgggctgg
    12481 ccattggtta ctgggagccg atggcgctga cggatgtcac ccggtcaccg ggatgcatgg
    12541 ttaacctggg cttcagcctg ccggcttttg gtaaaacggc acagggaacg gcgaaaaagg
    12601 atgagaagca ggttaacggg gcgttctatc acgttcactg gtacaaatac ccgctgacgt
    12661 actggcttaa catcatcaca tcgctgggct gtctggaagg tggtgacctg gatatcgctt
    12721 atctttctga aatcgatccc acctggacgg acagcagcct gaccaccatt ctcaatccgg
    12781 aagctgtcat ctttgccaat ccgatagcac agggggcctg cgcagcagat gccattgcca
    12841 gtgcctttaa tatgcctctc gatgttctgt tctggtgtgc cggttcgcag ggcagtatgt
    12901 acccgttcaa tggctgggtg agtaatgagt ccagtccgtt gcagtcctcc ctgctggtca
    12961 gtgaacgcat ggcattcaag ctgcaccgtc agggcatgat tatggaaacc atcgggaaaa
    13021 ataacgccgt ctgtaatgaa tatccgtccc caatcctgcc caaagaacgc tggcgttatc
    13081 agatggtgaa tatgtatccg gacagcggac agtgccatcc gttcgggcgc agtgtgatgc
    13141 gctgggagac gggaaaaaat ccgcccaaca caaagaaaaa cttcggctac ctgatgtggc
    13201 gtaaacgtaa ctgtgtattt ctgtaactta aacaaaaaga ggaatatttt aaatggaaaa
    13261 gaaattaagc agtaaataca ttcatatgta tatgagtata tcccgagtgt tcattcgttt
    13321 gacaggcaat gtattaaaac tgtttccttt tgctatggtg atatgccctg tggtgtggtt
    13381 ttatctttgc cctgaacata ttatctcaga cattgttaac gactggcagg atgcagatac
    13441 actggaaaaa atactgcttg tcaaactgta tctgaagacg gttcttattc tgacagtggt
    13501 aagcgttgtt gtcttgtctc tttcgtcaga taagtttatg tctgttaagc gcaggaaatg
    13561 agtctgtcgg ttctcataaa tataaccgtg tgtgcatatg taaaatataa ataaaagtgt
    13621 gttatgagtt ttttatcatt tttacgaaag ataagtaata aagaaaaaga aatttttatc
    13681 agtgaggagc gttttttctc cctgctggaa agggtgagtg cttttgatgt ggtcagagaa
    13741 tgggctgaaa atggtggagc atattatcct ctgctggagt gccgtcggct tacagatatt
    13801 gaagatgtca ggaaatcaga ggatatactg aaccgactgg agtttattga cagaagattt
    13861 cgggaattta tcagcattat gtctgaatat aatcgtaatg ataaatctaa aaacaggtgg
    13921 gagagggaat tgtctgaata tccaccggaa aaaatatacc aggtccggag agagggaaac
    13981 catctgtcat taaaaaatta cggtgatgat tttactctgg acagagatac tgccgtggct
    14041 acggtgcaga ttactgtcag cagaaaatgc attactcagg aagaaatgga tgaattatat
    14101 gcattgtctg agagactgaa agaaaagtgc gccggtaatt aaattatatc actgtgccgg
    14161 gagatgatat gagccggaaa atatttgtac tttttgccct ggctgcaggg atgcatatca
    14221 ctgttcatgc ctcagacaat gtaaacactc ctgaaaactg acaattcctg aagcagcagg
    14281 aaaatttaag cagacaacta cgcgaaaacg taactgtgta tttttataac actgacaaga
    14341 aaaggataaa tgatggaaaa agaacagaaa caatgtggcc tgcagctccc cttgcatcag
    14401 gatgtatctc tccctgtacc ccgtgacact ttcagtatgg aaacagcaga gcaatacaga
    14461 gactggcaat taattgcagc cagtctcagg aaaaagataa atggggaaag aagctaatca
    14521 acgattctcg ttgtgtttaa catggcgtca gttatctcca ggcataatct attgagcgcc
    14581 tcattattat cagtatttac gatgttatta ataatgctga tggctgtatc atgaggcata
    14641 tttctgatga tactgaataa taatattttt aaagccttta tttcatctgc tctctgattg
    14701 tccatgtaac tgaatgaaaa atcagggcga tcttctgatg tgttattatt cattgtaact
    14761 ccttgttggt atgtttttag cctgtcatct cttgcaatcg atatatcagg ctgaaatatt
    14821 ttaccacaac gcgaccgggc gcgtaatccc ggggttaata attcctgaaa taccgtgaga
    14881 atctggttgt tgatatcaga tgttggttgt ggtgattaat tactgcctgt ttgcagaaga
    14941 attatatgaa caaaaaaata tacattttat tcgccctgac agcaggactg tttatctctg
    15001 ttcatgcttc agaaaatgtg aacacaactg aaaaccgcca gttcctgaag cagcaggaaa
    15061 cactgagtca gcagttacgt gaaaagccgg acacacagtt gcaggcatgg gcagagcagc
    15121 aggtcagggc gaatcccctt cagcattccg ataaccagtt tctcgatgaa ctggtccgta
    15181 aacagcaggc ttcgcaggac ggaaaacccc ggcagggtgc cctgtatttt gtgtcgtttt
    15241 ccattcccga agaggggctg aaacgaatgc tgggcgaaac ccggcactac ggtattcccg
    15301 ctacgctgcg gggcatggtg aacaacgacc tgaaggccac ggctgacgcc gtgttgtccc
    15361 tggtgaaaga cggcgcaact gatggtgttc agatcgaccc gacgctgttt tcacagtacg
    15421 gcattcgcag cgttccggcc ctggtggtgt tctgcagcca ggggcacgac atcatccggg
    15481 gaaatctgcg cgtcaggcag gcgctggaga aagtggccgc cacaggagac tgccggcagg
    15541 tggctcaggg actgctggac ggcaccgggg ataagtcgca atgagtttac ctgcaggcta
    15601 ttaccggatt gacccggaca tccgtgcact ggtggccgcg atgaacgttc acggatttcg
    15661 tacatatgcg tcctgtcagg ggcatggttt ccctgttacg aaactgccgc cttatattgc
    15721 ctttgtctgt ccggtaaaaa aggcggctct tcttgagcag cggctgcggc aggatgcgga
    15781 gtccatgatg ccccggttgc tgtggggctg gtccgtcggg gcttctttta acagtgacct
    15841 tcagctctgt ttccggcttc agcctgaagg ccctcatcac tggtatcacc ggtactgtcg
    15901 tcgctcactg cgtgcagact tcaggacgct ggtccgctta ctgaatccgt aaatctgttc
    15961 ccgtaaggag gcattgatgg cgctgacaaa gaagcagcgg gaaaaactgc gtatgaaatt
    16021 cggcggtcgt tgtgcgtact gcggctgtga actgccggaa aaaggctggc atgccgacca
    16081 tgttcaggcg gtgctccgca agtcagagca gtgcatgaag gctgcagaaa agcgcatttt
    16141 caggctgaaa tccaccggtg atgttttcag accggaggcc gactgtccgg aaaacctcgt
    16201 cccggcctgt gcgccgtgca acctgctgaa aacaacatat tcgctggaga tgttcagaac
    16261 gcaggtgtcc ctgcaggttg aacgggggcg cagaagcagc gtgaatttca ggaccgcaga
    16321 acggttcggg ctgattcagg tggtgaacaa accagtcgtg ttctggtttg agcaatatca
    16381 gagggagagt gaggtatgaa acgtcttctg ccattgttgc tggggctggc agtcagtgtg
    16441 gcacaggctg acagcaacag tgattaccgg gcgggttctg attttgcccg tcagattcag
    16501 gggcagggta caggcagtat tcagggcttt aagccacagg agagtatccc cggctataac
    16561 gcgaatccgg acgagacaaa atattacggt ggcgtgactg ccggcgggga tggcggcctg
    16621 aaaaatgacg gcaccacgga atgggcgacc ggtgaaaccg gaaaaaccat tacagagtca
    16681 ttcatgaaca agccgaaaga cattctttca ccggatgcac cgtttatcca gaccggcagg
    16741 gatgtggtga accgggctga cagcattgtg ggaaacaccg ggcagcagtg cagtgcgcag
    16801 gagattagcc ggagtgaata cacgaattac acctgtgagc gggacacgat ggtggaggag
    16861 tactgtaccc ggactgcggc catgacaggt cattatgaga atacgacaga aataaaacag
    16921 attgtgattg aatcttccgc gattcagttc actgagaata acaattcttt ttctggtgaa
    16981 tttaaggtca gtacccgagg aaaagtgatc gctgccagcg cggtttactc ctggaataag
    17041 aggctggcct tcagcaatcc tagctggttt atgaaagtga tcacgccatt tggcttaatc
    17101 tcaatgaatg attctaaggg gagcgttacg ctgagttccg ggctggatat aacagatggg
    17161 cagtcattgc tgttttccat aacgaacaga cacaacaaac ctacagtatc ttccatctgg
    17221 tatagctcaa atatgaggta ccgtttcaca ataacgctga cgatacaggt tccatcccga
    17281 aaatgggtac ctgacgttat atggacggaa agctgcccgt tcagcaagga gctggggaaa
    17341 ctgacaaaaa cagaatgtac ggaggctggc gggaacagga cgctggtgaa agatggtcag
    17401 tcatacagca tgtaccagag ctgctgggca taccgggaca cgtatgtcac acagtcagca
    17461 gacaagggaa cctgtcagac ctacaccgac aatccagcct gtaccctggt gtcacaccag
    17521 tgcgcctttt actccgaaga aggtgcctgt ctgcatgaat atgccacgta ctcctgtgag
    17581 tccagaacat ccgggaaagt gatggtctgt gggggagacg tcttctgtct cgatggcgaa
    17641 tgcgataagg ctcagagcgg gaagagtaat gattttgccg aagctgtttc tcagctggct
    17701 gcgcttgccg ccgcaggtaa ggatgttgcc gccctgaatg gtgtggatgt cagggctttc
    17761 acgggtgagg cgaaattctg ccgtaaggcc gcagccggct tcagtaactg ctgtaaggat
    17821 ggcggctggg gtcaggatgt ggggctggcg aagtgcaaca gtgaagaaaa ggcgctggga
    17881 aaggcaaagg acaataaact gacggtgagt gtgggcgagt tctgttcgaa aaaggtactc
    17941 ggtgtctgcc tgcagaaaaa gcgcagttac tgccagtttg attcgaagct ggcgcagata
    18001 gtccagcagc aggggcgtaa cgggcagttg cgtatcgggt ttggcagtgc gaagcatcct
    18061 gactgtcggg ggattacggt tgatgagctg cagaaaattc agttcgacag actggatttc
    18121 actaacttct acgaagacct gatgaataac cagaaaatcc cggacagtgg tgttctgacg
    18181 cagaaagtga aagagcagat tgctgaccag ctgaaacagg caggacagta aggtatggaa
    18241 aataaccggg gaaaatgatg aaagtcatat tcacgtcgaa ccgttttatt gattttctta
    18301 tccggctttt gattacagcg attgtcattt ccccggtaat tatctggtcg tgggatacgg
    18361 taaaagaaac cactgcaggt gagatgcttg ctgcttgttt tgttattttt tactccggtg
    18421 tgctcctgtt tattctgtat ttttgttttt ctgccctgac tgacctgctg aaaaccgatg
    18481 aaagaaaaaa tgatgaaagg aatgaagatg aataaagcat tactgccact gttactctgc
    18541 tgctttattt ttccggcgtc agggaaagat gcaggctggc agtggtataa cgagaaaata
    18601 aatccgaagg aaaaagaaaa taaaccagta cctgcagccc cccgtcagga gccggacatt
    18661 atgcagaaac tggccgcact gcagacggca acaaagcggg cgctgtacga agctattctg
    18721 tatcccggcg tggataattt tgtgaaatat ttccggctgc agaattactg gactcagcag
    18781 gccgggcttt tcaccatgag tgctaaaaag gccatgctgg cacatcctga actggattat
    18841 aacctgcagt acagccatta caacggcacg gtacggaacc agctggcggc agaccaggcg
    18901 cagcagcgac aggccattgc gaaactggct gaacactacg gcatcatgtt tttttaccgg
    18961 gggcaggacc ccatcgacgg gcagctggcg caggtcatta atggcttccg ggatacgtat
    19021 ggtctgagtg ttattcccgt ttccgtggat ggcgtgatta atccgctgtt gccggattcc
    19081 cggactgacc aggggcaggc gcagcgcctc ggcgtgaaat atttcccggc catgatgctg
    19141 gttgacccga aacagggcag tgttcgcccg ttatcatacg gttttatttc gcaggacgac
    19201 ctggcaaaac agttcctgaa cgtttctgaa gactttaaac cgaattttta acgaggtcgt
    19261 ttaaatgagt tttatttctt tatggccttt attgctggcc caggtgacag tgatgtcact
    19321 gacagtgatt ttccttgctg ctgacaggcg ttttttttca tgggactgtt ttgttgtttg
    19381 caccctgtcc atgatgatgg cagaaggtat tgttattatg aaatatgaac tgtggcatat
    19441 ccctcctgtc ctgctgggtc tggcaggagg gttgtcatgg tcgctgctgc tactccatac
    19501 aggatatttt tcagacatat cagaaggcgt gcataagcgt ttgtggctcg ctgttgccac
    19561 agtgttttct ggtgcgggaa tgattatatt ctactggttg ctccatgcat cagaaaatat
    19621 aataaaataa cgaatagtta atgcgagaat aagacacatc cagagaataa atattataag
    19681 tgcaagtttt gcttttctta ttcgtaacgc ggacaagtaa tttatcacag agaggaacat
    19741 ccagagttca atgcatatta ttgttaatat agaaacagag agtatgataa ttgatggaca
    19801 gttcttttgt accatgtagt ccggtgaaat gtttatcagt aagacaatgg caacattgcc
    19861 taacattgtt tcccggataa tgtctctgat agcttccagt gtttttctgt caaaacataa
    19921 actcatagta ttaaatccgt gataattaac ataactgtgt ctgattgatg aaatcagcaa
    19981 tatactgaat aataatggtg ttttaaatat gagaaaatat aaattctctt taccccggct
    20041 tgatattacg ggtatgtggg tattttccct gggtgtctgg tttcatatcg tcgcccgcct
    20101 tgttcacagc aaacccggga tggctttttt tctggcggag ctgattgcgg ccatccttgt
    20161 gctgttcggc gcataccagg tgctggatgc atggattgcc cgtgtcagcc gggaagagcg
    20221 tgaggcactg gaagccaggc aacaggccat gatggaaggg cagcaggagg gcggacatgt
    20281 ctctcactaa attactgccg gtcattttgt tgatgatggc cacaggtgtg caggcctcca
    20341 cccgggatga aattgaacga ctctggaatc cgcagggtat ggccgcgcag ccagcacaac
    20401 cggcagcagg cacgtcagcc aggacagaaa agccggctcc ccgctggttc cgcctcagca
    20461 acggcaggca ggttaacctg gccgactgga aggtggtgct gtttatgcag gggcattgcc
    20521 cttactgtca ccagtttgac ccggtactga aactgctggc acagcagtac ggcttttccg
    20581 tttttcccta caccctggat ggtcagggtg atacggcctt tccggaggca ttaccggtgc
    20641 caccagacgt gatgcagacc ttcttcccga atatcccggt ggccacaccg accaccttcc
    20701 tggtcaacgt caacacgctt gaggcattac cgcttttaca gggggcaacg gatgccgccg
    20761 gttttatggc gcggatggat accgttcttc agatgtacgg aggggaaaaa ggtgcgaaat
    20821 aagcaggtgg tgttactcat tgccggaata tccggcatcg cgacgggaat aatcgtcagc
    20881 ctgaatatcc cctttatccg tcaggggctt ttttatcccg ccagccctgt ggaaattgtt
    20941 gtttcgctgt gtcttacctt ttctgtttct gttgtgtttt ttgtgggggc aattgtggga
    21001 tggatttcgg tgtctgaaat atattacagc cgcatgaccg gtctgaatga atcatcagaa
    21061 atatcagagg aaacttatga tgacagaaaa caaaaagaat caggaattca aaatcagaaa
    21121 aattaaacgg aatattgagc gcagttgtga taacgccata aaatacttcg agctttttgt
    21181 ggttttcttt gttgccggat tgcttttatg gcacggtatg catgcttttt tcagcgccgg
    21241 tattgattcg tggaaagctg acccggaatt aaataatttg atttatatgt ggaacatcct
    21301 gatgtatgcc attccgtata cgttatatgc gcttgcagcc gggtttcttg ttacatcttt
    21361 tctgtcccca atgtacgaat taatattcgg aaatatcatg atattccagc tgaaacgccg
    21421 tatgcgccgg gagaacacat taagggaggg cagcaataat gcttcgcatt aaacctcttt
    21481 ttattctttg cgccgcctta ctgacggcca tacccacagc gtcagcggat gtgaacagcg
    21541 acatgaatca gttctttaac aagctgggct tcgcctccaa taccacacag ccgggcgtct
    21601 ggcaggggca ggccgccggg tatgcctatg gtggctccct gtatgcccgc acccaggtca
    21661 aaaatatcca gctgatttcc atgacgctgc cggatatcaa tgccggatgt ggcggcatcg
    21721 acgcctatct cggctcgttc agttttatta atggcgatca gttgcagcgt tttgttaagc
    21781 agattatgag caatgctgcc ggttactttt ttgaccttgc cctgcagaca acggtgccgg
    21841 aaataaaaac cgcaaaagac ttcctgcaga aaatggcaag tgacattaac agtatgaacc
    21901 tcagttcctg tcaggtggca caggggcttg tgggggggct gttccctcag acgcaggaat
    21961 ctcagcagaa agtttgtcag gacattggca gtgaaagcaa tatcttcgcc gactgggccg
    22021 catctcgtca ggggtgcacg gtcggagggc agtcctcatc agttcgtgac aaagccagcg
    22081 acaaggacaa ggagcgggtg ctgaaaaacc agaatatcat atgggaggcg ctgaaaaaaa
    22141 acagaatgtt tgacggcaac aaagagttga aagaatttat catgacgctc accggtacgc
    22201 tgatttttgg ggaagacagc gagattaccc ccctgccggc gcgtaccact gaccgcgata
    22261 tcctccgggc gattatggag gggggaacgg caaaaattta ccactgcaac gattcggaca
    22321 agtgtctgaa ggtggtggcg gatgcaaatg tgaccatcgc ccgggataaa gccctgaaat
    22381 cgcagattac aaaactgctc accagtattc agaataaagc agtgagtgac actccgcttg
    22441 acgcccggga acaggggttt atttccagca ccaccattcc cgtttttaaa tatctgattg
    22501 acccgcagat gctcggtgtg tccaacagcg tcgtttacca gctgacggac tatatcggct
    22561 acgacatcat gctccagtac attcaggaac tgcttcagca ggcccgggcc atgattgcca
    22621 ccgggaacta tccgcaggct gtgatggaca atgttctgga gaatctgaat caggcacagc
    22681 aacagattgc cattttccag agtcaggtac aggtccagca ggatgcgctg ctggttgtcg
    22741 aacgtcagat gagctacatg cgtcagcagc tttccgcccg tatgctcagc cgttaccaga
    22801 acaactatca cttcggaggg aattcactgt gaatgaagtt tatgtgattg ccggtggtga
    22861 gtggttgcgt aacaacctga atgccattgc agcatttatg agtacccgga cctgggattc
    22921 cattgaaaaa attgcgctcg ccctgtctgt tgttgctgtg gcttttatgt gggtacagcg
    22981 gcacaacgtg atggatttac tgggctgggt ggcggtgttt gtgcttatca gcctgctggt
    23041 caatgtccgc acatcggtgc agattattga taacagtgac ctggtcaaag tccatcgggt
    23101 ggataatgtg ccggtcggtc tggcgatgcc actttcactg acgacacgta tcgggcatgc
    23161 gatggtggtc agctacgaga tgatattcac ccaaccggac agcgtcacct acagcaagac
    23221 aggcatgctg ttcggggcga atctgattgt gaaaagcacc gatttcctgt cccggaatcc
    23281 ggaaatcatc aatctgttcc aggactacgt ccagaactgc gtgctgggtg atatttacct
    23341 gaatcataaa tacacgctgg aagatctgat ggcctccgct gacccctaca cgctgatttt
    23401 ttcccgaccc agcccgctac ggggcgttta tgacaacaat aataatttca taacctgtaa
    23461 ggatgcgtcg gtcacgctga aagacaggct gaatctcgat acaaagacgg gaggcaagac
    23521 ctggcattat tatgtgcagc agatatttgg cggcagaccg gacccggacc tgttattcag
    23581 acaactggtc agtgacagtt acagttattt ctacggctcc agtcagtctg ccagccagat
    23641 tatgcgccag aacgtcacaa taaatgccct gaaagagggt atcaccagta atgccgcccg
    23701 taatggtgac accgccagcc tggtgaatct ggccaccacg tcatcgatgg agaagcaacg
    23761 tctggcgcat gtctctatcg gtcatgtgac aatgcgaaac ctgccaatgg tacagactat
    23821 cctgacggga attgccattg gtattttccc gcttctggta ctggcagctg tcttcaacaa
    23881 actgacactg tccgtcctga aaggctatgt gtttgccctg atgtggctgc agacctggcc
    23941 gttgttgtat gccattctga acagtgccat gacattctat gcgaagatga atggtgcgcc
    24001 ggtcgtgctg tctgaactct ctcagataca gctgaaatac tctgacctgg cctctactgc
    24061 cgggtatctt tcagccatga tccccccgtt gtcatggatg atggtaaaag gccttggcgc
    24121 aggtttttcc agcgtgtaca gccacttcgc ctcttcctct atcagtccga cggccagtgc
    24181 agcaggcagt gtggttgacg gtaattactc ctacggcaac atgcagacgg aaaacgtgaa
    24241 cggcttcagc tggagcacca acagcaccac gtcgtttggt cagatgatgt accagaccgg
    24301 cagtggcgca acagccacac agacccgtga cggtaatatg gtgatggatg caagcggagc
    24361 gatgtcccgg ttaccggttg gtatcaatgc aacgcgtcag attgcagcag cgcaacagga
    24421 aatggcccgg gaggcgtcga acagagcaga aagtgccctg catggtttca gcagcagtat
    24481 tgccagtgcc tggaacacgc tcagtcagtt tggttctaac cgggggagca gtgattctgt
    24541 cacaggtggc gctgacagca ccatgagtgc acaggattcc atgatggcca gccgtatgcg
    24601 cagtgcagtg gaaagctatg cgaaggcgca taatatcagt aatgagcagg cgactcagga
    24661 actggcatca acaagtacca ggctatctgg tggaatgtat gctgatgctc atgctgaatg
    24721 gggggttaaa cctaaaattc ttggtgttgg tggtggggta ggcatcagag gtggatttaa
    24781 ggggagcctg gatgcaacgg atgatgactc tcattcagca agtagtggca gcagggctac
    24841 ccatgatgct cgccatgata tcgatgctaa agccagtaag gactttaaag aggccagcga
    24901 ttactttacc agtcgcaaag tcagtgaatc cggcagccat actgacaata atgccgattc
    24961 ccgtgtggac cagctgtctg cagccctgaa ctcagcaaaa cagagttacg accagtacac
    25021 gacgaatatg acccgcagcc atgaatatgc tgaaatggca tcccgtactg aaagcatgag
    25081 tgggcagatg agtgaagact tgtcgcaaca gtttgcacag tatgtaatga aacacgcgcc
    25141 acaggatgca gaagctattc tgacaaacac cagttcaccg gagattgcag aacgtcgtcg
    25201 ggcgatggcc tggtcttttg tgcaggaaca ggtacagcct ggtgttgata atgcctggcg
    25261 ggaatcccgt ggtgatattg gtaaaggaat ggagagcgta ccttcgggtg gtggcagtca
    25321 ggatattatt gctgaccatc agggacatca ggccattatt gagcaaagaa cgcaggacag
    25381 taatatccgt aatgatgtaa aacatcaggt tgataatatg gtcacagaat ataaagggaa
    25441 tatcggagat actcagaata gtattcatgg cgaagaaaat attgtagaca gacaatattc
    25501 agagttaaaa aataatcaca aacaggaaga aattcaacaa aacaacagat ataatgaaga
    25561 aaataaacga cagaaattaa tgccaacacc gtctgaagat gcgttaaaac agatggtgga
    25621 tgataagaaa gaacgattga aggggccatt atgatcactc actatgatat aaaaatggag
    25681 atgcaaaaac taaaggaggt actctctgta gagggtgtta atatcccatc attattacaa
    25741 gtcataaagc cgggaactta tgtgttttta tgggttttat tatggccaac ttttctacgt
    25801 ttagtatcgg ttaaaagtga tgtcagggat gttggctttg atatatgtgc tagtggaatg
    25861 atggggttcc tgctttttgt ggctattaca aatggaatga tgttatacct tgcgattcct
    25921 gatagtttca gaaaggattc aaaaataata aattttatgt attctaagag taagacatat
    25981 attttgttgt ttttgattgt tttttctatg gtttcgttta tgcattctat cttatatgtt
    26041 tttgcactaa tgattacatt catattattt ttcttggtgt ataccataga tataaatcgt
    26101 tataatctct cagctatagc ctcagtaata ggactgttca aaaaagagtc tgttagttaa
    26161 aaggatattt tggatgaaaa caaaaaaatt gatgatggtt gcactggtca gttccactct
    26221 ggccctttca gggtgtggtg cgatgagcac tgcaatcaag aagcgtaacc ttgaggtgaa
    26281 gactcagatg agtgagacca tctggcttaa acccgccagc gaacgcacgg tatttctgca
    26341 gatcaaaaac acgtctgata aagacatgag tgggctgcag ggcaaaattg ctgatgctgt
    26401 gaaagcaaaa ggatatcagg tggtgacttc tccggataca gcctactact ggattcaggc
    26461 gaatgtgctg aaggccgata agatggatct gcgggagtct cagggatggc tgaaccgtgg
    26521 ttatgaaggc gcagctgttg gtgcagcgtt aggtgccggt attaccggat ataactcaaa
    26581 ttctgccggt gccacactcg gtgtgggcct tgctgctggt ctggtgggta tggctgcaga
    26641 tgcgatggtg gaagatgtga actataccat gatcacggat gtgcagattg cagagcgtac
    26701 taaggcaacg gtgacaacgg ataatgttgc cgccctgcgt cagggcacat caggtgcgaa
    26761 aattcagacc agtactgaaa cgggtaacca gcataaatac cagacccggg tggtctcaaa
    26821 tgcgaacaag gttaacctga aatttgaaga ggcgaagcct gttctcgaag accagctggc
    26881 caaatcaatc gcaaacattc tctgaacctc gtcaggaggc tggtctgacc ggcctccttg
    26941 ctgacgcctc gcgttgctcg ttgtccaacc ccgggacgta acaaaataaa ttttgtcacc
    27001 tcccggcgtt tccgaaataa atatgaaaac actgagcggt tgtgcaaatt ctttgtgcga
    27061 ccgtgaagtg ttgccggcga agccgggatc ttccggagaa agtcattatg acatcagaca
    27121 atatcgctga atatgttggt gagaataatc aatttactga gaagcctgta actgaatacc
    27181 ccccggtgat acaggataat acagtgacca ctaaagacta tctgtacagc aataatattg
    27241 atactgacct tttagtggtt atcggagggc ttattgttat attgtttctc atgatgctta
    27301 tcatgtactt tattaattct ctcaggcata aggaattaac tgaaatactg gaaaaaatat
    27361 cttttcctga aaaatccggg agcaatttgc ttaaaccaga ggcggtcagc tgtaatttgt
    27421 tgtccctgtg tgtgctggca ccggtattct gtacacccaa aaagctggct gaagtctcca
    27481 gggagatcat cgtggcgagt gcgcaggaaa ttgcatggac gccggggagc agtcgtattg
    27541 atggtcatca gggcgaaaat attacctggt ctctgtcatc gcagtctcct gccgataagt
    27601 atgatctcat caatgaaacg tcccgtgttt ctcctgacag tggccgtttt tatgaaccgg
    27661 acacagttct ggatcgccgt gaacgggtac gcctttttat tcagatccag tttccgcacg
    27721 ggagtgactg gtctgaagta ttgcgcctga ctgacattgt tacagagaag gtctcttccg
    27781 gcagggctgc aggcgggtat caccaccggc atacagggta cacctggtct gtttctgtga
    27841 attaacccca catcatcctt ttctgccctg ttccagattt tcccggagtt tcatcatgag
    27901 ttttaacgca aaggatatga cccagggcgg tcagattgcg tccatgcgta tccgcatgtt
    27961 cagccagatc gccaatatca tgctttactg cctgtttatt tttttctgga tactcgttgg
    28021 tctgatttta tgggtaaaaa taagctggca gacgtttgtg aacggctgta tttactggtg
    28081 gtgtaccacg ctggaaggca tgcgggattt aatcaagtcc cagccagtat atgagattca
    28141 gtattacggc aaaaccttcc ggatgaacgc tgcccaggta ctgcatgata aatatatgat
    28201 ctggtgcggt gagcagctat ggtccgcatt cgttctggcg tctgtcgtgg ccctggttat
    28261 ttgcctgatc accttctttg ttgtctcctg gattctgggg cgtcagggta aacaacagag
    28321 cgaaaatgaa gtcacaggtg gtcgtcagct gacggataac ccgaaagagg tggtccggat
    28381 gctgaaaaaa gacggcaagg actccgatat ccggattggc gacctgccga ttatccggga
    28441 ttccgaaatc cagaacttct gtctgcacgg caccgtcggg gccggtaagt cggaggttat
    28501 ccgtcgtctg gccaactacg cccgtcagcg cggtgatatg gtggtgattt atgaccgttc
    28561 aggggaattt gttaaaagtt actatgatcc ctccatcgat aaagtcctga atccgctgga
    28621 tgcccgctgt gccgcatggg atttgtggaa ggagtgcctg acacagccgg attttgataa
    28681 taccgcaaat actctgatcc ctatgggtac aaaagaggac ccgttctggc agggttcagg
    28741 acgtaccatt tttgcggaag cggcgtacct gatgcgtaat gaccccaacc gcagctacag
    28801 caaactggtg gacacactgc tttccatcaa aattgaaaaa ctgcgtacct tcctgcgtaa
    28861 ttctccggcg gccaacctgg tggaagagaa aattgagaaa acggcgattt ccatccgtgc
    28921 tgtgctgacc aactacgtga aagccatccg ttaccttcag gggattgagc ataacggtga
    28981 gcccttcacc atccgtgact ggatgcgtgg tgtccgggaa gatcagaaaa acggctggct
    29041 gtttatttcg tcgaacgccg acacccatgc ctccctgaag ccggtgatct ccatgtggct
    29101 gtccattgcc attcgtggtc tgctggcaat gggagaaaac cgtaaccgtc gtgtgtggtt
    29161 tttctgtgat gagttaccca cgttacacaa actgccggac ctggtggaga tcctgccgga
    29221 agcccgtaag ttcggtggct gttatgtgtt tggtatccag tcctatgccc agctggaaga
    29281 tatctacggt gagaaagcag cggccacgct gtttgacgtc atgaacaccc gtgctttttt
    29341 ccgttccccg agccataaga ttgcagagtt cgctgccggt gagattggtg agaaagagca
    29401 cctgaaagcc agcgagcagt attcctacgg tgctgacccg gtacgtgacg gggtatcgac
    29461 cggtaaggat atggagcgtc agacgctggt cagttattcc gacattcagt ctctgccgga
    29521 tctgacctgc tatgtcactc tgcccggacc gtatccggca gtaaaactct ctctgaaata
    29581 tcaggcacga ccgaaggtcg ctccggagtt tattccgcgt gacatcaacc cggaaatgga
    29641 gaaccgtctg agtgccgtac ttgctgcaag ggaagcagaa ggtcgccaga tggccagcct
    29701 cttcgaaccg gaggttgcct ccggagaagg cgtgactcag gct
//
LOCUS       DAZGJW010000051        29683 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZGJW010000051 DAZGJW010000000
VERSION     DAZGJW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.051"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(130..777)
                     /gene="gloC"
                     /locus_tag="ACHKIK_002259"
     CDS             complement(130..777)
                     /gene="gloC"
                     /locus_tag="ACHKIK_002259"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO9083422.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(804..1352)
                     /locus_tag="ACHKIK_002260"
     CDS             complement(804..1352)
                     /locus_tag="ACHKIK_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO9083423.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="ACHKIK_002261"
     CDS             complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="ACHKIK_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO9083424.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="ACHKIK_002262"
     CDS             complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="ACHKIK_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO9083425.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLANYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQITEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="ACHKIK_002263"
     CDS             complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="ACHKIK_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO9083426.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="ACHKIK_002264"
     CDS             complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="ACHKIK_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO9083427.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="ACHKIK_002265"
     CDS             complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="ACHKIK_002265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO9083428.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            11026..11805
                     /gene="elyC"
                     /locus_tag="ACHKIK_002266"
     CDS             11026..11805
                     /gene="elyC"
                     /locus_tag="ACHKIK_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO9083429.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGDGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(11782..12675)
                     /locus_tag="ACHKIK_002267"
     CDS             complement(11782..12675)
                     /locus_tag="ACHKIK_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO9083430.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYLQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="ACHKIK_002268"
     CDS             complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="ACHKIK_002268"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO9083431.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="ACHKIK_002269"
     CDS             complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="ACHKIK_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO9083432.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(13806..15038)
                     /gene="ycaQ"
                     /locus_tag="ACHKIK_002270"
     CDS             complement(13806..15038)
                     /gene="ycaQ"
                     /locus_tag="ACHKIK_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO9083433.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="ACHKIK_002271"
     CDS             complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="ACHKIK_002271"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO9083434.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="ACHKIK_002272"
     CDS             complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="ACHKIK_002272"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO9083435.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(17843..20107)
                     /locus_tag="ACHKIK_002273"
     CDS             complement(17843..20107)
                     /locus_tag="ACHKIK_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO9083436.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVYLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="ACHKIK_002274"
     CDS             complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="ACHKIK_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO9083437.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="ACHKIK_002275"
     CDS             complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="ACHKIK_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO9083438.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="ACHKIK_002276"
     CDS             complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="ACHKIK_002276"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO9083439.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="ACHKIK_002277"
     CDS             complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="ACHKIK_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO9083440.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="ACHKIK_002278"
     CDS             complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="ACHKIK_002278"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO9083441.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(25685..26773)
                     /gene="serC"
                     /locus_tag="ACHKIK_002279"
     CDS             complement(25685..26773)
                     /gene="serC"
                     /locus_tag="ACHKIK_002279"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO9083442.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(26972..27664)
                     /locus_tag="ACHKIK_002280"
     CDS             complement(26972..27664)
                     /locus_tag="ACHKIK_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO9083443.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            27794..29554
                     /gene="ycaO"
                     /locus_tag="ACHKIK_002281"
     CDS             27794..29554
                     /gene="ycaO"
                     /locus_tag="ACHKIK_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO9083444.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         7215 a         8065 c         7198 g         7205 t
ORIGIN      
        1 tcccctgcaa tattttgaat ttgcacgatt ttgtaggccg gataaggcgc tcgtgccgca
       61 tccggcataa acaaagcgca ctttgccaga aatccaggcc cgcattaaaa gcgggctttt
      121 ttatgtgcct taccagacgg gcatttcgtc ttgcaggaag ggattatgca ggcgttcata
      181 accaagtgtg gataatggtc cgtgacccgg aataaatgtc acgtcatccc ccagtggcag
      241 caatttatct ttgattgaag aaatcagttg attatgatcg ccacgcggga agtcactgcg
      301 ccctactccg cctttgaaaa taacatcgcc agaaatcagc agctttgccc gatcatcaaa
      361 aaacacgaca tgacccggcg tatgcccagg gcaatgtaac acctgtaaag tcacattccc
      421 tatgctgatg gtatcgcctt cgttcagcca acgatctggc gtcagcggct ggcactcttc
      481 cagaccaaac atacgacttt gcgcaggcaa gccttgcagc cagaactcat cttctttttc
      541 cgggccaaaa accggcacgc cgtaatgttg cgccagttcc gccgctgcgc caacgtggtc
      601 cagatgacca tgcgtcagca ggatctgcat cagtgtcagg ccgctgtcat caacttcctg
      661 tttgattttt tccgcatcgc cgccaggatc gaccagtgcg gccagacggg tttgttcaca
      721 ccagattaat gaacagttct gggagaatgc ggtgaccgga ataatacgat agttcatact
      781 gcccctgttt cgttaagcaa ttactaccag tgccgtgctg gcccggtatc aatatgcaca
      841 aagttactac gtggataata tcctacacca cctgcgcgca tagataacgc ggctttgcga
      901 atattgctta acgcgatacc ttcaatatgg aaatccatcg cctggccttt agtgtgatag
      961 cttttcttcg ctactccacg gctgcgggcg cgtagttcat tgttggtatc aatagaacga
     1021 taaccggaaa tgagttgcac cggtttgcgc gtgcctaaca acccttgcag gcgatacaac
     1081 tggtcgaata atcctgggtc gatggacttt attttgttcg cgcggtaatc gcggaaaaaa
     1141 tggttaagtt ttgccaattc ttcctgaata tagcctctgc catcgaaaaa ctccgctttg
     1201 attgactctc cggtatgaag attattgagt gtcaaaatgc gcgggcgtgg ggtagagagt
     1261 gttgcaaacg caggggtcgg caggatggcg gcaccgagtg caacgccacc aagcgccagc
     1321 aatttgcggc gattagcgtc gaatttgtcc atgataatca agtctacagg tcaatgttat
     1381 cgtttatatg cacttctggc gcacaagagg caccttaacc gcccgtaaag cagacgtcaa
     1441 gatgactcaa ccccagcagt gacgggggct gcagagaatc gcccccaatc atgctaatta
     1501 ttacgacaac tgatttcccc gaactacttc atttacctga ttaattgttc cgctttcgat
     1561 acgatttgcg agctggatcg cgcaggcaga tcataattgt aaatatctgt acgatactgg
     1621 gtacgaccat ctgcaccaac aaaggccgtc aggtagtaga gattcaccgg aatcgactgc
     1681 cgaatattga cgtaacgtgt atcaccctgc ttcagcgcat cagaaatacg tttgtcattc
     1741 cagcctgcat cctgcaacag catattcgcc agatcggaag ctttattcac tcgtacacag
     1801 cctgagctca atgcgcgtgt atcacgcttg aacagattgt ggttcggcgt gtcatgcaaa
     1861 taaatggcct ctgaactcgg catattgaat ttatagcgcc ccagcgagtt ccgtgggcct
     1921 ggagcctgct ggaagcggaa cggtaaattc gaggccgtga ttgtagacca gtcaacctgc
     1981 catgggtcaa tcgcttctct gctgttccag ccgcgcatca ccgtatagcc atggctttcg
     2041 agatatcccg gatcgttgcg cacttttggc agaatatctt tgcgtgccag agttggaggt
     2101 acgttccacg gcgggtttac cactacgttg ttaagggcac tgctcatcat cggcgttttg
     2161 cgatcggggc gaccgacaat gactcgcgaa tccagcacct gattgccgtt ctgatagtag
     2221 accagcgaat aggccggaat gttaaccatg atcccggtag aaagctctgt tggcagcaag
     2281 cgcaatcgct ggatgttgag agccaacacg ccagcacgct gggcgggcgt tacgtttaac
     2341 cagtcacgtg ttgccgggcc aatagcacca tctgccccca atccttgcca tgcctgaaaa
     2401 cgtttaacgg cttccaccag ttcattatcg taggcggcac gaacggcagg cgcaggttta
     2461 ctacgagacg tcgtttgctt gtccatcggc ttagtttctg ctgtttcaac agtaacagcg
     2521 gatgggctga ctaccgcgtc agttggcgtg tcatcgccag gtagagtaat tttcggcccc
     2581 ccgtccaaca tgcctgtgcg ttgcaatatt tcgcgcaacg ccggtacgtc gttactccac
     2641 tgccctgggc gcaacgttgc tttgccggtc agttggggcc acggtttggt gtcactgagt
     2701 aaggccagta acgattcatg catcgccgca tattgcggat gctgcggtgc cagtcctgca
     2761 acaaacgtag gcaattgacc tttatccagc gccagctgcc attggttaat caccgagagc
     2821 ggcggcgttg caagtgcata aggtttactg ctatatagcc agcgagtgcc tttgaccgga
     2881 atatttgcaa tgaaatggag atagcccatc atcgcatcag agagcactac gtcgcgtgcc
     2941 atcccgttaa caccaggatc ggtcagtaac tctacccatt tattaaactg cggctggaaa
     3001 ccggcaatcg ccacctctgc cagctgttgc tggaaggctt taacagcatc acggttttcc
     3061 cacatgggtt gcatatcgcg tgcggcatac aacagttgaa gctggttaag ataaaccggc
     3121 ttgtaacctg cgggtaattg agattcgatt tgcgtgcggg ctttttctgc cgcaccttca
     3181 ggcaatggct ggatccccgc cattattgcc gtggcttgcg cctgcggcag tgcctcgccc
     3241 tgttcactga cagccaccgg gctgtcgcca gggattactt caggctcatc ggcctgcgca
     3301 ttgaacagtg gagcgaatgt tacggccagg cacaaactga ttgccgacag ccgacgacca
     3361 cacatcatat taagcaacat cccttgcccc ctgtttttat catactctga atagccaggc
     3421 tgttatttta cgacttgcct gtacaacgcc aatactcacg aaagtatgac gagcaagcct
     3481 gttactcatc aggcttatct tcagtatata aacgccgata cgtttttaca taacctaatg
     3541 taaagaagtt taaagaaaag cgcgccgtaa tgtgccactt ttttagtgcg gaaacggagt
     3601 tttcggaaaa agaaaaggcg gcattgctgc cgccttaatt ttaactcgcc tgagaaggcg
     3661 cttcgtcact tcctggaagc gtttcaggga gttgcggcgc aaatcctcgc aggccgacga
     3721 cgtgaacgtg ttcggtattc tggaagactt tacgcaccag tttataggtg gtgcctttct
     3781 ccgggctgat attttccggc gctgcgatga tgagctgcat ttgcaaacgc tcacacaatt
     3841 caaacagcgt ggcgatagaa cgagcatcca gtcgcgctgc ttcatcgagg aacagcaggc
     3901 ggcaaggaga gatatcttta ccgcgcaggc ggcgagattc atcttcccag ctttgtacca
     3961 ccatcaccag aatcgacata ccggtaccaa tcgcctcacc ggtcgacaat gcaccagact
     4021 ctgcgcgcaa ccaaccatcg gaaccacggt taacctcaac ttccatttcc agatagttgc
     4081 ggtaatccag cagttcttcg ccaatggtct gcggcgtgcg ctgtcccata tcaatctgcg
     4141 gattaagacg ctgatacaat ttcgccaacg cttccgagaa ggtcagacgg ttgctgttaa
     4201 acagatcctg atgctgctcg tgctgttcag agagcacatc cagcagcatg gcgtgcgttt
     4261 cacgcacgtt cacgttgagg cgcacgctgt tcacctgacc aaacgatacg ttctgcaacc
     4321 cctggttgag catacggata cggttctgct cgcgctgaat ggttttgcga atgatgttcg
     4381 ccacgctgcg ggaactgatc gccagtttct gttcacggga ggttaattct tcggtcagac
     4441 ggctaagttc gatctccatc tgttcgatag cttccaccgg atcatcggta cgaataatat
     4501 cctgacgaat acgttcacgc agatgctgat aaaccgccac gaagaactga attttacgct
     4561 ccggacgttt cggatcttcc gacatgcgca gcacgtcgcg cagatgttcg ttatccgcca
     4621 ccgccagacg cagcgcaccc aatgccttat ccgacataga gcgcaaatca tcagcggaga
     4681 gataagccag ctcacgacgg tgtaagcggc gctcaacgcc gttatctttc accatgcgca
     4741 tcaccgcaca ccagcccgct ttggcggtca ctacctgctc gcgcatctca aagtaatccc
     4801 gctccagctt gcgcagtttg cgggtcaggt tgtccatctc cgcttcgcag aaggtaagcg
     4861 ctttttccag ttgattgcgg cgtgaacggt tattgctcag ttgcgcatgc agctcgtcac
     4921 ggcgaatacg cgcccgctct tctgccccgc tatcagcacg cacgccgata tcctgcaatt
     4981 cacgttgcag atcgttgagt agctcttttt tggtgtcgta agaacttttc agcgaagcca
     5041 gcacctggtt gtactgactc aactgcgctg cgtgaccacg caacgcttca cgagcgcgag
     5101 tacgctccgc ttccgcctgt tccagacgtt cacgcagctt ttcgttgaga tcgctattac
     5161 cgctcagcat ttctgccgag tcagaatagc taaagtgcgc acgacgctgc accacttccg
     5221 tcagggcaaa cgcctgctga cgggcatcgc gctgcatctg ctgagagtac gcgtaatctt
     5281 cttttagctg ttcgaactgt tccgggtcgc tctgcaatac cgaaacgatc ggttccagtt
     5341 tcgccagttg attgccaaac tgctgaacaa aacgcgcggc ttcctgggct tcatccagac
     5401 gttcgcggat ttcatcgacg cgatccgcca ggctgtcatc agccaacagg ttgagacgcg
     5461 gcagaatgcg gttcagcgcc gtaacgcctt ctttcgcctg ctcaaactga atacgctgct
     5521 gctggttatc attttcatga ttactcaacg cccgctccag ttcgacgcga cggctgttca
     5581 gttgacggat ttctgcttcc gggtcagact caaacgcaac cgccagatga ctgccgataa
     5641 agcggctgaa cgcctgatgc agacgctgag ttttctgtac atcaaaggag agcgtggcga
     5701 agcgttcgga aagcacttca cgctcggcat ggaggctttc aatacggctt tcacgcgcag
     5761 cacgaccaaa cagcggcact tccgggaaac gtgaataacg ccactgacga tcggcgattt
     5821 tcaccactac cgctttttcc agctcatcaa cgctgaacac gctgtcatcg aatgactgcg
     5881 gatctccttc gatcagatag agatcttccg ggcaatcggt caagccttcc aggtgttcag
     5941 ttatctgtga cagatctggc accacgatgg cgtggcgtga cgggccatac agcgctgaga
     6001 agtacggcgc atcttccagg ctaacgtcgt cataaatttc tgacagcagc acaccaccaa
     6061 aacgctccgc cagcgcgttc agacgctgat cttcagagcc gccaggctgg cttaaacgtt
     6121 cgatctcttc atcgacggcg tttttgcgcg cgcccacttc atcgcgttca acaatcgcct
     6181 ctcgctcacg ctccagcaac tgttgcagat attcggtgac gtcctggctg gaggtaaact
     6241 cttcgccgca ctgttcgctc aactggttga gactgttttg cgctgccagc caaaccggcg
     6301 cacgctgcat caaactctga atgcgagact gcagctgttc ctgttcctgg cgcagtgcca
     6361 tgcgctcttc acgggcgtta gacacgctat cggaaagaga ggcaatgcgc gcttccagct
     6421 cctgatgcag agcttccagc tcgtcaatgt caaaattctt gccctgacgt ttgcagaaat
     6481 ctgccagcag acgctcagct tcttgctgtt cgcgcagacg ctgttccagt tcgcttaagc
     6541 gcatccgcaa cggctgaacc tgctctgcca ggtgacgctg atcgacccct tcgcgcaata
     6601 gttcgcgagc gacatcccac gcctcgttac gcgccagcgg gccgttgatt gccaccacca
     6661 gctgataagc ctgctcaaac tggctgtgcg cggtttgcgc catgctcatt ttctgctcaa
     6721 gagagagcat tttttcagtc gcttccagct ctttcgcctg gaaggtttcc agccattcgg
     6781 cggcgctgtc ggcggttaag tccggcagat ggcacagttc tttggcgcga ttaagcgcag
     6841 caatcgcctg gttatactgg atcgcgcgcg tttgctggac gtccagcgcc tgctggtagt
     6901 tagcaagctg gcttttcagc tcgtccactt ccagttcggc agcttccgcg cgagcctcgt
     6961 tctcttcctg gcgttcgatg gcttctgcca ccacttcgtt ttgctcttcc agacggatct
     7021 gcagctcatc gagatccgct tcgtagcgtt cgatcttctc ctgctgacgc agtgcggttt
     7081 gcaccaggtt caggtgatcg ctggccgcct gataatccgc ttccagatcg ccttcggcac
     7141 cgttgtgctc tgccagctca cgcgccatat cgacgtgttt gtactgctca gccgccagtt
     7201 gctgacgcga agtatgtaac tcgcgacgaa actccagggc tttgtcgaga tggacacggc
     7261 gctcgttggc gtgacgcatg tagtccgccg ccacgtagtt ggtagcttcg ctgatcagat
     7321 gcttaaacag gtcacggtcc gactgggtga cacgaatcgc ttccagcgtc atgcggtttt
     7381 cacgcagcgc cgcttccatg tcctggaacg ctttacgcac gccgctgttt tccggcaaca
     7441 ggtagtcgcg cagagaacgg gtaatggcac tggagatccc gccatacagc gaagcttcga
     7501 tcagacgata gaatttgcta cggtcagatg cggagcgcag acgacgcgcg atgatgccca
     7561 gatcgaacat cagcgagtgg taatcggtaa tggagttgaa ctgtttaaac tgcacgcctt
     7621 ccatcgcctc gagcttgtct ttcagctcgt taagcggcag cacgcgcgcc tggcgttcgt
     7681 tcagggtttc ggtcaccagc tgtgtcggct gcaccgacat cggcagtccc tgaatggcaa
     7741 atggtttgat atccacttta cgatcgcgtc cggcaacctg ttgcagacgc acaccgacca
     7801 ccacgcgctg gtggcgcgag ttaatggtgt cgagcatcga ataacagaca cccgctttca
     7861 gcttaccgtg cagaccttta tcgcgcgaac cgctggtggc cccggcttcc gtagtgttac
     7921 ggaaatgcag cagggtcagg tcggggatca gcgccgtaac gaacgccgcc atggtggtgg
     7981 atttacccgc cccgttaccg ccagaaagcg tcgtgaccag ctcgtcaagg tcaaaagttc
     8041 gggcaaaaaa gccgttccag ttaatcagcg tcagtgagcg aaatttaccg cgttcaatca
     8101 ttattcttcc tctccgctat ctggctgatt ttcttcggtt tcatcgttga gttgcagatg
     8161 attttcaatc ggcattgctt cgccatcacg aatcaggcga cgctgtgctt cacggggatc
     8221 gtcgccagca cgcacatcgg ctccgaagcg gaacaccgat tcggtaatgc gaaacttgct
     8281 gctgtcgtgg cccataaacc acaccatgcc taaacgacgc agacggttga gcgaagaacg
     8341 tactttctcc tgcaacttct gacggtcaac gtctgaaccg gttgaacggt tgttcaccag
     8401 tttcagcagt tttgcttcat cggccagggt gagcagttcg tcgtacagtt cctgctgggt
     8461 gaaaatcccc tcattcgcca gccgttccgg gctgagatag agataacaga ggattttccc
     8521 gaccatcata tccagttccg acaagacgga acgagggatc agcgtggtgg aacgtgggcg
     8581 taaatagaag aacccttctg gtgcgcgaat aagctcaacg ttataacgcg cgtaaaactc
     8641 ttccagatat tcctgaaaat ccatcaggaa tgcatgatta tccagttcgt cgaggccaat
     8701 atggcggcct gaacgtaagg cgctgtccag cgccggaaat aacggattcg ccagcgcctg
     8761 cgccagctta accggcatca cttgttcaat atttgtcgat gacatgcgcc tgtaccttgg
     8821 ctccgtaatc attaatcggc tgccatttcg ctggcagtcc ggtgaaatct gcttgcgcta
     8881 cgccaagacg taccgcctga tcaataacaa tacgcgcaac gtcaaagtga cgtgcacgcg
     8941 gatactgtga cagatattcg cgtaccacca gaccaagatc cagcggcact tgtctggttt
     9001 tgtacacggc aagttgttct tcgatgatcg ccgccagctg ttcgcggatc tcgttaaact
     9061 cttcgtattc cagatcctca ggaagttccc cagtcacttc ttcatcgcgc agtgccatct
     9121 cttcgtcacg catatccagc agacgatcgg cattggcata agttagcgcc cacggctcat
     9181 caaaataggt ttgtaccgac tgacgtaacc gctgagcaaa gacgcggttt ttatccatat
     9241 cgatcgcggt acgaataaat ttgtgtacgt ggcggtcgta gccaatccac aagtcgatgg
     9301 attgctggcc ccaactgata atacgatcga gtttgctctg cagatcgaac accagacgat
     9361 cgacgaaatg cagatcgtca tgggtcatcg tcgcatcctg aatgcgcaac agattagcct
     9421 gcaatttgtc gcctgccgct tccagcgtat cctgcaattc acgcagcgtc ccggaagttt
     9481 ccgaaagcaa caattcacag ctggaaatcg ccgcccgcca gtctttgttc agcaactggg
     9541 cgatatcgtc cttcacctgc tgctgctgtt cgtccatcag acgttgcgtc aggtcgatac
     9601 tgtcgaaaat ttctgctacc gaatatttca gtggcgcata gacattacgg tgccagtgaa
     9661 attcatcacc gccctcttcg gcggcatctg ctgcgcgttt gagctcaccc gccacaatcg
     9721 acaactgcat agaaagacgc agcgtagaaa actcgcgctg acggatgtag tagtcagtaa
     9781 tgccgatgcc gagcggcgtc agacggtaaa ttgcgttccc ttccgcctgc tcgctggtaa
     9841 agcggttcag cagacgttga cgcaccatgt cgttgatcgc gttattggcg cgcacgccga
     9901 tggtttcgct ggtttgctca aacgcatcac tcacatggcg gaatgcatcc accagctcgc
     9961 cttcactcat ctcaccatcc agacgctcgc cgttcagcgt ggcaaccgcc agcagaaaag
    10021 agagtcggtc taccggcagc gagatggaga agtcattttt tctggcccag gcaaccagtt
    10081 cggggactgt ctgggaaaat tcactcatac tttatccttg ctctgcggtt tgcgcgcggt
    10141 gacatgaata taacgcccca gggtaatata cggttcctga cggcaataac gcgtttctaa
    10201 ttcaagtaat gcttcatagc agtcgcgctg ctggtgcttc tcgcgcagat aatcatgaaa
    10261 cacgcgaacg cctgtcttac ccataatttg ccaaccagct tcttccagcc acagataaac
    10321 ctgcgccggg tcgcgtggat aatctggcga aagcgtccgt tttttctttt tcggcattcc
    10381 cgcctgcacg taatcaaaat tcccggcgac catgttatgc atcaacaaac catgcgcatt
    10441 gtagaacatt aacgacaaca cgccgcctgg acgcaacact gaccacaggg tctgcaatac
    10501 actgcgggga tcggccaccc actcgagcac cgcatggaac aatatcagat caacgggcgt
    10561 ttccaaatgc gaagcaacat cctgagcggc gcaatgtata aattgcatgt tgtcgctcac
    10621 accttttgct tctgccgcct gttttgcgcg gtcgatcatc tgcgcagaaa gatcgcataa
    10681 aatgacctga tgcccacgct cggccatttt gattgcggtc tgcccttctc caccgccagc
    10741 atccagcaca cgcagttttt gcgggcccat ttccgccagc acgcgatcga gatcctgcca
    10801 cagaatagcc tgtcgaagct gccctttggt ggtgccgtaa atgttacggg aaaacttttc
    10861 cgcaatatca tcaaaattgc gatcctgcat tgctttctcc accggttggc taagacgttt
    10921 tctgataaag acgctatttt gtcacagcca cggcgagaat gaacccgtct ggtgtaatat
    10981 cgctggcgat cccttgctat atggttaaaa aaggaaccag aaaggatgct ttttacactg
    11041 aaaaaagtga ttggcaatat gttgctgcca cttccgctga tgttgttaat tattggcgct
    11101 ggcctggcgc tgctgtggtt tagccgtttt caaaaaaccg ggaagatttt tattagcatt
    11161 gggtggctgg cactcttgtt attgagtttg caaccggtag ccgaccgcct gctgcgtccc
    11221 atcgaaagca cctatccgac atggaataat tcgcaaaaag tggactatat cgtggtgctc
    11281 ggagatggtt atacctggaa cccgcagtgg gcaccgagct ctaatttaat caataacagt
    11341 ctgccacgcc tgaatgaagg tattcgctta tggcgcgaaa atccgggatc aaaactgatc
    11401 ttcacgggag gcgtagcaaa aaccaatacg gtgagtacag cggaagtagg tgccagagtt
    11461 gcgcaatcgc tgggcgtgcc gcgcgagcaa attatcaccc tggatttgcc aaaagatacc
    11521 gaagaagaag ctgcggcagt gaaacaggcg attggtgacg cccctttcct gctggtgacc
    11581 tccgcatcac atctgccgcg ggcgatgatc ttttttcagc aggaagggtt aaatccgcta
    11641 ccagccccgg cgaatcagtt ggccatcgac tcgccgctta acccgtggga acgggcaatc
    11701 ccttctccgg tctggctgat gcatagcgat cgtgttggct atgaaacgct tggccgcatc
    11761 tggcagtggc tgaaaggtcc gtcaggcgag ccacggcaag agtgattttg atgccagatc
    11821 gaagttgcgc cgactaaatc gtccggtatt aaccagttgc gcaacttcat cccataacac
    11881 atacaaccaa cgccgccaga tgaacgactc cgccactggc gcgcgttgca ggtaactcca
    11941 gagcaaatct tccgccagag aattatccat tagtcggaac agttcgtatt ctcgcggtgc
    12001 ccaaagcatt agtcccggcc cgaccatcgc cagtaactga tcgctgcgcg aatctttcag
    12061 catgctgcgt aaacagaagt taccgtgaat caacacacaa ttgtcgttaa agccttcaaa
    12121 taatgccggg agacattcgc gagtgcgaaa caggatccgc ttatcctgca tcgtcagacc
    12181 ggtgttattg aactgattga gcgtggtcca tagcacttca acatgttgca ggtaccatga
    12241 gggccagaaa ttttcctgag tattgtcgac cgcgccgacg caaccgcgac tgtcctgacg
    12301 gtgccaggcc agtaaggctt caacgatttg gtctttgagc tgttcccagc gttctggtgt
    12361 tcgggctggt gcctccaccg aaacaccacg catccgctcc agtagcagga catcagggcc
    12421 ggggtgttct tcatgcgtca tcacaccata aatagtcggc atacggacag tcccacttct
    12481 tgccagcatg gtggttttcc atgccagttg tcgggctttt ccgggcgtac taaagcttct
    12541 tgccattaac ggcattgggt ttccctggct gtcatacaaa gcccacaacg ccgtatccgc
    12601 tttttcattg acgcactcaa tacgactgag tttttcgccc agtaaatggc ttaattcggc
    12661 acgcagctgt tccattcaga ttaccctcat aaaagctact gctttcataa tgagcgccaa
    12721 tataaaagat gtcacctgaa tgagatcaat tgttgaaaag aaaaattgag gaaaatcgta
    12781 acggtacgac actcctccca aaattggctg aagtgtcgtg aagtgaaatt agcgcatttc
    12841 agcgcgaacg cgctcaagat cttcaggggt atccacacct gtgccaggaa cttcctgagc
    12901 aacagcaaca tggatttttt cgccgtacca cagaacacga agctgctcta acatttcgat
    12961 gtgttctaac ggacttgcct gccagttgac gtaacgacgg ataaagcctg cacggtagcc
    13021 ataaatacca agatgacgca ggaagttatc gccaacggtt tcaaggcctt ctgcaaaacg
    13081 atcacgatcc caaggaatgg tggcgcgtga gaagtacagt gcataccctt cagcgtcgag
    13141 aaccactttc accgcattcg ggttaaacgc ttcttccgca ttgtggattg gcaccgccag
    13201 agtcgccata cccacctgac gctgagcgag gttatcagca acctgacgaa tgattgtcgc
    13261 agggatcatc ggttcatcac cctgcacatt aacgatcacc gtgtcgtcgc tgaatgcgca
    13321 tttttcgaca acttccgcca gacgttctgt tcctgactga tgatcggcgc gcgtcataca
    13381 tacttcaccg ccagcggctt caacggcgcg ggcaacatcc tcatgatcgg ttgccacgat
    13441 gatgcgctcg gcacctgatt cacgcgcgcg ttcaagaaca tgaacaatca tgggtttgcc
    13501 gttaatatca accaatggtt taccgggcag acgcgtggac gcgtagcgcg cgggaataat
    13561 gaccacaaaa ctcatgattt actctcatca gcagtcagca cgcgggcttc cgtttccagc
    13621 aacactggaa tgccatcgcg gagcgggaaa gccaggttat ccagtttgca aataagctct
    13681 tgtttttcct ggttatacca aagttttccg ttgcaaaccg ggcaggcaat gatttcaagc
    13741 aaacgatgat ccatagttcc tccagatgga tcgggttatg aatgcataaa tcttatcata
    13801 atcatttatg cgacggggtc tatttcccag ccagtgcggc aatcagtaaa gagaccttgc
    13861 gggcagcgtc ctaatgtcac ccgcgttgcc tgctgccagc tcgcgaaatc agtaatcgcc
    13921 tgacgtaacc ctttttgcag cattgtcgtt ggtttaatgc cttcctgtaa ccacagagag
    13981 ataacttcaa ggatgcctgt ctggcgatgc attttggcat ccattcgccc aactaattgc
    14041 ccacgatgta ataatggcag aacaaaatag ccatactggc gtttcggcgc tggggtatag
    14101 cactccagcc ggtagctaaa atcaaaaagc tgctctgcgc gtttgcgatc ccagacaaca
    14161 ggatcaaaag gcgaaagtac cgcgctgtgc gttgcagtga gctttccggc cagcgcacgc
    14221 tcgagtagcg gcagcaaatc agcatgcagc caaagattgc ccaatttttc aacatgcaca
    14281 gcaatgattt gctgctgttc agccctcgct tcgcgccacg ccgccagtgc cggacgtttc
    14341 agccgatagt aatctgccag ccactgttcg cggaatattc ccaggctacg cgcactgtta
    14401 tccagcatga tgatttctgc ttctgtttgc gaaacgagat cgcgctcatc atcccagtca
    14461 ggcatgacac ggtgggttaa atcataaacg cgctggaagt tgcgccgttc aatcaccatc
    14521 acctttccgg cagtaaataa accttccaga tgccgtttat gcggcttcca ttcccaccag
    14581 ccgcttgcac ctttacgagg atgctcaaaa tcggctgaac gtaccggccc cttatcatga
    14641 atatgctgaa ttaactgtgc aatttccgcc tcatgttcct gcatccaggc gtctttgtat
    14701 ttccagccca ttttttcagg tgccagcatg cggtggcgaa taagacgaaa gtcgctacgc
    14761 ggcataaagc aggcttcatg cgcccagtat tccattaatt cgccacgcgc cagagactca
    14821 tccagccact gggcaggata atttcccaga cgactgaaaa gcaccagata tggactacgg
    14881 gcaacaatat tgatggtatc gatttgcagc aaggacatgc gggagatcgt tgccggaata
    14941 tcctccaacg acgcttgacg gcggggtttg tttaacaggc cttgtgcggc aaggtgaaga
    15001 ttacgcgcat cagcaaggga gaggtgcggc agcgacattc atgactccat caatcgaacg
    15061 ctgccgcggc gtaactagtt gccagaagcc agcgaggtta gttgcgtaag cagtttcgct
    15121 ggttcatcac ctgaaagctg tgcgtctaca ggcaaatacc accaattttc ttctgcaaag
    15181 gcccggcatt tcaccgcatc tttttcagtc attaccagcg tttgcccggc gcttaccaac
    15241 gcactgacat ccgcatggtt caaagactga tgatcggcca gcggtacaca tttttccggt
    15301 tgtacgccac acatcttcag cgtggcaaaa aagcgcggcg gatgcccaat ccccgccatc
    15361 gccactacat gttcaagctg agcaacgtca caacgcgtac cggtacgtaa attcaccgcc
    15421 tgacccggca gcagatgcat ggggatttca ccgctgcgag ggacaccgcc gttgacgatt
    15481 accgcatcaa ccgactttaa gcgccccgct cgctcacgca ttggccccgc cggcaaccac
    15541 cagccattgc caaagcgacg cacaccatca ataacgacaa tttccacatc acgcgccaga
    15601 cggtaatgct gtaaaccgtc gtcggttacg atgatctgca catcagggtg ttgcgccaga
    15661 atggctttta ccgcatcaga acgaacggga gaaaccgcaa caggcgcatc agtgcgttga
    15721 taaatcaaca caggttcatc acccgcctgt gctgttgtgg tatctgccga caataacagc
    15781 ggataagatt cagccttacc accatatccc cgcgatacga cccccacgcg aataccgcgc
    15841 tgttgcaact gttccaccag ccagacaacg accggggttt ttccgttgcc gcctgcggtg
    15901 agattaccaa ccacgacaac cggtacgggg gcacgccagg cgcgcttcag ttttagttta
    15961 tagcaaagac ggatcgcgcc acttaccagg ccatacaacc aggagagtgg cagcaatagc
    16021 cgccacaaag gggattcacc agaccagatt ttttcgatca ttggccaaac tgcattttgt
    16081 gaagttgcgc gtaaacgccg cggtgctcaa gcaaatcgtt atgcgtaccg cgttccacaa
    16141 tgacaccatc ctcgacgacc acgatttcgt ctgccttttc aatggtagac aagcggtggg
    16201 caatcaccag agaggtacgg tttttctgca actcatccag tgccgcctga atcgcacgtt
    16261 cggattcggt atccagagcc gaggtagctt catccagaat cagaatcggg ctatcacgca
    16321 gcaaggctcg agcgatagca atacgctgac gctgaccgcc agagagcagc acgccgtttt
    16381 caccaatcac tgtatcgaga ccgttatcca tcttattgat gaagtccatg gcgtaggcca
    16441 tacgcgccgc ttcttcaatt tgctcacggc tgtactgttc agtccgtgcg taagcaatgt
    16501 tgttagcaac cgtatcgtta aacagatgga cattctgcga caccagagca acctggttac
    16561 gtaacgacgc cagggtatac tcgcgcagat cgtgaccatc catcaggatt tcgccttcat
    16621 caatatcgta aaaacgagtg atcaggctgg cgatggttga tttacctgaa ccagagcgtc
    16681 caaccagagc aaccgtcttc cctgccggaa ttttcaggtt gatgttacgc aatgcaggta
    16741 cgtcacgtcc cggataagta aaggtgacat tgcggaattc cacgtcgcca gtcgcacgct
    16801 cgatcacgcg cttaccttcg tctttctcct gctcactgtc cagaatggta aacagcgtct
    16861 gacaagccgc cataccgcgc tggaactggg cgttaacgtt ggtcagtgat ttcagcggac
    16921 gcatcagtgc aatcattgaa gagaaaacaa cggtaatcgt accggcagtc aggctatcca
    16981 tgacacttgg gaagctcgcc gcatacagaa caaacgccag cgccaaagag gcgatcagct
    17041 gaatgatcgg atcagagatg gaagaggctg aaaccatttt catcccctga agacgcattc
    17101 ggttgctgac tttatcaaag cgtttcgttt ccacttcctg accaccgaaa atcaatactt
    17161 ctttgtggcc cttcagcatt tgttcggcgc tggtggtcac ctgccccatg gtgttctgca
    17221 tgtttttact gatgttgcga aaacgcttcg atactacgcg aatcgcaatc gaaacaatcg
    17281 gtgccagcac aatcaaaatg atcgacagtt gccaactgta atagaacatc atgatgaaca
    17341 ggccgatgat tgacgcacct tcacgcacaa ccgttatcag tgcgccggaa gaagaagaag
    17401 caacctgttc ggaatcgtag gtgatacgtg acaacagcgt ccccgttgac tgtttgtcaa
    17461 aaaatgaaac cggcattccc atcatgtgac caaacaggcg gcgacgcatg gtcattacca
    17521 cctttcctga tacccaggag atacagtagc tggagacata gctggtgata ccacgtaaaa
    17581 tcatcagccc gatcaccacc agcggcatcc acaccagcac ggagcgatct gttttaccaa
    17641 agccatcatc aagaagtggc ttaaggagcg ataacatgaa ggtatcgctg gctgcgttga
    17701 ggattaacgc tacgcccgcc acgatcagac ccgctttaaa aggcgcaatg gttggccaca
    17761 gtcggcggaa cgtctgccac gtagagagat ctttgtcgtt atgcattcaa aaaaccagca
    17821 tttgttgaaa tagccgcata ttctacccgt tatccactgg cacgccaaac cactgatgat
    17881 accaacgagg taaaatttgc tctctgaggc tgctaatccg ccagccttgc gctgaaaaat
    17941 tgaccgtcac ttgaccctga tgtggagtat caagccattg atatccttgc tgttgatagc
    18001 gatgcttaac tttattagag ggcaatcgcc atgcgttata gcgcgatgcc gatgcgagtg
    18061 ccacttttcc attcactcgc tgaattaatg gcaacgatga tgaggtatta ctgccatggt
    18121 gaggtacctg aagcaatgtt gtctgcactt gctgccagta acggcttagc atcttttgtt
    18181 cagcggggac ttcaatatca ccggttagaa gaatgctatt cgtcccgtca tcaaccttaa
    18241 ccacacagga atggttattt cctttatcgt tgctagcttg taaaggccag tgcacgctga
    18301 aacgcaatcc ttgccattgc cacgcttcgc cacgcacaca gggctgatga tgttcccagt
    18361 ttaacggact tctgatccat aacatcggcc atgtgtgcaa tattgagtcc agccctcccc
    18421 ggtgatccag atgttcatga ctcagaataa cgccttccgg ttcaagatta tgccagtgga
    18481 gccaggggat aatcagttgt tgcccactat ccccttcggg ccaggccagt cctgtgtcat
    18541 agagaatcgc tttgccgttt ctggctatca ccattgccag cccttgcccg acatcaagca
    18601 tgtacacctg ccactcgtca ggtcgaggtt tttgccacag cggccagcac atcagcaagc
    18661 ctacagccac acacattgct ggcaacgttc gccaggcgtt taatcgccat accaccagta
    18721 agaaccatgg ggaaaatgat agccattgcc aacgttcagc aatgttgatc cacccttctg
    18781 gcaaactctt taacccccag aaaagtaaag ccaaagaccg gtcggcaaga aaccataacc
    18841 cttgctccag gattaacggc ccgcttaaat gcacaaccat cgcggcgagg atcaacggaa
    18901 ccgtgataaa tgtcaccaag ggaattgcta atagatttgc aataaacgag gtcagactaa
    18961 tgccatgaaa tatgacgatt tgcacaggca taagcaggag tgtgattccc agttgcaaat
    19021 gaatgaggga aacaactgca cgcaataccg gcggcagttg ccactcagga cagggaaacc
    19081 actggtacca aaaaatcagt gccgcgaccg cagcggcaga gagccataaa ctttgcgaga
    19141 gaatggcaac aggatccatc agcaaaattg ccgccagaca acatatccat acatcccagc
    19201 cactccactg tcgcccactt aacttaagca ttccccacgt agcaagcgcc accacggtac
    19261 gcaatgcagg aggttgcatt cccgtcaacc aggcataaaa agcagcacag cagattccac
    19321 caattaatgg catttgccag tggatccagc gcccaggcag ataaacttgc ccaccgcgaa
    19381 tgagtccggc agccagcaac gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat
    19441 gcgccgttcc agtatcacgc atgatatttt tgatttcttt ggggacggat aaccgttccc
    19501 ccatacctaa accaagaata accgcattcc acatataggg ttgcagcgtt gtttgtagtg
    19561 acgccagata ctgtgcacgc aggctacaat tcggttcaat gacacttgcc tgcagaaagc
    19621 ggccggtgag cggctgatgc tgggcaatgg cataacgctg gctatcaaag ccgccatcat
    19681 taagttggcc atgaactgca cgaactttga gtttcattga ccattgttgt ccagcacaaa
    19741 ccgcttgcgg aagatattcg ccatacagca caaggcctgg cgcagggaat atacgtcgac
    19801 cttgtagatg agtaatttga ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa
    19861 caattgcatc ctgcgtcgcg ccagttaagg tttctcctgc ccacaaaatt tgctttgctg
    19921 ataatatgcc ccaaacgaaa aacagcaacg tcagcgcgat ataacggacg gttttaacag
    19981 ggataaatgc cagtacgcag gcgaagagag tcagaaacgc aagggttaat gtcccaggca
    20041 attggggcaa aatcagcaac ggaaaaattc cgcaaattat gcatacgccg actgtcgtta
    20101 ttttcatgca cccctccctg tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt
    20161 gggaattttg cgttccccga ggcacattca gaagaattta acgaaagggt gaaaactgct
    20221 caaaactctg cgaggcagct tccagttcct gattagagaa atgaaaaaag cacccgacag
    20281 gtgcttttct cgcgttcaag tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg
    20341 cagttcttta ccaggtttaa agtgaggaac gtattttcct tccagttcta ctttatcgcc
    20401 agtcttcgga ttacgtccgg tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc
    20461 gcggatttca atacgctcgc cctgcgcaag agtcgaggcc atatgctcca gcatctcttt
    20521 tactgcatct tcaaccgtct tggcgggaat gtgcgattgc tgggtggcaa gtctttctat
    20581 caattctgac ttggtcatga ttcctccggt tccttaaagg caaattaagc ggctgctgtt
    20641 gctgcattga ttaagggcgg ccgtagccgc ccttagtgct tgattacagg acgaaacctg
    20701 caatctgtca agtaaactca acaaacttcg gaataaaaat cccgaagagt cagagaatta
    20761 ctcgccttta gctgctttga aagcttcagc cattgcgttg ttggagaagt ttgcatcttc
    20821 ctgtttgtta acagttgcga ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga
    20881 caggctgatt gcgcggtttt tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc
    20941 aacgctcaga accagggtag cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag
    21001 gtaaccttca acgccgtcag ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac
    21061 tttaccggtt acgatagcgc ctttcttgtt cagagcaacc cagttgttga acggatcttc
    21121 tgcgagctgt ttaacgccca gggagatacg ttcacgttct gcgtcaacct gcagaacaac
    21181 tgcagcgatt tcgtcgcctt ttttgtattc acgaactgct tcttcgcctg caacgttcca
    21241 ggagatgtca gacaggtgaa ccaggccgtc gatgccgccg tccaggccga tgaagatacc
    21301 gaagtcagtg atagacttga ttttaccttc aacacggtcg cccttgttgt gggtttccgc
    21361 gaactgctgc cacgggttag ctttgcactg tttcagaccc agggagatac gacgacgttc
    21421 ttcgtcgata tccagaacca taacttccac tacatcgcca acgttaacaa ctttggacgg
    21481 gtggatgttt ttgttggtcc aatccatttc ggaaacgtgt accaggcctt caacgccttc
    21541 ttcgatttca acgaagcagc cgtagtcggt caggttggtc acgcgaccag tcagtttggt
    21601 accttccgga taacgtttag cgatagctac ccacggatct tcgcccaact gtttcaggcc
    21661 cagggataca cgggtacgtt cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc
    21721 cacgttgacg atttcgctcg gatgcttaac gcgtttccag gccatgtcag tgatgtgcag
    21781 caggccgtca acgccgccca gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat
    21841 acctttaact tccatgcctt cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt
    21901 ttcggattcg ataacggcac gacgagaaac aacaacgttg ttgcgcttct gatccagctt
    21961 gattacttta aattcaagct ctttgccttc caggtgcaga gtgtcacgca ccggacgaac
    22021 gtctaccaga gaacctggca ggaacgcacg aataccgttc agctcaacag tgaagccgcc
    22081 cttaactttg ccgttgataa caccggtaac agtttcagca tcttcgtaag ctttttccag
    22141 cgtgatccag gcttcgtgac gtttagcttt ctcacgggac agcagagttt caccgaagcc
    22201 gtcttctact gcgtccagag caacgtcaac ttcatcacct acctggattt ccagctcgcc
    22261 ctgggcgttt ttgaactgct cagccgggat ggcggactca gatttcagac cagcgtcaac
    22321 cagtactacg tctttgtcga tagcaacaac aacgccacga acgatagaac ccgggcgggt
    22381 ttcgatttct tttaaggact cttcaaagag ttgagcaaaa gattcagtca tgtttaatct
    22441 tcaggtttat atttaacgtc cacctggctc catgccggat ggggttgttt aacatacccg
    22501 ctgtcattcc attgcaacgg gggtactgca aattcggtcg cttatgcgag agccaatttc
    22561 tggcgcgcgt attgtagcgc tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc
    22621 aacactaaag catcggctgc cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg
    22681 tcgcgttctt tgatctcggc caaaaggcgc tcaaagttaa cactaaagcc cttctcctgc
    22741 aactgtagca tgcggcgatg cgcacgttct tccgaggagg cgtcaaggaa aattttcact
    22801 ggcgcatcag ggaataccac cgttcccatg tcgcggccat cggcaatcag gccgggtaat
    22861 tcgcgaaacg cgcgttggcg acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact
    22921 tgtgaagctg cattcgccac ttcctgagta cgaatttcgc cgctgacatc ttccccttcg
    22981 aggatcactt ccagattgcc attggttgac acaaaacgta catccagatg ggatgccagc
    23041 ggtaccagcg catcttctga cgcaacatca acatggtgat gtaatgccgc caatgccagt
    23101 acgcgataaa ttgcacccga atccagcaga tgccattgca atgcttccgc catagcctta
    23161 cacaaggtgc ctttccctgc accgcttggg ccatcaatgg taataaccgg ggcaattgcc
    23221 gtcatcttta tctccttaaa acaggcgtac cgttaacata accgcgcgca ttatacgcgc
    23281 cgacgccaca aatcgttaac gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg
    23341 atttattgcg caattatagc gggctggcag aagaccagcc cgaaagatga caatgattta
    23401 cttaccagag gcgatacgat cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga
    23461 gtcaaacatg gactgggtgc gaccaccatc cagactagcc agtttttcaa agctgccaac
    23521 cagcccctgg gtgctgatgc cacgtttctt caacagatca aaggagaaat catctgcttc
    23581 ggactcttta tcacgggagt atttagcatt aatagcgcct tctgcgatat cacccaattg
    23641 tgagcgggaa agctgggaag ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac
    23701 gatcgcataa gaagctttca tttcagccag cgagtgaccc aacgcgacgt ggcccagttc
    23761 atggcccaga acaccttcaa tttcgttgtc attcatcatg tccatcaggc cactgtagac
    23821 acggacacag ccgttcgcca tcgcccatgc gttgacgtcg ctggtcatat aaaccttata
    23881 gttgaccggc gtgccattaa tattgttacc caatgctttg gcgatttttg ccagacgttt
    23941 accgtacttg ctggatttac ttgcgacttg attgccgctg tccatttcgg cacagccctg
    24001 attggcaatc gcttttgcat cggcatcgct taatgttgct gctttatagg catttaaacc
    24061 ggagctgata gccatattgg tatcaatacc gtgggtattt tgacaccctg tcagtaatgc
    24121 tgcagaggtc gcaatcgcca gcagtaattt agtattcttc atgtgaatca tccgttgatt
    24181 aatcatatat aaatattatg tatcaacaac ttacattgac aagcgacgct accattaatc
    24241 aaaatgactc acaaggtccg aaaaaaaacg ccgacaatgt gccgacgttt tgataaagaa
    24301 agatttggct atttattgcc cgttgttcat tcaggctgcc tggctaatcc gcgccaactg
    24361 ctcgaaataa tccggaaatg ttttggccgt acatttggga tcaagaatcg tcactggtgt
    24421 atctgacaac gccaccagcg agaaacacat cgccatccgg tgatcattgt acgtcgcgat
    24481 ctcggcaaag ttcagttttt ccggtggagt gatacgaatg taatcgtgcc cctcttctac
    24541 ttccgcaccg actttacgca gttctgttgc catcgcaaac aggcgatcgg tttctttaac
    24601 acgccagtta tagatattgc gcagcgtggt ggtgcctttt gcaaataacg ccgccgtggc
    24661 aatggtcatc gccgcatcgg gaatatggtt catatccata tcaatagcgt tcagttcacc
    24721 acgcgtgcag gaaatataat catcgcccca gcaaatggtc gcgcccattt tttccagcac
    24781 atcagcaaag cgaatatcac cctgcatact gttacgtcca ataccggtca cttttacagt
    24841 gccgcctttg attgctgctg ctgccagaaa gtaagaagcc gaagatgcat cgccttcgac
    24901 caaataagta cccggagact gataagactg cccgcctttt acgacaaatt gttgatagtg
    24961 ctgattttca atttcaacac caaacgtctt catcagattg agtgtgatgt cgatataagg
    25021 tttagaaacc agatcgcctt taatacgaat caccgtatct tccggcgcaa gaggcgcagt
    25081 cattaacagt gcggtgagga attggctgga aacggagcca tcaacgtcaa cgttgccgcc
    25141 agtaaagccg ccctgtaaac gcaacggcgg ataattttct tgttccaggt aagtgatctt
    25201 cgccccgccc aggcgcagcg catccaccag atgaccaatc gggcgttctt tcatacgcgg
    25261 ctcaccggtc agcacaatat cattgctacc cagacaaaga gctgccgcca gcggacgcat
    25321 tgccgttccg gcgttaccga ggaacaactc cagggcacct tctgcgtgta atggaccgcc
    25381 gttaccgata atttcgcaac gcgtacgatc ggctgaaagc gtatagctta cccctaacgc
    25441 tgttaatgca ttcagcatat ggcgcacgtc atcgctatcc agcagattgg ttaatactgt
    25501 tttgccgtgt gctaatgccg ccagcaataa agcgcggtta gaaacgctct tggaaccggg
    25561 cagattaata gtgccatcga cacgagcgat gggttgtaac gtcagggatt ccatgaactc
    25621 aactctctac aacagaaata aaaaccccac aggctggctg tggggattaa gcaaaatttc
    25681 ggcattaacc gtgacggcgt tcgaactcaa ccatgaagtc tgtcagcgct ttaacgcctt
    25741 ccagcggcat ggcgttataa atagaagcgc gcattccgcc gaccacacgg tgacctttca
    25801 gtgcatgaag gccagcagca aaagactctt caaggaacaa tttgtcaagc gcactgtccg
    25861 ccaactggaa cggcacgttc atccgcgaac ggttagcttt cgccacgtca ttgcggtaga
    25921 aatcgctgtt atcaatcacc ccatatagca gttctgcttt ttgctgattg attttatcca
    25981 tttcagctac accgccgttc gctttcagcc atttaaagac cagaccagat agataccagg
    26041 caaatgtcgg cggcgtgtta aacatggagc cgttatcgtt gaggatggaa taatccagaa
    26101 tcgacggaca cgcgatattc gctttgccca gcaaatcttc acgaacgatg acgattgtca
    26161 ggccagccgg gccgatattt ttctgcgcgc cagcgtaaat cacgccataa cggctgacgt
    26221 caatcggacg ggaaagaatg gttgaagaga agtcggcggc gaccaccaca tctttgccga
    26281 agtctggcgt ttcgtcgatg gcgataccgt cgatggtttc attcgggcag taatgcatat
    26341 aagcagcatt atcagagagt tgccattcac gcattggctt aaccgcgcgc agaccatcaa
    26401 cagtcacttt ggcgtcaaag acattaggcg tgcagtattt tttcgcttct ttaatggcac
    26461 ttgccgccca gtaaccggca tcaacataat ctgcggtggt tttatcaccg agaatattca
    26521 gcggtaccgc agcaaactga ccgcgaccac cgccatggca gaataatacc ttgtagttgg
    26581 aggggacatt aagaagatcg cgaaaatcct tctcggcttc ctctgcaacc tgaatgaact
    26641 ctttgccacg gtgactcact tccatcaccg acgtaccaag accgttccag tcgcgcagtt
    26701 cctgttgagc ctgtttaagc acctctgccg gtagcattgc cggaccagaa ctaaaattga
    26761 agatttgagc catttcccct caccacgttg cgttgctatt aacccgctgt caatcgattg
    26821 cgaccacagg taatacatta ttcctgtggc tatcggtttt atcattcagt gacacacatc
    26881 gcaatgagta aaaccgttga gggcaaaaat gtggcttgca tcacacaaaa gaatctctcg
    26941 gattattagc aaaaagccga tgtttttact gtcaggtcac ccgttttgcc cgacttgcct
    27001 tcgtccattc tggatttgca cagcgcggac ataattgttt ttcccccgct ttcatatgaa
    27061 tgatttcact acaacgaata caggcatagt ccgccgactc cggcaccact ttgtaacgct
    27121 gcgtacaatc actgggtaaa ataagtaaac ccggtttcac cttgtcatct tcaaagaaat
    27181 agacactgat ttgcccgttg gtttcgagaa tcgccagacg tacctgcccc agctgctcca
    27241 cgccacgcaa tcgtagctcc ataaagaact caaattccgt catgttggag ttattgagtt
    27301 tcgaccaggc cagctcgcca tcttcaataa taacaaccgg cttaccttcc agtagatctt
    27361 ccagtttttc actgtgcgcc atcaaccaca ttaccaggcg gtataacaac gccagagtga
    27421 taaagacgat aagtaccggc accatcggca catcatcata aaacgccaca tctcccgccg
    27481 ctgaacccag cgtgagaatg attaacactt caaacagcga catctgtcgc acaccgcgtc
    27541 ttccggtcat tttgaggaac aaaaaaacca gtacaaaggt ataaagacta cgtagtgcaa
    27601 cttcgccaag gaaatcaaaa ggcactttat caaatgccat acggtggaga tcgaacgcct
    27661 tcatttttat gctcttatcg tggtttatcc gctaaaagca tagtcaacag ttcatcggca
    27721 tggcggcggt gatagcgcct ttgagataaa acccgtatta ttgcgcgctt tccgtacgac
    27781 taaagtgatt ttcatgacgc aaacatttat ccccggcaaa gatgccgctc tggaagattc
    27841 catcgctcgc ttccagcaaa aactttcaga cctcggcttt cagattgaag aggcctcctg
    27901 gctgaatcct gtgcctaacg tctggtctgt acatattcgc gacaaagagt gcgcactgtg
    27961 ttttaccaac ggaaaaggcg caaccaaaaa agcggcgctg gcttctgcac ttggtgaata
    28021 tttcgagcgt ctctcaacca actacttttt tgctgatttc tggctgggcg aaaccatcgc
    28081 caacggtccg ttcgtgcatt atcccaacga aaaatggttc ccactgaccg aaaatgacga
    28141 tgtgccagaa ggactactcg atgaccgtct gcgcgcgttt tatgatccgg agaatgaact
    28201 gaccggcagc atgctgattg acctacaatc cggtaacgaa gatcgtggta tttgcggcct
    28261 gccgtttacg cgtcagtccg acaatcagac cgtttatatt ccgatgaata tcattggtaa
    28321 cctgtacgtc tccaacggta tgtccgcagg taatacccgc aacgaagcac gcgttcaggg
    28381 attgtctgaa gttttcgaac gctacgtgaa aaaccgcatt attgctgaaa gcatcagcct
    28441 gccagagatc ccggcagacg tgctggcgcg ttacccagca gtagttgaag ccatcgaaac
    28501 gctggaagcg gagggtttcc caatcttcgc ttatgacggt tcgcttggcg gccagtatcc
    28561 ggtgatttgc gtggtactgt tcaatcctgc taacggtacc tgctttgcct ctttcggtgc
    28621 gcatcctgat tttggcgtag cactggaacg taccgtgacc gagctgctgc aaggtcgtgg
    28681 cctgaaagat ttggatgtgt ttactccgcc aaccttcgat gatgaagaag tcgctgaaca
    28741 taccaacctc gaaacgcact ttatcgattc cagcggttta atctcctggg acctgttcaa
    28801 gcaggatgcc gattatccgt ttgtggactg gaatttctcc ggcaccacgg aagaagagtt
    28861 tgctacgctg atggctatct tcaacaaaga agataaagaa gtttatattg ccgattacga
    28921 gcatctgggc gtttatgctt gccgtattat cgtgcctggc atgtccgata tttatccggc
    28981 tgaagatctg tggctcgcga ataacagtat gggcagccat ttacgtgaaa cgattctttc
    29041 gctaccaggc agcgagtggg aaaaagaaga ttacctgaac ctcatcgagc aactggatga
    29101 agaaggtttt gatgacttta cccgcgtgcg tgagctgttg ggtctggcga ccgggtcgga
    29161 taacggttgg tacaccctgc gtatcggtga attaaaagcc atgctggcgc tggctggtgg
    29221 cgatctggaa caggctctgg tctggaccga atggacgatg gagtttaact catcagtatt
    29281 tagtccggaa cgcgccaact attatcgctg cctgcaaacg ttgttattac tggcacagga
    29341 agaagatcgc cagccgctgc aatatctgaa tgcgtttgtt cgcatgtacg gcgcagatgc
    29401 cgtagaagcc gccagtgcgg caatgagcgg cgaagcggcg ttttacggcc tgcaaccggt
    29461 agatagcgat ctgcacgcgt ttgctgcaca tcagtcgttg ttgaaggcct acgaaaagct
    29521 gcagcgcgcc aaagcagcat tctgggcaaa ataaaatcaa atagcctacg caatgtaggc
    29581 ttaatgatta gtctgagtta tattacgggg cgttttttta atgccccgct ttacatatat
    29641 ttgcattaat aaaataattg taattataag gttaaatatc ggt
//
LOCUS       DAZGJW010000052        29677 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZGJW010000052 DAZGJW010000000
VERSION     DAZGJW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.052"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(79..1908)
                     /gene="glmS"
                     /locus_tag="ACHKIK_002282"
     CDS             complement(79..1908)
                     /gene="glmS"
                     /locus_tag="ACHKIK_002282"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO9083445.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2070..3440)
                     /gene="glmU"
                     /locus_tag="ACHKIK_002283"
     CDS             complement(2070..3440)
                     /gene="glmU"
                     /locus_tag="ACHKIK_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO9083446.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3791..4210)
                     /gene="atpC"
                     /locus_tag="ACHKIK_002284"
     CDS             complement(3791..4210)
                     /gene="atpC"
                     /locus_tag="ACHKIK_002284"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO9083447.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4231..5613)
                     /gene="atpD"
                     /locus_tag="ACHKIK_002285"
     CDS             complement(4231..5613)
                     /gene="atpD"
                     /locus_tag="ACHKIK_002285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO9083448.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5640..6503)
                     /gene="atpG"
                     /locus_tag="ACHKIK_002286"
     CDS             complement(5640..6503)
                     /gene="atpG"
                     /locus_tag="ACHKIK_002286"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO9083449.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YADTMRKVIGHLASGNLEYKHPYLEERDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6554..8095)
                     /gene="atpA"
                     /locus_tag="ACHKIK_002287"
     CDS             complement(6554..8095)
                     /gene="atpA"
                     /locus_tag="ACHKIK_002287"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO9083450.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLVDVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8108..8641)
                     /gene="atpH"
                     /locus_tag="ACHKIK_002288"
     CDS             complement(8108..8641)
                     /gene="atpH"
                     /locus_tag="ACHKIK_002288"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO9083451.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8656..9126)
                     /gene="atpF"
                     /locus_tag="ACHKIK_002289"
     CDS             complement(8656..9126)
                     /gene="atpF"
                     /locus_tag="ACHKIK_002289"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO9083452.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9188..9427)
                     /gene="atpE"
                     /locus_tag="ACHKIK_002290"
     CDS             complement(9188..9427)
                     /gene="atpE"
                     /locus_tag="ACHKIK_002290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO9083453.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9474..10289)
                     /gene="atpB"
                     /locus_tag="ACHKIK_002291"
     CDS             complement(9474..10289)
                     /gene="atpB"
                     /locus_tag="ACHKIK_002291"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO9083454.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10298..10678)
                     /gene="atpI"
                     /locus_tag="ACHKIK_002292"
     CDS             complement(10298..10678)
                     /gene="atpI"
                     /locus_tag="ACHKIK_002292"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO9083455.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11295..11918)
                     /gene="rsmG"
                     /locus_tag="ACHKIK_002293"
     CDS             complement(11295..11918)
                     /gene="rsmG"
                     /locus_tag="ACHKIK_002293"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO9083456.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGH
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11982..13871)
                     /gene="mnmG"
                     /locus_tag="ACHKIK_002294"
     CDS             complement(11982..13871)
                     /gene="mnmG"
                     /locus_tag="ACHKIK_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO9083457.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14249..14692)
                     /gene="mioC"
                     /locus_tag="ACHKIK_002295"
     CDS             complement(14249..14692)
                     /gene="mioC"
                     /locus_tag="ACHKIK_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO9083458.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14782..15240)
                     /gene="asnC"
                     /locus_tag="ACHKIK_002296"
     CDS             complement(14782..15240)
                     /gene="asnC"
                     /locus_tag="ACHKIK_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO9083459.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15392..16384
                     /gene="asnA"
                     /locus_tag="ACHKIK_002297"
     CDS             15392..16384
                     /gene="asnA"
                     /locus_tag="ACHKIK_002297"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO9083460.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16389..17840)
                     /gene="viaA"
                     /locus_tag="ACHKIK_002298"
     CDS             complement(16389..17840)
                     /gene="viaA"
                     /locus_tag="ACHKIK_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO9083461.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17834..19330)
                     /gene="ravA"
                     /locus_tag="ACHKIK_002299"
     CDS             complement(17834..19330)
                     /gene="ravA"
                     /locus_tag="ACHKIK_002299"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO9083462.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19553..21421
                     /gene="kup"
                     /locus_tag="ACHKIK_002300"
     CDS             19553..21421
                     /gene="kup"
                     /locus_tag="ACHKIK_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO9083463.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDTVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21588..22007
                     /gene="rbsD"
                     /locus_tag="ACHKIK_002301"
     CDS             21588..22007
                     /gene="rbsD"
                     /locus_tag="ACHKIK_002301"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO9083464.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            22015..23520
                     /gene="rbsA"
                     /locus_tag="ACHKIK_002302"
     CDS             22015..23520
                     /gene="rbsA"
                     /locus_tag="ACHKIK_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO9083465.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23525..24490
                     /gene="rbsC"
                     /locus_tag="ACHKIK_002303"
     CDS             23525..24490
                     /gene="rbsC"
                     /locus_tag="ACHKIK_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO9083466.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24515..25405
                     /gene="rbsB"
                     /locus_tag="ACHKIK_002304"
     CDS             24515..25405
                     /gene="rbsB"
                     /locus_tag="ACHKIK_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO9083467.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25531..26460
                     /gene="rbsK"
                     /locus_tag="ACHKIK_002305"
     CDS             25531..26460
                     /gene="rbsK"
                     /locus_tag="ACHKIK_002305"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO9083468.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26464..27456
                     /gene="rbsR"
                     /locus_tag="ACHKIK_002306"
     CDS             26464..27456
                     /gene="rbsR"
                     /locus_tag="ACHKIK_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO9083469.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27422..28849)
                     /gene="mdtD"
                     /locus_tag="ACHKIK_002307"
     CDS             complement(27422..28849)
                     /gene="mdtD"
                     /locus_tag="ACHKIK_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO9083470.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPVMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(28872..29564)
                     /gene="yieP"
                     /locus_tag="ACHKIK_002308"
     CDS             complement(28872..29564)
                     /gene="yieP"
                     /locus_tag="ACHKIK_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO9083471.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7147 a         7611 c         7569 g         7350 t
ORIGIN      
        1 gaaagataga tgaatttatt gatgtcagtt ataaaaaagg gccaccggtc ggcagccctt
       61 ttttatctat gatagtgatt actcaaccgt aaccgatttt gccaggttac gcggctggtc
      121 aacgtcggtg cctttgatca gcgcgacatg gtaagccagc agctgcagcg gaacggtgta
      181 gaagatcggt gcaatcacct cttccacatg cggcatctcg atgatgtgca tgttatcgct
      241 acttacaaaa cccgcatcct gatcggcgaa gacatacaac tgaccgccac gcgcgcgaac
      301 ttcttcaatg ttggatttca gtttttccaa cagttcgttg ttcggtgcaa cgacgataac
      361 cggcatatcc gcgtcaatca gcgccagcgg gccgtgtttc agctcgcctg cagcgtaggc
      421 ttcagcgtga atgtaagaga tctctttcag cttcaatgcg ccttccagcg cgattgggta
      481 ctgatcgcca cggcccagga acagcgcgtg atgtttgtca gagaaatctt ctgccagagc
      541 ttcaatgcgt ttgtcctgag acagcatctg ctcaatacgg ctcggcagcg cctgcagacc
      601 atgcacaatg tcatgttcaa tggaggcatc cagacctttc aggcgagaca gcttcgccac
      661 cagcatcaac agcacagtta actgagtggt gaatgcttta gtggatgcca cgccgatttc
      721 cgtacccgcg ttggtcatca gcgccagatc ggattcgcgc accagagaag aacccggaac
      781 gttacagatt gccagcgaac caaggtaacc cagctctttc gacagacgca ggcccgccag
      841 ggtatccgcg gtttcgccag actgtgacaa ggtgatcatc aggctgttac gacgcacggc
      901 agatttgcga tagcggaatt cagaggcgat ttcgacgtcg cacggaatac ctgccagcga
      961 ttcaaaccag taacgggaaa ccataccgga gttataagaa gtaccacagg cgaggatctg
     1021 aatatgctca accttcgaca gcagttcgtc ggcgttcggt cccagctcgc ttaaatcaac
     1081 ctgaccgtgg ctgatgcgcc cggtaagggt gtttttgatc gcgttcggct gttcgtagat
     1141 ctctttctgc atgtagtgac ggtaaatacc tttatcgccc gcgtcatatt gcagattgga
     1201 ttcgatatcc tgacgtttta cttccgcgcc agttttatcg aagatgttta ccgaacggcg
     1261 agtaatttcc gcaatatcgc cctcttcaag gaagataaag cgacgggtca ccggcagcag
     1321 cgccagctgg tcagaagcga taaagttttc gcccataccc agaccaatca ccagcggact
     1381 accagaacgt gccgccagca gggtatccgg gtgacgggag tccatgatca ctgtaccgta
     1441 cgcaccacgc agttgcggga tagcacgcag aacagcctca cgcagagtac cgccctgttt
     1501 cagctcccag ttcaccagat gggcaattac ttcggtgtct gtttcagaaa cgaaggtata
     1561 gccacgcgct tttaactctt cgcgcagcgg ttcatggttt tcgatgatgc cgttatgcac
     1621 caccacaatg tgttcagaaa catgcggatg cgcattcact tctgaaggtt caccgtgggt
     1681 cgcccagcga gtatgagcaa tacccgtacc gccatgcaga ggatgttctt ccgctgcctg
     1741 agccagcatc tggactttac cgaggcgacg caggcgggtc atatgacctt ccgcatcaac
     1801 aacggccaga ccggcagagt catatccgcg gtattccaga cgacgtaaac cttcaagaag
     1861 gatttctgct acatcacgtt gcgcgatcgc gccaacaatt ccacacatag tttttgattc
     1921 cgatttatat cgttgtcggt caacctgtat gcccgttttt tcgggcgccc cgagccttgt
     1981 agagagtggg gttattttta tagttactgc ttgtgggagg gagattatgt tatctcctca
     2041 tcccatgtga ccggattacc cggccagaat cactttttct ttaccggacg acgccagcct
     2101 tctttctgag tctgcggcac acggctgata gctaatgcat tttcgccgac attacgcgtc
     2161 acagttgtac ccgcagcaat ggtcgcgcct ttgcctactg ttaccggggc caccagctga
     2221 gtgtcggaac cgacaaacac atcgtcgccg ataatggtct taaatttatt cgcaccatcg
     2281 tagttgcagg taatggttcc cgcgccgatg ttaacgttat cgccaatttc cgcatcgccc
     2341 aggtaagtca gatgaccagc tttcgagcct ttacccagac gcgctttttt catctcaaca
     2401 aagttaccga cgtgtgcacc ttccagcaac tcagcaccag gacgcagtcg ggcaaacggg
     2461 ccaatagtac aggccgctgc cagattcgcg tcttccacaa cggtatacgg actgatttcg
     2521 caatcatcac caatcacgct gtttttaatc acgcaaccgg tgccgatttt cacgcgatga
     2581 ccgagagtca cgttgccctc gatgataacg ttagtatcaa tttcaacatc gcgcccgtga
     2641 gtaagcgtac cgcgcaaatc aaaacgcgcc ggatcgcgca gcataacgcc tgctaacagc
     2701 agtttttcag cctgttcgga ctggtaaacg cgctccagac gggagagttg caggcggtta
     2761 ttcacacctt ctacttcgct taaacgttgc ggatgaacgg cgacgatttc acgcccttcc
     2821 tgatacgcca gcgcaataat gtcggtgatg tagtattcgc cctgagcatt attgttggtc
     2881 agcttcgcca gccagcgttt catatctgcg ccgttagcga tcagaatgcc ggtgttgatc
     2941 tcctgaatct gacgctgctc gtcggtggca tctttgtgct caacaatgcc ggtaactttg
     3001 ccattttcac gggtgatacg tccataaccg gtcggatcat ccagtttcac cgtcagcaga
     3061 ccaatgccac cctgcggttt agcatcacgc aggcgctgga gtgtttcgac agagatcagc
     3121 ggcacgtcgc cgtagagcat taaaatgtct tcatcatcgg caaagaaagg tgcggcctgc
     3181 tgcattgcat gacccgtacc cagctgctct gcctgaagca cccagttaag gttgtcgtct
     3241 ttcagcgcct gtttaagcag atcgccgccg tgaccgtaca ccaggtgaac gtgcgctgcg
     3301 cctaattcat tcgcagcatc aatgacatgc tgaaccatcg ctttcccggc aagggtatgc
     3361 agcactttcg gaagatcgga atacatgcgc gtgcctttgc ctgcggcaag gatcactacg
     3421 ctcatagcat tattcaacat acgcgtcctg actgtaattt gagaacgaat ttaaaccgct
     3481 tcaccttgaa aaaactacat ttttttcatc gtgaaatgga cagaggataa attgttcaat
     3541 aacggattat ccccgctgac gaaagtgcca ttttcgacca tcgtcacgcc ttttgtctgc
     3601 agaaaattaa ggcagtacct tcatttaagc gtcaggtgga tgtttttgct cttatttcga
     3661 tcaatgaata aacgggaaaa caggggtgtt ttatatcagc aggatctatg tgaacgctct
     3721 tcaggacgga tcacgcgtaa aaaaaagcca gcctgtttcc agactggctt ttgtgctttt
     3781 caagccggtg ttacatcgct tttttggtca actcgataac gcgcagctgc gcgatcgctt
     3841 tggccagttc cgcagacgcc tgagcgtaat ctacgtcgcc gtgagagctg ctaatgtgct
     3901 cttcagcctt acgtttcgct tccatggctc gcgcttcgtc gagatcctgg ccgcgaattg
     3961 cggtgtcggc cagaacggtc acgttgccag gctgcacttc aagaatgccg ccagacagat
     4021 agataaactc ttcgtgaccg tgctgtttca cgatgcgaat cataccaggc ttaatggcgg
     4081 tgagcagcgg tgcgtggcca gggtagatcc ccagttcacc ttcgctaccc gttacctgga
     4141 ttttctcgac cagaccagag aacatttgtt gctctgcgct gacgacgtcc aggtggtaag
     4201 tcattgccat atcaccctcc gattaaggcg ttaaagtttt ttggcttttt ccacagcttc
     4261 ttcgatggaa ccgaccatgt agaacgcctg ctccggcagg tgatcgtatt cgccttccat
     4321 gatgccttta aagccacgga tggtgtcttt cagggagacg tatttacccg gagaaccggt
     4381 gaatacttct gccacgaaga acggctggga caggaagcgc tggatcttac gagcacgcgc
     4441 taccaccagt ttgtcttctt cagacagttc atccataccc aggatggcga tgatgtcttt
     4501 cagttcctga taacgttgca ggatggactg aacgccacgc gcggtgtcgt agtgttcctg
     4561 accaaccacc agcgggtcca gctgacggct ggtggagtcc agcgggtcaa cggccgggta
     4621 gatacccaga gacgcgatct gacggctcag taccacggtt gcgtcaaggt gcgcaaaggt
     4681 ggttgccgga gacggatcag tcaagtcatc cgcaggtacg tataccgcct gtacggaggt
     4741 gatagaacca gttttggtgg aggtgatacg ttcctgcaga acgcccatct cttccgccag
     4801 ggtcggctga taacctaccg ctgaaggcat acggcccagc agtgcggata cttccgtacc
     4861 ggccagggtg taacgataga tgttgtcaac gaacagcaga acgtcacgac cttcgtcacg
     4921 gaatttctca gccatggtca gaccggtcag agcaacgcgc agacggtttc ccggcggctc
     4981 gttcatctgg ccatacacca gggatacttt gtcgataacg ttggagtcgg tcatttcgtg
     5041 gtagaagtcg ttaccctcac gagtacgttc acctacgccc gcaaacacag agtaaccgga
     5101 gtgctcgatc gcgatgttac gaatgagctc catcatgttt acggttttac ctacacccgc
     5161 accaccgaac agaccaactt taccgccctt agcgaacgga cacatcaggt cgataacttt
     5221 gataccggtt tccagcagtt cctgagagtt tgacagctct tcgtaggaag gtgctgcgcg
     5281 gtgaatcgcc caacgctctt cttcaccgat ctcgcctttc atgtcgaccg gttcacccag
     5341 tacgttcatg atacggccca gagtcgcttt acctaccggg acttcgatcg ggtgttcgag
     5401 gtcttttaca tccagaccgc gacgcagacc gtcggaggaa cccattgcga tggtacgcac
     5461 gataccgccg ccgagctgct gctgaacttc cagcaccaga cgctcattac cattttgcac
     5521 ctcaagagca tcgtacacgc gcggtacggc atcctgaggg aattcgacgt caactacggc
     5581 gccgattacc tggacaatct ttccagtagc catcttaaat cctctacgaa ataacctgtt
     5641 taaaccgcgg cggcccccga gacgatctcg gtgagttcct gagtaatgct ggcctgacga
     5701 gctttgttgt ataccaactg cagctcttta atcaggctgc cgccattgtc ggtcgcggct
     5761 ttcatcgcca ccatacgggc ggcctgctcg ctggccaggt tttcaaccac gccctgataa
     5821 acctgagatt cgacataacg acgcagcagg gtatccagca acgccttcgg atcgggttcg
     5881 tacaggtaat cccaggattt atgtttcaga tcatcatcat ctgatgccgg taacggcagc
     5941 agctggctga tggtcggaac ctgagacatg gtgttaataa atttgttgct gacaatgtaa
     6001 agcttgtcca gacggccttc gtcgtaggcc tgcaacatca cttttaccgg accgatcagt
     6061 tcggacaggg aagggttatc ccccatgccg gtgacctggg caacaacatt gccgcccacg
     6121 gagttgaaga acgacacgcc tttcgagccg atcattgcga ggtcgcattg aacgcctttg
     6181 tcggtccagg tcttcatttc cgccagcagt tttttgaaca ggttaatgtt caaaccaccg
     6241 cacaaaccac ggtcggtcga caccaccagg tagcccacgc gtttaacgtc gcgttcttcc
     6301 aggtatgggt gcttatattc cagattaccg ctcgcaaggt gaccaatcac tttgcgcatg
     6361 gtatctgcat aaggacggct ggccgccatg cgatcctgcg atttacgcat tttggaagcg
     6421 gcgaccatct ccatcgcttt agtgatcttt tgcgtgttct ggacgcttgc gatcttacta
     6481 cgtatctctt ttgcgccggc catgagcttc tcctcaatgc cttgcggcct gccctaaggc
     6541 aagccgccag acgttaccag gattgggttg ctttgaagga atcgaggatg cctttcagct
     6601 tgccttcgat ttcgtcgttg tagccaccgg tctggttgat ctcttgcatc aacggagcgt
     6661 gatcacggtc gacgtaagcc agcagagcgg cttcgaagct gccgattttc gacagttcaa
     6721 catccaccag gtaaccacgt tctgctgcga acagaaccag agactgttgc gctacggaca
     6781 tcggcgcata ctgtttctgt ttcagcagtt cggtcacttt ctgaccgtgg tcaagctgct
     6841 tacgtgttgc atcgtcaagg tcggatgcaa actgagagaa cgctgccagt tcacgatact
     6901 gtgccagagc ggtacggata ccaccggaca gttttttcat gatcttggtc tgtgctgcac
     6961 caccaacacg ggatacggaa atacccgggt taaccgcagg acgaataccg gcgttgaaca
     7021 ggttggtttc caggaagatc tgaccatcgg taatggagat tacgttggtc ggaacgaacg
     7081 cagaaacgtc acccgcctga gtttcaataa tcggcagtgc ggtcagagaa ccggttttcc
     7141 ctttcacttc acctttggtg aaggcttcaa cgtattcggc gttaacacgc gcagcacgct
     7201 ccagcagacg agagtggagg tagaatacgt cgcccgggaa tgcttcacgt cctggcggac
     7261 gacggagcag cagggagatc tgacggtaag caacagcctg tttagacagg tcatcgtaaa
     7321 tgatcagcgc atcttcaccg cggtcacgga agtattcgcc cattgcgcaa ccggcatacg
     7381 gagccagata ttgcagtgca gcggattcag acgcggttgc taccacaacg atggtgttag
     7441 ccagtgcgcc gtgctcttcc agtttacgta ccacgttaga aatggtggac gctttctggc
     7501 cgatagcgac atagatacat ttgataccgg aatcgcgctg gttgatgatg gcatcgatag
     7561 ccagtgcggt tttaccggtc tgacggtcac cgatgatcaa ttcacgctga ccacgaccaa
     7621 ttgggatcat ggagtcaacg gctttataac cggtctgtac cggctgatct acggactgac
     7681 gttcgataac gcccggagcg attgcttcta cagcagagaa gccgtcgtga tccagcggac
     7741 ctttaccgtc gattggtgca cccagagtgt taaccacacg gcccagcagg ccacggccaa
     7801 ccggaacttc caggatacgg ccagtacact taactttcat gccttcggca aggtcagcgt
     7861 acggacccat aacaaccgca cctacagagt cgcgctcgag gttcagtgcg atagcgtaac
     7921 ggttacccgg cagggagatc atttcaccct gcatacaatc ggccaggccg tgaatgcgga
     7981 taacaccgtc acttacagaa acaatagtac cttcgttgtg agcttcactc acaacattga
     8041 actgagcaat gcgctgcttg atcagttcgc tgatttcggt ggaattcagt tgcatgctcc
     8101 agtcccctta agactgcaag acgtctgcaa ggcgctcaag acgaccgcgt acgctgccat
     8161 caatgaccat atcacccgct cggatgataa cgcctgccat tacagactta tcgattttgc
     8221 aattcagctt aactttgcgt gacagacgtt tttccatcgc agcagaaatt ttcgcgagct
     8281 gttgttcact cagtgcggca gcggaaatga cgtctacctc agcggtagcc tcactcacgg
     8341 cacgaaggtg aataaactgc tccagaacat ccgggagcgc gttaagacga ccattttcag
     8401 ccattacccg aatcaggttc tgaccgtttt cgtccagttg ctcaccacaa actgcgataa
     8461 acgactcggc gagcgtttct ggcgcaagcg cgccagagag aagctctgcc atttgttcgt
     8521 ttttggttac ctcggcggca aacgccagca tgtcctgcca gcgttctaca ctttggtgtt
     8581 cgacggcaaa gtcaaaagct gctttggcgt aggggcgagc taccgtaata aattcagaca
     8641 tcagcccctc cctccttaca gttcagcgac aagtttatcc acgatgtcgc tgttagcagc
     8701 ttcatccacg gaacgttcga tgatcttctc ggcgccagca acagccagga tagcaacttg
     8761 cttacgcagc tcttcacggg cacgtttacg ctcggcttca atttctgcct gcgcctgggc
     8821 cacgatttta gtacgttcct gttctgcctc agctttcgct tcgtccagaa tctgcgagcg
     8881 gcgtttgttc gcctgctcga tgattacctg ggcttccgct ttcgcttttt tcagctggtc
     8941 ggtcgcgctg gcctttgcaa ggtcaaggtc cttatgtgct cgttctgcgg aagcaaggcc
     9001 gtcagcaatt tctttttgac gtttttcgat ggctgccatt aatggcggcc atacgtactt
     9061 catgcagaac agaacgaaca ggacaaacgc gatggcctgg ccgaggattg ttgcgttaag
     9121 attcacagca caatgcctct atttagttaa cgttctgata ttgctcttta aataaaagca
     9181 acgcttacta cgcgacagcg aacatcacgt acagacccag acctacagcg atcatcggga
     9241 tagcatccac cagacccata acgataaaga actgagtacg cagcagagga atcagatcag
     9301 gttgacgcgc tgcgccttcc aggaatttac ccccgaggat gccgataccg atcgcagcac
     9361 cgattgccgc cagacccatc atcacagcgg cagccatgta cagcagatcc atattcaggt
     9421 tttccatgac agtctccagt ttgtttcagt taaaacgtag tagtgttggt aaattaatgt
     9481 tcttcagacg ccatcgacag atagacgatc gtcagaacca tgaagatgaa ggcttgcagc
     9541 gtaatgatca ggatgtggaa aatggcccac ggcacattca ggatccactg tgaccaccac
     9601 ggcaacagac cagcaatcag aatgaaaatc agctcaccgg catacatgtt accgaacagt
     9661 cgcaaaccaa gtgaaactgg tttggacagc aggcttaccc cttcaaggat taagttgaca
     9721 ggaatgaacg cccagtgatt gaacggctgc agcgtcaact ctttcgtgaa gccgccgatg
     9781 cctttcattt tgatgctgta gaacagaatc aggataaata cgcccaatgc catagacagc
     9841 gttacgttca cgtccgcaga cggaaccaca cgcagtgcag gcagacccag tacatgttca
     9901 gcaatgtacg gcagcaggtc gataggcagt aaatccatca ggttcatcag gaatacccag
     9961 acgaagatcg tcagggccag cggagcaatc agcttgcttt tgccatggta catgtctttc
    10021 acgctaccat taacaaagcc gatcaccagc tcaatcgcgg tctgaaactt acctggcaca
    10081 ccgctggtcg cctttttggc tacgctacgg aataaaacca ggaacaacag acccagcacc
    10141 accgagaaga acatggagtc aatattgatt gtccagaagg tggctggggg gttttgtgga
    10201 tccaccagcg agaatgtacg caggtccagc tgaaggttat tcaggtggtg tcctatgtaa
    10261 tcctgcggcg tcatattttc tgaagccatg atgcctttta ccctttgttg ttaattacag
    10321 ccggtgccag tatctgaacc accagcacca aaacccacgt aacgatcagc ggcaagaata
    10381 ccgcctttaa aaccgccaac gccaccacca gtaacaccaa catcgccaga actttgaaag
    10441 cttcgccaaa tgcgaatgtc caggccaccc ggcctttcgc tggtgtatgc gcctggtgac
    10501 gccaggcaaa tatcataaac aaaacgttag gcagaaagac tgccaggccc ccgcttattg
    10561 cagagacgcc ccagaagggg tctttgaggc tgaacagcaa tccacttgct atcaccacca
    10621 gtaactgaac gagcagaagc ttccgagcaa cgtttcgact cacgagcgac acagacatca
    10681 cgtttttcac tcctgctccc ttcgaggtat gccgcgtgtc gtataaaact ttctttaagg
    10741 cttagagtca agcatcaaaa agcggtcaaa ttatacggtg cgcccccgtg atttcaaaca
    10801 ataagtagcc aaaaggtgaa taaatgttta aatatttttc cagtgcatac aattgcgact
    10861 tttctgctaa ccctgttcga tcatgaaaaa ctgtaaataa cgcgtaaaca ctggtgataa
    10921 agcgtgcttc agatcacata ttgcgcatgt tcgcgcacag catatttatt tacttggcaa
    10981 atgatgcctt tgcaagttta tgatatttca gtctaaaaac agatactgtt ttaataaatg
    11041 acatttacac aacaaaaacc acccattgac atttttaata atgttttaac agccaatgat
    11101 ggttcttagc gccgattttt agcagactga tattttcact aatgacttat tttctgctta
    11161 ccaaaaaaag ccacgttatc ttgttgatgc aaaagagtga acgtggcgtt aaatgtaacc
    11221 agttatatca gtagaaaacc tggttgttgt taacagtcta accggtcaat tttttatgat
    11281 ttttttgata aaaattaaat tttatttgct ttaatcacca ccagatgacg ttcgccatcc
    11341 aaggctggaa cctgaagttt aaccactgat tcgacctgat attcttcggg caacaaagcg
    11401 atttcatctt ccggcatttg ccctttcagc gcgtagaaat ggccttgctc accaggaaga
    11461 tggtggcacc agctcaccat atcgttcaga gaggcaaaag cgcggctaat tacgccatca
    11521 aatggcggct ctgaaggaaa ctcttctacc ctgctctgta ctggttcaat attctccagt
    11581 ttaagctcat gttgcacctg acgaaggaaa cgcacgcgtt taccaaggct atccaacaga
    11641 gtgaaatggg cttcaggacg cacgatagag agtggaatgc ctggcagtcc tggtccggtg
    11701 ccgacatcga taaaccgttc accttgcaga tacggtgcca ccacaatgct atcgagaata
    11761 tggcgtacca gcatctcatt aggatcgcgg accgaagtca ggttgtacgc tttgttccat
    11821 ttatgcagca tattcacgta ggcaataagc tggtttttct ggtgatcggt aagcgaaata
    11881 cctgcgtctt tcagcagtaa ggagagtttg ttgagcacgg tgattacctg ttcttgatgc
    11941 gttgcctggt aagcgggtgc ttaccaggca tttttaatgc gttatgcgct acgacgcagc
    12001 ataccctgtt ttttcagcca caccagcaga atggagatgg ccgcaggcgt gacgccagaa
    12061 atacgcgaag cctggccgat agaggctggt ttgtgatcgt taagtttggc gatcacttcg
    12121 ttagaaagac cggatacctg gcggtaatcc agtgtcgcgg gtagcagggt gttctcgtta
    12181 cgcagctgct tttcgatctc atcttgctgg cgcgcgatat aaccttcgta tttaacctga
    12241 atctcaacct gttccgccgc ctgttcgtct gtcaacgcag gggcaaacgg cgtcagcgtg
    12301 gttaattttt cataagtcat ttccggacga cgcagcagat cttcaccact ggcttcacgg
    12361 gaaagcggcg cagtcaggtg agcattcact tcggctgcag cttccgccga cggggttacc
    12421 caggtcgatt tcagacgctg acgctcacgc tcgatattct caagtttctc gttaaagcgc
    12481 gcccaacgtt cgtcatccac caggcccagt tcacgaccga tttcagtcaa acgcagatcc
    12541 gcattatctt cgcgtagcat cagacgatat tctgcgcgcg aagtaaacat acgatacggt
    12601 tctttggttc ctaaagtgca caggtcatca actagtacgc cgagatacgc ctgagaacgt
    12661 gccggagccc aaccttcttt gtcagcagac agacgggcag cgttaagacc ggccagcaaa
    12721 ccttgcgcag cggcttcttc gtaaccggta gtgccgttaa tctgaccagc aaagaacagc
    12781 ccctggataa acttgctctc cagcgtcggt ttcaggtcgc gaggatcgaa gaagtcatac
    12841 tcaatggcat aacccggacg cacgatcttc gcgttttcca tcccctgcat agagcggacg
    12901 atttgcatct gcacatcgaa cggcaggctg gtggagatac cgttcggata aatttcatta
    12961 gaggtcagtc cttccggttc aaggaagatc tgatgctgat ttctgtcggc gaagcgcata
    13021 actttgtctt cgatcgacgg gcagtagcgt gggccgacac cttcgatcac ccctgcatac
    13081 attgggctac gatcgaggtt actgcggatc acatcatggg ttttctcgtt ggtatgagtg
    13141 atataacacg gcacctgctg gggatgctgg gacgcattgc ccataaacga gaataccggc
    13201 attgggttat cgccatgctg ttgcgccagc acactaaagt cgatagttcg agcatcaata
    13261 cgcggtggtg tcccggtttt cagacgacca acgcgcagcg gcagttcacg caaacggcga
    13321 gaaagcggaa tggacggcgg atcaccagca cggccaccgc tgtaattatc cagaccgata
    13381 tgaattttac cgtcgaggaa cgtcccaacg gtgagcacga cggctttggc acggaacttc
    13441 agtcccattt gggtaacagc accgaccacg cgatcgtttt cgacaataag atcttcaacc
    13501 gcctgctgga agatcatcag gttcggttgg ttctccagcg ccgtacgtac cgcctgacgg
    13561 tagagcacac gatccgcctg agctcgggta gcgcgaaccg ccggtccttt gcttgcgttt
    13621 agtatcctaa actggatacc cgcttgatcg atcgctttcg ccatcagacc gccgagtgca
    13681 tccacttctt ttaccagatg tcccttccca ataccgccga tcgccgggtt gcagctcatc
    13741 tgccccagag tgtcgatatt gtgtgtcaaa agcagagtct gttgacccat acgcgccgcg
    13801 gccatcgcgg cctcggtgcc tgcatgaccc ccgccaatga tgatgacgtc aaaaggatcc
    13861 ggataaaaca tggtgattgc ctcgcataac gcggtatgaa aatggattga agcccgggcc
    13921 gtggattcta ctcaactttg tcggcttgag aaagacctgg gatcctgggt attaaaaaga
    13981 agatctattt atttagagat ctgttctatt gtgatctctt attaggatcg cactccctgt
    14041 ggataacaag gatccggctt ttaagatcaa caacctggaa aggatcatta actgtgaatg
    14101 atcggtgatc ctggaccgta taagctggga tcagaatgag gggttataca caactcaaaa
    14161 actgaacaac cgttgttcct tggataacta ccggttgatc caagcttcct gacagagtta
    14221 tccacagtag atcgcacgat ctgtatactt atttgagtaa attaatccac gatcccagcc
    14281 attcttctgc cggatcttcc ggaatgtcgt gatcaagaat gttgatcttc agtgtttcgc
    14341 ctgtctgttt tgcaccggaa tttttgagtt ctgcctcgag tttatcgata gccccacaaa
    14401 aggtgtcata ttcacgacta ccaataccga ttgcgccaaa gcggaccgca gaaagatcgg
    14461 gcttctgttc ctgcaatgct tcatagaaag gagaaaggtt gtccggaata tctccggcac
    14521 cgtgggtgga gctgataatc agccagatcc ccgaggcaga taaatcttcc aacagcggac
    14581 cgtgcagcgt ttcggtggta aaacccgcct cttccagctt ttcagccagg tgttctgcta
    14641 catattcggc accgccgagg gtgctgccgc tgataagagt gatatctgcc ataaaccgcc
    14701 acctttatta agagtggcgt attgtacgct gtgaacgcgt tgggatctac ctgtggaaaa
    14761 gtatgggatt aaaaaagccg attatggctt gatggtacgc atgatcgggt tttgcaggac
    14821 gatcaatgtc tcggtggact gaatttcatc aattgtttgg atcttgttga taagtacatg
    14881 ctggagagcg tcgatcgaac ggcacatcac ttttataaag atgctgtagt ggccggttgt
    14941 gtaataggct tcagtgactt catcaaggct ttccagcttt gccagcgcgg aagggtagtc
    15001 tttggcgctc tttaatataa tgccgataaa gcagcctacg tcataaccga gctgcttcgg
    15061 gctgacatca atacgcgccc cggtaatgat ccccgcctgc ttcattttct ctactcgaac
    15121 gtgaatcgtc cccggactga cgccaaattg tttcgccagt tcggcgtaag cggtgcgcgc
    15181 attgcccatt aatgcttcca ggatgccacg gtccagattg tcgatcagat aattttccat
    15241 aggattttct tatgcagatt gatgattcat tctattttag ccttcttttt taatgaatca
    15301 aaagtgtgtt aggcttttta ttgaatgatt attgcatgtg tgtcggtttt tgttgcttaa
    15361 tcataagcaa caggacgcag gagtataaaa aatgaaaacc gcttacattg ccaaacaacg
    15421 ccaaattagc ttcgtgaaat ctcacttttc tcgccaactg gaagaacgtc tggggctgat
    15481 cgaagtccag gcgccgattc ttagccgtgt gggggatggc acgcaggata acttgtcggg
    15541 ctgtgaaaaa gcggtgcagg taaaagtgaa agctctgcct gatgcccagt tcgaagtggt
    15601 tcattcactg gcgaagtgga aacgtcagac cttagggcaa cacgacttca gcgcgggcga
    15661 agggctgtac acgcacatga aagcccttcg ccccgatgaa gagcgtcttt ctccgttgca
    15721 ctcggtctat gttgaccagt gggactggga acgcgtaatg ggcgacggtg agcgtcaatt
    15781 ctcgactctg aaaagcacgg tagaggcgat ctgggcggga attaaagcaa ccgaagctgc
    15841 agttagcgaa gagtttggtt tggcaccgtt cctgccggat cagatccact tcgtacacag
    15901 ccaggagtta ctgtctcgtt atccggatct cgatgccaaa gggcgtgagc gggcgattgc
    15961 gaaagatctt ggcgcggtat tcctcgtcgg gattggcggc aagctgagtg atggtcatcg
    16021 tcacgacgtg cgcgcaccgg attatgatga ctggagcacc ccgtcagagc tgggccatgc
    16081 gggtctgaac ggcgatattc tggtatggaa ccctgtactg gaagatgcgt ttgagctttc
    16141 ttccatgggg atccgcgtgg atgccgacac gttgaagcat cagttggcgc tgaccggtga
    16201 tgaagatcgt ctgcagctgg aatggcatca ggcgctgctg cgcggtgaaa tgccgcagac
    16261 catcggcggc ggtatcggcc agtctcgttt gaccatgctg ctgctgcaac tgccgcatat
    16321 cggccaggtt cagtgtggag tatggccagc tgctgttcgc gagagcgtcc cttctctgct
    16381 gtaataattt atcgccgcca gcgtctgagc aggcggcttc gcatcccggt atcaaagcgc
    16441 cagatatgat cgaaaatgcg catgatgccg ggtttgccgt gtgccgacat cgccacggca
    16501 tgaaagcgat gctgatgtac ccgctgcagc tctttcactt tactcgtcac gtcgtcaggc
    16561 aaccgctgag cgataaaatc agaaatcacc accgcatcgg catcaaacca ttccctgctt
    16621 tgcaagcgtt ccataatggc gcgaaaacaa ctggcaagat cggtgccgcc acgaaactgc
    16681 tggcttaaaa aacggattgc ttgttcgatg ccttgtgggc ctgaaagctc ataacggacg
    16741 atctcggtgg aaaatagcat aatatagcag cgccggtttt cagcgagagc aatgcgcatc
    16801 aaggccaggc agaacgcttt cgcacactgt tcattaaagc cgcccattga gccggaagta
    16861 tccacacaga caataaacgg cccgcgcggc tgttcatcgt aatctttatg taccaccgga
    16921 cgttcgatca ctttttcacg ccacgactca ccgtgcaggc gataggtgag caactgtttt
    16981 tccaccagcc gacggtaaaa ctcatactcc agttccgtta tccctagtgt cgccagttct
    17041 ggcggcagaa gacgtaaaat atcatcgctt tgttgcagac catcaacctg ctcaggaacc
    17101 gtcgccggtt cgcgcaccat ggtgcggaag gtttccatct gcgcatcgtt gcgcggtatt
    17161 gatttggctt cccgagaacg ccccagctgc tctgccaggc gtttcagttc cggctgttcg
    17221 ttaagaaatt caccgtattt cacaatcaac tgatagtcgc cacgtttaag ctgaccggcg
    17281 ctcatatccc acagacgacc agctgcagta ttgttatctg cgagaatcgg ttcaagttgt
    17341 ccgctcagcg tcatgcgttc ctgaacttca ctcaacagtt gttcgcgttc ttcttctaat
    17401 agctgttgat ttaacgtcgt tgcttgcacg atcagactta aacgccaacg ctggagaaaa
    17461 agcgtgtgta acgctgaagt gatcgtgctg ttagcatcaa ccaactgtcg ggcttgttct
    17521 gcccatggag aattcagacg atgcagtaag tccaggatct gtggtagctg cacaataaac
    17581 tgtggcgtgg agaggagctg gctttgctga tagcacatca cctcttcggt gagttccggc
    17641 gggactcggg catctttcag ccgactgcgc agcgcttcac gccagcgggg aacatcatca
    17701 gtgattgccg ccttcaggcg tgggaatttt tcaaagaaga ccgccagctg cggtgaggcc
    17761 agcagtgcga tgatcatctc ttcgatcaat ccctcttcgc tgacggccag catcacatta
    17821 agcgtatcca gcgttagcat tgttgtgcct ggcgaatctg tgcaccgaca tcctgtaggc
    17881 tggcttcaat gcgacctaac cagtcgccag ggataaacag acatttttgc tgttcgctga
    17941 ataaagcgtg ttgcttgcgc cagtcgcttt caagctcttc cagttgttgt tttatttcac
    18001 caggcagacc ttcagccgat gaaccgggaa gtgccagcgt actgccttgt aaacttacat
    18061 cgcgaacgac aagatgttgg gcgctatcaa cttccagatt cagtttctgg gcaaagccga
    18121 taccgttcag tttaccgcga atttcaccac ctttgctcag ccactgttcc agcgcgctac
    18181 gctcaaagga gatatgaacc acttccatat catgcagttt taacggtttt tgcagcagta
    18241 gagtcagagt ggaagcagta acgttaacag gaagttgata ctgctgacga cggctgaaaa
    18301 tgccgcccag acgaattacc gttaaggctg ttttatcgct ttgttgctgc tggagttgca
    18361 ggtgacgttg cacaatcgcg ccaaggcgag tcaacatccc ttgctgttgc caggcgtgac
    18421 cggtcatcaa tacatcaatt tgttgttgta tcaaattcag gctttgcgcg tcataccaca
    18481 ggcaatcttt cagcaaaatg agatctaccg gggcaacagc actgcgaccg ctaaaaaagg
    18541 cgctggcctg caataatcgg atcgcttttt tccagcgacg atccgagaca taaggcgcat
    18601 ccggtaattt atccagttgc tggcgcagca taaaaatcag ctcaaataca tgatcgggca
    18661 gcgtaatttc accaatctct ttctgccagc gttcatattc ttcatctgtg acctgcaagg
    18721 catcaggaac cggattgtcg ttttcatcct gttgactggt cagcatggag cggaaattcg
    18781 ctttatcctg cactttatct aaccacagac gaatcagcat gcggtcatat aacgcttcca
    18841 gactgctgtc tgcttccggc agctcgttgg aggccgccac cagcagacgc atcgggattt
    18901 tttctacgtg tgcaccgttg cggaactggc gctcgttaat ggcggtgagc aaggtattaa
    18961 gaattgccgg gcccgctttc cagatctcat ccagaaagac aatttctgct tccggcaggt
    19021 aaccgctggt taaacgttca tagcgccctt catcttttag cgcctgaata gaaaggggac
    19081 caaaaacttc ttccggcgtg gagaagcggg tcatcagata ttcaaacgcg cgggcattct
    19141 gaaaggcgaa ttttaagcgc cgggcgatca aacttttggc aatacctggc gggccaagga
    19201 ggaacacact ttcaccactt aatgccgcta ataaacacaa gcggatggcg tggctacgtt
    19261 cataaagccc cttttccaac gaactgctca ggcgggaaat tctttccgct aataaatgag
    19321 ggtgagccat aatgaagtgg cgtcctttcg tcaaaagttc tgcgtaaatt gcgagtatag
    19381 acgtttcttg ctggtggcta aaatagtctc aaaggggggg tatttttctt tgagccaggt
    19441 taatgtggcc gcatttagga gtacgatttt gccgttaatc gtgcatactg tgcgcttttt
    19501 tgtgggccaa gggactaagc acacatttca tatttcaacg aaagactagt ctatgagcac
    19561 tgataataag caatcattgc ccgcgattac cctcgcggcg attggagttg tctacggcga
    19621 tattggtacc agcccgttat atacacttcg tgaatgtttg tccggccagt ttggttttgg
    19681 cgttgaacgc gataccgtgt ttggcttttt atcgctgatc ttctggctgc taatctttgt
    19741 ggtttccatt aaatatctca ccttcgtgat gcgggcagat aacgccggtg agggggggat
    19801 cctgacgttg atgtcgcttg ccgggcgtaa tacgtcggcg cgaaccacat caatgctggt
    19861 gattatgggg ctaatcggcg gcagcttttt ctatggtgaa gtcgtcataa cacccgctat
    19921 ttcggtgatg tcagccattg aaggtctgga aatcgtcgcc ccgcagctgg atacctggat
    19981 agttcccctc tcaattatcg ttctcacttt attatttatg attcaaaaac atggcacggc
    20041 tatggtcggt aagctgtttg cgccgatcat gctgacctgg tttttgattc tggcaggact
    20101 ggggttacgt agcattattg ctaacccgga agtgctgcat gcgctgaatc caatgtgggc
    20161 ggtgcatttc ttccttgaat acaaaacggt ttcttttatt gcattagggg cagtggtgtt
    20221 gtcgattacg ggggtcgagg cgctgtatgc tgatatgggg cactttggta agttccctat
    20281 tcgcctggcg tggttcaccg tcgtattacc ttccttgacc cttaattact tcggtcaggg
    20341 agcgctgttg ttaaagaacc cggaagcgat taagaacccg ttcttcctgt tggcaccgga
    20401 ctgggcgctg atcccgctgc tgatcatcgc cgcactggcg acggtaattg cctcgcaggc
    20461 ggttatctct ggcgtcttct cattgacgcg tcaggcggta cgtctgggat atttgtcgcc
    20521 gatgcgcatt attcacacct ccgaaatgga gtcagggcaa atctatattc cgtttgtgaa
    20581 ctggatgctc tatgtcgcgg tcgtgattgt gattgtcagc tttgagcact ccagcaacct
    20641 ggcggcggcg tacgggattg cggtgaccgg aaccatggtg ctgacgtcta ttctctcgac
    20701 taccgtggca cgtcagaact ggcactggaa taagtatttt gttgcgctga tcctgattgc
    20761 tttcctttgt gtcgatattc cattgttcac cgctaacctc gataaactgc tctccggcgg
    20821 ctggttgcca ttgagcctcg gtactgtgat gtttatcgtg atgaccacct ggaagagcga
    20881 gcgtttccgc ttgctgcggc ggatgcatga acatggtaac tctctggaag cgatgattgc
    20941 ttcgctggag aaatcaccgc ctgttcgcgt gcccgggacc gcggtgtata tgtcgcgtgc
    21001 aatcaacgtc attccctttg cgctgatgca taaccttaaa cataacaagg tattgcatga
    21061 gcgggtgatt ctgttaactc tgcgtaccga agacgcgcca tatgtccata atgtccgtcg
    21121 ggtacagatt gaacaactgt cgcccacttt ctggcgcgtg gtggcaagtt atggttggcg
    21181 agaaacgcca aacgtggaag aagttttcca ccgctgcggt ctggaaggat taagttgccg
    21241 gatgatggaa acctccttct ttatgtcgca tgagtcgttg atcctcggca aacgcccgtg
    21301 gtatttgcgt ctgcgcggca agctgtactt gctgctgcaa cgtaatgcgc tgcgtgcacc
    21361 agatcaattt gaaattccgc caaacagggt tatcgaactg ggtactcagg tcgaaatcta
    21421 acgccagacg cctcctttct ccataagggg gcgtttttgt tttcatggtt aatcaccatg
    21481 taaaacgttt cgaggttgat cacatttccg taacgtcacg atggttttcc caactcagtc
    21541 aggattaaac tgcgggtcag cgaaacgttt cgctgatgga gaaaaaaatg aaaaaaggca
    21601 ccgttcttaa ttctgatatt tcatcggtga tctcccgtct gggacatacc gatacgctgg
    21661 tggtgtgtga tgctggttta cctatcccca aaagtacaac gcgtatcgat atggcattaa
    21721 cccagggtgt accttctttt atgcaggtgc tgggcgtcgt cacaaatgaa atgcaggtcg
    21781 aggctgccat tatcgcggaa gagatcaaac aacataatcc gcaactccac gaaacgttgc
    21841 tcactcacct tgagcagctg caaaaacacc agggaaatac cattgaaatt cgttacacca
    21901 cgcatgaaca attcaaacaa caaaccgcag aaagtcaggc ggtaattcgc agcggagaat
    21961 gttctccgta tgcgaatatc attctctgtg ctggcgtgac gttctgaggc cgtcatggaa
    22021 gcattacttc agcttaaagg catcgataaa gccttcccgg gcgtaaaagc cctctcgggc
    22081 gcagcgttaa atgtctatcc gggccgcgtg atggcgctgg tgggcgaaaa cggcgcgggt
    22141 aaatccacca tgatgaaagt gcttactggt atctatgctc gcgatgccgg cacgctttta
    22201 tggctgggga aagaaacgac atttaccggg ccgaaatctt cccaggaagc cgggattggg
    22261 attatccatc aggaactgaa cctgatcccg cagttgacca ttgccgaaaa cattttcctc
    22321 ggtcgtgagt ttgttaatcg ctttggcaaa attgactgga aaaccatgta tgccgaagcg
    22381 gataaattgc tggctaaact taacctgcgc tttaaaagcg acaagctggt gggcgatctt
    22441 tccatcggtg accagcaaat ggttgaaatc gccaaagtgc tgagctttga gtcgaaagtc
    22501 atcattatgg atgaaccgac cgatgcgctg accgataccg aaaccgaatc cctgttccgc
    22561 gtcatccgcg aactaaaatc gcaaggccgc ggtattgtct atatctccca ccgcatgaaa
    22621 gaaatcttcg agatttgcga tgacgttacc gtttttcgtg acgggcaatt tattgctgag
    22681 cgcgaagtgg catcactgac cgaagattcg ctgattgaga tgatggtggg ccgcaagctg
    22741 gaagatcaat atccacacct ggacaaagcg ccgggagata tccgcctgaa agtcgataat
    22801 ctctgcggac ctggcgttaa cgatgtctct tttactttac gcaaaggcga aattcttggc
    22861 gtctctggtt tgatgggcgc aggtcgtacc gaactgatga aagtgctcta cggcgcacta
    22921 ccgcgcacca gcggttacgt caccctggat gggcatgaag tcgttacccg ttcaccgcag
    22981 gatggcctgg caaacggcat tgtgtatatc tccgaagacc gtaaacgtga cggtttagtg
    23041 ttgggcatgt cagtaaaaga gaacatgtcg ctgacagcgc tgcgctactt cagccgcgct
    23101 ggcggcagtt tgaagcatgc cgatgaacag caggctgtga gtgatttcat tcgtctgttt
    23161 aatgtgaaaa caccgtcgat ggaacaggca attggtctgc tttccggtgg caatcagcaa
    23221 aaagtggcga ttgcccgcgg tctgatgaca cgccccaaag tgttgatcct tgatgaacct
    23281 acccgtggcg tagatgtcgg cgcgaaaaaa gagatctatc aactgattaa ccagttcaaa
    23341 gccgatggct tgagcatcat tctggtgtca tcggagatgc cagaagtatt aggcatgagc
    23401 gatcgcatca tcgtcatgca tgaagggcat ctcagcgggg aatttactcg tgagcaggcc
    23461 acccaggaag tgttaatggc tgccgctgtg ggcaagctta atcgcgtgaa tcaggagtaa
    23521 aaaaatgaca acccagactg tctctggtcg ccgttatttc acgaaagcgt ggctgatgga
    23581 gcagaaatcg cttatcgctc tgctggtgct gatcgcgatt gtctcgacgt taagcccgaa
    23641 ctttttcacc atcaataact tattcaatat tctccagcaa acctcggtga acgccattat
    23701 ggcggtcggg atgacgctgg tgatcctgac gtcgggcatc gacttgtctg taggttctct
    23761 gttggcgcta accggcgcag ttgctgcatc tatcgtcggc attgaagtca atgcgctggt
    23821 ggctgtcgct gccgctctcg cgttaggtgc cgcaattggt gcggtaaccg gggtgattgt
    23881 agcgaaaggt cgcgttcagg cgtttatcgc tacgctggtt atgatgcttt tactgcgcgg
    23941 cgtgaccatg gtttatacca acggtagccc agtgaatacc ggctttactg agaacgccga
    24001 tctgtttggc tggtttggta ttggtcgtcc gctgggcgta ccgacgccag tctggatcat
    24061 ggggattgtc ttcctcgcgg cctggtacat gctgcatcac acgcgtctgg ggcgttacat
    24121 ctacgcgctg ggcggcaacg aagcggcaac gcgtctttct ggtatcaacg tcaataaaat
    24181 caaaatcatc gtctattctc tttgtggtct gctggcatcg ctggccggga tcattgaagt
    24241 ggcgcgtctc tcctccgcac aacccacggc ggggactggc tatgagctgg atgctattgc
    24301 tgcggtggtt ctgggcggta cgagtctggc gggcggaaaa ggtcgcattg ttgggacgtt
    24361 gatcggcgca ttaattcttg gcttccttaa taatggattg aatttgttag gtgtttcctc
    24421 ctattaccag atgatcgtca aagcggtggt gattttgctg gcggtgctgg tagacaacaa
    24481 aaagcagtaa taacgactac aggacatctt gaatatgaac atgaaaaaac tggctaccct
    24541 ggtttccgct gttgcgctaa gcgccaccgt cagtgcgaat gcgatggcaa aagacaccat
    24601 cgcgctggtg gtctccacgc ttaacaaccc gttctttgta tcgctgaaag atggcgcgca
    24661 gaaagaggcg gataagcttg gctataacct ggtggtgctg gactcccaga acaacccggc
    24721 gaaagagctg gcgaacgtgc aggacttaac cgttcgcggc accaaaattc tgctgattaa
    24781 cccgaccgac tccgacgcag taggtaatgc tgtgaagatg gctaaccagg cgaacatccc
    24841 ggttatcact cttgaccgcc aggcaacgaa aggtgaagtg gtgagccaca ttgcttctga
    24901 taacgtactg ggtggcaaaa tcgccggtga ttacatcgcg aagaaagcgg gtgaaggtgc
    24961 caaagttatc gagctgcaag gcattgctgg tacatccgca gcccgtgaac gtggcgaagg
    25021 cttccagcag gccgttgctg ctcacaagtt taatgttctt gccagccagc cagcagattt
    25081 tgatcgcact aaaggtttga acgtaatgca gaacctgttg accgctcatc cggatgttca
    25141 ggctgtattc gcgcagaatg atgaaatggc gctgggcgcg ctgcgcgcac tgcaaactgc
    25201 cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca ccggatggcg aaaaagcggt
    25261 gaatgatggc aaactagcag cgactatcgc tcagctaccc gatcagattg gcgcgaaagg
    25321 cgtcgaaacc gcagataaag tgctgaaagg cgagaaagtt caggctaagt atccggttga
    25381 tctgaaactg gttgttaagc agtagtttta atcaggttgt atgacctgat ggtgacataa
    25441 atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc agggcacgcg ccaccctaac
    25501 acggtggcgc attttatgga catcccgaat atgcaaaacg caggcagcct cgttgttctt
    25561 ggcagcatta atgctgacca cattcttaat cttcaatctt ttcctactcc aggcgaaacc
    25621 gtaaccggta accactatca ggttgcattt ggcggcaaag gcgcgaatca ggctgtggct
    25681 gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta cgggtgatga cagcattggt
    25741 gagagcgttc gccagcagct cgccactgat aacattgata ttactccggt cagcgtgatc
    25801 aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg gcgaaggtga gaatgtcatc
    25861 ggtattcatg ccggcgctaa tgctgccctt tccccggcgc tggtggaagc gcaacgtgag
    25921 cgtattgcca acgcgtcagc attattaatg cagctggaat caccactcga aagtgtgatg
    25981 gcagcggcga aaatcgccca tcaaaataag actatcgttg cgcttaaccc ggctccggct
    26041 cgcgaacttc ctgacgaact gctggcgctg gtggacatta ttacgccaaa cgaaacggaa
    26101 gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag atgcagcgaa ggcggcgcag
    26161 gtactgcatg aaaaaggtat ccgtactgta ctgattactt taggaagtcg tggtgtatgg
    26221 gctagcgtga atggtgaagg tcagcgcgtt cctggattcc gggtgcaggc tgtcgatacc
    26281 attgctgccg gagatacctt taacggcgcg ttaatcacgg cgttgctgga agaaaaacca
    26341 ttgccagagg cgattcggtt tgcccatgct gccgctgcga ttgccgtaac acgtaaaggc
    26401 gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat ttttagacag gcagaggtga
    26461 cgcttggcta caatgaaaga tgttgcccgc ctggcgggcg tttctacctc aacagtttct
    26521 cacgttatca ataaagatcg cttcgtcagt gaagcgatta ccgccaaagt tgaagcggcg
    26581 attaaagaac tcaattacgc gccatcagcc ctggcgcgta gcctcaaact caatcaaaca
    26641 cataccattg gcatgttgat cactgccagt accaatcctt tctattcaga actggtgcgt
    26701 ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg tcctttgcaa taccgaagga
    26761 gatgaacagc ggatgaatcg caatctggaa acgctgatgc aaaaacgcgt tgatggcttg
    26821 ctgttactgt gcaccgaaac gcatcaacct tcgcgtgaaa tcatgcaacg ttatccgaca
    26881 gtgcctactg tgatgatgga ctgggctccg ttcgatggcg acagcgatct tattcaggat
    26941 aactcgttgc tgggcggaga cttagcaacg caatatctga tcgataaagg tcatacccgt
    27001 atcgcctgta ttaccggtcc gctggataaa actccggcgc gcctgcggtt ggaaggttat
    27061 cgggcggcga tgaaacgtgc gggtctcaac attcctgatg gctatgaagt cactggtgat
    27121 tttgaattta acggcgggtt tgacgccatg cgccaactgt tatcacatcc gctgcgtcct
    27181 caggccgtct ttaccggaaa tgacgctatg gctgttggcg tttaccaggc gttatatcag
    27241 gcagagttac aggttccgca ggatatcgcg gtgattggct atgacgatat cgaactggca
    27301 agctttatga cgccaccatt aaccactatc caccaaccga aagatgaact gggggagctg
    27361 gcgattgatg tactcatcca tcggataacc cagccgaccc ttcagcaaca acgattacaa
    27421 cttactccga ttctgatgga acgcggttcg gcttagattt acgctgtctt ttgatcaaat
    27481 tattaccatc ggttgttttc agaagcatga acattgctgc tgaagcaaca gtaataatgc
    27541 ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt tgtgccttcc atcccttcat
    27601 aaacgcgaag gacggccgca cttacagcaa cgcctaaact aatcgacagt tgctgcgtga
    27661 ccgccagaac actgttaccg ctgctggcgt tgtcatcggt cagatcggca agtgtgatgg
    27721 tattcatcgc ggtaaattgc gtcgacatag ccatccctaa tataaacaac ggcaagatca
    27781 gcatccatat cgccattacc ggtgattgca aagagaactg agcgatcatt agcccaataa
    27841 tcaccgtgat ccccactaac gtatggcgat agcccagacg acgtaagact tgggtaacca
    27901 tcgattttgc aataatggaa cctaacgctg tcggtgccat catacagccg gcaataaacg
    27961 cctgataacc aaatcctacc tgtaacatca atggcataag gaacggtaca caaccggtcc
    28021 ccagacgggt tgcaatattg cctacgatac cgatcgagaa agtgcgggtt ttaaataaat
    28081 ctaatgaaat taatgggttt ggcgtgtgtc gcgcatggag aatatagaga agcagtaacc
    28141 cgatgctggt gacaattacc gtcaaggcaa tccagctggc gacaatcttt tccccgaata
    28201 gctctattcc gcttgagaag agaacaaggc tgaggccaaa cagcaaaaag ccagtgatat
    28261 cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt gcgcgcgtaa agaaggcccg
    28321 caatacctat ggggatattg attaaaaata tccagtgcca ggttgcccag gtgaccagca
    28381 cgccgccaag aacggggcct aaaattggcc ccaccagacc cggcatggcg acaaaattca
    28441 atactggaag aagttcatta cgaggataag cgcgcagtaa ggccagccga gcaacaggca
    28501 tcatcattgc gccgcctatc ccctgaataa cccggaagac aaccagctgt ggtagcgaat
    28561 tagaaagtgc gcaggccaga gaacccaatg tgaacagact cacggcaagg gtaaaaatgc
    28621 gacgcgtacc gaagcgatcg gctagccatc cgcttaccgg aataagcatc gccaccgtca
    28681 gcgtataact gatgatggct gattgcatcg cgagaggaga acgattaagg ctatgagcga
    28741 ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc ctgcatgaag aaggccatcg
    28801 ccgcgatcca cggcaaaccc gccatactgc gcttcttttt atcgctcatt caatgtccta
    28861 ttatcgggtt gtcacttatc aggtgagcgt agcagcgcct gacaagcttt aaatgccgcg
    28921 tcgccatcgc tttggacaat cgcatcgaca atcgcctggt gcagatccag ctttatcact
    28981 gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg aatggaatag cgaggcgaat
    29041 gaggtcaaaa acggattggc gctcatttca tagatatgct catgccaggc catatcgact
    29101 tcgatccagc gttcacggcg aaagttctct tttaatgccg ccatttcggc cattaacgta
    29161 ttgagatgcg ccttctgttc cgcggtgcca accgttgcag ccagtaggca agcttgcggc
    29221 tccagacaga tacgcataac cagaaagtga tcgatgacct gatgaaagtt ctcttctgtc
    29281 atccaccagg taagcaattc ctgatcaaga aaattccagt ttgattgtgg catgacccgg
    29341 gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg ttaacgtttt gaccgcttcg
    29401 cgtaccgctg tacgactcac tccaaattgc tcgcccagct caatctcacc aggcaaaatg
    29461 gtgccgggtt catattcacc ttttaagatg cgttgcgcca gcttctcagc cagaacatac
    29521 gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag gcattactaa tcttcctttt
    29581 tctttttatt cctccttagt atgccaccag gaagtgtgat tacggttgca aaaacggcaa
    29641 attgcttgtt ttatggcaca ttaacggggc ttttgct
//
LOCUS       DAZGJW010000053        29165 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZGJW010000053 DAZGJW010000000
VERSION     DAZGJW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.053"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            415..789
                     /gene="tomB"
                     /locus_tag="ACHKIK_002309"
     CDS             415..789
                     /gene="tomB"
                     /locus_tag="ACHKIK_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO9083472.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            815..1033
                     /gene="hha"
                     /locus_tag="ACHKIK_002310"
     CDS             815..1033
                     /gene="hha"
                     /locus_tag="ACHKIK_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO9083473.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            1205..1756
                     /gene="maa"
                     /locus_tag="ACHKIK_002311"
     CDS             1205..1756
                     /gene="maa"
                     /locus_tag="ACHKIK_002311"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO9083474.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPVRIIKKL"
     gene            1872..2342
                     /gene="ylaC"
                     /locus_tag="ACHKIK_002312"
     CDS             1872..2342
                     /gene="ylaC"
                     /locus_tag="ACHKIK_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO9083475.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            2506..4056
                     /gene="pdeB"
                     /locus_tag="ACHKIK_002313"
     CDS             2506..4056
                     /gene="pdeB"
                     /locus_tag="ACHKIK_002313"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO9083476.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGQLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(4098..4451)
                     /gene="ybaA"
                     /locus_tag="ACHKIK_002314"
     CDS             complement(4098..4451)
                     /gene="ybaA"
                     /locus_tag="ACHKIK_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO9083477.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(4571..4667)
                     /gene="ffs"
                     /locus_tag="ACHKIK_002315"
     ncRNA           complement(4571..4667)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKIK_002315"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4829..5140
                     /locus_tag="ACHKIK_002316"
     CDS             4829..5140
                     /locus_tag="ACHKIK_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO9083478.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(5171..5743)
                     /gene="ybaY"
                     /locus_tag="ACHKIK_002317"
     CDS             complement(5171..5743)
                     /gene="ybaY"
                     /locus_tag="ACHKIK_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO9083479.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            5961..6821
                     /gene="tesB"
                     /locus_tag="ACHKIK_002318"
     CDS             5961..6821
                     /gene="tesB"
                     /locus_tag="ACHKIK_002318"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO9083480.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(6870..8156)
                     /gene="amtB"
                     /locus_tag="ACHKIK_002319"
     CDS             complement(6870..8156)
                     /gene="amtB"
                     /locus_tag="ACHKIK_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO9083481.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(8186..8524)
                     /gene="glnK"
                     /locus_tag="ACHKIK_002320"
     CDS             complement(8186..8524)
                     /gene="glnK"
                     /locus_tag="ACHKIK_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO9083482.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(8705..10486)
                     /gene="mdlB"
                     /locus_tag="ACHKIK_002321"
     CDS             complement(8705..10486)
                     /gene="mdlB"
                     /locus_tag="ACHKIK_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO9083483.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(10479..12251)
                     /gene="mdlA"
                     /locus_tag="ACHKIK_002322"
     CDS             complement(10479..12251)
                     /gene="mdlA"
                     /locus_tag="ACHKIK_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO9083484.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHRALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(12281..12739)
                     /gene="decR"
                     /locus_tag="ACHKIK_002323"
     CDS             complement(12281..12739)
                     /gene="decR"
                     /locus_tag="ACHKIK_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO9083485.1"
                     /translation="MLDKIDHKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(12892..13710)
                     /gene="cof"
                     /locus_tag="ACHKIK_002324"
     CDS             complement(12892..13710)
                     /gene="cof"
                     /locus_tag="ACHKIK_002324"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO9083486.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGRVTKICFCGGHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            13810..15510
                     /gene="ybaE"
                     /locus_tag="ACHKIK_002325"
     CDS             13810..15510
                     /gene="ybaE"
                     /locus_tag="ACHKIK_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO9083487.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKHIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGNSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPYWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15575..16270
                     /gene="queC"
                     /locus_tag="ACHKIK_002326"
     CDS             15575..16270
                     /gene="queC"
                     /locus_tag="ACHKIK_002326"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO9083488.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(16322..16720)
                     /gene="fadM"
                     /locus_tag="ACHKIK_002327"
     CDS             complement(16322..16720)
                     /gene="fadM"
                     /locus_tag="ACHKIK_002327"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO9083489.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(16814..17185)
                     /gene="ybaV"
                     /locus_tag="ACHKIK_002328"
     CDS             complement(16814..17185)
                     /gene="ybaV"
                     /locus_tag="ACHKIK_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO9083490.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(17336..19207)
                     /gene="ppiD"
                     /locus_tag="ACHKIK_002329"
     CDS             complement(17336..19207)
                     /gene="ppiD"
                     /locus_tag="ACHKIK_002329"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO9083491.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKVVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(19399..19689)
                     /gene="hupB"
                     /locus_tag="ACHKIK_002330"
     CDS             complement(19399..19689)
                     /gene="hupB"
                     /locus_tag="ACHKIK_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO9083492.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(19880..22234)
                     /gene="lon"
                     /locus_tag="ACHKIK_002331"
     CDS             complement(19880..22234)
                     /gene="lon"
                     /locus_tag="ACHKIK_002331"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO9083493.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(22422..23696)
                     /gene="clpX"
                     /locus_tag="ACHKIK_002332"
     CDS             complement(22422..23696)
                     /gene="clpX"
                     /locus_tag="ACHKIK_002332"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO9083494.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(23822..24445)
                     /gene="clpP"
                     /locus_tag="ACHKIK_002333"
     CDS             complement(23822..24445)
                     /gene="clpP"
                     /locus_tag="ACHKIK_002333"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO9083495.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(24692..25990)
                     /gene="tig"
                     /locus_tag="ACHKIK_002334"
     CDS             complement(24692..25990)
                     /gene="tig"
                     /locus_tag="ACHKIK_002334"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO9083496.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(26334..26651)
                     /gene="bolA"
                     /locus_tag="ACHKIK_002335"
     CDS             complement(26334..26651)
                     /gene="bolA"
                     /locus_tag="ACHKIK_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO9083497.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            26956..27534
                     /gene="yajG"
                     /locus_tag="ACHKIK_002336"
     CDS             26956..27534
                     /gene="yajG"
                     /locus_tag="ACHKIK_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO9083498.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            27578..29053
                     /gene="ampG"
                     /locus_tag="ACHKIK_002337"
     CDS             27578..29053
                     /gene="ampG"
                     /locus_tag="ACHKIK_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO9083499.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTGVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         6980 a         7853 c         6996 g         7336 t
ORIGIN      
        1 aaaatactga ttgttagtta atttatatta agtagcgcta atagatttaa taatccataa
       61 tcatttagag gctattctta attatttgcg gtaattcttt attcattcct cggttattac
      121 gtcatattca gagcaatcct ggtattagtg tcaccaattt catctggcga taatcctgaa
      181 atgttatgaa cagttcgagc aaactgcttt tacctgctgc gggttagtgc taatatgaaa
      241 aagtgagtcc tgtcccgatt ccttcctaat tgtaattttt cgtaataatg cgatgaaaac
      301 ctgcaaagag tggcttatag ttaagctaac aaacgagagg gcaagtccag gtcagtaagt
      361 tttttccatc ccgaaaggtg tccgttagtt caaccactaa gaaggggacg cgttatggat
      421 gaatactcac ccaaaagaca tgatatcgca cagcttaagt ttctctgtga aaccctgtat
      481 catgactgtc ttgcaaacct tgaagaaagc aatcatggct gggttaacga cccaacctcg
      541 gcgatcaacc tccagttgaa tgaactgatt gagcatattg cgaccttcgc acttaattac
      601 aaaattaagt ataatgaaga caataagctc attgagcaga tcgacgaata tctggatgac
      661 acctttatgt tgttcagcag ttatggtatt aatatgcagg atcttcagaa atggcggaag
      721 tcaggtaatc gactattccg ttgttttgtc aatgcgacga aagagaatcc tgcgagttta
      781 tcttgttaga attattacaa ccatgggtag aagtatgtcc gaaaaacctt taacgaaaac
      841 cgattattta atgcgtttac gtcgttgcca gacaattgac acgctggagc gtgttatcga
      901 gaaaaataaa tacgaattat cagataatga actggcggta ttttactcag ccgcagatca
      961 ccgcctcgcc gaattgacca tgaataaact atacgacaag atcccttcct cagtatggaa
     1021 atttattcgc taataaataa ttcgctttcg gagctataac cggctgttta ttaagaattt
     1081 tatacttttt caccatgaag acatacccta tgtgatcttt atcacacaga tgtaatagga
     1141 acgttctctt cactgacttt tcgtcttact gtgttgccgc attttcagca accggaggtc
     1201 agtaatgagc acagaaaaag aaaagatgat tgctggtgag ttgtatcgct cggcagatga
     1261 gacgttatct cgcgatcgcc tgcgcgctcg tcagcttatt caccgataca atcattccct
     1321 ggcggaagag cacacattac gccagcaaat tctcgctgat ctattcggtc aggtgacaga
     1381 ggcttatatt gagccaacgt ttcgctgtga ctatggctat aacatttttc tcggtaataa
     1441 ttttttcgcc aacttcgatt gcgtgatgct tgatgtctgc cctattcgca tcggtgataa
     1501 ctgtatgttg gcaccaggcg ttcatatcta cacggcaaca catcccatcg accctgtagc
     1561 acgtaatagc ggtgctgaac tggggaaacc cgtcaccatc ggtaataacg tctggattgg
     1621 cggacgcgcg gtcattaacc ctggtgtgac cattggtgat aacgtcgtgg tagcctcagg
     1681 tgcagttgtc acaaaagatg tcccggacaa cgttgtcgtg ggcggtaatc cagtcagaat
     1741 aattaaaaaa ttgtaatcgg tttttcgcaa ctgttatgca aaattgtggt agatctgtta
     1801 cttcccctct actattccca cgttaaaata gggtgttccc tggaaagttg cagataccac
     1861 gaaggcaaac gatgaccgaa atacaacgcc tgctgaccga aacgattgag tctctgaata
     1921 cccgcgaaaa acgcgacaac aaaccccgct ttagtatcag ttttatccgt aaacatccgg
     1981 ggctgtttat cggtatgtac gttgcttttt ttgccaccct ggcggtgatg ttgcagtccg
     2041 aaacgctgtc aggctctgtc tggctactgg ttgtattatt tatcctgctt aatggtttct
     2101 tctttttcga tgtctaccca cgctaccgct atgaagatat cgacgtgctg gatttccgcg
     2161 tttgctataa cggcgaatgg tacaacacgc gctttgtacc tgccgcgctg gttgaagcca
     2221 tcttgaactc tccgcgtgtc gcggatgttc ataaggaaca actgcaaaaa atgatcgtcc
     2281 gtaaaggtga actgtctttt tacgatattt ttaccctcgc tcgcgccgaa tcaacatctt
     2341 aagttagggt tacataccag gcgtaaagct ctgcgcctgg tcaaatgaca atgatcgttt
     2401 ccacccatca cttcatgaaa taccagctct acctccttat ctcctgccag cctttttcca
     2461 caatcagata tactttccct acactgtgtt aataaggata tgctggtgag aacacgacat
     2521 ctggtcggcc ttatttcggg agtactgatt ctttcagtat tgctgcctgt cggcttaagc
     2581 atctggctgg cccatcagca ggtagaaaca tcgtttattg aagagctgga tacctattcc
     2641 tcccgcgtcg ctattcgggc caataaggtg gcgacacaag ggaaagatgc gctgcaagag
     2701 ctggaaagat ggcaaggcgc tgcctgtagc gaagcccatc tcatggaaat gcgtcgggta
     2761 tcttacagtt atcgctatat tcaggaagtg gtttatatcg ataacaacgt tccccagtgt
     2821 tcgtctctgg agcatgaaag tccgcccgat accttccccg agccaggtaa aatttcgaaa
     2881 gatggttatc gtgtctggtt aacatcgcat aacgatttag gcattatccg ttacatggtc
     2941 gccatgggaa cggcacatta tgtcgtcatg atcgaccccg cttcctttat tgatgtcatt
     3001 ccctatagct catggcaaat tgatgccgcc attattggca atgcccataa cgttgtcata
     3061 accagcagcg atgaaattgc tcagggaatt attaccaggc tacaaaaaac acccggtgag
     3121 catatcgaaa ataatggaat catttacgat atcctgccct tcccggagat gaatatttcg
     3181 atcatcacct gggcttcaac gaaaatgttg cagaaaggct ggcatcggca agtctttatt
     3241 tggttaccgc tcgggttggt gattggccag ctggcagcga tgtttgtgct gcgtattttg
     3301 cgccgtatcc agtcaccgca tcatcggctg caggatgcta tcgaaaatcg tgatatttgc
     3361 gtgcactatc agccgattgt ctccttagcc aatggcaaaa ttgtcggtgc tgaggcactg
     3421 gcgcgctggc cgcagacaga cggtagttgg ttgtcaccag atagttttat tccgctggca
     3481 cagcaaacgg gcctttctga gccattgacg ctactgatta taagaagcgt ctttgaagat
     3541 atgggcgact ggctgcgtca gcatccacag cagcatattt cgatcaatct tgaatccacc
     3601 gtgctcacct cggaaaaaat cccgcaattg ctgcgtgaaa tgatcaatca ctatcaggtt
     3661 aatcccagac agatcgcgct tgaactcact gaacgcgagt ttgccgatcc gaaaaccagc
     3721 gccccgataa tttctcgcta ccgggaggcg ggccatgaaa tttatcttga tgattttggt
     3781 acgggatatt caagtttaag ttatttacag gatctggatg tcgacattct gaagatcgat
     3841 aaatctttcg ttgatgcgct ggaatataaa aatgtcacgc cgcatatcat cgaaatggca
     3901 aaaacactga aactgaaaat ggtagcggag ggaatcgaaa ccagtaaaca agaagagtgg
     3961 ctacgccagc atggcgtgca ctacggtcag ggctggctct acagcaaggc attaccgaaa
     4021 gaagatttct tacgctgggc cgagcaacat ttgtgaattt ttgggctgac ggcacgccgc
     4081 acagcccgtc acgctaccta ttcgtcgatg attgactcga atccgccata gatcattcgc
     4141 ttgccgtcaa acggcatgga ctcgccgaac tctttcatcc gtgggtccga catcatcttt
     4201 tgattagcag cgtcgcggac ctcttttgaa gggtattcaa tccagctaaa gacaacctct
     4261 tcattctctt ccgctttcac cgccatacga aaatcggtca ctttgccatc cggtacatcg
     4321 ctggcccagc attcgacaat acgaagcgcg ccaaactctt taaacaatgg cgcagcctta
     4381 gcggccattt cccgataggc atccttttta tcggcaggaa cggcaaccac aaaaccatca
     4441 acatacttca tcagacatac ctccgaagcg tactgcgcag ccagacaatt cttgcgacgt
     4501 cagttgtggt taaagtttag ccatgttggt tttttttgac gaaacggtgg gaggcagaaa
     4561 tgggtggggg ccctgccagc tacatcccgg cacacgcgtc atctgccttg gctgcttcct
     4621 tccggacctg acctggtaaa cagagtagcg ttgcgggaga accaacaggc ccccattgag
     4681 agcgttgaga accaacgcgc aggcgcatta tcactgctgc cccggctaat tgcaacccat
     4741 cgcgtgccag atgctggttt cttgcgcaat gcgacttcac tcgggcgttt ttccagatta
     4801 tgctgaaaaa ttacctcagg aagaaaagat ggaaaaagaa gattcatttc cccaacgcgt
     4861 ctggcaaatc gtcgccgcta ttcccgaagg ctatgtcacc acttacggtg atgtggcgaa
     4921 actggcggga tcgccccgcg ccgcgcgcca ggtgggcggt gtgttaaagc gtctccctga
     4981 aggcagcacc ttaccctggc accgggtggt taatcgccac ggcacaattt cgctaaccgg
     5041 accggattta cagcgtcagc gacaggcatt actggcagaa ggtgtgatgg tatcgggaag
     5101 cgggcaaatc gacttgcagc gttatcgctg gaactactaa ccctctccgg gcggagaggg
     5161 ttaacgtcgc ttaatattgc gtaggagctg gaactgccga agacggattc acctgagttg
     5221 gtgaagtcga aggtacggta gtcgttgcac caccgctggc ctgaaccggc acggcggttt
     5281 gctgcaccgg caccaatgtc aggtcggctt tagttccgcc cttgttgatc accggctgaa
     5341 cggtatcggt aataaatacc agtttgtcat tcacggtaat cgccgcactc aacagaatac
     5401 gcgcgttcgg ctgaacatct gccggattaa atggcagaac aaagctgaat ggtgactgtt
     5461 taccttcggt acgcaccgct ttctgcgcca gcacttttga cggtgcatcg gctaacgacg
     5521 cgtcagaaag tgtcacggtc agcacagcat caggcggcag tgcgactttc tgccggatcc
     5581 agacggtacc ggagacattc ggttgctgga tagcaggttg ttgtgttgct gaaatagatg
     5641 tatttgcagc cggggctggc gtctgaatat ccgcgctttt atctgcgcaa gccgccaacg
     5701 caatcgcaac cgctaaacca ctggccatgt gcacgagttt cattcatttc tccttattat
     5761 caatgcacca gcgggctaac tttcctcgcc ggaagagtgg ttaacaaaat agtaacgtca
     5821 acaagtgtgg cacacatcac gcatttctgc ctgtaattag cccgtaattc agaccattgc
     5881 acccatcgga ccagtagcaa atttgcgtta tactcaactc actttggctt gctgcggcag
     5941 ctttgttact ggagagttat atgagtcagg cgctaaaaaa tttactgaca ttgttaaatc
     6001 tggaaaaaat tgaggaagga ctctttcgcg gccagagtga agatttaggt ttacgccagg
     6061 tgtttggcgg ccaggtcgtg ggtcaggcct tgtatgctgc aaaagagacc gtccctgaag
     6121 agcggctggt acattcgttt cacagctact ttcttcgccc tggcgatagt aagaagccga
     6181 ttatttatga tgtcgaaacg ctgcgtgacg gtaacagctt cagcgcccgc cgggttgctg
     6241 ctattcaaaa cggcaaaccg attttttata tgacggcctc tttccaggca ccagaagcgg
     6301 gtttcgaaca tcaaaaaaca atgccgtccg cgccagcgcc tgatggcctg ccttcggaaa
     6361 cgcaaatcgc ccaatcgctg gcgcacctgc tgccgccagt gcttaaagat aaattcatct
     6421 gcgatcgtcc gctggaagtc cgtccggtgg agtttcataa cccactgaaa ggtcacgtcg
     6481 cagaaccaca tcgtcaggtg tggatccgcg caaatggtag cgtgccggat gacctgcgcg
     6541 ttcatcagta tctgctcggt tacgcttctg atcttaactt cctgccggta gctctacagc
     6601 cgcacggcat cggttttctc gaaccgggga ttcagattgc caccattgac cattccatgt
     6661 ggttccatcg cccgtttaat ttgaatgaat ggctgctgta tagcgtggag agcacctcgg
     6721 cgtccagcgc acgcggcttt gtgcgcggtg agttttatac ccaggacggc gtgctggttg
     6781 cctcgaccgt tcaggaaggg gtgatgcgta atcacaatta aaaaaaatag ccggaggtaa
     6841 aaccgtccgg ctattttttg cagtgcttgt tacgcgttat aggcattctc gccgtgactg
     6901 ttgacatcca gcccttctcg ctcctgctct tccggtacac gcagaccaac cgtcagatcc
     6961 gccagtttgt agccaataaa tgccaccaca ccggaccaga cgatcgtaat ggcgatgctt
     7021 tccagctgta ccagcaactg atggcccatc gtcacacctt cagcgaagcc cacgccaccc
     7081 agcgaactgg cggcaaaaat cccagtcatg atacagccga caatgccaca aacgccgtgc
     7141 acaccgaaga catcgcaggg atcatccacc cgcagcaagc gtttgagcat agtaacgccc
     7201 cacaagcccg ccagaccagc taccacgccg ataatcaacg cgccgccaac cccaatgtag
     7261 ccacacgctg gcgtcacgcc gaccagaccg gcaatcgcgc cagaacacgc tcccagcaga
     7321 gaaggcttac cacgcagcgc ccattcaccg aagatccagc caagaattgc cgccgccgtt
     7381 gcgaccacag tattcacaaa tgccagtgcc gcgatttcat tcgccgtgcc cgctgacccg
     7441 gcgttaaagc caaaccaacc aatatagaga atggcagtac cggtgaagac cattggcagg
     7501 ttgtgcggtt taaacgcctc tttgccgaag cccacgcgtt tgcctatcag atacgcgccc
     7561 accagaccgg cgattgcggc gttaatatgc accaccgtgc cacccgcgaa atccagcgca
     7621 ccgtgagaag ccagcaaacc accgccccac accatatgcg caatcggaat gtaagagagc
     7681 gtcagccata ccaccacgaa aatcaacaca gctgagaagc ggattcgttc cgccagcgcc
     7741 ccaacgatca agccgacggt aatgcaggca aacgagccct gaaacgccac gtggatatac
     7801 tgataaatgc tgcccatcac cgccgtcagt tcgatgtttt ttagcatcaa ccagttaatg
     7861 ttgccgaaga agttgttgcc ctcaccaaac gccagcgagt aaccgtaaac cacccagaga
     7921 atacagacca gtgcaaacgt caccgtcacc tgcgtcagca tcgacagcac gtttttgccg
     7981 cgaatcaacc caccgtaaaa cagggcaatc cccggaatag tcataaacag caccagcgca
     8041 gtacaaatca tcataaacgc attgtcggct ttatcggcca ccgcaggtgc agccattacc
     8101 agtcccggca gcatcgccag tgaagcaagc ccagttttta tcgtcgctat cttcattttt
     8161 cgttcctgtt gctgtgtgcc agagattaca gcgccgcttc gtcggcttcg ccggtacgaa
     8221 tacgaatgac gcgttgcaat tcagcgacga agattttgcc gtcgccaatt tttccggtgt
     8281 aagccgcctt actgacgata tcgatcactt catcgagttg gtcatcagca atcgccacat
     8341 caatttttac ttttggcagg aagttgacgc tgtattccgc cccccggtac agctcggcat
     8401 gccctttctg acgcccgaaa cctttcactt cggtgacggt caggccctga ataccaatgg
     8461 aagataacgc ttcacgaacg tcttccagct tgaatggttt gattatcacg gtcaccagct
     8521 tcatagatcc cctccggtca gaattcggta atggttctgc tacacgaaga aggtattgca
     8581 agcggtgtgc cagaaatgaa aaacgagaaa gagagcagga agttaagaag attgaaaatg
     8641 aaaacgcact atgacagtgc acagtgcgtt acatttttgc accaacgatg aaatattgcg
     8701 ctattcaggc gctcaatgat tcttcttcac gcacgctggc tgccagctct tcgcccgcaa
     8761 gttgcagttg atacatctgc cagtagcgtc cctgggccgc cagcagttgc tggtgagtgc
     8821 cctgctccac ggcttgccca cgatgaagca ccagaatggt gtcggcatca acaatggtcg
     8881 ataagcggtg agcaatcacc accagcgtgg tatgttcacg cacagccgcc agagcatgtt
     8941 gaatcgcctg ttcagtaccg gagtcaatgc tggcggttgc ctcatcaagg atcaggattt
     9001 gcggcgtctc caccaacacg cgtgccagtg ccagcagttg cttttgcccg acggagagat
     9061 tattcccctg ctcgcccagc ggcgtataaa taccatcact catgctgcgc gccagctccg
     9121 ccagttgcac ggtttccagc gcctgccaga cgcgttcttc ggagatatca cgccccagcg
     9181 tcacgttggc gaggaaggta tccgccagca ccaccggatc ttgctgcacc attgccacgc
     9241 cctggcgcag cgcgctgtga cttagcgaac ttaatggacg accatcaagg cgaatctcac
     9301 cttccgttag cgggtaatag cccatcaata aactggcgag ggtgcttttg ccactgccgg
     9361 tatgcccgac cagtgccaca aagttacgcg aaggcacaga gagattaatg ttctttagca
     9421 ccagattgtc atcgcgataa gcaaatgaca cgttatcgac ttcgatggtg ccactctgta
     9481 acgggcgatc atcattgcca tattgctggc gcggtccgtc catcagttca aacacgcgct
     9541 caccagcaac aacagcctgt tgcagcatcg cctgttgcgt ggtcagttcg attaatggtt
     9601 cgttaagtcg cccaagatag ctgataaacg catacagcac gcccacttca atggtgccgc
     9661 tagcggagaa gccaaacagc atcaacaagc cacaaagaat gagcgatgaa aacagactca
     9721 gcagcggacg cagcagaaaa ccgtcgaggc gcagggtttg catcctggcc atatagtgtg
     9781 aacgactggc ctcccccata cgttcgccaa atcgcgcctg ctgacggaac tgctggataa
     9841 cgctcatgcc attgatgatt tcgttaaagc cgtcgttgat atccgctaga taggcgcgca
     9901 cacgacggac aatcggcgtg ctgtaacgct ggtatatcac cattaccacc agcaccaccg
     9961 ggaaaatcat tatcgccacc agcgccattc gccagtcgag gctgaacatc gccaccagca
    10021 tcgcgcccac cagtgcggcg ctacgcagga cagttgccac tacggtaacg tagagatcgc
    10081 ggatcacttc agtgtcatta gtgacgcggg aaatcacctg cccgacgggt tgggtatcaa
    10141 actcgcttaa tggctggcgc aatgcggcat ccatcacgtc ggtacgcaac tgttgcacta
    10201 cgcctactgc cgcccgatta aacagcagcg actgcgcgta atgtagcccg gcggcaaaca
    10261 gttgtagccc aacatacgcc gcagccagcc ctgcaaccat tttcaacggc aggttatttt
    10321 tcgctaccat attgtcgata aaatagctga taagcagcgg cccactgact tctgccgctg
    10381 ccgcaaccca catcatcagg accgcaatcc ccagcggttt acgccacggc gaaccgtacg
    10441 ctaacagacg cttgagagtc ggccacagtt ggctaaaact acgcatcgac ggcctcctcg
    10501 cgaatttccg gagcgtcgtc gagcgccgcc tccagttgtt gatagcgata catatcgcga
    10561 taccagccgc tttgttgcgc cagctcatca tgattgccac gctgggcgat atgtccgtgc
    10621 tgcatcacaa taatttcact ggcttccgtc agtgccgaaa ggcgatgggc actgatgatt
    10681 accgttctac cctgtcccca ctgacgcagg ttatgcagga tctggtgctc tgtgcgtccg
    10741 tccaccgccg aaagcgcatc atcaaggatg aggatttccg cgttgactaa taacgcacga
    10801 gcaatagaga tacgctgttt ttgcccgccg gaaagcatca cgccgcgctc gcccacctct
    10861 gtatcgtaac cttgcggtaa acgcaaaata tcgtcatgta cgctggctaa ccgcgcgaca
    10921 tgctcaatct cttgctgggt ggcattcggg caacccaacg cgatgttgtt cgctacggtg
    10981 tcggaaaaaa ggaacggtgt ctggctaact accgccaggc ggctacgcca gctatcgagt
    11041 tgtaacttcg tcagaggaat atcatgaaag cgaatatccc cctcgctgac gtcgaaatga
    11101 cgctgaatga gcgacaacag ggtacttttg ccggaaccag tcggcccgca gatacccagc
    11161 atctgaccgg gtttcagggc gaaattgacg ttttccagcg cacgatggtc agtctgcgga
    11221 tacgtgaact ggtgaatatt tacatcaagt tcgccacgcc cttccggcac gggttcgcta
    11281 ccatcgttca ccaccggcgc ttccgccagc atcgcacgaa tacggctgta cgcagcacta
    11341 ccacgttcca caatgttaaa catccatgcc agcgccagca ttggccaaat catcagacct
    11401 aaatacatca taaaactggt gagctggccc agcgttaaac tgccctgcac caccatccaa
    11461 ctaccgccgc caatcgccag caagttcgcc ataccaatcg cgatatagat tgtcgggtcg
    11521 aaacgagcat caatacgcgc cacccgcatg ttttttttgc cggtatcttc ggcatccgcg
    11581 gcaaataacg ccgactggcg atcttccaga ccaaaggctt tgatcatgcg gatactggtg
    11641 aggctttcct gggtgcggtc attaagactg gaaaacgccg cctgtgccag cttaaagcgt
    11701 tcatgcaagg catcgccgtt gcgcttaatc atgatcgcca tcactggcat cggtaacagg
    11761 gcaaataagg tcaactgcca gctaatttgc gtagacatca taatcaacac agcgcagccc
    11821 attaccagtg aatccaccag cgtcagcacc ccttctccgg cggcaaacac gacgcgatcg
    11881 acgtcattgg tcgcacgagc catgagatca cccgtgcgat gacgcaggta aaactccggg
    11941 tgctgccggc ttagctgacg gtaataatct tcacgcagtt caacagccag ttggtaagac
    12001 gcgccaaaca gcaatacgcg ccagacataa cgcaggagat aaaccacaac ggcaatcagc
    12061 accatggtgg cgatccacat caggatcagc ccggtagtaa agtgttgttc tgtcacgcca
    12121 tcgacaacaa taccaaccac ttttggcgga accagttgca gcatcgcgat aatgacaagc
    12181 aaggcgacag ccccgagata gcgacgccat tcccgacgga aataccagct taattgagca
    12241 aataatcgca cgcgttgtgt cctgacctga ttctggatat ttattcgatg ggtaaagaag
    12301 tggtgtattt aatctgttcc atcgcgaagc tggaagtgac gtccgacagc cccggcacgc
    12361 tgtttaccag acgcttataa aactcgtcgt agcgtttcat gtcggcaacc tggacgcgca
    12421 tcagataatc gtattcacca gccatgcgcc agaaccccag cacttctggc atttcggtaa
    12481 ccaccgtgac aaagcggcaa taccattcgc tgctgtgatg ttgcgttttt atcagcacaa
    12541 aagcggtcag gccgaggcct attttttccg gatccagcag ggcgactttg ccgataagga
    12601 taccgtcgtc ctccagccgt ttcagacgct tccagcaagg ggtggttgtc agattaacgg
    12661 cttcagccag tgcctgcaaa gagagggtgc aatcctgctg cagtaaggcc agcagcttat
    12721 ggtcaatttt atctaacata gcccttccac agagaatttt tttctctttt aattctattt
    12781 taaaggtgaa atcacgacaa tttattctgt gcggtttaaa caccacggca caaaatgaca
    12841 aattcaacgc aaaaaaaacc gggcaattgc ccggtgctgg aagggatctc gttattcggg
    12901 ggaataaggt agatgtggat agtccagcca gtgcgtcaaa tagtgagaga cagcctgatt
    12961 tcggcaatgt ccaatcaccg gtaaatgcgg gagctccgcg cgcagttgcg gcatcgcatt
    13021 gcccataata aacccgctac cgacgctgcc taacatttcg cgatcgttca tcgcatcacc
    13081 aaaggccatg caatcgcgca acgataaacc taaatgttgg gtcagcaccg tcaatgcagc
    13141 gcctttattg cagcccaccg gcagcacttc gaggcaatcc gtggcggaaa aacacaaatg
    13201 tgcacgctcg cctaatgctt cgtatagctg gatctgcaag cgtgtaagat cgtcgtgacc
    13261 gccacagaag cagatcttgg tgactctgcc gagtggcatt tttttgacat cgattatctg
    13321 ataacgaaaa ccgctataga caaatgcctg caacaacgca gggatctctt tcccggtaaa
    13381 ccaaccgtcg tcattgaaga tatgcatgct ggctcgggta tcccattgct gatacagcac
    13441 cagctccgcg acatccgcag gtaaatcatc acgatgtaaa agttcacctt ccagagaatg
    13501 cacgcgcgtt ccgttgccgg taatcaaata tgcatccagc gatagcgccc cgagaatatg
    13561 ctgcatctcc agcgcatgac gccccgtggc aaaagtgagg gtaatgtcgc gttcacgcag
    13621 tcgcgccaaa gtagagaggg ttttctcacc taaatgatgg tcaggcatca ataaagtgcc
    13681 atccatatca aatgctgcca gacgagccat ttctttctcc actctgtgac acggttaatg
    13741 gtgattgagt atcacctgat atatacggaa gtaatagtga atagttaaat aatattgttc
    13801 cgggtttata tgcgattgct caaccgtctt aaccagtatc aacgtctgtg gcaaccttca
    13861 gccggaaagc cgcaaaccgt caccgtcagc gaactggccg aacgctgttt ttgcagcgaa
    13921 cgccatgttc gtacgctgtt gcgtcaggca caggaggcgg gatggctgga gtggcaggcg
    13981 cagtcaggac gcggaaagcg cggacaatta cgctttctgg tcacgccgga atcgctacgc
    14041 aatgcgatga tggaacaggc actggaaacc ggaaagcagc aagatgtgct ggagctggcg
    14101 caactggccc caggtgagct gcgcactctg ttacagccgt ttatgggcgg acaatggcaa
    14161 aacgatacac ccacgttgcg tattccctac tatcgcccgc tcgaaccgct acaaccaggc
    14221 tttttgcccg gccgtgccga gcagcatctc gccgggcaga tattttccgg cctgacccgc
    14281 ttcgataata atactcagcg cccgattggc gatttagcgc atcactggga aacctctact
    14341 gacgggttac gctgggactt ttatcttcgt tcaaccctac actggcataa cggcgatgca
    14401 gtaaaagcct cacacttaca ccagcgatta ttgatgctgt tacaactgcc agcactggat
    14461 caattattta ttagcgtgaa gcatattgaa gtcacccatc cgcagtgtct gaccttcttt
    14521 ttacatcgcc ccgattactg gcttgcgcac cggctggcga gctattgcag ccatctggcg
    14581 catccgcaat tcccactgat cggcactggt ccttttcgct taacacaatt cacagcggag
    14641 ctggtgcgcc tggagagcca tgattattac catttgcgtc atccgctgct taaagcggtt
    14701 gagtactgga taactccgcc gcttttcgaa aaagatttgg gaaacagttg tcggcatccc
    14761 gtgcaaatca ccatcggcaa accggaggag ctgcaacggg tcagccaggt cagtagtggc
    14821 atcagtttag gtttttgcta tttaacgttg cgcaaaagtc cccgactctc cctctggcag
    14881 gcgcgaaaag tgatctccat tattcatcaa tccggtttat tacaaacgtt agaagtcgga
    14941 gaaaacctga tcaccgccag tcatgcatta ctgccaggct ggactattcc gtactggcaa
    15001 gtaccggatg aagtcaaact accgaaaacc ttgacgctgg tttatcacct accgatagaa
    15061 cttcatacca tggcagaacg cctacaggcg acactggcag cggaaggctg tgaactcaca
    15121 attatttttc ataacgcaaa aaactgggac gacacgaccc tactggcaca cgcagacctc
    15181 atgatgggcg acagattaat tggcgaagca ccggaatata ctctggagca atggctacgt
    15241 tgcgatccac tatggccaca tgttttcgac gctccagcat atgcacatct gcaatcgacg
    15301 ctggacgcgg tgcaagtaat gcctgatgag gaaaaccgat ttaatgccct gaaagcggtt
    15361 tttagccagt taatggcaga cgcgacgctg acgccgctgt tcaactatca ctatcgcatt
    15421 agtgcccctc ccggcgtgaa cggtgtgcga ctgacaccgc gcggctggtt tgaatttacc
    15481 gaagcctggc ttcccgcgcc gtcgcaatga atagctggtc cgggttaatc gcaggcgtta
    15541 ccataacgct tttattgttc aagcaggatt atctatgaaa cgtgctgtcg ttgtgttcag
    15601 tggaggccag gattccacca cctgtctggt gcaggcatta caacaatatg atgaagtcca
    15661 ttgcgtgacg ttcgattacg gtcagcgaca tcgcgcagaa atcgacgtgg cacgcgaact
    15721 ggcgctgaaa ctgggagcac gcgcgcataa ggttcttgat gtaacgctgc tcaacgagct
    15781 ggcggtcagc agcctgacac gtgacagcat tccggtgcct gattatgaac ctgaagccga
    15841 tggcatcccg aatacgtttg tccctgggcg taatattttg ttcctgacgc tggcggcaat
    15901 atatgcgtat caggtaaaag cagaagccgt aattaccggc gtctgtgaaa cggatttctc
    15961 cggctacccg gattgccgcg atgagtttgt gaaagcacta aaccatgccg tcagtttggg
    16021 catggcgaaa gatattcgtt ttgaaacgcc gctgatgtgg attgataaag cggaaacctg
    16081 ggcgctggca gattattacg gcaaactgga tttagtccgt aacgaaacgt tgacctgcta
    16141 taacggcttt aaaggcgacg gttgcggtca ttgtgcggca tgtaatttac gcgccaacgg
    16201 tttgaatcat tatctggccg ataaaccgac ggtgatggca gcgatgaagc agaaaaccgg
    16261 gttgaggtaa ttattccggg tgtcgccgga tgcggcttga gcatccggca ccacaaaacg
    16321 tttacttaac catctgctcc agcttttcgc gcaattcccc ttccagagct aatgctttct
    16381 gcgttttaag atcaatacaa acaaacgtaa taagcgcatc cgctaccacc tgcccttccg
    16441 gctccagtgt aatgacctgg cttaagatgc cgcttttacc gtttaattgc tgcaactgac
    16501 tggtaatagt taacaggtcg cttaataccg ctgggcgacg atagttaata ttgatattga
    16561 ccacgacgaa ggcaatgtta tgggccgtca tccactgaaa actgtcgcta ttttccaacc
    16621 catcccagcg ggcttcttcg agaaattcaa ggtagcgggc gttgttgacg tgctggtaaa
    16681 cgtcgagatg atatccacga actttgattt gtgtttgcat agcgcaataa ccttacgttg
    16741 ttgttataag tacagaggtc ataactggta tgacctcttc agtctggcaa aaaattgcca
    16801 ctatgcaaat taattacagg gttaataccg ccagattacg ttccaccagc gaattgccca
    16861 tccccggcac ctgctttaga tcctccaccg ttttaaacgg accgtactct tcgcgataac
    16921 tgacaatcgc ctgcgctttc ttcaggccaa cgccattcat cgcgcgggct agctcttccg
    16981 cgctggcatt attaatgctg acccgggtgc cttcttcgtc actggctttc gccggtactg
    17041 ctgctttact ttgtgctgca ggagcttccg ctttggtttc taccgccgtc ggtttcgcca
    17101 cagaagctgc cgccagcgcg ctatgagaca ttccggcaca ggccagggac agggtaatga
    17161 gcagtgcttt aattccgtgt ttcatactgt tttctccttg tttgttaaca gtggaatcac
    17221 cttaacggga gtgaaaacag taaacaaatg gcaatgttca gaaatggaaa aggccgcgaa
    17281 agcgaccttt tcttgttaca gctgcaacgc atttttttgc gaggcggctt caggattatt
    17341 gctgttccag cgcatcgcca attttgattt tcgcctcttt acgcaggtta ctcatcagag
    17401 cttcaaagac gatttgtgcg ttgttctggg tgataccctg caccatcgct tttttctgat
    17461 cttccggcat tgaaccttgt ttcacttcat ccagcgccag cagaaccaca ttaccttgca
    17521 tatcggtcgc cataccgtag ctcggtttgt ctttcgctgg cagtggcagt gcaaacgccg
    17581 cctggctaat cgggtcacga ccggaacggc ttaagatttt cggctcgcca aatttcagac
    17641 cggcagcctg catagcttcc gcacctttgc cggctttcaa atcaaccagc agtttctcag
    17701 catccacttt cgcctgttgt tcagctttgt tgtgctgaac cagtgcctta acttgttcct
    17761 gaacatctgc caacggtttc accgcttccg gtttgtgctc gctgatgcgc agcacgaatg
    17821 cgcggtcgcc gtctacggtg atgatgtcag agttgatgcc cggcgcgccg ttttcaccta
    17881 ccagaccgcc gttaaagata gcgtcggcaa ccggcttgaa gttcaactct tccggcaggt
    17941 tatctttgct aaaccaaccc gtctgagtgg ctttaacgcc ggcagcttgc tctgcaccgg
    18001 ccagagactc cgtgtcgttg cttgccgcat cgctcacttt ctgctgcagc gcatagtacg
    18061 catcgagggc tttttcgtgt ttcactttcg ccgcaatgtc gtcacgtact tcgtctaacg
    18121 atttcacttt cgctggctga atgtcgtcca gacgtacaat caggaaaccg accgaagatt
    18181 tgatgacacc agacagttgc cctttttctt tcagaccagc atttttcagt tcgtcgggga
    18241 tagtggcatc ttctaaccaa cccatatcgc cgccgttacg ggcagagata atatcggcag
    18301 atttttcttt ggctaatgca gcaaaatcac cgcctttatt cagctcatca agtaccactt
    18361 tcgcttcatc ttcagttttg gtctggatga tgctgtagcg ggtacgctgc ggctgggtga
    18421 attgatcctg atgctgatcg tagtagctct ggatatccgc atcgctaacc ggttgctgca
    18481 tcgttgcggc atccagcttg atgtaactca cgcggaattg ttccggcgtc atgaaattgt
    18541 ttttgttttg ttcgtagtag ctggcaatct cctgttcggt cacaggctgc ttcgccgcca
    18601 gcgcgttaac atcgatagtc gcctcacgca ccacgcgttg ttgcgcgacc agtgccgcca
    18661 gctcgtcggt ttcacctttc agcataaaat cggtaccggc aacaccgtta atcagctgtt
    18721 gggtagtgag ctggttacgc agcgcctggg cgtactgatc ggcggtcatc cccatctggt
    18781 tgaggatacc gttatagcgg ctgttatcaa atttgccgtc aacctggaag gctggggtcg
    18841 cgaaaatcgc ctgtttaacc tgctcatcac tgatacccag tttcagctca cgagcgtact
    18901 gatccaacag cgcctcgtcg atcagacgat tcagcacctg ttgacgcagg gttttcatat
    18961 agccttcgtt cgctgccagc tcggagtatt gatcgcccag ctgttgctgc atgcgattac
    19021 gctcgctgtt aaaggcgttc tcgaactgcc cacggctgat ttcctggtca ttcacttttg
    19081 cggcgtagtt attgcctccg ccaatcaggt aaccactcac gccggtcaat atgaacgaca
    19141 cgataatgat accgaaaata atcttgagca cgagactgtt tgcagccgtg cgtaagctgt
    19201 ccatcatggt gtaacaacac tccgctgtag atgactgtgt acctcacgca acgtatcgat
    19261 gcgcgcaaag gcctattgtg acaagaaacg ggggcaattg tcagcccaca agacacagaa
    19321 agtcaccgaa tacaaataaa aaaggcacat cagtagatgc gcccttgaac ttcgtcacat
    19381 ccccactggg gacaacgctt agtttaccgc gtctttcagt gctttacctg cacggaagct
    19441 cggtacttta gcagcagcga tggtgatctc tttaccggtc tgcgggttgc ggccagtacg
    19501 ggcagcacgc tctttaacgg caaaagtacc aaaacctacc agtgctacat catccccttc
    19561 tttcagagat tcagttacgg aagcaataat agcatctaac gcacggccag ccgcagcttt
    19621 agagatatca gcccctgcag caatcttgtc gatcaattga gatttattca ctcttctctt
    19681 cctctttata atttatatcg cacttgaatc cttcaaggta cgaacgcgca gcagttatat
    19741 caggccagcc atccccttac aacacccctt aataagggca agcccgatcc gccatctaac
    19801 ttagcgagac aaaaaaaggc tggcaagccc gaattagcct gccagccctg tttttattag
    19861 tgcattttgc gcgaggtcac tattttgcag tcacaacctg catgccagac ggttcatttt
    19921 gcagcgccag agtcagaact tcctcaatgc gcttcacagg atgaatgtcc agatcggcaa
    19981 ttacgttgtc aggaatctct tccagatcgc gtttattttc gaacggaatt agcactgttt
    20041 taatcccgcc gcgatgcgct gccaggagtt tttctttcaa accaccgatc ggcagtacct
    20101 gaccacgcag agtgatctca ccggtcattg ccacatcggc acgaaccggg ttaccggtca
    20161 ggcaagaaac cagcgcggtg cacatagcaa taccggcact cggaccatct ttcggcgtcg
    20221 caccttccgg tacgtggacg tggatgtcac gtttttcgta aaaatcaggg ttgatcccca
    20281 gtttttccgc acgcgcacga accaccgtta acgccgcctg aatagactcc tgcatcactt
    20341 cgccgagcga accggtatag gtcagtttgc ctttacccgg aacacatgcg gtttcaatgg
    20401 tcagcaagtc accgcccact tccgtccacg ccagaccggt tacctgaccg acacggtttt
    20461 cgttatcagc gcgaccatag tcgaaacgct gaacaccgag gtagtcatgc aggttatcgc
    20521 cgttaatttc gatatgtttt aatgacttat cgagcagtaa ctgcttaacc gctttgcggc
    20581 acagtttgga gatttcacgc tccagaccac gcacgcccgc ctcacgggtg tagtaacgaa
    20641 taatgccgat aatggcgcta tcgtcgacgg tcagctcacc ttttttcagt gcattacgtt
    20701 caatctgctt cggcagcagg tgacgtttgg cgatgttcag tttttcatct tcggtataac
    20761 cggagaggcg aatcacttcc atacgatcca gcagcggtgc cggaatgttc atggagttcg
    20821 acgtcgcgac aaacatcacg tcgctgagat cgtaatccac ttccaggtag tggtcgctga
    20881 acgctacgtt ctgctctgga tccagcactt caagcagtgc agaggccgga tcgcctcgca
    20941 tgtcagaaga cattttgtcg atctcatcga gcaggaacag cgggtttttc acgcccactt
    21001 tcgccatttt ctggatcagt ttacccggca tagaaccgat gtaagtacgg cggtgaccac
    21061 ggatttccgc ttcatcacgc acgccgccca gcgccatacg gacatattta cgcccggtgg
    21121 ctttggcaat ggactgacca agagaggttt tacctacccc cggcggccct accaggcaga
    21181 ggatcggtcc cttgattttg ttgacacggc tttgaaccgc aaggtactca aggatgcgat
    21241 ctttcacgcg ctccagacca taatggtcgg tatcaaggat ttcctgcgcc tgacgcaggt
    21301 cttttttgac cttgctgcgc gcattccacg gtacctgtac catccagtcg atataaccac
    21361 gcactacggt cgcttctgcc gacatcggag acatcatttt cagcttctgc aactctgctt
    21421 ccgctttctc ttttgcctct ttcggcattt tcgccgcgtc gattttgcgc ttcagggctt
    21481 cgttttcgtc cggcgcgtcg tccatttcac cgagttcttt ctgaatagct ttcatttgct
    21541 cgttcagata gtactcacgc tgggatttct ccatctgctt tttaacgcgg ttgcgaatgc
    21601 gtttctcaac ctgcagcaga tcgatttccg attccatcat tgccatcaga tattccagac
    21661 gttcgttaac gtcggacatc tccagaacgg actgtttgtc agccagtttc agcggcatat
    21721 gtgcagcaat ggtatccgcc agacgcgccg gatcgtcgat gctattcagc gacgtcagca
    21781 cttctggtgg gatttttttg ttcagcttga tgtagccttc gaactggctg attgcagtac
    21841 gcaccagcac ttcctgttcc cgctcatcaa tggtcggcga ctccagatac tccgccttcg
    21901 cagaaaagtg ttcgccattg tcagagagcg cagaaatacg cgcgcgctgt aacccctcga
    21961 ccagcacttt gacggtgccg tcaggcagtt tcagcatctg caatatagag gccacggtcc
    22021 cgacggtgaa aagatcgttt acacccggct catccgttga agcttctttc tgcgcgacca
    22081 gcataatttt tttatcatgg tccatcgccg cttccagaca acggatagat ttttcccgcc
    22141 cgacaaataa ggggatgacc atgtgcggat aaaccaccac atcgcgcagc ggcaatacgg
    22201 ggatttcaat gcgttcagaa cgctcaggat tcatagagct ctctcttagt ttaatttccg
    22261 ccaggtaatc agatgacacg actgtgcttc acgccatcta ttaacatgta cgtcagtata
    22321 tggggatgtt tcccccacat tcaacgccga gaatagagga aaaattaaag gggagataaa
    22381 atcccccctt tttggttaac taattgtatg ggaatggtta attattcacc agatgcctgt
    22441 tgcgcttccg gcttaccata aatcagcaac ggtttgcttt gaccatcaat taccgactcg
    22501 tcgataacca ctttttcgac atcttccatg gacggcagat cgtacatggt atcgagcagt
    22561 gcggcttcta cgatggaacg caggccacgg gcaccggttt tacgcgccat cgctttctta
    22621 gcgatagcat ccagcgcctc gtcacggaat tccagatcca cgccttccag attaaacagc
    22681 gcctgatact gcttggtcag ggcgtttttc ggctctttga ggatctgaat cagagcttct
    22741 tcgctcagtt cattcaacgt tgcgacaacc ggcagacgac caataaactc agggataaga
    22801 ccaaacttga tcagatcttc cggttcaacc tgcgccagca gctcgccttc gcttgctttg
    22861 tcggacttcg cttttaccgt cgcgccaaaa ccaatgccgg agccggtttc tacacggtgg
    22921 gaaatcactt tatccagacc ggcaaacgca ccgccacaaa taaacaggat cttagaggta
    22981 tcaacctgca agaattcctg ctgcggatgt ttacgcccac cttgcggtgg aacagcagct
    23041 accgtacctt cgatcagttt caacagtgcc tgctgtacgc cttcaccgga aacgtctcgg
    23101 gtaatggacg ggttgtctga cttacgagaa atcttgtcga tctcatcgat gtagacaatc
    23161 ccgcgctgcg ctttctgtac gtcgtaatcg cacttctgca acagcttctg aatgatgttt
    23221 tcaacgtctt cgcccacata accggcttcg gtcagcgtgg ttgcgtcggc catggtgaac
    23281 gggacgtcca gcaggcgcgc cagcgtttca gccagcagcg ttttaccgga accggtcgga
    23341 ccgatcagca gaatgttact tttgcccaac tcgacaccat tgctggtatc gccgttgcgc
    23401 agacgtttgt aatggttgta taccgcgacc gccagcactt ttttcgcctg ttcctggccg
    23461 ataacgtaat cgtccaggtg gttgcgaatt tcatgcggcg tcggtagcgc actgcgttca
    23521 cgatgcggtg caacttcttt aatctcttcg cgaatgatgt cgttacataa atcaacacat
    23581 tcgtcgcaga tatacacgga tggaccggca atcagcttgc gcacttcatg ctggcttttg
    23641 ccgcaaaaag agcaatacag caatttgcct gagccatctt tgcgtttatc tgtcatgggt
    23701 caaaacctct tctttgttct ttgtgccgca cacgacgacg caaatgccat tctcaggcgc
    23761 aagccgctta cagcgttgtg ccgccctgga taagtatagc ggcacagttg cgcctctggc
    23821 atcaattacg atgggtcaga atcgaatcga ccagaccgta ttccaccgct tcaggggcgg
    23881 aaaggaagcg atcgcgctcg gtatcacgtt caatctgttc taatgattga cccgtatgaa
    23941 gcgccataag ttcattcatg cgccctttaa ctttcagaat ttcacgggca tgaatttcga
    24001 tatcggtcgc ctggccctgg tagccgccca acggttggtg aatcatcacg cgcgaattcg
    24061 gcaggcaaaa acgtttacct tttgcccctg cggtcaacaa gaaagcgccc atcgaggccg
    24121 cctggcccat acagatggtg ctgacatcag gcttgataaa ctgcatggtg tcatagatag
    24181 acatcccggc agtgatcacc ccgcctgggg agttaatgta cagatagata tctttttctg
    24241 ggttttccgc ttccaggaac agcatctgcg ccacaatcag gttagccatg tggtcttcaa
    24301 cctggccagt cagaaaaatg acgcgttcct taagtagacg agaatagata tcaaaagagc
    24361 gctcaccgcg tgaggtctgt tcaatgacca tcggcaccag cgccatatgg ggtgcaaagt
    24421 tatctcgttc gccgctgtat gacatttccg tctcctggat aaaattgaaa aaacctgctg
    24481 taccgattgt aaccttatgg acggattatc agctagtccc tgtgttatgg gtacggcatc
    24541 accatatttc aagcataaca atcttttgtt gttacgctaa caccgaaaga ggcttttcgc
    24601 acggttccat gcaaaattca tgacaaaaaa aaagcccacc acctggcggt gacgggcctt
    24661 tgtgcgaatt tagcgcgtta tgctgcgtaa attacgcctg ctggttcatc agctcgttga
    24721 aagtggtttc tttttcagtc actttcgctt tcgccagtac agcttcaaca gcctgttctt
    24781 ccagagcaac attgcgcatg ttgtccatca gttctttgtt tttgctgtag aactcgataa
    24841 cttctttcgg atcttcgtac gcagaagcca tctcttcgat caggcctttc acgcgctctt
    24901 cgtcagcttt cagctcgttg gtgcggataa cttcgcccag cagcaggcca acaactacgc
    24961 ggcgtttagc ctgttcttcg aacagttcgc gcggcagttc cagagcttgt ttttcgttgc
    25021 caccgaaacg ctgtgcagcc tggcgacgca gaacgtcgat ttcgctgtcg atcagcgcag
    25081 ccggtacgtc gatgtcgtta gcttttacca gaccttcgat cgcctgagac ttaacgcggt
    25141 tacggatggc gctcttcagc tcgcgctcca tgtttttacg cacttcagcg cgcagacctt
    25201 ctacggaacc atcttcaacg ccgaaacgtt tgatgaattc tgcagtcagt tccggcagtt
    25261 cacgctcttc aactttcttc aggttgatag cgaatttcgc tgctttacct ttcaggtttt
    25321 ctgcgtggta ttcttccggg aaggtcacgt cgatggtgaa ctcttcgcca gctttgtggc
    25381 ctttgatacc gtcttcaaag cccgggatca tacgaccctg gcccatcgcc agtacgaaat
    25441 cagacgcttt accgccttcg aactcttcgc cgtctacaga accggtgaag tcgatggtta
    25501 cgcggtcttc tgcttcaaca gcgccgtctt tttctttcca ggtcgcctgc tgtttacgca
    25561 gagtatccag catgccgtca acgtcagcgt cggtcacttc aacgatcggt ttttcaactt
    25621 cgatcgcttc cagaccctgc agttcaactt ccggataaac ttcaaactct acagagtaag
    25681 tgaagtcttc acccagcttg tattcgcccg gaacataagt cggtgcgcca gccggattga
    25741 ttttttcttt aatgatggcg tcaatgaagt tacggctcat caggtcaccc agaacgtcct
    25801 ggcgtacaga cgcgccataa cgctgagcaa cgatattcat tggcactttg cctttgcgga
    25861 agccgtcaat acgtactttt ttcgcaacgt tgaccagctc gcttttaaca gcggtctcga
    25921 tgctgtcagc agcgatagta atcgttacac ggcggccaag gccttgagtg gtttcaactg
    25981 aaacttgcat cttgttacct caaaaaatca cagtgctcgg tcaactctta ccccgcaagc
    26041 acaggttgtt ggcttcgcgg aaccgggatg ttcttataat caatcacatt ccctgttacc
    26101 agaatcatcc cgaaagcatt caaataggac gcggcattat agcggcatca cttttatgag
    26161 tcgagaacgg ttattgcgcg ctgtcgcgta ttttttcaca attcccgggc aattttcgtc
    26221 tgaatgctgg gcaaaaagcg caaaatccag acaaaacaaa acggcccgca ggccgttttt
    26281 cataaaatct ttagcaacat actggaaaag cgccgacagt tgcaaatgcg tttttacgcg
    26341 atgcttcctg ctccacgaca gggaggagag gcaaagacgg tgtcctgcaa cccttcccac
    26401 tccttaatag tgtaagtatg cagagccagc gcatgaacgg tagtagagag ttcctccgct
    26461 aaagtactgt aaatcattcg atgacgattc agaaaacgtt cacccgtaaa acgatcgctg
    26521 accagcacaa ctttaaaatg gctttcagag ccggctggga cattgtgacg atagctttca
    26581 tccactactt cgaggaatac gggttggaac gccgccctta atttttcttc tatccgctca
    26641 cgtatcatca tgaaatatct cctccgacaa cgctgagatg tcatccatcc ctttaaatac
    26701 tagccgcttt taccgtttcc attgcagctt aacaacaaat atttaggttt cgtcctggtt
    26761 tttccgtcaa gcgtatgaag tttactgatt taaagattga aacacaggcc tcgcatcagc
    26821 gtttctttta ccaggggtga ttatttcgtc aacatgatga atttacatgc cttacccact
    26881 tcccctcgcc gttggcaatg ttatgatggc gggaattttt accaaacacg cttaagagtc
    26941 tctgagagcc cgaacatgtt caaaaaaatc ctcttcccgt tagttgctct gtttatgctt
    27001 gcaggatgcg caaaaccgcc aacaactatt gaagtttccc cgacgattac actgccacag
    27061 caggatccaa gcctgatggg cgtcaccgta agcattaatg gtgccgatca gcgtaccgat
    27121 caggcgctgg caaaagtcac ccgcgataat caaatcgtta ccctgaccgc ctcccgcgat
    27181 ctgcgtttcc tgctgcaaga agtgctggaa aaacagatga ccgcgcgtgg ttacatggtt
    27241 ggtccgaatg gcccggttaa tctgcaaatc atcgttagcc aactgtatgc tgacgtgtcc
    27301 cagggcaacg tgcgctacaa catcgctacc aaagcggata tcgccatcat cgctaccgcg
    27361 cagaatggca acaaaatgac caaaaactat cgtgccagct acaatgttga aggtgcgttc
    27421 caggcctcca acaaaaatat cgccgatgcg gttaacagtg tgctgaccga taccatcgct
    27481 gatatgtctc aggacaccag cattcacgaa ttcatcaagc agaacgcgcg ttaatttctg
    27541 ccctctggcc cggtgcaagc cgggcctgta gacgcccatg tccagtcaat atttacgtat
    27601 ttttcaacag ccgcgttcag ccatattgct gatcctgggt tttgcttccg ggctaccgct
    27661 cgccctgaca tccggcacct tacaggcctg gatgacggtc gagaatattg atctcaaaac
    27721 cattggtttc ttctctctgg taggccaggc ttacgttttt aaattcctct ggtcaccgct
    27781 gatggaccgc tacacgcccc cattttttgg gcggcggcgc ggttggctgc tcgccacgca
    27841 aatcctgtta ttagtcgcca ttgcggcgat gggttttctc gaacctggca cccaactccg
    27901 ctggatggcg gcactggcgg tggtgatcgc tttttgctcg gcctcccagg atattgtctt
    27961 cgatgcgtgg aaaaccgatg tgctcccggc agaagaacgt ggtgcgggcg cggcaatcag
    28021 cgtgctgggt taccgtttag ggatgctggt ttccggcggc ttagccctgt ggctggcaga
    28081 taaatggctg ggctggcagg gcatgtactg gttgatggcg gcgctgttga tcccttgtat
    28141 tatcgcgacg ttgcttgcac cagaaccgac cgacaccatt cctgtgccaa aaacgctgga
    28201 acaagcggtt gttgcacctc tgcgagattt ctttggtcgc aataatgcct ggcttatttt
    28261 gcttcttatc gtgctgtata agctgggcga cgcattcgcc atgagcctga caaccacgtt
    28321 tttgattcgc ggcgtcgggt ttgatgcggg tgaagtaggc gtggttaaca aaacgcttgg
    28381 cttactggcg accattgtgg gcgcattgta cggtgggatt ttgatgcagc gcctgtcgct
    28441 gttccgggcg ctgctgattt tcggcatttt acaaggtgcg tctaacgctg gttactggct
    28501 gctgtcgatt actgataagc atctctacag catgggcgca gccgtctttt tcgaaaacct
    28561 ctgtggcggg atgggcacat cagcctttgt cgcgctgtta atgacgctgt gtaataagtc
    28621 attttccgca actcaatttg ccctgctctc agcgctttcc gctgtaggac gagtttatgt
    28681 cggccccgtg gcgggttggt ttgttgaagc acacggctgg tcgacattct atctattctc
    28741 cgtcgccgct gccgtaccgg ggcttatttt gctgctggtt tgccgccaga cgcttgaata
    28801 tacaggagta aatgacaact ttatctcccg taccgaatat ccggcaggtt atgcctttgc
    28861 catgtggaca ctggcggcgg gcgtcagcct gttggccgtg tggttactgc tgttgacgat
    28921 ggacgcgctg gatttgacgc acttctcttt cctgcctgct ctgctggaag tcggggtttt
    28981 agtcgccctt tctggcgtcg tgcttggtgg tttgctggat tatctggcgc tacgaaaaac
    29041 gcatctgacg taatctgtaa atattattta gctgtagtaa tcactcgccg taattattac
    29101 ggctaaataa acatcagtat cgcttaataa tatttgttct tttgtgcggc ttagcgtttg
    29161 gaaat
//
LOCUS       DAZGJW010000054        28650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZGJW010000054 DAZGJW010000000
VERSION     DAZGJW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.054"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..596)
                     /locus_tag="ACHKIK_002338"
     CDS             complement(<1..596)
                     /locus_tag="ACHKIK_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO9083500.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(667..2781)
                     /gene="fusA"
                     /locus_tag="ACHKIK_002339"
     CDS             complement(667..2781)
                     /gene="fusA"
                     /locus_tag="ACHKIK_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO9083501.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="ACHKIK_002340"
     CDS             complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="ACHKIK_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO9083502.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="ACHKIK_002341"
     CDS             complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="ACHKIK_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO9083503.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="ACHKIK_002342"
     CDS             complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="ACHKIK_002342"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO9083504.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="ACHKIK_002343"
     CDS             complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="ACHKIK_002343"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO9083505.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="ACHKIK_002344"
     CDS             complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="ACHKIK_002344"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO9083506.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4985..5707)
                     /gene="yheO"
                     /locus_tag="ACHKIK_002345"
     CDS             complement(4985..5707)
                     /gene="yheO"
                     /locus_tag="ACHKIK_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9083507.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="ACHKIK_002346"
     CDS             complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="ACHKIK_002346"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO9083508.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6907..7125
                     /gene="slyX"
                     /locus_tag="ACHKIK_002347"
     CDS             6907..7125
                     /gene="slyX"
                     /locus_tag="ACHKIK_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO9083509.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="ACHKIK_002348"
     CDS             complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="ACHKIK_002348"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO9083510.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7859..8059)
                     /gene="yheV"
                     /locus_tag="ACHKIK_002349"
     CDS             complement(7859..8059)
                     /gene="yheV"
                     /locus_tag="ACHKIK_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO9083511.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="ACHKIK_002350"
     CDS             complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="ACHKIK_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO9083512.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="ACHKIK_002351"
     CDS             complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="ACHKIK_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO9083513.1"
                     /translation="MSQPAKVLLLFAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10556..12469
                     /gene="yheS"
                     /locus_tag="ACHKIK_002352"
     CDS             10556..12469
                     /gene="yheS"
                     /locus_tag="ACHKIK_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9083514.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12469..13491
                     /gene="yheT"
                     /locus_tag="ACHKIK_002353"
     CDS             12469..13491
                     /gene="yheT"
                     /locus_tag="ACHKIK_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO9083515.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13485..13703
                     /gene="yheU"
                     /locus_tag="ACHKIK_002354"
     CDS             13485..13703
                     /gene="yheU"
                     /locus_tag="ACHKIK_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO9083516.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13757..14626
                     /gene="prkB"
                     /locus_tag="ACHKIK_002355"
     CDS             13757..14626
                     /gene="prkB"
                     /locus_tag="ACHKIK_002355"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO9083517.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14681..15085)
                     /gene="yhfA"
                     /locus_tag="ACHKIK_002356"
     CDS             complement(14681..15085)
                     /gene="yhfA"
                     /locus_tag="ACHKIK_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO9083518.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15387..16019
                     /gene="crp"
                     /locus_tag="ACHKIK_002357"
     CDS             15387..16019
                     /gene="crp"
                     /locus_tag="ACHKIK_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO9083519.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16070..18160
                     /gene="yhfK"
                     /locus_tag="ACHKIK_002358"
     CDS             16070..18160
                     /gene="yhfK"
                     /locus_tag="ACHKIK_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO9083520.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18227..19447)
                     /gene="argD"
                     /locus_tag="ACHKIK_002359"
                     /gene_synonym="dapC"
     CDS             complement(18227..19447)
                     /gene="argD"
                     /locus_tag="ACHKIK_002359"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO9083521.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="ACHKIK_002360"
     CDS             complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="ACHKIK_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO9083522.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRYRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20128..20730)
                     /gene="fic"
                     /locus_tag="ACHKIK_002361"
     CDS             complement(20128..20730)
                     /gene="fic"
                     /locus_tag="ACHKIK_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO9083523.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20720..20887)
                     /gene="yhfG"
                     /locus_tag="ACHKIK_002362"
     CDS             complement(20720..20887)
                     /gene="yhfG"
                     /locus_tag="ACHKIK_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO9083524.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="ACHKIK_002363"
                     /gene_synonym="rotA"
     CDS             complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="ACHKIK_002363"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO9083525.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21835..23016
                     /gene="tsgA"
                     /locus_tag="ACHKIK_002364"
     CDS             21835..23016
                     /gene="tsgA"
                     /locus_tag="ACHKIK_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO9083526.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23278..25821
                     /gene="nirB"
                     /locus_tag="ACHKIK_002365"
     CDS             23278..25821
                     /gene="nirB"
                     /locus_tag="ACHKIK_002365"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO9083527.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25818..26144
                     /gene="nirD"
                     /locus_tag="ACHKIK_002366"
     CDS             25818..26144
                     /gene="nirD"
                     /locus_tag="ACHKIK_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO9083528.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26270..27076
                     /gene="nirC"
                     /locus_tag="ACHKIK_002367"
     CDS             26270..27076
                     /gene="nirC"
                     /locus_tag="ACHKIK_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO9083529.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLSNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27095..28468
                     /gene="cysG"
                     /locus_tag="ACHKIK_002368"
     CDS             27095..28468
                     /gene="cysG"
                     /locus_tag="ACHKIK_002368"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO9083530.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6912 a         7711 c         7245 g         6782 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcgtactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcatat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cacgagcagc accgccgtag
      481 gttttagcca gtacggtggt gattgcagcg gtcagagtag ttttaccgtg gtcaacgtgg
      541 ccgatagtac caacgttaac gtgcggtttt gtacgttcaa atttttcttt agacacggct
      601 atattcctta ctatagtgct ctccccttca ggagagagca cgggactttg gtattaaccc
      661 ttaggcttat ttaccacggg cttcaattac ggcctgagca acgttactcg gcgcttcatc
      721 atacttcagg aattccatag tgtatgatgc acgacctttg gtcagagaac gcagctgagt
      781 tgcgtatccg aacatttcag acagcggtac ttcagcgtgg atcttaacgc cagtaacttc
      841 agattcctga cctttgagca taccacgacg acggctcaag tcaccgataa cgtcaccggt
      901 gttctcttcc ggagtttcta cttcaacctt catgatcggc tcaagcagaa ctggtttcgc
      961 tttcttaaag ccttctttaa aggcgataga agcagccagt ttaaacgcca gttcagagga
     1021 gtcaacgtca tggtaagaac cgaagtgcag acgaataccc atgtctacta ccgggtagcc
     1081 tgccagcgga cctgctttca gctgttcctg gataccttta tcaacggccg ggatgtattc
     1141 gccagggatt acaccacctt taatgtcgtt gatgaactcg tagcctttcg ggtttgaacc
     1201 cggctccagc gggtacatgt cgataacaac atgaccatac tgaccacgac caccagactg
     1261 tttcgcgtgt ttaccttcaa catcggtaac tttctggcgg atagtttcac ggtaagcaac
     1321 ctgcggttta cctacgttcg cttcaacgtt gaattcacgc ttcatacggt caacgatgat
     1381 gtcgaggtgc agttcgccca tacccgcgat aatggtctgg ttagattctt cgtcagtcca
     1441 tacacggaaa gacgggtctt ctttagccag acggcccaga gccagaccca ttttttcctg
     1501 gtcagctttg gttttcggtt caactgcgat ggagattacc ggctcaggga attccatacg
     1561 ttccagaatg atcggcgcat ccgggtcaca cagggtgtca ccagtggtta cgtctttcag
     1621 accgatagca gcagcgatgt cgcccgcgcg aacttctttg atctcttcac gtttgttagc
     1681 gtgcatctga acgatacgac cgaaacgctc acgtgcagct ttcacggagt tcagtacggt
     1741 atcaccagag ttaaccacac cggagtaaac acggaagaag gtcaggttac caacaaacgg
     1801 gtcggtagcg attttgaacg ccagtgcaga aaacggctcg tcatcacttg cgtgacgctc
     1861 agccggagtg tctttaccgt cgtccaggat accgttgatc gcaggtacgt caaccgggga
     1921 tggcaggtaa tcaattaccg catccagcat cgcctgaaca cctttgttct tgaacgcaga
     1981 accacaggtt accaggatga tttcgttgtt cagaacgcgc tgacgcagag cacctttgat
     2041 ttctgcttca gtcagttctt caccacccag gtatttttcc atcagctctt cagaagcttc
     2101 agctgcggat tcgatcaggt tctggtgcca ttcgttagcc agttcaacca tgtctgccgg
     2161 gatatcttcg tattcgaagg ttacgccctg gtcagcgtcg ttccagttga tagctttcat
     2221 tttcaccagg tcaacaacac cggtgaaatg ttcttcagca ccaatcgcca gctgcagcgg
     2281 aaccgggttc gcgcccagac gggttttgat ctggttaaca actttcagga agttcgcacc
     2341 catgcggtcc attttgttaa cgaacgcaat gcgcggaact ttatatttgt ttgcctgacg
     2401 ccatacggtt tcagactgcg gctgaacacc accaactgcg cagtaaacca ttaccgcacc
     2461 atcgagaaca cgcatggaac gttctacttc gattgtgaag tcaacgtgcc ccggggtgtc
     2521 gatgatgttg atgcgatgcg gctcatactg cttagccata ccagaccaga atgcagtagt
     2581 cgcagcggaa gtgatggtaa taccacgttc ctgctcctgc tccatccagt ccatggttgc
     2641 agcgccgtca tgaacttcac cgattttatg gtttacaccg gtgtagaaca gaatacgttc
     2701 ggtagtagtg gttttaccgg cgtcgatgtg cgcactgata ccgatattac ggtagcgtgc
     2761 gatgggtgtt gtacgagcca tttgtttcct cgtttatctt ttaggcgttc aatttaagta
     2821 gcccaaagcg ggctgcttac tggaagcgcc cgcctggtga ctaaaactcc gaagggatta
     2881 ccaacggtag tgtgcgaacg ccttgttggc ttcggccata cggtgaacgt cttcacgttt
     2941 cttaactgca gtacctttgt tttctgcagc atcagaaagt tcgttcgcca ggcgcagagc
     3001 catggattta tcaccgcgtt tacgagcagc ttcaacgatc caacgcattg ccagagcatt
     3061 acgacgaacc ggacggactt caactggtac ctgataagta gaaccaccaa cgcggcgaga
     3121 cttaacttct acagtcgggc gcacgttttc gagagctact tcgaatgctt ccagttcaga
     3181 tttaccagag cgctgagcca gggtctccag cgcgctgtat acgatagatt cagcagtaga
     3241 ttttttacca tctaccatca ggatatttac aaatttagcc agcagttctg atccgaactt
     3301 cggatccggc agaattttac gctgaccaat gacgcgacga cgtggcatgg aaatactccg
     3361 ttgttaattc aggattgtcc aaaactctac gagtttagtt tgacatttaa gttaaaacgt
     3421 ttggccttac ttaacggaga accattaagc cttaggacgc ttcacgccat acttggaacg
     3481 agcctgctta cggtctttaa cgccggagca gtcaagcgca ccacgtacgg tgtggtaacg
     3541 aacacccggg aggtctttaa cacgaccgcc acggatcagg atcacggagt gctcctgcag
     3601 gttgtgacct tcaccaccga tgtaggaagt cacttcgaaa ccgttagtca gacgaacacg
     3661 gcatacttta cgcagcgcgg agttcggttt tttaggagtg gtagtatata cacgagtaca
     3721 tacgccacgt ttttgcgggc atgcttccag cgcaggcacg ttgcttttcg caactttgcg
     3781 agcacgtggt ttgcgtacca gctggttaac tgttgccatt aaatagctcc tggttttagc
     3841 ttttgcttcg taaacacgta ataaaacgtc ctcacacaat atgaggacgc cgaattttag
     3901 ggcgatgccg aaaaggtgtc aagaaatata caacgatccc gccatcacca ggccatctgg
     3961 ctggagtgct taaccgtaag tctgacgaaa tcagtatagt caatgggaat gatgtcgttc
     4021 gaaatttgac cagtcaaacc gcgggcaata aggtcttcgt tcagggcata gaccttaatg
     4081 ggggcattac gcagactttc aaggtagcgg ttaccgtcaa ctgcggcagt tacgccatct
     4141 tgcaataata gcagttcgtc tccttcactg agcagacgca gcagcgcagc aaaatccgtc
     4201 agccagggtg aacgatgtaa cgtgtgcagc ataaacgcct caaaacctca aaataacatc
     4261 gtagttggcg agttcgcggc gtaaggcatc tgcttcgagc ggcgtggctt cgacaacaaa
     4321 gggtgtctgc ggatctaacc cgcgttcgcg cagtgaagcc gcacaaaccc agcactgttc
     4381 aatgtcgtac agacccaaca atttaaaagt ggcaatgtaa tcacgcgcca gcactgcatc
     4441 gggcttttgt cctgacagca gctgaaaaac gccatcagca ataaagaaga cggccagatc
     4501 gtcagttaat gcggaagttg ccagtaaagc atctaaacct tcccgaccag cagctgtacc
     4561 atgaggtgca gtagaaaaaa caaacgcaat tcgtttcatc agaactgtac caccctgtca
     4621 caggtcagcg aggcttccgc cagcgcgcca agtccgctta aggtaaatcc cggctgaagg
     4681 tttgacgaag ccagccccag ccttcctgct tcagtttcat cgacgatacc gcggcgtaac
     4741 gctgccgcta cgcagatatt cagcgccaca ccatgttgcg cattcaactg ttgccagccg
     4801 cgcacaaggt caaattcgtc acttgccggt gaggtcaatt ggttagcgtt atagacccct
     4861 tcccgataga aaaagacgct gcttaactca tggccttcta ctatcagcgc ctgcgcaaac
     4921 tgaaaagcac tactcgcctg ttgcgtaccg tatgctggcc cggtcaccac gatggcaaaa
     4981 cgcattactt atcttgcccc tggaaatcac cgcttttgaa ctggcggatg tagagataga
     5041 cagtgtgttt ggagatgttc aggcggtcag caacctggtt gatcgcgtct ttaatatcga
     5101 agatcccttt ctcgtagaga ttcagcacga tctgacgatt tttggcgtta ttagaaacat
     5161 tgcgatcggc attcacttct tcgatggtga actccagcgt ttgggtaacc agatcttcaa
     5221 cagaagaggc aaagttgacg cttgaaccga catccggggt ttctggcggc acaaaagtgc
     5281 tcataatctg cgagaaggga acatcaagat tcatattgat gcacagcaga ccaattacac
     5341 gctgttcgcg gttacgaatc gcgatagtca gggacttcat taatacgccg cttttggcgc
     5401 gagtaaagta gcatttagaa acgctgctat ccgctcccgt catatcgtgc agcatacgta
     5461 gcgccaggtc agtaattggc gaaccaatct tccggcctgt atgttcaccg ttagcaatgc
     5521 gaatggctga acattttaga tcctgcaaag agtgcaaaac gatttcacag tgggagccaa
     5581 taagcatcgc taacccgtcc accaccgctt cgtaggattt cagaatatca aaatcggtct
     5641 ggtcgaaagg acgttgatcc agtaaatcca actcactggt ttcgttggtt aaaagcgacc
     5701 tggacatgaa aaaaaacact ccttttcagg agcctgtcgt taacttttca gggcaggctc
     5761 attaatgatg cgggtaacta aattaataca gcggaggttc cgctttccag cactaattat
     5821 atccggcctg taataaaaaa accgccgcct ggtcaggcgg cggttcttaa tgcttatttt
     5881 ttagcagaat ctgcggcttt cgcatcagct tccggctttg catcagcctt cggcgctggt
     5941 ttcacatcca gcagctctac gtcaaacacc agggtagaat tcggtgggat ccccggaaca
     6001 cccgctttgc cgtaagccag ttctggtgga ataaccagtt tgatcttacc gcctttcttg
     6061 atgttcttca gaccttctgt ccaacccggg ataacaccgt ccagacggaa agaaagcggt
     6121 tcaccacggg tgtaagagtt gtcgaactct ttaccgtcga tcagcgtacc tttgtagttc
     6181 actacaacag tatcgctgtc tttcggtgct tcgcctttac cggcttctac tacctgataa
     6241 accagaccag ttgaagaggt tttcacacct ttctctttgg caaatttctc gcggtactct
     6301 ttaccttttg cttcgttatc agccgcgtct ttttccatct tcgcctgagc agaagacttc
     6361 acgcgagctt cgaatgcttg cagagtctgt tcgatctctt ggtcggagag tttgctctta
     6421 tcagcaaatg catcctgaac accagcgatc agctgatctt tatccagttt gatgcccagt
     6481 ttttcttgtt ctttaagaga gttttccatg taacgaccca gcgaagcacc cagtgcataa
     6541 gctgatttct gatcgtcatt tttgaacgct gctttgctgt cagcagttgt agcaggtttt
     6601 gcagcttcag cagcaaaagt gattggtgca tgcagggcaa cggccattgt ggtcgccagc
     6661 agcgttactt taaacagtga tttcatccat atctccagga tcggggcatc tcaccccagg
     6721 gttaactatt atcagaaggg tactataaag cgttgtcgaa caaatctaca tacagacacg
     6781 ccctattatc atctattttc agactctttt tgtttaaatt agtttcgatg accgcgaaat
     6841 gagtgctgtc tcgggcagca aagttaagta gaatccgcgg cgaccattcg acaaaagagg
     6901 tgaatcatgc aggatttatc attggaagca cgcctggcag agctggagag ccgactggct
     6961 tttcaggaaa tcaccattga agaactgaac gtcacggtga ccgctcatga aatggagatg
     7021 gcgaaactgc gcgatcatct gcgtctgctg accgagaagt taaaagccag ccagccgtcg
     7081 aacatcgcgt cgcaggctga agaaacgcca ccgccacatt attgaggcgt aaaaaaagcg
     7141 gggattcccc gcttttttgt caccttttcg gtattagtgg caaccacaac cgccgttgcc
     7201 tttaccgcca cagcagcctt cgccaccgtg ttcatgaccg tgatcgtggc catgaccgcc
     7261 gcagcaaccg tcgtggtcgt gatcgtggtg atgatcgtgc gcgccgtgaa cgtggccatg
     7321 agccagttct tcttcggtcg cttcgcgaat cgccacaact tcaacgttga atttcaggtt
     7381 ctgaccggcc agcatgtggt taccatcaac cacgacgtga tcgtcttcaa ccgcagtgat
     7441 ttcaaccggt accggcccct ggtcggtttc agccaggaaa cgcataccta cctgcagttc
     7501 atcaacgccc ataaatacgt ctttaggaac acgttgcacc aggttttcgt cgtactgacc
     7561 gtaagcgtcg ttcgcgccaa cagcgacatc aaatttgtcg ccaacttcat gaccttccag
     7621 cgccgtttcc aggccagaga tcagggaacc gtgaccatgc aggtagtcca gcggcgcact
     7681 caccggagac tcatcaacca acacaccgtc ttctgtacgt acctgatagg ccaggctgac
     7741 caccaggtct tttgctactt tcatgatatc tcctgagcat gggaagaata gtggcgcaga
     7801 ttgtagcgga attctgcagc cgtgtactca ctagcttaaa aaaactcggc gcatatcgct
     7861 agtccggatg aaaaatcccg atcacttgct catctttgcg aacgtgatcg cgggcttctt
     7921 tgtctgcttc tcgcatctga tgtccgcact taacacattc aacaatatca atattatttt
     7981 cgcgccacat cgccattgaa tcctgcgcct gacaggccgg gcattttgcg cccgcgataa
     8041 aacgttttcg gattgccatc tttaccctct actcaaattc atcccagccg tccagctggc
     8101 gtcgttcttg ttgcatttca cgctggaaaa tctcttccag ttcgcgtcgg gcttccctgg
     8161 cgcgagaaat ttgtacggta tcagcatgca tcgggattaa ctcccgcagc attcgcatat
     8221 ccaggcggcg aaaatgcagt tgcgcccgct gtgcctgatg cggatgcatg ccaagcgtga
     8281 ccagcgtctt gcgccccagc tctaacgcac tggagaatgt ttcacgggaa aactgcgtca
     8341 cccctgcctg taataactca tgcgcttcca cacgtccgcg cgctcgcgca agaatatgca
     8401 aatgcggaaa gtgctgctgg catatttcca ccagcttcat ggtgtcttcc ggctcgttac
     8461 aggtaatgac gatagactca gcggcctctg cacccgcaga acgtaaaaga tcgacctgcg
     8521 tggcgtcgcc gtaataaact ttgtagccat atttacgcat caggttaacg gcgctgatat
     8581 cccgctccag cacggtaatg cgcattttat ttgccatcag caaacgacca atcacctgac
     8641 caaaacgccc gaagcccacg acaatgacct ggggtttatc atcgttgacc cacggttttt
     8701 cgtcttcttc ttccggtccg ttaaactggc gggatagcca tttatccacc agcttcatca
     8761 gcaacggcgt ggtcatcatg gaaagcgtca ccgtcaccag caacagcgcc atctggtcgc
     8821 cctggaataa acgttgtgaa gaagcggtag aaaagaggac aaaggcaaac tcaccgccct
     8881 gactcaacac gccagcaaac tgcatccgct ctgagctacg cacgccatac aatcgcgcca
     8941 gcagatacag cacgagaatt ttcaccgcca ccagcacaac cacgcttatc actacccaca
     9001 acagatgggt ataaagcacc ccgaggttga gcgacatgcc gacagagata aagaacaaac
     9061 cgagcagcaa tcctttgaag ggatcgatag ccgtttccag ttcatggcga tattcacttt
     9121 ccgccagcag cacgcccgca ataaatgtac cgagcgccat cgacagcccc agcgcatcca
     9181 taaacaatgc ggaacccaac accagcagca gcgtcgcggc ggtgaacact tcccgcacgc
     9241 cagaagctgc aataaagcgg aataccggac gcagtaaata gcgcccacca atcagcatgc
     9301 cgacaaacgc cagcaccttc atgccgatct tcatccagtc gaaatgttcg tctgccgaac
     9361 ccgccaacaa cggcactaac gccagtgccg ggatcaccgc cagatcctga aacagcagta
     9421 ccgaaaaccc gagctgcccg gattcgctgc gattcatccc tttctcacgc atcaattgca
     9481 acgccattgc ggtcgaagac atcgcaaggc caatgccacc gaccaccgcc gcctgccagg
     9541 cgaaatccgt cagcatcaat aatcccgcca gcaacgccgc gcttaacagc acctgtgccg
     9601 cgcctacgcc aaaaatcgaa cgccgcagtt gccaaagctt ggagggattc aactcaaggc
     9661 cgatgataaa catcaggaat accacgccga gttccgaaaa gtggaggatc tcatcgacgt
     9721 cgctaataaa ccccagcccc cacgggccaa ttgcaatccc tgccagcaaa tatcccaaca
     9781 cagcgccaat ccccagccgc gatgccaacg gcaccgcagc caccgccgcg aagagaaaca
     9841 gcactcctgc gagtaaaaaa tcggaacctt ccatcagcgg cctcctggag acagcggatt
     9901 tgccagccag tcaccgtagg ctctggcgtg gctcgccagc tcctgtgcgc tttgccgtct
     9961 cgcccagtaa ataatgatgg gacttaacca atgcatccgg cacatgcccg ccgccagttc
    10021 aaagggacgc agaacgtcgc tcatggggta gcgattcaat gcgtcataac ggtaagcact
    10081 ttccggctcg ccggtggtga tcacgctacg ccagtacttt cccgccagtt ggtttcctcc
    10141 cggcccgctg gcaaaaccac ggcttaatac ccggtccagc cactctttca gtagcgccgg
    10201 gcagctatag gtataaagag gatgctgaaa gacaatcacc tcgtgctcgc gcagtaatgc
    10261 ctgctcacgg gggatatcaa taaaaaaatc gggatagtgc gcataaaggt cgtgcacggt
    10321 aacattgctg agctgcgtgg ccggtttaag cagtacccgg tttgccaccg agtcctgaga
    10381 ttccggatgg gcaaacagca gcaaaacttt cgctggctga gacatcatcc ccctcccggt
    10441 atggtttttg tgtatagtcg ctgttttggg ctaccattgc gcccggtgcg gcagctcgcc
    10501 catacattac attatcataa tgataagtta acatagtctg aacatacggc accttatgat
    10561 tgttttctcc tcgttacaaa ttcgtcgcgg cgtgcgcgtc ctgctggata atgccaccgc
    10621 caccatcaac cctgggcaga aagtcggcct ggtgggtaaa aacggctgtg gtaaatctac
    10681 cctgctggca ttgctgaaaa atgaaatcag cgccgacggc ggcagctaca cctttccggg
    10741 aagctggcaa ctggcgtggg tgaatcagga aacgccggcg ttaccgcaag cggcgctgga
    10801 atatgtcatt gacggcgacc gtgaatatcg tcaactagaa gcgcagctac acgacgccaa
    10861 cgaacgtaac gacgggcacg ccattgcgac cattcatggc aagctggatg ctattgacgc
    10921 atggagtatt cgctcccgtg ccgccagcct gctgcacggc ctcggtttca gcaatgaaca
    10981 actggagcgc ccggtaagtg atttctccgg tggctggcgt atgcgtctta accttgccca
    11041 ggcgctgatt tgccgttcag acttgctgct gctcgacgaa ccgactaacc acctcgatct
    11101 cgatgccgtt atctggctgg aaaaatggct gaagagctat cagggcacgc tgatcctgat
    11161 ctctcacgac cgcgacttcc tcgatccgat cgtcgataaa attattcata tcgaacaaca
    11221 aagcatgttc gagtacaccg gcaactacag ttcgtttgaa gtacagcgcg ccacccgtct
    11281 ggcgcagcaa caagcgatgt acgaaagcca gcaggaacgc gtagcgcatc tgcaaagtta
    11341 tatcgaccgt ttccgtgcca aagccaccaa agcgaagcag gcccagagcc gcattaagat
    11401 gctcgagcgt atggagctaa ttgcccccgc gcacgtcgac aacccgttcc gctttagctt
    11461 ccgcgcgccg gaaagcctgc caaatccgtt actgaagatg gaaaaagtca gcgcgggcta
    11521 tggcgatcgc attattctcg actcgattaa actgaacctg gtgcccggct cgcgcattgg
    11581 tctgttaggc cgcaatggcg cgggtaaatc gacattaatc aaactgttag ccggtgaact
    11641 tgcgccagtc agcggtgaaa ttggtctggc gaaagggatc aaactcggct acttcgccca
    11701 gcatcaactt gaatacctgc gcgccgacga atcacctatt caacatctgg cacgtttagc
    11761 gccgcaggag ctggaacaaa aactgcgtga ctacctcggc ggctttggtt tccagggcga
    11821 taaagtaacc gaagaaacgc gccgcttctc cggtggggaa aaagcccgcc tggtgctggc
    11881 attaattgtc tggcagcggc cgaatctgct gctgctcgac gaaccgacta accaccttga
    11941 cctcgacatg cgtcaggcac tcaccgaagc attaatcgag tttgaaggcg cgctggttgt
    12001 cgtttcgcac gaccgtcatt tgctgcgttc caccactgac gatctctacc tggttcacga
    12061 tcgtaaagtc gaaccgttcg acggcgatct ggaagattat caacagtggt tgagcgacgt
    12121 acaaaagcag gaaaaccaga ccgacgaagc gccaaaagag aatgcgaaca gcgcccaggc
    12181 acgtaaagat cagaagcgtc gggaagctga gctgcgtgcg caaacccagc cactgcgtaa
    12241 agagattgcc cgtctggaaa aagagatgga gaagctgaac gcgcaactgg cgcaggcgga
    12301 agagaaactc ggcgacagcg aactgtatga ccagagccgt aaagcggagt tgaccgcctg
    12361 cctgcaacag caagccagcg ccaaatccgg cctggaagag tgcgaaatgg catggctgga
    12421 agcccaggag cagcttgagc agatgctgct ggaaggccaa agcaactgat ggcgcagata
    12481 acgacgaccg atgccaatga attcagcagc agtgctgaat tcatccccat gcgcggcttt
    12541 agcaattgtc atctgcaaac catgctgccg cgtctgtttc gtcgtcaggt gaaattcacc
    12601 ccgtactggc agcggctgga gttgcccgac ggcgattttg tcgatcttgc gtggagtgaa
    12661 aaccctgcac aggcgcaaca taaacctcgt ctggtggtgt ttcacgggct ggaaggcagt
    12721 ctcaacagcc cttacgccca cggtctggtt gaggcggcgc aaaagcgcgg ctggctgggc
    12781 gtggtgatgc attttcgcgg atgcagcggt gaaccaaacc gtatgcaccg catttaccat
    12841 tcgggcgaaa ccgaagacgc cagttggttt ttacgctggc tgcaacgcga atttggtcat
    12901 gcgccaacgg ctgccgtcgg ctattcgctc ggcggtaata tgctggcctg tttgctggca
    12961 aaggaaggca atgatctccc ggttgatgcg gcggtgattg tctctgcgcc gtttatgctg
    13021 gaagcctgta gctatcatat ggaaaagggc ttttcccgcg tttatcagcg ttacttgctg
    13081 aacctgttaa aagccaatgc cgcgcgcaag ctggcagcct accccggaac gctgccgatt
    13141 aatctcgcgc agttaaaatc ggtacgtcgc atccgtgaat ttgacgatct catcaccgcc
    13201 agaattcacg gctacgccga cgctatcgac tattatcgtc agtgtagcgc catgccgatg
    13261 ctgaaccgga tcgccaaacc gacgctgatt attcacgcca aagacgatcc gtttatggat
    13321 catcaggtga tcccgaaacc ggaaagtctc cccccgcagg tggagtatca actgactgaa
    13381 catggcggtc atgttggctt tattggcggt acattacttc atccgcaaat gtggctggag
    13441 tcacgcattc ctgactggtt aacaacgtat ctggaggcga aatcatgttg attccgtggc
    13501 aagacctctc ccccgaaacg ctggaaaatt taattgaaag ctttgtgtta cgtgaaggca
    13561 ccgattatgg tgaacatgag cgtacacttg aacagaaagt cgccgacgtc aaacgccagc
    13621 tacagtgcgg agaagcggtg ctggtatggt cggagctgca cgaaacggtc aatattatgc
    13681 cgcgcagtca gtttcgcgaa taacctgcaa ccgtcggcgg acgagataat aatgatagct
    13741 aagtcatgga gttactatgt ctgccaaaca tccggtcatt gcggtaacag gatccagcgg
    13801 cgcggggacc accaccacca gcctcgcgtt tcgtaaaata ttcgcgcagt taaatctgca
    13861 tgcagctgag gtggaaggcg acagttttca ccgttacacc cgcccggaaa tggacatggc
    13921 gatccgcaaa gcgcgcgacg ccgggcggca tatcagctac ttcggccccg aggctaacga
    13981 cttcggcctg ctggaacaaa ccttcattga atacggtcag agcggcaaag ggaaatctcg
    14041 caaatatctg catacctacg acgaagccgt accgtggaat caggtaccgg ggacattcac
    14101 cccctggcaa cctttaccgg aacccactga tgtactgttt tatgaaggtt tacacggcgg
    14161 cgtagtcacg ccacagcata acgttgcgca gcatgtggac ttactggtcg gcgtggtgcc
    14221 tatcgttaac cttgagtgga ttcaaaaact gatccgcgac accagcgagc gcgggcactc
    14281 acgagaagca gtgatggact cagtagtgcg ttcaatggaa gactatatca actacatcac
    14341 accgcagttt tcccgcaccc atcttaactt ccagcgcgtt cccaccgtcg acacttcaaa
    14401 cccgttcgcg gcaaaaggta tcccgtcgct cgatgaaagc tttgtggtga tccattttcg
    14461 taatctggaa gggatcgatt tcccctggct gctggcgatg ttgcaaggct cattcatttc
    14521 ccacatcaat acgttagtgg taccgggcgg caaaatgggt ctggcaatgg aattaattat
    14581 gctgccgctg gtgcaacgat tgatggaagg aaagaaaatc gagtaactct gctattacgc
    14641 cggataaaat actatccggc ttcacaacgg gatagttaag tcacgcggca accacttcat
    14701 acgagtgagt aatattcacc gctttttcca gcatcaacgc cactgaacaa tatttctcgg
    14761 cagagagatc aaccgcacgc gcaaccgctg cgtctttcag gtcgcgaccg gtgacgataa
    14821 aatgcagatt aatgtgcgta aacaggcgtg gtgcctcttc gcggcgttca gaggtcaatt
    14881 ttacttcaca atcgaccaca tcctgacgcc ctttttgcag gatcgaaacc acatcgatgg
    14941 cactgcaacc acccgccgcc atcaacacca tttccatcgg acttggtgct ttatcgcctg
    15001 agttgccgtc cattaaaatc tgatgaccag aggcggattc gcccagaaaa gttaaccctt
    15061 cgacccactt cactcgcgct tgcatttttg ctactccact gcgtcaattt tcctgacaga
    15121 gtacgcgtac taaccaaatc gcgcaacgga aggcgacctg ggtcatgctg aagcgagaca
    15181 ccaggagaca caaagcgaaa gctatgctaa aacagtcagg atgctacagt aatacattga
    15241 tgtactgcat gtatgcaaag gacgtcacat taccgtgcag tacagttgat agccccttcc
    15301 caggtagcgg gaagcatatt tcggcaatcc agagacagcg gcgttatctg gctctggaga
    15361 aagcttataa cagaggataa ccgcgcatgg tgcttggcaa accgcaaaca gacccgactc
    15421 tcgaatggtt cttgtctcat tgccacattc ataagtaccc atccaagagc acgcttattc
    15481 accagggtga aaaagcggaa acgctgtact acatcgttaa aggctctgtg gcagtgctga
    15541 tcaaagacga agagggtaaa gaaatgatcc tctcctatct gaatcagggt gattttattg
    15601 gcgaactggg cctgtttgaa gagggccagg aacgtagcgc atgggtacgt gcgaaaaccg
    15661 cctgtgaagt ggctgaaatt tcgtacaaaa aatttcgcca attgattcag gtaaacccgg
    15721 acattctgat gcgtttgtct gcacagatgg cgcgtcgtct gcaagtcact tcagagaaag
    15781 tgggcaacct ggcgttcctc gacgtgacgg gccgcattgc acagactctg ctgaatctgg
    15841 caaaacaacc agacgctatg actcacccgg acggtatgca aatcaaaatt acccgtcagg
    15901 aaattggtca gattgtcggc tgttctcgtg aaaccgtggg acgcattctg aagatgctgg
    15961 aagatcagaa cctgatctcc gcacacggta aaaccatcgt cgtttacggc actcgttaat
    16021 cccgtcggag tggcgcgtta cctggtagcg cgccattttg tttcccccga tgtggcgcag
    16081 actgatttat caccccgata tcaactatgc acttcgacaa acgctggtgc tatgtttgcc
    16141 cgtggccgtt gggttaatgc ttggcgaatt acgattcggt ctgctcttct ctctcgttcc
    16201 tgcctgttgc aatattgcgg gccttgatac gcctcataaa cgttttttca aacgcttaat
    16261 cattggtgcg tcgctgtttg ccacctgtag cttactgaca cagctgctac tggcaaaaga
    16321 tgttcccctg ccctttttgc tgaccggatt aacgctggta cttggcgtca ctgctgagtt
    16381 ggggccattg cacgcaaaat tgcttcccgc atcgctgctg gcagccattt ttaccctcag
    16441 tctggcggga tacatgccgg tctgggaacc gttgctcatc tatgcgttgg gcactctctg
    16501 gtacggattg tttaactggt tttggttctg gatctggcgc gaacaaccgc tgcgcgagtc
    16561 actaagtctg ctgtaccgtg aactggcaga ttattgtgaa gccaaataca gcctgcttac
    16621 ccagcacacc gaccctgaaa aagcgctgcc gccgctgctg gtgcgccagc aaaaagcggt
    16681 cgatctaatt acccagtgct atcagcaaat gcatatgctt tccgcgcaaa ataatactga
    16741 ctacaagcgg atgctgcgta ttttccagga ggcgctggat ttacaggaac atatttcggt
    16801 cagtttgcat cagccggaag aggtgcaaaa gctggtcgag cgtagccatg cggaagaagt
    16861 tatccgctgg aatgcgcaaa ccgtcgccgc tcgcctgcgc gtgctggctg atgacattct
    16921 ttaccatcgc ctgccaacgc gttttacgat ggaaaagcaa attggcgcac tggaaaaaat
    16981 cgcccgccag catccggata atccggttgg gcaattctgc tactggcatt tcagccgcat
    17041 cgcccgcgtg ctgcgcaccc aaaaaccgct ctatgcacgt gacttactgg ccgataaaca
    17101 gcggcgaatg ccattacttc cggcgctgaa aagttatctg tcactaaagt ctccggcgct
    17161 acgcaatgcc ggacgactca gtgtgatgtt aagcgttgcc agcctgatgg gcaccgcgct
    17221 gcatctgccg aagtcgtact ggatcctgat gacggtattg ctggtgacac aaaatggcta
    17281 tggcgcaacc cgtctgagga ttgtgaatcg ctccgtggga accgtggtcg ggttaatcat
    17341 tgcgggcgtg gcgctgcact ttaaaattcc cgaaggttac accctgacgt tgatgctgat
    17401 taccaccctc gccagctacc tgatattgcg caaaaactac ggctgggcga cggtcggttt
    17461 tactattacc gcagtgtata ccctgcaact attgtggttg aacggcgagc aatacatcct
    17521 tccgcgtctt atcgatacca ttattggttg tttaattgct ttcggcggta ctgtctggct
    17581 gtggccgcag tggcagagcg ggttattgcg taaaaacgcc catgacgctt tagaagccta
    17641 tcaggaagcg attcgcttga ttcttagcga ggatccgcaa cctacgccac tggcctggca
    17701 gcgaatgcgg gtaaatcagg cacataacac tctgtataac tcattgaatc aggcgatgca
    17761 ggaaccggcg tttaacagcc attatctggc agatatgaaa ctgtgggtaa cgcacagcca
    17821 gtttattgtt gagcatatta atgccatgac cacgctggcg cgggaacacc gggcattgcc
    17881 acctgaactg gcacaagagt atttacagtc ttgtgaaatc gccattcagc gttgtcagca
    17941 gcgactggag tatgacgaac cgggtagttc tggcgatgcc aatatcatgg atgcgccgga
    18001 gatgcagccg cacgaaggcg cggcaggtac gctggagcag catttacagc gggttattgg
    18061 tcatctgaac accatgcaca ccatttcgtc gatggcatgg cgtcagcgac cgcatcacgg
    18121 gatttggctg agtcgcaagt tgcgggattc gaaggcgtaa tgcaggctaa accatcggat
    18181 aaggcattca cgccgcatcc gacatctttt gcctgatgct tcgtatttac gccccaacca
    18241 ccttcgccac cgcgtgggcg aaacgttgca tcccttcatc gatatccgca tcttccacca
    18301 ccagcgacgg tgcaaaacgc atcacatccg gtccggcatt cagcaccatt acgccagcct
    18361 ctgcgcccgc atacaggaaa tcacgcgccc gacctttgta ctgtggtttc agctctgcgc
    18421 caatcaacag ccccataccg cgaatatcgc taaacacatc gtactgctga tcgatcttct
    18481 gcagatggtc aacaaaacgc tggcgtttcg cctgaatgcc ttccagcact tcaggggtat
    18541 tgatgatatc aaacgccgcc cccgctactg cacaggccag aggattaccg ccgtaggtgg
    18601 aaccgtgaga accaggatga aacgcagaag caatttctgc cgtggtcagc atggcgctaa
    18661 tcgggaagcc gccgcctaac gctttcgcag aggtcagaat atccggcgtc acgccgtagt
    18721 gcatgtaagc aaacaaatcg ccggtccgcc ccatcccgca ctgtacttca tcaaacacca
    18781 ataatgcctg atgttgatcg cacagctcgc gcaagccctg caaaaactct ggcgtcgctg
    18841 ccgtcacacc gccctcgccc tggatcggct caaccaccac cgcacaggtg tgatcatcca
    18901 tcaccgcttt cactgcgtgg agatcgttaa aaggaacgtg gatgatgtct gccggttttg
    18961 gcccaaagcc gtcggaatat tttggctgcc cgcccaccga gacggtaaac agcgagcgac
    19021 catgaaaagc gttatggaag gcaataattt tggttttgaa cggactatga cgcacacagg
    19081 cgtaatggcg tgccagttta aaggcggttt cgttagcttc cgtgccggag ttcataaaca
    19141 ccacgcgttc ggcaaacgtc gcctcaatca gtttgcgccc aagacgcagc gccggttcat
    19201 tggtgaaaac gttactgata tgccacagag tttcgccctg ggtttttaac gcgttcacca
    19261 gcgcaggatg gcaatggccc aacgccgtaa ctgcaatgcc acccgcgaaa tcgacatact
    19321 ccttgccttg ctgatcccag attcggctgc cctgaccttt taccggaatg aactctgccg
    19381 gagcataaat cggcaggatc acttcatcga aagtcgcgcg tgtaattgct gtttgttcaa
    19441 ttgccatctc atgatcaccc tgttacgcat aaataaatgt gaaattataa tcacaaaata
    19501 tgcataaaaa atcattgaat ggcaatcaga aatcagcgat gcaggaaatt agccagcagt
    19561 tgatgtcctt gttcgctaag aatactttct ggatggaact gcacaccttc cagatcccac
    19621 tggcgatagc gaatccccat aatctcgcgg gtttcgctcc aggccgtcac gtcaaagcat
    19681 gctggtaatg agccaggttc caccaccagc gaatggtagc gtgtcacggt aagtggattt
    19741 gccagccccc gaaatacgcc ctcaccgtta tgtgtaatcg gcgaggtttt gccgtgcatg
    19801 acctttgcgg cgcgcacaac tttgccgcca aatgcctgcg ccattgcctg atgaccgagg
    19861 cagacgccaa gaatcggcaa gcgcccggca tagtggcgaa taacgtcaag ggagatcccg
    19921 gcttcatctg gcgtacaggg gccgggtgat atgacaattt tttgtggttt aagggcgtcg
    19981 atatccgcca gcgtcaacgc atcgttgcgc ttaaccagca catccgcccc cagttcacaa
    20041 aagtactggt agaggttcca ggtaaaagaa tcgtagttat ctataagcag gatcatggcg
    20101 gctccggtac gaaagaaccg cgctatttta ctcagattcc ccggcttcgc ttaccacttt
    20161 gctaaatatc atctgcagcg cggtgagatc ccccattgcc ccactctgat ttgcctgatt
    20221 ccaggcctct ttttcgatac cctgccagct cagttgatac ccggcatgaa tcgccagttg
    20281 ctcgaagaag atccgctgtg ccagaccact tcccacccgg aagggatgca gcacgttgat
    20341 ttcacaatag taatgcgcca gccgctcgac gaacttcgct ttctccaggc caaccagata
    20401 accttcttcc tccagatcct gcatcagggc attgccctct ttttcgatat aagcaaagtg
    20461 gcagaacggc gtatcgccct gataaatatc aacttcacgc agttgccccg cccagtcgaa
    20521 aatatcctga tacagctggc gatggatagt gcgcaaatgc ggtaaaccgc gcaccagcgg
    20581 accaagctca atggtcgcag cacgcagcgc cgtcatttcg taagcggcct gttccagccg
    20641 ctgctgctgg tggatgttca gccggttacg catgatatca aggccaggat aaagatacgg
    20701 atcgcgccct tcgccgaatt tatcgctcat agtgcctcct cagctcttca aggcgcgcta
    20761 aagcctctgc ggcagtaaga gtgactaaag gcatctcgac gccttcaaga cggcgactgg
    20821 cctggaaatt acgattacgc tgaagctccc agagacggga cttttgctta tcggtgagtt
    20881 tcttcacttg atgcctccct gaacgtgtcc atttgccaca agtataagcg gcaaatccgg
    20941 gttacgccgg gagagcagaa gcgcgggcaa gattgcccgc gcgaaaaatc attacggcag
    21001 gactttagcg gaaaggataa ctaccggttt tgacggcaca ttctggtacg gaccaacgtc
    21061 atgagtcggc acctgggaaa tcttatcggc aacgtccatg cctttcacca ctttaccaaa
    21121 taccgcgtaa ccgaaatcac gctgaccatg gtcaaggaag gcgttatcgg caacgttgat
    21181 aaagaactgg ctggtggcgc tgtctttgtc agcggtacgc gccatcgcga tggtgccacg
    21241 cgtgttgcgc aggccgttat cggcttcatt tttgattggc gggtttggtt ttttctgctg
    21301 catctgctcg gtgaaaccgc cgccctgaat cataaagcca ggaatgacgc ggtgaaaggt
    21361 agtgttgtta taaaaaccgc tgttcacata atcgacaaag ttttgcacag acactggcgc
    21421 tttttgttta tccagctcca gttcgatgtt accagctgag gttgtcaaca atacgtgcgg
    21481 gtcccctttc gctgccattg ctgcgggaga aagagcagaa agagcgaaaa cagcagccat
    21541 cgccgccagg gtcgatttga acatgagatt tccttaacaa acagagcaga aaaaaagcaa
    21601 gtgaagagat tctaaatagc cttcaagaac aaggccatcc ctttacctaa ttttacgtat
    21661 ctgaaatata tgtaacctga cgatcacttt ttattccgtg atcaaaatca cctcttaaaa
    21721 tgcaatttag caaccgattg caataaaaca tttaaacaga tcacaaaatc acctaaaatc
    21781 gcccgctcgc ggcatatccc gctatgccat tttatttttg aacgcaggcc aatcatgact
    21841 aacagcaatc gcatcaagct cacatggatt agctttctct cctacgcact gactggtgcg
    21901 ttggttattg tcaccgggat ggtgatggga aatatcgccg attatttcaa tctgcctgtt
    21961 tccagtatga gtaacacctt caccttcctc aacgccggca ttttaatctc tatcttcctc
    22021 aacgcctggc tgatggaaat cgtcccgttg aaaacgcagt tacgttttgg ctttctcctg
    22081 atggtgctgg cggttgccgg tttgatgttc agccacagcc tggcgctgtt ctcggcggcg
    22141 atgttcattc tcggggtggt cagcggcatc accatgtcga ttggtacatt cctggtaaca
    22201 caaatgtatg aagggcgtca gcgcggttcc cgcctgttat ttaccgactc cttcttcagt
    22261 atggctggga tgattttccc aatgatcgcc gcgtttctac tggcgcgcag cattgagtgg
    22321 tactgggttt atgcctgcat cgggctggtg tatgtcgcta tttttattct gaccttcggc
    22381 tgtgagttcc cggcgctggg caaacatgcg ccaaaaacgg atgctccggt agagaaagaa
    22441 aagtggggga tcggcgtact gtttctctct gttgcggcgc tgtgctacat cctcggtcag
    22501 ttaggtttta tctcctgggt gcctgagtat gccaaaggcc tgggcatgag cctgaacgac
    22561 gcgggcacgc tggtgagtaa cttctggatg tcatacatgg tcggcatgtg ggcgttcagc
    22621 tttattcttc gcttctttga tttgcaacgc attctgaccg tactggctgg tctggctgcg
    22681 attctgatgt acgtctttaa caccggaaca ccagcacata tggcgtggtc aattctcgct
    22741 ctgggcttct tctccagcgc gatctatacc accatcatca ccctgggttc acagcagacc
    22801 aaagtaccgt cgccaaaact ggttaacttt gtcctgacct gcggaaccat cggtactatg
    22861 ttgacctttg tggttaccgg cccgattgtt gaacatagcg gtccgcaggc ggcactgctt
    22921 acagcaaacg gtctgtacgc tgtcgtcttt gtgatgtgct tcctgttagg tttcgtcagc
    22981 cgtcaccgtc agcataacac cctgacctct cattaattgc tcatgccgga cggcactctc
    23041 gtcgtccggc cttttcctct cttcccccgc tacgtgcatc tatttctata aacccgttca
    23101 ttttgtccat tttttgcaca aacatgaaat gtcagacaat tccgtgactt aagaaaattt
    23161 atacaaatca gcaatatacc ccttaaggag tatataaagg tgaatttgat ttacatcaat
    23221 aagcggggtt gctgaatcgt taaggtaggc ggtaatagaa aagaaatcga ggcaaaaatg
    23281 agcaaagtca gactcgcaat tatcggtaac ggtatggtcg gccatcgctt tatcgaagat
    23341 cttcttgata aatctgatgc ggccaacttt gatattaccg ttttctgtga agaaccgcgc
    23401 atcgcttatg accgcgtaca cctctcgtct tacttctctc accacaccgc cgaagagctg
    23461 tcgctggtgc gcgaaggctt ctacgagaaa cacggcatca aagttctggt cggcgaacgc
    23521 gctatcacca tcaaccgtca ggagaaggtg attcactcca gcgccgggcg caccgttttt
    23581 tatgacaagc tgatcatggc gaccggttcc tacccgtgga tcccgccaat caaaggttct
    23641 gatactcagg actgctttgt ctatcgcact attgaagacc tcaacgccat tgaatcctgc
    23701 gcccgtcgca gcaaacgcgg tgccgttgtt ggtggcggcc tgttaggtct ggaagccgct
    23761 ggcgcgctga aaaacttagg tattgaaacc cacgttatcg aatttgcccc tatgctgatg
    23821 gcggaacagc ttgatcagat gggtggcgag cagctgcgtc gcaaaatcga aagtatgggc
    23881 gtgcgcgttc acaccagcaa aaacaccctt gagattgtgc aggaaggtgt cgaagcgcgt
    23941 aaaaccatgc gttttgccga cggcagcgaa ctggaagtcg actttatcgt cttctctacc
    24001 ggtatccgtc cgcgcgataa gctggcaacc cagtgtggtc tggacgttgc tccgcgtggg
    24061 ggtatcgtca ttaatgattc ctgccagact tccgatccgg atatctacgc catcggtgaa
    24121 tgcgcaagct ggaacaaccg tgtatttggt ctggtagcac ctggctacaa aatggcgcag
    24181 gtcgccgttg accatattct cggtagcgaa aacgcctttg aaggtgctga ccttagcgcc
    24241 aagctgaaac tgctgggcgt agacgtaggc ggtattggtg atgcgcacgg tcgcacgcct
    24301 ggcgcacgta gctacgttta cctcgacgaa agtaaagaga tctacaaacg cctgattgtc
    24361 agcgaagaca acaaaaccct gctcggtgcg gtactggtgg gcgataccag cgactacggc
    24421 aacctgctgc aactggtgct gaatgctatc gagctgccgg aaaacccgga ttctctgatt
    24481 ctgccggcgc actcgggtag cggtaagccg tctatcggtg ttgataaact gccggacagc
    24541 gcgcaaatct gctcctgctt cgacgtcacc aaaggtgatc tgattgctgc catcaacaaa
    24601 ggctgccaca ccgttgcggc gctgaaagct gaaaccaaag cgggtactgg ctgcggtggc
    24661 tgtatcccgc tggttactca ggtactgaac gcggaactgg cgaaacaggg catcgaagtt
    24721 aacaacaacc tgtgcgaaca ctttgcttat tcgcgtcagg aactgttcca tttgatccgc
    24781 gttgaaggca ttaaaacctt cgaagaactg ctggcgaaac acggcaaagg ctacggttgt
    24841 gaagtttgta aaccgaccgt cggttcgctg ctggcctcct gctggaacga atacattctg
    24901 aagccggaac atactccgct gcaggattct aacgacaact tcctcgctaa catccagaaa
    24961 gacggcacct actcggtgat cccgcgttct ccgggcggtg aaatcacccc ggaagggctg
    25021 atggcagtgg gtcgtatcgc gcgtgaattt aatctctaca ccaaaatcac cggctcccag
    25081 cgcctggcga tgtttggcgc acagaaagac gatctgccgg aaatctggcg tcagctgatt
    25141 gaagctggct tcgaaaccgg tcatgcctat gcgaaagcac tgcgtatggc gaaaacctgc
    25201 gtgggtagca cctggtgccg ctacggtgtt ggcgatagcg tcggcctcgg cgtggaactg
    25261 gaaaaccgct acaaaggcat ccgtacgccg cacaaaatga agttcggtgt ctccggctgt
    25321 acccgtgaat gttcagaagc tcagggtaaa gacgtgggta ttatcgccac tgaaaaaggc
    25381 tggaacctgt atgtttgcgg taacggcggc atgaaaccgc gtcatgcgga tctgctggca
    25441 gcggatatcg atcgcgaaac gctgatcaaa tatctcgacc gcttcatgat gttctacatc
    25501 cgtaccgccg acaaactgac gcgtaccgcg ccgtggttag aaaacctcga aggcggcatc
    25561 gattacctga aagcggtgat cattgacgac aaactggggc tgaacgctca tctggaagaa
    25621 gagatggcgc gcctgcgtga agcggtagtg tgtgagtgga ctgaaacggt caatacaccg
    25681 tccgcgcaga ctcgcttcaa acacttcatc aacagcgaca agcgtgaccc gaacgtgcag
    25741 atggtgccag agcgcgaaca gcaccgtccg gcaacgccgt atgaacgtat cccggtaact
    25801 ctggtggagg acaacgcatg agccagtgga aagacatctg caaaatcgat gacatcctgc
    25861 ctgaaaccgg cgtctgcgcg ctgttaggcg acgagcaggt cgcgattttc cgcccgtatc
    25921 acagcgacca ggtgtttgcg atcagcaaca tcgatccgtt cttcgagtcc agcgtgctgt
    25981 cacgcgggct gattgcggaa caccagggcg agctgtgggt cgccagcccg ctgaaaaaac
    26041 agcgttttcg cttaagtgat ggcttgtgca tggaagacga acagttttcc gtcaaacatt
    26101 acgaagcacg agtgaaagac ggcgtggtgc agctgcgcgg ttaatgtttt aacgggaggc
    26161 gcaatgcctc ccctttttgc atggtcctgt aatgaccttc ggtataatgc aggacatttt
    26221 ttaaactttt tgttttattt tttgttttta ttttttaaag gataatcaaa tgtttacaga
    26281 cactattaat aagtgtgcgg ctaatgctgc acgcattgca cgcctgtcgg caaataaccc
    26341 gctcggcttt tgggtcagct ccgccatggc gggcgcatat gtgggtcttg ggatcatcct
    26401 gattttcacg ctcagtaatt tgctcgaccc gtccgtacgt ccgctggtga tgggcgcgac
    26461 ctttggtatc gccttaacgc tggtgattat cgccggttct gaactgttca ccggacacac
    26521 catgttcctc acctttgggg taaaagcggg cagcatcagc cacgggcaaa tgtgggcaat
    26581 cctgccgcaa acctggctgg gtaacctggt cggttccgtc ttcgttgcca tgctctatag
    26641 ctggggcggc ggtagcctgc tgccggtaga taccagcatc gttcactccg tcgcgctggc
    26701 taaaaccact gcaccggcaa tggtactgtt cttcaaaggt gcattgtgta actggctggt
    26761 ttgcctggca atctggatgg cgctgcgcac tgaaggggcg gcgaaattta tcgctatctg
    26821 gtggtgtctg ctggcattta tcgcgtccgg ctacgagcac tctatcgcta acatgacgct
    26881 gttcgcgctc tcctggttcg gcaaccacag cgaagcctac acgctggcgg gtattggtca
    26941 taacctgctg tgggtgacgc tgggtaatac tttatcaggt gccgtattca tgggattggg
    27001 ttattggtat gctacgccga aagcgaatcg tccggttgcg gacaaattta atcaaactga
    27061 aacggctgcc ggttaattac taaggggttt ttacgtggat catttgccta tattttgcca
    27121 attacgcgat cgcgactgtc tgattgtcgg cggtggtgat gtcgcggaac gcaaagcaag
    27181 gttgctgtta gacgcaggcg ctcgcttaac ggtgaatgca ttagcgttta ttccacagtt
    27241 caccgcatgg gcagatgcag gcatgttaac cctcgtcgaa gggccatttg atgaaagcct
    27301 tctcgacacc tgctggctgg cgattgcagc gacggatgat gacacgctta accagcgcgt
    27361 cagcgaagcc gctgaagctc gtcgcatctt ctgtaacgta gtcgatgcgc cgaaagccgc
    27421 cagctttatt atgccgtcga ttattgaccg ctcaccgcta atggtagcgg tctcctctgg
    27481 cggcacctct ccggttctgg cgcgcctgtt gcgcgaaaaa ctcgaatcac tgctgccgtt
    27541 gcatctgggc caggtggcga aatatgccgg gcaattacgc gggcgagtga aacaacagtt
    27601 cgccacgatg agtgagcgtc gccgtttctg ggagaaattg ttcgttaacg accgcctggc
    27661 gcagtcgctg gcaaacaacg atcagaaagc cattactgaa acgaccgaac agttaatcaa
    27721 cgaaccgctc gaccatcgcg gtgaagtggt gctggttggt gcaggtccgg gcgatgccgg
    27781 gctgctgacg ctgaaaggac tgcaacaaat tcagcaggca gatgtggtgg tctacgaccg
    27841 tctggtttct gacgatatta tgaatctggt acgccgcgat gctgatcgcg ttttcgtcgg
    27901 caaacgcgcg ggataccact gtgtaccgca ggaagagatt aaccagatcc tgctgcggga
    27961 agcgcaaaaa ggcaaacgcg tggtgcggct gaaaggtggc gatccgttta tttttggccg
    28021 tggtggcgaa gagctggaaa cactgtgcaa cgcgggtatt ccgttctcgg tggttccggg
    28081 tattaccgca gcttctggtt gctctgccta ttcgggtatt ccgctcacgc atcgcgatta
    28141 tgcccagagc gtgcgcttaa ttaccggaca cttaaaaacc ggtggcgagc tggactggga
    28201 aaacctggcg gcagaaaaac agacgctggt gttctatatg gggctgaatc aggctgcgac
    28261 tattcagcaa aagctgattg aacacggtat gcctggcgaa atgccggtgg caattgtcga
    28321 aaacggaaca gcagtcacgc agcgcgtgat tgacggtacg ctcacgcaac tcggtgaact
    28381 tgctcagcaa atgaacagtc catcgctaat tattattggt cgggttgttg gcctgcgcga
    28441 taaactgaac tggttctcca accattaatt taacccgggc cagagaattc tggccttctt
    28501 aatgactctt tttatatatt cagcaaataa aacatagccc cttaataaat attatctgct
    28561 aatgggttat attcaccgtt acagcgactt acaaaattaa gtcattcgct cactgacgcc
    28621 aattaaatat tcctgtatcg ttcttaacga
//
LOCUS       DAZGJW010000055        28577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZGJW010000055 DAZGJW010000000
VERSION     DAZGJW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.055"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            117..2153
                     /gene="yihQ"
                     /locus_tag="ACHKIK_002369"
     CDS             117..2153
                     /gene="yihQ"
                     /locus_tag="ACHKIK_002369"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO9083531.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDNDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            2199..3584
                     /locus_tag="ACHKIK_002370"
     CDS             2199..3584
                     /locus_tag="ACHKIK_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9083532.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            3627..5030
                     /locus_tag="ACHKIK_002371"
     CDS             3627..5030
                     /locus_tag="ACHKIK_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9083533.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            5098..5790
                     /gene="ompL"
                     /locus_tag="ACHKIK_002372"
     CDS             5098..5790
                     /gene="ompL"
                     /locus_tag="ACHKIK_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO9083534.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(5881..7146)
                     /locus_tag="ACHKIK_002373"
     CDS             complement(5881..7146)
                     /locus_tag="ACHKIK_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9083535.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(7248..8228)
                     /locus_tag="ACHKIK_002374"
     CDS             complement(7248..8228)
                     /locus_tag="ACHKIK_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO9083536.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(8236..8946)
                     /locus_tag="ACHKIK_002375"
     CDS             complement(8236..8946)
                     /locus_tag="ACHKIK_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO9083537.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPKTLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(9163..10986)
                     /gene="typA"
                     /locus_tag="ACHKIK_002376"
     CDS             complement(9163..10986)
                     /gene="typA"
                     /locus_tag="ACHKIK_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO9083538.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            11359..12768
                     /gene="glnA"
                     /locus_tag="ACHKIK_002377"
     CDS             11359..12768
                     /gene="glnA"
                     /locus_tag="ACHKIK_002377"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO9083539.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13087..14136
                     /gene="glnL"
                     /locus_tag="ACHKIK_002378"
     CDS             13087..14136
                     /gene="glnL"
                     /locus_tag="ACHKIK_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO9083540.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            14148..15557
                     /gene="glnG"
                     /locus_tag="ACHKIK_002379"
     CDS             14148..15557
                     /gene="glnG"
                     /locus_tag="ACHKIK_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO9083541.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            15669..15779
                     /locus_tag="ACHKIK_002380"
     CDS             15669..15779
                     /locus_tag="ACHKIK_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO9083542.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(15969..17342)
                     /gene="hemN"
                     /locus_tag="ACHKIK_002381"
     CDS             complement(15969..17342)
                     /gene="hemN"
                     /locus_tag="ACHKIK_002381"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO9083543.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKLW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(17531..18040)
                     /gene="yihI"
                     /locus_tag="ACHKIK_002382"
     CDS             complement(17531..18040)
                     /gene="yihI"
                     /locus_tag="ACHKIK_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO9083544.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(18159..18325)
                     /locus_tag="ACHKIK_002383"
                     /pseudo
     CDS             complement(18159..18325)
                     /locus_tag="ACHKIK_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18622..19254
                     /gene="yihA"
                     /locus_tag="ACHKIK_002384"
     CDS             18622..19254
                     /gene="yihA"
                     /locus_tag="ACHKIK_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO9083545.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(19636..22422)
                     /gene="polA"
                     /locus_tag="ACHKIK_002385"
     CDS             complement(19636..22422)
                     /gene="polA"
                     /locus_tag="ACHKIK_002385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO9083546.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPNDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFSLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            22786..23718
                     /locus_tag="ACHKIK_002386"
     CDS             22786..23718
                     /locus_tag="ACHKIK_002386"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO9083547.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDQLLTSLMSSQRQDK"
     gene            complement(23759..25189)
                     /locus_tag="ACHKIK_002387"
     CDS             complement(23759..25189)
                     /locus_tag="ACHKIK_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO9083548.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(25344..25970)
                     /gene="dsbA"
                     /locus_tag="ACHKIK_002388"
     CDS             complement(25344..25970)
                     /gene="dsbA"
                     /locus_tag="ACHKIK_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO9083549.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(25987..26973)
                     /gene="srkA"
                     /locus_tag="ACHKIK_002389"
     CDS             complement(25987..26973)
                     /gene="srkA"
                     /locus_tag="ACHKIK_002389"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO9083550.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(27050..27319)
                     /locus_tag="ACHKIK_002390"
     CDS             complement(27050..27319)
                     /locus_tag="ACHKIK_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO9083551.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            27389..27973
                     /gene="mobA"
                     /locus_tag="ACHKIK_002391"
     CDS             27389..27973
                     /gene="mobA"
                     /locus_tag="ACHKIK_002391"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO9083552.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            27955..28482
                     /gene="mobB"
                     /locus_tag="ACHKIK_002392"
     CDS             27955..28482
                     /gene="mobB"
                     /locus_tag="ACHKIK_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO9083553.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         7026 a         7137 c         6901 g         7513 t
ORIGIN      
        1 aaaaacagct cagcttctgt aaagttttta tatatcagtt ttatgagaat cattttacat
       61 aagcctgtaa taacaggaag gcaacaatct cgttctcaac tctatggaga ttagttatgg
      121 atacgccacg tccacagtta ttagattttc aatttcatca gaataacgac agttttaccc
      181 tacattttca acaacgtctt attttaaccc atagcaaaga taatccttgt ttatggattg
      241 gctcaggtat agcggatatc gatatgttcc gcggtaattt cagcattaaa gataaactac
      301 aggagaaaat tgcgcttacc gacgccatcg tcagccagtc accggatggt tggttaattc
      361 atttcagccg tggttctgac attagcgcca cgctgaatat ctctgccgac gatcagggcc
      421 gtttattgct ggaactacaa aacgacaacc ttaaccacaa ccgtatctgg ctgcgccttg
      481 ccgctcaacc agaggaccat atctacggct gcggcgaaca gttttcctac ttcgatctgc
      541 gtggcaaacc gttcccgcta tggaccagtg aacaaggcgt tggtcgcaac aaacaaacct
      601 atgtcacctg gcaggccgac tgcaaagaaa atgcgggcgg cgactattac tggactttct
      661 tcccacagcc tacgtttgtc agcacgcaga agtattactg ccatgttgat aacagttgct
      721 atatgaactt cgactttagt gccccggaat accatgaact ggcgctgtgg gaagacaaag
      781 caacgctgcg ttttgaatgt gctgacacat acatttccct gctggaaaaa ttaaccgccc
      841 tgctgggacg ccagccagaa ctgcccgact ggatttatga cggagtaacg ctcggcattc
      901 agggcgggac ggaagtgtgc cagaagaaac tggacaccat gcgtaacgcg ggcgtgaagg
      961 tcaacggcat ctgggcgcag gactggtccg gtattcgtat gacctctttt ggcaaacgcg
     1021 tgatgtggaa ctggaagtgg aacagcgaaa actacccgca actggattca cgcattaagc
     1081 agtggaatca ggagggcgtg cagttcctgg cctatatcaa cccgtatgtt gccagcgata
     1141 atgatctctg cgaagaagcg gcacaacacg gctatctggc aaaagatgcc tctggcggtg
     1201 actatctggt ggagtttggc gagttttacg gcggcgttgt cgatctcact aatccagaag
     1261 cctacgcctg gttcaaggaa gtgatcaaaa agaacatgat tgaactcggc tgcggcggct
     1321 ggatggctga cttcggcgag tatctgccca ccgacactta cctgcataac ggcatcagcg
     1381 ccgaaattat gcataacgcc tggcccgcgc tgtgggcgaa atgtaactac gaagcccttg
     1441 aagaaacggg caagctcggc gagatccttt tctttatgcg cgccggttct accggtagcc
     1501 agaaatactc caccatgatg tgggcgggcg accagaacgt cgactggagt ctcgacgatg
     1561 gcctggcgtc ggttgtcccg gcggcgctgt cgctggcaat gaccggacat ggcctgcacc
     1621 acagcgacat tggcggttac accaccctgt ttgagatgaa gcgcagcaaa gagctgctgc
     1681 tgcgctggtg cgatttcagc gccttcacgc cgatgatgcg cacccacgaa ggtaaccgtc
     1741 ctggcgacaa ctggcagttt gacggcgacg cagaaactat cgcccatttt gcccgtatga
     1801 cctccgtctt caccaccctg aaaccttacc tgaaagaggc tgtcgcgctg aatgcgaagt
     1861 ccggcctgcc ggttatgcgc ccgctgttcc tgcattacga agacgatgcg cacacttaca
     1921 ccctgaaata tcagtacctg ttaggtcgcg acattctggt cgctccggtg catgaagaag
     1981 gccgtagcga ctggacgctc tatctgccgg aggataactg ggtccacgcc tggacgggtg
     2041 aagcgttccg gggcggggaa gttaccgtta atgcgcccat cggcaagccg ccggtctttt
     2101 atcgcgccga tagcgaatgg gcggcactgt tcgcgtcgtt aaaaagcatc taatcacatc
     2161 cgcccgtggg gaaactcatg ggcaaaggga gaacatcgat gagtcacatc acaacggaag
     2221 atccagcaac tttacgcctg ccctttaaag agaaactctc ttacggtatt ggcgacctgg
     2281 cctctaacat cctgctggat atcggtacgc tttatctttt gaagttttat accgacgttc
     2341 tggggctgcc aggcacctat ggcggcatta tctttttgat ttcaaaattc tttactgcgt
     2401 ttaccgatat gggtaccggc attatgttgg attcccgacg caagatcggt ccaaaaggta
     2461 agttccgtcc ttttattctg tatgcgtcat tcccggtcac cttactggcg atcgccaact
     2521 ttgtcggcac accgtttgat gtcaccggta aaacggtgat ggccactatt ctgtttatgc
     2581 tttacggact gtttttcagc atgatgaact gctcctacgg cgcaatggtt cccgctatca
     2641 ccaaaaaccc caacgaacgc gcctcactgg cggcatggcg tcagggcggc gctacgctgg
     2701 gcctgctgct gtgcacggtg ggattcgtgc cagtgatgaa tcttatcgag ggtaatcagc
     2761 aacttggcta tatcttcgcc gccacgctgt tttcactgtt cggcctgctg tttatgtgga
     2821 tctgctactc aggcgtgaaa gagcgttatg tcgaaaccca accaaccaat ccggcgcaaa
     2881 agcctggcct gttgcaatct ttccgcgcaa ttgccggtaa ccgcccactg ttcattctgt
     2941 gtattgccaa cctctgcacc ttaggggcgt ttaacgtcaa gctcgccatt caggtctatt
     3001 acacccagta cgtacttaac gatcccatcc tgttgtcgta tatgggattt ttcagcatgg
     3061 gctgtatttt catcggcgtg ttcctgatgc ctggcgcagt cagacgtttt ggtaagaaga
     3121 aggtctatat cggcggcctg ctgatttggg tgctgggcga tctgctcaac tatttcttcg
     3181 gcggcggctc ggtcagcttt gtggcgttct cctgcctggc gttcttcggc tcagcgtttg
     3241 ttaacagcct gaactgggcg ctggtttccg acaccgtcga gtacggcgag tggcgcaccg
     3301 gcgtgcgttc ggaaggcacg gtctacaccg gcttcacctt ctttcgcaaa gtgtctcagg
     3361 cgctggctgg tttcttcccc ggctggatgc tgacgcaaat tggctatgtg ccgaacgtcg
     3421 cacaggctga ccacaccatt gaagggttac gccagttgat cttcatctac ccaagcgcac
     3481 tggcggtagt caccattgtg gcgatgggtt gcttctacag cctgaacgag aagatgtatg
     3541 tccgcattgt ggaagagata gaagcccgta aacgcacggc gtaataataa taaacgacgc
     3601 cctgcggggc gttataagga gtgattatgt ctgaccataa tccactgaca ttaaaactga
     3661 atctgcggga aaaaatcgcc tatggtatgg gcgacgtcgg ttcgaattta atgctctgca
     3721 tcggtactct gtatctcctc aaattttata ccgatgaatt agggatgcct gcttactatg
     3781 gcgggattat ctttctggtg gcgaagtttt ttaccgcgtt taccgatatg ctcaccggat
     3841 ttttactcga ctcgcggaaa aatattgggc ctaaaggcaa atttcgtccc tttattttat
     3901 atgcagcagt tccggcggcg ttaattgcaa cgctccagtt tatcgccacc accttttgtt
     3961 tgccggttaa aacgacgatt gccaccgcgc tgtttatgat gtttggactt tcatacagtc
     4021 tgatgaactg ctcgtatggt gcgatgatcc cggcaattac caaaaacccg aatgagcgcg
     4081 cgcagctcgc ggcttaccgc cagggtggtg caaccatagg gctattgatt tgtaccgtgg
     4141 cgtttattcc gttgcagtcg cttttttctg actcaaccgt cggttatgcc tgtgcggcac
     4201 ttatgttctc cattggcggc tttattttta tgatgctgtg ctacagaggc gtcaaagagc
     4261 attatgtgga cacagcgcca accggacata aagccagtat tctcaaatct ttttgcgcga
     4321 tatttcgtaa tccaccattg ctggttttat gcattgctaa cctgtgtact ctggcggcat
     4381 ttaatatcaa actggcgatt caggtctatt acacccagta tgtgctgaat gatattaatt
     4441 tattgtcgtg gatgggattc ttcagtatgg gatgcatcct cgtcggcgta ttactggtgc
     4501 cagtaactgt aaaatgtttt ggtaaaaaac aggtttattt agctggcatg gtgctatggg
     4561 cggtgggtga tatactgaat tatttctggg gaagtaactc tttcactttc gtcatgttct
     4621 cttgtgtcgc cttttttggc acggcctttg taaacagcct gaactgggca ctggtaccag
     4681 ataccgtaga ttacggtgaa tggaaaaccg gtattcgtgc cgaaggttct gtttataccg
     4741 gttatacctt ctttcgtaaa atttctgccg cacttgctgg cttcttgcca ggcattatgc
     4801 tgacgcaaat tggttatgtt cccaacatcg cgcaaagcga tgcgacatta cagggtttgc
     4861 gtcagctcat ttttatctgg ccttgtgcac tggcaattat tgctgcattg acgatgggat
     4921 tcttttacac actcaatgaa aaacggtttg cattgattat tgaggaaatc aaccaacgca
     4981 aaaataaaga gatggcgaca gaagaaaaaa cggcttccgt aacgttataa atgtcaatag
     5041 ccgctatttc catcctggtg ggtggcggcc tccctacgtt taaaaaatgg acttattatg
     5101 aaaaagatta atgcaataat tttattatct tctctgacct ctgcctcggt atttgctggc
     5161 gcatacgtcg aaaacaggga ggcatataat cttgcctccg atcagggcga ggttatgcta
     5221 cgtgtgggtt ataacttcga tatgggcgcg ggtattatgt taaccaatac ctacaccttt
     5281 cagcgagaag atgaactaaa acatggctat aatgaaattg aaggttggta tcctttattc
     5341 aaaccaaccg ataaattaac catccagccc ggtggcttaa ttaatgataa gagtatcggt
     5401 tcgggtggtg cagtgtatct ggacgtcaac tataaatttg taccatggtt taatctgaca
     5461 gtacgaaatc gctataacca taataactat agttcaacag atttgagcgg ggaactggat
     5521 aataatgaca cctatgaaat tggcacctac tggaatttta aaatcaccga taaattttcc
     5581 tatacatttg agccacatta tttcatacgc gtaaatgact ttaatagcag caatggaaaa
     5641 gatcatcact gggaaatcac taataccttc cgttatcgca ttaatgaaca ctggctgcct
     5701 tatttcgaat tacgttggtt agatcgtaat gtcgaaccgt accaccgcga gcaaaaccag
     5761 attcgtatcg ggacgaagta tttcttctga tgtgaaactg ccggatgcgg cgtaaacgcc
     5821 ttatccggcc tacggttgat acggttttcg ttggccggat taagcacatc cggccattaa
     5881 tcaagcttca gccgccagca tctctttctt acgttggcta attcgcttaa ccagcattac
     5941 ggaaaccacc gcaccacaga atgcgaagca ggccataatg ccaaacacga ttttgtagcc
     6001 aataatcccc ggatttaaat ccagaatgta gccatacaga ctgaagcaga acatcgccgg
     6061 agcgtaacca ataaagctac ccaacgccat cgccgcgcct gttttatttt cagcaatttt
     6121 tgcttcgccg ataggtgcaa aaaatacggc tcgctgtgta aagactatcg cgccaaagcc
     6181 cagcgtacat gccatcccta aatagaccgg catactttcg tgcggcagca taatcaacag
     6241 tacgagcgca gcggtactga tgataaaggt gtagcataga tatttactcg gcgatttcag
     6301 gatcttatct gaaatcatgc caccaatcgg tccaccaatc attttcagac agtattggtt
     6361 aatgatgccg taagccccca ccagcgcaac gggcaataga tagatgtttt tcaggaatgg
     6421 aatgaagaat gtcaggccac agtaaaccgc gtaaacgaag aagacgttaa aagcgataag
     6481 ccagatggtt ttgtctctca gcaccgaggt catactggtg ttttgcgatg ctccgtcttc
     6541 ttttttcacc tcaacggacg gtgcctcttc tttgtcattc aggacaaaga aaataataat
     6601 gcctacggca atcacaataa gggaatagaa ccagatgcct gctttaaaac ctaataagcc
     6661 actgccaaac caggtaaata ctgccaacgc agaaaatgcc accacggtat cgacaatgcc
     6721 acgccctgtt tcgaagaagc caaacaaccg accttgttgt tcgctattgc ccaatcgact
     6781 taccgacttg agcaagaccg gccagttcat catgtcgcaa gtaacgccaa atagcgccca
     6841 gacaaagagg atgccccaat accccggcat tgtcgtcaaa taaacaccca gtaatcctgt
     6901 cgcaatgagt gagaatgaca tggtgtatct gcgcggtagt ttatcggcaa aataaataga
     6961 cagaaaaaag cccactgtgg tgacaaatga gtttaccgac atagcattac caatttgacc
     7021 attggtcaaa tggaaatatt cctgcatcgg gatataaaac gcatctttca gcgacggtaa
     7081 tttataaatt gtaccgccac acaatgttaa tagactaaat aacgcccatt tctttttcgt
     7141 gagcataaca tcccctaagc gatttttaaa atcactgttt taaaggcaat aagataccga
     7201 ccattcatat ggaatggttt tattaactgc gtatgagtta aaacttatta gtggttcaga
     7261 agatggtttg cctcttgttt gatttgttgt agtacgttac gcacatgatt tatctgatta
     7321 attgcatctt ctttttcttt acgtaaccgc gcatccagta aatgattttc tggcaatggt
     7381 gtttcactca tctggcgata agggatccac ggattatcat cttcgtcttc aaagcggaac
     7441 gccggcgcat agtaatcaac ctcttcttcc aggccatatg ccgtcacctg cggctcatca
     7501 tcacccaggc atataaggtg cgttaataac gctttgaacg gcatatcacc ctgacctgaa
     7561 atacaggctt tatgacccag gccacccggt tctttaacga tcaaggcatc tttgatatgg
     7621 acctgggtaa tatgcggcgc catcgttttt aaagcgtcga tgggatgctc atttgcattg
     7681 atcatgttcg caaaatcaaa cagtaaggaa agagattcca tctcactttc tttgaccagc
     7741 gacaccagct catggctttt taaatcttca tgctgttcga tcgtaaaagt caggccgctg
     7801 tcctgatacg tttcccgtac ataggcaatg tcgttagcga tgatcgataa tacgtcgcgc
     7861 aaattacctt cataacgtgg ataaaaacgt acggacgatg ccccagtttt caacgcaatg
     7921 gcgacggctt cgtcaataga tgccttatct gaagcgctgg tttcaatatg aatatcaagg
     7981 ttcagacggc gggctttatc cccaaaggca gagagttctt tatcgtccat attaccaaga
     8041 gaaaaacgct cgccatccag aacatggatt ttgacgccac gtagattatt ttcacttgcg
     8101 atatcaagta aatcagcagg caacactctt tccagccgca tatttaaatg aaaggcataa
     8161 gcatgtaaat aaagaggaag agtgtcgaca cgttgtagaa tctttcttgc gttatttatt
     8221 gtcaccataa aacacctaca gattaatgat gaggtctacg gcctcaaatc gccaatattc
     8281 aatatctatc tggacaatat tgccttgctg gtcggcgcga tgcttttcaa ttaagatcgc
     8341 tggcataccc gcagaaccgc caagatactt gctgatatcg cccggcattc tgacgggttt
     8401 gaaaatcaat ttttttacta ccgtctcttt ttggtagcac ttttcccaga ctgcagaaaa
     8461 tgagtggttt tcaagttgtt caataaaacc cggagcaact tcaggattaa tatatgtttc
     8521 gtgatagaaa actttatgtc cttccagcgc cccccagccg gtgatgcgat agagttcagt
     8581 tgatggtgtc actgcaatca gtggcgcgag cgtcttggga atatcgctgg tacggttttt
     8641 ctcggtaaac ccccaggaag gctctcgtcc ttgctcaatt gcggcccgct gaaagctcgc
     8701 cgacaactcc ggactgtaat taaatcgcgg ctgggtcacg aaccacccct tacgatcctt
     8761 acggaagatt ttggattctg cctcgaggtt caacaacgcc tggcgcagcg tcatacgttt
     8821 aatgcccagc agttctccca gctctctttc tgaagggagt tttccacccg gcgtggtgat
     8881 gccatctttc aaccaccgat ccagtttctc ttttgcattt tctacggtgg attgattctc
     8941 cgccattgtt taatcctttt ggtttaaacc agatgaagca tcttacgctt acgccaaaaa
     9001 agaaagcaat taacggcgta tgactttgca gataattgtg acaaagctct ctattgacgt
     9061 aatcccagac ttataaggga gatactcgca ttggtgtgcg ctcacgacag gtaaaaaaaa
     9121 acctgccagc gatggcaggt tttttataac taaaaaacga aattaatcgt ctttcggtgc
     9181 gcggttggcg cggcgacgat cgttttccgt caggtgacgt ttacgaatac ggatagaggt
     9241 cggagtcact tctaccagtt cgtcatcatc gatgaactcc agagcttgtt ccagagtcat
     9301 gcggataggc ggaaccagaa caacggcttc gtcagtaccg gaagcacgca tgttggtcag
     9361 tttcttaccg gtcaggcagt ttaccgtcag gtcgttagag cggctatgaa taccgataat
     9421 ctgaccttcg taaacttctg caccgtgacc gaggaacagc ttaccgcgat cctgcagacc
     9481 gaacagcgcg aacgcgaccg ctttaccctg accgttagag atcagtacgc cgttctgacg
     9541 ctgacccact tcacccggac gtacgtcgtc gtagtggctg aaggtggagt acagcagacc
     9601 agtaccggaa gtcatggtca tgaactcaga acggaagcca atcagaccac ggcttgggat
     9661 cacgtagtcg agacgtacgc ggcctttacc gtctggattc atgtttttca ggtcgccttt
     9721 acgttcgccc agcgcctgca ttacagaacc ctgatgctgt tcttcaacgt ccagagtcac
     9781 gttttcatac ggctcttgtt tacgaccgtc gatttcacgg aagataactt tcggacggga
     9841 taccgccagt tcgaaacctt cacgacgcat gttttcgatc agaacagaca ggtgcagttc
     9901 gccacgacca gaaacgcgga acgcatcggc gtcttcggtt tcttctacgc gcagcgcaac
     9961 gttgtgtacc agttctttgt tcagacgatc caggatctga cgagacgtta cgaacttacc
    10021 ttctttaccg cagaacggcg aggtgttaac gcagaagaac atagaaacgg tcggctcatc
    10081 aacggagagt gccggcagcg cttcaacgtt ttgcgtgtcg caaacggtgt cagaaatgtt
    10141 cagttcgcca aggcccgtga tcgcaacgat atcgccagct tccgccagat cggtttcgat
    10201 acgttccaga ccgaggtggc ccagcacttt accgactttc gcgttgcggg ttttgccttc
    10261 gctatcgatg atagtgacct gctggttcgg cttcacttta ccgcgcttga tgcggccaat
    10321 gccgataacg ccaacatagc tgttgtaatc gagctgagaa atctgcatct ggaacggacc
    10381 gtcaaggtca acgtccggcg caggaacgtg gtcaacaatc gcctggtaca gcggggtcat
    10441 gtcttccgcc atatcttcgt ggtccagacc cgcgataccg ttcagcgcag aagcgtaaac
    10501 gatcgggaag tccagctgct cgtcggtcgc gtcgaggtta acgaacagat cgaatacctg
    10561 atccacaacc caatcaggac gcgcgccagg gcggtcaact ttgttgataa caacaatcgg
    10621 cttcaggccg taagcaaacg cttttttggt tacgaagcgc gtttgcggca tcgggccgtc
    10681 aaatgcgtca accaccagca gcactgagtc taccatggac attacacgtt caacttcacc
    10741 accgaagtcg gcgtgccccg gggtatcaac gatgttgata cggtaatcat tccatttgat
    10801 agcggtgttt ttcgcgagga tggtaatccc ccgctctttc tccaaatcgt tggagtccat
    10861 cacgcgctct tgggtttcgg cacgagagtc gaacgtaccg gattgttgga gcagcttgtc
    10921 taccagggtg gttttaccat ggtctacgtg cgcgatgatg gcgatattac gcaatttttc
    10981 gatcacaact ttgcctcagg cattagaaat agcgcgttat tgtacacgga ttaatcgcac
    11041 tacaaaacag gatcacaaac atcctccgca aacaagtatt gcagagtccc tttgtgatcg
    11101 ctttcacgga gcataaaaag ggttatccaa aggtcattgc accaacatgg tgcttaatgt
    11161 ttccattgaa gcactatatt ggtgcaacat tcacatcgtg gtgcagccct tttgcacgat
    11221 ggtgcgcatg ataacgcctt ttaggggcaa tttaaaagtt ggcacagatt tcgctttatc
    11281 ttttttacgg cgacacggcc aaaataattg cagatttcgt taccacgacg accatgacca
    11341 atccaggaga gttaaagtat gtccgctgaa cacgtactga cgatgctgaa cgagcacgaa
    11401 gtgaagtttg ttgatttgcg cttcaccgat actaaaggta aagaacagca cgtcactatc
    11461 cctgctcatc aggtgaatgc tgaattcttc gaagaaggca aaatgtttga cggctcctcg
    11521 attggcggct ggaaaggcat taacgagtcc gacatggtgc tgatgccaga cgcatccacc
    11581 gcagtgattg acccgttctt cgccgactcc accctgatta tccgttgcga catccttgaa
    11641 cctggcaccc tgcaaggcta tgaccgtgac ccgcgctcca ttgcgaagcg cgccgaagat
    11701 tacctgcgtt ccactggcat tgccgacacc gtactgttcg ggccagaacc tgaattcttc
    11761 ctgttcgatg acatccgttt cggatcatct atctccggtt cccacgttgc tatcgacgat
    11821 atcgaaggcg catggaactc ctccacccaa tacgaaggtg gtaacaaagg tcaccgtccg
    11881 gcagtgaaag gcggttactt cccggttcca ccggtagact cggctcagga tattcgttct
    11941 gaaatgtgtc tggtgatgga acagatgggt ctggtggttg aagcccatca ccacgaagta
    12001 gcgactgctg gtcagaacga agtggctacc cgcttcaata ccatgaccaa aaaagctgac
    12061 gaaattcaga tctacaaata tgttgtgcac aacgtagcgc accgcttcgg taaaaccgcg
    12121 acctttatgc caaaaccgat gttcggtgat aacggctccg gtatgcactg ccacatgtct
    12181 ctgtctaaaa acggcgttaa cctgttcgca ggcgacaaat acgcaggtct gtctgagcag
    12241 gcgctgtact acattggcgg cgtaatcaaa cacgctaaag cgattaacgc cctggcaaac
    12301 ccgaccacca actcttataa gcgtctggtc ccgggctatg aagcaccggt aatgctggct
    12361 tactctgcgc gtaaccgttc tgcgtctatc cgtattccgg tggtttcttc tccgaaagca
    12421 cgtcgtatcg aagtacgttt cccggatccg gcagctaacc cgtacctgtg ctttgctgcc
    12481 ctgctgatgg ccggtcttga tggtatcaag aacaagatcc atccgggcga agccatggac
    12541 aaaaacctgt atgacctgcc gccagaagaa gcgaaagaga tcccacaggt tgcaggctct
    12601 ctggaagaag cactgaacga actggatctg gaccgcgagt tcctgaaagc cggtggcgtg
    12661 ttcactgacg aagcaattga tgcgtacatc gctctgcgtc gcgaagaaga tgaccgcgtg
    12721 cgtatgactc cgcatccggt agagtttgag ctgtactaca gcgtctaata gttgaagttg
    12781 tactacccgg cgcaacaacg ccggaggttc aaaccaggcc catctggcgt aattgttgca
    12841 gccagtttga acacggacag cgcgcagaac ccggagcgta cactggtacg tgaggagttc
    12901 gagcactgcc caggttcaaa atggcaaata aaatagcctg atgggactgg tttttagttg
    12961 ccgtggaaat ttcagcccat ctctgcatgg gcttttttct ccgtcaattc tctgatgctt
    13021 cgcgcttttt atccgtaaaa agctataatg cactaaaatg gtgcaacctg ttcaggagac
    13081 tgctttatgg caacaggcac gcagcccgat gctgggcaga tcctcaactc gctgattaac
    13141 agtattttgt taatcgatga caacctggcg atccattacg ccaaccctgc cgcgcaacaa
    13201 ctgctcgccc aaagctcccg caaattgttt ggtacaccgt taccggaact gttgagctac
    13261 ttctcattaa atatcgagct gatgcaagaa agtctggagg cggggcaagg ttttaccgat
    13321 aacgaagtga cgctggtcat cgacgggcgc tcgcatatcc tttctgtgac ggcccagcgt
    13381 atgccggacg gcatgatcct gctggagatg gctccgatgg ataaccagcg ccgcttaagt
    13441 caggaacagc tacagcacgc ccagcaggtt gctgcccgtg atttagtgcg cggcctggca
    13501 catgagatta aaaatccgct tggcggttta cgtggcgcgg cgcagctgct cagcaaagcg
    13561 ttacctgacc catcactact cgaatatacc aaagtgatta tcgaacaggc ggaccggctg
    13621 cgaaatctgg tcgaccgtct gttggggccg cagctgcccg gtacgcgcgt taccgaaagt
    13681 attcacaaag tggctgaacg cgtggtaacg ctggtgtcga tggaactgcc ggacaacgtg
    13741 cggttgattc gtgattacga tcccagcctg ccggaactgg cgcacgaccc ggatcaaatt
    13801 gaacaggtct tgctgaatat tgtgcgcaat gcgctacagg cgctggggcc ggaaggcggt
    13861 gaaatcattc tgcgtacccg caccgcgttt caactgacct tacacggcga gcgctaccgg
    13921 ctggcggcgc ggattgatgt ggaagataac gggccgggca ttccgcctca tttgcaggat
    13981 acgctgtttt acccgatggt cagcggccgc gaaggtggca ccgggcttgg cttatccatc
    14041 gctcgtaatt tgattgatca gcattcaggc aaaattgaat ttaccagttg gccagggcat
    14101 accgagttct cggtttacct gcctatcagg aaataaaggt gacgtttatg caacgaggga
    14161 tagtctgggt agtcgatgac gatagttcca tccgttgggt gcttgaacgt gcgctcgctg
    14221 gggcaggttt aacctgtacg acgtttgaga acggcgcaga agtgctggag gcgctggcga
    14281 gcaaaacgcc ggatgtgctg ctttcagata tccgtatgcc gggaatggac gggctggcgc
    14341 tgctcaagca gattaaacag cgccatccaa tgcttccggt catcattatg accgcacatt
    14401 ccgatctgga tgctgccgtc agcgcctatc aacaaggggc gtttgattat ctgcccaaac
    14461 cgtttgatat cgacgaagca gtggcgctgg ttgagcgcgc tatcagtcat taccaggaac
    14521 agcagcagcc gcgtaatgtt cagcttaacg gcccaacgac cgatatcatc ggcgaagcgc
    14581 cagccatgca ggacgtgttc cgtattatcg gtcggctttc gcgttcttct attagcgtgc
    14641 tgattaacgg cgaatccggc accggtaaag aactggtcgc tcatgccctg catcgccaca
    14701 gtccgcgcgc caaagcgccg tttatcgcgc tgaatatggc agctatccca aaagatttga
    14761 tcgaatcaga actgtttggc cacgagaaag gcgcgtttac tggcgcgaat accattcgtc
    14821 aggggcgttt tgaacaggcc gatggcggta cattattcct cgacgaaatt ggtgatatgc
    14881 cgctggatgt gcagacgcgt ttgctgcgcg tgctggcaga cggtcagttt taccgcgttg
    14941 gcggctatgc gccggtgaaa gtggatgtgc ggattatcgc tgccactcac cagaatctcg
    15001 aacagcgagt gcaggaaggt aagttccgtg aggatctgtt ccaccgcctg aacgttatcc
    15061 gcgttcatct gccgccgctg cgcgaacgtc gggaagatat tccccgtctg gcgcgccatt
    15121 ttttacaggt tgccgcgcgc gaactgggcg tagaagcgaa gttactgcat ccggaaaccg
    15181 aagctgctct gacgcgtctg gcgtggccag gcaacgtgcg ccagctggaa aacacctgcc
    15241 gctggctaac ggtgatggcc gccgggcagg aagtgttgat tcaggatttg cccggcgaac
    15301 tgtttgaatc aacggttgcg gagagtactt cgcaaatgca accggacagc tgggcgacgc
    15361 ttcttgcgca gtgggcagac agagcgctgc gttccggtca tcaaaatctg ctttccgaag
    15421 cgcagccaga gctggagcgg acgttactga cgaccgcgtt gcgacatacg caggggcata
    15481 aacaggaagc ggcgcggcta ctcggctggg gccgcaacac cctgacgcgt aagttaaaag
    15541 agctggggat ggagtgattc acagcttgtg tgtaaagatt gattattgag cgcaaattgc
    15601 tggtatttta cgctttactg ttccgataag ttcagtatga tcttgcccgg aaaacgggga
    15661 gagtcattat gctggaatca ataattaatc tggtatcgag tggcgcagtt gacagccaca
    15721 caccgcaaac tgccgttgct gccgtgctgt gtgccgcaat gattgggctg tttagttgag
    15781 tttcagattg ctgatgaagt gctctttgtt catgccggat gcggcgtgaa cgccttatcc
    15841 ggcctacaaa agcaatattt tcaacaggtt ttgtaggcct gataagcgta gcgcatcagg
    15901 caatcttgtt ctcagcacat tgatggcgct ttcgttttct actttgtaaa cgaagcgcca
    15961 ttcactattt aaatcacccg tgagaactgc tgcatccgcg ctttctggcg cagataggta
    16021 tcaaagcaca tgcaaatgtt gcggatcagc aagcgacctt tcgccgtcac ctgtattccc
    16081 ttctcatcca catccaccag cccatctttt gctaacgggg cgagcagctt gagatcttcc
    16141 gcaaagtaat cagcgaagtg caaatcccac agtttctcaa taggggcgta atccagacgg
    16201 aagttgcaga tgagcgactt aatcacatcg cggcgaatac agtcatcacg cgttagcgca
    16261 ataccacgcc acagcgcatt gccttgttca tccacttgct gatagtactg cttcaactct
    16321 ttctggttct gcgcgtagca gtcgccaatc atgctgatgg cggaaacgcc catccccagc
    16381 agatcggtat cgccctgagt ggtgtagccc tggaagttac gatgcagcac gccttcacgc
    16441 tgggccaccg ccagctcgtc atccggacgg gcaaagtgat ccataccgat aaactgatag
    16501 cccgattgcg tcaggaaggc gatggtttcc tgcaggatat cgagtttttg ctgcggactc
    16561 ggcaggtcag catctttgat tttgcgctga gcagcaaaaa tggtcggcag atgcgcgtag
    16621 ttaaagacac tcagacgatc ggggttcagc tccgccacac gtttcagggt aaaggcgaaa
    16681 ctctccggcg tctgtttcgg caggccgtaa atcaggtcga tgttggtgga ggtaaaacca
    16741 atctcacgcg catggttaag cagtgcaaag atgaactctt catcctgctc gcggttaacc
    16801 agacgttgca cttctttgtt gaagtcctgc acgcccatgc tcaggcgatt aaagccttcg
    16861 gcgcgtaaat gatcgagtac atccagttcg atttcccgcg gatcgacttc gatcgaaatc
    16921 tccgcatcgg cattgaactg gaagttttcg cgcagcagct tcatcaggcg gctgatttgc
    16981 gctttattca gatacgtcgg cgttccgccg ccccagtgca attggctgac gtgacgcccg
    17041 gcgaacagcg gtgcacgatg gacgatttct tgctccagcg cgtccagata ctgatcggcc
    17101 ttgtgctgct ggcgagtaac aatcttattg caaccgcaga agtaacaaag cttatggcag
    17161 aacgggatat gtacgtagag agataatgga cgctcaggat agcgcgccac ggcttgtaaa
    17221 aacgcctgtt cgccgaagtc ttctgaaaac tccagcgcgg tcgggtacga ggtgtatcgt
    17281 ggcccggaat agttatattt ctggatcagg gccagatccc agtcgatttg ctgtacagac
    17341 atgctcactc cttccgatgg cgtctctggc ggctacggcg taccggcgga tgcccgtttc
    17401 gggtcatcag ccggttgcgc agccactttt gtcgccgcga caaccgctgt agtttaacga
    17461 ataaccacac cagataacat ataaccggaa gggttatcag cagaacggga aggcccacgg
    17521 cggcaatccg ttagttgccc cgcagcagac gcatcatgtc ttcttgcttc tcgtcttctt
    17581 cctcttcttc gtcatcgtca taagagaggc cgagtttctg catcaactca tcaatacgat
    17641 ccagtttagc atccacccag gattgctctt cggcgctcag ggtttcgcct gcttccagac
    17701 gttccagcag cgcatccaga cgctcatccg tttccagtaa ttccaactcc gcctgcggtg
    17761 aaagcatagg tttctcactc ttcggtttgt gctgtttggt gactttttca gtcacgccca
    17821 atggaatagg ggttttactg ccaatacgtg gatcttttgg tgcgttctgg cctttgctgc
    17881 ctgacgtggt gtttccgccc gctgcgcggc tgcccggcgc atgaccacga cgttttttct
    17941 gacgcttgcg atcacgagct tcctgatcca gctcctcgcg tgtttttcga cgcgcttttg
    18001 catgaccttt gctgcgtgag tttgaagatg atggtttcat aatggttcgt cttttagccg
    18061 ttttattcag tatagatttg cggcggaatc taacagaaag caagcaaaga aaaaaggcga
    18121 cagattactc tgtcgccttt tttcctgact cataaccctt aacgggtctt acaatccttg
    18181 caggccgcca acacaccctg tttttccttt agccggaaca atccgtgttg attccatttc
    18241 cgtttaatta cgtctccgga cgtttgtctt cctgacaatc ctctgtcttc gcctcctggc
    18301 gctcctgacc ctcatcctga gtcattgttc ctgttagcgt cctcgctcta tgggtgctac
    18361 tttacgcctt tgctttaaac aaacaatcaa ccgccatcag tatttgcaca ttttcagagt
    18421 tttatataaa agcaattcac tgatttatat agcttatttg acatgcatcg cagaattttc
    18481 tctgctattt cgcataaaga ctatcggcaa tgtctcacaa agcgtatggc ttttacttac
    18541 aacgctcacg ccgaaaaatc cgccttttat tatggttcag gtataatcgc cacaaagctt
    18601 tgactgacgg agacgaccgc tttgactaat ttgaattatc aacagacgca ttttgtgatg
    18661 agtgcgcctg atattcgcca cctaccttcc gataccggaa ttgaagtggc ttttgcaggc
    18721 cgttccaacg caggtaaatc cagcgcgctg aacacgctga ctaaccagaa aagcctggct
    18781 cgtacctcaa aaaccccagg gcgcacccag cttatcaacc tgtttgaagt ggctgacggc
    18841 aagcgtctgg ttgacttgcc tgggtacggt tatgcggaag tcccggaaga gatgaagcgc
    18901 aaatggcagc gtgcgctcgg cgaatacctc gaaaaacgtc agagcctgca aggtctggtg
    18961 gtgctaatgg atattcgcca tccgctgaaa gatttggatc agcagatgat tgagtgggcg
    19021 gtagacagca atatcgccgt tctggtgctg ctgaccaaag cggacaaact ggcaagcggc
    19081 gcacgtaaag cgcaattgaa tatggtgcgt gaagctgtac tggcgtttaa cggtgatgtg
    19141 caggttgaaa cgttttcttc gttgaagaaa caaggcgtgg acaagctgcg gcagaaactg
    19201 gatacctggt ttagcgagat gcagcctgta gaagaaacgc aggacggcga ataattttct
    19261 tgccttaatg cttgtgccgg atgtggcgta tccggcccgt aaattcaata gattacaatt
    19321 ctaccgttgg cctgataaac atagcgcatc aggcattacg gatcttttct ttcgcccaat
    19381 aaaaaacgcc ccagtcatta ctgactgggg cggctaaaat attcagccaa atccgattac
    19441 gtgaagtaaa aggtctgaaa gatagaacat cttacctctg taccctacgc gactaacttt
    19501 actctttttt gttcagttca gaaagcactt tttgtaattt tttttcagtt gttgcatagg
    19561 aatttctaat agccatcaca aaacgtgaca gcttatgttg cttacttacg aaaaaagggc
    19621 atgttcaggc gaatcttagt gcgcctgatc ccagttttcg ccactcccca cttccaccag
    19681 caacggcaca tccagacggg tacagttttc catcagttga tgaatctgct tcgcgacggc
    19741 atcaacatca tctttatgaa cttcaaatac cagttcatcg tgtacctgca tgatcatacg
    19801 tacacgcggt tgctcagcct gtaaccacgc atcaacggca atcatcgccc gtttgataat
    19861 gtcggcggcg gttccctgca ttggcgcgtt aatggctgca cgttcagccg ctgcacgacg
    19921 agcaccattg ctggatttga tatccggcag atacagacgg cgtccgtcca gcgtttcaac
    19981 gtagccctgc tctttcgcct gagcacgggt gcgttccata tactccagca cgccagggta
    20041 gcgttcgaag taaaggtcca tgtacttctg cgcttcttta cgtggaatgt tcaattgccg
    20101 cgccagaccg aaagcactca tgccataaat cagaccaaag ttgatcgctt tcgcgctacg
    20161 gcgttgctcg ctggtgacgg tttccagtgg caaactaaac acttctgccg ccgttgcccg
    20221 gtggatatct tttccttccg cgaatgcggt cagcaagcct ttgtcacgcg aaagatgcgc
    20281 cataatgcgc agttcaatct gcgagtagtc cgctgagaca atcacataat cctctggcgc
    20341 aataaacgcc tggcggatac gacgaccttc ttcgttacgc accggaatgt tttgcaggtt
    20401 aggatcggtt gacgataaac gtcccgttgc agttactgcc tggtgataag aggtatgcac
    20461 acgcccggtt ttcgggttga tcatcagcgg cagcttgtcg gtgtaggtcg atttcagctt
    20521 cgccagacca cgatactcca gaatcacttt tggcaacgga tagtccagcg ccagttcttc
    20581 cagtacctct tccgacgttg acggcgcgcc acccggcgtt ttcttcagcg gtttaatacc
    20641 ctgtttttca aagagaatgg tttgcaactg cttggtggaa gaaaggttaa attcttcacc
    20701 tgcaatttca tgcgctttct tttccagctc agccagacgc aaggtgagct cttcagaatg
    20761 attgtgcagc actttcggat cgatcttcac accgttacgt tcaatgcgtg aaagcaccgg
    20821 caccagcggc atttcgatat tctcgaagac gttcaacggc cctttgtgtt tttgcagatc
    20881 tggccacatt ttcagatgca actgcaaggt gacatcagca tcttcggcgg cgtaacgtcc
    20941 ggcctcttcg agggcaatct ggttaaaggt cagttgattt ttgcctttac ccgcaatctc
    21001 ttcaaaagtg atggttttgt gcttcaacca acgttccgcg aggctgtcca tatcgtgacg
    21061 cccggcaacg ctattgagaa tgtaggactc cagcatggta tcaaacgcaa tcccacgcag
    21121 ctcaatgccg taattcgcca gaataccgcg atcgtatttc aggttttgcc cgaccttcag
    21181 cgccttttca tcttccagca gcggttttag caactcgagt gcacgctcgc gagagatttg
    21241 atcgggcgcg tcaagataat catgagcaac cggaatatat gccgctacgc ctggctcgat
    21301 agcaaaagaa agcccgacca ggttagcaga gatgttatca aggctgtcgg tttcggtatc
    21361 aaatgcaaat accggcgctt tttccagctt cgcaatccac gctttcagtg tttcttcatc
    21421 aaggatggtg acgtagttgt cataagaaat caccgttgcc gtcacttctg gcgcttcgtc
    21481 tgcaacactg gtttcctgcg gcttcgcggc tggttttgcc cctttggcct gtaaccattt
    21541 gcccgcttcg acatcagcag tccagcgttt gaactcatac tttttgaaca gccccaacaa
    21601 ctcttccgct gccggttgct gcacttccag ttgttcacag gtcagctcca gttcaacgtc
    21661 ggttttaatc gtcgccagct ggtatgagag ataagcaact tctttgtttt gctcgagctt
    21721 cgctgccatt gttttcgcgc cacggaagct caacccagca attttttctg gctcggcata
    21781 cagcgtatcc agcccgccaa gaccttgcag caatgcctgc gcggtttttt caccgacgcc
    21841 cggtacgcca ggaatgttat cggaggagtc acccatcagc gccaggaaat cgatgatcag
    21901 ttctggcggc acgccgtact tattcaccac ctcttccggt ccgaggatgg tattcgtcat
    21961 ggtattgata agcgtaatat ttggcgtcac cagctgcgcc atatctttat cgccagtgct
    22021 gatcagcacc ggacgcccgg ctttttcggc ttcgcgcgcc agagtaccga taacgtcgtc
    22081 cgcttctacg ccagaaaccg ccagcagcgg cagtcccatc gctttaacca tcgcgtgcag
    22141 gggttcgatt tgtgctcgca gatcgttcgg cattggcggg cgatgtgatt tgtaatgttc
    22201 aaacagttca tcacgaaagg tttttccctt ggcgtcaaag accaccgctg catgcgtcgg
    22261 tttatattgc atgatcagac tgcgcagcat gttgaggaca ccatacatcg caccggtcgg
    22321 ctcgcctgcg ctgttagtca gcgggggaaa cgcgtgatat gcgcgataaa gataagatga
    22381 accatctaca aggataagtg gattttgggg gatctgaacc ataatgtccg tgcctgttta
    22441 tcagattatg ggtaaaggat gccacagaca ggatgaaaac atgagttttt caccgctttt
    22501 cgtgaaaatc ttttgtagat cttctggatc gctcgcaaat cgtcatgtgg ataactttgt
    22561 gcataaatta tttacatcgc ttttaattta ttatcatgct ttttacagca tgatatttgt
    22621 ctttgttttt catgcagtta aaaataaagc catccctaat aattgagata atgggatttt
    22681 ttctaatgtg gatagatatg aattattttt ctccttaagg atcatccgtt atttgggtcg
    22741 ttttctgcta aaatctgcgc cttgcgcttc tttagcgcac tgtctatggc taaccttttg
    22801 aataaattca ttatgacgag aatactcgct gcgataaccc ttttgctgag tatcgtattg
    22861 accattttgg tcactatttt ttgttccgtc ccgatcatca ttgccgggat tgtaaaacta
    22921 ttgctacctg tgccagtcat ttggcgaaag gtttcgcgtt tttgtgattt tatgatgtat
    22981 tgctggtgtg aaggtctggc ggtattactg catctgaacc cacacctgca atgggaagtt
    23041 cacgggctgg aagggttaag taagaagaac tggtatctgc ttatttgtaa ccaccgtagt
    23101 tgggcagata ttgtcgtact gtgcgtgctg tttcgtaagc acattccgat gaataaatat
    23161 ttcctcaagc aacagctggc ctgggtgcca tttcttggcc tggcgtgctg ggcgctggat
    23221 atgccgttta tgaagcgtta ttcccgcgct tatttattac gtcatccgga gcgacgtggt
    23281 aaagatgttg aaaccactcg gcgttcttgt gaaaagtttc gcctgcatcc caccaccatt
    23341 gtcaatttcg ttgaaggctc ccgtttcacg caagaaaaac atcagcaaac ccactccact
    23401 tttcaaaact tgttgccacc aaaggcggca ggcattgcga tggcgctaaa tgtactgggt
    23461 aaacaattcg ataaactgct taatgttacg ctgtgttatc cggacaataa ccgtcagccg
    23521 ttcttcgata tgttaagcgg taaactgacg cgaattgtcg ttcatgtgga tttacagcct
    23581 attgccgatg agttacatgg cgactacatc aatgacaaaa gctttaagcg ccattttcag
    23641 caatggctga actcactgtg gcaggaaaaa gaccaacttc tgacgtcgtt gatgtcatca
    23701 cagcggcagg acaagtaaat tttgctcgcc tctgaatgag cagaggcgag tgagtatttt
    23761 aatgaacaaa acgtccggca cgagacataa attcttcttc gctcatctcc tgtccgttaa
    23821 aaacaacttt acccggtgta taacgcagtt gcagcgaggc ggtattgttg tcaatcgtga
    23881 ttaactgaaa catctgacct aaggtcatca tcccgctgat ttgtttatca gcccgctttt
    23941 gcgctttttc cgcatccatt ccttcagata aattaagctg ttttgcggtt tctgtgacga
    24001 cattaagcgg taacttcaca tcaaaattga gcgatttaat atctttgttt gtggatgatg
    24061 aagatttggc tgggtcggca atactgatat ccagattggc atttagttcg ccgagtgcgt
    24121 tcttccattt taccggttgt ttaatggtcg gctcactttt ttgtaataac ggtaaatact
    24181 ctttgaacaa tgcagcattc acttcttgca gagcaacttc atcgtttgct aactcagggt
    24241 gtgcagcaag ctgcttctgc attgcaatgt tatactggat gataaattgg cgcaccgctg
    24301 agggatcaat agattcagca atcaatgacc actcaccact accgagttgc tgattccctt
    24361 ttgttaactt atcaagttcg taggtcagat tgacattgat ataatctttg tctttaacgc
    24421 gatccagcat ggtttttgca acgaaagcat cgatttgggc aacgtcttcc ccgtgattga
    24481 taatgttaat ctgattaaat ttcgactcgc tttcaccaac cggaaatttc ttttcttcta
    24541 atcgcgccag agaatcaatg gtaaaacttt ttgccgttac tttgttcatt ggtgataatt
    24601 gcagaacaat atcttcgact ttgccttcaa tatccaggtt agacaatgaa ctaccatcac
    24661 cattaaaagt aaactggccc tcaccaaaag aaaactttcc atactctgca ggggcaacat
    24721 ctaatgtcgt agagaatgag ccaccaaacg cgaatcgcaa tgtcgcttcc acgggcgatt
    24781 tatttttcgc ggcgataaat agcggttgtg tcagttcatt attcactaag ttcacttttg
    24841 ccgctgccag tgctgggatg acattaccat gcattaacat tgtgatgggc aacggaccgt
    24901 gctcaacatc cacatcaaat acaactttct ggcctggctt gatattgaga tcgggtgcac
    24961 cgttatcgaa tgtcatttgc atttgaaaac gggaattaaa aaagcctttg tcaaagtttt
    25021 tataacttaa cgtcatatcg taggcatgtt cacctttctt tttcgcatcg ttaaaatctt
    25081 taataaactt ttcgatacca ggctgaattt gcgtacctgt gtaccatgtg ccaccacccc
    25141 agattacggc taacgcaaca ataacacctg tagctgactt acgtatcata taaatattcc
    25201 ctgataaaac agcaaaactt tgaatatcca cttatgctga aataatgtcc agcggcagga
    25261 tgcattatca gaatgtattt atatttatta aataataaaa aaagcccgtg aatattcacg
    25321 ggccttatgt aatttacatt gaattatttt ttctcggaca gatatttcac tgtatcagca
    25381 tactgctgaa caaaaacatc catattgctg gtatccatac cctgcggatt cagctgatat
    25441 ttaccgttaa caaacatcgc cggaacgcca cgcaattgca cgtcagctgc agctttttcc
    25501 tgctgagcga ccagagattt gaccacgaag ctgttccacg ccgcgtcgta ctcttcacct
    25561 ttaatacctg cgttgataaa tacatcgcgg atatcagatg ctgaacgaat ggtctgggtt
    25621 ttctgtacgc cttcaaacag cggaacagtg actttgtctt ccacgcccag cgccatcgcc
    25681 acagcccatg cctgagtcag atctttgccc aggtcaccac ccatgaagtt gacgtggtat
    25741 ttagtcatct tcacgccttc cggcagtttt ttcttcacat tatcagaaat atgcagaact
    25801 tcttcaaact gatagcagtg cggacagaag aaagagaaaa actccagcac ttgcggcgcg
    25861 ccagctaccg gtttttccag ggtagtgtac tgtttaccat cttcatactg cgccgccgat
    25921 gcgctaaacg ctaaaactaa accagccagc gccagccaaa tctttttcat gatctactct
    25981 ctccgattaa tacataggtg ttaattgtaa agggggttct tgtagaactt ttgcctgttc
    26041 tataaaagtc gccgtttgtc gcagccagta atcttccccg gttaaccacg ggaaattttt
    26101 cgggaacgcg ggatcagccc aacgccgcat tagccaggca agataataaa ccaaacgcat
    26161 ggcgcgtaaa ggttcaatca gtccgatttc agcggtgtcg aactcgctaa attcttcata
    26221 agcttcaata atagtttcca gttgcatccg ctgctcggct ttatcgccat tgagcaacat
    26281 ccacaaatcc tgaatagctg gaccattacg tgcatcatcc agatcaacaa acattggacc
    26341 atcgcgccag agaatattcc cggcgtggca gtctccatgt agccgcagaa cggtgaaatc
    26401 ttcccgccag tgcgctgtaa cggcggcgat cagctcatct gtcgctttca ggaatgccgc
    26461 tttcaacccg gaaggtatca gtgtagcgtc ctcaaacagc ttgcgtggct caatgagata
    26521 ttcattcaga ccgatggtcg ggcgatggat aaaaagctgt ttgcgccccg tctggtgcat
    26581 acgccctaaa taacgcccaa ccgcttccat ctgatcgata ttatcagctt cgaactggcg
    26641 accaccgacg cttggaaaaa cagcgaaata aaatccctga tgattcaata aagtctgacc
    26701 gttaaaggcc acaggtgctg cgaccggaac ttcatcattt accaactgca acgcaaattg
    26761 atgttcttcg aggatttgat cggctgtcca acgttcaggg cgataaaatt tgacgacaaa
    26821 acgtcgacga tcttcgtcct gaaattgata gacacggttt tcatagctgt taagcggggt
    26881 aagaccggaa tccacccgga tcccttgctc aaatagagca tccatgatgg tatccgggtg
    26941 tagtgtctgg aaagtaaaag cgctgttgtt catcccatca tccggaaaac attacgaatg
    27001 atgaaggata tcatttcacg atgattttgg tggcaccgct tacaagcttt tactctttaa
    27061 ttacgccgcg agcgcgtaac agcgccgttt tgaagtcttc ttcataatct ttctgcaaac
    27121 ccgggatcac cgcatctttc gcggaatcac gcattttcag gtgatagatc agaatgtcat
    27181 ccgtcaaatc cgccagttcg ccgtcaaaac ctgactcttt cgccagtttc tgcaaaaatt
    27241 gcaacaggtt aaggtctggc tctttttgcc aggctggctg gaggagttca ataacttcat
    27301 tcagacgttt acatttcata gtaatgctcc ttactcttga gacagacacg ttagcagggt
    27361 caatcccaca ataaaagagg cgatatcggt gaatctgatg acgacgataa caggcgttgt
    27421 gctggcaggc ggtaaagcca gacgaatggg cggcgtagat aaaggattgc ttgaattaaa
    27481 cggcaaacca ttatggcaac atgtcgctga cgcgcttatg acgcagctct ctcacgtcgt
    27541 ggttaatgcc aatcgccatc aggaaatcta tcaggcaagc ggtctgaaag tgattgaaga
    27601 ttcactggcg gattacccag gccctctggc aggaatgctt tcagtaatgc agcaggaagc
    27661 aggcgagtgg tttttgtttt gtccgtgcga tactccttat attcccgagg atttagcagc
    27721 ccggcttaat catcagcgca aagatgcgcc tgtcgtgtgg gtccacgacg gtgaacgcga
    27781 tcacccgact attgctctgg taaaccgcgc aattgagcct ttattactgg aatatctgca
    27841 agcaggagaa cgccgggtaa tggcatttat gcgtctggct ggcggtcatg cggttgattt
    27901 cagcgataat aaagatgcat ttattaacgt gaatacgcca gaggagcttg cccgatggca
    27961 ggaaaaacga tgataccgtt actcgccttt gccgcgtgga gtggtaccgg aaaaactacg
    28021 cttttgaaac aactgatccc ggcattatgc gccagaggga tccgtccagg gctgattaag
    28081 catacgcacc atgatatgga tgttgataag ccaggcaaag atagctatga gctgcgcaag
    28141 gctggcgcgg cacaaaccat cgttgccagc cagcaacgat gggccttgat gacggaaaca
    28201 ccagacgaag aagagctgga tctacagttt ctcgcaagtc gaatggatac ctcaaagctg
    28261 gatttgattc tggtcgaagg gtttaagcat gaagagatcg caaagattgt gctgtttcgc
    28321 gatggagccg gacatcgacc tgaagaatta gtgatagaca ggcatgttat tgctgtagcc
    28381 agtgatgtgc cgcttaatct tgatgtcgca ttactggata tcaatgatgt tgaggggctg
    28441 gctgattttg tggtggagtg gatgcaaaag cagaacggat aagcaagagt aggctggata
    28501 agacgcgcca gcgtcgcatc cggcattttt ttctgcgcca atgcaaaaag gccatcctga
    28561 cggatggcct tctgctt
//
LOCUS       DAZGJW010000056        28572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZGJW010000056 DAZGJW010000000
VERSION     DAZGJW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.056"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(70..1173)
                     /gene="gldA"
                     /locus_tag="ACHKIK_002393"
     CDS             complement(70..1173)
                     /gene="gldA"
                     /locus_tag="ACHKIK_002393"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO9083554.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(1184..1846)
                     /gene="fsa"
                     /locus_tag="ACHKIK_002394"
     CDS             complement(1184..1846)
                     /gene="fsa"
                     /locus_tag="ACHKIK_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO9083555.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAISDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(1858..4359)
                     /gene="ptsP"
                     /locus_tag="ACHKIK_002395"
                     /pseudo
     CDS             complement(1858..4359)
                     /gene="ptsP"
                     /locus_tag="ACHKIK_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            4668..5747
                     /gene="frwC"
                     /locus_tag="ACHKIK_002396"
     CDS             4668..5747
                     /gene="frwC"
                     /locus_tag="ACHKIK_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO9083556.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            5762..6082
                     /gene="frwB"
                     /locus_tag="ACHKIK_002397"
     CDS             5762..6082
                     /gene="frwB"
                     /locus_tag="ACHKIK_002397"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO9083557.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            6133..8430
                     /gene="pflD"
                     /locus_tag="ACHKIK_002398"
     CDS             6133..8430
                     /gene="pflD"
                     /locus_tag="ACHKIK_002398"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO9083558.1"
                     /translation="MTNRISRLKTALFSNTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            8396..9274
                     /gene="pflC"
                     /locus_tag="ACHKIK_002399"
     CDS             8396..9274
                     /gene="pflC"
                     /locus_tag="ACHKIK_002399"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO9083559.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDVPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            9276..9617
                     /gene="frwD"
                     /locus_tag="ACHKIK_002400"
     CDS             9276..9617
                     /gene="frwD"
                     /locus_tag="ACHKIK_002400"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO9083560.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(9604..10455)
                     /gene="yijO"
                     /locus_tag="ACHKIK_002401"
     CDS             complement(9604..10455)
                     /gene="yijO"
                     /locus_tag="ACHKIK_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO9083561.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10604..12337)
                     /gene="eptC"
                     /locus_tag="ACHKIK_002402"
     CDS             complement(10604..12337)
                     /gene="eptC"
                     /locus_tag="ACHKIK_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO9083562.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLHPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(12520..15171)
                     /gene="ppc"
                     /locus_tag="ACHKIK_002403"
     CDS             complement(12520..15171)
                     /gene="ppc"
                     /locus_tag="ACHKIK_002403"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO9083563.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(15523..16674)
                     /gene="argE"
                     /locus_tag="ACHKIK_002404"
     CDS             complement(15523..16674)
                     /gene="argE"
                     /locus_tag="ACHKIK_002404"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO9083564.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            16828..17832
                     /gene="argC"
                     /locus_tag="ACHKIK_002405"
     CDS             16828..17832
                     /gene="argC"
                     /locus_tag="ACHKIK_002405"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO9083565.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPCCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            17840..18616
                     /gene="argB"
                     /locus_tag="ACHKIK_002406"
     CDS             17840..18616
                     /gene="argB"
                     /locus_tag="ACHKIK_002406"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO9083566.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            18677..20050
                     /gene="argH"
                     /locus_tag="ACHKIK_002407"
     CDS             18677..20050
                     /gene="argH"
                     /locus_tag="ACHKIK_002407"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO9083567.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVGELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            20317..21234
                     /gene="oxyR"
                     /locus_tag="ACHKIK_002408"
     CDS             20317..21234
                     /gene="oxyR"
                     /locus_tag="ACHKIK_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO9083568.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(21217..22617)
                     /gene="sthA"
                     /locus_tag="ACHKIK_002409"
     CDS             complement(21217..22617)
                     /gene="sthA"
                     /locus_tag="ACHKIK_002409"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO9083569.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            22948..23598
                     /gene="fabR"
                     /locus_tag="ACHKIK_002410"
     CDS             22948..23598
                     /gene="fabR"
                     /locus_tag="ACHKIK_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO9083570.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFTSLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            23598..23957
                     /gene="yijD"
                     /locus_tag="ACHKIK_002411"
     CDS             23598..23957
                     /gene="yijD"
                     /locus_tag="ACHKIK_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO9083571.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(23997..25097)
                     /gene="trmA"
                     /locus_tag="ACHKIK_002412"
     CDS             complement(23997..25097)
                     /gene="trmA"
                     /locus_tag="ACHKIK_002412"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO9083572.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      25211..25401
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            25466..27310
                     /gene="btuB"
                     /locus_tag="ACHKIK_002413"
     CDS             25466..27310
                     /gene="btuB"
                     /locus_tag="ACHKIK_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO9083573.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            27255..28112
                     /gene="murI"
                     /locus_tag="ACHKIK_002414"
     CDS             27255..28112
                     /gene="murI"
                     /locus_tag="ACHKIK_002414"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO9083574.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            28484..>28572
                     /locus_tag="ACHKIK_002415"
     rRNA            28484..>28572
                     /locus_tag="ACHKIK_002415"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6556 a         7172 c         7865 g         6979 t
ORIGIN      
        1 actgataagc gtagcgcatc aggctatttt gcgttcaaac tcccggacaa gccgggagtt
       61 tggagtaggt tattcccact cttgcaggaa acgctgaccg tactggtcgg ctaccagcag
      121 agcggcgtaa acctgatctg gcgtcgcgcc gccaggcatg ttgtggatgg tttcaccttc
      181 tgcacatgcc gcttctgcca caattcgcat tttcgccggg acatcttctt taatatccag
      241 ttgagcgaga gttattggca aacctaccgc atggctaagt gcagctacgg tttcgatttc
      301 ctcaaccggc gcgttttcca gtaccagctg cgtcagcgta ccgaatgcca ctttttcacc
      361 gtgataatag tgatgcgcgt ccgggatagc ggtcaggccg ttatgcaccg catgagccgc
      421 cgccagtccg ccgctttcaa aaccaacgcc gcttaaatag gtgttcgctt caatcacgcg
      481 ttccagcgca ggggttacta catgctgttc ggcggcaagc atcgcttttt cgccttcttc
      541 cagcagagtg ttgtagcaca gttctgccag tgccagcgcc gcctgggtgc acttgccgcc
      601 cgccatagtg gtagcgccgc tacgggaaca ggcgcgtgct tcaaaccagg ttgccagcgc
      661 atcgccgata cccgccgcta acagacgtgc aggagcgccc gcgacgattt tggtgtcgac
      721 aatgaccata ttcgggttat ttggcaacag tagatagcgg tcaaactcac cctcatcggt
      781 gtagataaca gacaatgcgc tgcacggtgc atcggtagag gcgatagtcg gtgcgatcgc
      841 taccggaaca cccatgaaat gtgccagtgc tttggcagta tcgagggttt ttccgccacc
      901 gataccgaga attgcgccac actgcgcagt ctccgcgatg ccacgcagac gatcgatctc
      961 attttgcgaa cattcaccgc caaacggcgc aatttctact accagtccag catctttaaa
     1021 gcttttctcg acagtggatt gagcaaaacc taaaacaaat ttgtcaccca ccactaacca
     1081 gcgttctgcc agcggcttca ggtattcgcc cagacgatta atcacatcag cgccctggat
     1141 gtatttaccc ggtgattgaa taatgcggtc cataattgct cctttagaga tgagtagtgc
     1201 caaatgcggc attccagtcg tgttcgaact tctctatagc tgactctacc gcaggggtgt
     1261 tgagcatttg ttgcgctaca tctaaaggca gggtgatgga ctcacatcct gccagcaaac
     1321 aatccagtgc ctgacgcggc gttttaaagc tggctgcaag caccatgctt tctggcgcgt
     1381 gcatttctaa cagcgcttgc agctcctgaa ccgtacgaat gccgtccccg ccctgggcat
     1441 ctacgcggtt aacatacgga gcaacgtatt ttgcccctgc cagcgcggcc agtaaccctt
     1501 gtgcagcact gtacactgcg gtgccaagcg tagtaatacc ctcttttttc agcatcttaa
     1561 tggcggccag accttcggaa gtcaccggga ttttcaccac aatgcccgga atggcgtcgc
     1621 gcaggcgctt cgcttcttcc accataccct gcgcgtcacg actcatagtc tgagcaaaca
     1681 gaatgccctc atcactaatc gctttttgca gacgcggcag aacttcccat atggactcct
     1741 tgctggcagc gataatgctc gggttagttg tcaccccggc aatggggaat atgcgtgcca
     1801 gacgttcgac ttctgcgacg ttagcggtgt ccagatacag ttccatgatg tttcctttta
     1861 cagttccagt tcatgttgca gcaggctggc gatagcgtcg gcagaggcgg cgttaaccag
     1921 cgcgttacgg aattcttcgt gcatgatgcg gcgagcgagg cgcgagaaaa tgcgcatgtg
     1981 ctgatcgccc gcagcgtgtt tgttcagggt taacataatg atgaattgcg cttcatcatc
     2041 gccccagcgc accggcgctt gcagacgcgc tacactgatg gtggattgct caatgtgttc
     2101 tgatttgctg tggggaatgg caaaactaaa gcccagaccg gtagagaaaa cggcctcgcg
     2161 cgcccacaag tcggcttcca gtttacgcgg atagcggcag cggcccgcca gcagcaggtt
     2221 atcggtcatg cctttgagca cttcttcttt gctgcgtcag tcgctttcca gcgtgatgca
     2281 ctcggcggtg accagcggtg cgtcctgttg ggtcatgcgg aattgcgcca gcaggtgttc
     2341 cacttccagc gaagtacggc aggccattgc ctggttgagc aactggcggc attcgcggct
     2401 atcaagttgc gccattcgcg ctttcgccgc cggaattgac ggtgcgctca tgctcagctc
     2461 atccagccct aaaccgacca gcaacggcag cacggaacct ttcgctccca gctcaccgca
     2521 cagaccaatc catttgccct ggcgatgcac cgcttgcacg gcgtaatcga gcgcccgcaa
     2581 gaatgccgga ttcaggctgt tgtagtgacg agtaacctta gcattatcgc gatccaccgc
     2641 cagcagatat tgcgtcaggt cattactacc aatactaaag aaatcaatct cttcgcagca
     2701 ttgatcgatg atgaacatca ccgacggcac ttccagcata atgccgagct ggatcttctc
     2761 atcaaacgga atgtgttcgt tacgcagttg ctgtttggct tccgccagtt tttctttcac
     2821 ccataagatc tcttccattg aagagatcat cgggatcatg attttcaggc tgccgtgagc
     2881 ggaggcgcgc aggatcgacc gtagttgtgt ggtgaacagc gaggcgtatt cttcataaat
     2941 acgcacagcg cgataaccga ggaacgggtt agcttctgcc ggaatgttca gataatcaac
     3001 gggtttgtcg ccgccaatgt ccatagtgcg cacaataatg ctgcgtccgt tggcagactc
     3061 cagcgcctga caaaaaatgt tgtacagctc gctttcgccc ggtgcactgg tgcgatccat
     3121 atagagcatt tcagtgcgga acaaaccaac gccttccgca ccattgccga atgcggcctg
     3181 cgcttccacg gagtgagcga tgttagcggc aatttcaatg cggataccgt cagcggtacg
     3241 ggcttgttgg gtcagccaga cacgctgttg ctcacgcagg gcatcctgta cgcgggcttc
     3301 ttgctgataa taacgggcta cggcttcccc tggctcaacc acaatcgctc cggcgttgcc
     3361 gtcgatataa atcgtttgat gttgccacgg ggtaagggca tcaatatcca caccaaccag
     3421 cgtcggaatg ttgaacgaac gggcaaggat caccgtatgt gaggtggtgc cgccgctttt
     3481 gagcaacaat cctttgaggt gatttttatc cagttcgagg aactggctgg gggtcagttc
     3541 atcagccata caaatggcgg gctgcgtcag tttgcccggt gccgggaagc gttgctcacc
     3601 gtagatttgc tggagtaact ggaagcagac gtcgcgtacg tccagggcac gttcttgcag
     3661 gtagctgctg ctggaacggg aaaactcttc acaaaagtga ttcgcactgg cgacaatcgc
     3721 ttcggcgcag cttaatccgg cgctgacgcc tgccagtaaa tgttcgtgca gggaagtatc
     3781 gccagccagg gatcggtgcg cttccagaat cgcgctggtg gcaccgtcgc tatccagcag
     3841 acgaaactca atgtttttca gtaccagcgt caggccgttt tccagtgcgg attgctcggc
     3901 gtcaacgtct ttggctgcgg gaagattact cagcgcattg agatctaaag aagagatcgg
     3961 cgtcagaatg ccgcctgcgc taccgctgca cacggtgcgg gcgcggataa tttgcggatt
     4021 cagattggtc agtgaaaccg gcagtggttc cagttcgtca gatttaactt ccgccagcgg
     4081 tgcgtcgcag tgggggaatt catcgcgcag ccattggctt aaacgctggt gcgcttcctg
     4141 ttcgtcggcc ccggaaatca gtaaccggca gttatcgccc gccagcgtgt cggtgccaat
     4201 cagcgcaagg gcgcttttgg cgttgccctt gcggtcagtg cgcaggttat gccactcaat
     4261 ttgtgatgaa aaagtattac acagcgtttc aacgtggctt gccggacgtg catgtacgcc
     4321 gttaggtagc tcacaaataa attccacaat cagggccatt gcctctctcc cggtacggtt
     4381 ttctgtttac aggataagag cgcacggcaa cggcctgcca tgtgacaaat ctgccaaaag
     4441 ctggacaaat gtaatgtaac cgtcaatttg cgacgcgtct cacaagacgc cgttttgcgg
     4501 catgcttccg gattatcgca agttatgagg cggatcgcat ttttgtactg atattacaaa
     4561 agtccagtaa atggcctttt tatccactgt ttgctccgcc tgcgattgcc tattgtttcc
     4621 tccatcaaaa atatggacat gggctttcgc aagcccggga gcagcttatg aatgagttgg
     4681 tgcagatcct gaaaaatacc cgtcagcatt taatgacggg cgtttcacac atgattccct
     4741 tcgtggtatc gggcggtatt ttgctggcgg tttccgtcat gttgtatggc aaaggcgcag
     4801 tgccggatgc cgtagccgat ccgaatctga aaaaactgtt tgatatcgga gttgcaggtt
     4861 tgacgctgat ggtgcctttc ctcgccgctt acatcggtta ctccattgca gagcgttctg
     4921 cgctggcccc gtgcgctatc ggtgcctggg ttggtaacag ctttggtgcg ggcttctttg
     4981 gtgcgctgat cgccgggatt atcggcggca tcgtggtgca ttacctgaag aaaattccgg
     5041 tgcataaagt tctgcgctcg gtgatgccta tcttcattat tcctatcgtc ggcacactga
     5101 ttaccgcagg catcatgatg tggggcttgg gcgagcctgt aggggcgttg accaacagcc
     5161 tgactcagtg gcttcagggg atgcagcagg gcagcattgt tatgctggcg gtgatcatgg
     5221 gtctgatgct ggcgttcgat atgggcggtc cggttaacaa agtggcctat gccttcatgc
     5281 tgatttgcgt tgctcagggt gtttataccg tggtggccat tgctgccgtg ggtatttgta
     5341 tcccgccgct ggggatgggg ctggcgacgc tgattggtcg taaaaatttc tccgcagaag
     5401 agcgcgaaac cggcaaagcg gcgctggtga tggggtgcgt tggggttact gaaggggcga
     5461 ttcctttcgc cgctgccgat ccgctgcgtg tcattccttc catcatggtc ggttctgttt
     5521 gtggtgcggt aactgcggcg ctggtcggtg cgcagtgcta tgcaggctgg ggtggtctga
     5581 ttgtgctgcc ggtggttgaa ggcaagctgg gttatatcgc agcagtggct gtcggagcag
     5641 tggtgacggc tgtttgcgtg aacgtgctga aaagtctggc gcgtaaaaat gggtcttcga
     5701 ctgatgagaa ggaagacgac ctggatttgg attttgaaat taattaattg aggaacgagc
     5761 catgacgaaa attattgcag taaccgcgtg tccatctggt gttgcccata cctatatggc
     5821 ggcagaggcg ctggaaagcg ccgcgaaagc gaaaggctgg gaagtgaaag tagaaactca
     5881 ggggtcaatt ggtctggaaa acgaactgac tgcggaagac gtggcgagcg ccgatatggt
     5941 gattctgacc aaagatatcg gcatcaagtt tgaagagcgt tttgctggca agaccatcgt
     6001 gcgcgtcaac atcagcgatg cggtaaaacg tgctgacgcc attatgagca aaattgaagc
     6061 tcacctggcg caaaccgctt aagttcttct cccgctcgca agggcgggtt cgctttccca
     6121 caggagttcc tcatgacgaa tcgtatctct cgcctcaaaa ctgcactgtt ttccaatacc
     6181 cgtgaaatct cgctggagcg ggcgctgctt tataccgcca gccatcggca aaccgaaggc
     6241 gaaccggtga tattgcgccg ggcgaaagca acagcgtata tccttgaaca tgttgaaatt
     6301 tcgattcgtg atgaagaact gattgccggt aaccgcaccg taaaaccgcg cgccgggatt
     6361 atgtcgccgg aaatggaccc ttactggctg ctgaaagagc tggatcaatt cccgacgcgt
     6421 ccgcaggacc gctttgctat cagcgaagaa gataaacgta tctaccgcga agagttgttc
     6481 ccgtactggg aaaaacgttc gatgaaagat ttcatcaacg ggcagatgac agatgaagta
     6541 aaagccgcga ccagcacgca gattttcagc attaaccaga cggataaagg ccaggggcac
     6601 attattattg attacccacg cctgctgaat cacgggctgg gggagctggt ggcgcagatg
     6661 cagcaacatt gtcagcaaca gccggagaat cacttttatc aggccgcgct gttactgctg
     6721 gaagcctcgc agaaacacat tctgcgttac gctgaactgg cggaaacaat ggcggcaaac
     6781 tgcacagatg cccagcgtcg cgaagaactg ctgactattg cggagatctc ccgccataac
     6841 gccgaacata agccgcagac gttctggcag gcgtgccagt tattctggta catgaacatc
     6901 attctgcaat acgaatccaa cgccagttcg ctgtctttag ggcgcttcga ccagtatatg
     6961 ttgccgttct atcaggcttc tttaacccag ggcgaagatc cggcgttcct gaaagaactg
     7021 ctcgaatctt tatgggtgaa atgcaacgac atcgtgctgt tgcgctccac cagcagcgcg
     7081 cgttatttcg caggtttccc gaccggctat accgcactgc tcggcgggtt aaccgagaac
     7141 ggacgtagcg cggtgaacgt gctttcgttc ctttgccttg acgcctatca aagcgtgcaa
     7201 ttaccgcaac cgaacctcgg cgtgcgcact aacgccttga tcgacacgcc gttcctgatg
     7261 aaaaccgccg aaaccattcg cctcggcacc ggtattccgc aaatctttaa cgatgaagtg
     7321 gtggtaccag cgttcctcaa ccgtggcgtt tcgctggaag atgcgcgcga ctattccgta
     7381 gtgggctgtg tggaattatc tattcccggc agaacctacg gcttgcatga catcgcgatg
     7441 tttaatctgc tgaaagtgat ggaaatctgc ctgcatgaaa atgaaggcaa tgctgcgctg
     7501 acttatgaag gtttactgga gcagatccgc gccaagatca gccactacat caccctgatg
     7561 gttgagggca gcaatatttg tgatatcggc catcgcgact gggcacctgt accgctgctc
     7621 tcgtctttta tcagcgattg tctggaaaaa ggtcgcgata ttaccgatgg cggcgcgcgt
     7681 tataacttct ccggcgtaca ggggatcggt atcgccaacc tgagcgattc tctccatgcg
     7741 ttgaaaggga tggtttttga gcaacagcgt ttaagttttg acgaattgct gtcggtatta
     7801 aaagccaact ttgcaacgcc agaaggcgaa aaagtccgtg ctcgcttaat taaccgcttc
     7861 gagaaatacg gtaacgatat cgacgaggtg gataacatca gcgccgaact gttacgccac
     7921 tactgcaaag aagtggaaaa ataccagaac ccgcgcggtg gttacttcac gccgggatcg
     7981 tataccgttt ctgctcacgt cccgttggga tcggtggttg gcgcgacgcc agacggtcgt
     8041 tttgccgggg aacaactggc agacggcggc ttgtcaccta tgctgggcca ggacgcacaa
     8101 gggccaacag cggtactgaa gtcagtcagt aagctcgata atacgctgct gtctaacggt
     8161 acgttgctga acgtgaaatt cactccggcg accctggaag gtgaagcggg attacgcaaa
     8221 ctggccgact tcttacgggc gtttacccag cttaagttgc agcatattca gtttaacgtg
     8281 gtgaacgccg acacgttgcg ggaagcgcaa cagcgcccac aagattatgc cgggctggtg
     8341 gtgcgcgttg ccggatacag cgccttcttt gtcgaactgt cgaaggagat ccaggatgac
     8401 atcatccgcc ggacagcgca tcagctgtaa cgttgtggaa acgcgccgca atgatgtggc
     8461 gcgcattttc aacattcagc gttattcact gaatgacggc gagggcattc gtacggtggt
     8521 cttttttaaa ggctgtccgc atctttgccc atggtgtgct aatccggagt cgatctccgg
     8581 caaaatccag acggtacgca gagaggcgaa atgtctgcac tgtgcgaaat gtttgcgtga
     8641 tgcggatgaa tgcccctccg gggcgtttga acggattggt cgcgatatca gccttgacgc
     8701 gctggaacgg gaagtgatga aagatgacat tttctttcgc acgtccggcg gcggcgtcac
     8761 gctttctggc ggcgaagtgt taatgcaggc ggagtttgct acccgttttt tacagcggct
     8821 gcggttgtgg ggcgtgtcat gcgccattga aactgccgga gacgtgccag ccagcaagct
     8881 gttaccactg gcgaaattgt gcgatgaagt gctgttcgat ttaaaaatta tggacgcgac
     8941 tcaggcgcgg gatgtggtga agatgaacct gccacgcgtg ctggagaatc tgcgtttgct
     9001 ggtgagtgag ggtgtcaacg tgatcccgcg tttaccgttg atccctggtt tcacgctcag
     9061 ccgggagaat atgcagcagg cgctggatgt actgatcccg ctgaaaatca ggcagatcca
     9121 tctgttaccg tttcatcagt acggcgaacc gaaataccgc ctgctgggga aaacatggtc
     9181 gatgaaagag gtgcctgcgc cgtcgtcagc cgatgtgaca acgatgcgcg aaatggcaga
     9241 acgggccgga tttcaggtta ccgtgggagg ttaaaatggc atacctggtg gcagtaaccg
     9301 cctgcgtaag cggcgtggcg catacttata tggcggcgga acggctggaa aagttgtgcc
     9361 agttagagaa gtggggcgtt agcattgaaa ctcagggcgc gctgggaacg gagaatcgtt
     9421 tagcggacga ggatatccgt cgggcggatg ttgctctgtt gattacggat atcgagcttg
     9481 ccggtgccga gcgatttgaa cattgccgct atgtgcaatg cagcatctac gcattcctgc
     9541 gtgagccgca gcgggtaatg agcgcggtgc gcaaagtgct ttctgcgccg cagcaaaccc
     9601 atcttattct ggagtagtcg gtttttctgt cagttggctg tggtactgcc gacgatattc
     9661 cgacggcgag cgttcggtgt ttttacgaaa cagacggcag aagtagttgc tgtcgacaaa
     9721 gccgcaggcg tgcgccactt cttttacctt caggtcatag cctttcaata acgtcttagc
     9781 gtgctccagt cgcgtgtgat tcaggtattc gttaaaacca atggcccccg ttttttgaaa
     9841 caggtgcgag agatagtttg gtgaaatata gaatgcctgt gcgacagatt cgcgggtaag
     9901 cgcggaggca tagcgttcgt cgatataatc gcgaatagct tcaaatagtg cctggctgcg
     9961 tgaggcggtc tggatttggc tgccgagcaa atcgcggcag tggctaagca ggctggcgac
    10021 gataagcctt gccgtttgct gctcctgcgg ctgcatttgc atttcattga gcgtttgtag
    10081 cagaaaagaa cctatgcgtg ggccgcgccg ggcgacgtgt tgcttcgcca gattttgata
    10141 ttgtttgcca tcccattgca cgacgctgaa gccgagttgc tgtttgccaa acagcacgct
    10201 aaaggtagta gcgggggctt gccattgtgg aaaattccag ccaccagcag gaacgtacag
    10261 cacatcgccg ggaactaacg ttgctccagc gccggtatca acaaactcac cttccagcac
    10321 aatttccagc cgtggaaaat cgacctgata cgcgagatcg ggcaccgggc cgttactgct
    10381 ggcaaagtaa atctggcgca gggacagcgg gccgttgatc aggcgggaga gcagatagct
    10441 gacgtcgtga tacatgttat tcccttaggt atgtagacgg gaaaacagta actggcatta
    10501 ttaaaaaagg ccacctgaaa agtgtggcct gaggggagtt cgatgtaaac attgcggcag
    10561 tatcgcatcc ggcaaatagc gccagacgcg atacaaaggt catttactga ttacccacct
    10621 gatcgccata cggcagcgtg tcgtaatcga tcagcgcgtt tttcttgtac gggttaccaa
    10681 tccagcgggt agtttctttg aactgcggat tcaccactga acgggttgga tcgtaaccgt
    10741 cgtaagataa gcctgctaaa tctgaccagg tgtggatcag ttccgccagg ctgtatttac
    10801 gatcaacatc ctgcgagaaa tcacgcggat gagtcgcttg ccatttttcc gacgtccaca
    10861 gcaggaacgg aatggtgtac atgtggcgcg tcgggttgtc ttcgttacgc ccctgagttt
    10921 tgtgcggcgg cgtgtcgtaa acctcttcac cgtggtcaga gaaataaacc aggaaaccgt
    10981 tcgggtttgc tgctttaaag tctttaatca ggctggcaac cacatgatcg ttatacaggt
    11041 tagcgttgtc ataatcgtta tatgactcca gctcttccgc gtttaatcca ggcggaacat
    11101 gatcggtatt gccatcaaac ttgccctgat tttccgggta gcggtatttg tatttgatat
    11161 gcgtacccag cagatgaacg atgatcagtt tcttcggcgc cgggtccttc agcacttcct
    11221 ggaatggttt cagcacgttg gtgtcgtatt cacgcgcact ctgcgtacgt tgctggttca
    11281 tatagaactg tttgtcggtc tgcttcgaaa ataccgtcag catggtattg cgggcggtca
    11341 tcgtctgctg gttggtgatc cagaaggttt tataacctgc ctgtttcatc atgttcatca
    11401 gcgacggttg tgtcaggtac aggtccgggt tcttttcatt agcaaaggtc agcgcctgtt
    11461 gcaggatttc aatggtgtac ggacgagacg taaccacgtt attaaacacg gtcagattcg
    11521 gatcggtttt atgcagcgca tccagctccg gcgtggtttc acgcggataa ccgtacagac
    11581 tcatgcgccc gcgttgggtc gattcgccaa tcaccagcac taaggtacgc ggttcgttac
    11641 ccgattcatc tttgaaatta gccagaggcg gcaaggcatt attttcattc agtaactttg
    11701 ttagcgagtt tagttgctga cgatactgat aatagccggt caggaattgc cacggtgcgg
    11761 caggctccat acgcgaggcc aggttatcca acgttttctc aaacggcttg tttttgataa
    11821 acgtattcat ggcgatcgga tgcagaatca agccataaag cagggcaaaa gagacaacat
    11881 aacgccacgg ctttggaata tagaccgggt gcaggcgtgt ccacagcaga actgccaccg
    11941 ccgtataggc cagcgcgata agcacaattt tcaggctgaa atactggctt aaatactcgc
    12001 tggcttcgtt ggtgttggtt tcgaacatca caaacagaac gctctgcgag aactcctgcc
    12061 cgtagatgac gtagtagcat agcgccgcca gagaggccgc ccatagcacc acgccgatta
    12121 ctgcggcaat aattttaatc cgcttcggaa agaggaatac cgggatcaac cacagcgagc
    12181 tgaataacag cgagtcgcga atgccgttgg tgccactata accactgatg taaataatgg
    12241 cctgtagcag agtagagaaa aaccaaaagt agagcagtgc ccaacccagg gctttccagc
    12301 taaaaagggg tttagcctgg acttctgtgg aatgcatagt aagaacctgt cttgaaaaaa
    12361 atatcgccga atgtaacgac aattccttaa ggatatctga aggtatattc agaatttgaa
    12421 taaaatgcag acagaaatat attgaaaacg agggtgttag aacagaagta tttcagaaaa
    12481 ccctcgcgca aaagcacgag ggtttgcaga agaggaagat tagccggtat tacgcatacc
    12541 tgccgcaatc ccggcaatag tgaccattaa cgcttgttcg acgcgaggat ccggttcctg
    12601 gccttctttt tctgcctggc gggagcggtg cagtaactcg gcctgcaata cgttcagcgg
    12661 gtcggtgtaa atattccgta gctgaataga ctctgcaatc cacggcagat cggccattag
    12721 atgggaatcg ttggcaatcg ccagcaccac tttgatgtct tcttcttgca ggttgcgtaa
    12781 ctctttacct aacggccaca gtgctttgtc taccaggcgt tggtcatagt attccgccag
    12841 ccacaggtct gctttggcga agaccatctc cagcatgccg agacgcgtcg agaagaatgg
    12901 ccaatcgcgg cacatggctt ccagctcgct ctgtttgcca tcttcgacca ctttttgcag
    12961 cgccgtacct gcacccagcc aggcggggag catcaggcgg ttttgcgtcc aggcaaaaat
    13021 ccacggtatg gcgcgcagcg actcgacgcc gccggttggg cgacgtttcg ccggacgtga
    13081 acccaacggc agtttgccca attcttgttc cggcgtagcg gagcggaagt aaggcacaaa
    13141 atctttgttt tcacgtacgt agccacggta gagatcgcag gagatgactg acagttcatc
    13201 cataatgcga cgccagctct ctttcggctc aggtggtggc agcaggttgg cttccagaat
    13261 agccccggtg tacagcgaca ggctgctgac ggtgatttct ggcagaccat atttaaagcg
    13321 gatcatctcg ccctgttcag tcacgcgcag gccacctttc aggcttcctg gcggttgtga
    13381 cagcagcgcc gcatgagcag gtgcgccgcc gcgaccaatg gaaccgccgc gaccgtggaa
    13441 caacgtcagc tcaatacccg ctttttcgca ggttttgatt aatgcatcct gtgcctgata
    13501 ttgcgcccag gaagctgcca tcactcccgc atcttttgct gagtcggaat agccaatcat
    13561 caccatctgt ttgccctgaa tcaggccacg ataccagtca atattgagca gctgggtcat
    13621 gacatcgttg gcgttgttca gatcatcgag ggtttcaaac agcggagcaa ccggcatcgc
    13681 aaacccgata cccgcttctt tcagcagcag gtggacagcc agtacgtcgg acggcgtttt
    13741 cgccatcgag atcacgtagg cggcaatgga gccttgcggt gcttcggcaa tcacctggca
    13801 ggtatcgagc acttcgcgcg tttcggcgct tggttgccag ttgcgcggca gaagcggacg
    13861 tttggagttc agttcgcgga tcaggaacgc ctgtttgtcg gcctctgacc agctttcgta
    13921 gtcgccgata ccgaggtagc gggtcagctc gcccagcgct tcggtatgac gcgtgctctc
    13981 ctgacggata tcaatacgga ccagcggtac gccgaaacat ttcacgcggc gcagggtgtc
    14041 gagcagatcg ccgttggcga taatacccat gccacacgcc tgaagtgact ggtagcaagc
    14101 gtagagcggt tcccacagtt cttcgttttg tgtcagcagg ccttctggtt ttggcagttc
    14161 ttcgcctttc aggcgcgctt ccagccatgc ctgtgtcgcc atcaggcgag aacgcaggtt
    14221 tttcatcaga tagcgatacg gttctgcggc accttcttcg ccaaccagcg ccagcagttc
    14281 aggggtcgct tcaaccatcg acagttcaga aaccagcacc tgaatatctt tcaggaacaa
    14341 atcggtggct ttccagcggc tgagtagcag gacgtggcgg gtgatatcgg cagtgacgtt
    14401 cgggttgccg tcgcggtcgc cgcccatcca cgaagtaaaa cggaccggaa caaattcgac
    14461 gggcagtttg tagccgaggt tctcttccag ttgttcgttc agttcgcgca ggtaatttgg
    14521 tacgccttgc cacaggctgt tttccactac ggcaaagccc catttggctt catctaccgg
    14581 gcttggacgc agcttacgga tttcatcggt atgccatgac tgggcgatta actggcgcag
    14641 gcggcgcatc agctggttgt gttcgtagtc agcgatatct ttgttatcga gctgttttaa
    14701 acaggcgttc acttccacca ttttgtggat cagtgtacga cgggtaattt cggttgggtg
    14761 agccgtgagg accagttcca gcgacagcga ttccactgct tttttgatgg tgtcttcgct
    14821 cagttccggc tggtttttca gtttacgcag ggtgcgggcg atcacttccg ggttgctggc
    14881 agcttcgcct ttcggcgaaa tgctgtggta ttgctcggcg gtgttggcca ggttcaggaa
    14941 ctgactaaac gcacgcgcaa cgggcagcag ctcgtcgttc gacaaatttt gtaaggtggt
    15001 gagcaactcc tggcggttag catcattgcc agcgcgtgaa gatttcgaca acttacggat
    15061 agtttctacg cgttcaagaa tgtgttctcc caacgcatcc ttgatggttt ctcccagcac
    15121 tttgccgagc atactgacat tactacgcaa tgcggaatat tgttcgttca tattacccca
    15181 gacaccccat cttatcgttt gatagccctg tatccttcac gtcgcattgg cgcgaatatg
    15241 ctcgggcttt gcttttcgtc gtcttttata aagccacgta aaagcggtga cgtcaaatgc
    15301 tgcgaaatcg cttcagcaaa cgaataaata gcaggaattt acgccattaa attcacgacg
    15361 ctttaaataa gcgtaactta tggaaatgtt aaaaaatcgc catacataac accaaaggtg
    15421 taggtcggat aagatgcgca agcatcgcat ccgacattat tgcggcacag gagtttggca
    15481 acggtgccgg atgcggcgcg agcgccttat ccggcctacg ttttaatgcc agcaaaaatg
    15541 gtgaattacc tgggttatca gttcgcgggt gggcttgata aaccgtgttt ccagatattc
    15601 atcaggttga tgagcctgat taattgagcc aggccccaac accagcgtcg ggcataacgt
    15661 ttgaataaac ggcgcttcgg tacagtagtt caccacttcg gtctttgctc cgagcaattt
    15721 ctcaactact tcaaccagtt gatgattcgg tgggcattca tagccaggga tcggcggatg
    15781 cagctcgtcg accgtcagac gacccggcca gcgttcgctc accggagcca atgcatcgtt
    15841 gagcaaacca ttaagttcat tgagtgtcat gccaggcagc ggacgaatat ccatatgcaa
    15901 ctcacagcaa gcgcaaatac ggttagaagc gtcgccaccg tgaatatgcc cgaggttgag
    15961 cgtagggtat ggcacggtaa acgcttcgta gtgataacgt tctttcaggt tatcgcgcaa
    16021 ttgcaaaata tgcccgatgg cgtcgtgcat tagttcgata gcgttaactc cgcgtgctgg
    16081 atcgctggag tgccccgact ggccctgaat acggatggcg ttagagatat gacctttatg
    16141 tgcgcgtacc ggttgtagtg acgtcggttc gccaatgatg gcgcaatccg ggcgcagggc
    16201 ggtagtttcg gcaaaataac gcgctccggc catactggtt tcttcatcag cagtcgccag
    16261 aatgtagagc ggttttttca gtttcgtgac gtcgacatcg cgtagcgcat caaggataaa
    16321 cgcaaaaaag cctttcatgt cggcggtgcc taagccgtaa agcttgccgt catgctccgt
    16381 cagtgtaaac ggatcgcgcg tccagcgacc gtcatcaaat ggcaccgtat cggtatgccc
    16441 cgccagcaac aagccgccag ccccctgtcc gatacttgcc agcatattga atttgttgcg
    16501 agttcctggc acaggctgca cttccacatt gaagcctaaa tctttaaacc agtccgccag
    16561 cagagtgatt aaatctgcat tgctttgatc gagtgcctct tccgtggcgc ttattgaagg
    16621 tgtggcaatc agagcacggt aaatctcgat aaatggcggt aatttgtttt tcattgttga
    16681 cacacctctg gtcatgatag tatcaatatt catgcagtat ttatgaataa aaatacacta
    16741 acgttgagcg taataaaacc caccagccgt aaggtgaatg ttttacgttt aacctggcaa
    16801 ccagacataa gaaggtgaat agccccgatg ttgaatacgc tgattgtggg tgccagcggc
    16861 tacgctggcg cagagctagt gacctatgta aatcgccatc cgcatatgaa cataaccgct
    16921 ttgactgttt cagcgcaaag caatgatgcg ggaaagttaa tctccgattt gcatccgcag
    16981 ctaaaaggca tcgttgatct gccgttgcag ccgatgtcgg atatcagcga gtttagccca
    17041 ggggtggacg tagtgtttct cgccaccgcc catgaagtta gccacgattt agcgccgcag
    17101 tttcttgaag cgggctgcgt ggtgttcgac ctttccggcg cgtttcgtgt taacgacgcc
    17161 accttctatg aaaaatatta cggctttacc catcaatacc cggaactgtt ggaacaggca
    17221 gcctacggtc tggcggagtg gtgcggtaat aaattaaaag aagcgaattt gattgcggtg
    17281 ccgggctgtt atccgacggc ggcacagctg gcgctgaaac cgttgattga tgccgatctt
    17341 cttgacctca atcagtggcc ggtgatcaac gccaccagcg gcgtgagcgg tgcaggacgt
    17401 aaggcagcga tttcaaatag tttttgtgaa gttagcctgc aaccgtatgg cgtctttacc
    17461 catcgccatc aaccagagat cgccacacac ctcggtgctg acgttatctt caccccgcat
    17521 ctgggcaatt tcccgcgcgg cattcttgaa accattacct gccgcctgaa accgggtgtg
    17581 acccaggcgc aagtggcgca agcgttacag caggcgtatg cccataaacc gctggtgcgg
    17641 ctgtatgaca aaggcgttcc ggcgctgaaa aatgtcgttg ggctgccatg ttgcgatatc
    17701 gggtttgccg ttcagggcga gcatctgatt attgtggcga ccgaagacaa cttactgaaa
    17761 ggcgcggcgg cacaagcggt acagtgcgcc aatattcgtt tcggctatgc ggaaacgcag
    17821 tctcttattt aagggtgcaa tgatgaatcc attaattatc aaactgggcg gcgtactgct
    17881 ggatagtgaa gaggcgctgg aacgtctgtt tagcgcactg gtgaattatc gtgagtcaca
    17941 tcagcgtccg ctggtgattg tgcacggcgg cggttgcgtg gtggatgagc tgatgaaagg
    18001 gctgaatctg ccggtgaaaa agaaaaatgg cctgcgggtg acgcctgcgg atcagataga
    18061 cattatcacc ggagcactgg caggaacggc aaataaaacc ctgctggcat gggcgaagaa
    18121 acatcagatt gcggccgtag gtttgtttct cggtgacggc gacagcgtca aagtgaccca
    18181 gcttgatgaa gagttaggtc atgttggact ggcgcagcca ggttcgccta agcttatcaa
    18241 caccttgctg gagaacggct atctgccggt ggtcagctcc attggcgtaa cagacgaagg
    18301 gcaactgatg aacgtcaatg ccgaccaggc ggcgacggcg ctggcggcaa cgctgggcgc
    18361 ggatctgatt ttgctctctg atgtcagcgg cattctcgac ggcaaagggc aacgcattgc
    18421 cgaaatgacc gccgcgaaag cagaacaact gattgagcag ggcattatta ctgacggcat
    18481 gatagtgaaa gtgaacgcgg cgctggatgc tgcgcgcacg ttgggccgtc cggtagatat
    18541 cgcctcctgg cgtcatgcgg aacagcttcc ggcactgttt aacggtatgc cgatgggtac
    18601 gcggatttta gcttaagttt tgttggccgg aggcgcagct ttccggcatt gaatttcaaa
    18661 ataaggaaac agagttatgg cactttgggg cgggcgtttt acccaggcag cagatcaacg
    18721 gttcaaacaa ttcaacgact cactgcgctt tgattaccgt ctggcggagc aggatattgt
    18781 tggctctgtg gcctggtcca aagccctggt aacggtcggc gtgttaaccg cagaagagca
    18841 ggcgcaactg gaagaggcgc tgaacgtgtt gctggaagat gttcgcgcca ggccacaaca
    18901 aatccttgaa agcgacgccg aagatatcca tagctgggtg gaaggcaaac tgatcgacaa
    18961 agtgggccag ttaggcaaaa agctgcatac cgggcgtagc cgtaatgatc aggtagcgac
    19021 agacctgaaa ctgtggtgca aagataccgt tggcgagttg ctgacggcta accggcagct
    19081 gcaatcggcg ctggtggaaa ccgcacaaaa caatcaggac gcggtgatgc caggttacac
    19141 tcacctgcaa cgcgcccagc cggtgacgtt cgcgcactgg tgcctggcct atgttgagat
    19201 gctggcgcgt gatgaaagcc gtttgcagga tgcgcttaag cgtctggatg tcagcccatt
    19261 aggctgtggc gcgctggcgg gaacggccta tgaaatcgac cgtgaacagt tagcaggctg
    19321 gctgggcttt gcttcggcga cccgtaacag tctcgacagc gtttctgacc gtgaccatgt
    19381 gttggaactg ctttctgctg ccgctatcgg catggtgcat ctgtcgcgtt ttgctgaaga
    19441 tctgattttc tttaacaccg gcgaagcggg gtttgtggag ctttctgacc gcgtgacttc
    19501 cggttcatca ttaatgccgc agaagaaaaa cccggatgcg ctggagctga ttcgcggtaa
    19561 atgcggtcgg gtgcaggggg cgttaaccgg catgatgatg acgctgaaag gtttgccgct
    19621 ggcttacaac aaagatatgc aggaagacaa agaaggtctg ttcgacgcgc tcgatacctg
    19681 gctggactgc ctgcatatgg cggcgctggt gctggacggc attcaggtga aacgtccacg
    19741 ttgccaggaa gcggctcagc agggttacgc caacgccacc gaactggcgg attatctggt
    19801 ggcgaaaggc gtaccgttcc gcgaggcgca ccatattgtt ggtgaagcgg tggtggaagc
    19861 cattcgtcag ggcaaaccgc tggaagatct gccgctcgac gagttgcaga aattcagccc
    19921 ggtgattgac gaagatgtct atccgattct gtcgctgcaa tcgtgcctcg acaagcgtgc
    19981 ggcaaaaggc ggcgtctcac cgcagcaggt ggcgcaggca attgcttttg cgcaggcgcg
    20041 gttggggtaa gaacatttat atgtataaat ttgagcctgg cttatcgccg ggctttttta
    20101 tggcaaaaaa aagcggatcc tggagatccg caaaagttca cgttggcttt agttattcga
    20161 gttgagaaac tctcgaaacg ggcagtgact tcaagggtta aaagaggtgc cgctccgttt
    20221 ctgtgagcaa ttatcagtca gaatgcttga tagggataat cgttcattgc tattctacct
    20281 atcgccatga actatcgtgg caatggagga tggataatga atattcgtga tcttgagtac
    20341 ctggtggcat tggccgaaca ccgccatttt cggcgtgcgg cagattcctg ccacgttagc
    20401 cagccgacgc ttagcgggca aattcgtaag ctggaagatg agttgggcgt gatgttgctg
    20461 gagcggacca gccgtaaagt gttgttcacc caggcgggaa tgctgctggt agatcaggcg
    20521 cgtaccgtac tgcgtgaggt gaaagtcctt aaagagatgg caagccagca gggcgagacg
    20581 atgtccgggc cgctgcacat tggtttgatt cccacagttg gaccgtacct gctaccgcat
    20641 attatcccta tgctgcacca gacctttcca aagctggaaa tgtatctgca tgaagcacag
    20701 acccaccagt tactggcgca actggacagc ggcaaactcg attgcgtgat cctcgcgctg
    20761 gtgaaagaga gcgaagcatt cattgaagtg ccgttgtttg atgagccaat gttgctggct
    20821 atctatgaag atcacccgtg ggcgaaccgc gaatgcgtac cgatggccga tctggcaggg
    20881 gaaaaactgc tgatgctgga agatggtcac tgtttgcgcg atcaggcaat gggtttctgt
    20941 tttgaagccg gggcggatga agatacacac ttccgcgcga ccagcctgga gacgctgcgc
    21001 aacatggtgg cggcaggtag cgggatcact ttactgcctg cgctggctgt gccgccggag
    21061 cgcaaacgcg atggggttgt ttatctgccg tgcattaagc cggaaccacg ccgcactatt
    21121 ggcctggttt atcgtcctgg ctcaccgctg cgcagccgct atgagcagct ggcagaggcc
    21181 atccgcgcac gaatggatgg ccatttcgat aaagttttaa aacaggcggt ttaaaccgtt
    21241 taacgcagct acccgatagg cttccgccat cgtcgggtag ttaaaggtgg tgttgacgaa
    21301 gtactcaata gtgttgccgc cacctttctg ttccataatc gcctgaccga tatgaataat
    21361 ttcggcagcg cgctcgccaa agcagtgaat acccagaatc tcttttgttt cccgatggaa
    21421 caaaattttc agcgtgccca cgttcatgcc gacgatttgt gcgcgtgcca gatgtttaaa
    21481 ctgggcgcgg cccacttcat atggcacttt cattgcggtc agctgctgtt cggttttgcc
    21541 cacagagctg atttccggga tggtgtaaat gccggtaggg atatcttcaa tcagatgtgc
    21601 ggtggcttcg ccttttacca gcgcctgcgc ggcaatgcgc ccctggtcat aggccgctga
    21661 tgccaggctc ggataaccaa tcacgtcgcc caccgcgtaa acgtgcggct gtgcggtctg
    21721 atacatgctg ttgaccttca gctgtccgcg gctgtcagtt tccagcccaa tgttctgtaa
    21781 cgccagcgaa tcggtattac cggtgcgacc gttggcatag agcaggcagt cagctttcag
    21841 ttttttaccc gacttcaaat gcatgatcac accatcgtca cagccttcga tcttctcgta
    21901 ctcttcgttg tgacgaatca ctacgccact gttccagaag tgataggaga gagaatctga
    21961 catctcttga tcgagaaatg ccagcaggcg atcgcgggtg ttgatcagat ccacttttac
    22021 atccataccg cggaagatcg acgcatattc acagccgatc actccagcac catagataag
    22081 tacatggcgc ggttcgtggt gcatgctaag aattgagtcg ctgtcgtaaa tgcgtggatg
    22141 ggtgaaatca acatctgttg gatgatatgg acgagagccg caggcaataa caaatttttc
    22201 agcggttagt gtttcaacgc tgccgtccgg gcaatccagc gccaacgtat gctcgtcaac
    22261 aaagcgagcg tttccctgca atatttcaca gtgattacgt tcgtaaaatc cctgacgcat
    22321 gcgcgtttgt tgattaatca cgttatcggc atggttaagg atatcggcaa aagaagagcg
    22381 gagcagtcgg gaatggtcgc tgtaaagtgg gttttgattg aattctataa tgcggctgac
    22441 ggcgtgacgg agagctttcg acgggatggt gccccagtgg gtgcaaccgc cgccaacatt
    22501 ttgataacgc tcgataactg cgacgcgcgc accttgctta accaggccca ttgcagcgcc
    22561 ttcgccgccg gggccggaac ctattactat ggcatcgtaa tcgtaggaat gtggcatggt
    22621 agggcttacc tgttcttata cataaaagca acagaatggt aacattttat cgcgggtaag
    22681 ccaattgatc cccgtcattt atctggctat atcctgagcg gcctttgctt tgtctgtttc
    22741 ttacttttgc cctgacgttt tattggattt ttatcgacga tactctccgt ttaagcggca
    22801 ggtttccgct gtacgtaaaa gaaccggcca aagaattgca gtaaatatgt tttattgcgt
    22861 taccgttcat tcacaatact ggagcaatcc agtatgttca ttctctggta tagtgccagc
    22921 agtacttttg gcaaggattc agacatcgtg atgggcgtaa gagcgcaaca aaaagaaaaa
    22981 acccgccgtt cgctggtgga agccgcattt agccaattaa gtgctgaacg cagcttcacc
    23041 agcctgagtt tgcgtgaagt ggcgcgtgaa gcgggcattg ctcccacctc tttttatcgg
    23101 catttccgcg acgtagacga actgggtctg accatggttg atgagagcgg tttaatgcta
    23161 cgccaactca tgcgccaggc gcgtcagcgt atcgccaaag gcgggagtgt gatccgcacc
    23221 tcggtctcca catttatgga gttcatcggt aataatccta acgccttccg gttattattg
    23281 cgggaacgct ccggcacctc cgctgcgttt cgtgccgccg ttgcgcgtga aattcagcac
    23341 ttcattgcgg aacttgcgga ctatctggaa ctcgaaaacc atatgccgcg tgcgtttact
    23401 gaagcgcaag ccgaagcaat ggtgacaatt gtcttcagtg cgggtgccga ggcgttggac
    23461 gtcggcgtcg aacaacgtcg gcaattagaa gagcgactgg tactgcaact gcgaatgatt
    23521 tcgaaagggg cttattactg gtatcgccgt gaacaagaga aaaccgcaat tattccggga
    23581 aatgtgaagg acgagtaatg aaacaagcaa atcaagatag aggtacgctg ctgctggcgt
    23641 tagttgctgg cttatcgatt aatggtactt tcgcagcgct gtttagctcc attgtgccat
    23701 tttctgtatt cccgattatt tccctggtgc tgacggttta ctgcctgcat caacgttatc
    23761 ttaatcgcac tatgccggta ggcttgccgg gtctggcagc tgcctgtttt attctcggcg
    23821 tactgctgta cagcacggta gttcgtgcgg aatatccgga tatcggctct aacttcttcc
    23881 cggcagtact ctccgtcatt atggtgttct ggattggcgc gaagatgcgt aaccgtaagc
    23941 aggaagttgc tgaataatcg gtattatgcc ggatgctggc gcatccagca tgggttttac
    24001 ttcgcggtca gtaatacgcc gcactccata tggtgcgtgt aggggaactg atcaaacaga
    24061 gccagacgtt cgaccttgtg cgtctggctt aatgtttcca gattcttgca taacgtttcc
    24121 gggttacagg agatgtacaa aatacacgga tacgcctgca ccattttctc ggtttcactg
    24181 tccagaccgc tgcgcggtgg gtcgacaaaa atggtttcgc actgataact ctttaagtcg
    24241 atcccttgca ggcggttaaa ctcgcgcaca ccattcatcg cctgagtaaa ttcttctgcc
    24301 gccatacgaa taatttgtac gttatcaata tggttagctg cgatgttgta ttgcgcagca
    24361 gcaaccgacg gcttagcgat ttcggtggct aatacccgat caaaattgcg cgccagcgct
    24421 aatgaaaagt taccgttgcc gcagtacagc tccagtaaat cgccttttga gcctttggtt
    24481 acgtccagcg cccattccag catctgaata ttcatcgccg cgttcggctg ggtaaagctg
    24541 ttttctacct gacggtagat catctctttc cctgcgaccg gcagacgctc gtcgatgtaa
    24601 tcttgatcca gctcgatttt ggttttcgtt gcccgaccaa tcagatgcac attcagattc
    24661 tgcgcgcgca gtgcatcgcg cagggcctcc gcttcctgac gccactcatc atccagcttc
    24721 ttatggtata gcagggaaac caccgcctga ttactcagcg tggtgaggta atcaatctgg
    24781 aacaacttgt ggcgcagaac gggattatta cgcacacccg caatcatcgc cgtcatcaac
    24841 tggttgataa gttcactggc ggcggggaag ctatccacgc ggatgcggct tttggtttgt
    24901 tgatcgaaaa tgatgtgata caggtcatcg ccatcgtgcc agatgcggaa ctccgcgcgc
    24961 atccggtaat gactgaccgg cgagcgaaac acttccggaa ccaggtcaga aaacggtgcc
    25021 atcatacttt gcaaacgtac cactttttcg gctaactgcg cttcatactg ttctgttgga
    25081 aggtgttcgg gggtcatgat gtatcctgaa aaattaaagt acgcggcgat tgtagggatt
    25141 gctcatcaga tgtccagatc ttgatgaatt cctatttgtg agctacgtct ggacagtaac
    25201 ttgttacaac ctgtagcatc cacttgccgg tcctgtgagt taatagggaa tccagtgcga
    25261 atctggagct gacgcgcagc ggtaaggaaa ggtgcgatga ttgcgttatg cggacactgc
    25321 catccggtgg gaagtcatca tctcttagta tcttagatac ccctccaagc ccgaagacct
    25381 gccggccaac gtcgcatctg gttctcatca tcgcgtaata ttgatgaaac ctgcggcatc
    25441 cttcttctat tgtggatgct ttacaatgat taaaaaagct tcgctgctga cggcgtgttc
    25501 tgtcacagcc ttttccgctt gggcacagga taccagcccg gatactctcg tcgttactgc
    25561 taaccgtttt gaacagccgc gcagcactgt gcttgcacca accaccgttg tgacccgtca
    25621 ggatatcgac cgctggcagt cgacctcggt caatgatgtg ctgcgccgtc ttccgggcgt
    25681 cgatatcacc caaaacggcg gttcaggtca gctctcatct atttttattc gcggtacaaa
    25741 tgccagtcat gtgttggtgt taattgatgg cgtacgcctg aatctggcgg gggtgagtgg
    25801 ttctgccgac cttagccagt tccctattgc gcttgtccag cgtgttgaat atatccgtgg
    25861 gccacgctcc gccgtttatg gttccgatgc aataggcggg gtggtgaata tcatcacgac
    25921 gcgcgatgaa cccggaacgg aaatttcagc agggtgggga agcaatagtt atcagaacta
    25981 tgatgtctct acgcagcaac aactggggga taagacacgg gtaacgctgt tgggcgatta
    26041 tgcccatact catggttatg atgttgttgc ctatggtaat accggaacgc aagcgcagac
    26101 agataacgat ggttttttaa gtaaaacgct ttatggcgcg ctggagcata actttactga
    26161 tgcctggagc ggctttgtgc gcggctatgg ctatgataac cgtaccaatt atgacgcgta
    26221 ttattctccc ggttcaccgt tgctcgatac ccgtaaactc tatagccaaa gttgggacgc
    26281 cgggctgcgc tataacggcg aactgattaa atcacaactc attaccagct atagccatag
    26341 caaagattac aactacgatc cccattatgg tcgttatgat tcgtcggcga cgctcgatga
    26401 gatgaagcaa tacaccgtcc agtgggcaaa caatgtcatc gttggtcacg gtagtattgg
    26461 tgcgggtgtc gactggcaga aacagactac gacgccgggt acaggttatg ttgagaatgg
    26521 atatgatcaa cgtaataccg gcatctatct gaccgggctg caacaagtcg gcgattttac
    26581 ctttgaaggc gcagcacgca gtgacgataa ctcacagttt ggtcgtcatg gaacctggca
    26641 aaccagcgcc ggttgggaat tcatcgaagg ttatcgcttc attgcttcct acgggacatc
    26701 ttataaggca ccaaatctgg ggcaactgta tggcttctac ggaaatccga atctggaccc
    26761 ggagaaaagc aaacagtggg aaggcgcgtt tgaaggctta accgctgggg tgaactggcg
    26821 tatttccgga tatcgtaacg atgtcagtga cttgatcgat tatgatgatc acaccctgaa
    26881 atattacaac gaagggaaag cgcggattaa gggcgtcgag gcgaccgcca attttgatac
    26941 cggaccactg acgcatactg tgagttatga ttatgtcgat gcgcgcaatg cgattaccga
    27001 cacgccgttg ttacgccgtg ctaaacagca ggtgaaatac cagctcgact ggcagttgta
    27061 tgacttcgac tggggtatta cttatcagta tttaggcact cgctatgata aggattactc
    27121 atcttatcct tatcaaaccg ttaaaatggg cggtgtgagc ttgtgggatc ttgcggttgc
    27181 gtatccggtc acctctcatc tgacagttcg tggtaaaata gccaacctgt tcgacaaaga
    27241 ttatgagaca gtctatggct accaaactgc aggacgggaa tacaccttgt ctggcagcta
    27301 caccttctga accacgtccc accgtgctgg tgtttgactc cggcgtcggt gggttgtcgg
    27361 tctatgacga gatccggcat ctcttaccgg atctccatta catttatgct ttcgataacg
    27421 tcgcttttcc gtatggcgaa aaaagcgaag agtttattgt tgagcgagtg gtggcaattg
    27481 tcaccgcagt gcaagaacgt tatccccttg cgctggctgt ggtcgcttgc aacaccgcca
    27541 gtaccgtttc acttcctgca ttacgcgaaa agttcgactt cccggttgtt ggtgtcgtgc
    27601 cggcgattaa acctgctgca cgtctgacgg caaatggcat tgtcggatta ctggcaaccc
    27661 gcggaactgt taaacgttct tatactcatg agctgatcgc gcgtttcgct aatgaatgcc
    27721 agatagaaat gctgggctcg gcagagatgg ttgagttggc tgaagcgaag cttcatggcg
    27781 aagatgtttc tctggatgca ctaaaacgta tcctacgccc gtggttaaga atgaaagagc
    27841 cgccagatac cgttgtattg ggttgcaccc atttccctct actacaagaa gaactcttac
    27901 aggtgctgcc agagggaacc cggctggtgg attctggcgc agcgattgct cgcagaacgg
    27961 cctggctgtt agaacatgaa gctccggatg caaaatctgc cgatgcgaat attgcctttt
    28021 gtatggcaat gacgccagaa gctgaacaat tattgcccgt tttacagcgt tacggcttcg
    28081 aaacgctcga aaaactggca gttttaggct gatttggttg aatgttgcgc ggtcagaaaa
    28141 ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc accactgaca
    28201 cggaacaacg gcaaacacgt cgccgggtca gcggggttct cctgagaatc tcaacagaga
    28261 aaagcaaaaa atgcttgact ctgtagcggg aaagcgtatt atgcacaccc cgcgacgctg
    28321 agaaaaagcg aagcggcact gctctttaac aatttatcag acaatctgtg tgggcactcg
    28381 aagatacgga ttcttaacgt cgcaagacga aaaatgaata ccaagtctca agagtgaaca
    28441 cgtaattcat tacgaagttt aattctttga gcatcaaact tttaaattga agagtttgat
    28501 catggctcag attgaacgct ggcggcaggc ctaacacatg caagtcgaac ggtaacagga
    28561 agcagcttgc tg
//
LOCUS       DAZGJW010000057        28265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZGJW010000057 DAZGJW010000000
VERSION     DAZGJW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.057"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..698
                     /gene="bcsQ"
                     /locus_tag="ACHKIK_002416"
     CDS             <1..698
                     /gene="bcsQ"
                     /locus_tag="ACHKIK_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO9083575.1"
                     /translation="AALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWARAML
                     DGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQLKASGRYQWI
                     LIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINNFRIGSQV
                     QDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLANWCLL
                     NYSGLKTPVGSAS"
     gene            695..3313
                     /gene="bcsA"
                     /locus_tag="ACHKIK_002417"
     CDS             695..3313
                     /gene="bcsA"
                     /locus_tag="ACHKIK_002417"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO9083576.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            3324..5663
                     /gene="bcsB"
                     /locus_tag="ACHKIK_002418"
     CDS             3324..5663
                     /gene="bcsB"
                     /locus_tag="ACHKIK_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO9083577.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            5670..6776
                     /gene="bcsZ"
                     /locus_tag="ACHKIK_002419"
     CDS             5670..6776
                     /gene="bcsZ"
                     /locus_tag="ACHKIK_002419"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO9083578.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDIWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            6758..10231
                     /gene="bcsC"
                     /locus_tag="ACHKIK_002420"
     CDS             6758..10231
                     /gene="bcsC"
                     /locus_tag="ACHKIK_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO9083579.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTLEPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            10313..12301
                     /gene="hmsP"
                     /locus_tag="ACHKIK_002421"
     CDS             10313..12301
                     /gene="hmsP"
                     /locus_tag="ACHKIK_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO9083580.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSMLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12484..13770
                     /gene="dctA"
                     /locus_tag="ACHKIK_002422"
     CDS             12484..13770
                     /gene="dctA"
                     /locus_tag="ACHKIK_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO9083581.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13990..15486
                     /locus_tag="ACHKIK_002423"
     CDS             13990..15486
                     /locus_tag="ACHKIK_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO9083582.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(15582..16511)
                     /gene="kdgK"
                     /locus_tag="ACHKIK_002424"
     CDS             complement(15582..16511)
                     /gene="kdgK"
                     /locus_tag="ACHKIK_002424"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO9083583.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16743..17510
                     /gene="pdeH"
                     /locus_tag="ACHKIK_002425"
     CDS             16743..17510
                     /gene="pdeH"
                     /locus_tag="ACHKIK_002425"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO9083584.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            17580..19640
                     /locus_tag="ACHKIK_002426"
     CDS             17580..19640
                     /locus_tag="ACHKIK_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO9083585.1"
                     /translation="MSKAGKITAAISVAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19874..21196)
                     /locus_tag="ACHKIK_002427"
     CDS             complement(19874..21196)
                     /locus_tag="ACHKIK_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9083586.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21607..22620)
                     /gene="yhjD"
                     /locus_tag="ACHKIK_002428"
     CDS             complement(21607..22620)
                     /gene="yhjD"
                     /locus_tag="ACHKIK_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO9083587.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22669..23568)
                     /locus_tag="ACHKIK_002429"
     CDS             complement(22669..23568)
                     /locus_tag="ACHKIK_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9083588.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            24088..24690
                     /locus_tag="ACHKIK_002430"
     CDS             24088..24690
                     /locus_tag="ACHKIK_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO9083589.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(24741..26390)
                     /locus_tag="ACHKIK_002431"
     CDS             complement(24741..26390)
                     /locus_tag="ACHKIK_002431"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO9083590.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            26795..28192
                     /gene="ccp"
                     /locus_tag="ACHKIK_002432"
     CDS             26795..28192
                     /gene="ccp"
                     /locus_tag="ACHKIK_002432"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO9083591.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT         6636 a         7163 c         7843 g         6623 t
ORIGIN      
        1 ccgccgcatt agcctggtca ttacaaatgt tgggagaaaa tgttctggtg gtcgatgcct
       61 gcccggacaa cttgttgcgc ctgtcattta atgttgattt tacccaccgt cagggctggg
      121 ccagagcgat gctggatggc caggactggc gtgacgctgg gttgcgctac acctcgcagc
      181 tcgatttgct gccttttggt cagttatcca ttgaagaaca agaaaatcca cagcactggc
      241 aaacccggct gagcgatatt tgctccggct tacagcaact aaaagccagc gggcgttacc
      301 agtggatttt aatcgactta ccgcgtgatg cctcgcagat aacccaccag ctgctgagtt
      361 tgtgcgatca ctcgctggct atcgtcaatg tggatgccaa ctgccatatc cgcctgcatc
      421 agcaagcgct gccggatggc gcacatattt tgattaataa cttccgtatt ggcagtcagg
      481 ttcaggacga tatttaccag ctttggttgc aaagccagcg ccgattgctg ccgatgctca
      541 ttcatcgtga tgaagcgatg gctgaatgcc tggcggctaa gcaaccagtc ggtgaatacc
      601 gcagtgatgc gctggcggca gaagagatac tgacgctggc gaactggtgc ctgttgaact
      661 attccgggct gaaaacgcca gtcgggagtg catcatgagt atcctgaccc ggtggttgct
      721 tatcccgccg gtcaacgcgc ggcttatcgg gcgttatcgc gattatcgtc gccacggtgc
      781 gtcggctttc agcgcgacgc tcggctgttt ctggatgatc ctggcctgga tttttattcc
      841 gctggagcac ccgcgctggc agcgtattcg cgcagaacat aaaaacctgt atccgcatat
      901 caacgcctcg cgtccgcgtc cgctggaccc ggtccgttat ctcattcaaa catgctggtt
      961 attgatcggt gcatcgcgca aagaaacgcc gaaaccgcgc aggcgggcat tttcaggtct
     1021 gcaaaatatt cgtggacgtt accatcaatg gatgaacgag ctgcctgagc gcgttagcca
     1081 taaaacacag catctggatg agaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg
     1141 gttgatcctc ggtatcatcg tcaccttctc gctgattctg gcgttaatct gcgttactca
     1201 gccgtttaac ccgctggcgc agtttatctt cctgatgctg ctgtgggggg tagcgctgat
     1261 cgtacggcgg atgccggggc gcttctcggc gctaatgttg attgtgctgt cgctgaccgt
     1321 ttcttgccgt tatatctggt ggcgttacac ctctacgctg aactgggacg atccggtcag
     1381 cctggtgtgc gggcttattc tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct
     1441 cggctacttc caggtagtat ggccgctgaa tcgtcagccg gtgccattgc cgaaagatat
     1501 gtcgctgtgg ccgtcggtgg atatctttgt cccgacttac aacgaagatc tcaacgtggt
     1561 gaaaaatact atttacgcct cgctgggtat cgactggccg aaagacaagc tgaacatctg
     1621 gatcctcgat gacggcggta gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa
     1681 atatatcgcc cgtaccactc atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa
     1741 atatgccaaa ggcgagttcg tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt
     1801 cctgcaaatg accatgggct ggttcctgaa agagaaacag ctggcgatga tgcagacgcc
     1861 gcaccacttc ttctcgccgg acccgtttga acgcaacctg gggcgttttc gtaaaacgcc
     1921 gaacgaaggc acgctgttct atggtctggt gcaggatggc aacgatatgt gggacgccac
     1981 tttcttctgc ggttcctgtg cagtaattcg tcgcaaacca ttggatgaaa ttggcggtat
     2041 tgctgtcgaa acggtaaccg aagatgcgca cacgtctctg cgcctgcacc gtcgtggcta
     2101 cacctctgca tatatgcgta ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc
     2161 gcatatcggt cagcgtattc gctgggcacg cgggatggtg caaatcttcc gtctcgataa
     2221 cccgcttacc ggtaaagggc tgaaattcgc ccagcggctg tgctacgtca acgccatgtt
     2281 ccacttcttg tcgggcattc cacgactgat cttcctgact gcgccgctgg cgttcctgct
     2341 gcttcatgcc tacatcatct atgcgccagc gttgatgatc gccctgttcg tgctgccgca
     2401 tatgatccat gccagcctga ccaactccaa gatccagggc aaatatcgcc actctttctg
     2461 gagtgaaatc tacgaaacgg tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct
     2521 gattaacccg cacaaaggca aatttaacgt caccgccaaa ggtggactgg tggaagaaga
     2581 gtacgtcgac tgggtgatct cgcggcccta catcttcctt gtcctgctca acctggtggg
     2641 cgttgcggta ggcatctggc gctacttcta tggcccgcca accgagatgc tcaccgtggt
     2701 cgtcagtatg gtgtgggtat tctacaacct gattgttctt ggcggcgcag ttgcagtatc
     2761 ggtagaaagc aaacaggtac gccgatcgca ccgcgtggag atgacgatgc ccgcggcaat
     2821 tgcccgcgaa gatggtcacc tcttctcgtg taccgttcag gatttctccg acggtggttt
     2881 ggggatcaag atcaacggtc aggcgcagat tctggaaggg cagaaagtga atctgttgct
     2941 taaacgcggt cagcaggaat acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga
     3001 agttgggctg aaattaatgc cgctcaccac ccagcaacat atcgattttg tgcagtgtac
     3061 gtttgcccgt gcggatacat gggcgctctg gcaggacagc tacccggaag ataagccgct
     3121 ggaaagtctg ctggatattc tgaagctcgg cttccgtggc taccgccatc tggcggagtt
     3181 tgcgccttct tcggtgaagg gcatattccg tgtgctgact tctctggttt cctgggttgt
     3241 atcgtttatt ccgcgccgcc cggagcggag cgaaacggca caaccatcgg atcaggcttt
     3301 ggctcaacaa tgatgataac gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta
     3361 tggggatgag tgcgttcccc tctttcatga cgcaggcgac gccagcaacg caaccactga
     3421 tcaatgctga gccagctgta gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga
     3481 tgccgggcgt gcagggcgct gatgcgccag tcgtggcgca gaacggtcct tcgcgtgatg
     3541 tgaagctgac ctttgcgcaa attgcgccgc cgccgggcag catggtgcta cgtggcatta
     3601 acccgaacgg cagcattgag tttggcatgc gcagcgatga agtggtgacg aaggcgatgc
     3661 tcaacctcga atacacgcca tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt
     3721 atctcaatga tgagctgatg ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa
     3781 cgctggcgca aatgcccatt aacccactgt ttattaccga cttcaaccgt gtgcggctgg
     3841 agtttgtcgg ccattatcag gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg
     3901 atgttgggcg gagcagtgga ctggatctga cctatcagac cctgaatgtg aagaatgacc
     3961 tgtcacactt cccggtgcca ttctttgacc cgcgcgataa ccgtactaac accttgccga
     4021 tggtctttgc gggtgcgccg gatgttgggc tgcaacaagc ctctgccatt gtcgcctcgt
     4081 ggtttggttc gcgttctggc tggcgtgggc agaacttccc ggtgctctat aaccaactgc
     4141 cggatcgtaa tgccattgtc tttgcaacca acgacaaacg gccggacttc ctgcgcgatc
     4201 atccggcggt aaaagcccct gtgattgaga tgattaacca tccgcagaat ccttacgtca
     4261 aactgctggt ggtgtttggt cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg
     4321 ctcagggtaa cattctgttc cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc
     4381 taccgcgtaa gccgtacgat gcgccgaact gggtacgtac cgatcgtccg gtcacatttg
     4441 gcgaactgaa aacctatgaa gaacagttac aatccagcgg tcttgagcca gcagcgatta
     4501 acgtttcgct aaacctgccg ccggatctct acctgatgcg cagtaccggc attgatatgg
     4561 atattaatta ccgctacacc atgccgccgg tgaaagacag ttcgcggatg gatatcagcc
     4621 tgaataacca gttcctgcaa tccttcaatc tgagcagcaa acaggaggcg aaccgcctgc
     4681 tgctgcggat tccggtatta caaggtttgc tggatggcaa aacagatgtc tctattccgg
     4741 cgctgaaact gggcgcgacc aaccagctgc gcttcgactt tgagtatatg aacccgatgc
     4801 cgggcggttc ggtggataac tgtattacct tccagccggt gcagaatcat gtggtgattg
     4861 gtgacgactc caccatcgac ttctcgaagt attaccactt catcccgatg ccggatctac
     4921 gcgcctttgc taacgcgggc ttcccattca gccggatggc ggatctgtcg caaaccatca
     4981 ccgtgatgcc gaaagcgcct aacgaagcac agatggaaac gttgctgaat actgttggtt
     5041 ttatcggcgc acagacgggc ttcccggcga ttaatctgac ggtgaccgat gatggcagca
     5101 ccattcaggg caaagatgcc gacatcatga tcatcggtgg tatcccggac aaactgaaag
     5161 acgataagca gatcgaccta ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc
     5221 gccagacccc gttccccggc attgtgccgg acgagagcga tcgcgcggca gaaacccggt
     5281 caacgctgac ctcttccggt gcgatggcgg cggtgattgg cttccagtcg ccgtataacg
     5341 accagcgcag cgtgattgcg ctgttggcag atagcccacg cggttatgaa atgcttaacg
     5401 atgcggtgaa cgatagcggc aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg
     5461 agtccggtat caacagccta cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt
     5521 tcgagcgcgt gtggtatgcg ctggcaaacc atccgattct gctggcggtg ctggcggcta
     5581 tcagtgtgat attgctggca tgggtactgt ggcgtctgct gcgaattatt agtcgtcgtc
     5641 gtcttaaccc ggataacgag taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga
     5701 tgctgctgct ggctgccttt agtgttcagg cagcctgtac ctggcctgcc tgggagcagt
     5761 ttaaaaagga ttacatcagt caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa
     5821 tcaccacctc cgaagggcaa agttacggca tgttctttgc cctggcggct aacgaccgtg
     5881 cagctttcga taatattctc gactggacgc agaacaatct cgctcagggt tctttaaaag
     5941 aacgtttgcc cgcctggctg tggggcaaga aagagaacag taagtgggaa gtgctggaca
     6001 gcaattcggc ctccgatggt gatatctgga tggcctggtc gttgctggag gcggggcgtt
     6061 tgtggaaaga gcagcgttat accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg
     6121 aggaagtggt gacggtgcct gggctgggtt ccatgttgtt accgggcaaa gtgggttttg
     6181 ctgaggataa cagctggcgt tttaacccca gctacctgcc gccgacgctg gcgcagtatt
     6241 tcacccgctt tggcgcgccg tggaccacgc tgcgcgaaac caatcaacgt ttattgctgg
     6301 aaaccgcccc gaaaggtttt tcgccagact gggtgcgcta tgagaaagac aaaggctggc
     6361 agctaaaagc cgaaaaaaca ttgatcagca gctacgacgc tatccgcgtt tacatgtggg
     6421 taggcatgat gcctgacagc gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga
     6481 tggcgacatt cactgagaaa aacggttatc cgccggaaaa agtggatgtg gctacgggga
     6541 aagcgcaggg taaaggaccg gtcggttttt ctgccgccat gctgcccttt ttacaaaacc
     6601 gcgatgcgca ggccgttcag cgccagcgcg tggccgataa ctttcccggc agcgatgcct
     6661 attacaacta tgtgctgacc ctgtttggac aaggctggga tcaacaccgt ttccgcttct
     6721 cgacaaaagg tgagttatta cctgactggg gccaggaatg cgcaaattca cactaaacat
     6781 attcacgctt tccctcggtc tggccgtcat gccgatggtc gaggcagcac caaccgctca
     6841 gcaacagttg ctggagcaag ttcggttagg cgaagcgacc catcgtgaag atctggtgca
     6901 acagtcgtta tatcggctgg aacttattga tccgaataac ccggacgtcg ttgccgcccg
     6961 tttccgttct ttgttacgtc agggcgatat tgatggcgcg caaaaacagc tcgatcggct
     7021 gtcgcagtta gcgccgagtt caaatgcgta taaatcgtcg cggactacga tgctactttc
     7081 cacgccggat ggtcgtcagg cactgcaaca ggcacgattg caggcgacga ccggtcatgc
     7141 agaagaagct gtggcgagtt acaacaaact gttcaacggt gcgccgccgg aaggtgacat
     7201 tgctgtcgag tactggagta cggtggcgaa aattccggct cgccgtggcg aagcgattaa
     7261 tcagttaaaa cgcatcaatg cggatgcacc gggcaatacg ggcctgcaaa acaatctggc
     7321 gctattgctg tttagtagcg atcgccgtga cgaaggtttt gccgtcctgg aacagatggc
     7381 aaaatcgaac gccgggcgcg aaggggcctc taaaatctgg tacgggcaga ttaaagacat
     7441 gcccgtcagt gatgccagtg tgtcggcgct gaaaaaatat ctctcgatct ttagtgatgg
     7501 cgatagcgtg gcggctgcgc aatcgcaact ggcagaacag caaaaacagc tggccgatcc
     7561 tgctttccgc gctcgtgcgc aaggtttagc ggcggtggac tctggtatgg cgggtaaagc
     7621 cattcccgaa ctacaacagg cggtgcgggc gaacccgaaa gacagtgaag ctctgggggc
     7681 gctgggccag gcgtattctc agaaaggcga tcgcgccaat gcagtggcga atctggaaaa
     7741 agccctcgca ctggacccgc acagcagcaa caacgacaaa tggaacagtc tgctgaaagt
     7801 aaaccgctac tggctggcga tccagcaggg cgatgctgcg ctgaaagcca ataatcctga
     7861 ccgggcagaa cgcctgttcc agcaggcgcg taatgtcgat aacaccgaca gttatgcagt
     7921 gctggggctg ggcgatgtgg cgatggcgcg aaaagattat cccgccgccg aacgttatta
     7981 tcagcagacc ttgcgtatgg acagcggcaa cactaacgcc gtgcgcgggc tggcaaatat
     8041 ttaccgccag caatcgccag aaaaagctga agcgtttatc gcctcgctct ctgccagtca
     8101 gcggcgtagc attgatgata tcgaacgcag cctgcaaaac gaccgtctgg cacagcaggc
     8161 agaggcactg gaaaaccagg gcaaatgggc gcaggcggca gcacttcagc ggcaacgact
     8221 ggcgctggac cccggcagcg tatggattac ttaccgactt tcgcaggatc tctggcaggc
     8281 cggacaacgc agccaggccg atacgttaat gcgcaatctg gcgcagcaga agccgaacga
     8341 cccggagcag gtttacgctt acgggctgta cctctctggt catgaccagg acagagcggc
     8401 gctggcgcat atcaacagcc tgccgcgcgc gcagtggaac agcaatattc aggagctggt
     8461 taatcgactg caaagcgatc aggtgctgga aaccgctaat cgcctgcgag aaagcggcaa
     8521 agaagctgaa gcggaagcga tgctgcgtca gcaaccacct tccacgcgta ttgacctcac
     8581 gctggctgac tgggcgcagc agcgacgtga ttacaccgcc gcccgcgctg catatcagaa
     8641 tgtcctgacg ctggagccaa ctaacgccga tgccattctg gggctgacgg aagtggatat
     8701 tgctgccggt gacacagcgg cggcacgtag ccagctggcg aaactgcccg ctaccgataa
     8761 cgcctcgctg aacacacagc ggcgcgtggc gctggcgcag gcgcagcttg gagataccgc
     8821 agcggcgcag cagacgttta ataagttgat cccgcaggca aaatctcagc caccgtcgat
     8881 ggaaagcgcg atggtgctgc gcgacggtgc gaagtttgaa gcgcaggcgg gcgatccaaa
     8941 gcaggcgctg gaaacctaca aagacgccat ggtcgcatcc ggtgtgacta cgacgcgtcc
     9001 gcaggataac gacacattca cccgactgac ccgtaacgac gagaaagatg actggctgaa
     9061 acgcggcgtg cgcagcgatg cggcggacct ctatcgccag caggatctta acgtcaccct
     9121 cgagcacgat tactggggtt cgagcggcac cggtggttac tccgatctga aagcgcacac
     9181 taccatgttg caggtggatg cgccgtattc tgacgggcgg atgttctttc gcagtgattt
     9241 cgtcaatatg aacgtcggca gtttctccac taatgccgat ggcaaatggg atgacaactg
     9301 gggcacctgt acattacagg actgtagcgg caaccgcagc cagtcggatt ccggtgccag
     9361 cgtggcggtc ggctggcgaa atgacgtctg gagctgggat atcggtacca cgccgatggg
     9421 cttcaacgtg gtggatgtgg tcggcggcat cagttacagc gatgatatcg ggccgctggg
     9481 ttacaccgtt aacgcccacc gtcggcccat ctccagttct ttgctggcct ttggtgggca
     9541 aaaagactcc ccgagcaata ccgggaaaaa atggggtggc gtacgtgccg acggtgtggg
     9601 gctaagtctg agctacgata aaggtgaagc aaacggcgtc tgggcatcgc ttagtggcga
     9661 ccagttaacc ggtaaaaatg tcgaagataa ctggcgcgtg cgctggatga cgggctatta
     9721 ctataaggtc attaaccaga acaatcgccg cgtcacaatc ggcctgaaca acatgatctg
     9781 gcattacgac aaagatctga gtggctactc actcggtcag ggcggttact acagtccgca
     9841 ggaatacctg tcgtttgcca taccggtgat gtggcgggag cgcacggaaa actggtcgtg
     9901 ggagctgggg gcgtctggct cgtggtcgca ttcacgcacc aaaaccatgc cgcgttatcc
     9961 gctgatgaat ctgatcccga ccgactggca ggaagaagct gcgcggcaat ccaacgatgg
    10021 cggcagcagt cagggcttcg gctacacggc gcgggcatta cttgaacgac gtgttacttc
    10081 caactggttt gttggcacgg caattgatat ccagcaggcg aaagattacg cacccagcca
    10141 tttcctgctc tacgtacgtt attccgccgc cggatggcag ggtgacatgg atttaccgcc
    10201 gcagccgctg ataccttacg ccgactggta agttttcaga tagcgcctct cttaatgccg
    10261 ctgcgatcgg gtatactcgg gcggcaatct gggatttccg gggggagaca atttgcgcgt
    10321 aagtcgctcg ttaacaatca agcagatggc aatggtggca gccgttgtcc tggtgttcgt
    10381 ttttattttt tgcaccgttt tgctgttcca tctggtccaa cagaatcgct ataacacggc
    10441 tacgcaactg gaaagcattg ctcgctctgt ccgcgaaccc ttatcttcag ctattttgaa
    10501 aggcgatatt cccgaagcgg aagctattct tgccagcatt aaaccggcag gcgtggtcag
    10561 ccgtgccgat gtagtgctgc ctaaccagtt ccaggcgctg cgtaaaagtt ttattccaga
    10621 gcgcccggtg ccggtaatgg ttactcgcct gtttgagcta ccggttcaaa tctcgctggg
    10681 cgtttactcg ctcgaacgtc cggcaaaccc gcagccaatt gcctatctgg tactacaggc
    10741 ggattccttc cgtatgtata agttcgtgat gagcaccctc tcaatgttag tgaccattta
    10801 cttacttttg tcgcttatcc tgaccgtcgc catcagctgg tgcattaacc gcctgatttt
    10861 gcatccgtta cgcaatattg ctcgcgaact taacgccatc ccagcccagg agcttgttgg
    10921 tcaccaactg gcattaccgc gtctgcatca ggacgatgaa atcggtatgt tggtgcgcag
    10981 ttacaacctc aaccagcaat tgctgcagcg ccattatgaa gaacagaacg aaaatgcgat
    11041 gcgcttcccg gtgtcggatt tgccgaacaa agccttgctg atggagatgc tggagcaggt
    11101 tgtcgcgcgt aaacaaacca ccgcgctgat gatcatcacc tgtgaaaccc tgcgtgatac
    11161 tgcgggcgtg ctgaaagagg cgcaacgaga aattctgctg ctgacgctgg tggaaaaact
    11221 caaatcggta ctgtcgccac gtatgatcct cgcgcagatt agcggttatg actttgctgt
    11281 cattgccaac ggtgtacagg aaccgtggca cgcaatcacc ttaggtcagc aagtgctcac
    11341 tatcatgagc gagcgcctgc cgattgaacg tattcaactc cgtccgcact gtagcattgg
    11401 cgtggcgatg ttctacggcg atctcaccgc cgaacagctt tacagtcgcg ctatttctgc
    11461 ggcatttacc gctcgccata aaggcaagaa tcagattcag ttctttgatc cgcagcagat
    11521 ggaagccgcc cagaagcggt tgacggaaga gagcgatatc cttaatgcac tggaaaatca
    11581 tcagtttgct atttggttac agccacaggt cgagatgacc agcggaaaac tggtcagtgc
    11641 ggaagtgtta ctgcgtatcc agcaaccgga tggcagttgg gacctgccgg atggcttaat
    11701 cgatcgcatt gagtgctgtg ggctgatggt taccgtcggt cactgggtgc tggaagagtc
    11761 ctgtcgattg cttgcagcct ggcaagagcg cggcattatg ctgcccttgt cggtaaacct
    11821 ctctgcgctg caactgatgc acccaaatat ggtggcggat atgctggaac tgttaacccg
    11881 ctatcgcatt cagccgggaa cactgattct ggaagtgaca gaaagccgac gtattgacga
    11941 ccctcatgct gcggtggcaa tcctccgtcc gctgcgcaat gccggagttc gggtggcgct
    12001 ggatgatttc ggcatgggct acgcagggct gcgtcagctg cagcatatga aatcgttgcc
    12061 aatcgacgta ctgaaaatcg acaaaatgtt tgttgaaggc ttgccggaag atagcagcat
    12121 gattgctgca attatcatgc tggcgcagag cctgaactta caaatgattg ccgaaggcgt
    12181 ggagactgaa gcacaacgcg actggctggc aaaagcgggc gttggtattg cccagggctt
    12241 cctttttgct cgcccactcc ctattgaaat cttcgaagag agttacctgg aagaaaagta
    12301 gctaccccaa actgattaca aaactttaaa aagtgctggt ttgtgcgagc cagctcaaac
    12361 tttttaacct ttttgtttca attatgatcc aggtacattt ctgtgatgtt gtctgggtgt
    12421 tattttaagg ccgcaggtac cccataacct tacaagacct gtggttttac taaaggacac
    12481 cctatgaaaa cctctctgtt taaaagcctt tactttcagg tcctgacagc gatagccatt
    12541 ggtattctcc ttggccattt ctatcctgaa ataggcgagc aaatgaaacc gcttggcgac
    12601 ggcttcgtta agctcattaa gatgatcatc gctcctgtca tcttttgtac cgtcgtaacg
    12661 ggcattgcgg gcatggaaag catgaaggcg gtcggtcgta ccggcgcagt cgcactgctt
    12721 tactttgaaa ttgtcagtac catcgcgctg attattggtc ttatcatcgt taacgtcgtg
    12781 cagcctggtg ccggaatgaa cgtcgatccg gcaacgcttg atgcgaaagc ggtagcggtt
    12841 tacgccgatc aggcgaaaga ccagggcatt gtcgccttca ttatggatgt catcccggcg
    12901 agcgtcattg gcgcatttgc cagcggtaac attctgcagg tgctgctgtt tgccgtactg
    12961 tttggttttg cgctccaccg tctgggcagc aaaggccaac tgatttttaa cgtcatcgaa
    13021 agtttctcgc aggtcatctt cggcatcatc aatatgatca tgcgtctggc gcctattggt
    13081 gcgttcgggg caatggcgtt taccatcggt aaatacggcg tcggcacact ggtgcaactg
    13141 gggcagctga ttatctgttt ctacattacc tgtatcctgt ttgtggtgct ggtattgggt
    13201 tcaatcgcta aagcgactgg tttcagtatc ttcaaattta tccgctacat ccgtgaagaa
    13261 ctgctgattg tactggggac ttcatcttcc gagtcggcgc tgccgcgtat gctcgacaag
    13321 atggagaaac tcggctgccg caaatcggtg gtggggctgg tcatcccgac aggctactcg
    13381 tttaaccttg atggcacatc gatatacctg acaatggcgg cggtgtttat cgcccaggcc
    13441 actaacagtc agatggatat cgtccaccaa atcacgctgt taatcgtgtt gctgctttct
    13501 tctaaagggg cggcaggggt aacgggtagt ggctttatcg tgctggcggc gacgctctct
    13561 gcggtgggcc atttgccggt agcgggtctg gcgctgatcc tcggtatcga ccgctttatg
    13621 tcagaagctc gtgcgctgac taacctggtc ggtaacggcg tagcgaccat tgtcgttgct
    13681 aagtgggtga aagaactgga ccacaaaaaa ctggacgatg tgctgaataa tcgtgcgccg
    13741 gatggcaaaa cgcacgaatt atcctcttaa tctcatcact tatgcccgca ctcccttccc
    13801 cgagtgcggg tgtttgcgca taattgaccc ctgtgcctga acttaccacc tgggtgagtt
    13861 caggtttatt tcacttcttc cgtgacattt tcatgttctt gcggtctaac acgaagtgtt
    13921 tttaacgtca tttagactga ccgacaacgg gcagtttttt attaccagga tagttgatca
    13981 ggggttcaca tgcagggcac aaaaattcga cttttagcgg gcggtttgct gatgatggcc
    14041 actgctggct atgtgcaggc agatgcgctc cagcctgatc cagcatggca acaggggacg
    14101 ctttccaacg gtttacagtg gcaagtgctg accacccccc agcgtcccag cgatcgtgtt
    14161 gaaattcgcc tgctggttaa taccggttcg ctcgccgaaa gtacacaaca gagcggttac
    14221 agtcacgcca tccctcgtat tgcgctaacg caaagcggtg gccttgacgc agcacaggcg
    14281 cgttcattgt ggcagcaggg gatcgaccct aaacgcccga tgccgccggt aattgtctct
    14341 tatgacacca cgctgtttaa tctgagtttg cccaataacc gtaacgattt gctgaaagaa
    14401 gcgctctctt atctggcaaa tgccactggc aaattgacca tcacaccaga aaccatcaac
    14461 cacgcgctgc aaagtcagga catggtggca acctggcctg ccgatactaa agagggctgg
    14521 tggcgctatc gtctgaaagg gtcaaccttg ttaggtcacg atcctgccga tccgctgaaa
    14581 caacccgttg aagcggaaaa aattaaagat ttctatcaga aatggtacac cccggatgca
    14641 atgacgctac tggtggtggg aaacgtggat gcgcgctcgg ttgtcgacca aataaacaaa
    14701 acgtttggcg aactgaaagg caaacgtgaa acgccagctc cggtgccgac gctttctccg
    14761 ctgcgtgcgg aagcggtgag tattatgact gacgcggtgc gtcaggaccg gttatctatc
    14821 atgtgggata cgccgtggca gccgattcgt gaatcagccg cactgctgcg ctactggcgt
    14881 gcggacctgg cccgcgaggc gctgttctgg catgttcagc aagcgttaag cgccagtaac
    14941 agcaaagaca tcggtcttgg atttgactgc cgtgtgctgt atctgcgtgc gcagtgtgcc
    15001 atcaacatcg aatcaccaaa cgacaagctg aacagcaacc ttaatctggt ggcgcgtgaa
    15061 ctggcgaagg ttcgcgataa aggtctgccg gaagaagagt tcaatgcgtt agtggcgcaa
    15121 aagaaactgg agctgcagaa actgtttgcc gcctatgcgc gggctgatac cgatattctg
    15181 atgggtcagc ggatgcgttc gttgcaaaat caggtcgtcg atatcgcgcc ggagcagtat
    15241 cagaaactgc ggcaggattt ccttaacagc ctgacggtgg agatgttaaa tcaggatctg
    15301 cgtcagcagt tgtcgaatga tatggcgtta atactgctgc agccgaaagg cgagccggaa
    15361 tttaacatga aagcgttgca ggcggcctgg gatcaaatca tggccccatc gactgctgct
    15421 gccgccacct ctgtcgccac ggatgacgta catcctgaag tgacggatat tccacctgca
    15481 cagtaatgcc tgatggtgat gagttagcac aaccatcagg tatttatgga tgagctggat
    15541 agaggggtaa cactttatcc agccttttgc atatgctgcg tttacgctgg catcgcctca
    15601 cgcgggataa tcgcgccgcg atactgaata acggtacttg cggtcaggtg cccgcgtttc
    15661 gccgcgtttt ccgcactgcc gcccgtcaaa cgtaccgcca gataaccggc gctgaaagag
    15721 tcgccagctg cggtggtatc gatcactttt tctttcggca gtttcaccgc cggaacatcc
    15781 actaaccctt cgccagcaat ggacaccagg caagaatccg ccccgcgttt caccaccact
    15841 tctttcacgc ccgcgttatg ggtgcgcgca atgacgtctt ccaccggctg ttgaccccac
    15901 agcgcgtctt catcgtccag cgtcaggaag gcgatatccg tgcattccag catttgttgg
    15961 tacacctgct gtgtctcttc tttgctggcc cacaggcgcg gacgatagtt attgtcgaaa
    16021 atcacttttc cgccgttggc gcggcattcg cgcagcaggg aaagcagctt ttcgcggctg
    16081 gtcgggctta agatcgccag gctaatcccg ctcaggtaga gataatcgaa attcgccagc
    16141 tcttcgcaaa tcgccgcaga ctgctcactc tccagccaga atttggcggc ggcttcgttc
    16201 cgccagtagt agaacgtacg ctcgccggtg ctgtcggttt caatgtagta aaggcccggc
    16261 agacggtttt ccatccgttg ggtcagggaa gtatcaacgt tctcgccgtg ccaggcgtcc
    16321 agcatttgct ggctaaaact gtccgttccc agcgccgtta cgtaatgaac ggttaatgcc
    16381 gcaggatcga cctgacgggc gatatagacg gaagtgttca gggtatcgcc gccgaaaccg
    16441 cgcttaacgt ccgcgccttt ctcggaaagc tcaatcatgc attcgccaat cacggcaatc
    16501 tttttggaca tagtcgtgaa cctgatctgt aaaaaattag cgttagtttg cgctgtggtg
    16561 gtttgctggt caaccatatt aaaacagtgt tccattattt ttttgagcca gaacgtattc
    16621 ctgaaagatt ccgttgtgga gtgaggaaat ttaccccggc caggcggtaa agttctgccc
    16681 ttgcgcgccg ataatctttg tcgagtccgg gcagcatcac ttttaaacac aggacatctt
    16741 tgatgataag gcaggttatc cagcgaataa gcaaccctga agcaagcatc gagagcttgc
    16801 aggaacggcg tttttggttg cagtgtgagc gtgcttacac ctggcagccg atctatcaaa
    16861 catgcggacg gttaatggcc gtggagctat taacggtggt cacgcatccc ttgaaccctt
    16921 cgcaacgcct gccgccggat cgctatttta ctgaaatcac cgtcagccat cggatggagg
    16981 ttgtgaaaga gcagattgat ttgctggcgc aaaaagccga cttctttata gagcacggcc
    17041 tgctggcatc ggtcaatatt gatggcccta cgctcatcgc cctgcgtcag caaccaaaaa
    17101 tcctgcgcca gattgagcgt cttccctggc tgcgtttcga actggtggag catatccgac
    17161 tgccgaaaga ttcaaccttt gcctcgatgt gtgaatttgg cccgctgtgg ctggatgatt
    17221 ttggtaccgg gatggcaaat ttctctgcac taagtgaagt gcgttatgac tacatcaaaa
    17281 tcgcgcgaga actgtttgtg atgctgcgtc agtcgccgga aggacgcaca ctcttttctc
    17341 agcttttaca tctaatgaat cgctattgtc gcggggtgat tgtcgagggc gtagaaacgc
    17401 cggaagagtg gcgtgatgtt cagaactcgc ccgcattcgc cgcacaaggc tggtttcttt
    17461 cacgcccggc accgatagaa acgctgaata cggcggttct ggcgctataa gctgcctcat
    17521 tttccgcctg gctggactat ctttaggact ggcacaggaa gaatatgagg aagcgaacga
    17581 tgagcaaggc aggcaaaata accgctgcga tttcagtggc tttcttgttg ttgattgtcg
    17641 tggcgatcat tttgattgca acttttgact ggaatcgact caaaccgacc atcaaccaga
    17701 aagtctctgc ggagttgaat cgtccgttcg ctatccgtgg cgatctgggc gtggtgtggg
    17761 agcggcaaaa acaagaaact ggctggcgca gctgggtgcc gtggccccat gtacacgcgg
    17821 aagacatcat tcttggcaat ccaccggata ttcccgaagt cacgatggtg catttgccac
    17881 gcgtagaggc aacgctggcc ccgctggcgc tgctgaccaa aacggtctgg ctgccgtgga
    17941 tcaagctcga aaagcccgac gcgcgcctga ttcgcctctc cgaaaagaac aataactgga
    18001 cgtttaatct cgccaacgat gataacaaag acgcgaatgc aaagccgtcg gcatggtcgt
    18061 ttcggctgga taatattctt ttcgatcaag ggcggatcgc cattgatgac aaagtaagca
    18121 aagcggatct ggagattttt gttgatccgt taggcaagcc gctgccgttc agcgaagtta
    18181 ctggatcgaa aggtaaagcg gataaagaaa aggtgggcga ttacgttttt ggcctgaagg
    18241 cgcagggacg ttataacggt gaaccgctca cgggtacggg aaaaataggc ggtatgctgg
    18301 cgctgcgtgg cgaagggacg ccgtttccgg tacaggctga tttccgctct ggtaacaccc
    18361 gtgttgcttt tgatggcgtc gtgaatgatc caatgaagat gggcggtgtc gatttacggc
    18421 ttaaattttc tggcgattca ctgggtgatc tctatgaact gacgggcgtt ctgctgcccg
    18481 ataccccgcc gtttgaaacg gatggtcggc tggtagcgaa aatcgacact gaaaaatcgt
    18541 cggtctttga ttatcgcggt tttaatgggc gaattggcga tagcgatatc cacggttctc
    18601 tggtctacac caccggaaag ccacgaccaa aactggaagg tgatgtcgag tcgcggcaat
    18661 tgcggctggc ggacctggga ccgttaattg gcgttgactc cgggaaaggg gcagaaaagt
    18721 cgaaacggtc tgaacagaag aagggcgaaa aaagcgttca gcctgcgggc aaagtgctgc
    18781 cttatgaccg cttcgaaacc gataaatggg acgttatgga tgccgatgtt cgcttcaaag
    18841 ggcggcgcat tgagcatggc agtagcctgc cgattagtga tctttctact catatcatcc
    18901 tcaaaaatgc tgacctgcgc ctgcaaccgc tgaaatttgg catggcgggc ggcagcattg
    18961 cggcgaatat tcatctggaa ggcgataaaa agccgatgca ggggcgggca gatattcagg
    19021 ctcgtcgact gaaactgaaa gaactgatgc ccgatgtgga actgatgcag aagacgctgg
    19081 gggaaatgaa cggtgacgcg gaactacgcg gtagcggtaa ctcggtggcg gcacttttag
    19141 gcaacagtaa cggcaacctg aaactgttga tgaatgacgg gctggtgagc cgcaacctga
    19201 tggagattgt tgggctgaat gtcggcaact acattgtcgg tgcgatattt ggtgatgatg
    19261 aggtgcgggt gaactgcgcg gcggcgaatc tggatattgc caacggcgtg gcgcgcccgc
    19321 agatctttgc tttcgatact gagaacgcgt tgattaacgt taccggcacg gcaagttttg
    19381 cttccgaaca gctggatttg actattgatc cggagagtaa aggaattcgg attatcacac
    19441 tgcgttcgcc gctgtatgtg cgtgggacgt ttaaaaatcc gcaggctggg gtgaaagccg
    19501 ggccgttgat tgcccgtggt gctgttgctg cggcactggc aacgctggta acaccggcgg
    19561 cggcgttact ggcactgatc tcaccttccg aaggggaggc taatcagtgt cggacgatat
    19621 tgtcgcagat gaagaagtga tgaggttgga ttgtgtttgc tggtgagttc gggagcagcg
    19681 tgttcccctc accctaaccc tctccccaaa ggggcgaggg gactgttttg tgcgtgttga
    19741 aacaacgagt accgcttttc cgttcacttt ctgggggagg gctgttatgt tcgcgatata
    19801 acatctcgct ccgcttttct ccctctccct ttcagggaga gggctggggt gagggtggct
    19861 gcccacacga tacttacaac gactgatgtc gcgtctcatg ggtcagcagc agggcgatta
    19921 acgtcaagcc agccatcgcc gccagatata accccaccgc acctaacccg tagttagcct
    19981 gcaaccaggc tgcgatatat ggtgcaacgg aagccccgag aatcgacgct acgttgtaag
    20041 agaacgatgc tccggtgtaa cgcacttctg tcgggaacag ctctggtaac agcgcgccca
    20101 tcggcccgaa ggtcagtccc atcagactta accccagcag caggaaggca aaaaccagaa
    20161 tcgggttgcc agaaccgagc agcgggttaa aggcgaacag cgcgaacagg atgatcagcg
    20221 tggtgatgat taccatgctt ttacggcgac caaaggcatc agccagtaat ccggcgactg
    20281 gcaccatcac gccaaaacca ataactgcca tcatcagcat ccacaacact tcgttacgcg
    20341 gcaggccaag cccaactggc gcggcggcgg tactgaaggt catagagtag accgtcatga
    20401 tgtaaaacag cgtataggtt gccagcataa tgaacgtacc cagtacggtt acgcgaacat
    20461 gtttggtcag cagcgtaccc agcgggatct tcacctgttt tttcgcttta gcgactttct
    20521 caaacaccgg cgactcatgc agcgacacgc gaacatacag gccgataatg accagcaccg
    20581 ccgagaagat aaatggcaca cgccagcccc agctcataaa ctgctcgtcg gtcagtagcc
    20641 aggaaagcag caagaaagtg ccgttggcaa agaagaagcc aatcggtgcg cccagctgtg
    20701 ggaaggagcc atacagtgca cgtttgcgcg gtggggcgtt ttcagtcgcc agcagcgccg
    20761 cgccgcccca ttcaccgcct aagcccagac cctgaccaaa tcgagccagc gccagcagca
    20821 gcggggcgaa aataccaatc gtggcatagc ccggcagcag accaatcacc acggtcgaaa
    20881 tccccatcgt tagcaacgag gcgaccagcg tcgctttacg cccaacgcga tcgccaaaat
    20941 gaccaaaaac ggcagagcca atggggcgcg cgacgaaggc gatggcgaag gtggcgagcg
    21001 actgtagcgt tgctgccgtt ggatcgccct gcgggaagaa gatatgcgga aacacaataa
    21061 cggccgcagt ggcgtaaatg taaaagtcga agaactcaat ggccgtgcca atgagagagg
    21121 cgacaaggac tttattacgc gagttgatcg gcgtgtattc ttgctcgtgg tcgagtgttg
    21181 tggctgttgc ttgcataatc ttttcttatt ttttggctaa cgaatagcca tattacgcac
    21241 agcaaaagtg atatttcaat gtgtaacaaa tcgcggaaat ggtcaaaaac caggcaaaaa
    21301 ggggataatg tttaggccag tgatttttct tctatgaaat gcttcacata attttataaa
    21361 tagtggataa aactggtttc aggttaaata aaagttacca actggattta tatgctgggg
    21421 ttatgagttg gaatgaaatt gtttgctccc aaatagatgg ctgaagaggc tctggggaag
    21481 agtttcaaga ggttatgtgc gcattatagc cggatgcgat gctggcgcat cttatccggc
    21541 ctacggggag ccaggccgaa taaaatcgta tgccggataa ggcgtttacg ccgcatccgg
    21601 cctattttaa ggctgcgttt tccctggcat tcgcgggtcg tctttatatt cggcggtggc
    21661 aatccacgcc gcgcaaaaca gcgtcaaacg ggcgaagaag tagaaaaatg ccatcagccc
    21721 cagcacggaa ccaaatgctg cgccagaggg ggatttcatc agcgatggca aggtgtaggt
    21781 catcacgatt ttaatcactt caaaaccaat agcagccaga aatgttccgc gaatcagggc
    21841 ttttttgcgc ggacggtggc gcggcaaccg ccagaagatc cagaagaaaa gcagatagtt
    21901 ggcgaagatg gaaatcgcca ggccaatcaa ccgccacgtc ggtttcagcc actcaatgct
    21961 gttcaggtgc agggcgctaa taatcatttg ctgcgccgaa ccggcaaccg aggtgatcga
    22021 cagcgtcaca atcagcgcaa ttaacaaacc aatcagtgaa ataaaatcac gcagatattt
    22081 tacccagaac ttctcctgat cttgcggcga gcgttcccag acatcgcgcg actgggcacg
    22141 aatcgcttca cgcaggttac ccatccagtt gatgccggaa taaagtgcca ccgccaggcc
    22201 gacaagccct acagtcgtac gctgctgaac ggcggtgttg atggtgtttt tcaacgtggc
    22261 ggctagcgtc ggatcgctga tgttttgcag aattttgtcg aagatatcct gtagcaacat
    22321 cggatgggag gccagcacaa agccccctgc ggcaaacgac accatcaaaa tcgggatcat
    22381 cgacaaaaat gagaaatagg tgatagccgc accaaactgg ttacccagcc gatcgttaaa
    22441 gcgttctgtc gcacgaatca ggtgcgcaat aaccggttgg cgttggactt tttcggcagt
    22501 ggtggtgacg gtttccagcg cctggctaac tttgccgcct ttctcgctat tatccaactg
    22561 tttaatcggc tcgtgctcca gatcctgggt gggacgtttg atctcgtttt cctgcgtcat
    22621 ccgctctttt gtccttctgt cgtggtcatt cttaaaaagt atagtcggtc agtccaggtg
    22681 ctctttcatt acgccagcca gccactgcat aaacaggttt acacgccggg aaagctcccg
    22741 acgctgcggg taaaccagcg aaagggagag cggctcggca cggtagcctg gcaacacttc
    22801 aataagccgc ccggcacgca gggcttcgcg cacggcaatg cgcgggatct gaataatccc
    22861 cagaccggca agaccggcgg cgagatagtt ttcgctactg tttaccgtca acatgccgcc
    22921 agacttaaac cactggacgc cattaacgct ggcaacctca aaacctaacg gatgtacacc
    22981 caggtgcggt gtataacgca ctattgcgtg tgaagtcaga tcatcggggc tttgcggata
    23041 accaaagcgc gtcagatagt gcggactggc acagttgacc atggtcagtt tgccgagggg
    23101 acgggcgata acgccatctt ccggtaacgc gccagtgcgt atcacgcaat caaaaccatc
    23161 atgaagaata tctaccggac ggtcatggct actcagttcc agctcaattc ccggatgcag
    23221 atagagaaat tccgacaggc gcggcagtaa caggcttttc gcgattcctg gcgggatgtc
    23281 gatgcgtaat ttaccgctga tactggtggc atcctgttga aacagaccgt ccagttcgct
    23341 gagattactc aacacatctt ttgctcgttg ataataggtc atgccttctg gcgtgagttt
    23401 gacccgtcgc gtggtgcgct ggagaagctg ggtgccaagt tgatgttcca gtgcctgtat
    23461 ctggcgcgaa acacttcctt ttggcaaagc aaagaaatcc gccgcgcggg aaaaactttc
    23521 cagctccgcg actttgatga acaactgcat tgcgtgaatt ttatccattt ctttgaccga
    23581 ttgttgttca cagcgaaaca gtaacgcgca tatcgcattg tttattgttt tttctaacat
    23641 ctaatgccgg gtttcacaat gttgtcatga cgaatgtggc gatttctcag caggaaaagg
    23701 gcggaatagc tttctgagaa aggtgaatcc ccctaaacag agcgtatttt tcagcgattc
    23761 cctttaaagt gaatagagga cgtgttggct atttgtgctt atttttgtct cattgccgtt
    23821 tgttattttt gcttgtggtc gcggagtttc atttttaatt gcatgcgtac taaccaccaa
    23881 tataaggaaa tcctcaaaat tgttttaaat agtgccctcg acatgtaatt tcaggcgttt
    23941 agctgtaact atttatatcg gtaactattt tctacgcgtc atctctgtag aatggccacc
    24001 gccaaatggc gaattcttat tttttctcgc gggtaatacc cttccactac ttaagtcttt
    24061 ttaacgtatt caggtggaga ttcagttatg caaatagtca tgtttgacag gcagtcaata
    24121 tttattcatg gaatgaaaat cagtttacag cagcgtattc caggagtgag tattcagggg
    24181 gccagtcagg cagacgagtt atggcaaaag ctggaaagtt accctgaagc cttagttatg
    24241 ctcgatggcg atcaggatgg tgagttttgc tactggttgc tgcaaaaaac cgtggtgcag
    24301 tttcctgagg ttaaggtgtt aatcacggcg acggattgca ataaacggtg gttacaggaa
    24361 gttatccatt ttaatgtgct ggccattgtg cctcgtgatt caaccgtcga gacatttgcg
    24421 ttggcggtaa atagtgcggc gatggggatg atgtttctgc ctggagactg gcggacgacg
    24481 ccggaaaagg acattaaaga tctcaaatcg ttaagcgccc gccagcggga gattttaacc
    24541 atgttagcgg cgggcgaatc aaataaggag atcggcagag cgctgaatat cagtaccgga
    24601 acggtaaaag cgcatctgga atccctgtat cgccgtctgg aagtgaaaaa tcgcacccag
    24661 gcggcaatga tgttaaatat ctcctcctga agaggataat tttacagcca gcaggaagtt
    24721 agcggctggc tgtaaaaata ttatggttcg ccgtacaaac caattaaacg gcgtaccaca
    24781 ccgttagtcc agccaaaccc atcctgcaac ggatactcgc caccgccgcc ttcgcgggga
    24841 acaccatcgg caatatggta tttctcgatc attttgtgct gttccagata gaactgattc
    24901 accgttttca gccagctgcg cgcgatttca tcacccagaa ggtcatcgcc atacatttta
    24961 aatccctgaa ttgccatcca ttgtaacggt gcccagccat tgggtttatc ccactgttca
    25021 ccggtttcgt actcgcttgc cagaatcccg ccaggtgtca gtaaccggct gcgtacggcg
    25081 tttgccagac gatcggcctg ttcatggttc gccatgccga catagagcgg cacaatggcg
    25141 gcagcggaaa acagcgccag ttgttcgcgt cgccagtcgt aatcgcggta gatgccgttt
    25201 tcatcatccc agaggtaacg gtttaccgca tcgcgacggg cactggcctt ctggcggaac
    25261 agcgcttctg tctctttctc gcctttcagc gccgagatgt tggcgatggc gctctccagt
    25321 ttaaacagga aggcattcag atcgatgggg atgaactggg tggtacgaat gctcgccaga
    25381 cgaccagtat cacgcagcca acgggaagag taatcccaac ctgaggccgc tcccgcgcgt
    25441 aaatcgcggt acacctcgtt gggcgggcga ccagaatgtt tcgcggtttc aacgtcctca
    25501 agccaggatt cgtcacgcgg cgtgtcgcga tcgtcccaat aacggttgag cagcgatccg
    25561 tccggcatcc gcacaacatg gcgataggcc tgattaggga tcaacgattc tgcaccgtcc
    25621 atccagaagg catattccat tttaaggtgg tcgagatagc ggcgcgcacc gcgtacacca
    25681 tcttcttcaa acaactccac catcagcgca aaaaccggtg gttgcgatcg gctcaaataa
    25741 taggtgcggt tgccgttggg gatgtgacca tagttttcga tcatccaggc gaagttatcg
    25801 gccatgcatt tcagcaaatc ttcccgacca ctttccgcca gccccagcat ggtgaaatag
    25861 gaatcccaat agtaggtttc gctaaaacgg ccgcccggga caatatatga ctgcggtagc
    25921 gccagcagag aagaccacgg aatgtgatcc tgtggttcgc gggttagcac cggccacagc
    25981 tggtcgatat gctctttcag ggaattttgc gggtccgata catactcgct ggagtagacc
    26041 tccggcagcc agaagtgatt ttcaacaaac ttgcgcaagt caaaatcacg atgacggcgc
    26101 actttacggt agcggattaa aatatccagc gggtccattt tgggtgcgca gtcgggaaag
    26161 gttttactgt cggggaaaat tttcgccgac tgaacatgct cgaacagttc gagatagcga
    26221 tcggcaggcg tcagcgcatc agaggcgggc agcccttcaa tcatctcggg ttccggttct
    26281 gcctcgatca tctcatccag ttttaattca tacgggtcca gttcatagca gagatcgacc
    26341 tcgatcatca gttcgtctgg attagggttt tgaattttct gattgagcat aaggatccaa
    26401 cctccgaaat tcgtcgctaa ttatcgggct tttctttaag ggtagtcatt cgctccgcct
    26461 ggtgggcgac aaagcgtgcg gtggatcacg tttattaact tgtcaaaaaa tataacgcac
    26521 taatatcaat ggaatttata aaaatacgga tttcgtgttg tttttcattt tcagaacatt
    26581 caatcctgct ctcgccgcaa tcatgatatg ccattcctga tctgggtaat aggcacaggc
    26641 tatcttattg atagtttata ttcatgtaat tgattgattc ctatcaaaaa gcgtatctcc
    26701 ctgtttttta tgatggcttt gtctcgcaga gacgtttgca gcattctgga aaattttctg
    26761 ggtcacaata actcaccacc gtgagggaag aataatgaaa atggtctcac gtattaccgc
    26821 gatcggcctg gctggcgtcg cgatttgcta tttagggtta tctggttatg tgtggtacca
    26881 cgataataaa cgcagtaaac aggccgatgt tcaggcatct gctgtcagtg aaaataataa
    26941 ggttttaggc tttctccgcg aaaaaggatg cgactattgc cacacgcctt cggcagaatt
    27001 acccgcctat tattatattc ctggcgcgaa acagttgatg gattacgaca ttaagcttgg
    27061 atataaatct tttaacctcg aggccgtgcg tgcggcactg ctggctgata aacccgtttc
    27121 gcaaagcgat ctgaataaga ttgaatgggt gatgcagtat gaaactatgc caccaacgcg
    27181 ttataccgcg ctacactggg cgggtaaggt gagtgatgaa gagcgggcgg aaatactggc
    27241 ctggattgca aaacagcgcg cggaatatta cgccagcaat gatactgctc cggagcatcg
    27301 caatgaaccg gtgcagccca tcccgcaaaa actgcctacc gatgcgcaaa aagtggcgtt
    27361 gggctttgcg ctgtatcacg atccccgttt atcggctgat agcaccattt catgcgctca
    27421 ttgccatgcg ttgaatgcgg ggggcgtcga tggcagaaaa acatcgattg gtgttggtgg
    27481 cgcagttggg ccgattaacg cgccgacggt atttaactca gtatttaacg ttgagcagtt
    27541 ctgggatggt cgtgcggcaa cattgcagga tcaggcaggt ggaccgccgt tgaacccgat
    27601 tgaaatggcg tcgaaatcct gggacgaaat tattgctaag ctggaaaaag atccgcagct
    27661 taaagcgcag ttcctcgaag tttatccgca aggtttcagt ggcgaaaata ttactgatgc
    27721 cattgctgaa tttgagaaaa cattaattac gccggattcc ccatttgata aatggttgcg
    27781 tggagatgaa aatgcgctga cggcgcaaca gaaaaaaggc tatcaattat ttaaagataa
    27841 taaatgtgca acttgtcatg gtggtattat tctcggcgga cgttcctttg aaccgttggg
    27901 gctgaaaaaa gactttaact ttggggaaat tacggcggcg gatattggtc gtatgaatgt
    27961 gactaaagaa gagcgtgata aattgcgtca gaaagtaccc ggtttacgta acgttgcttt
    28021 aacggcaccg tacttccatc gcggtgacgt gccgacgctg gacggggcgg tgaaactgat
    28081 gctgcgctat caggtaggca aagagctgcc gcaggaggat gtggatgata tcgtagcttt
    28141 cctgcacagt ctgaacgggg tgtacacgcc gtatatgcag gataaacaat aattaatttg
    28201 atcgcccgaa caggaatgtt tgggcgattt ttattacgat aataaagtct gtttttaata
    28261 ttatc
//
LOCUS       DAZGJW010000058        27866 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZGJW010000058 DAZGJW010000000
VERSION     DAZGJW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.058"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..158)
                     /locus_tag="ACHKIK_002433"
     CDS             complement(<1..158)
                     /locus_tag="ACHKIK_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO9083592.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMII"
     gene            complement(285..1016)
                     /gene="rlmB"
                     /locus_tag="ACHKIK_002434"
     CDS             complement(285..1016)
                     /gene="rlmB"
                     /locus_tag="ACHKIK_002434"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO9083593.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1196..3637)
                     /gene="rnr"
                     /locus_tag="ACHKIK_002435"
     CDS             complement(1196..3637)
                     /gene="rnr"
                     /locus_tag="ACHKIK_002435"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO9083594.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRSGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3676..4101)
                     /gene="nsrR"
                     /locus_tag="ACHKIK_002436"
     CDS             complement(3676..4101)
                     /gene="nsrR"
                     /locus_tag="ACHKIK_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO9083595.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4306..5604)
                     /gene="purA"
                     /locus_tag="ACHKIK_002437"
     CDS             complement(4306..5604)
                     /gene="purA"
                     /locus_tag="ACHKIK_002437"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO9083596.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5708..5905)
                     /gene="yjeT"
                     /locus_tag="ACHKIK_002438"
     CDS             complement(5708..5905)
                     /gene="yjeT"
                     /locus_tag="ACHKIK_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO9083597.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5987..6991)
                     /gene="hflC"
                     /locus_tag="ACHKIK_002439"
     CDS             complement(5987..6991)
                     /gene="hflC"
                     /locus_tag="ACHKIK_002439"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO9083598.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6994..8253)
                     /gene="hflK"
                     /locus_tag="ACHKIK_002440"
     CDS             complement(6994..8253)
                     /gene="hflK"
                     /locus_tag="ACHKIK_002440"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO9083599.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8339..9619)
                     /gene="hflX"
                     /locus_tag="ACHKIK_002441"
     CDS             complement(8339..9619)
                     /gene="hflX"
                     /locus_tag="ACHKIK_002441"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO9083600.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTGRLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9696..10004)
                     /gene="hfq"
                     /locus_tag="ACHKIK_002442"
     CDS             complement(9696..10004)
                     /gene="hfq"
                     /locus_tag="ACHKIK_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO9083601.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10090..11040)
                     /gene="miaA"
                     /locus_tag="ACHKIK_002443"
     CDS             complement(10090..11040)
                     /gene="miaA"
                     /locus_tag="ACHKIK_002443"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO9083602.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAVEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(11033..12880)
                     /gene="mutL"
                     /locus_tag="ACHKIK_002444"
     CDS             complement(11033..12880)
                     /gene="mutL"
                     /locus_tag="ACHKIK_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO9083603.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12890..14227)
                     /gene="amiB"
                     /locus_tag="ACHKIK_002445"
     CDS             complement(12890..14227)
                     /gene="amiB"
                     /locus_tag="ACHKIK_002445"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO9083604.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14246..14707)
                     /gene="tsaE"
                     /locus_tag="ACHKIK_002446"
     CDS             complement(14246..14707)
                     /gene="tsaE"
                     /locus_tag="ACHKIK_002446"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO9083605.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14679..16226)
                     /gene="nnr"
                     /locus_tag="ACHKIK_002447"
     CDS             complement(14679..16226)
                     /gene="nnr"
                     /locus_tag="ACHKIK_002447"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO9083606.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16225..17364
                     /gene="queG"
                     /locus_tag="ACHKIK_002448"
     CDS             16225..17364
                     /gene="queG"
                     /locus_tag="ACHKIK_002448"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO9083607.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAAKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17347..17400)
                     /locus_tag="ACHKIK_002449"
     CDS             complement(17347..17400)
                     /locus_tag="ACHKIK_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083608.1"
                     /translation="MRFYFYSQAVDESGVTW"
     gene            complement(17634..17709)
                     /locus_tag="ACHKIK_002450"
     tRNA            complement(17634..17709)
                     /locus_tag="ACHKIK_002450"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17674..17676),aa:Gly,seq:gcc)
     gene            complement(17745..17820)
                     /locus_tag="ACHKIK_002451"
     tRNA            complement(17745..17820)
                     /locus_tag="ACHKIK_002451"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17785..17787),aa:Gly,seq:gcc)
     gene            complement(17978..18053)
                     /locus_tag="ACHKIK_002452"
     tRNA            complement(17978..18053)
                     /locus_tag="ACHKIK_002452"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18018..18020),aa:Gly,seq:gcc)
     gene            complement(18264..18809)
                     /gene="orn"
                     /locus_tag="ACHKIK_002453"
     CDS             complement(18264..18809)
                     /gene="orn"
                     /locus_tag="ACHKIK_002453"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO9083609.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18904..19956
                     /gene="rsgA"
                     /locus_tag="ACHKIK_002454"
     CDS             18904..19956
                     /gene="rsgA"
                     /locus_tag="ACHKIK_002454"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO9083610.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            20053..21021
                     /gene="asd"
                     /locus_tag="ACHKIK_002455"
     CDS             20053..21021
                     /gene="asd"
                     /locus_tag="ACHKIK_002455"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO9083611.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            21043..24366
                     /gene="mscM"
                     /locus_tag="ACHKIK_002456"
     CDS             21043..24366
                     /gene="mscM"
                     /locus_tag="ACHKIK_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO9083612.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24395..24709)
                     /gene="yjeO"
                     /locus_tag="ACHKIK_002457"
     CDS             complement(24395..24709)
                     /gene="yjeO"
                     /locus_tag="ACHKIK_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO9083613.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(24706..25020)
                     /gene="yjeN"
                     /locus_tag="ACHKIK_002458"
     CDS             complement(24706..25020)
                     /gene="yjeN"
                     /locus_tag="ACHKIK_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO9083614.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            complement(25072..26574)
                     /gene="yjeM"
                     /locus_tag="ACHKIK_002459"
     CDS             complement(25072..26574)
                     /gene="yjeM"
                     /locus_tag="ACHKIK_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO9083615.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(26793..27770)
                     /gene="epmA"
                     /locus_tag="ACHKIK_002460"
     CDS             complement(26793..27770)
                     /gene="epmA"
                     /locus_tag="ACHKIK_002460"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO9083616.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
BASE COUNT         6545 a         7787 c         7003 g         6531 t
ORIGIN      
        1 tcgataatca tttggcggga agtaaaaaga atattgattt gcaaatcgcc ataatcattc
       61 attttaataa ttaatgcgct ctcgctattt gtcacatcaa tattttgttc aggtacagtt
      121 tgcagcgccg tcgccagcgc caacggattc catgtcatat attattccat atagatttag
      181 tttaaatatt aataaaatga atatttgcag tacgtaatta tcttaccagc tatagacaaa
      241 aaaaaaccat ccaaatctgg atggcttttc ataattcaga cgaactagct gcgctgacga
      301 accgcttcaa ataagcaaat tccggttgca accgaaacgt tcagggaaga aacacttcct
      361 gccattggga tgctgatcaa ctcatcgcaa tgttcacggg tcaggcgacg cataccttca
      421 ccttccgcgc ccatcaccag cgccaggcgt ccggtcattt tgctctgata gagtgtatga
      481 tccgcctcgc ctgccgtacc gacgatccag atattctctt cctgcaacat acgcatggtg
      541 cgcgcaaggt tagtcacccg aatcagtgga acgctttctg ccgcgccgca ggctactttt
      601 ttcgccgtag cgttgagctg tgcggagcga tctttcggca caatcaccgc gtgaacgcca
      661 gcagcgtccg cgctacgcag gcacgcgccg aggttgtgcg gatcggttac accgtcgagg
      721 atcagcagga acggttgatc gagcgaagcg atcagatccg gcagatcgtt ttcctgatac
      781 tgacgtcctg gcttcacgcg ggcgataatg ccctgatgca cggcaccgtc gcttttctcg
      841 tcgagatatt ggcggtttgc caactggata accacgccct gagactcaag ggcgtgaatc
      901 agcggtaaca gacgtttatc ttcacggcct tttaaaataa agacttcctg aaaacgttca
      961 ggggcgcgct ccagcagggc ctgcactgcg tggatgccgt aaatcatttc gctcattaat
     1021 gtactcgttg tttacgtttt gcctgatgca ctacgtttat ccggcctaca tgatctctgc
     1081 aatatattga atttgcgtga ttatgtaggc cggataaggc gttcacgccg catccggcat
     1141 gaacaaagcg cattttgtca gcaatctaac cctcttcttt taaagagggt attgatcact
     1201 ctgccacttt tttcttcgcc gcacgcttcg ctttggtcgc tgcggctatt ttctgcgttt
     1261 tcgccgatgg ctttttcgct tttctcgcgt ctttcttcgc cgctttcggc ttcgtttttt
     1321 tctcaccgcg gaaggcgctg tctggctcaa agtttacctt tttaccgacc tgacgacgct
     1381 tgccgccttt tttgcctgca tcgccttttt tcgctttctc gcgcgccgtt ttaccgacgt
     1441 tgcgcggtgc gcgttcactg gagatcagac taaagtcgat tttgcgctcg tccatattaa
     1501 ccgcttcgac tcgaacttcc acgcgatcgc ccaggcgata agtctggccg ctggattccc
     1561 ccatcaggcg ttgccctacc tggtcaaagc gatagtagtc attgtccagc gaagagacat
     1621 ggaccagacc atcaatgaac aagtcgtcca gacggacgaa gaagccaaag ccagtgacgc
     1681 tggaaattac gcctttaaag acgttaccta cctggtcgag catgaagtca cacttcagcc
     1741 agtcagcgac atcgcgcgtt gcttcgtcgg cacgacgttc cgccatcgaa cagtgctgac
     1801 ccagttgcaa catctcttcc atcgaataat ggtagccgcc ggtttcagtg gtgttgccct
     1861 gatgcccctg ctctttcgcc agcagatatt taatggcgcg gtgcagcgtc aggtcaggat
     1921 aacgacgaat cggcgaagta aagtgcgcat aggactgcaa tgccagaccg aagtgaccac
     1981 ggttttctgg atcgtaaatc gcctgtttca tcgagcgtag cagcatggtt tgcagcattt
     2041 ctgcatcagg acggtcggca accgactcca gcagttccgc gtagtcacgc ggttccggct
     2101 tgttaccacc cggcagctcc agccccagct ccgccagcac tgaacggaaa gaggtaatcg
     2161 cttcggtgct cggcttgtcg tgaatacgga acagtgccgg ttctttcgct ttctcaacga
     2221 aacgcgccgc cgagatattc gccagaatca tgcactcttc aattaatttg tgcgcgtcgt
     2281 tacgctgggt ctgttcgata cgttcaatac ggcgttcagc gttgaaaatg aacttcgctt
     2341 cttcgctctc aaatgagatc ccaccgcgtt cttcacgggc tttatccagc actttataga
     2401 ggttatgcaa ctcttcgaga tgcttaacca gcggggcgta ctgctcgcgc agatcctgat
     2461 cgccctgcag aatatgccag actttggtgt aggtcagacg cgcgtgagag ctcatcaccg
     2521 cttcgtagaa tttgtagccc gtcaggcggc ctttcgacga aaccgtcatc tcgcacacca
     2581 tacacaggcg gtctacctgc gggttgagcg aacacaggcc gttagagagc acttccggca
     2641 gcatcgggat aacctgcgaa gggaagtaca ccgacgtgcc acggttacgc gcttctctgt
     2701 ccagcggcgt tggcggacgc acatagtagc tgacgtcggc aatcgcgacc cataaacgcc
     2761 agccgccgct gcgttttttc tcgcagtaaa ctgcatcgtc aaagtcacgg gcgtcttcgc
     2821 catcaatggt gaccagcggt aaatcgcgca gatcaacacg gcccgctttt gcttcttccg
     2881 gcacttcttc tttcagcccg gcaacctgtt gctcaacagc ctgcggccag atgtacggaa
     2941 tttcatgggt acgcagagcg atatcaaccg ccatgccggt gcccatattg tcgcccagca
     3001 cttcgacgat tttacccacc gctttggtgc ggcgagtcgg acgctgagtc agttcgacta
     3061 cgaccacaaa gcccatccgc gcgcccatga tctgatcggg cgggattaag atatcgaagc
     3121 tcagacggct atcgtcagga accacaaagc cgacgcccgc ttcagtaaag tagcgaccaa
     3181 caatctggct ggtttttggc accagtacgc ggacaatacg cgcttcacga cgacctttac
     3241 ggtcagcgcc cagcggctgc gccagcacct gatcgccatg aatgcaggtt ttcatctgct
     3301 cgctggagag atacaaatca tctttacgcc cttcaacccg cagaaagccg tagccatcac
     3361 ggtggccaat aacggtacct ttcaccaggt cgaggcgttc cggcagcgca tagcactgac
     3421 gacgagtgaa gaccagttga ccatcgcgct ccatcgcgcg caggcggcga cgcaggcctt
     3481 caagctgctc ttcgccttca atgtgcagtt ctaccgccag ctcatcacgg ctggccggtt
     3541 tttcacgttt ggttaaatgt tcgaggataa attcccgact agggatggga ttcgcgtatt
     3601 tttcagcttc gcgttcctgg aaaggatctt gtgacatctc ggttcctccg ttgtcatctc
     3661 tgatgaagat tttcgtcact ccaccagcaa taatttataa agcggttgat tctcttcaac
     3721 caaatcggca agcgtgtagt tatccagttc cgtaagaaaa ctttgcacgg ccttagaaag
     3781 cgcctgtttc aaccgacagg caggtgtaat gtggcaaaac tcactgctgc aattcaccag
     3841 cgataagggc tccagctcgc gcaccacatc accaatacgt atcgcactcg ccggtttacc
     3901 caggcgaatg ccgccatttt ttccacgaac agcagtcacg tagccggcac gactaagttg
     3961 attgattatt ttgaccatat gattacggga gacgccgtag acgtcagtca cttcagaaat
     4021 actggtcatc cgcccttctg gcaatgacgc catgtagatc agcgcacgta atccgtaatc
     4081 agtgaaactc gttaactgca catcaacctc aaaagggaaa tcgggaaaaa atacatttat
     4141 attgatgata aaccagccac aagctgtgtc gctaatttat ttcagttcgg ggaaggaaaa
     4201 aagcgaggat ttaacactgt gtcggatagc gtaatggctt aaccgacaca gcaatgtatc
     4261 acactgttag cccggcaggc aaaatatctg ccaggcgtac cagaattacg cgtcgaacgg
     4321 atcgcgcaga atcatggttt cagtacgatc cggaccggta gagatgatat cgatcggcac
     4381 accagtcagc tcttcaatac gcttgatgta gttcagcgcc gcctgcggca gaccgctacg
     4441 atctttcacg ccgaaggtgg attcagacca gcccggcatg gtttcgtaaa tcggctctac
     4501 acctttccag tcgtcagctg ccagcggagt ggtcgtcact tcacgaccat ccggcatacg
     4561 gtaagccaca cagagtttca cctctttcag gccatccaga acgtccagtt tagtcaggca
     4621 gaagccagac agggagttca gctgtaccgc acgacgaacg gcaacggtgt ccagccagcc
     4681 ggtacgacga cgacgccccg tagttgcgcc gaattcgtta ccctgcttgc agaggaactc
     4741 gccagtttca tcaaacagtt cggtcgggaa cggacctgca cctacacgag ttgagtaagc
     4801 tttgaggata cccagaacat aatcaacata acgcgggccc aggccggaac cggtcgccac
     4861 gccaccagca gtggtgttgg aagaagttac gtacggataa gtaccgtggt cgatatccag
     4921 cagcgtaccc tgcgcacctt caaacatgac gaaatcgcca cgctgacgcg cctggtcgag
     4981 caggtcagaa acgtcaacca ccatagaagt caggatgtcg gcaacagcca tcgtatcatc
     5041 cagaactttc tggtaatcaa ccgcttcagc tttgtagtag ttaaccaact ggaagttgtg
     5101 atattccatc acttctttca gtttttcagc gaaggtttct ttgtcgaaaa ggtcgccaac
     5161 acgcagaccg cgacgtgcta ctttatcttc ataagcaggc ccgataccac gaccggtggt
     5221 gccgatcgct ttcgcgccac gcgctttctc acgcgcgtta tccagcgcaa cgtgataatc
     5281 aaggatcagc ggacatgctt cagacagcag cagacgctca cgaacgggga tgccacggtc
     5341 ttccagttct ttcatctctt tcatcagcgc ggccggagac agcacaacac cgttaccgat
     5401 gatgctggtt acattctcgc ggagaatacc tgatggaata agatggagaa cggttttttc
     5461 accgttgatt acgagagtat ggcctgcgtt gtgaccgccc tggtagcgta caacatattt
     5521 agcccgttca gtcagaagat cgacgatctt acctttacct tcgtcacccc attgggtgcc
     5581 cagtacgacg acgttgttac ccatttttca aaatcaccgt ttgcttaaaa atggattcta
     5641 ccatcgcttt ttcagagtta cagcactttt tgcactcaaa aatgacctcg atcagtctgt
     5701 tttttgttca gccaatcgtt ttcctcaaca tgtagtagac cacaacgccc gcaaccacaa
     5761 gtccaccgcc aaaacgacgt aaaatattat cgggcaaatt ggtcatcgca gagatcatct
     5821 tcttccatgc cttcgggtaa agcatcggcc ctaaaccttc cagtaccaaa accagggcaa
     5881 gcgccagcca gattgtcgaa ttcattcatt atccttatag aaaaagaaaa ccaccgacat
     5941 ccctgaggat gcggtggctt tattgacctg taccgcagtc gttatattaa cgcgttgcgg
     6001 aagtcggcgt cttcatgtag cggaagaaat cgctatccgg gctcatgacc atcacgtcct
     6061 gattgccaga gaagctgttc tcataagcac gcaggctacg gatgaatgcg tagaagtccg
     6121 gatctttact gaatgcatca gcaaacagtt tggctgcttc ggcatcgcct tcaccacgca
     6181 tgatgcggcc ctgacgctca gcttctgcca gcgttctggt cacttcatag tcggcagtcg
     6241 cgcgcagttt ttccgcttct tcctgacctt gtgaacggtg acgacgcgct accgcttcac
     6301 gctcggcgcg catacggttg tagatcgctt cagacacttc ggtcggcagg ttgatctgct
     6361 tgatacgcac atcgacaact tcaataccca gcgccgccat actgttcggg ttgatgaccg
     6421 gaactttgcc cttcgtctca gccgttacgc gctctgccgc ttcggcaatg gcgttatctg
     6481 ctgccggggt agtaacttca tcttctgtac ccgcagaacc ggagttcagc gcgtcacgta
     6541 cttcgagggt cagacgacca cgggaatcag tgacgatatc tttcacgtcc aggcgaccaa
     6601 tttcagaacg cagacggtca gagaacttac gtttcaacag cacttccgct tgcgaaatgt
     6661 cgccaccacc cgttgccagg tagtaacggc tgaaatcgct gatgcgccat ttgatgtaag
     6721 aatcgacgat caggtctttc ttctctttgg tcacaaagcg gtcggcctgg ttgtccatgg
     6781 tctgaatacg tgcgtcgagc attttcaccg tttcaatgaa cggtatcttg aaatgcagac
     6841 ccggctcata aaccagaggt ttgttgtcat cgtcacgcag taccttacca aaacgcagcg
     6901 taataccgcg ctcaccttct ttgacgacaa agacagacat gtaaagcact accagcacga
     6961 tgatgataat cgcgataact gacttacgca tcgttattcc ccctgacgct ggtagtcgtt
     7021 acgctgcgcg ttggcgcggc gttggtccat aatatcgccc tgactggtgg acgacgtgtt
     7081 gcttgctcca ctggttgtgg aggaagaggc tggcggcaga cgcagcagat tgctggcacc
     7141 gttatcgctc ttcgccgcag gggcgttacc acctttcagc atctggtcta acggcagaac
     7201 catcaggttt ccacctttgt cgttaaccag cactttgcgg gtgttgccca acactttttc
     7261 catcgtctcg atatacagac gctcgcgagt aatttccggc gcggctttat attccggcag
     7321 aagtttagca aagcgcgcca cttcaccctg agcttccagg atggtctggg ccttgtacgc
     7381 acgcgcctct tcgaggatac gttgcgcctg accgttcgca cgcggctgaa cttcgttggt
     7441 atacgcttct gcttcacgaa tgtattgctg ttcgttttca cgcgcggcaa tcgcatcgtc
     7501 aaacgccgct tttacttctt ccggcggacg agcagcctgg aagttgacgt ccagcagcgt
     7561 gatacccatg tcatacggac gaatcgtctc ttccagttcg cgctgagtat cgctacgaat
     7621 cacggtacga ccttccgtca gaatgcggtc catggtgtat ttaccgataa ctccacgcag
     7681 ggcgctgtcg gtagcctgac gcaggctgtc atccgggctg gtcacgctat acagatattt
     7741 ttccggattg gtgacgcggt actgcacgtt catctcaacg cgcactacgt tctcgtccga
     7801 cgtcagcatc acaccagaag cggccagttc acgcacggct tccacgttca ccggtttgac
     7861 ttcgtcgata aacgtcggtt tccagttcag acccggctca accagatggc tgaatttacc
     7921 aaagcgtgtt accacgccgc gttcggcttc tttaatggta tagaaaccac tggccgccca
     7981 gataatgaca atcgctgccg ctgcgatggt aacgacacga ccgccaagct gcgggcgcgg
     8041 gccttgcgat gaactgccac cgccagatcc ggtgccttta ccgccgccca gaccaccgag
     8101 ctttttgctc agtttgcgga agatatcatc taaatcaggt ggcccttgat cgcgaccgcc
     8161 tttgtttcca tttccctcag agttgccgcc aggtttgctg cttccccacg ggtcgcggtc
     8221 ttgtccgtta ttaccgggct gattccacgc catgtttgtg ctccatattt gttatgcggt
     8281 gatccctgtt gtctttcgac gccagaggat atgactccac gcttcagacg ctacgccgtt
     8341 agatcaggta atcgatcaac gccggttctt gtttacagag gcgacgccag tcaacgatcg
     8401 gcatacgaac ttgcagactt acgctgccgt cctcctccat ccactctttt tctattgcct
     8461 gaagctgata aaaacgactt ctcagacgcc cttcctgcgg tggcagacgc aatgtatgct
     8521 gcgccacctc gccggaaagc cgccccgtca aagcctgaaa aagctgtggt atccccgctc
     8581 cggtctgtgc ggaaagccag acacggatcg gtttgttctc ttcgtcccga tcaatacgcg
     8641 gttcgaaatc ttccagcata tcgatcttgt tcatcaccag cagggttggg atctcgtgag
     8701 cgtcgatctc ttcaagaacc gtattcaccg cttcgatgtt ttcttgtaca cgcacatccg
     8761 ccgcatcaat gacgtgcagc agtaatgtgg cttgccgcgt ctcttgtaac gtggctttaa
     8821 atgccgccac cagatcgtgc ggcaggtggc gaataaaccc tacggtatct gcaagtacgg
     8881 tttcaccgac atctgcaacg tcaatacgcc gcaacgtcgg gtcgagggtg gcaaacaact
     8941 ggtctgccgc gtagacccgc gcttcggtga tgcgattgaa aagggtagat ttaccggcgt
     9001 tggtatatcc caccagcgaa acagtaggaa cgtcggcttt gatacgcgat tgccgcccct
     9061 gctcacgctg cttttcaact ctttccaggc gtgactgtat ctgcacgatg cgattacgca
     9121 acaaacgacg gtcggtttcg agctgggttt cacccggacc acgcaaacct atcccgcctt
     9181 tctgtctttc aaggtgggtc cagccacgca ccaggcgcgt agccagatgg cgcagctgcg
     9241 ccagctcaac ctgcaactta ccctcatggg tacgcgcacg ttgggcgaaa atatctaaaa
     9301 taaggccggt gcggtcgata acacgacact cgcacaaacg ctccaggtta cgctcttgcg
     9361 ccgggctcag ggcatggtca aaaagaacga ccgaagcacc cgtcgctttg acagcttccg
     9421 caatttcaac tgctttacct tcacctacaa aatactttgg gtgcggcgct ttacggctac
     9481 cggtaatcac ctgcaatgct tcgacaccgg cggaagagac cagagattca aactcctgga
     9541 ggtcttccat atctttgtct tgcgtaaaat agatgtgtac cagtaccgcc tgctcaccag
     9601 catcataacg gtcaaacaag cgtataaccc tctaaataga tcagcgggga acgcaggatc
     9661 gctggctccc cgtgtaaaaa aaacagcccg aaaccttatt cggtttcttc gctgtcctgt
     9721 tgcgcggaag tattctgcgc gctgctacca tgatggtagt tactgctggt accgccaccg
     9781 gcgttgttac tgtgatgaga aaccgggcga gacgggacaa cagtagaaat cgcgtgcttg
     9841 taaaccatct ggctgaccgt gtttttcaac aggatcacga attgatcaaa agactcgatt
     9901 tgcccttgca gcttaatacc attcaccaaa taaatagaaa ctggaacacg ttcccgacgc
     9961 agtgcgttca ggaacggatc ttgtaaagat tgccccttag ccattctctc ttttccttat
    10021 atgcttattt gtactttgaa cctttcgatt ctgaaaaaat tgcgcacgat acgtctcaat
    10081 tgtacacatt cagcctgcga tagcaccaac aacctgtaat acttcgtcgc gcgcctgttc
    10141 tggtttttca ctgtcaagcc agtgaacccc ttcccaacca cgcagccagg ttatctgccg
    10201 cttcgccaac tgtctcgtgg cgcaaacacc tcgataaacc atttcatcgt atgagatttc
    10261 gccttcaagg taagaccaca tctggcgata acccacgcaa cgaatggaag gcaagtccgt
    10321 atgcaaatct cctcgggcaa aaagcgcccg gacttctgct tcaaaacctg aagccaacat
    10381 ctgatgaaaa cgctgctcaa tgcgttgatg gagcagttca cggctcgccg gggcgatggc
    10441 gaactgatgc acctgatacg gtagagcgtc tcctgacgtt tgcgtcagtt ccgttaaagt
    10501 tttacccgaa atgaaaaaaa cttccagtgc ccgggaaagc ctttgtggat catttggatg
    10561 aatccttgct gccgcaaccg gatctacctc ctgaagttga cgatgcaatg actcccaacc
    10621 ttgctctacc gcctgttgct caattctggc ccgcacttcc gggtctgccg acggtagcgg
    10681 cgacaaccct tccagcaatg ccttgaaata caacatcgta ccgcccacta acagtgggat
    10741 ccgccccgcc gcggtgatat cggccatttc cgccagcgca tcgcggcgaa aatcagcagc
    10801 cgagtaagcc tgcgacggat cgcgaatatc cagcaatcgg tgcggcgcgg cgagtaactc
    10861 ttcagcgttc ggcttcgccg tcccgatatc catccctttg taaataaggg cagaatcaac
    10921 gcttatcaac tctactggta aaattttacg cagctcaatg gctaacgccg ttttaccgga
    10981 ggccgtcggc cccatcaaaa aaatcgcctt aggcaggctc gccttactga tatcactcat
    11041 ctttcagggc ttttatcgcc ggatgtaaat caacagattg taacagacca cccggcggcg
    11101 ttttcacaag ttgcggacat aaccgttcca cgtccgccag cagggttatg gcctgtgcca
    11161 ttgaccactg cgcatgttcg ctcatcagat ttcgtgcaat ccactgcgca atattgccag
    11221 gttcgaatac ggactgcttc gccaggtagc ctatcagttc aggaatcaag atttgtaaat
    11281 tttgttggcg taagggtaaa ggcacggccc tgatggtcac atgctgtgca tctgactgga
    11341 aatcaatacc caattccgcc agggcagact gcgctttttc taatgccgat ttttcttcgc
    11401 cagaaacttt tagccgcaac ggaatcagca acggctgggc gcaaactggc gcttcacccg
    11461 gcgtcagttg cgcctgacgc agccaacgtt ctgccactgg caaggctaaa agtgaaatgt
    11521 tgccatcgcg ctccagcaac gcacagtcgg aatggacgat agtcagtacc cgaccaaaac
    11581 tctgactgtt cgccgcaagt gcaggttcct gcggttccgg cgcttttggt ttttgcatcg
    11641 gcgcgggcgt ttgcaaaagc tggcgataca cttcaccttg ctgtttctgg tagcctggct
    11701 gcgcattcgg ccagggggca gccggacgac tgcctgaggc gggcgcagga gagtagcgcg
    11761 gagctaccgg ctcacgagct gccggttctg caaagtgatt gcgccccgcc gccacgcggt
    11821 tttccggaat gggacgcggt gcaggttggg gttcatcgtc cagcggtagc ggcgtttcca
    11881 gttgctgttg tagcacgctc agcacgccct gatagataaa atcatgcacc agacgcgact
    11941 gatggaaacg cacttcgtgt ttggcggggt gtacgttgac gtccacctga tgcgggtcga
    12001 tctccagata caacacaaac gccggttgtt gatcggcccc cagtttgtct tcgcaggcct
    12061 ggcggatcgc atgattgatc aggcgatcgc gcatcatgcg accattcacg tagcaatact
    12121 gaatttccgc cagtgcgggc gtagtgtgat ttggatcggc cacccagccg cgcagcgtga
    12181 gatcgccgtg ttgccattca atcgccagcg cttgttcgag aaaagcggtg ccacaaatcg
    12241 cgcctaagcg ccgttctttt tgcccgcctt ccggcactgc gcggtactga cgcacaattt
    12301 taccgttatg cgacaggttg atcgtgacgt cgaaacgcgc cagcgcaatg cggcggatga
    12361 tttcatcaat atggctaaat tcggttttct cggtgcgcag gaatttgcgc cgcgccgggg
    12421 tgttgtagaa cagatccagc acctccagcg tcgtccccac aggatgcgcc gccggtttta
    12481 ccgtcacgtt catatcgcgc ccttcggcat aggcctgcca ggcttcctgc tgttctgcgg
    12541 tgcgtgaagt gagcgtcagg cgggaaaccg aactgatact cgccagcgcc tcaccgcgaa
    12601 agcccaggct gataatggct tcgagatcgt ccagagaggc gattttactg gtggcatgac
    12661 gagccagcgc cagcgccagc tcatcttttt tgataccgca gccgttatca cgaatgcgga
    12721 taagtttcgc cccaccgcgt tcgatatcaa tatcgatacg cgtcgcacct gcatcgaggc
    12781 tgttttccac cagttctttg actaccgacg caggtcgctc gaccacctca cctgcggcaa
    12841 tctggttcgc cagttgtggc ggtaagacct gaattggcat caatcgtcct tagtttggca
    12901 gcgtgcgatc tggcgtcgtc accgtactgg cagtttgtgc cgttgccccc tgcggcgcag
    12961 attgcatcgg atgcgcaagg aaataattgc gcaggccttt gtaaatggct tctgccagct
    13021 gttgttggta atcgtcgctc gccagcaaac gttcttcgct gttgttgctg ataaaaccgg
    13081 tttcgaccag tactgacggg atatccggcg aacgcagaac gccgagactc gcgtgttctg
    13141 gtcggcgttt atgtatttcg ccaatgcgtt gcaactgact gatcatactg gtcgctacat
    13201 cataccctac ccgttgggaa tgaccgaact gtaaatccag caccgcctgg cttaaatagg
    13261 ggtcagactg actgttcgcc agcacatcac ccgccccacc cagcagctcc gactgtttct
    13321 cgtgctgctc cagccagctg gccatttcgc tgttggcgcg acggttagag agcacccata
    13381 cggaagcgcc agtcgcactg cggttcggtg cggcatcagc gtgaatcgac acgaggaaat
    13441 tggcgttttg cttacgtgcc acatcactgc gccccatcac cgagataaag taatccccgt
    13501 cacgggttaa aacgccctta aacatcggat cgtcattgag caaagtacgc aatttacgcg
    13561 cgatggcgat ggtgacattt ttctcccgcg taccaccggg gccgatagcg ccagggtcct
    13621 gaccgccgtg tccggcatca atagcgatga taattttatc gccagtgtta gccgtcgcgc
    13681 gcgctgccgg acgcgttacc gtattactgc tgataacacc cgtagtgcgg ttactttccg
    13741 ttttaaacgg attgcgcgcc ggttcgctga cgcgcggtgc gacaaccgca ggcgtttcaa
    13801 cgcgtttcgc aaccacgggc ggcggaggag gaggtggcgg cgcatcggcg ttaatcgtaa
    13861 agacgacagt gtaattgctg ccattctgcc gcttcaccgc ttcggtttta ccgttttcgg
    13921 taagatcgac caccagccgt agcgtttgtg catctttagg cgttccagag cgaatcgcct
    13981 tcaccagatt attgccgctg aacaacaacg gcagtccctg aatcacgccc gtttgtttga
    14041 tatcgagcgc cacggtgcgt ttgctttgat ggctaaacgc ataatcagga tcgccaataa
    14101 aactcaacgt tatccgcgcc tgttgattac cgttagaaac ctgaatatca gagagcgtcg
    14161 cggcacccac cggcgtgcac agcagcagca gcgtcgctac caaccaattt ctgatgcgat
    14221 acatcatccc gccacctttc aaaggttaac cggctaaacg cgccagcaac aattcacccg
    14281 cagaggaaac cgcactcacg cgcgcctcac ggccttgtgc ctgataatca atgtgtattt
    14341 cgacatccgg gtcaggaaga acacctgtac cttgttgtgg ccactccacc aggcagatgg
    14401 catcgttggc aaaataatcg cggatcccca taaactccag ctcctcggga tcggcaaggc
    14461 ggtacaaatc aaagtgataa accattaaat tgtcgagcgt atagggttcg accagcgtat
    14521 aagtggggct tttgacatta ccctgatgac ccagagcttg taaaaagccc cggctaaagg
    14581 tggttttacc tgcgcctaaa tcgccataca gatagattac ggttgcgcca tcgcaggctt
    14641 tcgctacccg ctcgcccagg tctaatgttg cctgctcatc agggagcgga attactcgat
    14701 tcatcatggt ttttatcagt cacttccggg ttaacaatac gctgtagcgt ggaaaagaga
    14761 tcggttgcca gcatcccgcg cgttccaaaa cgcgccgcca gtacgtcagc tgcagcaccg
    14821 tgcgcgacac agcccgcaca ggcggcatca tacggcgaca gtttttgccc aagcaatgcg
    14881 ccaataatac cagagagcac atcgcccatg ccgccgctcg ccatgcctgc atttccggca
    14941 tcaataatgc ctaaagcgtc aggatgggcg gcgaccacgg ttccggcacc tttcagcacc
    15001 gctacgccgc cataacgttg taccagacgt ttggcgcaat gtaagcggtc actttcaatt
    15061 tcagcgacgg aacagcctaa caaccgtgcg gcctcgccag gatgcggcgt gatcacgcga
    15121 ttgtgacgct tatcgggatt gattgccagc aggttcaatg catcggcatc ccacaacatc
    15181 ggtttgcgaa aattctcaac tttttgcagt gcttttttcc cccactcttg ctggcccaga
    15241 ccgggaccaa tcaccaccac atcggcccat tccaggcttt cggtaagaga gtccatcgtc
    15301 agttcatgca ccatcaattc cggtcgtgca gtcagcagcg gcgcaatgtt ttcactgcgg
    15361 gtcagtactc ggactaaacc agcaccagca cgtagcgccg cttcccccgt catacgaata
    15421 gcccccgccg tgccgtgatc gccaccgata attaccagcc gcccgtgatc gcctttatgc
    15481 gaagtcgggc gacgcggttt tagccagtga gaaagttgtt ctgccgaaaa ccgctgaatt
    15541 ttcgtctcct gacctgccag ccaactatcc agccccagtg agtcaaaatg cagttgtccg
    15601 gtaacatccc gcgcttttcc agtgagcaag cctggtttca gcgcaataaa agtgatggtg
    15661 tgatcggcgt tgatcactgc gcctggcgta gcgccggttt cagccagcag gccggaaggg
    15721 atatcaaccg ccgcaatcgg cgcaggatgg gaattagcgt ggtcgattaa ctggctaatg
    15781 gattcgcggg gcgcttgctg caagccggta ccgagcagcg catcaacaat cagatctacc
    15841 gattcgggcc agacaatatt cgaagcatgg atctcgcctc ccgcgtttaa ccatgcttcg
    15901 cgtgccagcg cggcctcttc cggcaacggt ttgtcgctct cctgggccaa caacgtgacc
    15961 tcaatgccga ccgctgtggc cagtcgcgcg accacgtagc catcgccgcc gttattacca
    16021 tgaccgcaca gcaccagcca gtggcgggcg tcaggatacg ccgaacgaca cacctggaat
    16081 gcggcttcgc cagcgcgaag catcagctca tagagtgtaa gccccagcgc atctgccgcc
    16141 tcacgttctc cgcggcggat atcgtcggcg tgccagacgg tgtgtggtat acttacgggg
    16201 tttttcttca ttgtatggtc cgtcatgtca gagcccctcg atctcaatca gttagcgcaa
    16261 aaaattaaac agtgggggct ggaactgggc tttcagcagg taggtattac cgataccgat
    16321 ctcagcgagt ccgagcccaa actgcaagca tggctggaca aacaatacca cggcgaaatg
    16381 gactggatgg cacgtcacgg tatgctgcgc gctcgccccc atgagttatt acccggtacg
    16441 ctgcgcgtga tcagcgtgcg gatgaattac cttcctgcta acgccgcatt tgccagcacg
    16501 ctgaaaaacc ccaaactcgg ctatgttagc cgttatgcgc tgggccgtga ctatcacaaa
    16561 cttctgcgca accgactcaa aaagctgggc gagatgattc agcaacattg tgtttcgctg
    16621 aattttagac cgtttgtcga ttctgcgcct attctcgagc gcccgttagc tgcaaaagct
    16681 gggctcggct ggacaggtaa gcactcactt atcctcaatc gcgaggccgg ttctttcttc
    16741 tttttaggcg aattgctggt cgatattccg ctgcccgtgg atcaaccagt cgaggaagga
    16801 tgcggtaaat gcatagcctg tatgacgatt tgcccgaccg gtgccatcgt cgagccatat
    16861 accgtcgatg ctcgccgctg tatctcttat ctcaccatcg aactggaagg ggcgatcccg
    16921 gaagagttgc gaccgttaat gggaaaccgt atttacggtt gcgatgactg ccagcttatc
    16981 tgcccgtgga atcgctattc gcaactcact acagaagacg atttcagccc gcgtaagccg
    17041 ctacacgcac cggaactcat tgagttattc gcctggagcg aagagaagtt tttaaaagtc
    17101 acggaaggat cggcgattcg ccgtataggt cacctgcgct ggctgcgtaa tatcgccgtg
    17161 gcattaggca atgccccctg ggatgaaacg attctggcgg cgctggagag tcgcaaaggt
    17221 gagcacccac ttcttgatga gcacatagcg tgggcgatgg cgcagcaaat cgagaggcga
    17281 aatgcgtgca tagtcgaagt gcaattgccg aaaaaacagc gtctggttcg ggtgattgaa
    17341 aaagggttac cacgtgacgc ctgattcatc cacagcttgt gaataaaaat aaaaacgcat
    17401 tgcaattcaa gacgaataaa atcgtcaagt gatcgactcg acaaaacgaa atcttaattt
    17461 ttaacttatc tatcaataag ttaaaaaaat actattcacc gtgcgaagtt tctttgaaat
    17521 taagactaca gcctgaggct gtggataact ctgtttacaa gagtttgtca gagacaacaa
    17581 aaaccagcaa taacgcatcg cgtcgctgtg gatattttat tgagagaaga atttggagcg
    17641 ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg tcgtgctcta ccaactgagc
    17701 tattcccgct tgggtggtct gtgccttaca gcactttcaa attttggagc gggaaacgag
    17761 actcgaactc gcgaccccga ccttggcaag gtcgtgctct accaactgag ctattcccgc
    17821 ttaatcttcg tctttcgaac cgctactgcc ttggctgcct tcactcacct tagtcacata
    17881 cttttgtaag ttcccaagga ttcgttcagt tgccgccttg tcgcaattcg aaatactgcg
    17941 attgggtggt ctgtgctttg cagcactttt caaattttgg agcgggaaac gagactcgaa
    18001 ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact gagctattcc cgcgtactac
    18061 ttaattttgc tttcgtaatt ttcgcatttc tgcgtcgtta cgggaggcgc attatacgag
    18121 aaatctttcc ctctgcaacc ccccaaaaaa gttttttttg cgtttttatc tcaagtgctg
    18181 aaattatcag caagacgttc aatttagcgt caaaatcacg ccgccgcaag tggattccgg
    18241 cgcttacgtg accaggaaaa attttacagc ttgataaaat gctcgcggta gtaagccagc
    18301 tccgccaccg attcacggat atcatccatc gcctgatgcg tcccctgctt ggtaaaacca
    18361 tccagaattt ccggcttcca gcggcgcgcc agctctttca gggtgctgac atcgagataa
    18421 cggtagtgga agtaggcttc cagctccggc atgtatttaa acaggaaacg acggtcctga
    18481 ccgatgctgt taccgcaaat cggcgatttt cccgcaggca cccactgttt taaaaattcg
    18541 agcgttgcca gttcagcttc ccgatcgccc atcgtgctcg ctttcacgcg ctctaccagc
    18601 ccgctggcgg tatgggtgcg cacgttccag tcatccatca gcgccagctg ttcatcagac
    18661 tggtgtactg caatggtcgg cccttctgcc agaatattca ggttggcatc ggtcaccagc
    18721 gtggcaatct caataatgcg atcgcgctcg ggatccagac cggtcatctc aagatcgatc
    18781 caaatcaggt tgttttcatt ggcactcatg ctattttcca cccattcaca gtgactatat
    18841 tgatccacaa ttgcgtgtat catagatgtt ttgcccatca ggggcgacca ggagtctgta
    18901 cgattgagta aaaataaact ctccaaaggc cagcagcgcc gcgtgaacgc caatcaccag
    18961 cgtcgtctta aaacgtctaa ggagaagccc gactacgacg acaatctgtt tggcgagcct
    19021 gatgaaggta tcgtcatcag ccgctttggt atgcacgctg atgtggaatc cgccgatggc
    19081 gacgttcacc gctgcaatat tcgccgtacc atccgttcgc tggtaaccgg cgaccgcgta
    19141 gtctggcgtc cgggtaaacc ggcggcggaa ggcgtaaatg tcaaagggat cgtggaagcg
    19201 gtgcatgagc gtacctcggt gttgacgcgt ccggatttct acgacggcgt aaaacctatt
    19261 gccgccaaca tcgaccagat tgtcattgtc tccgccattt tgccggagct gtcgctcaat
    19321 attatcgacc gttacctggt ggcctgcgaa accttgcaga ttgagccgat tattgtgctc
    19381 aacaagatag acctgctgga cgacgaaggc atggcgttcg tcaacgagca gatggatatc
    19441 taccgcaata tcggttatcg cgtattgatg gtttccagcc atactcagga tgggctaaaa
    19501 ccgctggaag aggcgttgac cgggcgcatc agcatttttg ccgggcagtc tggcgtcggc
    19561 aaatccagcc tgctgaatgc gttgctgggg ctgcaaaaag agatcctgac caacgatgtc
    19621 tctgacaact cggggctcgg tcagcacacc acgaccgccg ctcggctgta tcacttcccg
    19681 cacggcggtg atgtgattga ttcccctggt gtgcgtgagt tcggcctctg gcacctggag
    19741 ccggaacaaa tcactcaggg ctttgtcgaa ttccatgact atttaggtct gtgtaaatat
    19801 cgcgattgca aacacgatac cgatccgggc tgcgctatcc gggaagcggt tgaggaaggg
    19861 aaaatcgcgg aaacccgttt cgaaaactat caccgtattc tggaaagcat ggcgcaggta
    19921 aaaacgcgta aaaacttttc tgatacggat gactgacaat taagctaacc gtcgttagaa
    19981 tcgtcccctt tttttcagga tccggcatgt tggccggatc aggaacgaca aaacaatggc
    20041 ctggaggcta ccttgttaaa ttcatttaaa ctttcgctac agtacattct gccgaaacta
    20101 tggcttactc gcctggcggg ttggggcgca agcaagcggg caggatggct gacaaaactg
    20161 gttatcgatc tgttcgttaa atactacaag gtcgacatga aagaggcgca aaagccggac
    20221 accgccagct accgcacctt taacgaattc tttgtccgtc cgctgcgtga cgaagtacgc
    20281 ccaatcgata ccgatccgaa tgtcctggtc atgcctgccg atggcgttat cagccagttg
    20341 ggtaaaatcg aagaagataa aatcctgcaa gccaaaggcc acaactacag cctcgaagcc
    20401 ctgctggcag gcaactatct gatggcggac ctgttccgca acggtacgtt tgtgaccact
    20461 tacctctccc cgcgtgacta ccatcgcgta cacatgccgt gcaacggtat tctgcgtgag
    20521 atgatctacg tgccgggcga tctcttctcc gttaaccatc tcaccgctca gaacgtgccg
    20581 aatctgtttg cccgtaacga acgcgtgatt tgcctgttcg ataccgaatt tggcccgatg
    20641 gcgcagattc tggtcggggc gacgattgta ggcagcattg agacggtctg ggcgggcacc
    20701 attacgccgc cgcgcgaagg tatcatcaag cgctggacct ggcctgccgg ggaaaacgac
    20761 ggttctgtgg cactgctgaa aggccaggaa atgggccgct ttaaactcgg ctctaccgtt
    20821 atcaacctgt ttgcaccggg taaagtgaat ctggttgagc aactggaaag cctgtctgtc
    20881 acgaaaattg gccagccgct ggcagtatct accgaaacct ttgttacgcc agacgctgaa
    20941 cccgccccac ttcctgctga agagatcgaa gcagaacacg acgccagccc attggttgac
    21001 gataaaaaag accaggtcta atccatcaaa ggaaacgctg acgtgcgcct gattatcact
    21061 tttctgatgg cctggtgcct cagttggggg gcgtacgccg cgacggcccc cgatagcaaa
    21121 caaatcactc aggaactgga gcaggcaaaa gcggcgaaac ccgcacagcc ggaagtcgta
    21181 gaggcgctcc agtctgcctt aaatgcgctt gaggaacgaa aaggttccct tgagcgcatc
    21241 aaacaatatc agcaagttat cgataattat ccgaaactct ccgctactct gcgcgcacaa
    21301 ttaaacaaca tgcgtgacga gccgcgcagc gtgtcgccgg gaatgtctac cgacgcgctg
    21361 aatcaggaaa ttctccaggt cagcagccag ttgctggata aaagccgtca ggcccagcaa
    21421 gagcaggagc gcgcccgcga gattgccgat tcgctgaatc aactgccgca acagcaaacc
    21481 gacgcccgcc gtcagttaaa tgagatcgag cgccgcctgg gaacgcttac tggcaatacc
    21541 ccgctcaatc aggcacaaaa tttcgcgttg cagtctgact ccgcacgcct gaaagcgctc
    21601 gttgatgaac tggaactggc gcagctgtct gccaataacc gtcaggaatt agcgcgctta
    21661 cgctcagagc tggcggaaaa agagagccag caactggatg cgtatttgca ggccttacgt
    21721 aatcagttga acagccaacg tcagcttgag gcggagcggg cgctggaaag taccgaattg
    21781 ctggcagaaa acagcgccga tttgccgaaa gacatcgtcg cgcaattcaa aattaaccgc
    21841 gaactatcgg cggcactgaa tcaacaggcg cagcggatgg atctcgttgc ctcgcaacag
    21901 cgccaggcca ccagccagac gttacaggtt cggcaggcgc tgaatacgct gcgtgaacag
    21961 tcgcaatggc tgggatcgtc caacctgctc ggcgaagcgt tgcgggcgca ggtggcacgg
    22021 ctgccggaaa tgccgaaacc acaacagctt gataccgaaa tggcgcagtt gcgtgtgcaa
    22081 cggttacgtt atgaggatct gcttaataaa cagccgctgc tacggcaaat tcatcaggcc
    22141 gacggtcagc cgctgaccgc cgagcaaaac cgtattctgg aagcacagct acgcactcag
    22201 cgtgagttgc tgaactcatt gttgcagggt ggcgacacgc tactgctgga actgaccaag
    22261 ctgaaagtct ccaacgggca acttgaggat gcgctgaaag aggtgaatga agcgacgcat
    22321 cgctatctgt tctggacctc tgacgtgcgc ccgatgacca tcgcctggcc actggaaatc
    22381 gcccaggatc tgcgtcgtct catttcgctg gacaccttca gtcagttggg caaagccagt
    22441 gtgatgatgc tgaccagcaa agagacgatt ttgccgctgt ttggcgcgtt gattctggtc
    22501 ggttgcagta tttactcgcg ccgctatttc acccgttttc ttgaacgttc ggcggcgaaa
    22561 gtcggcaaag tgactcagga tcacttctgg ctgacgttgc gcactctttt ctggtcgatt
    22621 ctcgtcgcgt caccgctgcc ggtgctgtgg atgacgctgg gttacggctt gcgcgaggcg
    22681 tggccttatc cgctggcggt cgcgattggc gatggcgtaa cggccaccgt gccgctgctg
    22741 tgggtagtga tgatttgcgc cacctttgcc cgcccgaacg gcttgtttat cgctcatttt
    22801 ggctggccgc gcgaacgtgt ttcccgtggg atgcgctact acctgatgag catcgggctt
    22861 attgtgccgc tgattatggc gctgatgatg ttcgataacc tcgacgaccg tgaattctcc
    22921 ggttcgctgg gacggctttg ctttatcctc atttgcggtg cgctggcggt ggtcaccctc
    22981 agcctgaaaa aggccgggat cccgctgtac ctcaacaaag agggcagcgg cgacaacatt
    23041 accaaccata tgctgtggaa catgatgatt ggcgcaccac tggttgccat tctggcgtca
    23101 gcggtgggtt atctggcaac ggcgcaggca ctgttagcga ggcttgaaac ctcggttgcc
    23161 atctggttcc tgctactggt ggtttatcac gttatccgcc gctggatgct gatccagcgt
    23221 cgcaggctgg cgtttgaccg ggcgaagcat cgccgggcag agatgttagc gcaacgcgcg
    23281 cgtggcgaag aggaagcgca tcatcacagt agcccggaag gggcaattga agtcgatgaa
    23341 agcgaagtcg atctcgatgc catcagtgcg caatccttgc ggctggtgcg ctcaattttg
    23401 atgttgatcg ccctgctttc tgtcattgtg ctgtggtcag aaatccattc cgctttcggc
    23461 ttcctcgaaa atatttcgct gtgggatgtc acctccacgg tacagggcgt agaaagtctg
    23521 gagccaatta ccctcggtgc ggtgctgatt gccattctgg tgtttatcat caccacgcag
    23581 ctggtgcgca acctgcccgc gctgctggaa ctggcgattt tgcagcacct ggatttaacg
    23641 ccgggtacgg gctacgccat caccaccatc accaaatatc tgctgatgct gattggcggg
    23701 ctggtcggct tctcgatgat tggtattgag tggtcgaaat tgcagtggct ggttgccgcg
    23761 ctcggtgttg gtctcggttt tggtttgcag gagattttcg ccaactttat ctctggcctg
    23821 attatcctgt tcgaaaaacc gattcgcatt ggcgatacgg taacgatccg cgatcttacc
    23881 ggtagcgtga cgaaaattaa cacccgcgcc accaccatca gcgactggga ccgtaaagag
    23941 ataatcgtgc cgaacaaggc gtttattacc gagcagttta tcaactggtc gctctctgac
    24001 tcggtcacgc gcgtggtgtt gacgataccg gcccctgccg atgccaatag cgaagaagtg
    24061 acggaaatcc tgctcaccgc agcgcgtcgc tgctcgctgg tgatcgacaa cccggcaccg
    24121 gaagtcttcc tggtggatct gcaacagggg attcagattt tcgagctgcg tatttacgcc
    24181 gctgagatgg gtcaccgtat gccgctacgc catgagatcc accagctgat tctggcaggc
    24241 ttccacgccc acggtatcga tatgccattc ccgcccttcc agatgcgtct ggaaagcctc
    24301 aacggtaaac aaacggggag aacgctgacg tctgcgggca aaggtcgtca ggcgggaagt
    24361 ttgtaagttt gtgtgccgga tgaaccaatc cggctcacaa acaaaactga tagcgcagaa
    24421 aaaactgcca cagccagtag ccgaaaatag ccagcgcggt ggcgtacata aaccagccgc
    24481 gtttttttcg ccacaaagcg aaccagagaa aagggaaaaa gagcggtaac gtcattatca
    24541 caccaacatc acgggtatcg tcttcttcga gatacatcaa cacatcgcag acgttgttaa
    24601 tcccgcgtcc atcaatcatc cactctttgt ccagcgcgga agtgatggtt atccagagga
    24661 acgccacaat aaaccagatg cagcataaga caaacatgcg cgcactcata aggtaaccgg
    24721 tcgcttaatt tcgctatagc gcatacgaaa caaatcgccg tatcccacta attccccgcg
    24781 aaaaaccaga catcggataa gataagcgca ataaatatcc ggcaggtttc cccaccgttt
    24841 accatcgcga tctttgaggg tgttaatggt gctacccacc accatcgcca ctttacgggt
    24901 gtggcaggca tcgacaaaag agagaatcgt ctgctctatt tccgctacat ccccggcaga
    24961 aaattgcagc gccctgattt catcttcggt tattgccggg tcccggctgg tgtcatccat
    25021 aacatttcct gtctttacgc cctatcgtca ggtagacgat agggcatcgg gttattccac
    25081 cgcccactgc ggattttttg ccactctgct gcaatagttc tggtaaatcg ccatcgctaa
    25141 cagcgagaag aagacagggc cgccaatcat ccacaatgtg ctgtcccagt ctccggcttc
    25201 aaccacaggt tgaatgatgg tgaagacgtt cgcaaatgtc accaccagta cgacaaccac
    25261 tgttgcaatc attgccgaca tatgcgtttt gaaaatcaca aatggtctgt cgagatcctg
    25321 acgtgcttta aaaaatggga aagccagcgc gaggaacagg taaggaagcg tcatagacac
    25381 gttcgccatc agcgtcagct tgttaaagaa cgccgatgcg gtaccgccac caaacgaaac
    25441 cagcaggatg aagacagtaa ccaacccgca ctgcatccac atggcgatag acggcatccc
    25501 catcgcattc aggcgcgtca tcggttccgg ccacaacgct ttcggcgtcc cctggatgat
    25561 ggctttcagc ggtgaatagc aaagcgtaaa gaacgcaccg gtataagcga ggaacatcga
    25621 aagtccggta atacgcgcaa accatacgcc cagcgacaat gacgcttcag gtgacaaatg
    25681 cagtgcgtta cccagcgtca ccccgaggct cttcatcagc acataggtaa tattgccaag
    25741 gttaacggaa ccattactta atacctgctg ccagtttgtg ctgacgcccc ataaaaatat
    25801 tgccagcgaa taaccgattg aaataacaat agcggcaaaa acaatacctt tggcaaagtt
    25861 cttttctgga ttttccgttt tatcgaccag accaccaacc gcttcaattc cgccataggc
    25921 aaaaatagca aataccacaa acgatagcat tgccagaccg gactgataac ctgggttcgg
    25981 tgatgcaagg aaattaatat cctgcgcgaa atgcccacca tttaataaca aaatagtaat
    26041 gcttactaac agcaatacta aattcagaca cattactgca ataccgccca ccgcagtaat
    26101 gcgggcaatt ttattaatcc cctttgaagc aacgacggtg accagaatca tccatgccac
    26161 tgccagcaga ccaaccacct gcgtaggctc cagtccagca atacgccagt gctgggtcat
    26221 gtcgctacca tagaggaatg ttgagaacgg tacccaaact ttcgcggagg tactcaccat
    26281 ccagatgata taagaggaaa accacataaa cgtaccaatg aaggcaaaac gtggtccgac
    26341 actattattc atccaggaat agataccgcc ttcttctttg cgataagcag ctcccatttc
    26401 agccatcatt aaggcgaatg gaataaagaa taacaatgca gaaaatatat aaaagggaat
    26461 cgcactataa cccattaagt aataagccga tgggctattg gcaaatccaa atacggaagt
    26521 aaagatcatc aatatgagtc ctatcagact catctttttt atcgtgtgag gcatgaaacc
    26581 atccttcatt tcagcgcgtg tataacacgt gcccactgaa ctgcactgcg caacattgag
    26641 caggcagcgt aaaaaccaat acagaattga tacctggatg atatcaaata tccagtctta
    26701 aattgtggcc tttaacggaa aaatgaaaac atatgctaca gaatggcgct tatcacgcca
    26761 ttcttcgctg ttaattcagt aatttttcag aattatgccc ggtcaacgct aaaggcgatg
    26821 acttcagcca gtgtctccgc gcccagcgcc aacatcacca gacgatcaac acctaatgcc
    26881 acgccggaac agtcaggcat accgactttc aaggcttcaa tcagattctg gtcaatgggg
    26941 tgctgcggca aaccgcgcgc cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc
    27001 tcacgggcat ccgtcaattc atggaaacca ttcgccagct caataccttt ataataaacc
    27061 tcaaagcgtt cagcgacccg atgatcttcg gtactgattt gcgccagtga tgcctggctg
    27121 gctggaaagt ggtacacaaa ggtcggtttt tctttgccaa tatttggctc tacgccaaag
    27181 gtaaacagca attgtagcag cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa
    27241 tccagtttcg ctgcgacttc ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc
    27301 agataacgca agaaagcttg ttgataagaa aggctttctg ctgccgggca gtccagcacc
    27361 tgttgtaaga gatcgtccac ctcgttcatc aaccggtaca tatcatagtg cggtcgatac
    27421 cactccagca tagtgaactc agggttgtga taacgcccca tctcttcatt acggaagctg
    27481 cggcacagct ggaataccgg cccacaaccg gcaaccagca ggcgtttcat atggtattcc
    27541 gggctggtca ttaaccagag attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc
    27601 tcaaacggga ccaaatgaat atcggttacc gtcgcctggc tcatacaagg cgtctccacc
    27661 tccagcactc cacgatcggc aaagaaacga cggatctccg ccataatcgc cgcgcgtttt
    27721 aataagttag gaatggatgc gctcggctgc caggatgccg tttcgctcat agttaaatct
    27781 ccagtttttg acaagggcac gaagtctact cgcaacgcga cggcgagaca aattttacgc
    27841 aggaatcaaa cagcggtggg cagtga
//
LOCUS       DAZGJW010000059        27398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZGJW010000059 DAZGJW010000000
VERSION     DAZGJW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.059"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(60..449)
                     /gene="mutT"
                     /locus_tag="ACHKIK_002461"
     CDS             complement(60..449)
                     /gene="mutT"
                     /locus_tag="ACHKIK_002461"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO9083617.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(509..3214)
                     /gene="secA"
                     /locus_tag="ACHKIK_002462"
     CDS             complement(509..3214)
                     /gene="secA"
                     /locus_tag="ACHKIK_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO9083618.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTVEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(3276..3788)
                     /gene="secM"
                     /locus_tag="ACHKIK_002463"
     CDS             complement(3276..3788)
                     /gene="secM"
                     /locus_tag="ACHKIK_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO9083619.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(4019..4936)
                     /gene="lpxC"
                     /locus_tag="ACHKIK_002464"
     CDS             complement(4019..4936)
                     /gene="lpxC"
                     /locus_tag="ACHKIK_002464"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO9083620.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(5037..6188)
                     /gene="ftsZ"
                     /locus_tag="ACHKIK_002465"
     CDS             complement(5037..6188)
                     /gene="ftsZ"
                     /locus_tag="ACHKIK_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO9083621.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(6249..7511)
                     /gene="ftsA"
                     /locus_tag="ACHKIK_002466"
     CDS             complement(6249..7511)
                     /gene="ftsA"
                     /locus_tag="ACHKIK_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO9083622.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(7508..8338)
                     /gene="ftsQ"
                     /locus_tag="ACHKIK_002467"
     CDS             complement(7508..8338)
                     /gene="ftsQ"
                     /locus_tag="ACHKIK_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO9083623.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYMPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNHAQAEQQ
                     "
     gene            complement(8340..9260)
                     /gene="ddlB"
                     /locus_tag="ACHKIK_002468"
     CDS             complement(8340..9260)
                     /gene="ddlB"
                     /locus_tag="ACHKIK_002468"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO9083624.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(9253..10728)
                     /gene="murC"
                     /locus_tag="ACHKIK_002469"
     CDS             complement(9253..10728)
                     /gene="murC"
                     /locus_tag="ACHKIK_002469"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO9083625.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISAYNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(10782..11849)
                     /gene="murG"
                     /locus_tag="ACHKIK_002470"
     CDS             complement(10782..11849)
                     /gene="murG"
                     /locus_tag="ACHKIK_002470"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO9083626.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(11846..13090)
                     /gene="ftsW"
                     /locus_tag="ACHKIK_002471"
     CDS             complement(11846..13090)
                     /gene="ftsW"
                     /locus_tag="ACHKIK_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO9083627.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(13090..14406)
                     /gene="murD"
                     /locus_tag="ACHKIK_002472"
     CDS             complement(13090..14406)
                     /gene="murD"
                     /locus_tag="ACHKIK_002472"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO9083628.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(14409..15491)
                     /gene="mraY"
                     /locus_tag="ACHKIK_002473"
     CDS             complement(14409..15491)
                     /gene="mraY"
                     /locus_tag="ACHKIK_002473"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO9083629.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGIAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(15485..16843)
                     /gene="murF"
                     /locus_tag="ACHKIK_002474"
     CDS             complement(15485..16843)
                     /gene="murF"
                     /locus_tag="ACHKIK_002474"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO9083630.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(16840..18327)
                     /gene="murE"
                     /locus_tag="ACHKIK_002475"
     CDS             complement(16840..18327)
                     /gene="murE"
                     /locus_tag="ACHKIK_002475"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO9083631.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVTAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(18314..20080)
                     /gene="ftsI"
                     /locus_tag="ACHKIK_002476"
     CDS             complement(18314..20080)
                     /gene="ftsI"
                     /locus_tag="ACHKIK_002476"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO9083632.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(20096..20461)
                     /gene="ftsL"
                     /locus_tag="ACHKIK_002477"
     CDS             complement(20096..20461)
                     /gene="ftsL"
                     /locus_tag="ACHKIK_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO9083633.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVEPSQENIVVQK"
     gene            complement(20458..21399)
                     /gene="rsmH"
                     /locus_tag="ACHKIK_002478"
     CDS             complement(20458..21399)
                     /gene="rsmH"
                     /locus_tag="ACHKIK_002478"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO9083634.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(21401..21859)
                     /gene="mraZ"
                     /locus_tag="ACHKIK_002479"
     CDS             complement(21401..21859)
                     /gene="mraZ"
                     /locus_tag="ACHKIK_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO9083635.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(22461..23465)
                     /gene="cra"
                     /locus_tag="ACHKIK_002480"
     CDS             complement(22461..23465)
                     /gene="cra"
                     /locus_tag="ACHKIK_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO9083636.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(23547..23633)
                     /locus_tag="ACHKIK_002481"
     CDS             complement(23547..23633)
                     /locus_tag="ACHKIK_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO9083637.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(23645..24136)
                     /gene="ilvN"
                     /locus_tag="ACHKIK_002482"
     CDS             complement(23645..24136)
                     /gene="ilvN"
                     /locus_tag="ACHKIK_002482"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO9083638.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(24139..25863)
                     /gene="ilvI"
                     /locus_tag="ACHKIK_002483"
     CDS             complement(24139..25863)
                     /gene="ilvI"
                     /locus_tag="ACHKIK_002483"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO9083639.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            25982..26026
                     /locus_tag="ACHKIK_002484"
     CDS             25982..26026
                     /locus_tag="ACHKIK_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083640.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(26181..27125)
                     /gene="leuO"
                     /locus_tag="ACHKIK_002485"
     CDS             complement(26181..27125)
                     /gene="leuO"
                     /locus_tag="ACHKIK_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO9083641.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         6556 a         7798 c         6568 g         6476 t
ORIGIN      
        1 cagcgtcgca tcaggcatct tgtgcaaatg tcggatgcgg cgtgaacgcc ttatccgacc
       61 tacagacgct taagcttcgc aattaccggt tcattggctg gcggaaaatc atcggcatta
      121 agaccgacta gcgacatcca ctcaccgggt tgcccttctt taccccacgg ctccccttcc
      181 cagctttcga ccagccagaa ccacagagtt atatgcctgt ccgggaattc atattccagt
      241 ttttcaaata gcgaaaaatg ttgtggggta atcccgactt cttcctgaag ttcacgcacc
      301 accgcctgtt ccggcgtttc gcccatttca attttaccgc cgggaaactc cagtttattc
      361 gccatgtgcg catctgctgc gcgacgcgtt ataaagattt cattgttctc gttgcgaata
      421 atacctaccg caatttgcag ctttttcatt gtcttaaacc tataaaaaag gcgcagaatc
      481 ctgcgccttt tacttcaact gttagctttt attgcaggcg accatggcac tgcttgtatt
      541 ttttaccaga accgcacggg caaggatcgt tacgtcctac tttgcgctca ccggtttgcg
      601 ccgccagtgc agctgcggct gcagagtcgt catcctgatg gctaagctgc tgcatttgcg
      661 ctaaacgctc ggcttccata cgacgctgtt gttccagctc ctcaacctct tcaggcatac
      721 gtacctggac tttgctcagc gtgctgataa cttcatattt taacgactcc agcatcgctg
      781 caaacatgga gaacgattca cgtttgtatt cctgcttcgg atctttctgt gcatagccac
      841 gcaggtggat accctgacgc agatagtcca tcgctgccag gtgctctttc cacagggagt
      901 caagcgtttg cagcatgacg cctttctcga agtgacgcat catctcagca ccaaccactt
      961 cttctttacg ctgatacact tcgatggact gcgccagaat gcgctcacgc agcgtctctt
     1021 catgcagttc tggttcttta tccagccact cggcaattgg taaatcgagg tcgaaatcgt
     1081 tcttcagacg ttcctgcagc cccggaatat cccacatttc ttccagcgac tgtggcggaa
     1141 tgtaggcatc aatggtggct ttgaacacat cttcacgaat gctgttgatg gtttcgctca
     1201 catcgctgac atccagcagt tcgttacgct gggagtaaat ggcgcgacgc tgatcgttag
     1261 ccacgtcatc atattccagc agttgcttac gaatgtcgaa gttacgactt tcaactttac
     1321 gctgggcgtt ggcaatcgct ttagtcaccc acgggtgttc aatggcttcg cctggcttca
     1381 tacccagttt acgcatcatg ccggatactc ggtcggaagc aaaaatacgc atcagcgcat
     1441 cttccatcga caggtagaaa cgggaagaac cagcatcccc ctgacgacca gaacgaccgc
     1501 gcaactggtt atcgatacga cgggattcgt gacgctcggt accgatgata tgcaggccac
     1561 ctgcttccag taccgcatcg tgacgtacct gccagtcggc tttaattttt tcaatttgct
     1621 ctacggtcgg attttccagc gcggcaactt ctgcctgcca gctaccaccg agcacaatat
     1681 ctgtaccacg acccgccata ttggtcgcga tagtcaccgc agccggataa cctgcctgag
     1741 caacaatcgc cgcttcgttg gcgtggaatt tggcgttcag gacgttgtgc ttaataccgg
     1801 ctttggtcag ttcgtttgac accagctccg atttttcgat ggagatagta cccaccagca
     1861 ccggctggcc tttcgcagta cgttctttga tatcttcaat gatcgcctga attttttccg
     1921 cttcagtcat gtagaccagg tccggcagat ctttacgaat cattggacgg ttggtcggaa
     1981 caacgacggt atccagcttg tagatggagc taaattcgaa agcttcggta tcagcagtac
     2041 cggtcatccc cgccagtttt tcatacagac ggaagtagtt ctggaaggtg atcgaagcca
     2101 gcgtttggtt ttcgttctgg atctgcacac cttctttcgc ttccacagcc tggtgcagac
     2161 catcggacca gcgacggccc tgcatggtac gaccggtgtg ttcgtcaacg atgataactt
     2221 caccatcttt aacgatgtag tcgacgtcgc gggtaaacag cgcatgagcg cgcagcgccg
     2281 ccgttacgtg gtgcatcagc atgatgttgg ccggagagta cagagactcc ccttcatcca
     2341 tgatgccctc tttcaccagc agttcttcaa tcagcaccag accacgttcg gtcaggttca
     2401 cctggcgaga tttttcgtcc accgagaagt ggccttcgcc ctggaaggtt tcggagtctt
     2461 ctttttcctg acggatcagg tgcggaataa ttttattcac gcgtttatac atttccgagc
     2521 tgtcttctgc cgggccggaa atgatcagcg gtgtacgcgc ttcatcgatc aggatggagt
     2581 ccacttcgtc caccagcgca tagtgcagtt tacgctgtac acgttcttca gggctgaacg
     2641 ccatattgtc gcgcaggtag tcaaagccgt attcgttgtt cgtaccgtaa gtgatgtcag
     2701 ctgcgtaagc ttcgcgcttt gccggtgctg gcatgcccgg caggttgata ccgacagtca
     2761 ggccaaggaa ttcaaacagc ggacggttgt tttcggcgtc acgttgcgcc aggtagtcgt
     2821 tgacggtaac tacgtgcacg cctttaccgg ttagtgcgtt caggtaagca ggcagcgttg
     2881 cggtcagggt tttaccttca ccggtacgca tttcggcgat gcagcgttcg ttaagaacca
     2941 taccgccgag taactgaacg tcgaagtgac gcataccaaa gacgcgctta cttgcctcac
     3001 gtaccacggc gaaagcttcc gggatcagat tttccagcac ttcgcctttt tccagacgtg
     3061 cacgaaactc tgcggttttc cctttcagtt cttcgtcgga gagtttttcc atctccggtt
     3121 ccatggcatt gatgatgttg accactttgc gcatccggcg cagggtgcga tcgttacgac
     3181 taccgaaaac tttagttaac aatttgatta gcataataaa atctcaaacg ccccgcgttg
     3241 cggagttaat aaaatgaagt aaaggtttat tgttgttagg tgaggcgttg agggccagca
     3301 cggatgcctt gcgcctggct tatccagacg ggcgtgctga attttgcttg tggggtaaaa
     3361 tgcgcataat caatgcgata acccttttca gacggcgtgc cttcctgggt cagcagcgcg
     3421 ctgagcgtat ccagtaatgc aagatgttgc gcctgaagag gcaaagattc ttcagcaacg
     3481 ggcagtgttt gcggtgccat tgcgaaagaa agatgacgga ttaccgtgcg aatggcatgt
     3541 tgatgccagt aatcaacgga atagttcgaa ttcgggcggc gtgtgttcgc ttccagcaag
     3601 gccaattgac caaagttaac tttggctgaa ggctcgtggt tgcgggttgt cgcttttgcg
     3661 ggcgcgtttg gttcggcggc gttgctgagc gcaggcaaac ctaaactcgc cgcaaccatc
     3721 cctaataaga gatgcggcca gaagtagcgt ttaccaaact gtcgccagcg cgtcagtatt
     3781 ccactcacgt tattgccatc ccgatcgtat tcaaacgtat ttagcgcaca aatcttatca
     3841 ttaaagcctg cagtccacag cacgaatgaa gataagttgt gcggaaaagt gcttaagaaa
     3901 acagcgttcg caccgttttt tatagctctt ataaaattta aaaaaaaacg actggttcac
     3961 ctggccggag agagtgccag atttgccagt cgaattttat acgacagtat aaatgtcgtt
     4021 atgccagtac agctgaaggc gctttgaagg ccaacggcag ttctgcgtcg tcctggaagg
     4081 tcacatattc ccaggcttcc tgtttcgcca ggacagcctg cagcagtttg ttattcagtg
     4141 catgaccgga tttataagcg gtaaatgcac caataatatt gtgaccacac atgaacaagt
     4201 caccgatcgc atcgagcatt ttgtgacgca caaattcgtc ttcaaaacgc aggccgtctt
     4261 cgttcagtac gcgataatcg tcaacaacga tggcacaatc gaagctgccg cccaggcaca
     4321 aaccacggga ctgcagatat tcgatatcac gcatgaaacc gaacgtacgc gcacggctga
     4381 tctggcgcat aaacgcatca gcggagaagt tcatcgcata gcgctggttg ctggaatcaa
     4441 tagccggatg gttaaaatcg atggtgaaat ccagcgaaaa accattgtac ggcttaaatt
     4501 cagcccactt atcgccatct tcgacacgaa cagtctcttt gatgcgaaca aattttttgg
     4561 cgcagttcaa ctcgtcgata ccggcgtcaa gcagcaggta tacaaacgga gcggcgctgc
     4621 cgtccatgat cgggatttcc ggcgcgttaa cttcgataac aatgttatcg atgcccaagc
     4681 ccgcgagagc agcattgagg tgctctacgg ttgaaatccg tacatcatgc tcgttgacca
     4741 gacacgtaca gagcatggta tcacgcacag atttggcatc ggccgggaaa tctaccggtg
     4801 gattcaagtc ggtgcgacga tagatgaccc cggtgttggc cggcgcaggg cgtaacgtca
     4861 gggtgacttt cttgccggta tgtaaaccga cacccgtcgc ctgaacgata cgtttaagtg
     4921 tcctttgttt gatcatcgta ttatctcgcc aaattaccta tccaaccgaa gtgtactata
     4981 cattcggcgg gccagtttag cacaaagagc ctcgaaaccc aaattccagt caattcttaa
     5041 tcagcttgct tacgcaggaa tgctgggata tccagataat ccggctcttt cgcagtttgc
     5101 ggcgcattgt cattcacgac tttagcaacc ggcttctgct cctgggtcag cggagccatc
     5161 ccatgctgct ggtagcgatc catcactggc tgctgaacct gcttattggt caccagagtg
     5221 atttcaggac gtttgtccat gccgatacct gtcgcaacaa cggttacgcg cagctcgtca
     5281 ttcatatccg ggtcaagaga agtaccgata accacagtcg cgttgtcgga agcaaatgca
     5341 cggatggtgt tacctaccgt ttcgaactca tccagacgca ggtcgaagcc cgccgtgatg
     5401 ttaaccagca cgccgcgcgc gccagacagg tcgatatctt ccagcagcgg agaagagata
     5461 gccatttcag cagcttcttc cgcacggtct tcaccgctcg ccacgccaga acccatcatt
     5521 gcgtagccca tctcagacat tacggtgcgt acgtctgcaa agtccacgtt catcaaaccc
     5581 ggacgagtaa tcagttcagc gataccttgc acagcgcctt tcagtacatc gttcgctgcg
     5641 ccaaacgcat ccagcaggga gataccgcgg cccagaactt tcagcagttt gtcgttcggg
     5701 atagtgatca gagagtccac atgcttggac agttcagtga tcccctgctc cgcgaatgcc
     5761 atacgcttct tgccttcaaa gttgaaaggc ttagtgacga cagcaacggt caggataccc
     5821 aaatcttttg ccacttcagc gacgactggt gctgcacctg taccggtacc accacccata
     5881 cccgcagcaa taaagaccat gtctgcacct tccagcgccg cacgcaatgc atcgcgatcc
     5941 tcatcagccg cattgcggcc aacttctgga ttagcgccag cgcccagtcc tttggtgata
     6001 ccgctaccga tttgaatcgt ctgtccaacc gctgttttac gcagcgcttg tgcatcggta
     6061 tttaccgcga agaattcaac accttcaatg cgctcgcgca ccatgtgttc aacagcatta
     6121 ccgccgccgc cgccgacgcc gatgacttta atcaccgcgt cattggtaag ttccattggt
     6181 tcaaacatag tttctctccg atttgtgcct gtcgcctgag gccgtaatca tcgtcggcct
     6241 cataaaaatt aaaactcttt tcgcagccaa ctattgagtc gcttgatcca cgagccaact
     6301 gatgctgtaa cacgtttttc tacttcagct tcaccgttaa gatgtgactc tttcccatag
     6361 tgaagcaatc ccaccgccgt cgaataatac ggctcctgag cataatccgt taaaccggta
     6421 atgttcagcg gcgcgccgat acgcacttgc gtatgaaaca cgcgctgagc acaggctgca
     6481 agaccttcga tctgcgctgc gccaccggtt aatacaatgc ctgccgccag gtgatgttta
     6541 accccttgtt ggcgaagctt ttcctgcaac tgcaatatct cttcgttgac caggttgagc
     6601 agctcggtat agcgcggctc gatcacctct gccagggtct gacgttgcag actccgcggc
     6661 ggacgaccac ctacgctcgg cacttccacg ctctcatctt ttccaacgat ggaacccagc
     6721 gcacaaccgt ggcgaacttt aatcgcttcg gcgtcgcttg gcggcgtgcc aaaggcgtaa
     6781 gcgatatcac tggtcacgac attgccagca taaggaatta ccttagtgtg gcgcaatgcc
     6841 ccaccggtat aaacggcgat atccattgta ccaccaccga tatcgacgac gcagacaccc
     6901 agttcacgtt catcttccgt caataccgaa taacttgatg ccagtccggc aaatatcagt
     6961 tggtcaactt tcagcccaca acgttcaacc gctttgacga tgtttttcgc catatcgttg
     7021 tgacatgtga tcaggtgcac ttttgcctgc atccgcacgc ccgaaagtcc taccggattc
     7081 ttgatccctt cctgatagtc aatcgcatac tcttgcggga tcacatgcag cacacgatgc
     7141 tcatcgcgca cacgcaccga tttcgcggta tggacgacgt tttccacatc ttcttgcgtc
     7201 acttcttctt cagaaatagg caccatacca atttcattct ggcagctgat gtgcttacca
     7261 gaaagcgcca gatataccga agagatctga caatctgcca tcaattctgc ctggtcaatg
     7321 gcgcgttgta cgcacttgac cacggattcg aggtcgttca ccccgccttt atccatacca
     7381 cgtgacgggc agctgcccac gccaatgata ttgaccatac cgtcgggcag aacttcccct
     7441 actaaagcgg caaccttcgc ggtaccaatc tccagtccta ctaccagttt tctgtccgtc
     7501 gccttgatca ttgttgttct gcctgtgcat gattttgttg ctgagtagat tcctctggcg
     7561 gcaagggcgc ccagcctact gccgctccag agtcataacg caaatcaacg tagctaatcc
     7621 gtttgccatc ggtttgcgcc tgctgctgta aaaccggata aagttctaca aagcgagcca
     7681 aacgtttcat cgtatcgccc cggccaagat tgagcttaat atcgttattc agcgtcaact
     7741 gccaggaacg ccgcgcggtc atcgccgctt ccttcagagt aaatctgtcc tttgccagca
     7801 tctgccccat ttcgcgatag ccctgcaaca cttcattggc gctgccttcc gggccataca
     7861 gcattggaag cacctgcttg ctggtgcgat ctggcggcac gctgaaggtg tttccttccg
     7921 cgtctaccat atgttgatca ttccaccgcg caatcggcat atattcaacc agatgaatct
     7981 tcaattcatc aggccactgc tttctgacgc tcacctgctt aatccacggc aggcgttgtt
     8041 ctatttgcgt ctggatgatg ttgacatctt gggtcataaa ggtacccggc tcacccaatg
     8101 ccaggatcga ctgccggata tcgtcattac gcgtgtaatg gcgttcaccg gtcaacacca
     8161 gctttgagag cggcaggcgt tgcgcatctt ccatccagcc caacacgacc cagccgctca
     8221 ccaacactgt cgttaaaacg gtcagcagga aaaggatccc cgccagacgc gttccattat
     8281 tgcggcgaga agaaacctct tcttcgctat ttcgcgtgtt cagagcagcc tgcgacatat
     8341 tagtccgcca gttccagaat tcgtactacc aactgcgaga agctcatacc tgcctgacgt
     8401 gccgccatcg gcaccaggct gtggctggtc atacccggtg aggtattggc ttccagcaga
     8461 taaaactgtc catcgctgtc cagcataacg tcaatacgtc cccatccttt gcaacctaac
     8521 gtcgtccatg ctttcagcac taatgcctgc aaattggcct cttgtgacgc ttccagacct
     8581 gcggggcaga aatactgtgt ctcatcagag agatacttcg cctcataatc atagaaggtt
     8641 ccggacggtt gaatacgtat tgacggtaaa atttcttcac cgagtatcgc aaccgtgaac
     8701 tccggcccac ttagccattt ttcaatcaat acttcttcat cgtgctgaaa tgccaatctt
     8761 aatgcatctt gtagagcatt ttctgctact acttttgaca ttcccacact ggaaccttcg
     8821 cggctcggct taacgataac cggcaaaccc agagcagaaa tttctgctaa ctgcttatcg
     8881 ctcaggcctt tttcaaactc tgcgcgggtt aacgctaccc acggcgcgac cggtaaaccg
     8941 gcaccttgcc acagcagttt gctgcgtagt ttatccattg aaagcgcaga tgccatcact
     9001 ccgctgccgg tataaggcaa gcccatcagc tcgagcatgc cctgcagcgt accatcttca
     9061 ccgccgcgac cgtgtagcgc gataaacact ttctgaaaac ccatcgactt cagttgcgtc
     9121 acgtcgactt ctttcgggtc gacaggatac gcatcaatac cgccttcacg cagcccggct
     9181 aacaccgctg cgccagaatt cagagaaact tcccgctcag cggaagtccc gcccaacagg
     9241 accgcgattt tatcagtcat gttgttcttc ctccggagtt tgcggcttca gtttgatttc
     9301 agctaaagaa cgggcaattt ttccgatatt accagccccc tgaacgagaa tcaggtcgtt
     9361 accggttaat accggtgcca gcatctcggc tacctgcgca gggtccggca ccagaatggg
     9421 atcaattttc ccacgtccac gaattgtgcg acacagcgaa cggctgtccg ctcccggaat
     9481 tggcgcttcg ccagccggat acacttccag catcaacagg gtatcaacct gcgtcagcac
     9541 attggcgaaa tcatcataca ggtcgcgcgt acgggtaaaa cggtgcggct gaaacagcat
     9601 taccaggttt ttatccggcc agcctgcgcg cgccgcttta atggtggcgt caacttccgt
     9661 cgggtggtgg ccgtagtcat cgaccagcat tgccgtaccg cttttaccat tcactggctc
     9721 cagcgggaat tcaccgagga aatcaaaacg gcgcccagtc ccctggaagc tctccagcgc
     9781 acgtaaaata gcctcgtcat caattccctc ttccgtagca accgcaaccg cagctgcggc
     9841 gttcagcgcg ttatgacgac ctggcgcatt cagggtgacg cgcatcggct ctttgtcctg
     9901 gcgcagcagc gtaaagtgcc cctgcgggcc aatctgctga taatcttcta cacgcacgtc
     9961 ggcatcttcg ctgaagccgt aagtcgtggt ctgacgtccc actcgcggta acaattcgcg
    10021 gatcaccgga tcatcaacac acatcaccgc acgaccgtaa aacggcaggt tgtgcagaaa
    10081 attaataaaa gtctgtttta aattctcaaa gtcgccctgg taggtatcca tgtggtcggc
    10141 ttcgatattg gtgacaatcg ccaccatcgg ttgcagatgc aggaacgatg catcactctc
    10201 atctgcttcg gcaatcaggt accgaccatg ccccaaacgc gcatgaaccc ccgccgcttt
    10261 taccagcccg ccgttaacga aggttgggtc gagccccgct tctgcgtaga tgctggaaac
    10321 catcgcggtg gttgtcgttt tgccgtgcgt tcctgcaatg gcgatgccat gacgaaaacg
    10381 cattaactca gccagcattt cggcacgacg gatcaccgga atacgcgctt catgagctgc
    10441 gacaatttcc gggttatagg cagaaatggc gctggaaaca acgaccacgc tggcatcacg
    10501 tacgttttcc gggcgatggt tgaaataaat cgtcgctccc agattcatta actgctgcgt
    10561 gaccggattt ggcgctaaat cggaaccact gatctgataa ccttcattgg ccagaacttc
    10621 ggcaataccg cccataccgg caccaccgat gccgacaaaa tgtatgtgcc gaacgcgacg
    10681 catttcgggc acgatggaac gcagttttgc caattgttgt gtattcattc tttacgccat
    10741 taacttctta aattcatacg atgcaaaagg catcgctaca attacgcccg ggcagcccgg
    10801 ctcacttcat ttgccactcg ctcggtggca tccggaatgg atgcagcgcg ggcgcgttct
    10861 gccatggtta ataaggtttc tcgcgaccac ccggccaggg tgttggcgac agcatccacg
    10921 ctaagctgtg gctgctcgat aattttggct gcgcccgctt tttccagcgg tagcgcattc
    10981 cagtattgct ggcggtcttt atgttgaaac ggcacaaaca acgccggtag tcctgccgcg
    11041 gcgatttcac tcaccgttaa agcaccggag cggcaaacga cgacatccgc ccacgcatac
    11101 gccgccgcca tatcatcaat aaattccgtc actttatgct gcggttgccc cgcttcggca
    11161 tacgcctgtt caacggattg ttgcgaacct ttaccgctct gatgccagat agtgactgaa
    11221 tcacccagtt tcgcagcaac ctgcggcatt gtctggttaa gaatgcgtgc gccctgggaa
    11281 ccacccacta ccagcacacg aaccggacct tcacgtccag ccaaacgttg ctgcggcaac
    11341 ggcagcgcca gtacatcggt acgcaccggg ttacccacca cttccgcatt agggaaagcg
    11401 cctggaaacg cctgcatcac tttggtggca atcttcgcca gccatttatt ggttaagccc
    11461 gcaataccgt tttgttcatg aagtacaacc ggaatgccta acgaccacgc ggccagacca
    11521 cctggacctg atacatagcc gcccataccg agtaccacgt caggtttgta cgctttcata
    11581 atcgcccgcg cctgacgcca ggcgttgaag atacgcagcg gcgcagctat cagtgctttt
    11641 ataccttttc cacgcagacc agagatacga atgaaatcaa tttcgatgcc atgttttggc
    11701 actaagtccg cttccatacg gtcggcagtc cccagccagc gaacttgcca accctgagcc
    11761 attagatggt gcgcaaccgc cagtcccggg aatacatgtc caccggttcc gcctgccatc
    11821 accattaatc gctttccttg accactcatc gtgaacctcg tacaaacgcc tgcgctttct
    11881 ccagacgcgt ttcataatca atacgcaaca gcatcatgat ggctgtcgac ataatcagta
    11941 agctcgaacc accgtaactg atcagcggca atgtcagacc tttggtcggt aacatccccg
    12001 ccgccgcgcc tacgttaacc agcgcctgga agctaaacca gatgccaata gaacaggcga
    12061 gaaaaccgga aaaacggtgg tcaatttcta acgctttgcg gccaatcgac atcgcgcgaa
    12121 aagcgacgaa gaataccatt aaaagtgcca gcaccacacc gacatacccc agttcttcgc
    12181 cgataatggc gaaaataaag tcagtgtgcg cttccggcag atactccagt ttttgtaccg
    12241 agttacctaa accttgtccc caaagttcgc cgcgaccaaa cgccatcagc gattgcgtta
    12301 actgatagcc gctgccaaag ggatcttccc acgggttcca gaatgcggta acacggcgga
    12361 tacggtacgg ttcggcgagt atcagcaaca caaccgctga aatgcccata ccgataatgg
    12421 caatgaactg ccacaatttc gctcccgcca ggaacaacat cgccagcgta gtcacaaaca
    12481 acaccaccac cgtaccaagg tctggctgtg ccagcagtaa cactgccaac accagaatca
    12541 cgcccatcgg tttcaggaag ccgcgcaggt tattacgtac ttcgtcgcct ttacgcacca
    12601 gatagttggc gatataacaa aacagcgaca gttttgtcag ctccgcaggc tggatacgca
    12661 gcaaaccgag atcgatccaa cgcgatgccc ctttaaccga gctacccact accagcacga
    12721 tcatcagcag gatgatagat ccgagcagca tcgtggcact gtagcgttgc cagaactcca
    12781 tcggcagacg cagcgtaatg atcgccagaa tgaatgccag gatcagatag acgccatcac
    12841 gcttcgcgaa gaagaacgga tcgttggtta agcgttgccc tatgggcatt gacgccgagg
    12901 tcaccatgat aaagccaatc gccgcaaggc cgaaggtcag ccacagtaag gtgcgatcgt
    12961 acatgatcag gctgtcggta tctttttccc gcgagcccat cacccagcct tttagcgccg
    13021 tggagatcca gaccaggata ctgaatcctg gcaggcgcgg cattttcagg cgagggagag
    13081 ataaacgcat caacctaact ccttcgccag acgggcaaac tcattgcctc gttgttcaaa
    13141 gttcttgaac tgatcaaggc tggcacaggc cggggagagc agaaccatat cgcccggctg
    13201 aacacgcgga gccagcaagc gcatcgcctg ttccatagtt tcggtttgtt ctgccacttc
    13261 cgggcgtagc gccgccagct gcgcaccgtc acgaccaaaa cagtacaaac gcacgttgtc
    13321 gccattcaga tagcgcgcca gtggactgaa gtccgccgat ttaccatcgc cgcccagcaa
    13381 caaatgcagc gtgccgtcta cctgcaagcc attcaacgcc gcttccgtac tgccgacgtt
    13441 agtggctttc gaatcgttaa tccagcgtac gccattgtgc tccagcacca cttcaaagcg
    13501 atgcggcaga ccggtgaagg tggttaacgc tttcaggctg ctggcgcgcg gcaaaccagc
    13561 tgcgtctgcc agtgccagtg ctgccagcgc attggtgtag ttatgctgcc cggaaagttt
    13621 catctctttc acgttcagca ctttctcgcc tttcactcgc agccaggttt ctccctgctg
    13681 atgattcagg tgatagtcac ccatgttgac gccaaagctg acgcagcgtt catcagcacc
    13741 gcgaatcggc attgttaagg catcatcagc attgacgaca caaactttcg cattttcgta
    13801 aatacgcaat tttgccgcac ggtattgctg caaaccaaac ggatagcggt ccatatgatc
    13861 ttcagtcacg ttcagaatgg tcgccgccac cgcctgtaag ctggaagtgg tttccagctg
    13921 gaagctcgac agttccagca cgtacagttc gcactcagca tcaagcagca tcaacgctgg
    13981 caggccaata ttgccaccca caccaacgtt aacccccgcc gctttcgcca tttcacccac
    14041 tagcgtggtg accgtgcttt tgccgttaga accggtaatc gccacaatcg gtgcttgtgc
    14101 ttcgcggcag aacagctcga tatcgccaac gatttcgatt ccggcatcag cggcagcgct
    14161 taaggatgga tgcgccagtg caataccggg actggcgaca atcagatctg ccgccatcag
    14221 ccattcatca ttcagactgc ccgtgtggcg ttctacggct tcgggtaatt tatccaggcc
    14281 aggcggtgtc atacgcgtat ccataacgcg cggcgtcaca ccgcgagcga ggaaaaagtc
    14341 cacgcaggaa agcccagtga ggcccaggcc gataatgacg acatttttac cctgataatc
    14401 agccatgatt aacgtacctt cagcgttgcc agaccaatca gaaccagcat cagcgaaata
    14461 atccagaaac gcacaatgac gcgcggttcc ggccagcctt tcagttcata gtggtgatga
    14521 atcggtgcca tgcggaaaat acgttgtccg cgcagtttaa aggagccgac ctgcaggatg
    14581 acagaaagcg tttctaccac gaacacgccc cccataatca ccagcaggaa ttcctgacgt
    14641 agcagtacgg cgataatgcc taacgcacca cctaacgcca gcgaacctac atcgcccata
    14701 aagacctgcg ccggataggt gttaaaccac aggaagccca gtcctgcccc gactatcgcc
    14761 gtgcagacaa tgaccagttc cccggcgtgt cgcagatacg gtatgtgtaa atagctggca
    14821 aagttcatgt tgccggtcgc ccacgccacc agcgcaaaac caccggcgac aaatacggtc
    14881 ggcataattg ccaggccgtc gagaccatcg gtcaggttta ccgcgttgcc agtacccaca
    14941 atgacgaagt aagccagcag aatgtagaac agccccagct gcggcatcac atctttaaag
    15001 aatgggacca ccagctgcgt tgcgggcgtg tctttgccgg caaggtacag ggcgaaggcg
    15061 atacccagcg caatgaccga catccagaaa tacttccaac gagcgatcaa ccctttggtg
    15121 tctttacgca ccactttgcg ataatcatca acaaagccaa taacaccgta acctaccagc
    15181 accaccaaca cgcaccagac gtacggattg gacgggtaag cccacagcag tacggagatc
    15241 acaatcgccg tcaggatcat aatcccgccc atggtcggcg taccgcgctt gctgaagtgt
    15301 gattcaggac cgtcgttacg caccacctga ccaaaggaaa gtttttgcaa atgagcaatc
    15361 atacgcgggc ccatccacaa tgagatgaac agcgcggtca gcaggctgac gatggcgcga
    15421 aacgtcagat aggaaaagac gttaaagccg gaataatatt tgaccaaatg ttcggccagc
    15481 caaactaaca tgtcccattc tcctgtaaag cgcgtactac ctcttccatg gcggcactac
    15541 gtgaaccctt aactaaaatc gtaattacct gttgctcagc aatcagtgat ttaagacgcg
    15601 taattaacgc agttttatca gcaaaatgtt cgccaacgcc gctggcggtg ctgatagcat
    15661 ggctttgttt acccacgctt aacacgcggt caataccagc agcttttgcc gcctcgccca
    15721 cctgtacatg gcaggcttcg ctttcagcgc ccagttccgc catatcgccc accaccagca
    15781 cgcggtagcc cggcatttca gccagtacct gaacagcggc ggtcattgaa ccgacattgg
    15841 cgttgtagga gtcgtcgagc agcaactggt tttctgccag ttggatgggg aacagacggc
    15901 ctggaacagc tttcagattt gccagccccg ctttgatagc atcaagcgtt gcgcccacgg
    15961 acatggagag cgcagcggct gccagcgcat tcgcaatatt gtgacgcccc ggcaacggca
    16021 gcagaacatc gacgctaccg gttggggttt gtagggtaaa ttccgtaccg tgcgaggtca
    16081 catggatatt ggtggcggtg aaatcgctgt tggcggcatt gggtgagaaa cgccacactt
    16141 tgcgtgagcc aattacgctc tgccagttca gccagtcgtt gttgtcggcg ttcataatgg
    16201 cgataccgtt ttccggcagg ccgctaaaga tttcaccttt cgctttcgcg acacccgcaa
    16261 gcgagccaaa accttccaga tgcgccgctg ccaggttgtt gaccagcgca gcttccgggc
    16321 gagtcagact cacagtccag gctatttcgc cctgatggtt cgcgccaagt tcaataactg
    16381 cgtaatcgta ttccggcgtt aagcgcaaca gcgtcatcgg tacaccgatg tcgttgttga
    16441 gattgcctgc cgtataaagc gtgttgccgc actggcttaa aatcgccgcc gtcatctctt
    16501 taacggaggt tttgccggag gaccccgtca gagcaaccac gcgcgccgga acttgctggc
    16561 gaacccatgc agccagttca ccaaacgcca gacgcgtatc cttgacgatt aactgcggca
    16621 ggtcgatgtc cagcggacgg ctaaccagta gtgcgcctgc gccgccagct ttcgcctggt
    16681 cggcaaaatc gtgggcatca aaacgttcgc ctttcagggc aacaaacagg cagcccggcg
    16741 tcagttttcg ggtatcagtg gttacagcat caagggtgat atctgcacct tgcagttcac
    16801 cgttgagaat atcggtaagt tggctaaggg ttacgctaat catgcaatca cccccagcag
    16861 acgcgccacc gtgacgcgat cggagtagtc cagacgctga ttgccaacaa tctggtaatc
    16921 ctcatggcct ttgcccgcga ccagtaccac atcattctct ttagcctgca taacggcgca
    16981 agtcaccgct tcagcacggc cttccatcac tttggcatgt ccggcatcta acattcccgc
    17041 cagaatatcg ttgatgatgg cacgcggttc ttcggtacgc gggttatcgt ccgtcaccac
    17101 cgccacgtca gcaaactctt cggcaattgc gcccatcagt ggacgcttac ctttatcgcg
    17161 atcgccacca cagccaaaga cgcaccacag cttgcccgca cagtgcaggc gcgccgcctg
    17221 taaggctttt tccagtgcat ccggcgtatg cgcgtaatcc accaccaccg tcggtttgcc
    17281 tggcgcagtg aacacttcca tacgtccgca aaccggttgc agacgcgcgg cggtttttaa
    17341 cagatcagcc agcgggtagc caagtgccag cagtgtcgcc agcgcgagca gcaggttgct
    17401 gacgttaaaa gcgcccatca gatggctttc aatttcgcca tcgccccaac ttgaactaaa
    17461 gcgaatggtc gcgccgctgt cgtgatagtt cacttcggtc gctttcaacc agcgtccgtg
    17521 acagttcgga ttgatatgat cttccattga taccgcaacc gcgtccggca gttttgccag
    17581 ccagcggcgg cccacttcat cgtcggcgtt aacaatcgcc tgaccacaat gatgttcaga
    17641 ataaagcagc catttcgcgg cttcgtagtg ttccatatca ccatgataat caaggtgatc
    17701 gcggcttaag ttggtaaaga ccgacgccgc aaatttcaat gccgccacac ggtgctgtac
    17761 cagcccgtgg gaggaaactt ccattgcgca aaacgtcgcg ccctgatcca ccagccccgc
    17821 cagctcatgc tgaacatcga ctgccgaacc ggtggtattt tctgtcggga tcactttccc
    17881 cagcagaccg ttaccaacgg tgcccattac cgcgctggtt tcgccaagca gttggctcca
    17941 ctgcgccagc agctgggtag tcgtggtttt gccgttggtg cccgttacgc ctacgagacg
    18001 taaattgtca gagggttcat ggtaaaagcg gcccgccagt gcagataaac gctcgttgag
    18061 ctggctgaga tagatgaccg gtacgccgtg catttcacgg atttcaccgt cggtcgcctc
    18121 acctttcgcc tctgcaataa tggcagtcac accttgcgct atcgcctgcg ggatatatcg
    18181 acgcccgtcc gcctgatgac ctactacagc tacaaagaga tcgcccgccg cagccacacg
    18241 gctgtcgagt gtcatctctc gcagtgctcg cgaaggtgcg tctggcaccc acggagcaag
    18301 aaggtcgcgc aaattacgat ctgccacctg tcccctcgcc ttgattaatc acaaattcat
    18361 ttttatcgcc cgttgtcagc gcatccggct cgatgttcat ggtacgcaat acgccgccca
    18421 tgatggcacc aaagaccggc gctgaaacgg cgccgccgta gtatttaccc gcctgcggat
    18481 cgttgataac aacaaccagc gcgaagcgcg gctgactcgc aggcgcaacg cctgcggtat
    18541 aagcaatata tttattgatg tagcgaccgt ccggcccgac ctttttcgcg gtaccggttt
    18601 taatggcgat acgatagcct ttaatcgccg ccttcacgcc gccgccgcct ggtagcgcca
    18661 cgctttccat catatgcacc acagtgcgga caatggattc cgggaagaca cgttcaccgg
    18721 gaaccggggg gtcaactttg gtaatcgaca gtgggcgata aatgccgtag ctgccgatag
    18781 ttgcgtagac tcgcgctaac tgtaacggtg ttaccattag cccgtagccg aaagagaagg
    18841 tggccctctc tatgtcagac caccgttgtt tttgaggata taagccactg cgttctccga
    18901 ccaaccccaa attggtcgct tttcccagtc caaaacgtga gtaagtatct actaacgctg
    18961 aggacggcat cgctaacgcc agcttggaaa caccgacgtt actcgacttc tgtaataccc
    19021 cggtcagggt taattcgctg tagcgtgcca cgtctttgat ttcgtggccg ttaattcgat
    19081 aaggaatggt attgagtacc gagttttccc gcaccacgcc acgttgcaac gcggtcatta
    19141 ccaccatcgg tttaaccgtt gagcccggtt caaacacgtc ggtgatggta cggttacgca
    19201 tcgcctcttt cggcgtgccg ctcagattgt tagggttgta tgacgggctg ttagccatcg
    19261 ccagcacttc accggtgttg acatccacca gcacggcgct accagattca gccttgttaa
    19321 aggccaccgc gttgttcagt tcgcgataaa ccagcgcctg caggcgttca tcaatactca
    19381 gcgccaggtt gtgcgctgcc tggctgtcag tagaagaaat atcttcaatt acgcgaccat
    19441 agcggtcttt acgcacaatg cgctcacccg gctgcccggt aagccattta tcgaaactct
    19501 tctcaacgcc ctcaatccct tgactatcga cgttagtaaa gccgatgagg tgagcagtca
    19561 cttcgccgga cggatagtaa cggcgagact cttcacgcag atgaatcccc ggcagtttca
    19621 gttttttgat gtagtccgcc atgtcagggt tcacctgacg cgccagataa ataaagcgcc
    19681 ctttcgggtt ggcgttaatg cgggctgaaa gctgatccag cggaatattg agcgcgttag
    19741 ccagcgcctt ccagcggtca ccgacgctga taccgccagc gtcatgcact tctttcgggt
    19801 cagcccaaat cgcttttacc ggcacgctca ccgctaacgg gcgaccagaa cggtcagtaa
    19861 tcatgccgcg ggaggtggaa acttgctgaa cgcgaagaga acgcatgtcg ccctctttca
    19921 ccagcatatc cggggagata acttgtaacc acgctacgcg tccgagcaga aaagccagcg
    19981 ccaggagaat acagccgcat aacaacgcaa aacgccaact gataaagttg gcatgttctt
    20041 cctgacgttt tggtttctgc gttttcgccg ctgctttcat gcgtcgcgtt tatccttatt
    20101 tttgcactac gatattttct tgtgacggct caacatgctg catttgcagc ttttccgtgg
    20161 cgatcctttc cacccggcta tggtcgccga gcgcattctc ttcaaggatc aggttgcgcc
    20221 attcaatgtc taaagcatct cgctccagca ccagttgttc gcgctgagcg gtcagtaaac
    20281 gggtatggtg cgccgtggtt accacagtca ccgccgtcaa aataatgcaa atgaacaggc
    20341 agagtggcag cttcccaaat cgcaaaagat cgtcaccgat aacaccaggc aatgcatggc
    20401 gctcgtggct tcccatcgat cctttaactt tgcttagagc ttctgtcact ctgctgatca
    20461 tgcattcgtc ctctctgcaa tacgcagaac tgaactacgg gcacgagggt tctcagccac
    20521 ctcttcttcg cccggcatta acttgcctag tgctcgcagc tgacggccac ccagtttttt
    20581 gagctgctct tcagtcatcg gtaaccctgc cggaacttgc ggaccgcggc tgttttcacg
    20641 cataaaacgt ttcacaatac ggtcttccag cgagtggaag ctgatgatcg aaagccgccc
    20701 acccggggcc agcacgttga gcgagctttt tagcgcctgc tctatctcct ccagttcact
    20761 gtttacccaa atgcgcaccg cctggaaggt acgggtcgcg ggatgtttaa acttatcttt
    20821 caccggcgtt gcagcagcca cgacttccgc cagttctttg gtgcgggtca tcggctgttc
    20881 gcggttacgc tcgacaatgg cgcgggcaat gcgtttggca aaacgctctt caccataggt
    20941 tttcaatacc caggcgatat cggcttcttc tgcggtttgt agccattcag cggctgactg
    21001 cccacgggtt gggtccatac gcatgtccag cggaccatcg cgcataaagg aaaagccgcg
    21061 ctcagcatca tcaagttgcg gtgaagagac gccaagatcg aggagaatgc cgtcgatctt
    21121 gccgataaga tcgcgctcgg caacgtattc gcccagcgcg gagaaaggtc cgtggatgat
    21181 ggagaagcgc ggatcatcaa tagtcttcgc aacggcgata gcctgcgggt cgcgatcgat
    21241 cgccagcaaa cgcccctctt cgccaagctg cgagaggatc agacgtgagt gaccaccgcg
    21301 accaaaagtc ccatcaatgt agatgccatc aggacggata ttgaggccat taacggcttc
    21361 atccagcagc accgtagtat gtttatagtt ttccatcatt ttatagagac aagtcctgca
    21421 gtcgctccga taagtctccg gtagccaact gctctgcgtc gatatcttcc ttgacctgtt
    21481 gatgccaggt tgtttcatcc cacagctcaa acttgttgaa ctgtccaacc agcatcactt
    21541 cttttgtcag cccggcatgt tgccgcagta ctggcgcgat taacaatcga cctgcgccat
    21601 ccatctgaca ttcgctggca tgacctaaca gtaggcgctg cacacggcgc tcaaccgggt
    21661 tcatgctcga cagacgcgat aatttttgct cgataatttc ccattcaggt agggggtaaa
    21721 gcagcaggca cgggtgatga atgtcaatgg tgcaaaccat ttgaccggca gcgttctcaa
    21781 gcagctgttc ccggtaacgg gtaggcactg ataagcgccc tttgctgtcg agattgacta
    21841 acgttgctcc ccggaacatg ccagcctcac cccttattcc cactttgccc cacaatttcc
    21901 cactgaaagg agtttacgga gcggaggaaa agcttgtcaa gccagtcaca aggcataccg
    21961 aggtaaaaaa cccgatgttt acggctaata tcagcgttgt ttattttacg tccaacgtgc
    22021 gcagcaaatt aacgttatga atagctgtaa gaaaaaatcc ctcatcgtca tcaccgtttt
    22081 aaaatgactc actattttcc acagcaaata taaagtgtca gtttgcgacg cgagcggcat
    22141 tttaggacat atcttccccg gttaacagtc cctgttgcgt ctgtgtggcg cttgatgcca
    22201 gcgggtttgc gcaaaagcaa agaaaagtgc ttgttaaatc ggcaattcat ctctgcatgt
    22261 aacaaaataa tgcaaaaacg cgctgtcgat atcacttaat tattgtgaat ttcagctggt
    22321 gagttaatta acgggcgggt ttaaaagaac ttatcaataa tagtcaggaa tagtcttatt
    22381 tactttaagg atattgatgt ccattcccgt actctacgcg ccagagggaa attcacctgg
    22441 cgcgtatttt tgttcgcggc ttagctacgg ctgagcacac cgcggcgata gagattacgt
    22501 ttaatgcgcg ttaaaccagg ttttggctta cgcggttcgt ccaggcttgc caggacaatc
    22561 tccagcacac gctctgcgac atcgcggtga cgttgagcca ctgccagcac cgggcactgt
    22621 aagaagtcga gcagttcgtt atcgccaaag gtggcaattg ccaggtcaga aggcagttta
    22681 ccatcgcgac gtagcgtgac atccatcacc ccttgcaaca acgcaaacga cgttgtgaac
    22741 agcgcctgcg gcatcggatg cgtttccagc catttttcga ataactgggc agccgcctcc
    22801 cgctcatagc tgttggcata caggaaatgc acttcgcgcg gatcatcttt ccaggcagta
    22861 cggaaacctt gttcacgcag gaagctgaca gaaagctccg gcagcgcgcc aagataaagc
    22921 accgtctcgg cgggaaactt acgtaactct tccgccagca tttcggcatc atcctgatcg
    22981 gcaccaacca cgctggtgaa atgttcacga tcgagggcgc ggtccagcgc gacaatcggg
    23041 aacgggtcgt tagcccagcg ttgataaaaa ggatgctcag gtggcaacga cgtcgaaaca
    23101 ataatggcat caacctgacg ctgtaaaagg tgctcaatgc agcgcatttc gttgtctggc
    23161 tgatcttctg agcaggcaat cagcagttga taaccccgtt gccgcgcctg gcgctcaaga
    23221 tagttagcga tgcgggtata gctggtgttc tccagatcgg ggatcacaag accaatagaa
    23281 cgtgtgcgtc cagcacgaag cccagctgcc acggcgttcg ggtggtaatt gtgctcacgc
    23341 accacagcca tgactttttc aacggttttg tcgctcacac ggtattgctt cgctttgccg
    23401 ttaataacat agcttgcagt ggtccgcgac actcccgcca gccgagcgat ttcatccagt
    23461 ttcacaattg ccccttgcgt aaaatgtaaa aaccatagcc attgtactgg tatgggttaa
    23521 attcattaac atctaagcgc agaaaattaa ctgcggcaac tgcttttatt ccaggttccc
    23581 actgatttcg caaaaaaagc ccaacgtgac atgttgggct gcaaattgcg cactgagatc
    23641 attctcaacg cattatttta tcgccgcgcg aaagtccgac cacaccagag cgagcaactt
    23701 ccacaatttt cgccacatcg cgaatcgatg ctaaaaatgc atcaagctta tcgctggtgc
    23761 ctgctaattg aacggtataa agcgaggggg tgacatcgat aatttgccca cggaatattt
    23821 ccgtattacg tttcacttcg tcacgcccgt aaccgctggc ctgaattttc accagcatga
    23881 tttcccgctc aacatgcgcg ccctgcccca actcactcac gcgcaagaca tcgaccagct
    23941 tgtgtaattg cttttcgatc tgctcaagca ctttttcatc gcccacggtc tggatggtca
    24001 tacgcgataa tgtcggatcg tcggttggcg caacggtcag gctttcaatg ttgtagccac
    24061 gctgggaaaa aaggccaatc acgcgggata acgcgcctga ttcattttcg agtaagactg
    24121 ataatatccg gcgcataatc aggttctctc cgttttgctt aaccacattt catccattcc
    24181 gcccccgcga atctgcatcg ggtagacgtg ctcactgcca tcgacggtaa catcaacaaa
    24241 caccaggcga ttattgcaca cctgttccag cgcctcgcta agtttgcttt ccagctcatg
    24301 cggatgagag atctggatcc cgacgtgacc ataggcttcc gccagacgga cgaaatcggg
    24361 tagtgattgc atataagatt gtgaatgacg gccggaatag atcatgtcct gccactgttt
    24421 caccatcccc agatagcggt tattgagatt caccaccagt accggcaact cgtattgcaa
    24481 cgcggtagac agttcctgga tgttcatctg aatactgccg tcgccagtga cgcaaaccac
    24541 ggtttcttct ggcaacgcca ttttgacgcc cagtgccgca ggtaaaccaa aacccatcgt
    24601 gccgaggcca ccggaattga tccagcgacg cggtttgtcg aatggataat aaagtgcagc
    24661 aaacatctgg tgctgcccga catcggacgt cacgtaagcg tctcccttcg tcaaccgcca
    24721 aagagtctcg atcaccgcct gcggtttaat cttttcactg tgagtgtcat atttcaggca
    24781 ctgacgagcg cgccactgtt caatttgctg ccaccagtcg cggatctcat ccagtggttg
    24841 atgggcggat tcttgcgaca agagttcaag catttgttcg aggacctggc gagcatcccc
    24901 cacaatcggg atatccgcag tcacggtttt agaaatggaa gtaggatcaa tatcgatatg
    24961 cagaacagtg gcatttgggc agtactttgc cagattgttc gtcgttcggt catcaaatcg
    25021 taccccgacg gcgaaaatca catccgcgtt atgcatcgtc atattggctt cgtaggtacc
    25081 gtgcattccc agcatgccca gtgcctgacg atgcgttgcc ggaaacgccc ccagccccat
    25141 caatgaggaa acaacgggca gattcaacgc ctccaccgtt tctttcaact gctgatggca
    25201 gcccgccatg attgccccac cgcctacgta gacaaccggt ttttttgccg ctaccagcgt
    25261 ttgcagagcg cgcttaattt gccctttatg tccggtagta gtgggattgt aagaacgcat
    25321 actgaccgac tcaggccaga catagggtaa tttgttcgcc ggattaagaa tatctttcgg
    25381 taaatcaacg accactggtc ctgggcgacc acttgccgcc agccagaaag cctttttcag
    25441 cacctgcgga atgtcttccg tttgcttaac cagaaaactg tgtttaacca ccggtcgcga
    25501 aatccccacc atgtcgcact cctgaaaggc atcgtaacct atcaacgagg tcgctacctg
    25561 cccggaaagg acaactaatg gaatggaatc catataagcg gtggcgatgc cggtaatcgc
    25621 attggtcgcc cctggacccg acgttaccag cacgacgccg acttccccgg tcgcgcgcgc
    25681 cagaccatcg gccatatgca ctgccgcctg ctcatgacga actaaaacat gatcgatacc
    25741 acccacggta tgcaatgcat cgtaaatatc aaggactgcg cctccgggat aaccgaatac
    25801 ttgtttaacg ccctgatcga taagcgatcg gacgaccatc tcggctccag acaacatctc
    25861 catggcctgc ctcactgttt gacggaaaaa atgtgtaaaa cacattaacc gtccgatctt
    25921 gcttaagcaa ttggcagcca gagacataaa cgcagggtaa taagaataaa caggaggaaa
    25981 ggtgaaaaga aacgataaaa tcctccatta cgaaggatta aattgataat aaatcggctg
    26041 aatcccacaa cttacagcaa tttctctatt tttttaatta atcctaaaac agagagccca
    26101 cacattcaga ataaaattct acattcaata aaaaatacag aataaaccag acattcatgc
    26161 ctgacctatt ctgcattcag ttagcgtttg caaattgaga ctaattgctc ttccatccac
    26221 tgatggcctt tatcgcgccc ggcagcttca tgccaggaga gataacaggt tctgctattt
    26281 tgttttaacg gcagcggtaa tacctgtaat tctaaggatt cagcgaactc ttcagccagc
    26341 caacgcggcg caatagcgac caaatgcgtt tgcgacacca cgctaagtac gctcatcatt
    26401 gccatgccct gatacgcgat actggcttgc ttatctaccg tgtcatacca aggttgacta
    26461 aatgacgcga aacgatcgag cgaaaccgcc gcatgttgtt cgttataaac atcatgtttc
    26521 agtaacgggc ccttaattgt tggatgattt ttgctggcta ccagcaccat ttcatcttta
    26581 aataatggca cgctggtaaa ttcaggacga tggaagtctt cataactaat cacaaactcc
    26641 gtttcctgat aacgcagctg atgttcagtg ttctgattta atgaagactt gaacataaca
    26701 tgtatatttg gcgcaatctg ctcaatgtga ttataaatct gcgaggtcag aatgctgtct
    26761 aacgggctgc aaacacaaag atgaaataca cgttcactgc tcgcgggttc aaaacctgaa
    26821 ccaggcaatt cattttgtac tagttgcaat gcctgacgaa ctgagccaaa aagttgaaat
    26881 gcgcgagcag tcggttgaat accacggcca taacgaacaa aaagctcgtc attaaacatc
    26941 accttcaggc gtgcaacagc gttactgacc gcaggttgcg acatacccag aacatgagcg
    27001 gcacgggtaa tgttttgctc ctgcatcacg gcatcgaaaa cggttaataa gttgagatcg
    27061 accatgcgta gctgtggctt gcttaactcc gccgtctctg aatgatctgt ttgtacctct
    27121 ggcatactta actccactgt cacacttaac tccctttccc ttattggaat gcagagaaat
    27181 aatcctgaag aatatgattt atcatgcata taaaataaga aaaagcaaaa tgagtaaaat
    27241 tcggaaaaca taaagatacg ggttctgata aaaacagaat ccattgccgt ggaatgagtc
    27301 atttaccaca gcataataat ccataatgtt tttgttgcga aaacaatcta atcatgacta
    27361 ccgcgaatac tcaatcatct acaaaatgta ttaaatgt
//
LOCUS       DAZGJW010000060        26729 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZGJW010000060 DAZGJW010000000
VERSION     DAZGJW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.060"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            118..1716
                     /gene="rtcR"
                     /locus_tag="ACHKIK_002486"
     CDS             118..1716
                     /gene="rtcR"
                     /locus_tag="ACHKIK_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO9083642.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETQVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYTRINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(1698..2456)
                     /locus_tag="ACHKIK_002487"
     CDS             complement(1698..2456)
                     /locus_tag="ACHKIK_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO9083643.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2473..3303)
                     /gene="glpG"
                     /locus_tag="ACHKIK_002488"
     CDS             complement(2473..3303)
                     /gene="glpG"
                     /locus_tag="ACHKIK_002488"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO9083644.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3348..3674)
                     /gene="glpE"
                     /locus_tag="ACHKIK_002489"
     CDS             complement(3348..3674)
                     /gene="glpE"
                     /locus_tag="ACHKIK_002489"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO9083645.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3864..5369
                     /gene="glpD"
                     /locus_tag="ACHKIK_002490"
     CDS             3864..5369
                     /gene="glpD"
                     /locus_tag="ACHKIK_002490"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO9083646.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5421..6026)
                     /locus_tag="ACHKIK_002491"
     CDS             complement(5421..6026)
                     /locus_tag="ACHKIK_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083647.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     AWGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6029..6796)
                     /locus_tag="ACHKIK_002492"
     CDS             complement(6029..6796)
                     /locus_tag="ACHKIK_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083648.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6799..7509)
                     /locus_tag="ACHKIK_002493"
     CDS             complement(6799..7509)
                     /locus_tag="ACHKIK_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083649.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANYKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCTRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7525..9033)
                     /locus_tag="ACHKIK_002494"
     CDS             complement(7525..9033)
                     /locus_tag="ACHKIK_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO9083650.1"
                     /translation="MMQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVC
                     NILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLIL
                     VGWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSL
                     GYGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLE
                     SSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMITRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(9159..11606)
                     /gene="glgP"
                     /locus_tag="ACHKIK_002495"
     CDS             complement(9159..11606)
                     /gene="glgP"
                     /locus_tag="ACHKIK_002495"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO9083651.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11625..13058)
                     /gene="glgA"
                     /locus_tag="ACHKIK_002496"
     CDS             complement(11625..13058)
                     /gene="glgA"
                     /locus_tag="ACHKIK_002496"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO9083652.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(13058..14353)
                     /gene="glgC"
                     /locus_tag="ACHKIK_002497"
     CDS             complement(13058..14353)
                     /gene="glgC"
                     /locus_tag="ACHKIK_002497"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO9083653.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(14371..16344)
                     /gene="glgX"
                     /locus_tag="ACHKIK_002498"
     CDS             complement(14371..16344)
                     /gene="glgX"
                     /locus_tag="ACHKIK_002498"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO9083654.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(16341..18527)
                     /gene="glgB"
                     /locus_tag="ACHKIK_002499"
     CDS             complement(16341..18527)
                     /gene="glgB"
                     /locus_tag="ACHKIK_002499"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO9083655.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18800..19903)
                     /gene="asd"
                     /locus_tag="ACHKIK_002500"
     CDS             complement(18800..19903)
                     /gene="asd"
                     /locus_tag="ACHKIK_002500"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO9083656.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            20095..20688
                     /gene="yhgN"
                     /locus_tag="ACHKIK_002501"
     CDS             20095..20688
                     /gene="yhgN"
                     /locus_tag="ACHKIK_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO9083657.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20745..22085)
                     /gene="gntU"
                     /locus_tag="ACHKIK_002502"
     CDS             complement(20745..22085)
                     /gene="gntU"
                     /locus_tag="ACHKIK_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO9083658.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(22089..22616)
                     /gene="gntK"
                     /locus_tag="ACHKIK_002503"
     CDS             complement(22089..22616)
                     /gene="gntK"
                     /locus_tag="ACHKIK_002503"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO9083659.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(22755..23750)
                     /gene="gntR"
                     /locus_tag="ACHKIK_002504"
     CDS             complement(22755..23750)
                     /gene="gntR"
                     /locus_tag="ACHKIK_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO9083660.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(23974..24669)
                     /gene="yhhW"
                     /locus_tag="ACHKIK_002505"
     CDS             complement(23974..24669)
                     /gene="yhhW"
                     /locus_tag="ACHKIK_002505"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO9083661.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(24792..25829)
                     /gene="yhhX"
                     /locus_tag="ACHKIK_002506"
     CDS             complement(24792..25829)
                     /gene="yhhX"
                     /locus_tag="ACHKIK_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO9083662.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADNSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            26162..26650
                     /gene="yhhY"
                     /locus_tag="ACHKIK_002507"
     CDS             26162..26650
                     /gene="yhhY"
                     /locus_tag="ACHKIK_002507"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO9083663.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         6667 a         7152 c         6660 g         6250 t
ORIGIN      
        1 attaattttt taattcattg tttttaaata ggttaaatct ttacgtccgt aaacggagat
       61 tttccacaaa accaattcac cgataatgaa atatcgtctt ttataaggat atctaagatg
      121 cgtaaaacag tggcttttgg ctttgtcggt accgtgctgg attatgccgg acgcggcagt
      181 cagcgctggt cgaaatggcg tccgacgctt tgtttatgcc agcaagaatc gttagtcatc
      241 gatcggctgg aattgttgca cgacacccgc tcgcgctcgc tattcgaaac gcttaaacgc
      301 gatatcgcca gcgtttcgcc agaaacacaa gtcgtgggcg ttgagattga actgcataac
      361 ccgtgggatt tcgaagaggt ttacgcctgc ctgcatgatt tcgcccgtgg ttacgagttt
      421 cagccagaaa aagaagacta tttaattcac atcaccaccg gcacccacgt cgcgcagatt
      481 tgctggtttc tgctggcgga agcgcgttac ctgcccgccc ggctgataca atcttcacct
      541 ccgcgcaaaa aagaacagcc gcgcggcgca ggtgaagtga cgattatcga tctcgattta
      601 agccgttata acgccatcgc cagccgcttt gccgaggaac gccagcaaac gcttgatttt
      661 cttaagtccg gcattgccac gcgtaacccc cacttcaacc gcatgattga gcagatcgaa
      721 aaagtggcga tcaaatcccg cgcgccgatt ctgcttaacg gtccaaccgg cgcgggcaag
      781 tcatttctgg cgcgacgcat cttcgagtta aaacaggcgc ggcatcagtt tagcggcgcg
      841 tttgtggaag tgaactgcgc caccctgcgc ggcgataccg ccatgtcgac gctgtttggt
      901 catgtaaaag gcgcgtttac cggggcgcgg gaatctcgtg aaggtttatt acgcagcgcc
      961 aacggcggaa tgttgtttct tgatgagatt ggcgaactgg gcgcagacga acaggcaatg
     1021 ctgctaaaag ccattgaaga gaaaaccttt tacccgtttg gcagcgatcg ccaggtgagc
     1081 agtgattttc agcttatcgc cggaacggtg cgcgatttgc gccagctggt tgccgaaggc
     1141 aaatttcgcg aagatctgta cacgcggatc aatctctgga ccttcaccct gccgggtcta
     1201 cgccagcgcc aggaagatat tgaaccgaac ctggattatg aagtggagcg ccacgcctca
     1261 ctcactggcg acagcgtgcg ttttaacacc gaagcgcggc gcgcctggtt ggcttttgcg
     1321 acctctcccc aggcaacatg gcgcggtaac tttcgcgaac tttctgccag cgtcacgcgg
     1381 atggccacct ttgccactag cggacgcatc actctggacg tggttgaaga tgagataaac
     1441 cgtctgcgct ataactggca ggagagccgc ccctccgcgc ttacggcgtt gctgggcgca
     1501 gaggcagaaa acatcgatct cttcgaccgt atgcaactgg aacacgttat cgctatctgc
     1561 cgccaggcaa agtcgctttc cgcagccgga cgccagcttt ttgacgtttc gcgccagggc
     1621 aaagccagcg tcaatgacgc ggatcggcta cgcaaatacc tggcgcgttt tggtctgacg
     1681 tgggaagccg tgcaggatca gcacagctcc agttgaatat ggtggtccgt cagcacctgc
     1741 atcacgctta ctggcggcgg ggcgtcggtg tagacggcat ctaccatgct gatgctgccc
     1801 atattgacca tcgcgttacg gccaaatttc gagtgatcga caaccagcat aacgtggcgc
     1861 gagttctcaa taatggcgcg tttggtgcga acttcgtggt aatcgaactc cagcagcgag
     1921 ccgtcgctat cgatgccgct tatccccaga atgccgaaat caaggcggaa ctgggagata
     1981 aaatcgagcg tcgcttcgcc aatgatcccg ccatcgcggc tgcgtaattc gccaccggcg
     2041 agaataatgc ggaaatcttc ttttaccatc aacgtgttag caacattgag attgttggtg
     2101 acaatacgca aattgctgtg attaagcagc gcgtgcgcca ccgcttccgg cgtggtgccg
     2161 atatcgataa acagtgtcga gccattgggg atttgctccg ccactttgcg ggcaatcctc
     2221 tctttttctt cggtctgggt ggctttacga tcgtgccacg gcgtgttaac cgaactggaa
     2281 ggcagcgccg caccgccatg gtgacgcagg atcaggtttt gctccgccag ctcattgagg
     2341 tcgcggcgaa tagtctgcgg gctgacggag aaatgctcta ccagctcttc agtactgaca
     2401 taaccctgct gtttaaccag ttcgataata ccgttgtgac gttgtgtttg tttcatttat
     2461 aaatccctgg aattattttc gttttcgcgc attgagcgaa tcaacaaaag ccatcgctaa
     2521 acccacggct aacccggcga tgtgtgctcc gttcgccatc gacatcccaa acaaatcaaa
     2581 ccatccggcg acaatccaga tcagcgcaaa gataattaac ccacgttgca ggtaaatgcc
     2641 actttgcgga tcgcgttcgc cacgtagcca gacgtagccc atcagcgcat acaccacgcc
     2701 agaaagcccg ccaaaccacg gcccgctgaa tttttgctgc acatagccgc ttaacagggc
     2761 gctaatgaga gtaataacaa ttagcttacc gctaccgagg cgtttttcca ccgcaccgcc
     2821 gagataccac caccagagca ggttaaagag gatatgcatc agcgagaagt gcattaacgc
     2881 gtgggtgaag taacgccaga actcaaattt cagtgttgga tcgaatggcc aggccagcca
     2941 taacatcact tcctgatcgc cgagaatttg catggcgata aacaccacca cgcaggcgat
     3001 catcaccacc caggttaccg gacctgcgcg ttcacgcaag gcagcaaaga aaggataacg
     3061 gcgataatgc agaccactgc cggtatggcc cgcctgccag ctggccgcca gataacgcgg
     3121 atctgccggg ttttcgagaa aacgcgccag ctccgcccgc acgcgctcgg cctgggactc
     3181 atccgccagc cagacatcgc tttggttatg ttgttgaatc gtgaggataa caccctgcgt
     3241 cgccatataa tcaacaaacg cctgcgccac gcgggggtta gcaaaagagg taatcatcaa
     3301 catcgttgct gtcgcttatt ccacacaaaa ggggacagta taaagcgtta cgcgccgtac
     3361 gccacctctg cgggaaactg acgctgccag gcttcaaaac cgccgtcaat gctatagacc
     3421 acatcgtagc cctgttgcag cagatactgc gccgcgcctt tgctgctatt gccgtgataa
     3481 cacatcacca tcaccggagt gtcaaagtcg ttatcacgca taaacgcgcc cagcgtgtcg
     3541 ttggttaaat ggaaagcctg caccgcatgc cccatagcga aactctgtgg atcgcgaata
     3601 tcgaccagca ccgcctcttt ttcctgcaac ttctggtgcg cgtcggcaac gttaatacat
     3661 tcgaacagat ccatgcgtct ctctttcttt acaaacaagt gggcaaattt accgcacagt
     3721 ttacgtcgaa gcggcagata aacgccataa tgttatacat atcactctaa aatgtttttt
     3781 caatgttacc taaagcgcga ttctttgcta atatgttcga taacgaacat ttataagctt
     3841 taacgaaagt gaatgagggc agcatggaaa ccaaagatct gattgtgata ggtggcggca
     3901 tcaatggtgc tggtatcgcg gcagacgccg ctggacgcgg tttatccgtg ctgatgctgg
     3961 aggcgcagga tctcgcttgt gcgacctctt ccgccagttc aaaactcatt cacggtggcc
     4021 tgcgctacct tgagcactat gaattccgcc tggtcagcga ggcgctggct gaacgtgaag
     4081 tgctgctgaa aatggctccg catatcgcct tcccgatgcg ttttcgcctg ccacatcgtc
     4141 cgcatctgcg cccggcgtgg atgattcgga ttggtctgtt tatgtacgat catctgggta
     4201 aacgcaccag cctgccggga tcaaccggtt tgcgttttgg cgcaaactca gtgttaaaac
     4261 cggaaattaa gcgcggattc gaatattctg actgttgggt agacgacgcc cgtctggtac
     4321 tcgccaacgc ccagatggtg gtgcgtaaag gcggcgaagt gcttactcgg actcgcgcca
     4381 cctctgctcg ccgcgaaaac ggcctgtgga ttgtggaagc ggaagatatc gataccggca
     4441 aaaaatatag ctggcaagcg cgcggcctgg ttaacgccac cggcccgtgg gtgaaacagt
     4501 tcttcgacga ggggatgcat ctgccttcgc cttatggcat tcgcctgatc aaaggcagcc
     4561 atattgtggt gccgcgtgtg catacccaga agcaggccta cattctgcaa aacgaagata
     4621 aacgtattgt gttcgtgatc ccgtggatgg acgaattttc catcatcggc accaccgacg
     4681 tagagtataa aggcgatccg aaagcggtga agattgaaga gagtgaaatc aactatctgc
     4741 tgaaagtgta taacgcgcac tttaaaaaac agcttagccg tgacgatatc gtctggacct
     4801 actccggtgt gcgtccgctg tgtgatgatg agtccgactc gccgcaggct attacccgtg
     4861 attacaccct tgatatccat gatgaaaacg gcaaagcacc gctgctgtcg gtattcggcg
     4921 gtaagctgac cacctaccga aaactggcag aacatgcgct ggaaaaactg acgccgtatt
     4981 atcagggtat tggcccggcg tggacgaaag agagcgtgct gccgggtggt gccattgaag
     5041 gcgaccgcga cgattacgca gctcgtctgc gccgtcgcta tccattcctg actgaatcgc
     5101 tggcgcgtca ttacgcacgc acttacggca gcaacagcga gctgctgctc ggcaatgcgg
     5161 gggcgataag cgacctcggg gaggatttcg gtcatgagtt ctacgaagcg gaactgaaat
     5221 acctggtgga tcacgaatgg gtccgccgca ccgacgatgc cctttggcgt cgcacaaaac
     5281 aaggcatgtg gctgaatgcg gatcaacaat ctcgtgtgag tcagtggctg gtggaatata
     5341 cgcagcagaa gttgtcgctg gcgtcgtaag gatgatgccc ggttgaagtg caaccgggct
     5401 atttggattc cttagactat tcattgtaaa taattgaata acggcccctg agctaaaatc
     5461 gccaaactta agcacgttac ccaatcgccc cacttcttcc tctggtttct attttttctg
     5521 caccaatggc aataatatcc ccatgcaaaa ccaagctgtg cactaatcgg gccgacaaaa
     5581 tcagtccaag gctgcggcca aaaaggggaa aaattcaggg gaggccagac aaacgtcaga
     5641 aagaaatttc ggatattttc cagcaacgct gatttctctg gagaaatata aagaatgaaa
     5701 ttcgagagaa ttaaccagaa atagaccggc gaaaagacca gccagcaatg cagtctgttt
     5761 tttaattcag gaggcgttct gcgggagaat tgccaaccga tgccagccat tacactgaaa
     5821 aaaagtgcat attcaaccat taactcaagt tgtaattcct gaagagaaat acgcacaacc
     5881 aggagcggaa aggagaatat tatttctgcc agaaaagtta acgtgaatat aagtacggct
     5941 aagacaataa ccgtcggcca taccaataat ggcacgataa caaaaccgag aattacaggg
     6001 ggaatataaa gccggaattt aatcattttt attttcctgg tgattcggat gatgcgtcat
     6061 acatcgcgca taaacactca tcaattatcc agtcatcttc ccagacctga tattctgtca
     6121 ttggttcggg ctgttttagc gggttctcaa tcgcttgccg cgctctttct ttcagtaaag
     6181 gatgattatt tctagcccag gcattgattt gggtggtaat ggcgttatcg atatcgtcct
     6241 ggttatctct gatataatcc tcacaatatc tgttcaataa acaggcaaga gaaacggtcg
     6301 accaaaagcc gggaactgaa ttaatggcat ccagcaatgg cgcaagagcg atatcggcac
     6361 ggaaaaacat ttccagcata tactgtaatt cgacagcttc atcgccggat acccaatcag
     6421 tcatatactg gcaggcaaat cgctgtaaaa ttgcccgttc atccgcacgc cagttttctt
     6481 tgttggcaag gttgatacgg cgcagagaaa aaactacgtg aatattgtca aaatgcccct
     6541 gtgcaacaaa ttccagtaaa cgcggcagaa aatagcggac ctcatcgcta ttttgcggat
     6601 caggccctgg gctactattc caggcattga ttaaggaata cgggatcgct cgtaacgatg
     6661 tactccgtaa tgccttttgt tcctgctgac tcaggcaata ttcacaacaa accacaaatt
     6721 tctgcggcac ccgataacga cgaaaggtgt gataaatctg ctcaattaat gcctgcatgg
     6781 cggggctggg ttgcatagtt accccctcgc ccgctgatcg cgtgtaaacg gttgatagac
     6841 aaaacgagca ttcgccgcgt tcaggcgaat aagggcgcta cgcgaaaggg aaaagctggg
     6901 gcgagtgcaa ctattgcagt ctcgacagct ttcctctgcg caattttcca ctgagtaatg
     6961 acgcgtgtag agtttcagtt cggcctgctt atcgcgaaat ccgcgtaaac tgttattgtt
     7021 atgaaatttc caggcatctt taaagacctg aaagatatag acatcctcaa tccaggagtc
     7081 acatttttta agcgggcgct ttgctattat cacccgctca tccggattta gcatcgccgt
     7141 aaattgtacg gtgacgtcat ttgtcgtgtt caggtcacat tgaacatctg gtacatcact
     7201 gatatagggg tcaaatccaa aatcagcacc acaggcacaa agacggctaa tggtggcggt
     7261 atcgagggaa aatcccggcc ataccttgct ggaggttaag ccaatattta atgtgacatc
     7321 atatcgaagc aatccctcaa gaccgtcagc accgacgaac ttttcccctt catccagggc
     7381 ctggttaata agttgctgaa caatgccttc atcccagtat gccgatttgt agttggcaat
     7441 cgtaatatcc acgcctgtga agggatcagt accggcaagg cttgcaaata atgtattaac
     7501 taattccatt tttattatcc agctttaagg tagcgtctta tacatcggga cataacctac
     7561 atcttccacc aaactctgtc cctgcggcgt taaaaaccac tccaacaatt tttgtgtctc
     7621 tgacgtcgta ttttcccgcg ttatcataaa ggcatcgacg atatagggat atttgccgtt
     7681 gcgaatgttt tcggcagtcg gtgcaatacc gttaatcgct agcagtttta tatttttatc
     7741 ggcgttcatt tgtgttgcgt aataacggaa ggtgtaacct attgcgttgt ttgtattacg
     7801 gtattcggca acgactttaa tcattccctc catcatactt gccacttctg tttcctgcgg
     7861 cgagatcatg cggacatttt tcatgacctg ggattgcatc accgtctggc taccagagtc
     7921 ttccgggcgt tgccaggtct ggatctcctg atcgttaccg ccaacagtac gccagttggt
     7981 aattgcacca ctgaagatgt cacgcacctg ttgttcagtc agggaattaa ccggattatc
     8041 cgcattgacg atgaaaacaa acgcttcacg ggcaaaaggc gtgtacagca aagtaacgcc
     8101 cgattcctca gcgcgttttt tctgcccacc ggaaggttgc gccacaaaaa taatatcagc
     8161 atcaccttta acaatcctgt tatacgcatc gggggtacga gagctctcca gatattccct
     8221 ggtgtgaaaa tcttccggta taacgcttaa agcataaaat gcagaagcat aaattgggta
     8281 cgccgccgta gcaccatcga gtcgcggcca gttttgcgta aactgaattt gcggtttacc
     8341 acgcagtggt gtcagttgat tattgagttt atcgggtcgc caggcccagg tatcaatatt
     8401 ttcgcgcaca ccgacgccgg gattgagctg tttatataaa taaccctgat aaccagcgac
     8461 aaaaccaagc atgagaagga taaacaggag ggaattacgg caacgtagcc cggatgtacc
     8521 ggttaaagga tgtctggttg gccggccgta ccccagcgaa aaactgatat gcgcgccaac
     8581 gggtataatg actatccacc agtactcacc tccgactact gagactaaca aaacgcccag
     8641 ccaggggagc ataagatcag ccaaccaacc cgatcccatc tggtgaaatt gataatctgc
     8701 cagatgcagc gcgattatcc agccaaccag gattaataaa ccgggtacta aaaaaagaca
     8761 ttgtcgccag acggctattt ctcttttttc tggtgcacta tcgcctgccc atactcgccc
     8821 cgagaaaaat agcattaatg cgcccagcgc cagcataaaa atgttgagca gaaggtaact
     8881 tgttggcgtg ataaggatat tacaaacttc aataataccg gcaagtccga cacccagcat
     8941 agcaacaacc gccgccaaaa attgcgtcag tatggggatg ccgaaaaaag tcattaccag
     9001 cgaggacaaa atccattttc tgttttgcat tattctttcc attctttttg aatggtgaaa
     9061 ttatactccc cgagtcccct tgccccttct ggacactttt ccgaaatgat ggcggaaaaa
     9121 aacgggagcc tttggccccg ttctatttat tggtgaactt acaatctcac cggatcgata
     9181 tgccagatat gatcggcgta ctctttgata gtacggtcag aagagaagta gcccatattg
     9241 gcaatgttca gcatcgcttt tgcggtccac tcttcctgaa gctcgtagag ttcgtcgact
     9301 ttatcctgac aatcgacata gctgcgataa tccgccagaa cctgatagtg atcgccgaag
     9361 ttgatcagcg aatcaaccag atcgcgatag cgacccggat cttccggact gaatacaccg
     9421 ctgccgattt gcgtcagcac ctgatgcagc tcctcatctt tctcgtagta ttcacgcggt
     9481 ttgtagccct gacgacgcag ttcttccact tcttccgcag tgttaccaaa gataaagata
     9541 ttgtcagcac cgacatgatc cagcatctcg acgttcgcac cgtccagcgt accgatagtc
     9601 agcgcgccgt taagcgcaaa cttcatgtta ctggtgccgg aagcttccgt ccctgccagc
     9661 gaaatctgtt cagacagatc tgccgccgga atgatcaact gcgccaggct aacgctgtag
     9721 ttcgggatga acacgacttt cagcttatcg ccaatctgcg gatcgttgtt gatcactttc
     9781 gctacgtcat tgatcaaatg aataatgtgc ttcgccatgt aataggccga agccgcctta
     9841 ccgccaaaaa tattcacgcg cggtacccac ttcgcatccg ggtcggcctt gatgcggtta
     9901 tagcgggtaa tcacatgcaa cacattcatc aattgacgtt tgtattcgtg aatacgtttg
     9961 atttgtacat cgaacaacgc ctttggattc accaccacat tcagctgctg ggcgatatac
    10021 tctgccagac gctttttgtt ctccagcttc gcctgatgca cagcgtgatt aaccattggg
    10081 aaatcacagt gttgttgcag ctcattaagc aggctaaggt cggtgcgcca gttacggccc
    10141 aggtgttcgt ccagcacggc tgaaagcgat gggttcgcta ccgctagcca gcgacgcggc
    10201 gtcacaccgt tggtgacgtt ggtgaaacga cccgggaaga ttttcgcaaa gtcggcaaac
    10261 aacgattgca ccatcagatt agagtgcagt tccgatacac cgttaacttt gtggctcaca
    10321 acaaccgcca gccaggccat acgcacacga cgaccgttgg attcatcaat gatcgacgcc
    10381 cgtcccagca gatcggtatc gttcggatac tgttcctgca aggttttcag gaaatagtcg
    10441 ttgatttcaa agatgatctg caggtgacgc ggcagaattt tacccagcat atcaaccggc
    10501 caggtttcca gcgcctcgct catcagcgta tggttagtgt aggagaagac ctgacaacac
    10561 acctcaaacg cgtcgtccca gctaaattgg tgctcatcga tcagcagacg catcatctca
    10621 ggaatcgaca gtaccggatg ggtatcattg agatgaatcg cgattttatc cgccaggtta
    10681 tcgtaggttt tatgcaactg ataatggcgg cttaaaatgt cctgaatggt cgaggaaacc
    10741 aggaagtatt cctgacgcag gcgcagctca cgcccggagt aggtggagtc atccggatac
    10801 agtacgcgag atacgttctc ggagtggttt ttatcttcca ctgccgcgaa gtagtcaccc
    10861 tggttgaatt taccgaggtt aatttcgcta ctggcttgcg cactccacaa acgcaacgtg
    10921 ttggtcgcgt cggtgtcgta accagggatt atctgatcgt aagcgactcc cagaatctct
    10981 tcggtttcaa tccagcgcgt ttttttacct tcctgctgaa tgcgaccacc aaaacggact
    11041 ttatagcgcg tgttgtggcg tttgaattcc cacgggttac cgtattccag ccagtagtct
    11101 ggcgactctt tctggctacc gttaacgatg ttctgcttga acataccgta gtcatagcgg
    11161 atgccgtaac cgcgccccgg caaccctaac gtcgccagag aatcaaggaa gcaagccgcc
    11221 agacgtccca ggccaccgtt accgaggcct gggtcatttt cttcatcaat cagctcttcg
    11281 agatttaacc ccatcgcttc cagtgcgccc tgtacatctt cgtaaattcc tagcgacaac
    11341 atggcgttgg agagcgtacg gccaatcaaa aactccatcg acaggtagta aacctgacga
    11401 gtttcttgcg acaactgggc acggtttgaa cgtaaccagc gctccacgag acgatcgcgc
    11461 acagcaaata acgttgcgtt cagccattca tgtttattgg cgacgaccgg gtcctttcca
    11521 atcgtaaaca tcagcttgta agcgatagag tgcttaagag cttctacgct aagcgtgggc
    11581 gatgaatatg taaacggagc attcatatag gcgtttcctg aaaactattt caagcgatag
    11641 taaagctcac ggtacgactt cgccgcgacc tgccagctaa aatccattgc catagcctga
    11701 cgttgcacaa accgccacag tgaaggacgg gaccacagta caaaagcacg tcgaatagcc
    11761 cgtaacagcg accaggcatt actatcttca aagacaaacc cactggcgac gccatctgca
    11821 aggttctcaa gagaacagtc agaaaccgta tcagcaagcc caccggtgcg ccgcactaac
    11881 ggcagcgtac cgtacttcaa tccataaagt tgcgttaagc cgcacggttc aaaacggctg
    11941 ggcaccagaa tgacgtccgc gccgcccata atgcgatgcg aaaatgcttc gtgatagcca
    12001 atctgaacgc ccacctggcc ggggtattcc gctgccgccg caaggaaacc ttcctgcagc
    12061 accggatcgc ccgcgccgag tagcgccagc tgcccgccct gctccagaag gcccggtaag
    12121 gcttccagca ccaggtcgag acctttctgg ctggtcagac ggctcaccac tgcaaaaagc
    12181 ggcactttat catcaacctt aagccccatt gcgatttgta actggcgctt attttccgct
    12241 ttatcttcca acgtatcgcg ggtgtagcgc gaggccaaca gtaagtccgt ctctggactc
    12301 cagattttct cgtccacgcc gttcagtacg ccggaaagac gcccttcacg gtgacgctgt
    12361 tgcaacagac cttccatacc gtaggcaaac tgcggttcgg tgatctcgcg agcgtaggtt
    12421 ggactaaccg ccgtaatgtg atcggcatag tacagaccgg ccttcaggaa agagatttgt
    12481 ccgttgaatt ccagcccatg aatattaaag aatgaccatg gcaattggat gtcattcata
    12541 tgatgtgcat aaaacatgcc ttgataggcc aggttgtgca cagtaaacac cgacttcgcc
    12601 ggacgcccgc gcgccgccag atacgcaggc gcaaggcctg catgccagtc gtgcgcatgc
    12661 accacatcag gacgccagaa tgggtcaagc ccgctggcca tttctgcccc aacccacccc
    12721 agcagcgcaa aacgcaatac gttgtcggta taggcaaata agttggtatc gtgatacggg
    12781 cttcccggac gatcatagag atgcggcgcg tcaatcaggt atatgccaac cccgttgtaa
    12841 tgaccgaaca acagcgtgat atgtccggcg aaggtatcac gacgggatac tacctgcgca
    12901 tcggtaacgc cacggcgaat atcgggaaat gcaggcaaca gtacgcgagc gtcaacgccg
    12961 tctgcgattt gtgctgcggg taatgcccca ataacatcag ccagaccgcc ggttttaagc
    13021 agcgggaaca tctctgaaca tacatgtaaa acctgcatta tcgctcctgt ttatgcccta
    13081 acttccgtag catttcgcgc gttaccagca cgatgccttc ttctgaacga tagaaacgac
    13141 gtgcatcttc ctctgcgttt tcaccaatca ccatgccttc cggaataaca caagcacgat
    13201 cgatgacgca gcggcgcaga cggcacgagc gacctaccca tacttccggt aacaatacgg
    13261 cggaatcaat gttgcagaat gaattcacgc gaacgcgcga gaacagaacg gactgcacca
    13321 ccaccgaacc ggagatcaca cagccgccgg ataccagtga gttaagggtc atcccgtggc
    13381 taccggagcg atcctgcacg aatttcgctg gcggtaatga ttcattgtag gtgcgaattg
    13441 gccaattgcg atcgtacata tccagttccg gcaccacaga ggccagatcg aggttcgctt
    13501 tccagtaagc ttccagcgta cccacatcgc gccagtacgg ctcggcatcc gggtcggatt
    13561 gtacgcaaga gagcgggaac gggtgcgcat aggccagacc ggcttcggtg atcttgggaa
    13621 tcaaatcttt gccaaagtcg tggctggagt tctcatcacg atcgtcttct tccagcagtt
    13681 catacagata gtcggcgtca aagacgtaga tacccatact cgccagagat ttgctcggat
    13741 cgttcggcat tgacggtggg ttagcaggtt tttcaacgaa ttcgataatt ttatcgttct
    13801 catcaaccgc cataacgcca aatgcggagg cttcttcaat cggtactggc atacaagcaa
    13861 cggtgcaacg tgcgcctttt tcgacgtgat cgataagcat acgcgagtag tcttgcttgt
    13921 agatatggtc gcccgccagg atcaccacgt attccgcttt ataacggcgg ataatgtcga
    13981 ggttttgggt gaccgcatct gcggtgccgc gataccagtt ttcccctttc attctctgct
    14041 gtgctggcag cagatcgaca aactcgttca tttcttcatt gaagaatgac cagccgcgct
    14101 gaatgtgctg caccagagtg tgggactggt actgggtgat cacgcccata cgacggatcc
    14161 cggagttgat gcagttagac agcgcaaagt cgataatgcg gaacttaccg ccgaagtgta
    14221 cggccggttt tgctcgctta ttggttaaat ccttcaggcg ggtaccacgt cctcccgcca
    14281 gtatcagggc aacagatttc aatggcagct ggcgcgccaa cattaagtga tcgttcttct
    14341 ctaaactaac catgactaac tcctttttta tcatctctgg aacacacaca atccgtgtgc
    14401 aggtccctgc cagacagccg taatcactgg gttatcctct ccagcgaatg ggggaatggc
    14461 gtgccactcc ccagcaggta aaacaatctc tgttacctca agcgtggcgt taattgcgat
    14521 caaaaagcga tccgagagca gaatttgcag ctgtttcggc ccgttttgcc actcatccgt
    14581 gcttaaaggt tgagcatatc gatttagcca acggacattg ccgtcgcctt cttcccacca
    14641 gcgattctcc accaaagcgg gaatgcgctt gcgcagatgg attaacgcgg cggtaaatgc
    14701 ggttaaacca ctgcttgcct gcgaccagtc caaccaggtt aattggttat cctgacagta
    14761 ggcattgtta ttgccatgct ggctgtgacc atgttcgtca ccggccagta acatcggcgt
    14821 accctgggag agcaacaacg ttgttaacag ggcgtgaatg ctgtcgcgcc gccgttcaac
    14881 caggtcaaga gaaccgccta acccttcttt accatgattg ttactgtaat tgttgttggt
    14941 cccgtcgcga ttttcttctc cgtttgcttc attgtgttta tggttgaagc aaacgcagtc
    15001 gcgaagcgta aaaccgtcat gcgcggtgac gagattaatc gcggcactcg gcagacgacc
    15061 attacgttta aaaacatcgc tggaggcagc aaaacgcccg gcaaacgccc ccagaggcaa
    15121 atcataatgt agccagaaac gacgggcagc atcgcggaaa tgatcgttcc actcggcaaa
    15181 cagcggcggg aaatttccca cctgataacc accaggagcg atatcccacg gttcagcaat
    15241 taacttcacc tgcgagagca ccgggcagtt ctggatagcg gtaaacaacg gcgcatcctg
    15301 acggaactct ggcgtacggc ccatgactgc cgccagatca aagcggaaac catcgacgtg
    15361 gcaggtttct acccaataac gcaggcaggc gctggcataa tccaccaccg ccggatgact
    15421 caaattgagc gtgttgccgc aaccggtcca gttgtgataa tcgccgtctt ctcttatcca
    15481 ataatagcta cggttatcga tcccacgcag cgagaataac gggccgtcga ggtccagttc
    15541 cgcactatgg ttgagcacga tatcaagaat gacttcgata cccgctttat gcagtgcttt
    15601 gattgcatcg cgaaactcat ccagcgccgt ttctggcgag caggcatacg ccggatgcag
    15661 cgcaaacatc gccaccgggt tgtaacccca gtagttactt agccccatgc gttgcagacg
    15721 tggttcactg gcaaactgcg ccactggcag cagttccagc gcggtaatgc ccaattgttt
    15781 caaatagttg atcatcaccg gatgcccgag ggctttataa gtgccacgga tctcgaccgg
    15841 gatctccggg tgcaagtacg ttaatccttt gacatgggct tcataaatga tggtgctgcc
    15901 ccacggcgtg cgcggcgggg catcatcttc ccagtcatag tgatcaacca ccactacgca
    15961 tttcggcgca atggcggcgt tgtcgcgata gtcaggttca ttatgaccgg cgtgcagcag
    16021 cgggttatct ttaaactccc cgtcaatttg ccgcgcgcaa ggatcaatca acaacttcgc
    16081 cgggttaaag cgatgcccct cggcgggttg ccaggggcca tgaacgcgat aaccataacg
    16141 caaacccggg cgcgcatccg gcagataacc gtgccaaatg tcgccactgt gccctggcaa
    16201 gtcatagcga tgttcctggc cattggcgtc aaagacacac agttctaccc gctcggcatg
    16261 agcggagaaa agtgtgaagt tgacgccctg accgtcgtaa tgcgcgccga ggggagcggg
    16321 tttgccaatg gcgagttgtg tcattctgcc tcccgaacca gccagatagt ggccagcggt
    16381 ggtagcgtca ggcttagtga atgctgacga ccgtggctgg caatctcatc gctgtgtacc
    16441 gtgccgccat tgcctgcatt actgccgtga tagtgcatgg aatcggtatt gaggatttca
    16501 cgccatttgc ccggctggtt tatgccgaag cgataatcat gacgcggtac cggcgtaaag
    16561 ttactggcaa cgatgatttc gttaccctct ttatcgcgac gcacaaagat cagcaccgag
    16621 cgttctttgt catccaccac cagccattca aagccgtacg ggtcaaaatc cagttcatgc
    16681 attgctttat ggtggcggta ggtgaggttc agatcgcgca ccagacgctg gacaccgtgg
    16741 tgccagttat cgccgccttc caacagatgc cagtcgaggc tggcgtcatg gttccactcg
    16801 cggccctggg caaattcgtt acccatgaac agtagtttct tgcccgggaa tgcccacatc
    16861 cagccatagt aggcgcgcag gttcgcgaat ttctgccatg cgtcgcccgg catgcggtcg
    16921 agaatcgatt ttttaccgtg gaccacttca tcatgcgaca acggcaggac gaagttttca
    16981 gtgtagttgt agagaatccc gaaggtcagt ttatcgtgat gatactgacg ataaaccggg
    17041 tcgagcttca tgtagtccag ggtgtcatgc atccagccga ggttccactt gtaccagaag
    17101 cccagaccgc ccatatcctg cggacgagaa acgccaggga aatcggtaga ctcctcagcc
    17161 attgtcaccg caccggaaac ctgctcacca agaatacggt tggtattacg caagaattca
    17221 atcgcttcaa gattctcgcg cccgccaaat tcgttcggga tccactcccc ctctttacgg
    17281 ctgtagtcgc gataaatcat tgacgccacc gcatcgacgc gcagcgcatc aataccaaaa
    17341 cgttcaatcc agtaaagcgc gttaccgacg aggaagttac tgacttcacg gcgaccatag
    17401 ttgtagatca gcgtgttcca gtcctgatga tagccttcac gcggatcgct gtgttcatac
    17461 aagttcgtgc catcaaattc ggcaagcgca aagtcatcag tcgggaagtg gcctggcacc
    17521 cagtcgagaa tcacgttcag accagctgcg tgtgcggcat caatgaaata acggaagtcg
    17581 tcgcgagtac caaaacggcg ggttggtgca tacaggccgg ttggctgata accccaactg
    17641 ccatcgaagg gatgctcgtt aatgggcagt agttcgaggt gggtaaagcc catccattta
    17701 gcataaggca ccagttgatc ggccagctcg cggtagctca accagaaatt gttgtcggtg
    17761 tggcgacgcc aggaacccag gtgaacttca taaatagaga ttggcgcatc aaactgattc
    17821 gcttttttgc gctcttcagt ctgtacaacc ttttccggca gcccgcaaat aagagacgcg
    17881 gtttccgggc gcatttgcgc ttcgaaggca taagggtcgg acttcagacg caagttgcca
    17941 ttggcatcaa tcatctcgta tttatagagc tgaccgttat gcgccccagg gataaacagt
    18001 tcccagatgc cgctctcttt acgcaggcgc atcgggtgac ggcgaccgtc ccagtagttg
    18061 aattgcccaa ccaccgagac ccgacgggcg tttggagccc agacagagaa acgcgtacct
    18121 gtgacgccat ccatagtatc tgcatgcgcg cctaaggttt catacgggcg caggtgagta
    18181 ccttcagata atagccaggc atccatttcc tggattagcg gaccaaaacg gtaaggatca
    18241 tcaatcaggt tttgctgacc atgccagaca acagccaact gatagcggaa aaaattctta
    18301 cgtcgcggaa tgacgccgct aaagaatccc cgtgagtcga gacactccag ttttgcgagt
    18361 ttgcgcccgg ttttcggttc aatcacccac acatcggtag cgtcgggtaa aagggcacgg
    18421 acttccagtc ccgcggtggt tttatgcatt cccagtacgg aaaaaggatc cgcaaaatgg
    18481 cctgcaatta gcgcgttaat cacgtctcta tcgatacgat cggacatgct tgtcttcctg
    18541 ttttattgtg tcaccccatc caattggatt tttgacttct ggttgtgaca tttttttgac
    18601 ctgaacggcg cagcactctg tgcatcctct ctgcgtcgtc ctcacttcag gtaaggctgt
    18661 gaatactcat gtattcagcc acccttaaag aatagccaat gctctattta actcccggta
    18721 aatcatgaaa catctgcgct tactcctgta ttacgcacta acaggggcgg catcgcgccc
    18781 cagatttaat gaataaagat tacgccagtt gacgaagcat ccgacgcagc ggctccgcgg
    18841 ccccccacag cagctggtcg cccacggtaa aggctgacag gaactctggt cccatattca
    18901 gcttacgcag gcggcctacc ggcgtggtca gcgtgccggt aacggcagct ggggttagct
    18961 cacgcatagt gatttcccga tcgttcggaa cgactttcgc ccacggattg tgcgcagcca
    19021 gcagttcttc cacggtcgga atagacacat cttttttcaa tttaatagtg aatgcctggc
    19081 tgtggcagcg caatgccccg acacgcacac ataaaccatc taccggaatt acggaagatg
    19141 tgttgaggat cttgttggtt tccgcctgcc ctttccactc ttcgcggctc tgaccgttat
    19201 cgagctgttt gtcgatccac ggaatcaggc tacccgccag cggcacgcca aagttatcca
    19261 ccggcagctc accgctacgg gttaaggttg tgactttgcg ttcgatatcg agaatagcag
    19321 aggacggggt cgcgagttca tctgccacat ggccatacag atggcccatc tgggttaata
    19381 actcacgcat atgtcgcgca ccaccgccgg aagcggcctg gtaggttgca acggacaccc
    19441 aatcaacaag atcattggcg aataaaccac ccaacgacat caacatcagg cttacggtac
    19501 agttaccgcc aacaaaagtc ctgatgccat tatttaatcc gtcggtaatg acgtcctgat
    19561 tgacggggtc aagaatgatg atggcgtcat ctttcatgcg cagagacgat gctgcgtcaa
    19621 tccagtaacc ttgccatccg ctttcacgaa gctttggata gatttcgttg gtataatcgc
    19681 cgccctgaca ggtcacaatg atatcgaggg cctttagcgc ctccagatca aaggcatcct
    19741 gaagtgtgcc agtggttccg ccaaaagacg gcgcagcctg gccaagctga gaagtagaaa
    19801 agaagacagg gcgaatggcg tcgaagtcgc gctcttcaac catgcgttgc atgagaacgg
    19861 agccgaccat accgcgccag ccgataaaac caacattttt cataagcgtt ttttcctgca
    19921 aagatgtgtg ctgtataaat gtgccggtct cctcttggca catctttcac catacaaaaa
    19981 gcagccaaag tcgcaagtga aattaatcaa tgatagcgaa gccatcagta atgcgactta
    20041 tcctgctttg ttagcacgca gaaagtccgc ggcaattatc agggaatttg agttatgaat
    20101 gaaatcattt ctgcagcagt tttattgatc ctgattatgg atccgctcgg aaacctacct
    20161 attttcatgt ccgtactgaa acatactgaa ccgaaaagac ggcgggcaat catggtgcga
    20221 gagttgctta ttgctctcct ggtgatgctg gtgttcctgt ttgcgggtga gaaaattctg
    20281 gcatttctca gcctgcgagc agaaaccgtc tccatttctg gcggcatcat tctgtttcta
    20341 atcgccatta agatgatttt ccccagcgct tcaggaaata gcagcgggct tccggcaggt
    20401 gaagagccat ttatcgtgcc gttggctatt ccgttagtcg ccgggccgac tattctcgcc
    20461 acgctgatgt tgctgtctca ccagtaccca aatcagatgg gacatctggt gattgctctg
    20521 ctgctggcct ggggcggcac ctttgtcatc ctgctacagt cttcgctatt tttacgtctg
    20581 ctgggcgaga aaggggtgaa cgcacttgaa cgcctgatgg gattgattct ggtgatgatg
    20641 gcaacccaga tgttcctcga cggcattcga atgtggatga aggggtaacg aggagagttt
    20701 ttcgaaaaga acccggtagc gtcacaacta ccgggcgaac aaacttaact caacagctgg
    20761 aacgcaatca tcccaacgat ggcaccgaca gtgccgagga tggtttccat catggtccag
    20821 gttttcagcg tttcggcttc ggtcgcgccg gtaaatttac cgaacaacca gaaaccggcg
    20881 tcgttaacgt ggctgacaac aatcgaacca ccagcgatac aaatcgacag cgccgccatt
    20941 tgcgcaccgg agtagttcag ttgttcaata accggcatca ccagtcctac cgccgttaaa
    21001 caggctacgg tggcagaacc ctgaatgatg cgcactgcag cggccagcac gaagcaggtg
    21061 atggctatcg gcaggcccat gccagttaac gcttcgccca gtgccggacc tacgccagag
    21121 tcaaccagca cctgtttgaa cacgccacct gcaccaatca ccagcagaat gatccccgcc
    21181 ggttgcagcg cgtgaccgca aatctccatc actttgtctt ttggcatgcc ctgacgcatt
    21241 gccaggccat aaatcgctac cagacaagca accagaatcg cggtaaacgg atgaccaata
    21301 aactcgaacc attcgtaagc ggtagagcct tctggcacaa aacgcgcggc aatggttttc
    21361 agccccacca gcaccagcgg caacaggatc agcgacaggc tgaatccgaa agatggcatt
    21421 ttgccttcgc cgagatgcgg ttcgctgatg tcgtcaggaa tatgcaactc cacgtagcgg
    21481 ctgatgaaat taccccacag cggcccggca ataatcattc ccggaattgc cgcacacagg
    21541 ccaatcagga tcatccagcc aaaatcggcg ttcatctgcg atgccagcag cattggcgct
    21601 ggccctggca ccaggaacgc cgccgctgcc gccacgcctg cgaataatgg gattaccagc
    21661 ttcaccaggt tcgtaccggt gtggcgcgcc attgagaaag caacgctaat cagcagaaca
    21721 atcgccactt caaagaacag cggtagcgca cagaccagcc ccgcaaggcc gatggcataa
    21781 tgcgcgcggc tgtgaccgaa ggatttaagc attttgacgg caatctgatc gactgcgccg
    21841 gtttcatgta agatcttgcc aaacatagct cccagggcga caaccaccgc caggaagccg
    21901 agggtgcctc ccatcccttt ttccatcgtc gctgcgattt tatcgagcgg cataccggaa
    21961 aaaaggccag cccccatgga caccaccatc aaagccagga aagcgtgcat acgcgccttc
    22021 atgactaaaa acagcagcag taaaacagac cctactgctg ttaaaacaag cgttaatgta
    22081 gtcactactt atttgccttt tttaataacc tcaatggtgc ttgccacaac accttccagc
    22141 ggttgatcga tatccaccac cagtacatca gtttcgtccg cacccggctc ctgcagcgtt
    22201 tcaaactgcg tcaccaacat ttgggtttta aagaaatggc ctttgcgcgc tttcaggcgg
    22261 ctttcaatca cgtcaaaatc gcctttcagg tagatgaaag agagattcgg attaccttca
    22321 cgcagcaagt cgcgatagtg ttttttcaat gcagaacaga cgatcagtga cactttatta
    22381 gtgcgctgca tagcaaacgc ggcgtcgttc agcgcctgca accacggttt gcgatcgtcg
    22441 tcattcagcg gttcgccaga cgccattttt tcgatattgc gccgtggatg gaggaaatcg
    22501 ccatcaagaa acgcggcatg aagttgatgc gccacttcac tggcgaccgc agatttgccg
    22561 ctgcccgata cgcccatcaa gacgtaaatg tggtgatcat ggttagtcgt gctcaaagtg
    22621 gtgcccccac aatacaagaa ttaaaaatgt tacgggtaac tgttatcggt aacattgtcc
    22681 agccggacaa tatcagaagc aatatccatc cgtgccttaa gtgtataagt gtgagctact
    22741 tcaaatttgt aggcttaaat agatccgccc ggtgacaagg tgaaacctaa atctaacatt
    22801 ttcggtgtca cagattcgcc acgaatacgc gccagcaggc gttcagcgcc aatactgccc
    22861 atccgctcac gcggcgtcag cacgctcgct aaccgcggct ccatcacctg gccaatgtca
    22921 tgaccgtgga aaccggcaat cgccatatcg tcaggaacct tcaatcccag acgctgacat
    22981 tcaaacgccg cgccgaccgc caggtcatcg ttggtacaga acacgccatc cagctgcgga
    23041 tattcccgcc gcgcctggcg aatcagttca ataccggaag agtaagaaga agattgctca
    23101 accatcacgc tatacggcac cagacctgca tccagcatcg cctgttcgta tcccttctgt
    23161 ttgatgatag tacgttcgtc gagacgcgcg ccgagatagg caatgtggca atgcccgcga
    23221 gcaataatgg cggtggtcat ctggcgtgcc gcttcaaagt tatcaaaacc gacggcgata
    23281 tcgaggcacg gcgactggct gtccatgagt tccaccaccg ggatccccgc cacttcaatc
    23341 atctttaagg tgcgcggcgt gtgggtacgt tcggtgagga tcagtccgtc gatattccat
    23401 gagagcatgg attcgagacg ttcttgctcc atttccggtt tataaccgta gtgcgccagc
    23461 atggtctgat aaccgtgcgc gtcggtgacg ctttcgattc cgcgtaatac ttccgcgaaa
    23521 acctggttgg tgagagaagg taacaggacg ccaatcgccc ggctggtggc gttagagagg
    23581 atatcgggcg cacgattggg aatatagccc agttcatcaa gagcggcggc aatcttgccg
    23641 cgtagagcga cggaaacctg ctccgggttg cgtaaaaaac ggctgaccgt cattttggtc
    23701 acgcctacac ggtcagccac atcctgaagt acgggtcttt tctttttcat cgtcctgaag
    23761 gtacaaaaga gatagattct cttagtttaa cacggacgca tcagaacctt cccggggaaa
    23821 acagtgcttg tttacaatta ttagatatac actaaatcaa ttgcatcgca ttgtgctaac
    23881 gacgcggcaa aaccccggaa gcgtgcatca gcacgcgacc ggagcgagcg aggaagcctg
    23941 caaaaaggca ggcttcaaaa atgccgtgga gctttaaacc ggcggcagat cgaacagtaa
    24001 cacttcgctg tcgctatccg catggatgga gattgcctgc tcatcccaga ttgccagacc
    24061 atcgctggtc gaggctttca cgccgttaat ggtgacattg cctttcacca cctggatcca
    24121 gacgcggcgt tcagcggcaa tctgatgcac cgactgctca tctttcagca acgcccagcg
    24181 gtacagttcc atatcctgat gcactttcaa tgaaccatct cgcgcatccg gcgagagcac
    24241 cagctgtttg ccctgtacgg catcgaagcg acgctgttca taacgcggcg taataccgtt
    24301 ttcttcaggc atgatccaga tctgatacag atgcagacgc tcggtgctgc ttgggttgta
    24361 ctctgagtga cgaatacccg tacccgcact cataatctgg aactcacccg ccggaacctg
    24421 ctctttattg cccatgctgt cctgatgctc aacagtacct tccagcacgt aggtcaaaat
    24481 ttccatatct ttatgcgggt gagtgccgaa gccctgccct gcttcaatca cgtcgtcgtt
    24541 aatcacgcgc agcgcggaga agcccataaa gttcggatcg taatagttgg caaaagagaa
    24601 agtatgccag gagtccagcc agccatgatt tgcatgacca cgttcatttg ctttgcgtaa
    24661 gtagatcatt tgtattcgcc ccctgaatga tttcgatggg cttagtgtgg acccgatctg
    24721 cctgggatga tagagggtga aaattgaccc ctctgttcaa aaaaattgaa ctattcgagg
    24781 ggccatcaaa cttaattcgc gagagtcact gtggagggag aggcttgctc aaatccgcgt
    24841 tcaaggattt ccagattggt aagaacttca gattccttga cgtaatttgg cgcaccgtgg
    24901 gtgatggttt gatacaacgc atcataaacg cgcccgtaat cgcccatctc cggcttcatc
    24961 tcttctctga ccgtcacgcc ctcgtcattg acatactcca gcacaccgac cgaattatcc
    25021 gctgcgaatc ccggttcgcc cggcataata ttagccttca ggctggtttc ctgctggtcg
    25081 ataccgtatt taataaacga acctttctta ccgtgaacga taaatttcgg ataatcgatt
    25141 ttcaccagat ggctggtttt gacgatggct ttcaggtcgc cataaaacag ttgcgcttca
    25201 aaggtgtcgt caggattggc tttattacgc aggctgcgga tgtcataagc gacgtgatcc
    25261 gggcgaccga acagagaaat aatctggtcc atcgtatgca caccaaggcc ataaaacgcg
    25321 ccatcctgcg gcagcccagg tttggtttct gccaccgggc ggtaatagtc aaaatggctt
    25381 tccacttcaa caatctctcc caacttgcca ctttcaatcg cttttttcgc tgtcaggaag
    25441 caggagtcaa agcgacgatt ctgatacggc gtgacggtca gccctttgct tttcgccaac
    25501 gcaaacagct ctttcgcctg cgcaagtgtc ggagtgaacg gtttttcgac cagcacattt
    25561 ttcccggctt ccagcgcgcg cttcgcgtac tcgaagtggc tgtccgcgtg ggtgcagaca
    25621 acaaccagct taacatcggg atcgtttagt acttcgtcga gatcgctggt gaaatggata
    25681 tgggaataaa tgggagcctg ttcttccggc ttcgcatggc gacgaaaaat atgcgcgaca
    25741 tgccagctat ccttgcggtt aagtacatac ggcagatggt aacgggtggt gcttttgccg
    25801 aagccaataa aggcgcagtt gatgaccatg atttcgtcct ttttaaggtg gttatttaca
    25861 ccttagcgca aagcggacgt ggttcctact ggagtgcgca taacgaacac aagcactccc
    25921 gtggataaat tgagaacgaa agatcaaaaa aaaagccagc acccggctgg ctaagtaata
    25981 ctggaagcaa tgtgagcaat gtcgtgcttt caggttctcc gcgagggtct tcctgatcgc
    26041 gagacaataa taatcattct cattcgcact tgtccaacac tttttgcaaa aaaatgcatt
    26101 tgactcgcat ttgaaagtca atgatgttga aagggacatt taccccaaag aggacaaagg
    26161 aatgagtgag atagtaatac gccacgcaga aacacgggat tacgaggcca tcaggcagat
    26221 tcacgcccag ccggaggtgt attgcaacac actacaggtg cctcatcctt ccgatcatat
    26281 gtggcaggag cgactcgccg atcgtcccgg catcaagcaa ctcgtcgcct gtattgatgg
    26341 agacgtcgtg ggccatctca ccattgacgt gcaacagcgc ccacgccgca gtcatgttgc
    26401 cgattttggt atctgtgtcg actctcgttg gaagaaccgc ggcgtcgcca gcgccctgat
    26461 gcgagagatg attgaaatgt gcgacaactg gttgcgggta gatcgcattg aactaaccgt
    26521 gtttgtcgat aacgccccgg caattaaggt ctataaaaaa tacggctttg aaattgaagg
    26581 gactggtaag aagtacgcat tgcgtaatgg tgaatatgtc gatgcatatt atatggcgcg
    26641 ggtgaagtaa gatagtgccc tttttctgag atggaaaaag ggtgtcattc aaaatcgaca
    26701 taccttcctt taaggtattt atttgccca
//
LOCUS       DAZGJW010000061        26261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZGJW010000061 DAZGJW010000000
VERSION     DAZGJW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.061"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            64..825
                     /gene="adiY"
                     /locus_tag="ACHKIK_002508"
     CDS             64..825
                     /gene="adiY"
                     /locus_tag="ACHKIK_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO9083664.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            962..2299
                     /gene="adiC"
                     /locus_tag="ACHKIK_002509"
     CDS             962..2299
                     /gene="adiC"
                     /locus_tag="ACHKIK_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO9083665.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDVPISKD"
     gene            2403..4046
                     /gene="eptA"
                     /locus_tag="ACHKIK_002510"
     CDS             2403..4046
                     /gene="eptA"
                     /locus_tag="ACHKIK_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO9083666.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4043..4711
                     /gene="basR"
                     /locus_tag="ACHKIK_002511"
     CDS             4043..4711
                     /gene="basR"
                     /locus_tag="ACHKIK_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO9083667.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4712..5812
                     /gene="pmrB"
                     /locus_tag="ACHKIK_002512"
     CDS             4712..5812
                     /gene="pmrB"
                     /locus_tag="ACHKIK_002512"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO9083668.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5788..5877)
                     /gene="pmrR"
                     /locus_tag="ACHKIK_002513"
     CDS             complement(5788..5877)
                     /gene="pmrR"
                     /locus_tag="ACHKIK_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO9083669.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5989..7491)
                     /gene="proP"
                     /locus_tag="ACHKIK_002514"
     CDS             complement(5989..7491)
                     /gene="proP"
                     /locus_tag="ACHKIK_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO9083670.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7755..8633)
                     /gene="rdcB"
                     /locus_tag="ACHKIK_002515"
     CDS             complement(7755..8633)
                     /gene="rdcB"
                     /locus_tag="ACHKIK_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO9083671.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLA"
     gene            complement(8630..10858)
                     /gene="crfC"
                     /locus_tag="ACHKIK_002516"
     CDS             complement(8630..10858)
                     /gene="crfC"
                     /locus_tag="ACHKIK_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO9083672.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDRLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALASANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGAVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQRTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            11261..11596
                     /gene="yjdM"
                     /locus_tag="ACHKIK_002517"
     CDS             11261..11596
                     /gene="yjdM"
                     /locus_tag="ACHKIK_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO9083673.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            11756..12199
                     /gene="yjdN"
                     /locus_tag="ACHKIK_002518"
     CDS             11756..12199
                     /gene="yjdN"
                     /locus_tag="ACHKIK_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO9083674.1"
                     /translation="MSLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            12332..13120
                     /gene="phnC"
                     /locus_tag="ACHKIK_002519"
     CDS             12332..13120
                     /gene="phnC"
                     /locus_tag="ACHKIK_002519"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO9083675.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            13145..14161
                     /gene="phnD"
                     /locus_tag="ACHKIK_002520"
     CDS             13145..14161
                     /gene="phnD"
                     /locus_tag="ACHKIK_002520"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9083676.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            14267..15046
                     /gene="phnE"
                     /locus_tag="ACHKIK_002521"
     CDS             14267..15046
                     /gene="phnE"
                     /locus_tag="ACHKIK_002521"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO9083677.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRVINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            15067..15792
                     /gene="phnF"
                     /locus_tag="ACHKIK_002522"
     CDS             15067..15792
                     /gene="phnF"
                     /locus_tag="ACHKIK_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO9083678.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            15793..16245
                     /gene="phnG"
                     /locus_tag="ACHKIK_002523"
     CDS             15793..16245
                     /gene="phnG"
                     /locus_tag="ACHKIK_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO9083679.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            16242..16826
                     /gene="phnH"
                     /locus_tag="ACHKIK_002524"
     CDS             16242..16826
                     /gene="phnH"
                     /locus_tag="ACHKIK_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO9083680.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            16826..17890
                     /gene="phnI"
                     /locus_tag="ACHKIK_002525"
     CDS             16826..17890
                     /gene="phnI"
                     /locus_tag="ACHKIK_002525"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO9083681.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLVKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            17883..18728
                     /gene="phnJ"
                     /locus_tag="ACHKIK_002526"
     CDS             17883..18728
                     /gene="phnJ"
                     /locus_tag="ACHKIK_002526"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO9083682.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            18725..19483
                     /gene="phnK"
                     /locus_tag="ACHKIK_002527"
     CDS             18725..19483
                     /gene="phnK"
                     /locus_tag="ACHKIK_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO9083683.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMGEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            19594..20274
                     /gene="phnL"
                     /locus_tag="ACHKIK_002528"
     CDS             19594..20274
                     /gene="phnL"
                     /locus_tag="ACHKIK_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO9083684.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            20271..21407
                     /gene="phnM"
                     /locus_tag="ACHKIK_002529"
     CDS             20271..21407
                     /gene="phnM"
                     /locus_tag="ACHKIK_002529"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO9083685.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CHARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            21407..21964
                     /gene="phnN"
                     /locus_tag="ACHKIK_002530"
     CDS             21407..21964
                     /gene="phnN"
                     /locus_tag="ACHKIK_002530"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO9083686.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            21951..22385
                     /gene="phnO"
                     /locus_tag="ACHKIK_002531"
     CDS             21951..22385
                     /gene="phnO"
                     /locus_tag="ACHKIK_002531"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO9083687.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            22387..23145
                     /gene="phnP"
                     /locus_tag="ACHKIK_002532"
     CDS             22387..23145
                     /gene="phnP"
                     /locus_tag="ACHKIK_002532"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO9083688.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(23292..23621)
                     /gene="yjdP"
                     /locus_tag="ACHKIK_002533"
     CDS             complement(23292..23621)
                     /gene="yjdP"
                     /locus_tag="ACHKIK_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO9083689.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(23690..24007)
                     /locus_tag="ACHKIK_002534"
     CDS             complement(23690..24007)
                     /locus_tag="ACHKIK_002534"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFO9083690.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            24260..26245
                     /gene="yjcS"
                     /locus_tag="ACHKIK_002535"
     CDS             24260..26245
                     /gene="yjcS"
                     /locus_tag="ACHKIK_002535"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO9083691.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         6220 a         7361 c         6903 g         5777 t
ORIGIN      
        1 atttcccggt atcgctttgc tttagcgaga actggtcttt tatttgcact caggagtaca
       61 tgtatgagga tttgcagcga ccaaccttgt attgttttat tgactgaaaa agatgtctgg
      121 ataagggtga atgggaaaga acctattagc cttaaagcta accatatggc gttattaaat
      181 tgtgaaaata atattatcga cgtctcctct cttaacaaca ctttggttgc tcatattagt
      241 cacgacatca tcaaagatta cctccggttt ctgaataaag atctctcgca aataccagta
      301 tggcaacgta gcgctacgcc catactcacc ctgccatgcc tgacgccaga cgtctttcgc
      361 gttgccgcgc aacacagcat gatgcccgca gaaactgagt cagaaaagga acgaacacgc
      421 gcattattat tcacggtgct atcccgtttt ctcgacagta aaaaattcct ttcattaatg
      481 atgtatatgt tacgtaattg tgtaagtgac agcgtttatc aaattattga aagcgatatt
      541 cacaaagact ggaatcttag tatggtagcc agttgtttat gtcttagccc aagtctgtta
      601 aagaaaaagt tgaaaagcga aaacaccagt tatagccaaa taatcaccac ctgccgcatg
      661 cgttatgccg taaatgaatt aatgatggac ggtaaaaata tctctcaggt atcacagtcc
      721 tgcggctaca acagtacgtc gtactttatt tctgtcttta aagacttcta cggtatgacg
      781 ccgctgcatt atgttagtca gcacagagaa cgcactgtcg cctgattttt aaccttaacg
      841 aagagctata ttaataacgg catcagcgat aacccggtcg ataataattc aactatcgaa
      901 tgcaggcgta tgatatgacg taatttattg tcacgaagct cgccttcgca ggagtttaat
      961 tatgtcttcg gatgctgatg ctcacaaagt gggcttaatc cccgtcaccc tgatggtgtc
     1021 ggggaatatt atggggtcag gtgtttttct gttacctgca aacctggcct ctactggcgg
     1081 gattgccatt tatggatggt tggtgacgat tatcggtgcg ctggggctct cgatggtata
     1141 cgccaaaatg tcgttcctcg atccaagtcc tggtggttct tacgcgtacg cccgccgctg
     1201 ctttggcccg tttctcggtt atcaaaccaa cgtcctctac tggctggcct gctggatcgg
     1261 caatatcgcc atggtggtca ttggcgtagg atatttaagt tacttcttcc cgattctgaa
     1321 agatccattg gtattaacca tcacctgcgt cgtggtgctg tggatcttcg tcctgctgaa
     1381 cattgtcggt ccgaaaatga tcacccgtgt gcaggccgtt gccaccgtgc tggcgctgat
     1441 ccccatcgtc gggattgccg tatttggctg gttctggttc cgtggtgaaa cctatatggc
     1501 ggcatggaac gtcagcggcc tgggcacctt cggtgcaatt caaagtaccc ttaacgttac
     1561 gctgtggtcg ttcatcggtg tggaaagtgc ctccgttgcc gcaggtgtgg tgaaaaaccc
     1621 gaaacgcaat gtccctatcg ccaccattgg tggggtattg attgccgccg tttgctatgt
     1681 actttctacc accgcgatta tggggatgat ccctaatgcc gcactgcgcg tttctgcttc
     1741 gccattcggt gatgccgcac ggatggcgtt gggtgacacc gccggggcca ttgtttcctt
     1801 ctgcgcagct gcgggttgct taggttcact gggcggctgg acgttgctgg cgggtcaaac
     1861 ggcgaaagcc gctgccgatg acggactgtt cccaccgatt tttgcccgtg taaataaagc
     1921 gggcacgcca gtggcggggc tgattatcgt cggtattttg atgaccatct tccagctcag
     1981 cagcatttca ccaaacgcga ccaaagagtt cggtctggtt tcttccgtct cggtcatctt
     2041 tacactggtg ccatatcttt acacctgtgc ggcgttactg ctgctcggtc acggtcactt
     2101 tggtaaagca cgcccggcat atctggctgt tactaccatt gccttcctct actgcatttg
     2161 ggccgtggtg gggtccgggg cgaaagaggt tatgtggtca tttgtcaccc tgatggtaat
     2221 caccgccatg tatgcgctga attacaaccg gctgcataaa aacccgtatc ccttagatgt
     2281 accaataagc aaagattaat tccccttaat ccagcaaaca taaaagccaa ccttaagaac
     2341 ttaaggttgg cttaattttg ctttgcgagc atatgcgcac tttgttcgat ggaaacaccg
     2401 tgatgttgaa gcgcctacta aaaagaccct ctttgaattt actcgcctgg ctattgttgg
     2461 ccgcttttta tatctctatc tgcctgaata ttgccttttt taaacaggtg ttgcaggcgc
     2521 tgccgctgga ttcgctgcat aacgtactgg ttttcttgtc gatgccggtc gtcgctttca
     2581 gcgtgattaa tattgtcctg acactaagct ctttcttatg gcttaatcga ccactggcct
     2641 gcctgtttat tctggttggc gcggctgcac aatatttcat aatgacttac ggcatcgtca
     2701 tcgaccgctc gatgattgcc aatattattg ataccactcc ggcagaaagt tatgcgctga
     2761 tgacaccgca aatgttatta acgctgggat tcagcggcgt gcttgctgcg ctgattgcct
     2821 gctggataaa aatcaaacct gccacctcgc gtctgcgcag tgttcttttc cgtggagcca
     2881 atattctggt ttctgtacta ctgattttgc tggtcgccgc actgttttat aaagactacg
     2941 cctcgttgtt ccgcaataac aaagagctgg tgaaatcctt aagcccctct aacagcattg
     3001 ttgccagctg gtcatggtac tcccatcagc gactggcaaa tctaccgctg gtgcgaattg
     3061 gtgaagacgc ccaccgcaac ccgttaatgc agaacgaaaa acgtaaaaat ttgaccatcc
     3121 tgattgtcgg cgaaacctcg cgggcggaga acttctccct caacggctac ccgcgtgaaa
     3181 ctaacccgcg gctggcgaaa gataacgtgg tctatttccc taataccgca tcttgcggca
     3241 cggcaacggc agtttcagta ccgtgcatgt tctcggatat gccgcgtgag cattacaaag
     3301 aagagctggc acagcaccag gaaggcgtgc tggatatcat tcagcgagcg ggcatcaacg
     3361 tgctgtggaa tgacaacgat ggcggctgta aaggtgcctg cgaccgcgtg cctcaccaga
     3421 acgtcaccgc gctgaatctg cctggtcagt gcatcaacgg cgaatgctat gacgaagtgc
     3481 tgttccacgg gctggaagag tacatcaata acctgcaggg tgatggcgtg attgtcttac
     3541 acaccatcgg cagccacggt ccgacctatt acaaccgcta tccgccgcag ttcaggaaat
     3601 ttaccccaac ctgcgacacc aatgagatcc agacctgtag caaagagcaa ctggtgaaca
     3661 cttacgacaa cacgctggtt tacgtcgact atattgttga taaagcgatt aatctgctga
     3721 aagaacatca ggataaattt accaccagcc tggtttatct ttctgaccac ggtgaatcgt
     3781 taggtgaaaa tggcatctat ctgcacggtc tgccttatgc catcgccccg gatagccaaa
     3841 aacaggtgcc gatgctgctg tggctgtcgg aggattatca aaaacggtat caggttgacc
     3901 agaactgcct gcaaaaacag gcgcaaacgc aacactattc acaagacaat ttattctcca
     3961 cgctattggg attaactggc gttgagacga agtattacca ggctgcggat gatattctgc
     4021 aaacttgcag gagagtgagt gaatgaaaat tctgattgtt gaagacgata cgctgttatt
     4081 gcagggattg attctggcgg cgcaaactga aggctacgcg tgcgatggcg tgacaaccgc
     4141 gcggatggcg gaacaaagcc ttgaggccgg tcattacagc ctggtggtac tggatttagg
     4201 gttacccgac gaagatggac tgcattttct cgcccgtatc cggcagaaaa aatacaccct
     4261 gccagtactg atcctcaccg ctcgcgatac gctgaccgac aaaatcgctg ggctggatgt
     4321 cggtgccgat gactatctgg tgaagccttt tgcgctggaa gagttacacg cccgtatccg
     4381 cgccctgcta cgacgccata ataatcaggg cgaaagtgag ctgattgttg gcaatctgac
     4441 gctgaatatg ggtcgccgtc aggtatggat gggcggtgaa gagttgattc tgacgcccaa
     4501 agaatatgct ctgctgtcac ggttaatgct caaagcaggc agtccggtgc atcgggaaat
     4561 tctctacaac gacatctata actgggacaa tgaaccctcg accaacaccc tggaagtgca
     4621 tatccacaat ctgcgcgaca aagtgggcaa agcccgtatc cgcaccgtgc gcggctttgg
     4681 ctatatgctg gtcgcgaatg aggaaaacta attgaatctg atgcattttc tgcgccgacc
     4741 aatatcgctg cgccaacggc tgatattgac catcggggcc attttgttgg tgtttgagct
     4801 gatcagcgtc ttctggctat ggcatgaaag taccgagcag attcagctgt ttgagcaggc
     4861 gctacgcgac aatcgcaaca acgatcgcca catcatgcgt gagatccgcg aggcggtcgc
     4921 cagcctgatt gtccccggcg tctttatggt cagcctgacg ctatttatct gctatcaggc
     4981 ggtgcgccgc atcacccgcc cgctggcgga gctgcaaaaa gagctggaag cgcgcactgc
     5041 cgacaactta acgcccattg ccattcacag cgccaccctc gaaatcgaag cggtggtttc
     5101 ggcgttaaac gatctggtca gtcgcctgac cagcacgctg gataacgaaa ggttgtttac
     5161 cgctgacgtc gcgcacgaac tgcgaacgcc actggcgggg gtgcgtttgc atctggaact
     5221 gctggcgaaa acgcatcaca ttgatgtagc accgttagtg gcacgacttg atcagatgat
     5281 ggagagcgtc tcccagctgc tgcaactggc gcgtgccgga cagtcatttt cttccggtaa
     5341 ttatcaacat gtaaaactgc tggaagatgt cattctcccc tcgtatgacg aactcagtac
     5401 catgctcgac cagcgacagc aaaccctgct attgccagag agcgccgccg acatcaccgt
     5461 tcagggtgat gcaaccctgc tgcggatgtt attgcgaaac ctggtagaaa acgcccatcg
     5521 ttacagcccg caaggcagca acattatgat taagctgcaa gaagatggcg gagcggtcat
     5581 ggcagttgaa gatgaaggac caggtattga tgagagtaaa tgcggggagt tgagtaaagc
     5641 gtttgtacgt atggacagcc gttatggcgg gattggtctg gggttaagta ttgtcagccg
     5701 cattacacag ttgcatcacg ggcagttttt cctgcaaaac cggcaagaga cttccggcac
     5761 gcgggcctgg gtacggctga agaaagatca gaacgtggca aaccagatat aaagaaagct
     5821 gctgaccacc agcacgctga acacggtagt taaactttca taaacacggt ttttcattgc
     5881 actctcctcc gttaacctgg aggagagtat gcgcgtcaga gattaagtga accttaagag
     5941 ttcaattggc ctgataagac agcgtcacat caggccatcc gtttcagctt attcatcaat
     6001 tcgcggatgt tgctgcacca ggcgggtacg tttcgcctgc aaatcggcaa tctcgtggtc
     6061 aatattatcg attttctgct cgatattatc gtaatgctcg acgagaattt ccttcgcttc
     6121 ctgtatatct gacgccgccg gtgtcgcacc tttcaacgga cgatttgccg tctctttcat
     6181 ggttacgccg gtgattaaac caatcaccgc cactaccatc aggtaatagg caggcatcat
     6241 cagattctgc gagctttcga ccagccaggc cgccagtgtt ggcgtcagac cggcaaccag
     6301 caccgaaata ttaaatgccg ccgccagcgc gctgtaacgg atatgcgtcg ggaacatcgc
     6361 tggcaaggta gaagccataa cgcccgtaaa gcagttaagg atcaccgcca gcatcagtaa
     6421 cccggcaaaa atcaggccga tgacgttact gttaatcaga ataaacgccg ggatcgccaa
     6481 cacaaacagg gcaacactac caagtagcac aaacggacga cggccaaaac ggtcactcag
     6541 caagcccatc accggctgga caaacagcat accgatcata atggcgataa taatcagcac
     6601 cccgtggtct tcggagtaat gcaggttatg cgacaaataa ctcggcatat aggtcagcaa
     6661 catgtagtaa gtcacgttgg tggcaattac cagaccaata catgtcaaca ggctgcgcca
     6721 gtgtttggtg gcaatctctt taaacgagac tttcgggcca tcctgcaaac cttcgcggtc
     6781 gccctgttcc agtttatcga catgctgctg gaacgccgga gtttcttcca gcgcatggcg
     6841 caggtaaagc ccgataatcc ctaacggcag agcaataaag aacggaatac gccagcccca
     6901 gtcgaggaag ttcgcttcgc cgacaatggt cgaaattaac accactacgc cagcacccag
     6961 cacaaacccg gcaatagaac cgaagtccag ccagctgccc ataaagccac gtttacggtc
     7021 aggtgagtat tccgcaacaa atatcgacgc cccggtatat tcaccgccga ccgagaaacc
     7081 ttgtgccatc ttacagatca acagcagaat cggtgcccaa ataccaatcg tgtcgtagga
     7141 cggtattaag ccaatacaga acgtactgat cgacataatc acaatagtga tagcgaggat
     7201 cttctggcga ccatatttat cgcccaacat accaaagaag agcccgccaa gcggtcgaat
     7261 cagaaaggga acggagaaag tggcaagtgc agcaaccatc tgcacgctgg ggtcagcccc
     7321 cgggaaaaaa actttaccta atgcgtaagc aacaaaacca taaacaccaa aatcaaacca
     7381 ttccattgca ttacccagtg atgctgcggt aatggcttta cgcagtttac cgtcatcaat
     7441 aatggtgaca tcacgaaggg taattggttt tacttttttc cttttcagca tagctttcct
     7501 cgcagagttg gcccatttcc gcaaacgcca gaaaaatcag cgcgacggcc gcaaaagggg
     7561 agcgggaatt gcaggatgca atctctgtaa caagcgtagc gggtttgctt acaccctccg
     7621 gttaaggaat gtacaatcaa accagttgac gcctgaattg ggcagttaat gaccttttta
     7681 ccctaccagt gtttaaattt gtgatcaact tcacacatgg atttcgttta ggactcattg
     7741 atgtaactga ttatttacgc caaatttaca gagaagattt cctcgagcat tgcccccgcc
     7801 agacgctgcg ctgaacagag tcgtggcatc cccagcgcga cagtttgcca gcgttgggtc
     7861 actgaccagc aaacgggttg tttatctgcc gtggtccact ttcccagcca acttaaaaaa
     7921 tcagcatgat ccccgcccgt cggaacggtc gaagaggcaa accactgatg gtaatagtga
     7981 cgcgtggtcg gggccgcatt gatactctct gccagatgtt gtgtcgcttt cacgcgcaaa
     8041 tcacccagca acaacgccac cgcttgcggg cttcccagac gacaggcatc gccaaatgcc
     8101 ccccaacgca actcttcgag aacgataaaa cagcgcccag gcaacgacca ggcgttatac
     8161 gccccagcac gccagcgggt aaaaatttgc tcgctctgat cacctgcctg caccagtagg
     8221 cctcgttgcg ttaacgcctt ttccttccag cggcaacggg ctgaaaacca ctcatacaag
     8281 gcggcaattt gctgggggag ctccgggaaa gcagagtgct ggcggtgatc cattttttgc
     8341 agcagcgcca gatgctgcgc gatctgcgtc agtgccagat gaccgggatc cagcatcgac
     8401 gccagttttt ccaccaatgt cagacgcagg ctaagcggcg catttaacat gccgccgttc
     8461 gtccatgtct gaagctgcac gcgcgcaaga agttcctgca tcagtcgttc acgaaactgc
     8521 tgctgatggg cttgtggaag gcgggcatca tcgccctgcg ccagatccac taaaaagcgg
     8581 ggataaaccg actccagcac gcgaccgggg ccgtcaagta acgtcttggt caatatcgtt
     8641 ctgccgtgaa aagtgtttga atatcatcgc gtaacagctg ggcgtcggtg taaatccagc
     8701 cgtgagtcat cacagtccgc tgcaattgct gctgcatcag cctgaccacc gattcatttt
     8761 gttgacgcag agccagactt tcgcgtaaac gcgtctgtaa ttccgtcaaa catgaagcga
     8821 actcagcgaa aaaagtattc atgcctgccg taacagattc atcgacctgc tctgccagaa
     8881 ctttagccat ttgttggcaa taaagatcga cttctgcgct taaagctcgt tgcaacacac
     8941 tgtaatcaac cgtctctgtc ggggatttct catttccccg tccccagtcg ggctgattca
     9001 accagcgcga aaaagtctca cgcacaacgc ctaaacgcgt gctctgctcg tccgttgctt
     9061 cctggcgcga aatgacttca ctgaacagct ggcgagtgtt gaagtgggga actgcgccgt
     9121 gaaaaacagg aaaatgaaac ccaggacgaa accctgactc gctcaattcc attttgactt
     9181 gttgctcaat ggggcgaata acatcggtta acacccggca aagggtggat tccagctcgg
     9241 caaaacgcag cgtaaagtcg cgactgatgg tgttctgcgc cgtctgtaac agtgtctcac
     9301 agcgggtacg aatctcgctt aacggctccg aatcatcctg aaacaacgcg gctaactgtg
     9361 cattcaccgc atcttgctgt tgacgcagaa agtggttggc ggaggccaag gccagctcga
     9421 tttcatgttt aatctcgccg ctcacctgcg cctgattgag ttgcaatagc tgcaaacttt
     9481 cttcgacctg atggatattt tgccgcaatt gttcacaagc gacgtttaac ccgtgcgcac
     9541 gaaaatccag gtattcccgc gcctgctgcg cgtaattcaa cagtttatgc gcggcagatc
     9601 gcagagcata caacgaggcg ttagcgtaag cggcatgaag caacgcctga attggctggg
     9661 cgaacagcga atcttcccac aactgatcgg cagcatgacg aatatgttcg aggtccgcca
     9721 gatcggcatg acgccagcgc ctgccgagcg cggcatgggc aaaatcttcc acccagcgtt
     9781 gttgctctgg cggtggtaac ttaccgttgt tggctaactc atggcgcgcc cgattcgcca
     9841 ggtagcccca catcgacgac accggaaata tctgctgtgg cgtaatacag cctttcatca
     9901 gcgtcccgga aatcagtgcc cgcacctggt cggcgtcgtc actgttacga tcctgttgat
     9961 cgaacttatt gaccagcaca tacagcggca ccgattgccc caccgccaaa atcgcctcac
    10021 ggacctcttc atcggagatc gatttcagtt gcgtataatc cagcaccgcc agtaccgccg
    10081 aggcgcgtgc cagctgctgg ttaagcattt tttgcagatg cggttgcccg gcttcatttg
    10141 gcccgggggt atccagtaac gtcaactgac cgggataact ttccagcccc gccagatgga
    10201 caaactccac ttcaatcacg ggaatatgct caatggcggc gtaagcagaa aaaggaaaat
    10261 cgacgtccag cgccttcgcc agtcgcacta aatcattcaa acttttcaga caatgaaaaa
    10321 taggctgggc acccagataa tatttttcga aagcgacgcc attttcgatc cgctgcataa
    10381 gcgcacgcat atctttatct atttccagca catcggtcag atgcttaata tcgcaatcac
    10441 gcaggcgctg ttgtaatttt tgaattaaac ggtcgattgg cgcgacatgt gaaaaatgca
    10501 gtaccggttc tttttgcccg ggcgtatggc gaataagcgt cggcagcgca gtcattgggc
    10561 gattacgatt aggcaaaacc tccgtaccaa caatggcatt aatggtggtt gatttccctg
    10621 ctttcatggt accgacaatc gccagcacca tttccagtcg ggaaatttta cgcaactcat
    10681 tattcagcat cgcgtgacgt tcggcgatat taggctgact ccagggtaaa gccagttgtg
    10741 gcgcatcgtc tccgggtaca gagagaggca ttttttccag taactgcaac tgttggcgag
    10801 aaagctgtaa caggcgttca gcctcctgac ttaactcata cagggtctgt gtgtacatag
    10861 aaaattcttc cttaaagcaa attttgttac ttttatttag ccagattgtt tttgagttct
    10921 gttttcggct tttataatta ctgcaagaaa taattttata tttagcgtgt tgttttttat
    10981 tagaataaat aacgtcttcc gatacgttta aaacatcaga aacataaaaa tatcatgtga
    11041 attaaaaaaa agaacaagta gagcattaac attatcttaa ataataaatg gaggcaaaaa
    11101 gattattttc tgtttgcgtt tcctttcaaa tgaaaacgat cgtcgtctaa aatcagcagt
    11161 acccccgaca aactcaggga ttttgtgtat aattgcggcc tttttcggca atctgccgtt
    11221 ttttggcgct tttgccctgc tgacttttga ggaaatccac atgtcattac cacactgccc
    11281 aaaatgcaac tccgaataca cttacgaaga taacggcatg tacatctgcc cggaatgtgc
    11341 ctacgaatgg aacgacgcag aacctgcaca ggaaagcgac gagctgatcg ttaaagatgc
    11401 taacggcaat ctgctggctg acggcgacag cgttaccatc attaaagatc tgaaggtgaa
    11461 aggtagctct tcgatgctga aaattggcac caaagtgaaa aacatccgcc tggttgaagg
    11521 cgaccataac atcgattgca aaatcgacgg ttttggtccg atgaaactga aatctgagtt
    11581 tgtgaaaaag aactgattgt attgtgatcg gtaagccgga taaggcgctc gcgccgcatc
    11641 cggcaacgat gccgactgcc tgatgcgacg ctgaccgcgt cttatcaggc ttccctctca
    11701 tctgtataaa tttcgaacta cacttaactg gcttctctta actgaggtca ccatcatgtc
    11761 gttaagtccc tacctctctt ttgccggtaa ctgtgccgac gcaattgctt attatcaacg
    11821 tacgttgggc gctgaactgc tctataaaat cagcttcggc gaaatgccaa aatcagcgca
    11881 ggacagcgcc gagaactgcc cttccggaat gcaatttccc gataccgcca tcgcccacgc
    11941 caacgtgcgc attgccggaa gcgacatcat gatgagcgat gctataccgt caggaaaagc
    12001 cagctactcc ggctttacgc tggtgctcga ttcgcaacag gtcgaagaag gaaaacgctg
    12061 gtttgacaat cttgccgcta acggaaaaat cgaaatggcc tggcaggaaa ctttctgggc
    12121 gcatggcttt ggcaaagtca ccgataaatt tggcgtaccg tggatgatta atgtcgtcaa
    12181 acaacaacca acgcaataac ccgccgggag gcccgccctc ccgcactgtc atcgaattcc
    12241 cgttaactct tcatctgtta gtcactttta attaaccaaa tcgtcacaat aatccgccac
    12301 gatggagcca cttttttagg gaggctgcat catgcaaacg attatccgtg tcgagaagct
    12361 cgccaaaacc ttcaatcagc atcaggcgct gcatgcggtt gatctgaaca ttcatcacgg
    12421 tgaaatggtg gctctgcttg ggccgtcggg ttccggcaaa tccacccttt tacgtcactt
    12481 aagcggtttg attaccggcg ataaatccgc cggcagccat atcgagctgc tgggccgcac
    12541 agtccagcgc gaaggccgtc tggcgcgcga tatccgcaaa agccgcgcca acaccggcta
    12601 catcttccaa caattcaacc tggtgaaccg cctgagcgta ctggagaacg tgctgattgg
    12661 cgcgctcggc agcacgccgt tctggcgcac ctgttttagc tggttcaccg gcgagcagaa
    12721 acagcgcgcg ttacaggcgc tgacccgcgt tggcatggtg cattttgccc atcagcgcgt
    12781 ttccaccctc tccggcggac agcagcagcg tgtggcgatt gcccgcgcgc tgatgcagca
    12841 ggcgaaggtg attctggccg atgaacccat cgcctcgctg gacccggaat ccgcccgcat
    12901 cgtgatggac accctgcgcg acatcaatca gaacgacggc atcaccgtgg tcgtcacgct
    12961 gcatcaggtg gattacgccc tgcgctactg cgaacgcatc gtcgccctgc gccaggggca
    13021 cgttttctac gacggcagca gccaacagtt tgataacgaa cgttttgacc atctctaccg
    13081 cagcattaat cgcatcgaag agaacgcgaa agctgcctga catccccatc attgaggaaa
    13141 acgaatgaac gctaagataa ttgcctcgct ggccttcacc agcatgttca gcctcagcac
    13201 cctgttaagc ccggcacacg ccgaagagca ggaaaaggcg ctgaatttcg gcattatttc
    13261 aacggaatca cagcaaaacc tgaaaccgca atggacgcca ttcttacagg atatggagaa
    13321 gaagctgggc gtgaaggtga acgccttctt tgccccagac tacgcaggca ttatccaggg
    13381 aatgcgcttc aataaagtgg atatcgcctg gtacggcaac ctgtcggcaa tggaagcggt
    13441 ggatcgcgcc aacggccagg tcttcgccca gacggtcgcg gcggatggat cgccaggtta
    13501 ctggagcgtg ttgatcgtca acaaagatag tccgatcaac aacctgaacg atctgctggc
    13561 gaagcggaaa gatctcacct tcggcaatgg cgatcctaac tccacctctg gcttcctcgt
    13621 ccccggttac tacgtctttg ccaaaaacaa tatctccgcc agcgacttta agcgcaccgt
    13681 caacgctggg catgaaacca acgcgctggc cgtcgccaac aagcaggtgg atgttgccac
    13741 caacaacacc gaaaacctcg acaagctgaa aacctccgcg ccagagaagc tgaaagaact
    13801 gaaggtgatc tggaagtcgc cgctgatccc aggcgatccg atcgtctggc gtaaaaacct
    13861 ttccgaaacc accaaagaca agatctacga cttctttatg aactacggca aaacgccgga
    13921 agaaaaagcg gtgctggaac gcctgggctg ggcgccgttc cgcgcttcca gcgacctgca
    13981 actggtgccg attcgccagc tcgcgctgtt taaagagatg cagggcgtga aaagcaataa
    14041 aggactgaat gagcaggaca agctggcaaa aaccaccgag attcaggcgc agctggatga
    14101 cctggaccgc ctgaacaacg cgttaagcgc gatgagttcg gtgagtaaag cggtgcagta
    14161 aatcgtaggt cggataagac gccccggcgt cgcatccgac aatgtgcagg cgttgatgcc
    14221 ggatgcggtg caagcacctt atccggccta cagaccggag ccaaacatgc aaaccatcac
    14281 catcgcccca cccaagcgca gctggttctc gcttctgagc tgggccgttg ttctcgccgt
    14341 gttggtcgtc tcgtggcagg gcgcggaaat ggccccgctc acgctgatta aagacggcgg
    14401 caacatggcg acgttcgccg ccgacttctt cccgcccgat ttcagccagt ggcaggatta
    14461 cctcaccgaa atggccgtca cgctgcaaat cgccgtctgg ggcacggcgc tggcggtggt
    14521 tctctccatc cccttcggcc tgatgagcgc cgaaaacctg gtgccgtggt gggtttacca
    14581 gcccgttcgc cgcctgatgg acgcctgccg cgtcattaac gaaatggtct tcgccatgct
    14641 gttcgtggtc gccgtcggcc tcggcccgtt cgctggcgtg ctggcgctgt ttatccacac
    14701 caccggcgtg ctctccaaac tgctttccga agcggtggaa gcgattgaac ccggcccggt
    14761 ggaaggcatt cgcgccaccg gcgccaacaa gctcgaagag atcctctacg gcgtgctgcc
    14821 gcaggtgatg ccgctgctga tctcctactc cctctatcgc ttcgaatcca acgtccgctc
    14881 ggcgaccgtc gtcggcatgg tcggtgcagg cgggatcggc gtcaccctgt gggaagcgat
    14941 tcgcggtttc cagttccaac aaacctgcgc cctgatggtg cttatcatcg tcacggtcag
    15001 cctgctggat ttcctctctc aacggttgcg taagcacttt atctgataag cgaggcattg
    15061 atatctatgc acttgtctac acatccgacc agctacccaa cacgctatca ggagatagcc
    15121 gcaaaacttg agcaggagct tcgtcaacac taccgctgcg gcgactatct tcccgccgag
    15181 cagcaactgg cggcgcgctt tgaggtgaac cgccacaccc tgcgccgcgc catcgaccaa
    15241 ctggtggaaa aaggctgggt acagcgccgt cagggcgtcg gcgtgctggt gctgatgcgc
    15301 ccgttcgatt acccgctcaa cgcccaggcg cgttttagcc agaacctgct ggaccagggt
    15361 agccatccca ccagcgaaaa actgctgtcg gtattgcgcc ccgcgtccgg tcacgtcgct
    15421 gacgcgctgg ggattaccga gggcgagaac gttatccatt tgcgcaccct gcgtcgcgtc
    15481 aacggcgtcg cgctctgttt aatcgaccac tactttgcgg acctcaccct ttggccgacg
    15541 ctgcaacgct tcgacagcgg ttcgctgcac gattttctgc gcgagcaaac cggaattgcg
    15601 ctgcgccgca gccagacgcg gatcagcgcc cgtcgcgccc aggccaaaga gtgccagcgt
    15661 cttgaaattc cgaatatgtc gccgctgctg tgcgtgcgca cccttaacca ccgtgacggt
    15721 gaaagcagcc cggcggagta ctccgtcagc ctgacgcgcg ccgacatgat tgaattcact
    15781 atggagcact gaatgcacgc agataccgcg acccgccagc actggatgtc cgtactggcg
    15841 cacagccaac cggctgaact ggcagcccgc ctgaaagcac tgaacatcac cgccgactat
    15901 gaggttatcc gcgccgccga aaccggcctg gtgcagattc aggcgcggat gggcggcacc
    15961 ggcgaacgct tttttgccgg cgacgccacg ctcacccgcg ccgccgtacg cctgactgac
    16021 ggcacgctcg gctacagctg ggtgctgggg cgcgacaaac agcacgccga acgctgcgcg
    16081 ctgattgacg cgctgatgca gcaatctcgc tattttcaaa acttatcaga aacccttatt
    16141 gccccgctgg acgctgaccg tatggcacgc attgccgcac gccaggccga agtgaacgcc
    16201 agccgggtcg acttctttac gatggttcgc ggagacaacg catgaccctg gaaaccgctt
    16261 ttatgcttcc cgtgcaggat gcccagcaca gttttcgtcg cctgttaaag gccatgagcg
    16321 agccgggcgt gattgtcgcc ctgcatcagc tcaagcgcgg ctggcagccg ctgaatatcg
    16381 ccaccaccag cgtgctgctg acgctggccg ataacgacac gccggtgtgg cttgccgcgc
    16441 cattgagtaa cgatatcgtc agccagagcc tgcgttttca taccaacgcg ccgctggtca
    16501 gccagccgga acaggcgacc ttcgcggtga cggatgaggc gatttccagc gaacagctta
    16561 acgccctttc caccggcacc gccgttgcgc cggaagcggg tgcaacgctg attttacagg
    16621 tcgccagcct gagcggcggg cgcatgttac gtctcaccgg cgcgggcatt gccgaagaac
    16681 gaatgatcgc tccgcagctg ccggagtgca ttctgcacga actcaccgag cgcccgcacc
    16741 cgttcccgct cggcatcgac ctgatcctga cctgcggcga gcgcctgctg gctattccgc
    16801 gaaccactca tgtggaggtg tgctgatgta cgttgccgtg aaagggggcg agaaggcgat
    16861 cgacgccgct cacgccctgc aagagagccg acgccggggc gataccgatt tgcctgaact
    16921 gagcgtcgcc cagattgaac agcagcttaa tcttgcggtg gatcgcgtga tgaccgaagg
    16981 cggcattgcc gaccgcgaac tggcggcgct ggcgctgaaa caggccagcg gcgataacgt
    17041 cgaagcaatt ttcctgctgc gcgcctaccg caccacgctg gcgaagctcg cggtaagcga
    17101 gccgctcgac accacagaga tgcgtctcga acggcgtatt tcggcggttt ataaagacat
    17161 tcccggcggc cagctgcttg gcccaaccta cgactacacc catcgcctgc tcgatttcac
    17221 cctgctggca aacggtgaag cgccgacgct gaccaccgcc gacagcgaac aacagccgtc
    17281 gccgcacgtt ttcagcctgc tggcgcgtca ggggctggtg aagtttgaag aagatagcgg
    17341 cgcacagccg gatgacatca cccgcacgcc gccggtttac ccctgctcac gctcctcccg
    17401 cctgcaacaa ttaatgcgcg gggacgaagg ctatttgctg gcgctggcct actccaccca
    17461 gcgtggttac gggcgcaatc acccgttcgc gggcgagatc cgcagtggtt acatcgacgt
    17521 gtcgattgtg ccggaagagc tgggatttgc ggtaaacgtc ggcgaactac tgatgaccga
    17581 gtgtgaaatg gtcaacggtt ttatcgaccc gccggatgag ccgccgcact tcacgcgcgg
    17641 ctacgggctg gtattcggca tgagcgagcg caaagcgatg gcaatggcgc tggtcgatcg
    17701 tgcgttgcag gctccggaat acggcgagca cgcgacaggc ccggcgcagg atgaagagtt
    17761 tgtgctggca catgccgaca acgtcgaagc cgcaggcttt gtctcgcacc tcaaactccc
    17821 ccactacgtc gatttccagg ccgaactgga gctactcaaa cgtctgcaac aggagaagaa
    17881 ccatggctaa tctgagcggc tacaactttg cctacctcga cgagcagacc aaacgcatga
    17941 tccgccgcgc tatcttaaaa gcggtggcga tccccggtta tcaggtgccg tttggcgggc
    18001 gcgagatgcc gatgccatac ggctggggaa ccggcggcat acagctcacc gccagcgtga
    18061 ttggcgaaag cgacgtgctg aaggtgattg accagggtgc ggatgacacc accaacgccg
    18121 tgtcgattcg caacttcttt aagcgcgtga ccggggtaaa caccactgaa cgtacggacg
    18181 atgcgacgct tatccagacg cgtcaccgca tccccgaaac gccgctgacc gaagatcaga
    18241 tcattatctt ccaggtgcca atcccggaac cgctgcgctt tatcgagccg cgcgaaacgg
    18301 aaacccgcac catgcacgcg ctggaagagt acggcgtgat gcaggtgaaa ctgtatgaag
    18361 atatcgcccg cttcggtcat atcgccacta cctatgccta tccggtgaag gtgaacgggc
    18421 gctacgtaat ggacccgtcg ccgatcccga aattcgataa cccaaaaatg gacatgatgc
    18481 ccgccctgca actgttcggc gcggggcgcg agaagcgcat ctatgcggtg ccgccgttta
    18541 cccgcgtgga aagtctcgat ttcgacgatc acccgttcac cgttcagcag tgggatgagc
    18601 catgcgccat ctgcggatcg acccacagct atcttgatga agtggtgctg gatgacgccg
    18661 gaaaccgcat gtttgtctgc tccgataccg attattgccg ccaacagagc gaggcaaaaa
    18721 accaatgaat caaccgttac tttcggtcaa taacctgacc cacctttacg cgccgggcaa
    18781 aggctttagc gatgtctctt ttgatttatg gccgggggaa gtgctgggca ttgtcgggga
    18841 gtccggctcc gggaagacca cgctgctgaa atcaatctcc gcgcgcctga cgccgcagca
    18901 gggggagatt cactatgaga gccgttcgct gtacgcaatg ggcgaggccg accgccgtcg
    18961 cctgctgcgc accgaatggg gcgtagtgca tcagcatcca ctcgacggcc tgcgccgtca
    19021 ggtctcggca ggcggcaata tcggcgagcg gctgatggcg accggggcac gtcattacgg
    19081 cgatattcgt gccaccgcgc agaagtggct ggaagaggtg gaaattcccg ccaaccggat
    19141 cgacgacctg ccgaccacct tttccggcgg catgcagcaa cgtttgcaga tcgcccgcaa
    19201 cctggtgacg catccgaagc tggtgtttat ggacgagccg accggcggac tggatgtgtc
    19261 ggtacaggca cgcctgctcg acctgctgcg cggcctggtg gtggagctga acctcgcggt
    19321 agtgattgtc acccacgatt taggcgtcgc ccgcctgctg gcggaccgtt tgctggtgat
    19381 gaagcagggg caagtggtgg agagtgggtt aaccgaccgc gtgctcgacg acccgcacca
    19441 tccgtataca caactgctgg tgtcatcggt tttgcagaat tgagccggtg ccggatgcgg
    19501 cgtaaacgcc ttatccggcc tacaaatgcg ctccccgtag gtcggataag acgcgtcagc
    19561 gtcgcatccg acacccgaac cacgaggcga aaaatgatta acgtacaaaa cgtcagtaaa
    19621 accttcatcc tgcaccagca aaacggcgtg cgcctgcccg tcttacaacg tgcctcgctc
    19681 accgtcaacg cgggcgaatg cgtggtgctc cacggccatt ccggcagcgg caaatcaacc
    19741 ttgctgcgct cgctgtacgc caactatctg cccgacgaag ggcaaatcca gatcaaacac
    19801 ggtgacgaat gggtcgatct ggtcaccgcg ccagcgcgca aagtggtgga gatccgcaaa
    19861 accaccgtcg gctgggtgag ccagtttctg cgcgtcatcc cgcgtatttc agcactggaa
    19921 gtggtgatgc agccgctgct cgataccggc gttccgcgtg aagcctgcgc cgctaaagcc
    19981 gcgcgtctgc tcacccgcct gaacgtgccg gaacgcctgt ggcacctggc gccatcaacc
    20041 ttttccggtg gcgaacagca gcgcgtcaac atcgcccgcg gctttatcgt cgactacccc
    20101 attctgctgc tcgacgaacc taccgcctcg ctggacgcca aaaacagcgc cgccgtagtc
    20161 gagctgattc gcgaagccaa aacccgtggc gcggccatcg tcggcatctt ccatgacgaa
    20221 gccgtacgta atgacgtcgc cgaccgcttg cacccaatgg gagcctctgc atgattatca
    20281 ataacgtcaa actggtgctg gaaaacgaag tggtacacgg ttcgctggag gtgcaggatg
    20341 gcgaaatccg cgcctttgcc gaaagccaga gccgcctgcc agaagcgatg gacggcgaag
    20401 gtggctggct actacccggc ctgattgagc tgcataccga caacctcgat aaattcttca
    20461 ccccgcgccc gaaagtcgac tggcccgccc attcggcgat gagcagccac gacgcgctga
    20521 tggtggcaag cggcatcacc accgtactgg acgccgtggc gattggcgac gtgcgcgacg
    20581 gcggcgatcg gctggagaat ctggagaaga tgatcaacgc catcgaagag acgcaaaaac
    20641 gcggcgtcaa ccgcgccgag caccgcctgc acctgcgctg cgaactgccg catcacacca
    20701 cactgccgct gtttgaaaaa ctggttcagc gcgaaccggt gacgctggtg tcgctgatgg
    20761 accactcacc gggccagcgc cagttcgcta accgcgagaa gtatcgcgaa tattatcagg
    20821 gcaaatactc cctcaccgat gcgcagatgc agcagtacga agaagagcaa ttggcgctcg
    20881 ccgcacgctg gtcgcagccg aatcgcgaat ccatcgccgc cctgtgccac gcgcgacaaa
    20941 ttgcgctcgc cagccacgat gacgccacgc acgcccacgt tgccgaatcc caccaacttg
    21001 gcagcgtgat cgccgaattt cccaccacgt tcgaagcggc ggaagcctcg cgtaagcatg
    21061 gcatgaacgt gctgatgggt gcgccaaata tcgtgcgtgg cggctcgcac tccggcaatg
    21121 tggcggccag tgaactggcg cagcttggtc tgctggatat cctctcttcc gactactacc
    21181 ccgccagcct gctggatgcg gcgttccgcg tcgccgatga cgagagcaac cgctttacgc
    21241 taccgcaggc ggtgaggctg gtgaccaaaa atccggcgca ggcgctgaat cttcaggatc
    21301 gcggggtgat tggcgagggt aaacgcgctg acctggtgct ggcgcatcgc cagggcaatc
    21361 acattcatat cgaccacgtc tggcgtcagg gtaaaagggt gttctgatga tgggaaaact
    21421 gatttggtta atggggccgt ccggctccgg gaaagacagc ctgctggcgg aactccgcct
    21481 gcgggaacaa acgcagttac tggtggcgca tcgctacatc acgcgcgacg ccagcgccgg
    21541 aagtgaaaac catatcgccc tgagtgagcg ggagtttttt acccgcgcgg ggcaaaacct
    21601 gctggcctta agctggcacg ctaacggtct gtattatggc gtcggcatcg agattgacct
    21661 ctggctgcac gctggattcg acgtagtcgt caacggttca cgcgcgcatt tgccgcaggc
    21721 acgggcgcgc tatcaatcgg cgctgctgcc cgtctgttta caggtttcgc cggagatcct
    21781 gcgccagcgc ctggaaaacc gtggccgtga aaacgccagt gaaattaacg cccggctggc
    21841 gcgcgccgcc cgctatactc cacaggattg ccatacgctc aacaatgacg gcagcctgcg
    21901 ccagtcggtc gacacactgc tgacgctgat tcatcagaag gagaaacacc atgcctgctt
    21961 gtgagcttcg cccggccacg cagtacgata ccgacgcggt ttacgcgctg atttgtgagc
    22021 taaaacaggc ggagtttgac caccacgcgt ttcgcgtggg ctttaacgcc aacctgcgcg
    22081 acccgaacat gcgctaccat ctggcgctgc tggatggcga agttgtcggc atgatcggcc
    22141 tgcatttgca gtttcatctg catcatgtca actggatcgg cgaaattcag gaactggtgg
    22201 tgatgccgca ggcgcgcggc ctgaacgtcg gtagcaagtt gctggcgtgg gcagaagaag
    22261 aagcccgcca ggccggggcc gaaatgaccg agctttcgac caacgtgaag cgccacgacg
    22321 cgcaccgttt ctatctgcga gaaggctacg agcagagcca cttccgcttc accaaggcgc
    22381 tgtaacatga gcctgaccct cacgctcacc ggcactggcg gcgcacaggg cgttccggca
    22441 tggggctgcg agtgcgcggc ctgcgccaga gcgcggcgct cgccgcagta tcgccgccag
    22501 ccatgcagcg gcgtagtgaa gtttaacgac gcgatcaccc tgatcgacgc cgggcggcac
    22561 gatctcaccg atcgctggtc gcctggatcg ttccagcagt ttttgctgac gcactaccat
    22621 atggatcacg tccaggggct atttccgcta cgctggggcg tgggcgatgt gatcccggtc
    22681 tacggcccgc cggatgaaca gggctgcgac gatctgttta aacatccggg cctgcttgat
    22741 ttcagccaca cagtggaacc gtttgtggtg tttgatttgc aggggttgca ggtcacgccg
    22801 ctgccgctta accactcaaa actgaccttc ggttatctgc tggaaacggc acatagccgg
    22861 gtggcgtggc tgtctgacac cgctggcctg ccggaaaaaa cgctgaaatt tttactcaat
    22921 aatcatccgc aggtgatggt gattgattgc agtcacccgc cgcgcgcgga tgcaccgcgt
    22981 aatcactgtg atttaaatac cgtgcttgcg ctgaatcagg ttatccgctc gccacaggtg
    23041 attctgaccc atatcagcca ccagtttgat gcgtggctga tggaaaacgc actaccgtca
    23101 gggtttgagg tggggtttga tgggatggag attggggtgg cgtgatgaga gggaatgtgc
    23161 gcgctggccc cctcacccta accctctccc cagaggggcg aggggaccga ttgtgctcaa
    23221 tattgaatat tgcgctcgtt ttctccctct ccccattggg gtgaggggcg atgcctgctc
    23281 catacccaac ctcatcgccc atactcatct tccattctcc gctcttcatc ctccagttgc
    23341 cgacgctcct gatcaagctg gcgctggcga tcgtccagct gcctgcggcg atcttcaaac
    23401 tggcggcggc ggtcgtcata ttgtctgcgc cgatcgtcgc tcacttcacg ctgccagccg
    23461 tcgtcgcgcg aatcttcata gtctcgccca cggtcagggt tataagcgtc attaatcgcc
    23521 tgctgaatat taccaatagt atcgtcgata atatcagcct gggccggaac ggtggacagc
    23581 gtgagcaggg tgaataaaag aaatagcgga aagcgtttca ttagccaacc tcaaaaagaa
    23641 actctatcca cattagtcat tactcatcca tgcaagtagt ggatgaatct caatttctcc
    23701 gctgctctat tgccgtaatc gcctccacgc gttgttgatg acgaccgcct tcgtactgtg
    23761 cgcccagcca cgcatccaca atcatttttg ccagttcgag gccaaccact cgtgaaccaa
    23821 aagccagtgc aacttcactg gcataatgcg gataatcagt gcgctctgac gacctggtgt
    23881 ctttatcaat cacctcactc attgaccatg aataaaaggc tggcacagca acacagaatc
    23941 ctgcggtgcc agcgacatga ccgcccattg ctccggtacg ttttttaaaa agaaacaccg
    24001 gttgcattga taaattgtgc tcagtatttg ccgaagtaca atatatataa taaatatata
    24061 aaacacctca tattatttac agagataata aattcgcgta attttccccc ttcagcacaa
    24121 atgcctaata aaatggatcg ttccgctcac acttccactt attaaagtcg caaattccca
    24181 attatgataa cgccagcttt aaatgacgta ttctgcatgg attacatatt tacgacacaa
    24241 atgataaatg gagatttata tgaataactc tcggttattc cgtttgagca ggattgttat
    24301 tgcgttaact gctgccagcg gcatgatggt aaataccgct aacgcgaaag aggaagcgaa
    24361 agccgccact caatataccc aacaggttaa tcagaattac gccaaatcat taccgtttag
    24421 cgatcgtcag gattttgacg acgctcagcg tggatttatc gctccgctgc tggatgaagg
    24481 tattctgcgc gatgctaatg gcaaaccata ttatcgcggg gaagattata aatttgatat
    24541 caatgcgccc gcgcccgaaa ccgttaaccc tagcctgtgg cgtcagtcgc agttaaacgg
    24601 tatttccggc ctgtttaaag tcaccgacag aatgtaccag gttcgcggtc aggatatctc
    24661 caacatcacc tttatcgaag gtgataccgg gattattgtc atcgacccgc tggtgacgcc
    24721 accgagcgca aaagccgccc ttgaccttta cttccaaaat cgcccgcaaa aaccgattgt
    24781 tgcggttatt tatacccaca gccataccga ccactatggc ggcgtgaaag gcattatctc
    24841 tgaagctgat gtgaaatccg gtaaagtgca ggttatcgcc cctgctggct ttatggacga
    24901 agccatcagc gaaaacgtac tggcgggtaa tattatgagc cgccgtgcac tttactccta
    24961 cggcctgctg ctggcgcata accctcaggg taacatcggc aatggtcttg gcgtaacgct
    25021 ggcatcgggc tacccgagca tcatcgcacc gaacaaaacc atcaccaaaa ccggtgagaa
    25081 gatgattatc gacggcctgg agtttgactt cctgatgacc ccaggtagcg aagcaccagc
    25141 cgaaatgcac ttctatattc cggccctgaa agcgctgtgt accgccgaga acgccacgca
    25201 taccctgcac aacttctaca ctctacgcgg cgcgaaaacc cgcgacacca gcaagtggac
    25261 cgagtatctg aacgaaacgc tggatatgtg gggtaacgac gcggaagtcc tgtttatgcc
    25321 gcacacctgg ccggtctggg gcaataagca tatcaatgat tatattggta aatatcgcga
    25381 tactatcaag tacattcacg accagaccct gcacctggcg aaccagggct acaccatgaa
    25441 tgaaatcggc gacatgatta aactgccgcc tgcacttgcc aataactggg ccagccgtgg
    25501 ctattacggt tctgtcagcc acaacgcccg cgcggtgtat aacttctatc ttggctatta
    25561 cgacggtaac ccggctaacc tgcatccgta tggtcaggtg gagatgggta aacgttacgt
    25621 gcaggcgctg ggcggttctg cccgtgtcat caacctggcg caagaagcga acaagcaagg
    25681 tgattaccgc tggtcggcag aactgctgaa acaggtgatt gccgccaacc cgggtgacca
    25741 ggtcgcgaag aatctgcaag cgaataactt tgaacagctg ggctatcagg ccgagtccgc
    25801 cacctggcgc ggtttctacc tgaccggcgc gaaagagctg cgcgaagggg tgcataagtt
    25861 cagccacggc accaccggtt ccccggacac cattcgcggg atgtcggtcg aaatgctgtt
    25921 cgactttatg tccgttcgcc tcgatagcgc gaaagccgcg ggtaaaaata tcagcctgaa
    25981 cttcaatatg agcaatggcg ataacctcaa cctgacgctg aacgatagcg tgcttaacta
    26041 ccgtaaaaca ctgcaatccc aagctgacgc ctctttctac atcagccgtg aagatctgca
    26101 cgccgtgctg accgggcaag ccaaaatggc ggatctggta aaagcgaaga aagccaaaat
    26161 tattggcaat ggcgcgaaac tggaagaaat tatcgcctgt ctggataatt tcgatttgtg
    26221 ggtgaatatc gtaaccccaa attaatgtca ctcgcttttg g
//
LOCUS       DAZGJW010000062        26138 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZGJW010000062 DAZGJW010000000
VERSION     DAZGJW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.062"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="ACHKIK_002536"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="ACHKIK_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO9083692.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="ACHKIK_002537"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="ACHKIK_002537"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO9083693.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="ACHKIK_002538"
     CDS             complement(941..1408)
                     /locus_tag="ACHKIK_002538"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO9083694.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHKIK_002539"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHKIK_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO9083695.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="ACHKIK_002540"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="ACHKIK_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO9083696.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="ACHKIK_002541"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="ACHKIK_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO9083697.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="ACHKIK_002542"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="ACHKIK_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO9083698.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="ACHKIK_002543"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="ACHKIK_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO9083699.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHKIK_002544"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHKIK_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO9083700.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="ACHKIK_002545"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="ACHKIK_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO9083701.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHKIK_002546"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHKIK_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO9083702.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="ACHKIK_002547"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="ACHKIK_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO9083703.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHKIK_002548"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHKIK_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO9083704.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHKIK_002549"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHKIK_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO9083705.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="ACHKIK_002550"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="ACHKIK_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO9083706.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="ACHKIK_002551"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="ACHKIK_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO9083707.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="ACHKIK_002552"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="ACHKIK_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO9083708.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHKIK_002553"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHKIK_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO9083709.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHKIK_002554"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHKIK_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO9083710.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="ACHKIK_002555"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="ACHKIK_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO9083711.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="ACHKIK_002556"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="ACHKIK_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO9083712.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHKIK_002557"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHKIK_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO9083713.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHKIK_002558"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHKIK_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO9083714.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="ACHKIK_002559"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="ACHKIK_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO9083715.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="ACHKIK_002560"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="ACHKIK_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO9083716.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHKIK_002561"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHKIK_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO9083717.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHKIK_002562"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHKIK_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO9083718.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHKIK_002563"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHKIK_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO9083719.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHKIK_002564"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHKIK_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO9083720.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHKIK_002565"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHKIK_002565"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO9083721.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHKIK_002566"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHKIK_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO9083722.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15548..15916
                     /locus_tag="ACHKIK_002567"
     CDS             15548..15916
                     /locus_tag="ACHKIK_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO9083723.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15927..16352
                     /gene="zntR"
                     /locus_tag="ACHKIK_002568"
     CDS             15927..16352
                     /gene="zntR"
                     /locus_tag="ACHKIK_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO9083724.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16408..16626
                     /gene="arfA"
                     /locus_tag="ACHKIK_002569"
     CDS             16408..16626
                     /gene="arfA"
                     /locus_tag="ACHKIK_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO9083725.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="ACHKIK_002570"
     CDS             complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="ACHKIK_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO9083726.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="ACHKIK_002571"
     CDS             complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="ACHKIK_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO9083727.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="ACHKIK_002572"
     CDS             complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="ACHKIK_002572"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO9083728.1"
                     /translation="MKKQRNLRSMAAQAVEQIVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGVYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQEELLAGFYASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGTEEGDGFFYAKLIKK"
     gene            complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="ACHKIK_002573"
     CDS             complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="ACHKIK_002573"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO9083729.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20858..21367)
                     /gene="def"
                     /locus_tag="ACHKIK_002574"
     CDS             complement(20858..21367)
                     /gene="def"
                     /locus_tag="ACHKIK_002574"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO9083730.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21497..22621
                     /gene="dprA"
                     /locus_tag="ACHKIK_002575"
     CDS             21497..22621
                     /gene="dprA"
                     /locus_tag="ACHKIK_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO9083731.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22593..23067
                     /gene="smg"
                     /locus_tag="ACHKIK_002576"
                     /pseudo
     CDS             22593..23067
                     /gene="smg"
                     /locus_tag="ACHKIK_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
     gene            23096..23638
                     /locus_tag="ACHKIK_002577"
     CDS             23096..23638
                     /locus_tag="ACHKIK_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO9083732.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCLACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23643..24215
                     /gene="tsaC"
                     /locus_tag="ACHKIK_002578"
     CDS             23643..24215
                     /gene="tsaC"
                     /locus_tag="ACHKIK_002578"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO9083733.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24220..25038
                     /gene="aroE"
                     /locus_tag="ACHKIK_002579"
     CDS             24220..25038
                     /gene="aroE"
                     /locus_tag="ACHKIK_002579"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO9083734.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25035..25292
                     /locus_tag="ACHKIK_002580"
     CDS             25035..25292
                     /locus_tag="ACHKIK_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO9083735.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25268..25822)
                     /locus_tag="ACHKIK_002581"
     CDS             complement(25268..25822)
                     /locus_tag="ACHKIK_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO9083736.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6723 a         6202 c         6776 g         6437 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagcgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ccgatgaaga aggtcatatt gactggctcg aaactgagct ggatctcatc ggtaaaatcg
      901 gtttacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gctagcaaac
      961 tatcccaacc tacaacgaaa cccgcagccg ccagaaatgg cccaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc atgagcagac caacaactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccaggtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgacg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaaccgt
     1261 caggattaca aacctgagag aacaaaagac ctgaccagag taacgggcag gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccata aaaaaagtag gacagataaa caagcgtaca
     1381 aaatcagaaa gggaaggtta gcgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gttccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttctggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttataaa ggcaagggtg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactctg caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaaatctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggta gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccaggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag attactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cctggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacctg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacaacgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agcatcccca cttatctaca atagctgtac tctttttgtt catcccctgg
    15541 agtatttatg tggttacttg accagtgggc agagcgtcat atagcagaag cgcaagcgaa
    15601 aggtgagttt gataacctgg caggtagcgg cgaaccattg atactggatg atgattctca
    15661 tgtgccaccg gaattacgtg cggggtatcg cttgctgaag aatgccggtt gcttaccgcc
    15721 agaacttgag cagcggaggg aagcaattca gcttctggat atcctgagag gtatccgtca
    15781 cgatgatccg caatatcaag aggttagccg tcgattgtca ttactggaat tgaagctgcg
    15841 acaagctgga ttgagtaccg attttttacg cggcgattat gctgacaagt tgttggacaa
    15901 aatcaacgat aactagtgga gtatgtatgt atcgcattgg tgagctggca aaaatggcgg
    15961 aagtaacacc tgacacgatt cgttattacg aaaaacagca gatgatggag catgaagtgc
    16021 gtactgaagg cgggtttcgc ctgtataccg aaagcgatct ccagcgattg aaatttatcc
    16081 gccatgccag acaactaggt ttcagtctgg agtcgatccg cgagttgctg tcgatccgca
    16141 tcgatcctga acaccatacc tgtcaggagt caaaaggcat tgtgcaggaa agattgcagg
    16201 aagtcgaagc acggatagcc gagttgcaga gtatgcagcg ttccttgcaa cgccttaacg
    16261 atgcctgttg tgggaccgcc catagcagtg tttattgttc gattcttgaa gctcttgaac
    16321 aaggggcgag tggcgttaag agtggctgtt gattttttgc actgacagga ttacactcgc
    16381 gccgtaaaat aaccaactgg agtttttatg agccgatatc agcatactaa agggcagata
    16441 aaggataatg cgatagaagc attactacat gatcccttat tccgacagcg agtagagaaa
    16501 aataagaaag ggaaaggcag ttacatgcga aaaggaaaac atggcaatcg ggggaactgg
    16561 gaggccagtg gcaagaaagt aaatcacttt tttaccactg gtcttctgct ttcaggcgct
    16621 tgttaagagc ggttattctg ctctttcagc aaatcacgaa tttctgccag taatacttct
    16681 tctttagttg gtgcaggtgc ggcagctggt tcttcttttt tccgattcag tttgttgatt
    16741 agcttaatcg ccataaagat ggcaaaggcc acaatcagaa aatcaaagac gttttgaatg
    16801 aagacaccgt aatgcatcac aacagcaggg atatccccct gcgcatcgcg tagcgtgaca
    16861 gcaaactgtt taaaatcgat cccgccaatt aataagccca gaggaggcat gatgatatcg
    16921 gcaaccagtg aagagacaat cttcccgaat gccgcaccga taatgacacc caccgccaaa
    16981 tccaccacgt tcccgcgcat cgcaaattcg cgaaattctt taataatgct catgttattc
    17041 tccctatgaa gccgacaacc ataagtctaa caaatgttaa gccattttcc tgccaggaat
    17101 gaatttaatt aatccttgat aatcaaaggg aaataataag gcgtcattag acgccttatt
    17161 aattacaaga agaaagggct tggttggaag agtctttcga cgtcggtaat aaacttttta
    17221 tctgtgagga acataattac gtgatcgcct tgctcaatgc gcagattgtc attggcaatc
    17281 atcacgtcgt ttccacgtac caccgctcca ataatcgttc ctggcggtag cttgatttcg
    17341 tcaatgactc tgccgacaac gcgtgaggtg ctttcatcac cgtgagcaac ggcttcaata
    17401 gcttctgcta cgccgcggcg caatgaggaa acaccaacaa tatctgcttt tcgcacatgg
    17461 ctaagcaacg cagaaatagt tgcttgttgt ggtgaaatcg caatatcgat cacactcccc
    17521 tgaaccagat ccacataagc gcgacgctgg atcaatacca tcaccttttt cgcacccata
    17581 cgtttggcaa gcatggcgga catgatattg gcctcgtcat cgttggtgac ggcaataaac
    17641 agatcaactt gatcgatatg ttcttcggcc agcagttctt gatccgacgc atcaccaaaa
    17701 aagacgatcg tattctgcaa cttttccgcc agttcggcag cgcgctgctg attacgttcg
    17761 atgagtttaa cgctgtaatc tttttccaga cgacgcgcca gccctgcacc gatattaccg
    17821 ccaccaacca gcatgatccg cttatacggt ttttccagtc gctgtaattc actcatcacc
    17881 gcacggatat gctgtgaagc ggcaataaag aacacttcat caccagcttc cacaatggtc
    17941 gaaccttgtg gacgaatggg gcgatcgtgg cggaaaattg ctgccacacg agtatcgata
    18001 tgtggcatat gttcgcgcat ggtcgaaagt gcgttaccaa tcagcgggcc gccataatag
    18061 gctttaacca cagcgaggct gactttaccc tcagcgaagt tcaccacctg caatgcgccg
    18121 ggatactcaa tcagtcggta aatattatcg ataaccaact gctctggtgc gatcagatga
    18181 tcaatcggta cagcatctga atgaaatagc ttatcggcat cgcgcacgta gtctggtgag
    18241 cggatgcgag cgatgcgatt aggggtgttg aaaagtgagt aggctacctg gcaggcaacc
    18301 atattggttt catctgaact ggttacagca accagcatat cggcgtcgtc ggcacctgcc
    18361 tcccgcaata cgcgtggatg agagccatgc ccctgcacga ctcgcaggtc aaatttatcc
    18421 tgcaaggtcc gcagacgctc accgttggta tcgacaacag tgatatcgtt gttctcgcca
    18481 accaggtttt ccgccagtgt gccgccaacc tggccggcac ccagaatgat aattttcatc
    18541 agtcgcgacc cgttatctca tcactttttg attagcttag cgtaaaagaa gccgtcgccc
    18601 tcttcggtac caggcagatt ttgtttaccc ggttgctctg gtgttcctgt ttcgcaaagt
    18661 tcggcatcag cggtacgttg caaaaaggct ttaatctgca ggctattctc ttccggtaac
    18721 accgaacagg tggcatagac cagggttcca ccggatttta aatgcggcca aatggcgtcg
    18781 agaatttcag actgcaattg cgcgagttcc gggatatcgc gatcgcgacg taaccattta
    18841 atatccggat ggcgacgaat cacaccggtt gctgaacaag gcgcatctaa taaaatgcga
    18901 tcaaactgtt gctcgccaca ccattgggaa gggtagcggc catcaccttg tttcacggtc
    18961 gctttcatac caaggcgttt taaattgtcg taaacgcgag agaggcgctg ttcgtcaata
    19021 tcaaccgcaa caacctgcgc ttctggtgcc acctcaagga tatgcgttgt tttaccgccg
    19081 ggggcggcac aaagatccag aatgtgttca ccgttttgtg gcgcaagcca ggtcatgcaa
    19141 ccttgtgctg atgcatcctg aacggtaacc catccgtctt caaaaccagg tagcgcatga
    19201 acaggtgcag gtgtttccag ccgtacagca tcagggtaat ccgcatgcgg gaaacctttc
    19261 attcctgctt catccaacaa tgcaagccag ctgtcgcggg aatgatgcgt acgattaacg
    19321 cgcagccaca ttggcggacg ctggttatta gcttcgacga tggattgcca ctgctctgga
    19381 tacgcttttt gcagacgctt cagcaaccag gaaggatgca gataacgtgc atcactggca
    19441 taaaacccgg ctaataactc ttcttgctga cgctggaact ggcgtaatac accgttaatc
    19501 agccccttaa gttgcgggcg cttaattgcg acagcgcctt caaccgtttc agccagcgca
    19561 gcatgaggtg gaatgcgggt ataaagcagt tgatacacac caaccataat caggtaatgc
    19621 acggtccgct gtttgccggt catcggacgg gccattaact tattaatcag ccagtctaac
    19681 tgtgaaagcg tacgcagtac gccaaagcac aactcttgaa gaagtgcttt gtctttatcg
    19741 gaaacttttt gctggagcgg tggcagaatg ttgcttaatg attgcccttg ctcgacgatt
    19801 tgttcaacgg cctgtgccgc catgctacgt aaattacgtt gttttttcat aagtataaaa
    19861 atgcccggta agaccgggct tagaagagtg gactatcaga ccagacggtt gcccggaaca
    19921 aaccattccc gacgagaatt caggaggtct tgcgcgctca tcgctttctt acccgcaggt
    19981 tgtaacgaga gcaggttcag gatgccatca ccagtcgcaa cctgaatgcc ttgtttattg
    20041 gcttcaagga tcgttcctgg tgcagcgttg gttgccgtat caatgaccga cgctttccag
    20101 actttaaccg gttgtccttc aatttccagc cagctcattg gccatgggtt gaaagcgcga
    20161 atgcagcgtt caagctgcgc tgccgaaagc gaccagtcaa tacgcgcttc ttctttactc
    20221 aacttctcgg cgtaagtgac aagagtttcg tcctgaactt ctggtttcgc cgtgccgtct
    20281 gctagttgtt tcaacgtggt gataagccct tgtgggccaa gctctgccag cttgtcgtac
    20341 agcgtaccac tggtatcttc tgcagtaatc gggcaggaga gcttataaag catgtcaccg
    20401 gtgtctaaac caacatccat ttgcataatg gtcacaccag tttctgcatc acccgcccat
    20461 agtgagcgtt ggattggtgc agcaccgcgc cagcgtggca gcagtgaacc atgaacgttg
    20521 atacagccaa gacgcggcat ctccagcact gctttaggca gaattaagcc ataggcgacg
    20581 acgaccataa catcagcctg cagatcggcg accagttgct ggctttcttg tggacgcagg
    20641 gaaacaggtt gaaaaacggg cagacctttt tcctcagcca gaactttaac cgggctgggc
    20701 atcagttttt taccgcgtcc tgccggtcgg tctggctggg tgaacacgcc aacgacgtta
    20761 tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa agtcaggtgt acccgcaaaa
    20821 ataatacgta gtgattctga cacgttagtt cttatcctta agcccgggct ttcagacgat
    20881 ctagtttttc aactttctga cgaatacgtt gttgtttcag cggtgacaga taatccataa
    20941 acagtttgcc gaccaggtga tccatctcat gctgaataca gatggctaac agcccctctg
    21001 cttccagttc aaatggttta ccgtcgcggt caagggcgcg aattttaact ttctctgcgc
    21061 gcggcactaa agcacgttgt tcagggatcg acaggcaacc ttcttcaatg cctgtttcgc
    21121 cgcttttttc taaaagctcc ggattgatta acaccagccg ttcgtcacgg ttttccgaaa
    21181 catcaataac aatgatacgt tgatggatat caacctgggt tgccgccagg ccaatacctt
    21241 cttctgcgta catcgtctcg aacatatcat cgacgatacg ctgtatttct gcattcactt
    21301 cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg aatatgtaac acttgcaaaa
    21361 ctgacataaa tctccagaga tgtgttcagg agttagaaag attatttctt ctattctaga
    21421 caaatccccc tctgattgac agcatcactg accaatcgca aagattgcta aggctgctta
    21481 tggcagggag ataaggatgg tcgatacaga aatttggctg cgtttaatga gtatcagcag
    21541 cttgtacggc gatgatatgg tccgtatagc tcactggctg gcaaaacagt cgcaaattga
    21601 tgcggttgga ttgcagcaaa cagggcttac attgcggcag gcacaacgct ttctttcatt
    21661 tccgcgaaag agtatcgaaa gctcactttg ttggttggag caacccaacc atcatttaat
    21721 ccctgcggac agcgaatttt atcctcctca acttctggcg acgacagatt accccggcgc
    21781 actgtttgtt gaaggagaac tgcacgcgct gcattcattt cagcttgccg tagtggggag
    21841 tcgggcgcat tcatggtatg gcgagcgatg gggacggtta ttttgcgaaa ctctggcgac
    21901 gcgtggagtg acaattacga gtggactggc gcgtggaatc gatggtgtgg cgcataaagc
    21961 ggccttacag gtaaatggcg tcagcattgc tgtattgggg aatggactta ataccattca
    22021 tccccgccgc catgcccgac tggctgccag tctgcttgaa catggcggag ctctcgtctc
    22081 ggaatttccc ctcgatgttc caccccttgc ttacaatttc ccacgaagaa atcgcattat
    22141 cagtggtcta agtaaaggtg tactggtggt ggaagcggct ttgcgcagtg gttcgctggt
    22201 gacagcacgt tgtgcgcttg agcaggggcg tgaagttttt gccttgccag gtccaatagg
    22261 gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga
    22321 accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga
    22381 aaattcattt tattcaccag atcagcaaga cgtggcattg ccatttcctg agctcctggc
    22441 taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt
    22501 gccagaggta gttactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc
    22561 cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg
    22621 aaacctatat tcacacagaa gctgagttgc gtgtggatca agacaaactt gaacaggatc
    22681 ttaccgacgc aggatttgat cgagaagata tctacaatgc cctgctttgg ctggaaaagc
    22741 ttgcggatta tcaggaagga ctggcagaac cgatgcaact ggcctctgat ccactctcca
    22801 atgcgtattt atacccctga ggagtgtgaa agactggatg ccagctgtcg tggttttctg
    22861 cttttccttg agcaaattca ggtgcttaac cttgaaaccc gtgaaatggt gatagagcga
    22921 gtgctcgcgc tggataccgc agagttcgac ctggaagacc tgaaatgggt aatcctgatg
    22981 gtgttgttca atattccggg ctgcgaaaat gcgtaccagc aaatggaaga attactcttt
    23041 gaagtgaatg aaggtatgct gcattaatca tttctttaat tcagcataag ttgttatggc
    23101 gaaatcagca ctgttcacgg tgcgtaataa tgagtcctgc ccaaagtgcg gggctgaact
    23161 ggttattcga tccgggaaac acggtccgtt tcttggatgc tcacagtatc cggcgtgtga
    23221 ctacgtccgt cctctgaaat cttcagcgga tggacatatt gtcaaagttc tggaggggca
    23281 ggtttgcctt gcatgtggcg caaatctggt attacgccag ggacgctttg gtatgtttat
    23341 tggttgcagt aactaccctg aatgcgaaca taccgaactt atcgataaac cggacgaaac
    23401 agcaattaca tgcccccaat gccggacggg ccatctggtc cagcgccgct cccgttatgg
    23461 caaaacattt cactcctgtg atcgctaccc ggagtgtcaa tttgccatta acttcaaacc
    23521 catagctgga gaatgccctg agtgtcatta tccactactc atcgaaaaga aaaccgcgca
    23581 gggtgtaaaa cacttttgtg ccagtaaaca atgtggaaag ccggtttcgg cggaataata
    23641 acgtgaataa taacctgcaa ggagacgcta tcgcagctgc gatagatgtc ctcaatgaag
    23701 aacgtgtcat cgcctatcca acggaagccg ttttcggtgt tgggtgcgat cctgatagcg
    23761 aaacagcagt gatgagactg ttggagttaa aacagcgtcc ggttgataag gggctgattt
    23821 taatcgcggc aaattacgag cagcttaaac cctatattga tgacaccatg ctgactgatg
    23881 cgcagcgtga aaccattttt tcccgctggc caggtcctgt cacctttgtc tttcccgcgc
    23941 ctgcgacaac accgcgctgg ttgacgggcc gctttgattc gcttgctgta cgagtcaccg
    24001 accatccgtt ggtggttgct ttgtgccagg cttatggtaa accgctggtt tctaccagtg
    24061 ccaacttgag tggattgcca ccttgtcgaa cagtagacga agttcgcgca caatttggcg
    24121 cggcgttccc ggttgtgcct ggtgaaacgg gggggcgttt aaatccttca gaaatccgcg
    24181 atgccctgac gggtgaactg tttcgacagg ggtaacataa tggaaaccta tgctgttttt
    24241 ggtaatccga tagcccacag caaatcgcca ttcattcatc agcaatttgc tcagcaactg
    24301 aatattgaac atccctatgg gcgcgtgttg gcacccatca atgatttcat caacacactg
    24361 aacgctttct ttcgtgctgg tggtaaaggt gcgaatgtga cggtgccttt taaagaagag
    24421 gcttttgcca gagcggatga gcttactgaa cgggcagcgt tggctggtgc tgttaatacc
    24481 ctcaagcggt tagaagatgg acgcctgctg ggtgacaata ccgatggtat aggtttgtta
    24541 agcgatctgg aacgtctgtc ttttatccgt cctggtttac gtattctgct catcggcgct
    24601 ggtggagcat ctcgcggcgt actactgcca cttctttccc tggactgtgc ggtgacaatc
    24661 actaatcgga cggtatcccg cgcggaagag ttggctaaat tgtttgcgca cactggcagt
    24721 attcaggcgt tgggtatgga cgaactggaa ggtcatgagt ttgatctcat tattaatgca
    24781 acatccagtg gcatcagtgg tgatattccg gcgatcccgt catcgctcat tcatccaggc
    24841 atttattgct atgacatgtt ctatcagaaa ggaaaaactc cttttctggc atggtgtgag
    24901 cagcgaggct caaagcgtaa tgctgatggt ttaggaatgc tggtggcaca ggcggctcat
    24961 gcctttcttc tctggcacgg tgttctgcct gacgtagaac cagttataaa gcaattgcag
    25021 gaggaattgt ccgcgtgaat caggccatcc agtttccgga cagagaagag tgggacgaga
    25081 ataaaaaatg tgtatgtttt cccgctctcg tgaatggcat gcaactgaca tgtgcgatct
    25141 ctggcgagag tctggcgtat cgttttactg gagatacgcc agaacagtgg ttagcgagtt
    25201 ttcgtcagca tcgctgggat ctggaagaag aagcggaaaa cttaattcag gaacaaagtg
    25261 aagatgatca aggctgggtc tggttaccct gatccagata ttcgtccttc catttcacgt
    25321 aattattcgc ggaatagcgt aacccagcct tctcttcatc acttaacggg cggatctgtt
    25381 tgacggggct accgagatac agatatccgc tctccagccg tttattttgt gggaccagac
    25441 tacccgcacc aatcatcaca tcatcttcta ctattgcgcc atcaagtaaa attgagccca
    25501 tcccaaccaa aactcgattg ccaatggtgc agccgtggag catcaccttg tgaccaacag
    25561 tgacatcttc gccaatggtt aatgggttgc catctgggtt gtacgaggat ttatgagtga
    25621 catgcaacat actgccatcc tggatattgg tgcgtgctcc gatctgtaca taatgtacat
    25681 ctccacgaat cacaacgagc ggccagatcc ccacatcatc agccagacga acgtcaccaa
    25741 tcacgacact gctatcgtcg atcattacgc gctgaccgat ttgtggaaaa agatcgcggt
    25801 atgggcgtaa aacatcagac atacttacct cagcaataaa tgatttacta atgactttgg
    25861 gggcattatt ggccttgtgc aagtctttta gtatgcaaaa aagcaccgtt ttgtgtgcga
    25921 atgcagcaaa aagggtgaaa aaacaacaaa cagaaaaaaa gatcaaaaaa gtacttgtgc
    25981 aaaaaattgg gatccctata atgcgcctcc gttgagacga caacgtgaaa cacttcacaa
    26041 gatggtcgta acaacgaaga gaaaaaatcc tgaaattaag ggttgactct gaaagaggaa
    26101 agcgtaatat acgccacctc gcgacagtga gctgaaag
//
LOCUS       DAZGJW010000063        25592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZGJW010000063 DAZGJW010000000
VERSION     DAZGJW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.063"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            270..689
                     /locus_tag="ACHKIK_002582"
     CDS             270..689
                     /locus_tag="ACHKIK_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO9083737.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            689..1956
                     /gene="umuC"
                     /locus_tag="ACHKIK_002583"
                     /pseudo
     CDS             689..1956
                     /gene="umuC"
                     /locus_tag="ACHKIK_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(2002..2532)
                     /gene="dsbB"
                     /locus_tag="ACHKIK_002584"
     CDS             complement(2002..2532)
                     /gene="dsbB"
                     /locus_tag="ACHKIK_002584"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO9083738.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2678..4219)
                     /gene="nhaB"
                     /locus_tag="ACHKIK_002585"
     CDS             complement(2678..4219)
                     /gene="nhaB"
                     /locus_tag="ACHKIK_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO9083739.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4441..5160
                     /gene="fadR"
                     /locus_tag="ACHKIK_002586"
     CDS             4441..5160
                     /gene="fadR"
                     /locus_tag="ACHKIK_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO9083740.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5212..6744)
                     /locus_tag="ACHKIK_002587"
     CDS             complement(5212..6744)
                     /locus_tag="ACHKIK_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO9083741.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7074..8372
                     /gene="dadA"
                     /locus_tag="ACHKIK_002588"
     CDS             7074..8372
                     /gene="dadA"
                     /locus_tag="ACHKIK_002588"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO9083742.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVHPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8382..9452
                     /gene="dadX"
                     /locus_tag="ACHKIK_002589"
     CDS             8382..9452
                     /gene="dadX"
                     /locus_tag="ACHKIK_002589"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO9083743.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9838..11574)
                     /locus_tag="ACHKIK_002590"
     CDS             complement(9838..11574)
                     /locus_tag="ACHKIK_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO9083744.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11669..12583)
                     /gene="ldcA"
                     /locus_tag="ACHKIK_002591"
     CDS             complement(11669..12583)
                     /gene="ldcA"
                     /locus_tag="ACHKIK_002591"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO9083745.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12683..13294
                     /gene="emtA"
                     /locus_tag="ACHKIK_002592"
     CDS             12683..13294
                     /gene="emtA"
                     /locus_tag="ACHKIK_002592"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO9083746.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13296..14030)
                     /locus_tag="ACHKIK_002593"
     CDS             complement(13296..14030)
                     /locus_tag="ACHKIK_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO9083747.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14231..14485
                     /locus_tag="ACHKIK_002594"
     CDS             14231..14485
                     /locus_tag="ACHKIK_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO9083748.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            14663..15103
                     /gene="ycgY"
                     /locus_tag="ACHKIK_002595"
     CDS             14663..15103
                     /gene="ycgY"
                     /locus_tag="ACHKIK_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFO9083749.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLAPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15182..16879)
                     /gene="treA"
                     /locus_tag="ACHKIK_002596"
     CDS             complement(15182..16879)
                     /gene="treA"
                     /locus_tag="ACHKIK_002596"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO9083750.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17199..18617)
                     /gene="dhaM"
                     /locus_tag="ACHKIK_002597"
     CDS             complement(17199..18617)
                     /gene="dhaM"
                     /locus_tag="ACHKIK_002597"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO9083751.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(18628..19260)
                     /gene="dhaL"
                     /locus_tag="ACHKIK_002598"
     CDS             complement(18628..19260)
                     /gene="dhaL"
                     /locus_tag="ACHKIK_002598"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO9083752.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19271..20341)
                     /gene="dhaK"
                     /locus_tag="ACHKIK_002599"
     CDS             complement(19271..20341)
                     /gene="dhaK"
                     /locus_tag="ACHKIK_002599"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO9083753.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20569..22488
                     /gene="dhaR"
                     /locus_tag="ACHKIK_002600"
     CDS             20569..22488
                     /gene="dhaR"
                     /locus_tag="ACHKIK_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO9083754.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22588..25473)
                     /locus_tag="ACHKIK_002601"
     CDS             complement(22588..25473)
                     /locus_tag="ACHKIK_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO9083755.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINEGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQSNLFDQKQVNGGYRFSF"
BASE COUNT         6319 a         6355 c         6596 g         6322 t
ORIGIN      
        1 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
       61 gaatgcttct attctatttt ttagccgggt aatatttttc atttctgctg gatgagcgta
      121 gtcgccagaa ggccgcgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
      181 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac tactgtatat
      241 aaaaacagta tagcttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
      301 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
      361 cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga
      421 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
      481 tactgattgt cgatagcgct attaccgcca gccatggtga tattgtcatc gctgctgttg
      541 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
      601 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
      661 tgatccacgt cgttaaggcg atgcgctgat gtttgctctc tgtgatgtaa acgcgtttta
      721 tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc
      781 gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gtgttaaaat
      841 gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag
      901 cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact
      961 atcgccccgc gtcgagattt acagtattga tgaggcattc tgcgatctga caggtgtgcg
     1021 taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac
     1081 ccatcttact gttggtgtgg ggatcgccca gaccaaaacg ctggctaagc ttgccaatca
     1141 tgcggcaaaa aaatggcagc ggcagacggg ggggtggtgg atttatcaaa tctggaacgc
     1201 cagcgtaaat taatgtctgc tctccccgtg gatgaagtct gggggattgg acggcggatc
     1261 agcaaaaaac tggacgcgat ggggatcaaa accgttcttg atttggcgga tacagatatc
     1321 cggtttatcc gtaaacattt taatgtcgtg ctcgaaagaa cggtgcgtga actgcgcggc
     1381 gaaccctgtt tgcaactgga agagtttgca ccgacgaagc aggaaattat ctgttcccgc
     1441 tcgtttggtg aacgcatcac ggattatcca tcgatgcggc aggccatttg tagttacgct
     1501 gcccgggcgg cggaaaaact tcgcagcgag catcaatatt gtcggtttat ctccacattt
     1561 attaagacgt caccatttgc gctcaatgaa ccttattacg gcaatagcgc gtcggtaaaa
     1621 ctgctgacgc ccactcagga cagcagggat atcattaacg ctgctacgcg atctctggat
     1681 gccatctggc aagcgggcca tcgctaccaa aaagcgggcg tgatgctggg ggatttcttc
     1741 agtcagggag tcgcgcagct caatttattc gatgacaacg caccgcgccc cgggagtgag
     1801 caattgatga cggtaatgga tacactgaat gctaaagagg gcagaggaac actctatttt
     1861 gccgggcagg ggatccagca acaatggcag atgaagcgtg ccatgctttc accacgttat
     1921 acaacgcgaa gttctgattt actgcgggtc aaataaatat agcggcagga aaaaagcgct
     1981 cccgcaggag cgccgaatgg attagcgacc gaacaggtca cgttttttcg ctttaaacgg
     2041 ctgggaaatc accaccagca ccgcgacaat caggtaagcg ataaaaatac cgagcagcca
     2101 ctgcggcatt tccataccta aaaaatccca ctgacgctcg gcgcaatcgc cagaggcgac
     2161 aaacacttgc ggcacccatt tatccagcgg cagccattcc gggaaacgaa ccataaaatc
     2221 acaggtggca aacggcgaag gatagagctg aagcatggtg tgctcgtaag ttaactgcac
     2281 accgcggaac gcactataca accagataac catcgctaca taacgcagcg gagttttcgg
     2341 ggcgatcgcg ccaatcagcg cagcacccag aacgccgaat aacgcgcagc gttcataaat
     2401 acagagcacg caaggtttca gtaacatcac atgctggaac cacagcgccg tcagttccag
     2461 tgccagagca gtaaacgcca tcaacaacca cgcgccccgg ccttgtgaac attggttcaa
     2521 aaatcgcaac ataatcattt ccctgcaata tgcatagagt gcgcagttta aaccaattca
     2581 ttcgctgcgc caccaggagg cagcaaaaat aggacgcaat tggacaatta tcgggcgaaa
     2641 aggcaaaccg ggcatttata tgcccggtaa gttgttatca aagcgttgct atccagccca
     2701 tttgcataaa ccattcggtt acaggggcaa gcgtaaactc gacgcagagc aagccgacga
     2761 gtgtcagtac gagggtgtaa ggcagggcca tccacaccat gcggccataa gagaggcgaa
     2821 tcaatggcgc gagtgcagag gtcagcagga acaggaacgc agcctgaccg ttcggcgtag
     2881 cgacggaggg cagattggta ccggtattaa tggcgaccgc cagcagctcg tattgcttca
     2941 acgtgatagc gccactttcc attgccgctt tcgcttcgtt gatataaatc gtccccacga
     3001 agacgttatc cgaaatggat gacagcagac cgttgaaaat atagaacagc gacagctgag
     3061 catgttccga tgcctgcaac acaaactgaa taattggcga aaacagttgt tggtcgataa
     3121 tcaccgcgac taccgagaaa aacaccgtca acagtgcggt gaatggcaga gattcggtga
     3181 aggctttacc gatagcatgt tcatcggtga caccggtcaa tgatgtcgcc agaataatga
     3241 ctgacaaacc aatcaagcca acttccgcca gatgcaacgc cagcgcagtc accagccaga
     3301 cgccaataat cgcctggaca atcagacgga ttttatcctg acgagtacgc tggtggcggc
     3361 tttgatcgtc aaactgttgc aacacttcgc ggactttctc cggcagcgtt tcaccgtagc
     3421 caaaccaacg cagcttctct accagcaggc aggttaacag gccacaaatc agaaccggaa
     3481 cggtcaccgg cgacatgcgc aggaagaaat cgccaaaatg ccagccagcc gctttagcga
     3541 tgatcaggtt ctgtggttcg cccaccatgg tcatcacgcc gcctaatgca gtgccgacac
     3601 cggcatgcat catcaggcta cgcagaaagc cacggaactg ttccagaacc actttgtaat
     3661 gcttgtcgat atgactatcg tcttgcaggt cggtatcttc agtacgggaa gaggctacgc
     3721 ggtgataaat accataaaaa ccgactgcaa cgctgatcac caccgccacg acggttaagg
     3781 catcgaggaa cgcggagagg aacgccgccg ccacgcaaaa agagagcgac agcagcattt
     3841 tggagcgaat gcttaacagc aaacgggtaa atatgaacag caacagctgt ttcataaaat
     3901 agatacccgc caccataaac atcaataaca gcaagacttc aagatttgcc gccacctctt
     3961 cacggacgtg ttccgcgctg gtcatgccga tgaataccgc ttcgatagcc aacagaccac
     4021 cggggagcag cgggtagcat ttcagggcca tcgccagagt gaaaataaat tccgctacca
     4081 gcaaccagcc cgcgacgaaa gggctgatga ggaaaattaa cgggtttacg attaagaaaa
     4141 tgatgagggc gagtttgtac cagtcggggg actggcccaa aaaattgcgc catagcgcgc
     4201 ggccccagga gatctccatg atggtttccc ttaccttaca aataatcaat gatgttttta
     4261 tgtttaaacg caaagcttaa cggtcaggca ggagtgaggc aagtcttgat agtcaagggg
     4321 aaagagatgc ggaaaatgaa gccttgatcc cttttttctt ctttttgtct gctatcagcg
     4381 tagttagccc tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact
     4441 atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc
     4501 tggaataacc gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta
     4561 attggcgtaa cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg
     4621 ttgaccattc aacatggcaa gccgacgaag gtgaataatt tctgggaaac ctctggttta
     4681 aatatccttg aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat
     4741 ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat
     4801 cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc
     4861 tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt
     4921 tacggtctga tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc
     4981 gccaatccgg aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc
     5041 agtgaaggcg cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc
     5101 gagatttggc accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa
     5161 tcccttccgt ttaaagagca aacccctcaa acgaggggtt ttttgttgtt tttacagatt
     5221 tcccattctt ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc
     5281 gagcatcaca tcaaatcccc acaggcgatg cacatgcttc agcacttctt tgcgcccccg
     5341 atccagcggt gcgcgattat gtggaatgta acgcagcgtc agcgaacggt cgccgcgcaa
     5401 atccacgttc cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa
     5461 ccggttacgg atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt
     5521 atgccgatca tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag
     5581 aaactggctg ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag
     5641 ccagtcggaa ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca
     5701 aatccgttta atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta
     5761 ccacgggctg ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag
     5821 cataaaacgt tccgttactt tcccttcatc atacagatgg ttaaggatgg tatagtgcca
     5881 gaaggtcgcc cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct
     5941 caccttacgc acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt
     6001 ctccataaaa tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg cttcagcaac
     6061 cgttttctct tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgtag
     6121 atactcttcg cgacttttct gccgggcttt ctcttcttgc agtgagattt tttgcgggcg
     6181 tttgtaccgg tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac
     6241 ttcatcaacg ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata
     6301 atcgacaatc gaactggcgt cggtccaact gcggaataag taattgtttt taaagaaaga
     6361 gttatgccca tagcaggcgt gtgccatcac cagcgcctgc atggtaatgg tgttctcttc
     6421 catcaggtaa gcgatacacg ggttagagtt aatgacgatt tcataggcca gtccttgctg
     6481 accgtgctta tacagccgtt cagtttcgat aaacttttta ccgaatgacc agtgcggata
     6541 gttaattggc atgccgacgc tggagtaggc atccatcatc tgttctgagg ttatcacttc
     6601 aatctggtgc gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag
     6661 ataaacatcc agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt
     6721 attcatagaa tcgatcgtcg ccatacgcgc acctcattgt tgtcggcgct ctctgtgtgg
     6781 agcacctcat ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc
     6841 tttgcgattt cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg
     6901 ataaaaaggg cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga
     6961 tgtgagccag ctcaccataa aaaagccgta tgttgaataa tattttcaac tgagttatca
     7021 agatgtaatt agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag
     7081 ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag
     7141 gacatgaggt caccgtcatt gatcgggagc cgggggccgc cctggaaacc agcgccgcga
     7201 atgccgggca aatctccccc ggatatgctg caccgtgggc ggcaccaggt gtgcctttaa
     7261 aagcgatcaa atggatgttc cagcgccatg cgccgctggc ggtccgtctc gacggtacgc
     7321 agtttcagtt gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg
     7381 aaaacaaagg gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc
     7441 gcgccgaaac caatattcag tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta
     7501 ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac
     7561 cgtatcagct gctggaatcc agccgcctgg cggaagtgga gcccgcgctg gcagaagtag
     7621 cgcacaaact gacgggtggt ctgcaactgc ccaatgatga aaccggagac tgccagctgt
     7681 ttacccagaa tctggcgcgg atggcagagc aggcgggggt taaattccgc tttaatacgc
     7741 ccgttgacca actgctttgc gacggcgagc aaatctacgg cgtgaagtgt ggcgatgaag
     7801 tgattaaggc cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag
     7861 gcattgttga tattccggtg catccgctga aaggctactc gctgactatt ccaattgcgc
     7921 aggaagatgg tgcgccagta tccactattc ttgatgaaac ctacaaaatc gccattaccc
     7981 gtttcgataa ccgcattcgt gttggcggaa tggcggaaat tgttggtttt aacaccgagc
     8041 tgttgcaacc gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat ccacgcggcg
     8101 gtcatgtcga gcaggcgact ttctggacgg gtttgcgccc gatgacgcca gacggtacgc
     8161 cggttgtcgg gcgtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg
     8221 gctggacgat ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc
     8281 cagcgatccc atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac
     8341 gtccgggcca tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg
     8401 ccagcctcga tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgccgc
     8461 acgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt gagcgtatct
     8521 ggagcgcgct cggggccacc gatggctttg cattacttaa cctggaagag gcaataacgt
     8581 tacgtgagcg cggctggaag gggccgatcc tgatgctgga aggatttttc catgctcagg
     8641 atctggagat ttatgaccag caccgcctga ccacctgcgt acacagcaac tggcagctca
     8701 aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa gtgaacagtg
     8761 ggatgaatcg gttgggcttc cagcccgatc gcgtgcttac cgtctggcag cagttgcggg
     8821 caatggcgaa tgttggcgag atgaccctga tgtcgcattt tgccgaagcg gaacatcctg
     8881 atggaatttc cagcgcgatg gcgcgtattg agcaggcggc ggaagggctg gagtgtcggc
     8941 gttcgttgtc caattcggcg gcgactctgt ggcacccgga agcgcatttt gactgggttc
     9001 ggcctggcat tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat atcgccaata
     9061 ccggattacg tccggtgatg acgctaagca gtgagattat tggtgtccag acgctaaaag
     9121 cgggtgagcg tgtgggctac ggcggtcgct atactgcgcg cgatgaacag cgaatcggca
     9181 ttgtcgccgc agggtacgcc gacggttatc cgcgccacgc gcctaccggt acccctgttt
     9241 tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg ctagcggtcg
     9301 atttaacgcc ttgcccgcag gcggggattg gtacgccggt tgagctgtgg ggcaaggaga
     9361 tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg atgtgcgcgc
     9421 tggcgttacg cgtcccggtt gtgacagtgt aacttgttgt aagccggatc ggaggcagcg
     9481 tcttctggtt gcgaaaaaat catccatccg gctggtcagc aactgtagtt gttaatgtga
     9541 cagagccatt gcccatgata gtgtccatta aaagaatgga cactatttcc ccggaacctg
     9601 aactcaccgc acaggcgttc tacataaaac gcttacgctt cattgttgac tcgaactcga
     9661 cttcagataa atcacgcttt acccttgatg gagcctgtac atagatttgt gtaattgcct
     9721 gattttgata tgttcaattc aacatcaaat gaaggttaaa ttatggacga caaacaattg
     9781 caggctcagg ctgcgttcag caaagcatcg caaccggcga tagatgcttc attaaattta
     9841 agattcagct tcctcttcag ccacccgtac gccaatcttc aacacttcat tatcttcttt
     9901 ctcggccacc gtccagatca tcccggcaaa ctctacctgg tcaccgacaa ccggtgccgc
     9961 gcctaacaac tgctggacaa tttcacccag cgtttgctgc ttatcacgat attctcgccc
    10021 gtcttccagg ccatatatca gcgcaacatc agcatatttg gcgctggctt cgagaatgaa
    10081 gtcaccaaag aagcgttgat ccagcgcgac cggcggcgac tggctgaaca gtttaccgag
    10141 cgccggaaga tcgcgttccc gaccaattac acacaacaca tcgccttcgc gcagtcgggt
    10201 gctgccggta ggatgaagca actggttatc acgaaacagt gccgcaatac gcgtctcttt
    10261 tggcatatgc aaatcgcgca gtgccgcgcc cacgcaccat ttatcggcac tcaattgata
    10321 aacaaactgc tcccacggat tttccggatg aatatccagg ccaacgcgtg acaccggacg
    10381 tcccactggc ggaaccacca ctttggcttt tttagccgcc cacgagagtg atgttccctg
    10441 caagagcagt gaaaccagaa ccacaaagaa ggcgacatta aagaacagac gtgcattctc
    10501 cagccccgcc atcatcggga acacagccag gatgattggc accgcgccgc gtaatcctac
    10561 ccagctgata aacacgcgct cacgcagatt gaaaccgcgg aaggggagca atccagcaaa
    10621 taccgaaagg ggacgggcga agaatatcat ccatgccgac aaaatgagcg ccggaatggc
    10681 aataggcagc agatcgcttg ggttaaccaa cagccccagc accaggaaca tggcgatttg
    10741 cgccagccag gcgaggccgt cgaaattttg caggatgccg tagcggttgc gaatcggccg
    10801 attacccagc agaaaaccgc acagataaac agccagaata ccgctgcctt ccagcgccgt
    10861 ggttaaagca aaaattaaaa taccgccgct taatgccagc aatggatata atccggcggg
    10921 cagggcgatg cgattaatca tttgcagcag taaataacca ccaccaagtc cgatgacaat
    10981 cccgaggcca aattgctgca gaatatcgac cacgaacatc caactgacac tgctttcatg
    11041 ttgctggatc atcgcaatca gggtaatcgt cagaaagacc gccattggat cattactgcc
    11101 ggattcaatt tccagcgtcg agccaacacg ttcgttaagc cccttaccac ccagcagaga
    11161 aaagaccgct gcagcatcgg tagagccgac gatagcgccg attaataagc cttcaatcaa
    11221 atcaagatta aacagccacg ccgccatcat gccggttaaa ccagaggtga taagcacgcc
    11281 cagcgtcgcc agcgacagtg ccggtcctaa cgccacacga aaggaactgg cctgagtgcg
    11341 catcccgccg tcgagcaaaa taatcgccag tgccaggtta cttaccatgt aggcgaaggg
    11401 gtaattatca aacgggatac cgccgacgcc atcgactcct gccagcatgc cgatcgccag
    11461 aaaaataacc agaataggaa tgccaagacg ggaagaaaat gaactaagta atatactgct
    11521 ggtgacaagg atggatccta agatgaaaag gctaattatt gttgtggcat ccaacggtcg
    11581 gttactcctg attacgctgt ctcttatata aaccctacca tattagcggc agagacagcg
    11641 ttttacttag ccctgaagcg tgattttttt acattttaag aacaggatga ccgctaatcg
    11701 tcaactgagt gccttcccgg gtgttattca gaatggcatg tgcgcccaac ggcagcgtaa
    11761 ccgtgcgttg ttcatgacca aaatcgaggc cggtaatgag tggaatcgac aggcgggaac
    11821 gcaaaaacgc gtacactgac tccaggttgt aacctgcgtc ataatcattg ggcgtgctgc
    11881 cgctaaagct accgagaata atcgccttct gacgtggcaa aattcccgca tgatagagct
    11941 gcaacagcat acgttcgacg cggaaagggt gctcgttaat atcttccagc accagaatac
    12001 cgttctcaat ttttggcatc cacggtgtac caatcagtga aatcagcatc gcaagattgc
    12061 ctccccacaa cgtgccttcg gtctgacatg tcgggccttc cccttgccat tcaatggtga
    12121 aggtttcatt gcgtaacgcc agccagaaat ggtgctcggt aaaggcgttc agttcatccg
    12181 cgccaaagtt tgctaccagc atggggccgc taaaggtgat gacattgcct tgcgccagaa
    12241 gaccgcactg aatggcggta aaatcgctat gcccgcatat gagcaacggg tcatattgtt
    12301 ggcgagcaac cagtgcctgc cagtcaatat ctgccagtaa acgactggca ccgtaaccgc
    12361 cgcgtacagc cagcacgatg gtgttggggg tggtcagtct ggcaagggaa ttgagatctt
    12421 ccaggcgctc cgtttccgta cccgcaaaac gctcacaacg acgggcaata acctcgacgt
    12481 tattgacctg atgcccctcg tcggtaaggc gctggatacc acgcagcgcg gcgtgctgtt
    12541 taatgcagta acccgatggg gcaattaagt gaaacagaga catggcaatt ccttgctgac
    12601 aacagaaacg aaatgtatat catgccgctt aggtgtgccg ttgtcacctc aacggcgatt
    12661 ccaggctata aggatagaag aagtgaaatt gagatggttt gcctttttga ttgtgttatt
    12721 agcgggttgt tcatcaaagc atgactatac gaacccgccg tggaacgcga aagttccggt
    12781 gcaacgtgcg atgcagtgga tgccaataag ccagaaagcc ggtgcagcct ggggcgtcga
    12841 tccacaattg atcacggcga ttatcgctat cgaatcgggt ggtaatccga atgcggtgag
    12901 taaatcgaat gccattggtt tgatgcagtt aaaagcttca acctccggac gtgatgttta
    12961 tcgccgtatg ggctggagtg gtgagccgac gaccagcgag ctgaaaaatc cagagcgtaa
    13021 tatttcaatg ggggcggctt acctgaatat tctggaaacc ggcccgctgg caggcattga
    13081 agatccgaag gtactgcaat atgcgctggt ggtgtcatac gctaacgggg caggtgcgct
    13141 gctacggact ttctcgtcag atcggaaaaa ggcgatcagc aaaatcaacg atttggatgc
    13201 tgacgagttc ctcgatcacg tagcgcgaaa tcatcctgcg ccgcaggctc cgcgctatat
    13261 ctacaaactt gagcaggcac tggacgcgat gtaaatcagt cgcgcacttt gtccgctttt
    13321 tcccgggctt ctcgctcgag agagaaaata atccgctgta attgccgctc caccgtcggg
    13381 ctgacgttaa gaaaacggaa gctcagacgg ggagtggtga tggtttcatt cttgccatca
    13441 atcactttgc gctcgctgat ggagattaac tgggcgtcaa agtgaaaaac accccattgc
    13501 cccatgttga cttcaatctg agcgaagcgc atgccttctt gtaattcggc aggctttgct
    13561 gtttccagta atgcgcccat gccgcctaac gacaaatcat acaggcggaa acgtaacgta
    13621 ctgttatccg ccagtttggt ctggcaaaaa taaggcggat ggagtggggc ggagatgcgg
    13681 aaatatcggc gtcgttgtac aaaccataag gtgggaggcg gtacggtgat aaatgccgga
    13741 agctgcaagt attcactctg ctgtagttgt tcaacagtaa actcgacttt cgcaccctga
    13801 gtttcggcgg taatggtaat gtgctgtgcc tttagcacgg cgatgttgtc ttcggcttga
    13861 ctgccgaaat ccagcaccag tttatccggg gttattgcca gtattttgct gatcagctgc
    13921 ccgccattcc aactgagacg caaaggaatt gcggctttgt gcaaatcgcg taacacgccc
    13981 aggacggcta acggattttg tttcaggaac tgctcatggt aatgactcac gcggaaaaac
    14041 tcctgactga caaactgtct ttggtttatc ggtcacagtt aacaaaactt aatacaaatg
    14101 cgtgaatatt ttttacatgt tgttcttaaa tcagccgaca tacgcccagc atcgatcccg
    14161 cccttgccta tacttagagc gttgacgtaa gcatttcttg cgtccgattc atcgaaacga
    14221 gggcctgaac atgggaatta ttgcctggat tatttttggc ctgatagccg gcattatcgc
    14281 caagctaatc atgccggggc gtgatggtgg tggatttttc ctgacctgta ttctcgggat
    14341 agtcggtgcg gtggtcggcg gctggctggc gaccatgttt ggcattggcg gctccatcag
    14401 tggttttaat ctgcacagct tcctggtggc ggtggtggga gctattctcg ttctgggcgt
    14461 attccgcctc ctgcgaagag aataagattt tcataaggcg gatagcgata cagatgccgc
    14521 tatccgcttt cacatcagaa cgtatactcg acacctgctt tacgggtgga acaaatcaat
    14581 atagcactta gatgcccgat aaagtcggtt cccggtaaca acaacagaat ccttttttaa
    14641 ttattgtttc gttgtttttg gtgtgattca gaaaataaat attttagaca gtagccagtc
    14701 aggcgttaaa aatggggcgg atagtgaggc ggttttggct gcgcttattg aacatttaga
    14761 attgatcaat ccttctggca gattaagcca aaatacccgt tcggcaatgc ttcagttacg
    14821 cgaagaggaa tggtctgaat tctttttttg gttgctaaat tctctggagt gtctggatta
    14881 tgtgattatt aatttagctc cagaaagcaa aaaaacgttg atgagtgaac accgtaacaa
    14941 tattcaggta gcaatcgatg ctttatatag gcaacgtcgg tgtaaatcac cgggtgatga
    15001 aagtgaaaca cttacccgta gaaatgacgc tatcttcggc aaccacgtat ggcaaacatt
    15061 tgcgcaatac ttcccgccag gacttgaaaa accgtctgtg tagaaaacac catagctatg
    15121 gtgttatctg ccgacaacgc catccggaga agccgaatga cgttgtcgga gtaagcgctg
    15181 gttaaggtgt gggttgtgcc tctttggttg agggttgcgt cgttgctgac ttaacggtcg
    15241 gacgcgtcgc cggaacattg tcacacggtt gctctttcgg gcagatcaaa tccagcattt
    15301 tcagcgtcac gccattggtc cagccaaagc catcctgtaa tggatattcg ccaccgccgc
    15361 cccccgttcc ggtggtgctg acatcatatt tttccaccag ctttttctcc cggtcatagg
    15421 tgtgctgaac attggtcagg aagtgccagc taatgtccat cgccacctct ttttgcccgt
    15481 agttttgtaa tccttctgtc gcgacccact gtaacggtgc ccagccattt ggcgcatccc
    15541 attgttgccc acttttcacc gacgtggtgt tcaggccgcc gggttgcagc agatgtgttt
    15601 tcgtcgccgt cgccattttg ttggcgcgat ctttcgctgc cgcattgacg tacagcggga
    15661 acagggcggc cgcggttaac tgattgcgca ctttatgact tttcaggtcg taatcggcat
    15721 accagccttg ttgatcgttc cacatgtatt tttcgatccc tttttgacgg gcatttgcca
    15781 gcgtttcgta ctggtttgcc atcgcgttat ctccggcagc tttgctggcg cgggcgagga
    15841 ttttttccat tttaaacatc aggctgttca gatcgaccgg tacgatgctg gtggtgcgta
    15901 aggtatttag ttgatgcggg ttgtccatcc agcgcgagct gaaatcccag ccagacgcag
    15961 cggcagagcg caggtcgcgg taaatttcag tggcaggtcg attcggattg cttttggcgg
    16021 tggcaatatc ttccacccat gactctggtc gtggcgtatc gcgatcgtcc cagtagcggt
    16081 tgagaagggt accatcctga agttttacaa cgcgtttttc ctgttgtccg gcttgcaggt
    16141 tttcaacacc gtccatccag taagcatatt ctttttgcat ttgcggcagg tattgcttca
    16201 acgcggcatc gccttcatgc tgcgccagta actctaccat cagggcaaag aagggcggtt
    16261 gcgagcggct taaatagtaa ctgcggttgc cgttgggaat atgaccgtaa gtgtctattt
    16321 catgagcaaa attggccacc atatccgcga ctttatccca gtgaccgctt tcggcaagtc
    16381 ctaacatggt gaagtaactg tcccagtaat atacctcgcg aaaacgtccg cccggcacga
    16441 cataaggttc cggcagcggt aacagagaat cccatttttc ggtgttttcg gtagaacgcg
    16501 ttaataccgg ccaaagtccg tcaatatgtt cgcgcagtga ctgcccctct ggcggaacat
    16561 atttctcgcc ctctttcggc agggtgaaat tgacgttaac gaaatggcgc agatcaaatc
    16621 cgctctggtt ttgctgcatc cgataatcag caaggatcat cagcggatcg ctgttcggca
    16681 cggcatcggc aaaggttttt tggtccggaa aaagtttggc gttttgcaca tcattaaaca
    16741 gcggccctaa taaaatatca ggcggctgtg gtgttaccgg tgtttcttct gcctgcaccg
    16801 atagcgcagc gaaacacaaa aagatacagg ctggaattaa cgccattttt tgcgggcgag
    16861 aaggtgcggg ggatttcatc aatcattctc ctttggcgaa accgaataaa acggtattca
    16921 agtatcagaa aaccttagtt caggatcgca ctagctgcat gatcgaaatc tcattctgcg
    16981 aactgtcagg cattttagaa aagggaatta aagggataaa taacggtgaa atagagtgat
    17041 caaataacgt ctgtttatta gccagtcagc gacgttgggt tcgcgattag cgaagaaaaa
    17101 tcatcataaa ttggtaaaaa atataacagg tagttaaaaa acatcagtgc tgggtaaatt
    17161 gccggatgac atcagaacga tgccatccga acggtggctt aaccctgacg gttgaaacgt
    17221 tgcgttttaa cgtccagcgt tagcgtttct tctggttgta tcgcatacag tttctcaccc
    17281 tgctggcaaa tccagccaat ccccagttca cgggcgatta gggcgctgtg ggataccgga
    17341 cttccggcgc taaggcagat accttttaca accgccggat ccagttgcag tactgtggaa
    17401 ggataaatgt tctccgccag tagaatagtt ggcgagttaa actgcgggag ttcttctttc
    17461 gtttgggtca ggtggaccag ggtgcgatgc agaagatcgt ccacatcaat atagcgagct
    17521 tgtagatatt catcatccag ttgctggtat tgctggctaa gttctttaag aacttgctgc
    17581 caggcatatt ctgccgtgca atgttcatgc tgaaggagtt cgcttgccgc cgccagcagt
    17641 tccggatcat ctaacagtgt atggtgacca gaaaagattg cggcaatatc gtcaagcccg
    17701 ctggcttctg ctttcgctgt taacgtcatc agatctaata acgtgaagtc aatagcctgg
    17761 cgtaatcgat cttgttcttc ttccacggtc agggttgatt ttgcctgtac cgtacataaa
    17821 actggttgat aataaaaggc tttacccgaa acaggcggaa cgggacgcag agtaggtgga
    17881 gcgacttcct ccgtttcacc aaagttatct tcagccagct gacggaaagc gatcagtgcc
    17941 tcttcagctt ctggcccttt cgcaatcagg cgcagcgtat cgttatagcg aacttgtagt
    18001 aacgcaatct ggttaatact ctctggtgtg acgcatttgc cgtttttttc cagcaacata
    18061 tcggcattaa atgtcgataa ggtataaacc agccgggagg ccggacgtac atgcaggccg
    18121 ttacggtttt ttatgaccac cgccagagaa cgggcttctt catcgtacgc aggacatgtg
    18181 tcagagattt cagtgtcgga ggacggtaaa cccagttgtt cacgtttggc ttccagcgca
    18241 tgcatagcgt caaagataac tttgtcgata tccgctcccg aggccgcgct gaccgttgct
    18301 gccagtgtac cttcgaccaa cggcgcagca cacaaacgta cttttgcggc gatcttggga
    18361 gccagcaatt ccagcgcagt ttcagcactc aataatgcgc tacccatatc catcatgacc
    18421 agcacatggt cggcatcagc aacagattcg atggcctcca tcactttgac ggcatcggta
    18481 ccaataggat tttgtggatc gtcaattccc gcggcaatgg cgattttaca actatcactc
    18541 attaacatct gacgggctaa ttcaccgaca ccttctccca gtcggctgct atgtgaaact
    18601 atgaccaggt ttaccatcac caattcctta ctcttttgcg gctaacgcca acatttgcat
    18661 cataaacatc accgaggtcg cgccgggatc ctggtgacca atactgcgtt caccgagata
    18721 actggcgcgg cctttgcggg cttgcatcgt aatcgtactt tgtgcagcgg attcggcgat
    18781 tctactggca gcttcgagcg ccaccggaac agagagattt tgctcgctgg actgacgtaa
    18841 cgattccacc accggcaccc acacatcaca catggtttta tcgccaggtt cggctttccc
    18901 gcgactgatt acgccgtccg cgccatcgcg gaacatctga taaagctctt ccagtgtcag
    18961 gctttgccgt gcctgggtcg cctgtgcggc gcggataaag aaggtaccga acagcggacc
    19021 actggcacca ccgacgctgg aaagcagcgt cataccggta ttcttgagaa tgaaaccgat
    19081 atctttatct gcgatagcag ggagtttttc caccactttg ctaaagcctc ggttcatatt
    19141 tagcccgtgg tcagcgtcgc caatttcgcg atccagtccg gtaagatact cgctctcggt
    19201 gctgaaaata tcgccacaac gagtgagcca gttaacaatt tgagttctgc tcagtgacat
    19261 tgctttctcc ttatttaccc cagttaaggg ccggggtgtg gaccggggcg tcccagagtg
    19321 ccagcgtttc gtcatcaact ttcagtaagg tgattgagaa accggtcata tccagtgagg
    19381 tgcagtacgc gccaattaaa ttacgttcga tagtcaatcc cgcttgctgg caacgtgtgg
    19441 tcaggcggtt atagacgccg tacagctcag aaagcggagt tgcgccaaga ttgttaacca
    19501 gcgcaatcac ccgatcgcca gactggagcg gttgtttggt ttgttgttct tcctgccaac
    19561 tgccttgttg ataatcccag aaacgcaaag tgcgatggta tgagccattt tccagcaggg
    19621 tgtcgaacat ttcatcgacg gtttgatcaa gggaagagaa ggagcggcgg tcaatacccg
    19681 gctcaccatg aatgccgacg ccaaactcca tctcattatc cgccagggta aaagaaggtt
    19741 tgcccgcggc aggaacggta caggcaccga gagcgatacc tattgagtgg ccttgattat
    19801 tcaacttacg ccccagttcc gcacaggcgt ccagtgagtc gccacgctcc gccgctgcgc
    19861 ctacgagttt ttcaattaat acggtgttgg caacgccgcg tcgcccggcg gtataaagac
    19921 tgtcttttac cgcaacgtcg tcatcaatga ccacagtggt cacttttacg ccgctatcgt
    19981 gcagtaactc ggtcgctgtt tcaaagttaa gaatatcgcc ggtgtaattt ttgataatca
    20041 acagtacacc ttcgccgcca tcaatttgca tggagcattc aaagatttta tcgggcgtcg
    20101 gtgaggtgaa aatttcgccc ggacaggccc ccgaaagcat cccctgaccg atataaccac
    20161 agtgcatcgg ctcgtgtccg ctgccgccac ccgacagcag ggcgactttt cctgcaacag
    20221 gggcatcagc tcgggtgaca tacaccggat cctgatgcag tgtcagcgat ggatgcgctt
    20281 tcgccagtcc tgccagttgt tcgtccagta cgtcttgcac atcattgatc aattttttca
    20341 ttattttgct ccagcaatta cggtagggca tggatgatgt tcaacgacac ggcgaccact
    20401 gactgccgat gaatccattg tgcatcaggc aagggaaaag aaaattagcc caaattatgt
    20461 ttcatagtga aacatatgct ttaatgaatg ttccatattg aaacttttac gtgtattaat
    20521 acttaaaatt gcgagccgga acaccttttg tcataaggga tgcgggatat gagtggcgct
    20581 tttaacaacg atggtcgggg catatctccc ttaattgcaa cctcctggga gcgatgcaat
    20641 aagctgatga aacgggagac atggaacgta ccacatcagg cccagggcgt gacatttgct
    20701 tctatttatc ggcgtaagaa agcgatgctg acgctcgggc aggctgcgct ggaagatgcc
    20761 tgggaatata tggcaccgcg agagtgtgcg ctgtttatcc tcgatgaaac cgcctgcatt
    20821 ctcagccgta atggcgatcc gcaaaccttg cagcagctaa gtgcactggg attcaatgac
    20881 ggcacgtatt gcgccgaggg aattattggt acttgtgcgc tatcgttagc ggctatctct
    20941 ggtcaggccg tgaaaacgat ggccgatcaa catttcaaac aggcactctg gaactgggcc
    21001 ttttgtgcaa cgccgttgtt tgacagcaag ggccgattga cgggaacaat agcgctggcg
    21061 tgtccggttg agcaaactac cgcagctgat ttgccgttga cgttggcaat cgcccgcgag
    21121 gtcggaaatt tactgctgac ggacagtttg ctcgctgaaa ctaaccgtca tttaaatcaa
    21181 cttaatgccc tgttagaaag tatggatgat ggcgtgatta gctgggacga gcagggtaat
    21241 ttgcaattta ttaatgccca ggcggcgcgg gtcttgcgcc ttgacgcgac ggcaagtcag
    21301 ggaagggcaa tcactgaact cttaacgtta cccgccgtat tgcaacaagc aataaaacag
    21361 gcacatccgc tcaaacacgt agaagcaacc tttgaaagtc agcatcagtt tattgatgcg
    21421 gtgataaccc ttaaaccgat aatagaaacg cagggaacca gctttatttt gttgctccat
    21481 cctgtggaac agatgcggca gttgatgacc agtcaattag gaaaagtcag ccataccttc
    21541 gctcatatgc cacaggacga tccgcaaacc cgccgcttga ttcattttgg tcgccaggcg
    21601 gcgcgcagta gctttcctgt cctgctttgt ggagaagagg gcgtgggaaa ggcactgcta
    21661 agtcaggcaa ttcataatga aagcgagcgt gctgcaggtc cttatatcgc cgtcaattgt
    21721 gagttatatg gtgatgcggc gctggcggaa gaatttattg gtggcgatcg cacggacaat
    21781 gaaaatggcc gtctgagtcg gctggaactg gcacacggcg gcacgctgtt tcttgaaaag
    21841 attgaatatc tggcggtgga gttacagtct gctttgcttc aggttatcaa gcagggtgtt
    21901 atcacgcgac tggatgcgcg gcgtttaata ccagttgatg tcaaagtgat tgccacaacg
    21961 accgcggacc tcgcaatgct ggtggaacaa aatcgtttta gtcgccagct gtattacgcg
    22021 ttgcatgcat ttgaaattac catcccgcct ctgcgtatgc ggcgtggcag cattccggcg
    22081 ctggtgaata acaaattacg cagtcttgaa aaacgcttct ctacgcggct gaaaattgat
    22141 gacgatgccc tcgctcgcct ggtttcctgt gcatggcctg gcaacgattt tgaactttac
    22201 agcgtcatcg agaatcttgc tctgagtagt gataatgggc gcattcgcgt cagtgatttg
    22261 ccggaacatc tgtttaccga gcaggcaaca gatgatgtca gcgccacacg cctttccacc
    22321 agtctgtcat ttgcggaagt tgaaaaagag gcaattatta acgcagccca ggtcacaggc
    22381 ggtcgcattc aggaaatgtc ggctttactt gggatcggcc gcactacgct gtggcggaaa
    22441 atgaagcaac atggcattga tgcagggcag tttaagcgcc gggtatgaaa gacagagacg
    22501 atttctgata catcagagtg atctatattt cattccggcg cacgctaaca tttttcagca
    22561 tcgtttaagg gctggtctat cccgcactta aaagctgaag cgatatcctc cgttgacttg
    22621 cttctgatca aagaggttac tctgcgtgta atccgcttcg aggtagaatg tgtgctgttt
    22681 gttatactgt gcactgactc ccacgccgtt attccagcca ttacctttga aactgtactt
    22741 ctcccgggag ttgttcaaca gatagtctgt atcccctgaa aactcgcgga tagctccagt
    22801 cttcacatac atattcagct ggctgttgcc tgcggtgatg tcatatccca gtatcatgct
    22861 ggcacgcccc agcagcgatt cgtagtgatt cagatgtata ttgagaccat tactcgcctt
    22921 catagccatc tcattctggt ggctgtatgt gagctgggtt tgcggctcta tatagaaccc
    22981 ataaccagta ggggtcaggt tgaacctctg cccggcttcc agggagatgc tcaatccatt
    23041 cgcagtgccg ttggcgttaa cgccgttgtt ctgactgtcc agtacgtgaa aactattttt
    23101 ctggcgcgat gcttttacaa cgagatcgct gtaaaaaccg ttgtgtgcca tgtaactggc
    23161 atacattccc atgtagtctg aacgtgcggt accgtcgcct ccgctatagt ccggcgatgc
    23221 atgtgttgag ccaatataca gaccgacata caacggcatt acatcagaga gacgtttatc
    23281 cccaccaaac tggataccgc tgtaacccat gtcaaagccg ctcagtttgc cactggcgaa
    23341 ggagtccagg cttcccccat aactgcgcag ccagatatta ccgtctttac tctgatttcg
    23401 caggtcaccc attcgttgca tcagcgtacg gttttcaaca tagtttaata agtaaccgac
    23461 attgagataa ttgccgccag catctgcggt cgtggtgggt ttaggtgtgg gagtggggtt
    23521 aggcgtagga tcgggttcag gcgtaggatc ggggttgact atgggaggct gagcgggagc
    23581 cggtgttggt tcaggattag gagtcggttc cggaactgtc ccggaagcgt aaagctccca
    23641 gttagtgcca tttttacgca catcgtacag atatcccccc aactcaacct gagaagacgc
    23701 gctgaacgag gccgcaccgt cagtggtttt taccactgtc agaacttcat ttcccgtagt
    23761 ggcctcgctg ccctggttgc ggatagccaa tacgtgatta ccagcactac ttccgctgat
    23821 attcaataaa tcccctttat tattaacgcc attaccctcg ccaacaacat cagcacgcat
    23881 aataaaggta ctgttaccgc tcaggttctc tacgtttaat gtggcaaatg tgccggcagt
    23941 tgacccgtgg ctggcaaaat cgacagttga atggctcagc gccagcgtgt cgagattaga
    24001 attacttgtt acgttccaga cgctgttatt cattgcaacg tccagtttcc cgccattgac
    24061 attatcgctg agggaggaac ctgtccaaac cgaccctggg tgcatatcta gattgattag
    24121 cccacctttg gaaagaacgc taccgttgat aagcatacga cccgaggcgt tgatgcggct
    24181 ggcggcataa ccatcgtcat gttgcgttgc aatggccatc tggtcgggtg tactcatatc
    24241 aatgaccaga tcgctcgtga ggtcgatctg gctgttggtc atggcataaa ttcccctggc
    24301 tcctgccgcg ccggtgatag ccaaactgtc tccggttatt ctaccgccgc tgagcgccca
    24361 caaacccagc gccagactgc cattacgatc aactgtaata tcggtatttt gcatgttgac
    24421 aacagccgtt gccgtctggg ccgaggcacc ataagaaccg ccggaaaaga tgctgtttcg
    24481 ttgcgctgcc gtgccagaaa aattgattgt cgcgtctgaa ccactggtga cgaggccacc
    24541 gccctgcgcg gaagaaatat ggctatctgc accgatagtg gttgtaccac cacgaacttc
    24601 gacgccattg gccgcggctc cagttacatc aacagtgagt gcattcgcgc taacctggcc
    24661 aaagctccag aggccgtgtg cattatcgcc attggtttta atggaactgt ttgttccgag
    24721 atcgacaaca gagtttttct gtacgtttat ccccatggcg ctgtagccct gaacatcgat
    24781 tgtcaggtct gtagccgtaa aacgcgcagc accattggcg ttattgccgt tgagaccacc
    24841 gatataaaca cctgtacttc catcggtcgt gattttactt ccgcttccaa gatcgacact
    24901 ggtgccatag tcattgatgg ttaggcctat accgttcgag ttttcaatgg tgaattgagt
    24961 ggctgttagt gttgagctat gcccaataat gatgccgtca tcgttactct taatggtgct
    25021 gcctgtgcct aagtcagcat gggtatagtc accaattaag ttaatgccga tggcagaggt
    25081 ttgaccaaca acatctactg tgagtcggtt agctgtcagg gagctgtttt tgccctgaat
    25141 aatgattcct tttgcatatc cagaggcgtc gttgacattg acggtgacat cattacccag
    25201 gttgatattg ccaggcgtgt ttcctgctgg ggtcattaca ccatagaggt ttcctgatgg
    25261 atcggcggta tctcctgtaa ttctgtcacc ttcattaata gtaatattgg tgccatattg
    25321 acttcctgta tcagcagcta tcagcagggg ggaaaagata attgcattca aaccaatagc
    25381 gccaaatttt atcagttgaa ttgagggcga acggattttt aattcagcga gacgatctgc
    25441 tttggtattc ccattgtgtt gtttgatgcc cataccactc ctatatagta cccagactat
    25501 gggtctatgg attttgtcat tttgtttaaa tcaatgaata atatcctctt atcatcatga
    25561 tatttgtatg gtgcaatcat gatgagcttc tc
//
LOCUS       DAZGJW010000064        24254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZGJW010000064 DAZGJW010000000
VERSION     DAZGJW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.064"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            213..365
                     /gene="mokC"
                     /locus_tag="ACHKIK_002602"
     CDS             213..365
                     /gene="mokC"
                     /locus_tag="ACHKIK_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO9083756.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(469..1599)
                     /gene="dnaJ"
                     /locus_tag="ACHKIK_002603"
     CDS             complement(469..1599)
                     /gene="dnaJ"
                     /locus_tag="ACHKIK_002603"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO9083757.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1688..3604)
                     /gene="dnaK"
                     /locus_tag="ACHKIK_002604"
     CDS             complement(1688..3604)
                     /gene="dnaK"
                     /locus_tag="ACHKIK_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO9083758.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3981..4385
                     /gene="yaaI"
                     /locus_tag="ACHKIK_002605"
     CDS             3981..4385
                     /gene="yaaI"
                     /locus_tag="ACHKIK_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO9083759.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4411..5124
                     /gene="msyB"
                     /locus_tag="ACHKIK_002606"
     CDS             4411..5124
                     /gene="msyB"
                     /locus_tag="ACHKIK_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO9083760.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELAELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5273..5839
                     /gene="satP"
                     /locus_tag="ACHKIK_002607"
     CDS             5273..5839
                     /gene="satP"
                     /locus_tag="ACHKIK_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO9083761.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5874..6461)
                     /gene="mog"
                     /locus_tag="ACHKIK_002608"
     CDS             complement(5874..6461)
                     /gene="mog"
                     /locus_tag="ACHKIK_002608"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO9083762.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6576..7529)
                     /gene="tal"
                     /locus_tag="ACHKIK_002609"
     CDS             complement(6576..7529)
                     /gene="tal"
                     /locus_tag="ACHKIK_002609"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO9083763.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7808..9238
                     /gene="yaaJ"
                     /locus_tag="ACHKIK_002610"
     CDS             7808..9238
                     /gene="yaaJ"
                     /locus_tag="ACHKIK_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO9083764.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     IQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            9308..10084
                     /gene="yaaA"
                     /locus_tag="ACHKIK_002611"
     CDS             9308..10084
                     /gene="yaaA"
                     /locus_tag="ACHKIK_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO9083765.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10237..10533)
                     /gene="yaaX"
                     /locus_tag="ACHKIK_002612"
     CDS             complement(10237..10533)
                     /gene="yaaX"
                     /locus_tag="ACHKIK_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO9083766.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(10747..12033)
                     /gene="thrC"
                     /locus_tag="ACHKIK_002613"
     CDS             complement(10747..12033)
                     /gene="thrC"
                     /locus_tag="ACHKIK_002613"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO9083767.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12034..12966)
                     /gene="thrB"
                     /locus_tag="ACHKIK_002614"
     CDS             complement(12034..12966)
                     /gene="thrB"
                     /locus_tag="ACHKIK_002614"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO9083768.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAEIF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12968..15430)
                     /gene="thrA"
                     /locus_tag="ACHKIK_002615"
     CDS             complement(12968..15430)
                     /gene="thrA"
                     /locus_tag="ACHKIK_002615"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO9083769.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDRFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15457..15576)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15512..15577)
                     /gene="thrL"
                     /locus_tag="ACHKIK_002616"
     CDS             complement(15512..15577)
                     /gene="thrL"
                     /locus_tag="ACHKIK_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO9083770.1"
                     /translation="MKRISTTIITTITITTGNGAG"
     gene            complement(15791..16477)
                     /gene="yjtD"
                     /locus_tag="ACHKIK_002617"
     CDS             complement(15791..16477)
                     /gene="yjtD"
                     /locus_tag="ACHKIK_002617"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO9083771.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16877..17017)
                     /gene="yjjY"
                     /locus_tag="ACHKIK_002618"
     CDS             complement(16877..17017)
                     /gene="yjjY"
                     /locus_tag="ACHKIK_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO9083772.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17113..17829
                     /gene="arcA"
                     /locus_tag="ACHKIK_002619"
     CDS             17113..17829
                     /gene="arcA"
                     /locus_tag="ACHKIK_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO9083773.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17889..19241)
                     /gene="creD"
                     /locus_tag="ACHKIK_002620"
     CDS             complement(17889..19241)
                     /gene="creD"
                     /locus_tag="ACHKIK_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO9083774.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(19299..20723)
                     /gene="creC"
                     /locus_tag="ACHKIK_002621"
     CDS             complement(19299..20723)
                     /gene="creC"
                     /locus_tag="ACHKIK_002621"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO9083775.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20723..21412)
                     /gene="creB"
                     /locus_tag="ACHKIK_002622"
     CDS             complement(20723..21412)
                     /gene="creB"
                     /locus_tag="ACHKIK_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO9083776.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQAPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTIDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21425..21898)
                     /gene="creA"
                     /locus_tag="ACHKIK_002623"
     CDS             complement(21425..21898)
                     /gene="creA"
                     /locus_tag="ACHKIK_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO9083777.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22109..22978
                     /gene="robA"
                     /locus_tag="ACHKIK_002624"
     CDS             22109..22978
                     /gene="robA"
                     /locus_tag="ACHKIK_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO9083778.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(22975..23622)
                     /gene="gpmB"
                     /locus_tag="ACHKIK_002625"
     CDS             complement(22975..23622)
                     /gene="gpmB"
                     /locus_tag="ACHKIK_002625"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO9083779.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23674..24195
                     /gene="yjjX"
                     /locus_tag="ACHKIK_002626"
     CDS             23674..24195
                     /gene="yjjX"
                     /locus_tag="ACHKIK_002626"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO9083780.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
BASE COUNT         5759 a         6488 c         5969 g         6038 t
ORIGIN      
        1 ggcgtcttca ggattagaat cgataaaaca gaatgggtta aagagagcga tataatagcg
       61 gcgggtactt gaggctatct gtctcaggca ttagctgaac ggcagataga gaaaagcccc
      121 gagtgatatt ttactatcaa cccgaggccc cctatatgct gaacacatgt agagtgcctc
      181 ttactgaccg taaggtcaag gagaagagag caatgaagca gcataaggcg atgattgtcg
      241 ccctgatcgt catctgtatc accgccgtag tggcggcgct ggtaacgaga aaagacctct
      301 gtgaggttca catccgaact ggccagacgg aggttgctgt tttcacggct tacgaatccg
      361 agtaagtgca acggcgggga gtgatccccg ccacttttca ggtgctcgca tatcttcaac
      421 gcaccctaat tttacccaag cctgcccacg ggcaggcttt tggggaggtt agcgggtcag
      481 gtcgtcaaaa aacttcttca caccatcaaa gaagctcttt gagcgcgggc tgttgtgctc
      541 accggttggg ccaccgaagc tttcttgcag ctcttgcagc agctgtttct gcttctcgtt
      601 caggcctacc ggtgtttcga cgacaacgcg gcacagcaaa tcaccctgtg cgccaccgcg
      661 gacagacttg acgcctttac cgcgcatacg gaaaagctta ccggtctggg tttcgccagg
      721 cactttcagt ttgacgcgac catcaagggt cggtacttcg atttcgccac ccagcgccgc
      781 catagcgaag ttgatcggga cttcgcaata gaggttgttg ccttcacgct cgaaaatcgg
      841 gtgctgttta acctgaacct gaacgtacag atcgcctgcc ggtgcgccat gctcgcccgc
      901 ttcaccttcg cccgcaagac ggatgcggtc tccagtgtcc acccctgccg ggattttaac
      961 ggacagcgtt ttgctgcgct caacacgacc atgaccatga catttgttgc acggatcttt
     1021 gatcagcgta ccgcggccct gacagtgtgg acaggtctgc tgtacagcga agaatccctg
     1081 acgcatctgc acctgaccag aaccatgaca ggtcggacag gtctgcggct gtgtacctgg
     1141 ttttgcaccg ctaccgtggc aaacgtcaca ctcttccaga gtcggaatgc ggatctcttt
     1201 ggtcacgcca cgtacagctt cttcgagggt gagctccatg ttatagcgta aatcagcacc
     1261 gcgcgccgca cgttgacgac cacgtccgcc gccaaaaata tcgccgaaaa cgtcaccaaa
     1321 aatatcgctg aagtctgcgc cgccgccaaa accgccgccg cccataccac cttgctcaaa
     1381 cgcagcatga ccatactgat cgtatgccgc acgtttttgc gagtcggtca gaacttcata
     1441 agcttccttg atctctttaa atttcgcctc ggcctctttg tcaccctggt tacggtccgg
     1501 gtggtatttc atggccaggc gtttgtaggc ctttttgatt tcacgctctt ccgctgtttt
     1561 ggaaacgcct aaaatctcgt aataatcttg cttagccatc ttttttaaat tgcccctaga
     1621 tgaatgcacg ggcggagagg aaattcccct tcgcccgtgt cagtataatt acccgtttat
     1681 agggcgatta ttttttgtct ttgacttctt caaattcagc gtcgacaaca tcgtcatctt
     1741 tcgcgttgtt tgcagaagca tcagcaccgg cagtctgctg ctgggcatgt tgctgctggg
     1801 cgatttccat cagtttctgg gaaacctgtg ccagctcctg cattttcgct tcgatagcgg
     1861 ctttgtcttc acctttcaga gcagtttcca gtgcagtcag tgcagactcg atagcagttt
     1921 tgtcgtcagc cggcagtttg tcgcctgctt cttcaacctg cttacgggtg ctgtgcagca
     1981 gatggtcgcc ctggttgcga gtctgtacca gctcttcaaa cttacggtca gcttcggcgt
     2041 tagcttctgc gtcgcgtacc attttctgga tttcatcttc gttcagacca gaagaagcct
     2101 tgatggtgat cttctgctct ttaccgctgt ttttatcttt cgcggaaacg tgcaggatac
     2161 cgtcagcatc gatatcgaag gtaacttcga tctgcggcat gccgcgcggt gccgggttga
     2221 taccatccag gttgaactga cccagagatt tgttatcagc cgcacgttta cgttcaccct
     2281 gcagcacatg gatggttacc gcagactggt tgtcttcagc ggtagagaac acctggctgt
     2341 gcttggtcgg gatagtggtg tttttcgcga tcagcgtcgt catcacaccg cccatggttt
     2401 cgatacccag agacagcggg gtaacgtcca gcagcagtac gtctttcacg tcaccagtca
     2461 gaacaccacc ctgaacagca gcaccgattg ctacagcttc gtccgggtta acgtctttac
     2521 gcggctcttt accaaagaac tcagcaactt tcttctgaac cattggcata cgagtctgac
     2581 caccaacgag gataacgtcg tcgatatcag atacggacag gccagcgtcc tgcagtgcaa
     2641 ctttcagcgg ctcaatggaa cggtttacca gatcttcaac caggctttcc agtttcgcac
     2701 gagtcacttt gatgttcatg tgtttcggac cggtcgcatc tgcagtgatg tacggcaggt
     2761 taacgtcggt ctgctgagcg gaagacagtt cgattttcgc tttttctgcc gcttctttca
     2821 ggcgctgcat tgccagcgga tcgttgcgca ggtcaatgcc ctgatctttc ttgaattctt
     2881 caaccaggta gttgatcaga cggctgtcga agtcttcacc acccaggtgg gtatcaccgt
     2941 tggtagccag aacttcgaag gttttttcgc cgtcaacttc gtcgatttcg ataatagaaa
     3001 tatcgaaagt accacccccc aggtcataaa ccgcgatagt acggttgcca gtacctttgt
     3061 ccagaccgta agccagcgca gctgcggtcg gttcgttgat gatacgtttt acttccagac
     3121 cagcgatacg gcctgcgtct ttggttgcct gacgctgagc atcgttaaag tatgccggta
     3181 cggtgataac agcttcagtt accggttcac ccaagtaatc ttcagcggtt ttcttcattt
     3241 ttttcagcac ttcagcagaa atctgcggcg gtgccatttt ctggccttta acttcgaccc
     3301 atgcgtcgcc gttatcagca gcaataattt tgaacggcat gatggaaaca tcacgctgta
     3361 cttcttcgtc ctggaagcgg cggccaatca ggcgtttaat cgcaaacagg gtgttttgcg
     3421 ggttcgtcac tgcctgacgt ttagccggct gaccaaccag agtttcacca tcctgggtat
     3481 aggcaataat agaaggcgtg gtgcgatcgc cttcggcgtt ctccagcacg cgaggagtgg
     3541 tgccatccat aatcgctaca caagagttgg tagtacccag gtcgatacca attattttac
     3601 ccatctaaac gtctccacta tatattcggt catcatgtgg ttgtgagtct gtaatagggg
     3661 cgaaacgtct ggtttcaact gcccaatttc atttttttgc agactcactc actgcggttg
     3721 actactaaat ggggtcgtaa cccacgtcat caagggggag atgcaaaaat tttttgtgct
     3781 tttgcgcaga aatttcgcga tatagcagga caagacgtga aactgacagc aattttattc
     3841 atataaagaa tgaatcgcag ccaagacatt catcaaaaaa ttgtaataaa aagaaaacat
     3901 tacgcacctg aatcttctct ttatcagcag taaactagtg ggtatccatc cccctacctc
     3961 tccccactaa gagaatcctt atgaaatccg tttttacgat ttccgccagc ctggcgatta
     4021 gtctgatgct gtgctgcacg gcgcaggcaa acgaccataa aatcctcggc gtcattgcga
     4081 tgccacgtaa cgaaaccaac gatctggcgc tgaaacttcc tgtttgccga attgttaaac
     4141 gcatacaact ctctgccgac catggcgatt tacagttaag cggtgcatcg gtttatttca
     4201 aagccgcccg tagcgccagt cagagcctga atattccttc agaaataaaa gaagggcaaa
     4261 ccactgactg gatcaacatt aacagcgata acgacaataa acgctgcgtc tcaaaaatca
     4321 ccttttccgg tcatacggtg aactcatcgg atatggccac gctgaaaatt atcggcgacg
     4381 actaacgcca gaatatagaa gccacaaaaa atgaatgtta attacctgaa tgattcagat
     4441 ctggattttc tccagcattg cagtgaggaa cagttggcaa atttcgcccg attgctcacc
     4501 cataatgaaa aaggcaaaac tcgcctctcc agcgtactga tgcgcaacga actgtttaaa
     4561 tctatggaag ggcatcccga gcaacatcgc cgcaactggc agctgattgc tggagaatta
     4621 cagcattttg gtggcgatag tatcgccaac aaactgcgcg gacacggtaa attgtatcgg
     4681 gccattttgc tcgatgtttc aaaacgattg aagctgaaag ccgacaaaga gatgtctacg
     4741 tttgagattg agcaacagtt actggaacaa tttctgcgta acacctggaa gaatatggac
     4801 gaggaacata aacaggagtt tctgcacgcg gtcgatgcga gggtgaatga gctggcagag
     4861 ctgctgccgc tgctgatgaa agacaaatta ttggcaaaag gcgtgtcgca tttgctttcc
     4921 agccaactga cccgcatttt acgcacccac gcagcaatga gcgtacttgg gcatggtttg
     4981 ctgcgcggcg cggggctggg aggccctgta ggtgcggcac taaatggggt taaagcggtc
     5041 agcggcagcg cctatcgcgt gacgattcca gccgtactgc aaatcgcctg cctgcgccgg
     5101 atggttagcg ccactcaggt ctgataccgg aaaccgggga aaattttcat tttcccctca
     5161 aaagatcgta gacactgccc cactggctga ttattatgcc gcgccctgaa aacactacag
     5221 ttattcaggg aaattatttc accattcatt cgatgatgat ttttgaggaa ttatgggcaa
     5281 cactaagttg gctaatccgg caccgctggg cctgatgggc ttcggcatga ccaccattct
     5341 gcttaacctg cacaacgtgg gttatttcgc tctggacggt attattcttg ccatgggcat
     5401 tttctacggc ggcatcgcgc aaatttttgc cggtctgctg gagtacaaaa aaggcaacac
     5461 tttcggttta accgcattca cctcttacgg ttctttctgg ctgacgttgg ttgcgattct
     5521 gctgatgccg aaactgggtc tgaccgatgc gccaaatgca cagttccttg gtgtctacct
     5581 gggtctgtgg ggcgtattta cgctgtttat gttcttcggc acgctgaaag gcgcacgcgt
     5641 tctgcaattc gttttcttta gcctgactgt gctgtttgcc ctgctggcga tcggtaacat
     5701 tgccggtaac gccgcaatca tccactttgc cggctggatt ggtctgatct gtggtgccag
     5761 tgcaatctat ctggcgatgg gtgaagtact gaacgagcag tttggtcgca ccgttctgcc
     5821 gattggtgaa tcccactaat tgttttgaga tccccccgct cggggggatt tttttattcg
     5881 ctaacgtcgc gtcttgcact cttcggtctg aatgcagcaa ccacttctgg tgccgtttca
     5941 acgtatggcc cttccagcaa ctgaatgcag tacggtacgc tggcaaaaat accgtgtacc
     6001 acaacgttac ccgcagcgtc cttcacacct tccagcgtct ctttaataga cttcggctga
     6061 ccgggtaagt taaggatcag cgcctgtttg cgaatcacac caacctgacg cgaaaggatc
     6121 gcagttggta caaaatgcag gcttatctgg cgcatctgtt caccaaagcc aggcatctcg
     6181 cggtccgcta ctgccagcgt cgcatcgggc gttacgtcac gacgcgccgg gccagttccg
     6241 cccgtggtga gcaccagatg gcaactcatt tcatccacta gctcacacaa cgtttgctcg
     6301 atgatcgcct gctcatcggg gattaagcgg gtttccagtt caaacggcgt ggttagcgcc
     6361 gatgtcagcc attcttccag cgcagggatg cctttatcct gataaacgcc gctggatgcg
     6421 cgatcagaga tggaaactaa gccaatacgt aaagtattca tcaagatttt tccgtttaaa
     6481 cagtctcgtt aaacagaatg atacactgcg aagggagtga cagacaggct tcagaagagg
     6541 tagcgtgacc gacttcccgg tcacgctaag aatgattaca gcagatcgcc gatcattttt
     6601 tccagttttt cctggtcaat agcaaactta cggatacctt ccgccagttt atctactgcc
     6661 attggatcct ggttgtgctg ccacaggaac tcggactcag tgatacgcgc cggacgcgct
     6721 ttcacttcgc cggtgtaaga cagtttacgt tcgatagccc cttcgctctc agccagctct
     6781 ttcagcagtg taggtgcgat ggtaagacgg tcgcagcctg ccagttccag aatttcgccg
     6841 atgttacgga agcttgcgcc cataaccacg gtttcataac cgtgctcttt gtagtactgg
     6901 tagatttcag atacagaaac cacgcccgga tcttctgccg gagcgtactc tttcttatcg
     6961 gtattcgctt tgtaccagtc aagaatacgg ccaacaaacg gcgagatcag gaacacgccc
     7021 gcttccgcac aagcacgagc ctgagcgaag gagaacagca gggtcaggtt acagttgatg
     7081 ccttcttttt ccagctgttc tgcagcacgg ataccctgcc aggtagaagc cagtttgatc
     7141 agaatacgat cgttgctaat accagcatcg ttgtagagtt tgatcaggcg ttttgctttc
     7201 gcaattgacg cttcggtgtc ataggaaaga cgcgcatcaa cttcagttga gatacggccc
     7261 ggaaccagtt tcaggatttc cagaccaata tttactgcca gtttgtcggt cgcgtccacg
     7321 atctgctgcg cgcgatcgtt gctctgctgt ttcgcccagg cgacagcatc atcaatcaac
     7381 ttacggtatt ccggaatctg cgctgcgtta agaatgagag aagggttggt tgtggcatcc
     7441 tgcggttgat acagcttcat tgccgcgatg tccccagtgt cggccactac ggtggtgtac
     7501 tgacgaaggg aggtcaattt gtccgtcatg atagtatttc tctttaaaca gcttgttagg
     7561 gggatgtaac cggtctgccc tgatgatatc acgacgcaac gtcagcgcaa ccgccgacaa
     7621 gacggttatc gccaggcgag actgtttcgg atttctgaca ctgcgttgat atcgcccgcc
     7681 atttttatac aaaacctcat gtatgctacg cagaagttat caagtacctc gtagcgtata
     7741 tacttcttaa acaattggta acgtttacac aggaaagtca tcgcgaccgg caataagagg
     7801 gatatgcatg ccagattttt tctcctttat taacagcgtc ctttggggat cggtaatgat
     7861 ttacctgctc ttcggcgcag gttgttggtt cacttttcgc accggatttg tgcagtttcg
     7921 ctacatccgc cagtttggca aaagtcttaa aaatagcatt catccacagc caggcggttt
     7981 aacctcattt cagtcattgt gtaccagtct tgcggcgcgc gtgggtagcg gcaacctggc
     8041 cggcgttgcg ctggcaatta ccgccggtgg acctggagcc gtcttctgga tgtgggttgc
     8101 cgcgtttatc ggcatggcga cctcgtttgc cgaatgttcc ctcgcacagc tttataaaga
     8161 acgtgatgcc aatgggcagt ttcgtggcgg accggcatgg tatatggcgc gcgggctggg
     8221 gatgcgctgg atgggcgttc tgttcgccgt ctttttgctc atcgcctacg gcataatttt
     8281 cagcggaatt caggcgaacg ccgttgcgcg agccctgagt ttttcttttg attttccccc
     8341 gctggtgaca ggtattattc tcgctgtctt tgctctgctg gcgatcactc gcggtcttca
     8401 tggcgtcgcc cggctcatgc aggggtttgt cccgttgatg gcgataatct gggtactgac
     8461 cagcctggtg atttgcgtaa tgaatatcgg gcaacttccc cacgtcattt ggtctatttt
     8521 tgagagtgct tttggctggc aggaagcggc aggcggcgcg gcgggatata ccttaagcca
     8581 ggcgattact aacggttttc agcgcagtat gttttccaat gaggcgggaa tggggtcgac
     8641 gccaaacgcg gcagcggcag cggcgtcctg gcctccgcat ccggcagcgc aagggattgt
     8701 ccagatgatt ggcattttta tcgacaccct ggtcatctgt acggcaagcg ccatgctgat
     8761 attactggcg ggtaacggca caacctacat gccgctggaa ggtattcagc ttatccagaa
     8821 ggcgatgcgg gtgctaatgg gttcctgggg tgctgagttt gttaccctcg tggttattct
     8881 gtttgccttc agctccatcg ttgccaacta catttacgcc gaaaacaatc tcttcttttt
     8941 acgcctgaac aaccctaaag cgatctggtg tttgcggatc tgcaccttcg caacggtcat
     9001 cggcggcacc ttgctaagtc ttccgctgat gtggcaactg gcagatatca taatggcctg
     9061 catggctatt accaatttga ccgccatttt actgctctcg cctgtggttc ataccattgc
     9121 cagtgattat ctacgccagc gtaaactcgg cgtgcgcccg gtgtttgatc cgttgcgtta
     9181 tccggatatc ggccgccagc tttctccgga cgcgtgggat gatgtttcgc aggagtaatc
     9241 acaactatcg atcaactcat tctctttttt tgctaaagtc ggcataaatt tcctgcaagg
     9301 actggatatg ctgattctta tttcacctgc gaaaacgctt gattaccaaa gcccgttgac
     9361 caccacgcgc tatacgctgc cggagctgtt agacaattcc cagcagttga tccatgaagc
     9421 gcggaaactg acgcctccgc agattagcac gctgatgcgc atcagcgaca aactggcggg
     9481 tatcaacgcc gctcgctttc atgactggca gccagatttc acgccggaga atgcccgcca
     9541 ggcgattctg gcgtttaaag gtgatgtcta caccggcttg caggccgaaa ccttcagcga
     9601 agacgatttc gattttgccc aacagcattt gcgaatgctt tccggcttgt atggcgtact
     9661 gcgcccgctc gatttaatgc agccttatcg tctggaaatg gggatccgtc ttgagaatgc
     9721 ccgagggaaa gatctgtatc aattctgggg agatatcatc accaacaagc tgaacgaggc
     9781 gctcgcagca caaggcgata atgtggtgat taatctggcg tcagatgagt attttaaatc
     9841 ggtgaagccg aagaaattga atgccgagat tatcaagccg gtgttcctcg atgagaagaa
     9901 cggcaaattt aagataatca gcttctacgc taagaaagca cgcggtctga tgagtcgttt
     9961 tattattgaa aatcggctga ccaaaccaga gcaactgact ggttttaata gcgaaggtta
    10021 cttctttgat gaagcttcct ccagcaatgg cgaactggtg tttaaacgct acgagcagcg
    10081 ttaagtttgc agtcaatgcc ggatgcggcg tgaacgcctt atccggccta caaaagcatg
    10141 cagattcaat atattgcaga attcacgtag gcctgataag acgcgccagc gtcgcatcag
    10201 gcgctgaatg ccggattgtt acccggcatt tgtcatttag cggtgatgtt tgcctggacc
    10261 atgaccgccg tgatgatcat gatgagcttt cttatggtga cgcggcggtg gcggcggtcc
    10321 gtgtgggtgc cagcgattgc ctcgccattc ataatgttgt ttccaccagc cgtggtcgcg
    10381 ccagtgaccg ccatcccagt aataaccgcg attatcacga tcgcctatct gtaattttac
    10441 tgacgggact aacgtaattt ccgcagcctg cgctgccatg ggagcgacca gaaccaggga
    10501 aagtgcgagt acgatagatt gcatcttttt cacaggttac tccttttatc attgcccgtt
    10561 atgggtcgat atccggagat tacgcgatga gaacggcggt tgttattctc cgcaatccta
    10621 atctctaagt taccgcattt attgagaatt tctccttttt ccctgtcgtt tctctccata
    10681 ttttcttcac aaaaaagccg ggctgcataa aagcaaaccc ggcctgattg agataatgaa
    10741 tagatgttac tgatgattca tcatcaattt acgcaacgca gcaaaatcgg cgggcagatt
    10801 atgtgaaagc aagggtaaat cagcacgttc tgccagctct tttggcagat ccaacgtttc
    10861 accgagaatc gcttccacgc tctctttaaa tttcgccgga tgcgcggtgc cgaggaacaa
    10921 gccatattcg cctggattca actggtcacg cagcgcacga tacgctacgg cagcgtgcgg
    10981 ctccgaggtg tagcccagtt cttttaactc acgcattgtc tgttgcgtgg tttcatcatc
    11041 cacggctgca taacccagct ctttcagttg ccagattttg cggcggaaca actcttccac
    11101 acgcggccag ttgttcggct gactcacgtc catcgcgttg gataacgtcg cctgagtcgc
    11161 tttgggggac cactgaccgt cgtgcaggaa acgtggcacg gtatcgttca cgttggtcgc
    11221 agcaataaaa cgtttcaccg gcagaccgag tgacttcgcc agcagacccg ccgtcaaatc
    11281 gccgaagttt ccgcttggta ccgaaacaac cagctggttg cgcgcctcct gcggcagctg
    11341 cgcaacagct tcaaagtagt agcaaatctg cgccagcaaa cggctgatgt taatcgagtt
    11401 agctgagttt aaccccagcg ccactttcag ttcttcatca tcaaacgcct gcttcaccag
    11461 cgcctgacag gcatcgaaat cgccgtcgat ggcaacagtt tcgatattgc cgcccaatgt
    11521 acagaacagt ttttcttgca gtggactgat tttgcctcgt ggatagagga taaccacttt
    11581 cacattcggc aaaccgtaga aagcatgagc cactgccgct ccggtatcac cggaggtcgc
    11641 ggtcagaatg gtcactggct tatcacccgc aatatgggtc agcatttgtg ccataaagcg
    11701 accgccgaaa tctttaaatg ccagcgttgg cccgtggaac aattccagac aaccgacatc
    11761 gctttcaaca ttggcgaccg gagccgggaa ggcaaacgcc gcgcgcacgc gctcttccag
    11821 gatttcctgc gggatttcat caccaataaa cgccgagagg atcttcgcac tgcgggtgac
    11881 aaaatccagc ttcagcatct catcaatttc agtcaggctg aattccggca ggtcatgcgg
    11941 aaaaaacagc ccctgatttt tgcccaaccc ctgggttacg gcttgcgcaa agctgacctg
    12001 ctcgttgtga tctttcagat tgtagagttt catttagttt tccagtactc gtgcgcccgc
    12061 cgtatccagc cggcaaatat gaacaaaacc ttcctgattt tgcaggtagt tcttacccaa
    12121 ccagtcggca acgcgctggg cggtatccgg cttgtcacac agagcgaaca aggtcgggcc
    12181 ggagccggag ataccgctcg ctaccgcgcc gatttccgcg accgcctgcc gcgcctgccg
    12241 gaagcctggc agtaatcgtt cacggtaggg ttcagcgata acatctttca tcagcttcgc
    12301 ggcaagctca ggctgacggg aatagcaggc gtgaatgaag cctgccagat gtcgcccgtg
    12361 cgcaatgcaa tcctggcggc gatactgcgc cggtaaaata gcccgggctt ctgccgtcga
    12421 gactttaatc cccggatacg ccagcaccca cagccactca tcaaacccag gcacttgctg
    12481 gctgatgatg tcgttttctt cgatcatcaa ctgcatacca cctagaaaac acggtgccac
    12541 gttgtcgtaa tgaatgctgc cggagatacg tccttccagc tcgcccatca aagccagcaa
    12601 acgagtgtca ttaagcggct tgccgcagtg ttcattcatc gccatcagcg ccgcgaccac
    12661 cgaacaggcg ctggagccta agcccgaacc gatcggcata ttcttttcca gagtcatcgc
    12721 cactggaatt tgcttgccaa gctcctggca aaaacgctcc cagcactgat aaacgatatt
    12781 ttcccgtggt tctgacggca gcttatcggc aaagcgcccg aggttgttga gactgaatat
    12841 ctctgccgcc tcaaccgtaa ctacatctcc gagcaatgca ccatcaacgg gtgtcaccgc
    12901 cgccccgagc acatcaaacc cgacgctcat attggcactg gaagccgggg cataaacttt
    12961 aaccatgtca gactcctaac ttccatgaga gggtacgcag cagatcggca aagacaccag
    13021 cagcggtaac gtcattgccc gcaccatatc cgcgcagcac caacggcagc ggctgataat
    13081 agtggctata gaaggccagg gcgttttcgc catttttcac tttgaacagc ggatcattac
    13141 catccacttc ggcaatcttc acgcgacaga cgccatcttc atcaatattg ccaacatagc
    13201 gcaaaacttt tccttcatca cgggccttcg ccacgcgcgc ggcaaagcga tcgtcgagct
    13261 gtgacagatt cgccataaaa gcggcaacat caccctcggc gttaaactct gcgggcagca
    13321 caggttcaat ttcaatatcc gccagctcca gttcacgtcc cgtttcacgg gcgagaatca
    13381 atagtttacg cgccacatcc ataccagaaa gatcatctcg cggatccggt tcggtataac
    13441 ccatttcccg tgccagcgtg gtcgcctcgg agaaactcat gccttcgtct aacttgccga
    13501 agatataaga aagcgatcct gaaagaatgc cggagaactt catcaattca tcaccagcat
    13561 tgagcagatt ttgcaggttt tcaataaccg gtaatccagc cccaacgttg gtgtcataga
    13621 ggaatttccg ccgcgatttt tccgccgcat gacgcaacag atggtagtaa tccatcgacg
    13681 aggtgttggc ctttttgttc ggcgtgacaa cgtggaaacc ttcgcgcagg aagtcggcat
    13741 attgatccgc cactgcctgg ctggaggtgc agtcaacaat gaccgggttc agcagatgat
    13801 attctttcac gaggcgaatt aagcgcccga gattaaacgg ctctttggct tgcgccagtt
    13861 cttcctgcca gttttccaga tttaggccat gcacattggt gagcagtgcc ttcgagttgg
    13921 caacaccgca gacacgtaag tcgatatgtt tattcttcag ccagctttgt tgacgcttca
    13981 gttgctccag cagcgcaccg ccaacgccac cgacgccaat cacaaacact tcgataacct
    14041 gatcggtatt gaacagcatc tgatgagtaa cgcgcacgcc agtggtcgca tcatcgttat
    14101 ttaccacgac agagattgag cgttcagaag atccctgagc aatagcgaca atgttgatat
    14161 tggcgcgggc cagcgcggca aagaatttcg ccgagatccc acgcaaggta cgcataccat
    14221 cacctaccac cgagataatg gccagccgtt ccgtcaccgc cagcggctcc agtaagcctt
    14281 ctttcagttc caggtagaac tcttcctgca ttgcccgttc agcccgcaca cagtcgcttt
    14341 gcggaacgca gaaactgata ctgtattcgg aagatgattg cgtaatcagc accacggaaa
    14401 tacgggcgcg tgacatcgcg gcaaagacgc gcgccgccat gccgaccatt cctttcatcc
    14461 ccgggccgga aacgctgaac attgccatgt tattcagatt ggaaatgccc ttgaccggta
    14521 attcgtcttc atcacgactg gcaccaatga gcgtacctgg tgcttgagga tttccggtat
    14581 ttttaatcag gcaagggatc tggaactggg cgatgggggt aatggtgcgg gggtgaagaa
    14641 ctttagcgcc gaagtaggaa agctccatcg cttcctggta ggacatcgac ttcaacaacc
    14701 tcgcatcggg cacctgacgc gggtcgcagg tatagacccc gtcaacgtcc gtccaaatct
    14761 cgcaacaatc ggcgcgtaaa caggcagcca gcaccgccgc ggagtagtcg gaaccgttgc
    14821 gtccaagtac caccagttcg cctttttcat taccggcggt gaagcctgcc atcagcacca
    14881 tgtgatcagc cggaatacga cttgccgcaa tacggcgggt ggactcagca atatcgacgg
    14941 tagattcgag gtaatgcccc actgccagta gtttttcgac cggatcgata acggtaacgt
    15001 tgtgaccacg cgcttctaac acgccggcca taatggcgat cgacattttc tcgccacggc
    15061 aaatcagcgc agcgttaatg ctatccgggc actgccctaa caaactaatg ccatgcagga
    15121 catgttttat ttgggcaaat tcctggtcga cgaaagtttt caattgcgcc agcgggaatc
    15181 ccggctgggc ggcggcgagt cccgtcaaaa gttcggcaaa aatacgttcg gcatcgctga
    15241 tattgggtaa agcatcctgg ccgctaatgg ttttttcaat catcgccacc aggtggttgg
    15301 tgattttggc gggggcagag aggacggtgg ccacctgccc ctgcctggca ttgctttcca
    15361 gaatatcggc aacccgcaga aaacgttctg catttgccac tgatgtaccg ccgaacttca
    15421 acactcgcat ggttgttacc tcgttacctt tggtcgaaaa aaaaagcccg cactgtcagg
    15481 tgcgggcttt tttctgtgtt tcctgtacgc gtcagcccgc accgttacct gtggtaatgg
    15541 tgatggtggt gataatggtg gtgctaatgc gtttcatgga tgttgtgtac tctgtaattt
    15601 ttatctgtct gtgcgctatg cctatattgg ttaaagtatt tagtgaccta agtcaataaa
    15661 attttaattt actcacggca ggtaaccagt tcagaagctg ctgtcagaga ctcttttttt
    15721 aatccacaca gagacatatt gcccattgca gtcagaatga aaagctgaaa aatacttact
    15781 aaggcgtttt ttatttggtg atattttttt caatatcatg cagcaaacgg tgcaacattg
    15841 ccgtgtctcg ttgctctaaa agccccaggc gttgctgtaa ccagtcgacc agttttatgt
    15901 catctgccac tgccagagtc gtcagcaatg ccatgactcg ttcgcgtaaa gcttgcagtt
    15961 gatgttggtc tgccgttgtg tcacttttcg tcggttgttg tattaatgtt gctaattgat
    16021 agcaatagac catcactgcc tgccccagat tgagcgaagg ataatccgcc accatcggca
    16081 caccagtaag aacgtcagcc aacgctaact cttcgttagt caacccggaa tcttcgcgac
    16141 caaacaccag cgcggcatgg ctcatccatg aagatttttc ctctaacagc ggcaccagtt
    16201 caactggcgt ggcgtagtaa tgatatttcg cccgactgcg cgcagtggtg gcgacagtga
    16261 aatcgacatc gtgtaacgat tcaaccagtg tcgggaaaac tttaatatta tcaataatat
    16321 caccagatcc atgtgcgacc cagcgagtgg ctggctccag atgtgcctga ctatcgacaa
    16381 tccgcagttc gctaaacccc atcgttttca ttgcccgcgc cgctgcccca atattttctg
    16441 ctctggcggg tgcgaccaga ataatcgtta tacgcatatt gccactcttc ttgaccaaat
    16501 gaccgcgaac agggtgatca ctgtcaactt attacgcggt gcgaatttac aaatccttaa
    16561 cgtaagtcgc agaaaaagcc ctttacttag cttaaaaaag gctaaactat ttcctgactg
    16621 tactaacggt tgaattgtta aaaaatgcta catatccttc tgtttactta ggataatttt
    16681 ataaaaaata aatctcgaca attggattca ccacgtttat tagttgtatg atgcaactag
    16741 ttggattatt aaaataatgt gacgaaagct agcatttaga tacgatgatt tcatcaaact
    16801 gttaacgtgc tacaattgaa cttgatatat gtcaacgaag cgtagtttta ttgggtgtcc
    16861 ggcccctctt agcctgttat gttgctgtta aaatggttag gatgacagcc gtttttgaca
    16921 ctgtcgggtc ctgagggaaa gtacccacga ccaagctaat gatgttgttg acgttgatgg
    16981 aaagtgcatc aagaacgcaa ttacgtactt tagtcatgtt acgccgatca tgttaatttg
    17041 cagcatgcat caggcaggtc agggactttt gtacttcctg tttcgattta gttggcaatt
    17101 taggtagcaa acatgcagac cccgcacatt cttatcgttg aagacgagtt ggtaacacgc
    17161 aacacgttga aaagtatttt cgaagcggaa ggctatgatg ttttcgaagc gacagatggc
    17221 gcggaaatgc atcagatcct ctctgaatat gacatcaacc tggtgatcat ggatatcaat
    17281 ctgccgggta agaacggtct tctgttagcg cgtgaactgc gcgagcaggc gaatgttgcg
    17341 ttgatgttcc tgactggccg tgacaacgaa gtcgataaaa ttctcggcct cgaaatcggt
    17401 gcagatgact acatcaccaa accgttcaac ccgcgtgaac tgacgattcg tgcacgcaac
    17461 ctgctgtccc gtaccatgaa tctgggtact gtcagcgaag aacgtcgtag cgttgaaagc
    17521 tacaagttca atggttggga actggacatc aacagccgtt cgttgatcgg ccctgatggc
    17581 gagcagtaca agctgccgcg cagcgagttc cgcgccatgc ttcacttctg tgaaaaccca
    17641 ggcaaaattc agtcccgtgc tgaactgctg aagaaaatga ccggccgtga gctgaaacca
    17701 cacgaccgta ctgtagacgt gacgatccgc cgtattcgta aacatttcga atctacgccg
    17761 gatacgccgg aaatcatcgc caccatccac ggtgaaggtt atcgcttctg cggtgatctg
    17821 gaagattaat cggcgttacc accgtcaaaa aaaatggcgc tttttagcgc cgtttttatt
    17881 tttcaacctt atttccagat acgtaattga tcgtccattg taacttcttt actggctttc
    17941 attttcggca gtgaaaacgc ataccagtcg atattacgag tcacaaacat catgccggcc
    18001 agcgccacca ccagcacact ggttcccaac aacagcgcgc tatcggcaga gttgagcagt
    18061 ccccacatca caccatccag caacaacagc gcgagggtaa acaacatgct gttgcgccag
    18121 cctttcaata ccgcctgcaa ataaataccg ttcattaacg ccccaatcag actggcgatt
    18181 atccatgcca cggtaaaacc gatatgttca gaaagcgcca gcaagagcaa ataaaacatc
    18241 accaatgaaa gccccaccag caaatattgc attgggtgta aacgttgcgc ggtgagcgtt
    18301 tcaaaaacaa agaacgccat aaaagtcagt gcaatcagca gaatggcgta cttagtcgcc
    18361 cggtccgtta attggtattg atcggctggc gtcgtcactg cgacgctaaa cgcggggaag
    18421 ttttcccagc cggtatcatt gcctgaagca aaacgctcac cgagattatt agcaaaccag
    18481 ctgctttgcc attgcgcctg aaaacctgac tcgctaactt cccgtttggc tggcaaaaaa
    18541 tcacctaaaa aactgggatg cggccagttg ctggttaagg tcatttcgct attacgcccg
    18601 gcaggcacca cagaaagatc gccggtaccg cttaaattca gggccatatt cagcttcagg
    18661 ttctgcttcc gccagtcccc ttcgggtaaa gggatatgca cgccctgccc gccttgctct
    18721 aacccggtgc cgggttcaat ggtcagcgcc gttccgttaa cttcaggcgc tttcaccaca
    18781 ccaataccac gcgcatcccc gacgctaatc acaataaagg gcttgcctaa gatgatattt
    18841 ggcgcgttga gctcgctaag acgcgaaaca tcgaaatcgg cttttagcgt taaatcactg
    18901 tgccagacct gaccggtata aatccctatc ttgcgttctt ccacgttctg attgccatca
    18961 accatcaatg actcaggtaa ccaaaaatgg ataaaacttc gtttccgctc cacggtttta
    19021 tcctcttcct gcaccgtata aagctcggtc acaggaatag cgataagcgg cccaacgagt
    19081 ttttgcggcc cgctggtact ttggcgaatc gcatcttcca catcgctacg gtaatcggca
    19141 cgttcgacaa tcacttgccg aatcagcatt atcggaatca acaacagcaa tactgcacca
    19201 aacaaggtgg tcattttcca gaacaggggg gatttcaaca tagtcttctc ctttgcaatg
    19261 gcagcaggat acgaaggcta tgtgggaaga atttgaagct atgtgaagtg acggtgaagt
    19321 cgaagcgagg ccagcacgcc accttcctgc acgttgcgca gcgtgacttc gccgttaaac
    19381 aaacgggcga cctcactgac gaacgccaac cccagaccgc tgcttttttg cccatttgca
    19441 cgcggcaaag agtaaaagcg ttcaaaaata cgtgacagcg cgtagtcagg aataccacta
    19501 ccggtatcca gcaccttaag ggcgacgtgt tcctgatcca cttcggcgct tagcgttatg
    19561 cgaccgctct cgggggtaaa atcgatggcg ttatccagta aattccccag cgcctgctcc
    19621 agtaacgccg gttcagcagc aacattaacc tcagtgggca taacatgtag agtgatgttt
    19681 ttttctgcca actgcacggt gcgcgcttcg ctgacgcggc gaaataatgc cgccacatca
    19741 acaacagtca gaacgacttc ctgacgattc tccagtcttg cctggcgtag taacgtttct
    19801 accaatgcct gcatacgcgc attttgcgtc agaatgttgt cggtaaaacg agccaccact
    19861 tccggtggcg gaccttcgcg taaaatttcc gccgcgccac gaatcgccgc cagtgggctt
    19921 tttagctcat gggttaacgc ataaacatac tgctcaatat agtttttccc ttccagcttc
    19981 acgcgcatac tttccagcgc ctgcgcaagc ttacgcaact cgctactgcc gagttcagga
    20041 agaggaacgg gcttattgtc agtgacggaa tcggcatagc gagtgagccg ggcaatggag
    20101 cggttgatcc accaaaccat gcctgcgcca atcaccagtg caatccccag caaaatggcg
    20161 ctggcccata aaattcgccg ctcgctacgc ttaatgaccg gagccatcgc cgcgttcggt
    20221 ttgcctacgc tcaaaacgcc aataagccgc gagccgtcca taatcggcgc ggcaacatac
    20281 atcaccgaac tttcgggatc ggcaggattt tgcaacgtgc tgcgcgcacc atactgacca
    20341 cgcaacgtta gccagacgtc attccagcgc gaataatcct gcccaacggc tttatttgcc
    20401 gaatcgaaca atactttacc ctgcgaatcg gtcatataga catgatattc attgcgcact
    20461 ttgttaatgc caccgatatt ggcgcgaaac gggcgatgtt gtagctgatt aaacgcctgc
    20521 gccagttgcc catgcgttgg gtccccagag agcaaatcgg gacgcgccag ctccgccagc
    20581 aacgttgcgg tatcgattaa cgtcccctcc gttgctcttc gcacgcccgg tttaacttct
    20641 ttgacaaaaa tagccagtac gaaccaggcc gccaccgcca ccagtaaaaa atagcccagc
    20701 agcaaccgca tgccgatacg cattacaggc ccctcaggct atatcccatg ccgcgatgag
    20761 tattaatcgg tgaaagatcg gggttgatgg cgcgcagctt ggcacgcaac gttttaatgt
    20821 gggtatcaat ggtgcgatcg taggtgtcct gcgcatcttc ccacacgcta tccattagtt
    20881 gctggcggga ccagacgcgg cctggcgact tgagtaacgt cttcagcaat aaaaactcat
    20941 accgcgtcag cgttaatggc gtgtcaaacc agctgatctg cgccgcgggt tcattcaatt
    21001 caaaatgacc aatacggatg acgggagacg gcgacgagaa cttcttcacc cgacgcagta
    21061 aggtgcgcac ccttgcgcac acttcgcggg gtgaaaacgg tttagccaca tagtcgtcag
    21121 cgccaatttc cagcccaagc aggcgatcga cctcttcact tcgggccgtc aggaacagta
    21181 caggtaacgc cggatggagc gccagtaact ggcggcataa ttcaaagccg ctgatatcag
    21241 gcaaaccaac atcgagaatc atgacgtcgg gagcctgctg gcgagcttta tccagcaccg
    21301 gcaagcctcg ctcaaagacc tcgacggcaa aaccttcctg ctgcaacatg tagaccagcg
    21361 tgtcggctat cccttgctca tcttccacta accagaccgt ttcccgttgc ataaatcgcc
    21421 tctgttattg ccgccacggc atgacaggaa ccgcgctaat cgcgtttttc ggcgaacctt
    21481 ctacaacttt gtcggagtaa gccagataag cgagcgcgtt gcgtttggcg tcataaaagc
    21541 gcacgacctg taacgactta aagaccaggg acgtgcgttt tttgaatact acctcgccct
    21601 gagctttgcc gtttttaata cgatccgaca gttcaatcgg cccgacttgc tgacaagaaa
    21661 tagccgcatc ggaggtatct tccgccagac ccaatccccc tttaataccc ccggttttcg
    21721 cccggctcac ataacaggtg acatttttca catcgggatc gtcaaaggct tccacaacaa
    21781 ttttgtgatc cgggccgatc attttaaata cggtgtcgac cgaaccaatc tcctctgcat
    21841 gagccaatgg ccccagcatt atcaggctta aagacaggat caaatgcttg tatttcatat
    21901 tgttaccatt tttatctatt acacgagcaa ttagttcgtc acggtaaata acatattgat
    21961 taagaacaaa atctcaatac ttttatttcc gtcaagccct aaaacatact ctactaagaa
    22021 aaaaacactg aatgctaaaa cagcaaaaaa tgctattatc caattacctg atgtcaggtg
    22081 ctcgttgttg aaaggatgag gatattttat ggatcaggcc ggcattattc gcgacctttt
    22141 aatctggctg gaaggtcatc tggatcagcc cctgtcgctc gacaatgtag cggcgaaagc
    22201 aggttattcc aagtggcact tacagagaat gtttaaagat gtcactggcc atgctattgg
    22261 cgcgtatatt cgtgctcgtc gtttgtcgaa atcggcggtc gcactacgcc tgactgcgcg
    22321 tccgattctg gacatcgcgc tgcaataccg cttcgactct caacagacat ttacccgcgc
    22381 attcaagaag cagtttgccc agactcctgc actttaccgc cgttctcctg aatggagcgc
    22441 ctttggtatt cgcccgccgc tacgtctggg tgaattcact atgccagagc acaaatttgt
    22501 caccctggaa gatacgccgc tgattggtgt tacccagagc tactcctgtt cgctggagca
    22561 aatctctgat ttccgccatg aaatgcgtta tcagttctgg cacgattttc tcggcaacgc
    22621 gccgaccatt ccgccggtgc tctacggcct gaatgaaacg cgtccgagtc aggataaaga
    22681 cgacgaacaa gaagtattct ataccaccgc gttagcccag gatcaggcag atggctatgt
    22741 actgacgggg catccggtga tgctgcaggg cggcgaatat gtgatgttta cctatgaagg
    22801 tctgggaacc ggcgtgcagg agtttatcct gacggtatat ggaacatgca tgccaatgct
    22861 taacctgacg cgccgtaaag gtcaggatat tgagcgatac tacccggcag aagatgccaa
    22921 agcaggagat cgcccaatta atctgcgctg tgaactgctg attccgatcc gtcgttaacg
    22981 ctgcaactca tctaatgcag gggcgtccag atgcgagatg tcccctgcgg tttcaacaac
    23041 ccagccagac gccagccaca ggctttcctg ataatccacg cgcgaaatag aacagttacg
    23101 cagacgtaag cggcgctctg cccatgctgg taatccgaga atcgtactca ccaggcatcc
    23161 cagtgcaata ccatgactca ccagcagcgg tcggcttccc tgcggtaagt cccggcaaga
    23221 ttccagtgcc gcattgacgc gatcgctgag ctcctgcatt gactcgcctt caggaatacg
    23281 cccgtcaacg gtgccattga ccagctgccg acgccagttc tcttcttctt cggtcagaga
    23341 atcgatatgt cttttttcca gcacacccat gtttaattca cgcaggcgag aatcaaagat
    23401 gatgtcgcag ccgcaggcct gggcgatgat ttccgccgta cgccgggtgc gtcctaaatc
    23461 gctactgatg atatgcgtaa tgccaagctc tttggcacgg gttgccacct gcatcgcctg
    23521 ttgctcacct ttggcggtca gcgggctgtc agactggccc tgaatacgtc gctcggcgtt
    23581 ccactgcgtt tcaccgtggc ggactaggta tacctgtaac atgctttttt tccgtatact
    23641 gctgtaacgt taattttctg agtaatgctg attatgcacc aagttgtctg tgcgaccacc
    23701 aatcccgcta aaattcaggc cattctgcag gcatttcacg agatcttcgg cgaaggatcc
    23761 tgccatattg catccgttgc cgtcgagagc ggtgtaccgg aacaaccctt tggcagtgag
    23821 gaaacgcgcg ctggcgcacg aaatcgggta gcaaatgccc gccgtttact tccagaggct
    23881 gatttttggg tggcgattga agctggcatc gatggtgaca gcactttcag ttgggtggtg
    23941 attgaaaacg ccagccagcg cggcgaggcg cgttcggcta ctttgccgtt gcctgcggtg
    24001 attctggaaa aagtgcgtga aggcgaggcg ctcggccccg tgatgtcgcg ttataccggt
    24061 attgatgaga ttggtcgtaa ggaaggggcg atcggcgtgt ttaccgccgg gaaactcacc
    24121 cgcgccagcg tctatcacca ggcggtgatc ctggcgctga gtccgtttca taatgccgtg
    24181 tatcaggcat tgtaggacgg ataaggcgtt cacgccgcat ccggcattcg gtgcacgatg
    24241 cctgatgcga cgct
//
LOCUS       DAZGJW010000065        23509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZGJW010000065 DAZGJW010000000
VERSION     DAZGJW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.065"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            19..735
                     /locus_tag="ACHKIK_002627"
     CDS             19..735
                     /locus_tag="ACHKIK_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO9083781.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            765..1265
                     /locus_tag="ACHKIK_002628"
     CDS             765..1265
                     /locus_tag="ACHKIK_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFO9083782.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQITKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1357..4041
                     /locus_tag="ACHKIK_002629"
     CDS             1357..4041
                     /locus_tag="ACHKIK_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFO9083783.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADISDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDGLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4038..5112
                     /locus_tag="ACHKIK_002630"
                     /pseudo
     CDS             4038..5112
                     /locus_tag="ACHKIK_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
     gene            complement(5200..6498)
                     /gene="exuT"
                     /locus_tag="ACHKIK_002631"
     CDS             complement(5200..6498)
                     /gene="exuT"
                     /locus_tag="ACHKIK_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO9083784.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6981..8393
                     /gene="uxaC"
                     /locus_tag="ACHKIK_002632"
     CDS             6981..8393
                     /gene="uxaC"
                     /locus_tag="ACHKIK_002632"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO9083785.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8408..9895
                     /gene="uxaA"
                     /locus_tag="ACHKIK_002633"
     CDS             8408..9895
                     /gene="uxaA"
                     /locus_tag="ACHKIK_002633"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO9083786.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9978..10529
                     /gene="ygjV"
                     /locus_tag="ACHKIK_002634"
     CDS             9978..10529
                     /gene="ygjV"
                     /locus_tag="ACHKIK_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO9083787.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10534..11778)
                     /gene="sstT"
                     /locus_tag="ACHKIK_002635"
     CDS             complement(10534..11778)
                     /gene="sstT"
                     /locus_tag="ACHKIK_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO9083788.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12177..13142)
                     /gene="alx"
                     /locus_tag="ACHKIK_002636"
     CDS             complement(12177..13142)
                     /gene="alx"
                     /locus_tag="ACHKIK_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO9083789.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(13425..14411)
                     /gene="ygjR"
                     /locus_tag="ACHKIK_002637"
     CDS             complement(13425..14411)
                     /gene="ygjR"
                     /locus_tag="ACHKIK_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO9083790.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14490..15169)
                     /gene="ygjQ"
                     /locus_tag="ACHKIK_002638"
                     /pseudo
     CDS             complement(14490..15169)
                     /gene="ygjQ"
                     /locus_tag="ACHKIK_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
     gene            complement(15246..15749)
                     /gene="ygjP"
                     /locus_tag="ACHKIK_002639"
     CDS             complement(15246..15749)
                     /gene="ygjP"
                     /locus_tag="ACHKIK_002639"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO9083791.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTPDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15834..16970
                     /gene="rlmG"
                     /locus_tag="ACHKIK_002640"
     CDS             15834..16970
                     /gene="rlmG"
                     /locus_tag="ACHKIK_002640"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO9083792.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSISDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            17161..17244
                     /locus_tag="ACHKIK_002641"
                     /pseudo
     CDS             17161..17244
                     /locus_tag="ACHKIK_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17241..17657
                     /gene="higA"
                     /locus_tag="ACHKIK_002642"
     CDS             17241..17657
                     /gene="higA"
                     /locus_tag="ACHKIK_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO9083793.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17702..19720)
                     /gene="fadH"
                     /locus_tag="ACHKIK_002643"
     CDS             complement(17702..19720)
                     /gene="fadH"
                     /locus_tag="ACHKIK_002643"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO9083794.1"
                     /translation="MSYPLLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVASSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHHVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20046..22397)
                     /gene="ygjK"
                     /locus_tag="ACHKIK_002644"
                     /pseudo
     CDS             complement(20046..22397)
                     /gene="ygjK"
                     /locus_tag="ACHKIK_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(22414..23484)
                     /gene="ygjJ"
                     /locus_tag="ACHKIK_002645"
     CDS             complement(22414..23484)
                     /gene="ygjJ"
                     /locus_tag="ACHKIK_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO9083795.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
BASE COUNT         5905 a         5987 c         6077 g         5540 t
ORIGIN      
        1 ttgtaagatc aaatttatat ggtggcgaaa attaatctct tatcacggct tacgccgctt
       61 ttattcgtgt tcgctccttt tttggcacag tcaaatatga ctgtttatcc tatggctgtt
      121 tccattaata gtcagggtga aggcaacgtt cgcgtaatat ctaaaagtaa tgaagttcag
      181 tacatcaagg cgacagtatt ccgtatcgat aatccatcga cgcctcagga aaatgaagtt
      241 gagattaaat ccggtgatgc aaatcatctg gttgttatgc cacctaaatt cgctttacca
      301 gccggtagta gcaaaaccgt acgttttgtt gcgatggaac cagagcaaaa agagaaaaat
      361 tatcgcgtta aatttgaagc ggttcccagt attgatgacg ttgccacaga taaaaaagat
      421 ctctctatgc agttaacagt taacttaatt tgggggattg ttgttagtgt tccacctcag
      481 caacctattg ctaagttaga agtaaatgct gcccaaaaat tagttaatgc agggaatcaa
      541 cgcttaaaga ttttaacgat cgcttattgt aaaaataata gcaaagaaaa ttgtaagata
      601 cagaccgtaa ataaaaacat cttccctggt caggaaagaa atcttgaaag catatctggc
      661 tacgacaaaa tcgttgtcaa atataataac tggatcacca aagataatgg cgagtttgaa
      721 ctggcggtcc attaattatc attgtaataa acgaaggaat agtaatgaaa aaggtatttg
      781 caaaatctct tctggtcgca gcgatgtttt ctgttgcagg ctccgcattg gcagtacaaa
      841 aggatattac tgtaacggcc aacgttgatg ccgctctgga tatgacacag accgataaca
      901 ccgcgttgcc gaaagcagta gaaatgcaat atctgccggg gcagggtctg caatcttacc
      961 agttgatgac caaaatctgg tctaacgacg ttaccaaaga tgtaaaaatg cagttagttt
     1021 ccccggcgca actggttcaa agcctggatg ctagcaagat tgttccgctg acagtaacct
     1081 ggggcggtga agaaattaaa gctgatgcgg caaccacttt taccgcaacc aaaatctttg
     1141 cttccgatgc gttaactaac ggttctctgg ctaaaaacct gatgtttgct caaatcacca
     1201 aaggtgttct ggagacaggc atctaccgtg gtgtagtaag catttatctg tctcaggata
     1261 tctaatccag ataacagaat taacacgtaa caaagggagg ggtagccctc ccttatttta
     1321 actgttttag tatttgttat ggatttcagg attgccatgg ataaaaaatt actggctctt
     1381 ttgatcctgg cgagtctcag cccggcagag gcgacattaa ccaaaattcc cgcagggttt
     1441 gaggttattg ctcagggaca gcaggagtat atcgaggttt atttttcagg gaaaagtctc
     1501 ggtaaatatt atgcaatggt taatcttgat accgtaacat ttcttgatcc agcaagtcta
     1561 tataacaagc tggaactgga tgtagacgat cagaaaatcg cgcatatagt gaaagaaaaa
     1621 ttatcgcagc cgctagctcg ccacggtgaa ttggcttgcg gttatgtacg tactgactca
     1681 gggtgtggtt ttctgaatac cgatacgctg gaaataatct ataatgatga agaaagttcg
     1741 gcaacgttgt ttattaatcc gcaatggaat tcagctttcg atgcgaagtc attatattta
     1801 aatccagaca aaaatactgt taatgctttt atacatcagc aagacatcaa tgttctggca
     1861 caggatgatt accaatcgtt gtctattcag ggaaacggtg cgctgggaat aacagaaaat
     1921 agttatattg gtgcacactg gaatttcaac ggttatgatg cagatgatgt cagtgacagt
     1981 aatgctgaca tcagcgatct ctattatcgt tatgattttt tacgtcgtta ttatgtgcag
     2041 gcgggtcgca tggacaaccg cacactattt aatgcacaag gcgggaactt tacctttaac
     2101 tttctgccac tcggtgcaat tgatgggatg cgtatcgggt cgacactgag ctatttaaac
     2161 caggcgcaaa gccagcaagg aaccccagta atggttctgc tttcgcgcaa ttctcgtgtt
     2221 gacgcttatc gtaatgagca acttttggga tcgttttatc tcaatagtgg ttcgcaattt
     2281 attgatacca gttcctttcc gccaggcagc tacagcgtgg cattaaaagt ctacgaaaat
     2341 aaccaactca cccgcaccga gcttgtgccg tttaccaaaa ccggcggtct gactgacgga
     2401 aatgcgcaat ggttcttaca ggcaggtaaa actacatcac aggtttctga tgatgaaagt
     2461 tcagcttatc agctaggagt acgcctgcca ttacatccgc aatatgagct ctacgcaggg
     2521 ctggcgaatg ccgatgatgt gagtgctttc gagttaggta ataactggac ggcagattta
     2581 ggcggggtgg ggaatcttgc aatcagcgcc agcgtgttcc gtaacgatga cggcggcaaa
     2641 ggtgatatgc aacaggccaa ctggagtaat ccgggatggc cgacgttggg cttttatcgg
     2701 accaactctg acggcgatgc ttgtacaacc gacagcagag agagctataa cgccttaagc
     2761 tgttatgaaa gtatttccgc gacggtttca cagaattttg tcggctggaa tatgatgctg
     2821 ggttataccc gcacacaaaa taacactgat gatagtttgc gttgggataa acagcagagc
     2881 tttgaaaata actatcttcg ccagacaaca gcgcaaagta tctctgaaac tgtacaactt
     2941 agcgcttccc gcgcttttgt gatgcgtgac tggattttga gtacgtcagt cggtgttttc
     3001 catcgtaatg acaacggtgg cgataacgac gacaacggct tgtacttatc gttttcgtta
     3061 tctgacacgc caacgatgga tagcaataac aacagccatt caacaaatgt gtctacggat
     3121 tatcgttata gcgaacagga tggcgatcaa acgtcatggc agttatcgca taccttttat
     3181 aacgattcat tcagccataa agaacttggc gtaaccgtcg gaggcctgaa caccgatacc
     3241 ataaacagcg cggttaacgg gcgttgggat gggcaatacg gaaatgtcta cgctaccgta
     3301 tctgatagtt atgaccgtaa gaatcatgat catctttcgg cctttacggg cacttatagc
     3361 tctacgctgg ctgtcagtcg ctatggcgtt aatttgggag ccagtggtac agacggtttg
     3421 ctgggtgcgg tactggtgga tgtgaaaggc ttctctgaac aggatgaaga gagtcaggat
     3481 ctgcaactcg aagcgcgggt ggcaggcagc cgaactttgc agcttggtca aagtgacagt
     3541 gtgttgttcc cttatcctgg atttcagtct ggttttgttg aggttaacga cagtagccag
     3601 ggcaatcagc aggggacaac aaacatcatt aacggtgcag ggaatcgtga attaatgttg
     3661 ttgcctggca agctgcgcta tcgcgaagtg tctgccagct ttaactacaa ctatatcggt
     3721 cgtttattac tgccggcagc ggtgaaaaaa ttcccgattg tggggctgaa cagcgccatg
     3781 ttactggtag ctgaagatgg cggatttaca cttgaaatta acggcagcga aaaagagctc
     3841 tatctgcttt ccgggcagca attccttaag tgtccactga gtgttgtaaa gaaacgcgcc
     3901 agcattcgtt acagcggaga tgttacttgt agtgtggtga cttattcaca attaccggag
     3961 tcgattcagg ttcaggcaca gttgaaacag cctaaattac gtggaaacgt tcagacggcg
     4021 caaagggagg ttgcaccatg agaaatcgat tgattgcggc gatattgggc ttgtttggtt
     4081 caggcagctc ctgacgtgac cagtgaaatt acgtatgatt tggcatctgg tagagcggat
     4141 tattacttct ggaaggatga agcgtcggca ggaaataatg gatatatgtg gtatgaatgt
     4201 tcgtatcctg acctccaaca aacctgtaca gctaatggaa atatatcgac agtacaaatc
     4261 tatttaactg aacaacgcag tgggatgcgt tggccggtaa aactcaaagg atttaaaaca
     4321 gccattgtaa gtagcgatga agcgccgcca ggatgcaagg ggggcaaagg gcttcagacg
     4381 aatcttaagg attctaatag atcttcatgt acagaagatg gtcaacatta ttatatatac
     4441 gatacaaagt ttcttacgct ctaccttgag cagacagaga tgaagaattt gccgattggt
     4501 ggcgtctgga aggggaaagt taaattacat tcgaacagcc cggcccagga ctatttcgca
     4561 aatattaccc tgaatacgct cgaccccaac catattgacg tgttcttccc ggagttcgcc
     4621 cacgccacgc caagggtgca gttagacttg catccaacag gaagcgttaa cggcagcaac
     4681 tacgcgcaag atctgaccat gttggacatg tgcctgtacg atggttttaa cggtaatgcc
     4741 atcagttatg aaatcatgct caaagatgaa gggcgaccag ctgcagggcg cagagacggt
     4801 tacttctcta tctatcgtca gggagggacc accaccgacg agggagaacg cattgattac
     4861 cgggtcaaaa tgtacaaccc ggaaaccggt gggcaaattg atgtgcgcaa taatgaaaat
     4921 atggtctgga acagcattaa cctgaaacgt gtgcgtccgg tggtactgcc cggtattcgc
     4981 tatgccgtga tgtgtgtgcc gacacccttg actctggcag tcgataaatt cagcgtgatg
     5041 gataaacagg ccgggtacta catgggcaaa ttgtcagtaa tctttacgcc ttccttgcca
     5101 accatcaatt aaccctgcaa accggacaaa cgcttttttc tatataaacg acgaagccgt
     5161 ctgataaagc ggcttcgtca ttacaactta agttaaacgt taatgttgcg gtgcgggatc
     5221 gttatgtgtt tcctgcgcca cctcaatcgc cggtttgttc tgcaacacgg tccagataac
     5281 cagcgcacct aacaggtcga acactgccag aactgcgaac agcgggctga agccgatggt
     5341 gtcagccagt gcaccgacaa ccagcgcaaa cagcgtactt gccagccatg cggacatccc
     5401 ggttaaaccg ttcgccgtcg ccacttcgtt acgaccgaac acgtcagaag agagcgtaat
     5461 cagcgcgcca gacagtgcct ggtgggcaaa accaccgata cacagcagca taattgcgac
     5521 atacgggttg gtgaacagac cgatcatacc cgggccaatc atcagcacag cacccagcgt
     5581 tacgaccatc ttacgggaaa cgatcaggtt cacaccaaac caacgctgga acagcggcgg
     5641 caggtaacca ccgaggatac aaccgaggtc agcaaacagc atcggcatcc aggcgaacat
     5701 cgcgatctct ttcaggttaa agccgtaaac tttaaacatg aacagcggga tccacgcgtt
     5761 aaaagtaccc caggccggtt ctgccaggaa acgcggcagc gcgatacccc agaactgacg
     5821 gttacgcagg atctgaccaa cggacatttt cttcgccgtg ctcacctggt gctgggcttc
     5881 ctgaccatta ataatatagt cgcgttcttc atcggtcaga tgcttctggt cgcgcggatg
     5941 tttatagaaa atcagccatg ccatcgccca gataaagctc aatgcaccgg agatgataaa
     6001 tgccatctgc cagctgtgca ttacgattgc ccataccacc agcggcggcg caatcatcgc
     6061 accaatcgaa gaacctacgt taaagtaacc taccgcgatg gaacgctctt tcgccgggaa
     6121 ccattcggag ctggctttca gacccgccgg gatcatcgct gcttccgcgg caccgactgc
     6181 accacgagca atcgccaggc caccccagct accagccagc gcggttgcac cacagaacac
     6241 ggcccacagc acagcaaaca ttgcataacc gattttcgta cccagcacat ccagtacata
     6301 acctgctacc ggttgcatga ccgtataagc agcagaatag gctgcgatga tataggaata
     6361 ctgttgggtg gagatgttta actcttccat cagagttggc gcagctgccg ccacagtgtt
     6421 acgcgtcagg taaccaagca cggtgccgag cgtcaccagt gcgatcatat accaacgtaa
     6481 ccctttaatt ttacgcatgt aaaacctcat cgttttgtta tatccgcagg aagcgcggcc
     6541 atcatcccgc cagaggctga agatgttagt tacgaccggg taaccgaaaa aaaaccggca
     6601 cgccccgaac gttgccatga aagtttcgtg ctaattcggc ttccgtaccg gttaacccga
     6661 ttcatctaac aaatgaatga atcggcaatt tgcattccgt cgacttatga tttgcgacgg
     6721 cagaaagata acttgtcata caactttaaa aggtgagagc catcacaaat gtgcgaatat
     6781 ttgtagggac attacctgac gacagcgagg ccagtactgg cgcggcctgc agagagattt
     6841 accactttga gagtaatttt tttaactacg tttattgatc taactcacga aaatatcttc
     6901 ggactctgga aattggtgtg ataactttgt cagcatcgca ccataagcaa gctagctcac
     6961 tcgttgagag gaagacgaaa atgactccgt ttatgactga agatttcctg ttagataccg
     7021 aatttgcccg ccgtctgtat cacgactacg caaaagacca gccgattttc gattaccatt
     7081 gccatttgcc gccgcagcag attgcggaag actatcgttt taaaaacctg tatgacatct
     7141 ggctgaaagg cgatcactac aaatggcgcg ctatgcgtac caacggtgtg gccgagcgtc
     7201 tgtgtaccgg tgatgcgtct gaccgtgaaa aatttgacgc ctgggcggcg actgttccgc
     7261 acactatcgg caacccgtta taccactgga cgcacctcga actgcgtcgt ccgtttggta
     7321 tcactggcaa attgctttct ccgtcaactg ccgatgaaat ctggaacgaa tgtaacgaac
     7381 tgctggcgca ggataacttc tccgcgcgcg gcatcatgca gcagatgaac gtgaaaatgg
     7441 tcggcaccac cgatgacccg atcgattctc tggagcatca cgcagagatc gccaaagacg
     7501 gctctttcac catcaaagtg ctgccgagct ggcgtccgga caaagccttc aacatcgaac
     7561 aggcgacctt taacgactac atggcgaagc tgggcgaagt ttccgatacc gacattcgcc
     7621 gctttgctga cctgcaaact gccttgacca aacgtctgga tcacttcgcc gctcacggct
     7681 gtaaagtgtc tgaccatgcg ctggacgtgg tgatgtttgc tgaggcgaac gaagcggaac
     7741 tggacagcat tctggcgcgc cgtctggctg gcgaaaccct gagcgagcac gaagtggcac
     7801 agttcaaaac tgcggtactg gtgttccttg gtgccgaata tgcacgtcgc ggctgggtac
     7861 agcagtacca cattggcgcg ctgcgtaata acaacctgcg tcagttcaaa ctgctggggc
     7921 cggatgtagg ctttgactcc atcaacgacc gtccgatggc agaagagttg tctaagctgc
     7981 tgagcaagca gaacgaagaa aacctgctgc cgaaaaccat tctgtactgc ctgaacccgc
     8041 gcgataacga agtgctgggc accatgatcg gtaacttcca gggcgaaggt atgccgggca
     8101 aaatgcagtt cggttccggc tggtggttta acgatcagaa agacggtatg gaacgtcaga
     8161 tgacccaact ggcgcagctc ggtttgctga gccgctttgt cggtatgctg actgacagcc
     8221 gtagcttcct gtcatacacc cgtcacgaat acttccgccg cattctgtgc cagatgatcg
     8281 gtcgctgggt ggaagcgggc gaagcaccgg cggacatcaa cctgctgggc gagatggtga
     8341 aaaatatttg ctttaacaat gcgcgtgact acttcgccat tgaactgaac taaggtctgg
     8401 ggttgatatg caatacatca agatccatgc gctggataac gtcgcggtcg ctttagcgga
     8461 tttggctgaa ggcacagaag tcagtgtcga taaccagact gttacgctgc gccaggatgt
     8521 tgctcgtgga cataaatttg cgttaacgga tatcgcaaaa ggggccaatg tcatcaaata
     8581 tggcctgccg attggttatg cattggcgga tattgcggcg ggggaacacg tccacgccca
     8641 caatacgcgc acgaatctga gcgatctgga tcagtatcgc tatcaacctg attttcagga
     8701 tctgcctgcg caagcggcag atcgtgaagt gcagatctat cgtcgcgcta acggcgatgt
     8761 cggggtgcgt aacgagctgt ggatcctgcc aaccgtgggc tgtgtcaacg gcatcgcgcg
     8821 gcaaatccag aaccgtttcc tgaaagagac caacaacgcc gaaggtaccg acggcgtgtt
     8881 cctcttcagc cacacctacg gctgctcaca actgggcgac gatcacatca acacccgcac
     8941 catgctgcaa aacatggtgc gccacccgaa cgcgggcgca gtgctggtga ttggtctggg
     9001 ctgtgaaaac aaccaggttg ccgcattccg tgaaacgttg ggcgatatcg atcctgaacg
     9061 cgttcatttc atgatctgcc aacagcagga tgatgagatc gaagccggga tcgagcattt
     9121 gcatcagctg tataacgtga tgcgcaacga taaacgcgag ccaggcaaac tcagcgaact
     9181 gaagtttggt ctggagtgcg gtggttctga cggtctttct ggtattactg ctaacccgat
     9241 gctggggcgt ttctctgact acgtgattgc taacggcggt actaccgtac tgaccgaagt
     9301 gccggagatg tttggcgcag agcagttgct gatggaccat tgccgcgacg aagcaacgtt
     9361 tgaaaaactg gtgaccatgg tcaacgactt caaacagtac tttattgccc atgaccagcc
     9421 gatctatgaa aacccatcgc cggggaacaa agcgggcggt atcaccacgc tggaagataa
     9481 atcccttggc tgtacccaga aagcgggttc cagcgtcgtg gttgacgtgc tgcgttacgg
     9541 cgagcgtctg aaaacgccgg ggctgaactt gttaagtgcg ccgggtaacg atgccgtagc
     9601 gaccagcgcc ctggcgggtg cgggctgcca tatggtgctg ttcagtactg gtcgtggcac
     9661 gccatacggc ggctttgtac cgacggtgaa aatcgctacc aacagtgaac tggcggcgaa
     9721 gaaaaaacac tggatcgact ttgacgcggg tcagctgatc cacggtaaag cgatgccgca
     9781 gttgctggaa gaatttatcg acaccatcgt tgagtttgcc aacggtaaac aaacctgcaa
     9841 cgagcgtaac gacttccgcg aactggcgat cttcaaaagc ggcgtaacgc tataaagtcg
     9901 aaatgagttg ttaataaaaa acggcgtttc ataatgtgaa gcgccgtttg cttttccata
     9961 cctgataagg atttatcatg accgcgtatt ggctggccca gggcgtgggt gtcatcgcct
    10021 ttctgattgg tatcacaaca tttttcaatc gtgacgaacg tcgcttcaaa aagcagcttt
    10081 cggtctatag cgccgttatt ggcgtacatt tttttcttct gggcacctat cccgctggtg
    10141 ccagtgccat ccttaatgcc attcgtacat tgattacctt acgcacgcgc agcttatggg
    10201 taatggcgat ttttattgtg ctgactggcg gaattggcct cgcgaagttc catcatcctg
    10261 tcgaactatt gccggttatc ggtacgattg tcagtacctg ggcgctgttc cgctgtaaag
    10321 ggctcaccat gcgctgcgtg atgtggtttt caacgtgttg ctgggtgatt cacaacttct
    10381 gggcggggtc gataggcggc acaatgattg agggtagttt tctgcttatg aatggcttga
    10441 atatcattcg tttctggcgg atgcagaaaa ggggaattga tccgtttaaa gtagagaaaa
    10501 cccctcccgc cgtagacgaa aggggttaaa caattaatta cgcagggcgc tatttgccag
    10561 acgatcgtct tccgcctggc aagctgccgc agtgaacagc acgtcagttg aagagttcag
    10621 cgcggtttcg caagagtcct gcaatacgcc gatgataaag ccgacggcaa ccacctgcat
    10681 ggcgatatcg ttcgaaatac cgaacatatt acaggccagc gggatcagca gcagagaacc
    10741 ccccgccacg ccggatgcgc cacaggcaca cagagaagcc accacgctca acagcagcgc
    10801 cgtgggcaga tcgaccggga tacccagcgt attaaccgca gccagcgtca acacggtaat
    10861 ggtgattgct gcgcccgcca tattgatggt ggcacccagc ggaatagaaa cggaataggt
    10921 atcgcgatcc agattcagct tttcacacag tgccatattc accggaatgt tcgccgcaga
    10981 gctgcgggtg aagaaggcat acacgccgct ttcgcgcagg cacagcagca ccagcgggaa
    11041 cgggttacga cgaattttcc accacaccag caatgggtta accaccagcg ccaccagtaa
    11101 catacagcca accagcacga ccagcagttg cgcgtagccc cacagtgtgg agaaaccggt
    11161 ggttgccagg gtagaagaaa ccagaccaaa aataccaatc ggtgcgaagc gaatgaccag
    11221 tttcaccata aaggtgacgg cattcgacat atcgttgacc aggtttttgg tggtctcgtt
    11281 accgtgacgc agtgcgaaac cgaggccaat tgcccacacc aggatcccga tgtagttacc
    11341 tttcagcagc gcgtcgatgg ggttggaaac catgctcatt accagcccgc gcatcacttc
    11401 tacaatgcct gacggtggcg aaatatcacc cgcgctactg gataagtgca gggtagaagg
    11461 gaaggcgaag ctgaaaacga ctgcggccag agcagcagag aaggtaccca gcagatagag
    11521 gaacaaaata gggcggatat tggttttctg cccgtgctgg tggttagcaa tagatgccat
    11581 caccagcatc aacaccagga tgggggcaac ggctttcagt gcgccgacga acaaagtacc
    11641 taacagacca acagcttccg ccgcgggttt tgagatccat gccagaagaa tccccagaac
    11701 aaggccgacc aggatttgtt ttaccaggct gccatgagcc agacgccgga atagccccgg
    11761 tgaacgttgc gtagtcattt ttcgatcctt tcattgtgtt gtcgcacatc cctggtgcgt
    11821 tctgtcgacg catctttcag gatgtaacaa agtgtttgca aggtcgagta taaggaaagt
    11881 tacagcttcg ggaagcgaaa aatgctggat tttaaagaga ggtcatattt tgcaacttaa
    11941 tggttacatt tatttgacat aaaaataaag ccaacgtgcg gcttccagac tgatgacaac
    12001 atcaaaaaag gactcggtca gtcccctcgc cccgtttggg gagagggtta gggtgagggg
    12061 aacaggccag catcggtgcg aaaaattacc ctcaccccga ccctctccct ggaagggaga
    12121 gggagaaaac ggtgctcatg ttaatgacag ggtacacggc ttaggcagat taaaaattat
    12181 ccaccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa cgtcatcacc
    12241 agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa gtcgacaatc
    12301 agcatcttga taccgataaa caccagaatc accgccaggc catatttgag catcgagaaa
    12361 cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat tgcaaacagg
    12421 tttgaggtca gcacaataaa cggatcagtg gtcacggcga agatagccgg aatgctatcc
    12481 acggcaaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag cggtgtggcg
    12541 tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc ggtcatgcgc
    12601 aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc atcttcatgg
    12661 gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata caggatccag
    12721 tcgaactgtg aaatcagcca gctgcctgtg aagatcatga tggtacgcag aacaatcgcc
    12781 ccgagcacgc catacaccag cacgcggcgt tgtaatgccg ccggaacaga gaaatagctg
    12841 aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat cagatagccc
    12901 gtgagaaagg ccagtgcctg tggatcggca acggcgcgac cttcagtttg caccagatac
    12961 caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga ccaggccgca
    13021 gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag gtcgatagcc
    13081 agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc gacagtattc
    13141 atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc cgctgctttt
    13201 tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga atacgtctgc
    13261 gtattccttt cgggttgccc ggtgaccgga tgtggttttt cacacatcgt aatgacgatc
    13321 gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc attcagccag
    13381 tcaatggtct gttacatacc ttttacactg ttttactttg gcaattatag ttttacgcta
    13441 tctgccggaa aaatcacccc agtctggcgg cggatctcgg tcagcagttt ggcggtgatg
    13501 cgactgaccg ccagccccgg atgatccacc agatgctcat ccaccagggt agcgaacagc
    13561 tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg catttggctg
    13621 ccacgcggca cgaagcacac tttctggcat tcagacagtt tttcaatcac cagcgatcct
    13681 gcttcgccct gaatctcgct cgccaggaca gaatcactga cttttgagtg ctgcaaggtg
    13741 acgctgaaat caccgtaatc catcaccacc acgccgtggg catctacacc gcttgccagc
    13801 aaactggcgg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc cgacgccagg
    13861 cagtaaaagc cgatatccat aattgaaccg ttagagaatg acggattaaa ggtgttggga
    13921 ttttcaccgt ccaggtaacg ttgatagcgc gaggaatact ggcaatagtt gaaaaagact
    13981 ttacgcaatt tgccgacttt cggcagcgcc tggcgcagca aatgaaagtt cggcaggcag
    14041 gcggttttaa atgcctcaaa cagcaccacc tgattttccc gcgcacaggc aatggcggca
    14101 tccacttccg ccagattcga cgccagtggt ttctcgcaaa tcacatgaat tttatggcta
    14161 aggaaaagtt gtgtctggga aaagtgcagg gcattcgggc tggcaatata caccgcgtca
    14221 atggcatcgc tttccgccat cgcttccagc gaggtaaaca gatgctcgac agaaaaatca
    14281 ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa cttgtattta
    14341 ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc aatcacagcg
    14401 aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg tagcacgcct
    14461 ttttcatctt cgacgtgcgc agatacgcat tattcttttg ccggacagcc atgctcacta
    14521 aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag gattttggcg
    14581 tcaatcacgg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa accgtgcttc
    14641 atattgaggt caggcgcgtt aaaaccgata gcgtcgatgc cgtactgttt tgccagccag
    14701 atggcgcgtt gattatgaaa ttcttgcgag ataatggtga tatggttttc accaaaaacc
    14761 tttttggctc gcactaccga atccagcgtt gagaatccgg catagtcgca gaagatcact
    14821 ttcgccggta cgcctttagc gatcaagcct gcatacctga ggcttcatcg taatttttac
    14881 gtccgttatc gccacttacc agtagccatt tcacttttcc ggcgtggtac aacgctgctg
    14941 cggtatcaat acgtcgggta aagtagcgat tacccggcct ggcccccagc aataagccca
    15001 cgttacgcgc cggaactgcg ttgacatcgc tccaggtcag ctgcttgctg gcgttgacca
    15061 taacgcgatc ggcaataaaa atggtcgctc ctgctataag caacaacaaa cagccaatct
    15121 taagagtccg gcgagaaaaa caaatacgga gaagaaggcg gggctgcatg gtcgttatcc
    15181 ttaacgaaag gggggaggtc gattttaggc gctaaagaaa gaaaagataa cgcctgacat
    15241 cattttcaga ttttgtcctg cccaagcgac aactgcgtta gccacagccg ggtgtcgaac
    15301 tcaagctggt ggtactgcgg ctccatatgg cagcacaact ggtaaaacgc tttgttatgc
    15361 tctttctcct tcaggtgcgc cagttcgtgc accacgatca tacgtaaaaa cggttccggc
    15421 gcgttgcgaa acacagtagc gacgcggatc tctgcttttg ctttcagctt gccgccctgc
    15481 acacgagaaa cagcggtatg taacccgagc gcgtttttta gcacatggat cttattgtca
    15541 tacatcactt tattgatcgg cggggcattg cgcagaaact gattttttag atccggagta
    15601 tattgccaga gggctttatc ggtggcgtag tcgtgtgttc ccggatagcg ttttgccagc
    15661 acatcgccca gacgctgttc gttaatcagc gttcgcacct gggaaagtag ctgctcggga
    15721 taaccctgaa gataagtaag attgctcata accgcccacg aaaaagagaa aaagggtata
    15781 ctcacgcacc cttttcaggg gcaacgtcga aattttatca ttcaggaggg ccgatgagcc
    15841 acttagacaa cggtttccgt tcactgacac tacaacgttt tccggcgacg gatgacgtta
    15901 acccgctaca ggcgtgggaa gcggcggatg aatatttgct gcaacagttg gacgacacag
    15961 aaatccgcgg cccggtgttg atcctgaatg atgcctttgg tgcgttaagc tgcgcgctgg
    16021 cggaacataa gccgtacagc attagcgact catacatcag tgaactggcg acgcgcgaga
    16081 atttacgcct caacgggatt gatgagtcga gcgtgaagtt tctcgacagc accgccgact
    16141 acccgcaaca gccgggtgtg gtgctgatca aagtgccgaa aacactggca ttgctggaac
    16201 aacaactgcg tgcgctgcgc aaagtggtca cgtcggatac acgtattatt gccggtgcca
    16261 aagcccgtga cattcacact tccacgctgg aactgttcga aaaagtgctc ggcccgacca
    16321 ccaccacgct ggcatggaaa aaagcgcgcc tgattaattg cacgttcaat gaaccgccgc
    16381 tggccgatgc gccgcagacc gttagctgga agctggaagg tactgactgg actatccata
    16441 accatgcgaa tgtcttctcc cgcaccgggc ttgatatcgg cgcgcgcttc tttatgcaac
    16501 atctgccaga gaatctcgaa ggggagattg tcgatctcgg ttgcggtaat ggcgttattg
    16561 gtctgacgct gcttgataaa aacccgcagg cgaaagtggt gtttgtcgat gaatcgccga
    16621 tggcggttgc ttccagccgt ttgaacgttg aaaccaacat gccagaggcg ttggatcgca
    16681 gcgagtttat gatcaacaac gcgctctccg gcgtggagcc tttccgcttt aatgctgtgc
    16741 tctgcaaccc gccgtttcac caacaacatg cgctgaccga taacgtcgcc tgggagatgt
    16801 tccaccatgc ccgccgctgc ctgaaaatca acggcgagct gtatattgtt gccaaccgtc
    16861 acctggatta cttccataaa ctgaagaaga ttttcggcaa ctgcaccacc atcgccacga
    16921 ataataaatt tgtggtgctg aaagcagtga agctggggcg tcgtcggtaa gattgggagt
    16981 tacctgccgg atgcggcgtt aacgccttat ccggcctacg ttcagcacgt tactcccagg
    17041 ccggataaga cgcggcaagc gtcgcatccg gcaatgtctg cccagttctg gaatcgcgct
    17101 tccataaaac aaaaaatccc tgtcaccatc ctctacattc tcttgtttag cgttgttcaa
    17161 cgtttatgct acatacgtga agttatgacg cataaagaat acgatttctt taccgctgtt
    17221 catcgtacta aggggaaaaa atgattgcga ttgccgacat cttgcaagca ggagaaaagc
    17281 taactgctgt ggcacctttt ctggcgggta ttcagaacga ggaacaatac acccaggcgc
    17341 tggaactggt agatcatctg ctgctcaacg atcctgaaaa ccccttgctg gatctggtgt
    17401 gtgcaaaaat aaccgcgtgg gaagaatcag cgcccgaatt tgcggaattt aatgccatgg
    17461 ctcaagccat gcctggcggt atagccgtga ttcgtaccct tatggatcaa tatggtttaa
    17521 ccctttccga tctgccggaa attggcagta aatctatggt gtcacgcgtt ttgagcggga
    17581 agaggaaatt aacgctggaa cacgctaaaa aattggcaac gcgattcggc atttctcccg
    17641 ccttgtttat tgattaacgc gacgtgccgg atgcgttgca tccggcgctt caaataacga
    17701 tttaaatctc cagcgccagc cgtgttccct gggcaattgc ccgtcgtgcg tccagctcca
    17761 tagccacatc gcagccgcca attaaatgca cagttttccc gctatcaatc agcggttgcg
    17821 ccagcgcgcg gtttggctct tgccctgcgc agatcaccac attgtccact gctaatacct
    17881 gcgtttcgcc gttgatcacc acatgcagcc cgtcatcgtc aatcttctga taacttacgc
    17941 ctgggatcat tttcacaccc cgcgagagca gggtggtgcg atggatccag ccggtggttt
    18001 tgcctaaccc ctgtcccggt ttgctggctt tgcgctggag catcacaatt tgccgtgggc
    18061 tacgcgggat ctgcattccc tgcgggctta agccaccagc ctgttgtagg ctgctgtcga
    18121 tcccccactc attacagaac ctggcgatat tctggctggt ggattcgccc ggctgactta
    18181 aatacatcgc cgtatcaaaa ccaatcccgc cacaaccgat gatggcaacc ttgttgccta
    18241 ccggcgcttt gtcgcgcagt acatcgagat aactcaatac cttcggatga tcgatcccgt
    18301 cgataggcgg aacgcgcggc acgatcccac tggcgaggat cgtttcatcg aacgcctgta
    18361 actgatccgc cgtcacggtg tgattgagtt tcagcgtcac gcccgtcact tcgatcatcc
    18421 ggtggtaata gcgcagcgtt tcgtaaaact cctctttgcc ggggatctgt ttggcgatat
    18481 taaactgccc gccgatctcg ctgtgggcat caaacaatgt tacatgatgc ccacgcgccg
    18541 ccgcgttaat ggcaaacgcc agcccagcag gtcccgcacc gaccaccgcc agattttttt
    18601 tctgcacggc aggaaggatt ggcattttgg tttcgtggca ggcgcgagga ttcaccaggc
    18661 acgaggtgac tttgccaacg aagatctgat cgaggcaggc ctgattgcag ccaatgcagg
    18721 tgttgatctc atcggctcgc cccgattgcg cttttgacag cagttccgca tcagcaagaa
    18781 acggtcgtgc catcgatacc atatcggcat cgccgcgcga gagaatgtcg tcggcaacct
    18841 gcggatcgtt aatccggttg gtggtcacca gcggcagcga aacgtggcct ttcagtttgc
    18901 gcgtgaccca agtaaatgcg ccgcgcggca cgggcgtggc aatggtcgga atacgtgctt
    18961 catgccagcc aatgccggtg ttgataatgg tcgcgcccgc cgcttcaatg gcctgcgcca
    19021 gctctaccgt ttcggcaaaa gtcccgccgt cttcgaccag gtcgagcatc gacagtcggt
    19081 agataataat gaagtcgttg ccgacgcgtt cgcgcaccgc acgcactact tctacggcaa
    19141 atcgcatccg gttgcggtaa tcgccgcccc actggtcact acgctgattg gtgcgcagcg
    19201 tcagaaattc gttgatcaaa tacccttcgg accccattac ctctacaccg tcgtatcctg
    19261 cctcccgtgc cagttgcgcg cagtgggcga aatcgtcgat cagttgcagg atctcttcat
    19321 gggtgagttc gtgaggaacg aaacggttga tgggggcctg caatgcggag gaagcgacca
    19381 gatgcggttg gtagctgtag cgcccggtat gcaaaatttg cagggctatt ttgccgcctt
    19441 cctgatgtac cgcttcggta atggtgcgat ggtgtgggat ctggctggcg tcgttgagca
    19501 ttgcgccacc ttccatgcca acgcctgtta aatctggtgc aatgccgccg ctgacaatca
    19561 gcgccacgcc gtgacgggcg cgttcggcat aaaacgctgc cagccgctcg gcaccgtccg
    19621 ggtattcctc cagcccggtg tgcattgagc ccatcaacac gcggtttttt aacgtggtaa
    19681 aacctaaatc cagcggggcg aacagcaacg ggtagctcat aaaattgtcc agtatgtaaa
    19741 ataattgtta tgtggtcgga tgagttctaa tttagccgtc gccggagaaa agggaaaagg
    19801 ggagtgcggg gtttgtgatg ggattcaaaa agaatacgcc cggtggtaac gccggatgcg
    19861 acgcttgccg cgttttatcc ggcctggggg attgcacatg tagggcggat aaggcgttca
    19921 cgccgcatcc gccagtggca cggtgcagat gccggatgcg acgcttgtcg cgttttatcc
    19981 ggccaggagg attgcacatg tagggcggat aaggcgttta cgccgcatcc gccagcagtt
    20041 gcaaattatt gcttacggaa aaaatcgtta tacaacatat acaaatgcgc ggcactccag
    20101 gagaaatttg gtgcgccttg ctgtgcgcca gtcagcgggt tgtaattctc ctgaattggg
    20161 ccatcggcgg ttaatccttt ggcgtgacgg aagaacgtat ccgccagttt cagggcatca
    20221 tcacgataac cgtaacgctc catccctttc agaccaaacc agaactgatc cacccatacg
    20281 cgcccgcgcc agtagatatc agcgccaaaa gccggattgg ttaacgccgc cgttcccagc
    20341 gggacaaagg tgttgaactc tttaggatcg agcatcacct tcaccaccgc gtcggcgttg
    20401 gcctgcgttg ccgcaccgtt aaacagcggc gaccagcctt ccggcccttt accgcgctcg
    20461 acaatcggtt tgcccgcgca gccgttcgcc agcggtttat cttcaatacg cacgtcatag
    20521 tagaactggg tagccgggtc gaacatacag gtgtttatgt agtctgcgag ctgctgtgcc
    20581 aactggcgat agcgtttggc ctcttccggc ttaccgagga tggttgccat ctccgccaga
    20641 taatggttat cgctgtacat atagctggcc tgatccaccg actcctgcaa tagcgagtag
    20701 cccagcaacg ttccgtcctg actgcggttt tcggcgaatt tcaccgtcca gtcgctacgt
    20761 ttgccgccat tagcgacata tttatccaac tgttctttgt cgataaaacc aaagacggcg
    20821 gcatcatcac gccccgattc ccacgacgca gcgacctgtg ccggaatttc cagactgtcg
    20881 tactggcctt tctccaccac gcgggcgtag ttgttcagcc cagactgcgt ctcttctttg
    20941 ttgcctttct tcaccgtaaa cagcatctcg ccgctctcag tgttgtgggc tttgtcgcgg
    21001 gtcgcgccat attacggcac gccgttgccg ttgtgatcgc ggttacgtaa ccaccagtcg
    21061 tgataggcca ccagtttcgg gtacatctct gccaaccagg ctttatcctg ggtgacgttg
    21121 tacacttcca tcaccgacca ggcggcaagg ctgggtttgg tattacgttc gttccagttg
    21181 ccgccatcgc caccacgctc ggggctaagg ttccacgcta tcaggtcggg gacaaagccc
    21241 acatcctgcg gacgcacgct atcgccaggc tggatctgcc aggagaagac cgcgcggata
    21301 ttctctttgg cgatgtccgg attgaaatgc gccatcgcaa acgcctgctt ccaggtatcc
    21361 cacggccagg tctgattgcc ggagaaccag cgcccggtca ccgacggtgt aacggtgtta
    21421 aatttcaccg caccgcccgg tgagcgccag ttaccgttga gcgtttcgat cgctttcacc
    21481 gcgacgcgcg tctgttccgg cgtcgcatcc ggattggtta accctttctt cagatactct
    21541 tcccagcgtt gctgcgaggc ggtgagataa aacgccggac gcgccagaat atcgcggatc
    21601 tgcatttgct ctttgctaac ttcctgagag gtcagcagat gggaataggt ggtatagagc
    21661 gtcgtcgaac cgttgatatg tgccttactg gtaaagcgat tgccgttgat ttcagtctgc
    21721 accggcaggg atttatgcac ctgatattcc gattcgccgg aggtcagcag atcccaggtg
    21781 gcgcgcactt tgccaaaggt gactttcagg ccatcacggg tggcgctgat tttgcgctgg
    21841 tagtcagggt attcgccagc aatggtttta tcggaaagcg gtttcccttc tttcgcttcc
    21901 agtttttcca gcagttcgcc gtcccacacc agatccagcg gtttattgct ggtgattttg
    21961 gtttccagta gtgacgtgcg cggcgtggcg aagcgcagag tcatttcgac ttgcacatct
    22021 tttgctgtca gtttttgcac cagcgcaccg ggaatactgt atgcctccag cgtaaagtcg
    22081 actttcttgc cgtcctgcca gacggtcagg cggtcgaaat tgctggccat aaagttgatg
    22141 tactcttccg tcagcagcgc aacgcccgga aagccgccca tggtgttagg gccgtctggc
    22201 aacagatgac catgccaggc tccgagatcg aaaaacggat taaaacgctg gtgatcgtcg
    22261 taatcgtaat ccttcatgta ctgcggcgcg ccagtacggt taatcacgtt tttataattg
    22321 tcggcgttag cggcatgggc ggaaaaactt atcagcagag cgcaggttac tggcgttaaa
    22381 atagttttta ttttcatgaa attacagtcc ttattaccag tagaaatatt gcatcaggaa
    22441 gatttggtcg ctgggatcgt gagaggcatt tttcatgtag aagcggctat caaaagccgt
    22501 ggcgaaacgt ccgccgccgt attcgtacca gataccaaac tgcatgaaag aggtggtttc
    22561 atcgtcagcg tggtcagggc gatgtttggc gtaggtgtaa gcggtggaga gataaacgcc
    22621 ctttaacgct tcatacatgg cgctcaccat catcccactt tcacttcccg gtacgtccag
    22681 cccgtcggcg cgtccggtat ggtgccaggc gcgagcggca aagttaggag ttagcaggcc
    22741 cgtgagatag agctgtcctg tgccgtcggt gatttccaga ccgttcatcc aggtgatgcc
    22801 ttcggcaagg tcatactgga tgtaaccgtt catcattgcc ggataggtgt atttgtcgtc
    22861 atagcgatcg tacttgccaa aatgtagcca ggctttgctt tcgtcatgat gtccggcagg
    22921 tgtggcggtg acgctataac gaaagttgcc gcttaagttc tgcaccttca gcgcggtcat
    22981 aatgtcgcgg gtattgggaa tgacgtagcc cagatcgggg gtgaagtcgc cccaccattg
    23041 caggtcatcc agcgaggaat cctgacgcag actgagcatg atttcggtgc catcattggt
    23101 acgataaccg ccatagaaac ggttaattcc gccctcgaaa ccaccccagc tatggtctgg
    23161 cacccaggca ttgccgtcgt ggtcggcctg cacggtccag ccttcgccgt aaatcaggcc
    23221 gaaccaggag ccgtacttca tctccaggcc accttcaata taggtaccgt cgctgtattt
    23281 atgtttattc tcaccctcta aatccatata tccaccaacc cctaattcgc cgtaaaaacg
    23341 aaatgaagga gaagatgtcg aggtaattgc aggtgttttt gtgtcgtcat gttctgccgg
    23401 acgtgcttct tctcctgccg cagaaaaggc gtagcaaaac ggcagagcaa gtaatgctct
    23461 gcatggtgca gtgattaatt tcattgtgtt aatccatgag ctaaagaaa
//
LOCUS       DAZGJW010000066        23264 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZGJW010000066 DAZGJW010000000
VERSION     DAZGJW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.066"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            165..1529
                     /locus_tag="ACHKIK_002646"
     CDS             165..1529
                     /locus_tag="ACHKIK_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9083796.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(1657..2148)
                     /gene="yajQ"
                     /locus_tag="ACHKIK_002647"
     CDS             complement(1657..2148)
                     /gene="yajQ"
                     /locus_tag="ACHKIK_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO9083797.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2316..3227
                     /gene="panE"
                     /locus_tag="ACHKIK_002648"
     CDS             2316..3227
                     /gene="panE"
                     /locus_tag="ACHKIK_002648"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO9083798.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3190..3780
                     /gene="yajL"
                     /locus_tag="ACHKIK_002649"
     CDS             3190..3780
                     /gene="yajL"
                     /locus_tag="ACHKIK_002649"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO9083799.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3834..5282)
                     /gene="thiI"
                     /locus_tag="ACHKIK_002650"
     CDS             complement(3834..5282)
                     /gene="thiI"
                     /locus_tag="ACHKIK_002650"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO9083800.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5488..5730
                     /gene="xseB"
                     /locus_tag="ACHKIK_002651"
     CDS             5488..5730
                     /gene="xseB"
                     /locus_tag="ACHKIK_002651"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO9083801.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5730..6629
                     /gene="ispA"
                     /locus_tag="ACHKIK_002652"
     CDS             5730..6629
                     /gene="ispA"
                     /locus_tag="ACHKIK_002652"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO9083802.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6654..8516
                     /gene="dxs"
                     /locus_tag="ACHKIK_002653"
     CDS             6654..8516
                     /gene="dxs"
                     /locus_tag="ACHKIK_002653"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO9083803.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8696..9670
                     /gene="yajO"
                     /locus_tag="ACHKIK_002654"
     CDS             8696..9670
                     /gene="yajO"
                     /locus_tag="ACHKIK_002654"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO9083804.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFVSMQDHYNLIYREEESEMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9724..10242)
                     /gene="pgpA"
                     /locus_tag="ACHKIK_002655"
     CDS             complement(9724..10242)
                     /gene="pgpA"
                     /locus_tag="ACHKIK_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO9083805.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10220..11197)
                     /gene="thiL"
                     /locus_tag="ACHKIK_002656"
     CDS             complement(10220..11197)
                     /gene="thiL"
                     /locus_tag="ACHKIK_002656"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO9083806.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11275..11694)
                     /gene="nusB"
                     /locus_tag="ACHKIK_002657"
     CDS             complement(11275..11694)
                     /gene="nusB"
                     /locus_tag="ACHKIK_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO9083807.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11714..12184)
                     /gene="ribE"
                     /locus_tag="ACHKIK_002658"
     CDS             complement(11714..12184)
                     /gene="ribE"
                     /locus_tag="ACHKIK_002658"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO9083808.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12273..13376)
                     /gene="ribD"
                     /locus_tag="ACHKIK_002659"
     CDS             complement(12273..13376)
                     /gene="ribD"
                     /locus_tag="ACHKIK_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO9083809.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13380..13829)
                     /gene="nrdR"
                     /locus_tag="ACHKIK_002660"
     CDS             complement(13380..13829)
                     /gene="nrdR"
                     /locus_tag="ACHKIK_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO9083810.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13980..14519
                     /locus_tag="ACHKIK_002661"
     CDS             13980..14519
                     /locus_tag="ACHKIK_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO9083811.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            14818..15702
                     /gene="tsx"
                     /locus_tag="ACHKIK_002662"
     CDS             14818..15702
                     /gene="tsx"
                     /locus_tag="ACHKIK_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO9083812.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15879..16226)
                     /gene="yajD"
                     /locus_tag="ACHKIK_002663"
     CDS             complement(15879..16226)
                     /gene="yajD"
                     /locus_tag="ACHKIK_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO9083813.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16355..17326)
                     /gene="secF"
                     /locus_tag="ACHKIK_002664"
     CDS             complement(16355..17326)
                     /gene="secF"
                     /locus_tag="ACHKIK_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO9083814.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17337..19184)
                     /gene="secD"
                     /locus_tag="ACHKIK_002665"
     CDS             complement(17337..19184)
                     /gene="secD"
                     /locus_tag="ACHKIK_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO9083815.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19212..19544)
                     /gene="yajC"
                     /locus_tag="ACHKIK_002666"
     CDS             complement(19212..19544)
                     /gene="yajC"
                     /locus_tag="ACHKIK_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO9083816.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19567..20694)
                     /gene="tgt"
                     /locus_tag="ACHKIK_002667"
     CDS             complement(19567..20694)
                     /gene="tgt"
                     /locus_tag="ACHKIK_002667"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO9083817.1"
                     /translation="MKFELDTTDGRARRGRLVFDCGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20750..21820)
                     /gene="queA"
                     /locus_tag="ACHKIK_002668"
     CDS             complement(20750..21820)
                     /gene="queA"
                     /locus_tag="ACHKIK_002668"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO9083818.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21913..22494
                     /gene="acpH"
                     /locus_tag="ACHKIK_002669"
     CDS             21913..22494
                     /gene="acpH"
                     /locus_tag="ACHKIK_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO9083819.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22499..>23264)
                     /locus_tag="ACHKIK_002670"
     CDS             complement(22499..>23264)
                     /locus_tag="ACHKIK_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFO9083820.1"
                     /translation="MQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAMNYR
                     GFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDTARF
                     KTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQDTAL
                     FALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEVVLP
                     ASPLLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
BASE COUNT         5643 a         6218 c         5913 g         5490 t
ORIGIN      
        1 tttaaaattc attttttcaa catatagagc attcatttta tgaatgcaaa aaaacatacc
       61 tgacacacaa attactctcc tctgtaatat tttctcatta acactctgtt tacccaacat
      121 gcgctccgca ctacactatg tgggcatttt tttaaggggt ggtaatgaac gattataaaa
      181 tgacgccagg tgagaggcgc gcgacctggg gtttagggac cgtattctcg ttgcgcatgc
      241 tgggcatgtt tatggttctg ccggttctga ccacgtacgg catggctctg caaggtgcca
      301 gcgaagcatt aatcggtatt gccattggta tttatggtct gactcaggcc gtttttcaga
      361 ttccgtttgg cctgctttcc gaccgcattg gtcgcaaacc attaattgtc ggtgggctgg
      421 cggtgtttgc cgccggtagc gttatcgctg cgctttccga ctccatctgg ggaattattc
      481 tgggccgggc gctacaaggc tctggagcaa ttgccgccgc cgttatggcg ctgctttccg
      541 atctcacgcg cgaacaaaac cgcaccaaag cgatggcgtt tatcggcgtg agctttggca
      601 ttacctttgc cattgcgatg gtgcttggcc cgatcatcac tcacaaactt gggctgcacg
      661 cgctgttctg gatgatcgct attctggcaa caaccggcat tgcgttgacc atttgggttg
      721 tacccaacag tagcactcac gtacttaatc gtgagtccgg aatggtgaaa ggcagtttca
      781 gtaaagtgct ggcggaaccg cggctgctaa aactcaactt tggcattatg tgtctgcaca
      841 tgcttctgat gtctacgttt gttgccctgc ccggacagct agctgatgcg gggttcccgg
      901 cggctgaaca ctggaaggtc tatctggcga cgatgttaat cgcctttggc tcggtcgtgc
      961 ctttcattat ctacgctgaa gttaagcgca aaatgaagca agtctttgtc ttctgcgtcg
     1021 ggttgatcgt ggttgcggaa attgtgttgt ggaacgccca aacgcagttc tggcaactgg
     1081 tggtcggcgt gcagcttttc tttgtggcgt ttaatttgat ggaagccctt ctgccctcac
     1141 ttatcagtaa agagtcgcca gcaggttaca aaggtacggc gatgggtgtc tactccacca
     1201 gccagtttct tggcgtggcg attggcggtt cgctgggcgg ctggattgac ggcatgtttg
     1261 acggtcaggg ggtatttctc gctggcgcaa tgctggccgc agtgtggctg gcagtcgcca
     1321 gtaccatgaa agaaccgccg tatgtcagca gtttgcgcat tgaaatcccg gcggacattg
     1381 ccgcaaacga agcgttaaaa gtgcgtttgc tggaaactga aggcgtcaaa gaagtgttga
     1441 ttgcagaaga agaacattca gcttatgtga aaatcgacag caaagtgacg aatcgctttg
     1501 agatagaaca ggcaattcgt caggcataaa aaacgcggcg aattgcctgc tgcgctacgc
     1561 ttatcaggcc tacaaagttt ctgaattaca aacctttgta ggccgaatca ggcgttcatg
     1621 ccgcatctga catgaacaaa acgcacatag tcgcgattaa tcgcggaagt ttttgaactg
     1681 gaacggctga ccgagatcgc caccacgtac catcgccatg acagcctgca aatcatcacg
     1741 agatttgccc gttacgcgga tttcatcgcc ctgaatttgc gcctgcactt tcagtttgct
     1801 gtctttgatc atcttgacga ttttcttctg ggtcgcgctc tcaatgccct gtttcagttt
     1861 cgcttccaca aaccaggttt taccgctatg aacgatattc tccggtacat ccagcgaact
     1921 gccttcaatg ccgcgcttca gcagcttggc acgcagaata tccagcaact gattgacctg
     1981 gaaatcggac tcgctcaaca ctttgatggt tttgctggcg tcgttcagct caaatgaggc
     2041 ttcaacgtta cggaagtcaa aacgggactc cacttcgcgg ctcgcgttat cgaccgcgtt
     2101 acgtgcttcc tgaagatcaa cttcagagac aatatcgaaa gatggcatct tttcctctcc
     2161 cttcattttt gatgcgaagc ataatacccg caaagttaaa taaccgacag tcctgcacca
     2221 ggacgtatcc cttaattgtg catagtcgca acagcatgca caattaaggg atagccttat
     2281 tgaacacccg gagtggttgc gggtgaggag gaacaatgaa aattaccgta ttgggatgcg
     2341 gtgccttagg gcaattatgg cttacagcac tttgcaaaca gggtcatgaa gttcagggct
     2401 ggctgcgcgt accgcaacct tattgtagcg tgaatctggt tgagacagat ggttcgatat
     2461 ttaacgaatc gctgaccgcc aacgatcccg attttcttgc caccagcgat ctgctcctgg
     2521 tgacgctgaa agcatggcag gtttacgatg ccgtcaaaag cctcgcgtcc acactgcctg
     2581 taaccacgcc aatactgtta attcacaacg gcatgggcac catcgaagag ttgcaaaaca
     2641 ttcagcagcc attactgatg ggcaccacca cccatgccgc ccgccgcgac ggcaatgtca
     2701 ttattcatgt ggcaaacgga atcacgcata ttggtccggc acggcaacag gatggcgatt
     2761 acagttatct ggcggatatt ttgcaaaccg tgttgcctga cgttgcctgg cataacaata
     2821 ttcgcgccga gctgtggcgc aagctggccg tcaactgtgt gattaatcca ctgaccgcca
     2881 tctggaattg cccgaatggt gaattacgtc atcatccgca agaaattatg cagatatgcg
     2941 aagaagtcgc ggcagtgatc gagcgcgaag ggcatcatac ttcagcagaa gatttgcgtg
     3001 attacgtgat gcaggtgatt gatgccacag cggaaaatat ctcgtcgatg ttgcaggata
     3061 tccgcgcgct gcgccacacc gaaatcgact atatcaatgg ttttctctta cgccgcgccc
     3121 gcgcgcatgg gattgccgta ccggaaaaca cccgcctgtt tgaaatggta aaaagaaagg
     3181 agagtgaata tgagcgcatc ggcactggtt tgcctcgccc ctggtagtga agagactgaa
     3241 gccgtcacca ctatcgatct gctggttcgc ggcggtatca aagtcaccac tgccagcgtc
     3301 gccagcgatg gcaacctggc cattacctgc tcgcgcggcg tgaagctgct ggcggatgcg
     3361 ccgctggtcg aggtggctga tggcgaatat gacgtgatcg tactgcctgg tggcattaaa
     3421 ggcgcagagt gttttcgcga tagtaccctg ctggttgaaa ccgttaaaca gttccaccgt
     3481 tccgggcgta tcgtcgcggc tatttgcgcc gcgccagcca ccgtgctggt gccgcacgat
     3541 atcttcccga ttggtaatat gaccggcttc ccgacgctga aagacaaaat tcccgctgaa
     3601 caatggcagg acaagcgcgt cgtctgggat gcacgggtaa aattgctgac cagccagggg
     3661 ccggggacag ctatcgactt tggtctgaaa attatcgacc tgctggttgg gcgcgagaaa
     3721 gcccatgaag tggcatcaca actggtgatg gcggcaggga tttataatta ttacgagtag
     3781 tgtcggatgc ggtaagcgtc gcatctgacc agatgcgacg ttacaaccac aaattacggg
     3841 cgatatacct tcacattgtt aaagccctgc tcgcgcagat agagcgcctg cagacggctc
     3901 atcaccccgc gctcacacca cagtagccag gttctgttct ggtcgagatc gccaaacttg
     3961 gtgctcagtt tatagaacgg cagagaaact acatcaatcc cttcgacttt cagtggctta
     4021 tcttcctgtt catcgataga acggatatcg aggatcacgt cgttcgggcc gaagccattg
     4081 acggtttcca cttccaccac ttcctgctcg gtctgctggg cgatttcgcg gatatcaacg
     4141 ttattcgctt cttcaaccac tttatcgaga atgctgaagt cgaatttctc ttcttccgct
     4201 tcaatcttcg acttaaccgc tttcaccgtc gggcttttgg agataacacc gcaatattcc
     4261 ggcatcgtgc gagcaaagtc ttcggtgcca atctggcgag ccaggttgat gatgtgctct
     4321 ttgtcgtaag aaatcagcgg acgcaggatc agcgtatcgg agacgttatc aatcaggcgc
     4381 aggttggtca gcgtctgact ggacacctgg ccgagcgctt cgccggtgac cagcgcctgt
     4441 acgccgtaac gttcagccac tttagacgcg gcacgcacca tcatacgttt gaggataacg
     4501 cccatctgac cgtcgtcgat tttctcgaga atttccccga cgaccggttc gaaattaata
     4561 gcgacaaaac gcacgcggtg ggagctgcca aaacggttcc acagataatg cgccacctga
     4621 cgcacgccaa tttcatgcgc cgcgccgcca aggttaaaga aacagtaatg cacgcggcag
     4681 ccgcgacgca tcaacatata actggaaaca ccggagtcga aaccaccgga aatgagcgac
     4741 agcacatctt cctgggtgcc gatcgggaaa ccgccaatac cttcgtagcg gcctttaatc
     4801 agcaggagac gatcgtcttc cacttccaga tggacagtca cttccggatt ggtcagcttc
     4861 acgcgcgcgg actcaatatg ctgatttaaa ccgccgccga cgtaacgttc cacatcaatc
     4921 gagctaaaat catgtttgcc acggcgcttc acgcgtacgc agaaggtttt gccttccagc
     4981 tgatcgcgat actgaaccaa cgctttctca aaaatatcgt gcatgtcggt aaacggcacg
     5041 tcttcgactt cgagaatatg gtggataccc ggaatacggg tcagggcgtc gcgaatagcc
     5101 agacgctggt tttcatcttt tgcgcgaact tcgatgttat cccagtggcg gacgacagcg
     5161 agcgtctcat catagtgctt taaaacgtta cgaatgttcc cggtaaggat ttttataaag
     5221 cgcaagcgca cagattggct tttgatggtg atttccggga acaatttaat gataaacttc
     5281 atggcggcaa tggttcgttg gcaagcctta agcgacttgt atagggaaaa atacagcagc
     5341 ccacacctgc ggctgcatcc aggcgcggaa gtataccact aacatcgctt tgctgcgcac
     5401 atcaccttac cattgcgcgt tatttgctat ttgccctgag tccgttacca tgacggggcg
     5461 aaaaatattg agagtcagac attcattatg ccgaagaaaa atgaggctcc cgccagcttt
     5521 gaaaaggcgc tgagcgagct ggaacagatt gtaacccgtc tggaaagtgg cgacctgccg
     5581 ctggaagagg cgctgaacga gttcgaacgc ggcgtgcagc tggcacgtca ggggcaggcc
     5641 aaattacaac aagccgaaca gcgcgtacaa attctgctgt ctgacaatga agacgcctct
     5701 ctaacccctt ttacaccgga caatgagtaa tggactttcc gcagcaactc gaagcctgcg
     5761 ttaagcaggc caaccaggcg ctgagccgtt ttatcgcccc actgcccttt cagaacactc
     5821 ccgtggtcga aaccatgcag tatggcgcat tattaggtgg taagcgtctg cgacccttcc
     5881 tggtttatgc caccggtcat atgtttggcg ttagcacaaa cacgctggac gcacccgctg
     5941 ccgccgtaga gtgtatccac gcttactcat taattcatga tgatttaccg gcgatggatg
     6001 atgacgatct gcgtcgcggt ttgccgacct gccatgtgaa gtttggcgaa gcaaacgcga
     6061 ttctcgctgg cgacgcttta caaacgctgg cgttctcgat tctaagcgat gccaatatgc
     6121 cggaagtgtc ggatcgcgac agaatttcga tgatttctga actggcgagc gccagcggta
     6181 ttgccggaat gtgcggtggt caggcattag atttagacgc ggaaggcaaa cacgtacctc
     6241 tggacgcgct tgagcgtatt catcgtcata aaaccggcgc attgattcgc gccgccgttc
     6301 gccttggtgc attaagcgcc ggagataaag ggcgtcgtgc tctgccagta ctcgacaagt
     6361 acgcagagag catcggcctt gccttccagg ttcaggatga catcctggat gtggtaggag
     6421 atactgcaac gttgggaaaa cgccagggtg ccgaccagca acttggtaaa agtacctacc
     6481 ctgcacttct gggtcttgag caagcccgga agaaagcccg ggatctgatc gacgatgccc
     6541 gtcagtcgct gaaacaactg gctgaacaat cactcgatac ctcggcactg gaagcgctag
     6601 cggactacat catccagcgt aataaataaa caataagtat taataggccc ctgatgagtt
     6661 ttgatattgc caaatacccg accctggcac tggtcgactc cacccaggag ttacgactgt
     6721 tgccgaaaga gagtttaccg aaactctgcg acgaactgcg ccgctattta ctcgacagcg
     6781 tgagccgttc cagcgggcac ttcgcctccg ggctgggcac ggtcgaactg accgtggcgc
     6841 tgcactatgt ctacaacacc ccgtttgacc aattgatttg ggatgtgggg catcaggctt
     6901 atccgcataa aattttgacc ggacgccgcg acaaaatcgg caccatccgt cagaaaggcg
     6961 gcctgcaccc gttcccgtgg cgcggcgaaa gcgaatatga cgtattaagc gtcgggcatt
     7021 catcaacctc catcagtgcc ggaattggta ttgcggttgc tgccgagaaa gaaggcaaaa
     7081 atcgccgtac cgtctgtgta atcggcgatg gcgcgattac cgcaggcatg gcgtttgaag
     7141 cgatgaatca cgcgggcgat atccgtcctg atatgctggt ggtcctcaac gacaacgaaa
     7201 tgtcgatttc cgaaaatgtc ggcgcgctca acaaccatct ggcgcagctg ctttccggta
     7261 agctttactc ttcactgcgc gaaggcggga aaaaagtttt ctctggcgtt ccgccaatta
     7321 aagagctgct caaacgtacc gaagaacata ttaaaggcat ggtagtgcct ggcacgttgt
     7381 ttgaagagct gggctttaac tacatcggcc cggttgacgg tcacgatgtg ctggggctta
     7441 tcaccacgct gaagaacatg cgcgacctga aaggcccgca gttcctgcat atcatgacca
     7501 aaaaaggtcg tggttatgaa ccggcagaaa aagaccccat cactttccac gccgtgccta
     7561 aatttgatcc ctccagcggt tgtttgccga aaagtagcgg cggtttgccg agctattcaa
     7621 aaatctttgg cgactggttg tgcgaaacgg cagcgaaaga caacaagctg atggcgatta
     7681 ctccggcgat gcgtgaaggt tccggcatgg tcgagttttc acgtaaattc ccggatcgtt
     7741 acttcgacgt ggcaatcgcc gagcaacacg cggtgacctt tgccgccggt ctggcgattg
     7801 gtgggtacaa acccattgtc gcgatttact ccactttcct gcaacgcgcc tatgatcagg
     7861 tgctgcatga cgtggcgatt caaaagcttc cggtcctgtt cgccatcgac cgcgcgggca
     7921 ttgttggtgc tgacggtcaa acccatcagg gcgcttttga cctctcttac ctgcgctgca
     7981 taccggaaat ggtcattatg accccgagcg atgaaaacga atgtcgccag atgctctata
     8041 ccggctatca ctataacgac ggcccgtccg cggtgcgcta cccgcgcggt aacgcggttg
     8101 gcgtggaact gacgccgctg gaaaaactgc caattggcaa aggcattgtg aagcgtcgtg
     8161 gcgagaaact ggcgatcctt aactttggta cgctgatgcc agacgcggcg aaagtcgctg
     8221 aatcgctgaa cgctacgctg gtcgatatgc gttttgtgaa accgcttgat gaagcgttaa
     8281 ttctggaaat ggccgccagc catgaagcgc tggtcaccgt agaagaaaac gccattatgg
     8341 gcggcgcagg cagcggcgtg aacgaagtgc taatggccca tcgtaaacca gtacccgtgc
     8401 tgaacattgg cctgcctgac ttctttattc cacaaggaac tcaggaagaa atgcgcgccg
     8461 aactcggcct cgatgccgcc ggtatggaag ccaaaatcaa ggcctggctg gcataatccc
     8521 tactccactc ctgctatgct taagaaatta ttcatagact ctaaataatt cgagttgcag
     8581 gaaggcggca aacgagtgaa gccccaggag cttacataag taagtgactg gggtgaacga
     8641 atgcagccgc agcacatgca acttgaagta tgacgagtat agcgggagtg gcagcatgca
     8701 atacaacccc ttaggaaaaa ccgaccttcg cgtttcccga ctttgcctcg gctgtatgac
     8761 ctttggcgag ccagatcgcg gtaatcacgc atggacactg ccggaagaga gcagccgtcc
     8821 catcattaaa cgcgcgctgg aaggcggcat aaatttcttt gataccgcca atagctattc
     8881 cgatggcagc agcgaagaga tcgttggtcg cgcactgcgg gatttcgccc gtcgtgaaga
     8941 cgtggtcgtt gcgacgaaag tgttccatcg cgttggtgat ttaccggaag gattatcccg
     9001 tgcacaaatt ttgcgctcta tcgacgacag cctgcgccgt ctcggcatgg attatgtcga
     9061 tatcctgcaa attcatcgct gggattacaa cacgccgatc gaagagacgc tggaagccct
     9121 gaacgacgtg gtaaaagccg ggaaagcgcg ttatatcggc gcgtcatcca tgcacgcttc
     9181 gcagtttgct caggccctga agctacaaaa acagcacggc tgggcgcagt ttgtcagtat
     9241 gcaggatcac tacaatctga tttatcgcga agaagagagc gagatgctgc cactgtgtta
     9301 tcaggagggc gtggcggtga ttccgtggag cccgctggcg cgggggcgac tgacgcgtcc
     9361 gtggggagaa actaccgcac gactggtgtc tgatgaggtg gggaaaaatc tctataaaga
     9421 aagcgatgaa aatgacgcgc agatcgcaga gcggttaaca ggcgtcagcg aagaactcgg
     9481 tgcaacacga gcacaagttg cgctggcctg gttgttgagt aaaccgggca ttgccgcacc
     9541 gattatcgga acttcgcgcg aagaacagct tgatgagcta ttgaacgcgg tggatatcac
     9601 tttgaagccg gaacagattg ccgaactgga aacgccgtat aaaccgcatg cggtcgtagg
     9661 atttaaataa gagtccaggc ctgatgagac gtgacaagcg tcacatcagg catcggtgca
     9721 caactacgac agaataccca gcggccagtg atgaccgata aagtacagga tgcctgcgga
     9781 aatcaccccg gcgacaatat cgtcgatcat gatccccatg ccgccatgta cattgcgatc
     9841 aaaccagcgg atcggccacg gcttccacat atccagaata cggaaaatca caaacccggc
     9901 ggcaacccac tgccagtcat tggtcggcag cgccatgagc gtgatccaca taccaataaa
     9961 ttcgtcccag acaatgctgc cgtgatcgtg cacgcccata tctttcgccg tttgatgaca
    10021 aagatagacg ccgatacaga tccccaacat caccaccagc gagtagagct gccagggtaa
    10081 aaaggtcatc agataccaga acggaatcgc tgccagcgat cccatcgtgc caggaacgat
    10141 tgggcttaat ccacttccga atccgacagc aagcagatgc cacggattac tcatcttcag
    10201 gcgacttttc gcgacatctt tatggcgtgg caaaatggtc atatcctttc cagtcaaacg
    10261 tgacaggttc gccgtcacga ataaaacaaa gcccttcgat atcggcggtc atttgcccga
    10321 tacaggtaaa cggtacgccc aggtgtccga gagccacatc cagcgcgcca cggttcagtt
    10381 ccggcacagt gaaacacaac tcgtaatctt caccgccaga gagcgcccag cgcagcgcct
    10441 gttccggttc aacatggcga gaaagcgcat cagaaaacgg cagcaatgcc aggtcaatac
    10501 gtgcgccgca gtcgctggct ttcacgatat gcccgagatc ggaaatcaga ccgtcagaga
    10561 gatcgatagc tgaatttgcc agatcgcgca gtgcctgccc ctgtaaaata cgcggcgatg
    10621 gacggagatg acgtttgatc aagtagtcag catctttagc atcggcaacc tgcaaacggt
    10681 tttgcaaaat cgccagcccg gcggcgctat cgcccggtgt accggtcaca tagatccagt
    10741 cacccggttt cgccccagag cgcgttaagg ctcgtcccat cggaacaaag ccgtggatac
    10801 ccaacgtcat tgataatggc ccacgcgtgg tatcgccgcc aatgagttgc atatcgtaat
    10861 aattgagaag atcaaacaaa ctgtcgctga aggactcaag ccacgcttcg tctacgtccg
    10921 gtaaggttaa tgccagcgtc agccaggccg gatcggcccc catcgctgcc agatcgctta
    10981 ggttcactgc cagtgcttta tacgccagat cagcaggatc gatatcatgg aggaagtggt
    11041 tgcccgccac cagcgtatca gtgctgatcg ccagggtctg tttctcgggg atattgagaa
    11101 gagcgcaatc gtcgccgatg cccagttcga catcaagacg agaacttctt acacggtcaa
    11161 aataacgggc aatcagggag aactcgccac atgccatacg ttatgcctca gcaggtaaaa
    11221 aagaaaaggc cggcgatcgc ggaacggtct tccgtgaatc taccggcctg gatatcactt
    11281 tttgttaggg cgaatcacag gtgctgcttt atcgagtacg ccgttgacga acttatggct
    11341 gtcttctgcg ccgaacgatt tcgccagttc gatcgcttcg ttaatggcca ctttgtatgg
    11401 cacatcgcta cgtttagaca gctcgtacag cgcaatgcgc agtactgctt tttctacctg
    11461 tcccagttct tccagcaggc gggacaggta tggcttcatc agtccgtcga ggtatgcggt
    11521 gttagtcgcc accccggcca gcagctcacg gaagtacagg acgtcaacgt cttttacatc
    11581 ctgttcagcc aggaactggt attcaacatc agcgatgtcg ttctgggaca actgccagga
    11641 gtagagcgcc tggacggcac actcacgagc gcggcgacga gcagcaggtt tcacggattt
    11701 ccccttacta atttcaggcc ttgatggctt tcaatacatt aatcatttca agcgcggtca
    11761 gtgcagcttc tgcacctttg ttgccagctt tggtgccagc acgttcgatc gcttgttcaa
    11821 tgctttcagt ggtcagaacc ccaaaagcaa ccggaatttc gctgtcctgg gcaacatgcg
    11881 ccaggccgtt gcttgcacca ccagcgacat attcaaagtg ggcagtgcca ccacgaataa
    11941 ccgtacccag cgcaatcacc gcgtcgtatt taccggtttt agccagtgca cccgccgcca
    12001 gcggcagctc ataggcacca ggcacccaaa caacggtaat gttttcatct tttacctgac
    12061 cgatacgttt cagtgcgtca attgcacctt ccagcaggct gtcattgata aagttgttga
    12121 aacgcgcgat ggtgatggcg acgcgagcgt ccggggtagc aacgttagct tcaataatgt
    12181 tcattttctt ccttcgggtt cgagtatggc cccgcagggg ggcggatttt agcataatat
    12241 ttcgtgcgct gcttcccttt cgagccggga gatcatgcac ccactaaatg caggcaaaca
    12301 tccgggccta catgacgtat ctctttgaat ttaaattggg gggcgtcggc taatttctca
    12361 agccctggca gcgtgcataa tccgcgggcg tcgctgccta atagtttagg tgcgatatag
    12421 acaatcagct catcgactaa acccgcctgc agcaatgcgc cagcgagcgt tggccccgct
    12481 tccacccaga tactgttaat ttgctgttta cccagttgca tcatcagtac aaccagatcc
    12541 agatgacctt tatgctctgg aatcagcaag gtacgcaccg tttccggcca ctcactagaa
    12601 tcttcctgcg tacgcgcgaa ccaggtttcg ccgggctgct gcacaatgcg atgttccggc
    12661 gtcacgcgat tttggctatc aatcacaata cgtactggct ggcggagatt ttgttgcggg
    12721 tagagcgcct gagtttgttc atccagttca gaccaacgca ctgttaaggc tggatcatcc
    12781 gccagcaccg tggcgctgct agttaaaatg gcatgacttt gtgcgcgctg tcgttgtacg
    12841 tcgcgccgcg cctgaggcga agtgatccac tggctttcgc cgctcgccat cgccgtgcgg
    12901 ccatcaagcg atgcgccaag ttttaactga atataaggaa agccggtgcg catccgcttg
    12961 agaaagcctt tattcaattg ctcggcttca ctcatcatca gcccgtggct gacgtcaatg
    13021 ccagcctgtt gcagacggta aagtccacgc ccagcgacct gcgggttagg atcttgcatc
    13081 gaggcaacca cgcgcgctac gccagcggcg atgagtgcgt cacagcacgg tggcgtacga
    13141 ccatgatggc tacagggttc gagtgtgaca taggcggtcg cacctttggc tttttcaccc
    13201 gccatacgca acgcgtgtac ttcggcatgt ggttcacccg cacgttggtg gtaaccttca
    13261 ccgacaattt cgccatcttt gacaatgacg cacccgacat tcgggttggg atgcgtggta
    13321 aaacgtcctc gttgcgccag ctttagcgct cgcgccatgt aatactcgtc ctgcacggct
    13381 tagtcctcca ggcgcgcgat ctcttcgcca aattctttga tatcttcgaa actgcggtag
    13441 acagaggcaa aacggatata ggcgacttta tcgagctttt tcaattgctc catcaccaga
    13501 ttgccaatca tcttgctcgg cacttcgcgc tcaccggtgg cgcgcagctg cgatttaata
    13561 tgattgattg ccatttcgac gtcatcggaa ctcaccggac gtttttccag cgcccgcagc
    13621 attccgctac gcaatttctc ttcattaaac ggttcacgca cgtcgttgct ttttacaaca
    13681 cgcggcataa ccagctccgc cacttcaaag gtggtgaaac gttcattaca caccagacac
    13741 tgccgacggc ggcgtacgga tgaaccctcg cccacgagac gagagtcaat taccttagtg
    13801 tccacggcga aacagaatgg gcaatgcata cggcgtcctg accaggtggt taacagaaat
    13861 ctattttacc ctgaactgac gatacaacaa aggcgcaacg cttttgagat aaaggatcga
    13921 tgctttcgct gcttttaccc tctaccatta ggctaattgc gacgagaaag ggacgaccta
    13981 tgaatactaa cgtttttcga ctgctcctgc tgggaagcct gttcagcctt agcgcctgtg
    14041 tgcagcagag cgaagtgcga cagatgaaac acagcgtcag cacgctgaac caagagatga
    14101 cgcaactcaa taaagaaacg gtcaaaatca ctcagcaaaa caggctgaat gcaaaatcca
    14161 gcagtggggt ttaccttctg ccaggagcga aaacaccggc aagactggaa agccagatcg
    14221 gtactttacg tatgtcgctg gtgaatatta cgcctgatgc agatggcacc accctgacgc
    14281 tacgtattca gggcgagtcg aatgatccct tgcccgcatt cagcggaact gttgaatatg
    14341 ggcaaattca gggaacaata gacaactttc aggaaatcaa tgtgcagaat caattgatta
    14401 atgcccctgc cagcgtcctc gcccccagcg atgttgatat tccgttacag ttaaagggta
    14461 tttctgtaga acagttagac ttcgtacgta tccacgatat tcaacctgtt atgcagtaaa
    14521 cgtatttcgg ggcgattttg tgcgtctcgc aacatctttc cccgtcattt tgttactccg
    14581 cttacatcac cctgattgat agtaaaagtt tgcaacaagg gtgaaagtca gtacaatccc
    14641 tgcccgaatg tgtgtaaacg tgaacgcaat cgattacgta aatgatagaa ctgtgaaacg
    14701 aaacatattt ttgtgagcaa tgatttttat aataggctcc tctgtatacg aaatatttag
    14761 aaacgcaatt tgcgcctttt tcactcccgc aagggatttt caaacagtgg catacatatg
    14821 aaaaaaacat tactggcagc cggtgcggta ctggcgctct cttcgtcttt tactgtcaac
    14881 gcagctgaaa acgacaaacc gcagtatctt tccgactggt ggcaccagag cgttaacgtt
    14941 gtcggaagct atcacacccg tttcggaccg cagatccgca acgataccta ccttgagtac
    15001 gaagcattcg ctaaaaaaga ctggttcgac ttctatggtt atgcggatgc gccggtattc
    15061 ttcggcggta actccgatgc taaaggtatc tggaaccacg gttctccgct gtttatggaa
    15121 atcgaaccac gtttctccat cgacaagctg accaatactg accttagctt cggtccgttc
    15181 aaagagtggt acttcgcgaa caactacatt tacgacatgg gtcgtaataa agatggtcgc
    15241 cagagcacct ggtacatggg tctgggtacc gacatcgata ctggtctgcc gatgagcctg
    15301 tccatgaacg tctatgcgaa ataccagtgg cagaactatg gcgcagcgaa cgaaaacgag
    15361 tgggacggtt accgtttcaa aattaaatac tttgtgccga ttaccgatct gtggggcggt
    15421 cagctgagct acatcggctt caccaacttc gactggggtt ccgatttagg ggatgacagc
    15481 ggtaacgcaa tcaacggtat taagacccgt actaataact ctatcgcttc cagccatatt
    15541 ctggctctga actacgatca ctggcactac tctgtcgtag ctcgttactg gcacgacggt
    15601 ggtcagtgga acgacgatgc agaactgaac ttcggcaacg gcaacttcaa cgttcgctct
    15661 accggctggg gtggttacct ggttgtaggt tacaacttct gattatgaaa atgccgggat
    15721 ttattcccgg catttcagat tgttaacaac gtgcgctttg ttcatgccgg atgcggcgta
    15781 aacgctttgt ccgacccaca aaagcacgta aattcaatat attgctgaaa taacgtagtc
    15841 ctgataagcg tagcgcatca ggcaatttta cgttttaatc acttcttctt gttcatcatc
    15901 gctttcaggt cagcgaatgg gttgtacttc gcttcaccga catctttctg cgcatcttcc
    15961 cctgcgataa cggtcgtacc atactgatcc gcttcggtat atttcgaatg ctcatgatcg
    16021 tggcaataga gacaaagcaa ttcccagtta ctgccatctt ccgggttatt ggtatggtcg
    16081 tgatcaatgt ggtgaacagt aagttcacgc aggttggaat aaacaaactc gcgggaacag
    16141 cgaccgcaga cccacggata tatttttaat gctttttcgc gatagccgct ttctaaccgc
    16201 gcatagtttt ttgggatgat agccatgaag agttacctgg atgaaatgta gggttacgcg
    16261 cagtttccca agatgcgggg attttcgcaa tctccataca tcaaaaaaaa tcccgatctt
    16321 ctgaccggga ttttcaacat caacgggaac ttgattacgg cagaattgac ggctgatccg
    16381 ccccttcttt ttccactttc tgctgcaaca tgtgttcgcg cttcataccc agtttcagag
    16441 ccaacgcaga tgccacatag atggaagatg cagtaccgat ggaaacaccg ataagcatgg
    16501 tcagcgagaa gccttccagt accggaccac cgaagaggta cagcatcagg ataaccatca
    16561 aggtagtacc ggatgtgatc aaggtacggt gcagcgtctg ggtcaaggac acgttaaaga
    16621 tttcgtaagg cgtaccgcga cggatcttgc ggaagttttc acgaatacgg tccgatacca
    16681 cgatactgtc gttaagcgag taaccgataa ccgacatcaa cgatgccaca atggtcaggt
    16741 caatctcgat atggaataac gacaaaatac ccagcgtaat gataacgtcg tgcgccagcg
    16801 caataaccac ccctgccgcc agtcgccact caaagcgaaa acctacgtac acaaggatag
    16861 acagcagcgc cgccatcaac gccatcgcac cggtttgcgc aaggtctgcc cccacgctcg
    16921 gaccgacgaa ctcaatacgc ttcactgctg cattctgatt ggtggattcg ttaatcacct
    16981 tcagaacctg gctgcccagc acctgaccgc cggtttcgcc ttcagcaggc ggcatacgga
    17041 ccatgatgtc gtggctgcta ccaaagtttt gcagcattgg ctcttcaaaa ccggcttttt
    17101 gcaatgcatc acgcattacg tcaatttcag ccggtttttc gagcgtaatt tcgataaccg
    17161 taccaccggt gaaatccagc ccccagttaa agccgcgcac gcccataata acgatagcgg
    17221 cgattaacag cagaccagag atgccgaaag cccagtagtc ccagcgcata aagtcataga
    17281 ctttacggcc gtggtttagt tgttcaacag tatattcctg tgccacatcg cactcctcag
    17341 attgacagct tcttgacgcg cttgccgcca tatagcaggt ttacgatggc acgcgtaccg
    17401 acaatcgcgg taaacatcga cgtcgccaca ccgataccgg tagtaatcgc gaacccttta
    17461 attgccccgg tacccactgc gtacaggatg atgactttaa tcagcgtggt gatgttcgca
    17521 tcgaagatag aactgaatgc gccacgataa ccttcatcaa ttgcctgttg aacagtacgt
    17581 ccgttgctca actcttcttt aatacgttcg ttgatcagta cgttcgcatc gaccgccacc
    17641 gcaagggtta agacgatacc cgcgatacct ggcatactca gcgttgcgcc tggcaacagc
    17701 gacataatgc cgacgattaa gatcaagttg gcaatcagag cactggtcgc aatcagacca
    17761 aacttcttat agaagatgat catgaacaga atagaaacca gcagaccggc aaggcaagcc
    17821 tccagcccct gttcaatgtt ctgcataccc agggttgggc caatggtacg ttcttcaaca
    17881 atctgaatcg gcgcgatcaa cgcaccggca cgcagcagca gtgacagctg acgggcttcg
    17941 ttcgggttgt tgatgccggt gatacggaag ctgttaccca gacgagactg gatgttggcg
    18001 atgttaatca cctcttcctg tttcaccaga accgcacgac catttgcatc tttcttaccg
    18061 ctgtctttgt actccacaaa cagggttgcc atcggtttgc cgatgttgtc cttagtgaag
    18121 ttagacatga tgttaccacc agcgctatcg agcgagatgt taacctgcgg ttggttgtat
    18181 tcgtcctggc tggaagtgga gtcggtgata tggtcaccgg tcagaattac gcgtttgtac
    18241 agcacaactg gctgaccttc gcgggtctgt ttcacttcag agtcgcccgg tacgcgaccg
    18301 gatgccgcag cggcctggtc aacgttggtg tttaccagac ggaattccag cgttgccgtc
    18361 gcacccagaa tctctttcgc acgcgcagtg tcctgaatac ctggcagttc aacaacgata
    18421 cggtcagcac cctgacgctg aaccaccggc tccgccacgc caagttggtt tacacggtta
    18481 cgcaggatat tgatgttctg ctgcaccgca tattcacgcg cttcactcag acgagcatcg
    18541 ctcattaccg cacgcagctg gttgctgccc tggctactaa tcaccaggtc cggatggcgc
    18601 ttgctcagat acgcaatggc ttcatcacga gctttagcat cgcggaaagt aatgctcagg
    18661 ccgtagttgt tttctttacg aacggtggta tacgggatgc ccttttcacg cagatcgctg
    18721 cgcaggctat cgatattttg ttcctggagt ttgccaagcg cggtgtccat atccacttcc
    18781 atcaggaagt gaacgccgcc acgcaggtca aggccgagct tcatcggctc agcgtgaata
    18841 gctgccagcc agcgcggcgt tgccggggca aggttaagcg ccacgacgta tttgtcaccc
    18901 ataacgccca ttaatgcttc acgagcgcgc agctgggtgt cagtggagtc gaagcgcgca
    18961 agaatagcgc cctcttccag tgccacagac ttagcagtta ttttttcttc ttgtaacgtt
    19021 ttctggacct ggatcagcgt ttgctcactg gcggcgactc cgcgcgcacc agtgatctga
    19081 acagccggat cctcaccaaa caggttggga agcgcataca gcagaccgat gacaatcacc
    19141 acgatcagca tgacgtactt ccacaaagga taacggttta acacggcaat tcccttaggg
    19201 aaaaatttta attacagcgc cttcatggtg cctttcggca ggacggcagc tacgaagtca
    19261 cgtttaataa ctacttcagt ggtgtcattc agcgcgatag caatgtagcc gttttccgct
    19321 actttggtta cgcgaccaac caggccaccg ttcgtcagaa cttcatcacc tttggcaatg
    19381 gagtccatca gctttttgtg ttctttggtg cgcttctgct gtggacgcag gatcatgaaa
    19441 tagaaaatca gaccgaatac caccagcatc aaaatcaaag acatcgggct accttgcgcc
    19501 ggtgcacccg ttgccgctac cgcatcagaa ataaaaaagc tcattaaatt tccctcatta
    19561 ttaatattaa tcaacgttca aaggtggtac ttctcgcccc tgacgctggt aaaaatcagt
    19621 tacgaagctc tctaatttac cctcttcaat agccttgcgt aaacccgcca tcaaacgctg
    19681 gtagtaacga aggttatgaa tggtgttgag tcgcgcgcct aatatttcgt tgcaacggtc
    19741 aagatgatgc aagtaagcgc gtgaataatt gcgacaggtg tagcaatcac actcaggatc
    19801 gagtgggcca gtatcgctct tatacttcgc attgcggatt ttcaccacgc catcggtcac
    19861 gaacaaatga ccatttcggg cgttgcgggt tggcattacg cagtcaaaca tatcgatacc
    19921 acgacgtacg ccttcaacca ggtcttctgg tttaccaacg cccatcaggt aacgcggttt
    19981 gtctgccgga atttgcgggc atacatgctc cagaatgcgg tgcatatctg ctttcggctc
    20041 acccacagcc agaccgccga cagcgtagcc atcaaaaccg atatctacca gacctttaac
    20101 agaaatatca cgtaaatctt cgtaaacgct gccctgaatg ataccaaaca gcgcattttt
    20161 gtttccgaga ctgtcaaaac gctcacggct acgcttcgcc caacgcagag acatctccat
    20221 ggagcgtttt gcgtaatccc agtcagcagg atacggcgta cactcatcaa agatcatgac
    20281 gatatccgaa ccaagatcgt actgaatctc cattgatttt tcaggatcga ggaaaatcgg
    20341 atcgccgttg atcgggttac ggaagtgcac gccctgttcg gtgattttac gaatatcgcc
    20401 aaggctgaag acctggaagc cgccggagtc ggtgaggatc ggccccttcc actgcataaa
    20461 atcgtgcaga tcgccgtgca gtttcatgat ttcctggccc gggcgcagcc acaggtggaa
    20521 ggtgttgccg aggataattt gcgcgccagt ggcttcaact tcttccggcg tcatcccttt
    20581 tacggtgccg taggtgccaa caggcataaa acaaggcgtt tccactacgc cacaatcaaa
    20641 gaccaggcgg ccacggcgtg cgcgaccgtc ggtggtgtcc agttcaaatt tcattttttc
    20701 tccactacgt cagaaaaaca gtccaacgtt ttaaaccagc gccgcggaat tactccccga
    20761 cgcgctcatt aattgcctgc ggattgtacg tgataaacat cgcatcaccg taactaaaaa
    20821 agcgatattt ctcttctacc gctgctttat aggcgttcat ggtgtgttga taaccggcaa
    20881 aggccgaaac cagcataatc agcgtcgatt ccggcaggtg gaagtttgtc accagcgcat
    20941 cgaccacttt gtactggaag cccggataga taaagatttg ggtatcgtca aagaacggtt
    21001 cgataagatc gtttttcgcc gcctgagccg cgctttccag cgaacgtact gaggtggtgc
    21061 caaccgcaat cacccggtta ccgcgcgctt tcgccgccag taccgcgtct accacatcct
    21121 gcggcacttc agcgtattcc gagtgcatga tgtgatcttc aatggtgtcg acgcgcaccg
    21181 gctggaaggt gcccgcacca acgtgcaacg tcacaaacgc catctccacg cctttggcgc
    21241 gtaatttttc cagcaaaggt tcgtcaaaat gcagacctgc ggtcggggct gcaaccgcgc
    21301 ccggtttttc gctataaacg gtttgataaa gttcgcggtc agcgtcttcg tccggacggt
    21361 cgatatacgg cggcagcggc atatggccga tgctgttgag aatatccagc accgagcgtt
    21421 catcattaaa ttcgacttca aacagtgcgc cgtggcgcgc ggtcattgtt gcgttaatac
    21481 tttcgtcatc gcccagcagc agttctgcgc caggttttgg cgctttcgag gcgcgaatat
    21541 gcgcaagaat gcgtttgtcg tcgagcatcc gttcaaccag cacttcaatc ttgccgccgc
    21601 tggctttacg cccaaacagg cgcgccggga tcacgcgggt attattaaaa accagaagat
    21661 cgccggggtt gagcttatca agtaaatcgg tgaaagtacc gtgcgtcagc gcgcccgtcg
    21721 gcccgtccag cgacagtaaa cgacagctac tgcgttcagg catgggatag tgggcaatca
    21781 gggattcggg caattcaaag gagaaatcgg taacgcgcat gacactgact cagactaaaa
    21841 taagaggcgg gtagtctagt gccggggcgc tctccctgca acctttaccc ccttttggat
    21901 agaatataat cgatgaattt tttagctcac ctgcatttag cccatctcgc ggaaagctcg
    21961 ctttccggca atttactggc tgatttcgta cgcggaaatc ctgaagaaag ttttccgccc
    22021 gacgtcgtgg ctggcattca tatgcatcga cgtatcgacg tattaaccga caatctgccg
    22081 gaagtccgcg aagcacggga atggtttcgt agtgaaactc gccgcgttgc gcctattacg
    22141 ctggatgtca tgtgggatca ctttctttcc cgccactggt cgcagctgtc gccggacttt
    22201 ccgctacagg aatttgtctg ttatgcccgc gagcaagtga tgacgatttt gccggactca
    22261 ccgccacgtt ttatcaatct gaacaattac ttgtggtcag agcagtggct ggtgcgctat
    22321 cgcgatatgg atttcatcca gaacgtgtta aacggcatgg caagccgccg cccacgtctg
    22381 gatgccctgc gtgactcctg gtacgattta gacgctcatt atgacgccct cgaaacccgc
    22441 ttctggcagt tttatccgcg gatgatggcg caggcgtcac acaaggcgtt ataaaacgtt
    22501 agttcatcca taccgtagcc gaaatggcag gcaaagccag aatcccgtca gtcagttgcc
    22561 catgcccttc tttgcattgc cattgcacgg cattgagcaa cggtgacgcg ggtagcacca
    22621 cttcacaagc ttcgccacgg ttgattgcca ccagtacacg ctgctgattc agcacgcgga
    22681 caaataccac cacgttatct tccgcataca gcacctgaca gccgccatga cgtagcgcct
    22741 gacttttctt acgcagcgca atcattcgct ggtacagcgc gaataacgcc gtatcctgct
    22801 tttccacctg ccaggggaac ggtttacggc aaaacggatc gtttttgcca tccagtccta
    22861 cttcatcacc gtaataaatg cacggtacac caggccaggt gaacagccag accaccgcca
    22921 gcggcaggcg cgcaatatcc cgaccgagca gcgttttaaa tcgcgcagta tcgtggctgt
    22981 cgagctgatt aaacatacgt aattgttgtt gatgagaaag ccctgcgcgg taattatcca
    23041 tccaggccat acaggtttgg gcatcaattt gctgcggatc gtaagagata tcggtattgg
    23101 caagaaatcc ccacaacggg aatgtgaagc cacgatagtt catggcggca tcttccacat
    23161 cggcctgtaa ccattgccgt gcatcgccaa aatgttcgcc gacaatatac gcttccggct
    23221 gggtttcttt cgccgcttcg gtgatcccag caacgtgctg cata
//
LOCUS       DAZGJW010000067        22964 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZGJW010000067 DAZGJW010000000
VERSION     DAZGJW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.067"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..190)
                     /locus_tag="ACHKIK_002671"
                     /pseudo
     CDS             complement(1..190)
                     /locus_tag="ACHKIK_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(296..1225)
                     /locus_tag="ACHKIK_002672"
     CDS             complement(296..1225)
                     /locus_tag="ACHKIK_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014538499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083821.1"
                     /translation="MVVIWTSIPLAISSVILWPKACKYFKMKAWRIVVEIRKYYSLSP
                     INYFILYVGALSFMVAVLSQFLFGNKFFILTGFSIVAMSVSFSIDLYNRTIYILKKVW
                     SGMLGKILISLMATLSYIITNYLSRHWLYVTTDLEPRFFSEFINIVSIFLTPLSCLII
                     LGFLSLFIFIPEFIGITFLFLFTPIRENFLRNRLKPLTELSVRMRTGKRIERLTPSEL
                     VLLNSKVMFFRTFSSPVFVCLIFYLFSWVSSITGGNMDTIARAGLVSYYYHSSKIDDV
                     SRMKYYNYDNEKFSTAIFNGEKLEFLLLMTNNI"
     gene            complement(1537..1868)
                     /locus_tag="ACHKIK_002673"
                     /pseudo
     CDS             complement(1537..1868)
                     /locus_tag="ACHKIK_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2546..3523
                     /locus_tag="ACHKIK_002674"
     CDS             2546..3523
                     /locus_tag="ACHKIK_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO9083822.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PVSLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLQPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3802..4542)
                     /locus_tag="ACHKIK_002675"
     CDS             complement(3802..4542)
                     /locus_tag="ACHKIK_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9083823.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGGDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(4663..4773)
                     /locus_tag="ACHKIK_002676"
                     /pseudo
     CDS             complement(4663..4773)
                     /locus_tag="ACHKIK_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
     gene            4963..5544
                     /gene="bfpA"
                     /locus_tag="ACHKIK_002677"
     CDS             4963..5544
                     /gene="bfpA"
                     /locus_tag="ACHKIK_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bundle-forming pilus major subunit BfpA"
                     /protein_id="HFO9083824.1"
                     /translation="MVSKIMNKKYEKGLSLIESAMVLALAATVTAGVMFYYQSASDSN
                     KSQNAISEVMSATSAINGLYIGQTSYSGLDSTILLNTSAIPDNYKDTTNRKITNPFGG
                     ELNVGPANNNTAFGYYLTLTGLDKAACVSLATLNLGTSAKGYGVNISSENNTTSFGNS
                     ADQAAKSTAITPAEAATACKKPDSTNKVTYFMK"
     gene            5591..5992
                     /gene="bfpG"
                     /locus_tag="ACHKIK_002678"
     CDS             5591..5992
                     /gene="bfpG"
                     /locus_tag="ACHKIK_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bundle-forming pilus protein BfpG"
                     /protein_id="HFO9083825.1"
                     /translation="MRTVILFFCLLVSCFTQAQESANKSEKLFSGDTTSVALQASERT
                     IKNAKTDWSVIKGQTLYGVLSDWAQQSGWTVLWHANYNYTLNASAVFKDMSLDDAVKK
                     LLESMGNVSPRVFIKFYNGNHVILVTQSPEG"
     gene            5999..7657
                     /gene="bfpB"
                     /locus_tag="ACHKIK_002679"
     CDS             5999..7657
                     /gene="bfpB"
                     /locus_tag="ACHKIK_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bundle-forming pilus secretin BfpB"
                     /protein_id="HFO9083826.1"
                     /translation="MKLGRYSLFLLCPLLASCSGNGFYKDNLGVIDKNILHADTSLLK
                     SKNKEHYKSSDMVSKTDSIYIGNSSFQTYHGEPLPGKLEGVHGIILRSSTPLGFDEVL
                     SMIQDSSGIPIVKHTTKDVISGGVSSKSLAATVAEKMNSATGGKSTDQFDHLLLEVSS
                     EHQLMDVNYQGALSTFLDKVAANYNLYWTYESGRIVFSNEETKRFSISILPGGKYTSK
                     NSISSDSNSSSGNSGSSGSSSSDSGAELKFDSDVDFWKDIENSIKLILGSDGSYSIST
                     STSSVIVRTSSANMKKINEYINTLNAQLERQVTIDVAIYNVTTTDSSDLAMSLEALLK
                     HNGGVLGSVSTSNFAATSGTPSFTGYLNGNGDSSNQVLLNLLAEKGKVSVVTSASVTT
                     MSGQPVPLKVGNDRTYVSEIGTVLSQSSTSTTASTSTVTSGFLMNLLPQVADDGNILL
                     QYGVTLSELVGSNNGFDQATVNGTVIQLPNVDSTTFVQSSMLRNGNTLVLAGYEKKRN
                     ESVDQGVGTTSFKLLGGALNGSASRTVTVICITPRIIDLKASGE"
     gene            7654..8862
                     /gene="bfpC"
                     /locus_tag="ACHKIK_002680"
     CDS             7654..8862
                     /gene="bfpC"
                     /locus_tag="ACHKIK_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bundle-forming pilus protein BfpC"
                     /protein_id="HFO9083827.1"
                     /translation="MIKNNLGVAVIGSKQYAVNLLWGSSQDTETTNQALNKSLTLMSS
                     KLYSVIGRFQGEQFAVGDKNIGHKRGQVTLLSAIDFDGSSFCGLFPADNELWLVIGVD
                     KDGMVHFDKSFHSKDDAKKFFFDHVAYGYPWDRTYSPSDVGVGESRSISELSLIKGKK
                     LKEKGAGQLMPLLLSGVGIIIFFVIIYNFMNLWLSGRDKDILNEEPSPAVSEEVRNIP
                     WEGKSKPGSLFSKCIKEMDSFRFQAASVPGWMPENKVTCNDSDIRFSVYRSGGLNIWY
                     ENPGLFFHSGDIPDITRNDNDKITFTWPLETVRYDSSILDVHTLNKTQEIIRYLSRQF
                     ENSWIEIKFEPSVPDGNTERVGFSFSFNNDPRILLPILSEVNGLIITNIDYDFSGSNW
                     NVKGVFWGRA"
     gene            8840..9334
                     /gene="bfpU"
                     /locus_tag="ACHKIK_002681"
     CDS             8840..9334
                     /gene="bfpU"
                     /locus_tag="ACHKIK_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bundle-forming pilus protein BfpU"
                     /protein_id="HFO9083828.1"
                     /translation="MFFGGELSLKKILFTFLFFVSSVVNAEVNTSVDVSGKNDSRKEE
                     NKLPDVDLEKYKDELLSYQELQRKNMMLKLQSENQKLESEIKKSGSFISYDTNENTIY
                     LMSIMTTPKKGLCATIFNGAISRVCKGDIINEHSVVSDITGNYVEIRSINDISDPVQK
                     IFLR"
     gene            9341..10948
                     /gene="bfpD"
                     /locus_tag="ACHKIK_002682"
     CDS             9341..10948
                     /gene="bfpD"
                     /locus_tag="ACHKIK_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bundle-forming pilus extension ATPase"
                     /protein_id="HFO9083829.1"
                     /translation="MMLNKTEKTSDLMFERFKRNVSEIVTGDGGELELTVEQRKYFLI
                     FKNGDFLVSSCHMKHHLVQMLREIATRKGYPNLTIYEVNLKDIRLLYEASLKTVQNNG
                     QDLLPVEKRASMLLFECAEMRVSDLHIKVYDAEADIYIRKDGDMELLRQIESNTAHSI
                     LASLYNNADDSDATYKINAYQAARIVASKSRLALPPVIQAVRLQFNPLGQGGRYLIAR
                     FLYTDKSEKQKEMDPTRFGFHHSHAESFSRMRNLPIGINIISGPTGSGKSTTLKNLLE
                     LLYIEKKKKVNIISIEDPPEYEIDGTAQLPITNVETEAQRGEEYRKAITAALRSDPDI
                     IMPGEARDAEVINLLFTAAMTGHQVWTSLHANNALAIFDRLKDQGVDEFKLTDPELIT
                     GLVAQRLVRRLCDQCSITLTEYIASGGEISDTDRKIISGHETSVRFPNPRAKKCCRDG
                     YNGRTILAEVIEPDSKLLRLVAEGKREDALHYWLTSLHGMALKEHAWLKIISGEICVM
                     DAVNKISGIDNITEERKKYLFSRDNEI"
     gene            10955..12013
                     /gene="bfpE"
                     /locus_tag="ACHKIK_002683"
     CDS             10955..12013
                     /gene="bfpE"
                     /locus_tag="ACHKIK_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bundle-forming pilus protein BfpE"
                     /protein_id="HFO9083830.1"
                     /translation="MKEKLNRLLFTSKTRMRVFSKLSKYLSNGVPVTFALAELYKFTS
                     DEGRKKDNPDAFALQRWLISVRNGKTLAEAMRGWVPFDELSIISAGEISGNVHQALDD
                     IIYMNDTKKKVKGALAGIIYPVVLLLTTCLYLHIFGTQVVPAFSGILPVEKWQGAGRT
                     MYYLAVFVQDYLVITLLSFMMVILLILATLSRWTGRLRLFFDRFIPWSIYKTIIGCGF
                     LLSLASLINAGIPVPEALRIIMKTASPWYKERLVAIRYRLLNGERNLGEALNSSGYIF
                     PSKQMIMDLRSYAALGGFDEMLNKLSVQWQDDSVAYITKQMTVLKNVAIIIMGLVFMW
                     IVSGMFSLQQQISDAARF"
     gene            12035..12991
                     /gene="bfpF"
                     /locus_tag="ACHKIK_002684"
     CDS             12035..12991
                     /gene="bfpF"
                     /locus_tag="ACHKIK_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bundle-forming pilus retraction ATPase"
                     /protein_id="HFO9083831.1"
                     /translation="MNIINELNFADLLISKDSYNFRFLEGQPYPICNVPDNYNAEVQE
                     MVQELECIREAKGNEFFYLHLGVPYRVAVVQTISGSGFFLRKLKLPVPSLDSLGFSEP
                     FLNTLKLMGKKRGLLLVSGATGSGKSTTIYALLTYYVQKYGDIAISIEDPPEVPVQGN
                     YGDNQEGVWFQMDARELGGYENAMISAMRYNPRYIFLGEIRSAKTAKEAIRAAVNGHL
                     VVSTIHGSSVQGAIYALQQIASAEGEADLVRSIIADGLLGIVHQELRFTENGQRSLSA
                     NILCNDGSASISSKIRSGKLELLSSEIEQQRIMLSHGKNLIS"
     gene            12998..13747
                     /gene="bfpP"
                     /locus_tag="ACHKIK_002685"
     CDS             12998..13747
                     /gene="bfpP"
                     /locus_tag="ACHKIK_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bundle-forming pilus pre-pilin peptidase BfpP"
                     /protein_id="HFO9083832.1"
                     /translation="MQESIFLLLVFIYAATITSFIWLAVERLPHQLKWVDNPVSDITI
                     WSPGSKCNNCGKKIRWYYLIPVLGYFLCRGECGYCHAKVPVRYPLTEFICGVCCVIIF
                     VFLGGRLYDAVIVSLLFLCLVFLALIDLRENWLPACVTYPLFWAGMITPGFASSDDKI
                     FGAFAGFLIMYISMKLVSALRKEDVFAGGDIALATAAGAWLGIDKMPFFLILSSFIFI
                     LYSLPARLRGQVFVPMGPALSASFFICLVYH"
     gene            13729..14175
                     /gene="bfpH"
                     /locus_tag="ACHKIK_002686"
     CDS             13729..14175
                     /gene="bfpH"
                     /locus_tag="ACHKIK_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bundle-forming pilus lytic transglycosylase
                     BfpH"
                     /protein_id="HFO9083833.1"
                     /translation="MFSLSLITGVAACVLNASSVNNIEPALLLSVMTVEGGKPGSVSI
                     NKNGSHDLGIMQINTHTWLKLISKSFFNGDERSAYTKLKDDACFNISVGAWILRRSII
                     FEGGNLWEGVGRYHSATPEYKYRYIDKVKNVYGQITYSQSIHQRNM"
     gene            14196..14741
                     /gene="bfpI"
                     /locus_tag="ACHKIK_002687"
     CDS             14196..14741
                     /gene="bfpI"
                     /locus_tag="ACHKIK_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bundle-forming pilus minor pilin BfpI"
                     /protein_id="HFO9083834.1"
                     /translation="MFILRNKRKESGLSLIEASLVIILSGLVIVLVQSAISKIIKYAV
                     HENALHDVSYVMSYIQDTLQTSCYGKENIVCDGVISSVKSNINSGNKIKVDVKKDGKY
                     VSVYILYTLSGNEYFGRNEDFGSMLFTHVSAYSGRMDSSTGVVSSVLGWWKVNSDDTI
                     FLGGDNNKNLFFYNNFRVDVL"
     gene            14738..15289
                     /gene="bfpJ"
                     /locus_tag="ACHKIK_002688"
     CDS             14738..15289
                     /gene="bfpJ"
                     /locus_tag="ACHKIK_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bundle-forming pilus minor pilin BfpJ"
                     /protein_id="HFO9083835.1"
                     /translation="MIRKESGLSLIEVMIGVLFFSIVSLSVYNYYNRSAEQHYMNNVS
                     VVMCRYADALSYYLQDSTATNGNGSVSLSTLVASGYLPPMQETSGVGEYEIKMYVDKN
                     KNGLIALYGGEYYSLTSENIKKFLGIRGASAKDNKIISAGGYYSLPVSDFTGFRTVNL
                     RGLMLIPSIQTSGQNCDRGGYDF"
     gene            15279..15767
                     /gene="bfpK"
                     /locus_tag="ACHKIK_002689"
     CDS             15279..15767
                     /gene="bfpK"
                     /locus_tag="ACHKIK_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bundle-forming pilus minor pilin BfpK"
                     /protein_id="HFO9083836.1"
                     /translation="MIFEGRQQKGLSLIEIAMVIILSVVFISGGSYIISLFKVFAQSC
                     VVATDMNSVRNIIISSDVEYNTGKYDASSITNDNRFNKYQVYFGNDGYTLISGNSPVS
                     VDVFFITCYLAKYNCVWILNTNNKIYAKHMSAVLPLELLSAYGLNYSGESGVTGLLFK
                     RY"
     gene            15770..16219
                     /gene="bfpL"
                     /locus_tag="ACHKIK_002690"
     CDS             15770..16219
                     /gene="bfpL"
                     /locus_tag="ACHKIK_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bundle-forming pilus assembly protein BfpL"
                     /protein_id="HFO9083837.1"
                     /translation="MLFFWCGFFSLIVSILNISMGYNDRLFLDEQNRIIKENIYVDYL
                     TVNAAVLSEICTTDADKCKDKTSILSDGLSESLKDKLSGYDNSITPEFCINHGKVIVY
                     TTYHFNISRYKDSSLFNLSDVPNSDRLACSINDNSGIAIIKTFNKKG"
     gene            16847..17254
                     /gene="tnpA"
                     /locus_tag="ACHKIK_002691"
     CDS             16847..17254
                     /gene="tnpA"
                     /locus_tag="ACHKIK_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFO9083838.1"
                     /translation="MVFAPKYRRQVFYREKRRAIGSILRKLCEWKSVRILEAECCADH
                     IHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRNREFWCRGYYVDTVGKNT
                     AKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
     gene            17364..18716
                     /locus_tag="ACHKIK_002692"
     CDS             17364..18716
                     /locus_tag="ACHKIK_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO9083839.1"
                     /translation="MPLLNDLLDFSDHPLMPPPSAQLFAEHLPVEWIQHCLTLSAHAT
                     VRRRRLPEDMVIWMVVTMAFFRNEPITDVVRRLNLSADGESGINLLARSAVTQARQRV
                     GAAPEEWLFRQTAQTWGSERYLKDDWHGLQLFAIDGAQFRTPDEPELREYYGSANTST
                     ERQSAYPVMRLVALMNLGSHILLNAVTAPYRRSETVLAHSMLATIPDNSITLFDKLFY
                     SADLLLTLNQQGCNRHWLLPAWKNIAAETEESYGSGDRLLKLKVSLQARKKNPALPEF
                     WYARAVTYEVNGVEKTGLTSLPADRYKAKEVAELYHSRWEIEVGFRNLKSSLLNNALV
                     LRSRKVELLEQEVWGMLLAYNLIRREATKAAEKHKKAASEISFKFAFQFIATEMIVLG
                     NTASPGTIPKRLEHLRGALEVVFITKRPRPSRPRAVKISKTRYPVKRSTAPLKRTALR
                     "
     gene            18715..19450
                     /locus_tag="ACHKIK_002693"
                     /pseudo
     CDS             18715..19450
                     /locus_tag="ACHKIK_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            19537..20179
                     /gene="tnpA"
                     /locus_tag="ACHKIK_002694"
                     /pseudo
     CDS             19537..20179
                     /gene="tnpA"
                     /locus_tag="ACHKIK_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
     gene            20179..20526
                     /gene="tnpB"
                     /locus_tag="ACHKIK_002695"
     CDS             20179..20526
                     /gene="tnpB"
                     /locus_tag="ACHKIK_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9083840.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERTGIRI"
     gene            20546..22118
                     /locus_tag="ACHKIK_002696"
                     /pseudo
     CDS             20546..22118
                     /locus_tag="ACHKIK_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            22394..>22964
                     /gene="perA"
                     /locus_tag="ACHKIK_002697"
     CDS             22394..>22964
                     /gene="perA"
                     /locus_tag="ACHKIK_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BFP system transcriptional regulator PerA"
                     /protein_id="HFO9083841.1"
                     /translation="MLTSKKEMQSSKNKQEENLTLLLTNYISYQNIVIFTGGNQFKIR
                     NKKEFTEYTIEPNSLFFLAKNTHWDMEVVGIDNSNPYRKIIIDDALIKLLHSISSDDS
                     CYVKKNIFTAKLNKMQLNIVFNIIADIKHSGNNKKIFKILYLLSFFNDHNDIVNVILS
                     ASSKSIVDRVIKVIELDIAKNWKLGDVSSS"
BASE COUNT         6730 a         4597 c         5293 g         6344 t
ORIGIN      
        1 acgtcctccc cagcagcaac gatagtaact attgcttctt ccacctgaac agcaggatgg
       61 actgaatgcc gtgcaagcga gcgacgagag aaacattcat gcccggtttc atcgtctgca
      121 ggataatggc gatcttctcc cgagaattct tacgtttacg agcgtcttgc cctaaccggc
      181 tcccgatcat ctcaaattgg cgttagtttc acctgataat gctgcatgcg aagtcttttt
      241 cggaagacag aaaatgaaat gtatgcaaga tttttcagca ccgaatgtgc tccgttcata
      301 tattatttgt cattaaaagt aaaaactcca atttctctcc attaaatatt gctgtggaaa
      361 acttctcatt atcgtagtta taatatttca ttctggaaac atcatcaatc ttagaagagt
      421 gataataata actgacaagt cctgctcttg cgattgtatc catatttcct cctgtgatac
      481 tgctaaccca cgaaaacaaa taaaaaataa ggcaaacaaa gacaggagat gaaaatgttc
      541 taaaaaacat aacttttgag tttaacaata ccaactcaga tggtgtaagt ctctcaattc
      601 tctttcctgt tctcattctt acagatagtt cagtaagggg ttttaatctg tttctcagga
      661 aattttctct gataggggta aacaagaaca aaaatgttat tcctataaac tcaggtataa
      721 aaataaacaa cgaaaggaaa cctaatataa ttagacagga cagaggcgta agaaaaatgc
      781 ttactatatt aataaactca ctaaaaaacc ttggttcaag atcagttgtg acataaagcc
      841 aatgtcgtga gaggtaattt gtaataatat atgataaagt ggccattagt gatattaaaa
      901 tctttcccaa catcccagac catacttttt ttaaaatata aatagtacgg ttatacaaat
      961 caatcgaaaa agaaacactc attgcaacaa tggaaaatcc agtcagaata aaaaatttat
     1021 tgccgaataa gaattgactt agtacagcga ccataaatga caaagctcca acatacaaaa
     1081 taaaataatt aattggagaa agactataat attttcttat ttctacaaca attcgccatg
     1141 ctttcatttt aaaatattta caagcttttg gccataagat gacagatgat atcgctaatg
     1201 gaattgatgt ccatataaca accaatatta taaatgctgg ttgtattaga gctataaaag
     1261 tcgcactctt aaaaaaagtt ggttaaagaa cgttaaaaga agacataata aaattaattt
     1321 aaagaagatt ctgctaagca ttttatttag ttgatataac acagtagaaa tctcgcttta
     1381 taaaaacatt acgaacacta atataattat aatccttaac tgataatttt cgcagctacc
     1441 tataacaaga tacctatagt aatttaattg aatagctccg taaacaatgg atattgttcc
     1501 cgtacatcca cgtatactgg attggatact atatttctat acatttttgt cacttagccc
     1561 attactggtt ccgtaaccac cgatatccca gtgtactatg aaaatatact tcgttgtggt
     1621 gttcgaagat atctgcaaag ttctttaccg ttttcacgaa actctctatc attatgtagt
     1681 tttcctggct acgtaccgct tagtttagac ttcagtctat caattctgac gaactgatgc
     1741 atcggataag aacgataggc tgaactgttt tcagtcatca actcaacgag gaatgtcggc
     1801 agattgctgc caaagcgacg ctacgctaca ccttgcgtaa cgttctgtac tttttcgcag
     1861 ccatatccat cggtattacc tgtaacaaca gttgctgaac taccagtgct atcttatcaa
     1921 tccctggcat tgaaacacac atatgaagtg tacttcggat atgaccgtac gctcaggaag
     1981 tacgacagat tccgtcaaaa attacctgta aagacagaca tcagaaaaaa cagtgctcgc
     2041 cgcagaccgg tgaccgggcg aagcccgcga accggcgagg aagcgcaatg tatctgtagt
     2101 tacagtgagc aggtacgccc tgaagccctt tcataacacg gttgtgtgcg ctgtccttct
     2161 ggaatgattt atccacatat ccggaggccc gatccagtaa actagatcca tgaatgatca
     2221 acaaaggatc cattaaagat ccccatatca ctgcaaacct tgtcactcat gggccgggac
     2281 catattcaca taagctgtgg catgttatgg ataaactgta acaggttcat gataagctgt
     2341 attcagtaat gcatatactg aagtaggtga atgacataaa ctatggtcag tacgccagac
     2401 tcagctgtta aatacaggct gcagattttt cttcagtcag ttagcggggt tctaacatcc
     2461 gattttctgt ttattctttt actgtccaca ggcaggaggc tttctggaaa acgaaaattc
     2521 agacatcaaa aaactgttcg gcgaggtgga taagtcgtcc ggtgagctgg tgacactgac
     2581 accaaacaat aacaacaccg tacaacctgt ggcgctgatg cgtctgggcg tctttgtacc
     2641 gacccttaaa tcactgaaga acagtaaaaa aaatacactg tcacgtactg atgccacgga
     2701 agagctgaca cgtctttccc tggcccgtgc tgagggattc gataaagttg agatcaccgg
     2761 cccccgcctg gatatggata acgatttcaa gacctgggtg gggatcattc attcctttgc
     2821 ccgccataac gtgattggtg acaaagttga actgcctttt gttgagtttg caaaactgtg
     2881 tggtatacct tcaagccagt catcccgcag gctgcgtgag cgcatcagcc cttccctgaa
     2941 gcgcattgcc ggtaccgtga tctcgttttc ccgtaccgat gagaagcaca cccgggaata
     3001 catcacccat ctggtacagt cagcctacta cgatactgag cgggatattg ttcagttaca
     3061 ggccgatccc cgcctgtttg aactgtacca gtttgacaga aaagtccttc tccagcttaa
     3121 ggcgattaat gccctgaagc gacgggagtc cgcccaggca ctctacacct ttatagagag
     3181 cctgccccgg gatccggcac cggtatcgct ggcgcggctg cgtgcacgcc tcaatctgaa
     3241 gtctcctgta ttttcccaga accagacggt cagacgggca atggagcagc tgcgcgagat
     3301 tggatatctt gattacacgg agatccagcg ggggcggaca aaactcttct gcattcacta
     3361 ccggcgtccc cggttaaaag cgccgaatga tgagagtaag gaaaatccgt tgcaaccttc
     3421 acctgcggaa aaagtcagtc cggagatggc ggagaagctt gccctgcttg agaaactggg
     3481 catcacgctg gatgacctgg aaaaactctt caaatcccgc tgaacataaa ctgtagtcag
     3541 tgaagagtgc tcctttactg actacagctt atattatcag gtgcagtgag tggtctgctc
     3601 actgcagttt atattcagtt tcctgcagtg ctgcctgtgg ctgagctgcc atctgcctgt
     3661 cccttacgtg agtcaccccg taacctgatg ctgaggcatt gctcccttta taaaacatgg
     3721 tttactcact acagcttatc tacctgctcc agcttatgtt atgtctgttc tgctgaccac
     3781 tgcttatata aggaagtgcc gttacttcag gggaggcagt gtacgcagga tctccgcagc
     3841 atcccgaccg tcacctgtga aaggcactgc cagcgtggca gccatatcca gtgcaaacac
     3901 tctggtataa acctccatcg aacgtggatc cctgtgacca gccagtgcct ggatgacttt
     3961 ccggggctgg cggtgataga gcatgtgcat gatatagctg tgccggaacg tgtgtggtgt
     4021 gaccggaatc gaaaagtgta ccccgtcagc ttcggcccgt ctgacagcct gcttcagcca
     4081 gttgcgcatg gtttcgtcgg tcacggccca taatggttca cgacgacggg gccgggtggt
     4141 gatcatccag ctttccatct gcctgacata gcttatatct gtcagcggaa ccaggcggac
     4201 ttcatctttt ggcgggcgtc cgcgtcgcgc acgcactttt tcggacagga tccgcacaaa
     4261 cggtcttaca ccatccagat caaatgattc cggcgtcagc atccgggctt cgccaatacg
     4321 cattccggta ttccagaggg tggcgaacag catatggtga cgctgatccg gcatatagaa
     4381 gagaagggca ctcacttccg gggccagcag gtaggccggt gtggcccccg tggaggtggc
     4441 cattcttctc agggagagcg ctgttgcaaa atcccccccc ggcgcaatgg gtaacaggga
     4501 gacgcgttct ggtgaattct gcaggggaat gacattgttc atgagttatt tttcacgtag
     4561 cacacgcact gtttatggat acaggtattc tgacctgaac gcccggatcc ttccacgaca
     4621 ataaatcgca gcgcgcggat ccttttgttt gattatcacg cattattgcg ggtaaaacca
     4681 aatacgtaaa ggcgcatttt gccgctggga tcccgtctta agccagtcat ggtgcgggct
     4741 gagggctggt ttaccgtttt tgggagaaaa tggggttatg ccaggagtac cggaagtcaa
     4801 attcatgggg atattgactg tttatgtact gggggggacg gaaatatata aaaaaaaaag
     4861 aaaaaaagat tatttttttt cttggtgctt gcgtgtcttt tttagtttta agattattcc
     4921 gtgacctatt aatacggggg ttttataagg aaaacatttt ttatggtttc taaaatcatg
     4981 aataagaaat acgaaaaagg tctgtctttg attgaatctg caatggtgct tgcgcttgct
     5041 gccaccgtta ccgcaggtgt gatgttttac taccagtctg cgtctgattc caataagtcg
     5101 cagaatgcta tttcagaagt aatgagcgca acgtctgcaa ttaatggtct gtatattggg
     5161 cagaccagtt atagtggatt ggactcaacg attttactta acacatctgc aattccggat
     5221 aattacaaag atacaacaaa cagaaaaata accaacccat ttggggggga attaaatgta
     5281 ggtccagcaa acaataacac cgcatttggt tactatctga cgcttaccgg gctggataaa
     5341 gcggcatgtg ttagtcttgc aaccttgaac ttaggtactt cagcgaaagg ctacggtgtt
     5401 aatatctcta gcgaaaataa cactacatca tttggtaata gcgctgatca ggctgctaaa
     5461 tcgactgcta ttactcctgc tgaagcggca actgcatgta aaaaacctga ttcaaccaat
     5521 aaagttacat attttatgaa gtaatctaca taatacgccc tgttttgggc gtattatatg
     5581 ggaggtatat gtgaggacag taatcctttt tttttgtctt ttggtatcct gttttactca
     5641 ggcacaggaa agtgcaaata aaagcgagaa acttttttca ggggacacaa cgtctgttgc
     5701 actgcaggca agtgaaagaa caattaaaaa tgccaaaact gactggtctg taataaaagg
     5761 acagacttta tatggcgttc tttccgactg ggcgcaacag agcggatgga ctgtattatg
     5821 gcatgcaaat tataactata ctttaaatgc atctgcagtg tttaaggata tgtcgctgga
     5881 tgatgcagta aagaaactac tagaatcgat gggaaatgtg tcacccaggg tctttattaa
     5941 attttataac gggaatcatg tcattctagt tacacaatca cccgaaggat aatttcaaat
     6001 gaaacttggc aggtattcac ttttcttatt gtgtccgctc ctggcatctt gctcgggtaa
     6061 tggattttat aaagataatc tgggtgtaat cgataaaaat attctgcatg ctgacacctc
     6121 attgctgaag tcgaagaata aagaacatta taaaagcagt gatatggtaa gcaaaacaga
     6181 cagtatttat ataggaaata gcagttttca gacatatcat ggagaacccc tgcccggtaa
     6241 attagaaggt gtacatggaa ttattctgag atcaagtaca cctctgggat ttgatgaagt
     6301 actctctatg attcaggatt ccagtggtat tcccattgta aaacatacca cgaaagatgt
     6361 gatcagtgga ggggtgtcca gcaaaagtct ggccgcaact gtcgcggaaa aaatgaacag
     6421 tgcaacaggc ggaaaaagta ctgatcaatt tgatcatcta ctgcttgagg tatcatcaga
     6481 acatcagctt atggatgtta attatcaggg cgcgctttcc acgtttcttg ataaagttgc
     6541 ggcaaactac aacctgtact ggacatatga gtctggcagg attgtttttt caaacgagga
     6601 aactaaacgc ttcagtataa gtatattacc gggtggtaaa tatacatcaa aaaactctat
     6661 cagttcggac agcaatagca gttcgggcaa ttcgggcagt tcgggcagta gttccagtga
     6721 ttctggagca gagttgaaat tcgattcaga tgtggatttc tggaaagata ttgagaactc
     6781 gataaaactg atactgggca gcgatggttc gtattcaata tcgacaagta catcgtctgt
     6841 tattgtcaga acatcttctg caaacatgaa gaagataaac gaatatatta atacactgaa
     6901 tgcacagctt gaaagacagg ttaccattga tgtcgcgatc tataatgtta caacaactga
     6961 cagcagtgat ctggcaatgt ctctggaagc tttgctaaag cataacggag gtgttctggg
     7021 ctctgtttca acctcaaatt ttgcggcaac aagcggcacc ccttcattta caggatatct
     7081 gaatgggaac ggcgactcaa gcaaccaggt tttgcttaac ttgttagccg aaaaaggaaa
     7141 agttagtgtg gtgacatctg catctgtaac tacgatgtca ggtcagccgg ttcctttgaa
     7201 agtaggaaat gacagaacat atgtatctga gatagggact gtattaagcc agtcatcaac
     7261 cagtacaaca gccagtacgt caactgttac cagcggtttt ctgatgaacc tgttaccgca
     7321 ggttgctgat gacgggaata tattgttgca gtatggtgta acgctttcag aacttgttgg
     7381 ctcaaataat ggtttcgatc aggcaactgt taatggaacc gtaattcaat tacctaatgt
     7441 tgattccaca acctttgtgc aatcaagcat gcttcgcaat ggtaatactc tggttctggc
     7501 aggatatgag aagaagagaa acgaaagtgt ggatcagggc gttgggacaa ccagttttaa
     7561 actcttgggt ggcgcactga atggaagtgc ttcccgaaca gtcactgtca tttgtataac
     7621 cccgagaatt attgatctca aggcttctgg cgaatgataa agaataatct tggcgtagcg
     7681 gtaattggtt cgaaacaata tgccgtgaat ttattatggg gaagttctca ggatacagaa
     7741 acaacaaatc aggctctgaa taagagcctg acgttgatgt cctcaaagtt atattccgtt
     7801 attggacgat ttcagggtga acagtttgcc gtaggagata aaaatattgg ccacaaaaga
     7861 ggccaggtga ccttactgtc tgcaattgat ttcgatggca gttctttttg tgggctgttt
     7921 cctgctgata atgaattatg gcttgtcatc ggtgttgata aagatggaat ggttcacttt
     7981 gacaagtcct ttcacagtaa ggatgatgca aaaaaattct ttttcgacca tgtagcatat
     8041 ggatacccgt gggacagaac atacagtcct tcagatgttg gagtcggtga aagcaggagt
     8101 atttctgagc tttcactaat caaagggaag aaattaaagg aaaaaggcgc cggtcaacta
     8161 atgccattgc tgttatctgg cgttggcatt ataatattct ttgtcatcat atataatttt
     8221 atgaacttat ggttaagtgg cagagataag gatattctta acgaagaacc atctccggcc
     8281 gtcagtgagg aagtcagaaa tatcccgtgg gaagggaaaa gcaaaccggg ctcattgttt
     8341 tctaaatgta taaaagaaat ggattcattc cggttccagg cagcatccgt tccaggctgg
     8401 atgcctgaaa ataaagtaac ctgtaatgac tctgatataa gatttagtgt ttatcgttct
     8461 ggcggactta atatatggta tgaaaatccg ggcctgtttt ttcacagtgg agacatccca
     8521 gacattacca gaaatgataa tgataaaata acatttacat ggcctcttga aacggtcagg
     8581 tatgacagtt cgatactcga cgtgcatacc ttgaataaga ctcaagaaat aataaggtat
     8641 ctttcacgtc agtttgagaa tagctggata gagataaaat ttgaaccgtc cgtgccggac
     8701 ggcaatacag aacgagtggg tttttctttt tcatttaaca atgatccgag aatcttactc
     8761 ccaattcttt ctgaagtaaa tggactgatt atcactaata tcgattatga tttttcagga
     8821 agtaactgga acgtgaaagg tgttttttgg gggagagctt agtttgaaaa aaatattgtt
     8881 tacttttttg tttttcgttt cttcagtcgt gaatgctgag gtcaatacaa gtgttgatgt
     8941 ttccggtaag aatgactcta gaaaagaaga aaataaattg cctgatgttg atttagaaaa
     9001 gtataaagat gaattgcttt cctatcagga actccagcgt aagaatatga tgcttaaatt
     9061 acagtctgaa aatcagaaac tggagagtga aataaaaaaa agtggatcat ttatctcata
     9121 tgatacaaat gaaaacacga tatacttaat gtctataatg acaactccta aaaaaggatt
     9181 atgtgcaacc atatttaatg gggctataag cagagtctgt aagggcgata tcattaacga
     9241 acattctgtg gtgagtgaca ttaccggtaa ttatgtggag atcaggagta ttaatgacat
     9301 atccgatccc gtgcagaaaa tttttctgag ataggtaagt ttgatgctaa acaaaacaga
     9361 aaaaacttca gatctgatgt ttgaacgatt taagcgcaat gtatctgaaa tcgtcacggg
     9421 agatggggga gagcttgagc ttactgttga acagagaaaa tactttctga tattcaaaaa
     9481 tggtgatttt cttgtttctt catgtcacat gaagcatcat ctggtacaga tgttacgtga
     9541 aatcgcaaca agaaaagggt atccaaacct cactatttat gaggtgaact taaaagatat
     9601 caggttattg tatgaagcat ctctcaaaac cgtacagaat aatggacagg atctattacc
     9661 agtcgaaaaa agagcttcaa tgctactttt cgaatgcgcc gagatgagag taagtgattt
     9721 acacatcaaa gtgtatgatg ctgaggctga tatttatata cgtaaagatg gggatatgga
     9781 actcttgcgt cagattgagt ctaatacggc gcattctatt cttgcatcat tatataataa
     9841 tgcagatgat tctgatgcaa cctataaaat taatgcttat caggctgctc gcatagttgc
     9901 ttcaaaatcg aggctggcgt tacctcccgt aattcaggcg gtgcgccttc agtttaaccc
     9961 tcttggtcaa ggtgggcgat atttaatcgc acgattcctg tatacagaca aaagtgaaaa
    10021 acaaaaagaa atggatccaa ccagattcgg attccatcat tcacatgctg aatcattttc
    10081 aagaatgcgg aatctcccaa ttggtataaa tattatctcc ggaccgacgg gttcgggtaa
    10141 atcaacaacc ctgaaaaatt tacttgagtt attgtatatc gagaaaaaga aaaaagttaa
    10201 tattatcagc attgaagatc cacctgagta tgaaattgac ggcacggctc agttacctat
    10261 aacgaacgtt gaaacagagg cacaaagggg tgaagaatat cgtaaagcca ttacagccgc
    10321 tttgcgttct gatccggata taataatgcc aggggaagcc agggatgctg aagtgataaa
    10381 ccttcttttc actgcagcaa tgacaggaca tcaggtatgg acatcattac atgcaaataa
    10441 tgcactggct atattcgatc gattgaaaga tcagggcgtg gatgaattta aacttacaga
    10501 tccggaattg atcactggat tggttgccca gcgattagtc agaagattgt gcgatcaatg
    10561 tagcataaca ttaacagaat atatagcaag tggcggggag atatctgaca cggacagaaa
    10621 aataatctca ggtcatgaaa catctgtacg atttcccaat cctcgtgcta aaaaatgttg
    10681 tcgggatggg tataatggaa ggacaatcct cgcggaggtt attgaaccgg actcaaaatt
    10741 actaaggttg gttgctgaag gtaagcgaga ggatgctctg cattactggt taacatctct
    10801 tcatggtatg gccttaaaag agcatgcatg gttaaaaata atttctggtg aaatatgcgt
    10861 tatggatgca gtaaataaaa tttcaggcat cgataatatt acagaagaaa ggaaaaaata
    10921 tcttttcagc agagataacg agatatagtt ttctatgaaa gagaaattaa acaggctgct
    10981 attcacgtcg aaaacgagaa tgcgtgtttt tagcaaatta tccaagtatc taagtaatgg
    11041 tgttccagta acgtttgccc tagctgagct ttataagttt acgtctgatg agggaagaaa
    11101 aaaagataat ccggatgcat ttgccctgca aagatggtta atttctgtaa gaaacgggaa
    11161 aacattggct gaagcgatga gggggtgggt accttttgac gaactgtcaa taatttccgc
    11221 cggggaaatc tcaggtaatg ttcaccaggc attggatgat atcatttata tgaatgatac
    11281 aaaaaagaaa gtaaaaggcg cactggcagg gattatttat ccagtagtcc tgcttctgac
    11341 gacatgtctg tatttgcata tatttggaac tcaggttgtt ccggcatttt caggcatcct
    11401 gcctgtagag aaatggcagg gcgcaggcag gactatgtat tatcttgctg tattcgttca
    11461 ggattatctt gtcattacac tgctgtcttt tatgatggtg atattattaa tactggcaac
    11521 tctgtcaaga tggaccggaa ggttgcgtct cttctttgac agatttattc cctggtcgat
    11581 atacaaaacc attataggat gtggtttctt gttatcactg gcatcgctta ttaatgcagg
    11641 tatccctgta ccggaagcct tacgaataat aatgaaaacg gcaagtccgt ggtataagga
    11701 aagattagtt gctataagat acaggttatt aaatggagag agaaatcttg gggaggccct
    11761 taattcatct ggttacattt tcccatccaa acagatgata atggatctcc gttcttatgc
    11821 tgcacttgga ggctttgatg aaatgcttaa taaactttca gttcaatggc aggatgacag
    11881 tgttgcttat ataacgaaac aaatgactgt tctgaaaaat gttgctatca ttattatggg
    11941 gcttgtattt atgtggatag tatcaggaat gttttcatta caacaacaaa ttagtgatgc
    12001 agcaagattc tgattttttc ttcggtgtaa tataatgaat attattaatg agctcaactt
    12061 tgcagattta ctgatctcaa aggattcata taatttccgt tttctagaag ggcaaccata
    12121 tccaatatgt aatgtgccgg ataattataa tgcagaagtg caggagatgg tgcaagaact
    12181 tgagtgtatc cgggaagcaa agggaaatga atttttttat cttcatcttg gggtacctta
    12241 tcgagttgcg gttgtgcaaa caatcagtgg ttcaggtttt ttcttacgta aactgaaact
    12301 gccggtacca tcactcgata gcctcggatt ttccgaacca ttcctgaata cattaaaatt
    12361 gatggggaag aaaagaggtt tgcttttagt tagtggagct acaggctctg gcaagtccac
    12421 aacaatctat gctttgctga catattatgt tcagaaatac ggtgatattg ctatttcaat
    12481 tgaagatcca ccggaagttc ctgttcaggg aaattatggt gataatcagg aaggcgtctg
    12541 gtttcagatg gatgcacgtg agcttggggg atatgaaaat gcaatgattt cagcgatgcg
    12601 ttataatcca agatatattt tcctgggtga gattcgtagc gctaaaacag cgaaagaggc
    12661 cattcgggct gcagtcaatg ggcatcttgt agtttcaaca atacacggaa gctctgttca
    12721 gggggctatt tatgccttac agcagatagc ttctgctgag ggagaagcag atcttgttcg
    12781 cagcatcatt gctgacgggc tgttaggtat agttcatcag gagttacgtt ttacagaaaa
    12841 cggacagcgg tcactcagtg caaacatttt atgcaacgat ggctctgcat ctatttcttc
    12901 aaaaataaga agcggtaaac tggagctttt gagtagtgaa attgagcaac aacgaattat
    12961 gttgagccat gggaaaaacc tgattagcta aaatattatg caagaaagta tatttctatt
    13021 attggttttt atatatgcgg ccacaattac cagttttata tggctcgctg tggaacgtct
    13081 gccccatcag ttaaaatggg tagacaatcc tgtttctgat ataacaatat ggtctccggg
    13141 aagtaaatgt aataattgtg gtaaaaaaat tcgttggtat tatcttattc ctgttttggg
    13201 gtattttttg tgtcgggggg aatgtggcta ttgtcatgct aaagttcccg tccggtatcc
    13261 acttacagag tttatctgtg gtgtttgctg tgttattata tttgtatttt tgggaggcag
    13321 actgtatgat gcagtaatag tttcgctact ttttctttgc cttgtttttc ttgctctgat
    13381 tgatttaaga gagaactggc taccagcttg tgttacgtac cctctatttt gggcaggtat
    13441 gattactcca gggtttgcat ccagcgatga taagattttc ggtgcatttg ccggtttttt
    13501 aataatgtat atttcaatga aactagttag cgccttaaga aaggaggatg tttttgcagg
    13561 cggagacatt gctctggcta cagcggccgg tgcatggtta ggaatagata aaatgccttt
    13621 ttttcttatt ttgtcttcat ttatatttat tctttactct cttccagcaa ggttaagagg
    13681 tcaggttttt gttcctatgg gaccagctct ttccgcgtca ttttttatat gtttagttta
    13741 tcattaatta caggtgttgc agcatgtgtt ctcaatgcca gttctgtgaa taatattgag
    13801 cctgcactat tactgtcagt tatgaccgtc gaaggtggta aacctggtag tgtatccatt
    13861 aataagaatg gaagccatga tttaggtatt atgcagataa atacccatac atggttaaaa
    13921 cttatcagta agagtttttt taacggagat gagaggtctg cttataccaa acttaaggat
    13981 gatgcatgtt ttaacatatc agtaggggcc tggatcctcc gcagatctat tatattcgag
    14041 ggtggtaatt tatgggaagg tgtgggccga tatcattcgg ccacaccaga gtataaatat
    14101 agatatatag ataaagtgaa gaatgtttat gggcaaataa catacagcca atcaatacat
    14161 caaaggaata tgtaagtaaa aatcatggtt cgtctatgtt tatattgaga aataaaagaa
    14221 aagagtcagg tttgtcactg attgaagcgt cattagtgat tattttatct ggcctggtta
    14281 ttgtgcttgt tcagtcagca atttcaaaaa ttattaagta tgctgttcat gaaaatgcac
    14341 tgcatgatgt ttcgtatgta atgtcttaca ttcaggatac attacagaca tcatgctatg
    14401 gaaaagaaaa tattgtttgt gatggagtga taagttcagt aaaatcaaac attaactctg
    14461 gtaataaaat aaaggttgat gttaaaaaag acggaaaata tgtatctgtg tatattttat
    14521 acactctgtc agggaacgaa tattttggca gaaatgaaga tttcggtagc atgttattta
    14581 cgcatgtaag cgcttattct gggagaatgg atagcagtac aggagttgta agttcggttc
    14641 tgggatggtg gaaagtaaat tctgatgaca caattttttt aggtggggat aataataaaa
    14701 atcttttctt ttacaataat ttccgggtgg atgttttatg ataagaaaag aaagcggcct
    14761 ttcattaatt gaagtaatga ttggcgtcct ttttttttcg attgtttctc tttctgtata
    14821 taactattac aacaggagcg cagagcaaca ttatatgaat aatgtctctg tcgttatgtg
    14881 cagatatgct gatgctctta gctactattt acaggatagc acagcaacca atggtaacgg
    14941 tagtgtatca ttatctactc tggttgcttc tggatatttg ccaccaatgc aagaaacgtc
    15001 tggtgttgga gaatatgaaa taaaaatgta tgtcgataag aataagaatg gtctgatagc
    15061 attatatggt ggagagtatt attcacttac ctctgagaac ataaaaaaat ttctggggat
    15121 tcgtggtgca tctgctaaag ataataaaat catatctgca ggtgggtatt attcattgcc
    15181 tgtatctgac tttacaggtt ttcgcactgt taatttaaga ggattaatgc ttattccttc
    15241 cattcagaca tcagggcaaa attgtgacag aggtggatat gatttttgaa ggacgtcagc
    15301 aaaagggact gtcgcttatt gaaatagcaa tggtaattat tttgtctgtg gtatttattt
    15361 ctggtgggag ttatattata tctcttttca aggtttttgc tcaatcttgt gttgtagcaa
    15421 cagatatgaa ttctgtcaga aatataatca tcagttcgga tgttgaatat aacactggaa
    15481 aatatgatgc atcatccata acaaatgaca atcgatttaa taaatatcag gtgtattttg
    15541 gaaatgatgg ttatacttta atatcaggaa attctcctgt tagtgtggat gtgtttttta
    15601 ttacctgcta tttggcaaaa tataactgtg tatggattct caataccaac aataaaatat
    15661 acgcgaaaca tatgtctgct gttctcccgt tagagttatt aagtgcatat ggccttaatt
    15721 attcaggaga gagtggtgtc acagggcttc tgtttaaacg gtactagtta tgttattctt
    15781 ctggtgtggt tttttttcat taattgtatc cattttaaat atttccatgg gttataatga
    15841 tcgcctattc ctcgatgaac aaaaccgaat tattaaagaa aatatatatg tggattatct
    15901 tacagttaat gctgctgtct tgtctgaaat ttgcacaaca gatgcagaca agtgcaagga
    15961 taaaacgagt attctgtcgg acgggctttc agaatcactg aaagataaat tgtctggata
    16021 tgataatagc attacacctg aattttgtat taaccatggg aaggttatag tctatacaac
    16081 ttatcatttt aatattagcc gttataaaga cagctcattg ttcaaccttt cagacgtgcc
    16141 gaatagcgac agattagctt gttcaataaa tgataattct ggtattgcta ttatcaaaac
    16201 attcaacaaa aagggatagc accaccagaa ctgtaatttt tttaggctat gtcccataat
    16261 tgtttcatca attaaaaatc atgccgccat gacagcaaat agtggtggaa acccatgaag
    16321 tcacttcaaa aaaatgaaca ggcctcatgg gttttattaa cataaaaacc acaacttccc
    16381 gccttcattg cgctcccgac gacaatatta atagctagaa aatgtttaga acaatgattc
    16441 aatttcctaa ccatcaagtg attaagtgtg tgattcatag acatcgtttc actataatac
    16501 ctcactatga acagggaaat tcagcagcgg ctacagtggg taaaactcta tgaaacgtcc
    16561 ggtgatgcgc aatgccgatc agttaaggat cggttgaccg ctccctaaac tgcggcacta
    16621 taacggcttc cacaccaggg agccgttttc ttgtgcacag aaaaccccca gcctagctgg
    16681 gggttccgga aagctttcag ctttgagcct gttattaaaa ccccttttga tttgttaaaa
    16741 caccttgcgg tctggcaact gcaagtgtca aacaagaaat caaaaggggg tcccaatggg
    16801 gaactaaaag agcttagcgc acacccgatg gaactgtaaa tatcacatag tatttgcgcc
    16861 aaaataccga agacaggtgt tctacagaga gaagcgtaga gcaataggca gtattttgag
    16921 aaagctgtgt gagtggaaaa gtgtacggat tctggaagct gaatgctgtg cagatcatat
    16981 ccatatgctt gtggagatcc cgcccaaaat gagcgtatcc ggctttatgg gatatctgaa
    17041 agggaaaagc agtctgatgc tttacgagca gtttggtgat ttgaaattca aatacaggaa
    17101 cagggagttc tggtgcagag ggtattacgt cgatacggtg ggtaagaaca cggcgaagat
    17161 acaggattac ataaagcacc agcttgaaga ggataaaatg ggagagcagt tatcgattcc
    17221 ctatccgggc agcccgttta cgggccgtaa gtaacgaagt tggatgcaaa tgtcagatcg
    17281 tgtgcgcctg ttagggcgcg gctggtaaga gagccttata ggcgcatttg aaaaacctcc
    17341 ggctatgccg gaggatattt attatgccac ttctcaatga tctgctcgat ttcagtgacc
    17401 atccgcttat gccaccgccc tctgcacaac tgtttgcaga gcaccttccc gtcgagtgga
    17461 tacagcactg tctgacgctt tctgcgcatg cgaccgttcg ccgccgtcgt ttaccggagg
    17521 acatggttat ctggatggtg gtgaccatgg cctttttccg caatgagcca attaccgatg
    17581 tggttcgccg tctgaacctg agcgcggatg gcgaatcggg gatcaacctg ctggcccgca
    17641 gcgctgtcac ccaggcgcgt cagcgcgtgg gggccgcccc ggaggaatgg ctcttccgcc
    17701 agaccgcaca gacatggggc tcggaacgtt acctgaagga tgactggcac ggcctgcagc
    17761 tttttgccat tgatggcgca cagttcagga cacctgatga acctgagttg cgtgaatatt
    17821 atggctctgc caacacatcc actgagcggc agagcgccta cccggtcatg cgtctggtgg
    17881 cgttgatgaa tctgggaagc cacattttgc tgaatgccgt gaccgcgcct taccggcgga
    17941 gcgaaaccgt gctggctcac tccatgctcg ccaccatccc ggataactcc attacgctgt
    18001 ttgacaagct cttttacagc gcagacctgc tgctgacgct gaaccagcag ggttgtaacc
    18061 gccactggct actgcctgcg tggaagaata tcgcggcaga aacagaagaa agttacggtt
    18121 ccggagacag gcttctgaaa ctgaaggtgt cgctgcaggc gaggaaaaag aatcctgcac
    18181 tgccagagtt ctggtacgcc cgggcggtga cttatgaggt gaacggtgtg gagaaaacgg
    18241 ggttgacatc actcccggca gaccgctaca aagcaaaaga ggtagcggaa ctttaccatt
    18301 cacgatggga aatcgaagtc gggttcagaa acctgaaaag cagcctgctg aacaatgccc
    18361 tggtgctgag aagccgtaag gttgagctgc tggaacagga ggtgtggggc atgttgctgg
    18421 cttacaacct gatacggcgt gaggcaacaa aagcggctga gaaacacaaa aaagcggcgt
    18481 cagaaatcag ctttaagttc gcgttccagt ttatcgcgac agaaatgata gtgctgggaa
    18541 atacagcatc accggggacc atcccgaaac gactggagca tctgcggggt gctctggaag
    18601 ttgtgttcat aacaaaacgc ccccgaccat caaggccgag ggcagttaag atatcaaaaa
    18661 cccgttatcc ggtgaaacgc agcaccgctc cgcttaagcg aacggcattg cggtgatgcg
    18721 ggatttgtat gccgtcgctg tggcatttct cggcccacat tgcgtaaatg gtggcgcaga
    18781 tatctggctc agggcatcgc tggtctggaa agccacagct gtcgccccag gcgttcacca
    18841 tcaacgaaaa ccggcacttg cgaagtcgct ctgattctgg agttacgttc tcagcgaaat
    18901 ctgggggcaa gacgtattca aagcgagtta aaacgacttc attcaatctc actggcaata
    18961 gcaacaatcc acaaagtcct ttgccagtat caggttaaac ctattgttaa atttcggcgg
    19021 ctacagagtc ctgaggctct actcttgtcg cacggcggca aactcactgg attttattga
    19081 ttgtgttatt gaagaaatgc ctttcccgat acagcgctct cagacagacc ggggaagaga
    19141 attttttgcc gttaaagtgc aggaaagatt aaaagaatac ggcatcaagt ttcgtccaaa
    19201 taagccagcg tcacctcatc ttaacggaaa ggttgaacgt tcccaaaaga cagacaaagc
    19261 agagtcttac gctaccgtag atctttctgc tgatgacctg aaggaactgc aggcagaatg
    19321 gcagcattac tataactggg agcgtcctca tagcgctcac aacggtaaaa ctccgatgga
    19381 acactacttt gaacttgctg agaaaacacc atactccgat gccgttcatg caaattgtca
    19441 gcccaatgaa ggtaaccgtc tcatttaaat cgtctttcgc ctccctttcc tgtttccgat
    19501 actaatgtcc attttcgcag taaaaggaca tttaagatga atgcacgaaa ggcggcactg
    19561 gcagataatc cggaattgat cctgcgtgtg ctacaactga gatttgacga gtcactgtcg
    19621 tacccgcgca tttctgcgca gaccagtgtc agcaaaacct gccatttttt ctctggtgag
    19681 gcgatttcac caggtattca ctgactggcc tctttccggg gaatattcct gcgtgcaact
    19741 ggcccgggct ctgttcccgg ggcgataccc ttcagccccg acagtaactc aggctgtgaa
    19801 aacagagaaa atccgccgga accgattttc accggagttt aaatggagac ttgttcagca
    19861 aactctttta cccggtgcct gtatcgcaca aatagctcgt gagaacggaa tcaacgataa
    19921 cctgctcttt aactggcggc atctctggcg taacggcggc ctgcagccga ccggcgaaca
    19981 tgaaacatcg ctacttcccg tgacgttaac gccggagccg gataataaaa tcccggcacc
    20041 agtgcagata cctgaacaga tgcccgacag tctgtgctgt gagctggttc tgccggccgg
    20101 aactctcagg ctgaaaggtg aactgacgcc agcgttatta cagacactta tccgcgaaat
    20161 gaaagggagc agccactgat gatatctctc cctgccggtt cgcgtatctg gctggttgcc
    20221 ggtatcaccg acatgcgaaa tggttttaac ggcctggcat caaaagttca gaacgtcctg
    20281 aaggatgacc cgttctccgg gcatctgttc atctttcgcg gacgccgggg tgaccagata
    20341 aaagtgttgt gggctgacag tgacggactg tgcctcttca ccaaacgcct ggaacggggc
    20401 cgcttcgtct ggccggtcac ccgcgatgga aaggttcacc ttactccggc tcagttatct
    20461 atgcttcttg aaggtatcaa ctggaagcac ccgaaacgaa cagaacgcac tggaatccgt
    20521 atataacccg ttgtaaagtg aggatatgga cacctcactt gctcatgaga atgcccgcct
    20581 gcgggcgctg ttgcagacgc aacaggacac catccgccag atggccgaat ataaccgcct
    20641 gctctcactg cgggtggcgg cttatgcttc cgaaatcaac cgactgaagg cgctggttgc
    20701 gaaactgcag cgcatgcagt tcggtaaaag ctcagaaaaa cttcgcgcta aaaccgaacg
    20761 gcagatacag gaagcacagg agcgaatcag cgcacttcag gaagaaatgg cggaaacgct
    20821 gggtgagcaa tatgacccgg tactgccatc cgccctgcgc cagtcttcag cccgtaaacc
    20881 gttaccggcc tcacttcccc gtgaaccccg ggtcatccgg ccggaagagg aatgctgtcc
    20941 ggcctgtggt ggtgacctca gtcctctggg gtgtgatgtg tcagatcaac tggagcttat
    21001 cagcagcgcc tttaaggtta tcgaaacaca acgtccgaaa ctggcctgtt gccggtgcga
    21061 ccatatcgtg caggcaccag taccttcaaa acccattgca cgcagttatg ccggagcggg
    21121 gcttctggct catatcgtca ccgggaagta tgcagaccat ctgccgttat accgccagtc
    21181 agagatatac cgccgtcaga gtgtggatct gagccgtgcc acactggggc gctggaccgg
    21241 tgccgttgct gaactgctgg agccggtgta tgacgtactg cgccagtatg tgctgatgcc
    21301 cggtaaagtc catgccgatg atatccccgt cccggtccag gagccgggca gcggtaaaac
    21361 ccggaccgcc cggctgtggg tctacgtccg tgatgaccgt aacgccggtt cggaaatgcc
    21421 ccccggcggt ctggttcgcg tactcaccgg accggaaagg tatccatcca caaaatcacc
    21481 tggccggtta cagcggtgtg cttcaggccg atgcttacgg tggttaccgg gcgttgtacg
    21541 aatccggcag aataacggaa accgcgtgta tggcccatgc ccggagaaaa atccacgatg
    21601 tgcatgcaag agcgccaacc gatatcacca cggaagccct gcagcgtatc ggtgaactgt
    21661 atgccatcga ggcagaagtc cggggatgtt cagcagaaca gcgtctggcg gtaagaaaag
    21721 ccagagctgc gccactgatg cagtcactgt atgactggat acagactcag atgaaaacac
    21781 tgtcgcgtca ctcggatacg gcaaaagcgt tcgcatacct gctgaaacag tgggaggccc
    21841 tgaacgtgta ctgcagtaat ggctgggtgg aaatcgacaa caacattgca gagaacgcct
    21901 tacggggagt ggccgtaggc cggaaaaact ggctcttcgt gggttccgac agcggtggcg
    21961 aacatgcggc ggtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtagagccag
    22021 aaaaatggct gcgttacgtt attgagcata tccaggactg gccggcaaac cgggtacgcg
    22081 atctgttgcc ctggaaagtt gatctgacct ctcagtaaat atcaatacgg ttctggcgag
    22141 ccgcttacat gaatggtcat attttaccac actccgattt gcagccgcca ggctgccgtg
    22201 gatccttagg gcggtgccat attacgagat aagatcaagc aatgagttaa gttataacag
    22261 gggcctgaat atataggggg gtaatacctt gaaaaatatc agtaaatttt taaaaaaaaa
    22321 agcatgcaag tattgatttt tatagagtgg caataaaaaa ttaataggaa acaaacgcgc
    22381 atgaaggtgg ttaatgctta catctaaaaa agaaatgcag agttctaaaa acaaacaaga
    22441 ggagaattta acgttattgt taactaatta catttcatat caaaatatag ttatatttac
    22501 gggggggaat cagtttaaga taagaaacaa gaaagaattc actgaataca caatagaacc
    22561 caactccttg ttttttttag caaaaaacac tcattgggac atggaagttg tcgggatcga
    22621 taatagtaat ccgtacagaa aaattataat tgatgatgca ttaattaagt tgctgcattc
    22681 aatatcatct gatgactctt gttatgtaaa aaaaaatata tttactgcga agctcaataa
    22741 aatgcaattg aatatagttt tcaatataat tgcagacata aaacactcgg gtaacaacaa
    22801 aaaaatattt aaaatattgt atctattatc tttttttaac gatcataatg atatagttaa
    22861 tgtcatcctt agtgcttcat ctaagagcat cgtagacagg gttataaaag taattgagtt
    22921 ggatatagcc aaaaactgga aactaggcga tgtcagtagt tcaa
//
LOCUS       DAZGJW010000068        22565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZGJW010000068 DAZGJW010000000
VERSION     DAZGJW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.068"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            44..541
                     /gene="ftnA"
                     /locus_tag="ACHKIK_002698"
     CDS             44..541
                     /gene="ftnA"
                     /locus_tag="ACHKIK_002698"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO9083842.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(579..818)
                     /locus_tag="ACHKIK_002699"
     CDS             complement(579..818)
                     /locus_tag="ACHKIK_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO9083843.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            1009..2220
                     /gene="tyrP"
                     /locus_tag="ACHKIK_002700"
     CDS             1009..2220
                     /gene="tyrP"
                     /locus_tag="ACHKIK_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO9083844.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2282..2947)
                     /gene="yecA"
                     /locus_tag="ACHKIK_002701"
     CDS             complement(2282..2947)
                     /gene="yecA"
                     /locus_tag="ACHKIK_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO9083845.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3304..4305)
                     /locus_tag="ACHKIK_002702"
     CDS             complement(3304..4305)
                     /locus_tag="ACHKIK_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9083846.1"
                     /translation="MSVRKIPSGKWLCECYPYGASGKRIRKQFATKSEALSYERRLMN
                     SRVGDEFQDGSGPRLSELIARWFEMYGKTLSSGAERKVKLEAICSRLGDPFASQFDKN
                     MFATYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKELAFLYVDEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQVLP
                     GRITFVKTKGKKNRTVPISPQLQAMLPQKRGALFSPCYEAFDAAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
     gene            complement(4311..4658)
                     /locus_tag="ACHKIK_002703"
     CDS             complement(4311..4658)
                     /locus_tag="ACHKIK_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HFO9083847.1"
                     /translation="MKVKKVQLLVTFLSMFSFSAVAMPFKTIERESFNGVWPFNTDEV
                     QLQCLDGNPYVMNFDDNKLYALTGLARIKGKTFGALPLDNNNPFWLDNDAAPGLKKSL
                     GDVTKAAFDLCDK"
     gene            complement(4688..5338)
                     /locus_tag="ACHKIK_002704"
     CDS             complement(4688..5338)
                     /locus_tag="ACHKIK_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083848.1"
                     /translation="MAVNSNGSNNRVAGRDFHEKNIQIERYDGSHTVNIAIPSNNDDD
                     DRPLLKAQRKELNSLVAAIAEASNTEAFIIWQKVHAEIGVAGIDDMTVNQYKTAESFL
                     HAMLERCKDHDACKTLVSLLLRNSEDCGLRQKLLRYCHINFGTGRLNDLTRSQLQSAL
                     SWLEQQSASSHTESSALPEVRLRASELIRLYPKEIIFFICVGVLVGGAISRVFFNL"
     gene            complement(5354..5758)
                     /locus_tag="ACHKIK_002705"
     CDS             complement(5354..5758)
                     /locus_tag="ACHKIK_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9083849.1"
                     /translation="MKNDIGQRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLAALSRFGLDVQYVVLGIHSPETYNDDEQELITRFRAASLDVKNAVIGALKGAI
                     SEKETQPSGRELNISGGNNRIAGRDYNETKGR"
     gene            complement(5848..5985)
                     /locus_tag="ACHKIK_002706"
     CDS             complement(5848..5985)
                     /locus_tag="ACHKIK_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083850.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSRDSRSASLGPVAVLRGMRDLV
                     S"
     gene            6057..6260
                     /locus_tag="ACHKIK_002707"
     CDS             6057..6260
                     /locus_tag="ACHKIK_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9083851.1"
                     /translation="MSGITININVNAPYVSLQKYAEITGIPLNTCKKMLADGRIIIRP
                     KRAKMEKPEVNLVAMLKDALANS"
     gene            6282..6632
                     /locus_tag="ACHKIK_002708"
     CDS             6282..6632
                     /locus_tag="ACHKIK_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO9083852.1"
                     /translation="MKKNTNNPYSKFRNGVERHVHHVATSASRSNSRYNLNETHATPD
                     GHAVKQIGEHTWLIEKAGIVVHRCPHNPFTGNRIFALSSGDNQFGQDFTLYEALRTVD
                     RLLRGQSFIKQAAL"
     gene            6643..6921
                     /locus_tag="ACHKIK_002709"
     CDS             6643..6921
                     /locus_tag="ACHKIK_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083853.1"
                     /translation="MTKDHAQGVFIRFIDFRGELLLRASAIDGVVPSEKNAATYVYLN
                     GTRLTVELPYQIIREIISEAEKSRQVNGDEPYIEIICMDSEAEIQKAD"
     gene            6933..7175
                     /locus_tag="ACHKIK_002710"
     CDS             6933..7175
                     /locus_tag="ACHKIK_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO9083854.1"
                     /translation="MDKEYKTLINKALERFYFRLSASGAHSERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            7172..7285
                     /locus_tag="ACHKIK_002711"
     CDS             7172..7285
                     /locus_tag="ACHKIK_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083855.1"
                     /translation="MSIFISWIVLIISVVCAIGIMRIINSVKKIERFFTGE"
     gene            7372..7575
                     /locus_tag="ACHKIK_002712"
     CDS             7372..7575
                     /locus_tag="ACHKIK_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083856.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQDPRK"
     gene            7899..8288
                     /locus_tag="ACHKIK_002713"
     CDS             7899..8288
                     /locus_tag="ACHKIK_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HFO9083857.1"
                     /translation="MIDAHDFTRWVRTQDTRLAPILRGLFDLYIRGRDNRARTTKPEN
                     VDTLYFTVDDCYRVDFTPHGLALHCLTPHGESLLAYYDSPASVFAAMLAHRTAGGCVS
                     LSEYTAEFNRLSTIFLQEWQRVTGYQP"
     gene            8285..11125
                     /locus_tag="ACHKIK_002714"
     CDS             8285..11125
                     /locus_tag="ACHKIK_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO9083858.1"
                     /translation="MSAFAWSWNEPRPAIDPARFTERRQETETDLQRAIRYYLEANKR
                     AQEEQEAKEEAFFAQSTVGKKLMASLEEAGQREKLAQSIISKRQATEQDPVARAFATL
                     KVLPVYLREPLSRHLSFLRKKQEADRQKGKKSWQAERYARGTLRKIFERLDRTDSRWL
                     TPGYRSLAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSGTFEKLCDGFGATDGELTM
                     DVTLKAYQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRK
                     LWLLRCEWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFT
                     IDLETVYYAGVSNPIHRKAEMMATMKGLELLAEARGDRAVFLTVTCPSKYHATTENGH
                     PNPKWNGATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFA
                     HPEEIDTIVSHTRDIAIQEDRHELGNDITPRFKVEYVDGSKGTPTSYIATYIGKNLDS
                     RAVDGIDPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRR
                     LASQMARNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLA
                     DELNDYGEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSTRENGFDLQGGP
                     AAPWTRGNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVNEWMRSLKRHERRALMRS
                     LRDKQAKNSSDEMQNWTQSRKQPRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLR
                     GGSLYVDGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLE
                     QLGASDPEAAAHLASTLQQDQNTMKTPVTVLSDMLRAIRDAVHAQRISETTERAHRKA
                     DLLRGSLTSGNKKQTETGLTNPVNEQKTCRDI"
     gene            11202..12161
                     /locus_tag="ACHKIK_002715"
     CDS             11202..12161
                     /locus_tag="ACHKIK_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO9083859.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNIIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            12166..12477
                     /locus_tag="ACHKIK_002716"
     CDS             12166..12477
                     /locus_tag="ACHKIK_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO9083860.1"
                     /translation="MTTPTRRISFYLKPTAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWAGDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            12541..13131
                     /locus_tag="ACHKIK_002717"
     CDS             12541..13131
                     /locus_tag="ACHKIK_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO9083861.1"
                     /translation="MSEINYQALRERYSPVPVPKCPICGEEMSIQRISGAQVVYACSG
                     YGDDGDFKIGRTLADEHYEKSRVTVLDVGDPEVLALLDWLETKDNRVAELEKIATDYA
                     LKFQKAQDALKYAALLHSRTAQQTNNFAVSLPDISEYFINDVFQPLRYERDVERAIIK
                     AGGKALWQEKHEDRTHQSCDVNRGWFSPLTTDKNNT"
     gene            complement(13622..14668)
                     /locus_tag="ACHKIK_002718"
     CDS             complement(13622..14668)
                     /locus_tag="ACHKIK_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9083862.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            complement(14668..16419)
                     /locus_tag="ACHKIK_002719"
     CDS             complement(14668..16419)
                     /locus_tag="ACHKIK_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO9083863.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDATVTGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKATHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTYRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            16574..17410
                     /locus_tag="ACHKIK_002720"
     CDS             16574..17410
                     /locus_tag="ACHKIK_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO9083864.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDRQKITSLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            17434..18486
                     /locus_tag="ACHKIK_002721"
     CDS             17434..18486
                     /locus_tag="ACHKIK_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO9083865.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKRINVIGVTDQKGEKILLDTTGPIARTNSSYDGIKRRNPNNVIDMK
                     SRQYQCEQVNYDTFISYPQLDTWAAHSDFQSRISTQIARQVALDRIMIGFNGTSHAYE
                     SDFHTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            18532..19332
                     /gene="gpM"
                     /locus_tag="ACHKIK_002722"
     CDS             18532..19332
                     /gene="gpM"
                     /locus_tag="ACHKIK_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO9083866.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAACTAHEEVLYRLRLAQA
                     RLKAIQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLWEI
                     VANQDIPDEVRAKLCKACAFARRGLTDADNMASSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKNKKTTRKPATRKTTATQKAKRG"
     gene            19434..19928
                     /locus_tag="ACHKIK_002723"
     CDS             19434..19928
                     /locus_tag="ACHKIK_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO9083867.1"
                     /translation="MSMVARTNPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAIAHVTRELEAWQQEQQAAGYQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILGRPRMF
                     AELC"
     gene            19928..20128
                     /locus_tag="ACHKIK_002724"
     CDS             19928..20128
                     /locus_tag="ACHKIK_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO9083868.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTRGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            20131..20454
                     /locus_tag="ACHKIK_002725"
     CDS             20131..20454
                     /locus_tag="ACHKIK_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO9083869.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGIPEDAVTFFGASIGFMGAEKARDKVIAIFDRRV
                     KERNE"
     gene            20451..20843
                     /locus_tag="ACHKIK_002726"
     CDS             20451..20843
                     /locus_tag="ACHKIK_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO9083870.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVTTGKSQTMNSRHLTGHAVDVVAYVGSQVSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            20840..21247
                     /locus_tag="ACHKIK_002727"
     CDS             20840..21247
                     /locus_tag="ACHKIK_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFO9083871.1"
                     /translation="MKLWPTLGVAFLLIAAWGTSMRLSWSLGRENARNEAQASTLKST
                     VDTLNIISAGVQDMQQVLAQLRADNQQRNQDGEVRREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHAGNASPLP"
     gene            21386..>22565
                     /locus_tag="ACHKIK_002728"
     CDS             21386..>22565
                     /locus_tag="ACHKIK_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO9083872.1"
                     /translation="MTSKNFALITAMTQAELTQKVNEHLAKGWHLQGETRVAYEPGTP
                     WYLMQAMVADGTTDISPDSPQHGSVPEWYYVVVLAGQSNAMSYGEGMPLPDSYDAPHP
                     RIKQLARRNTVTPGGKACAFNDIIPADHCLHDVQDMSALNHPNADLSKGQYGCVGQGL
                     HIAKRLLPYIPQNAGILLVPCCRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLYQD
                     LILRTKAALQKNPKNMLLAVCWMQGEFDMSAATYSQQPPLFAAMLKQFRADITEFNTQ
                     CHGGRAASVPWICGDTTYYWKNTYGTQYDTIYGAYKNRESDNVFFVPFLTDGSGNNTS
                     TNAPTEDPDAASEGYYGSASRTNKNWVSSNRQTHFSSWARRSIIPDRMATAILNVAGR
                     T"
BASE COUNT         5804 a         5993 c         5652 g         5116 t
ORIGIN      
        1 agtgacgcca cagcaacaaa tataaccttt gtggagcact atcatgctga aaccagaaat
       61 gattgaaaaa cttaatgagc agatgaacct ggaactgtac tcttcactgc tttatcagca
      121 aatgagcgcc tggtgcagct atcatacctt cgaaggtgct gccgcgttcc tgcgccgtca
      181 cgcccaggaa gagatgacgc atatgcagcg tctgtttgat tacctgactg ataccggcaa
      241 tttaccgcgt attaataccg ttgaatctcc gtttgctgaa tattcctcac ttgatgaatt
      301 attccaggaa acctataaac acgaacaatt aatcacgcag aaaattaacg aactggctca
      361 tgctgcaatg accaatcagg actacccaac atttaatttc ctgcaatggt atgtttctga
      421 gcagcatgaa gaagagaaac tgttcaaatc gattattgat aaattaagcc tggcaggcaa
      481 aagcggcgaa ggtctgtatt ttatcgacaa agaactctct accctcgaca cacaaaacta
      541 atgctctcag gcggcaggtt ttctgccgcc gtttcaaatc agtgacggca aatcttactc
      601 tgatcggtcg aaaaaccttc ttctgaaggt ataaatttgc cttttgctgc cagaaacgct
      661 accagctctc ccgcagtcat cccttctgcc gagcaggtgt gaaaacgtgc ctgttcacca
      721 aaacgcgctt taattgctgc ttccagactg gcatgcgtat attgctcgcc tgattcaatc
      781 atcatattta acacttcatg accgtgaata gagtccatcg tccctcctca aaaaaagcct
      841 agcgtagcga ttgccgctta tgaagacttt gcgccagcgc aggactgaat gctttttatt
      901 gtacatttat atttacacca tatgtaacgt cggtttgacg aagcagccgt tatgccttaa
      961 cctgcgccgc agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga
     1021 accctgggaa gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg
     1081 ccgctggctg cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg
     1141 gcgttgatgt gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat
     1201 accggtcttg gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc
     1261 ttcagtatga tgttcttaat gtatgctctg actgcggcat acatcagcgg tgccggtgaa
     1321 ttgttggcct ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg
     1381 ctgttgttca cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta
     1441 tttaaccgtt ttctgttcag cgccaaaatt atttttctag tggtgatgct ggtattgctg
     1501 ctaccgcata ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggctctg
     1561 tcagcaatcc cggtgatttt tacctcattt ggttttcacg gtagcgtgcc gagtattgtc
     1621 agctatatgg atggcaacgt tcgtaagcta cgctgggtgt ttataaccgg tagtgcgatc
     1681 cccctggtgg catatatttt ctggcaggtg gcaacgcttg gcagcattga ttcaacaacc
     1741 tttatgggat tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa
     1801 atggtggcct ctccgcatgt tgagctggca gtgcatttat ttgctgattt agccctcgcc
     1861 acgtcatttc tcggcgttgc gttaggctta tttgattatc tggctgattt atttcagcgt
     1921 tcaaataccg ttggtggacg gttgcaaact ggtgcaatta cctttctgcc gccgttggcg
     1981 tttgcactgt tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg
     2041 gcggtactgg cattgattat cccttcgctg ttgacctggc aaagcagaaa gcacaatcct
     2101 caggcgggtt accgggtgaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt
     2161 attgctgtga ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga
     2221 tcagatagcc tcaaattcct tattgggtgc cagaattaac gctgacaccc aatttgacct
     2281 cttaatgcag gcagcactgc ttaaatttct taccactacc gcacgggcaa ggatcgttac
     2341 gccccggttt ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca
     2401 tccagtacgc atgtagatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg
     2461 cttctggcga cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg
     2521 cctccagcgc tggttttaac gaatcgggca acgttgacca gtcagaaagt gccacgcccc
     2581 gcatatagcc aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca
     2641 agccgaataa cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca
     2701 tatgttgaaa agccagattc ataaagcgcg tcatctcttt ctctgacgcc cagcgcggca
     2761 catagtcagc cccaccccac acggcaacca gccactgttc cggttcaatc tcttgcggag
     2821 aactcaacac cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag
     2881 tgttgtattt ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg
     2941 ttttcatacg cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc
     3001 gtatcagaga tactttttga gtggctttgc tggtgattaa aaattaggga gggtgtaacg
     3061 acaagttgca ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattgcttt
     3121 gattttattc ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa
     3181 cgccgaggga ttttaaaaac tatcaaccac catttataaa tcataacctt atgattttat
     3241 taactttgaa aatggctcta tacaacgcca tttaaatcta ttgtcacttt ttatcgccac
     3301 tttttaccct ctattgtcaa aagggttcaa ttcaacagca gcctctaaat gacctggagc
     3361 aaaatgcgca taacgcatag tcatttttat atcgctatgc cccagtattt tttgcaacac
     3421 aagaatattt ccgccccgca tcataaaatg actggcaaac gtgtgacgta gcacatgagt
     3481 taattgccca tcaggaagct cgatcttcgc tctcttaatt gcagcgtcaa aagcctcata
     3541 acatggtgaa aatagcgctc ctcgtttttg aggaagcata gcctgcaatt gaggtgaaat
     3601 cggtacagtg cggttcttct ttcctttagt tttaacaaat gtgattcgac cgggcagtac
     3661 ttgagattgc tttaatcctt cagcttcact ccaccgagca ccagtcgcaa gcccaatacg
     3721 gacaacaacc cccaaatctt tattccgtga ctcatcacac gcaatcagaa ggcgttcaat
     3781 ctcatctaca tacagaaacg ccagttcctt ttcttcctca cgaaacttgc gaataccagt
     3841 cagggggttt tcaccagacc actccccaag gcgcttcagt tcggcaaaaa cagcatgtag
     3901 atatgactgc tcgcgattaa cggttgcttc actaagtttt ttcttcccct tgggattcca
     3961 ttctcctgat agccttcttt cccgataagt agcaaacata tttttgtcaa actgagaagc
     4021 aaatggatcc cccagcctgg aacaaatcgc ctcaagtttg actttgcgct ctgcaccaga
     4081 ggacaaggtt ttaccgtaca tctcaaacca acgagcaatc aactcagaaa gacgaggacc
     4141 agaaccatct tgaaactcgt ctccaactct actattcatt aaacggcgct cataagagag
     4201 cgcctcactt tttgtcgcaa actgtttacg aatgcgtttt cccgatgccc cgtagggata
     4261 acattcgcaa agccatttac ctgatggaat cttacgaacc gacattttag ttacttatca
     4321 cataaatcaa atgcagcctt agtgacatcc cccagactct tttttaaccc tggggcggca
     4381 tcattatcta gccaaaatgg attattgtta tctaacggta acgcaccaaa tgttttacct
     4441 tttattcgag ctaaacctgt aagcgcatat aacttattat cgtcaaaatt catcacataa
     4501 ggattaccat caagacactg taattgaacc tcatcagtat taaatggcca taccccattg
     4561 aaactctcac gttcaatagt tttaaaaggc attgcgacgg cggaaaaaga aaacatagat
     4621 aaaaaagtaa ctaatagttg aacctttttt actttcatat cattacctca atttagctca
     4681 agtaagatta caaattaaaa aacaccctag aaatggcacc gcctaccaaa acccctacgc
     4741 agataaagaa tattatttct tttggataaa gtcggattaa ttctgaagca cgaagtcgaa
     4801 cttctggtaa ggccgaactc tctgtgtgac ttgatgccga ttgttgctct aaccacgaca
     4861 atgcagactg taactgagaa cgagtaagat cgtttaaacg tcctgtaccg aaattgatat
     4921 ggcaataccg cagaagtttt tgtcgaagtc cacagtcttc actgttacgt agtaataaac
     4981 ttacaagagt cttacaggca tcatgatctt tacatcgctc aagcattgca tgcagaaaac
     5041 tctccgctgt tttatattga tttactgtca tatcatcaat accagctaca ccaatctccg
     5101 catgtacttt ttgccaaata ataaacgctt cagtattgct agcttctgca atagcagcaa
     5161 ccaagctatt tagctcctta cgctgagcct taagcaaagg gcgatcgtca tcatcattat
     5221 tcgaagggat tgcgatattg acggtatgag aaccatcata tcgctctatc tgaatattct
     5281 tttcgtgaaa atcacgccca gcaacgcgat tgtttgaacc gtttgagttg acggccatgt
     5341 cacctcccta ctatcaccta cccttagttt cgttatagtc acgaccagcg atacggttat
     5401 taccaccaga aatatttaac tcacgtcctg atggctgagt ttccttttca ctgatcgcac
     5461 cttttaaagc cccgatcacc gcgtttttca catctaacga agctgctcga aagcgagtaa
     5521 tcaactcctg ctcatcatcg ttataagttt caggtgagtg aattcccaac acaacatact
     5581 gaacatcaag gccaaaacga gacagcgctg ccaaatacgc agcatcagga aagctatctc
     5641 ctttctcata tctaagctga gttagctttt tgactccacc aatgtcgctc atggcaactt
     5701 gactaagtcc caatctttcc ctttcctcac gcaaccgctg accaatatca tttttcatac
     5761 aaaaaccttg acaggtatct tttttgatac caaaatgatt tcacgagcta ttagatgatc
     5821 acaatatacc actatgaaac aagttcttca cgataccaga tcacgcattc cgcgtaacac
     5881 cgccacaggt ccaagactgg cacttcggct gtccctcgag gagcgagccg tcattgatga
     5941 aatggcagct aaagaacaac gctcatcctc taacatggcg cgcatgatct tccttcgcgg
     6001 cctagagcta acccagaaag aacaaaacaa atcttcctga tcaggaggct agtgggatgt
     6061 caggtataac catcaatatc aatgtgaatg ccccctatgt atccctgcag aaatatgcag
     6121 agataacagg tatccctctt aatacatgca aaaagatgtt ggctgacggt cgaattatta
     6181 tccgccccaa acgcgccaaa atggaaaagc ctgaagtaaa ccttgtggcg atgttaaaag
     6241 acgctttggc taacagctaa aacaatgaac agagcatcat catgaaaaaa aacactaata
     6301 atccatactc caaatttcgt aatggcgtag aacgccatgt acaccacgtc gctaccagtg
     6361 catcacgtag taacagccgc tataacctga acgagacgca cgcaacacca gatgggcacg
     6421 ctgtaaaaca aatcggcgaa cacacctggc tgattgagaa agctggaatc gtggttcaca
     6481 gatgcccaca caatccgttt accggaaacc gcatttttgc attaagcagc ggcgataatc
     6541 agttcgggca ggatttcaca ttgtacgaag cactacgcac agtcgatcgt ctgcttcgcg
     6601 ggcaaagttt tattaaacag gctgctttat aacaggtgct ttatgactaa agaccatgca
     6661 caaggtgtat ttatccgctt tattgatttt cgcggtgaac tgttattacg cgcatcagct
     6721 attgacggag tagttccatc tgaaaaaaac gcagctacct acgtttatct gaacggtaca
     6781 cgcctgactg tagagcttcc gtaccagatc attcgcgaaa taattagcga agccgaaaaa
     6841 tctcgtcagg ttaatggcga tgaaccctat atcgagatta tctgcatgga ttcagaagct
     6901 gaaattcaga aagcagatta aagggtgttg cgatggataa agaatataaa actctcatca
     6961 acaaagcact tgagcgtttt tattttcgtt taagcgcatc cggcgctcat tcagaacgtg
     7021 cagcccgtga ctcattgacc agggctatcc ggagtctgta tgacgtagct ttttacgctg
     7081 acgatctgga tgcacttaat gaactttctg agcttatctg cgccgcagaa tgcggggagc
     7141 atattgagcc gtacaaactg ggaaatatcg catgagtata tttatatcct ggattgttct
     7201 tattatttcg gtggtttgtg caattggtat tatgcgaatt attaattcag taaaaaagat
     7261 tgaacgcttt ttcactggcg aataaccgca taaataaaac cccagcttaa ataaaaaaat
     7321 gtgaacacaa tctgcattcg cagaggtatt cacacacacc aaggaggcgt aatggcaatt
     7381 aagcattttc ctgtcgttcg tttcacctcc agaggacgtg aatacgaagt cgacgaacgt
     7441 ctgattacca caatcgacaa acaccgctca gaaaaggatg cacaccacat ctatctcact
     7501 gacggcactt acttttgcgc cactaatgtg gcgcgggtga atcttatccg acaggtacag
     7561 gatccacgca aatgagtagg agaagaatca ctcgcagaca tcaccgaaca cacctgagtt
     7621 cctcagcaac gctaaaggca cttattcaaa gcgagatcgg tgatttcttc gcgggagttg
     7681 gctcaccagg cgaaccagaa acaccagaag cgatgcagcg tgagctcatg atacgcatag
     7741 ataacacttt tgatttcttc tacagcatgc acggaattaa acagaaatga acctcaagcc
     7801 agcaataact actcgtagaa cgtcaattcc tgtaaccgac cgcttctgag ttttttggca
     7861 ggaagccttc gcacatctgt agtaaagaga attgcagcat gattgacgct catgacttca
     7921 caagatgggt gcgcacacag gacacccgtc tggctcccat tcttcgggga ttatttgatc
     7981 tctacattcg tggtcgtgac aacagagcac gcaccacaaa accggagaat gtggataccc
     8041 tttatttcac agtagacgac tgctaccgcg tggacttcac accacacgga ctggcgttgc
     8101 actgcctgac accgcacggc gaatcactgc tggcgtatta cgactccccg gcctccgtat
     8161 ttgcggcaat gctggcgcat cgcactgctg gcgggtgtgt ctcgctgagt gaatacaccg
     8221 ctgaatttaa ccgcctttcc accatcttct tgcaggagtg gcagcgcgtg acgggatacc
     8281 agccatgagt gcgtttgcat ggagctggaa tgaaccacgg ccagccattg atccggccag
     8341 atttacggag cgcaggcagg aaactgaaac cgacctgcaa cgcgccatcc gttactacct
     8401 tgaggcaaac aaaagggcac aggaagaaca ggaagcgaag gaggaagcct ttttcgcaca
     8461 atccaccgtg ggtaaaaaac tcatggcatc ccttgaggaa gccggacagc gtgaaaagct
     8521 ggcacaaagc atcatcagca agcgtcaggc aacagaacaa gacccggtgg cccgtgcttt
     8581 tgccacactg aaggtgcttc ccgtttatct gcgtgaacct ctgagccgcc acctctcttt
     8641 tctgcgcaag aagcaggaag ccgatcgtca gaaaggcaaa aagagctggc aggctgaacg
     8701 ctacgcgcgc ggaaccctgc gcaaaatatt cgaacgtctg gaccgcaccg acagccgctg
     8761 gctgacaccg ggctatcgct cccttgccgg acgcgaacgc ctggacgatt tgctttacct
     8821 gccgcagctc aacaaacacc agatacagac gctggccacc atgacggcgg cgatgttcag
     8881 tggcactttt gaaaaactct gcgatggctt tggcgcgacc gatggcgagc tgaccatgga
     8941 tgtaacgctg aaggcgtatc agatgctggc ccgcatggcg ttacacctgc acgctatgcc
     9001 tccacattat gacgctctga caacagacaa agaccggagg aatgaaccgg acacggagct
     9061 gctgccgggc gcaatccttc gcctgacctg tgcggaatgg tggaaacgca aactgtggct
     9121 gttacgttgc gagtggagag aagaacaact ccgcgccgcc tgtctggttt ccagaaaaac
     9181 atcaccctat ctgagccagg acgcgttaag cgagtttcgc gcacagcgcg agaaaacacg
     9241 cgatttcctg aaaagtttca tgctggaaaa cgaagacggg ttcacgattg atctcgagac
     9301 agtgtattac gcgggagtaa gtaacccgat tcaccgtaag gcagaaatga tggccaccat
     9361 gaaggggctg gaacttctgg ccgaagcccg tggcgacaga gcggtgtttc tgactgtcac
     9421 ctgcccgtca aaataccacg ccacaacaga gaacggtcat ccgaatccca aatggaacgg
     9481 ggccaccatg cgcgactcca gcgattacct ggttaacacg ttttttgcgg cggtccgcaa
     9541 gaaactgaac cgcgacggcc tgcgctggta tggcatccgc acggtggagc ctcaccatga
     9601 cggcaccgtg cactggcata tgatggtctt tgctcatccg gaagaaatcg acaccattgt
     9661 gtcccacacc cgcgatattg ccattcagga agaccgccac gagctgggca atgatattac
     9721 tccgcgcttt aaggtggagt atgtcgacgg ctcaaaaggc acgccaacca gctacatcgc
     9781 cacctacatc ggaaagaacc tggacagccg cgccgtggat ggcatcgacc cgaaaacggg
     9841 caagccacgc gttgaccacg aaaccggaaa atcaatggcc gagagcgtgg aacgcgccat
     9901 cggctgggca cgccttcacc gggtccgcca gttccagttc tttggcatcc cctcccgtca
     9961 ggtatggcgt gaactccgcc gccttgccag ccagatggca cgcaacccgg aaggcccaca
    10021 acggctgaag gatgatgcaa tggatgcggt actcgctgcc gctgatgccg ggtgttttgc
    10081 cacctacatt gagaaacagg gcggcgtact tgttccacgc aaagactacc tgattcgcac
    10141 cgcctacgac ctcgcagatg agctgaacga ttacggcgaa cagagcgtac agatttacgg
    10201 gatctggtca ccactcatcg gggaatcctc ccgtgtgtgc acgcacccgg ataactggaa
    10261 gctggtaaga cgtaaaccgg aagcggaaga cagcacccgc gaaaatggtt ttgaccttca
    10321 gggcggccct gccgcccctt ggactcgtgg caataactgt ccccgtgtac aggaaacgga
    10381 caacaacggg acagaacagc cggaagaaag gccagcaccg tggccgcagc ttcctgacgg
    10441 cgttgacgtg aatgaatgga tgcgctcact gaaacggcac gaacgccggg cgctgatgcg
    10501 ttcgctgcgt gacaaacagg caaaaaacag cagtgatgaa atgcagaact ggacacagag
    10561 ccgcaaacag ccacggcctt tgcctgataa ccacgagtta ctcgctaaag aatggcggga
    10621 gtctgctgaa tctctcggcc tgcatatcgg tgaacagcag atgcagcacc tgttacgggg
    10681 cggcagtctg tacgttgacg gcagcatcat tgcaccgcag ggatttgaaa ttgtacgcaa
    10741 accagatacc cgcccggaca gccgaatcac gcaactctgg cagcgcctga gccgtaatca
    10801 cggcgtaagc agcacggaga tccgccataa cccggtcgcc agctatctgg aacagctggg
    10861 ggcatcagac cccgaagccg ccgcacacct ggcatccaca cttcagcagg accagaacac
    10921 catgaaaacc cccgttaccg tgctttctga catgctgcgc gccatccgtg acgcagtgca
    10981 cgcacagaga atcagtgaaa ccactgaacg cgcccaccgc aaagcagacc tgctgcgggg
    11041 tagcctgacc agtggaaaca aaaaacagac agaaacggga ctcacaaatc ccgtaaatga
    11101 gcaaaaaacg tgccgcgata tatgaagcgc gcacaaaaca ggcaaaagcg ggatttaaaa
    11161 atcctgtaac cgattaatta atcaacacaa ggaaaagcga catgaaaatt tgtatcgacg
    11221 acggctccac caacatcaag ctggcgtgga ctgagaacgg cgaacgccgc aacgccatca
    11281 gcccgaacag cttcaagtcg gaatggtctg cgccgttcgg tggcacgcag cccgcgaact
    11341 acatgcttga tggcgtgcgc tatggttttg atccggtcag cgatcgcttt gtccagacga
    11401 ccgacacgca ataccagtac agcgatgtga atataattgc cattcatcac gcgctggtca
    11461 aatcaggcat cacaccacag gaagtggatg tggttgtcac cctgccactg agcgagtatt
    11521 tcgatacaaa cgcacagccg gacatggcca acatcaaccg caaaaaagcg aacgtcatgc
    11581 gcccggtgga gtaccagaac ggcgaagcat tcactatccg taacgtacgg gttatgcctg
    11641 aatccattcc ggctggcttt aaggcactgg ctgacatgag tccgtttgaa tccctgctga
    11701 ttgtggattt gggcggaacc actctggatg tggcaaaggt tcaggggcaa ctggcaggta
    11761 tcagccaggt gttttgcgat ccacacgtgg gcgtttctct gatggccgat gccgtactgt
    11821 cggtgatggc cactaacggt atgcgcacca gtcaccacat cgccaatacc attatcgaac
    11881 atcgccatga tgaagcctgg ctgcgccagc acatccacaa tgacgcgcat tacgccagcc
    11941 tgatggcggt tattcgtgaa aaggaagaaa cactgaaaca acgcgtgatc cgcgcgctgg
    12001 cgggtttttc gggttacggg cgggtgatgg ttgtcggtgg aggggcggag attgtggcac
    12061 ccgctatccg cgaagcctgc ggagttaatg cgactttcat cgcggacggg gtgccacagt
    12121 ttgctctggt taatgggctg tacgcaatgg acaaggagta aaccaatgac gactccaacc
    12181 agacggataa gtttctatct gaagcccacc gccgtcaaga acgaaggaga agcatgcgcc
    12241 tggctggaca gccttacacc agaagcccgc aaaagcggcc aacgcgtggc ttttctggcc
    12301 gggctggcac ttctgaaaat gaatccggca gaggcttatc gactggctgc atgggctggt
    12361 gatgaagcgt tatcagtgac acaaaccagg acagaacgcc ccgcatcaca gccagtatca
    12421 accgcacaga taaccagtca gatggccgga aatatccggg cgttatttcc tgaataacac
    12481 aacatcaggg cgagttcgcc ctgctctcca cctcagaaca taaacaagga gaacgactta
    12541 atgagcgaaa tcaactatca ggcattacgt gaacgttatt cacctgtgcc agtaccgaaa
    12601 tgccctattt gcggcgagga aatgtcaatt cagcgaatat ctggagcaca ggttgtttat
    12661 gcctgctccg gttatggtga tgatggagat ttcaaaattg gccgaactct tgccgacgaa
    12721 cattatgaaa aatcacgcgt aacagtgttg gatgtcgggg atcctgaagt attggcgcta
    12781 cttgattggc tggaaaccaa agacaaccga gtcgctgaac tggaaaaaat cgccactgac
    12841 tatgcactta aattccagaa agcacaggac gcattaaagt atgccgcttt gctgcatagc
    12901 aggacggcgc aacaaacaaa taattttgca gtatcgcttc cggacataag cgaatatttc
    12961 attaatgacg tatttcagcc cttgcgatac gagcgggatg ttgaaagagc catcataaag
    13021 gctggcggaa aagcattgtg gcaggagaaa cacgaggaca gaacgcatca gtcctgcgat
    13081 gtaaatcgtg gatggtttag cccactgacg acagataaaa ataacacctg atccccctca
    13141 aaccagggcg ataatcgcgc atcgccctgc tgcacaatag tgcacaagtt tgcacaattt
    13201 ttttgaacga ctttttaccc ttccggcccg catggcggct ggatccgtca aggatccgtg
    13261 cgtgcacaaa aaaacgcgct ttttctgcgc gcaggtgacg ggggaacagc ccgcgtttca
    13321 gggggtaaat agcatcccct gaacgatgtc gcagcgacac aacagaatgg ctgtatttct
    13381 cacgctgagc gtgaagaata cgtgagggat tctgatttga tggggtgaaa ggtaaggccg
    13441 tcaaaatcgc actgaggcgg cgagaacatg cagtcaacgc ggtgggattg cgtaagagtc
    13501 tgaccgtcga tggtggcgat aaactggaag gcgtcgtgaa attatctgac tgatacagga
    13561 gctggagagt cggggcataa attttttatg ccccggcgaa gcagcagaca agcgaagcgc
    13621 gtcaggatgt gggctgggtg tctaacagtg cgtaagggtt aaagcggatc acctcttcgc
    13681 caagccagtc attgatgtgc ttcatggcct ccatgacggg catcagctcg ttaattgcgt
    13741 aaacccgcgc ggccttctcc acatcaccaa acgcactttt ttcacccggc atcgccccca
    13801 tcagttgcgg cggaacgcgg tgcgcagcca gcacatcatc acgggatgcc gccttaacat
    13861 tcatgaactc atcctttgcg gtgatctgct ggaacggcaa aatttgcacc ccctctttgc
    13921 ccccgttggg cgcatgaatg agcacgtttt taaacgcacc accaccacgc gcaccctgta
    13981 gcgtttcttt cagggagtcc atgctttcgc ggtttacctg cgctgcaccg atgtagatga
    14041 tgcacccggc gtgggatccg ttgtcgtaat acagttttct gaacatgtcc gccgaatgag
    14101 acaggctggc cgagagtaat gcgccgagat attccggcat gccgtagatt tcctggttaa
    14161 tgtccggatt catcaggtgg cacactttgc ctgggcgaaa ctgaaacgca tccttgccat
    14221 cctgcacata ccaccatgat tcaagatcgc ttccgcgtcg catgtatttc gccagggcgt
    14281 gccgtaattt aagcggttcg ccgagcatat tgcttcgaag ctcaaggaat gcgttaccga
    14341 acacaaacca gtccagcgcc agcgccgaga aatcctgccg ggaaagcagc gggtgcggga
    14401 tgtagcaacc gagcaataca ttgcgcttaa agtaaagcgc agactgatgc caggacgttt
    14461 gccgggcggc tcttgccaga ccgtaccagt ccaccggggt ttcataccac cgcccgttat
    14521 cagcacagta catattgtcc agcaggtcat gcccggtcag gcgataagga ccatcaaatg
    14581 tgaatgcact gagcgatgat tctttcctga gcgcatcagc gagatcaatg cgtgaactca
    14641 tgcgcacttt tttatttttt ctgctcatca gaactccata accgtgaaac gctcgttttc
    14701 tccttcgccg ccaatcggtt cgttaatgac agcaagcatg gttgcccacg caaggtcgcc
    14761 gtggctgatc cccctcgcgc ggtccgtttc gtaagtgata aagccgcccg gtgttttcac
    14821 cttacgcacg gcgttaaagg ccgcgaccag ctcgcgttcg gcgcgatcgt attcccaccg
    14881 cccggcacgc attatttgca gcattttcag taccagcgac cgttttgatg acagcgtgaa
    14941 ggtgtacgga atagcagcag ggaaaaaccg tttcactatc tgataaacag cctccccgtt
    15001 cccgcccgtc acatcaatgc cgatgtgttc cacgttgtag cgatacgtga actcttcaat
    15061 gactctggcc tgttcttcaa actccagccc ctgaacgcgt cgcgtctcca ccgttcgaaa
    15121 acggccacca ggaacagccg gaggaaccac cacggacaca gcgccgctgt cgccgttgcc
    15181 actactgccg tttgcgtcat acccaatcca taccggacga ttccccatcg ggcggggagc
    15241 aaaaggtttc cagtctttcc agtcgtcgta tccgtcaaca ccgcagccaa tcaggatatt
    15301 caggttaaat gccgattccc cttcgcggac aaactcacac atatagagat tgaggaactc
    15361 gtcttcggtg ttttcatcac gaatttcgtc gatatcggtg tgtttccagc cgtgattgac
    15421 cacatcttcc agcgtgacaa tttgccgcca cgtccggtca gggcagataa gcccgttatg
    15481 cagcgttttc cagtccacag aaaaacgctg gcgtttatgc gtggcctttt tctcgttcca
    15541 gcggtcgccg ttccagtagg cgtatgcctc gtgcgtttcg gtggatggcg tggagaagta
    15601 ggtgcgccgc agtccgctga gggttgccat agcgccagcc accttgcgca gttcagcaaa
    15661 gcgactgacc cagaaaaatt catcaaaata aaaattgccc gtataggact gtgccgacgc
    15721 agcagaagtg ccgagaaaat gcagctctgc gccgttggag aggatgattt tatcgccccc
    15781 tttcagctcc acatcaactt cagccgcggc cttctgaata atgcttttaa actggaacgc
    15841 ctgacgacgc gacgcagaca aaaaaatctg gttacgctgg taaggttgcg ccacatcgtc
    15901 acgcagcgcc atcagcagag cttcctgtgc aaaataccag gtcgccccaa tctgtcggga
    15961 tttcaggatc atcctgttac gtatcccggc ttccctgcaa agggtcaggg agtcaaacca
    16021 gccccgctga tgccactcca gcctgctgat gattttttcc cgcagtgcgg caatctgttc
    16081 cggcgtgaaa tgatttttga gttttttcgc ccggcctttc tttcctgtga ccgtcgcatc
    16141 cggctggcca tcatgcagtt ttttaagctg ccgggtcagc aggtctattt ccttaaagtc
    16201 accgcctgtt ttattctgtt tttcagtaag ctggatgagg cgcgcatcga tggactgcgt
    16261 gacacgctgc acgggtggcg tttcatccca ctggtcgcgt tttttccacg cataaatcgt
    16321 gttcgggttt attcccatca gacgtgatat ttctgcgggc ggataaccct gccagtaaag
    16381 ttgccgcgca cgctggcgca caaaagcgtc ctgaatcatt gctccccctg agtaattaca
    16441 ggaagattac ccgcgcgcga aactgttctc cttaaccccc tgttctggcc gttttcttac
    16501 aacaaaatcc ctttgtatca gcctgttacg ctttgccatc atgactgaag aaccagtcag
    16561 aggggcaaaa actatggcta atgaaaaaaa gacatcccgc aaaaagtttc gcgtggctgt
    16621 ctccggatca actgttgatg gccgtgaaat cagtccggtg catctgcgtg aagccgccga
    16681 gaacttcaac ccggatgttt acgctgcccg cgtgaacgtt gagcactatc tctcgccatg
    16741 cccgtcaagc gaattttccg caatgggcga tgtcaccgcg ctgagtacgg aagatattac
    16801 ggaaggcccg ctggccggac gtactgcgct gtatgcagaa atcgaaccga ccgagcgcat
    16861 gaagcagctt gtcgctgacg gcaagaaaat ctattccagt atcgaactgc acccgcagtt
    16921 ctccgttaac gggcgtgcct atctggtcgg gctggcgatg accgacaccc cggcaagcct
    16981 gggcactgag cgcctgaaat tcacggcaca gcagcgtcag gcggtgatga cgttcaacag
    17041 tatccagggt gaagcaccgc ttatctccga agccatcgag tctgaaatca tcgaaatggc
    17101 agaacaacgc caggaagaag gcacccagtg gtttaaccgc gtaatgggga ttattggtcg
    17161 tggccgcaaa gcggatgacg ccagtttctc ccgtattcag gaagcggtgg aaggcgttgc
    17221 aacatcacag gccgacatta tcgaccgttt taatgtgctg gaaacccgcc atcagcagga
    17281 ccgccagaaa atcacgtcac tgaccacaga gctgacagca ctgaaggaaa aactgcgcac
    17341 gcaggacggc gatccgcaga accgcttcac cgcaacgggc gcagcctccg accagctggc
    17401 tgacttctga taagacaaag gagcaaattt tttatgaatc tggtgatgtc agatattacc
    17461 cgcaacaagc tgggttgcta tatggcgcag caggcgtcgc ttaacaatat cccggtatct
    17521 gcactggtat cgcgatttac cgtggaaccc gcggtgcagc agcgttttga aaacgcctca
    17581 aaggaaagta ccgaatttac gaaaagaatt aacgtgatcg gcgtgaccga ccagaaaggc
    17641 gaaaaaatcc tcctggacac caccgggcca attgcgcgca cgaatagcag ttatgacggc
    17701 atcaaacgcc gtaacccgaa taacgtgatc gatatgaagt ctcgtcagta ccagtgcgaa
    17761 caggtgaact acgatacctt tatttcgtac ccacagcttg atacctgggc ggcccacagc
    17821 gattttcagt cccgtatcag tacacagatc gcccggcagg tagcgcttga tcgcatcatg
    17881 attggcttta acggcacatc ccacgcctac gagtctgatt ttcacaccaa caagctgctt
    17941 caggacgtta acgtgggctg gctggagcac atcagaaccg atgccagcga gcgcgtaatg
    18001 aatgacgtga cgctgacctc ccgcaacatg gacaacactg tggcgcacgc gggtaagtat
    18061 gcgaatgccg atgctctggt acaggatgcg cgctcatccc tgctggatga atggcacaag
    18121 gaagctgacg acctcgtggt gattatgggg cgcaacctgt ttaactcgct gcgtctgccc
    18181 gtgctgaaca gcatcagcgg ccagaatccc aatgcggaat tacttgccgg gcagctcatc
    18241 ctgtcatcgc gcaccattgg cgggctgggc gtgttccttg cgccgttctt cccggatgca
    18301 acgatgctta tcacctcgtt caacaacctg tcgatttact ggcagaaagg ttcaatgcgt
    18361 cgtctgatga aagacgagcc ggaatacaac cgcatcgcca cctaccagtc catcaatgac
    18421 gcttatgtcg ttgaagacta tggcaagtgc gcgatggtca ctggcctgaa gttcgccgac
    18481 agctaatcaa ctcacggcgg gcatcatgcc cgcctgtaac ggagagaaaa aatgattact
    18541 cctgcacaac aacactggca gaacgtgatg gcacagcgcg caggccgggc gaatgaaggt
    18601 gtggaccacg ccgcgtgtac cgcgcatgaa gaagtgctgt atcgtctgcg tctggcacag
    18661 gccaggctaa aggccataca ggccagaagc gcgaaagccg ccatcaaaaa agagttgttg
    18721 ccggactttt ccggctggat tgagggaacg ctggaggctg acggcgggca acaggacgaa
    18781 gtaattgcca cgctgatggt gtgggcgatt gactgcggcg atcttccgct ggcgctgcgt
    18841 attggtgcat atgtggtccg tcacaacctc atcatgccgg ataactttgg ccgtactgct
    18901 gccacggtac tgaccgaaga aatctgcaac ccggtactga cgcaggctgg gacggatgcc
    18961 gacgcggatt tatccgcctt tatcgaacca ctggacacac tttgggaaat tgtcgccaac
    19021 caggacattc cggacgaagt gcgcgccaaa ttatgcaagg cgtgtgcctt tgcccgtcgt
    19081 ggcctgaccg atgcagacaa catggcctca tcactgaagc tgctgcgcga agcgatgcac
    19141 ctgaacccga acgcaggtgt gaaacgcgag attgcaaccc tttcccgcgc cctgaaaaaa
    19201 gccgattccg cagccgaacc agaagacgcc agcgcacagc aggcgcagga cgaaagcagc
    19261 aaaaataaaa agacaacgcg gaagcctgca acacgaaaaa ccaccgcgac gcagaaggca
    19321 aagcgcggtt aacgactgac cccgtcagcg ggcggcgtgc gcggtgttcc ggtttgactc
    19381 cgtgaccgtt tacaccgcgc acccaccgcc cgattttttc aggagtgaac cccatgagta
    19441 tggttgccag aactaacccc ggccccgcag aggacgacat caccgatacc gatgatggcg
    19501 acactcgcat ttcagcgggt gcattctggc cggatattgt gctgcgtgag ctgcgtctgg
    19561 cggtacgact gccgggccgc gtgaccacct cccgcctgct gcataccgcc accggggcca
    19621 tagcacacgt tacccgcgag ctggaagcat ggcagcagga acaacaggcg gctggctatc
    19681 agacgctggc cgatgttccg gcccctgtaa ttaacggaga aagcgtcaat ctctggcact
    19741 ggcgcaatgc tgtttatacc gccacacgcg ccctgattct ggagcgttac cgcgatgcgg
    19801 acacaacgga caagggcgac cgccgggcgg acgcactgga tatacagaca tcggatttgt
    19861 ggcgcgatgt gagctgggcc atctctgaca ttctgggacg accgcgaatg tttgcggagc
    19921 tgtgctgatg aaagtgaagg cactggaagg cgacaccgtg gattcgctct gtttccggta
    19981 ctacggcacg acacggggcg tcaccgaaaa ggtgctggat gccaaccccg gactctgtca
    20041 gcaggtattt ctggacgccg ggcaggaagt ggagatgccg gagccggaga agaagaaacg
    20101 agaaatgatt cagttgtggg gggagtagca gtgagcacca ttcaaacagg gatcacagag
    20161 caggttattg cgtggctctt tgaccacctg ccaacggtgt atgcagtagg cgcggctgtc
    20221 agcatttccg cgctgatgag tctttatgac ggacgaacac tggttcagac cgtaacggga
    20281 tcgctggcgt gcggcgttct tgccatggcc gtggccgggt cgctgcgctt cttcgggatc
    20341 cctgaggatg cagtgacgtt ttttggtgcc tcaatcggtt ttatgggcgc agagaaagca
    20401 cgcgacaagg ttattgcaat atttgatcgc agggtgaagg agaggaacga atgagcaaca
    20461 catttaaatt cagcagccga agcgaaaaga atttgcaggg tgtaaatcct gatctggtga
    20521 aagtgacccg acgggcgctg gaaatctcgg aagtggattt tggtatcacc gaaggattgc
    20581 gcagtcgtta tcgtcagaag cagctcgtga ccacaggcaa gagccagacc atgaacagtc
    20641 gccatctcac agggcatgcc gtggatgttg tggcttatgt tggcagccag gtgtcatggg
    20701 aatggccgct gtacgaaaaa atcgcagcag cattcagaca ggccagccgg gaactgaata
    20761 ttccggtgga atggggcggc gactggaaga ccctgaaaga cggaccgcat tttcagttac
    20821 cacacggagc ctatccggca tgaagctctg gcccacgctt ggcgtcgctt tccttctgat
    20881 tgccgcatgg ggaacatcca tgcgtctgtc gtggtcgctg ggccgggaga acgccagaaa
    20941 cgaagcgcag gccagcaccc tgaaaagtac cgtcgacaca ctgaatatca tcagcgccgg
    21001 ggtacaggat atgcagcagg tgctggctca actccgcgcg gacaatcagc aacgcaatca
    21061 ggacggagag gtaagacgtg aacagctacg caacgatatt gcaaaagatg aatgcgccca
    21121 cgctttgcct gacgctcgtt ttactgacag gttgcgcagg cacgcagaac gcgccacggc
    21181 cagcgccgtc agtccggctt ataccgcaga cgctgaccat gccggtaacg cctcccccct
    21241 tccctgacca acccacatgg ggaaacctcg gaatatgggg cgaccgcctt ctggatgcac
    21301 tggaaacctg taacgcggat aaacgggcca ttgctgaact ggataagaga atagccgaac
    21361 tgacacacca gacgggagta acacaatgac cagtaagaac tttgcactga ttacagccat
    21421 gacacaggct gaactgactc aaaaggtgaa tgaacatctt gcgaaagggt ggcatcttca
    21481 gggggagacg cgggttgcct acgaacccgg caccccgtgg tatctaatgc aggcaatggt
    21541 ggccgatggc actacagaca tctcacctga ttccccccag cacggcagcg tgccggagtg
    21601 gtattacgtg gtggtacttg ctggtcaatc caatgccatg tcatatggtg agggaatgcc
    21661 gctgccggat tcttacgatg cgccccaccc acgcattaag caactggccc gtcgcaacac
    21721 agtgactccc ggtggtaaag catgcgcatt taacgacatc attccggcag atcactgcct
    21781 gcatgatgtt caggatatga gcgcactgaa tcacccgaat gcagacctga gcaaagggca
    21841 gtatggctgt gtcggacagg gcttgcatat tgccaaacgc ctgttgcctt acattccaca
    21901 gaatgccggg attttactgg ttccatgctg tcgtggtggt tcggcattca cccagggcgc
    21961 ggaggggaca ttcagcgagt ccacaggagc cagtcaggat tcggctcgct ggggtgtggg
    22021 taaaccttta tatcaggacc tgattttgcg cacgaaggcc gcattgcaga aaaacccaaa
    22081 aaacatgctg ctggccgtat gctggatgca gggcgaattt gacatgagcg ccgctacgta
    22141 ctcacagcaa cctccgctgt ttgcggccat gctgaaacag tttcgtgcgg acattaccga
    22201 gtttaacacg cagtgtcatg gaggcagagc ggcaagtgtg ccatggattt gtggtgacac
    22261 gacgtattac tggaaaaaca cctacggcac gcagtacgac accatttacg gggcgtacaa
    22321 aaacagggag agcgacaacg ttttctttgt gccattcctg accgatggta gtggcaacaa
    22381 tacctccacc aacgcaccaa cggaagatcc ggatgctgca agtgagggat attacggttc
    22441 ggcatcccga acgaacaaaa actgggtatc atcaaatcgc cagacgcatt tcagttcatg
    22501 ggcgcgcagg agcattattc cggatcgtat ggcaaccgct attctgaacg tagccggtcg
    22561 cacct
//
LOCUS       DAZGJW010000069        22368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZGJW010000069 DAZGJW010000000
VERSION     DAZGJW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.069"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            101..1711
                     /gene="yeaV"
                     /locus_tag="ACHKIK_002729"
     CDS             101..1711
                     /gene="yeaV"
                     /locus_tag="ACHKIK_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO9083873.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            1743..2867
                     /gene="yeaW"
                     /locus_tag="ACHKIK_002730"
     CDS             1743..2867
                     /gene="yeaW"
                     /locus_tag="ACHKIK_002730"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO9083874.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSIELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            2923..3888
                     /gene="yeaX"
                     /locus_tag="ACHKIK_002731"
     CDS             2923..3888
                     /gene="yeaX"
                     /locus_tag="ACHKIK_002731"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO9083875.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(3942..5057)
                     /gene="rnd"
                     /locus_tag="ACHKIK_002732"
     CDS             complement(3942..5057)
                     /gene="rnd"
                     /locus_tag="ACHKIK_002732"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO9083876.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKVFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPALISGWRGELMAEALHNLLQEYPQ"
     gene            complement(5139..6824)
                     /gene="fadD"
                     /locus_tag="ACHKIK_002733"
     CDS             complement(5139..6824)
                     /gene="fadD"
                     /locus_tag="ACHKIK_002733"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO9083877.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(7029..7610)
                     /gene="yeaY"
                     /locus_tag="ACHKIK_002734"
     CDS             complement(7029..7610)
                     /gene="yeaY"
                     /locus_tag="ACHKIK_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO9083878.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(7650..8345)
                     /gene="tsaB"
                     /locus_tag="ACHKIK_002735"
     CDS             complement(7650..8345)
                     /gene="tsaB"
                     /locus_tag="ACHKIK_002735"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO9083879.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALVYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(8403..10313)
                     /gene="yoaA"
                     /locus_tag="ACHKIK_002736"
     CDS             complement(8403..10313)
                     /gene="yoaA"
                     /locus_tag="ACHKIK_002736"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO9083880.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            10445..10789
                     /gene="yoaB"
                     /locus_tag="ACHKIK_002737"
     CDS             10445..10789
                     /gene="yoaB"
                     /locus_tag="ACHKIK_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO9083881.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            11212..11511
                     /gene="yoaC"
                     /locus_tag="ACHKIK_002738"
     CDS             11212..11511
                     /gene="yoaC"
                     /locus_tag="ACHKIK_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO9083882.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(11631..11810)
                     /gene="yoaH"
                     /locus_tag="ACHKIK_002739"
     CDS             complement(11631..11810)
                     /gene="yoaH"
                     /locus_tag="ACHKIK_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO9083883.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            11884..13245
                     /gene="pabB"
                     /locus_tag="ACHKIK_002740"
     CDS             11884..13245
                     /gene="pabB"
                     /locus_tag="ACHKIK_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO9083884.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            13249..13827
                     /gene="nudL"
                     /locus_tag="ACHKIK_002741"
     CDS             13249..13827
                     /gene="nudL"
                     /locus_tag="ACHKIK_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO9083885.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            14011..15375
                     /gene="sdaA"
                     /locus_tag="ACHKIK_002742"
     CDS             14011..15375
                     /gene="sdaA"
                     /locus_tag="ACHKIK_002742"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO9083886.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            15506..17104
                     /gene="pdeD"
                     /locus_tag="ACHKIK_002743"
     CDS             15506..17104
                     /gene="pdeD"
                     /locus_tag="ACHKIK_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO9083887.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(17108..18664)
                     /gene="yoaE"
                     /locus_tag="ACHKIK_002744"
     CDS             complement(17108..18664)
                     /gene="yoaE"
                     /locus_tag="ACHKIK_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO9083888.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(18652..18810)
                     /gene="yoaL"
                     /locus_tag="ACHKIK_002745"
     CDS             complement(18652..18810)
                     /gene="yoaL"
                     /locus_tag="ACHKIK_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO9083889.1"
                     /translation="MDRHRRHFSIRPFNAYLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            19127..20098
                     /gene="manX"
                     /locus_tag="ACHKIK_002746"
     CDS             19127..20098
                     /gene="manX"
                     /locus_tag="ACHKIK_002746"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO9083890.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAQAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            20161..20961
                     /gene="manY"
                     /locus_tag="ACHKIK_002747"
     CDS             20161..20961
                     /gene="manY"
                     /locus_tag="ACHKIK_002747"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO9083891.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            20974..21825
                     /gene="manZ"
                     /locus_tag="ACHKIK_002748"
     CDS             20974..21825
                     /gene="manZ"
                     /locus_tag="ACHKIK_002748"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO9083892.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            21880..22338
                     /gene="yobD"
                     /locus_tag="ACHKIK_002749"
     CDS             21880..22338
                     /gene="yobD"
                     /locus_tag="ACHKIK_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO9083893.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         5412 a         5820 c         5603 g         5533 t
ORIGIN      
        1 aaataatcaa tttgttaaac acaacataca actcattacc ctgcaaataa ttacaactgg
       61 caaaagccag agacgggctt ttatgcccag gagatgactc atgatgagca atgttaagaa
      121 aaaagatgtg ccgctgataa gcatcagcct ggtggccatt cttttcatcg cagctgcatt
      181 aagccttttc ccacaacaat cggccgacgc ggccaacgca atatacactt ttgttacgcg
      241 tacgttaggt tccgccgtac aggtattggt tttgctggca atgggactgg tgatttattt
      301 agccaccagt aaatacggca atattcgtct tggcgaagga aaaccggaat acagcacgct
      361 ctcctggctg tttatgttta tttgtgccgg tttaggttct tctacgcttt attggggggt
      421 tgctgaatgg gcctattatt atcaaacgcc tggattaaat atcgcaccgc gttcacaaca
      481 ggcactcgaa tttagcgtcc cttactcctt cttccactgg ggcatcagcg cctgggcaac
      541 ttatacgctg gcctcattaa tcatggctta tcactttcat gtgcgtaaaa acaaaggtct
      601 gagtctttcc ggcattattg ccgcgattac cggcgttcgc ccgcaaggcc catggggaaa
      661 actggttgat ttgatgttcc tgatcgccac tgtcggcgca ctgactattt cccttgttgt
      721 caccgcagca acctttactc gtggactttc cgcgctgacc ggtttacccg ataacttcac
      781 cgtgcaggca tttgtgatcc tgctttccgg cggcattttt tgcctaagct cgtggattgg
      841 tatcaacaac ggtttgcaac gtctgagcaa aatggttggc tggggcgcgt tcctgctgcc
      901 attactggtg ctgattgtcg gcccaaccga atttattacc aacagcatca tcaatgccat
      961 cggtctgacc acgcaaaact tcctgcaaat gagcttattc accgatccgc ttggcgatgg
     1021 ttcatttacc cgcaactgga ccgttttcta ctggctgtgg tggatctcat acacccctgg
     1081 cgtagcaatg tttgtcaccc gcgtttcccg cggtcgtaag attaaagaag ttatctgggg
     1141 actgatcctc ggcagcaccg tcggttgctg gttcttcttc ggcgtaatgg aaagctatgc
     1201 cattcatcag tttatcaatg gcgtaatcaa cgtcccacag gtgctggaaa cactgggcgg
     1261 cgaaacagct gtacagcaag ttctgatgtc gttgccagcc ggtaaattgt tcctcgccgc
     1321 atacctgggc gtgatgatta ttttccttgc ctcgcatatg gatgcggtgg cctacaccat
     1381 ggctgcgacc agtacgcgta atctccagga aggtgacgat cctgaccgtg ggctgcgtct
     1441 tttctggtgc gtggtgatca ctctgatccc gctttccatc ttgtttaccg gtgcttcgct
     1501 ggaaacgatg aaaaccaccg tcgtgctcac agcccttccc ttcctcgtca ttttactggt
     1561 gaaagtcggc ggatttattc gctggctgaa acaggattac gccgacattc cggctcatca
     1621 agttgaacat tatctcccgc agacaccggt tgaagccctg gaaaaaacgc cagtgctccc
     1681 tgcgggaacc gtattcaaag gcgacaactg agcgccatca tcctaacgat aaaggtatcc
     1741 ctatgagcaa tctgagccct gactttgtac tacccgaaaa tttttgcgct aacccgcaag
     1801 aggcgtggac cattcctgcc cgtttttata ccgatcagaa cgcgtttgaa cacgaaaaag
     1861 agaacgtctt cgccaaaagc tggatttgcg tcgctcacag cattgaactg gcgaatgcca
     1921 atgattatgt gacgcgtgag atcattggcg aaagcatcgt gctggtacgc ggtcgtgata
     1981 aggttttgcg cgcgttctat aacgtgtgtc cgcaccgtgg tcatcagttg ttgagcggtg
     2041 aaggaaaagc aaaaaatgtg attacctgcc cgtatcacgc atgggcattc aaactcgatg
     2101 gcaatctggc ccatgcacgt aactgcgaaa acgtcgccaa tttcgatagc gacaaagcgc
     2161 aactggttcc ggtgcgtctg gaagaatatg ccggattcgt cttcatcaac atggacccca
     2221 acgccaccag cgtagaagat cagttacccg gtctgggcgc gaaagtgctg gaagcctgcc
     2281 cggaagtcca cgatctgaaa ctggcggccc gctttaccac ccgcacgcct gccaactgga
     2341 agaacattgt cgataactat ctcgagtgct atcactgtgg tccggcgcat ccaggtttct
     2401 ccgactccgt acaggttgat cgttactggc acaccatgca cggtaactgg acgctgcaat
     2461 acggtttcgc caaaccgtcc gaacagtcgt ttaaatttga agagggtacg gatgcggcat
     2521 tccacggttt ctggctgtgg ccgtgcacga tgctgaacgt caccccgatc aaagggatga
     2581 tgacggtcat ttatgaattc ccggtggatt ctgaaactac cctgcaaaac tacgatattt
     2641 acttcaccaa tgaagagtta accgacgagc aaaaatcgct gattgagtgg tatcgcgatg
     2701 tgttccgtcc ggaagattta cgtctggttg aaagcgtaca gaaagggctg aaatcgcgtg
     2761 gctatcgtgg tcaggggcgc atcatggccg acagtagcgg tagtggcatt tccgaacatg
     2821 gtatcgccca tttccataat ctgctggcgc aggtgtttaa ggactaatga catcggcggc
     2881 ggtattttcc gccgctgggc tgatttttga tggagtacag caatgtcaga ctatcaaatg
     2941 tttgaagtac aggtgagtca ggtggaaccg cttaccgaac aggtgaaacg cttcacgctg
     3001 gtggcaaccg atggcaaacc gctgcctgcg ttcaccggag gaagtcacat cattgtgcag
     3061 atgagcgatg gtgataacca gtacagcaat gcgtattcac tactgagttc gccgcatgac
     3121 acctcttgtt atcagattgc cgttcggctg gaggaaaact cgcgcggcgg ttcccgcttt
     3181 ttgcatcagc aggtaaaagt gggcgatcgg ttaacgattt caacgcctaa taacctgttt
     3241 gcgctaattc cctcagccag aaagcatctg tttatcgcgg gcggtattgg tatcacccct
     3301 ttcctgtcgc acatggcaga gctgcaacac agcgacgtcg actggcagct acattactgc
     3361 tcgcgaaatc cagaaagttg cgcatttcgt gatgagctag tccagcatcc gcaggctgag
     3421 aaagttcact tgcatcactc atcaaccgga acacgactgg aattagcgcg attattggcg
     3481 gatatcgaac ctggcacaca cgtttatacc tgtggtcccg aggcgctaaa tgaagcggta
     3541 agaagtgaag ctgcgcgtct ggacatcgtc gccgatacgc tgcactttga gcaatttgct
     3601 atcgaagaca aaaccggcga tgcctttacc ctggtgcttg cccgttccgg aaaagagttt
     3661 gtggtgccgg aagagatgac tattttgcag gttattgaaa ataataaagc cgcgaaagtg
     3721 gaatgtttat gtcgtgaagg ggtatgcgga acctgcgaaa cagcaatact ggaaggtgaa
     3781 gctgaccatc gggatcagta ttttagcgat gaagagcgtg ccagccagca aagtatgttg
     3841 atctgttgtt cgcgtgcgaa gggtaaacgc ctggtgttgg atttgtagtt tgccaaagcc
     3901 ggatgtggcg ctgagcgcgc ccagtccggc ttcggaagat tttactgcgg atattcctgc
     3961 aataaattgt gtaatgcttc cgccatcagc tcaccacgcc agccggaaat cagcgccggc
     4021 aaattgttct gcggtttcag tttccagtgc cagttcagca gttggttgat ttgccgacgc
     4081 gatgccagca attcggcgct gatcttatgc gtttcgctca cgtcagtaat cagcgactta
     4141 atcgctttaa acactttacg ataaccaggc atgtccatca ggttaagcat cggctgcggt
     4201 aaggcctctt ccggcaatgt ctgcgctttt tccaccagcg ccagcagcgt tttaccgtga
     4261 aagcggattt cgctaccgga taaacccagg ctgtccagtt cgcctaaact tcccggcata
     4321 taacgcgcga ccgaccacaa atgctcttca cgcacgacaa agttcaccgc cagatcacgc
     4381 tctcgcgcct tgcgcagtcg ccagtcggct aacagttgca gacaggccag ttggcgtgtg
     4441 cgtaactgcc aggcattggt gatatcacgc caggcatctt ccggcgcaac gacttcctga
     4501 cgacgcattt gcatcaggcg gcattcatcc agcgccgcag gtagccagcc ggaggcctcc
     4561 gtttctacca taagcttggc ggtgatcggt aacagatacc agacatccgc cgctgcgtat
     4621 tcacactgac gttcggtcag cggtctggcc agccagtcgg tgcgcgattc actcttgtcc
     4681 agcgtaacgc cggaatactc ttccaccatg gaagcgaaac cccatgacat cgggcgtccg
     4741 cagaaggcag caaggatttg cgtgtcaatc aagggttgtg gtaattcgcc aaagacattg
     4801 aggaacactt ccagatcttc actgcctgca tggagaaatt ttgtgatgga cggatcgcgc
     4861 aggatcgctt tcagcggtga ccagtcggtg atcccgagag gatcaattag cgccagatgc
     4921 tcgccatcga aaagttgaat caaccccagc tgcgggtaat aagtgcgcgt acgaacaaat
     4981 tcagtatcca gggctatcgc cggaaaggca cggacggctt cacacaaaga agccagcgca
     5041 tcgtccgtgg taatcatttg gtaattcaaa ttgttttctc tttagtgggc gtcaaaaaaa
     5101 acgccggatt aaccggcgtc tgacgactga cttaacactc aggctttatt gtccactttg
     5161 ccgcgcgctt cgtcacgtaa ttctcgtcgc aaaatttttc cgacgttaga tttcggtaac
     5221 tcatcacgaa actccaccag cttcggtact ttgtagcccg tgagctgacg gcggcaaaag
     5281 gtcaccagtg actcttcggt aagcgatgga tcttttttca ctacgaagat tttcaccgct
     5341 tcaccactgg agccggaagg tacgccaaca gccgcgactt cctgtacgcc aggatgctgc
     5401 atgacgacat cttcaatctc gttgggatag acgttaaaac cggaaaccag aatcatgtct
     5461 tttttacgat cgacaatgcg caggaaccct tcttcatcca tcaccgcgat gtcgccggtg
     5521 tgtaaccagc catttttgat gatctcatct gtagcatccg gacgctgcca gtaacccagc
     5581 atcacctgcg gtcctttgac acaaagctca cccggttgac ccggtggtac ttcattatca
     5641 tcatcatcca ccagtttggc ttccgtcgac ggcaccggca aaccgatgct accactatga
     5701 taatcaatat catatgggtt aacgctgacc agcggcgcac actcggtaag gccatagcct
     5761 tccagcagat actgtcctgt cagtttcacc caacgctctg ccaccacttg ctgcactggc
     5821 atccctccgc ctgcggaaag atgcagactg gagaaatcca gctgctggaa ctctttattg
     5881 ttcagcaacg cattgaacaa ggtgttaacg cccgtgatag cggtaaacgg atatttcgct
     5941 aactctttta ccaaccctgg aatatcgcgc gggttagtga taagcaggtt ctgcccaccc
     6001 agttcgataa acagcaggca gttaatggtc agggcaaaaa tgtgatacag cggcagcgcc
     6061 gtcaccacca gctctttgcc cggatgcaac agcggaccat aggtcgcgtt aacctgttcc
     6121 aggttcgcca gcatattgcg gtgagtcagc atcgcgcctt tcgccacacc agtggtgccg
     6181 ccggtgtatt gcagaaaagc taaatcttcc ggcaccagtt cgggtttgac gtactgcatc
     6241 cggtagccgt tatgcagtgc gctacgaaat gaaatggcat ctggcagatg gtatttcggc
     6301 accaaacgct tgatgtattt aacaacgaaa ttgactaccg tgccttttgc cgtagatagc
     6361 tgatcgccca tacgggtcag aattacgtgc tgaacggcgg ttttatcaac cactttttcc
     6421 agtgtgtgag caaagttaga cacgataaca atcgccgatg cgccgctatc gttaagctga
     6481 tgctcaagct cacgcggggt atacaacggg ttaacgttta cgacgatcat cccggcacgc
     6541 aaaatgccaa acagcgccac cggatattgc aataaattag gcatcatcaa cgcaacgcga
     6601 tcgcctttct tcagccccaa cccttgttgc aaataagcgg caaacgcgcg actgcgttct
     6661 tccagcttgc ggaaggtcat tacctccccc atattcacaa acgcaggttg atcggcgtag
     6721 cgcgcgaccg actgctcaaa catatctacc agagattgat aacggtcagg gttgatctcc
     6781 gtcggaacgt ccgcgggata acggttaagc caaaccttct tcaattcttc acctctaaaa
     6841 tgcgtgttcg tcgtcatcgc aaccccaaat gatatacatg ccgttaacat aatattaact
     6901 catcatacca gcttgataat tacccaacga aaaggttgcg aagcgcgtca ctatttattt
     6961 ttatctttac cgtaagaatg cagaaacagc ggaccagccg ctgtttcttt ttctttaaaa
     7021 acaagcaatt attcagttac aactgtttgt actctcgcgg ggccgggatt ataccagccc
     7081 catccgccgt agccataggg ccagccacga ccgccataaa accatggatc aatcggctga
     7141 ggcggcataa tcacctgctg ggttaaatgc caacgtttgt aacccgttac ttgcatcacc
     7201 ataaatttat agggcgtatt gccgattttg ccgtcaaccg caccagtgat tggcccgact
     7261 accgtaacaa gttgtccacg gaaatccacc gggtccagaa aaccgttcac atcggcataa
     7321 atgcgaccgc gagaaggttc tcccagcgtc ggtctggctc cgctgtccag cggtacggta
     7381 gcaatttcca ggcgggtttt cccttgctgg ttttgtaccg caaccacttt gccaccaaag
     7441 cgtgcctcct gaccaacgta cagctgcggc gcactcatca cccgaactaa atcttgttgc
     7501 ggcgtgggac tgctgccttt aatggcgtcc ggcacagtga cacaaccgct cagcattagc
     7561 gcaaacgtac ctgccagtat gcctttgata acattctttt gaaccgccat gatgcgactc
     7621 ctttttttct cagggcatac tcttaagatt cattctttgc ccggaagttt cttccatgcg
     7681 acgttgttac gtaaataaac cggttcggca tgttccaccg ccaccgtttt accctcggca
     7741 aacatctgac acgcaatcgg cagcatatct tccgccgctg gcagtaaaac ttctccgtcg
     7801 cgcaaaacca gcccgctctc tttaccgaga tccggccagg cttgccagcc cgtccctacc
     7861 gttacccatt cgccggaaag ctgttgcatt cgttcatgaa cgagttcggg tttgagtacg
     7921 gcttcggttt cttcaccgcg ccagataccg ttttcatcac gctgatattc ggcccagtaa
     7981 acttcgccca ttcgcgcgtc aatggctgcc agcacgcggg ttgcgccgtt tttgcgccac
     8041 gccccttgcg ccatcgtcat tagcgtggag acgccaatca tcggtagctc tgcgccaagt
     8101 gccagccctt gcgcaatgcc aataccaatg cgcacgccgg taaagctacc ggggccgcgc
     8161 ccgtaaacca gagcgttaat atcagtcagg gaagttccgc tggtggtcag gatatcctgc
     8221 accatcggta agattcgttg agtatgttca cgagggcaaa gctcaaaatg agcgttgaca
     8281 gtaccgtcgt tccacagggc gacagagcag gcctctgtcg cggtatcgat agccagaatt
     8341 cgcatgggtc ttcgtgctta gatcaataaa aaggcgcgca tcataccata ctccgtaaca
     8401 aattacctgg aggatggtat cgcaaggaaa cgaaccgcac gggcaatgtc acgagtgcgt
     8461 ggcgcgggcg gcagactggc gagaaacgtc gcgccgtaag gacgcatcac cagccgattg
     8521 tcacaaatca ccagcacgcc acgatcgtcg gcgtcgcgaa tcagtcgccc taccccctgt
     8581 ttgagagtaa tgacggcatc tggtagttgc acttcatcga acgggtcgcc accgcgcaaa
     8641 cgacaatctt ccatgcgcgc ttttaacagt ggatcatccg gcgaggtaaa cggcaacttg
     8701 tcgataatca ccaatgacaa tgtatcgcca cgcacgtcca ccccttccca gaaactactg
     8761 gttgccacaa gaagcgcgtt accggcgctg acaaattgct gcaacagttg ccctttgctg
     8821 gtttccccct gcaacaaaac gggaagcgtc atggtagcgc ggaactgctc ggccagatca
     8881 cgcatcatgg cgtgcgaggt acaaagcata aaacaacgac cgttgttagc ttcgatgatc
     8941 ggtcgcagca ttgccgctaa ctggcgagca gaacctggct ggttggtttg cggcagattg
     9001 cgcggcacac agagtaacgc ctggcggctg taatcaaacg ggcttggcaa tagcaacgac
     9061 tccgcctgtt ctatcccaag ccgcgaggtg aaatgatgca gatcgtcgtt caccgacagc
     9121 gttgctgagg tgaagatcca gctaccaggc ttttgcgcca ttaactcttt gaatttatcc
     9181 gccacgctga gcggcgtgag agccagagta aaatggcgcg aagtgcattc gtaccagtag
     9241 ctgtagcccg gctgattgat ctcttttagc cgcttcagcc gtgtgcgata caacgtagcg
     9301 cgctcaaatg ccgcatccag caaggcggaa cgccccagtg acagtttcgc cacgtcataa
     9361 caaagttcca gggtgtcatc gagcagtaaa aatgcccgct gaatttgcgg attagctaac
     9421 agctcacgca ggttaccgcg ataacctggc tcaccgagtt gcagacgaaa atcctgcgca
     9481 ctctgggcaa gacgatcagc gcacttttgt aactgctggg tgtcttttaa ttcggtgcgg
     9541 taagcgatgg tgatgtcttt tgccaggtcg agcagttgtc gactggagag tgactgacca
     9601 aaatactggc tggcaatgtc cggtagctgg tgggcttcgt cgaagatcat gacgtccgct
     9661 tccgggatca gttcgccaaa tccactctct ttaaccacca tatccgccag aaagagatga
     9721 tggtttacca ccaccacatc ggcgtccatc gcttttttac gtgctttgac cacaaagcaa
     9781 tctttataca tcgggcagtc gctgccaaga cagttgtcgt tggtgctggt gaccagcggc
     9841 cacgcctgtg aatcttccgc cacgctgacg caggtgctga tatcaccatc gactgtttga
     9901 ttagaccagg agcgcagcag gatcacatcg cttaagattt gtaccggcag atcgcccccc
     9961 gccagcgcct gctgttcgag acgctcgagg cagaggtagt ttgagcgccc tttcagcagc
    10021 gccacgttgc ccgtatattt caatgccttt gagactgttg gcaaatcgcg gctgtagagc
    10081 tgatcctgca acgcttttga gccggtcgag ataatgactt tctttttcgc ccgcagcgca
    10141 ggagccaggt aagcgtaggt tttgcccgta ccggttcctg cttccaccac cagcggctgg
    10201 cctttttcta tcgcctgggt gacggctacc gccatctgtc gctgtggttc tcgcggctta
    10261 aagcctggta tcgctttcgc cagctgaccg tctggtgcaa aatcgtccgt cacactaccc
    10321 cctgttgatt tgaacaggga ttatgtcagg atgagggcgc tttcgccagt tgaattggtg
    10381 acggcgacct cacattgtgg cagtctttgc agcacgaaaa tggaaggata atgaggacaa
    10441 aatgatgact atcgttcgta tcgatgctga agcccgctgg tctgatgtgg taatccacaa
    10501 caacacgctc tactacactg gtgtaccgga aaacctcgac gccgatgcct ttgagcaaac
    10561 cgccaacacg ctggcgcaga ttgatgccgt gctggaaaaa cagggcagca ataaatcgag
    10621 cattctggat gccaccattt tcctggccga taaaaacgac ttcgcggcga tgaataaagc
    10681 gtgggatgcc tgggttgtcg cgggtcatgc gccggtgcgc tgcacggtac aagcgggttt
    10741 gatgaacccg aagtataaag ttgaaattaa gattgtggct gcggtgtaag ctttatcgaa
    10801 gcaaaataag tcagacgata atttatcgat aatactggtc ggttttacat aaatcgacca
    10861 gagattaaga tattacctgt tgatgctcct gctaattcat tatttatttt tataatttca
    10921 attttatcta taaaactaac tatataaata accataacca tgccgttgtg ttcgccctct
    10981 gactagcaac tctggttttc tttcttagct gaataatctg gttatagcgt gaggactgac
    11041 cgttaaattt ctccatctta ctggataaca caccatgagc tgctaccttt aaaacacctg
    11101 aatgtcaaca ggttaactcg tgccatatcg ttataatccc tttgatatta gatgcaaatt
    11161 aaggtcatat atagtcttat cgataacatg gttaatttta aggataagaa tatgcctgct
    11221 gtaatagata aagccctgga tttcattggt gccatggatg tatcagcgcc aacaccaagt
    11281 tcgatgaatg aaagcacggc gaagggaatc tttaaatatt taaaagaact gggagtaccc
    11341 gcgagtgccg ctgatattac ggcgcgagct gaccaggaag gctggaaccc tgggttcacg
    11401 gaaaaaatgg ttggatgggc aaaaaaaatg gagacaggtg aacgctctgt gattaaaaat
    11461 cctgaatact tttcaacata tatgcaggaa gaactaaaag cactggtctg agttaaattt
    11521 atatcagcat aaatgggtca ggacgctttt aatcacatat aaaaagcgtc ctttttctca
    11581 caatcaaatg atcatcggct tctataacga aatcgatacg gattagtgtg ttactcgtct
    11641 tcatcctcaa aacgcgccac gattcgctcg ccggagtggt tggcacgcag ctcttctgcc
    11701 accagcgcga ttgcctggcc gctgctcatc ccttgtgcca tcagttcctg gatccgctcg
    11761 acagcttttt gctgctgttc atgggtgagt gaaggtaaac ctgcaaacat tgttaactcc
    11821 tgctaaattg ttggcgctaa ttatttcatg ctacccggca catagccagt agagtcagga
    11881 ctgatgaaga cgttatctcc cgctgtgatt actttaccct ggcgtcagga cgccgctgaa
    11941 ttttatttct cccgcttaag ccacctgccg tgggcgatgc ttttacactc cggctatgcc
    12001 gatcatccgt atagccgctt tgatattgtg gtcgccgatc cgatttgcac tttaaccact
    12061 ttcggtaaag aaaccgttgt tagtgaaagc gaaaaacgca caacgaccac tgatgacccg
    12121 ctacaggtgc tccagcaggt gctggatcgc gcagacattc gcccaacgca taacgaagat
    12181 ttgccatttc agggcggcgc actggggttg tttggctacg atctgggccg ccgttttgag
    12241 tcactgccag aaattgcgga acaagatatc gttctgccgg atatggcagt gggtatctac
    12301 gattgggcgc tcattgtcga ccaccagcgt catacagttt ctttgctgag tcataatgat
    12361 gtcaatgccc gtcgggcctg gctggaaagc cagcaattct cgccgcagga agatttcacg
    12421 ctcacttccg actggcaatc caatatgacc cgcgagcagt acggcgaaaa atttcgccag
    12481 gtacaggaat atctgcacag cggtgattgc tatcaggtga atctcgccca gcgttttcat
    12541 gcgacctatt ctggcgatga atggcaggca ttccttcagc ttaatcaggc caaccgcgcg
    12601 ccatttagcg cttttttacg tcttgaacag ggtgcaattt taagcctttc gccagagcgg
    12661 tttattcttt gtgataatag tgaaatccag acccgcccga ttaaaggcac gctaccacgc
    12721 ctgcccgatc ctcaggaaga tagcaaacaa gcagaaaaac tggcgaactc agcgaaagat
    12781 cgtgccgaaa atctgatgat tgtcgattta atgcgtaatg atatcggtcg tgttgccgta
    12841 gccggttcgg taaaagtacc agagctcttc gtggtggaac ccttccctgc cgtgcatcat
    12901 ctggtcagca ctataacggc gcgactacca gaacagttac acgccagcga tctgctgcgc
    12961 gcagcttttc ctggtggctc aataaccggg gctccgaaag tacgggctat ggaaattatc
    13021 gacgaactgg aaccgcagcg acgtaatgcc tggtgcggca gcattggcta tttgagcttt
    13081 tgcggcaaca tggataccag cattactatc cgcacgctga ctgccattaa cggacaaata
    13141 tactgctctg cgggcggtgg aattgtcgcc gatagccagg aagaagcgga atatcaggaa
    13201 acttttgata aagttaataa gatattacgc caactggaga agtaagacgt ggaataccgt
    13261 agcctgacgc ttgatgattt tttatctcgc tttcaacttt tgcgcccgca aattaaccgg
    13321 gaaaccctaa atcatcgtca ggctgccgtg ttaatcccca tcgtccgtcg accgcaaccg
    13381 gggttgttgc tgactcagcg ttcaattcat ctgcgtaaac acgctggaca agtggcattc
    13441 cctggaggtg cagtcgatga cacggacgca tcagttatcg ccgccgcgct gcgcgaagct
    13501 gaagaagagg tcgctatacc gccttccgcc gttgaagtta tcggcgtgct gccgcccgtc
    13561 gatagcgtaa ctggctatca ggtaacccca gtggtcggca ttatcccgcc cgatctgccg
    13621 tatcgcgcca gtgaagatga agtctcggcg gtgtttgaaa tgccgctcgc ccaggcatta
    13681 catctgggtc gttatcaccc tttagatatc taccgccgtg gcgattcaca tcgggtatgg
    13741 ctgtcctggt acgaacagta ttttgtatgg ggaatgaccg caggcataat tcgtgagctg
    13801 gcgctgcaaa ttggtgtgaa accctgacta tacttatctt tacatctaca aaacactact
    13861 tgagacaatc atcgcaatat tagttaaatc gcggtttttg attagtttaa ttcatgtgaa
    13921 tagttaagcc agtcgccgcg ttccctctta cactatgcgc tgttattagt tcgttactgg
    13981 aagtccagtc accttgtcag gagtattatc gtgattagtc tattcgacat gtttaaggtg
    14041 gggattggtc cctcatcttc ccataccgta gggcctatga aggcgggtaa acagttcgtc
    14101 gatgatctgg tcgaaaaagg cttactggat agcgttactc gcgttgccgt ggacgtttat
    14161 ggttcactgt cgctgacggg taaaggccac cacaccgata tcgccattat tatgggtctt
    14221 gcaggtaacg aacctgccac cgtggatatc gacagtattc ccggttttat tcgcgacgta
    14281 gaagagcgcg aacgtctgct gctggcacag ggacggcatg aagtggattt cccgcgcgac
    14341 aacgggatgc gttttcataa cggcaacctg ccgctgcatg aaaacggtat gcaaatccac
    14401 gcctataacg gcgatgaagt cgtctacagc aaaacttatt attccatcgg cggcggtttt
    14461 atcgtcgatg aagaacactt tggtcaggat gctgccaacg aagtgagcgt gccgtatccg
    14521 ttcaaatctg ccaccgaact gctcgcgtac tgtaatgaaa ccggctattc gctgtctggt
    14581 ctcgctatgc agaacgaact ggcgctgcac agcaagaaag agatcgacga gtatttcgcg
    14641 catgtctggc aaaccatgca ggcatgtatc gatcgcggga tgaacaccga aggtgtactg
    14701 ccaggcccgc tgcgcgtgcc acgtcgtgcg tctgccctgc gccggatgtt agtttccagc
    14761 gataaactgt ctaacgatcc gatgaatgtc attgactggg taaacatgtt tgcgctggca
    14821 gttaacgaag aaaacgccgc cggtggtcgt gtggtaactg cgccaaccaa cggtgcctgc
    14881 ggtatcgttc cggcagtgct ggcttactat gaccacttta ttgaatcggt cagcccggac
    14941 atctataccc gttactttat ggcagcgggc gcgattggtg cattgtataa aatgaacgcc
    15001 tctatttccg gtgcggaagt tggttgccag ggcgaagtgg gtgttgcctg ttcaatggct
    15061 gctgcgggtc ttgcagaact gctgggcggt agcccggaac aggtttgcgt ggcggcggaa
    15121 attggcatgg aacacaacct tggtttaacc tgcgacccgg ttgcagggca ggttcaggtg
    15181 ccgtgcattg agcgtaatgc cattgcctct gtgaaggcga ttaacgccgc acggatggct
    15241 ctgcgccgca ccagtgcacc gcgcgtctcg ctggataagg tcatcgaaac gatgtacgaa
    15301 accggtaagg acatgaacgc caaataccgc gaaacctcac gcggtggtct ggcaatcaaa
    15361 gtccagtgtg actaatactt cttactcgcc catctgcaac ggatgggcga atttataccc
    15421 gctttctcgt ctgctgtaat attccccact acacttccac tgttgcgtca ggcgtttgtc
    15481 gccatacgct tacagggtgg cccgcatgca aaaagcacaa cggatcatta aaacctatcg
    15541 ccgtaatcga atgattgttt gtacgatttg cgcactcgtt acgctcgctt cgaccctgag
    15601 cgtgcgattt atttcacagc gtaacttaaa tcaacaacgg gtagtacaat tcgccaatca
    15661 cgctgtagag gaattagata aagtactgct tcccctacag gcaggtagcg aagtcttgct
    15721 tccgctgatt ggtctgccct gctctgtcgc ccatttgcca ttacgtaaac aggcggcaaa
    15781 actccaaact gtgcgatcca ttggcctggt gcaagacggc acactctatt gctccagcat
    15841 ttttggttat cgcaatgtgc ccgtcgtgga cattctggct gaacttcctg caccgcaacc
    15901 acttttacgc ctgacgatcg accgtgccct gattaaaggc agtccggttt tgattcaatg
    15961 gacgcctgca gcgggcagta gcaatgctgg ggtcatggag atgattaaca tcgacttact
    16021 gacggcaatg ctccttgagc cacaactgcc gcaaatcagt agcgccagcc tgacggtgga
    16081 caaacggcat ttgctctatg gtaatgggct ggtagattcc cttccgcaac ctgaagacaa
    16141 tgaaaactat caggtttctt cgcaacgctt tccttttacc attaacgtta atggtccggg
    16201 ggctacggcg ctggcatggc actatcttcc aacacaatta ccgctggcgg tgctgctaag
    16261 tttactggtg ggctacatcg cctggctggc gaccgcttac cggatgagct tttcccgcga
    16321 aatcaatctg ggcctggcgc aacatgagtt cgaattgttc tgtcagcctt tgcttaatgc
    16381 gcgcagccag caatgtattg gtgtagagat tttgctgcgc tggaataatc cgcgtcaggg
    16441 ctggatttca ccggatgtgt ttattcctat cgcggaagaa catcatttaa ttgtgccact
    16501 gacccgctat gtgatggcag aaaccattcg tcagcgccat gttttcccga tgagtagtca
    16561 gtttcatgtt ggcattaacg tcgcacccag ccattttcgc cgtggtgtgc tgataaaaga
    16621 tctcaatcag tactggttta gcgctcaccc gattcagcaa ctgatcctcg aaatcaccga
    16681 acgcgatgcc ttactggatg ttgattatcg gattgcccgc gagctacatc gtaaaaacgt
    16741 caaactggcg attgatgact tcggcaccgg caacagttca ttttcctggc ttgaaacatt
    16801 acgtcctgac gtgctgaaaa ttgataagtc atttaccgca gctataggtt ctgacgcggt
    16861 taactcgacg gtgaccgata tcatcatcgc gctggggcaa agactgaata ttgaactggt
    16921 ggcggagggc gtggaaacgc aagaacaggc gaagtatttg cgccgtcatg gcgtgcatat
    16981 tttgcaaggg tatttgtacg cacagccgat gccgctacgt gattttccca aatggctggc
    17041 gggcagccaa ccgccgcccg cccggcataa tggacatatc acgcccatta tgccgttacg
    17101 ttaatgctta ctcatcttca tcgtgcgccg gttgctcttt aacaatgcga accagatcaa
    17161 cacgataatc attggcttca atgatggtga tatgcagtgg ccctacatca atcacatcgc
    17221 ccacacgggg aatgtgacca tttgccgaga tcacgaggcc cgcgaccgtc gcgatatcat
    17281 cgtcatcggc aaggtgctca acatcaagcg cctgctgcaa ggcatgcaaa tctgtaccgc
    17341 cttttaccag ccagccgtca ccatcagtaa tgatttccgg cgtttcgtca gcgtccggga
    17401 attcacccgc aatggcttcc agcacatcca gcggcgtgac cagaccttgt accacaccaa
    17461 actcgttggt cacgataaca aagctcccgc gagcacgacg cagcacgccc aacaggttga
    17521 tcggatcgag ggtttccggg acgataatcg ccggagacgc cgaagcaatc gccgccacat
    17581 caacgccctc ttccagcgcc accagcagtt ctttagcacg tacaataccg atgatttcat
    17641 ccagttcacc gcgacatacc gggaacagac tgtgcggtga agagagcagt tgctcgcgga
    17701 tttcatcgac cccgagatta gcgtcaaccc agcttatttc accgcgcggc gtcatgatcc
    17761 cgcgcagaga acgcgacgcc agcgtcagta cgccgttaat catgtaacgt tcttcttcgg
    17821 caaatgcacc ttccgggatc ggcatcggca tcgggttatc ggcatcgtgc tgaacattgg
    17881 cctgacgttt cccgcccatc aaacgcagga tggcatcggc agtacgcgct cgcagcggca
    17941 aagtcgactg gtggcgaata aagttgcgac gcgcaatctg gttaaacact tcgatgatga
    18001 tcgagaagcc aatcgcggca tacaggtaac ctttcggaat gtggaaaccg aaaccttctg
    18061 ccaccagact cagaccaatc attaacagga agctcagaca gagcaccacc accgtggggt
    18121 gctggttaac gaatcgcgtc agcggtttgg atgccagcaa cataaccgcc atcgcaatca
    18181 ctaccgccgc catcatcacc ggcagatggt taaccatccc tactgcagta attaccgcat
    18241 ccaacgagaa gacggcgtca aggatgacaa tctgtgtgac gaccacccag aaactggcgt
    18301 agcctttacc gtggccggaa tcatgatcgc ggttttccag ccgttcatgc agttcggttg
    18361 ttgctttgaa cagcaagaat atccccccga acaacataat caggtcgcgt ccggagaagg
    18421 agaaatccat gacggtaaat agcggtttgg tcagcgtgac catccatgaa atcagcgaca
    18481 gcagccccag acgcataatc agcgccagtg ataaccccag caaacgcgct ttatcgcgtt
    18541 gttttggcgg cagtttgtca gcaagaatgg cgatgaagac caggttatcg atacccagca
    18601 caatttcgag aacaacaagc gtgagtagcc ccgcccaaat tgaggggtcc attaagaatt
    18661 ccatgacaag ctcctgctta aggaatagct attcgacgcc agaaataatg caggcgtaac
    18721 gacaaaatgc aaacgaaagg tgcggcatag agagccagaa aggtaggcgt taaaaggcct
    18781 gatgctgaaa tgacgtcggt gacgatccat actgcgggct actgccctat actccatggt
    18841 tgttaaacgg gagttaaaca tatcagagac gcctctgatt tggcaaagat ttaccttcct
    18901 ttgcaaacga atgtgacaag gatattttac ctttcgaaat ttctgctaat cgaaagttaa
    18961 attacggatc ttcatcacat aaaataattt tttcgatatc taaaataaat cgcgaaacgc
    19021 aggggttttt ggttgtagcc cttatctgaa tcgattcgat tgtggacgac gattcaaaaa
    19081 tacatctggc acgttgaggt gttaacgata ataaaggagg tagcaagtga ccattgctat
    19141 tgttataggc acacatggtt gggctgcaga gcagttgctt aaaacggcag aaatgctgtt
    19201 aggcgagcag gaaaacgtcg gctggatcga tttcgttcca ggtgaaaatg ccgaaacgct
    19261 gattgaaaag tacaacgctc agttggcaaa actcgacacc actaaaggcg tgctgtttct
    19321 cgttgataca tggggaggca gcccgttcaa tgctgccagc cgcattgtcg tcgacaaaga
    19381 gcattatgaa gtcattgcag gcgttaacat tccaatgctc gtggaaacgt taatggcccg
    19441 tgatgatgac ccaagctttg atgaactggt ggcactggca gtagaaacag gccgtgaagg
    19501 cgtgaaagct ctgaaagcca aaccggttga aaaagccgcg caagcacccg ctgccgcagc
    19561 accaaaagcg gctccaactc cggcaaaacc aatggggcca aacgactaca tggttattgg
    19621 ccttgcgcgt atcgacgacc gtctgattca cggtcaggtc gccacccgct ggaccaaaga
    19681 aaccaatgtc tcccgtatta ttgttgttag tgatgaagtg gctgcggata ccgttcgtaa
    19741 gacactgctc acccaggttg cacctccggg cgtaacagca cacgtagttg atgttgccaa
    19801 aatgattcgc gtctacaaca acccgaaata tgctggcgaa cgcgtaatgc tgttatttac
    19861 caacccaaca gatgtagagc gtctcgttga aggcggcgtg aaaatcacct ctgttaacgt
    19921 cggtggtatg gcattccgtc agggtaaaac ccaggtgaat aacgcggttt cggttgatga
    19981 aaaagatatc gaggcgttca agaaactgaa tgcgcgcggt attgagctgg aagtccgtaa
    20041 ggtttccacc gatccgaaac tgaaaatgat ggatctgatc agcaaaatcg ataagtaacg
    20101 tattgtgttg attatcactc agttttcaca cttaagtctt acgtaaacag gagaagtaca
    20161 atggagatta ccactcttca aattgtgctg gtatttatcg tagcctgtat cgcaggtatg
    20221 ggatcaatcc tcgatgaatt tcagtttcac cgtccgctaa tcgcgtgtac cctggtgggt
    20281 atcgttcttg gggatatgaa aaccggtatt attatcggtg gtacgctgga aatgatcgcg
    20341 ctgggctgga tgaacatcgg tgctgcagtt gcgcctgacg ccgctctggc ttctatcatt
    20401 tctaccattc tggttatcgc aggtcatcag agcattggtg caggtatcgc actggcaatc
    20461 cctctggccg ctgcgggcca ggtactgacc atcatcgttc gtactattac cgttgctttc
    20521 cagcacgctg cggataaggc tgctgataac ggcaacctga cagcgatttc ctggatccac
    20581 gtttcttctc tgttcctgca agcaatgcgt gtggctattc cggccgtcat cgttgcgctg
    20641 tctgttggta ccagcgaagt acagaacatg ctgaatgcga ttccggaagt ggtgaccaat
    20701 ggtctgaata tcgccggtgg catgatcgtg gtggttggtt atgcgatggt tatcaacatg
    20761 atgcgtgctg gctacctgat gccgttcttc tacctcggct tcgtaaccgc agcattcacc
    20821 aactttaacc tggttgctct gggtgtgatt ggtactgtta tggcagtgct ctacatccaa
    20881 cttagcccga aatacaaccg cgtagccggt gcgcctgctc aggcagctgg taacaacgat
    20941 ctcgataacg aactggacta acaggtgagc gaaatggttg atacaactca aactaccacc
    21001 gagaaaaaac tcactcaaag tgatattcgt ggcgtcttcc tgcgttctaa cctcttccag
    21061 ggttcatgga acttcgaacg tatgcaggca ctgggtttct gcttctctat ggtaccggca
    21121 attcgtcgcc tctaccctga gaacaacgaa gctcgtaaac aagctattcg ccgtcacctg
    21181 gagttcttta acacccagcc gttcgtggct gcgccgattc tcggcgtaac cctggcgctg
    21241 gaagaacagc gtgctaatgg cgcagagatc gacgacggtg ctatcaacgg tatcaaagtc
    21301 ggtttgatgg ggccgctggc tggtgtaggc gacccgatct tctggggaac cgtacgtccg
    21361 gtatttgcag cactgggtgc cggtatcgcg atgagcggca gcctgttagg tccgctgctg
    21421 ttcttcatcc tgtttaacct ggtgcgtctg gcaacccgtt actacggcgt agcgtatggt
    21481 tactccaaag gtatcgatat cgttaaagat atgggtggtg gcttcctgca aaaactgacg
    21541 gaaggggcgt ctatcctcgg cctgtttgtc atgggggcat tggttaacaa gtggacacat
    21601 gtcaacatcc cgctggttgt ctctcgcatt actgaccaga cgggcaaaga acacgttact
    21661 actgtccaga ctattctgga ccagttaatg ccaggcctgg taccactgct gctgaccttt
    21721 gcttgtatgt ggctactgcg caaaaaagtt aacccgctgt ggatcatcgt tggcttcttc
    21781 gtcatcggta tcgctggtta cgcttgcggc ctgctgggac tgtaagactg ttgtacacta
    21841 ccggggcctt ttggccccgt ttttttatct ggaggattaa tgacaatcac ggacctggta
    21901 ctgattcttt tcatcgcagc actcctggcc ttcgcgatct acgatcagtt catcatgccc
    21961 cgccgtaacg gccccaccct gctggcaatt cctttgctcc ggcgtggtcg catcgatagc
    22021 gttatcttcg tcggattgat tgtcattctt atctataaca acgtcacgaa tcatggtgcg
    22081 ttaataacga catggttatt aagcgcactg gctctgatgg gtttttatat attctggatc
    22141 cgcgttccga agatcatctt taaacaaaaa ggttttttct tcgccaatgt ctggattgaa
    22201 tatagccgaa tcaaagcgat gaacttgtcg gaagatggcg tgttggtgat gcaattagaa
    22261 cagcgtcggc tgttaatccg cgttcgaaat atcgacgatc tggaaaaaat ttataagctt
    22321 ctcgtttcaa ctcaataagt tatgaattta gccaaagcta tgtttagt
//
LOCUS       DAZGJW010000070        22351 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZGJW010000070 DAZGJW010000000
VERSION     DAZGJW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.070"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(38..199)
                     /gene="rsmS"
                     /locus_tag="ACHKIK_002750"
     CDS             complement(38..199)
                     /gene="rsmS"
                     /locus_tag="ACHKIK_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO9083894.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(213..740)
                     /gene="priC"
                     /locus_tag="ACHKIK_002751"
     CDS             complement(213..740)
                     /gene="priC"
                     /locus_tag="ACHKIK_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO9083895.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            810..1187
                     /gene="ybaN"
                     /locus_tag="ACHKIK_002752"
     CDS             810..1187
                     /gene="ybaN"
                     /locus_tag="ACHKIK_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO9083896.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            1340..1891
                     /gene="apt"
                     /locus_tag="ACHKIK_002753"
     CDS             1340..1891
                     /gene="apt"
                     /locus_tag="ACHKIK_002753"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO9083897.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            2020..3951
                     /gene="dnaX"
                     /locus_tag="ACHKIK_002754"
     CDS             2020..3951
                     /gene="dnaX"
                     /locus_tag="ACHKIK_002754"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO9083898.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    3284..3348
                     /gene="dnaX"
                     /locus_tag="ACHKIK_002754"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            4004..4333
                     /gene="ybaB"
                     /locus_tag="ACHKIK_002755"
     CDS             4004..4333
                     /gene="ybaB"
                     /locus_tag="ACHKIK_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO9083899.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            4333..4938
                     /gene="recR"
                     /locus_tag="ACHKIK_002756"
     CDS             4333..4938
                     /gene="recR"
                     /locus_tag="ACHKIK_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO9083900.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            5048..6922
                     /gene="htpG"
                     /locus_tag="ACHKIK_002757"
     CDS             5048..6922
                     /gene="htpG"
                     /locus_tag="ACHKIK_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO9083901.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVELEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDADKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            7103..7747
                     /gene="adk"
                     /locus_tag="ACHKIK_002758"
     CDS             7103..7747
                     /gene="adk"
                     /locus_tag="ACHKIK_002758"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO9083902.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            7983..8945
                     /gene="hemH"
                     /locus_tag="ACHKIK_002759"
     CDS             7983..8945
                     /gene="hemH"
                     /locus_tag="ACHKIK_002759"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO9083903.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKMYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(8942..9901)
                     /gene="aes"
                     /locus_tag="ACHKIK_002760"
     CDS             complement(8942..9901)
                     /gene="aes"
                     /locus_tag="ACHKIK_002760"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO9083904.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            10053..11357
                     /gene="gsk"
                     /locus_tag="ACHKIK_002761"
     CDS             10053..11357
                     /gene="gsk"
                     /locus_tag="ACHKIK_002761"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO9083905.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(11490..13166)
                     /gene="ybaL"
                     /locus_tag="ACHKIK_002762"
     CDS             complement(11490..13166)
                     /gene="ybaL"
                     /locus_tag="ACHKIK_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO9083906.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(13404..14624)
                     /gene="fsr"
                     /locus_tag="ACHKIK_002763"
     CDS             complement(13404..14624)
                     /gene="fsr"
                     /locus_tag="ACHKIK_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO9083907.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            14842..16494
                     /gene="ushA"
                     /locus_tag="ACHKIK_002764"
     CDS             14842..16494
                     /gene="ushA"
                     /locus_tag="ACHKIK_002764"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO9083908.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGKMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGRLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(16531..17010)
                     /gene="ybaK"
                     /locus_tag="ACHKIK_002765"
     CDS             complement(16531..17010)
                     /gene="ybaK"
                     /locus_tag="ACHKIK_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO9083909.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(17214..18008)
                     /gene="ybaP"
                     /locus_tag="ACHKIK_002766"
     CDS             complement(17214..18008)
                     /gene="ybaP"
                     /locus_tag="ACHKIK_002766"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO9083910.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            18146..18487
                     /gene="ybaQ"
                     /locus_tag="ACHKIK_002767"
     CDS             18146..18487
                     /gene="ybaQ"
                     /locus_tag="ACHKIK_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO9083911.1"
                     /translation="MKQATRKPTTPGDILLYEYLVPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(18601..21105)
                     /gene="copA"
                     /locus_tag="ACHKIK_002768"
     CDS             complement(18601..21105)
                     /gene="copA"
                     /locus_tag="ACHKIK_002768"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO9083912.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            21367..22299
                     /gene="glsA"
                     /locus_tag="ACHKIK_002769"
     CDS             21367..22299
                     /gene="glsA"
                     /locus_tag="ACHKIK_002769"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO9083913.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
BASE COUNT         5496 a         5971 c         5905 g         4979 t
ORIGIN      
        1 acaatcttag gggacattta gcggccccat ttatttctca cttttccgcc tcatcatcgc
       61 gcgttaattt cttttcataa tcgcgtttta caatatccag cgcgcgcagc acggtgcggg
      121 ctgggatctg attttcttcc agcagcacaa tcaaatcgac agccagtttg acatcgtcag
      181 gggcattttc cagtgacata ctctctccat tgctagcggg ttaagcgcgc tagcctgttt
      241 tcgatttttt ccagcgcatg gcggcagcgt gccaggcgcg cttcataggc ttccacttca
      301 cgatgcagcg tttgctgttc cacgagatcg gtcacccgcg ccaggcgggc tctgcgttcg
      361 gcaaccatct cacgtagccg ccgctcaaaa tcctgatgct gaatacgttt acgctgccag
      421 cgggcaattt tcggtggcgc actgtcccac tcgcgcaatg accaggcgga ggcttcacgc
      481 gcgatggctt ccagttgtgc cgccagatgt tccgccagcc aggccacttg cggcagctgt
      541 tgctgctcaa ctgcatgacg aagcgcagcc agattatcgc ccgcctcatc gagacaagcc
      601 tgcagtgttg tcgcacgggt ttgaaaaagg tgtctgtcaa aacgggcact tagcgtggca
      661 aattgcgcta ccggggcaca acgctgacgc agcgtagcga gctgtccttc cagtttttcc
      721 agcagcaggg cggttttcac gataagacct caatgaaaat gataattgtt atgctaaagt
      781 agccactctg taagctgaca acactcaata tgcaacgaat cattttaatc atcattggct
      841 ggctggcggt agtgctgggt acgctgggcg tggtattacc ggtattaccg acgacgccgt
      901 ttatcctgct ggcggcctgg tgctttgccc gttcttcccc gcgctttcac gcctggttgc
      961 tgtaccgctc atggtttggc agctatctac gtttctggca gaaacatcat gcgatgccgc
     1021 gcggcgtcaa accgcgggcg attttgctta ttttgctcac gtttgccatt tctctgtggt
     1081 tcgtccagat gccatgggtg cgcatcatgt tgctggtaat tctcgcctgt ttgcttttct
     1141 atatgtggcg aattccggtg attgatgaaa agcaagaaaa gcactgaagc acaacaatcg
     1201 cagttgcaat tattgcgtac agccagtaca ttctggcgtt ttcgagcaca ggcgcaggcg
     1261 gtcaaaggtt aaacaactgt tacttttgat acgtttaaaa cgcgccgtga gtaccaccgt
     1321 aacaagcagg catacactta tgaccgcgac tgcacagcag cttgagtatc tcaaaaatag
     1381 catcaaaagc attcaggact acccaaaacc cggcattctt ttccgcgatg tcaccagctt
     1441 actggaagac ccgaaagctt acgctctcag catcgacttg ctggttgagc gttacaaaaa
     1501 tgcgggcatt accaaagttg tcggcaccga agcgcgtggc ttcttgtttg gcgctccggt
     1561 agctctgggt ctgggcgttg gctttgtacc ggtccgtaaa ccgggcaaac tgccgcgtga
     1621 aaccatcagt gaaacttacg acctggaata cggcaccgat cagctggaga tccacgttga
     1681 tgccatcaaa ccgggcgaca aagttctggt ggtggacgat ctgctggcaa ccggcggcac
     1741 tatcgaagcg accgttaaac tgatccgtcg tctgggtggt gaagtggctg acgctgcgtt
     1801 cattatcaac ctgttcgatc tcggcggcga acagcgtctc gaaaaacagg gcattaccag
     1861 ctacagcctt gtcccgttcc cgggccatta attatcgcca gtcttgtgct gcccacgcta
     1921 cggacagcac aagatgtgca ttcagcctcg ccgttctgac ggggctgtgt tagcattacc
     1981 ccttcgtgaa tccaccttcc agcgtttcag agcctgccaa tgagttatca ggtcttagcc
     2041 cgaaaatggc gcccacaaac ctttgctgac gtcgtcggcc aggaacatgt gctgaccgca
     2101 ctggcgaacg gcttgtcgtt agggcgtatt catcatgctt atcttttttc cggtacccgt
     2161 ggcgtcggaa aaacctctat cgcccgactg ctggcgaagg ggctaaactg cgaaaccggc
     2221 attaccgcga cgccgtgcgg cgtgtgcgat aactgtcgtg aaatcgagca ggggcgcttt
     2281 gtcgatctga ttgaaatcga cgccgcctcg cgcaccaaag ttgaagatac ccgcgacctg
     2341 ctggataacg tccagtacgc tccggcgcgt ggtcgtttca aagtttatct gatcgacgaa
     2401 gtgcatatgc tgtcgcgcca cagctttaac gcactgttaa aaacccttga agagccgccg
     2461 gagcacgtta agtttctgct ggcgacgacc gatccacaga aattgccggt gacgattttg
     2521 tcgcgctgtt tgcaatttca tctcaaggcg ctggatgtcg agcaaattcg ccatcaactt
     2581 gagcacatcc tcaacgaaga acatatcgct cacgagccgc gggcgttgca attgctggcg
     2641 cgcgccgctg aaggcagcct gcgagatgcc ttaagtctga ccgaccaggc gattgccagc
     2701 ggtgacggcc aggtttcaac ccaggcagtc agtgcgatgc tgggtacgct tgacgacgat
     2761 caggcgctgt cgctggttga agcgatggtc gaggccaacg gcgagcgcgt aatggcgctg
     2821 attaatgaag ccgctgcccg tggtatcgag tgggaagcgt tgctggtgga aatgctcggc
     2881 ctgttgcatc gtattgcgat ggtacaactt tcgcctgctg cacttggcaa cgacatggcc
     2941 gccattgagc tgcggatgcg tgaactggcg cgcaccatac cgccgacgga tattcagctt
     3001 tactatcaga cgctgttgat tggtcgcaaa gaattaccgt atgcgccgga ccgccgcatg
     3061 ggcgttgaga tgacgctgct gcgcgcgctg gcgttccatc cacgtatgcc tctgcctgag
     3121 ccagaagtgc cacgccagtc ctttgcgccc gtcgcgccaa cggcagtaat gacgccaacc
     3181 caggtgccgc cgcaaccgca atcagcgccg cagcaggcac cgactgtacc gctcccggaa
     3241 accaccagcc aggtgctggc ggcgcgccag cagttgcagc gcgtgcaggg agcaaccaaa
     3301 gcaaaaaaga gtgaaccggc agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg
     3361 gaaagactgg cttcggtcac cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa
     3421 aaagcgccag ccaaaaaaga agcgtatcgc tggaaggcta ccactccagt gatgcagcaa
     3481 aaagaagtgg tcgccacgcc gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg
     3541 gaactggcgg cgaagctggc ggcagaagcc attgagcgcg acgcgtgggc ggcacaggtg
     3601 agtcaacttt cgctaccaaa actggtcgaa caggtggcct taaatgcctg gaaagaggag
     3661 agcgacaacg cagtatgtct gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt
     3721 gcacagcaaa aactggctga agcgttgagc acgttaaaag gttcaacggt tgaactgact
     3781 atcgttgaag atgataatcc cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac
     3841 gaagaaaaac ttgcgcaggc gcgcgagtcc attattgcgg ataataatat tcagaccctg
     3901 cgtcggttct tcgatgcgga gctggatgaa gaaagtatcc gccccatttg atcgtaagca
     3961 cagcttacgt tcgtcatcct taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg
     4021 tctgggtaac ctgatgaagc aagcccagca gatgcaagaa aaaatgcaga aaatgcagga
     4081 agagatcgcg cagctggaag tcaccggcga atctggcgca ggtctggtaa aagtgaccat
     4141 caacggtgca cacaactgcc gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa
     4201 agagatgctg gaagacctgg tggctgcagc attcaacgac gcagcacgtc gtattgaaga
     4261 aacgcagaaa gaaaaaatgg cctctgtatc ctccggaatg cagctgccgc ctggctttaa
     4321 gatgccgttc tgatgcaaac cagcccgctg ttaacacagc ttatggaagc actgcgctgt
     4381 ctgccgggcg ttggcccgaa gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat
     4441 cgtagcggcg ggatgcgtct ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac
     4501 tgcgccgatt gccgcacttt caccgaacag gaagtctgta acatctgttc gaatccgcgt
     4561 cgtcaggaaa acggtcaaat ctgtgtggtg gagagtccgg cggacatcta cgccattgag
     4621 cagacggggc agttttcagg ccgttatttt gtgttgatgg ggcacctgtc accgctggac
     4681 ggcatcggtc cggatgatat cggccttgat cgtctggaac agcgtctggc agaggaaaaa
     4741 atcactgaag tgatcctcgc caccaatccc acggttgaag gtgaagctac cgctaactac
     4801 attgccgagc tttgcgcgca atatgacgtg gaagccagcc gaatcgctca tggcgtaccg
     4861 gttggcggcg aactggaaat ggtcgatggc accacgctgt cacactccct tgccgggcgt
     4921 cataagattc gtttttaagc aaacgagagc aggatcacct gctctcgctt gaaattattc
     4981 tcccttgtcc ccatctctcc ctcatcctgt ttttaacctt aaaatggcat tattgaggta
     5041 gacctacatg aaaggacaag aaactcgtgg ttttcagtca gaagtgaaac agcttctgca
     5101 cctgatgatc cattctctct attccaataa agaaatcttc ctgcgtgagc ttatctctaa
     5161 cgcctccgat gcggcggaca agctgcgttt ccgtgcgcta tctaacccgg acctgtacga
     5221 aggtgatggc gaactgcgcg ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat
     5281 ctccgataac ggcgtgggga tgacccgcga cgaagtgatt gaccatctgg ggactatcgc
     5341 taaatccggt accaaatcat tcctcgaatc cctgggttct gaccaggcga aagacagcca
     5401 gctgatcggt cagtttggtg ttggtttcta ctctgcgttt atcgtggccg acaaagtgac
     5461 cgtgcgtact cgcgcggcag gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg
     5521 cgaaggtgaa tataccgttg ccgacatcac caaagaagat cgtggtactg aaatcaccct
     5581 gcatctgcgt gaaggcgaag acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag
     5641 caaatactcc gaccatatcg cgctgccggt agagctcgaa aaacgcgaag agaaagacgg
     5701 cgaaaccgtt atctcctggg agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa
     5761 gtcggaaatc accgatgaag agtacaaaga gttctacaaa cacatcgccc acgactttaa
     5821 tgatccgctg acctggagcc acaaccgcgt tgaaggtaag caggagtaca ccagcctgct
     5881 gtacatcccg tcccaggctc cgtgggatat gtggaaccgt gatcataaac acggcctgaa
     5941 actgtacgtc cagcgtgtgt tcatcatgga cgacgcagaa cagttcatgc cgaactatct
     6001 gcgcttcgtg cgtggtctga ttgactccag cgatctgccg ctgaacgttt cccgtgaaat
     6061 cctccaggac agcacggtaa cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca
     6121 aatgctggaa aaactggcga aagacgacgc ggacaaatac cagaccttct ggcaacagtt
     6181 tggcctggta ctgaaagaag gtccggcgga agatttcgct aaccaggaag cgatcgccaa
     6241 actgctgcgt tttgcttcta cttataccga ttcttctgcg cagaccgtgt ctctggaaga
     6301 ctacgtttcc cgcatgaaag aagggcagga gaaaatctac tacatcactg cagacagcta
     6361 tgcggcagcg aagagcagcc cgcacctgga actgctgcgt aagaaaggca tcgaagttct
     6421 gctgctttcc gaccgcatcg atgagtggat gatgaactat ctgactgagt tcgacggtaa
     6481 accgttccag tcggtgtcta aagttgacga gtcgctggaa aaactggctg acgaagttga
     6541 tgagagcgcg aaagaagcgg agaaagcact gactccgttc atcgaccgtg tgaaagccct
     6601 gctcggcgag cgcgtgaaag atgtccgtct gactcaccgt ctgaccgata cgccagcgat
     6661 cgtttcgacc gacgcggacg aaatgagcac tcagatggcg aaactgttcg ctgcggcggg
     6721 ccagaaagtg ccagaagtga aatacatctt cgaactgaac ccggatcacg tactggtgaa
     6781 acgtgcggca gatactgaag atgaagcgaa gttcagcgag tgggtagaac tgctgctgga
     6841 tcaggcgctg ctggcagaac gcggcacgct ggaagatccg aacctgttta ttcgtcgtat
     6901 gaaccagctg ctggtttcct gatgtcatgc cggatgacct ttgtgtcatc cggcattttt
     6961 cttttcatca tctgcacttt ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg
     7021 cctttcttga ggcaatcgcc tgttggtggt atcgtttatc gctttttcaa aaaattcgac
     7081 acattttaag gggattttcg caatgcgtat cattctgctt ggcgctccgg gcgcggggaa
     7141 agggactcag gctcagttca tcatggagaa atatggtatt ccgcaaatct ccactggcga
     7201 tatgctgcgt gctgcggtca aatctggctc cgagctgggt aaacaagcaa aagacattat
     7261 ggatgctggc aaattggtca ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc
     7321 tcaggaagac tgccgtaatg gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc
     7381 agacgcgatg aaagaagcgg gcatcaatgt tgattacgtt ctggaattcg acgtaccgga
     7441 cgaactgatt gttgatcgta tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta
     7501 tcacgttaaa ttcaatccgc cgaaagtaga aggcaaagac gacgttaccg gtgaagaact
     7561 gactacccgt aaagacgatc aggaagaaac cgtgcgtaaa cgtctggttg aataccatca
     7621 gatgactgca ccgctgatcg gctactactc caaagaagcg gaagcgggta acaccaaata
     7681 cgcgaaagtt gacggcacca agccggttgc tgaagttcgc gctgatctgg aaaaaatcct
     7741 cggctaattc gaaagcgcgc acggacagtc ccctcgcccc ctcggggaga gggttagggt
     7801 gaggggaaca ggcccgcaca agcaaactta tcagcaatct caggccggat attcattcgg
     7861 ccttttacaa aaacctcaat ctgcgctatt cctttttctg atttgacctc tcacaacaat
     7921 tagttcttct tcctcacttt tccgctacaa ttatcaacaa gttgaatcga taagaggcgg
     7981 taatgcgtca gactaaaacc ggtatcctgc tggcaaacct gggtacgccc gatgccccca
     8041 cacctgaagc ggtaaaacgc tatctgaaac aatttttaag cgacagacgc gtggttgata
     8101 cctcacggtt gttatggtgg ccgttgctgc gcggcgtgat tttgccgctg cgctcgccgc
     8161 gtgtggcgaa gatgtacgcc tcggtctgga tggaagacgg ctcgccgctg atggtttaca
     8221 gccgccagca acagcaggcg ttggcacaac gtttaccgga tacgcccgta gcgctgggca
     8281 tgagctacgg ctcgccatca ctggaaagcg ccgtcgatga actcctggca gagcatgtag
     8341 atcatattgt ggtgctgccg ctttatccgc aattctcctg ttctacggtc ggtgcggtat
     8401 gggatgaact ggcacgcatt ctggcgcgca aacgtagcat tccggggata tcgtttattc
     8461 gtgattacgc cgataaccac gattacatta atgcactggc gaacagcgta cgcgcttctt
     8521 ttgccaaaca tggcgaaccg gatctgctgc tgctctctta tcatggcatt ccccagcgtt
     8581 atgcagatga aggcgatgat tacccgcaac gttgccgcac aacgactcgc gaactggctt
     8641 ccgcactggg gatggcaccg gaaaaagtga tgatgacctt tcagtcgcgc tttggccggg
     8701 aaccctggct gatgccttat accgacgaaa cgctgaaaat gctcggagaa aaaggcgtag
     8761 gtcatataca ggtgatgtgc ccgggctttg ctgcggattg tctggagacg ctggaagaga
     8821 ttgccgagca aaaccgtgag gtcttcctcg gtgccggcgg gaaaaaatat gaatatattc
     8881 cagcgcttaa tgccacgccg gaacatatcg aaatgatggc taatcttgtt gccgcgtatc
     8941 gctaaagctg agcggtaaag aactgagcgc cgtcgcgaag agcctcgtcg gcggttttca
     9001 tcattcgtga ataatgcaaa aaggcatgca gcgtacctgg gtagagtttg aactcacagg
     9061 gctgctgatg cgccgctaac gtctggtaaa gcagacggct gtcatccagc agcggatcga
     9121 actccgcccc ggcaataaaa cagggcggaa cttcgcgagt gagatcatta ttaaacagac
     9181 agtaatacgg tgactcgcgg tccgcgtcgt tgcttaaata cgcctcttca tacatctgca
     9241 aatcctgttg cgttaagcca tcccagacac cgcccaacag acgacgagtc acggaatccc
     9301 gtaatccgta aagcccatac cacagcaaaa cacccgcaat tttaccgcaa tcgatctgtt
     9361 tatcacgcaa ccacaacgca ctggcgagcg ccagcatggc acctgcggaa tcaccggcaa
     9421 agccaatgcg ggacatattg atttgataat cctccgcctg ctggtggaaa taacaacaag
     9481 cagccacaat ttcctctatc gcttgcggaa aacgcgcttc aggtgaaagg gggtaatcaa
     9541 taccaatcac cgtacattgg ctgtagcttg ccagcaggcg catgatgcga tcgtgggtat
     9601 cgagattgcc gagaataaaa ccgcctccat gcaaataaaa tagcgtcgcc gggctatctg
     9661 gctgcggaca aaagagacgt gtttccacct gcccatattt tgttggaacc atgtaagctc
     9721 tggttgccat ttctggagcg cccgcattcc agaatcggcg ctcaagcgtg taatactgtc
     9781 gttgctcagc aatcgttccc gttgcgggcc aggacggtaa atccggctga agagtattca
     9841 caacggtctt catttcagca gaaataaggt ccagaacagg tagtttgttt tccggcttca
     9901 tacaaaaact cctttcaaat tacgtcattg taaggaacca ctgccatgaa aatgcgatcc
     9961 cgcctgctga tattgaaact ggctgcgtct cgcgcgctcc cgtcagattg tgttaacatt
    10021 cgccgctcag ttaaccaccc gtaaaaacaa ccatgaaatt tcccggtaaa cgtaaatcca
    10081 aacattactt ccctgtaaat gcacgcgatc cgctgcttca gcaattccag ccagaaaacg
    10141 aaaccagcgc cgcctgggtc gtaggtattg accagacgct ggtcgatatt gaagcgaaag
    10201 tggatgatga attcattgag cgttatggat taagcgccgg gcattcactg gtgattgagg
    10261 atgacgtagc cgaagcgctt tatcaggagc taaaacagaa aaacctgatt acccatcagt
    10321 ttgcgggtgg caccattggt aacaccatgc acaactactc ggtgctcgcg gacgaccgtt
    10381 cggtgctgct gggcgtcatg tgcagcaata ttgaaattgg cagttatgcc tatcgttacc
    10441 tgtgtaacac ttccagccgt accgatctta actatctaca aggcgtggat ggcccgattg
    10501 gtcgttgctt tacgctgatt ggcgagtccg gggaacgtac ctttgctatc agtccaggcc
    10561 acatgaacca gctgcgggct gaaagcattc cggaagatgt gattgccgga gcctcggcac
    10621 tggttctcac ctcatatctg gtgcgttgca agccgggtga acccatgccg gaagcaacca
    10681 tgaaagccat tgagtacgcg aagaaatata acgtaccggt ggtgctgacg ctgggcacca
    10741 agtttgtcat tgccgagaat ccgcagtggt ggcagcaatt cctcaaagac cacgtttcta
    10801 tccttgcgat gaacgaagat gaagccgaag cgttgaccgg agaaagcgat ccgttgttgg
    10861 catctgacaa ggcgctggac tgggtagatc tggtgctgtg caccgccggg ccaatcggct
    10921 tgtatatggc gggctttacc gaagacgaag cgaaacgtaa aacccagcat ccgctgctgc
    10981 cgggcgctat agcggaattc aaccagtatg agtttagccg cgccatgcgc cacaaggatt
    11041 gccagaatcc gctgcgtgta tattcgcaca ttgcgccgta catgggcggg ccggaaaaaa
    11101 tcatgaacac taatggagcg ggggatggcg cattggcagc gttgctgcat gacattaccg
    11161 ccaacagcta ccatcgtagc aacgtaccaa actccagcaa acataaattc acctggttaa
    11221 cttattcatc gttagcgcag gtgtgtaaat atgctaaccg tgtgagctat caggtactga
    11281 accagcattc acctcgttta acgcgcggct tgccggagcg tgaagacagc ctggaagagt
    11341 cttactggga tcgttaagtt atcgtcggtt cgtaggccag ataaggcgtt cacgccgcat
    11401 ctggcatttg gctctcgatg cctgatgcga cgctggcgcg tcttatcatg cctacatatt
    11461 tttcatattt tacatccggc aaccaccgtt taccccgtca ccacctcacc cgccggtggc
    11521 gtttccagca gttccagcat ggtacgggcg atttcacgct cgcccatcac tacctgattc
    11581 gcaccacgtt cggtgatata cgccacttca tcgtcataat gggcgcgggc aataatctca
    11641 atatccggat ttttcgcgcg ggcagatgcc acaatctcac ccgcttcata accgttggga
    11701 atcgtcagga tcagccattt tgcacattcc agatgcgcca gttgcataat ttcttcgttc
    11761 gccgcattgc ccaatactgc gcggaccccg cgctctcgca gctcatcaac acgggttcgt
    11821 gacgtctcaa tcaccaccag cggaatatca gaggcgagca atttctcccc cagcaggctg
    11881 cctacacgac cgtaacccac cagtagcgca tggttgcaaa tatccactgg gatctgcttc
    11941 tcttcttcga ttgcctcttc cagcgtctgc tcttccagcg tttcggtctt cgccagatat
    12001 ttctccagta gtgcgaacag taccgggttg agcataatcg acaggatcgc ccctgccagt
    12061 actaggtttt gtccggcctg cggcagtaaa ttcaatgcca ttcccagtcc cgccaggata
    12121 aacgcgaact caccaatctg cgccaggctg gcggcgatgg ttaatgccgt acgttgggag
    12181 tgaccaaaca gtcgcaccag gaaaaatgcg gctaacgact taccaaacag aataatcgcc
    12241 agcgtcgcca gcactgccag cggttgctga atcagaatta acggatcaaa caacatcccg
    12301 acggagacaa aaaacagcac cgcaaacgcg tcgcgcaatg gcagcgtatc gtgggcggca
    12361 cggtgactca gttcagactc gttcagtacc atcccggcaa agaacgcacc gagtgcaaag
    12421 gagacatcaa acagctctac cgcaccaaag gcaatcccta acgccagcgc cagcaccgac
    12481 agggtaaaca gctcgcgaga accggttgcc gcgctgcgtg ccataatcca cggcaccaga
    12541 cggcgaccta ccagcatcat aatggcgata aatgcgatca ctttgccgat ggtgatcccc
    12601 atatcgactg caagagtggc aaagcccaca tcgccctgtt ccatcattcc tgccactgcg
    12661 ggcagcaaca ccagcgtcag aaccattacc aggtcttcca caatcaacca accgatggcg
    12721 atttgcccac gctgactgtc aattaattgc cgttcttcaa gtgcgcgcag taacaccacg
    12781 gtactggcgg tggaaagaca taaaccgaac acgataccgg tcattaacga ccagcccagc
    12841 acggcggaga gcgccatacc cagcagcgtc gccacggcta tctgggcgat cgcgccggga
    12901 atggcgatgg actttaccgc catcaaatcc ttgagcgaaa aatgcaaacc gacgccgaac
    12961 atcagcaaaa taacgcccag ttcagccagt tccggcgcaa gcttggtatc ggcaacaaag
    13021 cccggagtga atggtcctgc cagcacaccc gctaacagat atcccaccag aggagaaata
    13081 cgtagtttat tggccagcat gccgaggata aaggcgagca caaggccgcc aacaatggtg
    13141 gtgataagcg gggtggcgtg atgcattccg tctccttttc ctggtggtta ttgtccattt
    13201 ttggccggga aaaccaaaat tacaggtaat agtttatgac aatttcattg atgatgttca
    13261 tgaataatta ttgaattttg cagaaaaatg gaattagctg caaaaaaagc acggatcgga
    13321 aaacagaagg cggtttaaca gaggaaaggc ttatggcgac tgtaaaacga tgcagccaaa
    13381 gtttgccttt ggctgcaatg aaatcagtct ttatgccggt tatcaggcag gaatatggtc
    13441 aacatcccca atagtggcag gaaagcacag attttataga ctaactcgat actggtgtga
    13501 tcggcgataa gccccagaac tgccgctccc agacctccca taccaaaagc aaaaccgaaa
    13561 aagagtccag aaaccatacc gatacgtccc ggaagcagct cctgagcgta aaccagaatg
    13621 gcagagaatg ccgaagcgag gataaatcca ataatcaccg ttaaaacccc cgtccagtgc
    13681 aggctggcgt agggtaaaat cagcgtaaac ggcgcaacgc cgaggataga gccccaaatc
    13741 acatatttcc gcccaatttt atcccctaca ggcccgccga tcaccgtacc tgccgcaacg
    13801 gcaaacagga aggcaaacag atgaagctga gcattctgga tagataatcc gaatttttgc
    13861 atcagataaa aggtgtaata gctgctgatg ctcgccatat agaaatattt cgagaaaatg
    13921 aggattaaca gaatgctgac cgccagtacc actttgttgc gcggcagtgg attgataatc
    13981 gtcgctttgg gttttccttt attcattcgg tgctgtgccg agtaccaacg gctgatttgc
    14041 gccaacacca cgatcgccag cagtgccgca agcacaaacc aggcaacgtt gcctttgcca
    14101 taaggcgcga taatcaccgc cgccagcaag ggtcccaggg aactgccaaa gttgccgccg
    14161 acctgaaaga tagattgcgc caggccatgc cgcccgccgg aagccatacg ggctacgcga
    14221 gaagattccg gatgaaagac cgatgaaccg gtaccgacca gcgccgccgc cagcagaact
    14281 gcgccaaaac tgcccgccag cgcaagcagc accagaccac ttaaggtaaa gcacatgcca
    14341 attggcaacg accacggcat cggatattta tcggtccagt agccgaccac tggttgcagt
    14401 agcgaagagg cgagctggaa ggtgagggtt atcatgccaa tctgcataaa tgtcagagaa
    14461 aattctgact gaagcagcgg ataaatcgcc agaatcagcg attggatcat gtcgttcagc
    14521 agatgtgaga ggctgatagc acctaaaata ccaaacgatg ttcgggcctt ggtcgttgac
    14581 gcagccgcgc ccgccacagg ctggggttgt tcactcattg ccataggaaa gtcacttttt
    14641 cagggttgcg atgtaaagaa tgatcttatt tgtgattatt acccgactaa catacctgta
    14701 tgcgtcgcct gaaggaagtc tcaacgccga atacagaatt tctaatctgg atgcagattt
    14761 atcttcaccg gacgcagact tgtctatgat gtcgcgtcat actatttttc aacacgttga
    14821 aatcaggtca gggagagaag tatgaaatta ttgcagcggg gcgtggcgtt agcgctgtta
    14881 accacattta cactggcgag tgaaactgct ctggcgtatg agcaggataa aacctacaaa
    14941 attacagttc tgcataccaa tgatcatcat gggcattttt ggcgcaatga atatggcgaa
    15001 tatggtctgg cggcgcaaaa aacgctggtg gatggtatcc gcaaagaggt tgcggctgaa
    15061 ggcggtagcg tgctgctact ttccggtggc gacattaaca ctggcgtgcc cgagtctgac
    15121 ttacaggatg ccgaacctga ttttcgcggt atgaatctgg tgggctatga cgcgatggcg
    15181 atcggtaatc atgaatttga taatccgctc accgtattac gccagcagga aaagtgggcc
    15241 aagttcccgt tgctttccgc gaatatctac cagaaaagta ctggcgagcg cctgtttaaa
    15301 ccgtgggcgc tgtttaagcg tcaggatctg aaaattgccg tcattgggct gacaaccgat
    15361 gacacagcaa aaattggtaa cccggaatac ttcactgatg tcgaatttcg taagcccgcc
    15421 gatgaagcga agctggtgat tcaggagctg caacagacag aaaagccaga cattattatc
    15481 gcggcgaccc atatggggca ttacgataat ggtgagcacg gctctaacgc accgggcgat
    15541 gtggagatgg cacgcgcgct gcctgccgga tcgctggcga tgatcgtcgg tggtcactcg
    15601 caagatccgg tctgcatggc ggcagaaaac aaaaaacagg tcgattacgt gccgggtacg
    15661 ccatgcaagc cagatcaaca aaacggcatc tggattgtgc aggcgcatga gtggggcaaa
    15721 tacgtgggac gggctgattt tgagtttcgt aatggcaaaa tgaaaatggt taactaccag
    15781 ctgattccgg tgaacctgaa gaagaaagtg acctgggaag acgggaaaag cgagcgcgtg
    15841 ctttacactc ctgaaatcgc tgaaaaccag caaatgatct cgctgttatc accgttccag
    15901 aacaaaggca aagcgcagct ggaagtgaaa ataggcgaaa ccaatggtcg tctggaaggc
    15961 gatcgtgaca aagtgcgttt tgtacagacc aatatggggc ggttgattct ggcagcccaa
    16021 atggatcgca ctggtgccga ctttgcggtg atgagcggag gcggaattcg tgattctatc
    16081 gaagcaggcg atatcagcta taaaaacgtg ctgaaagtgc agccattcgg caatgtggtg
    16141 gtgtatgccg acatgaccgg taaagaggtg attgattacc tgaccgccgt cgcgcagatg
    16201 aagccagatt caggtgccta cccgcaattt gccaacgtta gctttgtggc gaaagacggc
    16261 agactgaacg accttaaaat caaaggcgaa ccggtcgatc cggcgaaaac ttaccgtatg
    16321 gcgacattaa acttcaatgc caccggcggt gatggatatc cgcgccttga taacaaaccg
    16381 ggctatgtga ataccggctt tattgatgcc gaagtgctga aagcgtatat ccagaaaagc
    16441 tcgccgctgg atgtgagtgt ttatgaaccg aaaggtgagg tgagctggca gtaatccgaa
    16501 agtgccggat gtttgcatcc ggcacaatgc ttaatcgcgg cgggcgatat cagcaaattt
    16561 ggcatcgagg atctttgcca gatcgcctgc cgccagttcg atatccagtc cgcgcttgcc
    16621 gccggaaaca taaatagtgg caaattcttg tgcgggggcg tcgataatcg ttggcagacg
    16681 ttttttctgc cccagtgggc taatcccccc aaccaggtat cccgtcgaac gctgcgcgac
    16741 catcggatcg gccatctcaa ctttcttggc acccagcgct tttgctactt ttttaagatc
    16801 cagttgaccg gcgaccggcg taacggccac ggcaaggtgt ttcatatcac cgttcactgc
    16861 caccagcagc gttttgtaga cctgatccgg attcaaacct aattttttga cgacttcatc
    16921 gccaaaattg gtttcagccg gatcgtgctc gtaggtatgg atttgaaacg aaatcttgtt
    16981 tttttcgagt aatttaactg cgggtgtcat ggtaattttc ctgccttaaa caaaaaaacg
    17041 aagcaagcat acgcctgatg gcggtccaaa aaaaagagtc atcttgccta agagtattgg
    17101 caggatggtg agattgagcg acaatcgagt tacaccgtcg cttaaagtga cggcataata
    17161 ataaaaaaat gaaattcctc tttgacgggc caatagcgat attggccatt tttttagcgc
    17221 aacatttgcg gtaaattccc ttctccatac aggtgtagtg caccgaccgc gaccacatat
    17281 cgccccggcg gcatggcgcg taatttatcc cgccaggcga gatttcgctg atgcatcagc
    17341 acatcgtaca gcgactgact gaacgtattg ggcagcgtta tttcattatt ttgcggcggt
    17401 gcattcagcc accagctcat catttgttgc agcaaccgtg cattggtgtg ccagtgggtc
    17461 agtgtatcgt ccagcagcgc cagtcctttg tcagggagct ggagcaacat ggcaatctgg
    17521 ttttcagccc cttccagttc aatcacgggt ttatgttgtt gcttcgccgc ctgcaatagc
    17581 tggtaatcga taccgtattc tgcccgcagc cccagttttt gtgcctgcgt cgcctgaaga
    17641 accatcgcga tttgccacag cggttgggta gaaaagagtg agggagaaat gcccatctcc
    17701 tggctaatgt gctgcagatt ttggagttgc tcctcgctaa tgcgctcttc cagcgcctcg
    17761 caggcaggca aattggcaaa aggcgtatcg ctggtggaaa catccgcctc gacgatcagc
    17821 gcatcggcgt ttttgagctt tttgagcaaa cgggtgggca ggggagccat atcgtggcta
    17881 cccatatgaa tactgccaat cagatgaaaa tggcgattgc cgggaagggt gatatcgatg
    17941 gcaggccagg tgtaatgatt accgcgcagc gcggcccaaa gcgtttttac ccggtacaac
    18001 agatccatac gacctccctt tgtgaaatat catgctagcg cgcggggagg gatggtgcaa
    18061 atggagttgg ggacttgttt aatgtgtttg tatgattcag tatattcttg tatcgctatt
    18121 cacagggaag caacagttaa aaactatgaa acaggcaaca agaaaaccga cgacacccgg
    18181 agatattctt ctctatgaat atctggtacc gctcgatttg aaaatcaatg agttagcaga
    18241 gttgctgcat gttcatcgta atagcgtcag tgcactgatc aataacaatc gtaaactcac
    18301 cactgagatg gcattccgtc tggcgaaagt ttttgatacc acagtcgatt tttggctaaa
    18361 cctccaggcg gcagttgatc tttgggaagt tgaaaacaac atgcgcaccc aggaagaatt
    18421 gggacggatt gaaacagtgg ctgaatactt ggcacgccgt gaagagcgtg caaaaaaggt
    18481 cgcgtaaggc caaaacatcg cttcatctcg taggccggat aaggcgttta cgccgcatcc
    18541 ggcagtcatg cgtcgatgcc aaatgtgcca ccctaaagca gcgcatccgc aatgacatac
    18601 ttattccttc ggtttaaacc gcagcaaccg gttggcgtta ctcactacgg taatcgacga
    18661 gagcgccatt gccgctccgg caactaccgg gttaagcagt gttccagtaa acggccacaa
    18721 aataccggcg gcgaccggaa taccgatact gttgtagata aaggcaccga gcaggttctg
    18781 cttcatgttg cgcagcgttg cgcgggaaat cgcgagcgca tccgcaacgc ccatcaggct
    18841 atggcgcatc agggtaatcg ccgcggtttc aatggcaaca tcactgccgc cccccatcgc
    18901 aataccgaca tccgcctggg ccagcgctgg cgcatcgtta atgccgtcgc ccaccattgc
    18961 cacctgacgt ccttcacttt gcaggcgttt aatcgcttcg gctttaccgt ccggcagcac
    19021 cccggcgatc acttcatcaa tcccggcttc tttggcgatc gcattggcgg tggttgggtt
    19081 atccccggtc aacatcacca gacgatatcc cgctttatgc aggcgttgca gcgccgccac
    19141 gctatcacta cgcaatggat cgcgtactgc cagcagggct accgctttcc cgtcaaccgc
    19201 cagcagcaca ggcgttgccc cttgcgatgc ctgagcagta atctccgctt cgatagcttt
    19261 ggtaccaacc tgttgctcat ttaacagcgc ctgattgccc agcaataacg catgaccttc
    19321 agcttcaccg ctcacgccca gcccgcgcaa tgtgcggaaa ccgttgacct gcggcagctg
    19381 catatcacct gctttatcga ggatcgcccg tgccagcgga tggctggaac cttgctccag
    19441 tgccgccgcc agacgcaatg cctgcgcttc atcaacatca gcaaacgttt tcactgcgac
    19501 aacctgcggc ttcccttcgg tcagcgtccc ggttttatcg aacacgacgg tatcgagtgt
    19561 actggcgcgt tgcagcgcgt cggcgtcccg caccagcacg ccaaactcag ccgcccgccc
    19621 gacgccggaa ataatcgaca tcggcgtcgc cagccccagc gcacacggac aggcgataat
    19681 cagtaccgtg gtggcaatca ccagggtata aacaatttgc ggtgccggac caaagaagta
    19741 ccagattgcc gcactgacca gcgcaatcac caccaccaca ggtacaaata cggctgagat
    19801 tttatccgcc agctgaccga tttctggctt gctgctctgg gcctggcgca ccatgcgaat
    19861 gatccgtgac agcgtggtat ggctgccaac cgcactggca cgaaacagca cgctaccgtc
    19921 ctgtaccact gtcccggcat ggacgctttc gccttcgcct ttttgctgcg ggattggttc
    19981 gcccgtcagc atcgcttcat ccagccatgc ttcgccctgg gtaatctcgc catctaccgg
    20041 cacgcgatcg ccggtcgtca ggcgcagcaa catacctggc tgcacttccg ccagaggcac
    20101 gcttttttca ccttcgtcag taaccaggcg cgccgtcggt ggggttaaat cgagtaattt
    20161 ctccagcgcc ttagaagaac gctgacgtgc gcgtgcttcc agcatatggc cgagattgat
    20221 cagaccgata atcatcgcgc tggcttcgta ataaagatgt cgcgcttcca tcgggaacca
    20281 ctgcggccac aggttgacgc tcatcgaata gagccacgcc acgccagtac ccagcgccac
    20341 cagcgtatcc atcgtcgctg caccgttcag caggcttttc catgcactgc ggtaaaaatg
    20401 gccgccggcg aaaaccatca ccgccagggt tatcaggccg ataaccaacc acaggctgcg
    20461 gttgtcagcg gtgaccatca tgttatcgcc aatcatcccc cagaccatca ccgggatacc
    20521 caccgccagt gcgacaattg cctgccagcg gaagcgcttc atcgtagcaa cggcggtttc
    20581 ctgctggcgc tcacggcgtt tcgcgtcatc ttcaatcgct tccgcgccgt agcccgcttt
    20641 ttccaccgcc tgcactaaat cttgtgggga ggcactgccc atcaccagcg cagtacgctc
    20701 cgccaggttt acccgtgcct gagtgacgcc cggtacgctt tgcagcgcat tttgtacgcg
    20761 ggtgacacag ctggcgcagc tcatgccgct cagcagcaac tgctggctgt catcgtcatc
    20821 ggcggtcgct gccggaagcg cctcagaaac cgctgtcagt gcttccgacg ggattgatga
    20881 ctccgccagc ggtttagcct ttgggtggct tacagatgcg tcataacccg cttgtttgat
    20941 ggtttcgatc agctgttctg cactggcagt cccggtaacg tgcgcttcag tgatagacac
    21001 atccgcttgt tccacatccg ggcgctgttc gagactttct ttcacgcgtt taacgcagtg
    21061 accgcaggac aggccgtcca gggtcaggtc gataatttgt gacataaaac actcctttaa
    21121 gacagttttg actggctgtg ataaaggtta aaccttccag caaggggaag gtcaagaaat
    21181 taataaacca ggcgagtaaa agtccgtaaa gattaaaaaa ttggctcaat ttgcatcagg
    21241 attagacatt tatctctttg ttttcctgta gttaagttgc gggtgctaag ttaaatcaag
    21301 atgcctgaaa atcggcaccg gggtgaggaa ttacctcccg catctataaa aaggagttaa
    21361 caaaagatgt tagatgcaaa caaattacag caggcagtgg atcaggctta cacccaattt
    21421 cactcactta acggcggaca aaatgccgac tacattccct ttctggcgaa tgtacctagt
    21481 caactggcag cagtggctat tgtgacctgc gatggcaaca tctatcgcgc gggtgatagt
    21541 gattaccgct ttgcgctgga atccatctcg aaagtctgta cgttagccct tgcgttagaa
    21601 gatgtcggcc cgcaggcggt acaggacaaa attggcgctg acccgaccgg attgcccttt
    21661 aactcagtta tcgccttaga gttgcatggc ggcaaaccac tgtcgccact ggtaaatgct
    21721 ggcgctattg ccaccaccag cctgattaac gctgaaaata ttgaacaacg ctggcagcga
    21781 attttacata tccaacagca actggctggt gagcaggtag cgctctctga tgaagtcaac
    21841 cagtcggaac aaacaaccaa cttccataac cgggccattg cctggctgct gtactccgcc
    21901 ggatatctct attgtgatgc aatggaagcc tgtgacgtgt acacccgtca gtgctccacg
    21961 ctcatcaata ctgttgaact ggcaacgctt ggcgcgacgc tggcggcggg tggtgtgaat
    22021 ccgctgacgc ataaacgcgt tcttcaggcc gacaacgtgc cgtacattct ggccgaaatg
    22081 atgatggaag ggctgtatgg tcgctccggt gactgggcgt atcgcgttgg tttaccgggc
    22141 aaaagcggtg taggtggcgg tattctggcg gtcgtccctg gcgtgatggg aattgccgcg
    22201 ttctcaccac cgctggacga agaaggcaac agtgttcgcg gtcaaaaaat ggtggcatcg
    22261 gtcgctaagc aactcggcta taacgtgttt aagggctgat catgatgaac acggaaggta
    22321 ataacggtaa caaacctctc ggtctatgga a
//
LOCUS       DAZGJW010000071        22145 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZGJW010000071 DAZGJW010000000
VERSION     DAZGJW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.071"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..792
                     /locus_tag="ACHKIK_002770"
                     /pseudo
     CDS             1..792
                     /locus_tag="ACHKIK_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1065..1649)
                     /locus_tag="ACHKIK_002771"
     CDS             complement(1065..1649)
                     /locus_tag="ACHKIK_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083914.1"
                     /translation="MNMKNSSFLDEGKEPVIERIFRLAERYKSRSEAARAWGINVQTL
                     QNYYKRRNNQPPPQPRKHHLQNIAEREHVSMEWLVNGYGDEPSMKKEPIEQTKRTKKQ
                     RIDDSDSIIMRAWDSLTSSEQEILSNLLIRKGAELLTVLLDTDIQKLHSLSGVKRALA
                     LSLQELPDDTVREIYEECEAKVNHLNVTQKKAGA"
     gene            1817..2065
                     /locus_tag="ACHKIK_002772"
     CDS             1817..2065
                     /locus_tag="ACHKIK_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9083915.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            2067..4157
                     /locus_tag="ACHKIK_002773"
     CDS             2067..4157
                     /locus_tag="ACHKIK_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO9083916.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKVDESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVLKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFTALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPVKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVTVQQPVADDEPDEEYERNFQRGL
                     QLLEAKLKENDPLA"
     gene            4229..5161
                     /locus_tag="ACHKIK_002774"
     CDS             4229..5161
                     /locus_tag="ACHKIK_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9083917.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            5164..5385
                     /locus_tag="ACHKIK_002775"
     CDS             5164..5385
                     /locus_tag="ACHKIK_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083918.1"
                     /translation="MAVFNIPDIYGRFYLVNFDSVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATDVYSGICRSVGAKQVS"
     gene            5398..5652
                     /locus_tag="ACHKIK_002776"
     CDS             5398..5652
                     /locus_tag="ACHKIK_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083919.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            5654..5935
                     /locus_tag="ACHKIK_002777"
     CDS             5654..5935
                     /locus_tag="ACHKIK_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFO9083920.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            5932..6204
                     /locus_tag="ACHKIK_002778"
     CDS             5932..6204
                     /locus_tag="ACHKIK_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083921.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFAELVAMKASALMKDMSMRSGVIKGNEFNQQG"
     gene            6209..6502
                     /locus_tag="ACHKIK_002779"
     CDS             6209..6502
                     /locus_tag="ACHKIK_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083922.1"
                     /translation="MSKVRVIFEFKHVSHDEKPAGNDCVEVHEKIGVDVKTERDTNNR
                     PTSLCGVYASILQYHSPEIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ"
     gene            6514..7044
                     /locus_tag="ACHKIK_002780"
     CDS             6514..7044
                     /locus_tag="ACHKIK_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO9083923.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGNPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            7142..7684
                     /locus_tag="ACHKIK_002781"
     CDS             7142..7684
                     /locus_tag="ACHKIK_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083924.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGALPAGWRKRDDVAFIGDGPCFIARPDESCPEGPAIAAMIEA
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNMDSWWTPSLSRDALCVVFKVGAYGREIKG
                     GIPEECQEIKHSEYVALTEE"
     gene            7688..8221
                     /locus_tag="ACHKIK_002782"
     CDS             7688..8221
                     /locus_tag="ACHKIK_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083925.1"
                     /translation="MIGAKVLEGVKNWLSIYGRLTCGILAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQEGAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITSRVLRY
                     LIVDRTAAPAGISAEVA"
     gene            8221..8736
                     /locus_tag="ACHKIK_002783"
     CDS             8221..8736
                     /locus_tag="ACHKIK_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083926.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFCFKGLRFMDDKYPFHRDLWRKPTASNGWSCTPRTSRIPKALRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAIMFGPVKWFCVDDVIYLECGVKPANQKMTEILSD
                     EFYAAEKRVRG"
     gene            8740..9291
                     /locus_tag="ACHKIK_002784"
     CDS             8740..9291
                     /locus_tag="ACHKIK_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFO9083927.1"
                     /translation="MMILQPMGRKGRAPAHVRAWTPEEDALLIALYPSTPVKDIAVRI
                     KRSFWGVHNRIVLLRGTYPELLKCKRLRFKPDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVRLIRELRDAESPRRLTFREIGEKFELSE
                     GTVSFIYHRRRTAEDVVLRELMP"
     gene            9288..9599
                     /locus_tag="ACHKIK_002785"
     CDS             9288..9599
                     /locus_tag="ACHKIK_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083928.1"
                     /translation="MITTLFVESDEPLVCAVGMPVCGGTLTGVYFGDLRGYPWHSLND
                     AFPPDMEAVVLIVQYGDQQELRIGHMGYEGFFIDEETGACLEDEDGQVTHWCHISALP
                     E"
     gene            9614..9964
                     /locus_tag="ACHKIK_002786"
     CDS             9614..9964
                     /locus_tag="ACHKIK_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083929.1"
                     /translation="MRSYIDNEKLKTISDCLSLLAKIKETIEEIKFQLEYEPCGDDTL
                     RNSARKALAVFQKQRRTVEYRLAVLRQEEKERNIRRHELVNDFLVRELKERVPESVFF
                     ECEAVARSKALETD"
     gene            9980..10312
                     /locus_tag="ACHKIK_002787"
     CDS             9980..10312
                     /locus_tag="ACHKIK_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083930.1"
                     /translation="MSIELRSSYEYRKILIAGGMKPEDAEKIVSFMDKECDKRDMPEI
                     IMDDMILHSAVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIHMILS
                     SMEDKANE"
     gene            10305..10502
                     /locus_tag="ACHKIK_002788"
     CDS             10305..10502
                     /locus_tag="ACHKIK_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083931.1"
                     /translation="MNSLPAGWARPLMARKHHFFKTGENISICGRWLYLAHNREPDTF
                     ESPDDCAECRRRVNKEKDNGQ"
     gene            10492..10788
                     /locus_tag="ACHKIK_002789"
     CDS             10492..10788
                     /locus_tag="ACHKIK_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083932.1"
                     /translation="MDNSSLLFWCLYITSFFGAFVITRWLCRKIICFFDKRHPVERAA
                     DALIQQAIVLYNGEFFCRITSRDGWHIMIIPPTHHARWDEAEKAFHVRKKVNTV"
     gene            10785..11294
                     /locus_tag="ACHKIK_002790"
     CDS             10785..11294
                     /locus_tag="ACHKIK_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO9083933.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTSALLAATGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQNPVNRALKPGTVTAKIRAIWKVMHRQGFITDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMGMPERTLMGYDAVC
                     RQYEKSLPR"
     gene            11364..11789
                     /locus_tag="ACHKIK_002791"
     CDS             11364..11789
                     /locus_tag="ACHKIK_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO9083934.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDVLKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLVRTQPPLF"
     gene            11861..12361
                     /locus_tag="ACHKIK_002792"
     CDS             11861..12361
                     /locus_tag="ACHKIK_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9083935.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            12396..12824
                     /locus_tag="ACHKIK_002793"
     CDS             12396..12824
                     /locus_tag="ACHKIK_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083936.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEGQQSV
                     VRRSLEAVPAASAPVPDDVADRVRRAVCEIRACEAGADPR"
     gene            12808..13026
                     /locus_tag="ACHKIK_002794"
     CDS             12808..13026
                     /locus_tag="ACHKIK_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083937.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            13037..13264
                     /locus_tag="ACHKIK_002795"
     CDS             13037..13264
                     /locus_tag="ACHKIK_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO9083938.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRRAMDAPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            13245..13553
                     /locus_tag="ACHKIK_002796"
     CDS             13245..13553
                     /locus_tag="ACHKIK_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFO9083939.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTQ
                     "
     gene            13550..13840
                     /locus_tag="ACHKIK_002797"
     CDS             13550..13840
                     /locus_tag="ACHKIK_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFO9083940.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRATVPGVKKPRARG"
     gene            13843..14424
                     /locus_tag="ACHKIK_002798"
     CDS             13843..14424
                     /locus_tag="ACHKIK_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFO9083941.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKA"
     gene            14424..16088
                     /locus_tag="ACHKIK_002799"
     CDS             14424..16088
                     /locus_tag="ACHKIK_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9083942.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            16088..17677
                     /locus_tag="ACHKIK_002800"
     CDS             16088..17677
                     /locus_tag="ACHKIK_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFO9083943.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAVLDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIP
                     DPWVREQTGIPQPAPGEAIFRVRQSGNEPAQTDKEMPPEKQEKTEQTALAARLPEAKS
                     SPRDELDDMGDAVPARRLQEAIDPVLEPVIEAIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGTGD"
     gene            17661..18992
                     /locus_tag="ACHKIK_002801"
     CDS             17661..18992
                     /locus_tag="ACHKIK_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFO9083944.1"
                     /translation="MAQGIDLGYAATLPSKEAVAYFRAKGAHISWNWFETDADVHARS
                     FTAAKVARLDVLTTLQAEVQRAVDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGQVFRYDDPFWNTHYPPNGWNCRCRVRPLSQTRLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSMKHQNDGIALTPEDFGRLPAMLAKPKAVLWDKRHNNLMYIVESKDS
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGDID"
     gene            19114..19587
                     /locus_tag="ACHKIK_002802"
     CDS             19114..19587
                     /locus_tag="ACHKIK_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO9083945.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAASLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            19764..20888
                     /locus_tag="ACHKIK_002803"
     CDS             19764..20888
                     /locus_tag="ACHKIK_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO9083946.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINRATVERMVSRVVALNQPVKIDYNHQTLIKGHQAPAAGFVMASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDENTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPAGGIIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQVLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTSLQTD
                     TVTVPDKKTATAALSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            20888..21835
                     /locus_tag="ACHKIK_002804"
     CDS             20888..21835
                     /locus_tag="ACHKIK_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO9083947.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAVQPDELVFAALRDGISAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            21879..>22145
                     /locus_tag="ACHKIK_002805"
     CDS             21879..>22145
                     /locus_tag="ACHKIK_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFO9083948.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVTPEQLARLEEEPCLTVRILQTSADDAGSVAGVVHAVA"
BASE COUNT         5377 a         5448 c         6332 g         4988 t
ORIGIN      
        1 tttaactgtt tttttcatcc gcttgtgggg ctgccgccgg tgtatgccgc aggactggcc
       61 gccatgcagg ggcatcatat tcaggcaaat tcgacgtatt ttttcagaaa tggcggcagg
      121 ccgtccgggg tgattgagat ccccggcagt ataacggaag aaaatgcgaa aaaactgaag
      181 agcaactggg acagcgggta taccggcgaa aatgcgggga aaacggccat tttgagcaac
      241 ggggcaaaat acaaccccac gacgttttca ccggtggatg cgcagacggt ggaacaactg
      301 aagatgaccg ctgaaattgt ctgttcggtg ttccgtgtcc cggcctacaa gattggcgtt
      361 ggccacccgc cttccagtga caacgtggag gcgctggagc agcagtatta ttcccagtgt
      421 ctgcagacgc tgattgagtc cattgagctg ttactggatg aggcgctgga aacgggggaa
      481 aacgagagca cggagtttga cgtcaccacg ctgctgagaa tggacagcga acggcgcatg
      541 aaaacactgg gtgaatcggt gaaaaatacg cttctcacgc ccaatgaggc ccgtaaacgt
      601 gagaacctgc cgccccttgc cggcggtgat gcactgtatc ttcagcagca gaactacagt
      661 ctggaggcgc tgtcccgtcg tgatgcccgt gaggacccgt tcgcgtcggc cgggaaaaca
      721 gtttcagcac aactgcctga cggcgcatct gacggtaata aggcaatcag tgaaacagag
      781 catgatgcag tgtgtaacca ggtgaatcat ttgataccaa cgagggtttt agtgaaagga
      841 ggtgaaatca cctgtaagat actgaaataa aacaggtgat gtctggatga gcgcttaaag
      901 tctatttgat accgattgtg caataattaa tcgtttattc taaaatcggc aaaacttttg
      961 atactacgga ttgacgctta aaatggcttt aaaaaggctt aaaacgtttt tctccagatt
     1021 gtctttaaag gagtgtacta cagcacaaaa attgcaccac tcccttacgc accagccttt
     1081 ttctgggtta catttaaatg attaacctta gcttcacatt cttcataaat ctctcttaca
     1141 gtatcatcgg gtaactcttg tagactcaat gctagtgctc ttttgactcc gctcaatgag
     1201 tgcagttttt gaatatctgt atcaagcaaa acggttagta gttcggcccc ttttctgatc
     1261 aataagttgg acaaaatctc ttgttcagat gaagttagcg agtcccacgc cctcatgatg
     1321 atggaatcac tatcatcaat tctttgtttt tttgttcttt ttgtttgttc aattggttct
     1381 tttttcatgc ttggctcatc accataacca ttgactagcc actccataga tacatgttct
     1441 ctttctgcaa tgttctggag atggtgcttt cttggttgtg gtggcggctg attgtttctc
     1501 cttttgtagt agttctgcaa tgtctgaacg tttattcccc atgctctagc cgcctcactt
     1561 ctcgatttgt aacgctctgc aagacgaaaa atccgttcga tcactggttc ttttccttcg
     1621 tcgagaaaag aactgttttt catgttcatg ctctcaggtt cctttgtatt tatcttattg
     1681 atattaataa caaaaacacc gcaaagaata aaaagaacaa aataccttga tttggttctt
     1741 ttgtttgttc tattatttac ccgtacagat atccgcacga atatccgcgc gggtaacttt
     1801 ttagaataga ggaagtatga gtagaagaga agtttcagat cacgattggc cgcctgaaat
     1861 catcaaggcg cgcttacaca tggctggctt atcactgcgc tctttatctt taaaggctgg
     1921 ttacagcagg gattcgctga aaagcgtatt acgtactcct tgccgaccgt atcagcaaat
     1981 aattgctgat gctcttgggg tatcgcctga agaaatctgg cccagtcgat accaggttaa
     2041 gagctatatg agaaaggcgt catgatatgt atgtcattgc aaaagaactg attggcgcgc
     2101 ccggaatgcc tgctacaaca aaaggtattc gccaggcatt acaacgttac gtacaaggga
     2161 aaagctgttg ttcccgtcgt cgctcaggct ctaaagcaac tgaatacagc atcgactgtt
     2221 tacctgaagt gacgcagcag gcattacgtg aacgctacgc cctgcaactg atgactcaaa
     2281 aagtcgatga atcaccggct ccagtggtga caaaggccag acgctcgcct gccgtggttg
     2341 atgcggtgga ggcatatcgc ggatcaccac aactgatggt cgaacgcctt aatgccctga
     2401 ctgaaaacca gcgccaggtg gctgatgcac gaatcgcgat cgtcagcgaa gttctgaaag
     2461 tcgcgcaaca acctggtttc agctgtgcga aggctatccg gtttatcgtt gacaacctgg
     2521 cacgttcgca gctggacgag cgcattgtgg caatggttga gacggcgaac gccaaaaagg
     2581 gaaacagccg cgcgttaagt gaaatcacgc tgaaacgctg gattgcggcc tttaacaagg
     2641 cgcagaacgc cgctgaacgc ctgcttttac tggcaccggg taagcgccag gaaataaaag
     2701 ccgaagatat taactggctg cccgaatttc tggcgcagta tcgccagtca aacggccgac
     2761 caatgaccga ggcttacgag gattttgtcg ctgaatggca gcaccggcac gctgatgagc
     2821 cttatatgct cgatatcatg ccgtcttatg acaccattcg ccgcgcaatg aagaaactgc
     2881 cggaagtggt gaaacaaaaa ggccgggtga ccggcagtga ataccgccag cttgagggat
     2941 tcacgcgccg cgactggtcc agaatgccgg tgaattatgt ctggattggt gacggtcacg
     3001 gcatgaagct gaaatgcgca cacccggttc acggtcggcc atttgcaccg gaagtgacct
     3061 ttgttatcga cggtggcacg cgctttgtgg tgggctggag ccttgacctg gctgaaaatg
     3121 ttttcgccgt agccggtgcc atacagcacg gcattcgcca tcacggcaaa ccgtttctgt
     3181 attactcgga taacggctcc ggggaaaccg ccgacatcct ggataaggag gttgtgggga
     3241 tactgccgcg actggggatt aatcacccga ccgggattgc cggtaatccg cagggacgag
     3301 gcattatcga acggcttaac cgcacattac cgatgcgcat agcccgtaaa taccgcacct
     3361 atttcgggaa aggtgcagat cgcgagacgt tacgcaaaac caaccgcgat ttacgctcgg
     3421 catttactgc cctgcaacag ggcaaacggc tgaacgcccg gcagcagtca gcgatgcgtg
     3481 atttaccgtc ctggtctgaa ctgattgatg ccattcgtga cggtgttgag tggtacaaca
     3541 accggccgca cgatgaatta ccggtgaagc cgaacggcaa gcattacagc ccggcggagt
     3601 tcagaaaaaa acgcctggcg gaagaggaca cggaaattga atggctgtcc gatgttgagc
     3661 tgcgggatat gttccggccg atggtggaac gccctgtaag acgctgtgaa atacgctggc
     3721 tgaataatat ttactacgcg cccgagctgc gtgacgagca tggccgtaag gtgcttatca
     3781 gctatgacat tcatgatgcc gaacgaatta ccgtacgtcg cctggatggc agcgtgattt
     3841 gcgaggcggt atgggacggc aataaacgcg aagccttccc ggtcagcgcg gaatactaca
     3901 aacagcagca acgccttaaa ggcatgcgta aacgcgcaga ggaaaaaatc cgtgatgccg
     3961 aggatgaggt tgtcaacgtg ctggagcaca agccgcagga gccctggctg gaaaacatat
     4021 accgccctgt gggtaatacg gtgaccgttc agcaaccggt cgctgacgat gaacctgatg
     4081 aagaatacga gcgtaatttc cagcggggat tgcagttgct ggaagcgaaa ttaaaagaaa
     4141 atgacccgct ggcctgaaat aaaaaaataa cccgagcggc gactcaggtt atttgattaa
     4201 acaaggtatc aaatgagagg ttaataatat gactgatatt aacgatgtaa tcaagaccat
     4261 tgatgaactt attgatggcg gcgtactgac gcagtatgcc atcgccagag aggcgggaat
     4321 ttccgacggc acattatcgg ctttccgcaa ggggaaatat aaaggcgata acgccgctgt
     4381 ggctgcttcc ctgcgttcct ggtatgaaaa ctggaataaa caaagcgcac tgccggaacc
     4441 gccgcagttt gtggaaacgc agacagtcca ggagctgcgc gcactgtttc aggcggttcg
     4501 tctgatgggc tgtattaacg ttattgtggg cgtaccgggt gtgggtaaaa cggccactgc
     4561 ccgtaattac tgccaggagc aaccaaacac ctggatgatc accctgtcac ccgcgcactc
     4621 cagcgtcacg gagtgtctgc tggagctggc cgatgcgctg gggattgatt acacccgcgc
     4681 gaacaaaggg gcattatccc gcgccatccg ccgtcgcctg atgggaacgc gtggactggt
     4741 gattgtggat gaggcggatc atctgggtat tgacggtctg gagcaactcc gggcaattca
     4801 ggacgccacg gggatcggga tggtgcttat tggtaacccg cgcggattgt ttaaaggtgg
     4861 acgccgcgcc tttgatgatt tatcgcgcct gttcagccgt cttgcccgta caaaacaact
     4921 tcgcaaggcc aaaaaggcgg atgtgctggc cattgccagg gcgtggggga tcagtggtga
     4981 ggccgagctg gccgtcatgc aggctatcgc tgaaaagccg ggagcgttac gcgttctgac
     5041 acatacgctt aaccaggcgt ggctcaccgc cagcggtgaa ggcgcggcgc tgacagaaaa
     5101 acatattaat gcggccttta aagaggttta taccaaccct gaattactct cacaggtgtg
     5161 attatggcgg tatttaatat tcctgatatt tacggacgct tttacctggt taatttcgat
     5221 agcgtgaagg tgatttcact ggccgaaaat aaagaatgtg gcgatttact ttttgaattt
     5281 aatgaccgca cacgaatggt gatatctgcc ggacttgatc gcgaaggtgc gacagacgtt
     5341 tacagcggaa tatgccgttc tgttggtgcg aaacaagtca gctaaatgag gtgttatatg
     5401 aatatgcaat cctgcggtaa caaaatgaat ttattcgact ccctgaacag cgcgcgccgt
     5461 ctgaccgaac ttgccggtgc ggtactggaa cgcagtaagc gctatccgca acgttttgca
     5521 ctgaaaacca cgccgccggt aggcaacgtg cagggaaccg gtgaaattga aatcaccata
     5581 cagaccaacg gcctgcgccg ccgtgtgaag gccacccgca tcagcggctg cacggtttac
     5641 tgggaggtgt gaggtgaaaa aaaatctcat tgcatgggcg tggtcgagtg gtcttattga
     5701 gtttggttac gtcctgccgg aaggtgcatt gccgatagtt gccggaaagc ctgccacggt
     5761 acggcatgtg attgaggtta tggcgcgtca tggacgtgat gaacaggagc agttactggt
     5821 tccggggata ccggaagcgg tgacggagga agaagccttt aatgccatga ttcggttctg
     5881 ccgtgaggtc agacgccggg tcagttatcc aaacagaacg aggaccagag gatgagtaaa
     5941 gtcgtacgca ttattttcga atacaaggag cacgttatcc ataaaaacgc tgatggaaca
     6001 gtgcgcatgg gggtaagtct ggacatacgt tcaaccggga taaagcagaa aggtgatgga
     6061 cccgccatga tttttggggt ggttatgctc gcggaaagca gagactttgc tgaacttgtg
     6121 gcaatgaaag ccagtgcgct catgaaagat atgagcatgc gttccggggt tattaaaggt
     6181 aatgaattta atcagcaggg gtaattccat gagcaaagta cgcgttattt ttgaatttaa
     6241 gcatgtttcg catgacgaaa aaccggcagg caatgactgt gttgaagtgc atgaaaagat
     6301 tggtgtggat gtaaaaacag aacgtgatac gaataacagg ccgacgtcac tctgtggcgt
     6361 ttatgcaagt attctccagt atcacagtcc tgaaattatt cagtttctct cagcggaatt
     6421 tcaggcatct gtacaggctt ttggggcgga tgccatcatt aaacgccacc gcgtgcataa
     6481 agcatcaggc acactgcaat aaggaaaaac aaaatggcta aacgtgttac aaaattaaag
     6541 gccgcagccg aggctgcacc gcagacccgc gaagaagtca gccgcgatat ccgtaccctg
     6601 ggcgatattc agcgagaggc gctacggctg gaaacggcga tgaatgatga agtggcagaa
     6661 atcaccgccc gttatacgcc gcagattgaa aaccttaaaa aagaaatcaa agtgcttttt
     6721 aaggggattc aggactggtg caaaaccaac cgcgatgagc tgacgaacgg cggcaaaacc
     6781 aaaacagcca atctgaccac cggaacagtg tcatggcggc tgggaaatcc atcatgcagc
     6841 gtcagccgtg atgtggaagg tgtgattgaa atgctgcgcc gtatggggct tgagcgattc
     6901 atccgcacga aagaggaagt gaacaaggaa gcggtcctag cggagccgga tgcagtgaaa
     6961 ggcattgccg gtattaaggt gaataaaggc gctgaaagtt tttatgtcga gccttttgaa
     7021 caggacgccg gactgaataa ataacaccgc attaaatctt taaatatcac atcgttttaa
     7081 gtatggcgct cgcgtcaggg gactgcctgc gcctgtaaac tgaaaataag gaacaggata
     7141 tatggcatat ttttatttca aattagaccg tgtgcagaca aataaatatt tcaccaaata
     7201 tcaacagact gttttaccgc tacgcaacag tattcttcgg gcattactga aaaatacagg
     7261 cgctgccgga ttgcgcttaa agccgtttgc catggacgta atcagtgagt tttatttttc
     7321 tggtgctctg cctgcgggct ggcgtaagcg cgatgatgtg gcttttatcg gggacggacc
     7381 gtgctttatt gccagacctg atgagtcatg ccctgaagga ccggcgattg ccgcaatgat
     7441 tgaagccgct gaacgcgagt taagaaagcg tcctgatttc cttgtctggc tctgtgaaaa
     7501 gctgggggta atgagaatcc cctccatgtt taacatggac tcctggtgga ccccgtcgct
     7561 ctcccgtgat gccctgtgcg tggtgtttaa agtaggcgct tatggcaggg aaataaaagg
     7621 gggtattcct gaagaatgcc aggagattaa acattctgaa tatgtggcgc tgacggagga
     7681 ataattcatg attggtgcaa aagtgcttga aggggttaaa aactggctga gtatttacgg
     7741 gcgtctgacc tgcggcattc tggctgaaaa aatgaatatg ccgccatcct cgatggttta
     7801 ttttctgcgt gatgcggtcg atgccggggt gctgacggaa tgtaacggtt tttatgatat
     7861 tccgcgtccc cgcccggtgc agccggttcg tcgcaaatgc agccaggaag gtgcggctga
     7921 tgatgttcag tggtgcagct tcagaaaatc cctgccgtgg attgaggggc atgatattcc
     7981 gtcaatggcg tgggaatttg ctcagggcgt tctgacctgt gaaaccgttt atgtggtggc
     8041 tgaagttgat gagcaggcca tgaaagaagg cgtgccccag tttgtgatgg cgtatatcga
     8101 catacgcctg ggtgtcatta tctgcggttt aagcggctgg aatatcacct cgcgcgttct
     8161 gcgttacctg attgttgacc ggacggccgc gcctgccggg atatctgcgg aggtggcgta
     8221 atgttcttta aaacatcaaa cccttccgcg ctggccgcgt ggcaaaaata tcagcaggac
     8281 tgccagaaag ttaaggatga ggcaaaacgc cttgaggccg tgctgaatgt tgcgtgccgg
     8341 tcggtatttg tatccggtat cagtggcttt tgttttaaag ggctgcgctt tatggatgac
     8401 aaatatcctt ttcatcgcga cttatggcga aaaccgactg cgtcgaatgg ctggagctgc
     8461 acaccgcgca catcacgtat tcccaaagcc ctgcgcgttg cctctgacga acttaacagt
     8521 ctgtggcgtg aatattcgcc cgtcacgtat gccagaaccg atgcactgtt gttctggctg
     8581 ggtattgatt tctcggcaat catgtttggc cctgtgaagt ggttctgcgt tgacgatgtg
     8641 atttaccttg agtgcggcgt aaaacccgca aatcagaaaa tgaccgaaat tctgtctgat
     8701 gagttttatg ctgctgaaaa gcgagtcagg gggtgatgca tgatgatatt acaacccatg
     8761 gggagaaaag gccgtgcacc cgctcatgtt cgcgcatgga cacctgaaga agatgcgctg
     8821 ctgattgcgc tttatccatc caccccggtt aaggatattg ctgtcaggat aaaaagaagt
     8881 ttctggggtg tacataaccg gattgtttta ttacgcggta cttacccgga gttgctcaaa
     8941 tgcaaacgcc tcagatttaa acctgatgaa gataaattta tccgaaaaaa tgccagaaca
     9001 atgaccggta agcaaatagg agaatatctg gggcgagacc gggattctgt ccacaatcgg
     9061 gcgcgatata ttggcgtaag catgaaaaaa tacggagagt tgctgccctt cacccgcata
     9121 cctgacgatg atgttcgtct tattcgtgaa ttacgggatg ctgaatcacc acggcgtctt
     9181 acctttaggg aaatcggcga gaaatttgaa ttatccgagg gcacggtgag ttttatttat
     9241 caccgtcgtc ggactgccga agatgttgta ttacgggaat taatgccatg ataacgacat
     9301 tatttgttga atcagatgaa ccccttgtgt gtgccgtcgg aatgccggtc tgtggcggaa
     9361 cgctgaccgg tgtttatttc ggggatttgc gcggttatcc ctggcattca ctgaatgatg
     9421 ctttcccgcc tgatatggag gctgtcgtgc tgattgttca gtatggtgac caacaggaac
     9481 tgcgcatcgg ccatatgggg tatgaaggct tttttattga tgaagaaacc ggagcctgcc
     9541 ttgaagatga ggacggacag gtgacgcact ggtgccatat ttcagcttta ccggaatgac
     9601 gggaggtgaa tgcgtgagaa gttatattga taatgaaaaa ctgaagacaa tcagcgactg
     9661 tcttagcttg ctggcaaaaa taaaagaaac cattgaagag attaaatttc agcttgaata
     9721 cgaaccctgt ggcgatgata cattgcgtaa ctctgccaga aaagcactgg ctgtctttca
     9781 gaaacaacgc cggactgttg aataccgcct tgctgtattg cgccaggagg aaaaggaaag
     9841 aaatataaga cgtcatgaac tcgttaatga ttttcttgtt cgtgaattaa aggagcgtgt
     9901 gccggagtcg gtattttttg agtgtgaggc tgttgcccgt agcaaagcac tggaaactga
     9961 ttaaacaggc aggtgaatga tgagtattga attaagatcg tcttatgaat atcgtaaaat
    10021 tctcatcgcc ggaggcatga aaccggaaga tgcagaaaaa atcgtttctt ttatggataa
    10081 agaatgtgac aaacgggata tgccagaaat tattatggat gacatgattc tgcattcagc
    10141 cgtagcgtta agcccattat ggattgtgca ttctcttgct gaaattgcca aaggcaccga
    10201 taaacaggca gctgttgctg cccttcagac gcttaatgaa atgcgtatat ctccacgccc
    10261 gacattaata catatgattc tgtccagcat ggaggataaa gcaaatgaat agtctacccg
    10321 ctggatgggc gcggccacta atggccagga agcatcattt ttttaaaaca ggcgaaaata
    10381 tcagtatatg tggacggtgg ttatatctgg ctcataatcg cgagccggat acatttgaaa
    10441 gccctgatga ctgtgccgaa tgccgcagaa gagtgaataa ggagaaagat aatggacaat
    10501 agttctctgt tgttctggtg cttatatatc acatcctttt tcggcgcatt tgttattacc
    10561 cggtggttat gtcgaaaaat catctgcttt tttgataaaa gacatccggt tgaacgggcg
    10621 gctgatgcgc tcattcagca ggccattgtg ttatataacg gtgaattttt ctgccgaata
    10681 acaagcagag atggctggca cataatgatt attccgccaa cacaccatgc ccggtgggat
    10741 gaggcagaaa aggctttcca tgtccgtaaa aaggtaaata cggtatgaga ggaaaactga
    10801 tatccgccat tcatgtggca aagcgcgagc ttgccctgga tgatgagact tatacatccg
    10861 cgctgctggc agctaccggc aaaaccagct gccgggatat gtcaccggat gagttatccc
    10921 gcgtgctgga tgttttcaaa aaacgcggtt ttaaagtgcg tcagaacccg gttaaccggg
    10981 ccttaaaacc gggtacggtg accgccaaaa ttcgcgccat ctggaaggtg atgcaccggc
    11041 agggctttat caccgatggt gcggaaaccg cccttaaccg ctgggtgaaa tcgcagacgg
    11101 ccgcgcagaa cggcggcgaa ggtgtggcaa actggcagtg gctggagcaa caccccgccc
    11161 tggcctcaga tgtgctggag cgtctcaagc gatggcaccg ccgcaaaatg ctggccgcga
    11221 tgggaatgcc cgaacgcacg ctgatggggt atgacgccgt ttgcaggcag tatgaaaaat
    11281 cacttccccg ttaactccaa atcccgccac aacgcgggat ttttatttta aacttaccgg
    11341 gaacgcgaga accggaggct gatatggcag aaactcagat gagcatgttt ggtggtgaca
    11401 gtgagcaact gcacgccctt atcgaccgcc ttgatgacat ccctgatgat gttctcaaaa
    11461 agaactggcc gcggaccctg tcagaactgg ttgaagtcac cggcgcagaa cttcagcgtc
    11521 aggggattga gccggtactg gccggtaaac tggcacgcaa ggtggctgcg gctcaggccg
    11581 cctatatggg cggacggggt tattacctgc cggtcgggga atctctcttt gccgagctgc
    11641 gaaacaatga gatattttcg cgctgggacc ggggcgagaa aattgaatcc cttcgccgcc
    11701 attaccggat gtcagaaacc cagatttata ccgtcatacg cgaacagcgc cgtctgcatc
    11761 tggtaagaac gcaaccgcca cttttctgat attccgcaaa gcctgccgcc tctccttcac
    11821 cgttacgctg actcagagaa catcatgaga cagcgtaaca atgccaaaac tccccgcacc
    11881 actgcgtaaa aagctgattg cccttgttct ggccggtgcc gggacgttca cgattgccac
    11941 gcattacacc ggttactggg aagggaaaga aaactccacg tatatcgatc ccaccggtac
    12001 acccaccatc tgttacggcc ataccggccc ggatgtgaaa ccgggtatga ccctgacaga
    12061 tgaagaatgc ctggaactac tggaaaagga catgaaatgg gcctttgcgg ccattgatcg
    12121 gcgtgttcag gtgccgctta cccgtggtca gacggtggcg ctggcttcgt ggatcttctg
    12181 ggccggtgaa acgaactttc gcaactccac gcttctgcgc ctgatcaatg ccgggcagat
    12241 gcccgcgtcc tgtaagcagt atatccgctg gatttattca aagggggtga aactccccgg
    12301 ccttgaggcc cgccgttcgg cggatgaatg gttatgtcgc tacgacttgc cgaaagtctg
    12361 aaccgcttct ggcgaccgct catgatagcg ctgctgtgtg cggtactgct gttacggggt
    12421 gtcctgtggc tgcggtgaat aatgccttac cggccctgat tgccggggcg tgcatggcgg
    12481 cgctggggat tatcgctgta ctggttgccg ctatatgggg aatgcaccag aagacgcagc
    12541 gccttgaaga caataatcag gtgcttgtgc gtgagcggga tgaggcgcgt caggtgctgg
    12601 caaatcagca gcacaccctg caactcattt cacaaatcag tgaggcggcc acgaatgaaa
    12661 aacaacagaa cattcagcac agcgaggggc agcagagcgt tgtccgccgg tcgctggagg
    12721 cagtgcctgc ggcctctgcc cctgttcctg atgatgtggc tgatcgggtg cgccgggccg
    12781 tctgtgaaat acgtgcctgt gaagccggtg ccgatccccg ctgaatggct ggctgactgc
    12841 ctggtccctc ctgcgccgga gccgttcacg tttggggcat cggtcactta caacctgcaa
    12901 ctgctggcgg tgatcaagaa ctgcaacgtg gacaaggcca gtattcgtcg tctggaggcg
    12961 cggcgacaac atgaatttac tgatatggcc ggaacgcctg ctgttccggc aggaaagacg
    13021 aagtaaggaa aaaggtatgg atgattcaga tcgtgctcag gctgtgatgg agcggggatc
    13081 agaacgcgcc ctttgtaacc ggctgacacg taagcgccgg gcaatggatg ctccgggcag
    13141 acgtgtctgc gcggactgtg gcggagagat ccccgccgca cgtcttgttg ctgtgccgga
    13201 tgccatccgt tgtgtgaact gccagaacat catggaggcc cgtcatgtgg gtcagcatcg
    13261 ttaaagatta tgttgtgccg atcctttcgg cgacggcaac cgctggcggg atattcatgg
    13321 cgctgatgcg caaaacgttt gtcccccgcg aggcctttga aaaactctcc gaccgcgttg
    13381 agaaggtgga aacccgcctg tcgtcactgc cgacagaagc cgaggtcaac cgtctgaatg
    13441 tggaaatcgt gaccctgcgg ggtgaactga aaaccacgaa cgccacgctc cgctctgtct
    13501 cctatcagaa cgaactgctg ctggagcagg cggtaaggaa aaaaacacaa tgagtgattt
    13561 cattactgaa gatcagcgtc ttgtcattct gcggtcgctg gcggattaca acggcgaact
    13621 gggtgaatcc gtgctccagg actgtctgga tgattacggt caccgcgtgt cccgcgacac
    13681 cgttcatact catattgcct ggcttgccga gcaggggctg gtgcgtaagc gcacgctcgt
    13741 taatggttat ttcatcgctg aactgaccgg ccggggtcag gatgtggcgg aaggccgcgc
    13801 caccgttccg ggtgtgaaga aaccacgcgc aagggggtag cgatggacaa gccgacgcgt
    13861 ggccgcgtgc gcaaggtgga tttgctcccc gacagcatcc gtaagccact gctggaaatg
    13921 ctgcgtgaaa aacgtctgac gcaggtccag atccgcgagg aaatcaaccg tctgatccgc
    13981 gaggcaggcc tgccggaaga gcagcagctt tcaccggcgg ccatcagccg ggaagcctcc
    14041 cgtaatgagc tgattgcccg taacctgcgt gatttacgcg agcagacaaa agccatgatg
    14101 gccgaactgg gggacaagcc gaccggtgaa accacggcac tgattctgga gatgtcccgc
    14161 gccctgatgt accgccgtct gcgtgccgcg accgaatccc tgaacagtga cagcgacgtg
    14221 gatatgcgtc ttattaagga catcctcctg tcagcacagc gtgcggaaag tgccgccgag
    14281 cgcagcatta aacgtgaaaa agaaatccgg gctgcatttg ctgaagagat ggcaaacgcg
    14341 gttaccgacg agctgcgcgg cgtggacggg atgagtgaac agcttgagtc ccgtattaag
    14401 ggaattctgt tgggtaaagc ctgatgaata acgccgaaga actctccccc ttactgacga
    14461 ataccgtctc cacccgcaaa attgatctgg ccggtgaaaa agccctgctg ggcgtggatg
    14521 tgccggacag ccttgatttg cccggtgata tgccggtgtt tctggattat caggcccgct
    14581 ggtttgagga tgaaagtgag gtctgcatcg cggaaaaatc ccgccgcacc gggcttacct
    14641 gggcggaagc cgggcgtaac gtcattaccg ccgcaaagcc taagcgtcgt ggtggccgta
    14701 atgtgtttta tgtggggtca aagcaggaga tggcgctgga atatatttct gcctgcgcac
    14761 tgttctcccg tgcctttaac cagctggccg atgctgatgt gtatgagcag accttctggg
    14821 atcgggataa aaaagaagaa attctgacct acatgatccg ctttccgaac agtggattca
    14881 aaattcaggc actgtcttcc cgtccgtcaa acctgcgcgg ccttcagggg gatgtggtga
    14941 ttgatgaagc cgcgttccac gagtcactgg atgagcttct caaggcggca atggcgctca
    15001 ccatgtgggg cgcgcgcgtg cgtattatct ccacgcataa cggcgtcgat aatctgttta
    15061 atcagtacat tcaggaagcc cgcgagggac gcaaggatta cagtgtccac cgcataaccc
    15121 tggatgatgc cattgcggac gggctgtacc gtcgtatctg ttatgtcacc ggccgcgaat
    15181 ggtcaccgga aagcgagcag aagtggcgtg atgatctcta caaaaacgcc ccgacccgtg
    15241 aggatgccga cgaggaatac ggctgtatcc cgaaaaaatc cggcggtgcc tatattcctc
    15301 acgcgcttat tgaaatggcg atgatccgcg acatcccgat tctgacgttt gaagccccgg
    15361 acgacttcat cagccgtgcc gcatggctgc gtgaatcgga ggttttaacc tggtgtgaag
    15421 aacatttaaa accgctttta gaggcgttaa atccccgttc ccgcttcagc ttcggggaag
    15481 actttgcccg caccggtgac ctctcctgct ttgtgctgct ggaaatcact gaatccctgg
    15541 caaaacgcga ggtgtttcgc gtggagctgc gcaacctgcc gtatgcccag caggagcagg
    15601 tgatgatgta catcctgacc cgcgttccgg cgctggtcgg tgcggcgttc gacgccaccg
    15661 gtaacggcgg ctatctggcc gaagccgcgc tgctggcctt tggcccggac atcatcgact
    15721 gcgtgatgtt atcgccgaag tggtacggcg agtggatgcc aaaactgaag gccgagtttg
    15781 aggatcagaa tatcctcgtc gcccgccatc agaccacgct tgatgatttg cgccatgtga
    15841 aggtggtgaa cggtatcccg caaatcgaca aggggcgtac gaaggaccag aacgcaacag
    15901 ctgtgaacgc ccgccgccat ggtgattttg ccgtggcgct ctgtatggca aaccgggcgt
    15961 catacatgga gggctttatc ctggatgaat cagcctgcca ggcgctgccg gagcggtcgc
    16021 gggccatgga gggcggttat cgtgatgacg atgaggcata tcatgaattt gatcgggggt
    16081 gctggtagtg ggacgcataa ttgatcttga cggaaaacct ttctcctttg acccggagat
    16141 gcagagtgcc gtgctggata ttccgcagat tgccagccgt tatattgaac atccggcctc
    16201 gggtatcacc ccgaaccggg cggcacagtg ccttcgcggg gctgaacgtg gcgatctgat
    16261 tgcccagtcc gatctggccg ctgacattga agaaaaggat acccatcttt ttgcagagct
    16321 gggcaagcga cgtcttgcca ttcagggggt gccctggagt attgagccgc ccccgaacgc
    16381 cagcgcgaat gagaaaaagg acgcggaaat gctcgacgaa tacctgcatt ccgccgactg
    16441 gtttgatgcc atgctgtttg atgccaccga tgccatcctg aaaggctatt cctgcatgga
    16501 gattgagcac gggatgctcg gtaaaatgca catcatccgc gccatccgct ggcgtgacag
    16561 cgggcatttc tgccttaacc cggatgattt gagcgaactg cggctgcgtg acggcagcca
    16621 tgccggggtg gcgtttcagc cctttggctg gatagtgcat cagtcgcgtt cacgcactgg
    16681 ctacggtggt gcgacggggc ttgtcagaac gcttatctgg ccgttcattt tcaaaaacta
    16741 ttccgtgcgc gatctggctg aatttctgga ggtgtacggc ctgccgatga aggtcggtaa
    16801 atacccgtcc ggggcaacac cggagcagaa aagcgccctg atgcgggcgg tgatggatat
    16861 cgggcgacgt acaggcggga tcatcccggc cgggatgtcg ctggagtttc aggcggcagc
    16921 gaacggtcag gccgatccgt ttgaaaccat gatttcgtgg ggggagcgtt ccatctccaa
    16981 agctatcctc ggcggcacgc tgaccacgga agccggagac aagggcgcac gttcgctggg
    17041 tgaagtgcat aacgaggtgc gccgggaaat cagggattct gatttacgtc agctggccgc
    17101 cacgctgaac cgcgatctgg tgtatccgct gtatgccctg aacaccgccc acgccattga
    17161 tatccgtcgc ctgccgcgta tctgtttcca gacaaaagag ccgggggata tcaccaaaat
    17221 caccagtgcg gtgatgcagc tcagtacggg gatggatatt cctgatccct gggtgcggga
    17281 gcagaccggt attccacagc ctgcccccgg tgaagccatc ttccgtgtcc gtcagagtgg
    17341 caatgaacct gctcagacag acaaagagat gccaccggaa aaacaggaga agacagagca
    17401 gacagcgctg gctgccagac tgccggaagc gaagagcagc ccccgagatg aactggacga
    17461 catgggggat gcggtgcctg cccgccggtt acaggaggcc atcgacccgg tactggagcc
    17521 ggtcattgaa gccatcagaa cgcgggggct ggcggaggca ctggcagacc tgcctgccct
    17581 ttaccgtgaa atggatgatt cccgcctgat gacgctgctc agtgatgcca tgtttgctgc
    17641 ggaaatgaag gggatgctgg atggcacagg ggattgattt aggttatgcc gccacgcttc
    17701 cctcaaaaga ggcggtggca tacttccgcg ccaaaggggc gcatatcagc tggaactggt
    17761 tcgaaacaga cgcggatgtt catgcccgtt cattcacggc ggcaaaagtg gcacgcctgg
    17821 acgtactgac cacactacag gcggaagtgc agcgtgctgt tgatgagggg atttcacaga
    17881 aagcatttat ccgcacactg acaccccgcc tgcaaaagct gggatggtgg ggaaagcaga
    17941 ttgtggtgga cagcgccggt aacgcagaag aagtgcaact gggcagtccc cgccgtctgg
    18001 cgctgattta caacgtgaac acccgcgtgg cttacaatgc cgggcgttac acgcagatga
    18061 tgaacaacac ggacacgcat ccgttctggc agtatgtggc ggtgatggac agccgcaccc
    18121 gcccggcgca ttccgccctt aacgggcagg tattccgcta tgacgatccg ttctggaata
    18181 cacactaccc acccaatggc tggaactgcc gctgccgtgt ccggccattg tctcagaccc
    18241 gtctggatgc aatggggtta tccgtttcat ccggtcagga tcatctttcc acccgcaatg
    18301 ttgaggctgg cgtggataag cagaccggag aagtcagaga aatgccggtg accacatatt
    18361 cagatggcac cagaaccatg acaccggatg tgggctggtc atataacccc ggctcggcgg
    18421 cgttcggcac agaccaggcg ttgatccgta aactgatcga ggtgaaaagc ccggcgctgc
    18481 gggaaatggt agtgcaggag atgaacaaca gcccggagcg gcagctggcg ttccgcatct
    18541 gggcaaaaaa catcatgaaa acccggcgag gcggtaacga tatccgcacg ctgggcttta
    18601 tgaccgaaag cattgcgcag gcggtggaaa gccggacggg aacgccaccg gcccgcctgc
    18661 tggcgatgag cggtaaaaat gtgctccatg cggacagcat gaaacatcag aatgacggta
    18721 tcgccctgac gccggaggat ttcggtcgat taccggcaat gctggcaaag ccaaaagcgg
    18781 tgctgtggga caaacgccac aacaatctga tgtatatcgt ggaaagtaag gatagcagtg
    18841 tgcagattgc cgttaatgcg ccgtacagcc tgaagcgtca gccggataaa ctggatgtga
    18901 ttgtgaatgc ttaccgggtt atcaatatgg ataagctgaa atcggatatc cggggtggaa
    18961 tgcttgaggt actggaagga gatattgatt aacggtcagt ggcgggggtt gaacccgcat
    19021 acatgatcca cccggaggca ggcaccgact ttaccagtta agcgtacact gaccgcctgt
    19081 ttattttata ttcatccttt atgcggaggc aatatgtcat ccattgatgc cgctgtcgtt
    19141 gttgacgtta cgcgtctcca gcgggtcttt gcccggcttc agtttgtggg tggagggaaa
    19201 gacctggccc gcagtgtggc ggcgagtctt ctgtcatcgt cagaaatggc gtttgaacag
    19261 gaaaaagagc cggacggcga acgctggcat gactggtcag atccttaccg caagtggcgt
    19321 acccgtaagg gatacatgcc cggcaaaatc ctgacgctga acggcgatct ggcccgacgc
    19381 ctgaccacgg attatggcga tacctgggcg ctgattggat caaatgagcc ttatgcggcc
    19441 attcatcagt ggggtggcct gcctggtatg ccaccgggac cggcggccat tggcgcacgc
    19501 ccgtatatgg gctttgatca ggtggcagag caggagatca tggacgaaat cagaaaacgc
    19561 tttaaaaagg ccacagaaac gccttaattg tttaaggtat gcaaatgcat taccttaccc
    19621 ccttcaggcg cgtgtcgtga ttttctaacc tgtatttaac gggctttaaa atctgcgtga
    19681 agcgcctttg tatttttccc ctggaagcac ttcctgaaat cccgcaaaac ccgccctgaa
    19741 aattcccggc ttatgctgcc ggaatgaaga cgaaaaacac gcccaaactt gcttatgcca
    19801 ttctgaatgc catcagcctg tccgcagacg gggacggtga ctggtgtcag atcatgccag
    19861 ccggtcgggt aaaggcccgt gacggtcgcc cggaaaaacc ggccgagggc tggctgatta
    19921 accgtgcgac cgttgagcgc atggtatccc gggttgtggc gctcaatcag ccggtgaaaa
    19981 ttgactacaa ccaccagacc ctgattaagg ggcatcaggc accggctgcc ggttttgtga
    20041 tggcctcacc ggagaatttt cgcttcagtg aagagcgtgg cttcgaggtg cgcccgaagt
    20101 ggaaccctcc ggcccttgaa catctgcgta ataacgaatt cccctggttt tcaccggtga
    20161 ttggctatga cgagaacacc ggcgaacctg tcgaacttcg gatgctggct attaccggtg
    20221 accccggtct gaccggcatg aatcctgtcg ccgcgctgtc ggcggatgac ctttataacg
    20281 ccttaaaccc tcctttaaag gatacctcca tgaatgagca attacgccag ttgctgacgg
    20341 cgctcggtct gaccgtggct gacggtgacg aatttacgcc ggagctgggc acggcggcgc
    20401 tgtctgccct caccgggatc aaaacccgtg cggatgcaca cgacaacctg aaaacacagg
    20461 tcgccagtct gtcggcagag ctggaaaccg caaaaggcac accggctggc ggcattattg
    20521 atctgacgaa atacgtgccc gttgaaacgt ataacgccct gcgtaccgaa tacgttgcgc
    20581 tgtcggcgca gcacggcagc accacgctgg agcaggtgct ggacaaggct gaatccgaag
    20641 ggcgcatctt caaaagcgaa cgcggctatc tggaacaact gggcgggcag attggcgttg
    20701 cggcactttc tgcacagctt gacgcccgcc agccggttgc ggccctgacc tctcttcaga
    20761 ccgacaccgt gaccgtgccg gataaaaaga ctgccaccgc tgcgctgtcg gctgaagata
    20821 tcgcggccgc caaactgctg ggtaaaaccg aagccgagtt cctgaaaatg aaagaggaaa
    20881 tgcaataatg cctaccccga ttacaccggc gatgatcaca gccctgatga cgggctaccg
    20941 ttctgatttt caggccggga tgtccatggc cccgtcgcag tataaaaaaa ttgcgatgac
    21001 cgtgccctcc acatcgaaat ccaacaccta cggctggctg gggcagttcc cgcagttccg
    21061 tgagtggatc ggctcccgcg tcatcgagaa gatgaaagcc tatggctatg ccatcgtgaa
    21121 caaaactttt gaaggcactg tcgccattag ccgcgatgac ttcgaagacg acaacctcgg
    21181 tatttattcc ccgttgttcc aggagatggg gcgcgctgcg gcggtacaac cggatgagct
    21241 ggtctttgct gccctgcgtg acgggatcag tgcggcctgc tatgacggtc agaacttctt
    21301 tgataccgaa catccggttt accccaaagt ggatggctcc ggggatgcgc agatggtcag
    21361 caatatgttt gtggcaaaaa ccggctctgt cggcgcacag gctgattaca gcggtccggc
    21421 ctggtatctg cttgactgct cccgcgcggt aaaaccgctg atttatcagg atcgccgtaa
    21481 ggctgaactg gttgcccaga ccaaagtcga tgaaggtcgc gcattcaccg ataacgaatt
    21541 tgtgtttggt gcatccgccc gtcgcaatgt gggctacggc ttctggcaga tggcctacat
    21601 gatgcagtca ccgctgacgc tggatgcact gtggcacggc tggtcagcca tgcgcgaatt
    21661 taccgctgac ggtggccgca aactcggcat taaacccacc catattgttg tccccacctc
    21721 gctggaaaaa caggcggtgc agctgctgga gcgtgaactg ttcgcagacg gaaacgcaac
    21781 cgtctctaac gagatgaagg gtaagctgga gctggttgtc gcggattatc tgtaaacggt
    21841 gagccgggct tacggcccgg cccctgcgga gggcaaatat gaatgaacat cacactgtgg
    21901 cagcaacaga tgattcaggt cttcaggtca gtggtgacaa ttctgtcacg gtgctggcag
    21961 aagtgcgttg cagtcgttcg gcgtttcgtc gtgcggggtt cctgttcacg cgtggacgtc
    22021 agcaggttga ggtcaccccg gagcagcttg cccgactgga ggaggagcca tgcctcactg
    22081 tgcgaatact acagacgtct gctgatgatg cggggagcgt ggcgggtgtg gttcatgcag
    22141 tggcc
//
LOCUS       DAZGJW010000072        21638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZGJW010000072 DAZGJW010000000
VERSION     DAZGJW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.072"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..307
                     /gene="cybC"
                     /locus_tag="ACHKIK_002806"
     CDS             <1..307
                     /gene="cybC"
                     /locus_tag="ACHKIK_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO9083949.1"
                     /translation="NMETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLE
                     DKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(352..816)
                     /gene="nrdG"
                     /locus_tag="ACHKIK_002807"
     CDS             complement(352..816)
                     /gene="nrdG"
                     /locus_tag="ACHKIK_002807"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO9083950.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(975..3113)
                     /gene="nrdD"
                     /locus_tag="ACHKIK_002808"
     CDS             complement(975..3113)
                     /gene="nrdD"
                     /locus_tag="ACHKIK_002808"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO9083951.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(3507..5162)
                     /gene="treC"
                     /locus_tag="ACHKIK_002809"
     CDS             complement(3507..5162)
                     /gene="treC"
                     /locus_tag="ACHKIK_002809"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO9083952.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(5212..6633)
                     /gene="treB"
                     /locus_tag="ACHKIK_002810"
     CDS             complement(5212..6633)
                     /gene="treB"
                     /locus_tag="ACHKIK_002810"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO9083953.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(6752..7702)
                     /gene="treR"
                     /locus_tag="ACHKIK_002811"
                     /pseudo
     CDS             complement(6752..7702)
                     /gene="treR"
                     /locus_tag="ACHKIK_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
     gene            7884..7937
                     /gene="mgtL"
                     /locus_tag="ACHKIK_002812"
     CDS             7884..7937
                     /gene="mgtL"
                     /locus_tag="ACHKIK_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO9083954.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8078..10774
                     /gene="mgtA"
                     /locus_tag="ACHKIK_002813"
     CDS             8078..10774
                     /gene="mgtA"
                     /locus_tag="ACHKIK_002813"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO9083955.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(10957..11310)
                     /gene="yjeI"
                     /locus_tag="ACHKIK_002814"
     CDS             complement(10957..11310)
                     /gene="yjeI"
                     /locus_tag="ACHKIK_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO9083956.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11448..13094)
                     /gene="groL"
                     /locus_tag="ACHKIK_002815"
     CDS             complement(11448..13094)
                     /gene="groL"
                     /locus_tag="ACHKIK_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO9083957.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13138..13431)
                     /gene="groS"
                     /locus_tag="ACHKIK_002816"
     CDS             complement(13138..13431)
                     /gene="groS"
                     /locus_tag="ACHKIK_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO9083958.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13707..14963
                     /gene="yjeH"
                     /locus_tag="ACHKIK_002817"
     CDS             13707..14963
                     /gene="yjeH"
                     /locus_tag="ACHKIK_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO9083959.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            complement(14979..15455)
                     /gene="fxsA"
                     /locus_tag="ACHKIK_002818"
     CDS             complement(14979..15455)
                     /gene="fxsA"
                     /locus_tag="ACHKIK_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO9083960.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            15792..17228
                     /gene="aspA"
                     /locus_tag="ACHKIK_002819"
     CDS             15792..17228
                     /gene="aspA"
                     /locus_tag="ACHKIK_002819"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO9083961.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17346..18647
                     /gene="dcuA"
                     /locus_tag="ACHKIK_002820"
     CDS             17346..18647
                     /gene="dcuA"
                     /locus_tag="ACHKIK_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO9083962.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            18763..19101
                     /gene="cutA"
                     /locus_tag="ACHKIK_002821"
     CDS             18763..19101
                     /gene="cutA"
                     /locus_tag="ACHKIK_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO9083963.1"
                     /translation="MLDEESSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            19077..20774
                     /gene="dsbD"
                     /locus_tag="ACHKIK_002822"
     CDS             19077..20774
                     /gene="dsbD"
                     /locus_tag="ACHKIK_002822"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO9083964.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEVKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            20811..21386
                     /gene="yjdC"
                     /locus_tag="ACHKIK_002823"
     CDS             20811..21386
                     /gene="yjdC"
                     /locus_tag="ACHKIK_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9083965.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21493..21568
                     /locus_tag="ACHKIK_002824"
     tRNA            21493..21568
                     /locus_tag="ACHKIK_002824"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21526..21528,aa:Phe,seq:gaa)
BASE COUNT         5046 a         5630 c         5578 g         5384 t
ORIGIN      
        1 caatatggaa accctcaacg acaatttaaa agtgatcgaa aaagcggata acgcggcgca
       61 agtcaaagac gcgttaacga agatgcgcgc cgcagcgctg gatgcgcaaa aagcaacgcc
      121 gccgaagctc gaagataaat caccggacag cccggaaatg aaagatttcc gccacggttt
      181 cgacattctg gtcggtcaga ttgacgacgc gctgaagctg gcaaatgaag gtaaagtaaa
      241 agaagcgcag gctgctgcag agcaactgaa aacgacccgc aacgcctatc accagaagta
      301 tcgttaattc ctcatttccc tgttgcctgc actcaggtaa cagggaactt atcatcgcaa
      361 atgatgcacc acctgattgc tgctgccgcg ccagatcagg gaagggtctt ttaaatcctg
      421 cacaaatttg ccgtcgacca gtacgttaat caaatcaaca acctgcattt gcgcagcgtt
      481 gagttcgtcg agtttatagc ctgtccacac ccagatgtct ttacccggac actcggcgcg
      541 gatgcgtttt accagtttca gaatatccgg cacgttttgc ggatgcagcg gatcgccgcc
      601 ggagagggaa ataccctggc gtttgatacg agtgtcattc agatcgttaa taatctggtc
      661 ttccattgct ttagtaaatg tctgaccgga atttacccgc catgtgcttt tgttatagca
      721 accggggcat tcatgaacac acccggagac aaacagggtg cagcgagtgc cggggccgtt
      781 gacgatgtcg acaggatagt actgatgata attcattgtt tcgcctggct tatcagtccg
      841 cgtataggat gcatccggta atcaacgcct gatgcgacgc tgtcgcgtct tatcagacct
      901 acaatcgttg ttgccatgta ggccggataa ggcgtttacg ccgcatccgg catttgctta
      961 acgtagagtg aagattaacc tatctgccca ttccccaaat gtttaacgcg gcgcttaact
     1021 tcttcctgct taccggcgtt aaatggacgt gcatccgggc tacctaaata tccgcacacg
     1081 cggcgagtca ccgacacacg ggaggcgtca tggttaccac atttcgggca agtgaagcct
     1141 ttgctggtgc actcgaactc accggtaaag ccacactcgt agcactcatc aatcggtgta
     1201 ttggtgccgt aatacggtac atgctgatag ctgtaatccc agacatcttc cagcgccttc
     1261 aggttgtgct gaatgtttgg atactcgccg tagcaaatga aaccaccgtt cgccagcggc
     1321 gggtaaggcg cttcaaagtc gatcttgtcg tacgggttca ccttcttctc cacatcgagg
     1381 tggaaactgt tggtgtagta acctttatcg gtcacgcccg gcaccacgcc aaactcagca
     1441 gtatcgagac ggcagaagcg gtcgcacagg ttttcactcg gcgtgctgta gagactgaaa
     1501 ccataacccg tttcttcttt ccactgatcc actgcctggc gcagacgttc aacaatggcg
     1561 atacctttcg cgcgaagctg ctcgttgtcg taaacatgct cgccgccgaa cagcgcgtta
     1621 atggtttcgt ggatgccgat gtaacccagc gaaatagacg cacgaccgtt tttgaagatt
     1681 tcagaaacat catcgtcagc attcagacgt acgccacaag caccttccat atagaggatc
     1741 ggggccacgc gcgctttcac gccttcgaga cgagcgatac gggtcatcag cgccttacgt
     1801 gccagcacca gacgttcatc cagcagcttc cagaaggtgg cttcatcgcc ttttgcttcc
     1861 agagcaatac gcggcaggtt caggctgatc acgccgaggt tgttacgacc atcgtggatc
     1921 tgctcgccgt tttcattttc ccacacgccg aggaagctgc ggcagcccat cggggtttta
     1981 aacgaaccgg tgactttcac tacctgatcg tagttcagga tatccggata catgcgcttg
     2041 cttgcgcact ccagcgccag ctgtttgatg tcgtagttcg gatcgccttt tttatggttc
     2101 agaccatcgc gaatcgcaaa caccagtttc gggaacaccg cagttttacg gtttttaccg
     2161 aggcctgcga tacggttacg caggatcgat tcctgaatca ggcgcgattc ccagctggtg
     2221 cccagaccaa aaccaaaggt tacaaacggc gtctgaccgt tggcggtgtg cagggtgttt
     2281 acttcgtact ccagcgactg gaaggcatcg tagcactctt tgatggtacg agagtttgcg
     2341 tagccttcgg cgtccgggat gttccactct tctgcggtct tgcgatgttt gttgtagctg
     2401 gcggtaacaa acggcgccag cacttcatcg atacggttaa tggtggtgcc gccataaata
     2461 tggctggcga cctgagcaat aatctgcgca gttaccgcgg ttgccgtaga gatcgacttc
     2521 ggcggttcaa tctcggcgtt ccccatctta aagccctggg tcagcatgcc tttcaggtcg
     2581 atcaacatgc agttgaacat cgggaagaac ggcgagtaat cgagatcgtg atagtgaata
     2641 tcgccacgct catgtgcctg caccacgtca cgcggcagca ggtgctgacg tgcatagtgt
     2701 ttagccacga tcccggccag caggtcgcgc tgggttggaa tcaccttgct gtctttgttg
     2761 gcgttttcgt tgagtaacga ggcgttggtc tgctcgacca gaccacggat ctcctggttc
     2821 aggcgaccgc gtttttcacg ttcaatgtcg cgatcgtgac ggtactcgat gtaagcacga
     2881 gccagttgtt tgtatggacc cgacatcagc tggttttcaa ctgcggtctg gatctcgttg
     2941 atatccacct ggttgcggcc ctgcatctgc tcgctgacaa ccgcggcaac agtggcgcaa
     3001 taatcggcat catcgacttc cgctgcttta gctgcacgca gaatcgcttc tttgatgcgc
     3061 tctgatttaa acggcacttt gcagccgtct cgtttcatca catgcggtgt catgatcgct
     3121 ccatattttt aagaacaggt tatccacaga aattgggaaa ggcattttcc ggttttttcg
     3181 tttcgttttc cggtgctttc cgcaatcgcc atccgcttat ccacaacgcc gcaccttttt
     3241 cgtggggagc tgtagtagca attatagtcg attaatacaa catattgggt tgggacgcat
     3301 tttaaagtct atatatagtg ctttgcatca aggatgtttt aacttttttt gatgtagctc
     3361 aaagtaaaaa gcagagcgta cggatgacgg gcgctacagc gttatgtaaa ttttttaatg
     3421 aatttgctgg ttgaaaaatc aacaaaaaca acatgctgac agacaaaacc ccggaatgac
     3481 cggcattacc ggggcttagg gaagatttac ttctgtaacc accagacagc ctcaaaaggc
     3541 cgtaaattca tggcacaggg ttgtggtgag gcttcttcgt agttatgcat cacaagctgc
     3601 cagttgccgc gcatttgccc tggctgccag ggttggatct cacggctaag gttggcaatg
     3661 accagcaagg tttgcccctt ccattcacgg cgatagcacc acaatacagg gctgtttggc
     3721 agcagatcct ggtaattgcc ccatgtcagg atggcttctt gcttacgcag tgcgattaac
     3781 ttttggtagg tgtaaaacac cgaggagtca tcggccagcg cggcttctac gttgatttgt
     3841 tgatagttat cgcccaggcc aatccacggt tcgccagccg taaacccggc attatcgccg
     3901 ttgctccatt gcatgggcgt gcggctgttg tcacgggatt tactggcgag gattgccaat
     3961 aactcgtcgg catcacgacc atcgttgcgc agctcggcaa acatattgag gctctccacg
     4021 tcgcgatagt cagtaatgcg cgtgaaatgc gggttggtca tgccaatctc ttcgccttgg
     4081 tagatatacg gcgttccctg catgccatgc agcaccatcg ccagcatttt tgccgcaggc
     4141 acgcggtatt caccttcatc accaaagcga gaaacaatgc gcggctgatc gtggttacac
     4201 cagaacaagg cattccatgc cacgttgtgc attccttgtt gccagtggcg gaacaatgtt
     4261 ttcaacgcca caaagtcagg tttagccagc gtccattttt caccaccggg ataatcgacc
     4321 ttcaggtgat gaaaattaaa ggtcatcgac aattcactgc ctgtcagtgc cgcgtatcgc
     4381 tggcaatgct caaggctggt ggaggacatt tcacctacgg tcattaaccc gcgtggcgta
     4441 aacacatcgc ggttcatctc gtgcaaaaac tcgtgtgctc gtggcccgtc ggtgtagaag
     4501 cgacgcccgt cgccatccag gtcttcaggg aaacgcgggt ctttggagat cagattcacc
     4561 acatccaggc gcaacccgtc gaccccacga tcggcccaga attcacagac ttttttcagc
     4621 tctgcgcgta ccgctggatt ctcccagttg agatccgcct gttctggtgc aaagagatgc
     4681 aaatagtact gttcgctttc cgcatgccag cgccacgcac taccgccaaa ttttgaacgc
     4741 cagttgttcg gtggcgtttc tggttcgcca tcgcgccaga tataaaactg gcggtaaggg
     4801 ctttctttgt tcagcgcctc gcgaaaccag gcatgttggg tagaggtatg gttaaacacc
     4861 atatcgagaa tgatacgaat cccgcgcgat tttgcctgcg tcaccagttc gtcaaaatcg
     4921 tccagcgtgc cgtagatggg atcaatcgcc gtatagttcg ctacgtcgta accgttatcg
     4981 acctggggag agatataaaa gggggttagc cagatggcat caacgcccag tttatgcaga
     5041 tagtccaggc gttggataac gccacgtaaa tcgccggtgc cgctacccgt ggtgtcctga
     5101 aaactctttg gataaatctg gtagataacg ccgttttgcc accagtgggg aagattagtc
     5161 attacgttat tcctgcaaat gcgagggggc gcaattgcgc cccgaagaaa attaaacaat
     5221 atccagcgtg cccaggcggt atttccgctg atagataaac gaggtgagta caatcgggat
     5281 gatgatggcg ataaccattg ccagcgcaaa tacctgccag tagctcggtt gaatcgagag
     5341 aattcccggc aggccgccta cgccgatacc attcgccata acgccgttca ggccgcatag
     5401 taatcctgcc agaccagaac caatcatcgc gcacagcatc gggaagcgat atttcaggtt
     5461 gatgccgtac attgcaggct cagtgacccc aagccaggcg gagatagcgg caggcacgga
     5521 gatctcgcgt tcattgtgct tgcggctgga aatgataatg cctatcacgg cggagccctg
     5581 agcgatattc gacagcgcaa tcagcggcca cactggcgtg ccacccatgc tttgaatcat
     5641 ctgcaaatca atagcaaggg tggtctggtg tacaccggtg atcaccagcg gggcgtacag
     5701 gaagccaaac aatgctgcgc caatcggagc aaagctgccg gtcatcaggt gacgtaccgc
     5761 aaaggcaacg ccatcgccaa tcatgcgacc aaacggacca atcagcgcat gggcgaggaa
     5821 caccgcgagg atcagcgaac agacgggcac caccaccaga tagaggtaat ccggcacgat
     5881 gcgtttaagg cgagtttcaa taacgcccag cgccagcccg gctaacagtg ccgggatcac
     5941 ctgcgcctga tagcctactt tggcgatgct gaacatgcca aagtcccaca cttccggcag
     6001 ctgctgcccg agcagataag cgttcatcag ctggggagaa accagtgtca cgccaagcac
     6061 gataccaagg atcggcgtgc cgcccatttt tttcaccgct gaccagcaaa taccgaccgg
     6121 caggtagaag aagatcgctt caccgatcaa ccacagaaaa tcgtagatcg tttgcaggga
     6181 agggtacatt tgcgccagcg tctgaccgtt gctcatgggc aaatcgccga tcacattgcg
     6241 aaaaccgagg atcaaaccgc cgctaatcaa cgcgggcagc agcgggaaga agatctccgc
     6301 gaaatgagag atcaactgct catgccattt catattctgc cgggcggctt tttttacctg
     6361 ctctttatca acctgcgcct gtccggttga cgcaatcagt gcttgatagt aatcacccac
     6421 attggtgcca atcaccacct gaaattgccc ggcattggtg aaacagcctt tcaccatggg
     6481 gagttgctca atttctttcg gtctggcatt ggccggttgg ttgaggacaa agcgcaggcg
     6541 agtaatacag tggctcaccg tcgcaatatt gccgcgcccg ccgaccagtt caatcaaccg
     6601 atcgatatcc gtttggttta ttttgctcat cataaagccc catggcagat gacatttttg
     6661 gttggctgca gaatatattg cgcggaaaaa tttaaaaacg ggaacgttcc cgaaacacag
     6721 cgaagatcac aatttatcgt tcaggaaacg atcaggacag ggtggcgggg atgatgattt
     6781 gttgcggttc gctgcgcccg gttacctgtg cgatcaattg gcaggccgcc tggcgtccag
     6841 cttcggcgta accaggatct acggtgacga tctccggatg aaggaatttc attaacggcg
     6901 tattaccgac gctcgccagt tgcaaggtgt cgatgcgttg ctcttgcagg tatttacttg
     6961 cgccaagtgc cagcgtgtcg gttgcgcaca gtaaggcggt agtttcaggc gtaatcactt
     7021 ttgctacgtt ctcatagcct tgcttcatag caagccctgg cagagcggca acgggatgca
     7081 gtttatgcgc tttgcagaac gccaggtagg cttcgtgacg tcgcttaccg gtcgtcacgt
     7141 cactgtgcgg cacgccgaga taactgatat tacgatgccc ctggtcatag agccgttgca
     7201 tcaggatctt gattgcccct tcgtcgtcat aacagaccga agcaaagcct tttgcgtcac
     7261 gcgccagcag aaccagcgat gactgccagt gggctaacat ttcttctgtt atgccggtaa
     7321 aaccgaacag cactacgccg tcgatattac gccgttttag gatccccaaa tgttcggcaa
     7381 ctaattgcgg ggaaaactga ctttccatca tgattgggtc gtaaccttgt tcatagaacg
     7441 ctggcagcat ggtttgaacg gcgagatttt ctgacaacga atccaggcgg gtaacaatga
     7501 tggcgaccac tttatcgctt tgcccacgca tagcgcgcgc agagcgggaa ggggaaaatc
     7561 catgctgatt catcactgct tccacgcgct cgcgcgtgcg ctggctcacg ccgctttcgt
     7621 tattcagcac ccgggaaacc gtagatttcc ccacgccgct taagcgcgcg atgtctttga
     7681 tggtcagccg attttgcatc ctgttgtccc gtaacgtgtt gtttaattat ttgaggctaa
     7741 ctttacccgt gcattcagca atgggtaaag tctggtttat cgttggttta gttgccagca
     7801 ggtattatat cgccataaat gctacgaata ttattggatt ctccttatta tttgcggcgc
     7861 ttttttcact taccggaggt tatatggaac ctgatcccac gcctctccct cgacggagat
     7921 taaaactttt tcggtaagcc cgtcttttca cggcgttacc ggatgcgtaa ggccgtgacg
     7981 ttttaacgtc cctgctcagc tttattacct tcaggtaagg cttcgccacg cctgaagaca
     8041 tttctgtact gtttcagaca gtgcggaggg actccttatg tttaaagaaa tttttacccg
     8101 gctcattcgc catttacctt cccgtctggt tcatcgtgat ccattgcctg gcgcgcagca
     8161 gacagtgaat acggtggtcc cgccgtcctt aagtgcgcat tgcctgaaaa tggcggtgat
     8221 gcccgaagaa gaattgtgga aaacgttcga cacccatccg gaagggttaa atcaggcgga
     8281 agtggaatct gcccgcgaac aacatggtga aaataaatta cccgcacaac aaccgtcgcc
     8341 gtggtgggta catttatggg tctgctatcg caaccccttt aatattttac tcaccattct
     8401 cggcgctatt tcttacgcca cggaagattt atttgccgcg ggcgttatcg cgctaatggt
     8461 cgccatttct acgttgctga actttattca ggaagcacgt tccactaaag cggcagatgc
     8521 cctgaaagcg atggtcagca atactgcgac ggtgctgcgc gtaattaacg acaaaggcga
     8581 aaatggctgg ctggagatcc cgatcgacca gctggtgccc ggcgatatta taaaactggc
     8641 ggcgggggat atgatcccgg cagatttacg tatcttgcag gcgcgggatc tgttcgtcgc
     8701 tcaggcgtcg ttaaccggtg agtctctgcc cgtagaaaaa gcagctacca ctcgccagcc
     8761 ggagcacagc aatccgctgg agtgcgacac cctgtgtttt atgggcacca ccgtggtgag
     8821 cggcacggca caagcaatgg tgattgctac aggtgccaat acctggtttg gtcaactggc
     8881 ggggcgtgtt agtgagcagg aaagcgagcc gaatgccttt cagcaaggga tcagccgcgt
     8941 cagtatgctg ctgattcgct ttatgctggt gatggctccg gtggtgctgt taatcaatgg
     9001 ttacaccaaa ggcgactggt gggaagcggc actgtttgcg ctttcggtcg cggtaggctt
     9061 aacgccggaa atgttgccga tgattgttac ctcgacgctg gcgcgcggcg cagtaaagct
     9121 gtcgaaacag aaagtgatcg tcaaacatct ggatgctatt cagaactttg gcgcaatgga
     9181 tattctgtgc actgataaaa ctggcaccct gacacaggac aaaattgtgc tggagaatca
     9241 taccgatatc tccggtaaaa ccagcgaacg cgtgctgcat agcgcgtggt tgaacagtca
     9301 ttatcagacc ggacttaaaa acctgctcga tacagcggtg ctcgaaggta cggatgaaga
     9361 gtcagcgcgc tcgctggcca gtcgttggca aaaaatcgat gagattccgt ttgatttcga
     9421 gcgtcgccgg atgtcggtgg tagtggcaga aaataccgag caccatcagc tggtttgcaa
     9481 aggtgcattg caggaaatcc tcaatgtgtg ttcgcaggtg cgtcacaatg gcgagattgt
     9541 gccgctcgat gacatcatgc tgcgtaagat taagcgggtt actgatacgc tgaatcgtca
     9601 ggggctgcgc gtggttgcgg tggcgacgaa atacctgcca gcgcgtgaag gagattacca
     9661 gcgggcggat gaatccgacc tgatcctcga aggatatatt gctttccttg atccgccaaa
     9721 agagacaact gctccggcac tgaaggcatt aaaagcgagt ggaattaccg taaaaatcct
     9781 tactggcgac agtgagttag ttgcagcgaa agtgtgccat gaagtggggc tggacgcggg
     9841 agaggtggtc attggtagtg atattgaaac gctatctgac gacgaactgg caaatctcgc
     9901 acagcgcacc acgctgtttg ctcgtctgac gccgatgcat aaagaacgca ttgtgacctt
     9961 actgaagcgc gaagggcatg tggttggctt tatgggcgat ggtattaatg atgcgcccgc
    10021 cttacgcgct gcggatatcg gcatttctgt ggacggcgcg gtagatattg cccgtgaagc
    10081 ggctgatatc atcctgctgg aaaaaagcct gatggtgctg gaagaggggg ttattgaggg
    10141 acgtcgcact ttcgccaaca tgctgaaata catcaaaatg acggcgagct ctaacttcgg
    10201 taatgtgttc agcgtgctgg tggcgagtgc tttcttgccc ttcctgccga tgttgccgtt
    10261 acacttgctt attcagaacc tgctgtacga tgtgtcacag gtggcgatcc cgtttgataa
    10321 cgtcgacgac gagcaaattc aaaagccgca gcgttggaat ccggcggatc tggggcgctt
    10381 tatgatcttc ttcggaccga tcagctcgat cttcgatatt ctgacgtttt gcctgatgtg
    10441 gtgggtattc catgccaaca cgccggaaac gcaaacgctg ttccagtcgg gatggtttgt
    10501 ggtgggctta ctgtcgcaaa cgctgattgt gcatatgatc cgtacccgcc gtgtgccgtt
    10561 tattcagagc tgtgcatcgt ggccgttaat gatcatgacc gtgatcgtga tgattgtcgg
    10621 gatcgcattg ccgttttcac cgctggccag ttatctgcag ctgcaggcgc tgccgttaag
    10681 ctatttcccg tggctggttg cgattctggc agggtatatg acgttaaccc agttggtgaa
    10741 agggttctat agccgtcgtt acggctggca gtaaagaata aactgggcac gatagccccg
    10801 attcagattg cagacaaagt gagcgttgtt catgccggat gcggcgtaaa tgccttatcc
    10861 ggcctacaaa ttcaatcaat tgcaggaatc acgtagatac tgataagcga agcgcatcag
    10921 gcaatgatac gtttgtcacc cagcctctgg atcacatcag ttcgggcact tataaacctg
    10981 accgataatc tggctatccg tcgggacaaa actggacaac attccctgcg acgggctgct
    11041 gatgccataa atcacgttac cgcccattgc agccgcctgg ttgcgcagat cgtttgctgc
    11101 gccgcgcata gaaccgccct cttctccgtg ttgcccggaa agccagttgc tttgcttacc
    11161 tgtcgcagta ccaatcagct ggcactctgc gcctggctgc tcgtccacaa tgcgtacact
    11221 ctgaccggca gcactcaatt cgttgctgga gctacaaccc gccatcagta gcgcggcacc
    11281 gacaatccct gctaagtatt ttacgtgcat gttattcccc ataatcaatg agctggacgc
    11341 cactcgcgtc gtccgtgtct gaatcttata ctagaaagat gaccaaaaga aaaaccccca
    11401 gacatttctg cccgggggtt tgtttatttc tgcgaggtgc agggcaatta catcatgccg
    11461 cccatgccac ccatgccgcc cataccgcca gcagcgccta agtcagctgc atcatttttc
    11521 ggcaggtcgg taaccatgca ctcggtggtg atcatcaggc cagccacaga agccgcgtac
    11581 tgcagagcag aacgggttac tttggttggg tccaggatac ccatgtcgat catgttgccg
    11641 tattcttcgg ttgctgcgtt gtaaccgtag ttgccgtcgc cgcctttaac ggtgttagca
    11701 acaacagacg gttcttcgcc gcagttcagg acgatctgac gcagcggagc ttccattgca
    11761 cgcagtgcaa ctttgatacc cacgttctgg tcttcgttct gaccacgcag gtcagccagt
    11821 ttagacgcta cgcggatcag cgcaacacca ccaccagcaa ccacgccttc ttctaccgca
    11881 gcacgggtcg cgtgcagggc gtcttcaacg cgtgcttttt tctctttcat ttcaacttcg
    11941 gtagcagcac ctactttgat aactgcaacg ccgcctgcca gtttcgctac gcgttcctgc
    12001 agtttttcac ggtcgtagtc agaagttgct tcttcaatct gctgacggat ctgagcaaca
    12061 cggccctgga ttgcagcttc ttcacccacg ccatcgatga tagtggtggt gtctttgttg
    12121 atcacaacac gtttagcctg acccaggtct tccagggttg ctttttccag ctccataccg
    12181 atctcttcag agattacggt accgccagtc agggttgcga tatcctgcag catagcttta
    12241 cgacgatcgc cgaagcccgg agctttaacc gcagcaactt tcacgatgcc acgcatggtg
    12301 ttaacaacca gagttgccag cgcttcgcct tcaacatctt cagcgatgat cagcagcggt
    12361 ttgcctgctt tggcaacagc ttccagaacc ggcagcattt cgcggatgtt agagattttc
    12421 ttgtcagcca gcaggatgaa cgggctttcc agttctactg cgccagtttc cggcttgttg
    12481 atgaagtaag gagacaggta gccacggtcg aactgcatac cttcaaccac gtccagttcg
    12541 tcctgcagac cggtaccgtc ttcaacggtg ataacgcctt ctttaccgac tttgtccatc
    12601 gcttcagcga tcagtttacc tacggtttcg tcggagttag cggagatggt accaacctga
    12661 gcaatcgctt tagagtcaga gcacggtacg gacagcgctt tcagttcttc aactgcagcg
    12721 gtaacggctt tgtcgatacc acgtttcagg tccatcgggt tcatgcccgc agcaacagct
    12781 ttcagaccct cagtgatgat agcctgagcc agtacagttg cagtggtggt accgtcgcct
    12841 gcagcgtcgt tcgctttaga ggcaacttct ttcaccatct gcgcacccat attttcgaac
    12901 ttgtcttcca gttcgatttc acgagcaacg gaaacaccat ctttggtgat ggtcggtgca
    12961 ccgaaagatt tatccagaac tacgttacgg cctttcggac cgagggtaac tttcactgca
    13021 tctgccagta cgtttacgcc gcgcagcatt ttcacacgag cgtcgttacc gaattttacg
    13081 tctttagctg ccattatctt tattccttaa attcgtatgt tcagtgtcgt gcgaggatta
    13141 cgcttcaaca attgccagaa tgtcgctttc ggacatgatc aacacttctt cattgtcgat
    13201 cttctcagat ttcacaccgt agccatcgtt gaaaataacg atgtcgccaa ctttcacatc
    13261 cagcggcttc acttcgccat tttcaaggat acggccattg ccgacagcca gcacttcgcc
    13321 gcgggtggat ttagccgctg cagagccggt cagaacgatg ccgccagcag atttagtttc
    13381 aacttcttta cgcttgacga tcacgcgatc atgcaatgga cgaatattca ttgataactc
    13441 tcctttgaga aagtccgtat ctgttatggg tgacgccgga gcttacgtgg tttcccggct
    13501 ggtgaccaga gaaatgggga tgaggattcg ccccttcaag ggggaaaaag aaaaaaaatt
    13561 ctgatcagca caaaatcggg tgaaaaccct gattcacctc acatttcatc gcaatttctt
    13621 catcgccgtc ataagcgaat ctgattgtgc taccatcgaa aatctacgca gttgcccaaa
    13681 atttgggcgc aatcggatat caacccatga gtggactcaa acaagaactg gggctggccc
    13741 aaggcattgg cctgctatcg acgtcattat taggcactgg cgtgtttgcc gttcctgcgt
    13801 tagctgcgct ggtagcgggc aataacagcc tgtgggcgtg gcccgttttg attatcttag
    13861 tgttcccgat tgcgattgtg tttgcgattc tgggtcgcca ctatcccagc gcaggcggtg
    13921 tcgcgcactt cgtcggtatg gcgtttggtt cgcgacttga gcgagtcacc ggctggttgt
    13981 ttttatcggt cattcccgtg ggtttgcctg ccgcgctaca aattgccgcc gggttcggcc
    14041 aggcaatgtt tggctggcat agctggcaac tgttgttggc agaactcggt acgctggcgc
    14101 tggtgtggta tatcggtact cgcggtgcca gttccagtgc taatctacaa accgttattg
    14161 ccggacttat cgtcgcgctg attgtcgcta tctggtgggc gggcgatatc aaacctgcga
    14221 atatcccctt cccggcacct ggtaatatcg aacttaccgg gttatttgct gcgttatcag
    14281 tgatgttctg gtgttttgtc ggtctggaag catttgccca tcttgcctcg gaatttaaaa
    14341 atccagagcg tgattttcct cgcgcattga tgattggtct gctgctggca ggattagtct
    14401 actggggctg tacggtagtc gtcttacact tcgacgccta tggtgaaaaa atggcggcgg
    14461 cagcatcgct tccaaaaatt gtagtgcagt tgttcggtgt aggagcgtta tggattgcct
    14521 gcgtgattgg ctatctggcc tgctttgcca gtctcaacat ttatatacag agcttcgccc
    14581 gcctggtctg gtcgcaggcg caacataatc ctgaccacta cctggcacgc ctctcttctc
    14641 gccatatccc gaataatgcc ctcaatgcgg tgctcggctg ctgtgtggtg agcacgttgg
    14701 tgattcatgc tttagagatc aatctggacg ctcttattat ttatgccaat ggcatcttta
    14761 ttatgattta tctgttatgc atgctggcag gctgtaaatt attgcaagga cgttatcgac
    14821 tactggcggt ggttggcggg ctgttatgcg ttctgttact ggcaatggtc ggctggaaaa
    14881 gtctctatgc gctgatcatg ctggcggggt tatggctgtt gctgccaaaa cgaaaaacgc
    14941 cggaaaatgg cataaccaca taatccggcg tttcgacatt aatcctggcg atcgtcttta
    15001 tgatcaaggc ggtcgcgctc atcatccttt cgctggtact caccatcaaa agtattaccg
    15061 ccaccggtcc cggcgctaaa accgccgcct ggcatacgag aaaagcgcaa atgcggcatc
    15121 aacttcactg tcagatgctt ttgcaccggc ggtaataaaa gcagaagacc gaggaagtcg
    15181 gtgaaaaagc ccggtaataa aagcagcaaa ccggcaatga tcagcgaaac acttttaatc
    15241 atctccgccg ctgggttttc acccgccgcc attttttgct gcatcagcac gaaattctta
    15301 aagccctggt tacgtaccag tgacatcccg ataactgacg tgaatataac cagcacgagg
    15361 gtcagcaata cccccaatac atgggcaacc tgaataaaga ttgaaatctc aatatagaca
    15421 taaaggaaaa tggcaataaa aggtaaccag cgcaaaggtt tctcctgtaa tagcagccgg
    15481 taaaacccgg ctacctgaat gggttgcgaa tcgcgttcag cttatattgt ggtcattagc
    15541 aaaatttcaa gatgtttgcg caactatttt tggtagtaat cccaaagcgg tgatctattt
    15601 cacaaattaa taattaaggg gtaaaaaccg acacttaaag tgatccagat tacggtagaa
    15661 atcctcaagc agcatatgat ctcgggtatt cggtcgatgc aggggataat cgtcggtcga
    15721 aaaacattcg aaaccacata tattctgtgt gtttaaagca aatcattggc agcttgaaaa
    15781 agaaggttca catgtcaaac aacattcgta tcgaagaaga tctgttgggt accagggaag
    15841 ttccagctga tgcctactat ggtgttcaca ctctgagagc gattgaaaac ttctatatca
    15901 gcaacaacaa aatcagtgat attcctgaat ttgttcgcgg tatggtaatg gttaaaaaag
    15961 ccgcagctat ggcaaacaaa gagctgcaaa ccattcctaa aagtgtagcg aatgccatca
    16021 ttgccgcatg tgatgaagtc ctgaacaacg gaaaatgcat ggatcagttc ccggtagacg
    16081 tctaccaggg cggcgcaggt acttccgtaa acatgaacac caacgaagtg ctggccaata
    16141 tcggtctgga actgatgggt caccagaaag gtgaatatca gtacctgaac ccgaacgacc
    16201 atgttaacaa atgtcagtcc actaacgacg cctacccgac cggtttccgt atcgcagttt
    16261 actcttccct gattaagctg gtagatgcga ttaaccaact gcgtgaaggc tttgaacgta
    16321 aagctgtcga attccaggac atcctgaaaa tgggtcgtac ccagctgcag gacgcagtac
    16381 cgatgaccct cggtcaggaa ttccgcgctt tcagcatcct gctgaaagaa gaagtgaaaa
    16441 atatccaacg taccgctgaa ctgctgctgg aagttaacct tggcgcaaca gcaatcggta
    16501 ctggtctgaa cacgccgaaa gagtactctc cgctggcagt gaaaaaactg gctgaagtca
    16561 ctggcttccc atgcgtaccg gctgaagacc tgatcgaagc gacctctgac tgcggcgctt
    16621 atgttatggt tcacggcgcg ctgaaacgcc tggctgtgaa gatgtccaaa atctgtaacg
    16681 acctgcgctt gctctcttct ggtccacgtg ccggcctgaa cgagatcaac ctgccggaac
    16741 tgcaggcggg ctcttccatc atgccagcta aagtaaaccc ggttgttccg gaagtggtta
    16801 accaggtatg cttcaaagtc atcggtaacg acaccactgt taccatggca gcagaagcag
    16861 gtcagctgca gttgaacgtt atggagccgg tcattggcca ggccatgttc gaatccgttc
    16921 acattctgac caacgcttgc tacaacctgc tggaaaaatg cattaacggc atcactgcta
    16981 acaaagaagt gtgcgaaggt tacgtttaca actctatcgg tatcgttact tacctgaacc
    17041 cgttcatcgg tcaccacaac ggtgacatcg tgggtaaaat ctgtgccgaa accggtaaga
    17101 gtgtacgtga agtcgttctg gaacgcggtc tgttgactga agcggaactt gacgatattt
    17161 tctccgtaca gaatctgatg cacccggctt acaaagcaaa acgctatact gatgaaagcg
    17221 aacagtaatc gtacagggta gtacaaataa agaaggcacg tcagatgacg tgcctttttt
    17281 cttgtgagca gtaacttaaa aataacaacc taatatcaac ttgttaaaaa acaaggaagg
    17341 ctaatatgct agttgtagaa ctcatcatag ttttgctggc gatcttcttg ggcgccagat
    17401 tggggggaat aggtattggt tttgcaggcg gattgggggt gctggttctt gccgctattg
    17461 gcgttaaacc cggtaacatc ccgttcgatg tcatttccat tatcatggcg gttatcgccg
    17521 ctatttctgc catgcaggtt gctggcggtc tggactatct ggttcatcag acagaaaagc
    17581 tgctgcgccg taacccgaaa tacatcacga tcctcgcacc gatcgtgacc tatttcctga
    17641 ctatctttgc tggtactggc aacatatctc tggcgacact gccagttatc gctgaagttg
    17701 cgaaggaaca aggcgtcaaa ccttgccgtc cgctgtctac tgcagtggta tccgcgcaga
    17761 ttgcgatcac cgcatcgcca atctcagcgg cagtggttta catgtcttcc gtgatggaag
    17821 gtcatggcat cagctacctc catctgctct ccgtggtcat cccgtccacc ctgctggcgg
    17881 ttctggtgat gtcctttctg gtcactatgc tgttcaactc caaactctct gacgatccga
    17941 tttatcgcaa gcgtctggaa gagggcctgg ttgaactgcg cggtgaaaag cagattgaaa
    18001 tcaaatccgg tgcaaaaacg tccgtctggc tgttcctgct gggcgtagtt ggcgtggtta
    18061 tctatgcaat catcaacagc ccaagcatgg gtctggttga aaaaccgctg atgaacacca
    18121 ccaacgcaat cctgatcatc atgctcagcg ttgcaactct gaccaccgtt atctgtaaag
    18181 tcgataccga caacatcctc aactccagca ccttcaaagc aggtatgagc gcctgtattt
    18241 gtatcctggg tgttgcgtgg ctgggcgata ctttcgtttc caacaacatc gactggatca
    18301 aagataccgc tggtgaagtg attcagggtc atccgtggct gctggccgtc atcttcttct
    18361 ttgcttctgc tctgctgtac tctcaggctg caaccgcaaa agcactgatg ccgatggccc
    18421 tggcactgaa cgtttcaccg ctgaccgctg ttgcttcttt cgctgcggtg tctggtctgt
    18481 tcattctgcc gacctacccg acgctggttg ctgcggtaca gatggatgac acgggtacaa
    18541 cccgtatcgg taaattcgtc ttcaaccatc cgttcttcat cccgggtact ctgggtgttg
    18601 ccctggccgt ttgcttcggc ttcgtgctgg gtagcttcat gctgtaatga ccaatcgcgg
    18661 ggcgttcacg ccccgctttc tttcccgccg actaacatcc ttcccccgtc cgttgtatag
    18721 tgacctctct cttgcggttc catctgttct tgcgaggtgt ttatgcttga tgaagaaagt
    18781 tcgaataccg cgtctgtcgt ggtgctatgt accgcaccgg atgaagcgac agcccaggat
    18841 ttagccgcca aagtgctggc ggaaaaactg gcggcctgcg cgaccttgat ccccggcgct
    18901 acctctctct attactggga aggtaagctg gagcaagaat acgaagtgca gatgatttta
    18961 aaaactaccg tatctcacca gcaggcactg ctggaatgcc tgaagtctca tcatccatat
    19021 caaaccccgg aacttctggt tttacctgtt acacacggag acacagatta cctctcatgg
    19081 ctcaacgcat ctttacgctg atcctgctac tttgcagcac ttccgttttt gccggattat
    19141 tcgacgcgcc gggacgttca cattttgtcc ccgcggatca agcctttgct tttgattttc
    19201 agcaaaacca acatgacctg aatctgacct ggcagatcaa agacggttac tacctctacc
    19261 gtaaacagat ccgcattacg ccggaacacg cgaaaattgc cgacgtgcag ctgccgcaag
    19321 gcgtctggca tgaagatgag ttttacggca aaagcgagat ttaccgcgat cggctgacgc
    19381 ttcccgtaac catcaaccag gcgagtgcgg gagcgacgtt aactgtcacc taccagggct
    19441 gtgctgatgc cggtttctgt tatccgccag aaaccaaaac cgttccgtta agcgaagtgg
    19501 tagccaacaa cgcagcgtca cagcctgtgt ctgttccgca gcaagagcag cacaccgcgc
    19561 aattgccctt ttccgcgctc tgggcgttgt tgatcggtat tggtatcgcc tttacgccat
    19621 gcgtgctgcc aatgtaccca ctgatttctg gcatcgtgct gggtggtaaa cagcggctct
    19681 ccactgccag agcattgttg ctgaccttta tttatgtgca ggggatggca ctgacttaca
    19741 cggcgctggg tctggtagtt gccgccgcag ggttacagtt ccaggcggcg ctacagcacc
    19801 catacgtgct cattggcctc gccatcgtct ttaccttgct ggcgatgtca atgtttggct
    19861 tgtttactct acaactcccc tcttcgctgc aaacacgtct cacgttgatg agcaatcgcc
    19921 aacagggcgg ctcacctggc ggtgtgtttg ttatgggggc gattgccgga ctgatctgtt
    19981 caccatgcac caccgcaccg cttagcgcga ttctgctgta tatcgcccaa agcgggaaca
    20041 tgtggctggg cggcggcacg ctttatctct atgcgttggg catgggcctg ccgctgatgc
    20101 taattaccgt ctttggtaac cgcttgctgc cgaaaagcgg cccgtggatg gaacaagtca
    20161 aaaccgcgtt tggttttgtg atcctcgcac tgccggtctt cctgctggag cgagtgattg
    20221 gtgatgtatg gggattacgc ttgtggtcgg cgctgggtgt cgcattcttt ggctgggcct
    20281 ttatcaccag cctacaggct aaacgcggct ggatgcgcgt ggtgcaaata atcctgctgg
    20341 cagcggcatt ggttagcgtg cgcccacttc aggattgggc atttggtgcg acgcataccg
    20401 cgcaaactca gacgcatctc aactttacac aaatcaaaac ggtagatgag ttaaatcagg
    20461 cgctcgttga agtcaaaggc aaaccggtga tgttagatct ttatgccgac tggtgcgtcg
    20521 cctgtaaaga gtttgagaaa tacaccttca gcgacccgca ggtgcaaaaa gcgttagcag
    20581 acacggtctt acttcaggcc aacgtcacgg ccaacgacgc acaagatgtg gcactgttaa
    20641 agcatcttaa tgtccttggc ctaccgacaa ttctcttttt tgacggacaa ggccaggagc
    20701 atccacaagc acgcgtcacg ggctttatgg atgctgaaac cttcagcgca catttgcgcg
    20761 atcgccaacc gtgaacgaca cttgcagtgg gaaagacgga ggaaaatagc gtgcaacgtg
    20821 aagatgtact gggagaagcc ctgaaattat tagaattaca agggattgcc aacaccacgc
    20881 tggagatggt tgctgaacgt gtggattatc cactggacga gctacgccgc ttctggccag
    20941 acaaagaggc aatcctctac gatgcgctgc gttatcttag ccaacagata gatgtctggc
    21001 gtcgtcagct gatgctggac gaaacgcaaa ccgccgaaca aaagctgctg gcacgttatc
    21061 aggcgttatc ggagtgcgtt aaaaacaacc gctatccggg ctgtctattt atcgctgcct
    21121 gtacgtttta tcccgatcct ggccacccta ttcatcaact ggccgatcag caaaaaagcg
    21181 cggcctacga tttcacccac gaactgttaa ccacgctgga agttgacgat ccggcgatgg
    21241 tagcaaagca gatggaactg gtgctggaag gctgtttaag ccgaatgctg gtgaatcgta
    21301 gccaggcgga tgtcgacact gcacatcggc tggcggaaga tatcctgcgc ttcgcccgct
    21361 gccgccaggg tggtgcattg acctgacaga aacacagaaa agaagcgatt tgccgcaatc
    21421 ttaagcagtt gaatcgcttt tactgaaatt aggttgacga gatgtgcaga ttacggttta
    21481 atgcgccccg ttgcccggat agctcagtcg gtagagcagg ggattgaaaa tccccgtgtc
    21541 cttggttcga ttccgagtcc gggcaccaaa ttcatatcaa cggacctcca cggaggtccg
    21601 tttttcgttt cagaacacca tgatttaagt gttcttcc
//
LOCUS       DAZGJW010000073        21513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZGJW010000073 DAZGJW010000000
VERSION     DAZGJW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.073"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            212..1255
                     /gene="nadA"
                     /locus_tag="ACHKIK_002825"
     CDS             212..1255
                     /gene="nadA"
                     /locus_tag="ACHKIK_002825"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO9083966.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            1293..2012
                     /gene="pnuC"
                     /locus_tag="ACHKIK_002826"
     CDS             1293..2012
                     /gene="pnuC"
                     /locus_tag="ACHKIK_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO9083967.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRLAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(2009..2950)
                     /gene="zitB"
                     /locus_tag="ACHKIK_002827"
     CDS             complement(2009..2950)
                     /gene="zitB"
                     /locus_tag="ACHKIK_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO9083968.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(3064..3444)
                     /locus_tag="ACHKIK_002828"
     CDS             complement(3064..3444)
                     /locus_tag="ACHKIK_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO9083969.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            3760..4812
                     /gene="aroG"
                     /locus_tag="ACHKIK_002829"
     CDS             3760..4812
                     /gene="aroG"
                     /locus_tag="ACHKIK_002829"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO9083970.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGETAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(4971..5723)
                     /gene="gpmA"
                     /locus_tag="ACHKIK_002830"
     CDS             complement(4971..5723)
                     /gene="gpmA"
                     /locus_tag="ACHKIK_002830"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO9083971.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(5926..6966)
                     /gene="galM"
                     /locus_tag="ACHKIK_002831"
     CDS             complement(5926..6966)
                     /gene="galM"
                     /locus_tag="ACHKIK_002831"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO9083972.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(6960..8108)
                     /gene="galK"
                     /locus_tag="ACHKIK_002832"
     CDS             complement(6960..8108)
                     /gene="galK"
                     /locus_tag="ACHKIK_002832"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO9083973.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYYLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(8112..9158)
                     /gene="galT"
                     /locus_tag="ACHKIK_002833"
     CDS             complement(8112..9158)
                     /gene="galT"
                     /locus_tag="ACHKIK_002833"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO9083974.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTRTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(9168..10184)
                     /gene="galE"
                     /locus_tag="ACHKIK_002834"
     CDS             complement(9168..10184)
                     /gene="galE"
                     /locus_tag="ACHKIK_002834"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO9083975.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(10445..11917)
                     /gene="modF"
                     /locus_tag="ACHKIK_002835"
     CDS             complement(10445..11917)
                     /gene="modF"
                     /locus_tag="ACHKIK_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO9083976.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(11985..12773)
                     /gene="modE"
                     /locus_tag="ACHKIK_002836"
     CDS             complement(11985..12773)
                     /gene="modE"
                     /locus_tag="ACHKIK_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO9083977.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            12902..13051
                     /gene="acrZ"
                     /locus_tag="ACHKIK_002837"
     CDS             12902..13051
                     /gene="acrZ"
                     /locus_tag="ACHKIK_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO9083978.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            13217..13990
                     /gene="modA"
                     /locus_tag="ACHKIK_002838"
     CDS             13217..13990
                     /gene="modA"
                     /locus_tag="ACHKIK_002838"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9083979.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEEHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            13990..14679
                     /gene="modB"
                     /locus_tag="ACHKIK_002839"
     CDS             13990..14679
                     /gene="modB"
                     /locus_tag="ACHKIK_002839"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO9083980.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            14682..15740
                     /gene="modC"
                     /locus_tag="ACHKIK_002840"
     CDS             14682..15740
                     /gene="modC"
                     /locus_tag="ACHKIK_002840"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO9083981.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(15741..16559)
                     /gene="ybhA"
                     /locus_tag="ACHKIK_002841"
     CDS             complement(15741..16559)
                     /gene="ybhA"
                     /locus_tag="ACHKIK_002841"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO9083982.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            16714..17709
                     /gene="pgl"
                     /locus_tag="ACHKIK_002842"
     CDS             16714..17709
                     /gene="pgl"
                     /locus_tag="ACHKIK_002842"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO9083983.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYSIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(17750..18703)
                     /locus_tag="ACHKIK_002843"
     CDS             complement(17750..18703)
                     /locus_tag="ACHKIK_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9083984.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            18887..19939
                     /locus_tag="ACHKIK_002844"
     CDS             18887..19939
                     /locus_tag="ACHKIK_002844"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO9083985.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            20015..21448
                     /locus_tag="ACHKIK_002845"
     CDS             20015..21448
                     /locus_tag="ACHKIK_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO9083986.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
BASE COUNT         5112 a         5307 c         5601 g         5493 t
ORIGIN      
        1 caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta ctgtgcaagc
       61 aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca ttcgactatc
      121 ttgtttagca tataaaacaa attacaccga taacagcgaa tattacgcta atgtcggttt
      181 taacgttaag cctgtaaaac gagatggtaa gatgagcgta atgtttgatc cagacacggc
      241 gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag cgtattaccg
      301 cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc actactatac
      361 cgatcccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg attctctgga
      421 aatggcgcgc ttcggtgcaa agcatcccgc ttctactttg ttagtcgctg gggtgagatt
      481 tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc cgacacttca
      541 ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat tttgcgatgc
      601 ccatcccgat cgtactgtcg tcgtctacgc caacacttct gctgcggtaa aagcgcgcgc
      661 agattgggtg gtaacttcaa gcattgccgt cgaacttatt gatcatcttg atagtttggg
      721 tgaaaaaatc atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa aacagacggg
      781 tggagacatt ctatgctggc agggtgcctg tattgtgcat gatgaattta agactcaggc
      841 gttaacccgc ttgcaagaag aatacccgga tgctgccata ctggtgcatc cagaatcacc
      901 acaagctatt gtcgatatgg cggatgcggt cggttccacc agtcaactga tcgctgctgc
      961 gaaaacattg ccacatcagc ggcttatcgt ggcaaccgat cggggcattt tctacaaaat
     1021 gcagcaggca gtgccagata aagagttact ggaagcacca accgcaggtg agggtgcaac
     1081 ctgccgcagc tgcgcgcatt gtccgtggat ggccatgaat ggccttcagt ccatcgcaga
     1141 ggcattagaa caggaaggaa gcaatcacga ggttcatgtt gatgaaaggc tgcgagagag
     1201 ggcgctggtg ccgctcaatc gtatgctgga ttttgcggct acactacgtg gataacgaat
     1261 aataaggcgt aacgttacgc tttgggggaa agatggattt ttttagtgtg cagaatatcc
     1321 tggtacatat accaataggg gcaggcggtt atgatctctc atggatcgaa gcggtaggca
     1381 cgatcgccgg gttgctgtgt attggccttg ccagtctgga gaagatcagc aactacttct
     1441 ttggcctgat caacgtcacc ttgtttggca ttattttctt tcagattcag ctgtatgcca
     1501 gcctgctatt acaggtgttt ttctttgccg cgaatattta cggttggtat gcgtggtcgc
     1561 gacaaaccag tcagaacgag gcggagttga aaattcgctg gttgccattg ccgaaggcac
     1621 tcagctggtt ggcggtttgc gttgtttcga ttggtctgat gacggtattt atcaatccgg
     1681 tgtttgcatt tttgacccgc ctggcagtca tgatcatgca agcattagga ttacaggttg
     1741 tgatgcctga actgcaaccg gacgctttcc cgttctggga ttcatgcatg atggtgttat
     1801 ctatcgtggc aatgattctg atgacgcgta agtatgtgga aaactggctg ttgtgggtga
     1861 ttattaacgt gattagcgtc gttatttttg cacttcaggg cgtttacgcc atgtctctgg
     1921 agtacatcat cctgaccttt attgcgctca acggcagccg gatgtggatc aacagcgcac
     1981 gtgaaagagg ctcacgcgcg ctgtcccatt aatggtgatg atgtgaatgg ccagacaccc
     2041 cttcattcag gtggcagtct ggcccatgac aaggttgata ttccatctga atggtggcgt
     2101 gctcaatctg atagtgatcc atcaggtagt gttggatctg atccaacaag gcatcgtgat
     2161 cgtgtggcgg gatcacctgc acatgcaacg tcatcaccgg cttctcgcct accatccata
     2221 catgtacatg gtgcacattg cggacttccg ggatttcccg gcacatacga cgcttcagtt
     2281 cagcgatatc cagcgatacc ggtgcacctt caagtaattc attcacgcta tctttcaaca
     2341 gtcgccacgc gctacgcaga accagaagcg acaccagtat cgagagaatg gggtcagcag
     2401 gtgtccagcc agtccagata ataatcaacg cggcaataat agccccaacc gaacccagca
     2461 ggtccccgag cacatgcagt gccgctgctc gtacgttgag gtttttctct tcgctgccgt
     2521 gatgaagtaa ccagaaagaa agtatatttg ccagcaaccc agccacggca attgccatca
     2581 tcatgccgcc ctcgaccgga cgcggcgtgc ggaagcgttc tatcgcctcc cagacaatca
     2641 aaatggtaat taccacgagg gctatggcat tcacaaacgc cgcgagcgtg gtcagtctta
     2701 gccaaccgaa agtgtggcga atggtgggag gacgacgtga aaattgcacg gcgagtaggg
     2761 cgaaaagcag ggcggcagta tcggtcaaca tatgacccgc atcggccagc aatgccagag
     2821 aaccagaaag aaaaccacca acgacttcta ccagcataaa cccggcagtc acgccgaaag
     2881 catacaacaa gcgacgagca ttattatctt ctggcaggtg tgaggacgtg tgtgagtgtg
     2941 agtgcgccat gacgagtcat cccttaatta ttctcactta actttatgac atcatactgc
     3001 ttttagaagt gaaaaattaa aagggagaga ctccgctctc ccattattgg ctattttgca
     3061 gggttactgc gtggtaccgt cggttttggt atcgacatca ttattgatgc catcaccggt
     3121 ttgtaccttt ttattgatat ccggacagcg accatctttg cacatggtgt tcttgtgctc
     3181 ttcatctttg gtcattccgt cattgttcat tgaagaacca tccgaatgca gcattgtgcc
     3241 accagaaccg gtatttaccc cgttattgtc gacgttattt ggcgcgacat tttcacgggc
     3301 gtcaggggct acctggcccg catcagctgc ggcgtttgcc tggccgttat tagtttgcgc
     3361 tccgctatcg gcagccagtg cggcaccgct ggcaaggctt agagtggcag tcagaaataa
     3421 tgtggccagt tttgtcattt tcataggatg ctcctgttat ggtcgttatg tcggataacc
     3481 tcttccaaca gtgcatttgc aggtgaatat aaggcattgg tttaagattt cagccaggtt
     3541 atgaaacgca gcagagaatc ttgaaataat taacaaacaa aggagttaca gttagaaatt
     3601 gtaggagaga tctcgttttt cgcgacaatc tggcgttttt cttgctaatt ccaggattaa
     3661 tccgttcata gtgtaaaacc ccgtttacac attctgacgg aagatataga ttggaagtat
     3721 tgcattcact aagataagta tggcaacact ggaacagaca tgaattatca gaacgacgat
     3781 ttacgcatca aagaaatcaa agagttactt cctcctgtcg cattgctgga aaaattcccc
     3841 gctactgaaa atgccgcgaa tacggttgcc catgcccgaa aagcgatcca taagatcctg
     3901 aaaggtaatg atgatcgcct gttggtggtg attggcccat gctcaattca tgatcctgtc
     3961 gcagcaaaag agtatgccac tcgcttgctg gcgctgcgtg aagagctgaa agatgagctg
     4021 gaaatcgtaa tgcgcgtcta ttttgaaaag ccgcgtacca cggtgggctg gaaagggctg
     4081 attaacgatc cgcatatgga taacagcttc cagatcaacg acggtctgcg tatagcccgt
     4141 aaattgctgc ttgatattaa cgacagcggt ctgccagcgg cgggtgagtt tctcgatatg
     4201 atcaccccac aatatctcgc tgacctgatg agctggggcg caattggtgc acgtaccacc
     4261 gaatcgcagg tgcaccgcga actggcgtct ggtctttctt gtccggtagg ttttaaaaat
     4321 ggcacagacg gtacgattaa agtggctatc gatgccatta atgccgccgg tgcgccgcac
     4381 tgcttcctgt ccgtaactaa atgggggcat tcggcgattg tgaataccag cggtaacggt
     4441 gattgccata tcattctgcg cggcggtaaa gagcctaact acagcgcgaa gcacgttgct
     4501 gaagtgaaag aagggctgaa caaagcaggc ctgccagcac aggtgatgat cgatttcagc
     4561 catgctaact cgtccaaaca attcaaaaag cagatggatg tgtgtgctga cgtttgccag
     4621 cagattgccg gtggcgaaac agccattatt ggcgtgatgg tggaaagcca tctggtggaa
     4681 ggcaatcaga gcctcgagag cggggagccg ctggcctacg gtaagagcat caccgatgcc
     4741 tgcatcggct gggaagatac cgatgctctg ttacgtcaac tggcgaatgc agtaaaagcg
     4801 cgtcgcgggt aaggtttaat tgtcggatgc gccgtcagag tggcgtatcc gatgaatcac
     4861 cacaggcctg ataagacgcg cagcgtcgca tcaggcaatg tgctccattg ttagcaacaa
     4921 aaaagccggc tcacttgcag tcggctttct catttttaaa cgaatgacgt ttacttcgct
     4981 ttaccctggt tagcaaccgc cgctgctttc gctgcgatct cgtcagcatt acccagataa
     5041 tagcgtttca gcggtttgaa attctcgtcg aactcataca ccagcggcac gccggtcggg
     5101 atattcagct caagaatctc ttcttcactc atgttatcaa gatatttcac cagcgcacgt
     5161 aaagagttac cgtgtgcagc gatgatcacg cgctcaccgc tcttcatacg cggtagaata
     5221 gtttcattcc agtaagggat cacgcggtca atggtcagcg ccaggctttc cgtcagcggc
     5281 agttctttct cgctcagctt cgcgtaacgt ggatcgtgac ccggataacg ctcatcatct
     5341 ttagtcagtt ccggcggagt gactgcaaaa ccacgacgcc actgtttcac ctgctcgtcg
     5401 ccatactttt cagcggtttc tgctttgttc agaccctgca acgcaccgta gtgacgttcg
     5461 ttcagtttcc aggatttctc aacgggcagc catgcctgat ccagttcgtc cagcacattc
     5521 cacagggtat ggatagcgcg tttcagcaca gaagtgtaag caaagtcaaa gctgtaacct
     5581 tcctctttca gcagcttacc tgctgctttt gcttcgctta cgcctttctc agacagatcc
     5641 acgtcgtacc aaccggtgaa acggttttct ttgttccact gactttcgcc atgacgaacc
     5701 agaaccagct tagttacagc catatactta ctcctcaaat catcttttaa tgataataat
     5761 tctcattata ttgccgtgac gaagcaacag caatgcttac gcataaccat agcgaaaata
     5821 gtggcgcagt gtaaggttgt tgtgaatatt gagttgcaaa tatgtcggtg tttgctggtg
     5881 atttgaacaa tatgaagata aagccctcat gacgagggct taacattact cagcaataaa
     5941 ctgatattcc gtcaggctgg aatactcttc gccaggacgc aggaagcagt ccggttgcgg
     6001 ccattcaggg tggttcgggc tgtccggcag aaactcgctt tccaacgcca gcccttgcca
     6061 gtcggcgtaa ggttcggttc cccgcgacgg tgtgccgccg aggaagttgc cggagtagaa
     6121 ttgaagagcc ggagcggtgg tgtagacctt cagctgcaat ttttcatctg ctgaccagac
     6181 atgcgccgcc actttcttgc catcgccttt ggcctgtaac aagaatgcgt gatcgtaacc
     6241 tttcactttg cgctgatcgt cgtcggcaag aaactcactg gcgatgattt tggcgctgcg
     6301 gaaatcaaaa gacgttccgg cgacagattt caagccgtcg tgcggaatgc cgccttcatc
     6361 aaccggaaga tattcgtccg ccagaatctg caacttgtga ttgcgcacgt cagactgctc
     6421 gccgtcaaga ttgaaataga cgtgattagt catattcacc gggcaaggtt tatcaactgt
     6481 ggcgcgataa gtaatggaga tacggttatc gtcggtcaga cgatattgca ccgttgcgcc
     6541 gagattaccc gggaagccct gatcgccatc atctgaactc agggcaaaca gcacctgacg
     6601 atcgttctgg ttcacaatct gccagcgacg tttgtcgaac ccttccgggc cgccgtgcag
     6661 ctggttaacg ccctgacttg gcgaaagggt cacggtttca ccgtcaaagg tataacggct
     6721 attggcgatg cggttggcat aacgaccaat agaggccccc agaaacgcgg cctgatcctg
     6781 atagcattcc gggctggcac agccgagcag cgcctcgcgg acgctgccat cggaaagcgg
     6841 aatacgggct gaaagtaaag tcgcacccca gtccatcagc gtgactacca tccctgcgtt
     6901 gttacgcaaa gttaacagtc ggtacggctg accatcgggt gccagtgcgg gagtttcgtt
     6961 cagcactgtc ctgctccttg tgatggttta caaacgtaaa aagtctcttt aatacctgtt
     7021 tttgcttcat attgttcagc gacagcttgc tgtacggcag gcaccagctc ttccgggatc
     7081 aacgcgacga tacagccgcc aaatccgccg ccggtcatgc gtacgccacc tttgtcgcca
     7141 atcacagctt tgacgatttc taccagagtg tcaatttgcg gcacggtgat ttcgaaatca
     7201 tcgcgcatag aggcatgaga ctccgccatc aactcgccca tacgtttcag gtcgccctgc
     7261 tccagcgcgc tggcagcttc aacggtgcgg gcgttttcag tcaggatatg ccgcacgcgt
     7321 ttcgccacga ttgggtccag ctcatgtgca acagcgttga actcttcaat ggtgacatcg
     7381 cgcagggctg gctgctggaa gaaacgcgca ccggtttcgc actgttcacg acgggtgttg
     7441 tattcgctgc caaccagggt acgtttgaag ttactgttga tgatgacgac agccacacct
     7501 ttcggcatgg aaactgcttt ggtccccagt gagcgacagt caatcagcaa ggcatgatct
     7561 ttcttgccga gtgcggaaat tagctgatcc atgatcccgc agttacagcc aacaaactgg
     7621 ttttctgctt cctgaccgtt aagcgcgatt tgtgcgccgt ccagcggcag ataataaagc
     7681 tgctgcaata cggttccgac cgcgacttcc agtgaagcgg aagaacttaa cccggcaccc
     7741 tgcggcacat tgccgctgat caccatgtcc acaccgccga agctgttgtt acgcagttgc
     7801 agatgtttca ccacgccacg aacgtagttc gcccattgat agttttcatg cgcgacaatg
     7861 ggcgcattga gggaaaactc gtcgagctga ttttcataat cggctgccat tacgcgaact
     7921 ttacggtcat cgcgtggcgc acagctgatc acggtttgat aatcaatcgc gcagggcaga
     7981 acgaaaccgt cgttgtagtc ggtgtgttca ccaatcaaat tcacgcggcc aggcgcctga
     8041 atggtgtgag tggcagggta gccaaatgcg ttggcaaaca gagattgtgt tttttctttc
     8101 agactcattt cttacactcc ggattcgcga aaatggatat cgctgactgc gcgcaaacgc
     8161 tctgctgcct gttctgcggt caggtctcgc tgggtctctg ccagcatttc ataaccaacc
     8221 ataaatttac gtacggtggc ggagcgcagc agaggcggat aaaagtgcgc gtgcagctgc
     8281 cagtgttgat tctcttcgcc attaaatggt gcgccgtgcc agcccataga gtaggggaag
     8341 gagcactgga agaggttgtc ataacgactg gtcagctttt tcaacgccag cgccaaatcg
     8401 ctgcgctggg cgtcggtcaa atcggtgatc cgcaaaacgt gggctttggg cagcagtagc
     8461 gtttcgaacg gccaggcagc ccagtaaggc acaacggcta accagtgttc ggtttcgaca
     8521 acggtacggc taccgtctgc cagctcgcgc tgaacataat ccaccagcat tggtgatttc
     8581 tgttcggcaa aatattcttt ttgcaggcgg tcttcgcgct cagcttcgtt aggcaggaag
     8641 ctatttgccc aaatctgtcc gtgcggatgc gggttagagc agcccatcgc cgcacctttg
     8701 ttttcaaaga cctgcaccca cgggtacgtt tttcccagct ctgcggtttg ctcctgccag
     8761 gttttgacga tttccgtcaa tgccgcaacg ctcagttctg gcaacgtttt actgtgatcc
     8821 ggtgaaaagc agatcacccg gctggtaccg cgcgcgctct ggcaacgcat caaaggatcg
     8881 ttgctttctg gcgcatctgg cgtgtcagac atcaaggccg caaagtcatt agtgaaaacg
     8941 taagtcctgg tgtaatcggg gtttttatcg cctgtcaccc gcacattacc tgcgcagagg
     9001 aagcaatctg gatcgtgcgc aggtaacacc tgtttggctg gcgtttcctg cgccccctgc
     9061 caggggcgct tagcgcggtg cggtgaaacc agaatccatt gcccggtgag cgggttgtag
     9121 cggcgatgtg gatgatcaac gggattaaat tgcgtcatgg tcgttcctta atcgggatat
     9181 ccctgtggat ggcgtgactg ccagtgccag gtgtcctgcg ccatttcatc gagtgtgcgc
     9241 gttacgcgcc agttcagttc acggtcggct ttgctggcgt ccgcccagta ggccggaagg
     9301 tcgccctcgc gacgcggtgc aaaatgataa ttaaccggtt tgccgcaggc tttgctgaag
     9361 gcattaacca cgtccagcac gctgttgcct acgccagcgc cgaggttgta gatgtgtacg
     9421 cctggcttgt tcgccagttt ttccatcgcc acgacgtgac cgtccgccag atccattacg
     9481 tggatgtaat cgcgtacgcc agtaccatct tcggtcggat aatcgttacc aaaaatcgcc
     9541 agcgagtcgc gacggcctac agcaacctgg gcgatgtatg gcatcaggtt attcggaatg
     9601 ccttgcggat cttcgcccat atcgcccgac ggatgcgcgc caaccgggtt gaagtagcgc
     9661 agcagggcaa tgctccagtc cggctgggct ttttgcagat cggtgaggat ctgttccacc
     9721 atcagcttgc ttttgccgta agggctttgt ggtgtgccgg tcgggaagct ttcaacgtat
     9781 ggaattttgg gctgatcgcc ataaacggtg gcggaggagc taaaaataaa gtttttgacg
     9841 ttagcggcgc gcatggcgct aatcaggcgc agagtgccgt tgacattgtt gtcgtaatat
     9901 tccagcggtt tttgtaccga ttcgccgacg gctttcagcc cggcgaagtg gatcacggtg
     9961 tcgatagcgt gatcgtgcag tatctcggtc atcaacgctt cgttacgaat atcgccttca
    10021 acaaacgttg gatgtttgcc gcctaaacgc tcgataacag gcagtacgct gcgcttactg
    10081 ttacagaggt tatcaagaat gatgacatca tgaccgtttt gcagtaattg cacacaggta
    10141 tgacttccaa tgtaaccgct accaccggta accagaactc tcataattcg ctccattagg
    10201 cttatggtat gaaataacca tagcataaca aagatgcgaa aagtgtgaca tggaataaat
    10261 tagtggaatc gtttacacaa gaatttagcc gttttttatg cgcgattaag tgattataaa
    10321 acagagggtt tatgaatgat tgcgcttttt atctgaaaaa agaagcggtt tcatgcctgc
    10381 atgcgtcgaa ccgttggccg gagagggcgc ttaggccgcc tccggcaagg tcagcactac
    10441 cgactcaata tatttttgtc agcacatagc gatagagtcc accgtccggc acgaactcaa
    10501 ggcgatgggt aatacaggca ggcgcatctt cagcgtggtg cgaaacaaac aacaattgcg
    10561 tttcaccttc gctaatcagc acatcaacaa aacggcggat aagctggcga ttgagcggat
    10621 caagcccctg tagtggttca tcgagaataa gcaacgtcgg atgtttcacc agtgcgcgga
    10681 cgatcagcgc cagacgctgc tgtccccagg aaagactatg gaacggagcg tcagccgtac
    10741 gtttatcaat gccgagaata tccagccact gctgcaccag tttttgctgg cgatccgaaa
    10801 cggcctgata aatgccaatc gaatcaaaat agccagaaag aatcacatta cgcacggtag
    10861 tgctgacccg gtaatccaga tgcaaactac tgctgacgta accgatatgc tttttgatat
    10921 cccagatggt ttcgccgctg ccgcgacgtc gtccgaaaag cgtcaaatcg ttgctgtaac
    10981 cttgcggatg atcgccagta accaggctta ataacgtcga ttttcctgca ccatttggcc
    11041 cgacaatttg ccagtgttca cctggattca cctgccagct aaggttatta agaatggggc
    11101 gatcgttata agaaaccacg ccattgttca gcacaatgcg cggttcgttg gcgggtaagg
    11161 cgtgacgtgc tgaaggttca tccggctccg gcagttgcac accttcaagc tgttcactat
    11221 gcgccagttg cgcgacgagt gcttgttgga gcagttcctc tttagcgcca gtttccgcta
    11281 acgtgcaatc cgccagcacg ccagcaaact ggacaaactc cgggatctca tcgaagcgat
    11341 tgagcaccag taccagagta ataccggact gatgtaacga ggcgagtcgc tcagccagct
    11401 gctgacgtga ggcaacatcc aggccatcga acggctcatc aagaatcaac aagtcaggct
    11461 ccgacatcag cgcctgacac agcagggttt ttcgcgtctc gccagtggaa aggtatttaa
    11521 agcgtcggtc gaggagggcg gtaataccga actgctgcgc cagttgcatg caacgcggtg
    11581 catcctttac ttcatcctga atgatctcag ccgtagtgcg tccggtgtca tcttcgccag
    11641 ggccgagcat atcggtgtta ttccgctgcc attcgtcgct gacgagtttt tgcaattgct
    11701 cgaaggagag acgagtgatg tgggaaaact ggctttgccg ttcacctttc aaaagcggaa
    11761 gttcccccgc cagcgcgcgg gccagggccg atttcccgct tccattcgaa ccgacaaacg
    11821 cccaactatc acccgcgttt aacgttagct gaggcaattg cagcgttttt gtgtcgctaa
    11881 gacgaaacgt gccttgcaaa atttgcaacg atgacatttt atatcccatt ttgtgcagcg
    11941 attactgaca gggatacgtg tttcataaca aattgtcaac acgcttagca cagcgtggcg
    12001 ataatcacgc tgtcggcatt aaagtaggcc gtgacattct gcccttgctg aagagaagtc
    12061 gcttcattta ccggcactgt ggcgcacagt gtttgcccgt cgggtagcgc cattaatact
    12121 tcgcactgct ctgcgccgcg ctcaatatga ctaataatac ccggtaattg gttgtcggcg
    12181 ttttgtgcga ccgcctcgtc ctgagtaata cctacccacg gcgcttttag caatatcaac
    12241 acttctttgc cttcatccag ccccagacgc gcgccgcttt gtgcggtaat tgcgactttc
    12301 aggcgtgttt ttccgtcagc cagtaagaca tcaacatgct gttgaacgtc atcatgatcg
    12361 cgggcggtga tggtgccgaa ccactggtta cgggcgctgg tttgcagtga aaaacgtgag
    12421 atcgcggcca gcaggctgtt cagcggcagg gcgtcatcgt cacttaacac atcaaaggct
    12481 ttttgctgga tttgcgccag taagtcatag agctgaatca gtcgctgacc atagcgggtc
    12541 agtactgcgc cgccgccacc tttaccgcct gttgcgcgct cgaccagaat atgctcactt
    12601 aactgattca tctcgttaat ggcatcccag gcgcttttat agctgatacc ggcatctttc
    12661 gctccctggc taatggaacc ggaaagcgca atgtgtttta gtagcgaaat ccggcgcggg
    12721 tcggcgaata atttttgttg gagcttaagg gtgagaagga tttcggcctg cataacaatg
    12781 tcctggcaaa agtcttattg tgacggaaaa cgaacgccac gcaaagctga ccgcacaaaa
    12841 ggggagtgct tttctgtgct tagcggttag aatagtctca tgactatatc tggagttgac
    12901 catgttagag ttattaaaaa gtctggtatt cgccgtaatc atggtacctg tcgtgatggc
    12961 catcatcctg ggtctgattt acggtcttgg tgaagtattc aacatctttt ctggtgttgg
    13021 taaaaaagac cagcccggac aaaatcattg attccctgaa tgcccgctta gtcgggcatt
    13081 tctttttctc aacttcctgc ttttccagcc gatatttttt cttatctacc tcacaaaggt
    13141 tagcaataac tgctgggaaa attctgagct agccgttata ttgtcgccta cataacgtta
    13201 cattaagggg ttaccaatgg ctcgtaaatg gttgaacttg tttgccgggg cggcactctc
    13261 tttcgctgtt gctggcaata cactggcaga tgaagggaaa atcacggtgt tcgccgccgc
    13321 atcactgact aacgcaatgc aggacattgc tacgcagtat aaaaaagaaa aaggcgtgga
    13381 tgtggtttct tctttcgctt cgtcatctac tctggcccgt cagattgaag cgggagcgcc
    13441 tgcggatctg tttatctctg ccgatcagaa atggatggat tatgcggttg ataaaaaagc
    13501 gatcgatacg gcttcgcgcc agacactgct cggcaatagc ctggtcgttg tagcaccgaa
    13561 agccagcgag cagaaagatt tcaccatcga cagcaaaacc aactggactt cgctgctgaa
    13621 tggcggtcgc ctcgcggttg gcgatccgga acatgtccct gctggcattt atgcgaaaga
    13681 agcactgcaa aaactgggcg catgggatac gctctccccg aaactggctc cggcggaaga
    13741 tgttcgtggc gcgctggcgt tggtcgaacg caatgaagcg ccgttgggca ttgtttacgg
    13801 ttctgacgca gttgccagca aaggggtaaa agtggttgcc accttcccgg aagattcaca
    13861 taaaaaagtg gaatatccgg ttgctgttgt ggaagagcat aacaatgcga cagtgaaagc
    13921 attttatgat tatttgaaag ggccgcaggc tgcggaaatc tttaaacgtt acggatttac
    13981 aaccaagtaa tgatactgac cgatccagaa tggcaggcag ttttattaag cctgaaagtt
    14041 tcttccctgg ctgtgctgtt tagcctgccg tttgggatct tttttgcctg gttactggtg
    14101 cgttgcacgt ttccgggcaa agctctgctc gacagcgtac tgcatctacc gctggtgtta
    14161 ccgcccgtgg tcgtcggtta cttattatta gtttcgatgg gacggcgcgg atttatcggt
    14221 gaacgtctgt atgactggtt tggtattacc ttcgccttta gctggcgcgg cgcggttctc
    14281 gctgccgccg tcatgtcgtt tccgctgatg gtgcgggcaa ttcgtctggc gctggaaggg
    14341 gttgatgtca aactcgaaca ggccgcaaga acactggggg ccgggcgctg gcgcgttttc
    14401 tttactatca cgttaccgct gaccttaccg ggaattattg ttggtacggt actggctttt
    14461 gctcgttctc tcggtgagtt tggtgcaacc atcacctttg tgtcgaacat tcctggtgaa
    14521 acgcgaacca ttccttctgc catgtatacc ctgatccaga cccccggcgg ggaaagtggc
    14581 gcggcgagac tgtgcattat ttctattgcg ctggcgatga tctccctgtt gatttcagaa
    14641 tggctggcca gaatcagccg tgaacgggcg gggcgctaat catgctggaa ctgaattttt
    14701 cccagacctt gggcaaccat tgcctgacca ttaatgaaac gctgcccgcc aatggcatca
    14761 ctgctatctt tggcgtctcc ggtgccggaa aaacttcgct gattaacgcc atcagtggac
    14821 tgacgcgccc gcaaaaaggg aggattgtcc tcaatggtcg ggtactaaat gatgccgaaa
    14881 aaggtatctg cctgacgcca gaaaagcgtc gcgttggcta tgtttttcag gatgcgcggc
    14941 tgtttccgca ttacaaagtg cgtggcaatc tgcgctacgg catggcgaaa agcatggtcg
    15001 atcagttcga taagctggtg gcgcttttag gcattgaacc gttgcttgac cgtttaccag
    15061 gcagcctgtc cggaggcgaa aaacagcgcg tagcgattgg gcgggctttg ctgacggcac
    15121 cagaattgct gttgctggat gaaccgctgg cgtcactgga tattccgcgt aaacgcgaac
    15181 tgttgcctta tctgcaacgt ctgacgcggg aaatcaacat cccgatgttg tatgtcagcc
    15241 attcgctgga tgagatcctc catctggcag acagagtgat ggtactggaa aacggtcagg
    15301 tgaaagcctt tggcgcactg gaagaagtct ggggtagtag cgtgatgaat ccgtggctgc
    15361 cgaaagagca acaaagtagc attctgaaag tgacggtact tgagcatcat ccgcattacg
    15421 cgatgactgc gctggcgctg ggcgatcagc atttgtgggt caataagctg gacgaaccgc
    15481 tgcaagctgc gctacgtatc cgcattcagg cttccgatgt ttctctggtt ttacaaccgc
    15541 cgcagcaaac cagcattcgt aacgtattgc gggcaaaagt tgttaatagt tatgacgaca
    15601 acggccaggt ggaagtggaa ctggaagtcg gaggtaaaac gctgtgggcg cgtatcagcc
    15661 cgtgggccag ggatgaactg gtgatcaaac ctggcctgtg gctgtacgcg caaattaaaa
    15721 gtgtgtcgat aaccgcctga ttaaatcagg tggctataaa tgaactgggc aatgctgtcg
    15781 gtggtgttat caccaatcac aatgttggcg cgcgctttta ccgcgtcatc ggcgttgccc
    15841 atcgccacgc ctgtaccagc ggcctccagc atactgatat cattaaagtt atcgccgaac
    15901 gccacgacgt tttccatcga ccagccttgc gcctcaaccc atttcgtcaa acgtttacct
    15961 ttgctgttgc cgccgcgtgc aatatcaacc tgatcgtgcc aggaccattc acactccagt
    16021 cccagttcat gttcgacatg cttaccaaaa tgctgcaatt gcggcaggtc atcgtgcgtc
    16081 agggcgaact tccatacggc gttaacttgt tgcgccgttt cagccagaga agcgacttgt
    16141 gtgaaagtcg gacgctgttc cggcggcagg gtttgcgccc agttagacgt gcgaatgaca
    16201 tgcccggtcg ggtgctcata gaccattgca tcatccacat acatcagacc gtgaatgtgg
    16261 tgttcattca gcatctcaat gagttgcagg gctttattaa cgggcattgg gtccgcttcc
    16321 agcacggttt ttgcatgata atcatacaaa taggtgccat tacagcaaat agcaggtgta
    16381 tccagcgcca gcgcctgata aaaaggatga atagcgacgt gatggcgacc tgtgacgatg
    16441 attaattgat agcctgcttc gcgagcgcgg gccagggctt ctatcgatga aggaagcagg
    16501 gtctttttcg gggtcaataa ggtgccgtct aagtcgagag caatcacgcg tgtggtcatg
    16561 gcgtattcca gattaaggtt aagaattttc gtctgcgcga atggtacacc gataccacta
    16621 tcggacaaaa ttctgcgttt taattcagca ttcaccgcca aaagcgacta attttagctg
    16681 ttacagtcag ttgctaaatg caaaggagca ttcatgaagc aaacagttta tatcgccagc
    16741 cctgagagcc agcaaattca cgtctggaac ctgaatcatg aaggcgcact gacgctgaca
    16801 caggttgtcg atgtgccggg gcaggtgcag ccgatggtgg tcagcccgga caaacgttat
    16861 ctctatgttg gtgttcgccc tgagtttcgc gtcctggcgt atagtatcgc cccggacgat
    16921 ggcgcactga cctttgccgc agagtctgcg ctgccgggta gtccgacgca tatttccacc
    16981 gatcaccagg ggcagtttgt ctttgtaggt tcttacaatg cgggtaacgt gagcgtaacg
    17041 cgtctggaag atggcctgcc agtgggcgtc gtcgatgtgg tcgaggggct ggacggttgc
    17101 cattccgcca atatctcacc ggacaaccgt acgctgtggg ttccggcatt aaagcaggat
    17161 cgcatttgcc tgtttacggt cagcgatgat ggtcatctcg tggcgcagga ccctgcggaa
    17221 gtgaccaccg ttgaaggggc cggcccgcgt catatggtat tccatccaaa cgaacaatat
    17281 gcgtattgcg tcaatgagtt aaacagctca gtggatgtct gggaactgaa agatccgcac
    17341 ggtaatatcg aatgtgtcca gacgctggat atgatgccgg aaaacttctc cgacacccgt
    17401 tgggcggctg atattcatat caccccggat ggtcgccatt tatacgcctg cgaccgtacc
    17461 gccagcctga ttaccgtttt cagcgtttcg gaagatggca gcgtgttgag taaagaaggc
    17521 ttccagccaa cggaaaccca gccgcgcggc ttcaatgttg atcacagcgg caagtatctg
    17581 attgccgccg ggcaaaaatc tcaccacatc tcggtatacg aaattgttgg cgagcaggga
    17641 ctactgcatg aaaaaggccg ttatgcggtc gggcagggac caatgtgggt ggtggttaac
    17701 gcacactaac cgctgattta accggcgcag tctctcctgc gccggtgtat taacctatct
    17761 cctgtaacgc atgtctctgg cgttcgacga tattggtcca caaattgtct ttatcgtcag
    17821 tccacaaatt aatcagcaag gcaaaaaagc gttctgctgc cggagaaagc acggcatctt
    17881 tgcggcgaat aatccccaat gtccgacgta taacgggttc caccagtgga ataccaataa
    17941 tcgaagaata gggcgcatgg ggcattgcca gaccaggaag cgccgaaatc cccagtcccg
    18001 cctctaccag tcctaatgac gtcgaaagat gacgcacttc gtaaaaccag tccagcttcc
    18061 agggcttgtc ggctagctgt tgttctatca acaatcggtt gccgctggag gaacgtacgc
    18121 caatcatttt gtaacccact aactcctgcc attctacaag ctgctttttg gccaatggat
    18181 ggtcacgccg acaggccagc acgaatggct cgttgaccag tggggtaaaa tcaatggatg
    18241 aatttgtgac gttgttcatg tttatgccaa agtctgattc gttacacagc accgattcca
    18301 tgcaattatt cgtgccttgt tccagaatcc gcactttaat attgggatac agctcattaa
    18361 atttaccgat tgccagcggt aaaaaataaa atactgcggt cggaatacac gctaacgtca
    18421 ccataccacg atgataagca ttcatatcac gaatattaaa aagcgtttca tcgaatttct
    18481 ttattaattc ccgcgcctcc gggagcaacc tttttcctgc ttttgttaac gtaaccttgc
    18541 gagttgtacg ctcaaatagt tgaatatgta aatcttcctc catctttttt attcttcgcg
    18601 ttaatgcagg ttgcgtaata ttgagtaatt tagcagcatt gttaaatgaa ctggactctg
    18661 ccagtatgac aaatgccttc atacttgata attcatgctt cattattact ccggaaaatg
    18721 gaagcgacga ttttgggtgg ctggtcgtta aaaattttaa ctgcatttag ccaacttaaa
    18781 ttaatgaaaa aatgttatta atcgttgagc taaagtcatt agagatgctt tgtccttaat
    18841 gtaaccatat cgcaataagt tatgttttta aattgagggc attattatga aaaaaatacc
    18901 ctgcgtgatg atgcgaggtg gaacctcgag gggcgcgttc ctgttagcgg aacatttacc
    18961 cgaagatcaa acgcagcgcg ataaaatatt gatggcaatt atgggttccg gtaacgatct
    19021 ggaaattgac ggtattggcg gcggtaatcc actgaccagt aaagtcgcca ttattagccg
    19081 ttccagcgat ccgcgtgctg atgttgatta tctgtttgct caagttatcg tccatgagca
    19141 acgtgtcgat accacgccta actgcggcaa tatgctttct ggcgttgggg cattcgccat
    19201 tgaaaatggt ttgattgcag cgacatcacc ggtcacccgt gtgcgtatcc gcaacgtcaa
    19261 tacgggtacg ttcatcgaag ctgatgtgca aacgccaaat ggtgttgtcg agtacgaggg
    19321 tagtgccaga attgacggcg taccgggtac tgccgcaccg gttgcgctca ctttcctgaa
    19381 tgccgctgga accaaaaccg gaaaagtttt cccgactgat aatcagattg attattttga
    19441 cgatgtcccg gtgacctgta tcgatatggc gatgccagtc gtcattattc cggctgaata
    19501 tctgggaaaa acaggttatg aattaccggc ggaactggat gccgacaaag cattattagc
    19561 ccgcattgaa tctatccgtc tacaagcggg taaagcaatg ggcttaggtg atgtcagtaa
    19621 tatggttatc cctaaacctg tgcttatttc tccagcgcag aaaggcgggg caattaatgt
    19681 gcgttatttt atgccgcatt cttgccatcg cgcgctggcg ataaccggtg ctattgctat
    19741 ttccagtagt tgtgcattgg aaggcaccgt cacccgacaa atcgtccctt ctgtaggata
    19801 cggcaatatc aatattgaac accccagtgg tgcgctcgac gttcatttaa gtaatgaagg
    19861 tcaggatgcc acgacgttac gcgcatctgt tattcggacg accagaaaaa tattttccgg
    19921 tgaagtttat cttccctgaa aaaattcgtt gtcaagataa ggataatcaa taaaggactt
    19981 ctgtatgagt catacagaaa gaacaggatt ttaaatgaat aagaaatcgt tatggaagct
    20041 aattctgata ttagcgatcc catgtattat tggttttatg ccagctccgg caggattaag
    20101 cgaactggcg tgggtgcttt ttggtattta cctggcggcc attgtggggc tggttatcaa
    20161 gcctttcccg gaacctgtcg tactgttaat tgccgttgct gcctccatgg tggtggtcgg
    20221 taacttatcc gacggtgcgt ttaagaccac cgccgtatta agcggttact cttcaggtac
    20281 tacctggctg gtgttctcgg cgtttacctt aagcgccgca tttgtgacca ccggtttagg
    20341 taaacgtatt gcctatctgc tgattggtaa aatcggtaac accacgctgg gtctgggtta
    20401 cgttacggta ttcctcgatc tggtactggc tccggcaaca ccgtctaaca ccgcgcgtgc
    20461 gggcggtatt gtgttaccga tcatcaacag cgtggcggtg gctttggggt ccgaaccgga
    20521 aaaaagtccg cgtcgtgtcg gacattacct gatgatgtcc atttacatgg tcaccaaaac
    20581 caccagctat atgttcttta ccgcaatggc ggggaacatt ctggcgctga aaatgatcaa
    20641 cgacattctg cacctgcaaa ttagctgggg tggatgggca ctggccgccg gattgccggg
    20701 catcattatg ctgctggtca ccccactggt gatttatacc atgtatccgc cagaaattaa
    20761 gaaggtggat aataaaacca tcgctaaagc gggccttgcc gaactgggac cgatgaaaat
    20821 ccgcgaaaaa atgctgctcg gtgtctttgt gctggcgcta ctgggctgga ttttcagtaa
    20881 gtctctgggg gttgatgaat ccactgtggc aatcgttgtt atggcgacca tgctgctgct
    20941 gggtatcgtt acctgggaag acgtggttaa aaataaaggc ggctggaata ccttaatctg
    21001 gtacggcggt attatcggat taagctcctt attatcgaaa gttaaattct tcgaatggtt
    21061 agctgaagtc tttaaaaata acctggcatt tgatggtcac ggtaacgttg ctttcttcgt
    21121 tattattttc ctcagcatta tcgtgcgtta tttcttcgct tccggtagtg cctatatcgt
    21181 tgctatgtta ccggtatttg ccatgctggc gaacgtctcc ggcgcaccgt taatgttaac
    21241 cgcgctggca ctgttgttct ccaactccta tggcggcatg gttactcact atggcggcgc
    21301 ggcaggtccg gtcatctttg gcgtgggtta taacgatatt aaatcctggt ggttggtcgg
    21361 tgcggtactg acgatattaa ccttcctggt gcatatcacc ctcggcgtgt ggtggtggaa
    21421 tatgctgatc ggctggaaca tgctgtaaat attctcgtca tacttcaagt tgcatgtgct
    21481 gcgtctgcgt tcgctcaccc cagtcactta ctt
//
LOCUS       DAZGJW010000074        21415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZGJW010000074 DAZGJW010000000
VERSION     DAZGJW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.074"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..362
                     /locus_tag="ACHKIK_002846"
     CDS             <1..362
                     /locus_tag="ACHKIK_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9083987.1"
                     /translation="ILFYLNNNYREKITLEQLSKKFRASVSYICHEFTKEYRISPINY
                     VIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCF
                     AEQEILSEFPQPVSLVG"
     gene            complement(405..1556)
                     /gene="ydiO"
                     /locus_tag="ACHKIK_002847"
     CDS             complement(405..1556)
                     /gene="ydiO"
                     /locus_tag="ACHKIK_002847"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO9083988.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1570..3164)
                     /gene="ydiF"
                     /locus_tag="ACHKIK_002848"
                     /pseudo
     CDS             complement(1570..3164)
                     /gene="ydiF"
                     /locus_tag="ACHKIK_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
     gene            complement(3308..4066)
                     /gene="aroD"
                     /locus_tag="ACHKIK_002849"
     CDS             complement(3308..4066)
                     /gene="aroD"
                     /locus_tag="ACHKIK_002849"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO9083989.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4097..4963)
                     /gene="ydiB"
                     /locus_tag="ACHKIK_002850"
     CDS             complement(4097..4963)
                     /gene="ydiB"
                     /locus_tag="ACHKIK_002850"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO9083990.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4975..6240)
                     /gene="ydiN"
                     /locus_tag="ACHKIK_002851"
     CDS             complement(4975..6240)
                     /gene="ydiN"
                     /locus_tag="ACHKIK_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9083991.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(6467..7681)
                     /gene="ydiM"
                     /locus_tag="ACHKIK_002852"
     CDS             complement(6467..7681)
                     /gene="ydiM"
                     /locus_tag="ACHKIK_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9083992.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGHRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVDSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(7781..8137)
                     /gene="ydiL"
                     /locus_tag="ACHKIK_002853"
     CDS             complement(7781..8137)
                     /gene="ydiL"
                     /locus_tag="ACHKIK_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO9083993.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8273..8380)
                     /gene="rprA"
                     /locus_tag="ACHKIK_002854"
     ncRNA           complement(8273..8380)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKIK_002854"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8566..9678)
                     /gene="ydiK"
                     /locus_tag="ACHKIK_002855"
     CDS             complement(8566..9678)
                     /gene="ydiK"
                     /locus_tag="ACHKIK_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO9083994.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            10067..13123
                     /gene="ydiJ"
                     /locus_tag="ACHKIK_002856"
     CDS             10067..13123
                     /gene="ydiJ"
                     /locus_tag="ACHKIK_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO9083995.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13120..13530
                     /gene="menI"
                     /locus_tag="ACHKIK_002857"
     CDS             13120..13530
                     /gene="menI"
                     /locus_tag="ACHKIK_002857"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO9083996.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13630..13818
                     /gene="ydiH"
                     /locus_tag="ACHKIK_002858"
     CDS             13630..13818
                     /gene="ydiH"
                     /locus_tag="ACHKIK_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO9083997.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14366..14734
                     /gene="sufA"
                     /locus_tag="ACHKIK_002859"
     CDS             14366..14734
                     /gene="sufA"
                     /locus_tag="ACHKIK_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO9083998.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14743..16230
                     /gene="sufB"
                     /locus_tag="ACHKIK_002860"
     CDS             14743..16230
                     /gene="sufB"
                     /locus_tag="ACHKIK_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO9083999.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16240..16986
                     /gene="sufC"
                     /locus_tag="ACHKIK_002861"
     CDS             16240..16986
                     /gene="sufC"
                     /locus_tag="ACHKIK_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO9084000.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYRGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16961..18232
                     /gene="sufD"
                     /locus_tag="ACHKIK_002862"
     CDS             16961..18232
                     /gene="sufD"
                     /locus_tag="ACHKIK_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO9084001.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18229..19449
                     /gene="sufS"
                     /locus_tag="ACHKIK_002863"
     CDS             18229..19449
                     /gene="sufS"
                     /locus_tag="ACHKIK_002863"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO9084002.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19462..19878
                     /gene="sufE"
                     /locus_tag="ACHKIK_002864"
     CDS             19462..19878
                     /gene="sufE"
                     /locus_tag="ACHKIK_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO9084003.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            20027..21031
                     /gene="ldtE"
                     /locus_tag="ACHKIK_002865"
     CDS             20027..21031
                     /gene="ldtE"
                     /locus_tag="ACHKIK_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO9084004.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21094..21330)
                     /gene="lpp"
                     /locus_tag="ACHKIK_002866"
     CDS             complement(21094..21330)
                     /gene="lpp"
                     /locus_tag="ACHKIK_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO9084005.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         5564 a         5221 c         5412 g         5218 t
ORIGIN      
        1 atattctttt ctatctgaat aataattatc gcgaaaaaat cactttagaa cagttatcga
       61 aaaaatttcg tgccagcgtc agttatattt gccatgaatt taccaaagag tatcgtattt
      121 cccctattaa ctatgttatt caacggcgta tgacggaagc aaaatggtca ctcactaata
      181 ctgaattatc acaggcagag atctcctggc gtgtgggtta tgaaaatgtc gatcactttg
      241 ccaaactgtt tttgcgccat gtcggttgtt cgcccagcga ttaccgtaga caatttaaaa
      301 actgttttgc ggaacaagaa atcctatctg aatttcctca accggtaagt cttgttggat
      361 aaatctggcg gcgcagtttg ttaagaagct gcgccgcctg cagattattt gttctgatag
      421 tctttcagga tctgccgacc tgctacgtaa atcataattt cgtctgtacc gccgccgata
      481 cgttcacaac ggacatcacg ccagaagcgg gagacgcgcg cttcatcggt atagcccaga
      541 ccgcccatga tttgaatcgc atcatcaatg acttccattg cggtacgtgc gcaatacagt
      601 tttgccagcg ccgcgctggt gcgcagtgac tgatgctgat cggcttgcca tgccactttc
      661 agcaccatgt tgcgcatgtt gtcgatctta atcgccatca gcgccagttt ttcctggatc
      721 atctggttat gaccaatggg cttaccaaaa gcgatacgtt ggttggcata gcgggcggca
      781 tcttcaaagg cgcattcggc aaagccggtg ctgcgcgcgg cgttgatcag gcgctccatc
      841 tcaaagttgt acatcacatt gaggaaaccc attccttctt cgcccaccat gtcgctctct
      901 tcaacttcca cattgtcgag atagacttcg caggtgctga gcatatgcca gccgatttta
      961 tgcagcgggt taatcttaat gccgggctta ctggagtcga cccaccacag ggtgaaggct
     1021 tttttgggat ctttcggttg cggatcgcgc gccagcacca gcatatacgg gtactctttc
     1081 gcaccggtaa taaaggtttt ttgtccgttg atgtagacct tgccgttttt acgcgtgtaa
     1141 gtggtagtgg cactgttgtt atctgagcca gcacctggtt ccgtcaacgc cagggcatag
     1201 gcgggatcac ccgtttccag ggtgctctct gccgttttac gtagctgctc tgcagaaccg
     1261 aaacggcgca tactgtgaat acattgaccg ttggtaatta aaaatgccgg agcaccgcat
     1321 tttgacactt ccatcagcgc cagcatttgg gtgacgtaat ccgcagggat accaccaaat
     1381 tcttccggca cgccaagcat ggaaataccg ttatccgcca gcgcccgcat aaactcacgc
     1441 ggatatgtcc cgttttgatc gcaggtgcgg aaatactctt ccggaaagtt agtcgtaatc
     1501 agttcgcgaa tactggccag cagcagttct tgttcttcag ttaaagaaaa atccatcatt
     1561 atactcctgt taatgagccg cttcaggcag gacaaaaccc atcgccgcat cgataaataa
     1621 tctttcgtcc atcagtttga gttctggcga aatcactggg gtgaagtcca ttttgtcgag
     1681 aatatctttt tgtaaatcga cgccaggggc gatttcgatt aaatgcaggc cgtcttcttt
     1741 cagcgtgaat acagcacgtt cggtgatgta acgaacatcc agtccccgtt cgagcgcgat
     1801 ttttccgcta aaagtaattt ccggtagttc ccgaataaat ttatttaccc gtccttcctg
     1861 gacgatatgc aatttgccat cggcaatttc tgtttttaaa ctgcccgcag ttaacgtgcc
     1921 gcagaaaata attttcttcg aggtggcact gatatcaata aatccaccgg tacccatgat
     1981 tttgccattg aatttatgca cgccgacgtt accgtgctgg tcgacttcag caaaactcaa
     2041 ataacaaaca tccagaccac caccgtgata aaaatcaaac tgggacgtca tatccagaat
     2101 ggcgcgggta ttcacgttcg cgccaaaggc gataccctgt gaagtaattc cgccaatcgg
     2161 acctgtttct accgtcagaa taaagtcatc agcacaacct tcttcccggg cgaccaggcc
     2221 aataccgtca gcaataccga cgccgacatt ccccaccgcg cctttacgca tttcgaataa
     2281 tgcacgccgc gcaactaatt tacgttggtt taggggcagg ctaagtttgt gctgtcatca
     2341 agggtgaagt caccagaaat aaaacggtta accggcgcac cgccatacag ttggctttga
     2401 tccggatcga ccaccacaat atccaccaga taacccggaa tacggacaga tttaggatgc
     2461 agcgtggctt tcttaaccat tttctgcacc tgcatcatca caataccgcc gttattgtgc
     2521 accgcctggg caataaccaa tgcgtcgaga tacatcacct catcttcaaa agtggcgtag
     2581 ccttcactgt cgcaggtggt agcgcgaatg aaggcgatat ctggcgcaat cgctttgtaa
     2641 tagagatatt ctttgttatc aaactcgacc agtttaatca ggtcttcttt cgtgacttca
     2701 ttcagtttgc cgccttgctg gcgtggatcg acaaatgtcc cgatgccaat atcactaata
     2761 ataccaggct ggtgggctgc ggcggcgcgt aaggtttgtg taagtacacc ttgtggatag
     2821 ttataagcaa taattttatt ttgttctgcg agttcagaaa tacgcggcga ttgtccccag
     2881 tgaccacata atgcccattt caccagacct tcttgcgcca gaggactaat accacggtcg
     2941 gcgcgatcgc caagccctgt cggactaata atcgataaat tacgtggcgt ttgagtctgt
     3001 ttatatttat cagcaagagc tgtaattaac gtggtggctt ccagaatacc gccgccagcg
     3061 cctaacacac aaagtgttgc ttcgtcggga atataattca ccgcttcctg tgccgacagg
     3121 accggcactc gtccattaat acgaggtggt tttacaggtt tcattgttaa ttcctgattc
     3181 agatgtgatt ttccctacgc cagatggaga attagcgcac agagactcac gaaatatttt
     3241 tattaaatac ggttaaatat attttttaag ttcggcgggg agggtgttcc cgccgaaata
     3301 ttattgctta tgcctggtgt aaaatagtta ataccgtgcg taaatcattt accgagattt
     3361 gccctggtgc agaggctttt tttaccgcac caaaagttgc cgccgagcca aatacttcac
     3421 cagccagacg agaaattacg ccagtttttg ccatcgacat cgtgatgatt ggacgatcgg
     3481 catactgctc ctgcatctcc agggtcgcgg taagcaacgt cagcacatcg ctggtacttt
     3541 gcggcatcag cgcaatctta ggaatatcgg cgtcgaagga ctgcattttg cgcagacggg
     3601 caatgatttc ttcggcttcc ggcgttttat ggaagtcatg gttggacatg acaactttca
     3661 catcatgcgc gtgggcgtag gcgacggttt ctttaacctg atcatcgccg gtaaataact
     3721 ccagatcgat catatcaacc aggccgctgt cgatggctgc acgattgaga gcaatataag
     3781 cctcggtgga aatcgcctgc tcgccgcctt ctttggcact gcggaaggta aacagcagcg
     3841 gtttttctgg catggtttca cggagaattt ttgccgccgc catgacagac tccacattgg
     3901 agaggtcggc aaagtggtcc acacgccatt ccagaatatc aaagtccgct tcacgatagg
     3961 cgagagcttc ggatttcacg cgggcgatat ctttcgccat cagcgagacg atgattttag
     4021 gtgcgcccgt accaatgacg agatctttta cagttacggt tttcattttt taccctttct
     4081 gcacgcggtc agcctgtcag gcaccgaacc ccatgacctg tttaacatat tccagaggga
     4141 aatctttgcc agtccataac gtgaactgtt cagccccttg ccacaacaac atgccgtatc
     4201 catcaatcgt tttgcaacca gcttgttgcg cctgctgcaa taacttcgtc atatgcgggt
     4261 tatacacgca ttcagtgacc agcagtcccg gatgtaacag actgatatca ttaactaatg
     4321 attcattctc aaggggtttc atacccactt ttgtgccatt ggttaaaatg tcggcggaag
     4381 ccagggcttc agcaaaggct tgctgatcgg cgagatcggt aaccgtgacg acacaatcgg
     4441 tgttttcgtt aacccgctgc gcgaaggcga gggctttatc gaagaactca tcccgacggt
     4501 taaagagttt aatttctttt aaaccttcaa ttgccccctg cgcgccaatc gccgttgagg
     4561 cacctccggc ccctaacagc accattgttt tgcctttgat atcaaaaccg ctctctttaa
     4621 tggcgcgaat atggcccgtg ccgtcggtgt tatagccgcg caggtagcca tcatcattaa
     4681 cgatggtgtt gatggcaccc accagtttgg ctgctggcgt taattcatca acatattcac
     4741 acgccagttg tttgttcggc attgatatac cagttccgcg cattttgagg gcttttaatc
     4801 cttcaattgc tccgggaaaa ctatcgttat ccacttcaaa ggccatatag gtaaatggca
     4861 atcccgcttt ttctaaggct ttattctgca tttcaggcga taaactgtgg cggataggat
     4921 aggccatcag cccaatcaat tcgtattttg cggtaacatc cataattaac tcctttaacc
     4981 tctatgctta attgtgttta accgggtgct aaatttacgc tcgccaaacc gcacatcatt
     5041 ctcaggaata ataaaaacgc ggtaatagcg gataaaaaca ataattgcgg taatcagggc
     5101 cagcagcgcg aaagtaaaat cgagaacaat gatatattga aggccgatgt tcgacagata
     5161 accggtaatc agcggaataa caaagttagc cagtccaccc atcatcatat aaatactggt
     5221 gactttggct ttgctttttg ggaaaaactc tgacatgacc gaaacaccga gctgtaaaat
     5281 gccgccagct gctgagaaac cgataacaaa ggcgccggca ttacacacca gtggagaagg
     5341 gtacaggtag ataatggctg ctgttattgt tgccagtgca gagttaaata cattagccca
     5401 gatgggccgg accatttttt tcagtagtgc ggcaaaaata aagacacaga ccaacgagcc
     5461 catactgtaa taagagatgg tttttaatgc ctcagcttct gacataccag caaaagccat
     5521 cgcatatttg ggcatccaca ccacaatcac ataaaaggtc gagaatgcgg ctacaccgaa
     5581 cagtaccgat gaaacacctt ccagccagac taacggtttg ctgttcattt gcggtaattc
     5641 attagctacg ctggcgtcca ccaactggct ggggaatttg cttttcaaca gcatcagcgt
     5701 gatcagtaca aacagaatac ccggaataat cagcccatag ccgtaccaga tattattgag
     5761 caacatatag ctcaccagca ttgggtagaa catttgccca aatgacacca tcgctttaac
     5821 cagtatgacc gccgaaccag aggctttcgg aaagcattcc atgagcgcgg ggtagccacc
     5881 cgtatccagc gctgagttag cgatacctac gcacactgcc agaccgtagg cgagagttaa
     5941 attcgggcaa gcgggaatac caaagaagaa tagcagatac attattactg ccattaatat
     6001 caccgcccga cgaccaaact tatcggagat cacaccgaag aataaaatac tgatcaatcg
     6061 ccccaaacca ataccggaaa ttaagtaggc aatgcccgcg ttgtcagtgg aaaacttttc
     6121 cgccagagat gacatgtttt gggcaagcgt aataacacta atgccgtgca ggaagtagct
     6181 gaagtaaata caaagaacag ccaggataaa tggcgtgctg aaagccttat tttgagacat
     6241 tttcacagct ccttgctgaa agataatttc gttaccgtac gtattgttct tcgacggttt
     6301 acataattac tatactgaag atgattttcc ctgggtacat ggcataaagt ttatgtttcc
     6361 aggaatattg catgtcatag tttgtttttg atcgcgattt tccttagtta gcgcagaaag
     6421 agttatttat ccgtaagatc ttgcatgacc tgaaaagaat attgcattac ccacccggag
     6481 cgacattttc ctttagcgag tgatgccgcg tttttttgcg gctgcgtaag ccgataaaca
     6541 gtgccagtaa aaaaccgatg gcagcgatgg cggtatcgaa ccacataata tcggcaatac
     6601 ttctttggga cagatgagcc gtaatcaacg gaatagtaaa ggtcgcaata ctgcccgcac
     6661 tgtaatagat ccctgtagct ttacctttag cgtaagggaa acgttcagcc attaacgtca
     6721 ggccaatttg cacaacacct ccagcagagg taaaaccaat gacaaaagca aatattatca
     6781 ccacataaaa tgtgggatgc aggcagacgg taaacagagc aataaatgag ataaaggtgt
     6841 acagcatcag taatgttgtt gggcgaacgg tattacgaat gagtggagcg gtaataaata
     6901 cacaaagcag cgaatccacg gtgtagatac tgagtagttt aatcgacata gtgtatgaca
     6961 tgcctgcaac aaattgtccg tactgtgcca gccactggct aaccagataa aacgttgcca
     7021 ttgagatata gccatataag gtataactgg ctaaatcgat aattgaacag cgatgttccg
     7081 tagagctggt ggttttcttt atgacaggta agcgatgacc cggatggggt gggaacgtac
     7141 aacggtataa aaacagagcg ttaataaaca taatgcctgc agcaatcata aaggaccaac
     7201 cgaaccacag ttcagcccac actaacaggc taatgattag cggtaataaa aattgtccgc
     7261 tggaaacaaa tgctttaatt aaaatattgg ctgtcccagg tgagcgtgga aaagcttcca
     7321 tcaaactggg ataagtgcct gcatcgagaa aactgtttgc cattcctgcc agaaagccaa
     7381 aaacataagc gataatgatg ttattggtgt gcaggatgcc aaaaaagaag gccatatagc
     7441 agcacatccc gagcatgata aaagggcggc gaccaaagcg atcggataat aatcctgcaa
     7501 aaagcaagac acttaatcga ccaatgccca gcgatgagat aactatcgag acacccgcgg
     7561 cattagtctg ccaaagtgtc tccagcgagg ccatattcag gctcatcaaa atgacgccca
     7621 taccatgcac taggtaatta aaatacaacc caagtgcggt agggtaatag ggatttttca
     7681 taggttctac ttagcaaagg tgctttaaaa agtgtagcga tgttgtgtca gtatccgttc
     7741 attattgttg taacaacgac aattcctttt tgtggagaaa ttaacaaagc cgctccagat
     7801 cgtgtgcata taattcagcg gctaccgatt gatagatctt ccagtcaata aaattgccat
     7861 cagggtagac ttgttgaaac gcggttaagt cggggaaaaa acgcatggtg ttgttgccga
     7921 tgcggttatt tattttctct atgatagcat gaagatgttt ttttcgttgc tgacgcatag
     7981 ccaacagttg ctcgactaca cgatcaggaa tggcgcattt gccattttcc cactgacgcc
     8041 agctttcact gtcaccggtc tgggcaatcc aggtcgcgca ttcatcaata gtcatggcaa
     8101 aaatatggcg aagtgcctgg agttcataag cgttcatcgt gtaatggctg catgaaataa
     8161 acaataaaat ataaataaca ttatcagcct gctgacggct tgaagagagt cacagtatct
     8221 tgtgcaacat tattggaaag agtgaggggc gaggtagcga agcggaaaaa tgttaaaaaa
     8281 aagcccatcg tgggagatgg gcaaagacta cacacagcaa ttcgttgttt cactcagggg
     8341 atttccatgc ttataaatca atatgttgat ttataaccgt aagctaatag taggcatacg
     8401 gactttttgc gtcgatcaga ttcgtctcat tagtttaaaa actgtaaaga atttgcgaca
     8461 ggtactacca gctgaatggc ggtaagcagg aaaatatggg gaagagcaag caaatatcaa
     8521 ttagcggggg ttaacaatca ggcggcagag ccgcctgatg agaaattact tattcggttt
     8581 ctcgatttcg ccaagttctt cgagaatttc ttccggttgg tccgtcggcg gcgggacttc
     8641 ttccacccac gcggcaaaca gacgccagga aacggctaac agaaccggac caataaacag
     8701 accgatcatc ccgaaagcaa tcaaaccacc aataacacca gagaggatca ggatcagcgg
     8761 taaatcggca cccatgcgaa ttaacattgg gcggatgacg ttatccagcg tgccaaccac
     8821 accgctccac actaacaata ccgttcccca ggtggtatcg ccagtccagt agagccagat
     8881 aatcgccgga atcagtaccg gcaacgggcc aagctggaca aggcaggaga ggatcattaa
     8941 caccgttagc aacgttgcat aaggtacgcc ggatacggcg aggccgatac cgccaagcac
     9001 tgcctgtact aacgccgtca ccaccacacc cagcgccacc gcgcggatag cctgtgccgc
     9061 cagcagtacg gcggcatctc cgcgaacgcc tgccagacgg gttgcaaaat ggcgaatgcc
     9121 ttgtgccacc tgttcaccgc gccagtacag cagggcactg aagagaagca tcagcgcaca
     9181 atgcaccata aagcgcccga tatgcgccgc ctgcccaacg aaccaggtgg tggtggtgcc
     9241 aatataaggg cggactttcg ccatgatcgc cgtccccccc atatccagca agttgtgcca
     9301 gcctgcatac agcttcgcgc caatcaccgg aatggtatta agccacgcca gatcgggtaa
     9361 cgtcatgtcg ccgctggaaa tggctttaat tagcgggccg ctgccgtcga cgatactgtt
     9421 aaccagcaaa gcaataggga tgataaacac catcactaat aacagcgtca tcaccagaac
     9481 ggcgagagag cggcggccaa acatgatctt ttgcaaacgt aacaataccg gccaggtggc
     9541 gataaccacc gtaccggccc atgcaaagcc gagaataaag ggttgaacaa tccacagaca
     9601 tgccacaatc atgatggcta aaaacagcac cgaaagcaga atttgtgcga catccctggg
     9661 ctgacgaaca tttaccataa ctacttttca ccttttttgt gcgtcaaatc gtcgacgcga
     9721 cgtgaacacg cgaaactcac ctgcataatg atgagcaatt tcagcggttt ttaacaggcc
     9781 gattctgccc ttaattctga tgggcgtgtt agaaaaaaat gtggtacaac aactcaggca
     9841 acacgcaaac gatttactcg tcgtatttca agttgcatgt gctgcggcta cgttcgttca
     9901 ccccagtcac ttactattgt aagctcctgg agttcactcg cttgccgcct tcctgcaact
     9961 cgaaatccat agagtaaata aacatcacct ttcagtgcgc tattgcagcc gcctgaaaga
    10021 atagtgtatc gacctacatc acagaccgca ggaaagggtc aatataatga ttccacagat
    10081 ttcccaggca cccggcgtcg ttcaactggt gcttaatttt ttgcaagagc tggagcaaca
    10141 aggttttacc ggcgatacgg cgacaagtta tgccgatcgt ctgacaatgt cgaccgacaa
    10201 cagtatttac caacttctcc ccgatgcggt ggtatttccg cgttcaaccg cagatgtggc
    10261 gctgatcgcc cgtcttgccg cgcaggaacg ctattcatcg ctgatcttta ccccccgtgg
    10321 cggcggcacc ggcactaacg gtcaggcgct caaccagggg attattgttg atatgtcccg
    10381 ccatatgaac cgcatcatcg aaattaaccc tgaagagggc tgggtgcgcg ttgaggccgg
    10441 ggtgataaaa gatcaactca atcagtacct gaaaccgttc ggctactttt ttgcgccgga
    10501 actttcgacc agcaaccggg caacgctcgg tgggatgatc aataccgatg cttctggtca
    10561 gggatcgctg gtctatggca aaacgtcgga tcacgtactt ggcgtacgtg cggtgttgtt
    10621 ggggggcgat attctcgata cgcaaccttt acccgtcgaa ctggcggaaa cgctgggtaa
    10681 atccaatacc acaatcgggc gaatttataa cacggtttat caacgttgcc gtcagcaacg
    10741 ccagttaatc atcgacaact tccccaaact taaccgcttt cttaccggtt acgatctgcg
    10801 tcatgtcttt aacgatgaga tgaccgagtt cgacctgacg cgcattctga cgggttcaga
    10861 agggacgctg gcctttatta ccgaagcgcg gctggatatt acgcccttgc ctaaagtgcg
    10921 ccgtctggtg aacgtcaaat atgactcttt tgactccgcg ctgcgtaacg cgccgtttat
    10981 ggttgaggcg cgggcgcttt cggtagagac ggtggactca aaagtgctga atctggcgcg
    11041 ggaagatatt gtctggcatt ccgtcagcga gttgattacc gatgtgcctg acaaagagat
    11101 gctcgggctg aacattgtgg agtttgctgg tgatgatgag gcgctgattg acgagcgagt
    11161 taatgctctc tgtgtgcggc ttgatgagct gattgccagc caacaggcag gtgtgattgg
    11221 ctggcaggtg tgccgcgagc tggcgggtgt tgaacgtatc tatgcgatgc gtaaaaaagc
    11281 cgttggtctg cttggcaatg ccaaaggtgc cgctaagcca attccgtttg ctgaggatac
    11341 ctgcgtaccg ccggaacatc tggccgatta tattgctgaa tttcgcgcgc tgctcgacag
    11401 ccacggctta agctacggta tgttcggtca cgtcgacgca ggtgtcttgc acgtccgtcc
    11461 agcgctggat atgtgcgatc ctcaacaaga gattttgatg aagcaaatct ctgatgacgt
    11521 agtggcgctg actgcgaaat acggtggttt gttgtggggc gagcacggca aaggttttcg
    11581 cgctgaatac agcccggcgt ttttcggtga ggaacttttt gcagaactgc gcaaagtgaa
    11641 agcggcattt gacccgcata accgactcaa cccagggaag atttgcccgc cagaaggtct
    11701 cgatgcgccg atgatgaaag tggacgcggt gaagcgcggt acattcgatc ggcagatccc
    11761 cattgcggta cgccagcagt ggcgcggtgc gatggagtgt aacggcaacg gtttatgctt
    11821 caactttgat gcccgtagtc cgatgtgtcc gtcgatgaag atcacccaga accggattca
    11881 ttcaccgaaa gggcgcgcaa cgctggtgcg tgaatggctg cgtttgttgg cggatcgcgg
    11941 cgttgatcca ctcaaactgg aacaagaact gcctgaatcc ggcgtcagtt tgcggacgtt
    12001 aattgcccgc acgcgcaata gctggcatgc gaataaaggc gaatatgact tctcacacga
    12061 agtcaaagag gcgatgtcgg gctgtctggc ctgtaaagcg tgttcgaccc agtgccccat
    12121 caaaattgat gtgccggagt ttcgctctcg ttttctgcag ctctatcaca cccgttattt
    12181 acgcccgctg cgcgaccacc tcgtcgctac ggtcgagagc tacgcgccgc tgatggcacg
    12241 cgcgccgaaa acctttaact tcttcattaa ccagccgctg gtgcgcaaac tctcggaaaa
    12301 acatatcggc atggttgatt tgccgctgct gtcggtcccc tcgctacaac aacaaatggt
    12361 ggggcatcgc tcggcaaaca tgacgctgga acagcttgaa tctctcaatg cagagcagaa
    12421 agcgcgcaca gtgttggtgg tgcaggaccc ctttaccagc tattacgatg cgcaagtggt
    12481 ggcggatttt gtccgtctgg tcgaaaaatt aggtttccag cctgtgttac tgccattttc
    12541 gccaaatggc aaagcccagc atattaaagg ttttcttaat cgttttgcga agacggcgaa
    12601 aaagacggcg gatttcctca accgtatggc gaagctgggt atgccaatgg tgggcgtcga
    12661 tccggcgctg gtactttgtt atcgcgatga atataaactg gcgctgggcg aggaacgtgg
    12721 cgagtttaac gtcttactgg cgaatgaatg gctggcaagc gcacttgagt cacagccagt
    12781 ggctacagtc agcggtgaat catggtattt ctttggtcac tgtaccgaag ttaccgcctt
    12841 gccgggtgcg ccagcacaat gggccgcgat atttgcccgt tttggcgcga aactggaaaa
    12901 tgtcagcgtg ggttgctgcg gcatggcagg gacttacgga catgaagcga aaaaccataa
    12961 aaattcgctc gggatctatg agttatcctg gcatcaggct atgcagcgac tgccgcgtaa
    13021 ccgctgtctg gcgaccggat attcctgccg tagccaggta aaacgggttg aaggcacggg
    13081 ggtacgccat cctgtgcagg ctttactgga gattattaaa tgatatggaa acggaaaatc
    13141 accctggaag cactgaatgc tatgggtgaa ggaaacatgg tggggttcct ggatattcgc
    13201 tttgaacata ttggtgatga cacccttgaa gcgacaatgc cagtagactc gcggacaaag
    13261 cagcctttcg ggttgctgca tggaggagca tccgtggtac tggccgaaag tatcggttcc
    13321 gttgccggtt atttatgtac cgaaggtgag caaaaagtgg ttggtctgga aatcaatgct
    13381 aaccacgtcc gctcggcacg agaagggcgg gtgcgcgggg tatgcaaacc gttgcatctc
    13441 ggttcgcgtc accaggtctg gcagattgaa atcttcgatg agaaagggcg tttgtgctgt
    13501 tcgtcacgat tgacgaccgc cattttgtga cgttgtcacc agaaaagtgt gacgcatatt
    13561 gcctttggtc attatctgca cagtaaacgg tggtatactg tgcaggtctt gtctaaaagc
    13621 gaggatgcta tgtctactca acttgatccc acccagctgg ccattgaatt tttacgccgt
    13681 gaccagagca atctttcacc tgcccaatat ctcaaacgtc tgaaacaact agaacttgaa
    13741 tttgctgacc tgcttactct ctcttctgct gaattaaaag aagaaatcta ctttgcctgg
    13801 cgtctgggcg tgcattgatc ctgacttaat gccgcaagtt cgccaaatcg acctggatta
    13861 acctccgtcc aggttcgatg accttcttaa tactgcttat ctaccgcctg atattgttca
    13921 aaaataatac taatcgcagt ttgtgttaaa acggcgggtt agctttatga gattattctt
    13981 atcgcccctt caagagctaa gccactgaga gtgccggaga taagcgccgg atggggtaga
    14041 aacccttaag cctgtgttgc acagacttaa gggttttctt atttctgaac gctgcacttt
    14101 tttacctatc tcttaaacag tcattttcaa tacaactttt tccttttcat cacagcctta
    14161 atgctcctta gataataacc attatctaac aatgagatac ctaattctta gcgtgcctgt
    14221 taacccacac atcagggtct atgcttatta aaagcaggca aaaagcatgt tttacggtaa
    14281 agcccctgcg tttgctgggt tgtactgata atcattatca ctaacatgct gttatacgct
    14341 gaaagcgatg aagtgaggta aatcgatgga catgcattca ggaaccttta acccacaaga
    14401 tttcgcctgg caaggcttaa cgctgacacc cgcagcggcg atacacatcc gtgagctggt
    14461 ggcaaagcag ccgggtatgg tcggcgtgcg cttaggcgtg aagcaaacgg gctgcgcggg
    14521 ctttggctat gtgctcgaca gtgttagcga gccggacaaa gacgatctgc tgtttgaaca
    14581 cgacggcgcg aagctgtttg tcccgctgca agcgatgccg tttattgatg gcacggaagt
    14641 cgatttcgtt cgtgaaggac ttaatcagat attcaaattt cacaacccta aagcccagaa
    14701 tgaatgtggc tgtggcgaaa gctttggggt ataggcggta ctatgtctcg taatactgaa
    14761 gcaactgacg atgtcaaaac ctggaccggc ggcccgctga attataaaga aggattcttc
    14821 acccagttag ccaccgatga gctggcaaag gggataaacg aagaggtggt gcgcgcaatt
    14881 tcggcgaagc gtaatgagcc ggagtggatg ctgaagtttc gtctaaacgc ctatcgcgca
    14941 tggctggaga tggaagaacc gcactggttg aaagcgcact acgacaagct gaattatcag
    15001 gattacagct actactcagc accatcgtgc ggtaattgtg acgacacttg cgcgtctgaa
    15061 cctggcgcgg tgcagcaaac tggcgcgaac gcctttttaa gtaaagaggt ggaggcggcg
    15121 tttgagcagt tgggcgttcc cgtgcgggaa ggcaaagagg tggcggtgga tgccattttc
    15181 gactcagttt cggttgccac tacttatcgc gaaaaactgg cggagcaggg aattattttc
    15241 tgttcctttg gtgaggcgat ccacgatcac ccggaactgg tgcgtaaata tctcggcacc
    15301 gtggtgccgg ggaatgacaa cttctttgcc gcgcttaatg cggcggtagc ctctgacggt
    15361 acgtttattt atgtgcctaa aggcgtgcgc tgcccgatgg aactttccac ctattttcgc
    15421 attaacgcgg aaaaaaccgg gcagtttgag cgcaccattc tggtggccga cgaagacagc
    15481 tacgtcagct acattgaagg ctgttccgct ccggtgcgtg acagctatca gttacacgcg
    15541 gcagtggtgg aagtcatcat ccataaaaac gccgaggtga aatattccac ggtacaaaac
    15601 tggttccctg gcgataacaa caccggcggt attctcaact tcgtcaccaa gcgtgctttg
    15661 tgcgaaggcg aaaacagcaa aatgtcatgg acgcaatcag aaaccgggtc cgcgattacg
    15721 tggaaatatc ccagctgcat tttgcgcggc gataactcca ttggtgagtt ttactcagtg
    15781 gcgctgacca gcggtcatca gcaagcggat accggcacca agatgatcca catcggtaaa
    15841 aacaccaaat cgaccattat ctcgaaaggg atctctgccg gacatagtca gaacagttat
    15901 cgcggcttag tgaaaatcat gccgacggca accaatgcgc gcaatttcac tcagtgcgac
    15961 tcaatgctga ttggcgctaa ttgtggggcg cataccttcc cgtatgtcga atgtcgcaat
    16021 aacagcgcac aactggagca cgaggcaacg acatcacgta ttggtgaaga tcaactgttt
    16081 tactgcctgc aacgcgggat cagcgaagaa gacgccatct cgatgattgt taacggtttc
    16141 tgcaaagacg tgttctcaga actgccgctg gaatttgccg ttgaagcaca aaaactcctc
    16201 gccatcagtc ttgaacacag cgtcggataa ggaataaaca tgttaagtat taaagattta
    16261 cacgtcagcg tggaagataa agctatcctg cgtggattaa gcctcgccgt tcgtcccggc
    16321 gaagttcacg ccattatggg gccaaacggt tcgggcaaaa gtaccttatc ggcaacgctt
    16381 gccgggcgcg aagattatga agtgacgggc ggcacggttg agttcaaagg caaagatttg
    16441 cttgcactgt cgccggaaga tcgtgcaggc gaaggcatct ttatggcctt ccagtatccg
    16501 gtggagattc caggcgtcag taaccagttt ttcctgcaaa cggcacttaa tgcagtgcgc
    16561 agctatcgcg gccaggaaac gctcgaccgc tttgattttc aggatttgat ggaagagaaa
    16621 atcgctctcc tgaagatgcc ggaagattta ttaacccgtt cggtaaacgt tggtttttcc
    16681 ggcggcgaga aaaagcgcaa cgatattttg caaatggcgg tgctggaacc ggagttatgc
    16741 attcttgatg agtcggactc cgggctggat attgacgcat taaaagtggt cgccgatggc
    16801 gtgaactcgc tgcgtgatgg caagcgctca ttcatcattg ttacgcacta ccaacgcatt
    16861 ctcgactaca tcaagcctga ttacgtccat gtgctatatc ggggacgaat tgtgaaatcc
    16921 ggcgatttca cgttggtcaa acaactggag gagcagggtt atggctggct taccgaacag
    16981 cagtaacgcg ctgcaacagt ggcatcactt gtttgaagct gaagggacaa aacgctcacc
    17041 gcaagcacag cagcatttac aacaattgct gcgtaccgga ctgccgacac gtaaacatga
    17101 aaactggaaa tatacgccgc tggaagggct gaccaatagc cagtttgtca gcattgcggg
    17161 agagatatcc ccacagcagc gagatgcctt agcgttaacg ttagacgctg tgcggctggt
    17221 atttgtcgat ggacgttacg tgtcagcact gagcgatgcg actgaaggca gcgggtatga
    17281 agtgagcatt aacgacgacc gtcagggggt acccgacgct attcaggcgg aagtgtttct
    17341 gcatttgacg gaaagcctgg cacaaagcgt gacgcatatc gccgtgaagc gcggtcaacg
    17401 gccggcaaag ccattgctgt taatgcatat tacccagggc gtggcaggtg aagaggtcaa
    17461 cactgcccat taccgacatc atctggatct ggcggaaggt gccgaagcaa cggtgatcga
    17521 acattttgtc agccttaatg atgctcgtca cttcaccggc gcacggttca ctatcaatgt
    17581 cgcagcgaac gcccacttgc agcatatcaa gctggcgttt gaaaacccgg tcagtcacca
    17641 ctttgcccat aacgatttgt tgctggctga cgatgccacc gcatttagcc atagtttcct
    17701 gctgggtggc gcagtgttac gacacaacac cagtacgcaa ctcaatggcg aaaacagcac
    17761 gctgcggatc aatagcctgg cgatgccggt gaaaaacgag gtgtgtgata cccgtacctg
    17821 gctggaacac aataaaggtt tttgtaacag ccgacagttg cacaaaacta tcgtcagcga
    17881 caaaggccgc gcggtattta acggtttgat caacgtcgcg cagcacgcca tcaaaacgga
    17941 tggtcagatg accaacaata atctgctgat gggcaaactg gcagaagtgg atacgaaacc
    18001 gcagctggaa atctatgcag atgatgtgaa atgcagccac ggcgcgacgg tggggcgtat
    18061 tgatgatgaa cagatgttct atctgcgctc gcgcgggatc aatcagcagg atgcccagca
    18121 gatgatcatt tacgcctttg ctgccgaact gacggaagca ctgcgtgatg aggggcttaa
    18181 acagcaggtg ctggcccgaa tcggtcaacg gctgccagga ggtgcaagat gactttttcc
    18241 gtcgacaaag tgcgggccga ctttccggtg ctttctcgtg aggtaaacgg tttgccgctg
    18301 gcttatctcg acagcgccgc cagtgcgcag aaaccgagcc aggtgattga cgccgaggcc
    18361 gagttttatc gtcatggcta cgcggcggtg catcgcggta ttcatacctt aagcgcccaa
    18421 gcgaccgaga aaatggagaa cgtgcgcaag cgggcatcgc tgtttattaa tgcccgttcg
    18481 gcggaagagc tggtgttcgt ccgcggcacg acggaaggga tcaatctggt cgccaatagc
    18541 tggggtaaca gcaatgtgcg ggcgggcgat aacatcatca tcagtcagat ggagcaccac
    18601 gctaacattg ttccctggca gatgctttgc gcacgcgttg gcgcagagct gcgtgtgatc
    18661 ccgctcaatc ccgacggtac gttgcaactg gagacgctgc ctacgctgtt tgatgagaaa
    18721 actcgcctgc tggcaattac tcatgtctcc aacgtgcttg gcacagaaaa tccactggcg
    18781 gaaatgatca cgcttgcgca ccagcatggc gcaaaagtgc tggtggatgg cgctcaggcg
    18841 gtgatgcatc atccggtgga tgttcaggcg ctggattgcg acttttacgt gttctccggg
    18901 cataaactgt atggccccac cggaattggc attctttatg tgaaagaagc cttgttgcag
    18961 gagatgccgc cgtgggaagg gggcggttct atgatcgcca ccgtcagcct gagtgaaggc
    19021 acaacctgga ccaaagcacc ttggcggttt gaagccggta cacccaatac cgggggcatc
    19081 attggtcttg gcgcggcgct ggagtatgtt tcggcgctgg ggcttaataa catagccgag
    19141 tatgaacaga atctgatgca ctacgcgcta tcacagctgg aatctgtacc ggatctcact
    19201 ctctatggtc cgcagaacag gcttggcgtt attgctttta atcttggtaa acaccacgcc
    19261 tatgatgttg gcagttttct cgataactac ggcattgctg tgcgtaccgg acatcactgc
    19321 gctatgccat taatggccta ttacaacgtc cctgctatgt gtcgggcgtc gctggccatg
    19381 tataacaccc atgaagaagt ggatcgtctg gtgaccggac tgcaacgtat tcaccgtctg
    19441 ctgggataac agggaggcac tatggcttta ttgccggata aagaaaaatt gctgcgtaat
    19501 tttttacgct gcgccaattg ggaagagaaa tatctctaca ttattgagct gggccagcgt
    19561 ctgccagaat tacgcgacga agacagaagc ccacaaaata gcattcaggg ctgccagagt
    19621 caggtgtgga ttgtcatgcg ccagaatgcg cagggaatta ttgaattgca gggcgacagc
    19681 gatgcggcga ttgtgaaagg gcttattgcg gtcgtcttta ttctctacga tcagatgacg
    19741 ccgcaggata ttgtcaattt cgatgtgcgt ccgtggtttg aaaaaatggc gctcacccaa
    19801 catctcaccc catctcgttc acaaggtctg gaagcgatga ttcgcgcaat tcgcgccaaa
    19861 gccgctgcac ttagctaaac taaagggaca gctttcatcc ggttggctca cattgccgga
    19921 tggtcaatgt ttacactgac ctgcacagga gtgtttcggt ttcaggtgaa cataagcgcc
    19981 atccgacgtt tcagcgtgag tctccggcaa atacaggagg tttacaatga aacgcgcgtc
    20041 tttgcttaca ctcacgctta tcggcgcttt tagcgccatc caggctgcct gggcggttga
    20101 ttatccgcta ccaccaaccg gaagccgact ggttgggcaa aatcaaacgt atacggtgca
    20161 ggaaggggat aaaaaccttc aggccatcgc ccgacgtttt gatactgcgg caatgttgat
    20221 ccttgaagcc aataacacta tcgccccggt gccaaaaccc ggtacgacga taactattcc
    20281 ttcgcaactg ttattacctg atgcgccgcg tcaggggatt atcgttaacc ttgcagagct
    20341 gcgcctttat tattatccgc cgggagaaaa tatcgtgcag gtttatccga taggtattgg
    20401 cttgcagggg ctggaaacgc cggtgatgga aacgcgtgtg gggcagaaaa tccctaaccc
    20461 aacctggacg cctactgcag gcattcgtca acgttcgctg gagcgtggta ttaaattacc
    20521 gccagtcgtt cctgccggac caaataaccc gctaggacgt tacgcactgc gcctcgcgca
    20581 tggtaatggc gaatacctca ttcatggcac cagtgcgccg gacagcgtcg gtttgcgcgt
    20641 cagttcaggg tgtattcgca tgaatgcacc ggatattaaa gccttgttct ccagcgtgcg
    20701 gacgggaacg ccggtgaaag tgatcaacga acctgtgaaa tattccgtgg agcctaacgg
    20761 gatgcgttat gttgaagtac atcgaccact atcggcagaa gaacagcaga acgttcagac
    20821 aatgccatac acactgccag caggctttac gcaatttaaa gacaataagg ctgtagatca
    20881 gaagttagtc gataaagcgt tgtatcgtcg ggcagggtat ccggtttcgg tgagcagtgg
    20941 agcaactccc gcagccagca atgcgccttc agtagagtca gcgcagaatg gtgaaccaga
    21001 gcaagggaat atgttacgcg cgacgcagta gcggtaaacg gcagacaaaa aaatggcgca
    21061 caatgtgcgc catttttcac ttcacaggta ctattacttg cggtatttag tagccatgtt
    21121 gtccagacgc tggttagcac gagctgcgtc atctttagca gcctgaacgt cggaacgcat
    21181 tgcgttcacg tcgttgctca gctggtcaac tttagcgttc agagtctgaa cgtcagaaga
    21241 cagctgatcg attttagcgt tgctggagca acctgccagc agagtagaac ccaggattac
    21301 cgcgcccagt accagtttag tagctttcat tattaatacc ctctagattg agttaatctc
    21361 catgtagcgt tacaagtatt acacaaagtt ttttatgttg agaatatttt tttga
//
LOCUS       DAZGJW010000075        21405 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZGJW010000075 DAZGJW010000000
VERSION     DAZGJW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.075"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(368..1156)
                     /gene="phoH"
                     /locus_tag="ACHKIK_002867"
     CDS             complement(368..1156)
                     /gene="phoH"
                     /locus_tag="ACHKIK_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO9084006.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1775..3046)
                     /gene="efeB"
                     /locus_tag="ACHKIK_002868"
     CDS             complement(1775..3046)
                     /gene="efeB"
                     /locus_tag="ACHKIK_002868"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO9084007.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(3052..4179)
                     /gene="efeO"
                     /locus_tag="ACHKIK_002869"
     CDS             complement(3052..4179)
                     /gene="efeO"
                     /locus_tag="ACHKIK_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO9084008.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVTQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(4237..5067)
                     /gene="efeU"
                     /locus_tag="ACHKIK_002870"
     CDS             complement(4237..5067)
                     /gene="efeU"
                     /locus_tag="ACHKIK_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO9084009.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(5733..7241)
                     /gene="putP"
                     /locus_tag="ACHKIK_002871"
     CDS             complement(5733..7241)
                     /gene="putP"
                     /locus_tag="ACHKIK_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO9084010.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(7400..7609)
                     /locus_tag="ACHKIK_002872"
     CDS             complement(7400..7609)
                     /locus_tag="ACHKIK_002872"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084011.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            7664..11626
                     /gene="putA"
                     /locus_tag="ACHKIK_002873"
     CDS             7664..11626
                     /gene="putA"
                     /locus_tag="ACHKIK_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO9084012.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPLSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(11666..12304)
                     /gene="rutR"
                     /locus_tag="ACHKIK_002874"
     CDS             complement(11666..12304)
                     /gene="rutR"
                     /locus_tag="ACHKIK_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO9084013.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            12592..13683
                     /gene="rutA"
                     /locus_tag="ACHKIK_002875"
     CDS             12592..13683
                     /gene="rutA"
                     /locus_tag="ACHKIK_002875"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO9084014.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            13683..14375
                     /gene="rutB"
                     /locus_tag="ACHKIK_002876"
     CDS             13683..14375
                     /gene="rutB"
                     /locus_tag="ACHKIK_002876"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO9084015.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            14387..14773
                     /gene="rutC"
                     /locus_tag="ACHKIK_002877"
     CDS             14387..14773
                     /gene="rutC"
                     /locus_tag="ACHKIK_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO9084016.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            14781..15581
                     /gene="rutD"
                     /locus_tag="ACHKIK_002878"
     CDS             14781..15581
                     /gene="rutD"
                     /locus_tag="ACHKIK_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO9084017.1"
                     /translation="MKLSLSPPPYADTPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            15591..16181
                     /gene="rutE"
                     /locus_tag="ACHKIK_002879"
     CDS             15591..16181
                     /gene="rutE"
                     /locus_tag="ACHKIK_002879"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO9084018.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            16192..16686
                     /gene="rutF"
                     /locus_tag="ACHKIK_002880"
     CDS             16192..16686
                     /gene="rutF"
                     /locus_tag="ACHKIK_002880"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO9084019.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            16707..18035
                     /gene="rutG"
                     /locus_tag="ACHKIK_002881"
     CDS             16707..18035
                     /gene="rutG"
                     /locus_tag="ACHKIK_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO9084020.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(18118..18291)
                     /gene="ymdF"
                     /locus_tag="ACHKIK_002882"
     CDS             complement(18118..18291)
                     /gene="ymdF"
                     /locus_tag="ACHKIK_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO9084021.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            18664..19260
                     /gene="wrbA"
                     /locus_tag="ACHKIK_002883"
     CDS             18664..19260
                     /gene="wrbA"
                     /locus_tag="ACHKIK_002883"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO9084022.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            19281..19508
                     /gene="yccJ"
                     /locus_tag="ACHKIK_002884"
     CDS             19281..19508
                     /gene="yccJ"
                     /locus_tag="ACHKIK_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO9084023.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(19546..20787)
                     /gene="agp"
                     /locus_tag="ACHKIK_002885"
     CDS             complement(19546..20787)
                     /gene="agp"
                     /locus_tag="ACHKIK_002885"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO9084024.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(21079..>21405)
                     /locus_tag="ACHKIK_002886"
     CDS             complement(21079..>21405)
                     /locus_tag="ACHKIK_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084025.1"
                     /translation="LPEFIFNDNKARLVLDTDPEQKVNEVKNFLTALSDSICSLFIVN
                     GNVASISLGNESFSTDDDLEYGYLINTGNHYDVYLPPELFAQAYELNNKERNAQIDFL
                     TRYAIY"
BASE COUNT         5054 a         5749 c         5534 g         5068 t
ORIGIN      
        1 ataatattat ccctaaagaa taaatcagag gaatatattc ggtgttaaac agtcagtcaa
       61 agtatagccc attgttgatt aaacctcata agcaaaattg gcaccagatc tcctgtacag
      121 gttgaatggc cgaaaaaaga taggcagatg ctcatttttt tggcttctgg aagaaaaact
      181 ctccgcgctg gcagtattac actggtctga tgctatgacg aaaaatgcct ggtaagagta
      241 tctaaaccag actccagttc agcttatcaa cacgctcaaa gtcgcccccc tgaacgctaa
      301 cctgaagtga aaatctgttg aaataaaaca aagcccggtt cgcccgggct ctgcaccgat
      361 aatacactta actgtaggca tgcagcgtac gttggcaaag tgccgaacgt acgcagtcct
      421 ctttaccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc agcgcgtcac
      481 ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg ttgacgataa
      541 ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca gtcacattct
      601 gcgcctcgtc aagaatgacg actgcatttt caaaggtacg tccacgcata taggcgaacg
      661 gcgcaatttc caccttccct atttccggtc gcaggcagta ctgcataaag gaagccccta
      721 agcgccggac cagcacgtcg tagaccgggc gaaaataggg agcaaacttt tctgcgatat
      781 ctccaggtaa gaagccaaga tcttcatcag cttgcagaac tggacgggtg acgataatcc
      841 tgtcgacatc cttatgtatc agggcttctg ccgcttttgc tgcgctgatc caggtttttc
      901 cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc ttcagatagt
      961 gcaattgcgc ttcatttcgc gcgaggatgg gcgaagtatc gcgactgtcg cgggccatac
     1021 caatggcttc tacgccgccc atctgcacaa gcgaggtgac cgattcttct tcacgctgct
     1081 tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct ttgatcactg
     1141 ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac gcgccgttgg
     1201 cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca tagtttgctt
     1261 ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta acacgagtaa
     1321 agagaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga aatctccgca
     1381 agtggtggag cgtagcttaa caagacattg tccagaacag gacgttacca tccgcgatct
     1441 ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataat tacaacagat
     1501 ctcgcattca ttgcaacaat atattcactt atatttaacc ataaaacacc atttcagtga
     1561 cattagtttc tactggaaag atgacagagt gatgacagat atgaaaaaag ctgtgtgctt
     1621 tcagcaggat tcgcatcagg catctgcgca cgaacgccgg atgcggcgtg aacgccttat
     1681 ccgcctaccg aacccgcacc gattgtaggc ataagaagac gcgcaagcat cgcatcaggc
     1741 atctgcgcaa gaacgcctta tccgtctata aacattaaac ccgcaataac gcgcttccga
     1801 gataatcgtt cgcgtccttc actcccggca gcgcaaaaaa ataaccgccg ccgataggtt
     1861 taacgtattc ctccagcgct tcgccattga gccttttttg tactgtcagg aagccttttt
     1921 ccagatcgtg ttggtaacag acaaacagta gccccatatc cagttgcccg gagttggtga
     1981 cgcccagtga ataactgtag ccacgacgca gcatcaggct ggactcactc tccgccgtgc
     2041 ggggattcgc cagccggata tggctgtcca gcgcaatcac cttcccttcc ggatcgctgg
     2101 cgtaatcggg aacatcatgc tcatgctgca ttcccagcgg cgcaccggtt tgcttatcac
     2161 gaccaaaaat cgtctgctgt tctttcagcg gagttctgtc ccaaaattcc actcgaaact
     2221 gaatcaagcg tactgcctga tagctgccac cgattgtcca cgctggctcc tgctgatctg
     2281 ccgttaccca caccactttt tgcatcaact tatcattctg gctatcggga ttggcagtgc
     2341 cgtctttgaa acccagcaaa ttaatcggcg tctctttgcc tttactacgc gccgcgtgat
     2401 cggaaataaa cccttcccgc ttccagcgca cgctgagcaa atccggcgta tgtttgatga
     2461 tatcgcgcag tgcatggatc accgtgtcct gggtgttggc gcaaatttgt agcaacacat
     2521 caccatgaca taacgccgca tccagcgagt cgttgggaaa acgcgtcatc ttctgcaact
     2581 ttttcggcat ctgtggcgca aggccaaagc gctcatcaaa caatgagtga cccaccgata
     2641 acgtgatggt gagattatcg ggcgcaatgt agccgccaag aatgccggaa tcgagtggtg
     2701 gcaggcgcgg atttggcgtt tctggtgccg ctccgccctg agtcagaaaa gcaaaacgct
     2761 gagtcaacaa gcgaaacaac cgttcaagat cggctttatc gctggcaagc acatcaaacg
     2821 ccaccagcat cattgcggcc tgttgtggcg tcaggatccc tgcctgatgc tcaccataaa
     2881 acggctgttt ctcattgcgt gcatccggtg aaagcgtacc cggcgcactt tgcgtttttt
     2941 gtgcatgagc gaccggacaa cttcccgcca gcgccagcgc accaatgcct ttcagtaaac
     3001 gtcggcgtga cggttcattc acgccgtttt tatcttcata ctgcataatg cttaatccaa
     3061 tcccagcaca ccgcgaagtt gcgccagatc ttccgccagc gcagtaatcg gtcctttcag
     3121 tgcattccgg tcggcatcgg tcaatttgtc gtaggtttca aaaccgtctt tagtacggta
     3181 tttcgccaga atggtatcga cctttttaaa gttggcatcg acttttgcca acagttccgg
     3241 gttggctttt tgcagttgtg gacgcagcag atcgacaatt ttctgcgagc cttcaacgtt
     3301 agcctggaaa tcccacagat cggtgtggct gtagcgatct tcttcaccgc tgattttgct
     3361 ggctgccact tcctcaatca gtccggctgc accgccgacc acttttgaag gtgggaaagc
     3421 cagttcactg atgcgttttt gcaaatcgac cacatcggta taaagctggt cagcgtactg
     3481 atccatgcct ttggtggtgt tgtcgccaaa caatgctttt tccagacggt ggaaaccggt
     3541 gaattttgga tcagcggctt tttgctcgta atcatcttca cgggcgtcaa tgctgccatc
     3601 cagatcggag aacagttcag caatcggttc aatgcgctca tagtgctggc gcgtcggcgc
     3661 atacagtgct ttcgcttttt cgatatcgcc tgctttaatc gcgtcggtaa aggctttggt
     3721 gtcggtcacc agctgcgtgg tttccgccat gacatacgct ttatatgcag taattgcacc
     3781 accaagactt aacagcgcat cactttgcgc cgcatccgcc gttgcctcac ctttgacgat
     3841 caacttccct ttcgggttag tcagcagacc gcaggtcata tcgtattcgc caggctgtaa
     3901 attcgccgtc attttctggc taaaaccagg ggcgatattt tcccgctctt ccaccaccat
     3961 cacgcctttg aggatctccc actccagcgc cttctggcta tggttctgaa taatgaactg
     4021 tgttttcccg gcgttaaccg taatggtcat cggttcgcac tgcttatccg tcacggtcac
     4081 tttgacctgc gtcacatcag cggcgttagc cataaaagca gaagaaaaca gcgcagccac
     4141 gctcaactgc aatgcgttac ggcggaagtt aatggtcatg acgagactat ccctttaaag
     4201 tatgttgtaa ctaagcaaga gtttgcgtcg tatttgttac gcggagcgag acgctgtcgc
     4261 ccctgcgcgt ggtggcagag caaatgccac cagcgccggg atgagataaa taaaccagac
     4321 ggcgacttcg ctgacgctcg gcgcttcctg atagccaaaa atcccttcca tcagcgtgcc
     4381 aaacagcgag tgagttgaga gcaccgcact catatcgaag gcgatttcct gaaagtggtt
     4441 ccacaatccg gcttcatgaa atgcgcgaat ggcaccagct gccagccctg cggcgacgaa
     4501 gagaataaac aggctggtcc atttaaaaaa tgcaccaaga ttgaggcgaa taccgcccca
     4561 gtagagcaag aagcctagca ccacggcagt agcaagaccg agcattgcac ccagcggcgg
     4621 ccagatcccg acatcttgtt gaaatgccgc cagcaggaaa aagaccgact ccagcccttc
     4681 ccttgcaacg gcaaaaaaga ccatcatcac cagcgcccag ccatgatgat ttccacgctg
     4741 caatgcgcta tcgactgcct gctccagttg tactttgacg ttgcgcgaca ctttgcgcat
     4801 ccagaaaacc atccaggtaa ggatcaccac ggcgatcacc gccacgatac cttcaaacag
     4861 ttcctgctct ttttgcggaa attcgccggt ggtttcgtta atgaagatcc ccaagcccag
     4921 gcacaacgca gcggcaagca acacgccaat ccacatcaca ccaatccatc ggcctcgctg
     4981 ggtacgctta agatagctgg caatcaaact gacaatcagc gcggcttcaa gtccttcgcg
     5041 caacataatg agaaacggaa caaacatgcc acccatcctt aaacgttttt ctgagtcaat
     5101 ctatgcaaag aaaagtaaat cgcagtgata gcgattatca ttacggtaaa gaaaaataca
     5161 agcggaatgt ttataaaatg atgttgaaat gtaaacaaag agaggtaatg ccaggggata
     5221 taaacgatta ttccaggtac aaagatggga ttgtttacaa cgagccatgt gttcttgccg
     5281 gatgccgcgt aaacgccgta tccagccgac aaaaccgtcc tgactcaata catcaaaact
     5341 tccatgtaaa actgataagc gggtgtatca agcaatttcg tccatcctcg attatcaaat
     5401 ctaacttttt gcccggaaca taagaacagc gcgggtgttg gtggcgtcaa aatcgctgaa
     5461 acgttccccg aaggccgggg cagcccacat ggatgtgggc tgagggcgcg tcttacagga
     5521 cgttacctcg cgcccgaccc ggtagccgaa ggggataagc tgaaggtacc acgcagcggc
     5581 gattttgttc gccagagccc gggggtgcag ggggccgcgg cgattggccg ccccctgcgc
     5641 gctccctgca ccattcaatt tatatggcga ggcacattaa gggagcgcaa cccgctgctg
     5701 aagccaaaca aaaccgccgc aggctaagtc ccttagcttt cctgcaaccg tgacggcgga
     5761 gccgaatgat agtgcgcatc ggcctcggca aagcgttttt gcatcgccgc tgacggcgct
     5821 ttacccagca aactaaacac tacaatcccg atactgccga agatgaagcc cggaataatt
     5881 tcgtagagtc ccagccagcc gaactgcttc cagacgataa ccgtcagcgc accgatgatc
     5941 atccccgcca gcgcaccgtt gcgcgtcatg cgtgaccaca tcaccgagaa cagcaccacc
     6001 ggaccaaacg ccgcgccaaa gcctgcccac gcgtagctca ctaagcccag cacgcggttt
     6061 tccgggtttg ccgccagcgc aatcgccacc agcgccacca ccagcaccat cacacgtcct
     6121 acccacacca gctctttctg gctggcctgt ttacgcagaa acgctttgta taaatcttcg
     6181 gtaattgcac tggaacatac cagcagctgg caacttaagg ttgacattac cgccgccaga
     6241 atcgccgaca gcagaatccc ggcaatccac gggttaaaca gaatttgcgc cagttcgata
     6301 aacacgcgct cggcgttctg gtttaccgcg cctgctaccg ccgggtgctc attaaagtaa
     6361 gcaatcccaa agaagccaac agccaccgcc cctgccaggc agaggatcat ccaggtcata
     6421 ctgatacgac gcgcatggac aatgctgtgg tgagagtctg ccgccataaa acgcgccagg
     6481 atgtgcggct gcccgaagta acccagcccc caacccatca gtgagataat ggcgacaaag
     6541 ttcagccctt tgagcatatc aacgttttcg atgctctttt gtttgatcac ttccagcgag
     6601 tcaccaaagc caccgacact gataatgacg ataaccggcg tcaggatcag ggcaaaaatc
     6661 atcaggctgg cctgtacagt gtcagtccag ctcaccgcga ggaaaccgcc aataaaggtg
     6721 taaaggatcg tcgccgcagc tcccgcccac agagccgttt cgtagctcat gccaaaggta
     6781 ctttcaaaca gacgcgcgcc tgccacaatg cccgaagcgc aataaatggt gaagaacagc
     6841 aaaatcacca gcgcggagat aatgcgcaaa atgcggcttt tatcttcaaa gcgcccggtg
     6901 aaataatccg gcaatgttaa ggcgttattg ttgtattcgg tatgcacacg caaccgcccc
     6961 gccaccagct tccagttgat ccacgcgcct aatgtcaggc caatggcgat ccaactttcg
     7021 gaaatcccgg aaagaaaaac cgcgcccggc aaccccatta acagccagcc gctcatatcc
     7081 gacgcacccg ccgataatgc cgtcacgaat ggcccaaggc tacgaccgcc cagaatatag
     7141 tcgtcaaagt ttttcgttga tcgccaggcg ataaacccaa tcaatatcat gccaaagata
     7201 tagacacaaa atgtcaccaa catcggtgtg ctaatagcca tctaaagtct ccaaaaaatt
     7261 attatcggca atgtcgaaac ttgccgttat atctgccacc ggaacggggt aacagagttt
     7321 atgttttacc agggcgaccg tatcctgccg gaagcgctgg ttattcacaa tcgatttaac
     7381 acaccattta cattaaattt tagtgctcag cgacactatt tttcatcagg ttgcactctc
     7441 tcacattttt tgcggttgca cctttcaaaa atgttaactg ccgcagagaa aaagtctgag
     7501 ttattttttt aatccctgtc atatcgattt cttttattaa catttcattc atttttaagc
     7561 ttgctacgca tgtcacattt aacatggttg cacaaagttg caacatcatg gatatttcac
     7621 gataacgtta agttgcacct ttcagaacaa caggagtaat ggcatgggaa ccaccaccat
     7681 gggggttaag ctggacgacg cgacgcgtga gcgtattaag tctgccgcga cacgtatcga
     7741 tcgcacgcca cactggttaa ttaagcaggc gattttttct tatctcgaac aactggaaaa
     7801 cagcgatact ctgccggagc tacctgcgct gctttctggc gctgccaatg agagcgatga
     7861 agcaccgact ccggcagagg aaccacacca accattcctc gactttgccg agcaaatttt
     7921 gcccctgtcg gtttcccgcg ccgcgatcac agcggcctat cgccgcccgg aaaccgaagc
     7981 ggtttctatg ctgctggaac aagcccgcct gccgcagcca gttgctgaac aggcgcacaa
     8041 actggcgtat caactggccg ataaactgcg taatcaaaaa aacgccagtg gtcgcgcagg
     8101 tatggttcag gggttattgc aggagttttc gttgtcatcg caggaaggcg tggcgctgat
     8161 gtgtctggcg gaagcgttgt tgcgtattcc cgacaaagcc acccgtgatg cgttaattcg
     8221 cgacaaaatc agcaacggta actggcaatc acacattggt cgtagcccgt ctttgtttgt
     8281 taatgccgcc acctgggggc tgctgtttac tggcaaactg gtttccaccc ataacgaagc
     8341 cagcctctcc cgctcgctga accgcattat cggtaaaagc ggtgaaccgc tgatccgcaa
     8401 aggtgtggat atggcgatgc gcctgatggg cgagcagttc gtcactggcg aaaccatcgc
     8461 ggaagcgtta gccaatgccc gcaagctgga agagaaaggt ttccgttact cttacgatat
     8521 gcttggcgaa gctgcactga ctgccgcaga tgcacaagcg tatatggttt cctatcagca
     8581 ggcgattcac gccattggta aagcgtccaa tggtcgtggc atctatgaag gtccgggcat
     8641 ttcaatcaag ctgtcggcgc tgcatccgcg ttacagccgc gcccaatatg accgggtaat
     8701 ggaagagctt tacccacgtc tgaaatcact caccctgctg gcgcgtcagt acgatattgg
     8761 tatcaacatt gacgccgaag aggccgatcg tctggagatc tccctcgatc tgctggaaaa
     8821 actctgtttc gagcctgagc tggcaggctg gaacggtatc ggttttgtta ttcaggctta
     8881 tcaaaaacgc tgcccactgg tgattgatta cctgattgat ctcgccaccc gcagccgtcg
     8941 ccgtctgatg attcgcctgg tgaaaggcgc gtactgggat agtgaaatca agcgtgcgca
     9001 gatggacggc cttgaaggct atccggttta tacccgcaag gtgtataccg acgtttcata
     9061 tctcgcctgt gcgaaaaagc tgctggcggt gccgaatcta atctacccgc agttcgcgac
     9121 gcacaacgcc catacgctgg cagcgattta tcaactggca ggtcagaact actatccggg
     9181 tcagtacgag ttccagtgcc tgcatggtat gggcgagcca ctgtatgagc aggtcaccgg
     9241 gaaagttgcc gacggcaaac ttaaccgtcc gtgtcgtatt tatgctccgg tcggtaccca
     9301 tgaaacgctg ttggcgtatc tggtgcgtcg cctgctggaa aacggtgcta acacctcgtt
     9361 tgttaaccgt attgccgaca cctctttgcc actggatgaa ctggtcgccg atccggtcac
     9421 tgctgtagaa aaactggcgc aacaggaagg gcaaactgga ttaccgcatc cgaaaattcc
     9481 cctgccgcgc gatctttacg gtcacgggcg cgacaactcg gcagggctgg atctcgctaa
     9541 cgaacaccgg ctggcctcgc tctcttctgc cctgctcaat agtgcactgc aaaaatggca
     9601 ggccttgcca atgctggaac aaccggtagc ggcaggtgag atgtcgcccg ttattaaccc
     9661 tgcggaaccg aaagatattg tgggctttgt gcgtgaagcc acgccgcgtg aggtagaaca
     9721 ggcgctggaa agtgcggtta ataacgcgcc aatctggttt gccactcctc cggttgaacg
     9781 cgcggcgatt ttgcatcgcg ctgccgtgct aatggaaagc cagatgcagc aactgattgg
     9841 tattctggtg cgtgaagccg gaaaaacctt cagtaacgcc attgccgaag tgcgcgaagc
     9901 ggtcgatttt ctccactact atgccggaca ggtgcgggat gatttcgcta acgaaaccca
     9961 ccgtccatta gggcctgtgg tgtgtatcag tccgtggaac ttcccgctgg ctattttcac
    10021 cgggcagatt gctgccgcac tggcggcagg taacagcgtg ctggcaaaac cggcagaaca
    10081 aacgccgctg attgccgcgc aagggatcgc cattttgctg gaagcgggtg taccgccagg
    10141 cgtggtgcag ttgctgccag gtcaggggga aaccgtgggc gcgcaactga cgggtgatga
    10201 tcgcgtacgc ggggtgatgt ttaccggttc aaccgaagtc gctacgttac tgcagcgcaa
    10261 tatcgccagc cgcctggacg ctcagggtcg ccctattccg ctcatcgctg aaaccggcgg
    10321 catgaacgcg atgattgtcg attcttcagc actgaccgaa caggtcgtcg tggatgtact
    10381 ggcctcggcg ttcgacagtg cgggtcagcg ttgttcggcg ctgcgcgtgc tgtgcctgca
    10441 agatgagatt gccgaccaca cgttgaaaat gctgcgcggc gcaatggccg aatgccggat
    10501 gggtaatccg ggtcgcctga ccaccgatat cggtccggtg attgatagcg aagcgaaagc
    10561 caatattgag cgccatattc agaccatgcg tagcaaaggc cgtccggtgt tccaggcggt
    10621 gcgggaaaac agcgaagatg cccgtgaatg gcaaagcggc acctttgtcg ccccgacgct
    10681 gatcgaactg gatgactttg ccgaattgca aaaagaggtc tttggtccgg tgctgcatgt
    10741 ggtgcgttac aaccgtaacc agctaccaga gctgatcgag cagattaacg cttccggcta
    10801 tggtctgacg cttggtgtcc atacgcgtat tgatgaaacc atcgcccagg tcactggctc
    10861 ggcccatgtt ggtaacctgt atgttaaccg taatatggtg ggcgcagtgg tgggtgtgca
    10921 gccattcggc ggcgaagggt tgtccggtac cgggccgaaa gcaggcggtc cgctctatct
    10981 ctaccgtctg ctggcgaatc gcccggaaag tgcgctggca gtgacgctcg cgcgtcagga
    11041 tgcagagtat ccggtcgatg cacagttgaa agccgcattg actcagccgc taaatgcact
    11101 gcgggaatgg gcagcaaatc gtccagaatt gcaggcgtta tgtacgcaat atggcgagct
    11161 ggcgcaggca ggaacacaac gattgctgcc ggggccgacg ggtgaacgca acacctggac
    11221 gctgctgccg cgtgagcgcg tgttgtgtat tgccgatgat gagcaggatg cgctgactca
    11281 gctcgccgcc gtgctggcgg tgggcagcca ggtactgtgg ccggatgacg cgctgcatcg
    11341 tcagctagta aaggcattgc catcggcagt cagcgaacgt attcaactgg cgaaagcgga
    11401 aaatataacc gctcaaccct ttgatgcggt gatcttccac ggtgattcgg atcagcttcg
    11461 cgcattgtgt gaagccgttg ctgcgcggga tggcgcaatt gtttcggtgc agggttttgc
    11521 ccgtggcgaa agcaatatcc ttctggaacg gctgtatatc gaacgttcgc tgagtgtgaa
    11581 taccgctgcc gctggcggta acgccagttt aatgactata ggttaaacag gtagccggag
    11641 gatgttacaa cctcctccgg catctttaac gtggtcgaat cccctcaata ataatccgct
    11701 gcacgttttc aaccgtttga ttgaaaaata tctcatcgcg caacgtcgcg cctgtcaccg
    11761 cctccacctg aggggcgaaa tcggcgtaat gttgagtgga agcccaaatc atgaaaatca
    11821 aatgctgcgg atcaatcggc gcgagtttgc cgcttttgac ccaaccggca atcagcgccg
    11881 atttctcatc aattaatgcc ttcaaatcgc ccgtcagttc atccattaac agcggcgcgc
    11941 ctgccagcat ctccatacag aacaggcgcg aagcctgcgg ataatcgcgg gagacttcca
    12001 gcttcagacg gatgtactct tttatcgccg ccagcggagc gaaatcttca cgaaacgctt
    12061 ttaacggtgc cagccagata tcgagaatct gccgcagcac ggcaatatac agcgcctctt
    12121 ttgacggaaa gtaatacagc agattggttt ttgaaacacc cgccaactct gcgatctgct
    12181 ccagccttgt gccgtgaaaa ccgaattgtg aaaaagtgtc cagtgctgcg ctaagaatcg
    12241 cttttttctt cgcgcttact gcgcgcgaac gtttacccgt tgttttcact gcgccttgcg
    12301 tcatgcgctc tcccctcttg tagattggca acaggatagc aaaacccccg cccggcctcg
    12361 accttctgca ctctcatcgc gcacagatgc atgttttatg tgcaactgtt ttgaccgttt
    12421 agtccacttt ttaccagata ttttagacaa cacaatgtta acacattaat aacattcact
    12481 ttttaaaaac tggcatccgc tttgcaaaca agccattacg cggctgaaaa ggatatgcag
    12541 gatgcagcgc cccgtctgac tttcacttta cgagacgaag agaggttgat gatgaaaatt
    12601 ggcgtattcg tacctattgg caacaacggc tggctcattt cgacccacgc gccgcagtac
    12661 atgccgacct ttgaactgaa taaagccatt gtgcaaaaag cggagcacta ccatttcgat
    12721 ttcgccctgt cgatgatcaa actgcgtggc tttggcggca aaactgagtt ctgggatcac
    12781 aaccttgagt cgttcacctt gatggcgggg ctggcggccg tcacctcgcg cattcagatt
    12841 tacgccactg ctgccacctt aacgttacct ccggcaatcg tcgcccgtat ggccgcaacc
    12901 atcgactcca tctctggcgg gcgttttggc gtcaacctcg tgactggctg gcaaaagccc
    12961 gagtatgagc agatgggtat ctggcctggc gatgactatt tctcccgtcg ttacgactat
    13021 ctcaccgaat atgttcaggt gctgcgcgac ctgtggggca cggggaaaag cgattttaaa
    13081 ggcgattttt tcaccatgaa tgattgtcgc gtcagtccgc aaccgagtgt ccccatgaaa
    13141 gtgatctgcg ccgggcaaag cgacgctggc atggcgttct ccgcccagta tgccgatttc
    13201 aacttctgtt tcggcaaagg cgtaaatacg cccacggctt tcgccccgac cgctgcgcgg
    13261 atgaaacagg ccgcagagca aaccgggcgc gacgttggtt cttatgtgtt gtttatggtg
    13321 attgccgatg aaaccgacga tgccgctcgc gccaaatggg aacactacaa agcgggcgcg
    13381 gatgaagagg cgttaagctg gctaaccgaa caaagtcaga aagatacccg ctccggtact
    13441 gataccaacg tccgtcagat ggccgatccc acttcggcgg taaacatcaa tatggggacg
    13501 ttagtcggtt cttacgccag tgtcgcgcgc atgttagatg aagtcgcaag cgtgcctggt
    13561 gccgaaggcg tgctgttgac cttcgatgat tttctgtcgg gaatcgaaac cttcggcgag
    13621 cgcattcaac cactgatgca gtgccgcgcc catctacctg cgctgactca ggaggtggca
    13681 tgatgacaac cttaaccgct cgaccggaag ccattacctt cgatccgcag caaagtgcgc
    13741 tgatcgtggt ggatatgcaa aacgcttatg ccacgccagg cggctactta gatctcgccg
    13801 ggtttgatgt ctcaaccact cgcccggtca ttgccaacat tcaaaccgcc gtgaccgcag
    13861 cgcgagcggc agggatgctg atcatctggt ttcaaaatgg ctgggatgaa cagtatgtcg
    13921 aagctggcgg ccccggctca ccgaattttc ataaatcgaa cgccctgaaa accatgcgta
    13981 agcagccgca gctgcagggg aaattgctgg cgaaaggctc ctgggattat caactggtgg
    14041 atgaactggt gccgcagcct ggcgatattg tgctgccgaa gccgcgctac agcggtttct
    14101 tcaatacacc gctggacagc attttgcgca gccgtggaat acgccatctg gttttcaccg
    14161 gtatcgctac caacgtctgc gtcgaatcga cgctacgcga tggctttttt ctggagtatt
    14221 tcggcgtggt gctggaagac gcaacacacc aggcggggcc ggaatttgca cagaaagccg
    14281 cgttgttcaa tatcgaaacc ttttttggct gggtcagcga cgtcgaaacg ttctgcgacg
    14341 cgctttctcc cacatccttt gctcgtatcg cttaaggagt ttaacgatgc caaaatccgt
    14401 aattattccc gctggcagca gcgcaccgct ggcccccttc gttcccggca cgctggctga
    14461 tggcgtggtg tatgtctccg gtacgctggc ttttgatcaa cataataacg tgctgtttgc
    14521 cgatgaccca aaggcgcaaa cccgccacgt tctggaaatt atccgcaagg tgatcgagac
    14581 ggcgggtggc acgatggcgg atgtgacctt caacagcatc tttattaccg actggaaaaa
    14641 ttacgccgcg attaacgaaa tctacgccga gttttttccg ggtgataaac cggcgcgatt
    14701 ctgcattcag tgcggactgg taaaacctga cgcgctggtg gaaatcgcca caattgcgca
    14761 tatcgccaag tgaggccgcg atgaaacttt cactctcacc tcccccttat gctgatacgc
    14821 ccgtagtggt gttgatttcg ggtcttggcg gtagcggcag ttactggtta ccgcaactgg
    14881 cggtgctgga gcaggagtat caggtagtct gttacgacca gcgcggcacc ggcaataatc
    14941 ccgacacgct ggcagaagat tacagtatcg cccagatggc agcggaactg catcaggcgc
    15001 tggtagccgc agggattgag cattacgcgg tggtcggcca tgcgctcggt gcgctggtgg
    15061 gaatgcagct ggcgctggat tatcccgcgt cggtaactgt gctgatcagc gttaacggct
    15121 ggctacgaat aaacgcccat acgcgccgct gttttcaggt tcgcgaacga ttactgtata
    15181 gcggcggcgc gcaggcatgg gtggaagcgc agccgttgtt cctctatccc gccgactgga
    15241 tggcggcccg cgcacctcgc ctggaggcag aagacgcgct ggcactggcg cattttcagg
    15301 gcaaaaataa tttactgcgt cgacttaacg ccctcaaacg cgctgacttt agtcaccatg
    15361 cggatcgcat ccgctgcccg gtgcaaatca tctgcgccag tgatgatctg ctggtgccat
    15421 cagcatgttc cagtgagctt catgccgccc tgcccgatag ccagaaaatg gtgatgccct
    15481 atggcggaca cgcctgcaac gtgaccgatc ccgaaacgtt taatgctctg ttactcaacg
    15541 ggcttgccag cctgttacat caccgtgaag ccgccctgta aggaattgct atgaacgaag
    15601 ccgttagccc aggtgcgctt agcaccttgt tcaccgatgc ccgcactcac aacggctggc
    15661 gggagacacc cgtcagcgat gagacgttac gggagattta tgccctgatg aaatgggggc
    15721 cgacatcagc taactgttct ccggcacgga tcgtgtttat ccgcacggca gaaggaaaag
    15781 aacgtctgcg cccggcactt tccagcggca atctgcaaaa aaccctgacc gcgcccgtca
    15841 ccgctatcgt cgcctgggac agtgaatttt atgaacggtt accgcaactg tttcctcacg
    15901 gcgatgcccg cagttggttt acctccagcc cacaacttgc cgaagaaaca gcgtttcgca
    15961 acagttccat gcaggcggcc tatctgatcg tcgcctgccg tgcgctggga ctggataccg
    16021 gcccgatgtc gggttttgac cgtcaacacg ttgacgacgc cttttttgcg ggcagcacgc
    16081 tgaagagcaa tctgctgatt aatatcggct atggcgatag cagcaaactt tttgcgcgcc
    16141 tgccacgtct gtcctttgaa gaagcctgcg ggctgttgta aggagcaaac catgaacatt
    16201 gtcgatcaac aaacctttcg cgatgcgatg tcctgtatgg gcgcggcagt caatatcatc
    16261 accacggatg gtccagccgg gcgcgccggg ttcaccgcca gcgcggtctg cagcgtgacc
    16321 gacacgccgc ctacattact ggtgtgcctg aatcgtgggg cgtccgtctg gccggtattc
    16381 aatgaaaatc gaacgctgtg tgtaaataca ctgagcgccg ggcaggagcc gctttcaaat
    16441 ctttttggcg gcaaaacgcc catggaacac cgctttgccg ccgcccgctg gcagacgggt
    16501 gtgaccggat gcccgcaact ggaagaggct ctggtttcgt ttgactgccg tatcagccag
    16561 gtggtgagcg tcggcaccca cgacattctg ttttgcgcca tcgaagcgat tcatcgtcac
    16621 gccacaccct acgggctggt gtggttcgat cgcagttatc acgcgctgat gcgccctgct
    16681 tgttaacctc ctaaaggaga cagctgatgg caatgttcgg ttttcctcac tggcagttaa
    16741 aatcgacctc tacagagagc ggcgtggtcg cgccgtatga acgattgccg tttgctcaga
    16801 cggcagtaat gggcgttcaa cacgcggtgg cgatgtttgg cgcgacggtg ttaatgccga
    16861 ttctgatggg gctggatccc aatctttcca ttttaatgtc ggggatcggc acgttactgt
    16921 ttttctttat taccggcggg cgcgtcccca gttatctcgg ttccagcgcg gcttttgtcg
    16981 gcgtggtgat tgccgcgacg ggctttaacg gtcagggcat taacccgaat atcagcattg
    17041 cgctgggcgg gattatcgcc tgtgggctgg tttataccgt cattggcctg gtggtgatga
    17101 aaattggtac acgctggatt gaacgactaa tgccgccggt ggtgacgggt gcggtggtga
    17161 tggcgattgg cctgaactta gcgcctatcg cagtgaaaag tgtatcggct tcggcctttg
    17221 atagctggat ggcggtaatg acggtgctgt gtattgggct ggtggcggtg tttacccgtg
    17281 gcatgatcca gcggttattg attctcgtag gtttaattgt cgcctgtctg ctgtatggcg
    17341 tgatgaccaa ccttttaggg ctgggaaaag ccgtcgattt tactctcgtc agccatgctg
    17401 cctggttcgg tttgccacat ttttccactc ctgcttttaa tagtcaggca atgatgctga
    17461 ttgcgcccgt ggcggtgatt ctggtggcgg aaaacttagg tcatctcaag gcggtcgccg
    17521 ggatgaccgg acgcaatatg gacccgtata tggggcgggc attcgtcggt gatgggctgg
    17581 cgacgatgct ttctggttct gtcggtggca gcggcgtaac cacctacgcc gagaatatcg
    17641 gtgtaatggc cgtgacaaaa gtctactcga cgctggtgtt tgtcgctgct gccgtcatcg
    17701 ccatgctgtt gggcttttca ccgaaatttg gcgcgctgat tcataccatt ccggccgcgg
    17761 tgattggtgg tgcatcaatt gttgtgttcg ggctgattgc cgtcgcaggc gcaaggatat
    17821 gggtacaaaa ccgtgtcgat ttaagccaga acggtaattt gattatggtc gcagtaacct
    17881 tagtgctggg cgcgggtgat tttgctctca cgctgggcgg ttttacgttg ggagggattg
    17941 gtacagcaac ctttggcgcg attttactca atgcgttgtt aagcagaaag ttggttgacg
    18001 ttccaccgcc agaagttgtt catcaggagc cataaacaga aaaccgccgg gattaccggc
    18061 ggtgatgact ttttgacgag cctaagaaaa aaatgcattc gtcattgatt agcccggcta
    18121 gttgtcgctt ttgccgtgac tgctcttgcc acctttttta cctgcttcag atgcgcgctg
    18181 cgggtcattc ttgaaattac ccccgctgtg ctgtccacct tttttacctg cttctgatgc
    18241 tctttcgcgg tcttctgcaa aattgccgga accgcctcga tggtttgcca ttactgacct
    18301 ccgtctgggt atagacatca tattgcatag aattaacgcg gcatatggcg gctgccatat
    18361 tttcgctacg tgggactaag cttagtgaac tgacgcgggc agacccgcgt ttggtaatat
    18421 cctgcaacag cttcttagcg agaatagata tcatctcaaa taattatcca taagccgctc
    18481 ttatgattaa aaaaacgcga tgagataaaa atgtgtcatt ttgcgacaaa attacgtgct
    18541 tgtgaaagtt gttataaatc aaataagtgg ttgtgaaatt tgcactctga aaaggacgtc
    18601 ttatctttaa ataagtggta gcgaatcgct acggaataga gataacacga ggagtggtta
    18661 gaaatggcta aagttctggt gctttattat tccatgtacg gacatattga aacgatggca
    18721 cgcgcagtcg ctgagggtgc aagcaaagtc gatggcgcag aagttgtcgt taagcgtgta
    18781 ccggaaacca tgccgccgca attatttgaa aaagcaggcg gtaaaacgca aactgcaccg
    18841 gttgcaaccc cgcaagaact ggccgattac gacgccatta tttttggtac acctacccgc
    18901 tttggcaaca tgtccggtca aatgcgtacc ttcctcgacc agacgggcgg cctgtgggct
    18961 tccggcgcac tatacggaaa actggcgagc gtctttagtt ccaccggtac tggcggcggt
    19021 caggaacaaa ctattacttc aacctggacg acccttgcgc atcacggcat ggtaattgtc
    19081 cccattggct acgcagcgca ggaattattt gacgtttcac aggttcgcgg cggtacgccg
    19141 tacggcgcaa ccaccatcgc aggcggtgac ggctcacgcc agccaagcca ggaagaactg
    19201 tctattgctc gttatcaagg ggaatatgtc gcaggtctgg cagttaaact taacggctaa
    19261 tcttcaacag gaggatacgc atgccaactc aagaagcgaa agctcatcac gtcggtgaat
    19321 gggcatctct gcgcaatacg tcgccggaaa tagccgaggc cattttcgaa gtcgccgggt
    19381 acgacgaaaa aatggcggaa aagatttggg aagaaggtag cgatgaagtg ttagtcaaag
    19441 cctttgctaa gaccgataaa gattcgcttt tttggggcga acagaccatc gaacgtaaaa
    19501 acgtttaacc agcgactccc ccacttctcg cgggggagtt ttctgttatt tcaccgcctc
    19561 attcaacacg ctatcaaact tatccattgg gcagaaaccg ttggcatcta tcgggcaacc
    19621 gcttaattcc agcgtcacac gctgagcagg tgcctgcagg gttaacgcat cggcattacg
    19681 taactgttcc gcactctgat acacatattc aattttcatc aaatcgcgat tggctttgct
    19741 gtcatgccaa cgctggaaaa cgattttgcc gccaatcggc gtgcgttcgt tctggtcatg
    19801 caactgatac ggtttgaaat ccagcgccgt taacagagag gcaatgttgg agtcgtgccc
    19861 aaccaacact gtaattttcg gtgcgctggt gcgatcggtg accagagctt tgtcgatata
    19921 actgaccagc ggtttcgcaa cattgcgcgc cacttccggt gaggtaaaca ggctgtcctg
    19981 gtagccgttt ttaagcttcg acaacacctt ccactgctgg tcagatttga tttctcccca
    20041 ggccacctga tccatcggaa aaccttcgta atattgcaaa gtaaacgcat ctaccagcga
    20101 gttgccgact ttcagcggcc cggaaacacc tggttcttgt tgatacttgg cgctaaaggt
    20161 atttttgcca tccaccagcg aacactgttg tttctcttta caggcagggg aatctttata
    20221 gttaacgatt ttttccagta gttggtagct gtcggtaagc tggagtttgc tgagctcttt
    20281 ctccattgcc gccaccgcct gttcactgaa tgcggcggaa tcatcggtga tcaccgggtt
    20341 aaaggttggg tccatagtgc ccattttttc ctggtgatgc acaggaatat cacaccccgg
    20401 gaatgcgccg gtaataaaga actgtgcggt cgcaacggta cgttgcagac tattggcata
    20461 ggcataaacg gtgtccggcg gcgggcattc ccccgatttc accatcccct gctgtgccag
    20521 ccattcacgc atgtaatggc ccatatacac ttcgagcacg ccacctttgg tggtgagttg
    20581 cccaccgggg acgtcccatt cgggccattt attcggcgta gactgctcca gcacactgcc
    20641 attgttcgcc agcggcgcac gtaagttatg gcggctcatc atgagcactt gctgtagctg
    20701 atagccttcc ggtacggttt gtgcctgagc atttgaagcg agtaaaacta tccctgccac
    20761 agttgcggcg attagcgttt tgttcattcc tggcacctct tttgttatca atcactaaag
    20821 agtgtgacag aaatatgaca tttttccggg aacaggttcg caaaatggct tgttgaatgc
    20881 ctgtttttct atatacatct ttaaaaacag gcaaggtttg acgcaaacta tgcctgatta
    20941 gctccctgcc tcgcgtacgc cgctgatgat tacacctgtg gctaaacaga atatcagcgg
    21001 cgttactaat acagaggcaa tagtaccaat aataagagaa gtgaggctaa taacaatggt
    21061 ttttagcaca tgcctttgtc agtaaattgc ataacgagtt aaaaaatcaa tttgcgcatt
    21121 cctttcctta ttgtttaact cgtaagcctg agcaaaaagt tcaggaggga ggtaaacgtc
    21181 ataatgattg ccagtgttta ttaaataccc atactcaagg tcgtcatctg ttgaaaagga
    21241 ttcattccct aaggagattg atgccacatt accattaaca ataaataatg agcaaatgct
    21301 atctgaaaga gcagtgagga aatttttcac ctcattaacc ttctgctctg ggtcggtgtc
    21361 taaaacaagg cgtgccttat tatcattaaa tataaactcg ggtaa
//
LOCUS       DAZGJW010000076        21378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZGJW010000076 DAZGJW010000000
VERSION     DAZGJW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.076"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..288
                     /locus_tag="ACHKIK_002887"
     CDS             <1..288
                     /locus_tag="ACHKIK_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO9084026.1"
                     /translation="TPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMP
                     ASAAYRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            1361..1684
                     /locus_tag="ACHKIK_002888"
     CDS             1361..1684
                     /locus_tag="ACHKIK_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFO9084027.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            1668..2144
                     /locus_tag="ACHKIK_002889"
     CDS             1668..2144
                     /locus_tag="ACHKIK_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO9084028.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            2141..2578
                     /locus_tag="ACHKIK_002890"
     CDS             2141..2578
                     /locus_tag="ACHKIK_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO9084029.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            2784..3302
                     /locus_tag="ACHKIK_002891"
     CDS             2784..3302
                     /locus_tag="ACHKIK_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO9084030.1"
                     /translation="MNHQLANLDFRDMVVVSGDRVITTSRKVAAYFDKQHHHIIQKIE
                     KLDCSDEFLTSNFSRVTYEHKGNQYVEYEISKDGAMYIIMSFTGKKAAAIKEAFIKAF
                     NWMRDRLMEMAHSYQREHNELMLEFMKEKDVASMSGRLLNRWGRIKKPQLIARIKRLE
                     QQAQISIPGLPK"
     gene            3586..3957
                     /locus_tag="ACHKIK_002892"
     CDS             3586..3957
                     /locus_tag="ACHKIK_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HFO9084031.1"
                     /translation="MNDQQIEKEIVEKGKTAPRVTPDHIEGIIAQEAYFTAEDGAFGK
                     AIKAKHTGGEVNYQSHESLSLLTFCVLVLRNGFTVTGESACASQENFDAEIGRKIARQ
                     NAVNKIWMLEGYLLKQKLSEQ"
     gene            4061..4303
                     /locus_tag="ACHKIK_002893"
     CDS             4061..4303
                     /locus_tag="ACHKIK_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HFO9084032.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITTRTIEAVRKSKEALDLFTTGA"
     gene            4383..4808
                     /locus_tag="ACHKIK_002894"
     CDS             4383..4808
                     /locus_tag="ACHKIK_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin carboxyl-hydrolase"
                     /protein_id="HFO9084033.1"
                     /translation="MATEKKKGGRPSDYMPEVANDICALLSSGESLRKVCERPGMPSK
                     TSVFRWLAEHQEFRDQYAKATETRADSIFEEIFEIADDVIPDAAEVAKARLRVDTRKW
                     ALARMNPRKYGDKVTNELVGKDGGAIQIETSPMSTLFGK"
     gene            4805..6217
                     /locus_tag="ACHKIK_002895"
     CDS             4805..6217
                     /locus_tag="ACHKIK_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFO9084034.1"
                     /translation="MTSINPIFEPFIEAHRYKVAKGGRGSGKSWAIARLLVEAARRQP
                     VRILCARELQNSISDSVIRLLEDTIEREGYSAEFEIQRSMIRHLGTNAEFMFYGIKNN
                     PTKIKSLEGIDICWVEEAEAVTKESWDILIPTIRKPFSEIWVSFNPKNILDDTYQRFV
                     VNPPDDICLLTVNYTDNPHFPEVLRLEMEECKRRNPTLYRHIWLGEPVSASDMAIIKR
                     EWLEAATDAHTKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINE
                     GADWATSLAIEDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDGP
                     YQAGAWADEVVQGDNVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLS
                     FDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCM
                     HCPALVREETEIYVPSSSGW"
     gene            6220..8346
                     /locus_tag="ACHKIK_002896"
     CDS             6220..8346
                     /locus_tag="ACHKIK_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO9084035.1"
                     /translation="MAETLEKKHERIMLRFDRAYSPQQEVREKCIEATRFARVPGGQW
                     EGATAAGTKLDEQFEKYPKFEINKVATELNRIIAEYRNNRITVKFRPGDREASEELAN
                     KLNGLFRADYEETDGGEACDNAFDDAATGGFGCFRLTSMLVNEYDLMDDRQRIAIEPI
                     YDPSRSVWFDPDAKKYDKSDALWAFCMYSLSPEKYEAEYGKKPPASLDVTSMTSWEYD
                     WFDEDVIYIAKYYEVRKESVGVISYRHPITGEIATYDSDQVEDIEDELAIAGFQEVAR
                     RSVKRRRVYVSVVDGDGFLEKPRRIPGEHIPLIPVYGKRWFIDDIERVEGHIAKAMDP
                     QRLYNLQVSMLADTAAQDPGQIPIVGMEQIRGLEKHWEARNKKRPAFLPLREVRDKSG
                     NIIAGATPAGYTQPAVMNQALAALLQQTSADIQEVTGGSQAMQQMPSNIAQETVNNLM
                     NRADMASFIYLDNMAKSLKRAGEVWLSMAREVYGSEREVRIVNEDGSDDIAVLSAQVV
                     DRQTGAVVALNDLSVGRYDVTVDVGPSYTARRDATVSVLTNVLSSMLPTDPMRPAIQG
                     IILDNIDGEGLDDFKEYNRNQLLISGIAKPRNEKEQQIVQQAQMAAQSQPNPEMVLAQ
                     AQMVAAQAEAQKATNETAQTQIKAFTAQQDAMESQANTVYKLAQARNIDDKAVMEAIR
                     LLKDVAESQQQQFQSPPQSPADLMPS"
     gene            8360..9244
                     /locus_tag="ACHKIK_002897"
     CDS             8360..9244
                     /locus_tag="ACHKIK_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFO9084036.1"
                     /translation="MENELIIDGQVIDLSETQENAEETIIQTESQPENESQDDNGKEM
                     ATDPEKTEETPEDYALRIGDEEIQLNADDNDHIDGQPAPQWVKDLRKGFKETQKENRE
                     LRRQLEEALAKPAEHQQPQPDAIPPKPTLESCDYDEQAFEQALTDWHEKKGRVEQQQQ
                     QKLRQQQEYQQRFQQRVEAHKQRAAKLPVKDYQEMEAIVLSELPPIQQEIIIHCADEG
                     SELLAYGLGKSQQLRQRVAAETDPIRAAFLLGQISKQVSLAPKPKKAIKPEPEVRGGG
                     ADAKQDEFNKLCPGAKIE"
     gene            9256..10527
                     /locus_tag="ACHKIK_002898"
     CDS             9256..10527
                     /locus_tag="ACHKIK_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFO9084037.1"
                     /translation="MPNNLDSNVSQIVLKKFLPGFMSDLVLAKTVDRQLLAGEINSST
                     GDSVSFKRPHQFSSLRTPTGDISGQNKNNLISGKATGRVGNYITVAVEYQQLEEAIKL
                     NQLEEILAPVRQRIVTDLETELAHFMMNNGALSLGSPNTPITKWSDVAQTASFLKDLG
                     VNEGENYAVMDPWSAQRLADAQTGLHASDQLVRTAWENAQIPTNFGGIRALMSNGLAS
                     RTQGAFGGTLTVKTQPTVTYNAVKDSYQFTVTLTGATASVTGFLKAGDQVKFTNTYWL
                     QQQTKQALYNGATPISFTATVTADANSDSSGDVTVTLSGVPIYDTTNPQYNSVSRQVE
                     AGDAVSVVGTASQTMKPNLFYNKFFCGLGSIPLPKLHSIDSAVATYEGFSIRVHKYAD
                     GDANVQKMRFDLLPAYVCFNPHMGGQFFGNP"
     gene            10570..10755
                     /locus_tag="ACHKIK_002899"
     CDS             10570..10755
                     /locus_tag="ACHKIK_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084038.1"
                     /translation="MDRMSVFLAADNESGHVQAVIAEKDFQFFERLGFVASVDELKPT
                     SKRGRKAADNGNSTDKG"
     gene            10730..11212
                     /locus_tag="ACHKIK_002900"
     CDS             10730..11212
                     /locus_tag="ACHKIK_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFO9084039.1"
                     /translation="MATVLTKGEIVLFALRKFAIASNASLTDVEPQSIEDGVNDLEDM
                     MSEWMINPGDIGYAFATGDEQPLPDDESGLPRKYKHAVGYQLLLRMLSDYSLEPTPQV
                     LSNAQRSYDALMTDTLVVPSMRRRGDFPVGQGNKYDVFTSDRYYPGDLPLIDGDIPNA
                     "
     gene            11221..12639
                     /locus_tag="ACHKIK_002901"
     CDS             11221..12639
                     /locus_tag="ACHKIK_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFO9084040.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPADSGFMQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDPEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYGLQQEHLLFTPLFKADNARCF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            12639..13487
                     /locus_tag="ACHKIK_002902"
     CDS             12639..13487
                     /locus_tag="ACHKIK_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFO9084041.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHESRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNSATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            13487..13942
                     /locus_tag="ACHKIK_002903"
     CDS             13487..13942
                     /locus_tag="ACHKIK_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFO9084042.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VYGQFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            13945..14637
                     /locus_tag="ACHKIK_002904"
     CDS             13945..14637
                     /locus_tag="ACHKIK_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO9084043.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            14647..16053
                     /locus_tag="ACHKIK_002905"
     CDS             14647..16053
                     /locus_tag="ACHKIK_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFO9084044.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIDRS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLASSSPEAMQSWLQNNAKELTRVGVDPNSVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQ
                     ARGQNLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGI
                     ETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQ
                     NALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNE
                     FKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEP
                     RNQVGSYTSKSGIQFTVE"
     gene            16053..17897
                     /locus_tag="ACHKIK_002906"
     CDS             16053..17897
                     /locus_tag="ACHKIK_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO9084045.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTRDEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDKPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDSLHEVLQKTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNALLRLANTKAGTPAYERALNNAANAIRPILSSQITAEQQ"
     gene            complement(17912..18397)
                     /locus_tag="ACHKIK_002907"
     CDS             complement(17912..18397)
                     /locus_tag="ACHKIK_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084046.1"
                     /translation="MKKSLLIIPLLLVGCAKVSDYQASCEQRYQKLSDMANCLDASVK
                     NDSRMASAPTPKLYVLAAKMLGKGVDEGKISDAQARLELQNLYVQLQSQEQAQQIAQS
                     QAFQQALLNYQAVNTMQAIEQKARQPVITQPYPTRVDTYTNCNSGFGNTVTCNSSSNI
                     R"
     gene            complement(18468..18734)
                     /locus_tag="ACHKIK_002908"
     CDS             complement(18468..18734)
                     /locus_tag="ACHKIK_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO9084047.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKMVFETLKTMYSNNKKET"
     gene            18856..20979
                     /locus_tag="ACHKIK_002909"
     CDS             18856..20979
                     /locus_tag="ACHKIK_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tailspike protein"
                     /protein_id="HFO9084048.1"
                     /translation="MSDITANVVVSMPSQLFTMSRSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVSQPIIINAAGYPVYNGQIAKFVTVQGHSMAVYDAYGAQQFY
                     FPNVLKYDPDQFKGNLLSEDHSLGDSLVMHNPQFVDSTSTILSKKLTIDLRTLTDYGF
                     AEGNTGAQNKAAFQKAIDDATLPTEIVIPEGAFIVDPGITIKNTVTMIRGAGAYQSRI
                     FSTGTAAPIITQQDGVITFCEFRDFGLDGNGYAANGISLTEANHIKIENIDVVNTNNN
                     AILVNGYSIDIIGCRLFQNAGNGINVGGYCNNINIINSRIYGNGAGGVLLTPAYAEGG
                     MSVRVNGNNIEQNKFYGLLAYGVKGLNLDANYWERNGEIGYPYSVPESITVRADIHLI
                     ANNFTLIPDLSKINDTVSIRGNQQTAIGYASALPNQDGFIFTNYAKNLTIENNQLLDA
                     SKVNNLLAMYHNNLSSKVTDRLYLANNTVNSIGYVGSYDPATQNPDTAHLIDIANREL
                     TANYLDRNMLLWTAASGTTGTLIKTQNIYAGNYSFLVTTGDRVWGRTIDLNKSPELKG
                     KFVWFGAWVNDQGSASKLMFIINGAGQTDSTAPLAGNGKWSYVSCGVYIYETDTAINV
                     GIRNYGSGNVLINSPSLCAYGMPSNALQVEKTTFYLSSVPTSGFWDIGERVINSAPAS
                     GQPKAWTCNIPGGPGTYSFLSEGNF"
     gene            21050..>21378
                     /locus_tag="ACHKIK_002910"
     CDS             21050..>21378
                     /locus_tag="ACHKIK_002910"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO9084049.1"
                     /translation="MERKTNKLDSIQVLRGIAVLLVIAFHFRVYLNGVYAQKDLGDIL
                     FGIGEVGVDIFFVISGFIITYSSMNKAKNRPMVFAAKRFFRLYPVYFIILTLAIYLNY
                     EEIYTSS"
BASE COUNT         6059 a         4851 c         5300 g         5168 t
ORIGIN      
        1 acgccgggcg caagatgcag agattgtcac ggtacaggcc gtgcggttga tatagcaaaa
       61 acagagcagt gggggagagt tgttgagaaa gagtgcggaa gatgcaaagg cgtcggctat
      121 tcaaggatgc cagcaagtgc cgcatatcgc gctgtaacga tgctaatccc aaaccttact
      181 caacccacct ggtcacgcac tgttaagccg ctgtatgacg ctctggtggt gcaatgccac
      241 aaggaagagt caatcgcaga caacattttg aacgcggtca cacgttagca gcatgattgc
      301 cacggatggc aacatattaa cggcatgata ttgacttatt gaataaagtt gggtaaattt
      361 gacccaacga tgggttaatt cgctcgttgt ggtattgaga taaaaagagg cggcgcttac
      421 taccgattcc gcccagttgg tcacttcgac gtatcgtctg gaactccaac catcgcaggc
      481 tgagaggtct gcaaaatgca atcccgaaac agttcgcagg taatagttag agcctgcata
      541 acggtttcgg gattttttat atctgtgtaa caggtaagag cattctccct tatggggctt
      601 ggcttaaatg caccgagtgc tcttatcgtt gtggcagcac aacgatagtt ttcgtcagag
      661 ttggcgactt tgcggttttt tagaaactga ccacaaagat aaatgcaaac gatgatgttg
      721 ttctgatggc ggcgtaatag cctgtaagtc agcaaggtct tccgactcct tgtaaacaaa
      781 ttcggcgcac tggcccggtg tgattaataa tgggcacaca acaggtaaga gcattaaaga
      841 actggcaaag agcttaacgg tctgcgaaag catttcttag tggcacaact ggccggtaca
      901 actgagtgct ctttccggtg tggtgaatgc gcaggctgat gcgcgcagga gagcttcgga
      961 agaacaaggt gcctgtatac aagccggaga tcagcgccgg ccaccacagc caaatccacc
     1021 cagagcaaaa ccgttgttca tccttaccat tcactcagta ttttgggcta caccatcagc
     1081 ccatttttta aagcgtactt ccaccaagaa ccagacctaa ccaactcatt gctgacactc
     1141 tgtggatacg gttgtctagt acgctttaaa aaagaaaacc cagcatcaat ggctgggctt
     1201 cgtgatatga gcggcatgta ttgttggcgc aatccacgcc tgatttgctc atgaatgcgg
     1261 tcacgaacaa acccgttaca aatcaaccgt aacccggatt tgttcaagcg accatatcca
     1321 taattcctaa tttgaacaga tccccttctg ggggtaagac atgaagatgc cagaaaaaca
     1381 tgacctgtta gccgccattc tcgcggcaaa ggaacaaggc atcggggcaa tccttgcgtt
     1441 tgcaatggcg taccttcgcg gcagatataa tggcggtgcg tttacaaaaa cagtaatcga
     1501 cgcaacgatg tgcgccatta tcgcctggtt cattcgtgac cttctcgact tcgccggact
     1561 aagtagcaat ctcgcttata taacgagcgt gttcatcggc tacatcggta ctgactcgat
     1621 tggttcgctt atcaaacgct tcgctgctaa aaaagccgga gtagaagatg gtggaaatca
     1681 ataatcaacg caaggcgttc ctcgatatgc tggcgtggtc agagggaaca gataatggac
     1741 ggcagaaaac cagaaatcat ggttatgacg tcattgttgg cggagagcta ttcactgatt
     1801 actccgatca tcctcgcaaa cttgtcacgc taaacccaaa actcaaatca acagccgccg
     1861 gacgttacca gcttctttcc cgttggtggg atgcctatcg taagcagctt ggcctgaaag
     1921 acttctctcc caaaagccag gacgctgttg cgctgcagca gattaaagag cgtggcgctt
     1981 tgccgatgat tgatcgcggt gatattcgtc aggcaatcga ccgttgcagc aatatctggg
     2041 cttcactgcc gggcgctggt tatggtcagt tcgagcataa ggctgacagc ctgattgcaa
     2101 aattcaaaga agcgggcgga acggtcagag agattgaggt atgagcagag tcaccgcgat
     2161 tatctccgct ctggttatct gcatcatcgt ctgcctgtca tgggctgtta atcattaccg
     2221 tgataacgcc atgacctaca aagagcagcg cgataaagcc acatccatca tcgctgatat
     2281 gcagaagcgt caacgtgatg tagcagaact cgacgccaga tacacaaagg agcttgctga
     2341 tgctaacgct actatcgaaa gccttcgtgc tgatgtttct gctggtcgta agcgcctgca
     2401 agtcgccgcc acctgtgcaa agtcaacgac cggagccagc agcatgggcg atggagaaag
     2461 cccaagactt acagcagatg ctgaactcaa ttattaccgt ctccgaagtg gaatcgacag
     2521 gataaccgcg caggttaact acctgcagga gtacatcagg actcagtgcc tgaaataatt
     2581 tttttgcaaa tcacaaagtc catttaatga gcctcgcgat gcggggcttt ttttacatct
     2641 gaatttcaca gcgcatctca cgcgcatatt acatcacccg agcctttcag aaagttgagc
     2701 ctgagaactg ccgtatatgg tggcgaccat ctcggggcgg cttttctgtg agacaggctc
     2761 actttctaaa aggtaaagac gctatgaatc atcaattggc taatctcgat ttccgggaca
     2821 tggtggttgt ttctggtgat cgcgtgatca caacctcccg caaggtagca gcttacttcg
     2881 acaagcagca tcaccacatc attcagaaaa tcgaaaagct agactgttcg gatgaatttc
     2941 taaccagcaa cttttcgcgg gttacctatg aacacaaggg taatcagtat gttgaatatg
     3001 aaatttccaa agacggtgcg atgtacatca tcatgtcgtt taccggcaaa aaagctgccg
     3061 ccatcaaaga ggcgtttatc aaagcattta attggatgcg tgacaggctg atggagatgg
     3121 ctcactcata ccaaagagag cacaacgagt taatgctgga gttcatgaag gaaaaggatg
     3181 ttgccagtat gtcaggacgc ttgctgaacc gctggggcag gatcaaaaaa ccgcaactca
     3241 tagcaagaat caaaaggctt gagcagcagg cgcaaatatc gatccccgga ctgccaaagt
     3301 gaccattcca aagcccatct acgggtgggc ttgataatga aaccgtgatt tacatcccca
     3361 caatccgggt atgtaaaaga tagttcaggc gagaacagat ttaactaaat ctgtgcacca
     3421 ccagttacgg cagtaccgcg aaacaaccca agccagtaag tggggaaata acactggcag
     3481 ccactgaaag atgaacctcc agccttatgg caaaaaagat tctttgtggt ggcgaactga
     3541 tggaaagaca tcctaatcaa gcaaccactc tacagggtca taattatgaa cgaccagcaa
     3601 atcgaaaaag aaatcgttga gaaaggcaaa acggcaccgc gagttacgcc agaccatatc
     3661 gaaggaatta ttgctcagga ggcatatttc acagcagagg atggtgcctt tggcaaagcc
     3721 ataaaagcga aacatactgg cggagaggta aactaccagt cgcacgaatc actttctctg
     3781 ctgacgttct gcgtcctggt gctgcgcaac ggcttcaccg tcaccggaga gagtgcctgt
     3841 gcaagtcagg aaaattttga tgcagaaatt ggtcggaaga ttgcccggca gaatgctgta
     3901 aacaaaatct ggatgctcga aggttacttg ctgaagcaga agctaagcga acagtagtta
     3961 ttacaaaagc cattccttac tgagtggctt tgataatggc ttatacccta cacgggataa
     4021 cttaactgat atccctttta acggataaac ggagccaaca atggcagaga ttattcccat
     4081 gactgaagaa cagaaattcc agttagagat ttacaaactg gtcatgaacc agaacgcagc
     4141 cgcggaggaa gcatttcagt tcattggcac tgacgagctg aagcttgagc tattcaaaat
     4201 tcacttccag tcaggtggcg ctaattcaga tatcacgacc cgcacaatcg aagcggtgcg
     4261 taaatcgaag gaagcgttag acctgttcac taccggagcg taaacatggc aactcaaggt
     4321 ttcgacaacc catccaaatt ccgcgatgaa tgggataagc aagcagaagg gaaataatca
     4381 atatggcgac tgagaaaaag aaaggtggtc gcccctctga ttatatgccg gaggtggcta
     4441 atgacatttg cgcattgctt tcctccggtg agagtctgcg caaagtttgc gaacgcccag
     4501 gaatgccgag caaaacatca gtttttcgct ggctggctga acatcaggag tttcgtgacc
     4561 agtacgcgaa ggcaacagag actcgggccg actctatttt cgaagagata ttcgaaattg
     4621 ctgacgacgt aatccctgat gccgccgagg tggcaaaggc aagacttcgc gttgataccc
     4681 gcaaatgggc gctggcccga atgaatcccc gtaagtatgg cgacaaggta actaacgagc
     4741 ttgtcggcaa agacggcggc gcaatccaga ttgaaacatc accgatgagc actctattcg
     4801 gaaaatgacc tcgattaatc ctatctttga accgttcatt gaggctcatc gctacaaagt
     4861 cgccaaaggc ggtcgaggta gcggtaagtc atgggcaatt gcgaggcttc ttgttgaagc
     4921 ggcgcgtcgg cagcctgtgc gtattctttg cgctcgtgag ctgcaaaaca gtatcagcga
     4981 ttcggtaatc aggttgcttg aagacaccat cgagcgtgaa gggtattcgg ctgagtttga
     5041 aattcagcgt tcaatgattc gtcatctcgg aacgaatgct gaattcatgt tctacggcat
     5101 caaaaacaac ccgacgaaga ttaaatcgct cgaaggcatt gatatctgct gggtggaaga
     5161 agcggaagcg gtaacgaagg aatcgtggga tatcctgatt ccaaccatcc gtaagccgtt
     5221 ctctgaaata tgggtaagtt tcaacccgaa aaacatcctc gacgatacct atcagcgatt
     5281 cgtcgtaaat cctcccgatg atatttgcct gctgacggtg aactacaccg acaacccgca
     5341 ttttcctgaa gtcctccgtc tggagatgga agagtgtaaa cgcagaaatc cgacactgta
     5401 tcgtcacatc tggcttggtg agccggtaag cgcaagtgat atggcaatca tcaaacgtga
     5461 atggcttgaa gccgcaaccg atgcgcacac gaaactcggg tggaaagcga aaggtgcggt
     5521 tgtttctgcg catgacccat cagatacagg gccagatgct aaaggttatg catcgcgtca
     5581 cggttcggta gttaagcgca ttgccgaagg tctgctgatg gacatcaacg agggtgctga
     5641 ctgggctact tcgctggcga ttgaagacgg cgctgaccat tacctgtggg acggtgatgg
     5701 cgtcggtgcg ggcctacgca gacagacaac ggaagcattc tccggcaaga aaattaccgc
     5761 cacgatgttc aagggcagcg aatcgccatt cgatgaagat gggccttatc aggccggagc
     5821 atgggctgat gaagtcgtac agggcgacaa cgttcgcact attggcgatg tattccgcaa
     5881 taagcgagcg caattctatt acgcgctggc tgacaggctg tatctgacat atcgggcggt
     5941 tgtccacggt gagtatgcag accccgacga catgctgagc ttcgacaaag aagcgatagg
     6001 cgagaagatg ctggagaagc tgtttgcaga actgacgcag attcagcgca aattcaataa
     6061 taacgggaag ctggagctta tgactaaggt cgaaatgaag cagaagctcg gtatcccatc
     6121 tcctaacctg gctgatgcgc tgatgatgtg tatgcattgc ccggcattgg tccgcgaaga
     6181 aacagaaata tacgttccct catcctccgg ttggtaaaca tggcagagac attagagaaa
     6241 aaacatgagc ggatcatgct caggtttgac cgcgcctatt ctccacagca ggaagtgcgc
     6301 gaaaagtgca ttgaagctac gaggtttgct cgtgtcccag gaggtcaatg ggaaggagca
     6361 acggcggctg gaactaagct tgatgagcag ttcgagaagt atcctaagtt tgaaatcaat
     6421 aaggtagcaa ctgaacttaa ccgcatcatt gcagaatacc gcaataacag aatcactgtt
     6481 aagtttcgtc ctggtgacag agaggcaagc gaagagttag ccaataaatt aaatggtctg
     6541 ttccgtgctg actacgaaga aaccgatggc ggtgaggctt gcgataatgc atttgacgac
     6601 gctgctactg gtggtttcgg ttgcttccgt ttgacgtcga tgctggtcaa tgaatacgac
     6661 ctcatggacg atcgtcagcg tattgctatt gaaccaatat acgacccgtc gcgctctgtg
     6721 tggtttgacc ctgacgctaa gaagtacgac aaatctgacg cgttgtgggc gttctgcatg
     6781 tattcgttgt cacctgaaaa atatgaggct gaatacggaa agaaacctcc tgcttctctg
     6841 gatgtaacgt ctatgaccag ttgggaatat gactggtttg atgaagatgt tatttacata
     6901 gcgaagtatt acgaagttcg taaagagtct gttggcgtca tcagttatcg acatccaatc
     6961 actggagaga ttgcaacata cgacagtgat caggttgaag atattgaaga tgaactggca
     7021 atagctggat ttcaggaagt ggcaaggcgc tcagtgaagc gccgtcgtgt gtatgtatcc
     7081 gtagtggatg gtgatggttt ccttgagaaa cctcgacgta ttcctggtga gcatatcccc
     7141 ctcatcccgg tttatggaaa acgctggttc attgatgaca ttgagcgtgt cgaaggacat
     7201 attgcaaaag caatggatcc acagcgtttg tataaccttc aggtatcaat gctggctgat
     7261 actgcagcgc aagaccccgg tcagatccct atagttggca tggagcaaat tcgtggactt
     7321 gagaagcact gggaggctcg caacaagaaa cgcccagcgt tcttgccgtt gcgcgaagtg
     7381 agagataaat ctggcaacat tatcgctgga gctaccccgg caggatatac acagcctgcg
     7441 gttatgaatc aggcattggc tgcattacta cagcaaacca gtgctgatat tcaggaggtt
     7501 acaggcggca gtcaggccat gcagcagatg ccaagtaata ttgctcagga aacggttaac
     7561 aacttgatga acagagcaga tatggcttcg tttatctatc tggacaatat ggcgaaaagt
     7621 cttaaacgcg ctggtgaagt atggctgtca atggcgcgtg aagtgtacgg ttcagagcgt
     7681 gaagtgcgca tcgttaacga agatggaagt gatgatatcg cggtcctgag cgcacaggtt
     7741 gttgacaggc aaacaggggc tgttgttgcg ttaaatgacc tttctgtcgg tcgatacgat
     7801 gtgacggttg atgttggacc aagctacaca gcacgacgtg atgcaacggt ttctgtactg
     7861 acaaatgtcc ttagctctat gcttccaaca gacccaatgc gcccggcaat tcagggtatt
     7921 attctggaca atatcgatgg cgaaggcctt gatgacttca aagagtacaa ccgaaaccaa
     7981 ctgctgatat ctggtattgc aaaaccacgc aatgagaaag agcagcagat tgttcaacag
     8041 gcgcaaatgg cagcacaaag ccagccaaat cctgaaatgg ttctcgctca ggcgcaaatg
     8101 gtagcagcgc aggcagaagc gcaaaaagca actaacgaaa ctgctcaaac tcaaatcaaa
     8161 gcatttactg cccagcagga tgcgatggag agtcaggcaa acactgtcta taaactggct
     8221 caagccagaa acatcgatga caaagcagtg atggaggcaa tacgccttct gaaagatgtc
     8281 gccgagtcac aacaacagca attccagtca ccaccacagt caccggcaga cttaatgccg
     8341 agttaaccag gagtaatcaa tggaaaacga actgatcatc gacggtcagg ttattgacct
     8401 gtctgaaaca caggaaaatg cagaagaaac catcatccaa acagagtcac agcctgagaa
     8461 tgaaagccag gatgacaacg gaaaagagat ggcaactgat cctgaaaaaa ccgaagagac
     8521 accagaagat tacgccttgc gtattggtga tgaagaaatt cagcttaacg ctgacgataa
     8581 tgatcacatt gacgggcaac ctgcaccgca atgggtgaaa gatcttcgca aaggcttcaa
     8641 agaaacacag aaagaaaacc gtgagttgcg acgccagctt gaggaagcat tagccaagcc
     8701 tgcggaacat cagcaaccac aaccagacgc tattccacca aaaccgactc ttgagtcgtg
     8761 tgattatgac gaacaggcgt ttgaacaggc attgactgat tggcatgaga aaaaaggccg
     8821 tgtcgaacag cagcagcaac aaaaactacg tcagcaacag gaataccagc agcgtttcca
     8881 gcaaagggta gaagcgcata aacaacgggc agccaaactt cctgtgaaag attatcagga
     8941 aatggaagcc attgttctta gtgagctacc accaattcag caggaaatca tcattcactg
     9001 tgcagacgaa ggctctgaac tactcgccta tggcttaggt aagagccagc aattacgcca
     9061 gcgtgtagcc gctgagacag atccaattcg cgcagcattc ctcttggggc agattagcaa
     9121 acaggtaagc cttgctccaa aaccaaagaa agccatcaag ccagagccgg aagtacgtgg
     9181 tggcggtgct gatgcgaaac aagacgaatt caacaaatta tgccccggcg caaaaatcga
     9241 ataaggaaaa gataaatgcc taacaatctc gacagtaacg tcagtcaaat cgttctgaaa
     9301 aaattccttc cgggttttat gtcagattta gttctggcga aaaccgtaga ccgtcagttg
     9361 ctggcaggtg aaatcaactc cagcactggc gatagcgtta gctttaaacg tccgcatcaa
     9421 ttctcatccc tccgtactcc cactggtgat atttcagggc aaaataaaaa caacctgatc
     9481 tcaggtaaag ctacggggcg tgtaggtaac tacatcactg ttgctgttga atatcagcaa
     9541 ctggaggaag cgatcaagct taaccagctg gaagaaattc tcgcgccggt tcgccagcga
     9601 atcgttaccg accttgaaac agagcttgct cacttcatga tgaataacgg tgcgttgtca
     9661 cttggtagcc ccaatactcc aatcaccaaa tggtctgatg ttgcgcagac ggcatctttc
     9721 ctgaaagacc tcggcgttaa tgaaggtgaa aactatgctg taatggatcc atggtctgca
     9781 cagcgacttg ctgatgcgca gactggtttg catgcttcag atcaattggt tcgtactgca
     9841 tgggagaacg cacagatccc aaccaatttt ggcggcattc gcgcactgat gtctaatggg
     9901 cttgcctctc gtacgcaggg ggcatttggc ggaacactga cagtcaaaac acagccaact
     9961 gttacctata acgcagttaa agactcatac cagttcactg taacattgac cggagcgaca
    10021 gccagcgtta caggttttct gaaagctggt gatcaggtca aattcaccaa tacctactgg
    10081 ctgcaacagc agaccaaaca ggcgttgtat aacggagcca caccaattag cttcactgca
    10141 acggttactg ctgatgctaa ttcagacagc agtggcgatg tgacggttac gctttctggt
    10201 gttccgattt atgacactac aaacccgcag tacaactctg taagtcgtca ggtagaggca
    10261 ggcgatgccg tatctgtagt aggcactgct agccagacaa tgaagccaaa cctgttctat
    10321 aacaagttct tctgtggact cggatctatc ccactgccga aactgcacag tattgattct
    10381 gctgttgcaa catatgaagg tttctccatc cgcgtacata aatacgcaga tggcgatgcc
    10441 aacgtgcaaa aaatgcgctt cgacttactg cctgcatatg tgtgctttaa cccacacatg
    10501 ggcggtcagt tcttcggtaa tccgtaataa caaggggctt acgccccttt tatgttttaa
    10561 ggaaacaata tggatcggat gagtgtattc cttgccgcag ataacgaatc cgggcatgta
    10621 caggccgtta tcgcagaaaa agacttccag tttttcgaaa ggttgggctt tgttgcctca
    10681 gttgatgaat tgaaaccgac cagtaagcga ggtcgtaagg cggcggacaa tggcaacagt
    10741 actgacaaag ggtgagatcg tcctttttgc gcttcgtaag tttgctattg cttctaatgc
    10801 atcgcttact gatgttgagc cgcaatcaat tgaagatggt gtaaatgatc tggaagatat
    10861 gatgtccgag tggatgatta accccggcga cattggttac gctttcgcaa ctggagatga
    10921 gcagccatta ccagatgatg agtcaggtct tccaagaaaa tacaaacacg cagtaggcta
    10981 tcagttattg ctgagaatgc tatctgatta cagccttgaa ccaactccgc aagttctcag
    11041 taacgcccaa cgctcatatg atgccttgat gaccgacact ctggttgttc cttcaatgcg
    11101 acgacgtgga gattttcctg tagggcaggg taataaatat gacgtgttca catctgaccg
    11161 atattatcca ggcgatctcc ctctgattga tggcgatatc ccaaacgcat aggtgaataa
    11221 atgcctattc agcaacttcc gcttatgaaa ggtgtcggca aagactttag aaacgccgac
    11281 tatatcgact atctgccagt gaatatgttg gctacaccca aagaaatcct gaacagcagc
    11341 ggatatcttc gctcattccc gggcattgcc aaacgttctg atgtgaacgg cgtatcgcga
    11401 ggcgtcgagt acaacatggc acagaatgct gtttatcgcg tgtgtggtgg caaactgtat
    11461 aagggcgaaa gtgaggtcgg tgatgttgcc ggaagtggtc gcgtatcaat ggcgcatggt
    11521 cgaacatcac aggcggtagg cgttaacggg caactggtcg aataccgcta tgatggcacg
    11581 gttaaaaccg tctcaaactg gcctgcagac agcgggttta tgcagtatga gttaggttct
    11641 gttcgcgaca ttacgcgttt acgtgggcgt tatgcgtggt caaaagacgg cactgattca
    11701 tggtttatca ctgaccctga agacgaatcg catcctgacc gctacagcgc acaatatcga
    11761 gccgagtctc agcctgacgg catcatcggc atcggaacat ggcgagactt catcgtctgc
    11821 tttggttcat cgacgattga atatttctcc ctgacggggg caaccaccgt tggtgccgct
    11881 ttgtatgtcg cacagccatc gctgatggtg cagaaaggca ttgccgggac ttactgcaaa
    11941 acgccgttcg ccgattcgta tgcgttcatc agcaatccgg caacaggtgc gccgtctgtg
    12001 tatatcatcg gctccggtca ggtatcacca atcgccagcg cgagcattga gaaaattctt
    12061 cgctcctaca ctgctgatga actggctgat ggtgtgatgg aatcgctgcg atttgatgcg
    12121 catgaactgc tgattatcca ccttccgcgc cacgtcctcg tgtacgacgc atcttcaagc
    12181 gccaatggtc cgcaatggtg tgtgttgaaa acaggcctgt atgacgatgt gtaccgcgct
    12241 atcgacttca tttacgaagg aaatcagata acgtgcggcg ataagctgga atcggttacc
    12301 gggaaattgc agttcgatat cagcagccag tatgggctac agcaagaaca cctgctgttt
    12361 actccgttgt tcaaagcgga taacgccaga tgctttgatc tggaggtgga atcatcgacg
    12421 ggtgttgctc agtacgctga ccgcctgttc ctctctgcaa ccactgacgg cataaattac
    12481 gggcgtgagc agatgattga gcagaatgaa ccgttcgttt acgacaaacg cgttttgtgg
    12541 aagcgagtag ggcgcatcag gaaaaatgtt ggcttcaaac tgcgcgttat cactaagtcg
    12601 cctgtcactc tgtctggcgc tcagataagg attgagtaat ggcggattcg aatctcaatg
    12661 tgccggtaat cattcaggct acacggctcg acacatcagt ccttccacgc aatatcttct
    12721 cgcagtcgta tctgctttac gttatcgcac agggtactga tgttggtaat gtggctaaca
    12781 aggccaacga ggccggacag ggcgcttatg acgcacaagt caggaacgat gagcaggatg
    12841 tgattctcgc tgaccatgag cagcgaattt ctgctgcgga agcaacgctt gttaatcatg
    12901 aggagcgaat cagccaggca gaatcaactc ttcaggaaca tgaaacgcga atcgctcaga
    12961 atgaaagcga tattgcgtcg cttgatacca gagttcagtc gctggaatcg caggtttcag
    13021 accatgaatc gcgcattgat gctctggagt atgccactac tcgcaagaag tcagaggttg
    13081 tttactctgg cgtatctgta accatcccga cagcgccgac caaccttgtt agcctgctga
    13141 aaacgctcac gccgtcatca ggctcgttgg caccattctt cgacaccgtt aacaacaaga
    13201 tggttgtgtt caacgagaac aaaaccttgt tcttcaagct gtcgatcgtc gggacgtggc
    13261 ccagcggaac cgccaacagg tcaatgcaac taacattttc cggctctgtt cctgacacac
    13321 tggtaagcag tcgcaactcg gcgacaacga ccgataacat cctgttagct acgttcttca
    13381 gcgtggataa agacggcttt cttgccacaa atggcagcac gttaaccatt cagtcaaatg
    13441 gtgcggcgtt tactgccaca accatcaaga taatcgcgga gcagtaatga ttcagttcaa
    13501 accaacgcga aacatcgacc tgatagaagc agtcggaaat caccctgaca ttattgccgg
    13561 aagcaacaac ggtgatggat acgactacaa gcctgaatgc cgttactttg aggttaacgt
    13621 gtacggtcag tttggcggca ttgtttacta tcaggaaatt cagccgctta cattcgattg
    13681 ccacgccatg tacctgccag aggttcgtgg attcagcaag gaaatcgggc tggcgttctg
    13741 gcgatacatt ctgactaaca ccaccgttca gtgcgtcaca tcgttcgctg cgcgcaaatt
    13801 ccgccacggt cagatgtact gcgcaatgat tggccttaag cgtgtaggaa ccatcaagaa
    13861 atacttcaaa ggcgtggatg acgtgacgtt ttacagcgcc acacgcgaag aactaatcga
    13921 cttcctgaat cacgggagat agctatgtta tatgcattta agctgggcag aaaactgcgc
    13981 ggcgaggaac cttattgccc tgaaaaaggc gggaaaggtg gcagctctga taaaagcgca
    14041 aagtatgcag cagaagctca gaagtatgcc gcagacctgc aaaatcagca gtggcagacg
    14101 atcatgaaaa accttgctcc gttcacgccg cttgcggagc agtatgttaa ccagcttcag
    14161 aatctttcca gtttagaagg tcaggggcag gcacttaatc agtattacaa ctctcagcag
    14221 tataaagacc ttgcaggtca ggctcgttac cagagtcttg ctgctgcgga ggcgacgggt
    14281 ggacttggtt cgacagccac aagcaatcaa ctggctacga tcgcgccgac actcggtcag
    14341 tcttggttat caaatcagat gagcaattac aacaatctgg caaacgttgg gcttggtgca
    14401 ctgcaaggtc aggcaaacgc cgggcagacg tacgccaaca acatgagcag cattgcacag
    14461 caaagcgcag cacttgccgc tgctaatgcc aataaaccat caagtcttca gactgcaatt
    14521 agcggcggca catctggtgc gattgccggt gcaggtcttg ccagcctttt gggaacatca
    14581 acgccttggg gcgctggcat tggtgctggt atcggattgc ttggctcgtt gttttaaggg
    14641 gtaatcatgg ctacttggca aggaacaaac ggcggattgt tggctggtat cggcggcgtc
    14701 aactcaaacg ctccgagcgt aaatgacatc ggcaatacgc ttcagcttat caggcagaac
    14761 aatgatattg atcgttcagg cgctaacaat gttgggctga ctgctttgca aggcctttca
    14821 ggtattgcag gggtgtttca gcaggaaaag caggctcagc ggcagaaaga atttcagcag
    14881 gcgtacgcta atgcttatgc gtctggtgat cgcggtgctt tgcgtcagtt ggctactcaa
    14941 tatccagacc agattgaatc cgttcgtaaa ggcatgggat tcattgatga agaccagcgt
    15001 aattctatcg gcaccttagc ggctggcgca cgccttgcgt catcgtctcc agaagcaatg
    15061 caatcatggc tgcaaaacaa cgccaaggaa ctgactcgcg tcggtgttga ccctaacagc
    15121 gttgctcaga tgtatcagca gaacccttca ggatttggtg agtttgttga tcaccttgga
    15181 atggctgctc ttggtccaat tgattacttc aatgttcagg acaagatggc tggtcgtgaa
    15241 attgaccgag gcagactggc agagacaatc cgcagcaatc aggctggaga agcacttcag
    15301 gcgagagggc aaaacctttc ctatcagtca gcaatgactg ggcacaatat cgcagcacaa
    15361 cgcttggctc tggatcagca agagttcggg tttaagatgc agcaagcgca ggaaaaggct
    15421 cagcagttga ttagcgaagc acctaagctg tcagtaaaca tggaaaaagg catcgagacg
    15481 gctgtaaaca atgctacagc atcatcaaac tcagccaatt ctatgagtgc gcttgctcaa
    15541 cagttcagag cagaaaaacc aacgacaggt ttgttcggta acgcacagaa catgttcgca
    15601 aaacttaccg gaagcgatac aacattgcgt gatttgcgca ttcgccaaaa tgcccttgtt
    15661 aacagtcagg ttcttaaatt cctacctccc ggcccagcaa cggataaaga cgttgagatc
    15721 gttcgacagg gtgcgccaac tgacatggat aaccctgaga cggtcgcaag atggcttgat
    15781 gcaatggcaa accttgagcg acgaaacgcg cagtttaatg agtttaaagc cgagtggatg
    15841 agcgcgaatg gcaaccctgg acaatcgcgt aatggcggtc agatattggg gttggatgtt
    15901 aaaaaaggtg aatcattggg gagtgccgtt aagcggtata tgtcaatgaa tactgacgca
    15961 gcgccagcac aagattcgac accttcagga gaaccacgga atcaggttgg atcatatacc
    16021 tcaaaatcag gcattcaatt tacggtggaa tgatgaaagt aactgcaaac ggtaagacat
    16081 ttacctttcc tgatggtacg agcaccgaag atattggcac cgccattgat gagtattttg
    16141 ctggtcaggc tgttcagcaa caaacagtta atcaggccaa taatgcacca acacgggacg
    16201 aaccatcatt gatgcaacaa gctggcgatt ggctcactgg tggtcaaagt gcagggcaaa
    16261 ttgcagaaca ggctggtcgt ggtctggtaa acataccatt tgacgtattg cagggtggcg
    16321 caagtctgat taatgcaatc agtcaggggc ttggtgggcc aaaagttttg gatgatgttt
    16381 atcgtccagt agacaaaccg acagacccat acgcacaagc cggtgaaaca attggtgggt
    16441 atttagttcc aggagttgga acggcaggaa gcatggctat tggatcgctg gcagaggccg
    16501 caaatcagaa aggcgatttc gcacaaaatg cagctaaaaa tgccggagtt aaccttgccg
    16561 ctcagggtgt tctttccgca gcagcaaagg gaatagggcg tggaataacg gctataaaag
    16621 gtgatattgc gccagaagtg gcgaagaaaa ttgccacatc agaatcgatg ggcgtgacac
    16681 caatgacatc tgatgttatc ccgccgaaaa atgctttcac tcgcggcctt actcaggatg
    16741 ccgagggggc tttgctcggg acgggctcaa agcgagcaga gcaatatgca acgcgtagta
    16801 agctggtaag caattatttt gaccgttttg gtgagtacaa ccctgatgat gtggtgaaat
    16861 ctctgaccac cacgttaagg gggcggaagg atgctgctgg cgctgttatc aatgacgtca
    16921 ccaataaaat gggtaatgcc gcagttgata ctacaaatac catgaatgct ctgaatacag
    16981 cgatcgcaag acaggaacgg cttgggactt cagccaatca aagcctgctt acatccttgc
    17041 gtaacctgcg tgaagaatta gcaaaccctg caactgattt ggatgttacg tttgatctct
    17101 tgcgtcagca cagaacagca tttagatcta atgttcaggg agatgctatg gtcttcccca
    17161 accaggcaaa agcagctacc aatatggtag agaatgcaat gtcaaaagac cttcgtaacg
    17221 cagttgcaaa aaacctcggt gcgtcagacg cagcaaaata ccttaaagca aattccgatt
    17281 atgcaaacgt ttataataag gtgcttaata aaaacattgc taacaagctc aacaaggcaa
    17341 gcagtgaagc cagtcctgaa cttataaata ctgttgtatt aagcagaaaa ccatctgacg
    17401 tgaaacgaat ctggagcgca ttggatgata aagggaaaga tgctatgcgt gcagcttacg
    17461 tcagcaaaat agcggaaaag gccggtgact ctccagccaa gttcatcact gaagttaata
    17521 agctgaaatc tcagtcaggt ggtgaaattt acaacactat tttttctgga aagcacatga
    17581 aagagcttga ttctcttcat gaagttctac agaaaacagc aaggtcagac accgcaaatg
    17641 tagtaactca gactgggcaa tcgcaagcca acaggataag gacgattggc gcaactgcga
    17701 ctcttggcgt atcaatgggg cttgaggctg gtttcggtgc aatgatgcgc ttgtatgagt
    17761 ccaaagcagc aaggaatgct ctcttacgtt tggcaaacac caaagcagga acaccagcct
    17821 atgaaagagc gctaaataat gctgcaaatg cgatacgccc tatactctca agccaaatta
    17881 cagcagaaca gcaataaaag ataagatgta actacctgat attactgcta ctgttgcatg
    17941 ttaccgtgtt tccaaatcct gaattgcagt ttgtatatgt gtcaacgcgt gttgggtaag
    18001 gttgagttat aacaggctgg cgcgcttttt gctcgatcgc ttgcattgtg tttacagcct
    18061 gataattcaa taaagcctgc tggaatgctt ggctttgtgc tatttgttgg gcttgttctt
    18121 ggctttgtaa ttgaacataa agattctgaa gctcaagtct tgcctgtgcg tcacttatct
    18181 tgccttcatc gacacctttc ccgagcatct ttgcagcaag gacatacagc ttaggtgttg
    18241 gtgctgatgc catgcgtgag tcgttcttca cactggcatc aaggcaatta gccatatcgc
    18301 taagcttttg atagcgttgt tcgcaacttg cttgatagtc acttactttt gcgcatccaa
    18361 ccagcagaag cgggataatt aacagtgatt ttttcatatg gttaactctc cttagttttt
    18421 cacaggatat catgaaggca atgccatttt agccggaaac tagatttcta tgtttccttt
    18481 ttattattgc tatacatggt cttaagcgtt tcaaaaacca ttttcttaac catatcagat
    18541 tgttgttctg ccatacgctc tgcatcatca atgtaaacgg atgcagagct ttgtttagcc
    18601 aacgattctt caatcgctgc aattatctct gagttcagcg acctgttatt catcttcgcg
    18661 cgctgtttaa ttttcgcgtg gagttcatgc ggaagtctca agtgaaactg cgcctcgtcg
    18721 tatttgctgt acatccttga tgcctcacca gttgggtgga atggcatcgt aacctactgg
    18781 ataaatactc aatagtacca tttcggtatg caatcacatc atggttgcat catatcattc
    18841 ttctggagca atgaaatgtc agatatcacc gcaaatgttg tggtcagcat gccttcgcaa
    18901 ctcttcacta tgtctcgctc ttttaaagcc gtagccaatg gaaaaattta tatcggtaaa
    18961 attgacactg acccggtaaa tccagaaaac cagattcagg tttatgtgga aaacgaagac
    19021 ggttctcacg ttcctgtttc gcaaccaatc atcattaacg ctgctggtta ccctgtatat
    19081 aacggacaga ttgccaagtt cgtaactgtg caaggccatt ctatggctgt ttatgatgca
    19141 tacggtgcgc agcagttcta ttttccgaat gtgctaaagt atgatccaga tcagtttaag
    19201 ggcaatctcc tgagcgaaga tcactcactt ggcgactctc tagtcatgca taacccacag
    19261 tttgtcgata gcacatcgac cattctctca aaaaagctaa ctatcgacct gaggacgctt
    19321 acagactacg ggtttgctga gggaaacacc ggtgctcaga acaaagctgc atttcagaag
    19381 gctattgatg atgctacgtt gccaactgag attgttatac cagagggggc gttcattgtt
    19441 gaccctggta ttacgattaa gaatactgtc accatgatac gaggtgctgg cgcatatcag
    19501 tccagaattt tctctacggg tacagcggca ccgataatca cgcaacaaga tggggtaatt
    19561 acgttctgcg agttcaggga ctttggtctt gacggaaatg gctacgcagc taacggcatc
    19621 agtcttactg aagccaacca cattaaaata gaaaatattg acgtggttaa tactaataac
    19681 aacgcaatat tagttaacgg ttactccatt gacatcattg ggtgccggtt gtttcaaaac
    19741 gccggtaacg gtattaatgt tggtggttac tgcaataata ttaatataat caacagccgt
    19801 atctatggta acggagccgg tggagtttta cttacgccag cttacgctga gggcggtatg
    19861 agcgtcagag ttaatggcaa caatatagag caaaataagt tctacggact tttggcttac
    19921 ggggttaaag gccttaacct tgatgctaac tattgggaac gcaacggtga gattggttac
    19981 ccgtatagtg tacctgagtc tattacagta cgtgcggata tccaccttat cgcgaacaat
    20041 tttacattaa ttcctgacct ttcaaaaatt aatgataccg tatctattcg cgggaatcag
    20101 cagaccgcga ttggctacgc cagcgcgcta cctaaccaag acggatttat tttcacgaac
    20161 tatgcgaaaa acctgacgat tgaaaataat cagttattag acgcatcgaa agtgaataat
    20221 ttattagcaa tgtaccacaa taatctttcg tccaaagtta ctgacaggct atatcttgcc
    20281 aacaacacag tgaactccat cggatacgtt ggtagttatg acccggcgac acaaaaccct
    20341 gacactgcgc atttaataga tattgcgaac agagagttaa ctgctaacta tcttgatcga
    20401 aatatgttgc tctggactgc ggcatctgga actacaggaa cattgataaa aactcaaaac
    20461 atctatgctg gtaattattc attcttagtt acgacaggag atagagtttg gggacgaacg
    20521 atagacctca acaaatcacc agaactaaaa gggaaatttg tatggtttgg tgcgtgggtc
    20581 aatgaccagg gatcagcgtc aaaacttatg tttataatta atggtgcagg acaaacagac
    20641 tctacagcac ctttagctgg taatgggaaa tggagctatg ttagttgtgg tgtgtatata
    20701 tacgaaactg atacagctat aaatgttggt ataagaaatt atggttcagg aaatgtactc
    20761 ataaactcac cttctctatg tgcatatgga atgccatcaa acgcgttgca agttgaaaaa
    20821 acaacatttt atctttcgtc agtacctaca tccggtttct gggatatagg tgagcgtgtt
    20881 atcaatagcg cacctgcttc aggacaacca aaggcatgga catgcaatat tccaggtggg
    20941 ccaggaacct attctttctt atcagaggga aacttttaaa agacctatgt attgaaatga
    21001 ttactttgat ctggtatgtt taaaaaaaat cagtaaaaca cattttgata tggaaagaaa
    21061 aacaaataag ttagactcta ttcaagtcct tagagggatc gcggtacttc tagtcattgc
    21121 gtttcacttt agagtatatt taaatggagt gtatgcccaa aaggacttgg gcgacattct
    21181 ttttggtata ggtgaggttg gtgttgatat attctttgtt ataagtggat ttatcattac
    21241 ttattcttca atgaataagg caaaaaaccg gccaatggtg tttgctgcta agcgtttttt
    21301 taggttgtac cctgtttact ttataatcct tactctggct atatacctca actatgagga
    21361 aatatataca tcatcaca
//
LOCUS       DAZGJW010000077        21054 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZGJW010000077 DAZGJW010000000
VERSION     DAZGJW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.077"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..133)
                     /locus_tag="ACHKIK_002911"
     CDS             complement(<1..133)
                     /locus_tag="ACHKIK_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO9084050.1"
                     /translation="MRDNGLNNMFFSLCQINDNHSFTSSSHTKKTKSYNYSKHHKNTL
                     "
     gene            complement(311..1960)
                     /gene="actP"
                     /locus_tag="ACHKIK_002912"
     CDS             complement(311..1960)
                     /gene="actP"
                     /locus_tag="ACHKIK_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO9084051.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1957..2271)
                     /gene="yjcH"
                     /locus_tag="ACHKIK_002913"
     CDS             complement(1957..2271)
                     /gene="yjcH"
                     /locus_tag="ACHKIK_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO9084052.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2472..4430)
                     /gene="acs"
                     /locus_tag="ACHKIK_002914"
     CDS             complement(2472..4430)
                     /gene="acs"
                     /locus_tag="ACHKIK_002914"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO9084053.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4822..6257
                     /gene="nrfA"
                     /locus_tag="ACHKIK_002915"
                     /pseudo
     CDS             4822..6257
                     /gene="nrfA"
                     /locus_tag="ACHKIK_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
     gene            6302..6868
                     /gene="nrfB"
                     /locus_tag="ACHKIK_002916"
     CDS             6302..6868
                     /gene="nrfB"
                     /locus_tag="ACHKIK_002916"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO9084054.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6865..7536
                     /gene="nrfC"
                     /locus_tag="ACHKIK_002917"
     CDS             6865..7536
                     /gene="nrfC"
                     /locus_tag="ACHKIK_002917"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO9084055.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7533..8489
                     /gene="nrfD"
                     /locus_tag="ACHKIK_002918"
     CDS             7533..8489
                     /gene="nrfD"
                     /locus_tag="ACHKIK_002918"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO9084056.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8509..10227
                     /gene="nrfE"
                     /locus_tag="ACHKIK_002919"
     CDS             8509..10227
                     /gene="nrfE"
                     /locus_tag="ACHKIK_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO9084057.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10220..10603
                     /gene="nrfF"
                     /locus_tag="ACHKIK_002920"
     CDS             10220..10603
                     /gene="nrfF"
                     /locus_tag="ACHKIK_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO9084058.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10600..11196
                     /gene="nrfG"
                     /locus_tag="ACHKIK_002921"
     CDS             10600..11196
                     /gene="nrfG"
                     /locus_tag="ACHKIK_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO9084059.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11538..12851
                     /gene="gltP"
                     /locus_tag="ACHKIK_002922"
     CDS             11538..12851
                     /gene="gltP"
                     /locus_tag="ACHKIK_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO9084060.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12929..13618)
                     /gene="yjcO"
                     /locus_tag="ACHKIK_002923"
     CDS             complement(12929..13618)
                     /gene="yjcO"
                     /locus_tag="ACHKIK_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO9084061.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13712..15859)
                     /gene="fdhF"
                     /locus_tag="ACHKIK_002924"
     CDS             complement(13712..15859)
                     /gene="fdhF"
                     /locus_tag="ACHKIK_002924"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15440..15442),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO9084062.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(16057..17523)
                     /gene="mdtP"
                     /locus_tag="ACHKIK_002925"
     CDS             complement(16057..17523)
                     /gene="mdtP"
                     /locus_tag="ACHKIK_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO9084063.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALLQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVALFAGLDLDLWGV
                     HRSAVAAAIGAHNATLAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFQGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17520..19571)
                     /gene="mdtO"
                     /locus_tag="ACHKIK_002926"
     CDS             complement(17520..19571)
                     /gene="mdtO"
                     /locus_tag="ACHKIK_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO9084064.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATMLEIGSLFLIYKWSYGEP
                     LIRLFIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19571..20602)
                     /gene="mdtN"
                     /locus_tag="ACHKIK_002927"
     CDS             complement(19571..20602)
                     /gene="mdtN"
                     /locus_tag="ACHKIK_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO9084065.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20621..20896)
                     /gene="ytcA"
                     /locus_tag="ACHKIK_002928"
     CDS             complement(20621..20896)
                     /gene="ytcA"
                     /locus_tag="ACHKIK_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO9084066.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4771 a         5543 c         5738 g         5002 t
ORIGIN      
        1 ttaacgtgtt tttatgatgt ttgctgtaat tatatgattt tgttttcttt gtatgtgatg
       61 aacttgtgaa agagtggtta tcgttaatct ggcaaagaga gaaaaacata ttatttaaac
      121 cattatcgcg cataaaatat ccttataatt aacaagaaag aaaaggcata ttctctgcat
      181 tatcattttc tacgactgtc aaaaatcgct cattttttaa tgagtttatt tgtttaatat
      241 tatgggaaaa ggtgatgcat gctggagagg aagagtattc cccggtcagg cgaccgggga
      301 aggggagaga ttaatgtgcg cggccttgct caacgccaaa gccggtctgg gagcggataa
      361 actgtgcacg gaacagttca cgctcgcgcg caccttccgc tgagttatcg gttgccgaga
      421 agaaccagat gccgaggaat gccacgctga tagagaacag cgccgggtat tcatacggga
      481 agatggcttt ttcgtgacca aggatctgta cccaaatcgt cgggccgagg atcatcagta
      541 ccactgcggt aatcagcccc agccagccac ccatcatcgc gccacgagtg gtcagtttcg
      601 accagtacat agaaagcaga atgatcggga agttacagct cgctgcgatg gcaaacgcca
      661 ggcctaccat gaaggcgatg ttctgattct caaacagcac gccgaggata atggcaatca
      721 cacctaaaat cagcacggtg attttcgata cccgcagttc ttcacgttcg gtcgcgcctt
      781 ttttgaagac gttagcgtac aggtcatgcg aaaccgctga tgcgcccgcc agcgtcagac
      841 cagcaaccac cgcaagaatg gtggcaaaag caaccgctga aataaaaccg aggaacaggt
      901 tgccgcccac tgcattcgcc aggtgaacgg ccgccatgtt gttaccacca atcagatggc
      961 ccgccgcgtc tttatattcc ggattcgcac caaccagcat gatcgcgccg aagccgataa
     1021 taaaggtcag aatatagaag tagcccataa acccggtggc gtagaacacg ctcttacgtg
     1081 cttcgcgggc atcgctgact gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac
     1141 caaacatcag tcccagaccc agagagagcg cggagatcgg atctttcacc agcccgcccg
     1201 gcttcatgat gtcgacacct ttcgggtgta ccgccatcgc ttcactgaac agattgttga
     1261 agctaaagcc gacgtgtttc atcaccataa aggccataaa gctggcaccg aacagcaaca
     1321 gcacggcttt gataatctgt acccaagtgg tcgccagcat gccgccgaac aggacgtaca
     1381 tcatcatcag cacgccgacc agcaccaccg caatgtgata gttaaggcca aacagcagct
     1441 cgatcagttt acctgcgccc accatctggg cgataaggta aagcgccacc accaccagag
     1501 aaccacaggc cgaaagaata cgaatcggcc cttgtttcag acggtaagag gccacatcgg
     1561 caaaggtgta gcgccccagg ttacgcagac gttcggcaat caggaacaaa atgatcggcc
     1621 agcccaccag gaagcccagc gagtaaatta agccgtcata gccggaggta aacaccagcg
     1681 cggagatccc caagaatgag gcggcggaca tatagtcccc ggcaatcgcc agcccgttct
     1741 ggaagccagt gatattgccg cctgcggtgt agtagtcgct acgagaacgt acgcgttttg
     1801 atgcccagta ggtaatgccg agcgtaaaca cgacgaaaat caggaacata ataatcgcct
     1861 gccagttcgt tggctggcgc tctacggccc cgctaatagc atccgcggcg ttagctgcga
     1921 aagggagtgt ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta cctcatgcag
     1981 gacttcatta ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga taccggtgag
     2041 aacaaaggag atcacaatca ctccaacacc aattggaata ccgcgtgtga cgctggtgtt
     2101 cggattcagc ggcgtgccca gccagccggg cgcgaaggcg atcagtaaaa taaagccgat
     2161 ataaactgcc agcataataa tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa
     2221 ctccctgaaa tgcgcattgt cttctatccg ctgataaatc gtaccattca tcacagattc
     2281 tccagaggta atgtagggat tgttttaatt cccgctccct tatgggagaa ggttaacgct
     2341 cgggtaaccc ttgccgaatg taggccggat aaggcgttat acgccgcatc cggcaatcaa
     2401 tgcctgatgc gacgctgtcg cgtcttatca ggcctacaaa ccgttaccga ctcgcatccg
     2461 gcaattgtgg gttacgatgg catcgcgata gcctgcttct cttcaagcag cttctcgact
     2521 acgccaggat cggcaagcgt cgaggtatcg cccaggttgc tggtatcgcc cgccgcaatt
     2581 ttgcgcagaa tacggcgcat aattttgccg gagcgggttt taggcaggga gtcggtccag
     2641 tgcagcacgt ctggcgtcgc cagcgggcca atctctttac gcacccagtt gcggacttct
     2701 gcgtacagtt ctggtgacgg ttcctccccg tgattaagcg tgacgtaggc gtagatcgcc
     2761 tgacctttaa tattgtgcgg aatacctact acggcggctt cggcaatctt cggatgcgcc
     2821 accagcgccg actcaatctc tgccgtcccc agacggtgac cggagacgtt cagcacgtcg
     2881 tccacacgcc cggtgatcca gtaatagcca tcttcatcgc gacgcgcgcc gtcgccgctg
     2941 aaatacatat ttttgaaggt ggagaagtag gtctgttcaa aacgttcgtg atcgccaaac
     3001 agcgtacgcg cctgacccgg ccaggagtcg gtgatcacca ggctgccttc ggtagcgcct
     3061 tccagcgggt taccttcgtt atcgaccagc gccggttgca cgccgaagaa cggacgtgtt
     3121 gccgaaccgg ctttcagctc ggtagcgcca ggcagcggcg tgatcatgaa accgccagtt
     3181 tcggtctgcc accaggtatc gaccaccgga catttctcgt tgccgatttt tttccagtac
     3241 cactcccacg cttccgggtt aattggctcg cccacggaac cgagaatgcg cagcgacgaa
     3301 cggtcggtac cctcgatcgc tttatcgcct tccgccatca gcgcgcggat cgccgtgggt
     3361 gcggtataga gaatattgac ctgatgcttg tccaccacct gcgccatacg ggcaggcgtc
     3421 ggccagttgg gtacgccttc aaacatcagc gtggtcgcac cgcaggccag cgggccgtac
     3481 agcaagtaac tgtgtccggt cacccagccc acatcggcgg tgcaccagta gatatcaccc
     3541 ggatgataat caaagacata tttaaaggtc agcgccgcgt acaccagata accgccggta
     3601 gtatgcagca caccttttgg cttaccggta gaaccggagg tgtagagaat aaacagcgga
     3661 tcttcggcgt tcatctcttc cgcctggtgc tgatcgctcg cttgctcaac caggtcgtgc
     3721 caccacaggt cgcgcccttc ctgccagtca attttcccgc cagtacgctt cagtaccacc
     3781 acatgctcta cgctggtgac gttcgggttt ttcagcgcgt catcaacgtt tttcttcagc
     3841 ggaatactgc gcccggcacg cacaccttcg tcggaagtga tcaccagtcg tgagttggaa
     3901 tcaataatgc gcccggcaac ggcttccggc gagaagccgc cgaaaatcac cgaatgcacc
     3961 gcgccaatgc gggcgcaggc cagcatcgca accgcggctt ccggcaccat cggcatataa
     4021 atcgccacca catcaccttt tttaatgccc agctcgagca gggtattggc gaagcggcac
     4081 acgtcgcggt gcagctcttt atagctgata tgtttgctct ggctggcgtc gtcgccttcc
     4141 cagatgatgg cggtgcgatc gccgttttct tgcagatggc ggtcaaggca gtttgccgcc
     4201 agattcagcg tgccgtcctc gtaccattta atggacacat taccgggggc aaaggaggta
     4261 tttttcacct tctggtacgg tttgatccag tcgagaattt ttccctgttc gccccagaag
     4321 gtatcaggta cgttaataga ttgttgatac atcgcctcgt actgctgagg gtttatcagg
     4381 caacggtctg cgatgttggc aggaatggtg tgtttgtgaa tttggctcat gcttttgttc
     4441 tccttgtagg atgttaataa tatgtggcat aagcgttaaa tgtaggggta ttggcagttt
     4501 tgtttagtat ttgggcgaca gatcacgcaa aagtagaatt gtgcaaataa acggcagggt
     4561 aatttttgaa ggtcaccaac aaaagttgat taattctttg aggaacatgc agttatgcat
     4621 gctgttgaaa aagaggaaga tactgactaa ctctaaagtg gtattttaca tgcacttaca
     4681 attgattaaa gacaacattc acagtgtggt tatatgttac acataggggc gagcaatgtc
     4741 atgacagtgt aggtgcggtt actgtcgtga aaacaataaa aaccgccatt gcaacaatgg
     4801 cgcaattcgg atgaagcccc tatgacaagg ataaaaataa acgcacgccg tatcttcagc
     4861 ttattgattc ctttttcttt ttcacttctg ttcacgctga acaaacggca gctcccgcaa
     4921 aacctgtaac tgtggaagcg aagaatgaaa cctttgcccc gcagcatccc gatcaatatc
     4981 tctcctggaa agccacctcg gaacagtcag agcgtgttga cgccctggcg gaagatccac
     5041 ggctggtgat cctgtgggcg gggtatccct tctcgcgcga ttacaacaag ccgcgtggac
     5101 atgcctttgc tgtgaccgat gtgcgtgaaa ccctgcgtac cggtgcgccg aaaaacgctg
     5161 aagatggtcc gctaccgatg gcgtgctgga gttgtaaaag cccggatgtg gcgcgtctga
     5221 tccagaaaga cggcgaagat ggctacttcc acggtaagtg ggcgcgcggc ggcccggaaa
     5281 tcgtcaacaa cttaggttgt gccgactgcc ataacaccgc ctcaccagag ttcgccaaag
     5341 gcaaaccgga gttaaccctt tcccgtccgt atgcggctcg cgcgatggaa gccattggta
     5401 aaccttttga gaaagccgga cgtttcgacc agcaatcgat ggtttgcggt cagtgccatg
     5461 tggagtatta cttcgacggc aaaaacaaag cggttaaatt cccgtgggat gacggcatga
     5521 aagtcgaaaa tatggagcag tattacgaca aaattgcctt ctctgactgg actaactccc
     5581 tgtcgaaaac gccaatgctg aaagcgcagc acccggaata tgaaacctgg acagcgggca
     5641 ttcacggtaa aaacaacgtg acctgtatcg actgccatat gccaaaagtg cagaacgccg
     5701 aaggcaaact ctacaccgac cataaaattg gtaatccgtt tgataacttc gcccagactt
     5761 gtgcgaactg ccatacccag gacaaagctg ctttgcaaaa agtggtcgcg gaacgtaagc
     5821 agtcgattaa cgacctgaaa atcaaggttg aagatcaact ggttcacgct cacttcgaag
     5881 cgaaagcagc gctggatgca ggcgcgacgg aagccgaaat gaagccgatt caggacgata
     5941 tccgtcatgc ccagtggcgt tgggatctgg cgatcgcttc ccacggtatt catatgcacg
     6001 caccggaaga aggtctacgg atgctcggta cggcgatgga taaagcggcg gatgcacgca
     6061 ccaaactggc gcgcctgctg gcgaccaaag gcatcaccca tgaaatccag atcccggata
     6121 tctcgaccaa agagaaagcc cagcaggcca ttggcctgaa catggaacaa atcaaggccg
     6181 agaagcagga cttcatcaaa acggtgatcc cgcagtggga agaacaggca cgtaaaaatg
     6241 gtctgttaag ccaataaccc cgttccgcct cgcaaggggc ggaaaacaca atggagtgaa
     6301 tatgagcgta ttacgttcgt tattaactgc cggggtgctg gcgtcgggct tgttgtggag
     6361 cctgaacgga attaccgcta cccctgcggc gcaggcatct gatgatcgct acgaagttac
     6421 ccagcagcgt aacccggatg ccgcctgtct ggactgtcat aaaccagata ccgaaggtat
     6481 gcatggaaaa catgcttccg ttatcaaccc gaataacaaa ctgccggtca cctgcaccaa
     6541 ctgtcacggc cagccatcac cgcaacaccg cgaaggggtg aaagatgtga tgcgctttaa
     6601 cgagccgatg tacaaggttg gagagcagaa cagcgtctgt atgtcctgtc atctgccgga
     6661 acagttgcaa aaagcgttct ggccgcacga tgtccacgta accaaagtgg cgtgcgccag
     6721 ctgccattcc ctgcatccac agcaagatac gatgcagacg ttaagtgaca aaggacggat
     6781 taagatttgc gtcgattgcc acagcgatca gcgcaccaat ccgaacttta acccggcgtc
     6841 tgttccgttg cttaaggagc agccatgacc tggtctcgtc gccagtttct caccggcgtc
     6901 ggcgtgctgg cagccgtcag cggcaccgca gggcgcgtgg tggcgaaaac gttgaatatc
     6961 aatggggtgc gttacggcat ggtgcatgac gagtcgttat gcatcggctg taccgcctgt
     7021 atggatgctt gtcgggaagt gaacaaggtg ccggaaggcg tctcgcgcct gacgattatt
     7081 cgtagcgagc cgcagggcga atttcctgat gtgaaatatc gcttcttccg taagtcttgc
     7141 cagcactgcg atcatgcgcc gtgcgttgac gtctgcccga ccggtgcgtc ttttcgtgat
     7201 gccgctagcg ggattgtcga tgttaatcct gatctctgcg tcggttgtca gtactgcatc
     7261 gccgcctgtc cgtaccgcgt gcgctttatc catccggtca cgaagacggc ggacaaatgc
     7321 gatttctgcc gtaaaaccaa tttgcaggcc ggtaagctgc ccgcgtgcgt tgaggcttgc
     7381 ccgacgaagg cgctgacgtt tggcaatctg gacgatccta acagtgagat ttcgcaactg
     7441 ctgcgccaga agcctactta ccgctacaag ctggcgctgg gaaccaaacc gaagctgtac
     7501 cgcgtaccgt ttaaatacgg ggaggtgagc caatgacgca gacttccgca tttcattttg
     7561 aatcgctggt gtgggactgg ccgattgcca tctacctgtt tttgattggt atttctgccg
     7621 gtctggtgac gctggccgtg ctgttacgtc gcttctaccc gcaggcgggc ggtgcagaca
     7681 gtacgttgct gcgcaccacg ctgattgtcg ggccgggcgc ggtgatcctc ggtctgttga
     7741 tcctcgtctt ccacctgaca agaccgtgga ccttctggaa gctgatgttc cactacagtt
     7801 ttacctcggt gatgtcgatg ggggtgatgc tgtttcagct ctacatggtg gtgctggtgc
     7861 tgtggctggc gaaaatcttt gaacatgatt tgcttgccct gcaacaacgc tggttgccga
     7921 agctggggat cgtgcaaaag gttctgagcc tgctgacgcc cgttcatcgc ggactggaaa
     7981 cattgatgct ggtgttggcg gtgttgttgg gggcttatac cggctttctg ctgtcggcgc
     8041 tgaaatcgta tccgttcctc aataacccga tcctgccggt gctgttcctc ttctccggca
     8101 tctcgtccgg tgcggcggtg gcgctgatcg ccatggcgat acgccaacgc agtaacccgc
     8161 attccacgga agcgcagttt gtacaccgta tggaaatccc cgtggtatgg ggtgaaatct
     8221 tcctgctggt ggcatttttt gtcggtctgg cgctgggcga tgacggtaaa gtgcgtgcgc
     8281 tggtggcggc attaggtggc ggtttctgga cgtggtggtt ctggcttggt gtcgccgggc
     8341 tggggctgat tgtgccaatg ttgctcaaac cgtgggtcaa tcgcagttcc ggcattcctg
     8401 ccgtgctggc ggcgtgtggg gccagtctgg tcggcgtgtt gatgctgcgc tttttcattc
     8461 tctacgccgg acagttgacg gtggcgtaag ccagaaaaga ggtggtttct ggacgtattc
     8521 cttcctgaag tcggttttct ggcgttgttg ttaagtctcg gggtcaacgt gttgaccccg
     8581 ttgacggcct ttgcgggagt gcggttgcgc tggcctgcca tgatgcgact cacttgcatc
     8641 ggcattctgg cgcagttcgc gctcctgctg ctcgcctttg gcgtactgac gtattgtttt
     8701 ctcatcagcg atttctcggt catttatgtc gcgcaacata gctatagcct gctgtcgtgg
     8761 gaactcaagc tggcagcggt gtggggcggc catgaaggtt cgctgctgct ttgggtgctg
     8821 ctgctttccg cctggagcgc gctgtttgcc tggcattatc ggcagcaaac cgatccgcta
     8881 tttccgctga cgttagccgt tttatctctc atgctcgccg cactgctact gtttgtggtg
     8941 ctgtggtccg atcccttcgt gcggatattt ccaccagcaa tcgaaggccg cgatctcaat
     9001 ccgatgctgc aacatcccgg tcttatcttt catccaccgc tgctttacct tggctatggc
     9061 ggtttgatgg tagcggcgag cgtggcgctg gcgagtttac tgcgcggcga gtttgatggt
     9121 gcctgcgcca gaatttgttg gcgctgggca ctacctggct ggagcgcatt aacggcgggg
     9181 atcatcctcg gttcctggtg ggcctattgc gaactgggct ggggcggctg gtggttctgg
     9241 gatccggtgg aaaacgcctc tttattaccc tggctttctg ccactgcgct gctgcacagt
     9301 ttgtccctga cacgccagcg ggagattttc cgccactggt cgctgttgct ggcgatagta
     9361 actctgatgc tgtcgctact gggtacctta attgtccgtt ctggcattct ggtttcggtt
     9421 catgcgttcg cactggataa cgtccgcgcc gtgccgttgt tcagcctgtt tgcactgatt
     9481 agccttgcgt ctctggctct gtatggctgg cgagcgcggg acggtggtgc ggtggtgcgt
     9541 ttttcggggt tatcgcggga aatgttaatc ctcgctacgc tgttgctgtt ttgcgcagtg
     9601 ctactgatcg tgctggtggg aacgctttat ccgatgattt acggcctgct gggctgggga
     9661 cgcctctccg ttggcgcgcc gtattttaac cgcgcgacgt taccgtttgg cctgttgatg
     9721 ctggtggtga ttgtgctggc gacgtttgtc tctggcaaac gcgtgcagct tccggcgctg
     9781 gtagctcatg cgggcgtgct gttatttgcc gcggggatcg tggtttccag cgtcagccgt
     9841 caggagatca gcctgaattt acagccgggt cagcaggtga cgctggcagg atacaccttc
     9901 cgttttgagc gcctcgatct gcaagccaaa ggcaattaca ccagcgaaaa agcgatagtg
     9961 gcactgtttg accatcagca acgcattggt gaactgatgc cggagcggcg tttttacgaa
    10021 gcacgtcgtc agcaaatgat ggaaccgtca attcgctgga acggcatcca tgactggtat
    10081 gcggtcatgg gtgaaaaaac cggagcggat cgttacgctt ttcgcttgta tgtacaaagc
    10141 ggtgtgcgct ggatctgggg gggaggattg ttgatgattg cgggcgcatt gttaagcgga
    10201 tggcggggga ggaagcgcga tgaataaagg acttctcacg ctactgctgt tatttacctg
    10261 ttttgcgcgc gctcaggtcg tagacacctg gcaattcgcc aatccgcaac aacagcaaca
    10321 ggcgttaaat attgccagcc agttacgttg tccgcagtgc cagaatcaaa acttactgga
    10381 atccaacgcg ccggtggcag tcagtatgcg ccatcaggtt tacagcatgg tggcggaggg
    10441 gaagaacgaa gtcgaaatca ttggctggat gaccgaacgc tacggagatt ttgttcgcta
    10501 taacccaccg ttaacgggtc agacgctggt gttgtgggcg ctgccagtgg tgttgttact
    10561 gctgatggca ctgatcctct ggcgagtgag ggcaaagcga tgaaagagcc caaaataccg
    10621 gtgaaaatgc tgacaacact cacgattttg atggtatttc tctgtgtcgg cagttatctg
    10681 ttaagtccaa aatggcaggc ggtacatgcg gaatatcagc gtcagcgcga tccgctacat
    10741 cagtttgcca gccagcaaac cccggaagcg cagcttcagg cattgcagga taaaatccgc
    10801 gctaatccac aaaacagcga acaatgggcg ttactgggcg agtactatct gtggcaaaac
    10861 gattacagca attcgctgct ggcgtaccgt caggcgttgc aactacgtgg tgagaacgct
    10921 gaactgtatg cggcgctggc gacggtgctt tattaccagg ccagccagca tatgaccgcc
    10981 cagactcgcg caatgatcga caaagccctc gcgctggact ctaacgaaat caccgctctg
    11041 atgctgctgg cttccgatgc gtttatgcag gcgaattacg cgcaagccat cgaactatgg
    11101 caaaaagtga tggatctcaa ctcaccccgg atcaaccgga cacagctggt cgagtcgatt
    11161 aatatggcga aattgttgca gcggagatca gattaatcat ctggtgatga agattatgtt
    11221 attgagttgt ataaaaaata atcgaatgaa catatgccaa aaataatcac caatcagtat
    11281 tattgcagat taaacaaata aaaatctttc cataacaaat ggttattcat taatcctgcc
    11341 ataaactcgc gttattatgc attaatgcag cgaaaagctc tgttgttaaa gggttgcgca
    11401 acataccgcg caaatgatac tgatcataag cgttaaaaaa atctacaaac caacgcaaca
    11461 caattcatgc cctggcagta tgtcacgttc tcgcgtttct gaacggggaa cggcgctcca
    11521 ttgaggaagt tattctgatg aaaagtataa aattcagcct ggcctggcag attctgtttg
    11581 ctatggtgct gggcattctc ctgggaagct acctgcacta ccatagcgac agccgcgact
    11641 ggctggtcgt caatttgctc tctccggcgg gtgatatctt catccatctg atcaaaatga
    11701 ttgttgtgcc gattgtgatc tccacgctgg tggtgggtat cgcgggtgtt ggtgatgcca
    11761 aacagctcgg gcgtattggc gcgaaaacca ttatttactt cgaggtgatc accaccgtcg
    11821 ccatcatttt ggggatcact ctggcgaacg tcttccagcc cggtgccggg gtggatatgt
    11881 cgcagctggc gaccgtcgat atctcgaaat atcagagcac tacggaagcg gtacaaagca
    11941 gttcccacgg cattatgggc acgattttgt cgctggtgcc gacgaacatt gtggcgtcga
    12001 tggcgaaagg cgaaatgctg ccgatcatct tcttctcggt gctgtttggt ctggggcttt
    12061 cctccctgcc cgcgacgcat cgtgaaccgc tggtgaccgt gttccgctcc atctctgaaa
    12121 ccatgtttaa agtgactcac atggtgatgc gttatgcacc ggtgggtgtg tttgcgctga
    12181 ttgcggtgac ggtggctaac tttggtttct cttctctgtg gccactggcg aaactggtgc
    12241 tgctggtgca tttcgccatt ctgttcttcg cgctggtagt gctgggaatt gtggcgcgtc
    12301 tgtgcgggtt aagcgtctgg atcctgattc gtattctgaa agatgagctg attctggcgt
    12361 actccactgc cagctctgaa agcgtactgc cgcgaattat tgagaagatg gaagcctacg
    12421 gagcaccggc gtcgatcacc agcttcgtgg tgccgaccgg ttactctttt aaccttgatg
    12481 gttcgacgct gtatcaaagt attgccgcta ttttcatcgc tcagctgtat ggcattgacc
    12541 tgtccatctg gcaggaaatc attctggtgc tgacgctgat ggtgacctcg aaagggattg
    12601 ctggcgtgcc gggcgtgtcg tttgtggtgc tgctggcaac gctgggtagc gtaggtattc
    12661 cgctggaagg cctggcgttt attgctggtg ttgaccgtat cctcgacatg gcgcgtactg
    12721 cgctgaacgt ggtgggtaat gcgctggcgg tgctggtgat tgccaagtgg gaacacaaat
    12781 ttgaccgcaa aaaggcgctg gcttatgagc gtgaagtgct gggcaaattt gataaaactg
    12841 cggatcaata attgaagatt gccggggtta tccacccggc aatgtgtgga tgcctgatgc
    12901 gacgctggtg cgtcttatca ggcctacctt aaccgttggt taatttttca aactcttcac
    12961 acccggtatc aaacccttcc atacagctca ggttcaacca gtgcagcgcc ttttgtttgt
    13021 tcttctcgat aaaaccttcc tcaccgttta agaacatcat tcccgcccag tactcggaat
    13081 aaccggttcg ggaaattgca gagctgcgtt tgaaatacca ggttgccttg tcatcgtcgg
    13141 ccttaatgcc cacgccgttg gcgtaaatca aaccaagcag catttgggca tcgacggcgg
    13201 agtcattctc cagatcttcc gaggcgtttt ctaacagcga aatcgccttt ggataatccg
    13261 gtttacccgc ttgagtattt accagaatat gcgccagcgt tacttcacct tctttactgc
    13321 ccgcttgcgc cgctttctct gcaagaactt ttgcttgtgg atagtccaga ctgaccggat
    13381 tggtgatttt gatctgcgcc agcagcgcgc aggcatcagc atcaccgctg tcggcggctt
    13441 tctgtgccca atattcggct ttgctcaaat cgccggagct aaaccagctg tcggcaagaa
    13501 aatattgtgc gcgtcggtcc ccggcctctg ctgcctttaa atactggctg ccaggctcgg
    13561 agtcgttcgc gtgggcaaag aatgtcaaaa acaacatcaa tgcgataatt tttttcattt
    13621 tgataatacg attgggtagg gagtaaccag tataaagagt tcgaatggat aaaaaaacag
    13681 cctccgaaag gaggctgtag aaaggactgt attacgccag tgccgcttcg cgcaggcgag
    13741 ttttcaactt gttgtactcg tcaatcacgt actgctcggc ggcgcgctga tcggcgatcg
    13801 gctcgacgcg cacggcgcag tatttgtatt ccggcgtttt cgtaatcggg cttaagtttt
    13861 cggtaaccag ctcgttacag gcaccaatcc accactggta ggtcatgtaa atcgcccctt
    13921 tgttcggacg atcgctgacc tgcgcacggg tgataatttt gcctttacgc gagtgcaccc
    13981 aaaccaatgc ctcatcttca atacccagac gtttggcgtc ttcggtattg atttgtgcgt
    14041 agccaggttc atcagccagc gccgccagtg ccgcacagtt accggtcatc gaacggcaag
    14101 agtagtgacc aacttcacgc accgttgaca gtaccatcgg gtactcgtcg gtgagtttgt
    14161 cgattggcgc tacccagtcg caggtgaaga actgtgccag accgttcggg gtatcaaact
    14221 tctctttaaa cagataagaa gtcccctgat cggcatctga agtatcgcgg caaggccact
    14281 gaatgaagcc cagttcgccc attttctcgt aagtcgcacc gtagaaatcc gggcacagat
    14341 gacgcaactc atcccagatc tcctgggtgt tgttgtagtg catcggataa cccatacggg
    14401 tggcgatttc actgatgatt tgccagtccg ttttcagatc ccatttcggt tcaactgctt
    14461 tgaagaaacg ctggaagcca cggtcagccg cagtaaacac gccttcatgc tcgccccacg
    14521 acgttgacgg caaaatgaca tccgccgccg acgcggtttt ggtcataaag atgtcctgaa
    14581 cgataaccag ttccagatct tcaaaggctt tacgtactgc cgacagctcc gcgtcagttt
    14641 gcagcggatc ttcgcccata atgtacgcgg cacgcacttc gccatgcgct gcgcggtgcg
    14701 gcagctcgct gatgcgataa ccggtatgag caggcaggct ttccacgccc caggctttgg
    14761 cgaatttctc gcggttagcc ggatctttca cgtactggta tcccggatac gtatccggca
    14821 gcgcgcccat atcgcaggca ccctgcacgt tgttctgacc acgaaccggg tttacacccg
    14881 catgcggctt accgaggtta ccggtcagca tcgcgaggct ggtcagagaa cgcacggttt
    14941 ccacgccctg gtagaactgg gttacaccca tgccccacag gatggcggcg cttttcgcct
    15001 gggcatacat ccgtgccgcc tgacgaatct cactggcgct gacgccggtg atatcttcaa
    15061 ccgactccgg cgtgtagcct tcaacgattt tacgatactc ttcaaagcct tctgtacggg
    15121 aagcgacgaa cgctttgtcg tacagatttt cttcaataat gacatggccc atcgcattca
    15181 acagcgcgat gttcgagccg tttttcagtg caatgtgcat gtcagcaatg cgcgcggttt
    15241 caattttgcg cggatcgcag acgataattt tcgccccgtt acgtttagcg ttaattacgt
    15301 gattcgccac gattgggtgg gaatccgccg ggttgtaccc gaaaacgaac actaaatcgg
    15361 tattatcaat ttcgttaata gcattgctca ttgcgccatt accgaccgat tggtgcagac
    15421 ctgcaaccga tgggccgtgt cagacacgag cgcagcagtc aacgttattg gtaccaataa
    15481 cggcgcgcgc aaatttttgc attacatagt tggtttcgtt acccgtacca cgcgaggagc
    15541 cggtcgtctg gatggcatcc ggaccgtact tctctttgat ggcgctcagg cgctcggcaa
    15601 cgtaattcag tgcctcatcc caggaaacag gttcgagttt gccgccacgc tggcgacgga
    15661 tcatgggggt tttcaggcgc ggggtcagga tctgggtatc gttaatgaaa tcccagccat
    15721 aataaccctt cagacacagg gtaccctggt tggttttccc ctgcgctgcc tccgcccgga
    15781 cgattttgcc gttatcgacg accaggttga ttttgcaacc tgatgcgcaa taggggcaaa
    15841 ccgtgacgac ttttttcatc ggtctcgctc cagttaatca aatcacgcat acgcgctctc
    15901 gactacagta tgcatctttt atgccacatt ttatttgggg tcattccctg atattacggg
    15961 cactatttat tcaaaactct gacgaaaaac aggctgtcgt cagttttgac gtgacgaaac
    16021 gaaataccgc gtgacagcca tcacgcggca gacattttat tttttctcga cgacgggagc
    16081 cgcctgatac ccgccgccca gcgatttcat caactgaatg ctttggatca cccggcggct
    16141 gtccagcatc agtaatgaca tctcttcggc aagtactggc aaccgggctt cagtggcctg
    16201 taagcggctg gttaagccgc gctgataggc ggcctcggca gcgcgctggg tgaagcgcgt
    16261 tgcttccacg cgttcagcct gcatttctcg ctcgtcgttg agcgtttgca gacgcgtgcc
    16321 gttgacggca acgtcacgca ccgcgttcag tactgactgg ttgtaacgtt caatcatcat
    16381 gttgctggcg gcgcgcgtgc cttcgagatt ggcattcaac cgtccaccgt caaacagcgg
    16441 caatttcaga cccgggatga agttgaactg gcgactggtt tttttgaata aggtatccag
    16501 gtggatggca tcaaggccga aaaacgcttt gatatcaaag ctcgggtaga acagcgcccg
    16561 cgcggaatcc acctgatcta atgacgcctg aacataccag cgcatggctt gcagatccgg
    16621 gcgtctggcg agcaactcat aagagagtgt cgccggaatg ccggtctgga ctcgtggtaa
    16681 tgccaccggt ttgatctccg gcatatcgct ggcccccgcg ccaatcaatg cacgcagaga
    16741 ttctcgcgtt tcggtgattt gccctttgac ggcagcaatt tgtttatcga ccgccagaat
    16801 ctgtgcccgc gcgccctgga aaggcacttg tgcttccaga ccgtgcgcca ctttactctg
    16861 gtgcgctttc accgcgtaat caatcacatc gcgagtttgt tctaacagat cgagcatctg
    16921 atagctggcc tgcatactgt aataaagctg cgctacgccc gtggtcagcg atagctctac
    16981 tgctgcggtt tctgccagcg tggcattatg cgcgccaatg gcggcggcaa ccgctgagcg
    17041 atgcacaccc cacaaatcaa gatccagtcc ggcaaacaaa gctactgtgg cttccgtata
    17101 gtacggcccg tccataccca gtgcgggcgc atccatcgca taagggctta aaaagccgtt
    17161 cgccgagacg cgttggcggt tgagcatccc taacgctgcg acctgtaatt gtgaaccggc
    17221 atctaacaaa tcggcctggg actgcgcttt ttcttcccgc agtttcgctt cggcgagggt
    17281 gtgtgaacca cttagcgtcc gttggagcag cgcatccagc tgcgggtcat tgagttgttt
    17341 ccaccactgc gcctgcggcc agccggagct ggcaagatga atatcgtcgg ccagtttgat
    17401 ttgttccggt ttgagctgtt gatgaggtgc agaatcctta cgtaccaggg cacagccgga
    17461 aatcagcgtc gtgctgccga gaatgctgca caacagcaga cgtgaaagtt gacgattgat
    17521 cattgcgtgg ctccttgtgc ctgttccgtg gcgggcgtta atgccggggc tgtccagtct
    17581 ggcaagcggg cgaaaagctg gcagacgtgt tgctcctgtt ttaataaggt gggcaggatg
    17641 gcctgcgagg cgggtgtctc ttcaagcgtt atttgaggag aacggctgag tgcggttgcc
    17701 agaccggcag cgtatttctc cagggcgtcg gcaaactgag ctgctctgtc cggctgtagt
    17761 gcgttatcca gcgcctgcgc cgagttccag gtggcatccc aggcgtgaag aatatcgcgg
    17821 ccctgacgaa taaccgtttg cgaacgttca atcagtaagg cgcgttcttc gctgtccagt
    17881 tgacgctcca gcgccacgcg ttggcacatc tcttcacagg cattaaacgc cgcatgcaga
    17941 ccgatacgaa tttgcagata agtgcgcaga gccgtgactt cctgctggcg tgggatccgc
    18001 attactttac ttaacatacc cagcgtgcca gccagttttt gcggcagtgt gcgcgcttca
    18061 ctttcaggcc agacaaaggt gtaaatcacc gcggacacca cggtaccaat gatgataccc
    18121 agggcgcgat cgcgaatttc caccaggtcg tacactgggc caaaaacgtt ttcgagcgtg
    18181 gcgagcgcga aggtgaccac catctgtgtg ccgatataag aagagcgttc agagctggtg
    18241 gcaatccatg cgcccaacag gaaaatcggt gccagcacaa acagtaattc gacaatattg
    18301 tccagccagg gcatgaccag tagcgtgaat aacagcgcca gaatcgcgcc gcaaaaggcc
    18361 ccgccaaaac gcagcaccat cttctggtac gacgaaccga catttgggtt agcgacgatc
    18421 acgcatgtca gcatacaggt gtgaatgcct tcccagtcca cgccgctgta aaaggtgtaa
    18481 cagatcaaac aggcgagcag cgtttttacc gcgtagcgca tatagtctgg attggtaaaa
    18541 gcatcggcgg ccattgatgg cggtttggct gcgggcgttg gcggcgtatt cgggtccatc
    18601 tgacccagtt gtaacaacgt ctggcagata ttctccaggt tacattcccg tgccgccatc
    18661 gcttcacttt cactgatccg ccagtcgctt tgccagcact gaccttcagc aacggcatgc
    18721 tgcagcttat tgatttctga ggctaatttt tgccgaaatt caataattgc ctgagaatca
    18781 gcaaaagagg tgggatcgta gcgattcagc gtcgagtaaa tgtaggttac cgttgccacg
    18841 cagctttgcc accatgcgct ttgagttcgc cagttggcat cgtccgcgag acaaaagaca
    18901 ttcagttttt gcagcgccag cgcctctctt tcaatccgcg tttcgggtag cggtgcgagg
    18961 ctgtccgtca ggtggctaat ggcatcatca agccgatcat taagcgcctg atgcatttgc
    19021 gaaatggcac gactgggaaa ccacagcacg ccgattaacg tcatcagcaa ggttggataa
    19081 aggccaacaa cgatacacca cagcgttaag cgcacgacca cttccggata gtcgagcatg
    19141 gcggggaagg tttgcccgta aatggcgaca atggcgactg cgaaaaagac cagtcccagc
    19201 cgatgggtgc gcatcaaaaa catacagccc atcaggatcg gcccggcgat gaacaatcgg
    19261 atcaacggtt cgccgtatga ccatttgtag atcaaaaaca ggctgccgat ctccagcatc
    19321 gtggcgacca caaataagat cgcgacaaat ttggtgtaaa acgcgttcga ctgaataccg
    19381 taaaacagca ctgccagcga taacgccacg aaagggatct caaaggtcat cgagatcaga
    19441 atcaccagca ggcagcctac ccagagttgc acggtctgcg gcacgcggcc tggtcgccgc
    19501 tcgcttaact cttcatgaaa gaacgccagc agcctgacca ccggtaatgg cagggagttg
    19561 agcgcgctca ttattgcggc tcaagattag cgactgccga agcgccgatg cggaacattt
    19621 ccgggtcagg tttatcgacc atgattttga ccggaaaacg ctgggcaacg cggacccagt
    19681 taatagagcg agaaactttc ggcaggccgc ccagcaccag gccgccgtca tccggtagca
    19741 cgccgtagcc aatcgaatcc actttaccct cgaaggtttt gccgctgtca ctcatcaggc
    19801 gaatcgttgc gggtgtacct gagcgaatat ttttcagatc ggtttcgcgg aagttggcga
    19861 tcacgtacca gtgacgagtg tcgattaggg taaaaatagg gcgcatggca gaagcaaatt
    19921 gcccgacgga ggttttgagg gaaatgaccc ggccatcaaa cggcgcgcga acggtcgcca
    19981 tttccagatg cagtttggtc agggcaatat ccgcttcgac cgccgcacgc tgggcaacta
    20041 atgcatccac gccgctgacg gcgctggcgg ctgactgcgc ctgcaacaat acagcattaa
    20101 gatcggcttc tgcagcgcgc tgcgcggtcc tcgcacggtc aacgtcttcc gctgagacaa
    20161 aaccttcttt cagtaatggc tcggtgcggc gtaatgtatc tgtggcctgt ttcgcggcgg
    20221 cacgggcttt ttctaccgtg gcgttaaccg agtcggcacc aaactgttgc gcgtcaacgc
    20281 tacgctgggt gagcataatt tgcttatcca gcgccgcgag ggaggcttca gctttcgcca
    20341 gattggcttc gtacgggcgc gggtcaatgc ggaacagcaa atcgccctgt ttgactgcct
    20401 ggttgtcggt gaccgccagt tctacaatgc gaccgctgac ttccggcacc acatcaatgg
    20461 tatctgctga cgcgtaagcg tcattagttg atggcgcact gtctacgcgc cagataacga
    20521 aaacaagggc aaccagcgcc aacgcaacca ctaacagagc agggaattta ctgcgaggag
    20581 cttttttcgg cgtactttcc atgagcgcgt ctcaaataga ttagaaaaat gccagccaga
    20641 acagcatggc gtagagagca aaaagggcgg tataaataat tccgacaaat gccagattga
    20701 tatttgcccg ctgaataact cgcctcgtga tgagcatcaa aataaggctg gcaatggcgc
    20761 agaaaaacca gctgggataa taagcaccga tcaccgggat tgccggagat aatgagcatc
    20821 ccgaaaccat gaagacggga attatccagg ctgttttttt gagttgcatc gccatgcgag
    20881 agagaactgt tggcatatta aatacatcct gtctgctgcg aacaatatct gtagtgaata
    20941 tcaatatcca ttgaaggcgt cagtatggct attttctcga tgaataaaaa tagctttatt
    21001 cagccaccgc attcataact tatatatttt atttattaat tggctgtata tatt
//
LOCUS       DAZGJW010000078        20904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZGJW010000078 DAZGJW010000000
VERSION     DAZGJW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.078"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..538)
                     /gene="mocA"
                     /locus_tag="ACHKIK_002929"
     CDS             complement(<1..538)
                     /gene="mocA"
                     /locus_tag="ACHKIK_002929"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO9084067.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTP"
     gene            641..1411
                     /gene="yqeC"
                     /locus_tag="ACHKIK_002930"
     CDS             641..1411
                     /gene="yqeC"
                     /locus_tag="ACHKIK_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO9084068.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1459..3084
                     /gene="yqeB"
                     /locus_tag="ACHKIK_002931"
     CDS             1459..3084
                     /gene="yqeB"
                     /locus_tag="ACHKIK_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO9084069.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSTA
                     EAMKLTERGFIPVMADPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3125..4057)
                     /gene="arcC"
                     /locus_tag="ACHKIK_002932"
     CDS             complement(3125..4057)
                     /gene="arcC"
                     /locus_tag="ACHKIK_002932"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO9084070.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALLDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4105..5490)
                     /gene="hyuA"
                     /locus_tag="ACHKIK_002933"
     CDS             complement(4105..5490)
                     /gene="hyuA"
                     /locus_tag="ACHKIK_002933"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO9084071.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5543..6754)
                     /gene="ygeY"
                     /locus_tag="ACHKIK_002934"
     CDS             complement(5543..6754)
                     /gene="ygeY"
                     /locus_tag="ACHKIK_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO9084072.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(6812..8008)
                     /gene="dpaL"
                     /locus_tag="ACHKIK_002935"
     CDS             complement(6812..8008)
                     /gene="dpaL"
                     /locus_tag="ACHKIK_002935"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO9084073.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8066..9253)
                     /gene="ygeW"
                     /locus_tag="ACHKIK_002936"
     CDS             complement(8066..9253)
                     /gene="ygeW"
                     /locus_tag="ACHKIK_002936"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO9084074.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            9732..11510
                     /gene="ygeV"
                     /locus_tag="ACHKIK_002937"
     CDS             9732..11510
                     /gene="ygeV"
                     /locus_tag="ACHKIK_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO9084075.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(11550..12029)
                     /gene="xdhC"
                     /locus_tag="ACHKIK_002938"
     CDS             complement(11550..12029)
                     /gene="xdhC"
                     /locus_tag="ACHKIK_002938"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO9084076.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(12026..12904)
                     /gene="xdhB"
                     /locus_tag="ACHKIK_002939"
     CDS             complement(12026..12904)
                     /gene="xdhB"
                     /locus_tag="ACHKIK_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO9084077.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(12915..15212)
                     /gene="xdhA"
                     /locus_tag="ACHKIK_002940"
     CDS             complement(12915..15212)
                     /gene="xdhA"
                     /locus_tag="ACHKIK_002940"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO9084078.1"
                     /translation="MEAREATATGESCMRVNAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            15627..16382
                     /gene="actS"
                     /locus_tag="ACHKIK_002941"
     CDS             15627..16382
                     /gene="actS"
                     /locus_tag="ACHKIK_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO9084079.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            16461..16534
                     /locus_tag="ACHKIK_002942"
     tRNA            16461..16534
                     /locus_tag="ACHKIK_002942"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16493..16495,aa:Gly,seq:ccc)
     gene            16669..18276
                     /locus_tag="ACHKIK_002943"
     CDS             16669..18276
                     /locus_tag="ACHKIK_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9084080.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPSASYEELREHLKTIVEWELGVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLKGDNSGLLSYLE
                     PETGSLRPSVSLSVSSEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTAGKPYHYYVTALAAITGARLNEVAQLQVKDVRATEAGTVYIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTAGDNAMVFDGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVDRLVNVMAGVYKETGLNG"
     gene            18269..18928
                     /locus_tag="ACHKIK_002944"
     CDS             18269..18928
                     /locus_tag="ACHKIK_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFO9084081.1"
                     /translation="MAEYYPAVFEAEAFNCPHCGVYARQFWRRMYGSARELAQRTTEF
                     RMSTCSHCGDDAYWYDGNMIIPAAGNVELPNPDMPDNCKSDYMEARSIINLSPKGAAA
                     LLRLCLQKLMVHLGEPGENINKDIRSLVQKGLPVRIQQAADICRIVGNQAVHPGEISL
                     DDDPQLAHGLFKLLNIIVDDRITRPKEIEAMFQSMPEGPRQGIENQDRQAREQQQAAN
                     E"
     gene            19837..20565
                     /locus_tag="ACHKIK_002945"
     CDS             19837..20565
                     /locus_tag="ACHKIK_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO9084082.1"
                     /translation="MSQQEISVINLDQLVSMTSVEIAELTGKEHRNVLRDIRNMAEEL
                     NALKTEQCSKLSSHIGVTEDVYLNAQGKQQPLYRLDRKHTFILVAGYSVHLRAKCYDH
                     IQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKN
                     PISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPAIVDKLRGILIA
                     FTGTDQQKQEHLLKQAQSGKLEGL"
     gene            20567..20767
                     /locus_tag="ACHKIK_002946"
     CDS             20567..20767
                     /locus_tag="ACHKIK_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084083.1"
                     /translation="MIDPRTPIGRATLRYRGLPTRHLLSLLRLGVDNPDRPYYSRDEL
                     IAMLVDRDLNNQLRRAFAKLES"
BASE COUNT         5108 a         5106 c         5380 g         5310 t
ORIGIN      
        1 ccggagtatc aatatctaaa attatttctg cattttctat ctcgacggaa taatgagctc
       61 ccataaggag tgcctgacgc atattggtga cattgggtcg ttggattgcc tgcatcaagc
      121 atggttttga cactaaaatc ggatggccgg ggatgccatt atggagtggc agtattgcgc
      181 catcatttcg taacgaccag atttttctaa aaatatcgat ggtgagggtt ggcatgtcac
      241 cgtgggtgag aaaacaatgt tctgtttgca ctgcgggtac tgcggccttt actgatgtta
      301 gtaaaccctg cgcataatct gggttgtgga taatagtgat attgctctgg ttcgcataac
      361 gttcgtgcag ttcattacca cgatagccgg tgactaaaat aattcggcta caaaactgca
      421 acgcattttt gatacttgta tcaagaattg tcccctgttg ccagggtaac atcattttcc
      481 attgccccat tcttgatgat aatccggcag cggtaattat acagtcgatg gctgacatag
      541 tggttccatt atttataggg gctaataacg cctgataatt gcgttacttc ctgatggatt
      601 aagttgggga taatttatcc cagagaggtc ataaagactc gtgaaaagta tagttgaccc
      661 atcggcatta gtcattgatt taggtgcgca gaaacgtccc actgtgattt ctgttgttgg
      721 tgctgggggg aaaaccagct tgcttttttg gctggcagaa ctgctccagg ctagcggcag
      781 gcgtgtgtta atcacgacga cgacacatat gtttatgcca acgtctcact ggcccgtggt
      841 tttctgtcgt gatcccgcca tgcttcctca tgcgtctctt acatccccca tttcattttg
      901 ttttcacagc tggaaagcga accagggaaa agtgcaggga tttacgccag aagcgattga
      961 tgcactggtg caacgaccag agtgtgacgt aattctcatt gaagcagatg gctcgcgtgg
     1021 aatgccgtta aaagcgcctg atgagcacga accttgcata cccaaaagca gttgctgcgt
     1081 gattgctgtg atgggaggac atactttggg cgcgaaagtg agcacagaaa atgtccatcg
     1141 ctggtcgcag tttgctgata ttaccgggtt aacacctgat gcaaccttgc aactgagcga
     1201 tctcgttgcg ctggttcgcc accctcaggg ggcgtttaaa aacgtaccgc aaggttgtcg
     1261 gcgagtctgg ttcattaacc gtttttctca atgtgagaat gcgattgcac aaagcgagct
     1321 ccttcaaccg ctgcaacaac acgacgtaga ggcaatctgg ctgggcgata tacaagaaca
     1381 tcctgcaatc gcgcgcagat ttgtgaatta gcggcccggg tattccgggt tactgaaagg
     1441 atatccattg aggttggtat gaatattttc acagaggctg caaaactcga agagcaaaat
     1501 tgtccgtttg cgatggcgca aattgttgat agccgaggct cgactccccg ccattctgca
     1561 caaatgttag tgcgcgccga tggttctatc gtcggtacaa ttggtggcgg aatggttgag
     1621 cggaaggtga ttgaagagtc acttcaggca ttgcaggaac gtaagccgcg attattccat
     1681 ggacgtatgg ctcgtaacgg tgcggatgct gtcgggtcag attgtggagg tgcaatgtca
     1741 gtatttatca gcgtccatgg tatgcgtcca cgtctggtgt tgattggcgc ggggcatgtc
     1801 aaccgggcga tagcccagag tgcggcgcta ttaggatttg atattgccgt tgccgatatt
     1861 tatcgcgaaa gcctcaatcc tgaactattc cctccatcaa ccacgcttct ccatgctgag
     1921 tcgtttggtg cagcagtgga agcactggat attcgccctg ataattttgt cctgattgcc
     1981 acgaataatc aggatcgtga agccctcgac aaactcattg aacagcccat tgcatggttg
     2041 gggttgctgg caagtcgccg caaggttcag cttttcctgc gtcaattgcg tgagaaaggc
     2101 gtggctgaag aacatattgc ccgtttacat gcgcccgttg gttacaacat aggtgcggaa
     2161 acgccgcagg agatcgccat cagcgtgctg gcagaaatat tacaggtgaa aaataacgcg
     2221 ccgggtgggc tgatgatgaa accttctcat ccttccggac accagctggt ggtgattcgc
     2281 ggtgcgggag atatcgccag tggtgtggcg ctacgtctgt atcatgcggg ttttaaagtg
     2341 atcatgctgg aagtggaaaa accgacagtg attcgttgta ccgtggcgtt tgcccaggcc
     2401 gtgttcgatg gcgaaatgac ggtcgaaggc gttactgctc gcctggcaac cagtactgcg
     2461 gaagcgatga aacttaccga acgcggattc atccctgtga tggcagatcc cgcctgttca
     2521 ttgcttgatg aactgaaacc gctttgcgtg gtggacgcta ttctggcgaa acagaatttg
     2581 ggaacgcggg cagatatggc accagtaaca atcgcgcttg ggccgggctt tactgcaggg
     2641 aaggattgtc atgcggtaat tgaaacaaat cgcgggcact ggctcggtca ggtgatttac
     2701 tctggttgtg cgcaggagaa taccggtgtt cctggcaata ttatggggca taccacccga
     2761 cgggtgatcc gtgctcctgc tgcaggcatt atgcgatcca acgtgaaatt aggcgatctg
     2821 gtgaaagagg gcgatgtgat tgcctggatt ggtgagcatg aaattaaagc accgttgacg
     2881 gggatggtgc gtggcttgtt gaacgacggc ctggcagtgg tcggtggttt taaaattggt
     2941 gatatcgatc ctcgtggtga aacggctgat ttcaccagcg tttctgataa agcccgggcg
     3001 attggcggcg gcgtacttga ggcgttaatg atgttgatgc atcagggcgt gaaagcgaca
     3061 aaagaagtgc tggaagtggc ttaaatgaaa atggccggat ggtattaccc acccggcctt
     3121 aaaattactg acaaatcacc gttccggttt tgccttcaat cccctctttc gctttgctca
     3181 atacggtaat cagtgcttca cgacctgcgc gagagcgagc aaacgaggcg gcagcttcca
     3241 ctttaggcag catagaacct ttcgcaaaat gaccttcttc gataaagcgt tcagcatcac
     3301 ttaatgacag gcgatcgagc cactgttcat tctctttacc gaagttaatg gccacttttt
     3361 ctaccgcagt cagaatgatc agcatatcgg catcaatcat ttctgctaaa cgggcgctgg
     3421 cccagtcttt atcgataacc gcgctggcac cacgcagatg gttaccttca cgaataacag
     3481 gaataccacc accgccaacg gtgatcacca cctggcctgc atctagcaga gctttaaccg
     3541 tttctttttc aataatatca accggttttg gcgaggcaac aacacggcga tagccgcgac
     3601 cagcatcttc tttcagagtg taaccctgtt ttgtcagttg ctctgcttct tgctcggtaa
     3661 agaacgaacc gatcggcttg gtcgggttga ggaatgccgg atcgttagcg tcgacttcga
     3721 cctgagtaac cagcgttgct accggtttat taatgccccg agaaagcagt tcttctctta
     3781 aggcgttttg tagatcgtaa ccaatataac cctggcttaa ggcaacgcag acagacatcg
     3841 gcagcattgg cgagtgcgct tcagtttttg cagcagcttc aaaggcctgg ttaatcatgc
     3901 ccacctgcgg cccgttacca tgagtgacga taacttcatg tccttgggca attaaatcaa
     3961 caattgcctg agaagtaatt tttaccgctt tcatttgtcc ggccaggtca tcgcccagcg
     4021 cattcccgcc cagggcgaga acaattttct tactcatttt tcctcactca tcattcttga
     4081 ttgtgtatag atccctgaca gtggttagag cacgggaggg acaaacggtt tgcgtcgcag
     4141 gaaacgacct cgcccggctt tgcctgtaaa ggtgccgtca cagaaaatcg tttcaccacg
     4201 ggataaggtt ctgacaatcg cgccctgaca ggtaaaaccc tcccatggcg agtagtcggc
     4261 gttgtcgtgg agatggcgat gctgaatttg ttggctctga cgtgggtcga taatcaccac
     4321 gtcgccatcg gaaccgggcg ctaataatcc tttttgcggc cacaggccaa acagcctggc
     4381 gggcattgcg ctggttaatt caacaaagcg ttccggtgtt atacgtcccg tcatcacgcc
     4441 actggaaaac agtaactgca tgcggttttc cacaccgggt aagccatttg ggcagcgact
     4501 gaaatcgcct ttagaaattt gcaggcgttg agccatcgag aaggtgcagt gatcggtcgc
     4561 caccacgtca atcgcaccat cgctgatgcc acaccacagt ttgtcctgct cgcgtacgtt
     4621 acgcagcggt gggctaagaa tgaacttcat gccgtcttct gtatcgtaac tgcgttcgtc
     4681 caacaggaga tattgtgggc aggtttcaac ccagactggc tggtgattcg cacgggcaag
     4741 acgcagataa tccagaccta agccgttaga caggtgcacg atatagagcg gggcgttacc
     4801 ggcaatttgt gccaggttaa tcatgcgggc gatggcttcc gcttcgcatt ccagagggcg
     4861 actcaaggca tgatagcgcg gcgcggttaa ccctgcggcg ataaattccg cccgcttgct
     4921 ggcgatagcc gcatcatttt ccgggtgcac ggtggtcagc gcgccggatt catgcagacg
     4981 gcgtaatgcc tgcaaaacct cgtcatcgtt gagtttgtat tgataggtta aatagagttt
     5041 aaaactgctc agtccttcct cgaccatcat cgggatttcg tcgaggattg cgtgattaat
     5101 gtgctggatc acaccgtgaa agctgtaatc gatgaccgct ttatgggcgg cataaccacg
     5161 ataaacctcc agttgatggc gtaaccgaca gccgtttggg ccaaatccca tatggtcaat
     5221 aatggttgtt gtaccgccac acgcagctgc gcgggtaccg gtaaaaaaat catcacaact
     5281 gcgcgcgatg ccgacatcaa tattgaaatg cgtatggaca tccacgccgc cagggaaaac
     5341 gtaacagcca gtggcatcaa tttcttcata cgggagctgc ggcgaaatat tgttgcccaa
     5401 ctggcgaaca atcccgcttt caatcagcaa atcctgtttg gcttgtccat ctgcgttaac
     5461 gacagtgccg tttttgatca atacgcgcat agcaaactcc aaaaaccctc cggcgaaccg
     5521 gaggggaata agtgaagagt aattactctg ttgccagcca gcttaacggg attgcagcgt
     5581 acatcgcggc acaggtcacc aggtgagatt tccaggtttt ttcgttaggt gcatgcgctt
     5641 caggttcttt acccgggcca aagccgatca ccggaatgcc gtgacggccc atgatagata
     5701 cgccgttagt tgagaaggtc cacttatcaa caaccggcgc tttgccaaac agcccttcgt
     5761 aggcattcac cagtgcttta acggtgaagt gatcttcttc cactttccag gtcgggaagt
     5821 agcattcggt tgggtaaacc aggccagtcc aggacggacg gtcgtagttg tacatagaaa
     5881 caaccgcgtt agctttctgt actgcaggca gggcgcggat ttcgtccagc gcgccttccc
     5941 aggtttcgcc ccaggtcaga cggcggtcaa tagagacggc gcagctatct gctacagcgc
     6001 aacggcttgg ggatgtgaag aagatttcag aaacggtgag ggtgcctttg ccgaggaatt
     6061 cgtcataacc cagacgttgg gagagttctt gtaattcgcc aagaatcgga cccattttga
     6121 aaatggcgtt gtcaccgcgt tctggcgcag aaccgtggca gctaacaccc tgaacatcaa
     6181 tacgaatttc catacgaccg cgctgaccac ggtatatctg gcagtcggtt ggttcggtac
     6241 tgaccacaaa ttccgggcga atgccggatt gttcaataat gtactgccag cacagaccgt
     6301 cgcagtcttc ttcctgcaca gtaccggtaa ccagcagggt atattcatct tccagaccga
     6361 ggtctttaat gattttaccg gcataaacca tagatgccat gccgccttcc tggtcggaag
     6421 taccgcgacc accgattagc tcatcagttt ccatgccttc gtacggatcg aagtcccagt
     6481 ttttgatgtt gccaatgccg acggtatcga tatgagcgtc cattgccacc agacgcgggc
     6541 cgtggccgat ataaccgaga acgttgccca tcgggtcgat ttcaacttta tcgaagccga
     6601 ctttttccat ctcttcttta atacgatgta ctacgcgttt ttcgtcgcag ctttcactgg
     6661 gaatagcaac catgtcgcgc aggaagcgag tcatgtccgc ctggtaatct tttgcttttt
     6721 caagaatcag tttgaatgga atattcttag ccattatttt tctccagatt ttttactccc
     6781 cgttttgcag ggagtagttc caatagagtg attaaggtgc tacagcgtgt ttgccttccc
     6841 agacaacttc gcggtagtgc ttcacgtcgg tgtcgccttc agtgctgata accagcacca
     6901 cggcatcttt gttcagcgcc agtttttcca tcaggctttg acgttgcggg tgataatgaa
     6961 ccgctgcgag aacgcccaaa ccgacagcgc cggattcacc ggagatgatg cgcgggtcgt
     7021 tgccgtacgg attacccagc acgcgcatac ctaatgcggc aacgctgtcc tggcaggaga
     7081 tgaattgggt ggcgcagtta cgtaggattt cccagcccag cgggttaggt tcgccacagg
     7141 ccaggcctgc catgatggtg gccatatcac cgccaacgtt gacgatgtcg cctttgacgc
     7201 cggagcgata aatacagtca gctttgtcag gttcaacaat aatgctgtgc agattttgcg
     7261 ggctatagac gtcgaccaga taacccagca caccaccggc cattgctccg acaccggctt
     7321 gcagcagaac gtgcgtcggg gttacgccca tttcacgcat ttgctcgacg gcttcatctg
     7381 ccagggttgc gtagccttgc atgatccagg ttgggatttt ggtgtaacct tcccatgccg
     7441 tgtcctgtac cacttcccag ccgtgctgct gcgcgtgttg catggtcagg cgaacggtat
     7501 cgtcatagtt catatctgtg acgatgcact cggcaccgag gttcagaatg gcgtcaacgc
     7561 gttcctgagc agaacctttc ggcatgtaaa tcaccgcatt ctgtccgagt tgctgcgctg
     7621 cccacgccac accgcgcccg tggttgccgt cggtggtggt cgcgaaagtc attttttcgc
     7681 cgatggcatt tttcaggtgc tcaaatgaca gcgtttcgat atcaagatga tatttttcac
     7741 acaataattg agcgatagcg tacgcaccgc cgagcatttt gaaggcgttc agaccgaatc
     7801 gttttgattc gtctttgaca agaattttct tcaccccaaa aaggtttgcg agatcgtcca
     7861 gcgcacaaag cggtgttggg cgataaccag ctattttctg gtggaactgg cgcgccagtt
     7921 tggcctggct ttgcgaaaag agcggtgatg tttcgccgtt gaaaaatttg ttatcggcga
     7981 tatcaatctt caatgagaaa acggacatat cctatccttt tggcctctgg aaatagaggg
     8041 ccgaccagct gatcggcccg aggggttatt tcacgcgttc ttgcgcttct ttcagcagtt
     8101 gctcgagcag tgcacctggt ttggcgtatt tacgggacag gatcatcgcg gcgatgatat
     8161 aaggcttcca gctggcttct ttgtaggtag cgatacggta tttttcgaat acgccttcag
     8221 tcacttcccc ttctttgcag gatacgccgc tgatatcagc tggcaggcag tgcatgtaca
     8281 gggcttcgcc atcacgggtc agttccatca tctcttcagt acaatgccag tctttgtgtt
     8341 gcgcgttctg tgccagacac tgtttttcca gtgcttttaa gccttcgtga tcgttagcgc
     8401 gcagcaattc ggtacgctgt tccatcactt tgtaaggtgc ccatgacttc ggataaacga
     8461 tgtctgcatc tttgaacgct tcttccatgc tggtgacctg acggaagcta ccgccagatg
     8521 ctttagcatt gtttttcgcc acttcaacca catccgggat caggtcgtag ccttccggat
     8581 gggccagggt gacatccata ccgaagcgag tcatcagacc gatgatgcct tgtggtacag
     8641 agagcggttt gccatagctt ggagagtagg cccaggtcat ggcgattttt ttacctttca
     8701 ggttttccag tgaaccaaag tgttcacgta accacgcgag gtcagccatt gactgagtcg
     8761 ggtggtcaat atcgcattgc aggttcacta aagccggacg ctgtggcagt acaccctgct
     8821 tgtaaccgtc atcaagtgca gcgccaactt cacgcatata ggcgttgcct gcgcccagat
     8881 acatatcgtc gcgaatacca atagcgtcgg cgcagaagga gatcatattg gcggtttcac
     8941 gcacggtttc gccgtgagcg atttgtgatt tgccttcgtc gagatcttgt tgtgcaaggc
     9001 cgagcaggtt aagcgcggaa gcataagaga agcgggtacg ggtggagttg tcgcggaata
     9061 cggaaatacc taatccacta ttaaagactt tggttgagat gttttcagca cgcagtgctt
     9121 ttaatgctgc ggcaacgtcc agtacttgtt tcagttcatc tggcgtctgt tcccacgtta
     9181 acaaaaaatc tttctcgtga aggtgagagg tcagcgaatt gatatcctta atcagctcat
     9241 taacagtttt catcataaag tcccggtagt ggaatgacag gcaaagtggt ttatttcact
     9301 gtgacttgcc aaagtgattg gtgttttcac agctatacct gggactaaca ataagtgtgc
     9361 cagaattatc cacggggctg caaaagacta atttgacgga taatgtgtgg ggttgatcac
     9421 aaattgatgc cagcttccgg ttgtttgtaa aatacgaaaa cgtttggctt tttttatcgc
     9481 gttatttatt tgctgctaag tatcagaaat aagataaagg gacaggctat ctctcttcag
     9541 tttgataaac tcaccgccgt aattctcata ttgatataga aatgtgatat ctcttccata
     9601 atttcattag aaaatgataa aaatcttatg tttttatcaa agccaatttc gcctgagtta
     9661 atatcatcat gatctgtgaa ccatcaacgt cttcaatgat cgacttttaa gtaagtctgg
     9721 aaggtaaagc tatggagctt gctactacgc agtcagtatt gatgcaaatt caaccgacaa
     9781 ttcagcgttt tgccagaatg cttgccagcg ttttgcagct tgaggttgag atcgttgatg
     9841 aaaacttgtg tcgcgtcgcc ggaacgggcg cgtatgggaa gtttcttggt cgccagttga
     9901 gcggcaactc acgcctgctc cgccacgtcc tggaaacgaa aactgaaaaa gttgtgacac
     9961 agtctcgctt cgatcccctt tgcgaaggtt gcgatagtaa agaaaattgc cgtgaaaaag
    10021 catttctggg tacgcctgtc attttacagg atcgttgtgt tggggtgata agtttgattg
    10081 ccgttaccca cgagcaacaa gagcatatca gtgataattt acgcgaattt tctgattatg
    10141 ttcgccatat atccaccatt tttgtttcga aacttctgga ggatcagggg ccaggagata
    10201 acatcagtaa aatattcgcg accatgatcg ataatatgga tcagggcgta ttagttgttg
    10261 atgatgaaag tcgggttcag tttgttaatc agactgcctt aaaaacactt ggtgttgtac
    10321 aaaataatat tattgggaaa cctatccgtt tcagaccatt aacatttgag agtaatttta
    10381 ctcatggaca tatgcagcat attgtttcgt gggacgataa aagtgaatta atcattggtc
    10441 aattgcataa cattcagggc cgacaattat ttttaatggc atttcaccaa tcgcatacca
    10501 gtttttctgt agcaaatgca cctgatgaac cacatattga acaattggtt ggcgagtgcc
    10561 gtgttatgcg gcaattaaaa cgactcatta gccgtattgc acccagccca tccagcgtta
    10621 tggtggttgg tgaaagcggc acgggtaaag aagtcgtcgc ccgagcaatc cataagttga
    10681 gcggaagacg gaataaaccc tttattgcta tcaactgtgc cgcgattccg gagcagcttc
    10741 tggaaagcga actgttcggt tatgttaaag gcgcatttac tggcgcttct gccaacggta
    10801 aaacagggtt gattcaggcg gcgaatacgg gcacgctgtt tctcgatgaa ataggtgata
    10861 tgccattaat gttgcaggct aaattactgc gcgctattga ggcgcgtgaa attctaccga
    10921 ttggtgccag tagcccaata caagtcgaca ttcgcatcat ttctgcaact aatcagaatt
    10981 tggcccagtt cattgccgaa ggtaaattcc gcgaagatct cttctaccga cttaatgtta
    11041 tcccgataac tctgccaccg ctgcgtgaac gtcaggaaga tattgaacta ttggtgcatt
    11101 actttttaca tctgcatacc cgtcgtctgg gatcggttta tcctggcatt gctcccgatg
    11161 tcgtcgaaat attgcgtaag catcgttggc ccggaaacct gcgcgagtta agcaatttga
    11221 tggaatatct ggttaacgta gttccttcag gtgaagttat cgacagcacg ctattgccgc
    11281 caaatctgct gaataatggc acaacggagc aaagtgatgt aacagaggtc agtgaggcgc
    11341 acctgtcact cgatgatgcg ggcggcacgg cgctggagga gatggaaaag caaatgatcc
    11401 gcgaggcgct ttcacgtcat aacagcaaga agcaagttgc tgatgaactg ggcatcggca
    11461 ttgctacgct ctatcgcaag attaagaaat atgagttgtt aaacacataa cctcagtgaa
    11521 tccggcctga attcaggccg gatatccttt tacttcgttt tctcgcaatc cagaactgta
    11581 tttacaatca tctgataccc cgtgcagcga caaagatttc ccgccagtcc gcgacgaatt
    11641 tccgtaatgg ttaatggctt ctcgcgtggt ttcgccagca ttgccgtggt agccataatc
    11701 aggccaggcg tacaaaaccc gcactgcact gcgccggatt tcgcataagc ctgctgaaca
    11761 tgagaaagtt ttccgccttt cgcttcacct tccagcgtgc ggatctcttt tccttcagcc
    11821 caggcggcaa ggtataagca actgtctatt gctgtgccgt cgaccaacac cgtacaggca
    11881 ccacattcac ccacgcagca cccttgtttg acacttagca gtccttgttc gcggagtaat
    11941 tccgagagcg gcgtgcctgg tgcggcgtga agctgaaaag gcatcccgtt aatggtgcat
    12001 tcgatggtaa ttgtttcgct gtgattcatt gcaattttcc ccccgccgcg gcgacggctt
    12061 cgctaatcac ttttttggtc atcgtctgga tgagatgcag acgaaactct ttactggccc
    12121 gccatgaaga acgcggggcg acatcttgca ggacagattc gctgatagct tccagcgttt
    12181 gcaggtttaa tggcgcattt tgtgcagtct gttcggcatg ttggcagcga atcggcgttg
    12241 gcgcggcaac accaaatgcc aggcgtaatt cgctgaaatt gccgttatcc agtcggcaat
    12301 gtgcggcgca gccaatcgtt gaaatatcca ttgcgtcgcg catggcatat ttaaaatgcg
    12361 cgctgcccgc gtgttctttc ggctgtggcg gaaaatgaaa ggcgacgagg atttcgtcat
    12421 gctcaagaga cactttgccc ggcccggtgt gaaagccatt aatcgggacg aaacgaacac
    12481 cgcgcgggga gtggatctcc agtttcgcgt cataaattag cgttggcgtg gcagaatctg
    12541 cgctggtggc accgttgcaa atatttccac cgtaggtagc gacgttacgg atctgcggtc
    12601 cagcaatgga cgtggccgca gcacataacg ccgggagatg acgttgagtt ataggatctt
    12661 ctattagctg ggtaaatgtc gttgcagagc cgatacgtag cgagccatct tccgccagcg
    12721 taattccccg cagctccgcc agattatgaa tatcaacaat atggcgataa cggtcattgt
    12781 gatggtggag ctgaatcagt acgtcagtgc caccggcgag cagtttggcc tgcgggttgt
    12841 cagccagcag gttgatggca tcggcaaggg ttgctgcgcg atggtaagaa gcaaaatcaa
    12901 acatgatgtt atcctcaaat caatcctgcc agatggaact cttcatataa ccgttttggc
    12961 gtcagcggca gtgtattgat tgcaacaccg gtagccatct tcaccgcgtt acgaatagca
    13021 gcggcaacag gaattattgg tggctcaccc agtgacttat gtccgtatgc ggattgcggc
    13081 tcattgattt cgacgaacgc gctttccagt tgtggcagat ccggcatggt cggcattttg
    13141 taatccagca gattggggtt acggaccacg ccgcttttag catcgatgat catctcttca
    13201 aatagcgccc agccaatgcc cattcccatt ccgccgtgta cctgaccttc tgccagcagt
    13261 ggattaagaa tatgccctga atcatgaacg ttgaggatgc ggttgatggt gactttgcac
    13321 agcgcaatat cgaccgtcag atcaacaaag gtacagccaa acgccggtgg gttagtggtg
    13381 gttttgatgg agctttcagc agagagctgc ccgccgcgtt cagggtggta gaaagcgtcc
    13441 atcgccaaat cttttaacga cattaacggc tcttccggtc gttcaaccag cacgatatgg
    13501 ccttttatca gggtcagatt catcgctgac tgatgtagca tgactgcggc gtgagcgatg
    13561 attttctctt ttaataatag tgccgcactg cgcagcgcag gcgcggcaac atagctctgg
    13621 cgtgaggcaa atgcgccggg atcgaacggc gtaacgtcgg tatcttgagt tgaaataacg
    13681 cgaacgtcgc tgaccggaac ccccacggtt tctgccacca tttgcgagaa gacggtgtcg
    13741 gcaccctgac cgatttccgt cgcgccgctt tgcacgttga tggttccatc ctgattcatc
    13801 agaaggcgcg cgcctgctat ttctacgccg acaggccagg tgttagaggt gtagctaaaa
    13861 caggcgacgc caacgccgcg gcgcaaattg ccttgctggt tctggcattc tgcacggcgt
    13921 ttttcccatt caaagatttt ccggcctttt tcaagacact ccggcaaccc tgcgctgtaa
    13981 atacgtttgc ccgtgagcgg attagcatct ccttcgcggg cggcgttgcg taaacgaatt
    14041 tcaacaggat caatacctaa cgctgtcgcg gcgtcatcaa gcatagactc aacggcaaat
    14101 acgacttgtg gcgcgccata accacgcatc gcaccagccg aggggaggtt ggtatagcag
    14161 gtctttgaac tgtaagcgta ggcacaacga ggataaaggt aagcgacttt attccccccc
    14221 gcagaagcga tggagtgccc gtgagatgca taagcgccgg tgttagacag aacatccaga
    14281 ctataacctt tcaatgttcc gtcgcggttc acgcccattt gcccgtcaat ggtaaaagcg
    14341 tggcgggtac gggttgcgag gaaacactct tcacggctaa gggaaacttt caccggaatg
    14401 ccgccaagct tgctggtcag gaatgccgcc attggctctt ccagtacatc ctgtttatta
    14461 ccaaaaccgc caccgacaaa cggtttgatg actcgtacgc atgaccaggg aatatccagc
    14521 gcctgaccaa ccacgcggcg aacaatgtgc gggatctggg tgctggaaac gatggtaatt
    14581 cgcgagtcat cctccatcca cgccagcgat gttacgcttt ccatgtgaca atgttgaata
    14641 acgggggtct gatagtgccc ctgtacctgg tagtcggcgg catcgattgt ttgttggaca
    14701 ttacccgtcg acatcgtgct ttgtttcagt aaattgccac cgttatggat tggtgcagcg
    14761 tcttctgcca gcgccgcttc tggcgtggtg ataacgggta attcttgcca ctcaatgctg
    14821 accaattgcg ccgctttttc tgccgtgagt tcatcgcggg ccacgacgat ggcaacggcg
    14881 tcaccatgat gacgaacatg gcgagttagc agtgcgcgat cggcggtatc gcgcttgttt
    14941 tcgtcaagtg tccaggcatg cccagcagta gcaaatggaa tatcaggcac atcttcccag
    15001 gtaaaaatcg ccagtacgcc cggcaaactt ctggcttgtt catcattaat acttacggca
    15061 taaccatgtg cgatagggct acgtacatat ttcgcataac acatgcccgc cataacataa
    15121 tcgtcagtat atcgtgcccg cccggtgacc ttagcaatgg cattgacgcg catgcatgat
    15181 tcacccgtag cggttgcttc ccgcgcttcc atagaaatcc cctcgatttg aacatttggt
    15241 tcttatggca aagatttagt ctaagcaggc aagatttacg ccagaaacga cgataagcgt
    15301 taggggaggt aggaagtgtg atgacgttaa aaaaacacag atatgatacg aaacaggact
    15361 ctgaataggg gcagtgatac gtatcaatat gagtggcgat aaaatcaatg tgataaattg
    15421 ggattattac gcatcaggat gggaaatagg gtaaaaagtc aggaagttcg atacctctac
    15481 agcgaatatt ttgctgatga tacttccctt tgccaaatga aattaacctc tttctctatt
    15541 accacgtttt tccagaagca agagattggg tatcaagagg ctggctgcta tgataaggcg
    15601 tcttgttttt taagcgagga aagattttga gtgcgggacg cctgaataaa aaatctctgg
    15661 gtatcgtgat gttgttatcg gttggactgc ttttggcggg ctgttcgggt agcaaatcat
    15721 ccgatacagg aacgtattcc ggctccgttt acaccgtgaa acggggggat acgctatatc
    15781 gtatttcgcg caccacggga accagcgtaa aagaactggc gcgactgaac ggcatttccc
    15841 ccccttacac cattgaagtt ggtcagaaac taaaactggg tggggcgaaa agtagcagta
    15901 gtacacgtaa atcaaccgcc aaatcaacga ccaaaaccgc atcggttaca ccgtcatcag
    15961 cggtaccgaa atcttcctgg ccgccagtag ggcaacgttg ttggttatgg ccaacgacag
    16021 ggaaagttat catgccgtat tcgacagcag atggcggcaa taaagggatt gatatctcag
    16081 ctccacgggg tacacctatt tacgccgcgg gtgcaggaaa ggtggtgtat gtgggcaacc
    16141 agctgcgtgg ctacggtaat ctcatcatga ttaaacacag tgaagattac attacggctt
    16201 acgcccataa tgacacgatg ctggtaaata atgggcaaag cgtgaaggct gggcaaaaaa
    16261 tcgccactat ggggagcacg gatgcggcat ctgttcgcct gcatttccag attcgttacc
    16321 gtgcaacggc aattgatccg ctacgttact tgccgccgca gggcagcaag ccaaaatgct
    16381 gatggcgaat taatcagcag tcagcagcgc ggcacttgct aaggagagcg taaggtttat
    16441 aatgccttac gcatctcgaa gcgggcgtag ttcaatggta gaacaagagc ttcccaagct
    16501 ctatacgagg gttcgattcc cttcgcccgc tccatgacca ttcttaaagt ctactaactt
    16561 actgaatcct aagggatacg gcttaaagcc ttccgatagt tttactcact gtaaccaaca
    16621 ctgtgactaa ctttgttgaa gtagtgagca gacctaaggt ctcagtacat gctcaattca
    16681 agaacctacc tttatcagcg taacggtgtc ttttacatcc gtctcaggat gaagaccact
    16741 ggtcgcctga ccgcttcgct tccttctcac aaccggtata aactagcgtc agtatcacta
    16801 cggactaagg acagacgtac cgctatggct cactcacggc acatcaagtc agcacttaaa
    16861 gcaatacacg cagacaaccc tagcgcatca tatgaggaac tacgggagca tctgaagacc
    16921 attgttgagt gggaacttgg tgtcagccgt gatgacctga acgacccgga gtcctaccag
    16981 ctttacgttg accagtacga cgacatcaag tccaaccttc gggaagctgt agcgactgag
    17041 cgtctgactg tagaccagca tcgctatatc aatgacgtta tcggtgtact taaagcctgt
    17101 caggatagac tcaagggcga taactctggc ctgttgtctt accttgagcc tgagactggc
    17161 agtctaagac cgtccgtctc cctatctgta tcatcggagc ctgaagtccc tgaacctaaa
    17221 gcactgaccc tcgcgtctct cattgagcag tacgagcagg agaacgccca gaactggaag
    17281 ccagcgaccc taagcgaaaa ccgagcgtca cactctacgc taatcgagat attcgactat
    17341 ctggatattc aggatgtcgg caaggcgacc cgtgcagata tgcttagggt ccgtgaagtc
    17401 ctccaacagc taccgaagaa ccgcaagcaa cgctttaagt ctatgccgct gtctgacctg
    17461 ctgaaccggg agtctaagac tgactgtctt gatgtcgtta ccatcaacaa taaatatctt
    17521 atcaagatgg ctgctgtctt taagtgggcg gtacgtaacg acctgatagc taagaacctg
    17581 accgaaggtc ttgagctgaa agtcccgcag cgtaaagcct ccgacgctcg tgatgctttc
    17641 agtcctgagc aggtagggca actactggtc gccgctaaag cgtactctca gaagaccgca
    17701 ggtaagccat accattatta cgtcaccgct ctggctgcga ttacaggtgc gagacttaac
    17761 gaggtggccc agcttcaggt caaagacgtc agggctactg aggccgggac cgtatatatc
    17821 catatcaacg aagacgatag cagcctaccg ggtaaaagta ttaagaacgc tcatagcgac
    17881 cgctgtgtcc cgctggtcga tggcgcttat ggttttgtat tggctgactt tatgtcgctt
    17941 gtggaagacc gtagaaagac tgcgggtgat aatgctatgg tattcgatgg cctcaagctg
    18001 atgaagaatg gctacggtga gcaggtgagc aaatggttta acagaacgtt gctgcctaaa
    18061 gtccttgctg accgtagcgg cttagccttt cactcattca ggcacactgt tgcgacccag
    18121 ttaaaacaac atggggtcga gttagcgtat gctcaggcga ttatgggtca tagctcaggg
    18181 tctataactt acgaccgtta cgctaaagag gtcgaggtgg atagattggt taatgtgatg
    18241 gcaggtgttt ataaggagac tggattaaat ggctgagtat tatccggcgg tgtttgaagc
    18301 agaggctttt aactgtcctc actgtggtgt atatgcccgt cagttctggc gtaggatgta
    18361 tggaagtgcg cgtgagttgg cgcaaaggac aaccgagttc cgaatgtcaa catgctctca
    18421 ttgcggtgat gatgcttatt ggtatgacgg caatatgatt attccggcgg ctggcaatgt
    18481 tgaactacca aatccagata tgccagataa ctgcaagtcc gactatatgg aagcccgaag
    18541 catcattaat ctgtctccta aaggggccgc tgcactcctt cgtctttgcc ttcagaagct
    18601 aatggtccac ttaggcgagc cgggagaaaa cattaataaa gacatccggt cactcgtcca
    18661 gaaaggacta cctgtacgca tccagcaggc ggcagatatc tgtcgtattg tcggcaatca
    18721 ggcggtccat cccggagaga ttagtcttga tgatgacccg cagttggcac acggtctctt
    18781 taagctgctg aacattattg tcgatgaccg aatcacgaga ccgaaagaaa ttgaggctat
    18841 gttccagagt atgcctgaag gtccacgtca aggtattgag aatcaggaca gacaggcgag
    18901 ggagcagcag caggccgcta acgaatgaaa cccacactat aggggaaggg gggggactaa
    18961 actatcacta taggggagag accctaagtc cccccgggct taatacagcc accgacccac
    19021 acattcatta agtctcaggc ttccggtagc ggaccgattg gaaaccatcc gcaataagcc
    19081 tagtgttaaa tcaccttggc ctgagaccat tccctttaac gtagcaatct ctctcctttg
    19141 acaggcggag aacatcagac cgcagcggaa gtatccgtct atgcgaccat cggtgtgcga
    19201 gattgtgaag aaccaagaca tcattgctga ggtctgccat ccatttgtgg acaggcggaa
    19261 actcaaaaag gttagaccag tggtgtctga ggttggagct ttgagcctga ccagcaggct
    19321 tgaggagacc atgagttcat cttgttatgg attgtgttat cacaattcca ataagaagaa
    19381 ctttaagtcc agcctccagt cctgtcagcc cgaagtatac tacggtcgct atgatgtggt
    19441 tctccatact tcgggtctaa ctggaagtac tgaacacttt cgcgtcaggt tctccgaagt
    19501 tcattcctgt ttgactaaac tatcactata gggaccatat ctcgcctctg acctcctgag
    19561 agacggcaag cgagaaaccg tggtccacgc tagcttgcta gccaccagcc tctggtcagc
    19621 ctttgtgttc tttggtctcg tgacttaagt ctatcaccac acacgctcgg acatgctgtc
    19681 gaaccttcat ctctatggtc atcgcagacc actaacccca attacctcaa gtcattctgt
    19741 ttgcgtcctg ttgcctgctg gtctcccttt acgtgggggc ttgagtaggt cagtgggggt
    19801 gcgacttcag ggtgcaaaca aaaggagtac acgtaaatgt ctcaacaaga aatcagcgtc
    19861 attaacctcg accagcttgt ctccatgacc tcagttgaaa ttgctgagct gacaggcaag
    19921 gagcaccgaa atgtcctacg tgatattcgc aatatggctg aggagcttaa cgcgctcaaa
    19981 actgagcagt gctcaaaact gagcagtcat atcggggtga ctgaagatgt ctatctaaac
    20041 gcgcagggga aacagcagcc tctgtaccgt ctcgaccgta agcatacttt cattctggtc
    20101 gctggctatt ccgttcacct ccgcgccaag tgttacgacc atattcagac gttagagcgt
    20161 cgagtccttc aactggaaga ccagaagaag cgagcagcca ttcagtccgc taaccgccga
    20221 ggcgtgactt ggggcgacta ctgcaaaacc tatggtctac ccgctcagaa gctaatgacc
    20281 gcacttcttc agcatcgtgg tctctttcgt aagaacccaa tcagtaacga gtggtctgta
    20341 aatccaaagt attccgactg cttccgtatc attaagccgt cagaccagaa gttctcagca
    20401 ggtggttata actttcgctt caacgctaag ggattggagg tcttcggtaa gcctgcgata
    20461 gtcgataaac tgcgaggcat ccttattgcc ttcactggca ccgaccagca gaagcaagag
    20521 catctcctga agcaagccca atccggcaag ctggagggac tctaagatga ttgacccgcg
    20581 cacacctatt ggtcgtgcaa ctctccgcta ccgtggtctg cctacccgtc acctcctgtc
    20641 cctgctgaga cttggagtcg ataaccctga tcgcccttac tacagtcgag acgagctgat
    20701 agcgatgctg gtcgaccgtg atttgaataa tcagttacgt cgagcctttg cgaaactgga
    20761 gtcttaacat ctccggaaat ttccagacct taaccaacct tatttaaaac cttttgagat
    20821 gaccgatgcg gggagacccg taggcattac tgtgtctgcg ataccgatag tctcaactct
    20881 caagaggagt aattactatg tcta
//
LOCUS       DAZGJW010000079        20439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZGJW010000079 DAZGJW010000000
VERSION     DAZGJW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.079"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            264..359
                     /locus_tag="ACHKIK_002947"
                     /pseudo
     CDS             264..359
                     /locus_tag="ACHKIK_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            383..4897
                     /locus_tag="ACHKIK_002948"
     CDS             383..4897
                     /locus_tag="ACHKIK_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO9084084.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNIPVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            4898..6547
                     /gene="yfaQ"
                     /locus_tag="ACHKIK_002949"
     CDS             4898..6547
                     /gene="yfaQ"
                     /locus_tag="ACHKIK_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO9084085.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            6552..7328
                     /gene="yfaP"
                     /locus_tag="ACHKIK_002950"
     CDS             6552..7328
                     /gene="yfaP"
                     /locus_tag="ACHKIK_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO9084086.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            7603..10452
                     /gene="rcsC"
                     /locus_tag="ACHKIK_002951"
     CDS             7603..10452
                     /gene="rcsC"
                     /locus_tag="ACHKIK_002951"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO9084087.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(10538..11188)
                     /gene="rcsB"
                     /locus_tag="ACHKIK_002952"
     CDS             complement(10538..11188)
                     /gene="rcsB"
                     /locus_tag="ACHKIK_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO9084088.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(11205..13877)
                     /gene="rcsD"
                     /locus_tag="ACHKIK_002953"
     CDS             complement(11205..13877)
                     /gene="rcsD"
                     /locus_tag="ACHKIK_002953"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO9084089.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            14616..15707
                     /gene="ompC"
                     /locus_tag="ACHKIK_002954"
     CDS             14616..15707
                     /gene="ompC"
                     /locus_tag="ACHKIK_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO9084090.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGRGYDDEDILKYVDVGATYYFNKNMSTYVDYK
                     INLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            15819..16874
                     /gene="apbE"
                     /locus_tag="ACHKIK_002955"
     CDS             15819..16874
                     /gene="apbE"
                     /locus_tag="ACHKIK_002955"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO9084091.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQTAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            16948..18012
                     /gene="ada"
                     /locus_tag="ACHKIK_002956"
     CDS             16948..18012
                     /gene="ada"
                     /locus_tag="ACHKIK_002956"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO9084092.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADSEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANACEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            18012..18662
                     /gene="alkB"
                     /locus_tag="ACHKIK_002957"
     CDS             18012..18662
                     /gene="alkB"
                     /locus_tag="ACHKIK_002957"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO9084093.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            18738..20381
                     /gene="yojI"
                     /locus_tag="ACHKIK_002958"
     CDS             18738..20381
                     /gene="yojI"
                     /locus_tag="ACHKIK_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO9084094.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTNVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDRLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGQTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         4941 a         5015 c         5473 g         5010 t
ORIGIN      
        1 attcttaatg aaaatgagca taaaactaag acatataatg cttggcagct ttatgctctt
       61 ctgattggct gtatgtttga tgacaaaaaa ttagcgcaag aagaaaaaat taccttgcgc
      121 taatgctctg tctcaggtca ctaatcacac ctaactcatt gattaattgt gactctaaat
      181 gtagtgtttt gtcgcgtttt gcagtatgtt atttaatcta ttatatgaat aacataatga
      241 tttttaatgt atttttgtaa tttactctcc ttctgacatg gaaggacacc cgatggatac
      301 ccaacgattc caatcccaat ttcattggca tttatcgttt aaattttctg gcgcgatagc
      361 agcgtgcttg tccctgtctc ttgtgggaac agggcttgct aatgctgatg attcgcttcc
      421 ttccagtaac tatgcgccgc ccgccggggg aacattcttt ttgcttgctg acagcagttt
      481 tagcagcagt gaagaggcga aagtgcgact ggaagcgccg gggcgtgatt atcggcgcta
      541 tcagatggaa gagtacggcg gcgtggacgt tcgcctgtat cgtattcctg acccgatggc
      601 atttttgcgc cagcagaaaa acctgcatcg cattgtggtg caaccgcaat atctgggcga
      661 cgggctgaac aatacgctga cctggctgtg ggataactgg tacggcaaat ctcgccgcgt
      721 aatgcagcgt actttctctt ctcagtcacg gcagaatgtg actcaggcat tacccgaatt
      781 acatctcggc aatgccatta ttaaaccttc ccgttatgta cagaacaacc agttttcccc
      841 gctgaaaaaa tatcccttgg tggaacagtt ccgttatcca ctatggcagg ctaaaccggt
      901 cgagccgcag caaggggtaa aactggaagg cgcatccagc aatttcatct cgccgcagcc
      961 gggtaacatt tatattcctc tcggccaaca agagccggga ctgtacctcg tcgaggcgat
     1021 ggttggtggg tatcgggcga cgacggtggt gtttgtttcc gataccgtgg cgcttagcaa
     1081 agtgtcaggc aacgagcttc tggtgtggac cgcgggtaaa aaacagggtg aagcgaagcc
     1141 cggctcagag atcttgtgga ctgacggtct tggcgtgatg acccgcggtg tgaccgatga
     1201 cagcggtacc ttgcagttac aacatatatc gccagaacgt tcatacattc tgggtaagga
     1261 tgctgaaggc ggtgtttttg tctccgagaa cttcttctac gaaagcgaaa tctacaacac
     1321 ccgcttgtat atttttaccg atcgcccgct atatcgcgca ggcgatcgtg tcgatgttaa
     1381 agtgatgggc cgcgagttcc acgatccgtt gcattcatcc cccatcgtca gcgccccggc
     1441 gaagctttcg gtgctggacg ctaacggcag tctgttgcaa accgtcaatg tcacgctgga
     1501 tgcgcgcaat ggcgggcagg gaagtttccg cctgccagaa aatgccgtag ccggaggtta
     1561 tgagttacgt cttgcttacc gcaatcaggt ctatagcagc agttttcgcg tggcaaacta
     1621 catcaagcca catttcgaga ttggtttagc tctcgacaaa aaagagttca aaactggcga
     1681 agcggtcagc ggcaaactgc aactgcttta cccggatggt gagccggtaa aagatgcccg
     1741 cgtgcagtta agtttgcgcg ctcagcaatt atcaatggtc ggtaacgatt tgcgttatgc
     1801 cggacgtttt cccgtgtcgc tggaaggcag cgaaacggtg tccgacgcca gcggtcatgt
     1861 ggcgttaaat ctccccgccg ccgataaacc gagccgctat ttgttaaccg tctccgccag
     1921 tgacggcgcg gcgtatcgcg tcaccaccac caaagagatc ctcattgaac gcggcctggc
     1981 gcattactct ttaagtaccg ccgcacaata cagtaatagc ggcgagtcgg ttgtgttccg
     2041 ttatgccgcg ctggaatctt caaaacaggt tcctgttacg tatgaatggt tgcgtctcga
     2101 agaccgcacg agccatagcg gagagctacc gtcaggcggc aaatccttta ccgtcaattt
     2161 cgctaaacct ggcaactaca atctgacatt acgcgataaa gacggcttaa ttctcgctgg
     2221 gttaagtcat gccgtcagcg gtaagggcag cacggcgcat actggtacgg tagatatcgt
     2281 ggcggataaa acgctgtacc agccaggcga aactgcgaag atgctgatta cctttccgga
     2341 gccaattgat gaagcattat tgacgctgga acgcgatcgc gtggaacagc agtcgctgct
     2401 ttcgcatccg gcaaactggc tgacgttaca acgtttaaac gatacccagt atgaagcccg
     2461 ggttccagtg agcaattcct ttgcgcctaa catcactttt tcggtgctgt atacccgtaa
     2521 tggtcagtac agttttcaga acgccgggat caaagttgcc gttcctcagc ttgatatccg
     2581 ggtgaaaacg gacaaaaccc attaccagcc tggtgaactg gtcaatgtcg aattaacctc
     2641 gtcgctgaaa ggtaaacctg tttctgcgca gctaacggta ggcgtggtcg atgaaatgat
     2701 ctacgcgctg caaccagaaa tcgcgccgaa tatcggcaaa tttttctatc cgctggggcg
     2761 taacaatgtg cgtaccagct ccagtctgtc gtttatcagc tacgaccagg cgctctccag
     2821 cgagccggtt gcgcctggcg caactaaccg cagcgagcgg cgagtaaaaa tgcttgaacg
     2881 tccacggcgt gaagaggtgg ataccgcggc atggatgccg tcactcacaa ccgataaaca
     2941 aggcaaagcg tatttcacgt tcctgatgcc tgattcgtta acccgctggc gtatcaccgc
     3001 gcgtgggatg aacggcgacg ggctggtcgg gcaggggcgt gcttatctgc gttcggaaaa
     3061 aaatctctac atgaagtgga gtatgccaac ggtgtatcgc gtgggcgaca aaccggcggc
     3121 aggactgttt atcttcagtc agcaggataa cgaaccggtg gcgctggtga ctaaatttgc
     3181 aggcgctgag atgcgccaga cgctgacgct gcacaaaggg gcgaattata tttcgctgac
     3241 gcagaacatt cagcaatctg gcttgttaag tgcagaactg caacaaaatg ggcaagtgca
     3301 ggacagcatt agcacaaaac tgtcttttgt ggacaacagc tggcccgttg aacagcagaa
     3361 aaatgtcatg ctcggcggtg gcgagaacgc gctgatgttg cccgagcagg cgagtaatat
     3421 ccggctacaa agtagtgaaa cgccgcagga gattttccgc aacaatcttg atgcgttagt
     3481 cgatggacca tggggtggcg tgatcaacac cggtagccgt ctgatcccgc tcagtctcgc
     3541 ctggcgttcg cttgccgatc atcaaagtgc cgccgctaac gacattcgtc agatgattca
     3601 ggataaccgt ctgcggctga tgcaactggc ggggcccgga gcgcgcttta cctggtgggg
     3661 tgaagatggc aatggtgacg ccttccttac ggcatgggca tggtacgccg actggcaggc
     3721 cagccaggcg ctcggcgtaa cgcaacaacc ggaatactgg cagcatatgc tcgacagtta
     3781 tgccgagcag gcggataaca tgccgttatt gcatcgggcg ctggtgctgg cgtgggcaca
     3841 ggagatgaat ctgccgtgca aaacgttgtt gaaagggttg gatgaagcta tcgcccggcg
     3901 cggaactaaa gatgaagatt tctctgagga agacatccgc gatatcaatg acagcctgat
     3961 cctcgataca ccggaatctc cactggcaga tgcggtggca aacgtcttaa ccatgacgtt
     4021 gctgaaaaaa gcgcagttga agtccacggt gatgccacag gttcagcaat atgcgtggga
     4081 taaagcggca aacagcaatc agccgctggc gcacacggtt gtgctgctca atagcggtgg
     4141 tgacgctacc cagacggccg ctattttaag tggtttgacc gctgagcaat ccactattga
     4201 gcgcgcgctg gccatgaact ggctggcgaa atatatggcg acaatgcctc cagttgtttt
     4261 gcctgcgcct gcgggcgcat gggctaaaca taagttaact ggagggggcg aagactggcg
     4321 ttgggttggt cagggcgtgc cggacattct ctcttttggt gacgaattat cgccgcaaaa
     4381 tgtgcaggtc cgctggcgtg agccggcaaa aatggctcaa caaagtaaca ttccggtgac
     4441 cgttgaacgc cagttgtatc ggcttatccc tggtgaagaa gagatgagct ttattctgca
     4501 accggtgacc agcaatgaga ttgacagcga tgcgctgtat ctcgatgaaa ttacgcttac
     4561 cagcgagcag gatgcagttc tgcgctatgg tcaggtggaa gtaccgctcc cgccgggagc
     4621 cgacgttgag cgcacaacat ggggcatttc agtcaataaa cccaacgccg cgaaacagca
     4681 ggggcaattg ctggaaaaag cgcgtaatga aatgggcgaa ctggcctata tggtgccggt
     4741 gaaagaactg acgggaacgg tcactttccg ccatttgctg cgcttctcgc aaaaagggca
     4801 attcgttctg cctcctgctc gttatgtgcg ttcctatgca cctgcgcagc aaagtgttgc
     4861 ggcagggagt gaatggaccg ggatgcaggt gaaataagtg aactggcgca gaatcgtctg
     4921 gctattggcg ttggttactc tgccaacgct tgcggaggag acgccgttac aactggtgct
     4981 gcgcggtgcg cagcacgatc aactgtatca gctgtcgtca tcaggcgtga ccaaagtctc
     5041 agcattacct gactcgctca ccacgccgtt agggagtttg tggaaactgt atgtttacgc
     5101 ctggctggag gatacccatc aaccggagca gccctaccag tgtcgcggaa actccccgga
     5161 agaggtttat tgttgtcagg ctggggagag cataacgcgc gataccgcgc tggtacgttc
     5221 ctgtgggctc tattttgcgc cgcagcggtt gcatattggc gcagatgtgt gggggcaata
     5281 ctggcaacag cgtcaggcac ctgcgtggtt agcgtctctt acaacgctga agccagaaac
     5341 ctcggtaacg gttaaatcgc tgctcgattc attagcgacg ttacctgcgc aaaacaaagc
     5401 tcaggaagtc ttactggatg tggtgctgga cgaggcgaaa atcggtgtcg catccatgct
     5461 cggtagtcgg gtacgggtga aaacctggag ctggtttgcc gatgataaac aggaaatccg
     5521 ccagggtggt tttgctggct ggttaaccga tggtacaccg ctgtgggtta ctggcagtgg
     5581 caccagcaaa acggtactaa cccgttatgc gacggtgctt aatcgggttt tgccagtgcc
     5641 gacgcaggtt gccagtgggc aatgtgtaga ggtggagctg tttgcccgct atccgctgaa
     5701 gaaaatcaca gcggaaaaga gtacgacggc agtaaaaccc ggcgtactta atgggcgcta
     5761 tcgtgtgacg tttactaatg gtaaccacat tacgtttgtc agccacggtg agacgacctt
     5821 actgagcgaa aagggcaaac tgaaattgca gtcgcatctc gatcgtgaag agtacgttgc
     5881 cagagttctg gatcgcgagg cgaaaagtac gccccctgag gcagcgaaag ccatgaccgt
     5941 ggcgattcgc acttttttac agcaaaacgc caatcgggaa ggcgattgcc tgaccatccc
     6001 cgacagcagc gccacgcagc gtgtttctgc ttcgccagcg accacggggg cgcgaacgat
     6061 gaccgcctgg acgcaggatc tcatctatgc aggcgatccg gttcattatc atggcagtcg
     6121 agctaccgaa gggacacttt cctggcggca agccacggcc caggcggggc agggagagcg
     6181 ttacgaccag atactcgcgt ttgcttatcc cgacaacagc cttagccgct ggggagcgcc
     6241 acgctcaacc tgccagttat tacccaaagc gaaagcctgg ctggcgaaaa aaatgccgca
     6301 gtggcggcgt atattacaag gcgagacggg gtacaacgaa ccagacgtgt ttgcggtctg
     6361 tcgtttagtc tcaggtttcc cctataccga tcggcagcag aaacggctgt ttattcgtaa
     6421 cttctttacg cttcaggatc ggctggattt aacccatgag tatctgcacc tggcctttga
     6481 tggttatccc accgggcttg atgagaacta tatcgaaacg ctgacccgac aattattaat
     6541 ggactaatgc tatgcgaaag atttttcttc cgttgttact ggtggcactt tcgcctgtcg
     6601 ctcacagtga aggtgtgcag gaagtcgaga ttgacgcacc gctctctggc tggcatccgg
     6661 cagagggcga agatgccagt ttttcacaat cgatcaatta cccggcgtcg tccgtcaaca
     6721 tggcagatga tcaaaatatc tcagcgcaga tccgcggcaa aataaagaat tatgctgcgg
     6781 cgggtaaagt tcagcagggt cggctggtgg tcaacggtgc cagtatgccg cagagaattg
     6841 aatccgatgg ttcatttgca cgcccttata ttttcactga aggcagcaac agcgtgcagg
     6901 tcatcagccc ggatgggcaa agccggcaaa aaatgcagtt ttactcaacg ccgggtacag
     6961 gaacgattcg tgcacgttta cggctggttc tctcgtggga tacggacaat accgatctcg
     7021 atctccatgt tgtcacgccc gatggcgaac acgcctggta cggtaacacc gtgctgaaaa
     7081 acagtggtgc actggatatg gatgtcacga cggggtacgg acccgagatt ttcgccatgc
     7141 cagcaccagt tcacgggcgc tatcaggtgt atatcaacta ctatggtggg cgcagcgaaa
     7201 cggaattgac gaccgcccaa ctgacgctga tcaccgatga aggatcggtc aatgagaaac
     7261 aggaaacatt tattgtaccg atgcgtaatg ctggcgagct gacgctggtg aaaagttttg
     7321 actggtgatc catcccttca cacaacctgg ctctctcttt tttcagataa gactgctggg
     7381 cgatctgccg acgccaggat ataaccatta acaagcgtcg gcagattggt catttcttat
     7441 ttttcgtact gagaactgtc catctactcc ggctttcctg gttaaggcaa tggtttctaa
     7501 gcgatacgca ttattgcaat ggtgacagaa cattgcggta acacgctttt accgctacct
     7561 taaccacact ccatcggtca cctgaggcgg agcttcgccc ctttgaaata ccttgcttct
     7621 tttcgtacaa ccctgaaagc ctcgcgctac atgttcagag cattggcgtt agtgctctgg
     7681 ctgttgattg ctttttcatc cgttttttac atcgttaatg cgttacatca gcgagaatcg
     7741 gaaattcgtc aggaatttaa tctgagttcc gatcaggctc agcgctttat tcaacgcacc
     7801 tctgatgtga tgaaagagct gaagtacatc gccgaaaatc gcttatcggc agaaaacggt
     7861 gtgctttccc cgcgtggacg agaaacgcag gcggatgtgc ctgcgtttga accgctgttt
     7921 gccgactccg attgttccgc aatgagtaac acctggcgag gttctctgga gtcattggcg
     7981 tggtttatgc gctactggcg cgataatttt tctgcggctt acgatctcaa ccgggtattt
     8041 ttaatcggca gtgataacct ctgcatggcc aatttcggtc tgcgtgatat gccagtggaa
     8101 cgcgataccg cgttgaaagc gttgcatgaa cgcatcaata aatatcgaaa tgcaccacaa
     8161 gatgatagcg gcagtaacct ctactggatc agcgaaggtc cgcgccctgg cgtcgggtat
     8221 ttttacgcgt tgacgccagt ttatctggcg aaccggttgc aggcgctttt gggtgtcgag
     8281 cagaccatcc ggatggaaaa ctttttctta ccgggtacgt tgccgatggg ggttaccatt
     8341 cttgatgaaa atggtcatac cctgatttcg cttaccggac cagaaagtaa aattaagggc
     8401 gatcctcgct ggatgcagga acgctcctgg tttggctata cggaagggtt ccgggagctg
     8461 gtgctgaaga aaaatctgcc accctcatcg ctaagcatcg tgtattcggt gccggttgat
     8521 aaggtgctgg aacgcattcg catgttgatc cttaacgcaa ttttgctgaa tgtgcttgcc
     8581 ggagctgcat tgtttactct cgcacggatg tacgagcgac gtattttcat tccggcggaa
     8641 agcgacgccc tgcgactgga agaacatgag cagttcaatc gcaagattgt cgcctccgcg
     8701 ccagtgggta tctgcatttt gcgtaccgct gatggcgtca atattttaag taacgaactg
     8761 gcgcatacct atctcaatat gcttacgcat gaggaccgcc aacgactgac gcaaattatc
     8821 tgtgggcagc aggtcaattt tgttgatgtc ctgaccagca acaataccaa tctgcaaatc
     8881 agcttcgtcc attcgcgcta tcgtaatgaa aacgtggcca tttgtgtgct ggtggatgtt
     8941 tcttcgcgcg tgaagatgga agagtcgttg caggagatgg cacaagcagc ggaacaggcg
     9001 agccagtcaa aatcgatgtt ccttgccacc gtcagtcatg agctgcgaac gccgctgtat
     9061 ggcattatcg gtaacctgga tctgttgcaa accaaagagt taccgaaagg cgtcgatcgg
     9121 ctggtgacgg caatgaacaa ctcttccagc ctgttgttga aaattatcag cgatattctc
     9181 gatttctcga agattgaatc ggaacagttg aagatcgaac cgcgtgagtt ttcaccgcgt
     9241 gaagtgatga accacatcac cgccaactat ttaccgctgg tggtacgcaa gcagttaggc
     9301 ttgtactgct ttattgaacc ggatgtgcca gtggccttaa atggcgaccc gatgcgttta
     9361 cagcaggtca tctccaacct gctgagtaac gccataaaat tcaccgatac cggctgtata
     9421 gttttgcatg ttcgcgctga tggcgattat ctctctatcc gtgttcgcga taccggcgtg
     9481 ggcattccgg cgaaagaagt ggtgcgcttg tttgatccct tcttccaggt cggaacgggc
     9541 gtacagcgta acttccaggg gaccggtctg ggtctggcga tttgtgaaaa actgatcagc
     9601 atgatggacg gcgatatctc ggtagattca gaaccgggaa tgggcagcca gtttaccgtg
     9661 cgtattccgt tgtacggcgc tcagtacccg cagaaaaaag gcgtggaagg gttgagtggt
     9721 aaacgctgct ggctggcggt ccgcaatgcg tcgctctgtc agttcctgga aaccagtttg
     9781 cagcgcagcg gcatcgtcgt tacaacatac gaagggcagg aaccgactcc cgaagatgtg
     9841 ttaatcactg acgaggtagt gagtaaaaaa tggcagggta gagcggtagt gaccttctgt
     9901 cgtcgccata ttggtattcc gctggagaaa gcgccagggg agtgggtaca cagtgtggct
     9961 gctccgcatg agctaccggc attgttggcg cgtatttatt tgatcgagat ggagagcgac
    10021 gatcctgcta acgctctgcc gtcgacggac aaagcggtca gcgataacga cgatatgatg
    10081 attctggtcg tggatgatca tccgattaac cgacgtctgc tggcagatca gttgggatcg
    10141 ttgggctatc aatgtaaaac cgcgaatgat ggcgtcgatg cgcttaatgt acttagcaag
    10201 aatcatattg atatcgtgct tagcgacgtc aacatgccaa atatggatgg ttaccgcttg
    10261 acgcaacgca ttcgtcagtt gggactgacg ttgccggtaa tcggagtaac tgctaatgcg
    10321 ttggctgaag agaagcagcg gtgtctggag tccggtatgg acagctgcct gtcgaagccg
    10381 gtaacgctgg atgtgataaa acagacgctg acggtatatg ccgagagggt caggaaatcg
    10441 cgggaatcgt aggacggata aggcgtttac gccgcatccg gcattcagtg caaatgccag
    10501 ataagacact aacgcgtctt atctggccta caggtgatta gtctttatct gccggactta
    10561 acgtcacaga agagagataa ttcagcaggg cgatatcgtt ctcgacaccc agcttcatca
    10621 tcgcagattt cttctggcta ctgatggttt taatactgcg gttcagcttt ttagcgatct
    10681 cggtcaccag gaagccttcc gcaaacaggc gcagaacttc actctctttt ggcgagagac
    10741 gcttgtcacc gtaaccacca gcactgattt tttccaacag gcgagaaacg ctttccgggg
    10801 taaatttctt ccctttctgc agcgcggcga gagctttcgg cagatcggtc ggtgcacctt
    10861 gtttcagcac gatcccttcg atatccagat ccaataccgc actaagaatc gccgggttgt
    10921 tgttcatggt cagaacaata atcgacaggc ttgggaaatg gcgcttgatg tacttgatta
    10981 aggtaatgcc atcgccgtac ttatcgccag gcatggagag atcggtaatc aacacatgcg
    11041 catccagttt cggcaggttg ttgatcagtg ctgtagagtc ttcaaattcg ccgacaacat
    11101 tcacccactc aatttgctca agtgatttgc gaataccgaa caagactatc ggatggtcat
    11161 cggcaataat tacgttcata ttgttcatgt aataggctac cttgctacag caagctcttg
    11221 acataactgt caatgtcgct gatgtatttt tctattcctg gaacatcctt ctcacgaatc
    11281 agatgttcca gcgtttcaca taactgcttg ccgggtacca gatttagcat ggcaaatacg
    11341 cctttaagac gatgagccgt ttgggctaac gcagcaaagt cactggttgc tgcttcagta
    11401 tacagcctct taacatcatc cggtactgtg tctacaaaga gcgcataata gccgctggca
    11461 tggagttgcg cattttcatc tccgcccaga ggcgattctg tcacctcttc ctgcgccagt
    11521 tgcacttcaa ttaattgtaa gaccgcttcc tgcatagcgt tgctcatatt gaagttgacg
    11581 cacaattgac caggcccaat ttcccgtacg ccagactcat catcgcttaa aagcaagcca
    11641 gaggcagtaa gattagacgg attatccgtt aaaaagatat cataatcttg actaattaat
    11701 ctttcatcgg gtgtgataca ggttgcaccc caattttcta actggcgagt gacaatattc
    11761 cgaatttctg ccgaagtaac atccaccatt acgcagacat catccagtaa acgctcttct
    11821 tcctcttcaa cttccgggtc agcggcgagc attttgatat gcacagagta gcgtgtacca
    11881 agcccatccc gcgttttgat gtttaaatga ccgcccagtt tacgcgccag ttgatcgctc
    11941 agccagaatg ccagcgggtc cgccttgcca tagcgatcgt tttgggtctg gttgataaac
    12001 gggaagtgca aattatccat ttcatgaata cttacgcctt ctccggtgtc cagaatgcgg
    12061 aatgtcaggc ggtcttcaga ggactcatcc tgatcaacct caagggtgat ttttcccaat
    12121 tgcgttgagg tcacggcata ttgcatcagt agcagcaaaa tacgtcgtaa ggcatcgcga
    12181 tcgccgcggc gcatatcgtg tgctttcaga tgattgttaa tcagcagttg cagaccttta
    12241 cgcttgatgg caggcaacac tgaaggcaca acttcatcaa ttaaatcctg cacggagaac
    12301 agcaccgtct cacttttcca gctatcatcc gcaagcatgt tcgctaactg tatttcatca
    12361 accagccgca ccagcacatc tgcctgattc gccagttgtt tgctttccgg ggcgttgagt
    12421 ttagccgcgc tctccgccag ggactgtgca ggttctttca gcgcatcgcc aatgtttttc
    12481 ataaaggtca tccggccctg ctggtttttc tcatacagac gctgcgcctg cttgagtttc
    12541 ttgtttacca gcacttcgcg atcctgatcg cgaataatga aaatttgtgt gcgcggcgcg
    12601 acctggctgc ggaacatgcg gatctcatac agctcgttat tgatcgtcgc ctgaataatc
    12661 ccctgatgct gttccgccat ggtggtgatg ttttgcagat tcaaatgcgg cagcaaatga
    12721 tcggcaattt tgttacttat gacagtgcgg ttcgattcct gatcgtgaac cagcaggccg
    12781 agcggcagca gtgagactat ctcttcatta attgcccgta aaatgcgcaa ttcgttattg
    12841 accgccgtgc tgggtagact ttctgtactg cggctggaga aatggcggaa tgtggtatag
    12901 ccaaataacg ccagcgccag caaaccgatg ttcagcagca gtggcagcag aatgttttgc
    12961 aacgtatcca gcaataaggt gccataagga acctgccaga ccaagcgcat atcggtagag
    13021 ttgagtgccg aggagatttc aatcttcgta ctgttaaagt ggatactgac gctatccgtc
    13081 ccttctttct cattatcatt gtttcccgtt gccgtcgcgt ctggctcaag gcggaaactg
    13141 tccagcggca tacccggtgg gatcaaatca ttaatcggca gatcaaaagc cacgaccgtt
    13201 gccagatgtc ctggctggtt aaaggtagta cgcaaggtaa agtaatgacc gttctgccag
    13261 gccaggcggc gcatgttaga aaagctttcg cgttcatcga gggcgttggc ctgttgcaac
    13321 atctctgcac gacgtgaatc aacaatgtcg ctgacggtcg attctttaaa tccggaggtg
    13381 agatctttaa ggggtagggt tgagatcagc accagactgt tatcctgacc attcaggtaa
    13441 tacatcgacc acggtacatt ttctgcgccc cacaatgtat ccagataagt ggacatccgc
    13501 tgagtcatct caagcgttga gttgtcgtga gagccaaaga ttaacgcttc cgttttgcgg
    13561 cgtggtttct ccagatagta gacatcctgt ttcaggcgcg tctcttgtaa accttcgccg
    13621 gaggaggggg aggtcgtcgc ggcaatgttg tcgtagatct gccaggtcac gtaacgccag
    13681 gtatcgacgc gcttttggat agcgtgggta atgtcgacaa tctggtaact tttatctttc
    13741 agccatgcgt taacggcgct ttgtaccatt acacccatcg tcaccagtaa cacaatgatc
    13801 aacagtaaaa agaagcgggt aatgctcccc ggtaggagtg aaaagcgggt cgtggccgtt
    13861 gtctctttct gacgcattcg tgtttatgac ctgttaaaac ttcgcgaagt gatagggcag
    13921 tataaagggt acagtgtgaa attccagact cttacctgat tgatgattct ctattctccc
    13981 gtgatattta tcatcctgca ttgggggaaa tagatgtaca agcgccattt tgctgtacaa
    14041 ataacctaca aaaagcccaa cgaaaataat tgcaaataaa caaagattgc tggaaattat
    14101 gctgatgtta attatttgtg aaatagttaa caagcgttat agtttttctg tggtagcaaa
    14161 gaataatgaa aagtgtgtaa agaagggtaa aaaaaaccga atgcgaggca tccggttgaa
    14221 ataggggtaa acagacattc agaaatgaat gacggtaata aataaagtta atgatgatag
    14281 cgggagttat tctagttgcg agtgaaggtt ttgttttgac attcagtgct gtcaaatact
    14341 taagaataag ttattgattt taaccttgaa ttattattgc ttgatgttag gtgcttattt
    14401 cgccattccg caataatctt aaaaagttcc cttgcattta cattttgaaa catctatagc
    14461 gataaatgat acatcttaaa agttttagta tcatattcgt gttggattat tctgcatttt
    14521 tggggagaat ggacttgccg actgattaat gagggttaat cagtatgcag tggcataaaa
    14581 aagcaaataa aggcatataa cagagggtta ataacatgaa agttaaagta ctgtccctcc
    14641 tggtcccagc tctgctggta gcaggcgcag caaacgctgc tgaagtttac aacaaagacg
    14701 gcaacaaatt agatctgtac ggtaaagtag acggcctgca ctatttctct gacaacaaag
    14761 atgtagatgg cgaccagacc tacatgcgtc ttggcttcaa aggtgaaact caggttactg
    14821 accagctgac cggttacggc cagtgggaat atcagatcca gggcaacagc gctgaaaacg
    14881 aaaacaactc ctggacccgt gtggcattcg caggtctgaa attccaggat gtaggttctt
    14941 tcgactacgg tcgtaactac ggcgttgttt acgacgtaac ttcctggacc gacgtactgc
    15001 cagaattcgg tggcgacact tacggttctg acaacttcat gcagcagcgt ggtaacggtt
    15061 tcgcgaccta ccgtaacact gacttcttcg gtctggttga cggcctgaac tttgctgttc
    15121 agtaccaggg caaaaacggt agcgtaagcg gcgaaggcat gactaacaac ggtcgtgaag
    15181 cactgcgtca gaacggcgac ggcgttggcg gatctatcac ttatgattac gaaggcttcg
    15241 gtatcggtgc tgcagtttcc agctccaaac gtactgatga tcagaacagc ccgctgtaca
    15301 tcggtaacgg cgaccgtgct gaaacctaca ctggtggtct gaaatacgac gctaacaaca
    15361 tctacctggc tgctcagtac acccagacct acaacgcaac tcgcgtaggt tccctgggtt
    15421 gggcgaacaa agcacagaac ttcgaagctg ttgctcagta ccagttcgac tttggtctgc
    15481 gtccgtccct ggcttacctg cagtctaaag gtaaaaacct gggtcgtggc tacgacgacg
    15541 aagatatcct gaaatatgtt gatgttggtg cgacctacta cttcaacaaa aacatgtcca
    15601 cctatgttga ctacaaaatc aacctgctgg acgacaacca gttcactcgt gacgctggca
    15661 tcaacactga taacatcgta gctctgggtc tggtttacca gttctaatct cgattgatat
    15721 cgaacaaagg gcctgcgggc ccttttttca ttgttttcag cgtacaaaca cagttttttg
    15781 gtgtactctt gcgaccgttc gcatgaggat aatcacgtat ggaaataagc tttacccgcg
    15841 tggcactgct ggctgccgcg ctcttctttg ttggttgcga tcaaaaacca caaaccgcca
    15901 aaacccacgc tactgaagtt accgttcttg aaggcaaaac tatgggtacc ttctggcgtg
    15961 ccagcatccc gggcattgac gccaaacgca gtgccgaact taaagaaaag attcagaccc
    16021 agctggacgc cgacgatcag ctgctttcga cctataaaaa agattccgcg ctgatgcgct
    16081 ttaacgattc gcaaagttta tcgccgtggc cggtaagtga agcgatggcc gatatcgtca
    16141 ccacctcgct gcgcattggc gcaaggaccg atggtgcgat ggatataacc gtcgggccgc
    16201 tggtgaatct gtggggcttt ggcccggaac aacagccggt tcaaattccg agccaggaac
    16261 agatcgatgc gatgaaagcc aaaaccggct tacagcacct gacggtcatt aatcagtcgc
    16321 atcagcaata tctgcaaaaa gacctaccgg atttatatgt cgatctctcc accgtcggtg
    16381 aaggttatgc ggcggatcac ctggcacgct tgatggagca ggaagggatt tcccgctatc
    16441 tggtgtcggt gggcggcgcg ctgaacagcc gtggtatgaa cggtgaaggc ctgccgtggc
    16501 gggtagcgat tcaaaaacca accgataaag aaaacgcggt tcaggctgtg gtggatatca
    16561 acggtcatgg tattagcacc tctggcagtt accgtaacta ttacgaactg gacggcaaac
    16621 gtctttctca tgttatcgat ccgcaaaccg ggcgtcccat cgaacacaat ctggtatccg
    16681 tgacggtgat tgctccgacg gcgctggaag ccgatgcctg ggatactggc ttgatggtac
    16741 tcgggccgga gaaagccaaa gaggttgttc gccgggaagg gctggcggtt tatatgatca
    16801 ccaaagaagg cgatagcttt aaaacctgga tgtcaccaca gtttaaaagc ttccttgtca
    16861 gcgaaaaaaa ttaaagcgca agattgctgg tttttgcgtg atggtgaccg ggcagactaa
    16921 aggctatcct taaccaggga gctgattatg aaaaacgcca catgcttaac tgacgatcaa
    16981 cgctggcaat ctgtcttagc ccgcgacccg aatgccgaca gcgaattcgt tttcgccgtg
    17041 cgcaccacag gcatcttttg ccgtccgtct tgccgcgcca gacatgcctt gcgggaaaat
    17101 gtctccttct acgcaaatgc ctgcgaggca ctcgccgccg gatttcgccc ctgcaaacgc
    17161 tgccaacctg ataaagccaa tgcccagcaa catcggttgg ataaaatcac ccacgcgtgt
    17221 cgactgctgg aacaggaaac gcctgtaacg ctggaagcct tagccgacca ggtggcgatg
    17281 agtccatttc atctacatcg gttgtttaaa gcgactaccg gaatgacgcc taaagcctgg
    17341 caacaggcct ggcgcgctcg ccgtttgcgc gaatcgctgg cgaaagggga gagcgtgacg
    17401 acgtctattc ttaacgccgg attccccgac agcagcagtt actatcgcaa agctgacgaa
    17461 acgctgggca tgacggctaa acaattccgt cacggtggcg aaaatctggc ggtgcgttac
    17521 gcgctggctg attgtgagct gggtcgttgc ctggtggcag aaagcgagcg ggggatttgc
    17581 gcgatattgc tgggcgatga tgacgcgaca ctaatcagcg agttgcagca gatgtttccc
    17641 gctgccgaca acgcgcctgc cgatctgatg tttcagcaac atgtgcgtga agtgatcgcc
    17701 agcctcaatc aacgcgatac gccgctgacg ttaccgctgg acattcgcgg cactgctttt
    17761 cagcaacaag tctggcaggc actgcgcacg ataccttgcg gtgaaaccgt cagttatcag
    17821 caactggcta acgccatcgg caaaccgaaa gcggtacggg ccgttgccag cgcctgtgcc
    17881 gccaacaagc tggctatcat aataccctgt catcgggtgg tccgtggtga tggcacactt
    17941 tccggttacc gctggggcgt gtcgcgtaaa gcgcaactgc tgcgccgcga agctgaaaat
    18001 gaggagaggt aatgttggat ctgtttgccg atgctgaacc gtggcaagag ccactggcgg
    18061 ctggagcggt aattttgcac cgttttgctt ttaacgctgc ggagcaactg atccgcgata
    18121 ttaatgacgt tgccagccag tcgccgtttc gccagatggt cacccccggg ggatatacca
    18181 tgtcggtggc gatgaccaac tgtgggcatc tgggctggac gacccatcgg caaggttatc
    18241 tttattcgcc cattgatccg caaacaaata aaccgtggcc cgccatgcca caaagctttc
    18301 atgatttatg tcaacgtgcg gctacggcgg cgggctatcc ggatttccag ccagatgctt
    18361 gtcttatcaa ccgctacgct cctggcgcga aactgtcgct gcatcaggat aaagacgaac
    18421 cggatctgcg cgcgccgatt gtttctgttt ctcttggctt acccgcgatt tttcaatttg
    18481 gtggcctgaa acgtaatgat ccgctcaaac gtttgttgtt ggaacatggc gatgtggtgg
    18541 tatggggcgg tgaatcgcgg ctgttttatc acggcattca accgctgaaa gcggggtttc
    18601 atccactcac cggcgactgc cgctacaacc tgacattccg tcaggcaggt aaaaaagaat
    18661 aaaaataaga attattattg ctgtgcgcgc gaagatgttt aaactgcggg ctgtaattca
    18721 ttgtccgggt tttctgcatg gaacttcttg tacttgtctg gcggcagtat cgctggccat
    18781 ttatcagtgt gatggcgcta agcctcgcca gtgcggcatt aggcattggc ttaattgctt
    18841 ttatcaatca gcgccttatc gaaacggcgg ataccagtct gctggtattg ccggagtttc
    18901 tgggattatt gctgctgttg atggcagtca ctctcggatc gcaactggcg ctcaccactt
    18961 tggggcatca cttcgtttac cgactgcgta gtgaatttat caagcggatt ctggatacca
    19021 acgtcgagcg cattgaacaa ctcggtagcg cctcgttgct ggcgggatta accagcgatg
    19081 tgcgcaatat caccattgct tttgtgcgtc ttccggaact ggtgcagggg atcattctca
    19141 ctatcggttc cgcggcgtat ctgtggatgc tgtcgggcaa aatgttgctg gtaacggcta
    19201 tctggatggc gatcaccatc tggggtggtt ttgtactggt ggcgcgggtg tacaaacata
    19261 tggcgaccct gcgtgaaacc gaagacaggc tgtatacgga ttttcaaact gtactcgaag
    19321 ggcgcaaaga gctgactctg aaccgggaac gcgccgagta tgtgtttaac aacctctaca
    19381 ttcctgatgc gcaagagtat cgccaccata ttatccgcgc agacaccttc catcttagcg
    19441 ccgtgaactg gtcaaacatc atgatgttgg gcgcaatcgg cctggtgttc tggatggcga
    19501 acagtctcgg ttgggctgat accaacgttg ccgcgaccta ttcgttgacg cttttattcc
    19561 tgcgtacgcc gctgctttcg gcggttggcg cattgccgac gctgctgacg gcgcaggtgg
    19621 cgtttaacaa gctgaacaaa ttcgcgctcg cgcctttcaa agcagagttt ccgcgcccgc
    19681 aggcgtttcc caactggcaa acgctggagc tgcgtaacgt gacgtttgct tatcaggata
    19741 acgcgttttc cgttggtccg attaatctca ccatcaaacg tggcgagctg ctgtttctga
    19801 ttggcggcaa cggtagcgga aaatcgacgc tggcgatgtt gttgacgggc ttgtatcagc
    19861 cacaaagcgg cgaaatcttg ctggatggca aacctgtcag cggcgaacaa ccggaagatt
    19921 atcgcaaact gttttcggca gtgtttaccg atgtctggct gtttgatcaa ctgctggggc
    19981 cggagggtaa acccgctaac ccgcaactgg ttgagaagtg gctggcgcag ctgaaaatgg
    20041 ctcataagct tgagttaagc aacgggcgta ttgttaacct gaagttatca aaagggcaga
    20101 aaaaacgcgt ggcgctgttg ctggcgctgg cagaagaacg cgatattatc ctgctggatg
    20161 aatgggcggc ggatcaggat ccacacttcc gtcgtgagtt ttatcaggtg ttgctgccgc
    20221 tgatgcagga gatgggtcaa actattttcg ccatcagtca tgatgatcat tactttatcc
    20281 acgccgaccg cctgctggaa atgcgcaacg ggcaacttag cgagctgacg ggcgaagagc
    20341 gcgatgccgc ttcgcgtgat gccgttgccc ggacggcata atattacgac gctgacctgg
    20401 cggactgctg ccgtcaggtc aatatgctaa aaatccctt
//
LOCUS       DAZGJW010000080        20433 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZGJW010000080 DAZGJW010000000
VERSION     DAZGJW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.080"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..908)
                     /locus_tag="ACHKIK_002959"
     CDS             complement(<1..908)
                     /locus_tag="ACHKIK_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO9084095.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKP"
     gene            complement(920..2134)
                     /gene="rspA"
                     /locus_tag="ACHKIK_002960"
     CDS             complement(920..2134)
                     /gene="rspA"
                     /locus_tag="ACHKIK_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO9084096.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(2340..2666)
                     /gene="ynfA"
                     /locus_tag="ACHKIK_002961"
     CDS             complement(2340..2666)
                     /gene="ynfA"
                     /locus_tag="ACHKIK_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO9084097.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            2801..3142
                     /gene="ynfB"
                     /locus_tag="ACHKIK_002962"
     CDS             2801..3142
                     /gene="ynfB"
                     /locus_tag="ACHKIK_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO9084098.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            3177..3737
                     /gene="speG"
                     /locus_tag="ACHKIK_002963"
     CDS             3177..3737
                     /gene="speG"
                     /locus_tag="ACHKIK_002963"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO9084099.1"
                     /translation="MPSAHSVKLRPLEREDLRYEHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(3740..4450)
                     /gene="ynfC"
                     /locus_tag="ACHKIK_002964"
     CDS             complement(3740..4450)
                     /gene="ynfC"
                     /locus_tag="ACHKIK_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO9084100.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            4558..4863
                     /gene="ynfD"
                     /locus_tag="ACHKIK_002965"
     CDS             4558..4863
                     /gene="ynfD"
                     /locus_tag="ACHKIK_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO9084101.1"
                     /translation="MKLSTCCAALLLALASPVVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            5062..7487
                     /gene="ynfE"
                     /locus_tag="ACHKIK_002966"
                     /pseudo
     CDS             5062..7487
                     /gene="ynfE"
                     /locus_tag="ACHKIK_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
     gene            7548..9971
                     /gene="ynfF"
                     /locus_tag="ACHKIK_002967"
                     /pseudo
     CDS             7548..9971
                     /gene="ynfF"
                     /locus_tag="ACHKIK_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
     gene            9982..10599
                     /gene="ynfG"
                     /locus_tag="ACHKIK_002968"
     CDS             9982..10599
                     /gene="ynfG"
                     /locus_tag="ACHKIK_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO9084102.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            10601..11455
                     /gene="ynfH"
                     /locus_tag="ACHKIK_002969"
     CDS             10601..11455
                     /gene="ynfH"
                     /locus_tag="ACHKIK_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO9084103.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            11498..12112
                     /gene="dmsD"
                     /locus_tag="ACHKIK_002970"
     CDS             11498..12112
                     /gene="dmsD"
                     /locus_tag="ACHKIK_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO9084104.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            12271..13563
                     /gene="clcB"
                     /locus_tag="ACHKIK_002971"
     CDS             12271..13563
                     /gene="clcB"
                     /locus_tag="ACHKIK_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO9084105.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(13516..14211)
                     /gene="bioD"
                     /locus_tag="ACHKIK_002972"
     CDS             complement(13516..14211)
                     /gene="bioD"
                     /locus_tag="ACHKIK_002972"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO9084106.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(14336..15556)
                     /gene="mlc"
                     /locus_tag="ACHKIK_002973"
                     /gene_synonym="dgsA"
     CDS             complement(14336..15556)
                     /gene="mlc"
                     /locus_tag="ACHKIK_002973"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO9084107.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(15691..16584)
                     /gene="ynfL"
                     /locus_tag="ACHKIK_002974"
     CDS             complement(15691..16584)
                     /gene="ynfL"
                     /locus_tag="ACHKIK_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9084108.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRYTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            16691..17944
                     /gene="ynfM"
                     /locus_tag="ACHKIK_002975"
     CDS             16691..17944
                     /gene="ynfM"
                     /locus_tag="ACHKIK_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9084109.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(17993..18112)
                     /locus_tag="ACHKIK_002976"
     CDS             complement(17993..18112)
                     /locus_tag="ACHKIK_002976"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO9084110.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            18368..18676
                     /gene="asr"
                     /locus_tag="ACHKIK_002977"
     CDS             18368..18676
                     /gene="asr"
                     /locus_tag="ACHKIK_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO9084111.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            18769..18852
                     /locus_tag="ACHKIK_002978"
     CDS             18769..18852
                     /locus_tag="ACHKIK_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084112.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            18952..19773
                     /gene="ydgD"
                     /locus_tag="ACHKIK_002979"
     CDS             18952..19773
                     /gene="ydgD"
                     /locus_tag="ACHKIK_002979"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO9084113.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(19812..20141)
                     /gene="mdtI"
                     /locus_tag="ACHKIK_002980"
     CDS             complement(19812..20141)
                     /gene="mdtI"
                     /locus_tag="ACHKIK_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO9084114.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(20128..>20433)
                     /gene="mdtJ"
                     /locus_tag="ACHKIK_002981"
     CDS             complement(20128..>20433)
                     /gene="mdtJ"
                     /locus_tag="ACHKIK_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO9084115.1"
                     /translation="SMKWASVSEGNGGFILMLVMISLSYIFLSFAVKKIALGVAYALW
                     EGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLIKSGTRKARKPELEVNHGAV"
BASE COUNT         5133 a         5256 c         4978 g         5066 t
ORIGIN      
        1 ggtttaatta accctttact taaccagtcg ataacaaccg gaaatttatt tgcatttaag
       61 cgtgaagaga aaatagagag ttcttttccg gtaattcctt gctgaatcac ttcagacggt
      121 tcactggaga agcccatcaa tacaatacgt gccgctggag aagccagcgt tacggcctct
      181 ttcaggatag aaggatgaca agccgcatcg ataattaatg tcggcttgat gcctttttca
      241 gcgaaaatct cgccaagcgg tgtctggctg ttattaatcg cccagtctgc cccgctctct
      301 ttcgcttttt ccagtcgttc atcaatgcga tcggcaacaa tcacattttt aacgttatag
      361 acgcctttta atacctgaac gatcgtcagg ccgattggac cggcaccata aaccagaacg
      421 gtatcatttt cagtcggttg accatgaccg gtaacgttag ccgcaatggt aaaaggttcg
      481 atcatcaccg catattgatc ggccactgct tcaggaattt tccacgcatt ttttgccgga
      541 accacggcat attcactgaa accaccgtca gcgtgcacac ctaatacagc aagtgtcgta
      601 caaacgttcg gcttacctat agagcacgga tagcaatgcc cacagctgac caccggatcg
      661 acagcaacgc gctcaccgac tctggcgctt tccacacctt cacccaccgc atcaatgacg
      721 ccaaagaatt catgaccaat gacgcgcgga tatttcgcaa aaggattatg cccacggtaa
      781 atatggctat ctgaaccaca aattccggca agtttcactt ttactcgtac ttcacccgct
      841 gacggggtgg gtatttcacg ttcgataatc gacagttgat tcggtttttc aattaaaatg
      901 cttttcatta ccttactcct taccagttcc acagcgtgcc atcttccaga cgtgcgactg
      961 gcagataagc aggttcatag ggatatttcg ccgccagctt ttcatcgaat tcgatgccaa
     1021 gacccggttt gtctcccgga tgcatatagc cgttatcgaa agtccagttg tgcgggaaga
     1081 cttcgagcat ttgttcggaa taacccatgt actcctggac accgaaattg gggacccaca
     1141 gatcaaagtg cagcgccgca gccatgcaga ctggtgacaa atcggaagga ccgtgtgagc
     1201 cagtacgtac ctgatacagc gaagcaaaat cggcaatccg gcgcataccg gtaattccgc
     1261 ctgcatgggt cagcgtggtg cggatataat cgatgagttg ctcttcaatc agttgtttgc
     1321 agtcccagat gctgttgaag acttcaccca ctgcgatggg tgtgacggta tgttggcgaa
     1381 tgagacggaa gcattcctgg ttttccgcag gcgtcgggtc ttccatccag aacatgcgat
     1441 aatcttcaat gcttttacca aagcgcgccg cttcaatagg cgttaagcga tggtgcatgt
     1501 catgcagcaa atgttcatta aaaccaaact tgttacgtac cgcgtcaaac aatttcggca
     1561 tgaaatcgag gtatttctcc gtcgaccaca gctgctcttc cggccactgt cctttggttg
     1621 cgggttcata agccagacct ttacctttcg acatgccgta ggtggttttc ataccaggga
     1681 ttccgcactg cacgcggatg gctttgaatc ccagctcctg atgacgggca taatcatcca
     1741 gagcttcatc aatactgtga ccggtggtat ggcaataaac catcacccct tcacgagacg
     1801 cgccgccgag taactggtaa agcggcatgt tggcagcttt ggctttaata tcccacagcg
     1861 ccatatcaac cgctgaaatg gccgacatcg taaccggacc gcgacgccag taagcacctt
     1921 tatagaaaaa ctgccagata tcttcgatac ggtgcgcatc gcgaccaata agctgcggac
     1981 aaaggtgatc ctgcaaataa gaggccacgg aaagctcacg tccattgagg gtggcatccc
     2041 caaggcccgt aataccgtcc tcagtggtga tttttaatgt gacgaaatta cgccccggac
     2101 aggtaacaaa aacttcagcc tttacgatct tcatgttcga ttccttgcat cgcttgtcgt
     2161 gatgcatgaa atctacgcaa ctgagctact accatacaag cataaagatc gaaaaaagcc
     2221 ggagtgatca caaaaaaagg cgtatatttg cgctgtgaat ggttgacaaa agatgaaata
     2281 gaataccttt tgtcagctga cacttcctct tatcttattg ataaaatgga tttatgttcc
     2341 tacgtgcgcc cccagcccgc aacaatgatc aacatgccgc aaagcgcaat caacgcaccc
     2401 gtccagtcat aaagagtcag tttcacgcca tccacaacgc gcagccacat caacgccgtg
     2461 cagacataaa cgccaccata agccgcgtaa acacgcccac tcgccgctgg atgcaacgtt
     2521 aacaaccaga caaacagcgc cagtgaaatc cccgccggaa gcaacagcca gatactggcg
     2581 tttcgtttta accacaacca gggcagaaag catccaataa tttcacacag cgcagtagca
     2641 aaaaatagta acgttgtttt aatcatcttt gtctcttatt gacatcatgt atagttatag
     2701 ggcgacataa tatcatcaat ataaacaccc tcctggtacg ttttgcgtcc gcagtggatg
     2761 gtgtagaatc acctttaatc attcatacag ggaatgaatt atgaaaatca ctctcagcaa
     2821 acgaatcggc ctgctcgcta ttctgctgcc ttgcgcactg gcattgagca caactgttca
     2881 tgccgaaact aacaaactgg tgattgagtc tggcgacagt gcacaaagcc gccagcacgc
     2941 cgctatggaa aaagagcaat ggaatgacac gcgcaatctg cgccagaaag tgaataaacg
     3001 cactgaaaaa gagtgggata aagccgacgc cgcttttgat aaccgcgata aatgtgagca
     3061 aagcgccaac atcaatgcct actgggagcc caatactttg cgctgcctgg accgtcgaac
     3121 tggccgcgtt attaccccct aacctgttat tgatttaagg aatgtaagga cacgttatgc
     3181 caagcgccca cagtgttaag ctacgcccgc tggagcgtga agatttacgc tatgaacatc
     3241 aactcgacaa caacgccagt gtgatgcgtt actggtttga ggaaccctac gaagcctttg
     3301 ttgaactctc tgatctgtat gataagcata ttcacgatca gagcgaacgg cgctttgtgg
     3361 tggaatgtga cggcgaaaaa gccggtctgg tggagctggt ggaaattaac catgttcatc
     3421 gccgcgcaga atttcagata attatctccc cggagtatca ggggaaaggt ctggcaaccc
     3481 gtgccgccaa attagcaatg gactatggct ttaccgttct caatctctat aagctgtatc
     3541 tgatcgttga taaagagaat gaaaaagcga ttcacattta ccgcaagctt ggcttttcgg
     3601 ttgaaggtga attgatgcac gagttcttta ttaatggtca atatcgtaat gccattcgca
     3661 tgtgtatatt ccagcatcag tatctggcag agcacaaaac accgggtcag actctcctga
     3721 agccgaccgc acaatagcat taataataat cgatcgtatt tttgatggtg taaacccgtt
     3781 cgacggcggg ttttactcct tcatcaacaa tgattagctg acagtccacc ggattagcgt
     3841 gactgtcata ttcacagctc tgttttacat taccaacccg ttgattattc agtaaagtaa
     3901 ccgctgtgta atctaatttt ttgatcggat ccgttgatgg cgtggcgctg acagataatg
     3961 ttttgtcgtt acttttcgtg gttttaccca gcggataacc ctgatcatca tagcgatatt
     4021 ccatctgcat ttgtttgctg ctggctttaa tcacgaagcc attatcatcg gtttcccaac
     4081 tcaccccggc agaaggtaat tctgctagct ggcattttcc ctgtaaacgt actctcttct
     4141 ccagcgtctc ggcatcacgg taataattgg cgtccagtac cagagcgacc acggtattat
     4201 tttccagatc cagtaattcg agtgaatcaa aacagccttc ttccgacaaa gtcccagaaa
     4261 cacgtttcgt cacttcacct tgctcatcca ttaatgtctg agtgaaatct tttaccggac
     4321 cacgcagcgg atcaaaatcg aattcattag agaaactggc catctcaggg gtaaatgaaa
     4381 gtgttacctc tgtgcggtca catcctgtga ggaatatcgc gagtaagcat ggtagtaatt
     4441 tgtatttcac aacagtcacc agagagtaga gatgattctc aatcatagta gcaaatacag
     4501 tactttacac gttaaatgct atgcttaaag aagttatctt cgcgtaagga gcttatgatg
     4561 aaactctcaa cctgctgtgc cgcgcttctg ctcgccctcg cctcacccgt ggtactcgcc
     4621 gcgccgggat cctgtgagcg catacagagc gatatatcac agcgcattat caataatggc
     4681 gtacccgaaa gcagcttcac gttaagtatt gtacccaatg accaggttga tcagcctgat
     4741 tcccaggtcg tcggccattg cgctaatgat acgcataaaa ttctctatac ccgcaccacc
     4801 agcggtaacg tctctgctcc cgcgcagtct acccaggatg gcgcgcctgc cgaaccgcag
     4861 taatacatta tcgactgaac gccggatacg acacatccgg cgatttgaac atacaacata
     4921 atcccacctt attactcata cccttctatt gatatggatt aataattctt aacccaaaat
     4981 gggtagactc cctctattgt tagcgcgcta aatattcaat atataaacat ttatataacg
     5041 ataaagaaca gggagtgagt tatgtccaaa aatgaccgaa tggtgggcat cagccgcaga
     5101 acactcgtta aatcaaccgc gataggttct ctggcgctgg ctgcaggcgg tttttcttta
     5161 ccgttcaccc tgcgcagtgc agcggcaacg gtacaacagg ccagcgaaaa agtgatctgg
     5221 ggtgcctgtt ccgtcaactg tggtagccgc tgtgcacttc gtctacatgt taaagataat
     5281 gaagtgacct gggtggaaac tgacaatacc ggcagcgatg agtacggcaa ccatcaggta
     5341 cgcgcctgtt tgcgcggtcg ctccatccgc cggcgtatta atcatcccga tcgcttgaat
     5401 tacccaatga aacgcgtggg tacacgcggt gaaggcaaat tcgaacggat tagctgggat
     5461 gaagccctgg atactatcgc cagtagcctg aaaaaaaccg tcgaacaata tggcaatgag
     5521 gctgtatata ttcagtactc ttcggggatt gttggcggca atatgacccg ctcttcgcca
     5581 tcagcctcgg cggtcaaacg cctgatgaac tgctacggcg gttcactcaa tcagtatggc
     5641 tcctacagca ccgcgcaaat ttcctgtgcc atgccctaca cctacggcag taatgatggc
     5701 aacagcacca cggatattga aaacagcaag ctggtcgtga tgtttggtaa caacccggca
     5761 gaaacccgca tgagcggcgg tggcatcact tatcttcttg aaaaagcgcg cgagaaatcg
     5821 aacgccaaaa tgattgttat cgatccgcga tataccgata cggctgctgg tcgtgaagat
     5881 gaatggctcc ctattcgccc gggcaccgat gccgcgctgg ttgcgggtat tgcctgggta
     5941 ttgattaacg aaaatctcgt tgatcaacct tttctcgata aatactgcgt cggctatgac
     6001 gaaaaaacct tacccgcaga tgcacccaaa aatggtcact ataaagccta tattcttggt
     6061 gaaggtgatg ataacacagc gaaaacgccg cagtgggctt cgcaaattac cggtatcccg
     6121 gtagaccgta tcatcaaact ggcgcgtgaa attggcacag caaaacccgc ttatatctgc
     6181 cagggctggg ggccgcaacg ccaggcaaac ggcgaactga ctgcacgcgc tattgctatg
     6241 ttacctattt tgacgggcaa tgtcggcatc agcggcggaa atagtggcgc gcgtgaatcg
     6301 acctatacca ttaccataga acgcctgccg gtgctggata atccggtcaa aacgtcaatc
     6361 tcctgcttca gctggacaga tgctatcgat catggtccgc aaatgacggc aatccgcgac
     6421 ggcgtccgcg gcaaagataa actggatgtg cccattaagt tcatctggaa ctacgcggga
     6481 aataccctcg ttaatcagca ttctgacatc aacaaaacgc atgaaattct gcaggacgaa
     6541 tcgaaatgcg aaatgattgt gatcatcgaa aactttatga cctcatcggc aaaatatgcc
     6601 gacattctgc tgccagacct gatgaccgtt gagcaggaag atattattcc taacgactac
     6661 gccggtaaca tgggatatct cattttcctc cagcctgtca ccagcgaaaa attcgaacgc
     6721 aaaccgattt actggatcct gagtgaagtc gcgaaacgtc ttggacctga cgtctatcaa
     6781 aagttcacag aaggtcgcac gcaggaacaa tggttacaac atctgtacgc caaaatgctt
     6841 gccaaagatc cggcgttacc gtcttacgac gaactgaaaa aatgggtatc tataagcgta
     6901 aagatcccaa tggccatttt gtcgcctaca aagcatttcg tgacgacccc gaggcaaatc
     6961 cacttaaaac gccttccggt aagattgaaa tttattccag caggctggcg gaaattgccc
     7021 gtacctggga actggaaaaa gatgaagtga taagcccatt gccagtttat gcctcaacct
     7081 ttgaaggctg gaactcccct gaacgtagaa ccttcccact gcaactgttt ggtttccatt
     7141 acaaatcccg tactcactcg acctacggca atattgatct cctgaaggct gcctgccgtc
     7201 aggaggtgtg gatcaaccct atagatgcgc agaaacgtgg gattgccaac ggcgatatgg
     7261 tgcgggtgtt taaccatcgt ggcgaagttc ggctaccagc caaagtaaca ccacgtattc
     7321 tccctggagt tagcgctatg ggccagggag cctggcacga ggccaatatg tctggcgata
     7381 aaatcgacca tggcggctgt gtgaatacgc taaccactct gcgcccttca ccactggcga
     7441 agggaaaccc gcagcacact aatctggtcg agatcgaaaa aatataaccc acgacaacca
     7501 taaattctgg catgacattt tgtttgaaaa gcaataagtg agtaatgatg aaaatccata
     7561 ccacagaggc gctcatgaag gctgaaatca gccgtagaag tctgatgaaa acctccgcac
     7621 ttggcagtct tgcgctggca agcagtgctt tcactctgcc attttcccaa atggtccggg
     7681 cggcagaggc tccggtagaa gagaaagcgg tctggagttc ctgcaccgtt aactgcggga
     7741 gccgctgtct gttacgtttg catgtgaaag atgacaccgt gtactgggtg gagtctgata
     7801 cgacaggtga cgacgtctac ggtaaccatc aggttcgagc gtgtttacgc gggcgctcta
     7861 tccgccgacg gatgaatcat cctgataggt tgaaatatcc catgaagcgc gtcggcaagc
     7921 gcggtgaagg ttaatttgaa cggataagtt gggacgaagc cctggatacc atcagtgata
     7981 atcttcggcg gatcctgaaa gattacggca atgaggctgt acatgtcctg tacggaacag
     8041 gcgtagatgg cggaaacatc accaactcaa acgtcccgta ccgtctgatg aactcttgcg
     8101 gtggttttct cagtcgctat ggcagctaca gtaccgcaca gatcagtgcc gcaatgagtt
     8161 atatgttcgg tgccaatgat ggcaacagcc cggatgatat cgccaatacg aaactggtcg
     8221 ttatgttcgg aaataacccg gcagaaacgc ggatgagcgg cggtggtgtc acttactacg
     8281 tcgagcaagc ccgcgaacgt tcaaacgcac gcatgatcgt catcgatcca cgttataacg
     8341 acactgctgc cgggcgtgaa gatgaatggc tgcccattcg ccctggcacc gatggcgcac
     8401 tggcctgtgc gattgcctgg gtactgatta ctgaaaacat ggtcgatcag ccatttctcg
     8461 acaaatattg tgttggttac gatgaaaaaa cgctgcccgc caacgcacca cgtaacgcgc
     8521 attataaagc ctatattctg ggcgaagggc ctgacggcat agctaaaacg ccggaatggg
     8581 cagcaaaaat caccagcatc ccggcagaaa aaattatcca gttggcacga gagatcggtt
     8641 cagcaaaacc tgcttatatt tgtcagggtt gggggccaca acgacattcc aacggcgagc
     8701 aaacgtcccg cgctattgcc atgctttccg ttctcaccgg taacgtcggc ataaacggcg
     8761 gcaactcagg tgtacgcgaa ggtagctggg atctgggggt agaatggttc ccgatgcttg
     8821 agaatcctgt taaaacgcag atttccgtct ttacatggac agatgccatc gaccatggta
     8881 cggaaatgac cgcgacccgt gatggtgttc gtggaaaaga aaaactggat gtccccatca
     8941 agtttttatg gtgctacgcc agtaacacat tgatcaatca acatggcgac atcaatcaca
     9001 cccatgaggt gcttcaggat gacagcaagt gcgagatgat tgttggcatt gaccacttca
     9061 tgacggcctc ggctaagtat tgcgatatcc tgttgcccga cctgatgcca acagagcaag
     9121 aagaccttat ctctcatgaa tctgcaggga atatgggcta tgtgatcctc gcccaacccg
     9181 caacctcagc aaaatttgaa cgcaaaccca tctactggat gctgagtgaa gtcgccaaac
     9241 gcttaggacc agacgtttat caaaccttta ctgaaggtcg cagtcagcat gaatgggtca
     9301 aatatctcca tgcgaaaacg aaggaacgta accctgagat gcccgactac gaggagatga
     9361 aaacgaccgg gatctttaag aaaaaatgcc cggaagaaca ctacgtcgct ttccgcgcat
     9421 tccgtgaaga tccacaggca aacccgttga aaacaccttc ggggaaaatc gaaatttatt
     9481 ctgaacgact ggcgaagatt gcagatacct gggaattgaa aaaagatgaa attattcatc
     9541 cccttcctgc gtatacccca ggttttgatg gctgggacga tcccctgcgg aaaacctatc
     9601 cactgcagtt aacgggcttc cattacaaag cgcgtaccca ctccagctac ggcaatattg
     9661 atgtgttaca gcaggcctgc ccacaagagg tgtggatcaa ccccattgat gctcaggcac
     9721 gcggtatccg tcatggcgat accgtgcggg tatttaacaa taatggagaa atgctgattg
     9781 ccgcaaaagt gactccgcgt attctgcctg gcgttaccgc catcgggcaa ggtgcgtggc
     9841 ttaaagcgga tatgtttggt gaccgggtcg atcacggcgg cagtatcaat attctgacct
     9901 ctcaccgccc ttcaccgctg gcaaagggaa acccgtcgca cagcaatctt gtccagatcg
     9961 aaaaggttta aggagtagcc catgaccaca caatatggat tttttatcga ttccagccgc
    10021 tgtactggct gtaaaacctg cgaactggcg tgcaaagatt ttaaagatct tggcccggaa
    10081 gtcagtttcc gccgtattta tgaatacgct ggcggcgact ggcaggaaga taatggcgtc
    10141 tggcaccaga acgtgttcgc ttattatctc tccatctcct gtaaccattg cgatgacccc
    10201 gcatgtacaa aagtctgccc cagcggcgca atgcataagc gtgaagatgg ctttgtggtg
    10261 gttgatgaag atgtttgtat cggctgccgc tactgccaca tggcttgtcc gtacggcgca
    10321 ccacagtaca atgctgaaaa agggcacatg acgaagtgcg atggttgtta ttcgcgcgtc
    10381 gccgagggga aacaacccat atgcgtcgaa tcctgcccgc tgcgtgcact tgagttcggt
    10441 ccaattgaag agctgcgcca gaagcacggc actctggctg ccgtcgcgcc actgccgcgc
    10501 gcgcatttca caaaacccaa tatcgttatc aaacctaacg ccaacagccg cccgaccggc
    10561 gataccactg gttatctggc taatccggag gaagtgtaaa atgggaaatg gatggcatga
    10621 atggccactg gttatcttta ccgtattggg tcaatgcgta gtaggcgcgc tgattgttag
    10681 cggaattggc tggttcgccg ccaaaaatga cgcagaccgt cagcgtattg tccgtggcat
    10741 gtttttcctc tggctgttga tgggcatcgg gtttattgcc tctgtcatgc acctgggatc
    10801 gccactgcgg gcgtttaact cgctgaatcg aatcggagct tctggtctga gtaacgagat
    10861 tgctgccggt tccatatttt ttgccgttgg cggactatgg tggctggtgg cggtcatcgg
    10921 taaaatgccg caagcgttgg ggaaactctg gttacttgtc agcatggcgc tcggcgtcat
    10981 tttcgtctgg atgatgacct gcgtgtatca aatcgacact gtgccaacct ggcataacgg
    11041 ttacactacg ctggctttct tcctgacggt attgttgagc ggtccaatac ttgctgccgc
    11101 aattctgcgg gcagcccgcg ttacatttaa taccacacca tttgccatca ttagcgttct
    11161 ggcgctgatc gcttgtgccg gggtgattgt gctgcaaggt ttgtctctgg cgtctattca
    11221 ctcatccgtg caacaagcca gcgcactggt accagattac gcctccttac aggtctggcg
    11281 tgtggtattg ttatgcgcag gtcttggatg ctggctttgc ccgctcattc gtcgccgtga
    11341 acctcacgtt gccggactta ttctggggct aatcctcata cttggcggtg agatgatcgg
    11401 gcgtgtgctc ttttatggcc tgcacatgac agtaggaatg gctattgcag gttaacaaat
    11461 tggtgcgggg caacccgcac atttcaggat gttaggaatg acccattttt cacagcaaga
    11521 taatttttct gtcgcggcgc gcgtgttggg cgcgctgttt tattacgctc cagagagtgc
    11581 ggaagccgca cctctggttg cggtactcac cagtgacggc tgggaaactc agtggcctct
    11641 accagaagcg tcattagcgc cgctggtgac ggcttttcag acacagtgtg aagagactca
    11701 cgcccaggcc tggcagcgtt tgtttgtcgg cccgtgggca ctgccgtctc cgccgtgggg
    11761 gtcggtctgg ctggatcgcg aatctgtgct gtttggcgat tcaacattgg cacttcgtca
    11821 gtggatgcgc gagaaaggca ttcagtttga aatgaagcaa aacgaaccgg aagatcattt
    11881 tggttcactg ctgttgatgg ctgcgtggtt ggcggagaat ggtcgccaga cagaatgcga
    11941 agaattgctg gcatggcacc tttttccgtg gtcaacacgt tttcttgatg tttttatcga
    12001 aaaagcagaa caccctttct accgtgcact gggtgaactg gctcgcttaa cgctggcgca
    12061 atggcaatca caactgttaa ttcctgtcgc ggttaaaccg ctgtttcgat aggatcaccg
    12121 taatattgcc ggatggtgac gattacgcgc aatccggcaa taataggtta cagtgtcacg
    12181 tttttttatc tcttaaagca cgcactgctt ttgcggctgg cctcttttgc cgcaaaatag
    12241 tcgcccgtgt ttcattgccc atttctgctc atgcatcatc tacacatcta tccggatctg
    12301 cgcactatgt tccgccgtct gcttatcgca acagtcgtcg gtattctcgc ggcctttgcc
    12361 gttgccgggt ttcgtcatgc gatgctgcta ctggagtggt tgttcctcaa taatgactcc
    12421 ggcagtctgg tcaatgcagc gacaaacctt tccccctggc gacggttgct aactccggcg
    12481 ctcggcggac tggcggcggg tttgttgctg atgggctggc agaaatttac ccaacaacgc
    12541 cctcatgcgc cgaccgatta catggaagcg ttgcaaaccg atggacagtt cgattacgca
    12601 gcaagcctgg ttaaatcgct tgcctctctg ctggtagtaa ccagcggcag tgcaattggt
    12661 cgcgaaggtg cgatgattct tttagctgcc cttgccgcct cctgttttgc ccaacgtttt
    12721 acgccacgcc aggagtggaa attatggatc gcctgtgggg ccgcggcggg aatggctgcg
    12781 gcctatcgtg ccccgcttgc tggcagttta tttatagccg aagtgctgtt tggcactatg
    12841 atgttggcct ctctcggccc ggtgattatt tccgccgtcg tggcattgct ggttagcaat
    12901 ctgattaatc atagcgacgc gttactctac aacgtacaac tctcagtgac ggttcaggct
    12961 cgtgactatg cgctgattat cagtacaggt gtgctggcag gtctgtgcgg accactgttg
    13021 ttaacgttaa tgaacgcctg tcatcgtgga tttgtgagtc tcaaacttgc gccgccctgg
    13081 caactggcac taggcgggtt gatcgtgggt ctgctttccc tgttcacacc tgcagtgtgg
    13141 ggcaacggct atagcaccgt acaatccttt ttaaccgccc caccactgtt aatgatcatt
    13201 gccgggatct tcctttgtaa actgtgtgcc gtgctggcga gtagcggttc cggcgcaccc
    13261 ggtggtgtct ttacaccgac gctatttatc ggtcttgcca ttggcatgtt gtatggtcgt
    13321 agcctgggat tatggttccc tgatggcgaa gaaattacgc ttttactcgg attgaccggg
    13381 atggcgacac tgttggcggc aaccacgcac gcgccgatta tgtcgacgtt gatgatatgt
    13441 gaaatgaccg gggagtatca gctactcccc ggtttattga ttgcctgcgt cattgcgtcg
    13501 gtaatttcgc ggacgttaca ccgtgactct atctaccgcc agcacactgc gcagcatagc
    13561 taagcggatg tattgcccca gttctcgctg ttcggcgcgc ggcagataag gcagttcacc
    13621 aatgagcggt gccggaagtt ttttacccag cacatcaatg atttccgcat aatgcgccag
    13681 tcctgggttg attcggttag ccacccagcc aatgagcggc agcccgtcgt tggcaatcgc
    13741 ctgagctgtt agcagtgcat ggttaatgca accttcctga ataccgacaa ccatcaacac
    13801 cggcaactgt tcctgcacca cccattcaga gagtggacgc aaatcattca tcagactgcg
    13861 ccagccgcca gtcccttcta ccacgacatg atcgacttta tcggtcaggt ttgccaggcc
    13921 gtttgaaatg agggtgtaat tgattgggca actgtgcgcc acgctacttt cttcttcgct
    13981 taacgcgata ggattaactg cttcataagg cagttcgatg gttgaaacac tctgcaacac
    14041 cagggcatct ttattacgca gcccttcggg tgtctctttg ctccccttcg ctacgggttt
    14101 atatcccgca accgtttttc cctgggaggc taacgcttgt agcaatgcgc gggaaaccac
    14161 cgttttccct acagaagtgt ctgtaccggt aataaagaaa cgcttcagca tcactaactc
    14221 caccgttatg cttcacaaat ataaaccagg aaaataatta accttgaaag tctaagttat
    14281 gctttcctgg cccaaattga gatagcgcaa attttggtag aacagttaaa aaatgttaac
    14341 cctgcaacag acgaatcaac aaagaaccgt tatacatcgc gtcttttacc agtgcagcgc
    14401 ctgccatcgt gccctggtta gaaaactgag tactctcaac gctgatgtgc tgactatacg
    14461 caggaagggc ctgctgacgg atgctgtctg agatgaccgg gaagaggata tctgccgctt
    14521 tacttaacgg tgagccaatc agtatttttt gtgggttaaa taaattcacc atgatggcaa
    14581 gaatgcgccc tacatgcgcg cccaccccgg taatgatgtc ttttgccagc agatcgccgc
    14641 gcaatgccgc ctgacacaat gagtccacgg ttaacggttg tccatgtaac atcgagctca
    14701 tggattgatt aagacgcagc tgtgccagct caagaatact gtccacactg gcgatggttt
    14761 cgaggcagcc gtgattcccg caataacagc gtttcccata cgggtcgacc tgtgtgtggc
    14821 ctatttccac gagactactg ctgcctgcgt gtagcagatg accatcggta atgacgcccg
    14881 cccccacgtt gtgatcgata accacctgaa tcacatcgcg cgccccgcgt gaggcaccaa
    14941 acaaggcctc tgccatcgtc catgcgctga tatcatgctg aatataaacc ggtacgccgg
    15001 tatgctgctc cagcgcctcg ccgagcggca tctcttttac atcctcgtag aacggcatgc
    15061 gatgtacaat accattttcc gtatcaataa ttcccggcaa ggttatggca atcgaagtta
    15121 gacgctcaag ttttttctgg tggcgaataa aaaactgatc gatatgggaa atgatacgat
    15181 ccagcaatgg cgagtcatct tttaacgcca gttcctgcga ctcttccacc accagtttgc
    15241 tgctcagatc gcgcagagca aggaaaatct ccccgcgact aatgcgcaga gaaagatagt
    15301 gccaggcttc agtttcaacc accagcccca ccgccggacg gccacggttc cccgcttctt
    15361 tgatttccag ctcttgcacc aggtgtgctt cgagcatctc acggacaatt ttagtgatac
    15421 tggcaggagc cagctgcgcc agacgggaaa gatcgatacg cgagactgga ccaagctgat
    15481 caatcaggcg ataaaccgcg cccgcgttgg tctgctttat ttgatcaatg tgcccaggct
    15541 ggttttcagc aaccaccgca tactccctat attttcgcgc tccgaaataa tctgtaggct
    15601 atggtgaagc acttcaatac gtgtcgtcaa atttttactt aggcatgtga ttaacagcac
    15661 atttttcggg ctttttcgct gaaatttccc tcacctgaga gcattcagca gatgaatacg
    15721 aaagttacgc gcagccggac tttgttcatg atgtttcggc cagaccaacc acatttcaga
    15781 aaccgcatcc tcttcagcaa tcggcaccca gcgcatttcg ttgagctgaa cacgtttaaa
    15841 tgaagcaggc aaaattgaaa cacccagacc ggcggaaacc agaccgatga tggtcattgc
    15901 ctcgcccacc tcctgagtga tgacgggcgt caaatggtaa cgtcgcatca gcccgagaat
    15961 atcgtcatac agccctgtcc cgacgtgcgg atcaaaaaag acgaagggtt ctttcgccag
    16021 ttcagccagc gttacattcg ggttatttgc caggggatga tcgtgcggga tcatcgccat
    16081 aagcggttca tggacgatga ctgcgtgttc aagcgtctcc ggtaacgctg tgtaacgcag
    16141 caatcccata tccagcgttc cttcaatgag cggagcgatt tgctcacgag tgttcatttc
    16201 gcgggtttgt aaatggacat cgggataatc acggcgaaac agcgataacg tatcggacac
    16261 cgcccgaata aaaggagcgg acgaagtaaa accaatgcgc aactcccccg cttccccctg
    16321 atgcagtctt tcagcgcgag cggcagcgtc atctaccata gacaggattt gccgactatc
    16381 tgcaagaaac tgttttcctg ctgccgtcag caatacactg cgattagttc gtgccagcag
    16441 tcgggcacca atttgttgct ccagtgcctg aatctgctga cttagcggtg gttgcgaaat
    16501 attcaggcgg gcagcggcgc gcccgaaatg cagctcttcc gcaacagcaa caaagtaacg
    16561 cagatgacga agttcaatat tcatatttaa aacatcttat ttgagattat taatatatta
    16621 gacagaacaa ttcgattttc ctaacctatg tataagcctg atccacaggc atatttagca
    16681 aggatttcaa gtgagccgta ctacaactgt tgatggcgct ccggcaagcg acactgacaa
    16741 gcaaagcatt tctcagccaa atcaatttat taaacgcggt acgccgcaat ttatgcgcgt
    16801 caccctggcg ctgttctctg ccggactggc aacatttgca cttctctatt gtgtgcagcc
    16861 tatccttccg gtgctttcgc aggagtttgg cttaacgccc gcgaacagta gtatttcact
    16921 gtccatttcc acggcgatgt tggctattgg tttgctgttt actggccccc tatccgatgc
    16981 cattggtcgc aaaccagtga tggtcacggc gctactgttg gcctccattt gtacgttact
    17041 ttcgacaatg atgaccagct ggcacggcat tttgattatg cgcgccttga ttgggctttc
    17101 gttaagtggc gtggcagctg ttggcatgac ttatcttagc gaggaaatcc accccagttt
    17161 cgtggccttt tcgatggggt tgtatatcag cggcaactca attggcggca tgagcggacg
    17221 cttaattagt ggtgtcttca cggacttttt caactggcga attgctctgg cggcaatcgg
    17281 ttgtttcgcg ctagcctcgg cgttgatgtt ctggaaaatc ctccctgaat cacgccattt
    17341 tcgcccgact tcgctgcgac ctaaaacgtt gtttatcaac tttcgtctgc actggcgtga
    17401 ccggggatta ccgttattgt tcgcagaagg ctttttgctg atggggtcgt tcgtcacgct
    17461 gtttaattac atcggatatc ggttgatgct ctccccctgg catgtcagtc aggccgtggt
    17521 tggcttatta tcgctggctt atttgaccgg tacatggagc tcacccaaag ccggaaccat
    17581 gaccacccgc tacgggcgcg gtccggtgat gttgttttcg acgggggtta tgctgtttgg
    17641 tttactgatg accttattca gctcgctgtg gctgatcttt gccggaatgt tactcttctc
    17701 agcaggattc ttcgcagccc actcagttgc cagcagctgg attggccccc gcgcaaaacg
    17761 cgctaaaggc caggcctcct cgctgtatct gttcagttac tatctggggt cgagtattgc
    17821 cgggacgctg ggtggtgttt tctggcataa ctatggctgg aacggcgtcg gcgcatttat
    17881 tgctctgatg ctggtcattg ctctgctggt cgggacgcgt ttgcatcgtc gtctgcacgc
    17941 ctgaaaaata agcccggact gcggtaacta cacgtccgga cttattgctt gctcaaacca
    18001 acgttaatag ccatcctaaa atagacgaag cgccagccaa ttcccgcagc gcgtctagcg
    18061 tcatcaggat tataagtacc caaataaacg gattcatttt gctgtgtgtc atttattact
    18121 gatgcgcagt tattctaccg ctttgtaagt agtaaaatag ttaacccgat caagactact
    18181 attattggta gctaaatttc ccttaagtca caatacgtta ttatcaacgc tgtaatttat
    18241 tcagcgtttg tacatatcgt tacacgctga aaccaaccac tcacggaagt ctgccattcc
    18301 cagggatata gttatttcaa cggccccgca gtggggttaa atgaaaaaac aaattgaggg
    18361 tatgacaatg aaaaaagtat tagctctggt tgttgccgct gctatgggtc tgtcttctgc
    18421 cgcctttgct gcagagactg cgaccacacc tgctccgact gcgacgacca ccaaagcagc
    18481 gccggcgaaa acgacacatc ataaaaaaca gcataaagca gcacctgccc agaaagcgca
    18541 ggcggctaaa aagcatcata aaaatacgaa agctgaacag aaagcccctg aacaaaaagc
    18601 gcaggcagcg aagaaacacg ccgggaaaca cagccatcag caaccggcaa aacctgctgc
    18661 acaacccgca gcgtaagttt tcaacagtaa tgctggcgcg ccccctcgcg cctgaaaatt
    18721 acggtgctaa gcaggtaacg tttagcaccg cctttagcgg agggtagtat ggtgggccgt
    18781 tatcgctttg agttcattct tatcatcctt attttatgcg cactgattgc cgcccgtttt
    18841 tatctttcct gactgtagtt atctgatttt actcccactt tcatcccgtc ccgtctatag
    18901 tatttatgag ggtttgcttt taataatcat aattacccac cagagtgtga tatgcgtaca
    18961 accattgctg tagtgttggg tgcaattagt ttgacgtctg cttttgtgtt tgcagataaa
    19021 ccagacgttg ccaaatcggc aaacgatgag gtcagcaccc tgttttttgg tcatgatgat
    19081 cgtgtgccag tgaatgacac gacccaatca ccgtgggatg cggttgggca actggaaacg
    19141 gccagcggca atttatgtac ggcgacgctg attgcaccca atctggcatt aacggcagga
    19201 cactgtttat tgacgccgcc taagggtaaa gcggataagg cggtagcgct gcgttttgtg
    19261 tcaaataaag gtctttggcg ctatgacatc cacgacatag aaggccgcgt tgatccgaca
    19321 ctgggaaagc ggttaaaagc agatggggat ggttggattg ttcctcccgc agctgcacca
    19381 tgggacttcg gcttgattgt gctgcgtaat cccccttctg gcattacacc gttgccgtta
    19441 tttgagggag ataaagccgc gcttactgcc gcattaaaat cggctggtcg taaagtgact
    19501 caggcgggat atcctgaaga tcatctcgat acgttataca gtcatcaaaa ctgtgaagtg
    19561 actggctggg cgcaaacgtc ggtgatgtca catcagtgcg ataccttgcc gggtgacagc
    19621 ggttcgcctt tgatgttgca taccgatgac ggctggcaat taattggggt gcaaagttcg
    19681 gctcctgccg cgaaagatcg ctggcgcgcc gataaccggg ccatttctgt taccggtttt
    19741 cgcgacaagc tggatcaact gtcgcaaaaa taatgttcaa acgctgcccg acagtgcggg
    19801 cagcgtgttc atcaggcaag tttcaccatg atcattccag ccaacagcaa gaccaggcca
    19861 atccagcctt tacgatttaa ccgttgacca aacaagatcc aacctgcggc taacgtggcg
    19921 gcaataccaa acccgcccca caatgcataa gcgacagaca agtcgatccc ttttacggcc
    19981 tgagaaagcg cactaaaggc agccagcacc gccgccaggg agagcaagcc aaatattttg
    20041 cgacgaaagc cgtcagaaaa tttcaaaaag acgttagcaa cgatttccag cacgattgcc
    20101 aatgccagcc aggcggcgtg aacccattca aactgcgcca tggttcactt ccagttcagg
    20161 tttacgcgct ttacgggtac ctgatttgat caacacaatc ccggcgacca gggtggttaa
    20221 cccggcaatt ttcatcagcg ataaactttc gtcgaataac aaaacgctaa acaaggtaat
    20281 aaataaaata ccgatacctt cccacagcgc ataagctacg cctaaggcga tctttttaac
    20341 ggcgaaagag agaaatatat acgacagaga aatcatcacc agcattaaaa taaagccgcc
    20401 atttccctca ctgacgctcg cccatttcat tga
//
LOCUS       DAZGJW010000081        19849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZGJW010000081 DAZGJW010000000
VERSION     DAZGJW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.081"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(94..375)
                     /gene="yehE"
                     /locus_tag="ACHKIK_002982"
     CDS             complement(94..375)
                     /gene="yehE"
                     /locus_tag="ACHKIK_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO9084116.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(638..1747)
                     /gene="apbC"
                     /locus_tag="ACHKIK_002983"
     CDS             complement(638..1747)
                     /gene="apbC"
                     /locus_tag="ACHKIK_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO9084117.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1879..3912
                     /gene="metG"
                     /locus_tag="ACHKIK_002984"
     CDS             1879..3912
                     /gene="metG"
                     /locus_tag="ACHKIK_002984"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO9084118.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4053..7847
                     /locus_tag="ACHKIK_002985"
                     /pseudo
     CDS             4053..7847
                     /locus_tag="ACHKIK_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7857..11489
                     /gene="yehI"
                     /locus_tag="ACHKIK_002986"
     CDS             7857..11489
                     /gene="yehI"
                     /locus_tag="ACHKIK_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO9084119.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVIARQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFNQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVHRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11551..11868
                     /gene="yehK"
                     /locus_tag="ACHKIK_002987"
     CDS             11551..11868
                     /gene="yehK"
                     /locus_tag="ACHKIK_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO9084120.1"
                     /translation="MIVQKELVAIYDYEIPVPENPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDTDPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            13108..14196
                     /gene="yehL"
                     /locus_tag="ACHKIK_002988"
     CDS             13108..14196
                     /gene="yehL"
                     /locus_tag="ACHKIK_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO9084121.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            14207..16486
                     /gene="yehM"
                     /locus_tag="ACHKIK_002989"
     CDS             14207..16486
                     /gene="yehM"
                     /locus_tag="ACHKIK_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO9084122.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIKGPADFNDRV
                     DELFLSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEANMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            16479..17615
                     /gene="yehP"
                     /locus_tag="ACHKIK_002990"
     CDS             16479..17615
                     /gene="yehP"
                     /locus_tag="ACHKIK_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO9084123.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            17612..19612
                     /gene="yehQ"
                     /locus_tag="ACHKIK_002991"
     CDS             17612..19612
                     /gene="yehQ"
                     /locus_tag="ACHKIK_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO9084124.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEGLATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            19737..>19849
                     /locus_tag="ACHKIK_002992"
     CDS             19737..>19849
                     /locus_tag="ACHKIK_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084125.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNG"
BASE COUNT         4965 a         5126 c         5033 g         4725 t
ORIGIN      
        1 tgtaaatggt attgttgata tcaataaaaa agccgaaatt atttatataa atgatttcgg
       61 ctttctaaat gcaattaaaa tcaggttgcc aaattaatcg tagcgattta acacgattcg
      121 acgtttgcta tcgctacctg caacaaccac ttctgtcgtt acacctttga cgggcgaaga
      181 aaacagatga cgggtgtcaa cattatacgt taactgacca caatgctgtt gaggttgacc
      241 attaaccata gccatactgc atgttggggg actgatcctg ccattaatcg ctaatgtggc
      301 agtctctgaa gagaatgcgg gagaagccaa tccataggca agaaaaataa ttcctgacag
      361 ccaatactta ttcattgaac gttatccctg tagtaaaggt tatgcctgga tagaatgagt
      421 gcataacaaa ctatagctgt acatccacta cacagccacg aaggatgata atgaagcatt
      481 gcctgtatga tcaatcgact ttgtagagtt tcggacgaag gtccgcagaa tattcgcagt
      541 attaaataag tcttcattaa tgcggcaaaa actaatacac cgcatcaatg taacatctct
      601 gcggtgtatg aagaataacc agaatggtta ataggcgtta gaccgcgcgg aaggaaatct
      661 cgcctggaat gacttcaccc tgccagtaga gctgagctgc aacgcggtca gccagctggc
      721 ggtagatagc ggtaaattcg ctctccggac ggctgataac cgttggcgtg cctttatcga
      781 gatcctcacg taaggagatg tgtaacggca tctgccccag cagctgtgtg tggtattttt
      841 cagccagttt ctcagcacca ccggtaccga aaatcggttc atggtgacca cagttactgc
      901 aaatatgcac actcatgttc tcgacaatac caagcaccgg aacctcgact ttctcgaaca
      961 tcacaatgcc ttttttcgca tcgatcaacg cgatgtcctg cggggtggtg accacgactg
     1021 caccagttac tggaatgttt tgcgccagcg tcaattgaat gtcaccggtg cctggcggca
     1081 tatcaagcac cagatagtcg agatccggcc acaaggtttc ctgcaacatc tgcattaacg
     1141 ccttgctcgc catcggtcca cgccacacca tcgcattgtc gtcagtgacc agataaccaa
     1201 ttgaattggt tgccaggcca tgagacataa tcggtgccat gtgggtaccg tccggcgacg
     1261 ttggacgctg attttctgcg cccagcatgg tgggaattga tgggccgtag atatcggcgt
     1321 ccagaatgcc gactttcgcc ccttccgccg ccagtgccag cgccaggttt accgccgtgg
     1381 aggatttacc cacgccgcct ttaccggaac tgaccgcaat gatgttcttc acaccattaa
     1441 tacccggctg attcttcacg cgttttaagg tggcgatatt gtgcgacagt ttccagtcga
     1501 tagctttcgc cccggtgata cgcagcagtt cggcgctaca ttgctctttt aactcttcaa
     1561 acgcgctatg ccagacgaat ggcataacca gttccacatg caacgtgtcg tccatccagg
     1621 cgacatggtg caacgctttc agcgtggtga ggttatgctt cagggttggg tgctgaaaat
     1681 tggccagtgt cccggcgacc attgcgcgca aggcttctgg tgatttggcc tgggattgtt
     1741 cgttcatccc gactcctttt ttgtatagat aaaccatcag ctgatagttt acctgaagaa
     1801 tatagagaag tacttactta acattttccc atttggtact atctaacccc ttttcactat
     1861 taagaagtaa tgcctactat gactcaagtc gcgaagaaaa ttctggtgac gtgcgcactg
     1921 ccgtacgcta acggctcaat ccacctcggc catatgctgg agcacatcca ggctgatgtc
     1981 tgggtccgtt accagcgaat gcgcggccac gaggtcaact tcatctgcgc cgacgatgcc
     2041 cacggtacac cgatcatgct gaaagctcag cagctcggta tcaccccgga gcagatgatt
     2101 ggcgaaatga gtcaggagca tcagactgat ttcgcaggct ttaacatcag ctatgacaac
     2161 tatcactcga cgcacagcga agagaaccgc cagttgtcag aacttatcta ctctcgcctg
     2221 aaagaaaacg gttttattaa aaaccgcacc atctctcagc tgtacgatcc ggaaaaaggc
     2281 atgttcctgc cggaccgttt tgtgaaaggc acctgcccga aatgtaaatc cccggatcaa
     2341 tacggcgata actgcgaagt ctgcggcgcg acctacagcc cgactgaatt gatcgagccg
     2401 aaatcggtgg tttctggcgc tacgccggta atgcgtgatt ctgaacactt cttctttgat
     2461 ctgccctctt tcagcgaaat gttgcaggca tggacccgca gcggtgcgtt gcaggagcag
     2521 gtggcaaata aaatgcagga gtggtttgaa tccggcctgc aacagtggga tatctcccgc
     2581 gacgcgccat actttggttt tgaaattccg aacgcgccgg gcaaatattt ctacgtctgg
     2641 ctggacgcac cgattggcta catgggttct ttcaagaatc tgtgcgacaa gcgcggcgac
     2701 agcgtaagct tcgatgaata ctggaagaaa gactccaccg ccgagctgta ccacttcatc
     2761 ggtaaagata ttgtttactt ccacagcctg ttctggcctg ccatgctgga aggcagcaac
     2821 ttccgcaagc cgaccaacct gtttgttcat ggctatgtga cggtgaacgg cgcaaagatg
     2881 tccaagtctc gcggcacctt tattaaagcc agcacctggc tgaatcattt tgacgctgac
     2941 agcctgcgtt actactacac tgcgaaactc tcttcgcgca ttgatgatat cgatctcaac
     3001 ctggaggatt tcgttcagcg tgtgaatgcc gatatcgtga acaaagtggt taacctggcc
     3061 tctcgtaatg cgggctttat caacaagcgt tttgacggcg tgctggcaag cgaactggct
     3121 gacccgcagt tgtacaaaac cttcactgat gccgctgaag tgattggtga agcgtgggaa
     3181 agccgcgaat ttggtaaagc agtgcgcgaa atcatggcgc tggctgatct ggctaaccgc
     3241 tatgtcgacg agcaggctcc gtgggtggtg gcaaaacagg aaggccgcga tgccgacctg
     3301 caggcaattt gctcaatggg cataaacctg ttccgcgtgc tgatgactta cctgaagccg
     3361 gtactgccga aactgaccga gcgcgcagaa gcattcctca atacagaact gacctgggat
     3421 ggtatccagc aaccgctgct gggccacaaa gtgaatccgt tcaaagcgct gtataaccgc
     3481 atcgatatga agcaggttga agcactggtg gaagcgtcta aagaagaagt gaaagcagct
     3541 gccgcgccag ttactggccc actggcagat gatccgattc aggaaaccat cacctttgac
     3601 gacttcgcaa aagttgacct gcgcgtggcg ctgatcgaaa acgcagagtt tgttgaaggt
     3661 tctgacaaac tgctgcgcct gacactggat ctcggcggtg aaaaacgcaa tgtcttctcc
     3721 ggtattcgtt ctgcctaccc ggatccgcag gcactgatcg ggcgtcacac cattatggtg
     3781 gccaacctgg caccgcgtaa aatgcgcttt ggtatctccg aaggcatggt aatggctgct
     3841 ggtcctggcg ggaaagatat tttcctgcta agcccggatg ccggtgctaa accgggtcat
     3901 caggtgaaat aatccccctt caaggcgctg catcgacagc gccttttctt tataaattcc
     3961 taaagttgtt ttcttgcgat tttgtctctc tctaacccgc ataaatactg gtagcatcag
     4021 cattcaactg gataaaatta cagggatgca gaatgaaaca ctttatctat caggacgaaa
     4081 aatcacataa attctgggca gttgagcaac agggaaacga gttgcatatc agttggggaa
     4141 aagttggcac caaagggcaa agccagataa aaagtttttc agatgctgcg gcagcggcaa
     4201 aagcggagct taagctgatt gcggagaagg tgaagaaggg gtatgtggag caagcgaagg
     4261 ataattcttt gcaaccttcc caaacggtaa cgggctctct caaggtagcg gacttatcca
     4321 ccattattca ggaacaaccc tctttcgtag cagaaacccg tgcgcctgac aaaaatacag
     4381 atgctgtttt accgtggctg gcgaaagata ttgctgtcgt ttttccgccc gaagttgtac
     4441 acaccacgtt aagtcatcgc cgctttcccg gagttcctgt tcagcaagca gacaaattga
     4501 cccaattacg tcgcttagcc tgtagtgtgt cgcaacggga taataaaaca gccacatttg
     4561 acttcagcgc ctgttcttta gaatggcaaa acaccgtcgc ccaggcgatc agtcagatcg
     4621 acggcctgaa aacaacacag ctaccatcac cagtaatggc tgtactcacg gcacttgaaa
     4681 tgaaatgcac aagatataaa gtgcgtgagg atgttatgga tcagatcgtc caggaaggcg
     4741 gtctggaata tgctactgat gtaataatac accttcaaca gattgatatt aaatgggatt
     4801 atgcgaataa tgtcattatt attctgccgt ctggcattgc acctgactac ttggagcaat
     4861 attccagatt tgaattacgc ctacgtaaac atttatcact ggcggaagag tctctctggc
     4921 aaaaatgtgc acaaaaactt attgccgcaa ttccacatat tccagaatga cggcaaccat
     4981 taattgcttt gttattacct gaaaaaccag aaattgcaca tgaaattgcc cagcgtctac
     5041 tggggcaaaa aaaattaccc tcgcttgagt ggttaaaaat agtggcgact gatgagcaca
     5101 ttcttgcctc attagaaaaa tatcacgagc catatgccat ttttgatgat tactattgtg
     5161 gtgcgatatg gtcagccacc gtattacagg agcaaggtgt tgcagccctg ccccgatttg
     5221 ctccctatgc cgcaagtgac tactgcgccg atgtgttgcg tcatatcaat catccgttcg
     5281 cattaacact gcttatacgt gtagccgggc atactaaacg ctgtcacgat cggatgacga
     5341 aagccattgc tgcgttccca catgcagcaa tggcggcact gacggaactt cttgggcaaa
     5401 aagaagagaa cagttggcgc attatgctaa tgacaatgct tatctcacaa ccagcactgg
     5461 cagaacaggt cattccctgg ctctcgacac ccgcagttgc cgtactgaaa tcatgccagc
     5521 aacaactgac acagccctca aaccatgcca gcgccgatct actgccagcc gtagtagtct
     5581 cccctccctg gctttcgaaa aagaaaaaat cgccgattcc ggtgctggat ttagcgccat
     5641 taggcattga gccaatctgt tatctgacag aagaaatcag taatcaactt ttggcgaaat
     5701 atatctggta ttcaaaacac atcacggtta gccatgaaga aagtactacc aacctgttgg
     5761 caaggatggg ttttcaacga cggatcgctg gtacatatat taaagctccc gaagcggtag
     5821 ttgaggcatg gctaaatgaa gattattcaa ccttactaag tgaatttaag gtgtttcatt
     5881 cacctaccgg gcattattgg cagttgggga ttttgacaac attgccgctg gagaaagcag
     5941 taaaagcatg gaatgccctt accctatctc cacataccga taccgaatac gccatgttac
     6001 attttggact caaagggtta cctgggttag taaactcact tgcacgctat ccacaagaag
     6061 ccttgcccat cacgaattac ttcgcagcga gtgagctggc tcctgccgtc gcccgtgcct
     6121 tcaacaaact gaaaacgcta cgcgaaaacg cccgtagctg gctgttgaaa tacccggaac
     6181 atgcccttac cggcctgctg cctgcggcgc tcggcaaagc cggtgaagca caggataacg
     6241 cccgcgctgc cttgcgtatg cttaccgaaa acggtcatca gccattactg caagaaatcg
     6301 cccgacgtta taaccagccg gaagtaaccg atgcggtgaa cgctctgctt gcgctcgatc
     6361 ccttagataa tcacccgaca aaaatcccca ctcttccggc cttttatcag ccatcgctct
     6421 ggacgcgccc ggtattaaaa gcaaatgccc aatcactgcc agatagcgcc ctcctccacc
     6481 tcggtgaaat gctccgcttc cctcaggaag aggctctgta tccgggatta ttgcaggtga
     6541 aagacgtctg ttccgccgac tcactggcgg gatttgcctg ggatctgttt accgcctggc
     6601 agaccgctgg cgcgccgtcg aaagagagtt gggcgttcac tgcgttaggc gttctcggta
     6661 acgatgacac cgcccgcaaa ctgacgccat taatacgcgc ctggcctggt gaatcccagc
     6721 ataaacgcgc caccgttggg ttggatattc tcgctgctat cggtagtgat atcgccctta
     6781 tgcagcttaa cggcatcgcc cagaaactga aattcaaagc attacaggag cgggcaaaag
     6841 aaaaaattgc cgacattgcc gagagccgcg aactcacggt ggcggagctt gaagatcggt
     6901 tagcaccgga tctcggtctg gatgataacg gttcgctgct gctggatttt ggcccacggc
     6961 agttcaccgt cagctttgat gaaaccttaa aaccgtttgt gcgtgatgtt tccggcagcc
     7021 gcctgaaaga cctgcccaaa ccgaacaaaa gcgatgatga atcgcggtcg aacgatgcgg
     7081 ttaaccgcta caaattgctg aaaaaagatg cgcgtaccgt cgccgcccag caggtagcaa
     7141 ggctggaatc cgccatgtgc ctgcgccgcc gctggtcgcc ggaaaacttc cagctcttcc
     7201 tggttgagca tccgctggtt cgccacttaa cccgccgtct gatttggggc gtttatagcg
     7261 ccgaaaacca gctactggct tgctttcgcg tagcagaaga taacagctac agcaccgctg
     7321 acgatgatct tttcaccctg ccggaaggcg atatctctat cggcattcct cacgttctgg
     7381 agatatcacc gacggatgcc gccgcctttg ggcagctttt tgccgactat gaactgttac
     7441 cgccgttccg ccagctcgac cgtaacagtt acgccctgac agaaaccgag cgcaatgcca
     7501 gcgaattgac ccgctgggca ggcagaaaat gcccgagcgg tcgggtcatg ggactggcga
     7561 ataaaggttg ggtgcgtggt gaaccgcagg atggtggctg gatcggctgg atgatcaaac
     7621 ctctgggccg ctggtcgtta atcatggaaa tcgatgaagg ctttgcagtt ggtatgtcgc
     7681 cagccgaact tagcgccgag caactcttaa gcaagctgtg gctatgggaa ggcaaagcag
     7741 aaagctatgg ctgggggagt aattcaacac aggaagcgca gttctccgta ctcgatgcca
     7801 tcaccgccag cgagctaatt aacgatattg aagccctgtt tgaataagga aagagcatgg
     7861 acaaggaatt accgtggctg gcggataacg cccaactgga actgaaatat aaaaaaggca
     7921 aaacgccgct cagtcatcgt cgctggccgg gcgaaccagt gtccgttatc actggaagtc
     7981 tcatccagac attgggagat gaattactac aacaagcagg ccagaaggaa aatatcacct
     8041 ggaattatga taaatgctca ctggagtggc agtccgccat ccagcaggca atcaatttga
     8101 ctggcgagca taaaccgtca atcccagccc ttaccatggc ggctctgatc tgtatcgcgc
     8161 aaaatgacag ccagcagtta cttgatgaaa tcgtgcaaca agaggggctg gaatatgcga
     8221 cggatgtagt gatagcgcgt cagtgcattg cgcgacgtta tgagagtgac tcactggtcg
     8281 tcactttgca gtatcaggac gaggactatg gctacggtta tggctccgcg acctataacg
     8341 atttcgatct ccgtttgcgt aagcatctgt cgctggcaga ggaaagttgc tggcagcgtt
     8401 gtgccgataa actcattgcc gcactgccag gaatacccaa aattcgccgt ccttttattg
     8461 cgctgatcct cccggaaaaa cctgaaatag ccaatgaact cgccagtctt gaaagctctc
     8521 ggtcaagtct ccattcaaag gagtggttaa aggtcgttgc tactgataat acggcggtaa
     8581 aaaaactcga acgatactgg ggcctggacg tatttagcga tcgcgaagcc agctacatgt
     8641 cacaagaaaa ccgcttcggc tacgcggcct gcgcctccct tttgcgcgaa caaggactgg
     8701 cagccgttcc gcgcctggca atgtatgccc ataaagaaga ttgcggcagt ctgctggtac
     8761 aaattaacca tccgcaagtc atccgcacct tgctactggt ggctgataaa aacaaaccca
     8821 gcctgcaacg tgtggctaaa tacagtaaaa acttccccca cgcgacgctc gccgcactgg
     8881 cagaactgct ggcgttaaaa gaaccaccag cccgcccagg ttatccaatc atcgaagaca
     8941 aaaagctgcc tgcacagcaa aaagcacgcg atgaatactg gcgtacgctg ttacaaacgc
     9001 tgatggcatc gcagccacaa ctggcagcag aagtgatgcc gtggttaagt actcaagcca
     9061 gggcagtgct gaatagttat ttatcggcac cgccaaaacc ggttattgat agcaccgata
     9121 acagcagtct gcctgaaatg ctcgtttcac caccgtggcg tagtaagaaa aaaatgacag
     9181 ctccacgtct tgatttggca ccgctcgaat taactccgca aatttactgg caaccaggcg
     9241 aacaagagag gcttgccgcc actgagtctg cccgttattt cagcacggaa tctcttgcgg
     9301 aacgcatgga acaaaaaagt ggacgagttg tattacagga actgggtttt ggggatgatg
     9361 tatggctgtt tctgaattat atactccccg gaaaactgga tgctgcacgc aattcactca
     9421 ttgttcagtg gcattactac caggggcggg ttgaagagat cctgaatggc tggaactccc
     9481 cgcaagcaca attagcagaa caggcgctcc gcagcggtca catagaagcg ttaattaaca
     9541 tatgggaaaa tgacaacttc tcacgttatc gtccggaaaa gagtgtctgg aacctgtatt
     9601 tattggcaca gttgccgcgt gagatggctt tgaccttctg gctgcgtatc attgagaaaa
     9661 agcatctgtt cgcgggtgag gactattttc tcagtatcct cggattggat gcgctaccag
     9721 gtctgctgtt ggctttttca catcgtccaa aagaaacatt tccgttaatt ttaaattttg
     9781 gcgcaacaga actggcgctg cctgttgccc gcgtctggcg acgtttcgcg gcgcagcgtg
     9841 atctggctcg ccagtggatt ttacaatggc cggaacatac ggctagtgca cttatccctc
     9901 ttgtctttac caaacccagc gataatagcg aagccgcatt acttgccctg cgtttactgt
     9961 acgaacaggg acatggcgaa ttgctacaaa ccgtggcaaa ccgctggcag cgtacagatg
    10021 tatggtctgc cctggagcag ttgcttaaac agggtccaat ggacatttac ccggcacgca
    10081 ttccaaaagc ccctgatttc tggcatccgg caatgtggtc caggcctcga cttatcacta
    10141 ataatcagcc tgttaccggt gacgctctgg aaattatcgg cgaaatgctg cgctttaccc
    10201 aggggggacg tttttatagc gggctggaac aactgaaaac gttctgccag ccacaaacgc
    10261 tggcagcttt tgcctgggat ctcttcactg cgtggcaaca agctggtgcc cccgcaaaag
    10321 acaactgggc atttctggcg ttaagtctct ttggtgacga aagcacggca cgggatctga
    10381 cgacacagat cctcgcctgg ccacaagaag gcaaatctgc ccgtgctgtc agcggcctga
    10441 acatccttac cctgatgaat aatgatatgg cgctgataca gctgcatcat atatcgcaac
    10501 gggctaaatc ccgcccctta cgtgataacg cggcggaatt tcttcaggta gtcgcagaaa
    10561 atcgcgggct aagccaggaa gagctagcgg acagattagt cccaaccctg ggccttgatg
    10621 atccgcaggc gttgagtttt gattttggtc cccggcagtt taccgttcgc ttcgatgaaa
    10681 acctcaaccc ggttatcttt aatcagcaaa acgttcgcca gaaaagcgtt ccccggttgc
    10741 gcgccgatga cgatcaactg aaagcgcccg aggcactggc ccgactaaaa gggctaaaaa
    10801 aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct tgaagctgcc ctacgtacca
    10861 cccgacgctg gtcgctggca gattttcatt ctctgtttgt taatcatccc tttacccgtc
    10921 tggttaccca gcgattaata tggggggttt atccggcaaa tgaaccgcgt tgtttactca
    10981 aagcctttcg tgtggccgca gagggggagt tctgcaatgc gcaagatgag ccaattgacc
    11041 tgcctgcgga cgctctgatt ggcattgccc acccgttaga aatgacagta gaaatgcgca
    11101 gtgaatttgc acagcttttt gccgattacg aaattatgcc gccttttcgc cagttgtcgc
    11161 gccgcacggt gctgctcaca cctgacgagt caaccagtaa cagcctgact cgctgggaag
    11221 gtaaatccgc taccgttggg cagcttatgg gaatgcgata caaaggctgg gagtcaggtt
    11281 atgaggacgc atttgtctat gacctgggtg agtaccggct ggtccttaag ttttcacccg
    11341 gttttaacca ctacaatgtt gatagcaaag cgctaatgag cttccgttct cttcgagtgc
    11401 accgtgacaa taaatccgtc acttttgccg aacttgatgt gtttgatttg agtgaggcgt
    11461 taagcgcacc tgacgtcatt ttccattaaa aacacagcgg gcagtgatgc aactgcccgt
    11521 tatcaacacg acttaccagc ggggaaagcg atgatcgtgc aaaaagagct ggttgctatt
    11581 tacgattatg agatccctgt acctgaaaat ccgttttcct tcagacttga gatccataaa
    11641 tgctctgaat tatttacagg ttccgtctat cgactggagc gattccggct acgtccaaca
    11701 tttcatcaac gtgatcgaga agatactgac ccgctaataa atgatgcgtt gatttatata
    11761 agagatgagt gtattgatga gcggaaatta cgaggtgaat cacctgaaac tgtaatagca
    11821 atttttaatc gtgaactaca gaatatattc aaccaagaaa tagaataata tactctagat
    11881 aattaaagtc gcagcacatg caacttgaag tatgacgagt atagctttct taaaggggga
    11941 aacggtattc ccccttagat gcgtttcaat ttgcccgtta cttcaacaaa tcaatcaacc
    12001 gtaacagagc cactaataac tccagtacgg caatgagcac ggttaacttt tgtgtcagcg
    12061 tgtccatccg ctctccttat taaggagcgc aacaccagca ccttaccctt acactgcctg
    12121 ttgatactgg tttacgtatg ttggtacgaa acgttgctct ccggatcagg gcaccgcctg
    12181 cggcaccacc cgtccggcag ccaggactta aaattggtcc gatccggcac aacctcccaa
    12241 cgggcaggat tataaatctc actcactgaa ttgcaatgga gtgtggatat ggttataatt
    12301 tattcgttgg tacgaaatgt tgcacaatca gcaagttagc gtcactgtag taaataaaag
    12361 aacctgcctc accagcaggt ttttttattt tctgcgatct actttccaga tattttttgc
    12421 caatagccca acataaatat tcacatcccg gtcaaatggt gcgttgaata taaaatcgat
    12481 gaatttataa ataaaaatga gttatatgat gaattaataa aaacagtaat cattttatta
    12541 aagggggaag cggttttccc ccttagatgc gtttcaattt gcccgttact tcaacaaatc
    12601 aatcaaccgt aacagagcca ctaatagctc cagtacagca atgagcacgg ttaacttttg
    12661 tgtcagcgag tccatccgct ctccttatta aggagcgcaa caccagcacc ttacccttac
    12721 actgcctgtc gatactggtt tacgtatgtt ggaccacgat gttgcactcc ggatcagggc
    12781 accacctgca gcaccccccg tccggtagcc aggacttgaa ataggttcga tccggcgcaa
    12841 cgtcccaaca gtcaggatta taaatctcat tatcttaatt gcaacggggt ccagccgtgg
    12901 ttataattcg ttcgttggaa cacgatgttg cacaggctgt ggtgttaggc ctgaaaatag
    12961 taaataaaag aacctgcctc accagcaggt ttttttattt actgtgatct gctttccaga
    13021 tattttccgc tcaaacaact aatgcgccaa acatttattg cgcgtaaaat atcgtttatt
    13081 tcattaatac atttcaggga tgaatatatg tcaccgcaga ataatcatct gcagcgtccg
    13141 cctgctgctg tgttatacgc cgatgaactg gcaaaattaa aacaaaatga taacgcacct
    13201 tgcccgcccg gttggcagtt aagtttgcct gcggcccgtg cttttatcct tggcgatagc
    13261 gcgcaaaata ttagccgtaa agtggtgatt agtccctccg ctgtcgaacg tatgttagtc
    13321 actcttgcca ccggacgcgg tttgatgttg gtgggggaac ctggcaccgc aaaatctctc
    13381 ctgtctgaat tactggcaac cgctattagc ggcgatgccg ggttaaccat ccagggcggc
    13441 gcatctacca ctgaagatca aatcaagtac ggctggaact acgctctgct tatcaatcat
    13501 ggcccatcaa cggaagcgct ggtccctgcc ccgctttacc agggaatgcg cgacggcaaa
    13561 atcgtccgtt tcgaagagat aacgcgtaca ccgctggaag tgcaagattg tctgttaggt
    13621 atgctttctg acagagttat gacggtgccg gaactcactg gtgaagccag tcaactctat
    13681 gcgcgggaag gctttaatat tattgccact gccaataccc gcgatcgcgg cgtcaatgag
    13741 atgagtgccg cgctaaagcg tcgcttcgat tttgaaactg tctttccgat tatggatttc
    13801 gctcaggagc tggagcttgt cgccagtgct tcggcgcgtt tgctggccca tagcggtatt
    13861 ccgcataaag tgcctgacgc ggtactggag ttactggtcc gcaccttccg cgatctgcgt
    13921 gccaacggcg aaaagaaaac gtcaatggat acactaacag cgattatgtc caccgctgaa
    13981 gccgtgaacg tggcccacgc cgtaggcgtc cgcgcctggt ttttagcgaa tcgcgcaggt
    14041 gagccagccg acctggtgga ctgcattgcc ggaaccatcg tcaaagataa cgaagaagat
    14101 cgcgctcgtc tgcgccgtta cttcgaacaa cgagtcgcca cacataaaga agctcactgg
    14161 caggcttatt atcaagcccg ccaccgcctg ccgtgaggaa agatgcatga gcgagccgtt
    14221 aattgtcggc atccggcatc atagtccggc ctgcgcccgg ctggtgaaat cgttaatcga
    14281 aagccagcgg ccacgatacg tgttgattaa aggcccggct gattttaatg accgggtaga
    14341 cgaactgttt ttatcccacc agcttccggt agctatttac agttattgcc agtatcagga
    14401 cggtgcagcc cccgggcgtg gtgcctggac gccatttgct gaattttcgc cggagtggca
    14461 ggcgctacaa gccgcacgtc gtattcaggc acaaacttac ttcatcgatt tgccttgctg
    14521 ggcgcagagt gaagaagagg acgattcgcc tgatacgcaa gatgaaagcc aggccttact
    14581 gctgcgtgcc acccgcatgg ataacagcga taccctgtgg gatcacttgt tcgaagatga
    14641 aagccagcaa actgcattac cctctgcgct ggcgcactat tttgcccaac tgcggggcga
    14701 tttccccggc gatgcactca atcgtcagcg cgaagccttt atggctcgct ggattgcatg
    14761 ggcggtgcag caaaataatg gcgacgtgtt agtcgtctgc ggtggctggc acgctccggc
    14821 actggcaaaa atgtggcgcg aatgcccgca ggacattaac acgccggaat tgccctcgct
    14881 ggcagatgcc attacaggtt gttatctcac gccctacagt gaaaagcgcc ttgatgtgct
    14941 ggcaggatac ctttccggta tgcctgcccc ggtctggcaa aactggtgct ggcagtgggg
    15001 cttacagcag gccggtgaac aactgctaaa aactatcctt acccgtttgc gccagcacaa
    15061 gttgcctgct tcgaccgcgg atatggctgc cgctcatctg catgcgatgg cgctggcaca
    15121 gttgcgcggt catacactac cgttacgcac tgactggctg gatgccatag caggctcgct
    15181 gattaaagaa gccctgaatg cgccgttgcc gtggagctat cgcggcgtta ttcatcccga
    15241 taccgatccg attctgctaa cgttgataga cacattagcg ggtgacggat tcggtaaact
    15301 tgccccttct acgccacaac cgcctctgcc aaaagatgtc acctgcgaac tggaacgtac
    15361 cgcaatctct cttccggcgg agcttacctt aaatcgcttt aaccccaatg gactagcgca
    15421 aagtcaggtg ttacatcggc tggcaatact ggagatccca gggattgtac gccagcaggg
    15481 aagcacactg acacttgcag gcaacggtga agaacgctgg aaattaaccc gcccgcttag
    15541 ccagcatgcg gcattgattg aggccgcctg ctttggtgca acactccagg aagccgcacg
    15601 ccataaatta gaagccaata tgctggacgc gggtggaatc ggcagtatca ccacatgtct
    15661 tagccaggcg gcgttagcgg gtctggcgtc cttcagtcaa caattactgg agcaactcac
    15721 attattaatc gcccaggaaa atcaatttgc cgaaatgggc caggcgctgg aagtgctata
    15781 tgccttatgg cggctggatg aaattagcgg tatgcaaggc gcgcagatat tacaaacgac
    15841 gttatgcgcg gctatcgatc gcacgctgtg gctatgtgaa tctaacggca gaccagatga
    15901 aaaggagttt cacgctcacc tgcatagctg gcaagcgttg tgtcatatcc tgcgcgatct
    15961 acatagcggt gtgcaactcc ccggcatttc tctttctgcg gcggtagcct tactggagcg
    16021 acgcagtcag gcaattcatg ccccggcgct ggatcgcggc gcggctcatg gcgcactgat
    16081 gcgtctggaa catcctaacg ccagtgccga agcggcgctg acgatgctgg cgcagttatc
    16141 cccggcacaa tccggcgaag cgctgcacgg tttgctggcg ctggcccgcc atcaactggc
    16201 ctgtcagccg acatttatcg ccggtttcag cagtcattta aatcaactaa gtgatgccga
    16261 ttttatcaat gccctgcccg atttacgcgc ggcgatggcc tggctaccac cacgagaacg
    16321 cgggacgctg gcgcatcagg tgcttgagca ttatcaactg gcgcaacttc ccgtttcggc
    16381 gctgcaaatg ccgttgcatt gtccaccaca ggccattgca catcatcaac aactcgaaca
    16441 gcaggcactg gcatcgctgc aaaactgggg agttttccat gtctgaactg aacgatcttc
    16501 tgaccacccg tgagctacaa cgctggcgat taattcttgg cgaagcggca gaaacgacgc
    16561 tttgtgggct ggatgacaac gcccggcaga tagaccacgc gctggagtgg ctgtatgggc
    16621 gcgatcctga acggctccag cgtggtgaac gctctggtgg attaggtggc tcaaatctca
    16681 ccacccctga gtggatcaac agtattcaca ctctgtttcc gcaacaggtg attgagcgac
    16741 tggaaagcga tgccgtactg cgctacggca ttgaagatgt ggtgacgaat ctcgacgtgc
    16801 tggaacgtat gcagccttcg gaaagcctgc tacgcgccgt tttgcacacc aaacatctga
    16861 tgaatcccga agtactggct gccgcccgcc agatagtgcg ccaggttgtt gaagaaatta
    16921 tggcgcgact ggcaaaggaa gttcgtcagg ctttttctgg tgtccgcgat cgccgtcgcc
    16981 gctcattaat tccactggcg cgaaactttg atttcaaaag tactctgcgc gccaacctgc
    17041 aacactggca cccgcaacac ggcaagttgt atatcgaatc cccccgcttt aacagccgca
    17101 ttaaacgcca aagcgaacaa tggcaactgg tcttactggt tgatcaaagc ggatcgatgg
    17161 tggactcggt gatccactct gcggtgatgg cggcctgttt gtggcagtta cccggcattc
    17221 gtacccatct ggtggcgttt gacacaagcg tggttgatct cacggcagac gttgccgatc
    17281 cggtagagtt attaatgaaa gtacagttgg gcggcgggac caatatcgcc agtgccgtgg
    17341 agtatggtcg gcaacttatt gaacaaccag caaaaagcgt cattatcctc gtgagcgatt
    17401 tttacgaagg gggttcatca tcattgctga cgcatcaggt gaaaaagtgt gtccagagcg
    17461 gcatcaaagt gctgggactg gcagcgctcg atagcaccgc aacgccttgc tatgaccgcg
    17521 atacggccca ggcgctggtt aatgtcggcg cacaaatagc cgccatgacg ccgggcgagc
    17581 tggcatcatg gcttgcggag aatcttcagt catgaattca ctacgtccgg aattattaga
    17641 actgacaccg caggcactga cggcgttaag caatgccggt tttgttaagc gcagtcttaa
    17701 ggaactggaa aatggcaacg tcccggaaat cagccatgag aacggcgctt taatcgccac
    17761 cttcagtgac ggtgtccgta cccagctggc gaacagccag gcactgaaag aggctcagtg
    17821 cagctgcggg gccagcggca tgtgccgtca tcgcgtgatg ctggtgttaa gttatcaacg
    17881 actttgtgcc accgtccagc ccacggaaaa agaagaagag tgggatccgg caatctggct
    17941 ggaaggactg gctaccctgc ccgatgccac ccgcaaacgc gcgcaggcgc tggtcgctaa
    18001 aggcatcacc attgagttgt tctgtgcgcc gggcgaaatt ccctctgccc gcttaccgat
    18061 gagtgatgtg cgtttttatt cccgtagcag tattcgtttc gcccgttgtg attgtattga
    18121 aggcacactt tgcgaacatg tcgtactggc tgtgcaggcc ttcgtcgagg ccaaagcgca
    18181 gcaagcagaa tttaaccatt taatctggca gatgcgcagc gaacacgtca catcatctga
    18241 cgatccgttt gccagcgaag aaggccaaac gtgtcgtcaa tatgttcagc aattaagcca
    18301 ggcattatgg cttggcggca tcagccagcc gcttattcac tacgaggcag cattcaaccg
    18361 cgcattgcag gcggcagagg cctgcaactg gcgctgggtg agtgaatcac tacgacagct
    18421 acgggcaagc gttgatgcct tccacactcg cgccagccac tatcatgccg gagaatgctt
    18481 acgtcagctt gcggcattaa acagtcgatt aaattgcgca caagagatgg cccggcgcga
    18541 cagtgttggt gaagttcctc ctgtgccgtg gcgcacggtc gttggctctg gcattgccgg
    18601 agaagcaaag cttgatcatc tgcggctggt gtctttaggt atgcgttgct ggcaggatat
    18661 tgagcattat ggtttacgca tctggtttac cgatcccgac accggcagta ttttgcacct
    18721 ttcgcgcagt tggccgcgaa gtgaacagga aaactcaccg gcagctacgc gtcggctgtt
    18781 tagttttcag gctggcgcac tggcgggtgg gcaaattgtt tcacaagcag caaaacgcag
    18841 tgccgatggc gagctgctgt tagctacccg caaccgctta agcagcgttg tgccgctgtc
    18901 gcctgatgcc tggcaaatgt tgagcgcgcc gttacgccag ccgggcattg tggctttgcg
    18961 ggaatattta cgccagcgtc cccccgcctg catacggcct cttaatcagg tcgataactt
    19021 atttattctg ccggtcgctg agtgtatttc gctcggttgg gatagcagcc gccagacgct
    19081 ggatgcgcag gtaatcagcg gcgaaggtga ggataatctc ctgacgttat cccttccggc
    19141 atctgcctgt tctccttttg ccgttgaacg catggcggcg cttttgcaac aaacagacga
    19201 ccccgtgagt ctggtttctg gctttgtcag ttttgttgat gggcaattga cactggaacc
    19261 acgggtgatg atgacaaaaa cccgcgcctg ggcgctggac gcggaaactg cgcctgtggc
    19321 accgctacct tctgccagcg ttttgcctgt gccgtctacc gctcatcagt tgctgatgcg
    19381 ctgccaggcg ttacttattc aactgctcca taacggctgg cgctatcagg aacagagcgc
    19441 tattagtcag gcagagttgc tggcgaatga cctctccgcg gtcggttttt atcggctggc
    19501 acatgtgttg ggacaatttc gtaatacaga aagcgaggca cgggtagaag caatgaataa
    19561 cggtgtttta ctttgcgaac aattattccc catgcttcag caacaaggat gaaatagtgc
    19621 tttttactaa gagttctact ccagttccgg actgctcacg tcacggtatt aggcatattc
    19681 tatatagccc ctggtgagag tcaccagttt cttgattaaa taaaatggag ttttacatga
    19741 aggctttcaa taagctgttt tccctcgttg ttgcatctgt tctggttttc tctcttgctg
    19801 gctgcggtga caaagaagaa tcgaagaaat tcagcgccaa tctgaacgg
//
LOCUS       DAZGJW010000082        19664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZGJW010000082 DAZGJW010000000
VERSION     DAZGJW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.082"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..530
                     /gene="dosC"
                     /locus_tag="ACHKIK_002993"
     CDS             <1..530
                     /gene="dosC"
                     /locus_tag="ACHKIK_002993"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO9084126.1"
                     /translation="LFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSV
                     LIIDVDKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASE
                     NETLRTAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIA
                     KRRGRNRVELWKASL"
     gene            555..2954
                     /gene="dosP"
                     /locus_tag="ACHKIK_002994"
                     /gene_synonym="pdeO"
     CDS             555..2954
                     /gene="dosP"
                     /locus_tag="ACHKIK_002994"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO9084127.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            3212..3793
                     /gene="ddpX"
                     /locus_tag="ACHKIK_002995"
     CDS             3212..3793
                     /gene="ddpX"
                     /locus_tag="ACHKIK_002995"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO9084128.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            3807..5357
                     /locus_tag="ACHKIK_002996"
     CDS             3807..5357
                     /locus_tag="ACHKIK_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO9084129.1"
                     /translation="MKRSISFRPTLLALVLATNFPVSHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            5359..6381
                     /locus_tag="ACHKIK_002997"
     CDS             5359..6381
                     /locus_tag="ACHKIK_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9084130.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            6378..7274
                     /gene="ddpC"
                     /locus_tag="ACHKIK_002998"
     CDS             6378..7274
                     /gene="ddpC"
                     /locus_tag="ACHKIK_002998"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO9084131.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LALAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            7271..8257
                     /locus_tag="ACHKIK_002999"
     CDS             7271..8257
                     /locus_tag="ACHKIK_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9084132.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            8250..9176
                     /locus_tag="ACHKIK_003000"
     CDS             8250..9176
                     /locus_tag="ACHKIK_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO9084133.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTVPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(9232..9663)
                     /gene="osmC"
                     /locus_tag="ACHKIK_003001"
     CDS             complement(9232..9663)
                     /gene="osmC"
                     /locus_tag="ACHKIK_003001"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO9084134.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            10009..10224
                     /gene="bdm"
                     /locus_tag="ACHKIK_003002"
     CDS             10009..10224
                     /gene="bdm"
                     /locus_tag="ACHKIK_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO9084135.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            10326..10463
                     /gene="sra"
                     /locus_tag="ACHKIK_003003"
     CDS             10326..10463
                     /gene="sra"
                     /locus_tag="ACHKIK_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO9084136.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            10620..12317
                     /gene="maeA"
                     /locus_tag="ACHKIK_003004"
     CDS             10620..12317
                     /gene="maeA"
                     /locus_tag="ACHKIK_003004"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO9084137.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            12451..13461
                     /gene="adhP"
                     /locus_tag="ACHKIK_003005"
     CDS             12451..13461
                     /gene="adhP"
                     /locus_tag="ACHKIK_003005"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO9084138.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            13608..13892
                     /locus_tag="ACHKIK_003006"
     CDS             13608..13892
                     /locus_tag="ACHKIK_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO9084139.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(13943..14596)
                     /gene="fdnI"
                     /locus_tag="ACHKIK_003007"
     CDS             complement(13943..14596)
                     /gene="fdnI"
                     /locus_tag="ACHKIK_003007"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO9084140.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(14589..15473)
                     /gene="fdnH"
                     /locus_tag="ACHKIK_003008"
     CDS             complement(14589..15473)
                     /gene="fdnH"
                     /locus_tag="ACHKIK_003008"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO9084141.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(15486..18533)
                     /gene="fdnG"
                     /locus_tag="ACHKIK_003009"
     CDS             complement(15486..18533)
                     /gene="fdnG"
                     /locus_tag="ACHKIK_003009"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(17946..17948),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO9084142.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENDKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            18765..19646
                     /gene="yddG"
                     /locus_tag="ACHKIK_003010"
     CDS             18765..19646
                     /gene="yddG"
                     /locus_tag="ACHKIK_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO9084143.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         4754 a         5065 c         5047 g         4798 t
ORIGIN      
        1 aattgtttga agaagtatcg cgccacgaag tcggtatgga tgtactgacg aaattactta
       61 accgccgttt cctgccgact atcttcaaac gcgaaattgc ccatgccaac cggaccggta
      121 caccactgtc agtgctgatt attgacgttg ataaattcaa agagatcaac gatacgtggg
      181 gccataacac tggcgatgaa attctgcgta aagtctctca ggccttttat gacaacgtcc
      241 gcagtagtga ttatgttttt cgctacgggg gcgatgaatt tatcattgtt ttgactgaag
      301 cttctgaaaa cgaaacgtta cgtaccgcag aacgtattcg cagtcgggtg gagaaaacca
      361 aactgaaagc cgcaaacggc gaggatattg ccctctcact ttccatcggt gccgccatgt
      421 ttaatggtca tcctgactat gagcgcctca ttcaaatagc cgatgaagct ctgtatatcg
      481 ccaaaagacg aggtagaaac cgtgttgaac tctggaaagc cagtctttag atgcgccagg
      541 atgcagaggt aatcatgaag ctaaccgatg cggataatgc cgccgatggc atttttttcc
      601 ccgcccttga gcaaaatatg atgggtgcgg tgttaattaa cgaaaatgat gaagtgatgt
      661 ttttcaaccc cgccgcagag aagctctggg ggtacaaacg tgaagaagtc attggcaata
      721 acattgatat gctgattccg cgggatttgc gtcctgcgca tcctgaatac attcgtcata
      781 accgtgaagg cggtaaagcg cgtgtcgagg ggatgagtcg ggagctgcag ctggagaaaa
      841 aagacggcag taaaatctgg acccgttttg cgctatcgaa agtgagcgcc gaggggaaag
      901 tttattacct ggcgctggta cgggatgcca gcgtagaaat ggcgcaaaaa gaacagactc
      961 gacaattgat tattgccgtt gaccatctcg accgaccggt gattgtcctc gatccggaac
     1021 gccatattgt gcagtgcaat cgcgcattta ccgaaatgtt tggttactgc attagcgaag
     1081 ccagcggtat gcagcccgat acactcctga acattcctga attccctgcc gataaccgca
     1141 ttcgtttaca acagttgcta tggaaaaccg cccgcgatca ggacgaattt ctgctgttga
     1201 cgcgcaccgg tgaaaaaatc tggattaaag cctctatcag cccggtttat gacgtgctcg
     1261 cgcatctgca gaacctggta atgactttct cggatatcac cgaagaacgg caaatccgcc
     1321 agcttgaagg caatattctc gccgccatgt gcagcagccc gccatttcat gaaatggggg
     1381 aaatcatttg tcgtaacatc gaatctgttc tcaacgaatc gcatgtttcg ctgttcgcac
     1441 tgcgcaacgg gatgccgata cactgggcgt catcttcgca cggtgcagaa gttcaaaatg
     1501 cgcaaagctg gtcagcgacc attcgtcagc gtgatggcgc gcctgcgggg atcctgcaaa
     1561 ttaaaacctc gtcaggagca gaaaccagcg cctttatcga acgcgtggca gatatcagtc
     1621 agcatatggc cgcgctggcg ctggaacagg aaaaaagccg ccagcatatt gaacaactca
     1681 tccaatttga tccgatgacc ggtctgccaa atcgcaataa cctgcacaat tacctcgatg
     1741 acctggtcga caaagccgtc tctccggtgg tgtatctcat cggtgttgac catattcagg
     1801 atgtgattga tagccttggc tatgcgtggg ccgatcaggc attgctggaa gtggtcaatc
     1861 gctttcgtga aaaactcaaa ccggatcagt atctctgtcg tatcgaaggt acgcagtttg
     1921 tcctcgtgag cctggaaaac gacgtcagta acattaccca gatcgccgat gagctacgga
     1981 atgtggtcag caagccgata atgattgacg ataaaccatt cccgcttacc ttgagtattg
     2041 gcatcagcta cgacgtgggt aaaaaccgcg attacctact ctccactgct cacaatgcaa
     2101 tggattatat tcgcaagaat ggcggtaacg gctggcagtt cttcagcccg gcgatgaacg
     2161 aaatggtaaa agagcgtttg gttttaggcg cagcgctgaa agaagcgatt agcaataacc
     2221 aactgaaact ggtttaccag ccgcaaatct tcgcagaaac gggtgaactg tacggcatcg
     2281 aagcccttgc tcgctggcac gatccccagc atggtcatgt acccccttca cggtttattc
     2341 ctctcgcaga agagattggt gaaatcgaaa atattgggcg ctgggtcatc gcggaagctt
     2401 gccgtcagtt agcagaatgg cgtagccaga atattcatat cccggcgtta tccgtgaact
     2461 tgtcggcgct gcactttcgc agtaatcagc tgcctaatca ggtgtctgat gcaatgcaag
     2521 cctggggtat tgacggccac cagctgacgg tagaaatcac ggaaagcatg atgatggaac
     2581 acgataccga aatctttaag cgcattcaga tcctgcgtga tatgggcgtg ggcttatcgg
     2641 tagatgattt tggtacgggc ttttccggat tatcccgctt agtcagtctt ccggtaacgg
     2701 aaatcaaaat tgacaaaagt tttgtcgatc gttgtctgac cgaaaaacgc atccttgcct
     2761 tacttgaagc cattaccagc attgggcaaa gcctcaattt aaccgtcgtg gcggaaggcg
     2821 tcgaaaccaa agagcaattt gagatgctac gcaagatcca ctgtcgcgtt attcagggat
     2881 atttcttttc ccgcccccta cccgccgaag aaattccagg ctggatgagc agcgtgttac
     2941 cgctgaaaat ctgacaaatt cctctcgccc gcactcgcgg gttttcattt aacgtgacac
     3001 tgtcacttaa atactgtgat ttcagctacg attccgggag attccttcct taacttcccc
     3061 tcttgcacca aaactgcact acgctgcaca aatgttgatc aaaagttaaa aaatataact
     3121 ttaagccagc attctggcgc ttatcccggc atggcatgag atctgcataa gcggaaagcg
     3181 cagcaatttt tgtcttatac aggaaccgtt tatgtcggat accaccgaac tggttgattt
     3241 agccgtaatc ttccctgatc tggagatcga attgaaatac gcctgcgctg ataacatcac
     3301 aggtaaagct atttatcagc aagcgcgttg tctgctacac aaggatgcga ttaccgcgct
     3361 ggcgaaaagt atcagtatcg cccagctgtc aggcttacaa ctggtgattt acgatgcgta
     3421 tcgcccacaa caagcacagg cgatgttgtg gcaagcctgc ccagacccgc aatatgttgt
     3481 tgatgtgacg gtcggttcta atcacagccg tggcacggcg atcgacctga cgcttcgtga
     3541 tgagcacggg aacatcctcg atatgggcgc agggttcgat gaaatgcacg agcgctccca
     3601 tgcctatcac ccttccgtcc cgcccgccgc tcagcgcaat cggctgttgc tgaatgcgat
     3661 aatgactggt ggcggttttg tcggtatctc cagcgaatgg tggcacttcg aattacccca
     3721 ggcagcgagt taccctctgc tcgctgatca attctcttgt tttatatccc ctggcacaca
     3781 gcacgtcagt taatcaggaa actgtcatga agagatcgat atcgtttcgt cccacattgc
     3841 tcgcgctcgt ccttgccaca aatttcccgg tttcgcacgc cgccgtaccg aaagatatgc
     3901 tggtgattgg taaggccgcc gatccacaaa ccctcgaccc ggcggtaaca atagataata
     3961 acgactggac ggtgacctac ccgtcttatc agcggctggt tcagtacaaa acggacggtg
     4021 ataaaggctc aaccgacgtt gaaggcgatc tggcaagtag ctggaaagcg tctgacgatc
     4081 aaaaagagtg gacgttcacc ctgaaagata atgctaaatt tgccgatggc acacctgtca
     4141 ctgccgaagc agtaaaactt tcttttgagc ggctactaaa aatcggccag gggccagcag
     4201 aagcatttcc caaagattta aagattgatg ctcccgacga acatacggtg aagtttaccc
     4261 ttagccaacc attcgcaccg ttcctctaca cgctggcgaa tgacggtgca tccattatca
     4321 atccggcggt cttaaaggaa catgcagcgg atgatgctcg cggcttcctc gcgcaaaata
     4381 ccgccggttc cggaccattt atgctgaaaa gctggcaaaa aggtcagcaa ttagttctgg
     4441 tgccaaatcc gcattacccc ggcaataaac cgaactttaa gcgagtatcg gtaaaaatta
     4501 tcggtgaaag tgcctcccgt cgcctgcagc tctcccgtgg cgacattgac attgccgatg
     4561 cgctgccggt ggatcaactc aacgccctga agcaggaaaa taaagtcaat gtggcagagt
     4621 atccgtcact gcgcgttacc tatctgtatc tgaataacag caaagcgcct cttaatcagg
     4681 cggatctacg tcgggccatt tcctggtcta ccgattatca gggcatggtt aacggcattc
     4741 tgagtggtaa cggaaaacag atgcgcggcc cgattccgga aggcatgtgg ggctacgatg
     4801 cgacggcaat gcaatacaac catgacgaaa cgaaagccaa agccgaatgg gataaagtga
     4861 cgagcaaacc caccagcctg acgtttctct actccgataa cgatccgaac tgggagccta
     4921 ttgctttggc gacacaatcc agtctcaaca agctgggtat caatgtgaag ctggaaaagc
     4981 tggcgaacgc caccatgcgc gacagagtgg gtaaaggtga ttacgacatt gcgattggca
     5041 actggagtcc ggattttgcc gacccgtata tgtttatgaa ttactggttt gagtcagaca
     5101 aaaaaggtct gccgggtaac cgctcgttct atgaaaacag tgaggtcgat aagttactgc
     5161 gcaatgcgct tgcgaccacc gaccagacgc agcgtacccg ggactaccag caggcacaga
     5221 aaatcgtcat tgatgacgct gcttatgtgt acctgttcca gaaaaactac caactggcga
     5281 tgaacaaaga ggtgaaaggc tttgtgttca atcccatgct ggaacaggtc ttcaatatca
     5341 ataccatgag taaataacgt gaccttctgg agtattttac gccaacgctg ctgggggctg
     5401 gtgctcgtgg tggcgggcgt ctgcgtgatt acgtttatta tctcgcatct gatccctggc
     5461 gatccggcgc ggttactggc gggtgaccgc gccagcgatg ctatcgtgga aaatattcgc
     5521 cagcaactgg gactggacca gccactgtat gtacagtttt accgctacgt cagcgacctg
     5581 tttcatggtg acctgggaac atccattcgt accgggcgtc cggtgctgga agagttgcgt
     5641 atatttttcc cggcgacgct ggagctggct tttggtgccc tgctgctggc actcctgatt
     5701 ggcatcccgc tgggcatact ctctgcagtc tggcgaaatc gctggctgga tcatctggtg
     5761 cgaataatgg ccattaccgg aatctccaca cctgcgttct ggcttgggct gggcgtcatt
     5821 gtgctgtttt atggtcatct gcaaattctt cccggcggcg gaaggcttga tgactggctg
     5881 gatccaccaa cgcacgttac cggcttttat ctgctcgatg cgctgcttga aggcaacggt
     5941 gaagtcttct tcaatgcgtt gcaacatctc atcttaccgg cattaacgct ggcgttcgtt
     6001 cacctgggaa ttgtcgctcg ccagatccgc tcagcgatgc tggaacaatt gagtgaagac
     6061 tacattcgta ccgcccgggc cagcggcttg cccggctggt atatcgtttt atgttatgcg
     6121 ctacccaatg cgttgatccc atcgattacc gtattgggtt tggcgctggg cgatttgttg
     6181 tatggcgcag tgctcaccga aaccgttttt gcctggcccg gaatgggtgc atgggtagta
     6241 acatcaatac aggcgctcga cttcccggca gtgatgggct ttgccgtcgt ggtttcattt
     6301 gcttatgtgc tggtcaacct ggtggtggat ttgctctatt tgtggattga tccgcgtatc
     6361 ggacgtggag gtggtgaatg atgctaagcg aggaaacgtc cgccgtacgc ccacaaaaac
     6421 aaacgcgatt taacggtgca aaactggtat ggatgctgaa aggcagtccg ctcaccgtga
     6481 ccggcgcagt catcattgta ttaatgctat tgatgatgat tttttcaccg tggctggcga
     6541 cgcatgatcc caacgccatt gatttaaccg cccgcctttt gccgccttct gcggcgcact
     6601 ggtttggcac cgatgaagtg ggacgcgatc tgttcagccg cgtactggtc ggcagtcagc
     6661 aatcaattct cgccggatta gtcgtggttg ccattgcggg tatgattggt tcgctactcg
     6721 gatgtctatc cggtgtgctt ggcggacgcg cagacgccat cattatgcgc atcatggaca
     6781 ttatgctgtc gattccttcg ttggtactga caatggcact ggctgccgct ctcgggccga
     6841 gtttatttaa cgccatgctg gcgcttgcta ttgtgcgaat tcccttttat gtgcgcctgg
     6901 cgcgggggca agcattagtt gtacgccagt atacctatgt tcaggcggcg aaaacctttg
     6961 gtgcgtcgcg ttggcatctg atcagctggc atattttacg taactccctg ccgccgctga
     7021 tcgtgcaggc atcgctggat atcggtagcg cgattttaat ggccgccacg ttgggattta
     7081 ttggcctggg tgctcaacaa ccgagtgctg aatggggcgc gatggtagcg aatggtcgca
     7141 actatgtact cgatcagtgg tggtattgcg catttccggg ggcagcgatt ttgcttaccg
     7201 ccgttgggtt taatctcttt ggtgatggta ttcgcgatct gcttgacccg aaagcaggag
     7261 gaaagcagtc atgacccaac ccgttctgga cattcaacag ctgcatttga gtttccccgg
     7321 ttttaacggt gatgttcacg cgctcaacaa tgtgtccttg cagattaacc gcggcgaaat
     7381 tgtcggtctg gtgggagaat ccggctcagg taaatcagtc accgcaatgc tgattatgcg
     7441 tctgctacag acgggcagtt attgcgtaca tcgggggcag atttcactgc tgggagacga
     7501 tgttcttaac gctcgggaaa aacagcttcg tcagtggcgc ggcgcacgag tggcgatgat
     7561 ctttcaggaa ccgatgaccg ccctcaatcc gacacttcga ataggtcttc agatgatgga
     7621 cgtgatccgc catcatcaac caataagtcg tcgggaagcc agagctaaag cgattgccct
     7681 gctggaagag atgcaaatcc cggatgccgt ggaagttatg tcgcgctatc cgtttgagct
     7741 ttcaggtggt atgcgccagc gggtaatgat tgcgctggca ttctcctgcg agccgcaatt
     7801 gattattgcc gacgaaccga ctacggcgct ggacgtcacg gtgcagttgc aggtactgcg
     7861 tttgcttaaa cataaagccc gcgccagtgg aactgcggtt ctgttcatca gccatgatat
     7921 ggccgtggtg tcgcaactgt gcgatagcgt ttacgtgatg tatgccggaa gcgtaattga
     7981 aagcggcgtg acggcagatg ttatccatca tccccggcat ccgtatacca ttggtttgct
     8041 ccaatgcgca ccagaacatg gagtaccacg ccagctatta cccgccattc cagggacggt
     8101 accaaacctc acccatttgc ctgaaggctg cgcttttcgc gatcgttgct atgcggcagg
     8161 tgcacagtgt gaaaacgtcc cggcgctgac agcgtgtggt gacaacaacc agcgctgcgc
     8221 ctgttggtat ccgcagcagg aggtcataag tgtctgacac gttattaacg ttacgcgacg
     8281 tccatatcaa tttcccggcc cgtaaaaact ggcttggtaa aactacggaa catgttcatg
     8341 ccattaatgg tattgattta cagatccgcc gtggtgaaac cttagggatc gtcggcgagt
     8401 caggctgcgg caaaagcacc ctcgcacagc ttttaatggg catgctgcaa ccgagccacg
     8461 ggcagtacat ccgttctggc tcacaacgca ttatgcagat ggtgtttcag gacccgctct
     8521 cttcgctcaa tccgcgctta ccggtgtggc gcatcatcac agaaccgctc tggatagcta
     8581 agcgtagtag tgaacaacag cggcgagcgt tggcagagga gctggctgtg caggtgggta
     8641 ttcgtccgga gtatctcgac cgcctgcctc atgcgttctc cggcgggcag cggcaacgca
     8701 tcgccattgc cagagcactc tcttcgcagc ctgacgtgat tgtgcttgat gagccaacct
     8761 cggcgctgga tatctccgtg caggcgcaga tcctcaattt actggtaacg ctacaggaaa
     8821 atcacgggct gacttatgtg ctgatttcac acaatgtctc ggtgatacgt catatgagcg
     8881 atcgggtggc ggtgatgtat ctcgggcaga ttgtggaact aggggaagcg caacaggtgt
     8941 tgactgttcc ggcacatcca tatacccgat tattgctgga ttccctcccc gccattgata
     9001 aaccgctgga ggaagaatgg gcattacgta aaacggatct gccgggaaac cgcacgttgc
     9061 cgcaaggctg ttttttctac gaacgttgcc ctctggcaac ccacggatgt gaagtccggc
     9121 aatcattaac aataagggag gacggacgtg agatccggtg ctggcgggcg ctgtagagat
     9181 gctaacaacg catcaggcat tcacgcgcct gacgcgtcat ccggcaatgc tttacgattt
     9241 caactggtaa tcaagcgtaa tttccgcttt cagtacctga gaaaccgggc atcctgcttt
     9301 tgctttctgg atgatgccgt caaaggtaga ggcatcaata cccggcaccg caacttcact
     9361 cttcagtgcg attttcgtaa tcgcaaaacc ggcatccact ttatccagcg acacatcggc
     9421 ggtggtatca atcgatgttg gcgtaaatcc cgcttccccc agcattaatg aaagcgccat
     9481 tgagaaacat gcggcatgcg ctgcgccaat cagttcttca gggttagttc ctttttcgcc
     9541 ttcaaaacgc gtgttaaatc catacggctg ttggttcagc acaccactct cggtggatac
     9601 tgttcccttc ccgcgtttga tatcgccttc ccagtgtgcc tgacctttct tatggattgt
     9661 cattgttgct ctcctgtggg cttaaaaagg aaattataga ctacgtgaga aacagcacga
     9721 ggataagcag gatattccga ataaaaatcc ggcagaaata ttacttctgc acttaaatta
     9781 aatttccaca atagataacc gggaatatat aacccaagag atattgcatt aatattaatc
     9841 aactcaccac aataataata cctatcaata tcatgaggtt aaaatataaa aatcatcaac
     9901 caggactaat cttaacaacg aagcggcaaa tatttgccat gctgaatgtg ctttataaat
     9961 ctgcgatccg tagcagacac cataaataca cagacacgga gaatcactat gtttacttat
    10021 tatcaggcag aaaattcaac agcagaaccc gctctggtta atgccattga gcaaggcctt
    10081 cgggcagagc acggtgttgt cactgaagat gacattctca tggagctgac caaatgggtg
    10141 gaagcttctg ataacgacat cctcagtgat atctaccagc aaacgatcaa ttatgtggtc
    10201 agtggccagc accctacgct ttaaggtgct atgcttgatc ggcaacctaa tttaggggtt
    10261 tagcacgtgt ttcttcgcta cggcgatgtt gtccttaaaa ctagcaacag gattgaggag
    10321 ttaaaatgaa atcgaaccgt caggcacgtc atattcttgg actggaccat aaaatttcta
    10381 accagcgcaa aatagttacc gaaggtgaca aatccagcgt ggtaaataat ccaaccggca
    10441 gaaaacgccc cgctgaaaag taattcataa ccatcagtcc tcaatgacga ttaaacacca
    10501 ttgcctgcgc aatggtgttt ttgtttttat ctgctctata cttggggccg acgccctggc
    10561 ggtaaagcaa agacgataaa agcgtgccag ggatggatat tcaaaaaaga gtgagtgaca
    10621 tggaaccaaa aacaaaaaaa cagcgttcgc tttatatccc ttacgctggc cctgtactgc
    10681 tggaatttcc gttgttgaat aaaggcagtg ccttcagcat ggaagaacgc cgtaacttca
    10741 acctgctggg gttactgccg gaagtggtcg aaaccatcga agaacaagcg gaacgagcat
    10801 ggatccagta tcagggattc aaaaccgaaa tcgacaaaca catctacctg cgtaacatcc
    10861 aggacaccaa cgaaaccctc ttctaccgtc tggtaaacaa tcatcttgat gagatgatgc
    10921 ctgttattta taccccaacc gtcggcgcag cctgtgagcg tttttctgag atctaccgcc
    10981 gttcacgcgg cgtgtttatc tcttaccaga accggcacaa tatggacgat attctgcaaa
    11041 acgtgccgaa ccataatatt aaagtgattg tggtgactga cggtgaacgt attctggggc
    11101 ttggtgacca gggcatcggc gggatgggca ttccgatcgg taaactgtcg ctctataccg
    11161 cctgtggcgg catcagcccg gcgtataccc ttccggtggt gctggatgtc ggaacgaaca
    11221 accaacagct gcttaacgat ccgctgtata tgggctggcg taatccacgt atcactgacg
    11281 atgaatacta tgaattcgtt gatgaattta tccaggctgt gaaacaacgc tggccggacg
    11341 tgctgttgca gtttgaagac tttgctcaaa aaaatgcgat gccgttactt aaccgctatc
    11401 gcaatgaaat ttgttctttt aacgatgaca ttcagggcac tgcggcagta acagtcggca
    11461 cactgatcgc agccagccgc gcggcaggtg gtcagttaag cgagaaaaaa atcgtcttcc
    11521 ttggcgcagg ttcagcggga tgcggcattg ccgaaatgat catcgcccag acccagcgcg
    11581 aaggattaag cgaggaagca gcgcggcaga aagtctttat ggtcgatcgc tttggcctgc
    11641 tgacggacaa gatgccgaac ctgctacctt tccagaccaa actggtgcag aagcgcgaaa
    11701 acctcagtga ctgggatacc gacagcgatg tgttgtcact gctggatgtg gtgcgcaatg
    11761 taaaaccaga tattctgatt ggcgtctcag gacagaccgg gctgtttacg gaagagatca
    11821 ttcgtgagat gcataaacac tgtccgcgtc cgatcgtgat gccgctgtct aacccgacgt
    11881 cacgcgtgga agccacaccg caggacatta tcgcctggac cgaaggtaac gcgctggtcg
    11941 ccactggcag cccgtttaat ccagtggtat ggaaagataa aatctaccct atcgcccagt
    12001 gtaacaacgc ctttattttc ccaggcatcg ggctgggtgt tattgcttcc ggcgcgtcac
    12061 gtatcaccga tgagatgctg atgtcagcaa gtgaaacgct tgctcagtat tcgccgctgg
    12121 tgctgaacgg cgaaggtctg gtactaccgg aactgaaaga tattcagaaa gtctcccgcg
    12181 caattgcgtt tgcggttggc aaaatggcgc agcagcaagg cgtggcggtg aaaacgtctg
    12241 ccgaagctct gcaacaagcc attgacgata atttctggca agccgaatac cgcgactacc
    12301 gccgtacctc catctaagcc tgcgcccggt agtgaaggct accgggctat ttccctctcc
    12361 ctttttcaga tctcatccat actgggtagt ggcgaataaa tctcatttgc ctcacctgct
    12421 atgcagaaca tcatccgaaa aggaggaact atgaaggctg cagttgttac gaaggatcat
    12481 catgttgacg ttacggataa aacactgcgc tcactgaaac atggcgaagc cctgctgaaa
    12541 atggagtgtt gtggtgtatg tcataccgat cttcatgtta agaatggcga ttttggtgac
    12601 aaaactgggg taattctggg ccatgaaggc atcggtgtgg tggcagaagt gggtccaggt
    12661 gtcacctcat taaaaccagg cgatcgtgcc agcgtggcgt ggttctacga aggatgcggt
    12721 cattgcgaat actgtaacag tggtaacgaa acgctctgcc gttcagttaa aaatgccgga
    12781 tacagcgttg atggcgggat ggcggaagag tgcatcgtgg tcgccgatta cgcggtaaaa
    12841 gtgccagatg gtctggactc ggcggcggcc agcagcatta cctgtgcggg ggtcaccacc
    12901 tacaaagccg ttaaactgtc aaaaattcgt cccggtcagt ggattgctat ctacggtctt
    12961 ggcggtctgg gtaacctcgc cctgcaatac gcgaagaatg tctttaacgc caaagtgatc
    13021 gccattgatg tcaatgatga gcagttaaaa ctggcaaccg aaatgggtgc agatttagcg
    13081 attaactcac gcaccgaaga cgccgccaaa attgtgcagg agaaaaccgg tggcgctcac
    13141 gctgcggtgg taacagcagt tgctaaagct gcgtttaact cggcagttga tgctgtccgt
    13201 gcaggcggtc gtgttgtggc tgtcggtctg ccgccggagt ctatgagcct ggatatccca
    13261 cgccttgtgc tggatggcat tgaagtggtc ggttcgctgg tcggcacgcg ccaggatcta
    13321 accgaagcct tccagtttgc cgccgaaggc aaagtggtgc cgaaagtcgc cctgcgtccg
    13381 ttagcggaca tcaacaccat ctttaccgag atggaagaag gcaaaatccg tggccgtatg
    13441 gtgattgatt tccgccgtta agaggccttt gctgcgagtg ccagtcctgg tgtcgcagca
    13501 tcgcactctc tccgacatta aatggctaaa tggattattg ttccttccta cgcaacaata
    13561 atgatagtgt tcctgatgaa ggaacactac tgagagtaaa gacactgatg aaaatggcca
    13621 atcatccccg cccgggggac attattcagg aatcactgga cgaacttaat gtcagcctgc
    13681 gcgagtttgc cagagcaatg gaaattgctc cctcaacggc aagccgattg ctgaccggaa
    13741 aagcagcttt gacaccagaa atggcgatta aactttccgt ggtgatcggc agttcgccgc
    13801 aaatgtggct gaatctgcaa aacgcctgga gtctggcaga ggcagaaaaa acggtggatg
    13861 tgtcgcgact acgccgtctg gttacgcaat aaaaaaatgg cgcttcttaa agcgccattt
    13921 tcagcttaaa gttcaaaaga gattatatcc cttcttcact ctcttttttc gcttctgcct
    13981 tctcgatttc acgataccag cgcggatggt gtttcttcgc ccagcgacgg ctcaccttcc
    14041 cttcgatcat ccctttaatc gaccctttca cccagaatgc catatacata tggatcagga
    14101 tggcgtggat caggatgata cccgcagccg cgtggatcag caggctgtag cgaacaacct
    14161 gcatcgggaa gtactgcgca aagtacggac gccagataat caccccggtc accagcagca
    14221 cgaaaatcat gctcatgatc gaccagaaca tcatcttttg cccggcgttg tacttaccga
    14281 catccgccac tttatgctca ttgcctttca atacttcgac aatgttcaac agccacggaa
    14341 tatctttctt atccgggatg ttgtgatgca caaaacgcac aaacataaac atcagtgcga
    14401 cgaaaatcgc aatgccgaaa aacgggtgca aaatgcgtcc catctgcggc gtaccgaagg
    14461 tttgcgtcag ccattgcagc gtcgggaaga agaacgaaat cccggacagc gccaccagga
    14521 agaagcaaat caccacggtc cagtgacagg cgcgatcaat aaatttggtg cgcacaatca
    14581 ttttcgactt actcatgatg atcctcctcg tcatcgtcca cttccttatt cgggccaata
    14641 ccgatgtagt ggaaaatcaa cccggcaaaa gtggcaataa agccagccgc tgccagcggt
    14701 ttcaacgcgc ctttccacag gcttaccgag gtgtcgatct tcggatcttt cggcagaccg
    14761 tgatacagct ccggctgatc ggcgtgatgc agcacgtaca taacgtgcgt accaccgacc
    14821 ccttccgggt tgtagacgcc agcatgttcg taaccacgcg ctttcagttt cgccacgcgc
    14881 tgttccgcca gctccagcat ctccttcttg gtgccgaagt ggatagcccc ggtcggacag
    14941 gttttcacac aagccggttc ctggccgacg ctgacgcgat cgacgcagag cgtgcattta
    15001 tatacccggt tatcctcttt gttgaggcgc ggaatattaa acggacaccc ggcaatgcag
    15061 taaccacagc cgatgcagtt ttccgactgg aaatcgacaa tcccgttagc gtactgaatg
    15121 attgcaccag cagacgggca cgccttcagg cagccgggat cttcacagtg catacagccg
    15181 tctttacgga tcagccactc cagcttgccg ttctgttcgg tttcgctaaa gcgcatcacc
    15241 gtccaggact tggcgctcag atcggcgggg ttatcgtaaa ccccgacgca gtgccccact
    15301 tcatcacgga tgtcgttcca ctccgaacac gccacctgac aggctttaca gccgatacag
    15361 gtggaaacgt cgataagttt tgcgacttct gctttgtaat cacgcacctg agaaggcggc
    15421 gtgatggagt tagttgcgga ccttttgata atgtcctgcg tttccatagc catctgttcg
    15481 cccccttacg ccttctcgat gttgactaag aacgctttat attccggcgt ttgcgagttt
    15541 gcatcaccga cattcggcgt cagagtgtta gcgatataac ctttacgcgc gacaccctca
    15601 aagccccagt ggattggaat acccaccgtt tcaacctgct gaccatttac attcagtggt
    15661 ttcagacgac gcgttaccac agccaccgcg cggataaagc cacgcttgct tgagacagtg
    15721 acacgatcgc cattattaat gcctttcgcc gccgccagcg tttcgctgat ttccacaaac
    15781 tgttccggct gagcaattgc gttgagcaat gcgtgcttgg tccaggtgtg gaagtgctcg
    15841 gtcagacgat aggtcgtacc cacatacggg aactgctctt ttttacccat ccgcagtgcg
    15901 tcttgttcat acagacgaac aaccgggtta gacaccacgt tcggatgcag cgggttagtg
    15961 cccagcggcg tttcaatcgg ctcgtagtgt tccgggaacg gaccttccgc cattttgttg
    16021 atggcaaaca ggcgtcccat cccttccggc tgcatgataa acggcccggt tggcgtaccc
    16081 ggtgcggcat tgccgaagtc aggaatatcg ttacccgtcc acttgctgcc gttccactgg
    16141 atcagcatcc gtttcggatc ccacggttta ccgttgatat ccgccgaagc acggttgtac
    16201 agcacgcgac ggttgagcgg ccacgcccag gcccatccca gcgtattccc cagaccggac
    16261 gggtcggagt tatcgcggtt agccatctgg ttgccctgct ctgtccagct accggtgtag
    16321 atccagcaag aagatgcggt tgtaccgtca tcacgcagat gcgcaaagct actcagcaac
    16381 tgacctttct tcgcaatcag cacgccatta gcatcataga gatcttccag cgcatagccg
    16441 ttgttctctt tagctacttc gtcagattgc ggttcgtgcg gctgcttgta gttccagctc
    16501 atcttcatca gcggttctac gcctttacca ccttcggcct ggtacagctc gcgcaggtga
    16561 tggtagatac ccgccagaat ttcaccgtcg ttacgcgctt cgcccggcgc atcctgacct
    16621 ttccagtgcc actgcagcca gcgaccggag ttagcaatag aaccatcttc ttcagcaaag
    16681 caggtcgaag gcagacggaa tacttcagtc tgaatagacg ccggatcgac atcgttcgac
    16741 tcgccgtggt tctgccagaa ggttgaggtt tcagtcacca gcggatcgat aaccaccatg
    16801 tacttcagct tgcttaggca gctcaccact ttgtttttgt ccgggaagga cgcaaccggg
    16861 ttaaagccct ggcagaaata accggtgact ttgccttcat ccatcatgtt gaaatacttg
    16921 atgacgtcgt aggtctggtc ccacttcggc agccagtcat agccccagtt gttctctttc
    16981 tgcgcggcat cgccatagaa agatttcatc aggctaacga agaacttcgg atagttgctc
    17041 cagtagttca cctgaccagc cagcgtcgct ttcggcgtgt tcgcttccag atacgactgc
    17101 aaatcaacct gtttttctga cggcagcgtc agataacctg gcaggctggt agagagcaga
    17161 cctaagtcag tcagaccctg aatgttggag tgaccacgca atgcattcac gccgccaccg
    17221 gccataccca tgttaccgag cagcaactgg atcatcgcca tagtacggat gttctgcgca
    17281 cccacggtgt gctgcgtcca gcccagcgcg tacaggaagg tggttgtgcg atccggtgcg
    17341 ctggtggagg ccagcacttc acacactttc aggaagtcgg cttttggcgt accacagatg
    17401 ttttcaacaa cgtccggcgt gtagcgggaa acgtgcgctt tcagcaggtt ccacacacag
    17461 cgcggatgag tcagtgtttc atcgcgtttc gcatagccgt tttcatcgaa ctgatagttc
    17521 caggacgatt tatcgtattg ccgtttttca gcgtcgtagc cgctgaacag accgtcttcg
    17581 aaagcaaaat catcacgcac cagcaggctg gcgttggtgt aatgcttaac gtattcggcg
    17641 ttgattttgt cgttttcgat caggtagcgc aaaacgccag acaggaacgt aatgtccgta
    17701 ccggaacgaa taggtgcgta aatatccgcc acagaagcgg tacgcgtaaa acgaggatcg
    17761 acaacgatca aggttgcatc gttgttgttt ttcgcttcca tcgcccagcg gaaaccgacg
    17821 ggatgcgctt cagcagcgtt accgcccatt accattacga cgttagcgtt tttgatatcc
    17881 acccagtggt tggtcatcgc accgcgacca aatgttggag caagacttgc taccgttggt
    17941 ccgtgtcaga cgcgcgcctg gttgtctacc gccagcatcc cgagggagcg ggcaaatttc
    18001 tgtgtcagca tcccggtttc gttgctggca ccggaggcac acagcatacc ggtagaaagc
    18061 caacggttta ccgttacgcc ctgctcgttc ttttcaataa agttagcgtc acggtcagct
    18121 ttcatcagct ttgcaatacg ggagaatgct tcttcccagc taatgcgctg ccatttgtca
    18181 gaacctggcg cacgatattc cgggtagcgc agacggtttt cgctgttgac gtaatccagc
    18241 aaaccggccc cttttgggca cagcgcacca cggcttaccg gatgatccgg gtcaccttca
    18301 atgtgataaa tcgcttctct ggcgtttttc gcgccatcac ccaggctata catcaatagc
    18361 ccgcaaccta cggaacagta tgtgcaggtg ttacggatct ctttagcgcg taataatttg
    18421 tagtttcgcg cctgagccag tgcttgcttc ggggcaaagc ccaatgccgc taccgttgtt
    18481 ccagccatac cgcccgcgca gattttaaaa aattgtctgc gactgacgtc cattgctttc
    18541 ctcttttttc aggggtatta ctgcgcgagg aaactaacag taaaattctg aatctttttg
    18601 cgcgaaatca aaaagaacac gtgtttgcca caaaataatc cgcctgcgca aattgaccta
    18661 cctcaatagc ggtagaaaaa cgcaccactg cctgacaggc cagttaaaaa aatgctataa
    18721 aattcagctt aatttttaac ggcaagagag acaaaacagc gagcatgaca cgacaaaaag
    18781 caacgctcat agggctaata gcgatcgtcc tgtggagcac gatggtagga ttgattcgcg
    18841 gtgtcagcga ggggctcggc ccggtcggcg gcgcagctgc tatctattca ttaagcgggc
    18901 tgctgttaat cttcacggtt ggatttccgc gtattcggca gattccgaaa ggctatttac
    18961 tcgccgggag tctgttattc gtcagctatg aaatctgtct ggcgctttcc ttagggtatg
    19021 cggcgacccg tcatcaggcg attgaagtgg gtatggtgaa ctatctgtgg cccagcctga
    19081 caattctctt tgccattctg tttaatggtc agaaaaccaa ctggctgatt gtaccaggat
    19141 tattattagc cctggtcggc gtctgctggg tgttaggcgg tgagaatggg ttacattatg
    19201 atgaaatcat caataatatc accaccagcc cattgagtta tttcctggcg tttattggtg
    19261 cgtttatctg ggcagcctat tgcacagtaa cgaataaata cgcacgcgga tttaacggaa
    19321 ttaccgtttt tgtcctgcta acgggagcaa gtctgtgggt ttactatttt ctcacgccac
    19381 aaccagaaat ggtatttagc acgcccgtca tgattaaact catctctgcg gcatttacct
    19441 taggatttgc ttatgctgca tggaatgtcg gtatactgca tggcaatgtc accataatgg
    19501 cggtaggctc gtattttacg cccgtacttt cctcagcgct tgcagccgtg ctgctcagcg
    19561 ccccgctgtc gttctcattc tggcaaggcg cgctaatggt ctgcggcggt tccctgctct
    19621 gctggctggc gacacgtcgt ggttaaaata attgccgggt ctac
//
LOCUS       DAZGJW010000083        19450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZGJW010000083 DAZGJW010000000
VERSION     DAZGJW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.083"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            129..2339
                     /gene="katP"
                     /locus_tag="ACHKIK_003011"
     CDS             129..2339
                     /gene="katP"
                     /locus_tag="ACHKIK_003011"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HFO9084144.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVAEVYASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            2383..2772
                     /locus_tag="ACHKIK_003012"
     CDS             2383..2772
                     /locus_tag="ACHKIK_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO9084145.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            2886..3653
                     /locus_tag="ACHKIK_003013"
                     /pseudo
     CDS             2886..3653
                     /locus_tag="ACHKIK_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    3166..3276
                     /locus_tag="ACHKIK_003013"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(3733..4128)
                     /locus_tag="ACHKIK_003014"
     CDS             complement(3733..4128)
                     /locus_tag="ACHKIK_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO9084146.1"
                     /translation="MESSDPKKRKKVVAYLHPTLYPQDNLTQQTIDSLPIQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIG
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            complement(4128..5087)
                     /locus_tag="ACHKIK_003015"
     CDS             complement(4128..5087)
                     /locus_tag="ACHKIK_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO9084147.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNITHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5360..6262
                     /locus_tag="ACHKIK_003016"
     CDS             5360..6262
                     /locus_tag="ACHKIK_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO9084148.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTYLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWYQPESDVIAELSRRFSCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSHP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            6259..6570
                     /locus_tag="ACHKIK_003017"
     CDS             6259..6570
                     /locus_tag="ACHKIK_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084149.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            6646..7329
                     /locus_tag="ACHKIK_003018"
     CDS             6646..7329
                     /locus_tag="ACHKIK_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO9084150.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWHRIDRFMAVWIRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNSIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNRFEPEAA"
     gene            7329..7547
                     /locus_tag="ACHKIK_003019"
     CDS             7329..7547
                     /locus_tag="ACHKIK_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084151.1"
                     /translation="MNYSGFEDMREQVAELANTMCDLRTTMNEMEQRYSDADTLPERL
                     VRQTLFRASHLFMKAYNEILELDYCFKD"
     gene            7507..7993
                     /locus_tag="ACHKIK_003020"
                     /pseudo
     CDS             7507..7993
                     /locus_tag="ACHKIK_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            8038..8277
                     /locus_tag="ACHKIK_003021"
                     /pseudo
     CDS             8038..8277
                     /locus_tag="ACHKIK_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8272..8520
                     /gene="psiB"
                     /locus_tag="ACHKIK_003022"
                     /pseudo
     CDS             8272..8520
                     /gene="psiB"
                     /locus_tag="ACHKIK_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
     gene            8517..9236
                     /locus_tag="ACHKIK_003023"
     CDS             8517..9236
                     /locus_tag="ACHKIK_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO9084152.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            9233..9830
                     /locus_tag="ACHKIK_003024"
                     /pseudo
     CDS             9233..9830
                     /locus_tag="ACHKIK_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10292..10792
                     /locus_tag="ACHKIK_003025"
     CDS             10292..10792
                     /locus_tag="ACHKIK_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO9084153.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPVNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(10794..11414)
                     /locus_tag="ACHKIK_003026"
     CDS             complement(10794..11414)
                     /locus_tag="ACHKIK_003026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084154.1"
                     /translation="MDLSDGAATATARGSCPGADAKVFDACGKKFCGWYIFPWTAAPC
                     CGRFSPRKVHSGKQAAGVAAEGLTAPSGAVALHTVRCAGSAVSGATGAARPERSLQAW
                     FFRHRTVAAARNRASGFGGDWRVRQQLPVGVGPTTGCRQAAGSRRVSPPPGIRTTETT
                     KQKNASHHDGQEGQRRRRGPPVFQAGVSLPGFSGGPAGHTPAGAGE"
     gene            11520..11954
                     /locus_tag="ACHKIK_003027"
     CDS             11520..11954
                     /locus_tag="ACHKIK_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFO9084155.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQLA
                     EALVEHGDIRCLGPRCDKSAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            12047..12313
                     /locus_tag="ACHKIK_003028"
     CDS             12047..12313
                     /locus_tag="ACHKIK_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084156.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENIPLHRHTRVRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERACH"
     gene            12378..13268
                     /locus_tag="ACHKIK_003029"
     CDS             12378..13268
                     /locus_tag="ACHKIK_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO9084157.1"
                     /translation="MSKKKNTTTPTPHDAAFWSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFEEPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGALEKALAIACQLQKMGMT
                     PEQIKQATGLSEAELKNITH"
     gene            13268..13513
                     /locus_tag="ACHKIK_003030"
     CDS             13268..13513
                     /locus_tag="ACHKIK_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084158.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKKGALEV
                     AREAACRLRDMGWTPERIQEATGLSGEELKKLFPDEQ"
     gene            complement(13544..13795)
                     /locus_tag="ACHKIK_003031"
     CDS             complement(13544..13795)
                     /locus_tag="ACHKIK_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO9084159.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(13829..14024)
                     /locus_tag="ACHKIK_003032"
                     /pseudo
     CDS             complement(13829..14024)
                     /locus_tag="ACHKIK_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14374..15222
                     /locus_tag="ACHKIK_003033"
     CDS             14374..15222
                     /locus_tag="ACHKIK_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO9084160.1"
                     /translation="MNQTLPTADLNTAGTTDIIPSMAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTYTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQELIDIWRKLSW
                     DYRTNNPCCLGKRIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNIGELFEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     VLPPRV"
     gene            complement(15308..15643)
                     /locus_tag="ACHKIK_003034"
     CDS             complement(15308..15643)
                     /locus_tag="ACHKIK_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO9084161.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            15875..16207
                     /gene="mobA"
                     /locus_tag="ACHKIK_003035"
     CDS             15875..16207
                     /gene="mobA"
                     /locus_tag="ACHKIK_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO9084162.1"
                     /translation="MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVST
                     LIRKTVLGKQINARIDEDFLKELMRLGRLQKHLFVEGKRTGDKEYAEVLVAITELANT
                     LRRDLMGR"
     gene            16219..18939
                     /locus_tag="ACHKIK_003036"
     CDS             16219..18939
                     /locus_tag="ACHKIK_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO9084163.1"
                     /translation="MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEI
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQK
                     AADLAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELEIKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIVQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPLWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLATAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYGLIQCRSLIEEMIADGDF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRT
                     TNVDRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTRTGKNVCTDYGDRVVFYHH
                     TDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFT
                     ITRPDVDLHRQRSEQYYREYIRQQTRLSVSHDDNYTLRQEKTWEPPSPGM"
     gene            complement(19160..>19450)
                     /locus_tag="ACHKIK_003037"
     CDS             complement(19160..>19450)
                     /locus_tag="ACHKIK_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HFO9084164.1"
                     /translation="QYDTDIESRANELWKKAVHTINNTTREERRVCYITLNRPEEEHS
                     DPEDIPSVKAILNTLLPLEILLPVAENNKSQTINNDHPQKSCGAQPTTDEKY"
BASE COUNT         4642 a         4658 c         5332 g         4818 t
ORIGIN      
        1 tgcggcttgg aaccccttag tattttttgt ctgtagtatc tatcccagca ataggtatat
       61 cctgttgcat caataaagtt gacttttgta tacaacatgc gaatttccct taatccggag
      121 ctattcgtat gataaaaaaa actcttcctg ttctgattct tctggcgcta tcggggagct
      181 tttctaccgc tgtagccgct gataaaaaag agactcaaaa tttctactat ccagaaacac
      241 tggatttaac tcctctgaga ttacacagcc ctgaatcaaa tccctggggg gctgattttg
      301 attatgccac cagatttcaa cagctggata tggaggctct gaaaaaagat atcaaagatt
      361 tgctgacaac ttcccaggat tggtggcctg cggattatgg tcattatggt cctttcttta
      421 ttcgtatggc ttggcacggt gccggaacat acaggacata tgatggccgg ggaggcgcca
      481 gtggtggtca gcaacgtttt gaaccgctga acagctggcc ggataacgtt aatctggata
      541 aagcccgtcg attgctgtgg ccagtcaaga aaaaatacgg ctccagtatt tcctggggag
      601 acctgatggt cctgactggt aatgttgccc ttgaatccat gggatttaaa acgctgggat
      661 ttgctggcgg aagagaagat gactgggagt cggacctggt atactggggg cctgacaaca
      721 agcctcttgc agataaccgg gataaaaacg ggaaacttca gaaacctctt gccgccacgc
      781 agatgggact tatttatgtc aatcctgaag gccccggtgg aaaaccagat cctctggctt
      841 ccgcgaaaga tatcagggaa gctttttcac gtatggccat ggatgatgag gagactgtgg
      901 ccctgatcgc gggagggcat acatttggta aagcacatgg tgcagcgtct cctgaaaaat
      961 gtattggcgc agggcctgat ggtgcacctg tggaggagca gggactggga tggaaaaata
     1021 aatgtggtac aggaaacggc aaatatacca tcaccagtgg cctggaagga gcctggtcga
     1081 catcgccaac ccagttcaca atgcagtatc tgaagaattt atataaatat gaatgggagc
     1141 tgcacaagag tcctgccggt gcttatcagt ggaagcctaa aaaagcggca aatatagttc
     1201 aggacgcgca tgatccgtct gtcctgcatc cgttgatgat gtttacgacg gatattgctc
     1261 ttaaagttga tcctgaatat aagaaaataa ccacccgttt cctgaatgat ccaaaagctt
     1321 ttgagcaggc attcgcaaga gcatggttta aactgaccca ccgggatatg ggaccggcag
     1381 cccgatatct tggtaatgaa gttcctgcag aatcatttat ctggcaggat cctcttcctg
     1441 cggcggatta tacaatgatt gatggtaaag acattaagtc gctgaaagag caggttatgg
     1501 atttgggtat ccctgcatct gagctgataa agacagcctg ggcttcagct tccacatttc
     1561 gtgtgactga ttatcgtggg ggaaataatg gtgcccgcat caggttacag cccgaaatta
     1621 actgggaagt taatgagcct gaaaaactga agaaagtact ggcatccctg acctcattac
     1681 agcgtgaatt taacaaaaaa cagtctgacg gaaagaaagt gtcgttggct gatttaattg
     1741 ttctttcggg taatgctgca atcgaagatg cggccagaaa agccggggtg gaacttgaga
     1801 ttccctttac tccgggaaga actgacgcct ctcaggagca gacggatgtt gcctcattca
     1861 gtgtactgga gccgacagca gatggattca gaaattatta ctcaaaaagc agaagtcata
     1921 tatcgccggt tgaaagcctc attgataaag ccagtcagct ggatctcacc gttcctgaaa
     1981 tgacggcatt actgggtggt ctgcgggtaa tggatattaa tacaaataat tcttcgttgg
     2041 gagtgtttac cgatacccct ggtgttctgg ataacaagtt ttttgttaat ctgctggata
     2101 tgtcaacacg atggagtaaa gcagataaag aagatacata caatggattc gatcgtaaaa
     2161 cgggagcatt aaaatggaaa gcatcctctg ttgatttaat cttcagttca aatcctgaat
     2221 tacgtgcggt ggcagaagta tatgcctcgg atgatgcgag aaataagttt attcatgatt
     2281 ttgttaaatc gtggaataaa gttatgaata gcgatcggtt tgatttaaac aataaatgat
     2341 aaaactgatg agggagggca cctccctcat gaaggagaaa ttatgcagaa aagcaataaa
     2401 gttataatca gctgtggtat ggcattatat tttttgtgtg gggggattac agtggcagaa
     2461 gaacttgatg aaagcatgca agttcttgca aggaatatga gtgtacttca gtcatcaatg
     2521 gataaaaagg agcttcttga agctcttgac agaatggatg atgctgtaga tgagtcaatg
     2581 aagcgtcttc ctgagagcat ttcaccggct gatgaagaag ggaagaatga ctatataaaa
     2641 gaacttcaaa agcttgaaaa agagattatg ctggcgaaac aaaaagtctt atcgggacag
     2701 ttatcagata tacccgattc ggtaaacaag atgaataaaa tcagagttga ggggcatgat
     2761 aaattcagat aaatcagttg gttgattcct gcgggacgaa tacatacgtc aggaacataa
     2821 ctttatggca gtgaaccgcc ccgggaatcc tggagactaa acttcctgag aaagaggtaa
     2881 acaggatgac taaaaatact cgtttttccc ccgaagtccg tcaacgggcg attcatatgg
     2941 ttctggaaag tcagggcgaa tatgactcac aatgggcggc aatttgttcc attgcccaaa
     3001 gattggctgt acaccggaga ctctgcgtgt ctggggacgc cagcatgagc gggataccgg
     3061 gagcggtgat ggtgggctca ccagcgctga acgtcagcgt ctgaaagagc tggaacgtga
     3121 aaaccgtgaa ctgcgccgca gtaacgatat ccttcgccag gcttccgctt attttgcgaa
     3181 ggccgagttc gaccgcctct ggaaaaaata atgccactgc tgctgcgtga gcagtacggg
     3241 gtcggaccgg tatgcagtga actgcatatt gccccgtcaa cgtattacca ctgtcagcaa
     3301 cagcggtatc atccttataa acgtagtgcc tgtgcacagc gcgatgactg gctgaagaaa
     3361 gagatactgc gcttatacga tgagaatcat caggtatacg gtggcgtaaa gtctgacgtc
     3421 agttgttaca ggaaggtatc agggtggcca gatgtatagt ggcgcgcctc atggcggtta
     3481 tgggacttgc cggtgttctc cggggtaaaa aggtccgtac taccgtcagc cggaaagccg
     3541 tttccgcagg cgaccgcgta aaccgtcagt tcgtggcaga acgtcctgac cagctgtggg
     3601 tggctgattt tacttacgtc agcacatggc agggcttcgt ctgtgtggcg ttcgatttag
     3661 ggcactaaaa ccgctgctgg tccctcataa taaaagaaga gcaggtaata gacttaacct
     3721 gctcttcttt tattattttt ttaacataga aagattgcgt ctggtttgtt cttcgaaatc
     3781 atcttttgaa gtgatgctct ctgatttgtt ttctgaagat gcccctatga tgttttttaa
     3841 cacacttata tcaattgatg tagatgtgta tcctgttgtt tgctctataa gtttcaccag
     3901 attttctgca gtgattttct cactgaaaaa accactgatt agcgttaaca gtcgggggtc
     3961 aacagaataa agagctgctc cgcaaataag agattgtctg tagaagtctc cccgcatttg
     4021 tatcgggaga gagtctattg tttgttgtgt caggttatcc tgcggataaa gtgtcgggtg
     4081 caggtatgcc acaactttct ttcttttttt cggatcactg ctttccatta cgcatgcacc
     4141 attgctttca ccaatgcaaa ctgaggttca tccaccttgt tcactttcag attcgggaaa
     4201 tattgcttta tgtgagggta aataagctcg gctcctccgc cagtgagata gattcggttc
     4261 attcctgaga atgttgaaat ttcctgcctg atgctttctg ccagagattt cacttccctg
     4321 tctattacat gagtaatgtt ttttatttca gctacatcat ttattaatgt ctggagatat
     4381 tcattatcat gtctgttttt tattaactca tcagcaataa aataattaca tggtgtggag
     4441 gctttcgtca gcgcattcat gacggtatga gtgatgcggg atgtcccgat ttctgaatac
     4501 ccacggattt cagaaatgcc ttcaaatgca ccgagaatta aaccacaatc cagagtggtt
     4561 cctccaagat cgataatcag gctgcgttca agtttgttta ctttatcttt ttttagtgat
     4621 tcaaatgcag ctggcaaaga ttcaggcatt acattaactt ttttaatttt aaatgtttcc
     4681 cctttattca gagatatctc acggagaaca tttgcctttt ttctttctat tctttcttca
     4741 tttggttgat tgtcattatc aaagaattcg gttacaggta atgtcactgt cagttctaca
     4801 tcctgtggct gaaggccgga tgtcagcagt gcatgatgta tggcaagcaa attggtggtg
     4861 ctgtattgat aactgacatg tgttgtacct atgacagcag tactgcccaa atcataggtg
     4921 tatttttttc catccacgac gtaattgtaa actttgccgg cattaaacag tcccgggttc
     4981 cagccttctt taaagctgtt tcctgacaga ctggttctta cctcgccatc ttcaagccag
     5041 gccagtttta cgttagttga accatcatcg catgatactt ttagcatttt ttcttgcctc
     5101 gttttgagtt tttatttatc tcaaattgag catcctttat ctagatgcat ctttttgagt
     5161 tttttgttgt tccttattgt gtctcatttt gggttcatgt caagcgatga ggctctttta
     5221 gagtctcttt gcattcttgt tgtgactcag tttgagtctc tatcgatttc tcattttgcg
     5281 tttcgaactg agacggaaac tacttaacca ggccgcatgt gagaggagaa ttcccatgcc
     5341 aaactggtgc tcaaatcgta tgcatttttc tggtgaaccg gcacagattg ctgagattaa
     5401 acgactggcc agcggtgccg tcacaccgct ttatcgccgc gccacaaatg aaggtattca
     5461 gctgtttctg gccggaagtg ccggacttct gcagaccact gaagatgtgc ggtttgaacc
     5521 atgccccgga ctgacggctg ccgggcgtgg cgttgtatcg ccggagaata tcgcgttcac
     5581 ccgctggctg acatacctgc agaacggtgt actgctggat gagcaaaact gcctgatgct
     5641 gcatgaactc tggctgcaga gcggtactgg acagcgtcgc tgggaaggat taccggacga
     5701 tgtcagggat accatcaccg cacttttcac cgcaaaaaga ggtgactggt gtggcttctg
     5761 gagtaacgag gatgtatcgg tgtggtggaa ccgtctgtgt gacaacgtac tgccggaaaa
     5821 aaccatgccg tttgacctgc tgacggttct gccgacccgc ctggatgttg aagtgaatgg
     5881 ctttaacggt ggtgttctga acggtgttcc ttctgcatat cactggtata cggaacagta
     5941 tggcgtgaag tggcctgtgg ggtatgaggt gaatatcagt agtcagggag acaacttcat
     6001 tcaggttgat ttcgacacgc cgtggtatca gccggaaagc gacgttattg cagaattaag
     6061 ccgccgtttc agctgcacac tggagcactg gtatgccgaa cagggctgca atttctgtgg
     6121 ctggcagcga tatgagcgtg gcgagcttgt tgatgtgctg tggggggagc tggaatggtc
     6181 acatccgaca gatgatgacg agttgccgga agtcaccgga cctgcgtgga tagtcgacaa
     6241 ggtggcgcat tatggcggat gaagtatgac ggagacgggc gggcaactgc ccgtttcttt
     6301 tccggtaaag gatgtcgccg tgctcctctt tttactgcgc cggctgacgc ggcgcggcat
     6361 aaacgctgtc tgtggtcagt gtcccgcgtc cggcgggcac gggacgggcg cttttaccgc
     6421 tcccggctgg cgtcggtgac agtgtacgcc agcccgtcgc ccttttctga tgaacgccct
     6481 tcaagccgct ttcgcggcat agccttgccg tcaaaaagac gaaggctgcg gtattccacg
     6541 gtcggcctga cccgttaccg gacgcggtga acagcccaca ggcagcgggt aacgggcacc
     6601 acaggggtgc cctcccggtg ccctctgtaa gagaaggagt tttgtatgtc ccgttttgtc
     6661 ctcggtaact gcatcgatgt tatggcccgt attcctgata acgccattga tttcatactg
     6721 actgacccgc cgtacctcgt cggtttccgt gaccgttccg ggcgcaccat tgccggtgac
     6781 gttaatgatg actggctgca acctgccagc aatgagatgt accgcgtact caaaaaagac
     6841 gcgctgatgg tcagcttcta cggctggcac cgtattgacc gctttatggc ggtctggata
     6901 cgcgccggat tcagcgtggc agggcacctc gtgttcacca aaaactacac atccaaatct
     6961 gcgtatgtcg cgtaccgcca cgaatgcgct tacatcctgg caaagggcag tccggcccgg
     7021 ccccgtaacc cgctgccgga tgtgctgggc tggaaatatt cgggcaaccg ccatcacccg
     7081 acggaaaagc ccgttaccag cctgcaaccg ctgattgaga gcttcacaca cccgaactca
     7141 attgtgctgg acccgtttgc aggcagcggt tcaacctgcg tggccgccct tcagtccgga
     7201 cgccggtata ttggtatcga gctgcttgaa cagtatcacc gtgccggaca gcaacgcctt
     7261 gccgccgtgc aacgggccat gcagcagggg gccgcaaatg ataacaggtt tgaaccggag
     7321 gcagcatgat gaattacagc ggatttgaag atatgcgtga acaggtggct gaactggcca
     7381 acaccatgtg cgacctgcgc acaaccatga atgagatgga gcagcggtac agcgatgcag
     7441 acaccctgcc ggaacgtctg gtgcgtcaga cgctctttcg tgccagccac ctgtttatga
     7501 aggcatataa cgaaatactg gaactggatt actgcttcaa agattgaggg gaaagagtat
     7561 gtacggaaca tgcgaaacgc tctgccgggc gctggcagca aagtatccgg gagacacgcc
     7621 gttgatgctg gttatctggt ccccggaaga gattcaggcc ctcgctgacg gaatggatat
     7681 ttccctggcc gatcatgaaa tcaggaccat cctggcgcgc ctggaggaca tcccggaaga
     7741 ccagcggact gagtccggta tttcttcctc cgcggcgatg gagattatca gcaacgtgag
     7801 cgaaaaccgc caggtgaccg tccctgctga actgctggcg tccctgattc agaccgctga
     7861 acaggcattg tggaagcgtg aatgggccgc ccgtgattac ggcctcgccg tcccggaatg
     7921 cgttacccgc cgtcaggcgg tggttaatca ggcccgcatc ctgctgaaaa acaacacgca
     7981 cgaaaacgac tgatgttatc gccgcctccg ggcggcgatt cagggagacc atgaatcatg
     8041 aataaaacgc tgaatgcact gatttgtcgt catgcccgcg acctgctgct ggcgcagggc
     8101 tggccggaag agacggatgt tgaccagcgt aacctgaagt atccgggctg gatcagcatt
     8161 tacgtgcggc tggatgcgcc ccggctggcg acgttactcg ttaaccgcca cgatggcgta
     8221 ctgccgccgc acctggcctc cgccattcag aaactgaccg gaaccggggc ggaacttggt
     8281 ttcacgccag cccacgaacg tttccacctg gcattatgtt cgccgggaga cgtctctcag
     8341 ctctggatgc tggttctggt gaattgtggt ggacagcctt tcgccgtcgt tcaggtgcaa
     8401 catatcttca cgcctgtcgc tatcagtcac acgctggcgc ttgccgcgac acttgatgcg
     8461 cagggataca gtgttaacga catcatccat atcctgatgg cagaaggagg tcaggcatga
     8521 gcgcacgttc acgggcactg attcccctca gcgcagaaca acaggccgcc atgcaggctg
     8581 tggctgtcac agaacaacgt cgtcgtcagg gacgcacact ttcagcatgg ccttatgcca
     8641 gcgctttctt tcgctgcctg aatggcagtc gccggatttc gctgaccgat ctccgctttt
     8701 ttgcccctgc gctgacgaag gaggaatttc atggcaaccg tctcctgtgg ctggctgccg
     8761 tggataaact gattgaaagt tttggtgaag tctgtgttct tcccctgcca tccgatgcgg
     8821 ggcatcgtct gttcccgtcc gttccttttc gtgaaggtga gcgtcgtcgt cagaaaacca
     8881 cgctgacaga gcagaaatac agccgccagc gggaacgtga ggcagaacga cgggaactgg
     8941 aataccagac atgttttgct caggcgcaga ttgaccttgc gtttcatact cccgccacgg
     9001 tcggaagctg gttgtcccgc tggtctggtg ttgttgagga gcatgatctg gaaacgattt
     9061 tctgggggtg gtgcgggcgt tttccatcac tgtcatcatt tgaccggttt ttctggcagg
     9121 aggaaccact ctggcggctg atttttgaag ccggtgaggc cggtcgtggt gcaccggtac
     9181 aggtacgtgc acttgagcag tggatgatcc cgaacaagct ggagaacgca atatgatgaa
     9241 atcagacgaa caatatcagg ttcccgtgtg gatgcgacct ctgttgccgt tgctctgcaa
     9301 taccggtggt aatgatccgg aggaacttct gaatgataca gaaaccaccg ccagtgcgaa
     9361 tattgtccgt tatgtactga tagttgcagt gcggtcgcag attggatctg ctgcagctac
     9421 tgtacaggaa aggactgttg cgcacagaga taccaggtgg cttttcaccg gaagaagcgc
     9481 aggaactcct ggataatctg gtgcgcagcc atatcagcaa ggcgctgtcg ggtgagcgaa
     9541 tggcagcccg tgacagatat gccgagctgg cctggattcg ccagcaactg gtggatgccg
     9601 cctggtttgt ccgtgccaca ctggaagcgc atggtacgag cgtcggaaat gagagtccct
     9661 ctgcacttcc agagacaatg ccggacatac agacacggga actggtgatg ctgatcaaac
     9721 gactggcatc ttctctgaaa gcggtgaaac cggacagtag cgtggtgcgt gcggcgcagg
     9781 actggctgtg cgacagaaaa cttgtggata tcacagatat tctccggtga accaggcatg
     9841 tatatttttc gcagggacac gccgatcatg atcgtttcat atcaggggtg gtctggaggt
     9901 catgcggcgt gtcctctgca ctcgccggaa taaggaagtc gccggcggat ccgcttttac
     9961 ccggccatgc ggggcatggc cttgtgggtt ttcagttatg tggcctcagc gtcgtgtgcg
    10021 ggctgtgccg tgcctccatc gtagccgggc ttctgcgggc gggatgcaag gtaagatgaa
    10081 ccgctgcggt cacccggtcc ctccttcccg tctgccgtga ttttccggtc gttcacccgc
    10141 tcagatttcg cggtctcacc ctccaactcc cgtcagccgt gcgcgtcggg ctgcggcttc
    10201 ccttgcgccc ttgcatccct gcaggcccgg caaagatgga ggcacggcac cgcccggtgt
    10261 cgcagaacat gtgactatgg aggattcggg aatgtctgtt gttgcacctg ctgtatacgt
    10321 tggaacctgg cataaataca actgtggaag catcgctggc cgctggtttg acctgaccac
    10381 gtttgatgat gaacgcgact ttttcgccgc ctgccgtgct cttcaccagg atgaagccga
    10441 tcctgaactg atgtttcagg attatgaggg gttcccggtg aatatggcct ccgaatgcca
    10501 tatcaactgg gcctgggttg aaggcttccg ccaggcacgg gatgaaggct gcgaagaggc
    10561 ttatcgtctc tgggtggatg ataccggtga gacggatttt gacaccttcc gcgatgcctg
    10621 gtggggcgag gctgacagtg aggaggcttt tgcggttgag ttcgtcagtg ataccggcct
    10681 gctggctgac gtgccggaga cggtggcgct ctattttgac tatgaggcgt atgcgcggga
    10741 tttattcctg gactccttca cctttattga cggtcatgtg ttccgtcggt gatttactcc
    10801 cccgctccgg cgggggtatg gcctgccggg ccgccggaaa acccaggcag gcttacgcct
    10861 gcctgaaata caggagggcc gcgcctgcgg cgctgccctt cctgcccgtc atggtgtgag
    10921 gcatttttct gttttgtcgt ttctgttgtt ctgatgcccg gaggcgggga cacccttcgg
    10981 ctcccggctg cctggcggca tccggtcgtc ggcccgactc cgacaggcag ctgctggcgc
    11041 acccgccagt ctccgccgaa cccgctggcg cggtttcggg ctgccgctac ggtccgatgt
    11101 ctgaaaaacc aggcctgcag cgaccgttcc ggtcgcgccg cccctgtcgc gccggaaacg
    11161 gcgctccctg cacacctgac ggtgtgtaag gccactgcgc ctgatggcgc agtcaaccct
    11221 tctgccgcca cccctgcggc ctgtttcccg ctgtggactt tccgggggct gaagcggcca
    11281 caacacgggg cagccgtcca ggggaagatg taccagccac aaaacttttt gccgcaggcg
    11341 tcaaaaactt ttgcgtctgc ccccggacaa ctgccccgtg ccgtggcagt cgctgcccca
    11401 tcggaaaggt ccactgcggg gagaaacccc gaagaagtgg acggcagaag gggctttcgc
    11461 ccctccgggg gagcaaccgg tcggcccggc gacctgattc agcagaagga gacgttttca
    11521 tgacagtcag cagcacgatt tccgtttttt gccgcgacgg ggtgttccgt accgtttact
    11581 gccacctgca cggggagccg acctggaacg gtcgcatcct gcatacccac tatgccaccg
    11641 gtcagctggc cgaagccctg gttgaacacg gagatatccg ttgcctcggt ccccgttgcg
    11701 acaaatccgc cgggcatacg cttcagaacc cggtggacgg tgtgacggct tattacggac
    11761 gtgacagtgg tttccggatg gacagtgagg cgcgtgagta ccgttctttc atggaggcta
    11821 ttgccactga aagcactgaa gaggtgcgct tccattatgt gttcatcgac ggctactgga
    11881 aggtgatgta ccgcacgccg gaaggctgga agatgaaagc gctcgcgctg gcactgcgcc
    11941 gctgtccgaa atgaaaaaag gcagggcgat aaaccctgcc tttctccgcc ggcgcttccc
    12001 cgtacggaag tccggcatct caatcactat ctatggagat tatgccatga gaccatccat
    12061 tatcttcgca accgccgagt atgtaaagcg tctgcgtgaa gagtgcctgc gggagaatat
    12121 acccctgcac cgccataccc gcgtcagacg tcaggagctg gcacaggatg agattaaccc
    12181 ggacgtcctg gcgatgagcg gtcatatcgc cagacgctgc agtgagcaga agcgggtacg
    12241 tatccccgcc atgaaagtca gcgaatgggg ccacctgctc cgcgcgcttg aaattgagcg
    12301 ggcctgccac tgattaaccc ttgccgtctg cctgtgcagg cggcaggata cgttcagaca
    12361 tctcaccagg aggaaccgtg agtaagaaga aaaacaccac aacgcccacg ccgcatgatg
    12421 ccgcgttctg gtcgttcctg gcgaatcccg acgtcgccag agattttctg gaactgcatc
    12481 ttccggcgga gtaccggcag ttgtgcgacc tgtccacgct gaagctggaa cccgccacct
    12541 ttgttgagcc ggacctgcat cagtacgcca gcgatatcct ctggagtgtg aaaaccaccg
    12601 ggggtgaaga cggctatgtt tatacgctca tcgagcacca gagcaccgaa aatctgtaca
    12661 tgcctttccg catgttacgt tacagtgtgg cggcgatgca gagacatctg gagcagcaca
    12721 aaacattgcc actggtgatt ccggtgctgt tctatcatgg tgagcgcagc ccgtacccgt
    12781 acagcatgaa ctggctggac tgttttgagg aaccggcgct tgcggctaaa atatacacaa
    12841 agccgtttcc gctggttgat atcactgtcg ttgatgacaa tgaaatcatg aaccatcgcc
    12901 ggatggccgc actgacgctg ctgatgaagc atatccgcca tcgtgacatg atggagctgc
    12961 tggacaaact cccgcaggtc atggtggaaa tttcagacga gcaggtgcgt gttctgattc
    13021 attacatcgt taacgcaggg gactctgtat caccggaatt tatgcgggcg ctggctgagc
    13081 gtctgccgca gcatgaggat aaactgatga ctatcgctga acgtcttgag caaaaaggtc
    13141 gccaggaagg agcgcttgaa aaagccctgg ctattgcgtg ccagcttcag aaaatgggga
    13201 tgacgccgga gcagattaag caggctaccg gactttccga agctgaactg aagaacatca
    13261 ctcactgatg agaaccacaa agcccggcaa aatgccgggt ttttttgtca gcaggagcag
    13321 agcaaacagc atgacgattg cagaacgtct tatacagaaa ggtttcgacg aaggttttaa
    13381 gaaaggtgcg cttgaagtgg cgcgggaagc agcctgccgg ctgcgggata tgggctggac
    13441 gccggaacgg attcaggagg caaccggact ttccggtgaa gaactgaaaa agctgtttcc
    13501 tgatgagcag tagcctggca ttctgccagg ctgtgaatca cgctcacaca ttccctgccg
    13561 tttcccccag ttcattttcc agacgcctga tgatatctgc cttgcgcatc ccctgcatgt
    13621 gaggcagcag atcacggact gaaagtgcct gggtggtcct ccagacggtg acatccacct
    13681 gttccgcatt ttcccaggcg tgccatgttc tctgtgtcac gccaaattct ctggccgctt
    13741 tttcgcgtga ccagagcatg ctttttcgcc agattcgcag ttcccagccg gtcataaaac
    13801 acctttgaaa aagtgaaaaa acttcacttt attttatcat cataatcacc aggtcagcgc
    13861 gataaataag agtgaaaaag tgaagaaatt tcacttttta gttgtaggtg tcgtgctgtt
    13921 ttctctgttt tcacctgtgc gggagtggtg cgccagtgac caccacatgc cattgttgtg
    13981 ctgtcgtttt tcttcagtcg cgccggatgc ggcgctccct gcacacctga cggtgtgtaa
    14041 ggccactgcg cctgatggcg cagctgtctc tgcctgtctg ccgccgcact gcggcccgtt
    14101 ctccgcagac ggactttccg ggggtacata cacgaccaca tcccgtttca gtcgtccagg
    14161 ggagagatgt accagccaca aaactttttg ccgcaggcgt caaaaacttt tgcgtctgcc
    14221 cccggacaac tgccccgtga cgtggtgagc gtcccccatc ggaaaggtcc actgcgggaa
    14281 gaaccccctg tgaagtggac ggcagaagga gcttcgctcc tccgggaaat gaccaggctg
    14341 acccggcagc cgtgacgatg aggagacaat atgatgaatc agactttacc cactgctgac
    14401 ctgaatactg ccggcacgac agatattatt ccgtctatgg ctatcgaccg catcatcgcg
    14461 cagcgtaacg aaggtattgc actgttcatg caggcgatgg aatgcctggc gacagcgcgc
    14521 aggattctgc tcgatgcttc cggtgacatt tttctctacg ggtttgaaga ctgcgtgact
    14581 gactccgttc gatgcatgga taaaccggaa gaagcaaaaa agaatatcac ccgtcttgcc
    14641 gaccggaaaa tctgggatcg cctgatgact tatacgggca tgtacacctt catgagttca
    14701 tgccagcgtg atgagtggaa cagccagctg atgagcgaca cctgtcctga aatcaccctg
    14761 gacaatgtac tggcaacatt ccgccatctg aatgccagta agatgcagac atttgaacag
    14821 gaactgattg atatctggcg gaaattgtca tgggattaca gaaccaataa tccctgctgt
    14881 ctgggcaaga gaatcattat tgaaaacctg ctgtaccgct ggagtaacgg gcgtgtgacg
    14941 ctggactgca gcggacggga ggcactggat gacctggtac gtccgtttta tctgctggag
    15001 ggacgcaacg ttcctgactt caggaacagc atcggggcgc agtatggtga atttctcggg
    15061 aacggcgaca atatcggtga gttgtttgaa ggggaatatt ttacggtgcg tggctaccag
    15121 aaagggaccg tacacattgt ctttaagcgt cctgaccttg ttgaaaaact gaatgatatt
    15181 attgcacggc attatcctgg tgtattgccg ccacgagtct gattaaacag aaaagccctg
    15241 gtatttatgc cggggctttt ttttctgtaa tcatcataat ctcagaatac gatgtgacat
    15301 tgtcacatca attgttgttc atatcgatgc catctttcct gcctgaatct gtctttttag
    15361 gtgctgtttt agaggaagaa cggcgggatg gttttttcga ttctgcttcc cgaataatgt
    15421 cacgaagtgc cttctctcct acttcatacc ctgcattttt gagcgtgtca cgaacatctg
    15481 ccagtgtata accctttgtc cttaccagga caatgatgtc atcacgaatg gcatcaagaa
    15541 aatcacgcaa tgtttttcgt tgagcggtga ggtccggcaa ttctgataaa gcagcttttg
    15601 ccagctggat gtcatcatct gagtaaaatt ttttagaagc cattggcact ttttctccgt
    15661 ttcatgttga agtctgattt tatcatcaaa aactgcctcc tggcagagct gcgccccgcc
    15721 tctgccgtct gcataagact atgatgcaca aaaataacag gctataatgc ctgaaaaacg
    15781 ggacaggatg tgtaatcgta taaccgcgca cgcacggcta tacgattacc acctggtcag
    15841 ggcttagccc cgacaccccg gtaaggagca gaccatgagt gagaagaaaa cccgaagtgg
    15901 cagtgagaaa cggcaaaaga atgtcctcat tgctgttcgt ttttcaccag aagaggctga
    15961 gattgttaaa gaaaaggcag aaaaaaacgg actgacagtt tcaactctga taagaaaaac
    16021 tgtgctggga aaacagataa atgcccgcat tgacgaagat tttttgaaag agctgatgcg
    16081 tcttggtcgc ctgcagaaac atctgtttgt ggaaggaaaa cgcacgggag ataaagagta
    16141 tgcagaggtt ctcgttgcta ttacagagct tgctaatact ctgcgaagag atttaatggg
    16201 acgctgagat aatcgctgat gaatgccatc attccgaaaa aaagacgcga cggaaagtct
    16261 tcttttgaag acctgattgc gtacacctca gtgcgggatg atgtgccgga agatgaactc
    16321 aggccagaca cggaaatgaa agctgagatt ccgcaccgga accgcttcag acgtcttgtt
    16381 gattatgcaa cacgtctgcg taatgaaaaa tttgtttccc tgattgatgt gatgaaagac
    16441 ggcagtcagt gggtgaattt ctatggtgtg acctgctttc ataactgttt gtctcttgaa
    16501 tctgcggctg aagaaatgca gaaagcagca gaccttgctc atttttccaa agatgataca
    16561 gatcctgttt ttcactatat actctcctgg cctgcgcacg aaagtccgcg ttctgagcag
    16621 ctttttgact gtgtgcgtca caccctgaaa tcccttgagc tgtcaaaaca tcagtatgtg
    16681 gcagctgttc atacagacac ggataacctg catgtacatg ttgcagtgaa tcgtgtccac
    16741 cctgaaaccg gctatatcaa ttgccttccg tggagccagg aaaaactcag tcgtgcatgc
    16801 cgtgaacttg aaattaagca cggattcgcg ccggataatg gctgctttgt gcatgcaccg
    16861 ggcaatcgta ttgtgcgcaa aacagcgctg gtacgtgagc gccgtaatgc ctggcgacgc
    16921 gggaaaaagc agacgttcag ggaatatatt gttcagatgt cgattgccgg gctgcgtgaa
    16981 gagcccgcac aggactggtt atctttgcat aaacggctgg ccagtgatgg tctgtatatc
    17041 acgatgcagg aaggggaact ggtggtgaag gatggctggg acagagcgcg ggaaggtgtt
    17101 gcactcagtt cgttcggacc gttatggaca gctgaaaaac tggggcggaa actgggggaa
    17161 tatcagcctg taccgacaga tattttcagt caggtgggga cacccggtcg ttatgatccg
    17221 gaagccataa acgttgatat ccggccggaa aaagtggcag aaacggagag tctgaaacag
    17281 tacgcctgcc gtcattttgc cgaacgctta ccggaaatgg cgagaaatgg tgagctggaa
    17341 agttgcctgg atgtgcacag aactctggca acggctggat tatggatggg gatccagcac
    17401 gggcatctgg tactgcatga tggatttgat aaacaacaga caccagtacg tgcagacagt
    17461 gtgtggccgt taatgacgct ggattatatg caggatctgg atggtggatg gcagcctgta
    17521 ccgaaggata tttttactca ggttataccc ggagaacggt tccggggacg taatcttggt
    17581 actcaggctg tcagtgatta cgaatggtat cgtatgcgga tggggacagg tccacaggga
    17641 gcaataaagc gcgaactgtt ttctgataag gaaagtctgt ggggatacgg ccttattcag
    17701 tgcaggagcc tgattgaaga gatgatagct gacggggatt tcagctggca ggcatgtcat
    17761 gagatgtttg cgcgtaaagg gcttatgctg cagaaacagc atcatggtct tgtgattgtg
    17821 gatgcgttta atcatgagct gacaccggtg aaggccagca gtatccatcc tgatttgacg
    17881 ttatcccgtg ccgagcctca ggccgggcca tttgaaattg cggctgcaga tattttcgaa
    17941 cgggtgaagc ctgaatgtcg ttacaatccg gaacttgctg ccagtgatga agtggaacca
    18001 ggcttccggc gcgatcctga gattcgtcgt gagcgccgtg aagcccgtgc agctgcgcgg
    18061 gaagatttgc gtgcgcgtta tcttgcatgg aaggagcact ggcgtaaacc cgacttacgt
    18121 tacggagaac ggttacggga aattcatgct gcctgccgtc ggcgcaaggc gtatatccgt
    18181 gtgcagttcc gggatccaca attgcgtaag ctgcattacc atattgcgga agtgcagcgt
    18241 atgcaggcgc tgatcaggct gaaggagagt gtgaaggaag agcggttgtc actgattgcg
    18301 gagggtaagt ggtatccttt gtcctaccgg cagtgggtgg aacaacaggc ggctcaggga
    18361 gacagggctg cagtatcgca gttacgtggc tgggattatc gtgatcgtcg ctcgcgtaat
    18421 aaggataaaa gacgcacaac taatgttgat cgttgtgtcg tactctgcga acccggagga
    18481 acgccacttt ttaataatgt tgcaaaactt gaagctcgtc ttcagaaaaa tggaagtgtt
    18541 catttcaggg atacgcggac aggaaaaaat gtctgtacgg attacggaga tcgggttgtg
    18601 ttctaccatc acacggaccg aaatgaactg gcagagaagc ttgatttaat tgcgccagtt
    18661 ctgttcagcc gcaacggaaa gcttggtttt gagccggaag gttcatacca gcagtttaat
    18721 gatgtgtttg cagaaatggt ggcctggcac aatgcagcgg gaataaccgg aaacggacac
    18781 ttcaccatta ctcgtccgga cgtggatttg caccgtcaac gcagtgagca gtattaccgg
    18841 gaatatatca gacagcagac ccgtttatcg gtgagccatg atgataatta tacgttacgg
    18901 caggagaaaa catgggagcc accatcacct ggaatgtaag cccggataag tgatggtgca
    18961 aagattcagc ccaaaaacgg caggaacctt cgtcaataag ggcgggagta gtcactgaat
    19021 tgagtttata cccggttaac tctgaattaa gggccgtgtc tgcggccctt atccagtgcg
    19081 ccgtaaaacc ccggacttag tgaacagggt tttacagtct gattgacaga tatggtcacc
    19141 agaaaatatt tgtattcctt cagtattttt cgtcagttgt tggttgtgct ccacatgatt
    19201 tctggggatg atcattattt attgtctgcg atttattatt ttcagcgacc ggtaacagta
    19261 tctccagggg gagcagggta ttcagaatcg ccttcactga aggtatatct tcaggatcag
    19321 agtgttcctc ttccggacga ttcagtgtga tataacatac ccggcgttct tccctggttg
    19381 tgttattgat agtgtgcaca gcctttttcc acagttcgtt tgcccgggat tcgatatctg
    19441 tatcatattg
//
LOCUS       DAZGJW010000084        19336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZGJW010000084 DAZGJW010000000
VERSION     DAZGJW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.084"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..733)
                     /locus_tag="ACHKIK_003038"
                     /pseudo
     CDS             complement(1..733)
                     /locus_tag="ACHKIK_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1313..2314)
                     /locus_tag="ACHKIK_003039"
     CDS             complement(1313..2314)
                     /locus_tag="ACHKIK_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO9084165.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            2421..2717
                     /locus_tag="ACHKIK_003040"
     CDS             2421..2717
                     /locus_tag="ACHKIK_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO9084166.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(2851..3276)
                     /gene="arsC"
                     /locus_tag="ACHKIK_003041"
     CDS             complement(2851..3276)
                     /gene="arsC"
                     /locus_tag="ACHKIK_003041"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO9084167.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(3289..4578)
                     /gene="arsB"
                     /locus_tag="ACHKIK_003042"
     CDS             complement(3289..4578)
                     /gene="arsB"
                     /locus_tag="ACHKIK_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO9084168.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(4632..4985)
                     /gene="arsR"
                     /locus_tag="ACHKIK_003043"
     CDS             complement(4632..4985)
                     /gene="arsR"
                     /locus_tag="ACHKIK_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO9084169.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            5725..5808
                     /gene="dinQ"
                     /locus_tag="ACHKIK_003044"
     CDS             5725..5808
                     /gene="dinQ"
                     /locus_tag="ACHKIK_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO9084170.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(5862..7214)
                     /gene="gorA"
                     /locus_tag="ACHKIK_003045"
     CDS             complement(5862..7214)
                     /gene="gorA"
                     /locus_tag="ACHKIK_003045"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO9084171.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(7286..8128)
                     /gene="rlmJ"
                     /locus_tag="ACHKIK_003046"
     CDS             complement(7286..8128)
                     /gene="rlmJ"
                     /locus_tag="ACHKIK_003046"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO9084172.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            8331..10373
                     /gene="prlC"
                     /locus_tag="ACHKIK_003047"
     CDS             8331..10373
                     /gene="prlC"
                     /locus_tag="ACHKIK_003047"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO9084173.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHEEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10381..11133
                     /gene="rsmJ"
                     /locus_tag="ACHKIK_003048"
     CDS             10381..11133
                     /gene="rsmJ"
                     /locus_tag="ACHKIK_003048"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO9084174.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(11182..12651)
                     /gene="dtpB"
                     /locus_tag="ACHKIK_003049"
     CDS             complement(11182..12651)
                     /gene="dtpB"
                     /locus_tag="ACHKIK_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO9084175.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            12817..12870
                     /locus_tag="ACHKIK_003050"
     CDS             12817..12870
                     /locus_tag="ACHKIK_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084176.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(12969..13403)
                     /gene="uspA"
                     /locus_tag="ACHKIK_003051"
     CDS             complement(12969..13403)
                     /gene="uspA"
                     /locus_tag="ACHKIK_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO9084177.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            13794..14129
                     /gene="uspB"
                     /locus_tag="ACHKIK_003052"
     CDS             13794..14129
                     /gene="uspB"
                     /locus_tag="ACHKIK_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO9084178.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(14374..15873)
                     /gene="pitA"
                     /locus_tag="ACHKIK_003053"
     CDS             complement(14374..15873)
                     /gene="pitA"
                     /locus_tag="ACHKIK_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO9084179.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            16105..17307
                     /gene="yhiN"
                     /locus_tag="ACHKIK_003054"
     CDS             16105..17307
                     /gene="yhiN"
                     /locus_tag="ACHKIK_003054"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO9084180.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QILKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(17623..18675)
                     /gene="yhiM"
                     /locus_tag="ACHKIK_003055"
     CDS             complement(17623..18675)
                     /gene="yhiM"
                     /locus_tag="ACHKIK_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO9084181.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            19058..>19336
                     /locus_tag="ACHKIK_003056"
     CDS             19058..>19336
                     /locus_tag="ACHKIK_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084182.1"
                     /translation="MKAIDNIFKYVPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNDRSHQMEKVWFYNSEPSIDIQ"
BASE COUNT         5111 a         4700 c         4836 g         4689 t
ORIGIN      
        1 cagtgaaatt tatattaagt agatcttcga aataaaacct tgacaaggtt agaacgaacc
       61 cggacaggtt ctgtcctgtc atattgaaat caataaagtt ggttccggca aagactttca
      121 tcctgttttt attttcactg tgctcaatat ataacttcgt cattttttga tacaggcttt
      181 gaatatcttt tatattagtt ggtaagtcag ctcgttgttc agcaataaaa ttagtatctg
      241 aaaacatggt ttggacactg aatgttatgg gtattccctc ttctacgcta attttagtta
      301 tgattgcctt gtcgggttta ggattagcga caacaatgat tcgacattca gcacttttgt
      361 ttttttcata tctaatgtta ttaatctgat taagtattga ttgtgtctgt tgcgtaagtt
      421 gtgtcgaact tatcgtttca atgttagtaa taagacttac aagttgagcc acaacttttg
      481 agtagtctgc atttgtatat gtagtgttcg tgtggcacgc attaatttgt tgaagttggt
      541 gttgcgctaa atcatattga tcagagatat atccttctcc agagaatgtg gaattaatgg
      601 gttattgttc ttcccaatta tcgcaaggcc acttcggtgt gtctggcgtt ttagcattat
      661 cggtatttgt tgtgcagtta taaatatcgc cgtgatatgt atttattata cctggggtaa
      721 agtcaattga cataaaattc cttattcatc atttgtttta tgctaagatg tttaaattgg
      781 ataattagaa caggaatgga ttttcttcta cacacaataa atataatgcg ttatatcttc
      841 ctgatataat tacctttaaa ttccctttca tttccacccg gtaatatttg tgtataaaaa
      901 aaggcagtga tagtatcgta aagggaaacg ttaacaacta tcaaaaagcg ctcattttaa
      961 aaagttattt tgttctccga tagtgtgtga agtaatattt ttgtgatgtt ttagatgtgt
     1021 aataatgaac caggttaaag tgtatttaaa aatgtgttgt attagcgact gaaatctgcc
     1081 ttttaaaaag gaggagggta ctgagacaaa gagtcattgc ttctccgata aaatgtcaga
     1141 gaagcatgat tacgtggtta ttgtctgttg atgacttgtt gaccgagccg tcatcattct
     1201 caatgcatta gttaaatcat ttgtgatttt taaggattat cctgtctatg attttctact
     1261 tagaaggata tggcactaac tatgcatctt atagcttact gctggcatct atttaaaata
     1321 gcaattgagt caaatagccc atcaagatag ccatacctaa tataaccgtc aggaatgcag
     1381 ctatcatcgg cattctgaac attgatttaa gtaatatcac ttccgtcagg cttgcgccgg
     1441 cactgccgat gattaatgcc attaatgctc ccagacccat tccttttgtc atcagaacag
     1501 atgccagagg gataactgcc tcagcccgga tatacagcgg aataccgaca acggcgctta
     1561 atgggatggc gaagggatta tctgctcctg catgagcggc aatccacgct gagggaataa
     1621 agccataaat aaaagatcct attaatactc ctaacaaaag atatggcagt acatctttaa
     1681 actgttgcaa cgcatctttg gtggctagtt ttatgggatt aatacttact acagctttgg
     1741 tactgcaaca ggtaactacg gggttctcaa tggctttagc cgctggacta caacaactca
     1801 cttttatggg cgtatatgtc gttcccggcg aagtttttgc tggagcacaa caatttgctg
     1861 atgagctttt actggcaatg atatgacgtt caaaccccag agaatccagg ataatactgg
     1921 caagaacgga gacgccggct gcgataatcg cgtacaacaa ggtgactttc caaccaaagg
     1981 tcatccacat tagtccgacg ataatgggat taagtaatgg tgaaacaaat aaaaaagtga
     2041 gagtcggacc aaacccggct ttcgctgaca acaatccacg taacatgggt attgtcgagc
     2101 aactacagaa cggggtaacg gctcccaaca gagcagccag gagatagcct ctcccttttc
     2161 tggcccccat catctgctgg attttatggt ctggcacctt ttgtcttatc aggctgacac
     2221 cggcactaat cactataaac agcaacgaaa gctcgacggc gagaaacaca aacatctctg
     2281 cggcatcttg cagcatcgca agccaactac tcatacgtaa cctcattgga taaatctata
     2341 tttccagaat aatcgaaatg aaattcagat caagatattt ctggtattat cgaaatatca
     2401 accagacaga ggggtgtcat atgcagttgg aagaagtcgc taaagcgctg aaagagctag
     2461 gtcacccgac ccgtttgttc atatttaaac atcttgtcaa agccggggaa caagggttgc
     2521 cggtcggtga gttgcaaaag cagcttggga tacctggctc cacactcagc caccatatat
     2581 cagcactggt atcggtagga ctggtcaaac aaaaccgtga gagtcggacg ctgatgtgtg
     2641 tttcgcaata cgcaatgctg gaagcgataa ttgaattctt acgcgaggag tgctgcgtaa
     2701 atagcaaaat tgcctgatgc gctacgctct acatatttca tgccatatat tgaatttgca
     2761 cggttttgta ggtcggataa gacgcgttag cgtcgcatcc gacataaaca aagcgcactt
     2821 taccaactat cggcagtgga tatcgccgct ttatttcagg cgcttacccg cttcatcaac
     2881 cactttctcg ccatcttcct tggagaatgc gcctttttgc gcatctggca gaatttccag
     2941 caccacttct gaagggcggc acaggcgagt tcccagcggc gtcaccacaa tcgggcgatt
     3001 aatcagaatc gggtgctgaa gcataaagtc gattaaccga tcgtcagtaa atttatcttc
     3061 cgcaaggccc agctcctcat acggttcgac gtttttacgc agcagcgcgc gtacggaaat
     3121 ccccatatcg gcaatgagtt tgaccagttc atcgcgcgtt ggcggagttt ccagataatg
     3181 gataatggtc ggttctgtgc cgctgttgcg gatcatctcc agcgtattac gcgacgtgcc
     3241 gcaggccggg ttgtgataaa tggtaatgtt gctcatatca gtatctcatt acaaagtgaa
     3301 agagagacgt agcgccagcg cagccagcgt cacaaacagc acaggcaggg tcatgataat
     3361 ccctgtacgg aagtaatatc cccagctgat agtcatattc ttctgcgaaa gtacgtgcag
     3421 ccagagtagc gtagccaggc taccaattgg ggtaattttc ggtcccaaat cgcagccaat
     3481 cacattggca taaaccatcg cttctttgat aacgccagat gccgtgctgc catcaatgga
     3541 caacgcgcca accagtaccg tcggcatatt gttcataata gaagagagga aggcggtgag
     3601 gaatccggtg ccgagcgtcg cggcccacag gccgttatcc gccagcacgt tgagtacgcc
     3661 agaaagatat tccgttaatc cggcattgcg caggccataa accaccagat acatgccgag
     3721 cgagaagatg acaatctgcc agggggcacc gcgcaggact ttacccgtat taatcgcatg
     3781 accgcgttta gcgacgacaa ataatatcag cgcgcccaca gctgcaatgg cgctcaccgg
     3841 aatgccgagc ggttccagga cgaaaaatcc caccagcaga agcagtaaaa caacccagcc
     3901 agttttgaac gtagcaggat ctttgatcgc ttctgcggga gatttcagca gcgccatatc
     3961 gtagttctgc ggaatatctt tgcgaaaata gagatgtaac atcaccagcg tggcaacaat
     4021 cgcggcgata tccaccggca ccatcaccga ggcgtattcg cgaaagccga ggccaaagaa
     4081 atcagcggaa acgatattca ccaggttgga gacaataagc ggcaggctgg cggtatcggc
     4141 aatgaatccg gccgccatca cgaacgccag cgtagtgcct ttactgaacc ctaaagccag
     4201 cagcatggcg atgacaatcg gtgtcaaaat aagcgccgcg ccatcattgg caaacagggc
     4261 ggcaacggca gcaccgagca ggacaatcca ggtaaacagc aagcgaccac gaccattacc
     4321 ccagcgtgag acgtgcagcg ccgcccattc aaaaaagccg gactcatcca gcagcaggct
     4381 gatgataatg acggcgataa acgcagccgt cgcgttccag acgatattcc acaccaccgg
     4441 aatatcaccc ggatggacca cgcccgtaac taacgccagt actgcgccga gcgttgcact
     4501 ccagccgatg cctaaacctt tcggctgcca gataaccaat acgatggtca ggacaaagat
     4561 agcgcctgcc agtaacataa tgcctcccgg ataaaacaca tctgaaaatt catatgtgtt
     4621 tagctaaatt tttaactgca aatgttctta ctgtccccgg aacagttttg tcgagccagg
     4681 ttgcggacaa tcgcctgaac cttttcctgt tcacatcgcc aggcctcatc aataattttc
     4741 gccgcccatg ctggaatatg cggtgataag cggtaatgaa cccacttacc ttgcttgcgg
     4801 tccagcaata gcccgctttc acgcagcaat gccaggtggc gggagatctt gggctgcgac
     4861 tggtcgagag cagtgcagag atcgcagacg cataactctc ccagttcgct gagcagtaaa
     4921 acgatgccca gacgggtttc atcagcaaga attttgaaca attggatggg taacagaaat
     4981 gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga taagtcaaaa
     5041 acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg aattttgagt
     5101 tggctattca tttgaaagga ggtctgaatc aggacgtaaa aaaaccgact ttgcgtcggt
     5161 tttttacttt ccagccctga gttggtggct ctggctggag tgagagagtt cttatctaac
     5221 agctcaatac tattagccag tggctaacat tgcaagaatt agcgatttct tcagctggcg
     5281 gagggggcac ttcggtatat atcgcaatga cgatttatat actttcactg ggtcatcgtc
     5341 atattaagcc ttgtccgaat ccggatgtaa aaaaaccgac tttgcgtcgg ttttttactt
     5401 tccagccctg agttggtggc actggctgga gtgagaatgc cattatctaa cgcatcaatg
     5461 ctaataccat actgaaacta ttgcaaggac atgctggttt tataacctga atgtactgta
     5521 tgattatcca gttagctctg aggcattttc actctggtaa tgcgcataaa cgctttcaaa
     5581 gtcctggtca gaagtacggg tggtgccgtt aactgatgct ctggccggag tgagaatgcc
     5641 attatctaac aatagggcat gcgccgtgac aggcagtgga tgagtaagcg gatgcattct
     5701 cactccatcg catggagaaa acgggtgatt gataaagcaa tcatcgttct tggggcgtta
     5761 attgcgctgc tggaactgat ccgctttctg cttcagcttc tgaactgata gcggaaacgt
     5821 aattaagggc taagagcaca ctactcttag ccctttaaca tttaacgcat tgtcacgaac
     5881 tcttctgccg ccgttgggtg aatggcgacg gtattgtcga agtctttttt ggttgcaccc
     5941 atcttcagcg ccaccgcgaa gccctgcaac atttcgtcca taccaaagcc aatgccgtga
     6001 ataccgacaa tcttctcttc cgatccaacg cacaccagct tcatgcggca cggctggcgg
     6061 tgagtggtga cggcggtata catcgcggtg aaagaggatt tatacacttt cacctgatcg
     6121 tcgccatact gctcgcgcgc ctgcggttcc gttaaaccaa cagtaccaat cggcggatgg
     6181 ctgaagacca cggtcggaat gttgctgtaa tccagatgct catccggctt gttattaaac
     6241 aggcgttcag agagacggcg acccgctgca actgccaccg gtgtcagctc cactgcaccc
     6301 gtgttatcgc ccaccgcgta aataccttca atattggtgt tttgatattt atcgacgacg
     6361 atatagcctt tttcgttagt tttaacgcca gcggcttcca ggttgatgtt gtcattggca
     6421 ggctcgcgac caatcgccca aatcaggcaa tccaccgttt cactgcgacc atcttccagc
     6481 tccagcgtca ggctaccatc ggcatttttc actaccgctt tcgggatggc gttggtgtgc
     6541 agctgcgggc cttcggcgtt catcacttcg accagcgttt cggaaatcat cgggtcgaag
     6601 ctgcgcagcg gcgcatgttt acgcacaaac agatgcgttt tcgcgccgag gccgttaatc
     6661 acgcccgcca gctcaacggc gatgtaaccc gcgccaacaa ccgccacgcg ctctggcaaa
     6721 gcaggaaggg cgaagaagcc atcagaatca ataccgtatt ccacgcccgg aatatccggg
     6781 tggctcggac gaccgcctgt ggcgatcaga atatgatcgg ccgtgatggt ttcgccgttt
     6841 acctccagcg ttttggcatc aacgaagcgg gcaaagcctt tgattacatc aacgttattt
     6901 ttaccgagca cgttttcata ggaagtatga atacggtcga tataggcggt acggctggcg
     6961 atcaacgttt cccagttgaa tttattgata gtggtatcaa aaccataatc cgggccgtac
     7021 atatggatcg cttcacggat ttgcgccgcg tgccacatca cttttttcgg cacacagcca
     7081 acatttacgc aggtgccgcc cagctctttg gcttcaatca gcgcacattt ctggccgtac
     7141 atagccgcgc ggttgatgga ggcgataccg ccgctgccgc cgccgatggc gatgtaatca
     7201 tagtgtttag tcatgacaaa gtgtccttat cgttgattac cgcgattgta gcgcgagacg
     7261 taataggtgc cagcaatggc tgcaattact ccggcacgat ccagcttacg gtggcgtgcc
     7321 cggtgcctgc cggaaccagt ttgctgtgca gccacggcag cacgttattc atctgctgtt
     7381 ccagtttcca cggcgggtta atcacaatca tgccggaagc ggtcatgcca cggcgatcgc
     7441 tgtctggcag taccgccagt tcaatttgca gaattttgcg aataccggtc gcttccagat
     7501 cgtggatcat gcgcttaatt tgctgacgca gcaccaccgg ataccacagt gcgtaagtac
     7561 cagtggcgaa acgtttgtaa ccttctgcta tcccgctgac caccgcctga tagtcggttt
     7621 tcatttcata cggcgggtcg ataaggatta aaccacggcg ggaaaccggc ggcagtttgg
     7681 ccttaagctg ctggaaacca tcggcttttt cgacgcgcgc acggctgtct ttctgaaatt
     7741 cagaacgcag caacgggtaa tcgctcgggt gcagttcggt cagttgcagg ctgtcttgtt
     7801 cgcgcagtag ctggcgagca atcaatggcg aaccggggta gtaacgcaac tgaccgctac
     7861 ggttgaagtg ttttaccaca ttgatgtacg cctccagttc tgcgggcaaa tcgtcctgct
     7921 gccagatacg ggcgatgcct tcgagatatt cgccggtacg ctcggcatgt tcgctgccta
     7981 actgataacg cccggcccct gcgtgggtgt cgagatagag aaacggttta tctttctctt
     8041 tcagcgactc gatgatcagg ctctgaacgg tatgtttaag gacgtcggcg tggttgccag
     8101 cgtgaaagct gtggcgataa ctgagcatgg gtaaaggtgt tccggtaagt aaaaatcggc
     8161 ccgtattcgg ggcgcacaaa agcgtatcag gacagtatac cgaaaagagg ccgccgccgc
     8221 gaaagcgtaa cgtttctcat tgaaattcac tacacttaac cccatgctac acacattatg
     8281 taaagcgcct gttgagcgct tccttaacct ctttaaccag gactgcgcga atgacgaatc
     8341 cgttactgac tccctttgaa ttgcctccgt tttctaaaat tctcccggaa catgtcgttc
     8401 cagccgtgac taaggcgctg aacgactgcc gcgaaaatgt ggagcgcgta gtagcgcaag
     8461 gagcaccgta cacctgggaa aatctctgcc agccgttggc ggaagtggac gatgtgctgg
     8521 ggcgtatctt ctccccggtc agccacctga actcggtgaa aaatagcccg gaactgcgtg
     8581 aagcgtacga acaaaccctg ccgctgctgt cggaatacag cacctgggta gggcaacatg
     8641 aagggctgta taaagcgtat cgcgacctgc gcgatggcga tcattacgcc acgctgaaca
     8701 cggctcagaa aaaagcggtt gataacgcac tgcgcgactt cgaactctct ggcatcggtc
     8761 tgccaataga gaaacaacaa cgctacggtg agattgccac tcgtctttcc gagctgggca
     8821 accagtacag caacaacgtc ctcgatgcga cgatgggctg gaccaaactc gttaccgacg
     8881 aagcggagct ggcggggatg cctgaaagcg cgctggctgc ggcaaaagcc caggccgaag
     8941 cgaaagagct ggaaggctac ctgctgacgc tggatatccc aagctacctg ccggtaatga
     9001 cctactgcga caaccaggcc ttgcgtgaag agatgtatcg cgcttacagc acccgcgcct
     9061 ccgatcaagg cccgaacgcc ggtaaatggg ataacagcaa ggtgatggaa gagatcctcg
     9121 ctctgcgtca cgaactggcg caactgctgg gctttgaaaa ctacgccttt aaatcccttg
     9181 ccactaaaat ggcagaaaac ccgcagcagg tgctggattt cttaaccgat ctggcaaaac
     9241 gcgcgcgtcc gcaaggcgaa aaagagctgg cacaactgcg cgccttcgcc aaagcggaat
     9301 ttggcgtcga tgagttgcag ccgtgggata tcgcgtacta cagcgaaaaa caaaaacagc
     9361 acctctacag catcagcgac gagcagctgc gtccgtactt cccagaaaac aaagcggtta
     9421 acggcctgtt tgaagtggtg aaacgtattt acggcatcac cgctaaagag cgtaaagatg
     9481 ttgatgtctg gcatccggat gtacgtttct tcgaactgta tgacgaaaat aacgaactgc
     9541 gcggcagctt ctacctcgat ctgtatgccc gtgaaaacaa gcgcggcggg gcgtggatgg
     9601 atgactgcgt aggccagatg cgtaaagctg atggttcgct gcaaaaaccg gtcgcgtatt
     9661 tgacttgtaa cttcaaccgc ccggtaaatg gtaaaccggc gctgttcact cacgaggaag
     9721 tgatcaccct gttccacgag ttcggtcacg gcctgcacca tatgctgacc cgcatcgaaa
     9781 ccgctggtgt ttccggtatc agcggtgtgc cgtgggatgc ggtcgaactg ccgagtcagt
     9841 ttatggaaaa ctggtgctgg gagccggagg cgctggcgtt tatctccggt cactatgaaa
     9901 ccggcgaacc gctgccgaaa gagttgctgg ataaaatgct ggcggcgaag aactaccagg
     9961 cggcgctgtt tattctgcgt cagctggagt tcggcctgtt tgatttccgc cttcatgccg
    10021 agttccgccc ggatcagggg gcaaaaatcc tcgaaactct ggcagaaatc aagaaactgg
    10081 ttgccgtggt gccatctccg tcctggggcc gtttcccgca cgctttcagc catattttcg
    10141 ccggtggtta tgcggcaggt tactacagct acctgtgggc tgacgtactg gcggcagatg
    10201 ctttctcgcg ctttgaggaa gagggcattt tcaaccgtga aaccgggcag tcgttcctcg
    10261 acaacattct gagccgtggc ggttcagaag agccgatgga tctgttcaaa cgcttccgtg
    10321 gtcgtgaacc gcagctggat gcgatgctgg agcattacgg cattaagggc tgatcattca
    10381 gtgaaaatct gcttaattga tgaaacaggc gccggagacg gcgccttatc tgttctggcg
    10441 gcccgctggg ggctggagca cgatgaagac aacctgatgg cgctggtgtt aacgccggaa
    10501 catctggaat tgcgcaagcg tgatgagcca aaacttggcg gcatatttgt tgattttgtt
    10561 ggcggagcga tggcgcaccg acgcaaattc ggcggtggtc gcggtgaggc ggtggcgaaa
    10621 gcggtgggca ttaaaggcga ttatttgccg gatgtggtgg acgccaccgc agggctgggg
    10681 cgcgatgcct ttgtgctggc ttcagtcggt tgccgcgtgc ggatgctgga gcgtaatcca
    10741 gtcgttgccg cattgctcga cgacggcctg gcgcgtggtt atgcggatgc ggaaatcggc
    10801 ggctggttgc aggagcgttt gcagttaatt cacgcctcca gcctgacggc gctaaccgat
    10861 attaccccac gcccgcaggt ggtctatctc gacccgatgt tcccgcataa gcagaaaagc
    10921 gcgctggtga aaaaagagat gcgtgtcttt cagtcgctgg tggggccgga tcttgatgcc
    10981 gatggattgc tggagcctgc acgcttgctg gcgaccaaac gcgtggtagt aaagcgcccg
    11041 gactacgcgc caccgctagc gaatgtcgcc acgccaaacg cggttgtgac taaagggcat
    11101 cgctttgata tctatgcagg tacgcccgtg taaaagtaaa gcagggtagc gataacgcta
    11161 ccctgctttc tgcaagaata attaatggct ttccggcgtc gcaatcatgc gtttcagcca
    11221 cggcaccatc aacagcatca ctactgcaac gcccagcgtg accagaccaa tcttaccaaa
    11281 cacgttggta tagacgggca acgtctcaag cggatcggta atgttgtccg gcaccgcggt
    11341 aaatgttgcc acatagccgc ccagcaagaa cgcggcagcc tgcgtcagga accacatccc
    11401 gagaataaag cccatcaaat gctgcggcac cagggcagca atcatcgcca ggccaagggc
    11461 gctaataaac agttcaccta agctctggaa taagtacacc agcacgataa accatggcga
    11521 tgtcagccct tgtgcatccg caaaccacat tcccgcagct gccgccgtca aaaagcccag
    11581 tgagcacata aacatgccga gagtaaattt catcggcatc gagaggtctt tgcctttgtt
    11641 acccagatgc gtgtaaatgc ctgccagtat tgggctggcg agtaccaccc agaacgggtt
    11701 aagcgcctgg aagctgaccg ggttgatgga aaaaccgaga atttcatgat gcacgttgtt
    11761 gatggcaaag aagttcagcg atgttggcat ctgggcgtag agaatgtaaa acaccaccgc
    11821 ttcgagcatc aggacaaagg cgacaaacat tttattgcgc ccggttttat ccagcttgaa
    11881 tgcctgacga aagaagatga tggtgacgac gatggagaga acaatcagca ccagattggc
    11941 gacttctacg ttgtgcatca gccatgcaca tacgaagatc atcaccacgc tgccaagtaa
    12001 tacgtacaac agtttgctga agctcatcgg cttgaagtcg ggttcagaac caatgtcttt
    12061 caccattcca cgacaggcga tgtaaaccag taatgcgata attaaccccg ccccgcacag
    12121 gttgtaggtg actgaataac cgaatctatc agcgatcaca ggggccagcg ataacgctat
    12181 caacgagccg atattgatcg acatatagaa cagggtgaat gcgccatcaa gccgcggatc
    12241 tttcggcgga tagcacttcg aaagcaagct ggctgggtta gctttaaaca ggccgttacc
    12301 gacagcgata gtccccaggg cgatgaaaat caggtcaggc ttaagtagcg acatgccggt
    12361 catgaagtag ccaatcgcca gcacaagtgc tccgagaaca atggtgcgtt tggtccccag
    12421 caggtggtcg ccgacatagc cgccaatgga aatgaggcca tagaccagcg cagcaaaagc
    12481 accaaaagtg acaaaagcct gctcttgcga gaatccaagc tgtttaacga agaaaaccgc
    12541 cagtacgccc tgcacgccgt agtagccgaa tcgctcccat aactcgacaa aaaagatcat
    12601 gaaaaatggg cgaggttgct gcagcatccc catgggtgtt gttgtattca tatttattaa
    12661 ccttccaata ccatcctgaa aagtatgaac gcgtaaggga taacgcgcga aaaagtttac
    12721 atctgtcaga gattcttaat attgatgcgg tgaatagatt aatacactat tgcaacagaa
    12781 aataaccagc tgatcacact aatgatggaa aatatgatgg tgttaaatgt tgtattggca
    12841 ggagattttg attgcgtacg cagatcgtag gccagataag gggtttacgc tgcatcaggt
    12901 aacaaggcgc gccaaaaagc aaaacgcccg catatgcggg cgttcaggaa cacgtcgtgg
    12961 agggatgatt attcttcttc gtcgcgcagc ggaacaatca gcatatcaac gtgaacggtg
    13021 ttgatcagct gacgtgcgga agacatcagt ttgctccaga agtcctggtg gtgaccacaa
    13081 accaccagat ccatatcgta tttcttgatt gcatcgacca gaacctggcc caggtcgccg
    13141 ctgccgctca gggtttcagt gattgggtag cctgcattag tggaaagctc ggtcagcgca
    13201 tgatgtgtct cttcagagat gcgtttctgc atatcaccca gattcacatc aataagcccg
    13261 gtgtataggt cagagtagtt tacatctacg tggatcagag aaactttcgc attgtagggg
    13321 cgagccatag agactgcttt ctctaccaga actttgcttt ccggggagag atcgactgcg
    13381 atgagaatgt gtttataagc catagtgtta ctccttccat aaagttgtcg atgactggcc
    13441 agctagcgtt tcttgtgccg ttacgtcacc cgcgtcctgc gaactacgtg cgcggggcta
    13501 acccagccgt aaagattcag tgttaagacc atccttacct tatagcgacc cggatgatcc
    13561 gtcaatccgc cttgcttacc cgttagataa acaaattttc gacatgaata tattgatagt
    13621 ggttaacctt ctggaaaaaa aacaacctga tctcctacac tatctataga gccgctcgga
    13681 tgttggtcac cacaaaagcg gtttctccag gacctggtgt taactgattg gcagcgtatc
    13741 gggagcggta gccccggggt ggaactccgc gggcaggtcg ccggggagga ggtatgataa
    13801 gcaccgtcgc attattttgg gctttatgtg tcgtttgcat tgttaacatg gcgcgctatt
    13861 tctcatcact acgcgcgttg ttagtggtac tgcgtaactg cgatccattg ctctatcaat
    13921 atgttgatgg agggggcttt tttacctcac atggccaacc caacaaacag gtgcgtctcg
    13981 tttggtatat ctatgcccaa cgttatcgcg atcatcacga tgatgaattt attcgccgct
    14041 gtgagcgggt gcgtcggcag tttattctga ccagcgcatt gtgtggtctg gtggtggtca
    14101 gcctgattgc attgatgatt tggcattaag cacttcagca ataataacgc ataaaaaaag
    14161 cgggccagta aactgacccg ctcgagactg attacaaacg caaaattgcc tgatgcgcta
    14221 cgcttatcag gatctacata atctctgcaa tctattgaat ttgcatgttt ttgtaggccg
    14281 gataaggcgt tcacgccgca tccggcatga acaaagcgca ctatgtcaca atctgaagcg
    14341 ggccagctga cccgctcgtt ttggtgtgta cgattacagg aactgcaagg agagccagta
    14401 cagtccgccg gaaagcagta ccgcagccgg aagggtaaac acccaggcca tcagaatact
    14461 ggttacggtt ttacgctgta agccgccgcc atctaccacc atcgtccccg cgacagaaga
    14521 ggagagtacg tgagtagtgg aaaccggcat cccggtataa ctcgccaggc cgatagacac
    14581 tgccgccgtc atctgggccg acatcccctg agcgtaggtc atgcctttct taccgatttt
    14641 ctcaccgata gtcgttgcca cacggcgcca gccaatcatc gtaccgatac ctaacgccag
    14701 cgcgaccgcc atgatgatcc acaccggtgc atactcgatg gtgctaagca tgtcggactt
    14761 cagtttcttc aacaggcgct gatcgtcagc actcacacca ggcatcttca ccactttgtc
    14821 gatagtgtca gaaacgcaca gcataatgcg gcgcatctgg ctacgttgat caagcgacag
    14881 cttgtcgtag ctttccacat cggtagtcaa catgcctttc aggcggttga gcgcgttaat
    14941 ggtattcgac ggatggcaat ggaactccgc gggttgcgtt gcgccagctt ccggagccgg
    15001 tactaactga tcagcaccgg tagcctgttt gagcagcgca ggatgctgct caaagtaagc
    15061 ttcgacgttg ttgatggcat cacgggtacg ggtgatttcg tagccagtgg cattcatgtt
    15121 caccacgaag cctgctggtg cgacgccaat caataccaac ataaccagac caatgccttt
    15181 ctgaccatcg ttcgcgccgt gcgaaaacgc cacgccgata gcggaaagga tcagcgcaat
    15241 acgcgtccag aacggcggct ttttcttgcc gtctttcttt tcacgctccg ctggggtcag
    15301 gtggatacgg gcgcgtttct tggtgccgct ccagtagcga cgcagcaaga aaatcagacc
    15361 gccagcaaac accaggccga caatagggga aacgatcaga gaaccgaaaa tacttaatac
    15421 tttcgggata ttgagtgcat ccaccactga cgtcccggtc atcaacgcat tggttaaacc
    15481 aatcccgatg atcgcgccaa tcagcgtatg agagctggat gcaggtaaac caaagtacca
    15541 ggtacccagg ttccagataa tcgccgccag caacatagag aacaccatgg caaggccatg
    15601 agacgatccc atattaagca gcagatccgt cggcagcata tgcacaatgg cataggcaac
    15661 actcagacca cccagcaaaa cacccaaaaa gttgaacacc gctgccataa ccacggcgag
    15721 ctgagaacgc atcgcgcggg tatagataac ggttgccacg gcgttggctg tgtcatggaa
    15781 accattgatg gcttcgtaga acagcacaaa agccagtgca agcaataata acagcccggt
    15841 atgcaaatcc aggccagcaa acaaatgtag cataggacgt tacgccattt tgaggacatg
    15901 aacgcggcgc attatcagtg actttcgcgg cgcgggcaaa gtgaaatata gacttttttt
    15961 gatttgcctc ctgtatggat ttcactcaaa aaataattat cttatataat tcaggcaaat
    16021 acttcctttt agtaatattg atgctggtgc gaccactgag gaatctttac aattcacgcc
    16081 cgttttttct aagaggagcg caacgtggaa aggtttgatg ccattattat aggcgctggt
    16141 gcggcgggta tgttctgttc tgcgctggca ggtcaggcag gacgccgggt tctgctgatc
    16201 gataatggta aaaaaccagg gcgcaaaatc cttatgtctg gcggtgggcg ctgcaacttt
    16261 accaaccttt atgtcgaacc aggcgcttat ctgagccaga atccgcattt ttgtaagtct
    16321 gcactcgcgc gttttaccca gtgggatttc attgatctgg tcaataaaca cggcatcgcc
    16381 tggcacgaga aaacgttagg acaactcttc tgcgatgact ccgcgcagca gattgtcgac
    16441 atgctggtgg atgagtgcga gaagggcaat gtaaccttca gattgcgtag cgaagtgctg
    16501 agtgtggcga aggatgaaac aggcttcacg cttgaactga acggcatgac tgtcggttgc
    16561 gaaaagctgg tcatcgcgac cggtgggctg tcaatgccgg ggctgggcgc gtcgccgttt
    16621 ggttataaga ttgccgaaca atttggcctc aacgtgctgc cgacccgcgc gggtctggtg
    16681 ccattcactc tgcataaacc gttgctcgaa gagttacagg tgctggcggg cgtggcggtg
    16741 ccttccgtga ttaccgctga aaacggcatc gttttccgtg agaacttact cttcacccat
    16801 cgcggcttgt ctggaccggc ggtgttgcag atttccagct actggcaacc gggtgaattt
    16861 gtcagtatca atctgctacc ggatgtggat ctcgaaacct tcctgaatga gcagcgtaac
    16921 gcacatccga atcagatcct gaaaaacaca ctggcggttc atctaccgaa gcggttggtt
    16981 gaacgcttac agcaactcgg gcaaatcccg gatgtttcgc taaaacagct caacgtgcgt
    17041 gaccaacagg cactgattag cacattgacc gactggcgcg tacaacccaa cggcactgaa
    17101 ggctatcgca ctgccgaagt gacgctcggc ggcgtggaca ccaacgaact ctcttcacgg
    17161 acgatggaag cgcgcaaagt gcctgggctg tacttcatcg gcgaagtgat ggacgttacc
    17221 ggctggctgg ggggctataa cttccagtgg gcgtggtcga gtgcgtgggc ttgtgcgcag
    17281 gatttgattg cagcgaagtc gtcctgacct gttacctaaa gatgaaaatt attagagccg
    17341 cattaaaaat gagctaattt tgatagtggc tatcttgtga ttattttcta atgagcccgt
    17401 gaactgaaac cctccaggct taatataagg tggaaggaaa ggtgattgaa aactcaccca
    17461 gtggccagat ctttataaaa tatgaacagg ttgaaaaaaa cagtaacttt gttgtttttt
    17521 tatccttaac cactatgcat taatgctgcg ttatttcatg atgcctaaga aaaaccagcg
    17581 ttacgcaaat ggtcaacgct ggttttatcc ggtacgttgc aattattttt tagcagaacc
    17641 tgcttctaat attgaaacga ttgagaacaa cgtaaagcat accgctccta aaccgaccaa
    17701 aacccgcgaa ggaataaaat atcccatgtc ggtctgcgcc atttccgcaa gaaacgatgc
    17761 caggaaaagg caaaacaggc aggtgaagac gggaatcatc ggtattctgt tggctaacga
    17821 acaggtacgt ctccatacca gtgccagtag ccagactttt gagaatatgc tgtaacagat
    17881 cattccgagg caaataagaa taatgccggg agccagtcgg gcgcttgcat cggaactgac
    17941 taagacgtat atcccctgca gtaccgtgat tgagccgaga aaaataaccc aataacacca
    18001 cagccagtgt tctttagttg aaaacgtatt acgtgtttga tggacaattg tggcaacaag
    18061 gccaattaga caggcacaga ttgcggttaa cccaagcaat acgtgacccg cgacataatg
    18121 cggagttatg tcagcactac gtaacagcgt aatagaccaa atgaaaccga gcagggtgag
    18181 taaaacggga acggcaatga ggcaattacc gattaatgaa gaataagcct gtaccggcgt
    18241 tccatcgctc ttgctccccg ctgcattttt gggaatgagc agaaagtgac cggatgacgc
    18301 tgccaccgtc gatacacagg cggcaatcat accaacgccg aaaataacat ggccggcgac
    18361 aaactcgtct gccatcacat cattgcctgc taacagtgcc caaccccata tcatggtgat
    18421 aattgacccc gcatagccaa taatggggaa caatgtattg taaaacgtat taacgccgcg
    18481 cgtgagctgc gaaatgataa taaatgcggt agtgaataat gccaggcata tggctgccag
    18541 agaaatcagc acatgacccg caacgaaata ctcactttgc ccagaacttt ccagcacaaa
    18601 gccacctaac gcgatacaaa ttaagcccat aattaaaggg attaatttga aaagccaccc
    18661 gatatatatg ttcactttaa actcctcagg tatccctgct aatattgaaa tgtgcattaa
    18721 tgataatttc agcctgttaa tataatgacg agatatcatt aaagacaaat gcatagcata
    18781 actatagcgg gatttacttt gcagagcgct acttttcagc gttcaaaagg ccatggtaaa
    18841 taaatattta tcctaagatt aattcttatt ctgttgtttt ttacaattat cttccaggga
    18901 gggattcttt ttcattattt ggtaaaagac cggatggtat cggtgcttaa aatcagcgaa
    18961 catataagta ttattgtttt atatcgtcga aaatatgtct ttcagacatg tattatttat
    19021 tttgttacta acaagtcaaa aatatcgcag gttatttatg aaagcaattg acaatatatt
    19081 taagtacgtc ccttcaaacc ataaagataa gcattcagat aaagttaata atcatcaaca
    19141 ccatagcaag gttgataaaa cacatcgttc aaagatagtt gaaattgata aactggataa
    19201 tgattcgcaa atagataacg attttggcat gcatatcatc tatttcctgc aacatggtca
    19261 ctggagggta aatgatcgta gtcatcagat ggagaaagta tggttttaca atagtgaacc
    19321 ttctatagat attcaa
//
LOCUS       DAZGJW010000085        19105 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZGJW010000085 DAZGJW010000000
VERSION     DAZGJW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.085"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(225..872)
                     /gene="ychE"
                     /locus_tag="ACHKIK_003057"
     CDS             complement(225..872)
                     /gene="ychE"
                     /locus_tag="ACHKIK_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO9084183.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(903..956)
                     /gene="ychE"
                     /locus_tag="ACHKIK_003058"
     CDS             complement(903..956)
                     /gene="ychE"
                     /locus_tag="ACHKIK_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO9084184.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1349..4024
                     /gene="adhE"
                     /locus_tag="ACHKIK_003059"
                     /gene_synonym="adhC"
     CDS             1349..4024
                     /gene="adhE"
                     /locus_tag="ACHKIK_003059"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO9084185.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4148..5044
                     /locus_tag="ACHKIK_003060"
                     /pseudo
     CDS             4148..5044
                     /locus_tag="ACHKIK_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5326..5943)
                     /gene="tdk"
                     /locus_tag="ACHKIK_003061"
     CDS             complement(5326..5943)
                     /gene="tdk"
                     /locus_tag="ACHKIK_003061"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO9084186.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            6548..6961
                     /gene="hns"
                     /locus_tag="ACHKIK_003062"
     CDS             6548..6961
                     /gene="hns"
                     /locus_tag="ACHKIK_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO9084187.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLASVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(7105..8013)
                     /gene="galU"
                     /locus_tag="ACHKIK_003063"
     CDS             complement(7105..8013)
                     /gene="galU"
                     /locus_tag="ACHKIK_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO9084188.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8215..9228)
                     /gene="rssB"
                     /locus_tag="ACHKIK_003064"
     CDS             complement(8215..9228)
                     /gene="rssB"
                     /locus_tag="ACHKIK_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO9084189.1"
                     /translation="MTQPLVGKQILIVEDEPVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9320..10225)
                     /gene="rssA"
                     /locus_tag="ACHKIK_003065"
     CDS             complement(9320..10225)
                     /gene="rssA"
                     /locus_tag="ACHKIK_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO9084190.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            10338..10796
                     /gene="ychJ"
                     /locus_tag="ACHKIK_003066"
     CDS             10338..10796
                     /gene="ychJ"
                     /locus_tag="ACHKIK_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO9084191.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10846..11688
                     /gene="purU"
                     /locus_tag="ACHKIK_003067"
     CDS             10846..11688
                     /gene="purU"
                     /locus_tag="ACHKIK_003067"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO9084192.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     SDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            11848..11932
                     /locus_tag="ACHKIK_003068"
     tRNA            11848..11932
                     /locus_tag="ACHKIK_003068"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11882..11884,aa:Tyr,seq:gta)
     gene            11980..12111
                     /locus_tag="ACHKIK_003069"
     ncRNA           11980..12111
                     /ncRNA_class="other"
                     /locus_tag="ACHKIK_003069"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(12295..12972)
                     /gene="narI"
                     /locus_tag="ACHKIK_003070"
     CDS             complement(12295..12972)
                     /gene="narI"
                     /locus_tag="ACHKIK_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO9084193.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12972..13682)
                     /gene="narJ"
                     /locus_tag="ACHKIK_003071"
     CDS             complement(12972..13682)
                     /gene="narJ"
                     /locus_tag="ACHKIK_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO9084194.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13679..15217)
                     /gene="narH"
                     /locus_tag="ACHKIK_003072"
     CDS             complement(13679..15217)
                     /gene="narH"
                     /locus_tag="ACHKIK_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO9084195.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(15214..18957)
                     /gene="narG"
                     /locus_tag="ACHKIK_003073"
     CDS             complement(15214..18957)
                     /gene="narG"
                     /locus_tag="ACHKIK_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO9084196.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         4823 a         5004 c         4574 g         4704 t
ORIGIN      
        1 aattactaat atcatttcaa ttattcttca gcaagcccag gattatgatg tggcgaaaat
       61 aacatgatgg tgaggagaag cagttaagga aatccttata actaactgta atttgagaag
      121 aatattttta cctattcctt ttgcttttga taactgcgcg ggaacaaagt aaaaataatg
      181 acgaagtaaa cggagcatgg cagctccgtt tcatttgaaa ggaattaatt aagcaggccg
      241 gggaaaatcc ccttaatacc agtaacgata aattcaatcc ccaatgccat cagcaatagc
      301 cccataatac gcgtaatcac gttgatgccg gtctggcgta aaacccgtac cagccacggt
      361 gccatgcgga acaatcccca gcaacataaa gcgaacaatg caatagccac aaagaaacca
      421 aacagatagc taatgctgtg ataacgcgta ccccagacga tggtagaact gatcgcccct
      481 ggccccgcca tcaacggcaa cgccaatggc accacaccaa tgctttcacg taccgcggtt
      541 tctgattttt cttgcttgtt ctgtttatcc tcgccaagct tgccgctgat catcgacatc
      601 gctatcgtca ccaccaggat gcccccggcg atacggaacg aatcaattga tataccaaaa
      661 agttgcagaa tcgtgtcgcc gagaaaaagc gagatccaca agataatggc cacagacagg
      721 ttggctgtaa ggttagtttt gtttcgcgct gccgctgtct gataactggt catgctgata
      781 aagacgggaa taatccctac cgggttgacc agcgcaaata acccgatgaa aaatttgaag
      841 taaacgggaa aatcaaaaaa ggtctgaatc acggttagct ccgaagcaaa agccggataa
      901 tgttagccat aaataaggtt gaaaagacgc gctgacaata cgccttttga cagcattttt
      961 cacctcctaa ctacttaaaa ttgctatcat tcgttattgt tatctagttg tgcaaaacat
     1021 gctaatgtag ccaccaaatc atactacaat ttattaactg ttagctataa tggcgaaaag
     1081 cgatgctgaa aggtgtcagc tttgcaaaaa tttgatttgg atcacgtaat cggtacccag
     1141 aagtgagtaa tcttgcttac gccacctgga agtgacgcat tagagataat aactctaatg
     1201 tttaaactct tttagtaaat cacagtgagt gtgagcgcga gtaagctttt gattttcata
     1261 ggttaagcaa atcatcaccg cactgactat actctcgtat tcgagcagat gatttactaa
     1321 aaaagtttaa cattatcagg agagcattat ggctgttact aatgtcgctg aacttaacgc
     1381 actcgtagag cgtgtaaaaa aagcccagcg tgaatatgcc agtttcactc aagagcaagt
     1441 agacaaaatc ttccgcgccg ccgctctggc tgctgcagat gctcgaatcc cactcgcgaa
     1501 aatggccgtt gccgaatccg gcatgggtat cgtcgaagat aaagtgatca aaaaccactt
     1561 tgcttctgaa tatatctaca acgcctataa agatgaaaaa acctgtggtg ttctgtctga
     1621 agacgacact tttggtacca tcactatcgc tgaaccaatc ggtattattt gcggtatcgt
     1681 tccgaccact aacccgactt caactgctat cttcaaatcg ctgatcagtc tgaagacccg
     1741 taacgccatt atcttctccc cgcacccgcg tgcaaaagat gccaccaaca aagcggctga
     1801 tatcgttctg caggctgcta tcgctgccgg tgctccgaaa gatctgatcg gctggatcga
     1861 tcaaccttct gttgaactgt ctaacgcact gatgcaccac ccagacatca acctgatcct
     1921 cgcgactggt ggtccgggca tggttaaagc cgcatacagc tccggtaaac cagctatcgg
     1981 tgtaggcgcg ggcaacactc cagttgttat cgatgaaact gctgatatca aacgtgcagt
     2041 tgcatctgta ctgatgtcca aaaccttcga caacggcgta atctgtgctt ctgaacagtc
     2101 tgttgttgtt gttgactctg tttatgacgc tgtacgtgaa cgttttgcaa cccacggcgg
     2161 ctatctgttg cagggtaaag agctgaaagc tgttcaggat gttatcctga aaaacggtgc
     2221 gctgaacgcg gctatcgttg gtcagccagc ctataaaatt gctgaactgg caggcttctc
     2281 tgtaccagaa aacaccaaga ttctgatcgg tgaagtgacc gttgttgatg aaagcgaacc
     2341 gttcgcacat gaaaaactgt ccccgactct ggcaatgtac cgcgctaaag atttcgaaga
     2401 cgcggtagaa aaagcagaga aactggttgc tatgggcggt atcggtcata cctcttgcct
     2461 gtacactgac caggataacc aaccggctcg cgtttcttac ttcggtcaga aaatgaaaac
     2521 ggctcgtatc ctgattaaca ccccagcgtc tcagggtggt atcggtgacc tgtataactt
     2581 caaactcgca ccttccctga ctctgggttg tggttcttgg ggtggtaact ccatctctga
     2641 aaacgttggt ccgaaacacc tgatcaacaa gaaaaccgtt gctaagcgag ctgaaaacat
     2701 gttgtggcac aaacttccga aatctatcta cttccgccgt ggctccctgc caatcgcgct
     2761 ggatgaagtg attactgatg gccacaaacg tgcgctcatc gtgactgacc gcttcctgtt
     2821 caacaatggt tatgctgatc agatcacttc cgtactgaaa gcagcaggcg ttgaaactga
     2881 agtcttcttc gaagtagaag cggacccgac cctgagcatc gttcgtaaag gtgcagaact
     2941 ggcaaactcc ttcaaaccag acgtgattat cgcgctgggt ggtggttccc cgatggacgc
     3001 cgcgaagatc atgtgggtta tgtacgaaca tccggaaact cacttcgaag agctggcgct
     3061 gcgctttatg gatatccgta aacgtatcta caagttcccg aaaatgggcg tgaaagcgaa
     3121 aatgatcgct gtcaccacca cttctggtac aggttctgaa gtcactccgt ttgcggttgt
     3181 aactgacgac gctactggtc agaaatatcc gctggcagac tatgcgctga ctccggatat
     3241 ggcgattgtc gacgccaacc tggttatgga catgccgaag tccctgtgtg ctttcggtgg
     3301 tctggacgca gtaactcacg ccatggaagc ttatgtttct gtactggcat ctgagttctc
     3361 tgatggtcag gctctgcagg cactgaaact gctgaaagaa tatctgccag cgtcctacca
     3421 cgaagggtct aaaaatccgg tagcgcgtga acgtgttcac agtgcagcga ctatcgcggg
     3481 tatcgcgttt gcgaacgcct tcctgggtgt atgtcactca atggcgcaca aactgggttc
     3541 ccagttccat attccgcacg gtctggcaaa cgccctgctg atttgtaacg ttattcgcta
     3601 caatgcgaac gacaacccga ccaagcagac tgcattcagc cagtatgacc gtccgcaggc
     3661 tcgccgtcgt tatgctgaaa ttgccgacca cttgggtctg agcgcaccgg gcgaccgtac
     3721 tgctgctaag atcgagaaac tgctggcatg gctggaaacg ctgaaggctg aactgggtat
     3781 tccgaaatct atccgtgaag ctggcgttca ggaagcagac ttcctggcga acgtggataa
     3841 actgtctgaa gatgcattcg atgaccagtg caccggcgct aacccgcgtt acccgctgat
     3901 ctccgagctg aaacagattc tgctggatac ctactacggt cgtgattatg tagaaggtga
     3961 aactgcagcg aagaaagaag ctgctccggc taaagctgag aaaaaagcga aaaaatccgc
     4021 ttaatcagta gcgctgtctg gcaacataaa cggccccttc tgggcaatgc cgatcagtta
     4081 aggattagtt gaccgatcct taaactgagg cactataacg gcttccacaa cagggagccg
     4141 ttttcttatg ccacttctta atgatctgct cgatttcagt gaccatccgc ttatgcctcc
     4201 gccctctgca caactatttg cagaacacct tcccaccgag tggatacaac actgcctgac
     4261 gctttctgct catgcgaccg ttcgccgccg tcgtttaccg ggggacatgg ttatctggat
     4321 ggtggtgcaa tgagccaatt accgatgttg ttcgccgtct gaacctgagc gcggatggcg
     4381 aagcggggat gaacctgctg gcccgcagcg ctgtcaccca ggcgcgtcag cgcgtggggg
     4441 ccgccccagt ggaatggctc ttccgccaga ccgcacagac aggggcgcgg aacgttacct
     4501 gaaggatgac tggcacggcc tgcaactttt tgccattgat ggcgcacagt tcaggacacc
     4561 tgataaaccc gagctgcgtg aatattatgg gtctgccaac acatccacta agcggcagaa
     4621 cgcctacccg gttatgcgtc tggtggcctt aatgaacctg ggaagtcaca ttctgctgaa
     4681 tgccgtgacc gcaccttacc ggcagagcga aactgtgctg gcccactcca tgctcgccac
     4741 catcccggat aactctatta cgttgtttga taaacttttt tacagcgaag acctgctgct
     4801 gacgctgaac cagaagggtt gtaaccgcca ctggctactg cctgcgtgga agaatatcgc
     4861 atcagaaatg atagagctgg gaaacacggc gtcaacaggg accatcccga aacgactgga
     4921 gcatcttcgg ggtgctctgg aagtggtgtt cataacaaaa cgcccccgac catcaaggcc
     4981 gaggtcggtt aagatatcaa aaacccgtta tcctgtgaaa catagtgccg ctcctcttaa
     5041 gtgaacggca ttgcccttct ggggccgtat tttttgttta cccaaagcaa cttttccata
     5101 aaccgacagc attagccttc atcatatttg cgacgatgta taacgcctaa acacagggat
     5161 attgtacttt acaggtcaca agtcaacgtc ggtgcttaag agccctgtga ggcgtatagc
     5221 ggcgttaaaa aactgccgag aagggtatat agcccggaag aagtgcgtaa aacgaactga
     5281 caggataaaa gtgccctgct caccctgtca gtaaagaaat tcttattaat cgtggcgatg
     5341 cctttcctga atagccgtta atgagccgac ttgtaacgcc tctttatagt gtttacggca
     5401 tacagaaacg tatcgttcat taccaccaat taccacctgc tcaccttcgt tataaggtct
     5461 gcctgcttga tcaagacgca gcaccatgct tgctttacgg ccacaaaaac agatggtttt
     5521 taattcaacc agtttgtcgg accatgccag taagtattgg ctgccaataa ataattcacc
     5581 tcgaaaatcg gtacgtaaac cataacaaag tacgggtata tcgagttgat cgacaacctc
     5641 cgataattca tatacttgtt gtctggttaa aaactggcat tcatcaacca gtacgcaatg
     5701 aattgcctgc tgttcatgtt ccgcacgaat ctcatcaaat aatgatgaat tttggttaaa
     5761 taattttgca ggcgatgaca aacctatacg cgaactgact ttcccggcac caaagcgatc
     5821 atcaatttct gccgtatata cgacagtgcg catgccgcgt tcctggtaat tgtatgaaga
     5881 ttgcaacaat gctgtagact tacccgcatt cattgcggaa tagtagaaat atagctgtgc
     5941 catcagccac aggccctcaa tgatatgaaa taaagttggc tggagtttat cataattcgt
     6001 cagtttttca gctcaacagt atgcacagaa gtgggtatat gcgttctccc ttacgaagcc
     6061 ttgcataatc cttctgagct atcattacaa ctgcctcgct tgttataagc gggtaaatga
     6121 ctgctggtaa ctattcacaa tctttaacct gttgcgcaag taatagccct ctgttgacct
     6181 ccaggagata gtgcaatact aagtccatgc tcttattgcg actgttctac ttttcatcat
     6241 tcgcttaata gggaattctc gtaaacacaa ctaatacaga agactgaaag gtcgtcagcc
     6301 tacgataatc tccccataaa atgtgacatg aatcaggaag ttttaacctc acgtgctgcg
     6361 aaatcatcgg tgtaaatagg gctatatgcc gcgtcttttc tggctaattt tatgaaaaga
     6421 tatttattgg cggcacaaaa taaagaacaa ttttgaattc cttacattcc tggctattgc
     6481 acaactgaat ttaaggctct attattacct caacaaacca ccccaatata agtttgagat
     6541 tactacaatg agcgaagcac ttaaaattct gaacaacatc cgtactcttc gtgcgcaggc
     6601 aagagaatgt acacttgaaa cgctggaaga aatgctggaa aaattagaag ttgttgttaa
     6661 cgaacgtcgc gaagaagaaa gcgcggctgc tgctgaagtt gaagagcgca ctcgtaaact
     6721 gcagcaatat cgcgaaatgc tgatcgctga cggtattgac ccgaacgaac tgctgaatag
     6781 ccttgcttcc gttaaatctg gcaccaaagc taaacgtgct cagcgtccgg caaaatatag
     6841 ctacgttgac gaaaacggcg aaactaaaac ctggactggc caaggccgta ctccagctgt
     6901 aatcaaaaaa gcaatggatg agcaaggtaa atccctcgac gatttcctga tcaagcaata
     6961 atcttttgta gattgcactt gcttaaaatc ccgccagcgg cgggattttt tattgtccgg
     7021 tttaagacaa tttaataaga ataagctata aaaaaacggc gtcgattgct caacgccgtt
     7081 tcgtggataa caccgatacg gatgttactt cttaatgccc atctcttctt caagccaggc
     7141 tttaaattcc gtgccaaggg tgttatgacg aataccgtat tcaacgaagg cctgcatgta
     7201 acctaattta ttaccgcagt catggctctt ccctttcata tgataggctt ccaccgtttc
     7261 tttttcaatc agcatatcaa ttgcgtcggt gagctgaatt tcatcaccag ctcccggagg
     7321 ggtttttgcc agcaacggcc aaatatccgc gctaagtacg taacgaccca caatagcgag
     7381 attagacggc gcaacatccg ctttcggttt ttctaccaca ccaaccatcg gtacgctttc
     7441 acccggcgct aattcaacgc ctttgcaatc cacaacgcca tatgcggtca catcagcaac
     7501 cggttcaacc atgatctggc tatgacccgt ttcatcaaag cggcggatca tctctgccag
     7561 gttatcctgt gacaaatcgg attcatattc atccagaata acatcaggca aaataacagc
     7621 taccggttca tcacccacta ccgggtgagc acacaatacc gcgtgtccca ggcctttcgc
     7681 cagaccctga cgaacttgca taatagtcac gtgcggtgga caaatagact gcacttcatc
     7741 aagcagttga cgttttacac gtttttccag cattgcttcc agttcaaaac tggtatcaaa
     7801 gtggttttca atagagtttt tagatgagtg tgtaaccagc acaatttcag taatgccagc
     7861 cgcaatacat tcattcacga cgtattgaat taatggctta tcgacaagtg gcagcatctc
     7921 tttcgggatg gctttcgtcg ccggcaacat cctggttcct aatcccgcaa cggggataac
     7981 ggcttttttg actttcgtat taatggcagc catttaaatt ctcctggact gttcgtgttt
     8041 tgaacgtgtt catatttctg tatcgcatcc cagtatatca gcaccccgaa ggggctaagt
     8101 tctgaaaagg aggcgagtta gcataattaa gacaattact tatacgaatg gcgacgatag
     8161 taccaccagc cgacattagc aggtaatgca aatttagccc gcgttatcgt ttgctcattc
     8221 tgcagacaac atcaagcgca gtcgaccacc ggttccccat atttggcatt gccaggcatc
     8281 gcatcgctgg ctcaattgat tcaaataagc gttacctaaa gtgcctaacg gaacaccatt
     8341 actgatttgc acctggtgtt cgccggtatt taacgtcgca ttcagacccg cagaaaccag
     8401 aatgagattt ttcagttcgc gatgataata gccaactaat agcggaaact gccccggcag
     8461 attggcctga cgaagtaaat ggtttacctg cttcaataac gcgcccaact ctggcaaccg
     8521 ttgattttgg tgtgcaagct gttcctgtaa taatccgtta aacaatgcgc gtaataacaa
     8581 ggcagcaagt acgccattat gtccagctcg ggtgacatca aggcaataaa atgccagatc
     8641 gttttccgaa agtgcggcaa tatcaagcac caggccgggt ttgtccgcgg caaccaattg
     8701 acgataatta acccggcaat gggaaatcac ctgctgaacc ggcggttgta gttcctgtaa
     8761 taatttcgcc gctgcggcag ggttatcaac cattgcatcc cagtcgcgaa aaagcctttc
     8821 ctcttcctca acgcgcgaat taaacatgct gggatagaga caggcaaaaa ccatctcgcg
     8881 caagcgattc aaatctttaa ctggtttcag caaaacatct tcaacgccca gacgtaacgc
     8941 tttggcaata tctgccatat tttcggtggc agatatcacc agaaccggtg tctggtcacc
     9001 tctgttgcgt atatgttcca gcagtttaag cccgttcatt cgtggcatcg cgatatcaca
     9061 tatcatcagg tctggagtga aacctcccag caactcaagg gcatccaccc catcagccgc
     9121 cagtaccgtt gtcgctccca atgaggaaaa ccacgaatcc agaagcgagc gaaataccgg
     9181 ctcatcttca acaatgagaa tctgttttcc gaccaatggc tgcgtcatgt tctctcccct
     9241 gactggcttt actcaatagt ggcatgctat tgccacctgc gcctgtcaga attaacttaa
     9301 gtgtagataa aaattctggt caaatgttgg tgcgtaccaa cggcaaaagt tcgtccattt
     9361 tcttttccac tgccagctgt ccggccgcaa tggcagcgtg cgcgcgatgg aaatcaagcg
     9421 tagatatttg cgggcaaaca ggttgaatca gaatatcggg cggatcacct gccatgcggt
     9481 tccttttaag gcggttctcc agcacctgga tagaagtggt cataatctct gttgccgttg
     9541 gcgctgtcac cgcccgacgt gtcgttatgc tgcctaacct ttctttcaga cgcgcatgcc
     9601 acggcagaga atcaccattc tcgctatttt cttcactgac attaaaggag agcaaatctt
     9661 gttgcatcaa atgagcatcg tgctgcaggt caaccgctat cacaatatca gcccccaatg
     9721 cacgcgtgag ggaaatagga attgggttaa cgactgctcc atcaaccagc cagtagccgt
     9781 tatgtgcaac aggtgccatg agtcctggaa tactgcatga tgcgcgaata gcaagatgga
     9841 gatcgccttc agtaaaccat aattcacgtc ccgtacttaa attggtggca acagccgcaa
     9901 agcgacggga acaattttcg atctctgttt ccggcattat ttcgcggtat tgattgaaga
     9961 cacgctcgcc gcgcagtaac ccaccgcgct gccaggagag atccatcagg cgtaaaacat
    10021 cccaataact gaaagaggtc acccaatctt ccagcgcaga taatcgatcg catgcatagg
    10081 cagcgcccac cagcgaacca attgaacatc ctgcaacgat atcaatttca atacccactt
    10141 tttttagcgc attaataacg ccaatatgcg accaacctct cgccgcgcca gatcccagcg
    10201 ccagccctat ttttatcttt ctcattattc ccgcaagatt cccctggaat gcaatcgtag
    10261 ccacatcgct actgctcggt taacatagtg ctactcaggc ggttaatacg ccgttattgt
    10321 ttccagggag attatttgtg tctcagcttt gtccctgtgg tagtgctgtc gagtatagcc
    10381 tatgttgcca cccttatgtg tctggtgaaa aggttgcacc tgatccagaa catctcatgc
    10441 gttcgcgtta ctgcgctttt gtgatgcaag acgcagatta tttaataaag acctggcatc
    10501 cctcttgtgg agcagcggcg ttacgtgccg aattgatggc cggatttgcg catactgaat
    10561 ggttgggatt gaccgtattt gaacattgtt ggcaggacgc cgacaatatc ggatttgtca
    10621 gttttgttgc tcgatttacc gaaggtggta aaacaggtgc aataattgaa cgttcgcgat
    10681 ttttgaaaga aaacggtcaa tggtactata ttgacggtac acgtccgcag tttggtcgca
    10741 acgatccctg cccttgtggt tcaggtaaaa aatttaaaaa gtgctgcggc caataatggt
    10801 tgacggtacg gtttagcaaa cactctcaac aaggtttttc cagcaatgca ttcactccaa
    10861 cgtaaagttc tgcgtactat ttgtccggac caaaaaggtc tgatcgcacg tattaccaat
    10921 atttgctaca agcacgagtt aaatatcgta cagaacaatg aatttgttga tcaccgtacc
    10981 gggcgctttt ttatgcgcac ggaactggaa gggattttta atgattccac cctgctggcg
    11041 gatctcgata gcgcattgcc agaaggctcc gtgcgtgagc tgaatcctgc cggtcgtcgc
    11101 cggatagtga ttctggtcac taaagaagcg cattgccttg gcgatttgtt gatgaaagcc
    11161 aattacggcg gcctggatgt cgaaatcgcg gcagttattg gtaaccacga tactttacgt
    11221 tctctggttg agcgttttga tattccgttt gagctggtaa gccatgaagg gttaagccgc
    11281 aacgagcacg atcaaaagat ggcggacgcc attgatgctt atcaatctga ctatgtggtg
    11341 ctggcgaagt atatgcgggt attaacgccg gaatttgtgg cacgcttccc gaataaaatc
    11401 atcaatattc accattcatt cctgcctgcg tttatcggcg cacgtcctta tcaccaggcc
    11461 tatgaacgtg gtgtgaagat tattggcgca accgctcact atgtgaatga caatctggac
    11521 gaaggcccaa tcatcatgca ggacgttatt catgtcgatc atacctacac agctgaagat
    11581 atgatgcgcg caggtcgtga cgtcgagaaa aacgtcttaa gtcgtgcgct atacaaagta
    11641 ctggcacagc gcgtctttgt ttacggtaat cggacgatta ttctttaatc gccagcaaaa
    11701 ataactggtt acctttaatc cgttacggat gaaaattacg caaccagttc atttttctca
    11761 acgtaacact ttacagcggc gcgtcatttg atatgatgcg ccccgcttcc cgataaggga
    11821 gcaggccagt aaaaagcatt accccgtggt ggggttcccg agcggccaaa gggagcagac
    11881 tgtaaatctg ccgtcatcga cttcgaaggt tcgaatcctt cccccaccac catcactttt
    11941 caaaagtccg taaactctca aacgaatccg caattaaata ttctgcccaa gcggggaagg
    12001 atgagaagct tcgaccaagg ttcgacttga gcgccagcga gagagcgttg ccgcaggcaa
    12061 cgacccgaag ggcgaagcgc gcagcgttga gtaatccttc ccccaccacc atcacttcct
    12121 taaataatca aaacctgtta ctccgagcta attttgcccg atggcaccac gtttaccggg
    12181 cctacaatat cctcccctgt aggtcggata agcgaagcgc atccgacaac tcaccccgca
    12241 ccgtgcccat aaaaaaaccc cgccgaagca gggtctatgt gaactaaaat tcgcttagtg
    12301 acgagcgcgc accagctggt acttacgtgt cagatactcc accggtacgc tccagatgtg
    12361 taccagacgc gagaacggga acagcaggaa taacgtcatc cccagcacca ggtgcagacg
    12421 gaagataaac gccacaccat cgaggtgttg agaagcgcca ccgtggaagg tcaccaccga
    12481 ctgcgcccag ccaaccagtt tcatcatctc gctaccgtcc atatgctgag cggagaacgg
    12541 aatggtcaac aggcccagcg cgcactggat aacgagcagc gacaggatca ggatatccgc
    12601 tccggtagtg gttgcacgca cgcgtgggct gaacagacga cgcttcagca acagcacgcc
    12661 gccaatcaga cacagcacgc cgctggcacc accagcaaac atcgccattt tctgtttcac
    12721 ttcaatcggc agccaggctt catacatcca gtgcggcgtc agcataccga agaagtgacc
    12781 aacaaaaatc cccagaatac cgatatggaa caggttcgac gccaggttca tccctttgcg
    12841 atccagcatc tggctggacg ccgcgcgcca ggtgtactgc ccgtagtcat aacgcagcca
    12901 gctaccaatc aggaagaccg ccccggctat gtacgggtag atatcaaaga agaacatatt
    12961 caggaattgc attagtgctg tcctccggtg gtgatattca gatattgcgg cgcgacggca
    13021 ccggcaaagc gacgctgatg agcagtgatt gctgaatcac cgcagccttt gtcagcaaag
    13081 aatttaacct gctcttcttc ccagacagca tccagcgcct gcggcgtatc atcgcgcgct
    13141 tcgtcggcaa ttttttccgc cactttgtcg ctgtcgatag cggtattggc caatttcagc
    13201 agcagatcaa acatcacggc ataacggctt tcacgctgtt gcagacgcgc gctcagcaat
    13261 gccagaatcg gcgcgatatc tttcaaacct tccacggctt cgctttgcgg cagctgcgac
    13321 aggtactcca gatacagcgg cagatggtcc ggtaattcgc ggctgtttaa ctgcaagccg
    13381 tgctgctcgt actgcgccag caggtccacc atcgcctggc cgcggtcacg ggattcaccg
    13441 tgaacatgtt caaatagcaa cagtgacgtg gcgcggccac ggtcgaacag ttcgctgtac
    13501 tgggcctggg catcgagcgg atccatcgcc gttaaatcgc gcaggaaaat gcccagcgca
    13561 tgggcatctt ctttcgacag atttttcgac gcagcaatcg cctcaaacat ctcttgttga
    13621 tgctgccata aggcagcatc cggatattca aggagacgcg atacaatcac gagttcgatc
    13681 atggatgcgg ctccgttttg ctggtcacat cgatggcatc gatacgacgg ctgttgaaca
    13741 gattgaattt ggtatctgaa ccgtggcagc catcaccaaa ggtaaagccg cagccatttt
    13801 tctccgggaa ggcttcccgt gccagttcac gatgactact cggcaccaca aagcgatctt
    13861 cgtagttagc aatcgccaga taacggtaca tctcctgtgc ctgggcttcg gtcagaccga
    13921 cctcttccag cgcacgggta tcaactttac cgtcaacggt ttcagcacgt ttgtaatgac
    13981 gcatcgccaa catacgtttc agtgcgcgca gtaccggttt ggtatcaccg gcggtcagca
    14041 gattcgccag atactgtacc ggaatacgca agctttcgac gtctggcaga atgccgttgc
    14101 tacccagctc acccgcgtct gctgcagact gaatcggaga cagaggcggc acgtaccaga
    14161 ccatcggcag tgtgcgatat tctggatgca gcggcagcgc cagtttccat tccattgcca
    14221 ttttataaac cggcgactgc tgtgcggctt caataacgct cagcggaata ccgtctttaa
    14281 tcgcctgctc gatgactttc ggatcgttcg gatcgaggaa cacctccagc tgacgctggt
    14341 aaagatcttt ctcgttctcg gtgctggctg cacgttcaat cgcgtcggca tcgtacagca
    14401 gcacgccaag ataacggata cgaccgacac aggtttctga gcacacggtc ggctgacccg
    14461 cttcaatacg cggatagcag aagatgcact tctcagactt accgctcttc cagttgaagt
    14521 agattttttt gtacgggcat ccggtgatgc acatacgcca gccacggcat ttatcctggt
    14581 cgatcaggac gatgccatct tcttcacgct tgtaaatcgc accgctcggg caggtcgcca
    14641 cacatgccgg gttcaggcag tgttcgcaca ggcgcggcaa atacatcata aaggtgtttt
    14701 cgaactggct atacatcgcc ttctggatgt tgtcgaagtt cttgtctttc gccagtttgt
    14761 caaactcacc acccaggtca tcttcccagt tcggcccttt ttcgattttc gccatccgtt
    14821 ccccggtaat cagcgaacgc ggacgggcaa tcggctgcga tttgctgcct tccggcgcgg
    14881 tatgcaggtt ctgatagtca aaatcgaacg gctcgtaata atcgtcgatc cccggcagat
    14941 gcgggttagc gaagatttta cccagcaaca tggcacggtt acccatgcgc ggctgcagtt
    15001 tgccgttgat tttacggatc cagccgcctt tgtatttttc ctggttttcc cagtcagtcg
    15061 ggaagccctg acccggcttg gtttccacgt tgttgaacca cgcgtattcc acgccttcac
    15121 ggctggtcca gacgttttta caggtaactg aacaggtgtg gcagccgatg cacttatcga
    15181 gattcagcac catgccgact tgtgaacgaa ttttcatttt acgctctcct gtacctggtc
    15241 attgccttcg ccatctaacc agtcaatgtt cttcatctta cgcactacaa caaactcatc
    15301 gcggttagaa cctacggtgc cgtagtagtt aaagccgtat gccagatggg catagccgcc
    15361 gatcatatgc gtcggtttcg gcgtgatacg ggtgaccgag ttatggatac caccacgctg
    15421 ttgggtaatt tccgaaccag gcaggttaac gatacgttcc tgcgcgtggt acatcatggt
    15481 catccctgcc ggaacacgct ggctgacaac cgcacgggca gtcagagcac cgttgctgtt
    15541 gaagacttca atccagtcgt tatcggcgat acccagatct ttggcatcgg cttcactcaa
    15601 ccagaccacc ggaccaccgc gacctaaagt cagcatcagc aggttgtcgc tgtaggtgga
    15661 gtggataccc cacttctggt gcggcgtcag gaagttgagc gctttttccg ggttgccatt
    15721 ggatttctgg cccatcactt ctttcaccga acgggtgtcg atcggcggac gataaaccag
    15781 caggctttca ccgaaatcac gcatccattg gtgatcctga tacagctgct gacggccaga
    15841 gagcgtacgc cacgggatca gctcgtgaac gttggtgtaa ccggcgttgt aagaaacgtg
    15901 ttcatcttca aggccagacc aggtcgggct ggagatgatt ttgcgcggct gtgcctgaat
    15961 atcgcggaag cgaatcttct cgtcttcttt attcagcgcc agatgcgtat ggtcacgacc
    16021 ggtaaactcg ctcagggcag cccaggcttt cacggctacc tgaccgttgg tttccggtgc
    16081 cagcgtcagg atcatctctg ccgcatcaat tgcggtgttc agcatcggct ggcctttcgc
    16141 cggaccttcc gctttggtgt agttgagctt acgcagcaga tccatctcgc tctgggtgtt
    16201 ccaggcaatc cctttaccgc cattaccgat tttctccatc agcgggccga tagaggtaaa
    16261 gcgttcgtaa gtcgccggat aatcgcgctc tacgaccata atgtgtggcg cggttttacc
    16321 agggatcagg tcgcactcgc cttttttcca gtctttcaca tccagcggct gcgccagttc
    16381 agcggcagag tcgtgctgga taggcagcgt gacgatgtcg gtttctttac ccagatgacc
    16441 gacgcacact tcggagaatt tcttcgcgat ggctttgtaa atttcccagt cgcttttcgc
    16501 ttcccaggcc ggatcgaccg ccgcagacag cgggtgaata aacggatgca tatccgaagt
    16561 attcatgtcg tctttttcgt accaggtcgc cgtcggcaaa atgatgtcgg aatagagaca
    16621 ggtgctcgac agacggaagt ccagcgtaac caccagatcc agcttgcctt ccagaccatt
    16681 gtcctgccag tccacttctt ccggcttcac gccgccctgt tgccccagat ctttaccctg
    16741 gataccgtgc tccgtcccca gcaggtactt gagcataaac tcatgacctt taccggaaga
    16801 accgagcagg ttagaacgcc agatgaacag gttgcgcggg tggtttttac cgttttctgg
    16861 ttgttctgcc gcaaaacgga tggaaccctc tttcagggat ttcaccgtat agtccaccgg
    16921 attcatcccg gctttttccg cttcgccagc gatagtcagc gggttagtgc ctaactgcgg
    16981 tgcagacggc agccagccca tgcgttccgc acggacgtta aagtcgatca agtgtccggt
    17041 atagcgggat ttgtccgcca tcggtgacag caactcttcc gccgtgacgg tttcataacg
    17101 ccactggctg gagtggttat agaaataaga agtgctgttc atgtgacgcg ccggacgctg
    17161 ccagtcaagg gcaaacgcca gcggctgcca gccggtttgc ggacgcagtt tttcctgacc
    17221 tacatagtgc gcccagccgc ccccgctctg accgacacag ccgcagaaaa tcagcatgtt
    17281 gatcagacca cgatagttca tatcgaggtg ataccagtgg ttcagccccg caccgacgat
    17341 aatcatcgaa cgaccgtgcg ttttatcagc gttatcggca aattcacggg cgatgcgaat
    17401 aatctggctg cgagaaacgc cggtaatctg ctcggcccag gccggggtat aagctttcac
    17461 atcgtcatag ctggttgcac agttaacgtc gttcaggcca cgttccagac cgtagtttgc
    17521 cagcgtcaga tcataaacgg tggtcaccag ggcggtgctg ccatcagcca gttgcaggcg
    17581 tttcaccggc agtttgtgca gcagcacgtt ttccagttcc actttgttga agtgttccgt
    17641 gccgtcgcca ccaaagtacg ggaagcctac ctcagcgatc tcatcctggc tacccagcag
    17701 gctcagttgc agctcggttt cttcgccagt tttgccgtcg cgctgctcaa gattccattt
    17761 gcccttctcg ccccagcgga agccaataga accgttcggc gcaaccattt cgccattggt
    17821 attaaaggcg acagttttcc attccggatt gttttcctgg cccagcgcat caaccagatc
    17881 agcagcgcgc agcatacgac ctgcagcgta gtaaccgtcg cgttcttcca gcatcaccag
    17941 catcggcatg tcggtgtagc gacgcacata gtcggtgaaa tactggcttg ggttgtcgag
    18001 gtggaattca cgcagcatta cgtggcccat cgccagcgcc attgccgcat cggtgccctg
    18061 tttcggtgcc agccacagat cgcacagttt ggcgatttca gcgtagtctg gtgtgacggc
    18121 aacagttttg gtccctttgt aacgcacttc agtaaagaag tgagcatccg gggtacgcgt
    18181 ctgcggtacg tttgaccccc aggcgatgat gtagctggag ttgtaccagt cagcagattc
    18241 cggtacgtca gtttgctcgc cccaggtttg cggagacgca ggaggcaagt cgcagtacca
    18301 gtcgtagaag cttaagcaag taccgccaat cagcgagaga tagcgtgcac ccgatgcgta
    18361 agaaaccatc gacattgccg gaattggcga gaaaccagca acacggtccg ggccgtagtt
    18421 tttgatggtg taaacgttag atgcggcgat cagttcgttc acctcctgcc aggaagaacg
    18481 aacaaatcca ccgcgtccac gcgcctgctt aaagcttttc gctttatcgg cgtcttcaat
    18541 gatagaagcc catgcctcaa ccggatcgct atgcagcgcc ttcgcttcac gccacatttt
    18601 catcaggcgt ttgcgcatca tcgggtattt caggcggttg gcactgtaaa gataccagga
    18661 gtagctggca ccgcgcgggc agccgcgagg ttcatggttt ggcagatccg gacgggtacg
    18721 cggatagtca gtctgctggg tttcccaggt gaccagaccg tttttgacgt agattttcca
    18781 gctgcaggag ccggtgcagt ttaccccgtg ggtagagcgg acgattttgt catgctgcca
    18841 acgctggcga tatccatcct cccagtcacg gttggtattg agaagctggc catgcccatc
    18901 ggcaaaggtt tcacccttct gtttgaagta gcgaaaccgg tccaggaatt tactcatcgg
    18961 ttttctcctg tgggagcctg tcggccctct gataaatcga cattgctaat gtttaagggc
    19021 aaggtaacgc tctgaaacag cgtgggaatt gataacgatc aagtgtgatg gggatgaaaa
    19081 ataaagtaaa ttcctaagat acccc
//
LOCUS       DAZGJW010000086        18916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZGJW010000086 DAZGJW010000000
VERSION     DAZGJW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.086"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            144..710
                     /gene="yqaB"
                     /locus_tag="ACHKIK_003074"
     CDS             144..710
                     /gene="yqaB"
                     /locus_tag="ACHKIK_003074"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO9084197.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKYHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            707..1135
                     /locus_tag="ACHKIK_003075"
     CDS             707..1135
                     /locus_tag="ACHKIK_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO9084198.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1208..2764
                     /gene="gshA"
                     /locus_tag="ACHKIK_003076"
     CDS             1208..2764
                     /gene="gshA"
                     /locus_tag="ACHKIK_003076"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO9084199.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            2914..3429
                     /gene="luxS"
                     /locus_tag="ACHKIK_003077"
     CDS             2914..3429
                     /gene="luxS"
                     /locus_tag="ACHKIK_003077"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO9084200.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGKGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3493..5031)
                     /gene="emrB"
                     /locus_tag="ACHKIK_003078"
     CDS             complement(3493..5031)
                     /gene="emrB"
                     /locus_tag="ACHKIK_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO9084201.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5048..6220)
                     /gene="emrA"
                     /locus_tag="ACHKIK_003079"
     CDS             complement(5048..6220)
                     /gene="emrA"
                     /locus_tag="ACHKIK_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO9084202.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6347..6877)
                     /gene="emrR"
                     /locus_tag="ACHKIK_003080"
     CDS             complement(6347..6877)
                     /gene="emrR"
                     /locus_tag="ACHKIK_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO9084203.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(6968..7303)
                     /gene="ygaH"
                     /locus_tag="ACHKIK_003081"
     CDS             complement(6968..7303)
                     /gene="ygaH"
                     /locus_tag="ACHKIK_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO9084204.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7293..8030)
                     /gene="ygaZ"
                     /locus_tag="ACHKIK_003082"
     CDS             complement(7293..8030)
                     /gene="ygaZ"
                     /locus_tag="ACHKIK_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO9084205.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8154..9338)
                     /locus_tag="ACHKIK_003083"
     CDS             complement(8154..9338)
                     /locus_tag="ACHKIK_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9084206.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     LLGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFINDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9530..10522)
                     /gene="proX"
                     /locus_tag="ACHKIK_003084"
     CDS             complement(9530..10522)
                     /gene="proX"
                     /locus_tag="ACHKIK_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO9084207.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10580..11644)
                     /gene="proW"
                     /locus_tag="ACHKIK_003085"
     CDS             complement(10580..11644)
                     /gene="proW"
                     /locus_tag="ACHKIK_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO9084208.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11637..12839)
                     /gene="proV"
                     /locus_tag="ACHKIK_003086"
     CDS             complement(11637..12839)
                     /gene="proV"
                     /locus_tag="ACHKIK_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO9084209.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13194..14153)
                     /gene="nrdF"
                     /locus_tag="ACHKIK_003087"
     CDS             complement(13194..14153)
                     /gene="nrdF"
                     /locus_tag="ACHKIK_003087"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO9084210.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14163..16307)
                     /gene="nrdE"
                     /locus_tag="ACHKIK_003088"
     CDS             complement(14163..16307)
                     /gene="nrdE"
                     /locus_tag="ACHKIK_003088"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO9084211.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYPNQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16280..16690)
                     /gene="nrdI"
                     /locus_tag="ACHKIK_003089"
     CDS             complement(16280..16690)
                     /gene="nrdI"
                     /locus_tag="ACHKIK_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO9084212.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(16687..16932)
                     /gene="nrdH"
                     /locus_tag="ACHKIK_003090"
     CDS             complement(16687..16932)
                     /gene="nrdH"
                     /locus_tag="ACHKIK_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO9084213.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17180..17509)
                     /locus_tag="ACHKIK_003091"
     CDS             complement(17180..17509)
                     /locus_tag="ACHKIK_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO9084214.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            17661..18005
                     /locus_tag="ACHKIK_003092"
     CDS             17661..18005
                     /locus_tag="ACHKIK_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO9084215.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(18042..18491)
                     /gene="alaE"
                     /locus_tag="ACHKIK_003093"
     CDS             complement(18042..18491)
                     /gene="alaE"
                     /locus_tag="ACHKIK_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO9084216.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPANILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         4742 a         4770 c         4883 g         4521 t
ORIGIN      
        1 ctcccaaaaa tttccttcaa aaatatctca tttctaaata actactttat ccatttcctg
       61 aattttcttc tccttcgcca tcagcgacaa aatcaagcaa ttacgctaaa gtagcgtcat
      121 atttttcgac ttgcgagaag accatgtacg agcgttatgc aggtttaatt tttgatatgg
      181 atggcacaat cctggatacg gagcctacgc accgtaaagc gtggcgcgaa gtattagggc
      241 actacggtct tcagtacgat gttcaggcaa tgattgcgct taatggatcg cccacctggc
      301 gtattgctca ggcaattatt gagctgaatc aggccgatct cgacccgcat gcgttagcgc
      361 gtgaaaaaac agaagcagta agaagtatgc tgctggatag tgtcgagccg cttcctcttg
      421 ttgaagtggt gaaaagttgg catggtcgtc gcccaatggc tgtaggaacg gggagtgaaa
      481 gcgccatcgc tgaggcattg ctggcgcacc tgggattacg ccgttatttt gacgccgtcg
      541 tcgctgctga tcacgtcaaa taccataaac ccgcgccaga cacatttttg ttgtgcgcgc
      601 agcgtatggg cgtgcaaccg acgcagtgtg tggtctttga agatgccgat ttcggtattc
      661 aggcggcccg tgcagcaggc atggacgccg tggatgttcg cttgctgtga gtgaagcgtt
      721 atcgcttttc tcgttgtttg ccagtagttt tctcagcgct acactattac ccggcaactc
      781 cgaagtcgtg ctggtggcaa tgttgctttc cgggatcagt tatccctggg ttttagtttt
      841 aacagcaaca atgggtaata gccttggagg gttaactaac gttatccttg ggcgtttctt
      901 tccattgcgt aaaacatcgc gctggcaaga gaaagctacc ggctggctga aacgctatgg
      961 tgcagtcacg ctattattaa gctggatgcc cgtggttggc gatttactgt gtctgttagc
     1021 gggatggatg cgcatctcct gggggccagt aatctttttt ttgtgccttg gtaaagcgtt
     1081 acgctatgtt gcagttgcag cagcgaccgt tcagggcatg atgtggtggc actaattgta
     1141 ggcctgcaca tatggtcacc attacagtta tgctaattaa aacgattttg acaggcggga
     1201 ggtcaatttg atcccggacg tatcacaggc gctggcctgg ctggaaaaac atcctcaggc
     1261 gttaaagggg atacagcgtg ggctggagcg cgaaactttg cgtgttaatg ctgatggcac
     1321 actggcaaca acaggtcatc ctgaagcatt aggttccgca ctgacgcaca aatggattac
     1381 taccgatttt gcggaagcat tgctggaatt cattacacca gtggatggtg atattgaaca
     1441 tatgttgacc tttatgcgcg atctgcatcg ttatacggcg cgcaatatgg gcgatgagcg
     1501 gatgtggccg ttaagtatgc catgctacat cgcagaaggt caggacatcg aactggcaca
     1561 gtacggcact tctaacaccg gacgctttaa aacgctgtat cgtgaagggc tgaaaaatcg
     1621 ctacggcgcg ctgatgcaaa ccatttccgg cgtgcactac aatttctctt tgccaatggc
     1681 attctggcaa gcgaagtgcg gtgatatctc gggcgctgat gccaaagaga aaatttctgc
     1741 gggctatttc cgcgttatcc gcaattacta tcgtttcggt tgggtcattc cttatctgtt
     1801 tggtgcatct ccggcgattt gttcttcttt cctgcaagga aaaccaacgt cgctgccgtt
     1861 tgagaaaacc gagtgcggta tgtattacct gccgtatgcg acctctcttc gtttgagcga
     1921 tctcggctat accaataaat cgcaaagcaa tcttggtatt accttcaacg atctttacga
     1981 gtacgtagcg ggccttaaac aggcaatcaa aacgccatcg gaagagtacg cgaagattgg
     2041 tattgagaaa gacggtaaga ggctgcaaat caatagcaac gtgctgcaga ttgaaaacga
     2101 actgtacgcg ccgattcgtc caaaacgcgt tacccgcagc ggcgagtcac cttctgatgc
     2161 gctgttacgt ggcggcattg aatatattga agtgcgttcg ctggacatca acccgttctc
     2221 gccgattggt gtagatgaac agcaggtgcg attcctcgac ctgtttatgg tctggtgtgc
     2281 gctggctgat gcaccggaaa tgagcagtag cgaacttgcc tgtacgcgcg ttaactggaa
     2341 ccgggtgatc cttgaaggtc gcaaaccggg cctgacgctg ggtatcggct gcgaaaccgc
     2401 gcagttcccg ttactgcagg tgggtaaaga tctgttccgc gatctgaaac gcgtcgcgca
     2461 aacgctggat agcattaacg gcggcgaagc gtatcagaaa gtgtgtgatg aactggttgc
     2521 ctgcttcgat aatcccgatc tgactttctc tgcccgtatc ttaaggtcta tgattgatac
     2581 tggtattggc ggaacaggca aagcgtttgc agaagcctac cgtaatctgc tgcgtgaaga
     2641 gccgctggaa attctgcgcg aagaggattt tgtagccgag cgcgaggcat ctgaacgccg
     2701 tcagcaggaa atggaaaccg ctgataccga accgtttgcg gtgtggctgg aaaaacacgc
     2761 ctgacagaaa agaaaaaggc cactcgtgag tggccaaaat ttcatctctg aattcaggga
     2821 tgatgataac aaatgcgcgt ctttcatata ctcagactcg cctgggaaga aagagttcag
     2881 aaaattttaa aaaaaattac cggaggtggc taaatgccgt tgttagatag cttcacagtc
     2941 gatcataccc ggatggaagc gcctgcagtt cgggtggcga aaacaatgaa caccccgcat
     3001 ggcgacgcaa tcaccgtgtt cgatctgcgc ttctgcgtgc cgaacaaaga agtgatgcca
     3061 gaaagaggga tccataccct ggagcacctg tttgctggtt ttatgcgtaa ccatcttaac
     3121 ggtaagggtg tagagattat cgatatctcg ccaatgggct gccgcaccgg tttttatatg
     3181 agtctgattg gtacgccaga tgagcagcgt gttgctgatg cctggaaagc ggcaatggaa
     3241 gacgtgctga aagtgcagga tcagaatcag atcccggaac tgaacgtcta ccagtgtggc
     3301 acttaccaga tgcactcgtt gcaggaagcg caggatattg cgcgtagcat tctggaacgt
     3361 gacgtacgca tcaacagcaa cgaagaactg gcactgccga aagagaagtt gcaggaactg
     3421 cacatctagt cagtaaacta tcttcacaat taattgaaaa aagccagttc aaatgaactg
     3481 gcttagttgt acttagtgcg caccgcctcc gccgccacct gcgccaaatg gcggtttagc
     3541 aaaccacacc agccccagca gtacgaggaa tattccggct gacatccaga agatctcatt
     3601 ggcggaaata atcagcccct gattggtgat ctgctgggca atccagcctg atgcctgctg
     3661 ttgcgtcatc ccaagccctt ccagttgact gtacatcgcc tgggcattcg ggttgaacgg
     3721 gtttaccgac tcagtcaact gcgcatggtg catcgactcg cggttggtcc acatggtcgt
     3781 ggttatcgac gtgccgatag accccgccag cgttcgcgta aagttagaga ggctcgatgc
     3841 cgccgccagt cgttccggtg gcaaaccaga cagcgtaatg gtggtcagcg gcataaagaa
     3901 gcaggccacc gcaaacccct ggataaactg cggccaggcc gacgcgccaa aatccatacc
     3961 tggttcaaag gtataggcac gccagtagaa gcagacggca tacataataa agctgaaggt
     4021 taccagccgc cgcatatcca gtttatgcgc gaagcggccg ataatcggcg acaggatcac
     4081 cggaataatc cctaccggcg cagaggccaa acctgcccag gtcgccgtgt aaccgtagac
     4141 ctcctgcaac aactgcggca gcagaacaat agcgccgaag tagagcatat acgcgaggct
     4201 gatacacaag cagccgatgg tgaagttgcg cgacttaaac aacgacagat cgactatcgg
     4261 gttatcgtcg gtcagctccc agacaatcag gaagcagata gccaccaccg ccaccacggt
     4321 aaggatgata atttcctgtg atgaaaacca atccagctct ttaccgcggt caagcataat
     4381 ctgcaggctg ccgataccaa taaccagcag tgccagcccc acggcatcaa tccgccgccg
     4441 ttcggtgcgg gtttcacgtc cgcgcagagt ttgcagtgtc atcaacacca ccgccacgcc
     4501 aatcggcacg ttgatgaaga atatccagcc ccagtggtaa ttatcgctga tataaccgcc
     4561 gaggatcggg ccgcaaattg gcgcgacaat caccgtcatc gaccacaacg ccagcgcgat
     4621 cgagcgtttg gctggcgggt agttattcag caatagactt tgcgaaagcg ggatcaacgg
     4681 cccggcgaca atcccctgaa tcacgcggaa gaagatcagc atattcaggc tgctggagac
     4741 accacacgcc cacgacgcaa tagcaaaggc gatggtggac caaaggaaca gtttcacttc
     4801 cccgacgcgc tttgccagcc agccggtaag cgggatcgag atggcattcg ccaccccgaa
     4861 agaagtgatt acccacgttc cctggctgag cgatgagccc agattcccgg cgatagtggg
     4921 gatcgccacg ttagcaatgg tggagtccag cacctgcatg aatgtcgcca gtgacagcgc
     4981 aatcgtcata atgaccagtt gcgcgccttc cagcggtttt tgctgttgca tcacacgcac
     5041 ctctggatta gccagcgtta gcttttacga tatcgtcgat cagtttattg acaggtgcca
     5101 ggctgatttc acgcgcggta cttaccgcga ccggtgttga acgtacttta tttgccagta
     5161 cctgaccgtc acggttagtg gtattgacgc tcaccagcgt ggacaaacca atgcgcagcg
     5221 ggtattgctc cagctgtttc tggtccagtt cgatacgcac aggtagacgc tgaacgactt
     5281 tgatccagtt accggtcgca ttttgcgccg gaagcagtga gaatgcgcta cctgtgccca
     5341 tatccagacc aaccacttta ccggtgtatt tcacatcatc gccgtaaata tccgtggtga
     5401 tagtgaccgg ctgaccgata cgcatattgg caatctgcgt ctctttaaag ttggcatcca
     5461 cccacatatt ggtggctgga acgaccgcca tcagcggcgt cgttgggcta atttgcgccc
     5521 caggctgtac cgcgcggcgg gagacataac cggtcatcgg actgacaata cgggtacgct
     5581 ccagcgccag ccaggcgtta cgtacttcgg tggcagcctg ttgcacggca ggctgatctt
     5641 ccagtttagt ccccagaatc atcgcctgat tggcattgta ttgttgaata gcgacgtcca
     5701 gttgcgcctg ggcactggtg acggcgtcgc gggcgtgttg cagctcttcg cgaccaatca
     5761 ggttggcatt gcccagcggc acacggcggt tgtagtcgct ttgtgctttc gcgagggcga
     5821 ttttttgcac ctcaatattc gcctgcaact gtttgctgtt aatcatcagc tggtgggttt
     5881 ggcgaacgct ggaagccagt gcagttttgg ctttttcaaa cgcctggcga gcatctgtcg
     5941 ggtcgagagt gaccagaaca tcgccttctt ttacaaaatc ggtgttatcg gcccagactt
     6001 tcgtcacgct gccagacacc tgagacataa tttgaatctg attccctgcc acgtatgcgt
     6061 catcggtttc ttcgaaatga cgcagtacca aaaaccaata aatccctatc gctacggcaa
     6121 taattataaa gagcaaggtg agaaggagga gcagacgctt acgtttgccg ctcttcttta
     6181 ccggttgctg cggggtttga gtctccgcat ttgcgctcat attgttctcc acgatcttct
     6241 taactcatcg gctgagccga gctgcttgcc aaaaaggcca gcatgttgcg atgctggccg
     6301 gtcatttttt cttttataaa tctggatttt tgagcgagat gacgcgttag ctcatcgctt
     6361 cgagaaccac accgtcttgt tccatctggt cgagacggga gagcaatttg cgggtgattt
     6421 gctcgagctg atctttttct gttgtgctga gcgcggacca gagttgatgc aggcagttat
     6481 gctgcggtgg taaaacctcg cgcaaaaact cgtgaccttt ttccgttaat tgcagatgca
     6541 ggcagcggcg atcgttatcg ctttcacgac gttcgatcca accgcgtttt tccagttcat
     6601 cggcaatacg cgtcgcgttg gtacgggatg atccaagagc acaacttaat tcagaaggct
     6661 gaatactgtg gttttcctga gactccagcg taatcaacgc cataaacaac gtctcgttaa
     6721 tcccctgagc cttcagcatt ttattgcggt tctccagcag cttgctttgc atgtgcatgc
     6781 aaagacgagt cagaaggatc tcctgataag gaaaatcttc gtggcggctg gcgcgaaatt
     6841 ttagcatttg ttcaatgggc gtaaacgaac tatccatttg ggtatgacct cattaatttc
     6901 agccgatata gtaacgacag tgacaaataa agtaaatgta ttgttttaga aaaatgattc
     6961 ttgtgggtta tataatcgcc atcactttcc aggcgagccc ataggccagc gcactaagca
     7021 gtgttgggat gataatgctg cgtgttttat agaaactggc acccagtacc gcgaagccga
     7081 ccagcgtggg cacgaaacgg cgtgtatcgt gcatcacttc tggtgcggta gagacaacca
     7141 gcagagcgca tatcgaggcg atgccaatgg tgtcgagcaa aatacctacc gcgccacgtt
     7201 tggttgggcg ggcattaccc acacgcaggc gcagcggcaa atagcggaag caataattcg
     7261 ccacgccaac cagtaaccca agcagcagaa cctcatagct catcgggcgc tccttgccag
     7321 aatgcctgga ttaacgcagt gaggcagcca cagacaatgc ctgccagaat ggcgacggga
     7381 atagaaaata gcgttacgcc tgcaagggca ccaactaacg ctgcggtaac gcaaagagat
     7441 tgtttgcgct ggaaagaggc gagcaggaaa ctcataaaga gtgccggaag cataaaacct
     7501 aatgcagctt caacggcggg ataaccttgc agcaagccgc tgccggagaa tgcccctatt
     7561 accgtaccaa atacccacga tgaccatgaa ctgaaggcaa tgccgatcat ccagttctcg
     7621 ctccagcggc gattattgcg taccagtttt gcggttgcgg cggcaaaaac ctcatccgtc
     7681 aggccaaatg cccacagggc ggttttcgat ttttgcagac gctgaataat acggctacgc
     7741 agtgacgggc catacaacac atggcgaaca tccattgcca tgacggtcag tgcagcaacc
     7801 cacaaactac tcccggctgc cagcatcgcg gtaatgacga actggctcgc gcctgcataa
     7861 atgatgcagg agaaaaaaac gctttcgaga ggagagaatc ccagacgggt cgcattcaga
     7921 ccgaacgcaa aggccaccgg aatataacta ataacaatcg gtaaactgtc tttgcatcct
     7981 tccatgaagg tcgccgaacc aggagcaggc tgtggagtag ggctttccat agagtgtacg
     8041 cttaacgatt gttaatatta taaatagact gaatgaatat cttaacctta tcagactgat
     8101 gggcttctta acacccttat aagtgtaaag ccacgaaaac ggttgctgat tgcttaattt
     8161 gcggcttcct gacgatgaaa acctcgccac cagaccagta agttaaccag ggcaatcgtc
     8221 gcgccagcca gacaaacgcc agcccaaccg ccatgttgcc aggctgaggc tgaaattagc
     8281 gaaccggcgg caccgccaat aaagtagctg gtcatgtaac ctgcggtcag gcgattacgc
     8341 gcatcagggt gtattcgata aattaccgtc tggttagtga tatgcacgcc ctgtacggtg
     8401 agatccagca ccaggattcc gataatcaac gccagtacgg aagtgtgacc aaaccagatc
     8461 gccagccatg aaagtaatag cagcagcaga ccgaaagttg tggtgtggtg cgatttaccc
     8521 ttatcggcaa aaccgcccgc cggacgagcg cccaacgcgc cggcagctcc cgcaagtcca
     8581 aacagaccaa tgacaccatc gctgtagtta aaaggtggag cggcaagcaa aaaggccatt
     8641 gaggtccaga gaatgctgaa gttggcaaag gtcaggcagc caagcaatgc gcgggtgcgc
     8701 aggatcttat cgttgataaa catgctgaaa acggaaccca acaactgtgg gtagttgagg
     8761 tgggtttctg atttcatttg tggcagacca cgccataatg ctagcgccat cagagccatt
     8821 aacaccgagg caacccaaaa gacggtgcgc cagccgccga ggtttgccag taacccggca
     8881 acggttcgtg ccagcaatat ccccaacagc agaccgctca taatggtgcc aaccactttg
     8941 ccgcgtttat ccggtgaagc cagcgtcgct gccagcggaa ccagaatttg tgccacgaca
     9001 gagaataaac cggttaatgc cgtaccgagg agcatcatcg ccagcgactg actgctggcg
     9061 gtaatcaaca taccgcccgc tgccagtaag gtcatcgaga caatcaggcg gcggcgttca
     9121 aacatatcac cgaggggaac aagaaacagc agacctgctg catagcccaa ttgcgcggcg
     9181 gtaacaataa agcctgccga actggcggaa agggaaaagt tacgcgcgat ggtgtcgagt
     9241 agcggctggg cgtaatagtt actggcgacc gccagaccgg tggcgataga catcagcacg
     9301 atcagcgccg ggctaagctc atgattaggt ttagtcatta tttttgttca tcaggtgatg
     9361 attaagctgg aaatcaataa tagcggatgt tgatgaatat gggatgaaag atagcttgtt
     9421 gaaatgtgcg atgtggtttg taggcatgat aagacgcgtc agcgtcgcat caggcattgt
     9481 gtacagagcg gcggatgagg caaaaacgcc ttatccgccc gaataaaaat tacttctgcg
     9541 ctgccagcgc ctcattcacc cagccatcga actgctgctg gtgggctttg atccagccat
     9601 caacatggcc ctgaatatcg ccttctgagg ctttgccgtc atgcataatg gcgttctggg
     9661 cgttaatatc tgccactggc aactgcataa tggcaaacag tttcgctgct gccgggtttt
     9721 tctcggccca ggctttgttg gcaacgatat gcatggtgct gaccgggaag ccataattcg
     9781 caccattcgg cagtttggta tcggcgtttt tatcgcccgg cagtgcggag aacggcacct
     9841 gcaaccagac cacatctttc cctggcttca gctcattact cacccagtac ggcgtccagg
     9901 tgtagtaaaa caccggtttg ccctctttgt agcgactgat ggtgtcggcc atcatcgccg
     9961 cgtagttccc ctgattatgc gtcacggtat gggtcagttc atacgcggca agctggtggt
    10021 tgatcgcacc ttcgcagccc cagccagggt tacaaccggt taaatccgct tttccgtcgc
    10081 cgttggtatc gaacagtttg gcgatcttcg gatctttcag ttgtgcgatg ttggtgattt
    10141 tgtactggtc ggcggttttc ttatcgatca ggtaaccctg tgccgcgccg ttaacaaata
    10201 ccccttcacg ataaaatttc ttatcgccac cggcagcttc gtacatgttg tcatgcagtg
    10261 gcgtccagtt cacggcggtg aaggttgcat cgccggaagc aagcgaggtg tagccaacgt
    10321 tgtaatccac ttcgctgggt ttgttgacgg tataacctaa tttctccagc gcacgactga
    10381 ccagcagcgt ctggaaggtt tcttcagtga tggtgctctg aactggatta acagtaatgc
    10441 ctttgcccgg cagatcggca gcaaaagttt gtgtagagat aagcgtggca aacgctgtcg
    10501 caaaaagtac gctatgtcgc attgttattc ctttttttgg cagggtgata atgcccggcg
    10561 tcaccgggca agtgcagagt tacttaatga atgggcgggt cagcagacca acagggccag
    10621 tggtgtacca gcgacggttg ccgcgactgc gtgagtcgcg cccaacggcc tgcgtcaggc
    10681 gatcgaggat aatggcgagg atcacaatcc cgacgccgcc aacggtggca agccccatat
    10741 ccagacgacc gataccgcga agtaccatct gacccagccc gccgacggca atcatcgagg
    10801 caatgaccac catagaaagg gccagcatca gcgtctggtt aacgcccgcc ataatggtcg
    10861 gcatcgccag cggtaactga actttgaaca gcatctggcg cgggctggca ccgaatgagc
    10921 gcgaggcttc aatcagatcc gccggaacct ggttaatccc cagaatggtc agacggataa
    10981 tcggcggcag agcaaagatg atcgtcacca ccacgcccgg cacgttaccg ataccaaata
    11041 gcatgacgat tggcaccaga taaacaaacg ctggcgtggt ctgcatggca tcaagcagtg
    11101 gacgaataat tttcgccgct cgcggacttc tcgccagcca gatccccagc ggcaaaccaa
    11161 tgacgataca gaacagcagg gcggttaaca ccagcgccag ggtaaccatg gcctgcgacc
    11221 aggcaccgat tgcgccgatg gcaatcagcg aaaccagcgt cgccacgccc attccgaccc
    11281 cggaaatctg ccaggcgatg agagcgaaaa cgataatcgc caccggtgcg ggcataccca
    11341 gcagcaattg ctggaaaccg ttgagaatgt aatcaaccgg aacgcgcacg ccctggaaga
    11401 cgggacggaa atgggtaaca acccagtcga tcccttcagt gacccaactg tcgagcggga
    11461 tcagcgtttt atggaacgga tcgagaatat taaaatgctc gacgtttggc gcaggcgtac
    11521 tggtcagcca gtcagcacca ccgccgtcag tcggtgcagt cgccggtgta ccccaggcgt
    11581 ctgcggattg cacggcactg tccgccgctg gcgtggtatc ccacggatta ttttgatcag
    11641 ccattattta ccccctcacg atctaaagcg cgcagcagca ttcctttcga aatgatgccg
    11701 acatactgtt ggtcctcgtc gaccacgggc accgcacagg gtgcctgtcc gacatgagag
    11761 agcaactcgc taagaggcgt ttgtgcatcg actgctaacg gcgcatcaat cagcgccgca
    11821 tcaagacctt gctgctgcgt taacgcggtt ttaagcgaat cgatggaaac tgcgccgaca
    11881 aacttattac cgcgttcgat aacgtagcca tattcgcgat cttcatcctg caataatttc
    11941 agtgccgaac gtgggccgaa gccaggggtt ttacgaatta agccattcgg tgtccggcgg
    12001 gcaatatctt tcgcactgaa tacctgacta atatcaacgc cacggaagaa ggtacggaca
    12061 taatcattcg ccggattatt gagaatttca tccggtgtgc cgacctgtac cacttcacca
    12121 ttttgcataa tggcaattcg gtcgccaata cgcatagcct catcgagatc gtgggaaata
    12181 aagacaatgg tgcgctgatg tttcgcctgt aattttacca gctcatcctg catctcggtg
    12241 cgaattaatg gatcgagcgc cgagaaggct tcgtccatta ataatatatc cggattaatc
    12301 gctaacgcgc gggctaatcc cacacgttga cgcatcccgc cagagagttc atccgggtag
    12361 ctgtgggcat aattttccag cccgacctga cgcagtgcat caagggcttt ttcccggcgt
    12421 tcttcggcat taattccggc caattccata ccgaacgcag tattgtccag cacggtcata
    12481 tgcggcatta aggcaaagga ctggaagacc atcgcaatct tttttctgcg cacctcacgg
    12541 agttcggcat cggatatttt ggcaatatcc acaccatcaa tcagcacttg cccgcgggtg
    12601 ggttcaatca ggcgattgag aaggcgtacc attgtggatt tacccgagcc ggataatccc
    12661 atgatgacaa atatctcgcc ttcttcaatg gccagactgg cgtcttttac gccaagcgat
    12721 agcccagttt tttccagaat ttgttctttc gaaagtcctt gttcgatata tttgaacgct
    12781 cgctgtggat gctcgccaaa tattttataa agatttttaa tttctaattt aattgccatg
    12841 caatagaaag attcctttat ttgtttatgt cgatatatta cccaatggaa atagtcactt
    12901 ttttctaccc taacatactg agaatctgag gcaacccctg agggcaaatg tggcatgaat
    12961 attgatgcga gcaaatcgcc ggaatttccc gtgatataag ggctgagagc aaatcgagaa
    13021 aagttgatat tttttgtcat agatagcgaa aaaacgcctg aatattgtag tttcaggcga
    13081 ataatgcaac agagtatcag tgtagatcac tacagaatat ttgcgtgata aaaagctatt
    13141 tggcgggaat tatttccctg ctgcgggtag tgatattttt gaaaataaca ccctcagaaa
    13201 ttccagtctt catcttctgt ttcaaccgct ttccccatca cataagagga gcctgaaccg
    13261 gaaaagaaat cgtgattttc atcggcattc ggcgaaagcg cggcgaggat tgccggattc
    13321 acttccgcca tttctgcggg aaataacggt tcgtagccca gattcatcaa agccttattg
    13381 gcgttgtaac agagaaacgc tttcacatcg tcagcccacg gggtttcggc gtacagctca
    13441 tcggtgtatt gcaactcgtt gtcgtagagt tccagcaaca aatcgaaggc gaaactcttc
    13501 aactcttcac gttgtcccag agatatcttt tccatgtttt tctgatattt atagcctatg
    13561 tagtaaccgt ggactgcttc atcgcggata atcagacgga tcagatctgc ggtattggtc
    13621 agctttccgc ggctggaaaa atacatcggc agccagaaac cggaatagaa caaaaaagat
    13681 tcaagaaaca cactggcgat tttctttttc agcggatcat caccgcgata atgttgctga
    13741 ataatctgag cttttcgctg caacggtgcg ttttcttcac tccaggcgta ggcggcatcg
    13801 acatctttgg tctgacacag cgtcgagaaa atcgaactgt aagagcgggc atgaaccgct
    13861 tccataaagc tgatattcga taatactgcc tcttcatgag gcgtgagcgc atcgggcatc
    13921 agcgaaggcg cgccgataac attttgcagc gtgtcgagca gcgtcaggcc agtaaaaacg
    13981 cgcattgtca gttgttgttc tacgacactt aatgtctgcc atgcaggtat atcgttcgac
    14041 agtggcacct tttctggtag ccagaaattg ctggtcaggc gattccacac ctccagatct
    14101 ttatcgtcag atatcttgtt ccagttgatg gcgctgatac gtgagagttt catagatatt
    14161 ccttaaagtg cacaggagac gcagccttca atttcagtgc cttccagcgc catctgacgc
    14221 aggcgaatgt aatagagcgt tttgatacct ttgcgccagg cgtaaatctg cgctttgttg
    14281 atatcgcgag tggtggcggt atcggggaaa aacagcgtca gcgacaaacc ctgatcgaca
    14341 tggcgagtcg cttccgcgta ggtgtcgatg atcttttctg cgccgatttc gtaagcatcc
    14401 tgatacagcg ccagattctc gttagtcata aacggggcag ggtagtaaac gcgtcctgtt
    14461 ttgccctctt tgcgtatctc tactttcgcc acaatcggat gaatactcga cgtagcatgg
    14521 ttgatataag agatagaacc ggttggcggc accgcctgga gattctggtt gtatatgccg
    14581 tagcgcatca cgtcgtcgcg cagttgcgcc cacatctcac gggtaggtaa cgtaataccg
    14641 ctgcgggcaa acagttcgcc aactttcgcc gttttcggct gccagttacc ttgcagatat
    14701 tggctgaaat attcaccact ggcatagcgt gactgtttga acccggcgaa ggtttcaccg
    14761 cgttcgcgcg ccagcaacat cgaggtacgc agtgcgtgcc aggtgatggt atagaaatag
    14821 agattggtga aatccagggc ttccggcgaa ccataagcga tgccttccct cgccagataa
    14881 ccatgtaaat tcatctgccc cagtccgatg gcgtgcgagg cggcatttcc ggcttcgatg
    14941 gacggcacgc tgcggatatg actcatatct gacaccgccg ttaaaccgcg tacggcagtc
    15001 tctaccgtgc gggcaaagtt gggggaatcc atggtgtggg caatattcaa cgaacctaaa
    15061 ttgcaggaaa tatcatggcc tgtacgggca tagtcgagat tctcgtcata ctctgaggcg
    15121 ctgttaacct gcaaaatttc tgagcagaga ttactcatat ttatgcgccc ggcgataggg
    15181 ttagcacggt ttaccgtgtc ttcatacatg atgtagggat agccggactc aaactggatt
    15241 tctgccagtc gctggaagaa atcacgggcg ttgaggtatt ttttgcggat gcgttcatcg
    15301 gcaaccagtt cgtcatagtg ttcgctaatg gcgatatcgg caaacggctt gccataaact
    15361 cgctcaacgt catagggcga aaacagcgcc atctgcgcat tctcttttgc cagatggaaa
    15421 gtgatatccg ggatcaccac gccaagcgac agtgttttaa tgcggatttt ttcgtcggca
    15481 ttttcccgtt tcgtgtcgag aaaacgcaga atatcgggat gatgagcatg taaatagact
    15541 gcaccagccc cctgacgcgc gccgagttgg ttgggatagg aaaatgcgtc ttccagcatt
    15601 ttcatcaccg gaattacgcc agaagattga ttttcaatac gtttaattgg cgcgcccgct
    15661 tcccgcagat tcgacagcaa aaatgccacg ccgccgccgc gtttcgacag ttgcagtgcg
    15721 gaatttaccg cccggccaat cgactccata ttgtcttcaa tacgcagcag aaaacaggaa
    15781 accagttcgc cgcgctgctg cttaccgcag ttgaggaatg ttggcgtggc tggctgaaag
    15841 cgtcctgaca gcatttcatc ggtcagttgc aacgccagcg tcgcatcgcc ttgtgccagc
    15901 gtcagcgcca ccatcgtgac tcgatcggca aaatcttcca gataacgttt accgtcgaat
    15961 gtcttcaacg tatagctggt gtaaaacttc catgccccga ggaatgtctg gaaacgaaaa
    16021 ccgctggtgt gtgcgtgggc aaacagcgta atgacaaagt cgcgagagta gcgattaaga
    16081 acgctttcat catagtaacc ttcgttgacc agccagttca ggcgctgctg ctggctactg
    16141 aaggtcacac tgttcggacg cacatgcgtc gcaataaagg cgtcaacggc ctggcggtct
    16201 ttatcgaact gaatgcgacc tgcgctatcg tagaggttaa gcatcgcatt cagcgcgtgg
    16261 taatccatcg tttcctgcgt caggcattct gcggttgtcg ttgccaaaat tcggttactc
    16321 ctttacgaac gttttcgata tcgctttgcg tacccatgag ttcaaaacgg tacagccacg
    16381 gcacgccgca tttccgggca atcacatctc cggcgcggcc atacgcctca ccaaagttgc
    16441 gattaccaga agcaataacg ccgcgaagca acgcccggtt gtgctcgtcg tttaaaaagc
    16501 gaattacctg tcgtggcacc gcgccagccg taccgccgcc gccgtaagag ggcacgatca
    16561 ggatgtaagg ctcgtctacc tgaatccgtt cccgctcatt gagcgggatg cgcaccgcgg
    16621 gcagacctaa acgttcgata aaacgctgcg tgttttcgga gctgctggag aagtagacga
    16681 gctggctcat gcactggccg cgtgtggcgc tggatgcaga cggttaatca tgtccggacg
    16741 gaaaccagac cagctaagat cgccagcaat cactaccggc aactgacgaa agccctgagc
    16801 acgcaacgct tccgctgctt caggaacgcg atcgacatta atcatttcaa aatcaaagcc
    16861 ccggttttcc atcgcccgtt tggtggcgtg gcactgaacg caatcgttac gagtgtaaat
    16921 agtaatgcgc atggttcgta tttccgttta aaatgaagat acggcgcgat gatacgcgtc
    16981 gggttgtctc tctgttgata cagagatact agatgtagtt gaaaaaagat tcaaccacac
    17041 aatatatagc aaatagtggt cgaaattacc ctggatatga gcgtgacggg gtagggggat
    17101 ttttgtgatt caccaggcaa aaagaaaccc cgaagccagg cttcggggtc aaagacgcgt
    17161 atttattatc atttttgcac tacgatttgc gcatgcttaa cagtgcgccg ataaaaatac
    17221 ctaccgcagc tgctgtaccc acgctacacc agggtctttc acgaacaaaa gaatccgcgc
    17281 agccaacggc atcgcgtgcg gcttgctgga cgcgagtacg accatgcatt cgcgccctgg
    17341 tttctttcag caatgcctga gctttactgc gtgcggcttc agcttcccct ttggcgtcgc
    17401 tgccccagga cttcaataca gattcgaggc tgtcagctaa ttgattgaca tcattctgga
    17461 tatcctgcac gccatcatca acgtcgttgc ggttcggtcg gttaaacata tgatccccca
    17521 ttagaattcg atgtaatttt cagtgtagac cataattttc ttaactgagg ttcatcgcgg
    17581 ctttatggac ttttctgaaa gcgttgtgaa tgcgcaatca ggtaaggtag gcccgcggtt
    17641 gataataacg aggatcaaat atgtatttac gaccagacga ggtggcgcgc gtacttgaaa
    17701 aagtcggttt tactgtcgat gtggtaaccc agaaagcgta tggttaccgc cgtggggaaa
    17761 attatgtcta tgttaatcgc gaagcgcgaa tgggacgtac cgctctggta attcatccaa
    17821 cattaaaaga acgcagttca acactggctg aacccgcttc cgatattaaa acttgcgatc
    17881 attaccagca atttccgctc tatttagcag gcgagcgaca cgagcattac ggtatcccgc
    17941 atggctttag ttcgcgtgtt gcgcttgaac gttatttgaa tggtttattt ggcgaagcca
    18001 gttaaagacg cgactggcga tgccagtcgc gaaaagaaga gtcaggcttt tacctgctgg
    18061 taacggctga ctttaaacag tcggcggcaa taatcgagga agtagccata aaccgccccc
    18121 atcaacatcg aaacaacgat gtttgaactg accgccgcca taatctgatg ccagtctgcg
    18181 cccaccacta acaagatcgc cacatacacc ggtgactgga acgtcacata agccaggata
    18241 tcagccagat tttttatcca gcccgacggg ctaactttgc gtgccgcgcg cataaacaga
    18301 tcgcggtaca taccgtatgg ccatgcaatt aagatgttcg ccggaatcgc taccaatctg
    18361 gaataaaaag actgttcgaa gctcattccg gagaggaaaa cttcaataca catgttcacg
    18421 acagaacagt aaacaaccat cgcgaacgta tctgcaactg catgacgcaa gcgtgactgc
    18481 ggtgagaaca tgctaatgct ccttaataaa agcgtaatgg gatgttaatg gagatgaaag
    18541 cagtggatct cattgccatc tgcattttcg catagcgtat atcgctggct atgactattc
    18601 aattagtgaa aaatttttat ttagtcagtt tttgtagata aaatattccg taacggctcg
    18661 ttttttgtat actgtggatt tttacatgcc gatatgattt attttcaatt aaatcaattg
    18721 gatatgacct tgcctttaat ttcactaatg aaagtttggg ggtgaggtaa cgctataagc
    18781 gtagagaatt cggtgtatgt cgtgaccggg tcatttacag cattttttat gccagaaaat
    18841 gtacattaac agcagaaagc accagtgagg ggtgttttct tttattctgt tatattgcat
    18901 ttttattcac ttcggt
//
LOCUS       DAZGJW010000087        18839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZGJW010000087 DAZGJW010000000
VERSION     DAZGJW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.087"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            180..908
                     /locus_tag="ACHKIK_003094"
     CDS             180..908
                     /locus_tag="ACHKIK_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO9084217.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKKINAVSTLITRNSEHH"
     gene            908..5758
                     /gene="ehaG"
                     /locus_tag="ACHKIK_003095"
     CDS             908..5758
                     /gene="ehaG"
                     /locus_tag="ACHKIK_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFO9084218.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKTTNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITASTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TSGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6127..7782
                     /gene="lldP"
                     /locus_tag="ACHKIK_003096"
     CDS             6127..7782
                     /gene="lldP"
                     /locus_tag="ACHKIK_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO9084219.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7782..8558
                     /gene="lldR"
                     /locus_tag="ACHKIK_003097"
     CDS             7782..8558
                     /gene="lldR"
                     /locus_tag="ACHKIK_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO9084220.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8555..9745
                     /gene="lldD"
                     /locus_tag="ACHKIK_003098"
     CDS             8555..9745
                     /gene="lldD"
                     /locus_tag="ACHKIK_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO9084221.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9931..10404
                     /gene="trmL"
                     /locus_tag="ACHKIK_003099"
     CDS             9931..10404
                     /gene="trmL"
                     /locus_tag="ACHKIK_003099"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO9084222.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10457..11278)
                     /gene="cysE"
                     /locus_tag="ACHKIK_003100"
     CDS             complement(10457..11278)
                     /gene="cysE"
                     /locus_tag="ACHKIK_003100"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO9084223.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11358..12377)
                     /gene="gpsA"
                     /locus_tag="ACHKIK_003101"
     CDS             complement(11358..12377)
                     /gene="gpsA"
                     /locus_tag="ACHKIK_003101"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO9084224.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12377..12844)
                     /gene="secB"
                     /locus_tag="ACHKIK_003102"
     CDS             complement(12377..12844)
                     /gene="secB"
                     /locus_tag="ACHKIK_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO9084225.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12907..13158)
                     /gene="grxC"
                     /locus_tag="ACHKIK_003103"
     CDS             complement(12907..13158)
                     /gene="grxC"
                     /locus_tag="ACHKIK_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO9084226.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13300..13731)
                     /locus_tag="ACHKIK_003104"
     CDS             complement(13300..13731)
                     /locus_tag="ACHKIK_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO9084227.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13976..15520
                     /gene="gpmM"
                     /locus_tag="ACHKIK_003105"
                     /gene_synonym="pgmI"
     CDS             13976..15520
                     /gene="gpmM"
                     /locus_tag="ACHKIK_003105"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO9084228.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15554..16813
                     /gene="envC"
                     /locus_tag="ACHKIK_003106"
     CDS             15554..16813
                     /gene="envC"
                     /locus_tag="ACHKIK_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO9084229.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16817..17776
                     /locus_tag="ACHKIK_003107"
     CDS             16817..17776
                     /locus_tag="ACHKIK_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO9084230.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMRALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17763..18797)
                     /gene="waaH"
                     /locus_tag="ACHKIK_003108"
     CDS             complement(17763..18797)
                     /gene="waaH"
                     /locus_tag="ACHKIK_003108"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO9084231.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
BASE COUNT         4754 a         4860 c         4873 g         4352 t
ORIGIN      
        1 tattcataca aacacagaca tcttttgaaa cgaatgatac atttttatga tgaatgctta
       61 aaacaatatt taatcgacac acatcactgt cattaatgta aagcaacata gatcaccaat
      121 aatacaatgg acaactctac cggcattgat cgctggtcgg aggaaataag gatttttaca
      181 tgaacttgaa gaagattttt ttcagtgctg tcactgtttc agttctctgc gcattaacgg
      241 gatgtgacta cattgaagaa ggcaagccag aaagtagttt attgaaacaa caagaagaac
      301 ataacaataa aattgatcta cttgaaaaac aacaagcaca gctaaagagt caactggaaa
      361 ctattcaaaa acaacaaact ggcataataa gcagtacaaa gactttaact cacgtaatta
      421 aatccgttaa agatcaacaa aacactttta tttttaccga gtttaaccca gcaaaaacga
      481 agtatttcat cctgaacaat ggttcggtgg ctttagcggg tcgagtgtta tccattgacg
      541 ccacagagaa tggcagtgtt attcatattt cactggtcaa cttattaagt acacctatct
      601 caaatattgg ttttaatgcg acatggggtg gtgaaaaacc tgtcgatgcc aaagagtttg
      661 cccggtggca acaattactt ttcaacacat caatgaaatc cacattgaaa ttattaccag
      721 gtcaatggca agacattaat ttgaccctga agggtgtatc gcccaataac ctgggatatc
      781 tgaagttagc catcaacatg gaaaatattc agttcgacaa tcttccttct gctgaaaatc
      841 ggcagaaaag aagcaaaaaa ataaatgcag ttagtacgct tataacaagg aatagcgagc
      901 atcattaatg aacaaaatat ttaaagttat ctggaaccct gcgacaggga attatactgt
      961 taccagcgaa acggcaaaaa gccgtggcaa gaaatctggg cgcagtaagc tgttaatttc
     1021 tgcgctggtt gcgggtggaa tgttgtcgtc gtttggggca ttggcgaatg ccgggaatga
     1081 caacggtcag ggtgttgatt acggtagtgg atcagctggc gacggctggg ttgctatagg
     1141 caaaggggcg aaagcaaata cttttatgaa caccagtggt tccagtactg ctgtgggtta
     1201 tgacgctata gctgaaggcc aatatagctc tgccatcggg tcaaaaaccc atgcgattgg
     1261 tggtgcatca atggcctttg gggttagtgc aatatcagaa ggcgatagaa gtatagcact
     1321 gggtgcctct tcgtattcat tgggccaata ctcaatggcc ctcggccgtt attcaaaagc
     1381 attgggtaaa ttgtctattg ctatggggga ctcttccaaa gcggaaggag caaacgccat
     1441 tgccctggga aatgccacta aagctactga gattatgagt attgctcttg gcgacaccgc
     1501 caatgcgtca aaagcgtatt caatggcgct gggagcaagt agcgtcgcat ctgaagaaaa
     1561 cgcaattgcc ctggggcgta gcagtgtagc tagcggtact gacagcctcg catttggcag
     1621 acaatcactt gccagcgcag cgaacgctat tgcgataggt gctgagaccg aagccgctga
     1681 aaatgcaact gctattggca ataatgcgaa ggcaaaaggg actaatagca tggcaatggg
     1741 gttcggaagc cttgccgata aagtcaatac tatcgcatta ggaaatggca gccaggctct
     1801 ggcagataat gcaatcgcca taggccaggg caacaaagct gatggcgtgg atgccatcgc
     1861 tctgggtaat ggtagccagt cgagaggctt aaacaccatt gccttaggca cagccagtaa
     1921 tgcaactggt gataagagtc ttgcgcttgg tagtaatagc agtgccaacg gtattaactc
     1981 tgtcgcgctg ggcgcagatt ccattgcgga tttagacaat accgtctctg tcggcaatag
     2041 ttcattaaaa cgcaagatcg ttaatgtgaa aaatggcgcg atcaagtctg acagttacga
     2101 tgccattaat ggttcacagc tttatgccat tagcgactcg gtagcaaaaa ggcttggagg
     2161 aggggctgca gtagatgttg atgacggtac tgttacagca ccaacctaca atttaaaaaa
     2221 tggtagcaaa aataacgtag gggctgcgct cgctgtactt gatgaaaaca ccctgcaatg
     2281 ggaccaaacc aaaggcaaat acagcgctgc tcatggtact agtagcccaa ctgccagcgt
     2341 aatcaccgat gttgcggatg gcacgatttc agcctccagt aaggatgcgg ttaacggttc
     2401 ccaactgaaa actaccaatg acgatgtcga agccaacacc gccaatatcg ctactaatac
     2461 cagcaacatt gccacgaata cggcaaatat tgccaccaat accaccaata tcaccaacct
     2521 gacggattcc gttggtgacc ttcaggctga tgccctgctc tggaacgaaa ctaaaaaggc
     2581 attcagtgca gctcacggcc aggataccac cagcaaaatc accaacgtta aagatgccga
     2641 cctgacggct gacagcactg atgctgttaa cggctctcag ctgaaaacca ccaacgatgc
     2701 tgtggcgacg aataccacca atatcgccaa taacacttcc aatattgcca ctaacaccac
     2761 caacatctct aacctgactg agacggtgac taatcttggt gaggatgcgc tgaaatggga
     2821 taaggacaat ggtgtattca cggcagctca tggcaccgag accaccagca aaatcaccaa
     2881 cgttaaagat ggcgacctga cgactggcag caccgatgcc gttaacggct ctcagctgaa
     2941 aaccaccaac gatgccgtgg cgacgaatac caccaatatc gccactaaca ccaccaacat
     3001 ctctaatctg actgagacgg tgactaatct tggtgaggat gcgctgaaat gggataagga
     3061 caatggtgtc ttcactgcag ctcatggcaa caataccgcc agcaaaatca ccaatatcct
     3121 ggacggcaca gtcactgcaa ccagttccga tgccattaac ggtagccagc tttatgactt
     3181 aagcagcaat atcgccacct acttcggcgg caatgcttct gtgaatactg acggtgtgtt
     3241 taccggtcca acctacaaaa tcggtgaaac aaattattat aacgtcggcg atgcactggc
     3301 tgcgattaac tcctcattta gcacgtctct cggcgatgct ctgctttggg atgccaccgc
     3361 aggtaaattc agtgccaaac acggtactaa tggtgacgca agcgtgatca ctgatgtcgc
     3421 agatggtgaa atttcagact ccagttctga cgcagtaaac ggctcacaac tccacggcgt
     3481 gagcagttat gttgttgatg cgctgggggg tggtgccgaa gtcaatgcag acggcaccat
     3541 cactgcgccg acgtacacca ttgctaatgc tgattacgat aatgtcggtg atgccctgaa
     3601 tgctatcgat accactcttg acgacgctct gctctgggat gcggacgccg gtgaaaatgg
     3661 tgcatttagc gccgctcacg gaaaagataa aactgccagt gtaatcacta acgtcgctaa
     3721 cggtgcaatc tctgctgcca gcagcgacgc gattaacggc tcacaactct ataccaccaa
     3781 taagtacatc gctgatgcgc tgggtggtga cgcagaagtc aacgctgacg gcaccatcac
     3841 cgcatcgact tacaccattg cgaacgccga gtacaacaac gtcggtgacg ccctggatgc
     3901 gcttgatgat aacgccttgc tgtgggatga gactgccaat ggcggtgctg gagcctacaa
     3961 tgccagccat gacggtaaag ccagcatcat cactaatgtc gctaatggca gtattagtga
     4021 ggacagtacc gatgcagtga acggttctca gttgaatgcg acgaatatga tgattgagca
     4081 gaacacccaa attatcaatc agctcgctgg taacaccgac gcaacctata tccaagaaaa
     4141 cggtgcgggt attaactatg tgcgtactaa cgacgacggc ttagcgttca acgacgccag
     4201 cgcacagggt gttggcgcta cagctatagg ttataactct gtcgccaaag gcgatagcag
     4261 cgtagctatt ggtcagggca gctacagcga cgttgatacg ggtatcgccc tgggtagcag
     4321 ctctgtttcc agccgagtga ttgccaaagg ctcccgtgac accagcataa cggaaaatgg
     4381 cgttgttatt ggttacgaca ccacggatgg cgaactgctc ggtgcattgt ctatcggtga
     4441 tgacggtaaa tatcgtcaaa tcatcaacgt agccgatggt tccgaagccc atgacgccgt
     4501 tacggttcgt caattgcaga atgcgattgg tgcggtcgca accacgccga ctaaatactt
     4561 ccacgctaat tcaacggaag aagattcact ggcagtggga actgactcgc tggcaatggg
     4621 tgcgaaaacc atcgtgaatg gcgataaagg tattggtatc ggttatggtg cctacgtgga
     4681 cgcgaatgca cttaacggca ttgccattgg tagcaatgcg caagtcattc atgtcaacag
     4741 tattgcgata ggtaatggtt ctacgaccac tcgtggcgct caaaccaatt ataccgccta
     4801 caacatggac gcaccgcaga actctgtcgg tgaattctca gtcggtagtg cggatggtca
     4861 acgtcagatc actaacgtcg cagcaggttc ggctgatacc gatgcggtca acgtgggtca
     4921 gttgaaagta acggatgcgc aggtttccca gaatacccag agcattacta acctgaacaa
     4981 tcaggtcact aatctggata ctcgcgttac taatatcgaa aacggtattg gcgacattgt
     5041 cacctccggt agcaccaagt acttcaagac caacaccgat ggcgtagatg ccaacgccca
     5101 gggtaaagat agcgttgcta ttggttctgg ttccattgct gccgctgaca acagcgtcgc
     5161 actgggtacc ggttccgttg cagaggaaga aaatacaatc tctgtaggtt cttctactaa
     5221 ccaacgccgt atcaccaacg tagctgccgg tgttaatgcc accgatgcgg ttaacgtgtc
     5281 gcagctgaaa tcttctgaag caggcggcgt gcgctacgac accaaagctg atggttctat
     5341 cgactatagc aatatcaccc tcggtggtgg caacggcagt acgactcgta tcagcaacgt
     5401 ctccgctggc gtcaacaaca acgacgcggt gaactacgcg cagttgaagc aaagcgtgca
     5461 ggaaacgaag caatacaccg atcagcggat ggttgagatg gataacaaac tgtctaaaac
     5521 tgaaagcaag ttgagcggtg gtatcgcttc tgcaatggca atgaccggtc tgccgcaggc
     5581 ttatacaccg ggtgccagca tggcttctat tggtggcggt acttacaacg gtgaatcggc
     5641 agttgcttta ggtgtatcga tggtgagcgc caatggtcgt tgggtctaca aattacaagg
     5701 tagtaccaat agccagggtg aatactccgc cgcactcggt gccggtattc agtggtaatg
     5761 atccattaag ttagtgtgac taagggcaaa cgccaaattg cctgatgcgt tacgcgcatc
     5821 aggcaagata aggcgctccg ggtcaacaat ctaagggcaa ttctctgatg aggattgccc
     5881 ttttctttac cagacatctc ccccctacaa gaattggccc taccaattct tcgcttatct
     5941 gacctctggt tcacaattcc ccaattaaaa ctcacatcaa tattgccaat acataacatt
     6001 tagttaacca ttcattgtca ttatccctac acaacacaat tggcagtgcc acttttacac
     6061 aacgtgtgac aaggagatga gcaacagact cattacacga tgtgcgtgga ctccaggaga
     6121 cctgcaatga atctctggca acaaaactac gatcccgccg ggaatatctg gctttccagt
     6181 ctgatagcat cgcttcccat cctgtttttc ttctttgcgc tgattaagct caaactgaaa
     6241 ggatacgtcg ccgcctcatg gacggtagca atcgcccttg ccgtggcttt gctgttctat
     6301 aaaatgccgg tcgctaacgc gctggcctcg gtggtttatg gcttcttcta tggtttgtgg
     6361 cccatcgcgt ggatcattat tgcagcagtg ttcgtctata agatctcggt gaaaaccggg
     6421 cagtttgaca tcattcgctc gtctattctt tcgataaccc ctgaccagcg tctgcaaatg
     6481 ctgatcgtcg gtttctgttt cggcgctttc cttgaaggag ccgcaggctt tggcgcaccg
     6541 gtagcaatta ccgccgcatt gctggtcggc ctgggtttta aaccgctgta cgccgccgga
     6601 ctgtgcctga ttgttaacac cgcgccagtg gcatttggcg cgatgggcat tccaatcctg
     6661 gttgccggac aagtaacagg catcgacagt tttgagattg gccagatggt ggggcgtcag
     6721 ctaccgttta tgaccattat cgtgctgttc tggatcatgg cgattatgga cggctggcgc
     6781 ggtatcaaag agacgtggcc tgcggtcgtg gttgcgggcg gctcgtttgc catcgctcag
     6841 taccttagct ccaacttcat tgggccggag ctgccggaca ttatctcttc gctggtatca
     6901 ctgctctgcc tgacgctgtt cctcaaacgc tggcaaccag ttcgcgtatt ccgcttcggt
     6961 gacttggggg cgtcacaggt tgatatgacg ctggcccaca ccggttacac cgcgggtcag
     7021 gtgttacgtg cctggacacc gttcctgttc ctgaccgcta ccgtaacact gtggagcatc
     7081 ccgccgttta aagccctgtt cgcatcgggt ggtgcgctgt atgagtgggt gatcaacatt
     7141 ccggtgccgt acctcgataa actggttgcc cgtatgccac cagtggtcag cgaggctaca
     7201 gcctatgccg ccgtgtttaa gtttgactgg ttctctgcca ccggcaccgc cattctgttt
     7261 gctgcactgc tctcgattgt ctggctgaag atgaaaccgt ctgacgctat cagcaccttc
     7321 ggcagcacgc tgaaagaact ggctctaccc atctactcca tcggcatggt gctggcgttc
     7381 gcctttatct cgaactattc cggactgtca tcaacactgg cactggcact ggcgcacacc
     7441 ggtcatgcat tcaccttctt ctcgccgttc ctcggctggc tgggggtatt cctgaccggg
     7501 tcggatacct catctaacgc cctgttcgcc gcgctgcaag ccaccgcagc acaacaaatt
     7561 ggcgtctctg atctgttgct ggttgccgcc aataccaccg gtggcgtcac cggtaagatg
     7621 atctccccgc aatctatcgc tatcgcctgt gcggcggtag gcctggtggg caaagagtct
     7681 gatttgttcc gctttactgt caaacacagc ctgatcttca cctgtatggt gggcgtgatc
     7741 accacgcttc aggcttatgt cttaacgtgg atgattcctt aatgattgtt ttacccagac
     7801 gcctgtcaga cgaggttgcc gatcgtgtgc gggcgctgat tgatgaaaaa aacctggaag
     7861 cgggcatgaa gttgcccgct gagcgccaac tggcgatgca actcggcgta tcacgtaatt
     7921 cactacgcga ggcgctagca aaactggtga gcgaaggcgt gctgctcagt cgacgcggcg
     7981 gcgggacgtt tattcgctgg cgtcatgaca catggtcgga gcaaaacatc gtccagccgc
     8041 taaaaacgct gatggccgat gatccggatt acagtttcga tattctggaa gcccgctacg
     8101 ccattgaagc cagcaccgca tggcatgcgg caatgcgcgc cacacctggc gacaaagaaa
     8161 agattcagct ttgctttgaa gcaacgctaa gtgaagaccc ggatatcgcc tcacaagcgg
     8221 acgttcgttt tcatctggcg attgccgaag cctcacataa catcgtgctg ctgcaaacca
     8281 tgcgcggttt cttcgatgtc ctgcaatcct cagtgaagca tagccgtcag cggatgtatc
     8341 tggtgccacc ggttttttca caactgaccg aacaacatca ggctgtcatt gacgccattt
     8401 ttgctggtga tgccgacggg gcgcgtaaag caatgatggc gcaccttagt tttgttcaca
     8461 ccaccatgaa acgattcgat gaagatcagg ctcgccacgc acggattacc cgcctgcccg
     8521 gtgagcataa tgagcattcg agggagaaaa acgcatgatt atttccgcag ccagcgatta
     8581 tcgcgccgca gcgcaacgca ttctgccgcc gttcctgttc cactatatgg atggtggtgc
     8641 atattctgaa tacacgctgc gccgcaacgt ggaagatttg tcagaagtgg cactgcgcca
     8701 gcgtattctg aaaaacatgt ccgacttaag cctggaaacg acgctgttta atgagaaatt
     8761 gtcgatgccg gtagcactgg caccggtggg tttgtgtggc atgtatgcgc gtcgtggcga
     8821 agttcaggca gccaaagcgg cggacgcgca tggcattccg tttactctct cgacggtttc
     8881 cgtttgcccg attgaagaag tcgcgccagc catcaagcgc ccaatgtggt tccagcttta
     8941 tgtactgcgc gatcgcggct ttatgcgtaa cgcgctggag cgagcaaaag cagcgggttg
     9001 ttcgacgctg gttttcaccg tggatatgcc gacaccgggc gcacgctacc gtgatgcgca
     9061 ttcaggtatg agcggcccaa atgcggcaat gcgccgctac ttgcaggcgg tgacgcatcc
     9121 gcaatgggcg tgggatgtgg gcctgaacgg tcgtccacat gatttaggta atatctcagc
     9181 ttatctcggc aaaccgaccg gactggaaga ttacatcggc tggctgggga ataacttcga
     9241 tccgtccatc tcatggaaag accttgagtg gatccgcgat ttctgggatg gcccgatggt
     9301 gatcaaaggg atcctcgatc cggaagatgc gcgcgatgca gtacgttttg gtgctgatgg
     9361 aattgtggtt tctaaccacg gtggccgcca gctggacggt gtactctctt ccgctcgtgc
     9421 gttgcccgct attgcagatg cggtgaaagg tgatatcgcc attctggcgg atagcggaat
     9481 tcgtaacggg cttgatgtcg tgcgtatgat tgcgctcggt gccgacaccg tactgctggg
     9541 tcgtgctttc ctgtatgcgc tggcaacagc gggccaggcg ggtgtagcta acctgctaaa
     9601 tctgatcgaa aaagagatga aagtggcgat gacgctgact ggcgcgaaat cgatcagtga
     9661 aattacgcaa gattcgctgg tgcaggggct gggtaaagag ttgcctacgg cactggctcc
     9721 gatggcgaaa gggaatgcgg catagtcgtt tgcgccccct caccctaacc ctctccctca
     9781 gggagagggg accgttcggc gctgtatgta ctccctcact ctgaaacgac accgcactct
     9841 ttttttctcc ctcgcccctc cggggtgagg ggaaaaggcc gcactgctca ccaattttct
     9901 gctatcctgc ccccgcacta agggggcagt atgctaaaca tcgtacttta cgaaccagaa
     9961 attccgccaa atactggcaa tatcatccgt ctttgcgcta ataccggctt tcgtctgcat
    10021 atcatcgaac cgatgggatt tgcctgggac gataagcgcc tgcgccgcgc ggggctggac
    10081 tatcacgagt ttaccgccgt tacgcgtcat catgactttc gcgcgttcct cgaagcagaa
    10141 aatccccagc gcctgtttgc cctcactacg aaaggtacgc ctgctcacag cgccgtaaac
    10201 tatcaggatg gcgattatct gatgttcggc ccggaaacac gcggcctgcc agcgagcatt
    10261 cttgatgccc tgcccgctga acaaaaaatt cgcattccaa tggtgccgga cagccgcagc
    10321 atgaacctgt ccaatgcggt gtcggtagtg gtgtatgaag cctggcggca gctgggatat
    10381 ccgggagcgt tgttgagaga ttagttactg tatgccggat agatgattac atcgcatccg
    10441 gcacgatcac agcacatcag atcccatccc catactcaaa tgtatggtta ataccgttga
    10501 aatgctggtc catatccatt gatggcttat cgctgtctgg tttaccgaca atacgagccg
    10561 gaacaccagc ggcggtagta tgcggcggca ccggttgcag caccacggaa cctgcgccaa
    10621 tcttcgcgcc gcgaccaact tcaatattgc cgagaatttt cgcgcccgcg ccaatcatca
    10681 caccttcacg aattttcggg tgacggtcac caccagattt acccgtaccg ccaagcgtca
    10741 cagattgcag aatcgatacg tcgttttcaa ttaccgccgt ttcaccaacg acgatgcccg
    10801 tggcgtggtc gagcatgata ccgcgaccaa tttttgctgc cgggtgaata tcgacctgga
    10861 acgtcacaga aacctggttt tgcagaaaga ttgccagtgc gcgacgcccc tgattccaca
    10921 accagtgacc gatgcgatag gcctgcaagg catgaaaacc cttcaggtat aacaacgggg
    10981 ttgagtattt atcgactgcc gggtcgcggg tacgcaccgc ctgaatatca caggccgcag
    11041 aggcgatcat ttccgggtca gcggcgtagg cttcttcaac cacttcacgg atagcaatag
    11101 caggcataat tggcgatgac agcttgttcg ccagcatgta gctcagggca ctgccaaggt
    11161 tttcgtgctt gagtagcgtc gcgtggtaaa aactggccag cattggctca cagtccgcca
    11221 gcgttctggc ttcggcttta atattgttcc agacaatttc cagttcttca cacgacattg
    11281 cttactccac acgatgagat aatgaccggc ccgttctgcg cgggccgggt catagcggta
    11341 acaaaggttc cctggggtta gtggctgctg cgctcgtcct tgcgtgcacg acctagtaaa
    11401 gtcaatgctg cctcgcgcgc gttttttccg caatataata cttgataaat ttcctcggtt
    11461 attggcattt caacgccgaa gcgatgcgcc agttcgcgga cttctttcgt attgcggtag
    11521 ccttccacca cctgaccaat cttctcctgc gcgctttgta catccatgcc ctgaccgagc
    11581 atcatgccaa aacggcggtt acgcgactgg ttgtcggtac aggtaagcac cagatcgccc
    11641 agccccgcca tgcccataaa ggtggcagga tcggcaccca gcgccgcacc aagacgcgac
    11701 atttcagcca gcccacgggt gatcagcgcc gtacgcgcat tcgcaccaaa accgataccg
    11761 tcagacatcc ccgcgccaat ggcaatgacg tttttcaccg cgccgccaag ctgcacgcca
    11821 atgaaatccg ggttgctgta aacgcggaaa cttttgccac agtgcagcaa ttgttggaga
    11881 tcatcggcaa aggtctggtc ggtagaggcc aacgaaatag ccgtcggtaa acctgccgcc
    11941 agttctttcg caaacgttgg gccagagata accgccagcg gaatttgatc gcctaaggcc
    12001 tcacgcgcca cgtcctgtaa cagacgtccg gtttccgctt ccagcccttt ggtcgcccac
    12061 accagacgcg catcaggacg catcagcggt ttaatctggc gcagcacttc accaaagaca
    12121 tggctgggta cgacgacgag aatattacgg ctggctgcca gcgcagtggc gagatcgctt
    12181 tcaagatgga gcgtatcggg aaaaggcaca tcggggagaa acgcggcgtt acagcggtcg
    12241 cgttcaagcg ttgcgatatg ttcagggtca tggccccaga ggacaacctc gtggccattt
    12301 cttgccaggg taatggcaag agcggtgccg tacgagccgg caccgatcac agtcattgaa
    12361 gcattacgtt ggttcatcag gcatcctgat gttcttcagt accttcgcca gcctgctgct
    12421 gcaaatagtt catgaacagc gcatcgaagt taaccggcgc aaggttcagt tgcgggaatg
    12481 taccgcggga taccatgctg gtgatgcact cacgagcata cgggaacaga atgttcgggc
    12541 agtatgcgcc caggcaatgt gccatctggg tgccttcgat acccgcgatg gagaaaatac
    12601 cgccctgctg aacttcacac aggaacgcgg tttcttcgcc cagggaggcc gttacggtaa
    12661 cacgcagtac cacttcgtat acgtcatctg ccagttggga agatgccgta tccagatcaa
    12721 gtttaacttc tggttgccaa tctttctgga aaacgtgcgg cgcgttcggc gcttcgaaag
    12781 agatatcctt ggtataaata cgttggatct ggaaagtcat ttcagtgttg ttttgttctg
    12841 acatgtgtag aaaaccctta agtgttgtcc ttaaatactg cgcaatgtaa tgccaacgtt
    12901 cacacgttat ttcagcaggg gatccagtcc accacgtgca tccaatgcat acaagtcatc
    12961 acagccgcca atgtgctgtg cgtcaataaa aatctgggga accgtggtgc gaccgctgcg
    13021 tttgatcatc tcttcacgct tggcggcatt gccatcgatc ggcaactcct gaaaactcac
    13081 gcccttgctg ctcagcagtg cttttgcacg atggcaatac gggcaggttt ctttggtata
    13141 gatttcaaca ttggccataa tttagctcct gtttttttac cctgaaattt catgttgcag
    13201 ggcggctgca aaatggagtg tccccagaag cttataacaa taagtcgctg ggggcctaat
    13261 tgcagctaac ggcctgcatc atgaaagacg acaggtaaat tatttgccgc gcaccagagg
    13321 caagttttcg ccagcccagc cagcaacgcc ctctttcagc acgaatactt gcgcaaaacc
    13381 agctttcgtc agcgcgtttg caggctcctg gcactgcatg ccagaaccgt ctaccacgat
    13441 aaccggtttg tctttgtgct tctcaagctc accaacattg ttggctttga tttcactcgg
    13501 caacagattg atagaacctg cgatatggcc tttacggaag tcatcacgct gacgtaaatc
    13561 cacaaccaca gcgtcttctt tgttgatcag acgcgtagct tcaccacgag taatcacctt
    13621 cactttcgag gtcaggctct taaacgtagt cacaagaacc gccaccagta acgcgatcca
    13681 ggcgatactc agtatgggat gacggccaac aaattgcata atttcttgca tggggggtaa
    13741 caactcccga cgtagtgatt aaaaaaacca ggaaaggagt atacctgcgc ggtgcggcaa
    13801 atacagccag cgcgttaact ggaatgcaat tttgcggggc acgacgaaaa aaaagcgcaa
    13861 atctggcatc ggcttgccgc gagcggtcgt atattttgat cttcagaggc tattttatcg
    13921 attcagctgt agtaaaatta cgcaaatttt gactcttgag tatgaggttg tcgcaatgtc
    13981 ggtttctaaa aaacctatgg tactggtgat tctggatggc tatggctatc gtgaagaaca
    14041 gcaggataac gccattttta gtgctaaaac cccggtaatg gatgcactgt gggccaatcg
    14101 tccgcatacc ctgatcgacg cttccggtct ggaagtcggt ctgcctgacc gtcagatggg
    14161 taactccgaa gtaggccacg ttaacctggg tgccggccgc atcgtgtatc aggacctgac
    14221 tcgtctggac gttgaaatca aagatcgcgc tttctttgct aatccagtgc tgactggcgc
    14281 ggtagataaa gcgaaaaacg caggtaaagc agtacacatt atgggtctgc tctccgcagg
    14341 cggcgtacac agccacgaag atcacatcat ggcgatggta gaactggcag ctgaacgcgg
    14401 cgcagaaaaa atctacctgc acgcattcct tgacggtcgc gacaccccgc cgcgcagtgc
    14461 tgaatcctcg ctgaaaaaat ttgaagagaa atttgctgcg ctgggtaaag gccgcgtagc
    14521 gtccatcatt ggtcgttact acgcgatgga ccgcgataac cgttgggatc gcgttgaaaa
    14581 agcctatgac ctgctgactc tggcgcaggg tgagttccag gccgatactg ccgttgctgg
    14641 tttgcaggca gcttatgctc gcgacgaaaa cgacgagttc gtgaaagcga ccgttattcg
    14701 tgcagaaggt cagccagatg cggcaatgga agacggcgat gcgctgattt tcatgaactt
    14761 ccgtgctgac cgcgcgcgtg aaatcactcg tgcattcgtg aacgctgatt tcgatggctt
    14821 cgcgcgtaag aaagtggtta acgtcgattt cgtgatgctg accgaatacg ccgctgacat
    14881 caaaactgcg gttgcttacc cacccgcttc cctggttaac accttcggcg agtggatggc
    14941 gaaaaacgac aaaactcagt tgcgtatttc cgaaaccgaa aaatatgccc acgttacttt
    15001 cttcttcaac ggtggcgtag aagagtcgtt caaaggcgaa gatcgcattc tgatcaactc
    15061 accgaaagtg gctacctacg atctgcaacc ggaaatgagc tccgcagagc tgaccgaaaa
    15121 actggttgcg gccatcaaga gcggcaaata cgacaccatt atctgtaact atccgaacgg
    15181 cgacatggta ggtcacaccg gggtgatgga agcggcggtt aaagcggttg aagcactgga
    15241 tcactgcgtg gaagaagtcg cgaaagcggt tgagtccgtg ggtggacaac tgctgatcac
    15301 cgctgaccac ggtaacgctg aacagatgcg cgatccggca acgggtcagg cacacacggc
    15361 acacaccaac ctgccagttc cgctgattta tgttggtgat aagaacgtga aagcggttga
    15421 aggcggcaaa ctttctgaca tcgcgccgac catgttgtcg ctgatgggta tggaaatccc
    15481 gcaagagatg actggtaagc cgctgttcat cgtggaataa tccctcccca tgaggggaaa
    15541 ggcgattaat accatgacac gggccgtgaa accgcgcagg tttgcaatca ggcccatcat
    15601 ctacgccagc gttcttagcg ctggcgtatt gttgtgcgcc ttttccgccc acgcggatga
    15661 gcgtgaccaa ctcaaatcta ttcaggccga tatcgccgca aaagagcgcg cggtacgcca
    15721 aaagcaacaa caacgcgcaa gcctgctcgc acaattgaaa aagcaggaag aagcgatctc
    15781 tgaagccacc cgtaagctgc gcgaaacgca aaacacgctc aatcaactca ataaacagat
    15841 tgatgagatg aacgcgtcga ttgccaaact ggagcagcaa aaagccgccc aggagcgcag
    15901 cctcgccgcg caactggatg ccgcattccg tcagggcgag cataccggta ttcagctaat
    15961 tctcagcggt gaagaaagcc agcgtggaca gcgtttacag gcttatttcg gctatctcaa
    16021 ccaggcgcga caagaaacca ttgcccagtt gaagcaaacg cgtgaagaag tcgccatgca
    16081 gcgtgctgaa ctggaagaga aacaaagcga gcaacaaacg ctgttatatg aacagcgcgc
    16141 ccaacaggcg aaactgactc aggctctgaa cgagcgtaaa aagacgctgg cggggctgga
    16201 atcttccatc cagcaaggtc agcagcagct aagtgaactg cgtgccaacg aatcccgtct
    16261 gcgtaacagc attgcccgtg cggaagccgc ggcgaaagcg cgtgctgaac gagaagcgcg
    16321 tgaggcccag gcggttcgcg accgccagaa agaagcgacg cgcaaaggca ccacctataa
    16381 accgaccgaa agcgaaaaat cgctgatgtc ccgaactggt ggcctgggtg cgccgcgtgg
    16441 tcaggcattc tggccggttc gcgggccgac gctgcatcgc tatggtgaac agctacaggg
    16501 cgaactacgc tggaaaggaa tggttatcgg tgcctctgaa ggtactgaag ttaaagcgat
    16561 tgccgacggt cgggtgattc tggctgactg gctgcaaggt tacggtctgg tggtggtggt
    16621 tgagcacggt aaaggcgaca tgagtcttta cggctataat cagagcgcac tggtgagcgt
    16681 tggttcgcag gttcgcgcgg gccagccaat tgcactggtg ggcagcagtg gcggtcaggg
    16741 tcggccttca ctctatttcg aaattcgccg ccagggtcag gcggtcaatc cacagccgtg
    16801 gttgggaaga taagttttgt ttccatttcg tcgtaacgtt cttgcatttg ccgctctgtt
    16861 ggcgctctcc tcccccgtac ttgctggcaa acttgccatc gtcattgatg attttgggta
    16921 tcgcccgcac aacgaaaacc aggtgctggc gatgccctcc gctatctccg tcgctgtatt
    16981 acccgattca ccgcacgcca gagagatggc gaccaaagcg cataacagcg ggcacgaagt
    17041 gttgattcat ctcccgatgg caccactgag taaacagccg ctggagaaaa atacgctgcg
    17101 cccggagatg agcagcgacg aaattgagcg cattattcgt agtgcggtca ataacgtgcc
    17161 ctatgccgtg gggatcaaca accacatggg tagcaagatg acctctaacc tgtttggtat
    17221 gcagaaagtg atgcgggcac tggagcgtta caatctttac ttcctcgaca gcgtaaccat
    17281 cggtaatacc caggcgatgc gcgccgcgca aggtactggc gtgaaggtga tcaaacggaa
    17341 ggtgttcctc gacgattcgc agaacgaagc ggacatccgt gtgcaattta atcgcgcaat
    17401 tgatctggcg cgtcgcaacg gttcaaccat tgccattggg catcctcacc cttcaacggt
    17461 acgcgtgcta caacagatgg tttataacct gccgccagac attacgctgg tgaaagccag
    17521 cagcttgctg aatgaaccgc aggttgatac ttctacaccg ccgaaaaacg ctgtgcctga
    17581 tgcaccgcgt aatccgttcc gtggcgtgaa gctgtgcaaa ccgaagaaac cgatagaacc
    17641 tgtttatgct aatcgcttct ttgaagtatt aagcgaaagc atcagccaga gcacgctgat
    17701 cgtttacttc cagcatcagt ggcaaggctg gggcaaacag cccgaagcgg cgaagtttaa
    17761 cgctagcgca aattaaaggc gctacgcgta atgcgatggt gcgaccgcgt tttgtcgcgc
    17821 cattgccata agcggaaaga ccacagtaac gcctggtaac cgaccttcac gctgcgcaca
    17881 ttggtaatca ggcgcttata cataccggaa gtaaatatct cggcaatcat ccgttggcgg
    17941 gtaagaatat ccggctcttt gcgcaccgca tggcaaacac gcaatgcttc gtaagttatt
    18001 tgctgatgaa attccggata aatcgtaatt ttgtcggcat aatttcgatt taatttctcc
    18061 agcaggcggg taatcttaat atagtgacgt tgataattaa ggtttttatt cccttgtcta
    18121 tgcaaccgac tcactgacgt attatgcaga taatatttat ataatgattg ctcggtatat
    18181 cgcgcacgca gcgcgttaaa catgaattct gttgtccaga caatatcctg atgatgtaat
    18241 ccggcaataa atttaatgtt atttttaaca ataacatcac ggcgataaac ccccatccag
    18301 acaacgtgag tccaacggcg cgaagaaagc cccatccgca gccagtccgg gccggttaat
    18361 acgccggttg agcgaaggcg atcgctgggg atggattgcc aggtttctcc cgtttcacga
    18421 aaacaccagt cagcgttgca ctgcgccacg tcgaggtcat cctctaacgc catggtcatc
    18481 agcgtttcgt acatggtggg atagacctca tcgtcagcat cgacaaaagc gacatatttg
    18541 cccgtcgcca cctcgatccc acgattacgc gctaccgatg cgccagcatt cgcctgatgc
    18601 aacaaacgaa cgtgcggata gttttctgcg taatgctttg ctatttcaac agaattatcc
    18661 gttgaaccat cgttaataat aatgatttcc agagcagtcc aggtttgcgt aattaaagat
    18721 tccatacaag tgcggaaatc atcgcccgca ttatataacg gaataataac actaagttta
    18781 ttggtgctgt tcatcataaa ttaccgacga cctctgaagt tatcctgacg ttttacagg
//
LOCUS       DAZGJW010000088        18455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZGJW010000088 DAZGJW010000000
VERSION     DAZGJW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.088"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1546
                     /gene="mltF"
                     /locus_tag="ACHKIK_003109"
     CDS             <1..1546
                     /gene="mltF"
                     /locus_tag="ACHKIK_003109"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO9084232.1"
                     /translation="KINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGELRVS
                     TIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNADLLA
                     AGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVVVNDL
                     QTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALD
                     ITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTF
                     LRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTA
                     QSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMLDARA
                     LTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGY
                     LQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKG
                     SEEKQN"
     gene            complement(1543..2046)
                     /gene="tadA"
                     /locus_tag="ACHKIK_003110"
     CDS             complement(1543..2046)
                     /gene="tadA"
                     /locus_tag="ACHKIK_003110"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO9084233.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(2104..2739)
                     /gene="yfhb"
                     /locus_tag="ACHKIK_003111"
     CDS             complement(2104..2739)
                     /gene="yfhb"
                     /locus_tag="ACHKIK_003111"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO9084234.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2948..3796
                     /gene="yfhH"
                     /locus_tag="ACHKIK_003112"
     CDS             2948..3796
                     /gene="yfhH"
                     /locus_tag="ACHKIK_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO9084235.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3852..4112
                     /gene="yfhL"
                     /locus_tag="ACHKIK_003113"
     CDS             3852..4112
                     /gene="yfhL"
                     /locus_tag="ACHKIK_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO9084236.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(4306..4386)
                     /gene="shoB"
                     /locus_tag="ACHKIK_003114"
     CDS             complement(4306..4386)
                     /gene="shoB"
                     /locus_tag="ACHKIK_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO9084237.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(4806..5186)
                     /gene="acpS"
                     /locus_tag="ACHKIK_003115"
     CDS             complement(4806..5186)
                     /gene="acpS"
                     /locus_tag="ACHKIK_003115"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO9084238.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(5186..5917)
                     /gene="pdxJ"
                     /locus_tag="ACHKIK_003116"
     CDS             complement(5186..5917)
                     /gene="pdxJ"
                     /locus_tag="ACHKIK_003116"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO9084239.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(5929..6657)
                     /gene="recO"
                     /locus_tag="ACHKIK_003117"
     CDS             complement(5929..6657)
                     /gene="recO"
                     /locus_tag="ACHKIK_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO9084240.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(6669..7574)
                     /gene="era"
                     /locus_tag="ACHKIK_003118"
     CDS             complement(6669..7574)
                     /gene="era"
                     /locus_tag="ACHKIK_003118"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO9084241.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(7571..8251)
                     /gene="rnc"
                     /locus_tag="ACHKIK_003119"
     CDS             complement(7571..8251)
                     /gene="rnc"
                     /locus_tag="ACHKIK_003119"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO9084242.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(8524..9498)
                     /gene="lepB"
                     /locus_tag="ACHKIK_003120"
     CDS             complement(8524..9498)
                     /gene="lepB"
                     /locus_tag="ACHKIK_003120"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO9084243.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(9514..11313)
                     /gene="lepA"
                     /locus_tag="ACHKIK_003121"
     CDS             complement(9514..11313)
                     /gene="lepA"
                     /locus_tag="ACHKIK_003121"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO9084244.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(11511..11990)
                     /gene="rseC"
                     /locus_tag="ACHKIK_003122"
     CDS             complement(11511..11990)
                     /gene="rseC"
                     /locus_tag="ACHKIK_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO9084245.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKVSCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(11987..12943)
                     /gene="rseB"
                     /locus_tag="ACHKIK_003123"
     CDS             complement(11987..12943)
                     /gene="rseB"
                     /locus_tag="ACHKIK_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO9084246.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(12943..13593)
                     /gene="rseA"
                     /locus_tag="ACHKIK_003124"
     CDS             complement(12943..13593)
                     /gene="rseA"
                     /locus_tag="ACHKIK_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO9084247.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(13626..14201)
                     /gene="rpoE"
                     /locus_tag="ACHKIK_003125"
     CDS             complement(13626..14201)
                     /gene="rpoE"
                     /locus_tag="ACHKIK_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO9084248.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(14198..14353)
                     /gene="rseD"
                     /locus_tag="ACHKIK_003126"
     CDS             complement(14198..14353)
                     /gene="rseD"
                     /locus_tag="ACHKIK_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO9084249.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            14609..16231
                     /gene="nadB"
                     /locus_tag="ACHKIK_003127"
     CDS             14609..16231
                     /gene="nadB"
                     /locus_tag="ACHKIK_003127"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO9084250.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(16216..16953)
                     /gene="trmN"
                     /locus_tag="ACHKIK_003128"
     CDS             complement(16216..16953)
                     /gene="trmN"
                     /locus_tag="ACHKIK_003128"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO9084251.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            17085..18419
                     /gene="srmB"
                     /locus_tag="ACHKIK_003129"
     CDS             17085..18419
                     /gene="srmB"
                     /locus_tag="ACHKIK_003129"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO9084252.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
BASE COUNT         4526 a         4885 c         4596 g         4448 t
ORIGIN      
        1 aaagattaat tatctgttca tcggcattct ggcactgctg ctcgcggtcg ctctctggcc
       61 atccattccc tggtttggta aagccgacaa ccgtatcgcc gccattcaag cgcggggaga
      121 gttgcgtgtg agcaccattc atactcccct gacttataac gaaatcaacg ggaaaccttt
      181 tggcctggat tacgaactgg cgaaacagtt tgccgattac ctcggcgtaa aactgaaagt
      241 gaccgtgcgg cagaatatca gccagctgtt tgacgacctt gataatggta acgccgacct
      301 gctggcggca ggactggtct ataacagtga gcgggtaaaa aattatcagc ctggccctac
      361 ctattattcc gtgtcacaac aactggttta taaagtgggt cagtatcgcc cacgtacgct
      421 gggcaacctg acggcggagc aactcaccgt tgcaccgggt catgtggtgg ttaacgatct
      481 ccagaccctg aaagaaacaa aattcccgga attaagctgg aaggtagacg acaaaaaagg
      541 ctctgcggaa ttaatggaag atgtcatcga aggaaaactc gattacacca ttgctgattc
      601 tgtcgccatc agcctgtttc agcgcgttca cccggagctc gccgtagcgc tcgatatcac
      661 cgatgaacaa ccggtgactt ggtttagccc gttagatggc gataataccc tttccgccgc
      721 cctgctcgac ttcttcaacg aaatgaatga agacggtacg ctggcacgca ttgaagagaa
      781 atacctgggg catggcgatg attttgatta cgtcgatacg cgcacatttt tacgcgccgt
      841 cgatgcggta ctgccgcagt taaagcccct gtttgagaaa tacgccgaag aaattgactg
      901 gcgtttgctg gccgctattg cttatcagga atcgcactgg gatgcacagg ccacttcacc
      961 gacgggtgtg cgcggcatga tgatgttaac caaaaatacc gcgcaaagcc tcggcattac
     1021 ggatcgtacc gatgccgaac agagcatcag cggtggcgtg cgttatttgc aggatatgat
     1081 gagtaaagtg ccggaaagtg tgccggagaa cgagcggatc tggtttgccc tcgctgcgta
     1141 caatatgggc tatgcgcata tgctggatgc ccgcgccctg acggcaaaaa ccaaagggaa
     1201 tcctgacagt tgggctgacg taaaacagcg tctgccttta cttagccaga aaccctatta
     1261 cagcaagctg acttacggct acgctcgtgg acatgaagcc tacgcttatg tcgaaaatat
     1321 tcgtaagtat cagattagcc tggtgggtta tctgcaagag aaagagaagc aggctacaga
     1381 agcggcgatg caactggcgc aggattatcc ggcggtatcg cctacggagt tgggcaaaga
     1441 gaaatttcct tttctctcgt ttctttccca gtcgtcatca aactatttga cccattctcc
     1501 ctctctgctg ttttccagga aagggagtga agagaaacaa aattaatccg tcgaggattg
     1561 cgcttttttc tgcgctttaa tttcctggcg gcgcatgcga aagaagtcac tgagcaacgc
     1621 cgcgcactca tccgccagta ttccttccgt aatttccact cggtgattca tacccggatg
     1681 atgcagcaca tccattaaag atcccgcagc gccagttttc gcgtcacgcg caccaaagac
     1741 cacgcgacca atgcgactgt ggatcatcgc tccggcacac attacacatg gttcaagcgt
     1801 gacatacaac gtggcgtcga tcagacgata attttgcatc accagaccac cctgccgcag
     1861 ggccatgatt tctgcatgtg cggtgggatc atggcgacca atcgggcggt tccagccttc
     1921 gccgattacc cgattgttat gcactaatac cgcgccgacc ggcacttccc gctcatccca
     1981 ggcacgtttc gccagcgtca gcgcgtgacg catccagtat tcgtggctaa attcgacttc
     2041 agacaaaaag aaaactccgg ttataaaagc gcggcgcatt atacacggac gctatgcttt
     2101 actctattcc agttgctgga gttcaccgcg cggggttact cgccaacgat gctgacagaa
     2161 ataaagcagc ggattgtcct gattactgtc gctatagcca ctgtacagcc gcagcggagt
     2221 gccgattttg cgctccagtt gtgcgacctt ttcatgtccc agacaacgca tcgtcaatac
     2281 ccaaccacca tagccacgct gaatttggct ggcgataaga ttaacccgcg gcagccaggg
     2341 cgtatcgaaa taaaccgctt caaccagcgg ctgcggagag ccggtaatca accagatatc
     2401 agcatcggaa cttaacaggt aggtggttaa tcgctcctga accagcggaa aggcggtaac
     2461 attgtcgcga aaccagcgca cgaaatcggc ctgcaacgtc tgtaaacgtg cttcgctgtg
     2521 accaaaagtg cacccccaca gaagcagact catcggccag cgtgccgcac gaccttttat
     2581 caataacgca atggctataa tcggtaacaa cggcaggaca agtaacgcat tcagcggttg
     2641 gcgacgcagt aaatagcgca gaaaactgcc gaacatatcc tgctgatgca atgttccatc
     2701 taagtcaaaa aacaccacac gacgctcgtg agttgccaaa ccatactcct ctggactgaa
     2761 acatcctgat taattttcgc tactacatag cctaacagat agatcatcac ttttccggca
     2821 acaatcgacc gcatcatggc taactggaat ttttaattca tggcaattag cggcaatgga
     2881 atataaaatt cactcgcgtg tctctcatat tgcctgttgt cgccactatt tcatcgcgag
     2941 gaaagatatg aacggcttac ttcgtatccg tcagcgttac caggggcttg cccagagtga
     3001 taaaaaactg gcggattatc tgctgctaca acctgatacg gcgcgccatt taagctctca
     3061 gcaactggcc aacgaagccg gagtcagtca gtccagcgtc gtgaagttcg cgcaaaaact
     3121 cggctataaa ggttttccgg cgcttaagtt ggcgttgagt gaagcgctgg caagccagcc
     3181 ggaatcgccc tccgtgccca ttcataacca aatccgcggt gatgatccgt tacggctggt
     3241 cggcgaaaaa ctgattaaag aaaatactgc cgctatgtac gcaacgctaa acgttaatag
     3301 tgaagagaaa ctgcatgaat gcgtaacaat gttgcgctct gcgcggcgga taattctgac
     3361 cggtattggc gcttcgggtc tggtggcgca aaactttgcc tggaagctga tgaagattgg
     3421 cttcaatgct gccgcagtgc gcgatatgca tgcgctactc gcaacagtac aggcgtcgtc
     3481 ccctgacgat ctgttattag ccatttccta caccggtgta cgacgcgagt taaacctggc
     3541 ggcagatgag atgctgcgag tgggcggaaa agtgctggcg attaccggct ttactccgaa
     3601 tgccctgcaa cagcgtgctt ctcattgcct gtataccatt gccgaagaac aagcgacaaa
     3661 cagcgcttca atctctgctt gtcacgctca gggaatgtta acggatttgc tgttcattgc
     3721 gctgattcag caggatctgg aactggcacc agaacgtatt cgtcatagtg aagcgctggt
     3781 gaaaaaactg gtctgagtaa agaatgcgcg tataatgccc gcccggtttg tgttgttttg
     3841 agagtttcct tatggcgttg ttaatcacta aaaaatgcat caattgtgat atgtgtgaac
     3901 ccgaatgccc gaatgaggcg atttcaatgg gagatcatat ctacgagatt aacagcgata
     3961 agtgtaccga atgcgtaggg cactacgaga caccaacctg ccagaaggtg tgcccgatcc
     4021 ccaatactat tgtgaaagat ccggcgcatg tcgagacaga agaacagttg tgggataaat
     4081 ttgtgctgat gcaccacgcg gataaaattt aatattctac tctggaagta gagtattaat
     4141 tatattactg ggaagcctta acgccattat atttatttaa ttgatgacat tagcataatc
     4201 attcactaag ttaatttata tagtatctgc caagacactt atttatagtt attaaaggcg
     4261 cgtccgattg gttcaccgga cgcaccttaa gtacgtttcc ttgtgttata agaacagaag
     4321 gatcagctgt aaaacagcaa tgatgatttt gatgacccgt ttaatcaggt atcggcaatc
     4381 agtcattcgt ttttccttaa acaaggaatg cagccgttca gattagccct tacatctccc
     4441 caaaactgaa cgtgcgagtt attgagggtg catgctgcac tccacaccag agctttgacg
     4501 acaccactcg tttcaatggg ggaattctgt ggcatggtgt aaagcacagc aaaatcttca
     4561 ataacgaagc caattttaat gtacttacga attggcggtc acgttaatct ttccatcaat
     4621 attgctttct tcgtaaaggc tcgagttttt atgctaaaga ttgcaagttg cttgtaaaag
     4681 ataagtacac tgatccataa tcgctgttgt tgagggtgca tgctgcacaa aattaaagtt
     4741 aaaaagtaaa acccccgttc cttaccagtt cgggggtttt actttttaaa gagaacggta
     4801 ttattttaac tttcaataat taccgtggca caagcatagt ggcgttcatc tgccagcgtt
     4861 acatgcatat ttacaacgcc gagcttttcc gccagtttta atgcctcacc ccatagccgt
     4921 agccgtggtt tgccgagctc atcattgaat acttcaaatt gattaaacgc cagaccattg
     4981 cggatcccgg tgccaaacgc ttttgctgcg gcttctttca cagcaaaacg cttcgccaga
     5041 aaacgcaccg gctggtggtg cgttttccag attgcccatt cgttatcgct taatacgcgg
     5101 cgtgccaggc gctcaccgga tcgggcgatc actgcttcta tgcgagcgat ctctacgata
     5161 tccgtgccta aacctaatat tgccattagc cacgcgcttc cagcatcaga cgcttcattt
     5221 ctgccaccgc atctttcagt ccggtcatca ctgcacgacc aataatggca tgaccgatat
     5281 tcagttcatg catctcaggg atggcggcaa tagctttcac gttgtgatag gtcagaccgt
     5341 gaccagcgtt aactttcaga ccgagactag cggcaaaggt cgcggctttg gcgatacgcg
     5401 ccagctcttg cgcctgttcg gcgtcagttt tggcatcagc atagcaaccg gtgtggatct
     5461 cgataaacgg tgcgccaacc tctgccgcag ctttgatctg ctcttcatcg gcgtcgataa
     5521 agagagaaac ctggatcccg gcatctgcca gacgtttgca ggcatcgcgc attttgtcac
     5581 gctgccctgc gacgtccagg ccgccttcgg ttgttacttc ctgacgcttt tccggtacca
     5641 gacagcaaaa atgtggcttc gtctcaacgg cgatcgccag catctcttcg gtcaccgcca
     5701 tctccagatt catgcgggta tccagcgtct gacgcaggat gcgcacatca cggtcagtaa
     5761 tgtggcggcg atcttcacgt aaatgcacgg taatgccgtc cgctcccgcc tgctcagcaa
     5821 taaacgccgc ctgcaccgga tccgggtaag cagtaccgcg tgcgttacgc agcgttgcaa
     5881 tatggtcaat gttgacgcct aacagtaatt cagccatgac aatcctcatc attcataatg
     5941 tgttttcacc gttcgcttag gcataaactg ccggaacagt tccctgctct ttaatggttt
     6001 accgccaaga tacggcttaa gcgccatgcg ggtaaagcgt ttcgcggcgc gtagtgtgtc
     6061 tgcgtcagga aattcccgcg cgtttaacgc ttttaactgc cttccggtga acgttttatt
     6121 gtcgataacg acgcttgcga taaacccttt ttcttcgcga taacgatacg tcatggtgtc
     6181 atctaccggc tcgccgctac ccgcacaatg ggtaaaattg acgccatagc ccagatgccc
     6241 gagcagtgcc agttcaaagc ggcgcagcgc gggttctggc gtaccagtgg cacctgcaag
     6301 agactgaatg cagtgcaagt aatcgaaaaa gagttcagag aagcgcgtct cgtattccag
     6361 tacgcgggag agaagttcgt tgatgtacag accgctgtaa agcgtgatac cgcttaatgg
     6421 cagcgccagc gagacagctt cagcactgcg cagcgttttg acttcgccac gcccgccgaa
     6481 acgtagcaag agaggggtga aaggctgtaa tgcacctttc agagtagagc gtttagagcg
     6541 tgcgcctttg gcaaccagac gcacgcgacc cgattcctcc gtgaagacgt ccagcatcag
     6601 gctggtttcg ctccacgggc gactatgcag gacaaatgcg cgctgccagc cttccatcgg
     6661 agtgactctt aaagatcgtc aacgtaaccg agactgcgca gtgcgcgttc gtcgtcggcc
     6721 caaccggatt tcacttttac ccacagttca aggtggacag gcgcttcgaa catttcctgc
     6781 atgtctttac gcgcttcaat cccgatggtt ttgatcttgg cccctttgtt gccaataacc
     6841 atcttcttct gcccttcacg ctcaacgaga atcaaaccat tgatgtcata accaccgcgt
     6901 tcgttagaga cgaaacgttc gatctccacg gtcacggagt acggcagttc agcgccgagg
     6961 aaacgcatca gtttttcgcg gatgatttca gacgccataa aacgctgtga gcgatcggtg
     7021 atgtaatctt ccgggaagtg atgggtcgct tcagggagat gcttacgcac gattgctgcg
     7081 atagtgtcga cattcagccc ggtttcagca gagatcggca cgatatcgag gaagttcatc
     7141 tgacttgcca ggaactgtaa gtgcggcagc agatcggctt tttcctgcac gttatccact
     7201 ttgttcaccg cgaggatcac cggcgctttg ccatcgcgca gtttgttgag caccatttcg
     7261 tcgtccggcg tccagcgggt gccttcaacg acaaaaatca ccagctcaac gtcgccaata
     7321 gagctgctcg ccgctttgtt catcaggcgg ttaatagcgc gtttttcttc catatgcagg
     7381 ccaggggtat caacgtagat cgcctgatac gcgccttcag tatggatccc cacaatgcgg
     7441 tgacgagttg tttgcgcttt gcgggaggtg atggagatct tctgccccag cagtttgttc
     7501 aacaatgtgg atttgccaac gttcggacgt ccgacgatgg caataaatcc gcagtaactt
     7561 ttatcgatgc tcattccagc tccagttttt tcaacgcctg ttcggcggca gcctgctcag
     7621 ccttacgacg gcttgaacct gtgccaacca ccggttcact caggccgctg acctggcagt
     7681 ggatagtaaa ttcctgatcg tgcgcttcgc cacgtacctg gactaccaga taagtcggca
     7741 gcggcagatg gcgaccctgc aaatattctt gcaagcgcgt tttcggatct ttttgtttat
     7801 cgcctgggct aatttcgtcc aaacgagttt gataccagtt gaggattaat ttctcgacgg
     7861 tttgaatatc actgtcgagg aatacaccac caattaatgc ttcgacggtg tcggcgagaa
     7921 ttgactcacg acgaaatcca ccgcttttaa gttcacctgg ccctaaacgt aagcactcgc
     7981 ctaactcaaa ttcgcgcgcc agttccgcca gcgtattgcc acggaccagc gtggcgcgca
     8041 tccggctcat atcgccttca tccacacgag ggaaacggtg ataaagcgca ttggcgataa
     8101 cgtagctcag aatagagtcg cctaaaaatt ctaaacgctc gttatgtttg ctgctggcac
     8161 tacgatgagt taatgcctgc tgcaacagtt cctgatgatt aaaagtgtag cccagcttcc
     8221 gttgaagccg attaattacg atggggttca tgcgatacca ataaataaat gcgtcaacaa
     8281 ttcagcacac gaaacagacc tgatatacat ggctctgcta actgcttcgc tgcagtttca
     8341 gtttaccggt atatggggac caacgctgtt tcgtgtgccg tggcaacctg gaggtgccaa
     8401 ccttaaactt cgggggaata ttctatacac aacgacgggg gatgtcgtta gccacgggag
     8461 atttatctca taaataattc acgttgtcgc cataacggcg acaacgtgaa cgaagatggg
     8521 ctattaatgg atgccgccaa tgcgacttaa gcgcacacct gtcggccatt cgccttcttg
     8581 cttatcgaaa ctcatccaga tagccgttgc ccgaccgacc agattcgctt caggcacaaa
     8641 accccagtaa cggctgtccg cgctgttgtc gcggttgtcg cccatcatga agtattgtcc
     8701 cggcggaaca atccaggttg ccagttgttg ccctggctgc tggtaataca tccccacctg
     8761 gtcctgcgca atcggcactg tcagaattcg gtgcgtcaca tcacccagtg tctctttacg
     8821 ctcggaaaga cgaattccat tttctttggt ttcgtttttc ggcacttcaa agaatccgct
     8881 ggtcgcttcc ccaccattac ggcgtgagaa ggtctgaacg aaatcgctcg gttccacgtt
     8941 ggagtaggtg accggtagcg cgttttcaca cgcctggccg gaactgcatc ccggttgaat
     9001 cgtcagctct tttgagaccg gatcgtaagt gactttatcg cccggtaaac ccaccgcgcg
     9061 cttgatgtaa tcaagctttg gatcttccgg atatttaaag accacgatat cgccgcgttt
     9121 cggatgaccg gtttcgatca gcgttttctg gtagatagga tctttaatgc cataagcaaa
     9181 cttctctacc agaataaaat caccgattaa cagagtcggc atcatcgaac ctgacgggat
     9241 ctggaacggt tcataaataa acgaacgtac aatcaatacg atagccagca ccggaaaaac
     9301 agaagcaccg gtttccagcc agccaggctt cggcgcaacc tttttcaggg ttgctttatc
     9361 cagtgagtca ccggcagccg cctgcgccgc tgcctgacgt tcccgccgtt taggtgcgaa
     9421 aaagaattta tccacgcacc ataaaatgcc cgtcaccagt gtggcaatca ccagaatcag
     9481 ggcaaacata ttcgccatgc caactcctaa gggttatttg ttgtctttgc caacgtgcag
     9541 aatggcgagg aacgcttcct gcggcagctc gacgttaccg atctgcttca tgcgtttctt
     9601 accttctttc tgcttctgca gcagcttttt cttacggctg atatcgccgc cataacattt
     9661 agccagtacg tttttacgta gctgtttcac ggtggatcgc gcaataatgt gcgtaccaat
     9721 cgctgcctga atggcgatat caaactgctg gcgtgggatc agatctttca tcttctccac
     9781 caactcgcga ccgcggtttt gcgaattatc acggtgggtg atcagcgcca acgcatcaac
     9841 acgttcaccg ttgattaata cgtctacacg taccatgtcg gacgcctgga agcgtttgaa
     9901 gttgtaatcc agagacgcat aaccacgcga ggtagatttc aggcgatcga agaagtcgag
     9961 caccacttcc gccatcggga tctcgtacgt cagcgccacc tgattaccgt ggtaaaccat
    10021 attggtctgc acgccgcgtt tttctacgca cagcgtaata acgttgccga gatatgcctg
    10081 cggcagcagc atgtgacatt cggcaatcgg ctcgcgcagt tcgtagatgt tatttaccgc
    10141 tggcagcttg gatggactgt cgacgtaaat aacttctctt gacgtggttt caacttcata
    10201 cactacggtc ggcgcagtgg tgatcagatc cagatcgtat tcacgttcca gacgttcctg
    10261 gatgatctcc atgtgcagca ggccgaggaa gccgcagcgg aaaccaaagc ccagcgcgct
    10321 ggagctttcc ggctcataga acagtgaagc atcgttcagg ctgagtttac ccagcgcgtc
    10381 acggaaggct tcatagtcgt cggaacttac cgggaacaga ccggcgtata cctgcggttt
    10441 gactttctta aagccaggca gcgccttttc tgccggatta cgcgccagcg ttaaggtatc
    10501 gccgactgga gcgccgtgga tatctttaat cgcacatacc agccagccta cttcgccaca
    10561 tttcagttca gtgcggtcaa cctgtttcgg cgtgaagatg cccagacggt cggcgttata
    10621 ggtctgcccg gtactcatga ctttcacttt gtcgccctta cgcagggtgc cgtttttaat
    10681 acggataagt gaaacaacgc ccaggtagtt gtcgaaccat gagtcgataa ttagtgcctg
    10741 caacgggcct tccggatcgc cttccggcgg cggaatgtcg cgcaccagac gttcgagaac
    10801 gtcctgcacg ccaacgccgg ttttcgctga acagcgcacc gcgtcggtgg cgtcgatgcc
    10861 gacgatatct tcaatttctt ccgccacgcg ttcaggatcg gctgccggca ggtcaatctt
    10921 gttcagtacc ggcaccactt cgagatccat ttccatggcg gtgtagcagt ttgccagggt
    10981 ttgcgcttct acgccctgcc cggcgtcgac caccagcaat gcaccttcac aggcagccag
    11041 cgaacgggaa acttcatagg agaagtcaac gtggcccggg gtgtcgataa agttcagctg
    11101 ataggtttcg ccgtcagacg ctttgtagtc cagcgtcacg ctttgcgctt tgatggttat
    11161 gccacgctca cgctcaagat ccatggaatc gagaacctgc gcctccattt cacggtcaga
    11221 caggccaccg cagatctgga taatacggtc agacagcgtc gatttaccgt ggtcaatgtg
    11281 agctatgatc gaaaagttac gtatattctt cataaagtat gattattgtg ccttaatgcc
    11341 cggttaacca ggcttttaga agtcgctgtt ctgagcttaa cgtctgtatt aatagaaacg
    11401 ccgcattcta cactacaaca ttgaggcgag gaaatgttca taccgtatgg attgtggtat
    11461 ctggaaacgt cctcgcattt gttatgcaaa atgcaacaaa gccagtgaaa tcactggctc
    11521 gcgtcttccg aagatgtttc aaatcgcaca aggccaggcg gcaatgccac gcttaagatg
    11581 atcggttgcc attctgcccg ggcagcaaac ttacgcgagt agccgcgcgc aatcaggaac
    11641 ccgccaatac cgccgaggac cgcaccacat aatgctgcga cgtcagaagc aaagagtagc
    11701 tgaaatagcg aagcgatgag gaataatccc accagcggcg acatataaac cagtaatgcg
    11761 gagctaagca ggctgccttc ggcgatcccc aattccactt tttgccccgg cactaacggc
    11821 tcatcacagg gtacgacaat ggtatgcgtg gtttgcgggc caagtttatt taacacgcgg
    11881 ctaccgcaac cggcgcgtga agcgcagctg ctgcatgaaa ctttaacatc acaactgacc
    11941 agcgcctgcc cgttttgcca ggagacgacg gtagcccact ctttgatcat tgcgctgccc
    12001 cgaacttaat attctcggca atgcgtttcg ccgtttgcgg cggcagttca ccgacaatgg
    12061 tgatttcggc gttatcacgt acgcttgtac tgacggttct gcgtccggtg cgcaacatct
    12121 gatcggtgct cgatggcgta gcgcggttaa cgtttaccga gaagctgaat aatccgtcgg
    12181 aatagagacg tgattcgata ggcatgttgt ccatcgtcgg tagcggacgt cgactactgg
    12241 aaacttcgct aaaaccctgt ggcaaccagg ttggcgtcca gctgaattta gctttttcac
    12301 ctacaggaac agaaagcaac ggcggcaaat ttgccttcgc cagcgtctgc atactgctac
    12361 tgatatcctg attgacgtta aaagcaatca cgcgaaattg ttccagcgtt tcaccatcgc
    12421 gatcaaggag atcaaccctc atcggtaatt tcgattcgat gtccatccac acgatgtagc
    12481 tgtagcgtgt accatctcgg gcaaccacgc gaatgacttc gcaaagacga tcagcaatac
    12541 gcgtgcgccc gacggagata aagtcgtagt aaggagaaag gcgtttgaaa tcggtataga
    12601 tgagcgatgg cagagaatca acaatgtaat cgccattaag cgtgaacggt tcaagtcccg
    12661 gttcaaaata gctgatttca ttgccacgct gtaccacttc ccggcgcggg ccatccattt
    12721 gcaacaattg tgcaagagga cggttatcga ggcgtgcatg tcgataacgc agagactcaa
    12781 caccctgttt attgatgctg atgaatgaca gctcgtaatt cagtgactga ctggccaggt
    12841 tcatctgctg taataacgcc ccggacgcgg gagtggccga ggcgttagca gagaataaca
    12901 ggctacctgt cactaatgac atggcaaacc aaagttgctt cattactgcg attgcgttcc
    12961 taaagtttga attcctggca cctgtacagc ggcttgctgg gtttgcgcct gctcaaactg
    13021 aagctgttca gagtggagtc ggcgttgcag ttcgtaatcc tgcaacattg cattaatgcg
    13081 acgacgctgc tcctgtacct gctgctgttg accattgttt gcggtcgctt cagaaggtac
    13141 tcccaggctt accgggctgg ctttacccat catcggcagt gtattaaata ccggcgtttc
    13201 gggctgctgg gacgtttcag attgtccatt atagtgctgg acgccaacga taactgcaag
    13261 cgatacgcat gctgctacgc ccatttgggt aagctgtgcc gcccacggac gtactttctg
    13321 ccagaatggc attttctgcc attgatgcgg cgcaggctgg gcttccggga tcaatgtcgc
    13381 cggttgacgt actggctctt cttcaatggc ggccatcacg cgtgaagaga tatcgaaatg
    13441 gagcacctcg ggagtatcac cccgcattga gtcacggatt aagtgatagc tttcccaggt
    13501 tttctgcatt tctgggttat gagccagttc gttaagcagc tcactatcca gcgtttcgcc
    13561 atccattaaa gcggaaagtt gttctttctg catgcctaat acccttatcc agtatcccgc
    13621 tatcgtcaac gcctgataag cggttgaact ttgttatcaa tagcttccct cgctcggaag
    13681 atacgtgaac gcaccgtacc taccggacaa tccatgatag cggctatctc ttcatagctc
    13741 aggccatcca gctcccgcaa ggttattgcc atgcgcaaat cttccgggag ggactcaata
    13801 gttcggaaaa ctatctgtct cagttcttct gacaacatta agttctcagg gttcgaaatt
    13861 tctttcaacg cgccgccact ttcgaagttt tcagcttcaa tggcatccac atcactggaa
    13921 ggtggacgac gcccctgagc aaccaggtaa tttttcgctg tatttacagc aatccgatac
    13981 agccatgtat aaaaagcgct atctccccgg aacgaatcca gcgcacgata ggctttaata
    14041 aaagcttctt gtaccacatc gggaacatca cccgacggca catagcggga aaccagactc
    14101 gccactttat gctgatagcg cactaccagt aggttaaagg ctttctgatc tcccttctgg
    14161 acccgttcaa ccaggacctg gtccgttaac tgctcgctca tccgaggtaa agtctcccca
    14221 aaccaaattt ccacgcgcta tcgaaacgcc actccattag ctgcaatttg agcaagcaaa
    14281 gggttagagt gtctcgtttt tgtaaagttc cgtaacgcat ctgtttttgt ttgtcatgct
    14341 gtagacggat cattatctat cattataagt ctacagaatc tgaacatcgc attatctgtg
    14401 tagaaatgcc catttaactg cctgaagagt aacccaacgg cctttttatt tcaccaccta
    14461 atcctccacc agccagtaac ttctcttttt ctcgccgccc tgcgtcagcg tgtttagcca
    14521 ctgtaacaaa tattaaaata gcaggtgttt attcgcacaa catgatgcta tgctgaccaa
    14581 acggtgttta gtaaattaaa caaagaaaat gaatactctc ccagaacatt catgtgacgt
    14641 gttgattatc ggtagcggcg cagccggact ttcactggcg ctacgcctgg ctgaccagca
    14701 tcaggtcatc gttctaagta aaggcccggt aacggaaggt tcaacatttt atgcccaggg
    14761 cggtattgcc gccgtgtttg atgaaactga cagcattgac tcgcatgtgg aagacacatt
    14821 gattgccggg gctggtattt gcgatcgcca tgcagttgaa tttgtcgcca gcaatgcacg
    14881 atcctgtgtg caatggttaa ttgaccaagg ggttttgttt gatacccacg ttcaaccgaa
    14941 tggcgaagaa agctaccatc tgacccgtga aggtggacat agtcaccgtc gtattcttca
    15001 tgccgccgac gccaccggta gagaagtaca atccacgctg gtgagcaagg cgcagaacca
    15061 tccgaatatt cgtgtgctgg agcgcagcaa cgcagttgat ctgattgttt ctgacaaaat
    15121 tggcctcccg ggcacgcgac gggttgttgg cgcgtgggta tggaaccgaa ataaagaaac
    15181 ggtggaaacc tgccacgcaa aagcagtggt actggcaacc ggcggtgcgt cgaaggttta
    15241 tcagtacacc accaatccgg atatttcttc tggcgatggc attgctatgg cgtggcgcgc
    15301 aggctgtcgg gttgccaatc tcgaatttaa tcagttccac cctaccgcac tgtatcaccc
    15361 acaggcacgc aatttcctgt taacggaagc actgcgcggc gaaggcgctt atctcaagcg
    15421 cccggatggc acgcgtttta tgcccgattt tgatgagcgc ggcgaactgg ccccgcgcga
    15481 tattgtcgcc cgcgccattg accatgaaat gaaacgcctc ggcgcagatt gtatgttcct
    15541 cgacatcagc cataagcccg ccgattttat tcgccagcat ttcccgatga tttatgaaaa
    15601 attgctcggg ctggggattg atctcacaca agaaccggta ccgattgtgc ctgctgcaca
    15661 ttatacctgc ggtggtgtaa tggttgatga tcatgggcgt acggacgtcg agggcttgta
    15721 tgccattggc gaggtgagtt ataccggctt acacggcgct aaccgcatgg cctcgaattc
    15781 attgctggag tgtctggtct atggctggtc ggcggcagaa gatatcacca gacgtatgcc
    15841 tgatgcccac ggcgtcagta cgttaccgcc gtgggatgaa agccgcgttg agaaccctga
    15901 cgaacgggta gtaattcagc ataactggca cgagctacgg ctgtttatgt gggattacgt
    15961 tggcattgtg cgcacaacga agcgcctgga acgcgccctg cggcggataa ccatgctcca
    16021 acaagaaata gacgaatatt acgcccattt ccgcgtctca aataatttgc tggagctgcg
    16081 taatctggta caggttgccg agttgattgt tcgctgcgcg atgatgcgca aagaaagccg
    16141 ggggctgcat tttacgctgg attacccgga actgctcacg cattccggtc catcgatcct
    16201 ttcccccggc aatcattaca taaacagata aaaagcctgg gtcagcgccg tatacgcttc
    16261 ggaatagttc tggtctggcc cacgaatgac taagcgatcg ctaaagcatt ctcccgcctg
    16321 cggggagaat gccagcagca cccgatgcgg cagtcgcgct tcgttttccg ccacatccgt
    16381 ccgcaaacgt aaatgccagc ccatgcttaa tgccagttca gtaaaaccat taccaatctg
    16441 ctctggcagc accacgcaga aaaatccctc ttcggtaatg cactccgccg cacaggtcag
    16501 cagcgatggg tgatcaagcg tagtggtata gcgagcctgt tcccgttgag gtgtcgcgca
    16561 ctctactccc tgctgatagt aaggtgggtt actgatgatt aaatcgaagc gtactgtctg
    16621 ctgtgtgatc cactgctgaa tatccgccgt atggacgtta atccgctctg cccacgggga
    16681 ctggttgata ttttcctgcg cctgcgcggc agcttcactt tccagctcaa cggcatcaat
    16741 tattacgctg tcactggttc gctgcgccag catcagtgcc agcaacccgc tacccgcgcc
    16801 gatatcaagg caacgtttca ccccagccac cggtgcccat gcgcccagca aaatgccatc
    16861 cgttcccact ttcatcgcac agcgatcgtg agcaacaaaa aactgtttaa aagtaaatcc
    16921 attacgacga agcacggatg tagactgtga catgaaaata aaaccttgca ggaaaaacgg
    16981 cgatagcacc gggtgagaac aatacccgag aagcgatatc catacaaaca gatgaagatt
    17041 gcagccgtaa cgtctataat cagcgcccca cacagaggta gaacatgact gtaacgactt
    17101 tttccgaact tgaactcgac gaaagcctgc tggaagccct ccaggataaa ggtttcactc
    17161 gcccgaccgc cattcaggct gccgccattc cgcctgcgct cgatggccgt gatgtactcg
    17221 gttctgcgcc gacaggcacc ggtaaaacgg cggcgtatct gctgccagcg ttgcagcacc
    17281 tgctcgattt cccgcgtaag aaatccggtc cgccgcgtat tttgatcctc accccaactc
    17341 gcgagctggc gatgcaggtg gccgatcatg cccgcgaact ggcgaaacat acgcatctgg
    17401 atatcgccac catcaccggc ggcgtagcct atatgaacca cgcggaagtg ttcagcgaaa
    17461 atcaggacat cgtggtcgcc acgaccggac gtctgctgca atacataaaa gaagagaact
    17521 tcgattgccg cgcggttgaa acgctgatcc tcgacgaagc agaccgtatg ctggatatgg
    17581 gcttcgctca ggatatcgaa catattgctg gcgaaacgcg ctggcgtaaa cagaccctgc
    17641 tcttttcggc aacgctggaa ggcgatgcga ttcaggactt tgccgagcgt ctgctggaag
    17701 atccggtgga ggtttctgcc aatccgtcca cccgtgagcg caaaaaaatt catcagtggt
    17761 attaccgcgc cgatgacctg gaacataaaa ccgcattgct ggtgcatctg ttaaaacagc
    17821 cggaagcgac ccgctcaatt gtgtttgtgc gtaagcgtga acgtgtgcat gagctggcaa
    17881 actggctgcg cgaagcgggc atcaacaact gctatctcga aggtgagatg gtacaaggca
    17941 agcgtaacga agcgatcaag cgtttgaccg aaggtcgcgt aaacgtactg gttgcaaccg
    18001 atgttgccgc gcgcggtatc gacattcctg acgtcagcca cgtctttaac ttcgatatgc
    18061 cgcgcagtgg cgatacttat ttgcaccgta tcggacgtac cgcgcgcgcc ggtcgtaaag
    18121 gcaccgcaat ttcgctggtg gaagcccatg accatttgct gctgggcaag gttggccgtt
    18181 acattgaaga gccgattaaa gctcgcgtta ttgatgagtt acgcccgaaa acgcgtgcgc
    18241 caagcgagaa gcagaccggc aagccatcga agaaagtact ggcaaaacgc gccgagaaga
    18301 aaaaagctaa agagaaagag aaaccgcgtg tgaaaaaacg ccatcgcgac accaaaaata
    18361 ttggtaagcg ccgtaaacca agcggaacgg gcgtgccacc gcaaacgaca gaagagtaat
    18421 ctcaatgccg ggtttaagcc tggtattaaa agtgc
//
LOCUS       DAZGJW010000089        17880 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZGJW010000089 DAZGJW010000000
VERSION     DAZGJW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.089"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..230
                     /gene="dinI"
                     /locus_tag="ACHKIK_003130"
     CDS             3..230
                     /gene="dinI"
                     /locus_tag="ACHKIK_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO9084253.1"
                     /translation="MAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRYAAANNL
                     SVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            520..774
                     /gene="bssS"
                     /locus_tag="ACHKIK_003131"
     CDS             520..774
                     /gene="bssS"
                     /locus_tag="ACHKIK_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO9084254.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSELEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            889..2007
                     /gene="solA"
                     /locus_tag="ACHKIK_003132"
     CDS             889..2007
                     /gene="solA"
                     /locus_tag="ACHKIK_003132"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO9084255.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTVIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            2055..2168
                     /locus_tag="ACHKIK_003133"
     CDS             2055..2168
                     /locus_tag="ACHKIK_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO9084256.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2429..2995
                     /gene="yceJ"
                     /locus_tag="ACHKIK_003134"
     CDS             2429..2995
                     /gene="yceJ"
                     /locus_tag="ACHKIK_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO9084257.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2999..3574
                     /gene="yceI"
                     /locus_tag="ACHKIK_003135"
     CDS             2999..3574
                     /gene="yceI"
                     /locus_tag="ACHKIK_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO9084258.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3616..4668)
                     /gene="trhO"
                     /locus_tag="ACHKIK_003136"
     CDS             complement(3616..4668)
                     /gene="trhO"
                     /locus_tag="ACHKIK_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO9084259.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4893..5813
                     /gene="lpxL"
                     /locus_tag="ACHKIK_003137"
     CDS             4893..5813
                     /gene="lpxL"
                     /locus_tag="ACHKIK_003137"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO9084260.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPQQYMWLHRRFKTRPEGVPSRY"
     gene            5985..7211
                     /gene="mdtG"
                     /locus_tag="ACHKIK_003138"
     CDS             5985..7211
                     /gene="mdtG"
                     /locus_tag="ACHKIK_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO9084261.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            7294..7668
                     /gene="msyB"
                     /locus_tag="ACHKIK_003139"
     CDS             7294..7668
                     /gene="msyB"
                     /locus_tag="ACHKIK_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO9084262.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(7669..7896)
                     /gene="yceK"
                     /locus_tag="ACHKIK_003140"
     CDS             complement(7669..7896)
                     /gene="yceK"
                     /locus_tag="ACHKIK_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO9084263.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(8069..10612)
                     /gene="mdoH"
                     /locus_tag="ACHKIK_003141"
     CDS             complement(8069..10612)
                     /gene="mdoH"
                     /locus_tag="ACHKIK_003141"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO9084264.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10605..12140)
                     /gene="mdoG"
                     /locus_tag="ACHKIK_003142"
     CDS             complement(10605..12140)
                     /gene="mdoG"
                     /locus_tag="ACHKIK_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO9084265.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12534..13691
                     /gene="mdoC"
                     /locus_tag="ACHKIK_003143"
     CDS             12534..13691
                     /gene="mdoC"
                     /locus_tag="ACHKIK_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO9084266.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(13699..15180)
                     /gene="clsC"
                     /locus_tag="ACHKIK_003144"
     CDS             complement(13699..15180)
                     /gene="clsC"
                     /locus_tag="ACHKIK_003144"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO9084267.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(15122..15655)
                     /gene="ymdB"
                     /locus_tag="ACHKIK_003145"
     CDS             complement(15122..15655)
                     /gene="ymdB"
                     /locus_tag="ACHKIK_003145"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO9084268.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(15750..16061)
                     /gene="ymdA"
                     /locus_tag="ACHKIK_003146"
     CDS             complement(15750..16061)
                     /gene="ymdA"
                     /locus_tag="ACHKIK_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO9084269.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(16182..16514)
                     /gene="csgC"
                     /locus_tag="ACHKIK_003147"
     CDS             complement(16182..16514)
                     /gene="csgC"
                     /locus_tag="ACHKIK_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO9084270.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(16573..17028)
                     /gene="csgA"
                     /locus_tag="ACHKIK_003148"
     CDS             complement(16573..17028)
                     /gene="csgA"
                     /locus_tag="ACHKIK_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO9084271.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(17069..17524)
                     /gene="csgB"
                     /locus_tag="ACHKIK_003149"
     CDS             complement(17069..17524)
                     /gene="csgB"
                     /locus_tag="ACHKIK_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO9084272.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            complement(17653..>17880)
                     /locus_tag="ACHKIK_003150"
     CDS             complement(17653..>17880)
                     /locus_tag="ACHKIK_003150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084273.1"
                     /translation="PWTIFYCKKTRFVTACAQDISQQSATGKKNDCLVIFDSGKRRFK
                     RKQNIFCVDNSVFTWVLILWYEQKKKNTTRG"
BASE COUNT         4481 a         4537 c         4220 g         4642 t
ORIGIN      
        1 ccatagcgaa aacttctcca ttgccagctg gggctattga cgccctggct ggcgaacttt
       61 cccgccgtat tcagtatgcg tttcctgata atgaaggcca cgtatcggta cgttatgccg
      121 cagcgaataa tttatcggtt attggcgcaa caaaagaaga taaacagcgc attagcgaaa
      181 ttctccagga aacgtgggaa agcgccgatg actggtttgt cagcgaataa tatgcagtga
      241 tttttgtttt tgccgggtcg ccccggcttt tttattttac ttacaaattt tgagtacaaa
      301 atttctacac ttcctcaaaa taattgcaca atatgctaaa aaaatatgaa tgacatgctg
      361 ttttattgtt catataaatc ttaaaagcag aaatttatct gttcccccct ttaaaaattc
      421 ggtgaatacc cttacttatt ggtatactga agttgcttca gataaaaacg cattgaacct
      481 cgaataacgt tgtctagtaa cacgaattag ggggccatga tggaaaaaaa taatgaagtc
      541 attcagactc atccgctcgt agggtgggac atcagcaccg ttgatagcta tgatgcgctg
      601 atgttgcgtt tgcactacca gaccccaaat aagtccgagc tggaagggac tgaagttggt
      661 cagacgctct ggttaaccac tgatgttgcc agacaattta tttcgatatt agaagcagga
      721 atcgccaaaa ttgaatccgg tgatttcccg gtaaacgagt atcggcgtca ttaacatgac
      781 atgaccatcc acccaaaagg cacctcaccg gtgcctttac catttctggg ttgtatatct
      841 gcattcagtt aattactctg cttaaaccat gagagcagag agaaaaaaat gaaatacgat
      901 ctcatcatta ttggcagcgg ttccgtaggc gctgccgccg ggtattatgc aacccgcgcc
      961 ggtttaaacg tgctaatgac cgacgcccat atgccaccgc atcaacacgg cagccaccat
     1021 ggcgatacgc gattaattcg ccatgcttat ggtgaaggcg aaaagtatgt cccgctggtc
     1081 ctccgcgcgc aaacgctgtg ggatgaactt tcccgccaca acgaagatga tcccattttt
     1141 gtacgctctg gtgtcattaa cctcggcccg gctgactccg catttctcgc caacgtcgcc
     1201 cacagcgccg aacagtggca actcaacgtt gaaaagctcg atgcgcaagg gattatggcc
     1261 cgctggccag aaatacgcgt cccggacaac tacatcggct tatttgagac tgattccggt
     1321 tttttgcgca gcgaactggc gattaaaacc tggatccaac tggcgaagga agcgggctgt
     1381 gcgcaactgt tcaactgccc ggtcaccgta attcgtcatg acgatgatgg cgtaactatt
     1441 gaaacggttg acggtgagta tcaggcgaaa aaagcgattg tctgcgcggg aacatgggta
     1501 aaagacctgc tcccggagct gcctgtccag cccgtacgta aagtatttgc ctggtatcag
     1561 gccgatggcc gctatagcgt gaagaataaa ttcccggcgt ttaccggtga actgcccaat
     1621 ggcgatcaat attatggttt tccggcagaa aacgacgcgt tgaagattgg caaacataac
     1681 ggaggccagg ttatccattc agcggatgaa cgtgttccgt ttgcggaagt ggtcagcgat
     1741 ggttcggaag ccttcccgtt cttgcgcaat gtattgccgg gtatcggttg ctgcctgtac
     1801 ggcgctgcct gcacctatga taattcgcct gacgaagatt ttattatcga taccctaccc
     1861 ggccacgata atacactgct cattaccggc ctgagtgggc acggttttaa atttgcgtca
     1921 gttttagggg aaatagctgc cgattttgcg caagacaaaa aaagcgattt tgatttgacg
     1981 ccattcaggc tttcccgctt ccaataatca tcattacggc ctccagaatg aggccgtttt
     2041 tacaggagta tttaatgcgt cggctgttgc actatctcat caataatatt cgcgagcatc
     2101 tgatgcttta tctttttctc tggggattgt tggcaattat ggatctgatc tatgtgtttt
     2161 atttttagtt ttccgcactg acataaactg acaaatattt aatctccctt tcatttggcg
     2221 tgaaaaattg aatgagatta ttcctgcttc tacagcactc accagacatc tctgatgctg
     2281 aacccactaa acttatgaag attctttcat tattaattca aaattattga aataaaaata
     2341 aatatcattc aatttgttat ttttgtgttg caattcattc tccgacacgc cattttcttt
     2401 ctctcgcaaa ttcgtattgg atgcacttat gtcattcaca aatacccctg aacgctatgg
     2461 tgttatatct gcggccttcc attggctaag cgcaattatt gtctacggca tgtttgcttt
     2521 aggtctgtgg atggtcacgc tcagttatta cgatggctgg tatcacaaag cacccgaact
     2581 gcataaaagc atcggtattt tgttaatgat ggggctggtt attcgcgttc tgtggcgtgt
     2641 catatctcca ccgccgggtc cgctgccgag ttattcgccc atgactcgtc ttgccgcaaa
     2701 ggctggacat ctcgcattgt acctattgct gttcgccatc ggcatcagcg gctatctgat
     2761 ctcaactgcc gatggtaaac cgatcagcgt ttttggctgg tttgacgtcc ccgcgaccct
     2821 tgccgacgct ggcgcacagg cagactttgc cggtgccctg catttttggc tcgcgtggag
     2881 cgtcgtagta ctgtccgtta tgcacggatt tatggccctg aagcatcatt tcatcgataa
     2941 agacgacact ctgaagcgca tgctgggaaa gtcgtcatct gactatggag tataaaaaat
     3001 gaaaaaaagc ctgcttggtt taaccttcgc gtccctgatg ttctctgccg gttcagcggt
     3061 tgccgccgat tacaaaattg acaaagaagg tcagcacgcc tttgttaact tccgcatcca
     3121 gcatcttggc tatagctggt tatatggcac ctttaaagat ttcgacggta cttttacctt
     3181 tgacgaaaaa aatccggctg ccgataaagt gaatgtgaca attaacacca ccagcgtcga
     3241 tactaatcac gccgaacgcg ataaacatct tcgcagtgca gatttcctca ataccgcaaa
     3301 atatccacag gcaacattca cctccaccag cgtgaagaaa gacggtgacg aactggatat
     3361 taccggcgat ctgacgctga atggcgtaac caaacccgtc acgctggaag cgaaattaat
     3421 tggtcagggc gacgacccat ggggtggtaa acgtgcaggc ttcgaggccg aaggcaaaat
     3481 taagctcaag gacttcaata tcaagacaga tttaggtcca gcttctcagg aagtggatct
     3541 gattatttca gtggaaggcg tacagcagaa gtaagcgtga aatgttgccg gatggcacgc
     3601 atccggcaat gatatttatt ccgttggatc aggaatgccc agtgttgtat tcagacgtcc
     3661 acgagactta ttaaagatct tattgccatt ttcacgtcct gcccgacggc gtcgctgttc
     3721 ttctggcggt aacgcgcttt cttcgcagca aatctcacta caacaacctt tgtatttttc
     3781 cgcacatact ggacactgaa taaacagcag gtggcagcca tcatttttac agttggtatg
     3841 gctgtcgcac ggcgcgccgc actggtggca atgcgcgata atctcatccg atatacgttc
     3901 gcccatccgc tcgtcaaaaa caaaattttt gccaataaaa cgcaccggca agccctgctc
     3961 gcgcgcctta cgggcgtatt caataattcc gccctcgata tgccagactt tgttgaatcc
     4021 gttatgtttc atccaggcac tggccttttc acaacgaatg ccgccggtgc agtacatgac
     4081 gattttttta tctttatgtg cctgcatcat ctcgactgct tttggcagct gctcacggaa
     4141 ggtatctgcc ggaatttcga gcgcgttttc aaagtgcccc acttcatact catagtggtt
     4201 acgcatgtcg ataaatagtg catcgggatc gtcaagcatg gcgttcactt ccgccgcttg
     4261 cagatactcg ccaacattgc tggcatcaaa gtgaggatcg tcaataccgt cggcaacaat
     4321 gcgatcgcgt accttcatgc gcagtaccca gaaagatttc ccgtcatcat ccaacgcgat
     4381 attcaggcgt aaaccctcta aagccgggtc gaaggcatag agctgcgcgc gaaatgtttc
     4441 aacattgctc gcaggtacgc tgatttgcgc gttaatgccc tcatgcgcca gatacactcg
     4501 cccaaaaaca ttcagcgcgg taaacagctg atataaagcg tcacgggtcg ccttaggatc
     4561 ggcgatgtgg aaatacttgt aaaacgaaat ggtggttcgc ggttcgctct cagccaacat
     4621 tttggctttt agcgcgtcgt tggaaatgcg gttgtgtaac actggcatgg tgtacggttc
     4681 ctgcgagatg ggaaagtaaa aatccgcggc atgatatagc aattatcgat aattaacatc
     4741 cacacatttt acgctacatt tgcgcattaa aaattatttg ttatttacaa gtgcggcaat
     4801 ttcgcccagt cttcagccac aaattttggt tgcgggcgaa aaaatgcgac aatacataca
     4861 attgcccgaa taggttgaaa aacaggattg atatgacgaa tctacccaag ttctccaccg
     4921 cactgcttca tccgcgttat tggttaacct ggttgggtat tggcgtactt tggttagtcg
     4981 tgcaattgcc ctacccggtt atctaccgcc tcggttgtgg attaggaaaa ctggcgttac
     5041 gttttatgaa acgacgcgca aaaattgtgc atcgcaacct ggaactgtgc ttcccggaaa
     5101 tgagcgaaca agaacgccgt aaaatggtgg tgaagaattt cgaatccgtt ggcatgggcc
     5161 tgatggaaac cggcatggcg tggttctggc cggaccgccg aatcgcccgc tggacggaag
     5221 tgatcggcat ggaacacatt cgtgacgtgc aggcgcaaaa acgcggcatc ctgttagttg
     5281 gcatccattt tctgacactg gagctgggtg cgcggcagtt tggtatgcag gaaccgggga
     5341 ttggcgttta ccgcccgaac gataatccac tgattgactg gctacaaacc tggggccgtt
     5401 tgcgctcaaa taaatcgatg ctcgaccgca aagatttaaa aggcatgatt aaagccctga
     5461 aaaaaggcga agtggtctgg tacgcaccgg atcatgatta cggcccgcgc tcaagcgttt
     5521 tcgtcccgtt atttgccgtt gagcaggctg cgaccacgac cggaacctgg atgctggcac
     5581 ggatgtccgg cgcatgtctg gtgcccttcg ttccacgccg taagccagat ggcaaagggt
     5641 atcaattgat tatgctgccg ccagagtgtt ctccgccact ggatgatgcc gaaactaccg
     5701 ccgcgtggat gaacaaagtg gtcgaaaaat gcatcatgat ggcaccacag cagtatatgt
     5761 ggttacaccg tcgctttaaa acacgcccgg aaggcgttcc ttcacgctat taaatctccc
     5821 ctgccggatg cttcagaatg gcatccggca ttaccacagc aaatccccct gatttagcga
     5881 taaaagctct ctggattgcg ccccctggaa gtcgagcgca taattagtgt gcttatcttt
     5941 tcttcttatg ttcaccgcgc ctggcgcacc aacagcggat tgctatgtca ccctgtgaaa
     6001 atgacacccc tataaactgg aaacgaaacc tgatcgtcgc ctggctaggc tgttttctta
     6061 ccggtgccgc cttcagtctg gtaatgccct tcttacccct ctacgttgag cagcttggcg
     6121 ttaccggtca ctccgccctg aatatgtggt ccggtattgt cttcagcatt acatttttat
     6181 tttcggccat cgcctcaccg ttttggggtg gactcgccga ccgtaaaggc cgaaaactca
     6241 tgctattacg ctctgccctc ggcatgggca tcgtgatggt gttgatggga ctggcacaaa
     6301 atatctggca gtttttgatc ctacgggcgc ttcttgggtt acttggcgga tttgtcccca
     6361 acgctaatgc tcttatcgcc acacaagtac cgcgtaataa aagcggctgg gcgctgggta
     6421 cgctctccac aggcggcgtt agtggtgcgt tgctcggccc aatggctggc ggcctgctcg
     6481 ccgatagcta cggcttacgt ccggtattct ttattaccgc cagtgtgctc atactctgct
     6541 ttttcgtcac cctgttctgc atcagagaaa aattccagcc ggtcagcaaa aaagagatgc
     6601 tgcacatgcg ggaagtggtg acatcactta aaaacccgaa actggtactc agcctgtttg
     6661 tcaccacgtt aatcatccag gtggcgacag gctcaattgc ccccattctg acgctgtatg
     6721 tccgcgaact ggcgggtaac gtcagtaacg tcgcctttat cagtggcatg atcgcctcgg
     6781 tgccaggcgt ggcggctctg ctaagcgcac cacgactcgg caaacttggc gatcgaatcg
     6841 gacccgaaaa gatcctgatt acagcgctga tcttttctgt actgctgttg atcccaatgt
     6901 cttacgttca gacgccattg caacttggga ttttacgttt tttgctcggt gccgccgatg
     6961 gtgcactact ccccgccgta cagacactgt tggtttacaa ctcgagcaac cagatcgccg
     7021 ggcgtatctt cagctataac caatcgtttc gtgatattgg caacgttacc ggaccattga
     7081 tgggagcagc gatttcagcg aactacggtt tcagagcggt atttctcgtc accgctggcg
     7141 tagtgttatt caacgcagtc tattcatgga acagtctacg tcgtcgtcga ataccccagg
     7201 tatcgaactg atttttcgcc tttcatactt gcaaaagcgg agaatcagct atccttttcc
     7261 ctgaaacctc atcaactcaa agggagaatc gtgatgacca tgtacgcaac gcttgaagaa
     7321 gccattgacg ctgcacgcga agaatttctt gcagacaacc ccggcatcga cgccgaagat
     7381 gcgaatgtgc aacagttcaa tgcccaaaaa tacgttttgc aggacggcga catcatgtgg
     7441 caagttgagt tttttgccga cgaaggggaa gaaggtgaat gtttacctat gcttagcggt
     7501 gaagccgcgc aaagtgtttt tgatggcgac tatgatgaga tagagatacg ccaggagtgg
     7561 caggaagaga atacattaca tgaatgggac gagggggaat ttcagcttga gccaccgctg
     7621 gataccgagg aaggacgcgc agcagctgat gagtgggatg aacgttaatc actcatacgg
     7681 gccatgatga atgtcgatcg gcagcagtaa agtatcaaaa accagagaga atggcagatc
     7741 aaggatcgtg acataacgcc aggcggagtc acgcacatcc cattgcacac cgggataata
     7801 ttggttgcca tgcccctgcc ccggtatagt gcgactaata atgctgccac agccactcag
     7861 caaggtcacc atgatgctca ccacaattaa acgcatttga tacctctttc gtaaaaaaat
     7921 accggcataa cgccggtatt tttattggct cgttgttttt gccggatgcg gcgtgaacgc
     7981 cttatccgac ccacaaaatc ttgcaaaatc aataaattgc aggaacgatg taggcctgat
     8041 aagcgtagcg catcaggcaa ctacgttttt attgcgaagc cgcatccggt ttacgcaatg
     8101 ccagtggatt gagctttatc ccttcgtaat aactcaccca tgaagaatat ctctccgggg
     8161 aattccagac gcggaaatgc agacgggcca tcgtcaccgg atcgcttagc agcaccaggc
     8221 gacgatcgcg attcagcttc tctggcgtct cgttcagcgc ctgttcaacg tggcggtcac
     8281 gggcgatttc cagcacctta ctggcgcggt gacgcgcggt cgccattgcg gttgccagag
     8341 cgttaaatga cgggttaaac actgcgtgca taaagccatc atcaagggag cgttgacgat
     8401 tcatctcaag gaaccgatcg gtatcaacca gcacctgcgg cggcgaatac tcttccggga
     8461 tcaggaacag tttccagcgt ttggtgcgca gaccaacggt ggcacggctg gaaatcaccg
     8521 aaacaaacgg tgacaggatc aacgagaaga caatcggtgc cagccagaac aggaaacgca
     8581 gatccaacca cgccatacca acagcccaca ctaaccctaa cagcagctgt gagccgtggc
     8641 gtttgaacgc ttcaccccag gaagtggagt catcatcacg ctgcggtgaa ttccacacca
     8701 cttcccagcc aaggaacgcg ctgacgacga agaccgtatg gaacagcatg cgtaccggag
     8761 ccagcagcac ggaaaacagc acttccagca gcaacgataa tgtaacgcgc cagaagccgc
     8821 cgtattcttt cgttcctttg caccagataa gcaaaatgct caataacttc ggcaggaaca
     8881 acagcaccat ggtcgaagca aaaagtgcaa tcgccagctc aggacgccac tgcggccaca
     8941 ccgggaacaa ctgccgtggt tgcaggaagt attgcggttc ggtcaacgca tgtactacct
     9001 gcaatgcagt agagagcgcg aggaacataa accacagcgg agcggagaga taagacatca
     9061 cgcccgtcag gaataccgca cggtgaaccg ggtgcatacc cttcaccagg aacagacgga
     9121 agttcatcag gttaccgtgg caccagcggc ggtcacgttt tagctcatca agcaagttag
     9181 gcggcaattc ttcataagaa cccgggagat cgtaagcaat ccagaccccc caacctgcac
     9241 ggcgcatcaa cgccgcttcc acgaagtcat gtgacaggat tgaaccggca aaggaacctt
     9301 cgcccggcag cggagccagt gcgcagtgct cgataaacgg tttcacgcgg ataatcgcgt
     9361 tatgtcccca gtagtgcgac tcgccaagtt gccagaagtg caaaccggct gtaaacagtg
     9421 gcccatacac gcgggtcgcg aactgctgac agcgcgcata cagcgtatcc ataccggacg
     9481 ctttcggcga cgactgaatg atcccggcgt tcgggttggc ttccatcagg cgcaccaggc
     9541 cgcataaaca atcaccggtc attaccgagt cagcatccag caccaccatg tagctgtact
     9601 ggctgcccca gcgacggcag aagtcatcga tattaccgct tttacgcttc acgcggcgac
     9661 ggcggcggcg atagaaaatc tgaccttcgc caccgacttc agcgataagc tccatccagg
     9721 ctttttgctc tgcgacgcag atatccgggt tataactgtc actaagaatg tagacatcaa
     9781 agtgtttggc attcccggtg gcttttactg attcccacgt tgcacgcagg ccagcaaaaa
     9841 cacggttcac gtcttcgtta cagataggca tgatcaacgc cgtgcgatgc tccgggttta
     9901 atggttcatc gccaactgtt gacgcagata tactgtattt atcgcgacca ataagcagtt
     9961 gcaggaagcc cattaacgcc gtccagaatc cggcggacac ccaacagaac agtaccgcaa
    10021 agaggatcag gataccggtt tgcagcatat aaggcagaag ctgcataaag gaaacccaca
    10081 aatcctgacc aaccatatcc ataggattaa tcagcgccca accctgataa ggaagaatgg
    10141 tcttcatata ccaggtcgcg acgacagttt gcgcgagcgt caggatcaac agaatgtaac
    10201 ggcggatggt accgacggta cgccactttt gctcgctctc ctgctcttct ttggtcaaac
    10261 gagccagata gcgcggcgtg acatcgcgtc cacgcaggcg atcccagaaa cggcctaccg
    10321 ggttggtacg ccacgggtcg ggaaacatcg aggagcgttt tgcttctggc atcgccttca
    10381 gctgatcgcg cccttcgtcg tctttaatta actgtccatc agcaagtgaa tctggccagg
    10441 cttgttccag acgcgccttt accgagcctt gcggggaatc atcctcccgc gcccaggtgc
    10501 ggtgttcggc atccagcgcc tgatgaacgg cgcggatatc agtcttcggc aatgccgctt
    10561 tctcgcttgc ggcgatgggc attgcgtcaa tgtactcagt tgtcttattc attggcaggt
    10621 aactggtagc tccaggtttc actcaacgtc tgatcggcat tcaccagcgc agcacgcatt
    10681 tcagtggttt tcttggcatc tttcactttc acacgcatca ccagacgcca gcctttggta
    10741 accgggttat agcgcaccgt gctttcaact atctcaccat tatcaccaat gctggtttgc
    10801 gctgtgaccg gggtatcctc tggcagtttt ttcatctctg cgccggtaaa atcgaccaca
    10861 aaggcgatag taccgtcagg ctggcgaatc aggttcgact gcttcacatc ccccgttgaa
    10921 cgacgcgttt gttgcaccca tgcgttatct ggcgcatgca gtttgtcttc atcacggctg
    10981 aaggtgatgg tgtatttaaa gttcatctct ttacccggct ccggcagctg atccggcgtc
    11041 cagtaagcga cgatgttatc gttggtttca tcgttggttg gaatttccac cagctcaacg
    11101 ctgcctttac cccactcccc tttcggagtc acccatgcgc ttggacgaag atcgtaacga
    11161 tcatcgagat cttcaaagcg ggagaaatca cgaccgcgct gcaacagacc gaagccttgc
    11221 gggttctcca tcgagaagct gctgaccgct aaatgtttcg ggttattcaa cggacgccag
    11281 atccattcgc cattaccggc atggatagac agaccgttag agtcgtgcaa ctccggacga
    11341 tagttatttg caggcgacgg ttggttcggc ccaaacagga acatactggt taacggtgca
    11401 acccccagtt tgccgacttt atcgcgcaga tagattttcg actgcacatc cacaaccgtg
    11461 tcacgccctg gcataaccac gaatttgtaa gcacctgtcg cgcgcggcga gtcaagcaat
    11521 gcataaatgg ttaaacgttt atcagtcggt tttggacgct cgatccagaa ctctttgaag
    11581 cgtggaaatt cttcacccga tggcaaggcg gtatcaattg ccaggccgcg ggcagaaagg
    11641 ccataaacct gacctgcacc aatcacgcgg aaatagctgg ccccgagcat gctgacgatt
    11701 tcatcgtttt tatctttgct gttgatcggg taaagcactt taaaaccggc aaaaccaagg
    11761 tcttttaccg tgtctttgtc atgctgaaca tcgccgaaag tgaaataatc cgggctgtat
    11821 ttgattcgtt tgactgcggt ggcagtcact tcatttattt tgaccggggt atcgaagtac
    11881 ataccctgat ggtagaactc gagtttgaat ggggtcttca gattgttcca gtacgctttg
    11941 tcatgattaa actggatctg ctgatagtcc gcgtatttca tatcgcggaa aacggagggc
    12001 aagttgcttt tgggcgcctc atagcctttc ccggctaagg attgagcttg ctttgcgaca
    12061 tcatcaatac tgaaagccca gcttgaagat gtatacaggg ttaacattac tgcagcactc
    12121 aaccaacgca ttttcatcat ttgtagttta tgtttcataa ttagtaagca cttccccctt
    12181 tgtgtgctta tatcgatccg atctatttta atggataacc agccattccg acaacagaat
    12241 cccggtatcg ttcagcacgc tggctcatgg attaagcacc tgattgcaac cgttttcact
    12301 ttccgtgctt ctcctaaaga cagaagcatc gacttcgtgt agggtttgcc agcaatttta
    12361 tttatcaacc gtcttacaga cgcttccatt ctggttagtg gtagcgatga tgctatgcat
    12421 aaaaaccttg aagcctgcgc ctggcgcggt caggtcggtg tcgctatggc aacaagccta
    12481 aaggcataac ccgacaggga taagacgaaa aatccgagat tagttaacca tatatgaatc
    12541 cagtacccgc gcaacgtgaa tatttcctcg actccatccg cgcctggctg atgttgttag
    12601 ggatcccttt tcatatttct ttaatctatt cgagccatac atggcatgtg aatagcgccg
    12661 aaccatcatt gtggctgacc ctttttaatg acttcatcca ctcgttccgc atgcaggtat
    12721 ttttcgttat atccggctac ttttcctaca tgcttttttt acgctatccc ttgaaaaaat
    12781 ggtggaaagt acgtgtcgaa cgtgtaggta tcccgatgtt aacagccatc cccctactga
    12841 cattaccgca atttattatg ctgcaatatg tcaaaggaaa agcggaaagt tggcctgggc
    12901 tgtcattgta tgacaaatat aatacgttgg cctgggaatt aatatcacac ctgtggtttt
    12961 tactggtgtt agtagtcatg acgacgctgt gcgtatggat atttaagcgc atcagaaata
    13021 atttagaaaa ttctgataaa acgaataaaa aattctcgat ggtaaaacta tcggtgattt
    13081 ttttatgcct cggcatcggt tatgcggtaa taagaagaac gatttttatt gtgtatccgc
    13141 ccattctgag taatggcatg ttcaatttta ttgtcatgca aacgctgttt tatttgccgt
    13201 tctttatcct cggcgcactg gctttcattt tccctcatct taaagccttg tttaccacgc
    13261 cgtctcgtgg ctgtaccctt gcagcagcat tggcgtttgt cgcttattta ctcaaccagc
    13321 gctatggcag tggcgatgcc tggatgtacg aaaccgagtc ggtgatcacc atggtcctcg
    13381 gtctgtggat ggtgaatgtg gtcttctcct ttggccaccg tttgcttaac ttccagtcag
    13441 cgcgggtgac ttattttgtt aacgcatcgc tgtttatcta tctggttcac cacccgttaa
    13501 cgctgttttt cggcgcatac attacaccgc acatcacctc caactggctt ggttttctct
    13561 gtggcctgat atttgtagta gggattgcga taattctgta tgaaattcat ttgcgcatcc
    13621 cgttactgaa gtttttgttc tctggtaaac cggttgttaa gcgtgagaac gataaagcac
    13681 cagcccgtta agccacattt acaataacca ttccacgggc aatatcgacg ccagtctgac
    13741 cataacccgc ttccagaaac tggtggcggg ttcttttttg agaataatct ctttctttgc
    13801 atgacgatca acccagttga tccgtcccca cctgtccaga cggagctgcc aggccgcatc
    13861 atactggctc tgaataaagc gtttatcaat taactgtgcc agcgtttcgc tctctatcac
    13921 gaagcccatt tcagtattga gcaatgttga acgcggatcg aaattgaaag agccgataaa
    13981 caccgtttta ccatcgatgc taaaggtttt agcatgcagg ctggctccgg aattaccggt
    14041 tatgccgcga tcgtgtaacg tactactttg ttcacgcgtc ggcttgagtt catataattc
    14101 cacgccatag cggagcaatt ttttgcgcca gcgcgcgtat ccggcatgga cgacagcaac
    14161 atcgttagcg gcaagagaat tggttaggat cgcaatcttt accccttttc tcaccatccg
    14221 taagagttgc gccacacctg cgcgtgtcgg tacaaaatag gaagagataa tatcgatgcg
    14281 ttcactgggt gagcccatga tatcgaacag gcgctgcggt aacagtgaat gccgttttgc
    14341 cttgccctcc cctttcgccg gatcatcact taataaacgt gttttcgccc agataagcgg
    14401 caacgttcca tcaaccagat gatttataaa cggactggat tccattttgc gtaagtaacg
    14461 atgcgtcata gcatcgttat gccaggaggc gggtaactcg atgcgatccg ccatttcacc
    14521 ttccgggaca tccagcactt gctgtaaggg tgaaaccgat ttgcaatacc agtagcgggc
    14581 gaaatcatcg gcaacgtcct ctaccacggg ccctattgcc ataacatcta aatccgaaaa
    14641 aagtggctct tcccctgccc caaaataggc atcaccaata tttcgccccc ctaccagcgt
    14701 caccacgcca tcgacagtga aacttttatt gtgcatacgg cgattgagac gggaaaagtc
    14761 ggtgatataa ccaagcggac gcagcaagcg aaacgagaaa ggattaaaaa gccggacttc
    14821 aatgcgggga tggctgtcaa gcaagcgtaa aatgtcgtca agtccgggcg tattgttgtc
    14881 gtccagcaac aaacggacgc gaacgccacg ctttgctgcg gctaacaggg cggaaaacag
    14941 taaccgaccc gacatatcgt cctgccagat gtaatactga acatccagcg tatgctctgc
    15001 catttcggcc agacgatacc gggcggcaaa cgcatccaga cttttctcca gagggaaaag
    15061 accacactga ccgggatgtt gcgaacacag tggcagcacc gcgctcgcca gccggggcaa
    15121 atcattcatc tccttgttgg gtaaggagtc tttcgtagag gtgggcgttt tcttcatcat
    15181 aacagacaaa gtatacctgt tcgggtaaag cgtgacgggt aataaattct gaaacggttt
    15241 taactgcgat ttcagccgct gccgcacgag ggtaaccata aaccccagta ctgattgcag
    15301 gaaaagccac tgacgtatag ctgtttgccg ccaccagtcg taggctattg agataggcat
    15361 cctgcaaaag ctggtcttcg ttttgttcac caccacgcca gactggcccg acggtgtgca
    15421 ctacggcttt agcgggaaga gcgcctgcaa gcgtaataac ggcatgcccc gtagggcaat
    15481 cgccctgctg ttgcctgact tttaaacagg catccagcag ggccggaccc gctgcgcgat
    15541 gaatggcccc atcgacgccg ccgcctccca ttaatgacgg attagccgca ttcacaatca
    15601 catcaacggc cagtttggta atatcaccct gcacaacatg aatacgcgtt ttcataacgc
    15661 ctccttacac cttcttgttt cttaagtgta cagcaggctt tttggttaaa tcgcattttt
    15721 ctgctcagca acgtgcgttg ttcagaaact tagttatatt cgatgttcat tacagccagc
    15781 tttttctgtt tatcaagata ctgcactttt actgaggcta tttgcgcgtt tttaacatta
    15841 ccgctcatta acgctttgct ggaataggtt ttgcccggaa cagaaccatt ctgtgggcag
    15901 ttcatttcta cgggtgatga ctgggtgctg acgtcacatg ccggttccac aatttggcca
    15961 taaaaatgga tcaccccccc ttgcgcggtg cttcctgcga tggcaataaa aggaaaaagc
    16021 aaactgccga gcataagaga gtttaaaaaa ggtctgaaca tttattctat ttcctcaatg
    16081 attagtcatc cttgagggtt gtgttatcca tactttcagg ttggctgatt cgcagcagac
    16141 cattctctcc agattcatct tatgctcgat atttcaacaa attaagactt ttctgaagag
    16201 ggcggccatt gttgtgataa atgaagtgac tgtccatcag aaacagtaac aactattttc
    16261 acccgatctt ccggggaaat atttaaactc aacttcgtca aagcaatagg ttgattagca
    16321 ggcaatgaga gggtcttttc ttgcttcgtc tgactttgcc ctgaactgcc ttcgcgcagg
    16381 gacaatattt gtactctgca cagacaagat tgagtaagag tgacttcagg aataatggta
    16441 tacacatccc cttgctgggt cgtattaaag gttatctggc tggaaagtgc cgcaaggagt
    16501 aataacgcat tcatattctt ctcccgaaaa aaaacagggc ttgcgccctg tttctgtaat
    16561 acaaatgatg tattagtact gatgagcggt cgcgttgtta ccaaagccaa cctgagtcac
    16621 gttgacggag gagttagatg cagtctggtc aactgcagca ccgttgccac caccgaactg
    16681 tttaacagtc atttcagaat tttttccgtt ccactgatca agagtggcgc tgttaccgaa
    16741 gccacgttgg gtcagatcga ttgagctgtc atctgagccc tgacctacat ctgcaccatt
    16801 accaccgcca tgctgggtaa tagtcaagtc agagttacgg gcatcagttt gcagagcaag
    16861 tgcagagtta ccgccaccgt actggtaaat gttcagctct gaatttgggc cgctattatt
    16921 accgccacca ccgtggttac cgccgccgcc gtactgagga acaacacctg ccagagcgct
    16981 accggagaat acgattgctg caattgctgc tacttttaaa agtttcatgt aaaaccccca
    17041 tcggattgat ttaaaagtcg aatggaaatt aacgttgtgt cacgcgaata gccatttgcg
    17101 actgtctctg cactacaatt gccgtttttt gagtaccata ctgtgtaata tttgctttat
    17161 taccagaacc tttctggata atcatcgcag tattaccata agcaccttgc gaaatactgg
    17221 catcgttggc actgcccgcc tgatcaatat atgcaaggtt ataatctcct gtctggtcaa
    17281 tctttgcccg gttgctacta ccttcttgcg caacaaccgc caaaagtttt gagcctccct
    17341 gccgtaactg agcactatta ttcgtcccag cttgaccaat tatggctgcc tgattaaatg
    17401 aagacttact caattcattt accgcgaagt tatattctga attagctaaa tcataacctg
    17461 ctgcggctgc aatcccaggc gcacccagta ttgttaacat cataaataac aatttgtttt
    17521 tcatgttgtc accctggacc tggtcgtaca tttaagaaat taaatcattt caacttggtt
    17581 gttaacgcaa cctgtatttt gttaacgctg cgttacgatg gaaagtatgt ctgcggaaat
    17641 atttttaata actcacccgc gcgttgtatt tttctttttt tgttcatacc aaagtattaa
    17701 aacccacgta aatacgctgt tatctacgca aaaaatattt tgttttcttt taaatctccg
    17761 ttttccgcta tcaaaaatca ccagacagtc atttttcttg cccgtcgctg attgctgcga
    17821 tatgtcttgc gcacaagccg tgacaaacct cgtctttttg cagtaaaaaa ttgtccacgg
//
LOCUS       DAZGJW010000090        17860 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZGJW010000090 DAZGJW010000000
VERSION     DAZGJW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.090"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            68..586
                     /gene="yfiR"
                     /locus_tag="ACHKIK_003151"
     CDS             68..586
                     /gene="yfiR"
                     /locus_tag="ACHKIK_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO9084274.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            576..1802
                     /gene="dgcN"
                     /locus_tag="ACHKIK_003152"
     CDS             576..1802
                     /gene="dgcN"
                     /locus_tag="ACHKIK_003152"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO9084275.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAKKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1818..2300
                     /gene="yfiB"
                     /locus_tag="ACHKIK_003153"
     CDS             1818..2300
                     /gene="yfiB"
                     /locus_tag="ACHKIK_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO9084276.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2377..2724)
                     /gene="rplS"
                     /locus_tag="ACHKIK_003154"
     CDS             complement(2377..2724)
                     /gene="rplS"
                     /locus_tag="ACHKIK_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO9084277.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2766..3533)
                     /gene="trmD"
                     /locus_tag="ACHKIK_003155"
     CDS             complement(2766..3533)
                     /gene="trmD"
                     /locus_tag="ACHKIK_003155"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO9084278.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3564..4112)
                     /gene="rimM"
                     /locus_tag="ACHKIK_003156"
     CDS             complement(3564..4112)
                     /gene="rimM"
                     /locus_tag="ACHKIK_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO9084279.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4131..4379)
                     /gene="rpsP"
                     /locus_tag="ACHKIK_003157"
     CDS             complement(4131..4379)
                     /gene="rpsP"
                     /locus_tag="ACHKIK_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO9084280.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4628..5989)
                     /gene="ffh"
                     /locus_tag="ACHKIK_003158"
     CDS             complement(4628..5989)
                     /gene="ffh"
                     /locus_tag="ACHKIK_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO9084281.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTIKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6156..6947
                     /gene="ypjD"
                     /locus_tag="ACHKIK_003159"
     CDS             6156..6947
                     /gene="ypjD"
                     /locus_tag="ACHKIK_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO9084282.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6968..8254
                     /gene="yfjD"
                     /locus_tag="ACHKIK_003160"
     CDS             6968..8254
                     /gene="yfjD"
                     /locus_tag="ACHKIK_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO9084283.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8309..8902)
                     /gene="grpE"
                     /locus_tag="ACHKIK_003161"
     CDS             complement(8309..8902)
                     /gene="grpE"
                     /locus_tag="ACHKIK_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO9084284.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            9025..9903
                     /gene="nadK"
                     /locus_tag="ACHKIK_003162"
     CDS             9025..9903
                     /gene="nadK"
                     /locus_tag="ACHKIK_003162"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO9084285.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9989..11650
                     /gene="recN"
                     /locus_tag="ACHKIK_003163"
     CDS             9989..11650
                     /gene="recN"
                     /locus_tag="ACHKIK_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO9084286.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11799..12140
                     /gene="bamE"
                     /locus_tag="ACHKIK_003164"
     CDS             11799..12140
                     /gene="bamE"
                     /locus_tag="ACHKIK_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO9084287.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12202..12492)
                     /gene="yfjF"
                     /locus_tag="ACHKIK_003165"
     CDS             complement(12202..12492)
                     /gene="yfjF"
                     /locus_tag="ACHKIK_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO9084288.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12482..12919)
                     /gene="ratA"
                     /locus_tag="ACHKIK_003166"
     CDS             complement(12482..12919)
                     /gene="ratA"
                     /locus_tag="ACHKIK_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO9084289.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            13090..13572
                     /gene="smpB"
                     /locus_tag="ACHKIK_003167"
     CDS             13090..13572
                     /gene="smpB"
                     /locus_tag="ACHKIK_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO9084290.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13787..14149
                     /gene="ssrA"
                     /locus_tag="ACHKIK_003168"
     tmRNA           13787..14149
                     /gene="ssrA"
                     /locus_tag="ACHKIK_003168"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14420..14668
                     /locus_tag="ACHKIK_003169"
                     /pseudo
     CDS             14420..14668
                     /locus_tag="ACHKIK_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
     gene            15326..16687
                     /gene="espK"
                     /locus_tag="ACHKIK_003170"
     CDS             15326..16687
                     /gene="espK"
                     /locus_tag="ACHKIK_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFO9084291.1"
                     /translation="MLPTSQLRPSGTFYSNSAETSAGIKSEITPTQIEEARAIGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDIPSTVKN
                     LHIELTDSPFIHIISEGIECLTVCHCHISGVPESVRHLEIRGYATDGIKNVPNGLLSL
                     SINRYTPENQARIDSLISPSLQTLSLTGCSNIILPEKLPESLRSVTIHVEQKTTWNIV
                     AEGVPDGLDLDLQNVLLSPNVIKEKNITFQGNALDAALHFRPGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIVIQNTLTNAVWDARTPRKYKQDSLIKRALNEHERGTKFKQCLKNH
                     NNYNVIMADLSIYNRDKLWAKTSKAGLEFQTLIRNRTVMFCADGLVNSLKLIANKSEG
                     YGQSITASELRWIYRNKDNSQIMKNIKFYLHGKEIPAERILDTPEWKDYRPKYSGSTY
                     KYS"
     gene            complement(17064..>17860)
                     /locus_tag="ACHKIK_003171"
     CDS             complement(17064..>17860)
                     /locus_tag="ACHKIK_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO9084292.1"
                     /translation="KVGGGFYRYIESFNPISSETPNKIVYKGESVDLTKEPNSELYSG
                     RYSINNKQVNVYFFRDADGTFYKSEGLHGGGVIRYIDKPYSQLREGDIGYDEDLLDIY
                     DDSPVLEDTLPALSSEIVPTPEHSIKQIYSKIKEGHIELSDSDIILCRGTTGIQAENI
                     VEYKTAGGLPDSNPNVKAPDEYMAQQQVRIGRILPEYTSDLSVADRFSREHYLIVVKV
                     KAKYITRGSVTESGWVIDKTAPVEPLAIIDRTFGMKENISMVNASK"
BASE COUNT         4760 a         4553 c         4021 g         4526 t
ORIGIN      
        1 tgatatttta atgagcattc tgaaaaaata tcgatgcatt tcgagcgaag atggtgggga
       61 tccctgaatg cgtttttctc accgactgtt cttacttctg attctcctgc tgacgggagc
      121 accaatactc gctcaggagc cttctgacgt tgcgaaaaac gtgcgtatga tggtttccgg
      181 tattgtcagt tacacccgct ggcctgcgct atcagggcca ccaaagttat gtatcttttc
      241 atcttcacgt tttagtaccg ccctacaaga gaatgccgcg acatctttgc cttatctgcc
      301 tgtcatcatt cacactcaac aagaggcgat gatttcaggc tgcaatggtt tttattttgg
      361 caacgagtca ccgacattcc agatggaatt aaccgaacaa tatccgtcaa aggcgttatt
      421 attaattgcc gaacaaaata ccgagtgcat tattggtagc gccttttgtc tgatcatcca
      481 caataatgac gtcagatttg ccgtaaacct ggatgcctta tcgcgtagcg gtgtaaaggt
      541 caacccggat gtcttaatgc tcgcacggaa gaaaaatgat ggataacgat aattctctta
      601 ataagcgccc cacgtttaaa agagcattac gcaacatcag tatgaccagc atatttatca
      661 ctatgatgct gatctggttg ctgctttccg tgacctcggt gctgaccctg aaacagtacg
      721 cgcaaaaaaa cctggcactg acagcagcaa caatgactta cagtctggaa gcagctgtcg
      781 tttttgccga tggccctgca gcaactgaaa cactggcagc gctgggccag caagggcaat
      841 tttcaactgc agaagtacgt gataagcagc aaaatattct ggcgtcctgg cattacaccc
      901 gtaaggatcc aggcgatact ttcagcaatt tcataagcca ctggctcttc cccgccccca
      961 tcattcagcc gattcgtcac aatggtgaaa ccattggcga agtacgctta accgctcgcg
     1021 acagttcaat cagccatttt atctggtttt cgctcgccgt actgaccggt tgtattctgc
     1081 tggcatcagg catcgcaatt accctcaccc gccatttgca caatggcctg gtagaagcgc
     1141 tgaaaaatat caccgatgtc gtacatgatg tgcgttccaa ccgcaatttt tcccgacgag
     1201 tttcggaaga acgtatcgct gagtttcacc gcttcgctct cgacttcaac agtctgctgg
     1261 atgaaatgga agagtggcag cttcgtttac aggccaaaaa tgcgcagctt ctgcgtaccg
     1321 cgctacatga cccattaacc gggctggcta accgcgcagc gtttcgtagc ggcatcaata
     1381 cgttgatgaa caattccgat gcccgaaaaa cgtcggcgtt actatttctt gatggcgata
     1441 atttcaaata tatcaatgat acctggggtc atgcgacggg cgatagagtc ttgattgaaa
     1501 tcgcaaaacg gttagctgaa tttggcgggc tgcgacataa agcataccgc ctgggcggcg
     1561 atgaattcgc tatggtgctc tatgatgtac agtcggaatc tgaagtgcag cagatatgct
     1621 cagcactgac acaaatcttt aatctcccgt ttgatcttca taatggccat cagaccacca
     1681 tgacattaag cattggttac gcgatgacca ttgagcacgc ctctgcgaaa aaattacaag
     1741 agcttgccga tcacaatatg tatcaggcca aacaccagcg tgccgaaaag ctggtgagat
     1801 aacaaggata tatcgatatg ataaagcacc tggtagcacc cctgattttc acctcactaa
     1861 tactgactgg ctgccagtcc cctcagggaa agtttactcc tgagcaagtc gccgctatgc
     1921 aatcttatgg atttactgaa tccgccggcg actggtcgct gggcttatca gatgccattc
     1981 tgttcgcaaa aaatgactac aaattgctcc cggaaagcca gcaacagatc caaaccatgg
     2041 cagccaaatt ggcctcgaca gggctaacac atgcccgtat ggatggacac accgataact
     2101 atggtgaaga cagttacaac gaaggcttat cattgaaacg ggcgaatgtc gtggccgatg
     2161 catgggctat gggtggacaa attccgcgca gcaatctcac cacacagggt ttaggaaaaa
     2221 aatatcccat agccagtaac aagaccgccc agggccgcgc cgagaaccgc cgcgtcgcag
     2281 tggtgattac taccccttaa aacaccagca aacagataaa aaaagggcca gccaattggc
     2341 cagcccttct taacaggatg tcgcttaagc gaaatcttag ttaagacgct ctttgatacg
     2401 agcagcctta ccagtacgct cacgcaggta gtacagttta gctttacgaa cagcaccacg
     2461 acgtttgaca gaaatgctgt caactaccgg agagtgagtc tggaagacac gctcaacgcc
     2521 ttcgccgttg gaaattttac gaacagtgaa tgcagagtgc agaccgcggt tacgaatagc
     2581 gataaccacg ccctcgaatg cctgcagacg ttttttggaa ccttcaacaa cccatacttt
     2641 cacttccacg gtatcacccg gacggaagga aggtacgtcc tgcttcatct gctcttgttc
     2701 aagttgctta ataatgttgc tcataattta atctcttatc ctgggtaaac tgatatctcg
     2761 ggggcttacg ccatcccatc atgtttatgt tgctgttgtg cgtgttccgt tttgaactcc
     2821 gccagcaacc ttgcttgctc ttcagtcaga gccaggtttt ccagaagttc aggtcttcta
     2881 agccaggtac ggcccagcga ctgtttcaaa cgccagcgac gtatctcggc atggttgccc
     2941 gacagtaaca ctggcggaac ttccatccct tctaacacct caggccgcgt atagtgcggg
     3001 caatccagca atccttcagc aaaggaatct tccgttgccg aggcttcatg tcccagtact
     3061 cccggaataa accgggaaac ggagtcaatc agcgtcattg ctggtaactc accaccactg
     3121 agaacgtaat cgccgattga ccattcttcg tcaatttcgg tttggatcac gcgctcatct
     3181 ataccttcgt agcgaccgca caccagaatc aatttttgat tcgttgccag ttcgctgacg
     3241 cccgcttgat caagcttgcg tccctgtggt gacagataaa tcacctttgc gccttcaccc
     3301 gccgcggctt ttgctgcatg aatggcgtcc cgcaagggtt gcaccatcat taacatcccc
     3361 ggtccgccgc cgtaaggacg atcgtccacg gtacggtgcc ggtcatgcgt gaagtcgcga
     3421 ggactccaac tctggatgct cagcaggcca tttttaactg cccggccagt taccccgtaa
     3481 tcggtaattg cgcggaacat ttcaggaaac aggctaatta tgccaatcca catagcgccg
     3541 tcttttaccg tttatccggt ggtttaaaaa ccaggatccc aatctacttc gattgaacga
     3601 gtagtgagat cgactttctt gataacctgc ccatcgagga acggtacgag acgttccttg
     3661 ataccaaacg catctttcag gtttgcctta atgacgagaa cgtcattaga tccggtttcc
     3721 atcatatcga cgactttacc gagatcgtag ccttcagtgg ttactacctg gcagcccatc
     3781 aggtctttcc agtagtagtc cccctcttca agctgaggca gctgcgatga atccacgaca
     3841 atttcacaat tcgtcagcag gttcgccgca tcacgatcgt caacgccttt cagcttgatg
     3901 atcatgtcct gattgtggtg cttccagctt tccagctgga cttgctgcca ctgacccgcc
     3961 ttctggataa accagggctg atagtcaaaa atgctttcgg cgtcttcggt ggaagaaaac
     4021 actctgagcc acccacgaat accgtaagac gaacccattt ttcccaaaac gatgggatca
     4081 acaggtgctt gcgcggtgag ttgtttgctc atcatgacca ccgtgacaga ttaagctgct
     4141 ttgtttactt ctttgatcag cgcagcaacg cgatcagaaa tagttgcgcc ctggccaacc
     4201 cagtgagcga tgcgatccag atccaggcga gtgccttctt ctttttcgct agcgattggg
     4261 ttgaagaaac caacgcgctc gatgaagcga ccgttgcgtg cattacggct gtcagcgaca
     4321 acaacctggt agaacggacg ctttttagcg ccgtgacgtg ctaaacgaat agttaccata
     4381 acatcctctt gtgtgaataa aacaaccgga ccccatcgag gaacggagtc cggtgtcata
     4441 ttaaaagccc gaaaatttta ctcatttttg cgggaattgc aatcaacagt tgctaactct
     4501 gctgtaaaag gccgtcggcg gtgcagccag tttggtgccg gagtgcgcgc agtcaccgga
     4561 gcgtacacgc agtacgtgag gatgacgagc acatcccggt gccaaaatgg caaacaagcc
     4621 aggccgatta gcgaccaggg aagcctgggg gcatcatacc cttcatgctt ctcatcatct
     4681 tcgccattcc gcctttcttc attttcttca tcatgcgctg catgtcgtcg aactgcttca
     4741 gaaggcggtt cacgtcctgc acctgcatac cgcaaccggc agcgatacga cgcttacgcg
     4801 aacctttgat gatttctggc ttagcgcgct cttttatcgt catcgagttg atgatggctt
     4861 ccatacgcac cagcacttta tcgtccatct gcgacttaac attatccggg atctgcccca
     4921 tgcccggcag cttgcccatc aggctagcca tgccgcccat atttttcatc tggcgcagtt
     4981 gctcaagaaa atcgttgaga tcgaagccgt cgcctttttt cagcttgctg gctaatttct
     5041 ctgcctgcgc gcggtcaact ttgctttcga tatcttcgat cagcgacagt acgtcgccca
     5101 tgccgagaat acgcgacgcg atgcggtccg gatggaacgg ctccagcgcc tcagttttct
     5161 cgccaacacc gagaaacttg atcggtttgc cagtgatgtg acgaatagag agcgccgcac
     5221 cgccgcgggc atcgccgtcc actttggtca acactacgcc ggtaagcggt aacgcttcat
     5281 tgaatgcttt tgccgtattg gccgcatcct gaccggtcat ggcgtcaacc acaaacaggg
     5341 tttcaaccgg gttaatcgac gcatggactt gtttgatctc gtccatcatc gcttcgtcaa
     5401 cgtgcagacg accagcggta tccaccagca gcacgtcgta gaatttcagt ttggcttctt
     5461 tcagcgccgc gttaacgata tctaccggct tctgaccaac atcagaaggg aagaaatcaa
     5521 cgcccacctg ctctgccagc gtctcaagct gtttgattgc cgccgggcga taaacgtcgg
     5581 cagaaaccac cagcactttc ttcttgtgct tctcgcgcag gaacttaccg agtttaccaa
     5641 cgctggttgt tttaccggca ccttgcaggc ccgccatcag tacgaccgca ggcggttgcg
     5701 cagccaggtt cagggtctgg ttctcttcgc ccatcgccgc aaccagttcg ttacggacta
     5761 ttttgacgaa ctcctgcccc ggcgtcaggc tcttattaac ttcatgacca accgctttct
     5821 cttttacgcg attgataaac tcacgcacta ccggcagagc gacgtccgcc tccagcagcg
     5881 ccatgcgcac ttcgcgcagc gtatctttta cgttgtcttc agtgaggcgt ccacggccac
     5941 tgatattgcg cagcgtgcgc gacaaacgat cggttaaatt atcaaacatt gtctctcgcc
     6001 tggggtggaa acggttggcc gcaatcgcga cacatcatca gtattttgcc gcagtataac
     6061 atgaaggcgt ctttgttgtt atgcaacggt tggagcagcg ttcacctgac gctatactgc
     6121 ttctctttct tattgctcaa actgtcgaca ttactatgcc cgtttttgct ctgctcgcgc
     6181 ttgtcgccta ctccgtcagt cttgcgctga ttgttcccgg tctgctgcaa aaaaacggcg
     6241 gctggcggcg catggctatt atttctgcgg tcattgcgct ggtctgccac gcaatcgctc
     6301 tggaagcccg catcctgccc gacggtgata gcggacaaaa cctcagcctg ctgaacgttg
     6361 gttcattggt cagtttgatg atctgtacgg taatgaccat tgtggcttct cgcaatcgtg
     6421 gctggttgct gctacccatt gtctatgcct ttgcgcttat caacctggcg ctggcgacct
     6481 tcatgcccaa tgaatacatc acccatctgg aagcaacgcc tgggatgctg gtgcacattg
     6541 gcttatcgct cttttcctat gccacgctga taattgccgc cctgtacgcg ctacaactgg
     6601 cgtggattga ttaccaactg aagaacaaga agctggcgtt taaccaagaa atgccgccat
     6661 tgatgagtat cgagcgtaaa atgttccaca tcacgcagat tggcgtggtg ctactaacgc
     6721 tcacgctttg cactggcctg ttctacatgc acaacttatt tagcatggaa aatatcgaca
     6781 aggccgtgct ctctatcgtg gcgtggtttg tctatattgt gctgctgtgg ggacattatc
     6841 atgaaggatg gcgtggacgc cgcgtcgtct ggtttaacgt tgcgggtgca gtcatcctga
     6901 cactggccta cttcggcagc cgaattgtcc agcagttaat cagctaaacc cagaaaagga
     6961 gtttcccctg gaacacattt ctactactac gttgatcatt attctgatca tcatggtggt
     7021 catttcagcc tatttttccg ggtccgaaac cggaatgatg accctcaacc gctatcgtct
     7081 gcgacatatg gcgaaacagg gtaatcgctc ggccaaacgc gtcgaaaaat tgctgcgtaa
     7141 gccagaccgc ctgataagcc tggtgttaat cggcaataac ctggtcaata ttcttgcctc
     7201 cgcgctcggc actattgttg ggatgcgttt gtacggcgat gcgggcgtgg caattgcgac
     7261 tggtgtgctg acttttgtcg tactggtttt tgctgaggta ttgccgaaaa ccattgccgc
     7321 gctgtacccg gaaaaagtcg cttatccgag tagttttctg ctggctccgc tgcaaatttt
     7381 gatgatgccg ctggtctggt tgctgaatgc tatcacccgt atgctgatgc gcatgatggg
     7441 tatcaaaacc gatatcgtgg ttagcggctc tttgagcaaa gaagagttgc gcactatcgt
     7501 gcacgaatca cgctcacaaa tttcccgtcg caatcaggat atgctactgt ctgtgctcga
     7561 tctggaaaag atgaccgttg atgacatcat ggtgccgcgc agtgaaatta tcggtattga
     7621 tatcaacgat gactggaaat cgattctgcg ccaactctcc cactcacctc acgggcgcat
     7681 cgtgctctac cgtgattcgc tggacgacgc catcagtatg ctgcgagtac gtgaagcctg
     7741 gcggttgatg tcggagaaaa aagagttcac caaagaaacc atgctgcgcg ccgcggacga
     7801 gatctatttt gtgccggaag gtacgccgct cagcacgcag ttggtaaagt ttcagcgcaa
     7861 caaaaagaaa gtcggcctgg tcgtcaacga gtatggagac attcaggggc tggtgacggt
     7921 tgaagatatt ctggaagaga ttgtcggcga tttcactacg tcgatgtcgc caacacttgc
     7981 cgaagaggtc acgccgcaaa acgacggttc ggtgattatc gatggcaccg ccaacgtgcg
     8041 ggaaatcaac aaagccttta actggcatct accggaagat gatgcccgca cggttaatgg
     8101 cgtcattctt gaggcactgg aagagatccc tgtcgcaggc acccgcgtgc gtattggcga
     8161 gtacgatata gatattctcg acgtacagga caatatgatt aagcaggtaa aagtttttcc
     8221 tgtgaaaccg ctgcgcgaga gtgtggcgga gtaacgaaaa cggccctgca ttcgcatgca
     8281 gggccgtgaa ttattacgaa aacagaaatt acgcttttgc tttcgctaca gttaccatcg
     8341 ccgcacgaat cgtacgacca ttcagcgtat aacccttctg cataatgccc agtacgttac
     8401 ctggcgcaac gtcatcagat tccaccattg cgatagcctg atgcacattc gggtccagcg
     8461 gcacgttagt ttcggcgatc acttcaacac caaacttacg cacaacatcc agcatcgact
     8521 tcagcgtcag ctcaatgcct tcaaccatcg cagacatatc cgggttagct ttatcagcca
     8581 cttccagcgc acgatccagg ctatcaatca ccggcagcaa ttcgttgatg aatttctcca
     8641 gcgcgaattt gtgggctttt tcaatatcca gttcagtacg tcgacgcagg ttttccattt
     8701 cggcttttac acgcaaaatg ccgtcacgtt cacgggtctg ggcttcagcc agctgagctt
     8761 cgagattcgc aattttttca tcgcgcggat ccacctgctc agcagaagct tctggctcaa
     8821 ctgcctcaat ctcttcgtgc tgatccatga taatttcttc cggggcttgc ccctcaggcg
     8881 ttttctgttc tttactactc atgaatttct ccgcgttttt ttcgcattca tctcgctaac
     8941 ttcgcttatt atggggatca gtttcagggt ttcaagggaa gcactcacat tgtcatcaat
     9001 cttcgcaaca aggacctcgg aaaaatgaat aatcatttca agtgtattgg cattgtggga
     9061 cacccacggc accccactgc actgacaaca catgaaatgc tctaccgctg gctgtgcaca
     9121 aaaggttacg aggtcatcgt tgagcaacaa atcgctcacg aactgcaact gaagaatgtg
     9181 aaaactggca cgctcgcgga gattgggcaa ctagctgatc tcgcggtagt cgttggtggc
     9241 gacggtaata tgctgggcgc ggcacgcaca ctcgcccgtt acgatattaa agttattgga
     9301 atcaaccgtg gcaacctggg tttcctgact gaccttgacc ccgataacgc ccagcaacag
     9361 ttagccgatg tgctggaagg ccactacatc agcgagaaac gttttttgct ggaagcgcaa
     9421 gtctgtcagc aagattgcca gaaacgcatc agcaccgcga taaatgaagt ggtgcttcat
     9481 ccaggcaaag tggcgcatat gattgagttc gaagtgtata tcgacgagat ctttgcgttt
     9541 tctcagcgat ctgatggact aattatttcg acgccaacag gctccaccgc ctattccctc
     9601 tctgcaggcg gtcctattct gaccccctct ctggatgcga ttaccctggt gcccatgttc
     9661 ccgcatacgt tgtcagcacg accactggtc ataaacagca gcagcacgat ccgtctgcgt
     9721 ttttcgcatc gccgtaacga cctggaaatc agttgcgaca gccagatagc actgccgatt
     9781 caggaaggtg aagatgtcct gattcgtcgc tgtgattacc atctgaatct gattcatccg
     9841 aaagattaca gttatttcaa cacattaagc accaagctcg gctggtcaaa aaaattattc
     9901 taattttacg ccagcctctt tactgtatat aaaaccagtt tatactgtac acaataacag
     9961 taatggtttt tcatacagga aaacgactat gttggcacaa ctgaccatca gcaactttgc
    10021 tatcgttcgt gagcttgaga ttgattttca tagcggcatg accgtaataa ctggcgagac
    10081 cggcgcgggt aaatctattg caatagatgc cctcggtctt tgtctcggtg gtcgcgctga
    10141 agccgacatg gtgcgtaccg gcgctgctcg cgctgacctg tgcgcccgtt tttctctgaa
    10201 agatacgcca gcggctctgc gctggctgga agaaaatcag cttgaagacg ggcatgaatg
    10261 tttgcttcgt cgagtgatca gcagcgatgg tcgctcccgt ggtttcatca acggtacagc
    10321 tgttcctctg tcacaactgc gcgaactggg tcagttgctg attcagatcc atggtcagca
    10381 cgctcatcaa ttactcacca aacctgagca ccaaaaattc ctgcttgatg gctatgccaa
    10441 tgaaacctct ctactgcagg aaatgaccgc acgttatcag ttgtggcatc aaagctgccg
    10501 tgacctcgcg catcatcaac agttaagtca ggaacgcgcc gcccgtgcgg aactgctgca
    10561 ataccaatta aaagaactta acgaatttaa tccgcagccc ggagagtttg aacaaatcga
    10621 cgaagagtac aaacgtctgg cgaacagcgg tcaattgctg accaccagcc agaatgcatt
    10681 ggcattaatg gccgacggtg aagacgcaaa cctgcaaagt cagctttaca cggctaaaca
    10741 actggtgagc gaattgattg gcatggacag caaactgtcc ggcgtacttg atatgctgga
    10801 agaagctacc atccagattg ctgaagccag cgatgaactg cgccactact gcgatcgtct
    10861 ggatctcgat cccaaccgac tatttgaact tgaacagcgc atctcaaaac agatttcgct
    10921 ggcacgtaaa catcacgtca gccctgaggc attgccacag tattaccagt cgctactgga
    10981 agaacagcag caactggacg atcaggccga ctcacaagaa acgcttgcgc tggcggtaac
    11041 gaaacatcat cagcaggcac tggaaatcgc gcgcgcatta caccaacaac gccagcaata
    11101 tgcagaagaa cttgcacagc tgatcaccga cagtatgcat gcgctctcaa tgccgcatgg
    11161 gcagtttacg atcgatgtta aatttgacga gcatcacctg ggcgctgacg gtgccgatcg
    11221 tattgagttt cgggtaacca ccaacccagg tcagccaatg cagcctattg ccaaagtcgc
    11281 atccggtggt gaattgtccc gcatcgcact ggcaatccag gtcatcacgg cgcgtaaaat
    11341 ggaaaccccg gcactgattt ttgatgaagt ggatgtaggg attagcggtc caacagcggc
    11401 agttgtcggc aaactgctgc gtcaactcgg cgaatcaact caggtgatgt gtgttaccca
    11461 cctgccacaa gtcgcgggat gtggtcatca acactatttt gtcagcaaag aaaccgatgg
    11521 tgcgatgaca gaaacgcata tgcaatccct gaataaaaaa gcgcggttac aagagctggc
    11581 gcgcctgctt ggtggcagtg aagtcacacg taatacactg gcgaatgcga aagaactgct
    11641 tgcagcgtaa acttttttcc tgcttcacgg tcagagtaaa cagcaaaacg ccgtaagacc
    11701 ggaaagcaaa aggttttaaa gtgatgaaag gtctattatc atcggcatat tacagatgag
    11761 ccacgtactg ctcgggcccg aaaaggaatc aaatcactat gcgctgtaaa acgctgactg
    11821 ctgcagcagc agtactattg atgttgaccg caggctgttc cactctggag cgagtggttt
    11881 accgtcctga catcaaccag gggaactatc tgaccgctaa cgacgtatcc aaaatacgtg
    11941 ttggcatgac gcaacaacaa gttgcgtacg cattgggtac accgctgatg tccgatccat
    12001 ttggtacgaa tacctggttc tatgtcttcc gccagcaacc aggtcatgaa ggtgtaacgc
    12061 agcaaacgct gacgctgacc tttaacagta gcggtgtgtt gaccaatatt gataacaaac
    12121 ctgcgctgag tggtaactaa taataaagtt gctctcaaag acgttaaaaa aggtgctcaa
    12181 tgagcacctt ttttctgtct gttatttatt cgctgatttt tctgctcgtt gcctgcgaag
    12241 ctctttcgga tcggcaatga gagggcgata aatctccacc cgatcgccat catgcacact
    12301 atcgcttagt tttgccggac ggctgtaaat gccgactttg tttttagtta aatcgatatc
    12361 ggtacgcaat tccagcaagc cactggcgcg aatagcttct tcaaccgtcg cgccctcctg
    12421 cagcgtcacg cgctgcaggt actgcttctc aggtagcgca taagccacct caacggcaat
    12481 tttacctggc actgtaaacc tctttcgcac gaaccgtaaa agcctggacc atattagacg
    12541 ccagctcttt aaacacgcga ccaaaggcga gttcaatcaa cttattggta aactcaaagt
    12601 cgagatgaaa ctcgatacga cacgcctcct ggctcagcgg cgtaaacttc catccaccaa
    12661 tcaatttctt gaacggccca tccaccagat tcataagaat actttggtta ctggtcaact
    12721 ggttgcgggt agtaaacgtt ttgctgatcc cagccttaga gacatctacc gccgcagtca
    12781 tctgcccagg agtggactcc agaatccgac ttccggtaca acccggcaaa aactgaggat
    12841 aagactgaac gtcattcact aactgataca tttgctccgc gctgtagggt accagtgcgg
    12901 tccggctaat ctgaggcata acaatttcca tcaacaaaaa tccaacaaat aatatcattt
    12961 atcctgctgt aaaaaaaacg ctatcccggc gctgggtaac atcgggttca tgctaagata
    13021 gagccttgtc ccccgcagga ttgatatggg gtgttttcga tttcagatta ccgatgattc
    13081 acgacgctta tgacgaagaa aaaagcacat aaacctggtt cagcgaccat cgcgcttaac
    13141 aagcgcgccc gtcacgaata ctttatcgaa gaagagttcg aagcgggact tgccctgcaa
    13201 ggctgggagg ttaaatccct gcgcgcagga aaagccaata tcagcgacag ctacgtcctt
    13261 ctgcgtgacg gagaggcatt tctgtttggc gctaacatca cgccaatggc cgtggcctcc
    13321 acgcatgtgg tgtgcgatcc tacccgtacc cgcaagttac ttctcaacca gcgcgaactg
    13381 gactcattgt acggtcgcgt caatcgagaa ggctataccg tagtggcgct ctccctgtac
    13441 tggaaaaatg cctggtgcaa agtgaaaatc ggcgtcgcca aaggtaaaaa acagcacgat
    13501 aaacgttcag atatcaaaga acgcgaatgg caggtggata aagcacgtat catgaaaaac
    13561 gcccaccgtt aaacctgcac tccaattatt gaccagttcc tcaccgcgcc tccctctccg
    13621 gcggcgcgaa tgaacatctt attggctatc acatccgaca caaatgttgc catcccattg
    13681 cttaatcgaa taaaaatcag gctacatggg tgctaaatct ttaacgataa cgccattgag
    13741 gctggtcatg gcgctcataa atctggtata cttaccttta cacattgggg ctgattctgg
    13801 attcgacggg atttgcgaaa cccaaggtgc atgccgaggg gcggttggcc tcgtaaaaag
    13861 ccgcaaaaaa tagtcgcaaa cgacgaaaac tacgctttag cagcttaata acctgcttag
    13921 agccctctct ccctagcctc cgctcttagg acggggatca agagaggtca aacccaaaag
    13981 agatcgcgtg gaagccctgc ctggggttga agcgttaaaa cttaatcagg ctagtttgtt
    14041 agtggcgtgt ccgtccgcag ctggcaagcg aatgtaaaga ctgactaagc atgtagtacc
    14101 gaggatgtag gaatttcgga cgcgggttca actcccgcca gctccaccac ttttgatagg
    14161 actgcaatcg gacagcggca ataaaaacag ccacttacgg actctgacca gacagagcgc
    14221 agaccgagaa aagacaaaaa aatgcacgta aaatgcacgt gcactttaaa agaaccccag
    14281 atctcacggt ctggtttttt tctgtttgta actaatggta acaaaaacac atcacctctt
    14341 ggcgttcctt ctccttgata ctgtttatcc atacagttaa aaataacact gtatacaaac
    14401 acagtataga gagattttta tgcgtattga aatttgcata gccaaagaaa aaatcagcaa
    14461 aatgccaaaa ggatctgttc ctgccctaca gcaagaaatg ccgtgacgtg ccagtaaacg
    14521 ttatgacgat gtagaagtga tcatcaaatc caccagcaac gatggccttt cagttactct
    14581 caccgccgat aaagattctg caaaaacttt tgttcaggag acgctgaaag atacctggga
    14641 gtctgctgac gagtggtttg ttcgctaatt aacgagtaaa atcagtaacg gctggaaatc
    14701 attcaatact cacactatct aaaagttcac cagccaacag cggtaagttc tttcatacga
    14761 cgtgccgcgg atcctattat tctagtatga aatccctaac cgatttcaaa ttctccagac
    14821 tgctcagatt ctcttttatc ccacgctggc gctgataaat ctagtcattg caatcgacct
    14881 gcgctatcgc tgccgcagtt cttccagttt ctgcatcgat aactccatct tctgaataag
    14941 aaggcaacag aatagataat tagtgcttta tattcaaaag gttaatagca aatttggcat
    15001 gctttttact gtctagtaaa cagcatgcct ctatccataa aatcaaacag ttgcaatcta
    15061 attttggaga acgttttttc ttctcccttt tagcaaatcg ctataaaaaa caacaaatgg
    15121 tataaaaatg agcaatccta atccatgcat gacggactgg agaaagtatt cgcaaaagtt
    15181 aaaatcaaca gtctgttgat tttgagatat aactctcttt tctaatattg acaccttggc
    15241 tttgctggtt gctaaaatta gcttgttgat tagcagtatg gatagaactt acactgttat
    15301 ttacttctaa aaatggaatt tatcaatgct tcctacatcg caattacgac cgtccgggac
    15361 attctactcc aattccgcag aaacatcagc aggcatcaaa agcgaaatca caccaactca
    15421 gatagaagaa gcgcgggcca ttggtcgttt atatatcaaa gattgtgata ttgagtatct
    15481 gccacagtta ccaaacgaaa taacatcagt tacaatcgaa aattgcaata acctgaccac
    15541 ccttacagga ttaccggtta atacacaaaa cctgtccgtc attaactgtg aaaaattaca
    15601 aatcacagac ataccatcga cagtaaaaaa tctacatatt gaattaactg attcaccatt
    15661 tatacacatc atatctgaag gcatcgaatg cctgacggtt tgccactgcc atatatctgg
    15721 agtgccagag agtgtccgtc atcttgagat cagaggttac gccacagacg gcataaaaaa
    15781 tgttccaaac gggttattat ctctcagcat caatcgctac accccagaaa atcaggccag
    15841 aattgacagc ctgatatcac cgtcactaca gacgctatct ctgactggat gtagcaatat
    15901 tatactgccg gagaaactcc cggagagtct gagatctgta accattcatg tggagcagaa
    15961 aactacatgg aacatcgtag ctgaaggggt tcctgatgga ctggatcttg atttacaaaa
    16021 tgtactactc tctccgaatg taattaaaga aaaaaacatc acttttcagg gcaacgcact
    16081 ggatgctgcc ttgcattttc gtccgggaga cattgtctat gggctatcct ctcccagaga
    16141 aaaacttgtc aacagcatca aactggttaa cgacttttcc aaaaaagata tcgtaattca
    16201 gaatacatta acaaacgcag tatgggacgc cagaaccccc cgtaaatata agcaagactc
    16261 gcttatcaaa agagcattaa atgaacatga aagaggaaca aaatttaaac agtgcttaaa
    16321 aaatcacaat aattataatg tcatcatggc cgacctttcc atatacaatc gagacaagtt
    16381 atgggcaaaa acaagcaagg caggcctaga gtttcaaaca ttaattcgca acagaacggt
    16441 tatgttttgt gcggatgggc ttgtcaattc actcaaactc atagctaaca agtcagaggg
    16501 ctatggtcag agtattaccg ccagcgaatt acgatggatt tatcgtaata aagacaacag
    16561 ccaaataatg aaaaacataa aattttatct gcatggcaaa gagataccag cagaaagaat
    16621 attagataca ccagagtgga aagactaccg tccaaaatac tccggttcca catataaata
    16681 ttcttaatga tagcaaaaaa tatattttcg atataatcaa tgttatgatt aaagagtatt
    16741 tcatcagggc aggtaaaaac agagtaaatc agcaaaagaa gctgatcttc agcgatactg
    16801 acactaactg acggtttaag cggtcgtatg aagcagcagc tttccgacgg actgccatgc
    16861 ggatcgttta ccttttgggc tattccgccc gtcatcaagc ggctcacgag tactgagttt
    16921 atcaggatga tattgctgac aatggtaatt cgttgaccga tgtatacttc actatatatc
    16981 ggtcaacacc gtactgccgt gcaacatctc aaagatgtag catcgttttt accagaggag
    17041 gcaggacata gattgtaaaa aaactatttc gatgcattta ccattgagat attttccttc
    17101 ataccaaaag ttctatcaat tatcgcaagt ggttcaacag gtgcggtctt atctataacc
    17161 caaccactct ctgtaacact tcctcgtgtg atatattttg cctttacttt aacaactatt
    17221 agataatgct cacgactaaa ccgatcagca acgctaagat ccgatgtgta ttcaggcaat
    17281 attcttccaa tacgtacctg ctgttgtgcc atatattcat ctggtgcttt tacatttgga
    17341 tttgaatcag gaagccctcc agcagtttta tattcaacga tattttcagc ttgaataccg
    17401 gttgtgccgc gacacaatat gatgtctgaa tcggacagtt ctatgtgccc ctccttaatt
    17461 ttcgaataaa tttgtttaat actatgttct ggagttggta ctatttcaga agataaagca
    17521 ggcaacgtgt cttcaagcac cggagaatca tcgtatatat ccaacaaatc ctcatcatac
    17581 ccaatatctc cttctcttaa ctgagaatac ggtttatcta tgtatctaat aactccccca
    17641 ccatgaagac cttctgattt ataaaatgta ccatcagcgt cacgaaagaa ataaacatta
    17701 acctgtttgt tatttataga atacctacct gaatataatt ccgaatttgg ctccttagtt
    17761 aaatctactg attccccctt gtagactatt ttatttggtg tctctgaaga tatggggtta
    17821 aaggattcta tatatctata aaaaccaccg ccaactttct
//
LOCUS       DAZGJW010000091        17793 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZGJW010000091 DAZGJW010000000
VERSION     DAZGJW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.091"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            220..876
                     /gene="pphA"
                     /locus_tag="ACHKIK_003172"
     CDS             220..876
                     /gene="pphA"
                     /locus_tag="ACHKIK_003172"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO9084293.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(877..1068)
                     /gene="yebW"
                     /locus_tag="ACHKIK_003173"
     CDS             complement(877..1068)
                     /gene="yebW"
                     /locus_tag="ACHKIK_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO9084294.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1173..1409)
                     /gene="yebV"
                     /locus_tag="ACHKIK_003174"
     CDS             complement(1173..1409)
                     /gene="yebV"
                     /locus_tag="ACHKIK_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO9084295.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1527..2966)
                     /gene="rsmF"
                     /locus_tag="ACHKIK_003175"
     CDS             complement(1527..2966)
                     /gene="rsmF"
                     /locus_tag="ACHKIK_003175"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO9084296.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQATAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(3046..5679)
                     /gene="yebT"
                     /locus_tag="ACHKIK_003176"
     CDS             complement(3046..5679)
                     /gene="yebT"
                     /locus_tag="ACHKIK_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO9084297.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSVSGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5648..6931)
                     /gene="yebS"
                     /locus_tag="ACHKIK_003177"
     CDS             complement(5648..6931)
                     /gene="yebS"
                     /locus_tag="ACHKIK_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO9084298.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7061..7558
                     /gene="msrC"
                     /locus_tag="ACHKIK_003178"
     CDS             7061..7558
                     /gene="msrC"
                     /locus_tag="ACHKIK_003178"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO9084299.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7655..8353
                     /gene="proQ"
                     /locus_tag="ACHKIK_003179"
     CDS             7655..8353
                     /gene="proQ"
                     /locus_tag="ACHKIK_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO9084300.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8373..10421
                     /gene="prc"
                     /locus_tag="ACHKIK_003180"
     CDS             8373..10421
                     /gene="prc"
                     /locus_tag="ACHKIK_003180"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO9084301.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPDPVK
                     "
     gene            10613..11494
                     /gene="htpX"
                     /locus_tag="ACHKIK_003181"
     CDS             10613..11494
                     /gene="htpX"
                     /locus_tag="ACHKIK_003181"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO9084302.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(11540..12913)
                     /gene="yebQ"
                     /locus_tag="ACHKIK_003182"
     CDS             complement(11540..12913)
                     /gene="yebQ"
                     /locus_tag="ACHKIK_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9084303.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPARSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLKPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            13090..13881
                     /gene="kdgR"
                     /locus_tag="ACHKIK_003183"
     CDS             13090..13881
                     /gene="kdgR"
                     /locus_tag="ACHKIK_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO9084304.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(14024..14263)
                     /gene="yobH"
                     /locus_tag="ACHKIK_003184"
     CDS             complement(14024..14263)
                     /gene="yobH"
                     /locus_tag="ACHKIK_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO9084305.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            14422..14565
                     /gene="mgrB"
                     /locus_tag="ACHKIK_003185"
     CDS             14422..14565
                     /gene="mgrB"
                     /locus_tag="ACHKIK_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO9084306.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            14640..14927
                     /gene="yebO"
                     /locus_tag="ACHKIK_003186"
     CDS             14640..14927
                     /gene="yebO"
                     /locus_tag="ACHKIK_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO9084307.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            15596..15739
                     /gene="yobF"
                     /locus_tag="ACHKIK_003187"
     CDS             15596..15739
                     /gene="yobF"
                     /locus_tag="ACHKIK_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO9084308.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            15752..15961
                     /gene="cspE"
                     /locus_tag="ACHKIK_003188"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             15752..15961
                     /gene="cspE"
                     /locus_tag="ACHKIK_003188"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO9084309.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            16127..16936
                     /gene="rlmA"
                     /locus_tag="ACHKIK_003189"
     CDS             16127..16936
                     /gene="rlmA"
                     /locus_tag="ACHKIK_003189"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO9084310.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16933..17499)
                     /gene="mntP"
                     /locus_tag="ACHKIK_003190"
     CDS             complement(16933..17499)
                     /gene="mntP"
                     /locus_tag="ACHKIK_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO9084311.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4676 a         4288 c         4498 g         4331 t
ORIGIN      
        1 atgagcataa atttatttaa cacattaatt ctaaaagatt ttgttgtttg ttgacgaaaa
       61 caggaatcgt gttcggtctc tttttatctg ttaaaagcca gaagcatttc cttcgctgac
      121 tttatagtca accataacac acactctact gtctgagtcc agcgtttttt aacattcttg
      181 ttaagattat gtgatcttta gcgcgggagg aaaatattga tgaaacagcc tgcgcccgtt
      241 tatcagagaa ttgcgggtca tcaatggcga catatctggc tttctggcga tatacacggt
      301 tgtcttgagc agttgcgccg caaattatgg cattgtcgtt ttgatccgtg gcgagattta
      361 cttatctcag taggagacgt tatcgatcgt gggccgcaaa gtttacgttg tctgcagtta
      421 ctggaacaac attgggtttg tgcggtaaga ggcaatcatg aacagatggc gatggatgcg
      481 ctggcatccc agcagatgtc tttgtggttg atgaatggcg gcgactggtt tattgcgctg
      541 gcagataatc aacagaaaca agcgaaaacg gcgctggaaa aatgtcagca tttgcccttt
      601 attcttgaag tacacagtcg taccggcaaa catgttattg ctcatgccga ttatccagat
      661 gatgtttatg aatggcaaaa ggacgttgat ttgcatcagg tcttgtggag ccgctcgcga
      721 ttaggtgaac gccaaaaagg gcagggaatt acaggtgctg atcatttctg gtttggtcat
      781 acaccgttgc gacatcgcgt ggatattggc aacctgcatt atattgatac cggtgctgtc
      841 tttgggggcg aactgactct tgtgcaattg caataattaa aaatcaccat actcctgtgc
      901 tggtcgccag aaaccatcta taaaatcctc aatcggaaaa caaccgccat ggcggatccg
      961 ttgatcgctc atagaataaa gacactgctg ttccgtgttg tagacatcca caacaatatc
     1021 ttcacaaccg ccatccaggt agcaaacaaa aagtaccagc gcgaacattt catccccgaa
     1081 gtgtggtgcc gtaccgttaa gtttaggaga gattttacaa cgggggaata accaggacaa
     1141 ataacccgcc atgccgacgg gttctttttg gatcaggcaa gacgcataat ccaggcatca
     1201 gattgttgat cgtaacgggt acggtaaagg acagagtgtt cgccatttag cagggcaggg
     1261 atactggttt cagcatccca ggcatcgagt tgcatacata aatcggggtc agatttacaa
     1321 ggaatggtta acgttcgatc acccggcgat tcaccgtgta attcgccgtc gtcgatttca
     1381 aaagcgccta tgcgcacact ggttttcatc ttcgtgctct ccatttagct gccgattgtg
     1441 gtacgaccag tccgatcgca ccatttttca gcctagttga ggcagccgaa tgtgccagtc
     1501 aaaaagtgcg ctttttttgt gagctgtcag gcgttaccgg taaaaagttt cccatcgcgc
     1561 accagttcac gcggatagct gtttttcaat cgcgaaccaa tccgtttggc taaaccaatc
     1621 ggttgatgct ggaaagtaac caatacatca tccgccactg gcgcggcttg cgggtaaaca
     1681 tcgcgcccgc gataccactc ctccgcttcc tgcggtgtca gctcgaatgc attcatatta
     1741 tcgggtgagg caagagcaat aaccgcttca tgctgccagc gataaccttt gttgtgcgtc
     1801 tcggcaagtt taatccccaa ccgagaaaat cggactttac cgatcagggc ttcaatgccc
     1861 accgggaaca accacagttc tttgtcacgc tgccagaggc gcaggttttc atcccagttt
     1921 aagccaacac ctgcagtcgc ctgacgaatt tgtccagctt cgcgatcttt caccgggctg
     1981 aacggaaaat taccgacttt gtatttgggg gcgggtaagg cgggaatcgc ctgagtttta
     2041 cgcagacgag caacgaagaa gccttcgcag tcgtaaattt gtgggaaaac atgcaaaaag
     2101 ccttcttcgg tcagcgcttt gtttgcacca gggaagagat cgccaagtgg taaaaactct
     2161 actgcgtcgg ggtaagtctc tttcagccac aggcaaacgg cttcgttttc ttcctggttt
     2221 aaggtacagg tcgagtaaac cagcgtacca ccaggacgta atgcatgaaa ggcgctgtcg
     2281 ataagctccc gttgtgtagc tgcgatttcc tgattgcttt ctggtgacca gttttttagc
     2341 gcatcgggat ctttacgcac cacgccttcg ccagagcagg gagcgtccag caaaatggca
     2401 tcgaacattt ctggcactgc cgcaccaaac acgcggccat caaaatgtgt gagcgcaaca
     2461 ttactgatgc cacagcggct gatattggca tgtaacactt ttacccgact ggcggaaaac
     2521 tcattggcaa ggattgcccc ttcgttattc atccgcgcgg caatttgcgt cgttttggag
     2581 cctggcgcag ctgcgacatc catcacccgc tgtggtgcat tatcgtcagc aaacaaggcg
     2641 gcaacgggca acattgaact ggcttcctga atataaaaca ggccgcttaa atgctcggcg
     2701 gtactaccca atggcaatgc atcttcattg tcgcgttcaa tccagaaacc ttcttcacac
     2761 cacggaattg gcgtaagcgt ccagccataa ggagcggtta attgcaggaa atcagcaacg
     2821 gagattttca gcgtattaac gcgaatgctg cggcgcaacg ggcgctgaca ggcggcaaga
     2881 aaatcatcaa atgagagcgt cgaaggcatc gcttcgcgca tttgtgtcag aaaggcgtcc
     2941 gggaaataaa cggtgtgttg ggccacgagc atgtaccact ggcaaaaaaa caggcgcgca
     3001 gtttagcata aacgctccgg cgcaggcacg ccggagcagt gggcattatt tgggaagcgc
     3061 agttccccat tcacgccact ctttcggttc actttcctgc aacaggaagt gtttgccttc
     3121 ctgggctttc ggtgccagtg gcgtacccgg aggcgtggcg aaggcgatgc cgccacggat
     3181 aaactggtta aaggtgccgg ttttcactac gccgcccgtc agaccaaagt ccagactgta
     3241 acccgatgcc aaccagaaga cggaattgtt acgcaccagg tgttgatagc gtttactgat
     3301 gcgcatcgca atcatcacgc gatctgacaa tgtccccagc gtcattcctg taaccgtacc
     3361 gacttccaga ccacggaaca gcacaggcgt accaatacct aacgaaccgg cttccggcgc
     3421 ttcaacaata atgcttaagc catccaggta acgcgaatca gtaatggtgg cctcttgtaa
     3481 ttcaaagtcg cggcgaggat tgccccggcc tggttcgacg ttgatatacg gctggaggat
     3541 agtatctaga tgctcaacgc ccgcggctga aatttgcggt gtgaccaccg agaagcgcgt
     3601 accgccgcgg gcgaaggtct ggacatactc cggatagagc accgcctttg cctgtacttc
     3661 attgcgcgcg gtaatcagat ccagcgtctg gatttgcccg atatcaatac cgagatagcg
     3721 aattggcatc ccgaccgcca gttttccggc atcgaaagcg tgaagcgtaa tctgcccacc
     3781 aaccgcacgg gccgctgttt cggaagcata cagaattcgt ttgtcgcctt tacgctgact
     3841 ggcgctggca ccgctgaggt tatcgaagct aatggctccc tttaatgctc tggagagcgg
     3901 ggatgcctgt acggtcaggc cactaccatt cagctgaact ttcgccccgc cttctgccca
     3961 gaacacgcta ttgctggtca gaaggttgcg atactccggc ttaatatgca gatcgatatc
     4021 aaacgcgtta gctcgcggac ggacggtaat aacttcacca acttcaaatt tacggtagag
     4081 cactaccgat cctgcctgca catccggcag cgtctctgca ctcaaactca cggttgtggt
     4141 gggtaaatcg ctaaggctgt tctccagcgc tttttccaga ttggcataca gtggatagct
     4201 ggctttcatc tcgcctttat cgcccggcag aatacgtatc ccgccgttaa tccattctga
     4261 ggcgctggca ccgagaaact caacgccatc cagccccacc ttcacgtcga cacggctgtt
     4321 gacgacaaat ttgctatcgc cttttaccag ttctcgatgc tgaggctcga tggcgacggt
     4381 aaaggtgacg cctttgctgg tgagtttacg atcgataacc tggcctacct gcacgccgtg
     4441 aagaatgagc ggctgaccag catcaatacc gtaactttcc ggcgcggtca gggtcagcgt
     4501 cagaacatca ggttcatgca gcagtgcttt ttcgcctggc acaacaacga actctttgcg
     4561 tggctcgcca tcgccgggta ccaactcgaa ggttttgcca gtcagcaggg cgctgagatt
     4621 ggcatcgcta agggataatt tcggattacg taattcgatg cgggtatttt cacgaagcag
     4681 ggtaacgacg ctgggatcaa ctgtcatttc cccagtgact ttaccaccag gatttaaatc
     4741 cagtttagtt agctgtccga cttccagccc ctgatacatt aacggcgtcg agtcggcggt
     4801 caatccggcc ccactcggca gttccagttt tattattacg ccacgctggc tgtgggccag
     4861 atcttcatac agaccaaagg tatcttccgc ctcggcaggt ttcgactctt caggtgaatc
     4921 gaaggcaatc gcaccgttaa ccagtgccgc cagactttcc agtttcacct tcgcgccact
     4981 gacactgacg ttggcatcaa cgccggaaac gttccagaaa cggctacctt ttttcaccag
     5041 atcggtaaaa cgccgctcga tcagaacatc aatcaccacg ccttgcttgt tgggattgat
     5101 ggcatagtcg tagacttttc ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt
     5161 cagcgaaccg agatcgggag cttgcaggtg gatcatcaga tcgccgttat ccagccgata
     5221 cttcggttgg gtatcgagtg cgacaaagtg atcctgctct ttacctttac ccggcatcat
     5281 gccgatatag ttcccaccga cgagggcgtc cagcccggag acacctgcca acgatgcttt
     5341 tggcgtcacc agccagaatt gagtctcttc gcgcagcgca tctttcatat cggacttgat
     5401 gctgaccttg acttcaatct tacgaagatc gtcgctgagg ctgatatcct gcactgttcc
     5461 gacttcaacg ccctgataac gaacaggcgt acggcccgga acaataccat ccgccgacat
     5521 aaagtcgatg gtgacggtat taccccggtc ctgataactg tcccaaatca gccaaccggc
     5581 aatcattagc gcgatgaaag gcagcagcca gaaaggtgag atacggcgtt tatttttaat
     5641 ctgcgcttca gtcgtcgaag cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc
     5701 tgtccagcca ttccacagca agaatagtca aaattacggc tgcgccaaaa taaaacgcag
     5761 ccggtcccat agtaaaagcg aggatctgat cgcgattaat cagcgacatg gttaaagata
     5821 tgacaaacag gtcgagcatc gaccagcgtc caatccaggt caccatccgc agtaacagaa
     5881 tgcgtgtgcg taaaccttgc tggcatttaa aatgaatgct caacagtaaa gtgaacatga
     5941 cgatcacttt agtaaacggt accagaatac tggcgataaa cacgattccc gcaaccgcaa
     6001 tgttgctact tgccagcgac ataattccgg aaagaattgt atcttcctgc cgtcctccgt
     6061 tcagataaat gatagaaata ggcaataggt tggctggtaa caacaaaacg attgacgcta
     6121 acagcgccgc ccagcatttt tgcagactat gacggcgacg caggcgtagc gggatatggc
     6181 aacgcgggca gcgaccacgc tgatcaggat agccggtaaa atggcaccca agacagacac
     6241 gaagtttctc gtccctacgc gtagcggggc gctgcggata atatcgctcc cacagctcct
     6301 caacattaag atgtgacaac gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca
     6361 agccgacacc agcctggata tgggcataat cctgtacctt tatagaagca acgccaatgc
     6421 cgaccaggta gatatccagc attacccact ctttaagacg ctcaagcatc agcagtaccg
     6481 gacgtaaatt cattcccagt cggttaccaa accataaata tgctatggag gacaccagaa
     6541 tgaggggggc accaataacg cagaaaaaga ccatcgcccc cgttatcgta tcgccctgtt
     6601 tggtcatttg ccagatgcct tgcatgacgt tggcgtcgat acgaatacct aacagccaga
     6661 tatgcaacag cggttcgccc caggcaaacg gcatcagcaa cagcatggtg aaagccattg
     6721 ccgccaggcg cgttagcgac cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac
     6781 aataggcact ctgatgagaa tttatctcgg gcaggctaaa cagcatgtca cattgcgggc
     6841 aacgttggta atcaccacgc ggcagttcct cgccgattgc cctcactgtt atcttttttg
     6901 tcggcgtaat ttgtggtgtg ttaagagcca tgacgtgcca aatgtaataa gaattaacat
     6961 taatattaac ccatgattgg aatgatcttg agcattaacg catttaatgc ttattttaat
     7021 aggctggcag ttccaggtaa gacaaccaag tgattatata atgaacaaaa cagaatttta
     7081 cgcggattta aatcgcgact ttaacgcgct gatggcggga gaaaccagtt ttctggcaac
     7141 gcttgcgaac accagtgcgt tgttatacga gcgtctcact gacgtaaact gggcaggttt
     7201 ttatttgctt gaggacgata cactggtact aggaccattt cagggcaaaa ttgcctgtgt
     7261 ccggataccc gtcgggcgcg gggtgtgcgg cactgcggtt gcccgcaatc aagtgcagcg
     7321 tatcgaggat gttcacgcgt ttgacgggca tattgcctgt gatgcggcga gtaattctga
     7381 aattgttctg ccgctggtgg tgaaaaatca gattattggt gttcttgaca tcgatagtac
     7441 cgtcttcggt cgctttacag acgaggacga gcaaggctta cgtcagcttg tggcacagct
     7501 tgaaaaagtg cttgcaacga cggattacaa aaaattcttt gcgagcgtcg caggataatc
     7561 aacggataac gtagcattta ctggtagcgt cattataatg acgcctgttc atgcctgcgc
     7621 ttgttggcta cgtccgttgt aatcaggaaa tttcatggaa aatcaaccta agttgaatag
     7681 cagtaaagaa gtaatcgcgt ttctggccga acgttttccc cactgtttca gtgcggaagg
     7741 tgaagcgcgt ccgctgaaaa tcggtatttt tcaggatttg gtcgatcgtg ttgccgggga
     7801 aatgaacctg agcaaaacgc aattacgatc cgctttacgt ctctacactt cgagctggcg
     7861 ttatctttac ggtgttaaac ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg
     7921 tgagctggac gagcaacatg tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg
     7981 tgttcaggca cagcgtgctg aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg
     8041 tgagaaagaa gacgcaccgc gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa
     8101 agaaggcgct gaacgtaaac ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt
     8161 aaaagcacct cgcgaagaac agcacacccc agtttctgac atttcagctc tgactgtcgg
     8221 acaagccctg aaggtgaaag cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac
     8281 caaagacggc gtccgcgtcc agctgaattc gggtatgtct ttgattgtgc gcgcagaaca
     8341 cctggtgttc tgaaacggag gccgggccag gcatgaacat gttttttagg cttaccgcgt
     8401 tagctggcct gcttgcaata gcaggccaga ccttcgctgt agaagatatc acgcgtgctg
     8461 atcaaattcc ggtattaaag gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt
     8521 cgcgcttcac ccgttctcat tatcgccagt tcgacctcga tcaggcattt tcggccaaaa
     8581 tctttgaccg ctacctgaat ctgctcgatt acagccacaa cgtgctgctg gcaagcgatg
     8641 ttgaacagtt cgcgaaaaag aaaaccgagt taggcgatga actgcgttca ggcaaactcg
     8701 acgttttcta cgatctctac aatctggcgc aaaagcgccg ttttgagcgt taccagtacg
     8761 ctttgtcggt actggaaaag ccgatggatt tcaccggcaa cgacacttat aaccttgacc
     8821 gcagcaaagc gccctggccg aaaaacgagg ctgagttgaa cgcgctgtgg gacagtaaag
     8881 tcaaattcga cgagttaagc ctgaagctgg caggaaaaac ggataaagaa attcgtgaaa
     8941 ccctgacacg ccgctacaaa tttgccattc gtcgtctggc gcaaaccaac agcgaagatg
     9001 ttttctcgct ggcaatgacg gcgtttgcgc gtgaaatcga cccgcatacc aactatcttt
     9061 ccccgcgtaa taccgaacag ttcaacactg aaatgagttt gtcgctggaa ggtattggcg
     9121 cagtgctgca aatggatgat gactacaccg ttatcaattc gatggtggca ggtggtccgg
     9181 cagcgaagag taaagctatc agcgttggtg acaaaattgt cggtgttggt caaacaggca
     9241 agccgatggt tgacgtgatt ggctggcgtc ttgatgatgt ggttgcctta attaaagggc
     9301 cgaagggcag taaagttcgt ctggaaattt tacctgctgg taaagggacc aagacccgca
     9361 ctgtaacatt gacccgtgaa cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga
     9421 agaccgtcgg taaagagaaa gtcggcgtgc tggatattcc gggcttctat gtgggtttga
     9481 cagacgatgt caaagttcaa ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca
     9541 tcgacctgcg tagcaatggc ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt
     9601 ttattcctgc gggtcccatt gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca
     9661 gcgataccga cgggcaggtg ttctataaag gcccgctggt ggtactggtt gaccgtttca
     9721 gtgcttcggc ttcagaaatc tttgccgcgg caatgcagga ttacggtcgt gcgctggttg
     9781 tgggtgaacc gacgtttggt aaaggcaccg ttcagcaata ccgttcattg aaccgtattt
     9841 acgatcagat gttacgtcct gaatggccag cgctgggttc tgtgcagtac acgatccaga
     9901 aattctatcg cgttaacggc ggcagtacgc aacgtaaagg cgtaacgcca gacatcatca
     9961 tgccgacggg taatgaagaa acggaaacgg gtgagaaatt cgaagataac gcgctgccgt
    10021 gggatagcat tgatgccgcg acttatgtga aatcaggcga tttaacggcc tttgggccgg
    10081 agctgctgaa ggaacataat gcgcgtatcg cgaaagatcc tgagttccag aacattatga
    10141 aggatatcgc gcgcttcaac gctatgaagg acaagcgcaa tatcgtttct ctgaactacg
    10201 ctgtgcgtga gaaagagaat aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac
    10261 gctttaaacg cgaaggtaaa ccggagttga agaagctgga tgatctaccg aaagattacc
    10321 aggagccgga tccttatctg gatgagacgg tgaatatcgc actcgatctg gcgaagcttg
    10381 aaaaagccag acccgcggaa caacccgatc ccgtcaagta atatcaatca ggcacaagaa
    10441 attgtgcctg attttttaac agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa
    10501 gttgtgtctt tctggtgact tacgcactat ccagacttga aaatagtcgc gtaacccata
    10561 cgatgtgggt atcgcatatt gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg
    10621 aatcgcgctc ttcctgctaa cgaacctggc cgtaatggtc gttttcgggc tggtactgag
    10681 cctgacaggg atacagtcga gcagcgttca ggggctgatg atcatggcct tgctgttcgg
    10741 ttttggtggt tccttcgttt cgcttctgat gtccaaatgg atggcattac gatctgttgg
    10801 cggggaagtg atcgagcaac cgcgtaacga aagggaacgt tggctggtca atactgtagc
    10861 aacccaggct cgtcaggcag ggatcgctat gccgcaagtg gctatctacc atgcgccgga
    10921 catcaacgct tttgcaaccg gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg
    10981 tttgctgcag aacatgagcc cggatgaagc cgaggcggta attgctcacg aaatcagtca
    11041 catcgccaat ggtgatatgg ttaccatgac gctgattcag ggcgtggtga acaccttcgt
    11101 tatctttatt tcccgtattc tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga
    11161 tgaaggtgaa gagagcaacg gcaacccgct gatctacttt gcggttgcaa cggttctgga
    11221 actggtgttt ggtattctgg cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt
    11281 ccatgctgat gccggttcgg caaaactggt tggtcgcgag aaaatgattg ccgcgctgca
    11341 gcgcctgaaa accagctatg aaccgcaaga agcaaccagc atgatggctt tctgcattaa
    11401 cggtaagtcg aaatcgctca gtgagttgtt catgactcac ccgccgctgg ataaacgaat
    11461 tgaagctctg cgtacgggtg aatacctgaa gtaatatgaa gtaaatacta aaaggcgcgt
    11521 cctgatcgac gcgctttttt tatgccctgg atcgtggctg agtgatacgt aaaccactca
    11581 cacaggcagc aatcactgcc agaatagccg cagccatcag cgaaacgtgc gtaccattat
    11641 caccgaactg atttagcatc agcgccacca gcgccgcgcc actactctga cccagtagac
    11701 gagccgttcc taacatgcca ctggccccac cgctacgttc gcgcggagcg gaggtaataa
    11761 tggtgtggtt attgggtgac tggaataagc caaatccagc gccacataag atcatcggcc
    11821 agataatatt gatatccgca ggtgacgcgg gcagcagaac cagggaaaaa agccccgcag
    11881 ccatgatgaa caaccctaaa gcacccagca atcctgcatg tacgcgttca atcaaatagc
    11941 ctgccagcgg agccatcacc atcgttgcta acggccacgg tttcagaagt aaacctgttt
    12001 cgacttcact acgcccgagc acggtttgca ggtaaaaggg cagggaaacc attgccagca
    12061 tttgtgcgca gaaagagcaa acagatgtgc aaatagaaag tgaaaacagc gggatacgca
    12121 gtaaatccac cggtagcagc ggtacgggaa gagaaagctg gcggcgaata aagaaaatac
    12181 caacaacaac cattaccacc agttccgcag caattaacgt cagcgattgc ccctgagcga
    12241 aaccactcaa cgcagtgata agcaggccga aggttaacgc gttcatcacg gcgctgggca
    12301 ggtcgaaacg gggtttactg gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa
    12361 gcagggcgat aatacctaac ggtacgttga ttaaaaataa ccatttccag gatgcgatgg
    12421 agaggattgc tgcagcaatt gtcggcccgg cagcagaaga aacggcaaca ataaacgagt
    12481 ttatgcccat ccctctcccc agaaaacgtt gtggatagat caggcggata agtgcggtat
    12541 taacgctcat caacgctgca ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt
    12601 gcagcgaatc agaaagggcg cagaacagtg aagacaacag aaaaacgacc agaccgcatt
    12661 tataaatacg tcgatagcca aacatatcgc ccagaaacga aaacgagagc agggagatga
    12721 caatggcgat ttgataggcg ttcactaccc agatggatct ggctggcgtg gcatgaaggt
    12781 ccgtggcgat tgttggcagg gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa
    12841 tgccaatcac aatggttaat atcgcaccgt atcgctgggg caatggcagg ccgtcggcct
    12901 gaacttttgg cataggaatt ttatatcttt ggtgaataat caacaggggt atgattctag
    12961 cactgatatt cacggtggat gtcgcagaat tgtaactaac cggcaaaaga tggattgcga
    13021 ccaccctggt gcgaaattat aataaaaaac agttctgatt tttataaaac actcgcaatg
    13081 aggtgataaa tggctaacgc agatctggat aaacagcctg attctgtatc ttccgtgcta
    13141 aaagtttttg gcattttgca ggcgctgggt gaagagcgcg aaatagggat aaccgagctg
    13201 tcgcagcgcg tcatgatgtc aaaaagcacc gtttatcgct ttttacagac catgaaaacc
    13261 ttaggttatg tggcgcagga aggggagtcg gagaaatatt ccctgaccct gaaattgttt
    13321 gaactgggcg ctcgcgcgtt acaaaacgtc gatttaattc gtagcgcaga tatccagatg
    13381 cgtgagatct cccgcctgac caaagaaact atccacctcg gcgcactgga cgaagacagt
    13441 attgtttaca ttcacaaaat tgactctatg tacaatttgc gcatgtattc acggattggg
    13501 cgtcgtaatc cgctgtacag caccgcgatt ggtaaggtac tgctggcatg gcgcgatctc
    13561 gatgaagtga agcaaattct tgagggcgtg gagtataaac gcagtaccga gcggaccatc
    13621 accagtacag aagcgttatt acccgttctg gaccaggtgc gcgagcaggg gtatggcgaa
    13681 gataacgaag agcaggaaga agggctgcga tgcattgcgg taccggtatt tgatcgcttt
    13741 ggcgtggtca ttgccggttt gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt
    13801 ttacaggaat atgtcgcaat gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt
    13861 tatcacgact atccgttctg atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt
    13921 ctttatttag catgagaaaa ataaagttga aggtgcgtta tattaaacgc gcttgctata
    13981 agaatatttt actcatgagt gagaatctgg ttatttatta cccttaacca ttatcgacca
    14041 cgatattgct tttacgtaac agtgggcaat ctgttatccc caagaaacca cttttagtgt
    14101 gcaagtattg taccgtgctg gtgcctctgg cagtcagata ggtacattgc aaacctaatc
    14161 ctgcggcatt ctctttgctt ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc
    14221 ataccagtgc tatcagcata actgtgcgaa tgatgaatcg cattacaacc tcttctcttt
    14281 ttatgttcgc ttaatcgtag cggcaatatg cgctgaagca agctactcat tccgaaaaag
    14341 cacgaatatc gacatagtta ggcgctgttt aactaacgca tgctagttta atgacataag
    14401 gtaggtgaag cggagattgg agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg
    14461 ttggcttgct tgctgctttg ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa
    14521 tttttcagcg gaatttgtgc cattaaccag tttatcccgt ggtgatatca ttttttcagc
    14581 actagatttt cttcctctcc agcagtggta gaatagcgcc tctactcaga ggtggtgaaa
    14641 tgaacgaagt tgtaaattca ggcgtgatga acattgcgtc tttggttgta tcggtggtgg
    14701 ttcttcttat cgggctcatc ttgtggtttt ttatcaatcg tgccagttct cggactaacg
    14761 aacagattga actgcttgag gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac
    14821 gtcgtttgtg cgaagcaaac gaacctgaga aagcagataa aaagaccgtt gagagtcaaa
    14881 aatcggttga agacgaagat attattcgtc tggtcgccga acgataatcc ttcctcaatc
    14941 ttgagtaacc ccgtcgttga agggttactc aattcatcct gattatacgc actgccatgg
    15001 tgcaactgct tcaaattaaa actctggtct gacttcaaat aattatcata ttatttattt
    15061 gcgtaacggg atatcagaaa aagatatttt cctcattaat ttattaggat gtttacatcg
    15121 gatttgtgat taagcgtggt attatttatt agcgaaacgt ttctctcttg attttttgct
    15181 cattcatcaa tttttcttat tttaaatttt caatcccttt ggggattgac ttctctttag
    15241 ggtaattgat agccgttaac tgactgtttt atgagaaaaa gtgatataac tttttattca
    15301 ttgcatagca aaaaatgtga tattgcacgc actatgtaat aacttctccc actggcctgg
    15361 aacaactgaa cttattgaac tatgttagaa aatacgccag tttaagtatc tgcctgaact
    15421 ggcaaggtta agcacaatga tatatcggcg cgtattccgt tgcataagtg tgcaaaaaaa
    15481 gtggaagacg tatcgagatt tgtgcgtctg atcgagacat gtttaaaaat ggcttgccat
    15541 aattaacgtt gtatgtgata acagatttcg ggttaaacga ggtacagttc tgtttatgtg
    15601 tggcattttc agtaaagaag tcctgagtaa acacgttgac gttgaatacc gcttctctgc
    15661 cgagccttat attggtgcct catgcagtaa tgtgtcagtt ttatctatgt tatgcctgcg
    15721 ggcgaagaaa acaatctaag gaatttttca aatggcaaag attaaaggtc aggttaagtg
    15781 gttcaacgag tctaaaggtt ttggcttcat tactccggct gatggcagca aagatgtttt
    15841 cgtacacttc tccgctatcc agggtaatgg cttcaaaact ctggctgaag gtcagaacgt
    15901 tgagttcgaa attcaggacg gccagaaagg tccggcagct gttaacgtaa cagctatctg
    15961 atcgaatcca ctgatctgaa gtgtgaatac gcttcaatct cgctataaag cctcgtcgaa
    16021 tgcgaggctt tttactatgc tttatcttcg ctcctggcgt tcggatattt gcccgccgcg
    16081 tgattcgcgt tacacttgcg gcctttagta tcctgccgga gttgtcatgt cgttttcctg
    16141 tccactttgc catcagcctc tttcgcgtga aaaaaacagc tatatctgtc cccagcgaca
    16201 tcagtttgat atggcgaaag aagggtatgt caatctgctg cccgttcagc ataaacggtc
    16261 tcgtgatccg ggcgacagcg cggaaatgat acaagcacgc cgcgcattct tagatgccgg
    16321 acattatcag ccgctgcgtg atgcaattgt cgcccaactg cgggaacggc ttgatgaaaa
    16381 ggccacggcg gtgctggata ttggctgtgg tgaagggtat tacacacacg catttgccga
    16441 tgtgttgccc gaaatcacca cgtttggcct ggatgtctcg aaggtagcga taaaagcggc
    16501 ggcgaaacgc tatccgcagg tcactctttg tgtcgcttcc agccaccgtt tgccgttttc
    16561 cgataccagt atggacgcca taatacgtat ttacgcgccg tgtaaagcag aagaattagc
    16621 acgagtagtg aagcccggcg gctgggtcat tactgccacg ccgggaccgc gacatttgat
    16681 ggagctgaag gggctgattt acaatgaagt acatcttcat gcacctcatg cagaacaact
    16741 ggaaggtttt acattacagc agagtgatga gttgtgttat ccgatgcgtc ttcgcggtga
    16801 tgaagccgtc gcattattgc agatgacgcc gtttgcctgg cgtgcgaagc cagaagtctg
    16861 gcaaacactg gcagcaaaag aagtgttcga ctgccagacg gactttaata ttcacctctg
    16921 gcagcgttct tattaaccgt ggaagtgcgt ccagaggatc tggacgccga tgccgatcag
    16981 caccagcccg ccgagaattt ccgctttttt cccaataatt gagccgataa agcgaccaac
    17041 catcatccct aatgtcgaca taatcaaggt tgcacaacca atggccaatg cggtcgcgat
    17101 aatgttgacc tgcaggaaag caagaccaac acccacagcc atggcatcca ggctggtggc
    17161 aatcgcggtg gttaccagta gccagaaacc gtgccgacgg cgcggctctt catcttcatc
    17221 atctgcgcca cgaaaaccct caataatcat tcgcccgccg aggaatatca gcagcacaaa
    17281 cgcaatccag tggttccatt caaggacaaa ccggctggct aacatgccca ttccccagcc
    17341 gatcagcggc gtcagggttt cgacggcacc aaaaataagg ccggttcgca atgcttcaga
    17401 aaatttcggt ttatggaggg tggcaccttt accgattgat gcagcaaatg catccatcga
    17461 cataccaaac gcaagaagaa cagtagcagt gatattcatg acaatgtcct gaccggggta
    17521 tccataaaac acatcatcgc ccccagcaaa catgcataca tgaacaacaa gccgcgatgg
    17581 ttttcgcgtt catgcaggca tagcgattga tgtgcatatg gtctcgcctg actgaaagta
    17641 ttcagtccgt acgtgccacg ttttgcaacg agtatgttga cacgtacatt tccggaatct
    17701 ggaaatcggc tactccccaa atgagggcgc aaccttaaca tattttgaga atataaaaca
    17761 actgttggaa tatattattt cattgcttta ttg
//
LOCUS       DAZGJW010000092        17640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZGJW010000092 DAZGJW010000000
VERSION     DAZGJW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.092"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            205..411
                     /locus_tag="ACHKIK_003191"
     CDS             205..411
                     /locus_tag="ACHKIK_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084312.1"
                     /translation="MNKEQSADEVSLDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
     gene            984..1511
                     /locus_tag="ACHKIK_003192"
     CDS             984..1511
                     /locus_tag="ACHKIK_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO9084313.1"
                     /translation="MSNVSGIGDAYYWSVFKIAEAFGLHRDTVKKRLLAANTPVAATV
                     RGNPVYALQHVGPALFSVKHEAADSVHDPSRMEPKERKDWYQSENERIKLEKEQRKLI
                     PVDEVVIVYSSMRKAVVQVLETIPDVLERDCALTPQAVGVVQQAIDDLRYTLQEKSYE
                     ACAAELIPDEEGESL"
     gene            1520..3619
                     /locus_tag="ACHKIK_003193"
     CDS             1520..3619
                     /locus_tag="ACHKIK_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO9084314.1"
                     /translation="MGFSSARNLGRDISAGFSPPRRMPISEAVKKFMRVPKGAGNSVP
                     WDPELTPYIIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWIVYTIVCDPSDMLVVQ
                     MTEDKAREHSKKRLDRTFRSSAAVKKRMSPRRNDNNVHDKTFRDGSFLKIGWPSVNIM
                     SSSDYRFVALTDYDRFPENIDSEGDGFSLASKRTTTFMSAGMTLVESSPGRDICDSKW
                     RRKSPHEAPPTTGILSLYNRGDRRRWYWSCPHCGEYFQPAMDAMTGYRNEPDPFKASE
                     AAYLLCPHCSGIITAEKKRELNSAGVWLREGQVIDRNGNVSGEPRRSRIASFWMEGPA
                     AAYQTWAQLVYKLLTAEQEYEATGSEETLRAVINTDWGLPYLPRASMEQRKSELLEQR
                     AEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRSD
                     CDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDNA
                     YKFWRRCRRDGLGKRIYLFKGDSIRRAKLISRTFPDNTGRTGRRAQAAGDVPLWLLQT
                     DALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAFD
                     LMVYAEALVILHGYEKIRWPDAPEWASRETWLECVQDSTEPSSSPEPVSTPVKKQKRK
                     KTVTDDVNPWLTSGGWL"
     gene            3616..3828
                     /locus_tag="ACHKIK_003194"
     CDS             3616..3828
                     /locus_tag="ACHKIK_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084315.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            3828..5336
                     /locus_tag="ACHKIK_003195"
     CDS             3828..5336
                     /locus_tag="ACHKIK_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9084316.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLAP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYEPDGNGRKENERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            5281..7308
                     /locus_tag="ACHKIK_003196"
     CDS             5281..7308
                     /locus_tag="ACHKIK_003196"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO9084317.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQSLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            7395..7718
                     /locus_tag="ACHKIK_003197"
     CDS             7395..7718
                     /locus_tag="ACHKIK_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFO9084318.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGITEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSEPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            7711..7986
                     /locus_tag="ACHKIK_003198"
     CDS             7711..7986
                     /locus_tag="ACHKIK_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO9084319.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMIVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            7998..8576
                     /locus_tag="ACHKIK_003199"
     CDS             7998..8576
                     /locus_tag="ACHKIK_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9084320.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTVRIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGSLTQ
                     AFEDARDHIIAAEMPKQLGYALKQQLRLWLTR"
     gene            8573..8974
                     /locus_tag="ACHKIK_003200"
     CDS             8573..8974
                     /locus_tag="ACHKIK_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO9084321.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8985..9728
                     /locus_tag="ACHKIK_003201"
     CDS             8985..9728
                     /locus_tag="ACHKIK_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9084322.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVSPASASVKVGATTTLTFTVKPDGASDKAISVHSSDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVTAVTVSAAG"
     gene            9789..10175
                     /locus_tag="ACHKIK_003202"
     CDS             9789..10175
                     /locus_tag="ACHKIK_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO9084323.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAGEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAVDDEHEALTPEKS"
     gene            10184..10513
                     /locus_tag="ACHKIK_003203"
     CDS             10184..10513
                     /locus_tag="ACHKIK_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO9084324.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            10485..13550
                     /locus_tag="ACHKIK_003204"
     CDS             10485..13550
                     /locus_tag="ACHKIK_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9084325.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRLNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARVAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            13550..13879
                     /locus_tag="ACHKIK_003205"
     CDS             13550..13879
                     /locus_tag="ACHKIK_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9084326.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCDHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            13889..14587
                     /locus_tag="ACHKIK_003206"
     CDS             13889..14587
                     /locus_tag="ACHKIK_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9084327.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVIWQGRQYQVYPIQGTGFELNGKGSAARPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14592..15335
                     /locus_tag="ACHKIK_003207"
     CDS             14592..15335
                     /locus_tag="ACHKIK_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO9084328.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNLEAT
                     GLYQVALSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFE"
     gene            15233..15880
                     /locus_tag="ACHKIK_003208"
     CDS             15233..15880
                     /locus_tag="ACHKIK_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO9084329.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGSEAIRAL
                     AMQIPAFRQKLSDGWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15941..>17640
                     /locus_tag="ACHKIK_003209"
     CDS             15941..>17640
                     /locus_tag="ACHKIK_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO9084330.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQNRPSDKAWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPEGVAEYSVWGLKLPTL
                     R"
BASE COUNT         4226 a         4126 c         5360 g         3928 t
ORIGIN      
        1 atgtcaaata ttaagcctgt ttttaatgaa tagtgttgat tgcgtaacaa aattgagcct
       61 tgctggcatc caggagggat atgcaaccga cagatgtatg taaggtcgat gtactcaaac
      121 tttcatactt ttcctctttt atgcagtaag atttgaagta atattttaac cgctagatga
      181 cgagcaaacg catggagcga caaaatgaat aaagaacaat ctgctgatga agtctcgttg
      241 gatctgattc gtgtaaaaaa tatgcttaat agcaccattt ctatgagtta cccggatgtt
      301 gtaattgcat gtatagaaca tcaggtgtct ctggaagcat tcagggcaat tgaggcagcg
      361 ttggtgaagc acgataagaa ttcgaaggat tattccctgg tggttgactg agcaccataa
      421 ctgctaatca ttcaaactat ttcacctgtg acagagtcaa tatcgcattc tgtcactgtc
      481 aggctaatac agagctgcaa ttcaactact gcaatgtcct cgtaattagg tgaatttaca
      541 atatcgtcct gttcggatgc cggttgcatt gctgaagatg aggcatttat ggttcgcata
      601 ttttcccctc atgctcgtca gtcctgtgcg taggaagaaa caggacactc acactaattt
      661 gtgtgggcat gctgtgatgt ccttctgaat tattcctatg ccattatgta aagcgctgta
      721 tcagatgctc atcacggctg tcaggctgtc aggctgtcgg gtcctcccgg tgggggcccc
      781 tgccacgggg cgggagcgtc gcggaaaaag gctagttttt gcatttccat ggcggcggca
      841 gcatgtttgg taatttattg ataattaaaa gttatttctc ttttcacctg tacaatattt
      901 ctttctccct gtcattagac cagtttgcaa ttaattgaaa tatataaata aacatgattt
      961 tcacctgcca gatggagttg cttatgtcaa atgtgagcgg gatcggtgat gcttattact
     1021 ggagtgtttt taaaatcgct gaggcctttg ggcttcaccg ggacacagta aaaaaacggc
     1081 tcctcgcggc taacactcct gtggctgcga ctgtcagggg gaaccccgtt tacgccctgc
     1141 agcatgtcgg gccagccctg tttagtgtga agcatgaggc agcagactct gttcatgatc
     1201 catcccgtat ggagccgaaa gagagaaagg actggtacca atctgaaaat gaaaggatca
     1261 agctggaaaa ggagcagcga aaactcatcc ccgttgatga agtagtcatc gtctattcgt
     1321 ccatgagaaa ggctgtcgtc caggttctgg agacaattcc ggatgttctt gaacgcgatt
     1381 gcgccctgac tcctcaggcc gtcggcgttg tacagcaggc cattgatgac ctgcgataca
     1441 ctcttcagga aaaatcctac gaggcttgtg ctgctgaatt aattcctgat gaggaaggag
     1501 agagtctcta ggaggaataa tgggtttttc atcagcccga aatttgggaa gggacatatc
     1561 ggcaggattt tccccaccac gtcgcatgcc gatttcggag gctgttaaaa aattcatgcg
     1621 tgttcccaag ggggctggta actcggtgcc atgggatcct gaactgacac cctacatcat
     1681 tgagcccatg aactgcctgg catcgcgtga atacgatgcg gtgatttttg ttggtcctgc
     1741 gcgaacaggg aagaccattg gtctgatcga tggatggatt gtctatacca tcgtttgcga
     1801 tccttcggac atgctcgttg tgcagatgac cgaagataag gcccgcgagc attctaaaaa
     1861 gcgcctcgac agaacgttca gaagcagtgc ggcggtaaag aaaagaatga gtccacgtcg
     1921 taacgacaat aatgtccatg ataagacgtt cagggatggc tcgttcctta aaattggttg
     1981 gccctcggtc aacattatgt cgtcgtcgga ttaccggttt gtcgccttaa ccgattacga
     2041 ccgttttccg gagaatatcg acagcgaggg tgatggtttc tccctggcct caaaacgtac
     2101 caccacattt atgtccgctg ggatgactct ggtggagagc tcgccgggac gtgacatctg
     2161 cgacagcaaa tggcgacgta agtcgcctca tgaagcgcca ccgacgactg gtattctttc
     2221 cctttacaat cgtggtgacc gccgccgctg gtactggtca tgtccgcact gcggtgaata
     2281 ttttcagcca gccatggatg ccatgaccgg ctaccgtaat gaaccggatc cctttaaagc
     2341 cagtgaggcg gcgtatctac tttgcccgca ctgcagcggc attatcactg cggagaaaaa
     2401 gcgtgagctc aatagtgcag gagtctggtt gcgtgaaggt caggtcattg atcgtaacgg
     2461 caacgtttcc ggtgaaccgc gccgctcccg tatcgccagt ttctggatgg aagggccagc
     2521 tgctgcgtat cagacctggg cgcaactggt ttacaaatta ctgactgcag aacaggagta
     2581 tgaagcgaca ggaagcgaag aaacactcag ggcggttatc aacaccgact ggggattgcc
     2641 ttatcttcct cgtgccagca tggagcaacg aaaaagtgaa ctgcttgagc agcgggcaga
     2701 gccagttcct tcccgcagtg tgccggatgg cgttaatttc cttgtggcga cagtggatgt
     2761 gcaggcggga cgtcatcgcc gttttgtggt tcaggtaacg ggctatggca gtcgtggcga
     2821 acgctggatt attgatcgtt acaacatcac gcagtcattg cgcagtgact gcgacgggga
     2881 gagccagcga attgatccgg ccagctatcc ggaagactgg gatgtcctgc tgacggatgt
     2941 ttttcataaa agctggccgc tggcctccga tccttctcaa caaatgcgac tgatggcaat
     3001 ggcggtggac tccggcggtg aagacggggt cactgataat gcctataaat tctggcgtcg
     3061 ttgccgtcgt gatggccttg gtaaacgtat ttacctgttt aagggtgaca gcatccggcg
     3121 cgcaaaactg atcagccgta cattccctga taacaccgga cgaacgggcc gccgggcgca
     3181 ggccgcaggt gatgtgccgc tctggcttct tcagacggat gccctgaaag accgggtgaa
     3241 taacgcgtta tggcgtgact cgccaggtcc aggctatgtg catttccctg actggctggg
     3301 gagctggttt tacgacgaac tgacgtatga agagcggagc agtgacggga aatggagtaa
     3361 gccgggtcgc ggtgccaacg aagcttttga cctgatggtg tatgccgagg ctctggtcat
     3421 tctgcatgga tacgaaaaga tccgctggcc ggatgcaccg gagtgggcga gccgggagac
     3481 ctggctggag tgtgtccagg acagtaccga accgtcatcc tcaccggaac cggtatccac
     3541 gcctgttaaa aaacaaaaac ggaagaaaac agtaactgac gatgttaacc cctggctgac
     3601 ttccggagga tggttatgaa ccagaatgat atcgaagcca tgattcagcg ttatacggaa
     3661 gctgaaatgg cggtgctgga cggaaaatcc gtcaccttta atggtcagca gatgaccatg
     3721 gaaaacttat ctgagatccg gcagggacgg caggagtggg agcgccgcct tgcggctctg
     3781 attacacgac gacgggggca tcccgggtac cggctggcga ggttctgatg gcaattcttg
     3841 atgatgtgat tggcgttttt tcaccaggat ggaaagcggc aaggctgcgt tcccgtgcgg
     3901 tgatccaggc ttatgaggcc gtaaaaacga cgcggacaca caaagcccga cgggagaacc
     3961 gaactgccga ccagttaagc cagtacgggg ccgtgtcgtt acgtgagcag gcccgttacc
     4021 ttgataacaa ccacgatctg gttattggtg tatttgacaa gctggaagaa cgggtggtgg
     4081 ggaaaaacgg gattattgtc gagccacatc cggtattacg caatggggcc attgcccgtg
     4141 acctggctgc ggagattcgc acccgatgga gtgaatggtc tgtcagcccg gaagtcaccg
     4201 ggcagtttac ccgtccgatg ctggaacgtc tgatgcttcg tacctggctg cgcgatggtg
     4261 aggtgtttgc ccagatggtt tccgggcgca taaacagcct ggcgccttct gccggtgttc
     4321 atttctggct ggaggcgctc gagccggact ttattcccat gactagtgat gagagcaaca
     4381 ggctgaatca gggcgtgttt gttgatgact gggggcgtcc cgaaaaatat ctggtgtata
     4441 aaagccgtcc cgtatccgga cggcagatgg aaaccaaaga agtggatgca gagcgaatgc
     4501 tgcatcttaa atttgttcgc cgtctgcacc agatgcgcgg gacgtctttg ttgtccggtg
     4561 tgctgatccg cctcagtgcc ctgaaagaat atgaagattc tgagctgact gcagcaagga
     4621 tcgccgctgc tctggggatg tacatccgga aaggcgacgg gcagagctat gaaccggatg
     4681 gtaatggcag aaaggagaat gaacgtgagc ttaccattca gccgggcatt atttacgacg
     4741 atctgaaacc cggcgaagaa atcggaatgg tgaagtcgga tcgccccaat cctaaccttg
     4801 aaacttttcg taatggtcag ttgcgtgccg tggcggcggg cagtcgtctg agtttttcca
     4861 gtacagcgcg caactataac ggcacttaca gcgcccagcg tcaggagctg gttgaatcca
     4921 ctgatggcta cctgatcctg caggactggt ttattggtgc cgtcacccgc ccgatgtatc
     4981 gtgcatggct gaaacaggct gtggcatccg gtgttatcag gctaccccgc gatcttgacc
     5041 gttcttcact gtataccgcg gtgtattccg gaccagtgat gccgtggatt gaccctgtta
     5101 aggaggctga ggcctggaaa atccagattc gtggtggagc ggcgacagaa tcagactggg
     5161 tacgtgctgg tggtcgtaat ccggatgatg tcaaacgtcg gcgcaaggcc gaaattgatg
     5221 aaaaccgcaa gctggatctg gtatttgata ccgatccggc cagtgataaa ggaggcagta
     5281 gtgccgcaac gaaacgacag gagccgcagc acaccgacga ccagtccgaa gaataattcc
     5341 tggttcagga tgcaggctgg tcaccagagt gacgcggata tttatattta tgacgagatt
     5401 ggtttctggg gtgttacagc gaagcagttt atcagtgatc tgaatgcact gggcgatatc
     5461 acccacatta atctccatat taattcaccg ggtggcgatg tctttgaagg catcgccatt
     5521 tttaatgcgc tgaaaacaca tggtgcgtcc attaccgttt atgtcgacgg tgtggcggcg
     5581 tcaatggcgt cggtcattgc gatggtagga aacccggtca ttatgccgga aaactccttc
     5641 atgatgattc ataaaccatt tggctttacg ggcggtgatg cggaggacat gcgcacctat
     5701 gccgacctgc tcgataaagt tgaggcggtt ctgttacccg cttatgcaca gaaaaccggg
     5761 aaaaccaccg atgaaattgc tgccatgctg gcggatgaga cctggatgtc cggtgccgaa
     5821 tgtctggcac agggatttgc tgatcaggtg acaccagccg ttaaggcaat ggcatgtatt
     5881 cagtcaaaac gtacagagga atttaaaaag atgccggaat ccattcgaaa tatgattact
     5941 ccgccacgca acagtgctcc acgcgtacag gatgatggac ctgcagcctc ccggacgcca
     6001 gtgcaggcag cagcacccgt ggtggatgaa aacagcatcc gtgcgcaggt cctggcagag
     6061 caaaaagcgc gtgtaaacgg tattaatgat ctgtttgcca tgtttggcgg gcgttatcag
     6121 tcgttgcagg ctcagtgtct tgccgatcct gaatgttcgc tggagcaggc ccgcgaaaag
     6181 ctgttgaacg agatggggcg cgagtccacg ccatccaata aaaatacccc ggctcatatt
     6241 tatgccggta acggtaattt tgtgggggac gggatccgcc aggcgctgat ggcgcgcgcc
     6301 gggtttgaaa aaactgaacg tgataatgtc tacaacggga tgaccctgcg tgaatatgcc
     6361 cgtatgtcac tgactgaacg tggtattggt gtttccagtt ataacccgat gcagatggtc
     6421 ggtgcggcgt tcacacacag tacgtctgac ttcggtaata ttctgctgga tgttgcgaac
     6481 aaagccattc tgcagggctg ggaagatgcc cctgaaacct atgaacagtg gacgcggaaa
     6541 ggtcagttgt ctgattttaa aattgcccat cgtgtgggta tggggggctt cagtgctctg
     6601 cgtcaggtgc gtgaaggggc agaatataaa tacgtcacca ccggagataa acaggccact
     6661 attgcactgg cgacctatgg cgagctgttc agtatcaccc gtcaggccat tatcaatgat
     6721 gatctgaata tgctgaccga tgtcccgatg aagctgggcc gtgcggcgaa atccactatt
     6781 gccgatctgg tttatgccat tctgacgtct aacccgaaaa tctccacaga taatgtaagt
     6841 ctgttcgata aagcgaaaca tgcaaacgta ctggagagcg cggcaatgga cgtggcatcg
     6901 ctggataaag cccgccagtt gatgcgcgtt cagaaagagg gtgagcgtca tctgaatatt
     6961 cgtcctgcgt tcgtactggt accgacggcg atggagtctg ttgctaacca ggtcattcgc
     7021 tcctcaagtg tcaagggggc tgacattaac gccggtatta ttaacccggt gaaagatttt
     7081 gcgaccgtta ttgcagagcc tcgtcttgat gataacagcc agaccacctt ctacctggct
     7141 gcgtccaaag gctccgatac gattgaagtg gcttatctca acggtgtgga tacgccatat
     7201 attgatcaga tggagggctt cagtgtggat ggcgtgacaa cgaaagtgcg tattgacgct
     7261 ggtgttgcgc cagttgatca ccgcggcctg gtgaaatgta cggcgtaaac gtcgcagaca
     7321 acaactctga tggcccgtaa gggctttttt tgtacctgaa atcagcccct gaacggggct
     7381 gtgcggagac agttatggca aagaattttg tagaagaagg aaaaacggtg gcgattgttg
     7441 ccagtgcagc catcagcagc ggagatctgg tgcaggtggg ggatgttttt gcggtggcgc
     7501 tgaccgatat tccacaggga gaaacaggcg acggcataac cgaaggtgtg tttatgctgc
     7561 ctaagctgaa aacggatgac atgaaaacgg gtaagaaggt ttatctgaag tcaggaaaag
     7621 ttcagctgac taacagcggc tctgagccgc tggtcggggt tgtctgggca gatgccggaa
     7681 ccagtgcaga agaagtgccg gtaaaactca atgtctgatc ccttttcccg gctggcagcg
     7741 cgtatggatg cgatcacggt cagaaagatg ggaaagacag cctcgattaa tgatgtcgat
     7801 atgattgtga tcccgggaga aacactggca gagctgaatg ctctgtccgg acctgcggtc
     7861 tctctggtgg tgttttcttc gggataccgc ccacggcgcg gggatcgcgt tgtttatgac
     7921 ggacaacaat ggacggtcac acggcatgaa cgttttaacg gtaagccaat gatctttatt
     7981 gagtaaagag gtgtgggatg aaggggcttg agaatgccat ccgcaatctg aacagccttg
     8041 atacccgtat ggtgccacag gccagcgcat gggcgataaa ccgtgtggca cagaaagcgg
     8101 tctcggttgc cacccggcag gttgccggga ataccgttgc gggagataac caggtgaaag
     8161 ggatccccct gaaactggta cgtcagcgtg tccgggtgtt taaagccagt ccgtcaggaa
     8221 aaatgacggt caggatccgc gttaaccggg gcaatctgcc cgccattaag ctggggacag
     8281 cccgggtcag actggcccgg cgtggtggaa aactgcagta ccgtggcagt gtgctgaagg
     8341 tgggtaaata tcttttccgg gatgcgttta ttcagcaact ggcgaatggt cgctggcatg
     8401 tgatgcgacg tattgatggc aaaaatcgtt accccattga tgtggtgaaa atcccgctgt
     8461 ccggatcgct gacacaggca tttgaagatg cccgcgacca catcattgct gcggaaatgc
     8521 cgaaacagct ggggtatgca ctaaaacaac aactgaggtt atggctgacc cgatgaaccg
     8581 acatacacaa atccgccagg ccgtactggc acgccttcgg gaacagtgtg gagacagcgc
     8641 cacgtttttt gacgggcttc cggcatttat tgatgcgcag gaactgcctg ccgtggcggt
     8701 gtggctgagt gatgctcagt acaccggaaa aatgacggat gaagatgact ggcaggctgt
     8761 tctgcatatt gccgtcttca tccgggcaca ggcaccggat tcagagctgg atatgtggat
     8821 ggagagcacc attttccctg ccctgaatga tataccggca ctttccggac tcatcgacac
     8881 cctgatccca ctcggtttta actatcaacg tgataatgag atggccacct gggcgatggc
     8941 ggaaatcacg taccagatca cgtacacgaa ttaaggaggt ggtaatgacc acaccaaatc
     9001 cactggcaaa gacgaaaggt gcggggacga cgttctggat gtataccggc aacggcgatg
     9061 cgtttgcgaa ccctttgtcg gacactgact ggctgcgtct tgcgatggtg aaggatctgc
     9121 aacctggcga aatgaccgct gatgcagaag atgacactta tctcgatgat gaagatgcag
     9181 actggaaaac gacaacccag gggcagaaat ccgttggtga tacttcggcg acgctggcct
     9241 ggcgtccggg tgacagcggg cagaaaaaac tggttcagtt gttcgactcc ggtgaagtct
     9301 gcgcgtttcg tatcaaatat cccaacggca ctgttgatgt tttccgtggc tggctgagct
     9361 cactgggtaa aaccattgcc tcaaaagacg tgatgacccg cactgtgaaa atcagcggtg
     9421 tggggcgtcc gtatctggca gaggaaggca ctgaaaccgt gagcgttacc gggctgacag
     9481 tatcaccggc gtctgccagt gtaaaagtgg gagcaaccac cacgctgacc tttacagtaa
     9541 aacctgacgg agccagtgac aaagcgatca gtgtgcattc gtcagatcca cagactgcca
     9601 cggttaccct gaacggactt gtggccacgg taaaaggcgt gaagcagggc agtgtcagca
     9661 ttgtgggcat gaccgctgac gggaattttg tggctgtgac tgcggtgact gtcagcgcag
     9721 caggttaaca ggacgatact catcatttgc cccggttatt cggggctttt ttgcaggtgg
     9781 agaacatgat gtttctgaaa caggacacgt ttaattatga aaaacagtcc gtggtgctca
     9841 gtgagctgtc cgggctgcag agaattgaat atctgacgtt tgttcagcag cgaacggcaa
     9901 agtttgatgc cggggaggga gaactgccgg aggctgaacg acagattgct tttctgcgga
     9961 tggggatgga tatcaatgcc tggctggttt cccgctcact gtggaatgcg gatcagtcta
    10021 aggatgtaga gacgctttgc gcatccgtta ttacaacatg gtcgtatgat gccctgggtg
    10081 cgggggcgga gatggttctg tcgctgagcg gtatgggggc cattgataat gccgtggatg
    10141 atgagcatga ggcgctgacg ccggaaaagt cctgacgcgg gaaatgcagt ttgtcatgcg
    10201 gcttgcccgg gagttccggc gggcagactg gcggcggatg ctgtcggaaa tgtcggccac
    10261 tgagcttggt gagtggggcg attatttccg gatgcagagc ttcagtgatg tgtggatgga
    10321 tgcgcagttt gcctcgctga aggcattgat cgtgagaatg gtgtccggca gcagtgatgc
    10381 tgcggtggct gatttcagcc ttttaccgga agagaacggg ataccggagc gaacggacga
    10441 agaactgatg catcttgggg aaggtatttc cggaggtgtg cgttatggac cagatagcca
    10501 acctggtcat tgatttgggg attgatgcgg cagagtttaa aaatgaaatt ccccgtatca
    10561 aaaaccttct gaatggtgca gccagcgatg cagaacggtc ttctgcccgt atgcagcgtt
    10621 ttatggagcg tcagactcag gccgcccggc agacaacgca ggcagcttct tcggctgcaa
    10681 cagccgcatc cgtccatgcg cagacggtgg agaagaacgc acaggctcat gaacgcatgg
    10741 cccgcgaggt ggagaaaacc cgccagcgca tggaggcact gagccagaaa atgcgcgagg
    10801 aacaggcgca ggccatggct ctggcggagg ctcaggataa agcggctgct gcgttttatc
    10861 gtcagattga cagtgtgaaa caggccagtg cggggctgca ggaattacag cgtattcagc
    10921 agcagatccg acaggccaga aacagtggcg ggattggtca gcaggattat ctggcgctga
    10981 tttctgaggt tactgcgaaa acccgtgttc ttacacaggc tgaggaagag gctacccgac
    11041 agaaagtggc gtttatccgt cagcttaaag agcaggcaac ccgcctgaat ctttcatctt
    11101 ctgagttgct tcgtgctaag gcagcccagc tgggggtaag cagtgctgca gaagtgtata
    11161 tccgcaaaat ggagcaggca ggaaaagcca cgcattcgct gggtctgaaa agtgcagcag
    11221 cccgccagga gataggcgtt ctgataggtg aactggcccg cggcaattta ggggcgctga
    11281 ggggatccgg gataacgctg gctaaccgtg ccggatggat agacacactg atgtcaccga
    11341 aaggcatgat gcttggcggg gttattggcg gtattgccgc ggccgtctat ggtctgggta
    11401 aagcctggta tgacggtcag aaggaggggg aagaatttaa ccgccagttg tcgctgacgg
    11461 ggcattatgc cggagtcact gccgggcagc tgtggacgct cagtcgtgct atttccggga
    11521 atggtatcac gcaacatgct gcagccggtg cgctggctca ggtggtgggg agtggtgcat
    11581 ttcgtggaaa cgatatcggt atggtggcga gagttgccgc acagatggag cgatcggttg
    11641 gccagtcggt cagcgatacc ataaatcagt ttaagcggct gaaggatgat cctgtaaatg
    11701 ccgcgaaggc tctggacaat gagctgcatt ttcttactgc cactcagctt gagcagatac
    11761 gcgtccttgg ggagcagggg cggtccagtg atgctgcacg gatagccatg tctgcactgg
    11821 cagaggaaac cggtcggcgt actgcggata ttgataataa cctcaatgcg cttggcagta
    11881 cgctgaagta tctgtctgat ttgtggagtc gtttctggga tgcggccatg aatattggtc
    11941 gtgaagactc gctggatgaa cagattgccg ctttacagga gaaagtgtcg cgggcgaaaa
    12001 gactcccctg gacggcatca tcttctcagg ttgaatacga tcagcagcgt cttaacgatc
    12061 ttcaggagaa aaaacgccag aaggatttgc aggatgcaaa agagcaggca gagcggaatt
    12121 atcaggagca acagaaacgc cgtaatgctg aaaatgctgc actgaaccgg atgaatgaaa
    12181 cggaagcagc acgacatcag cgtgaaattg cgcgtattaa tgccatgcag tacgccgatc
    12241 aggctgtcag ggatgcggcg atacaacgtg aaaatgaacg ttacgagaaa gccctggcat
    12301 ccggtaagaa aaaaacacgc gaaacccgta atgatgaggc cacccggtta ttgctgcagt
    12361 acagtcagca acaggcacag gtggaaggac agattgctgc tgccagacag tcagcaggca
    12421 ttgccacgga aaggatgaca gaagcgcata aacagcttct ggctctgcag cagcgcatca
    12481 gcgacctgga cgggaaaaaa ctgacggcag atgaaaagag tgtgctggcc cgtaaagatg
    12541 aactgattca ggcactgacg ctgctggatg taaaacagca ggagcttcag aaacagacgg
    12601 cactcaacga gctgaagaaa aaaacaattc agctgaccag tcaactggct gaagaagagc
    12661 gcgctcagcg tcagcaacat gacctggata tcgccacggt gggtatgggt gatcagcagc
    12721 gacagcgata tcaggtacaa ctgagtcttc gccagaaata ccagcaacag ctggagcagt
    12781 tgaggcggga tagtgagcag aaagggacat ataacacgga tgactacaga aaggccgagc
    12841 aggcgctgac ggagagcctg aaccgacaac tgaatgagaa tcgccgttac tggcaacagc
    12901 ttgaagttgt gcagggtaac tggaaaaacg gagtcctgcg tgcatttcag gattttaccg
    12961 tggatgcaga taatacggca gaaacagcag aacaggtgtt ctcgtcagcc ttcagcaaca
    13021 tgggaaatgg cctggcaact tttgtcacta ccggcaaact caatttcaaa tccttcacct
    13081 cttctgtgct gtcagatatg gcgaaaatcc tggcgcaggc aaccatgatg aaatcgataa
    13141 aagggattgg cagtgtactg ggatttgatc tcagcagcct ttccctgaat gccaatgggg
    13201 ggatttatca gtctgctgat ttgagtcgtt acagtggcac ggtggttaac cgtccgacgt
    13261 tttttgcttt tgcaaaaggc gcgggtgtga tgggggaagc gggacctgaa gccattctgc
    13321 cattgcgtcg tggtgctgac ggtaagctgg gggttgtggc ggatattggg gggtcaggta
    13381 tggcgatgtt ttccccgcag tacaacatcg agatcaataa cgatggcacg aacgggcaga
    13441 taggtccggc tgccctgaag gcggtttatg acctcgggaa aaaagcggca gcggacttta
    13501 tgcaacagca ggcccgtgat ggtggtcggt taagtggagc atatcggtaa tggagacgtt
    13561 tcactggaaa gtgcgcccgg atatgaatgt ggtatcagag ccgaaagtgg tgacagtgaa
    13621 gctgggcgat ggttatgaac agcgtcgtgc ggcgggactg aataaccagt tgtcgactta
    13681 cagcgtgacg atacgtgttc gtaaatgtga ccacccatct ttaaaagcct ttctggaacg
    13741 gcacggtggc gtccgtgcat ttcagtggac gccaccttat gactggaaac cgatcagggt
    13801 ggtttgtcgt aaatggtcgg caagcgtggg ggcgctgtgg gtaaccataa cggcagattt
    13861 tgaacaggtc gtggcatagg aggctctgat gcaggatatt ccacaggaaa cacatcatga
    13921 gacgacacgc ctcactcagt cagcccaggt ggtgctctgg gaaatcgatc tgacagaggt
    13981 cggtggtgaa cgttattttt tctgtaatga gcagaacgaa aaaggtgagc cggttatctg
    14041 gcaggggcgg cagtatcagg tataccccat tcaggggacg ggatttgaac tgaacggcaa
    14101 gggcagtgct gcccgtccga cactgacggt ttctaacctg tacggtatgg tcaccgggat
    14161 ggcggaagat ctgcagagtc tggtcggcgg aacggtggtc cggcgtaagg tttacgcccg
    14221 ttttctggat gcggtgaact tcgtcaacgg aaacagtgac gccgatccgg agcaggaggt
    14281 gatcagccgc tggcgcattg agcagtgcag cgaactgagc gcggtgagtg cctcttttgt
    14341 actgtccacg ccgacggaaa cggacggcgc tgtttttccg ggacgtatca tgctggccaa
    14401 cacctgcacc tggacctatc gcggtgatga gtgcggttat cacggtccgg cagtcgcgga
    14461 tgaatatgac cagccgacgt ccgatatcac gaaggataaa tgcagcaaat gcctgagcgg
    14521 ctgtaagttt cgcaataacg tcggcaactt tggcggcttc ctttccatta acaaactttc
    14581 gcagtaatcc catgacagag acagaatcag cgattctggc gcacgcccgg cgatgtgcgc
    14641 cagcggagtc gtgcggcttc gtggtgagag cgccggaggg ggaaagatat tttccctgcg
    14701 tgaatatttc cggtgagccg gaggattatt tccggatggc tccggaggac tggctgcagg
    14761 cagagatgca gggtgagatt gtggcactgg tccacagtca tcccggtggt ctgccctggc
    14821 tgagtgaggc tgaccggcgg ctgcaggtgc agagtgattt gccgtggtgg ctggtctgcc
    14881 ggggggcgat tcacaagttc cgctgtgtgc cgcatctcac cgggcggcgc tttgagcacg
    14941 gggtgacgga ctgttacacg ctgttccggg atgcttatca tctggcgggg attgagatgc
    15001 cggattttca tcgcggggat gactggtggc gtaacggtca gaatctctat ctggataatc
    15061 tggaggccac agggctgtat caggtggcgt tgtcatcagc acaaccgggc gatgtgctgc
    15121 tgtgctgttt tggttcatcg gtgccgaatc atgccgccat ttactgcggc gacggtgagc
    15181 tgctgcacca tattcctgaa caactgagta aacgagagag gtataccgac aaatggcagc
    15241 gacgcacaca ctccctctgg cgtcaccggg catggcacgc atctgccttt acggggattt
    15301 acaacgattt ggccgccgca tcgaccttcg agtgaaaacg gggtctgaag ccatccgggc
    15361 gctggccatg cagatcccgg cgtttcgtca gaaactgagc gacggctggt accaggtacg
    15421 cattgccggg cgtgatgcag gtgaaaccga attgtctgcc cgtcttaatg agccgctggc
    15481 aaatggtgcc gtgatccaca tcgtgccgcg tctggcggga gctaaaagtg gcggtgtgtt
    15541 tcaggtggtg ctgggggcgg cgctgattgc ggtggcatgg tggaaccctg tgggctggct
    15601 gggtgccgcg gctgtatcgg gcatgtatgc ggcaggggcc agtatgatcc tgggcggagt
    15661 ggcgcagatg ctggcaccga aagccaggac gcccacggca gcaagtacag ataacggcaa
    15721 acagaacacc tatttctcct cactggataa catggttgcc cagggcaatg ttctgcccgt
    15781 tctgtacggt gaaatgcgcg tggggtcgcg ggtggtctct caggagatca gcacggcaga
    15841 cgaaggggat ggtggtcagg ttgtagtgat tggtcgctga tgaaaaacgt tttatgtgaa
    15901 accgcctccg ggcggttttg tcgtttatgg agcgttagga atgggtaaag gcagcagtaa
    15961 ggggcatact ccgcgcgaag cgaaggacaa cctgaagtcc acgcagttgc tgagtgtgat
    16021 cgatgccatc agcgaagggc cgattgaagg tccggtggat ggattaaaaa gcgtgctgct
    16081 gaacagtacg ccagtgctgg acactgaggg gaataccaac atctccggcg tcacggtggt
    16141 gttccgggcc ggtgagcagg agcagacacc gccggaggga tttgaatcct ccggatccga
    16201 gacggtgctg gggacggaag tgaaatacga cacgccgatc acccggacca tcacgtcggc
    16261 aaacatcgac cgtctgcgct ttaccttcgg tgtgcaggca ctggtggaaa ccacctcaaa
    16321 gggggacagg aatccatcgg aagtccgcct gctggttcag atacaacgta acggtggctg
    16381 ggtgacggaa aaagacatca ccattaaagg caaaaccacc tcgcagtatc tggcctctgt
    16441 ggtggtggat aacctgccgc cgcgcccgtt cagtatccgg atgcgcagga tgacgccgga
    16501 cagcaccaca gaccagctgc agaacaaaac gctctggtcg tcatacaccg aaatcatcga
    16561 tgtgaaacag tgctacccga acacggcact ggtcggcgta caggtggatt cggagcagtt
    16621 cggcagccag caggtgagcc gtaattatca tctgcgcggg cgtattctgc aggtgccgtc
    16681 gaattataac ccgcagacgc gacaatacag cggtatctgg gacggaacgt taaaaccggc
    16741 atacagcaac aacatggcct ggtgtctgtg ggatatgctt acccatccgc gctacggcat
    16801 ggggaaacgt cttggtgcgg cggatgtgga taaatgggcg ctgtatgtca tcggccagaa
    16861 ttgcgaccag tcagtgccgg acggctttgg cggcacggag ccgcgcatca cctgtaatgc
    16921 gtacctgacc acacagcgca aggcgtggga cgttctcagt gatttctgct cggcgatgcg
    16981 ctgtatgccg gtatggaacg ggcagacgct gacgttcgtg cagaaccgac cgtcggataa
    17041 ggcgtggacc tataaccgca gtaatgtggt gatgccggat gatggcgcgc cgttccgcta
    17101 cagcttcagc gccctgaaag accgccataa tgccgttgag gtgaactgga ttgacccgaa
    17161 caacggctgg gagacggcga cagagcttgt tgaagatacg caggccattg cccgttacgg
    17221 tcgtaatgtt acgaagatgg atgcctttgg ctgtaccagc cgggggcagg cacaccgcgc
    17281 cgggctgtgg ctgattaaaa cggagctgct ggagacgcag accgtggatt tcagcgtcgg
    17341 cgcagaaggg cttcgccatg taccgggcga tgttattgaa atctgcgatg atgactatgc
    17401 cggtatcagc accggtggtc gtgtgctggc ggtgaacagc cagacccgga cgctgacgct
    17461 cgaccgtgaa atcacgctgc catcctccgg taccacgctg ataagcctgg ttgacggaag
    17521 tggcaatccg gtcagcgtgg aggttcagtc cgtcaccgac ggcgtgaagg taaaagtgag
    17581 ccgtgttcct gagggtgttg ctgaatacag cgtatggggg ctgaagctgc cgacgctgcg
//
LOCUS       DAZGJW010000093        17450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZGJW010000093 DAZGJW010000000
VERSION     DAZGJW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.093"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(10..1437)
                     /gene="djlB"
                     /locus_tag="ACHKIK_003210"
     CDS             complement(10..1437)
                     /gene="djlB"
                     /locus_tag="ACHKIK_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO9084331.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1434..2141)
                     /gene="ybeR"
                     /locus_tag="ACHKIK_003211"
     CDS             complement(1434..2141)
                     /gene="ybeR"
                     /locus_tag="ACHKIK_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO9084332.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            2305..3282
                     /gene="ybeQ"
                     /locus_tag="ACHKIK_003212"
     CDS             2305..3282
                     /gene="ybeQ"
                     /locus_tag="ACHKIK_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFO9084333.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3352..3834)
                     /gene="ybeL"
                     /locus_tag="ACHKIK_003213"
     CDS             complement(3352..3834)
                     /gene="ybeL"
                     /locus_tag="ACHKIK_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO9084334.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4069..6651
                     /gene="leuS"
                     /locus_tag="ACHKIK_003214"
     CDS             4069..6651
                     /gene="leuS"
                     /locus_tag="ACHKIK_003214"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO9084335.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            6666..7247
                     /gene="lptE"
                     /locus_tag="ACHKIK_003215"
     CDS             6666..7247
                     /gene="lptE"
                     /locus_tag="ACHKIK_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO9084336.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7247..8278
                     /gene="holA"
                     /locus_tag="ACHKIK_003216"
     CDS             7247..8278
                     /gene="holA"
                     /locus_tag="ACHKIK_003216"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO9084337.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8280..8921
                     /gene="nadD"
                     /locus_tag="ACHKIK_003217"
     CDS             8280..8921
                     /gene="nadD"
                     /locus_tag="ACHKIK_003217"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO9084338.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            8945..9556
                     /gene="cobC"
                     /locus_tag="ACHKIK_003218"
     CDS             8945..9556
                     /gene="cobC"
                     /locus_tag="ACHKIK_003218"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO9084339.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            9816..10133
                     /gene="rsfS"
                     /locus_tag="ACHKIK_003219"
     CDS             9816..10133
                     /gene="rsfS"
                     /locus_tag="ACHKIK_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO9084340.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10137..10604
                     /gene="rlmH"
                     /locus_tag="ACHKIK_003220"
     CDS             10137..10604
                     /gene="rlmH"
                     /locus_tag="ACHKIK_003220"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO9084341.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            10635..12536
                     /gene="mrdA"
                     /locus_tag="ACHKIK_003221"
     CDS             10635..12536
                     /gene="mrdA"
                     /locus_tag="ACHKIK_003221"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO9084342.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12539..13651
                     /gene="mrdB"
                     /locus_tag="ACHKIK_003222"
                     /gene_synonym="rodA"
     CDS             12539..13651
                     /gene="mrdB"
                     /locus_tag="ACHKIK_003222"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO9084343.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            13662..14750
                     /gene="rlpA"
                     /locus_tag="ACHKIK_003223"
     CDS             13662..14750
                     /gene="rlpA"
                     /locus_tag="ACHKIK_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO9084344.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            14890..16101
                     /gene="dacA"
                     /locus_tag="ACHKIK_003224"
     CDS             14890..16101
                     /gene="dacA"
                     /locus_tag="ACHKIK_003224"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO9084345.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16212..16475
                     /gene="ybeD"
                     /locus_tag="ACHKIK_003225"
     CDS             16212..16475
                     /gene="ybeD"
                     /locus_tag="ACHKIK_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO9084346.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            16576..17217
                     /gene="lipB"
                     /locus_tag="ACHKIK_003226"
     CDS             16576..17217
                     /gene="lipB"
                     /locus_tag="ACHKIK_003226"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO9084347.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         4399 a         4392 c         4451 g         4208 t
ORIGIN      
        1 cgtcgactgt caaccgaaca gatggagtat tttccctatc aaaccagcaa agataaaaat
       61 atagaaaata attttaatga tgcccagaca gcctggccca ccatcgtcaa cgggcattgg
      121 ctgcggttgc ggctccggtt caacagtttg cttctcggta aaattcgccg cctgaattaa
      181 taatcgacga ataattaatg cttccagtgg acaacgatct tctgcatcaa ggcggctcac
      241 aatcggcatt aatgattctt cggttggttg gttcgcctgc actaattgtt tggcaactaa
      301 atataatggc cataagggat gttctgtatc acgcgtgagt gagatccgcg gcttacgtcc
      361 tttgtgcaaa accatctcga ccagtgaacg ccccagagca gagagttttt catcgctgag
      421 tatttcgtat aaatgaaaac gcgtacgggc gctgtcgccg gggatataaa gtagcggttt
      481 tccctgatcg ttaaccagtt cacgagcttc aatcatcatc accactaacg ggtaataatc
      541 gacccgatgc tgacggcacc attgcaatat taaagcatcc gcctgggtgg agggatactc
      601 gcgccagtag tcacaaagtt ctgccagcaa gctttcgcct tctccacaat agacgcgttg
      661 tgcgtattca taataacgcg catcttcatt atcagattca gcttcccgcc acgaccgggc
      721 gtaatccagg agttcgggaa ttcccaggcg acctgcgcta taccagcgga gtaaatttct
      781 gtgcagtttt gcatcatcgg ggataatcac tgcgcgatgt tgcgctaaat acaatgtcag
      841 ccattctgga tgcgatgacc aaatccgagc gaacatttgg taaaaatcga tggtgtcatt
      901 ttgcacggca acgggcagat gcagaatggt ctggaagtta aaaacattcc ccttcgcaat
      961 ggcatataaa aaggattcca gttcttcttc gtctatttcc ccgacgtttt cctcttgcaa
     1021 ccaccgcaat ctttctgcta ataacaccac gcactcgaag gataaatggg cagtgttcat
     1081 ggcgattgtg cacagcgacc agcgtaattc atcaatctct tccatcgagc aataatttaa
     1141 ttgctgaatg aatcgctgcc aggcggaggg cagaaaacgt tcgctatcag aggcaagtaa
     1201 cgcacgaaag gcgagcagaa tttcatgttc tgctacctca tattcttctg gttgccaaac
     1261 agatttagca ggcgactgcg caatccgtag cgcctcctca tacgcctgac gaagttgttt
     1321 aaaaccctgc ggatcggttt ctggatgaaa ggacggtaac agcgccagat aagcgcggcg
     1381 gataatatcg acgtcagtcg tttcctctat ttcgagaatc ttccagcaat ttttcatagg
     1441 tcgctcatat ccttaagcgt ttccgggcat gcagaatagt aagccaatgg ttgccaggac
     1501 aaggtagggt aatacgcatc atcactggca aataattgat aaaacatatc attgccggaa
     1561 tcgtcgtatt ctttacgcgc taagaattcg cgtaataact gcagatgatc atcttcggga
     1621 gactgccaat attgccgacc aaaggtataa ccggcaaagt attgccgcca gttacgataa
     1681 taacgtagag cccgtagatg aatgcgcgac tggatccata gactttcttc ttcgctcaac
     1741 cagttattaa gtacgcccat tcggcttaaa aaacccatcc ggacataatc ccaggcttta
     1801 attcctccct caccacagca ttcggcggtg ctggcgacaa attgcgcata agcctgacct
     1861 tgctcattta gttcagcaag atagtcacgc cattcacatg gcgaataacg aaaccagcgg
     1921 tgataaaacc ctgccaaccg agcggcatga ccgttatcga tcattcggtg aattgtgcgc
     1981 agcaggtcat cacgggcatc aattccccag gaggaagaaa ggtcaaaaaa gcatgtacct
     2041 aaatacattt tgggtgatga atgcgaaggt aaacagcatt cattgcgtat ttccatcgga
     2101 gtggaaagtg caaacaatat tttttgcgac tccatgtcca tataatttat ccttaaatat
     2161 tcattaaatg aatattagcc gcattgcgag gctcgtttat ttatactttt ctgacgcgac
     2221 ataatacgca taaaataagg cttacgcaaa aatggaacac ataaaaatat aatatgtttt
     2281 atctgcaacc ataaggaaat gataatgatt ttcacgtcaa gttgctgcga taatttatca
     2341 atagatgaga ttatcgaacg tgctgaaaaa ggcgattgcg aggctcaata tattgttggg
     2401 ttttattata atcgcgatag cgcaattgat tctccggacg acgaaaaagc cttttactgg
     2461 ctgaagctgg ccgctgagca aggtcattgt gaagcacagt attccttagg gcaaaagtat
     2521 accgaggata aaagccgtca taaagataat gagcaagcca tcttctggct gaaaaaagct
     2581 gccctacaag gccatacttt cgcttccaac gcccttggct ggacactgga tcgtggagaa
     2641 gcccccaatt ataaagaagc ggttgtctgg tatcagatag ccgcggagag cggaatgtct
     2701 tatgcgcaaa ataatcttgg gtggatgtac agaaatggca acggagtcgc aaaagactat
     2761 gcgctggcat ttttctggta caaacaagct gcattacaag gccatagtga cgcgcaaaac
     2821 aatctggccg atctttatga agacggaaaa ggcgttgctc aaaacaagac actcgccgca
     2881 ttctggtatt tgaaaagcgc acagcagggt aatcggcacg cccagtttca aattgcatgg
     2941 gattataacg ctggcgaagg ggtggaccag gactataagc aagcgatgta ctggtatctg
     3001 aaggctgccg ctcaggaaag cgtcggcgct tacgtcaaca tcggttatat gtataaacac
     3061 ggacaaggcg ttgagaaaga ttatcaggct gcctttgaat ggtttacgaa agccgctgaa
     3121 tgcaatgacg ccactgcctg gtataacctg gccattatgt atcactacgg agaaggaaga
     3181 cctgtcgatc tccgacaggc tctcgacctg tatcgtaaag ttcagtcatc cggaaccaga
     3241 gatgtcagtc aggaaattcg tgagactgaa gatttactgt agagataaaa acaagggccg
     3301 gggaatttca ttcggcccat attctcttta ttgctgtgcg aaatcagact attacggctc
     3361 aaacgggcgt ctctggaact ggtcatgacc acatttcggg catagcgtca gcacttccgg
     3421 tgtgtagatc gggagatgga agtgacattt ctcgcagacc agatttccca gcccgaccac
     3481 ttctccgctg tgataaaccc catgatgatt gaggtcctgg aaaacttcgc gccattcaag
     3541 ctgcgtttta tcggtgatat ctgccagctc ctgccataag ctttctttaa tcacccgcat
     3601 aaagacgctg tcagattctt ctttcaggct ctcttcatag ctcatggcga actcttccag
     3661 gtcacgtctt acagctcgcg tcagctcatc gacctcggtt cgcgttaact cccctgtttt
     3721 tattacgcgc tcgcgcgcct gttccaccag tgcgtcgata tcacgttcgc cattgcgcag
     3781 gcgttcgctc agtgacgcga ccagttcacg gtaatattga gcaaccttgt tcatcgtttc
     3841 atctcccggg tcgttaactg actataataa tagacgttct ttgttccttg ctttgctaat
     3901 acgcccacaa actctgtaaa ttaatgtatg aataatccgg gcatcatttc aacgtcgagt
     3961 gcgcgaaagg ctgttttgac gcggacgttt gggctatgct atgcggatct gaaaaaccac
     4021 ctcaacgcta catttgtagc cgtattgaaa acaggaccac tggctgccat gcaagagcaa
     4081 taccgcccgg aagagataga atccaaagta cagcttcatt gggatgagaa gcgcacattt
     4141 gaagtaaccg aagacgagag caaagagaag tattactgcc tgtctatgct tccctatcct
     4201 tctggtcgac tacacatggg ccacgtacgt aactacacca tcggtgacgt gatcgcccgc
     4261 taccagcgta tgctgggcaa aaacgtcctg cagccgatcg gctgggacgc gtttggtctg
     4321 cctgcggaag gcgcggcggt gaaaaacaac accgctccgg caccgtggac gtacgacaac
     4381 atcgcgtata tgaaaaacca gctcaaaatg ctgggctttg gttatgactg gagccgcgag
     4441 ctggcaacct gtacgccgga atactaccgt tgggaacaga aattcttcac cgagctgtat
     4501 aaaaaaggcc tggtatataa gaagacttct gcggtcaact ggtgcccgaa cgaccagacc
     4561 gtactggcga acgaacaagt tatcgacggc tgctgctggc gctgcgatac caaagttgaa
     4621 cgtaaagaga tcccgcagtg gtttatcaaa atcactgctt acgctgacga gctgctcaac
     4681 gatctggata aactggatca ctggccagac accgttaaaa ccatgcagcg taactggatc
     4741 ggtcgttccg aaggcgtgga gatcaccttc aacgttaacg actatgacaa cacgctgacc
     4801 gtttacacta cccgcccgga cacctttatg ggttgtacct acctggcggt agctgcgggt
     4861 catccgctgg cgcagaaagc ggcggaaaat aatcctgaac tggcggcctt tattgacgaa
     4921 tgccgtaaca ccaaagttgc cgaagctgaa atggcgacga tggagaaaaa aggcgtcgat
     4981 actggcttta aagctgttca cccattaacg ggcgaagaaa ttcccgtttg ggcagcaaac
     5041 ttcgtattga tggagtacgg cacgggcgca gttatggcgg ttccgggtca cgaccagcgc
     5101 gactacgagt ttgcctctaa atacggcctg aacatcaaac cagttatcct ggctgctgac
     5161 ggctctgagc cagatctttc tcagcaagcc ctgactgaaa aaggcgtgct gttcaactct
     5221 ggcgaattca acggtcttga ccatgaagcg gccttcaacg ccatcgccga taaactgact
     5281 gcgatgggcg ttggcgagcg taaagtgaac taccgcctgc gcgactgggg tgtttctcgt
     5341 cagcgttact ggggcgcgcc gattccgatg gtgacgctgg aagacggtac cgtaatgccg
     5401 accccggacg accagctgcc ggtgatcctg ccggaagatg tggtgatgga cggcattacc
     5461 agcccgatta aagcagatcc ggagtgggca aaaactaccg ttaacggtat gccagcgctg
     5521 cgtgaaaccg acactttcga cacctttatg gagtcctcct ggtactatgc gcgctacact
     5581 tgcccagagt acaaagaagg tatgctggat tccgaagcgg ctaactactg gctgccggtg
     5641 gatatctaca ttggtggtat cgaacacgcc attatgcacc tgctctactt ccgcttcttc
     5701 cacaaactga tgcgtgatgc aggcatggtg aactctgacg aaccagcgaa acagttgctg
     5761 tgtcagggta tggtgctggc agatgccttc tactatgttg gcgaaaacgg cgaacgtaac
     5821 tgggtttccc cggttgatgc tatcgttgaa cgtgacgaga aaggccgtat cgtgaaagcg
     5881 aaagatgcgg caggccatga actggtttat accggcatga gcaaaatgtc caagtcgaag
     5941 aacaacggta tcgacccgca ggtgatggtt gaacgttacg gcgcggacac cgttcgtctg
     6001 tttatgatgt ttgcttctcc ggctgatatg actctcgaat ggcaggaatc cggtgtggaa
     6061 ggggctaacc gcttcctgaa acgtgtctgg aaactggttt acgagcacac agcaaaaggt
     6121 gatgttgcgg cactgaacgt tgatgcgctg actgaagatc agaaagcgct gcgtcgcgat
     6181 gtgcataaaa cgatcgctaa agtgaccgat gatatcggcc gtcgtcagac cttcaacacc
     6241 gcaattgcgg cgattatgga gctgatgaac aaactggcga aagcaccaac cgatggcgag
     6301 caggatcgcg ctctgatgca ggaagcgctg ctggccgttg tccgtatgct taacccgttc
     6361 accccgcaca tctgcttcac gctgtggcag gaactgaaag gcgaaggcga tatcgacaac
     6421 gcgccgtggc cggttgctga cgaaaaagcg atggtggaag actccacgct ggtcgtggtg
     6481 caggttaacg gtaaagtccg tgccaaaatc accgttccgg tggacgcaac ggaagaacag
     6541 gttcgcgaac gtgctggcca ggaacatctg gtagcaaaat atcttgatgg cgttaccgtg
     6601 cgtaaagtga tttacgtacc aggtaaactc ctcaatctgg tcgttggcta agcgcgggag
     6661 gaagcgtgcg atatctggca acattgttgt tatctctggc tgtgttaatc accgccgggt
     6721 gtggctggca tctgcgtgat accacgcagg ttccttccac tatgaaggtc atgatcctgg
     6781 actcaggcga tccgaacggg ccattaagcc gtgcggtgcg taaccagtta cgtctgaatg
     6841 gtgtcgagtt gcttgataaa gaaaccacgc gtaaggacgt tccatccctg cgtttgggta
     6901 aagtgagcat cgcgaaagat accgcatcgg tattccgtaa cggtcaaaca gcagagtatc
     6961 agatgatcat gacggtgaat gcgaccgtgt tgatccccgg ccgtgatatc tacccgatta
     7021 gtgccaaagt cttccgttcg ttctttgata acccgcaaat ggcgttagcg aaagataacg
     7081 aacaagacat gatcgtaaaa gagatgtacg accgtgctgc cgaacagctg attcgtaagc
     7141 tgccaagcat ccgtgctgcg gatattcgtt ccgacgaaga acagacgtcg accacaacgg
     7201 atactccggc aacgcctgca cgcgtctcca ccacgctggg taactgatga ttcggttgta
     7261 cccggaacaa ctccgcgcgc agctcaatga agggctgcgc gcggcgtatc ttttacttgg
     7321 taacgatcct ctgttattgc aggaaagcca ggacgctgtt cgtcaggtag ctgcggcaca
     7381 aggattcgaa gaacaccaca ctttttccat tgatcccaac actgactgga atgcgatctt
     7441 ttcgttatgc caggctatga gtctgtttgc cagtcgacaa acgctattgc tgttgttacc
     7501 agaaaacgga ccgaatgcgg cgatcaatga acaacttctc acactcaccg gacttctgca
     7561 tgacgacctg ctgttgatcg tccgcggtaa taaattaagc aaagcgcaag aaaatgccgc
     7621 ctggtttact gctcttgcga atcgcagcgt gcaggtgacc tgtcagacac cggagcaggc
     7681 tcagcttccc cgctgggttg ctgcgcgcgc aaaacagctc aacttagaac tggatgacgc
     7741 tgcaaatcag gtgctctgct actgttatga aggtaacctg ctggcgctgg ctcaggcact
     7801 ggagcgttta tcgctgctct ggccagacgg caaattgaca ttaccgcgcg ttgaacaggc
     7861 ggtgaatgat gccgcgcatt tcaccccttt tcattgggtt gatgctttgt tgatgggaaa
     7921 aagtaagcgc gcgttgcata ttcttcagca actgcgtctg gaaggcagcg aaccggttat
     7981 tttgttgcgc acattacaac gtgaactgtt gttactggtt aacctgaaac gccagtctgc
     8041 ccatacgcca ctgcgtgcgt tgtttgataa gcatcgggta tggcagaacc gccggggcat
     8101 gatgggcgag gcgttaaatc gcttaagtca gacgcagtta cgtcaggccg tgcaactcct
     8161 gacacgaacg gaactcaccc tcaaacaaga ttacggtcag tcagtgtggg cagagctgga
     8221 agggttatct cttctgttgt gccataaacc cctggcggac gtatttatcg acggttgata
     8281 tgaaatcttt acaggctctg tttggcggca cctttgatcc ggtgcactat ggtcatctta
     8341 aacccgtgga aacgctggcg aatttgattg gtctgacgcg ggtcacaatc atccctaata
     8401 atgttcctcc gcatcgtccc cagccggaag cgaacagcgt gcagcgtaaa cacatgcttg
     8461 aactggcgat tgccgacaag ccattattta ctcttgatga acgcgagcta aagcgcaatg
     8521 ccccctctta cactgcgcaa acactgaaag agtggcggca ggaacaaggg ccggacgtgc
     8581 cgctggcgtt tattattggt caggattcat tgctgacctt tccgacctgg tacgaatacg
     8641 aaacgatact cgacaatgca catttgatcg tctgtcggcg tccaggttac ccacttgaaa
     8701 tggcgcaacc gcaataccag caatggctgg aagatcattt gacacataac ccggaagatc
     8761 ttcaccttca gcctgccggt aaaatttatc tggctgaaac gccgtggttt aacatctcgg
     8821 cgaccatcat ccgcgaacgt ttgcaaaacg gtgaatcgtg tgaggattta ttgccggaac
     8881 cggtactgac ttacattaac caacaaggct tgtatcgctg atacctgtcg ttacggaggg
     8941 catgatgcga ctgtggttaa ttcgtcatgg tgaaacgcaa gcgaatatcg atggtcttta
     9001 cagcggtcat gcgcccaccc ccctgaccgc gcgcggtatt gagcaagcgc aaaatctgca
     9061 tacactgcta gatgatgttt cctttgacct ggttttatgc agtgaactgg aacgggcaca
     9121 gcataccgcg cgacgggttc tcagtgaccg ccagctcccc gtgcaaatca tccctgaact
     9181 caacgaaatg ttttttggcg actgggagat gcgacatcat cgcgacctca tgcaagaaga
     9241 tgccgaaaac tatagcgcgt ggtgcaatga ctggcagcat gctatcccca cgaacggtga
     9301 aggatttcag gcattttcgc aacgtgtgga aagatttatc gcaaggctta gtgaatatca
     9361 gcactatcag aatattttaa tcgtcagcca tcagggcgta ctgagtctgt taattgctcg
     9421 tctaattggc atgcctgccg aatctatgtg gcattttcgc gttgaccagg ggtgttggag
     9481 cgccattgat atcaaccaaa aattcgcgac gctacgcgtc ctcaatagcc gtgccatcgg
     9541 ggtcgagaat gcatgacttt tctgtttttt tacgggtaag ccgcaacgac cattgacagc
     9601 cccgggcagg ctgatattct ccgcagccag actttttccg ccagacacga ctttgtagaa
     9661 attgttttac aaaaatggcg atgcaatctg cggcgcgggg tgggatgata gcccactttc
     9721 gaaagccgat tcggcgacaa ttgtcccgaa atcgcctctg gttcaggtat actgacagac
     9781 catttttatc tatttgattc acccaggggg aaaacttgca gggtaaagca ctccaggatt
     9841 ttgttatcga caaaattgat gacctcaaag gtcaggacat catcgcctta gacgttcagg
     9901 gcaaatccag catcaccgac tgcatgatca tctgtacggg tacgtccagc cgtcatgtta
     9961 tgtccattgc tgaccacgtt gtgcaggagt ctcgcgcagc gggcctgtta ccgctcggcg
    10021 tagaaggtga aaacagcgcc gactggattg tcgtggattt gggcgatgtg attgtccatg
    10081 tcatgcagga agagagccgt cgcctgtatg aactggaaaa actctggagt taatgcgtga
    10141 agctgcaact tgtcgccgtg ggaacgaaaa tgccggactg ggtacaaacc ggttttaccg
    10201 agtacctgcg tcgttttccg aaagatatgc ccttcgagct gattgaaatt ccggccggaa
    10261 aacgcggcaa gaatgcggac atcaagcgca tactcgacaa agagggtgag cagatgttgg
    10321 cggccgcagg caaaaaccgc attgtcaccc tcgatattcc aggcaagccc tgggatacgc
    10381 cgcagttagc cgctgagctg gaacgctgga agctggatgg tcgcgacgtc agtctactga
    10441 ttggcgggcc tgaagggttg tcgcctgcct gtaaagcggc ggcggagcag agctggtcgc
    10501 tgtcggcgct taccctcccc catccgctgg ttcgcgtgct ggtcgcagag agtctgtacc
    10561 gggcgtggag catcaccacc aaccatcctt atcaccgtga gtgataaagg agctttgagt
    10621 agaaaacgca gcggatgaaa ctacagaact cttttcgcga ctatacggca gagtccgcgc
    10681 tgtttgtgcg ccgggcgctg gtcgcctttt tggggatttt gctgctgacc ggcgtgctta
    10741 tcgccaacct gtataatctg caaattgttc gctttaccga ttaccagacc cgctctaatg
    10801 aaaaccgcat taagctggtg cctatcgcgc ccagccgcgg cattatctac gaccgtaacg
    10861 gtatccctct ggcactcaac cgcactatct accagataga aatgatgccg gagaaagtcg
    10921 ataacgtgca gcaaacgctg gacgctttgc gcagcgtggt tgatctgacc gatgacgata
    10981 ttgctgcatt ccgaaaagag cgcgcacgtt cacaccgttt cacctctatt cccgtgaaaa
    11041 ctaacctgac cgaagtacaa gtagctcgct ttgccgtcaa tcagtaccgt tttccgggtg
    11101 tcgaagttaa aggctataaa cgtcgttact atccttacgg ttcggcgttg acccacgtca
    11161 tcggctatgt gtcgaaaatc aacgataaag acgtcgaacg cctgaataat gacggcaaac
    11221 tggccaacta tgcggcaacg catgatatcg gtaagctggg cattgagcgt tactatgaag
    11281 acgtgctgca cggtcagacc ggttatgaag aggttgaagt taacaaccgt gggcgtgtta
    11341 tccgccagtt aaaagaagta ccaccgcaag ccggacacga tatttacctg acgctggatc
    11401 tcaaactcca gcaatatatt gaaacgctgc tggcgggtag ccgcgcagct gtggtagtca
    11461 ccgatccgcg tacaggtggg gtgctggcac tggtttccac gcctagttat gacccaaacc
    11521 tgtttgttga cggtatctcc agcaaagatt attccgcctt gctgaacgac ccgaatacac
    11581 cgctggtgaa ccgcgccaca cagggggttt atcctcctgc gtctacagtt aaaccctatg
    11641 tggcggtttc ggcattaagc gccggggtga tcacgcgcaa tacgacgctg tttgacccag
    11701 gctggtggca actaccaggg tcggaaaaac gttatcgtga ctggaaaaaa tggggccacg
    11761 ggcgtctgaa tgtcacaaga tcgctggaag aatctgcgga taccttcttc tatcaggtgg
    11821 cctacgatat ggggatcgat cgcctctccg aatggatggg taaattcggt tatggtcatt
    11881 acaccggtat cgacctggcg gaagaacgtt ccggcaacat gcctacccgc gaatggaaac
    11941 agaaacgctt taaaaaaccg tggtatcagg gtgacaccat tccggttggt atcggccagg
    12001 gttactggac agcgacccca atccagatga gtaaggcact gatgatcctg attaatgacg
    12061 gtatcgtgaa ggttccgcat ttgctgatga gcaccgccga agacggcaaa caggtgccat
    12121 gggtacaacc gcatgaaccg cccgtcggcg atattcattc cggttactgg gagctggcga
    12181 aagacggtat gtacggtgtt gctaaccgcc ctaacggtac ggcgcataaa tactttgcca
    12241 gcgcaccgta caaaattgcg gcgaaatctg gtaccgctca ggtcttcggt ctgaaagcga
    12301 acgaaaccta taatgcgcac aaaattgccg agcgtttacg tgaccacaaa ctgatgaccg
    12361 cctttgcgcc atacaacaat ccgcaagtgg ctgtcgccat gattctggag aacggcggcg
    12421 cgggtccggc ggttggtacg ctgatgcgcc agatcctcga ccacattatg ctgggtgata
    12481 acaataccga tctgcctgcg gaaaatcctg cggttgccgc agcggaggac cattaatcat
    12541 gacggataat ccgaataaaa aaacattctg ggataaagtc catctcgatc ccacaatgct
    12601 gctgatctta ctggcattgc tggtttacag cgccctggtt atctggagcg ccagcggtca
    12661 ggatattggc atgatggagc gtaaaatagg ccaaatcgcg atgggtctgg tcatcatggt
    12721 ggtgatggcg caaattcctc cacgcgttta tgaaggctgg gccccctatc tctatatcat
    12781 ctgtattatt ttgctggtgg cggtagatgc tttcggtgcc atctctaaag gcgctcaacg
    12841 ctggctggac ctcggtattg ttcgttttca gccgtcggaa attgccaaaa tagccgtacc
    12901 actgatggtt gcgcgcttta tcaaccgcga cgtttgcccg ccatcgttga agaacactgg
    12961 catcgcgctg gtgctgatat ttatgcccac gctgctggtg gctgcacagc ctgacctggg
    13021 aacatcaatc ctcgttgcgc tttccggtct gtttgtactg ttcctctctg gccttagctg
    13081 gcgtctgatt ggcgtcgcag tagtgctggt ggcggcgttc attcctattc tgtggttctt
    13141 cctgatgcat gattaccagc gccagcgcgt aatgatgctc ctggacccgg aatcagaccc
    13201 actcggcgcg ggctatcaca ttattcagtc taaaattgct attggctccg gcggattacg
    13261 cggcaaaggc tggctgcatg gcactcagtc acaacttgaa tttctacccg aacgccatac
    13321 cgactttatc ttcgcggtac tggcggaaga gctgggatta gtgggcattc tgattctgct
    13381 cgctctctac attctgctga tcatgcgcgg gctgtggata gccgccagag cgcaaaccac
    13441 ctttggtcgc gtcatggctg gcggcttaat gctgatatta ttcgtttatg tcttcgtaaa
    13501 tattggtatg gtaagcggta ttctgccggt tgtaggggtt ccgctcccac tggtcagtta
    13561 tggaggatca gcgctaattg tgctgatggc tgggttcggg attgtaatgt caatccacac
    13621 ccacaggaaa atgttgtcga aaagcgtgta agaggtgcgc aatgcgtaag cagtggctcg
    13681 ggatctgcat cgcggcagga atgctcgcgg catgtacaag cgatgatggt cagcaacaga
    13741 cggtaagtgt accgcagcct gcggtatgta acggccccat agttgaaatt agcggggcgg
    13801 acccgcgttt cgaaccactg aacgcgacgg caaatcagga ttaccagcgc gacggtaaaa
    13861 gctacaaaat cgtgcaggat ccgtctcgat ttagccaggc gggactggcg gcaatctatg
    13921 atgccgaacc aggcagtaac ctgacggcct ctggcgaagc tttcgatccg acacagttga
    13981 cggcggccca tccaacgctt ccgatcccca gctacgccag aatcactaac ctggctaacg
    14041 ggcgaatgat cgtggtgcgc attaatgatc gcggccccta cggcaacgac cgcgtaattt
    14101 cgctttctcg cgcagcagct gaccgtctta acacgtcaaa caacaccaaa gttcgtatcg
    14161 atccgattat tgtcgcccag gatggttcgc tttctggtcc tggtatggct tgtaccacag
    14221 tcgccaaaca gacttacgcc ctgcctgcac ctcccgattt aagcggtggc gcgggggcaa
    14281 gttcagtgtc tggcccgcag ggtgacattc ttccggtcag taactcgacg ctaaaaagcg
    14341 aagatccgac cggcgcgccg gtaaccagta gcggtttcct cggcgctcca acgaccttag
    14401 cgcctggtgt actggaaagc agcgaaccga cacctgcacc acagcccgtt gttacagctc
    14461 cgtcgacaac gcctgcaacc tcgcctgcaa tggtgacacc gcaagccgcc tcgcaaagcg
    14521 ccagcggcaa ctttatggtg caagtcgggg ccgttagcga tcaggctcgt gcgcaacagt
    14581 accaacagca actgggacag aagttcggcg tccccggtcg cgtaactcaa aatggcgcgg
    14641 tctggcgtat ccagcttggc ccattcgcca gtaaagccga agccagtacc ttgcagcaac
    14701 gtttgcaaac cgaagcccaa ttacagtcat ttattaccac cgcgcagtag cgtaaagcag
    14761 gcatctgatg tgtcaatttc tgtaaatgac gttaacaaag tcacgcggaa agtcagatgc
    14821 ctgccggtag tgcatttgct atagtagggc acttttttta attccatcac ggatgtcgta
    14881 gttcagacca tgaataccat tttttccgct cgtatcatga agcgcctggc gctcaccacg
    14941 gctctttgca cagcctttat ctctgctgca catgccgatg acctgaatat caaaactatg
    15001 atcccgggtg taccgcagat cgatgcggag tcctacatcc tgattgacta taactccggc
    15061 aaagtgctcg ccgaacagaa cgcagatgtc cgccgcgatc ctgctagcct gaccaaaatg
    15121 atgaccagtt acgttatcgg ccaggcaatg aaagccggta aatttaaaga aactgattta
    15181 gtcactatcg gcaacgacgc atgggccacc ggtaacccgg tgtttaaagg ttcttcgctg
    15241 atgttcctca aaccgggcat gcaggttccg gtttctcagc tgatccgcgg tattaacctg
    15301 caatcgggta acgatgcttg cgtcgccatg gccgattttg ccgctggtag ccaggacgct
    15361 tttgttggct tgatgaacag ctacgttaac gccctgggcc tgaaaaacac ccacttccag
    15421 acggtacatg gtctggatgc tgatggtcag tacagctccg cgcgagatat ggcgctgatc
    15481 ggccaggcat tgatccgtga cgtaccgaat gaatactcga tctataaaga aaaagaattt
    15541 acgtttaacg gtattcgcca gctgaaccgt aacggcctgt tatgggataa cagcctgaat
    15601 gtcgacggca tcaaaaccgg acacactgac aaagcaggtt acaaccttgt tgcttctgcg
    15661 actgaaggcc agatgcgctt gatttctgcg gtgatgggcg gacgtacttt taaaggccgt
    15721 gaagccgaaa gtaaaaaact gctaacctgg ggcttccgtt tcttcgaaac cgttaaccca
    15781 ctgaaagtag gtaaagagtt cgcctctgaa ccggtttggt ttggtgattc tgatcgcgct
    15841 tcgttagggg ttgataaaga cgtgtacctg accattccac gtggtcgcat gaaagatctg
    15901 aaagccagct atgtgctgaa cagcagtgaa ttgcatgcgc cgctgcaaaa gaatcaggtc
    15961 gtcggaacta tcaacttcca gcttgatggc aaaacgatcg agcaacgccc gctggttgtg
    16021 ttgcaagaaa tcccggaagg taacttcttc ggcaaaatca ttgattacat taaattaatg
    16081 ttccatcact ggtttggtta aaaattgaac acttgaaagt gtaatttccg tccccatata
    16141 ctaagcatca gtaaaaaaac tcccgccttc tggcgggagt tgctatttaa ttacgttacg
    16201 ccggagctga catgaaaacc aaacttaacg aactgcttga attccctact ccttttactt
    16261 acaaagttat ggggcaggcg ttacctgagc tggttgatca ggtggttgaa gtggtacagc
    16321 gccatgcgcc aggtgactac accccaacgg taaaaccaag cagcaaaggc aactaccact
    16381 cggtatctat cactatcaac gccactcata tcgagcaggt tgaaacactg tatgaagaac
    16441 tgggcaaaat cgatattgtc cgcatggttc tgtaactcgc ttctccgtta cccggctcct
    16501 ggtcgggtaa ctcccctctc ctcgctgtga tatacttttc ccccactttt actcattctc
    16561 cacggagatg ccgttttgta tcaggataaa attcttgtcc gccagctcgg tcttcagcct
    16621 tacgagccaa tctcccaggc tatgcatgaa ttcaccgata cccgcgatga tagtaccctt
    16681 gatgaaatct ggctggtcga gcactatccg gtattcaccc aaggtcaggc aggaaaagcg
    16741 gagcacattt taatgccggg tgatattccg gtgatccaga gcgatcgcgg tgggcaggtg
    16801 acttatcacg ggccgggaca acaggtgatg tatgtgttgc ttaacctgaa acgccgtaaa
    16861 ctcggtgtgc gtgaactggt gaccttgctt gagcaaacag tggtgaatac cctggctgaa
    16921 ctgggtatag aagcgcatcc tcgggctgac gcgcctggtg tctatgtcgg ggaaaagaaa
    16981 atttgctcac tgggtttacg aattcgacgc ggttgttcat tccacggtct ggcattaaac
    17041 gtcaatatgg atctttcacc atttttacgt attaatcctt gtgggtatgc cggaatggaa
    17101 atggctaaaa tatcacaatg gaaacccgaa gcgacgacta ataatattgc tccacgttta
    17161 ctggaaaata ttttagcgct actaaacaat ccggacttcg aatatattac cgcttaattc
    17221 catacatcaa tggcccaatt tacactgggt cattactcat aatacctgct aatgctttac
    17281 atctccttcg cgtttgtata ttttctttat cacttgcaga aatgcggtgc cacgccttgc
    17341 tccgcaactc accccattga ttaacctatt gaaatagttg attatggtga gcaaaagcgt
    17401 tccgttccaa ttagacataa gattattgta attttttaaa tattattttt
//
LOCUS       DAZGJW010000094        17396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZGJW010000094 DAZGJW010000000
VERSION     DAZGJW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.094"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(205..1795)
                     /gene="lsrK"
                     /locus_tag="ACHKIK_003227"
                     /pseudo
     CDS             complement(205..1795)
                     /gene="lsrK"
                     /locus_tag="ACHKIK_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
     gene            complement(1874..2827)
                     /gene="lsrR"
                     /locus_tag="ACHKIK_003228"
     CDS             complement(1874..2827)
                     /gene="lsrR"
                     /locus_tag="ACHKIK_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO9084348.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3076..4611
                     /gene="lsrA"
                     /locus_tag="ACHKIK_003229"
     CDS             3076..4611
                     /gene="lsrA"
                     /locus_tag="ACHKIK_003229"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO9084349.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLEALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASPKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSMTLNAGEILGLAGLVGAGRTELAETFYGLRTLRSGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIS
                     AQNVAVLLISSDLEEIELMTDRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4605..5633
                     /gene="lsrC"
                     /locus_tag="ACHKIK_003230"
     CDS             4605..5633
                     /gene="lsrC"
                     /locus_tag="ACHKIK_003230"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO9084350.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5633..6625
                     /gene="lsrD"
                     /locus_tag="ACHKIK_003231"
     CDS             5633..6625
                     /gene="lsrD"
                     /locus_tag="ACHKIK_003231"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO9084351.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6637..7659
                     /gene="lsrB"
                     /locus_tag="ACHKIK_003232"
     CDS             6637..7659
                     /gene="lsrB"
                     /locus_tag="ACHKIK_003232"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO9084352.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7686..8522
                     /gene="lsrF"
                     /locus_tag="ACHKIK_003233"
                     /pseudo
     CDS             7686..8522
                     /gene="lsrF"
                     /locus_tag="ACHKIK_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462959.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
     gene            8521..9015
                     /locus_tag="ACHKIK_003234"
                     /pseudo
     CDS             8521..9015
                     /locus_tag="ACHKIK_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(9019..9933)
                     /gene="yneE"
                     /locus_tag="ACHKIK_003235"
     CDS             complement(9019..9933)
                     /gene="yneE"
                     /locus_tag="ACHKIK_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO9084353.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDIQFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10140..11591)
                     /gene="uxaB"
                     /locus_tag="ACHKIK_003236"
     CDS             complement(10140..11591)
                     /gene="uxaB"
                     /locus_tag="ACHKIK_003236"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO9084354.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLARQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(11818..13236)
                     /locus_tag="ACHKIK_003237"
     CDS             complement(11818..13236)
                     /locus_tag="ACHKIK_003237"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO9084355.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(13375..13734)
                     /gene="yneG"
                     /locus_tag="ACHKIK_003238"
     CDS             complement(13375..13734)
                     /gene="yneG"
                     /locus_tag="ACHKIK_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO9084356.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(13734..14660)
                     /gene="glsB"
                     /locus_tag="ACHKIK_003239"
     CDS             complement(13734..14660)
                     /gene="glsB"
                     /locus_tag="ACHKIK_003239"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO9084357.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(14724..16112)
                     /gene="sad"
                     /locus_tag="ACHKIK_003240"
     CDS             complement(14724..16112)
                     /gene="sad"
                     /locus_tag="ACHKIK_003240"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO9084358.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16213..17094
                     /gene="yneJ"
                     /locus_tag="ACHKIK_003241"
     CDS             16213..17094
                     /gene="yneJ"
                     /locus_tag="ACHKIK_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9084359.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17172..>17396
                     /gene="yneK"
                     /locus_tag="ACHKIK_003242"
     CDS             17172..>17396
                     /gene="yneK"
                     /locus_tag="ACHKIK_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO9084360.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFR"
BASE COUNT         4369 a         4456 c         4318 g         4253 t
ORIGIN      
        1 gtctttagga atattcgcta taaaataagg gttatgctta atgctttaag aaaaatagca
       61 attttccctt gaatatagta ctggtgatgg aacgatgaat ctgcaagctg gcatttaaca
      121 agccagctct aaaagaaggg aaataagaaa aactatactc aaaaactaac agccacggtc
      181 atcatgatgt ggccgtcaat gaaactataa cccaggcgct ttccataacg acgtcgtcag
      241 tccatgatca accagcccca gctgatcctg ataaactgcc tgccacttat cgcgtgaatc
      301 ctgataaagt tcatgctttt ccgggtctgg tgtgtgcgtc cgttcccagc gaaccaggcg
      361 ttctccggtt tctgccattg atgaaaaaat tccggcaccg acgccagctg caatggcaca
      421 tcctaatgcg gtggcttctt tgactaccgg aatattgacg ggtaatcccg agacatcagc
      481 gagaatttga ctccataatt tcccttttga acctccgcca gcaaaaacca gcgaggtggg
      541 atgaatattc gagaaatcag caatttgctg caagttacac gctgatacaa tcgccgcatt
      601 ttcttccagc gcacggaaca atgtcgcttt gttacattta tccgggtcaa tggacaagtt
      661 aataaaggaa ggcgcagcgt gataccaggt tttaaagcgc attctgtcgg agaagatcgg
      721 cattacgccc cacgacccag gcggcacccg actggccatc tcttccagca gcgtataggt
      781 gtcgatgcct aaacgttcgg caatcagttt ttcttcggca cagaaagcat cgcggaacca
      841 gcgcatggtg agtccggtaa aaaagcttat agattcagct tgtaccatgc caggaataac
      901 atgaggatta acgcgcacgt tcatttctgg gtctgtcacc ggcgcggcta aatttacaac
      961 ttgctgccag aatgtgccgc caagaaccgc ggtttgtgcc ggacgcacaa cgcctaaccc
     1021 aaggcaacca agctgcacgt cgcctcctcc aacgaccacc ggagtgcccg ccttcagacc
     1081 gcagagttcc gccgcttgtg aacttaccac gcccagcaat gtgccggttt ctttgacagg
     1141 agaaagaata tcggcacgca ggccagccat atccagcaat gcaggtttcc agtcacgggt
     1201 ggttagatca aagtcccgtg gtgccagcgt tagagggatc caccgccagt tcgccgctga
     1261 gcatataggc cagccagtcg ctgatcatgg tgatggttga tgcctgacgg taaatatcgg
     1321 aacgatggtg cgccagccaa agtaatctgg ggatggcact taaagccagt gtttgtccgg
     1381 tcgcgcgata aacttcgttt tcaaaggtat tgttgtgcag ttctttaagt tcgctaactt
     1441 cgcgcgctgc tctggcatcc acattggcgc aggcccagat cggtgttcct tcattgttat
     1501 ataaaacaat gccttcacgc atagaacatg ccgaaacggc agcgatatac tccgggtcta
     1561 tgccggcgtt gtgcagcgcc tggcgcatac actcacacgc cagttgccag tttttgttga
     1621 gatcaaattc catagaacca ggaacgtccg gtactgccag atgccgccac tccgcctgtc
     1681 ccactgctat ttgattgcct ttcaggtcga aaatcacagc ccgaatactt ccggtgcctg
     1741 catccagcgc caacaggtaa tactttgatt ctgaaggggt aaagagtcga gccattagat
     1801 tatcctcggc ttatgcgttc tgtctgttcc tctatacgtt ctccatcatt cccggtaata
     1861 aggtcatgca aatttaacta cgtaaaatcg ccgctgctgt atcctgatcg gtaaccagtg
     1921 cgttgatata accgcctttc attgcagcgg caattgcttc ggctttattt tctccccctg
     1981 ccacgccaac ccggacgggt atggtcttca gcgcgcttaa aggtaagcca atcagttcgt
     2041 tatgtatttt aatatccgtg acaacgtcac cttttgcatc aaaaaagtag cctaaaatgt
     2101 cgccaaccgc cccttttcga ccaatcatta actgttcgcc ctggctgata taaccggagc
     2161 gaatgatcgt cgcgtcgtcc tgttgactga cggccccaat gccgacaatc gccacatccg
     2221 ctgcttgcgc ggctaacaga acatctttga cgcaattttc attttttagc gtacgggcaa
     2281 tgtcagcgga ggatgcccgc aacggagccg gaataatatt cacactgcac gccgcgttaa
     2341 gctgcccgat acccgtcata taagaaccga cgccaccgga gagcgtgacc aggcgaattt
     2401 gctgtgacga aataaaacca cttaagcgtt gcagcgtatt catggttgcc tcgccaaaac
     2461 caatcgccag catctgttgt ggttgaagta aactcatcaa catatgcgcc gcgcctatcc
     2521 ctagtcgccc gccgacatca gcatccgcaa gcccagggat cacccggaca tgttgcagcg
     2581 aaaactgacg acgtaattga gtttcatatt ccagacagcc ttcaaagcga gaattaatct
     2641 gtacgcgaat aatgccggac tgatgccctt tctccagcaa gcgcgacact ttcaaacggg
     2701 tcaggccgag acgatcgctg atctcgctct gggtcagccc gtcgtgatag taaaaccacg
     2761 cgatccgcgc gacctgttct tcttcacaca ttccctgttc tgaaattgcc gaatcgttga
     2821 ttgtcataat tcattcttca ctttgaacat atttaaatct ttaatgcaat tgttcagttc
     2881 ttgcgcattt atatctgtga tggcagccac agtttgactc tacgagcatg aacaaacgca
     2941 accgtgaaaa tcaaaatagc ataaattgtg atctattcgt cggaaatatg tgcaatgtcc
     3001 acctaaggtt atgaacaaat taaaagcaga aatacatttg ttcaaaactc acctgcaaaa
     3061 ctgaacgggg gaaatatgca aacgagtgat acccgcgcgt taccgctact ttgcgcccgc
     3121 tcggtttata aacagtattc aggggtcaat gtcctgaaag gcatcgattt tacgttgcat
     3181 cagggggagg tccacgccct gctcggcggc aatggtgccg gtaaatcgac gttaatgaag
     3241 attattgccg gtattacccc tgctgatagc ggtacgctgg agattggggg caacaactac
     3301 gccagattaa cgccagttca tgctcatcag ctgggcattt atctcgttcc ccaggaaccg
     3361 ctgcttttcc caagcctgtc gataaaagaa aacatcctgt ttgggatggc aaaaaaacag
     3421 ctctccatgc aaaaaatgaa gaacttgctg gaggcgctgg gctgccagtt tgatctgcat
     3481 agtctggcag gatcgctgga tgtcgccgat cgccaaatgg tggaaatcct ccgcgggcta
     3541 atgcgcgact cgcggattct gatcctcgat gaacctaccg cctcgcttac ccctgcggaa
     3601 accgaacgct tgtttactcg cttgcaagag ctgcttgcta ctggcgtggg tattgttttt
     3661 atctcgcata agctgccgga aattcgccag attgccgatc gaattagcgt gatgcgcgac
     3721 ggaaccatcg ccttaagcgg caaaaccagc gaactgtcta ccgacgacat tattcaggcc
     3781 atcacgccag tggtacggga aaaatcgctc tctgccagcc caaaattatg gctggagtta
     3841 cctggtaacc gcccacaaca tgctgccgga acgccggtgc tgacactgga aaatctgacc
     3901 ggcgaaggtt tcaggaatgt cagcatgacg ctcaatgccg gagaaattct gggcctggct
     3961 gggctggtgg gggccggacg cacagaactg gccgagacgt tctatggtct gcgtactttg
     4021 cgtagcggac gcattatgct gaatggtaaa gagatcaata aattatccac tggagaacgt
     4081 ttactgcgcg gtctggttta tctgccggaa gatcgccagt catccggact gaatctcgat
     4141 gcttcgctgg cctggaacgt ctgcgccctt actcataacc ttcgtggatt ctgggcgaaa
     4201 accgcgaaag ataatgccac cctggaacgt tatcgtcggg cgctgaatat taaatttaac
     4261 caaccggaac aagctgcacg gacattatcc ggtggcaacc agcaaaaaat cctcattgcc
     4321 aaatgcctgg aagcttcgcc gcaagcattg attgtcgatg agccgacgcg cggcgtggat
     4381 gtctcggccc gtaatgatat ctaccagctg ttgcgcagca tctccgcaca gaatgtggct
     4441 gtgctgctta tctcctctga tctggaagag atcgaactga tgacagatcg tgtgtatgtg
     4501 atgcatcagg gcgaaattac ccactctgca ctgaccgggc gcgatattaa tgtcgagact
     4561 attatgcgcg ttgccttcgg cgatagtcag cgtcaggagg catcatgctg aagtttattc
     4621 agaacaaccg tgaaatcacg gcattgctgg cggtggtgct gctgtttgta ttacccggtt
     4681 ttctcgaccg tcagtattta agtgtgcaaa cgctgaccat ggtttatagc agcgcgcaaa
     4741 tcctgatcct gctggcaatg ggcgcgacac tggtaatgct tacgcgcaat attgatgttt
     4801 cagtgggttc gattaccgga atgtgcgcgg tgctgttggg gatgttactg aacgcaggat
     4861 attcactacc tgttgcctgt gtcgcgactt tactgcttgg tttgctcgcg ggatttttca
     4921 acggtgtcct ggtcgcgtgg ctaaagatcc ctgccattgt tgccaccctt ggcacgttag
     4981 ggttgtacag aggcatcatg ttgctgtgga ctggcggtaa atggattgaa gggttacccg
     5041 ccgaactgaa acagctctcc gcaccgctgc tgtttggcgt ttccgcgatt ggttggttga
     5101 cgataattct ggtggcattt atggcctggc tgctggcaaa gacggcgttt ggacgcagtt
     5161 tttatgccac gggcgataat ttacagggcg ctcgtcaact gggtgttcgt acggaagcca
     5221 ttcgcattgt ggcattttcg ttgaacggct gcatggcggc actggcggga attgtgtttg
     5281 cttcgcagat tggttttatc cccaaccaga ccggtaccgg gctggagatg aaagcaattg
     5341 cagcctgcgt gctgggcggc attagtttgc tcggcggttc cggtgcgatc atcggtgcgg
     5401 tactcggcgc atggttcctg acgcagatcg atagcgtact ggtgctgttg cgcattccgg
     5461 catggtggaa cgattttatc gcgggcctcg tgctgctggc ggtgctggtg tttgatggac
     5521 gcctgcgttg tgcgctggaa cgtaatctac ggcggcaaaa atatgcccgc tttatgacgc
     5581 caccgccatc cgttaaaccc gcttcgtcag gtaaaaaacg ggaggccgca taatgcgtat
     5641 tcgctacggt tgggaactgg ctcttgccgc actgctcgtt attgagattg tcgcatttgg
     5701 tgcaattaac ccgcgaatgt tagatctcaa tatgttgctg ttcagcacca gtgactttat
     5761 ctgcattggc attgtcgccc taccgctgac gatggtgatt gtcagtggcg ggatcgatat
     5821 ttcgtttggt tcgaccatcg gcctctgcgc cattgcattg ggcgtactgt ttcaaagtgg
     5881 tgtgccgatg ccgctggcga tactcctgac cttactgctc ggcgcattgt gcgggctgat
     5941 caacgccgga ttaattatct ataccaaagt taacccgctg gtgattacgc ttggcacgct
     6001 gtatctgttt gccggaagcg ctctgctgct ttccggtatg gccggagcga cggggtacga
     6061 aggtattggt ggattcccga tggcgtttac agatttcgct aacctggatg tgctgggact
     6121 ccccgttccg ctgattatct tcctgatatg tctcctcgtt ttctggctct ggctgcataa
     6181 aacccatgcc ggacgtaatg tgtttttgat tgggcaaagc ccgcgcgtgg cgctttatag
     6241 cgcgattcca gttaaccgca ccttatgtgc gctctatgcc atgacggggc tggcgtctgc
     6301 ggtcgccgct gtgctgctgg tatcgtattt tggttcagca cgttccgatc tcggtgcgtc
     6361 gtttctgatg cccgccatca ccgccgtggt gcttggcggg gccaatattt atggtggttc
     6421 cggttccatt atcggcaccg ccattgcggt tttattagtg ggatatttgc agcaaggttt
     6481 gcaaatggca ggagtgccaa atcaggtgtc cagcgccctt tccggtgcgc tacttatcgt
     6541 cgttgtcgta ggtcgttccg ttagcctgca tcgccagcaa attaaagagt ggctggcgcg
     6601 tcgggccaat aacccattgc cataaaggat atcttcatga cacttcatcg ctttaagaaa
     6661 atcgccttac ttagcgctct tggcattgcc gcaatctcta tgaatgtgca ggccgcagag
     6721 cgtattgcat ttattcccaa actggttggc gtgggatttt ttaccagcgg tggcaacggc
     6781 gcacaacaag cgggtaaaga gctgggcgtt gatgtgacct acgacgggcc gacagaaccc
     6841 agtgtttctg gtcaggtaca gttgattaat aacttcgtca atcaaggtta taacgccatt
     6901 atcgtttctg cggtttcgcc tgatggcttg tgtccggcac tgaaacgcgc catgcaacgt
     6961 ggtgtgagag tgctgacctg ggactctgat actaaaccgg agtgccgctc ttactacatt
     7021 aatcagggaa cgcccgccca gttgggaggt atgttggtgg atatggcggc gcgtcaggtg
     7081 aataaagaca aagccaaagt cgcgtttttc tactcaagcc ccaccgttac ggaccaaaac
     7141 cagtgggtga aagaagcgaa agcgaaaatc gccaaagagc atcctggctg ggaaattgtc
     7201 actacgcagt ttggctataa cgatgccact aaatcattac aaaccgcaga aggaatatta
     7261 aaagcgtata gcgatctcga cgccattatc gcccccgacg ccaacgccct gcccgctgcc
     7321 gcacaagccg cagaaaacct gaaaaatgac aaagttgcga ttgtcggatt cagtacgcca
     7381 aacgtgatgc gtccatatgt ggaacgcggc acggtgaaag aatttggcct gtgggatgtg
     7441 gttcagcaag gcaaaatttc agtgtatgtc gcggatgcat tattgaaaaa aggatcaatg
     7501 aaaacgggcg acaagctgga tatccagggc gtaggtcagg tggaagtctc gccaaacagc
     7561 gttcagggct atgactacga agcggatggt aatggcatcg tactgttacc ggagcgcgtg
     7621 atattcaaca aagagaatat cggcaaatac gatttctgat gtgcattact taaccggagt
     7681 aagttatggc agatttagac gatattaaag atggtaaaga ttttcgtacc gatcaatcgc
     7741 aacaaaatat cccttttatc ctgaaaggtt gcggtgcgct ggattgggga atgcagtcac
     7801 gcttatcgcg gatatttaat ccgaaaacgg gtaaaaccgt gatgctggcc tttgaccatg
     7861 gttattttca ggggccgact accggacttg aacgcattga tataaatatc gccccgctgt
     7921 ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt gcgcagcgta gttcccccag
     7981 cgaccaataa gccggtggta ctgcgggcgt caggagcgaa ctctattctg gcggaattaa
     8041 gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg cctgaacagt tgcgcggtgg
     8101 cggcgcaggt ttatatcggc agcgaatatg aacatcagtc gatcaaaaat attattcagc
     8161 tggttgatgc cggaatgaaa gtgggaatgc cgaccatggc cgtgactggc gtgggcaaag
     8221 atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg aatcgccgct gaaatggggg
     8281 cgcaaattat caaaacctat tatgtcgaaa aaggttttga acggattgtt gccggatgtc
     8341 cggtacccat tgttattgct ggcggtaaaa aattaccgga gcgcgaggcg ctggaaatgt
     8401 gctggcaggc tatcgatcag ggcgcttctg gtgtggatat ggggcgtaat attttccagt
     8461 ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt ggttcaccat aacgaaacgg
     8521 caaccggaac aggcactcga tctgattttt gctaatgcct cactgcaatg gctgcccgac
     8581 cactacgaat tgtttcctca tctggtttct ttacttaatc cgcagggcgt gctggcagta
     8641 cagatgccag ataactggct ggagccgacc catgtgctca tgcgcgaagt tgcctgggaa
     8701 caaaactacc cggatcgcgg gcgtgagccg ttggctggcg ttcatgctta ctacgatatt
     8761 ttgagcgaag ccggatgtga ggtcgatatc tggcgaacca cttactatca ccagatgccg
     8821 tcgcaccagg cgattatcga ttgggtgacc gccactggat tacgtccgtg gttacaggat
     8881 ctgaccgaga gcgaacagca gctttttctt acgcgctacc atcagatgct ggaagagcag
     8941 tatccactgc aagagaacgg acagatactg ctggcatttc cgcgtctgtt tattgttgcc
     9001 cgccgtacgg agtaattatc acgtcagctg gtaatgacga tcgggaagaa tttttgctgg
     9061 aatttctgct tcatcgttca tctgtaacag gtcaatttca atagcgttgc agatggcatc
     9121 cagcggtaaa tcattgtttt cagtaccgaa cggatcttcc agttcttccg ccagacaatc
     9181 cagcgaaata aaagtgtagg aaatcagcac agagataaaa ggcgtcatgt aatgcaggtc
     9241 cacgaccagc gcgaacggca gcatgataca aaacagataa acggtacgat gcaaaatcag
     9301 ggtgtaggca aagggaattg gcgtgtaggc aatgcgctca cagcctgcca gaaccgctga
     9361 aatatcatta agccgatcgt tgaggctaat aaactggata tctgaaagct gcccattgcg
     9421 gcgctgaacc gccaaccact cccccattat taacaagata cggttagctg gagagttcga
     9481 agccagtaca ggctgaagat cttcagtctt gagataatga gccagcactt ccgcctgtgg
     9541 ctgtttgcgt aatgtcatgc gtaaacagtg ggcgaaggcg atttgcaacc gggcaaactc
     9601 ccttacactt gccgaatccg gcaatgtcgt ttttacctcg cgcagtaacg accgtgaggc
     9661 aatcatcaac tgcccccaaa gttttcgcgc ttcaacgtaa cgggcgtacc cggcattatt
     9721 acgaaaacca agaaaaatgg cgatggcgac accgagaatg ctgaacggtg cgagggtgaa
     9781 tttgatgccc agatgcgtgt accagggcag catgaaaata acagcgatag aaaagagaaa
     9841 attgagtagt aagcgcgatg atatcttgga taatactgag ccgtgccaga caaaaatacg
     9901 gcgcagccag tgttgttgtg gacgaacaat catggttatc ttcaggcgtg aaaaagtcgc
     9961 cctattaaac gtgattacga tcacattctc aagacgctac ttacaaatta cctacaagct
    10021 tacaacatta acaggcgctg catgtggcat ctgttaatga taaaggcaca taggatgttg
    10081 taactaatat agtgaaataa gaaacccggt cgaaaccggg ttcagaagta acggtgttat
    10141 tagcacaacg gacgtacagc ttcgcgcatc cctttttcaa gaatcgcatc caggtcatta
    10201 gcaacctgct cgaccagacc aggcacttgc gtcaggtcct gctcccagtg gtctttttct
    10261 gccaacacaa tcgctaccag ttcctgggta ccgataacac ggtcacgatg ctggctccag
    10321 agttgctgat aacgttccag ccagtgtgca tcgtcctgta ccggatatgt ttctccgtta
    10381 cgctcaccac gatagaaggc aatcaatgcc gctaatgcga aagtaaggcg cgccggaagt
    10441 gtgccttttg ccttctgccg tgccagcagc tgcggcagga tgcgagtgcg gaacttggtc
    10501 ataccgttga gcgcgataga cagcagctga tgcttaatgt acgggttacg gaaacgcccg
    10561 gtgactgcac tggcgaaaga ttccagttca tcacgaggca aatccagtac cggaataatt
    10621 tcttcgtaga tagctttttc aacgaatgcg caaatttcag catcgttcat cgcttcacct
    10681 acggtatcca gccctgcctg gaacgccacc ggcaccagcg cggtgtgcgc accgttgagg
    10741 attgccactt tgcgttcttt gtacggttta atatcgtcaa caatcagcac gttgagcgga
    10801 tatttgtcca gacgcagttc agtcgctaag gatttcggtc cctgaatcac aaacaggtaa
    10861 aagtgttcag cggtgtcgag aaaaccatcg tgataaccca gctcttcttc cagtttagcc
    10921 acttcatcgc gcggataacc ggtaacgata cggtccacca gcgtagaaca gaagctgtta
    10981 gcctgatcca gccattgaat aaatgcttct ggcagtgccc actcttgcgc atagcgcagc
    11041 accagttcac gcaacgcgtc gccattgtag tcgatcaact cacacgggat gatgatccaa
    11101 cctttatcca gcgcaccgtt gaaatggcta aaacgttcga acagcagacg ggtcagtttt
    11161 gccggataac ttaccgctgg cgcgtcatcg aatttatcgc ccgcatggta gctgataccc
    11221 gcttcggtgg tattagagaa aacaaagcgc atttccgggt tgtgagccag tttcaggaat
    11281 tcatcgtatt cactgtagac gctgatttca cgattaaccg agcgaatcag acgcgcgtcg
    11341 ctgaccgctt cccccttctc attcaggcca cggataatgg tggtatacag accatcctgc
    11401 gtgctcagtg acggtgggaa tgaagtttca atcggacgaa caacgaccac accagaattc
    11461 agatcggtgt gctcattcag gagatcgatt tgccagtcaa caaaggcgcg caggaagtta
    11521 ccttcaccaa actgaatgat acgttctgga tactgtgcac cgggaaaatc gcgacgattt
    11581 agtgttttca caatgggttc ccttctgatt agtcatacaa cctgtttgaa ttggtacgac
    11641 aggttagcaa actttaatac gccgaacccc tgttttgatc aactcctgat gattaatgag
    11701 cagttttatg agaaaagtgt ggcgcggatc atggtttaat cgaggaaata cgccttttcc
    11761 tggatcataa agtggtagaa cacattgcat tcaaatcgcg cggaatgaat aaagatgtca
    11821 gacaacttcc tcaccgtaac gcatagtgct ggtacggttg cgcccatctt tcttcgagcg
    11881 atacagacac gtatcggctt cgaccatcaa tttattaaaa tcatcggtca gcgttcggta
    11941 cgatgcgcga ccactaccga cgccaatact taccgtgaga taaagcgttt tttgttgcca
    12001 ggtgaatggt tgcagttcaa cgcctttacg gattttttcc gccattagca gaccatcgac
    12061 aggattcacc gacggcactg caacagcaaa ttcttcgccg cccatgcgcg ccaccagccc
    12121 cttatcaccg acaatcttct gaatatgccg ggcaaacacg cttaacactt tatcgccaca
    12181 ttcatgtcca tagttatcgt taatgctttt gaaatagtcg atatcaagca gcatgaccgt
    12241 cagatgttgt gtctgtttca gcgatggact tttcaacgcc tcataaagac cggaccgtga
    12301 gtaaacctga gtcagaaaat caaagtcggc tcgcagcgca acttgcttca ttagcgaatt
    12361 gatcgctgcc acgctaaaag aaaccataat tgggcatatc gccatcgtgg caataccgag
    12421 acgtgcggag aacatttccg gaatggagaa cggcgaaccg accgagatat caatcaccga
    12481 atttgccacc agtacgattt ccaccgcacc ggtgacaaag gtcaacagac atgttacctg
    12541 cggcgtatag cgcactgcac accagattaa tgcaggcagc gggaacgcca gactccccgc
    12601 tccgccaatg actaccgagg caataaccga cacaataagc gcgatggcgg gcatcatctg
    12661 ttctgcttta aagcgcggca gcactccagg aatagccaac gtcagcatac aaggcacgat
    12721 caacacgccc gttgagaatt gctcactgaa ccagtccgca agcaaaggcc agaaatccag
    12781 actatcaata ctgaccgaac caatcgcccc gacaatagcg cataataatg cggcaatcag
    12841 acagtaatta aatagccgta aggcacttac tggctcatac ttatttttcc ccagacgctt
    12901 atcccgcacg accagtaagg cgacggtaac aataaacatc atattggaga aattgataac
    12961 cagtgaaacc agtccccatt cggtggttat ggcatcataa accagcatcg ccacataact
    13021 gatcgcatag taatgcagtc gattaagcca gacatagcgg gcaaacaccc ccgccattac
    13081 gccattcagc ggccagaaaa gagaaagttc gtgaaccagc cgtaactctg caccaataaa
    13141 atagaacagc gtggttagcg caaaaatagc gatgctatta cgtagcagat gaccttcctg
    13201 gaacaaacgg aaagttgaaa tggggtgtac atgcattaat tatctgtcca tacaattgcg
    13261 ggtggcaatt atctcaacgt aataagtaaa ttctcataaa ggatagcatg ttcgctaaca
    13321 gcggcggcaa ataaaggtac cctgggaatg ctattttatc gttttccgca attctcatgg
    13381 ctgattcatc tgtacaacca gccagtgata gattaccgcc acgatataac gttgttgttc
    13441 ttcgcttaac gaaacaccct gcggaatgtt atgccactta gccagacaac cgcgacagca
    13501 ggtcgcggtg gcgtgctgag cgataaacac cggatgcccg cgcatggggg tttgcttacc
    13561 gtcattagcc ggtaatgccg gagcaagacg tttagcgaca aaatccgccg catgttgttc
    13621 gatgactggc gcgcctttct ccaggcaata ctgacgctct ttcatgccca gacgaaagcg
    13681 agagcgaaat tttgaacggg ataaacgcgc gaagagtgga tcgagagact gcattaataa
    13741 accgaacgcc ctaactgttt cgtcaattgt tcaaggacgg caatacccgc aagcgagtta
    13801 cctgcatcat ccagttccgg actccagaca gcgatggcca tttcatgcgg aacaatcgcc
    13861 acaataccgc caccaacgcc agatttcgcc ggtagcccca cccgccaggc aaactccccc
    13921 gcgttctggt acataccact ggtcgccatc agcgcgttaa tttgccgcgc ctgcattggc
    13981 gtcaccacag gttcatcaat atgaatagct ttcccctgat tagccagaaa gacaaacgtc
    14041 ctggccagct ctacacagct cattttcaga gcgcagtaat gaaagtagtt ttgcagaacg
    14101 gttgtcacat catgatggaa attgccaaac gacttcatca gccaggcgat agccgcattt
    14161 cgcgcggaat gttcaaattc ggaacgcgct accaccgtat cgtatgaaat atcagacaca
    14221 ccgcttaagc cgcgcacgac ttccagcata cgttgccgtg gtgcgcttaa tcgcccttgc
    14281 aacatatcgc aaaccaccag cgcaccagca ttaataaacg gattacgcgg aataccctgc
    14341 tccatttcca gttgcactaa agaattgaac ggtgatccag acggatcttt gccgacgcgt
    14401 tgccagattt cctcttcgga gtaatgacgc atggcgacaa cgagactcag cactttggaa
    14461 atagactgaa tggaaaaacg ttcttgcgcg tctccggcct gaaaaagctg tccgtcaacg
    14521 gtacagatag caatgcccaa tcgggaaccg tctactgtag ccagcgccgg aatataatcc
    14581 gcgactttac cctgaccaat gagcggccgc acttgccgca agatgttttc taaaattgca
    14641 ttatccatgg cgactgccac tttctactcc tggaccgcag gtctgaaaag acctgcgagt
    14701 atatcagagc tgaatatgtc gcgtcagatc cggtctttcc acaccgtctg gatattacag
    14761 aattcgtgta agccgaaatg ggaaagctca cgaccaaagc cactcttttt cacgccacca
    14821 aaggccactc gcgcgtcgct ggcacaataa ccattgataa acaccccacc gcattccaga
    14881 cgtgccgcca tctgtctggc ctgtgtttcg tcggtggtaa aaatggtcgc tgaaaggccg
    14941 aactcactat cattagccag ttccagcgca tgttctgcat ctttcgcaac ggtgattgcc
    15001 gcaacagggc caaacatttc ttcccgaaac gcggtcattt ctggggtaac attcgccaga
    15061 accgttggcg gatagtagtt acctgcccca gccatctttt ccccgcccag taacaaacgc
    15121 gcaccctgcg ccagggtttt ctccacctga tgatgcagct catcacgtaa atcaaaacga
    15181 gccattggtc cgagagcatt ctcttcatca cggggatcgc ccattttcaa ggctgccgca
    15241 gctgccacaa aacgttcggt aaatgcagaa gcaattccct cttcgataat aaagcgtttt
    15301 gccgctgcac aaacctgtcc ggtattctga taacgtccgg ctaccgccgc tttaaccgcc
    15361 agttccagat cggcatcgtt aagcacaata aatggatccg aaccgcccag ttccagtacg
    15421 cattttttca gtgccgctcc agcctgtgcg ccaatagccg ctcccgcacg aacacttccg
    15481 gtcaccgtga cagcagcaat gcgcgaatct ttaatcattt gactgacacc gtcgttgtcg
    15541 gcattcagcc agccatatac gccttgcggg attcccgcat ctttaaacac ctgggcaatg
    15601 agctgagcac agcccatcac attcggcgca tgtttaagta agtagccgtt acctgcaaga
    15661 atgatgggaa ccgcgccacg catcacctgc cataacggaa agttccacgg cataatcgcc
    15721 agaatcgtcc ccaacggtcg atactcaata actgcctgct gattttccac cagcgtaggt
    15781 tccgccttca gcattgccgg accatgttct gcataccagt cacacaaatt cgccgatttc
    15841 gccacttcag cgcgcgcctg attgattggt ttgcccattt cacgggtgat catttgcgcc
    15901 atttcttcgc tacgggcgcg cagagcctta ccgataccac gcagtttttc agcacgataa
    15961 tctatatttg tctcgcgcca gtcgcgaaag cctgctgccg ccagctgaag tgcgttttcg
    16021 atatcgttag cgccagccca cggcagcaca gaaagttgtt cacccgtggc aggatttatt
    16081 gaaattgcat gagttgccgg agtaatggtc atcggggtat ctcctttatg agtcatggta
    16141 tgaagatacg cagatttact cttgctttaa aatgaataat attaagccac ttattcacga
    16201 atcgagaatg ttatggatct gacccaactg gagatgttca acgcggttgc cgaagctggc
    16261 agcattaccc aggctgcagc aaaagtgcat cgcgtaccgt cgaatctcac tacccgtcta
    16321 cgccagctgg aaacagaact gggggtagat ctgtttattc gcgagaatca gcgtttacgt
    16381 ctctctcctg ccgggcataa ctttttacgc tatagccaac aaattctcgc gttagtggat
    16441 gaagcgcgga gcgttgtcgc tggcgatgag ccgcaaggtt tattttctct tggttcgctg
    16501 gaaagcaccg cagcagtgcg cattccggcc acgctggcgg aattcaacca tcgttatccc
    16561 aaaattcagt tttcactttc caccggccct tccggcacta tgctggaagg agtgctggag
    16621 ggaaaactga atgcggcatt tattgatggg cccattaacc atactgccat tgacgggata
    16681 ccggtatacc gcgaggagct gatgatcgtc acgccacaag gtcatgcgcc agtaatccgc
    16741 gccagtcagg ttaacggcag taacatttat gccttccgcg ccaattgttc gtatcgtcgc
    16801 cacttcgaga gctggtttca tgctgacggt gccgctccgg gaactattca cgagatggag
    16861 tcgtatcacg ggatgttggc ctgtgtggtc gcaggagcag gcattgcgct tattccacgc
    16921 tctatgctgg aaagtatgcc ggggcatcac caggttgaag cgtggccgtt agctgagcaa
    16981 tggcgttggt taacaacctg gctggtctgg cgtcgcggtg cgaaaacgcg tccgcttgag
    17041 gcatttattc aactactgga tgtgcctgac tcggcaaaac agggttacca atgagctatt
    17101 tttgatagtt cttgcagctc aaatgcccta tatagtgatg ttccgatgac ttatgactat
    17161 atggggcaaa tatggttacg ccagtaagca tcagtaatta catatctctt cctgatgatt
    17221 ttcccgtacg taatattgca cctcaggtaa aagaagtttt aaaggacttt attgatgcgc
    17281 taagtacaat aatatgtgat gaagagtggc gcacgtcttt aaacatcaac tcagccacaa
    17341 aaaagatatt taataacctt gataacttat catatattca gaggacttct tttcgg
//
LOCUS       DAZGJW010000095        17208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZGJW010000095 DAZGJW010000000
VERSION     DAZGJW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.095"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            9..593
                     /locus_tag="ACHKIK_003243"
     CDS             9..593
                     /locus_tag="ACHKIK_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9084361.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            673..1368
                     /locus_tag="ACHKIK_003244"
     CDS             673..1368
                     /locus_tag="ACHKIK_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9084362.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1398..3914
                     /locus_tag="ACHKIK_003245"
     CDS             1398..3914
                     /locus_tag="ACHKIK_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO9084363.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNRHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQNLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3925..5016
                     /locus_tag="ACHKIK_003246"
     CDS             3925..5016
                     /locus_tag="ACHKIK_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9084364.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5059..5919)
                     /gene="rsmI"
                     /locus_tag="ACHKIK_003247"
     CDS             complement(5059..5919)
                     /gene="rsmI"
                     /locus_tag="ACHKIK_003247"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO9084365.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5983..8019
                     /gene="lpoA"
                     /locus_tag="ACHKIK_003248"
     CDS             5983..8019
                     /gene="lpoA"
                     /locus_tag="ACHKIK_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO9084366.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7977..8372
                     /locus_tag="ACHKIK_003249"
     CDS             7977..8372
                     /locus_tag="ACHKIK_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO9084367.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8392..8982
                     /gene="diaA"
                     /locus_tag="ACHKIK_003250"
     CDS             8392..8982
                     /gene="diaA"
                     /locus_tag="ACHKIK_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO9084368.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            8992..9567
                     /gene="dolP"
                     /locus_tag="ACHKIK_003251"
     CDS             8992..9567
                     /gene="dolP"
                     /locus_tag="ACHKIK_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO9084369.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9681..10721)
                     /locus_tag="ACHKIK_003252"
     CDS             complement(9681..10721)
                     /locus_tag="ACHKIK_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO9084370.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMLYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10794..11429)
                     /locus_tag="ACHKIK_003253"
     CDS             complement(10794..11429)
                     /locus_tag="ACHKIK_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO9084371.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            11557..12075
                     /gene="yhbO"
                     /locus_tag="ACHKIK_003254"
     CDS             11557..12075
                     /gene="yhbO"
                     /locus_tag="ACHKIK_003254"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO9084372.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12055..12498)
                     /locus_tag="ACHKIK_003255"
     CDS             complement(12055..12498)
                     /locus_tag="ACHKIK_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO9084373.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12549..12851
                     /gene="yhbQ"
                     /locus_tag="ACHKIK_003256"
     CDS             12549..12851
                     /gene="yhbQ"
                     /locus_tag="ACHKIK_003256"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO9084374.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12838..13341)
                     /locus_tag="ACHKIK_003257"
     CDS             complement(12838..13341)
                     /locus_tag="ACHKIK_003257"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9084375.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13335..13859)
                     /locus_tag="ACHKIK_003258"
     CDS             complement(13335..13859)
                     /locus_tag="ACHKIK_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO9084376.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14068..15063
                     /locus_tag="ACHKIK_003259"
     CDS             14068..15063
                     /locus_tag="ACHKIK_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO9084377.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15072..15950
                     /locus_tag="ACHKIK_003260"
     CDS             15072..15950
                     /locus_tag="ACHKIK_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO9084378.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16156..17163
                     /locus_tag="ACHKIK_003261"
     CDS             16156..17163
                     /locus_tag="ACHKIK_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO9084379.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
BASE COUNT         4336 a         4561 c         4354 g         3957 t
ORIGIN      
        1 ggttataaat gaataaagtt acaaaaacag ctattgtggg cttacttgcg cttttcgcag
       61 gtaatgccgc tgcaaccgat ggtgaaatag tttttgatgg cgagatttta aaatccgctt
      121 gtgaaatcaa tgactctgat aagaaaattg aagttgctct tggtcactat aatgccgaac
      181 agtttcgttc tgttggcgat cgtagcccaa aaattccatt cactattcct ttagttaact
      241 gcccagtgac tgggtgggag cacgataacg gcaacgttga agcgtctttc cgtttatggc
      301 tggaaacgcg ggataatggc accgtcccta acttccctaa tctggcaaaa gtgggctctt
      361 ttgctggcac agcggcaaca ggtgtgggta tccgtattga cgatgcggaa agcggaaacc
      421 ttatgccact gaatgctatg gggaatgata acacggttta tcagatccca gcggactcca
      481 atggtattgt caatgttgac ctcatcgctt actacgtatc aaccgttgaa gcatcagaaa
      541 tcaccccagg ggaagcagac gctgtcgtta acgtaacgct ggattaccgt taatacgtta
      601 cggcgttatc tgacctttca gataacgccc ttttccttcc tctttctcgt tgtatcaggt
      661 tgaaaaataa ctatgtcaaa acgaacattc gcggtgataa taaccttgtt gtgtagcttc
      721 tgtatcggcc aggcgcttgc agggggtatc gttttacagc gaacgcgagt gatctatgat
      781 gccagccgca aagaggctgc gttacctgtc gcaaacaaag gcgcagaaac gccttattta
      841 ctgcaatcat gggtagataa tatagatggt acaagccgtg ccccatttat tataacccca
      901 ccgctatttc gtcttgaagc cggcgatgac tcatcactgc gaattattaa aacagctgat
      961 aacctgcctg aaaataaaga gtcgctgttc tacattaatg ttcgtgccat tccagcaaag
     1021 aaaaaatcag atgatgttaa tgctaacgag ttgacgctgg tatttaaaac acggatcaaa
     1081 atgttttatc gccccgcaca cctgaaggga cgggtaaacg atgcgtggac atcactggaa
     1141 tttaaacgta gtgaccattc actcaatata tataacccaa ctgaatatta cgtcgtattt
     1201 gccggactgg cagtcgataa aaccgatctc acaagcaaaa ttgaatatat cgcgcccgga
     1261 gaacataaac agttaccact tcctgcatct ggcggaaaga acgtgaaatg ggctgcaatc
     1321 aatgattatg gcggcagttc cgggacagaa actcgtccac tgcaataaca aatataaaaa
     1381 acacaggtca tcagggaatg ccacaacgac accaccaggg acataaacgc acaccgaaac
     1441 agttggcgct catcatcaaa cgctgtttgc cgatggtgct cactggcagc ggcatgcttt
     1501 gcactaccgc taacgccgaa gagtattatt tcgaccccat tatgctggaa actacaaaaa
     1561 gtggtatgca aacaactgat ctgtcacgtt tttcaaaaaa atacgcacaa ctaccaggaa
     1621 cttatcaggt tgatatctgg ctgaataaaa agaaggtttc acagaaaaaa attacattta
     1681 ccgccaatgc agagcaactt ctgcagccac agtttacggt agaacaacta cgtgagctgg
     1741 gtattaaggt ggatgaaatc ccggcgctgg ctgaaaaaga tgacgatagc gtgatcaact
     1801 cgcttgaaca aatcattccc ggtacagctg ctgaatttga tttcaatcat cagcgactta
     1861 atttgagcat tccccaaatt gcactgtacc gtgatgcaag aggttacgtc tccccttctc
     1921 gttgggacga tggtatacca acgctgttta ccaactactc gtttacaggt tctgataacc
     1981 gttaccgcca gggcaatcgt agccaacgac agtacctgaa tatgcaaaat ggtgctaatt
     2041 ttggcccctg gcgattacgt aactattcta cgtggacacg caacgatcag gcgtcaagct
     2101 ggaacactat cagtagttat ttacaacgtg atatcaaggc gttgaagtct cagttgcttc
     2161 tgggagaaag cgccaccagc ggcagtattt tttccagcta cacctttact ggcgtgcaac
     2221 tcgcttccga cgataatatg ttgccaaaca gccagcgcgg atttgcccca acggtacgcg
     2281 gtatcgcaaa cagtagtgca atcgtgacta tcaggcaaaa tggttatgtg atctatcaaa
     2341 gcaacgtgcc agcgggtgcc tttgaaatta acgatctcta cccctcttcc aacagcggcg
     2401 atttagaagt cacgattgaa gaaagtgacg gtacgcaacg tcgctttatc cagccttatt
     2461 cttcattacc catgatgcag cgacctgggc atctaaaata tagcgcgacc gctggacgct
     2521 atcgcgctga tgcaaacagt gatagcaagg aacccgaatt tgctgaagcc acggcaatat
     2581 atggtttgaa taatactttt acgctgtatg gcggcctgct cggttctgaa gattattatg
     2641 cgctggggat cggtatcggc ggcacacttg gcgcactggg cgcgttgtcg atggatatca
     2701 acagagctga cacccaattc gataaccggc actcttttca tggctatcaa tggcgtacgc
     2761 agtacatcaa agatatcccg gaaaccaaca ccaatatcgc tgttagctac tatcgctata
     2821 ccaacgatgg ctattttagt tttgatgaag ccaatacccg taattgggac tataacagtc
     2881 gccaaaaaag tgaaattcaa ttcaacatca gccagacaat atttgatggg gtaagtctgt
     2941 atgcctccgg ttcgcagcaa gactattggg gcaataacga taaaaacagg aatatctcag
     3001 ttggggtttc cggtcagcaa tggggaattg gttacagcct gaattatcaa tacagtcgct
     3061 acacggatca aaataatgac cgcgcactct ctttgaatct cagtattccg ttagaacgct
     3121 ggttaccgcg tagccgggtt tcctatcaga tgaccagcca gaaagatcgc ccaacccaac
     3181 atgaaatgcg tcttgatggc tcactgctgg atgatggtcg cctgagctat agcctggaac
     3241 aaaatctgga tgacgataac aaccataaca gtagcctgaa cgccagttac cgttcacctt
     3301 atggcacctt cagtgccgga tacagctacg gtaatgacag cagccaatac aattacggcg
     3361 ttaccggcgg cgtggttatc catcctcatg gcgtgacgct ctcgcaatat ctgggcaacg
     3421 cttttgcgct tatcgatgct aatggggctt ctggcgtgag gatacaaaac tatccgggga
     3481 ttgctactga tccctttggc tatgcagtgg ttccttatct cacgacttac caggaaaacc
     3541 gtctctcggt agatactacg cagctgcccg ataacgtcga tcttgagcaa acaacacagt
     3601 ttgtggtgcc caacagaggt gcaatggtag cggcgcgttt caacgccaat atcggttatc
     3661 gcgtacttgt tacagtcagc gatcgcaacg gtaaaccgtt gccctttggc gctcttgcca
     3721 gcaacgatga gacggggcaa caaagtatcg tcgatgaggg cggcatacta tatctctctg
     3781 ggatatcgag taaatcacaa agctggactg tacgctgggg aaatcaggca gatcaacaat
     3841 gtcagtttgc ttttagtaca ccggattcag aaccaacaac ctctgtatta caaggcacgg
     3901 cgcagtgcca ttaaggataa aaaaatgaaa agagcgcctc ttataacagg acttttgttg
     3961 atatccacat cctgcgctta tgcctcctca ggagggtgtg gagctgacag cactagcggt
     4021 gcgacaaatt acagcagtgt ggttgatgat gttacggtga accagacaga taacgtgaca
     4081 ggacgggagt ttacctctgc cacgctaagt agcactaact ggcaatacgc ctgtacctgc
     4141 tctgcgggta aggcagttaa acttgtctat atggtcagcc ctgtacttac caccactgga
     4201 catcaagcag gatattacaa actcaatgat agtctggata ttaaaaccac attacaggcg
     4261 aatgacattc caggactcgt gactgaccag accgtttctg ttaacacccg attcacacag
     4321 ataaaaagca acaccgtata ttctgctgca acccaaacgg gtgtttgcca gggtgacacg
     4381 tctcgttatg gacccgttaa tattggtgca aataccacct ttaccctgta tgtcaccaag
     4441 ccatttctcg gctcgatgac cattccgaaa acggatattg ccgtcattaa aggcgcatgg
     4501 gtcgatggaa tgggaagccc gtctacaggt gacttccatg atttagtcaa gttatcgatt
     4561 cagggaaatc tcaccgcccc acagtcgtgc aaaattaatc agggcgatgt tattaaggtt
     4621 aattttggat tcatcaatgg tcagaagttt accacccgca atgccatgcc agacggtttt
     4681 actccagtag actttgatat cacttatgac tgtggtgata cttcaaagat taaaaactcg
     4741 ttgcaaatgc gcatcgacgg tacaactggg gtagtagacc agtacaacct ggtcgccaga
     4801 cgaagaagtt cagacaatgc gcccgatgtc ggtattcgta ttgaaaatct cggcggcgga
     4861 gttgcaaata ttccttttca gaacggtatc cttcccgttg atccttccgg gcatggcacc
     4921 atcaacatgc gcgcctggcc agttaatctg gtcggtggtg agctggaaac aggaaaattt
     4981 cagggcaccg ccaccattac cgtcatcgtg cggtaaaaga agacgttgcg ttttgattaa
     5041 cgcaacgctt cggcactctt acccctgctg ctccagcgca tacttataca gcgcattttt
     5101 cttcacgccg tgaatttctg ccgccagcgc cgccgctttt ttcaacggca gttctgcctg
     5161 tagcagcgcc agcgtgcgca gggcatcggc gggtaagtct tcttcctgtg ctttatgacc
     5221 ttcgataatc agcaccattt cgcctttgcg acggttttca tcttctttta cccacgccag
     5281 cagctcgcca acgggcgcgc cgtgaatggt ttcccaggtt ttggtcagct cacgcgccag
     5341 aaccacgtag cgggattcgc ctaataccgc aacgatatct tccaggctat ctaacagacg
     5401 gtgggtggat tcataaaaaa tcagcgtgcg cggctccgct tcaatggctt ttagcgcatc
     5461 acggcggcct tttgatttgg caggtaaaaa gccttcgtaa cagaaacggt cagagggtaa
     5521 acccgcggcg cttaacgcag tgatagcagc acacggcccg ggtagcggca ccacgcaaat
     5581 ccccgcttca cggcaggtac gcaccagatg gtagccagga tcgttaatta gcggcgttcc
     5641 ggcatcggaa accagcgcaa tgttctgccc ttcttgcagc ttcgccagca gcgtttcggc
     5701 tttttgttgt tcgttatggt cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg
     5761 cagcaataaa ccggtgtgac gagtatcctc ggcggcaatc agatcaacgg cctgtaatac
     5821 ctctaacgca cgctgggtga tatccgccag attgccgatt ggcgtcggta caatgtaaag
     5881 ctggccctga gaattatccg ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat
     5941 ttaatattga gcattgcgta aaaaaatatc actggataca ttatggtacc ctcaacattt
     6001 tctcgtttga aagccgcgcg ttgtctgcct gttgttctgg cagccctgat tttcgccggt
     6061 tgtggcaccc atactcccga tcagtccact gcttatatgc agggcacggc gcaggctgat
     6121 tctgcctttt atcttcagca gatgcagcaa agctctgatg ataccaggat caactggcaa
     6181 ttactcgcca ttcgtgcact ggtgaaagaa ggtaaaaccg ggcaggcggt tgagttgttt
     6241 aaccaactac cgcaagaact gaacgattct cagcgtcgcg agaaaacact gctggcggta
     6301 gagattaaac tggcgcagaa agattttgct ggcgcgcaaa acttgctggc gaaaatcaca
     6361 cctgccgatt tagaacaaaa ccagcaagcg cgttactggc aggcaaaaat cgatgccagc
     6421 caggggcgtc cttccattga tttactgcgc gcgttaattg ctcaggaacc gctgctcggc
     6481 gcgaaagaaa aacagcagaa tattgatgcc acctggcagg cgctctcctc catgactcag
     6541 gaacaggcga atacgctggt gatcaacgcc gacgaaaata ttctgcaagg ctggctggat
     6601 ctgcagcgcg tctggtttga taaccgtaac gatcccgaca tgatgaaagc cgggatcgcc
     6661 gactggcaaa aacgttatcc gaacaatccg ggcgcgaaaa tgctgccaac gcagttggtt
     6721 aacgtaaaag cgtttaaacc agcctcgacc aacaaaatcg ccctgctgtt gccgctgaat
     6781 ggccaggcag cggtatttgg tcgcactatt cagcaaggct ttgaagcggc gaaaaatatc
     6841 ggcactcagc cagtggcggc tcaggtagct gccgcacctg ccgcagacgt agctgaacaa
     6901 cctcagccgc aaaccgtgga tggcgttgcc agcccggcac aagcctcggt tagcgatctg
     6961 accggtgaac agcctgcggc acagccagcg cctgtaagca ccccggcagc aaccaccaca
     7021 gcggtaagcg cgccagcgaa tccatccgca gaactgaaaa tctacgatac ctcatcacaa
     7081 ccacttagcc agatcttaag ccaggttcag caggatggcg cgagtattgt ggttggtccg
     7141 ttgctgaaaa ataacgttga agagttgctt aagagcaaca ccccgctgaa cgtgctggca
     7201 ctgaaccagc cggagaatat cgaaaaccgc gtcaatattt gttacttcgc gctttcaccg
     7261 gaagacgaag cgcgcgatgc agcgcgtcat attcgtgacc agggtaaaca agcgccgctg
     7321 gtgctgatcc cacgcagttc attgggcgat cgcgtagcca atgcgtttgc gcaggagtgg
     7381 cagaaactgg gcggcggcac cgttctgcaa caaaaatttg gttccaccag cgaattacgc
     7441 gcgggtgtta acggcggttc tggtatcgct ttaacgggta ccccgattac tcccagagcg
     7501 acaaccgact ccggcatgac gaccaacaat ccaacgctgc aaaccacgcc aaccgatgac
     7561 cagttcacca ataatggcgg tcgtgtcgat gcggtgtaca ttgtggcaac gccgggtgaa
     7621 atcgctttta tcaaaccgat gatcgccatg cgtaacggta gccagagcgg tgcaacgctg
     7681 tacgccagct cccgcagtgc gcaagggacc gctggcccgg atttccgact ggagatggaa
     7741 ggcttgcagt acagcgaaat cccgatgctg gcaggcggta atctaccgtt aatgcagcag
     7801 gcactcagcg cggtgaataa cgattattca ctggctcgca tgtatgcgat gggcgtcgat
     7861 gcctggtcgc tggcaaatca tttctcacaa atgcgccagg ttcagggttt tgaaatcaac
     7921 ggtaataccg gaagcctgac ggcaaacccg gattgcgtga ttaacaggaa gttatcatgg
     7981 ctacagtacc aacaaggtca ggtagtcccc gccagttaac caccaaacag accggcgatg
     8041 cgtgggaagt acaagcgcgt cgctggctgg aaggcaaagg actgcgattt gtcgccgcta
     8101 acgtgaacga gcgtggcggc gagatcgatc tgataatgcg tgaaggccgg accaccgttt
     8161 ttgtcgaggt acgctatcgc cgctctgcgc tttatggcgg cgcggcagcc agtgtgaccc
     8221 gcagcaaaca acacaaatta ttacagactg cccgcttgtg gctcgcgcgt cataatggga
     8281 gttttgatac tgtggattgc cggttcgatg tggtagcctt caccgggaat gaggttgagt
     8341 ggattaagga tgcctttaat gaccactcat aattaaggtt taaggattag cgtgcaagaa
     8401 agaattaaag cttgcttcac tgaaagcatt caaactcaaa ttgcggcggc agaggcgctt
     8461 ccggatgcca tctcccgtgc agccatgacg ctggttcagt ctctgctcaa tggcaacaaa
     8521 atcctctgtt gtggtaatgg aacttccgct gccaatgcac agcattttgc tgccagcatg
     8581 atcaaccgtt tcgaaacgga gcggcccagc ttacctgcca ttgcactaaa tactgataat
     8641 gttgtcttaa cggcgattgc caacgatcgc ttacatgatg aagtgtatgc aaaacaggtg
     8701 cgggcgctgg gtcatgcggg agatgtattg ttagccattt ccacccgtgg caacagccgc
     8761 gatattgtaa aagcggtaga agctgccgtt acgcgtgata tgactattgt ggcattgacc
     8821 ggctatgacg gcggcgaact tgcaggtttg ttagggccac aagatgtgga gatccgcatt
     8881 ccttcgcatc gtagtgctcg cattcaggaa atgcatatgc tgacggtaaa ttgcctgtgc
     8941 gatctgatcg ataacacgct tttccctcac caggatgttt aaggagaata catgaaggca
     9001 ttatcgccaa tcgcagtcct tatttccgcg ctgctgttgc aaggttgtgt tgccgctgcc
     9061 gtagtgggta ccgctgctgt gggtaccaaa gccgcaactg acccacgcag tgtcggcacc
     9121 caggtggacg atggtaccct ggaagtgcgc gtgaacagcg cattgtcgaa agacgaacag
     9181 attaagaaag aagcccgtat taatgtcacg gcgtatcagg gcaaagtgct gctggttggg
     9241 cagtcaccaa acgctgaact ttcggctcgc gccaaacaga ttgctatggg cgtagacggt
     9301 gccaacgaag tgtataacga gattcgtcag ggccagccga ttggtctggg cgaagcgtct
     9361 aacgatacgt ggatcaccac caaagtgcgt tcgcagctct taaccagcga cctggtgaaa
     9421 tcgtccaacg tgaaagtgac caccgaaaac ggtgaagtgt tcctgatggg gctggtgact
     9481 gaacgtgaag cgaaagcggc ggcagatatt gccagccggg tgagcggcgt aaagcgggta
     9541 accacagcgt ttacgtttat taaatagcag ccccttgtaa tgcctgatgc gacgcttgcc
     9601 gcgtcttatc aggcctacaa cgatgcaaac cgtaggtcgg ataaggcgtt tacgccgcat
     9661 ccgacatgtt ttccctcaaa tcagaacaac agcgccagcc cgccgacaat cacaccagac
     9721 actgccacca tcgcccctgt cagccataag gctttcgctg ggaacgcttt gcgcagcata
     9781 atcagtgacg gcaaactcac cgccgggagc gtcatcaaca atgccagcgc cggagcagtt
     9841 cccatacctg ccagcatcat cgtttgtaca atcggaattt ctgctgccgt gggaatgaca
     9901 aacaagcatc ctgctaccgc catcgccatc acccacatca ggctgttatc gacagcacca
     9961 tcggcatggg ggaataacca gacgcgagcg gcacccaaca ccagtactgc aaggatgtaa
    10021 accgggatcg tactccagaa aagcgtccat agcgccctgc cccagcggct aaaaaaaccg
    10081 ccctgtgctt ccggtatgtc aatttcgacc ggtgcctgcg tttgcggtgt ttcacgcacc
    10141 catttttgca ccagcgtcgc aatcagcaac accatcacca gcccggccac cagacgaatc
    10201 gccgcaaaac cccagccgag gacaaacccc ataaacacca gcgtcgccgg gtttaacacc
    10261 ggattgccca tccagaatgc cagcgcaccg cccatcgaca cctgttggcg acgcattccc
    10321 gccgcgaccg gagccgcaca gcaggtacac atcatgcccg gcagcgaaaa cagcgttccc
    10381 agcagcgtgc cgcgaaagcg cgattgccca agcgtacgca acaaccagtc acgcgggatc
    10441 agcacctgaa tcaacgaacc gaggatcacc cccagcactg ccgctttcca taccgcgagg
    10501 aaatagagca tcgcgtaatc caacgccgcc tgccacggat tagcatccgc ctgcgcaagg
    10561 atagatttac cgatactgtg ggtttcggca gcagtaaacg ctttgccgta gtaaggttgc
    10621 catttcacat accagagacc ggcaatgaca acgagaaaga aaagcgcggg tttccaccac
    10681 tgaatgggcg ttgccgcctg agatgaagac tgaccagtca tagcattccc caggaataag
    10741 ttatgataat tgagcgcgtg aatattacgc tcactatcaa ttcttgggaa taattattca
    10801 gctcttttgc gtaattctga agagcttaaa atcgtcacac cttcatgccc gggccgcatc
    10861 gcctctgcca gcatgacccg cgccacgtct ctcgcatcaa tggatttcca gttaccgggt
    10921 aataaacgga acagcggcgc aaaaagcgtt tcgttcatcc gctgtttgct acgatcgccc
    10981 agtaacatcg acgggcgagc aatggtcaat ttcggccagt tctgggcgat taatgcttct
    11041 tccatctccc ctttgacgcg gttatagaaa aacggcgagt gggcattggc acccatagca
    11101 ctgaccacca gcatatgctg cgcgcccagt cgccgcccgg ttaatgcggt atccactacc
    11161 agcgtgtaat cggcatgaat aaacgcctct ttgctccccg cttctcgccg cgtggtgccg
    11221 agacaacaaa acacaatgtc gatgggatcg gtgacctgcg ccagcgcgtc agtcagttgc
    11281 ggatcatggg gattaaacac cccgggcata tcgcccaacg gacgtcgcgt cggcgcggct
    11341 atggcgttaa ctttcggttc gttaatcaac atccgcagca ggtgaccgcc caccagcccc
    11401 gttgcgcctg taatcagtac ctgactcatc ttcgctcctt tacagaattg tctgccttgc
    11461 gctccacggc tcatgcacca ggcttaatag accatgaggt aattatcccc gattgtggga
    11521 aattcgcctc atccaatgca acaacgcgga ggaagcatga gtaagaaaat tgccgtttta
    11581 atcactgatg aatttgaaga ttcagaattt acttcacccg cagacgagtt ccgtaaagcc
    11641 ggacacgaag tgattaccat tgaaaaacaa gcgggtaaaa cggtgaaagg caaaaaagga
    11701 gaagccagcg tgaccatcga taaatccatc gatgaagtga cgccagcgga gtttgatgcc
    11761 ctgctgctac cgggcggcca ttcaccggat tatctgcgtg gcgacaaccg ttttgtcacc
    11821 tttacccgtg attttgtgaa tagcggcaaa cctgttttcg ccatctgtca cggtccgcag
    11881 ctgctgatca gcgccgatgt gattcggggg cgcaaactga ccgcggttaa accgatcatt
    11941 attgatgtta aaaacgcggg cgcggaattt tacgatcagg aagtcgtggt cgataaagat
    12001 cagctggtta ccagccggac accggacgat ctgccagcgt ttaaccgcga ggcgttacgc
    12061 ctgctcggtg cctgagccgc gtagccaaat cattttttta ccaaagccca gcgtgttgtc
    12121 ggtgaatttg atttcatcga gccggatttc ccacaccggg gccgacagca ttctggcaac
    12181 cggaaagcga cgattgtacg ccttgcgcgc gaggtcgctt tcctcacctt ccagcctgcg
    12241 gatctcacct ttaaactgca cgccgcgaat taacgctacc gttttcggct gaccgtttac
    12301 cgttccggca accgcagcct gcggcccact catctgcgcg tggcgcgttt tttcttccgt
    12361 caaaatgtag aaggcgactt tctgcgcatc aaacagataa aaagcattag cgcaccagag
    12421 ttccccttcc tgctgcacac accaggtgac aacatgttgt tttgccaacc aacggctgat
    12481 ggtgatgagt gtttccattg ctgttctctt ttatactgtg ggcctgacct taacatgcga
    12541 cgcagacgat gacaccctgg tttctttact tgatccgtac cgccgacaat aagctttata
    12601 ccgggatcac cacggatgtc gaacgccgct atcagcagca ccaaagcggc aaaggggcga
    12661 aagcactgcg cgggaaagga gaactgacgc tggcgttttc tgcgccagtg ggcgatcgtt
    12721 cgctggcgtt acgggcggaa tatcgcgtta agcaactgac caaacggcaa aaagagcgtc
    12781 tggtagcgga aggcgcaggg tttgcagagc tgttaagcag tctgcaaacc ccggagatta
    12841 aaagcgattg aaatgctcgt gatactcaac cagaccagtt acgccattca acgcgtcatc
    12901 cgctaaacga tgtacctgga aggcgctttc agtgcctggc cagcggcaac gcaggtcgtg
    12961 atgtgccgcc agttcaaagc caaaacggct gtacagcgcc ggatcgccca gcgtcaccac
    13021 tgcggcatag ccgaactcat taagcgaatc gagtccttca tagaccagtt ggcgtgccag
    13081 cccctgtcca cggtattttt catcaaccgc caacggtgcc atgccgaccc attgcaggtc
    13141 ttcgccctgc acatcaaccg gactaaatgc cacatagcca atgacctgac cttcgtcatc
    13201 tgtcgccacc agccccagcg tcagaaagcc atcttcacgc agatcgtgaa ccagcttcgc
    13261 ttccgcatca ctttcgaatg agcgacgtag cagggcatca atacccggcg catcaatggg
    13321 aatttctact cgaattagca tggttcacct accgatgtct gtttggtttc aggcgcggtt
    13381 ttcattcccg cctcaacaaa atccgccagt tgcagcagca tcatgcgtaa ggctttcggc
    13441 atttgctcca gttcaatggc gtccatcagg tttttcacat acagccccag ctccgtatcg
    13501 ccttcaatca ccagccgacg ctggaagaag agcgtatccg gatcttgttt acgcgccgca
    13561 atcatcagca gatcgctggc gtcggcacta aaactcacat cagcttgcgc gttctggctg
    13621 accaccagtt tgccattcac caccgaggta aaccattgca ggtcaatatc acgcacatga
    13681 atacttaacc agcggccttc aagaaactcc agctcgccat catccagcgc ctgacggaat
    13741 tgccagctta agacctgctc aagaacctgg cgttttagcg caaatggcgt cagttttacc
    13801 ggtacactca acagagatgg ccccaaatgc acaatacggg aacgcagttt atccaacacg
    13861 agttttactc cctgtttcaa caatcatcct attttgccat atcagaaaaa taacatagcg
    13921 gtataaatca acaattccat atgaaattgc tgctaccacc aatacaactt taactgcctt
    13981 aaatcaaaaa ttgtcgcagc aaggttaact aaaatcccag ttcgttaaca tttttgcgtt
    14041 ttgatagcgc aaccttcagg aaaaattatg gagctgctct gccctgccgg aaatctcccg
    14101 gcgcttaagg cggccatcga aaacggcgca gatgctgttt atatcgggct aaaagatgat
    14161 accaatgccc gtcacttcgc cggccttaac tttaccgaga aaaaattgca ggaagcggtg
    14221 agttttgtcc atcaacatcg ccgcaaactt cacatcgcga ttaacacttt tgcgcatccg
    14281 gacggttacg cccgttggca gcgtgccgtg gatatggcgg cgcagctggg tgccgacgcg
    14341 ctgatcctcg ccgacctcgc catgctagag tacgccgccg agcgttaccc gcatattgag
    14401 cgtcatgtgt cagtgcaggc ttcggcgacc aatgaagagg cgattaactt ttatcatcgc
    14461 cattttgacg ttgctcgcgt ggtgctgccg cgcgtgttgt cgattcatca ggtgaaacaa
    14521 ctggcacggg tcacacctgt accactggaa gtctttgctt tcggcagcct gtgcattatg
    14581 tcggaaggtc gttgctatct gtcgtcgtat ctgacgggtg agtcgcccaa caccgtaggc
    14641 gcgtgttctc cggcccgttt cgtgcgctgg caacaaacgc cgcaggggct ggaatcccgc
    14701 ctgaacgaag tgctgatcga ccgttatcag gacggcgaaa acgcaggtta tccgacgcta
    14761 tgtaaagggc gttatctggt ggacggcgag cgctatcacg cgctggaaga accaaccagt
    14821 ctcaataccc tggaactgct gccggagtta atggcggcga atattgcttc ggtgaaaatt
    14881 gaaggccgcc agcgtagccc ggcgtatgtc agccaggtgg cgaaagtctg gcgtcaggct
    14941 atcgaccgtt gtaaggccga tccgcaaaac ttcataccgc aaagcgcgtg gatggagacg
    15001 ctcgggtcga tgtccgaagg cacgcagacc acccttggcg cgtatcaccg taaatggcag
    15061 tgagaagagc aatgaaatat tccttagggc cagtgctgtg gtactggcca aaagagacgc
    15121 tggaagattt ttatcagcag gccgccacca gcagcgccga cgtgatttat cttggtgaag
    15181 cggtatgcag caagcgccgg gcaaccaaag ttggcgactg gctggagatg gcaaaatcgc
    15241 tcgccggaag tggcaagcag attgtgctct ccacgctggc gctggtgcag gcatcctctg
    15301 aactgggcga actgaaacgc tatgttgaga acggtgagtt tctgattgaa gccagcgatc
    15361 tcggcgtggt gaatatgtgc gctgaacgca aactgccgtt cgtcgccggg cacgcgctga
    15421 actgctacaa cgcggtgacg ctgaaaatat tgctcaaaca gggcatgatg cgctggtgta
    15481 tgccggtgga gctttcccgc gactggctgg tgaatctgct taatcagtgc gatgagctgg
    15541 gcattcgcaa ccagtttgaa gtggaagtcc tgagctacgg tcatctaccg ctggcctact
    15601 ccgcccgctg ctttaccgcg cgttcggaag accgcccgaa agatgagtgt gaaacctgct
    15661 gcattaagta tccgaacggg cgcaacgtgc tgtcgcagga aaaccaacaa gtgtttgtac
    15721 tcaatggcat tcagaccatg agcggctacg tttacaacct cggtaacgag ctggcatcca
    15781 tgcaggggct ggttgatgtg gtccgcctgt caccgcaggg tactgacact ttcgcgatgc
    15841 tcgacgcctt ccgcgctaat gaaaatggcg ctgcgccact accgttgacg gcaaacagcg
    15901 actgtaacgg ctactggcgg cggctggccg ggctggagct gcaagcctaa gtaaatatgc
    15961 tcgtcatact tcaagttgca tgtgctgcat ctgcattcgt tcaccccagt cacttactta
    16021 tgtaagctcc tggggattca ctctcttgtc gccttcctgc aactcgaatt atttagagca
    16081 cagctcactt tgttaacaac tttaactact ctttaatgca gtattaaaga ttaatcggta
    16141 acaaagtgag ctgttatgac tgataaaacc attgcgtttt cgctactcga tctggccccc
    16201 attcccgaag gttcttcagc gcgagaagca ttctcccact ctctcgatct cgcccgtctg
    16261 gctgaaaagc gcggctatca tcgctactgg ctggcagaac accacaatat gactggcatt
    16321 gccagtgctg ccacgtcggt attgattggc tatctggcgg cgaataccac cacgctgcat
    16381 ctggggtctg gcggcgtgat gttgcctaac cactcaccgt tggtcattgc cgaacagttc
    16441 ggcacgctca atacactcta tccggggcga atcgatttgg ggctgggtcg tgcgccaggt
    16501 agtgaccaac ggacaatgat ggcgctgcgt cgccatatga gcggcgatat tgataatttc
    16561 ccccgcgatg tcgcggagct ggtggactgg tttgacgccc gcgatcccaa tccgcatgtg
    16621 cgcccggtac caggctatgg cgagaaaatc cccgtgtggt tgttaggctc cagcctttac
    16681 agcgcgcaac tggcggcgca gcttggtctg ccgttcgcgt ttgcctcaca cttcgcgccg
    16741 gatatgttgt tccaggcgct gcatctttat cgcagcaact tcaaaccgtc ggcacgactg
    16801 gaaaaaccat acgcgatggt gtgcatcaat attatcgccg ccgatagcaa ccgcgacgct
    16861 gagtttctgt ttacctcaat gcagcaagcc tttgtgaagt tgcgccgtgg cgaaaccggg
    16921 caactgccgc caccgattca aaatatggat cagttctggt cgccgtccga acagtatggt
    16981 gtgcagcagg cgctgagtat gtcgttggta ggtgataaag cgaaagtgcg tcatggcttg
    17041 cagtcgatcc tgcgcgaaac cgacgccgat gagattatgg tcaacgggca gattttcgac
    17101 caccaggcgc ggctgcattc gtttgagctg gcgatggatg ttaaggaaga gttgttggga
    17161 tagtgtgtct taacgcggga agccttatcc gagctggcaa cgctgtcc
//
LOCUS       DAZGJW010000096        17129 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZGJW010000096 DAZGJW010000000
VERSION     DAZGJW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.096"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            73..675
                     /gene="ais"
                     /locus_tag="ACHKIK_003262"
     CDS             73..675
                     /gene="ais"
                     /locus_tag="ACHKIK_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO9084380.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(714..1139)
                     /gene="nudI"
                     /locus_tag="ACHKIK_003263"
     CDS             complement(714..1139)
                     /gene="nudI"
                     /locus_tag="ACHKIK_003263"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO9084381.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1418..1960
                     /gene="yfaZ"
                     /locus_tag="ACHKIK_003264"
     CDS             1418..1960
                     /gene="yfaZ"
                     /locus_tag="ACHKIK_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO9084382.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2060..3262
                     /gene="yfaY"
                     /locus_tag="ACHKIK_003265"
     CDS             2060..3262
                     /gene="yfaY"
                     /locus_tag="ACHKIK_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO9084383.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            3326..3787
                     /locus_tag="ACHKIK_003266"
                     /pseudo
     CDS             3326..3787
                     /locus_tag="ACHKIK_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(3828..4730)
                     /gene="rpnE"
                     /locus_tag="ACHKIK_003267"
     CDS             complement(3828..4730)
                     /gene="rpnE"
                     /locus_tag="ACHKIK_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO9084384.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(4924..6114)
                     /gene="glpC"
                     /locus_tag="ACHKIK_003268"
     CDS             complement(4924..6114)
                     /gene="glpC"
                     /locus_tag="ACHKIK_003268"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO9084385.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(6111..7370)
                     /gene="glpB"
                     /locus_tag="ACHKIK_003269"
     CDS             complement(6111..7370)
                     /gene="glpB"
                     /locus_tag="ACHKIK_003269"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO9084386.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(7360..8988)
                     /gene="glpA"
                     /locus_tag="ACHKIK_003270"
     CDS             complement(7360..8988)
                     /gene="glpA"
                     /locus_tag="ACHKIK_003270"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO9084387.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9261..10619
                     /gene="glpT"
                     /locus_tag="ACHKIK_003271"
     CDS             9261..10619
                     /gene="glpT"
                     /locus_tag="ACHKIK_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO9084388.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            10624..11700
                     /gene="glpQ"
                     /locus_tag="ACHKIK_003272"
     CDS             10624..11700
                     /gene="glpQ"
                     /locus_tag="ACHKIK_003272"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO9084389.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(11742..11981)
                     /locus_tag="ACHKIK_003273"
                     /pseudo
     CDS             complement(11742..11981)
                     /locus_tag="ACHKIK_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            11979..12083
                     /locus_tag="ACHKIK_003274"
                     /pseudo
     CDS             11979..12083
                     /locus_tag="ACHKIK_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12163..12813
                     /gene="inaA"
                     /locus_tag="ACHKIK_003275"
     CDS             12163..12813
                     /gene="inaA"
                     /locus_tag="ACHKIK_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO9084390.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(12867..13121)
                     /gene="yfaE"
                     /locus_tag="ACHKIK_003276"
     CDS             complement(12867..13121)
                     /gene="yfaE"
                     /locus_tag="ACHKIK_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO9084391.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(13121..14251)
                     /gene="nrdB"
                     /locus_tag="ACHKIK_003277"
     CDS             complement(13121..14251)
                     /gene="nrdB"
                     /locus_tag="ACHKIK_003277"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO9084392.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(14340..16625)
                     /gene="nrdA"
                     /locus_tag="ACHKIK_003278"
     CDS             complement(14340..16625)
                     /gene="nrdA"
                     /locus_tag="ACHKIK_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO9084393.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            16753..16896
                     /gene="ypaB"
                     /locus_tag="ACHKIK_003279"
     CDS             16753..16896
                     /gene="ypaB"
                     /locus_tag="ACHKIK_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO9084394.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         4046 a         4180 c         4577 g         4326 t
ORIGIN      
        1 taattatcgc cagaaatgtc gctcaatttt acggtaattg tctggttgcg cttgtctata
       61 ggtggagttt acgtgttagc tttttgccgc tcatcattga agtcaaaaaa atatttcatc
      121 attttactgg cgctcgctgc aattgccgga ctgggtactc atgccgcctg gagtagcaat
      181 ggtttgccac gtatcgacaa taaaacattg gccagactgg cacagcagca cccggttgtc
      241 gttttgtttc gccatgctga acgttgcgat cgttcaacca atcaatgctt gtcagataaa
      301 acaggtatta cggttaaagg tacccaggat gcccgtgaac tgggcaacgc ttttagcgct
      361 gacatccctg atttcgatct ttattccagt aataccgtcc ggaccattca gtcggcgacc
      421 tggttttccg cgggtaaaaa gctgacggta gataaacgac ttcttcagtg cggtaatgag
      481 atttatagtg caattaagga cttacaaagc aaagcgcctg ataaaaatat cgttattttc
      541 acccataatc attgcctgac atatattgct aaaaataagc gtgatgcgac atttaaacct
      601 gattatctgg atggtttagt catgcatgtg gaaaaaggca aagtttatct ggatggggaa
      661 ttcgttaacc actaaatttt caatctgaca gccagtgatg gctgtcatca ttgttaaaga
      721 agacctttca aacgtaacgt ttttcgggtg gcaacattta aatcataatg caccagatct
      781 tcaggtttta cccacgcgta gtcctgaaac tcttcgttta ttttcacttc tcggttggca
      841 gaaacgcagt caaaaatcag gtaaatcata taaatctctt ccttgcgacc atctgcatac
      901 gtcttggtgc gaatatcatc gctgaaggtc cacggcgtga tttctgtcaa aagcagctgt
      961 tctcccagtt cttcgcgaat ttcgcggcgt agtgcctctt caattcgttc gccagactcc
     1021 acgccgccac ccgaaagcgc ccattgaccg gggaaaacgc cgcgatcgtc ggccatttta
     1081 cacagcaaat aagcaccatc attttgaatc aaagggcata caatagtccg ttgtcgcata
     1141 ggggtattcc ttataaataa aaagaaacca tgagaatacg gtaactcaga aatatgtgaa
     1201 agtgacagta atctcttttg caatgataac tgcacttatt attatttttg tgtgtatcgg
     1261 taaagtgtgt ttattttttc ggcgtttaat tatttaagaa gtatttagtt acaataagtg
     1321 actagatccc ttttttcgct cattgaatcc tctgtgccag gcagtacaat cccgcagctt
     1381 tgggaatttt gattaattgc ttgtgggaaa ggggataatg aaaaaaattg cgctagcagg
     1441 tctggccgga atgctgttgg tttctgcatc ggtcaatgca atgagcatca gcggccaggc
     1501 gggtaaagaa tacaccaata ttggtgtcgg ttttgggact gaatcgacgg gcctggcttt
     1561 aagcggtaac tggacacata acgacgacga cggtgacgtc gcgggcgtgg ggctggggtt
     1621 gaatctgcct ctcgggccgt taatggcgac cgttggcgga aaaggcgttt acaccaaccc
     1681 gaattacggc gatgaaggtt atgccgcagc ggtaggaggt ggtttgcagt ggaaaattgg
     1741 caacagcttc cgtttgtttg gcgagtatta ctactctccg gattcgctct ccagcggtat
     1801 tcaaagttat gaggaagcga atgctggcgc gcgttacacc attatgcgtc cagtcagtat
     1861 tgaggcgggt tatcgctacc tgaatctgtc gggtaaagac ggtaaccgcg acaacgccgt
     1921 ggctgacggc ccgtatgttg gggttaacgc cagtttctga tcccttccgg cgcggcattc
     1981 tgtcgcgcca ttcagtttct tactacgcct tatctccctt tgcgttatag tgattcgata
     2041 acaaaagcag gagataacca tgttaaaagt ggaaatgcta tccaccgggg atgaagtgtt
     2101 acacgggcaa atcgttgaca ctaacgctgc ctggctggcc gattttttct ttcatcaggg
     2161 gctgccatta tctcgccgca atacggtggg ggataacctt gatgacttag tcaccattct
     2221 tcgcgaacgt agtcagcacg ccgatgtgct gatcgttaac ggcgggctgg gaccgaccag
     2281 cgatgattta agcgcactcg ccgctgcgac agcaaaaggt gaaggcatgg tgctgcatga
     2341 agcctggctc aaagagatgg aacgctattt ccacgaacgt ggacgagtaa tggcaccgag
     2401 caaccgtaaa caagcggagc tgcctgccag tgctgaattt atcaataacc cggtaggcac
     2461 cgcctgtggt tttgccgtgc agcttaatcg ttgcctgatg ttctttactc ccggcgtacc
     2521 gtcagaattt aaggtgatgg tcgagcacga aatcctgccg cgtctgcgcg agcgtttttc
     2581 tttaccacag ccgccggttt gtctgcgttt aactaccttt ggtcgttcgg aaagcgatct
     2641 ggcacaaagc ctggacactc tacaactgcc gccgggcgta acaatgggct atcgctcttc
     2701 aatgccgatt atcgaactga aactcaccgg accggcaagc gagcaacagg cgatggaaaa
     2761 actgtggctg gacgttaaac gtgttgccgg acagagcgtg attttcgaag gcaccgaagg
     2821 actgcccgcg cagatcagtc gcgaattgca aaaccgccag ttcagcctga cgttgagcga
     2881 gcaattcacc ggtggtttat tggctttgca actttctcgc gcaggtgctc cattgctggc
     2941 gtgtgaagtg gttccttcac aggaagaaac cctggcgcaa actgcgcact ggattacaga
     3001 acggcgggcc aaccattttg ccgggctggc actggctgtt tcgggtttcg agaacgaaca
     3061 tctcaacttt gcgctagcca caccagacgg cactttcgct ctgcgtgtgc gtttcagcac
     3121 tacgcgctac agcctggcta tccgtcagga agtgtgcgca atgatggcac tgaatatgct
     3181 gcgccgttgg ttaaacggcc aggatatcgc cagtgagcat ggctggattg aggttattga
     3241 gtccatgacc ttatctgtct gattccgcca ctctgtgccg gtttactgca ccggcacatt
     3301 ccccctccgg caaaacttgt ccaaccctcc ctacggtgag cgtggtgttg gggccagtgt
     3361 ggcacgggct gcgcgctggg gacgcattga gaattacatg gcgcaagtca acgattcgct
     3421 ttgtctgtta gtgcaggtgg aaagtaaaac ggcactggat aacctggacg agatcctcga
     3481 cgtcgaaggg attgatggcg tgtttattgg accagcggat ctttctgcgt cgttgggcta
     3541 cccggataac gccgggcacc cggaagtgca gcgaattatt gaaaccagca ttcggcggat
     3601 ccgtgctgcg ggtaaagcgg ctggttttct ggctgtggct cctgatatgg cgcagcaatg
     3661 cctggcgtgg ggagcgaact ttgtcgctgt tggcgttgac acgatgctct acagcgatgc
     3721 cctggatcaa cgactggcga tgtttaaatc aggcaaaaat gggccacgca taaaaggtag
     3781 ttattgatat caaaggccca tggggatcgg ctgtgggcct gtgttaatta gtggttattc
     3841 gctgccagat cggcttcgct tagctgggtg gccgcgagca cctggtcacg atcaatgccc
     3901 tgttccagca tgcgcaaagc aattttaatg gcttgttcat gcataccttc ctgccagcca
     3961 atttgatgcc cttcctgccg tagccgttca gcaatagtca ttaatctctc cttttgtagt
     4021 ggtgaacgtt cggcaatctc ctcaataaaa cgggtgaaac gggaggtatc gccgcattgc
     4081 agcagataat taaacagtgt ttgtagctgg ctgtcattag tgaagcctct aaccaaaagc
     4141 gtggtgatcc tgtcgaccat gccgattaaa tcgcggtcgc gaatatgctt ttgaatcagt
     4201 tccagcagag ctatacgccg atgttgcatg atctcatcgt caggtacgat ggtgatatcc
     4261 accaacggaa aagcttcggt gtacaactgc cgggcaagtt gcggatcgtc aaactcatcc
     4321 agccagttaa gtgagtacgg gtagggcgat gtttcgccat gataaaacag caatggcacc
     4381 accagcggaa ctctgtcata gcctttatcc aggtgacgct gcatggcggc agtggcatag
     4441 cgcattagcc gaaaagccat attcttttct gcagagcttt gatgttcaat cacgcagtag
     4501 atatagccgt caccgtcgct ggtttccacg gaccacaaaa catccgagta gtaagcgcgt
     4561 aaactttttt caataaaact agtgggttcc aggcgtaagg tttgcaggtt gcaaagcttg
     4621 cgcagtggtt ctggtaaatg tatttcgaga aaatcccgtg cggtttcggg tgtgaacata
     4681 aaggttttaa acaccgcatc atgcggggag gatgttgttg attcggtcat ggttttccgt
     4741 cacctgatat ttatgatgac gcgacattac gtctatcccg ggcctttgac atctgtcagt
     4801 ttcagccttt gcgagacatt ttcagaggaa ttttcaaagc gttgcaacgc atacatggtg
     4861 cgtggaatcg cgctcgcaaa aatgcccgat aagcaatgtg cttaccgggc atcaaaaggt
     4921 cggttaagcc agcgcctggg ccagtagcgt aatcggatgt tcgcagcgaa ggctcgtgga
     4981 catctcaatc tgccatttgc aggtttcgca gtcggtgatg accagatccg cgccgctctc
     5041 ttctatctgg cggaacagtg gcgcaccgat ggcttgtgag gtggggtagt tctctttttt
     5101 gaaaccgtaa gtacccgcaa taccgcagca ctgggaatcc agcaccgtta actcaagccc
     5161 cgggatttta cgcaacagct ccagggtgta gagcgtccag cccatttttt ccatatggca
     5221 cggagtgtga taaaccactt tcagcggcag cggtttcagc ggtaacgttt tgccttcgtc
     5281 cagcttgcgc cacagccagc gggttgccag ttcgatatga tcgcgcaagc ctttgttgtc
     5341 gacattcagc acttccgggt attcgtcgcg cagggcaaat gtacaggttg aggaggtggc
     5401 aatcaccgga atgcctttta ctcccacagc ttcgcggatc gactctacat tcgtaattgc
     5461 ctgtttgcgt gctttatcgg taaagccgtt ggcgattagc ggtacgccgc agcatttttc
     5521 tttgctgagc agttgtacac cggtacccat tgcgttgagc actttaatta aatctttacc
     5581 taactgcgga tggttgtagt taacgaagca gccgtgaaag aaagcgacct ggtctttata
     5641 ttgtgcttgc tgagccgcta tgctgcgata ccagcgacga aacgtaccga aggagtattt
     5701 cggtagcgtg cggcgatgat cgatttttaa cgccgcatca agcagctgcc gcaccggttt
     5761 cagcgaggta gcggtgttga cgattggtgc gaacggcgtg gaaacgctac ccatcaggtc
     5821 ggtatgactc aacacaaaat tacgcagcga cgggcgcgtg gtgtcatatt ttgcccgcgc
     5881 gcgctggata atatcgccaa tcttcacatc ggacgggcag gcgacttcac aacgtttgca
     5941 gttgatgcaa tatttcagcg cctcgtcata cagtgcgcca tctttcaaac gcagacgctc
     6001 gccatccggc ccggcttgtt ttggccctgg ataaccggga ttcacccggc tcaccgggca
     6061 ggcggtggtg cagacggtgc acttaatgca gttttcgaag ctggtgtcgt tcattgttgg
     6121 cctcctgcgc gttgggcaat ctgttgagcg gcatgtaaag cactgacggc acaaacaccg
     6181 ccgccgcatc cctgggcgat gggatcaaat ccgcccagca ccgaaccgat ggcaaacagg
     6241 ttctcaatgg tttgccctgc ctgcgacggg cgtagcgtct gatcagtggt tacaccgaac
     6301 tgctgccacg gttgcggcgc aaaaaaatcg cccttatacc attcaccccg cgtggcggtt
     6361 tgtagcacat caaggccgag aatcggctcg cgaatgccgt tacgttccgc taccagtccg
     6421 ccactaaaga aactgccgct ggcgagaacc gcgaaacgtg gacgtagcgg aatatcggcg
     6481 tgattgcggg tccagatttc attcactacg ccatttttac aggtcacttt tttcacttca
     6541 tcgcccggca tccacacgcc accctggcgc acaaactggc gctgtaactg gttttgcaga
     6601 cgaatgccca gcacggaagg cggcagcgtt ggcaaaagca tcagtgaaca aggtagtttt
     6661 tcattcaacc aacgccacag tttgtcatcg gccaggccga agcaggcggg catcaggatc
     6721 atttcgcagg tattggcgac aggaataagc gcatcaagta acagcggcca gttttcttca
     6781 ttatcaagga aacgggcgat attcaccgcg cgaaattcgg tggcgttatt gcgcaggaca
     6841 tccagttccg gcagctctat ttctgcggtt tcaaccgaaa gatccagttc gcgcaacgaa
     6901 gccgccgcaa gatgcgcctg gaaatccatc agcccgctta tccccactac acatatcttc
     6961 ttcgcgggta gcggccagac gggaacttct ggcgaactta accaggttgc acgcaaagtg
     7021 ccgagcgggg taacgcgctg atgggccagt tccacgctgc cctgcaattg cgcaccgctt
     7081 tcagcgatca atgcctgcgc ctggcaagcg agatcgagca cgcgttgtgg ttcgagaaga
     7141 ctgtaaggat gggctggtgc ctgctgacgc aaagattcca gtccactgtg aatgtctgtc
     7201 accggttgac catcaggcag atggctcagc aaatccagcg aaccggatga gaaatgcagc
     7261 gcgctttgac cacgcgtgac aatggcacag cgcaggccgt gtttttgcag ttgcaggcca
     7321 cagagtaatc cggcgaggcc gccgcccata atgacagtat caaagcgcat ctttctgctc
     7381 cttctccaga ccacacaatc cctgataaac ccagcgggta aattcgcttt cgcgcagtgc
     7441 atctccccag gcgatgggtt gcacgccttt ccagcgttcg ttaaggaagg tggaaagttg
     7501 ctcgatagat tgcgcggacg tcgtgacgtt aaaacgttgc agcagtccgg cagcgcggca
     7561 ggcgcagagt tcgccctggc aggtacccat ccccacacgg gtacgacggc gtaaatccag
     7621 caggctatta acgtttaaat tttctaccgc gtactgaact tcacccgcag ttaccgcttc
     7681 gcactcacat accaggctac gatgcagacg gccttcgctc agccaggcag gcgtgcgatc
     7741 gccatgacga taaaccgcag aaccgcgcag cggggcaggc agggagatga ctttacgcaa
     7801 ggtaacttca gcgggttctt gtgaaccagg cagtgccaga tcggcagtcg tacaggggcg
     7861 cgtgttgccc agtttgcggc ataccgcgtc ggtagcccat tcagccatca gccgataggt
     7921 catcagtttg ccaccggtga tggtgataaa tccgtccaga ccatcgcgtt cagcatggtc
     7981 gagcagcacg atgccacggc tgacgttacg tccgctcggg tcgtcatcgc tggcaaccag
     8041 cgggcgcacg ccagaatagg cccgcaaaat gcgcgttttc gccatcacgg gggccagttt
     8101 ttccccttca cgcagcagaa tatcaacctc ttctgccgtc actcgattat cgtcaatctc
     8161 gttgtaatca atacgtaaag aggtggtgcc aatcagcgaa atggtatcgc caggcaccag
     8221 aatatcggcg tcggaaggtt tacggcagcg gttgatcaca tgctggttaa tgcggtgatc
     8281 catgatcagc agcgatcctt tcgccgggaa catgcgaatg cgcagatcgg catattcggc
     8341 aatgtgttgc ccccagatcc cagcggcatt aaccacgaca ggtgcatgaa gggcctgagt
     8401 ttcgccggtg agatggttac gtacacgaac accgcacacc gtcgcgcctt cacgaatcag
     8461 ccccgtgact tcatgagcgg taaggataac ggcaccgtgt tctttggcat ccagcatgtt
     8521 tgctgcggtc agacgaaatg gatcaacggt gccatccgga actttcaccg cgccaatcag
     8581 tgccgggtta acggcaggtt cgataatgcg cgcttgctgc gggtctatag cttctgcgct
     8641 gatccctgct tcttcgcagg cgcgaataaa agtggcctgg aatgagaggt catcttccgg
     8701 cagggtgata aacaggccgt tggttggttc aacgcagtga cgtgcaatgc gtttcaggat
     8761 ctggttttca ctaatgcatt cgcgggccga ttccgcatcg gttaccgcat agcgcgcacc
     8821 gctgtgcagc aggccgtggt tacgcccggt ggcaccggtt gcgatgtcgt ggcgctcaac
     8881 caaaatcacg cgcagcccgc gcagggcaca gtcgcgggca atcccggctc ccgttgcgcc
     8941 gccaccaatg ataatcacgt cacttgattg cgagtcgcga gttttcattg ttatccctct
     9001 gaagttcgtt ttttaccatt tagccatagt aaaaacatga attgtttgat ttcgcgcata
     9061 ttcgctcata attcgaaagt gaaacgtgat ttcatgcgtc attctgaaca ttttgtaaat
     9121 cttatttaat aatgtgtgcg gcaattcaca tttaatttct gaatgttttc ttaacatcgc
     9181 ggcaactaaa gaaacggcag gttctcccac tgaatcagtc tgttaatcat aaataagacc
     9241 acgggccacg gaggctttca atgttgagta tttttaaacc agcgccacac aaagcgcgct
     9301 tacctgctgc ggagatcgat ccgacttatc gtcgattgcg ctggcaaatt ttcctgggga
     9361 tattctttgg ctatgcggct tactatttgg ttcgtaagaa ctttgcgctt gctatgcctt
     9421 atctggtcga gcagggattc tctcgcggtg atttaggttt cgcgctttcg gggatctcga
     9481 tcgcttatgg attttcgaaa ttcataatgg gttcggtatc ggatcgctcg aatccgcgcg
     9541 ttttcctgcc cgcaggtttg attctggcgg cggcagtgat gttgtttatg ggctttgtgc
     9601 catgggcgac gtcgagcatt gcggtgatgt ttgtactgtt gttcctctgc ggttggttcc
     9661 aggggatggg gtggccgccg tgtggtcgta ctatggtgca ctggtggtcg cagaaagaac
     9721 gtggcggcat tgtgtcagtg tggaactgtg cgcacaacgt cggtggtggt attccgccgc
     9781 tgctgttcct gctggggatg gcctggttca atgactggca tgcggcgctc tatatgcctg
     9841 ctttctgcgc cattctggtg gcattattcg cctttgcgat gatgcgcgat accccgcaat
     9901 cctgtggctt gccgccgatc gaagagtaca aaaatgatta tccggacgac tataacgaaa
     9961 aagcggaaca ggagctgacc gcgaagcaaa tcttcatgca gtacgtgctg ccgaacaaac
    10021 tgctgtggta tatcgccatc gccaacgtgt tcgtttatct gctgcgttac ggcatcctcg
    10081 actggtcacc gacttatctg aaagaggtta agcatttcgc gctggataaa tcctcctggg
    10141 cctacttcct ttatgaatat gcaggtattc cgggcactct gctgtgcggc tggatgtcgg
    10201 ataaagtctt ccgtggcaac cgtggggcaa ccggcgtttt ctttatgaca ctggtgacca
    10261 tcgcgactat cgtttactgg atgaacccgg caggtaaccc aaccgtcgat atgatttgta
    10321 tgattgttat cggcttcctg atctacggtc ctgtgatgct gatcggtctg catgcgctgg
    10381 aactggcacc gaaaaaagcg gcaggtacgg cagcgggctt taccgggctg tttggttacc
    10441 tgggcggttc ggtggcggcg agcgcgattg ttggctacac cgtggacttc ttcggctggg
    10501 atggcggctt tatggtaatg attggcggca gcattctggc ggttatcttg ttgattgttg
    10561 tgatgattgg cgaaaaacgt cgccatgaac aattactgca aaaacgcaac ggaggctaat
    10621 ggcatgaaat tgacgctgaa aaaccttagc atggcgatca tgatgagcac tatagtcatg
    10681 ggaagcagtg caatggcggc ggacagcaac gaaaaaatag tcatcgccca tcgcggtgcc
    10741 agtggatatt tgccggagca tacgctgcca gcaaaagcga tggcgtatgc gcagggagcg
    10801 gattatctgg aacaggattt ggtgatgacc aaagacgacc atctggttgt tctgcatgac
    10861 cattatctcg atcgtgttac tgatgttgcc gatcgtttcc cggaccgggc gcgcaaagac
    10921 ggtcgttact acgcgataga tttcacgctg gatgaaatta agtcgctgaa atttaccgaa
    10981 ggtttcgata ttgaaaacgg taaaaaagtg cagacttatc cggggcgttt cccaatgggt
    11041 aagtccgact tccgggtgca cacctttgaa gaagagattg aatttgttca ggggttaaat
    11101 cactctaccg ggaaaaatat cggtatttat cctgaaatca aagcgccgtg gttccatcat
    11161 caggaaggga aggatattgc ggcaaaaacg ctggaagtgc tgaagaaata tggttacacc
    11221 ggtaaggacg acaaagttta tttgcaatgt tttgatgctg atgagctgaa gcgtattaag
    11281 aatgagctgg aacctaaaat gggcatggat ctcaatctgg tacagttgat tgcctatacc
    11341 gactggaatg aaacgcagca gaaacagccg gatggaagct gggttaatta caactacgac
    11401 tggatgttta agccgggtgc tatgaaacag gtggcggaat atgccgacgg aatcgggccg
    11461 gattaccata tgttgattga ggaaacatcg cagccgggta atatcaaact cactggcatg
    11521 gtgcaagatg ctcagcagaa taaactggta gtgcatcctt ataccgtgcg gtcagataaa
    11581 ctgcctgaat acactactga tgtgaatcag ttatatgatg ctctgtataa caaagcgggt
    11641 gtaaatgggc tgtttactga tttccctgat aaggcagtaa aattccttaa taaagagtaa
    11701 tgctactgca ggatggcgtt catgcgccat cctgtctcac atcaaatttt tccattcacc
    11761 acgagttgtt ccgtacacgt ttgtagtaaa aacagtggac tgccttctac cggcttttct
    11821 ttagccaaca acgaaatctg tcgatagaag gttggttcgt gaggaacgga acacaattca
    11881 cctgcaatgc ttttcagcgt taactctggt tgtaaggcga tgcctaatcc ctgtcgaata
    11941 aacttcatcg ctgtatccga ctgattaaat tcacatttta tctactgggc gcactattag
    12001 tggtagtggc tggtggaatc tatgcgctgc aaatgaaggg taaaagcggt gtcacgctag
    12061 tagtggcaaa agaaattcat taatacgaca cgtttcatta agattttcct caggtaacca
    12121 cctatagtca ttgtcgttaa taaaaatgac aggcgatagg gtatggcagt ttcagcaaag
    12181 tacgacgaat ttaatcactg gtgggcaaca gagggcgact gggttgaaga gcctaactat
    12241 cgccgtaatg gtatgagcgg ggtgcaatgc gtcgagcgca acggcaaaaa gctgtatgta
    12301 aagcgcatga cgcatcatct gtttcattcc gtacgttatc cgttcggccg accaacgatt
    12361 gtccgtgagg ttgcggtaat taaagaactg gaacgggccg gtgtcatcgt gccgaagatc
    12421 gtttttggtg aagcggtgaa aattgagggt gaatggcgag cgttgctggt gactgaagat
    12481 atggcggggt tcatcagcat tgctgactgg tatgcccagc atgcagtatc gccttattct
    12541 gacgaagtac ggcaagccat gttgaaagca gtggcgctgg cgtttaagaa aatgcatagc
    12601 attaatcgtc agcatggctg ttgttatgtt cgccatatct atgtgaaaac agaaggtaat
    12661 gcagaagctg gttttcttga cctggagaaa agtcgtcgcc gcttgcgtcg cgataaagcg
    12721 attaatcatg acttccgcca gctggaaaaa tatctggagc caatccccaa agcggactgg
    12781 gagcaggtga aagcgtatta ctacgccatg taatcgttgt aggccagata agacgcataa
    12841 gcgttgtatc tggcactaca accaattcac atctcgattt caatatcgcc ttttgcccgg
    12901 caacaacagg gcaaaatttc ccccggctga ataaaggcta acggttcggc aatccagtca
    12961 acctgacctg caaccagacg ggtgcgacag gagccgcagt aaccttcgcg acactgatac
    13021 tcaaccgcca cattgtggga ttccagcgcc gccagaaggg aagggtgttc atcctggcac
    13081 agcagttgtg tgccagtgat gcgcagggta acgcgggcca tcagagttgg aagttactca
    13141 aatcgtcggt gtccacttcc gcgtcaatct gcccgaccag ataagaactg acttccactt
    13201 cctgcggagc aacctgcacg ttatcagaca ccagccaggt gttgatccat gggatcgggt
    13261 tggagcgcgt ctggaacggc agatccaaac cgactgcctg catacggata ttggtgatgt
    13321 attcaacgta ctggcagaga atgtctttat tcagaccaat catcgaaccg tcgcggaaca
    13381 gataatccgc ccagtctttc tcctgttgag ctgcctgaac aaacaggtca tagcactcct
    13441 gcttacactc ttcggcaatt tccgccatct caggatcgtc cgcgccgctg cgcagcagat
    13501 tcagcatatg ctgggtgccg gtcaggtgca gggcttcgtc gcgggcaatc aggcgaataa
    13561 ttttggcgtt gccttccatc aattcgcgtt ctgcaaatgc gaaggaacaa gcaaagctga
    13621 cgtagaaacg aatcgcttcc agcgcgttaa cgctcatcag gcagagatac agttttttct
    13681 tcagctcgcg caggctaacg gtcacagttt taccgttaac ggtgtgggta ccttcgccca
    13741 gcagatgcca gtagctggtc atttcgatca gctcatcgta atagctggag atcccttccg
    13801 cacgtttctg gatctgctcg ttggtgacga tatcgtcaaa cacaacagac ggatcgttaa
    13861 cgatattacg aatgatatga gtataggaac gggaatgaat cgtttctgag aacgcccagg
    13921 tttcgaccca ggtctccagt tccgggatcg agatcagcgg cagcagtgcc acgttcgggc
    13981 tacggccctg aatggaatcc agcagcgtct gatatttcag gttgctgata aagatgtgtt
    14041 tttcgtgttc aggcagtgcc tggtaatcga tacggtcgcg agaaacgtca acttcttccg
    14101 gacgccagaa gaaagagagc tgcttttcga tcagcttttc gaagatgtca tatttttgct
    14161 gatcgtagcg agccacgttg accggctgac caaagaacat cggttctttg agctgatcat
    14221 ttttcgtctg tgaaaaggtg gtatatgcca tgagtgtgtc ctgtggtttt attacccctc
    14281 accctaaccc tctcccgcaa ggggagaggg gacggtttgg tgcaaaggct gggggattat
    14341 cagatcttac atgcgccgct ttcgcagcca tcgtcctgga ttgacggtac cagatcgtcc
    14401 tgcgcgtctt cagcgccgtc acgggtgttc tgataataca gcgttttgac cccgaatttg
    14461 taggcggtga gcaggtcttt cagcaactgc tgcatcggca cttttcctga cgggaaacgt
    14521 gacggatcgt agttggtgtt agcagagatc gactgatcga taaatttctg catgatacct
    14581 accagttgca ggtagccatc gttacccggc atttcccaca gcagctcata ggcgtcgtgc
    14641 aggtgctcgt agtccggcac cacctggcgc aaaataccgt ctttcgacgc tttgatgctg
    14701 acgtaaccgc gcggcggttc aataccgtta gtggcgttag agatctgtga agaagtctct
    14761 gacggcatca gggcagaaag cgtggagtta cgcaggccgt gcgttttgat tgattcacgc
    14821 aatgcttccc agtcgtaatg cagcggctca ttagcgatgg tatccagatc tttcttatag
    14881 gtatcgatcg gcaggatccc tttcgcgtaa gtggtttcgt taaaccacgg gcacgcgcct
    14941 tgctcttttg ccagctcgtt agacgctttc agcaggtaat actgaatcgc ttcgaaggtt
    15001 ttatgcgtca ggttgttggc gctgccgtcg gagtaacgca caccgtgctt cgccaggtag
    15061 taagcgaagt tgatcacacc aatacccagc gtacgacgac ccatcgctcc acgtttggcg
    15121 gccgggatcg ggtaatcctg ataatccagc agcgcgtcaa gtgcacgaac cgccagaatt
    15181 gccagctctt ccagttcatc caggctatta attgcgccca ggttgaaagc agacagcgta
    15241 cacagcgcga tttcaccgtt ctcgtcgttg acgtcgttca gcggtttggt cggcagggct
    15301 atctccaggc acaggttaga ctgacgcact ggcgcgatgg ccggatcaaa cgggctatgg
    15361 gtattgcagt ggtcaacgtt ctgaatatag atacgaccgg tagacgcacg ttcctgcatc
    15421 atcagcgaga acagctcaac ggctttcaca cgctgcttgc ggatgctgtc gtctttctca
    15481 tatttggtat acaggcgttc aaactcttcc tgatcggcga agaatgcgtc gtacagcccc
    15541 ggtacgtcgg acgggctgaa cagggtgata tcttcacctt tcagcaggcg ggtatacatc
    15601 agtttgttga tctgtacccc gtagtccata tggcgcacgc ggttgccttc cacaccacgg
    15661 ttgtttttca acaccagcag gctttccact tccagatgcc acatcgggta gaacagcgtt
    15721 gccgcaccgc cgcgcacgcc gccctgagag caggatttca ccgctgtctg gaaatgtttg
    15781 tagaacggaa tacagccggt atggaacgct tcaccaccgc gaatcgggct acccagcgca
    15841 cgaatacgcc cggcgttgat gccgatcccg gcacgctggg aaacgtattt cacaatcgcg
    15901 ctggaggtag cgttgatgga atccagactg tcaccgcact cgatcagtac gcaggagctg
    15961 aactgacgag tcggggtacg cacgccggac atgattggcg tcggcagcga aattttaaat
    16021 gtggaaaccg cgtcgtaaaa acgcttcaca tattgcaggc gcgtttcacg cgggtagttc
    16081 gagaacaggc acgcggcaac cagaatataa aggaactggg cgctttcgta gatttcgccg
    16141 gtcacgcggt tctgtaccag atatttgcct tccagctgct taacggcagc ataagagaag
    16201 gtcatatcac ggtcgtgatc gataaacgtg tccatctgct tgaactcttc ttccgtgtag
    16261 tcttccagca gatgattatc gtatttgccc atctcgacca ttttcaccac gtggtcgtac
    16321 agcgcaggcg gctcaaactg gccgtaggct tttttacgca ggtggaagat cgccaggcgc
    16381 gcggcgagat actgataatc cggcgcatca cgggagatca ggtctgcggc cgccttgata
    16441 atggtttcgt ggatgtcaga ggtcttgata ccgtcataaa actgaatgtg ggagcgcagc
    16501 tcgacctggg aaatcgaaac gttatgcagt ccttctgccg cccaatccag aacgcgatgg
    16561 attttgtcga gattgatgcg ctctgtgcta ccgtcgcgct ttgtcaccag cagattctga
    16621 ttcatgtatg tcgtacctgt ttttggaatt ctttccgcaa tagtttcatg cagactacct
    16681 gtagtacggg aagctgctta gaacactata tatagggggt atgtttggga ttcactgcaa
    16741 gatagtgtga aaatggccct cttgcaagtg cataactttg tggataactc aggaaggaaa
    16801 aagtggcttt cgcgcacctt aggtcagaca aggtgtccgg gaaagtcaat gggaagagaa
    16861 aaatttgtta aaaataactg ttcggcgatt tcttgaacgg cagaataggt tggataaatt
    16921 gatatagatg gtttattaat ctttttgcta atgattgcaa aaattgatct aagcacgagt
    16981 catcacataa tctgatgtat cagttgtggt caatatataa gcgaatgtaa gcgattgttg
    17041 atgtcgaaat ggttgttttc tattcggtta ttgttaaatg cgaagccatt ttgttaattt
    17101 accttatatt ataagaaaat tattcatta
//
LOCUS       DAZGJW010000097        17111 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZGJW010000097 DAZGJW010000000
VERSION     DAZGJW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.097"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            25..819
                     /locus_tag="ACHKIK_003280"
     CDS             25..819
                     /locus_tag="ACHKIK_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO9084395.1"
                     /translation="MTLPTTIYSFPAYSPHFSSTDKPVKLKFHQYARATLLPNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHKATLDNIHAQKY
                     NKAHAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNS
                     AAIYSGLGFERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(890..1339)
                     /gene="rplI"
                     /locus_tag="ACHKIK_003281"
     CDS             complement(890..1339)
                     /gene="rplI"
                     /locus_tag="ACHKIK_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO9084396.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1381..1608)
                     /gene="rpsR"
                     /locus_tag="ACHKIK_003282"
     CDS             complement(1381..1608)
                     /gene="rpsR"
                     /locus_tag="ACHKIK_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO9084397.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1613..1927)
                     /gene="priB"
                     /locus_tag="ACHKIK_003283"
     CDS             complement(1613..1927)
                     /gene="priB"
                     /locus_tag="ACHKIK_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO9084398.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1934..2329)
                     /gene="rpsF"
                     /locus_tag="ACHKIK_003284"
     CDS             complement(1934..2329)
                     /gene="rpsF"
                     /locus_tag="ACHKIK_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO9084399.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2656..2931
                     /gene="yjfY"
                     /locus_tag="ACHKIK_003285"
     CDS             2656..2931
                     /gene="yjfY"
                     /locus_tag="ACHKIK_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO9084400.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(3060..3746)
                     /gene="ulaF"
                     /locus_tag="ACHKIK_003286"
     CDS             complement(3060..3746)
                     /gene="ulaF"
                     /locus_tag="ACHKIK_003286"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO9084401.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(3746..4600)
                     /gene="ulaE"
                     /locus_tag="ACHKIK_003287"
     CDS             complement(3746..4600)
                     /gene="ulaE"
                     /locus_tag="ACHKIK_003287"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO9084402.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(4610..5260)
                     /gene="ulaD"
                     /locus_tag="ACHKIK_003288"
     CDS             complement(4610..5260)
                     /gene="ulaD"
                     /locus_tag="ACHKIK_003288"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO9084403.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5274..5738)
                     /gene="ulaC"
                     /locus_tag="ACHKIK_003289"
     CDS             complement(5274..5738)
                     /gene="ulaC"
                     /locus_tag="ACHKIK_003289"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO9084404.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(5748..6053)
                     /gene="ulaB"
                     /locus_tag="ACHKIK_003290"
     CDS             complement(5748..6053)
                     /gene="ulaB"
                     /locus_tag="ACHKIK_003290"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO9084405.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(6069..7466)
                     /gene="ulaA"
                     /locus_tag="ACHKIK_003291"
     CDS             complement(6069..7466)
                     /gene="ulaA"
                     /locus_tag="ACHKIK_003291"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO9084406.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGYIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            7821..8885
                     /gene="ulaG"
                     /locus_tag="ACHKIK_003292"
     CDS             7821..8885
                     /gene="ulaG"
                     /locus_tag="ACHKIK_003292"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO9084407.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            8993..9748
                     /gene="ulaR"
                     /locus_tag="ACHKIK_003293"
     CDS             8993..9748
                     /gene="ulaR"
                     /locus_tag="ACHKIK_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO9084408.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(9745..10494)
                     /gene="yjfP"
                     /locus_tag="ACHKIK_003294"
     CDS             complement(9745..10494)
                     /gene="yjfP"
                     /locus_tag="ACHKIK_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO9084409.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITTRHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            10676..11005
                     /gene="bsmA"
                     /locus_tag="ACHKIK_003295"
     CDS             10676..11005
                     /gene="bsmA"
                     /locus_tag="ACHKIK_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO9084410.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11154..11429
                     /gene="yjfN"
                     /locus_tag="ACHKIK_003296"
     CDS             11154..11429
                     /gene="yjfN"
                     /locus_tag="ACHKIK_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO9084411.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(11546..13171)
                     /locus_tag="ACHKIK_003297"
     CDS             complement(11546..13171)
                     /locus_tag="ACHKIK_003297"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO9084412.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13255..14418)
                     /locus_tag="ACHKIK_003298"
     CDS             complement(13255..14418)
                     /locus_tag="ACHKIK_003298"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO9084413.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(14421..15059)
                     /locus_tag="ACHKIK_003299"
     CDS             complement(14421..15059)
                     /locus_tag="ACHKIK_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO9084414.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(15069..15467)
                     /locus_tag="ACHKIK_003300"
     CDS             complement(15069..15467)
                     /locus_tag="ACHKIK_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO9084415.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(15485..16144)
                     /locus_tag="ACHKIK_003301"
     CDS             complement(15485..16144)
                     /locus_tag="ACHKIK_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO9084416.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16195..16893)
                     /locus_tag="ACHKIK_003302"
     CDS             complement(16195..16893)
                     /locus_tag="ACHKIK_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO9084417.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(16912..>17111)
                     /locus_tag="ACHKIK_003303"
     CDS             complement(16912..>17111)
                     /locus_tag="ACHKIK_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO9084418.1"
                     /translation="NTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILL
                     EITSLVDNALDLAEITEEYSH"
BASE COUNT         4354 a         4579 c         4095 g         4083 t
ORIGIN      
        1 aaacattaaa caggtaacat tacaatgaca ttaccaacga ctatttattc atttcctgca
       61 tattcacccc atttttcgtc caccgacaaa ccagtaaaat taaaatttca tcaatatgca
      121 cgtgctacat tactgccaaa tcgtggaaga gatcataatt gcgatggcag acgtactgtt
      181 gagatccaca aactagatct ctctgactgg caagcattca ataagttagc aacgaagtgt
      241 aatgcttacg atggagtaac aatgaatggt gataattctt ttggatggaa ccataaagct
      301 acattagata atattcacgc ccagaaatac aataaagctc acgcgggtgc gaggttaacc
      361 gcagaactga aatatttatt gcaagatgtc gaatccttcg aacccaacag taaatacaca
      421 atacacgaag tggttttggg acctggttat ggtactccag actataccgg ccaaacgata
      481 ggatacgttg taacccttcc tgcccaaatg cctaattgct ggtcatctga actaccaaca
      541 atcgatcttt atatcgacca acttcgcact gttacaggcg tttctaatgc cctcggattc
      601 atcattgctg cattgctaaa cgcgtattct gatttacccc atgatttgaa aattggtcta
      661 cgttccttat caaattcagc agcaatctac tcgggtttag gtttcgagcg agtgccgcag
      721 gaccgagaca taaggagtga tcgtatgcat ctaacgccag caaaccatcc tgacttatgg
      781 acacaagaga atggtgaatg gatctacttg cgaaactaat acctgacgaa aaatagaaag
      841 caaaacgccg accaatggtc ggcgttttta cgtctcgttg aataacgaat tattcagcta
      901 ctacgtttac gatcactttc gcgaatactt cgctgtgaac ctggaagctc acttcgtgtt
      961 cgccagtggt acgcagaacg ccgttcggca gacgaacttc gctcttagcc acttcaacgc
     1021 cagctgcagt tacagcgtca gcgatgtcgc gagtaccgat ggaaccgaac agtttacctt
     1081 cgtcgccagc tttagacgcg atggtaacag tttccagtgc attgattttc tcagcgcgag
     1141 cattagcagc tgccagaact tcagccagtt tagcttccag ttcagcgcga cgtgcttcga
     1201 agaattcaat gtttttcttg gtagctggaa cagctttacc ctgcggtacc aggaagttac
     1261 gagcatagcc cgctttaacg tttacctgat cacccaggct acccaggttt gctactttat
     1321 caagcagaat aacttgcatt accttatcct ctcaaagtcg tattaatgga ccgtgaccga
     1381 ttactgatgg cgatcagtgt acggcagcag ggacaggtag cgagcgcgtt tgatagcgcg
     1441 agccagctga cgctggtatt ttgcacgggt accggtgata cggcttggga caatcttacc
     1501 gctttcggtg atgtagtttt tcagcgtagc gatatcttta tagtcgatct cttgaacgcc
     1561 ttccgcggtg aaacggcaga acttgcgacg acggaaataa cgtgccatat ggctagtctc
     1621 cagaatctat caattcaatc tgctcggcat gcaaaaccat tttgctcagt ccgttctttg
     1681 ccttgtggca tgaaatgaac ccctgaacgg ttatgcgact gccgaccgtt atactgtgag
     1741 taatggcctg gttttcgtgt ccgctaacaa taacgggcat ttgacaccat gcctgccggt
     1801 gaaagccggc ttcctcctgc acagaacgat gctcaagcac gaactggcag tgaggaattc
     1861 ctgatggact gacctttcga aggggagtcc tgcacacggt gccggacaac accagacggt
     1921 tggtcatcag aaattactct tcagaatccc cagcttcagc atcatcagcg gtttcgtttg
     1981 cgaaatcatc gcgacgctca cggcgctcgt ctttcgcttt aaccatcgga gatgcttcgg
     2041 taacagcgtg cttggtacgc ataaccatgc tgcggataac ggcatcgttg aagcggaagg
     2101 tagtttccag ctcatcgatc acttcctgcg gagcttcaac gttcatcaga acgtagtgtg
     2161 ctttgtgcag tttgttgatc gggtaagcca gctgacggcg gccccagtct tccagacggt
     2221 ggatcttgcc ttctgcacca gtgatggcag cagtgtagcg ctcgatcatg cccggaacct
     2281 gttcgctctg gtcaggatgg accataaaaa cgatttcgta atgacgcatc gaattgctcc
     2341 ttacggatta ttcagcctcc tgtctgggtc agccgaatcc cggggaggca aggaacgtgt
     2401 taaaggtcgg ctgaaaaatg acgcgttata gtacttgtat gccccgctaa actcaaggcc
     2461 agccgacaaa taaatctcat ctttcgctca aaagaactaa ctccctgatt tgaagttcat
     2521 cacattccgt cacagtgttt tttcgaacaa ctgcatcaga aatggtcacc atcaacttct
     2581 ttatcattga ttaaaattaa atcagaagat catcacagag gagagcacac aaagtcagcc
     2641 gttgaggagg tatctatgtt cagtcgtgtt ttagcccttc tggctgtgct tttgctaagt
     2701 gcaaatacat gggcagccat tgaaatcaat aaccaccagg ccagaaatat ggacgatgtg
     2761 caaagcttag gcgtgattta tatcaatcat aatttcgcca ctgaaagtga agcacgtcag
     2821 gcattaaatg aagaaacaga tgcgcagggc gcaacgtact accacgtaat tctgatgcgg
     2881 gaaccgggga gtaacggcaa tatgcacgcc agcgcggata tttatcgcta gcaccaggta
     2941 taaccaacga aacattgcca tagtttgctt tgcccccttc gcagggggct ttttttgaaa
     3001 aacggctgcg gaattagacc agttatctcc cgaagaagga aatttccgca gcgcgtgttt
     3061 tacttctgcc cgtaataagc gttaggacca tgcttacgca tgaagtgttt attcatcagg
     3121 aagctgtcga tgtgattgag ttgtggatta atactgcgcg caatccacgc cattttcgcc
     3181 acttcttcca tcactaccgc gttatgcacc gcgtcgtggg catctttccc ccaggcgaac
     3241 ggtccgtgct gatacaccac aattcccggc gtatgcagcg gctcggcgtt gcccagcgtt
     3301 tcgataatca ctttgccagt gttcagttca tactcaccct gcacctcttc ttcgcttaat
     3361 ccacgcgtac acgggatgtc accaaagaag tagtcagcgt gcgtggtgcc taacgccggg
     3421 atcgccagcc ccgcctgcgc ccatgcggta gcatgagtgg agtgggtatg gacaatgccg
     3481 ccaagcgacg gataacgacg gtagagttca agatgcgtcg cggtgtcgga agatgggcga
     3541 tactcccctt ccaccacctt accgctcata tcgaccacca ccatatcgtc tgctttcatg
     3601 gtttcgtagg cgacgccgct gggcttgatc accaccagcc cgcgttcgcg gtcgatagcg
     3661 ctgacgttgc cccaggtaaa ggtcaccagc ccgtagcgcg gcagatccat gttggcttca
     3721 aatacctgct gttttagctt ttgcattatg ccgcctccac catgcccgct ttcgccatgc
     3781 gcgctttcac ccaatcacgc gctttcgcca cttctgccgc cgggtcttcc gccgtttcgc
     3841 tccacatctc aatcaggtac ggcccgcaat agccactctg tttgagcgtt tcgaaacaac
     3901 gttcgaaatc cactacacct tcgccaaacg gcacgttttt gaagacgcca ggtttggtgt
     3961 ctttcacatg taccgcgacg atatgcccga ttccggcctg caactccatc tgcacatcgt
     4021 tgtcccacgc cgacaggttg ccgatatccg ggtagagctg gaaccacggg ttgttgagat
     4081 agtgcgcgta acccagcgcc ttgctgatgg agttcatcaa cggataatcc atgatctcca
     4141 tcgccagcgt cacctgcgcg cggctcgcca tctcaacgct ctctttcagg ccgtcacgga
     4201 aacgacgacg cgtttcgtta ttggcttcct gatagtaaac gtcatagccc gccagctgga
     4261 tcacgcgaat gccgacgtcc tgggcgaact ggatagcttt acgcataatc tccagcccct
     4321 gcgcccgcac cgcgtcatct tcactgccca gcgggaaacg acgatgagca gaaaggcaca
     4381 tggacggcac gcgcacgccg gtttcaacaa tcgcattcac cagcgccaga cgctgctcgc
     4441 ggctccagtc gaggcgcgac aggcgctcgt ccgtttcatc taccgacatc tcgacaaaat
     4501 cgaagcccaa cgtttttgcc agttgcaggc gttccagcca gcactccccg gcggggagcg
     4561 ctttttcata gatgccaagc gggatttgtt tggacaacat atccgctcct tagccccaca
     4621 gttcggcgat ggaacgtttg aactgacgtg cagcttcaac cggagaagcg gcatcacgga
     4681 tgctgcgacc agcgataaac acatgaatcg gaataccttt gaacagcggc agatcttcca
     4741 gcgccaggcc accggtgacg gtgactttga agcccatatc ggaaagacgt ttgatcgcgg
     4801 taatgtccgc ttcgccccac gctacgcctg cggcctgcgc gtcacggctg cggtgataaa
     4861 ccacctgctg aatgcctgcg tcacgccact gctgcgcctg ttcccaggtc cagtagccgg
     4921 tcaattcgat ctgcacgtcg ccgttaaact cttttgctac gtccagcgcg cctttggcgg
     4981 tgttgatatc cgcacagcaa attaccgtca cccagtcagc gttggcttcg aagcacatac
     5041 gcgaaaggat tttgcccgca tcagcaattt tagcgtctgc cagtacgatt ttgtgcgggt
     5101 agagcgcttt caggtcacga accgcacgca cgccttcgcc cacgcacaga atggtaccca
     5161 cttcgataat gtcgacttct tcggcaatca ggcgagtggt ttcgtaggcg ctatccatag
     5221 tctggttgtc cagcgcgact tgcaacatcg gtaatgacat cttcaattcc ttcttaagct
     5281 gccgcgttgg tgcggtcaat gagatccagt acttcctgct cggtacggca ggcacgtaaa
     5341 cggtcgaaat tttcttcatc ttcaaacagg ttgacgatct gcatgatgcc cacttcctgg
     5401 tgcgtgttgg catcgaccgc cgccatggtg atgaggatat ccaccgggtc gttatcttcg
     5461 tggttgaact ccagcggctt tttcagcgtc accagcgcaa aaccggtttt cttaacgccc
     5521 tcttccgggc gaccgtgcgg cattgccagg cctggtgcga gcacaaagta cggaccgtgc
     5581 tgttcaaccg catccagaat cgcctggtag taacgcggct cgacaacatc cgccgccacc
     5641 agcaggtcaa cgccgatttt tactgcgtcc tgccatgtct cagcttcagc ctgcaggcgg
     5701 atggatttat tctccgccag cgaatcacgt aatttcatgg cgcgtcctta cttcacatcc
     5761 tgcgggaaat gcgctttgat cacttccagc agtttcgggc caaagtcggc aggagagagc
     5821 atgttgcgca cgccaaccac gtatttgttg ccggtcacgg tgatttcgcc cgcaatgtgc
     5881 gtagaagcga tgatgatatc cgcgccgctc aactcgcttt tgtattcgcc aaccgcgcag
     5941 ctatttaccg tgtggtcaat gtttgattgg gttaaaaact ggtccacttt catcttcatg
     6001 atcatggaac tgccttgtcc gttgccacac acagccagaa tacgtacggt cataatcaaa
     6061 actccttatt aagcagactg ttctgccagt tgtttttctg catcttcttc tgcgcgcagt
     6121 gcgcggccag cgaagaacat ataagccagt gcaattacaa tgatgacggc cataaaggcg
     6181 ataccgatgg agaagaagcc ctgcatcatc ggcggtgcca gaatcgacca gtccgccatg
     6241 cccatccagg cgctcatacc ggtgagtttc accgcccaga cgcagccaaa gatttcaatc
     6301 atccccatca ccagacatat cttcagcgcc gcacgccagc cgccgaagtg gttagcgaac
     6361 acgccgatgg tggcgttgga gaagaacatc gggataaagc cgggaataat caggatcgag
     6421 gaaccgcagg cgaccaggat gccaaccgca atcagctgac cgatggtgcc ccacataaag
     6481 ccccaaacca cggcgttcgg cgcgaagcta tagatagccg cacagtcaat cgccagaacc
     6541 gcaccaggga tcaggcgctg ggagataccg ttaaatgctt cagagagttc cgccacaaac
     6601 atgcgcacgc cctgagtgat gatgaagatc gccaccgcga aggagaaacc agtttgcagg
     6661 atgtacaccg tccagttcac tttgcctgcc atcgcctgca cggtgtcgat accgaaggag
     6721 agcagaatgg caccaaagaa gatggtcatc acaatcgccg tggagacgat gttgtcgtgg
     6781 aaaatattca gccagcctgg cagtttgagg tcttcaacgc tttcctcttt tttgccgagg
     6841 aacggcgcaa ctttataggc aatccatgat gcaaactgtt gctggtgacc gatggagaaa
     6901 ccgcagccgt ccgtcacttc ctgagtcggc ttgtacatca tgttggaggt aatgccccag
     6961 tagagcgaaa ccagaatcgc cgtgcagata atggttgtcc acatggagta gccgaaaata
     7021 aacaacgtaa cggcaatcag ccccgcctgc tggaacatga tataaccagt cagcatgatg
     7081 gtacgaatac cggtaatgcg acgcagcagc acgtaacaga tgttcagcgc cagcgctaac
     7141 aacaccgcgt aacctaccca gctataggca tcgcccatgc ggtcgatggt tgccatcatc
     7201 gacgcgtaag tatcggaaat tgcgccgtta atgccgtaga cttcggacat tttcgccacc
     7261 accggtttga aggtgctggt gaggatgccg gaccctgcct gcaacaacat gaaaccaatt
     7321 atggttttaa tcgtgccttt aataataacg ctgacacttt tgcgcagtag gatgtagccc
     7381 agacaggtca caatacccag caacaacggg gcattggtca tgacctggtt aaaaaacacg
     7441 gtaaagatgt tgtagaggat ctccataacg atctcctgaa gaagaggtaa ccgggtattc
     7501 cgcacacccg gaatgttgtg cactcactct aattttcaaa agtaatcaca acaagattat
     7561 ttatgattaa atgtgacgcg acccgcaaat aattccacac ggatagagtg ggattcacac
     7621 aacgaaacaa ttatttattt aaattcattt agttaattta actacacatt ccattaaatc
     7681 tttccacact caaaatcaac aacaacttcc ctggcaaaaa gtcaaaaaat ggtcattgcc
     7741 ttcctgaaaa aatagtgtca ggattaatca aaaccaatca catattgatt cgaattgaac
     7801 tatcatgaag gtaaatggcg atgagtaaag tgaaaagtat cacccgagaa tcctggatcc
     7861 tgagcacttt cccggagtgg ggtagctggc tgaatgaaga aattgaacaa gagcaggtcg
     7921 cccctggcac atttgcgatg tggtggctag gctgcaccgg gatctggttg aaatcagaag
     7981 gtggcaccaa cgtttgcgtt gatttctggt gcggcactgg caaacaaagt cacggtaacc
     8041 cgttaatgaa acagggtcac cagatgcagc gcatggctgg cgtgaaaaaa ctgcagccaa
     8101 acctacgcac caccccgttt gttcttgatc cgtttgcgat tcgccagatc gacgcggtac
     8161 tggcgactca cgatcacaac gatcatatcg acgttaacgt cgctgctgcc gtgatgcaga
     8221 actgtgctga tgacgtacca ttcattggac cgaaaacctg cgttgatttg tggattggct
     8281 ggggcgtacc gaaagagcgt tgcatcgtgg tcaaaccggg cgatgttgta aaagtaaaag
     8341 acattgaaat tcatgcgctt gacgctttcg accgtactgc actgatcacc ctgcctgccg
     8401 atcaaaaagc ggctggcgta ctgccggacg gcatggacga tcgcgcggtg aactatctgt
     8461 tcaaaacgcc tggcggctcc ctgtatcaca gcggcgactc ccactactct aactattatg
     8521 cgaagcacgg taacgaacat cagatcgatg tggcgttagg atcgtatggt gagaacccgc
     8581 gcggtattac cgacaaaatg accagcgccg atatgctgcg tatgggtgaa gccctgaatg
     8641 cgaaagtggt tatcccgttc caccacgaca tctggtcaaa cttccaggcc gatccgcaag
     8701 agatccgcgt gctgtgggag atgaaaaaag atcgtctgaa gtatggcttc aagccgttta
     8761 tctggcaggt tggcggcaaa tttacctggc cgctggataa agacaacttc gagtaccact
     8821 atccgcgcgg tttcgatgat tgcttcacta ttgaaccgga tctgccgttc aagtcattcc
     8881 tgtaatctgt cgtttatacc ggatagcgtg gagaagttcg cgctatccgg cgtagtattt
     8941 cctggctatt tcaaatatca tctaaaaaaa tcaaatttta tcggaatagc tcatgactga
     9001 agcacaaaga catcaaatcc tcctggaaat gctcgcacaa ttgggctttg tgaccgttga
     9061 gaaagtcgtt gagcgtctgg gaatttcgcc tgccactgcg cgacgcgata tcaataaact
     9121 tgacgaaagc ggcaaactga aaaaagtgcg caatggcgca gaagctatta cccagcagcg
     9181 cccgcgctgg acgccgatga atctgcatca ggcgcagaat cacgatgaaa aagtacgtat
     9241 cgctaaagca gcctcgcagc tggttaatcc gggcgaaagc gtagtcatca actgcggctc
     9301 caccgcgttt ctgcttgggc gggaaatgtg tggcaagcca gtgcaaatca tcactaatta
     9361 tctaccgctg gcaaattacc tgatcgatca agaacatgac agcgtgatca ttatgggtgg
     9421 acagtacaac aaaagtcagt ccatcacttt aagcccgcag ggcagcgaaa acagtctcta
     9481 tgccgggcac tggatgttta ccagcggaaa agggctgacc gcagaagggt tgtataaaac
     9541 cgatatgctg acagcaatgg cagagcagaa gatgctgagc gtggtaggga aactggtggt
     9601 actggttgat agcagtaaga ttggcgaacg cgcgggaatg ctttttagcc gtgccgatca
     9661 aatcgatatg ctgatcaccg gcaaaaatgc taacccggaa atcctgcaac aactggaagc
     9721 gcaaggggtc agcattctgc gtgtttaaag atgctggcgg aaaaatgtca cggcagcatc
     9781 taacgcctca ggcgtaatgc ggtgacgcac gcctggctgc catgaacagg ttagcagttt
     9841 atcccgtccc gtctcgctta aggcctgctg caaacgtagt gattcgtcgg caggcacaac
     9901 gtcatcgtcg aggccatgcc acagcagtag aggtctgtcc ccaagttgtt ccaggtggtt
     9961 tgtcgcttcc cactctgcca gcggcgcgat aatgttattg aattcattct gctgtgctgc
    10021 cgtttcaggt atcagcggtg gaaacagtga acgggcgagc gatgtaaaat agcccgatcc
    10081 catcatgctg gcggtacatt tcaccgtggg gtggcgagtg gtaattccca gtgccgtcat
    10141 cgcgcccatc gacgcgccac cgactgccag acggtcatca agcagccagt tttcttcggc
    10201 tattgccgca cgtaaagtag tgaattcctg catactttgt agcaagattt gccagaattg
    10261 atttaaccgc cgcgcttcgt cgctactaaa acggctaccg tgatcgggcg catccggcat
    10321 gatcacccgc aaaccagcct gcgccagcgc aacggcaaaa tagctataca ccaggctgga
    10381 tgaagtaaag ccgtgataaa aaattacgca cggtaacggg gtatcttttt gcccgacagg
    10441 ataagcatga agaacgggaa tatctgccag ctcgcgtgat tctatttcaa tcatctgtct
    10501 ctcctttttg tctggtatga catgaagaaa tccttagcac aagtttctgc tggcggtatt
    10561 agttcataat gttgagatgt gggttacgct ttcgtcacgt tttcattcga aaatcgcgta
    10621 ccaggtaaca attcgggaac attcccccaa agttaaatta ataaggcact acactatggt
    10681 tagcaggaaa cgtaatagcg ttatttaccg gtttgccagt ttattattgg tgttgatgtt
    10741 aagtgcctgt agcgctctgc aaggtacgcc acagccagca ccgccagtaa cgaaccatcc
    10801 gcaagagatt cgccgcgacc agacgcaagg gttacagcga ataggtagcg taagcacgat
    10861 ggttcggggt tctccggatg acgcattagc agaaatcaga gcaaaagctg tcgctgcaaa
    10921 agctgattat tacgttgtcg tcatggtgga cgaaaccatc gtgacaggac agtggtattc
    10981 acaagccatt ttgtaccgta aataaatcag acaacttaca tagtcagctt tacattgctt
    11041 tgcgtcccta tgcgtttacc tgtgcacaat aaattaccgg ctggaagcct gggcgtttat
    11101 taccgcgatg gaatgccctg caacatgtgg ggaaacgaaa aatggagctg acgatgaaac
    11161 aattacttgc ctcaccctcg ctgcaattag tgacttatcc tgcgagcgcc acggcacagt
    11221 ctgcagaatt cgccagtgct gactgcgtga cgggcctgaa tgaaataggt cagatctcag
    11281 ttagcaatat ttcaggggat ccgcaggacg tggaacgtat cgtggcgtta aaggccgatg
    11341 aacaaggtgc gtcatggtac cgcattatta ccatgtacga agaccagcag cctgataact
    11401 ggcgcgtaca ggcgatactg tatgcatgag tattatatac agcaaatgaa caatgccgga
    11461 tgcggcgcga gcgccttgtc cggcctacaa tgttcgcata tttcattgag ttaatcgaaa
    11521 ccagcgtcgc atcagtcgta tacgcttaca cacacactcc ccccgtcgcc cgcagcaata
    11581 aatcattctg gatctgctct gacaaccgta cgccgccgcg cgtatctaac atcacctgac
    11641 accacgcctg cgccattggc ggagaggcat atttcaacat ttgcgcaccg cagcccagca
    11701 ggaatagctg atgagtaatc tctcgcccca gttcttcagc tggcttgcgc agctgctgct
    11761 gtaaacgacg aaccgcgcga tcaaaatagc gatcctgccc tttcacttcc acaaacgctt
    11821 ccgacaataa gtcgtatacg cccgcttgct tattgagaac gcgcagcaca tccaggcaca
    11881 taatattgcc ggaaccttcc caaatactgt ttaccggcat ctcccggtaa agccgcggca
    11941 gctcgctctc ctcgcaataa ccaatgccgc ccagcacctc catcgcttcg gccacaaacg
    12001 gcatacctct tttgcagatc acaaattttg ccgcaggcgt aaataaacgc gcccacaggg
    12061 cttctttggc atcggcacgc cggtcccacg ctcgcgcaag acgaaacagc aacgccgttt
    12121 gcccttcaag ctgaagtgcc atgcgactta agacatgacg cataaggggc tgttggatca
    12181 atggattacc aaaaacatgg cgttgatgtg catgataaat cgccagcgaa aatgcacggc
    12241 gcatcatggc atggctaccc agggcgcaat caaaacgcgt catcccaccc attttcagga
    12301 tcagacgaat tccttcccct tcctgcccca acaaccagcc aatggcatcc tgaaactcca
    12361 cttcgcagct ggcgttagag cgattaccca gcttatcttt cagccgctcg aggcgaatcg
    12421 cgttgcgttg cccgtcaggc aaaaagcgcg gcacaaaaaa gcaggacaaa ccgcccgtag
    12481 tctgcgccag caccagatgc gcatcgcttt gcggcaccga gaaaaaccat ttatgcccca
    12541 ccagccgata agagccatct tccaggcgct ctgcgcgggt ggtgttgctc atgacatcgg
    12601 aaccgccctg cttttccgtc attcccatgc caatcaacaa accgcgtttt tgcccacctg
    12661 gcaataagtg agaatcgtag cgatcgctca gcagcggcgt ggtccagtcc tgaaacggcg
    12721 tgggtaacat ctgtaacaac aatggcgtgg cggcaaaggt catcgttatt ggacataacg
    12781 acccagcctc aacctgcgca tgtaacataa aacgcgccgc gcgcgccaca aatgcgccgg
    12841 agcgagcgtc ttcttcccag gcaagattgt gcacccgatt ggtacataac gcctgcatca
    12901 gcaggtgcca ggcggggtga aaacgcacat cgtccagacg gcgtccttgc gcatcgtagc
    12961 gcaataattc aggcggattc acattcgcca gccgccccag ttcaagagat tcagccgttc
    13021 ctaactgctg accaatactg gctagaaaat cgctatccca gccagcacct tcacgcgtta
    13081 ccgcttcaca gagcgcgcca tcagacaggt ataagttgct gttatttaat ggtataggtt
    13141 gattaaaaac ggtgtgagtt tgccagtgca ctgtgtctcc ctccatcaat ggcagtcacc
    13201 cttagtatgg tcactgccat ggattcctga cagaaagaga ttgctaaaac attcttatcc
    13261 agcaatgtaa tgcggaacga aacgtgaggt gtctttggtg atcagcgtgt tatcttcacg
    13321 gatccccatt ccgcacgctt catcatcgac aatccagcta ccgatgagtg tgtagctatc
    13381 gccaaatcgc ggcagaggtt gaaacgcctg gtagatcatc ggttcatcgg cgtaatcacc
    13441 atcagcgtgg tcaacgacgt tattctgacc gtcgaaaatg gtgacgtttc cgccttcgcg
    13501 cgagtagatc ggtttacgca catagctttc gccagcggca atctgcggtt tttcgccctc
    13561 aaaccaggat gcaagaaggt taggatgacc agggaagaag cgccaaagca gcggcattag
    13621 ccctttatta ctcaagatac ttttccataa cggctccacc cattgctcgc gacgcttgcg
    13681 cagcagcgga ccgttatcgt cacgcatcat ccactccagc ggatacagct taaatgcacg
    13741 ctggatgaca ttatcatcaa gatcggtcag tacaccgccg acgcccaaac cgagatcttc
    13801 aatgtagata aaccgcgact cctgccctgc ctgctgggcg cagtcctgca agtacagcac
    13861 ggtagtcctg tcttcatcgg tgtcctgaca gcagcagaaa taaaacggct cccggctata
    13921 aagctcgcta aagcgtgaaa tcaggcgttc ctgaatggca ttgtactgat cggcatcacg
    13981 cggaataatg ccgctgcgcc gggcatcctc cagccacaac cactggaaat aagccgactc
    14041 atacaacgag gtcggcgtat cggcgttgta ctccagcagc ttcaccggcg cattgccaca
    14101 ccaggcaaaa tccatccggc catacagcga aggatcgcgt gcgcgccagc tttcggcgat
    14161 cacatcccag tacaacggcg ggatcgccag ttgcgtcagg atctcttcgt ctttaaccgc
    14221 gcgatcaacc acctcaaggc acatctgatg cagttccgca gtcggttttt cgatctgctc
    14281 ttcaatctgg cgcagagtaa aacggtaagc ccgactctcg tcccaataga tttcattgtc
    14341 gatgatatga aagtcgaaac cgttgttagc ggcgatctgg tccagatccc gtcgcacagg
    14401 aacgttgtgt ctcagcatga ttagcctccc caatgcccac gggcgctgga agaacgacca
    14461 tagccgccgc gcgaaacggt tgacgctttt ttggtggtaa agccgcctga agagtaagac
    14521 tcttttttgc cagaaccgga gcgccaggag taatcaccgg aagtgttgcg ccagacgggg
    14581 cgggaagcaa atgaggatcc gccgctgtta tagacgaacg gctcatcgcg atctttacga
    14641 ataacccggc ttaacaaaaa tccagaaacg accgggatcc aactctgttc aacattgtcg
    14701 tagtagcaat tttcgtactt agactgacaa ttctgctgag tcatattctt cggaacatcg
    14761 gcataaaatg ccgctttggc gttgttccag ccacgcgcgc aaatgtcggc attattaccg
    14821 tcatcaatac aatcctgcac tgtcgaataa aacgttccgt cgccgtcgtt atcgacatcg
    14881 ccactatcgc cacaaccttt caatacaaaa aaagcggcac cgcccattag agccaacgtt
    14941 aagtattttt gtgcgtagcg attgcgacgc ggttcaaaag gattattcgg tctaccaatg
    15001 cgactgatcg ctccgtgacc aattttactg ttatttcttg atttgcgttt tctggccatg
    15061 atcgtccatt accatgtcat acaagctgcg ttaaaaatac cgccagccag cgcggcggtt
    15121 cccataaaca ttcctgctgc ggtattgtga ttaataattt tttcgcttaa tgcgggcata
    15181 taaagtctga caccagcaaa aacgagtaac tgaatcacca gtgcaatccc gccccaggcg
    15241 aaatagtctg gaatactcac cgcattgatt gccgcactgg ataatgggat aacgtaacct
    15301 aacaatgtac cactgaacgc cagtgaagcc gcggtattat tgtttttgat taactgccat
    15361 tcgttgtgcg gtgtaatttt agaatagata aaaagaaaaa taatcaccat agccacgcca
    15421 ataaaaaaat aggcgctaaa ggcaagaagt gaatccagta tatgcattgt aaacgtcctt
    15481 gtaattaacc aataatatga agtgatgtca gcgggatatc gacacccaac gcgcgcgaaa
    15541 ataaccactc tggctcgccg agatcgttaa aagattcctc accatttaac agcaaatatt
    15601 cgtaagtatc ttcggttact tgccgctggt agcccatggt aaaattatgg acctcccatt
    15661 tggcatggtt ttgattttct actttttcca gcatgtagac gggttcgata tttcccggtt
    15721 cttcgctgtt aaaaaatcgc tgccagcgtt tttcctgcca gtttaagatc attgccccca
    15781 tcactttggc gttgatcgcc tcgcgccagt gactctcttt actgataccg tagctctctt
    15841 catagacaaa gagcttgata tcatcaatgt catcaatatc ttcgccgccg gtggtattga
    15901 tttgcagaaa ttcatcgcct gaagtgtagt agcggaaaat ctgactaccg ccgcccagat
    15961 cgatgtggct gacagcggct accgtaaatt cctcacccgg cagcgcaatc agcaattcat
    16021 cttccagcaa acgaaacgct aacgtgtcga gcgtaaagcc actattgaga tgaagtccca
    16081 gcggaccgcg agcgatagca ggcttattat ccttgccaaa cagacgctgg aaaaaaccag
    16141 acatatttta tccttaaaat agttgccagc cttttagcga ggctggcaaa aaaattactc
    16201 gccctgttgg cgttgcagtc ttgctaatac atcctgggcg ctacttttat tgctaccgcc
    16261 aattccggct tccgccagct tttcatcaag atcgcgaccg tctgcgactt tttccaactg
    16321 tgcagcagca tccaggcgag cctgacgttc ggcctgacgc gtttgcaggc gttttaagga
    16381 ttctgccgct gtcgaaacgc tggaagatgc gccaacggta gaggttgtta cagcctgttg
    16441 tgcacgctgc atggcttcag tggctttaac gacttccatt tgctgctcaa actgagcgat
    16501 acgctgcgct gtcgctgtaa ctgctttttc cacgccatca cgggagactt ccagattcga
    16561 caacacttgc tcttcagcgg taatgagatt ctcaaggcgc gcgatttctt cagcaacttc
    16621 gttaatcaac gacggattaa cgttcttgct taacgcttcc agcgcacggg cttccagact
    16681 ggctttgcgc tcacgtaaat ctttcagctt atcgtgactt aatttcaccc gcgccagcag
    16741 atcaacgcga gattttccgg ctttatcgag ttcagcttta gcgtcacgaa tatgctgttc
    16801 cagcatgcgc acgccctggg tttcttcaat ggattcttcc gcctgggaga taaacgattt
    16861 ccccagcgta aataaacttt ttaaaattcc cataaatacc cgtccttgta attagtgtga
    16921 atattcttct gtaatttcag ccagatccaa tgcgttatct accagcgagg ttatctcaag
    16981 caggatatct tcaagagaag atttaagcga taacgccccg aaaacaatgt aatactcttc
    17041 ctgttgcacg ctggagattc ctaccgatga caacggcatc atcttctgat ttcttaataa
    17101 gaaggtatta a
//
LOCUS       DAZGJW010000098        16909 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZGJW010000098 DAZGJW010000000
VERSION     DAZGJW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.098"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..461
                     /locus_tag="ACHKIK_003304"
     CDS             <1..461
                     /locus_tag="ACHKIK_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO9084419.1"
                     /translation="ILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKT
                     EAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRG
                     QAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDSVVSVARAVWNWIFF"
     gene            830..3676
                     /locus_tag="ACHKIK_003305"
     CDS             830..3676
                     /locus_tag="ACHKIK_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HFO9084420.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNGGTLTNHDNQIVFGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATVTVINEKGWQAVKSGAMATDTVVNTGAEGGPDAENGDTGQNVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATDVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIHFDNQTTQDAVLSRAVAKGDSPVTFHK
                     LTTTNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATTGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGGYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     VHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAK
                     HSVRELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            4004..4822
                     /locus_tag="ACHKIK_003306"
     CDS             4004..4822
                     /locus_tag="ACHKIK_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO9084421.1"
                     /translation="MRLASRFGHVNQIRRDRPLTHEELMHYVPSIFGEDRHTSRSERY
                     AYIPTITVLESLQREGFQPFFAYQIRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPSRQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRTIHSIDTDIKLNRALWVMAETLLESMR"
     gene            4914..5399
                     /locus_tag="ACHKIK_003307"
     CDS             4914..5399
                     /locus_tag="ACHKIK_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9084422.1"
                     /translation="MTTVTHNSTTPSVSVTAASGNNPPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRMENYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            5415..5891
                     /locus_tag="ACHKIK_003308"
     CDS             5415..5891
                     /locus_tag="ACHKIK_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO9084423.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            5954..6175
                     /locus_tag="ACHKIK_003309"
     CDS             5954..6175
                     /locus_tag="ACHKIK_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO9084424.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            6249..6656
                     /locus_tag="ACHKIK_003310"
     CDS             6249..6656
                     /locus_tag="ACHKIK_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO9084425.1"
                     /translation="MSDTLHGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTITLYAKGLTCEAD
                     TLGSCSFVYMAVYATRFSNQLPKASVHQLTHYF"
     gene            complement(7197..8444)
                     /locus_tag="ACHKIK_003311"
     CDS             complement(7197..8444)
                     /locus_tag="ACHKIK_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO9084426.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(8516..9430)
                     /locus_tag="ACHKIK_003312"
     CDS             complement(8516..9430)
                     /locus_tag="ACHKIK_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO9084427.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            9646..11079
                     /gene="cscA"
                     /locus_tag="ACHKIK_003313"
     CDS             9646..11079
                     /gene="cscA"
                     /locus_tag="ACHKIK_003313"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO9084428.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(11087..12082)
                     /locus_tag="ACHKIK_003314"
     CDS             complement(11087..12082)
                     /locus_tag="ACHKIK_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9084429.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            12325..12543
                     /locus_tag="ACHKIK_003315"
                     /pseudo
     CDS             12325..12543
                     /locus_tag="ACHKIK_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            12561..13889
                     /gene="dsdA"
                     /locus_tag="ACHKIK_003316"
     CDS             12561..13889
                     /gene="dsdA"
                     /locus_tag="ACHKIK_003316"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO9084430.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKMTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(13997..15535)
                     /gene="emrY"
                     /locus_tag="ACHKIK_003317"
     CDS             complement(13997..15535)
                     /gene="emrY"
                     /locus_tag="ACHKIK_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO9084431.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(15535..16698)
                     /gene="emrK"
                     /locus_tag="ACHKIK_003318"
     CDS             complement(15535..16698)
                     /gene="emrK"
                     /locus_tag="ACHKIK_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO9084432.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         4447 a         4288 c         4159 g         4015 t
ORIGIN      
        1 acatcctgca ccggggacat cagcaggtgc tgtttgtggt gacgcaggcc gacaaaacgg
       61 agccctgcca tgaatgggat atggccggta ttcagccttc tcccgcacag gcacagaata
      121 ttcgcgaaaa aacggaggcg gtattccgtc tgttccggcc cgtacatccg gttgtggccg
      181 tatcggcccg caccggctgg gaactggata cgctggtcag tgcgctcatg acagcgcttc
      241 ccgaccatgc cgccagtccc ctgatgaccc gcctgcagga cgagctgcgc acggaatctg
      301 tccggggtca ggcccgtgaa cagtttaccg gtgcggtgga ccggatattt gacacggcgg
      361 agagcgtctg tattgcctct gttgcacgca cggttctgcg ggccgtccgt gactcggtgg
      421 tctctgttgc ccgcgcggta tggaactgga ttttcttctg aacactctgc caggggaaat
      481 gcctgattac attgcgttaa ccaactgaaa acatgccccc ccctcatacc cacctctttc
      541 tcttgcgtga cttccctgct gttaatagaa taaaacgatc gataaaacag gtatcgatcg
      601 ttactatcga tcgtttatag tggttaatgg tctgcgaaaa ggcagctgaa tctcttcatc
      661 atgcagaacg gaattgcaca caacggactg atacttctct ggcggatgag agaggggaaa
      721 catttatgtc cgggaaaaac cactttatct gaccatgctg ttttgtacct gccggtatcc
      781 acttttgtgg gtaccggctt ttttattcac cctctgtaag gaaaagctga tgaaacgaca
      841 tctgaacacc agctacaggc tggtatggaa tcacattacg ggcaccctgg tggtggcctc
      901 cgaactggcc cgctcacggg gaaaacgcgc cggtgtggcg gttgcgctgt ctcttgctgc
      961 tgtcacatca gtcccggcac tggctgctga cacggttgta caggcgggag aaaccgtgaa
     1021 cggcggaaca ctgacaaatc atgacaacca gattgtcttc ggtacggcca acggaatgac
     1081 catcagtacc ggtctggagt atgggccgga taacgaggcc aataccggcg gacaatggat
     1141 acaaaatggc ggtatcgcca acaacactac tgtcaccggt ggtggtcttc agagagtgaa
     1201 tgccggagga agcgtttcag acacggttat cagtgccgga ggcggacaga gccttcaggg
     1261 gcaggcagtg aacaccactc tgaacggcgg tgagcagtgg gtacatgaag gcgggattgc
     1321 aacggttacc gtcattaatg agaagggctg gcaggccgtc aaatccggcg caatggcaac
     1381 cgacacggtt gtgaataccg gcgcggaagg gggaccggat gcagaaaatg gtgataccgg
     1441 gcagaacgtc tacggagatg ccgtacgcac caccatcaat aaaaatggtc gtcagattgt
     1501 ggctgctgaa ggaacggcaa ataccactgt ggtttatgcc ggcggcgacc agactgtaca
     1561 tggtcacgca ctggatacca cgctgaatgg ggggtaccag tatgtgcaca acggcggtac
     1621 ggcgtctggc actgttgtaa acagtgacgg ctggcagatt atcaaggaag gtggtctggc
     1681 ggatttcacc accgttaacc agaaaggcaa actgcaggtg aacgccggtg gtaccgccac
     1741 acatgtcacc ctgaaacagg gtggagcact ggtcaccagt actgcggcaa cggttcttgg
     1801 cagcaaccgt ctgggcaatt tcacggtgga aaacggtaag gctgacggcg ttgttctgga
     1861 gtccggcggt cgcctggatg tactggaagg ccattcagcg cagaaaacac gggtggatga
     1921 cggcggtacc ctggcagtgt ctgccggtgg taaggcaaca gatgtcacca tgacatccgg
     1981 tggtgccctg atagcagaca gtggtgccac tgttgagggg accaatgcca gcggtaagtt
     2041 cagtattgat ggcatatccg gtcaggccag cggcctgcta ctggaaaatg gcggcagctt
     2101 tacggttaat gccgggggac aggcaggcaa caccactgtc ggacatcgtg gaacactgac
     2161 gctggctgcc gggggaagtc tgagtggcag aacacagctc agtaaaggcg ccagtatggt
     2221 actgaatggt gatgtggtca gtaccggcga tattgttaac gcaggagaga ttcactttga
     2281 taatcagacg acacaggatg ccgtgctgag ccgtgctgtt gcaaaaggcg actccccggt
     2341 aacgttccat aaactgacca ccaccaacct cactggtcag ggcggcacca tcaatatgcg
     2401 tgttcgcctt gatggcagta atacctcaga ccagctggtg attaatggtg gtcaggcaac
     2461 cggcaaaacc tggcttgcgt tcacaaatgt cggaaacagt aacctcgggg tggcaaccac
     2521 cggacagggt atccgggttg tggatgcaca gaatggtgcc accacagaag aaggtgcgtt
     2581 tgccctgagc cgcccgcttc aggccggcgc ctttaactac accctgaacc gtgacagcga
     2641 tgaagactgg tacctgcgca gtgaaaatgc ttatcgtgct gaagtccccc tgtatgcctc
     2701 catgctgaca caggcaatgg actatgaccg gattctggct ggctcccgca gccatcagac
     2761 cggtgtaaac ggtgaaaata acagcgtccg tctcagcatt cagggcggtc atctcgggca
     2821 cgataacaac ggcggtattg cccgtggggc cacgccggaa agcagcggca gctatggctt
     2881 cgtccgtctg gagggtgacc tgctcagaac agaggttgcc ggtatgtctc tgacgacagg
     2941 ggggtatggt gctgcaggcc attcttccgt tgatgttaag gatgatgacg gctcccgcgc
     3001 cggcacggtc cgggatgatg ccggcagtct gggcggatac ctgaatctgg tacacacatc
     3061 ctccggcctg tgggctgaca ttgtggcaca gggaacccgc cacagcatga aagcatcatc
     3121 ggacaataac gacttccgcg cccggggctg gggctggctg ggctcactgg aaaccggtct
     3181 gcccttcagt atcactgaca atctgatgct ggagccacaa ctgcagtaca cctggcaggg
     3241 actctccctg gatgacggcc aggataacgc cggttatgtg aagttcgggc atggcagtgc
     3301 acaacatgtg cgtgccggct tccgtctggg cagccacaac gatatgaact ttggtaaagg
     3361 cacctcatcc cgtgacaccc tgcgcgacag tgccaaacac agtgtgcgtg aattgccggt
     3421 gaactggtgg gtacagcctt ctgttatccg caccttcagc tcccggggag acatgagcat
     3481 gggtacagcc gcagccggca gtaacatgac gttctcaccg tcccggaatg gcacgtcact
     3541 ggacctgcag gccggactgg aagcccgtgt ccgggaaaat atcaccctgg gcgttcaggc
     3601 cggttatgcc cacagtgtca gcggcagcag cgctgaaggt tataacggtc aggccacact
     3661 gaacgtgact ttctgataat caggtcactt cccttcaggc cccggtcatt atgaccgggg
     3721 ccttttactt tcagggatca acagatatgc cgggttgtgc atcacgttta ttgcccgaag
     3781 acccttttac tgccaaatgg catctgaatt gatcacagtc tggccagtta ttacccgatc
     3841 ccgatgggga tattgggtga tctccttcca tgaaaatacg cagctcacca gtggagggct
     3901 tctcagacaa taaacgtcat tcatttcatc tgtcaggccg cgtcttctcc gggagacgcg
     3961 gctttttcat ttataccgct aactcattca taaggagcaa ataatgcgat tagcaagtcg
     4021 ttttggtcat gtaaaccaga tacgccgtga ccgcccgctg acacatgaag aactgatgca
     4081 ctatgtgccc agtatttttg gggaagaccg gcatacctcc cgcagtgaac gctatgcgta
     4141 cattcccacc atcaccgtac tagaaagcct gcagcgggag ggctttcagc ccttcttcgc
     4201 ctaccagatc cgcgtgcgcg acccgggccg ccggggatac accaaacaca tgctgcgtct
     4261 gcgccgtgac ggggagataa acggacaaca tgtccctgaa atcatcctgc tgaactccca
     4321 tgacggtact tccagctacc agatgctgcc gggttacttc aggttcgtct gccagaacgg
     4381 gtgcgtctgt ggccagtctc tgggggaagt gcgtgttcca caccggggaa atgtggtgga
     4441 gaaagttatc gaaggggctt acgaggtggt gggcgtgttt gaccggatag aggagaagcg
     4501 tgatgccatg cagtcgctgg tcctgccgcc accgtcacgc caggcgctgg cacaggcggc
     4561 actgacttac cgttatggtg acgaacatca gcccgtcacc accgccgaca ttctgacgcc
     4621 acgacgccgg gaggattacg gtaaggacct gtggagtgct tatcagacca tccaggagaa
     4681 tatgctgaaa ggcgggattt ccggtcgcag tgccagagga aaacgtatcc atacccgtac
     4741 cattcacagc atcgacaccg acattaagct caaccgggcg ttgtgggtga tggcagaaac
     4801 actgctggag agcatgcgct gatgcctttt ccctgaaagc gcagtcctga ctcacttccg
     4861 tccctgcctc cggacattct tccattcatt tactttttat aaggaataat ctcatgacaa
     4921 ccgttacgca taattccacc acaccttctg tttccgtaac cgctgcatca gggaataacc
     4981 cacctcagct ggttgctact ctcgtccctg atgaacagcg catcagcttc tggccgcagc
     5041 attttggcct cattccacag tgggtgaccc tggagccccg tgtcttcggc tggatggacc
     5101 gtctgtgcga agactactgc ggtggtatct ggaatctgta caccctgaac aacggcgggg
     5161 catttatggc acccgaaccg gatgacgatg atgacgaaac atgggtactg ttcaatgcca
     5221 tgaacggtaa ccgcgctgaa atgagcccgg aagccgccgg tattgccgcc tgtctgatga
     5281 cgtacagtca tcatgcctgt cgtatggaga attatgccat gacggtccat tattaccggt
     5341 tgcgggatta cgccctgcag catccggaat gcagcgccat tatgcgcatc atcgactgaa
     5401 tggagagaag cacaatgcaa caactttcct ttctgcccgg agagatgacg cccggcgagc
     5461 gcagcctcat tcaacgggcc ctgaaaaccc tggaccgcca tcttcatgaa cccggcgtgg
     5521 ccttcacctc cacccgtgca gcacgggaat ggctgattct gaacatggcg ggactggagc
     5581 gtgaagaatt ccgggtgctg tatctgaaca accagaatca gctgattgcc ggtgaaaccc
     5641 tcttcaccgg caccatcaac cgcacggaag tccatccccg ggaagtgatt aaacgcgccc
     5701 tgtaccacaa tgccgctgcc gtggtactgg cacacaatca cccgtccggt gaagtcacac
     5761 ccagtaaggc agaccggctt atcaccgaac gtctggtaca ggcactgggc ctggtggata
     5821 tccgggtgcc ggaccatctg atagtcggtg gcaaccaggt tttctccttt gccgaacatg
     5881 gtctgcttta acccgtcaca accacatcac acctgttttc acttttatct tctgtcttca
     5941 gaggtatccc attatgaaaa ttatcacccg tggtgaagcc atgcgtattc accaacaaca
     6001 tccgacatcc cgtctttttc cgttctgtac cggtaaatac cgctggcacg gcagtgctga
     6061 agcgtatacc ggtcgtgaag tgcaggatat tcccggtgtg ctggccgtgt ttgctgaacg
     6121 ccgtaaggac agttttggtc cgtatgtccg gctgatgagc gtcaccctga actgagtggg
     6181 aattctgatg agcagaatta tcgccactac cggaccattc ttagccgatt ttctgtaagg
     6241 attttatcgt gtcagacaca ctccacggga caacacttcc cgacgacaat cacgaccgcc
     6301 cctggtgggg gctgccctgc accgtgacgc cctgtttcgg ggcacgtctg gtgcaggagg
     6361 gtaaccggtt gcattacctt gccgaccgcg ccggtatcag aggccggttc agcgatgcgg
     6421 atgcatacca tctggaccag gcctttccac tgctgatgaa acaactggaa ctcatgctca
     6481 ccagcggtga actgaatccc cgccatcagc ataccatcac gctgtatgca aaagggctga
     6541 cctgtgaagc tgatactctc ggcagttgta gtttcgttta tatggctgtt tatgctaccc
     6601 gattctcaaa tcaattaccg aaagcttctg ttcatcaact aactcattac ttttaatgat
     6661 ggggcccttt ctggggccca agaaattaac aacgctaaca aacaacataa ataacaataa
     6721 attaaataaa aaatttcatt cctgcagggg acaccagata cccttcaaac gaaatctacc
     6781 ttcaccccgt aaaagatagg tttggcagca cacttgcctt atatctactc atttttactg
     6841 caacaggttg aaatctcagc actgtcagaa agcgctgatg actaaacagc cctgggccgg
     6901 gcgatgtaac catcacacag aatcctgata gcgaaatatg gcgtgactcg atacttcact
     6961 ccgcaatgca ttccttgatg aattcgcagg accgtgatac acgggacagg tcgctgaatg
     7021 acgacaatgt cctggaaatc agcgaaccgc gcatctgaag tacatttgag cgactgtacc
     7081 agaacatgaa tgaggcgttt ggattaggtg attattagca gggctaagca ctttagtatt
     7141 attattttcc ggttgaggga tagggagata tcgacaacaa ccggaaaagt ttacgtctat
     7201 attgctgaag gtacaggcgt ttccataact atttgctcgc gttttttact caggaagaaa
     7261 atgccaaata gcaacatcag gcagacaata cccgaaattg cgaagaaaac tgtctggtag
     7321 cctgcgtggt caaagagtat cccagtcggc gttgaaagca gcacaatccc aagcgaactg
     7381 gcaatttgaa aaccaatcag aaagatcgtc gacgacaggc gcttatcaaa atttgccacg
     7441 ctgtatttga agacggatat gacacaaagt ggaacctcaa tagcatgtaa cagcttcact
     7501 aatgaaataa tccaggggtt aacgaacagc gcgcaggaaa ggatacgcaa cgccataatc
     7561 acaacaccga taagtaatgc attttttggc cctacccgat tcacaaagaa aggaataatc
     7621 gccatgcata gcgcttcgag taccacctgg aatgagttga gataaccata caggcgcgtt
     7681 cctacatcgt gtgattcgaa taaacctgca taaaagacag gaaaaagttg ttgatcaaaa
     7741 atgttataga aagaccacgt ccccacaata aatatgacga aaacccagaa gtttcgatcc
     7801 ttgaaaactg cgataaaatc ctcttttttt acccctcccg catccgccgc tacgcactgg
     7861 tgatccttat ctttaaaaca catgttgatc atcataaata cagcgccaaa tagcgagacc
     7921 aaccagaagt tgatatgggg actgatacta aaaaatatgc cggcaaagaa cgcgccaata
     7981 gcatagccaa aagatcccca ggcgcgcgct gttccatatt cgaaatgaaa atttcgcgcc
     8041 attttttcgg tgaagctgtc aagcaaaccg catcccgcca gataccccag gccaaaaaag
     8101 agcgccccca gaattagacc tacagaaaaa ttgctttgca gtaacggttc ataaacgtaa
     8161 atcataaacg gtccggtcaa gaccaggatg aaactcatac accagatgag cggtttcttc
     8221 agaccgagtt tatcctgaac gatgccgtag aacatcataa atagaatgct ggtaaactgg
     8281 ttgaccgaat aaagtgtacc taattccgtc cctgtcaacc ctagatgtcc tttcagccaa
     8341 atagcgtata acgaccacca cagcgaccag gaaataaaaa agagaaatga gtaactggat
     8401 gcaaaacgat agtacgcatt tctgaatgga atattcagtg ccataattac ctacctgtcg
     8461 ttaaaaaatt cacgtcctat ttagagataa gagcgacttc gccgtttact tctcactatt
     8521 ccagttcttg tcgacatggc agcgctgtca ttgccccttt cgccgttact gcaagcgctc
     8581 cgcaacgttg agcgagatcg ataattcgtc gcatttctct ctcatctgta gataatcccg
     8641 tagaggacag acctgtgagt aacccggcaa cgaacgcatc tcccgccccc gtgctatcga
     8701 cacaattcac agacattcca gcaaaatggt gaacttgtcc tcgataacag accaccaccc
     8761 cttctgcacc tttagtcacc aacagcatgg cgatctcata ctcttttgcc agggcgcata
     8821 tatcctgatc gttctgtgtt tttccactga taagtcgcca ttcttcttcc gagagcttga
     8881 cgacatccgc cagttgtagc gcctgccgca aacacaagcg gagcaaatgc tcgtcttgcc
     8941 atagatcttc acgaatatta ggatcgaagc tgacaaaacc tccggcatgc cggatcgccg
     9001 tcatcgcagt aaatgcgctg gtacgcgaag gctcggcaga caacgcaatt gaacagagat
     9061 gtaaccattc gccatgtcgc cagcagggca agtctgtcgt ctctaaaaaa agatcggcac
     9121 tggggcggac cataaacgta aatgaacgtt ccccttgatc gttcagatcg acaagcaccg
     9181 tggatgtccg gtgccattca tcttgcttca gatacgtgat atcgactccc tcagttagca
     9241 gcgttctttg cattaacgcc ccaaaaggat catcaccgac ccgacctata aatccactta
     9301 ttccgcctaa tctggcgatt cccaccgcaa cgttagctgg cgcgccgcca ggacaaggca
     9361 gtaggcgccc gtctgattct ggcaagagat ctacgaccgc atcccctaaa acccatactt
     9421 tggctgacat ttttttccct taaattcatc tgagttacgc atagtgataa acctctttct
     9481 cgcaaaatcg tcatggattt actaaaacat gcatattcga tcacaaaacg tcatagttaa
     9541 cgttaacatt tgtgatattc atcgcattta tgaaagtaag ggactatatt tttataaaag
     9601 ttaacgttaa caattcacca aatttgctta accaggatga ttaaaatgac gcaatctcga
     9661 ttgcatgcgg cgcaaaacgc actagcaaaa cttcatgagc accggggtaa cactttctat
     9721 ccccattttc acctcgcgcc acctgccgga tggatgaacg atccaaacgg cctgatctgg
     9781 tttaacgatc gttatcacgc gttttatcaa catcacccga tgagcgaaca ctggggacca
     9841 atgcactggg gacatgccac cagcgacgat atgatccact ggcagcatga gcctattgcg
     9901 ctagcgccag gagacgataa tgacaaagac gggtgttttt caggtagtgc tgtcgatgac
     9961 aatggtgtcc tctcacttat ctacaccgga cacgtctggc tcgatggtgc aggtaatgac
    10021 gatgcaattc gcgaagtaca atgtctggct accagtcggg atggtattca tttcgagaaa
    10081 cagggtgtga tcctcactcc accagaagga atcatgcact tccgcgatcc taaagtgtgg
    10141 cgtgaagccg acacatggtg gatggtagtc ggggcgaaag atccaggcaa cacggggcag
    10201 atcctgcttt atcgcggcag ttcgttgcgt gaatggacct tcgatcgcgt actggcccac
    10261 gctgatgcgg gtgaaagcta tatgtgggaa tgtccggact ttttcagcct tggcgatcag
    10321 cattatctga tgttttcccc gcagggaatg aatgccgagg gatacagtta ccgaaatcgc
    10381 tttcaaagtg gcgtaatacc cggaatgtgg tcgccaggac gactttttgc acaatccggg
    10441 cattttactg aacttgataa cgggcatgac ttttatgcac cacaaagctt tttagcgaag
    10501 gatggtcggc gtattgttat cggctggatg gatatgtggg aatcgccaat gccctcaaaa
    10561 cgtgaaggat gggcaggctg catgacgctg gcgcgcgagc tatcagagag caatggcaaa
    10621 cttctacaac gcccggtaca cgaagctgag tcgttacgcc agcagcatca atctgtctct
    10681 ccccgcacaa tcagcaataa atatgttttg caggaaaacg cgcaagcagt tgagattcag
    10741 ttgcagtggg cgctgaagaa cagtgatgcc gaacattacg gattacagct cggcactgga
    10801 atgcggctgt atattgataa ccaatctgag cgacttgttt tgtggcggta ttacccacac
    10861 gagaatttag acggctaccg tagtattccc ctcccgcagc gtgacacgct cgccctaagg
    10921 atatttatcg atacatcatc cgtggaagta tttattaacg acggggaagc ggtgatgagt
    10981 agtcgaatct atccgcagcc agaagaacgg gaactgtcgc tttatgcctc ccacggagtg
    11041 gctgtgctgc aacatggagc actctggcta ctgggttaac ataatatcag gtggaacaac
    11101 ggatcaacag cgggcaaggg atccgcgtca ctcttccccc ttcacgacct tcaataatat
    11161 gcaatgcagc ttcccgcccg ataatgtcat gtggaagctg aattgtggtc agcggcggta
    11221 aaaacagatg cccgacgcca accagattat caaagcccat tacggcgaca tcctgcggga
    11281 ttcgtacccc cttcgccaga agaacctgat aagccacaaa ggctgcgcga tcgttaccac
    11341 atatcagaac atcaaaatct ggtttgcccg atttgaagtg gtcattgagt aaacttgcga
    11401 gatcggtgta gtgatcatca cctgttgcca tgtgaaattg tttcacctca gccagatctc
    11461 gtccagcatc acgccaggcc tgctcaaatc cctgccgacg ataccctgtt gccaacgcac
    11521 tttccggtag ccagaagcat aacggttgac gatagcccgc cgcgagcaaa tgctgtgttg
    11581 attcatattg tgcagtgtaa tcatcaggga tataactggg taacgctggg tcatccgcca
    11641 cacagttcgc caatacaata ttttcaccat acagagactc aggcagcgtg atatgtcgca
    11701 gccccattgt agtatagata atgccatccg gacggtgggc aagcagctga cgtgccgcgc
    11761 gggcagcgtc atcttcagaa aaaatattga ttaaaaaact attccagccg aactcgctgg
    11821 cggtttgctc aatggcaagc agaatatcaa cagagaaagg agtggtagcc gtgtcctgcg
    11881 ccagcacggc gagagtcgac ggcttacgtc cttgagcgcg catcttacgg gcggaaagat
    11941 caggaacata attcagggtc tggattgcct gcaatacgcg gtcacgcgtt gcaggacgca
    12001 cagattctgc attatgcatc acccgggaga ctgtcatcat cgacactccc gctaggcgtg
    12061 cgacatcctt taatgaagcc atacccaagc cgtttgccgt aaaacgggca ctgtagcaga
    12121 aacagacgtc actggcgaga tccaacgata ttctcaacgc cctatcaccc gacacggcaa
    12181 tacaataaaa aataacaata attgccggat aattgtactc aattccgcct ctgttctcgc
    12241 caacgagtaa ccatataggc aagtaagtaa aacatgccaa ctggcatcgc caacctggag
    12301 aagaaaatat aaatatggat cattgtgctg ccgctgtatc ccgacatcag cccggaaatt
    12361 attgcgattg ctatcggttc aggtgcaatt ggctgcacga tcgttacgga ctcgcttttc
    12421 tggctagtga agcaatattg cggcgctacg ctcaatgaaa catttaaata ctatacgaca
    12481 gcgacattta tcgcttcagt cgtcgctctg gcgggcacat tcctgctgtc atttatcatc
    12541 taagcgcaaa gagacgtact atggaaaacg ctaaaatgaa ctcgctcatc gcccagtatc
    12601 cgttggtaaa ggatctggtt gctcttaaag aaaccacctg gtttaatcct ggcacgacct
    12661 cattggctga aggtttacct tatgttggcc tgaccgaaca ggatgttcag gacgcccatg
    12721 cgcgcttatc ccgttttgca ccctatctgg caaaagcatt tcctgaaact gctgccactg
    12781 gggggattat tgaatcagaa ctggttgcca ttccagctat gcaaaaacgg ctggaaaaag
    12841 aatatcagca accgatcagc gggcaactgt tactgaaaaa agatagccat ttgcccattt
    12901 ccggctccat aaaagcacgc ggcgggattt atgaagtcct ggcacacgca gaaaaactgg
    12961 ctctggaagc ggggttgctg acgcttgatg atgactacag caaactgctt tctccggagt
    13021 ttaaacagtt ctttagccaa tacagcattg ctgtgggctc aaccggaaat ctggggttat
    13081 caatcggcat tatgagcgcc cgcattggct ttaagatgac agttcatatg tctgctgatg
    13141 cccgggcatg gaaaaaagcg aaactgcgca gccatggcgt tacggtcgtg gaatatgagc
    13201 aagattatgg tgttgccgtc gaggaaggac gtaaagcagc gcagtctgac ccgaactgtt
    13261 tctttattga tgacgaaaat tcccgcacgt tgttccttgg gtattccgtc gctggccagc
    13321 gtcttaaagc gcaatttgcc cagcaaggtc gtatcgtcga tgctgataac cctctgtttg
    13381 tctatctgcc gtgtggtgtt ggcggtggtc ctggtggcgt cgcattcgga cttaaactgg
    13441 cgtttggcga tcatgttcac tgcttttttg ccgaaccaac gcactcccct tgtatgttgt
    13501 taggcgtcca tacaggatta cacgatcaga tttctgttca ggatattggt atcgacaacc
    13561 ttaccgcagc ggatggcctt gcagttggtc gcgcatcagg ctttgtcggg cgggcaatgg
    13621 agcgtctgct ggatggcttc tataccctta gcgatcaaac catgtatgac atgcttggct
    13681 ggctggcgca ggaagaaggt attcgtcttg aaccttcagc actggcgggt atggccggac
    13741 ctcagcgcgt gtgtgcatca gtaagttacc aacagatgca cggtttcagc gcagaacaat
    13801 tgcgtaatgc cactcatctg gtgtgggcga cgggaggtgg aatggtgccg gaagaagaga
    13861 tgaatcaata tctggcaaaa ggccgttaat aacgtttcaa cgcagcatcg caatcctttc
    13921 cctgggtgag cgatgctgcc gatggcgcag acttaagatc cccgctctta cccgctataa
    13981 ccccctcctt ttatcatcac ccaacgcctt tcgctgtaaa cggcggtttc gcaaaccaaa
    14041 ccaaaaccgt aagtaagata aaagcaatag ccgccatacg gaaaatctca tttgcagaaa
    14101 tagaaagtga ctgctgggtt atttcattat taatttcatt aagaactcct gaaagcgaac
    14161 catagtattt atccataatt tgtgacgatg aattaaacac ggggttaaat tgatcgatgg
    14221 ttgctgtcaa ctgactatgg tgtaacgatt cgcgtcgtcc ccacagcgtc attgtcaacg
    14281 acgtaccaac tgatcctgac aaggtacgaa aaaaattact catactcgag gcattggcaa
    14341 atttattatc tggcaagcct gaaaacgaaa tcgttgttaa gggtaaaaag aaacaggcaa
    14401 cggcgaatcc ctggaaaaac tgtggcaaaa tgatgcctgt aaaatcaatc gttggcataa
    14461 atgtcacaga acgccagtaa tagcaaaccg catacatcaa aaaactaaat gtcactaaca
    14521 accgcatgtc tattttgttg ccataacgtc ctatcaaagg tgaaattaat agtggcatga
    14581 tgccgatggg cgcataagca agtccggccc atatcgcatt ataccccatc gtttcctgga
    14641 gtaactgcgg cataaggacg atcgctccag agtaaaataa atacgcgcat gtgatactca
    14701 caataccaat ggtgaagtta cgggacttaa acaaactgag atcaagaatc gggttctctg
    14761 aggtcgactc ccaaatgact aaagagatca gagaaataac tgatactact gttaatatta
    14821 ttattgtact cgagttgaac caatccagat cgcgcccttt atcaagcata atttgcaagc
    14881 caccaacacc gagcactaac agggtcagtc ctggtagatt cattttgacc ggtgaagttt
    14941 cagtttctct tcctttaagt aaggttaagc ataatgtcag gacgataatc cccataggga
    15001 cattgattaa aaatatccaa ccccagctaa agttatcgca aatataaccg cccaatatcg
    15061 gcccacatat cggagcgata atcacggtca ttgaccataa tgccagagca aatgttcttt
    15121 tttctggtgg ataattcctt aataataaac tctgtgacag tggaattaac ggccccgcca
    15181 ttaatccctg aacgactcta aaaaatatca gcacatcaag attggtcgat aaactacaca
    15241 ttaaggaaga cagcgaaaaa aaagtgactg aaagtaaaaa taaccttaat tcgcctattc
    15301 tttgtgccag cctgccggta acagggatcg caatggcatt tgctacgcca aacgaggtga
    15361 taacccaggt gccttcgtct gttgatgctc ccagaaagcc agatattgtc ggtattgcga
    15421 cgttagaaat agtggaatcc aacatttgca taaacgtcgc taatgacaat gcaatagtaa
    15481 cgcaccataa cgtcccaccg gttaatggtg ccggagtcga tttagtgatt gccattaaag
    15541 ttgtccatta tgcgaaataa tgttgctaat ttctttttct atcggactgg tatcgataac
    15601 taaagcctta ctggtataag ccggcatgga cgtcacggtt gaagccagat caggcatctc
    15661 agcaatgtct tcgttcttcg tatcaatagt tgcagtcatc gataaaccaa tgcgtaaggg
    15721 gtgttccatg agttcttttg gatcaagaga aacttcaacc ggtacacgct gaacgatttt
    15781 gatccagttc cctgtcgcat tttgtgcagg taataaggag aacgcattgc cggttcccat
    15841 attgatccct gtcacccgac catgaaacac aacattttca ccataaagat cgctgataat
    15901 attgactgat tgaccaatcc gtacatccgt gagttgtgtt tctttaaagt tggcattaac
    15961 ccacatttga cgtgccggta cgacagccat taacgattgt ccgggactca ctgtttcgcc
    16021 gacctgaaca ctccgctggg caatatagcc agtaaccgga ctccttatat ccgtacgttt
    16081 aagcgccaac caggcttctt tagttgcatc cgccgcttcc acgacttgtg gctgacggtt
    16141 taatggtgtg ttcattacta aagctttatt cgctttataa gcctggatag cggcattcag
    16201 tgccgctttg ctacttatta acgtatcttt ggtatgctcc agcgtttctt ttgaaataac
    16261 cccctgcttc gctaacggta ctcgacggtt ataatcttct aaagattgtt gatactgaat
    16321 acgtgctgaa gcgacttcgg cactgtattg tttatcctgt aagtacagtt tattcgtttg
    16381 ccgaacaata tttgccagat tatttttagc tttattgagt gcgatagtgg catcagtttt
    16441 atccagtgaa actaaaatgt caccttgtcg aacgtagttc gtatctttat gattaacgac
    16501 agtgacacta cctgagactt gtgcagaaat tggatctgca ttccccgtga cataggcgtc
    16561 atctgtacta atcatgtctt ctaattccat tgaccaatag gcataggcac ctgaaaacgc
    16621 aataaataaa actactgcca ataaagaaaa gtattttctt ctgttagaat gttttttatt
    16681 tgaattaatc tgttccacta ttatctctca tttctcatag atgaaattta tgaattgaat
    16741 gaaaagagca ttcattcttc atatcaaaca cagtgcatgt gttgcacatt atcccaggaa
    16801 cgttggaggc ctaatttaaa tgaggtgcaa aaaaaacaac agaggactaa accgtggctt
    16861 ttgcaataca attcttacgc ctgtaggatt agtgagaaga cttatagtg
//
LOCUS       DAZGJW010000099        16900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZGJW010000099 DAZGJW010000000
VERSION     DAZGJW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.099"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(82..366)
                     /gene="ppnP"
                     /locus_tag="ACHKIK_003319"
     CDS             complement(82..366)
                     /gene="ppnP"
                     /locus_tag="ACHKIK_003319"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO9084433.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(438..1115)
                     /gene="aroM"
                     /locus_tag="ACHKIK_003320"
     CDS             complement(438..1115)
                     /gene="aroM"
                     /locus_tag="ACHKIK_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO9084434.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(1373..1564)
                     /gene="yaiA"
                     /locus_tag="ACHKIK_003321"
     CDS             complement(1373..1564)
                     /gene="yaiA"
                     /locus_tag="ACHKIK_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO9084435.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(1614..2138)
                     /gene="aroL"
                     /locus_tag="ACHKIK_003322"
     CDS             complement(1614..2138)
                     /gene="aroL"
                     /locus_tag="ACHKIK_003322"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO9084436.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(2321..2779)
                     /gene="yaiI"
                     /locus_tag="ACHKIK_003323"
     CDS             complement(2321..2779)
                     /gene="yaiI"
                     /locus_tag="ACHKIK_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO9084437.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2899..3708
                     /gene="proC"
                     /locus_tag="ACHKIK_003324"
     CDS             2899..3708
                     /gene="proC"
                     /locus_tag="ACHKIK_003324"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO9084438.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(3725..4840)
                     /gene="adrA"
                     /locus_tag="ACHKIK_003325"
                     /gene_synonym="dgcC"
     CDS             complement(3725..4840)
                     /gene="adrA"
                     /locus_tag="ACHKIK_003325"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO9084439.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(4942..5262)
                     /gene="psiF"
                     /locus_tag="ACHKIK_003326"
     CDS             complement(4942..5262)
                     /gene="psiF"
                     /locus_tag="ACHKIK_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO9084440.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(5381..6796)
                     /gene="phoA"
                     /locus_tag="ACHKIK_003327"
     CDS             complement(5381..6796)
                     /gene="phoA"
                     /locus_tag="ACHKIK_003327"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO9084441.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITASGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(6897..7157)
                     /gene="iraP"
                     /locus_tag="ACHKIK_003328"
     CDS             complement(6897..7157)
                     /gene="iraP"
                     /locus_tag="ACHKIK_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO9084442.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(7339..7542)
                     /locus_tag="ACHKIK_003329"
     CDS             complement(7339..7542)
                     /locus_tag="ACHKIK_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084443.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            7620..8714
                     /gene="ddlA"
                     /locus_tag="ACHKIK_003330"
     CDS             7620..8714
                     /gene="ddlA"
                     /locus_tag="ACHKIK_003330"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO9084444.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(8738..8950)
                     /gene="yaiZ"
                     /locus_tag="ACHKIK_003331"
     CDS             complement(8738..8950)
                     /gene="yaiZ"
                     /locus_tag="ACHKIK_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO9084445.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            9210..9518
                     /gene="yaiY"
                     /locus_tag="ACHKIK_003332"
     CDS             9210..9518
                     /gene="yaiY"
                     /locus_tag="ACHKIK_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO9084446.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(9577..10671)
                     /gene="yaiW"
                     /locus_tag="ACHKIK_003333"
     CDS             complement(9577..10671)
                     /gene="yaiW"
                     /locus_tag="ACHKIK_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO9084447.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(10684..11904)
                     /gene="sbmA"
                     /locus_tag="ACHKIK_003334"
     CDS             complement(10684..11904)
                     /gene="sbmA"
                     /locus_tag="ACHKIK_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO9084448.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            12256..13413
                     /gene="ampH"
                     /locus_tag="ACHKIK_003335"
     CDS             12256..13413
                     /gene="ampH"
                     /locus_tag="ACHKIK_003335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO9084449.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMVPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(13414..14037)
                     /gene="iprA"
                     /locus_tag="ACHKIK_003336"
     CDS             complement(13414..14037)
                     /gene="iprA"
                     /locus_tag="ACHKIK_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO9084450.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(14125..16822)
                     /locus_tag="ACHKIK_003337"
                     /pseudo
     CDS             complement(14125..16822)
                     /locus_tag="ACHKIK_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556531.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         4293 a         4309 c         3981 g         4317 t
ORIGIN      
        1 cgcagcaaag gaaatattat tatgggttaa agagaggata tttcagaaaa atgccctccc
       61 gttcaagggg aaggcgagga attacagata gcggcacaga taagaggtgg gttcggcaac
      121 ttgcagatga aactcactgt gaccgggaac attaaacacc gaaccggctt catacacctg
      181 ccagtcggtc gcgtcaggca gtaacacatt cagcgcgcca ctgattaccg tcatctcttc
      241 cggctcagcg gtgctgaagg tgtattcgcc ttcaaccata acacccacgc tggcgcgacc
      301 agtgctgctg ctggaaaagc cgattgattt cactttgccg gaaaagtact cattactttg
      361 aagcataaac tggccctgtc tggtagttga acatctactt cactataggg gccagaggcg
      421 ctggctgtca cgcaaaatta caccagtaat tccgcagcca gccgtgcaat caatacgtta
      481 gacagcaaga caggaacatc gagctgtttt tgcagtaaat cgcgatgacg ttggtgaaat
      541 cctaaacaat ccagcatgat gacatcagcc ccttttgcca gtaattcttt cccggcatca
      601 atgatttttt gttctgaatc atgaatgggg ttgcccaatg aaaataccgg cgatttctgc
      661 aaaatttgcc atttttgcgc ctgaacggtc agcatctcct caaccggaac gataaccccc
      721 acctgatgat cttcaacaat agaggaaacc agtggaggca atattcgcga cggctcaaga
      781 aagatcgtat tacgcgcagt catactacta atgtttgctg tactcattaa taaaatgacg
      841 tcataaccct gattatcgag cacttcaacc acaccttgca ggtctcgctc cactttacga
      901 cgcgaaacat gggccagctg gttgtcattt aataatgtga gaatggtgtc ttcgcctgct
      961 tctggcgcgt actccgccat cacttcttca cgacttaact tccccagcaa gctatgatgg
     1021 gaaatattat cttcgtcaat gtattccgtc aggagcggca aaacctcctg cataggtaca
     1081 atgccgatgg tcaggatcgc caacgacgca ctcattgttc atccacctta cttttctttc
     1141 gtcgttaatt accgggcaag tgtgaagcac cactgctgac acttacttgt gctacaaatg
     1201 acaaaaagcg tagcagcaga cgtgcacggc ataatgtaaa gattccaaat gattccagta
     1261 atggattgtt attgtttaat attctaatta ttagaaaaac atgaattatg aaaaatgtga
     1321 cgcagatccc ttgtagtggc actcagaatc ccttccgagc agtctgatgc ggtcactctt
     1381 tgtcagggtc ctcatagcgt tttttcgcat ccatcgcttc ctgagccgtc ggatgttcac
     1441 tgatcaacga gtctggttta ggatgatcgg ctctgagttg gtaccaggtt accgtctgtc
     1501 ctggctttcc tttttcaatc gtcactatat atgcttcacg aggataaggc ggtttcgttg
     1561 gcatcgttct tccttatttc acgggatgaa cgttaagtat aggcgctcta aaatcaacaa
     1621 ttgatcgtct gtgccagggc gctacgaatt tcagaaatca cctggctggg ttcgtttgtt
     1681 gcgtcgatga taatatgcgc aacttcgcga tatagcgcat cgcgttcttc cagcacttcc
     1741 tgaacttctt cgctcagcgg ttttcccgtt aaggttggcc gtaaatcttc ttccggtgca
     1801 gcttgcagtc ggttaaccag gactgatact ggcgcacaca aataaaccac gatcccgtta
     1861 ttttgcatga agtgacgatt aaattccgtc agaataatgc cgccgcctgt agcgataacg
     1921 gtggatggcg cagttaccgc ttccagcgcc gccgtttctc tggcgcgaaa tcccgcccac
     1981 tcttcccttt cgacgatctc cgcgaccgtc atattgagct gtgattgcaa ccactgatcg
     2041 gtatcgacaa accgacggtt aagcgaatcg gcaagggcca ttccgaccgt tgttttacca
     2101 cagccccgag gcccgatcag aaaaagaggt tgtgtcatcg tgggttttcc ccaataggtc
     2161 gcaatgcggc gaaagccggt gtcatgagaa tagcgatcat gccatcaaac tagtacaatt
     2221 tcgattgtaa agaaaaaatt ccacttaaag tgaaaatctc aatacatccc ttactatacc
     2281 aataaatatt caagaatgaa gtgtaaataa taaattacat ttagccacga ctacgttgca
     2341 cttccagcca ccacttctcc agctccgcgg caaaggcctg gcggtcacgt tgtgaaaggc
     2401 tatctggtcc gccggtctgg atcccactgg cacgtaaggt atccataaaa tcgcgcatcg
     2461 tcaggcgctc acgaatggtc gctggcgtgt aacgttcgcc gcgcggatta agcgccgctg
     2521 cgcctttctc gatggcttca gcagccaaag gtatatctgc ggtgatcacc aaatcgcccg
     2581 cttcacactg ccggacaatt tcgttatcgg caacgtcgaa acctgccgcg acgcgcagcg
     2641 tacgaataaa tcgcgatggc ggcacgcgta aactctggtt tgctaccagt accagcggca
     2701 tctgcatacg ttccgccgcg cgatacaaaa tctctttaat tacattggga cacgcgtcgg
     2761 catccaccca aattgtcata aagtcatcct ttgttgggta atcctctatt gtgtcgcgct
     2821 tttgccttcc ggcatagttc tgtttatgct tctgccagcg attatcaaaa caatgaattt
     2881 cacggcagga gtgaggcaat ggaaaagaaa atcggtttta ttggctgcgg caatatggga
     2941 aaagccattc tcggcggtct gattgccagc ggtcaggtgc ttccagggca aatctgggta
     3001 tacaccccct ccccggataa agtcgccgcc ctgcatgacc agttcggcat caacgccgca
     3061 gaatcggcgc aagaagtggc gcaaatcgcc gacatcattt ttgctgccgt taaacctggc
     3121 atcatgatta aagtgcttag cgaaatcacc tccagcctga ataaagactc tctggtcgtt
     3181 tctattgctg caggtgtcac gctcgaccag cttgcccgcg cgctgggcca tgaccggaaa
     3241 attatccgcg ccatgccgaa cactcccgcg ctggttaatg ccgggatgac ctccgtaacg
     3301 ccaaacgcgc tggtaacccc agaagatacc gctgatgtgc tgaatatttt ccgctgcttt
     3361 ggcgaagcgg aagtaattgc tgagccgatg atccacccgg tggtcggtgt gagcggttct
     3421 tcgccagcct acgtatttat gtttatcgaa gcgatggccg acgccgccgt gctgggcggg
     3481 atgccacgcg cccaggcgta taaatttgcc gctcaggcgg taatgggttc cgcaaaaatg
     3541 gtgctggaaa cgggagaaca tccgggggca ctgaaagata tggtctgctc accgggaggc
     3601 accaccattg aagcggtacg cgtactggaa gagaaaggct tccgtgccgc agtgatcgaa
     3661 gcgatgacga agtgtatgga aaaatcagaa aaactcagca aatcctgatg actttcgccg
     3721 gacgtcaggc cgccacttcg gtgcggttac gtccggcttt ctttgctttg taaagcgcca
     3781 aatctgccga tttcaaccac tcacgatagt gactcatttg tgggttcagc ggcgcaaccc
     3841 ccacactaat ccgtaaagtt acctgtggcg tattcggcaa gcgtaacgtg tttagccctt
     3901 cacgcacccg taacatggcg gtaatggcgc tctcagctgg cgtaccggac atgattactg
     3961 caaactcatc gccgccaaac cgaccaatca catcgctacc gcgcagggta atttgtaact
     4021 gtcgggtaag cgccacaatc gcttcatcgc ccacatcatg accccaggta tcgttgatgc
     4081 tcttgaaatg gtcgatatcg ataatcagta acgttgcatc gcgattatgc cgccgacagt
     4141 tatcaaattc attgcgtaac atagtttccc aatgacgtcg gttatacacg cccgtcatgc
     4201 cgtcgcgggt actcatgacc tgcaacctgc gtttatgttc cgccagtttg gttgccgtct
     4261 ggtagctgac ccagccaaac agcagaggat aaacgacaat aatgggaagg gagagccacc
     4321 attccagcgg cgcactattg aacgacacgg taatgcccgt aagctcgagg gtgacaaggc
     4381 aggaaaccac catcaacacc agacccgcga caaacagacg ggggccgcct gcccccatca
     4441 aattcagaca cataatcatc aacatagcgg tggaaggcag cacgtttacg cccattacgc
     4501 ctacccacat tcccgctaat actgcatcgg tttttaagtt gtaaatttcc cggctaagcg
     4561 gatcgacggc cctgctcgct atctgccagg ctaaatgcgg ccagacgaac gcccagccga
     4621 ccaacaccag ccaccaccag cccggcggcg ggtgcgaaac cagcgttgaa gcaatcggta
     4681 agaacatgcc agccaggcca accgcacggg gtagtctgac gcgacgggcg aagcgcagcc
     4741 cggaccgctg atgttcgttt tgaggagtta aaggaggttc ctccccatgc gccgccgctt
     4801 ttttgaaaaa gttttcatca ttcattattt ttgggaacat tcagaaacaa ttttcccaaa
     4861 ttatagagac ggggctaaag gcagaagtat gaaatttcgg gtgagccact ttcgtagctc
     4921 acccgttagc actatcaggt atcaggcggc tttcttgagg caggcactca taaacttatt
     4981 acgatcatca cctttcagag attgttgcgt tgcttgatta ttacattcgc gcatcttttg
     5041 ctgctgtggc gtcaaacttt tttcaccagg cgcagacttg ctgttcttca ggcaatcact
     5101 catgtaggtc ttacgagcat cccctttcaa cgcctgcgcc gtcgcctgct gattacagga
     5161 tgtcatacgc tgttgttgtg gggttaaggt tctctcggca gcgccgacgg tggttaaaaa
     5221 aaccagacca aaaagcaagg taaccagtaa tgttattttc atagcaccat ccctcttcat
     5281 gttttaacca tgagcgtatg cgcccgtgat ctgccattaa gtctggttgc taacagcaaa
     5341 aaaaccaccc ggcagcgaaa attcactgcc gggcgcggtt ttatttcagc cccagggcgg
     5401 ctttcatggt gtagaagaga tcggtctggt cggtcagtcc aacaacattg gcggcatgcg
     5461 ggccatacgc cgcaatacgc aactgactgc cggtatgttc ttgtgaatcc tcttcggagt
     5521 tcccgtagct catcaccatc actgcgccat ctttggtatt tagcgcctgg gtgaggcccg
     5581 gagctttggt atccggcgcg acaatctggc tggcgtgggc gtgatcagcg gtgactatga
     5641 ccagcgtgtt gccatccttt ttagcgaatt ccagcgcccg ttgtacggct tcatcgagat
     5701 cgaccgtctc gccaatttgc ccacaaggat tcgcagcgtg atcctgttta tcgattgacg
     5761 caccttcaac ttgcaggaaa aatcctttct catttttact caacaattca atggctttgt
     5821 cggtcatctg cgccagggtt ggtacactgt cattacgttg cggattaggc gtacaggtga
     5881 ctgcgggctt atcgatattg ccgtggtacg ttgctttcgg tcctagccag cgcactggca
     5941 tattgccgtc agcaaacagt ccaagcagag gtttttgctg attcgcttcc gtcaccgaat
     6001 tcagtgaggc agcatcgctg accaactgat aaccacgcgc ctgtgcctgt tcacgcagcg
     6061 tttttccctg ccattcaccg gcggttgccg tttcagcaaa ggtttttgcg ccaccgccaa
     6121 gcgtaacgtc ggcacgagcg ttaagcagct gttcggtaat cgatcctttt ccgccttttt
     6181 ccagagcgtt acccggacat ttttcactgg tcgcgctcgg accgtagcat ttgcgcgagg
     6241 tcacatgcgc caccagcgca gcgggcgtgg catcctgcaa ctctgcggta gaaacgttac
     6301 cggtcgccag acctgcggct tttgccattt ccagaatcgt tgggtgatct ttttcgtgaa
     6361 tatcgacgcc cagcgcgccg ttataggttt tgacaccggt tgaccaggcg gttgctgatg
     6421 cagccgaatc ggtgacgtag tccggtttgc cggttttttt attcagcgca tagtgagtgt
     6481 attgcccggt aagcggtaag gcatcgatac ctttaaaaaa gccgcccgca ccttcggcat
     6541 aattacgtgc ggctgtaatt tccgaatccc ccatcccatc gccaatcagc aaaataatat
     6601 tttttgcagg tttatcgcta agagaatcac gcagagcggc agtctgatca cccgttaaac
     6661 ggcgagcacc gccggatgca gtaatatcgc cctgagcagc ccggttttcc agaacaggca
     6721 tttctggtgt ccgggctttt gtcacagggg taaacagtaa cgataagagt gccagtgcaa
     6781 tagtgctttg tttcacttta ttttctccat gtacaaatac attaaaaaat aaaaacaaag
     6841 cgactataag tctcggccgt gacaacttta tgacagctgt tgaaaagatt aactttttac
     6901 tgacgaggat gcttcaataa cttctttacg taatcgcgca gcagctccgt atcgtcgtca
     6961 ggaatgctgg catcgggctt tacctcgtac agcgccccct ctacctgatc aatcaagcgc
     7021 tgttggtcat tttgcgccat attgcgaagc attgcagtga cgataatctc caaagcttct
     7081 acctgcgcac acagttcttt cgactcttct tctttttggg caagcttaaa taacaactca
     7141 gcaatgagat ttttcatgtc tgtatttcct tatccaaagt atggagaagt taacattatg
     7201 tttcacaact tcatagtgtc tggtagtatt atttttatga caaaggaaat actttgcgca
     7261 aatatttttt gtttgattac cagcgaaacg tttctcttat ttaaataatt acccacacaa
     7321 aatataggct tttaattatc aacctttatt ataaataaaa gttatgaaat atattctctt
     7381 cgttaactta taatttagag aaaaaaactc accgcatggc catttaagcc aggaataaat
     7441 tagcttcccg ttcatcattt ttgtgatctt gcgcacatct ttgagataac gcactttctt
     7501 caccagtacg ttgcacgctt tgtcagcaga agattgcaac aatctttcag aaggccgcga
     7561 atcccccgtt ttgtcgtggt aatgtatgca ctttgtgtta gacgggatag gtttttaaga
     7621 tggaaaaact gcgggtagga atcgtttttg gtggtaaatc agcggaacat gaagtgtctc
     7681 tgcaatcggc aaaaaacatt gtcgatgcca ttgataaaag tcgcttcgac gttgtgctgc
     7741 tgggcattga taaacaaggg caatggcacg tcagcgatgc cagcaattat ctgctaaatg
     7801 cagacgatcc tgcccatatt gcgttacgcc cttcggcgac cagccttgcg caggtgccag
     7861 gtaaacatga gcatcagctt atcgacgcgc aaaacggtca gccgttgccg acggtggatg
     7921 tcattttccc gattgtccac ggtacgctgg gcgaagatgg ttccttgcag ggaatgctgc
     7981 gggtcgccaa tttaccgttc gtaggttctg atgttctggc ttcagcagcc tgtatggata
     8041 aagatgttac caaacgtctg ctgcgcgatg ccgggctgaa cattgcgcca tttattaccc
     8101 tgacgcgcgc caatcgtcac aacattagtt ttgccgaagt ggagtctaaa ctggggttac
     8161 cactgtttgt aaaaccggct aatcagggct cttctgttgg tgtcagcaaa gtaaccagtg
     8221 aagaacagta cgcaattgcc gtcgatctgg cgttcgaatt cgatcataaa gtgatcgttg
     8281 agcaagggat caaaggtcgt gagatcgaat gcgcagttct gggcaacgac aacccgcaag
     8341 ccagcacctg tggcgagatc gtactcacca gcgacttcta tgcctacgac accaagtaca
     8401 ttgacgaaga tggcgcgaaa gtggtagttc cggcagccat tgcgccagaa atcaacgata
     8461 agatccgggc gattgccgtt caggcttatc aaacgttggg atgcgcaggc atggcgcgtg
     8521 tagacgtgtt tttaacccca gaaaacgaag tggtgatcaa cgagatcaac acactgcctg
     8581 gcttcaccaa catcagtatg tatccgaagc tgtggcaagc cagcggtctg ggttacaccg
     8641 atctgatcac acgtttgatt gagctggcgc tggagcgtca cgctgcggat aacgcattga
     8701 aaaccacaat gtaatatttt aacgcccgat aatattctta ttgggcgttt tcagtttctt
     8761 catcgcgacg acgaataatc agccccgcca gccagaaact aatcacccac gtcaccagac
     8821 cgacggcata ggtctgccaa ccttttgctt caaatcccag taaccccacc acgccattaa
     8881 gaatgaatat aaggccaatg gcgaacgcat agtagtgcca gtcacggcgg atttttacag
     8941 gcagattcat ggcagctccg ttaacgaaaa acaacatcct tgcatattct gtaacaaccg
     9001 tctgtggtgt agacaacatt ttcgtaattg ttaataaatt taacttaagg ttgccatttt
     9061 gtgactcaca acgaagattc acgcttcggg aatattccca acatcaattt accatgattc
     9121 tgatattgac aatctaaatg acctgtcaga cttgccataa gccgctggta tcacaccaga
     9181 agagaagcag atgacttccg gaggttttta tggctgattt caccctgtca aaatcgctgt
     9241 ttagcggaaa atatcgcaat gcctcttcaa cgcctggcaa cattgcctat gcgttgtttg
     9301 tgctgttttg cttttgggct ggggcgcaat tgctgaacct gttagtgcat gcgcccggcg
     9361 tctatgagcg tttaatgcag gtccaggaaa caggtcgccc acgggtggaa attggtttag
     9421 gtgtcggcac cattttcggg ctgatcccgt ttttagtagg ctgcctcatt tttgcagtgg
     9481 tggcgctatg gctgcactgg cgacatcgcc gccagtaaga tttacgttgc cagatacggc
     9541 ctaaaacgcc ttatctggca tgacattcct gtttgatcac tgtttcatac aacgcgcccg
     9601 ccgttcgtct acgcgcttcg caaaccaggc cgtagtcagg ttgcgggtga ttttcgggct
     9661 ttccagttga atgccaggca acatttcgcg gggtagtgat ttacccgttt tcgcttcggc
     9721 aagttgataa actttcttgt acagcgccgt ctcctcaaaa gagaagctgt cacctttctc
     9781 taactgacgg cgaatttcgc tgtcgctcat ccccagtttt cccgccagtt tgcgcgttgc
     9841 cagttcggtt ttcccgggtt ctttactgtc gtaacgaatt aaatcaccgt ccagtgccag
     9901 cttcacgccg ctggccttac tgactgcgtt ctgaaacgcg gcattacggc tggcgtacca
     9961 gccagcgtta aaatcagcaa agcggtatat cggtgcgcta tagctggcgg gataattcag
    10021 taaatggtaa gtaccaaacc acaacccgcc gcggcgactg aagacttcct gacgaactgt
    10081 accgtccatt ttccacggat accctttggt atgctgttcg gcaaaagcaa tgctgacctg
    10141 catcggccca ccggtgcgca caggattaag cgaaccaaac agcgtctgcc ccattggtac
    10201 catgctgatc agatcgtcaa aaatcgcgct caattgcttt tctgtacgca ccgaatctaa
    10261 ccgttcgcta taacttttgc cgtttggcga tttgattttt agcgcggtat gaaccagaaa
    10321 agcaggaatg tgcatccgtt cagcacgacg gtcaatctct tgccaggcga tcttgcttaa
    10381 gcccggaacg gccggatctg cctgataatt tgactcctgc tgcgccaccg ccagcaccga
    10441 gcagacgttt tccagcgtcg gtgccagccc ctgactttca aaggtggtgg cgagatcttt
    10501 cgcccaggca tcgcggtctt tcacgctcgc aggcatcttc tgccgcacga cgctcgctac
    10561 atctatcgct ttttcgccct ttttcaacgg ttgcggagcc tgactactac accccgctaa
    10621 aaccagaacc gccaacaatg acagcgaaga tagatgattg acgcgcgaca ttacttctcc
    10681 tttttagctc aaggtatggg ttacttcctg aattttgtca ccatccagct catgttcaaa
    10741 gctgcgcaga cgtttgtaga tagacatcaa ctcaaccagt gtggtccatg agttaatcag
    10801 gtactggaaa gcaccgcgaa cctgaccaaa aacgttggta atctgcgtca tcaggccgag
    10861 cgtaatcgta ccggcaacaa ttgacggaaa cagcaagaac aaaccgaaaa cgttatcgac
    10921 ctgcaaatag agaatgcggg cgatgttgaa atacatatag tgaaaataga ggcgaaaata
    10981 gtttttccgt acggcgctaa acagctcgcg taccgtaggc ggcgtcgcgc gcgtggcatc
    11041 gtcttcacca taaaccagct ctttacggta ggcggcctct actcgctggt ttttaaactc
    11101 cagccccggc agtttgatcc ctaccactgc cagcaatccg gtccccatca gcgaccagac
    11161 gatagcggca atcaccagac catacggaat gtgcccgata atcggcagct ccggcacatg
    11221 cgcggagagc gttaccagca ccggcagaaa ggcgatcaac gtcatgatgg cgttgataaa
    11281 actgacgccc atattctcca gcgttgaagc aaaacgcatg gtgtcttcct gcacacgctg
    11341 tgcggcacct tcgatatgac gcagttgttg ccagttcgcc atgtaatatt cgttcatcgc
    11401 cgtacgccag cggaacacat agtgactgac aaagaagttg ttcagtacac tgatcaccac
    11461 cgcgatcagc gcaatcccca gaaagacgcc cacttcgcgg taaaattgtt cgatagtgac
    11521 tttatgcggc gaacttagcg cggtttgaat cagatcatag aacggtgcat accaggcgtt
    11581 gacggcgacc ccgacttcca ccaaaaacca ggtgacgaag atgatcagtg cagtaccgag
    11641 tattgaccag tattgccaac ggtgcgggct gtagataaac cagaaaaatg caaaaagtcc
    11701 tacgcaaaca atgtagtaag cgtaaaaaat caggaaatcc aacgaccaga aacgcgcggc
    11761 gctaatcggg atctgcccgg aagcgccggt gatacgcgcc acccagtcac ccccaccggc
    11821 ttgccagaag ataacggcaa tcaatgccca aacaaaggcc gagagaaaaa acgttcccgg
    11881 ctttgggaaa aaagacttaa acatatgcac tcctgctaac ttcttatcgt tttaacaact
    11941 gctgtgtatt aaccgcatga ccgctacaaa gcgtcagcca aatgaaaccg cccattgtga
    12001 cccatagcaa ggcgcttagt tcgccagcct gaccataaga attgtttcga cggatttcac
    12061 aaaccaaaga ttgtttacat aagcggatta acacgcgcag gatcacaaat gcaggcattg
    12121 gtgagcagga aaaagatcgt gtttacgcgc cgttacgttt ttaacactat tattgcacta
    12181 attcgccctt tgcaatccat caatgagtag tataaatacg ctcagttacc ttcattcaat
    12241 ctatggacac caccgttgaa acgtagtctg cttttttctg ccgtgctgtg tgcggcgtca
    12301 ttgacctccg tccatgcggc gcagccgatc actgaaccgg agtttgcctc tgatattgtc
    12361 gatcgttatg ccgatcatat tttttacggc agcggtgcca cggggatggc gctggttgtt
    12421 atcgacggta accagcgcgt ctttcgcagt tatggcgaaa cgcgacctgg taataacgtt
    12481 cgcccgcagc tggattcggt cgtgcgtatt gcttccctca ccaagctaat gaccagtgaa
    12541 atgctggtga aattgctcga ccaggggacc gtgaagttaa acgatccgct aagtaaatat
    12601 gctccgccag gcgcacgcgt gccaacctac aatgggacgc ccattacgct ggtcaatctg
    12661 gcaacccata ccagcgcgct gccccgtgaa cagcccggtg gcgcggcaca tcgtccggtg
    12721 tttgtctggc caacgcgcga gcaacgctgg aaataccttt ctacggcgaa gctgaaagcc
    12781 gcgccaggca gtcaggcggc gtactctaac cttgcgttcg acctgctggc tgatgcactg
    12841 gcgaatgcgt ccggcaagcc ttatacccag ttgtttgaag agcagattac ccgtccattg
    12901 gggatgaaag acaccaccta caccccctca ccggatcagt gccgccgttt aatggttgca
    12961 gagcgcggtg ccagtccatg caataacacg ctggcggcaa ttggcagcgg cggcgtctat
    13021 tccacgcctg gcgatatgat gcgctggatg cagcagtatt tgtcatctga tttttatcag
    13081 cgcagtaacc aggcagatcg catgcagacg ttgatttatc agcgcgcgca gtttacgaaa
    13141 gtgattggca tggatgtccc cggcaaagcg gatgcgcttg gtttaggctg ggtatatatg
    13201 gtcccgaaag agggtcgtcc ggggattatt cagaagacag gaggtggtgg cggtttcatt
    13261 acctatatgg cgatgatccc gcagaagaat attggcgcgt ttgtggttgt cactcgttcg
    13321 ccgttgacgc gctttaaaaa tatgagcgat ggcatcaatg acctggtgac cgagctaagc
    13381 gggaataaac cgctggttat ccccgcgtcc tgattaatac tctgaaggca aacggttgat
    13441 agcgaccagt ttgcctttat tcatttcgat atagccgcct ttacgcaaag cagcgagaac
    13501 ttctgcgacg acagaacgcg atatgcgtgt acgttgatgg atataattca tcacaccaat
    13561 acgtgatcgc aattcttcat tccagtcaat cattgataat aatgttgcgc ggatttgttc
    13621 gtaggaatta tgcccaatga gctgcacgtc gcgtaattcc agaattctat tttgccaggc
    13681 taaccagtaa aaagcgtctc gccagagttg attttgttca ataagcgtaa tggtttgttt
    13741 ggctggtaga tgatatcccg tacaatttcc ttctgatatt aatttgtatg gtatatcgtt
    13801 tttcattaaa ccatcagcca gccccataat ataaggagcc tgggtaatac cgataagtac
    13861 gttttcttct ctacgcagag agataactcc ctctaaaata acgaaagtat cttcgttatt
    13921 gacatcactg gaaaatataa cttgcttttc attattaaac tcgaagcgcg taccgtatct
    13981 ggacaaacat ttatcgagct taccaaattc ctgaagaggt ttaactacag ataacatttg
    14041 cgcgtccttt gcagtaatgc ccgtcaaatc cttgacgggc attatttaga ttaaattacc
    14101 agaatttctt cggagtgaag aatattacca ggtatattta acacccacgt tcgcggacca
    14161 gtcttgatct acgtcaccac caccgaggta gttagcatcg gtataggcgc tgaagttctt
    14221 ggtgaagctg aactgagtac ccagaccaac acgtaccgca gacccttcag taccgttatc
    14281 gatggaatca ccgttcacat cgttatcgtt gttagagtcg tcgtagacgt aagccagttt
    14341 gaagtacgga gtcagagcct ggtcttcgct gtaggtgaag gtataacctg catctacacc
    14401 cagttcataa cgcatgctgt cgtaagactg accgtcaact ttcatgtcgt tgctcagctg
    14461 gtagtcatca ccagactgga acagaccaga aacgctgccg taaggagtca cgtaaccagc
    14521 atcacccagt ttgaagtcgt aaccggcttt caaaccgaag ccccaagcgt cggagttggt
    14581 gctaccgtca acgtaagtac cgttgctcat ggttgcagac aggtcgttgt tgaagtgaga
    14641 gtagctcaag ctaccatcaa caaagacgtt gttcgcgaag tgagcagaag agtagatgta
    14701 ggcagtctgg ctgtcttgat ccacctgacc agaacggtca ttcatgtcac ctttagcgaa
    14761 gcctgcagcc gcaccgacga tccacttagc gttgttaccg tcaattttgg tatcaacacc
    14821 gaccatgatg ccgttaacat cctgatcata gttgatggtg ccgttgtcgc cgttgaagtt
    14881 accaccgaag tagcttaccc atgcgccgcc gttatcagcc aggccatgac gagagttggt
    14941 cagacgagta ccaacggtgt cttgttccag gttccagata ttggtgttcg cagacgggat
    15001 gctcagcgcc atgttagcgt agtcggtcag ctccatctgt tgcagaacaa cggtgttacc
    15061 gcgctgttca gcctgatagg tgtatgcacc caggtcagct ttgttagcag cagagaaggt
    15121 cgcgttggtg ttgacgtcgt ttacgtagat aatttcttta tctttgtaat cagcgatagc
    15181 gccagcgcct gttgcgttgt cgatacgaac tttgtagtta ccggttgcag caacaacgtt
    15241 gtcaacagag ctgttatcca gttcgtcgcc gttgttcatg tcaccgttac cgttgatagt
    15301 cagatgacca tcagagttca tagcaacaat accgtagccg tagttagact tggaagtatc
    15361 ccaggtacgg tcgttcacca aatcagcgtt cagaacataa tcgctgctgt ggatatcgaa
    15421 tacaccagac actacgttgc cgttatggtc agcgatgttg ccgctggtca gttccaaagt
    15481 atcagtctgg aacggattat catcgaactg gtctacatta atatccagta aaccaccgtt
    15541 agtcacagtt attgtatttg ctaacagcga cggagtgtaa taaccattat agatacttga
    15601 attgtcatca ctagacaacg ctacagtact gtagctatca actgtcagat gatcagttcc
    15661 aacataacct tcatctccaa tgttcaaaga agaaccattg gtcagagcga tgctatcaga
    15721 aatcaagcta gattcagaca cattaacctg agagccatta ttcacagcta aagtatcaat
    15781 gtttgaagcg ttaacggtat tccattcaga accattattc agagtgacgt taaacacacc
    15841 gctctggaat acttgatcat caatcgtacg accccaagca tttgcttctg ttacaacgcc
    15901 cggatacagg ctattacctg taacatcata ccagtcagtc gaagcagtag attcatcatc
    15961 aacatttgct gatgtagtag ctgagcctac ccacttagaa ccattatcca gagtgatatt
    16021 cagttcatca acattaagtg agtcatcagc ccagccacca ttggtgtcca agtacgccat
    16081 cggcattact gtcatgacct tgaggataga agtttgcgtt gaaagtactt gcgaaaacaa
    16141 catcacccag cagagtagaa ttgctgaaat aaacattgtt tttcatggag taatttgaag
    16201 ccgcactatc actgaaatac agagctacat cattagcttc gccgttacca gagtaatcac
    16261 taggttccgc agagttacca tagaaaccac tgtcttccag agtagttaca gaacctgaag
    16321 tcactgtgga atcggtaact gagatattgt tatcccaagt atgagattca gtagtgtaag
    16381 tattgtttac ggtattaccc tgagtcaggc tgataccagc aacgcgggag ttgttagaaa
    16441 tattgatgtt actttcgaca tcaagattca ctgccagccc aaggccaaca ccaccgtttg
    16501 caaacaaacc ggcgtattct tcaccttcac cagctggggt atggaaggtt tcgttagtat
    16561 atttggtaac gtcaccgtcc agatatgaat cagtgaagta taaaccttca taatcgtcat
    16621 caattgtaga attagcaata tttaaagtga atacatcacc atcatgccaa ttatcatcaa
    16681 ttacacgatc agtaccgtat tcagagaact gatagacata atgaccatca ctgctttcac
    16741 ccataggcag cattgaagtg attgaaccat gaatcaggga gttgctgatg ttcaactggt
    16801 ttgttgttcc atcagtaaag ttgatcctac ccacgtaata tggacacagg cctaagcgag
    16861 gttcttgttt tcaaattgtt ccggactgag gccgccacac
//
LOCUS       DAZGJW010000100        16756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZGJW010000100 DAZGJW010000000
VERSION     DAZGJW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.100"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(354..695)
                     /locus_tag="ACHKIK_003338"
     CDS             complement(354..695)
                     /locus_tag="ACHKIK_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO9084451.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(708..1580)
                     /gene="copD"
                     /locus_tag="ACHKIK_003339"
     CDS             complement(708..1580)
                     /gene="copD"
                     /locus_tag="ACHKIK_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO9084452.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(1584..1958)
                     /gene="yobA"
                     /locus_tag="ACHKIK_003340"
     CDS             complement(1584..1958)
                     /gene="yobA"
                     /locus_tag="ACHKIK_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO9084453.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            2097..2327
                     /gene="holE"
                     /locus_tag="ACHKIK_003341"
     CDS             2097..2327
                     /gene="holE"
                     /locus_tag="ACHKIK_003341"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO9084454.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            2429..3085
                     /gene="yobB"
                     /locus_tag="ACHKIK_003342"
     CDS             2429..3085
                     /gene="yobB"
                     /locus_tag="ACHKIK_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO9084455.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            3109..3771
                     /gene="exoX"
                     /locus_tag="ACHKIK_003343"
     CDS             3109..3771
                     /gene="exoX"
                     /locus_tag="ACHKIK_003343"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO9084456.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(3768..5828)
                     /gene="ptrB"
                     /locus_tag="ACHKIK_003344"
     CDS             complement(3768..5828)
                     /gene="ptrB"
                     /locus_tag="ACHKIK_003344"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO9084457.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCIDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(6037..6696)
                     /locus_tag="ACHKIK_003345"
     CDS             complement(6037..6696)
                     /locus_tag="ACHKIK_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO9084458.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(7023..7379)
                     /gene="yebF"
                     /locus_tag="ACHKIK_003346"
     CDS             complement(7023..7379)
                     /gene="yebF"
                     /locus_tag="ACHKIK_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO9084459.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(7446..7736)
                     /gene="yebG"
                     /locus_tag="ACHKIK_003347"
     CDS             complement(7446..7736)
                     /gene="yebG"
                     /locus_tag="ACHKIK_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO9084460.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDSADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            7870..9048
                     /gene="purT"
                     /locus_tag="ACHKIK_003348"
     CDS             7870..9048
                     /gene="purT"
                     /locus_tag="ACHKIK_003348"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO9084461.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEQLQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGLQIRLFGKPAINGSRRLGVALATAESVVDAIKRAKHAAGQVKVQG
                     "
     gene            complement(9104..9745)
                     /gene="kdgA"
                     /locus_tag="ACHKIK_003349"
                     /gene_synonym="eda"
     CDS             complement(9104..9745)
                     /gene="kdgA"
                     /locus_tag="ACHKIK_003349"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO9084462.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(9782..11593)
                     /gene="edd"
                     /locus_tag="ACHKIK_003350"
     CDS             complement(9782..11593)
                     /gene="edd"
                     /locus_tag="ACHKIK_003350"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO9084463.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(11828..13303)
                     /gene="zwf"
                     /locus_tag="ACHKIK_003351"
     CDS             complement(11828..13303)
                     /gene="zwf"
                     /locus_tag="ACHKIK_003351"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO9084464.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            13641..14510
                     /gene="hexR"
                     /locus_tag="ACHKIK_003352"
                     /gene_synonym="yebK"
     CDS             13641..14510
                     /gene="hexR"
                     /locus_tag="ACHKIK_003352"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO9084465.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14638..16080
                     /gene="pyk"
                     /locus_tag="ACHKIK_003353"
     CDS             14638..16080
                     /gene="pyk"
                     /locus_tag="ACHKIK_003353"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO9084466.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(16140..>16756)
                     /locus_tag="ACHKIK_003354"
     CDS             complement(16140..>16756)
                     /locus_tag="ACHKIK_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFO9084467.1"
                     /translation="GDVLEFSWARKIMDYTRGELSYLKIIEQKKEFLHKNNLVIIRCH
                     DVMDREPTFGMSKALAQQLELDVTNIVASDDMYHVYAIEPDSAINITKRFAKNLKIYN
                     LPGIQFYGDRARIVRTIGIGAGCFCDPIQYMEYQADYYITINDSIKTWVQTQYSKDSG
                     LPMAVIGYSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDYDWIE"
BASE COUNT         4168 a         4286 c         4086 g         4216 t
ORIGIN      
        1 atataaccaa aaaagaccgg aatacagaaa ttcgagtaaa tttcgagtaa tttcggggag
       61 acgtttgcaa ctaattgatt ttaaatacaa ttaaaaaaag accgaatacg attcctgtat
      121 tcggtccagg gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag
      181 gcttagttgc cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca
      241 aaatggtcaa taaaaagcgt ggtggtcatc agctgaaatg ttaaaaaccg cccgttctgg
      301 tgaaagaact gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag
      361 agtttttcag ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga
      421 tcatcaatct gaatcaccga aatgggttgg gctttagtct tcccactggc aacttccttt
      481 tgtgcgatat cgtttaaagg atactgcacg agggtacttg ggttaatgac atacaaagca
      541 ttacccggtc tgcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc
      601 acttcaaaac gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg
      661 agtaacagaa acgtcagaat actttttttc atcattttcg taatccatca aaaaggttcg
      721 agagtcgcaa gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta
      781 gtcatccact caaaccaggg tgccgcacga tcttcatctt gcctcatgcg cggtacaaga
      841 acataacgat tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat
      901 aagagttggc cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta
      961 agcacgccgc ttgccagtac gccgatcacc gcaaaatgcc cgcaccagga aaaacgcatc
     1021 agcgcctgaa tggcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc
     1081 ggcagtaatc ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga
     1141 tggattttcg ccgttacccc ttcattcagt gtcgcatgcc cgattcccgc cagcagaata
     1201 aattgcgcgg tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg
     1261 acgatcaacg ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt
     1321 tgcaaaacag cctgccaaat gtttggcgaa aatacatctg cccatcccgt tcccatcaac
     1381 ccgccctgaa cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt
     1441 tgtaaacgca gaaaacgctt cgccagcaag cgacgaatcg ttaagggggc cagccaggca
     1501 ccgtacattg cgaagccaaa gaccagcatc agcgaggtga aatggataaa tcgcagcgcg
     1561 atccaggtaa acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta
     1621 tgcccgtcca ccgaaacaac atgccagtca acgatgtagg ttccgggttt cagcgaatcg
     1681 gccagtggaa caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta
     1741 atattttcgt tttttggccc cgtgattttt gcaccactga atcctgtttc aacaccttcc
     1801 gagaagttta aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac
     1861 tgatgcgtta aatgcgcatg tgcccagacg gaaggtgtca ccaacgaagt ggtcaggatt
     1921 gccagcgcgt agcgaaggga gcgtgcagtt gaagtcatat tatctattcc tttttgtaat
     1981 aactttttta cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct
     2041 tgccatcctg gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc
     2101 tgaagaatct ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg
     2161 cggccggggt ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac
     2221 gtgaacagcc tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg
     2281 cttcggtcaa tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacagag
     2341 ttacactttc ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat
     2401 ttgcagtcat tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata
     2461 tgagcctcgt aaaacctcac tcaccgaaca ggtagctcat catctggagt ttgtgagggc
     2521 cgccgccagg caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga
     2581 ttattcgcga cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta
     2641 tgccgcaacg acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg
     2701 ctttattcgt ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca
     2761 gagtcatggt gcatgtctgg gtcgtcgttc cagaaccatc acggtggtcg atgaacaacc
     2821 gcaaggtatg gatatggacc cgacctgttc actgtttact acagggcaat gtctgggaga
     2881 acccgacctg ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc
     2941 cgtgctgatg gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat
     3001 cgttcgcgcc gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca
     3061 aggcgatatc attccattac gctaggcttt ttcggcctgg agcatgccat gttgcgcatt
     3121 atcgatacag aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc
     3181 attgacggaa aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt
     3241 ccacaagcga tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt
     3301 gaagatgtga tcccacacta ttacggtagt gaatggtatg tcgcgcataa cgccagcttt
     3361 gaccgccgcg tactgcctga gatgcccggt gagtggattt gcactatgaa actggcccgt
     3421 cgtttgtggc ctgggatcaa gtacagcaat atggcgttat ataaaacacg caagctcaat
     3481 gtacagacgc cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc
     3541 gcgttgctta tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc
     3601 accggacgtc cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt
     3661 tccgacgttg ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc
     3721 atgagcccgg agctgcgttt aacactgaaa cattatctgg aaaatactta gtccgcaggc
     3781 gtagcgggta atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct
     3841 tcgtacgatt taaagcgacc agatttaccg ccatggcctg agtccatgtc gatacagagc
     3901 aataaaagat ggtcatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc
     3961 caatattgca cctgagaatc gtgcaaaccg gtcgttacca gtaaatgcgg ataagcctgt
     4021 gcggtgacgt tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc
     4081 ggattacccc actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt
     4141 gttacaacat caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga
     4201 ttaattgcaa cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa
     4261 ggagagccat agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta
     4321 tttttcttct tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg
     4381 cgaacatgga caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg
     4441 aaatcggcat caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt
     4501 cctttgcgaa aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg
     4561 gcgactatcc acaggtgttc actgcggtaa ttcgccgcat caaaaccggg cacttctgtt
     4621 tgttttaata cacgacgctc accggtatcc atatccagtt caaacaaagt gtctggtgtg
     4681 gtcatggaag aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc
     4741 caggtcacat aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta
     4801 atttggcgca aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac
     4861 agcgtaaacc cttccagcat gatgttttcg cgtggcggaa ttaactcttc ccactgttgc
     4921 tcatcacgca tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgtaga
     4981 taaaaacgat gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa
     5041 acaaacggct cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg
     5101 ctggccaaat gaatgactac atagtgcttc gaggtcgttt tatgcaggct gacgtaatag
     5161 gtatcgtctt tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga
     5221 cgccagacct gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag
     5281 gagtcatttg cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta
     5341 ccagtttcca gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc
     5401 agcgccataa tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta
     5461 tgagctgcgc gcttattggc atcgagcaat gtttcccact catcccactc ttcactgaat
     5521 gccgattgac gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg
     5581 tagccatttt tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc
     5641 tttaagatgc gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt
     5701 tcttgttgca ggtagtccag gacttctggc tgagaacgcg tatcgtcccg cagccagtag
     5761 taattatcga tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt
     5821 ggtagcatgt tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg
     5881 ggatgcaagc gaaacagggg agtcattgct tagatgatga caggtaatgg cgcggatatc
     5941 gaatgttatg cagacagaga aaatcagcct gttcaaatgg ctgtgcgatt ctggatagcc
     6001 cgaaatagtc aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg
     6061 ctgttcgaga tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc
     6121 cagtgcattc aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag
     6181 gaaatagatt tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtggatc
     6241 cagcggttgt tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc
     6301 acgcaattgc tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc
     6361 ggcaaagacc agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc
     6421 gccaaactgc ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag
     6481 caccgtaccc gctaccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag
     6541 aagtttacgt gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg
     6601 cactaacagt ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc
     6661 aagcagggat tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt
     6721 agtgcctgta gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc
     6781 aaataagcgg ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg
     6841 tatatcaggt gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt
     6901 tgatgctgat tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt
     6961 tctggggcgg acaaggcact cgcgccgcat ccgccaacgg tcaatcgaat tatcagaagc
     7021 cattaacgcc gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga
     7081 atatcggcac ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga
     7141 atgtcctgca aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct
     7201 gcccaacgcg ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca
     7261 gcatccagac cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg
     7321 aaaactgatg cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg
     7381 tttttctcca tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag
     7441 ttgcttcagg ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg
     7501 gacggtaatt tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat
     7561 agcgaaaggg cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg
     7621 tcaagcagat ccgccgaatc aagcatttta tcataagcat cggcttcctt tttgctggta
     7681 aacgacattt tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc
     7741 agcctctcat aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca
     7801 tcagcaataa agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg
     7861 atattcgtta tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta
     7921 ttaggctccg gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag
     7981 gtgattgccg tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat
     8041 gtcattaata tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat
     8101 tatatcgtgc cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa
     8161 ggactgaatg ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc
     8221 cgtcgcctgg cggcagaaca gctgcagctg cccacttcca cttatcgttt tgccgatagc
     8281 gaaagccttt tccgcgaggc ggttgccgcc attggctatc cctgcattgt aaaaccggtg
     8341 atgagctctt ccggcaaggg gcagacgttt attcgctctg cagagcaact tgctcaggca
     8401 tgggaatacg ctcagcaagg cggtcgcgcc ggagcgggcc gcgtaattgt tgaaggcgtc
     8461 gttaagtttg acttcgaaat taccctgcta accgtcagcg cggtggatgg cgtccatttc
     8521 tgtgcaccag taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag
     8581 caaatgagcc cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca
     8641 ctgggcggtt atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc
     8701 agtgaggtct cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctc
     8761 tcagagtttg ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgccag
     8821 tatggtcctg cagcttctgc cgttatcctg ccacaactga ccagtcagaa tgtcacgttt
     8881 gataatgtgc agaatgccgt aggcgcaggt ttgcagattc gtttatttgg taagccggca
     8941 attaatggca gccgtcgtct gggggtggca ctggctactg cagagagtgt tgttgacgcc
     9001 attaaacgcg cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa
     9061 aacgctacaa aaatgcccga tccgcgatcg ggcattttga cttttacagc ttagcgcctt
     9121 ctacagcttc acgcgccagc ttagtaatac ggtcgtaatc gcccgcttcc agcgcatctg
     9181 ccggaaccag ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt
     9241 agttagccgg agaaatacca cctgtcgggc agaaacggac ctgggagaac ggacccgcga
     9301 tcgcctgcag ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca
     9361 aaccgtagtc catacccagc atcagttcgg aaacagtgct gatccccgga atcagaggaa
     9421 tagtcccttc ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact
     9481 gtgcacccgc ttcggtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca
     9541 caatcgcttc aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac
     9601 gcagagtcac ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg
     9661 cgtgttccag tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt
     9721 ctgcacttgt tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt
     9781 cttaaaaagt gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc
     9841 tgaataattc acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc
     9901 gagcagccag ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac
     9961 gaatgatgtc cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta
    10021 cgtggatagc tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg
    10081 caattttgaa acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg
    10141 gcattccgtt cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca
    10201 aacccgcttc aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt
    10261 caatcacctg gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac
    10321 cgcttaacac ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat
    10381 tgctgtcgag tgatttttcc gccccttccc gccagtccag ttcaccatta ttcagccatg
    10441 gttcaagggt ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca
    10501 ggcctgcttt gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt
    10561 taatatcggc cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt
    10621 cagagaagtc atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca
    10681 tggtgtggtt agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt
    10741 tctcatcgat catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa
    10801 cctgacgcgc agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag
    10861 aagagcctgg caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac
    10921 cgtagaaagt acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca
    10981 tgcggtccac tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca
    11041 aaccgcttgc catcggtcca gacggcacaa acaccgcagg caaatgacca aacgacaggg
    11101 ctgccatcgt cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa
    11161 acatgttatg ggacagcccg accgccgcag acatcgctat cacttcgcgg cttagcagcg
    11221 acaattccat tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg
    11281 caacctgacc aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt
    11341 cataaggctg gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac
    11401 gcaacatgct tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca
    11461 ggttaccgca tgccaactgc gaacgatgaa cggtcgaagt tttcgcttgt tctatccggg
    11521 cgagataagc agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcgtaaca
    11581 attgtggatt cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg
    11641 tttcattcag aggatttatg actgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt
    11701 ttcagtcagt gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa
    11761 aaattgttct acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc
    11821 gcagatatta ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg
    11881 caacgggtcc ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg
    11941 cctcagtaat ggagtctacc catttccagg cttcttccac ttcgtcgcga cgaacaaaca
    12001 gtgcctgaat accacgcatg gtttccagca gcaaacgttc ataggcatcc gccagatgcg
    12061 tctgattaaa ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt
    12121 ggtcaaggcc aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac
    12181 ggatagtcag tttattctgc ggcagatcct gccacgattc tttaaacaga ttcagttcag
    12241 gtgttttgaa atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca
    12301 ggtagaatgg cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gctacgaagg
    12361 tttctgtatt gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc
    12421 cctgggcgaa gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt
    12481 cgatgcggcg cagagacttc agtactttca ctttttcatc gcggatgctg tctgcgctca
    12541 ggtcagacgg cggagacatc gcaatcatgc agagaatttg cagcaggtgg ttttggatca
    12601 tatcgcgcat ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt
    12661 ctgccacggt aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg
    12721 agttagcaaa acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga
    12781 tacggtaaac ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg
    12841 tcgccagcga cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt
    12901 tagcctcgcc aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt
    12961 taatggtgat acgatttttt tgatccagca tcgcgccgag acggctgaat gcagcagtgt
    13021 cattgacatc gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat
    13081 caatggtttc tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt
    13141 tatcccagtc agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt
    13201 tttccagttg atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga
    13261 aaatgaccag gtcacaggcc tgggctgttt gcgttaccgc catgtcattc tccttaagtt
    13321 aactaacccg gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc
    13381 ttttacgagc ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg gtaaaagcgc
    13441 gcaaagtgcg gcaaaactga taaaaaaatc atcgttttcc attgggtaaa aatctgacac
    13501 tgatcatgtt atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacagcac
    13561 acaggcgtaa tatctgacaa aactacattt cgatttcttt cagtgcggaa atcgtcatta
    13621 cccgtgagtt tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt
    13681 tgagcaaatc agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc
    13741 attcgagtat tgctgctctg gcgctggaag ccaatgttag cgaaccgacg gtgaatcgtt
    13801 tctgtcgcag catggacacg cgcggttttc ctgattttaa acttcatctg gcacaaagtc
    13861 tggcgaatgg cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat
    13921 acacagggaa aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg
    13981 ataaatctgc catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa atcgcctttt
    14041 tcggattagg ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta
    14101 atgttccggt ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta
    14161 gcgacggaga cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc
    14221 tggcgcagct ggctcgcgaa aacgacgcca tggtgattgc cctcacctct gcgggtaccc
    14281 cgctcgcccg ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt
    14341 atatgcccat ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat
    14401 ttactttgcg acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga
    14461 aggaatcgcg ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt
    14521 cactgtccta atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat
    14581 ttcattcgga tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg
    14641 tccagaaggc ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat
    14701 aataatcttg aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac
    14761 ggctcgcctg aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg
    14821 gggcgtcatg tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt
    14881 aaagaaggca aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt
    14941 aaaggtgaag gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtt
    15001 gtgcctggcg acatcctgct gctggacgat ggtcgcgttc agttaaaagt actggaagtt
    15061 cagggcatga aagtgttcac cgaagtgacc gtcggtggtc ccctctccaa caataaaggt
    15121 atcaacaaac ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac
    15181 attaagactg cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgcggc
    15241 gaagatctga actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt
    15301 gccaaggttg aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc
    15361 gcctctgacg tggtaatggt tgcacgcggc gacctcggtg tggaaattgg cgacccggaa
    15421 ctggtcggca ttcagaaagc gttgatccgt cgtgcgcgtc agcttaaccg cgcggtaatc
    15481 acggcgaccc agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc
    15541 atggacgtag caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact
    15601 gccgctgggc agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg
    15661 gaaaaaatcc cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg
    15721 gaagaagcta ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg
    15781 attattacca tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt
    15841 ctaccaattt tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt
    15901 ggcgttacgc cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg
    15961 gttaatctgc tacgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag
    16021 ggcgacgtga tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa
    16081 tctcacctgc cccggccgct gatgaccggg gtatttagca acacataaat taggactact
    16141 cattcaatcc aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg
    16201 agatctaaat agcttgcaag acgtcgcata cccgcttcct ctgcaacact atatccaata
    16261 actgccatcg gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa
    16321 tcgttaatcg taatataata atcagcctga tattccatat attgtattgg atcacaaaaa
    16381 cagcctgccc caatcccaat agttctaact attctggctc tatctccata aaattggatc
    16441 ccaggaagat tataaatttt caaatttttg gcaaaacgtt tggtaatatt aatggcactg
    16501 tcaggttcta tagcatatac atggtacata tcatcagaag caacaatatt tgtaacatcc
    16561 agttctaact gctgagccaa agccttagac attccgaacg taggctctcg atccatgaca
    16621 tcatggcaac gaatgattac caaattattt ttatgcaaaa actccttttt ttgctcaatt
    16681 atttttaaat aactgagttc accgcgcgta taatccatta tttttctggc ccatgaaaac
    16741 tcaagaacgt caccct
//
LOCUS       DAZGJW010000101        16562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZGJW010000101 DAZGJW010000000
VERSION     DAZGJW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.101"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            56..271
                     /gene="xisR"
                     /locus_tag="ACHKIK_003355"
     CDS             56..271
                     /gene="xisR"
                     /locus_tag="ACHKIK_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFO9084468.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            273..1508
                     /gene="intR"
                     /locus_tag="ACHKIK_003356"
     CDS             273..1508
                     /gene="intR"
                     /locus_tag="ACHKIK_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9084469.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEVTNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGVVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQVAMLNARLS"
     gene            1560..2495
                     /gene="ttcA"
                     /locus_tag="ACHKIK_003357"
     CDS             1560..2495
                     /gene="ttcA"
                     /locus_tag="ACHKIK_003357"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO9084470.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2624..3997)
                     /gene="dbpA"
                     /locus_tag="ACHKIK_003358"
     CDS             complement(2624..3997)
                     /gene="dbpA"
                     /locus_tag="ACHKIK_003358"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO9084471.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4027..4200)
                     /gene="ynaL"
                     /locus_tag="ACHKIK_003359"
     CDS             complement(4027..4200)
                     /gene="ynaL"
                     /locus_tag="ACHKIK_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO9084472.1"
                     /translation="MTTLIYLQIPVPEPIPSDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4475..5458)
                     /gene="zntB"
                     /locus_tag="ACHKIK_003360"
     CDS             complement(4475..5458)
                     /gene="zntB"
                     /locus_tag="ACHKIK_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO9084473.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5472..5519)
                     /gene="ydaN"
                     /locus_tag="ACHKIK_003361"
     CDS             complement(5472..5519)
                     /gene="ydaN"
                     /locus_tag="ACHKIK_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO9084474.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            5713..6945
                     /gene="dgcM"
                     /locus_tag="ACHKIK_003362"
     CDS             5713..6945
                     /gene="dgcM"
                     /locus_tag="ACHKIK_003362"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO9084475.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6966..7529)
                     /gene="smrA"
                     /locus_tag="ACHKIK_003363"
     CDS             complement(6966..7529)
                     /gene="smrA"
                     /locus_tag="ACHKIK_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO9084476.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(7859..8767)
                     /gene="abgR"
                     /locus_tag="ACHKIK_003364"
     CDS             complement(7859..8767)
                     /gene="abgR"
                     /locus_tag="ACHKIK_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9084477.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            8943..10253
                     /gene="abgA"
                     /locus_tag="ACHKIK_003365"
     CDS             8943..10253
                     /gene="abgA"
                     /locus_tag="ACHKIK_003365"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO9084478.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQHHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            10253..11698
                     /gene="abgB"
                     /locus_tag="ACHKIK_003366"
     CDS             10253..11698
                     /gene="abgB"
                     /locus_tag="ACHKIK_003366"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO9084479.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            11735..13261
                     /gene="abgT"
                     /locus_tag="ACHKIK_003367"
     CDS             11735..13261
                     /gene="abgT"
                     /locus_tag="ACHKIK_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO9084480.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            13272..13787
                     /gene="ogt"
                     /locus_tag="ACHKIK_003368"
     CDS             13272..13787
                     /gene="ogt"
                     /locus_tag="ACHKIK_003368"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO9084481.1"
                     /translation="MLRLLEEKIATPLGPLWVMCDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            13982..14734
                     /gene="fnr"
                     /locus_tag="ACHKIK_003369"
     CDS             13982..14734
                     /gene="fnr"
                     /locus_tag="ACHKIK_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO9084482.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            14886..15836
                     /gene="uspE"
                     /locus_tag="ACHKIK_003370"
     CDS             14886..15836
                     /gene="uspE"
                     /locus_tag="ACHKIK_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO9084483.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(15886..16143)
                     /gene="ynaJ"
                     /locus_tag="ACHKIK_003371"
     CDS             complement(15886..16143)
                     /gene="ynaJ"
                     /locus_tag="ACHKIK_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO9084484.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            16387..>16562
                     /locus_tag="ACHKIK_003372"
     CDS             16387..>16562
                     /locus_tag="ACHKIK_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFO9084485.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVG"
BASE COUNT         4292 a         4069 c         4074 g         4127 t
ORIGIN      
        1 atcaacatcg ggcgtcaatt taagtctaac attggcgcct gccagaggtg atgcgatggc
       61 acaagtaatc tttaatgaag agtggatggt tgaatacggc ctgatgcttc gcactggtct
      121 gggggccaga caaattgaag cataccgcca gaactgttgg gtggaaggct tccacttcaa
      181 acgagtatct cctttaggga agccagacag taagcgaggg attatctggt ataactatcc
      241 aaagataaat cagtttatca aagactcatg atatgtctaa attaccaaca ggtgtcgaga
      301 ttcgaggtaa atacattcgc atctggttca tgtttcgagg aaaacgatgt cgggaaacat
      361 tgaaaggctg ggaggttact aacagtaaca ttaaaaaagc cgggaattta agagcgttga
      421 tagttcatga aatcaattcc ggtgagtttg agtatttaag acgttttccc cagtccagca
      481 ctggggcaaa aatggtgaca acgagggtca taaaaacgtt cggggagctt tgtgatatct
      541 ggacaaaaat taaagaaaca gagttaacaa caaacacaat gaagaaaacg aaatcacaat
      601 taaaaacact caggataata atttgtgaga gtactccgat atcgcatatt cgttatagcg
      661 atatcttaaa ctaccggaat gaactgctgc atggagaaac gctttacctg gataatccaa
      721 gatccaacaa aaaaggaaga accgtgcgca cagttgataa ctatatcgcc ctgctctgtt
      781 cgttgttacg ttttgcgtat cagtcgggat ttatatcaac caaaccattt gaaggagtaa
      841 agaaattaca gagaaacaga ataaagcctg acccgttatc taaaacagaa ttcaatgcat
      901 taatggaaag tgaaaaagga cagagccaga acttgtggaa atttgccgta tactccgggc
      961 ttcgtcacgg ggaactggct gctctggcgt gggaggatgt ggatttcgag aagggagttg
     1021 tgaatgtcag aagaaacctg acgatactgg atatgttcgg tcccccaaaa acaaatgcgg
     1081 ggatccggac ggtaacacta ctgcagcctg ctcttgaagc actgaaggag caatacaaac
     1141 tgaccgggca tcatcgcaaa agcgaaatca ccttttatca tcgggagtac ggcagaaccg
     1201 aaaagcaaaa actgcatttt gttttcatgc ccagggtgtg taacggaaaa caaaaacctt
     1261 attactcggt aagcagtttg ggggcaaggt ggaatgcagc agtaaaacgt gctggtattc
     1321 gccgccgtaa tccgtaccat acgcggcata cttttgcctg ctggctgttg acggcaggag
     1381 cgaacccggc atttatagcc agccaaatgg ggcatgaaac tgcgcagatg gtgtatgaaa
     1441 tttacggtat gtggattgat gacatgaacg acgaacaggt agccatgttg aatgcgcggt
     1501 tatcatagtt gcaaagtttg cccccaattt gccccattta gtaccagaga actgaaataa
     1561 tgcaagaaaa tcaacaaatt acaaagaaag aacaatacaa cctgaacaaa ttacaaaaac
     1621 gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc
     1681 gcatcatggt ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca
     1741 atttgcagca aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc
     1801 aaccgggctt cccggaacac gttctgcccg agtatcttga aaagctgggc gttgagtaca
     1861 agattgttga agagaatact tacggtatcg tgaaagagaa gattccagag ggcaaaacca
     1921 cttgctcact gtgttctcgc cttcgtcgcg gtatccttta tcgtaccgca acggaactgg
     1981 gggcgacgaa gatcgcgttg ggtcaccatc gtgacgatat cctgcaaacg ttgttcttaa
     2041 atatgttcta cggcggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca
     2101 aacatatcgt tattcgtccg ctggcctact gccgcgagaa agatattcag cgatttgccg
     2161 atgcaaaagc gttcccgatt attccgtgca acctgtgcgg ttcacagcct aacctgcaac
     2221 gtcaggtgat tgctgacatg ttgcgtgact gggataaacg ttatccaggg cgtatcgaga
     2281 cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg
     2341 attttaaagg cattacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc
     2401 gcgaagagat cccactacaa ccggcgggct ggcagccaga agaagatgaa aatcagttgg
     2461 atgagttacg actgaatgtg gttgaagtga aataaccagg atagcgcccg atgcgcaagc
     2521 gtatcgggct actcttatgg aggccggata agacgcggcc agcgtcgcat ccggcaatcc
     2581 cgaataagat gtttactctt gcacccggca attcaacatt tcattatttt aataaccgca
     2641 cccggcacgt ttttccttta atcttcccgc cctgtaactg tttccatgct ttatgagcaa
     2701 cagcctgacg gaccgcgaca tagacatgcg ccggatgcac ggcgattttg ccaatatctg
     2761 cgccatcaag cccgatatct cctgtcagtg cacctaatac atcacccggg cgcattttgg
     2821 cttttttccc gccatcgata cacaacgttg ccatttctgc ttccagcggc acaatggaac
     2881 tattagctgg cggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg
     2941 cccgctgtgc ttcttccgga gcacagaaac tgatcgccag accgctattt cctgcacgag
     3001 ctgtacgacc gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca
     3061 ccagctcaag cgatttaata tccagaccac gcgcagcaac atcagtagca accagtacac
     3121 gggcgctacc gttagcaaaa cgtaccagag tctgatcgcg atcgcgttgc tccagatcgc
     3181 cgtgtaacga caatgcactt tgccctactt cattcagcgc gtcgcagaca gcctggcaat
     3241 cttttttggt attgcaaaac accacgcaag aggatggctg atgcaagctt aataaccgtt
     3301 gcaacagagg gattttgcct ttgctggatg tctcataaaa ttgttgttca atgggtggca
     3361 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgtactcgt ccgctgattg
     3421 cggcgatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     3481 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     3541 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     3601 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg caacgaatca cgctgcatac
     3661 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     3721 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     3781 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     3841 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     3901 cggcggcctg caccggcgtc atggttaaat aacccaactc attaaggttc gtgagttggg
     3961 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     4021 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     4081 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     4141 aacaggatcg ctaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     4201 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     4261 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     4321 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     4381 atggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     4441 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     4501 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     4561 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taaccctgtc agaaaggtac
     4621 tgggtaaaaa gaccattgcc atcaatgaca ttgtataggt acgacgagct aaattttcct
     4681 gcatcacctg agcgatttca tccgccatca caccagtccg tgctatacag gcgtcgattt
     4741 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     4801 tcatccacgg caaacgttca ctggcaagac gagcatagac atcacgttgc ggtgccatat
     4861 agcgacgcat cacgattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     4921 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     4981 cgctggaatg atcggtcaac gcatcgcaca catccaccag ccaccctccg caatcggtcg
     5041 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     5101 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
     5161 gttcatcggt gctgccgttt atacagcgca atgtaataag cgtgccttca ccgagacggc
     5221 tgactcgggg ccgtgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
     5281 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
     5341 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
     5401 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag
     5461 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
     5521 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
     5581 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
     5641 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
     5701 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
     5761 tggattgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
     5821 atgcaagacc tcacgtttag tcagctacga accggaccct attccgtctc ctcacaaaaa
     5881 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
     5941 gttcagcgta aagaagagga aacagcatta agctgtcggc ttgttttgcg agagctgaca
     6001 gaaacagaac cggtgattat tttcgaaggt atcgaagcgc cggcaacgct gggtttaaaa
     6061 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
     6121 actaactctg caccaatgtt gttgattgac ccctcacgag atggccaaat cgtcgatgct
     6181 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
     6241 tgggaaatta atatgctcgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
     6301 cccggcggtc ataaaccttt gaattttgtt cataaactgg cggatggatc tactcgtcat
     6361 gtgcagacct atgccgggcc gattgaaatt tatggcgaca agctcatgtt atgtattgtg
     6421 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc tcaccatgac
     6481 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttaccgagcc aggccaaatg
     6541 cagcacctcg ccatcgccca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
     6601 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
     6661 accctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
     6721 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgt
     6781 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgtt ttaccgtcag cattggtgtg
     6841 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
     6901 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
     6961 cgagatcaac ggctgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
     7021 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc ggtgcaatat
     7081 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
     7141 tgcgatttat catcccgtcc tttgccatga ataatcagca cgttacgcaa accatcagcc
     7201 atggcttgtt gaatgaaact gaacatcatt ttgcggcact cttccaccgg ctggcgcaaa
     7261 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgt
     7321 tgcaaccctt cccgccgaaa ctccagcggc tgacttagtg ggatgatgtc gagaaatccg
     7381 gtggtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
     7441 gttggatgcc agtggacatc ggtagcacgt ttcagcggct ggacatcttc catggcgtca
     7501 agaaacagcg atttgtcgtc aaggttcatg ttacatcctc ccgcaattaa gagcgcgata
     7561 tgataaccag accgggtcgg tccaacaacg tcttacccaa aattccagta ataagttcca
     7621 aatattgccg atattttaag caaaatgctt atgcatgatt attcattcac gatattaata
     7681 atgtaactta tattttcgtg aaatctgtca ctgaagaaaa ttggcaacta aaggttaaaa
     7741 ccgttataac acagtcaccg gcgcagagga gacaatgccg gacttaagac gcggatgcac
     7801 tgctgtgtgt actgtagagt ctggcggatg tcgacagact ctattttttt atgcagtttt
     7861 aactttgcag atagccgcat tctcgccgga attgcgtgat taatgacgcg gtcagtggtg
     7921 tctggcgact atcacgccgc tgaatcaaat aataggccgc tttcggtaaa atttcgctaa
     7981 ccggcaacat caccagcccc tgtccgtgca agggatcgca gcccatttct tcaggcagta
     8041 tgctgagaaa atcgcttttt gccaccagac tgatacaggc tgagaacgtc tcgcagacta
     8101 caccgacctg tggcgtttgc gcctgatcgt caagcaattc actcaactgt ttgtagtaac
     8161 tgccgtgtgg cgtcggcatt gtccagctgt aatccagtaa ctgtttgatc gaacgggcac
     8221 caatggcggg gtgtcccggg cggcaaaaga tcgcgaattg cttttccagt aatttctcaa
     8281 aagtaaattc gtggtcgtac ggtccctgat aataggtatt gatggtgaaa tccaattctc
     8341 cctgacgcaa ttcattaatc atcgacacca gttgcccttc cataatgcgt acttttacct
     8401 gcggatgctg ctgatgaaaa cgagatatga cagctggcat cagactgcgg gaaatactgg
     8461 cccccatgcc gatattaatc tgccctgcca gttgcccttg tcgttggcga atttcctctt
     8521 gcgctgcgcg cagctcttca agaataagac tggcgtgctg ataaaaactt tcaccggcat
     8581 cagttaacgt cacgccttta ctacggcgaa aaaagagttg cgccgctaat ccttcttcca
     8641 gctcctgaat agatttactc agtgccggtt gcgacatatt caacattcgg ctcgctccgc
     8701 gaatgctgcc ctgacgagcc acttcaacaa aagcccgaat ttgatgaatt tttacctgaa
     8761 aagccatgac gccaccgata accgttattt atcagaccaa agaaactggc atctacttta
     8821 atgcagatga ttgtgtcagg gtaatttatg aacggttaaa actgtgaaaa atcagttagt
     8881 gataagtaaa aactatcgct acgtgaaccg ggtcacactt tttactgatg acgggaaagg
     8941 ttatggagtc tttgaatcaa tttgttaatt cgcttgcccc aaaattatcg cactggcgac
     9001 gtgattttca tcactatgca gagtctggct gggtggaatt ccgcactgcc acccttgttg
     9061 cggaagaatt gcagcagctc ggctattcac tggcgctggg ccgcgaagta gttaatgaaa
     9121 gtagccggat gggactacct gatgaactca ctctacaacg cgaattcgag cgcgctcgtc
     9181 aacagggtgc gctagcacaa tggattgcgg tttttgaagg tggtttcacg ggtatcgtcg
     9241 ccaccctgga taccggtcgc cccggtccgg ttatggcttt ccgtgtcgat atggacgcgc
     9301 tggatctcag tgaagagcag gatgtcagcc atcgccccta ccgcgacggt tttgcgtcat
     9361 gtaacgccgg aatgatgcat gcctgtggtc atgatgggca taccgccatt gggcttgggc
     9421 tggcgcatac ccttaaacaa ttcgagtccg gactacatgg cgtcatcaaa ctgatttttc
     9481 agcctgcaga ggaaggtacg cgtggcgcgc gggcgatggt cgatgcaggt gtcgtagatg
     9541 atgttgatta ttttactgcc gtgcacattg gcactggcgt acctgcgggc accgtggtgt
     9601 gcggcagtga taattttatg gcaaccacca aatttgacgc gcacttcacc gggaccgccg
     9661 ctcacgcagg cgcaaaacca gaagacggtc acaatgcctt gttggcggca gcacaagcaa
     9721 ctcttgcact gcatgcaatc gccccgcaca gcgaaggagc ttccagagta aacgtgggcg
     9781 ttatgcaggc aggaagcggc cgtaacgttg ttcctgcctc ggcgttgctg aaagtggaaa
     9841 cacgcggggc cagcgacgtc attaatcaat atgtttttga ccgtgcacaa caagcgattc
     9901 agggcgcagc aaccatgtat ggtgtcggcg ttgaaactcg cctgatgggt gcagctaccg
     9961 ccagttctcc ttcgccgcaa tgggtcgcat ggttgcaaag tcaggcggct caggtcgcgg
    10021 gggtcaatca ggccattgaa cgtgttgaag cgcctgcggg ttccgaagat gccacattaa
    10081 tgatggcccg cgtgcagcac catcaagggc aagcctccta cgtggtgttt ggcacacagc
    10141 tggcggcagg tcatcacaac gaaaaattcg attttgacga gcaggttctc gctattgccg
    10201 tcgaaacgct ggcgcgcacc gcgctcaatt ttccctggac gcgaggtatc tgatgcagga
    10261 aatctatcgt tttatcgacg atgcgattga agccgatcgc caacgttata ccgatattgc
    10321 cgatcaaatc tgggatcatc cagaaacacg ttttgaagag ttctggtcag cggagcatct
    10381 ggcttcggcg ctggaatctg caagcttcac cgttacccgc aacgtaggca atatcccaaa
    10441 tgcatttatt gcttcgtttg gtcaaggcaa accggttatc gccctgctgg gagaatatga
    10501 cgccctggca ggtttaagtc agcaagctgg ttgcgcgcaa cctacatccg cgacgcccgg
    10561 tgaaaatggt cacggttgcg gacacaattt gctgggaacc gccgcctttg ccgctgcaat
    10621 agccgtcaag aaatggctgg aacaatatgg gcaaggcggc acagtgcgct tttatggttg
    10681 tcctggcgaa gaaggcggct cgggtaaaac gttcatggtc cgcgaggggg tatttgatga
    10741 tgtggatgcg gcactcacct ggcacccgga agcctttgcc ggcatgttca atacccgcac
    10801 gctggcaaac attcaggcat catggtgctt taaagggatc gcagcacatg ccgcgaattc
    10861 ccctcatttg ggacgcagcg cccttgatgc cgtaacgttg atgaccactg gcaccaactt
    10921 cctcaacgaa catattattg aaaaagcgcg cgtacactat gccatcacaa atagcggcgg
    10981 gatctcgccc aacgtggtcc aggcgcaggc agaagtgctt tatcttatcc gcgcccccga
    11041 aatgaccgac gtgcagcata tttatgatcg ggtcgccaaa atcgccgaag gtgcggcatt
    11101 gatgaccgaa accacggttg aatgccgctt cgacaaagcc tgttccagtt atctcccgaa
    11161 tcgcacctta gaaaatgcca tgtaccatgc cctatcccat tttggtaccc cggaatggaa
    11221 ctccgaagaa ctggcttttg cgaaacaaat tcaggctacg ctcaccccca acgatcggca
    11281 aaacagtctg aataatatcg ccgcaaccgg tggcgaaaac ggcaaggctt ttgcactacg
    11341 tcatcgtgaa acggtactgg cgaatgaggt cgctccatat gccgccaccg ataacgtgct
    11401 tgcggcatcg actgatgtcg gcgacgtcag ttggaaactg cctgttgccc agtgtttcag
    11461 cccctgtttt gccgtcggta caccgctaca tacgtggcaa ctggttagcc aggggcgaac
    11521 atctattgct cataaaggaa tgctgctggc ggcgaaaact atggcagcaa ccacagtcaa
    11581 tctcttcatt gattcagggc tattgcaaga atgccaacaa gagcatcagc aagttacgga
    11641 cacgcaaccg tatcactgcc ctatcccgaa aaacgtgaca ccgtcacctt taaaataaca
    11701 acaacaacgc aaacacaaca accgaggaat gcccatgagt atgtcatcca taccgtcgtc
    11761 ctcccaatcc gggaagctct atggctgggt cgaaagaatt ggtaacaagg ttccccatcc
    11821 ttttttgctc tttatctatt tgataatcgt actcatggtg acgacggcaa ttttgtcagc
    11881 ctttggcgtc agtgcgaaaa acccgaccga tggtacgccg gtggtggtga aaaacctgct
    11941 cagtgtggaa ggattacact ggtttttacc caatgtgatt aaaaacttta gcggttttgc
    12001 tccacttgga gcgatcctgg cgctggtttt aggtgccggc ctagcggagc gcgtcggctt
    12061 actgccagcg ctaatggtta aaatggcatc gcatgttaat gcccgctacg ccagttatat
    12121 ggtgctgttt attgcttttt tcagccacat ttcttccgat gcggcgttag tgatcatgcc
    12181 accgatgggt gcgctgattt ttctggcggt gggcaggcat ccagttgcag gtttactggc
    12241 tgccattgca ggcgtaggtt gcggctttac ggctaattta ctgattgtca caaccgacgt
    12301 gttgctgtcg gggatcagca cggaagcggc ggctgcgttc aatccgcaaa tgcacgtcag
    12361 tgtaattgat aactggtatt ttatggccag ctccgtagtc gtactgacga ttgttggcgg
    12421 cttgataacc gacaaaatca tcgagccacg gttaggtcaa tggcagggaa acagcgatga
    12481 gaaactgcag acattgaccg aaagtcagcg ttttggttta cgcatagcag gtgtcgtatc
    12541 gctacttttt attgctgcga tcgcgctgat ggtgatcccg gaaaacggga tattgcgcga
    12601 tccgattaat cacaccgtga tgccatcacc ctttattaaa ggtatcgtgc cactaatcat
    12661 tctttttttc tttgtggttt cgctggctta tggcatcgct acccgcacaa ttcgacgtca
    12721 ggcggattta ccgcatttaa tgattgaacc gatgaaagag atggcgggat ttatcgtgat
    12781 ggtttttccc ctcgcccagt ttgtcgccat gtttaactgg agcaacatgg ggaaattcat
    12841 cgccgtgggg ctgaccgata tacttgaaag ttcagggctt agcggcatcc cggcgtttgt
    12901 cggtctggcg ttgctttcct ctttcttatg catgtttatt gccagcggtt ccgcaatctg
    12961 gtcgattctg gcccccattt tcgtaccaat gtttatgcta cttggctttc acccggcatt
    13021 tgcgcaaatc ctctttcgta ttgccgactc atccgtattg cctttagcgc cggtatctcc
    13081 ttttgttcca ctgtttcttg gattcctgca acgctacaaa ccagacgcga aactgggtac
    13141 ttactattcg ttagtcttgc cctatccgct tatctttttg gtggtatggc tgctgatgtt
    13201 gctggcgtgg tatcttgttg gtctgccgat aggtccgggg atttacccac gtttgtctta
    13261 agagagaacg gatgctgaga ttacttgaag aaaaaattgc cacgccactg ggtccactgt
    13321 gggtgatgtg cgatgagcaa tttcgcctgc gggcggttga atgggaagag tacagcgaac
    13381 gcatggtgca gctgctggac atccattatc gtaaagaagg ctatgagcgc atttctgcca
    13441 ccaacccagg cggtttaagc gacaagcttc gtgattattt tgccggtaat cttagcatta
    13501 ttgatacgct tcccactgcc acagggggga cgccatttca gcgcgaagtc tggaaaactc
    13561 tacgcactat cccctgcggg caggtaatgc attacggcca actggctgaa caattgggcc
    13621 gccctggcgc ggcgcgtgcc gtaggtgcgg caaatggatc gaatcccatc agcatcgtcg
    13681 taccttgcca tcgggttatt ggtcgaaacg gcaccatgac cggatatgca ggcggagttc
    13741 agcgaaaaga gtggttattg cgccacgaag gttatctttt gctgtaaaca ttaaacaatt
    13801 tgtgccagct tgttcacact tttatgtaaa gttaccctta acaacttaag ggttttcaaa
    13861 tagatagaca tatatttaca tctaatatcg gaattctctg ctgttaaggt ttgcttagac
    13921 ttacttgctc cctaaaaaga tgttaaaatt gacaaatatc aattacggct tgagcagacc
    13981 tatgatcccg gaaaagcgaa ttatacggcg cattcagtct ggcggttgtg ctatccattg
    14041 ccaggattgc agcatcagcc agctttgcat cccgttcaca ctcaacgaac atgagcttga
    14101 tcagcttgat aatatcattg agcggaagaa gcctattcag aaaggccaga cgctgtttaa
    14161 ggctggtgat gaacttaaat cgctttatgc catccgctcc ggtacgatta aaagttatac
    14221 catcactgag caaggcgacg agcaaatcac tggtttccat ttagctggcg acctggtggg
    14281 atttgacgcc atcggcagcg gccatcaccc gagcttcgcg caggcgctgg aaacctcgat
    14341 ggtatgtgaa atcccgttcg aaacgctgga cgatttgtcc ggtaaaatgc cgaatctgcg
    14401 tcagcagatg atgcgtctga tgagcggtga aatcaaaggc gatcaggaca tgatcttgct
    14461 gttgtcgaag aaaaatgccg aggaacgtct ggctgcattc atctacaacc tgtcccgtcg
    14521 ttttgcccaa cgcggcttct cccctcgtga attccgcctg acgatgactc gtggcgatat
    14581 cggtaactat ctgggcctga cggtagaaac catcagccgt ctgctgggtc gcttccagaa
    14641 aagcggtatg ctggcagtca aaggtaaata catcaccatc gaaaataacg atgcgctggc
    14701 ccagcttgct ggtcatacgc gtaacgttgc ctgatttttc cgcataactc actatccttc
    14761 tgtcatatca ttaaattttt ctgatttatt gatctggcag aaggttcatc actgtttcat
    14821 tcaccagata tgggttaatc ttttaattac aaactgcgtt gacagttgtt gtaaggagac
    14881 cctgtatggc tatgtatcag aacatgctcg ttgttatcga tcctaaccag gacgaccaac
    14941 cagcattgcg gcgagctgtt tatttacatc aacggattgg tggcaaaatt aaagcctttt
    15001 tgccgatcta tgacttctca tacgaaatga ccaccctgct ctccccggac gaacgtaccg
    15061 ctatgcgtca gggcgtcatc agccagcgta cagcctggat ccacgagcag gcaaaatatt
    15121 atctcaatgc tggcgttccc attgaaatta aagtggtctg gcataaccgt cccttcgaag
    15181 ccatcattca ggaagtgatc agcggcgggc acgatttggt gctaaaaatg gcgcaccaac
    15241 atgaccgtct ggaagcggtg atttttacgc caacggactg gcatctgtta cgcaaatgcc
    15301 caagcccggt gtggatggtg aaagaccagc cgtggccgga aggaggcaag gcgctggtgg
    15361 cggtgaatct cgccagtgaa gagccgtatc ataatgcgct caatgaaaaa ctggtcaaag
    15421 agacgatcga actggcagaa caagtcaacc ataccgaagt tcatctggtt ggcgcttatc
    15481 cggtaacgcc aattaatatc gcgattgaac tgccggaatt tgatccgagc gtttataacg
    15541 atgccattcg tgggcaacat ttgctggcaa tgaaagctct gcggcagaaa ttcggcatta
    15601 atgaaaacat gacgcacgta gaaaaaggtc tgccagaaga ggtgattcct gatttggcgg
    15661 agcacttaca ggcggggatt gtggttctgg gcacggtagg acgcaccggt atttcagcag
    15721 cattcctcgg caacacggcg gaacaggtga ttgatcatct tcgctgcgat ctgttggtca
    15781 ttaaacctga ccagtatcag acacccgttg aactggatga cgaagaagac gattaacgac
    15841 tcttcaataa ctgacaaggc ccgagttatc gggcctattt tttacttact gctcttcgcc
    15901 gccaaggaaa taaattccca acgggattgc cagcaacaca gtaaagacca ggctataaac
    15961 aaaaatcatc gatgactgaa tcacatacat tgatgtcgtc cactcagaca gcggaatatt
    16021 gtactgttct ataacgccgc cgatagtcgc gcgagtcaca accgacgccg caataatgaa
    16081 aaccgccaac aaaccaaaga gaaatttctt ccctttcgct gacttcagtt tcgccataat
    16141 catgatgtaa tccttgtgca gacaaatttc ttgttaccta aacgtaacaa taccatgaca
    16201 tttcaccgtt gtctgctctg aaaacctcac ctcttagctg gatttacaat caacgcattg
    16261 attagttagc taatgttgct acttccttcc ctgactattg atgatctgaa tgaattgtcg
    16321 cggtaaaata gccccgccag gggtttccgg catgacgaca attatctgga ggagcctgat
    16381 aaaacaatga tcgctgaact gtttacaaat aatgcgctta atctggtcat tattttcggt
    16441 agctgcgcag cattgattct gatgagcttt tggtttcgcc gcggaaatcg taaaagaaaa
    16501 ggttttttat tccatgcggt gcaattttta atctacacca taattatcag tgctgttggt
    16561 ag
//
LOCUS       DAZGJW010000102        16515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZGJW010000102 DAZGJW010000000
VERSION     DAZGJW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.102"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(77..229)
                     /locus_tag="ACHKIK_003373"
     CDS             complement(77..229)
                     /locus_tag="ACHKIK_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9084486.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(494..1228)
                     /gene="cysH"
                     /locus_tag="ACHKIK_003374"
     CDS             complement(494..1228)
                     /gene="cysH"
                     /locus_tag="ACHKIK_003374"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO9084487.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1302..3014)
                     /gene="cysI"
                     /locus_tag="ACHKIK_003375"
     CDS             complement(1302..3014)
                     /gene="cysI"
                     /locus_tag="ACHKIK_003375"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO9084488.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDHEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3014..4813)
                     /gene="cysJ"
                     /locus_tag="ACHKIK_003376"
     CDS             complement(3014..4813)
                     /gene="cysJ"
                     /locus_tag="ACHKIK_003376"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO9084489.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5132..5494
                     /gene="queD"
                     /locus_tag="ACHKIK_003377"
     CDS             5132..5494
                     /gene="queD"
                     /locus_tag="ACHKIK_003377"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO9084490.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5572..6843
                     /gene="ygcN"
                     /locus_tag="ACHKIK_003378"
     CDS             5572..6843
                     /gene="ygcN"
                     /locus_tag="ACHKIK_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO9084491.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6834..7094
                     /gene="ygcO"
                     /locus_tag="ACHKIK_003379"
     CDS             6834..7094
                     /gene="ygcO"
                     /locus_tag="ACHKIK_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO9084492.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCCLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7111..7686
                     /gene="ygcP"
                     /locus_tag="ACHKIK_003380"
     CDS             7111..7686
                     /gene="ygcP"
                     /locus_tag="ACHKIK_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO9084493.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7834..8694)
                     /gene="ygcQ"
                     /locus_tag="ACHKIK_003381"
     CDS             complement(7834..8694)
                     /gene="ygcQ"
                     /locus_tag="ACHKIK_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO9084494.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAVKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8691..9470)
                     /gene="ygcR"
                     /locus_tag="ACHKIK_003382"
     CDS             complement(8691..9470)
                     /gene="ygcR"
                     /locus_tag="ACHKIK_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO9084495.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRIEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9448..10857)
                     /gene="ygcS"
                     /locus_tag="ACHKIK_003383"
     CDS             complement(9448..10857)
                     /gene="ygcS"
                     /locus_tag="ACHKIK_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9084496.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10879..12333)
                     /gene="ygcU"
                     /locus_tag="ACHKIK_003384"
     CDS             complement(10879..12333)
                     /gene="ygcU"
                     /locus_tag="ACHKIK_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO9084497.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPQEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGTDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12403..13188)
                     /gene="ygcW"
                     /locus_tag="ACHKIK_003385"
                     /pseudo
     CDS             complement(12403..13188)
                     /gene="ygcW"
                     /locus_tag="ACHKIK_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13506..14783
                     /gene="yqcE"
                     /locus_tag="ACHKIK_003386"
     CDS             13506..14783
                     /gene="yqcE"
                     /locus_tag="ACHKIK_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO9084498.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEVRTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14810..16288
                     /gene="ygcE"
                     /locus_tag="ACHKIK_003387"
     CDS             14810..16288
                     /gene="ygcE"
                     /locus_tag="ACHKIK_003387"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO9084499.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGSVVCEGKGLLQPMHTPYAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSR
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         3921 a         4341 c         4172 g         4081 t
ORIGIN      
        1 atataaattt taaaaaaggg tgcaggagac caaaccatca caattcagta cgcggggttt
       61 tacccccgcg tggtggctac ttcttcgatt cgtaagcgag aactgcctta aactccatac
      121 cacgttctct gatactcaac tcacacaacg agtcacctac catcagcgtg aatcccagta
      181 ctgtacaaca cagtacgatg attgccacaa gggcatattt tgtcagcatg tcttgcctcc
      241 ctgcaaagaa gagactaaca ttcgaattgt ttgggttcga acgtaagcct caggttgata
      301 gaaatatcgc ctggggcttt tgtccatctg gaacctcgcg aatgcttaac gccagacagc
      361 ctcaagcacc cgacgccatt ctatacctga taattcttcc agcggcgttc acgtttaaaa
      421 tcaggaatac tgctcgcaaa ataaaaacgc ccccatcatt tctgacagag gcgtttaatt
      481 tatccggcaa tatttaccct tcgtgcaatc cacattccct ttttaagcca aagaaacgtg
      541 tttcttcttc cgacatgcca ggttcccatt tacgggttgt atgggtatcg cccaccgata
      601 aatatccttc gtcccataat gggtgatatt tcaggccatg tttttgcaga tactgatata
      661 tcgttcggtt atcccagtcg ataatcggca gcactttaaa tacgccacgc tgaattgcca
      721 gcaccggtaa attggcgcgg ctgccggatt gttcgcggcg caggccagca aaccacgttt
      781 gcgcattcag ttctttcagc gcccggttca tcggttcgac tttgttgatg tcattgtact
      841 tttcaatgcc ttcaacgccc tgttcccaca gtttgccgta gcgtgcttcc tgccaggcgg
      901 cgctttcggt agcgcggtac actttcaggt tgagcttgag tttgtccgtt aactcgtcaa
      961 taaagcggta ggtttccggg aacagatagc cggtgtcggt gaggatcacc ggaatatccg
     1021 ggtgaatctg attcaccaga tgcaggctca ccgccgcctg aatgccaaag ctggaagaaa
     1081 gtacatattc accgggcaga ttatccagcg cccaggcaac gcggccttca gcgtccagtt
     1141 tttccagttc ggcgttagtt tctgccagcg ccagaatgcg atctaccttc ggcagttcgt
     1201 tcagggcgtt tagatcgagt ttggacatag attcctcact gtttgccttg cctgatgcga
     1261 cgctcacgcg tcttatcagg cctacaagac cgggctgatg gttaatccca caaatcgcgc
     1321 gccggatcga gcaccgggcg aatgatgccc gcacgcaccg taaagtcgcc gaagccttca
     1381 cccgcttcgc gctctttcgc ccagcgccct atcagttcat caagcgacgc caggatttcc
     1441 ggctcggtga tgttttcttt atacatccgt gggatacgtg tcccaatgcg gttgccgcca
     1501 agatgcaggt tgtagcgacc cggcgcttta cccaccaggc ccacttccgc cagcatcgcg
     1561 cgaccacaac cgttcgggca gcctgttaca cgcatcacga tatgctcatc gctgacacca
     1621 tgtttcgcca ttaaattatc gatgttgtcg ataaaagacg gcaggaaacg ctctgcttcc
     1681 gccatcgcca gcgggcaagt cgggaacgag acgcaggcca tcgagttttc acgctgcggc
     1741 gtgacggcat tcattaaccc gctctctttg gcgatcttct cgatcttcgc tttctcgctt
     1801 tccggtacac cggcgatgat cagattctgg ttcgccgtaa tgcggaaatc gcctttgtgg
     1861 atcttcgcga tctccagcag gccggttttc agcggacgcc ccggataatc aaggatgcga
     1921 ccattttcga taaacagcgt caggtgccag ttatcatcaa tgcccttaac ccagccaata
     1981 cgatcgcctc gtccggtgaa ctcatatgga cggatcggtt caaatttgat ccccgcgcga
     2041 cgctccactt ccgctttaaa cgtctcaacc cccacgcgct ccagcgtgta tttggttttg
     2101 gcatttttac gatcggttcg gttaccccag tcacgctgag ttgtcacgac ggcttcggcc
     2161 accgccagcg tatgctccag cggcagatag ccaaactcgc tcgccgtgcg ggcgtaggtt
     2221 ttcttgttgc cgtgttcgat agaaagccca ccgcccacca gcaggttgaa gcctaccagc
     2281 ttgccgtttt cggcgattgc cacgaagttc atgtcgttgg catgcagatc gatatcgttc
     2341 tgcggcggga tcactaccgt ggttttgaac ttacgcggca ggtaggtctg accaaggatc
     2401 ggttcttcat cagtagtggc gactttttcg tggtcgagcc agatctccgc atacgcgcgg
     2461 gtacgaggca acagatgctc agaaatcttc ttcgcccact cgtacgcttc cgcgtgcagc
     2521 tgcgactcgt aagggttcga ggtgcagagt acgttacggt tcatgtcgtt agctgtcgcc
     2581 agcgcatcaa gaccgaccga gtgcagcatc tggtgcaccg gtttgacgtt ctttttcaga
     2641 atgccgtgga actgaaacgt ctggcggttg gtcaggcgaa tgctgccata gatggtgttt
     2701 tcaccggcaa atttgtcgat cgcctgccac tgtttagtgg taatcacccc acccggcaga
     2761 cgacagcgaa gcagcatcgc gtggcgcggc tccagcttct gttcagcacg ttcggcgcgg
     2821 atgtcgcggt catcctgctg atacatgccg tggaagcgga tcagcaggaa gttgtcgccc
     2881 ttaaagccgc cggtcagacc gtcgtttaaa tcttccgcaa tggtgccgcg caggtagttg
     2941 ctttcaagct tcatgcgctc ggcgtctgtc agttttcctt cgaccactaa aggccctgga
     3001 tgtttttcgc tcattagtag acatctcgct gataacggcg ctctacgcgc agctcactta
     3061 aaaattcatc cgccgcttcg gtgtccatgc caccaaattc ggcaatcact tccagaagtg
     3121 cctgctcaac gtctttcgcc atgcgattag cgtcgccgca gacataaatg tgggcaccat
     3181 cattgatcca gcgccacagc tccgcgccct gttcgcgcag tttgtcttgt acgtaaactt
     3241 tttctttttg gtcgcgcgac caggcaagat cgatacgtgt cagcacgcca tctttgacgt
     3301 agcgctgcca ctccacctga tacaggaagt cttccgtaaa gtgcggatta ccaaagaaca
     3361 gccagttttt acctggcgct tcgtcggcgg cgcgttgctg cataaaggcg cggaacggcg
     3421 caataccggt gcctgggcca atcataatca ccggggtttc cgggttagcg ggcaggcgaa
     3481 agttatcgtt atgttcgata aatacgcgga cttcgccctc ttcttccacg cgatccgcga
     3541 ggaagctgga ggcaccaccg gcacgggctc ggccttccac gtcgtaacgc accacaccaa
     3601 cggtgacgtg tacttcgttc tcgacttccg cctgcgagga ggcgatggaa tacaggcgcg
     3661 gggtcagcgg gcgcagcaga ttaatcagcg cttcggcatc cagttgcgcc ggagagaaac
     3721 gcaccatgtc gacaatcggc gtcgtcgcgg cgtaatgctg taacttcgct ttatcgccca
     3781 ccagcggcag cagtgtttcg ctgcgggtaa gcgtggcgta attctcaaca atgttggcgg
     3841 tgttgacggt cagttcgaag tgccactgta gcgcttcgtt cagaggcaac gttttgccct
     3901 cgacggtgac aggttcatcg cctttcagcc acagcagttc gacaagttct ttcaccagtg
     3961 ccggatcgtt ctgataccag acgcccagcg cgtcacccgg ctggtaacgc aggcccgagt
     4021 cacctaagtc aatttcgata tggcgaacgt ctttttcaga gttacgcccg gtaattttct
     4081 ggttaacaga gaggctagcc accagcggcg cgtctttgct gtacgggctg gtgtggattt
     4141 catttaccgc gccagtagcg acggattgcg aaggtgccgc gacaggcgca cgcgatttaa
     4201 gcgcatcaac cacgcgggcg cgccactcgc tggcagcagc ctggtattca acatcggcat
     4261 cgacacggtc gagcaggcgt tcaccaccca gttccgccag cttgctgtcg aaatctttcc
     4321 cggactggca gaaaaattca taagagctat cgccgaggct aaacacggca aacgcggtgt
     4381 tttccagctt tggcgctttt ttggagaaca ggaacttatg cagcgcgacg gcttcttccg
     4441 gcggttcccc ttccccttgc gttgacgtca ctacgatgag cagtttttcg ctggcgattt
     4501 gtttgaattt atagtcgccc gcgttcacca gcttaacgtt cagttttgct gctaataaat
     4561 catcgcgtaa tgcttcagca acccggcgcg cattgccggt ttgcgaggcg gagataatag
     4621 ttatacccgg catttctgcg gctggcgctg gcgtcgctgc aagcgcagca ggctgctgat
     4681 tgagcacgcc ccagaaatag ccagaaaccc aggcaagctg ggtgggagtt aaatcggtcg
     4741 tggccgcctg aaggcgtgcc agttgctccg ggttcaacgg aagcaacgcg gaaggtggga
     4801 cctgtgtcgt catgcgtcgt tatgttccag taagcaaagc tgtttctgcg ccctgtcagc
     4861 gcccataaaa cagaagagaa ggtaaggtta acggggtgaa cggtgtggat taaagacggg
     4921 atagcgataa ctaataacca aatcgactaa cctgttttag caatagtctt taacaacaaa
     4981 atagattaac caacctaatg aaaaacaaat gaatttagcc aatcattaag ataaatcagc
     5041 gattttgcgc aacaaagtcg ttttagataa tgcgaaaaaa cagcctttcc ggtactctac
     5101 ggcggtttta tcgtcctgta gagaaattat gatgtccacc acgttattta aagatttcac
     5161 cttcgaagcc gctcaccgct taccacacgt cccggaaggg cataaatgtg gtcgcctgca
     5221 cgggcattcc tttatggtgc gactggaaat taccggggaa gtcgatccgc atacgggctg
     5281 gattatcgat ttcgctgaac taaaagcggc gtttaaacca acctacgagc gcctcgatca
     5341 ccattatctc aatgatattc caggtctgga aaacccaacc agcgaggttt tagcaaaatg
     5401 gatttgggat caggttaaac ccgttgtgcc gctgttaagt gcggtgatgg taaaagaaac
     5461 ctgtaccgca ggttgtatct atcgcggcga atgataagag tgtgtcggcg gtcaatttcc
     5521 cttaagtaac gctatgttag ggtgttgtgt tctggatatc tggggcatga catggaagac
     5581 gactgcgaca ttattattat tggtgccggt attgcaggca ccgcttgcgc gttacgctgc
     5641 gcgcgagcgg gtttatccgt tttgttactg gaacgcgctg aaatccccgg cagcaaaaat
     5701 ctttccggcg ggcggttata tacccatgca ctcgcggaac tcctcccgca atttcatctg
     5761 accgcgcctc ttgaacgacg catcactcac gaaagccttt ccctgttaac gccggatggc
     5821 gcaacgacgt tttccagctt acagcccggc ggtgaatcct ggagtgtatt acgtgcacgg
     5881 ttcgatccgt ggctggttgc cgaagccgaa gaagaaggtg tcgaatgcat ccccggagcg
     5941 acggtggatg cactgtatga agaaaacggc agggtgtgtg gtgtcatttg tggtgacgat
     6001 attctccgtg cccgttatgt ggtgctggca gaaggtgcca acagcgtcct ggctgagcgt
     6061 cacgggttag tgactcgtcc tgctggtgaa gcgatggcgt tggggatcaa agaagtgctg
     6121 tcgctggaac catccgctat tgaagaacgt tttcatctgg agaataacga aggcgcagcg
     6181 ttgctgttca gcggcaggat ctgtgatgac ttacccggcg gcgcatttct ttatactaat
     6241 caacaaacgc tctcgttagg gattgtttgc ccgctctctt cccttacgca aagtcgtgtt
     6301 ccggcaagcg agctgctgac tcgctttaaa gcgcatccgg cagtgcgccc gcttatcaaa
     6361 aacacggaat cactggagta tggtgcgcat ctggtgccag aaggtggctt gcacagtatg
     6421 ccggtgcaat acgccggtaa cggctggctg ctggtgggcg atgcgttgcg cagttgcgtc
     6481 aataccggaa tttccgtgcg cggcatggat atggcgctga ctggcgcgca ggcggcggca
     6541 caaacgctga taagcgcctg ccagcaccgc gagccgcaaa atctgtttgc gctttatcat
     6601 cacaacgtag agcgcagcct gctatgggat gttctacaac gttatcagca tgttccggcg
     6661 cttttgcaac gccctggctg gtatcgggcg tggcctgcgt taatgcagga tatttcccgc
     6721 gatttatggg atcagggtga taaacctgtt ccaccgctgc gccagttatt ctggcgtcat
     6781 ttacgtcgtc atggcctgtg gcatctggcg ggcgatgtta tcaggagtct gcgatgtctg
     6841 tagcccataa tctctggcgc gttgctgatg cgccgcacat tgttccggct gactccgttg
     6901 agcgtcagat ggtacaacgg ttgattaacg cctgtccggc aggtcttttt tcgctcacac
     6961 cggaaggtga cttacgcgtt gattatcacg gctgcctgga gtgtggcacc tgctgtttgc
     7021 tgtgcgacga atccacacta caacagtggc gctatccgcc ttccggattc ggcatcacct
     7081 accgctttgg ataaataata aggataattt atgcccctct tacacctgct ccgccagaat
     7141 ccggtgattg ctgccgttaa agataatgcc agcctgcaac tggcaatcga ctctgaatgc
     7201 cagtttattt ccgtgttgta cggcaatatc tgcaccatca gtaatatcgt taaaaagatt
     7261 aagaacgccg ggaaatatgc ttttattcat gttgatttgc tggaaggcgc gtcaaataaa
     7321 gaagtcgtga ttcagttttt gaaactggtg accgaggcgg acggcattat cagtaccaaa
     7381 gcctcaatgt tgaaagcggc aagagcggaa ggttttttct gtattcatcg cctgtttatt
     7441 gttgattcaa tttcgtttca caacattgat aagcaagttg cgcaatcgaa tccggattgt
     7501 attgagatcc tgccaggctg tatgcccaaa gtgctgggct gggtgacaga gaaaatccgc
     7561 caaccgctga ttgccggtgg gctggtgtgc gatgaagaag atgcgcgtaa tgcgattaac
     7621 gcgggtgtcg tggcgctttc caccacgaat accggggtct ggacgttagc gaaaaaatta
     7681 ctttgacggg ataatcgtaa ccaattgaat ttggtttgat ttgtaggccg cacgccacat
     7741 ccgacattca gcgcctgatg cgacgcttga cgcgtcttat caggcctaca agtccccagg
     7801 ccagaaccgt aggcaggatc atccggcaac acgttattgg caatctgcgt gaatgtttgt
     7861 caccagtgcc tccagcacca ctttccagtc atcaaccacg ccaacatctg cctgtgaaaa
     7921 taccgcagcg ctggcgtcat gattaatcgc caccacaaac ttgctattgc gtacgcccgc
     7981 cattaacgct gctgcgccag aagcgccaac aacaatacac acctcaggtg ccagcagatg
     8041 tccggaaata ccaatcactt tttcagcatc aacgccaccg ttcatcactc gcgcccgact
     8101 gtagcccact tcggctccca gcttttctgc cagcatagcg atttcctggt tatctgcctc
     8161 accgccctgc cccaccacca gcacccgtct ggcctcagct aatgggtcac gggtaacatt
     8221 tttcagatcc tctgtgctaa ccagccagtc gggaagcgcg ccggggacaa tgttcagttg
     8281 ctgcatacca gagggcaacg tggcgttttt cactgctccc gcctgacgcg ccagggaaag
     8341 gcatagcggg cgtttttctg tttgcagcgt cgccgttagc gcatttcccc agtgggattt
     8401 gcgcacgctt acggttggaa tatctaacga agtcacctgg cagatgcttg caccatgtaa
     8461 acgccaggcc agccgggtcg acagttcatc gccaaacgtt cccggcggaa agagaacaac
     8521 gtccgcaggc gttcgctgcc actgttccac caacgcatcc agcacctgtt ccgccactac
     8581 aggctgaggt tcaatctgcc agtgcgccag ggtacaaccg ctgaaatcct gcgccgccag
     8641 ccagcttgct attgcggcat tctcctgatt tattgtcaca attgcgatat tcatggctgc
     8701 atcctttggc gtaaatagtc ctgccatagc ttttgagctt tttctgctac cgtttgccca
     8761 tcaatcagcg tcgctccgcg tcgttgctct gctcttgcca gttgcagaca ctgcatagct
     8821 ggcatttcgg cagcaacagt tttacggata atttctgctt tccccgcagc cattcgctgg
     8881 cgcattcccg gtactggcaa ggccacttca ccgcactgac gcacggcaat caccgcaggc
     8941 aagcgaaccc ggcaacaacg caaaccatgt tcaatacgct gttcgagggt gataaacagc
     9001 gcgtcgagag tgaaacgctc cacctgggtg aagcagggcc agcccagcat ttccgccagc
     9061 aaaaatggcg tttgcccatt ctgcccttcg ctgctttggc agccggtaat tatcagatcc
     9121 agcggattct gatgctgcca ttcggcaata tggcgagcga caaattccgg cgcaaagcgc
     9181 agatctgccg ccgtctccag caatacagct tcctcaaacc caagagccat gagatagcgc
     9241 agccagtgca atgcccgttc atcccccatg ctcaacgcgg ttaaagacat cggcgtaccg
     9301 ttttttctct gcgccaacag cagcgcggca gcggcctgtt catcagcacc gagtaaactt
     9361 cgcagtagcg aaatatccgg tccgctttta ccctgagccg ccgcctgcca ctctttttcc
     9421 gccagcattc cggcatccgg ttcggcttta aacgctaaca gaatgttcat tcgcacctcc
     9481 tacatttccc gccgccacca gcgggagggc tttagtttct ggtgcccata gccaggtcac
     9541 aacaaaacca accaacaaca ctgtcgccag gagcaataag gtgacttgca ttccccactg
     9601 cgccagcacc cacggcagca ggccagtact taccgccgcg ccaagtcgac tcatggcggt
     9661 ggcaaaaccg acgcccagcg agcgaatgtc agtaggaaaa ctttccgcag gcaaaatgcc
     9721 caccagatta ctgactgccg aaatggtggt gctgaagaga acaaaaagca gcagcgttaa
     9781 tgaactgccg gaaggcaaac aggccattac taccagcgtt gccgccagca gcaaaaaact
     9841 tcccagcaaa aatttgcgat gtgccagcag gtgcgtcaga actaatccca gcagcgcgcc
     9901 cacaattaac aacgcattaa gcatcaggct ggcagtcagc gcatcttcca gaccaatagt
     9961 ctgggcgata gttggcagcc aggtataaat cacaaaccat gggattacga ggcagacaaa
    10021 gaagacgctg ttaaacgccg tgcgccgcca gtaacgcgaa gagaacaagg ttttgatgtg
    10081 tttatgggtc gccgttacca cttcatcgcc cagtaaaaca tggggaccaa aatagcgatg
    10141 cacgatagcg tgagcttctg caaaacgccc ctggcgcagt agccagcgtg gtgattctgg
    10201 cgttccccag cgtaataacg taatcaacaa cgcgggcaga gctgccgatg ccagtagcca
    10261 gcgccaggcc tccgggtttt cggaaataaa gtgatgtccg gcaatacttg ccagcacata
    10321 gcctacggtc cacaccacgc tgaatgcgcc cagcaaaata ccgcgatggc ggcgcgggga
    10381 aaattcagcc agcaaggtgt gacctactga ataatcgcct cccagaccaa tgccaatcaa
    10441 aatgcgcagt ccaataagat gctctggcgt ggtggcaaaa aattgtaaaa acgaagcaag
    10501 cgtaatcagc aaaaagctga aggtgaagat tttttgccga ccaatatggt cggagatcca
    10561 cccaagaacc aggctgccaa ggaacaaacc aaggagcgcc gagccgccga tcattcccgc
    10621 cataaacggc gtcagttgca tggcgggcgt aagctgaata atggcgtaac caatgacgcc
    10681 gagaacataa ccgtcggtca ggtgtgcgcc gaaagtgagc gcagcaatgc ggcagtgaaa
    10741 acggttaagc ggtaaatcat ccattcgcac cggtgaagtg ttcatcttta ccccttcatt
    10801 gtccggtaat aagaacggct tctcgcctga gaagccgaaa aggcaacgcc ccgtcatcgc
    10861 ggaacgctgc ctgacacatt atttttcaat cggatagata gtacccgtat tcataatgcc
    10921 attaggatcg aactgctttt tcagcccttc cagcaacgcc cacgcgctgc cgtgttccag
    10981 tttgctccag tgaacgcgat gtttaccgat accgtggtgg tgcaccatcg aaccgccgag
    11041 gcgaatggtt tcttcacaga tgatcttgtt gagcggattg tggtacttgt cgatttcctc
    11101 ttccggctta cagtcaacga cgttgtaatc gtagacgaag tacatgttgg tgccgttctg
    11161 atagctatga gaggaatgac cgcccagcat ggtgatgtcg tcggcgtgcg ggaactcagt
    11221 acgaatacgg ttaataacgc tttcgtagat ttcgtggatg cagctccagc agccggacac
    11281 ttcagtggta aagcccatgt tgccggtttt gaggatctgc acacgttcgg cagccacttt
    11341 atccggtccc cagttcaggt tgttaaacca ggtttcgatc agtttgctgt ccacgcgctg
    11401 gcactgcggg tagcgggcaa cgatttccgc aatcccttcg cccgtcgcct tcgcaatgcg
    11461 agggttacct tcagccataa agatcagcac acattttccg tctgcaaaat gggtgaagtg
    11521 ttgggtgcca tcttcagcgt catacaaacg agcgatcgac ggacgatacc cttccaccat
    11581 gacttcacgc aggatgttga agccggtttt catgtcttcc aggatatagc cgtagaagag
    11641 gttgttttcc ggggtgaatt taaagatttt cactgttact tcagtgatat agcaaagtgc
    11701 accttcgtta ccgatgatga tgtggcgaat gtccgtgcca gccgcgcggc gtggcacgtt
    11761 tttaatgcgt gtgacggtgc catcagccaa tactgcttcc agaccaacga ccatatcttc
    11821 gattgcgccg tagagtgtgg agaactgccc gatactgcgg gttgctacca ggccgcccat
    11881 ctgcgccagc ggctttgact gcggagaatg ccccgtggtg taaccttttt cacgcaacgc
    11941 attttccagc acttcctggg gaacgccaca ttgcgccgtc gcctgcatat tctcaatatc
    12001 aatattaatg atttgattca tggcggaacc gtcgagcacc accgagtttt ctacaacagt
    12061 ttccagccca ccttcagtgg cggaagcgcc ggtacgcggc acaccgttaa ttttgtgcgc
    12121 attcataaaa ttcagcacac gggagacttg ttctgtggaa ccgagtttta cgaccgctgc
    12181 cggaatcggc aaagtataaa taccatgaat atccggaaat ttacgaaaac ggtcaatact
    12241 gttcttcttt aatactgttt catcggtaat tacgcgatct gcaccaacaa tttcctttag
    12301 ttggtcgaca atcgctgcgc gagataaaga catagtaatt ccttcctgat aataaaaatg
    12361 gaataatcat taataagaga aaacagaaac caccgttatg gattagcgca ctaaataacc
    12421 accatcgacc actaataaat gcccattgac ataatttgaa gccggacttg cgaggaatac
    12481 agctgcgccc attaaatcct gagtatcgcc ccaacggttt gccggaatat gatcaagaac
    12541 gcgctgattg gtttctggat tactgcgtgt cgccagcgta atatctgttg cataatagcc
    12601 aggggcaata ccatttacct gaatattata ttgacctagt tcatcacaat aagctttggt
    12661 gaatccggca agagcatgtt tagtggcaga atatgcaggt gaccattgtc cacctaagta
    12721 agagaacaat gaacagatat taataatttt gccgcttttc tgcgggatca taatttttgc
    12781 agcttcatag cttaactcga atgcggcggt caggttcaca tcaatcatcg gatcccagtc
    12841 ggcacgaccg aagtccagca ccttattcag cttacaaata ccggcattgt ttaccagaat
    12901 atcaactgta ccgaaacgct cacagcaagc agcgataatc ttctgcggcg cgccttctgc
    12961 ggtgatatcc acctgcatga agtccacctc aacaccctgt ttttcaatca tttcctttgt
    13021 ttcgccgtta tctttgacga aactaggaat aaagatattt gcgccagctt tggccaacgc
    13081 catggcaaat gcctagccta aaccgctatt cccaccggta acaattgcgg ttttaccttt
    13141 cagtgagaaa aaatccattg agaacgcatt gagagattcg attgacatag ttagctccaa
    13201 tttccgtaag gcaaaaaaaa gaaaggtaac ttcccccgcg agagggaagt tacctttctc
    13261 attatctctg gtaactggag ataattttta gcataacggc ataactgcta atgtgatcgt
    13321 aatcacagtg tgatattcgt attgtcaaat agttttaaaa agcagaagcg tgattactct
    13381 cacacaacac ctctgctttc catgttattt cttattgcaa ttactagaga cctgagaaaa
    13441 gtaatccacc tgaaagggcg gcttactttt ctcttttttt tgtctattac aaatgggaat
    13501 acgatatgca acacaactca tatcgccgtt ggataaccct cgcgataatt agttttagcg
    13561 gcggcgttag ttttgacctg gcttatttac gttatattta tcaaattccc atggcgaaat
    13621 ttatgggatt cagcaatacc gagataggtt taataatgag tacctttggt attgcggcta
    13681 ttattcttta tgcccccagc ggcgttattg ccgataaatt ttcacaccgc aaaatgatta
    13741 cttccgcgat gatcattacc ggattactgg gtctgttaat ggcaacgtat ccaccgctgt
    13801 gggtaatgct ctgtattcag gtcgcctttg cgataacgac gattttaatg ctgtggtcgg
    13861 tgtcgattaa agccgcctcg ttgctgggcg atcatagtga gcaagggaaa attatgggct
    13921 ggatggaagg gctgcgcggc gtcggtgtaa tgtcgctggc ggtgtttacc atgtgggtct
    13981 tttctcgctt tgcaccggat gacagcgcca gcctgaaaac agtcattatc atctacagtg
    14041 tggtttacat cttgttgggg attctgtgct ggttttttgt tagcgataac aacaacctgc
    14101 gcagtgccaa taacgaagaa aaacagtcat tccagcttag cgacatcctt gccgttttgc
    14161 gtatcagcac cacctggtat tgcagcatgg tgatttttgg tgtcttcaca atctacgcca
    14221 ttctgagtta ctccaccaac tatctgaccg aaatgtacgg catgtcgctg gtggcagcga
    14281 gctacatggg gattgtgatc aacaaaatct tccgcgcgct gtgcggccca cttggcggca
    14341 ttatcaccac ctacagtaaa gtgaaatccc ctacccgcgt gatccaaatc ctttccgtac
    14401 tcggcctgct ggcgttaact gccctgctcg tcacgaactc taacccgcaa tcggtcgcga
    14461 tggggattgg cctgatttta ctgctgggat tcacctgtta cgcctcacgc gggctgtact
    14521 gggcctgccc tggcgaagtg agaacaccgt cttacattat gggcaccacg gtaggtattt
    14581 gttcggtgat tggattcctg ccggatgtct tcgtttaccc gattatcggc cactggcaag
    14641 acactctgcc cgcagcagaa gcctaccgca atatgtggct gatgggaatg gcggcgcttg
    14701 ccatggtgat tgtctttacc tttttgctgt tccaaaaaat tcgtactgct gatagcgccc
    14761 ccgcaatggc tagcagcaag taagccagga aaattacagg agaaaaatca tgtcgaaaaa
    14821 atacatcata gggattgatg gcggaagtca gagcacaaaa gtggtgatgt acgatctgga
    14881 aggtagtgtg gtttgcgaag gtaaaggctt attacagccg atgcacacgc cttatgccga
    14941 tactgcagaa catcctgacg acgatttatg ggcatcatta tgttttgcag gtcacgattt
    15001 gatgagccag tttgccggga ataaagaaga tattgttggc atcggcctgg gctccatccg
    15061 ttgctgccgt gcgttattga aagccgatgg cacgcctgct gcgccgttga ttagctggca
    15121 ggatgcacgc gttacacgcc cttacgaaca tacgaatcct gacgtggcgt atgtcacctc
    15181 tttttcgggt tatctgacgc atcgcttaac cggcgagttt aaagacaata tcgccaacta
    15241 ttttggtcag tggccggtgg attataagag ctgggcatgg agcgaagatg ctgcggtaat
    15301 ggataagttt aatatccccc gtcatatgct gtttgatgtg caaatgcctg gcaccgtcct
    15361 cggacatatc acaccacaag ccgcactggc gacacatttc ccggcaggac tgccggttgt
    15421 ttgtaccacc agtgataaac cggtagaagc tctgggggcc ggattactgg atgatgaaac
    15481 tgcggtaatt tctcttggca cttacatcgc attgatgatg aacggcaaag cactgccgaa
    15541 agatccggtg gcgtactggc cgattatgtc ttctattccg caaacattgc tgtatgaagg
    15601 ttacggtatt cgcaaaggta tgtggacggt gagccggctg cgtgacatgt taggcgagtc
    15661 gttaattcag gatgccaggg cgcaggatct ttcaccggaa gatttactca acaagaaagc
    15721 ctctagtgtg ccacctggct gtaatgggct gatgacggtg ctggactggc tgaccaatcc
    15781 gtgggaaccg tacaaacgcg ggattatgat cggctttgat tccagcatgg attacgcatg
    15841 gatatatcgt tcgatattgg aaagcgtggc gctaacgctg aagaacaatt acgacaatat
    15901 gtgtaatgaa atgaatcact ttgcgaagca tgtgatcatt actggcggcg gttcgaacag
    15961 cgatctgttt atgcaaattt ttgccgacgt gttcaacctt ccggcacgcc gtaacgccat
    16021 taacggttgt gcaagtctgg gagcagcgat taatacagcg gtaggtctgg ggctataccc
    16081 ggattacgca acggctgttg ataagatggt tcgcgtgaaa gatatcttta taccgattga
    16141 gagcaatgcc aaacgctacg acgcgatgaa taaaggcatt ttcaaagacc taaccaaaca
    16201 tactgatgtg atcctgaaaa aatcgtatga agtgatgcat ggggaattgg ggaatgtgga
    16261 ttcgatccag agctggtcga atgcgtgaat gaggtaggcc gggcggaaaa ctgcccggcc
    16321 tgaacatacc taaatggtta tccccgctga cgcggggaac ataagttcga caacttaaaa
    16381 aatgataaca agtccggttt atccccgctg atgcagggaa cataataaaa actttaacta
    16441 gttcggttta tccctgtttt tacaaggaat attaactaca ctcgatgtct ttagaattaa
    16501 agacaatacg aatcg
//
LOCUS       DAZGJW010000103        16452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZGJW010000103 DAZGJW010000000
VERSION     DAZGJW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.103"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..258
                     /locus_tag="ACHKIK_003388"
     CDS             <1..258
                     /locus_tag="ACHKIK_003388"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO9084500.1"
                     /translation="WGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAID
                     FDARTAIPFEGERHNALDDARHQAKYVSAIWQKLIPNQADF"
     gene            320..589
                     /locus_tag="ACHKIK_003389"
     CDS             320..589
                     /locus_tag="ACHKIK_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO9084501.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            558..1676
                     /locus_tag="ACHKIK_003390"
     CDS             558..1676
                     /locus_tag="ACHKIK_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO9084502.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            1843..2637
                     /gene="potC"
                     /locus_tag="ACHKIK_003391"
     CDS             1843..2637
                     /gene="potC"
                     /locus_tag="ACHKIK_003391"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO9084503.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2634..3680
                     /gene="potD"
                     /locus_tag="ACHKIK_003392"
     CDS             2634..3680
                     /gene="potD"
                     /locus_tag="ACHKIK_003392"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO9084504.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            3738..4199
                     /locus_tag="ACHKIK_003393"
     CDS             3738..4199
                     /locus_tag="ACHKIK_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO9084505.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSCP"
     gene            4196..4984
                     /locus_tag="ACHKIK_003394"
     CDS             4196..4984
                     /locus_tag="ACHKIK_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO9084506.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(5123..5944)
                     /gene="cobB"
                     /locus_tag="ACHKIK_003395"
     CDS             complement(5123..5944)
                     /gene="cobB"
                     /locus_tag="ACHKIK_003395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO9084507.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(5960..6871)
                     /gene="nagK"
                     /locus_tag="ACHKIK_003396"
     CDS             complement(5960..6871)
                     /gene="nagK"
                     /locus_tag="ACHKIK_003396"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO9084508.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(6900..8144)
                     /gene="lolE"
                     /locus_tag="ACHKIK_003397"
     CDS             complement(6900..8144)
                     /gene="lolE"
                     /locus_tag="ACHKIK_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO9084509.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(8144..8845)
                     /gene="lolD"
                     /locus_tag="ACHKIK_003398"
     CDS             complement(8144..8845)
                     /gene="lolD"
                     /locus_tag="ACHKIK_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO9084510.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(8838..10037)
                     /gene="lolC"
                     /locus_tag="ACHKIK_003399"
     CDS             complement(8838..10037)
                     /gene="lolC"
                     /locus_tag="ACHKIK_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO9084511.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            10299..11372
                     /locus_tag="ACHKIK_003400"
     CDS             10299..11372
                     /locus_tag="ACHKIK_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO9084512.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            11500..14946
                     /gene="mfd"
                     /locus_tag="ACHKIK_003401"
     CDS             11500..14946
                     /gene="mfd"
                     /locus_tag="ACHKIK_003401"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO9084513.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            15090..16052
                     /gene="ldtC"
                     /locus_tag="ACHKIK_003402"
     CDS             15090..16052
                     /gene="ldtC"
                     /locus_tag="ACHKIK_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO9084514.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(16135..16392)
                     /gene="bhsA"
                     /locus_tag="ACHKIK_003403"
                     /gene_synonym="ycfR"
     CDS             complement(16135..16392)
                     /gene="bhsA"
                     /locus_tag="ACHKIK_003403"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO9084515.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         4160 a         4233 c         4137 g         3922 t
ORIGIN      
        1 tggggaaatg gagccaactt cgacaacacg attttgcgcc gttcatacga acggcagggg
       61 atcccctgcc cgtggcgtta ctacaacgat cgcgatgtac gcacaatcgt tgagctgggg
      121 aaagccatag acttcgatgc cagaacggct attccattcg aaggtgagcg ccataatgca
      181 cttgatgacg ctcgtcacca ggcaaaatac gtttcagcta tctggcaaaa actgatcccg
      241 aatcaggctg atttttaatg ttcaaccgtc gccagttgtc gttgatattc tgcaactggc
      301 gcgttccgga gtgatagcca tgagcgaaca gtacctgata acgctcgacg agtggaaacc
      361 aaaacggttc agtctcccaa taacaaacac taccctggtg aaatacggaa aactaggata
      421 catcgttcca agaccacaaa aaattcgtgg gcgttggctg atagatcgcc gagcagtatt
      481 tgttgggcct ggtgaaacgg gaattgcgcc ggaaattcat actggcgatg atgatgcact
      541 gaaggagatt ttaactcatg tcaccgaggc cacgaaaaaa cagcactgac gtagccggtc
      601 tttacgaaaa gtttgatcgc agaactggca gagtttacta ccagtataaa aatcctgtga
      661 ctggaaaatt tcacggactc ggaacagaca aaggtaaggc agaaaaaatc gcttccacag
      721 ccaatcagcg aatagctgca gcagaagctg aatatttcat gcgcaaaatt gatgaaagtc
      781 cgtcagcaac aaaacgtcgg ggtatcagat taaaggcatg ggttgatcga tatctgaaaa
      841 tacaggacac gcgactgaaa aatggagata ttgcagctac aactcacaaa gaaaaaaccc
      901 gaatggctgc atacctggtt tcccgtctgg gaaaccaccc attgaaagaa ctggaagtaa
      961 gagactttgc attaatactg gatgagtggc tggataaaga catggtcagc acagcgagag
     1021 taaatcgtgg attatgggtt gatatttata aagaagcaca gcatgcaggg gaagttcctc
     1081 ctggatggaa tcctccggag gctacccgta aaccgatccc taaagtaacc agagccaggc
     1141 tcaccatgga agactggcaa aaaatttaca atgcaacgcc tgaaaaacac tttatccgta
     1201 acgcaatgct tcttgcgatt gttactggtc agcgccgtga tgacatttgc cacatgcgtt
     1261 tttcagatgt gtggaacgaa cacttgcata tcacccaggg aaaaaccgga atgcgtctgg
     1321 cgttaccgct tacactacgc tgtgatgcca ttgggataac gttaaaagaa gttattgatg
     1381 ggtgccgaga cagaatatta agtccatatc taatccatag tcggcaccag aaacaaccga
     1441 agccgatgag taaagacaac ctgagcgact actttgccaa agcacgggat ctggctggga
     1501 taattccacc agcaggaaaa actccgccaa catttcatga acaacgctct ctatcagaac
     1561 ggctgtaccg tgcacagggt attgatacaa aaacattact aggacataaa gtccaggcaa
     1621 ccaccgatcg ctataacgat actcgaggtc aggaatgggt taagttggtt atttgataaa
     1681 caccatatgg aatatccgtc accatctcag gaaaaaagtg ttgtaaaatg cgtggcctag
     1741 ttttggggaa acgttttgga gagattttgg agaaaggaaa aaattgaata aattcaaaat
     1801 cctgaaagtg tcccgcctta tgaataaaaa ggtggaactc gaatgatcgg tcgactgctt
     1861 cgcggcggtt ttatgaccgc tatctacgcg tacctgtata tcccaatcat tattttgatt
     1921 gtgaactcct ttaacagctc gcgctttggc atcaactggc agggttttac caccaaatgg
     1981 tatagcctgc tgatgaacaa cgacagcctg ttacaggcag cgcagcattc actaacaatg
     2041 gcggtgtttt cggcgacatt tgctacgctt atcggttcac tgacggcagt tgcgctgtac
     2101 cgttatcgct ttcgtggtaa gccgttcgtt agcggaatgc tgtttgtggt gatgatgtca
     2161 ccagatatcg tgatggcgat ttctctgctg gtgctgttta tgctgctggg tattcagctt
     2221 ggcttctggt cgctgctgtt ctcgcatatc accttctgcc tgccgtttgt ggtggtgacg
     2281 gtgtattcgc gcctgaaagg ttttgacgtg cggatgctgg aagcggcgaa agatctcggt
     2341 gccagtgaat ttaccattct gcgaaaaatc attctgccac tggcaatgcc tgcggtggcg
     2401 gcgggctggg tgttaagctt taccctgtcg atggacgatg tggtagtttc ttcgttcgtc
     2461 accggaccaa gttatgaaat tctgccgtta aaaatttatt cgatggtcaa agtcggcgta
     2521 tcgccggaag ttaacgcgct ggcaaccata ttactggtgc tgtcgctggt gatggttatt
     2581 gccagccagc ttattgctcg tgataaaacg aaaggtaaca caggggacgt taaatgaaaa
     2641 aatggtcacg ccacctgctc gcggcgggtg ctctggcact gggcatgagc gccgctcacg
     2701 ccgatgacaa caacacgctg tatttctaca actggaccga gtacgtgccg ccaggactgc
     2761 ttgaacagtt caccaaagaa accggtatta aggttatcta ttcgacttac gagtcgaacg
     2821 aaaccatgta cgccaagctg aaaacgtaca aagacggtgc ctatgacctg gtggttcctt
     2881 caacctatta cgtcgataaa atgcgtaaag aagggatgat ccagaagatc gacaagtcga
     2941 agttaacgaa cttcagcaat ctcgatccag acatgctcaa caagccgttt gacccgaata
     3001 acgattactc cattccgtat atctggggtg cgacggcgat tggtgttaac ggtgatgcgg
     3061 tggatccgaa atctgtcacc agctgggccg atctgtggaa gcctgagtac aaaggcagcc
     3121 tgctgttgac cgacgatgcc cgtgaagtgt tccagatggc gctgcgtaag ctgggctact
     3181 ccggtaacac caccgatccg aaagagattg aagctgcata taacgagctg aaaaaactga
     3241 tgccaaacgt cgcagcgttt aactccgata acccagctaa cccgtacatg gaaggcgaag
     3301 ttaacctcgg catgatctgg aacggttctg ctttcgttgc acggcaggcg ggtacgccaa
     3361 ttgacgtggt gtggccgaaa gaaggcggca ttttctggat ggacagcctg gcgatcccgg
     3421 caaatgccaa aaacaaagaa ggcgcgctga aattgatcaa cttcctgctg cgcccggatg
     3481 tggcaaaaca ggttgctgaa actatcggtt atccaacgcc aaaccttgcg gcgcgtaagc
     3541 tgttaagccc ggaagtggcg aacgataaaa cgctctaccc ggatgctgaa accattaaaa
     3601 atggtgaatg gcagaatgac gttggcgcag ccagcagcat ttatgaagag tattatcaga
     3661 agctgaaagc aggacgttaa ttcagcaagc gggcggtggc attaccgccc gttttttatt
     3721 tagcaaggat gtggctaatg agtcaggaca gtaaagtgtt atcccgcgct tttctcggta
     3781 tcggtctgat attgctcgtt atttctgcaa tcatctttta ttaccatttt acttttacca
     3841 aaagcgcggt tcatacagaa ggaatcattg ttgatgccgt ttggtacaac aatcactcaa
     3901 acgacgtcga tgataatggt tcctggtatc cagtggttgc ctttcgccct acgccagatt
     3961 acacgctaat tttcaattcg aatatcggta gtgattttta tgaagatagc gaaggtgaca
     4021 gagtcaatgt ctattatccg ccagggcatc cagaacaagc cgaaataaac aatccctggg
     4081 ttaatttttt taaatggggc tttgttggca tagcaggcat tattttcggt agtgtcggtc
     4141 tgataatcac gctcccctcc accaaaaaat ctcgcaggaa acggaagtcc tgcccatgaa
     4201 aaaattcatt atactgctca gtttactgat actcctgcct ctggtagcaa ccagtaaacc
     4261 attgattcca ataatgaaga cactttttac ggatgttacc ggtactgtac cggatgctga
     4321 agagatcgca cgtaaagccg agctttttcg ccaacaaacg ggaattgcac cgtttattgt
     4381 gattttaccc gacattaata atgaagccag tctcagacaa aatggtaaag cgatgctggc
     4441 gcatgcgtca tcttcattga gtaatgtaaa aggaagtgtt ctgttactat ttactacccg
     4501 cgaaccacgg ttaattatga tcaccaacgg ccaggttgaa agtggtctgg acgataaaca
     4561 tctcggcctt ctgatagaaa atcacacgct ggcttattta aatgcagatc tctggtatca
     4621 gggaatcaat aatgcattgg ctgttctaca agcacagata ttaaaacaac cgacgccgcc
     4681 actgacgtat tatccgcatc caggacaaca gcatgaaaat gctccccccg gcagcaccaa
     4741 cacattaggt tttatcgcct gggctgcgac gtttatctta ttttcctgga tcttttgcta
     4801 cacgactcgt tttatttatg cgttaaaatt tgcagtggcg atgactattg ccaatatggg
     4861 ttatcaggct ttatgtttgt atatcgataa tagctttgcg attaccagaa tatcgccact
     4921 ctgggcaggg ctaattggtg tctgtacgtt tattgccgca ttgttattga cgagtaaacg
     4981 ataaaagagg ccggaaaagt gcgcgtgtat tatgccggat ggggcgcgaa cgccttatcc
     5041 ggcccacaaa acccgcaaat tcaattaatt gcgtcccctt gtaggcctga taagcgtagc
     5101 gcatcaggca atgcttcctg ctttataatc ccttcagcaa cttttcaaca aactcaggca
     5161 ccacctggct tgccgggccg taatatttct cggcaaattc attaccgacc tgacttggtt
     5221 cgagattcag ttccacggtg tgcgcgccat gcagtttcgc ttcgtgaaca aacccagccg
     5281 ccggataaac atgcccggaa gtaccaatgg caatgaaaat atcagccatc gacaacgcca
     5341 tataaatttc atccatgccg agtggcattt cgccaaacca aacgacgtgc gggcgcaagg
     5401 gtgccgggaa ctggcaacaa tggcatttat cttctggcgt aacgtctccg gtccagtcga
     5461 gaacctgacc actttgcgaa caacgaactt tcagcaattc cccatgcata tgaatgacat
     5521 tggtattacc tgcgcgttca tgcaggttgt ctatattctg cgttaccagc aaaaagcgat
     5581 cgccgagggc gtcttgcagt ttagccagcg caagatgcgc ggcgttaggc tgaatttctg
     5641 gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg caccagttca ggatcgcgat
     5701 cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc ttcccacagg ccatctgcgg
     5761 cgcgaaaggt acgaatacct gattccgcag aaatccctgc ccctgtcagt acgagtactc
     5821 ttggtttttc cattgcttcc ggcaccactt tatctctgaa aaaaatacgc tgacgcaaac
     5881 gctcgcgcag gcggcgttta tttttacgaa aacgacttaa ccgatgaccc cgacgcgaca
     5941 gcatagcaac ctctgttgtt taatcggtta gatgtaggaa ggccgcacca cgcattcctc
     6001 ccgcatcacc gtggcgcgcg cgttcaatgc gcggaacacg agctacaggt aagagatgac
     6061 gaggcagcct gtccgccagt tgcgttgtga ttgccgggaa attcgataag ccaccaccaa
     6121 tgacgaccag gtcagggtca acaatggtca ggatatttcc cagacaaacc gctaataaat
     6181 ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc ttgatcataa agcgcaataa
     6241 tttcgggagc ctgcaacggt tgatgataat agtgttgata cagccacgca aaaccgcgac
     6301 cagacagata attttcaatg cagccatgct gaccacagcc gcagcggcgt aacgggaaat
     6361 ccaaccccat catggttaac gcatcaaccg gcagacgcat atggccaaac tcgccggtaa
     6421 tgtagctttt accggtaatc ggtttgccgt tgaaaatcag cccgccgcca acgccggtgc
     6481 cgagaatcaa ccccatcacc agtggatatt gagtaaattc atcatcccag gcttctgaaa
     6541 gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg atcaagacgt gcgctcaggt
     6601 cggcacgcag cggtttaccg ctggcagcgg ggacattggc ggcatacagc gtaccatctt
     6661 ctgtttccgg cattcccgga ataccgatgc cgacagaacc tttacagcca aaacgttggt
     6721 cagcttcagc taccagctca cacactgcat ctaaaaatgc gtcatagctg tcacgcggtg
     6781 tcggcacccg cttttcccac tgcaactgcc gaccgctatc aaacacgcca agcgcaattt
     6841 ttgttccacc aatatcaaac ccgtaataca ttgccgctcc ttgttttaat gtactgcctt
     6901 tactggccgc taaggactcg cgcagggtca atattactgg cgcgccgcgc cggataccaa
     6961 cttgccaaaa gactcagcaa caatgctgtg accagtacgt agaagacgtc cagccagtgc
     7021 aattccgatg gcaggaaatc aataaaatag atatcgctgg agaggaactg atgaccgatc
     7081 aacttttcaa tccactcaat aatcggggta agttgcagtg aaaccaccac accgataatc
     7141 accccacaca ggctgccgaa cagccctgcc agcaatccat accagacaaa gatggcgcga
     7201 attaaaccat ctttcgcccc cagcgttctt aatactgcga tatcgccact cttgtctttc
     7261 accgccatca ctaaggtgga gacgatgttg aaacaggcca cgccaatcac cagtaccatc
     7321 gccagataca taatggcgcg gatcatctgg atatcgcgat acatatagcc gtaagtacca
     7381 atccaacttt taatataaac atagctgttg gtcacttcac ccgcatcgcg taccagctta
     7441 ttggcgttga aaacatccgt cattttaagg gcaatacctg acacgctgga acccatatca
     7501 agatattgtt gggcatccgc cagcgggatc atggcaaaac tgtgatcgag ttgaccactc
     7561 aactgcaaaa taccggcaac gtgcaaacgc acacgttttg gctgcatcag tttatgctcg
     7621 ggattcgagt tggggatcat aatcgacacc caatccccct gcttcacttt cagcgcatcc
     7681 gccacgcctt tgccgatgat aatttgctgt tcgcccgctt taaaattgcg ccacgcatcg
     7741 ccctggacaa acgagggtaa tgcgctcaga cgctgttcct gttgcgggtt aacgcccttc
     7801 acctggattg cgcgaagatt cgcgccactt tccaccagcc cggtgaaatt gatatacggc
     7861 gcggcagcgg caatacccgg cactttttgc acgtgatcca gtgcttcctg ccagttagtc
     7921 cacggctgat ccaccgcctc gatttcacca tgcggcacca ccgccagaat acggttgttc
     7981 agttcgcgtt caaagccgtt catcgcgctt aagccgacga tcaataccgc cacgccgagg
     8041 gcaatgccaa tggtagaaat aacggagatc agcgacacca tgccgccgcg ccgccgtccg
     8101 cgactaaaac gcaggccaat taataacgat aaaggcatcg ccattactcc gcccccatca
     8161 ggctcagttc cgccgtcaga cgaccatcac gcatttctaa ctggcggctc atacgtttcg
     8221 ccagttgcag gtcgtgagta accaccagga aggcggtgcc ctgcaagcga ttcaattccc
     8281 caagcaactg gaagatgctg tcggcgttac gcgcatcgag gttaccggta ggttcatccg
     8341 ccagtaccag gcgcgggtta ttcaccagcg cacgggcaat cgccacacgc tggcgttcgc
     8401 cgccagaaag ttcagatggg cggtgattcg cacgatgatc cagccccacc gcttttaaca
     8461 tctcaagtgc acggctgttg atttcagcgg gctttttctt gccaatcagc agcggcatag
     8521 ccacgttttc cagggcggta aaatccggca gcaggtggtg aaactgataa ataaagccca
     8581 gcttctggtt gcgcagctct gctttcgccg ccgaagacag tttgctcatc ggttgaccgt
     8641 taaagatcac atcgccggag gttggcgtat ccagcccgcc caacaagtgc agcaaggtgc
     8701 ttttaccgga accagagcta ccgacgatcg ccatcatttc accttcgccg acgctgaaac
     8761 tgacattgtg cagcacatcg gtttgcacac tgccttcctg atagcgtttg cacaggttgt
     8821 cgcattgcaa caggatctta ttcataacgt aaagcctcag cgggttgagt ggcggcagcg
     8881 cgccatgaag ggtaaagcgt agacagcagc gcgatagcca tcgccaccag cgcaataaca
     8941 atgacctgta aaggttcgat agcgaccggc agcgccgcgc catcaagcag gacgccgatt
     9001 atcggcatca gattatttaa ctggctggca agcagtgcgc caagcgccgc accgaggatc
     9061 gcaccgataa tcccggcgct ggccccttgc accataaaga ccatcatgat ttgtcgcgga
     9121 gttaagcctt gcgtttgcag gatcgcgact tcgccctgct tctccattac catcagccct
     9181 agtgaggtaa taatgttaaa cgccgcaacg gcgacaatca ggctcagcag caagcccatc
     9241 atattttttt ccatgcgtac ggcctggaac agctcgcctt tacggtcacg ccagtcctgc
     9301 catttgctgc cttcaggcag tttttgctga cttaaagagt caactttcag cggctcatcc
     9361 agccacaaac gccagccggt aatattgcct gccggataac gcatcagacg cgaggcatcc
     9421 tcaatattca ccagcatttc atagccatcg acttcactgt tggcggcgaa agtgccaatc
     9481 acattgaaca ggcgctggct tggaatacgc cccatcggcg tgaactggct ggcagatggt
     9541 accatcacgc ggatttgatc accgcgatta acgcctagct gtgaggcaag ttgttcgccg
     9601 aggatgacat tatatttccc cggctcgagg tcagtttgtt tcacattgac cagatacggt
     9661 gtaagtggat ctttttgcgc cgggtcgata ccgagcatca ccccgaccgc cacgctgcgc
     9721 gcgctttgca gtaccacatc accggtagta ataggtgcga cgcgattaac gccgtccagt
     9781 ttgactgccg tttccgggag ttgctgcggg ttaagagagc catgctcaga agagagaatt
     9841 gcctgtggca tcaggccaag gatgttgttt tgcagctcgc gctcaaagcc gttcatcact
     9901 gacaatactg tgaccagcgc catcaccccg agggtaatgc cgatggtaga aagccaggag
     9961 acgaaacgac cgaagcgatc cgctgcacgc ccacgcatgt aacgcaggcc aataaataga
    10021 gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc aaagacacga gtatataagc
    10081 gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt cagggctaat ctgaaaagcc
    10141 cccgtaaatc aatcggctaa attgccatgt tttatcatca atcttgccgc ctgatcttaa
    10201 tctgaaaaat catccaatac agacagatcc ccattacacc tcgggtcata acccgcagga
    10261 tcaaccgcat gtaaattaaa aaagagcgtg ttgtcctgat gaaacaaaaa gagctatgga
    10321 ttaaccagat caaagggtta tgtatttgtc tggtggtgat ttatcactcg gtcattacct
    10381 tttatccgca tctgaccact tttcagcatc cgttatcgga agtcctgagc aaatgctgga
    10441 tctatttcaa tctttacctt gccccctttc gtatgccggt ttttttcttt atatccggct
    10501 atttgattcg ccgctatatc gacagcgtac cgtggggaaa ttgtctcgat aaacggatct
    10561 ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca gtggctggcg ttaagtgcgc
    10621 tgaaccagtg gctggcacct gagcgcgatt taagcaatgc ctccaatgcc gcttatgccg
    10681 attccaccgg tgagttcctg cacgggatga tcaccgccag caccagcttg tggtatctgt
    10741 atgcgttaat tgtctatttc gtggtatgta aaatttttag ccgtctggcg ctgccactat
    10801 tcgccttgtt tgtactgctg agtgtggcgg ttaatttcgt tcccacgccg tggtggggaa
    10861 tgaacagtgt gatccgcaat ttgctttatt acagccttgg cgcatggttt ggcgcaacaa
    10921 taatgacctg tgttaaagag gtgccgttgc gccgccatct gctgatggct tctttgctta
    10981 ccgttctggc ggtcggtgcc tggttgttta ctatctcgct gctgttgtcg ctggtatcga
    11041 ttgtggtgat catgaagctg ttttatcaat acgagcaacg attcggtatg cgctccacca
    11101 gcctgctgaa tgtgattggc tccaacacca ttgctatcta caccacccat cgcattctgg
    11161 ttgaaatatt cagcttaact ctgcttgcgc aaatgaacgc agcacgctgg tcgccgcaag
    11221 tcgaactgac actcctgctg gtttatccct ttgtcagttt gttcatctgt actgttgcgg
    11281 gcttgctggt aagaaaactt tcacagcgtg cattcagcga tctgttgttc tccccgcctt
    11341 cactgcccgc tgccgtcagt tactctcgtt aaaccatcgc cccagccggg gcgattcaat
    11401 ttgctaaacc atgtcgatta cggataatga acagcattgc ttatcagaga tatgccccca
    11461 tatgttgagg catatcctaa cgagaatctg acaaccgtta tgcctgaaca atatcgttat
    11521 acgctgcccg tcaaagcggg tgagcagcgt ctgctgggcg agttaaccgg cgcagcctgt
    11581 gcaacgctgg tagcggaaat tgccgaacgt cacgccggtc cggtggtact cattgcacca
    11641 gatatgcaaa atgctctgcg tttgcatgat gaaatcagcc agttcaccga tcagatggtg
    11701 atgaatctgg cggactggga aactcttccc tacgacagtt tttcgcctca tcaggacatt
    11761 atctcctcgc gcctttccac cctttaccag ctaccgacga tgcagcgtgg cgtactgatt
    11821 gttccggtga atacgcttat gcagcgcgtt tgcccacaca gttttctcca cggtcatgcg
    11881 ctggtgatga aaaaaggtca gcgcctgtca cgagatgcat tacgaaccca actggacagc
    11941 gccggttatc gccatgttga ccaggtgatg gagcacggcg aatacgccac gcgcggcgcg
    12001 ttgctggatc tcttcccgat ggggagtgag ctgccttatc gtcttgattt ctttgatgat
    12061 gaaatcgaca gcctgcgggt gtttgacgtc gacagccagc gcacgctgga ggaagtagaa
    12121 gcgatcaatc tgctgcccgc gcacgaattt ccgaccgata aagcggcaat tgaactgttc
    12181 cgcagccagt ggcgcgatac cttcgaagtg aagcgcgatc cggaacatat ttaccagcaa
    12241 gtgagtaaag gcacattacc tgccgggatc gagtactggc agccattgtt cttcagcgaa
    12301 ccactgccgc cgctgttcag ttatttccct gccaatacct tgctcgtgaa tactggcgat
    12361 ctggaaacca gtgccgaacg tttccaggct gacacgctgg cgcgttttga gaatcgcggc
    12421 gtcgatccga tgcgcccgct gttgccacca caatcgctct ggctgcgggt ggacgagctc
    12481 ttctcagagc tgaaaaactg gccccgggtg cagctaaaaa ctgaacattt accgacaaaa
    12541 gccgcgaatg ccaatttagg tttccagaaa ctgccagacc tggccgttca ggcacaacaa
    12601 aaagcgccgc tggatgcgct gcgtaagttc ctcgagactt tcgacggtcc ggtggtgttc
    12661 tcggtagaaa gtgaaggtcg ccgtgaagcg ctgggtgaac tgctcgcacg aattaaaatt
    12721 gctccgcaac gcattatgcg tcttgatgaa gccagcgacc gtgggcgtta tctgatgatt
    12781 ggcgctgccg aacatggttt tgtcgataag gtgcgtaatc tggcgctgat ctgcgaaagc
    12841 gatctgctcg gtgaacgtgt tgcccgccgt cgtcaggatt ctcgccgcac catcaacccc
    12901 gatacactga tccgtaacct tgcggagctg catattggtc agccggtggt ccatctggag
    12961 cacggtgtcg ggcgctatgc cggaatgacc acgctcgaag cgggcggcat tactggcgag
    13021 tatttgatgc tcacctatgc caacgacgcc aaactgtatg ttccggtgtc gtcactgcat
    13081 ctgattagcc gttacgcagg tggcgcggaa gaaaacgccc cgctgcataa acttggcggc
    13141 gatgcgtggt cacgcgcgcg gcagaaagcg gcggaaaaag tgcgtgatgt ggcggcggaa
    13201 ttgctggata tctacgcgca acgcgccgcc aaagagggct tcgcgtttaa acacgatcgt
    13261 gagcagtatc agttgttctg cgacagcttc ccgtttgaaa ccacgccgga tcaggcgcag
    13321 gccattaatg cggtacttag cgacatgtgt cagccgctgg caatggatcg tctggtgtgc
    13381 ggcgatgttg gctttggtaa aacagaagtg gcgatgcgcg ccgctttcct ggcagtagat
    13441 aaccacaagc aggtggcggt gttggtgcct accacccttc tcgcgcagca gcattacgac
    13501 aacttccgcg accgtttcgc caactggccg gtacgtatcg aaatgatctc ccgtttccgc
    13561 agcgccaaag agcagacgca aatccttgcg gaagtggcgg aagggaaaat cgatattctg
    13621 atcggtacgc acaaactgct gcaaagtgac gtcaagttta aagatttagg cctgctgatt
    13681 gtcgatgaag aacaccgctt cggggtgcgt cataaagagc gtattaaagc gatgcgcgcg
    13741 aacgtggata ttctgacgct tactgcaacg ccgatcccac gtacgctgaa tatggcaatg
    13801 agcggaatgc gtgacctgtc gattatcgcc acgccgcccg cccgtcgtct ggcagttaaa
    13861 acctttgtcc gtgagtatga cagcctggtg gtccgggagg cgatcctgcg tgaaattttg
    13921 cgcggaggac aggtttatta tctctacaat gatgtggaaa acattcagaa agccgccgaa
    13981 cggctggcag aactggtgcc agaagcgcgg atcgccatcg gtcacgggca gatgcgcgag
    14041 cgcgaactgg aacgggtgat gaatgatttc catcatcaac gtttcaacgt gctggtttgt
    14101 acaaccatta tcgaaaccgg gatcgacatc ccgacagcca acaccattat cattgaacgc
    14161 gcggatcact tcggtctggc gcagctgcac cagttacgcg gtcgcgtcgg acgttcacat
    14221 catcaggcat atgcatggtt gctgacgccg catccaaaag cgatgactac cgatgcacaa
    14281 aaacgtcttg aagcgattgc ctcgctggaa gatctcgggg caggttttgc gctggcaacg
    14341 cacgatctgg agattcgcgg cgcgggtgaa ctgcttggcg aagaacaaag cggctcaatg
    14401 gaaaccatcg gtttctcgct gtatatggag ttgctggaaa acgccgtcga tgcactgaaa
    14461 gccggacgcg agccgtcgct ggaagatctc accagccagc aaacagaagt cgagctgcgg
    14521 atgccgtcgc tattgccaga tgatttcatt cctgacgtga atacgcgttt gtcgttctat
    14581 aaacgtattg ccagcgccaa aacggaaaac gaactggaag agatcaaagt cgagcttatc
    14641 gatcgcttcg gcctgctgcc ggatccggcg cgtaccctgc tggatgttgc ccgtctgcgc
    14701 cagcaagcgc agaaactggg gatcaggaag ctggaaggta atgagaaagg cggggtgatc
    14761 gaatttgccg agaagaatca cgttaatccg gcctggttga ttggtttgct gcaaaaacag
    14821 ccgcagcatt accgccttga tggtccgacg cgcctgaaat ttattcagga tttgagtgag
    14881 cggaaaacgc gtatcgaatg ggtacgccag tttatgcgtg aactggaaga gaacgcgatc
    14941 gcttaatggt aaatcggggg cgtttctgcg cccccatctg caacatttac aaattctttg
    15001 cacttccctg cactatccga cactgtcacc atccataatt caggcttatc tgtttattac
    15061 aataacctta tatttattat ggatttttgg tgatgatcaa aacgcgtttt tctcgctggc
    15121 taacgttttt tacgttcgcc gctgccgtgg cgctggcgct accggcaaaa gccaacacct
    15181 ggccgctgcc gccagcgggc agtcgtctgg ttggcgaaaa caaatttcat gtggtggaaa
    15241 atgacggtgg ttctctggaa gccatcgcca aaaaatataa cgtcggcttt ctcgctctgt
    15301 tacaggctaa ccctggcgtt gatccttacg taccgcgcgc gggtagcgtg ttaacgatcc
    15361 cgttgcaaac cctacttcca gatgcgccgc gcgaaggcat tgtgatcaat attgcggagc
    15421 tgcgtctcta ttactacccg ccgggtaaaa attcggtaac cgtgtatcca ataggtattg
    15481 gtcagttagg tggtgacacg ctgacaccga caatggtgac caccgtttca gacaaacgtg
    15541 caaacccaac ctggacgcca acggcaaaca tccgcgcccg ttataaagca cagggaattg
    15601 agttgcctgc ggtagtgccg gctggaccgg ataacccaat gggccatcat gcgattcgtc
    15661 tggcggccta tggcggcgtt tatttgcttc acggtacgaa cgccgatttc ggcattggca
    15721 tgcgggtaag ttctggctgt attcgtctgc gggatgacga tatcaaaaca ctctttagcc
    15781 aggtcacccc aggcaccaaa gtgaatatca tcaacactcc gataaaagtc tctgccgaac
    15841 caaacggtgc gcgtctggtt gaagtacatc agccgctgtc agagaagatt gatgacgatc
    15901 cgcagctgct gccaattacg ctgaatagcg caatgcaatc atttaaagat gcagcacaaa
    15961 ctgacgctga agtgatgcaa catgtgatgg atgtccgttc cgggatgccg gtggatgtcc
    16021 gccgtcatca agtgagccca caaactctgt aaatcctgga taaaaaaaat ccccgcgaca
    16081 cggcatgtcg cggggaaaaa atcagcagta gcaggcatta atgagggtta atgcttattt
    16141 ataaattact gctgttccat ggagggtatt cggaccggtt acagaagtaa tacggaaaga
    16201 ttttgcgccc atctcatccg ctttttgcgc cagctgctct tccagcgatc ccagatttgt
    16261 ccccgcgtta gcactgattg taccgacttt ttgttggcct tctggcgttg actgaacttc
    16321 gacagccgca aagctggcaa atgacatgga gcttaaaatc gccgcagcga tgagggtttt
    16381 tacgtttttc ataatagtgg ccttatgcag atgaatgacg tcgcaagcaa ttacttaacg
    16441 atcgataact aa
//
LOCUS       DAZGJW010000104        16115 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZGJW010000104 DAZGJW010000000
VERSION     DAZGJW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.104"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            212..1111
                     /gene="yegS"
                     /locus_tag="ACHKIK_003404"
     CDS             212..1111
                     /gene="yegS"
                     /locus_tag="ACHKIK_003404"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO9084516.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1193..1966)
                     /locus_tag="ACHKIK_003405"
     CDS             complement(1193..1966)
                     /locus_tag="ACHKIK_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO9084517.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2072..3112)
                     /gene="gatD"
                     /locus_tag="ACHKIK_003406"
                     /pseudo
     CDS             complement(2072..3112)
                     /gene="gatD"
                     /locus_tag="ACHKIK_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
     gene            complement(3160..4515)
                     /locus_tag="ACHKIK_003407"
     CDS             complement(3160..4515)
                     /locus_tag="ACHKIK_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO9084518.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(4519..4803)
                     /gene="gatB"
                     /locus_tag="ACHKIK_003408"
     CDS             complement(4519..4803)
                     /gene="gatB"
                     /locus_tag="ACHKIK_003408"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO9084519.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(4834..5286)
                     /gene="gatA"
                     /locus_tag="ACHKIK_003409"
     CDS             complement(4834..5286)
                     /gene="gatA"
                     /locus_tag="ACHKIK_003409"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO9084520.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVYDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5296..6558)
                     /gene="gatZ"
                     /locus_tag="ACHKIK_003410"
     CDS             complement(5296..6558)
                     /gene="gatZ"
                     /locus_tag="ACHKIK_003410"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO9084521.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIESTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6587..7441)
                     /gene="gatY"
                     /locus_tag="ACHKIK_003411"
     CDS             complement(6587..7441)
                     /gene="gatY"
                     /locus_tag="ACHKIK_003411"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO9084522.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7751..8803)
                     /gene="fbaB"
                     /locus_tag="ACHKIK_003412"
     CDS             complement(7751..8803)
                     /gene="fbaB"
                     /locus_tag="ACHKIK_003412"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO9084523.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9060..10337
                     /locus_tag="ACHKIK_003413"
     CDS             9060..10337
                     /locus_tag="ACHKIK_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO9084524.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10334..11338
                     /locus_tag="ACHKIK_003414"
     CDS             10334..11338
                     /locus_tag="ACHKIK_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO9084525.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            11335..12300
                     /locus_tag="ACHKIK_003415"
     CDS             11335..12300
                     /locus_tag="ACHKIK_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO9084526.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12274..13020)
                     /locus_tag="ACHKIK_003416"
     CDS             complement(12274..13020)
                     /locus_tag="ACHKIK_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO9084527.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13072..13890)
                     /locus_tag="ACHKIK_003417"
     CDS             complement(13072..13890)
                     /locus_tag="ACHKIK_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO9084528.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            complement(13955..14755)
                     /gene="thiD"
                     /locus_tag="ACHKIK_003418"
     CDS             complement(13955..14755)
                     /gene="thiD"
                     /locus_tag="ACHKIK_003418"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO9084529.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14752..15540)
                     /gene="thiM"
                     /locus_tag="ACHKIK_003419"
     CDS             complement(14752..15540)
                     /gene="thiM"
                     /locus_tag="ACHKIK_003419"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO9084530.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15575..15671)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15763..16035)
                     /gene="rcnR"
                     /locus_tag="ACHKIK_003420"
     CDS             complement(15763..16035)
                     /gene="rcnR"
                     /locus_tag="ACHKIK_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO9084531.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
BASE COUNT         3944 a         4189 c         3929 g         4053 t
ORIGIN      
        1 ttggttgaat attaggcata tgctcttttt tgaaatttat cggtggcagc cgttagtatt
       61 cgctgtcccc attgcaagct cctggtggta accactgaat cctccatact tgaactgact
      121 ttttatcctc cgactttcat cctgttctga ctccaccttt tgttttctgc tctacactat
      181 ctacagacca atcataaagg cacatacgat catggcagaa tttcccgcca gcttactgat
      241 tcttaatggc aaaagtactg acaatctacc cttgcgcgaa gcaattatgc tgttgcgtga
      301 ggaaggaatg acgatccatg tgcgggtcac ctgggagaaa ggcgatgccg cacgatatgt
      361 agaggaggcc cggaagttgg gcgtcgcaac ggtgattgcc ggtggtggcg atggcaccat
      421 taatgaagtt tctacggcgt tgattcagtg tgagggggat gacatacccg cgctgggaat
      481 tttgccatta ggaaccgcca atgattttgc caccagtgta gggattcctg aggcactgga
      541 taaggcgctg aaactggcaa ttgccggtaa cgccattgcg atagatatgg cgcaggtcaa
      601 caaacaaacc tgttttatta atatggcgac aggcggattt gggacgcgta ttaccacaga
      661 aacgccggaa aaattaaaag ccgcgctggg tggcgtctct tacatcattc atggcttaat
      721 gcgcatggat actctgcaac cggaccgttg tgaaatccgc ggtgaaaact ttcactggca
      781 aggtgacgcc ctggtcattg gtattggtaa cgggcgtcag gccggtggcg gtcagcaatt
      841 gtgtccgaac gcgttaatta acgatggctt gctgcaactg cgcattttta ccggcgatga
      901 aattcttccg gctctcgtat caaccttaaa atctgacgaa gataacccga atattatcga
      961 aggcgcttcg tcgtggtttg atatacaagc cccacacgaa atcactttta atcttgatgg
     1021 cgaaccgttg agcggacaaa actttcatat tgaaatactt ccggcggcgt tgcgttgtcg
     1081 attaccacca gattgtccat tattgcgtta atcaaactat tctctcttga accagggaga
     1141 atccgaaatg ccggatgcgg tgtaaatacc ttatccggca tacagaacaa tactacaccg
     1201 taattaattt tacccgtgtc ttctgcaatg ccagtttatc cacctctgat acagcatcat
     1261 cggtaataat aatgtcaatt ttttcgattg gcagtacctg attaaaaccg cgacggttaa
     1321 atttcgatga atcgagaacg gcaatcactt tattagcggc ggtgaccatc gcaccgctaa
     1381 tcgaataacc ttcattaaag gtcgtaatac cattaaccgc atcaatacca tcagcaccga
     1441 caaacattaa atcggcatta atatcctgca gggaacgttc agcaatagat ccatgcatag
     1501 atcgcgtttt atggcggacc gtgccaccgc agacgaccag agtaatgtct ttattttcgg
     1561 aaagggcaaa cgcagccggg agactgttgg taatcaccgt gatatttttg gcggtcatta
     1621 atccttcggc gataagcatt gtggtacttc cgctgtcgag aataacggtc atcccttcat
     1681 ggatcatttt gactgccgcc tgcgctattc tgtttttcgg cgcgctggcg agctgaaatc
     1741 gctctttaaa cccgacttcc tgggtctcgg tttcactatt accggacatt attttcgccg
     1801 caccgccatg aaagcgcgta accacgcctt tttgttcgag aaagcgcaaa tcggcacgga
     1861 ttgtcgcttc cgaggcagca aatactcccg ccagatcctg aacaagcacg gttccctgtt
     1921 cattcactaa ttggatgatc ttattccttc gctcgaatga attcatcgtc atttacccat
     1981 attcaattgt ggctagtgta aacgaagggg gaccattggt gaatgatctg ttttgcacaa
     2041 taccgggcca gcatcacgct ggcccgcggt ttcagggaat gagcaacact ttgcccggca
     2101 tagcattacg agcgatgtca cgcaccgcct gggcgaagct ttcaaagctt ccacggtgag
     2161 cgattaatgg ctccaggctt aacttacgtt ctgtcagcaa ccggctcgcc gtttcccact
     2221 cctgccccgg ccaagggctg gagtagttca tccaactgcc gataaccgtc agctctttac
     2281 gcaatatttt gccaaacgtt gccgatgtta aatgcagatc ctgatgcaac gtgcccacca
     2341 gcgccagttg ggcatgagga ccggcaatct ctaccgccag ttcgacagtt tgcggtacgc
     2401 cagccgtctc gaggataagc tgattaaagc gcagttagcg taaaacgctc tgcatttgcg
     2461 gcgcgctcat ttcgctactg ttaaatgttt gcatcgcacc gaaagatttt gccagtgcca
     2521 gtttttctga actaatgtcg atcgccgtca cactctttgc tcccagcgcg acagcgcact
     2581 gaatggccag caggccaatg gttccggcac caataataat aacgttttta ttctcacaac
     2641 cttgcgctaa atgaaaagca tgcaggccaa cggtaatcgg ttcaataaaa gccccatcct
     2701 caataggcat atcagtaggt agagcaaaga catttttccg cttaacgaca atatattcgg
     2761 caaatccacc atcacgccgt gagccaataa aatcatattt tgcgcactgg gaataaaacc
     2821 ctttcaaaca ctctggacaa gtaaaacagg gtaataacgg cacacaggca accgcatcgc
     2881 caggatgtaa atcatcaaca ccggatccca ccgcatcaat atagccgcta aattcatggc
     2941 ctaacgttat tggataataa tgtgcaccat ttttaaatat cctgggtaaa tcggaaccac
     3001 ataagcccga gctggcaatt tttacccgca cctcatcctg atgtttaatt tcaggaatga
     3061 cgctttctgc aacgcgcacg ataccatcag tatcattcac cactgatttc ataaaaactc
     3121 ctgattgtta agggggataa cctccccctc aggtaaaaat tattctgcga gaacgacttt
     3181 ctcttgttta ataaagccac gcgctctacg ccaggtcatg aaaataccgg taagataaat
     3241 tgcgccgata ataatgaatc cgggaatatt ttgcggggag aaaacctgaa tcagtaacca
     3301 ggtaatggga gaaccgccct gatccattga agccaccata cccccggctt ttaacgcccc
     3361 agcattagcc gccagttggg tgtgcaaacc aatagtttgc gtcgcgatcc acagagtgat
     3421 gctcatgatg atgacacccg agattaaggt gcggaacaga tttccacgat gcacggcgac
     3481 tgccatcgcc acaaagaagc cgatggtggc aagatcgcca aacggcagca cctgattacc
     3541 cggaacacaa acagcaatta aaatggtgag cgggataaaa atcaggcttg ccgataccac
     3601 cgccgtatgc cccagcagta atgcgggatc caggccaatc aggaactcct gaccgccgaa
     3661 cttcgcctgt aaacgactac gggcctgctt agcgatgggc gttaaaccat ccatgatggg
     3721 tttaatcacc cgtggcatta gcagcatcac tgccgccgtt tttaccgcca gctgcaatac
     3781 acctttgaca tcgtaacccg cgaggatgcc gataatcagc cccatcacaa aacccacggt
     3841 gacaggctcg ccaaatggac caaatttgcg ctgaatatcg tcggcgctaa atttaattcg
     3901 gttaacgcct gggattttct cgatgatagc atcgaccagc accgcaatcg gccccatata
     3961 cgccgacgta ccgtgcggaa tagcaatacc ttccagctca aagaaatttc gggtatcgcg
     4021 ggcaaaccag tcgccgagct tataaacaaa cgccgcgtga attaccacac ctgccatccc
     4081 tatcatccat gaaccggttg ccaggtgcag caacgcgccg gtgaaggtca tatgccagat
     4141 attccagata tcaacattta ccacccgcgt catacgggtc agtaacatcg ccacgttaac
     4201 caatatcgca atcggaatcg ccaccagcgc aatttgcgac gcccaggtca ttggtgaaga
     4261 gcccggccag ccgacatcga ccacatgcag attcaggtcg aaattttccg ccatcgcttt
     4321 cgccgccgga ccaatggaat ccagcattaa gccaatcaca aggccaatgc caacaaagcc
     4381 aatcccgata tgcagacccg ctttaaagca atcgcctgcc ttcatgccta atattttaga
     4441 aaaaataatg atgacaatcg gaagcatcac cgtagggccg aggtcgagaa tataacgcat
     4501 gacttctgaa aacataggtc acccctgtaa gatagtcaga attttatttt gtaatgcttc
     4561 gataccgaca ccagaaataa aaggcatgcc gtgaactaac ggaatatcgc caaaactacg
     4621 atccactttg gcagtggtgc atatcaaatg cacaccatcc atataggttt ctatttcatt
     4681 aacccgacac tggattaatt caacaggaat attatgattc tgacacaact ctttaatttc
     4741 ttccgccgcc atcgtagagg tcgcaaccgc gcctccgcaa gcgacaataa tcttgcgttt
     4801 cataaatagt cctttttatt gttacagagg gatttattcg tctgaatcta aaacatactt
     4861 tgtgaagtat tcctttaact gagtttcagg aagagtaatt agtgtctcga cgatatcggg
     4921 ctgttgtaac ttgccaaata aacagcgtaa aagtttcaat tgctgctgcg gattttccac
     4981 aatcaacgca ataaccaacg acacctccac gtcgttatca tcatccgctt gctgaaaatg
     5041 aactttattt gttggcctta acagataaat ggctgacgac ttagcatgaa tcgcctcaca
     5101 atgcggtata gcaatggcgt gctgctcaag cattatcccg gtagggaatt ctgcttctct
     5161 ggcaattaat gcctgtggcc aggtatcata aaccacacct ttcgcgagca tctcattacc
     5221 gatatgggtt aaaacttcgc tacgatcgac aaaagaaatt ccgctacgaa caaacaggtt
     5281 agtcatatac cgtccttatt ccgcacagcc gtagcgatag gcgcgcaaaa catcataaat
     5341 tttatccata atcagctgat gcggtattgc tgataattcc ccggactgaa tgcgttcaaa
     5401 ttgtttggga agatactgac taatcatgcc cagtgggatg tccacgcctt caagattcac
     5461 catcatcgtt tcgacgctat ttttaatccg actatgcggc cagtaataac gaatacgatc
     5521 cgacaggctg taacgaatat ccagtaatga atcgttaaaa cccgtacgat aatatttttt
     5581 ccagtattgc ggttcgtcga gcatcacttc ttcaattacc gccaggcaac cgctgcgatt
     5641 ttcaggggcg ataagttcct gctcaatttg tgccagtgca aatatcgcct cgcgtaaagc
     5701 aaaggttaat gcgggaccga ctttcaatat tgcaaagtga tcgcggacta attcccaata
     5761 agccgtccgg gtctggtaat cggtagaatg tgcttcataa accattcggg tgctttctat
     5821 ccattgcgcc agcggctgcg cttcctgcgg ctgataatgg ataatattgc tgtgatcaaa
     5881 ttccacgccc ggctgcacca cgatggcaat cacgcgtgtt aacgcctctg tcagcccacg
     5941 ggcaataaag gccttttgat gcgtacgtaa agtattggcg gcatcttcaa catgggtgat
     6001 gtgtactgac tgaatggcgc tggcctcacc gcccggaacc ggaacttcgg tgccaatgac
     6061 atagctcagt tgctcacgct ggcaatctgt cgccacactt tccgcagcaa agcaaagcac
     6121 agcagctcgt tccgcaaccg tttctggtgc taacggtatg ggatcccccg cgcaggacat
     6181 tgacgcatca agatgaattt tactgaaacc ggcacgaaca tattccttta ccagctcgac
     6241 ggatttttcc atcgccgcat ccgcattttc ttgctgccag cagtttggcc ccagatgatc
     6301 gccgccgaga ataatgcgtt cgcgtgcaaa cccaacttta tcggcaatcg taaaaacaaa
     6361 ttcgcgaaag tctgccggtg tcattccggt ataaccgcca aattgattga cctggtttga
     6421 cgttgcttca atcagcactt tgcgcgtgct gttgcgatca aatgccagcg ccgcttcgat
     6481 aaccaacgga tgggcagaac agactgaaca tatgccgata tgttcaccag ctttatgccg
     6541 ggcaattaac gttttcatgt tttttcctta ttaaatggca ggtgcgttat gccctgccct
     6601 cgcagccaca atcggcaatc actttgctca ccacatcgcg cattgcggat ttagccgact
     6661 gcaaataatc ccggggatcg gtcgcttcag ggtgctcggt caggtaattt tttaacgcct
     6721 gcgagaaggc atttttcagc tccgttgcaa cgttgatttt gcatatcccc agtttgatgg
     6781 tttgctgaat atccttagtc gataaccctg acgcgccatg cagcaccagc ggtaagttca
     6841 cccactggcg aatgttctcc agtcgagaaa aatcaagcgc cggtgcgctg gcatacatcc
     6901 catgagccgt gccgatggcg accgccaggg aatcaattcc ggttgcctcg gcaaattcac
     6961 gcgcctgagc ggggttggta taaaaagcat cggcttcatt gacttgcaca tcatcttcct
     7021 ggccgccaag ttgccccagc tccgcttcga cgctgacatc aaagcgatgg caaaaatcca
     7081 ccacctcttt gacccgtgaa atattttgcg caaaaggcaa atgcgaggcg tcaatcatga
     7141 ctgagcgcac gccagaacga accttctgag cgatatcgtc aaatttcgtg tgatggtcga
     7201 gatgaattgc cagtggatgg tgatattgct tcgccatcgc gctgaccagc gccaacagat
     7261 tttctgtacc agcatgagta aatgtgccag gcgttccggc gatgatgacc ggcgcatgca
     7321 ggttggcagc ggtttccacc accacttgca tcgtttcgag attgtgaata ttgaatgccg
     7381 gaaccgcata accgccgcgc tgtgcgttgt tcagcatctg ctttgtcgat accacgtaca
     7441 ttttcatatc ctgtcgtttg ttttcgattt caaaatataa tgaaatgatt tgtttttaaa
     7501 tgtcgagata acgatcacaa aagcgacaat gtgaaaattg ttcgaggagt aaaaggcaaa
     7561 aaaaaacggc ctcccgatag ggaagccgta gcaaagtgcg cgtgttttta tgccggatgc
     7621 ggtgtaaaca tacgtggcag tgctatgtag gcatgataag acgcttccgc gtcgcatcag
     7681 gcataggttg ccggatgcag tgtaaacgcc ttatccgtcc tacggggtgg tgctgttcgt
     7741 atgaggcgta tcaggcaata gtaattttgc tatcgagata aacgtcctgc acggcattaa
     7801 tcagtttcac gccgtcagcc atcgatttct tgaacgcctt acgtccaaga atcagcccca
     7861 ttccgccagc gcgtttgttg ataaccgcag tacgcactgc atcgctgagg tcagtttcac
     7921 cgcccgcagc accgccggag tttatcaacc cagcccgacc catatagcag ttagctaact
     7981 gataacgcac cagatcaatc gggttttcgc tggtcaattt gctgtaaaca cgatcgtcgg
     8041 tgtaaccgta attaattgct ttatagccgc cgttattttc cgccattttt tgtttgacga
     8101 tatctgcacc gatggttgcc gccagatggt ttgcctgacc ggtcaggtcg gcggaaacat
     8161 ggtaatcaac gccatctttc ttaaaggcgg agttacgcaa ataggcccac agcactgtca
     8221 ccatacccag ctcgtgcgca cgttcaaaag ccgcagaaat ttcttcaatc tggcgacgtg
     8281 actcttccga gccaaaatag atagtcgcac caaccgcaac cgcgcccatg ttgaacgcct
     8341 gctccacgct ggcatacagc gtttgatcgt aggtattcgg gtaacttagc gtctcgttgt
     8401 gattaagttt gacgaggaat ggaatgcgat gcgcataacg ccgcgatacc gacgccagca
     8461 cgccgtaagt tgacgccaca cagttacagc ccgcttcgat cgccagttca acaatgtttt
     8521 tcgggtcaaa gtagagcggg ttagcagcaa atgaagctcc ggcagagtgc tcaacgccct
     8581 ggtcaaccgg cagaatagaa agatatcctg tgccagccag acgcccggtg ttgtacaacg
     8641 tctgcatatt acgtaacacc gctggcgggc gattattgtc aatcattacg cggtctacgt
     8701 agtcatgtcc ggggagataa agctggtcag aaggaattgt catacaacgg tgctgtaaaa
     8761 ggttgtcggc gtctttgcca agcaactgcg caatatctgt cattactatg ctcccgtaaa
     8821 ttccgattgg atatcggcta tggattgtcc tggcccgcct tttgcgggca atcataatcc
     8881 tggtcgttac gggcgagttt ttccatcttt tggatgtttt ttcagcgttt tctgttggct
     8941 cgattcatca gaaaaaatgt tagcgcggtc aagttttcac cgcaaaggta tttaaaaggt
     9001 attattaagt ggtattgtca tcgcgtacct tacattacct gtcatgaagg aattaaaaga
     9061 tgaaaacaac agcaaagctg tcgttcatga tgtttgttga atggtttatc tggggcgcgt
     9121 ggtttgtgcc attgtggttg tggttaagta aaagcggttt tagtgccgga gaaattggct
     9181 ggtcgtatgc ctgcacagcc attgcggcga tcctgtcgcc aattctggtt ggctccatca
     9241 ctgaccgctt tttctcggcg caaaaagtgc tggcggtatt gatgttcgca ggcgcgctgc
     9301 tgatgtattt cgctgcgcaa cagaccactt ttgccgggtt cttcccgtta ctgctggcct
     9361 actcgctaac ctatatgccg accattgcgc tgactaacag tatcgctttt gccaacgtgc
     9421 cggatgtgga gcgtgatttc ccgcgcattc gtgtgatggg cactatcggc tggattgcct
     9481 ctggtctggc atgtggtttc ttgccgcaaa tgctggggta tgccgatatc tcaccgacta
     9541 acatcccgct gctgattacc gccggaagtt ctgctctgct cggtgtgttt gcgtttttcc
     9601 tgcccgacac gccgccaaaa agcaccggca aaatggacat taaagtcatg ctcggcctgg
     9661 atgcgctgat cctgctgcgc gataaaaact tcctcgtctt tttcttctgt tcattcctgt
     9721 ttgcgatgcc actggcgttc tattacatct ttgccaacgg ttatctgacc gaagttggca
     9781 tgaaaaacgc caccggctgg atgacgctcg gccagttctc tgaaatcttc tttatgctgg
     9841 cattgccgtt tttcactaaa cgctttggta tcaaaaaggt attattgctt ggtctggtca
     9901 ccgctgcgat ccgctatggc ttctttattt acggtagtgc ggatgaatat ttcacctacg
     9961 cgttactgtt cctcggtatt ttgcttcacg gcgtaagtta cgatttttac tacgttaccg
    10021 cttacatcta tgtcgataaa aaagcccccg tgcatatgcg taccgccgcg caggggctga
    10081 tcacgctctg ctgccagggc ttcggcagtt tgctcggcta tcgtcttggc ggtgtgatga
    10141 tggaaaagat gttcgcttat ccggaaccgg taaacgggct gactttcaac tggtccggga
    10201 tgtggacttt cggcgcggtg atgattgcca ttatcgccgt gctgttcatg atttttttcc
    10261 gcgaatccga caacgaaatt acggctatca aggtcgatga tcgcgatatt gcgttgacac
    10321 aaggggaagt taaatgaaaa cagaacgtat tctcggtgct ctttatgggc aggcgttagg
    10381 ggatgcgatg gggatgccct ccgagctttg gccacgcagc cgcgttaaag cacactttgg
    10441 ctggattgac cgttttctgc ctggaccaaa ggagaataac gcggcctgtt attttaaccg
    10501 cgccgaattc accgacgata cctcgatggc gctgtgtctg gcggatgcgt tactggaacg
    10561 tgaaggcaag atcgatccgg atctgattgg gcgtaatatt ctcgactggg cgctgcgttt
    10621 cgacgccttt aacaaaaacg tactaggtcc gacctcgaag attgcgctta acgccattcg
    10681 cgacggtaaa cccgttgctg aactggaaaa caacggcgtg accaacggcg cagcgatgcg
    10741 cgtctcgcca ttaggttgtt tgcttccggc gcgtgatgtt gattccttta ttgatgatgt
    10801 ggcgctggcg tcgagcccga cccataaatc cgatctggca gttgcaggtg cggtagtcat
    10861 cgcatgggcg atttctcgtg ccattgacgg agaaagctgg tcagcgattg ttgattccct
    10921 gccttcaatt gcgcgacatg cacagcaaaa acgcatcact accttcagcg cctcactggc
    10981 agcacgtctg gagattgcgc tgaaaattgt gcgcaatgcc gacggcaccg aatccgccag
    11041 cgaacagctt taccaggtcg ttggcgcagg taccagcact attgagtccg ttccgtgcgc
    11101 cattgcgctg gttgaactgg cacaaaccga cccgaatcgc tgcgccgtcc tgtgcgctaa
    11161 ccttggcggc gacacagaca ccatcggcgc tatggcgacg gcgatttgcg gcgcgttgca
    11221 tggcgttaac gctatcgatc ctgcattaaa ggcggaactg gatgcggtaa atcagcttga
    11281 tttcaaccgc tatgccacag cgctggcgaa atatcgtcaa caacgggagg cggtatgagc
    11341 ggcgctcgat tacacacgct gctgcctgaa ttaacctcgc gtcagccggt gatggtcgtc
    11401 ggcgcggcgg tcattgatgt gatcgccgac gcttatgccc tcccctggcg tgggtgcgat
    11461 atcgaactga agcagcagag cgttaacgtt ggcggctgcg cactgaatat tgccgtggcg
    11521 ttaaagcgcc tcggcatcga agcgggtaat gccttgccgc tcggtcaggg cgtgtgggcg
    11581 gagattattc gcaaccggat ggcgaaagag gggttaatca gcctgatcga taacgccgaa
    11641 ggcgataacg gctggtgtct ggcgctggtt gagccggatg gcgaacgcac ttttatgtca
    11701 ttcagcggtg tggaaaatca gtggaatcgc cagtggctgg cgcgattaac cgttgcgcct
    11761 ggcagcctgc tctatttttc cggttatcaa ctggcctcgc cctgcggcga attgttagtg
    11821 gaatggctgg aaaagctgca agacgtgacg ccgtttatcg attttggccc acgtatcggc
    11881 gatatcccgg acgcattact ggcgcggatc atggcctgtc gacctttagt atcactcaat
    11941 cgtcaagagg ctgagattgc cgccgaacgt tttgctttat ccgcagagat aacaacactt
    12001 ggcaagcaat ggcaggagaa atttgccgcg ccgttgatcg ttcgtctcga taaagagggt
    12061 gcatggtatt tcagcaacga cgcttctggc tgcattccgg catttccaac gcaagttgta
    12121 gacaccattg gggcgggcga cagtcatgca ggtggcgtac ttgccgggct ggcctctggt
    12181 ctgccactgg cggatgccgt attactgggc aatgcagtgg cgtcgtgggt tgtcgggcat
    12241 cggggcggtg attgtgcgcc aacgcgcgag gaactactcc tcgcacacaa aaacgtatag
    12301 atcgctgcga cagtggctaa tgctgtactc aataggccgt tgctgttggt caagcgccac
    12361 ttgcttgatc accagcaccg gtattttgct atctaactga atatgtgact gaaactcggc
    12421 atccggcatc cgggcgctaa cgcgggaacg cgtacgttgt gggtaaatgt gctggctgcg
    12481 gaaatagtca taaagtgaaa tcccgatggc atcaacatca tgaattaaat gcgccggaac
    12541 ccacgattcc tcaatcgata ccgcttcttc atcgacatag cgaatacgct taagcaaaaa
    12601 gacatcactt cctgcttcga ccgccagctg ctgcgcgact tcttcgggac atttcacgac
    12661 acgcttgtta acccatagcg tatcgggctt tttcccgcgc aataccacct gctgagaaaa
    12721 acctcgcgct tctttcaacg agtattcgaa gatgttgttg atctgcgtgc cgtaaccgcg
    12781 cgaacgcgtc accacaccct cttcttccag cgcctgcatc gccttgcgca ccgtaatgcg
    12841 cgaaacgccg gttaactgac ttaaatcacg ctcaccgggc aaaatattgc catgctccag
    12901 caccccgctg cgcacggcat tttttaccgt ttcggcaaac ttaatataaa gcggcgtgtt
    12961 atccgccgct aaaatacgct cgttcagttg ggcaatcagc tgggtatgcg cttgttccat
    13021 ttgtcttttt cctgccgggg agtctggtgg gagtatatca ctgcttctta tttaaggcgt
    13081 ttctttaatc ccatcaacga atgcctgcaa ctcctccacc gtgccatgaa atacattaaa
    13141 atccaccgga ccattgatgc catctacctg tccacggtcg gaatgctgcc agaagcgcca
    13201 ggccatgccg tcattgtccg gacgacgttg ataatagtga gccacccacc acggatattc
    13261 attgaaatag cccgccagat tggtgtgata aaaaacggct cctgagtaaa taatcggctt
    13321 ttttcccgta cttttttcga ccatttttag ccactgactt actcgtttgc gtaattcttt
    13381 tgccgataat tttccccgtt cttctacgtc cagtaccgca gggaaatcgc cttgtgagaa
    13441 atccaccgtt tgcagaaata atcgtgcctg aaccggcgct gctaccgacg gggagaaata
    13501 atgatacgcc ccgcgcagca ggccattttc gcggcttagt tgccagttac gcgaaaaata
    13561 cgggtccacc agcttttcgc cttccgtcgc cttaataaaa gcaaactgta agcggatacc
    13621 gttgtcgcgc atttttgcca cccgctgcca gtcgatccgc tcctgccagc gagaaacatc
    13681 tattccgtga atggtgtaac tggcaggaat gcgaatagcg aaagatttaa ccgggcgata
    13741 accgtagaaa tggatgtagt ctgtaatctg aactgctgtc gaatagaaaa aattcaccgt
    13801 ctgacgcgga taaattgcaa cgatggaaat tagcccaaga gcgcataata gagaggtgag
    13861 tttttttcga ctggtaattc ttagctgcat tggtttcatc cctgaatgtc agtgccaaag
    13921 gctgacaata accaaagcag ctatagtacg gtgctcacca ccaggcgtgg aagtggtgaa
    13981 ccggaccaat accgtgacca acttccagcg tgtcggcctg ggctaatgct gatgaaagcc
    14041 agctttttgc ctcctgtacg gtgtcagccc agtttgtatg gcgcgggcgt agtgcagcca
    14101 acgccgcaga gagcgtacaa ccagtgccgt gggtgttttt ggtcataatg cgcggtgcgg
    14161 taaaccgttg ttcaccctcg cgggtaaaca gccagtccgg gctttgctca tcatccagat
    14221 gaccaccttt cattagcact gcgccacagc ccatcgccaa cagcgatcgc ccttgttcca
    14281 gcatttcctg ttcggtgcgc gcgtgtggcg cgtcgagcaa ggcggcagct tcgggcaagt
    14341 ttggcgttat taatgaaacc tgtggcaata atcgactgcg cagcgtagca accgccgaag
    14401 gtgaaagcag cgggtcgccg ctttttgcca gcataacggt gtcgagtacc acgttttgga
    14461 tctgataacg ttgcaaccgt tctgccaccg cttcaacaat atcggtttcc gccaacatac
    14521 cgattttagt ggtgtcgatt cgcacatcgc tgaacaccga atcgagctgg gcggcgacaa
    14581 aatcaggctc aatgcgatac accgactgta cgccacgggt attttgcgcc accagtgcag
    14641 taataactga gcaaccataa gcgccaagtg ccgagaaggt tttaagatcg gcctgaatcc
    14701 ccgcaccacc actgggatca gtaccggcaa tcgtcagagc gttaattcgt ttcatgcctg
    14761 cacctcctgc gtcaattgcc agagcgcatc aaggaaatgt ggaacaaaac tgcctggccc
    14821 ctcgcttctg gcgactgcgc gctctccggc ttgtttcatc cagtgacagg cagatgcgac
    14881 attttccagc gtatcgcctg gtaacgcaca gcaggcagcg acaaccgccg ataatgcaca
    14941 gccagttcct accactttgg tcattaacgg atcgccaccg tgaatgccaa cggcgcgatg
    15001 gccatcggta acataatcca tctcgccagt gaccacgacg attgcgccag tttcccgtgc
    15061 cagtgtttgt gcagcgggta tcgcgttagc tgcggcgtca gtggtatcca ctccccgtcc
    15121 accattagca atgccagcta atgccatgat ttccgaagca ttaccacgta tggcggttgg
    15181 cttatgagac agaagttcca gacaaaaacg gcggcgataa tcgagtgcac caaccgctac
    15241 tggatcaagc gtccagggtg tttgagagct ttttgcttgc tcaacggcgg cacacatcga
    15301 ctgtgcgcgt agctgcgtca gcgtgccaac gttaatcagc aacgcactgg caatagccgc
    15361 aaactgactg gcctcttcgg tttcgataac catcgctggc gatgcaccga gcgccagcaa
    15421 ggtattggca gtaaaggttt gcaccacatc gttggtcatg cagtgcacaa gaggggaatg
    15481 ttggtgaaaa aggtgtaacg cgtgcgcaga ttgcgctgaa ctcagcaggt cgacttgcat
    15541 agtttgctcc tgccataacg tgaagaagca atgacctggt ggtccgtgac ttccctacgc
    15601 tggcattatc cagatcaggt gatacgggta tttctcagcc ttcacgcaga agggcacccc
    15661 gagtcgtttg gttgcgatga taaatcgcag aggaggatgg taatgtccag cgcacgcgtt
    15721 gtaaacgagg aaaaacggca gttttacaat cgcgttaata aattatttga tatatgaatc
    15781 cagcaccttc agaacgacat ccagatcttc ttcacgtttt agctcatccc cctggtgaac
    15841 gatgtgttcc gtcagatgac ctttaatcac ttcccgcatc agaccgttta ccgcgccacg
    15901 gatagcagca atctgttgta aaactgcagc gcattcgtgc ggttcgtcga gcattttctt
    15961 gagcgccacg acctggccct gaatcttact ggcacgcgct ttcagtttct gtttatcacg
    16021 gattgtatga gacatggcaa cacctggtta acaagaatat gaaaaatcat agcactatta
    16081 atctactggg gggtagtatc aggtactggg gggga
//
LOCUS       DAZGJW010000105        16045 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZGJW010000105 DAZGJW010000000
VERSION     DAZGJW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.105"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..110)
                     /gene="yojO"
                     /locus_tag="ACHKIK_003421"
     CDS             complement(<1..110)
                     /gene="yojO"
                     /locus_tag="ACHKIK_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO9084532.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIY"
     gene            219..713
                     /gene="napF"
                     /locus_tag="ACHKIK_003422"
     CDS             219..713
                     /gene="napF"
                     /locus_tag="ACHKIK_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO9084533.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            703..966
                     /gene="napD"
                     /locus_tag="ACHKIK_003423"
     CDS             703..966
                     /gene="napD"
                     /locus_tag="ACHKIK_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO9084534.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            963..3449
                     /gene="napA"
                     /locus_tag="ACHKIK_003424"
     CDS             963..3449
                     /gene="napA"
                     /locus_tag="ACHKIK_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HFO9084535.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3456..4151
                     /gene="napG"
                     /locus_tag="ACHKIK_003425"
     CDS             3456..4151
                     /gene="napG"
                     /locus_tag="ACHKIK_003425"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO9084536.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4138..5001
                     /gene="napH"
                     /locus_tag="ACHKIK_003426"
     CDS             4138..5001
                     /gene="napH"
                     /locus_tag="ACHKIK_003426"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO9084537.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGYICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4998..5447
                     /gene="napB"
                     /locus_tag="ACHKIK_003427"
     CDS             4998..5447
                     /gene="napB"
                     /locus_tag="ACHKIK_003427"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO9084538.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5457..6059
                     /gene="napC"
                     /locus_tag="ACHKIK_003428"
     CDS             5457..6059
                     /gene="napC"
                     /locus_tag="ACHKIK_003428"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO9084539.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6072..6695
                     /gene="ccmA"
                     /locus_tag="ACHKIK_003429"
     CDS             6072..6695
                     /gene="ccmA"
                     /locus_tag="ACHKIK_003429"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO9084540.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6692..7354
                     /gene="ccmB"
                     /locus_tag="ACHKIK_003430"
     CDS             6692..7354
                     /gene="ccmB"
                     /locus_tag="ACHKIK_003430"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO9084541.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VSGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7396..8133
                     /gene="ccmC"
                     /locus_tag="ACHKIK_003431"
     CDS             7396..8133
                     /gene="ccmC"
                     /locus_tag="ACHKIK_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO9084542.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8130..8339
                     /gene="ccmD"
                     /locus_tag="ACHKIK_003432"
     CDS             8130..8339
                     /gene="ccmD"
                     /locus_tag="ACHKIK_003432"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO9084543.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8336..8815
                     /gene="ccmE"
                     /locus_tag="ACHKIK_003433"
     CDS             8336..8815
                     /gene="ccmE"
                     /locus_tag="ACHKIK_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO9084544.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8812..10755
                     /gene="ccmF"
                     /locus_tag="ACHKIK_003434"
     CDS             8812..10755
                     /gene="ccmF"
                     /locus_tag="ACHKIK_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO9084545.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10752..11309
                     /gene="dsbE"
                     /locus_tag="ACHKIK_003435"
     CDS             10752..11309
                     /gene="dsbE"
                     /locus_tag="ACHKIK_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO9084546.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11306..12358
                     /gene="ccmH"
                     /locus_tag="ACHKIK_003436"
     CDS             11306..12358
                     /gene="ccmH"
                     /locus_tag="ACHKIK_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO9084547.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQENK"
     gene            complement(12393..13040)
                     /gene="narP"
                     /locus_tag="ACHKIK_003437"
     CDS             complement(12393..13040)
                     /gene="narP"
                     /locus_tag="ACHKIK_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO9084548.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13360..15951
                     /gene="yejO"
                     /locus_tag="ACHKIK_003438"
                     /pseudo
     CDS             13360..15951
                     /gene="yejO"
                     /locus_tag="ACHKIK_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT         3570 a         3941 c         4671 g         3863 t
ORIGIN      
        1 atataaatat gtttataacc atttgaaatg tgagcaaaag cccgtttttg ccacgcttcc
       61 cgccactctt ttgatcctgc tacaggtttt accccgatcg gggtatgcat ctttgacaca
      121 tcctttaata tcttagcggc tataaaaatg gcttattaat tatgcggttt atttggtcgc
      181 tctcaatttt cagagcgcgt taatgatgga aggtcaatgt gaagattgat gcatcccgtc
      241 ggggcatact cactggtcgc tggcgcaaag ccagtaacgg tatccgtccg ccctggtccg
      301 gtgatgaatc tcattttctg acccattgca cccgctgcga cgcctgtatt aatgcttgtg
      361 aaaacaacat tctgcaacgc ggcgcgggcg gctatccgag cgttaatttc aaaaataacg
      421 agtgcagctt ctgttacgcc tgcgcgcagg cttgccccga atcgctattt tctccgcgcc
      481 acaccagggc ctgggatttg cagttcacca ttggggacgc gtgcctggcg tatcagtcag
      541 ttgaatgccg ccgctgtcag gatagctgtg agccaatggc aattatcttt cgcccgacgc
      601 tctccgggat ctaccagccg caacttaata gccaactttg caacggatgt ggagcatgtg
      661 cagccagctg tccggtatca gccataactg cggagtatct ccatgcacac taactggcaa
      721 gtttgcagcc tggtcgtgca ggccaaaagc gaacgaattt cagacatcag cacccaactg
      781 aacgcctttc ccggctgtga agttgctgtc agcgacgcgc cgagcggtca gttgattgtg
      841 gtggtggaag cagaagacag cgaaacgctg atccaaacca ttgagtcagt acgcaacgta
      901 gagggcgtgc tggcggtgtc gctggtttat caccagcagg aagagcaagg tgaggaaaca
      961 ccatgaaact cagtcgtcgt agctttatga aagctaacgc cgttgcggcc gctgcggcgg
     1021 ctgccggtct cagcgtgccg ggcgttgccc gcgccgttgt tggtcagcag gaagccatta
     1081 aatgggataa agcgccgtgc cgtttctgcg gtactggttg cggcgttctg gtcggaacgc
     1141 agcaggggcg tgtggtggcc tgtcagggcg acccggacgc accggttaac cgtggcctga
     1201 actgcattaa gggctatttc ctgcccaaga tcatgtacgg taaagaccgt ttgacgcagc
     1261 cgctgctgcg tatgaaaaac ggtaaatatg acaaagaagg cgaatttacc ccaatcacct
     1321 gggatcaggc cttcgatgtg atggaagaga aattcaaaac cgccctgaaa gaaaaaggac
     1381 cggaatcgat cggtatgttc ggttctggtc agtggactat ctgggaaggt tatgccgcgt
     1441 ccaagctgtt caaagcgggc ttccgttcga acaacatcga cccgaacgca cgtcactgta
     1501 tggcgtcggc agtagttggc tttatgcgta cctttggtat ggatgagccg atgggctgct
     1561 atgacgacat cgagcaggct gacgcgtttg tgctgtgggg cgctaacatg gcggagatgc
     1621 acccgatcct ctggtcacgc atcactaacc gtcgtctctc taaccagaac gtcaccgtgg
     1681 cggtgctttc tacctaccag catcgtagct tcgagctggc ggataacggc atcatcttta
     1741 cgccacagtc tgacctggtg atcctgaact acatcgccaa ctatatcatt caaaacaatg
     1801 cgataaatca ggacttcttc agcaagcacg ttaacctgcg caaaggggcg acggacatcg
     1861 gctacggttt acgtccgacc catccgctgg aaaaagcagc gaagaatccg ggttctgacg
     1921 cctccgaacc gatgagcttt gaagattaca aagccttcgt tgccgagtat acgctggaaa
     1981 aaactgccga aatgaccggc gtgccgaaag accagttaga acaactggcg cagctgtatg
     2041 ccgatccgaa caagaaagtc atctcctact ggacgatggg cttcaaccag catactcgtg
     2101 gcgtgtgggc caacaacctg gtctacaacc tgcacctgct gaccggcaaa atttcccagc
     2161 cgggttgcgg tccgttctcc ctgaccgggc agccttccgc gtgtggtact gcgcgtgaag
     2221 tgggcacctt tgctcaccgt ctgcctgcgg acatggtggt gactaacgag aaacaccgtg
     2281 atatttgcga gaagaagtgg aatatcccga gcggcaccat tccggcgaaa atcggtctgc
     2341 acgcggtggc acaagaccgt gcactgaaag acggcaagct gaatgtttac tggaccatgt
     2401 gtaccaacaa catgcaggcc gggccgaaca ttaatgaaga gcgtatgccg ggctggcgcg
     2461 atccgcgcaa cttcatcatc gtctccgatc cgtatccgac agtcagtgcg ctggcagccg
     2521 acttgatcct gccgaccgca atgtgggtag agaaagaggg cgcttacggt aacgccgaac
     2581 gccgtactca gttctggcgt cagcaggtac aggcgccagg cgaagcgaaa tcggatctct
     2641 ggcagttagt ccagttctcc cgccgcttca aaactgaaga agtatggccg gaagagctgc
     2701 tggcgaagaa accggaactg cgtggcaaaa cgctgtacga agttctgtat gccacccccg
     2761 aagtgagcaa ataccctgta tccgaactgg cggaagatca gctgaacgat gaatcccgcg
     2821 agctgggctt ctatctgcaa aaagggctgt tcgaagagta cgcatggttt ggtcgcggtc
     2881 acggtcacga tctggcaccg ttcgatgact accacaaagc gcgcggtctg cgctggccgg
     2941 tggtgaacgg taaagaaacg cagtggcgtt acagcgaagg taacgacccg tacgtgaaag
     3001 cgggcgaagg ctataagttc tacggtaaac cggatggcaa agcggtgatc ttcgcactgc
     3061 cgttcgaacc ggcggcggaa gcaccggatg aagagtacga cctgtggctc tctaccggac
     3121 gcgttctgga gcactggcac accggcagta tgactcgccg tgtgccggaa ctgcaccgcg
     3181 ccttcccgga agcggtcttg tttattcacc cgctggatgc gaaagcgcgc gatctgcgcc
     3241 gtggcgacaa agtgaaagtg gtttctcgcc gtggcgaagt gatctcgatt gttgaaacgc
     3301 gcggtcgtaa ccgtccgcca cagggcctgg tgtacatgcc gttcttcgac gccgcacagc
     3361 tggttaacaa actgacgctg gatgcgaccg atccgctctc gaaagagacg gacttcaaga
     3421 agtgcgcagt caaactggag aaggtgtaag ccattatgtc ccggtcagcg aaacctcaaa
     3481 atggtcgccg ccgctttctg cgcgatgtcg ttcgcacagc aggcgggctg gctgccgtgg
     3541 gtgtggcgct ggggttacaa cagcaaaccg cacgcgcatc tggcgtgcgg ttgcgcccgc
     3601 ccggagccat aaacgagaac gcctttgcca gtgcctgtgt gcgttgcggt cagtgtgttc
     3661 aggcttgccc ttacgacacc ttaaaactgg cgacgctggc ctctggtctg tcggcgggca
     3721 cgccatattt tgtcgcacgg gatattcctt gcgaaatgtg tgaggacatt ccgtgcgcca
     3781 aagtgtgccc aagcggtgcg ctggatcgtg agattgaatc gatcgacgac gcgcggatgg
     3841 ggctggcggt actggtggac caggaaaact gtctcaactt tcaggggctg cgctgcgatg
     3901 tttgttatcg cgaatgcccg aaaattgatg aggccatcac cctggagctg gagcgcaaca
     3961 cgcgtaccgg taagcacgcc cgctttctgc cgacggttca cagcgacgcc tgtaccggtt
     4021 gcggtaagtg cgaaaaagta tgcgtgctgg aacaaccggc aatcaaggtg ttaccgctgt
     4081 cactggcgaa aggggagtta ggtcaccatt accgcttcgg ttggctggag gggaacaatg
     4141 gcaaatcgta aacgtgacgc cgggcgcgag gcgctggaga aaaaaggctg gtggcgcagt
     4201 caccgttggc tggtgttacg tcgcctttgt cagttcttcg tgctggggat gtttttgagc
     4261 ggtccatggt ttggtgtgtg gatcttgcac ggcaactaca gcagtagcct gttattcgac
     4321 accgttccgc tgaccgatcc gctgatgacg ctgcaaagtc ttgccagtgg tcatctgcct
     4381 gccacggtgg cgttgaccgg tgcggtgatt atcaccgtgc tctacgccct ggcgggaaag
     4441 cgattatttt gcagctgggt ctgcccgctg aacccgatta ccgacttagc gaactggtta
     4501 cgcaggcgct ttgacctcaa tcagtctgcg acgatccctc gccacattcg ctacgtgctg
     4561 ttggtggtca ttctgctggg atcggcctta actggcacgc tcatctggga atggatcaac
     4621 cccgtatctc tgatggggcg cagcctggta atgggcttcg gcagcggcgc gctgcttatt
     4681 ctcgcgctgt ttttatttga tctactggtc gttgaacacg gctggtgcgg gtacatttgt
     4741 ccggttggcg cgctgtatgg cgtgctgggt agcaaaggtg tgattaccgt tgcggccacc
     4801 gatcggcaga aatgtaaccg ctgtatggat tgttttcatg tttgcccgga accgcatgtg
     4861 ctacgtgccc cggtgctgga tgaacaaagc ccggtgcagg tcaccagccg cgattgcatg
     4921 acgtgcggtc gctgcgtgga tgtctgttct gaggatgtat ttacaataac tacacgatgg
     4981 agttcgggag cgaaatcatg aaaagccatg acctgaagaa agcgctgtgt caatggacgg
     5041 cgatgctggc cctggtggta agcggcgcgg tttgggcggc taatggcgtc gattttagcc
     5101 agtcgccgga agtatccggg acacaggaag gggccattcg gatgccaaaa gagcaggaac
     5161 ggatgccgct gaactatgtg aatcagccgc caatgatccc gcatagcgtt gaaggttatc
     5221 aggtaacgac caataccaac cgctgcttgc agtgccacgg tgtcgaaagt tatcgcacca
     5281 ctggcgcgcc gcgtatcagt cctactcact ttatggacag cgacggcaaa gtgggcgcgg
     5341 aagtggctcc gcgtcgttat ttctgtctgc aatgtcacgt accgcaggcc gataccgcgc
     5401 caatcgtggg gaataccttt accccatcaa aaggttacgg gaaataagag gttattatgg
     5461 gaaattctga ccgtaagcct ggtctgatta agcgcctgtg gaaatggtgg cgtaccccca
     5521 gccgtctggc gctggggacg ctgctgttga tcggttttgt tggcggcatc gtcttctggg
     5581 gtggctttaa caccgggatg gaaaaagcca ataccgaaga gttctgcatt agctgccacg
     5641 aaatgcgcaa cacggtgtat caggaataca tggattccgt gcactacaac aaccgtagcg
     5701 gcgtccgtgc gacctgtccg gattgtcacg ttccgcacga gtttgtgccg aagatgatac
     5761 gcaagctcaa agcaagtaaa gagctgtatg gtaaaatttt tggcgttatt gacacgccgc
     5821 agaaatttga agctcatcgt ctgacgatgg cacagaatga gtggcggcgc atgaaggaca
     5881 ataactcgca ggagtgccgt aactgtcaca acttcgagta tatggataca accgcccaga
     5941 aatcggttgc cgcgaagatg catgaccagg cggtgaaaga tgggcaaacc tgtattgatt
     6001 gccataaagg gatagcgcac aagctgcccg atatgcgtga agtcgagcca ggtttttaac
     6061 agggttattg cgtgggtatg cttgaagcca gagagttact ttgtgagcgg gatgaacgaa
     6121 ccttatttag tggcttgtca tttacgctga acgcaggaga gtgggtacaa atcaccggta
     6181 gcaacggcgc ggggaagaca acgcttctcc gtttgctgac ggggttgtct cgccctgacg
     6241 caggcgaggt tctctggcaa gggcagccct tgcatcaggt acgcgacagc taccatcaaa
     6301 acctgttatg gataggccat cagccgggga tcaaaacccg gctgacggcg ttagaaaatc
     6361 tgcactttta tcatcgcgat ggcgataccg cacaatgtct ggaagccctg gcgcaggccg
     6421 ggcttgccgg attcgaagat attcctgtaa atcagctctc ggccgggcaa caacgccgcg
     6481 tcgctttagc gcgtctgtgg ctgacccgtg ccacgttatg gatcctcgac gagcctttta
     6541 ccgcgattga cgttaacggc gtcgatcgtc tgacccagcg tatggcgcag catacggagc
     6601 agggggggat tgtgattctg actacccacc agccgctcaa cgttgctgaa agtaaaattc
     6661 gccgcatttc actgacgcaa acgagggccg catgatgttc tggcgcattt tccgtcttga
     6721 gctgcgtgta gcgtttcgcc atagcgccga aatcgccaac ccgctgtggt tcttcctgat
     6781 tgtaattacc ctttttccgc tcagtatcgg tccggagccg caactgctgg cgcgtattgc
     6841 accgggcatt atctgggttg ctgcactgct ttcatccttg ctggcgctgg aacgactgtt
     6901 ccgtgacgat ttgcaggacg gcagtcttga acaattgatg ttgttgccgt tacccttgcc
     6961 cgccgttgtg ctggcgaagg tgatggcgca ctggatggta agcggtctgc cgttactcat
     7021 tctttcgcca ctggtagcaa tgctactggg aatggatgtt tatggctggc aagtgatggc
     7081 gctgacgctg ctgctgggaa cgcctacgct tggctttctc ggtgcaccgg gcgtggcgct
     7141 gacagtggga cttaagcgcg gtggtgtgct gctcagcata ctggtgttac cgctgactat
     7201 cccattactc atcttcgcca ccgccgcgat ggacgcggct tccatgcatt tgcccgttga
     7261 cgggtatctg gcaattttag gcgcgttgct ggcaggcacc gcgacattaa gtccttttgc
     7321 gacggcggca gcgttacgaa tcagcattca ataacgctgg attactgatt tttgttcgag
     7381 tctggtatcg aaactatgtg gaaaacactg catcaactgg cgatcccacc acggctgtat
     7441 caaatctgtg gctggtttat accgtggctg gcaattgcca gtgtggtcgt gcttaccgtc
     7501 ggctggatct ggggattcgg ctttgctccg gctgattatc agcagggaaa tagctaccgc
     7561 attatctacc tgcatgtgcc tgcggcgatc tggtcgatgg gcatttatgc atcaatggca
     7621 gtggcagcgt ttattggcct tgtctggcag atgaaaatgg ccaacctggc ggtggcggcg
     7681 atggccccca ttggtgccgt gtttaccttt attgccctgg ttaccggctc tgcatgggga
     7741 aaaccgatgt ggggcacctg gtgggtatgg gatgcacgtc tgacttctga actggtgctg
     7801 ctttttttgt atgtgggtgt gattgccctg tggcacgcct tcgacgaccg ccgtctggcg
     7861 ggccgtgcgg caggtatcct ggtgctgatt ggcgtggtga atctgccgat tattcattac
     7921 tccgtggagt ggtggaacac cctgcatcag ggatcaacgc ggatgcagca aagtatcgat
     7981 ccggcgatgc gttcgccgct gcgctggtcg atttttggct tcctgctcct gtctgccacg
     8041 ctgacgctga tgcggatgcg taatttgatt ttgctgatgg aaaaacgccg tccgtgggtg
     8101 agtgaactga tactgaaaag aggccgtaaa tgacccctgc atttgcttcc tggaatgaat
     8161 ttttcgcaat gggcggttac gccttttttg tctggctggc ggtggtgatg accgttattc
     8221 cgctggtggt tttggtcgtg cactcggtga tgcagcatcg cgcaattctg cgtggtgtgg
     8281 cgcaacagcg ggcgcgtgag gcgcgtttac gtgctgcgca acagcaggag gctgcatgaa
     8341 tattcgccgt aaaaaccgct tgtggattgc ctgtgctgtt ctggcagggt tggcactgac
     8401 tatcggtctg gtgctatatg cgctgcgctc gaatatcgat ctcttttata cgccggggga
     8461 aattctctac ggcaagcgtg aaactcagca aatgccggaa gtcggtcagc gtctgcgcgt
     8521 tggcgggatg gtgatgccgg gtagcgtgca gcgcgatccc aattcgctaa aagtaacttt
     8581 caccatttac gacgctgaag gttcagtaga tgtctcttac gaaggcattt tgccggatct
     8641 gttccgtgaa gggcagggcg ttgtggtgca gggcgaactg gaaaaaggca atcatatcct
     8701 cgcaaaagaa gtgctggcga aacacgacga aaactacacg ccgccagaag ttgagaaagc
     8761 gatggaagcc aatcaccgtc gcccggcgag tgtttataag gacccagcat catgatgccg
     8821 gaaattggta acgggctgct gtgtctggcg ctaggaattg cgctgctgct gtccgtgtat
     8881 ccgctatggg gcgtagcgcg cggagatgcg cgcatgatgg cgtcttcccg cttgtttgcc
     8941 tggctgctgt ttatgtctgt ggctggcgca tttctggtac tggtcaatgc cttcgtggtc
     9001 aacgacttca ccgtcaccta tgttgccagc aactccaata cccagcttcc ggtgtggtat
     9061 cgcgtggcgg ctacctgggg cgcgcatgaa ggctcgctcc tgctgtgggt gctgctgatg
     9121 agcggctgga cctttgcggt agcgattttt agtcagcgta ttccgctgga tattgtggcc
     9181 cgcgtactgg cgataatggg gatggtcagc gtcggctttt tgctgttcat tctctttacc
     9241 tctaacccgt tctcacgcac gttgccgaac ttcccgattg aagggcgcga tcttaacccg
     9301 ctgttacagg atccggggct gatcttccat ccgcctctgc tctatatggg gtacgtgggg
     9361 ttctcggtgg cgtttgcttt tgccattgct tctttgttgt gcgggcgtct ggacagcact
     9421 tatgcgcgtt ttactcgtcc gtggacgctg gcggcgtgga tcttcttgac gctcggcatt
     9481 gtgctcggtt ccgcatgggc ctattacgaa ctcggctggg gcggctggtg gttctgggat
     9541 ccggtagaaa acgcctcgtt tatgccgtgg ctggtgggga ctgcgctgat gcactcactg
     9601 gcggtcactg aacaacgcgc cagcttcaaa gcgtggacat tactgctggc aatcagtgcc
     9661 ttctcgttgt gtctgctggg gactttcctg gtgcgttccg gcgtgctggt atcggtacac
     9721 gcgtttgcct ctgatccggc acgcggtatg tttatcctcg cctttatggt actggtgatt
     9781 ggtggttcgc tgctgctgtt tgccgcgcgt ggacacaaag ttcgctcacg cgtaaacaat
     9841 gcgctgtggt cgcgggaatc tctgctgtta gcgaacaacg ttttgctggt cgccgcgatg
     9901 ctggtggtat tactggggac gctgctgccg ctggtgcaca agcaactggg actgggcagt
     9961 atttcgattg gcgaaccgtt cttcaacacc atgtttacct ggctgatggt gccgtttgcg
    10021 ctgctgcttg gtgtcggtcc tctggtgcgc tgggggcgcg atcgcccacg taaaatccgc
    10081 aatttattga ttatcgcctt catctcaacg ctggtgctgt cgctgctgct gccgtggctg
    10141 ttcgaaagca aagttgtggc gatgacggtg ctcggcctgg caatggcctg ctggattgct
    10201 gtgctggcaa ttgcggaagc tgcgctgcgt atttcacgtg gcacgaaaac caccttcagt
    10261 tattggggaa tggtggcggc tcacctgggg ctggcagtga caattgttgg tattgccttt
    10321 agccagaact atagcgttga gcgtgatgtg cgcatgaagt ccggcgatag cgtcgatatt
    10381 catgaatatc gcttcacctt ccgtgatgtc aaagaggtga ctggcccgaa ctggcgtggc
    10441 ggtgtggcga ctatcggcgt aacgcgcgat ggcaagccgg aaacggtgct gtatgcggaa
    10501 aaacgttatt acaacactgc cgggtcgatg atgaccgaag cggcaattga cggcggcatc
    10561 acgcgtgacc tgtacgccgc gctcggtgaa gagctggaaa acggcgcgtg ggccgtgcgt
    10621 ctttactaca aaccatttgt tcgctggatt tgggcgggcg ggctgatgat ggcgttgggc
    10681 ggactactgt gtctgtttga tcctcgctat cgtaagcgcg tgagtccgca aaaaactgcg
    10741 ccggaggccg tatgaagcgc aaagtattgt taattccgtt gattatcttc ctggcgattg
    10801 ccgcggcgct gctgtggcag ctggcgcgta atgccgaagg ggatgatccg accaatctgg
    10861 aatcggcgct cattggcaag cctgtgccga agtttcgtct cgaatcactg gacaatccgg
    10921 ggcagtttta tcaggcggat gtgctgactc agggcaaacc agtactgctt aacgtctggg
    10981 cgacctggtg tccgacctgc cgtgcggaac atcaatatct gaatcagctt tcagcgcagg
    11041 gcattcgcgt ggtcggcatg aactataaag acgatcgcca gaaggcaatc agctggctga
    11101 aagagctggg caatccttac gcgctaagcc tgtttgatgg cgacggcatg ttagggctgg
    11161 atctcggtgt ctatggcgcg ccagaaacgt tccttattga cggcaacggc atcattcgct
    11221 atcgccatgc gggcgatctc aatcctcgcg tctgggaaga agagatcaag ccgctgtggg
    11281 agaaatacag taaggaggcc gcacaatgag gtttttattg ggcgtgctga tgctgatgat
    11341 ctccggctca gcgctggcga ccatcgacgt gttgcagttt aaagatgaag cacaggaaca
    11401 acagttccgt cagctcactg aagaactgcg ctgcccgaaa tgccagaata acagcattgc
    11461 cgattccaac tcgatgattg ccaccgacct gcgccagaaa gtgtatgaac tgatgcagga
    11521 aggtaaaagt aagaaagaga ttgtcgatta tatggtggcg cgttacggca acttcgtcac
    11581 ttacgatccg ccgttaacgc cgctgaccgt gctgctgtgg gtgctgccag tagtggctat
    11641 tggcattggc ggttgggtca tttacgcccg ctcgcggcgt cgggtacgcg tggtgccgga
    11701 agcgtttcct gaacaaagcg tgccggaagg taagcgtgcc ggatatgtcg cttatttgcc
    11761 gggcattgtg gtggcgttaa ttgtggctgg cgtcagctac taccagaccg gaaattatca
    11821 acaggtgaaa atctggcagc aggccacggc acaggctccg gcgttgctgg acagggcgct
    11881 ggatccgaaa gccgatccgc tcaacgaaga agagatgtcg cgtcttgcgc tggggatgcg
    11941 tactcaactg caaaaaaatc cgggagatat agaaggctgg attatgttgg gccgcgttgg
    12001 catggcgctg ggtaacgcta gtatcgccac cgatgcatac gctactgcgt atcgcctcga
    12061 tccgaaaaac agcgatgccg cactgggtta tgctgaagcg ttgacacgtt catctgatcc
    12121 caacgacaac cgcctcggcg gcgaactgct gcgccagttg gtgagaagcg accacagcaa
    12181 tatccgtgtg ttaagcatgt atgcgtttaa tgcctttgag cagcagcgat ttggcgaagc
    12241 cgttgccgcg tgggagatga tgttgaaact cttacctgcc aacgatactc gccgtgcggt
    12301 gattgaacgt agtatcgcgc aggcgatgca acatttgtcg ccgcaggaga ataaataaaa
    12361 agaggcccga tggtgatgcc atcgggctat ttttattgtg ccccgcgttg ttgcaggaac
    12421 agaatggtgg ctgccacgcg tgagcggaca ttgagtttgc gcagcagatt gcgaatatgt
    12481 acttttactg tctgctctga aatattcaac accgaggcaa tctgtttatt tgacagcccc
    12541 tgtgccagct cgtgcagaac atccagctcg cgctccgtca gcacgctgaa gggatcttct
    12601 tccgcgccaa acatttcacg ttcacgtaag tactgattga cgcgttcgct aaagactttg
    12661 ctgcctttcg ctccagcacg aattgcttcc agcaatactt ccgggtcgct gtctttcaac
    12721 agataaccgt ctgcgcctgc gtctatcagc gcaaagacat cgctggaggc atctgatacg
    12781 gtcaggataa taatttgcgc ggtaacgcca tccctgcgca aggcattgag ggtatccagg
    12841 ccactcatac ctttcatatt gagatccagc aagatcacgt cgatatccag tctattcgcc
    12901 agatcgatcg cgctcgcgcc gtcgcccgct tcggcgacca cttcaaagcc aggatcaagc
    12961 tccagtaact gacgaacacc gcgtcgcata agtggatgat catccacaat catcacctga
    13021 aaaggcgttg cttcaggcat agtagtctcc tgaggtttta ttagaatcat tatgtttgag
    13081 cgtagcgttg gtaacgctac caggcgcgtg aatgttaccc cattttatgt atggacatgt
    13141 agtgaaaact acaaccttgt cgttcgcttt ggcatcattt tcattataaa gacggaattt
    13201 tgcatcagct tctcatcaag aaaaacaact aaaaaatcaa tcacaatact tgtgcatttc
    13261 tttaaacagt ttttaaaaac tgactcccgg tatggagcta tgggtatttt ctgtacccaa
    13321 tgcttttaac agcaattaat ttcataggat gaaagctcaa tgcatcaatc tggttctgtt
    13381 tctctttgtc gttccgcaat atctgttctg gtggctacag cgttatattc acccatagca
    13441 ttggcatcaa cagttgagta tggtgagaca gttgatggtg ttgtcctgga aaaagatatc
    13501 cagctggttt atgggaccgc caataatacg aaaatcaatc ctggcggaga acagcatata
    13561 aaagaatttg gtgtaagtaa taatactgaa attaacggag ggtatcagta cattgaaatg
    13621 aatggcgccg cagaatactc agtattaaat gacggttatc aaattgttca aatgggtggc
    13681 gcggcaaacc agactacgct caataatggt gtgctacagg tttatggcgc agcgaatgat
    13741 accacgatta aaggcgggcg cttaatcgtt gaaaaagatg ggggggccgt ctttgtcgct
    13801 atcgaaaagg gaggactact ggaggttaaa gaggggggat tagcgattgc ggtagatcag
    13861 aaagcaggcg gtgctattaa aacaaccacg cgggccatgg aggtattcgg aacaaaccgt
    13921 ctcggtcagt tcgatatcaa gaatggtatt gctaataata tgttgttgga aaacggcgga
    13981 agtttgcgag ttgaagaaaa tgacttcgct tataatacca ctgtagatag tggcggctta
    14041 ctggaggtta tggatggcgg gactgtaact ggcgttgata aaaaagcagg cggaaaatta
    14101 attgtctcaa cgaatgcgct ggaagtgagt ggtccaaaca gtaaaggcca atttagtata
    14161 aaagatggtg tgtcaaaaaa ttatgaactg gatgatggtt ccgggctcat tgttatggag
    14221 gacacgcagg ccattgatac tatccttgat gagcatgcca ctatgcaatc gctgggaaag
    14281 gatactggta cgaaagtgca ggcaaatgcg gtatatgatc tcggtcgatc atatcagaat
    14341 ggaagtatca cgtattcctc aaaagccatc tctgaaaata tggttatcaa caatggccgc
    14401 gctaacgtct gggctggcac aatggttaac gtttcagtca gagggaatga tggcattctt
    14461 gaggtcatga agccgcaaat aaattatgca cccgcaatgt tggtgggtaa ggtagtggtt
    14521 tctgagggcg cttcttttag aacgcatagt gccgtggata ccagcaaagc ggacgtttcg
    14581 ctcgaaaata gcgtatggac catcattgcc gatatcacta cgacgaacca aaacaccctc
    14641 ctcaacttag ccaaccttgc gatgtctgac gcaaatgtga ttatgatgga tgagccagtg
    14701 actcgttcat cagtgacggc aagtgcggaa aatttcatta cgttgaccac caataccctg
    14761 tcgggaaacg gcaattttta tatgcgtacc gatatggcta atcatcagag cgatcagctc
    14821 aacgtcaccg gtcaggcaac aggtgatttc aaaatattcg tgacggacac cggtgccagc
    14881 ccggcagcag gagatagcct tacactggta acaacgggcg gcggtgatgc tgcatttacg
    14941 ttgggcaatg ccggaggcgt tgttgatatc ggtacgtatg aatatacctt gctggataat
    15001 ggcaaccata gctggagttt ggcagagaat cgcgcgcaaa ttaccccttc aaccactgat
    15061 gtgctgaata tggcggcagc acaaccgctg gtatttgatg cagaactgga caccgtgcgt
    15121 gagcgtcttg gtagcgtaaa aggcgttaat tacgatacgg cgatgtggag ttcggcaatt
    15181 aacacccgca acaacgtgac cactgatgcg ggcgctggct ttgagcaaac attgaccggg
    15241 ctgacgctcg gtatcgatag ccgtttctcc cgtgaagaaa gcagcacaat tcgcggcttg
    15301 ttctttggtt actctcattc tgatattggt tttgatcgcg gcggcaaagg caatgtcgat
    15361 agctataccc tgggggctta tgccggttgg gagcatcaga acggtgccta tgttgatgga
    15421 gtggtgaaag ttgaccgttt tgccaacacc atccatggca agatgagtaa tggggcaaca
    15481 gcgtttggcg attacaatag taacggcgcg ggtgctcatg tcgagagcgg gttccgttgg
    15541 gttgacggat tgtggagtgt tagaccctat ctggccttta ccggctttac cacagatggt
    15601 caggactaca cgttatcaaa cggcatgcgc gcggatgtgg gaaatacccg gatattacgc
    15661 gctgaagcgg gaacggcggt aagctatcac atggacctgc aaaacggtac gacgctggaa
    15721 ccctgactga aagccgccgt gcgtcaggaa tacgccgatt ctaaccaggt gaaagttaat
    15781 gacgatggca aatttaataa tgatgtggct ggaacccgtg gcgtttatca ggctgggata
    15841 aggtcatcgt ttaccccgac gttaagcggt catttgtcag tcagctatgg caatggcgca
    15901 ggggtagaat cgccgtggaa tactcaggcg ggtgtggtct ggacgttctg ataacaaaaa
    15961 ataaacaggc tgtgatgtgt cacggtctgt ttatcgaatt aattgcagat ataaaaaaaa
    16021 ccaaccgtaa aggttggttt tttct
//
LOCUS       DAZGJW010000106        15790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZGJW010000106 DAZGJW010000000
VERSION     DAZGJW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.106"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            276..680
                     /gene="sdhC"
                     /locus_tag="ACHKIK_003439"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             276..680
                     /gene="sdhC"
                     /locus_tag="ACHKIK_003439"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO9084549.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFI
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            674..1021
                     /gene="sdhD"
                     /locus_tag="ACHKIK_003440"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             674..1021
                     /gene="sdhD"
                     /locus_tag="ACHKIK_003440"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO9084550.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1021..2787
                     /gene="sdhA"
                     /locus_tag="ACHKIK_003441"
     CDS             1021..2787
                     /gene="sdhA"
                     /locus_tag="ACHKIK_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO9084551.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEVSLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2803..3519
                     /gene="sdhB"
                     /locus_tag="ACHKIK_003442"
     CDS             2803..3519
                     /gene="sdhB"
                     /locus_tag="ACHKIK_003442"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO9084552.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYDKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3820..6621
                     /gene="sucA"
                     /locus_tag="ACHKIK_003443"
     CDS             3820..6621
                     /gene="sucA"
                     /locus_tag="ACHKIK_003443"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO9084553.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6636..7853
                     /gene="odhB"
                     /locus_tag="ACHKIK_003444"
     CDS             6636..7853
                     /gene="odhB"
                     /locus_tag="ACHKIK_003444"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO9084554.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            7947..9113
                     /gene="sucC"
                     /locus_tag="ACHKIK_003445"
     CDS             7947..9113
                     /gene="sucC"
                     /locus_tag="ACHKIK_003445"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO9084555.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9113..9982
                     /gene="sucD"
                     /locus_tag="ACHKIK_003446"
     CDS             9113..9982
                     /gene="sucD"
                     /locus_tag="ACHKIK_003446"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO9084556.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10086..10808)
                     /gene="mngR"
                     /locus_tag="ACHKIK_003447"
     CDS             complement(10086..10808)
                     /gene="mngR"
                     /locus_tag="ACHKIK_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFO9084557.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            10977..12893
                     /gene="mngA"
                     /locus_tag="ACHKIK_003448"
     CDS             10977..12893
                     /gene="mngA"
                     /locus_tag="ACHKIK_003448"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFO9084558.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLGSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLMWRRH
                     AVKHGNYLTDGVMP"
     gene            12911..15544
                     /gene="mngB"
                     /locus_tag="ACHKIK_003449"
     CDS             12911..15544
                     /gene="mngB"
                     /locus_tag="ACHKIK_003449"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFO9084559.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YEMAVPRNLQERSARQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            15569..>15790
                     /locus_tag="ACHKIK_003450"
     CDS             15569..>15790
                     /locus_tag="ACHKIK_003450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084560.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELII"
BASE COUNT         3780 a         3969 c         4353 g         3688 t
ORIGIN      
        1 aggataaaac ccgacaaact atatgtaggt taattgtaat gattttgtga acagcctata
       61 ctgccgccag gtctccggaa caccctgcaa tcccgagcca cccagcgttg taacgtgtcg
      121 ttttcgcatc tggaagcagt gttttgcatg acgcgcagtt atagaaagga cgctgtctga
      181 cccgcaagca gaccggagga aggaaatccc gacgtctcca ggtaacagaa agttaacctc
      241 tgtgcccgta gtccccaggg aataataaga acagcatgtg ggcgttattc atgataagaa
      301 atgtgaaaaa acaaagacct gttaatctgg acctacagac catccggttc cccatcacgg
      361 cgatagcgtc cattctccat cgcgtttccg gtgtgatcac ctttattgca gtgggtatcc
      421 tgctgtggct tctgggtacc agcctctctt cccctgaagg tttcgagcaa gcttccgcga
      481 ttatgggcag cttcttcgtc aaatttatca tgtggggcat ccttaccgct ctggcgtatc
      541 acgtcgtcgt aggtattcgc cacatgatga tggattttgg ctatctggaa gaaacattcg
      601 aagcgggtaa acgctccgcc aaaatctcct ttgttattac tgtcgtgctt tcacttctcg
      661 caggagtcct cgtatggtaa gcaacgcctc cgcattagga cgcaatggcg tacatgattt
      721 catcctcgtt cgcgctaccg ctatcgtcct gacgctctac atcatttata tggtcggttt
      781 tttcgccacc agtggcgaac tgacatatga agtctggatc ggtttcttcg cctctgcgtt
      841 caccaaagtg ttcaccctgc tggcgctgtt ttctatcttg attcatgcct ggatcggcat
      901 gtggcaggtg ttgaccgact acgttaaacc gctggcattg cgcctgatgc tgcaactggt
      961 gattgtcgtt gcactggtgg tttacgtgat ttatggattc gttgtggtgt ggggtgtgtg
     1021 atgaaattgc cagtcagaga atttgatgca gttgtgattg gtgccggtgg cgcaggtatg
     1081 cgcgcggcgc tgcaaatttc ccagagcggc cagacctgtg cgctgctctc taaagtcttc
     1141 ccgacccgtt cccataccgt ttctgcgcaa ggcggcatta ccgttgcgct gggtaatacc
     1201 catgaagata actgggaatg gcatatgtac gacaccgtaa aagggtcgga ctatatcggt
     1261 gaccaggacg cgattgaata tatgtgtaaa accgggccgg aagcgattct ggaactcgaa
     1321 cacatgggcc tgccgttctc gcgtcttgat gatggtcgta tctatcaacg tccgtttggc
     1381 ggtcagtcga aaaacttcgg cggcgagcag gcggcacgca ctgcggcggc ggctgaccgt
     1441 accggtcacg cactgttgca cacgctttat cagcagaacc tgaaaaacca caccaccatt
     1501 ttctccgagt ggtatgcgct ggatctggtg aaaaaccagg atggcgcggt ggtgggttgt
     1561 accgcactgt gcatcgaaac cggtgaagtg gtttatttca aagcccgcgc taccgtgctg
     1621 gcgactggcg gggcagggcg tatttatcag tccaccacta acgcccacat taacactggc
     1681 gacggtgtcg gcatggctat ccgcgcaggc gtaccggtgc aggatatgga aatgtggcaa
     1741 ttccacccga ccggcattgc cggtgcgggc gtactggtca ccgaaggttg ccgtggtgaa
     1801 ggcggttatc tgctgaacaa acatggcgaa cgctttatgg agcgttatgc gccgaacgcc
     1861 aaagacctgg cgggccgtga tgtggtggcg cgttccatca tgattgaaat ccgtgaaggt
     1921 cgcggctgtg atggtccgtg ggggccacac gcgaaactga aactcgatca cctgggtaaa
     1981 gaagttctcg aatcccgtct gccgggtatc ctggagcttt cccgtacctt cgctcacgtc
     2041 gatccggtga aagagccgat tccggttatc ccaacctgtc actacatgat gggcggtatt
     2101 ccgaccaaag ttaccggtca ggcgctgact gtgaatgaga aaggcgaaga tgtggttgtt
     2161 ccgggactgt ttgccgttgg tgaaatcgct tgtgtatcgg tacacggcgc taaccgtctg
     2221 ggcggcaact cgctgctgga cctggtggtc tttggtcgcg cggcaggtct gcatctgcaa
     2281 gagtctatcg ccgagcaggg cgcactgcgc gatgccagcg agtctgatgt tgaagtgtct
     2341 ctggatcgcc tgaaccgctg gaacaataat cgtaacggtg aagatccggt ggcgattcgt
     2401 aaagcgctgc aagaatgtat gcagcataac ttctcggtct tccgtgaagg tgatgcgatg
     2461 gcgaaagggc ttgagcagtt gaaagtgatc cgcgagcgtc tgaaaaatgc ccgtctggat
     2521 gacacttcca gcgagttcaa cacccagcgc gttgagtgcc tggaactgga taacctgatg
     2581 gaaacggcgt atgcaacggc tgtttctgcc aacttccgta ccgaaagccg tggcgcacat
     2641 agccgcttcg acttcccgga tcgtgatgat gaaaactggc tgtgccactc cctgtatctg
     2701 ccagagtcgg aatccatgac gcgccgaagc gtcaacatgg aaccgaaact gcgcccggca
     2761 ttcccgccga agattcgtac ttactaatgc ggagacagga aaatgagact cgagttttca
     2821 atttatcgct ataacccgga tgttgatgat gctccgcgta tgcaggatta caccctggaa
     2881 gcggaagaag gtcgcgacat gatgctgctg gatgcgctta tccagctgaa agagaaagat
     2941 cccagcctgt cgttccgccg ctcctgccgt gaaggtgtgt gcggttccga cggtctgaac
     3001 atgaacggca agaatggtct ggcctgtatt accccgattt cggcactcaa ccagccgggc
     3061 aagaagattg tgattcgccc gctgccaggt ttaccggtga tccgcgattt ggtggtagac
     3121 atgggacaat tctatgcgca atatgacaaa attaagcctt acctgttgaa taatggacaa
     3181 aatccgccag ctcgcgagca tttacagatg ccagagcagc gcgaaaaact cgacgggctg
     3241 tatgaatgta ttctctgcgc atgttgttca acctcttgtc cgtctttctg gtggaatccc
     3301 gataagttta tcggcccggc aggcttgtta gcggcatatc gtttcctgat cgatagccgt
     3361 gataccgaga ctgacagccg cctcgacggt ttgagcgatg cattcagcgt attccgctgt
     3421 cacagcatca tgaactgcgt cagtgtatgt ccgaaggggc tgaacccgac gcgcgccatc
     3481 ggccatatta agtcgatgtt gttgcaacgt aatgcgtaaa ccgtaggcct gataagacgc
     3541 gcaagcgtcg catcaggcaa ccagtgccgg atgcggcgtg aacgccttat ccggcctaca
     3601 agtcattacc cgtaggcctg ataagcgcag cgcatcaggc gtaacaaaga aatgcaggaa
     3661 atctttaaaa actgcccctg acactaagac agtttttaaa ggttccttcg cgagccacta
     3721 catagacaag agctcgcaag tgaaccccgg cacgcacatc actgtgcgtg gtagtatcca
     3781 cggcgaagta agcataaaaa agatgcttaa gggatcacga tgcagaacag cgctttgaaa
     3841 gcctggttgg actcttctta cctctctggc gcaaaccaga gctggataga acagctctat
     3901 gaagacttct taaccgatcc tgactcggtt gacgctaact ggcgttcgac gttccagcag
     3961 ttacctggta cgggagtcaa accggatcaa ttccactctc aaacgcgtga atatttccgc
     4021 cgcctggcga aagacgcttc acgttactct tcaacgatct ccgaccctga caccaatgtg
     4081 aagcaggtta aagtcctgca gctcattaac gcataccgct tccgtggtca ccagcatgcg
     4141 aatctcgatc cgctgggact gtggcagcaa gataaagtgg ccgatctgga tccgtctttc
     4201 cacgatctga ccgaagcaga cttccaggag accttcaacg tcggttcatt tgccagcggc
     4261 aaagaaacca tgaaactcgg cgagctgctg gaagccctca agcaaaccta ctgtggcccg
     4321 attggtgccg agtatatgca cattaccagc accgaagaaa aacgctggat ccaacagcgt
     4381 atcgagtctg gtcgcgcgac tttcaatagc gaagagaaaa aacgcttctt aagcgaactg
     4441 accgccgctg aaggccttga acgttacctc ggcgcaaaat tccctggcgc aaaacgcttc
     4501 tcgctggaag gcggtgacgc gttaatcccg atgcttaaag agatgatccg ccacgctggc
     4561 aacagcggca cccgcgaagt ggttcttggg atggcgcacc gtggtcgtct gaacgtgctg
     4621 gtgaacgtgc tgggtaaaaa accgcaagac ttgttcgacg agtttgccgg taaacataaa
     4681 gaacacctcg gcacgggtga cgtgaaatac cacatgggct tctcgtctga cttccagacc
     4741 gatggcggcc tggtgcacct ggcgctggcg tttaacccgt ctcaccttga gattgtaagc
     4801 ccggtagtta tcggttctgt tcgtgcccgt ctggacagac ttgatgagcc gagcagcaac
     4861 aaagtgctgc caatcaccat ccacggtgac gccgcagtga ccgggcaggg cgtggttcag
     4921 gaaactctga acatgtcgaa agcgcgtggt tatgaagttg gcggtacggt acgtatcgtt
     4981 atcaacaacc aggttggttt caccacctct aatccgctgg atgctcgttc tacaccgtac
     5041 tgtactgata tcggtaagat ggttcaggca ccgattttcc acgttaacgc ggatgatccg
     5101 gaagccgttg cctttgtgac ccgtctggcg ctcgatttcc gtaacacctt taaacgtgat
     5161 gtcttcatcg acctggtgtg ctaccgccgt cacggccaca acgaagccga cgagccgagc
     5221 gcaacccagc cgctgatgta tcagaaaatc aaaaaacatc cgacaccgcg caaaatctac
     5281 gctgacaagc tggagcagga aaaagtggcg acgctggaag atgccaccga gatggttaac
     5341 ctgtaccgcg atgcgctgga tgctggcgat tgcgtagtgg cagagtggcg tccgatgaac
     5401 atgcactctt tcacctggtc gccgtacctc aaccacgaat gggacgaaga gtacccgaac
     5461 aaagttgaga tgaagcgcct gcaggagctg gcgaaacgca tcagcacggt gccggaagca
     5521 gttgaaatgc agtctcgcgt tgccaagatt tatggcgatc gccaggcgat ggctgccggt
     5581 gagaaactgt tcgactgggg cggtgcggaa aacctcgctt acgccacgct ggttgacgaa
     5641 ggcattccgg ttcgcctgtc gggtgaagac tccggtcgcg gtaccttctt ccaccgccac
     5701 gcggtgatcc acaaccagtc taacggttcc acttacacgc cgttgcaaca catccataac
     5761 gggcagggcg cgttccgcgt ctgggactcc gtactgtctg aagaagcagt gctggcgttt
     5821 gaatacggtt atgccaccgc agaaccacgc actctgacca tctgggaagc gcagttcggt
     5881 gacttcgcca acggtgcgca ggtggttatc gaccagttca tctcttctgg cgaacagaaa
     5941 tggggccgga tgtgtggtct ggtgatgttg ctgccgcacg gttacgaagg gcaggggccg
     6001 gagcactcct ccgcgcgtct ggaacgttat ctgcaacttt gtgctgagca aaacatgcag
     6061 gtgtgcgtac cgtctacccc ggcacaggtt taccacatgc tgcgtcgtca ggcgctgcgc
     6121 gggatgcgtc gtccgctggt cgtgatgtcg ccgaaatccc tgctgcgtca tccgctggcg
     6181 gtttccagcc tcgaagaact ggcgaacggc accttcctgc cagccatcgg tgaaatcgac
     6241 gagcttgatc cgaagggcgt gaagcgcgta gtgatgtgtt ctggtaaggt ttattacgac
     6301 ctgctggaac agcgtcgtaa gaacaatcaa cacgatgtcg ccattgtgcg tatcgagcaa
     6361 ctctacccgt tcccgcataa agcgatgcag gaagtgttgc agcagtttgc tcacgtcaag
     6421 gattttgtct ggtgccagga agagccgctc aaccagggcg catggtactg cagccagcat
     6481 catttccgtg aagtgattcc gtttggggct tctctgcgtt atgcaggccg cccggcctcc
     6541 gcctctccgg cggtagggta tatgtccgtt caccagaaac agcaacaaga tctggttaat
     6601 gacgcgctga acgtcgaata aataaaggat acacaatgag tagcgtagat attctggtcc
     6661 ctgacctgcc tgaatccgta gccgatgcca ccgtcgcaac ctggcataaa aaacccggcg
     6721 acgcagtcgt acgtgatgaa gtgctggtag aaatcgaaac tgacaaagtg gtactggaag
     6781 taccggcatc agcagacggc attctggatg cggttctgga agatgaaggt acaacggtaa
     6841 cgtctcgtca gatccttggt cgcctgcgtg aaggcaacag cgccggtaaa gaaaccagcg
     6901 ccaaatctga agagaaagcg tccactccgg cgcaacgcca gcaggcatct ctggaagagc
     6961 aaaacaacga tgcgttaagc ccggcgatcc gtcgcctgct ggctgaacac aatctcgacg
     7021 ccagcgccat taaaggcacc ggtgtgggtg gtcgtctgac tcgtgaagat gtggaaaaac
     7081 atctggcgaa agccccggcg aaagagtctg ctccggcagc ggctgctccg gcggcgcaac
     7141 cggctctggc tgcacgtagt gaaaaacgtg tcccgatgac tcgcctgcgt aagcgtgtgg
     7201 cagagcgtct gctggaagcg aaaaactcca ccgccatgct gaccacgttc aacgaagtca
     7261 acatgaagcc gattatggat ctgcgtaagc agtacggtga agcgtttgaa aaacgccacg
     7321 gcatccgtct gggctttatg tccttctacg tgaaagcggt ggttgaagcc ctgaaacgtt
     7381 acccggaagt gaacgcgtct atcgacggcg atgacgtggt ttaccacaac tatttcgacg
     7441 tcagcatggc ggtttctacg ccgcgcggcc tggtgacacc ggtactgcgt gatgtcgata
     7501 ccctcggcat ggcagacatc gagaagaaaa tcaaagagct ggcagtcaaa ggccgtgacg
     7561 gcaagctgac cgttgaagat ctgaccggtg gtaacttcac catcaccaac ggtggtgtgt
     7621 tcggttccct gatgtctacg ccgatcatca acccgccgca gagcgcaatt ctgggtatgc
     7681 acgctatcaa agatcgtccg atggcggtga atggtcaggt tgagatcctg ccgatgatgt
     7741 acctggcgct gtcctacgat caccgtctga tcgatggtcg cgaatccgtg ggcttcctgg
     7801 taacgatcaa agagttgctg gaagatccga cgcgtctgct gctggacgtg tagtagttta
     7861 agcttcacct gcactgtaga ccgacatgga tagggtgtcg gtctacggtt taaaagataa
     7921 cgattactga aggatggaca gaacacatga acttacatga atatcaggca aaacaacttt
     7981 ttgcccgcta tggcttacca gcaccggtgg gttatgcctg tactactccg cgcgaagcag
     8041 aagaagccgc ttcaaaaatc ggtgccggcc cgtgggtagt gaaatgtcag gttcacgctg
     8101 gtggccgcgg taaagcgggc ggtgtgaaag ttgtaaacag caaagaagac atccgtgctt
     8161 ttgcagaaaa ctggctcggc aagcgtctgg taacgtatca aacagatgcc aatggccaac
     8221 cggttaacca gattctggtt gaagcagcga ccgatatcgc taaagagctg tatctcggtg
     8281 ccgttgtaga ccgtagttcc cgtcgtgtgg tctttatggc ctccaccgaa ggcggcgtgg
     8341 aaatcgaaaa agtggcggaa gaaactccgc acctgatcca caaagttgcg cttgatccgc
     8401 tgactggccc gatgccgtat cagggacgcg agctggcgtt caaactgggt ctggaaggta
     8461 aactggttca gcagttcacc aaaatcttca tgggcctggc gaccattttc ctggagcgcg
     8521 acctggcgtt gatcgaaatc aacccgctgg tcatcaccaa acagggcgat ctgatttgcc
     8581 tcgacggcaa actgggcgct gacggcaacg cactgttccg ccagcctgat ctgcgcgaaa
     8641 tgcgtgacca gtcgcaggaa gatccgcgtg aagcacaggc tgcacagtgg gaactgaact
     8701 acgttgcgct ggacggtaac atcggttgta tggttaacgg cgcaggtctg gcgatgggta
     8761 cgatggacat cgttaaactg cacggcggcg aaccggctaa cttccttgac gttggcggcg
     8821 gcgcaaccaa agaacgtgta accgaagcgt tcaaaatcat cctctctgac gacaaagtga
     8881 aagccgttct ggttaacatc ttcggcggta tcgttcgttg cgacctgatc gctgacggta
     8941 tcatcggcgc ggtagcagaa gtgggtgtta acgtaccggt cgtggtacgt ctggaaggta
     9001 acaacgccga actcggcgcg aagaaactgg ctgacagcgg cctgaatatt attgcagcaa
     9061 aaggtctgac ggatgcagct cagcaggttg ttgccgcagt ggaggggaaa taatgtccat
     9121 tttaattgat aaaaacacca aggttatctg ccagggcttt actggtagcc aggggacttt
     9181 ccactcagaa caggccattg catacggcac taaaatggtt ggcggcgtaa ccccaggtaa
     9241 aggcggtact acccacctcg gcctgccggt gttcaacacc gtgcgtgaag ctgttgctgc
     9301 tactggcgct accgcttctg ttatctacgt accagcaccg ttctgcaaag actccattct
     9361 ggaagctatt gatgcaggca tcaaactgat tatcaccatc actgaaggca tcccgacgct
     9421 ggatatgctg actgtgaaag tgaaactgga cgaagccggc gttcgtatga tcggcccgaa
     9481 ctgcccgggc gttatcactc cgggtgaatg caaaatcggt atccagccgg gtcacattca
     9541 caaaccgggt aaagtgggta tcgtttcccg ttccggtaca ctgacctatg aagcggttaa
     9601 acagaccacg gattacggtt tcggtcagtc gacctgcgtt ggtatcggcg gtgacccgat
     9661 ccctggctct aactttatcg acattctcga aatgttcgaa aaagatccgc agaccgaagc
     9721 gatcgtaatg atcggtgaga tcggcggtag cgctgaagaa gaagcggcag cgtacatcaa
     9781 agagcatgtt accaagccag ttgtgggtta catcgctggt gtgactgcgc cgaaaggcaa
     9841 acgtatgggc cacgcgggtg ccatcattgc cggtgggaaa gggactgcgg atgagaaatt
     9901 cgctgctctg gaagccgcag gcgtgaaaac cgttcgcagc ctggcggata tcggtgaagc
     9961 actgaaaact gttctgaaat aaatatctgt aataagaaat agccctcgcc gcttccctct
    10021 acaggaatgg cgaagggctg tcggtttcga catggttggc catcgtatga tggccttttt
    10081 tgtgcttatc gcgatgattt tcgttgcgct atcagggtaa atttatagtc atcggtatta
    10141 aaagcgttgc ggctatattc aaacacccga ccatcaacta aatatccacg cgatactttt
    10201 tcaagaatcg gctttgtctg gctgatatta agcagacggc tcatctcttc ggttggcatc
    10261 agaggaatga tttcctgttc gctacgatcg ataaccattt tcttcacttc ttcaataaag
    10321 tgatattttg aattttccat gacctgccag gtgagatccg ggaacaacgc aagcggcatc
    10381 caggtttctt ccagcgccat tggcttttgc ttgcgatagc gcacgcgctt cacatgccac
    10441 acacgatcct gcgcggtgat ttgtagctgt tgctgaagaa aatcgtcagc cggaatcact
    10501 tcaaatatca ggacttcact gtgtgtatcg acgtgacggt ccgacagttt ttcatcaaaa
    10561 ctggttaact gaaaaatatc gtaattgacc cgctcttctt tgacgtaagt cccgctgccc
    10621 tgaatgcttt cgaggatctg ttgctcgact aactggcgca aagcctgacg caccgtaacc
    10681 cggctgacgc caaactctgt ttgtaatgcc gattcggtag gtaacgcatc gccaggttta
    10741 agctcgccac gcgcaatttg ttcacgaatg cgatcggcaa tctgccggta taagggcttg
    10801 tgtcccattt ttagtatctc attaatacga atttagccat tatgcccgat aaattcatcc
    10861 tgtaaataat acaaatacaa tacaaataat ttcaatcaag tgaaattgat cacataatgg
    10921 tattgtttta tcgggcacac tggcgcgact ataaaaacga tcaagtgagg atcatgatga
    10981 atctgacgac tctgacccac cgcgatgcgt tgtgtctgaa tgcgcgcttt accagccgtg
    11041 aagaggccat ccacgcgttg actcaacgtc ttgctgctct ggggaaaatt tccagtactg
    11101 agcaatttct ggaagaagtg tatcgccgtg aaagccttgg cccgaccgcc ttaggtgaag
    11161 ggttggctgt gccgcatggc aaaactgctg cggtaaaaga agcggcgttt gcggtcgcca
    11221 cactcagcga gccgcttcag tgggaaggcg ttgatggccc ggaagcggtt gatttagtgg
    11281 tgctgctggc gattcccccc aatgaagcgg gtactacgca tatgcaactg ctgacagcgc
    11341 tgaccacgcg cctggcggat gatgagattc gggcgcgtat acagtcggcg acgacgcctg
    11401 atgagttgct ctcggcgctg gatgacaagg gaggcacgca accttctgcc tctttttcca
    11461 acgcgccaac tatcgtctgc gtaacggcct gtccggcggg tattgctcac acctatatgg
    11521 ctgcggaata tctggaaaaa gccggacgca aactcggcgt aaatgtttac gttgaaaaac
    11581 aaggcgctaa cggcattgaa gggcgtttaa cggcggatca actcaatagt gcaaccgcct
    11641 gtatttttgc ggctgaagtc gccatcaagg agagtgagcg ttttaatggc attcccgcgc
    11701 tttcagtgcc tgttgccgag ccgattcgcc atgcagaagc gttgatccaa caagcgctta
    11761 ccctcaagcg tagcgatgag acgcgtaccg tacagcaaga tacgcaaccg gtgaaaagtg
    11821 tcaaaacgga gctgaaacag gcactgttga gcggaatctc ttttgccgta ccgttgattg
    11881 tcgcgggggg cacggtgctg gcggtcgcgg tattactgtc gcaaatcttc gggctacaag
    11941 atctgtttaa tgaagaaaac tcctggctgt ggatgtaccg caagctgggc ggcgggctgc
    12001 tcggaatttt gatggtaccg gtgctcgcgg cctataccgc ctattctctg gcagataaac
    12061 cggcgttagc gccaggcttt gcggctggac ttgccgccaa catgatcggc tccgggtttc
    12121 tcggcgcggt cgttggcgga ttgatagccg gttacttgat gcgctgggtg aaaaatcact
    12181 tgcgtcttgg cagtaaattc aatggcttcc tgacttttta tctctacccg gtgctcggta
    12241 cgttgggagc gggcagtctg atgctgtttg tggtgggaga acctgtcgca tggatcaata
    12301 actcgcttac cgcctggctg aacggtctgt caggaagtaa cgcgctgttg ctgggtgcca
    12361 ttctcggttt tatgtgttcc tttgaccttg gagggccagt gaataaagcc gcttatgcat
    12421 tctgcctggg cgcaatggcg aacggcgttt acggcccgta tgccattttc gcctccgtca
    12481 aaatggtttc ggcatttacc gtaaccgctt ccacgatgct cgcaccgcgc ctgtttaaag
    12541 agtttgaaat tgagaccggg aaatccacct ggctgttagg gctggcaggt attaccgaag
    12601 gggcgatccc gatggcgatt gaagatccgc tgcgggttat tggttcgttt gtgctgggct
    12661 ctatggtaac gggcgctatt gtcggtgcga tgaatatcgg cctttcgaca cccggtgccg
    12721 gcattttctc gctcttttta cttcatgata atggcgcggg cggtgttatg gcggcaattg
    12781 gctggtttgg tgcggcattg gtgggggctg caatctcgac tgcaattctc ctgatgtggc
    12841 ggcgtcacgc ggttaagcat ggcaactatc tgactgatgg cgtaatgcca taaacaaaaa
    12901 ggaaacgacg atgaaagcgg tatctcgcgt tcacatcacc ccgcatatgc actgggatcg
    12961 ggagtggtat ttcaccaccg aagagtcacg cattctgctg gtcaataata tggaagagat
    13021 cctgtgccga ctggaacagg acaacgaata caaatattac gtactcgacg ggcaaacggc
    13081 gatcctcgaa gattatttcg cggtgaaacc ggaaaacaaa gaccgtgtga agaaacaggt
    13141 agaagccggc aagttgatta tcggcccctg gtatacccag accgatacca cgattgtttc
    13201 tgcggaatcc atcgtccgta atctgatgta cggaatgcgt gactgcctcg cgtttggcga
    13261 gccgatgaaa ataggttatt taccggattc ctttggcatg tccgggcagc ttccgcatat
    13321 ctacaatgga tttggcatta cccgcaccat gttctggcgt ggatgttctg agcgccacgg
    13381 tactgataaa accgagtttt tgtggcaaag cagtgacggt agcgaagtga cggcgcaagt
    13441 gttgccgctg ggctacgcca tcggtaagta cttacctgcc gacgaagacg gattacgcaa
    13501 gcgcctcgac agttattttg atgtgctgga aaaagcgtca gtaaccaaag aaattttatt
    13561 gccgaatggt catgaccaga tgccattgca gcaaaatatc ttcgaagtga tggataagct
    13621 acgtgagatc taccctcaac gtaagtttgt gatgagccgc tttgaagagg tatttgagaa
    13681 gatcgaagcg cagcgagata atctggcaac cctgaaaggg gaatttattg atggcaaata
    13741 tatgcgcgtg catcgcacca tcggttctac gcgtatggat atcaaaattg cccacgcgcg
    13801 tattgaaaat aagattgtta atctgctgga accgctggca acactggcct ggacgttggg
    13861 ttttgaatac caccacggct tgctggagaa aatgtggaaa gagatcttaa aaaatcatgc
    13921 ccacgacagt atcggttgtt gttgcagtga caaagttcat cgcgaaatcg tcgcccgctt
    13981 cgaactggct gaagacatgg cggataatct gattcgtttc tacatgcgca aaattgccga
    14041 caacatgccg cagagcgacg ccgacaaact cgtcctgttt aacctgatgc cctggccgcg
    14101 tgaagaagtg atcaacacca ctgtgcgcct gcgcgccagc cagtttaatt tgcgggacga
    14161 tcgcggccag cctgtgccgt attttattcg ccatgcccgt gagatcgatc caggcctaat
    14221 cgatcggcaa attgttcatt atggcaatta tgatcccttt atggagttcg acatccagat
    14281 caaccagatt gtcccttcta tgggctatcg cacgctttat attgaagcga atcagcctgg
    14341 caacgtgatt gcggcaaaaa gtgacgctga agggattctg gaaaatgctt tctggcagat
    14401 tgcgctcaat gaggatggtt ctctgcaact ggtagataaa gacagcggtg tgcgctatga
    14461 ccgggtattg caaattgaag aaagctctga tgatggtgat gaatatgact attcacccgc
    14521 aaaagaagag tgggtaatta ccgcggcgaa cgcgaaaccg caatgcgata ttattcatga
    14581 agcctggcag agcagggctg ttatccgtta tgaaatggcg gtgccgcgta atctgcaaga
    14641 acgcagcgca agacaatcca ctggcagagt aggggtggag atggttgtca ctctgagtca
    14701 taacagcaga cgtattgatg tggatatcaa ccttgataac caggctgacg atcatcgcct
    14761 tcgcgtcctg atccctacac cttttaacac cgacagtgtt ctggcagata cgcagtttgg
    14821 ttcgctaacg cgccccgtga acgacagtgc aatgaacaac tggcagcaag aaggctggaa
    14881 agaagcgccg gttccggtat ggaatatgct caactatgtt gccttacagg aagggcgtaa
    14941 cggcatggct gtctttagcg aagggctacg tgaatttgaa gtcatcggtg aagagaagaa
    15001 aacctttgcc attacgttgc tgcgtggcgt gggcttactg ggcaaagaag atctgctttt
    15061 aaggcctggg cggccttcgg gaattaaaat gccagtcccg gactcacaac tacgtggtct
    15121 gctttcttgt cgcctaagtt tattgagtta taccggtacg ccaaccgccg ctggtgtagc
    15181 tcagcaggcg cgagcatggc tgactccagt acagtgttac aacaaaatcc catgggatgc
    15241 gatgaagctc aacaaagccg gattcaacgt gccggaaagt tatagtttgt tgaaaatgcc
    15301 cccagtggga tgcctgataa gcgcacttaa gaaagctgaa gaccgacaag aagtgatttt
    15361 acggctgttt aatccggctg aatcagcaac ctgtgatgcg actgttgctt tcagtcgcga
    15421 ggtgatttct tgctcagaaa cgatgatgga tgaacacatt accaccgagg aaaatcaagg
    15481 ttcaaatcta tcggggcctt ttttacccgg ccagtcacgg acgttcagtt accggcttgc
    15541 ctgaatagca atcaaaccga agccacatat gcgcggccag attgttgaca aagggcgctt
    15601 tgttcatgcc ggatacggca tgaacgcttt attcggtcta caaaagcagg caaattcaat
    15661 atattgcaga gatgatgtag acactggcaa gcgtagcgca tcaggtaatt ttgcgtttat
    15721 cttcactctc aagccacgta tatgtggctt tatttttaac aaaataataa ctctgggtga
    15781 gttaattata
//
LOCUS       DAZGJW010000107        15615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZGJW010000107 DAZGJW010000000
VERSION     DAZGJW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.107"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..113)
                     /locus_tag="ACHKIK_003451"
     CDS             complement(<1..113)
                     /locus_tag="ACHKIK_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFO9084561.1"
                     /translation="MLSYLMAIHFVLFGDSTNLKNFWKYEVIRRKRMDIWRL"
     gene            complement(270..758)
                     /locus_tag="ACHKIK_003452"
     CDS             complement(270..758)
                     /locus_tag="ACHKIK_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9084562.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSATSSVKG
                     LL"
     gene            complement(771..3578)
                     /locus_tag="ACHKIK_003453"
     CDS             complement(771..3578)
                     /locus_tag="ACHKIK_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9084563.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RAREWYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVSVSDA
                     GGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAVGDGSLPKLR
                     KELEGARGEAERTAKVMANNLDGDLKSLGSAWEGLRIRIADLIDGPLRSVTQWLTRVV
                     SRVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLNPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPAASRNREPAVSRFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            complement(3565..3720)
                     /locus_tag="ACHKIK_003454"
     CDS             complement(3565..3720)
                     /locus_tag="ACHKIK_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO9084564.1"
                     /translation="MCFEHIEDFVADIAVIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            complement(3729..4094)
                     /locus_tag="ACHKIK_003455"
     CDS             complement(3729..4094)
                     /locus_tag="ACHKIK_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9084565.1"
                     /translation="MKETKNIDTENTVVADTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGGKNEQATEA"
     gene            complement(4149..4661)
                     /locus_tag="ACHKIK_003456"
     CDS             complement(4149..4661)
                     /locus_tag="ACHKIK_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO9084566.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            complement(4661..5845)
                     /locus_tag="ACHKIK_003457"
     CDS             complement(4661..5845)
                     /locus_tag="ACHKIK_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO9084567.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEGGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCHDCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGVRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNSTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEYFEPAFAVLG
                     GA"
     gene            6003..7103
                     /locus_tag="ACHKIK_003458"
     CDS             6003..7103
                     /locus_tag="ACHKIK_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO9084568.1"
                     /translation="MTDTTMQLLSQSTDPVKMPDFDILAEGETLSGVAERLMSLSLTD
                     NRGFEADLLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSSNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPKKEKSSSREGDYMEGAEGNVFVLR
                     KTYQNEQAARRAAAAKWQQLQRGAASFSITLARGRAELYPEMHGTVTGFKSDIDNQDW
                     IIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            7503..8642
                     /locus_tag="ACHKIK_003459"
     CDS             7503..8642
                     /locus_tag="ACHKIK_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014606071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HFO9084569.1"
                     /translation="MGNNKVKLGRNDKCWCNSGLKYKKCHLGRDRETPVSKAEALKFS
                     KSLSQRKACYAPASMHEDCDKRIVQAHTISKSSSLKAIADSSNHVMGVIMNLASLMRN
                     NGKWLPEKIGINQASTFTGFCAVHDRILFSCLENEKFTGTDEQCFALMFRSLSKEIYA
                     KEGGVLSSDFAKNADKGKLQVEQYYIQKLIDQHQSGLNAAIIELNNLKTSLDRILLSR
                     QFSEIESVIFTFAEPLPIVVSSILSPERDFDGTKIQDLSDLTVSAEQVCFNAFSCGGK
                     GYVVFSWLRTSTIIRRFIRTLIKIPANRMFNTLLYFFFTKAENTYSSPEWWDRLSDKQ
                     RENIGNMIMSGVEFFGRPTSQVDHSVDYNAAALEEITCSNNEICS"
     gene            8932..9192
                     /locus_tag="ACHKIK_003460"
     CDS             8932..9192
                     /locus_tag="ACHKIK_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO9084570.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEGIPWHELPASHRGNNQMSLPLPQN"
     gene            9383..9523
                     /locus_tag="ACHKIK_003461"
     CDS             9383..9523
                     /locus_tag="ACHKIK_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9084571.1"
                     /translation="MSQKSLTAITFCVTVILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(9712..9993)
                     /locus_tag="ACHKIK_003462"
     CDS             complement(9712..9993)
                     /locus_tag="ACHKIK_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084572.1"
                     /translation="MRCGLLMAICLCLVSFSGYAATGKSDAQIKKEIIKESIESYPGN
                     CACPYNHARNGSRCGGRSAYSRNGGYDVVCYEGDVTDEMIRQWKQENAE"
     gene            complement(10532..10618)
                     /locus_tag="ACHKIK_003463"
     tRNA            complement(10532..10618)
                     /locus_tag="ACHKIK_003463"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10582..10584),aa:Leu,seq:taa)
     gene            complement(10631..10704)
                     /locus_tag="ACHKIK_003464"
     tRNA            complement(10631..10704)
                     /locus_tag="ACHKIK_003464"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10670..10672),aa:Cys,seq:gca)
     gene            complement(10759..10834)
                     /locus_tag="ACHKIK_003465"
     tRNA            complement(10759..10834)
                     /locus_tag="ACHKIK_003465"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10799..10801),aa:Gly,seq:gcc)
     gene            complement(10986..11534)
                     /gene="pgsA"
                     /locus_tag="ACHKIK_003466"
     CDS             complement(10986..11534)
                     /gene="pgsA"
                     /locus_tag="ACHKIK_003466"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO9084573.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11591..13423)
                     /gene="uvrC"
                     /locus_tag="ACHKIK_003467"
     CDS             complement(11591..13423)
                     /gene="uvrC"
                     /locus_tag="ACHKIK_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO9084574.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGVDRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13420..14076)
                     /gene="uvrY"
                     /locus_tag="ACHKIK_003468"
     CDS             complement(13420..14076)
                     /gene="uvrY"
                     /locus_tag="ACHKIK_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO9084575.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14218..14334
                     /locus_tag="ACHKIK_003469"
     CDS             14218..14334
                     /locus_tag="ACHKIK_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9084576.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
     gene            14372..14548
                     /gene="yecU"
                     /locus_tag="ACHKIK_003470"
     CDS             14372..14548
                     /gene="yecU"
                     /locus_tag="ACHKIK_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO9084577.1"
                     /translation="MIKIFIGHYINAFNSTVDDTLKKQPLLFLAKLMVYSAALTLFTA
                     NFHCNMTRKINEYA"
     gene            14535..14759
                     /gene="yecF"
                     /locus_tag="ACHKIK_003471"
     CDS             14535..14759
                     /gene="yecF"
                     /locus_tag="ACHKIK_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO9084578.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14827..15549)
                     /gene="sdiA"
                     /locus_tag="ACHKIK_003472"
     CDS             complement(14827..15549)
                     /gene="sdiA"
                     /locus_tag="ACHKIK_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO9084579.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         4014 a         4143 c         3673 g         3785 t
ORIGIN      
        1 agcctccaga tatccatacg tttccgccga attacttcat atttccagaa gttttttaaa
       61 ttagtagagt ccccaaataa aacaaagtga attgccatta agtaagacag cacgataatc
      121 tccttaatta ttatttcaga ccacacatat tataaggtta agagattata aaatcctgtt
      181 gtttgttatt caaaaacaat tttctgagaa ggacatacaa cagcaagtcg ccagtcacct
      241 tcatcaggaa attggcgaca tacgttaaat cagagcagcc ccttaactga gctggtcgcg
      301 ctattaaggg atgatgtcac cttatctttg aagccggaca acatatcgct gaacgatgag
      361 gattgcaggc gctcccgcaa atcctcatca cagcgttcaa gagtcagtga aaattctatc
      421 tttttcgcct taccgtagcg atcaaactcg gagcgggtcg tattcgtttc ggtcaggaca
      481 tacatgccgt aaatctgccc gacgccatca atcagaggcc agggccgtcc tgtatacgcc
      541 tgcgtggtca gcagagacag cgacacttcg ccacctgtaa tttcaggata aagcacacca
      601 gaaagaacga tgcgatcatc acctgcaccg atatactgcc agcttgctga acggttaacg
      661 cgttcatttt tcacatgccg ccagcttttg ttttgctgta actgctgatg cggcagcgtg
      721 cgcagctcaa aaacaaacat gccatagatc atcatcatgg ccatgactcc tcaatcttta
      781 tcgtaaaaac tgccacgccc ggcacgggcg cgccgttcca tttctgccct gaccatttca
      841 ccgaccagtt tcgccagttc gcggggattc tgcgtaacaa cgttatgcag atgaacatga
      901 atttcaccgc caaatctgga gacagcaggc tcccgattac gggaagctgc aggaactgat
      961 gccactggcg atcgtatggc ctccgccacc gggcgggagc tggccgcaac aacagggacc
     1021 agcgccggag gcagcggagc cgggactacg ggtgtgatat taattgcggg ggcaggctta
     1081 ctgacctgcg caatcttccg ctcctgccac tccccacgaa cagcaagtgc gcggggcagg
     1141 ttcttaaaga caatatcgcc agggccaatg cgttttttcg tctcatcaac cagcttacct
     1201 gtgttatcag caattttgct gagtctgcgt agcgtcccgg tattgctgtc tgtgagcggt
     1261 ttgttgtctt tgggtttatc acctccggtg ccattgccat tttccacagg cttcggcgga
     1321 ttgattttcg ccaggtcccc ctgaagtaag gcaaccttat cctgaagaat ggccgcacgc
     1381 tgtgcgtctt cgattttctt gcgcgccctt tccgcttcat ccggaaggac gccaagtttt
     1441 tcaagtatcc acgccagcgt atccagtagc atttttgcag gtgtcagaac aagttgtaac
     1501 gcaccgccaa gaacgttacc gaatatctcg ccagcactgg tacatttatc cagcgtttcc
     1561 ttgctggact ccatcggtga cagcagcgat ttaaaccagt taaacacctg gctgatcccg
     1621 cttccgattg cgtcaaaaac agggccaaac cgttcaaagg tttcacgcaa cgggttcagc
     1681 ctttccataa tcccgctgaa caccccggca aaaaatgccc tgatgggatc ccagtatttc
     1741 cagataagga cggcagctcc ggcaagcgca gccacgataa gaccaaccgg actgaacagc
     1801 gccccgatag cgcctcccag taaagaaacg gaacccgtca ccattcccca tagtgctggc
     1861 aggaccctga cagcattcat tgatccggtc aggagagaaa aaccaagacg cagttttgcc
     1921 agcggaccag caagcacacc aatagccagc gacaacgagc caaccgttgc agtcattgcc
     1981 agcaacgcac cgcctgctat cagtagctgg cgcgtcagtg ccggatgggc ctgcgccagc
     2041 gccgtcaccc ttgataccac ccgcgtgagc cactgcgtga cagaacgcag cggaccgtca
     2101 atcagatctg caatgcggat gcgcaaccct tcccatgcac tgccgagtga tttcagatcg
     2161 ccgtcaaggt tgttggccat aacctttgct gtgcgttcag cctcaccgcg cgcgccttca
     2221 agttcttttc tcagtttggg taaggaaccg tcacccactg catcaacgag cgccataaac
     2281 gatgtgaaag cctcttctcc ggcaatgtcc ttaaagaacg atacccggtc aacttccccg
     2341 tatttgcggg tggctttata aaggtcggcc agcacatcct ccatcgggcg cattttaccc
     2401 ccggcatccg agacggacac gcccagctct ttcagagctt ctgccgccgc ctttggcggt
     2461 gatgccagac gagccaggct ggcacgcatt gccgtcccgg catcactccc tctgatgccc
     2521 atattcgcca gcacaccagc catcgctgcg gcctgctcca gcgatattcc cagcttaccc
     2581 gccaccggac ctgcatattt catggtttcg cccagtgcgc gaaggtcagt gttggtacgg
     2641 gtaaacgctg cggtgagtgt gtcaccgacc cggtccatct ggtcagcaga aaggccgaac
     2701 tgcgtcagga tatttgagcc aatatctgcc gtctcgccga gatccatacc gccagccgtt
     2761 gccatgctca gcactccggg aagcgcagcc tgaatggcct gcggtgtgaa gcctgccatt
     2821 gcaagaaatg cctgcccact ggcggcatcg cctgcggtga actgcgtttc agagccaagt
     2881 tttaacgcct gctcacgcag cgccttaaac tgcgggctgt tctggtcgat tcgcgtcagc
     2941 gcctgaacgc gggacatctc tttgccgaac ccgatcgcgg gctgcaaaaa acgcccggca
     3001 gcatagccgc ccgccgctgc cgcaccaatt gccagcgcac cacctgtttt cagttttccc
     3061 gcggtttcct gcgcgcgcga ataccactca cgcgcccgcg ttacacgcgc aagcgcctgc
     3121 cgttcgcgtt caagctggtt gttgtactgt tcggtgcgtc tgatggcctg ctggatggtg
     3181 ttatcgctgc ctgtcaggga aatgccgtgg cgtttcagct ctccgccaag ctcccgcatt
     3241 ttctgaattt cccgtgtgcg cgattcattc aggcgttcaa gccgggtgct taactgctgc
     3301 atcagctttt gttgtttttc gctgagcact gtacccgtgc gttgtaactg attaagggcg
     3361 ttaagctggc gtcgtgcttt cacgataccc gcatccgctt tactgacagc gtcgcgggcg
     3421 cgctcaaatg aacgagcctg acgctcgaga tttttaatcg ccccctgcgt tcgctggatg
     3481 gagtcaccaa actgccccat caggcggcgg gcgttttcgg caggccgggt cagcctgtca
     3541 acggcgctga aagcgacccg gatatcaaga gtcttcattg tctgcattcc cgctgcgaag
     3601 tgccgcccgc tcacgccagc taaccacttc gccgggcgtc atcatgaaga tttcggcggg
     3661 cgaccagtta aaaataacgg caatatctgc cacaaagtct tctatgtgct caaagcacac
     3721 aaccgtgatc aggcttccgt cgcctgttcg ttcttcccgc cagagtccgc accgctcaaa
     3781 aaatttacgg caaccacaca taactgaata aagtcacggg atgccatttt tttgatcgtc
     3841 acttcatcca gtcgcggtga tgtcacgcgt gacagcagcg taaacatgga ttccgctttc
     3901 agattcagca catcagacag cgacaaatct cgcagagatc cagcctgctc aatagccccg
     3961 gtgatctcca catacgtgat tttttcgccg cctcgctcaa ttggttgggt aagttttaca
     4021 ccacgctcac tggtttcttt cacagtgtca gcaactaccg tgttttcggt atcgatgttt
     4081 ttcgtctctt tcatcaggaa actcctttca gtcagaggcg acgcactgcg ccgcctgcat
     4141 attacttatc agccaagccc gagcgcggaa cggatgcgat cgggcacaat gtccttgccg
     4201 tccttccggt aaatgaagtt cagcaggtca atctcccaca acgggcgatc gttaacactc
     4261 agcttgtagt aggtgttttt aatggcgtaa gtgtgtgatg tggcttcgcc ctgtttggct
     4321 tcccccatat caatttccgt cacacgtccg cgcatttcga cttcatacag gtcgctttct
     4381 gcatcggtgt aatattcacc cgcaaaacgc agcagcgtgc cgtcaatcgt gccgccatac
     4441 ttaaggaaca gctcacgaac tgcgcccccc atgacaaagc tcgcatcaag cgcggagtcg
     4501 tccagaccga gatcaatact taccgcaccc atcatgccac caccccggta gctgtcggtt
     4561 ttgcgcgtca gcttaggcag agtgacggac gtcaccttac ccacttcgtt ttcaccatcc
     4621 acaaacagcg taaaaaagcg aagatgtttt ggtacagcca tcaggcacct cccagcaccg
     4681 caaatgcggg ttcaaagtat tcatcagtaa acgtctggta aagctccatg tcttccagtg
     4741 gcggaacggg cgtatatttg tagcgaatac gcacacgccc ctgacgtaaa tccgtggtgc
     4801 tgttatccac cacgtcatac cagcactccg cgccaatcag tttcccggca gtaaccagcg
     4861 aatccagttt tgccctgatg gcactgataa catccttcac gttcgcaggc gtcagtggac
     4921 tgtcgatggt ttcaaactgc gcttccgcaa ttgaatcagc cagcacctgt gcggttcggg
     4981 tatacacctc aaagatgtag gcgttcgttt ccggtgtgcg gttgccccag aagcggaccc
     5041 cgttgcgacg aataatggtc gtgatttctt tgttgttgag gctgttggca tcactgtctt
     5101 cggcctgcaa cgaccagaaa acatgcctgg acatccccag cacattttta acaggaacgt
     5161 tggacagcga tttgtgccag ccctgctcat ggtcaatgta cgcacgaagg ccgcacgcat
     5221 aggcaggcgc ggggaacgtt tcgtttttgc cacttttcgg gttgtaggcg atgaagtccg
     5281 gccataagag catcacctca cgttcgttga atttctggcg gtaggtaatt gcctcagcca
     5341 tcgtgttaca gtcatgacat gaggcataca caaacgcgcg cagtttaccc gcaatcacgc
     5401 acagggattt tgtcaccgcc tccgtgtcca gctccggcgc ggccagaata cgcggacggt
     5461 atccgatgct ttcatcctgc tctgcaacaa gcagcgcata catccccgta tagctgccgt
     5521 cagattcaga accaccgata accagttgat cctgcgtctt accgccttct tctttgtgtt
     5581 cagccacgcg aacgacgatc acttttgtgc tcacctggtc tgcaatagcc ttaagcgcac
     5641 gataaagcgt ccccgtagtc ccgcattttc ccagcacgtc attgacgcgg gtcagcagtg
     5701 tgggcttgtt cagcgggaac agctccgcgt ccgcatcatc cgccgttgcc acgataccga
     5761 taacactgga atcaacatca ttaatcgctg ttaccaggtc ggtactttcc gtaacacggg
     5821 caccatgaaa acgagtttca ctcatagctt cagccccttg tatccgttaa atgattcggc
     5881 aacaatcatc acccaccacg cgcgtaatct cacccctgcg ccgttctccc gccacggcga
     5941 caacaaaaag cagtaacccc ctatgcacgc acatgcgacc atgccgcaca gggagggaac
     6001 agatgaccga taccaccatg caattgctca gtcagagcac agaccccgtg aaaatgccgg
     6061 attttgatat tctcgcggag ggggaaacac tgtccggcgt ggcagagcgc ctgatgagcc
     6121 tgtcactgac cgacaaccgg ggatttgaag cggacctgct caccatcacg ctggatgatg
     6181 cggatggtca gttgcagcta ccgccacggg gcgcgcgcct gacggttctc attggctgga
     6241 aaggagaacc gctgacagaa aaaggcactt acattgttga tgaaatcgct cacgaaggac
     6301 cgccggacag gctgactgtt tcagccagaa gcgcagattt tcgggatgaa tttaacgtta
     6361 aacgtgaggt gtcctggcat gatgtgaccg ttgagcgcgt ggtatccgcc atcgctcatc
     6421 ggtacggtct gaaaccacaa atcagcgaaa tgctgatgga tattgaaatc gaccacgccg
     6481 accagaccga agaaagcgac atgtccttcc ttacgcgcat ggcggaaatg ctgggcgcaa
     6541 tcactacggt aaaaagcagt aatctgttat tcatcatgcc aggcggtggc gtgaacgcac
     6601 agggccagcc gttgccatcg ttcgccatca cgcgcagcag tggcgatcgc catcagttcc
     6661 gcattgctga ccgcgaagcg tatacggggg tacgtgctta ctggcttgat cttaattacg
     6721 ggaaaaagaa aaaagtcagc gtgaaacgcc gcaaaccgaa aaaggagaaa agcagcagcc
     6781 gtgaaggtga ctatatggaa ggtgcggaag gcaacgtgtt tgtgttacgc aagacttatc
     6841 agaacgagca ggcagcaaga cgtgcagcgg cggcaaagtg gcagcagcta caacgcggag
     6901 ccgcatcatt ctccatcacg ctggcgcgtg gacgcgcaga actctatccc gaaatgcatg
     6961 gcacggtgac aggcttcaaa agtgacatcg ataatcagga ctggattatt gcaaaagccg
     7021 agcacaccat tgataacagc ggctttacca cacagcttga gcttgaagca aaaatcccgg
     7081 aatggatagc agaaacagaa tgagcaaatt agatgtattg actcaaacca gaactgacac
     7141 actcacagca cagtgccaga cccaatctgg cagcccgctt tgtgcctaga gcaggtgttg
     7201 cttatgtaac agttaatcca cggatagtgg tcatgaatgc tggttgaagt tgtattgcca
     7261 tgagtggtca attagggact cacttattat cgcgtgctca ccattgatgg gtaagatcat
     7321 gcatttcggc tactgtactt cgcgaatcct ccgcacacag gcaacacctg atttaagaac
     7381 agagggagat gatgacgcaa ggaagattta ttatcgtgca tgtggcagat gatcttttag
     7441 ctgccggagt gataaattac acctattttc gataagtgtt tttgttatag aacattaatt
     7501 tcgtgggaaa caataaagtg aaattaggaa gaaatgataa gtgctggtgt aattcaggac
     7561 ttaagtataa aaagtgccat cttggtcgag atcgtgaaac tccggtatcc aaagccgagg
     7621 cactgaaatt ttcaaagtct ctctcgcaga gaaaggcatg ttatgcgcca gcatccatgc
     7681 acgaagattg tgataaacgc atcgttcagg cgcatacgat atctaagagc agtagcctta
     7741 aggcgatcgc cgactccagc aatcacgtca tgggggtgat aatgaactta gcaagcctga
     7801 tgagaaacaa cggaaaatgg cttcccgaga agatcggtat caatcaggca tccactttta
     7861 caggattttg tgcagttcat gacagaatct tattttcatg cctagagaac gaaaaattta
     7921 caggcacgga cgagcagtgc tttgccttaa tgtttcgttc attgtctaaa gaaatttatg
     7981 ccaaagaagg gggagtgcta tcttctgatt ttgctaaaaa tgccgataaa ggaaagctac
     8041 aagttgaaca gtattatatt cagaaattaa tcgaccagca ccaaagtggc ttaaatgcag
     8101 caataattga attaaataat cttaaaacta gcctcgaccg catcctactt agcaggcaat
     8161 tttcagaaat tgaaagtgtt atcttcactt ttgcagagcc tttaccaatt gtagtttcgt
     8221 caattctttc accggagagg gatttcgatg ggaccaaaat tcaggatttg agcgatctta
     8281 cagtgtcggc ggaacaagta tgtttcaatg ctttttcctg cggaggtaag ggatatgtag
     8341 tgttcagttg gctgagaact tcaacaatta tcagacgttt tatacggaca ctaattaaga
     8401 tcccagctaa caggatgttc aatacccttc tgtatttttt cttcacaaag gccgaaaata
     8461 catattcatc ccctgagtgg tgggacaggc tcagtgataa acagcgagag aatatcggca
     8521 acatgattat gtcaggggtt gaattttttg gcagacctac atcacaggtt gatcattccg
     8581 ttgattataa cgccgctgca cttgaagaga taacatgcag caacaacgaa atttgcagct
     8641 gaatgtttac atgctgttag aaaaagcggg ctgaacaatc atttcgggat tgtaacagat
     8701 aagatggcac tgcccgcttg cttcccgata tgcacctcag ccaataaact tatgtaagca
     8761 accgatttcc atttatgcgg aggttatcaa agttgttgga gttgcgaact tccgctcttc
     8821 gcacaaagcg taccgctagg tttgattgta ttctggctgc gcaaactgtc agttggagtc
     8881 tgagctatta caacttagaa tagcggcagc accacgttaa gggaggtcgc tatgttccgt
     8941 tgtccgcttt gtggcgcatc tgcccgaatc cgcaccagtc gtccggaaaa cgattcaaac
     9001 actgtgcggc aaaagtatta ccagtgtaac aacctggaat gcggcgtatg cttctcaaca
     9061 ctggaagctt tccataaatt cacatcaaaa cacgcctccg gcgttcactc ttcggaaggt
     9121 atcccgtggc atgagctgcc agcttcacac aggggaaaca atcagatgag tttgccttta
     9181 cctcagaatt aacaggcaga attgccggag taacaaaaaa acgatagatt acccgcgggt
     9241 gcctttcggc tgatggtcgg aggaataccc gaaggccgga tgtggaaagg ccccggaaaa
     9301 catttctgtt taaccgaggc cctaacatat ctaccttaag caagtgatag gttagcgcct
     9361 ctccaacaaa ggagcaagcg ctatgtcgca aaaatcgctt acagccatca cgttctgcgt
     9421 gacggtaatc ctcatcatct ggatgctaca cggttcgctg tgtgaaatac ggatgagctt
     9481 ctggggagcg gagtttgcgg cgttcttaca gtgtaagcag taaggaaacc gcgacggggg
     9541 agcaatcccc cgtcaatcgg ttgctaaggt tggctgaagt ggcaccctat ctcacagaca
     9601 tgaacaacaa acccgcagcg taagaactgc gggttttctt tttggaacaa tcactagcta
     9661 gtgtaccgtt aacggcacat aatgctcgca caacaaacct gttaaaagag attactcagc
     9721 attctcctgc ttccattgcc ggatcatttc atcggtaaca tcaccctcat aacataccac
     9781 gtcataccct ccattacggc tatatgcact gcgtccacca catctactac cattccttgc
     9841 atgattataa ggacacgcac aattgcctgg ataagattca atggattctt taattatttc
     9901 ttttttgatc tgagcatcag actttcctgt tgctgcatat ccactaaaag atactaaaca
     9961 aaggcagatt gccattaata aaccacatcg cataccgata cactctatta ctaaccaatc
    10021 atagcccaca tcataaaatc gagatgagga gtacaatacc tttaacaaga aggtgtcata
    10081 acgataatat gtcgtgcaat tcatcaaaaa aaagatctat atcaaagaga tagaactctt
    10141 atcagtgctt cagacatcgg cattcgaggc agcagaaaaa cagtaaatct gacgaatttg
    10201 ccttttccca agatgttgtc agattaaaaa gatagattag cgcaggtacc tgttggtgga
    10261 ttgcaggagg aaaaatgaag tcgagtgaaa aaatcccaga aaaatttttg tttaacagag
    10321 gtcctggttc actatgcgaa atacggagga gctgcagaag aattctgctt catccttaga
    10381 gtttaaacag cacgtgcact accattgttt ctgcatatcg ccacaccatc gccactctac
    10441 cgccattgca caaaatacaa atacgaaaaa accacccgaa ggtggtttca cgacactgct
    10501 tattgctttg attttattct tatctttccc atggtacccg gagcgggact tgaacccgca
    10561 cagcgcgaac gccgagggat tttaaatccc ttgtgtctac cgattccacc atccgggctc
    10621 gggaagaaag tggaggcgcg ttccggagtc gaaccggact agacggattt gcaatccgct
    10681 acataaccgc tttgttaacg cgccaaattc ttcaggcctt tcagccagac atccgcttga
    10741 cgccgatgtc ttttaaactg gagcgggaaa cgagactcga actcgcgacc ccgaccttgg
    10801 caaggtcgtg ctctaccaac tgagctattc ccgcattcat caagcaatca gttaatcact
    10861 tgattttatt atcgtctggc aatcagtgcc gccgttcgat gcgttgcatt ctacttacct
    10921 ggcgcgatga gtcaacgata tttttcacca cttttgatcg tttgctgaaa attacgccga
    10981 aacgatcact gatcaagcaa atctgcacgc gcagcgctca aatattgcaa cattgaccac
    11041 agagtcagta ccgcagccac aaagaaaagt gcaataccgg cgtactcaac ccaaatgttc
    11101 ggacgccaca gcagccaggc caacgccacc atctgggcag tggttttcac tttcccaatc
    11161 caggagacag ccacgctact gcgtttaccc aactccgcca tccattcgcg tagcgcagaa
    11221 ataataattt cacgggcgat catcgttgcc gccggtaggg tcacccacca gctgtgataa
    11281 tgctcggtta ccagcaccat ggcgatagcc acgagaactt tatctgctac agggtcaagg
    11341 aaagcaccaa accgggtact ctggttccag cggcgtgcca gaaaaccatc gaaccagtca
    11401 gtcaccgccg cgacgcagaa aatgagcgcg gcggcaaacg gcgaccaggt gacaggcaga
    11461 taaaagacca atacaaagaa tgggataagg atgacacgga acagtgtaag caacgtaggg
    11521 atattaaatt gcataatgac gggtaactat ctgttgtcag taagattacc cctatgttgc
    11581 tacagagaca tcaatgtttc aacgaccaga agatcttttc tgccagacct tgcgaaatac
    11641 ccggcacttt tgcaatttcc tcgacgctgg cgttacgtaa accttgcaaa ccgcccatat
    11701 atttcaacaa catttgccga cgttttggcc cgacgccttc aatggtttcc agggagctgg
    11761 tatttttgac cttcgcccgt tttttacggt gcccgccaat cgcgtgatcg tgtgattcat
    11821 cgcgaatatg ctggataaca tgcagcgcgg gagaatctgg cggcaaactg aatccctcac
    11881 cttccggctc aaagaacagt gtttccagcc cagccttacg atctactcct ttggcaacgc
    11941 caagtagcag tggatgattt ttatcccatg agacatccag ttcggcgaag acatttttcg
    12001 cctgcgcaag ctggcctttg ccgccgtcga taaggatcac atccgggatc ttactgtcgt
    12061 caatggcttt accataacgc cgacgcagca cctgattcat cgccgcataa tcatcgcccg
    12121 gcgtgatgcc tgtaatgtta tagcgccgat actccgcacg cagcgggccg ttagcatcaa
    12181 acaccacaca ggaagcgacg gtttgttcgc ccatggtatg gctgatgtca aagcactcca
    12241 tccgcttcac ttccggcaat ttcaacacgc tggcaagcgc ggtcagtcgc tggtgaacgg
    12301 tagattgctg cgaaagtttg ctggttaagg ccgtcgccgc attggtgcgc gcgagtttca
    12361 gataacgcgc cctatcgccg cgaggtttgg tttgaacatt aatcttgcgt cccgccagtt
    12421 ctgaaaggga atcggcgagc agcgttttat cgctaagatt aaaatcgagc aggatctcac
    12481 ccggtaaggt gcgcatctgg ctgccttgta aatagaactg gcctacgaag gtttctacca
    12541 cctcgctcag ttccgtaccg ccaggcactt tcgggaaata gctgcggctg ccgagcactt
    12601 tgccctgacg aatgaacaat acgtggacac aagccatgcc cgcatcgaac gccacaccaa
    12661 taacgtcgag gtcgtcgccg gtattggaaa cgaattgttt ttcggtgacg cgtcgcaccg
    12721 cctgaatttg gtcgcgaata cgtgcagctt cttcaaactc cagattctgg ctggcagttt
    12781 ccatacgact aatgagttgc gtaagcacct gatcatcttt gccagacaaa aacaggcgca
    12841 catactcgac ctgctgagcg tattcttctt cactcaccag tccttcaacg cacggtccca
    12901 gacagcgccc tatctggtat tgcagacacg gacgcgagcg attgcgataa acactatttt
    12961 cgcactggcg aatggggaaa atcttttgca gtagcgccag tgtttcacgt acggcatagc
    13021 cattcgggaa cgggccgaaa tattcacctt tggcatgctt cgcaccacga tgcatcgcca
    13081 gacgcgggtg ggtatcgcca ctcaggaaga taaaaggata agatttatca tcgcgtagca
    13141 aaacgttgta acgcggctga tagagtttga tgtagttgtg ttccagcaac agcgcttcgg
    13201 tttctgtatg agtaaccgtt acatcaattt gctggatctg ggcgaccagc gcttcggttt
    13261 tgcgcgaagc gaggttgcta cggaaatagc tggaaagccg ttttttcagg tctttcgctt
    13321 tgccgacata gataaccgta ccaccagcat cgtacatgcg ataaacgcct ggctggctgg
    13381 ttacggtttt taaaaacgct tttgcgtcaa actgatcact cactgacttg ataatgtctc
    13441 cgcattacac agaccatggc gaattgccag gtgagtcagc tcaacatcgc catgaatgtt
    13501 tagtttactg aacatacgat agcggtagct gttcaccgtt ttcggactga gattgagctg
    13561 ttctgagatc tcattgacct tctggccctt ggtgatcatc agcataatct gcaattcacg
    13621 ttcagacaaa ctggcaaatg ggctttctgt tttttctggt tcgatctggc ttaacgccat
    13681 ttgttgagcg atgtcagaag caatgtaacg ctgccctgaa tagacagaac gaatcgcact
    13741 cacgacttcc tgcggagccg cgcctttgct gaggtagccc gcagcaccgg cctgcatgac
    13801 tttcgctggt aaagggtttt ctgtatggac ggtaagcatg atgattttga catcagctgt
    13861 ggaacgcgcg attttacgcg tcgcctcaag accgccaatg cccggcatac tcatgtccat
    13921 tagcaccacg tcaacggcat ttgcccgaca ccacttaacg gcgtcttcac cgcacgatgc
    13981 ctcaccgacg acttttatac cctttatatc ttccagaatg cgtcgtatcc ctgcgcgcac
    14041 cagttcgtgg tcatcaacaa gtagaacgtt gatcaaagga atatctccag aaatagggat
    14101 aacgctactg atagttagtc attcgtatat tagcggtttt tattgcaact ttgaaacgtt
    14161 aaaaatggtg ggtattcgat ttttctctcg tttttgtcaa ttcgattgtc cacagttatg
    14221 gtcacgcccg tcaccaacgg cttaccggat cgtttcacag taagccctgc gacgaaatgt
    14281 gacaaaaatt acctttattc agcaaaaatg aaaatcagcc cgctgaatat gtaacattaa
    14341 ttaacccgaa caatacgggc aaaaaacatt catgataaaa atatttattg gtcattatat
    14401 taacgcattt aatagcaccg ttgacgatac gctcaaaaaa caaccactgc tatttttagc
    14461 aaagcttatg gtatactccg ccgccttaac attattcacc gcaaattttc attgcaacat
    14521 gacgaggaaa ataaatgagt acgcctgatt tttctactgc cgagaataat caagaactgg
    14581 caaatgaagt ctcctgcctg aaagcgatgc tgacgctgat gctgcaggcg atgggacaag
    14641 ctgacgcggg ccgcgtgatg ttaaagatgg aaaaacagct tgcgctgatc gaagacgaaa
    14701 cccaggctgc agtattttcc aaaacggtta agcaaattaa acaagcctac cgtcagtaat
    14761 ataaaaccgg ctgatagcgt gcctttcagc cggtttttgc atctggcacg caggacagaa
    14821 aagagatcaa attaagccag tagcggccgc gtaacaggca acctgggtct tattcggtgc
    14881 attgattttt ttctgcatgt ttttctgatg gaaattgacc gtattctcag agattgacaa
    14941 aatcatcgct atctctgctg atgtcttccc ttccgccgtc cacctcagaa tttctttttc
    15001 gcgcttgctg aaattcatct ctggcgtcat cactatttca tcatttaaac gcatcagagc
    15061 catcagactt tcgcgcacca gtaactgcat ttttaattgc agttcatcac taagaatggg
    15121 tatttcgcgc gcgctgcaac gggaaaagga caaaaagccc agcgcccggt ttggcagcat
    15181 taaatactga gtgacaccgc ggcgtaaacc atgtgcgcgc gcggcttccc ataacggctg
    15241 tgcttcgttg aataagtcat cattccacat tagatggccc tgactaaagt tttcagggtt
    15301 cagcaccgga tcaattgcga gaaagttttt tgcctgataa taactaaccc acgcctcagg
    15361 gtaattggtg tagaaagcca ctttaggtcg cgtgaatggt accgggtggc gaacacataa
    15421 cgaatagtaa tcgtactcca gctgctgagc ctgaagctct atttcatggt agacctcttc
    15481 tgcggcctcc atcctctgaa aacgcaacag catcgtgcga cgccagctga aaaaatccgt
    15541 atcctgcata gtaaaccgca acgcccctga gagtgagtat catttataat gataattcaa
    15601 actatcacat aaata
//
LOCUS       DAZGJW010000108        15594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZGJW010000108 DAZGJW010000000
VERSION     DAZGJW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.108"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1226)
                     /locus_tag="ACHKIK_003473"
     CDS             complement(<1..1226)
                     /locus_tag="ACHKIK_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9084580.1"
                     /translation="MKLNARQVETAKPKEKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPMVTLAQARAKRDEAKKLLVQGIDPKAEQKEAQAENSGAYTFET
                     IAREWHASNKRWSEDHRSRILRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFSEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRIPAKREEIKGVRYSYR
                     GMKMKEEHIVPLSRQAMVLLEQLKQISGDKELLFPGDHDAAKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHTERNNVRAAYIHTSEHLDERRL
                     MVQWWADFLEANRNNSV"
     gene            complement(1390..1474)
                     /locus_tag="ACHKIK_003474"
     tRNA            complement(1390..1474)
                     /locus_tag="ACHKIK_003474"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1438..1440),aa:Leu,seq:caa)
     gene            1670..2689
                     /gene="ahr"
                     /locus_tag="ACHKIK_003475"
     CDS             1670..2689
                     /gene="ahr"
                     /locus_tag="ACHKIK_003475"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO9084581.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDTC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGTDKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2819..4321
                     /gene="yjgR"
                     /locus_tag="ACHKIK_003476"
     CDS             2819..4321
                     /gene="yjgR"
                     /locus_tag="ACHKIK_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO9084582.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDLMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMISELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASIEQQNNPPAKGKEIAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(4440..5522)
                     /gene="lptG"
                     /locus_tag="ACHKIK_003477"
     CDS             complement(4440..5522)
                     /gene="lptG"
                     /locus_tag="ACHKIK_003477"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO9084583.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5522..6622)
                     /gene="lptF"
                     /locus_tag="ACHKIK_003478"
     CDS             complement(5522..6622)
                     /gene="lptF"
                     /locus_tag="ACHKIK_003478"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO9084584.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMCTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6685..6735)
                     /gene="ytgA"
                     /locus_tag="ACHKIK_003479"
     CDS             complement(6685..6735)
                     /gene="ytgA"
                     /locus_tag="ACHKIK_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO9084585.1"
                     /translation="MSIIILATISTTQENK"
     gene            6889..8400
                     /gene="pepA"
                     /locus_tag="ACHKIK_003480"
     CDS             6889..8400
                     /gene="pepA"
                     /locus_tag="ACHKIK_003480"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO9084586.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8754..9197
                     /gene="holC"
                     /locus_tag="ACHKIK_003481"
     CDS             8754..9197
                     /gene="holC"
                     /locus_tag="ACHKIK_003481"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO9084587.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTC
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9197..12052
                     /gene="valS"
                     /locus_tag="ACHKIK_003482"
     CDS             9197..12052
                     /gene="valS"
                     /locus_tag="ACHKIK_003482"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO9084588.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12106..13302)
                     /gene="yjgN"
                     /locus_tag="ACHKIK_003483"
                     /pseudo
     CDS             complement(12106..13302)
                     /gene="yjgN"
                     /locus_tag="ACHKIK_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
     gene            13495..13998
                     /gene="yjgM"
                     /locus_tag="ACHKIK_003484"
     CDS             13495..13998
                     /gene="yjgM"
                     /locus_tag="ACHKIK_003484"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9084589.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(14044..14460)
                     /gene="rraB"
                     /locus_tag="ACHKIK_003485"
     CDS             complement(14044..14460)
                     /gene="rraB"
                     /locus_tag="ACHKIK_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO9084590.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14622..>15594
                     /gene="argF"
                     /locus_tag="ACHKIK_003486"
     CDS             14622..>15594
                     /gene="argF"
                     /locus_tag="ACHKIK_003486"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO9084591.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGAHVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTI"
BASE COUNT         3977 a         3967 c         4066 g         3584 t
ORIGIN      
        1 acagagttat ttcgattggc ctcaagaaaa tcagcccacc attgaaccat taatctacgt
       61 tcgtctaaat gctcagaggt atgaatatat gctgcacgca cattgttacg ctctgtatga
      121 ctcaactgcc gttctatcgc gtcatcgctc cataagcctg attcaccaag cgcgccacgc
      181 gccatcgtcc taaatccatg cccacagacc tcagttttcg tgtcataccc catcgcacgc
      241 aaggcgctat ttactgtgtt ctcactcata actttggccg cgtcgtgatc ccctggaaaa
      301 agtaactctt tatcgccact aatctgcttg agttgctcta acaaaaccat cgcctgtcga
      361 ctaagaggaa cgatatgctc ctctttcatc ttcatgccac gatacgagta acgcacgcct
      421 ttaatttctt ctcgctttgc aggtatacgc caaagagatt tatcgaagtc gaactcatcc
      481 catcgcgcga aacgtaactc actggaacgt acaaaagtta gtaaggaaag ctcgaccgcg
      541 atccttgtca ttacacgacc acgatatgca gcaagacgtg caagaaactc agagaagcgg
      601 ctagagggta aagctggata gtgtcgtgct ttggttgtag ataacgcacc ggccatgtca
      661 ctggctggat tagagtcgat gtaatcgttc tgtacggcat aacgcataat ggccgtgacg
      721 cgctgttgca ggcgctgagc gacatcgtgt ttgccactgg catcaacttt tttaatcggg
      781 gctaacaggt ggctggtttt aagctgccga atgtcggacg aaccgatatg aggaaaaata
      841 taaagctcaa gatagcgcag aatgcgtgat cgatggtctt cactccagcg cttattactg
      901 gcatgccatt cacgagcaat tgtttcgaaa gtgtatgcgc ccgaattctc agcctgagct
      961 tctttctgct cggcttttgg gtcaatgccc tgcactaaga gctttttagc ctcgtcacgc
     1021 tttgctcttg cctgagcaag tgtcaccatg ggccaaacac caaaagcgag gcgatcctct
     1081 tttttgtcag aggggcgtct gtatttcatg cgccagtatt tagaaccctt agccgaaacc
     1141 tcgagataca aaccgccgcc atcggccatt ttgtaggttt tctcttttgg ctttgctgtc
     1201 tcgacctgtc tggcgttgag cttcatttgg gggcacattt ctaatcgaag ttaagatgcc
     1261 cccaattatg cccccaatag catccggatt tcaacggact agctcggaca gctcaggaca
     1321 gaaaaaccgc tacaggcatt gattttaaag ggtttgctgg attttctcgg atggtcttgg
     1381 aagtactaat ggtgccgaag gccggactcg aaccggcacg tatttctacg gttgattttg
     1441 aatcaactgc gtctaccgat ttcgccactt cggcactgaa gaggtatgcg gaaaacgttg
     1501 tggattatac ctgtcgcacg ccaccatgca agcgccagct tgcacccacc tcgctaagtg
     1561 ctgaaatttt cagcattaca ctcagcgata tgtaaccttt gtcacactcc aggcaccccg
     1621 ccctgccatg ctctacactt cccaaacaac accagagaag gacccaaaaa tgtcgatgat
     1681 aaaaagctat gccgcaaaag aagcgggcgg cgaactggaa gtttatgagt acgatcccgg
     1741 tgagctgaag ccacaagatg ttgaagtgca ggtggattac tgcgggattt gtcattccga
     1801 tctgtcgatg atcgataacg aatggggatt ttcacaatat ccgctggttg ccgggcatga
     1861 ggtgattggg cgcgtggtgg cactcgggag cgccgcgcag gataaaggtt tgcaggtcgg
     1921 tcagcgtgtc gggattggct ggacggcgcg tagctgtggt cactgcgaca cctgtattag
     1981 cggtaatcag atcaactgcg agcaaggtgc ggtgccgacg attatgaatc gcggtggctt
     2041 tgccgagaag ttgcgtgcgg actggcaatg ggtgattcca ctgccagaaa atattgatat
     2101 cgagtccgcc gggccgctgt tgtgcggcgg tatcacggtc tttaaaccac tgttgatgca
     2161 ccatatcact gctaccagcc gcgttggggt aattggtatt ggcgggctgg ggcatatcgc
     2221 tataaaactt ctgcacgcaa tgggatgcga ggtgacggcc tttagttcta atccggcgaa
     2281 agagcaggaa gtactggcga tgggtaccga taaagtggtg aatagccgcg atccgcaggc
     2341 actgaaagcc ctgtcggggc agtttgatct cattatcaat actgtgaacg tcagcctcga
     2401 ctggcagcct tattttgagg cgctgaccta tggcggtaat ttccatacgg tcggtgcggt
     2461 tctcacgccg ctgtctgttc cggcctttac gttaattgcg ggcgaccgca gcatctctgg
     2521 ttctgctacc ggcacgcctt atgagctgcg taagctgatg cgttttgccg cccgcagcaa
     2581 ggttgcgccg accaccgaac tgttcccgat gtcgaaaatt aacgacgcca tccagcatgt
     2641 acgcgatggt aaagcgcgct accgcgtggt cctgaaagcc gacttctgat ccctttgccg
     2701 ggagcctgtc gcccggcact taaatctctt tggaaggcgt ctcgcaatct gctgaattta
     2761 gcggtgaaga gtgcacagcc gttgcctata ctccaggcag gaaactggag gaaatctcat
     2821 gagcgaaccc ctgttaattg cccgcacgcc ggacacagaa ctgtttttac tgccgggaat
     2881 ggctaaccgt cacgggctga ttactggcgc aacggggacg ggtaaaactg tcacgctgca
     2941 aaaactggcg gagtctctat cggaaatcgg cgtgccggtg tttatggccg atgtgaaagg
     3001 cgatctgacc ggtatcgcgc aggcaggaac ggcgtcggaa aaactgctcg caaggcttaa
     3061 aaatatcggc gtaaatgact ggcaaccgca tgctaatccg gtggtggtgt gggatatctt
     3121 tggcgagaaa ggccatccgg tgcgggcgac ggtttcggat ctggggccgc tgttgctggc
     3181 gcggctgttg aatctcaacg atgtgcaatc tggcgtgctg aatatcatct tccgaattgc
     3241 tgacgatcag gggctgttac tgctcgactt taaagatctg cgggcgatta cccagtacat
     3301 cggcgataac gccaaatctt tccagaatca gtacggaaat atcagtagcg catcggttgg
     3361 tgccatccag cgcgggctgt tgtcgctgga acagcaaggc gcagcacact tctttggtga
     3421 gccgatgctg gatatcaaag acttgatgcg caccgatgcc aacggtaaag gcgttattaa
     3481 tatcctcagc gccgagaagc tttatcagat gccgaaactg tacgccgcca gcctgctgtg
     3541 gatgatttca gagttgtatg aacaattgcc ggaagcgggc gatctggaga agccaaaact
     3601 ggtgtttttc ttcgacgaag cacatctgct gtttaacgat gcaccgcagg tactgctgga
     3661 taagattgag caggtgataa ggcttattcg ctcaaaaggc gtgggcgtct ggttcgtttc
     3721 gcaaaacccg tctgatattc cggataatgt gctcggccag ctcggtaatc gcgttcagca
     3781 cgctttgcgg gcttttacgc caaaagatca gaaagcggta aaagctgcgg cgcaaaccat
     3841 gcgggccaat ccggcgtttg ataccgaaaa ggcaatccag gaactgggga ccggcgaggc
     3901 gttaatctcg tttctcgatg caaaaggaag tccttctgtg gtggaacggg cgatggtgat
     3961 tgcgccttgt tcgcggatgg gaccggtgac ggaagatgag cgcaatggct tgattaacca
     4021 ctccccggtg tatggcaagt acgaggatga cgtggaccgg gaatccgcct atgagatgtt
     4081 gcaaaaaggc gtacaggcga gtattgaaca gcaaaataat cctccagcga aagggaaaga
     4141 gatagcggta gatgacggca ttcttggtgg attgaaggat attttgttcg gcactaccgg
     4201 accacgcggc gggaagaaag atggtgtggt gcagacaatg gccaaaagcg ccgctcgcca
     4261 ggtgacgaat cagattgtac gcgggatgtt ggggagtttg ctgggaggga gaagaaggta
     4321 agtcaggcgc gatctgtccc cctcacccta accctctccc caaaggggcg aggggaccga
     4381 tcgaactcgt atttgacttt atcaacaatg tgaaagggag ctttcgctcc cttgcttggt
     4441 tacgattttc tcattaacag ccacaggctg attaagaaga agctggcgct tggcaacagt
     4501 gcgccgataa tcggcgggat gccgtaaacc aacgtcagcg ggccgaagat ctggtccagt
     4561 acgtagaaga caaaaccgaa gctgataccg gtgaccacac gcacgcccat cggtacgcta
     4621 cgcagtgggc caaagatgaa cgacagcgcc atcagcatca tcaccgccac ggatagcggc
     4681 tggaagattt tgctccacat attgagctga taacgtccgg catcctgacc gctcgacttc
     4741 agatacttca cgtagttgtg caaaccgctg attgagagtg catccgggtc cagcgccacc
     4801 acgcccagtt tgtctggcgt gaggttggtt ttccaggtgc cgctcaccgt ctgcgaaccg
     4861 gtgatctgtt tcggattggt cagatcagat tcatcaacct gcgacagacg ccagacttta
     4921 tgttccgggt caaacttcgc agtagcggca tagcgtacgg attgcagacg acgattttcg
     4981 ttaaaggcat aaatgctgat gccacctaac tcttcgtcac ctttaacccg ctcaatgtag
     5041 acgaagttgt tgccatcttt cgcccataag ccttgctggg tagagagcaa cgagccgccg
     5101 tacatcgcct gcgcacggta gttacgcgcc atctgctcgc cctgcggcgc gacccattcg
     5161 ccaatcgcca tcgtcagcaa gaccagcgga atggcggttt tcatcaccga cagcgccacc
     5221 tgcatacggg taaaaccaga agcctgcatc accaccagtt cgctgcgctg cgccagcatc
     5281 ccaagaccaa gcaacgcccc aagcagagcc gccatcggga agaagatttg cacatctttc
     5341 ggcacgctca gcaaggtata cattcctgcg cctaacgcgt cgtaactccc ctgcccggct
     5401 tttttcaact gatcgacaaa tttgataatg cccgacagcg acaccagcat gaacagcgtc
     5461 atcatgatgg tggtgaaaat agttttaccg atatagcggt caagtacgcc aaaaggttgc
     5521 atcacaccgc tcctttacgc gaaaaactgg cgcgcaggcg gcggaccggc acggtgtccc
     5581 aaaggttgag aacaatcgcc aaagccagat aaatcaggtt aacggtccac atccacagcg
     5641 tcgggtccag cttaccttta ccgccgttcg atttcaggga ggtctggatc aggaagaaaa
     5701 gtagatacag cagcatggct ggcagcatcg acagtacgcg tccctgacgt gggttaacca
     5761 cgctcagcgg tacgaccata agtgccatca taaacacggt gaataccaac gtgatacgcc
     5821 agttcagttc tgcgcgagca cgatcggtgt cagtgttcca caatgtgcac atgtccatct
     5881 ggtcggtatc gttcgggtcg agcgccaccg cctggtgacc aatgatcgcc tgataatcct
     5941 ggaaatccgt aatgcggaaa tcacgtaaca gtgcagtgcc ttcgaagcgc gttccctggt
     6001 tgagagtgac gacctgggag ccgtcgcgca gctgggttaa atgtccggaa tcggccacca
     6061 ccacagaagg acgtgcatta ccttttggtc gaatttgtgc gaggaacaca tctttgaaat
     6121 cgctgccgtc aacgctttcg atgaacagca ccgagctgcc attagtcgct tgctggaatt
     6181 gcccttgcgc cagcgccgcc atgccagggt tcgctttcgc ttctgctaac acttcatcct
     6241 gatgacgcga tgaccacggt cccgcccaca tcacgttaac cgccgcgacg attgccgtga
     6301 ataccgcaag gatcattgcc gctttcacca gaaccgcttt gctcaggccg caggcatgca
     6361 ttaccgtaat ttcactttcg gtatacagtt tgcccagcgt catcagcagc ccgaggaaca
     6421 ggcttaatgg caggataagc tgcgccattt ccggcacgcc caacccgaga agggagagca
     6481 ccagattcgc cggaatatcg ccgtcaaccg ctgcgccgag gatcctcact aacttttgac
     6541 agaagaagat caaaagcaag atgaagagta tcgccagctg gcttttgagc gtctcccgca
     6601 ccagatatct tatgattatc actttaaata cgcccgtaaa aactcgtctt ttgcaggatt
     6661 ttagcttgtt tcatggctta aacgtcattt attctcttga gtcgtcgaaa tcgtcgctaa
     6721 gataattata ctcaacggat tcacctctca gattttgttc tgacgtgcca atgccgtaat
     6781 aacgttaaga ttaacacgaa gtcatcgcaa cagcggacat gagttacgaa agcttgcaat
     6841 tctatctgta gccaccgccg ttgtctttaa gattcaggag cgtagtgcat ggagtttagt
     6901 gtaaaaagcg gtagcccgga gaaacagcgg agtgcctgca tcgtcgtggg cgtcttcgaa
     6961 ccacgtcgcc tttctccgat tgcagaacag ctcgataaaa tcagcgatgg gtacatcagc
     7021 gccctgctac gtcggggcga actggaagga aaaccggggc agacattgtt gctgcaccat
     7081 gttccgaatg tactttccga gcgaattctc cttattggtt gcggcaaaga acgtgagctg
     7141 gatgagcgtc agtacaagca ggttattcag aaaaccatta atacgctgaa tgatactggc
     7201 tcaatggaag cggtctgctt tctgactgaa ctgcacgtta aaggccgtaa caactactgg
     7261 aaagtgcgtc aggctgtcga gacggcaaaa gaaacgctct acagtttcga tcagctgaaa
     7321 acgaacaaga gcgaaccgcg tcgtccgctg cgtaaaatgg tgttcaacgt gccgacccgc
     7381 cgtgaactga ccagcggtga gcgcgcgatc cagcacggtc tggcgattgc cgccgggatt
     7441 aaagcagcaa aggatcttgg caatatgccg ccgaatatct gtaacgccgc ttacctcgct
     7501 tcacaagcgc gccagctggc tgacagctac agcaagaatg ttatcacccg cgttatcggc
     7561 gaacagcaga tgaaagagct ggggatgcat tcctatctgg cggtcggtca gggttcgcag
     7621 aacgaatcgc tgatgtcggt gattgagtac aaaggcaacg cgtcggaaga tgcacgccca
     7681 atcgtgctgg tgggtaaagg tttaaccttc gactccggcg gtatctcgat caagccttca
     7741 gaaggcatgg atgagatgaa gtacgatatg tgcggtgcgg cagcggttta cggtgtgatg
     7801 cgtatggtcg cggagctaca actgccgatt aacgttatcg gcgtgttggc aggctgcgaa
     7861 aacatgcctg gcggacgagc ctatcgtccg ggcgatgtgt taaccaccat gtccggtcaa
     7921 accgttgaag tgctgaatac tgacgctgaa ggccgcctgg tactgtgcga cgtgttaact
     7981 tacgttgagc gttttgagcc ggaagcggtg attgacgtgg cgacgctgac cggtgcctgc
     8041 gtgatcgcgc tgggtcatca cattaccggt ctgatggcga accataatcc gctggcccat
     8101 gaactgattg ccgcgtctga acaatccggt gaccgcgcat ggcgcttacc gctgggtgac
     8161 gagtatcagg aacagctgga gtccaatttt gccgatatgg cgaacattgg cggtcgtcct
     8221 ggtggggcga ttaccgcagg ttgcttcctg tcgcgcttta cccgtaagta caactgggcg
     8281 cacctggata tcgccggaac cgcctggcgt tctggtaaag caaaaggcgc aaccggtcgt
     8341 ccggtagcgt tgctggcaca gttcctgttg aaccgcgctg ggtttaacgg cgaagagtaa
     8401 ttgcgtcagg caaggctgtt attgccggat gcggcgtgaa cgccttatcc gacctacaca
     8461 gcactgaact cgtaggcctg ataagacgcg caagcgtcgc atcaggcaat gctgttattg
     8521 tcggatgcgg catgaacgcc ttatccgacc tacacagcac cgaactcgta ggcctgataa
     8581 gacacaacag cgtcgcatca ggcaatgctg ttattgtcgg atgcggcgtg agcgccttat
     8641 ccgacctaca cacagcactg aactcgtagg cctgataaga cacaacagcg tcgcatcagg
     8701 cgctgcggtg tatacctgat gcgtatttaa atccaccaca agaagcccca tttatgaaaa
     8761 acgcgacgtt ctaccttctg gacaatgaca ccaccgtcga tggcttaagc gccgttgagc
     8821 aactggtgtg tgaaattgcc gcagaacgtt ggcgcagcgg taagcgcgtg ctcatcgcct
     8881 gtgaagatga aaagcaggct taccggctgg atgaagccct gtgggcgcgt ccggcagaaa
     8941 gctttgttcc gcataattta gcgggagaag gaccgcgtgg cggtgcgccg gtggagatcg
     9001 cctggccgca aaaacgcagc agcagcccgc gcgatatatt gattagtctg cgaacatgct
     9061 ttgcagattt tgccaccgct ttcacagaag tggtagactt cgttccttat gaagattctc
     9121 tgaaacaact ggcgcgcgaa cgctataaag cctaccgcgt ggctggtttc aacctgaata
     9181 cggcaacctg gaaataatgg aaaagacata taacccacaa gatatcgaac agccgcttta
     9241 cgagcactgg gaaaagcagg gctactttaa gcctaatggc gatgaaagcc aggaaagttt
     9301 ctgcatcatg atcccgccgc cgaacgtcac cggcagtttg catatgggtc acgccttcca
     9361 gcaaaccatc atggatacca tgatccgcta tcagcgcatg cagggcaaaa acaccctgtg
     9421 gcaggtcggt actgaccacg ccgggatcgc tacccagatg gtcgttgagc gcaagattgc
     9481 cgcagaagaa ggtaaaaccc gtcacgacta cggccgcgaa gctttcatcg acaaaatctg
     9541 ggaatggaaa gcggaatccg gcggcacgat tacccgtcag atgcgccgtc tcggcaactc
     9601 cgtcgactgg gagcgtgaac gcttcacgat ggacgaaggc ctgtccaatg cggtgaaaga
     9661 agttttcgtt cgtctgtata aagaagacct gatttaccgt ggcaaacgcc tggtaaactg
     9721 ggacccgaaa ctgcgcaccg ctatctctga cctggaagtg gaaaaccgcg aatcgaaagg
     9781 ttcgatgtgg cacatccgct atccgctggc tgacggtgcg aaaaccgcag acggtaaaga
     9841 ttacctggtg gttgcgacta cccgtccgga aaccctgctg ggcgataccg gtgtagccgt
     9901 taacccggaa gatccgcgtt acaaagacct gattggcaaa tatgtcattc taccgctggt
     9961 taaccgtcgt attccgatcg ttggcgacga acacgccgac atggaaaaag gcaccggctg
    10021 cgtgaagatc accccggcgc acgactttaa cgactatgaa gtcggtaaac gtcacgccct
    10081 gccgatgatc aacatcctga cctttgacgg cgatatccgt gaaagcgccc aggtgttcga
    10141 taccaaaggt aacgaatctg acgtttattc cagcgagatc ccggcagaat tccagaaact
    10201 ggagcgtttt gccgcacgta aagcagtcgt tgctgcggtt gacgcactcg gcctgctgga
    10261 agaaattaaa ccgcacgacc tgaccgttcc ttacggcgac cgtggcggcg tagttatcga
    10321 accaatgctg accgaccagt ggtacgtgcg tgccgatgta ctggcgaaac cggcggttga
    10381 agcggttgag aacggcgaca ttcagttcgt accgaagcag tacgaaaaca tgtatttctc
    10441 ctggatgcgc gatattcagg actggtgtat ctctcgtcag ctgtggtggg gtcaccgtat
    10501 cccggcatgg tatgacgaag cgggtaatgt ttatgttggc cgcaacgaag aagaagtgcg
    10561 caaggaaaat aacctcggtg ccgacgttgc cctgcgtcag gacgaagacg ttctcgacac
    10621 ctggttctct tctgcgctgt ggaccttctc tacccttggc tggccggaaa acaccgacgc
    10681 cctgcgtcag ttccacccaa ccagcgtgat ggtatccggc ttcgacatca tcttcttctg
    10741 gattgcccgc atgatcatga tgaccatgca cttcatcaaa gatgaaaatg gcaaaccgca
    10801 ggtgccgttc cacactgttt acatgaccgg tctgattcgt gacgacgaag gccagaaaat
    10861 gtccaaatcc aagggtaacg ttatcgaccc gctggatatg gttgacggta tttcgctgcc
    10921 agaactgctg gaaaaacgta ccggcaatat gatgcagccg cagctggcgg acaaaatccg
    10981 taagcgcacc gagaaacagt tcccgaacgg cattgagccg cacggcaccg atgccctgcg
    11041 cttcaccctg gcggcgctgg cgtctaccgg tcgtgacatc aactgggata tgaagcgcct
    11101 ggaaggttac cgtaacttct gtaacaagct gtggaacgcc agccgctttg tgctgatgaa
    11161 cacagaaggt caggattgcg gctttaacgg cggcgaaatg acactgtcgc tggcggaccg
    11221 ctggattctg gcagagttca accagaccat caaagcgtac cgcgaagcgc tggacagctt
    11281 ccgcttcgat atcgctgcag gcattctgta tgagttcacc tggaaccagt tctgtgactg
    11341 gtatctggag ctgaccaagc cggtaatgaa cggtggcacc gaagccgaac tgcgcggtac
    11401 tcgccatacg ctggtgactg tactggaagg tctgctgcgc ctcgcgcatc cgatcattcc
    11461 gttcatcacc gaaaccatct ggcagcgtgt gaaagtactt tgcggtatca ctgccgacac
    11521 catcatgctg cagccgttcc cgcagtacga tgcatctcag gttgatgaag ccgcactggc
    11581 cgacaccgaa tggctgaaac aggcgatcgt tgcggtacgt aacatccgtg cagaaatgaa
    11641 catcgcgccg ggcaaaccgc tggagctgct gctgcgtggt tgcagcgcgg atgcagaacg
    11701 tcgcgtaaat gaaaaccgtg gcttcctgca aaccctggcg cgtctggaaa gcatcaccgt
    11761 gctgcctgcc gatgacaaag gtccggtttc cgttacgaag atcatcgacg gcgcagagct
    11821 gctgatcccg atggctggcc tcatcaacaa agaagatgag ctggcgcgtc tggcgaaaga
    11881 agtggcgaag atcgaaggcg aaatcagccg tatcgagaac aagctggcga atgaaggctt
    11941 tgtcgcccgc gcaccggaag cggtcatcgc gaaagagcgt gagaagctgg aaggctatgc
    12001 ggaagcgaaa gcgaagctga ttgaacagca ggctgttatc gccgcgctgt aatcaacacc
    12061 gtgatgaaaa cgaaggccgg agaatgctcc ggcctttttt atctcttaca gaaatggtaa
    12121 agaaggcata attccccgtg aaatactggc taagaagcct ttatctggtt gttcttcttt
    12181 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    12241 aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg atatcactac
    12301 caacgcacac atgcgataaa gcataccgtg gtaagttaaa gttgagcgaa aacgaatacg
    12361 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    12421 ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt tacgttgcat
    12481 ttccataaat tgctgtaaat tctcaatatc atcatttgca tacacagaac tatcatacgc
    12541 atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagctaa
    12601 aaatgcgagg atggcatatt ttatacaata cgcagtatct aatttcacct tgaaacgatg
    12661 tataccgaaa ctggtattgc tccagagaaa actcattacc aggctatata aagtaccatt
    12721 aaaaatacca atggaaacaa taccaactat tgccatcaga accacagata taataacact
    12781 acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaat
    12841 ggccattaaa atcggcaaga aaaaggttac tcaccagaac cctttcatag agcaattaaa
    12901 actaaatctt acgccgttga gactagtcat caaggcctga taacgcagtc cttttgctgc
    12961 cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa gcggcatatc
    13021 tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa
    13081 aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacctcca tattagcata
    13141 gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt
    13201 taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa
    13261 cgaatgggac ggaacatcca tttcattaat aacttgagcc ataaaaattc cctgaattaa
    13321 aaaatccatt gatataagtt atagatacag acagtaaaat gttctgccac gttacaatac
    13381 tcttatcagt aataggctgg caaaaacttt attgctttat aagatgcttg cgcacgtaag
    13441 aagagagtgg tattaaacaa atgtataatt actttatttt tattgggtta aactatgaat
    13501 aacattgcgc cgcaatcacc tgtaatgcgt cgcctgacgc tgcaggataa tcctgctatt
    13561 gcccgcgtca ttcgtcaggt atccgccgaa tacggtctta ccgctgataa aggctacacc
    13621 gtcgccgatc cgaatcttga cgagctgtat caagtgtata gtcagccggg tcatgcatat
    13681 tgggtggttg agtacgaggg tgaagtggtc ggcggcggtg ggattgcgcc attagcaggt
    13741 agtgaatcgg acatttgcga actgcaaaag atgtattttc ttccggctat tcgcggtaaa
    13801 gggctggcga aaaaactggc cttaatggcg atggagcagg cacgagagat gggcttcaaa
    13861 cgctgctatc tggaaacgac cgctttttta aaggaagcca ttgcgcttta tgagcatttg
    13921 ggctttgagc atatcgacta tgcgcttggc tgcacgggcc atgtcgattg tgaagtgcgg
    13981 atgctacgcg aactctaaaa aaatgcccgc tagtgtgtgc tatcgggcat tttaatcaat
    14041 taattagtgg cgaaccccgt catcgtcttc atcgacaaaa tcttcatcgt cgccgtcttc
    14101 gccgttggga tcttcaaagt aagtgcccca accgtcgtac tcaacgtcaa atttctccgc
    14161 cagcgtcatc agttgttcaa cctgggcatc gatcagatcg gcattcaacg cgcactcgct
    14221 gaggatgtcg cagcaaatca cgatatcacc atcttcaact tccagctctt ctggatcggt
    14281 cacttcgtaa ccgagtttaa acgcttcaac tgctgctttt tccagggttt ccagatcgtc
    14341 tgcggaaaga tggtgttcga tggtatacag cgcgtccgga tcgctgccat cttccagtaa
    14401 ttcttcaata atcaaacgtg tttcttcacg ctgttcttcc agttgttccg ggtttgccat
    14461 ggctcattcc tcttaatgtc ctgccgatac tcttattgtc acacaccact gacattgcct
    14521 ccacctttgt gacaaagatt tatgctttag acttgcaaat gaataatcat ccatataaat
    14581 tgaattttaa ttcattgagg tgttagccac aggagggatc tatgtccggg ttttatcata
    14641 agcatttcct gaaattactc gatttcacgc cagctgaact caacagcctg ctgcagttag
    14701 ccgcgaagct gaaagccgat aagaaaagcg gcaaagaaga agccaaactc actggtaaaa
    14761 acatcgcgct catcttcgaa aaagactcga cccgtacccg atgctctttc gaagttgccg
    14821 catatgacca gggtgctcac gttacttatc tcggcccaag cggcagtcag attggtcata
    14881 aagagtcgat taaagacact gcccgcgtgc tgggtcgcat gtatgacggt attcagtatc
    14941 gcggctatgg tcaggagatt gtcgaaacac tggcggaata cgctggcgtg ccggtatgga
    15001 acggcctgac caatgagttt caccccacgc agttgctggc ggatctcctc accatgcagg
    15061 agcatttacc cggtaaagca ttcaacgaaa tgacgctggt ctatgcaggt gacgcacgta
    15121 acaacatggg caattcgatg ctcgaagctg cggcgcttac cggtctggat ttgcgtctgg
    15181 tcgcgccaca ggcatgctgg ccggaagctg cgctggttac ggaatgccgc gctctggcac
    15241 agcaaaatgg cgggaatatt acgctgacgg aagatgtagc taaaggagtt gaaggtgctg
    15301 actttatcta taccgatgta tgggtgtcga tgggtgaagc aaaagagaaa tgggctgaac
    15361 gcattgcatt gctgcgtgat tatcaagtga acagcaagat gatgcagttg accggtaacc
    15421 cggaggtcaa attcctccac tgcctgcccg cgtttcatga cgaccaaacg acgcttggca
    15481 agaaaatggc ggaagagttt ggcctacatg gcggaatgga agtgactgat gaggtcttcg
    15541 aatctgccgc cagcattgta tttgatcagg cggaaaaccg catgcatact atca
//
LOCUS       DAZGJW010000109        15466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZGJW010000109 DAZGJW010000000
VERSION     DAZGJW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.109"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(45..428)
                     /gene="quuD"
                     /locus_tag="ACHKIK_003487"
     CDS             complement(45..428)
                     /gene="quuD"
                     /locus_tag="ACHKIK_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO9084592.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(514..654)
                     /gene="ylcG"
                     /locus_tag="ACHKIK_003488"
     CDS             complement(514..654)
                     /gene="ylcG"
                     /locus_tag="ACHKIK_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO9084593.1"
                     /translation="MMFEFYMAERLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(651..1013)
                     /gene="rusA"
                     /locus_tag="ACHKIK_003489"
     CDS             complement(651..1013)
                     /gene="rusA"
                     /locus_tag="ACHKIK_003489"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO9084594.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(1010..1300)
                     /gene="ybcO"
                     /locus_tag="ACHKIK_003490"
     CDS             complement(1010..1300)
                     /gene="ybcO"
                     /locus_tag="ACHKIK_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO9084595.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(1293..1463)
                     /gene="ninE"
                     /locus_tag="ACHKIK_003491"
     CDS             complement(1293..1463)
                     /gene="ninE"
                     /locus_tag="ACHKIK_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO9084596.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(1463..1918)
                     /gene="ybcN"
                     /locus_tag="ACHKIK_003492"
     CDS             complement(1463..1918)
                     /gene="ybcN"
                     /locus_tag="ACHKIK_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO9084597.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQLQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLIRRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(1915..2016)
                     /locus_tag="ACHKIK_003493"
     CDS             complement(1915..2016)
                     /locus_tag="ACHKIK_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084598.1"
                     /translation="MRTYNLNYLLPSQMQKCTCDFLYPAFDLCGGEA"
     gene            complement(2140..2541)
                     /locus_tag="ACHKIK_003494"
     CDS             complement(2140..2541)
                     /locus_tag="ACHKIK_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084599.1"
                     /translation="MGKSTLVIGRELTKDMEFSGQFLLKKLKLQNLTIDAAMWFYYPD
                     LSWRYILVISDFSERGPAEIYRKISEINRNSISKKYKPIPLEAIEAKGDSAFVYKMLK
                     GFARVNDGKVRVSNSMVNGLEIVDCLIYELK"
     gene            complement(2520..2936)
                     /locus_tag="ACHKIK_003495"
     CDS             complement(2520..2936)
                     /locus_tag="ACHKIK_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HFO9084600.1"
                     /translation="MNKNDLEALSDTRLNEAKCLLDHGFFHGAYYLCGYAVECALKAC
                     IAKSFLQHEFPNKKVVNDSYTHDLSQLLKIANLHQILIADAKNDVSLEINWSVVKDWS
                     EQFRYDNNISKAMAEQLFDAVGDQNSGVLKWVKAHW"
     gene            complement(3236..3844)
                     /locus_tag="ACHKIK_003496"
     CDS             complement(3236..3844)
                     /locus_tag="ACHKIK_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001415151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFO9084601.1"
                     /translation="MVTQLAFRDVKFTPINHNNQIWFTSKELAAALKYASTKAVTDIY
                     NKNIDEFTDGMSQVVESTTSGNYRKKTRIFSLRGAHLIAMFARTPVAKEFRRWVLDIL
                     DREIQQSPITKQFTDNELCTLAWLWRASDTMLTACQNVTPLLQVAEHREAGRFTSIEQ
                     EYPRILNKAQAILARETAHVKFRPWQDDKWSRVLTHLRSERL"
     gene            complement(4270..4338)
                     /locus_tag="ACHKIK_003497"
                     /pseudo
     CDS             complement(4270..4338)
                     /locus_tag="ACHKIK_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4597..4944
                     /locus_tag="ACHKIK_003498"
     CDS             4597..4944
                     /locus_tag="ACHKIK_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084602.1"
                     /translation="MTIKATDIEILHRYAEGVMERSNHHAKNVGAAALTLLGGVIWKA
                     LPGSIEIRTYNGSLANMVWWQSERTLKNYAISYNHNSCEIEMRDESVKGAVLFSISNE
                     TTPEKILSQLSEL"
     gene            complement(5149..5850)
                     /locus_tag="ACHKIK_003499"
     CDS             complement(5149..5850)
                     /locus_tag="ACHKIK_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO9084603.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(5847..6866)
                     /locus_tag="ACHKIK_003500"
     CDS             complement(5847..6866)
                     /locus_tag="ACHKIK_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO9084604.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGTYPNQVNTKDKLTKDNIKPYSSENSGESSDLPENDLPVVK
                     ADAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTVGKPKLDLTNTDWI
                     YGVDL"
     gene            complement(6863..7402)
                     /locus_tag="ACHKIK_003501"
     CDS             complement(6863..7402)
                     /locus_tag="ACHKIK_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO9084605.1"
                     /translation="MQPLPYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRTRQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPELAGPLMAMVTTMLGAI"
     gene            complement(7472..7702)
                     /locus_tag="ACHKIK_003502"
     CDS             complement(7472..7702)
                     /locus_tag="ACHKIK_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9084606.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            7807..8496
                     /locus_tag="ACHKIK_003503"
     CDS             7807..8496
                     /locus_tag="ACHKIK_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO9084607.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            8578..8841
                     /locus_tag="ACHKIK_003504"
     CDS             8578..8841
                     /locus_tag="ACHKIK_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084608.1"
                     /translation="MSNKTLVKKSNNCVDAYADAFGYSSFGDGSQRLGSISFFHNVTE
                     WPVDESDDVENIKYNIATIRMPEELMLKLADFIRDQHDKAKTN"
     gene            8854..9297
                     /locus_tag="ACHKIK_003505"
     CDS             8854..9297
                     /locus_tag="ACHKIK_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084609.1"
                     /translation="MKHDYYEAPNLNDKIAKAAESFCLHSSASSQLAGISGKRWDMLQ
                     SSGSSGSGSDNMPEKVAKLESDVAHIKRDVDELKTDVKAIDKNMITILARLDSIKESL
                     TNKPSSDAVDRKISDAKLAVLLGVPAIIAAGTGLYKLSMYFFFSA"
     gene            9848..10054
                     /locus_tag="ACHKIK_003506"
     CDS             9848..10054
                     /locus_tag="ACHKIK_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO9084610.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKRKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNALNH"
     gene            10130..10426
                     /locus_tag="ACHKIK_003507"
     CDS             10130..10426
                     /locus_tag="ACHKIK_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO9084611.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            10432..11217
                     /gene="bet"
                     /locus_tag="ACHKIK_003508"
     CDS             10432..11217
                     /gene="bet"
                     /locus_tag="ACHKIK_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO9084612.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            11214..11894
                     /locus_tag="ACHKIK_003509"
     CDS             11214..11894
                     /locus_tag="ACHKIK_003509"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO9084613.1"
                     /translation="MIPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            11891..12073
                     /locus_tag="ACHKIK_003510"
     CDS             11891..12073
                     /locus_tag="ACHKIK_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO9084614.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            12046..12237
                     /locus_tag="ACHKIK_003511"
     CDS             12046..12237
                     /locus_tag="ACHKIK_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO9084615.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            12314..12529
                     /locus_tag="ACHKIK_003512"
     CDS             12314..12529
                     /locus_tag="ACHKIK_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO9084616.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQHFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            12628..12846
                     /locus_tag="ACHKIK_003513"
     CDS             12628..12846
                     /locus_tag="ACHKIK_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO9084617.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRQNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            12894..13172
                     /locus_tag="ACHKIK_003514"
     CDS             12894..13172
                     /locus_tag="ACHKIK_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9084618.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            13144..13209
                     /locus_tag="ACHKIK_003515"
                     /pseudo
     CDS             13144..13209
                     /locus_tag="ACHKIK_003515"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            13371..14534
                     /gene="intD"
                     /locus_tag="ACHKIK_003516"
     CDS             13371..14534
                     /gene="intD"
                     /locus_tag="ACHKIK_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO9084619.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(14550..14626)
                     /locus_tag="ACHKIK_003517"
     tRNA            complement(14550..14626)
                     /locus_tag="ACHKIK_003517"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14590..14592),aa:Arg,seq:tct)
     gene            14869..>15466
                     /gene="fimZ"
                     /locus_tag="ACHKIK_003518"
     CDS             14869..>15466
                     /gene="fimZ"
                     /locus_tag="ACHKIK_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO9084620.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVE"
BASE COUNT         4280 a         3624 c         3511 g         4051 t
ORIGIN      
        1 ttcaacgccc cattcttaat aatttttagt aaacaattgg catattaatt agagttatta
       61 acaacgatat ccatctctaa ccggatatct aatgccatta acatcccttc aattatgccc
      121 tcagccttct gtaacctttt cccgatataa ccatcagagc agcaatgctt acctgccagt
      181 gacatgaatg tcataccgac tacataataa tctactaata aatcgtgcaa atcgctgttg
      241 ttctttttca gacgggccat gcacccgcaa atgatcatcg cgtcatcgtc acaacattgc
      301 gggcgagatt ttacttttga agtaattaat cccttaaaac cggcggcaat ggacgaccag
      361 gtcacatctt catgattatt agccgcccac gctccccaac gctcaagaac catctgaata
      421 tcacgcatca acttactcca caaaaatcag accagaacgc caattacaag caaaaatcaa
      481 cgaaacagta ttagttgatt gttatctctg acttcatact cctgctcctg tcagggtttt
      541 ggcgtaattc ttcagtattc ggtaatcggt caaaacagaa ccggggaaac gatataagcg
      601 cagacgcccc cagcggtggc gaagacgttc tgccatataa aactcaaaca tcattcattc
      661 cccatttcgg tgatggtcag ttccagcctc ccacctttgg taacaggcat cttcacaacg
      721 cggtaatcaa cgacctgagc atcatccagc cagaaacctg ctttagtgag tgcgtcaaaa
      781 gcggcttttt gcagattatc caggtcacgg cgacggcgat ccggcatgtg gcactcaatg
      841 cggattttca caggcatagc caagccgata tccagcattg cgtttttaat gattcgggcg
      901 acgttatcgc ggtatgcctg cccctctgcg ctgacgtgcg tgcgcccgcg attatggcgg
      961 taatagcgat tattgctcgg aggccagggt aatgtgatgc tgtaggtatt cacgccttga
     1021 ttaccccctc tttcagccag ataacctgcg ttctcgccat accttccagc gcgcattctt
     1081 ttgcgtactc agcatcgaca aaatgcgtgc ggcggtcgat ttcgtcgtga caggcagaac
     1141 aggcaatggt ggcaataagg tctggcggtt taataccggt gccacacagt ccagccagcc
     1201 ggatatgtgc cagtacagac gtttcagggt tgccattgca tacgccaggg attcttacct
     1261 ggcattcgcg accacgcgct gcttttctca aatcagccat gattcctcct tgctgccagt
     1321 cgcaaccatt ttttatcaac caggctggcg gtatatccga gcagtgttgg tatttcggat
     1381 ggttttagct caggcttacg cttacgacga tttggtactc tgtagatgtg tccgttcatg
     1441 acacgaataa gcggtgtagc cattacgcct cctgcttgtc gcgcagcagc tggaactcgc
     1501 agctctgcgg aatagtcagg tggcagccaa tattcatcgc ccaggcttca accttacaca
     1561 ggaagacata catctctccg gtatcaagat cggaggtatg gcgtaacgac tggatagtag
     1621 tgatttcgcc ggttacgaca tcaaccaggt ccttggtttc ataaccgagg tatgtgtgtt
     1681 tgagagcatc ttttacccat gctgcggtag cgaacgattt ccccctgcgg ataaggtatt
     1741 cactgatttc gctgtaccac atgtggctaa gtgcattctg ggaaagactg cgtctctcgc
     1801 gccacggttt aagcaccatg cgaaagcact tgccgtcttc caaataaggc tggagctgct
     1861 gaccgatagc ggtgaagtta ccgcgatgca gtttgatgcc gtcttgtggg aggttcacgc
     1921 ttcacctccg cagaggtcaa acgctggata aagaaaatcg caggtgcatt tctgcatctg
     1981 tgacgggaga agatagttta gattgtatgt gcgcataaac gtccccgttt agcgcagaag
     2041 tcaccggagg agttcaaact ccggtgactt aattatgaca agttgattat tgaaaatcaa
     2101 aagtcattta aacaataatg atacccagca agagatttct tattttaact catagatcag
     2161 acagtcaacg atttctaaac catttaccat agaattagaa acgcgaactt taccatcgtt
     2221 gactctagca aatcctttta acattttata aacaaaagct gaatccccct tagcctcaat
     2281 tgcttctaat ggtatcggct tatacttttt tgatatgcta tttctattta tctcactgat
     2341 ttttctatat atttctgccg gcccacgttc tgagaagtca ctgataacta aaatatatct
     2401 ccaagataga tctgggtaat aaaaccacat tgcagcatca atagttaaat tctgtaactt
     2461 gagttttttt aacaaaaatt gacctgagaa ttccatatct tttgtcaact ctctgccgat
     2521 taccagtgtg cttttaccca tttcaaaact ccagaatttt ggtcacctac agcatcaaac
     2581 aattgttcgg ccatagcttt acttatgtta ttgtcgtatc taaattgctc gctccagtct
     2641 ttaacgaccg accagttaat ctccaacgaa acatcatttt ttgcgtcagc aatcaaaatt
     2701 tgatgtaagt tggcaatttt gagaagttgc gacaaatcat gagtatatga atcatttacg
     2761 acttttttgt ttggaaactc atgttgtaaa aatgacttgg caatacaggc cttcaatgca
     2821 cattcaactg catacccaca aagataatat gcaccatgaa aaaagccatg atcaagcaag
     2881 catttggcct cattaagcct agtgtcagat aatgcttcaa ggtcgttttt attcatacaa
     2941 ttataacttg aattatccta atttttatga ttataccctt atcaggtatg atgtaggaac
     3001 tgaaaacaat attggatatc attttcggta tttctctgca aattccagcc cacaggatct
     3061 cggtgtacaa tctgtcgctg atcgcgcaaa tttagggtca atcacttcat tttttgcatc
     3121 aaaccctcac taacatcgaa cgattatagt acaagtagtg ttcttgactt ctcattaaac
     3181 cgtcagcatt caattaatta caaaatacta gcggcatttc tctcccgcaa ctttattaca
     3241 gccgttcaga acgtaaatgc gtcaatactc gactccactt atcatcctgc cacggccgga
     3301 attttacatg tgccgtttct ctggcaagga ttgcctgtgc cttattgagt atccggggat
     3361 attcttgttc gattgaagtg aagcgaccgg cttcgcggtg ttctgctacc tgaagaagcg
     3421 gagtgacgtt ttggcaagcg gttaacattg tgtcacttgc ccgccataac caagcaagtg
     3481 tgcaaagttc gttatcagtg aattgttttg tgattgggga ttgttgaatt tctcgatcga
     3541 gaatatccag cacccagcgg cggaattctt tggccacagg agtgcgggcg aacatggcga
     3601 tcagatgggc gccacggagg gagaaaatgc gagtcttctt acgataattt cctgaggtcg
     3661 ttgattcgac gacctgagac attccgtcag taaactcatc aatatttttg ttgtagatgt
     3721 cggttactgc tttggtgctg gcatatttta atgctgccgc caattctttg ctggtaaacc
     3781 agatctggtt gttgtgattt ataggagtga attttacatc gcggaatgcg agctgagtga
     3841 ccatagcgat aatctcgttt tgaatttgag ctatcaccac cgacgacgcc aatcgactgg
     3901 tggtggactg tgcaaggttg gcgtaaccgg tcaaaacgaa atccggcgct tccgaagaag
     3961 ccctcacaca gcccaccata atttttgtgt agacgtgcac tgcgcataaa aaaaccgcga
     4021 acgcggttat gcgtcgtttt gatgtccggg acgccaatcc cgtgtgccga ttttgcggca
     4081 acgcacagaa tatagcgccg gataaataat gtcgtcaacc cttgcatatg gggggcgcga
     4141 tgatcactac taccaccata atcgctccaa ctgttgcaac tactttaggc taagacatca
     4201 cgatttccat atcaccttta gccgccagac aaacagagac tccctgttct tggctgccaa
     4261 aaacaccgaa tgcaaaaaat cgcaggtgta tttctgcatc tgtggaggga gaagagagtt
     4321 tggattgtat gtgcgcataa acgtccccgt ttagcgcaga agtcaccgta gttgttcata
     4381 ttctgatgac atgataattt cctattgaat agaaaaaatt aacacgactc attcttatga
     4441 ctggaaaaag caatcatgct aataatcatt gttgttccag ttccaaatgc agcgaaataa
     4501 tcaccaatat tttttctacc ggtttcgact ttgatgctat attctgctaa cacctaacta
     4561 acactacgtt tcacacagca attacatgga aataacatga ctatcaaagc gactgacata
     4621 gagattcttc atcgttatgc tgaaggggta atggaacgtt ctaaccatca tgcaaaaaat
     4681 gttggtgcag ccgctcttac attgctagga ggtgttatct ggaaagccct gcccggatca
     4741 atcgagataa gaacgtataa cggaagtctt gcaaatatgg tgtggtggca aagcgaaaga
     4801 acattaaaaa attatgcaat ctcatacaac cataattcat gtgaaattga aatgagagat
     4861 gaatcagtca aaggtgctgt attatttagc atttccaacg aaaccactcc agaaaaaata
     4921 ttatcacagc tttctgagct ttaattaaag gcggtgctaa caccgcccat accattactc
     4981 cacatcttac gcaacttgat aattatgctc tattaaatac ccacaatatt tcatcaatgc
     5041 atgggtcagc tacaaaatgt ttaccgtggt aattcctgtt ttcgcaacat ctcaaaagaa
     5101 caataaacac tgtatttctt caagtaacga agaatctttc tttgcccgtc atacacttgc
     5161 tcctttcagc ccaaacttag ctttgatttc tgcgatcttc gccagagcct gtgcacgatt
     5221 tagaggtcta ccgcccataa caggaagttg ttttactggt tcaggtatcg tctcaccacg
     5281 gttaattcgc gctgtcatac aggtcagttc atcggcagcc ttgcgccgta attccgcgtc
     5341 agtcagcgca ttggcccgca tgttctggta caggttggta accaaccagt aatgcgcgtt
     5401 cgatttccac ggataagact ctgcatccgg atacaggcca cgcttccggc aatactcgta
     5461 aaccatatca accagctcgc tgacgtttgg cagcccggcg ttaacggatg cttcttcccg
     5521 gcaccatgca acaaactgcc cgggtgatgg cagaaatggt ctattctgcc gacgggcaac
     5581 acgcattccg gcgctcacct gttccatcgt ggtgatcccg ttttcccgga aagccagaac
     5641 ccactggcgg cggatttcgt tcagttcgtt ctggtcacga ttagccaggc tcgccgggaa
     5701 agttgccagc aactggctga atacaccgtt gattatctgc gctacctgct gtacctgcgg
     5761 cttttcgtcg tactgttccg gcatgttgtt ggcgatccga cgcatctgct cacggtcaaa
     5821 gttaatcatc tgtgcggcga tgtttttcat agatccaccc cgtaaatcca gtctgtgttt
     5881 gtcaggtcga gttttggttt gccgactgtc acgccagcct gttgcttgtt ccggttgatt
     5941 tcgagctggg tccacttgtc gcggagtttg gccggactca gcacgttacc ggaccagaag
     6001 ttgtcctggc aggcccagcg gaacagtaca cacatgtcgc ggtggttacg tccatcacgt
     6061 tcacgcatca gacggatatc gttagcccac cctgcaaaat tcggttttct ggctgatggc
     6121 gcgatggtct tcaccatgtc aaacatccac tctgcggcgg tcaggtcttc tgctgtcccc
     6181 cacttgctgc cgctctgaat cgcagcatcc gctttcacca caggaaggtc gttttctggc
     6241 aggtcagagg attcgccaga attctcggac gaataaggtt ttatattgtc ttttgttagt
     6301 ttgtcttttg tgtttacctg attcgggtaa gtgcctttac ctgatttggg taaacttttc
     6361 ttacctgatt caggtaaatt tacctctttc aggtaaactt tatttttctt acctgattcg
     6421 ggtaatgttg accattcact gaccacatta ttaatgccga tattccgccc gctctgaata
     6481 aaaatcccac gctttaccag aacacttttt gcagcagaac acttgtgcgg caatatcccg
     6541 gtcaactcgg aaagttgctc gttgctcacc cagtccagtt ttttattaaa gccatatgtt
     6601 ttgcgcatga cagccaggaa gaccagaagc tggtgctgtg ttaatccggc cagcattaca
     6661 gcttccagca actcatttgc aatgcgcgta taaccatcat cgagatctgc cacgcgcggc
     6721 tccttttgtg ccgcatccgg cactggaaaa ttgaatatct cagcagtgtt tgccataatt
     6781 cctcccgcaa tgagtgtgtt acgatttgca cctgaaagtc ggttctgttc cagcagaccg
     6841 gctttcgcca tttctgaacc tgtcatatcg cccccagcat ggtagtaacc atcgccatca
     6901 atggaccagc cagctctggg tccacacgaa acatcgacac aataccttca ctaatttcct
     6961 tcagtttctg gtggcgtggt gcgttgagaa tgacagcctg ttttgcctca ctgagttcct
     7021 tttccatttc agccaaccta gccatgaagc tatcctgctc aaccaggtaa ccgcgatatt
     7081 ccagcggtag taccgccaga attgccgggg tcagttcacg cacgttattt cggtattttt
     7141 cagaatcgaa tttgttatcg aggaagcgga acagcttctg gcgtgtacgg ctgacatcat
     7201 cagggaaatc gatggtgccg ccgccctgct cccgatactc attcacaatg agtgtggcaa
     7261 cgacatcctg attatctaca gccgaccagg cgcggacggc atcacggatt ttttcgtggc
     7321 ctggcacctg ttttgtttga gaacgattta tcaccgcagt cgggctaaat ccgctagtct
     7381 gttggtatgg aagtggttgc ataattgact cctttagttt gaattgactg ttaagttgat
     7441 tgcttattgt taaagagcgt gaaatggaaa tttaagctgc gttcttttcg gtgtgtggaa
     7501 acaacttcgg aagatccggg cgaatctggt atgccttcac tactccacca gtagccgtaa
     7561 caatgctgcc gacatgttca ggggatacct ttgctttgtt gtgaagccac ttatagacgg
     7621 cctgctgtga aacttcgcag gcagcgccca gtttcttttg tgaaccaacg atattgatcg
     7681 ctgttttgat agctgggttc ataacaacct ccgtggttaa tttgaatcaa gattaaaact
     7741 atggttgttt ttagtcaaca accattttcg tttgatggaa taaaaccttg gttgtacatt
     7801 tggactatga aaacaacact ctcagaaaga cttaaagaag ccagattagc gcgaggcctt
     7861 acacaaaagg cgcttgggga tttggtcggg gttagccagg ctgctattca gaaaatcgaa
     7921 acagggaaag ctaatcaaac aactaaaatc gtggagatcg cgaacgcttt gggtgtgcgc
     7981 gcagaatggt tatcttctgg cgttggaaat atgtcagaca gtacagtgca accaatacaa
     8041 tcaactgtca gccattccaa atacttcaag attgacgttc ttgatataga agtcagtgct
     8101 gggccgggag tcatcaaccg tgagtttgta gaagttctac gctcggttga gtactcgttt
     8161 gacgatgctc gtcacatgtt cgatggtagg aaggcagaaa atatccgcat cattaacgtg
     8221 cgtggtgaca gcatgtcagg aacgatcgaa ccaggtgatc tgctgttcgt tgatatcacg
     8281 gttaaatctt tcgacggtga tggtatctat gcatttctgt acgacgacac cgcccatgta
     8341 aagcgcctgc aaatgatgaa ggataaactg ctggttatct ctgataacaa gagctactca
     8401 ccgtgggacc ctatcgagaa agacgagatg aaccgggtat tcatattcgg taaggttatt
     8461 gggagcatgc cgcagacgta cagaaaacac ggataatcag ccgcgtgttg atgaggcttt
     8521 tgggtaatac gctgaagaaa ataccttata aaaaataaca ttacgggaaa ggcaaaaatg
     8581 agtaataaaa cacttgttaa aaaatcaaat aactgtgtcg atgcatacgc cgacgcgttt
     8641 ggctactctt cgtttggtga cggctctcag cgcctaggtt caatttcttt ttttcataat
     8701 gtaactgaat ggcctgttga tgaaagtgac gatgttgaga acataaagta caacattgca
     8761 accattagaa tgcccgaaga attgatgctt aaattggctg acttcattcg cgaccagcat
     8821 gacaaagcta aaacaaacta atcgtaatta attatgaaac acgattatta cgaagctcca
     8881 aacctaaatg ataaaattgc aaaggcggca gaatcatttt gtttgcactc cagtgcgtct
     8941 tcccagctag caggtatatc tggaaaaaga tgggacatgc tacaatcaag tggtagcagt
     9001 gggtctggga gtgataacat gcctgaaaaa gttgcaaaac tggagtccga tgttgctcat
     9061 atcaaacgag acgttgatga acttaaaaca gacgttaaag ctatcgacaa aaatatgata
     9121 actattcttg cacgtctgga ctctataaaa gaatccttaa caaacaagcc atcaagtgat
     9181 gctgtcgata gaaagatttc agacgcaaag cttgcggtat tgcttggtgt tccagcaatc
     9241 atcgctgcag gaacaggact ttataagcta tcaatgtact ttttctttag tgcttaagaa
     9301 aatgctcgca cccttgcatt aggtaaagcg atttttcatt ccccattacc ctcgctctta
     9361 accaatcatc taaacccggc caccgtgccg ggttttcttt tgccctcccc tcatcataca
     9421 ccgttcaaaa aaaccaccac gacctcgctt cagttatcgc tatgcgatgc aagtcacaaa
     9481 ataaatccat cttaaataca accagttata tctaaaacaa ccaacaaaac aacttttgtt
     9541 gttgacgata aaacaactat agttttaaat gagttcatcg caacaacaca acgatacggc
     9601 aatcacctga ttcaccgttg cgatgaccgc ttagatccgc agcttgaatt tcagcaggct
     9661 tcggggagtg cgagggatga aacggacgcg tgaacgtcgg tgtgaccagc tgaaatcaac
     9721 tcaacatttc ataccttagt cgcttcaacg aggcggctta gttatgacaa ccggcggcca
     9781 tccaccgcct gaatacgcgc agaagtcttt atatgttcag cagcccagct tacgggcagg
     9841 agtttttatg gttcatcaac attacggaac gcagaccgtt aatcgcggtg cggtcatgcc
     9901 aggaatgctg gtcaaacgca aagatggtac ctggactgca tcagctaatt tacgcggacg
     9961 actttatctg catcgcggca ttgagcgcac ttatacccgt gacttgctcg tggaagtttt
    10021 tctcgacgga cgcggcaacg ctctgaatca ctaatcccct ttcctgtttt ccgaatcagc
    10081 ctggcattcc gcgggcgatt ttttcacagc cattttcagg agttcagcca tgaacgctta
    10141 ttacattcag gatcgtcttg aggctcagag ctgggcgcgt cactaccagc agatcgcccg
    10201 tgaagagaaa gaggcagaac tggcagacga catggaaaaa ggcctgcccc agcacctgtt
    10261 tgaatcgcta tgcatcgatc atttgcaacg ccacggggcc agcaaaaaag ccattacccg
    10321 tgcgtttgat gacgatgttg agtttcagga gcgcatggca gaacacatcc ggtacatggt
    10381 tgaaaccatt gctcaccacc aggttgatat tgattcagag gtataaaacg gatgagtaca
    10441 gcactcgcaa cgctggcagg gaagctggct gaacgtgtcg gcatggattc tgtcgaccca
    10501 caggaactga tcaccactct tcgccagacg gcatttaaag gtgatgccag cgatgcgcag
    10561 ttcatcgcat tgttgatcgt cgccaaccag tacggcctta atccgtggac gaaagaaatt
    10621 tacgccttcc ctgataagca gaacggaatt gttccggtgg tgggcgttga tggctggtcc
    10681 cgcatcatca atgaaaacca gcagtttgat ggcatggact ttgagcagga caatgaatcc
    10741 tgtacatgcc ggatttaccg caaggaccgt aatcatccga tctgcgttac cgaatggatg
    10801 gatgaatgcc gccgcgaacc attcaaaacc cgcgaaggca gagaaatcac ggggccgtgg
    10861 cagtcgcatc ccaaacggat gttacggcat aaagccatga ttcagtgtgc ccgtctggcc
    10921 ttcggatttg ctggtatcta tgacaaggat gaagccgagc gcattgtcga aaatactgca
    10981 tacactgcag aacgtcagcc agaacgcgac atcactccgg ttaacgatga aaccatgcag
    11041 gagattaaca ctctgctgat cgccctggat aaaacatggg atgacgactt attgccgctc
    11101 tgttcccaga tatttcgccg cgacattcgc gcatcgtcag aactgacaca ggccgaagca
    11161 gtgaaagctc ttggattcct gaaacagaaa gccactgagc agaaggtggc agcatgatac
    11221 cggacattat cctgcagcgt accgggatcg acgtgagagc tgtcgaacag ggggatgatg
    11281 catggcacaa attacggctc ggcgtcatca ccgcttcaga agttcacaac gtgatagcaa
    11341 agccccgctc aggaaagaag tggcctgaca tgaaaatgtc ctacttccac accctgctgg
    11401 ctgaggtttg caccggtgtg gctccggaag ttaatgctaa ggcgctggcc tggggaaaac
    11461 agtacgagaa cgacgccaga accctgtttg aattcacttc cggcgtgaat attactgaat
    11521 ccccgatcat ctatcgcgac gaaagtatgc gcaccgcctg ctctcccgat ggtttatgca
    11581 gtgacggcaa cggccttgaa ctgaaatgcc cgtttacctc ccgggatttc atgaaattcc
    11641 ggctcggtgg tttcgaggca ataaaatcgg cttacatggc ccaggtgcag tacagcatgt
    11701 gggtgacgcg aaaagatgcc tggtactttg ccaactatga cccgcgcatg aagcgtgaag
    11761 gcctgcatta tgtcgtgatt gagcggaatg aaaagtacat ggcgagtttt gacgagatgg
    11821 tgccggagtt catcgaaaaa atggacgagg cactggctga aattggtttt gtatttgggg
    11881 agcaatggcg atgacgcatc ctcacgataa tatccgggta ggcgcgatca ctttcgtcta
    11941 ctccgttaca aagcgaggct gggtatttcc cggcctttct gttatcagaa atccactgaa
    12001 agcacagcgg ctggctgaga agataaataa taaacgggag gcggtatgca caaagcatct
    12061 cctgttgagt taagaacgag tattgagatg gcacatagcc ttgctcaaat tggagtcagg
    12121 tttgtgccaa taccagtaga aacagacgaa gaatttcata cgttagccac atccctttca
    12181 caaaagctgg aaatgatggt ggcgaaagca gaagcagatg agagagacca ggtatgacaa
    12241 ccactgaatg catttttctg gcagcgggct tcatattctg tgtgcttatg cttgccgaca
    12301 tggggcttgt tcaatgacac ctcagcaaga aaacgccctt cgcagcattg cccgtcaggc
    12361 taattctgaa atcaaaaaag ccagacagca ttttccggat aaaaacgtcg atgacatttg
    12421 ccgtagcgta ctaaagaagc accgcgaaac ggtaacgctg atgggattca caccgactca
    12481 tttaagcctg gcgatcggca tgttaaacgg cgtctttaag gaacggtgaa catgaaaagc
    12541 aaaatcatca gggagctaca ggctcctttt ttattgttcg cattcatcct caagcgtatt
    12601 aaccaacaat tcagggatta atggaagatg gcagacatca ttgattcagc atcagaaatc
    12661 gaagaattac agcgcaatac agcaataaaa atgcgtcgtc agaactacca gactgtatcc
    12721 gcaactcatt gttgtgagtg tggcgatccg atagatgagc gaagacgcct ggctgttcag
    12781 ggttgtcgga cttgtgcaag ttgccaggag gagatcgaac ttaagaacaa acaatgggga
    12841 ctgtgatggc ctcaaagcag caaatttcaa catcgtccaa ctgaggtgta aaaatgttca
    12901 gaatcatttt tcctaacacc tggtacgtcg accaccacgg cactccctgc aaaatcctgc
    12961 gttctaccca caacaaagtt cactacatcc gaaaaggcag aacatgtatc gccagcatgt
    13021 tccgctttaa tcatgacttt gaacctgtga ataaagctga tgcagatcgg atagcagaag
    13081 agatcgaaac ggcagaacac attaagaagt tacgtgacat gcgttcaaaa agcagaggta
    13141 accatggaat catacagcct cacactcgat gaggcctgtc agtttcttaa gatatccaga
    13201 ccaaccgcca ccaactggat acgaacaggc cgcctacagg caacacgtaa agatccaacc
    13261 aagccaaaat ctccttacct cacaacacgg caagcctgca ttgcggcgct tcagtctccg
    13321 ctgcatactg tccaggtgag cgcgggtgat ggcataacag aggaaagaaa atgtcactct
    13381 tccgcagaaa tgaaatatgg tatgcctcgt attcgctccc gggcgggaaa cgaattaagg
    13441 aatctcttgg cacaaaggac aagcggcaag ctcaggagtt gcacgacaag cgaaaagcag
    13501 aactctggcg agtagaaaag ctaggggatt tacctgatgt cacttttgaa gaggcctgcc
    13561 taagatggct tgaggaaaaa gctgataaaa aatctctcga ttcagataaa agccggattg
    13621 agttctggct tgaacatttt gagggtataa ggcttaaaga tatctcggag gcaaagattt
    13681 actctgctgt aagcagaatg cataacagaa agacgaaaga aatatggaaa cagaaagttc
    13741 aggccgccat caggaaaggt aaagaaccgc ctgtttatga accaaagcca gtatcaactc
    13801 agacaaaggc aaagcatctt gccatgataa aggccattct ccgtgctgca gaacgcgact
    13861 ggaagtggct ggaaaaagcg cctgtcatca agataccagc ggtcagaaac aagcgagtca
    13921 gatggctgga aaaggaggaa gcaaaacgcc ttattgatga gtgccccgaa ccactgaaat
    13981 ctgtcgtcaa gtttgcgctg gcaactggtc tgagaaagtc gaacatcata aatctggaat
    14041 ggcaacaaat cgacatgcag cgacgagttg cctgggtgaa tccagaagag agcaaatcaa
    14101 accgcgccat tggtgtggcg ctgaacgata ccgcctgtaa agtgttgcgt gatcaaatag
    14161 gcaagcatca caaatgggtg tttgtacata ccaaggcggc taagcgagca gatggaacat
    14221 caacgcctgc ggtcaggaag atgcgcatcg acagcaagac atcatggcta tcagcttgtc
    14281 gtcgtgcagg aattgaagat ttccgtttcc atgacctcag acacacctgg gcaagctggc
    14341 tgattcagtc aggcgtccca ttatcagtgc ttcaggaaat gggcggatgg gagtccatag
    14401 aaatggttcg taggtatgct caccttgcgc ctaatcattt gacagagcat gcgaggaaaa
    14461 tagacgacat ttttggtgat aatgtcccaa atatgtccca ctctggaatt atggaggata
    14521 taaagaaggc gtaactgatt gaattgtaat ggcgcgccct gcaggattcg aacctgcggc
    14581 ccacgactta gaaggtcgtt gctctatcca actgagctaa gggcgcgttg ataccgcaat
    14641 gcggtgtaat cgcgtgaatt atacggtcaa cccttgctga gtcaatggct tttgatctgg
    14701 ttgctgaaca agtgaacgac cgcgtctgat tttctgattt atttcgctat agcggcaaac
    14761 aaacacacac cgctgcgcgt ctgaatcaag aaaacccgta ttttcatgta tcaaagtaca
    14821 atttaccgcc ctaacggaaa attgtccgct cctatgagac tggtaactat gaaaccaacg
    14881 tcggtgatca ttatggatac tcatcctatc atcagaatgt ctattgaagt tctgttgcaa
    14941 aaaaacagtg aattgcagat tgtcctgaaa acggatgatt accgcataac catcgattat
    15001 ctccgaaccc gtcctgttga tttaatcatt atggatatag acttgcccgg aacagacggt
    15061 tttaccttcc tgaaaaggat caaacaaatc cagagcacag tgaaagtgtt atttttatca
    15121 tcgaaatcag aatgctttta tgctggcaga gcgatacaag ctggtgctaa cggttttgtc
    15181 agtaaatgca atgatcagaa tgatattttt catgccgttc agatgatcct ctccggatac
    15241 acgttttttc ccagcgaaac gcttaactat ataaaaagca ataaatgtag tacgaatagt
    15301 tcaacggtca ctgtgctatc taatcgtgaa gtgaccatat tacgttatct ggttagcgga
    15361 ttatctaata aagaaattgc cgataagtta ttacttagca ataaaacagt tagtgcgcat
    15421 aaatctaata tttatggcaa gctaggtttg cattcaattg tagagc
//
LOCUS       DAZGJW010000110        15395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZGJW010000110 DAZGJW010000000
VERSION     DAZGJW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.110"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(247..1158)
                     /gene="gsiD"
                     /locus_tag="ACHKIK_003519"
     CDS             complement(247..1158)
                     /gene="gsiD"
                     /locus_tag="ACHKIK_003519"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO9084621.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1161..2081)
                     /gene="gsiC"
                     /locus_tag="ACHKIK_003520"
     CDS             complement(1161..2081)
                     /gene="gsiC"
                     /locus_tag="ACHKIK_003520"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO9084622.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2099..3637)
                     /gene="gsiB"
                     /locus_tag="ACHKIK_003521"
     CDS             complement(2099..3637)
                     /gene="gsiB"
                     /locus_tag="ACHKIK_003521"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO9084623.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYIVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3657..5528)
                     /gene="gsiA"
                     /locus_tag="ACHKIK_003522"
     CDS             complement(3657..5528)
                     /gene="gsiA"
                     /locus_tag="ACHKIK_003522"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO9084624.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5515..6480)
                     /gene="iaaA"
                     /locus_tag="ACHKIK_003523"
     CDS             complement(5515..6480)
                     /gene="iaaA"
                     /locus_tag="ACHKIK_003523"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO9084625.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6684..7919
                     /gene="moeA"
                     /locus_tag="ACHKIK_003524"
     CDS             6684..7919
                     /gene="moeA"
                     /locus_tag="ACHKIK_003524"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO9084626.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRHGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            7919..8668
                     /gene="moeB"
                     /locus_tag="ACHKIK_003525"
     CDS             7919..8668
                     /gene="moeB"
                     /locus_tag="ACHKIK_003525"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO9084627.1"
                     /translation="MTELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8744..9406)
                     /gene="fsa"
                     /locus_tag="ACHKIK_003526"
     CDS             complement(8744..9406)
                     /gene="fsa"
                     /locus_tag="ACHKIK_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO9084628.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGNKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            9537..10436
                     /gene="ybiY"
                     /locus_tag="ACHKIK_003527"
     CDS             9537..10436
                     /gene="ybiY"
                     /locus_tag="ACHKIK_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO9084629.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10442..12874
                     /gene="ybiW"
                     /locus_tag="ACHKIK_003528"
     CDS             10442..12874
                     /gene="ybiW"
                     /locus_tag="ACHKIK_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO9084630.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13020..13835
                     /gene="ybiV"
                     /locus_tag="ACHKIK_003529"
     CDS             13020..13835
                     /gene="ybiV"
                     /locus_tag="ACHKIK_003529"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO9084631.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            13987..15252
                     /gene="ybiU"
                     /locus_tag="ACHKIK_003530"
     CDS             13987..15252
                     /gene="ybiU"
                     /locus_tag="ACHKIK_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO9084632.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVISPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         3729 a         4253 c         3918 g         3495 t
ORIGIN      
        1 aaatgacagt gctgccaata aaaataaaat tatcagcctt atctttatct tttcgtataa
       61 actcagcatg ttaatacatt taacaaaata actatctgaa cgctgtgccg ctgcaaaaat
      121 agtcccttgc tgcggctttt taaccaaatt acccgattct acaaaatcag cagaaagtta
      181 caacacttgc ttagggcaat aatttcctgt gaggtgatta ccctttcaag caatattcaa
      241 acgtaactat cctttaattt tcggatccag cgcatcgcgt aaaccatcgc ccaacaaatt
      301 gaacgccagt acggtcagaa aaatagccag ggccggaaaa acagcgacat gcggcgcgat
      361 aaccatatcc gctcgtgcct cattgagcat tgctccccac tctggtgtcg gcggctgcgc
      421 accgaggccg agaaatgaca ggctggcggc agagataatc gaggtgccaa tgcgcatggt
      481 gaaaaacacc acgatagacg agacggtccc aggcaagata tgacgcaata aaatggtcat
      541 atcgctggca ccaatactgc gtgctgactc aataaaggtt tgctgtttca acaccagcgt
      601 gttgccgcgc accagtcggg caaacgcggg gatggaaaaa atggcgactg cgataatcac
      661 gttagcaatg ccgcttccca acaccgcaac aacagcgatc gccagtaaaa tacccgggaa
      721 ggcaaacagc acatcgcaaa tgcgcatgat caaccgatcc caccagcctt cataatatcc
      781 agccaacaag cccagcaacg tcccgatcgc cgcaccgata aacacggcaa acacgcccgc
      841 cgccagcgag atttgcgcac caaccaggac acggctgaaa atgtcgcgcc ccagtgaatc
      901 gacgccaaac cagtgctgca aagaaggtcc gttattcaga ttgtcataat caaaataatt
      961 ttcggcgtca tagggagcga tccagcgtgc aaaaatggcc accacaatca ataaaataac
     1021 gaataatgcg gcggtcatcg ccatatgctg acggcgaaat cgtcgccaga attcatgcca
     1081 cggtgtacgc acctggtcag gtttgaccag tggcatggcg tttaacaccg cctgacgtcg
     1141 ccagttaaat agtcgcatcc ttacttgtac ctgatagccg ggttaatggc ggcgtaaagc
     1201 acatccacca ctaagttgat aagaataaat tccagcgaga aaagcagaat ttccgcctga
     1261 atcaccgggt aatcgcgcat ttctacggag tcaacgagta agcgtccaag tccgggccag
     1321 ttgaaaactt tctccaccac gatagaacca ccgagcaaaa agccaaactg taagcccatc
     1381 atggtcacta ccgggatcat cgcgttacgt agcccgtgtt tgaggacaac ccaggtttcg
     1441 ctcacccctt tcgccctcgc ggtacgcata taatcttcgc ttaaaacatc gacaaacgac
     1501 gcgcgggtaa agcgcgccat cacggctgcc actgccgcgc cgagcgtcag ggagggtaaa
     1561 atgtagtgct gccaactgtc tgctcccacg gtaggcagcc agcccagttc aacggagaat
     1621 acctgaatta aaagcatccc cagcgcaaat gccggaaacg agatccccga caccgcaatg
     1681 gtcatactca atcgatccgg ccaacgatta cgccagacgg cggcgataat tcccgccgcc
     1741 ataccaaata tcaccgccca gaccatactg gttatggtca gccacagcgt tggcataaag
     1801 cggctggcaa tctcatcggc aaccggacga cgcgacacca tcgacaggcc aaaatccccc
     1861 tgcacagcat tgctgatata gtgccagaac tggtgataca gcggctgatc caaccccagc
     1921 tgctgacgca ccagttctat aacctgcgca tcagcttcgg gcccggcaat caatcgcgcc
     1981 ggatcgccgg gcagcatatg cacaaataaa aacaccagca ccgagacgat aaacagcgtc
     2041 ggaatcaacc ccagtaagcg tttgataacg taattaagca ttccactccc tgcgttgctt
     2101 attgcaaatc cgcgtcttca aagctgaagc cggtgtctgg catgatccaa aaaccggtca
     2161 ggtttttact gtgtgccgac accagttttt ctaccaccag cgggatccac ggcgactctt
     2221 gccagatgat atcctgcgcc gccttataca agcgggtctt ttccgccgga tcattagttt
     2281 tcagtgcctg agccaggaag tcgtccacct gtttattgct gtaaaatgcg gtattaaaca
     2341 gcgtcggtgg ccagttttgc gaggcgaaca gtggcgatag cgcccagtcc gcttcgccgg
     2401 ttgaagccga ccagccagtg tagaacatcc gcacgccgct ctctttttgc cctttacctt
     2461 caacttctgc cgcccgctgt ccggcatcca tcgcggtcac ctgggcttta atcccgacct
     2521 gcgctaactg ctgctgggta aattgcagca ctttctgcgc ggtgctgtgg ttatgtgacg
     2581 accacagcgt ggtactgaaa ccgttgggat atcccgcctc tttcagtaat tcgcgcgctt
     2641 tcactggatc gtaaggccac ggtttataac tttgcgcgta ggcgatactt ggcggtacca
     2701 caccagtagc tggcgttgca tagcccgcaa aggcaacttt caccagcgcc ggacggttaa
     2761 tggcgtaatt cagcgcctca cggaccttcg ggttatcgaa cggcttttgc gtcacgttca
     2821 tactgatata acgctgcata attgacggac tggccatcaa ctcgatattt ttgtttttct
     2881 ccagcagtgt ggcctgctcg tagggaatgg ggaaagcaaa ctgcgcttca ccggtttgca
     2941 gcattgccgc gcgggtgttg ttatccgcca ccggacgcca ggttatgctg tccagtttgg
     3001 gcaatcctgg ctgccagtaa cccgcgaatt ttttcacctt cacaaaatcg gtctgattcc
     3061 aggtatccag ttcatacggt ccggttccca ccggatgaaa accaatctcc ttgccatatt
     3121 tttccagcgc tgccggtgaa atcatcgcgg tcgccggatg ggcaagaata ttaataaacg
     3181 ctgagaacgg ctgtttgagg gtaatcttta ccgttgtcgg atcgatcgcc tccgttttgg
     3241 caatattctt atacaggtta tagcgtttaa gatgattcgc cggatcgctg gcccggtcca
     3301 gattcgcttt taccgccact gcgttgaaat cggtgccatc ctggaattta attccttccc
     3361 gcaatttcac gatgtaagta atgccgtcat cggaaacggt ataactctcc gccagcacgt
     3421 ttttcagttt catctcttta tccagaccga acagcccctg gtaaaacgat ttcgctacgg
     3481 cctgagataa cgtgtcattt gcgtcatacg gatcgagcgt ggtgaaattc gatcctaccg
     3541 ccaccaccac atctttggca gcgaatgcac aagatgccat caacgctgtc gcaatgccca
     3601 gcgccactaa cccactacgg tgtacagctc ttgccatttt attctccgcc tgaatgttat
     3661 ctacgcatga atgcgtattc tgattgtggt tgtgcgacgt aatgccccgg cccgacgcat
     3721 tgcaacgaga cggctgccac ctcttcgcca cgcagatgaa tattgctggg aagatcgtcc
     3781 gacagcagta cacgctgcgg tcgttgtcgg gacggttcag cgaccggaac tgccgccagt
     3841 aatttacgcg tataaggatg ctgcgggttt tcgaagaccg cgcgccgtgg accaatttca
     3901 acaatttgcc cgagatacat caccgccaca cgatgactaa tccgctctac cacggccata
     3961 tcgtgggaga taaacagata cgcaatgccg aaatcacgct ggagatcgag caacaagttg
     4021 ataatctgcc cgcgaataga aacatccagc gccgaaacgg cttcgtcggc aatgatcact
     4081 tttggattca atgccaacgc gcgagcaatg cagatgcgct ggcgctgacc gccggaaaac
     4141 tcatgcgggt aacgccaggc atgttcaggt aacaggccca cgcgctccag caaccacgca
     4201 acgcgtgcag ccgcgtcttt acctggcaat aaaccgtgta cacgcagcgg ttcgataatc
     4261 gaatcaccga tggtctgacg tgggtccagc gaagcgtaag ggtcctgaaa aataaactga
     4321 atatcccggc gcaatgcctg aagtttgccg ggtgacaagg tatcgattct ctgaccgtta
     4381 aagataattt cgccgccctg cgattcgacc aggcgcagca acgcccgccc ggtagtggat
     4441 ttaccgctgc cagactcgcc caccagcgat agcgtttcgc caggccagag atcaaaacta
     4501 actttctcaa cggcatgcac ttcccgcgtt acgcgattca acaaaccgct gcgcaaaggg
     4561 aaacgggtga caagattacg cactcgtaaa acaggttcgc catccaccac cgttttctgc
     4621 tcgatggggg gggcctgttt cgctggatgt tcaagcgata tcaacgggaa acgtcgggga
     4681 taatctaacc ctttcatcgc accaagttgc ggaacagcag ctaacagcgc acgggtgtaa
     4741 ggatgttgcg gtgcatgaaa aatctgttcg acggtacccg tttccaccgc ctcgccctga
     4801 tacatcacca gtacccgatc ggcaatctct gccaccacgc ccatatcgtg agtgataaag
     4861 ataacgccca tcgacatctc tttttgcaat actttgatta attgcaggat ctgcgcctga
     4921 atagtgacat ccagcgcggt ggttggctca tcggcaatca gcaccgccgg gcggcatgac
     4981 agcgccatcg caatcatcac tcgctggcgc atcccgccag agagttgatg cggataacgt
     5041 gaaagaatgg tttgtgcctc aggaatgcgt acctgatcca gcatccgctt cgcctcgacc
     5101 atcgcttctt cacgactggc gttctgatgc agacgaattg attcggcaat ctgttcaccc
     5161 acagtaaata ccgggttcag cgatgtcatc ggctcctgaa atatcatcgc catatccgca
     5221 ccgcgaacat ggcgcatttg tgcagcgctc tgctcgctaa gttcaatcac ttcgcgactg
     5281 cgccgctgca acagcatttt atcgcactgt actaaaccgc ccgcctgttc caacaggcgc
     5341 atcaatgcca acgcagtcac tgacttaccg gagccggatt cgccaacaat tgccagcgtc
     5401 tcaccgcgtt gcagactaaa agagagattg cggaccgcag ctattttctg ctggtcctgc
     5461 ataaaggcaa tattcagatt ttcaaccgcc agcacattac cggcatcaag ttcatcactg
     5521 tgtggcaacg gtgtcccctt tttcacggta gataccggtg gttggcgtat cgcctgcgta
     5581 gccccaggcg cgatacattc cttcggtgtt aaacggtagc gcgacattcc cttcatggtc
     5641 gatagcgatt aagccaccgc taccgccaag cgcagggagt ttttccatta ctacccgctc
     5701 gcaggcttcc gcgagactta atccgccgta atccattaac gcggcgatgt catatgccgc
     5761 cagcgcgcgg atgaagactt cgcccgtgcc ggtacaagaa accgccacac tggcgttatt
     5821 ggcgtagcat ccggcaccca ctaagggact atcgccaact cgtccgggta atttattggt
     5881 cattccgcct gtggacgtgg ctgccgccaa attgccgtct aaatccaacg ccacggcccc
     5941 cacggtgccc attttctgtt tttcatccag tggcgcaccg ctatggtcga ggacggttgc
     6001 cccttccttg cgcgctgcca gtagttgttc ataacgcaaa ggcgtggaga aaatctccgg
     6061 cgagacgcgc tccatgccac gagcaaacgc aaaattttct gccccttcgc caatcatcat
     6121 cacatgcggg ctttgctcca tcaccagccg ggcggcaaga accggattac gcagatgact
     6181 aacgcccgcc accgcaccgg ctttcagggt gttaccatcc atcacacagg cgtccagttc
     6241 atgggtttca tcacgcgtaa agacagcgcc aattccggcg ttaaacagtg gacactcttc
     6301 cagcagacgc accgcttccg tcaccacatc cagcgcactt tcgcccgctt ccaacatttt
     6361 ctgcccggtt tcaacaatgg cagacaacgc ctcaatgtag cgtaattcct gttgcagact
     6421 catctgcgcg cggctaattg cacctgcgcc accatgaatt gcaatgactg ctttgcccat
     6481 agtattcgtc cattaagggt ggctcgccac ttttgctata aatatcagaa tttttatgaa
     6541 ttactcattc aatttttgaa tatagaaaga tggatatggc atgtaaaggc aggagccaac
     6601 gctacgcccg aggtggcata cagtctgtgg cgtttttctg acataatagg caaattcgat
     6661 tttgcctccg caggagtgtt ttcatggaat ttaccaccgg attgatgtcg ctcgacaccg
     6721 cgcttaatga gatgctttct cgcgtcaccc cactgaccgc ccaggaaacg ctgccactgg
     6781 tacagtgttt tggtcgtatt ctggcgagcg atgtcgtttc gccgcttgat gttccggggt
     6841 ttgataactc cgcaatggac ggctacgcgg tgcgtttagc cgatattgcc tccaggcaac
     6901 cgctgcccgt tgccggtaaa tcctttgccg gtcagccgta tcatggtgaa tggcctgcgg
     6961 gcacctgcat tcgtattatg accggtgcgc cggtgccgga aggctgcgaa gcggtggtga
     7021 tgcaggagca gactgaacaa acggacaatg gcgtgcgttt tactgctgaa gtgcgtagcg
     7081 ggcaaaatat tcgccgtcac ggtgaagata tctctgcagg tgcggttgtt ttcccggcgg
     7141 gaactcgcct gactaccgca gagctgccag taattgcttc actggggatt gccgaagttc
     7201 cggtgattcg taaagtgcgt gtagcgcttt tttctaccgg tgatgaactc cagttgcccg
     7261 gtcagccgct gggcgacggc caaatctacg acaccaaccg tctcgccgta cacctgatgt
     7321 tagaacagct gggttgcgag gtaatcaact tagggattat ccgcgacgat ccccatgccc
     7381 tgcgcgccgc atttattgaa gccgacagcc aggcggatgt agtgatcagt tccggcggtg
     7441 tttcagtggg tgaagcggat tacaccaaaa cgattcttga agagctgggg gagatcgcct
     7501 tctggaagct ggcgattaaa ccaggtaaac cgttcgcgtt cggtaaactc agcaatagct
     7561 ggttctgcgg cctgccgggc aacccggttt cagcgacgct gaccttctat caactggtac
     7621 agcctttgct ggcaaaacta agcggcaaca ccgccagcgg cctgcccgcg cgccagcgcg
     7681 tacgcacagc gtcccgcctg aaaaaaacgc caggacgtct tgatttccag cgcggcgtgc
     7741 tgcaacgcaa cgccgacggt gaacttgaag tgacgaccac cggacatcag ggttcacata
     7801 tatttagctc ctttagtctc ggcaactgct ttatcgtgct ggaacgcgat cgcggcaatg
     7861 tggaagtggg cgaatgggtg gaagtagaac cgtttaacgc gttgttcgga ggcctgtaat
     7921 gacggaactc agcgatcagg agatgctgcg ctacaaccgg caaatcattc tgcgcggttt
     7981 tgattttgac ggtcaggagg cgctaaaaga ttctcgtgtg ctggtagtcg gcctcggtgg
     8041 actcggctgc gcagcttcgc agtatctggc aagcgccggt gtcggcaacc tgacgctgct
     8101 cgacttcgac tcggtttcgc tctcgaatct gcaacgccag acgctgcaca gtgatgctac
     8161 ggtcgggcaa ccgaaggtag aatccgcccg tgacgccatg acgcggatca acccacatat
     8221 cgcgattacg ccagtcaatg cactgcttga tgacgctgaa cttgctgcga tgattgcaaa
     8281 acacgatctg gtactcgact gtacggataa cgttgcggtg cgtaatcaac tgaacgctgg
     8341 ctgttttgcc gcgaaggtgc cgctggtttc cggcgcggca attcgtatgg aagggcaaat
     8401 cacggtcttc acttatcagg acggcgaacc gtgctatcgc tgccttagcc gtttgtttgg
     8461 cgagaacgca ttaacctgcg tagaagcagg cgtgatggca ccgttgatcg gcgtaattgg
     8521 ttcgttgcag gcaatggaag cgatcaaact gctggcaggt tatggcaaac cggccagcgg
     8581 gaaaatcgtc atgtacgatg caatgacctg tcagtttcgc gaaatgaaac tgatgcgcaa
     8641 tccggggtgt gaggtgtgcg ggcagtaatt gccacctcca ggccagataa gacgcggcac
     8701 tccgccacgt catcagcaat ttatgaggat gcagaacgtc cggttaaatc gacgttctgc
     8761 caaacgctcc cagccagtcc tgttcaaact tcgccacagc ggcttccacc gccggatagc
     8821 taatcatctg ttgtgccaca tccagtggca gcgtaattga ttcacatcct gccagtaagc
     8881 agtccagcgc ctgacgcggg gttttgaaac tcgctgccag cactttcgcc tgcggcgcat
     8941 gcattttcaa taactggtgt aagtcggtca cagtctgaat gccgctacca ccctgagcat
     9001 caatacgatt aacgtaaggc gcaacatatt ccgcacctgc cagcgccgac agcagccctt
     9061 gtgctgcgcc atataccgcg gttcccagcg ttggaatccc ttccgctttt aacatcttaa
     9121 tagctgccag cccctcggcg gtcaccggaa ctttcaccac gatatccgca ataatagaac
     9181 gcaatttacg tgcgtcattc accatccctt cagcagtggt agccattacc tgggcaaaca
     9241 gacgcccctg accgcccatc gcttcatgaa gttgcggaag cacaacatcc agcggtttgt
     9301 tacccgcggc gataatgctt gggttagtgg tcacacccgc cagcggaaaa atacgtgaca
     9361 gcgccttcac cgcaacaacg tctgaagtat ccagatacag ttccataacc atcctcaaaa
     9421 tgtacttttt gtctgaatgt agactatcac gggaaaactc tctgaacagt tgacgcacat
     9481 caatctaact ttcattcgaa agcattttaa tgtttgaacg aaacagagag gcaactatga
     9541 ttttcaatat tcagcgctac tcgacccatg atggccccgg tatccgcacg gtcgtatttc
     9601 ttaaaggctg ttcgctgggc tgccgctggt gtcagaaccc ggaaagccgc gcccgcacgc
     9661 aggatctgct gtatgacgca cgactgtgtc tggaaggctg cgagctgtgc gctaaggccg
     9721 cgccggaagt gattgagcgc gcgctgaatg gtttgcttat tcatcgggaa aagttaaccc
     9781 cggagcatct gacggcgtta accgactgct gtccgacaca ggcattaacc gtgtgtggtg
     9841 aagtgaaaag cgttgaggag atcatgacga ccgttctgcg cgataaaccg ttttatgatc
     9901 gcagcggcgg cggtttaacg ctttcgggtg gtgagccctt tatgcagccg gaaatggcga
     9961 tggcgctact gcaagccagc cacgaggcag gcattcatac tgcggtagaa acctgtctgc
    10021 atgtgccgtg gaaatatatc gccccttctc tgccctatat cgatctgttt cttgccgatt
    10081 taaaacacgt tgccgacgcg ccgtttaaac agtggaccga cggtaacgcc gccagagtgc
    10141 tggataacct gaaaaaactc gccgcagcgg gcaaaaaaat cattatccgc gtgccgctga
    10201 ttcagggctt taatgccgac gaaacctctg taaaagccat taccgatttt gccgccgacg
    10261 agctgcacgt tggcgaaatt cattttctgc cctaccacac gctgggcatc aacaaatatc
    10321 acttacttaa tctgccctat gacgccccgg aaaaaccgct tgatgcgcca gaactgctcg
    10381 actttgccca gcagtatgcc tgccagaaag ggttaaccgc gaccttacga ggataacaac
    10441 catgaccaca ctgaaactgg acacgctcag cgaccgcatt aaagcgcaca aaaatgcgct
    10501 ggtgcatatt gtgaaaccgc cagtctgtac cgagcgcgcg cagcactata ccgagatgta
    10561 tcaacaacat ctcgataagc cgatcccggt acgtcgcgcg ctggcactgg cgcatcacct
    10621 ggcgaatcgc accatctgga tcaaacacga tgagttgatc attggcaacc aggcaagcga
    10681 agttcgcgcc gcgccgatct tcccggaata tactgtctcg tggatcgaaa aagagattga
    10741 tgatctggca gatcgtcccg gtgctggctt tgcggtgagc gaagagaaca aacgcgttct
    10801 gcatgaagtg tgcccgtggt ggcgcggtca gaccgtacag gatcgctgct acggcatgtt
    10861 taccgatgag caaaaaggtc tgctggcgac cggaatcatt aaagcggaag gcaatatgac
    10921 ctccggcgat gcgcacctgg cggtgaattt cccgctgctg ctggaaaaag gtcttgatgg
    10981 tctgcgtgag aaagtagcgg aacgtcgctc gcgcatcaat ctgacggtgc tggaagattt
    11041 acatggcgaa caattcctga aagcgattga tatcgtgctg gtggcagtca gtgaacacat
    11101 tgaacgtttc gctgccctgg cgcgtgaaat ggctgctacc gaaactcgcg aaagccgtcg
    11161 cgatgaactg ctgacgatgg cagaaaactg cgatcttatc gcccaccagc cgccgcagac
    11221 tttctggcag gcgctgcaac tgtgttactt catccaattg attttgcaga tcgaatctaa
    11281 cggtcactcg gtatcgtttg gtcgtatgga ccagtatctc tacccgtact atcgccgcga
    11341 cgttgaactc aaccagacgc tggatcgcga acacgccatc gagatgctgc atagctgctg
    11401 gctgaaactg ctggaagtga acaagatccg ctccggctca cactcaaaag cctctgcggg
    11461 aagtccgctg tatcagaacg ttaccattgg cggacaaaat ctggttgatg gtcaaccaat
    11521 ggatgcagtg aatcccctct cttacgcgat cctcgaatcc tgcggtcgcc tgcgttccac
    11581 tcagcctaac ctcagcgtgc gttaccatgc aggaatgagt aatgatttcc tcgacgcctg
    11641 cgtacaggtg atccgttgcg gcttcgggat gccggcgttc aacaacgacg aaatcgtgat
    11701 cccggaattt attaaactcg gtattgaacc gcaggacgct tacgactacg cagcgattgg
    11761 ttgtatcgaa accgccgtcg gtggcaaatg gggctatcgc tgtaccggca tgagctttat
    11821 caacttcgcc cgcgtgatgc tggcggcgct ggaaggcggg cgtgatgcca ccagcggcaa
    11881 agtgttcctg ccacaagaaa aagcgttgtc ggcaggcaac tttaacaatt tcgatgaagt
    11941 aatggacgcg tgggatacgc aaatccgtta ttacacgcgt aaatcaatcg aaatcgaata
    12001 tgtcgtcgac accatgctgg aagagaacgt gcacgatatt ctctgctcgg cgctggtgga
    12061 tgactgtatt gagcgagcga aaagtatcaa gcaaggcggc gcgaaatatg actgggtttc
    12121 tggcctgcag gttggcattg ccaacctcgg caacagcctg gcggcagtga agaaactggt
    12181 atttgaacag ggcgcgattg gtcagcaaca gctggctgcc gcactggcgg atgacttcga
    12241 tggcctgact catgagcaac tgcgtcagcg gctgattaac ggtgcgccga agtacggcaa
    12301 cgacgatgat actgtcgata cgctgctggc tcgcgcttat cagacctata tcgacgaact
    12361 gaaacagtac cataatccgc gctacggtcg tggtccggtt ggcggcaact attacgcggg
    12421 tacgtcatct atctccgcta acgtaccgtt tggcgcgcaa actatggcaa cgccggacgg
    12481 gcgtaaagcc cacaccccgc tggcagaagg cgctagtccg gcctctggta ctgaccatct
    12541 cggccctact gcggtcattg gctcggtggg taaactgcct acggcagcga ttctcggcgg
    12601 cgtgttgctc aaccagaaac tgaatccggc aacgctggag aacgaatctg acaagcagaa
    12661 gctgatgatc ctgctgcgca ccttcttcga ggtgcataaa ggctggcata ttcagtacaa
    12721 catcgtttcc cgcgaaacgc tgctggaggc gaaaaaacat ccggatcagt accgcgatct
    12781 ggtagtgcgt gtcgcgggtt attccgcctt cttcaccgcg ctctctccag acgctcagga
    12841 cgatatcatc gctcgtactg aacatatgct gtaatctctt cggcccggct gcggcgtcgc
    12901 cgggctaaat cattcacatc atcaatttca tccttacttt cattcgaaat ataatttgtg
    12961 ctctgcgtca cattgttatt acactgttac ggtttcattt tcgagccagg agcacaccta
    13021 tgagcgtaaa agttatcgtc acagacatgg acggtacttt tcttaacgac gccaaaacgt
    13081 acaaccaacc acgttttatg gcgcaatatc aggaactgaa aaagcgcggc attgagttcg
    13141 ttgtcgccag cggtaatcag tattaccagc ttatttcgtt ctttcctgag ctaaaggatg
    13201 agatctcttt tgtcgcggaa aacggcgcac tggtttacga acatggcaag caactgttcc
    13261 acggcgaact gacccgacat gaatcgcgga ttgttattgg cgagttgcta aaagataagc
    13321 aactcaattt tgtcgcctgc ggtctgcaaa gtgcatatgt cagcgaaaat gcccccgaag
    13381 catttgtcgc actgatggca aaacactacc atcgcctgaa agctgtaaaa gattatcagg
    13441 agattgacga cgtactgttc aagttttcgc tcaacctgcc agatgaacaa atcccgttag
    13501 tgatcgacaa actgcacata gcgctcgatg gcattatgaa acccgtcacc agtggttttg
    13561 gctttatcga cctgattatt cccggtctac ataaagcaaa cggtatttcg cggttactga
    13621 aacgctggga tctgtcaccg caaaatgtgg tagcgattgg cgacagcggt aacgatgcgg
    13681 agatgctgaa aatggcgcgt tattcctttg cgatgggcaa tgctgcggaa aacattaaac
    13741 aaatcgcccg ttacgctacc gatgataata atcatgaagg cgcgctgaat gtgattcagg
    13801 cggtgctgga taacacatcc ccttttaaca gctgacctct ctaccggagc cgtgcgctcc
    13861 ggttttccct tctttttaca tcctgtacat cacatcaaat cattaaactt gcattaactt
    13921 atttataact tatattgtca catgcaaatc agaatggttt cgaatgaaag ttaatgagag
    13981 gtggttatgg cttctacttt taccagcgac acattgcctg ccgatcacaa agcagctatc
    14041 cgtcagatga agcacgcgct gcgggcgcag cttggcgacg tccagcagat ctttaatcag
    14101 ctaagcgatg acattgccac gcgagtggct gaaatcaacg cactcaaagc acagggcgat
    14161 gccgtctggc cggtgctgtc ttatgccgat atcaaagcag gtcatgttac tgcagagcag
    14221 cgcgaacaga ttaaacgtcg cggttgtgcg gtgataaaag gccatttccc ccgcgaacaa
    14281 gcgctaggct gggatcagtc gatgctggac tatctggacc gcaaccgctt tgacgaggtc
    14341 tacaaaggcc ccggcgataa tttcttcggg acgctcagcg cttcacgtcc cgagatttac
    14401 cccatctact ggtcgcaggc gcaaatgcag gcccgccaga gtgaagaaat ggcgaatgcg
    14461 cagtcgtttc tcaatcgtct gtggacattt gaaagtgatg gaaagcaatg gtttaacccg
    14521 gatgtgagcg tcatctcccc tgaccgtatc cgccgccgtc cgcccggaac gacctccaaa
    14581 ggtcttggag cgcataccga ctccggggcg ctggaacgct ggctgcttcc agcgtatcag
    14641 cgcgttttcg ccaacgtctt taatggcaat ctggcgcaat atgatccctg gcatgcggca
    14701 catcgtacgg aagttgaaga gtacacggtg gacaacacca ccaaatgttc cgtgtttcgg
    14761 acattccagg gctggacagc gctctctgat atgctgcctg gtcaggggct gctgcacgtt
    14821 gtgcccattc ctgaagccat ggcgtacgta ctgttacgtc cgctgcttga tgatgtgccg
    14881 gaggatgaac tgtgcggcgt agcgcccgga agagtgttgc cggtatcaga gcaatggcat
    14941 ccactgttaa ttgaggcgtt aaccagcatt ccaaaactcg aggccggaga ctccgtctgg
    15001 tggcactgcg acgtcatcca ttccgttgcc cccgttgaaa atcaacaggg ttggggcaac
    15061 gtgatgtaca ttcctgcggc accgatgtgc gagaaaaatc ttgcctacgc gcacaaggtg
    15121 aaggccgcac tggaaaaagg cgcatcgccg ggcgacttcc cgcgcgagga ctatgaaaca
    15181 aactgggaag gacgctttac gcttgccgac ctcaacattc acggtaagcg agcgttgggc
    15241 atggatgttt gagagttaat ggaagtgaat tgatgtggga ttaagaaaat cgattcgacg
    15301 ttactcagat tgctgacaag tgcgctttct taatgcctga tgcgctacgc ttatctggcc
    15361 tacaaagtcc tgcagattca atatattaca ggtac
//
LOCUS       DAZGJW010000111        15346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZGJW010000111 DAZGJW010000000
VERSION     DAZGJW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.111"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            81..329
                     /locus_tag="ACHKIK_003531"
     CDS             81..329
                     /locus_tag="ACHKIK_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO9084633.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            307..1437
                     /locus_tag="ACHKIK_003532"
     CDS             307..1437
                     /locus_tag="ACHKIK_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO9084634.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHKIK_003533"
     CDS             complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHKIK_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO9084635.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2361..2450
                     /locus_tag="ACHKIK_003534"
                     /pseudo
     CDS             2361..2450
                     /locus_tag="ACHKIK_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2478..3221
                     /gene="yciC"
                     /locus_tag="ACHKIK_003535"
     CDS             2478..3221
                     /gene="yciC"
                     /locus_tag="ACHKIK_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO9084636.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            3251..3790
                     /gene="yciB"
                     /locus_tag="ACHKIK_003536"
     CDS             3251..3790
                     /gene="yciB"
                     /locus_tag="ACHKIK_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO9084637.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3895..4293
                     /gene="yciA"
                     /locus_tag="ACHKIK_003537"
     CDS             3895..4293
                     /gene="yciA"
                     /locus_tag="ACHKIK_003537"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO9084638.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHKIK_003538"
     CDS             complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHKIK_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO9084639.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            5276..5572
                     /gene="yciI"
                     /locus_tag="ACHKIK_003539"
     CDS             5276..5572
                     /gene="yciI"
                     /locus_tag="ACHKIK_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO9084640.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5871..7124
                     /gene="kch"
                     /locus_tag="ACHKIK_003540"
     CDS             5871..7124
                     /gene="kch"
                     /locus_tag="ACHKIK_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO9084641.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(7179..7352)
                     /gene="yciY"
                     /locus_tag="ACHKIK_003541"
     CDS             complement(7179..7352)
                     /gene="yciY"
                     /locus_tag="ACHKIK_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO9084642.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            7495..8955
                     /gene="cls"
                     /locus_tag="ACHKIK_003542"
     CDS             7495..8955
                     /gene="cls"
                     /locus_tag="ACHKIK_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO9084643.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8990..9319
                     /gene="yciU"
                     /locus_tag="ACHKIK_003543"
     CDS             8990..9319
                     /gene="yciU"
                     /locus_tag="ACHKIK_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO9084644.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(9372..10376)
                     /gene="oppF"
                     /locus_tag="ACHKIK_003544"
     CDS             complement(9372..10376)
                     /gene="oppF"
                     /locus_tag="ACHKIK_003544"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO9084645.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10373..11386)
                     /gene="oppD"
                     /locus_tag="ACHKIK_003545"
     CDS             complement(10373..11386)
                     /gene="oppD"
                     /locus_tag="ACHKIK_003545"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO9084646.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(11398..12306)
                     /gene="oppC"
                     /locus_tag="ACHKIK_003546"
     CDS             complement(11398..12306)
                     /gene="oppC"
                     /locus_tag="ACHKIK_003546"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO9084647.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(12321..13241)
                     /gene="oppB"
                     /locus_tag="ACHKIK_003547"
     CDS             complement(12321..13241)
                     /gene="oppB"
                     /locus_tag="ACHKIK_003547"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO9084648.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEEKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(13327..14958)
                     /gene="oppA"
                     /locus_tag="ACHKIK_003548"
     CDS             complement(13327..14958)
                     /gene="oppA"
                     /locus_tag="ACHKIK_003548"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO9084649.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
BASE COUNT         3920 a         3743 c         3632 g         4051 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc
       61 atgattagcg aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg
      121 aattcagtga accggtccca actccgagta cgttaagtaa atacgctaaa gccggaatga
      181 tatttcctct ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg
      241 tcggaatggt aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac
      301 tggaagatgg cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt
      361 gacaaaagaa ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac
      421 ggtctcggca gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt
      481 cttgcccggc agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtgatacc
      541 ggcggttcat caaccgttac catttttctg aataattaca gaaaaattca acaggaaaga
      601 tatgaaaacg gcgagatcaa actcaacacg ctgaaacaga aagcggcccc tctcagggta
      661 tttgatgaac gttttggcac cagaccgtta gatgccataa ccgtaaaaga tgtggtatca
      721 gtactggaag agtacaaggc cagaggacat aacagaatgg gacaaatttt caggaaagta
      781 ctgatcgatg ttttccggga agctcagcaa acgggcgatg tcccgccagg ctttaaccct
      841 gcagaatcgg caaaaaaacc gcaggtgcgg atatcaagac agcgactgac ttttgatgag
      901 tggatgatga tttataacgc agcggaaaag gatggttact ttttacagcg cggtatgctg
      961 ctggcactga tgacaggcca gcgcctttca gatatttgca aaatgcaatt ttcggatatc
     1021 cgggatggtt atcttcatgt cgaacagcaa aaaacaggaa cccggattgc catccctctg
     1081 gctctgcgtt gcgataaatt aaatctcacc ctggatgatg tggtgtcatc ctgccgcgat
     1141 tgcgttctta gtccgtggct attgcaccac catcacgcga aagggacagc taagcgcggc
     1201 gggatggtta agccagcaac attaaccgtt gcatttaaaa aagcccggga ttctgtggat
     1261 tacaactggc gtgctaatgg cacccctccc tctttccatg agcagagatc tttatcagag
     1321 cgattgttca gagagcaggg ggttgatacc aaaattttgc taggccattc gaatcaaaaa
     1381 atgaccgata tttacaacga cgcacgcggt aaggaatgga aaaaactggt catttgatga
     1441 ccagttttgc agaggggttt tgcagaggtt ttgcagagaa aattaaaaac gatatcctgc
     1501 ggagaacata aacacccacg gatccagtcg taccgagtct tttacggtag taacaccaga
     1561 tttgtactta gccgtggtat cgatatccat gtaccacact gacatgttaa ccaaccagtc
     1621 acggttaatc agataatcaa cccccacctg cccggcagct ccccaggaat ctttcagact
     1681 gagatcggaa agccccgcct ctttgccatg atcgttaaat ccattatcaa agaaggtggt
     1741 gtagttaata ccggccccaa cgtaaggacg gaatttgctg ctggcatcac caaaatacca
     1801 ctgcgccatc agtgttggcg gcagatggtg aacggttgca atatcgccgg tcgcccgggt
     1861 gccgatttta tggcggaacg gcgtcgctgc cagtaattcc acaccaatgt tgtcggtcgc
     1921 catataagta aacgtcaagc ccagttgagt gttattggtc acgctgaatc cacccagact
     1981 tcctaacgta ccaccagcac cttctgttgg acgtacggtt gcagaacctg cacgcataaa
     2041 aaattcgcct gcttcatgcg caaaggcact gccagaaaga agagttgtta ctgccaaagc
     2101 cgccactgtt aactttttca tatccgctcc gtcgttatgg ttataaaaac gtgacgaata
     2161 tacctacatt ggagtaacaa atgatcctac atagatcaca ttacatatga taattcacca
     2221 tttattgatc tggattaatt tagaagttgc attgagaatc ctggttaatg tgatttaaat
     2281 caatttttaa atattttctc tacgcaaata ttaactcgat cgccacttgc cgcggtttgc
     2341 ccacgcctga aggccagcaa ttacctccgc tttacaaaga tatgcttttc catacaagac
     2401 ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc cgctaattaa cacctgcata
     2461 aaactcaagg agagtgcatg tctatcacgg cgcagtccgt ataccgtgac accggaaatt
     2521 tcttccgtaa tcaatttatg accattctgt tggtatcgtt gctatgtgcg tttatcacag
     2581 tggtgttagg gcatgttttc tcacccagtg atgcacagct tgcgcagctc aatgacggcg
     2641 tgcccgttag cggcagtagt gggttgttcg acctggttca gaatatgtca ccggaacagc
     2701 aacaaatttt gctgcaggct tcagcggcgt ccactttttc aggattaatc ggtaacgcca
     2761 ttctcgccgg aggcgtaata ttaattatcc agctggtgtc tgcgggtcag agagtcagtg
     2821 cgctgcgagc tattggtgcc agtgcgccga tattgcccaa gttatttatt ctgatttttc
     2881 tgactaccct tttagtacag attggcatca tgctggtggt cgttccggga attatcatgg
     2941 ccattttact ggccctggca ccggtgatgc tggtacagga caaaatgggc atttttgcct
     3001 cgatgcgtag cagtatgcgg ctgacttggg cgaatatgcg tctggtggca cccgcagtac
     3061 tgagctggtt gctggcaaaa acactgttgc tgctttttgc ctcttctttt gccgcattaa
     3121 ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa cctgatttct gccatattgc
     3181 tcatctatct gttccgcctg tatatgttga ttcgccaata accttaagca tttgattacg
     3241 gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg gttgtctttt tcgcgtttta
     3301 caagatttat gacatctatg cggctactgc ggcgctgatc gtcgccacgg cgattgtgct
     3361 tatctatagc tgggttcgct ttcgtaaggt tgagaagatg gccctgatca cttttgttct
     3421 ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat gatgagttta ttaaatggaa
     3481 ggttacagtc atttatgctc tgtttgcggg agccctgtta gtcagccaat gggtgatgaa
     3541 aaagccgcta attcagcgga tgctgggtaa agaactcacg ctgccgcaac cggtatggtc
     3601 gaagctaaat ctggcctggg ctgttttctt tatcctttgc ggtctggcaa acatctacat
     3661 cgcattctgg ctgccgcaaa atatttgggt caactttaaa gtctttggcc tgaccgccct
     3721 taccttaatc ttcacattgt taagcggtat ctatatctac cgccacatgc cgcaggaaga
     3781 taaatcctaa ccagactgcc agtccttcgg ggctggcgct gtatctcctc tgttctcctc
     3841 aggaaaatca tagtagcatc gcgcctgtga ttttcctttt aagtcggttt taccatgtct
     3901 acaacacata acgtccctca gggcgatctt gttttacgta ctttagccat gcccgccgat
     3961 accaatgcca atggtgacat ctttggtggt tggttaatgt cacaaatgga tattggcggc
     4021 gctattctgg cgaaagaaat tgcccacggt cgcgtagtga ctgtgcgggt tgaaggaatg
     4081 actttcttac ggccggttgc ggtcggcgat gtggtgtgct gctatgcacg ctgtgtccag
     4141 aaagggacga catcggtcag cattaatatt gaagtgtggg tgaaaaaagt agcgtctgaa
     4201 ccaatcgggc aacgctataa agcgacagaa gcattattta agtatgtcgc ggttgatcct
     4261 gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa aagcctccgg tcggaggctt
     4321 ttgactttct gcttactgaa tttcggtggt gccgttaatt ttaaacagga tattcaccac
     4381 aatcccactg cctggcttac ccggctcata acgccatctg cgcatcgcat ttttcacctc
     4441 acgctcaaac atgttcgcag gcttggctga gaggatttgt acgttatcca cgcgaccatc
     4501 cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg cgcaatgcct gtgctcgtgc
     4561 cggatactgc ggctgattac ggcttaatgc gcgtggtcct gaagccacac tggtaaccgg
     4621 cttgctggtt gcagccgttg ccgtacttga tgtggggcgc gcgggtgccg tattttcaaa
     4681 cggtgatgcc ggacgcgact ctacgggttt gatatcgcgt tttggctgct cctgtacctt
     4741 tttcaccggc ttcggttttg gcttaggttt cggcttcggc ttttcaatga ccaccggtgc
     4801 ttcttttggc ggttcgggga tcggctcagg ttccggttct ggctctacca ccggctccgg
     4861 tggcggctga acggcttgtg gcggttcgag atcagcaggc gcaaccatcg tgacagaaat
     4921 tggctgcgca ggcgcaggta gttcaataac ctgatgtacc gaggtataga gcagacccgc
     4981 cacaacagca ccatgaatgc agaccgaaag taacgtcggc caggggaagc ggcgaggtaa
     5041 atcaagggtc attgaagtca taatcatttc agtagaaaaa ccaggtctcg attttaaatg
     5101 caaatagcaa tcatattcaa taaggcaaga cgatcaggat cagtgaaaat gtcatttttg
     5161 gcgtaattga ccttaattta tgcatggtct taacaaagac atcatctttc cgttgcagat
     5221 cttcgccctt tcaaataacg tactttacaa ctttcccgaa caaggagttg tgcccgtgtt
     5281 gtatgttatt tacgctcaag acaaagctga tagcctcgaa aaacgccttt ccgttcgtcc
     5341 ggcacattta gcacgtttac agttactgca tgatgaaggt cgtttgttga cagctggtcc
     5401 aatgccagcg gtagacagta acgatccagg ggctgcggga tttactggct caaccgttat
     5461 tgctgaattt gaatcgctgg aagcagctca ggcctgggcc gatgctgacc cgtacgtagc
     5521 ggcaggagtc tatgaacacg tttcagtgaa accatttaag aaagtgttct gaatagagat
     5581 tcaatattta agggcacggt ttttgcaacc gtgttcttgt ttggcctttc cttatagccg
     5641 gtcagtgcat tcaccaccac aagcagataa taactttatc tgtatttctt tatatgatgt
     5701 gaacatattc aggaaaatgc cctttcctga cgcagagttg ctcgctcaaa acctttgatt
     5761 actgccgata aagtcatcta ctattgttta acacattcac gcgctgcatg tatgttcaga
     5821 aaatgtgcac gctaagataa ttaaacaaaa ttgtggttac agaggtatca gtgagtcact
     5881 gggctacatt caaacaaaca gcaacaaatt tatgggtgac ccttcggcac gacattctcg
     5941 cgctggccgt ctttttaaat ggattgctta tttttaaaac aatctatggt atgtcggtca
     6001 atttgcttga tattttccat atcaaagcat tttcagagtt ggatctctcc ttgctggcaa
     6061 acgccccact atttatgctc ggcgtctttc ttgtcctgaa ctccattggc ttactgttcc
     6121 gggcaaagct cgcatgggca atcagtatca ttttgttgtt gatagcgcta atttacaccc
     6181 tgcattttta tccctggctg aaatttagta ttggattttg catttttacg ctggtgtttt
     6241 tgctgatact gcgcaaagac ttctcccaca gtagcgccgc agccgggaca atttttgcat
     6301 ttattagttt cacgacatta ctgttttact caacctacgg tgcgctttat ttaagcgaag
     6361 gttttaatcc gcgaatagaa agtttgatga ccgcgttcta tttttcgata gaaaccatgt
     6421 caaccgtcgg ctatggcgat attgtccctg tttctgaatc agcacgattg ttcactattt
     6481 cggtcattat ttccggcatt accgtttttg ccacatctat gacctcaatt tttggtccgc
     6541 ttatccgcgg gggattcaac aaacttgtaa aaggaaacaa tcatacaatg catcgtaaag
     6601 atcattttat tgtttgcgga cattcgattc tcgccatcaa tacgatcctg caactgaatc
     6661 aacgcggaca aaacgtaacg gttatcagca acttgcctga agatgatatc aagcaacttg
     6721 agcaacgctt aggcgataac gctgatgtta tccccggtga cagtaatgac agttcagtat
     6781 taaagaaagc gggaatcgat cgttgccggg ccattctggc gctgagtgat aacgatgcag
     6841 ataacgcgtt tgttgtactc tcggcaaaag atatgagcag tgatgtcaaa acagttctcg
     6901 ccgtcagtga tagcaaaaac ctgaataaga ttaagatggt acatccggat atcattctct
     6961 caccgcaact gtttggcagc gaaattctgg cgcgagtttt aaatggtgaa gagattaata
     7021 atgatatgct cgtttcaatg ttgttgaact ccggtcatgg cattttcagc gataacgatg
     7081 aacaagaaac gaaagctgac agtaaagaat cagtgcaaaa atagagttta aagacgaaag
     7141 agtaaactta ttgggcaccg caatggtgcc cagtatattt agatattgta gatcgcaaac
     7201 aataacgagt tacgctgttt gtttagaatg cacttcctga tgctgtggat acgcatattt
     7261 cggcgcgatt gcccctcaag ccaacgcgat ttacgtcggc tagcctgacg ctgcatgcgc
     7321 cagcgcccca cttccgttct actccgcttc atgttaacta ctctatgcaa taacagaatg
     7381 gtcattatac ccccaccccc gacgctgacc agtgcttttc ccgcgttttt atttgccaga
     7441 tgaatccata tgcgtaaact cataacaatg cgctttcaaa aggatttcta atctatgaca
     7501 accgtttata cgttggtgag ttggttggcc attctgggat actggttgct cattgcaggc
     7561 gtaactttac gcattctaat gaaacgacgc gcagttccct ccgcgatggc ctggctgttg
     7621 attatttaca ttctgccgtt agtcggaatt attgcctatc ttgccgttgg cgagctccat
     7681 ttaggcaaac gccgcgctga gcgcgccaga gcgatgtggc cttccaccgc aaaatggctt
     7741 aacgacctta aagcctgtaa gcatatcttc gccgaagaaa atagcagtgt cgctgcgcca
     7801 ttattcaagc tttgcgagcg tcgtcagggg atcgctgggg tcaaagggaa tcagctacaa
     7861 ctgatgaccg agtcagatga tgtgatgcag gcgttaatcc gcgacatcca gctcgcgcgc
     7921 cataatattg agatggtgtt ttatatctgg cagcccggcg gcatggcgga ccaggtggct
     7981 gaatcattaa tggcggctgc acggcgtggt attcattgcc gattgatgct cgactccgcc
     8041 gggagtgtgg cttttttccg cagcccgtgg cccgagctaa tgcgtaatgc cggtattgaa
     8101 gtggtcgaag ccttaaaggt caatctgatg cgtgtgtttt tacgccgtat ggacctgcgc
     8161 caacatcgca agatgatcat gatcgataat tacatcgcgt acaccggcag catgaatatg
     8221 gtcgatcctc gctacttcaa acaagatgcg ggcgtagggc aatggattga tctgatggcg
     8281 cgtatggaag gccccatcgc caccgcgatg gggattattt attcctgcga ctgggagatt
     8341 gaaaccggaa aacgtattct gccgccacca ccagatgtca atattatgcc gtttgaacag
     8401 gccagcggtc acaccattca cacaattgct tctggccccg gctttccgga agatctcatt
     8461 caccaggcat tattgactgc ggcttattcg gcgcgtgaat atttgatcat gaccacgccc
     8521 tactttgtgc caagcgatga tttacttcat gcgatttgca cggcggcgca gcgcggggtg
     8581 gatgtcagta ttatccttcc gcgaaaaaat gactcgatgc tggtcggctg ggccagtcgc
     8641 gcattcttta cggaactgct tgctgctggg gttaaaattt atcagtttga aggcgggtta
     8701 ctgcatacca agagcgtgct ggtcgatggc gaactaagtc tggttggcac cgttaacctt
     8761 gatatgcgga gtctgtggct gaatttcgag attaccctgg caatcgacga taaaggtttt
     8821 ggtgcagacc ttgccgccgt tcaggacgat tatatttcgc gttcacgtct gctcgatgcc
     8881 cgtttatggc taaaacgtcc attatggcaa cgtgtcgccg agcgactgtt ttacttcttc
     8941 agtccgttgc tgtaaaacgt cgccaacaga ggttaaacag gtagtgacta tggatatgga
     9001 tctaaacaat cgcctgactg aagatgaaac gcttgagcag gcctacgata tttttctcga
     9061 actggctgcc gacaatctcg atccagctga tgttctgctg ttcaatcttc agtttgaaga
     9121 gcgcggcggc gcggagttat tcgatccggc ggaagactgg caggaacatg ttgatttcga
     9181 tttgaaccca gacttttttg ccgaagtggt gattggtctg gcggacagtg aagatggtga
     9241 aatcaacgat gttttcgccc gcattctgtt atgccgcgaa aaagatcaca aactttgcca
     9301 tatcatctgg cgggaataaa taatcgcttt tagcgtgaaa aagggctgac aactgtcagc
     9361 ccttattgtg tttaaagcgg atcgactttc aggcaagaaa cggcgtgtct gaaactcccc
     9421 tccagaacag gacgtgtttt ggcgcactcc ggaccggcaa tcgggcaacg ggtacggaaa
     9481 acacaaccgg aaggcgggtt gatcggcgac ggtaattccc cttccagtaa ctggatggtt
     9541 ttgttcttct ccagatccgg atcaggtatg gggactgccg acatcaatgc cctggtgtaa
     9601 ggatgtagtg gattgtggta gacctcatca taggtcccca gttctaccgc atggccgaga
     9661 tacatcacca acacacgatc ggaaatgtgt tttaccacgg ccaggtcatg agcgataaaa
     9721 attaatgaca atcccatctc acgttgcagc tgctggagca ggttgaccac ctgcgcctga
     9781 attgacacgt ccagcgccga aaccggctca tcgcagataa tcagcttcgg ttcaagaata
     9841 agagcacgag caatcccgat acgctggcac tgcccaccgg agaactcatg cggatagcgg
     9901 ttaatcaggt taggcaataa cccgactttc agcatcatcg ccttcacgcg ctcgcgaact
     9961 tcctggcgtg acattttcgg atgataagta cgcagtggtt cagcgatgat ctcgccgatg
    10021 gtcatacgcg ggtttagcga tgccaacgga tcctggaaaa tcatctgaat atcactgcga
    10081 acggcacgcc attcatcggg cttcatgccc agcaactctt tacctaacca ggcaacatga
    10141 ccgtcggtcg ccttgaccaa accgatgatg gcgcgagcaa aggtggactt accgcatccc
    10201 gattccccta ccacacctaa tgtttcccct tcatacaggc gaagagtgac accatcgacg
    10261 gctttgagcg ttttcggcgg ttgccagaac cactgtttgc catctttgat ttcaaagtgc
    10321 actttaagat cggcgatttc aaggaggact tttcttcctt cagttacagc attcataaca
    10381 gttcctccac cggtttaaag caagcacgca gacggccagg cgtaaactct tccagcggcg
    10441 gtgcgctact acaaatttcc atcgcatgtg gacaacgtgg ctggaacggg caacctttcg
    10501 gtaatcgcag caagtttggc ggattacccg ggatggtcaa cattgtttca ccttccgcat
    10561 caagacgcgg caccgcgttg agcaaaccga tagaataagg atgaacgggt tgataaaaga
    10621 catcgcgcgc attgccatat tccatcgtgc gcccggcgta cattaccagc actttgtcac
    10681 agatccccgc caccacgcca agatcgtggg tgatcataat gatggcggta ttaaattccc
    10741 gcttcagttc atttaatagc gtcatgatct gcgcctgtac ggtgacgtcc agcgcagtag
    10801 tgggttcatc cgcaatcagt agcttaggtc gacatagcaa tgccatcgca atcatgactc
    10861 gctgacgcat gccgccagaa aattcatgcg ggtacatttt catgcgttta cgcgcttccg
    10921 gcatttttac cgcatcgagc atccgcaccg actcttcaaa cgcctcagct ttgctcatgt
    10981 tcttatgcag catcagcact tccattaact gctcaccgac gcgcatatag ggattcaacg
    11041 aagtcattgg gtcctgaaaa atcattgaga tttgttcagc gcgcagttta ttgagttcat
    11101 gctctggcaa attgaggatt tcacgcccat tgaaggttgc cgatccgcca atacgtccgt
    11161 tggcagccaa caggcccatc aacgcaaatg cagtttgcga tttacccgaa ccagactcac
    11221 ctacaatacc cagcgtttct ccggcacgta gggaaaaatt caaatcattg accgccgtga
    11281 cgtcgccgtc cggggtacta aaggtgacac gcaaatcttt cacgttcagc agtgcgtcag
    11341 cctgttgttg tgcgagcggc acagttgcag tttcaattac gctcatggct gcactcctta
    11401 acgatctttc gggtcgaggg catcacgcaa gccatcgccg ataaagttga aacaaaacag
    11461 cgtcaccacg aggaatcccg ctgggaacaa caataaccat ggagagactt ccatcgagtt
    11521 cgcgccatca ctcagcaatg caccccagct gcttaacggc tcttgcgtac ccaaccccag
    11581 gaagctaagg aaagattcaa agaggatcat gctgggcacc agaagtgatg cgtagaccac
    11641 caccacaccg agtacattcg gcacaatgtg gcgaataaca atgcccgatg tcgatacacc
    11701 gccaacttgt gccgcctcaa taaactcttt gcgcttcaga ctcagggttt gcccacgcac
    11761 aatacgagcc atatccagcc aggaaaccat gcctatcgcc acgaaaatca gcaggatgtt
    11821 ttgaccgaaa aaggtcacca gcaaaatgac gaagaacatg aatgggaagg agttgaggat
    11881 ttccagcaga cgcatcatta ccgaatccac tttaccgccc agataaccgg aaagcgaacc
    11941 ataaagtgtc ccgacgacca cagccaccag tgccgcagca acaccgacca tgagtgagat
    12001 acgcccgcca atcgcaacgc gcacaagcag gtcgcgaccg gatgagtcag taccaaagta
    12061 gtgaccggac tccatatccg gggcgctgga catcatcgcc cagtcagtat cgtcataggc
    12121 aaactgggaa agcatcggtg ccaggattac aaataacgcg atcagcacca gcactatcag
    12181 actggcaacc gccgcacggt tatgcataaa acgtcgacgt gcgtcctgcc acaagctgcg
    12241 cccttcgacc tccagctttt cactgaaatt ttccagcgtc tcgctgtttt tcttacttaa
    12301 catcatcgcg agctccagta tcagtaacgg attttcgggt cgataaccgc atatagcaca
    12361 tcgacaatgg cattaaacaa aatggttaaa gcaccaacca ggatggtcag gcttaacact
    12421 aaggaatagt cacggttcaa tgcgccatta acgaacagtt gtccaatccc cggcaaacca
    12481 taaatggttt cgataaccat agaaccggta ataatgccga caaatgcggg gcccatataa
    12541 gagagcacgg gtaacagagc aggttttaat gcgtgacgta aaatgatccg ccgcataggt
    12601 aaccctttcg cccgggcagt acgaataaaa ttggagtgta atacttcaat catagagcca
    12661 cgggtaatac gcgcaatact ggcgatataa gcgagtgaca acgccaccat cggcaatatc
    12721 atgaatttaa gcgccccacc attccagcca ccgcccggca gccaatgcaa aatgatcgca
    12781 aatatcatga ctaataatgg tgcaaccaca aaactgggga taacaacccc ggtcattgcc
    12841 agccccatca cggtatagtc ccatttggtg ttttgtttta atgcggcaat aacgccagca
    12901 ctaaccccca gtattaccgc aaggaaaaat gctgcggctc ccagtttggc agaaacggga
    12961 aaactggatg caaccaagtc attgaccgaa taatctttat atttaaacga tggaccgaaa
    13021 tcaccgtgcg ccagttgttt caggtagctg aaatactgtg tcatgatcgg atcattaaga
    13081 tgatatttct cttcgatatt ggccatcact tctggcggta aagtacgttc gccggtaaaa
    13141 gggcttcccg gcgcgaggcg catcataaag aacgaaatag taataagaat aaatagcgtc
    13201 ggaatcgctt ccagacagcg acgtagaata aattttaaca ttgccctacc ttctggcctg
    13261 tgccttctgt aatgcgataa aaatcagaca ccgtggagca ggacactcct gccccacgta
    13321 ttgccattag tgcttcacaa tgtacatatt ccgggtatag gtattatcca gcggatcttt
    13381 gccggtatag ccaccaaccc acggtttcac cagacgcgca ttcacgtagt aataaacagg
    13441 aacaatggcc gaatccttat ccagctgttg ttctgcttta gtgtacagag ctgtgcgctg
    13501 cgcctcgtca gtcactttca gcgtttccgc cataatgctg tcaaaggccg ggctcttata
    13561 atgcgcggta ttcatcgagc tgttcgaaag catggtgttc aggaaggaag ttggttcgtt
    13621 gtagtcagca caccagcctg cacgggccac atcaaaagta ccctggtgac gggtgtcgag
    13681 gaacgttttc cactcctggt taaccagttt gacgtttaca ccaatgtttt tcttccacaa
    13741 tgaagaggca gcaatcgcca gctttttatg cagatcggag gtgttataca acaggttgat
    13801 ggtcaacggt ttgtctgcgg tataacccgc ttcagccagc agttttttcg cttcttcgtt
    13861 acgtttttcc tggctccagc caaaccattc cggctgagtc aattttgcgc catcagtata
    13921 cggtggagtg taaccatagg cgggcatgtc gccctgcgct ttcactttat taacaatgat
    13981 atcgcggtcc atacctagtt tcagtgcggt acgcacacgc acatcgttga atggcggttt
    14041 ctggttgtta atttcgtaat agtaagtgca caggtatgga tcaacgtgaa cttcgtccgg
    14101 gatctctttt ttcagcttct ggaacaattc gatcggcatg ttgttataag tcatgtcgat
    14161 ttcaccacta cggtagcggt tgacatcggt aacttcagaa gcaataggca aataggttac
    14221 ctggttaata acggttttcg cgttgttcca gtaggtcggg ctgcgttcaa gaacgattcg
    14281 ttcgtttacg acccaatctt ttaaggtata ggcaccgttg gtgacgatat taccaggctg
    14341 ggtccatttt tcgccgaatt tctcgatagc ggcttttggc accggtgaag ttgatgggtg
    14401 aacaagtaat ttatagaagt acggaacggg ttcacttaag gtgacttcta atgtgtgatc
    14461 atcaatagct ttcacgccga gatcggtaat cggttttttc ccttcaagaa tttcatcaat
    14521 accggcgata tgcccatatt gcagataact ggcatacgga gaagcagtgt tcggatcaac
    14581 agaacgttgc cagctataca caaagtcttg tgctgtgact ggcgtgccat cagaccattt
    14641 cgcatcttta cgcaaatgga aggtccagac tttcgcgtct ttattatccc aggattcagc
    14701 gacgccaggt gctggatgac cgtcaagatc gctgaccagt aagccttcaa acaggtctcg
    14761 gctgatatta gactccggaa caccttcaat tttgtgcgga tctaatgact gaacttctga
    14821 accattgtta cgtaccagtg tttgtttttc cgccagtgtg acgcctgcgg gtacatcagc
    14881 tgccagcgcg acattccctg ccattagcgc agccagaacg ccagctgcta ctaaacttct
    14941 cttggtgatg ttggtcattg ttttttggac tccctcatta taattactgg tattaaccag
    15001 catgtgtaat cccggattgg gaccctgcac aggctcggag actttctgct gtcaggttac
    15061 tttatcgtta cgtattacta tcaccgactt tatttattct ggtgattcag acgatcacct
    15121 tatgtcgatt cgtactgtgt ctcccctgtc gagacacatt attctttcgc gacgctcttt
    15181 aattgttaat tattctcatt accaataaat aatcgtctgg cggttgcccg gaaagtacca
    15241 aatgcggttc ttactcgcca atacattttg caaaattgtt aagcaattct cttttattga
    15301 ccgtatcaga atctatagct tgcatccatg ccttaacaaa catctt
//
LOCUS       DAZGJW010000112        15316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZGJW010000112 DAZGJW010000000
VERSION     DAZGJW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.112"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(341..1165)
                     /gene="iclR"
                     /locus_tag="ACHKIK_003549"
     CDS             complement(341..1165)
                     /gene="iclR"
                     /locus_tag="ACHKIK_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO9084650.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            1365..5048
                     /gene="metH"
                     /locus_tag="ACHKIK_003550"
     CDS             1365..5048
                     /gene="metH"
                     /locus_tag="ACHKIK_003550"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO9084651.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYCESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            5268..6899
                     /locus_tag="ACHKIK_003551"
     CDS             5268..6899
                     /locus_tag="ACHKIK_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO9084652.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(6990..7679)
                     /gene="pepE"
                     /locus_tag="ACHKIK_003552"
     CDS             complement(6990..7679)
                     /gene="pepE"
                     /locus_tag="ACHKIK_003552"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO9084653.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8062..9291)
                     /gene="sorE"
                     /locus_tag="ACHKIK_003553"
     CDS             complement(8062..9291)
                     /gene="sorE"
                     /locus_tag="ACHKIK_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO9084654.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            complement(9343..10166)
                     /locus_tag="ACHKIK_003554"
                     /pseudo
     CDS             complement(9343..10166)
                     /locus_tag="ACHKIK_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(10177..10974)
                     /locus_tag="ACHKIK_003555"
     CDS             complement(10177..10974)
                     /locus_tag="ACHKIK_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO9084655.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQPQATTSTALDQLDD"
     gene            complement(11040..11534)
                     /locus_tag="ACHKIK_003556"
     CDS             complement(11040..11534)
                     /locus_tag="ACHKIK_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO9084656.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            complement(11534..11941)
                     /locus_tag="ACHKIK_003557"
     CDS             complement(11534..11941)
                     /locus_tag="ACHKIK_003557"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO9084657.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            complement(11951..12757)
                     /locus_tag="ACHKIK_003558"
     CDS             complement(11951..12757)
                     /locus_tag="ACHKIK_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9084658.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(12827..13774)
                     /locus_tag="ACHKIK_003559"
     CDS             complement(12827..13774)
                     /locus_tag="ACHKIK_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO9084659.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELRIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            14122..14994
                     /gene="rluF"
                     /locus_tag="ACHKIK_003560"
     CDS             14122..14994
                     /gene="rluF"
                     /locus_tag="ACHKIK_003560"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO9084660.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEVKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(14995..15267)
                     /locus_tag="ACHKIK_003561"
     CDS             complement(14995..15267)
                     /locus_tag="ACHKIK_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO9084661.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
BASE COUNT         3649 a         3999 c         4022 g         3646 t
ORIGIN      
        1 actacgaggg atacgagtaa tcatttaaat atctcattgt ttattgatgt cttgaatttt
       61 aactatcaga acagtgaaaa aatttaatat gattacaact aaagaaatat cataaatcgc
      121 tcaatctcat aatgcagccg taaaagtttc ggtggaatga gatcttgcga ttttgttaat
      181 gattaagtag tttgatgttt aaaaataatg ctgagttaat aaaagtgtgt gaagtgtatg
      241 acattaagtt attttggcgt caatgcgatt aacagacacc cttattctat tgccactcag
      301 gtatgatggg cagaatattg cctctgcccg ccagaaaaag tcagcgcatt ccaccgtacg
      361 ccagcgtcac ttccttcgcc gctttaatca ccatcgcgcc aaactcggtc acgcggtcat
      421 cggtaatacg tgaaatcggt ccggaaattg aaattgcggc aaacggttcg cggtgctcat
      481 cgaaaataca cgctgcaagg caacgtagcc ccagtgcatg ttcctcatcg tcaaatgaat
      541 aaccccgttt gcgcgtttgg gcgagatctt cttttaaatg cacaggagac accagcgttg
      601 cgtgggtata ggcatgtaac cctttgcggt gcagcagctt cgtcacctgt tcttcgctca
      661 gttgggctaa aaaggcttta cccgcaccgg aagcgtgcat cggcaattta ccgccgatag
      721 gcgcggacat tcgcatcaga tgcgtacact gtacctggtc gataataatc gcttcgtgat
      781 cgctttgatc aagcaccgcc atattgaccg tttcgccaga ctcttccatt aaattgcgca
      841 ggatagggtg aacaatcgct aacaaattac ggctctggag aaagctgctg ccgaccataa
      901 aggcatgtgc gccgattgcc caatgtccca gttcgccgac ctgacgcacg aaaccctgct
      961 gttgcatcgt ggttagcagg cggtgggtcg tggaattggg taacccggct tgttgcgcca
     1021 gctctgtgag tgccacactg ccattggatt cggcaatcca ctccagtaat ttcaggccac
     1081 gcgttaaaga ctgaacctgt ccagtcgctg gtgcggtggc aacggcgggt tttctgccgc
     1141 gtttcgcggg aatgggtgcg accatgacag cctccttttt ctgtatcgtg gaaatcattt
     1201 tcatttttat tgttagctaa tgcaatagtt gctgaactga tccgatgagt taatgttgaa
     1261 caaatctcat gttgcgtggt ggtcgctttt accacagatg cgtttatgcc agtatggttt
     1321 gttgaatttt tattaaatct gggttgagcg tgtcgggagc aagtgtgagc agcaaagtgg
     1381 aacaactgcg tgcgcagtta aatgaacgta ttctggtgct ggacggcggt atgggcacca
     1441 tgatccagag ctatcgactg aacgaagccg attttcgtgg tgaacgcttt gccgactggc
     1501 catgcgatct caaaggcaac aacgacctgc tggtgctcag caaaccggaa gtgatcgccg
     1561 ctatccacaa cgcctacttt gaagcgggcg cggatatcat cgaaaccaac accttcaact
     1621 ccacgaccat tgcgatggcg gattaccaga tggaatccct gtcggcggaa atcaactttg
     1681 cggcggcgaa actggcgcgc gcctgtgctg acgagtggac cgcgcgcacg ccggagaaac
     1741 cgcgctacgt tgccggtgtt ctcggcccaa ccaaccgcac ggcgtctatt tctccggacg
     1801 tcaacgatcc ggcattccgc aacatcactt ttgaccagct ggtggcggcc tattgcgaat
     1861 ccaccaaagc gctggtggaa ggtggtgcgg atctgatcct gattgaaacc gttttcgaca
     1921 cccttaacgc caaagcggcg gtttttgcgg tgaaaacgga gtttgaagcg ctgggcgttg
     1981 agctgccgat tatgatctcc ggcaccatca ccgacgcctc cgggcgcacg ctctccgggc
     2041 agaccaccga agcattttac aactcattgc gccacgccga agctctgacc tttggcctga
     2101 actgtgcgct ggggcccgat gaactgcgcc agtacgtgca ggagctgtca cggattgcgg
     2161 aatgctacgt caccgcgcac ccgaacgccg ggctacccaa cgcctttggt gagtacgatc
     2221 tcgacgccga cacgatggca aaacagatac gtgaatgggc gcaagcgggt tttctcaata
     2281 tcgtcggcgg ctgctgtggc accacgccac aacatattgc agcgatgagt cgtgcagtag
     2341 aaggattagc gccgcgcaaa ctgccggaaa tccccgtagc ctgccgtttg tccggcctgg
     2401 agccgctgaa cattggcgaa gatagcctgt ttgtgaacgt gggtgaacgc accaacgtca
     2461 ccggttccgc taagttcaag cgcctgatca aagaagagaa atacagcgag gcactggatg
     2521 tggcccgcca gcaggtggaa aacggtgcgc agattatcga tatcaacatg gatgaaggga
     2581 tgctcgacgc cgaagcggcg atggtgcgtt ttctcaatct gattgccggt gaaccggata
     2641 tcgctcgcgt gccgattatg atcgactcct caaaatggga tgtcattgaa aaaggtctga
     2701 agtgtatcca gggcaaaggc attgttaact ctatctcgat gaaagagggc gtcgacgcct
     2761 ttatccatca cgcgaagctc ttgcgtcgtt acggtgcggc agtggtggta atggcctttg
     2821 acgaacaggg acaggccgat acccgcgcac ggaaaatcga gatttgccgt cgggcgtaca
     2881 aaatcctcac cgaagaggtt ggcttcccgc cagaagatat catcttcgac ccgaacatct
     2941 tcgcggttgc caccggcatt gaagagcaca acaactacgc gcaggacttt atcggcgcgt
     3001 gtgaagacat caaacgcgaa ctgccgcacg cgctaatttc cggcggcgta tctaacgttt
     3061 ctttctcgtt ccgtggcaac gatccggtgc gcgaagccat tcacgcagtg ttcctctact
     3121 acgctattcg caatggcatg gatatgggga tcgtcaatgc cgggcagctg gcgatttacg
     3181 acgacctgcc cgctgaactg cgcgacgcgg tggaagatgt gattcttaat cgtcgcgatg
     3241 atggcactga gcgtttactg gagcttgccg agaaatatcg cggcagcaaa accgacgaca
     3301 ccgctaacgc ccagcaggcg gagtggcgct cgtgggaagt gaataaacgt ctggaatact
     3361 cgctggtcaa aggcattacc gagtttatcg agcaggatac cgaagaagcc cgccagcagg
     3421 ctacgcgccc gattgaagtg attgaaggcc cgttgatgga cggcatgaac gtggtcggcg
     3481 atctgtttgg cgaagggaaa atgttcctgc cacaggtggt caaatcggcg cgcgtcatga
     3541 aacaggcggt ggcgtatctc gaaccgttta ttgaagccag caaagagcag ggcaaaacca
     3601 acggcaagat ggtgatcgcc accgtgaagg gcgacgtcca cgacatcggt aaaaatatcg
     3661 ttggtgtggt gctgcaatgt aacaactacg aaattgtcga tctcggcgtt atggtgcctg
     3721 cggaaaaaat tctccgtacc gctaaagaag tgaatgctga tctgattgga ctttcggggc
     3781 ttatcacgcc gtcgctggac gagatggtta acgtggcgaa agagatggag cgtcagggct
     3841 tcaccatccc gctattgatt ggaggcgcga cgacctcaaa agcgcacacg gcggtgaaaa
     3901 ttgagcagaa ctacagcggc ccgacggtgt atgtacagaa cgcatcgcgc accgttggtg
     3961 tggtggcggc gctgctttcc gatactcagc gtgatgattt tgtcgctcgt acccgcaagg
     4021 agtacgaaac cgtacgtatt cagcacgggc gtaagaaacc gcgcacacca ccggtcacgc
     4081 tggaagcggc gcgcgataac gatttcgctt ttgactggca ggcttacacg ccgccggtgg
     4141 cgcaccgtct cggcgtgcag gaagttgaag ccagcatcga aactctgcgt aattacatcg
     4201 actggacacc gttctttatg acctggtcgc tggccgggaa gtatccgcgc attctggaag
     4261 atgaagtggt gggcgttgag gcgcagcggc tgtttaaaga cgccaacgac atgctggata
     4321 aattaagcgc cgagaaaacg ctgaatccgc gtggcgtggt gggcctgttc ccggcaaacc
     4381 gtgtgggcga tgacattgaa atctaccgtg acgaaacgcg cacccatgtg atcaacgtca
     4441 gccaccatct gcgtcaacag accgaaaaaa cgggattcgc taactactgt ctcgctgact
     4501 tcgttgcgcc gaagctttct ggtaaagcag attacatcgg cgcatttgcc gtgactggcg
     4561 ggctggaaga ggacgcactg gctgatgcct ttgaagcgca gcacgatgat tacaacaaaa
     4621 tcatggtgaa agcgcttgcc gaccgtttag ccgaagcctt tgcggagtat ctccatgagc
     4681 gtgtgcgtaa agtctactgg ggctatgcgc cgaacgagaa cctcagcaac gaagaactga
     4741 tccgcgaaaa ctaccagggc atccgtccgg caccgggcta cccggcctgc ccggaacata
     4801 cggaaaaagc caccatctgg gagctgctgg aagtggaaaa acataccggc atgaaactca
     4861 cagaatcttt cgccatgtgg cccggtgcat cggtttcggg ttggtatttc agccacccgg
     4921 acagcaagta ctacgcggtg gcgcaaattc agcgcgatca ggttgaagac tatgcccgcc
     4981 gtaaaggtat gagcgtctcc gatgttgagc gctggctggc accgaatctg gggtatgacg
     5041 cggactgatt cacaaatctg tcacttttcc ttacaacaaa cagggcgctc aatgagtgcc
     5101 ctgtctcttt attaatatga aacacttata ctggaaacag gctggaataa tcttagccgg
     5161 aaaaacgaat gtcaaaaggc acagtaaaga tgccagtcag cgtgtttccg gtcctttggt
     5221 cattcaattg cttcaggaac atataacgat aaggagaacc tgaactcgtg ttaacgctgc
     5281 ttcacctgct ttctgccgtc gccctgctgg tctgggggac tcatattgtt cgaaccggcg
     5341 taatgcgcgt cttcggcgcg cgtttgcgta ctgtccttag ccggagcgtc gaaaaaaagc
     5401 cgctcgcctt ttgcgcgggg atcggcgtta ccgcactggt gcagagcagt aatgccacca
     5461 ccatgctggt gacctcgttt gtcgctcagg atctggtagc cctcgcaccg gctctggtca
     5521 ttgtgctggg ggcagatgtc gggacggcgc taatggcgcg tattctcacc ttcgacttat
     5581 cctggctgtc accgttactt atttttatcg gcgtgatttt tttcctcgga cgcaaacagt
     5641 cacgcgccgg gcaactgggc cgcgtcggta ttggtcttgg gctgatttta ctggcgctgg
     5701 agttgattgt gcaggccgta acgccgatca cccaggcaaa cggcgttcag gtgatctttg
     5761 cctcgctgac cggcgatatt ctgctggatg cgctgattgg cgcgatgttc gccattatca
     5821 gctactccag ccttgctgct gtactgctga ctgcgactct gaccgccgca ggcattatct
     5881 ccttccccgt ggcgctctgt ctggtgattg gtgctaacct cggttccggc ctgctggcga
     5941 tgctcaacaa cagtgccgcc aatgccgcag cccgccgtgt cgcgctgggt agtctgctgt
     6001 ttaagctggt gggtagcctg attatcctgc cgtttgtcca tttgctggca gagacaatgg
     6061 ggaagctgtc attgccaaaa gcggaactgg tgatctattt ccacgtcttc tacaaccttg
     6121 tacgttgcct ggtcatgctg ccatttgttg acccgatggc acggttttgc aaaacgatta
     6181 ttcgcgatga accggaactg gatacccagc tacggcctaa acatctggat gtcagcgcgc
     6241 tggatacgcc cacgcttgct ctggcgaacg ccgcgcgcga aaccctgcgc attggcgacg
     6301 ccatggaaca gatgatggaa gggctgaata aagtgatgca cggcgagcca cggcaggaga
     6361 aagagctgcg taagctggca gatgatatca acgttctcta taccgccatt aagctgtatc
     6421 tggcgcggat gccaaaagag gaactggcag aagaagagtc gcgtcgctgg gcggagatta
     6481 ttgaaatgtc gctcaacctt gaacaggctt ccgatatcgt cgagcgcatg ggcagcgaaa
     6541 ttgccgataa atcgctggca gcacggcggg cattttcgct tgatgggttg aaggaactgg
     6601 atgcgctcta tgagcaattg ctcagtaatt taaagctggc aatgtcggtg ttcttctctg
     6661 gcgatgtcac cagcgctcgt cgtttgcgtc gcagcaaaca tcgttttcgc attcttaatc
     6721 gccgctattc ccacgcccac gtcgatcgcc tgcatcagca aaacgtgcag agcattgaaa
     6781 ccagttcgct acatttaggc ttactgggag atatgcagcg cctgaactcg ctgttttgtt
     6841 cggtggctta cagtgtgctg gaacagccgg atgaagatga aggacgggac gagtattaat
     6901 attgtgaatc ctgtaggcct gataagacgc gtcaagcgtc gcatcaggca ctatgaacaa
     6961 gatgccggat catgcatccg gcaatttact taaaaacggt gaccagcttc tagcggcacc
     7021 gcttcttcac cagccttaaa cacataagtg gtgttcgggc cacccagcgt agcgtgacct
     7081 ttactcactg tgatccagtt accttccggt agaccaataa tcgtcagttc tggcgcgacg
     7141 accagcagtt cgcgaatacg ctgctcacgg gtttcacctt tatggccttc cggcagcgcg
     7201 ttggtgaagt gcgggttgat ttgcagcggg aacagattta gcgcatcgaa accttgcgga
     7261 tcgacaatcg gcatatcgtt ggtggtacga atggttgggc aagcaaggtt agcgcctgcg
     7321 ctccagccaa tatacagcgc accacgttta accacgtcag taattggtgc cagcagcccg
     7381 cgctcgcggc actgtttcag caactggaaa gtattcccgc ccccgacaat cacgatctca
     7441 gcattttcaa tcgcggcaac gggatcgaca acgctatgaa taccggtgac agatacaccc
     7501 agcggagcga gaaccgcagc cgttttcgct gtgtaatcat cccaggtctg cgttacgcca
     7561 gcgaaaggaa taaataccgc tgaacggcga ccctgcaact gttcagcaat cagcggcagc
     7621 gcatgttcca gccaggcttt acccggcagc gtcgagttac tcaataaaag cagttccatt
     7681 acttctccag ttagtaagaa atcacgggag gcatgctacc actgcgaatt taaccactta
     7741 ctgacatggc tcacgatgtc acgtattgca ccgggcgctg agactgatga caaacacagc
     7801 attgcctgat gcgctacgct taccaggcct accagataac tgcaatatat tgaattttcg
     7861 agattatgta ggccgaataa ggctttattc cgcatccggc ataaacaatg tgtactttgc
     7921 caccaatttg agcgcccctt aaggcgctga tgttttttta tccttctcgt gcacattggg
     7981 ccgcttcagc aatcagccac acatcaggtc gcgaagttgc cgcggcgacc agccagcgac
     8041 atgcagagtt aagtagcgcg attaatcatg ggaaattgcc tctgcatgag tgagcagata
     8101 ttgctccgcc tcaccggacc agatcccctg atgacgcgcc agaatcgccg ccagtgcttg
     8161 atcctgaatc tgcgtgagtg acgtcagcgg caggtatttc ccggtataca ccagcttttt
     8221 gccgccgccg acggcaggca attcaagcgt ggtttcgccc gcggcattca gcccaagaat
     8281 atgtgttacc acttttgcgg cctgcacttt tttctcttca atcagtttga ccgccgcgcg
     8341 catgtcgtcg gtattgccgc ctgacgtgcc cacgtagtgg gtaaatgcat aatgcacatc
     8401 gtagaaatta attggcgcgc tgaaatgttt atcctgcggt ccggcgaaga aattcaggca
     8461 accatctgtc gccagcaagg aagaggcgag agtcaccagt ccttcattag gcacaaagac
     8521 gaaaatatca tcgaagccgt gaccgccact cagcgccatt agcgtatcaa atgctgcatc
     8581 ggcggcattg agataatgaa tcagtgtttg cggttctgat ggatagtgct tgcgcgcata
     8641 actcaattta tcgttgtcgg tatcggtaat gacgagcagc gacgggttaa cgggtccatg
     8701 tagcgcataa tcaatcgcca acagtcccat tggtccggtg ccgccgagga tcagcatgcg
     8761 cccttgcggg cgaatcccca tcgtgtggtt ataactacct tcctgaagat gatagttggc
     8821 gttgaacgcg ccaatcacgc aggaaagcgg ttcaaccagt gagccttcaa aataggtttc
     8881 gccgtcgtat gccagcaggc aatcttgttc catgacctcg ttgggaataa ccacatgcgt
     8941 ggcttcgccg cccacccacg ggaaggagta gccggggcag tccgggcggt cggggagttg
     9001 caggttggcc tgaatcacat aacgctgacc cggctggaat ttgtgctgcc actttttacc
     9061 cacggccaga atatcgccgc aaaactcgtg gccgatgatg atggggttgg tcgccacatc
     9121 gtcgggtact tttttatggt tttcacccag attggcctct ttccaggaag agaggcacag
     9181 gctgtcagtg accaccgtcg cgaggatttc atcctcctgc atttcaggaa ggtcaaaggt
     9241 ttccaggcgt aaatcacgtt taccataaag acgcagagct gttgttttca taaaccacct
     9301 catgttgagc gatggccccg gcagtcgtgc cggggcgaag aatcaggaca ggaagcccag
     9361 cgcattaccg ataatcccta agccaaacag ggcgaaaatc agccataccg ggttaatttt
     9421 tttgttgagc agacgaacca taagcagcgt cagaccgagc gccagcaaac cagggcaaag
     9481 ttggtcgaga atgttctgca cggtcatggt gacggtggag ccatcggcgg catgcgtttg
     9541 tgaaaccacc aacggtacgt tgattgacgt ccatttggtg accagcacgc ccatcacaaa
     9601 cagtccgaga atcgacgcgc cttcggtgag tttttgcagc acattcccgc ccatatcgct
     9661 gacgatattc acccctttgc gaaagccgag ctgtaggcca taccacttca tcgccagacg
     9721 caccgcattg aaaataaaga aaaaaaaagc gggccgagga tgttgccaga aagtgccaga
     9781 gatgcgccga gcgcagcggt aatcgggcgc agcgttcccc aaaccagtgg atcgccaacc
     9841 cctgccaatg gtcccatcag accgactttg atgccgttaa tggtaccgtc atcaatttcc
     9901 gcgccgttgg cccgcgcctc ttccatggcg gcagtgacgc cgatgaccgg gccacatacg
     9961 gctggcgtgg tattgaagaa caccaggtgt cgcctgagcg ccgcaacctg atcctctttt
    10021 aatgggtaaa gtcgcttgat ggcggggatc atgtcgtagc aaaagcccag cccgtgaata
    10081 cgttcaaagt taaaggacgc ctgttgcagg ttggagcgca gaaacatgct caccagatcg
    10141 ctgcgtgtaa tttttctctg ttccatgatg gctccgttaa tcgtcaagtt ggtcaagggc
    10201 ggtggaggtg gtggcctggg gttgaggttc agctttacgc cactgtggat ttagctggat
    10261 gtagaccagg gccatgatca cgccgacgcc accgaacgcc agcagactga gatcgaggta
    10321 gccacctgcg aggaaaccga ggaaaaagaa aggcatcaaa tatttcacgc ccatcatacg
    10381 tagcaccatg gcgtaaccga ccaccacgat aaaaccgcca gcaatttgca gaccgcgcgt
    10441 cacgaattcc ggtatcgcgc tcagcatatt gctcaccata tcggcgctga cgaacagtga
    10501 gacaatcagt gccggaatag caacgcgcag cgcctgcacg ccaagcgcgg agacatgcag
    10561 aatatcgaga gtgcgaaacc gcgcttcttc cgctgcttta tccgccgcgt gctggaacac
    10621 tacggtgatg gtacgggcaa acacagtcag cacctggcct gccgcagcca caggcaacgc
    10681 gatggcgatt ccggtggcga tgctttgctg accaacgata accagaatgg cggagattat
    10741 gctggcgagt gcagaatccg gagattgcgc cgcgccgacg ttcatccagc cgagagctat
    10801 caattccagc gtaccaccga gtattattcc ggtttttaaa tcaccgagaa ttaaaccaat
    10861 caccgtacag gcaattaacg ggcgatgagt ctgaaattca tccagcacgc tgcccattcc
    10921 ggcaatacag gaaaaaagaa atatagcaat gatttgtagg gtacttattt ccatgatttt
    10981 tcacctttta agtgtcgtca tccctgcctg ttaaagcata tgtacacagg cgctattatt
    11041 taatttgcga ttaactgttc gtttattttg tcgataatat tgattgaggg atctgacgct
    11101 acgacgcgta aatccagtat gacgccaaga ttatttaact catgaaatgc gttaatatca
    11161 tcatcatcta atgaaaccgc tttggttaac tgttttttac cgggccgcca ggccatgccg
    11221 ccaatattca gggtgtctat ttttaccccc tggcgaacca tcgctaaggc atcctgtggg
    11281 cgagtgaaaa gataaaaaac ggtttcgtcc tgatattgcg ggttgtgata aacagcgacg
    11341 gctttttcaa tattgaccac gttaaccttc attcccggtg gcgcagcctg acgcaataat
    11401 gtccggcgaa cttcatcgtt ataaacttcg tcattgcaga taataatgcg ctgggcgtta
    11461 gccactttcg accagacggt agtgacctga ccgtggatca ggcggtcatc aatgcgggcg
    11521 agcgtaatgt tcatcagaaa tcctcctctg ccgttgccag ttgtttccag acaacgcagg
    11581 tttgctttgc gatttgcgtc aggtgttcac acagttcatc aacattcatg ctgtcctggt
    11641 tatcaaccag ttcgagcgcc agcggcagag aaaggccgct gattacccgc agacgaggat
    11701 tgcgcatcgc aagcatcgct gccgcattcc acgggctgcc gcactgtaaa tccacggcaa
    11761 tcagccactc atcttgattg tgaatgctta ctaacttttc cagctttgct acgatgtctc
    11821 cggcgttctc gccgggcaca aattcaacgg cttcaacatt ggcgttgccg taaaccatct
    11881 gaaccgattc cagcatggcg caagctaatt tgccgtgggc acaaaaaatg gcgttgacca
    11941 taaagcctcc ttagccgcgc gttttgccgc ccgcaatgtt agtggttact ccggtgatgt
    12001 agctggcgcg ttcagacaac agataacaaa caaaatcagc gacttctgct aatcttccgg
    12061 cgcgcccaat aggaatggcg tttttggtat agccttcacg cagctgctcg acggtgatat
    12121 tgcgcgtcca cgccagcgcc tcttcatatt ccggcgtgcg tagcccggtt ttttccagaa
    12181 tcccgggcgc gataccgacc acacggatac cgtgtttacc cagctctttc gaccaggagc
    12241 gcgtgaagct attaagcgcg gctttggtag cggcgtaaca gctttggcct tctgagcctt
    12301 ccagtccact ttctgaggaa acattcacaa tcacgccatc atgttgtttg accatctgtc
    12361 gcgccaccgc ctgcgacatc agaaaaacgc ctttctggtt gatattgacc attttttcga
    12421 atgcagcttc gttgagttca tactgcccgg caggcgcttt ctcatcgacc agcagacgcg
    12481 ggaaattgac cccggcgtta ttgaccagac cgtcgatgcg accaaaacgc tggataattt
    12541 ccgctaccgt atgatttacc tctttggcgc tggaaatatc ggtcggccaa aactgatagc
    12601 ctttatgacc ttcatattga ccatcgccac cgtgaatatc taccatctgt acgtttgcgc
    12661 cttgtgctaa taattcctct acaatcgcca gaccaatacc ggatgcgccg ccggtgacga
    12721 taatgatttt atcctgcaaa tttaaccacg tttgcataac agactcctga ttttagataa
    12781 tagggacccg ccgtaatttt tattacggta aacatcggga agatttttat ttcagtaata
    12841 gttcagctgt gctgctattc gttaccagac aattaatata ttttccacgc agtgcaccaa
    12901 taattccgct gtatttttct tcactcatgg caatgccaat ggaatatcgc gcctgcttta
    12961 atttattcat ttcgatagag agcgtttttt cgctcatatt cgtttcaacc attgcgccgt
    13021 gaatatcaaa aaagcgcgag caaatatcgc cagcaacctg gcgggcattc aggtcgtcac
    13081 tctcttcacc accataaaac gcatgccagt tagcgccgtc gcgaatggcc ggtgagccaa
    13141 ttcccaccag ggcgacatcc agattatccc agtaggcaga gatggtttta aagtgctgag
    13201 actgcatgat cccattacga attaatgggt tatccagcag agccggaaaa tccgcgagat
    13261 gcgattcccc tttcagcttc gctgccgcgc tgtaggttaa tgtgtttaca tgatagcggc
    13321 tttcgagttt accggacggt ccgccaataa ttggtacgca gattaactgc cgcgattgcc
    13381 ccgcctgcgg caagttttca accagtgcgc tcaccgcgcg accccaggaa aaaccgacaa
    13441 tatcgccagg ctccagcaag cgatccagca gttgcgcgcc gtgtaacccc atcatcgcca
    13501 gttgagtctc ttcatcctca tcatttcccg acaccaccac aacgtctttc aggccaaact
    13561 tttgcttcag ttgctgctcc agccagagat tttcgttgta gtcatagttg atggcgatgg
    13621 tgacaattcc ctgatcgcgg cctcgtttaa gcaagcggct gatggtggtg cggtaaatac
    13681 gcagttcgcg cgcgatttgc gcctgcgtca tatcctgttc gtaatagagt tgggcaatct
    13741 tcacaatcaa acggatatcg tcactgtttt ccattgccgc ctcctggttc tgcacatttg
    13801 tgcaaatacg ctttctcacc gcttgatctg atataacccc atcatgaggg cctttcaaag
    13861 cgatttttca ctctttgcat ggggagaaat agcgctttct cttgcacatt tgtttctctg
    13921 cgtagtagtc atcagcacag gtgtgcagcg attcgtaata tgttgattat ccttattttg
    13981 ccaggcaaat ttgcatcgta cttccggaga cacttttgtg ataaaaggcg tggcagatca
    14041 catttaattt cgacgttgcc gcataaccct gtttttcccc gctaagaggt acggtatatt
    14101 ccgcgtttta caggagaatt tatgctgccc gactcatcag tccgtttaaa taaatacatc
    14161 agcgaaagcg gaatttgctc acgccgcgaa gcggatcgct atatcgagca aggcaatgtg
    14221 ttccttaatg gcaagcgagc caccattggc gatcaggtga aacccggcga cattgtgaaa
    14281 gtaaacggtc agttgattga gcctcgggaa gccgaagatt tggtacttat cgccctgaac
    14341 aagcccgtcg gtattgtcag caccaccgaa gatggcgagc gcgataacat tgtcgatttc
    14401 gttaaccaca gcaaacgcgt gttcccgatt ggccgcctgg ataaagactc ccaggggctg
    14461 attttcctca ccaatcacgg cgatctggtg aataagatcc tccgtgcggg taacgatcat
    14521 gagaaagagt atctggtgac ggtcgataaa ccgattaccg atgaatttat tcgtggtatg
    14581 ggggcggggg tgccgatcct cgggacggtg acgaaaaagt gcaaagtcaa aaaagaagcg
    14641 ccgtttgtct tccgcattac cctggtgcag gggctgaacc gtcagatccg gcgcatgtgc
    14701 gagcatttcg gctatgaagt gaaaaagctg gaacgtacgc gcattatgaa cgtcagttta
    14761 agcggcattc cgctgggtga gtggcgcgat ttaactgacg atgagttaat cgacctgttt
    14821 aaactcattg aaaattcctc ttccgaggta aaacctaaag cgaaggctaa accgaaaaca
    14881 gcgggcatca aacgtccagt cgttaagatg gaaaaaacgg cggaaaaagg cggtcgcccg
    14941 gcgtccaacg gtaagcgttt tacctcgccg gggcgtaaaa agaagggacg ctaatcagcg
    15001 cctgccgcgc gtcgacgtat ttgctgacca gctaaatgac gcttcaggat ccggtttata
    15061 agcctgcttt ttcttcaact ggcgggcttt tttcgcttca gcctcacgct cgaccattaa
    15121 tttatcgatg tactctttct taatgctgtt ggtttcggag ttggtcagta cgcgaccatg
    15181 cgcaatccgc gcccgatcca gcagtgtttt aagttcacgc tgctcgcgtt cagtcatctc
    15241 tttttgggta atacggggga gtgccatgat ggtgccctca gtgagccaga gagttcagtg
    15301 tacgggaatt cgtttg
//
LOCUS       DAZGJW010000113        15291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZGJW010000113 DAZGJW010000000
VERSION     DAZGJW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.113"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            78..1367
                     /gene="hpaG"
                     /locus_tag="ACHKIK_003562"
     CDS             78..1367
                     /gene="hpaG"
                     /locus_tag="ACHKIK_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFO9084662.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGCLVNRIVSEETAK"
     gene            1364..2830
                     /gene="hpaE"
                     /locus_tag="ACHKIK_003563"
     CDS             1364..2830
                     /gene="hpaE"
                     /locus_tag="ACHKIK_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFO9084663.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2832..3683
                     /gene="hpaD"
                     /locus_tag="ACHKIK_003564"
     CDS             2832..3683
                     /gene="hpaD"
                     /locus_tag="ACHKIK_003564"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFO9084664.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3693..4073
                     /locus_tag="ACHKIK_003565"
     CDS             3693..4073
                     /locus_tag="ACHKIK_003565"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFO9084665.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQHDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4184..4987
                     /gene="hpaH"
                     /locus_tag="ACHKIK_003566"
     CDS             4184..4987
                     /gene="hpaH"
                     /locus_tag="ACHKIK_003566"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFO9084666.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4998..5786
                     /gene="hpaI"
                     /locus_tag="ACHKIK_003567"
     CDS             4998..5786
                     /gene="hpaI"
                     /locus_tag="ACHKIK_003567"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFO9084667.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAIKPDVY"
     gene            5961..7337
                     /gene="hpaX"
                     /locus_tag="ACHKIK_003568"
     CDS             5961..7337
                     /gene="hpaX"
                     /locus_tag="ACHKIK_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFO9084668.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVATLLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7347..8237
                     /gene="hpaA"
                     /locus_tag="ACHKIK_003569"
     CDS             7347..8237
                     /gene="hpaA"
                     /locus_tag="ACHKIK_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFO9084669.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFHMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8488..10050
                     /gene="hpaB"
                     /locus_tag="ACHKIK_003570"
     CDS             8488..10050
                     /gene="hpaB"
                     /locus_tag="ACHKIK_003570"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFO9084670.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10068..10580
                     /locus_tag="ACHKIK_003571"
     CDS             10068..10580
                     /locus_tag="ACHKIK_003571"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFO9084671.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSIT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10973..13123
                     /gene="btsT"
                     /locus_tag="ACHKIK_003572"
     CDS             10973..13123
                     /gene="btsT"
                     /locus_tag="ACHKIK_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO9084672.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13254..13457
                     /locus_tag="ACHKIK_003573"
     CDS             13254..13457
                     /locus_tag="ACHKIK_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO9084673.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13468..14424
                     /gene="yjiA"
                     /locus_tag="ACHKIK_003574"
     CDS             13468..14424
                     /gene="yjiA"
                     /locus_tag="ACHKIK_003574"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO9084674.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(14511..14702)
                     /locus_tag="ACHKIK_003575"
     CDS             complement(14511..14702)
                     /locus_tag="ACHKIK_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO9084675.1"
                     /translation="MSMVKHKRGTLYTLDVQQEAELMALVNKSDEDIDYSDIPASGSE
                     AWSDAKRGNFLPDPGIHAQ"
     gene            complement(14742..>15291)
                     /locus_tag="ACHKIK_003576"
     CDS             complement(14742..>15291)
                     /locus_tag="ACHKIK_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084676.1"
                     /translation="LIRSMIIACIPIIYLFASSCASSLFLSLSNLNITLTPWVEYFWK
                     GMAFMLLFFMLMQLVIYFAFLTLWAKLSVYRLFILAGAFIVSTILVAFASKNVEGISY
                     YVLKSTIDFEWRNQVKCGELNISRPDERYFGFNTDKYTVFYSNREGKWGVNELKCKKG
                     SDRRDAYSIENVSEYNVPGWLK"
BASE COUNT         3611 a         4167 c         4131 g         3382 t
ORIGIN      
        1 tgccaaacta cgcaatcggg aataaaagca attattaaca tattaatgat taagatgatc
       61 gaatccgagg agtggtaatg aaaggcacta tcttcgccgt agcgttgaac catcgcagcc
      121 aacttgatgc atggcaggaa gcgttccagc aatcccccta caaagccccg cctaaaactg
      181 cggtctggtt tattaaaccg cgcaatacgg tgattggttg cggtgaaccg attccctttc
      241 cacagggtga aaaggtactg agcggtgcga ctgttgcgct gattgtggga aaaacggcga
      301 cgaaagtacg tgaagaagat gcggcagagt acatcgccgg atatgcgctg gctaacgacg
      361 tcagcctgcc ggaagagagc ttttaccgcc cggcaatcaa agcaaaatgt cgtgatggat
      421 tctgccccat tggcgaaacc gtggctctca gcaatgtcga taatctgacc atctataccg
      481 agatcaacgg gcgtcctgcc gatcactgga ataccgccga tttacaacgt aacgccgcgc
      541 agttgctgag cgccctgagc gaatttgcca cactgaatcc aggcgatgcc attctgctcg
      601 gcacgccaca ggcgcgcgtg gaaatacagc caggcgatcg cgttcgtgtt ctcgcagaag
      661 gtttcccgcc gctggaaaat ccggtagtgg acgaacgtga agtgaccacg cgcaagagct
      721 tcccaacgca gccacacccg cacggcacgc tgtttgccct cggcctgaac tacgccgacc
      781 acgccagcga actggaattt aagccaccgg aagaaccgct ggtgttcctg aaagcgccaa
      841 ataccctcac tggcgataac cagacctccg tgcgtccaaa caatattgaa tacatgcact
      901 atgaagcgga gctggtggta gttattggca aacaggcgcg taacgtcagc gaagccgatg
      961 ccatggatta tgtcgcgggc tacaccgtgt gtaacgacta cgccattcgc gactatctgg
     1021 aaaactacta ccgccctaac ctgcgggtaa aaagccgcga cggactgacg ccgatgcttt
     1081 caaccatcgt gccgaaagag gcgatcccgg acccgcataa tctgaccctt cgcaccttcg
     1141 tcaacggcga gttacgccag caaggcacca ccgccgatct gatcttcagc gtgcccttcc
     1201 tgatcgccta cttaagcgaa tttatgaccc tgaatccggg cgacatgatc gccaccggca
     1261 caccaaaagg cttatctgac gtggtgcctg gcgatgaagt agtggtggaa gtagaaggcg
     1321 tgggctgcct ggtgaaccga attgtgagtg aggaaacagc gaaatgaaaa aagtaaatca
     1381 ttggatcaac ggcaaaaatg ttgcaggtaa cgactacttc cagaccacca atccggcaac
     1441 gggtgaagtg ctggcggatg tggcctctgg cggtgaagcg gagatcaatc aggcggtagc
     1501 ggcagcgaaa gaggcgttcc cgaaatgggc caatctgccg atgaaagagc gtgcgcgcct
     1561 gatgcgccgt ctgggcgatc tgatcgacca gaacgtgcca gagatcgccg cgatggaaac
     1621 cgcggacacc ggcctgccga tccatcagac caaaaatgtg ttgatcccac gcgcttccca
     1681 caactttgaa tttttcgcgg aagtctgcca gcagatgaac ggcaagacct atccggttga
     1741 cgacaagatg ctcaactaca cgctggtgca gccggtgggc gtttgtgcgc tggtatcgcc
     1801 gtggaacgta ccgtttatga ccgccacatg gaaggtcgcg ccgtgtctgg cgctgggcaa
     1861 tactgcggtg ctgaagatgt cagaactctc cccgctgacc gctgaccgcc tgggtgagct
     1921 ggcgctggaa gccggtattc cggcgggcgt gctgaacgtg gtacagggct acggcgcaac
     1981 cgcaggggat gcgctggtcc gtcatcatga cgtgcgtgcc gtgtcgttca ccggcggtac
     2041 cgccaccggg cgcaacatca tgaaaaacgc cgggctgaaa aaatactcca tggaactggg
     2101 cggaaaatcg ccagtgctga tttttgaaga tgccgatatt gaacgcgcgc tggacgccgc
     2161 cctgttcacc atcttctcga tcaacggcga gcgctgcacc gccggttcgc gcatctttat
     2221 tcagcaaagc atctacccgg aattcgttaa acgctttgcc gaacgcgcca accgtctgcg
     2281 cgtgggcgat ccgaacgatc cgaataccca ggttggggca cttatcagcc agcaacactg
     2341 ggaaaaagtc tccggctata tccgtctcgg cattgaagaa ggcgcaaccc tgctggcggg
     2401 cggcccggat aaaccgtctg acctgcctgc gcacctgaaa ggcggcaact tcctgcgccc
     2461 aacggtgctg gcagacgtag ataaccgtat gcgcgtcgcc caggaagaga ttttcgggcc
     2521 ggtcgcctgc ctgctgccgt ttaaagacga agccgaaggc ttacgcctgg caaacgacgt
     2581 ggagtacggc ctcgcgtcgt acatctggac acaggatgtc agcaaagtgt tacgcctggc
     2641 gcgtggcatt gaagcaggca tggtgttcgt caatacccag aacgtccgcg acctgcgcca
     2701 gccgtttggc ggcgtaaaag cctccggcac cgggcgtgaa ggcggtgagt acagcttcga
     2761 agtgttcgcg gaaatgaaga acgtctgcat ttccatgggc gaccatccaa ttccgaaatg
     2821 gggagtctga tatgggtaag ttagcgttag ccgccaagat cacgcacgta ccgtcgatgt
     2881 atctctctga actaccagga aaaaaccacg gttgtcgcca gggtgcgatc gacgggcata
     2941 aagagatcag caagcgttgc cgggaaatgg gcgtcgatac cattatcgtt ttcgacaccc
     3001 actggctggt caacagtgct tatcacatca actgtgcaga ccattttgaa ggcgtctaca
     3061 ccagtaacga actgccgcat tttattcgtg acatgaccta caactacgag ggcaacccgg
     3121 agttggggca gctaattgcc gatgaagcct taaagctcgg cgtgcgggca aaagcgcaca
     3181 atattcccag cctgaaactg gaatacggca cgctggtgcc gatgcgctac atgaatgaag
     3241 acaagcactt caaagtggtc tccatttcgg ctttctgcac ggttcacgat tttgccgaca
     3301 gccgcaagct gggcgaagcg attctgaaag cgatcgaaca gtacgacggc accgtggcgg
     3361 tccttgccag cggttcgtta tcgcaccgct ttattgacga tcagcgtgcg gaagaaggga
     3421 tgaacagcta cacccgcgag ttcgaccgcc agatggacga gcgcgtggtg aagctgtggc
     3481 gcgaaggcca gttcaaagag ttttgcaata tgctgccgga gtacgccgac tactgctacg
     3541 gcgaaggcaa tatgcacgac acggtgatgc tgctggggat gctcggctgg gataaatacg
     3601 acggcaaggt ggagtttatc accgagctat tcccaagctc tggcaccggt caggttaacg
     3661 ctgttttccc gcttcccgcg taaggacgtt ttatgccgca ctttatcgtt gaatgcagtg
     3721 ataacatccg cgaagaagcc gacctgccgg ggttgttcgc caaagtgaat ccgacgctgg
     3781 cagccacggg tatttttccg ctggcgggta ttcgcagccg cgtgcattgg gtcgatacct
     3841 ggcagatggc cgacgggcag catgattatg ccttcgtgca tatgacgttg aaaatcggcg
     3901 caggtcgcag cctggaaagc cgccagcagg cgggtgaaat gctgtttgaa ctgattaaaa
     3961 cgcacttcgc cgccctgatg gagagccgcc tgctggcgtt gtcgtttgag attgaagagc
     4021 tgcatccgac gctgaatttt aaacaaaaca acgtgcacgc attgtttaag tagcgcgcag
     4081 attgcccggt ggcgctgcgc ttaccgggcc tacaaaaccc caaaccgtac accgtaggcc
     4141 ggataaggcg cagccgcatc cggcaatgcc acaggatatc gctatgttcg ataaacacac
     4201 ccacaccctg atcgcccagc gtctggatca ggcagaaaaa cagcgcgaac agatccgcgc
     4261 gatctcgctg gattacccgg agatcaccat cgaagacgct tacgcggtgc agcgtgaatg
     4321 ggttcgcctg aaaatcgccg aaggtcgcac gctgaaaggc cacaaaatcg gcctgacctc
     4381 gaaagcgatg caggcaagct cgcagatcag cgaaccggat tacggcgcgc tgctggatga
     4441 catgttcttc cacgatggca gcgatatccc gaccgatcgc tttatcgtgc cgcgcattga
     4501 agtggagctg gctttcgtgc tggcaaaacc gctgcgtggg ccaaactgca cgctgttcga
     4561 cgtctacaac gccacggact acgtgatccc ggcgctggag ctgatcgacg ctcgctgcca
     4621 caacatcgat ccggaaaccc agcgcccgcg caaagttttc gacaccattt ctgataacgc
     4681 cgccaacgcc ggggtgatcc tcggaggtcg tccgatcaaa ccagacgaac tggatctgcg
     4741 ttggatctcc gccctgatgt atcgcaatgg cgtgattgaa gaaaccggcg tcgccgctgg
     4801 cgtgctgaat catccggcaa acggcgtggc ctggctggcg aacaaactcg caccgtatga
     4861 cgtacaactg gaagccgggc aaatcattct cggtggttcg ttcacccgcc cggttccggc
     4921 gcgtaagggc gacaccttcc acgtcgatta cggcaacatg ggttccatta gctgccgctt
     4981 tgtttaagga gagaacgatg gaaaacagct ttaaagcggc gctgaaagca ggccgtccgc
     5041 agatcggatt atggctgggg ctgagtagca gctacagcgc agagttactg gccggagcag
     5101 gattcgactg gttgttgatc gacggtgagc acgcgccgaa taacgtgcaa accgtgctca
     5161 cccagctaca ggcgattgcg ccctacccca gccagccggt agtacgtccg tcgtggaacg
     5221 atccggtgca aatcaaacaa ctgctggacg tcggcacaca aaccttgctg gtgccgatgg
     5281 tacaaaacgc tgacgaagcc cgtgaagcgg tacgcgccac ccgttatccc cccgccggta
     5341 ttcgcggtgt gggcagtgcg ctggcccgag cctcgcgctg gaatcgcatt cctgattacc
     5401 tgcaaaaagc caacgatcaa atgtgcgtgc tggtgcagat cgaaacgcgt gaggcaatga
     5461 agaacttacc gcagattctg gacgtggaag gggtcgacgg cgtgtttatc ggcccggcgg
     5521 atctgagcgc cgatatgggt tatgccggta atcctcagca cccggaagta caggccgcga
     5581 ttgagcaggc gatcgtgcag atccgcgaag cgggcaaagc gccggggatc ctgatcgcca
     5641 atgagcaact ggcaaaacgc tatctggaac tgggcgcgct gtttgtcgcc gtcggcgttg
     5701 acaccaccct gctcgcccgc gccgccgaag cgctggcagc acggtttggt gcgcaggcta
     5761 cagcgattaa gcccgacgtg tattaatgcc ggatgcgctg cgcttatccg gcctacactc
     5821 gcaccaaacc gtaggccgga taaggcgttt acgccgcatc cggcaaaaag ccgtaccaaa
     5881 tcgcggaccg gataaggcgt ttgtgcgcat ccgccataaa ccttgtcgta ccctacaaaa
     5941 atcccattag aggaagtaaa atgagcgaca cctcacctgc cataccggag agtatcgatc
     6001 cggcgaatca gcataaagcg ctgactgccg gacaacaggc ggttattaag aagctatttc
     6061 gccgcctgat cgtctttctg ttcgtgctgt ttatcttctc gttccttgat cgcatcaaca
     6121 tcggctttgc cggactcacg atgggacgcg acctcggtct gagcgccacc atgtttggcc
     6181 tcgctaccac cttgttctac gccgcttacg tcatcttcgg cattcccagc aacattatgc
     6241 tgagtattgt cggtgcacgg cgctggatcg ccaccatcat ggtgctttgg ggcatcgcct
     6301 ctactgccac catgtttgcc actggcccca ccagcttata cgtactgcgt atactggttg
     6361 gcattaccga agccggcttt ctgcctggca ttctgctgta tttaaccttc tggtttccgg
     6421 cctatttccg cgcccgtgcc aacgccttgt ttatggtagc aatgccggta acgacagcgt
     6481 tgggatcgat cgtttccggc tacattttgt cgctggatgg cgtaatggca ttaaaaggct
     6541 ggcagtggct gtttttgctg gaaggcttcc cgtcggtatt actcggcgtc atggtgtggt
     6601 tctggcttga tgactcaccg gacaaagcta agtggctgac gaaagaagac aaaaaatgcc
     6661 tgcaagagat gatggataac gatcgtctga cgctggttca gccagaggga gccatcagcc
     6721 accacgccat gcaacaacgc agtatgtggc gggagatctt cactccggtg gtgatgatgt
     6781 ataccctggc gtatttctgc ctgaccaaca cacttagtgc gatcagcatc tggacaccgc
     6841 agatcctgca aagctttaat cagggcagca gtaatatcac catcggcctg ctggccgccg
     6901 taccgcagat ttgtaccatt ctcgggatga tctactggag ccgtcactca gatcgccgcc
     6961 aggaacgaag gcatcacacc gcccttcctt atttgttcgc tgccgctggt tggttactgg
     7021 cttcggcaac tgatcacaac atgatccaga tgctggggat cattatggct tcgaccggat
     7081 cattcagcgc aatggcgatt ttctggacaa caccggatca gtccatcagc ctgcgggcac
     7141 gagcgatcgg tattgcggtg atcaacgcca ctggcaacat tggttcagcg ttaagtccgt
     7201 ttatgatcgg ctggttgaaa gatctgaccg gcagctttaa cagtggattg tggtttgttg
     7261 ccacgctgct ggtgattggt gcggggatta tctgggcgat tccaatgcag tcctcccgtc
     7321 cgcgagcgac cccgtaagga acgacgatgt gtgaccgtca gattgccaat attgatatca
     7381 gcaaagagta cgatgaaagc ctgggcacgg acgatgtgca ttatcagtcg ttcgcccgca
     7441 tggcggcctt ttttggccgc catatgctgc cacatcgcca cgaacagtac tttcagatgc
     7501 atttcctcaa tagcggacag attgagctac agcttgacga tcatcgctac tcggtggaag
     7561 cgcccctgtt tgtcctgacg ccgccgtcag tacctcatgc gtttattacg gagtctgatg
     7621 ctgacggtca tgtattgacg gtacgggaag atctgatctg gcccctgctg gaagttcttt
     7681 atccgggcac tcgggaaacc ttcggcctgc cggggatttg cctgtcactg gcagataaac
     7741 ccgacgaact ggcggcgctg gaacactatt ggcaactgat agagcgggaa tcggtagaac
     7801 aactgcctgg tcgggaacac accctgacgt tactggcaca ggcagtgttc accctactgc
     7861 tgcgtaacgc aaaactcgac gaccatgccg ccagcggaat gcgcggagaa ttaaaactgt
     7921 tccagcgttt tcatatgctg attgaaagcc attttcatca gcactggaca gtaccggatt
     7981 acgctaacga attgcatatc accgaatcac gcctcactga catctgccgc cgctttgcca
     8041 accgtccgcc aaaacggttg attttcgaca ggcagctgcg agaagccaag cggctgctgc
     8101 tgttttctga taacgccgtg aacaatattg cctggcaact cggttttaag gatccggctt
     8161 attttgcgcg cttttttaat cgcttagtcg gttgctcgcc cagtgcttat cgcgccaaaa
     8221 aagtacctgt gacgtgaaaa tcccttagcc ttaacgggaa accaggcacc acctgctatt
     8281 cccctttctc cgcctggagc aaggggattt agtaacgtta aaaatcgcta aaagcgacct
     8341 cgatcacaaa accgtacccg atctgaaaag taccagccat tgtcccaaaa gtgtcttccc
     8401 gtaatcgccc ctgcaagctt caattgaata acaaaaacaa cacacaaaat taacaatcgt
     8461 attccgatta atactgtaga ggtcgacatg aaaccagaag atttccgcgc cagtacccaa
     8521 cgtccgttca ccggggaaga gtatctgaaa agcctgcagg atggtcgcga gatctatatc
     8581 tatggcgagc gagtgaaaga cgtcaccact catccggcat ttcgtaatgc ggcagcgtct
     8641 gttgcccaac tgtacgacgc gctacacaaa ccggagatgc aggactctct gtgctggaac
     8701 accgacaccg gtagcggcgg ctatacccat aaattcttcc gcgtggcgaa aagtgccgac
     8761 gacctgcgcc agcaacgcga cgccatcgct gagtggtcac gcctgagcta tggctggatg
     8821 ggccgtaccc cagactacaa agccgctttc ggttgcgcac tgggcgcgaa tccgggcttt
     8881 tacggtcagt tcgagcagaa cgcccgtaac tggtataccc gtattcagga aactggcctc
     8941 tactttaacc acgcgattgt taacccaccg atcgatcgtc atttgccgac cgataaagtg
     9001 aaagacgttt acatcaagct ggaaaaagag actgacgccg ggattatcgt cagcggtgcg
     9061 aaagtggttg ccaccaactc ggcgctgact cactacaaca tgattggctt cggctcggca
     9121 caagtgatgg gcgaaaaccc ggacttcgca ctgatgttcg ttgcgccaat ggatgccgat
     9181 ggcgtgaaat taatctcccg cgcctcttat gagatggtcg cgggtgctac cggctcgccg
     9241 tacgactacc cgctctccag ccgctttgat gagaatgatg cgattctggt gatggataac
     9301 gtgctgatcc catgggaaaa cgtactgatc taccgcgatt tcgatcgctg ccgtcgctgg
     9361 acgatggaag gcggttttgc ccgtatgtat ccgctgcaag cctgtgtgcg cctggcagtg
     9421 aaactagact tcattacggc actgctgaaa aaatcactcg aatgtaccgg caccctggag
     9481 ttccgtggtg tgcaggccga tctcggtgaa gtggtggcgt ggcgcaacac cttctgggca
     9541 ttgagtgact cgatgtgttc agaagcgaca ccatgggtca acggtgctta tttaccggat
     9601 catgccgcac tgcaaaccta tcgcgtactg gcaccaatgg cctacgcgaa gatcaaaaac
     9661 attatcgaac gcaacgttac cagtggcctg atctatctcc cttccagtgc ccgtgacctg
     9721 aacaatccgc agatcgacca gtatctggcg aagtatgtgc gcggttcgaa cggtatggat
     9781 cacgtccagc gcatcaagat cctcaaactg atgtgggacg ccattggcag cgagtttggt
     9841 ggtcgtcacg aactgtatga aatcaactac tccggtagcc aggatgagat tcgcctgcag
     9901 tgtctgcgcc aggcacaaag ctccggcaat atggacaaga tgatggcgat ggttgatcgc
     9961 tgcctgtcgg aatacgacca gaacggctgg actgtgccgc acctgcacaa caacgacgat
    10021 atcaacatgc tggataagct gctgaaataa cgcagcagga ggttaagatg caattagatg
    10081 aacaacgcct gcgctttcgt gacgcaatgg ccagcctgtc ggcagcggta aatattatca
    10141 ccaccgaggg cgacgccgga caatgcggaa ttacggcaac ggccgtctgc tcgatcacgg
    10201 atacaccacc gtcgctgatg gtgtgcatta acgccaacag tgcgatgaac ccggtttttc
    10261 agggcaacgg taagttgtgc gtcaacgtcc tcaatcatga gcaggagctg atggcccgcc
    10321 acttcgcggg catgacaggc atggcgatgg aagagcgttt tagcctctca tgctggcaaa
    10381 aaggtccgct ggcacagccg gtgctgaaag gttcgctggc cagtcttgaa ggtgagatcc
    10441 gcgatgtgca ggcaattggc acacatctgg tgtatctggt ggagattaaa aacatcatcc
    10501 tcagtgcaga aggtcacgga cttatctact ttaaacgccg tttccatccg gtgatgctgg
    10561 aaatggaagc tgcgatttaa gctccagccc cttcacgcat tcgtcgaagg ggcattttta
    10621 ttgtgatctc aatcacatat ccacccgatg aataattctt acccaaccgc tcaccgccat
    10681 tcctgaccgc ttacatccct aaaataacca ctcagttatt taccttactt tacgcgtgcg
    10741 taactctggc aacatcactg caggatagcg gtcaatttac ctcctcaaac gcaacgcaaa
    10801 cctagaacgg cttcggccta tgattaacaa attactctat ggattttggt ttgcaggaag
    10861 gcagcaagtg agtgaatccc cgggagctta taacagtaag tgacaggggt gaacgaacgc
    10921 agctgccgca cctgtaagcc aaaagacgac gagtaaaatg ccaggtttta ctatggatac
    10981 gaaaaaacta ttcaagcaca taccctgggt gattctcgga atcatcggtg cattctgcct
    11041 cgcggtagtt gcattacgtc ggggggagca cgtcagcgcc ctgtggatcg tggtcgcctc
    11101 tgtgtcggtg tatctggtgg cgtatcgcta ctacagtctg tatatcgccc agaaggtgat
    11161 gaaactcgac cccacgcgcg cgacgcctgc ggttattaac aacgacggtc tgaactacgt
    11221 tccgaccaac cgttacgtgt tgtttggtca ccacttcgcc gctatcgccg gtgctggtcc
    11281 gctggttggt ccggttctcg ccgcgcagat gggctacctg cccggcacgc tgtggctgct
    11341 ggcgggggta gtgctggccg gtgcggtaca ggactttatg gtgctgttta tctcctctcg
    11401 ccgtaacggc gcatctctcg gtgagatgat caaagaagag atgggaccag taccggggac
    11461 tatcgcgctg tttggctgtt tcttaatcat gatcatcatc cttgccgtcc tggcgctgat
    11521 tgtggttaaa gccctggcag aaagtccgtg gggtgtcttc accgtttgct caaccgtacc
    11581 gattgcgctg tttatgggta tctacatgcg ctttatccgt ccggggcgtg tgggtgaagt
    11641 ctctgtaatc ggtatcgtgc tgctggttgc ctctatctac ttcggtggcg tgattgctca
    11701 cgatccgtac tggggtccgg cactgacctt taaagacacc accattacct tcgcgctgat
    11761 tggctatgcg tttgtttccg cactgctgcc ggtgtggctg atcctcgcac cgcgcgacta
    11821 tctggcaacc ttcctgaaaa tcggcgttat cgtcggcctg gcgttgggta tcgtggtgct
    11881 gaacccggaa ctgaaaatgc ctgcgatgac ccagtacatt gacggtactg gcccgctgtg
    11941 gaaaggcgct ctgttcccgt tcctgttcat caccatcgcc tgtggtgcgg tatctggctt
    12001 ccacgcgctg atctcttccg gtacgacgcc gaaactgctg gctaacgaaa ccgacgcgcg
    12061 tttcattggc tacggcgcaa tgctgatgga gtccttcgtg gcgattatgg cgctggttgc
    12121 agcgtccatc atcgaaccgg gtctttactt cgcgatgaac accccgcctg cgggccttgg
    12181 catcaccatg cctaacctgc atgaaatggg tggcgagaac gcgccgatca tcatggcgca
    12241 gctgaaagac gttaccgcac acgcggcggc gaccgtcagc tcctggggct tcgtgatttc
    12301 gccagagcag atcctgcaaa ccgcgaaaga cattggtgag ccttctgtcc tgaaccgtgc
    12361 aggtggtgcg ccgacactgg cggtaggtat cgcgcacgtg ttccacaaag tgctgccgat
    12421 ggctgacatg ggcttctggt atcacttcgg tattctgttc gaagccctgt tcatcctgac
    12481 cgcgctggat gcgggtaccc gttctggccg ctttatgctg caggacctgc tgggtaactt
    12541 catcccgttc ctgaaaaaaa ccgactctct ggttgctggt atcatcggta ctgcgggctg
    12601 tgtgggtctg tggggctacc tgctgtatca gggcgtggtc gatccgctgg gcggcgttaa
    12661 gagcctgtgg ccgctgttcg gtatctccaa ccagatgctg gcagccgtag cgctggtact
    12721 gggcaccgtt gtgctgatta agatgaagcg cacccaatac atctgggtaa ctgttgttcc
    12781 ggctgtatgg ctgcttatct gcaccacctg ggcgctgggc ctgaaactgt tcagcaccaa
    12841 cccgcagatg gaaggcttct tctacatggc aagccagtac aaagagaaga ttgctaacgg
    12901 tactgacctg acggcacagc agattgccaa catgaaccac atcgttgtga acaactacac
    12961 caacgcgggc ctgagtattc tgttcctgat tgtggtgtac agcatcatct tctacggttt
    13021 caaaacctgg cttgcggtgc gtaacagcga caaacgtact gacaaagaaa caccgtacgt
    13081 tccaatcccg gaaggcggcg tgaagatctc ttcgcaccac taaccggttg ccggatgcgt
    13141 tgcgcgtatc cggcctacaa agtgtctatc gtaggccgga taaagcgttt acgccgcatc
    13201 cggcaaatcc ctagcccgga ccttccgggc tttacgcata tctggaaggc aaaatgtttg
    13261 gtaacttagg acaggcaaaa aaatatctcg gccaggcggc gaagatgttg attggcattc
    13321 cagactacga caactacgtc gagcatatga agaccaacca tcccgacaag ccgtacatga
    13381 gctatgaaga attcttccgc gaacgccaga acgcgcgcta cggcggcgat ggtaaaggcg
    13441 gtatgcgctg ttgttaatgg agagagcatg aacccgattg cagttaccct actcaccggt
    13501 tttttaggcg cgggcaaaac caccctgctg cgccatattc ttaacgaaca acacggctac
    13561 aagattgccg tgattgaaaa cgaattcggc gaagtctccg ttgatgatca actgattggc
    13621 gaccgcgcca cgcagatcaa aacgctgacc aacggctgca tctgctgttc gcgctccaac
    13681 gagctggaag acgcactact cgacctgctg gataatctcg acaagggcaa tattcagttc
    13741 gaccgtctgg tcattgaatg caccggcatg gccgatcccg gtccgattat tcagaccttt
    13801 ttctcccatg agattttatg ccagcgttac ctgctggacg gcgtgattgc gctggtggat
    13861 gcggtacatg ccgatgagca gatgaaccag ttcaccatcg cccagtccca ggttggctac
    13921 gccgaccgta ttctgctgac caaaaccgac gtcgcaggcg aagcagaaaa actgcgtgaa
    13981 cgcctggcgc gcatcaacgc ccgtgcaccg gtctacaccg tcacccacgg cgacatcgac
    14041 ctgggcctgc tattcaacac caacggtttt atgctggaag agaacgtcgt cagcaccaaa
    14101 ccgcgcttcc actttatcgc ggataaacaa aacgatattt cgtcgattgt ggtggaactg
    14161 gattacccgg tagatatcag cgaagtttcc cgggtgatgg aaaacctgct gctggaatcg
    14221 gcagataaac tgctgcgtta caaagggatg ctgtggattg acggcgaacc taaccgcctg
    14281 ctattccagg gcgtccagcg cctctacagc gccgactggg accgcccgtg gggcgatgaa
    14341 aaaccgcata gcacgatggt gtttatcggg attcagctgc cggaggaaga gattcgggcg
    14401 gcgtttgcgg ggttgaggaa gtaatcgtga aaggttgagt gtaaagcagc tcaatgcccc
    14461 atttagcagt gaggggggaa acagaacgac gtgctcccct ccgctaagtt ttattgcgcg
    14521 tgaatacctg gatctggcaa aaaattgccg cgtttggcgt cagaccatgc ttcgctacca
    14581 gaagcaggaa tgtcgctgta atcaatgtct tcgtcggatt tattaaccaa agccatgagt
    14641 tcagcttctt gctgcacatc aagtgtgtag agtgtgcccc gtttatgttt aaccatgctc
    14701 atttatgacc agaaccttta cgatttaatt ataaagatga cttatttcag ccatccaggc
    14761 acgttatatt ctgagacatt ctcaatggaa tatgcatctc tcctgtcaga acctttctta
    14821 cacttcaact cattgacccc ccatttaccc tctctattgg aataaaatac agtatattta
    14881 tcggtattaa acccgaaata tctttcatcc ggacgtgaaa tattcaactc tccacattta
    14941 acctgattac gccattcaaa atctatggtg ctttttaaaa cgtagtacga aataccttct
    15001 acattttttg aagcaaaagc cactaaaata gtagatacga taaatgctcc ggcaagtata
    15061 aacaacctat atacacttaa tttagcccac agagttaaaa aagcaaaata gataacaagt
    15121 tgcattaaca taaagaaaag caacataaaa gccatccctt tccagaaata ctcaacccat
    15181 ggcgttagtg tgatattgag attactcaaa cttaaaaaca aactggaagc acaactactt
    15241 gcaaacaaat atataattgg tatgcaagct ataatcatgg acctaataag t
//
LOCUS       DAZGJW010000114        15107 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZGJW010000114 DAZGJW010000000
VERSION     DAZGJW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.114"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            116..358
                     /gene="yjbE"
                     /locus_tag="ACHKIK_003577"
     CDS             116..358
                     /gene="yjbE"
                     /locus_tag="ACHKIK_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO9084677.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            472..1110
                     /gene="yjbF"
                     /locus_tag="ACHKIK_003578"
     CDS             472..1110
                     /gene="yjbF"
                     /locus_tag="ACHKIK_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO9084678.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1107..1844
                     /gene="yjbG"
                     /locus_tag="ACHKIK_003579"
     CDS             1107..1844
                     /gene="yjbG"
                     /locus_tag="ACHKIK_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO9084679.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1844..3940
                     /gene="yjbH"
                     /locus_tag="ACHKIK_003580"
     CDS             1844..3940
                     /gene="yjbH"
                     /locus_tag="ACHKIK_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO9084680.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKVAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3987..4265)
                     /locus_tag="ACHKIK_003581"
     CDS             complement(3987..4265)
                     /locus_tag="ACHKIK_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084681.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4266..4409
                     /locus_tag="ACHKIK_003582"
     CDS             4266..4409
                     /locus_tag="ACHKIK_003582"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084682.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4535..4945
                     /gene="psiE"
                     /locus_tag="ACHKIK_003583"
     CDS             4535..4945
                     /gene="psiE"
                     /locus_tag="ACHKIK_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO9084683.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4989..6464)
                     /gene="xylE"
                     /locus_tag="ACHKIK_003584"
     CDS             complement(4989..6464)
                     /gene="xylE"
                     /locus_tag="ACHKIK_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO9084684.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLHTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDATWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNALAAQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVTHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6836..7726)
                     /gene="malG"
                     /locus_tag="ACHKIK_003585"
     CDS             complement(6836..7726)
                     /gene="malG"
                     /locus_tag="ACHKIK_003585"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO9084685.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7741..9285)
                     /gene="malF"
                     /locus_tag="ACHKIK_003586"
     CDS             complement(7741..9285)
                     /gene="malF"
                     /locus_tag="ACHKIK_003586"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO9084686.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTTQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9439..10629)
                     /gene="malE"
                     /locus_tag="ACHKIK_003587"
     CDS             complement(9439..10629)
                     /gene="malE"
                     /locus_tag="ACHKIK_003587"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO9084687.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10994..12109
                     /gene="malK"
                     /locus_tag="ACHKIK_003588"
     CDS             10994..12109
                     /gene="malK"
                     /locus_tag="ACHKIK_003588"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO9084688.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12181..13521
                     /gene="lamB"
                     /locus_tag="ACHKIK_003589"
     CDS             12181..13521
                     /gene="lamB"
                     /locus_tag="ACHKIK_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO9084689.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13764..14684
                     /gene="malM"
                     /locus_tag="ACHKIK_003590"
     CDS             13764..14684
                     /gene="malM"
                     /locus_tag="ACHKIK_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO9084690.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14913..15095
                     /locus_tag="ACHKIK_003591"
                     /pseudo
     CDS             14913..15095
                     /locus_tag="ACHKIK_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3763 a         3875 c         3963 g         3506 t
ORIGIN      
        1 aaaacgccat cgctaattcc ccgcgccgta attcgcatgc tttagttgtg tatactcgat
       61 cccgcccgaa atgtttttgg gtaaatctcc attcattcaa tgaagggaaa ttgttatgaa
      121 aaaagttctg tatggcattt ttgccatatc tgcgcttgcg gcgacttctg cgtgggctgc
      181 acctgtacag gtgggcgaag cggcagggtc ggcagcaacg tcggtttcgg cggggagttc
      241 ctccgctacc agcgtcagca ccgtaagctc ggcggtgggt gtcgcgctcg cggcaaccgg
      301 tggcggtgat ggttctaata ccgggaccac cacaaccacg accaccagta cccagtaata
      361 aagtatgtat ccccaaaata atttgagtca ttgcatctgt ggcttgaagt atgaagggat
      421 ttaccataac cacactccgg tgtggttatt ctgcccctct ggagaagagt cgtgaagcga
      481 cctgcactca ttcttatctg cctgctatta caggcctgtt cagccacgac taaagagctg
      541 ggcaattcac tgtgggacag tctgttcggc acgccaggcg tacagctgac ggacgatgat
      601 attcaaaata tgccctacgc cagccagtac atgcagctta atggcgggcc gcaattgttt
      661 gtggtgctgg ccttcgctga agacggacaa caaaaatggg tcactcagga tcaagccact
      721 cttgtcacac aacatggtcg tctggtgaag actttgcttg gcggcgacaa cctgattgaa
      781 gtgaataacc ttgccgccga cccgctgatt aaacccgcac aaattgttga tggtgcaaca
      841 tggacgcgca cgatgggctg gaccgagtac cagcaggtac gctacgccac cgcacgctca
      901 gtcttcaaat gggatggcac cgataccgtc aaagtcggca gcgatgaaac cctggttcgc
      961 gtgctggacg aagaagtctc caccgaccag gcgcgctggc ataaccgcta ctggatcgac
     1021 agcgaagggc aaattcgcca gtcggaacag tatctcggcg cggattattt cccggtgaaa
     1081 accactctca tcaaggcggc aaaacaatga ttaaacaaac ttttgtcgcg ttgcttttga
     1141 gtgtgggagc gtcatcggtc ttcgcggcag gaaccgtcaa ggtgttcagc aatggcagca
     1201 gtgaggccaa aacactaaca ggcgcagagc atttaatcga tctggtaggc caaccgcggc
     1261 tggcaaacag ctggtggccc ggtgcggtga ttagcgaaga gctggcaacg gcggcagcat
     1321 tgcgtcagca gcaggcgttg ctgacacggc tggcagaaca gggcgcagat tccagcaccg
     1381 acgatgccgc cgccattaac gccttacgcc agcaaatcca ggcattgaaa gtgacaggca
     1441 ggcaaaaaat caatcttgat cccgatatcg tccgcgttgc cgaacgcggt aacccgccgt
     1501 tgcagggcaa ctacacgctg tgggtcggac caccgccgtc cacggtcacg ttgttcgggc
     1561 ttatcagcca tcctggcaat cagccattca ctccaggtcg cgacgtggcg agctatctct
     1621 ccgggcagaa cctgcttagc ggtgcggatc gcagctacgc gtgggtcgtt tacccggacg
     1681 gacgcacgca aaaagcgccg gtggcttact ggaacaagcg tcacgtagag ccgatgcccg
     1741 gcagcattat ttatgttggc ctcgcggact ccgtctggag tgagacgcct gatgccctta
     1801 acgccgacat tcttcagact ctgacgcagc ggatacccca ataatgaaaa aaagacatct
     1861 gcttagctta ctggcgctgg gcattagcat ggcttgctac ggcgaaacat atcctgcgcc
     1921 cattggcccg tcgcagtcgg atttcggtgg cgtaggatta ttacaaactc ccaccgcgcg
     1981 tatggcacgg gaaggggagt tgagtctgaa ctatcgcgat aacgatcagt accgttatta
     2041 ctcagcttca gtgcaactct tcccgtggct ggaaacaacg ctgcgctaca ccgacgtgcg
     2101 cacccggcag tacagcagcg tcgaagcgtt ctctggcgat caaacgtata aagataaagc
     2161 cttcgatctc aaactgcgtt tgtgggaaga gagttattgg ctgccgcaag tggcggttgg
     2221 tgcgcgggat atcggcggta cggggctgtt tgatgcggaa tatcttgttg ccagcaaagc
     2281 ctgggggccg ttcgatttta cgctcggcct gggctgggga tatctgggca ccagcggtaa
     2341 tgtgaaaaat ccgctctgtt cagccagtga taaatattgc tatcgcgata acagctacaa
     2401 acaggcggga tctatcgacg gtagccagat gttccacggt cctgcctcac tgtttggcgg
     2461 cgtggaatac cagacgccct ggcaaccgct gcgcctgaaa ctggagtatg aaggcaataa
     2521 ttatcagcag gattttgccg ggaagctgga gcaaaaaagt aagtttaacg tcggtgcgat
     2581 ttatcgcgtt accgattggg ccgacgttaa ccttagctat gaacgtggca acacctttat
     2641 gtttggcgtt acgttgcgca ccaactttaa cgatctgcgc ccgtcttaca acgataacgc
     2701 ccgcccgcaa tatcaaccgc agccgcagga tgccattttg cagcattcgg tggtggcgaa
     2761 tcagttaacg ttgctgaaat acaatgccgg acttgccgat ccgcagatcc aggcgaaagg
     2821 cgatacgctg tatgttaccg gcgagcaggt gaaatatcgt gattcgcgcg aagggatcat
     2881 ccgtgcgaat cggatcgtga tgaacgatct gccggatggg atcaaaacga tccgcattac
     2941 agaaaatcgc cttaacatgc cgcaggtgac gacggaaacc gatgtcgcca gcttgaaaaa
     3001 tcatctcgcc ggggagccgc tgggccatga aacgaaggtg gcgcaaaaac gcgtcgagcc
     3061 agtggttccg aagtccaccg agcagggatg gtatatcgac aaatcacgct ttgatttcca
     3121 tatcgatccg gtgctgaacc agtcggtcgg tggcccggaa aacttttaca tgtatcagct
     3181 gggcgtgatg ggaacggcag atttgtggct gacggaccat ctgctgacca ccggcagcct
     3241 gtttgcaaat cttgccaaca actacgacaa gtttaactac accaatccgc cgcaggactc
     3301 gcacttaccg cgcgttcgta cccatgtgcg cgagtatgtg cagaacgatg tctatgtgaa
     3361 taacctgcaa gccaactact tccagcatct gggcaacggc ttctacggtc aggtctatgg
     3421 aggttatctc gaaaccatgt ttggcggtgc cggggcagaa gtgttgtatc gtccgctgga
     3481 cagcaactgg gcgtttggtc tggatgccaa ctacgttaaa cagcgcgact ggcgtagtgc
     3541 aaaagatatg atgaaattca ccgactacag cgtgaaaacc ggacatctga ccgcctactg
     3601 gacgccatct ttcgcccagg acgtgttggt gaaagcgagc gtcgggcagt atctggcagg
     3661 ggataaaggc ggcacgctgg agatcgccaa acgctttgat agcggcgtgg tggttggcgg
     3721 ctatgccacg atcacgaatg tttcgaaaga ggagtacggc gaaggggact tcaccaaagg
     3781 cgtgtatgtc tctgtgccgt tggatctctt ctcgtctggc ccgacacgca gccgtgcggc
     3841 gattggctgg acgccgctga cgcgtgacgg tggtcagcaa cttgggcgta agttccagtt
     3901 gtatgacatg accagcgacc gtagcgtcaa tttccgctaa gtcatgggaa aggtgccagt
     3961 tttcgcaatc actggcaccc tcattcttaa tggcctgttg agccatcagg aacgcagttc
     4021 acccccggtt tgcgatctgg tgtgtaaagg cgaaaacatt ctgtcttcct gtccgtcgat
     4081 atcggttgca gatctggttg cctgagcgac tgggccatca ttgggctgga gacattgctt
     4141 tgcggtaggt cggctttatc agcagtaccg agcggaccag cataagcagg aagaacagag
     4201 actgataaca tcaaagcagc aaaataaggc ttcattttta ccacctttat caggttacgt
     4261 ttcatttgtt ccagaggaac attgtcgatt tttcgcgcat tgctggtggc tgggaatcac
     4321 ctgaatgggt gatttttgaa ttaccggctt tggtgcggtt tgtcttgccg gatgcgccgc
     4381 caggcgcggc ttatccggcc tacgggtagg tatatccggc tttggtggag gcgcgctcca
     4441 aatccaggtt gaacaaaaca tacacaaaaa atatagatct ccgtcacatt tttgcgttat
     4501 acaggaagct cgccactgtg aaggaggtac tgctatgacg tcactctctc gtccgcgcgt
     4561 ggagtttatc tccaccattt tgcagaccgt actcaatctt ggcctgttgt gcctcggcct
     4621 gattttggtt gtcttcctcg gcaaagaaac ggtgcatctg gctgatgtgc tgttcgcgcc
     4681 agaacaaacc agcaaatatg agctggtaga agggctggtg gtttactttc tctatttcga
     4741 atttatcgcg ctgattgtga agtactttca gtccggtttt cacttcccgc tacgttactt
     4801 tgtctatatt gggatcaccg ccattgtgcg cttgatcatc gtcgatcata aatcgccact
     4861 ggatgtattg atctactcgg cagcgatcct gttactggtg atcaccctgt ggctgtgtaa
     4921 ttcgaaacgg ctgaagcggg agtaaaaaga cagcacgccg aactggcgcg gcgtgctgga
     4981 caggaagatt acagtgtagc agtttgttgt gttttcttcg tttccggttc ccagagggct
     5041 tccagctcct caagggtttt acctttggtt tccgggacaa atttccacat aaatagtgct
     5101 gccagaacgc ccatacaacc gtaaatccag taggagaaac cgttgtggaa atgggtcacc
     5161 agccaggagt ttttgtccat catcgggaag gtccaggaga cgaagtagtt cgccagccac
     5221 tgggccgcca ccgcgattgc cagcgcttta ccacgaatag cattcgggaa gatttccgac
     5281 agcagtaccc agcataccgg accccaggac atggcaaagg cagcaacata gaacagcatc
     5341 gacagtagcg ccacaatacc cggtgcctga gtgtaaaacg cggtaccgag gctaaacata
     5401 ccgattgcca ttccgagcgc gccgataatt tgcagtggct tacgaccaaa tttatccacc
     5461 gtcataattg ccagaacggt gaaggtgagg ttgataactc cgacaataat ggtctgcaac
     5521 agcgcgatat ccgtgctggc ccccagcgtt ttgaacactt ccggcgcgta gtacaacacc
     5581 acattgatgc cgacaaattg ctggaagatg gagagcataa cgccgataac aatcacgccc
     5641 acgccgaaca tcagcagacg accaccggtt ttgcgtccat gatccaggga gtgtttaatt
     5701 tcctgcaccg cctgggctgc caaagcgttg cccataattt tgcgcaggat gccttccgct
     5761 tgttcttgct taccgcgtga catcagccag cgagggcttt ccggcacggt ataaagcagc
     5821 attaagaaca gcagcgcagg gatacattcc gaagcaaaca tataacgcca gccgtcagta
     5881 ttcagccagg tggcatcgcc ggaacgggca ataaaatagt ttacgcagta aactaaaagt
     5941 tggccgaaaa taatcgcaaa ctggttaaaa gagaccagct tcccacgaat atgtgcagga
     6001 gccagttccg caatatacat tggtgagagc attgaggcta aaccaacacc aataccgcca
     6061 ataatgcgat aaataacaaa ttccgggaca taacctgcca gataaacagg cactgtgttg
     6121 tccgggttta tagaggtaaa accaagttct ggccaggcag aacctacacc agaaataaaa
     6181 aacaggacag cagcaatctt aagtgaatca cgacgaccga agcggttact gcaataacca
     6241 ccgagggcac cgccgatgat gcaaccaatc agagcgctgg ctacgcaaaa ccctaacagg
     6301 gagttggcag cggattcact taagttttgt ggagcaacaa agacggtatg gagtgactca
     6361 acagtaccgg aaataacggc ggtgtcgtag ccaaataata aaccacctaa tgtagcgact
     6421 aaggtaatcg aaaatatata actggaatta tactgggtat tcattcagac ctgccttaga
     6481 ccattctgat cttttatcag tgcattgatg ttggaattgt caggcagtta aggcaattat
     6541 cattttttac aatgcgttta cgtaatgtct tttctgtgat cttaattgtg ataattatct
     6601 aaaattgatg aaaacaatac tattgccgtg actcagagca cgaaagagaa ttatcgtaag
     6661 tgggaaaaca aataacgtaa aaataataag ctctatgatg ggaatattaa ccggcgaacg
     6721 attcagattg cagacgaaag aaaaaaaggc gctccgtgga gcgccgaata acagtcacaa
     6781 gttgggataa cgtaagttga gggtgcagcg gcataacatt ggcagaacaa catctttaac
     6841 ctttcacacc acctgccgtc aggccgttga ccagccagcg ttgagccagc aagaagacga
     6901 tggtgatcgg taatgcagac atcacggcag cggcggcaaa gtcaccccac aggtagtttt
     6961 gcgggttgag gtattgctgc atccccacgg ccagggtgta gctgtttacg tcacgcagta
     7021 acagcgacgc gaccggaact tcagtaatgg cagcgataaa cgacaagatg aataccaccg
     7081 ccagaatcgg tactgacagc ggcaacagga caaggcggaa ggcctgccac ggtgtcgcac
     7141 catccagcgc agccgcttct tccagcgaac tatcgatggt ttcgaaatag cctttgatgg
     7201 tccagacatg cagcgcaatc ccacccagat acgcgaaaat tacgccgccg tgagtattca
     7261 ggccaatgaa tggaatgtac tcacccagac gatcaaacaa cgcatacaac gcgaccagtg
     7321 aaagtactgc cgggaacatc tggaaaatca gcattccttt cagcagcgtc gctttgcctg
     7381 gaaagcgcat acgggcgaaa gcgtaggcgc aggtggtaga gagcgccaca atgccaatcg
     7441 cggaaatccc ggcgaccttt accgagttcc acagccacag cagtaccggg aatggcggtg
     7501 gcgtaatgcg accatcagcc tgttcaacgc taaaacctaa cgccagtttc cagtgatccc
     7561 aggagatttg ctccgggatc aggctaccgg tcgcgaagtt tccctgacgc agcgagatag
     7621 cgacgaccat cagcagcggg aacataatcg ccgcgataaa aagtagcagt agcaggtgag
     7681 taataaacaa acgtgctttt tgcgatttcg gttggaccat tgccattttt gttatctccc
     7741 ttaatcaaac ttcattcgcg tggctttcag gttcactatc gccagcgcac ccaccagcag
     7801 gaagatcagc gtggcaattg ctgctgccag accgaagtcc tgacccccgc cgccttcaaa
     7861 agcgatgcgg taggtgtagt taacaagcag gtcggtataa ccggctggcg tggtcgtgcc
     7921 aagacgatcc gggccgccgt tggttaacag ttgaatcagc acgaagttgt taaagttaaa
     7981 ggcgaagctg gcgatcatca gcggcgtcag cggtttaatc agcagcggca gcgtaatctt
     8041 aaagaagttc tggaacggac ctgcgccatc cattgctgag gcttcataca aatcgtccgg
     8101 aatcgctttc agcaagccca tgcagaggat catcatgtac ggataaccca gccaggtatt
     8161 gacgataatc agcatcgtgc gggcggtggt cggatcgctg aaccaggcgg gcttcacgcc
     8221 aaacagcgcg ctcaacatca tgttgatttc accgaagctc tggttaaaca accctttgaa
     8281 aatcaagatt gaaatgaacg atggcaccgc gtagggcaga atcagcagga cgcgatagac
     8341 cgctttgccg cgcaacgctt cccactgcac cagacacgcc agaaccatgc cgaccgccac
     8401 cgttaaaaag acagtgatca gcgagaacac cacggtccag acgaaaatgg cgaggaacgg
     8461 tttctgaatg ccttcgtcgg tgaagacgcg ggtaaagttt ttccagccag tagtcacggt
     8521 gtaaccgggg cttagctttt catcgcccca gttgccgtcg gcggtaatgg actggtaaaa
     8581 gccaatttgg ttattcggac gatatttcac gccgctctga ttattcgtca acgtgccgtc
     8641 accgtcgagt gtgtagagcg gctgcgtgcc agaaaactgg cgcagggagc tcatcatcac
     8701 tttgttgcca tccggcagaa tggcggtaat gtcactcagc gcctgacgat tctgggtaat
     8761 cacgcgcaga ttcgcgcgtt cgccttcggg ctgggtggtc gtttctttca gttgcagttt
     8821 ttgctcgccg ccaaatttaa aagcgtcgga gaggtaattt ttgccggttt cgccgtcgct
     8881 gagggctagt tgccactcat cgcccgccgg gtaaagacca aaattatagg ttttgcctgc
     8941 ttgccaggag cgatctagca acacttcctg cgcacgttca aaagtcagct ggttagtgct
     9001 gctgtagttg gtgaaggcaa tggcgatggt gcagaccaga gggaagagga cgaataatcc
     9061 cattccagcc attcccgggt aaacatagcg ccaggcgtag gctttacgat tggcgaaaat
     9121 atacagcccc gctgaactca atatcagcgt ggtaatggcg aacaggtatt ccccttgtgc
     9181 gtacattaaa acaacaaggt aacccaccag caggccgagc agacctaaca ctgaccattt
     9241 cagcgcgtcg ctttgccacc aatgtttctt tttaatgaca tccatggggt tcttcctcat
     9301 tccaggacgg ataaggcttt cacgccttat ccgacaacaa ctgcctgatg cgacgctgac
     9361 gcgtcttatc aggcctacat acgtttcggt tttgtaggcc ggacaaggcg ttcacgccgc
     9421 atccggcatt tcacagcatt acttggtgat acgagtctgc gcgtctttca gggcttcatc
     9481 gacagtctga cgaccgctgg cggcgttgat caccgcagta cgcacggcat accagaaagc
     9541 ggacatctgc gggatgttcg gcatgatttc acctttctgg gcgttttcca tggtggctgc
     9601 aatacgtgga tctttcgcca actcttcctc gtaagacttc agcgctacgg cacccagcgg
     9661 tttgtcttta ttaaccgctt ccagaccttc gtcagtcagc agatagtttt cgaggaactc
     9721 tttcgccagc tctttgttcg gactggcggc gttaataccg gcgctcagca cgccaacgaa
     9781 cggtttagat ggttgaccct tgaaggtcgg cagtaccgtt acaccataat tcactttgct
     9841 ggtgtcgatg ttggaccatg cccacgggcc gttgatggtc atcgctgttt cgcctttatt
     9901 aaaggcagct tctgcgatgg agtaatcggt gtctgcattc atgtgtttgt ttttaatcag
     9961 gtcaaccagg aaggtcagac ccgctttcgc gccagcgtta tccacgccca cgtctttaat
    10021 gtcgtatttg ccattttcat acttgaacgc ataacccccg tcagcagcaa tcagcggcca
    10081 ggtgaagtac ggttcttgca ggttgaacat cagcgcgctc ttacctttcg ctttcagttc
    10141 tttatccagc gccgggatct cttcccaggt ttttggtggg ttcggcagca gatctttgtt
    10201 ataaatcagc gataacgctt caacagcgat cgggtaagca atcagcttgc cgttgtaacg
    10261 tacggcatcc caggtaaacg gatacagctt gtcctggaac gctttgtccg gggtgatttc
    10321 agccaacagg ccagattgag cgtagccacc aaaacggtcg tgcgcccaga agataatgtc
    10381 agggccatcg ccagttgccg caacctgagg gaatttctct tccagtttat ccggatgctc
    10441 aacggtgact ttaattccgg tatctttctc gaatttctta ccgacttcag cgagaccgtt
    10501 atagccttta tcgccgttaa tccagattac cagtttacct tcttcgattt tggcgagagc
    10561 cgaggcggaa aacatcatcg tcgttaatgc ggataatgcg aggatgcgtg cacctgtttt
    10621 tattttcata atctatggtc cttgttggtg aagtgctcgt gaaaacacct aaacggactc
    10681 tagtttcttt atacggcaac ctctttccat cctccttgcc cctacgcccc accgttgctt
    10741 tgtgtgatct ctgttacaga attggcggta atgtggagat gcgcacataa aatcgccacg
    10801 atttttgcaa gcaacatcac gaaattcctt acatgacctc ggtttagttc acagaagccg
    10861 tgttctcatc ctcccgcctc ctcccccata aaaaagccag ggggtggagg atttaagcca
    10921 tctcctgatg acgcatagtc agcccatcat gaatgttgct gtcgatgaca ggttgttaca
    10981 aagggagaag ggcatggcga gcgtacagct gcaaaatgta acgaaagcct ggggcgaggt
    11041 cgtggtatcg aaagatatca atctcgatat ccatgaaggt gaattcgtgg tgtttgtcgg
    11101 accgtctggc tgcggtaaat cgactttact gcgcatgatt gccgggcttg agacgatcac
    11161 cagcggcgac ctgttcatcg gtgagaaacg gatgaatgac actccgccag cagaacgcgg
    11221 cgttggtatg gtgtttcagt cttacgcgct ctatccccac ctgtcagtag cagaaaacat
    11281 gtcatttggc ctgaaactgg ctggcgcaaa aaaagaggtg attaaccaac gcgttaacca
    11341 ggtggcggaa gtgctgcaac tggcgcattt gctggatcgc aaaccgaaag cgctctctgg
    11401 cggtcagcgt cagcgtgtgg cgattggccg tacgctggtg gccgagccaa gcgtattttt
    11461 gctcgatgaa ccgctctcca acctcgatgc tgcactgcgt gtgcaaatgc gtatcgaaat
    11521 ctcccgtctg cataaacgcc tgggccgcac aatgatttac gtcacccacg atcaggtcga
    11581 agcgatgacg ctggccgaca aaatcgtggt gctggacgca ggtcgcgtgg cgcaggttgg
    11641 gaaaccgctg gagctgtacc actatccggc agaccgtttt gtcgccggat ttatcggttc
    11701 gccaaagatg aacttcctgc cggtaaaagt gaccgccacc gcaatcgatc aagtgcaggt
    11761 ggagctgccg atgccaaatc gtcagcaagt ctggctgcca gttgaaagcc gtgatgtcca
    11821 ggttggagcc aatatgtcgc tgggtattcg cccggaacat ctactgccga gtgatatcgc
    11881 tgacgtcatc cttgagggtg aagttcaggt cgtcgagcaa ctcggcaacg aaacccaaat
    11941 ccatatccag atcccttcca ttcgtcaaaa cctggtgtac cgccagaacg acgtggtgtt
    12001 ggtagaagaa ggtgccacat tcgctatcgg cctgccgcca gagcgttgcc atctgttccg
    12061 tgaggatggc actgcatgtc gtcgactgca taaggagccg ggcgtttaag caccccacaa
    12121 aacacacaaa gcctgtcaca ggtgatgtga aaaaagaaaa gcaatgactc aggagataga
    12181 atgatgatta ctctgcgcaa acttcctctg gcggttgccg tcgcagcggg cgtaatgtct
    12241 gctcaggcaa tggctgttga tttccacggc tatgcacgtt ccggtattgg ctggacaggt
    12301 agcggcggtg aacaacagtg tttccagact accggtgctc aaagtaaata ccgtcttggc
    12361 aacgaatgtg aaacttatgc tgaattaaaa ttgggtcagg aagtgtggaa agagggcgat
    12421 aagagcttct atttcgacac taacgtggcc tattccgtcg cgcaacagaa tgactgggaa
    12481 gctaccgatc cggccttccg tgaagcaaac gtgcagggta aaaacctgat cgaatggctg
    12541 ccaggttcca ccatctgggc aggtaagcgc ttctaccaac gtcatgacgt tcatatgatc
    12601 gacttctact actgggatat ttctggtcct ggtgccggtc tggaaaacat cgatgttggc
    12661 ttcggtaaac tctctctggc agcaacccgc tcctctgaag ctggtggttc ttcctctttt
    12721 gccagcaaca atatttatga ctataccaac gaaaccgcga acgacgtttt cgatgtgcgt
    12781 ttagcgcaga tggaaatcaa cccgggcggc acattagaac tgggtgtcga ctacggtcgt
    12841 gccaacctgc gtgataacta tcgtctggtt gatggcgcat cgaaagacgg ctggttattc
    12901 actgctgaac atactcagag tgtcctgaag ggctttaaca agtttgttgt tcagtacgct
    12961 actgactcga tgacctcgca gggtaaaggt ctgtcgcagg gttctggcgt cgcgtttgat
    13021 aacgaaaaat ttgcctacaa tatcaacaac aacggtcaca tgctgcgtat cctcgaccac
    13081 ggtgcgatct ccatgggcga caactgggac atgatgtacg tgggtatgta ccaggatatc
    13141 aactgggata acgacaacgg caccaagtgg tggaccgttg gtattcgccc gatgtacaag
    13201 tggacgccaa tcatgagcac cgtgatggaa atcggctacg acaacgtcga atcccagcgc
    13261 accggcgaca agaacaatca gtacaaaatt accctcgcac aacaatggca ggctggcgac
    13321 agcatctggt cacgcccggc tattcgtgtc ttcgcaacct acgccaagtg ggatgagaaa
    13381 tggggttatg actacaacgg cgatagcaag gttaacccga actacggcaa agccgttcct
    13441 gctgatttca acggcggcag cttcggtcgt ggcgacagcg acgagtggac cttcggtgcc
    13501 cagatggaaa tctggtggta atagcaaaac ctgggccgga taaggcgttt acgccgcatt
    13561 cggcaaccaa cgcctgatgc gacgcttgcg cgtcttatca ggccgacaac ggctgtcaaa
    13621 tgtaggccgg ataaggcgtt tacgccgcat ccggcataaa aacaggttgt cattatctga
    13681 aaggggcgaa agcccctctg attatcgggt ttagcgcgct attgcctggc taccgctgag
    13741 ctccagattt tgaggtgaaa acaatgaaaa tgaataaaag tctcatcgcc ctctgtttat
    13801 cagcagggtt actggcaagc gcgcctggaa tcagccttgc cgatgttaac tacgtaccgc
    13861 aaaacaccag cgacgcgcca gccattccat ctgctgcgct gcaacaactc acctggacac
    13921 cggtcgatca atctaaaacc cagaccaccc aactggcgac cggcggccaa caactgaacg
    13981 ttcccggcat cagtggtccg gttgctgcgt acagcgtccc ggcaaacatt ggcgaactga
    14041 ccctgacgct gaccagcgaa gtgaacaaac aaaccagcgt ttttgcgccg aacgtgctga
    14101 ttcttgatca gaacatgacc ccatcagcct tcttccccag cagttatttc acctaccagg
    14161 aaccaggcgt gatgagtgca gatcggctgg aaggcgttat gcgcctgaca ccggcgttgg
    14221 ggcagcaaaa actttatgtt ctggtcttta ccacggaaaa agatctccag cagacgaccc
    14281 aactgctcga cccggctaaa gcctatgcca agggcgtcgg taactcgatc ccggatatcc
    14341 ccgatccggt tgctcgtcat accaccgatg gcttactgaa actgaaagtg aaaacgaact
    14401 ccagctccag cgtgttggta ggacctttat ttggttcttc cgctccagct ccggttacgg
    14461 taggtaacac ggcggcacca gctgtggctg cacccgctcc ggcaccggtg aagaaaagcg
    14521 agccgatgct caacgacacg gaaagttatt ttaataccgc gatcaaaaac gctgtcgcga
    14581 aaggtgatgt tgataaggcg ttaaaactgc ttgatgaagc tgaacgcctg ggatcgacat
    14641 ctgcccgttc cacctttatc agcagtgtaa aaggcaaggg gtaattacgc cccacagtgc
    14701 tgattttgca acaactggtg cgtctcctgg cgcacctttt tttatgcttc cttcctggga
    14761 tatgagcgat tttttatagt aactcacttc ttcttcacta agaatatcca ttatctcaat
    14821 gccttatcag agattctttt cctttcgccg gtagtgtctg gacattcagg ctacttttcc
    14881 aggttatttt atttctgtta tgcagaggtt ttatgataag tcatatccta aattctggcg
    14941 gcaataactc tttgatgaaa catgatgtgg tgcaaggaaa taatatagta gatcttgatt
    15001 tactacgtaa tttcaatggg gtgccaggtt taaacagaga taactttatt tatatcagca
    15061 atattttttc aaatataaaa caacggaacg aaaaaatcat gcaataa
//
LOCUS       DAZGJW010000115        15024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZGJW010000115 DAZGJW010000000
VERSION     DAZGJW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.115"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(71..146)
                     /locus_tag="ACHKIK_003592"
     tRNA            complement(71..146)
                     /locus_tag="ACHKIK_003592"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Phe,seq:gaa)
     gene            complement(252..959)
                     /gene="yqgA"
                     /locus_tag="ACHKIK_003593"
     CDS             complement(252..959)
                     /gene="yqgA"
                     /locus_tag="ACHKIK_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO9084691.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1112..1195
                     /gene="yqgH"
                     /locus_tag="ACHKIK_003594"
     CDS             1112..1195
                     /gene="yqgH"
                     /locus_tag="ACHKIK_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFO9084692.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            1357..3492
                     /gene="speC"
                     /locus_tag="ACHKIK_003595"
     CDS             1357..3492
                     /gene="speC"
                     /locus_tag="ACHKIK_003595"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO9084693.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3542..4798)
                     /gene="nupG"
                     /locus_tag="ACHKIK_003596"
     CDS             complement(3542..4798)
                     /gene="nupG"
                     /locus_tag="ACHKIK_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO9084694.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5000..6079)
                     /gene="mltC"
                     /locus_tag="ACHKIK_003597"
     CDS             complement(5000..6079)
                     /gene="mltC"
                     /locus_tag="ACHKIK_003597"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO9084695.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6144..6419)
                     /gene="yggX"
                     /locus_tag="ACHKIK_003598"
     CDS             complement(6144..6419)
                     /gene="yggX"
                     /locus_tag="ACHKIK_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO9084696.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6447..7499)
                     /gene="mutY"
                     /locus_tag="ACHKIK_003599"
     CDS             complement(6447..7499)
                     /gene="mutY"
                     /locus_tag="ACHKIK_003599"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO9084697.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7660..8379
                     /gene="trmB"
                     /locus_tag="ACHKIK_003600"
     CDS             7660..8379
                     /gene="trmB"
                     /locus_tag="ACHKIK_003600"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO9084698.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8379..8705
                     /gene="yggL"
                     /locus_tag="ACHKIK_003601"
     CDS             8379..8705
                     /gene="yggL"
                     /locus_tag="ACHKIK_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO9084699.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            8889..9608
                     /gene="yggN"
                     /locus_tag="ACHKIK_003602"
     CDS             8889..9608
                     /gene="yggN"
                     /locus_tag="ACHKIK_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO9084700.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9784..10830
                     /gene="ansB"
                     /locus_tag="ACHKIK_003603"
     CDS             9784..10830
                     /gene="ansB"
                     /locus_tag="ACHKIK_003603"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO9084701.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVAAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            10947..11954
                     /gene="yggM"
                     /locus_tag="ACHKIK_003604"
     CDS             10947..11954
                     /gene="yggM"
                     /locus_tag="ACHKIK_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO9084702.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQAFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(12010..13311)
                     /locus_tag="ACHKIK_003605"
     CDS             complement(12010..13311)
                     /locus_tag="ACHKIK_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO9084703.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLVYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(13311..13814)
                     /locus_tag="ACHKIK_003606"
     CDS             complement(13311..13814)
                     /locus_tag="ACHKIK_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO9084704.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(13859..14845)
                     /locus_tag="ACHKIK_003607"
     CDS             complement(13859..14845)
                     /locus_tag="ACHKIK_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFO9084705.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         3990 a         3593 c         3791 g         3650 t
ORIGIN      
        1 ggcaggacgc ttaaatcgtg gcgtcctgaa acgaaaaacg gacctccgtg gaggtccgtt
       61 tatatgaatt tggtgcccgg actcggaatc gaaccaagga cacggggatt ttcaatcccc
      121 tgctctaccg actgagctat ccgggcaacg gggcgcatta aacctgattc gcctcgtctc
      181 gtcaatcaaa tttcttcaat ttactgcaga ctgcacaatc tatcatcact ttgccgctat
      241 tgcacgcatt gtcaggcaaa ccaggcggtc caggcggcgg aaagcggcat tgccagaagg
      301 agtgctggaa gcatgttgac caccgggaac attttaatgc cacagatgcg taatccggtt
      361 gccagcagca ataaaccgcc tacggcgctg aagtctgcca tcatcgacgg tgtggtcagc
      421 ggtaatatca gcgcggcagc ccacgccagc gttaactgga tgatcagtaa tgggatacta
      481 attaccgaca ccgcaatacc cagcgagcag gcgaagatca tcgccgtaaa gaaatcaaga
      541 aatgacttgg cgattaaaat actcggatcg ccggtcatcc cttcgttcat cgccccgaag
      601 atcccggtgc cgctggcgca aaacaggaca ataatcgcga cataattctg aataaaagat
      661 tcatgcgctg gcttcttacg tgagtgacga aacagatttt gtgctttggc gactgctgta
      721 ttgacacctt tctccagcaa acaaatttcg ccaattagag cgccaagtag cgtagctaaa
      781 accatcgctg gaaggttggc gcatttcaca accagtaaaa taccaatccc cagcgatgcc
      841 agaccaaaaa tagatgtcat ggagacgcgg atacgttccg gtaagcgttg gctgagcagt
      901 gcgccgagaa cgccacccag taagactgca ctagcgttga taaaagggcc gatgaccaca
      961 agagttcctg ttagctgtat tgtttgattt tcttattatg aactttttgc cttgttgatg
     1021 gcttgtctgg tcgcaaagtc ctggcttgca cgctttagcg aaaggtgcca tgattgcgcg
     1081 aattttctcc tctctgtacg gagtttgccc gatgcacgcc atctccttac attctctcgc
     1141 ttatcgccgt ttcgcgcgaa acgtttccct ttctatgtta ctgctcatgc ggtgaagtta
     1201 acgcacgctc actgcaggac aacagtaaaa tcagagcgtt tctgctttta ctgatgtctg
     1261 gcggtcggag ctggtgacca gtttgaccca tatctcatgg ggcagggttt tccaccttgt
     1321 ccggtattct tacttccccg aaacgggttt gcgcttatga aatcaatgaa tattgccgcc
     1381 agtagtgaac tggtatcccg actttcttct catcgtcgcg tggtggcgtt gggagatact
     1441 gattttacgg acgtcgcggc agtcgtcatt accgctgcgg atagtcgcag tggcattctt
     1501 gcgttgctta agcgcaccgg ttttcatcta ccggtgtttt tgtattccga acatgctgtt
     1561 gaattacctg cgggcgttac ggcggtaatc aacggcaacg agcagcagtg gctggagctg
     1621 gaatccgcag cctgtcagta tgaagagaat ttgctgccac cgttttatga cacgctgacg
     1681 cagtacgttg agatgggcaa cagcaccttt gcttgccctg gacatcaaca tggtgcgttt
     1741 tttaaaaagc atcctgccgg acgccatttt tacgatttct ttggtgagaa cgtctttcgc
     1801 gccgatatgt gtaacgctga cgtaaaattg ggcgatctgc ttattcatga aggatcggcg
     1861 aaagatgcgc agaaattcgc agccaaagtc tttcatgccg ataaaaccta ttttgtgctg
     1921 aacggcacat cggcagcgaa taaagtggtg acgaatgcgc tgttaacgcg tggcgatctg
     1981 gtgctcttcg accgtaacaa ccataagtcg aatcatcacg gcgcgctgat tcaggcgggg
     2041 gcgacgccgg tctatctgga agcttcacgc aacccgtttg gtttcattgg cggtattgat
     2101 gcgcactgtt ttaatgaaga gtatctgcgc cagcaaattc gcgacgttgc gccagaaaaa
     2161 gccgagctgc cgcgcccgtt tcgcctggcg attattcagc tgggaaccta tgacggaact
     2221 gtctataacg cccgtcaggt gatcgatacc gttgggcatc tgtgtgatta cattctgttt
     2281 gattccgcgt gggtcggtta cgaacaattt atcccgatga tggcggatag ctcgccgctg
     2341 ctgttagaac ttaacgaaaa cgatccgggg atttttgtga cccagtcggt gcacaaacag
     2401 caggctggat tctcacagac gtcgcagatc cataaaaaag ataaccatat ccgcggacag
     2461 gcgcgttttt gcccgcataa gcggttgaat aacgccttta tgctccatgc ttctaccagc
     2521 cctttctatc cgctgtttgc tgcactggat gttaacgcca aaattcatga aggggagagt
     2581 gggcgtcggc tgtgggctga gtgcgttgag ttgggtattg agtcgcgcaa ggctattctt
     2641 gcgcgctgta agctgttccg cccgtttatc ccgcccgttg ttgatggcaa attgtggcag
     2701 gattatccga catctgtgtt agccagcgac cgccgttttt tcagttttga gccgggggcg
     2761 aagtggcacg gctttgaagg atatgccgcg gatcagtatt ttgttgatcc gtgcaagctg
     2821 ttactcacta caccaggtat caatgccgaa actggcgaat atagcgactt tggcgttccg
     2881 gcgacgattc tggcgcacta tctgcgtgag aacggcattg tgccggagaa gtgcgatctc
     2941 aactccattc tgttcttatt aactccggcg gaaagccacg agaagctggc acaactggtg
     3001 gcgatgctgg cgcaatttga acagcatatt gaggatgact cgccgctggc tgaggtgttg
     3061 ccgagcgttt ataacaaata tccggtgcgc tatcgcgact acaccctgcg ccagttgtgt
     3121 caggagatgc acgatctgta tgtcagtttc gacgtcaaag acctacaaaa agcgatgttc
     3181 cgccagcaga gtttcccgtc agtggtgatg aacccccagg atgcgcatag cgcttatatt
     3241 cgcggtgaag tggagttggt gcggattcgt gatgccgaag ggcgaattgc ggcagaaggg
     3301 gcgttgcctt atccacctgg cgtgctttgc gtggtacccg gggaagtctg gggtggggcg
     3361 gttcaacgtt atttccttgc actggaagaa ggggtgaatt tgttgccggg attttcgccg
     3421 gagctgcaag gtgtttatag cgaaaccgat gcggatggca tgaaacggtt gtacggttat
     3481 gtgttgaagt aagaataaaa aaaacgggtc accttctggc gacccgtttt tctttgcgta
     3541 attagtggct aaccgtctgt gtgcctgtcg ggacacgaac gtgtttatat ttgaacatcg
     3601 ccatgaacgc gaaggccaga accacggagt aaccagcgaa aatcaaccat acggtctgcc
     3661 agtcggtaat gccgttttgg gtgtacatct caacaacttt accgctcacg atgccgccga
     3721 ggatacagcc gaagccgtta gtcatcatca ggaacatccc ttgtgcactg gcgcgaattg
     3781 ccgggctaac ttctttttcg acaaacaccg aaccagagat gttgaagaag tcgaatgcgc
     3841 aaccgtaaac gatcatcgac agtaccagca gtacagtacc gaacggagtc gggtcgccgt
     3901 aagcaaacag cgcaaaacgc aggatccacg ccacaatact gatcatcatt acgttcttaa
     3961 taccgtagcg gcttaagaag aacgggatgg tcagaatgaa cagggtttca gagatctgcg
     4021 aaatcgacat gatgattgac gcatgctgca caataaagct gctggcaaac atcggatctt
     4081 tgtcgaagct gtgcaggaag gtattaccga acatattggt aatctgcagt tccgcgccca
     4141 gcagcattga gaagataaag aagattgcca tacgcttgtt tttaaacagc gcgaatgcat
     4201 cgaggcccag cagggttgtc cagctctgat tcgcttgctg tttagcaacc ggaatatgcg
     4261 gcagagtcag ggtaaacaga accagaatgg cggaaagtgc tgcgccaata tacagctgca
     4321 tgtggcttaa ttcgaagcca gacaggctca ccacccacat tgccatgata aagccgatgg
     4381 tgccccagat acggattggc gggaagtcag taacgatatc catcccggca ttttgcaggc
     4441 gatagtaaga gatggtgttg attaacccaa gcgttggcat ataagcaaac gagttaatca
     4501 atatcacaag gaacatcgcc tccggtgtcg tgacctgtgc cgccatgaac agcgtgatag
     4561 cgccaatggt gtggcaaatg gcatataccc atttcgcact taaccatttg tcggccacaa
     4621 tccccagcag cgcaggcata aagaccgcgg cgatacccag tgagctataa actgcgccaa
     4681 tagaagcacc gtcaaacttc agggtaacaa acatatagga gccgagggtc gtcagccaac
     4741 ttccccacag acagaactgc agaaaagaga ggattttcag ctgcagctta agattcatgt
     4801 taatttcctc acatcgtgat gcggatgagt gtttgaaaaa gcacatttga ccgtgcgaga
     4861 tgatgggatt ctatcaactt cgcggactgg ttttttgtta cctgtggcaa ataattgcaa
     4921 acattcccaa tttgcaaaac gaaattgtga tgtggataac atttttgccc ctgagcatcg
     4981 tcaggggcgg ttaatggaat tatcggcggc ggtaggattt ttgcgcggta ttcactttat
     5041 aaagataacg gcgagattcc gcagagggat ggcgggtcgt cagcgtctga taaacatcgc
     5101 ccggcgtcat ggtgttaata atattggcag cctgaatctt atcattcgaa aagactcgca
     5161 gcacgctgcc tgcgccgccg ttataggcgg tgatgacggc ataacgccgc gatgttgggt
     5221 tatcaattcc gccgagataa acattgttca gcatcgccag atacgcggtg ccggtatcaa
     5281 tattgctggc aggatcaaac aagaaactgc ggctcggcgt gccggatttc ccctgcgagc
     5341 ggaacacatc tttcccggca gtatgttgta ccacctgcat taatcccagc gcatcggaac
     5401 ggctgaccgc atatgggtta aaggaagatt cggtctgcat aatcgccaga atcagcgact
     5461 catcaacgcc atatttccgt gacgcctggc ggaccatgcc gagatatttg tgcgcacgtt
     5521 tatcaaggtg gttcggcacc atgttaatgg taacgctgta gatgatacgc agcccgttgc
     5581 tgcggctctt cagacggttt ttcagcagat aatccgcgaa gttgctcgcg cgaccttccc
     5641 agcgaatcgg ctgcccggtg ttgtccacca cctgaccgta aaggaaaggt tctttcgaaa
     5701 tcgtaatatc atcaacgtcg gaatagagat cgaccgaact cggatcgtca cccatcagca
     5761 acgttttgat aattgcccgg cgcaaatgcg cggcaggttc tgtcccggcg atagtttcga
     5821 tagtaatcgt accgtcatcg aagttgatgt ggctgcgggt ctgatattga tcggtgtatt
     5881 tcacgtagtc cttaggaccg gcgatcacca cctctttgaa tccccagatg ttctcaatat
     5941 tgtgggcaaa ttgccccatc agaatatcaa aaccgttggt atctttgacc caggcttcgt
     6001 tataggtatc gccttttttg gtcgtcgaac aggagatgag caacggcgca atcaaagcca
     6061 gcgcgagata ttttttcatc attccgggtg cgtgttgtgt ttgtttttat gcaagttgcc
     6121 ggaggcgtgc tccggcactg tttttatttt ttatcttccg gcgtatagcc ctcgatatgc
     6181 acctctttac cctcgaacag gaagttgacc atctcctgct ccagcagctt gcggtgctcg
     6241 gcattcatca tgttgagttt cttttcatta atcagcatgg tttgtttgtg ctgccactgc
     6301 gcccaggctt ctttggagat ctcgttatag atgcgttttc ccagctcgcc ggggtacagc
     6361 tgaaaatcct gaccttctgc ttcacgttgc aggaaagtac aaaaaatcgt tctgctcata
     6421 aatcatcctc tttatcgact cacgcgctaa accggcgcgc cagtgcgtaa ctgctgtaac
     6481 aaacgctcca cgggagccgc caggccaact gacggcggtt gcgctaagtt ataccagagc
     6541 gcattgcctt catccatgca gccggtgaat gacgacacgg gaagccacat aggcacaata
     6601 tctaagtgga aatggctgaa ggtatgccga aacgcggtca gctgcgtcag gttatcggca
     6661 gcaatctgcc gttgcgccag ccactgccgc aaactttctt cgtcggcaaa ctgcgggaaa
     6721 cagtataaac cgccccacaa tccgctcggc ggacgctgcg ccagcaatac ttcatcttcg
     6781 tgctgtaata gcaaaaagta gccggtgcgc tccggcagcg tctgtttcgg ttttttgccc
     6841 ggataaagcg cccagctatt gttggcggcg gcaatacatc cgttttgtag cggacagagc
     6901 gaacatttcg gtttcgagcg cgtacaaatc atcgcaccca aatccatcat cgcctgatta
     6961 aaccgttcca cgccaaccgc gggcgtcacc tgctcgctca aactccataa tttattctcg
     7021 acctcttttt tccctggcca gccgcttaca gcatagcagc gcgccagcac ccgtttgacg
     7081 ttaccgtcga gaatcggaaa gtgcttaccc agagaaagcg agagaatcgc acctgcggtg
     7141 gaacgcccga cgcccggtaa cgccgcgact tcttcaaagg tttccgggaa tttaccgccg
     7201 tgtaaggtcg ccacttgttg tgccgcttta tgcaggttgc gcgcgcgggc gtaatagcca
     7261 agcccggtcc acaagtggag aacttcgtcg agcggcgcat tggcgagatc ggtcaccgtc
     7321 gggaagcgcg ccataaagcg ttcaaaatag gggataacgg tcgcaacctg agtttgttgc
     7381 aacatcactt ctgagagcca tactttgtag ggcgtcttgt caatttgcca gggcagcgtt
     7441 ttccgcccgt atttatcgta ccagtccaga acctgggctg aaaattgcga cgcttgcatg
     7501 gtcaccgaat tcactgttgt tgggggcaag attgcagcac agcgacggca gggtgtaaac
     7561 cggaactttc cgcagccacg gccttatcat gcttgcatcc ggcaactaac tttggataat
     7621 gcccgttttc agaacacttt cacaagcgac taaatcttta tgaaaaacga cgtcatttca
     7681 ccggaatttg atgaaaacgg ccgcccactg cgccgtatcc gtagttttgt gcgccgccag
     7741 gggcgactga ccaaaggcca ggaacatgcg ctggaaaact actggccggt gatgggcgtt
     7801 gagttcagcg aagatatgct ggatttcccc gcgctttttg gccgtgaagc gccggtgacg
     7861 cttgagattg gttttggcat gggggcgtcg ctggtggcaa tggctaaaga tcgccctgag
     7921 caggacttcc tcggcattga agtgcattca ccgggcgttg gtgcgtgcct ggcttctgcg
     7981 catgaagaag gtttaagcaa cctgcgcgtg atgtgtcacg atgcggttga agtgctgcat
     8041 aaaatgattc ctgacaattc attgcgcatg gtgcagctct ttttccctga cccgtggcac
     8101 aaagcgcgcc ataataaacg ccgtatcgta caggtgccgt ttgccgaact ggtaaaaagc
     8161 aaactgcagc tggggggcgt attccatatg gcgaccgact gggaacctta tgcggaacat
     8221 atgcttgaag tgatgtcttc tattgacggt tataaaaacc tgtcagagag caatgattac
     8281 gtaccgcgtc cggcatcacg tccggtgacg aaatttgaac aacgtggtca tcgtcttggt
     8341 cacggagtat gggacttaat gttcgagagg gtgaaataat ggcaaagaac cgtagccgtc
     8401 gtctgcgtaa aaaaatgcac atcgacgaat tccaggaatt aggattttcg gtggcatggc
     8461 gattcccgga aggtacatcg gaagaacaga ttgataaaac cgttgatgat tttattaacg
     8521 aggttatcga accgaacaaa ctggcctttg acggcagcgg ttatctggcc tgggaaggtc
     8581 tgatctgcat gcaggaaatc ggcaaatgca ccgaagaaca tcaggcgatt gtgcgtaagt
     8641 ggctggaaga gcgcaaactg gatgaggtac gcaccagcga acttttcgac gtttggtggg
     8701 actaagaaag catacgggcg atgacaaatg caaaactgcc tgatgcgcta cgcttatcag
     8761 gcctggaaag atgcacgatc gagtaggcgg gataaggtgt ttacgccgca tccggcatgg
     8821 aaaacgcgta ctttgttatc aatctggggc cagcaaatgc tggcctgatt ttttcttgag
     8881 ggaagactat gatgcgcaaa atgctgctgg cggcagcact ttcagtgacg gcaatgaccg
     8941 ctcacgccga ctaccagtgc agcgtcacgc cgcgtgacga tgtgattgtc agcccgcaaa
     9001 ccgtgcaggt gaagggcgaa aacggcaatc tggtgatcac gccagacggc aacgtgatgt
     9061 ataacggtaa gcaatattcc ctgaatgccg cccagcgcga gcaggcgaag gattatcagg
     9121 ctgaactacg cagcacgctg ccgtggattg atgaaggcgc gaaaagccgc gtcgaaaaag
     9181 cccgtattgc gctggataaa attatcgttc aggagatggg cgaaagcagc aaaatgcgca
     9241 gccgtctgac caaacttgat gcgcagctga aagagcagat gaaccgcatt atcgaaacgc
     9301 gcagcgatgg cctgacgttt cactataaag ccattgatca ggttcgcgcc gaaggccagc
     9361 aattagtgaa tcaggcaatg ggtggaattt tacaggacag cattaatgaa atgggcgcga
     9421 aagcggtgct gaaaagcggc ggtaacccat tacagaatgt gctgggaagc ctgggggggc
     9481 tgcaatcctc aatccaaacc gagtggaaaa agcaggaaaa agatttccag cagtttggca
     9541 aagatgtttg tagccgcgtt gtgactctgg aagatagccg caaagccctg gtcgggaatt
     9601 taaaataatc ctctatttta agacggcata atactttttt atgccgttta attcttcgtt
     9661 ttgttacctg cctctaactt tgtagatctc caaaatatat tcacgttgta aattgtttaa
     9721 cgtcaaattt cccatacaga gctaagggat aatgcgtagc gttcacgtaa ctggaggaat
     9781 gaaatggagt ttttcaaaaa gacggcactt gccgcactgg ttatgggttt tagtggtgca
     9841 gcattggcat tacccaatat caccatttta gcaaccggcg ggaccattgc cggtggtggt
     9901 gactccgcaa ccaaatctaa ctacacagcg ggtaaagttg gcgtagaaaa tctggttaat
     9961 gcggtgccgc aactaaaaga cattgcgaac gttaaaggcg agcaggtagt gaatatcggc
    10021 tcccaggaca tgaacgataa tgtctggctg acactggcga aaaaaattaa caccgactgc
    10081 gataaaaccg acggcttcgt cattacccac ggtaccgaca cgatggaaga aaccgcttac
    10141 ttcctcgacc tgacggtgaa atgcgacaaa ccggtggtga tggtcggcgc aatgcgcccg
    10201 tccacgtcca tgagcgcaga cggtccattc aacctgtata acgcggtagt ggccgcagct
    10261 gataaagcct ccgctaatcg tggcgtgctg gtggtgatga acgacaccgt actggacggt
    10321 cgcgatgtca ccaaaaccaa caccaccgac gtagcgacct tcaagtctgt taactacggt
    10381 cctctgggat acattcacaa cggtaagatt gactaccaac gtaccccggc acgtaagcac
    10441 accagcgata cgccattcga tgtctctaag ctgaatgagc tgccgaaagt cggcatcgtt
    10501 tataactacg ctaacgcatc cgatcttccg gctaaagcac tggtagatgc gggctatgat
    10561 ggcatcgtta gcgctggtgt gggtaatggt aacctgtata aatccgtgtt cgacaccctg
    10621 gcaaccgccg cgaaaaacgg cactgcagta gtgcgttctt cccgcgtacc gacgggtgct
    10681 accactcagg atgctgaagt ggatgatgcg aaatacggct tcgtcgcctc tggcacgctg
    10741 aacccgcaaa aagcgcgcgt cctgctgcag ctggctctga cgcaaaccaa agatccgcag
    10801 cagatccagc agatcttcaa tcagtactaa tcacctcgcc ccggtatcgt gccggggctt
    10861 tttcacttca gacttacgtc cattgccaat tttcattacc ctaatgataa tcaccggaat
    10921 aaattattcc gcgtgaggtt tttcgggtga aaaagcaatg gattgttggc acggcactgc
    10981 ttatgttgat gactggtaat gcctgggcag atggcgaacc gccaactgaa aatatcttaa
    11041 aagatcaatt caaaaagcag tatcacggca ttctcaagct tgatgctatc accttaaaaa
    11101 atcttgatgc taagggtaat caggccacct ggtcagcgga aggcgatgtc tcttccagtg
    11161 acgatctcta tacctgggtc ggtcagttgg cagattacga actgctcgaa cagacctgga
    11221 cgaaagataa accggtaaaa ttctcggcga tgttaaccag taaaggaacg cccgcgtctg
    11281 gctggtcggt gaacttttac tcttttcagg cggcagccag cgatcgtggg cgggtggtcg
    11341 acgatatcaa aacgaataat aaatatctga tcgtgaatag cgaagatttc aattatcgct
    11401 ttagtcagct tgaatctgcg ttgaataccc agaagaactc gattcctgcg ctggaaaagg
    11461 aagtgaaagc gcttgataag caaatggtgg cggcgcagaa agcagcggat gcatattggg
    11521 gaaaagatgc aaacggtaag caaatgaccc gcgaagaagc ctttaaaaaa attcaccaac
    11581 agcgtgatga gtttaataaa cagaacgata gcgaggcgtt cgcggttaaa tatgacaaag
    11641 aggtttatca accggcgatt gcggcatgtc ataaacagag tgaagagtgt tatgaagtgc
    11701 cgattcagca gaaacgagat ttcgatatca acgaacaacg gcgacaggct tttctgcaat
    11761 cacaaaaact cagccgtaaa ttgcaggatg actgggtaac gcttgaaaaa gggcaatatc
    11821 cgttaacgat gaaagtttca gaaataaaca gtaaaaaagt cgcgattttg atgaaaattg
    11881 acgatatcaa ccaagctaat gaacgctgga aaaaagatac cgaacaactg cggcgcaatg
    11941 gggtgattaa gtaagtttga tatagtgcct ttgccggata atataaaaag ttatccggca
    12001 aattattttt tacattagcc ctagtagctg tggaatgaac aaggatattt gcggaatgta
    12061 agtgatcatc atcaacgcta ctagcaatgc agcataaaat ggcagcaatg gtttgattaa
    12121 atgttgaatc ttcacacccg agatagaaca accaacaaat agcgcactac ccaccggcgg
    12181 tgttaacagc ccaatacaaa ggttggccac catcataata ccgaaatgta ctgggtccat
    12241 tcccagctct tgtgcaatcg gcaagaagat cggtgtaaag attagtacag ccggtgtcat
    12301 gtccataaag atgccgacaa ttaataagac gatattaatc agcagcaata tgatcagtgg
    12361 gttatcagaa atccccatca atgcgtcgct gatcatataa ggaatatcag cattagtcat
    12421 tgcccaggac atgcccacgg aaaaaccgat caacagtaat acaatcgaag tggtaacaac
    12481 cgactccaga atcagtttgg gtaaatggcg ccattttact tcccggtaaa ccagcaccga
    12541 gagaataaat gtgtagacaa cggcaattgc tgacgcttca gttgcggtaa aaatgccgcc
    12601 cagaatgccg cccatcacaa tgaacaccag aagcaaactt ggtaaggcat ccagaaacgc
    12661 tttacaggcc tgagcaaagg ttgctctttc tgaaagcggg tagccacggc gtttagcaat
    12721 aatgccgcag actatcatta ccgcaagacc cattaagatc cctggcaaat atcccgccat
    12781 aaatagcgat gctacagaaa cgccgccagc tgtcagggaa aacacaatta acacgtttga
    12841 gggcggtatc agtaatccag taatacaaga tgagacgttt acagcggtag aaaatgccgg
    12901 atcataacct tgtttggtct gaatgggatt cagtgtgcca ccaacggcag cagctgccgc
    12961 aacggctgaa ccagaaatgg aaccaaacat catattcgcc aggacgttca catgaccaag
    13021 tgaaccagga acccgaccaa ccattacctg ggcgaggtta atcaaacgta tcgcaatacc
    13081 gccgctgttc atcagcgttc cggcaaagat gaaaaagggg attgccaata gggcaaagtt
    13141 atccagtcca ttagccagtc gctgcgaaac agtgattgcc gcgatatccc agggaaacat
    13201 caaaagcatt gaagctacag tggcaatacc gattgagaac gaaatgggca caccaataaa
    13261 gaccaacaca aaaaaacagc caaacagtgt aatcgcgata taactatcca ttaactggcc
    13321 tcctgcgcat gttgtttcag ttgttgaatg ctttgattaa taaaacacaa ggcataaaac
    13381 atcatcacca atccacttaa tggcagaatg atatatacgt atcccatcgg tatttgcatc
    13441 gcggcagata cctgtgatgt tgccagcgtt ttatcaatta atttcaggcc accagtcaca
    13501 atgaccgaac cggcaaaacc taaaataaga acgttaatga ctatactaag aagtaattgc
    13561 ttgcgcttat tcagggcgag agcaaataaa tcgatagaga gatgtctctg cgcgccgacg
    13621 gtatatgcag cacctaataa ccctacccag atcatcaaaa atcgtgccag ctcatcggtt
    13681 aatgtacttg gctggttaag tacgtagcgg ctaaatactt gccagactac gcatacaacc
    13741 agagcaagca tgacggcgac agaaaacgcg gcaatgacgc ggtctaccgc aagtttcagg
    13801 cggttaaata tcattgtaag catcctggaa aatcagggcc agagactggc ccggaatatt
    13861 attgagcagc gttgtcgaac ttctctaaca aagcagcttg ttttggatct tttttgaagt
    13921 cgtcaaacag aggctgaact gcggcacgga atggtgcttt atccactttt acaatttcac
    13981 cccccatagc tttcgcctga gcacgtgtgt cagcgtcaat tttttcccac aatttttgtt
    14041 gataagcttc ggatttcttc gctgctgttt cgaggatttg ttgctgttct ggggttaatt
    14101 tattccatgt tttggtggag ataaccaaaa agtcaggaat tgaggcgtgt tcatcttcag
    14161 aaaaaacttt ggcaatttca atatgtcgag tctgcaccca ggaaggcacg ttattttctg
    14221 cgccatctac tacaccttgc tgcatcgcag tataaacttc gccaaatgaa attggagtgg
    14281 gtgaaccacc catcagttca atcattttga tggtggtcgg acttggctga acacgaattt
    14341 tcagcccttt aagatcttct ggcttagtaa tcggtttttt cgcatagaaa ctgcgtgtac
    14401 ccgctacata agctgataaa gcaaaaaagc ctttgtcttt tgttgagtcc ataatttctt
    14461 taccaacttc accaaaaaca actttgttga agtgagcctg atctttaaat aagaacggca
    14521 aattatagat agcataaata ttatcaaaag actccagatc actggcggaa cctttggtca
    14581 tatccagagc gccattttgc aacaactcca tagtttctcg cgcattaccc atctggctgc
    14641 tgggataaat acggatcacc atattacctt ttgacagctg ttttacctct ttcgccagct
    14701 cttcaaaact ctgatgtaca acatgacttc tttccaggtt gtgtgctaac tttaacgtca
    14761 ctttttctgc ggcgtttacg ccagtgctaa ccagagccag caaggatgcg cagactaatg
    14821 agcgggtaaa tgacctcatt ttcataaaaa aactccctgt tgaaggatgg aaattggttg
    14881 atatctaatt gtgcttatca gtgctttgtg cgacaaattg gttaacctgt ctgacaactt
    14941 gtctatgttt atcacaactt gaaataaagt cagaaattaa atattaaatt tgtgcgccag
    15001 gttgtacatg tttataatca ataa
//
LOCUS       DAZGJW010000116        14746 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZGJW010000116 DAZGJW010000000
VERSION     DAZGJW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.116"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..296
                     /locus_tag="ACHKIK_003608"
     CDS             <1..296
                     /locus_tag="ACHKIK_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HFO9084706.1"
                     /translation="WWRALLQSRNDHLRQTALRNAHTPASLLTTLTESRHRSLAMNNP
                     QLAADVKTTWLKEDPSLLLFVEQPDLSLLRDLVKTGAMRKIRSEARHWLEEKQ"
     gene            293..376
                     /locus_tag="ACHKIK_003609"
     CDS             293..376
                     /locus_tag="ACHKIK_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084707.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(454..1365)
                     /gene="rdgC"
                     /locus_tag="ACHKIK_003610"
     CDS             complement(454..1365)
                     /gene="rdgC"
                     /locus_tag="ACHKIK_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO9084708.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            1490..2398
                     /gene="mak"
                     /locus_tag="ACHKIK_003611"
     CDS             1490..2398
                     /gene="mak"
                     /locus_tag="ACHKIK_003611"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO9084709.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(2541..3725)
                     /gene="araJ"
                     /locus_tag="ACHKIK_003612"
     CDS             complement(2541..3725)
                     /gene="araJ"
                     /locus_tag="ACHKIK_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO9084710.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCCGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(3851..6994)
                     /gene="sbcC"
                     /locus_tag="ACHKIK_003613"
     CDS             complement(3851..6994)
                     /gene="sbcC"
                     /locus_tag="ACHKIK_003613"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO9084711.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRQDELQQEASRRQQALQQVLAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQRIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASETQEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(6991..8193)
                     /gene="sbcD"
                     /locus_tag="ACHKIK_003614"
     CDS             complement(6991..8193)
                     /gene="sbcD"
                     /locus_tag="ACHKIK_003614"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO9084712.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            8383..9072
                     /gene="phoB"
                     /locus_tag="ACHKIK_003615"
     CDS             8383..9072
                     /gene="phoB"
                     /locus_tag="ACHKIK_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO9084713.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            9130..10425
                     /gene="phoR"
                     /locus_tag="ACHKIK_003616"
     CDS             9130..10425
                     /gene="phoR"
                     /locus_tag="ACHKIK_003616"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO9084714.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWLRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            10832..12151
                     /gene="brnQ"
                     /locus_tag="ACHKIK_003617"
     CDS             10832..12151
                     /gene="brnQ"
                     /locus_tag="ACHKIK_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO9084715.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            12227..13600
                     /gene="proY"
                     /locus_tag="ACHKIK_003618"
     CDS             12227..13600
                     /gene="proY"
                     /locus_tag="ACHKIK_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO9084716.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            13756..>14746
                     /gene="malZ"
                     /locus_tag="ACHKIK_003619"
     CDS             13756..>14746
                     /gene="malZ"
                     /locus_tag="ACHKIK_003619"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO9084717.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFDGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWS"
BASE COUNT         3231 a         3780 c         3993 g         3742 t
ORIGIN      
        1 aatggtggcg tgcattgctg caaagtcgca atgatcattt gcgccaaacg gcattacgca
       61 acgcacatac cccggcgtcg ttgttaacga cattgaccga gtctcggcat cggtcgctag
      121 ctatgaataa tccacagctc gctgccgatg tgaaaacgac gtggttaaaa gaggatccat
      181 cattactctt atttgtcgaa caacccgatc tttcgctgtt acgggattta gtgaaaaccg
      241 gggcaatgcg gaaaattcgc agtgaagcgc gtcactggct tgaggaaaaa caatgacaca
      301 acgtccatgg agcaagttac agcataaaac gcataatatt gctgcgctaa aaattattgc
      361 ccgccgtagc gaataattat atgcctggtg tggcttcgta cgccggataa gacgcggcag
      421 gcgtcgcatc cggcattaaa ggaaaatcag caattaacgt tgtgcttcgc cacctaatcc
      481 ttcaatcagg ttttgaatta acgctgccag ttcaccagtc atcaggatga aatcggcatc
      541 aaaacgctgg gcgaaatctt cacggtcgat atcttcgttt tgatcgcgca gctcgtcgca
      601 gaacttcaga cgcttgagcg aaccatcgtc gcacatcaca aactgaatgc gctgctgcca
      661 gtcgagcgcc agtttagtca ccacttttcc ggcttcaatg tgattggtga tctcttcgct
      721 ggtcagatct tgtttcttcg cgcggatcac gccgccatct tccagcaacg atttcagctc
      781 ggcttcatca agcagctgga agccctgtgc cgcactaccg gagcgaaccc attcggtcag
      841 cgtcagttca atcgggtttt ccatgctcaa tggtacaacc ggtaacgacc ccaggctttt
      901 acgcagtaat gccagcgtat cttccgcttt tttggcactg gcgcagtcca ccataatcaa
      961 accgttaacc gtgtcgatcc acatcattgt ctggctaaaa cggctgaaag cacgcggcag
     1021 cagagagtgc agcacttcgt ctttcagcga atctttttcg gttttcttca gcttacgcgc
     1081 ctgttccgct tccagtttgg cgattttcgc ttccagcgcc tgtttaatca ccggagacgg
     1141 gaggattttt tcttctttgc gcgcgcagat aacaatttga ccattggcaa cgtgcgttaa
     1201 cgcatcgctg tgcgatccca tcggaggaac ccagcccatc ttcgccatgt cctggctgcc
     1261 gcatggggta aatgccatcg aggctagctg tttttccatc tcttctgcac gcagcgaaat
     1321 ctcgcggcta agacggtaaa ccattaaatt tttgaaccac agcatgataa tttccacggc
     1381 cttgtcgtta aatttagcgg gcatgataac gaattgtcgg cggccttgca ttgccaatcc
     1441 ggttgtccgt ctctacgcta ttgatattga aaaaaataag gagagtaccg tgcgtatagg
     1501 tatcgattta ggcggcacca aaactgaagt gattgcactg ggcgatgcag gggagcagtt
     1561 gtaccgccat cgtctgccca cgccgcgtga tgattaccgg caaactattg aaacgatcgc
     1621 cacgttggtt gatatggcgg agcaggcgac agggcagcgc ggaacggtag gtatgggcat
     1681 tccaggttcg atttcgccct acaccggtgt ggtgaagaat gccaattcaa cctggctcaa
     1741 cggtcagcca ttcgataaag acttaagcgc gcggttgcag cgggaagtgc ggctggcaaa
     1801 tgacgccaac tgtctggcgg tttcagaagc ggtagatggc gcggcagcgg gagcgcagac
     1861 ggtatttgcc gtgattatcg gcacgggatg cggcgcgggc gtggcattca atgggcgggc
     1921 gcatatcggc ggcaatggca cggcaggtga gtggggacac aatccgctac cgtggatgga
     1981 cgaagacgaa ctgcgttatc gcgaggaagt cccttgttat tgcggtaaac aaggttgtat
     2041 tgaaaccttt atttcgggca ctggattcgc gacggattat cgtcgtttga gcggacatgc
     2101 gctgaaaggc agtgaaatta tcagcctggt tgaagaaagc gatccggtag cggaactggc
     2161 attgcgtcgc tacgagctgc ggctggcaaa atcgctggca catgtcgtga atattctcga
     2221 tccggatgtg attgtcctgg ggggcgggat gagcaatgta gaccgtttat atcaaacggt
     2281 tccgcagttg attaaacaat ttgtcttcgg cggcgaatgt gaaacgccgg tgcgtaaggc
     2341 gaagcacggt gattccagcg gcgtacgcgg cgctgcgtgg ttatggccac aagagtaaaa
     2401 aacgtaggca attggcgcat catgcctgat gcgacgcttg ccgcgtctta tcaggcctac
     2461 aaaatgtgcc agaaccgcgt agggcggata aggcgttcac gccgcatccg gcaataagca
     2521 acgcgcaccg tgacagtaat ctaccccagt ggtttcgcca gcaccggact atccgccgct
     2581 tgctggcgct tatagcgacc atacagcagc aacgatgaca tcgcagcaaa cgaaagcagg
     2641 gcggcaggca gcgccacgta attatatgcc agccccagcg tcagcatcat acctccgcaa
     2701 tatgcgccga cggcgctacc gaggttaaac gctatttgcc cacctgcggc acctaataac
     2761 tctccgcctt tggcgttttg tagtaacaat atttgcagag gtgctgaaag ggcaaataat
     2821 cccgcgcaac aaataaaagc aaaaataagc gacgttgttt tcatgccgca gcagaaaaag
     2881 agcatcagca gtgccagtac aattataaag tcagtcactg ctgcaatgcg cagtggtgaa
     2941 taacgtcctg aaatcctgcc acttagcata tttcccaaca ccatccctag cccaactaac
     3001 atcataataa aggtcatcgc cgtttccgaa aaaccggaaa taaacatcat gtatggcttt
     3061 acgtagctga accaggcaaa cacacctgcg ttgccaaaca tcgtggcggc gaaaattaac
     3121 cacggggccg ggctgcgtaa aaagtgaaat tgttcgcgca gctttccttt cgcctcgtcg
     3181 cgaatatccg gcacccaaaa atagaccgat gccatcaccg caatattaaa aacagcgatc
     3241 aataaaaagg tgtaacgcca gctaaattcc tgacttaaat acgttcccag cggaatgccc
     3301 agcaaattgg cgactgtcat cccggaaacc atccccgcca cggcggcggt gacttttccg
     3361 ggtttgataa tttttgataa cacgatcgcg ccgacgccaa aaaatgcgcc atgcggaaag
     3421 ccggaaacca ggcgaccaat ggcaagcatc aggtaagacg aagagagcgt gaacatggcg
     3481 ttgccaatga cgcacaacgc caccagaaac aacaagatat gtttgagtga gtagcggctg
     3541 gaaaagagtg cgatgattgg cgcaccgacc accactccca gcgcataata cgagatcata
     3601 tgcccggcag caggaatcga aattcctacg ttatgcgcca gctccgtgag cacgcccata
     3661 atgccaaatt cggccatccc caaaccaaac gtgcccagag ccaaagataa aatgactttt
     3721 ttcataccac catcacaact accagaacgg ctaccaatca ccgtagccaa taaagtgata
     3781 gcttgcagga taacgaccag caaggccaag agataattcg cccctctgta ttcattatcc
     3841 tgctgaatag ttatttcact gcaaacgtac tttccagttt gctgtagcct aggccgttga
     3901 tctttttcac tttgatctgc accggaatac gctctttcat cgcttctacg tggctaatca
     3961 caccgatggt tttgccactg gcgttcaggg catccagcgc atcaagggcg gtatccagcg
     4021 tttcgctatc cagcgtgcca aaaccttcat caaggaacag cgagtcaata cgtgttttat
     4081 ggctgaccag atccgaaagc gccagcgcca gcgccagact aacgaggaaa ctttcgccgc
     4141 cggaaagggt acgggtatcg cgtaccgcat ctgcctgcca ggtatcaaca acctcgactt
     4201 cctgcgtctc gctggctttg cgctgtaaca gatagcgccc gtgcagccgg gtaagttgct
     4261 gattagcgag atggactaaa ttatccagcg tcagcccctg ggcaaacttg cggaatttat
     4321 cgccctcttt ggaacctatt agcgaattca gatatcccca gtcctcaacc tgctgcgtca
     4381 tttgagcaat ttgctgcatt aaggtttgtt gttgctgacg gttatctgca tcctgcttca
     4441 gctgctggcg aatctcgcct tgactcgtcg tgttttcacg caacttttgg tgagtttgcg
     4501 ctaactcttg ctgaatctgc tccaccgtca cagtgagagc caacccgtca ggtcggtgtt
     4561 gttgatgctg tgccagcgtt tctgctgtct gagtgaccag cgtttgcgcc tgacggcgct
     4621 ggttttccag attctgcttg agctgttcca gctgcgttag tgtttgttca tccattagcg
     4681 ccgcaaggaa cgcctgctga tcgtcaaaga cgctggcctg tagcgcggtg tcaaactgcg
     4741 cctgggcttt ttgcagactt tgcgccgcca gaacatcctg ttgctgtaat gtctgctgct
     4801 ggctgtgtaa tgcgagacat tgttcatgaa cctgccgcca gttatccagc gccacagttt
     4861 cttcgctgtg cgggagatca tcactttgcg gcaacgtttc cagaatcggc gtcagctgct
     4921 gaatacggtt ttgcagcgcg gttaattcgt tctggcgttg ctgccagctc tgcgcttctt
     4981 gctgacgtgt cgccaaccag ctctcttctt catcttcctg tggcaatgtc agtgcataac
     5041 ccgccaatgc cgttaagagc tgctgctggc gttgttcaat ttgctgttga tactggataa
     5101 tttgctgatt atgcgcggca atctgccctt gtaattcatg tcgttggctg agtaaccgca
     5161 gctggcgttc gtgctcatct tgtgcatcaa gccacggttg aatatcgtcc tgtggctgca
     5221 aggtgatatt gaggctggcc gtgacggctt gccattgttg agtaagtgct tgctcatctt
     5281 gtcggaggct ttgcgcttcg ttttcatcgc gctgaagctg cttcgttaat gcatccagtt
     5341 gcccgcgcag cgccgcacct tcttcgccga gctttttaac ttcgttttcc agcgccagta
     5401 atcgagactg attaacgcca ggctccagcg cctgatacgc ctcgaccgcc ggatggctgg
     5461 tggaaccaca aagtggacaa ggctgacccg cctgtaactg tgcacgctgg gcttccagcg
     5521 ttttgatgcg cgcttcctgc tcgcaaatgg ttttcacatc ggcaagttgc tgcgtctttt
     5581 ctttataacg ctggcgcatt tcgttaagtg cgacattacg ttgcgtctgt tccagagtga
     5641 cattctggat agtgaccatt aactgcgcca gacgtttttg ttggggaaca atctgcccat
     5701 gcagcgcgac caggcgctga cgcagtgggc gttgctcagc atgttgcgcc agggcggtag
     5761 caacttcatc ggcggttaac gtcaacgtga tcgccgcaag cgcattaagt ttttgctcag
     5821 catgggttaa ctgttgctgc cattgccgca gatgctcgcg atcgctggtt tgttgggaga
     5881 actgcgcacg ccaacccgcc agttcgttgt tccactgacg gaagcggtcg tgttcctgta
     5941 accaggtatt caggctttgt tgctgctgct gtaattctgc tgactgcttc gccgcgtggt
     6001 ggcgaatgct cgcgcgaagc gccattgtgc tctgtaagcg agtatttact tcttcaatcc
     6061 gctggcgaat atgcgccagc gccgcgctgt gttctgcgat gcgttcccag tgtggacgaa
     6121 gatttcgtgc cggttgtgcc agactaagcg ccgccagttg aggttgcgct ttttcttctt
     6181 cggctaacac ctgttgcaag gcctgctgac ggcggctggc ttcttgctgc aattcgtcct
     6241 gacgcgttaa ccagtttagc gattgttgtt cttgctgctg cgcggtaagt aactgttttt
     6301 cttcgtcagt aagtacctgc aaactcgctg tcagcgattg cacttgttcc ggcgtgagca
     6361 acgcgacgcc gctggcctgc gcttgcagct tctccagctc tgtgcgggcc gatttgtgct
     6421 gctcaaaaac catcgccgag atttgcccgt agatttcagt gccggttaac tcctcgagca
     6481 attccgcgcg ttctttgggt ttggcattca ggaaggcagc aaattgcccc tgcgaaagca
     6541 gcatcgaacg ggtgaagcgc ccgtaatcca gcccggttaa cgtcgctgtc agttccagct
     6601 tatctttcac tttgtcggcg agaattttgc cgtcggcgca gcgcgccagc tctacgcgtg
     6661 gcacctgcaa attaccgtcg ggttggttac gcgcccgatt ctggctccag aatgcacggt
     6721 acgcttcacc tttcacttca aactccacct ccgccagaca ttcggcggta tcgcgggtca
     6781 tgagatcatt ttgcgattgt gaaacgttag agagacgcgg agtttcatga tacagcgcca
     6841 gacaaatggc gtccagcagg gtggttttcc ccgcacctgt tgggccggta atagcaaaca
     6901 gcccgttgct ggcgaacggc tcgcgggtga aatcaatctt ccattcgcct tttaatgagt
     6961 tcaggttttt caggcgcagg ctgagaattt tcatgcttcg tgttctccgg cgagggtatg
     7021 caacgtcgtg gtgaaaagat gctgcagacg ttgctgctgc gattcatcca gttcttccag
     7081 tgccagacgg cgattgaaca cctcttcgac gctgagttcg ctgagggttt cacgctgttg
     7141 gctggctaac acacgctcgc gctgttcacg actccgacgt accagcaata cttcgacagg
     7201 caatgattcg gttaatgcct ggattttgcg ctgaatatca tgcagatact catcagtagt
     7261 gatttcgata tccagccaga caggtggctc ctgcgataca tcgcgccact gttccagctg
     7321 tgcggtaatc gacgccagat cgcctttcag cactgccatg ggttgcgtta ccggtacgtt
     7381 caggttttcc acgctctcta atttgccgtt tgaaaatgtc accagatgga catatttact
     7441 cttaccgcat tcatcaaaac tcagtggaat gggggagccg caatagcgaa catgttccat
     7501 gccgccaata atctgtgcgc ggtgaatatg cccgagcgcg atgtagtcgg ctggtggaaa
     7561 gttttgtgcc ggaaacgcgt ccagcgtgcc aatataaatg tcacgcacgg cgtcactttt
     7621 actggccccc acggtcgtta aatgtcccgt ggcgatgatg ggcagaggct gatcgccgcg
     7681 cagtttgcag gcatcggcat agtgttgttg gtaataatcg gtaatcgctg ccagtaaatg
     7741 ctgctgtttt tcaataccgt taagccccgc ctggctggta ataatgtcac gtggacgtaa
     7801 aaacggaatg gggcacagca ctgcgcctgg cgtcccgtcg cgacgaggca agatttgcgg
     7861 cgcatgtccg gcgctggcga ccacggtagt attgaggaac gccatgatat cgcgcgattc
     7921 attcagcgtg gcgaccgagt catggtttcc tgccagtacc accagatgac agccagtttg
     7981 ctgtaaattg acaacaaaac ggttgtataa cgtgcgggcg taactgggcg gcgagccggt
     8041 atcgaaaaca tcaccggcaa caataatcgc atccacctga tgggtttgtg ctgtctccag
     8101 cagccagtca agaaaagcct gatgttcagc ttcgcggctt ttactgtaga agttctggcc
     8161 gagatgccag tctgaggtgt gaaggatgcg cataacggtt ccctggcgaa aaagcatggg
     8221 cgcgattata cccaaacaga tgtgccattt gcttttttct gcgccacgga aatcaataac
     8281 ctgaagatat gtgcgacgag cttttcataa atctgtcata aatctgacgc ataatgacgt
     8341 cgcattaatg atcgcaacct atttattaca acagggcaaa tcatggcgag acgtattctg
     8401 gtcgtagaag atgaagctcc aattcgcgaa atggtctgct tcgtgctcga acaaaatggc
     8461 tttcagccgg tcgaagcgga agattatgac agtgctgtga atcaactgaa tgaaccctgg
     8521 ccggatttaa ttctccttga ctggatgtta cctggcggct ccggtatcca gttcatcaaa
     8581 cacctcaagc gcgagtcgat gacccgggat attccagtgg tgatgttgac cgccagaggg
     8641 gaagaagaag atcgcgtgcg cggccttgaa accggcgcgg atgactatat caccaagccg
     8701 ttttcgccga aggagctggt ggcgcgaatc aaagcggtaa tgcgccgtat ttcgccaatg
     8761 gcggtggaag aggtgattga gatgcaggga ttaagtctcg acccgacatc tcaccgagtg
     8821 atggcgggcg aagagccgct ggagatgggg ccgacagaat ttaaactgct gcactttttt
     8881 atgacgcatc ctgagcgcgt gtacagccgc gagcagctgt taaaccacgt ctggggaact
     8941 aacgtgtatg tggaagaccg cacggtcgat gtccacattc gtcgcctgcg taaagcactg
     9001 gagcccggcg ggcatgaccg catggtgcag accgtgcgcg gtacaggata tcgtttttca
     9061 acccgctttt aacgccttgc tcatcggacg cagagcaggg cttatgattt cttaactgga
     9121 gtatcttacg tgctggaacg gctgtcgtgg aaaaggctgg tgctggagct gctactttgc
     9181 tgcctcccgg ctttcatcct gggtgcattt tttggttacc tgccctggtt tttgctggca
     9241 tcggtaacgg gactgcttat ctggcatttc tggaatttat tgcgcctttc atggtggctg
     9301 tgggtggatc gtagtatgac cccgccaccg gggcgtggta gctgggaacc gctactatac
     9361 ggcttacacc agatgcagct gcgaaataaa aaacgccgcc gtgaactggg caatctgatt
     9421 aaacgctttc gtagcggcgc ggagtcgctg cccgacgcag tggtgctgac cacggaagag
     9481 ggcggtattt tctggtgtaa cggtctggcg caacaaattc ttggtttgcg ctggccggaa
     9541 gataacgggc agaacatcct taacctgctg cgttacccgg agtttacgca atatctgaaa
     9601 acgcgtgatt tttctcgccc gctcaatctg gtgctcaaca ccgggcggca tctggaaatt
     9661 cgcgtcatgc cttataccca caaacagttg ctgatggtgg cgcgtgatgt cacgcaaatg
     9721 catcaactgg aaggggcgcg gcgtaacttt tttgccaacg tgagccatga gttacgtacg
     9781 ccattgaccg tgttacaggg ttacctggag atgatggatg agcagccgct ggaaggcgcg
     9841 gtacgcgaaa aagcgttgca caccatgcgc gagcagaccc agcggatgga agggctggtg
     9901 aagcagttgc taacgctgtc gaaaattgaa gccgcgccga cgcaattgct caatgaaaag
     9961 gttgatgtgc cgatgatgct gcgcgttgtt gagcgcgagg ctcagactct gagtcagaaa
    10021 aaacagacat ttacctttga gatagataac ggcctcaagg tgtctggcaa cgaagatcag
    10081 ctacgcagtg cgatttcgaa cctggtctat aacgccgtga atcatacgcc ggaaggcacg
    10141 catatcaccg tacgctggct gcgagtgccg cacggtgccg aatttagcgt tgaagataac
    10201 ggaccgggca ttgcaccgga gcatattccg cgcctgaccg agcgttttta tcgcgttgat
    10261 aaagcgcgtt cccggcaaac cggcggtagc ggattagggt tagcgatcgt gaaacatgcg
    10321 gtgaatcatc acgaaagtcg cctgaatatt gagagtacag taggaaaagg aacacgtttc
    10381 agttttgtta tcccggaacg tttaattgcc aaaaacagcg attaatccgc ctttgtcatc
    10441 ttttattgcc ataagccagc cgatgctggc ttattttctt tgcagtcaaa atacgggcgt
    10501 tagattttac aacggttggt gattttttgt tcgcatgatt agccatgtct ttttcacgga
    10561 aatagtgttt tatactggtt ggtgatttct tatcgctata tacctctggt ttttagatcc
    10621 ctccttgctt taaaacgtta taagcgttta aattgcgctt caggtgctgt catactgact
    10681 gcattaacgc ggtaaatcga aaaactattc ttcgccgcgc ctgattggga gtatttcccg
    10741 ctaaaattgt ttaaatatac cgctgtatca tccccaggga ttggcacaaa aatttaacgt
    10801 tacaacacca catccacagg cagtatgatt tatgacccat caattaagat cgcgcgatat
    10861 catcgctctg ggctttatga catttgcgtt gttcgtcggc gcaggtaaca ttattttccc
    10921 tccaatggtc ggcttacagg caggcgaaca cgtctggact gcggcattcg gcttcctcat
    10981 tactgccgtt ggcctgccgg tattaacggt agtggcgctg gcaaaagttg gcggcggtgt
    11041 tgacagcctc agcacgccaa tcggtaaagt cgctggcgta ctgctggcaa cggtttgtta
    11101 cctggcggtg gggccgcttt tcgctacgcc gcgtacagct accgtttcct ttgaagtggg
    11161 gattgcgccg ctgacgggtg attccgcgct gccgctgttt atctacagcc tggtctattt
    11221 cgctatcgtt attctggttt cgctctatcc gggcaagctg ctggataccg tgggcaactt
    11281 ccttgcgccg ctgaaaatta tcgcgctggt catcctgtct gttgccgcga ttgtctggcc
    11341 ggcgggttct atcagcacgg cgactgaggc ttatcaaaac gctgcgtttt ctaacggctt
    11401 cgttaacggc tatctgacca tggatacgct gggcgcaatg gtgtttggta tcgttattgt
    11461 taacgcggcg cgttctcgtg gcgttaccga agcgcgtctg ctgacccgtt ataccgtctg
    11521 ggctggcctg atggcgggtg ttggtctgac tctgctgtac ctggcgctgt tccgtctggg
    11581 ttcagacagc gcgtcgctgg tcgatcagtc tgcaaacggc gctgctattc tgcatgctta
    11641 cgttcagcac acctttggcg gcggcggtag cttcctgctg gcggcgttaa tcttcatcgc
    11701 ctgcctggta acggcagttg gcctgacctg tgcttgtgca gaattctttg cccagtacgt
    11761 accgctctct tatcgtacgc tggtgtttat cctcggcggc ttctcgatgg tggtttctaa
    11821 cctcggctta agccagctga tccagatctc cgtaccggtg ctgaccgcta tttatccgcc
    11881 gtgtatcgca ctggttgtat taagttttac acgctcatgg tggcataatt cgtcccgcgt
    11941 gattgctccg ccgatgttta tcagcctgct ttttggtatt ctcgacggga tcaaagcatc
    12001 tgcattcagc gatatcttac cgtcctgggc gcagcgttta ccgctggccg aacaaggtct
    12061 ggcgtggtta atgccaacag tggtgatggt ggttctggcc attatctggg atcgcgcggc
    12121 aggtcgtcag gtgacctcca gcgctcacta aatcactgaa catttgtttt aaccacgggg
    12181 ctgcgatgcc ccgtggtttt ttattgtgtt gatgggttag gaattgatgg aaagtaagaa
    12241 caagctaaag cgtgggctaa gtacccgcca catacgcttt atggcactgg gttcagcaat
    12301 tggcaccggg ctgttttacg gttcggcaga cgccatcaaa atggccggtc cgagcgtgtt
    12361 gttggcctat attatcggtg gtatcgcggc gtatatcatt atgcgtgcgc tgggggaaat
    12421 gtcggtacat aacccggcag ccagctcttt ctcgcgttat gcgcaggaaa acctcggccc
    12481 gctggcaggt tacattaccg gctggaccta ttgctttgaa atccttattg tcgccatcgc
    12541 cgatgtgacc gcttttggta tctatatggg tgtctggttc ccgacggtgc cgcactggat
    12601 ttgggtactg agcgtggtgc tgatcatttg cgccgtaaac ctgatgagcg tgaaggtctt
    12661 cggtgagctg gaattctggt tctcgttctt taaagtcgcc accatcatca tcatgattgt
    12721 cgccggtttc ggcatcatca tctgggggat tggcaacggc gggcaaccga ccggtattca
    12781 taacctgtgg agcaacggcg gcttcttcag taacggctgg cttgggatgg tgatgtcgtt
    12841 gcaaatggtg atgtttgctt acggtgggat cgaaattatc gggattaccg ccggtgaagc
    12901 gaaagatcct gagaaatcga taccgcgtgc gattaactcc gtgccgatgc gtattctggt
    12961 gttctacgtc ggtacgctgt tcgtcattat gtctatctac ccgtggaatc aggttggcac
    13021 tgccggtagc ccgttcgtgc tgacgttcca gcatatgggc attacctttg ccgccagcat
    13081 tcttaacttt gttgtgctga ctgcttcgct gtcggcaatt aacagtgacg tatttggcgt
    13141 aggccgtatg ctccacggta tggcagagca gggcagcgcg ccgaaaattt tcagcaaaac
    13201 gtcgcgtcgc ggtattccgt gggttacggt gctggtgatg actaccgcgc tgctgtttgc
    13261 ggtgtatctg aactacatca tgccggaaaa cgtcttcctg gtgatcgctt cgctggcaac
    13321 cttcgccacg gtgtgggtgt ggattatgat cctgctgtcg caaattgcct tccgtcgccg
    13381 tttgccgcca gaagaagtta aggcgctgaa atttaaagtg ccgggtgggg tagcaacgac
    13441 catcggcggg ctgattttcc tgctctttat tatcgggttg attggttatc acccggatac
    13501 gcgtatctcg ctgtatgtcg gtttcgcgtg gattgttgtg ctgttgattg gctggatgtt
    13561 taaacgccgc cacgatcgtc agctggctga aaaccaataa tacttgcgtt ctgtaagccg
    13621 gttcaggagg aaatcctgat ccggctattc cgaaagttat ccgcccccgt cctcctcccc
    13681 caaatatcct tcagatgatg agtgatcctg cattaggcta tggcaaggtg atcagatttt
    13741 catcacaggg gaattatgat gttaaatgca tggcacctgc cggtgccccc atttgttaaa
    13801 caaagcaaag atcaactgct cattacactg tggctgacgg gcgaagaccc accgcagcgc
    13861 attatgctgc gtacagaaca cgataacgaa gaaatgtcag tatcgatgca taagcagcgc
    13921 agtcagcagc agcctggcgt caccgcatgg cgtgcggcga ttgatctctc cagcggacaa
    13981 ccccggcggc gttacagttt caaactgctg tggcacgatc gccagcgttg gtttacaccg
    14041 cagggcttca gccgaatgcc gccggcacga ctggagcagt ttgccgtcga tgtaccggat
    14101 atcggcccac aatgggctgc ggatcagatt ttttatcaga tcttccctga tcgttttgcg
    14161 cgtagtcttc ctcgtgaagc tgaacaggat catgtctatt accatcatgc agccggacaa
    14221 gagatcatct tgcgtgactg ggatgaaccg gtcacggcgc aggcgggcgg atcaacgttc
    14281 tatggcggcg atctggacgg gataagcgaa aaactgccgt atctgaaaaa gcttggcgtg
    14341 acggcgctgt atctcaatcc ggtgtttaaa gctcccagcg tacataaata cgataccgag
    14401 gattatcgcc atgtcgatcc gcagtttgac ggtgatgggg cgttgctgcg cttacgccac
    14461 aatacgcagc agctgggaat gcggctggtg ctggatggcg tgtttaacca cagtggcgat
    14521 tcccatgcct ggtttgacag gcacaatcgt ggcacggggg gagcttgtca caaccctgaa
    14581 tcgccctggc gcgactggta ctcgtttagt gatgatggca cggcgctcga ctggcttggc
    14641 tatgccagct tgccgaagct ggattatcag tcggaaagtc tggtgaatga aatttatcgc
    14701 ggggaagaca gtattgtccg ccattggctg aaagcgccgt ggagta
//
LOCUS       DAZGJW010000117        14599 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZGJW010000117 DAZGJW010000000
VERSION     DAZGJW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.117"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            54..377
                     /locus_tag="ACHKIK_003620"
     CDS             54..377
                     /locus_tag="ACHKIK_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO9084718.1"
                     /translation="MKWIVIDTIIQPSCGISFSAIWGNMKMIIWYQSTIFLPPGSIFT
                     PVKSGIILKDKEYPITIYNIAPFNKDLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(480..1730)
                     /gene="icd"
                     /locus_tag="ACHKIK_003621"
     CDS             complement(480..1730)
                     /gene="icd"
                     /locus_tag="ACHKIK_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO9084719.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1902..2555
                     /gene="rluE"
                     /locus_tag="ACHKIK_003622"
     CDS             1902..2555
                     /gene="rluE"
                     /locus_tag="ACHKIK_003622"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO9084720.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2565..3026
                     /gene="nudJ"
                     /locus_tag="ACHKIK_003623"
     CDS             2565..3026
                     /gene="nudJ"
                     /locus_tag="ACHKIK_003623"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO9084721.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            3080..4186
                     /gene="mnmA"
                     /locus_tag="ACHKIK_003624"
                     /gene_synonym="trmU"
     CDS             3080..4186
                     /gene="mnmA"
                     /locus_tag="ACHKIK_003624"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO9084722.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4222..4863
                     /gene="hflD"
                     /locus_tag="ACHKIK_003625"
     CDS             4222..4863
                     /gene="hflD"
                     /locus_tag="ACHKIK_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO9084723.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4867..6237
                     /gene="purB"
                     /locus_tag="ACHKIK_003626"
     CDS             4867..6237
                     /gene="purB"
                     /locus_tag="ACHKIK_003626"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO9084724.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6405..7076
                     /gene="phoP"
                     /locus_tag="ACHKIK_003627"
     CDS             6405..7076
                     /gene="phoP"
                     /locus_tag="ACHKIK_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO9084725.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            7076..8536
                     /gene="phoQ"
                     /locus_tag="ACHKIK_003628"
     CDS             7076..8536
                     /gene="phoQ"
                     /locus_tag="ACHKIK_003628"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO9084726.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8612..9733
                     /gene="roxA"
                     /locus_tag="ACHKIK_003629"
                     /gene_synonym="ycfD"
     CDS             8612..9733
                     /gene="roxA"
                     /locus_tag="ACHKIK_003629"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO9084727.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9879..11108)
                     /gene="pepT"
                     /locus_tag="ACHKIK_003630"
     CDS             complement(9879..11108)
                     /gene="pepT"
                     /locus_tag="ACHKIK_003630"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO9084728.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            11358..12494
                     /gene="potA"
                     /locus_tag="ACHKIK_003631"
     CDS             11358..12494
                     /gene="potA"
                     /locus_tag="ACHKIK_003631"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO9084729.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12478..13341
                     /gene="potB"
                     /locus_tag="ACHKIK_003632"
     CDS             12478..13341
                     /gene="potB"
                     /locus_tag="ACHKIK_003632"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO9084730.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVNELDD"
     gene            complement(13574..13723)
                     /locus_tag="ACHKIK_003633"
                     /pseudo
     CDS             complement(13574..13723)
                     /locus_tag="ACHKIK_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            13847..13932
                     /locus_tag="ACHKIK_003634"
                     /pseudo
     CDS             13847..13932
                     /locus_tag="ACHKIK_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(13955..14545)
                     /gene="espM2"
                     /locus_tag="ACHKIK_003635"
     CDS             complement(13955..14545)
                     /gene="espM2"
                     /locus_tag="ACHKIK_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HFO9084731.1"
                     /translation="MPMNTSGTSFSSFGISCHREGSFRNSFRGKNDEVVKCSIGERTI
                     SFSVRKFSRNILDTVNRQSAKDIDGWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFSLVSQENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTI
                     ANLLADKFYQKHIDSDIDIVKLRNDIPAYLTRAIQG"
BASE COUNT         3632 a         3658 c         3571 g         3738 t
ORIGIN      
        1 gaaatgaaca tccaaatgaa ctatattaaa taccgtggga taagacataa caaatgaagt
       61 ggatagtaat tgacacgata attcaacctt catgcggcat atctttttca gccatatggg
      121 gtaatatgaa aatgatcatc tggtatcaat ctactatatt tctccctcct ggcagtatat
      181 ttacaccggt taaatctgga attatcctta aagataaaga atatcctatt actatttata
      241 acatcgcacc attcaacaag gatttatgga gtctactcaa aagcagtcaa gagtgtcctc
      301 caggagaaag caaaataaca aataaatgtt tgcataatag ttgcattata aaaatatgcc
      361 catatgggct caagtaaagg gttttatata aatatgctcg atatgctatt aataatacct
      421 acataaataa aacaacgggc gtgttatacg cccgttgcaa tattaaacac atgtagtgat
      481 tacatgttct tgatgatcgc atcaccaaat tctgaacatt tcagcagttt agcgccttcc
      541 atcagacgct cgaagtcata ggttacggtc ttcgcattga ttgcgccttc cataccttta
      601 acaatcaggt ctgcggcttc agtccagccc atatggcgta acatcatctc agcggagaga
      661 ataatagagc cagggtttac tttgtcctga ccggcgtatt tcggcgcagt accgtgggtg
      721 gcttcaaaca gggcgcattc gtcaccgatg tttgctccag gggcgatacc gataccgcca
      781 acctgcgctg ccagagcgtc agaaatgtag tcaccgttca ggttcataca ggcgataaca
      841 tcatattcag ccggacgcag caggatctgt tgcaggaatg catcagcaat cacgtcttta
      901 atgacgatct ctttgccggt gttcgggttt ttaactttca gccacgggcc gccgtcgatc
      961 agttcaccgc caaactcttc acgcgccagc tggtagcccc agtctttaaa cgcgccttcg
     1021 gtgaacttca tgatgttgcc tttgtgcacc agagtcacag agtcacgatc gttagcaatt
     1081 gcgtattcga tcgctgcacg aaccagacgt ttggtgcctt cttccgaaca cggcttaata
     1141 ccgataccac aatgttccgg gaagcgaatt ttcttcaccc ccatctcttc acgcaggaat
     1201 ttaatcactt tctcggcgtc ggcagagtct gctttccatt cgatacccgc ataaatgtct
     1261 tccgagtttt cacggaagat aaccatatcg gtcagttcag ggtgtttaac cgggcttgga
     1321 gtgccttgat agtaacgtac cggacgcagg cagatgtaga gatccagttc ctggcgcagg
     1381 gcaacgttca gagagcgaat accgccacca acaggagtgg tcagcggacc tttaatggca
     1441 acgcgatatt cacgaatcag atcaagagtt tcagcaggca gccagacgtc ctgaccataa
     1501 acctgtgtgg atttttcacc ggtgtaaatt tccatccagg agattttacg ctcgccttta
     1561 taggctttct cgactgcagc gtcgaccact ttcagcatgg ctggggttac atctacaccg
     1621 attccatcac cttcaatgta agggataatc ggattttcag gaacgttgag tttgccgttt
     1681 tgcagggtga tcttcttgcc ttgtgccgga acaactactt tactttccat tcacctctcc
     1741 ttcgagcgct actggtttgc tcgtctgcca ccacgttgca tatgcgtttg cgtcctgcga
     1801 tacggatgct ttagagcaat tttttgttaa tgatttgtaa ttggcttgtc aatactaccc
     1861 tattgtttgc cgtcatgaaa gatgcgcgtt attaggctat aatgcggcaa ttcataatct
     1921 ctgaaaatac catgcaaaaa acttctttta gaaatcacca agttaagcga ttcagctcgc
     1981 aacgttctac caggcgtaaa cctgaaaacc agcccacgcg tgtgatcctg ttcaataaac
     2041 cctacgatgt tcttccgcag ttcaccgatg aagccggacg caaaacatta aaagaattca
     2101 tcccggttca gggcgtttat gcagcaggtc gtcttgaccg cgatagcgaa gggttactgg
     2161 tgctgaccaa taacggcgcg ttgcaggcgc gtttaaccca gccgggcaaa cgcaccggga
     2221 aaatctatta tgtgcaggtg gaaggtattc ccacacaaga cgcacttgaa gccttgcgca
     2281 atggcgtaac cttaaatgat ggccctaccc tgcccgcagg cgcggagctg gttgacgaac
     2341 ccgcgtggtt atggccgcgg aatccaccaa ttcgtgaacg caaaagtatt cccaccagct
     2401 ggctgaagat caccttatat gaaggacgta atcgccaggt gcgccgcatg accgcccatg
     2461 ttggtttccc cacgctgcga ctgattcgct atgcgatggg agattactct ttggataatc
     2521 ttgccaatgg cgaatggcga gaagtgacag attaaggatg caaaatgttt aaaccgcacg
     2581 ttaccgttgc ttgcgtggtg cacgcagaag gcaaattttt agtcgttgaa gagacgatta
     2641 atggtaaagc gttatggaac caacctgccg ggcatctgga agccgatgaa accttagtgg
     2701 aagccgccgc ccgtgaactg tgggaagaaa ccggcatcag cgcgcagccg caacacttta
     2761 ttcgtatgca tcagtggatt gcgccagata aaacgccgtt tttgcgtttc ctctttgcca
     2821 ttgagcttga gcaaatatgc ccgacccagc ctcatgacag cgatatcgac tgctgtcgtt
     2881 gggtcagcgc cgaagaaatt ttacaggcgt caaatcttcg ttcgccgctg gtggcggaaa
     2941 gtatccgttg ttatcaaagc gggcaacgct atccgctgga gatgattggc gattttaact
     3001 ggccttttac aaagggtgtc atctaaaagg ctgcgtgata gaatacgccg ccttgaagtt
     3061 caatgtcgtg agtgatccaa tgtctgaaac cgcaaaaaaa gtaatcgtcg gcatgtccgg
     3121 cggtgtcgat tcctccgttt ctgcctggct gttgcaacaa cagggatatc aggtcgaagg
     3181 cctgtttatg aagaactggg aagaagacga cggtgaggaa tattgcacag cggcagcgga
     3241 tctggctgat gcccaggctg tctgcgacaa gctcggcatt gaactgcaca ccgttaactt
     3301 tgctgccgag tactgggaca acgtcttcga actgttcctt gccgaatata aagccggtcg
     3361 cacgccgaat ccggatattc tgtgcaacaa agagatcaaa tttaaagcct tcctcgaatt
     3421 tgccgccgaa gatttaggtg ccgattatat cgctaccggt cattacgtac gtcgggccga
     3481 tgtcgatggc aagagccgcc tgctgcgtgg tctggacagc aataaagacc agagctactt
     3541 cctttatacg ctcagccatg agcagattgc gcaaagcctg ttcccggtcg gcgaactgga
     3601 aaaaccgcag gtgcgtaaga ttgctgaaga tcttggtctg gtcaccgcga agaaaaaaga
     3661 ctctaccggc atttgcttca ttggcgaacg taaattccgc gagttcctgg gccgttatct
     3721 cccggcgcaa ccgggcaaaa tcattaccgt cgatggcgat gaaattggtg agcaccaggg
     3781 gctgatgtat cacactctcg gtcagcgtaa aggtctgggt atcggtggca ccaaagaagg
     3841 taccgaagaa ccgtggtatg tggtggacaa agacgtcgaa aacaacattc tggttgtcgc
     3901 tcagggccat gaacacccgc ggctgatgtc tgtcgggttg attgcccagc agttgcactg
     3961 ggtcgatcgc gaaccattca ccggcactat gcgttgcacg gtaaaaaccc gctatcgcca
     4021 gaccgatatc ccttgcaccg tcaaggcgct ggacgatgat cgcattgaag tgattttcga
     4081 tgaaccggtt gccgccgtga cgccgggcca gtctgccgtc ttctataacg gtgaagtgtg
     4141 cctcggtggc ggtattattg agcagcgtct gccgctgccg gtctgattat tatctttact
     4201 taaaacaagg aagcagtgaa cgtggcaaag aattactatg acatcaccct cgccctggcc
     4261 ggtatttgtc agtcggcacg cctggtgcaa caactcgctc accaggggca ttgtgatgcc
     4321 gatgcgctac acgtctcact caacagtatt attgatatga accccagctc gacgctggcg
     4381 gtttttggcg gtagcgaagc caacctgcgc gtcgggctgg aaaccctgct cggcgtgctc
     4441 aatgccagca gtcgccaggg cttaaacgcc gaattaaccc gctacacact cagcttgatg
     4501 gtgcttgagc gcaaactctc ctcagcgaaa ggcgcgctcg acactctggg caaccggatc
     4561 aacggcctgc aacgccagct cgaacacttc gatttacagt ccgaaacgct gatgagcgcg
     4621 atggctgcta tctatgttga tgtgatcagc ccgcttggcc cgcgcattca ggtcaccggt
     4681 tcccctgctg tactgcaaag cccacaagtg caggcgaaag ttcgcgcaac cctgctggca
     4741 ggcattcgcg ccgccgtgct ctggcaccag gtcggcggcg gacgtctgca actgatgttt
     4801 tctcgtaatc gcctgaccac tcaggcaaaa caaattcttg ctcatttaac cccggagttg
     4861 tgatctatgg aattatcctc actgaccgcc gtttcccctg tcgatggacg ctacggcgat
     4921 aaagtcagcg cgctgcgcgg gattttcagc gaatatggtt tgctgaaatt ccgtgtacaa
     4981 gttgaagtac gttggctgca aaaactggcc gcgcacgcag cgatcaagga agttcctgct
     5041 tttgctgccg acgcaatcgg ttaccttgat gcaattgtcg ccaatttcag cgaagaagat
     5101 gccgcacgca tcaaaaccat cgagcgtacc actaaccacg acgttaaagc ggttgagtat
     5161 ttcctgaaag aaaaagtggc ggagatcccg gaactgcacg cggtttctga attcatccac
     5221 tttgcctgta cttcggaaga tatcaataac ctctcccacg cattaatgct gaaaaccgcg
     5281 cgtgatgaag tgatcctgcc gtactggcgt caactgattg atggcattaa agatctcgcc
     5341 gttcagtacc gcgatatccc gctgctgtct cgtacccacg gtcagccagc cacgccgtca
     5401 accatcggta aagagatggc aaacgtcgcc taccgtatgg agcgccagta ccgccagctt
     5461 aatcaggtgg agatcctcgg caaaatcaac ggcgcggtcg gtaactataa cgcccatatc
     5521 gccgcttacc cggaagttga ctggcatcag ttcagcgaag agttcgtcac ctcgctgggt
     5581 attcagtgga acccgtacac cacccagatc gaaccgcacg actacattgc cgaattgttt
     5641 gattgcgttg cgcgcttcaa caccattctg atcgactttg accgtgacgt ctggggttat
     5701 atcgccctta accacttcaa acagaaaacc attgctggtg agattggttc ttccaccatg
     5761 ccgcataaag ttaacccgat tgacttcgaa aactccgaag ggaatctggg cctttccaac
     5821 gcggtattgc agcatctggc aagcaaactg ccggtttccc gctggcagcg tgacctgacc
     5881 gactccaccg tgctgcgtaa cctcggcgtg ggtatcggtt atgcgctgat tgcgtatcaa
     5941 tccaccctga aaggcgtgag caaactggaa gtaaaccgtg accatctgct ggatgagctg
     6001 gatcacaact gggaagtgct ggctgagcca atccagacag ttatgcgtcg ctatggcatc
     6061 gaaaaaccgt acgagaagct gaaagagctg actcgcggta agcgcgttga cgccgaaggc
     6121 atgaagcagt ttatcgatgg tctggcgttg ccagaagaag agaaagcccg cctgaaagcg
     6181 atgacgccgg ctaactatat tggtcgagct atcaccatgg ttgatgagct gaaataaact
     6241 cgtatcagtg ccggatggcg atgctgtccg gcctgtttat taaaattatc cactttttct
     6301 tttttcactt tacctcccct ccccgctggt ttatttaatg tttaccccca taaacacata
     6361 atcgcgttac actattttaa taattaagac agggagaaat aaaaatgcgc gtactggttg
     6421 ttgaagacaa tgcgttgtta cgtcaccacc ttaaagttca gattcaggat gctggtcatc
     6481 aggtcgatga cgcagaagat gccaaagaag ccgattatta tctcaatgaa catttaccgg
     6541 atattgcgat tgtcgatctc ggattgcctg acgaggacgg tctgtcactg attcgccgct
     6601 ggcgtagcaa tgatgtttca ctgccgattc tggtattaac cgcccgtgaa agctggcagg
     6661 acaaagtcga agtattaagt gccggtgctg atgattacgt gactaaaccg tttcatattg
     6721 aagaggtgat ggcgcgaatg caggcattaa tgcggcgtaa tagcggcctg gcttcacagg
     6781 tcatttcgct acccccgttt caggttgatc tctctcgccg tgaattatct attaatgacg
     6841 aagtgatcaa actgaccgcg ttcgaataca ccattatgga aacgttgata cgcaataatg
     6901 gcaaagtggt gagcaaagat tcgttaatgc tccaactcta tccggatgcg gagctgcggg
     6961 aaagccatac cattgatgta ctgatgggac gtctgcgcaa aaaaattcag gcacaatatc
     7021 cgcaagaagt gattactacc gttcgcggcc agggctattt gttcgaattg cgctgatgaa
     7081 aaaattactg cgtctttttt tcccgctctc actgcgggta cgttttctgt tggcaacggc
     7141 tgcggtagta ctggtgcttt cgcttgccta cggaatggtc gcgctgatcg gttatagcgt
     7201 cagtttcgat aaaactacgt ttcggctgtt acgtggcgag agcaatctgt tctataccct
     7261 tgcgaagtgg gaaaacaata agttgcatgt cgagttaccc gaaaatatcg acaagcaaag
     7321 ccccaccatg acgctaattt atgatgagaa cgggcagctt ttatgggcgc aacgtgacgt
     7381 gccctggctg atgaagatga tccagcctga ctggctgaaa tcgaatggtt ttcatgaaat
     7441 tgaagcggat gttaacgata ccagcctctt gctgagtgga gatcattcga tacagcaaca
     7501 gttgcaggaa gtgcgggaag atgatgacga cgcggagatg acccactcgg tggcagtaaa
     7561 cgtctacccg gcaacatcgc ggatgccaaa attaaccatt gtggtggtgg ataccattcc
     7621 ggtggagcta aaaagttcct atatggtctg gagctggttt atctatgtgc tctcagccaa
     7681 tctgctgtta gtgatcccgc tgctgtgggt cgccgcctgg tggagtttac gccccatcga
     7741 agccctggca aaagaagtcc gcgaactgga agaacataac cgcgaattgc tcaatccagc
     7801 cacaacgcga gaactgacca gtctggtacg aaacctgaac cgattgttaa aaagtgaacg
     7861 cgaacgttac gacaaatacc gtacgacgct caccgacctg acccatagtc tgaaaacgcc
     7921 actggcggtg ctgcaaagta cgctgcgttc tctgcgtagt gaaaagatga gcgtcagtga
     7981 tgctgagccg gtaatgctgg agcaaatcag ccgcatttca cagcaaattg gctactacct
     8041 gcatcgtgcc agtatgcgcg gcgggacatt gctcagccgc gagctgcatc cggtcgcccc
     8101 actgctggac aatctcacct cagcgctgaa caaagtgtat caacgcaaag gggtcaatat
     8161 ctctctcgat atttcgccag agatcagctt tgtcggtgag cagaacgatt ttgtcgaggt
     8221 gatgggcaac gtgctggata atgcctgtaa atattgcctc gagtttgtcg aaatttctgc
     8281 aaggcaaacc gacgagcatc tctatattgt ggtcgaggat gatggccccg gtattccatt
     8341 aagcaagcga gaggtcattt tcgaccgtgg tcaacgggtt gatactttac gccctgggca
     8401 aggtgtaggg ctggcggtag cccgcgaaat caccgagcaa tatgagggta aaatcgtcgc
     8461 cggagagagc atgctgggcg gtgcgcggat ggaggtgatt tttggtcgcc agcattctgc
     8521 gccgaaagat gaataaatat gtccatactt cacgcattac gttaagcatc cgttataatc
     8581 ggttgcagat accagcctgt ggatgcttaa catggaatac caactcactc ttaactggcc
     8641 cgattttctt gaacgtcact ggcagaaacg cccggtggtg ttaaaacgcg gctttaataa
     8701 ttttattgac ccgatctctc cagacgagtt ggcgggtctg gcgatggaaa gcgaagttga
     8761 cagtcgactg gtcagtcacc aggatggcaa atggcaggtc agccacggcc cgttcgaaag
     8821 ctacgatcat ctcggtgaaa ccaactggtc attactggta caggcagtga accactggca
     8881 tgagccgacc gccgcgctga tgcgaccgtt ccgtgaacta ccggactggc gtattgatga
     8941 tctgatgatc tctttttctg taccgggcgg cggcgtcggc ccgcatctcg atcagtacga
     9001 cgtgtttatc attcagggta ccggtcgtcg tcgctggcga gtgggcgaaa agctgcaaat
     9061 gaaacagcac tgtccgcacc cggatctgtt acaggtcgat ccgttcgaag ccatcatcga
     9121 tgaagagctg gagcctggcg atattcttta tattccgcca ggattcccgc atgaaggcta
     9181 cgcgctggaa aatgcgatga actattccgt gggttttcgc gcgccaaata cgcgggaatt
     9241 aattagcgga tttgccgatt atgtgctgca acgtgaactg ggcggcaact actacagcga
     9301 tccggatgtt ccacctcgcg ctcatcctgc ggacgttctg ccgcaagaga tggataaact
     9361 gcgtgagatg atgctcgaat tgatcaacca gccggaacac tttaagcaat ggtttggcga
     9421 gtttatatcc cagtcacgtc atgaactgga tatcgcgccg ccggaaccgc cttatcagcc
     9481 agatgaaatc tacgatgcgc tgaaacaagg tgaagtgctg gtgcgcctgg gtggtctgcg
     9541 cgtattgcgc attggcgacg acgtgtatgc caatggtgag aagatcgatt ccccgcaccg
     9601 tccggcactg gatgcactcg ccagcaacat tgcgctgact gcggagaatt ttggcgatgc
     9661 gctggaagat ccgtcattcc tcgcgatgct cgcggcgctg gtcaatagcg ggtattggtt
     9721 cttcgaaggg taagtttgga tttaagccgg atgcggcgct gattgcgcct tatcgtgcct
     9781 acatgcactg aacgcctgta ggcacgataa gtatgtggac cagtatcaat ataacggttt
     9841 catcccggta attggcagtc cacatacgca tcgggcaatc acgacttccg ttgcgccgtt
     9901 aactcggcaa tacggacaat cacctgcacc gctttttcca taccttccag agtcacaaac
     9961 tcatgcttac catgatagtt gtaaccgcca gtgaacaggt tcgggcacgg taatcccata
    10021 aacgacaact gcgcgccgtc ggtaccgccg cggatcggtt tcagttccgg ttcaatatcg
    10081 cagtcacgca tcgcctgctg ggcgatatcg ataatatgcg gatgctcaac cactttctcg
    10141 cgcatattgt agtaactgtc ttcaatcacc agttcaatgt agcaatcagg atgtaaccct
    10201 ttgcccactt ttttggcgat ctccatcatt ttacgtttac gcgcttcaaa ctgtttacgg
    10261 tcgaaatcac ggatgatgta gtgcatatcg gctcggtcaa cggtgccttt catgctcgcc
    10321 aggtgataga aaccttcata gccttctgtc atttccgggc tttcatccgc cggaacttcc
    10381 gcatgaatac gtgccgccag cgacagcgca tttaccatca ctcctttcgc cgtgcccgga
    10441 tgaacattgt taccgacaat tttgatattg accgatgcgg cgttgaagtt ttcaaactcc
    10501 agttcgccta cgccaccacc gtcaacagtg taagcccagc gggcatcgaa ggcatcaaca
    10561 tcaaaatgtt tcgccccttt gcccacttct tcatccgggg taaaggcgac gcgaatatca
    10621 ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg tcatgatttc tgcaatacct
    10681 gctttgtcat cggcacctaa caaggtttta ccatcggtgg taatcaacgt ctgacccagt
    10741 agctgatgca gcaccgggaa cataaccggt gataaaactt catcgccgat acccagcgca
    10801 atatcgccac cgcgatagtt ttcaacaatt tgcggattca catttttgcc gctgcaatcc
    10861 ggtgaggtat ccacatgaga aataaagcca atcgccggga tatcgccagg gacgttagcc
    10921 ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca cattgataag ccccatctct
    10981 tcgagctgct ctttcagcag atgcaataac ttccattggc cttccgtgct gggaacctgt
    11041 ctcacccctg cttttgattg ggtatccaga gacacgtagt tcaaaaatcg ctcaagtagt
    11101 ttatccatgt agtcaccctc actttttgtg acaacattat taagaagcaa gaaaagacaa
    11161 atattgcgtc aggtcacttt tacccctgca agcgggaata tttatcagca ttaccttaat
    11221 gaatataaag ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta
    11281 gaattaccgc cctcattaag agtcaccaag gtggttaacc acaaaccccg catcggtaag
    11341 ccatccgttg cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt
    11401 caccgctggt gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc
    11461 agctggatct gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg
    11521 gtaaaacaac cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca
    11581 tgctggataa cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt
    11641 tccaaagcta cgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc
    11701 gcatgcaaaa aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg
    11761 tgcaactgga aaccttcgct caacgtaaac cgcatcagct ctctggtggt caacagcaac
    11821 gcgtcgccat tgctcgcgcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc
    11881 tctcagcgct ggattacaaa ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc
    11941 gtaagcttgg tattaccttc gtctttgtca ctcacgatca ggaagaagcg ctcaccatgt
    12001 cagacaggat tgtggtgatg cgcgagggtc gtattgagca agatggtaca ccgcgcgaaa
    12061 tttacgaaga gccgaaaaac ctgtttgttg ccggcttcat tggcgaaatc aatatgttta
    12121 acgccactgt catcgaacgt ctagacgagc agcgcgtacg cgccaacgtt gaaggccgcg
    12181 aatgtaatat ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc
    12241 gcccggaaga cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg
    12301 gttacgttcg tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa
    12361 atggcaagat ggtgatggtc agcgaattct tcaatgaaga cgatcctgac tttgaccact
    12421 ctctcgacca aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg
    12481 aagaacacaa gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg
    12541 tttgtctttc tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc
    12601 agtttcgtaa aaatggtctt tacgctggat aactacacgc gtctgctcga tccgctctat
    12661 tttgaagtgc tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg
    12721 ctgggctacc cgtttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg
    12781 ctgtttctgc tgattgttcc cttctggacc aactcattga ttcgtatcta cgggctgaaa
    12841 attttcctca gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac
    12901 acaccaatcc gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg
    12961 ctgccgttta tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt
    13021 gaggcggcgc gcgatctcgg tgccagcaag ttacagactt ttatccgtat cattattcca
    13081 ctgacgatgc cgggaattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg
    13141 ttctatgtat ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag
    13201 gtacagttcc tcaatattcg tgactggcca tttggtgcag ccaccagcat tacgctgact
    13261 atcgtaatgg gcctgatgtt gctggtttac tggcgcgctt ctcgtctgct gaataagaag
    13321 gtgaacgaat tagatgatta atcctttata ttcaatttat taggtttgtt tttgagtgat
    13381 tttgtcgttt aaggtaacgg cgttatagat cctatcaaaa tcaatcaatt gcattctggt
    13441 tttggagaaa gatttttcga gagcttcggt ctttaactaa aactgattgc gtataatcgc
    13501 aataccccct ccccgactat ctgaatggtc ggggaggcac ttcaagcaca aaatatcatc
    13561 cctgctccgt tgattactct acaggtgaag tcggccactc aatatcaggt gctgctgatg
    13621 tatcaacacg attcaacaac acccgatact ttttccaggc ttcccgcaat gaagtctctt
    13681 cctcaattac gatctccaga tctgcagcat cctgaagtgg ggcgcaccac gcaaacatca
    13741 caaaatattg tcgttgtgat ggacctgtgc aatagtgaat cacgctacgt ttctgtgtac
    13801 aacgtactgt agttttcttt acgatttata gctgtactgg tgagccatga gtaatctgaa
    13861 tccatgcatg gcgtgtggtg cctgttgtgc atttttctgc gtctctttta ctggtccgaa
    13921 gccgatgatg ctgaagcagt cctctcctct agcctcatcc ctgtatagca cgcgtcaagt
    13981 aagctgggat atcatttcgt agttttacaa tatcaatatc cgaatcgata tgtttctggt
    14041 aaaacttgtc cgcaagcaag tttgctatgg tatctgatgt acgatttttt acatcccggc
    14101 tcataccatc acaaagagca tccattttct tgccaaaaga agcactcccc attattacat
    14161 gattaataga actttgtgct gccttaacat caagggttag ttgattctcc tggctcacaa
    14221 gagaaaagac tctttgcctc tcttcagtgg aaatttttgc atttttttga aaacagtaat
    14281 tatcaatctc ctggttgatc acccttgagg gatatactat gcgttcatct tttatccacc
    14341 catcaatatc tttagcactc tgcctgttca ctgtatccag tatatttctg ctaaatttgc
    14401 gaacagaaaa gctaatagtc cgttctccaa ttgaacattt tacgacctca tcattcttcc
    14461 ctcggaaaga gttcctaaag gagccttctc tatggcaact tataccaaaa gagctgaaag
    14521 acgtacctga agtattcatc ggcattataa ttccttttta taaaaaagcg tttacaagat
    14581 tgcatgcttg tgtttattg
//
LOCUS       DAZGJW010000118        14559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZGJW010000118 DAZGJW010000000
VERSION     DAZGJW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.118"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            90..446
                     /locus_tag="ACHKIK_003636"
     CDS             90..446
                     /locus_tag="ACHKIK_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO9084732.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(497..1393)
                     /gene="yhaJ"
                     /locus_tag="ACHKIK_003637"
     CDS             complement(497..1393)
                     /gene="yhaJ"
                     /locus_tag="ACHKIK_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO9084733.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1498..2199
                     /locus_tag="ACHKIK_003638"
     CDS             1498..2199
                     /locus_tag="ACHKIK_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO9084734.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPDAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2222..2386
                     /gene="yhaL"
                     /locus_tag="ACHKIK_003639"
     CDS             2222..2386
                     /gene="yhaL"
                     /locus_tag="ACHKIK_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO9084735.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2598..3908)
                     /gene="cyuA"
                     /locus_tag="ACHKIK_003640"
     CDS             complement(2598..3908)
                     /gene="cyuA"
                     /locus_tag="ACHKIK_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO9084736.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3936..5267)
                     /locus_tag="ACHKIK_003641"
     CDS             complement(3936..5267)
                     /locus_tag="ACHKIK_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9084737.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5542..6906)
                     /gene="tdcG"
                     /locus_tag="ACHKIK_003642"
     CDS             complement(5542..6906)
                     /gene="tdcG"
                     /locus_tag="ACHKIK_003642"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO9084738.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6978..7367)
                     /locus_tag="ACHKIK_003643"
     CDS             complement(6978..7367)
                     /locus_tag="ACHKIK_003643"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO9084739.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7381..9675)
                     /gene="tdcE"
                     /locus_tag="ACHKIK_003644"
     CDS             complement(7381..9675)
                     /gene="tdcE"
                     /locus_tag="ACHKIK_003644"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO9084740.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVTTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9709..10917)
                     /gene="tdcD"
                     /locus_tag="ACHKIK_003645"
     CDS             complement(9709..10917)
                     /gene="tdcD"
                     /locus_tag="ACHKIK_003645"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO9084741.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGSSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10943..12274)
                     /gene="tdcC"
                     /locus_tag="ACHKIK_003646"
     CDS             complement(10943..12274)
                     /gene="tdcC"
                     /locus_tag="ACHKIK_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO9084742.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12296..13285)
                     /gene="tdcB"
                     /locus_tag="ACHKIK_003647"
     CDS             complement(12296..13285)
                     /gene="tdcB"
                     /locus_tag="ACHKIK_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO9084743.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13384..14322)
                     /gene="tdcA"
                     /locus_tag="ACHKIK_003648"
     CDS             complement(13384..14322)
                     /gene="tdcA"
                     /locus_tag="ACHKIK_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO9084744.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAVV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3793 a         3892 c         3389 g         3485 t
ORIGIN      
        1 tttataacat ccgaaaaaga aagatacgaa tagcatttac acaatacccg gaatagactt
       61 tcgtcacttt taattaaagg gatgttttta tgcagtggta tttagccgta ttaaaaaatt
      121 atgttggttt ctctggtcgc gcacgtcgta aagagtactg gatgtttact ctgattaacg
      181 ccatcgtcgg cgctattatc aatgtcattc aattgatttt aggtttagag tttccattcc
      241 tttctctcat ttacctggca gcaacaatca ttccggtcat tgctctgtgt gttcgtcgtc
      301 tgcacgatac tgaccgttca ggtgcgtggg cgcttctcta tctggtaccg atcattggct
      361 ggttagttct gtttgtattt gcctgtttag aaggcaattc tggcagcaat cgttacggaa
      421 acgatcctaa gtttggttca aattaatctt agaattgggg cgacatttcg ccccttttta
      481 ttaataataa caaatattat tttccgctaa aaagtttggg aatttcccgc agacaccagg
      541 atttcgcttc ccccatactg tcacgacgcc aggccataat aatatcgatc tcgctggtcg
      601 attccgggct gacgacacgc aaccgccctt ccgcaatatc tttttcgacc atcggatacg
      661 gcatcgtcgc cacgccaagc cccgccagta atgcctgacg tttatcttca atcgtgctca
      721 ccgttaagcg cggctgtttg tccagcagct gtacggtcaa caccgggcgc tcacgagcgg
      781 tatccgctac cgcaattcca cgatatttca cgcgcgtcac ttcagataac ggttccggct
      841 cctgatgaat cgggtgatca ggcgcggcaa cgtagacgtt cattaacgta tagagcttgc
      901 gcgagttgat ctccgacgag gaacgaaaat gcatatccgg cgcgataaca atatccgccc
      961 gcccctgctc cagtcgttcc cacgcccccg ccagcacttc ggtgatgatt gccagttggg
     1021 tattggcttt tgccgccagt ttgtcgatta acgggaaaaa ggcaggtgtc ggtaccagcg
     1081 cttcggtcac aatggtgaga tgcgtttccc aaccgcgcgc gagggcttcc gcatcggtag
     1141 tcagtttatc tgcggcttcc agcaaaacgc gccctcgctc cagcaacatc ctcccgacat
     1201 tggtgaattt ggtgcgatgg cccgagcggt caaacagcac cacatccagc tcttcttcca
     1261 gtttttgcat ggtgtagcta agtgcagaag gcacgcgtcc cagctcatcc gccgccgccg
     1321 caaaactgcc ccggcgatcg atcgcatcca taacccgtag tgcttccagc gttaatgccc
     1381 tttctttggc catttcgttc tcattcagga aatttgaaca taccgggcag aatatctggc
     1441 taacaatgta gcgtccagcc ccttaccata aaaggaagta aagagaggtc aagaattatg
     1501 attactaccc gaactgccag gcagtgtgga caagcagact acggatggtt gcaggcccgg
     1561 tatacttttt cctttggaca ctacttcgac ccgaaattgt taggctatgc ctccctgcgt
     1621 gtgcttaacc aggaagtgct ggccccagat gccgcctttc agccgcgaac ctatcccaaa
     1681 gtcgatattt taaatgtgat tctggatgga gaagcagagt atcgcgacag cgaaggcaat
     1741 catgttcagg ccagcgccgg tgaggcgttg ctgctctcta cccagccggg tgtcagctac
     1801 agcgaacaca atctcagcaa agacaaaccg ttaacacgaa tgcagctttg gctggacgcc
     1861 tgcccgcagc gagagaatcc gctgattcaa aaactggcgc ttaatatggg caagcagcaa
     1921 ttaatcgcct cgccagaggg tacgatggga agcctgcaat tacgccagca agtgtggctg
     1981 caccatatcg tgctcgacaa aggcgaaagt gcgaatttcc agttgcatgg gccacgcgcg
     2041 tatttgcaat cgattcacgg gaaatttcat gcgcttacgc atcatgaaga gaaagcggcg
     2101 ctgacctgcg gtgatggggc gtttattcgt gacgaggcta acattacgct ggttgccgat
     2161 tccccactgc gcgctttgct gatagatttg cctgtctagt tgtttttaca gggagatgat
     2221 gatgagtaaa aaatcggcca aaaagcgcca gccagtgaag cccgtggtgg cgaaagaacc
     2281 tgctcgcacc gccaaaaatt ttggctatga agagatgttg agcgagctgg aagccatcgt
     2341 cgcggatgct gaaacgcgtt tagccgagga tgaagctacc gcgtaaagac aggttgccgg
     2401 atgcggcgaa aacgccttat ccggcctaca aaatccaaca acattaacga attaaacaac
     2461 ccgtaggccg gataagatgc gccagcatcg catccggcat caggtacaaa acacaacaac
     2521 atcaacaagt tgagcacccc gtaggccgga taaggtgcgc cagcatcgca tccggcacga
     2581 tacccaaaac ctggcgttta tctggccttg ctcgccataa tctcgataat ctgccgatcc
     2641 gtttgctgca tcgaatggct tgctaacgca cacaggttgg caatcgactg ctcaacatca
     2701 tgcgccacga tcccttcatt gccggtcacg gcggtatcat ccagcgccat taacaccgct
     2761 ttccacgcag ccgaagcact ggtcgaaacc ttcatcgcgc agctgttcga cgcaccatcg
     2821 caaatcatgc cgctgacatc gccgatcata ctgctgatcg ccatcgagat ggtttcataa
     2881 cgcccatcca ccagccatgc catcccggcg gcggccccca ttgctgcggt cgttgcggca
     2941 cacagcgcag acaaacgcgg taactggtta tggatgtaaa ttgcgctcaa atgagaaagc
     3001 atcagcgcac gcgccagccg ttcatcatcc gctccgaagt gttctgctac caccaccaca
     3061 ggcattgttg cggtgatccc ctggttaccc gagccggagt tactcatagc cggaagcgta
     3121 gcgccgccca tacgcgcatc ggatgccgcg ctggtacgaa tcacaatgga tgaagagaga
     3181 tctttcgcca gcaaaccgcg ctcgcactgt ttttccagcg tcgcgccaat atgcagcccc
     3241 cacttaccgc tcaaaccttc ctgcgataac gcacaattta gcttcgcgga atcgagaata
     3301 aagcggatcg ccgcaaacgg gacttcattg acgaacttca ggatctcagc cagcgtcgtt
     3361 ctggaaagca ccgtcagcgg agactcttgc tcgccctctg ccacacacgc ctgctgggta
     3421 aacaccacac cattgtgcgt ctcaatatgc acaatgttgg tatgcccgcc gacgatggtg
     3481 acacacgccc acttctcacc gttccagact ttggcgcgtg agaagaggat ttcattgcaa
     3541 ggttcctgga tcttaacgga gactttcccc gccgccagca gtgctttggc atcggcaatt
     3601 gcctgcgcag ttgcgtcttt cagcacttcc agcccggcgt tggcatttcc acctaacgcc
     3661 cccagcgccg ccgcaatcgg cagccccacc attcccgtgc cgggaacggt gacgcccaga
     3721 ccgttcttca tcagatttgg cgaaacccag gcttctacac gttcaaccgg accttccagt
     3781 tctgccgcag caaccgccgc cgccagcgcc agtgaaatcg gttcagtaca tcccagcgcc
     3841 ggttttactt cctcctgaac ggcgaggatg taacgctgcc ataacggatt taaagtcgaa
     3901 tcaaacatat taaaaacctt aaaatttcag gtaaatcaag aaaatgccag gaacggagaa
     3961 acacaaagca acaaaccagt gacgataatc aggtacagag acatcccttt gtatttgtgc
     4021 aatgccggta ctttgtaaac cagccacgcc gggatcaggc accctaccat gccgaaaatc
     4081 gggctacaga tagaggtgaa gctcaacacc ggtgcgttca gtacgatggc gctccaggcc
     4141 agcaaaatgg cgaaaatcat gatgccgcgc tgaacaagat tttcgttaat cttctcggca
     4201 ggcatcttgc gacgcaggat gttcattacg atcccttgcg ttgcttcgcg aaagcctaaa
     4261 tagacgccaa agaatgcagt catgacagca aagatattga gaatgacgct gaccactttc
     4321 acccacgctg cgccgtcacc gctaataaac tgtgcggcaa tcgccagcgc ggaaatattc
     4381 tgctcatagg cttttaccgc ttcgtcatgt cccatcgcca gcgtgaacga cacggcgtag
     4441 aaaaagacgg tgacaaacaa aatgccaaac gcgatattca ttgcccgcaa tgctttatgc
     4501 cgcgccactt caattgattt ttcccgcgag cgataagaga tcaccatcgg gcttaacgtc
     4561 tggataaaca gaatcgacgt cagggtaaac ggcagcgtaa taatggcgtt tttcaccagc
     4621 agccccagcg gcggtagcga accgacgtta tacagatgcc acattcccac catcgacacg
     4681 cccagcgccg cgaccaccag cagcttggtc agcaccatgc cggtcgaaat tttgaataac
     4741 aatttctcgc cgcgtgaaga gatcgccacc agaatgcaaa tcagtaccag accataaaag
     4801 ggactgtctg acagcaaccc ttccgtcacg ccgaaggtat gcagatagga agcactatcg
     4861 ttggtgatgg cggtggaata gacgaacatc caaatcacca gcattacgaa atagagcgca
     4921 cctaacagga tgccccagtt tttacctaaa taaccgctaa tgacactcgg gtaatcttta
     4981 cattctggtg attctgccag cgtattaata aacaaccgct gaaacagata cattgccggg
     5041 taaccaatca ccgatgagag caaaaatacc cacaatccca tcaaaccgac ctgcaccggg
     5101 agaaaaacaa tccccgcgcc aatcgccatc ccgatactca taatcaccca gccggtgtca
     5161 gtactgtcga atttgatcgc ttctcgccac tcgctctcac tcattccagc acgacccgcc
     5221 ggggtcgaag cgtctgcaat gacgccttta ttcgatgcaa tttccataat ttctcgctca
     5281 atattttgta gggcttatta tttttttccg agccgcatca aggcgatatg cggtctacgt
     5341 ttttgcaggc gagttattag aataaagaaa tgatacgcgc gaggtcagag aaaatcttca
     5401 caatcaagac tttgaaataa cggagatgga taagaatttt ctactaaatt aatcgcaaga
     5461 gaatattcat tctctatttg cggcagatca caaaaaaaag gtgcacattt gtgcacccaa
     5521 ggatgaaagc tgacagcaat gtcagccgca gaccacttta atggccagtc ctccgcgtga
     5581 tgtttcgcgg tatttatcgt tcatatcttt gccggtttca tacatcgtct cgatcacttt
     5641 atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc atccgcgcgg cgtttactgc
     5701 tttcacggca ttaatggcat tacgttcaat gcacgggatt tgtacctgtc cggcaaccgg
     5761 atcgcaggtc agcccaaggt tatgctccat cgcgatttcc gccgcattgc atacctgcgc
     5821 cggactgccg cccagtagtt cagttaaccc tgccgccgcc attgaacagg ccacgccaat
     5881 ctccccctga cagccgacct ccgcgccaga gatggaggcg ttcattttat acagcgcgcc
     5941 aatagccccc gctgccagaa aatagcgggc aattgaccgc tcgtttaccg gacgacggaa
     6001 cttatcgtaa taagccagca ctgccggaat aatgccgcac gcaccgttag tcggtgccgt
     6061 taccacgcgc ccgccagctg cgttttcttc actaaccgcc agcgcgtaca tgttgatcca
     6121 gtcgatgaca ttcatcggat cgttagagat gttatcgctg gaaaccagct gacgacgcag
     6181 cgctacggca cgacgcggca cattgagcgg accaggcagc acgccttcag tgttcatccc
     6241 gcgttcaata ccgtcatgca tcacttgcca gatacgggca aaccctgcgt caatttccgc
     6301 tttgctgcgc agcgccagct cgttgtgcat catcaggcca gatatagaca ggccgttgta
     6361 atcacacatt ttcagcagtt cacctgctga gtggaaatcg taaggtacgg acgtttcgac
     6421 atcgtgcgac aggccgaagt gttcttcttc gacaataaac ccgccgccga cagagtaata
     6481 ggttttactt aatagcgctt cctgtccctt ccaggcagtg atccgcattc cgttctcatg
     6541 gcgaggcaac atttcgggat ggaagataat gttctttgct acaggaaaat caacaatatg
     6601 cgcaccagat gccactggca gccgcccgct gcgcgttact aactctataa atgcagggat
     6661 ctcatcaatg acaacatcct gcggactgtt tcctgccagt cccatgataa tggcgacatc
     6721 cgtggcatgg cctttgcccg tcagtgacaa cgacccatac agatcgacca caatatggct
     6781 cgtcgcggtt aataagccgc tactttccag ccgatcaata aaactttttc cggcattcat
     6841 tggccccacg gtatgcgaac tggagggacc aatcccaatt ttgaaaatat cgaatgcact
     6901 aatcatatcc acaccctcgg attgccgttc agtgaagtgg agcggaacga ccttgcgacc
     6961 gtcccgctca cgaggcttta cgcactacgt actgcgatag cttcaatttc cagcttcaca
     7021 tctttcggca aacgcgcaac ctgcacacag ctccgggtcg ggtaggtcgc ctgatgctca
     7081 tcgaagaact gcttatagac ttcattgatg gtggcaaaat cattcagatc ggtgatgaac
     7141 acggtcatct tgatgatatc gcccacgctc agcccggcag caaccacgat cgctttgacg
     7201 ttttcgaggc ttaaacgcgc ctgatcttgc acatcagccg ggatctcacc ggtctgcggg
     7261 caaaccggaa tttgcccgga ggtgaagacc atgctgccta aatcaacgcc ctgaacataa
     7321 gggccgattg cgcctggggc acgttgcgtt tcgataatct ttttcataca tcctccggcg
     7381 tcagagcgcc tgggtaaaag tacgtgaaat aacatcctgt tgctgttcac gggtcagtgc
     7441 gttgaagcgc acggcgtagc cagagacacg gattgtcagg ttaggatatt tttccgggtg
     7501 ctcgatggca tccagcagca tttcccgatt cattacgttg acgttgaggt gttgaccgcc
     7561 ttcgacatcc gcttcgtggt ggaaataccc atccagcagg ccgacaaggt tggttttacg
     7621 tactggatct tctttgccca gcgccgcagg aacgattgag aaggtgtacg agatcccatc
     7681 tttggcgtag gtgaacggca gtttcgccac cgacgtcaat gaggccacgg cacctttgcg
     7741 gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc gacgaccgtc
     7801 cggcgtatta ccggttttct ggccgtacac cacgttagaa gtgatagtca gaatcgactg
     7861 ggtagtgacg gcgttgcgat aggttggcag cgctttaatt ttcttcataa agcgttcaac
     7921 caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg ccgtactgcg gatattcacc
     7981 gtcgatttca aagtccaccg ccaggccgtt ttcgtcacgg attggtttca cgcgggcata
     8041 tttgatggca gacagggagt ccgtcgccac cgacaggccc gcgatgccgc atgccatagt
     8101 gcgatagaca tcacgatcgt gcagcgccat cagcgaagct tcgtagctgt acttgtcgtg
     8161 catgtagtga atgatattca gcgcgctgat gtactgcacc gccagccagt ccatgaagtg
     8221 atcgaggctg tccatcactt tgtcgtaatc cagcacgtcg tccatcagcg gtgctgtttt
     8281 cggcccgacc tgaatcttca gcttctcgtc caccccgccg ttaattgcgt agagcagcgt
     8341 tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc ttaccaatca ccattgggct
     8401 gacgcagcag gcaatcgcgt aatcgtcgct gttgaagtca gtacgcatca gatcgtcatt
     8461 ttcatactgc aaggaagagg tgacgatcga cacctgcgcg gcatattttt tgaaggcaat
     8521 cggtaattct tccgaccaaa gaatggtcag gttaggttcc ggtgccggcc ccatagtgtg
     8581 cagggtgtgc aaatagcgga aggagttttt ggtcaccagc gtacgaccgt ccagccccat
     8641 cccgccgatc acttccgtcg cccagattgg gtcgccggag aacagcgaat caaattccgg
     8701 tgtacgcagg aagcgtacca tacggatctt catgatgaag tgatcgatca gttcctgtgc
     8761 ctgctgctca ttgagtacgc cagctttaaa gtcgcgctca atgtagatat cgaggaacga
     8821 tgccgtgcgg cccagcgaca tcgcgccgcc attttgcgat ttcactgccg ccagataagc
     8881 gaagtagagc cactgcaccg cttcctgcgc attctgcgcc gggcgagaga tatcaaagcc
     8941 atatttcgcc gccatttcct gaatctgcaa cagcgcatga cgatgctctg ccagctcctc
     9001 acgcagacgg atggtggctt ccagatcctc gcctttttcc agacgagact ggagatcggc
     9061 aaattgcagt tcgcgttcac gtaccagata actgatgcca tacagcgcta cgcggcgata
     9121 gtcaccgata atgcgcccac ggccatagcc atctggtaaa ccggtcagca cgccagattt
     9181 acggcagcgc agcatatccg gtgagtaaac atcaaatacg ccctggttat gggttttacg
     9241 cagatcggta aacagatatt caaattcact gtccatttca cggccatagg cgtggaatga
     9301 acttttaatc atattaatgc caccgaacgg gtgtagcgca cgtttcaacg gcgcatccgt
     9361 ttgcaggcca acaatttttt ccagcggctg gttaatatat cccgcatcat gagcggtaat
     9421 tgtggtggca atattggtat cgaaatcaac cggcgcgtgg gttgcgtttt cgatacggat
     9481 accttccatt actttttccc acaattccgt ggtggcaggc gtcgcttcgg cgaggaaaga
     9541 ttcatcgcct tcatacggtg tatagttatg ttgaataaaa tcgcggacat taatttcgtt
     9601 tttccagtcc gtacctttaa agccaagcca tgcgtcggcg tacagcttat cgctggtatc
     9661 aatatctacc ttcatgaaaa ataatctctc tacaatactt caactaaatt atgcaaattc
     9721 tgcgggcgcg ttaactttgc ctaaatgaat ggcatccaaa gcaatcattt tttcttcgtt
     9781 agtcggaata acagcgcaaa tgacacgcgc attttcactg gaaacaattc gctcaccaca
     9841 ggagttagag cgattattca tttctgtatc aatctctacg cctaatacag ccaaatgttc
     9901 catgaccaga cgacgaatta agcttgaatt ctctcctatt ccgccggtga atataattcc
     9961 atccaggcga cgtaatgaag ctgcgtgtcc ggcaatatga cgggcaattc ggtgaacaaa
    10021 ggttttaatt gccagttgcg cgcgttcgtg accttcatgc caggcttttt ccagaacacg
    10081 taaatccgaa gaaagaccgg aaatacctaa taatcccgac tctttattca ctacgcgttc
    10141 caggtcaccc aggctctggt tggtttggct ggcgacccag gacatcgcac caaagtcgac
    10201 atcgccactg cgggtaccca tcatcaagcc ttccagcggc gtcattccca ttgaagtatc
    10261 aacactctga ccgttgcgaa ccgcgcagat tgacgcgcca ttgccaagat gcgccacaac
    10321 caggccggag tcatcttccg ccagattcag cagcgaatgt gcgcgctggg aaacatagcg
    10381 gtgcgacgag ccgtggaaac cataacggcg tacacctaac tcttcataat atttccacgg
    10441 caggccgtat aaataagctt ccggagccat cgtctggtgg aaactggtat caaataccgc
    10501 cacctgagtt acgcccggaa ataattgctg cgccgattca ataccactta aattggcgta
    10561 attatgcagg ggtgccagtg gagaaacgcg acggatatta tcaatgactt catcggtaat
    10621 aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg cggtggccaa ttaaggccac
    10681 actgtcattt aaattccgtt tttccagttc aaatgcaatt gccttcaatg caccttcgta
    10741 gctgtggtga gccagcggtg ctggctctcc cccatttacg gataagaatg cattttccga
    10801 gttaataccg tcggcaatac ctgacattaa tacttcacag tcgctggcat cgagtacgga
    10861 aaacttaatc gaagacgaac cacagttaat aaccaaaaca accggaaatt cattcatctc
    10921 ttttctcatc ctgagttacg gattaaaaca gtttgtatac gatgttcagg atggtcagca
    10981 gaccaatcac ggtaacaaac acgttatcca gacgaccacg gtatttcgcc agagacggcg
    11041 ctttacggat ggcatacatc ggcaacaggc acagcaggga tgcgataatc ggtgcgccca
    11101 tggcttcaat aaggtcgagg atgttcgggt tggcgtaggc aacaacccag gtggagccca
    11161 tgatgaagat catgctgata gtgttcagtt tacccagcga cactttagtt ttgtcgcctt
    11221 tataaccgaa cttcagaatc agaccattca agccttccag cgtccccaga tagtgaccga
    11281 agaaagattt gaagatagcc acgagtgcga tgatggaagc cgcatattcc agtgtaatcg
    11341 cgaacgttgt tttggtaccg gtcatggacg caaagtggtt agccagataa gaaagcactg
    11401 gaatattctg cgctttggct tccgccatgt tggccggaga cagcgtaaac aggcagctaa
    11461 aggcaaagaa catcaccact gcaaccatca gcatgctggc acgagaaatg atttgggaac
    11521 atttacgttc ggtgaagtcg cgaccgaagt ctttctcata ctcttcacgt ttagaaacca
    11581 cgaaggaaga gacgattggc gagaagttaa aggagaaaac catgatggaa atccccagcc
    11641 agacagtgat caggataccg tcatgaccgg ttaacgacag cgaaccgagg tcaacctggt
    11701 cgataactgc agagttccag taagggatca gcgacagaga aatcagcacc aggctggcga
    11761 taaacggcca taccaggtag ctcatcactt taaccatcag atccttacca aaccagatga
    11821 cgaaagccat cagcagcaac aggaacagcg ccacaaagcc gcgattcagc ggtgcaaatc
    11881 cgagctggtt ttcccagaac gtcataaagg tattggtaat agtaacgcca taaatccaca
    11941 gcagtgggca aatcgcgaag aagtacagga acgtgataac cacgccgcca gttttaccaa
    12001 aatgctcttc caccgtttcc gtaatgttgc cggaagggtt agagccagaa agacacagac
    12061 gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc caataccaac atcagaagaa
    12121 tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa cagcaccccg gcgccgattg
    12181 ccgtaccaaa caagcctaac gtccatgtgg tatctgattt acgccaggac gattgttttg
    12241 tctggctgga tacaatgcta tctgaagtac tcatatccta tcctcaacga attaattaag
    12301 cgtcaacgaa accggtgatt tgagagacgc gagaaagatc gatattgccg ccggaaataa
    12361 tactgacggt ttttctgttt tgaatatatt ggtctaattt accgcttaat aatgcagcac
    12421 atgccagagc gcctgcgcct tcggtgacga ctttattgcg ctgaattaag gcaatcatac
    12481 tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc gactaattca cgaacgattt
    12541 cgtaagttaa attacccggg cgggagacat cacaaccatc cgccagggtg ccggtagttc
    12601 ggtgcgtggt tatttctccg gagtggaaag aagccgccat gccgtgaacg ttttcagact
    12661 gtacgccaat aacacgaatg gtcgggttaa tagatttaat tgccaccgca ataccagcaa
    12721 ttaaaccgcc accaccaatt ggcacaatca cgttatcgac atcatagaga tcttccataa
    12781 tttccagacc aatcgttccc tggccagcaa tcactttcgg atcatcgtaa ggtgggataa
    12841 aaatacggcc ttccatttcg acaatttcgc tcactttagc gatagtgtcg ttgaaattat
    12901 caccatgcag aacgacttct gcggagtagt cgcacgttgc cgctactttg gattttggcg
    12961 caccttttgg catcaccact ttaccgtcga tacccagcat cgcgcaggag agcgaaaccc
    13021 cttgcgcatg gttgcccgca gaacaggcca ctacaccttt acgtttttcc gcatcagtca
    13081 gtgaacttaa tttattaaat gcgccacgaa ttttaaatga acccgtacgc tgcatatttt
    13141 caaacttcag gaatatttca cctttgcaac gttcactaaa atagtttgag cgaggcatgc
    13201 ctgttttata aattcgccca gccagtcgtt gtttcgcttc aataatgtca tcaatagcaa
    13261 ccggcagatc gtatgtaata tgcattataa aacctcttcg cctgaattaa atatgtaggt
    13321 aaccgtaacc gacacctgca aaacagacag gtggattatt tatgtttaaa taaaaacaaa
    13381 taactaacca acttctatta attgccttcg tctacaccca ttataagatg aatactcttt
    13441 ggctaattcc accaaaaccg atgctgcttt tttaatacga taatttttcg accataccac
    13501 ggcatattgt gccacaggta atgtttcttc aaccggaata gtaataaatt gatttgaacc
    13561 aaaaggtgac gtcatatcac aaggaattac agttaagaaa tcagcattga gaacaagatt
    13621 ataaattgtc acgactgagt cggttttaac gatgttttca atactgatgc catttctttg
    13681 taacgtagta agaagctcgc tgtagtaccc catattggtt tgtggcaaca cccactgttc
    13741 gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt cgggacttac tggctaccag
    13801 cacaaactcg gactcgaaca gcggctcaac atgtaaatcc tgaagcttca tttctgcact
    13861 taacgtacca atcgcaaaat ccagtcgacc gtcgcggatt gccggtaaga acgaagacag
    13921 ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc tctttgaact tgttgatcat
    13981 ccctgacata aaagtaaaac caatcaatga aggaaaacca aatgagactt ccaccaccgc
    14041 ctcagaagac ataccgctta tctcattaac catatttttc atttcacggg taatggattc
    14101 ggaacgggag agtaacaatt gaccggcagg tgttaatgtt acaccggtat ttttccgcac
    14161 cactaattcc acaccaaaat aatcttcaat atcgttaatg attttactga cggccggttg
    14221 agttaaccct aattcttttg cagccgagcc gatagagcca cttctaatga cttcctgaaa
    14281 gactaccagg tgctgcgttt tcggaagaag aatagtgctc ataacgacct acgttaatta
    14341 cctcattgac ggcatgaagt gtatcaaaat gaaatgaaca ggatatgtgc gaccactcac
    14401 aaattaactt tcaatacttt ccatggtatc gttacgcaaa ttaaatacct ttaaatatca
    14461 acaagttaaa gtataaaaat cagcataaac cctgattttt gtcaaaaaaa atgacgggga
    14521 taaccatatt ttatggcgat aacatcattc gttatgtgg
//
LOCUS       DAZGJW010000119        14397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZGJW010000119 DAZGJW010000000
VERSION     DAZGJW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.119"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            6..572
                     /locus_tag="ACHKIK_003649"
     CDS             6..572
                     /locus_tag="ACHKIK_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9084745.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            623..3082
                     /gene="ybgQ"
                     /locus_tag="ACHKIK_003650"
     CDS             623..3082
                     /gene="ybgQ"
                     /locus_tag="ACHKIK_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO9084746.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNDVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3097..3828
                     /gene="ybgP"
                     /locus_tag="ACHKIK_003651"
     CDS             3097..3828
                     /gene="ybgP"
                     /locus_tag="ACHKIK_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9084747.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNIGNYSGSQFYLGYMD
                     DYGALRMTTLNYSGQCHLQAVEAKK"
     gene            3825..4886
                     /gene="ybgO"
                     /locus_tag="ACHKIK_003652"
     CDS             3825..4886
                     /gene="ybgO"
                     /locus_tag="ACHKIK_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9084748.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5038..6084
                     /gene="abrB"
                     /locus_tag="ACHKIK_003653"
     CDS             5038..6084
                     /gene="abrB"
                     /locus_tag="ACHKIK_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO9084749.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6081..6872)
                     /gene="nei"
                     /locus_tag="ACHKIK_003654"
     CDS             complement(6081..6872)
                     /gene="nei"
                     /locus_tag="ACHKIK_003654"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO9084750.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNELTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERVLSPRFRNRQFAGLLLE
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6908..7642)
                     /gene="pxpA"
                     /locus_tag="ACHKIK_003655"
     CDS             complement(6908..7642)
                     /gene="pxpA"
                     /locus_tag="ACHKIK_003655"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO9084751.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEQQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7632..8564)
                     /gene="pxpC"
                     /locus_tag="ACHKIK_003656"
     CDS             complement(7632..8564)
                     /gene="pxpC"
                     /locus_tag="ACHKIK_003656"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO9084752.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGSGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8558..9214)
                     /gene="pxpB"
                     /locus_tag="ACHKIK_003657"
     CDS             complement(8558..9214)
                     /gene="pxpB"
                     /locus_tag="ACHKIK_003657"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO9084753.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9237..9980)
                     /gene="ybgI"
                     /locus_tag="ACHKIK_003658"
     CDS             complement(9237..9980)
                     /gene="ybgI"
                     /locus_tag="ACHKIK_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO9084754.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10251..11732
                     /gene="dtpD"
                     /locus_tag="ACHKIK_003659"
     CDS             10251..11732
                     /gene="dtpD"
                     /locus_tag="ACHKIK_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO9084755.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11774..13192)
                     /gene="phrB"
                     /locus_tag="ACHKIK_003660"
     CDS             complement(11774..13192)
                     /gene="phrB"
                     /locus_tag="ACHKIK_003660"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO9084756.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVKTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13189..13698)
                     /gene="ybgA"
                     /locus_tag="ACHKIK_003661"
     CDS             complement(13189..13698)
                     /gene="ybgA"
                     /locus_tag="ACHKIK_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO9084757.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13849..14397)
                     /locus_tag="ACHKIK_003662"
                     /pseudo
     CDS             complement(13849..14397)
                     /locus_tag="ACHKIK_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3463 a         3850 c         3503 g         3581 t
ORIGIN      
        1 ccgttatgtt taaaggacaa aaaacattgg ccgcactggc cgtatctctg ctgttcacag
       61 cacctgttta tgcggctgat gaaggatccg gtgaaattca ctttaaaggt gaagttattg
      121 aagcaccgtg tgaaatacat caggatgata ttgataaaga ggttgaactc ggtcaggtga
      181 ccaccagcca cattaatcag tcacatcaca gcgatgccgt tgctgtcgac ctgcgcttag
      241 tcaactgtga tctggaaaac tccagcaacg gttccggtgg caagatttct aaagttgcag
      301 tcacctttga tagctcagcg aaaaccaccg gcgcagatcc gattctcaac aacaccagca
      361 cgggtgaagc caccggcgtc ggcgtacgtt taatgaataa agatcaaagc aatatcgttt
      421 taggcaccgc tactccagat atcgacctgg ctccgacctc cagcgaacaa acgctgaatt
      481 tctttgcctg gatggaacaa attgatcagg ctacacctgt aacgccaggc gcagttacgg
      541 cgaatgcaac ctacgtgctg gattataaat aagtattact ttggcggcgc tatcgccgcc
      601 tttcataaaa tatttatcag gtatggacac agtgaatatt tatcgactct cttttgtatc
      661 ctgcctggtc gtggcgatgc cttgcgcatt ggcggtcgaa ttcaacctta atgttctcga
      721 taaatcaatg cgcgaccgca ttgatatttc attattaaag gaaaaaggag tcattgctcc
      781 cggtgagtat tttgttagcg ttgcggtaaa taataaccaa atcagtaacg ggcaaaagat
      841 taactggcac aaaaatgacg ataaaaccat tccgtgcatc aatgatttac tggtcgataa
      901 atttggctta aaacctgaag tccgtcagtc gttaccattg ataaatcagt gcgtcgattt
      961 tagctcccga cctgaaatgc tcttcaattt cgatcaagcc aatcagcaac taaatatcac
     1021 cattccgcaa gcctggctgg cgtggcactc agaaaactgg accccaccct ccacatggaa
     1081 agaaggtgtc gccggtgtcc tgatggatta caacttgttt gccagcagct accgcccaca
     1141 ggatggcagc agcagcacta atcttaacgc ctatggaaca gccggaatca acacgggggc
     1201 atggcgctta cgcagtgatt atcagttgaa tcagactgat agcgatgata accatgaaca
     1261 gtcaggcgaa atatcacgta cctatctttt tcgcccgtta ccgcaattag gttcaaaatt
     1321 aaccctcggc gaaacggatt ttagttccaa tattttcgac ggtttttctt ataccggcgc
     1381 ggcactggca agtgacgatc gaatgttgcc atgggaacta cgcggctacg ccccacaaat
     1441 tagcggtatt gcacagacca atgccacggt gacgatcagt caatcaggcc gcgtcattta
     1501 ccagaaaaaa gtcccaccag gcccatttat cattgacgac cttaatcagt ctgttcaggg
     1561 cacactggat gtcaaagtga cggaagaaga tggtcgggtg aacaatttcc aggtttcggc
     1621 agcatcgacg cccttcctga ctcgtcaggg acaggttcgc tataaactgg ccgcgggtca
     1681 gccacggccc tccatgtcac atcaaactga aaatgaaacc ttttttagca atgatgtttc
     1741 ctgggggatg ctgtcaaaca cctcgctgta cggcggcctg ctgctttctg gtgatgacta
     1801 ccattctgcc gcaataggta tcgggcaaaa tatgctgtgg cttggcgcgc tgtcgtttga
     1861 tgtcacgtgg gccagtagtc actttgatac tcagcaggac gagcggggct taagctaccg
     1921 ttttaattac agcaaacaag tggatgccac caacagcacg atttccctcg ccgcttatcg
     1981 tttctccgat cgtcattttc acagctacgc caactatctt gatcacaaat acaacgacag
     2041 cgatgcgcag gacgaaaaac agacgatcag cttatctgtg ggccaaccga ttaccccact
     2101 aaacctcaat ctttacgcca acctgctaca tcaaacctgg tggaatgcag acgcctccac
     2161 gaccgccaac atcacggcag gttttaatgt tgatattggt gactggagag atatctctat
     2221 ttcgacgtca ttcaatacaa cccattacga agataaagat cgcgacaacc agatttacct
     2281 gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc tatgacatgc aaaacagtag
     2341 ccacagcacc acacaccgca tgtcgtggaa cgatacgctg gatgaacgta atagctgggg
     2401 catgtctgcc ggactgcaat ccgaccgtcc ggacaatgga gcccaggtga gcggtaacta
     2461 tcagcatctg agttcagcgg gtgagtggga tatttctggt acctatgccg ccaatgatta
     2521 cagttccgtc agcagcagct ggagcggttc tttcaccgca acccaatatg gtgcagcgtt
     2581 tcatcgccgc agctccacca atgaaccacg cctgatggtc agcaccgatg gcgtggcaga
     2641 tattccggtt cagggcaatc tcgactacac caaccatttt ggcattgcgg tggtgccgtt
     2701 gatttccagt taccagcctt ccaccgtggc ggtgaacatg aatgacttac ccgacggcgt
     2761 aacagttgca gaaaacgtta tcaaagaaac gtggattgaa ggcgcgatag gttacaaatc
     2821 actggcttcc cgttccggta aagacgttaa cgtcatcatt cgcaacgcca gcggtcagtt
     2881 tcctcccctc ggagcggata tccgccagga tgacagcggc attagcgtgg ggatggttgg
     2941 cgaggaagga catgcctggt taagcggagt cgctgaaaat caaaagttta ccgtggtctg
     3001 gggtgatagc cagcattgct cgctccatct tcctgaacat atggaagaca ccgcaaatcg
     3061 cctgatttta ccttgtcatt aattaaggaa atgaaaatga catttatgaa aggactaccc
     3121 cttttgttgt tggttgccag tctgtgcagc cacgctgcac tacaacccga tcgcactcgt
     3181 attgtgttta acgctaatga taaagctacc agtctgcggg tggataatcg tagcgataaa
     3241 ctcccctacc tcgcttattc ctggctcgaa aatgaaaaag gggagaaaag cgatgatctt
     3301 ctggtcgccc tgccgccgat acaacgtctg gaaccgaaag caacaacgca ggtacggata
     3361 gtaaagcaag cctcgaccac aaaattaccg ggcgatcgcg aaacgctatt tttctacaat
     3421 atgcgtgaaa ttcctcccgc cccggaaaaa aacagcgacc atgccgttct gcaagtcgcc
     3481 attcagagtc gtatcaaggt gttctggcga ccggctgcat tacgcaagaa agcaggagaa
     3541 aaggtcgaac tgcagttaca ggtcagccag cagggcaacc aactgacact gaaaaatcct
     3601 accgcgtatt atctgaccat tgcctacctg ggacgaaatg aaaaaggcgt tctccccggc
     3661 ttcaaaaccg tgatggttgc gccttttagt acggtcaaca caaacatcgg aaattatagc
     3721 ggcagccagt tttatctcgg ttatatggat gattacggcg cgttacgcat gacgacgctc
     3781 aactacagcg gacaatgcca tttacaagcc gtggaggcga agaaatgagt gctggcaagg
     3841 gagtgttgct cgtcatctgt ctgttatttc tgccgttgaa gtctgcgctt gccctgaact
     3901 gctactttgg ttcatccggt ggctcggtag aaaaatcaga agcgattcaa ccttttgctg
     3961 taccaggcaa tgccaaactc ggcgataaga tttgggaatc tgacgatatt aaaattcccg
     4021 tctattgtga caacaatacc aacggcaatt ttgaaagtga gcatgtttat gcctgggtaa
     4081 acccctaccc gggggtgcag gatcgctatt atcaactcgg cgtaacttac aatggcgttg
     4141 attatgacgc caaccaggga aaaagccgca tcgacactaa ccagtgtatt gacagtaaga
     4201 acattgacat ttatacccct gagcagatta tcgcgatggg atggcagaac aaaatttgct
     4261 cgggtgatcc cgctaatatt cacatgtcac gtacctttct cgctcgcatg cggttatacg
     4321 tcaaaatacg agaaatgccg ccgcatgatt atcaaagtac gcttagcgac tatatcgtcg
     4381 tgcaatttga cggtgccggt agcgttaacg aagatcctac tgcccaaaac ctgaaatatc
     4441 atattaccgg tctggaaaac attcgcgtgc tggattgcag cgtcaatttt tccatttcac
     4501 cggaaacaca ggtgattgat tttggtaaat ttaatttgct ggatattcgt cgccacacaa
     4561 tgtcgaaaac attcagtatt aagacgacaa aatcacaaaa tgaccaatgc accgacggat
     4621 ttaaagtcag ttcctccttt tataccgaag aaacgttggt tgaagaagat aaagcattgt
     4681 tgattggtaa tggtctgaag ctacgtttgc tggatgaaaa cgcctcgcct tataccttca
     4741 ataaatatgc tgaatacgcc gatttcacca gcgacatgtt ggtctacgaa aaaacctata
     4801 cggctgaact ttcgtccatt gcaggtaccc ccatcgaggc tgggcccttc gatacagtgg
     4861 tgctttttaa aattaactat aactgatttg ccatgatgcc ctttgcagcc ggaaagggca
     4921 tctctttcag aaaattgctg attttcccct tttccttgat tgctgttatt ttttcctggc
     4981 ccggctttct ttatgctgta tcgatacacc aaagagaaaa taatgaggga gcgaaggatg
     5041 ccagttttgc agtggggaat gttatgtgtg ctatcacttc tcctttctat tggtttcctc
     5101 gcggtacatc tcccggcggc gctattactt gggccgatga tcgccgggat catcttcagt
     5161 atgcgcggaa ttactctgca acttccccgc tccgcttttc tcgccgcgca ggccattctt
     5221 ggctgcatga ttgcgcaaaa cctcactggt tcgattctca ccacgctggc cgccaactgg
     5281 ccgatcgtgc ttgctatttt actggtgacg ctgctttcca gcgccatcgt gggttggtta
     5341 ttggtgcgct atagctcact gcctggaaat accggtgcct ggggttcttc tccaggcggc
     5401 gcagcggcaa tggtcgctat ggcgcaagat tacggcgcag atattcgcct ggtggcgttt
     5461 atgcaatatc tgcgagtgct gttcgtcgcg ggggccgctg ttctggtcac ccgcatgatg
     5521 ctgggtgata acgctgaagc ggttaaccag caaatcgtct ggttcccgcc agtgagcatt
     5581 aatctcctgc ttacgatttt gctcgcagtc gttgccggta cggcgggatg catgctgcgt
     5641 cttccttccg gcacgatgct catcccgatg ctggcgggcg cagtgctcca gtctggtcag
     5701 ctcatcacca tcgaactccc ggaatggctg cttgcgatgg cgtatatggc aattggctgg
     5761 cggattggtc ttggtttcga taaacaaatc ttactgcggg cactacgccc gctaccgcaa
     5821 atcctgctgt cgatttttgc tctgttggct atttgtgcgg gtatggcgtg ggggctgacc
     5881 cggtttatgc atattgattt tatgactgcc tacctcgcca ccagccccgg cgggcttgat
     5941 accgtggcgg tcatcgccgc agggagcaat gccgatatgg cgcttattat ggcgatgcaa
     6001 accctgcgcc tgttcagtat tttgctgacg ggacctgcca ttgcacggtt tatttcaacc
     6061 tatgcgccga agcggtcggc ttagtgctgg cagccagggc accagtaaaa cgggcgagat
     6121 gacagcgtgg ttttctcaat gatgctgcca caacgttcgc acggttcgcc atctcgatga
     6181 aaaaccttaa agcgaaacag cgccccatga tgcttattct catccacctg cccccgcgta
     6241 gcgtaagaaa atcgaggaat ctccagtaac gcgtgtgcga gtgcatccag ttgcgccgca
     6301 ttgagatctt tcgctttatg atttccagtc aacccaacct gccagaggat ctccacccgc
     6361 aaataattgc caagtcctgc cagaaacgcc tgctcgagca gtaatcctgc aaactgacgg
     6421 ttacgaaagc gcggcgacaa tacccgttct ttcaccacct ccggcgtcag attcggatcc
     6481 agcacatcgg gaccaacgcg ttgtaaaaac ggatgcgtgg tcagttgttc cggggtcaac
     6541 atctcaatat ccgaggcgct ataaagcaga atggttttgt cagccgtttg cagttttacc
     6601 cgcaatactc gcgtggtctg cggctcttcg ccggtatcaa ccacgcgcca gacgccgtaa
     6661 agctgattat ggctgtagag cgttaattcg ttggaaaaat gagttaacaa cgccttacca
     6721 cgcgtttcca catgggtaac gtgttgaccg ataagttgtg attgataagt ttttaactgc
     6781 gggaaggcaa accagacatc agttagtggt ttgcctttga tcgccgcctc caggttatcc
     6841 gctgcacggc ggatctccgg gccttcaggc atggtgatat ccttttttca ttctttatta
     6901 agcggggtta tgctgcgaca acaatcccct ttttggcaaa tgtagctcgt aatcggcggg
     6961 cgaacgccag agcgtgctcg ccgtcgccat gcaggcagac cgtttgcgcc gcgaccgttg
     7021 cccattcgcc ggtgatactt ttgactctgc cgtgttgcac catctccagc gtttgcgcca
     7081 gcgcctgttg ttcgttttca atcaacgcgc ctggctgact tcgcggcacc agcgagccgt
     7141 cagcctgata accgcgatcg gcaaacactt cctcgcgcgt tgtcaggcca tattgcttgc
     7201 ctgcacgaat cagctcgctt cctgccagcc caacgagaat caatgccgga tcgcaagcgt
     7261 ataccgctct ggcgatggcg tctgccagtt gtgcttcttt cgccgcctgg ttgtacaaca
     7321 tgccgtgcgg tttgacatga cgcattacgc cgccttgcgc acgggcaatc gttgccagcg
     7381 cgccaatttg atacagcgtc tgggcgtaaa cggtttctgg cggcaactgc atggcgctgc
     7441 gaccaaaatt ttccctgtcg ggaaaactcg ggtgagcgcc aattgcaaca ccatttttta
     7501 ttgcttcacg cacgcaagcc tgcatggttt gcgcatcgcc tgcatgaaat ccacaggcaa
     7561 tattggcaga ggaaaccagc gttaatagct ctgcgtcgct ggcgcagcct tcgcccagat
     7621 cggcgttcag gtcaattttc attgtgcagc cgccacgcta attgttcaaa ataacgttgc
     7681 tgatcttgcc gcgctttcag tgcctcttcc agtgaacact ggacaaaatg aatcggctga
     7741 ccgagcggaa tttgcgcaag atggtacata tcagcctcaa tgatgcaggc aatacgcggg
     7801 taaccaccgg tggtctgtgc gtcgttcatc aacacaatcg gctgcccgtt atgtggcacc
     7861 tgcaccacgc ccggtaacaa accgtgagat aacagttcgc gatcggtggt gcgttttaaa
     7921 atttgcccct gtaagcgata gcccatgcgg ttactttgcg agctaagctg ccagggcgaa
     7981 cgccagaatg catcctgcga ggcgcgatcg aactcatgat attccggccc cggcaaggcg
     8041 cgaatgcggt tgccccacag cagctgttta acgccctgcg cttccataaa atcacgcttc
     8101 gctttgccaa tcgggagtcg gtcaccatcc ttcagtaaac ggccttccag cccgccactc
     8161 cccactttga gatcggtgct gcatgacccc attaccggcg gaacatcaat accacccgcg
     8221 accgccagat aactgcgcat cccgtgctgc gggcgtttaa gcgttaaacg ctggcctgct
     8281 ttcatcggca atcgccagcc ggtccagacg gcattatcat ccagccgcgc ttcgcaaccg
     8341 gcacccgtca gagcaaacca cccatcagtt tcgaactcaa cagttaactg accgagcgtg
     8401 atctccagcg cgggggcatt ggcgtcatta cccaccagta ggttagcaat gcgtaacgcg
     8461 ggcatatcca gtgcgccgca gtggctgata cccgactggc gaaaaccgtg acgaccgcca
     8521 tcctgcacag tggtatacat gcccgcacga ataatcttca gcatactccc tccttctgcg
     8581 gtacaaagcg cacgctgtct cccggacgta ataagatggg ttcgtcacgc gccggatcaa
     8641 acaggctgag tgaggtatga ccaatcaact gccagccacc cggcgttgcc agcggataaa
     8701 caccagtctg cggcccgccg atcccgacag aacctgccgg aacgagtaag cgcggttcag
     8761 cgcgccgtgg cgtgtgtagt tgttccggca aactcccgag atacgggaag cccggttgaa
     8821 aacctaaaaa ccagaccacg tattccacgg aggagtgcaa ttcaacaacc tgtttttcgc
     8881 tcaacccgca atgcgccgcg accaccgcca aatccggtcc gcctgcacca ccgtaaacca
     8941 ccggaatttc aataaagcga gactcaggtt ccagggcctc gctctcctcc caccagcgtt
     9001 gcaaacgctc tatggcatcc agcgccagcg actcaggatt acgcaaaatc accgtaatat
     9061 tgttcatgcc gggaatggct tcaaccacat tcggcatatc caccagacgc tgcgccagtc
     9121 gccagatccg tttctggcta gccagcgtca ccggcggttc cagttccagc actaccgccg
     9181 tttcacctat cagataacaa cgcgctcgtt gcactttcga tccctctgat tattcgttat
     9241 gcaggattag gaatatcaat aaaggtcaca tcaagatcgg tattttcatt cagccactcg
     9301 ctcaatgcgc gaataccacc acgttcagtg gcatggtgac ctgcagcata aaaatgcaat
     9361 ccctgctcgc gggctgaatg aatggtctgt tcagaaactt cgccagtaat aaaagcatcc
     9421 acgccaaaac gcgcggcgct atcgataaaa ctttgcccgc cgcccgtgca ccaggcgacg
     9481 cgctggacca cctcaggtcc ggtatcgcca caccataacg gcttacgtcc cagacgcgct
     9541 tcaatccagg aggctagttc cagtcccggc acaggcatgg tcagctctcc ccacggcacc
     9601 aacggctcaa tttcgcccat gacagtgatc cccagtaacg ccgccagctg tgcgttattg
     9661 cccagctcag gatgtgcgtc aagcggcaaa tgccagccat acaggttgat atcattcgcc
     9721 agcaacgttt ttaaacggtt acgcttcatt ccgcgaatga ccggagactc tcctttccag
     9781 aagtagccat gatgcacaat gaccgcatcc gcgcccagac gcactgcctc atcgagcaaa
     9841 gcctggctgg cggtgacacc ggtaacaatt ttttgcaccg tctctttgcc ttccacctgc
     9901 aaaccattcg gcgcgtaatc actaatcgcc gcgctgttca gtttttcgtt aatcagttgt
     9961 tccagttcgg tgtttttcat cttcgcgcct tccgtattct gatagctaca ccgttaacat
    10021 agcgttacct cgcctgttcg tcgatagcca atcttttgac atattaagaa acaaacccga
    10081 cgccaaaaga cgcaaattgt ttatatcatc acgcttcagc taatgtatat ttatgcaatc
    10141 agacagcata ttgcataaat aatcagatct gtatcaaagt tttaacttta aggaaacaca
    10201 agcacgccgc gcaactatta cgtgacgttg ccgtcaattg tggaattatt atgaataaac
    10261 acgcatcaca accgcgcgct atttactatg tcgttgcgct gcaaatctgg gaatatttta
    10321 gcttttacgg catgcgtgcc ctgctgattc tctatctcac caatcaacta aaatacaacg
    10381 atactcacgc ctacgagtta tttagcgcct actgttcgct ggtgtatgtc acgccaatcc
    10441 tcggtggctt tttggcggat aaagttctcg gcaatcgcat ggcggtgatg ctgggggcgt
    10501 tgttgatggc gatcggtcat gtggtgctgg gtgccagtga gatccatccg tcattcctct
    10561 atctgtccct ggcgattatc gtttgtggct atggtctgtt taaatccaat gtcagttgcc
    10621 tgctcggcga gctgtatgag ccaaccgatc cgcgtcgtga tggcggtttc tcgctgatgt
    10681 atgcagcggg taacgtgggg tctattatcg cacctatcgc ctgtggttac gcccaggaag
    10741 agtacagctg ggcgatgggc tttggcctgg cggcggtggg tatgatcgcg ggtctggtca
    10801 ttttcttatg tggcaatcgt catttcacac atacccgcgg cgttaataaa aaagtactgc
    10861 gtgcaacaaa ctttctcctg cctaactggg gatggctgct ggttctgctg gtggcaacgc
    10921 ctgcgctgat taccgtactg ttctggaaag agtggtcggt atacgcctta attgtcgcga
    10981 ctattattgg cctgggtgta ctggcaaaaa tttatcgcaa agcagaaaac cagaaacagc
    11041 gtaaggagct ggggctgatt gtgacgctca ccttcttcag tatgttgttc tgggccttcg
    11101 cacaacaggg cggcagctcg attagccttt atatcgaccg cttcgttaac cgcgatatgt
    11161 ttggttatac cgttccgact gctatgttcc agtcgattaa tgccttcgca gttatgctgt
    11221 gcggtgtgtt cctggcgtgg gtggttaaag aaagcgtcgc aggtaatcgt accgtgcgca
    11281 tctggggaaa atttgctctt ggccttggcc tgatgagcgc cggattctgc attctgacct
    11341 taagcgcccg ctggtccgca atgtatggtc actcttctct gccactgatg gtattaggcc
    11401 tggcggtgat gggctttgcg gaactgttta tcgacccggt tgccatgtcg caaattacgc
    11461 gcattgaaat tccgggcgtg accggcgtat taaccggcat ctacatgctg ctttctggcg
    11521 cgatcgcgaa ctatctggcg ggcgtgattg ccgatcagac atcgcaggct tcgtttgatg
    11581 cttccggggc gatcaactac tccatcaatg catatattga agtatttgat caaattacct
    11641 ggggcgcact ggcgtgtgta ggactggtac tgatgatttg gctgtatcag gcgctgaaat
    11701 tcagaaaccg cgcgctggcg ctggagtcat aataggccag caccatacct gataaaacgc
    11761 tcgctgcgtt ttatcaggct cctttcctcg cttcttcgta cgctgccagc gttcgcagcc
    11821 ttgcctcttt gtgatcaact atcggttgcg gataatccag catcacacct gctttctgcg
    11881 cccacttcca cggctcatgc accgctttcc ctggtacatc gcgcagctcc ggtaaccatc
    11941 ggcggataaa ctcaccttcg cgatcaaatt tctcgccctg ggttgtcggg ttgaaaatac
    12001 gaaaatacgg cgctgcatca gtacccgtag acgccgccca ctgccagcca ccgttattgg
    12061 ctgccaaatc accatcaatc agctgcgaca tgaaatatcg ctcgccttcg cgccagtcga
    12121 tcaacaaatc tttcaccaga aaactggctg aaatcatccg tagccggtta tgcatccagc
    12181 cagtgctgtt aagctgacgc atggcggcat cgacaatcgg gtatcccgtt ttgccttcct
    12241 gccaggcctg taaatgtgcg ggattgctct gccactgcac acgatccgtc caggcaataa
    12301 acggacaatg tttacacaac gaggggtaat acgtcatcag atgacggtag aactcgcgcc
    12361 agatcagctc actaagccag acactaccgg ccccaccgtc cagcgcctgc ggctgttccg
    12421 ccaacaagcg atgcaaacac tggcgaggcg ataacccgcc cgttgccagg ctggcggaca
    12481 aacgactggt gccttccact gccggaaaat ctcgttgttg ctcatattct ccggcaccgt
    12541 tctggcaaaa ctggcgtaat tgcgcaatcg ccgctttttc ttccaccggg aaatgcgcag
    12601 tatcgaaaga ctgacgagga taattcagcg taatggatgg cgagggctct atcgatccgc
    12661 tactacgaac ttttggcgca gcgacgcact ccggcatccc ttcccgcagc cgtttcagcc
    12721 aggcattctt aaaaggcgta aagactttgt acatctcgtg attaccggtc ttcaccgcgc
    12781 caggcggcag gatcacgctg tcatcaaatc cttcacacac cacgttacgc agcgctcttt
    12841 caacttccac atcccgcgcc cgctcattca cttcatactg atagttataa aacaggtggg
    12901 taacgctgtt ttccgcgcac acctgtttaa ctatttcgac actggcgaca aagtcatcca
    12961 cttcacggaa caataaagga ataccttttt ccgcaagcgc tatttgtagc ccattcagtt
    13021 gagcattgat aagttcagcc tgacgcggcg acatgttatg cgtcgcccac tggcgtggtg
    13081 tagcgatata caacgccagc acgcgtgcag acgaattgcg gcaggcggca gccagtgcga
    13141 gattatcgtg cagacgtaaa tcctggcgaa accagaccag atgggtagtc ataaaactcc
    13201 tgaatggcgc aagttaatca cacgcggcca aagttcgaaa taacgctgcc ctgaaagcca
    13261 ggcgtcagga taaagcgcca tatagtgttt gatacgcatc agcggcgcaa gaagtggttg
    13321 ctcgccacgg cgataactgt cgataagcgc cgccaactgc tggcgttctg tggaatcaat
    13381 atgcgggcga aaataaccct gaacgtgcat caaaacgttg gtgtgatcgc gcgggttggc
    13441 gggatgagaa agcaaaacaa ttacgcgttg gtagtactgg ttgtaaaaat catccagatt
    13501 ttgccactgg tgaatatcag ccacgaacgg gccaagtttg cggtactccg gctgagaatg
    13561 cgccagaaag acgagtttat agcggctgtg ataatcgaga agcgccccgc gcgttaagcc
    13621 atgttctttc agtcgatgta actcatgaag ggcgaagata cggattaggg tgctgtcatc
    13681 aaatcgttgc tgattcatta ctgctcctga acggtttaca ggagtaagtg tagacgttga
    13741 gaaatgatat ttgtgaagtt aatcctgttg tagcaggaca aatttacgaa agcctgctcc
    13801 ccgcaaggac tgacgtcagg tagtttctaa ccatagctgc cctttctatt caaacgtgaa
    13861 atcaataagc tcatcaggga catcgcaaac aatatgaaga cgggtttctt ctctgccgtg
    13921 actcttttca ctaattgcgt aactgtcatg cttgggatta tttaattctt tcagcggaaa
    13981 tttttcctcg aaggctttat ttagccgccc ctggtttcct tttacagcga ataaataatc
    14041 acctccctgt ttttgtatct tctctgcaat atctttctgg caacccatcg catcggttgt
    14101 gatgattttt cctttaatat ccagcatgtt aagaagttct gggatagctg tgatctcatt
    14161 ggatttctta tccgtcttga tctgcccgat gaccagactg tgcattgttg agaacgcact
    14221 aatgacatga atcgctcccc tgcggcgact cttgtcataa gagtgccgga gcgtttttcc
    14281 atcaattgca atgacgtctt tatcatttga tgaatggcaa tcacgcatcc agttaataaa
    14341 gcactcgtga aattttgcag gactgataca ggatacaact ctggcaatag tatcgtg
//
LOCUS       DAZGJW010000120        14302 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZGJW010000120 DAZGJW010000000
VERSION     DAZGJW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.120"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            226..1797
                     /gene="garD"
                     /locus_tag="ACHKIK_003663"
     CDS             226..1797
                     /gene="garD"
                     /locus_tag="ACHKIK_003663"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO9084758.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWLDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDIASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1946..2281
                     /gene="prlF"
                     /locus_tag="ACHKIK_003664"
     CDS             1946..2281
                     /gene="prlF"
                     /locus_tag="ACHKIK_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO9084759.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2281..2745
                     /gene="yhaV"
                     /locus_tag="ACHKIK_003665"
     CDS             2281..2745
                     /gene="yhaV"
                     /locus_tag="ACHKIK_003665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO9084760.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(2800..3609)
                     /gene="agaR"
                     /locus_tag="ACHKIK_003666"
     CDS             complement(2800..3609)
                     /gene="agaR"
                     /locus_tag="ACHKIK_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO9084761.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVVERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3858..5138
                     /gene="kbaZ"
                     /locus_tag="ACHKIK_003667"
     CDS             3858..5138
                     /gene="kbaZ"
                     /locus_tag="ACHKIK_003667"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO9084762.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCHDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5161..5634
                     /gene="agaV"
                     /locus_tag="ACHKIK_003668"
     CDS             5161..5634
                     /gene="agaV"
                     /locus_tag="ACHKIK_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO9084763.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5645..6424
                     /gene="agaW"
                     /locus_tag="ACHKIK_003669"
     CDS             5645..6424
                     /gene="agaW"
                     /locus_tag="ACHKIK_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO9084764.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6414..7292
                     /gene="agaE"
                     /locus_tag="ACHKIK_003670"
     CDS             6414..7292
                     /gene="agaE"
                     /locus_tag="ACHKIK_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO9084765.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFILLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7310..7744
                     /gene="agaF"
                     /locus_tag="ACHKIK_003671"
     CDS             7310..7744
                     /gene="agaF"
                     /locus_tag="ACHKIK_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO9084766.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLALQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7741..8874
                     /gene="nagA"
                     /locus_tag="ACHKIK_003672"
     CDS             7741..8874
                     /gene="nagA"
                     /locus_tag="ACHKIK_003672"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO9084767.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            9225..10379
                     /locus_tag="ACHKIK_003673"
     CDS             9225..10379
                     /locus_tag="ACHKIK_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO9084768.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10392..11252
                     /gene="kbaY"
                     /locus_tag="ACHKIK_003674"
     CDS             10392..11252
                     /gene="kbaY"
                     /locus_tag="ACHKIK_003674"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO9084769.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11419..11895
                     /gene="agaB"
                     /locus_tag="ACHKIK_003675"
     CDS             11419..11895
                     /gene="agaB"
                     /locus_tag="ACHKIK_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO9084770.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11934..12737
                     /gene="agaC"
                     /locus_tag="ACHKIK_003676"
     CDS             11934..12737
                     /gene="agaC"
                     /locus_tag="ACHKIK_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO9084771.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12727..13518
                     /gene="agaD"
                     /locus_tag="ACHKIK_003677"
     CDS             12727..13518
                     /gene="agaD"
                     /locus_tag="ACHKIK_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO9084772.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13519..14274
                     /locus_tag="ACHKIK_003678"
     CDS             13519..14274
                     /locus_tag="ACHKIK_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO9084773.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQLLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT         3458 a         3623 c         3723 g         3498 t
ORIGIN      
        1 atgctattta aatattattt tgagcatatg cacataaggt tgcgcgctaa agcacagatt
       61 tgcgctttac cttgccgggc ggcactgcaa tccctgaaat gattgacatt gatcacattt
      121 ctgcgtttaa actcctgaca ttcttatttc acccaatgaa gtcatttatt tttaaatgag
      181 accaggtcct cattttaata acccctggct ggagaatata gtacaatggc caacatcgaa
      241 atcagacaag aaacgccaac tgcgttttat ataaaagttc acgacacaga taatgtggca
      301 attattgtta atgataatgg cctgaaagca ggaacgcgtt ttccggatgg actggaatta
      361 attgaacata ttccccaggg gcataaagtc gcattgctgg acattccggc taatggtgaa
      421 attattcgtt atggtgaagt gattggttac gccgtgcgtg caatcccacg cggaagctgg
      481 ctcgacgaat caatggttgt actaccggaa gcgccgccgt tacacacgct gccactggca
      541 accaaagtcc cggccccctt accgccgctg gaaggataca cctttgaggg ctatcgcaat
      601 gccgatggca gcgtgggcac caaaaacctg ctcggcatca ccaccagcgt ccactgtgtg
      661 gcaggcgtgg tggactatgt agtaaaaatc attgaacgcg atctgctacc gaaatacccg
      721 aacgtcgatg gcgtggtggg gctgaatcat ttgtacggct gtggcgtggc gattaacgca
      781 ccggcggcag ttgtgcctat tcgtaccatc cacaatattt cgctgaaccc taactttggc
      841 ggcgaagtaa tggtgattgg tctgggttgt gaaaagttgc agcctgagcg cctgctggtc
      901 ggcacggatg atgtgcaagc tattccagta gaaagcgcca gcattgtcag tttgcaggat
      961 gaaaagcatg tcggttttca gtccatggtc gaggatattt tgcaggtcgc cgaacgccat
     1021 ctacaaaaac tgaatcaacg tcagcgagaa acctgtccgg cttcagaact ggttgtcggc
     1081 atgcagtgcg gtggcagcga tgccttttct ggcgtaacgg caaacccggc ggttggctat
     1141 gcgtctgatc tactggtgcg ctgcggcgca acggtgatgt tctcagaagt cacggaagtg
     1201 cgtgacgcga tccatctgct gacaccacgc gcagtgaacg aagaggtcgg caaacggctg
     1261 ctggaggaga tggagtggta cgataactat ctcaatatgg gaaaaaccga ccgcagcgcc
     1321 aacccttcgc cgggcaacaa gaaaggcggt ctggcaaacg tggtagagaa ggcactcggc
     1381 tccattgcta aatcgggtaa aagcgcaatt gttgaagtgc tgtcgcccgg tcaacgcccg
     1441 actaaacgcg gattaattta cgccgcgacg ccagccagcg attttgtctg tggcacgcaa
     1501 caggtggctt cgggtatcac cgtgcaagtg tttacgaccg gtcgtggtac gccgtacggc
     1561 ctgatggcgg tacccgtcat taaaatggcg acccgcaccg agctggcgaa ccgctggttt
     1621 gatttaatgg atattaacgc gggcactatc gccaccggcg aagaaaccat tgaagaggtg
     1681 ggctggaagt tgttccactt tattctcgac atcgccagcg ggaagaagaa aaccttctcg
     1741 gatcaatggg ggctgcataa ccagctggca gtgtttaacc cggcaccggt gacctgattt
     1801 cttttggaac gtccctcgca aaacatggcc ttagtgccat gtttttattg tttaaagccc
     1861 ccacgtccat taataatgca tttgcattac cttagttcaa gcttataatt tgagcagaaa
     1921 acaggctgta aaaggacagt gaatcatgcc cgctaatgct cgctctcacg ctgtactgac
     1981 cactgaatca aaggtcacga tacgcggaca aacaactatc cccgcaccag tgcgtgaggc
     2041 cttaaaactg aagccaggcc aggacagcat tcattacgaa attctgcctg gtgggcaagt
     2101 ctttatgtgc cgactgggag atgaacagga ggatcatact atgaatgcat ttttgcgttt
     2161 tctggatgca gatatccaga acaacccgca aaaaactcgt ccattcaaca ttcaacaagg
     2221 aaagaaactt gtcgctggca tggacgtcaa cattgatgat gagattggcg acgacgaata
     2281 atggattttc cacaaagggt taatggttgg gcgctatatg ctcatccctg ttttcaggaa
     2341 acctacgacg ctttagttgc cgaagtcgag acattaaagg gaaaagatcc tgaaaattat
     2401 cagagaaaag ccgccacaaa gttattggcg gtagtccata aagtgattga ggagcatatc
     2461 acggtcaatc catcatcacc ggcattccgt catggcaagt cgttaggctc tgggaaaaat
     2521 aaagactggt cacgggtaaa atttggtgct ggtcgttatc gtctcttctt tcgttatagt
     2581 gaaaaagaga aagtcatcat tctgggatgg atgaacgatg aaaacactct gcgcacctac
     2641 ggtaaaaaaa cagatgccta taccgtattc agcaaaatgt taaaaagagg acatcctcct
     2701 gccgactggg aaaccctcac ccgagaaaca gaagaaaccc attgatggtg ttcacaatgc
     2761 ccgccgaacg tattcgtgtt gaccagcggg caccctttct cactccccga ccagaatcac
     2821 ttcaacccca gcctttcgca gtccttccag actatccgca ggaatgcctt catcaacaat
     2881 gatcatgtcg atacgttgag tatcaatgat cttatgtaaa ctggaacgat tgaatttact
     2941 ggaatcggtg accacgatga tccgttccac aacttcgcac atccgacggt ttaaacgggc
     3001 ttcatcttca ttatgtgtgc tgacgccgcg ctccagatcg atcgcatcta caccaagaaa
     3061 cagcatatcg aagtggtaat tttgcagcga ttgctcagcc tgatcgccgt aaaaagattg
     3121 cgactgacgg cgcaaatgcc cgccggtcat cagcagctca acgccttccg cttccagcaa
     3181 cgcattagcc acgttcatac cgttggtcat cgcaattacg tcagtgtgct tacgcatcag
     3241 acgagcaatc tcaaaagtgg tagtcccgga atcgaggatc acccgatgac caggctgaat
     3301 caactcaacg gcagctttcg caacgctgcg tttcatcgcg gtgttcagcg cgcttttatc
     3361 ttccactgat ggctcgactg acggcgtcgt gctatcgcag atcaacgcgc caccataggc
     3421 acgcacagcg atcccctgct tttccagaaa cgccagatca ttgcggatcg tcacagtaga
     3481 tacgccatac aatgccgaca gatcgttaac ctgcacactc ccttgctgtc gcagacgctg
     3541 aatgatctgt tctcgtcgct cgctggtgcc agtcactcgc ttctcacctg aagcgtcggt
     3601 attactcata gtaagtcctt tcgtaaaact ttcgtttcat ttcgttttgc ctattaacgc
     3661 ctttctatta agcaaatgca agcccacctt gcccattgac gcaagctact ctcgtttcag
     3721 tgactttcat tatgtttctt ttgtgaatca gatcagaaaa ctattatctt tcgttttatt
     3781 tttatctcac catgacgcag tatcaactga aacaaaacga aagattaata tcgcagtaat
     3841 ctgaactgga gaggaaagtg aaacatctga cagaaatggt gagacagcac aaagcgggca
     3901 aaacaaatgg aatttatgcc gtttgttccg cacatccgct ggtgctggag gctgcaatcc
     3961 gctacgccag tgcaaaccaa acgccgttac tgattgaagc aacctccaat caggtagacc
     4021 agttcggcgg ttataccgga atgacgcccg ccgattttcg cggctttgtt tgtcagctcg
     4081 ccgactcgtt gaatttcccg caggatgcgt tgattctggg tggtgaccat ctggggccaa
     4141 accgctggca aaacctgccg gccgctcagg caatggccaa tgccgatgat ttgattaaaa
     4201 gctacgttgc ggcaggattc aaaaaaatcc accttgattg cagcatgtcc tgtcatgacg
     4261 atccgattcc cttaactgat gacatcgtgg ctgaacgcgc cgcccgtctg gcgaaagtgg
     4321 cggaagaaac ctgtcttgaa cactttggcg aagccgatct ggagtatgtc attggtaccg
     4381 aagtgccggt acctggcggc gcgcatgaaa ccttaagcga gctggcggtc accacgccgg
     4441 atgccgcccg cgccacgctg gaagcccatc gtcacgcctt tgaaaagcaa ggtttgaatg
     4501 ccatctggcc acgcatcatt gccctggtgg ttcaacccgg cgtcgaattc gatcacacca
     4561 acgttattga ttatcagccc gccaaagcga gcgccttaag ccagatggtc gaaaactacg
     4621 aaacgctgat tttcgaagcg cactctaccg attatcaaac gccgcaatcg ctgcgccagc
     4681 tggtgattga ccactttgcc attctgaaag ttggcccagc gctgaccttc gccctgcgtg
     4741 aagctctgtt ctctctggcg gcgattgaag aagaactggt gccagcgaaa gcctgttctg
     4801 gtctgcgtca ggtgctggaa gacgtgatgc tcgaccgccc ggaatactgg caaagccact
     4861 accacggtga cggcaacgcg cgtcgtctgg cgcgtggtta tagctactcg gatcgcgtgc
     4921 gctattactg gccggacagc cagattgatg acgctttcgc tcatctggtt cgtaatctgg
     4981 cggattcacc aattccgcta ccgctcatca gccagtatct gccgctgcaa tacgtgaaag
     5041 ttcgctccgg cgagctgcaa ccgacgccac gggaactcat tatcaaccat attcaggaca
     5101 tcctggcgca gtaccacaca gcctgtgaag gccaataagc aaaacaaaga ggaacacgct
     5161 atgccaaata ttgttttaag ccggattgat gaacgcttga ttcacggtca ggtcggcgtt
     5221 cagtgggtcg gatttgcggg ggcaaatctg gtgctggtag ccaacgatga ggttgccgaa
     5281 gatccggtac aacaaaacct gatggaaatg gtactggcag aagggatcgc cgtacgtttc
     5341 tggacgctgc aaaaagttat cgacaacatt catcgcgccg ccgatcgaca gaaaatcctg
     5401 ctggtttgta aaacacccgc cgatttcctg acgctggtga aaggtggcgt tccggtgaat
     5461 cgcattaacg ttggcaatat gcactacgcc aatggcaaac aacaaatcgc caaaacggtt
     5521 tctgtggatg cgggcgatat cgcagcattt aacgacctga aagccgctgg ggtggaatgc
     5581 ttcgttcagg gcgtcccgac agagcctgct gtagacctct ttaaattact ttgagggatt
     5641 catcatggaa atcagcctgt tgcaggcatt tgcgttgggc attatcgcct ttatcgctgg
     5701 cctggatatg tttaacggcc taacacatat gcaccgcccg gtggtcctcg gcccgttggt
     5761 cgggctggta cttggcgatc tgcataccgg aattttaacc ggcggtacgc tggaactggt
     5821 gtggatgggg ctggccccgc tggcgggcgc acagccgcct aacgtgatta tcggtactat
     5881 cgtcggcacg gcgtttgcca ttactactgg cgtgaaaccc gatgtcgcgg tcggtgtcgc
     5941 cgtacctttc gctgtcgcag tacagatggg gattaccttc ctgttctcgg tgatgtctgg
     6001 cgtgatgtct cgctgcgacc ggatggcgga gaacgccgat actcgcggca ttgaacgcgt
     6061 gaactatctg gcactgctgg cactcggcac tttctatttt ctctgcgctt tcctgcctat
     6121 ctacttcggc gcggaacatg caaaaaccat cattgatgtc ctgccgcaac gattaatcga
     6181 cggcctcggc gtcgcgggcg gcatcatgcc agcaatcggt tttgccgtgc tgctgaaaat
     6241 catgatgaaa aacgtctaca tcccctactt catcctgggc tttgttgccg cagcctggct
     6301 caagttaccg gtgctggcta ttgctgccgc tgcgctggcg atggcgctga tcgacctgct
     6361 gcgtaaatct cctgaaccga ctcaacctgc ggcacagaaa gaggaatttg aagatggcat
     6421 ctaatcaaac caccctgccg aacgtcagtg aaaacgaaga aacattgctg actggcgtca
     6481 atgaaaacgt gtatgaagat cagagcattg gcgcggagct gacgaaaaaa gatatcaacc
     6541 gcgtcgcctg gcgttccatg ctgttacaag cgtcttttaa ctacgaacgt atgcaggctt
     6601 ctggctggtt gtacggtctg ctgcctgctc tgaaaaagat ccacactaat aaacgcgacc
     6661 tggcgcgcgc catgaagggg catatgggtt tcttcaatac ccatccgttt ctggtgacat
     6721 ttgttatcgg cattatcctt gcgatggagc gttctaagca ggacgttaac agtattcaga
     6781 gcaccaaaat tgccgtcggt gcgccgctcg gcgggattgg cgatgcaatg ttctggctaa
     6841 cgctactgcc gatttgtggc gggattggag ccagtctggc attgcaaggc tccattcttg
     6901 gcgcagtcgt ctttattctg ctgttcaacg tggtacacct ggggctgcgt tttggtctgg
     6961 cgcattatgc ttaccgcatg ggcgtggcgg cgattccact cattaaagcg aataccaaaa
     7021 aagtcggcca tgctgcgtct atcgttggga tgacggtaat cggtgcgctg gtggcaacct
     7081 atgttcgttt aagcaccacg ctggaaatca cagcgggcga cgcagtggtt aagttacagg
     7141 ctgatgttat cgacaaactg atgcctgcct tcttaccgtt ggtctacacc ctgaccatgt
     7201 tttggttggt acgccgcggc tggagtccgc tgcgtctgat tgcagttacc gtggttctcg
     7261 gcatcgtcgg taaattctgc catttccttt aaatacaatg aggtattaga tgttaagtat
     7321 tattttgaca gggcatggcg ggtttgccag cggcatggaa aaagcaatga agcaaattct
     7381 cggtgagcaa tcgcagttta tcgccatcga ttttccggaa acctcaagca ccgcgctgct
     7441 tacatcgcag cttgaagagg ccatcgccca actggattgt gaagacggca ttgtttttct
     7501 gacggatttg ctgggcggca cgccgtttcg cgtagcttca acgctggcgc tgcaaaaacc
     7561 gggctgtgaa gtgatcaccg gcaccaattt gcaactgcta ctggagatgg tgctggagcg
     7621 cgaagggtta agcggtgaag aattccgtgt acaggcgctg gagtgtggac atcgtgggct
     7681 gaccagtctg gtggacgagt taggccgctg ccatgaagaa tgtccggtcg aggaagggat
     7741 atgacacacg ttctgcgcgc cagaaggctg ctgactgaag agggatggct cgatgaccat
     7801 cagttgcgta ttgctgacgg tgtcatcgca gcaatcgaac cgattccagt gggcgtgact
     7861 gaacgcgatg cggaactgct ctgccccgct tacatcgaca cccatgtaca cggtggtgcg
     7921 ggcgttgatg taatggatga cgcgccggat gtactcgaca agctggcaat gcacaaggca
     7981 cgcgaaggtg tcggcagttg gttgccgact accgtaaccg cgccgcttaa caccattcat
     8041 gcggcgctga aacgtattgc tcaacgttgc caacgcggcg gacctggtgc gcaagtgctg
     8101 gggagttatc tcgaaggacc gtacttcacg ccgcagaata aaggcgcgca tccgccggaa
     8161 ttgtttcgcg agcttgaaat tgccgagctg gatcaattga ttgccgtttc tcagcacacc
     8221 ttacgcgtgg tagcgctggc accggaaaaa gagggggcat tgcaggccat ccgccatctt
     8281 aaacagcaaa atgtacgagt gatgctgggg catagcgcgg cgacctggca acaaactcgc
     8341 gccgcgtttg atgctggtgc cgacggcctg gtgcattgct ataacgggat gacaggttta
     8401 catcaccgcg aaccgggaat ggttggcgcg ggattaacgg acaagcgcgc ctggctggaa
     8461 ctgatagccg atggtcatca tgtgcatccg gcggcgatgt cgctgtgttg ttgctgtgca
     8521 aaagagagaa tcgtgctgat caccgacgcg atgcaggcag ccgggatgcc ggatggtcgc
     8581 tatacgttat gtggcgaaga agtgcagatg cacggtggcg ttgtccgtac cgcgtccggt
     8641 gggctggcgg gcagtacgct gtctgttgat gcggcagtgc gcaacatggt cgagttgacg
     8701 ggcgtaacgc ctgcggaagc cattcatatg gcatcgctgc atccggcgcg aatgctgggt
     8761 gttgatggtg ttctgggatc gcttaaaccg ggcaaacgcg ccagcatcgt tgcgctggat
     8821 agcgggctgc atgtgcaaca aatctggatt cagagtcaat tagcttcgtt ttgatatttt
     8881 gctcctttat tgggccttca cttcccccgt aaggcctttc tttttctttc gttttgatct
     8941 gtgcagcggt gtcggatgcg acgctaacgc gtcttatgcg acctacggta cgccgcatcc
     9001 agcaatcggt gaacatagcg acgtcattac gcaattcttt aatctcccct tgtcttttct
     9061 ttcaatgatc acagatttcg ttttatttcc tttctctcca ttgaactttc ggtttctttt
     9121 ctatagattt taatcaacga aagacatcac caagtgaaat gaaacgaaag gcaagtgaaa
     9181 gcgacaacgc ccgacgtcaa gttcatcaga ctaaggattg agttatgcca gaaaattaca
     9241 cccctgctgc cgccgcaacc ggtacatgga ctgaagaaga gatccgccat cagcctcgcg
     9301 catggatccg ttcactcacc aacatcgacg cgctacgttc tgcgctcaat aacttccttg
     9361 aaccgttact gcgcaaagag aatctgcgga tcatcctgac cggagccgga acctcggcat
     9421 ttatcggtga catcatcgcg ccgtggctcg ccagccatac cggtaaaaac ttcagcgccg
     9481 taccgaccac cgatctggtc accaatccga tggactacct gaacccagct catccgctgc
     9541 tgttgatctc cttcggtcga tccggcaaca gcccggaaag cgtcgctgcc gtggaactgg
     9601 caaatcaatt tgtaccagaa tgctatcacc tgccgatcac ctgcaacgaa gcgggcgctc
     9661 tttaccaaaa cgcgatcaac agcgataacg cgtttgccct gctgatgccc gcagaaacgc
     9721 acgatcgcgg ctttgcgatg accagcagca ttaccaccat gatggccagc tgcctcgcgg
     9781 ttttcgcacc tgagacgatc aacagccaaa ccttccgcga cgtggcggat cgttgccagg
     9841 cgatcctgac ctcactgggc gatttcagcg aaggtgtgtt tggttacgca ccgtggaaac
     9901 ggatcgttta tctcggcagc ggtggcttac agggcgcagc acgcgagtcg gcgctgaaag
     9961 tgctggaact gactgcgggt aaactggcgg ccttctatga ttccccgacc ggattccgtc
    10021 atggcccgaa atcactggtc gataacgaaa cgctggtggt ggtatttgtc tcaagccacc
    10081 cttacacccg tcagtatgat cttgatctgc tggctgaact ccgccgtgac aaccaggcaa
    10141 tgcgcgtaat cgccatcgcc gcggaaagca ccgacatcgt cgctgccggt ccacatatta
    10201 tcctgccgcc gtcacgtcac tttatcgacg ttgagcaggc attttgcttc ctgatgtacg
    10261 cccagacgtt tgcactgatg cagtcgctgc acatgggcaa tacgccggat accccatcag
    10321 ccagcggcac cgttaaccgc gtggtgcaag gcgtaatcat tcatccgtgg caggcataag
    10381 aggatcgcat tatgagcatt atctccacta aatatctgtt acaggacgcc caggccaatg
    10441 gctacgcggt gcctgctttt aacattcata acgccgagac gatccaggcg atcctcgaag
    10501 tgtgcagtga aatgcgatcg ccggtgatcc tcgccggaac gccggggacc tttaaacata
    10561 tcgcgctgga agagatctac gccctgtgca gcgcctattc cacaacctac aacatgccac
    10621 tggcgctgca tctcgaccac cacgaatcgc tggatgatat tcgccgtaaa gtccacgcag
    10681 gtgtgcgcag tgcaatgatc gacggcagcc acttcccgtt tgccgagaac gtgaagctgg
    10741 tgaaatcggt tgttgacttc tgccactcac aggattgcag cgtggaagca gaactgggcc
    10801 gcctgggcgg tgttgaagat gacatgagcg ttgacgccga aagtgcattc ctgaccgatc
    10861 cacaagaagc taaacgcttt gtcgaactga ctggcgtcga cagcctggcg gtagcgattg
    10921 gtacggcgca cggcttatac agcaaaacgc cgaagattga tttccagcgg ctggcggaaa
    10981 ttcgtgaagt ggtggatgtt cctctggtgc tacatggtgc cagcgatgtt ccggatgaat
    11041 ttgtccgtcg cactattgaa cttggcgtca caaaagtgaa cgttgccaca gaattaaaaa
    11101 tagccttcgc tggcgcggtt aaagcctggt ttgcggaaaa tccgcagggt aatgatcctc
    11161 gttattatat gcgcgtcgga atggatgcga tgaaagaagt tgtcagaaat aaaattaatg
    11221 tctgtggttc agcgaatcga atttcagcat aatcattagt tttttatgat ttatcccaat
    11281 gtacttcccg attaatatcg gggagtgcct taatggaaaa ggagatgact aaaccttaat
    11341 aaatacatca gtacaatatc gcaacaataa tatattaaaa aaaattatat tattcaactt
    11401 tatggtgagg attacacaat gagcagtcca aatattctct taacccgtat tgataaccgt
    11461 ctggttcacg gtcaggttgg cgtgacctgg acatccacca tcggtgcgaa tctgctggta
    11521 gtcgtggatg atgttgtcgc taacgatgat attcaacaga aattaatggg tattaccgca
    11581 gaaacctacg gctttggcat tcgtttcttt actatcgaaa aaaccattaa cgtcatcggc
    11641 aaagctgcac cgcatcagaa gatcttcctg atttgccgta cgccacaaac ggtacgtaaa
    11701 ttggtagaag gtggcattga cctgaaagat atcaacgtcg gcaatatgca tttctcggaa
    11761 gggaaaaagc aaatcagcag taaagtttat gtcgatgacc aggatctcgc ggacttacgt
    11821 tttattaaac aacgtggcgt gaatgttttc attcaggacg tccctggcga tcaaaaagaa
    11881 caaatccctg actaaatcta aaatcgcctt aatattggtt tgaggtaata aaaatgcatg
    11941 aaataaccct acttcaggga ttatccctgg cggcgttagt tttcgttctg gggattgatt
    12001 tttggctgga agccttattt ttattccgcc cgataatcgt ttgtacccta actggcgcta
    12061 ttctcggtga tattcagact ggcttaatta ccggtggtct gacagagttg gctttcgccg
    12121 gattaacccc tgcaggtggt gttcagccgc ccaacccgat tatggcgggt ctgatgacca
    12181 ccgtcattgc atggtctacg ggcgttgatg ccaaaacagc aattggtctt ggcctgccgt
    12241 ttagtttgtt aatgcagtac gtcattctgt tcttctattc cgctttctca ttatttatga
    12301 ccaaagccga taaatgcgcg aaagaggcgg atacggcagc gttttcccgg cttaactgga
    12361 caacgatgct catcgtcgct tcagcgtatg cggtgattgc tttcctctgt acttacctgg
    12421 cacaaggggc gatgcaggcg ctggtgaaag cgatgcccgc ctggttgacc cacggctttg
    12481 aagtggctgg cggtattctg cctgccgttg gttttggctt gctgctgcgc gtaatgttca
    12541 aagcgcaata tatcccttac ctgatcgccg gtttcctgtt tgtttgctac atccaggtca
    12601 gcaacctgtt gccggttgcc gtactgggcg caggctttgc ggtgtatgag tttttcaatg
    12661 cgaaatcccg gcagcaagcg caaccgcagc ccgttgccag taaaaatgaa gaagaggact
    12721 acagcaatgg gatctgaaat cagtaaaaaa gatatcaccc gtctgggctt tcgttcgtcg
    12781 ctgctgcaag cgagctttaa ctacgaaagg atgcaggcgg gcggttttac ctgggcgatg
    12841 ttgccgatcc tgaaaaagat ttataaggac gacaaaccgg gcttaagcgc ggcgatgaaa
    12901 gataacctcg aatttattaa tacccacccg aatctggtcg gattcctgat ggggttatta
    12961 atttcgatgg aagaaaaagg agaaaaccgc gacaccatta aaggcctcaa agtggcactg
    13021 tttggcccaa tcgccgggat tggcgatgcg attttctggt ttactttgtt gccgattatg
    13081 gcgggaattt gctcatcatt tgccagccag ggaaacctgc tggggccgat tctatttttc
    13141 gccgtttacc tgcttatctt tttcctgcgc gtcggctgga cccacgtcgg ttattcagtc
    13201 ggcgtgaagg cgatcgataa agtgcgagag aactcgcaga tgattgctcg ctcggcaacc
    13261 atcctcggga tcacggtaat cggcgggctg atcgcttcgt atgtgcatat taacgtggtg
    13321 acatcgtttg ccatcgacag tacccacagc gtcgcgctgc aacaggattt cttcgataaa
    13381 gtcttcccga acattttacc gatggcctac accctgctga tgtattactt cctgcgggtg
    13441 aaaaaagcgc atccggtgct gttaatcggc gtgacttttg tgctctctat tgtttgttcc
    13501 gcattcggca ttttgtaaat ggaacgaggc actgcttctg gcggtgcctc tttacttaag
    13561 gaatttcatc ctgtgcaaac ccttcagcaa gttgaaaact atacggcgtt aagtgaacga
    13621 gccagcgaat atttattggc tgtgatccgt agcaaaccgg atgccgtgat ttgcctggcg
    13681 accggagcca cgccattact gacgtatcat tatctggtag aaaaaatcca ccagcagcag
    13741 gttgatatca gccagctcac cttcgtgaag ctcgacgaat gggtggatct gccattaacg
    13801 atgcctggca cctgcgaaac tttcctacaa cagcatatcg tgcagctgct ggggctgcgt
    13861 gaagaccagc tcatcagctt tcgctccgaa gagataaatg agacagagtg cgaacgggta
    13921 acgaacctga ttgcgcgcaa aggcggtctg gatttatgcg ttctaggatt ggggaaaaac
    13981 ggtcatcttg ggctgaacga accgggagaa agcctacaac cggcctgcca tatcagtcaa
    14041 cttgatgcca gaacacagca acatgagatg ttaaaaacca cgggtcgccc cgtgactcgt
    14101 gggatcacct taggcctgaa ggatattctc aatgcccgcg aggttttgtt attggtgact
    14161 ggcgaaggaa agcaggatgc gacagaacgt tttctcacgg ctaaagtctc caccgctatc
    14221 ccggcttcgt ttttatggct gcacagtaac tttatttgtt taattaatac ctaaccagat
    14281 gaaaatttgc tcaaaatttt ta
//
LOCUS       DAZGJW010000121        14090 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZGJW010000121 DAZGJW010000000
VERSION     DAZGJW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.121"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(55..630)
                     /locus_tag="ACHKIK_003679"
     CDS             complement(55..630)
                     /locus_tag="ACHKIK_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO9084774.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(699..1277)
                     /gene="terD"
                     /locus_tag="ACHKIK_003680"
     CDS             complement(699..1277)
                     /gene="terD"
                     /locus_tag="ACHKIK_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFO9084775.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(1326..2366)
                     /gene="terC"
                     /locus_tag="ACHKIK_003681"
     CDS             complement(1326..2366)
                     /gene="terC"
                     /locus_tag="ACHKIK_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFO9084776.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(2389..2844)
                     /locus_tag="ACHKIK_003682"
     CDS             complement(2389..2844)
                     /locus_tag="ACHKIK_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO9084777.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(2867..4024)
                     /locus_tag="ACHKIK_003683"
     CDS             complement(2867..4024)
                     /locus_tag="ACHKIK_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO9084778.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(4024..4605)
                     /locus_tag="ACHKIK_003684"
     CDS             complement(4024..4605)
                     /locus_tag="ACHKIK_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO9084779.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            4928..5986
                     /locus_tag="ACHKIK_003685"
     CDS             4928..5986
                     /locus_tag="ACHKIK_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFO9084780.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            5996..7138
                     /locus_tag="ACHKIK_003686"
     CDS             5996..7138
                     /locus_tag="ACHKIK_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFO9084781.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            7131..7904
                     /locus_tag="ACHKIK_003687"
     CDS             7131..7904
                     /locus_tag="ACHKIK_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084782.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            7906..8985
                     /locus_tag="ACHKIK_003688"
     CDS             7906..8985
                     /locus_tag="ACHKIK_003688"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFO9084783.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            8985..9941
                     /locus_tag="ACHKIK_003689"
     CDS             8985..9941
                     /locus_tag="ACHKIK_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFO9084784.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            9952..11160
                     /locus_tag="ACHKIK_003690"
     CDS             9952..11160
                     /locus_tag="ACHKIK_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFO9084785.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            11178..11645
                     /gene="terW"
                     /locus_tag="ACHKIK_003691"
     CDS             11178..11645
                     /gene="terW"
                     /locus_tag="ACHKIK_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFO9084786.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            11906..12235
                     /locus_tag="ACHKIK_003692"
     CDS             11906..12235
                     /locus_tag="ACHKIK_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9084787.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            12222..12602
                     /gene="tnpB"
                     /locus_tag="ACHKIK_003693"
     CDS             12222..12602
                     /gene="tnpB"
                     /locus_tag="ACHKIK_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9084788.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQEEPEDAQSNAITQ"
     gene            12645..13475
                     /locus_tag="ACHKIK_003694"
                     /pseudo
     CDS             12645..13475
                     /locus_tag="ACHKIK_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(13506..>14090)
                     /locus_tag="ACHKIK_003695"
     CDS             complement(13506..>14090)
                     /locus_tag="ACHKIK_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO9084789.1"
                     /translation="EREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYA
                     IEDEIRGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDA
                     LCYYSDDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTNK"
BASE COUNT         3436 a         3731 c         3596 g         3327 t
ORIGIN      
        1 cggatcgata cccaaaaaaa accccggcat tgccggggtc agactcagcg tcttttagac
       61 attgacgccg tgctgggaag caagcgctga cagaccaccg gcaaaaccct ggccaacagc
      121 tttaaacttc cactcagtac catggcgata cagttcaccg aagaccattg cggtttcggt
      181 tgaggcgtct tcagacagat cgaaacgggc aatttccgtc ccgttgtcgt tgttgtaaac
      241 gcgcatgaag ctgttgctca ccatgccgaa gttttgttta cgcgcttctg catcatagat
      301 ggtaacggca aataccagtt ttttgatgtc tgctgagact ttggtcagat cgattttgac
      361 ctgctcatcg tcgccgtcgc cttcaccggt acggttgtcg ccctggtgct ctactgcgcc
      421 atcagggctg gttttattat tgaagaaaat gaaatgggca tctgacagta ctttaccgtc
      481 ttcacctact gcgaatacgg aagcgtccag atcaaaaccc tgaccatcgg ttacacgggc
      541 atcccagccc aggccaacca tagcgacatt catggttggt gcttctttgg tcagagatac
      601 gttgccgcct tttacgagag aaactgccat ttttagctcc tgcaaacagt tgaatgaggc
      661 tgaattaaca gccccagata tgaaaagtta cttttcgatc aggacgcgtt aatgccgtac
      721 tgagcacata cagatgccag accaccagca taaccctggc ctactgcgcg gaatttccac
      781 tcaccattgt ggcgatacag ctcgccgaac agcatggcag tctcagtgga cgcatcttcg
      841 gtcagatcgt agcgagcgac ttcagtctgg ttatcgtcat taaccagacg aataaacgca
      901 ccggatacct gaccaaagct ctggcgacga gcctgagcat cgtggatggt cacaacgaag
      961 atgatcttgt caacttcaga cgggacggcg tccagtttaa ttttcagcga ttcatcatca
     1021 ccatcgccct caccggtgcg gttatcgccg gtgtgcgtta cggaaccgtc ggatgacgtc
     1081 aggttgttat agaagatgaa atctgaatcg ccgcgcactt tgccgtttga ggccagcagg
     1141 aatgctgaag catccaggtc aaagtcctga ccgtctgttg aacgcgcatc ccagccaagg
     1201 cccaccagga catttttcat tgacggagct gctttactca gggagacgtt cccgcctttg
     1261 gaaagagaaa cactcataaa ataacctctt cgattagtaa ttgttcaggt taacacttaa
     1321 ggggattaac tccccttttc ctcagattca ggtttgcccg ggaacatgac gcttgcgaga
     1381 atgcccagcg ccagtacacc cagtacaaca tacaggctgg ttgttgccgc gatgctgtaa
     1441 ccatgatgcc agatgtgatc ggtcgcattc aggccgagtt ttgccgcgat gaagaacagc
     1501 agcacgataa cggccttctc cagatgaacc aggtactgtt tcagtgcctc aaggacaaaa
     1561 tacagagtac gcaggcccag gatagcaaac atcatggcac tatagacgat aagcggttca
     1621 cgactgacgg caatgattgc cggtaccgag tcaaacgcga acattacgtc cgagagttca
     1681 accacagcca cacacaggaa cagcggggtc gcatacagcg ccgctttttt accacggcca
     1741 atggtgacat cgctgttttc tggtttcgcc agttcagcat ccacttcctt ctggttaagt
     1801 aggaaggcat gccctctgag cttcggccag atagggaaga agcgtttaac catacggtaa
     1861 gccagatgct gggagtaatc ctcaatttca tcatcgtcat caccgctttt aagcatcatg
     1921 accgccgtcc aggcaacgat aatagcgaag acaacttcaa cgtacggccc cagactcagc
     1981 aggctcgtac cgatggcgac aaagatgccc ctgaagacga tggcaccaat gatcccccag
     2041 tagagaacac ggtggcgata acgatccgga acggcgaacc aggagaagat tgccatcatg
     2101 acgaacaggt tatcgaccga cagcactttc tccagcgcat aacccgtgac aaacagactg
     2161 gcaacctcag caccgtggtg gatatagagg aaaccggcaa atgccatcgc aaccacaacc
     2221 cagaatacgg accagagcgc agcactcttc agcgagattg gcttgtcatc acggtgcata
     2281 aacaggtcaa taaagatggc accgactgaa agcgcaataa aaacaatgac cgtttcagtc
     2341 gggaagccga tgtgtgtgga aaccatattt tacccttagg gatgtggatc agaggccaaa
     2401 ttcagccggg tcgaagccca acgcgatagc gatctcgcgg gaggccagtt tctcgtcctg
     2461 atcgaagtta ccgtcacttt tcgcaacggc aataccaaca cgtaaggcca gctgagcggc
     2521 ctcaggctga tctttcagcg ccaggatgta tttcatggtt tcgcccttgc caatttcaac
     2581 atcgaaatcg aagcttgaaa ccagtttatt gaagaactca atcacctcat tggtatcgaa
     2641 gaccttcagc tcttctgaag agcgcagaaa gcccatcatt ttctgctttt cttccgaact
     2701 tacgccgtca ctcgatacgg caatacgggc acatacagca acggtgccct gcatgaattt
     2761 tttgtttttg aaacggccaa cctggcgggt cagttcgtca cggcctgagt taatggcacc
     2821 tttaactttg tcgaaaaagc tcatacctgt ttcctttgtt gctgtgttat ttagaaccgg
     2881 cgctccagcg aaatccccag ccaaatgccc ggtccatttc gtcctggcct ttgaagtact
     2941 ggttaattcg ctcgacttta attgcgccgt tttcgtttac gagtctggca atggcgcaca
     3001 atgtgcgacg gttctcacct tcggtcaggc gggtctcaat gggcggttga tccggcacat
     3061 gaatggtgac tacaccatca gtcttgtccc agctgggaac accttcataa ataaaggcgt
     3121 aaatcagcac ttcgcggata tgcttccatt cacgtccatt gatatgcagc cactcgccgt
     3181 ctgatacatc acccgtccgg tcatcgccct tgagctgaac atacggttca tcgcgataat
     3241 caccgaatgc attaccgagc gcctgaatga ccgatttata cccgtcctga agctcaacaa
     3301 aggcccccag atccaggtcg attcctttgg agccgaacat acctgcaaag cctgatttgc
     3361 tactgccccg gtgccagttc aggttgatcc ggatttcacc gaagttatcc cgtttggtca
     3421 ggctgattgc aggcttctct tttgtcagcg ataccttgct caggctgatt tttgtctcaa
     3481 ctggcggagg ggttacaacc ggaggagttg gggctgcggg agccggttcg tcggcaatat
     3541 ttacgccgaa atgctctgcc agcggtttaa gtccaccgtt gaacccctgc gcaacgaagc
     3601 ggaatttcca gtcattatta cgacggtaaa attcccccag aattaaggct gcttcctgac
     3661 ggccgctcag ttcgacactg ccgctaacca ggccggtagc cccttgctcc acgtcgatgg
     3721 aaagattacg aagaccggac accgtctgcc caccgtcaca ggtcacggtg aacgcaattt
     3781 tctggacatc aggtttcagc cggttgagcg cgacagtaaa ggttgagtac tggccttcac
     3841 tgaccaggct gacggtacca tcatcgttac gtggctgacc ataaaacacc atgtccgcat
     3901 caccctgcac cttcccgtcg gcatacaggc gaaacgccga agcatccacc tggccgcccg
     3961 aggtgatccg gacccgcaac tcctgggctg gaacgggggc attgcccccc ggcgtcaggt
     4021 tcattagcga ataaccgctg aaacgatatc ggagtgcata tcgtcaattg tgcggccgcg
     4081 acaggcatgg ccaagggcgg taaagtccca gttgccgtta ttacggcgca gcgaagaaat
     4141 aacgatcccg gtatgtgagc cctgctcagt cagcttgtag cgagccagct ctttgccggt
     4201 ttgatcgaca acgcggcaga aagcgttttc gacgtcattg aacgactgac cacggaagct
     4261 gtttaccgta aacgccaggt attcaacgtt tgcaggcagg cgggagaggt tgaccttaat
     4321 cacttcatcg tcaccgtcac cctcaccggt caggttatcg ccactgtgca caacagcgcc
     4381 gcaggtggat ttcagtttgc ggaaccagat ggtgtcaatt gttttacctg tgctgtccat
     4441 taatacacag ccagcatcga gatcgattga gtcgttgcct ccaaacaatc ctccaagaag
     4501 accttttttc ttaaccggat cccaaccgag accgaaatga agctggctta atgcagatga
     4561 ttctttgctg agtgataccg tctggttctt aactaatgaa accatgtttt attccttaaa
     4621 atggtaatga ccgagacaat caaaggtata tttagtaact gacgaagaaa atcatatcat
     4681 gatgaagttc tctgacaagg gaaatttttg tggttttctg taaaaacaca aaaagaaatc
     4741 cttttcaggg tgttagtttt tctattttta atttaaatat caatgtgtta taaggaatcc
     4801 aaaaaacact gacgcactcc ataaccacag gagttattaa tcatattatg attgacatgt
     4861 agcttagaca ctcatagact cacttctcaa tcataaattt gcatggcgtt ccggataatt
     4921 aaaaaaaatg aacaagaaaa tttggtttat ggaaggttta tcctcccagc gagatattat
     4981 tcagggggta aaatcatttg cacaaaaaaa taattttgcc attaccgttt ttgcctccca
     5041 ccgtaacgaa agaaatgaaa tcctttccgt tgccgattat tctttgactg aacctgaaga
     5101 tcctcaaaaa cgtcttcagt ttatccagga aaccattcag acctacggca tccaccatat
     5161 tcatactggc cgtaacagcc agtggtttga agaacaccgt tcagccattg aaccgaccgg
     5221 tgccaccctt actaccggtg caacgggcgt cgactggtta actctggctg acgaaaaagt
     5281 tacttttgct cagtttatgg agcaaaaggg tctcccggtc gtaccatcct ggcgggtgaa
     5341 tacgctggca gaattaaaga cacacctcgc ggccccgccg ttcactgaca gcccggtatg
     5401 cgtgaagccg gtgacgggta tctatggcat gggattctgg cgctttgatg acagtgcttc
     5461 gcctatggcc gtctttaatc atcccgaaca tcgtctggtc agtccgcaac agtatattgc
     5521 agcagcatca gctgctgagt cgtttaaacc ccttgttttg atgccgtacc tgccaggccc
     5581 ggaattttcc gtcgatatcc tcgcggataa gggcgaaata ctcgcagccg tgggacgccg
     5641 taaggaaggg gctatccagt atctggtaaa cgaaggaagc gcctgggaac tggcgtgtga
     5701 ctgcgcccgt gttatgaagg ccgacgggct ggtgaatgtt cagacgcgaa acgatgtgaa
     5761 tggcaacccg gtgctgcttg aaaccaacat gcgtccgtca gggggggtgg gttataccct
     5821 tcacagcggt gtgaaccttc ctgggttatt tgctgccttt aagctcggtc tgatgtctga
     5881 agatatggta cgccagagcg ctaaaaacac cttttctccg gttgcggtga gatccattac
     5941 ggatgtaatt gcatacccgg aatcactctc taaccttctg aattaaggcg tatttatgtc
     6001 tgatatcctc gaagatatga tttatcgccg taccctctca tgcggtacga ttcaggtaac
     6061 ccgtgaccag ggtgaagttt cgctcgatga cctgtttgac atcgctgaaa gacgtaaccc
     6121 gaaacgcgcc tttctgtttg tcagcaaagt actcggcagg cacattcccg tgccaccctc
     6181 agtcatgcga caggcctaca gacagcttgc cagccagttc ccctcgacac tgacaggacc
     6241 cgtactgttt atcggtatgg ctgaaaccgc cgttgggctt ggggccggtg tatttgatga
     6301 agtgcgccac cagcatcgtg aatctgtcta tctgacctct acccgtcacc cggttgatgg
     6361 cacgttgctt tgcgagttca aggaagaaca cagccacgcc accgatcatc tgatctatct
     6421 gccagatgat gaagagaaaa gacgtcgtgt taccaacgca cgaacgctgg ttttgattga
     6481 tgacgaggca accaccggta atacctttat taacctgctt tctgccctgc gtaataccgg
     6541 caagcttcaa catattgaac aggttatcgc cgtaacgctg accgactgga gtggcaatgc
     6601 cctgtcggag cgcaccccct tgcccgtcac ttcggtttcg cttgtaagcg gtcagtgggg
     6661 atggacccca ttacctgatg cacctgtccc ggacatgccg aaagtcaacg taacttcacg
     6721 aggagaatgg gacatccagg ggaaacagtc atggggccga ctggggatgc tcgcacctgc
     6781 ggccgatctc ggccatgagg tctccgtcca caagggggaa cgtattctgg ttctcgggac
     6841 cggggaattc gtctgggagc cgttcctgct tgctgaacgg ctcgaagctg ccggagcaca
     6901 ggcattttat ggatcgacca cccgctcccc tgtcgccgtt ggttatgcca ttgagtccgc
     6961 catttccttt acggataact acgggctggg catccccaat tttgtctata acgtcgccca
     7021 ccagcagttt gaccgcattc ttgtgtgtac tgagacaccc gcagaaagta ttgacacgca
     7081 gcttcttaaa gcgctggctg aggttgcgcc cgtcgtggag attgttacct atgaataaac
     7141 ccgttgttct gagcgacctt gacgacacgc tctttcagac ccgtcgtaaa atggtggacg
     7201 agctggccct ggagccattc cgtaccggtg ccgttgatcg caccctgaat ccacgaagct
     7261 ttatgacgga agaacagtcc atgttggtgg actggctcct ggagcaggct gaactcatac
     7321 ccgttaccgc acggggaact gaagaaatca gccgcgtacg gatccctttc cactcctggg
     7381 caatcacaac gcacggggcc gtcattctca cgcctgaagg caaacccgac gaggagtgga
     7441 aggcccatat gctcggccag ctggctccct accaggaaaa gctgacatcg atgcagcgtc
     7501 tgatcactga aatgatggac gcaaaaggaa tcaatgcctg ggcaaggctg aacttcgaat
     7561 acggtgaaac ggcggtttat atggtgatga aacaccgcga cagcacccgc cttgacgagc
     7621 tcaatgccat tgcagatgag atagaaacgg tgtttccgac cgagggcttc tacatccacc
     7681 gcaacagtaa taacgtggcc tggcttccca ccccggttga gaaagggctg gcagtcagct
     7741 ggctgcttga aaaacttcgg gctgaacgag gagttttccc cgtaattggt ttgggcgaca
     7801 gcctgagcga tcatcgtttt atgaaactgt gcagctggtt tggcatcccg cgtcagagtc
     7861 agtttgcaga tgccatttca cagcgaattt ttggagaaaa ttaaaatgca gccacatgaa
     7921 acatttaccg gctcatacca gcccggtgac gtggaatttc tgctaaagcc ggttgtcatt
     7981 gagatgacgc cggttgagaa aaaagaagaa ctgattcagt ccgggaagaa acattattcg
     8041 gacatgctca gccaggagcc agcgcctaca caatggcatc ttgatttgtt tcaccgggcg
     8101 ctggatcggg gcgcagagag gcttgcaaaa gaagtcacac agctcgctat tgctctggcc
     8161 gaacgcttcg gtgatgagcc cattgtactg gccagtcttg tcagggctgg cgttccgctc
     8221 ggcgttatgc tgcaccaggc cctgcgtgac atggggaaaa cctcatggca ttacggtatc
     8281 agcattatcc gggatcgtgg aattgacggt gcagccctcg acgtcattga agagcggcat
     8341 ggtaccagcg gtattgtctt tgttgacgga tggacaggta aaggcgcaat taccggagag
     8401 cttgtacgcg cactgaaaga tcgcccgggc tatcctgagc agccgcgact ggttgtcctg
     8461 gccgatccct gtggctgctc ctggcttgca gccagtgatg atgactggct cattcctttt
     8521 ggcatcatgg gggcgccggt atcaggcctg atctcccggt cagtatggtc ctctgaagga
     8581 ttacacgggt gcatggtttg cgagcatctc agtgaattcg aatgcagccg gatgcttgcc
     8641 gataccgtcg ctcatttccg taagaagtta acaccgtcat ccctcgctcc cctgagctgg
     8701 aatacggaat cagcccgggt cttatggcag acaagtcgcg acgttatcgc gttcctggcc
     8761 gatgaattta aagtggacag cgtcaatcgt attaaacccg gtattgctga agcaacccgg
     8821 gctgtattac gtcgggtacc ggaccatgta tttgtgcgtt ctattgacga cccggacgtt
     8881 gccttgcttg tagggcttgc tcgtgaaaag ggaatagttg ttacagaaat ggggggaacc
     8941 ctcggccagt atcgggctgt aaccattatc aagaaggtac tctgatgaaa aatcgtcttt
     9001 ctccctggaa tctgggagcc acgctataca tgcctgcaac acgggaagat attgctgatg
     9061 ccgtcctgca cgggaaaatc ccgggcttgc gctctctggt gatttgcctg gaggatgctg
     9121 tcagtgaagc agacatcccc gtcgccctca aaaatctgga gcacctgctg cacgagctca
     9181 gtaacagcat gagtagcctg ggtaaaaatg actggcctct ggtgttcatt cgcccccggc
     9241 atgccgaaat gggcagatgg ttaaaagcgc attatgatct ttccgctgtc gacgggtttg
     9301 tgctgcctaa atttaccctc agttcgttag ctgaatggtg ggatatcatg gtcgggactc
     9361 acctgtgcat gatgccaacg ctggaaacag aagatgtctt tgacgtggtt cagatgcgcg
     9421 agctggctac ccgtctggag gaacatccct gccacgaccg cattattgcg cttcgaatcg
     9481 gcggcaacga tcttatgaac gttgtgtcgc ttcgccgtcc ccgggacctg acgctgtatg
     9541 acagcccgat gggctacgtc atcaaaatgc tcgtttcggt cttcggcccg cgtgattttg
     9601 ccctgaccgc gcctgtatgt gagcatattg acgatcatgc cgttatggcc agagagctgg
     9661 ctcttgatat ggcacacggg cttgttggga aaacggccat tcacccgggt cagatagagg
     9721 tcattcaaaa cgcgctgatg gtcactcagg gtgagcattc tgacgctctg cggatcctga
     9781 actctaccca ggccgtgttt aagtcgcagg gagcaatgtg tgaacccgcc acacatcgcc
     9841 gctgggcggc tggcattctg gacagggctc gtttttatgg gttacagaac gagcaaagcg
     9901 ctgatggaat cagattactt accgtgaccc agcatcatta aggaaatcat aatggggttc
     9961 aggttcagaa agtcgatcaa cattattcct ggcgttcgcc tcaacctgag taacggtgca
    10021 ccgagcctga gtgtcgggcc gagaggtgct tccgtttctt ttggtagccg ggggacctat
    10081 gccaatctgg gcttgcccgg taccgggctg agttaccgta cccggcttga ccgggccgcg
    10141 cgttccagag gtgaaaaccg gacggcaacc gacccggggc tcagacaggc gcttgagcag
    10201 aaagccgctg aactcatgtc agcggtaacc gcaatccgta atattcacga gctgacgccg
    10261 gatccaaaaa caggcatcag ctgggcagag ctggaagcag tatacctgca taacagaacg
    10321 tcgccttttc aggttccggc accggtgcgt ccagaaaagc cagactacct tgtattgccg
    10381 gaaaagcctg ccgagagcga aggcattagt tttttgggta aatggtttga atcggaatca
    10441 gctaaagctg agcgccacgc cgaaaatctt cgccggtggc agcaggagct gattgatgtg
    10501 gagcgtgaga atacccttcg acagcaccgg taccagcaac aacggacggc ctgggccgaa
    10561 cagtatgcaa actggaagtt tgaagctgaa gaacatgaaa aacggctcgc cacggctcag
    10621 gcagatgccc ggcagcagtt ccggacagac gccgcgtttt tcgaatcata cctggcgggt
    10681 gtgctggcag aaactgaatg gccgcgtgaa acgcttgttg catttgaagt aaagccggag
    10741 ctatcagcag tcctgctgga cgttgattta gctgagattg aagatttccc tgataagatt
    10801 tacggcgtta atgcccgggg aacggagctg acggaaaaag ccatgacgca aaaagccgta
    10861 cgcgaaaact atgcccacca cgtccatggc tgcttgttcc gcctggtcgg tatcgtttta
    10921 catacgctac ctttcgacaa cgtgattgtg tcaggcttta cgcaacgggt cagtaagcgg
    10981 accggctatc tggaggatga gtatatcctg tcctgcaaat gcactcgcag ccagatgtcg
    11041 tcagtaaatt ttgcaggcat aaaacacatt gatccggttg aagcgttagg cgatcaaccg
    11101 gttattcgaa agatgagcag taccttcatt tttcagccta ttgaaccact aaccctttaa
    11161 gcaggctaaa tcttacgatg caattaaata ccagacaggc ccggattttt aagcttgcca
    11221 acttactggg caccggcaag cccgtttccg ctgccgacat tataaccagt ctggaatgct
    11281 ctgagccgac attaacccgg gccctcaaag agctacgcga gtcatattct gcggagatca
    11341 aatacagcaa ggcaggtcat tcctatcatc tcgttaatcc gggccagttg gataaaaaga
    11401 ccctccgccg gatgaatgaa gcgctcgcac agaatgctga actgaaaacc ggtgagtcca
    11461 cagggaaggt cgtactcgat aaggataaaa aaacagccgt gtcattatcg ctacgcatgc
    11521 ggattctaag aaaaattgac cgtctggctg cgctgagtga ctcgacccga agtgaagcgg
    11581 tagagaagct ggctctgcac tccgttgatg aactgataaa agaatacagc gctaaaaagt
    11641 cctgacgcta aatctcagac cggggtgaaa actccggttt tgtcatattt cacctctgta
    11701 ttggcgtgat gcaaaatggc atctgaatcg atcatggttt ggcatccatt tccgatcaca
    11761 gtttggcatc acaatcgatc acgatttggc atgcttccgc tcattgattg gcatcctggt
    11821 gtcgttacgg ggaagtcatc ttcacaggga tcttcagtca gttagcatta ccgacatcca
    11881 ttcaccaact taccggagat aatcaatgtc aaaaccccga tggacgcttg atcagaaaaa
    11941 acatcacgtt gccgcatggc gcgccagcgg ccttacccgt gaacagtact gtgaacttta
    12001 cgacattccg tttaaatccc tgcgccagtg gccgcaggat gtcgcaaaag ccgaaaaacg
    12061 agccagagca ccagaaatta ttcctgtttc agtatctggc tcttccggaa tgactgatgg
    12121 cagaccattg tcggatgagc ctgtcactct cttcctgccc ggtggtatcc ggatgtgctg
    12181 ccagccttct cagttaaccg atgttttccg ggctcttcgc catgctgacg cctgataacg
    12241 tattcctggt cgtaaaaccc gtcgatatgc gccggggtat cgacacactg acacagtacg
    12301 tccagaacga acttaacgct gcatggcatg acggtgctgc ttttgtcttc accaataaag
    12361 tccgctcccg cataaaagtt ctccggtggg acaaacatgg cgtatggctg tgtacccgac
    12421 gactgcaccg gggaagcttc cgctggcccc ggaaaggtga tgccacatgg catctcacac
    12481 aggatgagtt tcactggctg gtctttgggg ttgactggca acaggtgaaa gggcatgacc
    12541 tggcaaaatg ggtataccag gaagaacccg aagatgctca gagcaacgca ataactcaat
    12601 gaaatataat gaatatccat gcgtgatgtc atataatcca ccgcatggat atctcgcttc
    12661 tcagtaccac cagtgaccct gaacagcttc gcgctctggc aattgccatg gtgcaaaaag
    12721 tcatggctga gaatgcagaa cttcagaacc gtatccgcat cctggaagag cagatgaagc
    12781 tcgcacgcca gcagcgtttc ggtaaaaaat gcgaatcgct ggccgggatg cagcgttccc
    12841 tgttcgaaga ggatgttgac gcggatatcg cagaaatctc ggcgcatctg gacaaactgc
    12901 ttcctcagac cggggacgaa gaaaaaacaa caacacgtcc tgttcgtaag cccttgcctt
    12961 cacctcttcc ccgtgctgaa aaggttatcc cgccagctga ggaacgttgt cctgactgtg
    13021 atgcccccct gcacttcatt cgcgatgaag tcagcgagaa gctggaatat attccggcac
    13081 aggtcgtggt caaccgctat atccgcccgc agtacagctg tccctgctgt gagaaagtgt
    13141 tcagcggaaa aatgccggca catatccttc cgaaaagtgc tgttgagcca tcggtcatcg
    13201 cacaggttgt catcagcaaa tataccgacc atctgcccct gtatcgccag cagcacattt
    13261 tctcccgaat gggggtggag ctccccgtca gtacaatggc ggatatggtc ggtgttgctg
    13321 gcgctgcact ggccccgctg gcaaaattgt tacgccacga gcttctgaca cgggatgtta
    13381 ttcatgcaga tgaaacgtcc ctgaggctcc ttgatacccg gaaaggcggt aaatcctgct
    13441 ccggctggct gtgggcttac gtcagcggag aaagagtaag cgtcaacgga gcaccgtatt
    13501 gacgcttatt tattggtgag tactacgttc catggcagga gttcatcaac tcggttggaa
    13561 ggccattctg gcagtacgct caggatatgg cgcagatacg cttccggatc gataccgttc
    13621 agacggcagg tgccgatcag cccgtacaac agtgctccac gctcgccgcc gtgatcgctg
    13681 ccaaagaaca taaagttttt ctttccgaga cagactgcac gaagcgctct ttccgcagca
    13741 ttattatccg cctccgccag accgtcatca ctgtaataac agagggcatc ccactgattc
    13801 agtacatagc tgcacgcttc gcccagtctg gattttttcg acagcgtacc attcttctcc
    13861 accatccatt catgcagtga cgtcagtaac actttgcttc gctgctgcct gacggcaaga
    13921 cgctctgact ccggtaatcc ccgtatttca tcctcgatgg cgtacagttc actgattcgc
    13981 ttcagggctt cttctgccgt cgcacttttg ctgctgatgt atacatcgtg gatttttcgc
    14041 cgggcatggg cccagcacgc aacttctgtc agtgcaccac cttcacgttc
//
LOCUS       DAZGJW010000122        13657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZGJW010000122 DAZGJW010000000
VERSION     DAZGJW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.122"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(23..211)
                     /gene="bcsR"
                     /locus_tag="ACHKIK_003696"
     CDS             complement(23..211)
                     /gene="bcsR"
                     /locus_tag="ACHKIK_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO9084790.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            484..2055
                     /gene="bcsE"
                     /locus_tag="ACHKIK_003697"
     CDS             484..2055
                     /gene="bcsE"
                     /locus_tag="ACHKIK_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO9084791.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            2052..2243
                     /gene="bcsF"
                     /locus_tag="ACHKIK_003698"
     CDS             2052..2243
                     /gene="bcsF"
                     /locus_tag="ACHKIK_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO9084792.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            2240..3919
                     /gene="bcsG"
                     /locus_tag="ACHKIK_003699"
     CDS             2240..3919
                     /gene="bcsG"
                     /locus_tag="ACHKIK_003699"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO9084793.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVSDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(4006..4113)
                     /locus_tag="ACHKIK_003700"
     CDS             complement(4006..4113)
                     /locus_tag="ACHKIK_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO9084794.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(4489..4596)
                     /locus_tag="ACHKIK_003701"
     CDS             complement(4489..4596)
                     /locus_tag="ACHKIK_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO9084795.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(4972..5079)
                     /locus_tag="ACHKIK_003702"
     CDS             complement(4972..5079)
                     /locus_tag="ACHKIK_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO9084796.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(5455..5562)
                     /locus_tag="ACHKIK_003703"
     CDS             complement(5455..5562)
                     /locus_tag="ACHKIK_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO9084797.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            6038..7309
                     /locus_tag="ACHKIK_003704"
     CDS             6038..7309
                     /locus_tag="ACHKIK_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO9084798.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(7339..8343)
                     /gene="dppF"
                     /locus_tag="ACHKIK_003705"
     CDS             complement(7339..8343)
                     /gene="dppF"
                     /locus_tag="ACHKIK_003705"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO9084799.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSFSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(8340..9323)
                     /gene="dppD"
                     /locus_tag="ACHKIK_003706"
     CDS             complement(8340..9323)
                     /gene="dppD"
                     /locus_tag="ACHKIK_003706"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO9084800.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDIYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(9334..10236)
                     /gene="dppC"
                     /locus_tag="ACHKIK_003707"
     CDS             complement(9334..10236)
                     /gene="dppC"
                     /locus_tag="ACHKIK_003707"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO9084801.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(10246..11265)
                     /gene="dppB"
                     /locus_tag="ACHKIK_003708"
     CDS             complement(10246..11265)
                     /gene="dppB"
                     /locus_tag="ACHKIK_003708"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO9084802.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRLQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(11416..13023)
                     /gene="dppA"
                     /locus_tag="ACHKIK_003709"
     CDS             complement(11416..13023)
                     /gene="dppA"
                     /locus_tag="ACHKIK_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO9084803.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(13375..>13657)
                     /locus_tag="ACHKIK_003710"
     CDS             complement(13375..>13657)
                     /locus_tag="ACHKIK_003710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084804.1"
                     /translation="SVASGNGTALIVGCGATALFDQHTTHFPANFAPHISLSINGFVT
                     ETIVFFYICLNLFLRYLWLALAFSAVRFSVHGINRSDIHLFLRFFSLNA"
BASE COUNT         3161 a         3668 c         3417 g         3411 t
ORIGIN      
        1 agtacggcca ttcaccaatc ccctactttt gttgcgcaaa ctctgccagc aacggccagc
       61 gttttaatgc cgcggccaac tgttcgcgtt gggaaatatc ggcataatca atatcaggca
      121 gtgaaaatgc ctgctttaac gccacaatat cattctggaa gatatagcct atcgcgggat
      181 caggcagagt atctggttca ttgttattca ttttgcttga tccctatgaa ataaccgtac
      241 cgttcatgag acaattctaa ccgtgagcat ttcgccggtt tattgtttac ggcgagacgg
      301 gaatttgctc actttttgac tttccgttac gcaacagaaa aagttaaaaa cgaaaagact
      361 aaaatcacac gtcttgtgac cttctaaatt gattcacatg ctaaatctga taagttttaa
      421 tttcaatggt aggtttattt cttagctttc gctagtaaac tgataatcag acaaaatggt
      481 gacatgaggg acattgtgga ccctgtattc tctatcggta tctcatcatt atgggatgag
      541 ctgcgacata tgccagcagg cggcgtctgg tggtttaacg tcgatcgcca tgaagatgct
      601 atcagtctgg cgaatcaaac aattgcatcc caggctgaaa ccgcacacgt cgcggtcatt
      661 agcatggaca gcgatccggc gaaaatcttt caattagatg attctcaagg gccggaaaaa
      721 ataaaattat tttcaatgct aaatcatgaa aaaggtctat actatttgac ccgtgatttg
      781 cagtgttcta ttgatcccca taattacctt tttattcttg tttgcgcaaa taatgcatgg
      841 caaaacattc ctgccgagcg gcttcgctca tggttggata aaatgaataa atggagcagg
      901 ttaaaccatt gttcgctttt ggtaattaat cccggaaata ataacgataa acaattttca
      961 ttgttgcttg aggaataccg ttcacttttt ggtcttgcca gtttgcgttt tcagggtgac
     1021 caacatttgc tggatattgc cttctggtgc aacgaaaaag gggtcagcgc ccgtcagcag
     1081 cttagcgttc agcaacaaaa tggtatctgg acattagttc aaagcgaaga ggcggagatc
     1141 caaccacgca gcgacgaaaa acgcattctg agtaatgttg ctgtactgga aggtgcgccg
     1201 ccgctatcgg aacactggca actgttcaac aataacgaag tcctgttcaa tgaagcccgt
     1261 accgctcagg cggcgacggt ggtcttttct ttacagcaaa atgcgcaaat cgagccactg
     1321 gcccgcagca ttcataccct gcgtcgccag cgcggtagtg cgatgaaaat cctcgtgcgg
     1381 gaaaataccg ctagcctgcg cgccaccgat gaacgtttgt tattggcctg cggtgcaaat
     1441 atggttattc cgtggaatgc gccactctcc cgttgtctga cgatgatcga aagcgtgcaa
     1501 gggcagaagt ttagtcgcta tgtgccggaa gatatcacta ccttgctgtc aatgacccag
     1561 ccgctcaaac tgcgtggttt ccagaagtgg gatgtgttct gtaatgccgt caacaacatg
     1621 atgaataacc ctctattacc tgcccacggt aaaggcgttc tggttgccct acgtccggta
     1681 ccgggtatcc gcgttgaaca agccctgacg ctgtgtcgcc ctaaccgtac cggcgatatc
     1741 atgaccattg gcggtaatcg gctggtgctg tttctctcat tctgtcggat taacgatctg
     1801 gataccgcgt tgaatcatat tttcccattg cctactggcg acattttctc aaaccgtatg
     1861 gtctggtttg aagatgatca aatcagtgcc gagctagtgc agatgcgctt gcttgcccca
     1921 gaacaatggg gcatgccgct gcctttaacg caaagttcta aaccggtcat caatgccgag
     1981 cacgatggtc gccactggcg acgaatacca gaacccatgc gactgttaga tgatgctgtg
     2041 gagcgctcat catgatgacc atcagcgata tcattgaaat tattgtcgtt tgcgcactga
     2101 tatttttccc gctgggctat ctggcgcggc actctttgcg acgcattcgc gacaccttac
     2161 gtttgttctt tgctaaacct cgttatgtta aaccggccgg gacgttacgc cgcacggaaa
     2221 aagccagggc aaccaaaaaa tgactcaatt tacgcaaaat accgccatgc cttcttccct
     2281 ctggcaatac tggcgcggcc tttccggctg gaacttctat tttctggtta agttcggcct
     2341 gttgtgggcg ggatatctta acttccatcc gctcctcaat ttggtgtttg ccgcgtttct
     2401 gctgatgccc attccgcgct acagcctgca tcgcttgcgc cactggattg ccctgccgat
     2461 cggctttgct ttgttctggc atgacacctg gttgcctggc ccggaaagca taatgagcca
     2521 gggttcgcag gtggcggggt tcagtaccga ttatttaatc gaccttgtca cacgctttat
     2581 taactggcag atgattgggg ccatttttgt tttattagtg gcctggttat tcctgtcaca
     2641 atggattcgc attaccgttt ttgtggttgc catactgcta tggctgaacg tacttaccct
     2701 ggcgggacca agtttctcct tgtggccagc cggacaaccg acgaccactg taacaacgac
     2761 gggtggtaac gcagcggcaa ccgttgcggc gacgggtggc gcaccggtag tgagtgatat
     2821 gcccgcacaa actgcaccgc caacaacggc gaaccttaac gcctggctga ataatttcta
     2881 taacgcggag gcaaaacgta aatcgacctt cccgtcttcg ctgcccgctg atgctcagcc
     2941 atttgaacta ctggtgatta acatctgttc gctttcctgg tcggatatag aagccgccgg
     3001 gttgatgtcg catccactgt ggtcgcattt cgatattgag ttcaagaact ttaactccgc
     3061 cacctcctac agtggcccgg cggcgatccg tttactgcgc gccagctgcg ggcagacttc
     3121 gcacactaat ctgtatcaac cggcaaataa cgactgctat ctgtttgata acctttcgaa
     3181 actgggcttt acccagcacc tgatgatggg gcataacggc cagttcggcg gttttttgaa
     3241 agaagttcgc gaaaatggcg gcatgcagac tgaattgatg gatcaaacaa atctgccggt
     3301 tattttgctg ggctttgatg gttcgccggt ttatgacgat accgccgtgc ttaaccgctg
     3361 gctggacgtt accgaaaaag ataaaaatag ccgtagtgcc acgttctaca acacgcttcc
     3421 actgcatgac ggcaaccatt atccgggggt cagcaaaaca gcggattaca aagcgcgggc
     3481 gcagaaattc tttgatgaac tggacgcctt ctttactgaa ctggagaaat cgggtcgtaa
     3541 agtgatggtg gtcgtggtgc cggaacacgg cggcgcgctg aagggcgaca gaatgcaggt
     3601 atctggccta cgtgatatcc ctagcccgtc tatcacagac gtccccgttg gggtgaaatt
     3661 cttcggcatg aaggcaccac atcagggggc accgattgtc attgaccaac cgagcagctt
     3721 cctggctatc tccgatctgg tggttcgcgt tcttgatggc aagattttca ccgaagacaa
     3781 tgttgactgg aaaaaactca ccagtgggtt gccacaaaca gcaccggtct ccgagaactc
     3841 aaatgcagta gttattcaat accaggataa accgtacgtt cgcctgaacg gcggcgactg
     3901 ggtgccttac ccgcagtaag cacgaaaaag gccgcagagt ttaacctctg cggcctgatc
     3961 cgggcgcaaa ttgccattac ggcagcctga cgcccgcatg acacgttact tccgcttatt
     4021 cagccagttc acgatcatac tggcaagaat gccagcaatg accggagccg ctaaatcatg
     4081 ccagaaggcc atgcccagct ctgcgagcgt catatagccg cctgtgttgt aatgacaacg
     4141 tttcgcggct attcttgagt tgtttgggtt caagattagc ccccgttatg ttgtcaggtg
     4201 catacctgca acgtgcgggg gttttctctc tccagcaacc aatgccacca gggataaagc
     4261 ccccgcaaca ttgcgcctca ccggaacctt tccggcttgc cgctgattct acgactgttt
     4321 ttctgttccg aaatccatca cttactcact ctgtgctgcg cattccgcag aatattatcc
     4381 gcttgcaacc gttgcatttt tcttacgaaa aaggccgcag agtttcaccc ctgcggcctg
     4441 gtccgggcgc aaattgccat tacggcagcc tgacgcccgc atgacacgtt acttccgctt
     4501 gttcagccag ttcacgatca tactggcaag aatgccagca atgaccggag ccgctaaatc
     4561 atgccagaag gccatgccca gttctgcgag cgtcatatag ccgcctgtgt tgtaatgaca
     4621 acgtttcgcg gctattcttg agttgttcag gttcaagaat ggcccccgtg atgttgtcag
     4681 gtgcatacct gcaacgtgcg ggggttttct ctctccagca accaatgcca ccagggataa
     4741 agcccccgca acattgcgcc tcaccggaac ccttccggct tgacgctgat tctacgactg
     4801 tttttctgtt ccggaatccg tcacgtcttc actctgtgct gcgcattccg cagaatatta
     4861 tccgcctgca accgttgcat ttttcttacg aaaaaggccg cagagtttca cccctgcggc
     4921 ctgatccggg cgcaaattgc cattacggca gcctgacgcc cgcatggcac gttacttccg
     4981 cttgttcagc cagttcacga tcatactggc aagaatacca gcaatgaccg gagccgctaa
     5041 atcatgccag aaggccatgc ccagttctgc gagcgtcata tagccgcctg tattgtaatg
     5101 acaacgtttc gcggctattc ttgtattgtt tggatgcaag aatggccccc gtggtgttgt
     5161 caggtgcata cctgcaacgt gcgggggttt tctctctcca gcaaccaatg ccaccaggga
     5221 ttaagccccc gcaacattgc gcctcaccgg aatccttccg gcttgacgct gattctacga
     5281 ctgtttttct gttccggaat ccgtctcatc ttcactctgt gctgcgcatt ccgcagaata
     5341 ttatccgctt gcaaccgttg catttttctt acgaaaaagg ccgcagagtt tcactcctgc
     5401 ggcctggtcc gggcgcaaat tgccattacg gcagcctgac gcccacatga cacgctactt
     5461 ccgcttgttc agccagttca cgatcatact ggcaagaatg ccagcaatga ccggagccgc
     5521 taaatcatgc cagaaggcca tgcccagctc tgcgagcgtc atatagccgc ctgtgttgta
     5581 atgacaacgt ttcgcggcta ttcttgagtg gtctaggttc aagattagcc cccgttatgt
     5641 tgtcaggtat atacctgcaa cgtgcggggg ttttctctct ccagcaacca atgccaccag
     5701 ggataaagcc cccgcaacat tgcgcctcac cggaaccctt ccggcttgcc gctgatttta
     5761 cgattgcttt ttactgtcag cacccggcaa tcttcctcat tgcgcgatgc cttccagctt
     5821 aatttcttta tttgcaatag ttgcagttca tatccgggct gactccagac tggtagtgaa
     5881 atgcgcaatt ttgtagaatt gcgcctcctc gcgacctccc cagtgaatct gttgcctcat
     5941 tttttacata ctgcgtattc gacttctcca cctgttgcgc aagagaaact gggtttattc
     6001 atttttgcga gaccgacttc tttctggaca ggactttatg cagcacaaca cactatcgaa
     6061 acacaatcag aaattgccgt ttacacgcta cgacttcggc tgggttttat tatgcatagg
     6121 catggcgatt ggtgccggaa ccgtgctgat gccagtacaa attggcttga agggaatttg
     6181 ggtatttatt accgcagcga tcattgctta tcctgccacc tgggtagtgc aggacattta
     6241 tttaaaaacc ctttctgaaa gcgattcctg taatgactac accgatatta tcagtcatta
     6301 cctggggaag aactggggaa ttttcctcgg ggttatctac tttttgatga ttatccacgg
     6361 gatttttatc tactctctct ccgtggtttt cgacagcgcc tcgtacctga aaaccttcgg
     6421 tttaaccgat gccgatcttt cacaatctct actttataaa gtcgctattt tcgccgtact
     6481 ggtggcgatt gcgtctggtg gtgaacgatt actgtttaag atttccgggc caatggtggt
     6541 ggtcaaagta gggattattg tcgtgttcgg ttttgcgatg atcccgcact ggaatttcgc
     6601 caatataacc gccttcccgc aagcctccgt ctttttccgc gatgtcctgc ttacaattcc
     6661 attttgcttc ttttctgcgg tatttattca ggtacttaac ccaatgaata ttgcctatcg
     6721 taaacgggaa gcagataaag tactggcaac ccggctcgcg ctgcgtaccc accgaattag
     6781 ttatatcacg ctcatcgcgg tgatcctgtt ttttgccttt tcgtttacct tctcaattag
     6841 ccacgaagaa gccgtttctg cctttgaaca aaatatctcg gcactggcgc tggccgcgca
     6901 ggtgatccct ggacatatca ttcatatcac ctctacggtg ctgaatatct ttgccgtact
     6961 gaccgcattc tttggcattt atctcggttt ccacgaggct attaaaggca ttattctcaa
     7021 tctgttaagc cgaattattg ataccaagaa aattaactca cgcatgctga ctctggcgat
     7081 ctgcgctttt atcgtcatta cattgacgat ttgggtttcg tttcgtgtat cggtgctggt
     7141 gttctttcag ttgggaagcc cgttatatgg cattgtgtcg tgcctcattc cgtttttcct
     7201 gatctataaa gtcgcacaac tggaaaaact tcgcggattt aaagcctggc taattctgct
     7261 ttacggcatt ttgctatgct tgtcgccact gttgaagctg attgagtaaa ccggagcgca
     7321 tggccccggt tttgtgagtt aacgctgcgg attttcatcc tgatcaacag caaaacaagc
     7381 gaccagttga ccgccgtagt cttttagctg cggctgcaac tgggtgcagg ggccgaagcg
     7441 ccgacgacag cgggcgttga aggcgcaacc cggcggagga ttcagcgggc ttggcagttc
     7501 accggtgagt ttgatgcgtt cacgacgatc gtccgggttc aggcgtggcg tcgcggagag
     7561 cagcgcctgg gtgtacggat ggcgcgggtt attgaagatt tggtccttcg ttcctttctc
     7621 cacgcagcgg cccaggtaca tcaccatcac ttcatcagca atgtgctcca ccaccgacag
     7681 gtcgtgggag ataaagacat aagaaagccc caactcctgc tgcaaatcca tcatcagatt
     7741 cagcacctgc gcacgcactg agacgtccag cgcagaaacc ggttcatcgg caatcaccac
     7801 gtccgggtcg agcatcagac cacgggcaat agcgatacgc tgacgttgtc cgccggagaa
     7861 catatgcgga tagcggtcat agtgctcggt tttcaggccg actttcgcca tcatcgacag
     7921 ggctttttcc cgacgctgtt ctttgctgaa gctggtgtta ataagcagcg gctcttcgag
     7981 aatttgccca acttttttac gcggattcag cgaaccgtaa gggttctgga agacaatctg
     8041 gattttctgc cgacgcagct tctgcgcctg cggatcgtgc ttgagcagat cctgcccctg
     8101 gtaatacagc tcaccaccgg tgggcgtttc gatcatcgtc agcaaccgac cgagggtcga
     8161 tttaccgcag ccagattcgc ccactactgc cagcgttttg ccacgttcaa ggttaaacga
     8221 aacgccgtcc agcgccttca ccagacgttc cggcgcgaac atgcctttct tcaccggata
     8281 atgttttttc aggtcgatag cctgcaacag cggttgttgc gaggtggcct cttgcgtact
     8341 catagtgtcg gcctcccggc atcatcaagt gggtaatggc atttggactg acgcccatca
     8401 gcgagcatat tcagcgccgg ttcttcagcg cgacatctgt cagtggcata ggggcagcgc
     8461 gggttaagca ggcagccgtt cgggcggtcg tacttaccgg gaacgacacc tggcaacgac
     8521 gccagacgtt ctttgtcctg agcaaattct ggcagcgcac gcagcaatgc ctgagtatac
     8581 gggtgacgcg gcgcatggaa gatggcgtgc gcatcaccgg tttccaccac ctggcctgca
     8641 tacatcacga tgattttatg tgccgcttcc gccaccagcg ccaggtcatg ggtaattaac
     8701 accagcgcca tgttctcttt ctgctgtagc tccagcagta gttcgatgat ttgcgcctga
     8761 atggtcacgt ccagcgcggt ggtcggttca tcggcaatca gcagttttgg ccgacaggca
     8821 atcgccatgg cgatcatcac gcgctggctc atgccgccgg aaagctgatg cgggtaaata
     8881 tccagacgcg atgccggatc gggaataccg acctgattca gcaggtcaat cgcccgctga
     8941 cggcgagtgc tcttattacc gccctgatgc accttaatcg cttccatgat ctggaaaccc
     9001 acggtgtagc acgggttaag gctggtcatc gggtcctgga agatcatcgc cacttcggca
     9061 cccaccaggt tgcggcgctc tttttctgat atacgctgca aatcctgacc gttaaacacc
     9121 agcttttccg ccattacgcg gcccggataa tcaatcagcc ccataatcgc cagtgaactg
     9181 accgacttac cggaaccaga ctcacccaca atcccgacca cttcgccctg ttttacgctg
     9241 tagctgatgc ggtctacggc gcggaacggt gcgctttcgt cgccgaaatg caccgataat
     9301 ttatctacat ttaataacgc catctcgaac ctcttactgc ttcagtttgg gatcgagcgc
     9361 gtcacgcaga ccgtcaccca tcaggttaaa tgccagcacc gtcagcagga tcgccagacc
     9421 cgggaaggtc acgacccacc aggcgctttg cgcgaactgc aacacgtcgg agagcatggt
     9481 gccccactct ggtgttggcg gctgcgctcc catgccgagg aagccaaggg cggccatatc
     9541 gagaatggcg ttagagaaac cgagcgacgc ctgaacaatc agcggcgcaa ggcagttcgg
     9601 gaagatgtta ataaacatct gacgcatcgc cccggcaccc gccacgcgag acgcggtgac
     9661 gtaatcgcgg ttaacttcca ccagcacggc ggcgcgggtt aagcgcacat agtgcggcaa
     9721 ggcaacgaag gtcagtgcca gcgcggcgtt accaatcgac gggccgaaga ttgccaccag
     9781 caccagcgcc agcagcagac ttggcagcgc cagcatgata tcgaccacgc gcataatgat
     9841 gttatcgacc aggccgccaa agtaaccggc gatcaggccg agaataacgc ccataatcag
     9901 cgataacaca actaccagac agccaaccag cagcgacagg cgcgcaccgt acatcaggcg
     9961 cgacagcaca tcacggccta cgtcatcggt gcccagcaag tgcgccatgc tgccgccttc
    10021 ctgccaggct ggcggggcga gcagtgcatc gcggaactgt tccgccgggt tatagggtgc
    10081 aatccagttg gcaaagatcg cgatgaacag cacgatgacg acgtaaacca gcccgacgac
    10141 cgcgcctttg ttgcgtttaa aatagtgcca gaactcctgt aacggggtca tcggcaccgg
    10201 tgcgctaatc actttatttt cagtaacctg tgacatgatg tccccttact tcttatgacg
    10261 aatacgcggg ttcaccacgc cgtacagcag atcgaccagc aagttgacga ggataatcat
    10321 cgtcgccacc agcaatacgc cgccctgcac taccggatag tcgcggcgtt gcagcgcgtc
    10381 aatcaaccag cgccccagac cgggccacga gaagatggtt tcggtcagaa tcgcccccgc
    10441 cagcaatgtt cccacctgca ggccgataac ggtcactacc ggcagcatcg cgttacgcag
    10501 cgcatggacg ataatcaccc gcatgcgggt tagccctttg gcgcgcgcgg tgcggatgta
    10561 atcctcgccc agcacttcca gcatcgagga gcgtgtcata cgcacaatga ccgccagcgg
    10621 aatagtaccc agcacaatgg caggcaagat catatgggcg acggcatcga taaagttgcc
    10681 gtcttcaccc cagatggcgg tgtcgattag cataaaaccg gttaacggat tggagtcatc
    10741 gaggaacacc atatcgctca cgcgaccgga gacgggcgtc aggttccagt gcaccgaaac
    10801 cagcatgatc agcatcatgc cccaccagaa gatcggcatc gagtagccgg tcagcgccag
    10861 gccaaccgct gtgtgatcga aaatggaacc gcgtttaacc gcagccagca cgccgaccgg
    10921 aataccgacc gcagtcgcaa aaatcatcgc gcagacgcca agttccagcg tggcctggag
    10981 gcgcggcacg aactcttccc aaaccgggat gcggcttttc attgaaatgc ctagatcgcc
    11041 atgcataacg ccccaaatgt aatggagata ctgctgccac atcggtttat ctaagccgag
    11101 ttcagccagc agctgcgcgt gacgctctgg ggagatccca cgttcgcccg ccatgatcat
    11161 caccggatcg cccgggatca tgtggacaaa ggcaaatgtg agaagggtaa taccgataaa
    11221 cgtggggatg acgagtccca aacgtcggag aataaactgc aacataaccc ggattctcta
    11281 tagtgacccg cagcctggaa ctgcgtgtct gtattgctca caaatcaatt cccgcctagg
    11341 tcgggctggg aatgaagtat tgctgccgga tggcgctgca cgcagaggca cagcgccacc
    11401 gggtatggct tttaattatt cgatagagac gttttcgaag tgatgtttgc ctaatggatc
    11461 aaccacatag cctttaactt ctttgcgtac cggttcaaac acggtggagt gagcgatgat
    11521 cagtgccgga gcctgatcgt gcatcaccac ctgcgcttgc ttgtacagtt caacgcgttt
    11581 gttatggtcg tcggtagcac gcgccggttg aatcagatct tcaaacggtt tgtagcacca
    11641 ttttgagtag ttggagcctt gttcagaggc ggcgcagctg aacagggtgg cgaagaagtt
    11701 atccggatcc ccgttatcgc cagtccagcc catcattacc gtctggtgct cgccatcttt
    11761 cgcacgcttg aggtactcac cccattcgta ggtgacaatt ttggcctgca cgccgacttt
    11821 cgcccagtca gcctgaatca tctccgccat gcggcgagcg ttcgggttat atgggcgctg
    11881 taccggcatc gcccacaggt cgatggagaa acctttttcc agacccgctt ctttcagcaa
    11941 ggctttcgct ttttcaggat cgtaggtgta atcctgaacg tcgtcgttat agccccacat
    12001 ggttggcggg atcaggtttt tcgctgatac acccgcgccc tgataaaccg ctttgatgat
    12061 cgcgtctttg ttcaccgcgt aggtcagtgc ctggcgaact ttcacgtcat ccagcggttt
    12121 tttctgcacg ttataggaga ggtagccgac gttcagcccc ggcatttcca tcaggttgat
    12181 ggatttatcc tgcttcatgc gagcgatgtc tgccgggttc gggtacggca tcacctggca
    12241 ttcattcttc tgcaatttcg cataacgcac ggaagcgtca ggagtaatag agaagaccag
    12301 cgtatcgatc tgcggtttgg tgccccagta gccatcaaac gctttgtagc ggatacggga
    12361 gtctttttgg tactgctgta actggaacgg accggtgccg attgggttga ggtccagttt
    12421 ttccggtgta ccggctttca tcatcgcgtc agcatattct tttgacagaa tagaggcgaa
    12481 gtccattgcc aggtcagcga ggaacggcgc ttccgggcga gtcagcacaa actgaacggt
    12541 gttgtcgtcc acctttttca cttcgctgat cagctccggc aagcccatgc cttcgaagta
    12601 ttcgtagctg ccgccagaaa ctttatggta cgggttttgc gcgtttttct gacgatcaaa
    12661 cgagaacacc acgtcatcgg cgttcagttc acgcgtcggt ttgaattctt tattgtcgtg
    12721 ccacttcaca cctttacgca gatggaaggt ataggtttta ccgtcttcgc tgacttccca
    12781 cttttcagcg aggcccggga tcacttcagt ggtgccgatt ttaaattcaa ccaggcggtt
    12841 ataaagcggt acggaagagg cgtcataggt ggtgccggag gtaaacagct gcgggttaaa
    12901 cccttccgga gatccttctg agcaataaac cagagtttta gcctgaacac ttgctgcgac
    12961 ggtcatagcc accaggctga gaccaagctt cagcatccct gactttttca aggaaatacg
    13021 cattattctg ctccaattgt gatgtttgtt gttttaaccc tttgcagtgg gttgtcgctg
    13081 cctgaccttt tttgtttttt gcccggtcgg gtcaacgtta tgaggtgggg atgccgtact
    13141 aattaacatc agtgtaacag tgcggatcct ccataaaatg cccctcgtga cctacaatct
    13201 gtcaacagaa tgtgaaaacg tcaatacagc caaccgggat ttacaccaac ggtgagaatc
    13261 cacaaacaaa gattaaaaaa acttcaaaca gctatttgca gcagagaaat ttgtgctact
    13321 ccaacatgac cagaacaatc agcttaatat ttagcaacat ttggtgatta agttttatgc
    13381 atttaatgaa aaaaatctga ggaaaaggtg gatatctgag cgattaatac catgaacgct
    13441 aaaacgcaca gcggaaaatg ccagcgccag ccataagtaa cgcaaaaaaa gatttaagca
    13501 aatataaaaa aagacaatgg tttctgtcac aaatccgtta atggatagtg agatatgggg
    13561 cgcaaagttg gcaggaaaat gtgtggtgtg ttggtcgaat aaggcggtcg cgccgcatcc
    13621 gacaataagt gcggtgccgt tgcctgatgc gacgctg
//
LOCUS       DAZGJW010000123        13492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZGJW010000123 DAZGJW010000000
VERSION     DAZGJW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.123"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..278
                     /locus_tag="ACHKIK_003711"
     CDS             <1..278
                     /locus_tag="ACHKIK_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFO9084805.1"
                     /translation="SSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESG
                     EVVDMAAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            278..5548
                     /gene="traI"
                     /locus_tag="ACHKIK_003712"
     CDS             278..5548
                     /gene="traI"
                     /locus_tag="ACHKIK_003712"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO9084806.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYMDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQTRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHTEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVMRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAV
                     GEMRSAGVDAQTQASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMQNGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRADITEREAALPESVLRESQREQEAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5568..6314
                     /gene="traX"
                     /locus_tag="ACHKIK_003713"
     CDS             5568..6314
                     /gene="traX"
                     /locus_tag="ACHKIK_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFO9084807.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWFQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            6369..6929
                     /locus_tag="ACHKIK_003714"
     CDS             6369..6929
                     /locus_tag="ACHKIK_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO9084808.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            7062..7274
                     /locus_tag="ACHKIK_003715"
     CDS             7062..7274
                     /locus_tag="ACHKIK_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084809.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAAHCHRRCRGRG"
     gene            7520..7981
                     /locus_tag="ACHKIK_003716"
     CDS             7520..7981
                     /locus_tag="ACHKIK_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO9084810.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            8027..8236
                     /gene="hha"
                     /locus_tag="ACHKIK_003717"
     CDS             8027..8236
                     /gene="hha"
                     /locus_tag="ACHKIK_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO9084811.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            8274..8864
                     /locus_tag="ACHKIK_003718"
     CDS             8274..8864
                     /locus_tag="ACHKIK_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO9084812.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGAAQQSPEHRS"
     gene            9104..9352
                     /locus_tag="ACHKIK_003719"
     CDS             9104..9352
                     /locus_tag="ACHKIK_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO9084813.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            complement(9369..9581)
                     /locus_tag="ACHKIK_003720"
     CDS             complement(9369..9581)
                     /locus_tag="ACHKIK_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001410080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFO9084814.1"
                     /translation="MAELLAIRFLDGPVNLLLVAKPGEDYRGFHFSGSCKSTSEPVPC
                     HLTAWPATKFLKRSAIYHAHQKQARM"
     gene            9599..9673
                     /gene="tap"
                     /locus_tag="ACHKIK_003721"
     CDS             9599..9673
                     /gene="tap"
                     /locus_tag="ACHKIK_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO9084815.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            9666..10523
                     /gene="repA"
                     /locus_tag="ACHKIK_003722"
     CDS             9666..10523
                     /gene="repA"
                     /locus_tag="ACHKIK_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO9084816.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANGEAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            11455..11619
                     /locus_tag="ACHKIK_003723"
                     /pseudo
     CDS             11455..11619
                     /locus_tag="ACHKIK_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            complement(11806..12993)
                     /locus_tag="ACHKIK_003724"
     CDS             complement(11806..12993)
                     /locus_tag="ACHKIK_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFO9084817.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRQVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVRFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICRQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHSWKNLHSYARKVMSMWRYRITRLLPRKYPELVIPDELAVEGNSKR
                     DWNRFLDSHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLVMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEEVVYAITE
                     TVRKTAIQIRWRGMYQRLLKVDPLKCILCGSQMRFTGLKRGYRLAELVLMHERLARQQ
                     VCG"
     gene            complement(12944..13357)
                     /locus_tag="ACHKIK_003725"
     CDS             complement(12944..13357)
                     /locus_tag="ACHKIK_003725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084818.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHPDDFFDEEDAEVLRFDN
                     FDDAVECCADLGIPFFLDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVTPPSDASPFCRYFSTGKPLA"
BASE COUNT         3246 a         3433 c         4001 g         2812 t
ORIGIN      
        1 tgtcttctgt catcaatgat aagaagtcag atgcaggtgt gagtgttccg gccgggggga
       61 tcgagcagga gctgaaaatg aaaccggaag aagagatgga acagcaactg ccacccggga
      121 tcagtgaatc cggtgaagtg gtggatatgg ccgcttatga ggcatggcaa caggaaaatc
      181 atccggacat ccagcagcag atgcagcgtc gtgaagaggt gaacattaat gtgcaccggg
      241 agcgcgggga ggatgttgag ccgggagatg atttctgatg ttgagtttca gcgttgttaa
      301 atcggcaggc agtgccggaa actattacac cgacaaggat aattactatg tgctgggcag
      361 catgggagaa cgctgggccg gtcagggggc tgaacagctt gggctgcagg gcagcgtcga
      421 taaggatgtt tttacccgtc ttctggaggg caggctgccg gacggggcgg atttaagccg
      481 catgcaggat ggcagtaata agcatcgtcc cggctacgac ctgaccttct ccgcccccaa
      541 aagtgtctcc atgatggcca tgttaggtgg cgataagcgc ctgattgatg cacataacca
      601 ggctgtggat tttgctgtcc gtcaggtgga ggcgctggct tccacacggg tgatgacgga
      661 cggacagtca gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accatgacac
      721 cagtcgcgat caggaccctc agttacacac gcatgtggtg gtggctaatg tcacgcagca
      781 taacggcgaa tggaagacac tgagcagtga caaagtgggg aaaaccggat tttccgagaa
      841 cgtgctcgcg aaccggattg cgttcgggaa aatatatcag agtgagttac gtcagcgcgt
      901 tgaggcgctg ggttatgaaa ccgaagtggt cggtaagcac ggtatgtggg agatgccggg
      961 cgtaccggtg gaagccttct ccggacgctc acaggctatc cgggaggccg tcggtgaaga
     1021 cgcctcgctg aaatcccggg atgtggcggc actggatacg cgtaaatcca aacagcacgt
     1081 cgatcctgaa gtcagaatgg ctgaatggat gcagacgctg aaggaaaccg ggttcgacat
     1141 ccgggcatat cgtgacgcgg cggatcagcg tgcggagatc cgcacgcagg cgcccgggcc
     1201 tgcttcacag gacgggccgg atgtgcagca ggcggtgaca caggcgattg ccggattaag
     1261 tgaacgaaaa gtgcagttca cgtacatgga cgtgctggcc aggacggtcg gcatactgcc
     1321 gccggaaaat ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga
     1381 gcagcttatc cccctcgatc gtgagaaggg gctgttcacg tccgggattc atgtgctcga
     1441 tgagctgtca gtccgggcac tcagtcgtga catcatgaaa cagacccggg tgaccgtaca
     1501 tccggagaaa agtgtccccc ggacggccgg ttacagcgat gccgtcagtg ttctggcaca
     1561 ggaccgtccg tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg
     1621 ggtggctgaa ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc
     1681 tgaccgtcgc tcgcagatga acctgaagca ggatgaacgg ctgtctggtg aactgataac
     1741 cggacgtcgt cagttgctgg aaggcatggc cttcacgccg ggcagtaccg ttatcgtcga
     1801 ccagggcgaa aaactctccc tgaaagagac gttaaccctg ctggacggtg ccgcacgtca
     1861 taacgtacag gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcgctgat
     1921 ggccatgaag gatgccgggg tgaacacata tcgctggcag gggggagagc agcgaccggc
     1981 caccatcatc agtgaaccgg accgtaatgt ccgctatgcc cggctggccg gagattttgc
     2041 ggccagcgtg aaagccggag aagagagcgt ggcacaggtc agcggggtac gggaacaggc
     2101 catactgaca caggccattc gcagtgagct gaaaacgcag ggcgtgctcg gacacacgga
     2161 ggtgaccatg accgccctgt caccggtctg gctggacagc cggagccgtt atctgcggga
     2221 tatgtaccgt ccggggatgg tgatggagca gtggaacccg gagacacgca gtcatgaccg
     2281 ctatgttatc gaccgggtga cagcgcagag tcacagcctg accctgcggg atgcgcaggg
     2341 cgaaacgcag gtgatgcgta tttcctccct ggacagcagc tggtcgctgt tccggccgga
     2401 aaaaatgccg gtggcagacg gcgagcgact gagggtgaca gggaaaattc ccggactccg
     2461 cgtctccggc ggtgaccgcc tgcaggtggc atccgtcagt gaagatgcga tgacggttgt
     2521 tgtgccgggg cgggctgaac cggccaccct gcctgtggct gattcaccat tcacggcact
     2581 gaagctggag aacggctggg tggaaacgcc cgggcattcc gtcagcgaca gtgcgacggt
     2641 gtttgcctcc gtcacacaga tggcaatgga caacgccacc ctgaacggtc tggcccgcag
     2701 cggtcgtgat gtccggctgt attcctcact ggatgaaacc cgtactgcgg aaaaacttgc
     2761 ccgccatccc tcctttacgg tggtttctga gcagataaag gcgcgggccg gtgagacatc
     2821 gctggaaacc gctatcagtc tgcagaaagc cgggctgcac acgccggcac agcaggccat
     2881 tcatctggcc cttcctgtgc tggaaagtaa aaacctggcc ttcagcatgg tggacctgct
     2941 gacagaggcg aagtcgtttg ctgcagaagg aaccggtttt actgaactgg gaggggaaat
     3001 caatgcgcag ataaaacgcg gtgacttact gtatgtggat gtggcaaaag gctatggcac
     3061 aggcctgctg gtttcccgtg cgtcgtatga ggcagaaaag agcattcttc gccatattct
     3121 cgaaggcaag gaggcggtca cgccgctgat ggagagagta cccggcgaac tcatggagac
     3181 gttaacgtcg ggacagcgtg ccgccacccg catgatactg gaaacgtccg accgtttcac
     3241 ggtggtacag gggtatgccg gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc
     3301 cgtgaacatg ctgccggcga gtgagcgtcc ccgtgtcgtt gggctggggc ccacgcaccg
     3361 tgcggtcggg gagatgcgca gcgccggcgt ggatgcgcag acacaggcgt cctttctgca
     3421 tgacacgcag ctgcagcagc gcagcggaga aacgccggat ttcagcaaca cgctgttcct
     3481 gctcgatgag agctcaatgg tgggcaatac cgacatggca cgggcatacg ccctgattgc
     3541 ggccggtggc ggtcgtgccg tggccagcgg tgacacggac cagctgcagg ccatcgcgcc
     3601 cggtcagcct ttccgtctcc agcagacgcg cagtgctgcc gatgtggcca tcatgaaaga
     3661 gattgtgcgt cagacgccgg aactgcggga ggcggtatac agcctgatta accgggatgt
     3721 ggaaagggcg ctgtccggac ttgagagtgt gaaaccgtct caggtgccac gtctggaggg
     3781 cgcatgggca ccggagcact ccgtgacgga gttcagtcac agccaggaag cgaaactggc
     3841 agaagcgcag cagaaggcga tgcagaacgg cgaggctttt ccggatgtcc ccatgacact
     3901 gtatgaagcc attgttcgcg actataccgg caggacaccg gaagcacggg agcagacgct
     3961 gattgtcacg cacctgaatg aggaccggcg cgtgctgaac agcatgattc atgatgcacg
     4021 ggaaaaggcc ggtgagctgg ggaaagagca ggttatggtg cctgtcctga acacagcgaa
     4081 tatacgcgac ggggaactgc gtcgtctctc cacctgggag acacatcggg acgcacttgc
     4141 cctggtggat aatgtgtatc accggattgc cggtatcagt aaggatgacg ggctgataac
     4201 cctggaggat gcggaaggta acacgcggct gatttcgccc cgggaggcgg tggctgaagg
     4261 cgtcacactg tacaccccgg acaccatccg ggtggggacc ggtgaccgga tgcgcttcac
     4321 gaagagtgac cgggagcgcg gctatgtggc caacagcgtc tggacggtga cagcggtttc
     4381 cggtgacagt gtcacgctgt cggacggaca gcagacccgg gtgattcgcc ccggccagga
     4441 gcaggcagag caacatattg acctggccta tgccatcacc gcccacggtg cgcagggggc
     4501 gagtgaaact tttgccatcg cgcttgaagg cacggaaggt aaccggaaac tgatggccgg
     4561 ctttgagtca gcctacgtgg ccctgtcgcg tatgaaacag catgtgcagg tgtacaccga
     4621 taaccgtcag ggctggacgg atgccattaa caatgccgta cagaaaggaa ctgcccacga
     4681 tgtgctggag ccgaaaccgg accgggaggt catgaatgca gagcggctgt tcagtacggc
     4741 gcgggagctg cgggacgtgg cggcaggccg tgccgttctc cgtcaggcgg ggctggccgg
     4801 gggagacagt cctgcacggt ttattgcacc ggggcgcaaa tatccacaac cgtatgtggc
     4861 actgccggcg tttgaccgta acggcaggtc cgccggtatc tggctgaacc cactgaccac
     4921 ggatgacgga aacgggctgc ggggattcag cggtgaagga cgggtgaaag gcagcgggga
     4981 tgcgcagttc gtggccctgc agggcagccg taacggagag agcctgctgg ctgataatat
     5041 gcaggatggt gtccggattg cccgtgataa tcctgacagc ggtgtggtgg taagaattgc
     5101 cggtgaaggt cgtccgtgga atcccggtgc catcaccggt ggtcgcgtgt ggggggatat
     5161 cccggacaac agcgtccagc cgggggccgg aaatggcgaa ccggtcacag ctgaagtgct
     5221 ggctcagcgg caggctgaag aggccatccg ccgtgaaacg gaacgccgcg cagatgaaat
     5281 tgtcagaaaa atggcagaga acaaacctga cctgccggat ggcaaaacag agcaggctgt
     5341 cagggaaatt gccgggcagg agcgtgaccg ggctgacata actgaacggg aagccgcgct
     5401 gccggagagt gtgctgcgtg aatcacaacg ggagcaggaa gcggtccggg aggttgcccg
     5461 ggaaaatctg ctgcaggagc gactgcagca gatggagcgg gatatggtcc gtgacctgca
     5521 gaaagagaaa accctgggcg gagactgata caggaagata aacgctgatg acaacggata
     5581 acacgaacac gacacgtaac gattcactgg ctgcacggac cgatacctgg tttcagtcat
     5641 ttctggtctg gtcacccgga cagcgggata tcatcaaaac ggtggcactg gtgctgatgg
     5701 tactggacca catcaatctg atattccagc tgaagcagga atggatgttt ctggccgggc
     5761 gaggagcctt tccgctgttt gccctggtgt gggggctgaa tctgtcccgt catgcgcata
     5821 tccggcaacc ggccattaac cggctgtggg gatggggaat tattgcccag ttcgcatatt
     5881 atctggcagg ctttccctgg tatgagggga atatcctgtt tgcctttgca gtggcggcac
     5941 aggtgctgac gtggtgtgaa acgcgcagtg ggtggcgtac agcggcggcc attcttctga
     6001 tggccctgtg ggggcctttg tccggcacca gttacggcat tgccgggctg ctgatgctgg
     6061 cagtcagcca ccggctgtac cgggcggaag acagagcgga acgtctggcg ctggtcgcct
     6121 gcctgctggc tgttattccg gcgcttaacc ttgccaccag tgatgcagcg gcggtggctg
     6181 gtcttgtgat gacggtgctg accgttggtc tggtgtcgtg tgcaggaaaa tcattacccc
     6241 gtttctggcc cggggatttt ttcccggtat tctacgcctg tcatctggct gtgctgggcg
     6301 ttctggcgct gtgacgggtg tggtatcttt ggccgcaaga ggatgattcg tcagaggcag
     6361 aacacagcat gacagagcag aagcgaccgg tactgacact gaagcggaaa acggaagggg
     6421 agacgcctgt ccgcagccgg aaaactatca tcaatgtcac cacgccacca aagtggaagg
     6481 tgaaaaagca gaagctggcg gagaaagcag cccgggaggc agagctggcg gcaaaaaaag
     6541 cgcaggccag acaggcgctg tccatttatc tgaacctgcc ctcgctggat gaggccgtga
     6601 acaccctgaa accctggtgg ccgggattat ttgacggtga cacaccccgg cttctggcct
     6661 gcggtatccg ggacgtgtta ctggaagacg tggcgcagcg gaatatcccg ctctcgcata
     6721 aaaaactgcg cagggcgctg aaggccatca cccgttcaga aagctatctg tgtgccatga
     6781 aagccggtgc ctgccggtat gacacggaag ggtatgtgac ggagcatatt tctcaggagg
     6841 aggaagcgta tgcggcagcg cgtctggata aaatccgccg ccagaaccgg ataaaggcag
     6901 aacttcaggc cgtgcttgat gagaaataaa aaaagcctcc ctcagagagg ggaggcaggg
     6961 aaataaggct taaaaggaaa taacttccgt acccgaaagg aagccaattc agtagattat
     7021 ttaacaccat aatactgacg ggttaaagag gagtgataaa gatgaatgga ttcagaaaca
     7081 gttcacggaa cggtcaggtc tggcgctggc agcgtgccgg aagccgggcc gttattctgg
     7141 aggtcagtgg acgctggatg gaagcggcag aagcatggcg acgggctgca ggtgttgcgc
     7201 cccggacaga ctggcagcag tttgcccgaa aaagggctgc acactgccac cggcgctgcc
     7261 ggggaagggg gtaacgaaaa aagcagcctt cattactcca gggattcaga aggctgaata
     7321 atgcatgaga tttgcttttt ttaatatccg gtcgggcagt atgaccggac agttctgagt
     7381 agcacagagt tttttccgta ttaagtcgtg attttatatt ttgtgattaa tttcacaaaa
     7441 taaggtgtta ttcagtgtgt gctgcgatat tcaggattac ctgaaataca ggtgccattt
     7501 atctgatatg gagaataaca tgaggaaata tattccactg gtattattta tcttttcatg
     7561 gccggtattg tgtgcagata ttcatggacg ggttgttcgt gtactggacg gtgataccat
     7621 cgaggtcatg gactcacgga aggcagtgcg gatcaggctg gtaaatattg atgcgccgga
     7681 aaagaaacag gattatggcc ggtggtcaac ggacatgatg aaatcactgg ttgcaggaaa
     7741 aacggttacc gtcacatatt ttcagcgaga tcgttatggt cgtattctcg ggcaggttta
     7801 tgcaccagac ggaatgaatg ttaatcagtt tatggtccgg gccggggctg cctgggtata
     7861 tgagcagtac aacacagatc ccgtgctgcc tgtccttcag aacgaagcca gacaacaaaa
     7921 acgtgggctc tggtcagatg ctgatccggt tcccccctgg atatggagac accgtaaata
     7981 aagcgatggc aatcttatca ttcatctgtt ctcagcggga ataaaaatgg cgaaaacaaa
     8041 acaggaatgg ctgtatcagt tacggcgatg ttcatcagtc aatacgctgg aaaaaattat
     8101 tcataaaaac agagacagtt tattaaacag tgaacgtgaa tcatttaatt cagcagcaga
     8161 ccaccggtta gcggaattaa tcaccggaaa gttatatgac aggataccta aagagatatg
     8221 gaaatatgta aggtaataat tcagcataat taattaccag gagcagataa acaatgaagc
     8281 tgattatttt tattctcatt gttctgatta ttgcagcgtt acttatccgg attatcctca
     8341 gaagtgtaaa tcagcattct cctttgctga tgcaattgca tgcggcaggt atacggaccg
     8401 gtgatgcaga gcgaatactg tcaggcggtg aatactggca acgtcagaag acgctgctga
     8461 cagaaaggga agtcagtttt atgaaaggac tgttcagaat tgtggatatg aagcggtggt
     8521 atctgtgtcc gcaggtacgg gtcgcagata tcgtccagct gaacgggaat atccggccac
     8581 gatcacgcca gtggtggcag ttattcagga tggtgtctca gtggcatgtt gatgtggtta
     8641 tcgttgagcg gcgttcgttc agtattgttg cagcagtaga gctggatgat gccagccatt
     8701 tacgacctga acgcagacgc cgggatattc ttctggaaga ggttctgagg caggctggta
     8761 ttccgttgct cagaagccac gatgccagaa aactgctgca gatgaccgga gaatggctga
     8821 atacaacagg ggctgcccag cagtcaccgg aacatcgtag ctgacgcctt cgcgttgctc
     8881 agttgtccaa ccccggaaac gggaaaaagc aagttttccc cgctcccggc gtttccataa
     8941 ctgaaaacca tactatttca cagtttaaat cacattaaac gacagtaatc cccgttgatt
     9001 tgtgcgccaa cacagatctt cgtcacaatt ctcaagtcgc tgatttcaaa aaactgtagt
     9061 atcctctgcg aaacgatccc tgtttgagta ttgaggaggc gagatgtcgc agacagaaaa
     9121 tgcagtgact tcctcatcag gtacaaagcg agcatacaga aaaggtaatc ctctgacgct
     9181 tgcagaacgt cagcaggcat ccctggcaag aaaacgagct acacataaag aactcagagt
     9241 gttcattcct gctgcgttaa aagcgcaatt gcaggtgatg tgtgaagctg agggcgttac
     9301 ccaggctgag atgattgccg aactgataaa gcagaagagt gcttttagct aactaaaatg
     9361 tagcgacatc acattcttgc ctgtttctgg tgtgcgtgat agattgctga tcgtttaagg
     9421 aattttgtgg ctggccacgc cgtaaggtgg cagggaactg gttctgatgt ggatttacag
     9481 gagccagaaa agtgaaaacc ccgataatct tcacccggtt tggcgactaa gagaagatta
     9541 acggggccat ctaaaaaccg tatagccaac aattcagcta tgcggggagt atagttatat
     9601 gcccggaaaa gttcaagact tctttctgtg ctcactcctt ctgcgcattg taagtgcagg
     9661 atggtgtgac tgatcttcaa caaacgtatt accgccaggt aaagaacccg aatccggtgt
     9721 ttacaccccg taaaggtgcc ggaacgctga agttctgcga aaaactgatg gaaaaggcgg
     9781 tgggcttcac ctcccgtttt gatttcgcca ttcatgtggc gcatgcccgt tcgcgtggtc
     9841 tgcgtcgacg catgccaccg gtgctgcgtc gacgggctat tgatgcgctc ctgcaggggc
     9901 tgtgtttcca ctatgaccct ctggccaacc gcgtccagtg ctccatcacc acactggcca
     9961 ttgagtgcgg actggcgacg gagtctgctg ccggaaaact ctccatcacc cgtgccaccc
    10021 gggccctgac gttcctgtca gagctgggac tgattaccta ccagacggaa tatgacccgc
    10081 ttatcgggtg ctacattccg accgacatca cgttcacacc ggctctgttt gccgcccttg
    10141 atgtgtctga ggatgcagtg gcagctgcgc gccgcagtcg tgttgaatgg gaaaacagac
    10201 agcgcaaaaa gcaggggctg gataccctgg gtatggatga gctgatagcg aaagcctggc
    10261 gttttgtgcg tgagcgtttc cgcagttacc agacagagct taagtcccgt ggaataaaac
    10321 gtgcccgtgc gcgtcgtgat gcgaacaggg aacgtcagga tatcgtcacc ctggtgaaac
    10381 gacagctgac gcgtgaaatc tcggaagggc gcttcactgc taatggtgag gcggtaaaac
    10441 gcgaagtgga gcgtcgtgtg aaggagcgca tgattctgtc acgtaaccgt aattacagcc
    10501 ggctggccac agcttctccc tgaaagtgac ctcctcagaa taatccggcc cgcgccggag
    10561 gcatccgcac gcctgaagcc cgtcagtgca caaaaaagca gcaccacata caaaaaacaa
    10621 cctcaccatc caccttctgg tgcatccggt tacccctgtt tttaatacaa aatacgcctc
    10681 agcgacgggg aattttgctt atccacatat aactgcaagt gacttcccca taaggttaca
    10741 accgttcatg tcataaagcg ccagccgcca gcgttacagg gtgcaatgta tcttttaaac
    10801 acctgtttat atctccttta aactacttaa ttacattcat ttaaaaagaa aacctgttca
    10861 ctgcctgtcc tgtggacaga cagatatgca cctcccaccg caagcggcgg gccccgaccg
    10921 gagccacttt agttacaaca ctcaaatgca accaccggaa aaaccccgga ccagcgcaga
    10981 actgaagcca caaagcccct ccctcataac tgaaaagcgc cgccgccccg gcccaaaggg
    11041 ccggaacaga gtcgctttta attatgaatg ttgtaactat actccatcat ggctgtcagt
    11101 attctggctg gaagtgttga atacacgctc gtaagcagcc ctgacggccc gctaacgcgg
    11161 agatacgccc cgacttcggg taaaccctcg tcgggaccac tccgaccgcg cacagaagct
    11221 ctgacatggc tgaaagcggg tatggtctgg cagggccggg gatgggtaag gtgaaatcta
    11281 tcaatccgta ccggcttacg ccgggcttcg gcggttttac tccggtatca tatgaaacaa
    11341 cagagcgccg ccttccatgc cgctcacgcg gcatcagtgc aatacaagca gcattttgtg
    11401 tattttcaaa agtacgagta ataaagactt gttaaacagt ctcaaaggtg cgttatgcct
    11461 aacatcatcc tgtctgacac cagtgcaagt gtcagtgaac tgaaaaaaaa tcctatggct
    11521 acagtcagtg ccggagatgg ttaccctgtc gccattctta accggaatca gccggcattc
    11581 tactgtgtgc ctgctgaact gtatgaaagg atgcttgatc gataggaatt taagtcccca
    11641 aaagactaaa aaagcaccac aaaacggatg tttcttcaac cccccttgtg caccatatga
    11701 gcggaaacga ctattaaaca ggatgacact gattgataca tggtatgaat ggcggttttt
    11761 tgctccgttc ccggtaaaat ggacgcaact tcccctctgc ggctctcagc cgcacacctg
    11821 ctgtcgtgcc agtcgctcat gcatcaggac cagctctgcc agtcggtaac cccgcttcag
    11881 ccccgtaaaa cgcatctgac ttccgcacag aatgcacttc agcgggtcaa ccttcagtaa
    11941 cctctgatac atccctctcc acctgatttg tatcgctgtt tttctcactg tctccgttat
    12001 ggcgtacacc acctcttcca gtaaccggcg cttcaccgga ctcaggaaac cgtagtacct
    12061 caccatacgg aaccccttat ctgctacatg ccaggagaac ctttccatga actcatcgcc
    12121 actcatcacc aggtattctt cccgttttgt ccggtggctg ttgtaacgca gaccgatttc
    12181 atcctgacct gcatagtgct ccagacggct catcggcacc ggcggctttt tcaggtaaga
    12241 gccaaagtac actgccacat gtgtggcgtt atccatcacc ctggatacgt ttacattcca
    12301 gccccgccgg taatgactgt ccaggaagcg attccagtcc cgtttgctgt ttccttccac
    12361 tgccagttca tccggtatta ccagctccgg gtatttccgg ggcagtagcc gcgttatccg
    12421 gtaacgccac atgctcatca ccttacgggc gtaagaatga aggtttttcc aggagtgacc
    12481 tgacgtcacg cctccggctg ttgtcgacag gtgaatgtgc ggatgccact gctggtctcg
    12541 tccccatgtg tggattaccg tgaatatccc cggctccaca tctgcctggc ggcagatttc
    12601 cagtatcaca tccgcagcaa tgcggctcat ctctgccagt aaccaccggt tgtggaacac
    12661 cagggaccag tactggcagg gaagtgtgaa cacaatatgc tgccacgggc agtcggggac
    12721 cagactcagc agatactgta tccactgtgc gccagccttc actccgcagt gcgggcagga
    12781 gcgactttta caccggaagc ggaccttttt tgtgtggcaa cagtccggtg atgaacagca
    12841 ccactgtgtg taccccatca gcgtggttcc gcacgccatg atttttgtca cagactcaat
    12901 gaccacctga cgtactgagc cttccggctg tttctccagc cagttaagcc agcggtttcc
    12961 ctgttgaaaa atatcggcaa aacggggaag catcagacgg gggggtaacg ccatccggcc
    13021 agtgaactgt gccgcattcc gggcagtaca taccaccggc actgataccg gcaagaatgg
    13081 ttgcaaactc ccgctccgta cagcgggcta tttccggata cccttcgtca tcgacacgaa
    13141 caaaccagaa gaccagcttt ttgtttcctg catccagaaa gaacggaata cccaggtcag
    13201 cgcagcactc aacggcatcg tcaaaattat caaagcgcag aacttcagcg tcttcttcgt
    13261 caaaaaaatc atccggatga agtttcacaa catagcggag aagtttgctt ctttgtggtg
    13321 cgggtttgcg tttacggggt ttagctgaac gagccatata accacctgaa agacaaggac
    13381 attatcattt tttataacgg taattccagg ccatgacaag ccgcagccgc caggctgcct
    13441 actcgagcga aatacgccgg gcatggaacc agagccgggg ctctgcaggc gc
//
LOCUS       DAZGJW010000124        13365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZGJW010000124 DAZGJW010000000
VERSION     DAZGJW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.124"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            572..1396
                     /locus_tag="ACHKIK_003726"
     CDS             572..1396
                     /locus_tag="ACHKIK_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HFO9084819.1"
                     /translation="MIKPFSVRYGHVDVREHVQLNDLNSDTRMALWNCLYLFLWTNNR
                     QTATATKCAQSVWIYYLNQPADNIPRYESGYKSDKTLLTAIRDYIYGEAWYLVYDLIE
                     FIIERTNSYINLSKHLNSIFKKHGVGYTIINGCITPISNDNEIESVQNAVDNGTDSSR
                     SHFERALQLMTDREQPDYRNSIKESISAIESLCRKITGNDKGTLGACLKAIEEKGYIH
                     SAMKGAFSQLYGYTSDQGGIRHALTEEDVNPTLAEAKFMLVTCSAFSNYLLSKISD"
     gene            complement(1445..2017)
                     /locus_tag="ACHKIK_003727"
     CDS             complement(1445..2017)
                     /locus_tag="ACHKIK_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084820.1"
                     /translation="MVDASAQPHIVVTLEPNPWAAFYFDINIANTGNAPAYNVEVVFD
                     PPLVNAEHREKSEIPFSKVSVLKNGQSLTSNLCKYEQIKDQIYNINISWASKPKSNDR
                     ETNEYVYDMATFEGISYLGARSPLTQIAEQIKGIREDWKPIAQGAKKVKADVYTSSDR
                     NEERTYLQEQHDLAIKRRDEKREKRLESGE"
     gene            2118..3288
                     /locus_tag="ACHKIK_003728"
                     /pseudo
     CDS             2118..3288
                     /locus_tag="ACHKIK_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3371..3628
                     /locus_tag="ACHKIK_003729"
     CDS             3371..3628
                     /locus_tag="ACHKIK_003729"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084821.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHKDIIDNNRSINWPV"
     gene            complement(4186..4953)
                     /gene="fecE"
                     /locus_tag="ACHKIK_003730"
     CDS             complement(4186..4953)
                     /gene="fecE"
                     /locus_tag="ACHKIK_003730"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFO9084822.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(4954..5910)
                     /gene="fecD"
                     /locus_tag="ACHKIK_003731"
     CDS             complement(4954..5910)
                     /gene="fecD"
                     /locus_tag="ACHKIK_003731"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFO9084823.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(5907..6905)
                     /gene="fecC"
                     /locus_tag="ACHKIK_003732"
     CDS             complement(5907..6905)
                     /gene="fecC"
                     /locus_tag="ACHKIK_003732"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFO9084824.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILASIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(6902..7804)
                     /gene="fecB"
                     /locus_tag="ACHKIK_003733"
     CDS             complement(6902..7804)
                     /gene="fecB"
                     /locus_tag="ACHKIK_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFO9084825.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(7849..10173)
                     /gene="fecA"
                     /locus_tag="ACHKIK_003734"
     CDS             complement(7849..10173)
                     /gene="fecA"
                     /locus_tag="ACHKIK_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFO9084826.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(10260..11213)
                     /gene="fecR"
                     /locus_tag="ACHKIK_003735"
     CDS             complement(10260..11213)
                     /gene="fecR"
                     /locus_tag="ACHKIK_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFO9084827.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(11210..11731)
                     /gene="fecI"
                     /locus_tag="ACHKIK_003736"
     CDS             complement(11210..11731)
                     /gene="fecI"
                     /locus_tag="ACHKIK_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFO9084828.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            11868..11936
                     /gene="ykiE"
                     /locus_tag="ACHKIK_003737"
     CDS             11868..11936
                     /gene="ykiE"
                     /locus_tag="ACHKIK_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFO9084829.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            12023..12720
                     /locus_tag="ACHKIK_003738"
                     /pseudo
     CDS             12023..12720
                     /locus_tag="ACHKIK_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
     gene            complement(12731..12802)
                     /locus_tag="ACHKIK_003739"
                     /pseudo
     CDS             complement(12731..12802)
                     /locus_tag="ACHKIK_003739"
                     /inference="COORDINATES: protein motif:HMM:NF013446.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12865..13011
                     /locus_tag="ACHKIK_003740"
                     /pseudo
     CDS             12865..13011
                     /locus_tag="ACHKIK_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            13038..13142
                     /locus_tag="ACHKIK_003741"
                     /pseudo
     CDS             13038..13142
                     /locus_tag="ACHKIK_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3208 a         3439 c         3557 g         3161 t
ORIGIN      
        1 ataggttaaa tgtgtgtgcg agtccggcct tcgcaccaag ttactaaatt ccttacaata
       61 cagttagtta gacgattaaa tctgtctctt ttgcctgtaa gaaatgctct gttatgtccc
      121 ttgaaaaagc cgctgtggtt gatagtggta attgggatgt taactcatag cggcgtgtcg
      181 ttccaagcgt caaacacgta ctgactcaac ttgaaagcta tgacgtagcc accgtctcta
      241 aacaatccca aaacttttag tgcagcgcat tttagcccga tgcccccatt ctgtcgccgt
      301 cgcgagacgc cgcaggggat aaacaggttt atggtggtca cggtacttgc agaccatatg
      361 caagctctgc cagcggttcg gcctcgctcc ccttgggtac atggtcatcc tgataagcct
      421 cactaactga cataaccctg atgaagtcgc aagaggctcc gcttgttgtc ttaccaccaa
      481 cggggcttta tggattctat tgcttaatat atgattgtat caaacttaga ctgcataatt
      541 taaacgaaca taacaatcag gcaggggaag aatgataaag ccattttctg tgaggtatgg
      601 tcatgttgac gttagagagc atgtccagct gaatgatctt aattctgata cacgcatggc
      661 attgtggaat tgcttatatc ttttcctttg gactaacaat cgtcaaactg ctacagcaac
      721 aaaatgtgcg cagtctgtgt ggatatatta tctaaatcaa cctgcggata atattcctcg
      781 ttatgaaagt ggatacaaaa gtgataagac gttgctgact gctatccgag attatatata
      841 tggggaagca tggtatttgg tatatgattt aattgagttc atcattgaga gaactaattc
      901 atatattaat ctatcgaagc atctcaattc catctttaaa aagcatggcg ttggttatac
      961 cataataaat ggttgtatta ctcctatttc aaatgataac gaaattgaaa gtgttcagaa
     1021 cgcagttgat aatggcactg attcatcgcg tagccatttc gaaagggcgt tacaactaat
     1081 gacggataga gagcagccag actacagaaa ctcgattaaa gagtctatct ctgcaattga
     1141 atctttatgc aggaaaatca cgggcaatga taaaggaacc ttgggggctt gtcttaaagc
     1201 tatagaggaa aaaggatata tacactctgc aatgaaaggc gcttttagtc agctctatgg
     1261 ttatacaagt gatcagggtg gaatacgtca tgcgctaaca gaagaagatg taaatcctac
     1321 cttagctgaa gctaaattta tgttagtcac ttgcagtgcg ttcagtaatt atctgttgtc
     1381 aaaaataagt gattaattac aggtttatag ttacgagtgg gttagttacc cactcccttt
     1441 aaaattattc accagactct aatctttttt ctcttttctc atctctcctt tttattgcca
     1501 aatcgtgttg ctcttgcaga tacgtgcgtt cttcgtttct atcgcttgaa gtatatacgt
     1561 ctgcttttac ttttttagct ccttgtgcaa taggtttcca atcctctctt atacctttaa
     1621 tttgttctgc aatttgcgtc aatgggcttc tcgctcctag ataacttatt ccttcaaatg
     1681 tcgccatgtc atacacatat tcatttgttt ctctatcgtt tgatttaggt ttgcttgccc
     1741 agcttatatt aatattataa atttgatctt tgatttgttc atacttacag agattgctgg
     1801 taagtgattg cccatttttt aacacgctta ccttactaaa cggaatctca cttttttctc
     1861 tatgctccgc atttactagt ggaggatcaa acacaacctc aacattatag gcaggtgcgt
     1921 tccctgtatt agcaatgtta atatcaaagt aaaaagcggc ccaagggttt ggctcaagtg
     1981 ttacaactat atggggttga gctgatgcgt caaccattct ttttgtctcg tctgctctga
     2041 agtggtgaag tggtttactg aatttggcca cctgaacaga ggtgatatgc tcacctcaga
     2101 acaacacagg tgctccaatg aaaaaaagaa attttagcgc agagtttaaa cgcgaatccg
     2161 ctcaactggt tgttgaccag acatacacgg tggcagatgc cgccaaagct atggatgttg
     2221 gcctttccac aatgacaaga tgggtcaaac aactgcgtga tgagcgtcag ggcaaaacac
     2281 caaaagcctc tccgataaca ccagaacaaa tcgaaatacg taagctgagg aaaaagctac
     2341 aacgcattga aatggagaat gaaatattaa aaaaggctac aaccgcgctc ttgatgtcag
     2401 actccctgaa cagttctcga taatcaggaa actcagagcg cattatcctg tggtcacact
     2461 ctgccatgtg ttcggggttc atcgcagcag ctacagatac tggaaaaacc gtcctgaaaa
     2521 accagacggc agacgggctg tattacgcag ccaggtactt gaactgcatg gcatcagcca
     2581 cggctctgcc ggagcaagaa gcatcgccac aatggcaacc cggagaggct accagatggg
     2641 gcactggctt gctggcagac tcatgaaaga gctggggctg gtcagttgcc agcagccgac
     2701 tcaccggtat aagcgtggcg gtcatgagca cgttgctatc ccgaatcatc ttgagcgaca
     2761 gttcgccgta acggaaccaa atcaggtgtg gtgcggtgat gtgacctata tctggacggg
     2821 taagcgctgg gcgtacctcg ccgttgttct cgacctgttc gcaagaaaac cagtgggctg
     2881 ggccatgtcg ttctcgccgg acagcaggct taccatgaaa gcactggaaa tggcatggga
     2941 aacccgtggt aagcccgtcg gggtgatgtt ccaggcgatc aaggcagtca ttatacgagc
     3001 aggcagttcc ggcagttact gtggcgatac cggatcaggc agagtatgag tcggcgtgga
     3061 aactgctggg ataacagccc aatggagcgc ttcttcagga gtctgaagaa cgaatgggtg
     3121 ccagcgacgg gctatgtaag cttcagcgat gcagctcacg caataacgga ctatatcgtt
     3181 ggatattaca gcgcactaag accgcacgaa tataatggtg ggttaccacc aaacgaatca
     3241 gaaaaccgat actggaaaaa ctctaacgcg gaggccagtt ttagttgacc acaacagact
     3301 acctgaaggg agccgcggtc gcctagcagt tgcagtagca ggagatcatc cagccgcagt
     3361 acaggtcacg atgactctgg ttaatgatac cggctttgac cccgtatttt ccggctctat
     3421 cgctgaatca tggcgtcagc agccgtgcac accatcctat tgttgtgact gggaggctgc
     3481 caccatgctt cgcgctttcc ctctggcgaa aaagggagaa ggacgggccc gtctgccttc
     3541 actttatgcc agcttcggta agctgggtga gacaccgact cataaagata tcattgataa
     3601 caatcgatcc atcaactggc ctgtataacg tggctgccgg tgattaagaa agctgcacct
     3661 acctaagtag tagcaaacgc acacttttta gaaaaatcga tggtcagaaa ctggattagc
     3721 aattccgttc cagggttgct tttgatttac gttggcgtct gatcattgat ttatcctcaa
     3781 aagcccaacc tcattggtaa tgaaccagct ccgtgaatgt ccgctctggc acagagcgaa
     3841 attttttgat ctccccccct gaaatctaaa cttagtcatg tcacgttttt gggtttctaa
     3901 aattttaact tcgcgttttt cgttgccgta agggttatac agaaatgtcc gttaagcaga
     3961 gttcaaaatt gattgccgtg atcacgactg gtttgaaagc cgcgcccaag cctgtacagc
     4021 tctggtttgc gttgattatg aacctgtcag cctaaagcaa gcggatggac gatgagtatt
     4081 ggtaatcttt cagagtccgg aaaagttcag ccccagtctg aacaggcttg ctggcgccag
     4141 tccagtttca ttcagtcgtg gtttggttct tacggcctgt gcaatctacc tcattaggca
     4201 catcggcctg ccagataccg gctcggggtg tatttccgct tccacgctga atactgttct
     4261 cagcaatcct ggggtcatca cctcttctgg tgtgccttgt gccataacat gtccgtttgc
     4321 cattaccacc agttgatcgc agtaccggct agcctgatta aggtcgtgca gcacagcgac
     4381 caccgttttc ccctgagtcc ggagttcgcc catcaaccgc atcaggtcca cctggtgatt
     4441 gatatcaaga taggtggttg gctcatcaag taatacaacg ggcgtattct gggccaggac
     4501 catcgccaga aatgcgcgct ggcgctgacc gccggaaagc tcggttaacc gacgaacggc
     4561 aagatgattg atccgggtct ggttcatggc gacattaact cgtgcattgt cttcagcgga
     4621 gagacgcccc cagagtgaca gccagggatt acgaccatac gaaaccagct cctggactgt
     4681 gatcccctct ggcgttaaat ggtgctgagg cagcagcgaa agcctgcggg ccaactggcg
     4741 cgatgagagc atatttatgg gattatcgcc gagaaatacg gtgccagact gcggcattaa
     4801 aagccgcgaa aaacagttta acagcgtcga tttcccgcaa ccgttaggac cgatcagggc
     4861 ggtgatcttc cccgttggca gtgagagtga aacgtcgtta agtaccttgt ctgtcccgta
     4921 actgaccgtc agattttcag ttcgtaaagt catttatcgc attctcacaa gcaaccagac
     4981 aaaccacggc gcaccgataa tggcggtcag cacgccaacc gggagctcca gtgggggatg
     5041 aataattctc gccagcagat cggcaaccac caacagcaac gcacctgtca gggctgaaac
     5101 aggcagcagt ctgcggtgac gtccaccggt gatgctacgc atcatatgcg gcaccacgag
     5161 accaataaag ctaatcgggc cgcaggcggc cacgccggta gatgtcatgg cgacagctag
     5221 taacaaagcc cagaatcggg tatggggcac cgacacaccg agcgtggtgg cgcgcgcatc
     5281 gccgagtgca aggaggtcga gatcgcggca aaaactcagg ctcagcggca gaaataaaat
     5341 catcagcggg atggcaatct tcacaaagct ccagtcacgg ccccataagc tgccggtcag
     5401 ccacagcagg gcgttgttca catcctgtgg gcgcgagagc atcagataat ccgtcaggct
     5461 ggcccagcat gcagaaagcg ccacgccggt gagcgccagc ttcatcggct ggtgggtctt
     5521 tgccagcatc ttcagtaata tcaaccccgc catgccgccc gcaaaggcca gcagcggcag
     5581 caccatcacg ggcagtgacg gcataagaag tagagccccc acagaggcca ggctggcggc
     5641 atggttaacg ccgagaatat ccggtgatgc cagagggttg cgcacaatcc cctgtatcag
     5701 cacgcccgcc acggcgaggg ctgcaccgac aaacagtgcc agcagcaagc gcggcagtcg
     5761 gtactccatc aatacataat aatgctcgcg tccggcctgc cagtcggtca gcagcgcgcg
     5821 ccacggcacg gggatcactc ccatatggag tgataacagc gcacagcccg ccagggcaag
     5881 ggtgatgaaa ataaccagcg caattttcat cctcgcctcc tcacaagcca gacaaagcaa
     5941 gggctgccaa tcagcgccag cactgcgcct gcgggcagat ctccggggaa ggccagcgcg
     6001 cgtgcgagta catctgccag cagcatcagc gtggccccca gcagcatgct cactggcagt
     6061 acgttgcgct gatcgaagcc tgcccagaag cgcgccagat gtggcaccag cagaccgata
     6121 aacgccaccg gacctgccac gctgacgcac gcaccaacca gaagcagcac taacatattg
     6181 atgaccaaac gtagcctcgt caggttcact cccagcgtat gggcggtgct gtcgctgagg
     6241 ttgagcaggt tcagttgatt cgccagcagc aacacgacag ggactgcagt gaccaccacc
     6301 ggcaagagct gccagacatc ctgccagcgg gcgtgggaca ctccgcctgc cagccagtaa
     6361 aagatgccgt aagcatgatc ttcggccagc agcagggtga tgcgggtaag gcccatacaa
     6421 aaggccgaca gcgcgatact cgcgaggatc agtttgtttc tgtcatgggt atgacgaaat
     6481 ccgcctcctg cggtcatgac cagcagccag ctcacgccgc ccccgcatgc cgcgatgaac
     6541 gacagagaat agcctgcaat cggcgtcgga ctcagcgcgc tggtaagcgc catagccagc
     6601 gccgcgccgc tgttaatgcc gagcagtgaa ggagaggcca ttgggttgtg ggtcagggtt
     6661 tgcagcagcg tgcccgcgag cgccaggctt gcgccgatca gaacggcgac caggcttcgt
     6721 ggcaaacgaa ggttttgcac cagcgcttct ggtagcgttg gcgtgtgtcc aggcagcagg
     6781 gcgcgggttg catctgctcc ggaaacagga atggccgagt agcaaaacag actcagccag
     6841 aaaataataa taagtgctgc aacgggaagc ccccacagca gcaccgggtg ttttatcgcg
     6901 gtcatttcac aacggtaagc ggctgatggt ggaagatttt taccgtgtca gcggcaatac
     6961 gctctgcagc aaaaataccg cgcatccgcg cccaggtgtt actgtcgacc gaagcaacct
     7021 gctgcttctg cgcggcggtt aacatctgcc agagcggatc ttgttgccag cgtttaacaa
     7081 tgctctcttc gcgatagtgg gcaaccagca gccaggcagg attgaccgcc agcagttgct
     7141 ccaggccgat ggacggcatg gacgcacccg ccatcgcagc gggaacgttc agccccagag
     7201 aggccagcac gctgccggtc caggtctcct gagtatgcag gttgaattgc tgttcgcgtg
     7261 atgtgccaaa ggccacgcgt gtccctttgg gaagctggct ggcccactgc gccatcctct
     7321 ctttatgttg ttccaggcgt gcctgcatct ctcgcttttt acccaccatt tcgccgatga
     7381 tagccgcaga ttgcaaattt tcagcgtagg tttcgttgcg ggacttaagc agcagtaccg
     7441 gcgcgatttg ctgcaaggcg atgtaaaccc ccgcatggcg actgctgtcg gcaatgatca
     7501 ggtctggttt cagagcggca atggcttcca ggctcggctg cgcgcgcgtt ccgacggact
     7561 gccacggttt caggtgcgca cgcacttcgg gcaggatgcg ttttgcatcg ttatcgtcgg
     7621 caataccgat cgggctgacg tccacggcgg ccagcgcatc ggcgaacgag agttccagca
     7681 ccacaatccg ttgtggcgtt ttttcgagtg taaacgtgcc gtgttcgtcc tgaaccgtgg
     7741 cggcaaaggc gtggctgatc accagcagca ggcctgcaaa aacaaaacgg ataaatgcca
     7801 acataatcac attccagcta aaagcccggc aagccgggcg ttaacacatc agaacttcaa
     7861 cgacccctgc atatacagcg tgcgcggctg gcctgcatag atgcctttgt tgttgtcgtc
     7921 ataagagcgg atgaagtagt cctggtcgaa gatgtttttc acaccgaatg ccaggttcag
     7981 atctgccatc tgcgggccaa aatcatacgc cacgcgtgcg ccccagagca tgaagccggg
     8041 aatgcggccg gtactgccgt cggcgctctc tttcaccgta ttggcgttat ccgcaaactg
     8101 gctggactgg aaatcgctgt tcagattgaa cgtccagttt cctggcttgt agtccacgcc
     8161 cagcgtgcct ttatgtttcg gggagaatgg taccagattg ccgtaggtgt cgcctttctc
     8221 gcggatttcc gcgttcacat acgcatagct ggcgtagatg gaaacgttat caagcgttgg
     8281 cgttagcgta cccagatcgt aacgtgcctg cgtttccagc ccggtatggc gcgttttgcc
     8341 acgtgcagtg acggtgtcgt tggtctggtt ggagtcgtac tgattgttaa agttaatcag
     8401 gaacagcccc atttccgccg tcagcgcgcc gtcgtcgtag cgggtaccga gttcccaggt
     8461 tcgcgctttt tccggttcca cattgccgct ttgcacagcc ttgccaatct ggctgtactg
     8521 tacggtgccg aacgagcctt cagtgtttgc ataaagattc cagctgtcag tcaggtgata
     8581 gagcacgttc aacgccggaa gcggtgcgtt atagctcact tcttcgtgcg tgcctgtgat
     8641 ggcgttgttc tggtatgact cgatatgttc gaaacgcata cccggcgtga tggtccagtt
     8701 gccgatgtcg attttgtcat ccagatacca ggcgtgcgcc tcggtgccgg aacgcgtatc
     8761 gcggtcgtaa gggcttgagc cggacggcaa ctgcccgctg ctggtggcgg tgtagtaacg
     8821 catttcatgc gttgattcat tcaaatagcg atagcccacg cccacttcgt gcgcggaagg
     8881 gccgatcata aagatctggc tgtagcgtgg ctcaataccg cgcacccagt agttacgcgg
     8941 cgagagggtg atgcgtttgc cttgctccag gtagccgctg cgcagggttt gggtgtagaa
     9001 cccctgaatg ttgaatttat gctggctgtc tggctggaac tggtagccca ggctcgccag
     9061 cttgcgacga ccccagaagc ggtcatacgg gcgggtggat tgccagcgat cggcgtcgta
     9121 atccgcgcga gacaggccac cgggcatgtc ggcttcacca tcgtaatatt gcagcaggct
     9181 gttgaaggtg tgcacctcat ccggcgcata tttgcttttc agcatcaggt cgtcgatgcg
     9241 ggtggcgctg tgctcgcgcc agtcactgcc gcgcgtgccg gagtagagca gcgcggtgcc
     9301 aaaaccgttg tccgctgtgc cgcccaccat caggttgtgc gtctctttcg ggttgttttg
     9361 tgaagaggtt gggctgagct gaccttccac gcccgcctcg ataccaaagt cctgcggaat
     9421 ggcacgggta acaaagttca ccacgccgcc cacgctctgc ggtccgtaac gcaccgcacc
     9481 accaccgcgt accacgtcaa tggcatccat gttgccgagc gaaacgggag ccagtgaaag
     9541 ctgcggctga ccgtaagggg cgaaggggac ggggatgccg tccatcagga cggtcgagcg
     9601 gctggcgagg cgcgggctca ggccccggat gccaaagttc atcgccaggt cgtggctgcc
     9661 ggtgccgttg ttttccggcg cgctgacgcc agggatgcgg ttaagtacct cacgcatggt
     9721 ggttgcgccg gttttggcga aatcctcacg gcggatcacg tcacgcgcgc cagcatgttc
     9781 aaatacgtcg ttttcacgcg catcacccag ccagtcgccg accacggtca gggcatcttc
     9841 ttttggtgcg ggcgcgggct ccagcgtcca gctgttattt cccagcggtt ttacctgcag
     9901 tccgctgccg tccagcagtt gttgcaggcc gctctcgacg tcgtaatcgc cgtgcaggcc
     9961 gttgctctgc ttgccgcgcg tcaggctggc gtcaaccgag agggtaaatc cgctgtgtgc
    10021 ggcatactga ttgagcgctt tatcgagcga tcccggtgcg atattaacct gtgcagcaaa
    10081 agcggaaaac gagagaccgg ccagcggcag caggctcagg cgaatggcgt taaccaaagg
    10141 tgttgtttta cgaaaaacgc gtaacggcgt cataccttcc ccatcatcat ttttgttgtg
    10201 ttcagctatg agtcgaacga gaagggtaaa aaggacaatc gaaataagaa ttattttcct
    10261 tacagtggtg aaatgtttat ccagtaccgc gtaatagact gaattttaac gggaagcgtt
    10321 tgcgcgataa cgttcaggat cgcatcggta tttttcagcg ggaacgtccc gctcaggcgc
    10381 agcccggcaa cggcgggatc gcagcgcagc acgccgttgc ggtaacgggt tagcgtggct
    10441 atcacctcac ccagcggttt atcgctgaag ctcaggatgt ccttcgtcca gcttgtactc
    10501 tcgtcatcca gcggtttcac tgcgccaaac tcagaggcgc tgaactgcag gctttcacca
    10561 gcgttcacga tgcgtttttg cgcgggggca ctggcgagaa gcacttccac agcgtgctgc
    10621 tgcacgtcaa gctgcgtgaa attatcctgc tggcggacgg taaattctgt ccctaaagca
    10681 gtgagctggc cctgacgggt caggacacgg aaggggcgtt gcagggcatc tttcgcggta
    10741 gtaatggcga tttcaccgta ccagagccgg acggtgcgct gatgcgcatc aaaacgcaca
    10801 tccgccgcgc tttgggtatt cagcgtgagc agggagccat cttccagttg ctgacggctg
    10861 actgtgcctt tggcggtgcg gtaatctgcc cgcagacctt cgccggtttc cgactgccag
    10921 agctgccagc ctccgccagc gccgagcaac agcagtaatc ctttcatcac gtgacggcgg
    10981 gtgaggcggg tatcgtgcaa cgccctgctg gctacgtcgc caggcacacc gccaagctgg
    11041 ttgcgcaggt tttcaacctg ctgccaggcc cactggttat cctgatcctg ttcataccac
    11101 tgttgccagc gcgcttcctg ttgtgggctg acgcgctcgc cgcttagcac ggcataccag
    11161 tgggaagctg aacgcagcgc ctgacggcgg gaatcggtta acaaaggatt cataacccat
    11221 actccagacg gaacagcagg cagtgctcga cggctttcgc cacgtatttt ttcacggagc
    11281 tgatggaaac accgagtttg tgcgcaatct cgctgtatgt cagaccatcc agttgcgaaa
    11341 gcagaaacgc ttcacgtgtt ttgccgttta gcccgtccag catgctgtcg aggagttgta
    11401 gggtctcgag ttggctttcg cgttcctcag gtgaaggcgc tcccccctcc ggcataagcg
    11461 ccagcatctc cagatacgct ttttccagcg cgtttcggcg aaacaggtcc accatcacgc
    11521 gtttggcgat agtgcagagg aaggagcgag gatcgcggat cgtcgagagc gtttcgctga
    11581 ccattacccg caaaaaagtg tcctgggcaa tgtcatctgc atcaaaagca gactggagtt
    11641 tgcgcgtcag ccagcttttc aaccagccgt gatgtgtgcc ataaagcgac tcgaacgtta
    11701 aggaagctgt ggtagtggcg cggtcagaca tgcggagtgc atcaaaagtt aattatcacg
    11761 tagtcatatt aatatgagaa tggttatcat tacaattgga aataaaattg tttccaatag
    11821 acatttttaa catgttgttt ttctaagtgt tataaggtag gtataaaatg ggatggagcc
    11881 tctgcttctg gcatgtgtcg gtcagaatga ctcatgatgt ggtctgctat tattgacatc
    11941 ctcactgccc taaaggatgg ggatttcggt aatgctgcca acttactgat ttagtgtatg
    12001 atggtgattt taaggtgctt gcgtggcttc catttccatc agatgtcctt cctgctccgc
    12061 tactgaaggc gtggtgcgta acggcaaaag cactgccgga catcagcgct atctctgctc
    12121 tccttgccgt aaaacatggc aactacagtt cacttacacc gcctctcagc ccggtaagca
    12181 ccagaaaatc attgatatgg ccatgaatgg cgtcggatgt cgcgccagtg cacgcattat
    12241 gggcgttggc ctcaacacgg ttttacgtca cttaaaaaaa tcaggccgca gtcggtaacc
    12301 tcgcgcatac aaccgggcag tgatgtgatt gtctgcgctg aaatggacga acagtggggc
    12361 tacgtcggtg ctaaatcacg tcagcgctgg ctgttttacg cgtatgacag gatacggagg
    12421 acggttgtgg cgcacgtctt cggtgaacgc actctggcca cactggagcg tcttctgagc
    12481 ctgctgtcgg cctttgaggt cgtggtatag atgacggatg gctggccgct gtatgaatca
    12541 cgcctgaagg gaaagctgca cgttatcagc aagcgttaca ctcagcgcat tgagcgacat
    12601 aatctgaatc tgagacaaca tctggcaagg ctgggacgga agttactgtc gttctcaaaa
    12661 tcggtggagt tgcatgacaa ggtcatcggg cattatctga acataaaaca ctatcagtaa
    12721 gttggagtca ttacctcctc ccgaactcaa agctatcata cagataatta ctggttaacc
    12781 ctatataggg cattttaaag tgttgtgatc ttacccagca atagtggaca cgcggctaag
    12841 tgagtaaact ctcagtcaga ggtgattcac aagacaaaac agtttcaacc agtaaaaaaa
    12901 cgtaaacacc attcgcatga atttcgtagt taagatctga agcttgcaga atgcatcggt
    12961 gttactgccg gagttcgtga acttagtctg catgaatcac cacagcttta catctgtcgc
    13021 agtaaacagc aaaatcagca aacttactta attatatcga gtgcgattac aatcgctggc
    13081 gatggtacag ttggtgtggc ggtctcagtc cggaacaatt tgaaaatcag aatctcactt
    13141 aaaacggcat ccattttaca aaagatggcc gtctgtgtat ttgtaatgaa taccttctac
    13201 atgtttagag tgcataatcc acgacaaggc gatatcctaa aaattagcta gcaggcaact
    13261 ttgatggcat agataatgtt tttagtaaaa aagagttgac tgaatataat acagcctctt
    13321 cctacataag aaggaatgtg ctcacaaaaa aaaaccatta gtctg
//
LOCUS       DAZGJW010000125        13315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZGJW010000125 DAZGJW010000000
VERSION     DAZGJW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.125"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..442
                     /locus_tag="ACHKIK_003742"
     CDS             <1..442
                     /locus_tag="ACHKIK_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO9084830.1"
                     /translation="EHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSN
                     MANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPID
                     SDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(596..2245)
                     /gene="yjcE"
                     /locus_tag="ACHKIK_003743"
     CDS             complement(596..2245)
                     /gene="yjcE"
                     /locus_tag="ACHKIK_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO9084831.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2397..3746)
                     /gene="ghxP"
                     /locus_tag="ACHKIK_003744"
                     /gene_synonym="yjcD"
     CDS             complement(2397..3746)
                     /gene="ghxP"
                     /locus_tag="ACHKIK_003744"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO9084832.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4292..4756)
                     /gene="soxR"
                     /locus_tag="ACHKIK_003745"
     CDS             complement(4292..4756)
                     /gene="soxR"
                     /locus_tag="ACHKIK_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO9084833.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4842..5165
                     /gene="soxS"
                     /locus_tag="ACHKIK_003746"
     CDS             4842..5165
                     /gene="soxS"
                     /locus_tag="ACHKIK_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO9084834.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5168..6754)
                     /gene="pdeC"
                     /locus_tag="ACHKIK_003747"
     CDS             complement(5168..6754)
                     /gene="pdeC"
                     /locus_tag="ACHKIK_003747"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO9084835.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGCFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7184..7465
                     /gene="yjcB"
                     /locus_tag="ACHKIK_003748"
     CDS             7184..7465
                     /gene="yjcB"
                     /locus_tag="ACHKIK_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO9084836.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIELVMIEEMNQTPPQWPMILT"
     gene            complement(7564..8100)
                     /gene="ssb1"
                     /locus_tag="ACHKIK_003749"
     CDS             complement(7564..8100)
                     /gene="ssb1"
                     /locus_tag="ACHKIK_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO9084837.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8354..11176
                     /gene="uvrA"
                     /locus_tag="ACHKIK_003750"
     CDS             8354..11176
                     /gene="uvrA"
                     /locus_tag="ACHKIK_003750"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO9084838.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11211..11567)
                     /gene="yjbR"
                     /locus_tag="ACHKIK_003751"
     CDS             complement(11211..11567)
                     /gene="yjbR"
                     /locus_tag="ACHKIK_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO9084839.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11571..11987)
                     /gene="yjbQ"
                     /locus_tag="ACHKIK_003752"
     CDS             complement(11571..11987)
                     /gene="yjbQ"
                     /locus_tag="ACHKIK_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO9084840.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12098..12811)
                     /gene="aphA"
                     /locus_tag="ACHKIK_003753"
     CDS             complement(12098..12811)
                     /gene="aphA"
                     /locus_tag="ACHKIK_003753"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO9084841.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKLGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            13213..>13315
                     /locus_tag="ACHKIK_003754"
     CDS             13213..>13315
                     /locus_tag="ACHKIK_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004019643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084842.1"
                     /translation="MNISYVNSNKTTSLPVELDALNNKDISYAKDFSY"
BASE COUNT         3471 a         3468 c         3242 g         3134 t
ORIGIN      
        1 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
       61 aattgatagc aaactctttt gtacgtggct aacaaataca ttcctcgaaa tggttatatg
      121 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
      181 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
      241 tgatgaggct agcgtccagg gaatgggcat ttctattctc cgtggggagg aaaatatccc
      301 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
      361 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
      421 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
      481 gagagataac ttatttgttt tagaatcatg aatgttagcc cgagccaacc tgttaaaggt
      541 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
      601 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
      661 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
      721 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
      781 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
      841 gctggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
      901 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
      961 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
     1021 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
     1081 taccatccgg caagagcagc gggatagaga gcacaccggc cagagtgatc gccccacgca
     1141 ccccggcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
     1201 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
     1261 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
     1321 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagctgc agacctaaca
     1381 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
     1441 ggcgcattgc cagcggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
     1501 caaccgcagc gaggatgccg gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
     1561 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
     1621 atcgcagcga gcgaccatac agccagctca ccacaaagcc ggcgagaata ccgccaatgg
     1681 ctactttcat aaattcgacc gtcgcaccgc caacggtgaa gatcatcgtc cccatcgcca
     1741 ctgccacggc aaacttcaac gacaccaggc cggaggcgtc attcatcaac gcttcgccct
     1801 gcaaaatacc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
     1861 cagcatcggt cggagaaagc actgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
     1921 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
     1981 cgaggccaaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
     2041 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
     2101 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
     2161 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
     2221 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt tatgatctga
     2281 gaaagacgtt atgcctgaat tctcattttt tcagaatatc gcaaaagctc acacaatgtg
     2341 cggagaaaat aaaaacgggc agccattgcc acccgttttc agaatccgat gaaagattag
     2401 atagcccagc cacccgcata gaaggtcacc agcgccacgg cgataacgac cgtaccgatg
     2461 ttcaacttgc gccattcgcc ggaaaccaga cgaccaatca ccagagtcgc gaagccgatc
     2521 atgatgcctg ttacgatgtt acaggtcagc acgatgaata ccgccgtaac cagacccgcc
     2581 atcgcatcaa caaaatcagc aaagtcgatt ttcgccacgt tgctcagcat cagcaggcca
     2641 acgtacatca gcgccggagc cgttgcatac cccggaacga ggtaagagag cggagagagg
     2701 aacagaatca gcaggaacag cacgccaacg gtgatagccg tcaaaccggt tttaccgccc
     2761 gccgccgtac ccgccgcaga ctcgatgtat accgctgccg gagcagcacc caccaggcca
     2821 gagaaaacgc tgctcatgga gtcagtggtc agtgctttcc caccgtcgat gatctgccca
     2881 tctttatcca gcaggttcgc ctggccggcg acggcacgga tagttccggt ggcatcaaat
     2941 actgccgtca tcaccagcgc cagaacgctt ggcaggacta caggattcag cgcgcccata
     3001 atgtccaggc tgccaatcag tgaattgccg ttttcatcgc tcaatgaagg catggcgaaa
     3061 acgccggaga aatggacgtt aggatcgaag atcaaaccga caattgagat accgataatg
     3121 gtcagcagaa tgccaccagg gactttcagt ttttccaggc cgatgatcac cgccagacct
     3181 actagtgaca taatcaccgg gaaggtcgcg aaatcaccca gcgcaacggg cagaccatca
     3241 agcgggtttt taatcaccag accgacacca ttagcggcaa tgagcagcag gaacagaccg
     3301 ataccgatcc ccgtgccgtg cgccacaccg tgcggcaagt tgcgcaaaat ccagctacgg
     3361 atacccgtgg cagaaattac cgtaaacagc acccccatca ggaacacggc acccagcgcg
     3421 acaggtacgc taatatgttg ccccagcacc aggctgaatg cggtaaacgc cgtcagggag
     3481 atggcgcaac caatcgccaa cggcagatta gcccacagac ccatcacgat agaaccgagt
     3541 ccggcaacca gacaggttgc aacgaaaact gccgcaggcg ggaagcctgc tttacccaac
     3601 atacctggaa cgacgatgac cgagtagacc atcgccagaa acgttgttaa cccggcaacc
     3661 acttcctgac ggacagtgct tccacgttgt gaaattttaa accaggcgtc gagtgaaccg
     3721 ccggtacgcg ctgatggcgt agacataaga aaacatcccc tgagagttta attttcgtca
     3781 agatgcgtgg cggacaatcc cctgccagcg aaacgttatc ttcctgctcc aggtttgcga
     3841 caatttactg cgtcgcaaaa aagcaaacgt ttagctccgc gcatacaaaa cgggtggtaa
     3901 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
     3961 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
     4021 gtgcgcaaga ttgcagcgtt atcgagccgt ataccgttca ggattgcgat aaaaaatcgt
     4081 ctgagatgac aaaaatattg cggataaaaa gtatactttt acactggcga agaaatgccg
     4141 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatggggt
     4201 gtgttgacgt cgggggaaac cctcctgtgt accagcggga tagagagaaa gacaaagacc
     4261 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg ttcatcttcc agcaagcgtg
     4321 cgccggtacc ttcttctcct aagcggtcgc ccgggttacg caacgggcaa tcactgcgcg
     4381 aaaggcagcc acaaccaata catccgtcca gttcgtcacg cagcgctact aaggtatgaa
     4441 tgcgccgatc caactcttct cgccattggg acgaaagctg tttccactct ttcgcactta
     4501 acgtatgccc ttcgggcaac acgccaaacg cttcaccaat ggtcgccagc ggaatgccaa
     4561 tacgctgagc aattttgata attgcaacat atcgcaacac atcacgttta tatcgccgct
     4621 gattgccgct gttacggata ctggtaatca accctttact ttcatagaaa tgcagcgccg
     4681 ataccgccac accgctgcgt ttcgccactt cgccgggggt tagcagcgct ttaatgcggg
     4741 gtaatttctt ttccataaat cgctttacct caagttaact tgaggaatta tactccccaa
     4801 cagatgaatt aacgaactga acactgaaaa gaggcagatt tatgtcccat cagaaaatta
     4861 ttcaggatct tatcgcatgg attgacgagc atattgacca gccgcttaac attgatgtag
     4921 tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg aatgttccgc acggtgacgc
     4981 atcagacgct tggcgattac attcgccaac gtcgcctgtt actggccgcc gttgagttgc
     5041 gcaccaccga gcgtccgatt tttgatatcg caatggacct gggttatgtc tcgcagcaga
     5101 ccttctcccg cgttttccgt cggcagtttg atcgcactcc cagcgattat cgccaccgcc
     5161 tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa ttgctccatc cattgcataa
     5221 acacctgcgg cggcatcgcc ttcgcaaaaa aacatccctg gcaatagcgc acgccgcgtt
     5281 tgcgcagcca gttaacctgc tcctcagttt cgacgccttc agcgatcgtt tttaacccca
     5341 ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg actggttttg tgggtggtca
     5401 gcgtttcaac aaacgattta tcgattttca aaatatcgac attcaatgat ttaaggttat
     5461 gcaagttaga gtagccaata ccaaaatcat caattgccac ttcgtaacca gcctggcgga
     5521 aagccagaat aatcggcgtc attttgtcaa catcaagaaa tgcatgttca gtcacttcaa
     5581 atttaatttg ctgcggacgc accgcgtatt gctctgtttt ctgattgatt cgcgctatca
     5641 accgtgacgt atgaaaatcg gaggccgaca ggttaataga aacatagcga tcggcatgtg
     5701 ttgccaagta cgcgcccaga tcacggaaga cattatcaat aacataatca gttatctgtt
     5761 ctatcatccc ctccttttct gccagcggaa taaactctgc cggattcatt atttgcccct
     5821 gctcaccaag ccaacgtaac aacgcttcag cgccgataca tttttctgtt ttgatatcga
     5881 ttattggctg gtaataaaga caaagttgat gtttttcgag ggcgcgttgc aatttacgtt
     5941 tgggagataa atagttttgt cgaatacgta gccagagtaa tagcagaaca agactcccca
     6001 aaataccggc gggcaacgca aaaataagat gattataaaa atgggttata agacgttgat
     6061 atgaagtcgc aacaatggct gcaattgggc gttttgtcga ataaactgtc gcatataaat
     6121 agccatttct ttgtacggtt aaatccttca aatgaatcag cggagaaaac gttgctgccg
     6181 aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt atcatacaca ccccattgca
     6241 atgtcggatc atcagacatc acttcactcc agaagagagg gttgataacc gccacataat
     6301 ttccccgctg catataggtc attttatagc cagagaaaaa aggcgtatcg cggtaataat
     6361 agatagaaac gttaggttca cgcttataat cggccggtgc aatcgtatag ccgtttacag
     6421 gcgctatcag cgatgagcat aaaaaatggt tatcacgggc atagatcaat tcattaatat
     6481 aaagatagcc acgaataata ttcaacattc gcttttgatg ggctggagtg caaacttgcc
     6541 cctgatagcg ttcagctgca tcgctcacta aatctgcctg gcgaatgacc agctcggatt
     6601 tatccagtgc cagttgagca aaggttcgga gctgattatt cacttctgat tttgcccaaa
     6661 ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac taaaaagata atgcccggca
     6721 acgccagtaa ttggtgtcgt gcacgatgac tcattgctat caatttcctg cctgtttata
     6781 cccggccatt gcaaggtgaa aaccctgggt accgacgtct ttagataaat cttacggtat
     6841 aaatagcact tttgcctgat ttgaaattga tacctttcta atcgtcacct ggatgaaaat
     6901 aattcattga tattcaaaat taatttccta caggaatgca gaggcggcgg gaagatatgc
     6961 ccttaatgcc actgaaaaac cacataatct gtacagcaat aaaagtcacg gcctaatatg
     7021 gctaacaaac aatcaattgt gatatagttc acaaaattaa tgaaacaaac agagtgtttc
     7081 atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac cacgcctccc gtgaacaacg
     7141 ccgcgctgtc cagggcatcg gctcttttca gaggaattgt ttgatggcta ccctcaccac
     7201 tggcgtggtt cttcttcgct ggcaacttct tagtgccgta atgatgtttc tggccagcac
     7261 actcaacatc cgttttcgtc ggtctgatta tgtcgggctt gcggtgatca gcagcggtct
     7321 gggcgttgtt tccgcctgtt ggttcgcaat ggggttgctt ggcatcacaa tggcggacat
     7381 caccgctatc tggcacaaca tcgagttggt gatgatagaa gagatgaatc agacaccgcc
     7441 acaatggcca atgattttga cctgatacgt agaagcctcc ataacggagg cttctttttt
     7501 atggctggca gatgctttaa tcatccacct taaaacaata taacctattg ttttaatgac
     7561 aaatcagaac ggaatgtcat catcaaagtc catcggcggc tcgttagacg gcgctgccgg
     7621 agcggactgc tgcgggcgag actgcgcgcc gccgctgaac tgattgccac cctgcggctg
     7681 ctgtggctga ccccaaccgc cctgcggctg accaccaccg atattgccac ctgccggagc
     7741 gccaccaccc tgacgaccac ccagcatctg catggtgccg ccaacgttca ccacgacttc
     7801 tgtggtgtag cgatcctgac cggattgatc ggtccattta cgggtacgca gctgaccttc
     7861 gatataaacc tgagaacctt tacgcagata ttcgctcgcc acttctgcca gtttgccgaa
     7921 cagcacaacg cggtgccatt cagtctgttc tttcatctcg ccggtcgctt tatcacgcca
     7981 ggattcggaa gtagccagcg taatgttggc aactgcgcca ccatttggca tgtagcgtac
     8041 ttccgggtcc tgacccagat taccaacgag aataaccttg tttacgcctc tgctggccat
     8101 gttcgtgtct cctgaaaaaa atcgttctga ataagtgtaa acgcgcgatt gtaccattac
     8161 caatagcgct tttactatgt tgtgacctcg gttccgggaa acaaacctgg ccagacattg
     8221 ttacacaaca ctccgggtaa tgcattccaa tactgtatat tcattcaggt caatttgtgt
     8281 cataattaac cgtttgtgat cgccggtagc accatgccac cgggcaaaaa agcgtttaat
     8341 ccgggaaagg tgaatggata agatcgaagt tcggggcgcc cgcacccata atctcaaaaa
     8401 catcaacctc gttatccccc gcgacaagct cattgtcgtg accgggcttt cgggttctgg
     8461 caaatcctcg ctcgctttcg acaccttata tgccgaaggg cagcgccgtt acgttgaatc
     8521 cctttccgcc tacgcgcggc agtttctgtc actgatggaa aagccggacg tcgatcatat
     8581 tgaggggctt tctcctgcca tctcaattga gcagaaatcg acgtctcata acccgcgttc
     8641 tacggtgggg acaatcaccg aaatccacga ctatttgcgt ttgttgttcg cccgcgtcgg
     8701 cgaaccgcgc tgtccggacc acgacgtccc gctggcggcg caaaccgtca gccagatggt
     8761 ggataacgtg ctgtcgcagc cggaaggcaa gcgtctgatg ctgctcgcgc caatcattaa
     8821 agagcgcaaa ggcgaacaca ccaaaacgct ggagaacctg gcaagccagg gttacatccg
     8881 tgctcgtatt gatggcgaag tctgcgatct ttccgatccg ccgaaactgg aactgcaaaa
     8941 gaaacatacc attgaagtgg tggttgatcg cttcaaggtg cgtgacgatc ttacccaacg
     9001 tcttgcggag tcgtttgaaa ccgcgctgga gctttccggt ggtacagcgg tagtggcgga
     9061 tatggacgac ccgaaagcgg aagagctgct gttctccgcc aacttcgcct gcccaatttg
     9121 cggctacagt atgcgtgaac tggagccgcg actgttttcg tttaacaacc cggcaggtgc
     9181 ctgcccgacc tgtgacggcc ttggcgtaca gcaatatttc gatcctgacc gcgtgatcca
     9241 aaaccccgag ctgtcactgg ctggcggtgc gatccgtggc tgggatcgcc gcaacttcta
     9301 ttacttccag atgctgaaat cgctggcaga tcactataag ttcgacgtcg aagcgccgtg
     9361 gggcagcctg agcgcgaacg tgcataaagt ggtgttgtac ggttctggca aagaaaacat
     9421 tgaattcaaa tacatgaacg atcgtggcga tacctccatc cgtcgtcatc cgttcgaagg
     9481 cgtgctgcac aatatggagc gccgttataa agagacagaa tccagtgcgg tacgtgaaga
     9541 attagccaag tttatcagca atcgcccatg cgccagctgc gaagggacgc gtctgcgtcg
     9601 ggaagcgcgc cacgtttatg tcgagaatac gccgctgcct gctatctccg acatgagcat
     9661 cggtcatgcg atggaattct tcaacaatct caaactcgct ggtcaacggg cgaagattgc
     9721 ggaaaaaatc cttaaagaga tcggcgatcg tttgaaattc ctcgttaacg tcggcctgaa
     9781 ttacctgacg ctttcccgct cggcagaaac gctttccggc ggtgaagccc agcgtatccg
     9841 tctggcgagc cagattggtg cgggcctggt tggcgttatg tacgtgctgg acgagccatc
     9901 tatcggcctg caccagcgcg ataacgagcg cctgttgggt acgcttatcc atctgcgcga
     9961 tctcggtaat accgtgattg tggtggagca cgacgaagac gcaattcgcg ccgctgacca
    10021 tgtgatcgac attggcccgg gcgcaggtgt tcacggcggt gaagtggtcg cagaaggtcc
    10081 gctggaagcg attatggcgg tgccggagtc gttgaccggg cagtacatga gcggcaaacg
    10141 caagattgaa gtgccgaaga aacgcgttcc ggcgaatccg gaaaaagtgc tgaagctgac
    10201 aggcgcacgc ggcaacaacc tgaaggacgt gacgctgacg ctgccggtgg gtctgtttac
    10261 ctgcatcacc ggggtttcag gttccggtaa atcgacgctg attaacgaca cactgttccc
    10321 gattgcccaa cgccagttga atggggcgac catcgccgaa ccagcaccgt atcgcgatat
    10381 tcaggggctg gagcatttcg ataaagtgat cgatatcgac caaagcccaa ttggtcgtac
    10441 tccacgttct aacccggcga cctataccgg cgtgtttacg cctgtgcgcg aactgtttgc
    10501 gggcgtaccg gaatcccgtg cgcgtggtta tacgccagga cgtttcagct ttaacgtccg
    10561 tggcgggcgc tgcgaagcct gtcagggcga cggtgtgatc aaagtagaga tgcacttcct
    10621 gccggatatc tacgtgccgt gcgatcagtg taaaggtaaa cgctataacc gtgaaacgct
    10681 ggaaattaag tacaaaggca aaaccatcca cgaagtgctg gatatgacca tcgaagaggc
    10741 gcgtgagttc tttgatgccg tacctgcact ggcgcgtaag ctgcaaacgt tgatggacgt
    10801 tggcctgacg tacattcgcc tggggcagtc cgcaaccaca ctttctggtg gtgaagccca
    10861 gcgcgtgaag ctggcgcgtg agctgtcaaa acgcggcacc gggcagacgc tgtatattct
    10921 cgacgagccg accaccggtc tgcacttcgc cgatattcag caactgctcg acgtactgca
    10981 taaactgcgc gatcagggca acaccattgt ggtgattgag cacaatctcg acgtgatcaa
    11041 aaccgctgac tggattgtcg acctgggacc agaaggcggc agtggcggcg gcgaaatcct
    11101 cgtctccggt acgccagaaa ccgtcgcgga gtgcgaagct tcgcatacgg cacgcttcct
    11161 caagccgatg ctgtaatcgt taaggccgct ttctgagcgg ccttttcctt tcagagttgt
    11221 accagcaatt tacgcttttc ttccggtagt aaattcactg cctgctgata agacgcatcc
    11281 accagataat agatttgcga atccggcagc gaaccgtcga gatacacggt gctccagtgc
    11341 gctttattca gatggcggct aggacgcaca tcgctgtgct gctgacgtag cagctccgcc
    11401 agctccgggc tggttttcag cgaaacagct gggcgatttt ctacttcttt caccatcgca
    11461 aacagtacat cttccacttt gatctgcgtc gctttccagt cgttatgcac gctctgttct
    11521 gcgcctggtt ttgccatgca atattgtagc aactccgaaa tggtcatttt ttactcccct
    11581 tgtagtgtcg cgatgatgcg acgcgatccg ccgtggatgc gatgttcccc cagccagatg
    11641 ccttgccagg tgccggtctg aatacgccct ttatgtaccg gcaatacaag cgatgttccc
    11701 agcattgagg atttgatatg agaaggcata tcgtctgctc cctcatagtc atgctcataa
    11761 tttccgttgt cgggaacggt gcggaggaaa aaacgctcca tgtcgtggcg tacggtggga
    11821 tcgcagttct cattaagtgt cagagaggcg gaggtatgtt gcagcaacag atgcagtaag
    11881 ccgatgttaa cgcgcggcat atcagccagc tgattcagaa tttcatccgt taccagatga
    11941 aacccacgag atttggcgct aagcgtgagc gtcttttgat accacatgtg ctgctccttg
    12001 ataaaactct cttaatcagt ttgcagcaag acagcgaaag gataaaggtg tgattaaaaa
    12061 aacagcattc gggagagcgt cacgctctcc cgctctgtca gtattctgaa ttgacgatca
    12121 cctcttcacc aaacgcaccc gcttgtggca agggtttgta ggtagagttg gaggcgcgca
    12181 gaatgcggat accacgagcg ccgacatcgc gtgcggcggt aatatcatta tcagaatcgc
    12241 cataaaaaat tcggatattt ttatcctgca gccattgcga ttttgtattt tgccctagtt
    12301 tatcgcccgc aaagatcacc ggattcatgt tggtggcagg aatatgaaaa ttatccgcca
    12361 gcgtttttga aaccgtttct gttttcgtcg gactacgacc agtcacaaag aagatcgcgt
    12421 caccgcggcg tacatgcata tcaatcagct ggcgagcgac ctcttttgga atgctgaatt
    12481 catcccagcc attgttcatt ttttcccaga acacaggatt tttcagataa tcttcgcttt
    12541 ctggcgagaa ggtttttttg ccgcgccaga agcccggact ggaaaaaagt accgtgtcat
    12601 cgatatcaaa ccccaccgcc attggcggac gccctgcgag gctattttca atttgtgcga
    12661 ccgaaaccca atgaatgggt gcctgttcag caagcctggc aacgttagtc ccagggttaa
    12721 gcggtgaagg agatgaggcc agggcaacag cggaactgtt tagagcgaac aataagcaaa
    12781 cggcactgat tgcctgtgtg atcttgcgca tatttttccc taaatagtca gtatgttgaa
    12841 actttttatt gtgagatttg ttgcaaaaac catctgacca taacgccagc tgccggggaa
    12901 aggaaggatt atctgcggtt tttgtgatga aaagcaggat gattatattt aacccagtaa
    12961 atacgttcct gtcttttttt gtataaaaaa tgtttattac tcgatgtaag gatacattaa
    13021 ttttacgttt attatgatga atcctcggca aatgagcgaa atttgataga acatttatct
    13081 gctgtacgat ttaatgaact taatgataca gcattacaaa aacaatcgaa gtttataaag
    13141 atgatttctg attgaccagc cccctaccct acaatggtga ttaattttat tactcgcgat
    13201 ggagatgatg atatgaatat atcatatgtg aatagcaaca aaacgacatc tttacctgtt
    13261 gagctagatg cactaaataa taaagatatc tcttatgcca aagatttttc ttatg
//
LOCUS       DAZGJW010000126        13287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZGJW010000126 DAZGJW010000000
VERSION     DAZGJW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.126"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            73..786
                     /gene="ydiV"
                     /locus_tag="ACHKIK_003755"
     CDS             73..786
                     /gene="ydiV"
                     /locus_tag="ACHKIK_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO9084843.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            849..2285
                     /gene="selO"
                     /locus_tag="ACHKIK_003756"
     CDS             849..2285
                     /gene="selO"
                     /locus_tag="ACHKIK_003756"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO9084844.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSYLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(2289..2480)
                     /gene="hemP"
                     /locus_tag="ACHKIK_003757"
     CDS             complement(2289..2480)
                     /gene="hemP"
                     /locus_tag="ACHKIK_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO9084845.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2612..3658)
                     /gene="aroH"
                     /locus_tag="ACHKIK_003758"
     CDS             complement(2612..3658)
                     /gene="aroH"
                     /locus_tag="ACHKIK_003758"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO9084846.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLEMARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNSRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(3815..4648)
                     /gene="ppsR"
                     /locus_tag="ACHKIK_003759"
     CDS             complement(3815..4648)
                     /gene="ppsR"
                     /locus_tag="ACHKIK_003759"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO9084847.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKLLDIMGLSRRM
                     Y"
     gene            4981..7359
                     /gene="ppsA"
                     /locus_tag="ACHKIK_003760"
     CDS             4981..7359
                     /gene="ppsA"
                     /locus_tag="ACHKIK_003760"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO9084848.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(7416..9062)
                     /gene="fadK"
                     /locus_tag="ACHKIK_003761"
     CDS             complement(7416..9062)
                     /gene="fadK"
                     /locus_tag="ACHKIK_003761"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO9084849.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(9119..9412)
                     /locus_tag="ACHKIK_003762"
     CDS             complement(9119..9412)
                     /locus_tag="ACHKIK_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO9084850.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(9409..10698)
                     /locus_tag="ACHKIK_003763"
     CDS             complement(9409..10698)
                     /locus_tag="ACHKIK_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO9084851.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCIMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(10754..11692)
                     /locus_tag="ACHKIK_003764"
     CDS             complement(10754..11692)
                     /locus_tag="ACHKIK_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO9084852.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(11712..12476)
                     /locus_tag="ACHKIK_003765"
     CDS             complement(11712..12476)
                     /locus_tag="ACHKIK_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO9084853.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            12792..>13287
                     /locus_tag="ACHKIK_003766"
     CDS             12792..>13287
                     /locus_tag="ACHKIK_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFO9084854.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENF"
BASE COUNT         3209 a         3319 c         3426 g         3333 t
ORIGIN      
        1 aattgttatg tcttatggcc tcatcccttt tagccggata ctgaaaaaca tccttcgaga
       61 gggacggtta ccatgaagat ttttttggag aatctttatc attctgattg ctatttcctg
      121 ccgataagag ataaccagca agaccttgtc ggcgttgagt taattaccca tttctccagt
      181 gaagatggta cggttcgtat cccgacgagt cgggttatag cgcaacttac cgaggagcaa
      241 cattggcaat tattttcaga gcagttggaa ttactaaaat catgccaaca tttttttatt
      301 caacataaac tctttgcctg gcttaattta acgccacaag tggctacatt attattagag
      361 agggataatt acgcaggcga actattaaaa tatccgttta ttgaactgtt gattaatgag
      421 aattacccgc atctcaatga aggaaaagat aatcgcgatc ttttatcctt atcgcaaatg
      481 tatccccttg tgttaggcaa tttgggagca ggtaatagca caatgaaagc ggtttttgat
      541 ggtcttttca cccgcgtcat gctggataaa agttttattc agcaacaaat cacgcatcgc
      601 tccttcgaac cgtttattcg tgcgatccag gcgcaaattt ccccttgttg caattgcatc
      661 atcgcggggg gcattgatac gacagaaata ttggcacaga ttaccccctt cgatttccat
      721 gctctccagg gatgcctgtg gcctgccgtt cctataaatc agataacgac gttggttcag
      781 cgataaccct cctgtttgct ggtgtttaag acgagagtaa ccgtctacac tattaaacag
      841 gaggatctat gaccctgtct tttattaccc gctggcgcga tgaattgcca gcaacctata
      901 cagcactttc ccctacgcct ttaaataatg cccggctgat ttggcataat accgaactcg
      961 ctaacacgct gagtattcca tcgtcgctgt ttaaaaatgg cgcaggtgtc tggggcggcg
     1021 aaaccctact gcctggtatg tcaccactgg cgcaggttta cagtggtcat cagttcggcg
     1081 tctgggcggg ccaactgggt gatgggcgcg gtattttact cggcgaacaa ctgcttgctg
     1141 atggcactac aatggactgg catctgaaag gtgctggcct gacgccttat tcgcgaatgg
     1201 gtgatggacg ggcggtttta cgttcgacga tacgagaaag tctcgccagt gaggcgatgc
     1261 attatctggg cattccgacg acccgcgcgt taagtatcgt caccagcgat tcgccagtgt
     1321 atcgggaaac ggtggagcca ggcgcgatgc tgatgcgtgt ggcaccaagt catctgcgct
     1381 ttggtcattt cgaacatttt tactatcgcc gcgagccgga aaaggttcgt cagttagctg
     1441 actttgccat tcgtcattac tggtcatatc ttgaagatga tgaggacaaa taccgcctct
     1501 ggtttagcga tgttgtcgca cgtaccgcat cgttaattgc ccaatggcag acggtcggct
     1561 ttgctcatgg ggtgatgaat accgataaca tgtcgctgct ggggctgacg cttgattacg
     1621 ggccgtttgg ttttctggat gattacgaac ccggttttat ttgtaatcac tcggatcatc
     1681 aagggcgtta tagctttgat aatcaacctg ctgtcgcgtt gtggaattta cagcgtctgg
     1741 cgcagacatt gtcaccattt gttgccgtag atgccctgaa tgaggccctg gacagctatc
     1801 agcaggtttt gttgacgcat tatggacaac ggatgcggca gaaactgggc ttcatgacgg
     1861 agcaaaaaga ggataacgcg ctactgaatg aattattcag tctgatggcg cgagagcgca
     1921 gcgattatac ccgcacattc cgcatgctga gtctgaccga gcagcacagc gcggcgtcac
     1981 cgctacgtga tgagtttatt gatcgtgcgg catttgatga ctggtttgcc cgttatcggg
     2041 ggcgtttgca acaagacgag gttagcgata gtgagcgtca gcagctgatg caaagcgtta
     2101 acccagctct ggtgttgcgc aactggttgg cgcaacgggc gattgaagcg gcagaaaagg
     2161 gtgatatgac ggaattgcac cgcctgcatg aggcgttgcg aaatcctttc agcgacagag
     2221 ctgatgacta tgtcagtcgt ccacctgact ggggtaaacg gctggaagtc agctgctcga
     2281 gttaaaggct acttggtcaa cagcagcttg ccagcctgag ttttacggag cagatattct
     2341 tgcccgtcat gatcgataat cagtttgcca tccggaccta acagtgtctg gctggaaatt
     2401 cgccgaatag gctgcgggga agcggtcttg tgattggcat ccgtttcagg cgtgagtttt
     2461 ctgctatccg tataacgcat aaaaattaat aataattgat ataacaatga ttcttattat
     2521 caatattatg gctgaccaga gcaagcagta atgtgctgag aagacaacaa gttgcgcaga
     2581 aatgcccatc ccatcaggaa tgggcacgca ttcagaagcg ggtatctacc gcagaggcaa
     2641 gtttttcgac caggcgttcg gtatcttccc agcccagaca cgggtcggta atggattgac
     2701 cgtaagtgag cggctgaccg ccgacgattt tttgcgttcc ttcgcgcagg aaactttccg
     2761 ccataattcc agcaatcgcc gtagagccat tgcggatttg ctgacaaata tcctcacaaa
     2821 cttctaactg gcgacggtgc tgcttctggc agttaccgtg gctgaaatcc accaccagat
     2881 gttcaggtaa atcaaactcg tgcagcgtat cgcaggctgc ggcgatatca tcggcatgat
     2941 aattcggttt tttgccgcca cgcataataa tgtggccata cgggttgccg ctggtctgat
     3001 agatggtcat ctgaccattt ttgtctggcg agagaaacat atggctggcg cgggctgcgc
     3061 ggatagcatc cacggcaatc cgcgagttgc catcggtacc atttttaaaa cctaccggac
     3121 aggagagtgc cgaagccatt tcacggtgga tctgactttc ggtagtacgt gcgccaatcg
     3181 cgccccaact gattaaatca gcaataaact gaccggtcac catatcgagg aactcagtcg
     3241 cggtagggac acccagctca tttacctgta aaagtaattt gcgcgccatc tccagaccgt
     3301 gatttacccg atagctgccg tttaaatccg gatcggagat tagccctttc cagccgacaa
     3361 cagttcgtgg tttttcaaaa taggtgcgca ttacgatttc cagccgtgac tggtactggt
     3421 tgcgcagcga ctgcagacgg gtggcgtact ccattgcagc ggtgagatcg tggatcgagc
     3481 aggggccaat aatgaccaac agtcgcttat cttcaccatt cagtattttt tcaattctgc
     3541 ggcgggagtc ggtgacatgg gtggcgacgc caggcgttac gggataccgt agcgcgagtt
     3601 cggcgggcgt taccaggctc tcaatacgcg cagtacggag ttcgtcagtt ctgttcatta
     3661 caagtctcag aaatttgttg cttctacggc aggactaccg ggaagtgatg cgcatcacga
     3721 taaaccaatc gcagctgatt tcaagtgcga ggtgtgtcac acctcgccgg gtggttagca
     3781 tgataacaaa aaaataagct aatgcactag ttctctagta cattcggcgg ctaagcccca
     3841 tgatatcgag gagcttggtg gcaatctctt ctaccgaata attggtactg ttaatccacg
     3901 ggatctgatt tttacggtac aacgcttcta cttccgcgac ttccatcctg cactgacgaa
     3961 gcgaggcata gcggctgttc tcccgacgtt cctcgcgaat cgccgccaga cgttccgggt
     4021 cgatagtcag gccgaacaat ttatgctgaa gcggtttgag cgacgcgggt agcaccagat
     4081 tgtccatatc gtcggcaata aaggggtagt ttgccgcgcg gataccaaac tgcattgcca
     4141 gatacagact ggtgggggtt ttaccacagc gagaaacacc gagcaggatc acctgagcct
     4201 ggtccagatt gcgcaacgaa atgccgtcat cgtgggcgag ggtgtaatca atcgccgcaa
     4261 tgcgcgcatc atatttattg agattattag ggttaaggcc atgggtacga tgagcaatcg
     4321 gcgttggatc cagtttcatc tcttgttgta gcggggcaac cagcgcctga acaatatcct
     4381 ggcaaaagcc ttcactttgc aagatgatgg cgcgaatctc cggcaacacg atggagtaga
     4441 agaccagcgg gcgcacacct gtctggtgat aaattgcgtc aatctgatcc ttcactgccc
     4501 gtgcacggct ctcattttcg acaaacggca gcgtaatgct gctgatagtg acgggaaatt
     4561 gtgacattac tgcgtgtcct aatacctccg cagttattgc cgtaccatca gaaatataaa
     4621 aaacgtggcg atcaacagca ttatccattt tgttcttccc gtgatgcaga cataattcta
     4681 tgaaagcata aattaaaaac gcacagaagc gtaggacgta atgtctggtt tataaaatga
     4741 accttcaatt ttatttttta tgaaaacagc atttcatttt tatggtttcg tttataccga
     4801 tggtttatgt ggaaattgtc gaatagagca ggtttgcgca acgctgggat cagtcttaaa
     4861 aagtaaaaaa atatatttgc ttgaacgatt caccgttttt ttcatccggt taaatatgca
     4921 aagataaatg cgcagaaatg tgtttctcaa accgttcatt tatcacaaaa ggattgttcg
     4981 atgtccaaca atggctcgtc accgctggtg ctttggtata accaactcgg catgaatgat
     5041 gtagacaggg ttgggggcaa aaatgcctcc ctgggtgaaa tgattactaa cctttccgga
     5101 atgggtgttt ccgttccgaa tggtttcgcc acaaccgccg acgcgtttaa ccagtttctg
     5161 gaccaaagcg gcgtaaacca gcgcatttat gaactgctgg ataaaacgga tattgacgat
     5221 gttacccagc ttgcgaaagc gggcgcgcaa atccgccagt ggattatcga cactcccttc
     5281 cagcctgagc tggaaaatgc catccgcgaa gcttatgcac agctttccgc cgatgacgaa
     5341 aacgcctctt ttgcggtgcg ctcctccgct actgcagaag atatgccgga cgcttccttt
     5401 gccggtcagc aggaaacctt cctcaacgtt cagggttttg acgccgttct cgtggcagtg
     5461 aaacatgtat ttgcttctct gtttaacgat cgcgccatct cttatcgtgt gcaccagggt
     5521 tacgatcacc gtggcgtggc gctctccgcc ggtgttcaac ggatggtgcg ctctgacctc
     5581 gcatcttctg gcgtgatgtt ctccattgat accgaatccg gctttgacca ggtggtgttt
     5641 atcacttccg catggggcct tggtgaaatg gtcgtgcagg gcgcggttaa cccggatgag
     5701 ttttacgtcc ataaaccgac acttgcagcg aatcgcccgg ctatcgtgcg tcgcaccatg
     5761 gggtcgaaaa aaatccgcat ggtttacgcg ccgacccagg agcacggcaa gcaggttaaa
     5821 atcgaagacg taccgcagga acagcgtgac atcttctcgc tgaccaacga agaagtgcag
     5881 gaactggcaa aacaggccgt acaaattgag aaacactacg gtcgcccgat ggatattgag
     5941 tgggcgaaag atggccacac cggcaaactg ttcattgtgc aggcgcgtcc ggaaaccgtg
     6001 cgctcacgcg gtcaggtcat ggagcgttat acgctgcatt cacagggtaa gattatcgcc
     6061 gaaggccgtg ctatcggtca tcgcatcggc gcgggtccgg tgaaagtcat ccatgacatc
     6121 agcgaaatga accgcatcga acctggcgac gtgctggtca ctgacatgac cgacccggac
     6181 tgggaaccga tcatgaagaa agcatctgcc atcgtcacca accgtggcgg tcgtacctgt
     6241 cacgcggcga tcatcgctcg tgaactgggc attccggcgg tagtgggctg tggtgatgca
     6301 acagaacgga tgaaagacgg tgagaacgtc actgtttctt gtgccgaagg tgataccggt
     6361 tacgtctatg cggagttgct ggaatttagc gtgaaaagct ccagcgtaga aacgatgccg
     6421 gatctgccgt tgaaagtgat gatgaacgtc ggtaacccgg accgtgcttt cgacttcgcc
     6481 tgcctgccga acgaaggcgt gggccttgcg cgtctggaat ttatcatcaa ccgtatgatt
     6541 ggcgtccacc cacgcgcact gcttgagttt gacgatcagg aaccgcagtt gcaaaacgaa
     6601 atccgcgaga tgatgaaagg ttttgattct ccgcgtgaat tttacgttgg tcgtctgact
     6661 gaagggatcg cgacgctggg tgccgcgttt tatccgaagc gcgtcattgt ccgtctctct
     6721 gattttaaat cgaacgaata tgccaacctg gtcggtggtg agcgttacga gccagatgaa
     6781 gagaacccga tgctcggctt ccgtggcgcg ggccgctatg tttccgacag cttccgcgac
     6841 tgcttcgcgc tggagtgtga agcagtgaaa cgagtgcgca acgacatggg gctgaccaac
     6901 gttgagatca tgatcccgtt cgtgcgtacc gtagatcagg cgaaagcggt ggttgaagaa
     6961 ctggcgcgtc aggggctgaa acgtggcgag aacgggctga aaatcatcat gatgtgtgaa
     7021 attccgtcca acgccttgct ggcagagcag ttcctcgaat atttcgacgg cttctcaatt
     7081 ggctcaaacg atatgacaca gctggcgctc ggtctggatc gtgactccgg cgtggtgtct
     7141 gaattgttcg atgagcgcaa cgatgcggtg aaagcattgc tgtcgatggc tatccgtgcc
     7201 gcgaagaaac agggcaaata tgtcgggatt tgcggtcagg gtccgtccga ccatgaagac
     7261 tttgccgcat ggttgatgga agaggggatc gatagcctgt ctctgaaccc ggacaccgtg
     7321 gtgcaaacct ggttaagcct ggctgaactg aagaaataaa ataaatcccc ggcggcgttc
     7381 agtcgccggg tttatgtgat ccccgaagat aaaacttatt caatctcttc acagacatcc
     7441 tgcgttaatc gccgcataat atcttttctt aacaaaaact tttgtatttt acctgaggca
     7501 gtgcgcggta gtttttcgat taccacgata tgttcaggat atttatattt tgcgacccgt
     7561 ttacggctaa aaaaagccac cacctcttcc agcgataatg aatgatgcgg cgctttcagc
     7621 acgacataag cgcatgatcg ttcacctaaa cgttcatcgg gcattgcaac cacacaggca
     7681 tcgtgaattt taggatgctg caataaaata tcttccactt cacggctgct aatattttcg
     7741 ccgccgcgga caataatatc ttttttgcgt ccggtaattt ttatatagcc agcctcatcc
     7801 atacggcaga gatcgccgct gtaataccag ccttcttcat ccagcgcacg ggcggttaat
     7861 tcaggttcat caaaatatcc cataaacaca ttggggccac gcgaggcttc ttcaccttcg
     7921 caacctggcg gtaaggtctt gcgtgcgtca tcgaccactt taatctctac acctgcggca
     7981 gcgtaaccat cggtgtgcat aaagcgcgac aaaggatcat cgagattcac caccgcatgc
     8041 ggcgaacttt ctgtggaacc ataaacactt aataatttaa tgccgagctg ctggcattca
     8101 cgcgccactt ttttggggat tgtggttccg ccgcaaagaa agaaacgcag cgctgacagg
     8161 tccgcgggtt gtttctctag tacattcaaa agatcataga caaacggcgt tgcgcctagc
     8221 atacaggtgc aacgctgctg ctcaagcagc gcgagacacg catcaggagt gaaaatatct
     8281 aacaacacgc tgcgagcgcc aattaagaat ggtgccgtta cgccatgcag aaagcccgtt
     8341 gcgtggccga gtggcgcagg catcataaag acatcctgcc aggtcagatt cagccgcgcg
     8401 caataagccc gctcactggc gagaatattg ttatgcgtta gcatcacgcc ctttggcaga
     8461 ccctcggttc cggaggtaaa cagcaccgca gctaattcat cgccgtgggt cgttatcgcc
     8521 gtggtcagtg gggtattgtc ggcgataatc tgacttaatg agagggaaga ggtggcggga
     8581 gccagtttgt ccacgccgac aatttgttgt agttgtggaa gctgattttg cagcggcagg
     8641 attaaatcta ccgggcgcgt ttgtttaaac aacgtcggtg caaagaacat ttttgcctga
     8701 cacttattga gcacccacac cagttctgct tcccgccagg aaggcaacag cggcacggaa
     8761 accgcaccga ttttcaggca ggcaagatag ataacggtaa attcacacca gccaggcagt
     8821 tgaaatgcga tgcgatcgcc tgattcaata cccttcgcta acatccagtt tgccagacag
     8881 ctcgcggcgt gatcgagcgc gctataggtg tacgatgcac catgattatc gaccacggca
     8941 attttgtctg gcatcgcacg agcggtctgc tgccagtaat cggccagcga agcatcgccc
     9001 catagccctt gctgacgata cgccgcacga cgttgttcgt tgaacgttaa tgtcactttc
     9061 atgttggact ctcgaaacag aacatcaggc ccgagatgcg ggcctgtggg atgcatgatt
     9121 agccgtagcg aaaatcaata ccgaaggtgc ctgcgggata ttgccactgt tcgagaatag
     9181 tgttaccgca cagcacccga caggtgccgc actccagaca accggcagaa tcaaaatgaa
     9241 tgttgccggc gtcatcctgc ttataaagtc cggcagggca ggctttcatt aatttacgga
     9301 attcattgat atcgggattt gccgccagaa tgatatgcgg gtggccctca tcaacatgga
     9361 atttattgac gcctaatttg atgtcgacgt taaccgtagc gttctggctc atagcgcggt
     9421 tgctccctta atgccatctt tcagcaagtt gatcagccca attttcttcg catgtcccat
     9481 gatcattttg cgtaccggct ggtttggttt gccgtcaatg gtgaacatct cgttcatgat
     9541 gtcggcgacc attcgtgggt attggctaaa caggcgcggg ttttccatca gcgccgggat
     9601 cttgcggaaa tgctgcatat cgcgcatgat gcagctttgt tccagctcac gtttgtattg
     9661 cgccagactg ctggcggaga aatcctcgcg ttctttggcg gcgatcaccg ttgtggcggc
     9721 agcctgagcc gatgcaatgg ctaaatccat gccacgaact gtaaaaccca aattcaggca
     9781 gaagcctgcg gcgtcaccaa cgatcatcac gccatcgtta accatctgcg gcaccattgc
     9841 cagaccgcct tctggcacca tatgcgcgga atattcaagc agtttgccgc cgctaattag
     9901 tgggcgaatg gcggggtgtt gtttaaaatc ttccagcatt tgcggcacgc ttttttgcgc
     9961 atgggcgata tcacccaatc cacaaaccag ccccaaggat atggaatcct tgttggtata
    10021 gagaaatccc ccgcccatca ggccgtcaga aggggaaccg gcaaacagcc aggcggcacc
    10081 ttcctcgccc gtaatattaa agcgatcgtt gatctgttct ggtgtgaggc caataacctc
    10141 tttaacacca acagcgtaat gatgcggatc ggaagcggga accattccca gcgagcggcc
    10201 aagcatcgag ttaacgccat cagccagaat caccacattc gcttcgagaa tatcatcccc
    10261 agcctgcacg ccagtgacct tgtttccttc acgaaccagc gcatcgacgc gaacgcctgg
    10321 gataaactgc gcgcccgcct gctcggcttg ttccatcaac cacgggtcca gacgattacg
    10381 caatacggta taagatgcgt gttgcggaac atctggttgc tcgcggtgaa aatcgagggt
    10441 aacggcgctt tcttcggtta agaaggagat tttctcgcgt gtgaccttgc gttctaccgg
    10501 cgctgatact gcaaagcctg gaatgattgc ttcaagtgtg tgggcataaa gacgcccgcc
    10561 ggtcatgttt ttacatccgg cactgtcgcc gcgttctatc accaggacat ccagcccggc
    10621 tcgcgccatg accagtgcgg caacgctacc agcaacgccc gcaccgacca caatggcatc
    10681 aaatttgtct tccgacatgc gttatgctcc cttcccgctg cgtttacagc gggagatgtg
    10741 gttgaaaggg gaattagcgg ctcaactggc taatcagggc agggacgact ttgtagatat
    10801 cgcccaccag accgtagtcg gcataattga agattggcgc atttttatct ttattgatgg
    10861 cgacaatcac ttttgcgccg ttgccgccaa ccatatgctg gatctgcccg gagatcccca
    10921 gcgtcaggta gagatcggat ttcagcaaca cgccggagac accgatataa cgttcacgct
    10981 ccatccagtt ctcgccttcg gcaattggac gtgaacagcc gacttcagca ttcagcaccg
    11041 ccgccagttc gtggaccatt ttcaggtcat cctgcgccgc cagaccacga ccgacgccaa
    11101 ccacacgttt tgctttgctc aggtccacgc tgcttgcggc tttcgcacgg cgttcgcgac
    11161 agagaatttc atgacgcgga gcaacataag gtaccgtttc tgtcgggcac tgatgagagg
    11221 tatcactggt gcacggttcc tgaacaccgg gtgcaagggt aatgatcgcc agcgggctgt
    11281 tgatcttttc ctgagcgaac gctaacccgc catacatccg gtgttcggcg caaatgtgac
    11341 catcgacaat atccaccgcc gtggcatcgt tcaccagcgc cgcattcagt tgcacactta
    11401 accgtgctgc cagagcttta ccacgtttcg tcgcggccaa cagcaacata gcggggtgtt
    11461 tatctttcag cagggcagca atgctttcgg cgtaattttc agtgcgttgc agcgcgtcgt
    11521 tttgctcaag aacataaata cattttggac cataaggcat aactgcctgc gcctggtcgg
    11581 tattttgtac aatggcatac acctgttggc cccattgctg agcgccgcca aacagttcag
    11641 cataacgttc aggattatcg ctaaagaccc agacgctgtt taattgactc atcgtttctt
    11701 ctccgtaggc ttcagttcag ggctttcttc agatgttccg ccagctcggc aatggcctcc
    11761 ggcgaatcgt tatcgataat gatgtgctta cgttctgttt gcggcggtac gcgaatgccg
    11821 accagttcag caagtggcgc gctctggctc cagtcaatat cacttgcctg ccactgattt
    11881 accggttttt tacccgcgcc gagaatggct ttcatcgaag gaatgcgtgg cacgttaata
    11941 tcggaggtga cgcagagcac ggctggaaca gagagttcaa taacttcaac atcatcttca
    12001 agcgtgcgtt caatcaccag tgtattgccc tgacgctgaa tagcactcac tgcattaatc
    12061 accggaagtt gcagaatttc tccgaccagc aagccaacct gctgggcata aaggtcgccg
    12121 gaaccttcac caaagatcag taaatcgaag ccgatctttt caattgctgc cgccagcgct
    12181 tttgcggtat cgagaggcag tgcatgttca agttgcgcat cctgcaccaa atacaggctg
    12241 tgcggcccgc gggatagcac gtctttgcgc actttcgagt tctgcaacaa tgagccgcca
    12301 acggtcagcg cggctatctc atcgtcatct gttgcgagct ggcttgcagc ttcaatggca
    12361 ttgagatcga actggctgat tttggcgtcg gcattgtcga aattcagggt gtattctgga
    12421 gtgacaacaa tgtcctgttc ttcaggcacc agcttaaagc aggttattat tttcatgatt
    12481 tctcctgtga gtcgcatacg gaaccatctc cgatggaagg taaaaatggc ctgaaaatag
    12541 tgtgcgatag cggtgcgggg ttttcgacgg agcaagctgt tttccttgcc aacaatcttg
    12601 caaaaaaaat gcgatcgcta aaacaaaagt gcaatttgcg gcaatatagt tattccatga
    12661 ccgtacaatc ttgcattcag tgtaaataat cttgtttagc tctcgctaaa cgcggttgta
    12721 taaccgcaat acacgccgta ttttatccgg catatattgt ttttgcttgc tcatggaata
    12781 gggaggttgt catgtatcaa cgctgttttg ataacgccag tgaaacgctg tttgtcgccg
    12841 gtaaaacgcc acggctttca cgttttgcat ttagcgatga tccaaaatgg gagtccggac
    12901 atcacgttca tgacaatgaa accgagttga tttacgtcaa gaaaggggtt gcaaggttta
    12961 ccatcgattc ttcgttatat gtcgcacatg cggatgacat tgtggtgata gaacgcggca
    13021 ggctacatgc ggtggcctct gacgttaacg atccggcaac gacgtgtacc tgtgcgctgt
    13081 acggctttca gtttcagggg gctgaggaaa atcagctact gcaaccgcat tcttgtccgg
    13141 taattgccgc ggggcaggga aaagaagtca taaaaacctt atttaatgag ctaagtgtga
    13201 ttttgccgca aagtaaaaat agccaaacat cttcgttatg ggatgcattt gcctatacgt
    13261 tagccattct ttactacgaa aatttta
//
LOCUS       DAZGJW010000127        13002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZGJW010000127 DAZGJW010000000
VERSION     DAZGJW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.127"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(640..1731)
                     /gene="ychF"
                     /locus_tag="ACHKIK_003767"
     CDS             complement(640..1731)
                     /gene="ychF"
                     /locus_tag="ACHKIK_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO9084855.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1848..2432)
                     /gene="pth"
                     /locus_tag="ACHKIK_003768"
     CDS             complement(1848..2432)
                     /gene="pth"
                     /locus_tag="ACHKIK_003768"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO9084856.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2710..2988
                     /gene="ychH"
                     /locus_tag="ACHKIK_003769"
     CDS             2710..2988
                     /gene="ychH"
                     /locus_tag="ACHKIK_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO9084857.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3043..4722)
                     /gene="dauA"
                     /locus_tag="ACHKIK_003770"
     CDS             complement(3043..4722)
                     /gene="dauA"
                     /locus_tag="ACHKIK_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO9084858.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4847..5794)
                     /gene="prs"
                     /locus_tag="ACHKIK_003771"
     CDS             complement(4847..5794)
                     /gene="prs"
                     /locus_tag="ACHKIK_003771"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO9084859.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5945..6796)
                     /gene="ispE"
                     /locus_tag="ACHKIK_003772"
     CDS             complement(5945..6796)
                     /gene="ispE"
                     /locus_tag="ACHKIK_003772"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO9084860.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHASFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6796..7419)
                     /gene="lolB"
                     /locus_tag="ACHKIK_003773"
     CDS             complement(6796..7419)
                     /gene="lolB"
                     /locus_tag="ACHKIK_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO9084861.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7633..8889
                     /gene="hemA"
                     /locus_tag="ACHKIK_003774"
     CDS             7633..8889
                     /gene="hemA"
                     /locus_tag="ACHKIK_003774"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO9084862.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8931..10013
                     /gene="prfA"
                     /locus_tag="ACHKIK_003775"
     CDS             8931..10013
                     /gene="prfA"
                     /locus_tag="ACHKIK_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO9084863.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            10013..10846
                     /gene="prmC"
                     /locus_tag="ACHKIK_003776"
     CDS             10013..10846
                     /gene="prmC"
                     /locus_tag="ACHKIK_003776"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO9084864.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10843..11235
                     /gene="sirB2"
                     /locus_tag="ACHKIK_003777"
     CDS             10843..11235
                     /gene="sirB2"
                     /locus_tag="ACHKIK_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO9084865.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11239..12048
                     /gene="sirB1"
                     /locus_tag="ACHKIK_003778"
     CDS             11239..12048
                     /gene="sirB1"
                     /locus_tag="ACHKIK_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO9084866.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYVQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            12084..12938
                     /gene="kdsA"
                     /locus_tag="ACHKIK_003779"
     CDS             12084..12938
                     /gene="kdsA"
                     /locus_tag="ACHKIK_003779"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO9084867.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3263 a         3252 c         3354 g         3133 t
ORIGIN      
        1 tatatcgagt tattccctaa gttgtcgttg cttattccaa ttcgttacta taaatatggc
       61 aaaaatatta caacaaacag cggttaaaaa atttggtgaa gattttttgt aataatccta
      121 attagttatg ggaatttcgc ttgatgtatc tgcataacaa aaacaatatg cgccattttt
      181 gcattactaa taagaaaaag caaaaaacat cgatattcat ttttggtcaa tgggttttct
      241 ggcctatcgt tgttattgtg ctactggtta tcattttcat cttgccagga tcgcacgtaa
      301 tcacgttatt cggggacttt atctgcggga tattaccctt caggtaagtc cttttctcgc
      361 cagcatattg gagcgtgatc aatattgatc agctgtgaag ggggtgatgt gtagtggacc
      421 aaaacttacg ttatatcact actaatgaga tatccgtcat ctgacactga tgtctttaat
      481 tattctcgtg gttcactgta ggcaataagc acaaaagtgt aggatgttac aagaatgatt
      541 aggactcggt gaaatgaaaa atccacgcaa ttgcgtggat tatatatact tttgcgctct
      601 tcatgagatt tagcgaaacc tcatgagaca acaaattaat tagacgttga aaaggaagtt
      661 catcacatcg ccatctttaa cgatgtaatc tttaccttct gcacgcattt tgcctgcttc
      721 tttcgcgcct tgttcacctt tgtaagtgat gaaatcttca aacgagatgg tttgtgcacg
      781 gataaagcct ttttcaaaat cagtatggat tttgcccgct gcctgcggcg cggttgctcc
      841 aaccggaatg gtccatgcac gcacttcttt caccccagcg gtgaagtaag tttgcaggtt
      901 cagcagctta taaccggcac ggatcacacg gttcaggccc ggctcttcca gcccaagctc
      961 ctgcataaac tcgtcacgtt cttcgtcgtc cagttcggca atgtctgctt caacagcagc
     1021 acaaaccgga accacaacag aaccttcttt cgccgcgatt tcacgcacct ggtcaagata
     1081 tgggttgttt tcaaaaccgt cttcgttgac gttggcgatg tacattgttg gtttcagcgt
     1141 caggaagctc aggtaacgaa tagccgcttt ctcttcagcg cttaaatcca gcgcgcgcag
     1201 catacctgca ttttccaact ggggcaggca tttttccagg accgccagct cagctttcgc
     1261 gtctttatcg ccacctttgg ctttcttctg tacgcgatga atcgcacgtt cgcaggtgtc
     1321 gaggtctgcc agcgccagtt cggtgttgat aacttcaata tcgtcagccg ggttaacttt
     1381 gccggaaacg tgaatgatgt tgtcattttc aaagcagcga acaacgtgac cgatcgcttc
     1441 ggtttcacgg atgttggtca ggaactggtt acccagacct tcgcctttcg atgcgccttt
     1501 taccagaccg gcgatatcga caaattccat ggtcgtggga agcgtacgtt gcggttttac
     1561 gatttcagcc agttgatcta ggcgagggtc aggcattggt acgacgcctg tgttcggctc
     1621 aatggtgcag aatggaaagt tggccgcttc aataccggct ttggtcagcg cgttgaacag
     1681 ggtagatttc ccgacgttgg gcaaaccgac gataccgcat ttgaatccca tgatttaaat
     1741 caccttaata tcttaataat caacctgtta ttgctaacag attgcagaaa tggaaataac
     1801 tttgcctatt atacacggca ctcggcaaaa atgccgcaga caacgactta ttgcgcttta
     1861 aaggcgtgca atcggttcgt tgctttggtc aagccatctg taaaccacat ttcagtacaa
     1921 cgcgccgctt cgtcgatggc ttcatcaatt aacttctgtt cactaacagg cggtttgcct
     1981 aacacaaaac cgacaacttt atttttatcg cccggatgac cgattccgat gcgtaaacgg
     2041 tgaaagttag ggttattacc caatttactg atgatgtctt tcagtccatt gtgaccacca
     2101 tggccaccgc ccaatttaaa tttggcgacg ccaggaggca gatccagttc gtcgtgggcc
     2161 accagaattt cgtccggatt aatgcggaaa aaactggcca tcgccgcaac ggctttgccg
     2221 ctgagattca taaatgtagt cgggactaac aggcggacat cttcgcctcc aagagtgact
     2281 cgcgaagtat aaccaaagaa tttagcctct tcgcgcagcg gagcgcgcaa acgctctgcc
     2341 agtaagtcaa cgaaccaggc accagcatta tgtcgcgttg cggcgtattc agcaccgggg
     2401 ttcgccaggc cgacaatcaa tttaatcgtc acgttttttt gtcctgagtg tgtacataac
     2461 tggcgcgtag tttactggtt gcggccccgc ttgacaaaaa actgcgtatc aaatgcagat
     2521 aacgtaataa ttgcatgagt ggactattag aaagtcaagg tgttcaggcg tttatttgta
     2581 aagttttgtt gaaataaggg ttgtaattgt gatcacgccc gcacataacc cacagggtgt
     2641 tgtctatact ttacacataa ggaagagggg tattccctgt tacaacccag aaagttccgg
     2701 aggtgacata tgaaacgcaa aaacgcttcg ttactcggta acgtgctcat ggggttgggt
     2761 ctggtggtaa tggtggtcgg cgtggggtat tcaatcctca accagttacc acagtttaat
     2821 atgccccagt atttcgcaca tggtgcagtg ctaagtattt tcgtcggtgc cattctctgg
     2881 ctggcgggtg cccgtgttgg cgggcatgaa caggtgtgcg accgttactg gtgggttcgc
     2941 cactatgaca aacgttgccg ccgtagcgat aatcgccgtc atagctaaca aataatgcag
     3001 tttgctgacc agtcagtttg cgctgactgg tcaatctcgt acttataaat ccgccatcgc
     3061 cgcgcgacga ttcgggaaga acgccaggcg tcccgggatc ggttgaatgc cagcgcgagc
     3121 catagtgcgc agtggctgga attccacgtt gcacacgcgc agttcacatc cctcgggcag
     3181 acgcttcaca aaacgctgga acgcatcaag accaccagca tcaagtaccg gaaccgcatc
     3241 ccacttcaga atcacaatcc gtttgccttc aagacgtgac tccaggtccg tgaacaagcc
     3301 ttcagcagca gcaaaaaaca gcgggccaat aacgcgcaga accaggacat cgtctggaac
     3361 atctacgact accggtgcca ggcgagtcat acgtgcgata cgacgcataa acagcagcga
     3421 tgccagcacg atccccacgc tgatggcaat aaccatatca aacagcacgg tcagcgacat
     3481 gcacagcagc atgacaatga tgtcatcttt cggcgcatga cgcagcaagt cgaccacttt
     3541 gtgcgcttca ctcatgttcc acgccaccat caacagcagg gcagccatgg cggaaagcgg
     3601 cagccaggag agcagcggtg ccagtaccag cagggcaaga ataaccagaa tagagtggat
     3661 caccgccgag ataggggaag ttgccccggc acggacgtta gcggcagaac gcgcgatggc
     3721 agctgtagcg gtaatgccac caaagaacgg agcgatgata ttccccagtc cctgtccaac
     3781 cagttcgcta ttcgccttgt gtttcgtccc ggtcatacca tccagcacca cggcgcagag
     3841 cagagattcg attgcgccga gcattgccat tgagaatgcc gcaggcagca gtgtgcgaat
     3901 agaatcccag gttagcgtga attctgaatt aggcagatcc cacggcagca ccagttgcgg
     3961 cagcagttgc ggaataccgt taccctgaga accatcggcc aggacgtagt ggaattgcga
     4021 accgatggta gcaacatgtc cgccgagcag gttaacaatc cccatcaccg cgcaaccagc
     4081 cagcaaggcc ggaaggtgac cgggtaaacg aatgcccaga cgcggccaga aaacaagaat
     4141 acctagcgtc acaatgccaa tggcagcatc acccacatta atggtcggca gcgccataaa
     4201 taatgcgccg actttttgta gataatgttc cgggacatgg gccatttgca gaccgagaaa
     4261 atctttaatc tgcatggtac cgatggtgat cccgataccc gaggtgaaac ctaaggtgac
     4321 ggaaaccgga atatactcaa tcaggcgacc aaagcgtgcc agacccatca gaatcaaaaa
     4381 aatccccgac agcaaggtcg caaccagcag tcctgccagt ccaaactgtt gcgacacggg
     4441 atagagaatt accacaaatg ccgcagtcgg accggaaacg ctaaagcgtg acccacccgt
     4501 cagagcaatg acaatccccg caacagctgc ggtatataaa ccgtactggg gtgccacacc
     4561 actaccaata gccaacgcca tcgccagcgg gatagcaata atcccgacgg ttatcccggc
     4621 aatcaggtca cgggtaaacc gtgcggcagt atatttttct ttccagcaag cgtcgatcag
     4681 agcgcggaaa ggcatcacat gtgaggaaaa tattttgttc acaataatgt ttcatccgtg
     4741 agcgcatcat ctgtcaacta aatggcaggt gaaggaggca taggtcatac aaatggatat
     4801 tacagacaaa aaaacccgcc gcagcgggtc tttgagccgg gttcgattag tgttcgaaca
     4861 tggcagagat cgattcttcg ttgctgatac gacgaatcgc ttcggccagc atacctgaca
     4921 gggtcagagt acgcacgttc ggcagtgatt tgatttcatc gctcagcgga atggtatcgc
     4981 agacaacgac ttcatcaatt acagagttac gcaggttatt cgccgcgttg ccagagaaga
     5041 tcgggtgagt cgcgtacgca aatacacgtt tagcaccacg ttctttcaga gcttcagcag
     5101 ctttacacag cgtaccgcca gtgtcgatca tatcatcgac cagtacgcag tcacgacctg
     5161 caacgtcacc gatgatatgc atcacctggg aaacgttcgc acgcggacga cgtttgtcga
     5221 tgattgccat atcggtatcg ttcagcagct tagcgatagc gcgggcacgc acaacgccgc
     5281 cgatgtccgg agaaaccaca attgggttat ccagattcag ctgcagcatg tcttccagca
     5341 ggatcgggct accaaatacg ttatcaaccg gaacgtcgaa gaaaccctga atctgttcag
     5401 cgtgcagatc cactgtcagc acacggtcaa caccgacgct ggagaggaag tctgcaacca
     5461 ctttcgcagt gattggtaca cgagcggaac ggacgcgacg gtcctggcgc gcatagccaa
     5521 agtaggggat aacagcggtg atacgacctg cggaagcacg acgcagggca tcaaccataa
     5581 cgactaattc catcaggttg tcgttagtag gggcacaagt ggactggatg atgaaaatat
     5641 caccaccgcg tacattttcg ttgatttgta cactgacttc gccgtcgcta aagcgaccta
     5701 cagcggcgtc gccgagtgaa gtgtacaggc ggttggcaat acgttgtgct agttccgggg
     5761 tggcgttacc agcaaaaagc ttcatatcag gcacgagaag aacctcaggc atgcgtccat
     5821 tggtggaaag aatctgccga aaactgtgcg ggccaggcat gatcctttcc aggcggtgta
     5881 ttaaagagcg cgatgcaacg tctggaacaa ggtgacgttg tcaccgaaac tcagcttgcc
     5941 cggcttaaag catggctctg tgcaatgggg aaagattaac gcctttcgcc acaaagccat
     6001 tgagccattc cggggcttgc tctagcacct ggcgggcttc agactctgta tcaaattcag
     6061 caaagacaca ggcccctgtc ccagtcaggc gcgacggggc gtattctaac agccaggaaa
     6121 gcaccgcatc aacctcgcga aaacgttttc ttgcgataac ctcgcaatca ttgctgaatt
     6181 cacattttag caacgtttct attgaccttt ttggcgtatt gcgcgggagt tcaggatctt
     6241 taaaaatcac cggagtcgga atacttacac cagggtgcgc caccagatac cacttctctg
     6301 gcggatccac cggcgttagt atttcaccaa cgccttcggc aaacgaggca tgcccccgaa
     6361 caaagacagg aacatctgcg cccagcgtca gccccatttc cgccagctca tccatgctta
     6421 gcccgcattg ccagagatga tttaatgcca ccaggaccgt cgcggcattg gatgaaccac
     6481 cgccgagacc gccgcccatc ggcaaacgct tgtcaatgct gatattcgca ccgcttcccg
     6541 tcggaagacg cccgctgtct gccgcagttt tcatcaacaa tcgcgctgcg cgaacgatca
     6601 ggttatcttc atgttccacg ccttcaacgg gcgttaacag acgaatatcc ccatcgtcac
     6661 gaagctcaat gctgatggtg tcgccgtaat caagaaactg aaacagcgtt tgcagcgtgt
     6721 ggtaaccatc cgcacgctga ccggtaatgt ataaaaacag attaagtttt gccggagagg
     6781 gccactgtgt ccgcattatt tcactatcca gttatccatt tttaacttga tgcgttgacc
     6841 accgtcggtg agttccatat tggctggcat cgcaggttgc gttttggtgt cataaccacc
     6901 ataaacaacc ttccagtttt tgccattctg gctgtaggta atttcgctca ggcggtactg
     6961 gtcgtccagt ttgtagtcgg ttgcatcacc cggtaaacct aaaatccact ggcgcaagct
     7021 gttgagcgga attggcattc cggtcaattt gccaatcatc tcttcggcgt catcggcggt
     7081 ataacgctga cctttattgt cgactaactg cacgttaccc ggttgagcat tcagctccag
     7141 ttccgtgctg cccaatgggt tagtgagcag cagacggtag cgatcctggc cggtttgctg
     7201 ccagaaaaag cgggcgtaca ctttttgttg gtcagaaata taagcgaacg cgccgcgagt
     7261 ctgatactga ttaagattgc gcacgtcttg ctgatgctga cgccattgtg gcgaatccgg
     7321 gcttttgcca ggacctttgg gcgtggtaac ggaacaggca gtgagcacaa gagcagccag
     7381 cggtagcagg cggataagac gaaaatcggg caggggcata gtgatgacaa gtccttgaga
     7441 tacgttgcag ttataaccct taatgctagc gttaccgtcc gctatcgtct atgttcaagt
     7501 tgtcttaatt gccagaatct aacggctttc ggcaattact ccaaaagggg gcgctctctt
     7561 ttattgatct tacgcatcct gtatgatgca agcagactaa ccctatcaac gttggtatta
     7621 tttcccgcag acatgaccct tttagcactc ggtatcaacc ataaaacggc acctgtatcg
     7681 ctgcgagaac gtgtatcgtt ttcgccggat aagctcgatc aggcgcttga cagcctgctt
     7741 gcgcagccga tggtgcaggg cggcgtggtg ctgtcgacgt gcaaccgcac ggaactttat
     7801 cttagcgttg aagagcagga taacctgcaa gaggcgttaa tccgctggct ttgcgattat
     7861 cacaatctta atgaagaaga tctgcgtaaa agcctctact ggcatcagga taacgacgcg
     7921 gttagccatt taatgcgtgt tgccagcggc ctagattcac tggttcttgg ggagccgcag
     7981 atcctcggtc aggttaaaaa agcgtttgcc gattcgcaaa aaggtcatat gaaggccagc
     8041 gaactggaac gcatgttcca gaaatctttc tctgtagcga aacgcgttcg cactgaaaca
     8101 gatatcggtg ccagtgctgt gtctgtcgct tttgcggctt gtacgctggc gcggcagatc
     8161 tttgaatcgc tctctacggt cacagtgttg ctggtaggcg cgggcgaaac catcgagctg
     8221 gtagcgcgtc atctgcgcga acataaagta cagaagatga ttatcgccaa ccgcactcgc
     8281 gaacgtgccc aaattctggc agatgaagtt ggcgcggaag tgattgccct gagtgagatc
     8341 gacgaacgtc tgcgcgaagc cgatatcatc atcagttcca ccgccagccc gttaccgatt
     8401 atcgggaaag gcatggtgga gcgcgcatta aaaagccgtc gcaaccaacc aatgctgttg
     8461 gtggatattg ccgttccgcg cgatgttgag ccggaagttg gcaaactggc gaatgcttat
     8521 ctttatagtg tggacgatct gcaaagcatc atttcgcaca acctggcgca gcgtaaagcc
     8581 gcagcggttg aggcggaaac tattgtcgct caggaaacca gcgaatttat ggcgtggctg
     8641 cgagcacaaa gcgccagcga aaccattcgc gagtatcgca gccaggcaga gcacgttcgc
     8701 gatgagttaa ccgccaaagc gttagcggcc cttgagcagg gcggcgacgc gcaagccatt
     8761 atgcaggatc tggcatggaa actgactaac cgcttgatcc atgcgccaac gaaatcactt
     8821 caacaggccg cccgtgacgg ggataacgaa cgcctgaata ttctgcgcga cagcctcggg
     8881 ctggagtagc agtacatcat tttctttttt tacagggtgc atttacgcct atgaagcctt
     8941 ctatcgttgc caaactggaa gccctgcatg aacgccatga agaagttcag gcgttgctgg
     9001 gtgacgcgca aactatcgcc gaccaggaac gttttcgcgc attatcacgc gaatatgcgc
     9061 agttaagtga tgtttcgcgc tgttttaccg actggcaaca ggttcaggaa gatatcgaaa
     9121 ccgcacagat gatgctcgat gatcctgaaa tgcgtgagat ggcgcaggat gaactgcgcg
     9181 aagctaaaga aaaaagcgag caactggaac agcaattaca ggttctgtta ctgccaaaag
     9241 atcctgatga cgaacgtaac gccttcctcg aagtccgagc cggaaccggc ggcgacgaag
     9301 cggcgctgtt cgcaggcgat ctgttccgta tgtacagccg ttacgccgaa gcccgccgct
     9361 ggcgggtaga aatcatgagc gccagcgagg gtgaacatgg tggttataaa gagatcatcg
     9421 ccaaaattag cggtgatggt gtgtatggtc gtctgaaatt tgaatctggc ggtcatcgcg
     9481 tgcagcgtgt tcctgctacg gaatcgcagg gtcgtattca tacttctgct tgtaccgttg
     9541 cggtaatgcc agaactgcct gacgcagaac tgccggacat caacccagca gatttacgca
     9601 ttgatacttt ccgctcgtca ggggcgggtg gtcagcacgt taacaccacc gattcggcaa
     9661 ttcgtattac tcacttgccg accgggattg ttgttgaatg tcaggatgaa cgttcacaac
     9721 ataaaaacaa agcgaaggcg ctttcagtgc tcggtgctcg catccacgct gctgaaatgg
     9781 caaaacgcca acaggccgaa gcgtctaccc gtcgtaacct gctggggagt ggcgatcgca
     9841 gcgaccgtaa ccgtacttac aatttcccgc aggggcgcgt taccgatcac cgcatcaacc
     9901 tgacgctcta ccgcctggat gaagtgatgg aaggtaagct ggatatgctg attgaaccga
     9961 ttatccagga acatcaggcc gaccaactgg cggcgttgtc cgagcaggaa taatggaata
    10021 tcaacactgg ttacgtgaag caataagcca acttcaggcg agcgaaagcc cgcggcgtga
    10081 tgctgaaatc ctgctggagc atgttaccgg cagagggcgt acttttattc tcgcctttgg
    10141 tgaaacgcag ctgactgacg aacaatgtca gcaacttgat gcgctactga cacgtcgtcg
    10201 cgatggtgaa cccattgctc atttaaccgg ggtgcgagaa ttctggtcgt tgccgttatt
    10261 tgtttcgcca gcgaccttaa ttccgcgccc ggatacggag tgtctggtgg agcaggcact
    10321 ggcgcggttg cctgaacaac cttgccgtat tcttgatctc gggacgggta ccggggcgat
    10381 tgcgcttgcg ctggctagcg agcgcccgga ctgcgaaatt atcgctgtag atcgtatgcc
    10441 tgatgctgtc tccctggcac aacgtaatgc ccagcatctg gcgatcaaaa atatccacat
    10501 tctgcaaagc gactggttta gcgcgctagc cgggcagcag tttgcgatga ttgtcagcaa
    10561 tccgccgtat attgacgagc aggacccaca tcttcaacaa ggcgatgtcc gctttgagcc
    10621 gctcactgcg ctggttgcgg cagacagtgg aatggcagac atcgtgcata tcatcgaaca
    10681 gtcgcgtaac gcgctggtat ccggcggctt tctgcttctg gaacatggct ggcagcaggg
    10741 cgaagcggtg cgacaagcat ttatcctcgc ggggtatcat gacgtcgaaa cctgccgtga
    10801 ctatggtgat aacgagcgcg taacgctcgg ccgctattat caatgacaag tttttctaca
    10861 ctgcttagtg ttcatcttat tagtatcgcg ctttctgttg ggctattaac cttacgtttc
    10921 tggctacgtt atcagaagca tccacaggca tttgctcgct ggacgcgcat tgtgccgccg
    10981 gttgtcgata cggtgttatt gttaagcggc attgcgttga tggctaaagc gcacatcctg
    11041 ccattttccg ggcaggcaca gtggctgact gaaaagctgt ttggagttat catttatatc
    11101 gttttgggtt ttattgcact cgattatcgt cgtatgcaca gtcagcaggc gcgcattatt
    11161 gccttcccgc tggcgttggt ggtgctgtac atcatcatta aactcgccac cacaaaagta
    11221 ccgttactgg ggtaagtcat gagatcgtta gctgatttcg aatttaataa agcgccattg
    11281 tgcgaaggca tgatcctggc ttgcgaagca atccgccgcg attttccctc gcaagatgtt
    11341 tacgacgaac tggagcgtct cgttagtctg gcgaaggaag aaatcagcca gcttctgcct
    11401 ttagaagagc agctggaaaa actgatcgcg ctgttttacg gcgactgggg atttaaagcc
    11461 tcacgcggcg tttatcgtct ttccgatgca ttatggctgg accaggtgtt aaagaatcga
    11521 cagggcagtg cggtatcatt aggtgcggtt ttattatggg tcgcgaatcg tctcgatttg
    11581 ccgctgctgc cggtgatttt ccctacgcag ctgatattgc gcattgaatg tccggatggc
    11641 gaaatttggc tgattaatcc ttttaacggt gaatcgttaa gcgaacatat gctggacgta
    11701 tggttaaagg gaaatatcag cccgtcggcg gaactgtttt atgaagacct tgatgaagct
    11761 gataacattg aggtaatccg caaattgctg gatacactca aagcctcgtt gatggaagaa
    11821 aatcagatgg agctggcgtt acgcaccagc gaagctttat tacaattcaa ccctgaagat
    11881 ccctatgaaa ttcgcgatcg cgggttgatt tatgtgcaac tggattgcga acacgttgcg
    11941 ttgaacgatt taagttattt cgttgaacag tgtccggaag acccgatcag cgaaatgatc
    12001 cgtgcgcaaa taaataacat cgcgcataaa catattgtgc tgcattaatt aatcgacatt
    12061 ttactcaaga ttaaggcgat cctatgaaac aaaaagtggt tagcattggc gacatcaacg
    12121 tagcaaatga cctgccgttc gtactgtttg gcggtatgaa cgtgctggaa tctcgcgatc
    12181 tggcgatgcg catttgcgag cactacgtaa ctgtgaccca gaaactgggt atcccttacg
    12241 tgttcaaagc ctcttttgac aaagccaacc gctcctccat ccactcttat cgtggaccgg
    12301 gcctggaaga agggatgaaa atcttccagg agttgaagca gacttttggc gtgaaaatta
    12361 tcaccgacgt tcacgaacca agtcaggcac agcccgttgc tgatgtcgtg gatgtgattc
    12421 agttgccggc gtttcttgct cgccagactg acctggttga agccatggcg aaaaccggtg
    12481 cggtaattaa cgtcaagaaa ccacagtttg tcagcccggg acagatgggt aatatcgttg
    12541 ataaattcaa agaaggcggc aacgaaaaag tgattctttg cgatcgtggt gctaacttcg
    12601 gctatgacaa cctggttgtc gatatgctgg gcttcagcat tatgaagaaa gtgtctggta
    12661 actcgccggt gattttcgac gtgacccacg cactgcaatg ccgcgatccg tttggcgcag
    12721 cttccggtgg tcgtcgtgct caggtggctg agctggcacg agccggtatg gcggtaggtc
    12781 tggcggggct gtttattgaa gcgcatccgg atccggaaca tgcgaaatgt gatggtccat
    12841 ccgcgctgcc gctggctaaa ctggaaccgt tcctcaagca gatgaaagcg attgatgatc
    12901 tggtgaaagg tttcgaagaa ctggatacca gcaagtaatc ttttttgctt gaaaaataaa
    12961 gtcttagcgt tctgcgttaa gacttttttc atgggtgcca ga
//
LOCUS       DAZGJW010000128        12739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZGJW010000128 DAZGJW010000000
VERSION     DAZGJW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.128"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1087)
                     /gene="umuC"
                     /locus_tag="ACHKIK_003780"
     CDS             complement(<1..1087)
                     /gene="umuC"
                     /locus_tag="ACHKIK_003780"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HFO9084868.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMLLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLSLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLF"
     gene            complement(1087..1524)
                     /gene="umuD"
                     /locus_tag="ACHKIK_003781"
     CDS             complement(1087..1524)
                     /gene="umuD"
                     /locus_tag="ACHKIK_003781"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO9084869.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(1521..1769)
                     /locus_tag="ACHKIK_003782"
     CDS             complement(1521..1769)
                     /locus_tag="ACHKIK_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO9084870.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            2187..3089
                     /locus_tag="ACHKIK_003783"
     CDS             2187..3089
                     /locus_tag="ACHKIK_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO9084871.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDEVREIITVHFTAKRSDWCDIWGNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLDGVPSAYHWYTEQYGVKWPCGYEVNISSQGENFIQVDF
                     DTPWCQPESDVIAALSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVIGPAWIVDNVAHYGG"
     gene            3086..3397
                     /locus_tag="ACHKIK_003784"
     CDS             3086..3397
                     /locus_tag="ACHKIK_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084872.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRPASVTVYASPSPFSDERPSSRFRGIALPSERRRLRYSTVGLTRYRT
                     R"
     gene            3473..4156
                     /locus_tag="ACHKIK_003785"
     CDS             3473..4156
                     /locus_tag="ACHKIK_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO9084873.1"
                     /translation="MSRFILGDCVRVMATFPDNAVDFILTDPPYLVSFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFVAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPHLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCIAALQSGRRYIGIELLEQYHCAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            4157..4378
                     /locus_tag="ACHKIK_003786"
     CDS             4157..4378
                     /locus_tag="ACHKIK_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084874.1"
                     /translation="MNYAGHEKLRAEVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDACFKD"
     gene            4308..4825
                     /locus_tag="ACHKIK_003787"
                     /pseudo
     CDS             4308..4825
                     /locus_tag="ACHKIK_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            4876..5652
                     /locus_tag="ACHKIK_003788"
     CDS             4876..5652
                     /locus_tag="ACHKIK_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084875.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLAGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEEEIRAVLDAVRDAVCSVSCRVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDYPCWLDEDDENLPVVLDDILNRGARFSAVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARTEIRSMADALAKIRK"
     gene            6068..6493
                     /locus_tag="ACHKIK_003789"
     CDS             6068..6493
                     /locus_tag="ACHKIK_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9084876.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIIVTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            6540..6962
                     /locus_tag="ACHKIK_003790"
     CDS             6540..6962
                     /locus_tag="ACHKIK_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO9084877.1"
                     /translation="MYCTVKEIIREVLDTDVSDSECVFAVVLTRGDVRHIARDWSLTD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRTSRHVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            6959..7150
                     /locus_tag="ACHKIK_003791"
     CDS             6959..7150
                     /locus_tag="ACHKIK_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084878.1"
                     /translation="MNISTETREILRKYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGQYILQGSRNR"
     gene            complement(7479..7630)
                     /locus_tag="ACHKIK_003792"
                     /pseudo
     CDS             complement(7479..7630)
                     /locus_tag="ACHKIK_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8142..8372
                     /locus_tag="ACHKIK_003793"
     CDS             8142..8372
                     /locus_tag="ACHKIK_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084879.1"
                     /translation="MTVSIVSPSSAAVKPRRHPRFRREDIPAPDIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            8424..9785
                     /locus_tag="ACHKIK_003794"
     CDS             8424..9785
                     /locus_tag="ACHKIK_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO9084880.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAESRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAETEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            9832..10395
                     /locus_tag="ACHKIK_003795"
     CDS             9832..10395
                     /locus_tag="ACHKIK_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO9084881.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVM
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVESNSGLVQYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTSAPTMIIRLRA"
     gene            10395..10652
                     /locus_tag="ACHKIK_003796"
     CDS             10395..10652
                     /locus_tag="ACHKIK_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084882.1"
                     /translation="MTPGRREDKCPFARARKRPRAGGAERGRAHAPPLSLRGRSAVGF
                     CLFRPVICLLLMMLFLLLLLLLLLLLLLLLLLLLFFFFLLL"
     gene            10547..10822
                     /locus_tag="ACHKIK_003797"
     CDS             10547..10822
                     /locus_tag="ACHKIK_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084883.1"
                     /translation="MPVADDVVPATATATATATATATATATVLLFPAALILTTAVLSC
                     LPGAPSAPGCLAASGRQARLRQAAAGAPASLRRTRWRGFGLSLRSDA"
     gene            11256..11333
                     /locus_tag="ACHKIK_003798"
                     /pseudo
     CDS             11256..11333
                     /locus_tag="ACHKIK_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            11947..12306
                     /locus_tag="ACHKIK_003799"
     CDS             11947..12306
                     /locus_tag="ACHKIK_003799"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084884.1"
                     /translation="MLPDELEKRLDGIEDASKKGYPVRNLYKIACEPGLWLQAYVNIR
                     ANAGALTQGVSTDTIDGFSIERAEKLAKTLRSREYVAKPVRRVQIPKKDGKTRPLGIP
                     GGDDKLAQEVARIILER"
     gene            12358..12739
                     /locus_tag="ACHKIK_003800"
                     /pseudo
     CDS             12358..12739
                     /locus_tag="ACHKIK_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     misc_feature    12639..>12739
                     /locus_tag="ACHKIK_003800"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         2822 a         3432 c         3711 g         2774 t
ORIGIN      
        1 caaataatcc cggctgcgct atacccgatg gtgtgaaatc agccagcatg actcctgcct
       61 tcatatagcg gtacccttca cgccagacat ggtttaaggc tctgcatgcg gcggcaataa
      121 tgtcccggct gtcctgtgtg ggcaatgaaa gcttttccac agcggcattg ctgtaacagg
      181 gttcttttac tgcaaagggg gatgtccgta caaatgtcgt cacctgccgg caatactgac
      241 gctccccacg tagtttctct gcggcccgct cagcatactg aacaacagcc tggtgcatgg
      301 catctttgtc tgtgattcgt tcaccaaaac tgcgactaca gacaatctgc tgttttgccg
      361 gtggtgcttc ttccagggat atgcaggact cgccgttgag ttcgcgtacc gtacgctcaa
      421 gaatgacgct gaagtttttc cggatgaatg ccgtgttagc ctgcgccagc tgcagtgctg
      481 tgttaatacc cagcgcattc agcttttccg tcagtctgcg tcctactccc cagacctcac
      541 caactggctg cagccccagt agcttcaaga tccgattacg gttttctgcc gtcagcgcga
      601 ccacaccgga aaactgtggc cattgctttg ttgcccactg tgcactttta gccagcgttt
      661 ttgtaggcgc aatgcccacc cccatggtga gtcctgtcca gctctttact tgttccctga
      721 gctgatgacc aaaaaactcc ggagagatgc aatgatttat cccccgcaaa tcaatgaaca
      781 tttcatcaat tgagtagggc tcaactgcgg gagaaagcga ctccagaaca gccatgaccc
      841 gttggctcat gctgtggtac agcgcataat tgctggaaaa tacatgtatt ttcttctcca
      901 ggagcatctg tctcacctga aaccagggct gccccattct gatgccaagg gcttttgcct
      961 ccgggctgcg cgcgatcaca cagccatcgt tattgctgag tacgatgacc ggttcgttgc
     1021 gaaggtccgg gcggaaaact ttttcacatg aggcgtagaa actgttgata tcagccagtg
     1081 caaacatcag cgtaactccc gcgtcctgtg tatcacgtga gtgacaacac caaaaataca
     1141 gatgttttcc ggatacagtg tgcggaattc cgggctgtct gaaaccggct ccagtgccgg
     1201 gcgtgggcgc aacagcagtc gtttgacggt gaactcaccg tcgatctcag cgataacgat
     1261 gtccccgtgt tgtggttttt cggccctgtc cactaccagc agatcaccat tctgcacgcc
     1321 agcctggttc atcgattcac cgctggcgcg cagaaagaag gtggctgcag gtctgctgat
     1381 gcaatagctg ttcagatcca gttcctgctc agcataatca gtggcaggcg aaggaaaacc
     1441 ggcctggcaa cgatcggcaa acaacggtcg cacgtaactg tcatcgcctg aagggtcagc
     1501 cggacggtga taaacggtac tcatgctgca ggcagccagc tgtcgtcctg ccagatttct
     1561 tccagcagag acaggatacg ttctttatcc ttgtcgttgc gggctcctgt gacagataca
     1621 gacggggcgc tgccctggtc tatacgcagc cagatttccg ggtagtgtgg tttcagacgt
     1681 tgttcgattt cattctgtag cgcctgaagt gtgcctgatt tgaggttttt tgtgctctgg
     1741 cggtcgaaaa gaatttcaat gcggatcatg ttctcatctc ccttttctat tgctgtatgc
     1801 atatacagta ttctaataac tgaatccgca cacagtcaaa tgtcattgct ctgaccaaaa
     1861 aacgctcaat tctctgagag tgtggctgta tgcctgctgt ggagtgctgt gtccagctta
     1921 tccacagcat tttgtgcacg gttctgtgga caaaatacct ggttacccag gccgtgccgg
     1981 cacgttaacc gggctgcatc cgatgcaagt gtgtcgctgt cgccggcctc ctcacccggt
     2041 cacgtttcgt cgttcctcct ccacgcgctg cggcttcggg gccgcacctg cattcgtatg
     2101 cggtcgcccg gttacaggtg cggcacggcc tgatggaggc cgcatgtgag aggagaattc
     2161 ccatgccaaa ctggtgctcg aatcgtatgt atttttctgg tgaaccggca cagatcgctg
     2221 agattaaacg actggccagc ggtgcagtca caccgcttta tcgccgcgcc acaaatgaag
     2281 gtattcagct gtttctggcc ggaagtgccg gacttctgca gatcactgaa aatatccgct
     2341 ctgaacagtg tcccggagtg acggctgccg gacgtggtgc tgtatcgacg gaaaatatcg
     2401 cgttcacccg ctggctgaca cacctgcagg acggtgtact gctggatgag caaaactgcc
     2461 tgatgctgca tgaactctgg ctgcagagcg gtactggaca gcgtcgctgg gaaggattac
     2521 cggatgaggt cagggaaatc atcactgtgc atttcaccgc aaaaagaagt gactggtgtg
     2581 acatctgggg taacgaggat gtatcggtgt ggtggaaccg tctgtgtgac aacgtactgc
     2641 cggaaaaaac catgccgttt gacctgctga cggttctgcc gacccgcctg gatgttgaag
     2701 tgaacggctt taacggtggt gttctggacg gtgttccttc tgcatatcac tggtatacgg
     2761 aacagtatgg cgtgaagtgg ccttgtgggt atgaggtgaa tatcagcagt cagggagaaa
     2821 acttcattca ggtggatttc gacacgccgt ggtgtcagcc ggaaagcgac gttattgcag
     2881 cattaagccg ccgtttcagc tgcactctgg agcactggta tgccgaacag ggctgtgatt
     2941 tctgtggctg gcagttgtat gagcgcggag agctcgttga tgtgctgtgg ggggaactgg
     3001 aatggtcttc cccgacagat gacgatgagc tgccggaagt catcggacct gcgtggatag
     3061 tcgacaatgt ggcgcattat ggcggatgaa gtatgacgga gacgggcggg caaccgcccg
     3121 tttcttttcc gacaaaggat gtcgccgtgc tcctcttttt actgcgccgg ctgacgcggc
     3181 gcggcataaa cgctgcctgt ggtcagtgtc ccgcgtccgg cgggcacggg acgggcgctt
     3241 ttaccgctcc cggccggcgt cggtgacagt gtacgccagc ccgtcgccct tttctgatga
     3301 acgcccttca agccgctttc gcggcatagc cttgccgtca gaaagacgac ggctgcggta
     3361 ttccacggtc ggcctgaccc gttaccggac gcggtgaaca gcccacaggc agcggggaac
     3421 gggcaccaca ggggtgccct cccggtgccc tctgtaagag aaggaggctt ttatgtcccg
     3481 ttttatcctg ggtgactgcg tgcgtgttat ggccacgttt ccggacaatg ctgtcgattt
     3541 catcctcacc gacccgccat atctcgtcag tttccgtgac cgtcaggggc gtactatcgc
     3601 cggcgataaa accgatgaat ggctgcaacc ggcctgtaat gaaatgtacc gcgtgctgaa
     3661 aaaagatgcg ctgatggtca gcttctacgg ctggaaccgt gtcgatcgct ttgtggccgc
     3721 ctggaaaaat gcgggattca gcgtggttgg tcacctggta tttaccaaaa actacacatc
     3781 gaaggccgca tatgtgggct atcgccacga atgcgcctac atcctggcaa aaggtcgtcc
     3841 acatctgcca caaaacccgc tacccgacgt gctgggctgg aaatattcgg gcaaccgcca
     3901 tcacccgacg gaaaagcccg ttaccagcct gcaaccactg attgagagct tcacacaccc
     3961 gaacgcgatt gtgctggacc cgtttgcagg cagcggctca acctgcatcg ccgccctcca
     4021 gtccggacgc cggtatatcg gtatcgagct gcttgagcag tatcactgtg ccgggcagca
     4081 acgccttgcc gccgtgcagc gggccatgca gcagggggcc gcgaatgata actggtttga
     4141 accggaggcg gcgtaaatga actatgccgg acacgaaaaa cttcgcgccg aagtggcgga
     4201 ggtggctaat accatgtgtg accttcgggc caggctgaac gatatggagc atcgctgtcg
     4261 ctttgattcc gatgtgcttg ttgaacgcct gacacgccag acgctgtatc gcgccaatcg
     4321 cctgttcatg gaggcatata ccgaaattct tgaactggat gcgtgcttca aagattaagg
     4381 aggagacgaa atgtacggaa catgcgaaac gctctgccgt gagctggcag caaagtatcc
     4441 gggaaacacg ccgctgatgc tgcttatctg gtctccggaa gagattcagg ccctcgctga
     4501 cggaatggat atttccctgt ccgatcatga aatcaggact gtcctggcgc gcctggagga
     4561 catcccggaa gaccagcgga ctgaatccgg tatttcttcc gccgctgcga tggagattat
     4621 cagcaacgtg agcgaaaacc gccaggtgac cgtctctgct gaactcctgg cgtccctgat
     4681 tcagaccgct gaacaggctc tgtggaaacg tgaatgggcc gcccgtgata acggccttgc
     4741 cgtcccggaa tgtgttaccc gccgtcaggc agtgattaat caggtccgca ccctgctgaa
     4801 aaacaacaca cacgaaaaca actgatgtta tcgccgcctg ccctgcgggc ggcgatacag
     4861 ggagaacgct taatcatgaa cgaaaacagc acgctgaacg cactgatttg tcgtcacgcc
     4921 cgcaacctgc tgctggcgca gggctggccg gaagagacgg atgttgacca gcgtaacccg
     4981 aagtatccgg gctggatcag catttatgtt ctgctggatg cgccccggct ggcgacctta
     5041 cttatcaacc gtcacggcgg cgtactgccg ccgctcctgg cctctgccat tcagaaactg
     5101 gccggaaccg gagcggaact tgttctgtcc ggcagtcagt ggcagtcgct gccggtactt
     5161 ccggcagacg gaacgcaggt gtctttcccg tatgccggtg aatggctgac ggaggaagaa
     5221 atcagggcgg ttcttgatgc ggtgcgcgat gcagtatgca gtgtcagttg ccgggtggca
     5281 gaagatgcgc ggcgtatccg tgcggcgctg accaccaccg gtcagacgtt gctgacccgc
     5341 cagacgcgcc gctttcgtct ggttgtgaag gaaagcgatt acccctgctg gcttgatgaa
     5401 gatgacgaaa atctgcccgt ggtgctcgat gacatcctga accggggagc acgtttttcg
     5461 gcggtggaaa tgtacctggt cagcgattgt attgagcata tcctgtccag tgggctggcc
     5521 tgcgatgtgc tgcgtatacc cgatgaaccg ccccgccgct ggtttgaccg tggtgttctg
     5581 cgggaggtgg tccgggaagc ccggaccgaa atccgcagca tggcggatgc cctggcaaaa
     5641 atccggaaat gatgacgctg gcggggaaaa atctccgcct tttccagccg gtccccggaa
     5701 agagcacaaa ggaagtcgcc gccggctctg ctttacccgg ccatgcgggc atggccttat
     5761 ggggtttcag ttatgtggcc tcagcgtcgt gtgcgggctg tgccgtgcct ccatcgtagc
     5821 cgggcttctg cgggcgggtt gcaaggtaag gtgaaccgct gcggtcaccc ggtccctcct
     5881 tcccgtctgc cgtgattttc cggtcgttca cccgctcaga tttcgcggtc tcaccctcca
     5941 actcccgcca gccgtgtgcg tcaggctgcg gcttcccttg cgcccttgca accctgcagg
     6001 cccggcaaag atggaggcac ggcaccgccc ggtgtcgcgg aatatgtgaa caacggagga
     6061 aaacatcatg caatatgcga aacctgtcac tctgaatgtt gaagagtgcg accgtctctc
     6121 ctttctgcct tacctgtttg gcctggattt tctgtatgcc gaggcatctg tgtacgcgct
     6181 ggcgaaaaaa atgatgccgg aatatgaagg cggattctgg cacttcatcc gcctgccgga
     6241 cggtggcggt tacatgatgc cggatggcga ccgttttcat ctggtcaacg gtgaaaactg
     6301 gtttgaccgt acggtcagcg ccgatgccgc aggcatcatt gtgacctccc ttgtgattaa
     6361 ccgccagttg tggctgtacc acgacagcgg ggatgcaggg ctgactcacc tgtacaggat
     6421 gcgcgatgcg cagttgtgga gccacatcga atttcaccct gaatgcaacg cgatttacgc
     6481 ggcgctggac tgattaacgg acggggcggc aatgccgccc ctgtaaagga gacgagaaca
     6541 tgtactgtac tgttaaagaa attatccgcg aagtgctgga tacagacgtg tcggacagtg
     6601 aatgcgtttt tgccgtggtg ctgacccgtg gggatgtgcg tcacatagcc cgggactgga
     6661 gtctgacaga cgatgagctg gaaaccgtca tgcagcggct ggacgatgcc tttgagcatg
     6721 gtgcggatgt cagcgttgtt cacgacgttg ttcgtgaact gatggaagaa aagcgcacca
     6781 gccgtcatgt gacagtcccg gcggtgatgc tggaaaaagt gatggcgctg gcaggcagtg
     6841 aaatgaagcg cctgtatgcc gtcgggagtg agaacggggg cgacggtgac gcattcgtca
     6901 gggaggaacg cgaagcaatg gacgttgtgt tacaggcgct ggacggggag acgatgtcat
     6961 gaatatcagc acggaaaccc gcgaaattct gcgtaaatac aaagccgtga ttaatgcgcg
     7021 gcgtcgtgag atggggcaga aaccgctcac caccgcacag attgttgatg aaatctgcga
     7081 ttttgtggcg aatcagcagg ctgttttcct cggcggtcag tacatccttc agggcagcag
     7141 aaacaggtga tacaggggca ggcggtggag gccgcctgtc agcccgcgcg aaaacccctg
     7201 gcaggctgac gcctgccgga agggtaatgt gtctggctca ggagtgtttc tttctgatgt
     7261 tatgactctg ttcctgatgt tgttgttgtt cttttatttg ctgctctgat tatgacttct
     7321 ggtgtgttgt tctgtaagcc ggccgcccct tcggctcccg gctgcctggc ggcatccggt
     7381 cgtcaggccc gactccgaca ggcagctgct ggcgcacccg ccagtctccg ccgaacccgc
     7441 tggcgcggtt tcgggctgtc gctacggtcc gatgcgtgaa aacccgggct tgcagcgacc
     7501 gttccggtcg cgccgcctct gtcgcgccgt ggcctgcgct cctggcatcc cggcaaaagg
     7561 ccgccgggat gtaaggggca acgtcgtttc gcgcacgcgc tctccgtcgt tgcggcgggt
     7621 ccctgctcat tgcctgccgg gtgccacaca cccgtttgag tccctgtcgc cgggggctga
     7681 tacagccaca acctgccgca ctgactgtcc agggtaaatg aacagcccac aaaatttttt
     7741 acccaaaacc cggtaaaaaa ttttgcgttc tgcccaggac aggtgcgtca ggccgtggca
     7801 gtgatgcccc ttgcggcgaa ggttctcaaa cgggtgaagt tcataccaca gggaatacag
     7861 cagcaggttc ccgcgaaacg tcaaaaccgc ggctcccgaa actcaacggg tgatgttctt
     7921 taaaaatcag gatttatttc aggctccggc aatacgcatt tatttgttct gtcagtcatc
     7981 cggctggcag gtaatgagaa cgcaggaaca gaaggaggaa aacaccggaa agcgtcagat
     8041 gcagatatga aaaaagcagg tcagggacct gcttaatcca tgccgcagag actgcacgaa
     8101 cagtttttgc ggctccagtc aacagtcata aggatgcatt tatgacagta agtattgttt
     8161 caccttcctc agcggctgta aagccccgcc gtcatccccg tttcagacgc gaagacatcc
     8221 ccgcgccgga cattgacccc gtgctgaaag ccttcgggcg acacatagcc cggagttttc
     8281 accgggggcg tggtgtacac atccccgcca tgaaaaacac ggccttcggg caggtgctcc
     8341 gcacactcga actgaaacgc gcttttaact gatgaggtga gccgccccgc gaggggcggc
     8401 aacagacgga aggagaacat tttatgacat tagcagcaat caccatgacc gccccggaag
     8461 ctgccagccc tgtgcagatg taccgcgcga cctactcacc ggatgacaac aaactgcgcc
     8521 tgtatgccga gtcacgcctc gacccggaga cgtataaaaa agtccatgat gccggtttcc
     8581 gctgggcacc aaaacaggcg ctgtttgtcg cgccagcctg gacaccgggc cgggaagacg
     8641 tgctcctctc acttgccgga gagattgagg atgaagacag cacgctcgct gaacgtcagg
     8701 aagcacgggc ggagcggttt accggataca gcggaaagcg ggccagtgaa tccgcacagg
     8761 cacttgatga agtggaaaga ctggccgcga tgatcccgcc cggtcagccc attcttgtgg
     8821 ggcatcacag cgaacgccgc gcccgtcgtg atgcgcagcg tattgaaaac ggcatgaaac
     8881 gtgccgtgat gctctttgaa cgtgcggaat actgggagga gcgggcgcgg tcggcactgc
     8941 ttcacgcgaa gtataaagaa cgcccggacg ttcgctggcg tcgtatcaaa aaaatcgaag
     9001 ctgatttgcg caaggctgaa aagaccatcg cgcagtcgca gaaatatctg acgatgtggc
     9061 gggctgaatc gctggatctg aatatggcaa aactcatcag cagtcatgac catatcagcg
     9121 cctgtttccc gctggatacg tatccgcgcc cggcagaaaa aagccagtat gaaggaagtc
     9181 gatcgttatg gtcggccctg gatgatgaca tcatcaccac ggagcaggcc cgcgaaattg
     9241 cgatccgctg tcatgaacgg cagattcagc atcagcaacg ctgggttaac cactatcaga
     9301 accgcctgat ctatgagcgt gccatgctgg acgaaagcgg cggcgtggtt acccggacac
     9361 aggattttga gccgggcgga caggttttca gccggggcga gtggctgacc atcatccgcg
     9421 tgaacaaaag caacggggcg gtgagttcag tcacaacgcc gaattacagt tttctcgggt
     9481 acagcggcac gatgaaagtg acgcccgatc gcatcacgga ctacaaagca ccatcggcag
     9541 aagaggctgc cgtcgccagt caggccgcga agcgtccgcc ggtagtcaac tatccggggg
     9601 aaggtttccg ggaaatgaca aaggcgcagt gggccgccct gccccgggac tgtaaggccg
     9661 tgcgcagtgt ggcagagaca gaagaccacg gggcataccg ctaccgccgc acaatggaca
     9721 ataatttccg cctggtgaat gtgtatatta ccgacatgaa aatcacggaa atcccacaga
     9781 aataaggtat atccccccgg gaaatccccg ggggatgacg gagataatga catgcacagt
     9841 cagttaagag aacgtatccg gctgatgcgc gcaaggctgg ataacgccgc gccggttgct
     9901 gaaatccggg ctgaatctca gctttttgtg acacctgctc cggtctgtga tcgcctggtg
     9961 atgctggcgg agatcagcaa ccgggatcac attctggaac cctctgccgg caccggggcc
    10021 atactgcggg cgattcggga taccgcgccg gaggccatgt gtgatgcggt ggagagcaac
    10081 agcgggctgg tccagtatct gcgggaaaat tttaacggtg tcagggttca gtgtggcgac
    10141 ttcatggaat ggcagccggt gcagtattac agccgggtta tcatgaatcc gccgttcagc
    10201 cacgggcagg atatccggca tatcctgcgg gccttttccc tgttgcgtcc gggtggcgtg
    10261 ctggttgccg tctgtctgaa cgggccgcgc cagcaggaga agctgttacc gttttctgac
    10321 gtccgcgagg agctgccgcg cggcacgttt gcttatacca gtgctccgac gatgattatt
    10381 cgtctgcgtg cctgatgact ccgggcaggc gggaggataa atgcccgttt gcccgcgcca
    10441 ggaaaagacc gcgcgccggt ggcgctgaaa ggggccgcgc tcacgcgccg cccctttcat
    10501 tacgcggacg ttctgctgtg ggcttctgtc ttttcagacc tgtcatttgc ctgttgctga
    10561 tgatgttgtt cctgctactg ctactgctac tgctactgct actgctactg ctactgctac
    10621 tgctactgtt cttctttttc ctgctgctct gattctgact actgcagtgt tgtcctgtct
    10681 gccgggcgcc ccttcggctc ccggctgcct ggcggcatcc ggtcgtcagg cccgactccg
    10741 acaggcagct gctggcgcac ctgccagtct ccgccgaacc cgctggcgcg gtttcgggct
    10801 gtcgctacgg tccgatgcgt gaaaacccct ggcttgcagc gaccgttccg gtcgcgccgc
    10861 ctctgtcgcg ccgtggcctg cgctcccggc atcccggcaa aaggccgccg ggatgtaagg
    10921 cgcaacgccg tttcgcaaac cgcgcttcac gtcgttgcgg cgggtccctg cccgttgttg
    10981 tgtccggtgc cattcacctg tttaagcccc tgtcgccggg ggctgataca gccacaacct
    11041 gccgcactga ctgtccaggg taaatgaaca gcccacaaaa ttttttaccc aaaacccggt
    11101 aaaaaatttt gcgttctgcc caggacaggt gcgtcaggcc gtggcagtga tgccccttgc
    11161 ggcgaaggtt ctcaaacagg tgatggtgaa taccggaggg aacaaaaggc aggtccccgc
    11221 aaaacgtcaa aaccgcggct cccgaaactc aacagatgat gggggctgaa aacatcaaca
    11281 gaaggagata catcatggca gttcgtggca ttaacaaggt cattcttgtc ggtgtgcgcc
    11341 gttgaagcgc ggtgttttat ggagtgttgc atgaaaagca acatggctta gtggtatagc
    11401 cattgcatac tgtggttagc ctcgcttggt aacaggctcg gagatcctga aaaggagagg
    11461 tgaacatagc atccacagaa aagggtggtg aatacggaaa tcgttataag tatcaccact
    11521 caatgcaaga tgtatatgga taaactgggt ttgttgaagt cgagatctat gtgggaaaca
    11581 ggaaaccaat aacgcagaac gattgaagcg ttattcccgt aatagcacaa gaaatccgcc
    11641 ctacgttgat tgtgaccact ctaacgggcc agctattgat gaaatagtta aacgatcgaa
    11701 agacatccta agtacatcgc atctccacac cgtgaaacta cgggactacc taaactggat
    11761 ttatacccag catggtagcg gagtctccat agtactcaag gtcggcctgt aatgggctac
    11821 ggcagtccgc attatacgga agcagcaaag gtataaatca accggaaggg agatgaaagc
    11881 ctcacagtgc tgcggggaag ggagacagtt gttagataca tcaatctcta agaggagggt
    11941 tagccaatgc tgcctgatga acttgagaaa agattagatg gaattgagga tgcttcaaaa
    12001 aaagggtatc cagtacgtaa tctatacaaa attgcgtgtg agcccggact gtggctgcaa
    12061 gcctacgtga atatacgggc caatgcaggt gcactgactc aaggtgtatc aacagatacc
    12121 attgatggct tctctatcga aagagcagag aagctggcaa aaacattgcg gagtagagaa
    12181 tacgtagcga aacctgtacg cagagttcag atcccaaaaa aggacggaaa aaccagaccg
    12241 ctggggattc caggtggaga cgataaactg gcgcaagaag tagcaagaat tattctggaa
    12301 aggtgaaccg ccccgggaat cctggagact aaactccctg agaaagaggt aaacaggatg
    12361 actaaaaata cacgtttttc ccccgaggtc cgtcaacggg cagttcgtat ggttctggaa
    12421 agtcagggcg aatatgactc tcaatgggcg gcaatttgtt ccattgcccc aaagattggc
    12481 tgtacaccag agactctgcg tgtgtgggtt cgtcagcatg agcgggatac cgggagtggt
    12541 gatggtggac tcaccaccgc tgaacgtcag cgtctgaaag agctggaacg tgaaaatcgt
    12601 gaactgcgcc gcagtaacga tatccttcgc caggcttccg cttattttgc gaaggcggag
    12661 ttcgaccgcc tctggaaaaa ataatgccgc tgctggataa gctgcgtgag cagtacgggg
    12721 tcggaccggt atgcagtga
//
LOCUS       DAZGJW010000129        12692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZGJW010000129 DAZGJW010000000
VERSION     DAZGJW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.129"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..292
                     /locus_tag="ACHKIK_003801"
     CDS             <1..292
                     /locus_tag="ACHKIK_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO9084885.1"
                     /translation="SVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQYRTA
                     SILKNRRVVFNIKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            295..654
                     /locus_tag="ACHKIK_003802"
     CDS             295..654
                     /locus_tag="ACHKIK_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9084886.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(701..1093)
                     /locus_tag="ACHKIK_003803"
     CDS             complement(701..1093)
                     /locus_tag="ACHKIK_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9084887.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            1220..1480
                     /locus_tag="ACHKIK_003804"
     CDS             1220..1480
                     /locus_tag="ACHKIK_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9084888.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANAQAAAGVDS"
     gene            1477..1914
                     /locus_tag="ACHKIK_003805"
     CDS             1477..1914
                     /locus_tag="ACHKIK_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO9084889.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            2001..3011
                     /locus_tag="ACHKIK_003806"
     CDS             2001..3011
                     /locus_tag="ACHKIK_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFO9084890.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNSDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEAYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGGTAS
                     RAVQEIRAAREQWERENGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWC
                     DD"
     gene            3004..3465
                     /locus_tag="ACHKIK_003807"
     CDS             3004..3465
                     /locus_tag="ACHKIK_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO9084891.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKLLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            3499..4224
                     /locus_tag="ACHKIK_003808"
     CDS             3499..4224
                     /locus_tag="ACHKIK_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO9084892.1"
                     /translation="METVFDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMVISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPVREAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKHRDIVRQITDSSRRVVSEK"
     gene            4240..4632
                     /locus_tag="ACHKIK_003809"
     CDS             4240..4632
                     /locus_tag="ACHKIK_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO9084893.1"
                     /translation="MAKEFTPEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQN
                     IICRECRQSEVMQRVLAFYRGNFQEVME"
     gene            4629..4925
                     /locus_tag="ACHKIK_003810"
     CDS             4629..4925
                     /locus_tag="ACHKIK_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO9084894.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKAEEELKREGHTVLTPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVAR"
     gene            4922..5383
                     /locus_tag="ACHKIK_003811"
     CDS             4922..5383
                     /locus_tag="ACHKIK_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HFO9084895.1"
                     /translation="MREVNYEALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIAGLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELDAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRG"
     gene            5361..5717
                     /locus_tag="ACHKIK_003812"
     CDS             5361..5717
                     /locus_tag="ACHKIK_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084896.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRKNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            5768..5980
                     /locus_tag="ACHKIK_003813"
     CDS             5768..5980
                     /locus_tag="ACHKIK_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084897.1"
                     /translation="MLNTQKAINAEKYNEWARKFSEQIFKITGDENAAKNELEPWTPE
                     GNAPNYCWWEVDPVDVANEAMSYHND"
     gene            6014..6196
                     /locus_tag="ACHKIK_003814"
     CDS             6014..6196
                     /locus_tag="ACHKIK_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084898.1"
                     /translation="MRKFKIIIETGIAGGDFEDEFEVDDDATPDEIHDEAKDIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            6189..6365
                     /locus_tag="ACHKIK_003815"
     CDS             6189..6365
                     /locus_tag="ACHKIK_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084899.1"
                     /translation="MADFGSTKYSVSFEEWHELLMEYAELRGGSAADAEAWRDDYEAG
                     KNPVEAYCDEWGDE"
     gene            6362..6859
                     /locus_tag="ACHKIK_003816"
                     /pseudo
     CDS             6362..6859
                     /locus_tag="ACHKIK_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            7085..7303
                     /locus_tag="ACHKIK_003817"
     CDS             7085..7303
                     /locus_tag="ACHKIK_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO9084900.1"
                     /translation="MTTLFRKEYPRKSRATEFLFLIIFIVLMIPIPPLLLVWFIVKII
                     EPVIELYNDVVWASFSTLHNKINPYKES"
     gene            7305..7670
                     /locus_tag="ACHKIK_003818"
     CDS             7305..7670
                     /locus_tag="ACHKIK_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO9084901.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            7667..8011
                     /locus_tag="ACHKIK_003819"
     CDS             7667..8011
                     /locus_tag="ACHKIK_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084902.1"
                     /translation="MTTFTREQLIAHAEETIETQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            complement(8216..8515)
                     /locus_tag="ACHKIK_003820"
     CDS             complement(8216..8515)
                     /locus_tag="ACHKIK_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9084903.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNVIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDSRKVFIVGIYHGGQSYETDFL"
     gene            complement(8521..8778)
                     /locus_tag="ACHKIK_003821"
     CDS             complement(8521..8778)
                     /locus_tag="ACHKIK_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO9084904.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRSGK"
     gene            8914..9186
                     /locus_tag="ACHKIK_003822"
     CDS             8914..9186
                     /locus_tag="ACHKIK_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084905.1"
                     /translation="MAHNTKHHNSDIAPASRQGRCSHTFKYDWYQHDPCTEEQAEWLI
                     HNYRRRGYEFKKALSLDYRHWIIYVRLLYSERPPRPSRTFQQRIWR"
     gene            9188..10234
                     /locus_tag="ACHKIK_003823"
     CDS             9188..10234
                     /locus_tag="ACHKIK_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9084906.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAQQNLTAWMIDVIRHAISGAQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQKPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTHWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
     gene            10247..10606
                     /locus_tag="ACHKIK_003824"
     CDS             10247..10606
                     /locus_tag="ACHKIK_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9084907.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            10615..11145
                     /locus_tag="ACHKIK_003825"
     CDS             10615..11145
                     /locus_tag="ACHKIK_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO9084908.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            11387..11584
                     /locus_tag="ACHKIK_003826"
     CDS             11387..11584
                     /locus_tag="ACHKIK_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO9084909.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEVAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            11720..12433
                     /locus_tag="ACHKIK_003827"
     CDS             11720..12433
                     /locus_tag="ACHKIK_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9084910.1"
                     /translation="MAVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMH
                     YGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGE
                     ATDMFLMMACLSLFETDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHM
                     SESLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRHGYGQAIAEKCGYSSRSYFISVF
                     HRYYGFPPDRYVSRQGLDY"
     gene            12462..12537
                     /locus_tag="ACHKIK_003828"
     tRNA            12462..12537
                     /locus_tag="ACHKIK_003828"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12495..12497,aa:Ile,seq:cat)
     gene            12547..12623
                     /locus_tag="ACHKIK_003829"
     tRNA            12547..12623
                     /locus_tag="ACHKIK_003829"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12581..12583,aa:Arg,seq:tcg)
BASE COUNT         3498 a         2675 c         3457 g         3062 t
ORIGIN      
        1 ctcagttaaa acactcaggg atttttgggc ggagaatcct gatgcagaac aaccgctaag
       61 ggcatgggtg gatgaggcgg caaaagctga ctggaaaagc ccagcagaca ttaaggcaca
      121 ataccgaacg gccagtatat tgaaaaaccg gcgcgtggta ttcaacataa aaggtaatca
      181 ttaccgttta attgttgcaa ttgcgtatca gcgaggatgg gtatttgtta aattcatcgg
      241 aaaccacaag gaatacgatg ctattgatgc tgataccatc gaactggagt aagcatgaac
      301 atcaaaccta ttcgtacaga gcaagattat gaagccgcgt tgcgtgctgt tgaacccatg
      361 ttcgacaatg agcccgaaat tgacacgcct gagggggatt tttttgaggt gatgtgtttg
      421 ctaatagagg aatatgaaaa aaaacattat cccattgagc caccatcccc aattgaagct
      481 ataagatttc gcatggaaca gcaggggctg actgtgaaag atttggaacc cgcaattggg
      541 aaaaaaaatc gggtttatga ggtgttgaat ggtaccagaa gccttacgtt accaatgatt
      601 cgccgtcttc ataatcaatt tggtatcccc ctggaaagcc tggttggatt ataaaatctg
      661 ctagtcattt gcctgatgct cgttccagaa aaggaatgca tcaggcagtt ttgtttttct
      721 gccgcagtaa ctcttcaagt ttccgtttat agaaatcgcg tttttgctcc atatcacgaa
      781 tgatctgctc tgcgtcgctt tgaggtaact catctaaaag cgatatgatt tttcgttgtt
      841 gttctgtaag ttgcggttgg ttgtcattac tggatacagc cattttatcg ccgagagttt
      901 cttcttccat aaagaaccaa tggacgggat gttgtgagag ctctgctaat ttttccagtt
      961 tatccattct tggcatcacg cctttcaacc aaccttgcac ggattggggt tttacaccaa
     1021 gacgtcttcc cagctctgac tggtttatat tcaattcctg caacacctgc tgaaggcgtt
     1081 ttacaaagat catcaccacc cctcgtaaac tagttccgcg atcctacaga aaaaattgat
     1141 aagtggcatt acaaatagaa gttgaaattt aaatttaaat ttgtaattat cggtgtcatc
     1201 gtaaagttcg gagggaaaca tgcaaaaaag tactcaagtg aaaatcctgt caataatgag
     1261 ccaatcagaa ttagggcgtc gtcttggtaa aacaccgcaa accataagtg ggtggtttaa
     1321 aaaacgagtg ccagcggagg aggttatacc agcatgtgag gcgcttgact ggggagtaac
     1381 tccgcatgaa ttgcgccctg ataaataccc taatccaacc gatggtttac ctgttgagta
     1441 tcaggctaac gcacaagcag cggcgggagt tgattcatga aaatcaagca tgagcacatc
     1501 cgcatggcga tgaatgcgtg gctgctttat ccgagggtag ggcgcaaaaa aatcgctgat
     1561 gatatagcga cagcatattt tgagcttgaa atgacttatc caccaatgca tgacacctct
     1621 acgacagagg gtattggatt gaacatacaa aatatttttc gctggcttga aaaggatacg
     1681 cctgatgctg ttgaaaaaat tcaggcacta attccagcta tcctgactgt tcttccgcgt
     1741 gaactgcgtt atcacctcag tatttttgac actgttgagc gccgtgcatt actggcggcc
     1801 caggaagcgt tgagcacggc aattgatgcg catgatgatg cagtccaggc agtttaccgg
     1861 aaagcgtatt tcagcgacgg cgggtcatcc ggcgagtctg ttgtggtgca ttgatattta
     1921 tgccagaccc ctgctgattc tgttgattgg ggaatcacag aatatcacca gaggatggtt
     1981 cgtcacaaga tgaggcaatt atggccgcat taccatacat gcaactgtac atagctgatt
     2041 acctggctga caccatgcat ttgtccgcag aggagcacgg tgcgtatttg ttgctgatgt
     2101 tcaattactg gcaaacaggg aaaccaatac ctaaaaacag gctggcaaaa atttcccgtc
     2161 tgactaacga gcgatgggct gatgtggaac catccttgcg ggagtttttt tgcgataaca
     2221 gcgatgaatg ggtgcatctt cggattgagg aagatctggc atcagtcagg gaaaaattga
     2281 ccaaaaaatc agccgccgga aaagcatctg ttcaggccag aagaagcaga aaggaagcat
     2341 atgttcaaac aaaacaagag agagatttaa caggtgttca aacaaatgtt ggtgttgtgt
     2401 ttgaacatga cgcaaacaca aaagcaacta ataaagatac agatctaaaa gaattaaacc
     2461 ccacacataa cgtgcgtgaa cgcgagagta ttccgaccag tgagtcgcat ggtgcgccgt
     2521 tgcagacagc cgaacctgaa tacctggacg gcctgagcga accgatcggg aaattttcga
     2581 tgactactgt ctggcagccg tcgccggatt ttcgacaacg ggcagcagtg tggggtatgg
     2641 ctctgcctga gccggaattt acacctgctg agcttgccgc attccgggat tactggatgg
     2701 cggaggggaa ggttttcacg caggttcagt gggagcagaa atttgcccgc cacgtgcagc
     2761 acgtcagggc acaggtaaaa ccagtcagca agggggtaag ccatgcagca tcaggtggca
     2821 cggcatcacg ggcagttcag gaaatccggg cagcacgcga acagtgggaa cgtgaaaacg
     2881 gatttatcag caacggaaac ggcctggaag ctgtgggagc ttatggggga ggtgtattcg
     2941 aaccgctgga ctcagaagaa cggggccgca ccttcgaagc tctggattgc ccagattggt
     3001 gcgatgactg aacagcaaat ccgtctggtc tgccgtcagt gcatggaccg ctgccgggcg
     3061 ggtgaaacgt ggcccccgga cctggctgag tttgttgcgc tgatttcgga gagtggggca
     3121 aatccatttg gtcttacggt ggatgccgtg atggaagagt accggcgctg gcgcaatgaa
     3181 tcctggcgat acgacgggag tgataaatac ccgtggccac agcctgtgct gtaccacatc
     3241 tgcctcgaaa tgcgtaccag agggattgag cgccagatga cgcagggtga gttaaaacta
     3301 cttgcagaac ggcaactgac gaaatgggca aagcatgttg gtaacgggat gagtgttccg
     3361 ccagtgcgac gacaactgga aggggcgaaa cacccgcaag ggccaacgcc aattgagcgg
     3421 ctgaaacagg aatacgaacg ccggaaggca gctggtttta tttgaatctg agaaacgatt
     3481 ttgtcggagg aaattttaat ggaaaccgta tttgacgcac tgaaagcact gaaaagagcc
     3541 tcttcacagg tagtggcggc ccgccttgga atcagccgtg aagatgcggt caacgaactg
     3601 tggaaactga agcgccgcgg tgaagcggat aacaaggggt cgatgtggtg gctgattcag
     3661 gctggtgaaa gtgaaccggt gtcaccggta ccgaaagtga cagcgcaaat gctgactgag
     3721 gcgattgaac aacatggccc acaaacggcg gatgagctgg cactgatgtt cgggattacc
     3781 tcccgccggg cgaattcatc gctggccatg gtaatcagca aagggcgtct gattcgcgtg
     3841 aatcagggcg gtaaatttcg ttactgcata ccgggcgctg atttaccggc agagccggaa
     3901 gctgcatccg tagcggaaac cgatggtaaa gcctttcctc agccagcagg tgttgcgtta
     3961 ccagttcggg aagcagaaac acaggaagaa ataaaaacgg aaagtgtggc ggtcacagtg
     4021 cagtcacagc cgtcgttcac cagaaaacat ccggatggtc tgattttacc atcgctgcat
     4081 gtggctaacc gcgagctgcg ccgggcaaaa ggtcaggttc agaagtggga gcgagtctgc
     4141 gccgcgctgc gggagctgaa caagcaccgg gatattgttc gacagattac tgattcttcc
     4201 cgccgtgttg tatcggaaaa gtgattgccg gaggcgctta tggcaaaaga atttacacca
     4261 gaagagcggg aagaagtgaa ggcgcgcatt gtggaattcg tgcgcctgag cggacgagaa
     4321 acttttcgac aactggcaga taaaacgggt gtcagtaaga ccgctattcg tcgtttatct
     4381 ggtgcgcttg cggccagtgg tgatgtctgg ctctctggtt gcggggtatt tccatcagag
     4441 caggcgtatc gcgtatggcg taagacaccg gagaaggctg ctgacccgac actgattcga
     4501 aagttacctg acggagaaat acgtcgttac aacagacggc agaacataat ttgtcgtgag
     4561 tgccgccaga gcgaagttat gcagcgtgtg ctggcgttct atcggggaaa ctttcaggag
     4621 gtgatggagt gagggtgaga gtttatattg ccggtccaat gacgggatat gaaaatttca
     4681 accgtgaggc gtttcacaag gcggaagagg aactgaaacg ggaagggcat accgtcttaa
     4741 ctccggcagt acttccggac gggctgacac agccgcacta catggatatt tgcatggcaa
     4801 tgattcgttg tgtggatgcg atttacatgc tgaatggctg gcagcggtca gcgggcgcta
     4861 aggcagagct ggcactggcg gagaaactgg ggcatgcggt gatttatcag gaggtggctc
     4921 gatgagagag gttaactatg aggcgcttcg tgaggcagca caaaactatc agtcgacgct
     4981 ggcgtggtat caggctaccc cggacagccc aaatgctgaa cgggattgtg atgcggctct
     5041 tgctgcgttt aagcgtcata tccgtcatcg ggaagcggat attatcgctg gtttgctgga
     5101 tggactggaa gaagcaaaat cacaactcaa cgagcagcgt gagtattacg aaggcgttat
     5161 ctctgatggg agcaagcgta ttgctgaact ggatgcgcgg gaagttcaat taccgactcg
     5221 ctacgacctt cgatatggac acccgataaa tgcagatgag cgacatgtca tgatacctaa
     5281 agaaaatggc agttggcttt acctgattga cctagaacac gcattacgcg tcgctggcat
     5341 tcgcatcaaa ggagaggagc atggaaataa aaccagagga tgagttaagc aatatcgttt
     5401 tatttccggt aaaagaggat gaccctcgta atcaggttaa ttttctttat gagccatcgg
     5461 aaagaccata ttgtcatcac gcctctgttc gggttgacga aaaagagcgc caggtccgct
     5521 gtaaaatctg cggtgcggtt gtggagccgt ttgactggat gctctctgtg gcgaaaagag
     5581 aaaccagact ggcagatgat gtaaggctat tgcgccagga ggaacaggaa aggcggaaaa
     5641 atatagaaaa gttaattcag attgaacgta acgcgaaagc gcggatacgc agggcgacaa
     5701 aatccagaac tgaataaata aatttagcgc tgtaaataaa atctaatcct gaactggagg
     5761 tatatttatg ttaaatacac agaaagccat taatgcggaa aaatataacg agtgggcaag
     5821 aaaattctct gagcagattt ttaaaattac tggcgatgag aatgcggcaa aaaatgaatt
     5881 agaaccgtgg acacctgaag gaaacgcacc aaattattgc tggtgggagg ttgatccggt
     5941 tgatgttgca aatgaagcca tgagttacca caacgattaa tgtcaggagg ccgcccgaaa
     6001 gggcggtagt taaatgcgaa agtttaaaat aattattgaa acgggaatag ccggtggaga
     6061 tttcgaggat gaattcgaag tggatgatga tgcgacgcct gatgaaatac atgacgaagc
     6121 aaaagatatt ttctttaact actgcaatta ttcatatcac gaaataaaag acgaagagga
     6181 agaacaaaat ggctgatttt ggttcaacta aatacagcgt cagttttgaa gaatggcatg
     6241 aactgttaat ggaatatgca gagttacgtg gtggcagtgc tgctgatgct gaagcatggc
     6301 gtgatgatta tgaagcagga aaaaatccgg tcgaagcata ttgtgatgag tggggcgatg
     6361 aatgagcgag attgactatc aggcgctgcg tgaagcagca gagaaagcaa ctaaaggatg
     6421 ctacatcgta gggcatacat cgggcaatca gcatgggaat ataacaggag tttttgtttg
     6481 tcaaaaatgg aaaggagagc ccggtggcgt aattgcagaa tgtcatgtta actgcctggt
     6541 tgaaacagat gttcaggctt atgcaaacgc tgaatttatt gctgctttta atccaaatgt
     6601 tgcgctggca ctactggatg aacgggaagc ccaaaacaaa cgaattgcag agctgaaagc
     6661 taatcttgtg gcgctggcgg cggagaatgc ggtaataaag tctgcaattc cagaaccacg
     6721 ggatattgag gacgacaatg acaatatgga tgacgtatct cttgctgaag atttcgggtt
     6781 caatcatgca atagaacgga tgaggagacg gattcctgaa actccggcca ccgacgcttt
     6841 cctggctgaa gtacgggcgg ggttgtttaa cgagctttgc gcggcgtttg tcaggtacga
     6901 aaaagttgca ggactggatg atagcgatac agtaacgctt aaggaagcga cagaagcatt
     6961 gctggattgt gcggaacagc ttatcgcgcc tgaataatta aatttagtta aaaatttaat
     7021 ccttaaccgg agggatttct gcaccctcaa aacatcagga ggccgcctga aagggcggta
     7081 agaaatgact acattattca gaaaagaata tccgagaaaa agtagagcga cagaattttt
     7141 gtttctcatt atatttatcg tgttgatgat accgataccc ccgctattac tggtctggtt
     7201 tatcgtgaaa ataattgagc cagttattga attgtataac gacgtggtgt gggcgtcgtt
     7261 cagcacactg cacaataaaa ttaatccgta taaggaaagc tgatatggca ctgacgaaaa
     7321 aacaacgtgc agagctgcgc atgaagttcg gcggtcgctg tgcttattgc ggctgcgaac
     7381 ttggcgaaaa gtggcatgca gaccatgtaa aaccggtcat tcgttttgat ggaaatatgc
     7441 ttcaccagga acgtgacgat atatccaaca tggttccggc atgccaccca tgcaatctgc
     7501 acaagcattg cagtagtctg gaagattatc ggcgaattat cagtgatggt cgtcgtgaat
     7561 tccttgcgtc cgggaaaggc aaagcgctgg ttcgtatggg attggttgaa atgaaatctg
     7621 acccagttgt gttctggttt gaaaaatatc aagaaggggc tacggcatga cgacttttac
     7681 cagagagcag ttaatagctc acgcagagga gactattgaa acacagagac tgtgcatacc
     7741 gggcacaatc gaccatgaca tcatccgcac atataagatg gatattgctg ttctggaaat
     7801 cgcactggta tcgctggcag cagagccagc cggtaaattg catgaataca aaccagtggg
     7861 atatcagcgt ctggtcgatg agttaaccat gctggtaaag cagttaacct ggcaactgag
     7921 gaaagcgaag ccagactgca aattaccgga taaggcgatg agttatctgg agcggaacgg
     7981 actgataagc gtggaggata ttttacgatg acctggcctg aagcattcac aacggtagga
     8041 attgcgatgg cggtggcgct ggtggtgtat tcgatttgcc gctggggata aatcgccgaa
     8101 aaaagatccc gacataaaca tgagccggga tctttgattt atatagccta cgaatccgcc
     8161 agtaagagag ggggcggacg gttaattcta acaccggaat gatgtgggta aaagtttata
     8221 agaaatcggt ttcataactt tgcccaccat gatagatacc gacaataaag acttttctgc
     8281 tatcaacggc aaaagcaata atcgttctgt ggcggaaatg agttacccgc atcccctggc
     8341 gaatatcatc gcgtttattg ccccgatgcg ggaatgtaga aaacccatca agataatcaa
     8401 gaagcgcatt ggcataattg tcagcaatga cgttccctgc tttctccgtt atatacctgt
     8461 gcaggttgat tatttgttgt tcggcctcag gagtaatgat gacttcatat gtcatgcaga
     8521 ttacttcccg gatcgaatcg cggcgcgaac ctgtgaaatg gagcgtccgt tgtttggatt
     8581 ttcgcggata gaatcaagag agggggcggc tgaatgcgtt aaccacgctt cgattgcttt
     8641 atcgcgctca ttcagtgcgc gaagcccttc acgaatgacc tcgctttctg aagcataggc
     8701 accggaagcc acacgggcgc gcaccatgtc agccatttcg ttagttaatg taatgctgaa
     8761 ttgttgggtt gtacgcatgg taaacctcac ggagtaggat agaacaccat tcgatgatag
     8821 cacgttgcct gttgacgaca acagaaatca gagacaatat tgccgcacgc cagcttgaac
     8881 aactggcacc tgctgcgcca gcagagaaaa ccgatggcgc acaataccaa acatcacaat
     8941 tctgatatcg cccctgccag caggcaaggg cggtgttctc acacattcaa atatgactgg
     9001 tatcagcacg atccctgcac tgaagaacag gccgaatggc tgattcataa ctaccgcaga
     9061 cgtgggtatg agtttaagaa agcccttagc ctcgactacc gtcactggat aatctacgtc
     9121 aggctccttt attccgaacg cccgccgcgt ccgtcccgca cattccagca acgcatctgg
     9181 aggtaacgtg cgggtattac ttcgacctgt tctggtaccg gaactcgggc tggtgatcgt
     9241 taagccgggt cgtgaatcca tgccggtatt ccacaatacc cgggtactgg tggagccgga
     9301 accgaaaagc atgcgtaatc tgccgtccgg ggttgttcct gccgttcgcc agccgctagt
     9361 ggaagacaaa acattgctgc cgtttttcag taacgcacgg gtgattcgtg ctgctggtgg
     9421 tgctggtgca ttgtctgact ggctgttgcg ccatattaaa tcctgccagt ggccacacgg
     9481 cgattatcat cacagcgaaa ccgtcattca ccgttatggt accggcgcaa tggtgttgtg
     9541 ctggcactgc gacaaccagc tgcgcgacca gacatccgaa tcactcgggc agcttgctca
     9601 acaaaatctg acagcctgga tgattgacgt catacgtcac gcaataagtg gtgcacagga
     9661 acgggaatta tctctggctg aattatcctg gtgggcggtc cgcaatcagg tggcggacgc
     9721 gctaccggaa gcggtattac gtcgttcgct ggggttgcgt gcggaaaaaa tccgctcaat
     9781 gtaccgtgaa agcgacatcg taccgggaga gcagaccgcc accagcatac tgaagcagcg
     9841 cacaaaaaat cttgcgccgc tgcctcacgc tcaccagcaa aaaccgccac aggaaaagac
     9901 ggtggtcagc attgccgttg atcctgagtc tccggaatct ttcatgaaac gacctaaacg
     9961 tcgccgctgg gttaacgaga aatacacgca ctgggtgaag acacagccgt gtgcgtgttg
    10021 tggtaagcct gctgacgatc cgcatcacct gattggtcat ggtcaggggg gaatggggac
    10081 aaaatctcac gatattttca cgctaccgct gtgtcgggag catcacaacg agcttcatgc
    10141 ggatccgctg gcgttcgaag aaaagcatgg ttctcaggtt gatttaattt ttcgttttct
    10201 tgatcacgcc tttgcaactg gcgtgcttgg gtaaaagagg tgactgatgc tcatagattt
    10261 ggttttacct tacccgccga cggtgaacac ttactggcga cgccgtggca gcacatattt
    10321 tatctcggag gagggaaagc gttatcgccg ggctgtggcg cttattgttc gccagcagcg
    10381 gctgaaatta agcctgtccg gaaggctggc gataaaggtg attgcagagc caccggataa
    10441 gcgccgccgt gacctggaca acattctgaa agcaccgctg gatgcgctga cgcatgcgga
    10501 agtgctcatt gatgacgagc agtttgatga aatcaatatt gtgcgcggtc agccagtatc
    10561 tggtggacgg ctgggtgtga agatttacaa aattgagagt gagtgagcgt aaatatgata
    10621 tatccggaaa ttacaggcaa aagcggcgaa catttacgcc tgaacacgct ggaagcagtc
    10681 tggatccagg ggaaattacg gatgtggggg cggtggtcgt atatcggtgg gggtaaatcc
    10741 ggaaatatgt ttaaccggtt actggtttcg aaaaagctga cgaaaacagc agttaatgag
    10801 gttttacgca gcatgaagaa atccgggctg gaaaaaccgg aacttgaggc attttttcgg
    10861 gatatgacca gagggaagca gaagagctgg ttgtcacatt gtacagacac agaggcgttg
    10921 attattgatc gcgttatcag tgaggtgctt ggggaatatc ccgggctaat caatattctc
    10981 cggcaaaggt acgaaggacg gggaatgagt aagagaaaaa tggcagaatg tttaaatcgt
    11041 actcacccgg aatggtgttt cagcacatgt gagaaacgta ttgcaggttg gttagccgtg
    11101 gctgaacaca tgctttatgt acctatgcac gattcatttc gataaaaaaa gcttgctttt
    11161 ttacgcagaa acagcttgaa ttcctgtaag cttcgcaaag ctgtatcgcg aggcgaaatg
    11221 caagtttttt cgcacaagga agccaccgga aggtggtttt tttgtgtccg taatatacag
    11281 cagcgcaata aattcgctgg tggttattaa taccgttctt tcagcttgct ggctttttcg
    11341 acaagagtta ttggtgtgtc acgttaaccg aaaaagggaa aaagacatgc tgaaacagca
    11401 ggatatgacc gaaaccgcca gagtggtgtt taatgaatta agcgttaccg aaccggcgac
    11461 agtcggggag gttgcgcaga atacttacct ttcacgcgaa cgctgccagt taatactgac
    11521 ccagcttgtt atggcgggtc tggcagacta tcagtgcggt tgttacagac gccttcagtc
    11581 ctgaaggctt tttatttgtg gtgaatgggc ggctggtggg gggggcgaca cctgtcagtc
    11641 ctttgcttat gtgttgatga taatttacct tttggggcta taattgagct aaccaattgc
    11701 taatgaaagt aaaattataa tggctgttgt ctgttcagtt atcatggttt gctccccaat
    11761 taatattttt cttgaaaagg atacgttgtc acttaagcca ggctcagtcg ttctggccac
    11821 caaatgcatc agggagcttt tccttatgca ttatggcaaa gttaaaattg tcgatataag
    11881 cgaatccgtc gtaagtcaat atctggaaag tcagcataag ctgacgagga ctcgtctgac
    11941 tgacattccg ctttacctgt tgctggaacc caacaatcct gcgttggctg cggctttaat
    12001 taccagccag ggattttccg gagaggccac ggatatgttt cttatgatgg cctgcctgtc
    12061 tctgtttgaa acagatgaac ggatgtcatt gtttttaagt ggatgtttat ccagcataag
    12121 tgccaaagtc agggcgataa ttcagacaga tatatcagca agctggacgc ttggtgcgat
    12181 tgctctacag ttgcatatga gtgagagttt gttaaagaca aaactgaaaa atgaaggggg
    12241 catgttcagt cgcttgttgc tggaagagcg gatgcgtgtt gctgtaaata tgttatgttc
    12301 ccggcatgga tatggacagg ctatagcaga aaaatgcggt tattcaagca ggtcctactt
    12361 tatttctgta tttcaccgct attatggctt cccgccagac agatatgtat ccaggcaagg
    12421 gcttgattat tgattttcat ctgattatta ttttttggct cggcccttta gctcagtggt
    12481 gagagcgagc gactcataat cgccaggtcg ctggttcaaa tccagcaagg gccaccatat
    12541 tacataccgc tattagctca tcgggacaga gcgccagcct tcgaagctgg ctgcgcgggg
    12601 gtcaagtccc cgatggcggt ccattatcag catcatgcgt tgttagctca gtcggacaga
    12661 gcaattgcct tctaagcaat cggtcagtgg tt
//
LOCUS       DAZGJW010000130        12451 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZGJW010000130 DAZGJW010000000
VERSION     DAZGJW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.130"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..423
                     /locus_tag="ACHKIK_003830"
     CDS             <1..423
                     /locus_tag="ACHKIK_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFO9084911.1"
                     /translation="RSAGGLAGRLGGGALWAGAMAAPVLLDGSASATDKGEAVGSLAG
                     SIAGGALGAAAGPVGIAIGSTVGSYLGNYLGGWLTEAWQKLRGSDDESSGQAVQKASA
                     RVELVAPEGWQARSIDIDDTSGHGLDVNVWNGGNYGLW"
     gene            410..1759
                     /locus_tag="ACHKIK_003831"
     CDS             410..1759
                     /locus_tag="ACHKIK_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HFO9084912.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLAEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGKVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAFSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIVDPLFIPGGHSVEVISEQQG"
     gene            1743..2867
                     /locus_tag="ACHKIK_003832"
     CDS             1743..2867
                     /locus_tag="ACHKIK_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO9084913.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSVR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            2857..3471
                     /locus_tag="ACHKIK_003833"
     CDS             2857..3471
                     /locus_tag="ACHKIK_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO9084914.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNVTVDKNLHVKGNV
                     SIDGTGRSQGTFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            3464..3901
                     /locus_tag="ACHKIK_003834"
     CDS             3464..3901
                     /locus_tag="ACHKIK_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO9084915.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPVGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHARLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            3901..4983
                     /locus_tag="ACHKIK_003835"
     CDS             3901..4983
                     /locus_tag="ACHKIK_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO9084916.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            4974..5534
                     /locus_tag="ACHKIK_003836"
     CDS             4974..5534
                     /locus_tag="ACHKIK_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO9084917.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEENI"
     gene            5534..6445
                     /locus_tag="ACHKIK_003837"
     CDS             5534..6445
                     /locus_tag="ACHKIK_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9084918.1"
                     /translation="MFHVDNNSGVANMPALAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGALATKNSLGPGDVNALAKDQNLADLENAGTARNNLDVYSKSEGDNRYLRR
                     EQNGADIPDKGAFIDNVGLRETVNKAADALPSGGTAVAANRLATPRNINGVPFDGTLD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEMSWNYSAPSGCMLSGINVQETGSR
                     SADNIGGVYYRPVQIYINNAWRTVSSV"
     gene            complement(6480..7001)
                     /locus_tag="ACHKIK_003838"
     CDS             complement(6480..7001)
                     /locus_tag="ACHKIK_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO9084919.1"
                     /translation="MELINVKRYYPEHKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASE
                     GHYQAIPWPEKPE"
     gene            complement(7081..7284)
                     /locus_tag="ACHKIK_003839"
     CDS             complement(7081..7284)
                     /locus_tag="ACHKIK_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9084920.1"
                     /translation="MADSRYVQSIRRGSRSTIGMQYNIFEVPDGCVLTGLDVAGDGNA
                     TVTAYYRPVQFLIDGSWKTASSA"
     gene            7313..7492
                     /locus_tag="ACHKIK_003840"
     CDS             7313..7492
                     /locus_tag="ACHKIK_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084921.1"
                     /translation="MSCVPSNGTPLIFLGVASLFAATAAPFDGRAFAALFTVSRKPRF
                     QGCRISALKGLSTSF"
     gene            7506..8066
                     /locus_tag="ACHKIK_003841"
     CDS             7506..8066
                     /locus_tag="ACHKIK_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO9084922.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
     gene            8166..10205
                     /locus_tag="ACHKIK_003842"
     CDS             8166..10205
                     /locus_tag="ACHKIK_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO9084923.1"
                     /translation="MTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMTFAD
                     CLISFNTRDCLWVRPVSGDPSVEITGAGIGAVIPLSADVAGTAEPSDWDNAETHTRPS
                     GNETASSSPSWYYVVVLAGQSNGMAYGEGLPLPETYDRPEPRIMQLARRSTVTPGGKA
                     CQYNDIILADHCLHDVQDMSGKNHPKADVAKGQYGTVGQGLHIAKKLLPFIPADAGIL
                     LVPCCRGGSAFTAGADGTYSDSTGASEDSARWGVDKPLYKDLISRTKAALAKNPKNRL
                     LAVVWMQGEFDIDAKPTEHSALFLAMVEKFRADLAEQAEQCTGGSAAGVPWICGDTTY
                     FWKQKNEPAYQAIYGGYKNKTDKNIHFVPLMTDENDANVPTNNPAEDPDIESIGYYGS
                     TWRNSAATWTSADRASHFSSWARRGIISDRLATAVLTHAGRTTVKADVPSSETEAPVP
                     SPSETEAVTTTLLSYRVSESEGNLKAQGWEPAGGKAEIISDAGGTGGKAMKLTKETGK
                     SSWYLDHDAGTGAELLKNGGLISCRFKVPGDLVANQYVMGLYWPVSSLPQGVTLTGDA
                     GNNLLASFYIQTDAKDLNVMYHNAKVATNNQKLGTFGAFDNEWHTLAFRFAGNNSLQV
                     IPVIDGQDGAAFTLTQSPVGTFPVDKLRVTDITKNATYPVLIDSIVVEVKKAVTE"
     gene            10352..10534
                     /locus_tag="ACHKIK_003843"
     CDS             10352..10534
                     /locus_tag="ACHKIK_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO9084924.1"
                     /translation="MTFVHTMLLYFCAVVSALYLVSGGYKVIRNYIRRKIDDAAAEKL
                     SKTAQAPSSPNDPTPL"
     gene            10570..10815
                     /locus_tag="ACHKIK_003844"
     CDS             10570..10815
                     /locus_tag="ACHKIK_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO9084925.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGRSPAENSTENDPQPVEQPEPVQPGTDGTMM"
     gene            complement(10854..11318)
                     /locus_tag="ACHKIK_003845"
     CDS             complement(10854..11318)
                     /locus_tag="ACHKIK_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084926.1"
                     /translation="MIQGGDYLLKENEELGGENDHKMIILLTFYTVKVGSRVYVVCFK
                     STGYYIKVKPSIAGTMLGQSVKTCINALLRARGVSYDIPRSPVKQRCIKRRSLSWMKE
                     PVCPACKTPLQLIYRRSRSQNGKTYKPKNASCFRMLICPACSPRRENTQNVH"
     gene            11433..11633
                     /locus_tag="ACHKIK_003846"
     CDS             11433..11633
                     /locus_tag="ACHKIK_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9084927.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSPSATEHP
                     TYTRKSCRGEQKASHIPNHQHA"
     gene            11587..12324
                     /locus_tag="ACHKIK_003847"
     CDS             11587..12324
                     /locus_tag="ACHKIK_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFO9084928.1"
                     /translation="MGNRKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARL
                     VIVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNSGARVVTGTQNREYMELN
                     RLWLHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHESIFYELDGEWYHEICRNAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLNKRAR
                     KRLNTKLFKVQPYPKPQTVKTGLKESE"
BASE COUNT         3066 a         3002 c         3449 g         2934 t
ORIGIN      
        1 cgcagtgccg gtggactggc cggccggctg ggtggcggtg ccctgtgggc tggcgcgatg
       61 gcagccccgg ttctgctgga tggcagcgcc agtgccacag acaagggaga agcggtcggc
      121 tctcttgcag gcagtattgc cggtggtgcg ctgggggcgg cagccggacc tgtggggatt
      181 gccattggtt ctacggtggg cagctatctc ggtaactatc tggggggatg gctgactgaa
      241 gcctggcaga aattacgggg cagtgatgat gaaagcagtg gacaggcggt acaaaaagcc
      301 tctgcccgtg tggagctggt tgccccggaa gggtggcagg ctcgcagtat tgatattgat
      361 gacacgtccg gtcacggact ggatgtgaat gtctggaacg gagggaatta tggcctctgg
      421 tgacggtcgt ggtgcgttcc gtggtgtgcc gttcctcgtc tggcgtgagc agcgcgaacg
      481 tggcgggcga aacattgtcc gtcgggaata tccgttacgg gaaaccggtg gtgcagacga
      541 tctgggacct aaactggctg aatttacgtt cagtgtactt gtgatggggg atgatgttca
      601 gacacagcga aatcgtctgc gtgatgccct tcgtgctccc ggtgccgggg agctgcttca
      661 cccggattac ggcacgttaa atgtgctgat aaacagcttt gaaagtcgtt ataacgctgc
      721 tgaacagggc gtggttgagt tcaccattaa tgtcacgccg gtaagtgatg ataccgcacc
      781 tgctgtcaca caggacacgg ccgccattct ggagcagaag agtaccaccg cgctcgggaa
      841 ggtttttgaa accctggaag cgggctggac ggtgatttct gacggaatgc acgatgtcca
      901 ggcaatgacc gaaaccatca gcgataaggt gtccgccctg gaaaatgccg tttccggcat
      961 ggggattgta caggatatca gtgcctttac tgccacgttc acggcgctga aagggaatgc
     1021 cacggcatta ctgacagcac cttcccgcat ggcctcatct tttgcggggc ttttcagtgc
     1081 cctgatcacc ctgccatcgc tgccgtcact gtcttcagga ggcctgaata cccggcccgg
     1141 tggcagtatc ggccggacga catccgcctt ctctcaggga atgccgcaac tgtacaggac
     1201 attatcttcc ctgcgttacg tgctggatga gcaggataat ccgcagactc tcatcggtct
     1261 gacaccggca gcacaaaaga atattcgtct gatacgggcg gtaatgcaga gtgctgccgt
     1321 ggtggcccag gcacagaccg tgggaaaact gctggatcag gttctcagtc aggaaaccct
     1381 gccggacagt gacgcagccc accgtacctg gcctgtctgg ctggaaagtt cagttgatct
     1441 tcagcgcatt aaccgtgact taagcgaagc gctcgaacgg caggtgatga cactgtccgg
     1501 gcaggggtat accgccacgg cgctgacgct tcgtgatgcc agactggcac tgacagagga
     1561 tctgaataca cggggagttc agctacccgg tgcaacagta gtgactgtac gtaccaccga
     1621 gcctgcactg gtgaccctgt accgtgccac cgggaacagt accggctggc aacgttttgt
     1681 gcgccgtaac ggtattgttg atccgctgtt cattcccgga ggccattcag tggaggtgat
     1741 tagtgagcag cagggttgaa ctgtatctgg gcggtgagat tttttccggc tggctgacgg
     1801 tgagtgttcg tcgctctctt gaacatctgg cgggctcctt tgaactgggg gtaatgatgc
     1861 ccggtgtacg ccttccgtca tccgtccgtg ccggtcagtc tctggaattg cgcattgacg
     1921 gtcagcctgt gatcactggc tggctggatc aggtccggca gcgcatcagc gccacgcgtt
     1981 ttcagatcac gctcagcgga cgggataaaa ccggtgacct ggtggactgt tcagccattc
     2041 atccgggcag ccagtggagg aaccgcacgc tggagcacat tgcttcagat ttgtgtgctc
     2101 cgttcggggt cacggtgcgc tggcaggtaa atgatgcaac ggcagcccgg cccttttcca
     2161 ccttcacact ggaaaactca gaaaccgtgg cagatgcgct gacacgggcc gcgagacaca
     2221 gaggcgtcct ggtaaccagt aatgccgccg gtgaactggt tttcactcag gccggcagtc
     2281 agcgtggcga cacgctgacg ctgggcgaaa atctgctgga tttggatcac aacgtggatc
     2341 accgtctgcg ccacagtgaa taccgtgtac gggggcacgg gcgtggtggt ggtcatgccg
     2401 gggatgcact gacagccgga acgctggccg cacccgttgg tacggtgaca gacagtgcca
     2461 tccaccgtta cagaccgaaa attgtgctgg cggatcatgc tgttgatgca gacggtgcac
     2521 gccagagggc tgtccgggaa atgcgccggg cggttgcccg ctctgtgcgc ctgacagcca
     2581 ccgtgcggca ctggtttcgg gagaacggcc agttgtggga tatcaacctg ctgacggctg
     2641 tcacggctcc ccgcaccgga gtggaagagc gtgatcttct tgtctgtcag gtggagtttt
     2701 cgctggatgc aaatcacggc gaaaccaccc gtctgattct ggcaccccgt gacggcttta
     2761 ttgttccggc agagccggga aacagcggaa gcggaaatgc gggtgacgtg gacgccttcg
     2821 tgcgggcaca gatgaaaaaa caggggatta aattcaatga tgaatgacga agtcatcagc
     2881 cgccttctgg cccccgtgat gcggggtgtt cgtctgctgt tcgggcgtgg tgtactgacc
     2941 ggcacaacgg acacgctgaa aatccagaat gtgcaaatca ccggtatgga tggtgaaacc
     3001 tttgatgacg ttgaacgccc ccagcagtac gggcaaatca gcgtccccct gcctggtgcg
     3061 gaagtttttc tggcctgtgc tggcggacaa cgggatcagg ccgtggtgct tgtggtggaa
     3121 gaccgccgca gtcgcccgac cggacttacc gccggagata ccggcgtgta tcaccatgag
     3181 gggcaccgta tacgtctgac aaagaacggc cggatcattg tgacctgtaa gacgctggag
     3241 atttacgccg atgaaggtgt tcaggtggat acaccggagg ctcactttac cggtaatgtc
     3301 acagtggata agaacctgca tgtcaaaggc aatgtgtcca ttgacggcac cggaagatca
     3361 caggggacgt tcacgatgtc cgaagcggtt attgccggga tcacctattc aggtcatgtg
     3421 catcacgata acggtgaagg cagcaaaacg ggaggaccag agaatggctg atattgctgt
     3481 cgtctgggat cagggttgcg gttccctgca actgaacggc gcagatcttc tgacggataa
     3541 cagcctgctg actgcggtca ttatttcact gtttacggac aggcgggcgc tggattccga
     3601 tgaaatccct gacggcaccc gtgaccgtcg gggatggtgg ggagacagtt tccgggagcg
     3661 ccccgttggc tcccgtctct ggctgttaag ccgtgaaaag acgctgtcct ccgtggtcag
     3721 ccgtgcacag gcctatgctg atgaagcgct ggcgtggctg cataaaagcg gtgctgccac
     3781 atccgtggta tgtcatgcca tgcgtgtggg gcatgctcgc ctttcgcttt ccgtgaaaat
     3841 caccctgccg gacggaagca gacatccgat gattttttat gctgatatga agggggaatg
     3901 atgccttatc agcccttacc actggcgcaa ctgatcacac agacacaaca ggatatcagc
     3961 cagcgcctgc ccggttcgca gccgggcgtg aatgaaacca ccctgaatgc cattgcgtat
     4021 gcactggcgg gtctgtcagc acaggagcat gaacatctgg cctggatctc ccggcagata
     4081 attccgacag aagctgatga agccgaactc ctgaaacact gcgcattctg gggtgtcatc
     4141 cgtaaaccgg cttcccgcgc tgacggaccg gtacaactga tgctgaccac ggatgcaggg
     4201 atcacggaag gcgtactcct tcagcgaagc gatggtgttg tgtaccgcat caccggctct
     4261 gcgaccggaa aagccggaac actgaatgtt aatgtggagg cggaaagtgc ggggcgcgct
     4321 ggaaataccc cgaccggaac ccgcctgtcc tttatcacgc cacaggcggg catcaaccag
     4381 acagccacgg tcaccggcac gggactcacc ggtggtgcgg atgtggaaac ggtgccggag
     4441 ttgctgtcca ggctggtatt ccgggtacag aacccgccat cagggggaac acagtatgat
     4501 tttgaacgct gggcacggga agtaccgggc gtgacgcggg catggtgtaa gcctgaatgg
     4561 cctgaggcgg gtagtgttgg tgtgactttt gttcaggata ataaccctga cattttcccc
     4621 ggagaaggtg atgtgaagcg ggtggcggat tatatccgca gtcatgatga tccggcgacg
     4681 ggccagcccg ttggtcagcc acttgggccg acaatcagcg tgtttaagct gaccaataag
     4741 ccggtggcgt ttgagatcag gattgtaccc aaaacgccgg aaaatcaggc tgccgtaaaa
     4801 caggcattaa cggacctgct ttataacgaa tcgcggccgg gtggacttgt attgccttca
     4861 tcattctggc gggctgttgc aggggtgaaa ggactggagg attttgaagt tcgcagcccg
     4921 ctgaagtccg tgatggccgg agatacagag ttgctgaccg tgggggaaat cacatggctg
     4981 taaccctgac cccgcatcag cgtgccctgt tgcagttgct gcctgacggg ctggcatggg
     5041 ataagcggcc gtcatccgtt cttgcggctt tgtgcctggg cctcagtcat tccacggagc
     5101 gtgtttcctg gaccggtaac cagatgctgg cagaacgttt tcctgattca tcccgtctgc
     5161 tgctggaaga ctgggagcgt tatctggggt taccggaatg tgatatgacc ggcgcaacca
     5221 ttcaggagcg tcagcgttat gccgggaata aataccggat gaaaccctct cttaaccgtg
     5281 aattttatat ccggtttgcg gcagagtttg gttatgaaat agatattcag ccatcaccgg
     5341 attcacagtg ggtcagtatt gtcacgatta acagtgaaac cggctaccgg aatatgaatg
     5401 tgctggatga tattctcacg ccgctgcgta tttatgaagg cggtgcgctg gaatgtattc
     5461 tgaaccgtta taagcctgca tggcagacgt ttatttacgt atatgcaaac agtcacgaag
     5521 aggagaatat ttaatgtttc atgttgataa taattccggc gtggcgaata tgcctgcgct
     5581 ggcaccggcg cagagtaata ccaccacctg gtttaccgaa ggtgacggac aaaaaggtat
     5641 cagctggatt ggtcaggact ggctgaatat tctccaggcc gaactgctga atattctggc
     5701 tgaagccagt attcagccgg ataaggcgca gttaaaccag cttacgctgt ccattaaagc
     5761 cattatcgct gcgaatgcct tttcccggaa aaacaacctg aaagaaattg ctgatgccgg
     5821 tgcggaagcc cagcgtcttg cccgtggtta tcttggtctt ggggcgcttg ccacaaaaaa
     5881 cagtcttggt cccggtgacg ttaatgctct ggcgaaggat cagaaccttg ccgacctgga
     5941 gaatgcggga accgcccgta ataatctgga tgtttacagc aaaagcgaag gtgataaccg
     6001 ttacctgcgc agggagcaga acggcgcaga cattccggat aaaggcgctt ttatcgataa
     6061 cgttggttta cgggaaacgg taaataaggc tgctgatgcc ctgccatcgg gtggaaccgc
     6121 cgtggcagca aacagactcg ccaccccaag aaatattaat ggtgttcctt ttgacggaac
     6181 gctggatatc aacatcacgt caggaatgac gcagtcaacc gcagatggcc ggtatgtaca
     6241 gaatgttcag cttggagcac agagctatca ttcacccgga ggcaatgaaa tgtcatggaa
     6301 ttacagcgca ccttccggtt gtatgctttc cggaattaac gtgcaggaaa ccggctcccg
     6361 gtctgcggac aatatcggtg gggtctatta tcgcccggtt cagatttata ttaataatgc
     6421 ctggagaacg gtaagttcag tgtaaaccac agaaaagggt gctgaatgca ccctctgaat
     6481 tattccggtt tttccggcca gggaatcgcc tggtaatgcc cctcactggc aatatcatta
     6541 aagtgcattt ctttaagctg tcggatatac gccatccagc gcgtcagctg taatttattt
     6601 tcatcgctaa tcatgtccag ttttaattcc gttttccagt catccacagt ttcgttggct
     6661 tcctggagca gcgatgagcg gcgcttttca gcaaccgtct tccagtttac cggcaccttt
     6721 gatatcaggc catcagaaaa ctgccagtta ccgtcaatat tcacaccttc cggcagttcg
     6781 tccacttcaa caacagtaaa acctgccgga tataaagcag agacatcttc agcaacagag
     6841 cagataatac ccgttacagg cgaaatacag agtttgtatt tctttgtgaa aagcgggata
     6901 gattcataaa aatcccttcc gtcctcactc tggaaatact gaacatcctc accatagggt
     6961 ttgtgttccg ggtaatatcg ttttacgtta ataagctcca ttatttcacc ttgtttttgt
     7021 tgaaaagaca aaatcatcat atatttattc agataatctg ttttttgaac tactgcactt
     7081 ttatgcgctg gaagctgttt tccacgaacc atctatcaga aactgaacag gtcgataata
     7141 tgcggttacc gttgcattac catcaccagc gacatcaagc cctgtaagta cgcatccgtc
     7201 aggaacctca aaaatattat attgcattcc aatcgttgac cttgaacctc ttcgaatgct
     7261 ttgaacataa cgtgagtcag ccacagactg cgtcatcccc gatgtaatat ttatatcctg
     7321 cgttccgtca aatgggacac cattaatatt tcttggagtg gcgagtttat tcgctgcaac
     7381 agccgctccg ttcgatggca gggcattcgc ggccctgttt acagtatccc gtaaaccgag
     7441 gtttcaggga tgccgtatat ctgctttaaa aggcctttcc acctcttttt aaggaaggta
     7501 aatacatgct gattggctat atacgcgtat caacaaatga ccagaacaca ggtttacaaa
     7561 gaaatgcgct ggaatgtgca ggatgtgagc agatttttga agataaaatg agtggtaaaa
     7621 cgacacagcg gccgggactg aagcgggcac tgaagaaact aaaacccggt gacacactga
     7681 tggtctggaa actggacagg ctggggcgca gtatgcgcca ccttgttgcg ctgacagatg
     7741 aattacagca acaggggata aattttcgta gtcttactga caacattgat acgtcaaccc
     7801 ccatggggcg cttctttttt cacataatgg gtgcgctggc agaaatggag cgtgaattaa
     7861 ttgtcgaacg gacacgggct ggtcttgcag ctgcccgtga aaggggacga accggcggca
     7921 gaccccgtcg tctcacgcag gaacaatacg agcaggtcgg gcgtttactg gccgggggtg
     7981 tcagtcgaag acaggtggca cttatttttg atatcggcct ttcaaccctt tatcgttatt
     8041 atcctgcgag cgtttcggaa aaataagacg ctccctttgt tgggccttta ctgaattaac
     8101 gcaaaatggc gtttatcggt ctgtggatat gataattcag tttattcagg aacaggctga
     8161 ttattatgac gtccaaatgg gttcaactat catcaatgcc cggaaacttt actgttaaag
     8221 tttccggtgg tacagcggca tttcttgagg ctccctttcc tccggccgaa acaaaaggag
     8281 gaatgacatt tgctgactgt ctcatcagtt ttaatacacg ggattgcctg tgggtaaggc
     8341 cagtatccgg tgatccgagt gtggaaatta ccggagcggg tattggtgcg gtcattccgt
     8401 taagtgctga tgttgccggt actgctgaac cgtcagactg ggataatgct gaaacacata
     8461 cccgtccgtc aggaaatgaa actgcttcca gctccccttc ctggtattac gtggtggttc
     8521 ttgctggtca gtcaaacggt atggcttacg gtgaaggtct gccactgccg gagacatatg
     8581 accgccccga accgcgtatt atgcagttag cccgtcgcag tacagtcacg ccgggaggta
     8641 aagcctgtca gtataacgac atcattctgg ccgaccactg tctgcatgat gtgcaggata
     8701 tgagtggaaa aaatcacccg aaagcggatg tggctaaagg gcaatatggc accgtgggtc
     8761 aggggcttca cattgccaaa aaactgctgc cgtttattcc ggcagatgcg gggattctgc
     8821 tggttccctg ctgccgtggt ggttctgcat tcacggcggg tgctgacggc acgtacagtg
     8881 acagcacggg tgcttcagag gactcagccc gctggggtgt ggataagccg ctgtataagg
     8941 acttaatcag ccggacaaaa gcggcactgg cgaagaaccc gaaaaaccgc ctgcttgctg
     9001 tggtgtggat gcagggcgag tttgatattg atgcgaagcc gacggagcat tccgcgctgt
     9061 ttctggcgat ggtggaaaaa ttccgcgcag acctggctga acaggcggaa cagtgtaccg
     9121 gtggcagtgc tgctggcgtt ccgtggattt gtggcgatac cacgtatttc tggaaacaga
     9181 aaaatgaacc ggcataccag gcaatctatg gcggctacaa aaacaaaaca gataaaaaca
     9241 ttcatttcgt tccattaatg acagatgaaa atgacgcgaa tgtacccacc aacaatccgg
     9301 cagaagaccc ggatattgaa tccattggtt attacggctc aacgtggcgt aacagtgccg
     9361 ccacctggac atctgcggac cgggccagcc atttcagttc atgggcacgt cgtgggatta
     9421 tttccgaccg tctggccacg gcagttctga ctcatgcggg gcgaaccaca gtaaaagccg
     9481 atgttccatc ttctgaaact gaagcaccag tgccgtcacc ttcagaaact gaagcagtaa
     9541 ccacaacact gctgtcttat cgtgtcagtg agtcagaagg aaatctgaaa gcccagggct
     9601 gggagccggc tggcggtaaa gctgaaatta tcagtgatgc aggaggcaca ggtggtaagg
     9661 caatgaaact gaccaaggaa acaggtaaat catcttggta tctggatcat gatgccggta
     9721 ctggtgctga actgctgaaa aatggtggtt taatcagttg tcgttttaaa gtacctggcg
     9781 acctggtggc aaaccagtat gtcatggggc tttactggcc ggtttcctct ctgccgcagg
     9841 gtgtcaccct gacaggggat gcagggaata atctgctggc atcgttctac atccagacag
     9901 acgcaaaaga cctgaatgtg atgtaccaca atgcaaaagt ggcaacaaat aaccagaaac
     9961 tgggaacctt tggtgcattt gataatgaat ggcatacgct ggccttccgt tttgcaggaa
    10021 ataacagcct tcaggttatt ccggttattg atggtcagga tggtgccgcg tttaccctga
    10081 cgcagtcacc agtaggaact ttcccggtgg acaaactccg cgtaacggat atcacaaaaa
    10141 atgcaactta cccggtgctg attgacagca ttgtggtgga agtgaaaaaa gcggtaacag
    10201 aataaaaaaa tcccgccggt catgatatgg ctggcgggag ctacctaacc cataaaggaa
    10261 aataaaaata ctcccgaagg agtggttatc agtcgcttcg atgtttttac ccgataacga
    10321 attatgtagt caatatcaaa ggtaaggtta tatgacattc gttcatacaa tgctgcttta
    10381 tttctgcgct gtggtcagtg cgttatatct ggtgagcggt ggttataagg tcatcagaaa
    10441 ttatattcgc cgtaaaattg atgatgcggc agctgaaaaa ctcagtaaaa cagcacaggc
    10501 accgtcatcc ccaaacgacc cgaccccgct ctgataacgg ggcaatatac ctgcaaaagg
    10561 agaatattca tgccagagat taaaggcacg gttactgaag agcttgtcaa acaggcactt
    10621 tattctgaag aagtgaaccg cgtgctgaag gcgcaggttc gcaaggattt tgaggcacaa
    10681 atcgacgcat atgttgatga agtgctggcc cgaatggttg gccgttctcc ggctgaaaac
    10741 agcacggaaa atgatcctca acccgtagag caaccagagc cggttcagcc cggaactgac
    10801 ggtactatga tgtaacaata ccgaccggca ggtttccctg ccgggtgagt ttgttaatgc
    10861 acgttttgtg tattttccct cctgggagaa caggcaggac atatcagcat cctgaaacag
    10921 gaagcatttt tgggtttgta tgtcttgcca ttttgagatc gagaacggcg atagatgagc
    10981 tgtaagggag tcttacaggc agggcaaaca ggctctttca tccatgataa agatctcctt
    11041 tttatgcatc tctgctttac aggagaccgg ggaatatcat aggatacgcc acgagctcga
    11101 agtaaagcat tgatgcaagt ttttacagat tgacccagca tagttccagc tatgctgggt
    11161 ttcactttta tgtagtatcc tgttgatttg aaacatacta catatacgcg tgatcctacc
    11221 ttcactgtat aaaaagtcaa taaaatgatc attttatgat cgttttcacc acctaactct
    11281 tcgttttctt taagtaaata atcacctccc tgaatcattg ttgatgactt ttcaatctat
    11341 ttgttgtatc aattaaacaa atcgaatcga tcgtttttgt cgatcgattg catgagaaag
    11401 gcatcaacaa caaagcagga ggttatgcgg tcatgtatcg aaacatacgg tgccgacact
    11461 gcaataagct actggcacgg gccagtttca gttatctgga agtaaaatgt ccgcgctgta
    11521 aaaccctcaa tcaaatcaca tctccgagcg ccacagagca ccccacatac acaaggaaat
    11581 cctgtcgtgg ggaacagaaa gcaagtcaca tcccgaatca tcagcacgcc tgaacttatt
    11641 cgctataacg acaatatcgt tggttatggc tcccgtgagt tgcgggttga gacaataagt
    11701 tgctggctgg ccagactggt catcgtcaat aaacattaca gccaccgttt cgtaaataac
    11761 tcctaccttc atctgggcat attttcagaa cgggaacttg ttggcgtaat gcaatggggt
    11821 tacgccctta acccaaacag tggtgcacgc gtcgtaacgg gtacgcagaa ccgagaatat
    11881 atggagctta accggctatg gctgcatgac tgtatgccga gaaactccga atcaagggca
    11941 atcagttacg cgctcaagct aatcaggcag ctttatccgc aggtacagtg ggttcagtcg
    12001 tttgcagatg agcgttgtgg ctgtcttggt gtcgtgtatc aggcaagcaa ttttgattat
    12061 gtaggcagcc atgaatcaat attctatgaa cttgatggcg agtggtatca cgagatttgc
    12121 aggaatgcca ttaaacgagg cggacaacga ggtgaacatc tgagggctaa tattgacaga
    12181 gccagtgttc ataagttccg acagtttcgt tacatccggt ttctgaacaa gagggccaga
    12241 aaacgcctca acacaaagct attcaaagtc cagccttacc caaaaccaca aacagttaaa
    12301 actggtttga aagagagtga atgagcgctt aaaaactcat tctaaaactc aaaagaaaag
    12361 cgccgattta gtatcaaata agttgcgtat cggcgttttt cagcggtatc aaatgaaatg
    12421 caaatcggta tcaaaaaaat cgccgcgcta t
//
LOCUS       DAZGJW010000131        12442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZGJW010000131 DAZGJW010000000
VERSION     DAZGJW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.131"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(130..1554)
                     /gene="patD"
                     /locus_tag="ACHKIK_003848"
     CDS             complement(130..1554)
                     /gene="patD"
                     /locus_tag="ACHKIK_003848"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO9084929.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1576..2370)
                     /gene="ydcV"
                     /locus_tag="ACHKIK_003849"
     CDS             complement(1576..2370)
                     /gene="ydcV"
                     /locus_tag="ACHKIK_003849"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9084930.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2360..3301)
                     /gene="ydcU"
                     /locus_tag="ACHKIK_003850"
     CDS             complement(2360..3301)
                     /gene="ydcU"
                     /locus_tag="ACHKIK_003850"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9084931.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPRLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(3302..4315)
                     /gene="ydcT"
                     /locus_tag="ACHKIK_003851"
     CDS             complement(3302..4315)
                     /gene="ydcT"
                     /locus_tag="ACHKIK_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9084932.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(4333..5478)
                     /gene="ydcS"
                     /locus_tag="ACHKIK_003852"
     CDS             complement(4333..5478)
                     /gene="ydcS"
                     /locus_tag="ACHKIK_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO9084933.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(5723..7128)
                     /gene="ydcR"
                     /locus_tag="ACHKIK_003853"
                     /pseudo
     CDS             complement(5723..7128)
                     /gene="ydcR"
                     /locus_tag="ACHKIK_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            complement(7207..7623)
                     /gene="hicB"
                     /locus_tag="ACHKIK_003854"
     CDS             complement(7207..7623)
                     /gene="hicB"
                     /locus_tag="ACHKIK_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO9084934.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(7669..7845)
                     /gene="hicA"
                     /locus_tag="ACHKIK_003855"
     CDS             complement(7669..7845)
                     /gene="hicA"
                     /locus_tag="ACHKIK_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFO9084935.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            8067..8297
                     /gene="yncJ"
                     /locus_tag="ACHKIK_003856"
     CDS             8067..8297
                     /gene="yncJ"
                     /locus_tag="ACHKIK_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO9084936.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(8389..10350)
                     /gene="rlhA"
                     /locus_tag="ACHKIK_003857"
     CDS             complement(8389..10350)
                     /gene="rlhA"
                     /locus_tag="ACHKIK_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO9084937.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFFANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(10423..10959)
                     /gene="sutR"
                     /locus_tag="ACHKIK_003858"
     CDS             complement(10423..10959)
                     /gene="sutR"
                     /locus_tag="ACHKIK_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO9084938.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            11012..12226
                     /gene="ydcO"
                     /locus_tag="ACHKIK_003859"
     CDS             11012..12226
                     /gene="ydcO"
                     /locus_tag="ACHKIK_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO9084939.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            complement(12266..>12442)
                     /locus_tag="ACHKIK_003860"
     CDS             complement(12266..>12442)
                     /locus_tag="ACHKIK_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO9084940.1"
                     /translation="RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     PLKQEPTEMIQATA"
BASE COUNT         3041 a         3343 c         3050 g         3008 t
ORIGIN      
        1 aaatcgccat caaacattta gcctaatttt tacgctagtt attaatgtaa tcattctaat
       61 acttaaccgc cattaaactc agaacctaaa acgatagtgg ccgtcctata acagccacta
      121 acatctaact taatgtttaa ccatgacgtg gcggacgacg gtgtaatcct ccagcccata
      181 aagtgacata tccttgccgt aaccagaaag tttctgccca ccgtgcggca tttcacttac
      241 cagcatgaaa tgggtattga cccaggtaca accatattgc agccgtgcgc tgacgcgatg
      301 cgccctgccc acatctttcg tccataccga agatgcaagt ccgtactggc tgtcattcgc
      361 ccagttcacc acctgttctt cgttgtcgaa gggcgtaaca ctcactactg gaccaaatac
      421 ctctttttgc acgatggcat cgtcctgtaa tgcgccagcc agcagcgtcg gcgcatagta
      481 atagccatta cccttgcgct tttcaccgcc agtgatcact ttgatgtgcc ctgtcgcttt
      541 cgcctcttct actgccttgc tgacgcgttc gagatgcgcc agcgagctta aaggtccaag
      601 ctccgtagac tcgtcatccg gcgcaccaga ttttaacgtt gccaccgcag cacccagttt
      661 ttccaccagc gtatcgtaaa tgcctttttg cgcgtagatc cgacaagccg cagtacaatc
      721 ctgtccagca ttgtaatagc caaatgtacg tacaccttcg accactgctt caatatccgc
      781 atcatcaaaa acaatcactg gcgctttgcc accaagttcc atatgagtac gcttaatgga
      841 cgacgcggta tggctgatga tgtgctcgcc ggtggcgata gagcccgtca gcgacaccat
      901 ccgcactttg ggatgaccgg tcagcggatc acccaccgtt ttgcctctgc caaacagtac
      961 gttaatcacg cctgccggga agatatcttt cgccagctct gccaacttca acgcggtcag
     1021 cggggtaatt tctgatggtt taagcactac gcagttccct gccgccagcg ccggagccag
     1081 tttccacgct gccatcatca gcggataatt ccacggtgcg atagaagcca cgacccccaa
     1141 cggatcgcga cggatcatcg aagtatgacc ttcaagatat tcacctgccg ccagaccatt
     1201 cagacagcgc gccgcacccg cgaaaaagcg aaaaacatcg acaatcgccg ggatttcatc
     1261 attgaacgca ctatgcagcg gtttgccaca attacgggac tccagttcgg caaaaacctg
     1321 accattttct tcgataacat cagccagttt cagcagacat tccgcacgca ctttcggcgt
     1381 ggtttgcccc cattcggcaa atgctgcatc tgccgcgcgc acagcagcat cgacctgctc
     1441 tgcggatgcc tcggcaatct ccagtaaaac gtcccccgtt gccggattat agacaggctg
     1501 tttttcccct tcgccgctaa ccagttctcc gttaatcagt aacttatgtt gcatagcata
     1561 ttcctgtatc agtggttatt gaccattgtc gccttcgcgg gttagccacc aggcccccag
     1621 gatcggcaag gttgttacca acataaccag cagtgccacc acgttagtta ccggtacatc
     1681 acgcggtcgc ccaagctgat tgagcaacca caacggtaac gttcgttcat gacctgccgt
     1741 aaaagtcgta acgatgattt catcgaacga caaggcaaac gccagcattc ctcctgccag
     1801 tagcgccgaa ctgagattcg gcaacactac gtagcggaag gtttgccagc cattggcccc
     1861 aagatccatt gacgcctcaa ccagactcca cgaggtgcgg cgaaaacggg cgatgacatt
     1921 gttaaacacc accactacgc aaaaagtcgc atgaccgacc acgatggtga aaaatcccgg
     1981 ctccagattg atggttttaa aagcggttaa tagcgccaga ccagtgacaa tgcccggcag
     2041 cgcaatgggc agcagcagta acagcgaaat ggcgtttttg ccgaaaaagt ctcgtcgcca
     2101 cagcgcggca gctgccagcg tccctaacac cagcgccatt aatgtcgcca gcgccgccac
     2161 tttaagtgac agtgtcacgg catcaagaat atcactacgc tgtgctgcca cgctaaacca
     2221 gcgcagcgtc aggccctgcg gtggaaaact aaacgccgca tcttcagtgt taaacgcata
     2281 agcggcgata atcaggatgg gaaaatgtag gaaaaccacg ccgccccagg ccgccagttt
     2341 gaggaaaaac ggtgcgcgtt cagagtgcat cgaacgctcc cagacgtttc acgaacgcca
     2401 ggtacagtgc gatgagaaca atcggcacca gggtgaatgc cgccgccatc ggcatattgc
     2461 caatcgcccc ctgctgggaa taaaccatat tgccgataaa atatcctgga ggaccaacca
     2521 gctgcgggac gataaaatcg cccagtgtga gtgagaaggt aaagatagag ccagcggcaa
     2581 tacccgggat tgccagcggc agcaccacat agcgaaaggt ttgtcgtgga cgtgcgccga
     2641 gatcagccga cgcctgcaac aatgacggcg gcaaacgctc aagcgccgcc tgaacgggca
     2701 ggatcatgaa cggcaaccag atatagagaa acaccagaaa gcgccccagc ccggaagttg
     2761 acagcgtatt tccgcccacc gcaggtaatg taaggaacgc agtcagcagt ggttccagcc
     2821 caagatgttg taaaaaccac tgagccacgc catcttttgc cagcaataac gtccaggcat
     2881 aggctttaac aatgtagctc gcccacatcg gcaacattac cgcaatataa aaaaaagctt
     2941 tcattttccc gctggtatag cgcgccatat accacgccat tggaaaagcc agaatggcgc
     3001 tggcgatagt gactgccaca gccatagtca gcgtgcggag aatgatgtcg taattcgccg
     3061 gattaaacag cgcacggata ttcgccagcg tcagttccgg cgttaccgac atggtgaaat
     3121 cgtcaaaagt ataaaatccc tgccataaca gtgtcagcag cgagccgaaa tagacaatgc
     3181 caaaccacat tagcgggcca agcagcagta aaaacagccc cagcctcgga ttacgccaga
     3241 agaaaccgga caccttaccc agacctggac gtgaaggtga ttgcaataca ttcatcgcca
     3301 ttcacctctc ctcaaccagc ggcaccatca catcacgcga ccaggaaacc atcacctgtt
     3361 gtccgggcgt gagcgtggca ggcagttctt cgcctgtcat attggcctga ctcacaagca
     3421 gtttttcacc gccgttcaat ttcagttcaa aacgagttgc cgcgccctga tattgcaccg
     3481 cctggatcgt gccattggcc tgcagttcac caggggtgtt gaggcggata tgttccggtc
     3541 gcagggcgaa gcttcccgtc atgccacaaa gtttctctgc catcagtcca tcaaaaacgt
     3601 tggatgtccc gacgaatccg gcaacaaacg gcgtgcgcgg gcgcatatag agatcgcgcg
     3661 gggaatcgac ctgctcaatg cgtccattat tgaaaaccgc cacacgatcg gacatcgata
     3721 acgcttcgcc ctgatcgtgg gtaacgaaga taaaagtgat accgagagac tgttgcagtt
     3781 ttttcagttc cagctgcatc tgctcacgca atttgagatc cagtgcgccg agcggttcat
     3841 ccaacagcaa tacgcgcggt tcattcacca atgctctggc gatagcaacc cgctggcgct
     3901 gaccaccaga aagttgtgac ggtttacgtt gatgtacaaa ccccaacgcc actttctcca
     3961 gcgcctcttg cgccattgcg tgccgctgct ttttattcac gcctttgacc atcagcccat
     4021 aggcgacatt gtcaagaatc gacatatgcg gaaatagcgc gtagtcctga aagacagtat
     4081 tcacgtcccg ctcccacggt ggcagattgc tggcgggttt accaaagata gagatagccc
     4141 cgccggaaag ctgttcgaag ccagcaatca ggcgcaggca ggtggttttg ccggagccgg
     4201 acggccccag catagagaag aactcaccat cttttatcgc aatactgacg ccatctactg
     4261 cgcgcacgtc accgtacaac cgcgagacgt tgtcaaactc cactgcgtac gtcataaagc
     4321 accccagcga agttagcgac cgcccataat ggcaatgtaa tcctgcgtcc agcgactgta
     4381 gggaacaaac ttgccccctt ctgctatagg cgttttccag aaggcgattt tgtcgaaata
     4441 gttaaaaccg ttggtttcac aacctttttc gcctaataac ggactggctt tacacccttc
     4501 cggcactacc ggtaacgagc caaaccaggc cgccacatcg ccctgcactt ttggcgttaa
     4561 tgaccagttc atccatttgt aggcgcaaac cggatgtttc gcttcgctat gcagcatggt
     4621 ggtatcagcc caaccggtaa caccctcctt cgggaaaacg gtagcaacag gctggccttc
     4681 ggctttcagg gcgttggcct gatagggcca ggcactggaa gcaaccacac cctcgttttt
     4741 gaaatcgctc atttgcacgg tagtgtcatg ccagtagcga tggatcaaac tgtgttgagc
     4801 gcgcagcact ttcagcaccg cctggtactg ttcttcggtt agttgatacg gatcgctgat
     4861 gcccaactgc ggctgagtgg ctttaacgaa caacgcagcg tccgcaatgt agatagggcc
     4921 atcataagcc tgaacgcggc ctttattgct cttgccgtcc ggcagatttt gctcaacaaa
     4981 aaccacttgc cagctatccg gcggcgtcgg gaaggtttta gtgttgtaca tcagcaggtt
     5041 cggcccccat tggtaaggtg tgccgtaaac tttgccgcca acattaaacc agtcgccttt
     5101 aaccacgcgc ggatcgagcg ttttccagtt gggaatcaag gcggtattaa tcggctgcac
     5161 gcgtttgccc ataatcaaac gcagcgaggc atcgccggat gccgtaacca gatcgtaacc
     5221 ccctttggtc atcagactga ccatttcatc ggaagtcgcg gcggttttca cattcaccgc
     5281 gcagcctgtc tctttttcaa attgcgttac ccagtcgtat tgtttatcag tttgtccgcg
     5341 ttcgatgtat cccggccagg cgataatatc cagtcgccct tccggtttat ctaaattggt
     5401 aggtggttcg gcggcgtgag cggtcattat tgtcatggtg agcgcacaca ggctgctgcg
     5461 ggcaaatgtc ttgctcataa ggtcttactc ctgtcgtaat taattgttca gccgccgtgc
     5521 cgtaaaaata tctccttgtt taaccataga cggcggcttg caggcacacc ttcgcccctg
     5581 agaaaattta atcaggttgt gagctaccgc gcattatgcc gtctgtaagg ttatcttctg
     5641 gagtatagac aaggggttag gaaatatgac tgctattcga aattcatatt acccaatcta
     5701 agaggaatta aattaaacgc tattacagcc gttcttgaat aagtttgcct aattgtttta
     5761 ccgcctgttc ttcacgttct ccccactgcc acgcggtatt aaaacgaaaa aaacgtgacc
     5821 agttttcacc ggtagaaaac attttacccg gcgcaatact gatatgatgc gtcagtgccg
     5881 ccaggcttaa ttcgccggca tctaacggct cggggagctc cagccagaga aaataaccgc
     5941 tgtcactatg atgaatttta acttccgcag gcagataacg cagcagtgcc tgccaggcac
     6001 gttgtttacg ttccgcaagc tggcgacgca ggcgacgaag atgggcgtcg tatcggcgcg
     6061 tggaaaggta atccaccagc gcaagctgca tcggtgagct ggtggaaagc gtactcatca
     6121 actgcaagcg ttgaatttta cgtgcatgtt ttccggcggc gacccaacca atacgaaaac
     6181 caggcaccag acatttcgaa aacgaagagc aatgcaaaac gccatcgtgg cgatcccacg
     6241 ctttcgcagg cagcggtttt tcccgtccaa aataaagttc gctgtaaacg tcatcttcaa
     6301 tcagcgttac gttgtactga ttgagtaacg ccaccagttg tgcttttttt gcggcgttaa
     6361 ggtaaatccg agtggattct ggctattagt catcagccag cacgctttca ccggatactc
     6421 ctgcaacgcc agttccaacg cctgaagatc tatcccttct ttaacatccg tcgccaccga
     6481 taacgctttc agccgtagcc gttccagcgc ctgcaacgcg ccatagaaac aaggattctc
     6541 tactatcacc caatcgcccg gttcagttac cgcttgcaaa ctgaggttta atgcctctaa
     6601 cgccccggca gtaatgacaa tttcatcagg agaaatggtg atgccctgta aggcatagcg
     6661 acgagcaata gcctgacgca gttctgcgtt tcctggcggt aagttttcaa tcacgctcat
     6721 cgccgtggcg gttttgctta cctgcgccag cgagcggttt agttgttgga gggggaaaag
     6781 tcgcgggtcg ggaaaggccg aggcaaacgg aacgaccgac ggatcgcgac tggcctgcaa
     6841 catatcaaaa atataagtgt tgatatcgac tgcttcatct cgagtgactg gaatgactgg
     6901 cgctttcggc atttttattg cctgtggcgc aacgtaataa cccgactgcg gtcgtgcgat
     6961 aatatatccc tgactttcga gcaactgata ggcatggctg acagtcataa agctcatgcc
     7021 tgaaagcgcc acctggtcac gcaacgaagg caaacgatcg ccgggttgcc agatacccga
     7081 cgcaatctgc tcgcgtaatt gttctgcaag ctgctggtat tttttcatta tcgaatcgta
     7141 attatgtgca gatgattcgg cagtctatat cagttgttaa aaatgacaac ttttcgttaa
     7201 ctgtaattaa accatcacca gcgataactc tttgccaagc gcctttgccg caagctggac
     7261 ggcgtcgatt tttgtcgcat ggtgcaagtt aaataggcga gtaatctcct gtttaggttt
     7321 gccaattcgc ctggctaact cttgctgagt gatttctgac tgtaaaaaag catttaacag
     7381 caatacttta gaggcgacgc tcaaaggtac ttcaataaag tgatcatgac tatttaatgg
     7441 cgaaggtaac gggataagct cgttatcttc aaaataaaaa tcaaatgcgg tcagtaaagc
     7501 atcttttgcc gcttccatcg cttcagcgac agtttcgccc tgcgtcaacg cttcagggat
     7561 atccacaaaa gaaaccatat aaccgccttc cggcgcgggt gtaagagtga cgggataacg
     7621 cataatttcg actgtccctc gcatgtcatt aaccttttta attggcgatt aactcaaacc
     7681 gagttgtttc aggattgctt tacgcaatgg ttctttaatc tcatcgcagg ggtgacgcgg
     7741 catgacactg cgcctcccat gaaacctgag tttcaaatgg ttgctgccat tcgctacatc
     7801 gacgccctga gattcgagcc aacgtctgaa ctcgctttgt ttcacatcgc ctccctgccg
     7861 ctgagtatat caacaaagta aacattagtg tttacctccg tgtgtaattt gctcgaaaat
     7921 agcactaaat cgccgcaacg gcagaatgcc atgcaaaatc aggaagaaag actccagaat
     7981 ttctttgcgc taatttgcaa aactgaataa taacctcaag aaacgatcgg catcatagcg
     8041 gcaaaaggta agaaaaaggt ggcaggatgt ttacgaaggc gttatcggtt gtcttattaa
     8101 cgtgtgcgct gttctcagga caactcatgg cagggcacaa aggacatgaa tttgtgtggg
     8161 taaagaatgt ggatcatcag ctgcgtcatg aagcggacag cgatgaattg cgtgctgtgg
     8221 cggaagagtc ggcggaaggt ttgcgcgagc atttttactg gcaaaaatcg cgcaaaccag
     8281 aagcgggaca acgttgagtc atttgttgcc ggatgcggcg agacgcctta cccggcaggc
     8341 aatatcatgc agattcaatg agttgtgagt aaccagaaaa ctggcgtttt acccttttcg
     8401 cttaggcaat gttttcagca gctcatccgg acttacggat gccactacgc ttcccggtaa
     8461 cggcattttc agtatgtgat ttttgatttt gccaatgacg tgcatctcgc atgggcggca
     8521 atcaaacttt agcgttaata cttcatcgcc gttaaccagt tgcattggcg tcgccttcca
     8581 gcttttgata ttgcctttcg cctgtttcgg gcacagatta aaggcaaagc gcagacaatg
     8641 cttggtgatc atcaccggga cttcgccctt ctcttcatgt gcttcatacg ccgcgtcaat
     8701 cagctgcaca ccatagcgat gataaaattc acgcgctttc tggttgtata cgttcgcgaa
     8761 gaaactcaga tgcgtttgcg gataaaccgg cgcaggatca gcaaccggtt tacggctgcc
     8821 gcgctggtaa ctggcaagac gcgcagcatc cagcatgtca gcagcttcac ggcggaactg
     8881 gtttaacaga ctgtttggta caaacagcgc ccccggcaaa ttaatttgca catcgcgggc
     8941 gtaatagagg gtttgcccca gttttgccag accatccttc agattgttca ttgctttttc
     9001 ggcgttattg gcttcgtcga actgcccgtc cagcgtatgc gtgatgctga caccctcttc
     9061 actggtgagg gtcagaatca gttgttcctg ccagccgccc agttcaatgt ctaccgccac
     9121 ccgacgttcg ctggaggttt ttgtcagtgc ctgctgccag ttatgatcaa gattacggtt
     9181 tagtgggtga tgtggacgaa ttttgtgcaa atctgctggc atttcattgg gccagacgcg
     9241 gtactgattt tctccggttt tctcgaccgt attggcacga aaaccgacga cttcacgttt
     9301 aatcatcacg ttcaggccat caccatttgc cagtggctcg gtgacggcaa catcgagatg
     9361 atctttcgcc actttcaata cttcgcctac cggcaggccg ataaatttcg gcgaatcgaa
     9421 cgcgccaata tcgcctttac gggcattcac aaaataatct gtgctaccac ggtggaaagt
     9481 cttttccgtc gatggaacaa agaaatgttc agtacgacct gatgaagcgc gcgccagatc
     9541 gccacgttct tcaataatgg catcaagcat ctggcgataa tgggcggtga tattcttcac
     9601 gtagctcata tctttgtaac gcccttcaat cttgaaggag cgtacgccag catcaatcag
     9661 cgcgccgagg ttggcagtct gatcgttatc tttcatcgac agcagatgtt tttcatagga
     9721 aaccacccgc ccctgatcgt ctttcaatgt gtatggcaaa cggcacgcct gcgagcaatc
     9781 accacggttg gcgctacgcc ctgtttgcgc atgagaaatg tagcactgac ccgaataggc
     9841 cacgcacagt gccccatgaa taaagaattc aatggtcgcg tccgtggcct ggtgaatcgc
     9901 gcggatctgc tcaagattca gctctcgcgc cagcacaatc tgcgtgaagc caacatcaga
     9961 gaggaacttc gctttttcta ctgtacgaat gtcgcactgc gtactggcgt gcagttcaat
    10021 cggcggaata tcaagttcca gaatccccat atcctgaaca atcagcgcat cgacaccggt
    10081 ctggtagagg tcggtaatca gccgttgcgc gggttccagc tcatcatcat gcaaaatggt
    10141 gttaagcgtg acgaaaattt ttgcaccata acgatgggca aacggcacca gctcggcaat
    10201 atctttcagg ctattactgg cattatgacg ggcaccaaaa ccagggccgc cgatataaac
    10261 agcatcggca ccgtgcaaaa tagcttcgcg ggcaatggcg gcatcgcgtg ccgggcttaa
    10321 cagttcaagt cgatgagaag atacggtcac attcttatcc agtagctgaa aaatggcggc
    10381 tattttagcc acaagcgcga cgttgcgaaa tagttttccc gcttaactgc gcgggtaatg
    10441 gatgagggaa tgaaaatgta cggtttgctc cccaccatta cgatagatat gcgtgacgtc
    10501 tgcagcaaat cgaacgcctt ccccacaatt aagggtctgc cattccccat caacgcacag
    10561 atcaagttgt ccatcaatga cgacgacatg ttcgattacc cccttctcat ggggcgtcga
    10621 ttcgcttatt gcaccagatg ccatctgaat cgagaagtgt tcgaagcaga gttgtggatc
    10681 ataaggaaac agcgaagtga tcaccatcgc ctgctgttgt ggatcataaa ctgaaggcgt
    10741 agcggactgc ggcggagaaa taaatgttga aaatggtacg ttcaacccgg tggcaatttt
    10801 ccataatgtc gcgaccgtcg gactggactc attacgctca atctgcccta acattgcttt
    10861 agaaacgccc gtcgcttctg ccagtcgcga aagactccag ccgcgctgct ggcgtagttg
    10921 tttaagtgtg gtggataaaa agcgagcgag attttccatg cgactcctcc ggcaaaacgg
    10981 aagtttatca cttgtgcgtt ataacggaca aatgctacgg tgcctgtacg ctataacgca
    11041 cgaggtgact atgcgtctgt tttctattcc tccacccacg ctactggcgg ggtttctggc
    11101 ggtattaatt ggctacgcca gttcagcggc aataatctgg caagcagcga ttgtcgccgg
    11161 agccaccact gcacaaatct ctggctggat gacggcgctg gggctggcaa tgggcgtcag
    11221 tacgctgact ctgacattat ggtatcgcgt acctgttctc accgcatggt caacgcctgg
    11281 cgcggctttg ttggtcaccg gattgcaggg actaacactt aacgaagcca tcggcgtttt
    11341 tattgtcacc aacgcgttaa tagtcctctg cggcataacg ggactctttg ctcgtctgat
    11401 gcgcattatt ccgcactcgc ttgcggcggc aatgcttgcc gggattttat tacgctttgg
    11461 tttacaggcg tttgccagtc tggacggtca atttacgttg tgtggaagta tgttgctggt
    11521 atggctggca accaaggccg ttgcgccgcg ctatgcggta attgccgcga tgattattgg
    11581 gatcgtgatc gttatcgcgc aaggtgacgt tgtcacaact gatgttgtct ttaaacccgt
    11641 tctccccact tatattaccc ctgatttttc gtttgctcac agcctgagcg ttgcactccc
    11701 cctttttctg gtgacgatgg catcgcaaaa cgcaccgggt atcgcagcaa tgaaagcagc
    11761 cgggtattcg gctcctgttt cgccattaat tgtatttact ggattgctgg cactggtttt
    11821 ttcccctttc ggcgtttatt ccgtcggtat tgcggcaatc accgcggcta tttgccaaag
    11881 cccggaagcg catccggata aagatcaacg ttggctggca gctgccgttg caggcatttt
    11941 ttatttgatc gcaggtctgt ttggtagtgc tattaccggg atgatggctg ccctgcccgt
    12001 aagttggatc cagatgctgg caggtctggc gctgttaagt accattagcg gcagtttgta
    12061 tcaggcgctg cataatgagc gtgagcgcga cgcggcggtg gtggcatttc tggtaacggc
    12121 aagtggattg acgctggtcg ggattggttc tgcgttctgg ggattaattg ccggaggcgt
    12181 ttgttacgtg gtgttgaatt taatcgctga cagaaaccga tattgacatc ctccacgccc
    12241 tgaaggacga ggatccctgc tacgttcagg ctgtcgcctg aatcatttcg gtgggttcct
    12301 gcttcaacgg gcggcctgac tgcaccatct ctccacaggc aagaacggcg tgccccgccg
    12361 ctaaaatgtt acgagcgcca tttacatcgg cgttcgctgt atatccacat acctggcatc
    12421 tgaatttact ttgtgacagg cg
//
LOCUS       DAZGJW010000132        12301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZGJW010000132 DAZGJW010000000
VERSION     DAZGJW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.132"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..729
                     /gene="ybjI"
                     /locus_tag="ACHKIK_003861"
     CDS             <1..729
                     /gene="ybjI"
                     /locus_tag="ACHKIK_003861"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO9084941.1"
                     /translation="YQQMKAQGIRFVVASGNQYYQLISFFPEIANEIAFVAENGGWVV
                     SEGKDVFNGELSKDAFATVVEHLLTRPEVEIIACGKNSAYTLKKYDDAMKTVAEMYYH
                     RLEYVDNFDNLEDIFFKFGLNLSDELIPQVQKALHEAIGDIMVPVHTGNGSIDLIIPG
                     VHKANGLRQLQKLWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVVAAAKYRA
                     GSNNREGVLDVIDKVLKHEAPFDQ"
     gene            815..1099
                     /gene="ybjH"
                     /locus_tag="ACHKIK_003862"
     CDS             815..1099
                     /gene="ybjH"
                     /locus_tag="ACHKIK_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO9084942.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(1140..2372)
                     /gene="mdfA"
                     /locus_tag="ACHKIK_003863"
     CDS             complement(1140..2372)
                     /gene="mdfA"
                     /locus_tag="ACHKIK_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO9084943.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            2657..3253
                     /gene="ybjG"
                     /locus_tag="ACHKIK_003864"
     CDS             2657..3253
                     /gene="ybjG"
                     /locus_tag="ACHKIK_003864"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO9084944.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            3311..4069
                     /gene="deoR"
                     /locus_tag="ACHKIK_003865"
     CDS             3311..4069
                     /gene="deoR"
                     /locus_tag="ACHKIK_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO9084945.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(4116..5318)
                     /gene="dacC"
                     /locus_tag="ACHKIK_003866"
     CDS             complement(4116..5318)
                     /gene="dacC"
                     /locus_tag="ACHKIK_003866"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO9084946.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            5565..6191
                     /gene="gstB"
                     /locus_tag="ACHKIK_003867"
     CDS             5565..6191
                     /gene="gstB"
                     /locus_tag="ACHKIK_003867"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO9084947.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(6188..7303)
                     /gene="yliI"
                     /locus_tag="ACHKIK_003868"
     CDS             complement(6188..7303)
                     /gene="yliI"
                     /locus_tag="ACHKIK_003868"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO9084948.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(7414..7797)
                     /gene="bssR"
                     /locus_tag="ACHKIK_003869"
     CDS             complement(7414..7797)
                     /gene="bssR"
                     /locus_tag="ACHKIK_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO9084949.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            8010..9335
                     /gene="rimO"
                     /locus_tag="ACHKIK_003870"
     CDS             8010..9335
                     /gene="rimO"
                     /locus_tag="ACHKIK_003870"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO9084950.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(9382..10710)
                     /locus_tag="ACHKIK_003871"
     CDS             complement(9382..10710)
                     /locus_tag="ACHKIK_003871"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO9084951.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYYAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(10718..>12301)
                     /locus_tag="ACHKIK_003872"
     CDS             complement(10718..>12301)
                     /locus_tag="ACHKIK_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFO9084952.1"
                     /translation="IAKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLL
                     VVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMK
                     DAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDE
                     RNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYR
                     NLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIA
                     RNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGF
                     INELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGL
                     KANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSI
                     KVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYL
                     YQKPMPFSAWDKSGKLVKE"
BASE COUNT         3212 a         3118 c         2864 g         3107 t
ORIGIN      
        1 tatcagcaaa tgaaagcaca aggcattcgc tttgtggtcg ccagcgggaa tcaatattat
       61 cagttgatct ctttcttccc tgaaattgcg aatgaaattg cctttgtggc tgaaaacggc
      121 ggctgggtag tgagtgaagg caaagatgtt tttaatggcg agctgtcgaa ggatgcgttt
      181 gctactgtcg tggaacattt gctgacgcgc ccggaagtgg aaattattgc ctgcggaaaa
      241 aatagcgcct atacactcaa aaaatatgac gatgccatga aaacggtggc ggaaatgtat
      301 tatcaccgtc tggaatacgt cgataacttt gacaacttag aagatatttt ctttaagttt
      361 ggcctgaatc tttccgatga actgattcca caagtacaaa aagcattaca tgaggccatc
      421 ggcgatatta tggtgccagt ccacaccggc aacggcagca tcgatctgat tatccccggc
      481 gtacataaag ccaatggcct tcgccaactg cagaaattat ggggaataga cgacagcgaa
      541 gtggtggtct ttggcgatgg cggtaacgat attgagatgc tgcgtcaggc aggctttagt
      601 tttgcaatgg aaaatgccgg cagcgcggtc gtcgcagcgg caaaataccg cgcaggctcc
      661 aataaccgtg aaggcgtact ggatgtgatc gataaagttc ttaaacacga agcgccattt
      721 gaccaataaa aacggcagca cgcccggcaa cacaatgcct gaatgaaggc tgcataattc
      781 gcggaaatta acccgctgca ttatttcagg cgaaatgatt atgaaaaatt gtctactgtt
      841 gggcgcgctt ttaatgggct ttactggtgt ggcgatggcg caaagtgtca ccgtcgatgt
      901 gccgagtgga tacaaagtgg ttgtggttcc tgattcagtc agcgttccac aagcggtcag
      961 cgtcgcgact gtgccgcaaa ccgtctatgt ggctcctgcg ccagctccgg cttaccgccc
     1021 gcatccttat gttcgccatc ttgccagcgt aggtgaaggg atggttatcg aacaccagat
     1081 tgacgaccat caccattaat tcgagaatgc ctgatcgcac aaagcaatca ggcatttttt
     1141 tacccttcgt gagaatttcc catctgttta tcttttaaaa agataaccat cagcgacagc
     1201 cacaaaattc cgttgacaag gttgaagaga ttaaacagtc cgttgccccc gttcagccag
     1261 gcatgtttgc tgatttcaat accgacggta aagatcagca tttgcagcat tcccatcgcc
     1321 gcagaaaccg tacctttact catatcgctg gcaaacaggg ttaatcgcac cagtcccgca
     1381 ttcgccagac caatgccgaa agcataaata cttaacccgg cggtcatcca taaatacgcg
     1441 tgcgatgaga taaccgttgc cgcagcagcg accaacagac caatcataat cggccagccg
     1501 cccataatta tcagcgaacg tacggtgcgg cgcgaggtca aacgcgctaa caacaagtta
     1561 cccgcaatta acgccccgaa aataggcact tgcagcaagc catattcata gctgctcaac
     1621 tgctcgccgg taatgatgat aatcggcgac tgggcgatcc acgccagcaa tggcaggcta
     1681 acgaatccca gcgccagcgc ccccgccaca aagcggccgt tcttcagcac cagcttatag
     1741 tcacgaccga gttctttcag tgacagtttc tcgcctatac gcgtggcggt ttcaggcatg
     1801 gcttgttgca gaccgaaaaa ggagatcgct gccaatgcgg caaacaagac aaacatccct
     1861 tcccagggca gcacatggat ccacgccgcg cccaccagcg gaccaagtag cggagcaatc
     1921 agcgccacgt tcgccatcag cgcggtgatc ttgatacaaa ccgcctcttc gaaggattcc
     1981 tgaattgcgg cgtatcccac agcgccaatg aaacagaggc ttatgccctg caagaagcgc
     2041 aacagagtga attgttcaat gttttgcgcc agtaatattg ccagacaggt gatgataaac
     2101 cacaccaccc ccgccagcat caccggacgg cgaccaatac gatccgacag cggccccagg
     2161 agccattgta aaaacatccc gcccgccaga tatgcggtca ttgaagtagg aacccaatca
     2221 atgcccgcct gatattgttc caccacggcc aacataccgg gttgaatcat atcgttgccg
     2281 atataggttg aaaattcgta aagcaccaga cagagaggga aaagtaacgc ctgacgtcca
     2341 agcctggcac cggaagctaa tttattttgc atgcaatttc ttcgccaata ataatcgcgc
     2401 agagtttaat aaaagcacag ctaacgagaa agcgaatttt gtagctgaaa ccacggttaa
     2461 gcacattctt acattattgc gagtatagct acgctttctt taagttttat ttaacctatg
     2521 cccgttacaa ttacccaccg taaacaggcc gcttgaggga aataagacga tgccgcttta
     2581 cccagtttaa cctgcacttt attctcaacg acttgcctgt attggctccc ttttaatcac
     2641 tttgcgtcgg gaagttatgc tggaaaattt gaatctctct ctattctctc ttattaacgc
     2701 gacgccagac tcggctccgt ggatgatctc gttggcgatt tttattgcta aagatttgat
     2761 taccgtggta ccgctgctgg ccgcggtgct ttggttgtgg gggcttacag cacaacggca
     2821 actggtgata aaaatcgcta tcgcgctggc ggtcagcctg tttgtgtcct ggacgatggg
     2881 acatcttttt ccgcacgacc gaccctttgt cgaaaatatc ggctataact tcctgcatca
     2941 tgcggcggat gactcattcc caagcgacca cggtacggtg attttcacct ttgcactggc
     3001 atttttatgc tggcatcgcc tgtggtccgg ctcactttta atggtgctgg ccgtcgtcat
     3061 tgcctggtcg cgcgtttatc ttggcgtgca ctggccgctg gatatgctcg gtggattgct
     3121 ggctggtatg attggctgcc ttagcgctca gattatctgg caagcgatgg ggcataaact
     3181 ctatcaacgt ctgcaatcgt ggtatcgctt ctgttttgca ttaccgatcc gcaaaggctg
     3241 ggtgcgtgac tgatctctgc taaaaagtgt agtattgagc ggctcgcttc aataactatt
     3301 cagagggatt atggaaacac gtcgcgaaga gcgcatcggg cagctgctgc aagaattaaa
     3361 acgcagcgat aagttacatc ttaaagacgc cgccgccctg cttggggttt cggagatgac
     3421 gattcgtcgc gatctgaaca accacagtgc gcccgtcgtt ttgctcggcg gctatattgt
     3481 tctggaaccg cgcagtgcca gccattacct gttaagcgat caaaaatccc gcctggtgga
     3541 agaaaaacgc cgggcggcaa aaatggctgc gacactggta gaacccgatc agactctctt
     3601 ttttgactgt ggcaccacca cgccgtggat tattgaagcg attgataatg aaatcccttt
     3661 taccgccgtt tgttattcgc taaatacctt tctggcgctg aaagagaaac cccattgccg
     3721 cgcatttctg tgcggtggtg aatttcacgc cagcaacgcc attttcaaac ccatcgattt
     3781 tcagcaaacg ttgaataatt tttgcccgga tatcgctttt tattccgcgg cgggcgtgca
     3841 tgtcagtaaa ggcgctacct gttttaatct tgaagagttg ccggtaaaac actgggccat
     3901 gtcgatggcg caaaagcatg tgctggttgt cgaccacagt aaatttggca aggtgcgtcc
     3961 ggcgcgcatg ggtgacctga aacgctttga tattgtggtg agtgattgtt gcccggaaga
     4021 tgagtatgtg aagtacgcgc agacgcaccg tattaagttg atgtattaat gacgtataac
     4081 cggatgacgt ttcgcgccat ccggttatca gaagattaag agaaccagct gccgaaccac
     4141 tgatggaatt tcatcatcac gaaatcccac atccgaccaa agaatccgcc ctcttccaca
     4201 ttttccatca cgatcagcgg acgctgctca atggatttac cgttaagctg gaaatcaatg
     4261 gtcccgacaa cctgaccttt tttcagcggt gcggtaagct gcggttccgt taacgtgtag
     4321 ctcgccttca ggtttttcag ctgcccacgc ggtatggtca ctgagcccgc ttcgcctgcc
     4381 ccaagattca cttcgctctt atcaccaaac cagacgcgct gagtcacaaa ggtggcatca
     4441 ggtttaattg gcgtcacggt ttcaaagaag cggaaacccc aggtcaataa tttctcagac
     4501 tcattaaaac ggatacggtc agttttcgcc cccagcacta cggagattaa acgcatatcg
     4561 ccctgggtag ccgaagcaac cagattatat cccgcgcctg ccgtggttcc tgtcttcatg
     4621 ccatcaacat tcagattgct gctccacagc agacggttac ggttaggctg acgaattttg
     4681 ttgaaggtga actctttctc tttatgaatg gcgtactctt ccggcacatc gtggatcaat
     4741 gctttaccca gcaatgccat atcgcgcgcg gtactgaact gccccggcgc atccagaccg
     4801 tgcaccgtct ggaaggtagt gttggtcaga cccagttttt tggcataacc attcatcaaa
     4861 ccaatgaatg actcctggct cccggcaacg taatcggcca gcgcaataca ggcgtcatta
     4921 ccggactgga taatcacacc tttgttcaag tctgccaccg aaacctgatc gcccggtttg
     4981 aggaacatta ccgatgaacc acgcagtgcc ggatttcccg tcgcccaggc atctttaccg
     5041 accgtcacca tatcggtgag tttaatctta tcggccttaa gcgcctgccc aaccacatag
     5101 ctggtcatga ttttagtcag gctcgcggga tccagtttct catccgcgtt gccttctgcc
     5161 agcactttac cgctggcgta atccattaaa atccatgcac gcgcatccac gctcggcgct
     5221 tcaacggttt gttccgccgc gaatgccgtt ggggcaaaaa ggaataaaaa tgcagaaccc
     5281 gctgcaagac cacgaaggag agaggagtat tgcgtcataa atgccacccg agtatccatt
     5341 caaaaaaaat acgttatatc gccgtatgat aagttcggtg attaataacg cacacacaaa
     5401 cttaaagaaa ccgtaagttg gtaaagtttt taaagtttat gcaatatcct gccgcttcgc
     5461 tgcttctggt gtcatttttt tgccagctgt tgcttaaaag ttaactttac ttcaccatcg
     5521 gagtcctgtt cagcctgtgg ctgcctgtcg gaaaaggggt gagtatgatt acgctgtggg
     5581 gtcggaataa ttcaaccaac gtaaaaaaag tattgctgac gctcgaagaa ctggaactac
     5641 cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct gattttctgg
     5701 cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat ctcattcttt
     5761 gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa cgtctgtgga
     5821 tcgactcacc ggcacgtcgt gcggaagcgg aaaaatggat ggactgggca aaccagacgc
     5881 tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg gaagagcgcg
     5941 atcaggccgc cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc ctgctcgatg
     6001 cggaactggc aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc gatatcgcta
     6061 tcgcaccgtt catctacaat ttgtttaacg ttggcctgac ctggacaccg cgtccaaatc
     6121 tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg gtgatgattc
     6181 ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg tcggtgagaa
     6241 cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct ctgtccgtta
     6301 aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg acatctttat
     6361 ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttatcg ctgttataga
     6421 aggccatgcc gctcacagcg ggcgaatctt tccagtaaaa aacaggttgc tcggtcccgg
     6481 cgacgatctc ccctttcgct tccggtatct taaagcctga atagttgatt ccccaggttg
     6541 ccagcggcca gccgtagttt ttgccttttt gcgggatatt aatttcatca ccaccgcgcg
     6601 ggccatgttc attcagccac agtgcattac tccacggatt cattgccatt ccttgcggat
     6661 tacgaatgcc ataagaccag atctcggcgc gcacaccgga ttcctttata aaaggattat
     6721 catccgggat ttcgccctgg tcggtcagac gcaccagttt gccctgtaat ttatccagat
     6781 cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat aaaaagataa cctttaccgt
     6841 cgaataccag ccgcccgcca aaatggttgc cggtagacag ttttggcatc tggcggaaga
     6901 cggtgcggaa gtcggtcact tttgagagat catcacttaa gcggccataa cccacagcag
     6961 ttccggcttt gccatcatcg ccaacttcgg aataacttaa ccagatgcgg cgagactgag
     7021 caaaatcagg cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc caaacgtccg
     7081 gaactccgga aagcggcgca gataatcctt ttcctgcttg ccagtgacgc aactcgccgc
     7141 cgcgcagagt gattaacata ccgtgattat cgggtaaaaa ggccagtgcc cagggatggt
     7201 cgagtttgtc ttgcagtact tcgacattta ccgttgcagg agccgcccag agagcggaag
     7261 aaagacaaat aaggggcaca aggaaaaagg attgtcgatg cataacgcct ccggattgac
     7321 tctggcttaa agcgtagtca gtggaggaga taacaaattc atttttacaa aaacttaaac
     7381 atgaaggggg agacgctttc tcccccttag ttttcaggcc ttctcaagca tggcgtgctt
     7441 ctgcaggctc tggatactca gcgttaagct catcagacaa ttttcaagct tatcggcgtt
     7501 gacggtaata acagtcgggc aatcatggtg cccactcatc aaacatactg cggctgtcgc
     7561 taatgcttct tcagcatgat gaagagcact ccactcttcc tgatccagat gaagattcaa
     7621 ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc gtagatgatt
     7681 gctttcgctg acggacatgt atccttttgc cttcctcagt tgcacgtatg cagcgaggtc
     7741 aacgcgtgcg tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa cgaacatact
     7801 tcgatcctcc tctttccagc cttgttgcaa ttaaatagta tggctatttt ttgagcaaaa
     7861 tgctgtccag gatcaattct tcacgccgaa ttaacaaaat ttacatttcc ttcgcctccg
     7921 ccagcgaatg agattgcgat acaaatttta tccagaactc tgtagaatcc ctgccctgac
     7981 ctttcctgta actgaatatt ttttaagcta tgagcaaagt aactccccag ccgaaaatcg
     8041 gctttgtttc ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt ctcaccgaac
     8101 tacgcactga aggttatgac gtggtaccga gctatgacga tgcggacatg gtgatcgtca
     8161 acacctgcgg ctttattgac agcgcggtac aagaatcact ggaagccatt ggtgaagcgt
     8221 tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa gatcagatcc
     8281 gcgaagtcca cccgaaagtg ctggaaatca ccgggcctca tagctatgag caggttctgg
     8341 agcacgttca tcactacgtg ccaaaaccga aacacaaccc attcctgagc ctggtgccag
     8401 aacaaggtgt gaaactgacg ccgcgtcatt atgcctatct gaaaatttcg gaaggctgta
     8461 atcaccgctg caccttctgc attattccgt ctatgcgcgg cgacctggtg agccgtccga
     8521 ttggcgaagt gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa gagattctgg
     8581 tgatctcgca ggatacttct gcctatggcg ttgatgttaa acatcgtact ggcttccaca
     8641 acggcgagcc ggtaaaaacc agcatggtca gcctgtgcga acagttatcg aaactgggga
     8701 tctggacacg tctgcactac gtttaccctt atccgcatgt ggacgacgtc atcccactga
     8761 tggcagaagg caaaatcctg ccgtatctgg acattccgtt gcagcacgcc agcccgcgca
     8821 ttctcaaact gatgaagcgt ccgggttctg tagatcgcca actggcgcgc atcaaacagt
     8881 ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt tattgtcggc ttccctggcg
     8941 agacagaaga agatttccag atgctactcg acttcctgaa agaagcgcgt ctggatcgcg
     9001 ttggctgctt taaatacagc ccggttgaag gtgcagacgc caatgccctg cctgaccagg
     9061 ttccggaaga agttaaagaa gaacgctgga accgtttcat gcagctgcag cagcagattt
     9121 ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct ggtgattatc gacgaagtgg
     9181 acgaagaagg cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac ggcgcggttt
     9241 atctcaatgg tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa gtcgagcacg
     9301 ccgatgagta cgatttgtgg ggtagccggg tttaattaat tcaggccggg caaaatcatt
     9361 tgtccggcct gcgaataatg attactgcca gctaatctga gccctgccgt ttttcttact
     9421 cttatacagc cgctcatccg ccagcatttt caagcgttcg acggtggtac attcatttga
     9481 atgcgctaca ccgatactgg tacttaaatt aatgcttccc acctggaaat gaacgatttc
     9541 ttttagaatc gatagcaact cttccagcgt tccacccgta taaatcaccg caaattcgtc
     9601 accgccgaag cggtagattt tacctttatc taccgggatc tgtgatttaa ggattttcac
     9661 cactttcatc aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa tgtttttgaa
     9721 tttatcgacg tcaaacaggc acaaataacc gcctttttcc ggcagcgctt ttaaatcttc
     9781 gtcaaaggat tttctgccca ttgctttggt caggtcgtcc aggaacgccg agttatacat
     9841 actgtagagt tttttgacat cggcagccga aaagaaaagc ccgccatcca gtacgcgttc
     9901 aaaatggtta ttaatgatgg cttctttctc cacaatacca cgattaatca ctctgcgtac
     9961 cagatagagg caactggtac ccgatatcac tgccaccaat gcggcaaaga aaataatcaa
    10021 tgtacagacc cgcaaataaa catctttttt atacgcaaat acgtacaatc cggtcggcaa
    10081 ataggcgaag tgctccatcg cttccgaaac ggtgaagatg gcgtttttag cataatattc
    10141 atcaccggtc accggtttac cgttcttaaa tagagagaac atcaggttct cgctgtccat
    10201 cgccagcgat tttgacagta aagaaaaacg ctccagacca agcaaaaata tcagactacg
    10261 attgtgagtt accggataga ggtaatagac gaaggtttta ccgtcaaaag agataaaccc
    10321 tgtatttttg acatgtcgat acttcacaac ctgcataata tctgcacaat aatttttctc
    10381 gaactccttt acctgaatac attcgtcact ggaaatcaga taaggaccga catcgtacaa
    10441 ataagagggg gaaaccgagt cggcgtaagc tgtgcgcaaa ccaaccaaca gctgttcctc
    10501 cgtgggattt agcgtgttga taagatcaat tgccacgcta cgccgatatt cagcaatatt
    10561 tgccaccgca aaagcagtat caataacgga ctgtttcagg ctatacaccc gttcctttac
    10621 catttgagtg tagatcccgc aggcaactgc tgaaatcatg ataaaaatca gcagactgac
    10681 tgtaagtacg aacttattga ttctggacat acgtaaacta ctcttttact aattttccac
    10741 ttttatccca ggcggagaat ggcatgggtt tctggtacag atacccctga gcctgaacac
    10801 atcctaattt aattaattcg cgcgcaacgt cttcgctctc tacgccttcc gcaatcaccg
    10861 ggatccggtt atagcgtgcc agatcgaaaa taatgcgcac aatttttccc gcagtaccga
    10921 aaatatcgcc ggtaaattgc gagtccacct ttatgctgtc tggattaatt tcataaaaac
    10981 gtttcaggtt tgacaaaccg gtgccaaagt catcaatcga tatccgtacc ccttgtttac
    11041 gcagttgttc aatattgaga taaaaatgtt cattaattcg ctcgatcacg gtttcagtga
    11101 tctcaacaca gaggctgttc gccttcaggc cattttcttt catgatggtg gtaaaacgct
    11161 catgaaagtg cggttcgttt aactgccagg gcgaaacatt aatatgcagt aagaaatcat
    11221 ccttcagcga cgcacgctga ctaaaatgtc tgaattcacc cattgccgtt ttaataatct
    11281 gataacccag ctcattgata aacccgatat cttcagcaat ggtaatgaac ttcagcggtg
    11341 aaatgatccc ccgctccgca gaaacccaac ggcacaacgc ctccgcgccc agaatggcgc
    11401 gggtgttaat atcaacgatc ggttgataga cgaggaatag ttcgccaatt tccattgcat
    11461 ggcgaatatt acgcgcaatc cagatattct cttctatttc gttgagtttc gcgtcatcac
    11521 agaaaataaa ctcggagaac atatggtcac ggatactgga aatcgccagt ttcagttttt
    11581 ctgcgtaact ccaggcgttt tccccctgca aaggttcaca aatggcagac ttatggatgc
    11641 gcagattgcg gtagccaaag ctgctgtcaa aatcgtttac catcgctatt ttacgggtaa
    11701 atgtctgtac attttcccga cacagaagta aatagagatc ggcattgaat gtcacgatat
    11761 cgcagcattg cgcatatttc tcggcaattc gctgacacaa tttcctgacg attttattgc
    11821 gttcatcaac accaaacacc gcttccacgg cttccagacc acacagtttg atgatcccca
    11881 cagccagata ctgattattg ttggactcaa taatcgccat atttttaata aaaccgcttt
    11941 cctgatcgta agagatcagt gagagcaatt tatttgaccg ttcaatttcg gcatctttca
    12001 tttccgcgat cgaatttttg atgctgataa tctctttcgc atcgccttcg cgaattttca
    12061 gagcctcgat ctgatccgtt gagtcgggca aaaacgccag cgtattaacc agctcggtca
    12121 tttcagaaaa ttgccgacgg aacacacgag tggtcatgaa atataccacc agcaacacga
    12181 taataatgaa cgcaaacggt agataaaaaa gtttatctga tgcgttcaat aaattaccgc
    12241 caggataata aaaatagatt tcgaacaatg cattatttgg cactgctgat gtctttgcaa
    12301 t
//
LOCUS       DAZGJW010000133        12189 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZGJW010000133 DAZGJW010000000
VERSION     DAZGJW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.133"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..2345)
                     /locus_tag="ACHKIK_003873"
     CDS             complement(<1..2345)
                     /locus_tag="ACHKIK_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9084953.1"
                     /translation="MNDVTVVTSVTYPSPESLALVADVQYHEPYLSAALNRKFRGIVD
                     PGFYAGFLPKPGGGMNLLITSVDGDKTAGAASVDIGEFYQVTIQQCTDIPLALSAGKK
                     YAIVLKGRYLLGEDTYQVNTASHIHAAEFVTRTYTDSYQLGDGELLVCTVNIPAGVSA
                     ITQEMIDTSERINRTIGIDISDSVTSSRSDVAASSLAVKKAYDLANSKYTAQDASTTQ
                     KGLVQLSSETNSDSETMAATPKAVKSVKDLADTKAPIESPSLTGTPTAPTAAQGTNST
                     QIANTAFVKAAITALINGAPGTLDTLKEIAAAINNDPNFSTTINNALALKAPLASPAL
                     TGIPTAPTAAQGTNNTQIATTAYVRAAISALVGSSPEALDTLNELAAALGNDPNFATT
                     MTNALAGKQPLDATLTALAALATGANKLPYFTGKDTVAQTDLTSVGRDILAKTSTQAV
                     IQYLGLRELGTSGEKIPLLSTANTWSARQSFNGGITGALTGNADTATKLKTAININGV
                     RFDGSADININTLVSRGRVTALEANAQGTSGIQLYEAYNNGYPSTYGNVLHLKGATAA
                     GEGELFIGWSGTSGDHAPVHIRSRRDTDSANWSEWAQVYTSKDSIPGVNAKGDQDTSG
                     NAATATKLQTACTINGVSFDGSTDITLTAAHVAAFARRATDTYADADGGVPWNAESGA
                     YNVIRSADSYILVNFYTGVGSCPTLQMKANYRNGGLFYRSSRDGYGFEEDWAEVYTSK
                     NLPPESYPVGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAA"
     gene            complement(2357..2791)
                     /locus_tag="ACHKIK_003874"
     CDS             complement(2357..2791)
                     /locus_tag="ACHKIK_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9084954.1"
                     /translation="MSDVSTNLYKSQLLDYYYQRRAESSINKGSRFLISKAVFGTSSL
                     VTKKGDGTYEIGELPKAFDLAELTSQFCTINLVPTYSGGIITVRMDLDQSQLQEGKNY
                     PFNTLVVLDNENKPIAIICVQEDSLYVGKTYTAVMAINTTTA"
     gene            complement(2870..3706)
                     /locus_tag="ACHKIK_003875"
     CDS             complement(2870..3706)
                     /locus_tag="ACHKIK_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9084955.1"
                     /translation="MQRSWFNNRLTSAKQKSLLYKSLADLVQSMMDTFVDPWLERITN
                     RKSIFSMSKEDLETRTNELGQFFTIRTSDSSSVPMLLQQRLDEIHFKGTERPINQTIY
                     REFNGISVLWDPIYAPVDLERHPYGTVLIPESTLETTGGTFGEMFLTSRGMISIPIND
                     LARTMGITGTIDQSAITEEILRKFNQFVKPLLPLHIVFDGLTLYLSVVVNEQADMITL
                     NEISDTEKAFCWFETSDTTSLTGVTSISAPITATPGGTIVKATPTFDRTRADDLLLDS
                     DA"
     gene            complement(3706..5139)
                     /locus_tag="ACHKIK_003876"
     CDS             complement(3706..5139)
                     /locus_tag="ACHKIK_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin hydrolase"
                     /protein_id="HFO9084956.1"
                     /translation="MSKTTPTKDSIRAEFEELVEKDSFWSKFVGSQFVSMLTLFITQI
                     VYRCFQYADAALAEGFISTATRRSSILAAAETNSYVGTKPTPSSGMVEITATSEDAPA
                     VIPKNTPLISDDQYPYMTMDVCRLVDGTGTVEVAQLEIQEVTYTVTAAKEFLEVVLSK
                     ALTAVCYKLEVFVTTDGKTTQWSSSTMFRLAGSKSQVYVEFYKPSEQLGVRFGDGLIG
                     QIPPEGSTITLKVWCTNGDITLVAGQNLTPVDSAANLANLISVKTTTPITAGTDAETT
                     EITRNRAQYYLAYDDQVVWGGDYTYFLVRNIPGLSWVKAWGEGQQEKLDGAYNVRNIN
                     KIFISGWHPNKSQSELEEMILAAFKKVPNELNKKFSYKEVRKLPFKITITGRISASLT
                     IENVTDELKSALETKFGRDSTFFDPNRVGKYILIKKKDVWAFIETLGYFRDFYLEFVE
                     WNESNGFYDFVYLDTENSTFNISYEEE"
     gene            complement(5136..5492)
                     /locus_tag="ACHKIK_003877"
     CDS             complement(5136..5492)
                     /locus_tag="ACHKIK_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084957.1"
                     /translation="MANNNEIDPLLTLELSGVKTYESQEEAWGARLYEWLNTYQGEVY
                     GDPSWGNVLPQFKHEPTNLSHVQIAVEAMLLQKLTVDLPDIPISGLSVAEGDAFDKLK
                     ISIRIRDITITQDVVL"
     gene            complement(5492..8794)
                     /locus_tag="ACHKIK_003878"
     CDS             complement(5492..8794)
                     /locus_tag="ACHKIK_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO9084958.1"
                     /translation="MERKNANIDDVIRTVETASAKELEELAGIREAVEDLKGGRVATV
                     DPVSRSVSALNRTIENSRPDFVAKAPSVDPIVEAMKRLNLGDVPRVVQEDVALQEPQA
                     KSTTRKGKKRRRKAITEDVKAQRTEAAEHAREMFGQKGGAQKSQNQRDARGRFIGKSG
                     SKAAAEDARAERAEKARRKEDDERLNAESGLLKKLSKVAEGIGNPSETRAVDALGYAV
                     AGPLWAAGKELGGISKEVGGSLNGARKSIADVIRGNDDNSRKKGFFRRKSQSSADVVQ
                     VNTQKRTVQELQDQTSEIKEGNDKILSALDQIAKNTGKKKGGLLSKLFSLLGKGAGGI
                     ASLIFGRGALKKVGSMALGALGIKGVGKLGIKAVAKGALRAIPLVGTVAGGIYDAVTG
                     WNDIEAQRRAFGLKSGQDPSFQQKAAYTLANVLDMGGLVSGISSAIGEVLKSLGFEDI
                     GNMLQSFSTESIAQAIDSGITNLETYISNLGDTISTKFDDYTAKIGDAISAWFSDTTK
                     NLNEKLDAIKNFFTVDNLKQVFSDAIDSAIDFIKNPGKYIKEAGSNLWSAAKELSGEV
                     ADAAVQSTPVAWVASKLVNKADAKEVTPELKTPAKESQEDNAPKTEYTPKKANIVTRV
                     VNASLDTAKDSNKTVKETANQIINANAVETGNRALQKIDNAIGQNSSSSSSLNTTGTR
                     NDIQKAADTYNNGNLDVKVGSLGAEGKANLDKLAPYFAELENKYGLPEGTLYAIAATE
                     SGGNPYAKSQTGALGMFQFTGIAREETGLSREDSFNPEKSAEAAALLMSKYLKQANGD
                     LNEAITAYNAGFGTINKWKKGTGDLSKENREYAIKVNTHRARYLGGEIYTPGAGAQGG
                     AQYGVRGPLPDNAVIDQSTGLAFTPGDSPFEKGGLVDKIGNAVGVNDLVNKFMNGRGM
                     RREVVQGTLEERARGKGTATAAGNVYVDTPMPVEEARPVASNSSYFDQLGAQMGIDGL
                     YDKLINARGMRSNNSPQPASTSQVTTAANDLQQPTGRMQIDGQVISDLGGSGAKPTMQ
                     LADNTVSLDGETKRLFAQMTSLLARIEEHTKDSAKGQGTVVKVSTPQPGVMRTVPLSI
                     DDPLMNDYARVD"
     gene            complement(8876..9757)
                     /locus_tag="ACHKIK_003879"
     CDS             complement(8876..9757)
                     /locus_tag="ACHKIK_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="morphogenetic protein"
                     /protein_id="HFO9084959.1"
                     /translation="MLLPLFPLPSRPTELIQFRQPNIADAMRFNSITPEEQEQQTTAY
                     LKALLAEPAKHDPLTWTAQDRITALWWIFTGSRETPVETFTYTCKHCGKEHYYDCDMN
                     ALAEDIQVLEVEPFIDDIEVSVEGVPYQWRIVPLDGWAMEMLEMRRAALPPEDDAEFK
                     EAIVDLRFWEFAYQCELYNDVSGTREEQAERRYETIKRMAIDTEFMKLAAHIRLAHEK
                     LEHGLPCYIDKGEMRLRLPPHKCPNQDTKESTEGAYTRLWVPFRATDFIPQVGIEKLS
                     DLSVQPGFVWGYTDSGR"
     gene            complement(9772..10383)
                     /locus_tag="ACHKIK_003880"
     CDS             complement(9772..10383)
                     /locus_tag="ACHKIK_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9084960.1"
                     /translation="MGLNVASVKSYVSSALTTTLFGSGVGEREVGKLTSIIMNKMLFA
                     QGWQFSVEVDGLEGADFFAKDITYHDYSIEYETIKIGGGNILQPTERSPGQITMMVRD
                     TVDGLVLDWFKTAKSRVINPDGTGNIPSKYLLNVRIYRLLSSGLTKLENEMTVFPVTT
                     GDVTYARDQVTEFKSFPMTFALHSTFNQSSSSLASLLGFSFSL"
     gene            complement(10394..10960)
                     /locus_tag="ACHKIK_003881"
     CDS             complement(10394..10960)
                     /locus_tag="ACHKIK_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084961.1"
                     /translation="MTPRQLLEDVKSRFTPLIADEPALLESLLRKALGTYQDRAGHIK
                     RIRFTDQTCKSLACPADFLALVSVTDHTGDLVYSDVYDGNIELEDTHRAVYPLNVSYL
                     ANLRDMDLDNGEVPPEIIGLLSDYLEVLIAIPNTDRLRRISIAGKLDASNLSDENTLY
                     QRKLDLEEKMSATRAIIPGIVLFSSMLK"
     gene            complement(11041..11580)
                     /locus_tag="ACHKIK_003882"
     CDS             complement(11041..11580)
                     /locus_tag="ACHKIK_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO9084962.1"
                     /translation="MNKIILFLIFSTFSVGTALANSLQSQIAAIAQAENEGRAKEQQA
                     EDARKELIRQQAQAERIRREKAASAAAAREKQRVAAENERRAKREAELANDKKRDQAY
                     EDELRKLQLESMKLELQAKAARVQRENDFIEQELKERAAKTDVIQSEADANRNISTGS
                     KDLLQSEGKAREKKASSWW"
     gene            complement(11584..12096)
                     /locus_tag="ACHKIK_003883"
     CDS             complement(11584..12096)
                     /locus_tag="ACHKIK_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084963.1"
                     /translation="MSKKYTLCALVVSAILLSGCQSSGADYAADVYDTAQLNSKQETK
                     TVNIISVLPAKVKVDNKANKEAAQTFGAVLGAVAGGVAGYNVKGTSTLGAVAGGTGGA
                     ALGAAAGSLVSDKTIVEGVSLTYKEGTKVFTSTQVGKACQFTTGLAVLISTKDNETRI
                     QPNATCPEKK"
BASE COUNT         3006 a         3100 c         2657 g         3426 t
ORIGIN      
        1 gctgcaagtt tcgggtatgc agatttgtca aaagtctgcc cctgcatcag ggcataacca
       61 gacggaacgg tatctgatgg ccacgggatt ggtgcgccga ctgggtagct ttctggtgga
      121 agatttttcg aggtataaac ttctgcccag tcttcctcaa aaccataacc gtctcttgaa
      181 gaacggtaga acagaccacc atttctgtaa ttcgccttca tctgcaaggt cgggcaactt
      241 ccgactccgg tatagaagtt aaccagaata tagctgtcgg cagagcgaat gacattgtaa
      301 gcgcctgatt cggcattcca gggaacgccc ccatccgcat cggcatacgt atccgttgct
      361 cttctggcaa aagcagcaac atgcgcagcg gttaaagtga tatcagtaga accatcaaac
      421 gagacgccat tgatagtaca tgctgtctgc aacttggtcg ctgtagccgc attaccagag
      481 gtgtcctgat cccctttggc attgacgccg ggaattgaat cttttgacgt atagacctgc
      541 gcccattcag accagttggc agaatcagta tcacgccgcg aacggatatg tacgggcgca
      601 tggtcaccgc tcgtgccact ccagccaatg aataactcac cttcgccagc agcggtggca
      661 cctttaaggt gaagcacatt gccataggtg gaagggtagc cattgttgta tgcctcatac
      721 agctgaatcc cggatgttcc ctgtgcattc gcctctaggg ccgttacgcg accgcgagat
      781 accagagtat tgatattaat gtcagccgaa ccatcgaacc tgacgccatt aatgtttatg
      841 gctgttttta atttcgtcgc ggtgtcggcg ttccctgtca gcgccccggt gatcccgccg
      901 ttgaaactct ggcgcgcact ccatgtgtta gccgtgctca acagggggat cttttcaccg
      961 ctggtaccga gttctcttaa accaaggtat tggataacgg cctgtgtgct tgttttagcc
     1021 agaatatcgc gaccgactga cgttaaatca gtctgagcta ccgtatcctt accagtgaaa
     1081 taaggcagtt tgtttgcacc agtcgcaagg gcagcaagcg cggttaaagt tgcatccaga
     1141 ggctgtttgc ctgccagcgc atttgtcatt gttgtcgcaa agttcgggtc attaccaagt
     1201 gctgcggcaa gctcattcag ggtatcaaga gcttctggtg atgaaccaac caatgcggat
     1261 atggcagctc ttacataagc ggtcgtagca atctgcgtgt tattcgtacc ctgtgcagcg
     1321 gtaggcgcag taggtattcc cgttaatgca ggacttgcta aaggagcttt aagagccaga
     1381 gcattgttga tagttgtgct gaaattcggg tcgttattga tcgcagcagc tatttcttta
     1441 agcgtatcca gtgtgccagg tgcaccgttg ataagtgccg ttatagctgc cttaacaaag
     1501 gctgtatttg cgatctgcgt gctatttgta ccttgcgctg ccgtcggcgc ggttggcgtt
     1561 cctgtcagac tcgggctttc tattggcgct ttggtatcag caagatcttt tacagactta
     1621 acggctttag gggtagccgc cattgtttcg ctgtcgctgt tagtttcgct actgagctga
     1681 actaatccct tttgcgttgt gcttgcatcc tgcgccgtat acttactgtt cgccagatcg
     1741 taggcttttt taactgccag cgaacttgca gcaacatcac ttctgctact ggttacagag
     1801 tctgaaatat caatgccgat cgtgcggtta atacgctcgg atgtatcaat catctcctgg
     1861 gtaatggctg atactccagc agggatattc accgtacaaa caagcagctc cccatctcct
     1921 aactgatatg aatcggtata ggttctggta acaaattcag ccgcatgaat atgtgacgcg
     1981 gtattaacct gataggtatc ctctccaagg aggtatcttc ccttcagcac aattgcatat
     2041 ttcttgcctg cactaagtgc aagaggaata tccgtacatt gctgaatagt tacctggtag
     2101 aattcaccaa tatccaccga cgccgcgcct gcggttttat ctccatccac tgaggtgatt
     2161 aacaggttca tcccaccgcc aggcttaggt aagaaaccgg cataaaatcc cgggtcaaca
     2221 atccccctga attttcggtt tagcgcggct gacagatatg gttcgtggta ttgcacatca
     2281 gcaaccagag ccaacgactc gggtgatggg taagtaaccg atgtaacaac tgtaacgtca
     2341 ttcatcaagc atatccttat gctgtagtcg tgtttatggc cataactgcg gtatatgttt
     2401 tgcccacata cagcgagtct tcctggacac aaataatggc gattggcttg ttctcgttat
     2461 ccagaacaac cagagtgttg aatgggtagt ttttcccttc ctgcaactgg ctttgatcaa
     2521 ggtccattcg gacagtaatt atcccacctg agtaggttgg cacgaggttg atggtacaaa
     2581 attggctggt cagttctgcc agatcgaaag cctttggcag ttctccaatc tcataagtgc
     2641 catctccttt cttagtaacc agagaactgg taccgaaaac ggccttgctg attaaaaatc
     2701 gagagccttt gttaatggac gattcagcgc gccgctgata gtaatagtcc aacaactgac
     2761 tcttatagag gtttgttgag acgtcagaca tgattttccc taatcactgt tgtgaagcct
     2821 cattgtaaga gaagtaactt gtcaccccgc cctgcggacg gggtgattgt caggcgtcgc
     2881 tatccagcaa caaatcatct gcgcgggtgc gatcaaacgt aggtgtcgct ttcacaatag
     2941 tgccaccagg cgttgcggtg atcggggcgc taatcgacgt aactccagta agcgaagttg
     3001 tatccgaagt ttcaaaccag cagaatgctt tttcggtatc agaaatctcg ttcaaagtga
     3061 tcatgtcggc ctgttcattt acaacaaccg acaaatagag cgtaagccca tcaaacacta
     3121 tatgcagtgg cagtagaggc tttacgaact gattaaactt tctgagaatt tcttctgtaa
     3181 ttgcggactg atctatcgtg ccagtaatac ccattgtccg ggccaggtcg tttatgggaa
     3241 tactgatcat ccctctggaa gtcagaaaca tctcgccgaa tgtgccgccg gtagtctcca
     3301 atgtgctttc tggtattaga actgtgccat agggatgacg ctcaaggtcc accggtgcat
     3361 atatcggatc ccataaaaca gaaataccgt taaattcgcg gtaaattgtc tggtttatag
     3421 gacgttcagt tcccttaaag tggatctcat caagccgctg ttgtaacaac atcggaacgg
     3481 aagatgagtc cgaggttctg atagtaaaga actggccaag ttcatttgtc ctggtctcca
     3541 gatcctcctt gctcatggag aaaatagact tccggttggt aattcgctcc aaccatgggt
     3601 caacaaaggt gtccatcatt gactgaacca aatcagccaa tgatttatag agcaatgact
     3661 tttgcttagc tgatgtaagc cggttattaa accaggaacg ctgcatcact cctcctcata
     3721 cgaaatatta aaggtggagt tttctgtatc cagataaacg aaatcgtaaa agccgttgga
     3781 ctcattccac tcgacaaatt ccagataaaa gtcgcggaaa taacccagcg tttcgataaa
     3841 cgcccaaacg tcttttttct taatcaggat gtacttcccg acgcggttcg gatcaaagaa
     3901 agttgagtcg cgcccaaatt ttgtttccag tgccgacttc agctcatcgg tcacattctc
     3961 aatggtcagg cttgccgata tccgtccggt gatggttatc ttaaatggta gttttctgac
     4021 ctctttatac gagaatttct tgttcaactc attcggcacc ttcttaaagg cagccaggat
     4081 catttcttca agctctgact ggcttttatt tggatgccac cctgaaataa atatcttatt
     4141 gatattccga acattataag caccatctaa tttctcttgc tggccttcgc cccatgcctt
     4201 tacccatgac agtcccggga tgttacgcac cagaaaatac gtatagtccc cgccccatac
     4261 gacctgatca tcataggcaa ggtaatattg tgcacgatta cgtgtgatct ccgttgtttc
     4321 ggcatcggta cctgcggtta tgggtgtcgt tgtcttaact gaaatcaaat tagctaaatt
     4381 agccgcagaa tcgacaggcg tcagattttg gccagcaacc aaggttatat ctccgttggt
     4441 gcaccatacc ttaagtgtaa tggtcgagcc ttctggcggt atttgcccaa ttagcccatc
     4501 accgaatcga acccccaact gctcggatgg tttataaaac tcaacgtaga cttggctttt
     4561 actaccggct aaccggaaca tagtgctgga agaccactgc gtggtcttac catcggtcgt
     4621 cacgaatact tccagcttat agcagacagc agtgagagcc tttgataaca cgacttccag
     4681 aaattctttg gctgccgtaa cggtatatgt cacctcctgg atttccaact gtgccacttc
     4741 taccgtaccg gtgccgtcaa ccaacctgca tacatccata gtcatgtaag ggtactggtc
     4801 gtcagatatt aaaggcgtgt ttttggggat taccgctgga gcatcttcac ttgtggctgt
     4861 gatctcaacc atcccggatg acggtgttgg cttggtacca acgtaactat tcgtttctgc
     4921 cgctgccagg atagaagaac gccgcgtcgc ggtcgatata aagccttcag ccagcgccgc
     4981 atcggcatac tgaaagcacc tgtagacaat ctgggtaata aacaatgtca gcatcgagac
     5041 aaattgagag ccgacaaact tcgaccagaa tgaatctttc tcgacaagct cttcaaactc
     5101 tgcacgaata ctgtctttag tcggtgttgt tttactcata gcaccacgtc ctgtgtgata
     5161 gttatatccc tgatacgaat ggatattttc aacttatcaa aagcatctcc ctcggctact
     5221 gacaagccag aaatcggtat gtcaggtaaa tctaccgtca gtttttgcaa cagcattgcc
     5281 tcaaccgcaa tttgaacatg cgacaagttg gtcggttcgt gtttaaactg cggtaaaaca
     5341 ttgccccatg acggatctcc gtatacctca ccctgataag tgtttagcca ctcatataaa
     5401 cgagcgcccc aggcctcctc ctgggactca tacgttttta cgccggataa ctccagcgtc
     5461 agcaaaggat caatttcgtt attgttggcc atcaatcaac tctcgcgtag tcattcatca
     5521 acggatcatc aattgacagt ggtaccgtgc gcataacgcc cggctgaggc gtgctgacct
     5581 ttacgacagt tccctggcct ttcgccgagt ctttggtgtg ctcttcaatc ctggcaagca
     5641 atgaggtcat ctgcgcaaac agccgcttcg tttcaccatc aagtgaaacg gtattatcag
     5701 ccaactgcat tgtcggcttg gcaccggagc cgccaaggtc actaataacc tgtccgtcta
     5761 tctgcatacg accggttggt tgctgcaaat cgttggcggc agtcgtcacc tgggacgtgg
     5821 aggctggttg aggagaatta tttgagcgca ttccccgggc attaatgagt ttgtcataca
     5881 gtccatcaat ccccatttgt gcgccgagtt ggtcaaagta acttgagttg ctggccaccg
     5941 gacgcgcctc ttcaactggc atcggagtat caacatacac attgccagct gctgttgcgg
     6001 tccccttccc tcgtgcacgt tcttcgagcg ttccctgaac gacttcccga cgcatacccc
     6061 ggccattcat gaatttgttg accagatcgt taacgccaac agcattgcca attttgtcta
     6121 ccagaccgcc tttctcaaac gggctatcac caggggtaaa cgccaggcca gtagactgat
     6181 cgataacagc gttatcaggc agtggtcccc tcactccata ttgcgcccca ccctgtgctc
     6241 ctgctccagg tgtatagatt tcaccaccta aatagcgagc acgatgagta ttgaccttga
     6301 tcgcgtactc acggttttct ttcgataagt cacctgtgcc ttttttccac ttattgatag
     6361 tgccaaaccc agcgttatat gcagtgatgg cctcgtttaa gtctccattg gcttgcttca
     6421 gatacttgct catgagaaga gccgcagctt ctgccgattt ttccggatta aaagaatctt
     6481 cccgggataa tccagtttcc tcacgagcaa tccccgtgaa ctgaaacatt cccagagcac
     6541 cggtttggga ttttgcatac ggattaccac cagattcagt tgcagcgatc gcgtaaagag
     6601 taccttcagg aaggccatat ttattctcta gttcagcaaa atacggagcc aacttatcga
     6661 gatttgcctt accttcagcg ccaaggcttc cgacttttac atctaagttg ccattgttgt
     6721 aggtatccgc agctttctga atgtcattcc tggtgccagt agtattaagc gacgatgatg
     6781 atgagctatt ttgaccaata gcattatcaa ttttctgcaa cgcgcgattg cccgtttcta
     6841 cggcatttgc attgataatc tgattggcag tttctttaac tgttttattg ctatctttcg
     6901 ccgtatctag ggatgcattt acaacacgag tgacaatatt agccttttta ggggtatatt
     6961 cagtcttagg agcattgtcc tcctgacttt ctttagctgg tgtttttaat tcaggtgtaa
     7021 cctctttcgc atccgctttg ttgactagct ttgatgcaac ccaggccaca ggggtgctct
     7081 gaacagcggc atctgcaacc tcacctgaaa gctcttttgc ggcactccat agattactgc
     7141 cagcctcttt aatgtatttc ccaggattct taatgaaatc aattgcacta tcaattgcat
     7201 cactgaaaac ctgtttcagg ttatcgacag taaagaagtt tttgatggcg tctaattttt
     7261 cgttcagatt ctttgtggtg tcactgaacc atgcagaaat agcatcacca atctttgctg
     7321 tataatcatc gaacttggta gaaatggtgt cgccaaggtt agaaatatat gtttctaagt
     7381 tggtaatccc actatcaatg gcctgggcaa tactttccgt cgaaaatgat tgcaacatat
     7441 tgccgatatc ctcaaatcca agtgatttga gaacctcacc aatggcgctg ctaataccag
     7501 ataccagtcc ccccatatca agaacattag ctaacgtata agcggctttt tgctggaatg
     7561 atggatcttg tcctgattta agcccaaacg ctcggcgttg cgcttctata tcattccaac
     7621 cggttaccgc atcatagata cctccagcca ccgtgccgac tagcggaatt gcacgtaaag
     7681 cgccttttgc gaccgcctta atccctagtt tccctacccc cttaatacct aaagcgccta
     7741 gtgccatcga gccaactttc tttagtgccc cacggccaaa aatgagggaa gcaataccac
     7801 cggccccctt ccctaacagg ctaaataatt tggacagcaa gccgcccttc tttttcccgg
     7861 tgtttttggc tatctgatca agggcgctga gaatcttgtc attgccctct ttaatttcgc
     7921 tggtctgatc ctgaagttcc tgaaccgtcc gtttttgggt gttaacctga acgacatcgg
     7981 cactactttg cgatttacgc ctaaaaaaac cttttttacg gctgttatcg tcattgccac
     8041 gaatcacatc ggcaatagac tttctggcac cattaagcga tccgccaact tcttttgata
     8101 tcccgccaag ctccttccct gctgcccaca atggaccagc aacggcataa cctaacgcat
     8161 cgacggcacg agtctctgaa gggttaccta tgccttcagc tacttttgac agttttttta
     8221 ataaacctga ttcagcattt agacgctcat catcctcttt gcgcctggcc ttttcagcac
     8281 gttcagcacg ggcatcttcc gctgcggcct tactccctga ctttccaata aaacgaccac
     8341 gcgcatcgcg ttggttttgg cttttttgcg caccgccttt ttgaccgaac atttcgcggg
     8401 cgtgttcggc tgcttcggtc cgttgcgcct ttacatcttc tgttatagcc ttcctgcgtc
     8461 gttttttacc ctttcgcgta gttgatttgg cctgcggttc ctgtagagca acatcctcct
     8521 gaactacacg aggaacgtcc cctaaattaa gccgtttcat tgcctcaaca atagggtcca
     8581 ctgatggcgc tttggccaca aagtcaggcc gggaattttc gattgtgcga tttaatgccg
     8641 acacactgcg agagacagga tcaactgtag caactcgccc ccctttcaaa tcttcaacgg
     8701 cttcccgaat acctgcaagc tcttccagct cttttgcgct ggcggtttca actgtcctta
     8761 tcacatcgtc aatgttggcg ttttttcttt ccatgatctt atcgcctacc gcttcggttt
     8821 aagtttttct tccagtttct ccaacaggaa aaacgcatag gattcagtga gcctttcagc
     8881 gtcctgaatc ggtatacccc catacaaaac caggttggac actaaggtct gatagctttt
     8941 caatccccac ctgtggaatg aagtcggtag cccgaaaggg cacccacaga cgggtatacg
     9001 caccctctgt ggactccttt gtatcctggt ttgggcattt gtgcggcggg agacgaagac
     9061 gcatttcgcc tttatcgatg tagcacggta aaccatgttc gagcttttca tgagccagtc
     9121 ggatgtgtgc cgccagcttc ataaattcag tatcaatggc catccgtttg atcgtttcat
     9181 aacgacgctc agcctgctct tcacgagtac cgctaacatc gttataaagt tcacactgat
     9241 aagcgaattc ccaaaaacgc aaatcaacga tcgcttcttt gaattccgcg tcgtcttcag
     9301 gtggcaatgc tgcacggcgc atctccagca tttccattgc ccaaccatca agcggcacga
     9361 tacgccattg ataaggcacg ccctctacag acacctcaat atcgtcaatg aacggttcca
     9421 cttccaggac ctggatatct tcagccagag cattcatatc gcaatcgtaa taatgctctt
     9481 taccgcaatg tttacaggtg taggtgaatg tctcgaccgg tgtttcacgg gagccagtaa
     9541 atatccacca taacgcggta atccggtcct gcgccgtcca tgtcagggga tcatgtttcg
     9601 cgggttcagc cagcaaggct tttaaatacg ccgttgtctg ttgttcttgt tcctccggtg
     9661 ttatcgagtt gaaacgcatc gcatcagcaa tatttggctg acggaactgg atcaattcag
     9721 ttggccgcga tggtagcggg aaaaggggta aaagcatcct tgctccttaa ttcaaagaga
     9781 aaaactaaag cccagaaggg aagccaaaga acttgaggat tggttaaacg tgctgtgcaa
     9841 tgcgaaggtc attgggaatg acttaaattc cgtaacctga tcccgcgcat aggtgacatc
     9901 tccggtagtg accgggaata ccgtcatctc attttccagt ttggttaagc cggaagacag
     9961 caaccgataa atacgcacat tgagcaaata tttagacggt atattcccgg taccgtccgg
    10021 attgatcacc cgactttttg ccgtcttaaa ccagtccaaa acgaggccat caacggtatc
    10081 cctgaccatc attgttatct gcccaggcga acgctccgtt ggttgaagga tattccctcc
    10141 gccgatttta atcgtttcat attcgatgct gtaatcgtgg taggtaatat ctttggcaaa
    10201 gaagtctgcc ccctccagtc catcaacttc gacagagaac tgccatcctt gcgcgaacag
    10261 cattttgttc atgatgattg acgtcagctt accaacttcc cgctcaccaa cgccggagcc
    10321 aaataatgtc gtcgttaatg ccgaagatac ataagacttt actgaagcaa cattaagccc
    10381 catatcagcc ccctcacttc aacatggatg agaaaagaac aattcccggg ataattgccc
    10441 ttgttgcgct cattttctct tccagatcca gctttcgctg atacagcgtg ttttcgtcgg
    10501 ataaattgct ggcatcgagt ttccccgcga tagatattct tcgcaggcga tcagtgttag
    10561 gtattgcgat tagcacttcc agatagtcag aaagtaaccc aatgatttca ggtggcactt
    10621 ccccattatc cagatccata tcacgcaaat tagccagata tgacacattc agtgggtata
    10681 ccgctcgatg ggtatcttca agctcgatat tcccatcgta aacgtcggag tagacaagat
    10741 cgccggtatg atccgtaacc gatacgagcg caagaaaatc agcagggcaa gcaagtgatt
    10801 tacaggtctg atcagtgaag cgtatccgct tgatgtgtcc cgccctatcc tggtaggttc
    10861 ccaatgcttt tcttagcagg gattccagta aggcaggttc atccgcaatc aaaggtgtga
    10921 agcgggattt gacgtcttcg agtaattgtc gtggtgtcat tgaaacctcg tagaatctgg
    10981 tttgttaaca gattctacga gttgtcattt gtgacagtat gctcgcaatc gtagtgatta
    11041 ctaccaccaa ctgctagctt tcttctctct agcttttcct tcgctttgca gtaaatcttt
    11101 acttcctgta gaaatatttc tatttgcatc agcctcagac tgaattacat ctgtcttagc
    11161 tgctctctcc ttcaactcct gctctataaa atcgttttct cgctggacac gagccgcttt
    11221 tgcttgcagt tcgagtttca tgctttcgag ttgcagcttg cgaagctcat cctcataagc
    11281 ttgatctcgc ttcttgtcat ttgctagctc ggcttctcgt ttcgctctac gttcattttc
    11341 tgcagcaaca cgctgttttt cgcgtgcagc agcggcagat gctgcttttt ctcttctaat
    11401 acgttcagct tgtgcttgtt ggcgaataag ttcttttctg gcatcctcag cttgctgctc
    11461 tttagctcgc ccttcatttt ccgcttgagc aatagcagca atttgacttt gcaacgagtt
    11521 agccaaggca gtgcctacac taaacgtaga aaagattaga aataaaataa ttttattcat
    11581 aaattatttc ttttctggac aagtagcatt tggctgaatt cgagtttcat tatctttagt
    11641 tgaaataagc acagcaagcc ccgttgtaaa ctggcatgct ttaccaactt gagttgaagt
    11701 gaacacctta gttccttctt tataagtcag tgaaacacct tcaacaattg ttttatcact
    11761 aaccaaagaa ccagccgctg caccaagagc cgcaccacca gtaccgccag ctacagcacc
    11821 cagtgtcgat gtgcctttaa cattataacc ggcaacgccc ccagcaacag ctcccagtac
    11881 cgcaccaaat gtctgtgccg cttctttatt agctttatta tcaactttca ctttagctgg
    11941 aagcacagag ataatattga ctgtctttgt ttcctgcttc gaattcaatt gagcggtatc
    12001 ataaacatcg gcagcataat cagcaccgct agattgacac ccagaaagaa gaattgcaga
    12061 tacaacaaga gcgcataaag tatacttttt agacataaga atcattccag aaaaacaaaa
    12121 accggcgaga agttaccacg agaacggaaa gtaaacaaga atactcccat tgacttatat
    12181 caattaatc
//
LOCUS       DAZGJW010000134        12142 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZGJW010000134 DAZGJW010000000
VERSION     DAZGJW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.134"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(36..938)
                     /gene="yihR"
                     /locus_tag="ACHKIK_003884"
     CDS             complement(36..938)
                     /gene="yihR"
                     /locus_tag="ACHKIK_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO9084964.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            complement(1076..2317)
                     /gene="yihS"
                     /locus_tag="ACHKIK_003885"
     CDS             complement(1076..2317)
                     /gene="yihS"
                     /locus_tag="ACHKIK_003885"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO9084965.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(2334..3212)
                     /gene="yihT"
                     /locus_tag="ACHKIK_003886"
     CDS             complement(2334..3212)
                     /gene="yihT"
                     /locus_tag="ACHKIK_003886"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO9084966.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(3236..4132)
                     /gene="yihU"
                     /locus_tag="ACHKIK_003887"
     CDS             complement(3236..4132)
                     /gene="yihU"
                     /locus_tag="ACHKIK_003887"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO9084967.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            4300..5196
                     /gene="yihV"
                     /locus_tag="ACHKIK_003888"
     CDS             4300..5196
                     /gene="yihV"
                     /locus_tag="ACHKIK_003888"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO9084968.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5230..6015
                     /gene="csqR"
                     /locus_tag="ACHKIK_003889"
     CDS             5230..6015
                     /gene="csqR"
                     /locus_tag="ACHKIK_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO9084969.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            6114..6713
                     /gene="yihX"
                     /locus_tag="ACHKIK_003890"
     CDS             6114..6713
                     /gene="yihX"
                     /locus_tag="ACHKIK_003890"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO9084970.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            6707..7579
                     /gene="yihY"
                     /locus_tag="ACHKIK_003891"
     CDS             6707..7579
                     /gene="yihY"
                     /locus_tag="ACHKIK_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO9084971.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            7576..8013
                     /gene="dtd"
                     /locus_tag="ACHKIK_003892"
     CDS             7576..8013
                     /gene="dtd"
                     /locus_tag="ACHKIK_003892"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO9084972.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            8058..8999
                     /gene="fabY"
                     /locus_tag="ACHKIK_003893"
                     /gene_synonym="yiiD"
     CDS             8058..8999
                     /gene="fabY"
                     /locus_tag="ACHKIK_003893"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO9084973.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(9063..9971)
                     /locus_tag="ACHKIK_003894"
     CDS             complement(9063..9971)
                     /locus_tag="ACHKIK_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9084974.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            10200..10511
                     /locus_tag="ACHKIK_003895"
     CDS             10200..10511
                     /locus_tag="ACHKIK_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9084975.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            10512..10802
                     /gene="nadS"
                     /locus_tag="ACHKIK_003896"
     CDS             10512..10802
                     /gene="nadS"
                     /locus_tag="ACHKIK_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO9084976.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            11407..11625
                     /gene="yiiE"
                     /locus_tag="ACHKIK_003897"
     CDS             11407..11625
                     /gene="yiiE"
                     /locus_tag="ACHKIK_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO9084977.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            11844..12086
                     /gene="yiiF"
                     /locus_tag="ACHKIK_003898"
     CDS             11844..12086
                     /gene="yiiF"
                     /locus_tag="ACHKIK_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFO9084978.1"
                     /translation="MNSLAGIDMGRILLNLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
BASE COUNT         2977 a         2939 c         3201 g         3025 t
ORIGIN      
        1 gttcttcaat gattttgact gctaatcaat acaagttatt ttctctgtcc gtaaatgtta
       61 aaaaacaggc ggtgcggttt tcctggttcc agtaataata agtcgatacc tgaattaaag
      121 gcgttaggcg ggcaactcat aggttcaact gccagtccct gacgctgtaa tttttcaccg
      181 ctataaattt gtacccaggg ttgatcgcaa caaaggctaa ccgaaagcgc ctgttgtgga
      241 tgggtaatgg ttatttccca cagatcatta gcggttttaa aggtgtggtc gatttttgtc
      301 gcggctattg ttttcgcctg cgtgaaattt aaatccagct catcaacgtg gtgaagcgtt
      361 gtcggattta cgtgttcatc aacggcataa acctggtttg ccggaagctg gaacagatat
      421 tcatcaacag atgttaagtt gcaggttaaa taagtatgaa tacccacgcc ataaggcgct
      481 gcgacagtgc cgatattctg gcttgcgatc tcaacggaaa gacccgtgtg tgcgttgagc
      541 gaataaacaa cttgtgaagc cagcataaac ggatagccgt aactgggcgg tagaaaagct
      601 gtcagggtaa cgctggtggc ggttaattca ctaatttgcc agtctcgcca ggcaagcagc
      661 ccgtgaatag cggcttttga actatgttca ttaatgggta actgatattc ctgcccctga
      721 tagcggtaac agccgttggc gatccggttc ggccacggaa taagcacttt acccagatgc
      781 gccagcggca tctcttcggg tttgtgcggg atgactaaat gacaaccctg aaaggttaat
      841 tcagccagac cagcaccgac ggtcacaatg gtcgcgtgat aatcccccgc ggcaagtgaa
      901 acggtctgtc cactgcaatg catattggtg atttgcatac aaggcacctt aattaacgac
      961 atacagcctc ccgtgaggct gatgtcaaac ccaaaaactg caccggacgg tcccctcgcc
     1021 cctttgggga gagggttagg gtgaggggaa aacccgcacc ctattttcag atcagttatt
     1081 tcgcattaat atccagcaga cccgctgcaa ccgctggagc catgccgggc gctaacggga
     1141 tacgcgggat caccaggcaa tgcagcaggt gataaatatc ctgtttgccg tcccagactt
     1201 tggtggtgac cttattgtcc gcatccagct cctgccacca ggaaccattt tcatagtcca
     1261 tcaggtactt aatgcagtac tcccaccatg tttgatacca ggtttcatac tggcgatcgc
     1321 cagtgacggt gtagagcgcg taggccgtac ccattgcttc gacgataggc caacgtacac
     1381 gttcgcggac caccggtttt ccttcccagt caacggtata aacaatcccg tccgcgccat
     1441 cgggtgccca ggcatcgcgc acggtggcgt taaacagacc tttggcatct tctagcagcc
     1501 atgctggtgg ttgttcgcaa cgggcttcca gggcagcatg gatgtgcagc attaaacggc
     1561 cccattcgat ccagtggcct ggtgtacctc cgaacgcgcg gaagcgatgc gccgggttat
     1621 ctttgttgta atccggcagc ggattccact gggtatcgaa gtgttcgtta acgcgataat
     1681 gattatttct ggcgacgtcg tggataatca cggaagccac gcgaatcgcg cgatccagcc
     1741 attttttgtc gtgagtgacg tcataaacaa tcaagaaagc ttccaccgcg tgcatattgg
     1801 cattgccgcc gcggtactct tcggttttgc tgaaggcttc gtcccaggat tccaggcaca
     1861 tctgctcttc ttcgctccag aaatatttct cgataatttc aatggtgtaa tcgagcagct
     1921 tgcgcgcttc cgggtgaccc gttgtgacgg cgctggcggc acccagcaga gcaaagaagt
     1981 gttgatagcc ctgtttggag gcatccacca cgccctcgtc atttacgcag gcataccagc
     2041 cgccatattt tttatcacgc agtgcgccgt tcatggcttt gataccgtga tcaaccaacg
     2101 agtaagcgcc aggtcgaccc atcgccgcag caacggaata aacgtgtaac atacgagcgg
     2161 tgatccacag atgggtgccc atctcttctt tgatttgccc tttattgcct aaccagccaa
     2221 aaccagtcgg cactactgaa tttttaccaa aatcaaagat gcggtcggtt tcctgttcca
     2281 gccaacggtt gtggcttagg gtgttaaacc atttcattcc ttttatcctc atcttagcgg
     2341 cgtttagcca tcatttcgtc gacgatatcg ccaagttgtt gtaatttcgg tgcgcaaacg
     2401 tcacgcagca tcagctcgtt gtctggtaaa ccgacgaccg atgcccagac tgcacggcct
     2461 gccaggaatc ccgatgcgcc cgccgtcatt gccacgcgta cggcacgcgg gaacagtttt
     2521 tcgtcgacac cggaagagag gatcacccat ggcatattga tatggtcatt cagacgttgt
     2581 gaagcacaga gaagctcttg ttgcggacct ttgccataaa gcggcatttc aactttgtag
     2641 aggtcagcgc cactgtcgcc cagctctttg gcggcatcga tgatcgcttg ttcgcgatcg
     2701 aatttatcgc cacgacgcgg tgggcggacg actggctcaa tgatgcttac cagaccgtgt
     2761 gagtggcaca gttcgttgaa ctctttcacc atatccagac gttgctgcgc atcttcatca
     2821 ctacgccaca gcaccagcag ttttaaggct ttgccaccgt cttgtttgat ctgtagcgga
     2881 ttgattttgc ggtcaataac cacgctatcg accggaatac cgttgccagg aatgaactca
     2941 tcggcggcga caatcatggc gcaacttttg gcaatcgcgt tttgctcaac cacctggcgg
     3001 tagcagaatt gttgatctac cagaatcgcc gaggcataag gcgagagggc ctttgcagcg
     3061 ttaactttga aatcagttaa aacgctatcg gctaccggag cgggtgcccc agccgcggca
     3121 aacatcatgc gcatggcttc gcgctgatcg accgccagca tggcaaaacc gcccgatgcg
     3181 cgcgtaatgt cgttgatggt gtacttattc attcatttat tcctttatcc agtcgttaca
     3241 tttttacttt ggcagtcatc ccggcactga cacggacctg ttccagaatg gcggaccagt
     3301 cctggcgacc gcgacccgct gcgcgcgcct ggctataaac ctcccgtgag gcggccccca
     3361 gcggcattgg cacatgcagc tggttggcga catcaagggc gatgccaaga tccttatggg
     3421 caagatcgat catgaaggcg ggagaaagat ctccgctgag gactttgttt ggccaggaag
     3481 tggtgaagtg acctttaccg gcggcggtac cgctcatcac tttgacggca acatcgaagg
     3541 gaagattcag ggcttcgcac aaaacggcgg cttctgccga aagcgcattg agcgcgatgc
     3601 tcatgtagtt gttgatgagc ttaacgcgga tccccatgcc cggaccgcca gcgttgatca
     3661 actcactgcc catcgccatc aggatcggtg tggcacgttc aacttgttca gcggtgccgc
     3721 cagccagcag taacagagta ccggtaatgg catttgcaga agtacggcct accggaacat
     3781 ccatcatgct gaagcctttg gcttgcatat cggcaatcaa tttatcggtt tgcagcggat
     3841 ggatggtgga catatcaatg accagcgcat cggtagataa gccttcgcaa acgccgtttt
     3901 caccgaacaa cacgttgcgc accagatcgc cattcggcaa catggtaatg ataaattcgg
     3961 catctttagc ggcctgcgcc gggttggcgg cgggagtcgc acctttgtct accagatgcc
     4021 gcacagcttc ggcgttcaca tcaaagacgc gaagttgatg cccttgctgc aataaattgc
     4081 tcgccattgg cgaacccatt tgtcctaaac cgataaacgc gattgctgcc ataaccctct
     4141 cctgaataca gtgatgtcac tttttgtcat ttatgacatg ctttgcttgt ctgtttttga
     4201 tcgtatttgt aatttatcgt caaaaaattg acagccgtca ctttttaaac aattggtgaa
     4261 attaaaatga acgcatccca aaatgtttaa ggaatgacca tgattcgtgt tgcttgtgta
     4321 ggtataaccg tgatggatcg catctattac gtggaagggt taccgacgga gagcggtaaa
     4381 tacgtggcga gaaattatac ggaagttggt ggcgggccag cggcgacggc agcggttgcg
     4441 gcggcaaggc tgggggcgca ggtcgatttt attggtcgcg taggtgatga cgacacgggc
     4501 aacagcctgc tggcagagct ggaatcctgg ggcgttaaca cccgttacac caaacggtat
     4561 aaccaggcga aatcttcgca atccgccatc atggtggata ccaaaggcga gcggataatc
     4621 attaactacc ccagcccgga tctgctgcct gacgcagagt ggttggagga aattgatttc
     4681 tctcagtggg atgttgtgct ggcagatgta cgctggcacg acggcgctaa aaaagccttc
     4741 accctggccc gtcaggcggg tgtgatgacc gttctggacg gggacattac gccgcaggat
     4801 atcagtgagc tggtggcatt aagcgatcac gcggcctttt cagaaccggg tctggcgcgc
     4861 ttaacgggcg tgaaagagat ggccagtgcg ctaaaacagg cacaaacgct cacaaatggt
     4921 catgtctatg tgacccaggg tagcgcaggc tgcgactggc tggaaaatgg tgggcgtcag
     4981 catcaaccgg ccttcaaagt tgatgtggta gataccacag gtgcgggtga tgtttttcac
     5041 ggcgctttgg cggtggcgct ggcaacaagt ggggatttag cggagtcagt ccgcttcgcc
     5101 agcggtgtag cggcgttaaa atgcacacgt cccggtggac gagccgggat ccctgactgt
     5161 gatcaaaccc gatctttttt gtcacttttt gtataaaatg ccagggtgat ggtttttcga
     5221 ggaattttca tgagccttac cgaactaacc ggtaacccgc ggcacgacca actcctcatg
     5281 ctgatcgccg agcgtgggta tatgaatatt gatgagctgg caaatctgct ggatgtctcc
     5341 acgcagacgg tccgccggga tattcgtaaa ttaagcgagc aaggcctgat tacgcgccat
     5401 cacggtggcg cgggtcgggc ttccagcgtc gttaatacgg cgttcgagca gcgtgaggtt
     5461 tcgcaaaccg aggaaaaaaa agcgattgcc gaagcggtgg cagactatat tcctgatggt
     5521 tcaacaatat ttatcaccat tggtacgact gttgagcatg ttgcccgggc gttacttaac
     5581 cataatcatt tgcggataat caccaacagc ctgcgtgtgg cgcatattct ttaccacaac
     5641 ccgcgctttg aagtgatggt gcccggcggt acgttgcgct ctcataatag cgggatcatt
     5701 ggcccttcag cggcgtcctt tgtggctgat tttcgtgctg attatctggt aacaagcgtt
     5761 ggggcgattg agagcgatgg cgcgttgatg gagtttgatg taaacgaagc taacgtggtg
     5821 aaaacgatga tggcgcacgc gagaaatatt ctgctggtcg ccgatcacac taagtatcat
     5881 gcttcggcgg cggttgaaat tggtaacgtg gcacaggtca ctgcgctctt taccgacgag
     5941 ctgccgcccg ctgcgctaaa atcacgctta caagacagcc aaattgaaat cattcttccc
     6001 caggaagacg cgtagatttt tgtgaccgtt aacctggctt cataccttgc cacatagcca
     6061 aacccatcct ttcccgctac agttaatttc ttgtggcgcg aaaggaggca aaaatgctct
     6121 atatctttga tttaggtaat gtgattgtcg atatcgactt taaccgtgtg ctgggagcct
     6181 ggagcgattt aacgcgtgtt ccactggcta cgcttaagaa gagtttccac atgggggagg
     6241 cgtttcatca gcatgagcgt ggggaaatta gcgacgaagc gttcgcagag gcgctgtgtc
     6301 atgagatggc cctgccgcta agctacgagc agttctctca cggctggcag gcggtgtttg
     6361 ttgcgctgcg cccggaagtg atcgccatca tgcataaact gcgtgagcag gggcatcgcg
     6421 tggtggtgct ttccaatact aaccgcctgc ataccacctt ctggccggaa gaatacccgg
     6481 aaattcgtga tgctgctgac catatctatc tgtcgcaaga tctggggatg cgcaaacctg
     6541 aagcacgaat ttaccagcat gttttgcagg cggaaggttt ttcacccagc gatacggtct
     6601 ttttcgacga taacgccgat aatatagaag gagccaatca gctgggcatt accagtattc
     6661 tggtgaaaga taaaaccacc atcccggact atttcgcgaa ggtgttatgc taaaaaccat
     6721 tcaggacaaa gccaggcacc gtacccgtcc actatgggcc tggctaaaac tactctggca
     6781 acgtattgat gaggacaaca tgacaaccct ggcaggtaac cttgcctatg tgtcgttgct
     6841 ctcattagtg ccgctggttg ccgttgtttt tgcgcttttc gccgcttttc ccatgttttc
     6901 cgacgtcagc attcagttac gtcactttat ttttgccaac tttctgcctg ctactggcga
     6961 tgttattcag cggtatatcg aacaatttgt tgccaattcc aacaagatga ccgccgttgg
     7021 ggcgtgcggg ctgatcgtca ccgcgttatt gctgatgtac tccatcgata gcgcgttgaa
     7081 taccatctgg cgcagtaaac gagcgcgacc aaaaatttac tcgttcgccg tgtactggat
     7141 gattttaacg ctggggccgc tgctggcagg ggccagtctg gcgatcagtt cctatttgct
     7201 ctccctgcgc tgggcgagcg atctcaatac tgtcatcgat aacgtgctgc gtatttttcc
     7261 gctgctgttg tcgtggatct ccttctggtt gctgtacagc attgttccta ccatccgcgt
     7321 acctaaccgc gacgcgattg tcggcgcgtt tgtcgccgcg ctcctgttcg aagcaggaaa
     7381 gaaaggtttc gcgctttata tcaccatgtt cccgtcatat cagctcattt acggcgtgtt
     7441 ggcggtgatc cccattctct ttgtttgggt ctactggaca tggtgtatcg tcttgcttgg
     7501 cgcggaaatt actgtcactc tcggggaata ccgcaaacta aaacaagcag ctgaacaaga
     7561 agaagacgac gaaccatgat tgcattaatt caacgcgtaa cccgtgccag cgtcaccgtg
     7621 gagggagaag tgacgggcga aattggcgcg ggacttttgg tgttattggg tgtcgaaaag
     7681 gatgacgacg aacagaaagc aaaccgtctg tgcgagcgtg tgctcggcta ccgcatcttt
     7741 agcgatgccg aaggcaagat gaatctcaac gtgcaacagg cgggcggcag tgtactggtg
     7801 gtttcccagt ttaccctcgc cgcagatacc gaacggggga tgcgcccaag tttctctaaa
     7861 ggcgcatcac cggatcgcgc agaggcgtta tatgattatt tcgtcgaacg ctgccgccag
     7921 caagagatga acacgcaaac aggacgcttc gctgccgata tgcaggtatc gctggtcaat
     7981 gatggccccg tgacattctg gttgcaggta tgagccagct tccagggttg tcacgggaaa
     8041 caagagagag tatcgctatg tatcaccttc gggttccaca aacagaagaa gagttagagc
     8101 gttactatca gtttcgctgg gaaatgttgc gtaagcccct gcatcaacca aaaggttcgg
     8161 aacgcgacgc gtgggatgcg atggcgcatc accagatggt cgtcgacgag cagggtaatc
     8221 tggtggcggt aggccgattg tatattaatg ccgacaatga agcgtccatt cgctttatgg
     8281 ccgttcaccc ggacgtgcag gacaaagggt taggaacgct gatggcgatg accctggagt
     8341 cggtggcgcg tcaggaaggc gttaagcgcg tgacctgtag cgcccgtgaa gacgcggtgg
     8401 agtttttcgc caagctgggg tttgttaatc agggagaaat caccacgcca accaccacgc
     8461 cgattcgcca ttttttgatg attaagcccg tcgccactct ggatgatatt ttgcatcgcg
     8521 gcgactggtg cgcgcagctg caacaggcgt ggtacgaaca tatcccgctt agtgaaaaaa
     8581 tgggcgtgcg tattcaacaa tataccgggc aaaaatttat caccaccatg ccggaaaccg
     8641 gtaatcagaa tccgcaccat acgctgtttg ccgggagttt attctcactg gcaacgctca
     8701 ccggttgggg gcttatctgg ctgatgctgc gcgaacgcca tctcggcgga acgattattc
     8761 tggcggatgc gcatatccgc tacagcaagc cgattagcgg taaacctcat gcggtagccg
     8821 accttggtgc cttaagcggc gatctcgacc gtctggcgcg cggacgaaaa gcacgggtgc
     8881 agatgcaagt tgaaatcttt ggcgacgaga cgccgggtgc agtgtttgaa ggcacgtata
     8941 tcgttctgcc cgcgaagcca tttggcccgt atgaagaggg cgggaacgaa gaagagtagg
     9001 ggcgttttac ttgactgaaa aggttttcaa tctgaaaacc ttttcagggc ccaggtccga
     9061 agttaccttc ccgctctcca ggcaacatcc cgtagtgccg atcgcgcggc ctcactgata
     9121 ccacctaact gaaaaaatcc gtggataacg ccaaggtagc gttggcaggt gcattgcact
     9181 ccttgccctg tcatgcggtg atacaacgct tcaccttcat cgcaaagtgg gtcgtattca
     9241 gcggtaatga tatgtacagg tggcaagcca ttgaaatcat cgcgccaaag agggctggct
     9301 tcaggatgcc tgcgttcaat gcctggcaga tacatctcaa agccgctcag taaagtgtcg
     9361 cgtgtgatga tgtaatcgtg accattgcgt atgtagcttt cgaaatgcgc agtcgcatca
     9421 agcataggat aaattaagat gagttgtgcc ggttgccact cgccagcacg ctttaatcgc
     9481 aaagcagtta ccagcgccag atgtccgccc gcactgtcac cggcaagagt tatacgttgt
     9541 ttatcaacgc cgagtcgttc ggcgtgttgc cagactattt ctgcacctcg ttgcgcatca
     9601 tcatgggcag cggggaaagt atgttccggt gccagccgat actggacggc aataacccga
     9661 cattgcccgt aataggctaa ctggcgcagc tggttgtcgt gagttgcgaa cccgccgcta
     9721 acaaaacagc caccgtagta ataaattatc gtcgggagta atgtcggggc attcagcggc
     9781 gacactattc gtaaggtcat cccttcaagt tcgatagtct gaatatctac ccgcgtttct
     9841 gtctcacccg caaggacagc gctggcgata tagccctccc tgcgctgcgc aatactttgt
     9901 tgacgcgatg aggggcgacc cgcagcgata aacttttcga ctaactctgc aattcctttt
     9961 tccagtgcca tagtagaact tattttgctg tatgaatgta cagtcttata cacccgtttt
    10021 tgctggatgg atacagggaa attcacgggc gtgaaaaatc tggaaagcgc ctcgcaaatc
    10081 taaagacaaa cccggcagag acgtggtttt gctggagcac tatcagtaag ctagtggttt
    10141 caagggtacg tcaatgacgt acaaaaagga ttgttactct aagccaatgg cgtataatta
    10201 tgctcttcat cgaaacggaa atttttactg aagatgttca aaaactgctg accgacgatg
    10261 aattcagtcg ttttcagttt ttcctggcct tgaatcctga ctatggtgag gtaatacctg
    10321 aaactggtgg gttaagaaag gttcgatggg tgtcaggagg aaaggggaaa cgtgctggtg
    10381 tcagggttat ttactttcat caggttaaac attatgagat cagattactg cttatttatc
    10441 gtaagggtat taaagatgat ctgtctcccc aggaaaaagc aatgctccgg ttgttaaata
    10501 cgaggtggta aatggataag gcactttttg agcggttgac tcacagcatg gcccagatga
    10561 atgaaattat tgaaggaacg cgacagcctt cgcggacttt tgaagttgat gctatgaaaa
    10621 tcaaagaaat acgccgcgct tcagggttgt cacaatctaa atttgccgat cttatttctg
    10681 taagcgtaga cacgttgcgt aactgggagc aggggcgacg ttcaccgacc gggcctgcaa
    10741 aggctttgct gcgagcgatt gccaatgatc cgcaacatgt gctgcaagca cttaaccgtt
    10801 agaggcatgg ttatgccata cagaaagcac tagcattcag aactcctgtg aaagcggcat
    10861 agtggtcgcc tgcccaggag ttttgctatg acccgtaatc gtgctgaacg tcgccaccct
    10921 atgatgcgat taaaaaaact catcgtcgcg ctgatgattg acgtcaatgc tatcggatga
    10981 taccgggggg ttccggaagg aattccttgt ttcccgaact gttaccaaca aaattataac
    11041 gatgaacaag taaagaccgg ttggtggcag tactgaccgg tctgtgatgg atgattaatt
    11101 tcaccttaaa tccgggtgct gttaggatgc tgacattttt cgggtacacc attcagatta
    11161 tgcaaataaa gatccaatca aaaaataaac atccctgaat ataatatatt catagcatcc
    11221 ataactcatc ctgggcgata actaaaccat ttttgagtaa taaatgaaac aactgcaaca
    11281 ctcttattat ttctggaaaa cgcctcgcaa aaacaaaaca acatgcaaaa atccgctttt
    11341 tttaaatgat tttccggcgt aattttcgca catacattaa tgagtaatat aggtaaggtg
    11401 gtggtaatgg cgatgaatac ggtttttctt catttatcag aagaggcaat taaacggctg
    11461 aacaaacttc ggggctggcg taaggtttca cgatctgcaa ttttacgcga agcggttgag
    11521 caatatctgg agcggcagca atttccggtg cgtaaagcaa agggaggcag gcaaaagggg
    11581 gaggtagttg gtgttgatga tcagtgtaag gagcataagg aatgaaaaaa atttttcatt
    11641 cttgaatata aaaaaacaga tgcccttatt ctggtattaa tacaaggctg ttttacttga
    11701 acttataata actgcaactg ttacatcgta tctggaaaac gcctcgcaaa aataaaaaat
    11761 gatgcgtaaa tgagctttct ttaaagattc ggcaagggta cttttctctc catagattca
    11821 aaaatgatat tggcgagata tttatgaact ctctggcagg tatagatatg ggcagaattt
    11881 tactcaattt atcgaatgag gtgattaagc aacttgatga tctcgaggtg cagcgtaatc
    11941 ttcctcgtgc ggacctatta agggaagcgg tagatcaata cctgataaat caatcgcaaa
    12001 cagcaagaac cagtgttcct ggcatctggc aagggtgtga ggaagatggt gtcgaatatc
    12061 agcgtaagct gcgcgaggaa tggtaatggg aaaaaatgat gtcaatcaga ttgctgacaa
    12121 cgtgcgcgtt gttcatgccg ga
//
LOCUS       DAZGJW010000135        11805 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZGJW010000135 DAZGJW010000000
VERSION     DAZGJW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.135"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..928)
                     /gene="yeeY"
                     /locus_tag="ACHKIK_003899"
     CDS             complement(<1..928)
                     /gene="yeeY"
                     /locus_tag="ACHKIK_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9084979.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(974..1798)
                     /gene="yeeZ"
                     /locus_tag="ACHKIK_003900"
     CDS             complement(974..1798)
                     /gene="yeeZ"
                     /locus_tag="ACHKIK_003900"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9084980.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(1881..2135)
                     /gene="yoeB"
                     /locus_tag="ACHKIK_003901"
     CDS             complement(1881..2135)
                     /gene="yoeB"
                     /locus_tag="ACHKIK_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO9084981.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(2132..2383)
                     /gene="yefM"
                     /locus_tag="ACHKIK_003902"
     CDS             complement(2132..2383)
                     /gene="yefM"
                     /locus_tag="ACHKIK_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO9084982.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            2666..2713
                     /gene="hisL"
                     /locus_tag="ACHKIK_003903"
     CDS             2666..2713
                     /gene="hisL"
                     /locus_tag="ACHKIK_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO9084983.1"
                     /translation="MTRVQFKHHHHHHPD"
     misc_feature    2689..2812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            2859..3758
                     /gene="hisG"
                     /locus_tag="ACHKIK_003904"
     CDS             2859..3758
                     /gene="hisG"
                     /locus_tag="ACHKIK_003904"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO9084984.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            3764..5068
                     /gene="hisD"
                     /locus_tag="ACHKIK_003905"
     CDS             3764..5068
                     /gene="hisD"
                     /locus_tag="ACHKIK_003905"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO9084985.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRQDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            5065..6135
                     /gene="hisC"
                     /locus_tag="ACHKIK_003906"
     CDS             5065..6135
                     /gene="hisC"
                     /locus_tag="ACHKIK_003906"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO9084986.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPAAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            6135..7202
                     /gene="hisB"
                     /locus_tag="ACHKIK_003907"
     CDS             6135..7202
                     /gene="hisB"
                     /locus_tag="ACHKIK_003907"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO9084987.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGDSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            7202..7792
                     /gene="hisH"
                     /locus_tag="ACHKIK_003908"
     CDS             7202..7792
                     /gene="hisH"
                     /locus_tag="ACHKIK_003908"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO9084988.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFALPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            7792..8529
                     /gene="hisA"
                     /locus_tag="ACHKIK_003909"
     CDS             7792..8529
                     /gene="hisA"
                     /locus_tag="ACHKIK_003909"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO9084989.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     LLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVESY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            8511..9287
                     /gene="hisF"
                     /locus_tag="ACHKIK_003910"
     CDS             8511..9287
                     /gene="hisF"
                     /locus_tag="ACHKIK_003910"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO9084990.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCRVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            9281..9892
                     /gene="hisIE"
                     /locus_tag="ACHKIK_003911"
     CDS             9281..9892
                     /gene="hisIE"
                     /locus_tag="ACHKIK_003911"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO9084991.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(9989..10966)
                     /gene="wzzB"
                     /locus_tag="ACHKIK_003912"
     CDS             complement(9989..10966)
                     /gene="wzzB"
                     /locus_tag="ACHKIK_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO9084992.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELGKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(11113..>11805)
                     /locus_tag="ACHKIK_003913"
     CDS             complement(11113..>11805)
                     /locus_tag="ACHKIK_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFO9084993.1"
                     /translation="VIGERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANT
                     YLAMRVAYFNELDSYAESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQ
                     LLANYQSVPNNLISAIVDANRTRKDFIADAILSRKPHVVGIYRLIMKSGSDNFRASSI
                     QGIMKRIKAKGVEVIIYEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVA
                     DKVYTRDLFGSD"
BASE COUNT         2892 a         3087 c         3049 g         2777 t
ORIGIN      
        1 aaatggctga ttttccgtca tcagattttg cataaacacc ggcgatcgca ctatttgcta
       61 aaatttcgtc ccgccaccaa ccactggcaa gccccgcggt ctgttcattc cagccaatcc
      121 agacgggttc aaagagggtt tgggcgacca ctttcttctc gattaacgca ccactatcga
      181 gaaaacgttg cgccagataa cggggtaaat agccgcagcc gaggccgcta atttgcagct
      241 ccagcttggt tttaaaatcg aaaacggtaa tagcttcttg ttcgtcgaga agttgtgagg
      301 ccgtagaagc gccggcctgt gcagtgtccc ccaccacaat cgcccgatat cgtttaatga
      361 tacgacggtt taacggttct tcttccaggg ctaacggatg atgaggcgcg accgcaaaga
      421 cttgttccag atcgcccaac cgagagaaac caaactcact ggaagatggt ggctcatgca
      481 tcgcgccaac aataatatcc gctcgcccct gagtcagggc atcccatgaa ccaccgagta
      541 caccattgat aaacttcagg cgcgtcacgc tatgatgttg ataaaaagcc tcaatgagtg
      601 gcgcaagcag agaaaaaggg aaggtgtcgt ccacgccaat caccagctcg ttttcccagc
      661 cttcatgcag tttgatcgcc tgcttttcca gttctcgcac ggtatgcaga acttcccgcc
      721 ctttctctaa taacattttt ccggtgcggg tgaatttagc gcggtgtccg ctgcgatcaa
      781 gcaattgaat attaaggtcg ctttccagct tgtgaacggt gtaactcaga gcggacggcg
      841 tcttgtagag tttggccgac gccgccgcaa aactgccttc tttttctaag gcatcgagga
      901 tcatcaacac atccagcagt ggtttcatac acgccccctt gcagtgtatg cggcgtcctg
      961 ctggcgtgat cgtttactcc agcggcatta ccagcggatc gggatactgg tattcaaatc
     1021 ccagttcatt acaaatccga ctgccatcaa taatcttacc tttgccgctg tccagactat
     1081 ttctgaactg cggaggttcc agccccaata aacgggccat ctgcggatag aaaacattac
     1141 gcgcagggtg agctggcgca catatattat agatgtgtcc gcctttaggt gcctgtaaca
     1201 acagagtgat agcgccaata acatcttcta aatggactaa attaacacca tgttcaccat
     1261 caggggcggt ttttccggca aagaagcgtc cgggatgacg tcccggtccg accaggcccg
     1321 caagacgcag aatatcgacc gaagtaccgg gtaaattgtg cagccagtct tcgagttctt
     1381 ctaatactcg tccactgttg gtcaccggat tacgcggggt ggtttctttc accgtgcctt
     1441 gcgcgtcgcc atagacagat gtcgagctgg taaaaataat gcggggaata cgatgggcca
     1501 gcgcgctatc cactaactct tgtaccgctt gtaaatagaa ctcatcgccg gggccgctac
     1561 gacgtgccgg aagcgtaatg accagcgcat cggcatccat cagggcatcc agatcgtcag
     1621 aatcgcaaac taactcaggc tccatgcgaa gcagatagct atcaatgcca ctcattcggg
     1681 ccgcttcgac accatcttgt gtggttttac tcccggtgac ttgccagcct cgcgctgaaa
     1741 gtgacatcgc cagcggcatg cctaaccacc ctaacccgac aattgcgacc tttttcatcc
     1801 gttatctcct gatacacctt agatctataa ggctacgcta gcgtatcaaa actgacaatt
     1861 cattctatga atgaatctgt tcaataatga taacgacatg ctgcaatgag cagtgaatca
     1921 tcagtaaccg cgtataccag acggtgctcc tctgtaatgc gtcgggacca gaaacctgac
     1981 aaattatgtt tcaggggttc tggtttcccc ttaccttcaa atggtgttct gcgggtatct
     2041 ttgataagtt cattgatctt tttaacaatt cgcttatctg tttcctgcca gtacagataa
     2101 tcatcccatg attcctcaga ccagattagt ttcactcaat aatgtccttt tccgttcctt
     2161 tgcctgattt caggctatcg attgagtcca tcaatctccg ggcgttagcg ggggaacgca
     2221 gtagataagc cgtctcttcc agcgagttgt attcttcgag tgacatcaga acacaagcct
     2281 ctccattctg acgagtaatg aggatcgggg catgatcttc aacggctttc atcattgttg
     2341 ccgacaaatt ctgacgcgct tcgctgtagc taattgtacg catgtaaatc tcctcttttg
     2401 tacagttcat tgtacaatga tgggtgttaa ttcactattt attaattagt ttgtagatca
     2461 agacattgcc agcgagacga aaaatcagac tttgctcttt ttggtgctgt caagttagag
     2521 gacagtcctc ttagccccct ccttttcccg ctcattcatt aaacaaatcc atcgccataa
     2581 aatatataaa aaagcccttg ctttctaacg tgaaagtggt ttaggttaaa agacatcagt
     2641 tgaataaaca ttcacagaga cttttatgac acgcgttcaa tttaaacacc atcatcacca
     2701 tcatcctgac tagtctttca ggcgatgtgt gctggaagac attcagatct tccagtggtg
     2761 catgaacgca tgagaaagcc cccggaagat caccttccgg gggctttttt attgcgcggt
     2821 tgttaacggt tcagacaggt ttaaagagga ataagaaaat gacagacaac actcgtttac
     2881 gcatagctat gcagaaatcc ggccgtttaa gtgatgactc acgcgaatta ctggcgcgct
     2941 gtggcattaa aatcaatctt cacacccagc gcctgatcgc gatggcagaa aacatgccga
     3001 ttgatattct gcgcgtgcgt gacgatgaca ttcccggtct ggtaatggac ggcgtggtag
     3061 accttgggat tatcggcgaa aacgtgctgg aagaagagct gcttaaccgc cgcgcccagg
     3121 gtgaagatcc gcgctacttt accttgcgtc gtctggattt cggcggctgc cgcctttcgc
     3181 tggcaacgcc ggttgatgaa gcctgggacg gtccgctctc cttaaatggt aaacgtatcg
     3241 ccacctctta tcctcacctg ctcaagcgtt atctcgacca gaaaggtatc tcttttaaat
     3301 cctgcttact gaacggctct gtcgaagttg cgccgcgtgc cggactggcg gatgcgattt
     3361 gcgatctggt ttccaccggt gccacgctgg aagctaacgg cctgcgcgaa gtcgaagtta
     3421 tctaccgctc gaaagcctgc ctgatccagc gcgatggcga aatggaagaa tccaaacagc
     3481 aactgatcga caaactgctg acccgtattc agggtgtgat ccaggcgcgc gaatcaaaat
     3541 acatcatgat gcacgcaccg accgaacgtc tggatgaagt catcgccctg ctgccaggtg
     3601 ccgaacgccc aaccattctg ccgctggcgg gtgaccaaca acgcgtagcg atgcacatgg
     3661 tcagtagcga aaccctgttc tgggaaacca tggaaaaact gaaagcgctg ggtgccagtt
     3721 caatcctggt cctgccgatt gagaagatga tggagtgatc gccatgagct ttaacacaat
     3781 cattgactgg aatagctgta ctgcggagca acaacgccag ctgttaatgc gcccggcgat
     3841 ctccgcttct gaaagcatta cccgcaccgt taacgatatt ctcgataacg tgaaagcacg
     3901 cggcgatgac gccctgcggg aatacagcgc gaagtttgat aaaaccacgg ttaccgcgct
     3961 gaaggtgtct gctgaggaga tcgccgccgc cagcgaacgc cttagcgacg agctaaaaca
     4021 ggcgatggcg gtggcagtaa aaaatattga aaccttccac actgcgcaaa aactgccgcc
     4081 ggtagatgtt gaaacacagc caggcgtgcg ttgccagcaa gtcacgcgcc cggtagcttc
     4141 agttgggttg tatattcctg gcggctccgc cccactcttc tcaacggtat taatgctggc
     4201 aactcctgcg cgtattgcgg gctgtaaaaa agtggtgttg tgctcaccgc cgccgattgc
     4261 cgatgagatc ctttatgcgg cgcagctgtg cggtgtgcag gacgtgttta acgtcggcgg
     4321 cgcacaggcc attgccgctc tggcgtttgg tacggaatct gtgccgaaag tggacaaaat
     4381 cttcgggccg ggtaacgcct ttgtcaccga agcgaaacgt caggtgagcc agcgtcagga
     4441 cggtgcggcg atcgatatgc ccgcaggccc gtcggaagtg ctggtgattg ctgacagcgg
     4501 cgctacgcca gatttcgtgg cttctgattt gctttctcag gctgaacacg gcccggactc
     4561 acaggtgatt ttactgacgc ccgctgctga tatggcgcgt cgcgttgccg aggccgtcga
     4621 acgccagttg gcagaactgc cgcgtgccga aaccgcccgc caggcactga acgccagccg
     4681 cctaatcgtg accaacgatt tagcacagtg cgtggagatc tccaaccagt acggcccgga
     4741 gcacctgatc attcagaccc gcaacgctcg cgatctggtc gatagcatca ccagcgccgg
     4801 ttcggtattt cttggtgact ggtcaccgga atcggcaggt gattacgcct ccggcaccaa
     4861 ccacgttctg ccgacttacg gttacaccgc cacctgttcc agcctcgggc tggcagattt
     4921 ccagaagcgc atgaccgtgc aggaactgtc gaaagaaggc ttttctgcgc tggcttcaac
     4981 catagaaaca ctggccgccg ccgagcgcct gaccgcccat aaaaatgccg ttactttgcg
     5041 tgttaacgcc cttaaggagc aagcatgagc accgtgacta ttaccgattt agcgcgtgaa
     5101 aacgtccgca acctgacgcc gtatcagtcg gcgcgtcgtc tgggcggtaa cggcgacgtc
     5161 tggctgaacg ccaacgaata tcccgcagcc gtggagtttc agcttactca acaaacgctc
     5221 aaccgctacc cggaatgcca gccgaaagcg gtgattgaaa attacgcgca atatgcaggc
     5281 gtaaaaccgg agcaggtgct ggtcagccgt ggcgcggacg aaggtattga actgctgatt
     5341 cgcgcttttt gcgaaccggg taaagacgcc atcctctact gcccgccaac gtacggcatg
     5401 tacagcgtca gcgccgaaac gattggcgtc gagtgtcgca cagtgccgac gcaggacaac
     5461 tggcaactgg acttacaggg catttccgac aagctggacg gcgtaaaagt ggtctatgtt
     5521 tgcagcccca acaacccgac cgggcaactg atcaatccgc aggattttcg cactctgctg
     5581 gagctaaccc gcggaaaagc cattgtggtt gccgatgaag cctatataga gttttgtcca
     5641 caggcatcgc tggctggctg gctggcggaa tatccgcacc tggctatttt gcgcacacta
     5701 tcgaaagctt ttgctctggc ggggcttcgt tgcggattta cgctggcaaa cgaagaagtc
     5761 atcaacctgc tgatgaaagt gatcgccccc tacccgctct cgacgccggt tgccgacatt
     5821 gcggcccagg cgttaagccc gcaggggatc gtcgccatgc gcgaacgggt ggcgcaaatt
     5881 attgcagaac gcgaatacct gattgccgca ctgaaagaga tcccctgcgt ggagcaggtt
     5941 tttgactctg aaaccaacta cattctggcg cgctttaaag cctccagtgc agtgtttaaa
     6001 tctttgtggg atcagggcat tatcttacgt gatcagaata aacaaccctc tttaagcggc
     6061 tgcctgcgaa ttaccgtcgg aacccgtgaa gaaagccagc gcgtcattga cgccttacgt
     6121 gcggagcaag tttaatgagt cagaagtatc tttttatcga tcgcgatgga accctgatta
     6181 gcgaaccgcc gagtgatttt caggtggacc gttttgacaa actcgccttt gaaccgggcg
     6241 tgatcccgga gctgctgaag ttgcaaaaag cgggctacaa actggtgatg atcactaatc
     6301 aggatggtct gggaacacaa agtttcccgc aggcggattt cgatggcccg cacaacctga
     6361 tgatgcagat cttcacctcg caaggcgtac agtttgatga agtgctgatt tgtccgcacc
     6421 tgcccgccga tgagtgcgac tgccgtaagc cgaaagtaaa actggtggag cgttatctcg
     6481 ctgagcaagc gatggatcgt gccaatagtt atgtgattgg cgatcgcgcg accgacattc
     6541 aactggcgga aaacatgggt attaatggtt tacgctacga ccgcgaaacc ctgaactggc
     6601 caatgattgg cgagcaactc actaaacgag accgttacgc ccatgtagtg cgtaacacca
     6661 aagagacgca aattgacgtc caggtgtggc tggatcgcga aggtgacagc aagattaaca
     6721 ccggcgttgg cttctttgat cacatgctgg atcagatcgc cacccacggc ggtttccgta
     6781 tggaaatcaa cgtcaaaggc gacctctata tcgacgatca ccacacagtc gaagataccg
     6841 gcctggcgct gggtgaagcg ttaaaaattg cccttggcga caaacgcggt atttgccgct
     6901 ttggttttgt gctaccgatg gacgaatgcc ttgcccgctg cgcgctggat atctctggtc
     6961 gcccgcacct ggaatataaa gccgagttta cctaccagcg cgtgggcgat ctcagcaccg
     7021 aaatgatcga gcacttcttc cgttcgctct cttacaccat gggcgtgacg ctacacctga
     7081 aaaccaaagg taaaaacgat catcaccgtg tagagagcct gttcaaagcc tttggtcgca
     7141 ccctgcgcca ggccatccgc gtggaaggcg acaccctgcc ctcgtcaaaa ggagtgctgt
     7201 aatgaacgtg gtgatccttg ataccggctg cgccaatctg aactcggtga agtctgccat
     7261 tgcgcgccac ggttatgaac ccaaagtcag ccgtgacccg gacgtcgtgt tgctggccga
     7321 taaactgttt ttacccggcg taggcaccgc acaagcagcg atggatcagg tgcgtgagcg
     7381 cgaactgttt gatctcatca aagcctgtac ccaaccggtg ctgggaatct gcttagggat
     7441 gcaactgctg gggcggcgca gcgaagagag caacggcgtc gacttgctgg gcatcattga
     7501 cgaagacgtg ccgaaaatga ccgactttgc tctgccactg ccacatatgg gctggaaccg
     7561 cgtttatccg caggcaggca accgcctgtt tcagggaatt gaagacggcg cgtactttta
     7621 ctttgttcac agttacgcaa tgccggtcaa tccgtggacc atcgcccagt gtaattacgg
     7681 cgaaccgttc accgcggcgg tacaaaaaga taacttctac ggcgtgcagt tccacccgga
     7741 gcgttctggt gccgctggcg ctaagttgct gaaaaacttc ctggagatgt gatgattatt
     7801 ccggcattag atttaatcga cggcactgtg gtgcgtctcc atcagggcga ttacggcaaa
     7861 cagcgcgatt atggtaacga cccgctgccg cgattgcagg attacgccgc gcagggtgca
     7921 gaattgttgc atctggtgga tctgaccggg gcaaaagatc cggctaaacg tcaaatcccg
     7981 ctgattaaaa ccctggtggc gggcgttaac gtgccggtgc aggttggcgg tggcgtgcgt
     8041 agcgaagaag acgtggcggc gttactggaa gcaggcgttg cgcgcgtggt ggttggctcc
     8101 accgcggtga aatcaccaga gatggtgaaa ggctggtttg aacgtttcgg tgccgatgcc
     8161 ttagtgctgg cgctggatgt ccgtattgac gagcaaggca acaagcaggt ggcagtcagc
     8221 ggctggcaag agaactcggg cgtttcactg gaacaactgg tggaaagcta tctgcccgtc
     8281 ggcctgaaac atgtgctgtg taccgatatc tcgcgcgacg gcacgctggc aggttctaac
     8341 gtctctttat atgaagaagt gtgcgccaga tatccgcagg tggcgtttca gtcctccggc
     8401 ggtattggcg acattgatga tgtggcggcc ctgcgtggta ctggcgtgcg cggcgtaata
     8461 gttggtcgtg cattactgga aggtaaattc accgtgaagg aggccatcgc atgctggcaa
     8521 aacgcataat accgtgtctc gacgtccgtg atggtcaggt ggtgaaaggc gtacagtttc
     8581 gcaaccatga aatcattggc gatatcgtgc cgctggcaaa acgctacgct gaagaaggtg
     8641 cagacgaact ggtgttctac gatatcaccg cttccagcga tggccgtgtg gtagataaaa
     8701 gctgggtatc tcgcgtggcg gaagtgatcg acattccgtt ctgcgtggcg ggtgggatta
     8761 agtctctgga agatgccgcg aaaattcttt cctttggcgc ggataaaatt tccatcaact
     8821 cccctgcgct ggcagaccca acattaatta ctcgcctggc ggatcgtttt ggcgtgcagt
     8881 gtattgtggt gggtattgat acctggtacg acgctgaaac cggtaaatat catgtgaatc
     8941 aatataccgg cgatgaaagc cgcacccgcg tcactcagtg ggaaacactc gactgggtac
     9001 aggaagtgca aaaacgcggt gccggagaaa tcgtcctcaa catgatgaat caggacggcg
     9061 tgcgtaacgg ttacgacctc gagcagctga aaaaagtgcg agaagtttgc cgcgtcccgc
     9121 tgattgcctc cggtggcgcg ggcaccatgg aacacttcct cgaagccttc cgtgatgccg
     9181 acgttgacgg cgcgctggcg gcttccgtat tccacaaaca aataatcaat attggtgaat
     9241 taaaagcgta cctggcaaca cagggcgtgg agatcaggat atgttaacag aacaacaacg
     9301 tcgcgaactg gactgggaaa aaaccgacgg actgatgccg gtgattgtgc aacacgcggt
     9361 atctggcgaa gtgctaatgc tgggctatat gaacccggaa gccttagaca aaaccatcga
     9421 aagcggcaaa gtcactttct tctcgcgcac taaacagcga ctgtggacca aaggcgaaac
     9481 gtcgggcaat ttcctcaacg tagtaaatat tgcgccggac tgcgacaacg acacgctact
     9541 ggtactggcg aatcccatcg ggccaacctg ccacaaaggc accagcagct gcttcggcga
     9601 caccgctcac cagtggctgt tcctgtatca actggaacaa ttgctcgccg aacgcaaatc
     9661 tgccgatccg gaaacctctt acacagccaa actgtatgcc agcggcacca aacgcattgc
     9721 acagaaagtg ggcgaagagg gcgtggaaac cgcactagca gcaacggtac atgaccgctt
     9781 tgaactcacc aacgaggcgt ctgacttgat gtatcacctg ttggtgttgt tgcaggatca
     9841 ggatctggat ttaacaacgg tgattgagaa cctgcgtaaa cggcatcagt gagttgcggg
     9901 gtaagcggat gcgatgttgt tgtcgcatcc ggcaaaaaaa acgggcaagg tgtcaccacc
     9961 ctgcccgttt tctttaaaac caaaaagatt acttcgcgtt gtaattacgc agagcgttac
    10021 gccccagcac aatccccgcg ccaaccatgc cgcccagcag caccgccaga atcaaagtga
    10081 ttgccttttt cgggctatcg cgacgaatag gtaacgtcgg tttcatcaca tagcggtaag
    10141 catgaatatc aagatcatca acttttaagc tttcgatatc cagcaggttt tgacgtgtct
    10201 gatagtagtt tggtgagaac accaacggac gagtcgcttc atgcttaatc atcgactcca
    10261 gcgcttcgct ccccaaaagg aacaacgtat cttgtgtcac atcttcagtc tgctgaacct
    10321 gtggctttgt cacctgcgcc tgattcgcat actgcaacgc ttcctgaatc tgacggatac
    10381 gcagatcttt ctgctcctgc gcaactactt cctgagtttt cagagaatcc tgcaagtttt
    10441 tcatacgcag cgcgatgttg tctttcaggt cttttcccag ttcttcattt acctggtcgt
    10501 caacttgctg gatatacttc gccagctgct tctgagcacc ttcaggtgtt tcaccaacat
    10561 aagacaccgc taaaggtaat gactgatttt tgacggtagg ctcaatggtc agcttttctg
    10621 cttcttcctg gttatccagc gtttccgcta atgctgagaa tgcagtgctg taacgtccga
    10681 taagtgaagc ctgaatgtcg gagactttcg gtacagccga tccgtagatc acattcatcg
    10741 cgttggtata accagcgatt tgccccacat caggttgagt aacgatagct gtggaagtcc
    10801 acttttcttt ggcaacggcc agatacccta tagccagggc aatagcaaca atgacagaga
    10861 tgataattgt catcttgcca cgccacaact gcaccagtaa atcaatcaaa tcaatctgtt
    10921 ccgggtcatg gttttgccca gaaacattat tattttctac tctcatcatt accctaacgg
    10981 acgaaataat tttaagtcgc catagtttag cgataatcgc catgaatgct ataggataaa
    11041 tgataaaaat aatgaattat tacaaaggga acataagaga atcagggcga aaatcgccct
    11101 gataacagga tgttagtcac tgccaaagag atcgcgggtg tagactttgt ctgccacatc
    11161 cttaagctct tctgccatac ggttggagat aattacgtcg gcttgttgtt tgaaagtggc
    11221 gagatcacgt tccagccgag agttgaagaa tgagtcttct ttcatcactg gctcgtagat
    11281 gattacttca acacctttcg ccttgatacg cttcataatc ccctgaatag aagacgcacg
    11341 gaagttatct gaaccgctct tcataatcag gcgataaata cccaccacat gcggtttgcg
    11401 tgacaaaatg gcatcggcaa taaaatcttt acgcgtgcgg tttgcatcga caattgccga
    11461 gatcaggtta ttcggcacag actgatagtt cgccagtaac tgcttggtat ctttcggcag
    11521 acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag
    11581 acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcgt agctatccag
    11641 ttcattaaag tacgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc
    11701 tgcttcggtt gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa
    11761 cagagcagca aaacgttctg cgcgttctga acgctcaccg atgac
//
LOCUS       DAZGJW010000136        11800 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZGJW010000136 DAZGJW010000000
VERSION     DAZGJW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.136"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..214
                     /locus_tag="ACHKIK_003914"
     CDS             <1..214
                     /locus_tag="ACHKIK_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084994.1"
                     /translation="SLKQEFSGVPDDAADALSSMPELIRAAFFLLSTREYKSTGLDVL
                     NIAADYAEYVAEARYRRKFPEDVSHA"
     gene            207..440
                     /locus_tag="ACHKIK_003915"
     CDS             207..440
                     /locus_tag="ACHKIK_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084995.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLDPVIMELQEIDILHDRYKEQHTGE"
     gene            446..745
                     /locus_tag="ACHKIK_003916"
     CDS             446..745
                     /locus_tag="ACHKIK_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9084996.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVTIKGVEERRITY
                     RREGYEYDCVMPVYQFRRDFSLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            742..2142
                     /locus_tag="ACHKIK_003917"
     CDS             742..2142
                     /locus_tag="ACHKIK_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO9084997.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            2343..2594
                     /locus_tag="ACHKIK_003918"
     CDS             2343..2594
                     /locus_tag="ACHKIK_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084998.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            2591..2896
                     /locus_tag="ACHKIK_003919"
     CDS             2591..2896
                     /locus_tag="ACHKIK_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9084999.1"
                     /translation="MSEPKFGEKLYKHNGLITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            2906..3316
                     /locus_tag="ACHKIK_003920"
     CDS             2906..3316
                     /locus_tag="ACHKIK_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO9085000.1"
                     /translation="MTAQIAAYGRLVDDPLVKQSSKGTPMTLARMAVSLPCSQAHDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGYQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGDDDPYGDDIPF"
     gene            3327..3599
                     /locus_tag="ACHKIK_003921"
     CDS             3327..3599
                     /locus_tag="ACHKIK_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO9085001.1"
                     /translation="MVHDRIAEELEAKGFYRRASARWGEVMLLVETDKERHQVTMRRL
                     ECSRKAQRPPEPPDNYGDLKKAVDRTYAEMGMDGAGDEIWRNYQDS"
     gene            3556..3684
                     /locus_tag="ACHKIK_003922"
     CDS             3556..3684
                     /locus_tag="ACHKIK_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO9085002.1"
                     /translation="MVLVMKYGEITRTANHTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            3725..3949
                     /locus_tag="ACHKIK_003923"
     CDS             3725..3949
                     /locus_tag="ACHKIK_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085003.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKGIRD"
     gene            4241..5398
                     /locus_tag="ACHKIK_003924"
     CDS             4241..5398
                     /locus_tag="ACHKIK_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO9085004.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGATFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
     gene            5438..6010
                     /locus_tag="ACHKIK_003925"
     CDS             5438..6010
                     /locus_tag="ACHKIK_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO9085005.1"
                     /translation="MGEKIMKSMEIRSSEITTSGSNTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            6012..7223
                     /locus_tag="ACHKIK_003926"
     CDS             6012..7223
                     /locus_tag="ACHKIK_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9085006.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLNGNAYAVIQWGRDGFPAALHPYPPQSVNMEQTGEHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            7220..7558
                     /locus_tag="ACHKIK_003927"
     CDS             7220..7558
                     /locus_tag="ACHKIK_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO9085007.1"
                     /translation="MNIGRLRDRVTIQTLKQTRAMTGEILETWLDGHTLWASVNMISS
                     KEAISSGAELATGTVRIWIRYRKDINATSRIKVNTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            7555..7848
                     /locus_tag="ACHKIK_003928"
     CDS             7555..7848
                     /locus_tag="ACHKIK_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO9085008.1"
                     /translation="MTELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRRF
                     DDGLEFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            7848..8288
                     /locus_tag="ACHKIK_003929"
     CDS             7848..8288
                     /locus_tag="ACHKIK_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO9085009.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPIDGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREDEERAANRNNWMYDADE"
     gene            8272..8454
                     /locus_tag="ACHKIK_003930"
     CDS             8272..8454
                     /locus_tag="ACHKIK_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085010.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            8577..8933
                     /locus_tag="ACHKIK_003931"
     CDS             8577..8933
                     /locus_tag="ACHKIK_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO9085011.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            8917..10578
                     /locus_tag="ACHKIK_003932"
     CDS             8917..10578
                     /locus_tag="ACHKIK_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO9085012.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLVSRHYLPEFQLQNPANKNRA
                     IYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            10592..10873
                     /locus_tag="ACHKIK_003933"
     CDS             10592..10873
                     /locus_tag="ACHKIK_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085013.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRALELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            complement(11632..11775)
                     /locus_tag="ACHKIK_003934"
                     /pseudo
     CDS             complement(11632..11775)
                     /locus_tag="ACHKIK_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pheromone autoinducer 2 transporter"
BASE COUNT         3203 a         2778 c         3318 g         2501 t
ORIGIN      
        1 ttccctgaaa caggaatttt ccggcgttcc tgatgatgcg gctgatgcgc tttcgtcaat
       61 gccagaactt attagagcgg cttttttctt actttccacg agagaatata aatcaacggg
      121 gcttgatgta ctgaatatcg ccgccgatta tgcggaatat gtggcagagg cgcgttatag
      181 aagaaaattt cctgaggatg taagccatgc gtgatattta ccaccaaaca atagaccgcg
      241 catttcttgc actttctcac agtgaaaaca tgctggaaat attgcgcata tggcttgaaa
      301 cacttggcga caatgaacgc gacaaacaaa aatcaagaat tgccacggca ttaataacgc
      361 ttcttgatcc tgtaataatg gaactgcaag aaatagatat attgcacgac agatataaag
      421 aacagcacac cggagaataa aaataatgaa acttaaatat tctggcttaa ctgccagtgg
      481 caacactcac cctaaattta cgcgcggcga tatttaccgc gaccagtacg gcggcaccgt
      541 aacgattaaa ggtgtagaag agcgacgcat tacctaccgc cgtgaaggct acgaatatga
      601 ctgcgtgatg cctgtttatc agttccggcg tgatttttct ctggtacagg cagcgccccg
      661 cagtaaacca accagcaggg agaaagcacg cgccaatatt caggaaataa aaaagatgct
      721 taacgtattc aggggcaaga aatgaaaagc gcaccgaact taaaaaaaca gccttacgac
      781 aagatgaccg aagtcatcat ttttgcgggt agtgatgcgt gggcacatgc gaaacagtgg
      841 caggaacagg acgggcgact ggctggcgat aatgtgcctc ccgttgtgct ggctgatgat
      901 caactggatg aactggcaga cctgagaatc atcgacgagg ggcgctattg tgtccggctg
      961 tacaaggcag gccacatcag gccatcaaat attaatgcca ttgcgcacaa gctggcggcg
     1021 gcgggtgtaa ctgatgcgaa ttattacccc gaagggatgc acagccatat gcgggagaac
     1081 tggcgcgaat acctggaacg ggtgcgtggg aaagagccgg cggaagaaaa aaaccaccag
     1141 cgaaaaacca cgctaccgat gagcgttgga tctaccggat acgacacgca actggattac
     1201 gtggttaagg ggattattcc ggcggtatcg ctatgcagca tatacggggc tagcgggtcc
     1261 tataaatcat tccttgccgg atcgtgggcg tgccatgttg ccactggtcg ccagtgggga
     1321 ggccgcaggg ttgcgcatgg tgcggttctc tatgtggttg gtgaaggcgg tataggtgtt
     1381 ccgcgtcgtg taaaagcctg ggaggttgtg cacgatgagc aggtgaaaaa tctgtatctg
     1441 gtaaaccgcc ccatctttcc ggctgccccg cttgatgttg atgaaatggt tatcgctgcc
     1501 cgtcaggtgg agcgggaaac gggtaaacct gtacgcatga ttattctgga tacgctggcg
     1561 cgttgctttg gtgggaatga tgaaaatgat tcccgtgata tgggggcgtt tatccgtggt
     1621 tgtgacgaac tgaaacgacg cacaggggcc acggtgctgg tggttcacca ttccggcaag
     1681 gatgagacga aaggcgcgcg cggttccagt gcatttcgtg cttcgctgga tgctgaatac
     1741 cggatacgca gggaggacgc aggaagcgaa gcgctggtta tctcatgcac caaaatgaag
     1801 gacgcggagg aactcaaaga agccgcatat gacttacgcg tggtggagct ttttaccgac
     1861 gctgacggtg aattaatcac gtcgctggtg gtggtggatg atccgcgccc tcctgttgaa
     1921 ctggagcgca tcgaggaggc agggaacaag acggaaaacc ataccgcgct atgggggtgc
     1981 atccgttcac gcacacagaa cggcgacaag tgcacgatcc cgctgttacg tgatgacatg
     2041 aaaaagctgg ggtatgaaat gaaaaacttc cggcgctggc tgtacaagct ggaaaaagat
     2101 ggggttattc gtatcgatgg ggatgatgta gcgccgctat aaaagtgagg agcaaaagcg
     2161 aggtggatag aaagagggcc aaaattagcc tgctctccct cacttttcga cctgtataca
     2221 tcctcaaaag tgaggggtaa aaaaatactt atgaaacaca cacatagaaa aaccgaaaat
     2281 cccagctgcg acgaagtgag acgcttgaaa aagtgaggcg aaaaagtgag aggttgcgag
     2341 aaatgaccca aaaacgcaga gacagaacag agccaaaata taaagcgtta gacatgactg
     2401 agcacacctt aaaggtggca atcagaacga tagaccgcca cacgcgggaa ggatacgcga
     2461 aggaacatcc cgacctgata agcgcattca tgaccacggc ggcggcaaac tttgccacgc
     2521 tgacagaacg ggagattgcc gaagcggaac aggtaacaac catcaacgtt aaaaccggag
     2581 aggtggaatc atgagcgaac caaaatttgg tgaaaaactt tacaagcaca acggactgat
     2641 cacgattctg caaataagcg cagctacacc aggctggtgg gttgaaacag acgaaggatc
     2701 ctcacccgtg gcatcatggg cgctttgtgt gcttagttac cctgaccgcg atgtgtatca
     2761 ggatattctg cctgtgatat ccactgacaa aggcatgaaa cccgtagaca tcaaaaacat
     2821 gggttttcag tgcgtaatgc tcactgaaaa aatgatggaa gagatgaaaa agaacaacgc
     2881 cggatcgctt cactgaggtg gcaatatgac agcacaaatt gcagcttacg ggcgcctggt
     2941 ggacgacccg ctggtaaaac agagcagcaa gggcacaccg atgactctgg cgcgtatggc
     3001 ggtatcgttg ccatgcagcc aggcacatga cggacaggcg acgttatggt tatcggttat
     3061 cgcatttggc aaacaggccg atttcctggc taaacatcaa aaaggcgatg ttgccagcgt
     3121 atccggcacg atgcaggtaa gccagtggac cggacagaac ggggaaacac ggcaggggta
     3181 tcaggttatt gcagacagcg taatcagtgc ccgtgcggca cgtcctggcg ggaacagacg
     3241 caaaaccaca ggcacacagg gtaatcagcc accagcggga gacgatgacc cctacggtga
     3301 tgatattccg ttctgagggg gtgacgatgg tacatgaccg catagcggag gaactcgagg
     3361 caaaaggctt ttaccggagg gcgtcggcgc gatggggtga agtcatgctg ctggtggaga
     3421 cagacaagga acggcatcag gttacgatgc gacggctgga atgttccagg aaggcacaga
     3481 ggccaccgga gccgccggat aattacggag acctgaaaaa ggcggtcgat cgcacttatg
     3541 ccgaaatggg tatggatggt gctggtgatg aaatatggcg aaattaccag gacagctaat
     3601 cacacagccg gagaaatccg gctttttttg caccagttga aacggtatgg cgcattaccg
     3661 ggttttcgtc acggtcaggc atagttacta tctgaaacaa acagacacaa cagaggaaaa
     3721 aacaatgccg atgaaatttg atgagatatt aaaacagcgt gataaatacc atgctgacaa
     3781 catggagacg atgagcatca atgattaccg cgcattcctg gagacgggcg cactgattga
     3841 aaaggatcag catggttttg tgagatgtgc tctgtccggt gaaatgctgg cggtaaatcc
     3901 tgaacagata gatgcattga tagaatttct gaaagggatc agggactgag cacgcataca
     3961 gccggagcaa tccggctttt tgtcattttt tgtaaattat ttgttcgcgg ttgttccacg
     4021 ttgttcactg acaggatcgg catattttac ccgaactgaa tcatgattat tctcgcccgt
     4081 ggtgccagga cgctggggcc acttttccgc ctgttaatgt tctcgccaat attcattacc
     4141 aggcgggaaa acgatcggtg cgattgctga tttccttatg aaaaacggtt gagtttttgc
     4201 cgcgtcctgg agttccttac ttaaccccag gacttttttt atgccgagaa taatcgaatt
     4261 acgccagcag aaaaccgcca ttaaaaatca gatgcgcgac atgctggaga acgcggaaaa
     4321 agaaaaccgc agtcttaacg atgctgaggg cgcaacattt gacgaattac gcgctaaagc
     4381 tgaatccctc gataaagaca tttcccgcct tgaagccatt gcagacgaag agcgcagcaa
     4441 gccaggtaaa agcagtcaga ccactgaccc cgcagaactc cgcaactaca ttctgacagg
     4501 tgaaacccgc gcattaagta caggcgttcc cgctgatggt ggttataccg ttatccctga
     4561 actgaacacc gaaatcatgc gaatgctggc ggatgaatcc accatgcgcc gcatctgtac
     4621 cgtgaagaaa atcagcagca acgagtttaa gcagcttgtt tccgctggcg gtgcgaccgt
     4681 taaccacggt gaagagggta aggcacgcga acagaccagc accccgcaga ttaacgaggt
     4741 gagcattaag ctgtatccgg tctatgcgta cccgcgcacc acacaggaaa tcgtggattt
     4801 ttccgatgtg gacatccttt catggctgac gggtgagatt ggcgacacct tcacggaaac
     4861 cgaagaaagc gatctggttg tgggcgatgg tgacaaaaaa gcaaaaggct ttttatccgt
     4921 accccgtgca gagaagaacg acaaagagcg tgattttggt acgttacagg taattaaacc
     4981 ttccgaatct ctggcgtgga catctgcgga cccgctgatc gacctgaaat ttgcattacg
     5041 taaaaaatac cgcaaaaacg cggtctgggt ggttaactcc acgacggcgg caaaactcca
     5101 gaaggtgaag aacgcgaacg gtgattatat ctggcgcgac cgtttacagg cgggtgatcc
     5161 tgatacgttg ctgggccttc cggtcgaata tctggagttt atgcctgata acgttattgc
     5221 cctgggtgac ttcaaacgcg gttactacat tgttgatcac gaaacaggtg ttcgcaccag
     5281 accggacaac ctcacagagc cgggcttcat caaaattttc acgcagaaat atttaggcgg
     5341 tggcgtggtg gattcgaacg cgatcaagat tctggaactg ccacaggacg acgattaaca
     5401 gcatacagaa ggggcttaaa agccccttta gtgttttatg ggtgaaaaaa ttatgaagag
     5461 tatggaaatc cggtcatcgg aaatcaccac cagcggcagc aacacgctta caggctacgt
     5521 tgttcgctgg gataagcttt cagaactgtt atggggtgag ttttacgaaa aattccagcg
     5581 gggggcgttt actgaatggc ttgcggcggg taatgacgtt cgcggcctgt atgagcatga
     5641 ccacagtatg ttactggggc gcacccgttc cggcacgctg aaactggaag aggatgaaac
     5701 agggttacgc tttgaactga ccccaccgga taccagtaca gggcgcgacg ttatcgaact
     5761 ggttaagcgt ggtgatatat ccggcatgag ctttggcttt cgttcccgta aggatgtatg
     5821 ggataccaca acagatccat gcgtgcgcac cgtgctggtt gcggaactgt acgaaattac
     5881 cgtcacatcc gtaccggctt accctgattc cggcgtggag ctggcccgcc gctccctgta
     5941 cgagcagcac cccgaaaaaa tgccgcgtgc ggataatcgc cgctggtggg cggatttagc
     6001 gggggtgtga tatgtggcct ttcagaagaa aaaaagagca gcgcagcatg acgcttgatg
     6061 agtttatggc gctggctggc acatcgaaca cgggggcggg tgagtacgta tcatcgggga
     6121 cagcggaatc actgcccgcc gtcatgaacg ccgtgacggt catctctgag gcggtggcta
     6181 ccatgccgtg ttacctgtac ctggtacgca atgagaaggg gaaggaggcc cgcgagtggc
     6241 ttgattctca tccggtcgat cacatcctca acgagcgccc gaacgcgtgg cagactccct
     6301 accagtttaa gcgaatgatg atccgccact gcctgttaaa cggtaatgct tacgcggtga
     6361 ttcagtgggg gcgtgatggt tttccggcgg ctttgcatcc ttacccgccg cagtcggtga
     6421 acatggagca gacaggagaa cacaactggc gctactgcat cactgacgct tacaccggaa
     6481 acacccgcaa ctatttaccg tgggaggttc tccaccttcg ttactccacg gatgacggtt
     6541 ttatggggcg ctcacctgta accatctgcc gcgaatcgct ggggcttggg ctggcccaac
     6601 aacgccacgg cgcgagcgtg atgcgtgatg gcatgatggc ggcaggggtt atcacgtcag
     6661 gcgaatggct ggacggcgtg aagggcaaac aggcattagc cgcactggaa cgctacaaag
     6721 gggccagaaa cgccggaaaa acgcccatcc ttgaaggggg tatgagctat cagcagctgg
     6781 gcatgagtaa tcaggatgct gaatggctgg cctcccgtcg cttcaccatt gaagacatcg
     6841 ccagaatgtt caacgtctcg ccgatttttt tgcaggaata cagcaacagc acctacagca
     6901 atttcagcga ggcaagccgc gcatttctca ccatgacgat gcgcccgtgg ctggcgaact
     6961 ttgagcagca gataaaaaac gccctgctgg tggcctcgcc tgtacctggt atccggtatc
     7021 aggtggagtt tgacagcgcg gacctgttac gggccacacc tggcgaacgc tttgccacct
     7081 atgagcgcgg catcaaatcc ggcgttatgt gtccgaacga agcccgcgaa cgtgaaggac
     7141 tgtcaccgcg tgatggtggt gatgagttca gccaggcatg gaaacaggaa gtaaaaatca
     7201 gcgagggaga aaaaccggaa tgaacatagg gcgactgcgt gacagggtaa cgattcagac
     7261 cctgaaacag accagagcca tgacgggtga aatactcgaa acgtggctgg acggtcacac
     7321 actctgggca agcgtgaaca tgatcagcag caaggaggcc atttcatcag gtgcagagct
     7381 ggcgactgga actgtaagga tatggatacg ctaccggaag gacatcaacg ccaccagtcg
     7441 gataaaggtc aatacggggc cgctggcggg gcgtgtactg aatatcatcg ggcaaccgct
     7501 gccagatgcc gccaggacac gccttgaaat tctttgtcgt gagggcgcgg aaaaatgaca
     7561 gaacttatca ccctggaaga agtgaaactc cattgccgca tcgatggcga cgatgaagac
     7621 cagttaatca gcggatacat tgccgcatcg cttgaggcgt gccagataca cataggcagg
     7681 cgctttgatg acgggctgga gttcacgcca gccataaaga ttggctgcat gatgtttatc
     7741 gctcacctgt atgaaaatcg ccagattgtc gcggataacg caaaaacgcg cgtacccatg
     7801 acgataggcg cactctggac ggcttaccgt gatgtggggg tgtactgatg ccgtggcaac
     7861 cattaagacg atgtacagaa ccaggctgta acaggcgcgt taagtccggt aagtgtgagg
     7921 agcacaggcg ggcggcatgg cgtgcagagg atgccagacg aggacaccgc cgcgcgcgtg
     7981 ggtactccag acagtgggac aaataccgcg ccatgtatct gagtaaaaac ccgttatgcg
     8041 tgcgatgcct tgagaagggg atatatacgc ccgccgtggt ggtggatcac attatcccca
     8101 tcgatggcgg cgatgatgtt ctcttctggc ccgaatggaa ccatcagcca ttgtgtcagg
     8161 cgtgtcataa ccagaaaacg aaatggcttg atccggcaac aaaaagcaag cgtgccgcag
     8221 gtggatttcg tgaagatgaa gaacgggccg ctaaccgcaa taactggatg tatgacgctg
     8281 atgaatgagc gggaacaaaa ccgccttatc cgtggactta taaggcagcg tgacgcatgg
     8341 aagacacagg aaacagggca taaagataaa gcgtcaggac gcgcagaacg catcacagcg
     8401 aagcgattaa ccgaccgtga ccgcgaggtt atggaatgtt tccgcaatcg ctgatgaggc
     8461 tgtatggcgg ggtggggggc gttttcagga caaacccgac cccgccgggc accgaacgcc
     8521 tccccaaatt tttatgcacg ggaatttttt gaaaaataat ctgacgaaaa ataagcatgg
     8581 caagaccacc gaaagccccc gcctacctgg atgacatcgc cgtgaagcag tggcgggaaa
     8641 aatcgcggca gcttgcggaa cggggagacc tgacccccgc cgactggagc aatctggaac
     8701 tgtattgcgt caattactcg atataccgca aagccgtcgc agaccttgcg gcgcgcgggt
     8761 tcagcattgt taacagtcag ggcggcgaga gcagaaaccc cgcattaagc gcgaaatccg
     8821 acgctgaaag agtgatgata aaaatggcct ccttgcttgg ttttgacccg ataagccgcc
     8881 gtaaaaatcc accggaaaca gaagaagagg acgagcttga ccgcctggaa taagtacgca
     8941 gaagacgtaa aaacgggcaa aattccggcc tgtaaacggc tgaaacaggc cgttaaacgg
     9001 tacttttcgg atcttaaaag ccccctttac acgttcgatc gtgaggttgt ggagcggttt
     9061 attgcctttt ccagggtgtg cccgcacgta aaaggaccga tgcgtggcag acccattgag
     9121 ctggagccgt ggcagcagtt cgcctttgcg tgcatcctcg gctttaaggt taaggccacc
     9181 ggacggcgca aatacacgag cgcctttatc gaagtgccgc gcaaaaatgc gaaatcaacg
     9241 accgccgcga ttctggctaa ctggtttctg attatggaga acggtcagca ggatatttac
     9301 accgcagcgg tgagccgtga ccaggcgcgg atcgtctttg atgatgcgcg tcagatgtgc
     9361 cttttatccc gaccgttacg caggcgggtg aatattcagg cgcataaggt gatacacccg
     9421 aaaagcaaca gcctgttaaa gccgctggca gcaaaagcgg caaccatcga gggcacaaac
     9481 cccagccttt ccatcgtgga cgaataccac ctacaccccg ataacggcgt ttattctgcg
     9541 cttgagctgg ggatgggcgc acgtcccgaa gggctgttat ttgccattac cacatcaggc
     9601 agtaacgtcg tttcagcctg taagcagcac tatgattatt gctgccagat cctggacggc
     9661 gaagaggtca acgattcaat ttttgtactg atttacgaac tggacgacga aaacgaggtt
     9721 gatgatccgg cgatgtggat aaaagcgaac cctaatattg atgtttccgt ggatcgtgaa
     9781 aaactggcct caaccatcca gaaagcgcgg ggtattccgt cgcagtgggt ggagatgatg
     9841 acaaagcgat ttaatatctg gtgtcagggg actaccccgt ggatgggtaa cggcgcatgg
     9901 gctgagtgtg ccggaacgtt cacggaggaa gatttacacg gccaggagtg ttacgcgggg
     9961 ctggacttat catcaaccag cgatatttcc agcgtgtgct atgcctttcc ggtcggtaaa
    10021 accattatgc tggtttcccg tcactatctg ccggaatttc agctacagaa ccccgccaat
    10081 aaaaaccgcg ccatctatcg tcagtgggct aaagcaggct ggatacgcac aacgcctggc
    10141 gactgcattg attacgaccg gatcagagat gacatcatgc aggacgcgga gaaatttaat
    10201 atcaggctgg tgggctttga tacgtggaac gccacgcatc tcagaacgca attacagggg
    10261 gcaggttttg aggtggagcc gttcccgcaa acctacctca gattcagtcc ggcggcgaaa
    10321 tcgttcgaag tttttgttaa ccgcagggtg attgtgcatc gtggcgatcc ggtgttgtcc
    10381 tggtcgatga gtaacgtcgt gatgcagagt gacgcgaacg ccaatatcaa gccgaacaag
    10441 aaaaaatcac cgaacaagat agacccgagc gtggcggcgc tgatggcgtt tggtacattc
    10501 caggcagagc atgaggattt tgcttttgat ataagcgaca gccaccgcca gaaactggaa
    10561 gaatttagcg gggtgtaatg aggtcagcag catgacagaa gccgaaataa cgggattaat
    10621 ccgccgcgtt gccggaatca gccagcaggc tgacgaacag gccacgcagc cggacagcgt
    10681 gacagccgaa aactatgcgc gtgttgttgc tgaggtgatg cgccgtgatg gtatagaact
    10741 taatggcgtg gatatgcgca acatacgaac cagagctctt gagttgctgg cctaccgtcg
    10801 ccgcgtggag atgtatcggg agaaggagaa aataacgtac cactggaaga agccggagcg
    10861 gttgcggcgg taactggttg atattcccga cggaccaaaa atgggtggct acatccctcg
    10921 cttctgagga ctgttaacaa gctgcctgaa agacagtcac aaactacgaa attttcgcag
    10981 tttaattaat gatcagctta atcgctaacc cgctgatatt ttcggaaacc tcaatttgag
    11041 gaagtcggcg cggtaactcg ctgaacttta agcaaagtgc aaaattgcgc ttgctgaata
    11101 atcattatga ttacgcagat gattaaggaa tgaccgaagg cggaaattcg cctgtggtta
    11161 atgggggagt tgcagatctg caactcgacc atgaaactac ggaaactacc cgtagtttgg
    11221 gtagtaagag taacacccag attttgggtc ttactcgtga tacccaaatt tggggtatca
    11281 gtggcaacca taacgacttt cgttacggtt gatgctttta ccccattggg ggaaaggtat
    11341 tacgataatc ataacaccta ccgaacaggt aaagcccacc agcctgatta acgtttaacc
    11401 ggaaaaaaat tccggtaggt ggggatcccc atatcgacat taacgcccct catgaattgg
    11461 tgcgcttccc cctggaaaga tgatccgcct gtatatttct tgtgtctatt tgttccacgt
    11521 tgtttcatac agtgcaccga acggtgtagt cactggtgta gtcactttgc gatttttggc
    11581 actttttgcg gtgaaaatta tttaattaaa tcaacgtgtt atgtgaaata agttatatat
    11641 aacagcaata aataatgcga gaataaacgg cacgatgatc tcggcggcaa aacggatacc
    11701 gcagagaata atgaccagca ttcccaacat aatgacgatt tttaggccat tgagcgtgat
    11761 gatcggcttt gccatgcttg tccccgtttt ttcttccttt
//
LOCUS       DAZGJW010000137        11740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZGJW010000137 DAZGJW010000000
VERSION     DAZGJW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.137"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..193
                     /locus_tag="ACHKIK_003935"
     CDS             <1..193
                     /locus_tag="ACHKIK_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085014.1"
                     /translation="KAIRQDGLFALVKKMPDATLRVLYSHICQIQQRIRLPQLAHLHT
                     CSPHQKFILKLLNNHFPAN"
     gene            310..1608
                     /gene="kgtP"
                     /locus_tag="ACHKIK_003936"
     CDS             310..1608
                     /gene="kgtP"
                     /locus_tag="ACHKIK_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO9085015.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1605..1928)
                     /gene="yfiM"
                     /locus_tag="ACHKIK_003937"
     CDS             complement(1605..1928)
                     /gene="yfiM"
                     /locus_tag="ACHKIK_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO9085016.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1974..3329)
                     /gene="pssA"
                     /locus_tag="ACHKIK_003938"
     CDS             complement(1974..3329)
                     /gene="pssA"
                     /locus_tag="ACHKIK_003938"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO9085017.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3443..6103)
                     /gene="pat"
                     /locus_tag="ACHKIK_003939"
     CDS             complement(3443..6103)
                     /gene="pat"
                     /locus_tag="ACHKIK_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO9085018.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6135..6833)
                     /gene="tapT"
                     /locus_tag="ACHKIK_003940"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6135..6833)
                     /gene="tapT"
                     /locus_tag="ACHKIK_003940"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO9085019.1"
                     /translation="MTENGVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMASDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6902..7321)
                     /gene="trxC"
                     /locus_tag="ACHKIK_003941"
     CDS             complement(6902..7321)
                     /gene="trxC"
                     /locus_tag="ACHKIK_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO9085020.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7528..8565
                     /gene="yfiF"
                     /locus_tag="ACHKIK_003942"
     CDS             7528..8565
                     /gene="yfiF"
                     /locus_tag="ACHKIK_003942"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO9085021.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8613..9302)
                     /gene="ung"
                     /locus_tag="ACHKIK_003943"
     CDS             complement(8613..9302)
                     /gene="ung"
                     /locus_tag="ACHKIK_003943"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO9085022.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9607..9990
                     /gene="grcA"
                     /locus_tag="ACHKIK_003944"
     CDS             9607..9990
                     /gene="grcA"
                     /locus_tag="ACHKIK_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO9085023.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10046..10633)
                     /gene="eamB"
                     /locus_tag="ACHKIK_003945"
     CDS             complement(10046..10633)
                     /gene="eamB"
                     /locus_tag="ACHKIK_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO9085024.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10736..11617
                     /gene="yfiE"
                     /locus_tag="ACHKIK_003946"
     CDS             10736..11617
                     /gene="yfiE"
                     /locus_tag="ACHKIK_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9085025.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNASSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
BASE COUNT         2921 a         2932 c         3036 g         2851 t
ORIGIN      
        1 aaaggccatc cgtcaggatg gcctttttgc gttggtgaaa aaaatgcctg atgcgacgct
       61 gcgcgtctta tactcccaca tatgccagat tcagcagcga atacggcttc cccaacttgc
      121 ccacctccat acgtgttctc ctcaccagaa atttatcctt aagctcctca ataaccattt
      181 tcctgctaac taaattcatg gttaaggttg cataatgata cgcaacaaat gtataacatt
      241 ttctttacca aaagaaataa acaaaagcga ccgacaaaag catcggatta cggcaggaga
      301 cataatggca tggctgaaag tactgtaacg gcagacagca aactgacaag tagtgataca
      361 cgtcgccgca tttgggcgat tgtgggggcc tcttcaggta atctggtcga gtggttcgat
      421 ttctatgtct actcgttctg ttcactctac ttcgcccaca ttttcttccc ttccgggaac
      481 acgacgactc aactactaca aacagcaggt gtttttgctg cgggattcct gatgcgccca
      541 ataggcggtt ggctatttgg ccgcatagcc gataaacatg gtcgcaaaaa atcgatgctg
      601 ttatcggtgt gtatgatgtg tttcggatcg ctggttatcg cctgcctccc aggttatgaa
      661 actataggta cgtgggctcc ggcattattg cttctcgctc gtttatttca gggattatcc
      721 gttggcggag aatatggcac cagcgccacc tatatgagtg aagttgccgt tgaagggcgc
      781 aaaggttttt acgcatcatt tcagtatgtg acgttgatcg gcggacaact gctagcccta
      841 ctggttgtcg tggttttaca acacaccatg gaagacgctg cactcagaga gtggggatgg
      901 cgtattcctt tcgcgttagg agctgtgtta gctgttgtgg cgttgtggtt acgtcgtcaa
      961 ttagatgaaa cttcgcaaca agaaacgcgc gctttaaaag aagctggatc tctgaaagga
     1021 ttatggcgca atcgccgtgc attcatcatg gttctcggtt ttaccgctgc gggctccctt
     1081 tgtttctata ccttcaccac ttatatgcag aagtatctgg taaatactgc gggaatgcat
     1141 gccaacgtgg cgagtggcat tatgactgcc gcattgtttg tattcatgct tattcaacca
     1201 ctcattggcg cgctgtcgga taagattggt cgccgtacct caatgttatg tttcggttcg
     1261 ctggcagcca tttttaccgt tcctattctc tcagcattgc aaaacgtttc ctcgccttat
     1321 gccgcttttg gtctggtgat gtgtgccctg ctgatagtga gtttttatac atcaatcagt
     1381 ggaatactga aggctgagat gttcccggca caggttcgcg cattaggcgt tggcctgtca
     1441 tatgcggtcg ctaatgctat atttggtggt tcggcggagt acgtagcgtt gtcgctgaaa
     1501 tcaataggaa tggaaacagc cttcttctgg tatgtgacct tgatggccgt ggtggcgttt
     1561 ctggtttctt tgatgctaca tcgcaaaggg aaggggatgc gtctttagtg acgggtcagt
     1621 tgccagacgg tatagccggt gcttgcaccg gcgacatccc aggccaaatc cttccagctc
     1681 cagccgctcc cttcggggcg gctatcccaa agctcttttg acgcccccaa actgacagag
     1741 aacatcaatc caaacatggc actgcgatcc cggctcatcc cctgatgctg tgaatattca
     1801 tttccggcgg cggaaagcat cgccgaggcg ataaagtgtt gagctttatc ctgcccgctc
     1861 cagctatcgt tagccatatg gctgcatcca gaaagtaaca atagcgaaca gacaaaaaga
     1921 atacgcaaaa cgtggcctcc aaaaaacaaa ccccgtacag gacggggttg tgattacagg
     1981 atgcggctaa ttaatcggtc gatgcggata cggcgcaaac ggcggatgag tttacgaacc
     2041 ttcaccggat aatcggcaat actttgcaga tcgcgatagt gcttaacgat ggtggtatgc
     2101 tcgcggatca gctccagttc tttctctcgc tgcggcgcaa gctccagttg tggatcgtgg
     2161 atcaaaatgg cgttttccag atccagacgc caggcgcgcg ggttcaggtt attaccggtg
     2221 atcaacatcc acttatcatc aacccacatc ccttttaggt gataggtgtt gtcgtcatct
     2281 ttccataacc gaaccactag ctggtcagta ttgacgtaat actgcaaacg gctcaggaaa
     2341 cgacgcagat tgatctcata gagataaggc aatgcgccaa ttatcttgaa aggttcatct
     2401 tccggaatgt agaagtcgtt cgcggtttta tcaccaacaa taatttcgac ctttttccct
     2461 tcgcgcagca actggataat attgcgcaca aggattgctg gcaggttgaa gtatggcgta
     2521 cagatggtta gtttctgctc ggcacaaggc ataagatgga aaatggtctt gttcaacaga
     2581 ctcgatttcc ccagccccac tagcggcgtt acggaaagct gatcgttgtc ggcatcgccc
     2641 tggaaatgat aagcggcatc acgcagctcc tggcggaaca gacgaatatc gttcttgatt
     2701 tccgggcttt ttggccgatt aacatcatcc agacgattaa cgccgcggcc attcataata
     2761 ttctgtgtaa cccattcaaa cataatgtct gacatcttac ggttacggat aagatgataa
     2821 cggtcgtagc gatatttatc gtgctgatgc aggtaaacat cgttcaggct ggcaccgcta
     2881 taaagtacgc tatcgtcgat gataaagcct ttaaagtgca gaacaccaag ggcttcacgg
     2941 gtattgattg gaacgccata aaccggaaca tctacgcccg gattttcctg cgccatgcgg
     3001 cagtaccagt cagcgttagt gttagatgcc gcagcgccaa tgcgtccacg ttgtgcacga
     3061 tgccagtcga ccagcacccg cacatccagt tccggacgct gccttttagc ttcatacaac
     3121 gcgttcagaa tgcctttgcc accgtcatcc tgttcgagat acagggcgac aatgcaaatg
     3181 cgctgcttcg cgctggctat tttttccagc agcgtctccc ggaagtcggc gggagcgtaa
     3241 aagaaatcga catcatcaac tgattgagaa atcttgggta gttgggcaag gtgttgttga
     3301 tgtttattac gcttaaattt tgacaacatc acagtgcatt tcttctctgt tcattgaagg
     3361 gtcctctgtg caatgcagac gacataagcg ggcaataata acaccagtcc cgattaagtg
     3421 gtcaacattt ccagtacctt actcatgatt cctcgcgttg ggcaagattt agcgtaagcc
     3481 caacgatccc ctcttcgagc tggatatcaa cgttaaaccc gagcttgcgg gctagcgcca
     3541 ccatgccacg attgtttggc atcgtaatac cattcagacg ttgtagtccg tgatctcgcg
     3601 tataggtaat caacttttcc attaagcgtc gacctaagcc taaccctttg agatccgagc
     3661 gaaccagcac agcaaattcg gcatcgatgt tatcaggatc ggaaatcgca cgcgtgacgc
     3721 cgaggatctc ttccgtttga tcaatacgtc gtaccgctac aaacgccatt tcccgatcgt
     3781 agtcgatctg tgtcatgttg gctaaatctt catgggtaaa ttcgttgatc tcgctaaagt
     3841 agcggtaata aagatcttct ttggtgactc gcgaaatgaa ttgctgaagt tgtggctcat
     3901 cttctggcaa aatcgggcgg aacaagcagc gttcaccgtt tttcaattct acccattctt
     3961 ccagctgatg cggataaggg cgcactgcca gccgactctc gttatcgcct tcaaacggcg
     4021 agatatccag cgtgacatcc agcgcggtaa attcactgcc agaagccagc aaaggatgaa
     4081 tatccagacg ctgaatttcc gggcaatcga caatcaagtt ggaaacctgc accagaagct
     4141 ggctcaagcc tgcaacatcc aatgggcgta gcgcactgcg tgcacgaatc tttttacttt
     4201 tgatcccctg aataaccaga tagcgggcca ggttcatgtt cagcggcggt aacgcgacga
     4261 cggcttgatc ttcaggacgc cactccacac cgccttcgcc cagcatgatc aacggcccga
     4321 aaaccggatc gtgctcaacc acaacccgca actcctgagc accagcacgg ttagccatac
     4381 tttgcaccaa caggccgtgg acccgtgcct gcggccaggc catttttacg cgatcgaaaa
     4441 tggcgtttgc cgcttgctgg acttcattgg ctgtacgcag gtagagcatg acgccctgaa
     4501 cttccgattt atgtggaata tccggcgaac gcaatttcag cgccaccgga taaccaatct
     4561 gttcggcaat atgcaccgct tcggtgctat cgctggcaat ccaggtgggg agcgtgttca
     4621 tgccatatgc ttgcaggata ggctgaactt catgggtatc gagtgacgtt gctccttcgg
     4681 caatcgcctg ttgcaacaga agatgcgctt ctgcggtatt ggaggtcaga ttgctgggca
     4741 acgccggcgt ttcgcgtagt tgcttctgat tacgccggta ctccaccata tgcataaaag
     4801 cagtgatggt tccttccggg gtacggtagg tcggcagccc ggcttcgctg aataatcgtc
     4861 gtgcctcttg cgaggaatgt tcgccacacc agttcgtcag cagagaaaca tacttgctgc
     4921 ggggatgatg ctttaccgct tcaattaata cttgcgcgct ttctgttgcg ggagcagcgg
     4981 cgctgggcga atgaataacc atcagcgcat caaaatcctg gctgtggagc agaatgtcca
     5041 gcgttttaat atagtgctca ctgctggcgt catcgcgcag atcgagcggg ttagagattg
     5101 ccacatgtcc tggcagtgca tcgcgcagtt tctggcaggt ttcttcgctt agtgttgcca
     5161 gcttgccatt gcgtgaccat aaggcatcaa gcgccagcgc ggcaggcgca gcaccgttgc
     5221 tgataatcat caaccggtcg ccacgcagcg ggcgcatatg gctaagggtt tccaccgccg
     5281 aaaacagttc gtgggtgtcc tgtacccgca acaaaccggc acgctgaata gccgcatccc
     5341 acgccggatc cattcctgcc gtggtgttca acagacgttg agccgccggg ctacgtccgc
     5401 ttttaatcac cagaatcggt ttgttgcgag aggcactacg ggccgccgaa acaaagcgtc
     5461 gcgcgtcgct taactgttcg agatagagca ggatggcgct ggttttactg tcgcgtgcca
     5521 gatagtcaag caattcatca acgtcgatat ccaggctgtc gccgagcgca ataaagtagg
     5581 aaaagcccat cttacgctgt tgcgcccagt cgaggatggt gttggagacg gcagccgatt
     5641 gcgaaataaa cgccagcttg ccgcgtttaa tcggcacagg cgaaaagctg gcattcagac
     5701 cttgccaggg agccagtaaa cccagactgt ttggtccaag caggcgcatg ttatggcgca
     5761 gggcgcaggc gcggagatct tcgtgttgcg atgccggggc ggaaagaata atgcaggttt
     5821 tacagccttt ctcgccgagc tcttccagaa gagcaagatt acggctggca ttggtacata
     5881 aaaccgcaag gtcgggtgta aagggcaagc tggcaatatc cggccaggcc aacacaccca
     5941 acaccgcttt ccaggctggc gtcaccggga gtaccggtcc gttaaagcct cccgccagca
     6001 ggttacgcat catcaggtaa cctgcgcgat tgggtttcat cgacgcgcca attaccgcta
     6061 tcgattttgg tcgcagtagt gcttccagtc ctcgctgact cataccggtc tcccgcagaa
     6121 gtgaccgaat gattttaaac gctttctaac tgttctgctg tgatgctacc cagatgttgc
     6181 gtttttcctg ccagatagcg tgttttaaag cgggtaaaat gctcgcctaa ccctgctgcc
     6241 gccccggtat cgctggccat atctaacagt gcgatggcta cctcggcagt acaatattgg
     6301 cctgcagcct gggcttcacg caggcgatag gcagaaagcc gggaaagatc gacggaaatg
     6361 acgggaagat tatccagata cggactttta cgaaacatct tgcgagcttc cggccaggta
     6421 ccatcgagca tgataaacag cggtggctta ccggcaggtg gtgtgaagat cacttcccgt
     6481 tgctcatcag catacgaagc gggaaagacc accattggct gatagtccgg gttttgcacc
     6541 aactccagca aatcctgcga gggttcggta cgcgaccatt gaaacgcaac ggtatcaggc
     6601 aaaatatcag caatcagacg cccggtatta ctgggcttca ttggctcggt gtcgaacatc
     6661 agcaaacaga agcgactttt tgcttgtgct ggggtaattg tcgaacagag acataatttc
     6721 tctggcaaaa gacagcgttg gcagcgacga acgcgattac cgcgggcaag aaaaggacgt
     6781 gttgcgcgcg caatacgctc ggcgcgtaac tggagaacac cgttttcggt cataagagag
     6841 cgtcgaaaaa acgccattgt cgcagaggag aaaacggggc acaagatgcg ccccggtaag
     6901 attaaagaga ttcgttcagc cagctatcga acggcgcttt cggtactgcg ccattaagca
     6961 tgtcgacaac ctgaccgttt ttgaaaatca tgatcgtcgg tatactacga attccaaagc
     7021 gactgctcaa ttcacgttca gcttcggtat tcactttcac aaagcgcact ttaccgctac
     7081 gctcttgcgc gacatcttca aaaattggtg cgaaattacg gcaggggccg caccacggtg
     7141 cccagaagtc gatcaccaca ggtagatcat ccttcagcaa tttgtcgagt gtttcaccgg
     7201 tcgcattaat cacctctccg tcaaacaagt cgtgaccgca gcgtccgcat tttgccgcat
     7261 cttcgatccg atcgtcggga atgcgattga tggcctgaca atgggtacaa acggtattca
     7321 taactaacct cgggatgagt aaggagactt atcggcaacg acgccggtat gtttctaata
     7381 tgttacatat tatcggggac attgttccag acaacaatct gttttattca ataggtatga
     7441 tagtcgcagg ctttggtacg aaatcatggc gaaccgcgcg gacttcgggt aatctgcgcg
     7501 cttcgcgcag cgctggtgga gaaaagcatg aacgatgaaa tgaaaggtaa aagcggcaag
     7561 gtcaaagtga tgtatgtccg cagtgatgat gattctgata aacgtaccca caacccgcgt
     7621 accgggaaag ggggcgggcg tccaggaaaa tctcgtgctg acggtggccg tcgccccgcc
     7681 cgcgatgaca aacagagtca gccccgtgac cgcaagtggg aagattcgcc gtggcgcacg
     7741 gtttcccgcg cgccgggtga tgagacgccg gaaaaggccg atcacggtgg catcagtggt
     7801 aaaagtttta ttgatccgga agtgttgcgt cgtcagcgtg cggaagaaac ccgcgtctac
     7861 ggcgaaaatg catgtcaggc acttttccag agccgtccgg aagcgattgt tcgcgcctgg
     7921 tttatccaga gtgtaacgcc gcgttttaaa gaagccttgc gctggatggc agcaaaccgc
     7981 aaagcgtacc atgtggtgga tgaagcggaa ttgacaaaag cgtcaggcac ggaacatcac
     8041 ggcggcgttt gcttcttgat caaaaagcgt aacggtacaa ccgtgcagca gtgggtaagc
     8101 caggcaggcg cgcaggattg cgttctggca ctggaaaacg aatctaaccc gcataacctg
     8161 ggtggcatga tgcgcagctg cgcgcacttc ggcgtgaaag gtgtcgtggt gcaggatgcg
     8221 gcactgctgg agtcgggggc ggctatccgt accgcagaag gcggcgcaga gcacgttcag
     8281 ccgattactg gcgacaacat tgttaacgtg ctggatgatt tccgtcaggc aggttacacc
     8341 gtagtgacca cttccagcga gcagggtaaa ccgctgttca aaactagtct gccagcgaaa
     8401 atggtactgg tgctgggtca ggaatatgaa gggttaccgg atgccgcacg cgatccgaac
     8461 gatctgcgcg tgaagattga cggtacgggc aacgttgccg ggctgaatat ctctgtcgca
     8521 accggcgttt tgcttggtga atggtggcgt cagaataaag cctgataaat cagccgggtg
     8581 gcaactctgc catccggcat ttccccgcaa atttactcac tctctgccgg taatactggc
     8641 atccagtcaa tcggcgtctc gccacgttgt tccagccact gatttgccag cacaaaatgg
     8701 ttgcagccaa agaatccacg atgcgccgaa agcggcgacg gatgcggtgc tttcagtaca
     8761 tgatggcgtt gcttatctat aatcgcccct ttcttttgcg catgtgatcc ccacaacaaa
     8821 aacaccacgc cttcgcggtg ctggttaatc aggctgatga ctttatcagt aaacgtctcc
     8881 caaccgaggc tggcgtggga atgcgcctga cctgcgcgaa ccgtcaacac agtattgagc
     8941 agcagaacgc cctgacgcgc ccagctttcg agatagccat gattagggcg ggtgaagccc
     9001 ggaatagtat tttccagctc tttatacata ttcaataatg acggaggaat ggcaatgccg
     9061 ggacgaacgg aaaatgccag accatgcgcc tgtcccggtc cgtgataagg atcctggccg
     9121 agaatcacca ctttaacgtc acccaactcg gtaaagcgga acgcgttaaa gacatctttt
     9181 tgtggtggat agatagtgac gccggactgc cgctcgctgg cgacggtctg aagggtatta
     9241 agaaaataag gttgttgctt ctcttcagcc agcacgtcat gccaggttaa ttcgttagcc
     9301 atctcgctct cctgcgaatc ttcaatccgc ctagcttaac tgcttctttc tacagaacaa
     9361 aatcatgctc attttttaga cttaaacgag tgcaaaaaaa tgtaaattgg taaaatttca
     9421 cacccgcata agttgatgta aaacaataaa atccatttac gaccacaatc gatgtgtggt
     9481 ttttattgat ttaaatcaaa gaatcaaggg tggttgagga gtatatatac actcaagcaa
     9541 caatggtttt accaattggc cgcgacaggc tgaacaaatc aaataatttt gccggggagg
     9601 catcacatga ttacaggtat ccagattact aaagccgcta acgacgatct gctgaactct
     9661 ttctggctgc tggacagcga aaaaggcgaa gcacgttgca tcgttgcaaa agcaggttat
     9721 gcagaagatg aagtggttgc agtaagcaaa ctgggtgaca ttgaataccg tgaagttcca
     9781 gtagaagtga aaccagaagt tcgcgttgaa ggtggtcaac acctgaacgt taacgttctg
     9841 cgtcgtgaaa ctctggaaga tgcagttaag catccggaaa aatatccgca gctgaccatc
     9901 cgtgtatccg gttatgcagt tcgctttaac tctctgactc cggaacagca gcgcgacgtt
     9961 atcgctcgta cctttactga aagcctgtaa ggcttaagta gcaagttact aagcggaaga
    10021 gggcgacctc ttccgctttt tttcgttaat agaaaatgcg taccgcgcaa tagaccagca
    10081 acagggcaag cacgatattt aactggcgac catactggcg aaacaatcgc tgaaacagat
    10141 gccccgccag cgcccagcac acattgccaa acgtcccaat catcgccagc aaaacgctga
    10201 cgccaactac ccagcttagc gcctgtgttt gcggcagaac aaacgtcgac agtgccgtaa
    10261 caccgtacaa aatgattttg acgttcacaa actgcaaagc aaagctggcc caaaagctga
    10321 ttggttttgc ctgaagtccg tcttcctttg ttgggctggt ggcgattttc cacgccagcc
    10381 agacaatata tgccgccccc gcccaactca aaaggtgtac cgctgccggg tcaatcactg
    10441 ccagtgaaaa tgaaatgccc gcacacagta acatcacaat caaaaatccc agactcatcc
    10501 ctgccagcac gcgggtactt tgacgaaatc catgcgacgt agcagagcta agggcgagaa
    10561 tattgttcgg tcctggcgtc atagcggtaa tcagggtgta agtccaaaaa gcacttaaaa
    10621 gggtcggtgt cacttgttat ctcctctgtt ttggtagaca gtacccgaca cggaacattt
    10681 aaaaaaatga atgtttttat attctagatt cgatattttc gataggtttg gggttatgga
    10741 tctgcgccgt tttattacgc ttaaaaccgt ggtggaagag ggttcctttt tgcgagcttc
    10801 gcaaaaattg tgctgtacac aatcgacggt gacttttcat attcagcagc ttgagcagga
    10861 gttttcagtc cagttatttg agaaaattgg ccgacggatg tgccttaccc gcgaaggaaa
    10921 aaagttgctg ccgcacattt atgaacttac ccgagtgatg gatacgcttc gtgaggctgc
    10981 caaaaaggag tcagatccgg acggggaact acgcgttgtt tcgggcgaaa cgttgctctc
    11041 ttaccgaatg ccgcaggtgt tgcagcgttt tcggcaacgc gcaccaaaag tgcgtttatc
    11101 gttgcagtcg ctgaactgtt acgtgatccg tgatgcgcta ctgaatgatg aggccgatgt
    11161 cggcgtcttt tatcgcgtag ggaatgatga tgccctgaac cgacgagagt tgggtgaaca
    11221 atcgctggtg ctggtggctt caccgcaaat tgcagatgtc gattttactg agccgggaag
    11281 acataacgcc agtagcttta ttatcaacga accgcaatgt gtcttccggc agatatttga
    11341 gagcacgctg cgtcagcggc ggatcacggt ggaaaacacc attgagctgt taagcattga
    11401 aagtatcaaa cgttgtgtcg cggcgaatat cggtgtcagt tacttgcccc gctttgcggt
    11461 ggaaaaagag cttgagagcg gagaattaat tgaattgcct tttggcgaac agtcgcagac
    11521 cattacggcc atgtgcgcac accatgctgg aaaagcggtt agcccggcga tgcacacttt
    11581 tattcagtgt gttgaagaga gttttgtggc tggataaaaa taatgtcggg attaaacccg
    11641 gcatttaact tgtcgacaaa gtgcgcttta ttcatgccgg atgcggcgtg aacgccttat
    11701 ccggcctaca agatcgtgca aattcaatat attgcatgag
//
LOCUS       DAZGJW010000138        11600 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZGJW010000138 DAZGJW010000000
VERSION     DAZGJW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.138"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            262..1650
                     /gene="narU"
                     /locus_tag="ACHKIK_003947"
     CDS             262..1650
                     /gene="narU"
                     /locus_tag="ACHKIK_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO9085026.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1732..5472
                     /gene="narZ"
                     /locus_tag="ACHKIK_003948"
     CDS             1732..5472
                     /gene="narZ"
                     /locus_tag="ACHKIK_003948"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO9085027.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5469..7013
                     /gene="narH"
                     /locus_tag="ACHKIK_003949"
     CDS             5469..7013
                     /gene="narH"
                     /locus_tag="ACHKIK_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO9085028.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7013..7708
                     /gene="narW"
                     /locus_tag="ACHKIK_003950"
     CDS             7013..7708
                     /gene="narW"
                     /locus_tag="ACHKIK_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO9085029.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRSDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7705..8385
                     /gene="narI"
                     /locus_tag="ACHKIK_003951"
     CDS             7705..8385
                     /gene="narI"
                     /locus_tag="ACHKIK_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO9085030.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKREMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8464..9357
                     /locus_tag="ACHKIK_003952"
     CDS             8464..9357
                     /locus_tag="ACHKIK_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO9085031.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9453..10298)
                     /gene="nhoA"
                     /locus_tag="ACHKIK_003953"
     CDS             complement(9453..10298)
                     /gene="nhoA"
                     /locus_tag="ACHKIK_003953"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO9085032.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10471..11040
                     /locus_tag="ACHKIK_003954"
     CDS             10471..11040
                     /locus_tag="ACHKIK_003954"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO9085033.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11044..11271)
                     /gene="pptA"
                     /locus_tag="ACHKIK_003955"
                     /pseudo
     CDS             complement(11044..11271)
                     /gene="pptA"
                     /locus_tag="ACHKIK_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
     gene            complement(11370..>11600)
                     /locus_tag="ACHKIK_003956"
     CDS             complement(11370..>11600)
                     /locus_tag="ACHKIK_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9085034.1"
                     /translation="WHVENKLHWRLDVVMNEDDCKIRKGNAAELFSGIRHIAINILTN
                     DKVFKAGLRRKMRKAAMDRNYLASVLAGRRLS"
BASE COUNT         2726 a         3025 c         3047 g         2802 t
ORIGIN      
        1 ccctttagat tcacgatctg aagggttgaa acatgacata cgttcaaaga ttaacccccg
       61 tttgaaggtt agcttgttag ttacatttag taacacatat ctggctactt aatccccatt
      121 ctgcccccgc ggttacgctt tcttatctat tctttcaatc agcacttcac ccaattcatc
      181 cttaattcta ataactggct attgcgcctt acctcctgtt ttgtcttttt tcattaccaa
      241 tgtgtgcatg tgaggaacaa tatggcactg caaaatgaga aaaatagtcg ttatctttta
      301 cgcgactgga aaccagaaaa tccggccttc tgggaaaata aaggaaaaca tattgctcga
      361 agaaacctct ggatatcagt cagttgtcta cttcttgcct tctgtgtctg gatgctattt
      421 agcgcagtta ccgttaatct caataaaatc ggttttaatt ttactaccga tcaactcttt
      481 ttattaaccg cattaccctc cgtttctggc gcattattgc gtgttcccta ctcctttatg
      541 gtgcctatat tcggtggacg ccgatggacg gtttttagta ctgcaatcct gattattcct
      601 tgcgtctggc tcggaattgc cgtgcaaaat ccgaatactc cttttgggat atttatcgtt
      661 atcgctttgc tatgcggttt tgcaggtgca aactttgctt cgagcatggg caatatcagt
      721 ttcttctttc caaaagccaa acaagggagc gctcttggga ttaatggcgg attaggaaac
      781 ttaggtgtaa gtgtaatgca gctggttgca ccgctggtca tttttgtacc tgtatttgcc
      841 tttctcggcg tcaatggcgt accgcaggcc gacggttcgg tgatgtcgct ggcgaatgcc
      901 gcatggattt gggtaccgct actggcgatt gccacgatcg ccgcatggtc agggatgaat
      961 gatatcgcca gttcacgcgc ctcaattgcc gaccagctcc ctgtcttaca acgcctgcat
     1021 ctctggctgc tgagcctgct ttaccttgcc accttcggtt cgtttatcgg tttttctgcg
     1081 ggttttgcca tgctggcaaa aacccagttc ccggatgtga atattctgcg cctggcgttc
     1141 tttggcccat ttatcggtgc catcgcgcgg tcggttggtg gtgctatttc cgataagttc
     1201 ggcggcgtgc gggtgacgtt gatcaacttt atttttatgg cgattttcag tgccctgctg
     1261 ttccttacct taccgggcac aggctccggt aatttcatcg ccttttacgc cgtatttatg
     1321 gggctgtttc tgactgcggg tctgggaagt ggttctactt tccagatgat cgccgtcatc
     1381 tttcgccaga taaccattta tcgggtaaag atgaaaggcg gtagtgatga gcaagctcag
     1441 aaagaagccg tcaccgaaac ggcggcggct ctgggcttta tctcagccat tggcgcagtg
     1501 ggcggctttt ttattccgca ggcgtttggc atgtcgctca atatgaccgg ctctccggtc
     1561 ggcgcgatga aagtgttttt aatcttctac atcgtttgtg tgctgctgac ctggctggtt
     1621 tatggtcggc ggaagttcag ccaaaaataa gagatcgatg cctgatgtac aaccgtgtcg
     1681 catccgacaa ggcatcagat cccaattcat tcctggagca ggagtcatgt catgagtaaa
     1741 cttttggatc gctttcgcta cttcaaacaa aagggcgaaa cctttgccga tggtcacgga
     1801 caggtgatgc atagcaaccg cgactgggag gacagctatc gccagcgttg gcagttcgac
     1861 aaaatcgtgc gttccaccca cggtgttaac tgtacaggct cctgtagctg gaaaatctac
     1921 gttaaaaatg gtctggtgac ctgggaaatc caacagaccg actacccgcg cactcgccct
     1981 gacctgccca atcatgaacc tcgcggctgc ccgcgtggcg caagttactc ctggtatctt
     2041 tacagcgcta accgcctgaa atacccgctc attcgtaaac gactgattga actgtggcgc
     2101 gaagccctca agcaacacag cgatccggta ctggcgtggg catcgattat gaacgatccg
     2161 caaaagtgcc tgagctacaa acaagtgcgt gggcgcggcg ggtttatccg ctccaactgg
     2221 caggaactaa accagctgat tgccgccgct aacgtctgga ccatcaaaac ctacggcccg
     2281 gatcgcgttg ccggtttctc gccgatcccg gcgatgtcga tggtttctta cgccgccgga
     2341 acgcgttatc tgtcgctgct tggcggcacc tgtttaagtt tctacgactg gtattgcgac
     2401 ctgccgcccg cctcgccgat gacctggggc gagcaaaccg acgtaccgga atctgccgac
     2461 tggtataact ccagctacat catcgcctgg gggtctaacg taccgcagac acgtacgccg
     2521 gacgcccact tctttaccga agtacgctac aaaggcacta aaaccatcgc cattacccct
     2581 gactactctg aagtggccaa attgtgcgac cagtggctgg caccgaaaca aggcactgat
     2641 agcgccctgg cgatggcaat gggccatgtg attttaaaag agtttcatct cgataatccc
     2701 agcgactact ttatcaacta ctgccgccgc tacagcgaca tgccgatgct ggtaatgctg
     2761 gagcctcgcg acgatggtag ctacgttccc gggcgcatga tccgcgcatc tgacctggtg
     2821 gatggactgg gcgaaagcaa caatccgcag tggaaaaccg tagcagttaa taccgcaggt
     2881 gaattggtag tgccgaacgg ttcgattggt ttccgctggg gagaaaaagg caaatggaat
     2941 ctggaatcca ttgccgccgg tacggaaacc gaattgtcgt taaccctgct cggtcaacat
     3001 gacgctgttg caggcgtggc cttcccctac tttggcggca ttgaaaatcc gcattttcgc
     3061 agcgtaaaac acaatccggt gctggtgcgc caattgcccg ttaaaaacct gacgttagtc
     3121 gatggcaaca cctgtccggt ggtcagcgtt tatgatttgg tactggcgaa ttacggcctc
     3181 gatcgcgggc tggaagatga aaacagtgcg aaagattacg ctgaaatcaa accgtacacc
     3241 ccagcctggg gtgagcaaat taccggcgtg ccgcgccagt atattgaaac catcgcccgt
     3301 gaatttgccg ataccgccca taaaacgcat gggcgctcga tgattatcct cggcgcaggt
     3361 gttaaccact ggtatcacat ggacatgaac taccgcggga tgatcaatat gctgatcttc
     3421 tgcggttgtg tcgggcaaag cggtggcggc tgggcacact atgtcggcca ggaaaaactg
     3481 cgcccacaaa ccggctggtt gccgctggcc tttgcgctcg actggaaccg accaccacgc
     3541 cagatgaaca gtacctcgtt tttctacaat cattccagcc aatggcgcta tgaaaaagtc
     3601 tctgctcagg agttactttc accgctcgcc gatgccagta agtacagcgg tcatctgatt
     3661 gatttcaacg ttcgcgccga acgtatgggc tggctacctt ctgcgccgca gctggggcgt
     3721 aacccgctcg ggattaaagc tgaagccgac aaggccggat tatcccccac agaatttacc
     3781 gcccaggcgc tgaaatcggg cgatttacgt atggcctgcg aacaaccaga tagcggcagc
     3841 aatcatccgc gtaatttgtt tgtctggcgc tctaacctgc ttggctcctc cggcaaaggc
     3901 cacgagtata tgcagaagta tctgctgggg accgaaagcg ggattcaggg cgaggaactc
     3961 ggtgccagcg acgggatcaa accggaagaa gtcgagtggc aaactgcggc gattgaaggc
     4021 aagctcgacc tgctggtgac gctcgacttc cgcatgtcca gtacctgcct gttctccgat
     4081 atcgttctgc ccaccgccac ctggtacgaa aaagacgata tgaacacctc ggatatgcat
     4141 ccgtttattc atccgctttc tgcggcggtc gatccggcgt gggaatcacg cagcgactgg
     4201 gaaatctaca aaggtatcgc caaagcattt tcgcaagtgt gcgtgggtca tcttggcaaa
     4261 gaaaccgacg tggtattaca acccctactg catgactctc cggcagagct ctcacagccg
     4321 tgtgaagtgc tcgactggcg caaaggcgaa tgcgatctga ttccaggcaa aaccgcgccg
     4381 aatattgtgg cggtggagcg cgactaccct gctacgtatg aacgctttac ctcgctcggg
     4441 ccattgatgg acaaacttgg caatggcggt aaagggattt cgtggaatac gcaggatgaa
     4501 atcgatttcc tcggcaaact caattacacc aagcgtgatg gcccggcaca ggggcgtccg
     4561 ctgattgaca ccgccattga cgcttcagaa gtgattctgg cactggcacc ggaaaccaac
     4621 ggtcatgttg cagtcaaagc gtggcaggcg ctgggcgaga tcaccgggcg cgaacatacc
     4681 catctggcgc tgcacaaaga ggacgagaag attcgctttc gcgatattca ggcgcagccg
     4741 cgtaaaatta tctccagccc cacctggtcc ggtctggaaa gcgatcacgt atcctataac
     4801 gcgggataca ccaacgttca tgagttaatt ccgtggcgca cgctgtcggg acgccagcag
     4861 ctctatcagg atcatccgtg gatgcgtgct tttggtgaaa gcctggtggc ttatcgcccg
     4921 cctatcgaca cccgtagcgt cagtgagatg cgccagatac cgccaaacgg cttcccggaa
     4981 aaagcactta acttcctgac gccgcaccag aaatggggca ttcactcaac ctacagtgaa
     5041 aacctgctaa tgctgacgct ctctcgcggt ggaccgattg tctggatcag cgaaacagat
     5101 gcccgagaac tgaccattgt cgataacgac tgggtggaag tattcaacgc caacggcgcg
     5161 ctgactgccc gcgcggtggt cagccaacgt gtaccgccgg gcatgaccat gatgtatcac
     5221 gcccaggaac gcattatgaa tattcctggt tcggaagtaa ctggcatgcg cggcggcatt
     5281 cataactcgg tcacccgcgt ttgcccgaaa ccaacgcata tgattggcgg ttacgcgcag
     5341 ctggcctggg gctttaacta ctacggcacc gtcggatcga accgcgatga gttcatcatg
     5401 atccgcaaga tgaagaacgt taactggctg gatgatgaag gtcgcgatca ggtacaggag
     5461 gcgaaaaaat gaaaatccgt tcacaagtcg gcatggtgct taacctcgac aaatgtatcg
     5521 gctgccatac ctgttcggtg acctgtaaaa acgtctggac cgggcgcgaa ggcatggagt
     5581 acgcatggtt taacaacgtc gaaaccaaac cgggcattgg ttatccgaaa aactgggaag
     5641 atcaggaaga gtggcaaggc ggctgggtcc gcgatgtgaa tggcaagatc cgtccgcgtc
     5701 tgggcagcaa gatgggcgta ataaccaaaa tcttcgccaa cccggtggtg ccgcagattg
     5761 atgattacta cgaacctttc accttcgact acgaacattt gcatagcgca ccggaaggca
     5821 aacatatccc tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga
     5881 tctggggccc aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc
     5941 gcaacttcga ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt
     6001 acctgccgcg cctgtgcgaa cactgcctca atcccagttg cgtggcgacc tgcccaagcg
     6061 gcgctatcta caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg
     6121 gctggcgttt gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg
     6181 gcaagtcaga aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt
     6241 gctcagaaac ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc
     6301 gcattgagga agcggcgagc accgagcgcg aagttgacct ctatgaacgt cagtgcgaag
     6361 tattcctcga tccacacgat ccctcagtga tcgaggaagc cctgaaacaa ggtattccac
     6421 aaaacgtgat tgaagctgcc cagcgttcgc ctgtctacaa aatggcgatg gactggaaac
     6481 tggcgctacc gctgcaccct gaatatcgca ccctgccaat ggtctggtac gttcctccgc
     6541 tgtcgccgat tcagtcctac gcagatgcgg gcggtttgcc gaaaagcgaa ggcgtgctgc
     6601 ccgccatcga aagcctgcgt attccggtgc aatatctcgc caatatgttg agtgccggcg
     6661 ataccggtcc ggtactgcgg gcgctgaaac ggatgatggc gatgcgccac tatatgcgtt
     6721 cacaaaccgt ggaaggcgtt actgatactc gtgccatcga cgaagtaggc ctgagcgtcg
     6781 cccaggtcga agagatgtat cgttacctcg ccattgccaa ctatgaagat cgttttgtca
     6841 tcccgacgag ccatcgggaa atggcgggcg atgccttcgc agaacgcaac ggctgcggtt
     6901 ttacctttgg cgacggttgc cacggttcgg acagtaaatt caacctgttc aacagtagcc
     6961 gtatcgatgc catcaacatc accgaagtgc gcgacaaagc ggagggcgaa taatgcagat
     7021 cctcaaagtg atcggcctgt tgatggagta tccggacgag ctgttgtggg aatgcaagga
     7081 ggacgcgctg gcgttgatcc gcagcgacgc gccgatgctt acggatttca cccacaacct
     7141 gcttaacgcg ccgctgctgg ataaacaggc cgaatggtgc gaagtgtttg accgtggacg
     7201 taccacatcg ctgctgctgt tcgaacatgt tcatgccgag tcccgcgatc gcggccaggc
     7261 aatggtggac ctgctggcgg agtatgaaaa ggtcggcctg cagctggatt gtcgggaact
     7321 gcccgattat ctaccgctgt atctggagta tttaagcgta ctgcctgacg atcaggcgaa
     7381 agaaggattg ctcaatgttg cgccgatcct cgccctgctt ggcggtcgct taaaacaacg
     7441 cgaggcaccg tggtacgcgt tgtttgatgc tctgctgcaa ctggcgggaa gctccctttc
     7501 aagtgacagt gtcacaaaac aagtaaacag cgaagagcgc gatgacaccc gccaggcgct
     7561 tgatgcagtg tgggaagagg aacaggtgaa gtttattgaa gataacgcca cggcgtgtga
     7621 cagctcgccg cttaatcaat atcagcgacg ctttagccag gatgtcgcgc cgcaatatgt
     7681 cgacatcagt gcgggaggtg ggaaatgatt cagtatctga acgtcttttt ttacgatatc
     7741 tacccgtaca tttgtgcgac ggtgtttttc ctcggcagct ggctgcgcta cgactacggg
     7801 cagtacacct ggcgcgcctc ctcaagtcaa atgctcgata aacgcgagat ggtgatatgg
     7861 tcgaatctgt tccatatcgg cattttgggg attttcttcg ggcacttgtt cggcatgtta
     7921 acgccgcact ggatgtacgc gtggtttttg ccagtggcag cgaaacaact aatggcgatg
     7981 gtgctcggcg gtatttgcgg tgtgttgaca ttgattggcg gcgcaggact gctgtggcgc
     8041 aggctgacaa atcagcgcgt gcgtgcaact tccactacgc cggatattat catcatgagc
     8101 attttgctga tccagtgtct gctggggcta agtaccatac cgttttcagc acagtatcct
     8161 gatggtagcg aaatgatgaa gctggtgggt tgggcgcaaa gcattgtcac tttccgtggt
     8221 ggatcgtcag aaatgcttaa cggcgtagcg ttcgttttcc gcctgcatct ggtattggga
     8281 atgaccatct tcctgctctt cccgttcacc agattggtgc atgtgtggag cgcgccgttt
     8341 gagtacttta ctcgtcggta tcagattgtg cgttcgcggc gataatttca ccctggccgg
     8401 atagcccttc gctctccggc cacttgcagg ctatgatagt tctccagtca caggagaacg
     8461 ttaatgaaac cgcaggtgta ccacgtcgat gcttttacct ctcaaccatt tcgcggcaat
     8521 tctgccggtg ttgttttccc cgccgataat cttagcgaag cgcaaatgca gcttatcgcc
     8581 cgcgagttag gccattcgga aactgctttt ctgctgcaca gcgacgacag cgatgtgcgc
     8641 atccgctact ttacgccaac ggttgaagtg ccgatttgcg gtcacgcgac agtagctgcg
     8701 cactatgtac gtgcgaaggt gttaggttta ggaaattgca cgatctggca aacatcgctg
     8761 gcaggaaaac atcgcgtgac tatcgaaaag cacaacgatg attatcgtat ttcgctggaa
     8821 caaggtacgc cgggctttga gccaccgctg gaaggtgaaa cacgtgaggc gattatcaac
     8881 gcgcttcatc tcactgaaga cgatattttg ccgggcttgc caattcaggt cgcaacaaca
     8941 ggacactcaa aagtgatgat cccgttgaaa ccggaagtgg atatcgacgc cctttcgcct
     9001 gatcttgctg cgctgagcgc tatcagcaaa cagattggct gcaatggttt cttcccgttc
     9061 cagatccgcc caggcaaaaa cgaaaccgat ggtcgtatgt tctcgcctgc gattggtatt
     9121 gtggaagatc cggtgactgg caatgccaat ggcccgatgg gtgcatggtt ggtacatcac
     9181 aacgtattgc cccacgatgg caacgtgtta tgtgttaaag gccatcaggg gcgcgcattg
     9241 gggcgtgacg gcatgattga agtgacggtg acaattcgtg ataaccaacc ggaaaaagtc
     9301 accatttcag gcacagcggt gattttgttt catgccgaat gggcaattga actctgaaac
     9361 cgttgcctga tgcgcttcgc ctgtcaggtc tacaaccgag ctaatgtatg ccggataagg
     9421 cgtttacgcc gcatccggca acctgacata aattattttc ccgcctccgg gtgcgtatca
     9481 aacgccgcca tcaccagcgc taactcatcc acggtaaagc catgtttcgc atcatccacg
     9541 cccagaccaa actgttcttg catcacagca tataatgacg ccacatccgg tagatttcgc
     9601 tgctccaccg cgtgcccatt ttcataatgg gtaaaatgaa aattggtcag cgttagcttg
     9661 ccaccgtccg gcaaatggcg gcacatcagc aaatgatggc gaaaatgcga ctgcggccag
     9721 tgcgccgacc agaaattgcc catcacataa tcgctttgtt gctgctcgca gagatcaaaa
     9781 cggtacatcg actgccagtg ctgatgatga ttaaactgca acacccaatc atcaccctcc
     9841 tgcaacaacc gataatctcc gtgtggcgtg gtctgcacga gatcggaaac taaacgaatc
     9901 ggcgcggtta gcgtctgccc accgaaaccg acatcagcaa tccatttttc ctcttccagt
     9961 tccaccaaca gcaaacgatg ggtgcgcggc ggtaatgctg gcggatttga taacactacg
    10021 cgccctaaca agctgcgaac gttaaactcc agctcgcgta acacccgctc aaacacgcca
    10081 ttctgctcaa aacagtaacc gccacgacgg gctatcacca gtttctcttc cagcgattga
    10141 tcatcaagct gtatttccct cggcagcaaa acgtcgaggt tttcaaacgg aatagtgcaa
    10201 ttgtgtttca ggtgcaatgc acgaagcgta tcaatattga ccgcagcagc tcccgaccag
    10261 ttaatacggg caaaatagtg attcagaatg ggcgtcatca ggcgtttccc ttagtggttt
    10321 tctcaactta taacctggaa tagacgccgc accggctatt ttgtgcgatc gcggacatgc
    10381 atcacgtttg caaatcgcca atcccgttaa tttcgttact tattgcgtta cggactacgc
    10441 ttaaaaaaac cttcttcgcg aggcgatacc atgagccgat tcatccccat agaattacac
    10501 catgccagcc gtctgttaaa tcatggccca actgtcatga tcaccagttt cgatgagcaa
    10561 tcccagcggc gtaacattat ggcagcagcc tggtcaatgc cggtggagtt tgaaccgcca
    10621 cgcgtggcga ttgtggtaga taaatcgaca tggaccaggg agttgattga acgtaacggt
    10681 aaatttggca tcgttatccc gggcgttgca gcaactaact ggacgtgggc agtgggaagc
    10741 gtgtcgggtc gtgaagaaga taaatttaat tgctatggca ttccggtggt gaaaggtccg
    10801 gtgcttggtt tgccacttgt tgaagagaaa tgcctggcgt ggatggagtg ccgcctgcta
    10861 cctgcgactt ctgcgcaaca aaaatacgac acgctgtttg gtgaggtggt atcagcagcg
    10921 gcagacgcac gggtatttgt cgaaggccgc tggcagtttg atgatgataa gctcaatacg
    10981 ttgcatcatt taggtgctgg cacgtttgtt accagcggca agcgagttac ggcgggttaa
    11041 gcattacatg ctgtaaccgg gtttctttat caaagcctcc atttggggcg cgatttcggc
    11101 atcccagata gcttgccaag attctggttg aatctgctgt agagcaatgc ttatcgagct
    11161 atctttactg ttcagatggc gaataataac gtcggtaata tctgcagcaa gtgctgcttt
    11221 ttattgttcg tccagttcac gcggaaaaca tttaatgtcg atgtgcggca tgattgtgct
    11281 ccttttgaaa gatgtcagta aaattaacac acattgtgaa tgtcgtgtgt ccggtaatgc
    11341 gtttttacga caataatgcc agggcaagat tacgaaagcc ttctccccgc aaggactgac
    11401 gccaggtagt ttctgtccat ggctgctttt cgcatcttac gtcttaaccc tgccttgaat
    11461 accttatcat tcgtcaaaat attaatagca atgtgccgta tccctgaaaa taattctgct
    11521 gcatttcctt ttcttatttt gcagtcgtct tcattcatta ccacgtccag acgccagtgc
    11581 agcttattct ccacgtgcca
//
LOCUS       DAZGJW010000139        11529 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZGJW010000139 DAZGJW010000000
VERSION     DAZGJW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.139"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            130..747
                     /gene="yijF"
                     /locus_tag="ACHKIK_003957"
     CDS             130..747
                     /gene="yijF"
                     /locus_tag="ACHKIK_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO9085035.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(774..1679)
                     /gene="yijE"
                     /locus_tag="ACHKIK_003958"
     CDS             complement(774..1679)
                     /gene="yijE"
                     /locus_tag="ACHKIK_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO9085036.1"
                     /translation="MSVAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(1773..3953)
                     /gene="katG"
                     /locus_tag="ACHKIK_003959"
     CDS             complement(1773..3953)
                     /gene="katG"
                     /locus_tag="ACHKIK_003959"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO9085037.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(4282..5172)
                     /gene="metF"
                     /locus_tag="ACHKIK_003960"
     CDS             complement(4282..5172)
                     /gene="metF"
                     /locus_tag="ACHKIK_003960"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO9085038.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            5248..5382
                     /locus_tag="ACHKIK_003961"
     CDS             5248..5382
                     /locus_tag="ACHKIK_003961"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085039.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(5521..7953)
                     /gene="metL"
                     /locus_tag="ACHKIK_003962"
     CDS             complement(5521..7953)
                     /gene="metL"
                     /locus_tag="ACHKIK_003962"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO9085040.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPVSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(7956..9116)
                     /gene="metB"
                     /locus_tag="ACHKIK_003963"
     CDS             complement(7956..9116)
                     /gene="metB"
                     /locus_tag="ACHKIK_003963"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO9085041.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVMTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            9393..9710
                     /gene="metJ"
                     /locus_tag="ACHKIK_003964"
     CDS             9393..9710
                     /gene="metJ"
                     /locus_tag="ACHKIK_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO9085042.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            9894..10502
                     /gene="yiiX"
                     /locus_tag="ACHKIK_003965"
     CDS             9894..10502
                     /gene="yiiX"
                     /locus_tag="ACHKIK_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO9085043.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYIFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            10647..11322
                     /locus_tag="ACHKIK_003966"
                     /pseudo
     CDS             10647..11322
                     /locus_tag="ACHKIK_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(11409..>11529)
                     /locus_tag="ACHKIK_003967"
     CDS             complement(11409..>11529)
                     /locus_tag="ACHKIK_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFO9085044.1"
                     /translation="YESPTSTHNDIMGGGGMSYAYEYDVKYNICHLRKKSRMR"
BASE COUNT         2824 a         3210 c         2893 g         2602 t
ORIGIN      
        1 tctgtgccag taatctgaaa ctcctacgtt gttcaggagt tttttatccg cttctggaca
       61 ttttctctac agaacaatcg ttagcccgga aggtcgaatc tgtgactctt attttcacgg
      121 acgaaccaga tgaaagcttc actggcgctg ctcagcctgc taacggcatt taccagccac
      181 tccttaaaat ctcccgccgt tccaccaacc gtggtacaaa tccaggccaa taccaacctt
      241 gccatcgcag acggtgccag acagcaaatt ggcagcacgc tattttacga tccggcgtat
      301 atgcagctta cctatcctgg cggtgatgtc ccgcaagaac gcggggtatg ttccgatgtg
      361 gtgatccgtg cattgcgcag ccagaaggtc gatttgcaga aactggtgca cgaagatatg
      421 gcgaagaatt ttgccgagta cccgcaaaaa tggcagttaa agcgcccgga cagcaacatc
      481 gatcaccgtc gggtgcctaa tctggaaacc tggtttaccc gccacgataa aacacgcccc
      541 atcagcaaga accccagtga ctatcaagcg ggcgatattg tctcctggcg actggataac
      601 ggactggcac atattggggt ggtgtcagat ggcttcgccc gcgacggtac gccgctggtg
      661 atacataaca ttggcgcagg cgcgcaggaa gaagatgtgc tgtttagctg gcggatggtg
      721 gggcattatc gttattttgt taaataagcg atcgccccac atgaagaaaa aattcagatg
      781 ctttttacac tgacagcttc ttttttctta cggctcacca gcgccagtgc cagcacaatc
      841 agcacaatac cgctaccttc aacggccccc ggattctcac cgagcagcca ccaggaaaag
      901 agtacgccgc aaacgggaac ggccagcgtg cttaagctgg caatactggc aggcaagttt
      961 ttcaatacaa acaaccataa gctccacgcc agcgccgtcg ccagaatcgc actgtaggcc
     1021 agcgcccaga acacggtggg ctgccagtca atttcacgtt gcggcaccag taaagcgacc
     1081 acactcatca ccagcgccgc atagagcatc tgccaggatg ttaacgacaa taaatccacg
     1141 cgcggatgac gggcatacag acgtttagca acaatcgcgc tcgcccccca actgacgccg
     1201 gagaggattg ccagcatggc acttttcatc gaagagaaat cgagttgcca cggctgcaac
     1261 accaaaaata agccgaaagc ggcaatcagt atcgcgaaat attgcccacg tcgcaggcgt
     1321 tcaccgagaa acaacgcggc gaaaatcacc acccagaacg gcatggtata gcttaggatc
     1381 gccaccttcc ccgctccgcc gctgaccagc gcccactgcg ccaggccaac catcccgcag
     1441 gtttgtaaca gggcaatggc taaggtgtat ttaaacggtg tcgggcgcat tccgcgacca
     1501 cgtaataaaa ggacgatgaa taaaacgaga gcgccgaaaa tgcagcgtaa ggcggtaaag
     1561 tcgaaggcac cgatgtagct ggtgacttgc ttcatgaaaa tccagctata actccagata
     1621 agtgtgagca caaccaggcc actgattgcc agtgggttgc tctttcctgc gacagacatg
     1681 agagatccag tgtgtagttt tcctgtggac actatacgcc ggtaaagaaa cttcagcgac
     1741 tgcctgaaag gcagtcgctg aagaaggtca gattacagca ggtcgaaacg gtcgaggttc
     1801 atcactttca cccatgccgc cacgaagtct ttaacaaact tctcgtgagc atcgctgctg
     1861 gcgtaaactt cagccaccgc acgcaggaca gagttagaac caaataccag atccgcacgg
     1921 ctggcagtgt atttcacttc gccggtttcg cggtcacggc cttcaaacag ctctttcgat
     1981 tcgtcggtcg ctttccactc gtaacgcata tccagcaagt tcacgaagaa gtcattgctc
     2041 aatacgccaa cgcggtcagt gaagacgccg tttttgctgc catcgaagtt ggcacccagt
     2101 acacgcatcc cgcccaccag cgcggtcatt tccggcgcgg tcagggtcag ttgctgggct
     2161 ttgtcgatca gcagtgactc ggtggtggaa acatccagac gagcgcgata gttacggaaa
     2221 ccgtcagcaa ttggctccag cagctcgaac atctcaatgt cagtctgatc ctgacgcgca
     2281 tcaacgcggc ccggcgcaaa cggtacatga atgctcaaac ctgcggcgct tgcggctttc
     2341 tcaacaccaa ccacaccagc cagcacgatg atatccgcca gcgaggcttt accagactct
     2401 ttctggattt tctccaaaac aggcagagca cgaacggctg cggcgttcac atcccagtcg
     2461 cgctgcggca ttaatgccag acgcgcaccg ttggcaccac cgcgtttgtc gccaccacgg
     2521 aaggtagaag cagaagccca ggccaccgat accagctcac taacagacag accagaatcc
     2581 gcaatcgcga atttcagatc gataatgtcc tgctcggtcg ggttgtagat cggctgcggc
     2641 agcggatctt gccagatcag atcttctttc ggcacttccg gcccgatgta gcgagatttc
     2701 ggccccatat ccctgtgcgt cagtttgaac caggcacggg caaaggcttc gttgaacgcc
     2761 tgcggatcgt tgaggaaacg acgagagatc ttctcgaact caggatcaaa acgcagcgtc
     2821 aggtcggtca ccagcatcgt cggtttacgt ttcttcgacg gatcaaacgg atccgggata
     2881 atttccggtg cgtctaccgc ttcgaactgg attgcgccag ccgggctgcg ggtctgtacc
     2941 cactcatact tgaacaggtt ctcgaagaaa tagttgctcc actgggtcgg cgtctgggtc
     3001 cagactactt ccagaccaga ggtaatggca tctgcgccaa cgccgctgcc gtaagtgctc
     3061 gcccaaccta aaccttgttc ttcaatcggt gcagcttctg gatcaggacc tacatttgat
     3121 gtcggaccgg caccgtgggt tttacccagc gtatgaccac ccgcaataag cgccacggtt
     3181 tcttcgtcgt tcatgcccat gttgccgaag gtcgcacgga tagctgctgc cgcagaaagc
     3241 ggttcgccgc tgtgatccgg gccttccggg ttaacgtaaa tcaggcccat ctcggttgca
     3301 cccagcggtg ctttcgccag cgcttccgga tgacggtggg tcagccaggc tttttcatca
     3361 ccccagttaa catccagatc cggttcccag acgtcttcac gaccggcacc aaaaccgaag
     3421 gtacggaagc cggagttttc cagcgccacg ttacccgcga ggataaacag gtcggcccag
     3481 gagattttct gaccatattt ctgtttgatt ggccacaaca ggcgacgcgc tttatcgagg
     3541 cttacgttat ccggccagga gttcagcggt gcaaaacgtt gctgaccacg acccgcgccg
     3601 ccgcgtccat cgattgaacg gtaagtcccc gcgccgtgcc aggccatacg aataaacaga
     3661 ccggcgtagc tgccccagtc ggcaggccac cacggctgag attctgtcag cagggctttc
     3721 agatcttttt tcaggccgta gtaatctaat ttgctgaatt ctttgcggta gtcaaagtcc
     3781 tcacccagcg ggttagaacg attagaatgt tggtttaaca ggtcaacacg gagttgattt
     3841 ggccaccagt cgcgagtggt tgtgcccgcc cccgcgctct ggtcgtgacc gccctgatgg
     3901 aacgggcatt tgccagtggc tgtcgtgtta tgaatatcgt ctgacgtgct catcaatgtg
     3961 ctcccctcta cagtgttacc gttacgatac acagcgttag agagaagtta taattgaatt
     4021 aatccacgga tgcgatagtt gagatcttac atattgttgg ttaaagagat gtagatcaaa
     4081 ttgatcgtaa ttaagctttt ttgattatgt ccgaattcgg acaactgctt tataaaaaag
     4141 ggttttagat tcctattctt caaggaagga gggcgaaaac aaagctgaca accttatttt
     4201 tacgccctca tttcgaggca gcattttgtg ctctgtttaa aatttgtgat cacagtgtga
     4261 ttttcacaaa agccacacta tttataaacc aggtcgaaca cccagcgtat ggcaaatcgc
     4321 gtaactcatt tcagcgcggt taagcgtata gaagtggaaa tctttcactc cttcacggct
     4381 taaaatcttc accatatcca tggcaatatt cgcgcccacc agcttgcgcg tttcagcatc
     4441 gtcatccagc ccgtcaaaca tttgtgccat ccacgccgga atacgcacgt tggtcatatc
     4501 ggcaaatttc ttcgcctgtt taaagttaga taccggcaaa atccccggaa taatttccac
     4561 atcaatgccc gccgatacgc agcggtcacg aaaacgcagg tagctttcga catcaaagaa
     4621 gaactgagta atcgcgcggt tggctccggc atcgactttg cgtttcaggt tgagcaaatc
     4681 cgcctgagcg ctttttgctt ccgggtgaac ttccggatac gccgccacgg agatatcgaa
     4741 atctgccact tcttttaaca gcgtcaccag gtcagaagca tacatttccg gcttaccact
     4801 tcccggcggc agatcgccac gcagcgccac gatatgacga ataccgttat tccagtagtc
     4861 gcgtgcaatg gtgcgcagct cgtcgggcgt cgcatcaatg caagtaagat gcggtgccgc
     4921 ttccagacca gtgcgatctt taatgccttt aataatgctg tgcgtacggt cgcgctcgcc
     4981 ggagttcgcg ccataggtca ccgatacaaa cttcggtttc aggctgctaa ggcgatcgat
     5041 ggagttccac agggtctgct ccatttcact ggtacgcggc gggaaaaact cgaacgaaac
     5101 gttaatctgc ccctggactt ctgccaggct ctgattcagg gcatcccgct ggctggcgtg
     5161 aaaaaagctc ataccttacc tcatcaatcg cttgtagtta tatgttgtgt tgtgcacatc
     5221 tatccattta gacgtccaga tgtaaagatg aaggaaaagc cgaagggcgt caaccgaaaa
     5281 atgaccaatg atggtgagga aaaatcaggg aagatgaaaa aacttcagga tggtaagaaa
     5341 aagaaaatcc ggtatgcaaa gcaaaccgga ctgcaaatat aaactaacca tgtacttatt
     5401 gcttcttgcc ggattcgtcg taaacgcttc atccggccta atcctatgat aattcaatat
     5461 atttcattag aaaccataga cccgaaaaca tgagtaccgg gcattattaa atttctgaaa
     5521 ttacaacaac tgtgccagcc ggttgatatc cgactgaatc gccccggcgg tgacgtcgcg
     5581 cccagcgcca ggcccacgga tcaccagcgg attatcgcgg taccagcggc tttcgatggc
     5641 aaagacgtta tcgcatggca gcagtgatgc cagcggatgg tcttcacgca ccgcttccac
     5701 gcccacacgc gctttgccat tggcatcgaa acgcgccacg tagcgcagca ccagccccat
     5761 ttcgcgggcc gcttccagcc gttgcaccat ctgctcgtta agttcatcgc cattttcaaa
     5821 gaaatggtcg atgctgccgc cttcgcaatg agcaggcacc agcgattcca cacgtacctg
     5881 gtccggttca atgttgtaac ccgcttcacg cgccagaatt accagcttgc gcattacgtc
     5941 tttgccggag aggtcgtcac gcggatcagg ctcggttaag ccctgctgcc acgcctgatc
     6001 caccagctcg gtaaacggca cgctaccgtc gaattgcagg aacagccagg agagcgtgcc
     6061 ggagaagatc ccgctgatcg acaaaatagt atcgccgctg tcgatcagat cgcgcacggt
     6121 gtggttgatc ggcaagcccg caccgacggt ggcattgtac agccagtgac gcccggtttt
     6181 ttcgaaggcg tcgtggatct ggcgatattt attgctgtcg ctggctcccg ccagtttgtt
     6241 ggcgctgata acgtggaaac cgtggctggc gaaatcaaga tactgatcag caagctgctg
     6301 gctggcggta acgtccagca ccactaaatc atcatacgga tgggcgcgca tccacaggaa
     6361 caacgactct tcatcctgct caaccgcttc atcgttgaag aaggctaacg cgcggctggc
     6421 gtccagcccg tcatagctca acaggctgcg gcggctgtcc accacacctg ccagcacaaa
     6481 ctcaaagccg gtacgtgccg aaagcgtgct ctgctcacgg gcgaacagtt ccagccaacg
     6541 ggaaccgata ttgcccttac cgaacaatac caggccgatg cgtttttctg cgcggaagac
     6601 ggactgatgc agcccctgaa tcaggctttc ggtcgggccg gtgcgcagta ctgccaccag
     6661 gctgatgccg tcatcggact gccaggtaaa ttcgaccggc tggcctttca gttgctgcca
     6721 gaagcggtgg caatgcagcg ggttacgggt gacgcctgca ccgaccatcg ccaccagcgc
     6781 cagcccctga cgcaggcgca gttcgccagg taatcccgct tcgtcgagga ttttcagcgc
     6841 actgtcggcc acttctgagg tgtagcaaaa ttgcagcaac tggcgatcgt tatgtacgcc
     6901 aaccgccagc gggcgtacct gcgcgcgttt caggatttgg tcgatctctt tatgcgccag
     6961 tttgaaatcc tgactgacgg gcacctgaaa ctcaatcaaa cagacatcat cgtggctggt
     7021 gacaatacgc gcaccagtac cggaggccag cacgcgttca atgcgcgtgg aaccttgatc
     7081 cggcgtgtag ctacagcgca gttgcaggtc gatttcgctg ccagaaaccg gctgtaaagt
     7141 acgggcgtga agaacgggag ccgccaggcg cgccagttcg ctggcctcat ccagacgcag
     7201 caacggcagc aggcaggcat ctttcacttt acgcgggtcg gcactgtata ccccggcgac
     7261 gtcgctccag atggttacgc gagaaacacc cgccagcgca ccgatttgtg tcgcggaata
     7321 gtcggaaccg ttacgcccca gcagcaccgt ttcaccggcg ttgttgcggc tgataaatcc
     7381 ggtcaccacc agacgtttgc ccggatgttg caccagcagc tgttgcagca acgggtaaga
     7441 aagcccttca tcaacctgcg gttgtgcggc gcgttcagcg cgtaaaaact cgcgggcatc
     7501 aagccaggcc gctggcagcc cttgttgatt aagtaccgca gacatcaggc gtgccgacca
     7561 tacttccccg tggcccacca cttccgcata cactgcgtcg ttaataccgc tgtcgagcag
     7621 cgccgccagg cgctcaaggt cgctgacaaa agcgctaatg aggctatcgg cttcttcagc
     7681 gggtagcaga ccgctaatca gatcgcactg atagcgacgc agcgtttgtt gaacctgatg
     7741 cgcagagaga cgatcggtct ggcttagttt caaccagtta atcaactggt tagtggtgct
     7801 accggcggcg gaaaccacca tcatatcgtc aggctgagag tactccgcca taatgcccgc
     7861 gacacgcaaa taacacttca catcagccag actactgcca ccaaatttat gcagctgacg
     7921 acctttcgcc cctgcctgcg caatcacact catttttacc ccttgtttgc agcccggaag
     7981 ccattttcca agtcggcaat taaatcttcg ccatcttcaa taccggtgga gatacgcagc
     8041 agcgtctcgg aaatcccggc ggcagcacgc gcttctggtg ccatgcctgc atgtgtcatg
     8101 gttgcggcgt gagagattaa actttccact ccccctaatg attccgccag cgtaaacaac
     8161 gacagcccgc ccaggaaacg acgcagcgtc tgctcatcgc catccagttc aaaactcaac
     8221 attgcgccaa agcctttttg ctgacgcgcg gcaatttcat gcccctgatt ttccggcagc
     8281 gacgggtgat acagtttttt caccaacggc tgggtttgca gatatttcac aatcgcctgc
     8341 gcgttgcgct gcgccagctc catacgcggc accagcgttc gcaacccgcg tagcagcaga
     8401 tagctgtcaa acgcgccgcc cgtcacgcca atattgtttg cccaccaggc aagttcagtg
     8461 acaacgtccg ggtctttagc aatcaccacg ccagccacca cgtcagagtg accgttcaga
     8521 tatttcgtac atgaatgcaa caccagatcg gcacccaatg ccagcggatt ttgtaatgcc
     8581 gggcttaaga aggtgttatc caccacgctc accgccccga cttcccttgc cagatggcag
     8641 attttcgcaa tatccacgac gcgtaacaat ggattacttg ggctttctac cagtaccagt
     8701 ttgggttttt ctgccagtgc tgcccgtaat gcctgttcat cgccttgatc aacaaacaac
     8761 acgcgatagc aaccgcgttt cgccaggctg tcgaacaggc gatagctacc gccgtagcag
     8821 tcgtgcggcg caaccagcag atcgccaggt ttcaaaaaga cggtcattac caggtgaatc
     8881 gcggacatgc cggtattagt aagtactgca ccagcaccgc cttccagttc tgccagcgca
     8941 cgctgaacca catcgcgcgt tgggttgccg cgacgcgagt aatcatgcgc gcgcggttca
     9001 ttaaatccgg taaagttata tgtgctggaa agatggatcg gtgggacaac gcaaccatac
     9061 tgttcgtcgt catttaaccc gctacgcact gcgatggtgg cctgtttacg cgtcatgtga
     9121 tgaagttccc tgggctttgt cggtgaaatg tcaggcacca gagtaaacat tgtgttaatg
     9181 gacgtcaata catctggaca tctaaacttc tttgcgtata gattgagcaa atcccaaata
     9241 gccgttaaaa ttatatgcat tatcacgccg acaggtgcat tacacgatgt cacggtaacg
     9301 cctgtacggt aaactatgcg ggtttacggt cagtacccac ataaattgtg tggtctggtc
     9361 tcaatttatt gacgaagagg attaagtatc tcatggctga atggagcggc gaatatatca
     9421 gcccatacgc tgagcacggc aagaagagtg aacaagtcaa aaagattacg gtttccattc
     9481 ctcttaaggt gttaaaaatc ctcaccgatg aacgcacgcg tcgtcaggtg aacaacctgc
     9541 gtcacgctac caacagcgag ctgctgtgcg aagcgtttct gcatgccttt accgggcaac
     9601 ctttgccgga tgatgccgat ctgcgtaaag agcgcagcga cgaaatcccg gaagcggcaa
     9661 aagagatcat gcgtgagatg gggattaacc cggagacgtg ggaatactaa agcggaaatg
     9721 agcggcgcgg agtggaatcg cctgatgcgc tacgcttatc aggcctacgt catattgcaa
     9781 tttattgaat ttgcacgaac ttgtaggccg gataaggcgt tcacgccgca tccggcataa
     9841 acaacgagca cgttgtctgc gacccaccgc tttttataca tggacgttta actatgaaaa
     9901 acaggctgct gatcctcagc ctgctggttt ctgtacctgc ctttgcctgg cagccgcaaa
     9961 ctggcgacat catctttcaa atctctcgct catcgcaaag taaagcgatc caactggcaa
    10021 cccattccga ttacagccac accggtatgc tggtgatgcg taacaaaaag ccctacattt
    10081 ttgaagcggt cggcccggtg aaatacaccc cgctcaagca gtggattgcc catggtgaaa
    10141 agggcaaata cgtcgttcgc cgcgttgaag gcggactgag tgttgaacaa cagcaaaaac
    10201 tggcgcaaac ggcaaaacgt tatctcggta aaccgtatga tttcagcttc tcatggagcg
    10261 acgatcgcca gtactgttcg gaagtggtgt ggaaggttta ccagaacgcg ctgggaatgc
    10321 gcgtgggcga acagcagaag ttgaaagagt ttgatctcag caacccgctg gtccaggcga
    10381 agctcaaaga acgctacggt aaaaatattc cgcttgagga aacggtagtt tcaccacaag
    10441 ccgttttcga cgcgccacaa ctcaccacgg ttgccaaaga gtggccgctg ttttcgtggt
    10501 aatggatgat gaaaaaaata attcccgccg tagtctgttt gttgctttgt agttgcacga
    10561 cctttccgca agctgtcgct ccggtaaacg gtgatgcctc caattagttg aattaaggat
    10621 ataatactcg cttttaaggt tgtttcatgg tcacagttaa ccttcattgc ccccgttgcc
    10681 agtccgttca ggtttaccgt cacgggcaga acccaaaaag acatgatcga ttccgcggcc
    10741 gtgactgtca ccgggtattt cagctcagtt actgttacga gccccgtaaa ccgggagtta
    10801 aagaccaaat cgctgaaatg actttcaacg gtgccggggt tcgcgatact gcccgtacgc
    10861 tgaaaatcgg cattaataca gtcatccgaa ctttaaaaaa ctctcgccac gaagaataac
    10921 gtcatccccg gtcgctcatg ctgatgtggc actcatctgc aaacttagtc agcaatggag
    10981 ctttgtcggc agtaaagcgc gacagcactg gctctggtac gtgtacaaca cgaaaactgg
    11041 tggtgttctg gcctacactt ttggcccccg gactgacgaa acctgtcgtg aattgcgggc
    11101 gctgcttacc cttttaccat cggcatgttg accactgacg aatggggtag ttacgctaga
    11161 gagttaccga aggagaagca tctgactggt aagattttta ctcagcacat tgagcgtaac
    11221 aatctgaccc tgagaacccg aatcaaacgt ctggcccgca gaaccatctg tttctcgcgt
    11281 tctgtagaac ttcatgaaaa agtcatcggt gcttttatcg aaaatatatg ttctactaat
    11341 tggagtcatc acccttctga taagctttcc ttggatgatt accagactga atacacagcc
    11401 aaagattttt acctcattct gctctttttt ctcaaatgac atatattata ttttacatca
    11461 tattcatagg catatgacat tcccccgccc cccattatgt cattgtgggt tgatgttggg
    11521 ctttcataa
//
LOCUS       DAZGJW010000140        11406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZGJW010000140 DAZGJW010000000
VERSION     DAZGJW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.140"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..421
                     /locus_tag="ACHKIK_003968"
     CDS             <1..421
                     /locus_tag="ACHKIK_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO9085045.1"
                     /translation="GLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLN
                     DDQRRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRA
                     LAAELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            436..1293
                     /gene="menB"
                     /locus_tag="ACHKIK_003969"
     CDS             436..1293
                     /gene="menB"
                     /locus_tag="ACHKIK_003969"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO9085046.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1293..2255
                     /gene="menC"
                     /locus_tag="ACHKIK_003970"
     CDS             1293..2255
                     /gene="menC"
                     /locus_tag="ACHKIK_003970"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO9085047.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            2252..3607
                     /gene="menE"
                     /locus_tag="ACHKIK_003971"
     CDS             2252..3607
                     /gene="menE"
                     /locus_tag="ACHKIK_003971"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO9085048.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            3717..3983
                     /gene="pmrD"
                     /locus_tag="ACHKIK_003972"
     CDS             3717..3983
                     /gene="pmrD"
                     /locus_tag="ACHKIK_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO9085049.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(3977..4363)
                     /gene="arnF"
                     /locus_tag="ACHKIK_003973"
     CDS             complement(3977..4363)
                     /gene="arnF"
                     /locus_tag="ACHKIK_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO9085050.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(4363..4698)
                     /gene="arnE"
                     /locus_tag="ACHKIK_003974"
     CDS             complement(4363..4698)
                     /gene="arnE"
                     /locus_tag="ACHKIK_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO9085051.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4695..6347)
                     /gene="arnT"
                     /locus_tag="ACHKIK_003975"
     CDS             complement(4695..6347)
                     /gene="arnT"
                     /locus_tag="ACHKIK_003975"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO9085052.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(6347..7237)
                     /gene="arnD"
                     /locus_tag="ACHKIK_003976"
     CDS             complement(6347..7237)
                     /gene="arnD"
                     /locus_tag="ACHKIK_003976"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO9085053.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7234..9216)
                     /gene="arnA"
                     /locus_tag="ACHKIK_003977"
     CDS             complement(7234..9216)
                     /gene="arnA"
                     /locus_tag="ACHKIK_003977"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO9085054.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9216..10184)
                     /gene="arnC"
                     /locus_tag="ACHKIK_003978"
     CDS             complement(9216..10184)
                     /gene="arnC"
                     /locus_tag="ACHKIK_003978"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO9085055.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10188..11327)
                     /gene="arnB"
                     /locus_tag="ACHKIK_003979"
     CDS             complement(10188..11327)
                     /gene="arnB"
                     /locus_tag="ACHKIK_003979"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO9085056.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTPMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTTIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
BASE COUNT         2779 a         2958 c         3056 g         2613 t
ORIGIN      
        1 ggggctgcaa aatgctgaac aacgtgcgga acgtcagcgt tccgatcgcc aatgggcgca
       61 gcgtttttgc acagaaccgt taacggcggt atttgccgac tggtatcaac agcctgtttt
      121 tgcctcgctc aatgacgatc aacgccggga gctggtggcg ctgcgcagca acaataatgg
      181 cgcaaccctt gccgccatgc tggaggcgac ttctctcgcc gtccagcctg atttacgtgc
      241 taaccttagc gcccgcacat ttgcgtttta ttatttatgt ggtgaacgtg acagcaaatt
      301 ccgcgccctg gcggcggaac tggctgccga ctgccatgtc attcctcgcg ccggacataa
      361 cgcgcatcgg gaaaatcccg ctggcgtaat cgcaagtctg gcgcagatct tgcgtttctg
      421 actaaaggac acaatatgat ttatcctgat gaagcaatgc tttacgcacc ggttgaatgg
      481 cacgactgct ccgaaggttt cgaggacatt cgttatgaaa aatccaccga cggtatcgca
      541 aaaatcacca ttaatcgtcc gcaggtgcgc aatgccttcc gtcctctgac ggtaaaagag
      601 atgatccagg cactggcaga tgcgcgttat gacgacaata tcggcgtgat cattctgact
      661 ggcgcaggcg ataaagcgtt ctgctccggt ggtgaccaga aagtgcgtgg tgattacggc
      721 ggctataaag atgattccgg cgtacatcac ttgaacgtgc tggacttcca gcgtcagatc
      781 cgtacctgtc cgaaaccggt tgtcgcgatg gtggctggct actccatcgg cggtggtcac
      841 gttctgcaca tgatgtgcga cctgactatc gcagcagata atgccatctt cggtcagact
      901 ggcccgaaag tcggttcctt cgacggcggc tggggcgctt cctacatggc tcgtatcgtc
      961 gggcagaaaa aagcgcgaga aatctggttc ctgtgccgtc agtacgacgc aaaacaggcg
     1021 ctggatatgg gccttgtcaa caccgtggta ccgctggcgg atctggaaaa agaaaccgtc
     1081 cgttggtgcc gcgaaatgct gcaaaacagc ccgatggcgc tgcgctgcct gaaagctgcg
     1141 ctgaacgccg actgtgacgg gcaggcgggg ctgcaggagc tggcgggcaa cgccaccatg
     1201 ctgttctaca tgacggaaga aggtcaggaa ggtcgcaacg ctttcaacca gaaacgtcag
     1261 cctgatttca gcaaatttaa acggaatccg taatgcgtag cgcgcaggta taccgctggc
     1321 agatccccat ggacgcgggg gtggttctgc gcgacaggcg gttaaaaacc cgcgacgggc
     1381 tgtacgtttg cctgcgtgag ggcgagcgcg aagggtgggg ggagatctcc ccactgccgg
     1441 gcttcagcca ggaaacctgg gaagaggcgc aaagcgtgct gcttgcctgg gtaaataact
     1501 ggctggcagg cgattgcgag ctaccgcaga tgccttccgt tgcctttggc gtaagctgtg
     1561 cattggcgga gctggcagat acgctcccgc aggcggccaa ttaccgtgcg gcaccactgt
     1621 gtaatggcga tcctgacgat ctgatcctca aacttgctga tatgccaggc gagaaagtgg
     1681 cgaaggtcaa agtgggattg tacgaagccg tgcgcgacgg catggtggtt aatctgttgc
     1741 tggaggcaat tccggatctg catttgcgtc ttgacgcaaa tcgcgcctgg acaccgctga
     1801 aaggccagca gtttgccaaa tatgttaacc cggattatcg ccaccgcatc gcgtttctcg
     1861 aagagccgtg caaaacccgc gatgattcgc gagcgtttgc ccgtgaaacc ggcattgcca
     1921 ttgcctggga tgaaagcttg cgcgagccgg attttgcctt tgtggctgaa gagggcgtgc
     1981 gcgcggtagt tatcaaaccc acgctcacgg gcagtctgga gaaagtacgc gagcaggtac
     2041 aggcggcgca cgcgctgggg ctgacggcgg tgatcagttc ttccattgaa tcgagcttag
     2101 gcttaacgca actggcgcgg attgccgcct ggttaacgcc ggacaccatt ccagggctgg
     2161 acacgctgga tctgatgcag gcgcagcagg tacgtcgctg gccgggtagc acgctgcctg
     2221 tcgtggaagt tgatgcactg gagcggttgt tatgatcttc tctgactggc cgtggcgtca
     2281 ctggcggcaa gtgcggggag aagccatcgc cttacgtctt aatgacgagc aacttaactg
     2341 gcgcgagctt tgtgctcgcg tcgatgaatt agcctcaggc tttgcggcgc agggcgttgt
     2401 tgagggcagc ggcgtgatgt tgcgggcgtg gaatacgccg caaacgctgc tcgcctggct
     2461 ggcgttactg caatgcgggg cgcgggtgtt gcccgtgaac cctcagctgc cgcaaccgtt
     2521 gcttgaagaa ttgctgccca atctgacgct gcaatttgcg ctggtgccgg atggggaaaa
     2581 cacgtttccg gcgttaacgt cgctgcacat tcagcgggtt gaaggcgcac atgccgctac
     2641 gtggcagccg acgcgtctgt gctcaatgac gttgacctcg ggttctaccg ggttgccgaa
     2701 agccgctgtc catacttatc aggcccatct tgccagtgcc gaaggggtgt tatcgttgat
     2761 tccgtttggc gatcacgatg actggttgct ctctttaccg ctgtttcacg tctccggtca
     2821 gggaattatg tggcgctggt tatacgctgg tgcgcggatg acggtgcgtg ataaacagcc
     2881 attggagcaa atgctggcag gctgtactca tgcttcactg gtgccaacac aactctggcg
     2941 tttgctggtt aaccgcagtt ccgtttccct gaaagcggtg ttacttggcg gcgcggctat
     3001 cccggtcgag ttgacggaac aggcgcgcga gcaggggatt cgttgctttt gcggctatgg
     3061 tctgaccgag tttgcctcca cggtgtgtgc gaaagaagcc gacggcctgg cagacgttgg
     3121 ttcgccgctg ccgggtcggg aagtgaaaat cgttaataat gaagtgtggc tgcgggctgc
     3181 cagtatggca gaaggttact ggcgtaacgg gcaactggtt tcactggtta atgacgaagg
     3241 ctggtacgct acgcgcgatc gcggtgagat gcataatggc aagctgacca ttgtcggacg
     3301 tttagacaat ctattcttca gtggcggaga gggtattcag ccggaagaag tcgagcgtgt
     3361 tattgctgca catcctgcgg ttttgcaggt gtttatcgtt cccgttgccg acaaggagtt
     3421 tggtcatcgg ccggtagcgg tggtggagta tgaccagcaa accgttgatc ttggcgaatg
     3481 ggtgaaagat aagctggctc gttttcaaca accggtgcgc tggctgactt taccgccgga
     3541 gctgaaaaac ggcggtatta aaatttcacg tcaggcgcta aaagagtggg tgcaacgtca
     3601 gcaataatta accctccgtt tcacttctca tttctgccat tccattgccg ttgataaaga
     3661 gtttatctgt taatctgtaa attgatgtga aaactcttag caaacaggat aatgcaatgg
     3721 aatggctggt caaaaaatcg tgctgcaata aacaagataa cagacatgta cttatgctct
     3781 gcgatgctgg cggcgcgata aaaatgatcg ccgaagtgaa gagtgacttc gccgtgaaag
     3841 tgggggattt actctcgcct ttgcagaatg cgctttattg tattaatcgt gaaaagctgc
     3901 atacggtgaa agtcctctcg gcaagcagtt atagccccga tgagtgggaa cggcagtgta
     3961 cagcggcagg gaaaactcag taacgttgtt ttgtcgtggg caggaaaatc agcatcaacc
     4021 cgctcataat acaggctact cccagtagtg ctttcagcga aaaagtccct tcccagccgg
     4081 gaagtaccat tgaagcaatc cataccagca cataactcat actgagcaag gcataggctt
     4141 tgcttagcgc aagtttatgt agcgttttat accaacaaaa aacggaaagc agataaccca
     4201 gcagcccgag taacagtatc cgggcatcca ggccaaaagc taacagagcc gcgataaaat
     4261 cccataagtg cgtcatcggc ggcagatgac ttgccgcaaa acccaggctt aattgcgcaa
     4321 ctgaggcaat aatgacgctg aataatcccc atatcaggcc cattacaccg tactcccgag
     4381 gatcacaatg ccaccaataa tgaacgccac cccacaccag tgacgcggcg ataccggttc
     4441 gtgccacagt tttactgcag ccagcgtcac ccagacaaaa ttcagactta acatcgggta
     4501 ggcaatgcct accggtacgt tctgcaagac cagcagccag agcaccatgg caagaccaag
     4561 acaagccagc gccagtccca gccacagcac gatatgtttg cgccgtttgt ttatcgccac
     4621 aaagcaggtt gcctgtttct gacacaactg cccggcaacg ctaagcaagc tggcaaagac
     4681 taatgttagc cagatcattt gggacgatac tgaatcagca ccagacgctc ctgacgatcg
     4741 atggcatctg cgggcggaat ggcgagactg ttgatatctt catcgcggtc aaccgaaagc
     4801 acgagagtaa taatcccctc ctgacgatgt tgattaagcc agtttgcgaa ctcatcaccg
     4861 ctgacaaatc tccctttcgc atccggataa ttaaggccgt atttcaactc acctgtctgg
     4921 cgatacatga tgatgtcatc gcgttgcagg ctccatgcca gacctgcggc aacaccgacg
     4981 ctatcagtaa gaatatagcg gcttggctga agtgattctt gtgtcatctc gacaaaaaat
     5041 tgcggatgtt ttccttccat aactctgtca ggaattgaaa atcctaccag caacgccagc
     5101 cccagcgggc aaagcgcggc aaaaggccag gtcttttcga cgtttgttaa ggtgtaccag
     5161 ccgaaaaatg cccatagcga aaaaatcgac caggcacaaa agactttata gctctcgaag
     5221 gtttgccaca ccggcgtgtt cattggtccc cacggggaga ccacaaatgt ggcaataatg
     5281 ccagtgacgc caaaagcgat gttaatccag ccattaatcc gcagcgccag aggattattt
     5341 tttgctgcca gcaaagcgta atgcgccatc agcattgcca gagatgcaaa gcaggaaaga
     5401 atataggtgg gcaatttacc tttagcgacg gagaaaaaca gcagcggcat tatcgtccag
     5461 ctcaacaaat agacggttgc ggaatgcttg cggtttttcc agcctgtgta cagtgcaccg
     5521 gggagtaatc ccagccacgg caggctaccg gcaatgatga ccggcacgta gtaccagaac
     5581 ggagctctat gttgggcatc atccagtgca aagcgttgaa tatgctcaac ccagaaaaaa
     5641 tagtgccaga agtcaggctc ccgctgcgct atcgccagtc cccaagggag aaccgtcagt
     5701 acgcaactga taaccgccag ccagccgtaa ataaagagat ctttccagcg tttttgcgtc
     5761 gctacccatg gcagcacgct taataccggc acggcaaggg cgagaaaacc tttggtcatc
     5821 acccccatgc cgcaggtgat tcccagcagt aaaaatcctg cgcttttgcc tttccacgtc
     5881 tgtgcctgca ttgccagcca gaagctgcac attcccgcca ccagccagaa tgcaataaac
     5941 ggatcgagca cggcataagt gccgatggca tagacaataa acaatgagag ataaattact
     6001 gtggcgagta gagccagacg tttatcgcgc cataagcgca gagtaaacca ggtcaccagc
     6061 gcggcagtta acagggtcgc aaagataacg cctgcccgca caccaaagtt attcgcgcca
     6121 aatagccatt gcccaatgct gttaatccag tatccggcaa tgggtttttc gaaataacgt
     6181 agccctaaca gatggggcac aatccagtcg ccggatgcca gcatttctcg actgatttcc
     6241 gcataacgcg tttcatcggg ttgccagagc agacgcgtgc tgatcggtaa caggtaatag
     6301 caggcaataa acgcgaagag gccgataagg taacgtaccg atttcatcag cgactacccg
     6361 caatttgttg gcaacccagc cagccttcac gtccggcaat atttccgcga acaacttgtc
     6421 cgagcggcaa cgtctctgat aacagttcgc ttaaagggca aaatgtcact ccttcctgag
     6481 ctgcgcgttt gaggagatcc acaaaattat gctgataagc gcagccttca acttctgcat
     6541 gaatggtata aaccggcgtg cctttatctc gcaggatgcg gttgagtaac caaccgttaa
     6601 aatcttctgc tttcacatcc cgaccaatca cttcatccca ggtgggtaag gtcaccggaa
     6661 tttgcgcagt gccagggttt cctgattcga gcaatggacg gaacggcatg gccccacgac
     6721 aatcgctgtt gtagcgcaaa tggaacgctt cttttgcttc gatcaccttc tggtcggcac
     6781 gccagcccgc agcggcagaa caggttaccg gttgaccgat aatctcttcc agagtgcgaa
     6841 gaccgcgtgc aatatcgtcg atcattgttt gccgatccca gttaccgcta cgggcttgcc
     6901 aggcatggtg atcccaggcg tgcaggccga cttcgtgatg tttagccgct tcacgaatga
     6961 tatcggcatt ggcatgacca atctctttac ctggccaggc cgtacctgcc agtaaaatat
     7021 cccagccata aagcgatgcc gcatttgagc gcagcatctt ccacaaaaac tgtggcttca
     7081 ccagtcgcca gagatggcgg cccatattgt ccgggccgac gctgaaaaaa atgctggcct
     7141 gaatattatg cttactcaag atttccagca gacgcggcac gccttcacgg gtgccacgaa
     7201 aggtatcgac atcaatgcgt aagcctactt tggtcatgat ggtttatccg taagatcaac
     7261 ggtgcgcagg aagaaatcca gcgtttcgtc gatggtttcc tgcatatcaa ttttcggctc
     7321 ccagtccagg cagcggtggg cattgcggat gctcggttta cgatgctcta cgtcctgata
     7381 tcctttgccg tagtagctgc tactttcgac aacgcgaaag cccgcaaacg gtgggaaatg
     7441 atggcgcagc ggatgttttt cgaagctcgc cagcagcatc tcgcccagtt cctcaatgct
     7501 cgcttcgttc tcaggattgc caatgttgat aatttcaccg tcgcagcgat ttcccgcatt
     7561 ttcgataatg cggtataacg cctcgatacc atcgcgaata tcagtaaagc agcgtttttg
     7621 ttttccgcca tcaatcagct taatcggtga accttctacc agattgagaa tgagttgcgt
     7681 aatagcgcgg gagctgccga ttcgtgccgc attaaggtta tccagtcgcg gtcccatcca
     7741 gttaaacggg cggaagaggg tgaactgtaa accctctttt tcgccatagg cccagatcac
     7801 ccgatcaagt aattgttttg ataccgaata aatccagcgt ggtttattca ccgggccgac
     7861 gattaaatta gaatggtcct catcgaagta tttatcgcta cacatcccat aaacttctga
     7921 agttgacggg aagatgattc gcttacggta cttcacgcag tagcggataa tgcgcagatt
     7981 ctcttcaaaa tcgagttcaa atacgcgcag cgggttgcgg gtatattcaa tcggcgtggc
     8041 tatcgccacc agcggcaaga cgacatcaca ttttttgacg tgatactcaa tccattcgga
     8101 atgaatactg atatcgcctt caacaaagtg aaaatgcgga tgattcagaa aacggcttat
     8161 cgcatcactg ccaatatcca gaccgtaaac ttcataatga tcttcgcgca gcaggcgttc
     8221 tgtcagatgg ttgccaataa agccattcac cccgaggatg agtacccggg tacggcgtcg
     8281 ggcggtgcag gcaggctggc tattcaagcg tgaaccttgc accaggccca gcgtctgcgc
     8341 taattgcgag ccctgcatag taatgccgtc gcccgcctgt ccggtgacga tttccagcgc
     8401 gccatcgcca caggcaatca gcagtggcgc aacagaaatc acgctccccg gctgtgcttt
     8461 gctggcatga ggatgaacac gcgacgacca gacggtgaat ttctgattgc caacatagct
     8521 gaaggcaccc ggccacggat cggcaacggc acgtaccatg ttgtgcagta cggatgccgg
     8581 tttatgccat tcaaggaaac tgtcatccgg cgttctgcga ccaaaacagg tggcttcgtt
     8641 ttcgcgctgg gcgatttcca gaatattacc gtgtttaatg gcgggtaatg tctgttccag
     8701 tagctggcgc gcggcatggc acaatttatg atgcagcgta atagcgatat cgtctggcgc
     8761 aatggcaatg cgcagttgcg ccacaatggc cccggcatcg gcacgtttca ccattcggtg
     8821 caatgtaacg ccagtttccg tttcaccgtt gaccagcacc cagttcagcg gcgcgcgacc
     8881 acgatatttt ggtaacagcg aaccatgcag attaaatgca cctgcgggag cgagctgcaa
     8941 aatttcgtcg caaataagat ggcgataata aaaagagaaa atcacatctg gcgacagttg
     9001 ggcaatgcgt tccacccaca gcggatgatt aacgtcatcc ggcgcataaa ccggaatgcc
     9061 tctttccgct gccagacgag ccaccgaacc ataaaaggct ttttcaccgg gattatcggt
     9121 atgggtaaaa atggcgctaa tttcgtaacc ggcagccagc agagcttcaa taccgaggca
     9181 tcccatatcg tggtaggcaa aaacgacggt tttcattatt cattttcctt gctggatgga
     9241 cggataactt gctgaacaaa atagcggggg cgggcgcgga catcggtgta gatcctgccg
     9301 atatattcac cgagtaatcc catgccgata aactgagcgc caataaaagt aaacagcacg
     9361 gcaaatagca taaagacgcc ttctgccgcc cattgtggtc cgaaggtcag gcgtaaaatc
     9421 accagcagca ccgcaatgct aaaacctcca atcgcaataa tgctgccgag cagactcagc
     9481 atacgtagcg gcgttgtggt aaggcaggtc accaggtcgt acatcaaatt aatcaggcgc
     9541 ataaaactgt atttggattc accaaactca cgctcggaat gatgtactgg aatttcaatg
     9601 gcacggcggg cgaagatatt cgccagaatc gggataaagg tgctacgttc atggcagtgc
     9661 aacatcgcat cgacaatatg acggcgatag gcgcgcagca tacaaccgta gtcacccatc
     9721 gctttgccgg tggtgcgctg aataagccgg ttaatcatct tcgaagctgt tttacgaaac
     9781 cagctgtcct ggcggttctg gcgtacagtc cctaccacgt cgtaaccttc atcggctttt
     9841 gccaccaggc gggggatttc ttctggcgga ttctggagat cggcatcaag ggtaataatt
     9901 aagtcgccgg taacgtgact gaatcccgcc ataatcgctg aatgttgccc gtaattgcgg
     9961 ttaagcaaaa tagacacaat atggctgttc tccgcttgtg aggcttcgac cagcatatgc
    10021 gcggaattat cgctactgcc gtcatcaatc agcaggatct catactcttt ccccaacgat
    10081 tcacaggctg tggtggtgcg cctgattaat tccggtaagc tttcctgctc gttataaacg
    10141 ggaataacca ccgagacttt cttaacaggg tggatttcaa acatggctta ttgtcctgcg
    10201 agttgctgaa gggctgtgat gacacggtcg gcatcggcgg tagtcatatc cgggaacagc
    10261 ggcaaagaac agatgcgttc gctattccat tcggtattcg gtaacgacag cgtgggaaaa
    10321 cgctcgcgat aatatttttg tgtgtgagcg gcgcggaaat gtaacccggt accaatgcct
    10381 ctttctttta acgcttccat caacgcatcg cgactgatac cacaacgttg ttcatcgaca
    10441 cgaataataa acagatgcca ggcgtgaacg tgcggccagg cgggaaggct taatggctga
    10501 aaggggagag ctgccagtgc ttgctgatat tgctgggcaa tttcgcgccg acgggtgttg
    10561 aggtgctcta attttgctaa ctgtgtcagg gcaattgcgg cgttaatatc ggtcagattg
    10621 tacttatagc ccggtgttaa gacttcagcc tgcggtgcac ggccccaggt ttgtctgtca
    10681 taggcatcga cacccagacc gtgaaatttc agcatccgta gctggcgggc aaggttttca
    10741 ttatcagtta caatcaggcc accttccgca caggtaatat ttttaatggc atgaaatgaa
    10801 aaaatagtgg taccttttgc gccaatatgt cgccctttgt aatacgtacc gacggcatgg
    10861 gcagcatctt cgataactgc gatgccgtaa cgttcgccaa tggcgcgaat ggcgtcaata
    10921 tctgctggcg caccggcata atgcaccgga atgatggctt tagtgcgtgg cgttatggct
    10981 gactcgatcg cttcaggcgt gaccatcggc gtatcgcggt cgacatccac cattaccggc
    11041 gttgcaccca acaaggaaat catattgagg gttgaaaccc aggtcaggga aggcgtaatc
    11101 acttcatcgc ccttgccaat ttccaacgcc attagcgtga tatgcattcc ggcggtggct
    11161 gaactgaccg cgatggcatg ctgatttccc gtcaactggc aaaaggcttg ctcaagcgcc
    11221 tgattcttcg gaccggttgt gatccaaccg gattcgagaa cctctttaac tgcagcgagt
    11281 tcctccacgc ccattgctgg tcgcgaaaaa ggcaaaaatt ctgacattgc ttttccttcc
    11341 gccattgaat acctgtccac ttatatttgc tataaagagt gttgtgtata ttttgccatt
    11401 tggagc
//
LOCUS       DAZGJW010000141        11096 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZGJW010000141 DAZGJW010000000
VERSION     DAZGJW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.141"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            54..593
                     /locus_tag="ACHKIK_003980"
                     /pseudo
     CDS             54..593
                     /locus_tag="ACHKIK_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
     gene            627..1706
                     /locus_tag="ACHKIK_003981"
     CDS             627..1706
                     /locus_tag="ACHKIK_003981"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO9085057.1"
                     /translation="MEIVPGEQPGESDILITRKQDKYWRLGFSLDDSGTRSTGRYLGG
                     ITFSLDNPFSLSDLLYVSAIHNFPHYGGKGSKNYTAHYSVPFGYWQFSVTASDYDYHQ
                     TVAGAVEDYQYSGESQNLGMQLSRVLHRNGTQKTVLTYDVQARRSRNYINDTEIQVQR
                     RQTAAWRLGLQHRHYISQAILDAGVSWQRGTRWFGAQPAPEETFGEATALSKILQLNA
                     SFNLPFTLAGQPFSYSFRYQRQLSNTPLTPQEQFAIGNRWTVRGFDGERTLNASNGWY
                     IRNDLAWRTPLPEQALYMGMDYGEVGGAGSEYLVGRHLAGGVVGLRGQLFSTDYDVFI
                     GRPFSKPAGFMTSDVTAGFNLFRDF"
     gene            1716..2243
                     /locus_tag="ACHKIK_003982"
     CDS             1716..2243
                     /locus_tag="ACHKIK_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFO9085058.1"
                     /translation="MRNGKYDVLSPLYSGEPVNEAEVLGAAVWLWMHSPLHRDAPLHT
                     LPDLLLPVIKHRQYVVATEQGRPVFFMSQAWLSPEAEARYLTQPAILMPQSDWNSGDR
                     MWVCDWVAPFGHTPDMSRLVRQDIFPRLCWRSLYHRGQQTGLRVMNFRGAQVSREEAR
                     QWRVQHPLAVDVPER"
     gene            2259..3491
                     /locus_tag="ACHKIK_003983"
                     /pseudo
     CDS             2259..3491
                     /locus_tag="ACHKIK_003983"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
     gene            3549..3953
                     /locus_tag="ACHKIK_003984"
                     /pseudo
     CDS             3549..3953
                     /locus_tag="ACHKIK_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4098..11069
                     /locus_tag="ACHKIK_003985"
     CDS             4098..11069
                     /locus_tag="ACHKIK_003985"
                     /inference="COORDINATES: protein motif:HMM:NF024728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 25 domain-containing
                     protein"
                     /protein_id="HFO9085059.1"
                     /translation="MESAGRLDISAGSLNNHQGTVVSDGLSVTLDGALDNTSGRLLSQ
                     KTLSVSGSELVSDDGLIQSGSDMTLDVQDGVLSNRNTKTRGGISSAGTLTVRAGMLNN
                     QQGFMVGQKDMTLNAGTLDNRQGVLGSQASLQISSGTLMNQKGALKAGTDMLLSGGDV
                     SNQEGTLAAGGDLNTHLNVLENQQGTVVSNGNSRLDVTRFDNQGGRLVAQQSLTLSST
                     DIINDASGLIQSGASLNLRADTLSNRNSGDRGGVISQGAMTLNAGTLDSTAGVLLSGD
                     ALSLTAGVVNNTSGQVVANGLLGWNSQALNNQSGLIQGKGISINTAGQTLDNRGGTLN
                     SLQELTVSTGAMDNRGGTVGAKTTADLSTTSLDNREGGRLVSEGELRLHTGGLQNSHG
                     QIQSVGDILFDSVRGVVDNVSGLIRSGSAITLNALQFINRHTQNTGQGLEAQTIHITT
                     QDLDNQEGSILADRALTVMADRTLSNNDGVLSSGATLSVSGRQLAFSNRDGVVKAGQS
                     VSVDAGQLGGDGKLLSPGDITLKSNTTFSNSGQTIANGNLTLSVNGDVSNTGSLLAGS
                     RLDLNSIRLENTEKGEISAGQTWLNVTDTLLNRGLIDGKYTRLQVNTLTNSGTGRIYG
                     DAVGVSAATLNNLDENGVAATLAGRERVDLGVQTLNNRTHSLIYSAGDMHIGGTLDAN
                     GAATGKAGVLNNHSATIEAAGYLALSAGQINNVNDHFTTERVVVSTEKVTEYQLSGSD
                     KRWSAGEPGVYVDNDSSNSLKKLHTPEGARDKFTQYDYTRTVEETRVKDSDPGKILSG
                     AGMTIVADKLLNDKSQVVAGGLLTIPSGSVENVSVSGERHVTDSGTSTYYYRIRKKGK
                     DKQGEKTSQYTPPTVIQSITLKPGELTSHGQIQGSQVTLSPLKPQGTDVQTGQAGNVD
                     AAVAATDRIPLRPVVSAGEPVILLPGQQFEVSTPQGSIHVAGPDTRLPDSSLFKTNPA
                     VNVPYLVETDPRFTNQKTWLGSDYMQKAFSQNGDNMLKRLGDGFYEQRLIREQVVALT
                     GQRYLDGYSNDEEQFKALMDAGIAFGKQYNLTPGVALTAEQMALLTGDIVWLVNTTVT
                     LPDGSTQTVQVPQVYARVKPGDVNSAGALIAGRDMVMKLDGDLFNSGKLAGKQTVQLS
                     AENIHNQAGTIQGANVSLTAWTDINSTGGLLQATDSLLAMAGRDISLTTTTRTAQSDA
                     GQNHFERTSIDSVAGVYVQNDQGRLVLQAGRDMNLTAATVVNQGKDSLTQLSAGGDMT
                     LSTVTTSAQDNITWDKNNRLSQGVTQSTGSTLAGNGDVTLTAGRDMTSQAASLSAQKG
                     LALMAGHDVTLTGAQNTSSLDEYHKVTGSSGMLSKTTTTTHDVTDRRTMTGSELNGDT
                     VSIGAGHNLNVTGSSVAGDNRVSLAAGNNLNIGTLAESNRETHLKQEKKSGLMSSGGV
                     GFSVGSQSLKVTDTATDTTQKGSTVGSVHGDVSLQAGNRLTVNGSDLIAGRDMALSGK
                     EVSITAATDQHVQTHTVEQKTSGLTLALSGTAGSALNTTVETVQAAKSAGNSRLEALQ
                     GVKAALSGAQAVQAGRLADAQGADAENNNTVGISLSYGSQSSKSEQQSEQTVAKGSTL
                     TAGNHLSIQATGSGVKGVDGDLTIQGSQIKAGNNVLLQANRDVNLVSAENTSKLEGKN
                     TSSGGSVGVGVGVGSGGWGISVSASANQGKGSEKGNGTTHTETMVDAGNRLTIISGRD
                     TTLTGAQAGGETVKVDAGRHLTLTSEQDSDRYDSKQQNASAGGSFTFGSMSGSASVNL
                     SRDKMHSNYDSVQEQTGIFAGRGGFDVTTGQHTQLNGAVIASTATADKNRLDTGTLGF
                     SDIENRADFKTEHQSAGLSTGGSVAGNFLGNMANNLLVGANHEGHADSTTQSAVSAGN
                     ITIRDTQSQKQDVADLNRDAAHANQTLSPIFDREKEQQRLQQAQLIGEIGNQVADIAR
                     TEGQIAGEKAKRDPAALNQARAELEAAGKPFTEQDVAQRAYNNGMAASGFGTGGKYQQ
                     AIQAATAAVQGLAGGNLSAALAGGAAPYIAETIKQTTPDGAGRVAAHAVVNAALSLAQ
                     GKNALAGAAGAATGEVVGMIATQMYGKPVSELSETEKQTVSTLATVAAGLAGGLVGDS
                     GASAVAGAQSGKTTVENNALSELVPPRVQQDTSLAIDLGQKGQGHEEINNAIDASHIG
                     PSWGTEYKVKTNGKIEVSGGIGPALKGEMKVNNDYFSVSTGYTKEFGAKAGASIGIDF
                     GPYDFGVFGDLNRDYSSSIGYGPGSVGLSVGQDGFGFSFSIGPSIGFTGSSTVPHSEK
                     IEMSGDTTKEIYHYDFK"
BASE COUNT         2668 a         2705 c         3461 g         2262 t
ORIGIN      
        1 ctgaggaaca aagcgtattc cggcgctgtc cgtccttgct gtcactgaca ctgatgtctg
       61 ttttattatc gcctttatct cttcaggccg cggatatccg gcgtagtgga gaggatacat
      121 ttatcattca gcagcagcgt caggaagccc ttgagcaaca actgacgcct tcagcccctg
      181 atgttcgcct ttctgcacct ggctcttttg cccgtaagat taattttcct gttgaaacgc
      241 cctgttttca gattaaacag acggaactgg agggggctga tgcgttacca cactggttgc
      301 ctttacaaaa aatcgccaac ggggcggtcg ggcattgcct gggggcgaaa ggaattaatc
      361 tgctgatgag tacattgcag aaccgtctgg tcgatcatgg ttatgtcacc acccgggttc
      421 tggcacctgc gcaggattta aaaagcggta tcctccggct ggttattatt cccggtgttg
      481 tgcgatatgt gcgtctgaca ccggacagtg gtgactatat tcagttgtat tcctcattcc
      541 cggcacacga aggctctctg ctggatttac gggacattga gcaggggctg gagaatatgc
      601 aacgactgcc gggtgtgcag gctgatatgg aaattgtgcc cggtgagcag ccaggggaaa
      661 gtgacattct tattacccgc aaacaggaca aatactggcg actggggttt tcgcttgatg
      721 attcaggcac ccgcagcaca gggcgttatc tcggtggtat aacattttct ctggataatc
      781 ctttttccct gagtgacctg ctgtatgttt ctgccatcca taattttcca cattacgggg
      841 gtaagggcag taaaaactac acggcccatt actctgtgcc tttcggctac tggcagtttt
      901 ccgtcaccgc cagtgattac gattaccatc agacggtggc tggtgcggtt gaggattacc
      961 agtacagtgg cgagagtcag aatctgggta tgcagctgag ccgggtgctg catcgtaacg
     1021 ggacgcagaa aacggtgctg acctacgatg ttcaggcccg caggtcgcgt aactacatta
     1081 atgatacgga aatccaggtc cagcgccggc agacggcggc ctggcgactt ggactgcagc
     1141 accgccatta catttcgcag gccatactgg atgccggcgt cagctggcaa cggggaaccc
     1201 gctggtttgg cgcacagccg gcgccggaag aaacgttcgg tgaggcaacg gcgctcagta
     1261 agatcctgca gttgaatgca tcctttaatc tgccctttac ccttgcaggg cagcccttca
     1321 gttattcctt ccggtatcag cgccagctga gcaatacccc gctgactcca caggaacagt
     1381 ttgccatcgg taaccgctgg acggttcgtg gttttgacgg ggagcgaacg ctgaatgcca
     1441 gtaacggctg gtatatccgc aacgacctgg cctggcgtac gccactgccg gagcaggcgt
     1501 tatatatggg aatggattac ggcgaggtgg ggggagccgg cagtgaatat ctggtgggac
     1561 gtcatctggc cggtggtgtt gtggggctga gggggcaact gttcagtacg gattatgacg
     1621 ttttcatcgg tcgtccgttt tcaaagccgg cgggttttat gacgagtgat gtgacagccg
     1681 gttttaatct gttccgggat ttctgagaaa gtttcatgcg taacgggaaa tatgacgttc
     1741 tgagtcccct gtattccggt gagccggtca atgaggcgga agtcctgggg gcggccgtct
     1801 ggctgtggat gcactccccc ctgcatcgtg atgcgccact gcataccctg ccggacctgc
     1861 tgctgccggt gattaagcac cgacagtatg tggtggcaac agagcagggg cgaccggttt
     1921 tcttcatgag ccaggcctgg ctcagcccgg aggcagaggc gcgttatctt acgcagccgg
     1981 caattctcat gcctcagtcg gactggaaca gcggcgaccg gatgtgggtc tgtgactggg
     2041 ttgccccttt tggacacact ccggatatga gtcgtcttgt ccggcaggat atttttcccc
     2101 gtctgtgctg gcgttcgctg taccaccgtg ggcagcagac cgggttgcgg gtgatgaatt
     2161 tccggggtgc acaggtcagt cgtgaagagg cgcgccagtg gcgtgtgcag cacccgcttg
     2221 ctgtggatgt gccggagaga taagtcaggt ttatcagaat gaacagaaat tgttaccgca
     2281 ttatttttaa caaagcccgg ggaatgctga tggtggtggc agatattgcc cgttccggtc
     2341 gcgccggtac atcactgtcc tcccgcacag gatatccaca caggcaacgt atctgccgtg
     2401 tcacgccgct tgcattctct ttgtggctgg cgtcggggat ggtgcattct gtcagtgctg
     2461 caggcattgt cgcagaccat gacgcaccgg ggcatcagca gcccacgatt atgcagacgg
     2521 ccagcggcat tccgcaggtg aatattcaga cgccttctgc cggtggggtt tcacataaca
     2581 catacagcca gttcgacgtc ggtaacaagg gtgtcattct gaataatgcc cataacaatg
     2641 tgcagactca gctgggtggc atggtggccg ggaacccgtg gctggcgaag ggagaggccc
     2701 gaatcattct taatgaagtg aacagtcgca atcccagcca gttgaacggt tttgttgagg
     2761 ttgccgggaa aaaggcgcag gtggtgattg cgaacccggc cggcatcagt tgcgacgggt
     2821 gcggttttat taatgcgaac cgggccacgc tgaccacggg gcagccgcag atgaaaaacg
     2881 gcagtctgac cggattcagt gttgaacgtg gtgagattca gattacgggc aaagggatgg
     2941 atgcctcgcg cacggattat accgatatca ttgcccggtc tgtgaagatt aatgcgggta
     3001 tctgggcgca ggacctgaaa gtgaccacag gacgtaacaa tgtggatatc gcgcacgggc
     3061 agattgagaa aaagaccgga gatgtctctt cacagcctca gatggcactg gatgtggcca
     3121 gtcttggcgg aatgtatgcc ggaaaaattc gccttgtcgg tacggaaacg ggggtgggtg
     3181 ttcgtaacgc cggtcatatt ggtgcacagg caggggcggt taccctgacg gctgacggac
     3241 gaattgaaaa cagtggcagt atcagtgcga aaacggatgt gcatctggcg acaacccggg
     3301 agctgcataa cagtggttct gtttatgcgg ggcaggatac gcagatacag agtgatggtg
     3361 tattcaccca tacaggctct gtggcatcac gtcgcaacac ccggattcag acagcgcgtc
     3421 tgatcggcgg tgagcggagt ctgctggccg ccggggtgaa agatgacgga cgtctggcgg
     3481 cagcgggcaa cctgactgtc agtaccaccg gcgaactggc ggcacacgga caggttctgt
     3541 ccggtgggga catgcaactg aaggggcagg ggctggattt aggcaacagc cggatacagg
     3601 gacaacatac tgagctgaat gcaaccagtg gaaatctgtc tacacagaat gcgcaactga
     3661 gtgccggtac gctttccgcc cggactgccg ggcagttcag caataatggc ggtacgataa
     3721 atgccgacac actgcagata tcggcacaaa gcctgtcaaa tcgtaaaggc agtctgattc
     3781 agacgggaac aggggatttt tcgctgagtc tgccgggaag cgtggataac cgggaagggc
     3841 tgcttgcggc aaatggcgcg gtgcgtctgg atgcactgag ccttgataat cgcaagggga
     3901 aagtgcaggc ggcgcagtca ggcagtctgc aggtgaagac aaccggtgcc gtggataacc
     3961 agcagggacg tctgcttgcc agcagtgaca tcctgattaa tacacaggcg ctgaataacg
     4021 acaacggact gatttcagct gctgccggaa cgggcaggat aaaaacacag cagtctgtca
     4081 gcaatacgga gggacgaatg gagtccgccg gcaggctgga tatttcggca gggagtctga
     4141 ataaccacca gggtacggtg gtgtctgacg ggctgtccgt gacgctggat ggtgcgctgg
     4201 ataatacgtc cggcagactg ctttcacaga aaacgctgtc tgtgtcaggc agcgaactgg
     4261 tctctgatga tggtctgatt cagtccggca gtgacatgac gctggatgta caggacgggg
     4321 tacttagtaa ccggaacacg aaaacacggg gtggtatcag cagcgccggt accctgacag
     4381 tgcgggctgg tatgctgaat aaccagcagg gatttatggt gggtcagaag gatatgaccc
     4441 tgaacgccgg aacgcttgat aaccgtcagg gcgtgctggg gagtcaggcg tcattacaga
     4501 tatcgtcagg tacgctgatg aatcagaaag gtgcactgaa ggccgggacg gatatgttgc
     4561 tgagtggtgg cgatgtcagt aatcaggaag ggacgcttgc cgccggcggg gacctgaata
     4621 ctcacctgaa tgtgctggaa aatcagcagg ggacggtggt cagcaacggt aacagcaggc
     4681 tggatgtcac acggtttgat aatcagggcg gcagactggt ggcacagcag tcactgaccc
     4741 tttcctcgac ggatatcatc aacgatgcca gcggcctgat acagagcggt gcatcgctga
     4801 atctccgtgc tgacacactg agtaaccgta acagtggtga ccggggcggg gttatcagtc
     4861 agggggcgat gacgctgaat gccggcacac tggacagcac tgccggtgtg cttttgtcgg
     4921 gagatgctct gtccctgact gcaggcgtgg ttaacaatac atccggtcag gtggtggcaa
     4981 acggcctgct cggctggaac agccaggcgc tgaacaatca gtccggtctt attcagggga
     5041 aaggcatcag cattaatacc gcaggccaga cgctggataa cagaggcggt acgctgaaca
     5101 gcctgcagga actgaccgtc agtaccggag caatggataa ccggggcggg accgtggggg
     5161 cgaaaacgac agctgacctg agcaccacct cgctggataa ccgtgagggt ggccggctgg
     5221 tcagtgaagg tgaactccgt ctgcatacgg gaggcctgca gaacagccac ggacagatac
     5281 agtctgtggg cgacattctg ttcgacagtg tacggggcgt tgtggacaac gtctctggtc
     5341 tgattcgcag tggttctgcc attacgctga acgccctgca gtttatcaat cgccacacgc
     5401 agaataccgg ccaggggctg gaagcgcaga caatacacat cacgacgcag gaccttgata
     5461 accaggaggg cagtattctg gcagacaggg cgcttacggt tatggcagac aggacgctca
     5521 gtaataatga cggcgtgctc tcttccggcg cgacattgtc tgtcagtggt cgtcagctgg
     5581 cgttttcgaa ccgtgacggt gtggttaagg ccgggcagtc agtctctgtg gatgctggtc
     5641 aactcggtgg tgacgggaaa ctgctttccc cgggcgatat aacgctgaaa agtaacacaa
     5701 cgttcagcaa cagtggtcag actattgcga acggaaacct tacgctttcc gttaatggcg
     5761 atgtctctaa taccggcagt ctgctggcag gcagccggct tgacctgaac agcatccgcc
     5821 ttgaaaacac agagaaaggt gaaatcagtg ccgggcagac ctggcttaac gtgacggaca
     5881 cactgctgaa ccgtgggctg attgacggga aatatacgcg tcttcaggtg aacaccctga
     5941 cgaattccgg taccgggcga atctacggcg atgcggttgg tgtcagcgct gccacattga
     6001 ataatctgga tgaaaatggc gtggcggcga cgctggccgg gcgtgagcgt gtggacttag
     6061 gggtacagac cctgaataac cggacgcaca gcctgattta cagtgcaggc gatatgcata
     6121 tcggtggcac gctggatgcg aatggcgccg ccacggggaa agccggtgta ctgaataatc
     6181 acagtgccac aattgaagcc gccggatatc tggcactgag tgccggacaa atcaataacg
     6241 tcaatgacca tttcaccacg gagcgcgtgg tggtttccac ggaaaaggtg actgagtatc
     6301 agctcagcgg ctcagataaa cgctggagcg ccggtgaacc gggcgtttat gtggataacg
     6361 attccagtaa ctcactgaaa aaactgcata caccggaagg tgccagagat aaattcacgc
     6421 agtacgatta cacccgtacc gtggaagaaa cccgggtaaa agactccgac ccgggaaaaa
     6481 ttctgtccgg tgccggcatg acgattgtgg cagacaaact gcttaatgat aagagtcagg
     6541 ttgttgcagg cggcctgctg accattccgt caggaagcgt ggaaaatgtc agtgtcagtg
     6601 gcgagcggca tgttacggac tcaggaacat ccacgtatta ttaccgtatc aggaaaaaag
     6661 gaaaggataa gcagggggag aaaacctctc agtacactcc gcccactgta attcagagca
     6721 ttacgctgaa gccgggagag ctcaccagcc acggacagat tcagggcagc caggtcacgc
     6781 tttctcccct gaagccacag ggaacggatg tacagacagg acaggccgga aatgtggatg
     6841 cggcagtagc cgctactgac cggataccgc ttcgcccggt ggtgtctgcc ggtgaaccgg
     6901 tcattctgtt gccggggcag cagttcgaag tcagtacacc tcagggcagt atccatgttg
     6961 ccgggccgga tacccgtctg ccggacagca gtctgtttaa aacaaatccg gcagtgaatg
     7021 tgccatatct ggttgaaacg gacccgcgtt ttacgaacca gaagacgtgg ctgggcagtg
     7081 attacatgca gaaggcgttt tctcagaacg gggataacat gctcaaacgc ctgggggatg
     7141 gtttttacga acagcgtctg attcgtgagc aggttgtcgc tctgaccgga caacggtacc
     7201 tggacggata cagtaatgat gaagagcagt ttaaggcgtt aatggatgcg ggtattgcat
     7261 ttggtaagca atacaacctg acaccaggtg tggcgctgac tgctgagcag atggcgttac
     7321 tgaccggtga tattgtctgg ctggttaaca ccacggtgac actgccggac ggcagcacgc
     7381 agacggttca ggtacctcag gtgtatgccc gtgtaaaacc cggtgatgtg aacagtgccg
     7441 gtgcgctgat tgccggcagg gacatggtga tgaagctgga tggtgacctg tttaacagcg
     7501 gtaaactggc agggaaacag accgtgcagc tcagtgcaga gaacattcac aaccaggccg
     7561 gcaccattca gggagcaaat gtcagcctga cggcatggac ggatattaac agtaccggcg
     7621 ggctgctgca ggccacggac agcctgctgg cgatggccgg acgggatatc agtctgacca
     7681 ccaccacccg tacggcacag agtgacgccg gacagaacca ttttgaacgt accagtatcg
     7741 acagtgtggc cggcgtgtat gtgcagaacg accagggcag actggtgctg caggccggac
     7801 gggatatgaa cctgacggct gcaacggtgg tgaatcaggg taaggacagc ctgacgcaac
     7861 tcagtgcggg cggggatatg acgctttcca cagtgaccac atccgcacag gacaatatta
     7921 cctgggacaa aaataatcgt ctgtcgcagg gcgtcacaca gagtacgggc agcacgcttg
     7981 ccggtaacgg tgatgtcacg ctgaccgccg gacgggatat gacgtcacag gcggcatcgc
     8041 tgtcagctca gaaaggcctg gccctgatgg cgggtcatga cgtgacgctg accggtgcac
     8101 agaataccag ctcgctggat gaataccata aagtgaccgg cagcagcggg atgttatcga
     8161 aaaccaccac aaccacgcat gatgtcactg accgccggac catgacgggc agtgaactga
     8221 atggggatac ggtcagtatc ggcgccggac ataatctgaa cgtcacgggc agcagtgtgg
     8281 ccggggataa ccgtgtctcc ctggctgccg gaaataacct gaatatcggt acgctggcag
     8341 aaagcaaccg tgaaacacat ctgaaacagg agaaaaaatc cgggctgatg agctccggag
     8401 gtgtgggatt cagcgtgggc agccagagtc tgaaagtcac ggacaccgca acagacacca
     8461 cacagaaagg cagcacggtg gggagtgttc acggtgatgt ttcccttcag gcaggtaacc
     8521 ggttgaccgt caacggctct gacctgattg ccggcaggga catggcactc agcggtaagg
     8581 aggtcagcat taccgccgcc acagaccagc atgtacagac acatacggtg gagcagaaaa
     8641 cctccggtct gacgcttgcc ctgtccggca cagcgggcag cgccctgaat acgacggtgg
     8701 agaccgtaca ggcggcgaaa tctgccggaa acagccggct tgaagccctg cagggggtga
     8761 aggccgcgct ctcgggggct caggccgtgc aggccggacg actggcagac gcgcagggag
     8821 ccgatgccga aaataataac acggtgggca tcagcctctc ttacggcagc cagtcttcga
     8881 aatcagaaca gcagtcagag cagacggtgg cgaagggcag tacactgacc gcaggtaatc
     8941 acctcagtat tcaggccacc ggctccggcg tgaagggcgt ggatggtgac ctgacgatac
     9001 agggcagtca gataaaggcc gggaacaacg ttctgctgca ggcaaaccgt gacgtgaatc
     9061 tggtatcagc agaaaacacg tcaaagctgg aaggaaagaa cacatccagt ggtggcagcg
     9121 tgggtgtcgg tgttggtgtg ggctccggag gctggggtat cagtgtttca gccagcgcaa
     9181 accagggtaa gggcagtgaa aaaggcaacg gtaccaccca tacagaaacc atggtggatg
     9241 cgggaaaccg gctgaccatt atcagcgggc gggacaccac gcttaccggc gcgcaggctg
     9301 gcggtgaaac ggtgaaagtg gatgccggtc gtcatctgac actgaccagt gagcaggaca
     9361 gtgaccgtta tgacagtaag cagcagaatg ccagcgccgg aggcagcttc actttcgggt
     9421 ccatgagtgg ttcggcgagt gtgaacctca gccgggataa aatgcacagt aactacgaca
     9481 gtgtacagga gcagacgggg atttttgccg gcaggggcgg gtttgatgtc accaccggac
     9541 agcatacgca actgaacggt gcggtgattg cgtccacggc aacggcagat aaaaacaggc
     9601 tggataccgg cacactgggc ttcagtgaca ttgaaaaccg ggccgacttt aaaacggaac
     9661 atcagagtgc agggctcagt actggtggca gtgttgccgg aaatttcctg gggaacatgg
     9721 caaacaatct gctggtcggt gcaaatcatg aaggccatgc ggacagtacc acgcagtcgg
     9781 cagtgtctgc cgggaatatc accatccggg atacgcagag ccagaaacag gatgtggcag
     9841 acctgaaccg tgacgcagca catgcaaacc agacgctgtc ccccatcttt gacagggaaa
     9901 aagagcagca gcgactgcag caggcgcaac tcattggtga gattggtaac caggtggcag
     9961 atattgccag aacggaagga cagatagccg gtgagaaagc gaagcgggac ccggcagcac
    10021 tgaatcaggc ccgcgcagag cttgaggcag caggaaagcc gtttacggaa caggatgtgg
    10081 cacagcgggc ctacaataac ggtatggcgg cctcaggttt tggaacggga ggcaaatacc
    10141 agcaggcgat acaggccgca acggcagcgg tacagggact ggccggcggt aatctgagtg
    10201 cagccctggc aggtggtgca gcgccgtata ttgctgaaac tatcaaacag accaccccgg
    10261 atggtgcggg acgtgtggca gctcatgcag tagtgaatgc agccctgtct ctggcacagg
    10321 gtaagaatgc tcttgctggt gccgcaggcg ctgccaccgg cgaggtggtg ggaatgatag
    10381 ccacacagat gtacggtaag ccggtgagtg aactgagtga gacagagaaa cagacggtct
    10441 ccacgctggc aactgttgct gccggcctgg ccggtggtct ggtgggagac tcgggggctt
    10501 cagcggtagc cggcgcacag tcgggtaaga cgactgttga gaataatgcg ctgtctgagc
    10561 ttgtgccgcc tcgcgtacag caggatactt ctctggcaat agatctagga caaaaaggac
    10621 aaggccacga agaaattaac aatgcgatag atgcttctca tattggtcct tcatggggga
    10681 cagagtataa agtcaagact aatggaaaga ttgaggtttc gggtggaata ggtcctgccc
    10741 ttaaaggtga gatgaaagtt aacaatgatt atttctctgt ttctacagga tatacaaagg
    10801 agtttggtgc taaagctggg gcttcgattg gtattgattt tggcccatat gatttcggtg
    10861 tatttggtga cttaaatcgt gattattcaa gcagtattgg ttatggccct ggttcagtag
    10921 gtctgtccgt tgggcaagat gggtttggtt tttccttctc tattgggccg agtattggtt
    10981 ttacaggttc ttccactgtt cctcattctg aaaaaataga gatgagcggt gatacaacaa
    11041 aggaaatata ccactatgat ttcaagtgat ataatactta atgcatcatc attgat
//
LOCUS       DAZGJW010000142        11046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZGJW010000142 DAZGJW010000000
VERSION     DAZGJW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.142"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(131..850)
                     /gene="dcuR"
                     /locus_tag="ACHKIK_003986"
     CDS             complement(131..850)
                     /gene="dcuR"
                     /locus_tag="ACHKIK_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO9085060.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(847..2478)
                     /locus_tag="ACHKIK_003987"
     CDS             complement(847..2478)
                     /locus_tag="ACHKIK_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO9085061.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2659..2889
                     /gene="yjdI"
                     /locus_tag="ACHKIK_003988"
     CDS             2659..2889
                     /gene="yjdI"
                     /locus_tag="ACHKIK_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO9085062.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            2901..3173
                     /locus_tag="ACHKIK_003989"
     CDS             2901..3173
                     /locus_tag="ACHKIK_003989"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9085063.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3400..3696
                     /gene="ghoS"
                     /locus_tag="ACHKIK_003990"
     CDS             3400..3696
                     /gene="ghoS"
                     /locus_tag="ACHKIK_003990"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO9085064.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            3724..3897
                     /gene="ghoT"
                     /locus_tag="ACHKIK_003991"
     CDS             3724..3897
                     /gene="ghoT"
                     /locus_tag="ACHKIK_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO9085065.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(4016..5533)
                     /gene="lysS"
                     /locus_tag="ACHKIK_003992"
     CDS             complement(4016..5533)
                     /gene="lysS"
                     /locus_tag="ACHKIK_003992"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO9085066.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(5770..7227)
                     /gene="dtpC"
                     /locus_tag="ACHKIK_003993"
     CDS             complement(5770..7227)
                     /gene="dtpC"
                     /locus_tag="ACHKIK_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO9085067.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMAVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(7286..9433)
                     /gene="cadA"
                     /locus_tag="ACHKIK_003994"
     CDS             complement(7286..9433)
                     /gene="cadA"
                     /locus_tag="ACHKIK_003994"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO9085068.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(9513..10847)
                     /gene="cadB"
                     /locus_tag="ACHKIK_003995"
     CDS             complement(9513..10847)
                     /gene="cadB"
                     /locus_tag="ACHKIK_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO9085069.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
BASE COUNT         2991 a         2605 c         2623 g         2827 t
ORIGIN      
        1 aatttttagc gatgatggag ggataattat atttgatctg gcacaagttt tactgatgaa
       61 ggatgtaact tgtgccaggg gtattttgca ttacggtaat aattattact tacagataac
      121 cagcgatccg ttattggcaa tattgtttca gtagtgagta gtgttctgcc tgaatacggt
      181 aacggtaaac cggacgcccc gtgacgccat aatggatact ggtgaacaag atgtggcagt
      241 tgaccagcca gatgaggtat ttacggcagg aaacacgcga gatgttaacc tcgttagcca
      301 gctcgtcggt tgaaaactca tagtcctgat gcgcgtcaat ccactggcac agcgtgcgta
      361 aagtctgcgg cgttaagcct tttggcaagc gacgaggatc ctgttcgttg gagctgctgc
      421 cgtggattag ctgatcaagc tcggcctggt cataatactg atgtttttcc agcgccattt
      481 tcttttgccg ccagccggtg agcgcctctt caaagcggga agcctggaag ggtttgatca
      541 ggtaatccac gacaccgtaa tgcagcgaat ctttaatggt tgccgcatcg gctgcggagg
      601 agatgacaat cacatcactt ttgcaacgcg cgttatgcag gacaggcagt aaatcgagcc
      661 cgttctcttt ttgcatatag atatcgagca atatcaggtc gataggcgta tcgctattga
      721 agataatctc tttggctttc tccagcgtcg aggctgttcc acagcattga aagcctggga
      781 tttgtgctac gtatcggcga ttcagctccg cgaccattgc gtcgtcatcg ataattaata
      841 cattgatcat ctgttcgacc tctccccgtc ccagggtatc tggacaaaaa attgtgtgaa
      901 aatcccgggt tccgattcca cggcgatgct gccgccgaga ttttctacct gttgtttgac
      961 aagtgctaaa ccgacgcctc gctcgcttcc ttttgtcgag acacctttgt caaaaatgtg
     1021 atcgattttg tcgggtgcga ttcccggtcc atcatcgtta acttcgcagt gcagccagcc
     1081 gtgacggtag tgcaatgtta cgctaatttc gccaccgggt tccggcccta atgcttccag
     1141 cgcgttttct atcagatttc ccaatgtggt aatcagcgtc gcgacctggt cctcactgcc
     1201 gctgtctggc agctggcttt cactgtttaa aatcagcgta tggcctaaat cggtcgcgcg
     1261 gttaatcttg ctgattaaaa aaccagcgat aaccggagat ttgatcttac ccagcagaga
     1321 gccaatctct tcctgatagt tattggctgt tttgagaatg taatcttcca actgcttata
     1381 actcttcaga tgcaataatc cgagaatcac atgcaattta ttcataaatt cgtgggatcg
     1441 ttcacgaagt gcatcagcat agttgaccag accgtcgagt cgctgcatca gtttacgtac
     1501 ttcagttttg tccctgaagg ttgaaatggc accgatgata acgccattac tgcgcaccgg
     1561 aacggtgttg atcagtaata gccggtcttt aatcgtaatc tcttcgtcgc ggcgcggcgt
     1621 accgtcgcgt aacacttccg agacatctac cacctgtgac catgagtggc tgagcgtcga
     1681 cagtttctca tcgtcctgcg acttacggta attcagcaat tcttgtgcgg catcgttgat
     1741 cagcgtgacc tcgccgcgat cgtccacggc aacgacgcct tctttgatag actgcaacat
     1801 ggcctggcgc tgctcaaaca gcgtggagat ttcgtagggt tccaggccga aaaggatttt
     1861 tttcagtacc ttaaccagaa tgcaggtgcc aatcagtccg accagcatgc caaataatac
     1921 cgaccagata atgctccagc gactgtcatt gatttgttgg gtcacgcggc ttaactcaag
     1981 gccgatcgcc accacgccaa tttgtttatg attttcatcg tagatggggg taaatacgcg
     2041 taaagcctgc gccagaaaac cgcgattgat agcgacattt tctttgccat tcagcgcttt
     2101 aaggatgtca tcacctttaa atggctgacc aatacgctgg gcttcaggat gcgagtagcg
     2161 aagactttgc atatcggtaa cgacaataaa cagcagatcg ttgcgtttgc gcacggcttc
     2221 cgcgatggcc tggattccgc tctcctgcgg ttttttctgc aagccctgac ggatttccgg
     2281 cgagtcggcg agggtacgcg ccactgccag tgccttgttg gccagcccgt ctcgcgtcat
     2341 atcactgatt tgcgagaagt aaatcagatg caccaccaat agcaccgaga acagtaccgc
     2401 gctgaccatt aagatcactg tggtactcaa tttcatcgga cgtttgcgta acatgcggta
     2461 gggcaatgaa tgtctcatca gcttccttgt gtgacaaatt tcttaagcat tatctctgat
     2521 gaggcgggta attcaaaggg agtaagaatg attggctata taggggaaga gactctggca
     2581 acggaaactg ccagtgctgt atgaagattc cagggctatg cttatagcga taatcatact
     2641 gatgagagag ggaaagtcat ggatcaggcg ctactggacg agggttatcg ctgttatacc
     2701 ggcgaaaaga tcgatgtcta tttcaacact gcgatatgtc agcattctgg caattgcgta
     2761 cgtggcaacg gcaagttatt taatctcaaa cgaaagccgt ggatcatgcc ggatgaagtc
     2821 gacgtcgtca ctgtggttaa agtgattgat acgtgcccga gcggcgcgct gaaataccgt
     2881 cataaataag cgagggtaaa atggaaatac gcgaaggcca caataaattt tacattaatg
     2941 acgaacaagg caagcaaatc gctgaaattg tctttgtgcc gaccggagag aatttagcga
     3001 ttatcgaaca taccgatgtc gatgaaagcc tgaaagggca ggggattggt aaacagctgg
     3061 ttgcgaaagt cgtggaaaaa atgcgtcggg aaaaacgaaa aattatccca ctatgcccat
     3121 ttgcgaaaca cgaatttgat aaaacgcggg agtatgatga tattcgcagt tgatgggaga
     3181 gtacagagtc acgatatttt tcatgctctc cgcggagtga tgcaggagag catctgaagg
     3241 gtagggggat gcacaaagaa tgggcagaga gcgcgttttt ttgtcccaag tcatcccctt
     3301 tactgagcaa aaaaaagaat atctcctata tgagaatcat caatcggggt taataatttt
     3361 tgcgtcccca gagcattcaa tattgatagg agtcatatta tggaaggtaa aaacaagttc
     3421 aatacttatg ttgtttcttt tgattatcca tcatcttatt cctcagtgtt cttaaggtta
     3481 agatcattga tgtatgatat gaatttctct tctatcgtgg ctgatgaata tgggatacca
     3541 cgacaattga atgaaaattc cttcgcaata acgacatcgt tagccgcaag tgaaatcgaa
     3601 gatttaatca ggcttaaatg cttagactta ccggatattg attttgacct caacattatg
     3661 acagttgatg actatttccg tcagttttac aagtagccag gaaggataag aagaacataa
     3721 aaaatggcac tattctctaa aatattaatt ttttatgtga ttggtgtgaa catatccttt
     3781 gtcattatct ggtttatctc acatgagaaa acacatattc gtttacttag tgcattcctg
     3841 gtcggaataa cctggccaat gagtctgcct gtggcattac ttttttctct cttttaggag
     3901 ctgtagttga gtggacccgc tggttatcgt gaaacgaagc gtctctcctc aaaactggat
     3961 ttgcggaacg acgaacgaag cagtcgcctg actgcttcgt tcattaaagt gaaatttatt
     4021 tctgtgggcg catcgccggg aagagaataa cgtcgcgaat agtatgactg tttgtaaaca
     4081 gcataatcat tcggtcgata ccaataccca gaccagcggt tggcggcaga ccatattcca
     4141 gcgcagtcac gtaatcttcg tcatagaaca tggcttcgtc gtcacctgca gctttagcgt
     4201 taacctgttc ctggaaacgt tcagcctgat cttctgcgtc gtttaattcg ctaaaaccat
     4261 taccgatttc acgaccaccg atgaagaatt caaaacggtc ggtgatttct gggttaacat
     4321 cattacggcg tgccagcggt gacacttctg ccggatattc cgtaataaag gttggctgaa
     4381 tcagatgtgc ttctgccact tcatcaaaga tctccgtgac aatacgtccc agcccccagc
     4441 ttttctctac cgtaataccg atagattcag ctaatgcttt agcagcatca aaattatcca
     4501 ggtcggccat atcggtttct ggacgatatt ttttgattgc ttcgcgcatg gtgagttttt
     4561 caaacggttt gccgaaatca aacacatgct cgccataagt gactttagtg gtacccagaa
     4621 cctcttgtgc cagggtgcgg aacagggact ctgtcagttc aatcaaatcg tggtaatccg
     4681 catacgccat gtagagttcc atcattgtga actcaggatt atggcgaaca gaaatacctt
     4741 cattacggaa gttacggttg atttcgaata cccgttcaaa accgcctaca accagacgtt
     4801 tcagatacag ctccggcgcg atacgcaggt acatatctaa atccagagcg ttatgatggg
     4861 taataaacgg gcgagcagat gccccacctg gaattacctg catcatcggg gtttctactt
     4921 ccataaagcc gcgcgcgacc atgaactggc ggatagcggc cagaattttt gaacggacaa
     4981 caaacgtttg acgggattta tcgttagcga tgaggtccag ataacgttga cgataacgga
     5041 cttcctgatc ctgcagacca tggaatttat ctggtaaagg acgtagtgct tttgtcagca
     5101 ggcgcaactc agtacagtga atggaaagct caccggtttg cgtcttaaac agcgtaccgc
     5161 gggcaccgat aatgtcaccc agatcccatt ttttaaactg atcgttataa acaccttctg
     5221 gcaggctatc tcttgcaacg tacagttgaa tacggccacc gacatcctgc aacgttacaa
     5281 aggaggcttt ccccatgata cgacgggtca tcattcggcc agcaaccgag acttcaatgt
     5341 ttaaggattc cagttcctgg ttatccttcg catcaaactc ttcgtgcagc tggtcagagg
     5401 tatggtcgcg gcgaaaatca ttgggaaacg ccacaccttg ctgacgtagt gccgccagtt
     5461 tttcgcggcg atttctcagt tcatcgttaa aatcaatagc ctcattggct ccccgtgttt
     5521 cttgttcaga cattttggtt cctctaaatc cagctttcaa tttagcgtag ataaagagac
     5581 agattggtac actgtattat ctgcctcgac tattaataac tcagtttatc ttatcgaaac
     5641 taacgcttac ctcaagttga tatcactaat aaataaccat caaaatggtt taaatgactt
     5701 attaatctag atttgctagt gttataaact gaaagttaat ttattcataa ccaattgttt
     5761 ttacgacagt taatcgttgc tctcctgtat catattcgtc ggcgtgctaa acagaaaacg
     5821 ggtagcaaag gccaatacga cgatgatcgc gacacaagcc aacgtccact ctcccatctg
     5881 agaaaagaat cgctggtaag ctgcaattgc cataccgcta atttgcgact ctgtcgtctg
     5941 ctgtgccacg acgcctgcca gccagttggc gaccgcgcct gttgccagca tataaatacc
     6001 ggttaatacg ccagacattt tcagacgcgt gatttgcgca atcgccaccg ggtcaataaa
     6061 gagttcggca aagcccatta gcgccagccc ggatatcatc acggccattg acgcttgacc
     6121 gccagccgct gcatgtcggg catcaaatgc caacaacata aagccacaag ccatcagcag
     6181 taagccaaag gcaaacttca gccagacgcg caatgttgag ttgccgcggc tttctggcga
     6241 cgccagccag gccagtacaa ccccagcgag catcactgca attgcattca ccgactggaa
     6301 tagtgctgta ggtacttcaa tattgaatgc ctgacgattc acaaagcgat cgataaacaa
     6361 gctgatggta ctgccgccct gttgtgccag tacccagaac aacgtcccga caaacatcaa
     6421 caatacaatt tgccaaagag cacggcggtg ttcggggaat ttgatcatca tgcgggcaat
     6481 gatttgtgcg gcaatgaggc aaacgatcgc cagcaaatat ccggaccagt cgttctccag
     6541 cagcagggta aaaaatactg gggctaaaca gagcatcacc actaaccagc tccatactgg
     6601 taaggcaaat ttcacgctag tgagcgcttt tttatccata ctacgcgtgg actggaaatg
     6661 acgatgcccg cttaagaaaa tcaacaaacc gataaacatg ccgccacccg caagggcaaa
     6721 gccaacatgc catccatacc actgagcagc caggccgcag gcgattgggg cggcgataga
     6781 accgatattg cccgcagcat acagcagcga aaaaccgcca tcacgtcgat gatcgttctc
     6841 gtcgtagagc tcgccaagca aacagctgat gtttgatttg aataaaccgt agccacaaat
     6901 aatgattgcc agcgccagat acaggctaaa ggttgaattt gtatcaatgc ccagcaccac
     6961 atggccaagg gtcattaaca gcgcgccggc aatcactgca gtgcggttgc cgagcaggcg
     7021 gtcggcaagc cagccgccga gaataggggt aacgtaaacc agagaagcat atgcgctgaa
     7081 caggctgatg gcatggttat catcaaaacc aagctgatgg gtgagataga gaatgagtaa
     7141 ggcacgcatg ccgtaaaaac tgaagtactc ccagatttgg atcgccacga tatagtatat
     7201 cgcgcgcggc tgtgagggtg ttttcatgtg ttctccttat gagcaaaaaa gggaagtggc
     7261 aagccacttc ccttgtacga gctaattatt ttttgctttc ttctttcaat accttaacgg
     7321 tatagcggcc atcagcctga cggtatgcac cgtgaatatc ggtttcaaag cccggatagt
     7381 gagcgccgat ttcacacagc atctgcagga actccagaac cggacggctt tcttcggtga
     7441 tcatttcacc cggcattacc agaggaactc ccggcggata cggaaggatc atattggcgt
     7501 taatacgacc taccatttcg tcgaggtaaa cttcttcggt cataccgtgc agctctttct
     7561 ggaatgcagc atacggagtc attaccatcg tcggcagcac ttcaaatgcg cggtacatca
     7621 gatccggcag attgtggtga acaatcagtt tgtggatatt ctgagccagt tcctgaatac
     7681 gcatgttttc atagaattca ggatcttcac gatacagaga cggcagcatg tttttcacac
     7741 gcaggttcag gtcgaacgca cgtttgaagt cagtcagagc acgcagcagg ctcagtgctt
     7801 tggtcttatc gataccgatg ctgaacagga acagcaggtt atacggaccg gttttctcaa
     7861 caacgatgcc atgttcgtcg aggtatttcg ccacgatgct ggccggaata ccaaagtcgc
     7921 tcatggtgcc gtctttttcc atccccggag tcagcagggt gactttgatc gggtcaagat
     7981 acatgtgctc gttatcgatg tttttgaagc cgtgccaggt gctgtcagaa cgcagcggcc
     8041 agcattcagt cgtatcgata tgatccggct gccatacatc aaagaaccag ccatcagatt
     8101 ccgttctcag acgtttgatc tctttacgga atttgatcgc acgttcaatg gaaccgttga
     8161 tcagacgttt acctgcattg cctttcatca tcgccgcagc ggtttcagtg gacgccacga
     8221 taccgtagtg cggagaagtg gtggtgtgca tcatgtaggc ttcgttaaag gtttcttcgt
     8281 ttacgtcacc tttaacgtgg atcatggaag cctgagagaa cgccgccagc agtttgtgag
     8341 tggactgggt ttcgtaaatc actttccctt ctacacggcc accgctcata ccgcatttac
     8401 cttcgtaaat cggtgagaag ttggtgtaag gcacccacgc ggagtcaaag tggatggatt
     8461 tcacatccag tgttttcttg atgaagtcgg tgttgtacag cagaccatca taggtagagt
     8521 tggtaattac agcatgtacc ggccaggttg cgtttggtgt ttctttcacg cgcttagcaa
     8581 tggtagcgtg ctggaattca ctctgtggga taccaccaag aataccgtaa gcgttacggg
     8641 tcgggcggaa atagattggc gtaacatcgc tcatcatcat caggtgggtc agcgatttgt
     8701 ggcagttacg gtcaatcaga atggtgctgc ctgccggagc agagtacatg ccaacaattt
     8761 tgttcgcagt ggaagtaccg ttggtcacca tgtagctgcg gtctgcgtta aagacgcgag
     8821 cgatatactg ttctgcttct ttgtgtggac cactgtgatc cagcagagaa cccagttcag
     8881 atactgaaat ggaaatatca gatttcatgg tattcggacc aaagaaatca tagaacaggc
     8941 tacctaccgg gcttttctgg aatgcagtac cgcccatgtg accaggagta cagaaagtat
     9001 atttaccttc acgaacatat ttaaacagtg ctttagtcag cggaggcaga atagtgttga
     9061 tatattcgtc agtggtctgc ttgatcttat tagcaatatc ttcagcagca cccagcgcat
     9121 attcaaagaa gctaatctgt aaacgcaggt cattcaggct tacatcgaga gtggaatacg
     9181 tattagcgaa cgcgtacaac ggcaggttct cgttcatttt gctaatttct tcgcacagct
     9241 cgagattata tttatcccag tcaaaaataa cgccgcacag acgcgcattg ttttcgatca
     9301 gttttaataa gtcgtcacgg tcgttcgggt aaacaatctg gaagttcaga cgttcaagcg
     9361 cgcgatgaag ttcacggatg ggttcttctt taaaataaac ccccatgtga ttcaatattg
     9421 caataacgtt catagtcata tctccaggta aaaaaggccc ctcccaacac atgggacaaa
     9481 atgaaaggag gagcctcgga aaatactttt aattaatgtg cgttagacgc ggtgtggtta
     9541 tccattgagt ggctctggcg ctcgtgcatt ttgcgagcgt agaacatcag gataatcagg
     9601 ctgacgatga aggtacctgc cagctcgaag gagcttgcgc ccatcagcgc gatgaagcag
     9661 aacacacaac ccagtacaga gcagatcagg ctgacaaagt tgcggatgtt aacgccttca
     9721 aaacgaatca ggtcaacgca agagtagaag tacggcagca tagtcagcag tactgcgata
     9781 ccggtcagtt caccgaacag gtcagatgct ttaccaccgg cagagttcat cagagtgatg
     9841 aggatcatca gggcagtcat tttcactgca gccagcagca gacctttttt cggaataccg
     9901 ttgctgtcga cttcaccata aactttcggg aagttaccgt cgttagcggc acgtacacct
     9961 gcctggccta ccaacatcat ccaggagccc agagaagtca ggcacgcaaa ggcggtgaat
    10021 gcagaaacca gcggcgcagc ccagttaccg aggatggttg aagcactgat tgcaaacgga
    10081 gcaccggaag ctgccattac agaagatgga tacataccgg aaagcacctg agtcgcagcg
    10141 atgtaaacaa tacctgctaa accagtaccc agcatggttg ccagcggaac ggtacgtttc
    10201 gggtttttaa ccataccagt acttactgct gcggattcaa cacccacgaa ggcccacagg
    10261 cagagcagaa tacttttaat gatcgcatga ccatcagtgg tatccgcagt attccagtta
    10321 gctgcataag ttgccgcatc aaaccaatgc cagccaacaa tagcggtcat tactaccgga
    10381 ataagaacca gcaccagacc aatagtggtt aaacggctta cccaagtacc gccgagcata
    10441 tttacaaagg taaataccca gacgatagca atacaggcga tacccgccgg aacaggatca
    10501 tttaatactg ggaagaaggt ggaaagataa gataccgcgg taataccaat cgccaggtta
    10561 ccaatccagt tagcatggta ataaagaaca cctgtctgaa aaccaaatgc aggggaaatt
    10621 tctccggcat aagcaattgg gccaccttgt tgcgggtttt ttgttgccag tcgggcatat
    10681 acatacgcca gcgacattgc accaataata gagataatcc aaccccagat agcaatacca
    10741 ccgatacttg ctaggttcgc aggtaataat gcaataccgc tccccatcat attaccggca
    10801 acaacaccgg tacaggcaaa tagcccgatc ttcttggcag aactcatgct cttctcctaa
    10861 tttcattttt gaatttggag tccgggtcat gatgtataac tatttcctga ccagaccaaa
    10921 ctggcgctaa gaatactcac gaaaaaagga ttaatcctaa agattaggtg aaataacaca
    10981 aaagtttctg taagtgagaa cttgaggttt tttattaaca catcaggatc gcaagttgat
    11041 atcatg
//
LOCUS       DAZGJW010000143        11025 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZGJW010000143 DAZGJW010000000
VERSION     DAZGJW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.143"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(145..2412)
                     /gene="adiA"
                     /locus_tag="ACHKIK_003996"
     CDS             complement(145..2412)
                     /gene="adiA"
                     /locus_tag="ACHKIK_003996"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO9085070.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2611..3519)
                     /gene="melR"
                     /locus_tag="ACHKIK_003997"
     CDS             complement(2611..3519)
                     /gene="melR"
                     /locus_tag="ACHKIK_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO9085071.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCKSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRAMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3802..5157
                     /gene="melA"
                     /locus_tag="ACHKIK_003998"
     CDS             3802..5157
                     /gene="melA"
                     /locus_tag="ACHKIK_003998"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO9085072.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALHDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5260..6681
                     /gene="melB"
                     /locus_tag="ACHKIK_003999"
     CDS             5260..6681
                     /gene="melB"
                     /locus_tag="ACHKIK_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO9085073.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6820..7449)
                     /gene="yjdF"
                     /locus_tag="ACHKIK_004000"
     CDS             complement(6820..7449)
                     /gene="yjdF"
                     /locus_tag="ACHKIK_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO9085074.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7572..9218)
                     /gene="fumB"
                     /locus_tag="ACHKIK_004001"
     CDS             complement(7572..9218)
                     /gene="fumB"
                     /locus_tag="ACHKIK_004001"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO9085075.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9296..10636)
                     /gene="dcuB"
                     /locus_tag="ACHKIK_004002"
     CDS             complement(9296..10636)
                     /gene="dcuB"
                     /locus_tag="ACHKIK_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO9085076.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
BASE COUNT         2591 a         2687 c         2913 g         2834 t
ORIGIN      
        1 aaaaaaaagc gcggggtggg gccgcgcaaa aagtatgaca tttttgtttg cagcaatttt
       61 tatgaaaacg ttgccatact caaaaggcct aaggccaggc ggcgtaatgt tatttaaaca
      121 attacgcctt cagcggaata gtggttacgc tttcacgcac ataacgtggt aaataccgtc
      181 aataatttca gtcccttcag tttcgtgttc aaatccaggg aaatggtggt cccaggattg
      241 cagcgagcgt aaataactta cttgcggact gtttttatcg ccgaagtttt caccagacag
      301 cagcatcggg attcctggcg gatacgggat aactgagttt gccgcgatgc gtcctggcag
      361 attttcaatg gataccagtt cgacattgtt gtcgacaatc gcgttgtacg cttcacgcgg
      421 ggtgatttcc gccatcggca ggccggaata ggcttcgttc aaccgtgcgc cagggttgtt
      481 ttctttcagc caggcaaaca tggtgtcacc cagatcgtga atccccatgt tcgcgtaagt
      541 gtcaggatat tgttcaacaa gttccggcat cacctgcgcc agcggtgtgt tggcgtcata
      601 gtggcgtttg aaggaacaaa gggtgttaac cagagtcccc catttcccac gggttacgcc
      661 catagagaac aggaacataa tttggaagtc agtggtgcgg gtaggcacaa tgccgtggcg
      721 accaagccag gcagtgacca gcgccgccgg aacaccggtt tcttccagtt caccatcttc
      781 acccattccc ggagcaagga tgctgacttt aattgggtcg agcatactcc agttatccgg
      841 aatatctttg aagccgtgcc agctttcgcc cggatgcatt acccagcagt cctgaacggt
      901 ggtcagcagt ttggttggtg cgtcagcaaa gtcataggtt ttgccggttt gtgggtcggt
      961 gacgacttct ttgttccacg gtttgaagaa ccagctaccg tcagcggtga actctttata
     1021 tagccgcgcc atcgcctgac ggaaatcaac cgcttcgtca atcacttcct gtgtcagtga
     1081 caggccgctg ttgccgtcca tcatcgacac cgccacgtcg ttggatgcgc agatggcata
     1141 cagcggggag gtggtggcat gcatcatgta ggcctggttg aagcgggaga agttaatcgc
     1201 cccacgacct tcacgtacat gaatataaga agcctgtgac agcgcattca gcagtttgtg
     1261 ggtggagtgg gtggcgaaaa cggtaggacc gttgtgatcg ccaggttcgc cgcgcatggc
     1321 atagtgatcg gcatagatcg ggttgaaacg tgcatagccg taccaggctt cgtcaaagtg
     1381 cagacgatcg gaggtttttt ccagcagatc ctgcgcttct ttagcgttat aacacacgcc
     1441 gtcataggtg cagttggtca ccacgcagta agacggtttt tgcccggctt tgtctttggt
     1501 cagcgggctt tcactgattt tcttctgcaa ggtttcaggt tgcatttcct gcggatagat
     1561 tggcccgata atgccgtagc ggttgcggct tggcaccata tagaccggtt tcgcgcctgt
     1621 cagcatcaag ccttgttcga tggatttatg gcaattacgg tcaacgacca cgacatcgtt
     1681 atcggtcatg caagcctgca tgatggtgcg gttagagccg gaagtaccga cgactaccga
     1741 ccaggagcga tcggcaccaa atacgcgtgc ggcatatttt tcgctttcgc caaatgcgcc
     1801 agtatggtca agcaaagaac cgagggaagt tcgttcgatg cccatgtcgg tgcggaacag
     1861 attttcacca tagtagtcat ggtagaaacg tccggcgggt gttttggtaa aaccaacgcc
     1921 gccctggtgg cctggcgctg cccaggaata ttcatggatg tcactatatt tcatcagcgc
     1981 gctgaacagt ggcggcaaca gctgctggcg gtagcgggtc atcgcggcaa cggcgcgtcc
     2041 ggcgataaag tcggcggtat cttccagaat ccaggcgaat tcatcgacaa gctccagcag
     2101 atcccggtcc attgcggcga gggctttttc ccgatcgccc aacaggaaga ccggcacgtt
     2161 ttgttggcgc tcatgaagct taccgatcaa ttgtctgacg ttttgatgtt cgtccggatg
     2221 ttccatttga tagctgaaca tcaggcagtc aatggcttcg tttgaagaga gaatggcaaa
     2281 accatcatca aaggaggtgg atttaatcac ggtaacattt tgctggctta aagcatctgc
     2341 cagacgctca acggcgttac cgacccaggt gtcttgatgg agaaactcgc tttcaacaat
     2401 taatactttc atcattgctt acccggttat gaaggaatct tcgttgcggg caagtatctt
     2461 ccggcctttt cgggctgtaa agcgcgtgaa aaacaaacat ttttgaaata tttagtttta
     2521 taaacaatac gttatgtgat tattttaaat tattttcact gcttattatc gacggctaaa
     2581 ctattttttt ggctgatact gatatcgtct ttagccggga aacgtctggc ggcgctgttg
     2641 gctaagtttg cggtattgtt gcggcgacat gccgacatat ttgccgaacg tgctgtaaaa
     2701 acgactactt gaacgaaagc ctgccgtcag ggcaatatcg agaatacttt tatcggtatc
     2761 gctcagtaac gcgcgaacgt ggttgatgcg catcgcggta atgtactgtt tcatcgtcaa
     2821 ttgcatggcc cgctggaata tccccattgc atagttggcg ttaagtttga cgtgctcagc
     2881 cacatcgttg atggtcagcg cctgatcata gttttcggca ataaagccca gcatctggct
     2941 aacataaaat tgcgcatggc gcgagacgct gtttttgtgt gtgcgcgagg ttttattgac
     3001 cagaatcggt tcccagccag agaggctaaa tcgcttgagc atcaggccaa tttcatcaat
     3061 ggcgagctgg cgaatttgct cgttcggact gtttaattcc tgctgccagc ggcgcacttc
     3121 aaacgggcta agttgctgtg tggccagtga tttgatcacc atgccgtgag tgacgtggtt
     3181 aatcaggtct ttatccagcg gccaggagag aaacagatgc atcggcagat taaaaatcgc
     3241 catgctctta caggttccgg tatctgttag ttggtgcggt gtacaggccc agaacagcgt
     3301 gatatgaccc tgattgatat tcactttttc attgttgatc aggtattcca catcgccatc
     3361 gaaaggcaca ttcacttcga cctgaccatg ccagtggctg gtgggcatga tatgcggtgc
     3421 gcgaaactca atctccatcc gctggtattc cgaatacagc gacagcgggc tgcgggtctg
     3481 tttttcgtcg ctgctgcaca taaacgtatc tgtattcatg gatggctctc tttcctggaa
     3541 tatcagaatt atggcaggag tgagggagga tgactgcgag tgggagcacg gttttcaccc
     3601 tcttcccaga gaggcgaggg gagcgtccga gcatcatgag gccgaaaact ctgcttttca
     3661 ggtaacttat taccataaac tcagatttac tgctgcttca cgcaggatct gagtttatgg
     3721 gaatgctcaa cctggaagcc ggaggttttc tgcagattcg cctgccatga tgaagttatt
     3781 caagcaagcc aggagatctg catgatgact gcacccaaaa ttacatttat cggcgctggt
     3841 tcgacgattt tcgttaaaaa tattcttggt gatgtgttcc atcgcgaggc gctgaaaacg
     3901 gcgcatattg ccctgatgga cattgatccc acccgcctgg aagagtcgca tattgtggtg
     3961 cgtaagctta tggattcagc aggggccagc ggcaaaatca cctgccacac ccaacagaaa
     4021 gaagccttac atgatgccga ttttgtcgtg gtggcatttc agattggcgg ttatgaacct
     4081 tgcacggtga ctgatttcga ggtctgtaag cggcatggtc tggaacaaac cattgccgat
     4141 acgttggggc cgggcggtat tatgcgcgcg ctacgtacca ttccgcatct gtggcaaatt
     4201 tgcgaggaca tgacggaagt ctgccccgat gccaccatgc tcaactatgt taacccaatg
     4261 gcgatgaata cctgggcgat gtatgcccgc tatccgcata tcaaacaggt cgggctgtgc
     4321 cattcggtgc agggaacggc ggaagagctg gcgcgtgacc tcaatatcga cccagctacg
     4381 ctgcgttacc gttgcgcagg tatcaaccat atggcgtttt acctggagct ggagcgcaaa
     4441 accgccgacg gcagttatgt gaatctctac ccggaactgc tggcggctta tgaagcaggg
     4501 caggcaccga agccgaatat tcatggcaat actcgctgcc agaatattgt gcgctacgaa
     4561 atgttcaaaa agctgggcta cttcgtcacg gaatcgtcag aacattttgc tgagtacaca
     4621 ccgtggttta ttaagccagg tcgtgaggat ttgattgagc gttataaagt accgctggat
     4681 gagtacccga aacgctgcgt cgagcagctg gcgaactggc ataaagagct ggaggagtat
     4741 aaaaacgcct cccggattga tattaaaccg tcacgggaat atgccagcac aatcatgaac
     4801 gctatctgga ctggcgagcc gagtgtgatt tacggcaacg tccgtaacga tggtttgatt
     4861 gataacctgc cacaaggatg ttgcgtggaa gtagcctgtc tggttgatgc taatggcatt
     4921 cagccgacca aagtcggtac gctaccttcg catctggccg ccctgatgca aaccaacatc
     4981 aacgtacaga cgctgctgac cgaagctatt cttacggaaa atcgcgaccg tgtttaccac
     5041 gccgcgatga tggacccgca tactgctgcc gtgctgggca ttgatgaaat atatgctctt
     5101 gttgacgacc tgattgccgc ccacggcgac tggctgccag gctggttgca ccgttaaaac
     5161 gcgactaaac gctactgcgc cgggggattt attccggcgc acacctctga cgataccaat
     5221 aacagaaggc gggcgttggt aacagcgacc cgatacccta tgagcatttc aatgactaca
     5281 aaactcagtt atggatttgg agcgttcggg aaggattttg cgatcggcat tgtgtatatg
     5341 tacctcatgt attactacac cgatgtcgtt gggctgtctg tgggtttggt cggtactttg
     5401 tttctggtgg cgaggatctg ggatgctatt aacgatccga ttatgggatg gattgtaaat
     5461 gctacgcgat cgcgatgggg taagttcaaa ccctggatcc tgatcggtac gttggcaaac
     5521 tctgtaatct tatttctcct ctttagtgcg catctgtttg aaggtactac tcagattgtc
     5581 tttgtttgcg tgacctacat cctctggggc atgacttaca ccattatgga tattcccttc
     5641 tggtcgctgg ttccaaccat cacgctcgat aaacgtgagc gcgaacaact ggttccttat
     5701 ccgcgttttt ttgccagtct ggcaggcttt gttacggcag gtgtgacgct accatttgtt
     5761 aattatgtcg gcggtggcga tcggggattt ggctttcaga tgttcactct ggtactaatc
     5821 gcctttttta ttgtttcaac catcatcact ctgcgcaatg tgcatgaagt cttttcgtca
     5881 gacaatcaac cgtctgctga aggaagccat ctgacactta aagccatcgt tgcgctaatt
     5941 tataaaaacg atcagctttc atgcctcttg ggtatggctc ttgcttataa tgtagccagc
     6001 aacattatta ccggatttgc tatctattat ttctcatatg ttatcggtga tgcggatttg
     6061 ttcccctatt atctgtcgta tgcgggagct gctaacctgg tgacgttagt attcttccca
     6121 cgcttagtta aatcattatc ccgacgcatt ttatgggccg gagcatctat tcttccggtg
     6181 ttaagctgtg gtgttctcct gttaatggca ttaatgagct atcacaacgt cgtcctcatt
     6241 gtgattgcgg gtattttgct gaatgtggga acggcgcttt tctgggtatt acaggtcatc
     6301 atggtggcag ataccgttga ttacggtgaa tataaactgc acgtacgctg tgaaagtatc
     6361 gcttactccg tgcagactat ggtggtgaag ggcggttcag cctttgcggc ttttttcatt
     6421 gcggttgtgt tagggatgat tggctatgta ccgaatgttg aacagtctac gcaagcccta
     6481 ttaggtatgc agtttattat gattgctcta ccaactctgt ttttcatggt aacgctgatt
     6541 ctctacttcc gtttctatcg cctcaatggc gacacgctgc gcaggatcca gattcatctg
     6601 ctggataaat atcgcaaagt accgcccgag cctgttcatg ctgatattcc ggtcggtgca
     6661 gtgagtgatg tgaaagcctg acgtggacaa tagctaacga aaatggcctg atgcgatatg
     6721 tttatcaggc cacgtcttaa tgtaatgctt tggatttgca aattttgtag accggataag
     6781 gaattcacgc cgcatccggc atcaacaaag cgcacgttgt tatccggtta tcaagccaaa
     6841 gcgccgtagc tggcggcaat gaaagcgagc gaggaatatc accgtcgtta atgcgccaag
     6901 cagcgcgcag aacatatcgg attgcgtgtc ccactggtcg ccctgggtgc cgagaaaatc
     6961 atccgctccc tggcccattg ccagcgccgc ccaccactcg attaattcat acatcgcgct
     7021 tatcgccagc gccacgcagc agaccaggaa cgccaccatt ttacgtccgc gcacgtacat
     7081 cccgcgcacg agaatttctc gtgccaccag tgcaggcacc agcccctgga aaaaatgccc
     7141 cagcttgtca tacggattac ggctcaaccc cagccattcc tgcacctcaa aaccgatggg
     7201 gactttcgcg taggtgtatt gcccgccgac catcaggatg atggcgtgaa agaaaatgag
     7261 cgtatagagc agcggcgtta acggataacg tttagcggtg gcaagcagta gcggcacgac
     7321 aataatcacc ggtgtcactt ccatcagcca ggtgagtttg tcatgggccg aaatgccggt
     7381 ataaatcagg attaacgtta gcgccagtgc gccggtgtta agaattaacg gcttgagtgt
     7441 acgggtcatg gtgagttcgc aagtcaggga aaatactgac tattcaccag caagcgtgaa
     7501 attacaatcg caaaagaggt ggcccagggg gatcacctgg cagcatgctg ccaggcgctg
     7561 ggccgaagag gttacttagt gcagttcgcg cactgtttgt tgacgatttg ctggaagaag
     7621 tcgttacctt tgtcatcgac caggataaac gccgggaaat cttctacttc gattttccag
     7681 atagcttcca tacccagctc cggataagcg acgcactcca gatgcttgat gctctgctgc
     7741 gccagtaccg ccgccggacc gccgatgcta ccgaggtaga agccgccgtg tttatgacac
     7801 gcgtcggtaa cctgctgact gcggttacct ttcgccagca tgatcatgct gccgccgtgg
     7861 gattgcagca gatccacgta ggagtccata cggcctgcgg tggttgggcc aagtgaacct
     7921 gatggataac cggcaggggt tttcgccgga cccgcgtagt agatcgggtg atctttgatg
     7981 tactgcggaa gttctttacc ggcgtcaatc agctctttca gcttggcgtg tgcaatatct
     8041 cggcccacga taatggtgcc ggtgagcgac aaacgagtgg ataccgggta ttgcgaaagc
     8101 tgggcgagga tctctttcat cgggcggtta aggtcaactt tcaccgcttc gccttcaccg
     8161 gcctggcgca gttcttgtgg aatgtactgg cctgggttgt gttccagttt ttcgatccag
     8221 ataccttcgc ggttgatttt cgctttaatg ttacggtcag cggagcagga gacgcccatg
     8281 ccgaccgggc aggatgcgcc gtgacgtggc agacggataa cgcgaatgtc gtgcgcgaag
     8341 tatttaccgc caaactgcgc gccaagaccg agtttctggg cctcttccag cagttcctgt
     8401 tccagctgga catcgcggaa cgcctgacca tgttcgttcc cttccgtcgg cagttcatcg
     8461 taatagtgag cgcttgctaa cttgacggtt ttcaggttgg tttccgcaga cgtaccgcca
     8521 atcacaaacg cgatatggta cggcgggcag gctgcagtac cgagggtacg cattttctcg
     8581 acgaggaagt ttttcagttt gccgggagtc agcagggctt tggtttcctg gtagagatac
     8641 gttttgttgg cagagccgcc gcctttcgca acgcaaagga atttgtactc atcgccatct
     8701 accgcgtaca ggtcgatttg cgcaggcagg ttagtgccgg tgttgacctc tttgtacatg
     8761 tccagcgccg cattctgtga atagcgcagg ttatcttcga tataggtgtt atagacgcct
     8821 ttcgacagcg cttcttcatc accgccaccg gtccacacgc gctggccttt tttaccgacg
     8881 atgatcgcgg tgccggtatc ctggcaggtc ggcagcacgc ctttggcggc gatttcggag
     8941 tttcttaaga attgcagcgc cacgtacttg tcgttttcgc tggcttctgg atcgtgaaga
     9001 atagccgcaa cctgtttctg gtgtgccgga cggagcatga aagaagcgtc gtgaaaggct
     9061 tgctgcgcca gtagggtcag ggcttctggt tccactttca ggatggtttc gccgtcgaag
     9121 tcggcaacgc taacgtaatc ggaagtgagt agatagtatt cggtattgtc tttccccatc
     9181 gggaaaggtg cctggtagat aaagggtttg tttgacatag cttccagcct gtaactctgt
     9241 atttgttatt cgaaaatggc gtgccgcgta gtcacgggcg gcacgcaaag tgcatttata
     9301 agaacccgta catcgcggcg aagatccagc cgaagacgca cgatacgctc acaccaatca
     9361 accccggcag aataaaactg tggttgatga cgaagcgacc gatgtgggtg gtgccggaac
     9421 ggtcaaactg aatcgctgcc agatcgctcg gataagtcgg caggatgtaa taaccgtagc
     9481 aagccggtgc tgaagccacg atgtatgccg gatcaacgcc gatcgccagc gcgaccggaa
     9541 caatcgccgc cagcgccgca gcctgagagt ttacaaactt ggaaaccagc agcagaacaa
     9601 tggcatacgc ccacggatac tctttcacca tttcacccag tacgccctga atttcagaca
     9661 tatgcgcacc gaacatggtt tctgccatcc atgcgatacc gtacaccgcc acgatggcga
     9721 tcataccgga acggaagact tcgttttttg agatagacgc gggattggtt ttggtcagga
     9781 taataatcag cgccccggtc agcagcataa acatctgaat aaccagtacc atcgacagcg
     9841 gtttgccgcc gaaggatgga cgcaggtccg aatcagcacc aagaagggcg actacagcga
     9901 ttgcaccgag gaaaatccac attgccagcc agttgctttt cggcagtttt ttatcgagca
     9961 gcgtcgcggt atcaccgtaa acatactcac ggttttccgg tacggagatg aatttctgga
    10021 actcttcgtc tttatccaga tctttaccgc ggaaccagct gaagataccg atcgccagga
    10081 taccgattaa cgtcgatgga atggtgattg ccagcaggtc gaggaactca agatggcgac
    10141 catcaaaggt gacattaccc agcattgcaa ccagagacac gaccgcaacc gacaccggac
    10201 tggcgataat ccccatctgt gcaccgatag aacttgccgc catcggacgt tccggacgga
    10261 tgttgttctt aatggcgacg tcgtagatga tcggcagaat ggtgtaaacc acatgacccg
    10321 taccgcaaag aatggtcagt gtacaggtca caaacggcgc gacaattgag acatatttcg
    10381 ggttgcggcg cagcagcttc tcggcaattt gcagcatgac atcaagaccg cccgaagctt
    10441 gcaaggtcgc cgatgccgcc accaccgcaa tgataaccag catgacatca accggtggtt
    10501 tacctggctg aaggtggaag acgaagacca gaatgaccag accgataccg cctaataaac
    10561 ccagcgcgat accacccttt ctggcaccat aaaacagaca tatcagtatt atgataagtt
    10621 ggatagtaaa taacatgtgt gaaccctcgc gataatccta tttaaatttt tgctgaatag
    10681 atcacagtca cgttctgttt tgtatgaact gttttcagag cctgactaaa tatccgtttg
    10741 gtcgttaccg gctttagcaa atacctcaca gtaaatattg gctgattaaa gcagcggcaa
    10801 atttatgcac tgtgataagc ggctttttca gagagaaaaa ctctctggcg atgggtttat
    10861 agtttgtact gcgcagatgt taatgttttg tttgctgtta tcttattgat atttatcggt
    10921 ctattttttg ttctcagggc agatatcatt aggtttaaag attaatctga tctacccatt
    10981 tgtgggtaaa aatacacata atgcgggtga cataatagtt aatta
//
LOCUS       DAZGJW010000144        11024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZGJW010000144 DAZGJW010000000
VERSION     DAZGJW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.144"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            246..1388
                     /gene="yghO"
                     /locus_tag="ACHKIK_004003"
     CDS             246..1388
                     /gene="yghO"
                     /locus_tag="ACHKIK_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO9085077.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1395..2159)
                     /gene="glcC"
                     /locus_tag="ACHKIK_004004"
     CDS             complement(1395..2159)
                     /gene="glcC"
                     /locus_tag="ACHKIK_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO9085078.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2410..3909
                     /gene="glcD"
                     /locus_tag="ACHKIK_004005"
     CDS             2410..3909
                     /gene="glcD"
                     /locus_tag="ACHKIK_004005"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO9085079.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3909..4961
                     /gene="glcE"
                     /locus_tag="ACHKIK_004006"
     CDS             3909..4961
                     /gene="glcE"
                     /locus_tag="ACHKIK_004006"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO9085080.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAINEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4972..6195
                     /gene="glcF"
                     /locus_tag="ACHKIK_004007"
     CDS             4972..6195
                     /gene="glcF"
                     /locus_tag="ACHKIK_004007"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO9085081.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6200..6604
                     /gene="glcG"
                     /locus_tag="ACHKIK_004008"
     CDS             6200..6604
                     /gene="glcG"
                     /locus_tag="ACHKIK_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO9085082.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6626..8797
                     /gene="glcB"
                     /locus_tag="ACHKIK_004009"
     CDS             6626..8797
                     /gene="glcB"
                     /locus_tag="ACHKIK_004009"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO9085083.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPECVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFQVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDAAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHAMHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9025..9270
                     /gene="traT"
                     /locus_tag="ACHKIK_004010"
                     /pseudo
     CDS             9025..9270
                     /gene="traT"
                     /locus_tag="ACHKIK_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            complement(9303..>11024)
                     /gene="cptA"
                     /locus_tag="ACHKIK_004011"
     CDS             complement(9303..>11024)
                     /gene="cptA"
                     /locus_tag="ACHKIK_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO9085084.1"
                     /translation="HEHVFSWKALGWALIYFWYFSTLLQIIICLTGYSGTNGLRDSLL
                     FSSLWLIPVFLFPERIKVTAALIGVVLWGTSLAALCYYLIYGQEFSQSVLFVMFESNT
                     SEASEYFSQYFSLKILFISTIYTAIAILLWTRLSPIYIPSYWRYIIPLFIIYGLILHP
                     VLINTVVKNRTIDNTLGRLASRMEPAAPWQFITGYYQYRLQLNSLNRLLEENNALPAL
                     KNFNDISGDTPRTLVLVIGESTQRGRMSLYGYPRKTTPGLDELYKTEQNMFVFNNVVS
                     SRPYTIEVLQQVLTFADEQQPELYLTQPSLMNIMKQAGYKTFWITNQQTMTARNTMLT
                     VFSRQTDKQYYMNQQRTQSAREYDTNVLAPFSEVLADPAPKKFVIIHLLGTHIKYKYR
                     YPEGQGVFDGDREHAPEGLTDNELELYNDYDNANIFNDHVVATLIKTFKATAPDGFLF
                     YFSDHGEEVFDTPPHKMLGRNEDTPTRPMYNIPFILWASEKWLLNRHHTFSEYINRKY
                     SLADFIHTWSDLAGLRYDNYEPERSILNQHFKEITRWIGNPYKKNALTDYDTLPYAEL
                     RVNQASN"
BASE COUNT         2761 a         2661 c         2994 g         2608 t
ORIGIN      
        1 gtttaagttt tttgttttta ttgtgaatga tatttaattt atgaatatat taagaagaag
       61 ccttgtccgg tcgccttaaa tcgacaaaag gcattgaggc gcttctgtgt agtctctaac
      121 cttatcataa tcattaataa atactattta ttctttgttt gtattcattg ggttgattat
      181 taaggtgtcg ttcgttgctg ttgcaacctg tttttcaccg ttaattattt gttatccaca
      241 gagatatgga atgcgatctg ctgatgatta aaattgaaaa agtaattaat aaaaatgacc
      301 ttaaggcatt tatcgctttt ccgtcatcac tttatcccga cgatccaaac tggatacccc
      361 ctttattcat agaacgcaac gagcatttgt ctgcgaaaaa tccggggacg gaccatatca
      421 tctggcaggc gtgggtggca aaaaaagcgg ggcagatagt tgggcgtatt accgcgcaaa
      481 tagatacctt gcaccgtgag cgttacggtg aagataccgg tcatttcggc atgattgacg
      541 ccattgatga tccgcaggtt tttgccgcgt tgtttggcgc agcggaagcg tggttgaagt
      601 cacaaggtgc aagtaagatc agcggtcctt tcagcctgaa tatcaatcag gaaagcggat
      661 tactgattga aggttttgac acaccaccct gtgcgatgat gccacacggc aaaccgtggt
      721 atgccgcgca tattgaacaa ctgggttatc acaaaggcat tgatttactg gcgtggtgga
      781 tgcagcgaac cgatctcact ttctctccgg cgctaaaaaa actgatggat caggtgcgca
      841 aaaaggtgac cattcgctgc atcaatcgtc agcggtttgc cgaagaaatg cagatcctgc
      901 gtgagatttt caactctggc tggcagcaca actggggatt tgtgccgttt accgaacatg
      961 aattcgcgac catgggcgat caacttaaat atctggtgcc ggacgatatg atctatatcg
     1021 ctgagattga ttctgcaccc tgcgcgttta ttgtcggctt gccgaacatc aacgaggcga
     1081 ttgccgatct gaacggatcg ctctttccct tcggctgggc aaaattgctg tggcgtttga
     1141 aagtcagcgg tgtgcgaact gcgcgagtac cgctgatggg cgtgcgcgac gagtatcagt
     1201 tcagccgcat cggcccggtg attgcgctgt tattgattga agccttacgc gatccgtttg
     1261 cccgccggaa gatcgatgcg ctggagatgt catggatcct cgaaaccaat acaggcatga
     1321 ataacatgct ggaacgcatt ggggcggagc cgtacaagcg ttaccgattg tatgaaaagc
     1381 agatttaata ccagctaact caggttcatc tccagcggca ccgccgagcg aatcaaatgg
     1441 tggccttcca gctcgatatc gtggagattc tttttcacgg tccgcacatg atcgcgtgct
     1501 gcgcgctggg cgacgtgcgg cagccgctgc aacaccgcgt tgtagatcct cgcatgctgg
     1561 cgatcgatct gctttttttg ctgtggtcga tggtagagat tatttacgct ggcaaacact
     1621 gaattaaaca tcagatcggt caatgattgc agcgtaaaca ccagcacctg attgtgagaa
     1681 gcctgacaaa tggcgagatg gaaagcgtga tccaactgcg catgttcgat cagcgaaatc
     1741 tctttgttgt tctcactggc ggcgagcatt ttttcataac agcgggttat cacaacaaaa
     1801 tcagcctgcg ttcccagcgt tgccgccagc cttgccgatt cgccctccag taatgcgcga
     1861 acgtcgagca gatcgtacag cgttcgcggc tgcgtactga acagatggat cagcggactg
     1921 gtgtcctgca cccgattaag ccgtgcgaca cgagaatcgc gaccctgcgc cgtttcaata
     1981 atcccgcgcc cgcgcagcac ggtcagccct tcacgcagtg cggagcgtga gaagccgagc
     2041 ttttcacaca gtcgacgttc cgagggaagt ggctgaccga ccttcagtac gccgtcgata
     2101 attaaccgtt cgatactctc tgcaaccact tcgcaaatag ggcgacgttc atctttcatt
     2161 cccggacctc gtgcacaggt aggaccaatt tttctgttct taactcgcaa aacacgcaca
     2221 tcacgtaagt gattatgtta catcaattta acattgagtt aaccaaggca aggtcacaga
     2281 gctggaaaaa aaatggtctg accggtagct aaagagatag acgaaaacga aaagcccgct
     2341 taataactgt tcacagaagc agcgcgcaaa aatcagctgc cacacaacac aacaaagcga
     2401 agcctactca tgagcatctt gtacgaagag cgtcttgatg gcgctttacc cgatgtcgac
     2461 cgcacatcgg tactgatggc actgcgtgag catgtccctg gacttgagat cctgcatacc
     2521 gatgaggaga tcattcctta cgagtgtgac gggttgagcg cgtatcgcac gcgtccatta
     2581 ctggttgttc tgcctaagca aatggaacag gtgacagcga ttcttgctgt ctgccatcgc
     2641 ctgcgtgtac cggtggtgac ccgtggtgca ggcaccgggc tttctggtgg cgcgctgccg
     2701 ctggaaaaag gtgtgttgtt ggtgatggcg cgctttaaag agatcctcga cattaacccc
     2761 gttggtcgcc gcgcgcgcgt gcagccaggc gtgcgtaacc tggcgatctc ccaggccgtt
     2821 gcaccgcata atctctacta cgcaccggac ccttcctcac aaatcgcctg ttccattggc
     2881 ggcaacgtgg cagaaaatgc cggcggcgtc cactgcctga aatatggtct gaccgtacat
     2941 aacctgctga aaattgaagt gcaaacgctg gacggcgagg cgctgacgct gggatcggac
     3001 gcgctggatt cacctggttt tgacctgctg gcgctgttca ccggatcgga aggtatgctc
     3061 ggcgtgacca ccgaagtgac ggtaaaactg ctgccgaagc cgcccgtggc gcgggttctg
     3121 ttagccagct ttgactcggt agaaaaagcc ggacttgcag ttggtgacat catcgccaat
     3181 ggcattatcc ccggcgggct ggagatgatg gataacctgt cgatccgcgc ggcggaagat
     3241 tttattcatg ccggttatcc cgtcgacgcc gaagcgattt tgttatgcga gctggacggc
     3301 gtggagtctg acgtacagga agactgcgag cgggttaacg acatcttgtt gaaagcgggc
     3361 gcgactgacg tccgtctggc acaggacgaa gcagagcgcg tacgtttctg ggccggtcgc
     3421 aaaaatgcgt tcccggcggt aggacgtatc tccccggatt actactgcat ggatggcacc
     3481 atcccgcgtc gcgccctgcc tggcgtactg gaaggcattg cccgtttatc gcagcaatat
     3541 gatttacgtg ttgccaacgt ctttcatgcc ggagatggca acatgcaccc gttaatcctt
     3601 ttcgatgcca acgaacccgg tgaatttgcc cgcgcggaag agctgggtgg gaagatcctc
     3661 gaactctgcg ttgaagttgg cggcagcatc agtggggaac atggcatcgg gcgagaaaaa
     3721 atcaatcaaa tgtgcgccca gttcaacagc gatgaaatca cgaccttcca tgcggtcaag
     3781 gcggcgtttg accccgatgg tttgctgaac cctgggaaaa acattcccac gctacaccgc
     3841 tgtgctgaat ttggtgccat gcatgtgcat cacggtcatt tacctttccc tgaactggag
     3901 cgtttctgat gctacgcgag tgtgattaca gccaggcgct gctggagcag gtgaatcagg
     3961 caattagcga taaaacgccg ctggtgattc agggcagcaa tagcaaagcc tttttaggtc
     4021 gccctgtcac cgggcaaacg ctggatgtgc gttgtcatcg cggcattgtt aattacgacc
     4081 cgaccgagct ggtgataacc gcgcgtgccg gaacgccgct ggtggcgatt gaagcggcgc
     4141 tggagagcgc cgggcaaatg ctcccctgcg agccgccgca ttatggtgaa gaagccacct
     4201 ggggcgggat ggtcgcctgc gggctggcgg ggccgcgtcg cccgtggagc ggttcggtcc
     4261 gcgattttgt cctcggcacg cgcatcatta ccggcgctgg aaaacatctg cgttttggtg
     4321 gcgaagtgat gaaaaacgtt gccggatacg atctctcacg gttaatggcc ggaagctacg
     4381 gttgtcttgg cgtgctcact gaaatctcga tgaaagtgtt accgcgaccg cgcgcctccc
     4441 tgagcctgcg tcgggaaatc agcctgcaag aagccattaa cgaaatcgcc cagtggcaac
     4501 tccagccgct acccattagt ggcttatgtt acttcgacaa tgcgttgtgg atccgccttg
     4561 agggcggcga aggatcggta aaagcggcgc gtgaactgct ggggggcgaa gaggttgcgg
     4621 gtcagttctg gcagcaattg cgtgaacaac aactgccgtt cttctcgtta ccaggtacct
     4681 tatggcgcat ttcattaccc agcgatgcgc cgatgatgga tttacccggc gagcaactga
     4741 tcgactgggg cggagcgtta cgctggctga aatcgacagc cgaggacaat caaatccatc
     4801 gcatcgcccg caacgctggc ggtcatgcga cccgctttag tgccggagat ggtggctttg
     4861 ccccgctatc ggctccttta ttccgctatc accagcagct taaacagcag ctcgaccctt
     4921 gcggcgtgtt taaccccggt cgcatgtacg cggaactttg aggagcaggc tatgcaaacc
     4981 caattaactg aagagatgcg gcagaacgcg cgcgcgctgg aagccgacag catcctgcgc
     5041 gcctgtgttc actgcggatt ttgtaccgca acctgcccaa cctatcagct tctgggcgat
     5101 gaactggacg ggccgcgcgg gcgcatctat ctgattaaac aggtgctgga aggcaacgaa
     5161 gtcacgctta aaacacagga gcatctcgat cgctgcctca cttgccgcaa ttgtgaaacc
     5221 acctgtcctt ctggtgtgcg ctatcacaat ttgctggata tcgggcgtga tattgtcgag
     5281 cagaaagtga aacgcccact gccggagcga atgctgcgcg aaggattgcg ccaggtagtg
     5341 ccgcgtccgg cggtcttccg tgcgctgacg caggtagggc tggtgctgcg accgttttta
     5401 ccggaacagg tcagagcaaa actgcctgct gaaacggtga aagctaaacc gcgtccgccg
     5461 ctgcgccata agcgtcgggt tttaatgctg gaaggctgcg cccagcctac gctttcgccc
     5521 aacaccaacg cggcaactgc gcgagtgctg gatcgtctgg ggatcagcgt catgtcagct
     5581 aacgaagcag gctgttgtgg cgcggtggac tatcatctta atgcgcagga gaaagggctg
     5641 gcacgggcgc gcaataatat tgatgcctgg tggcccgcga ttgaagcagg tgccgaggca
     5701 attttgcaaa ccgccagcgg ttgcggcgcg tttgtcaaag agtacgggca gatgctgaaa
     5761 aacgatgcgt tatatgccga taaagcgcgt caagtcagtg aactggcggt cgatttagtc
     5821 gagcttctgc gcgaggaacc gctggaaaaa ctggcagttc gcggcgataa aaaactggcc
     5881 ttccactgcc cgtgtaccct acaacatgcg caaaagctga acggcgaagt ggaaaaagtg
     5941 ttgcttcgtc ttggatttac cttaacggac gttcccgaca gccatctgtg ctgcggttca
     6001 gcgggaacat atgcgttaac gcatcccgat ctggcacgcc agctgcggga taacaaaatg
     6061 aatgcgctgg aaagcggcaa accggaaatg atcgtcaccg ccaacattgg ttgccagacg
     6121 catctggcga gcgccggtcg tacctcagtg cgtcactgga ttgaaattgt agaacaagcc
     6181 cttgaaaagg aataaaaaaa tgaaaactaa agtcattctt agccagcaaa tggcgagtgc
     6241 aattattgcc gcaggtcagg aagaggcgca gaaaaataac tggtcggttt ccattgctgt
     6301 tgccgatgac ggcggtcatc tgctggcgtt aagtcgcatg gacgattgcg cgccgattgc
     6361 ggcttacatc tcccaggaga aagcgcgtac cgccgcgctg gggcgtcgtg aaactaaggg
     6421 ctatgaagag atggtgaaca acggacgtac cgcgttcgtg actgcgccgt tactgacgtc
     6481 gctggaaggc ggcgtaccgg ttgttgtgga tgggcaaatt attggtgccg tgggcgtttc
     6541 tggtttaacc ggagcacagg atgcgcaggt cgcgaaagcg gcagcagcgg tgttggcgaa
     6601 ataagcgaaa acgaggagag aaacaatgag tcaaaccata acccagggcc gtttacgcat
     6661 tgacgccaat tttaaacgtt ttgtggatga agaagtttta ccgggaacag ggctggacgc
     6721 tgcggcgttc tggcgcaatt ttgatgagat cgttcacgat ctggcaccag aaaatcgtca
     6781 gttgctggca gaacgcgatc gcattcaggc agcgcttgat gagtggcatc gcagcaatcc
     6841 ggggccggta aaagataaag cggcctataa atctttcctg cgtgaactgg gctacctggt
     6901 gccgcaaccg gagtgcgtga cggtggaaac cacgggcatt gacagcgaaa tcaccagtca
     6961 ggcagggcca cagctggtgg ttccggcaat gaacgcccgc tacgcgctga acgcggcgaa
     7021 cgctcgctgg ggctcactgt acgatgcgtt atacggcagc gacatcatcc cgcaggaagg
     7081 ggcgatggtc agcggctacg atccgcagcg cggtgagcag gttatcgcct gggttcggcg
     7141 tttcctcgat gaatctctac cgctggaaaa cggcagctat caggatgtgg tggcgtttca
     7201 ggtggtcgat aaacaattac gcatccagtt gaaaaatggt aaagaaacca cgttacgtac
     7261 cccggcgcag tttgtcggtt accgtggcga tgccgctgcg ccgacctgca ttttgctgaa
     7321 aaataacggc ctgcatattg aactgcaaat tgatgccaac ggacggattg ggaaagacga
     7381 tgcggcgcac atcaacgatg ttatcgtcga agctgcaatc agtaccattc tcgactgcga
     7441 agattcggtc gcggcggttg atgcggaaga taaaatcctg ctgtaccgca atctgctggg
     7501 cctgatgcag gggactctcc aagagaaaat ggagaaaaac ggtcgacaaa tcgtgcgtaa
     7561 actgaatgac gatcgtcagt acaccgccgc cgatggctct gaaatttctc tgcacggacg
     7621 ctcgctgctg tttatccgca acgtgggtca tttgatgacc attcctgtga tttgggacag
     7681 cgaaggcaat gaaatcccgg aaggcattct tgatggcgtc atgactggcg cgattgccct
     7741 ctatgattta aaagtgcaga aaaactcgcg cactggcagc gtctatattg tgaaaccgaa
     7801 aatgcacggt ccgcaggaag tggcgttcgc caacaaactg tttacccgca ttgagacaat
     7861 gctcggtatg gcaccgaata ccctgaaaat gggcattatg gatgaagaac gtcggacctc
     7921 gctgaacttg cgtagctgta tcgctcaggc acgcaaccgc gtggcgttca tcaataccgg
     7981 tttcctcgac cgtaccggcg atgaaatgca ttcggtgatg gaagctggcc cgatgctgcg
     8041 taaaaatcag atgaaatcga cgccgtggat caaagcctac gagcgtaata acgtgctttc
     8101 cggcctgttc tgtgggctgc gcggtaaagc gcaaatcggt aaaggcatgt gggcaatgcc
     8161 ggacctgatg gcagacatgt acagccagaa gggcgaccaa ctgcgtgccg gggcaaacac
     8221 agcctgggtt ccgtcgccaa ctgccgctac gctccatgcg atgcactacc accaaaccaa
     8281 cgtacagagc gtgcaagcca acattgccca gactgagttc aatgctgaat ttgaaccgct
     8341 gctggacgat ctgctgacta ttccggttgc tgaaaacgct aactggtcgg cgcaagagat
     8401 ccaacaagag ctggataaca acgtgcaggg gattctgggg tacgtggtgc gctgggtgga
     8461 gcaggggatt ggttgttcaa aagtgccgga tattcacaat gtggcgctga tggaagaccg
     8521 cgcaacgctg cgtatctcca gccagcatat cgccaactgg ttacgtcacg gtattctgac
     8581 caaagaacag gtgcaggcgt cgctggagaa tatggcgaaa gtggttgatc agcaaaacgc
     8641 tgacgatccg gcttatcgtc cgatggcggg gaatttcgct aactcgtgtg cttttaaagc
     8701 tgccagcgat ttaatcttcc tcggcgtgaa acagccaaac ggctataccg aaccgttatt
     8761 acacgcctgg cgtttacgcg aaaaagaaag tcattaatag cgtcaaaaaa agccccggca
     8821 agttttattg tcggggctat gatagtaact gcggtagtac atttatcccg gaaataaccg
     8881 gagcgattat cagggtttaa caatattaaa tacccgtagc gcattactag cgggcctata
     8941 acaacaatat ggacatctta acgatactct gcgcctgcgt gtgcagaaca gaaaccctta
     9001 cccgatatac ttgttatgcc agaggtagca attgcggcaa ccgatctgac tggcatggat
     9061 gcagatgcaa tggtggaaga tataaattac acgataatta cagatgttca gattaccgaa
     9121 cgaactaaaa cctatgtcag agcagataat gctaccactt ttcggcaggg aacatcaggc
     9181 accgaaattc aggctagcac agaaaccagc aaccaggtta atctgaaatt tgaaggggct
     9241 aaacctcttc tggaaataca actgaaataa atatcctccg gctatgccgg aggatattta
     9301 ttttaatttg aagcctgatt tacacgcagc tctgcgtagg gcagggtatc atagtcagtt
     9361 aacgcatttt tcttataggg gtttcctatc cagcgagtga tttctttgaa atgctggttt
     9421 aatatggaac gctctggctc atagttatca taacgtaaac cagccaaatc cgaccaggta
     9481 tggataaaat cagccagact gtatttacgg ttaatgtact cagaaaatgt atgatgtcga
     9541 ttaagtagcc atttttcaga ggcccagaga ataaatggaa tattgtacat aggtcgggtt
     9601 ggggtgtctt cgttacgtcc cagcatttta tgtggtggcg tatcgaatac ttcctctcca
     9661 tggtcagaaa aatagaaaag aaaaccatca ggggctgttg ctttgaaagt tttgatcaac
     9721 gttgcaacaa catggtcatt aaatatatta gcattatcgt aatcgttata taattccagt
     9781 tcattatcag taagtccttc tggtgcatgt tccctgtccc catcgaatac tccctgacct
     9841 tcaggataac ggtatttata tttgatgtgt gttcccaaca agtgaataat aacaaatttt
     9901 ttgggagctg gatctgccag tacttcacta aacggagcca gtacgttagt atcgtactca
     9961 cgggcacttt gcgttctctg ctgattcatg tagtactgtt tatctgtttg ccgagagaag
    10021 actgtcagca ttgtgttgcg tgcagtcatc gtttgttgat tggtgatcca gaatgttttg
    10081 taaccggcct gtttcattat attcatcagg gaaggttgtg tcaggtagag ttcgggctgc
    10141 tgttcatcag cgaaagtcaa tacctgctgt agtacttcga tggtatatgg tcttgaggag
    10201 acaacattat taaacacaaa catattttgt tccgttttgt ataattcatc tagtccgggg
    10261 gttgtttttc tgggatatcc atacagactc attctcccac gctgggttga ttctcctatc
    10321 accagaacca gtgttcgtgg ggtatctccg gaaatatcgt taaagttttt cagagcgggg
    10381 agcgcattat tttcctccag caaacggttc agagaattaa gctggagacg gtactgataa
    10441 tatccggtaa taaattgcca gggggcagcg ggctccatac gggatgccag gcgtcccaga
    10501 gtattgtcaa tagtcctgtt tttaacaaca gtatttatta atacagggtg cagaattaaa
    10561 ccgtagataa taaataaagg tataatgtag cgccagtatg atggtatata tatcgggcta
    10621 agacgagtcc atagcagaat agcaatagct gtatatattg tcgatataaa tagtattttt
    10681 aaactaaaat actgactaaa atattcgctg gcttcacttg tattactttc aaacatgacg
    10741 aacagtacac tctgagagaa ttcctgcccg tagattagat aatagcaaag ggcagcaaga
    10801 gatgtgcccc ataaaaccac gccgattaat gctgctgtaa cctttattct ctctgggaaa
    10861 agaaaaacag ggattaacca gagagagctg aaaagtagcg agtcacgaag tccattggtg
    10921 ccactatatc cagtcagaca aatgataatc tgaagtaaag tggaaaaata ccagaaataa
    10981 atgagagccc atcccagggc tttccagcta aaaacatgtt catg
//
LOCUS       DAZGJW010000145        11009 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZGJW010000145 DAZGJW010000000
VERSION     DAZGJW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.145"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..91
                     /locus_tag="ACHKIK_004012"
     CDS             <1..91
                     /locus_tag="ACHKIK_004012"
                     /inference="COORDINATES: protein motif:HMM:NF019442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO9085085.1"
                     /translation="HESAVKVDLNRPVTSSLLKRIRNGKKAKS"
     gene            69..1139
                     /locus_tag="ACHKIK_004013"
     CDS             69..1139
                     /locus_tag="ACHKIK_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9085086.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELLSGNRRESLIDRIKGADAITLHAWLDRYETILSERGIRPKTLLDYAS
                     KIRAIRRKLPDKPLADISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHV
                     ATNPVTATRTAKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCR
                     MKWSDINDNHLHIEQGKTGAKLAIPLTLTIDALNISLADTLQKCREASGSETIIASTH
                     HEPLSPKTVSKYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHK
                     SDSMAARYRDSRGREWDKIEIS"
     gene            complement(1154..1759)
                     /locus_tag="ACHKIK_004014"
     CDS             complement(1154..1759)
                     /locus_tag="ACHKIK_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HFO9085087.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKLYIKISLIINMLMCAGHY"
     gene            complement(1756..3444)
                     /gene="yfaA"
                     /locus_tag="ACHKIK_004015"
     CDS             complement(1756..3444)
                     /gene="yfaA"
                     /locus_tag="ACHKIK_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO9085088.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(3593..6220)
                     /gene="gyrA"
                     /locus_tag="ACHKIK_004016"
     CDS             complement(3593..6220)
                     /gene="gyrA"
                     /locus_tag="ACHKIK_004016"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO9085089.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6367..7089
                     /gene="ubiG"
                     /locus_tag="ACHKIK_004017"
     CDS             6367..7089
                     /gene="ubiG"
                     /locus_tag="ACHKIK_004017"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO9085090.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            complement(7218..10952)
                     /gene="yfaL"
                     /locus_tag="ACHKIK_004018"
     CDS             complement(7218..10952)
                     /gene="yfaL"
                     /locus_tag="ACHKIK_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO9085091.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGVVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVVDAGDGQVSLANN
                     NSYLGTTQIASGMLEVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         2665 a         2994 c         2670 g         2680 t
ORIGIN      
        1 ccacgaatca gcggtaaagg ttgacttaaa tcgaccagta acaagtagcc ttttgaagag
       61 gatcagaaat gggaagaagg cgaagtcatg agcgccggga tttaccccct aacctttata
      121 taagaaacaa tggatattac tgctacaggg acccaaggac gggtaaagag ttcggattag
      181 gccgagacag gaggatagca atcactgaag caatacaggc caatattgag ttgctatccg
      241 ggaacaggcg tgagtcactg atagacagaa ttaaaggcgc tgacgcaatc actcttcatg
      301 cgtggcttga ccgatatgaa acaatcctca gcgagagggg tatcaggccg aaaactctac
      361 tcgactacgc cagcaaaatc agggcaatcc gaagaaaatt gccggacaaa ccgctcgctg
      421 acatatcaac gaaagaggtg gcagcaatgc taaacaccta cgtagcagaa ggtaaagcgg
      481 cttccgcaaa attaatcagg tcaacccttg ttgacgtttt tcgtgaggca atagccgagg
      541 ggcatgtggc tacgaatccg gtaacagcaa cccgcacagc aaaatcagaa gtaaggcgtt
      601 caagactgac agctaatgaa tatgtcgcga tttaccatgc agccgaaccg ctcccaatct
      661 ggctgaggct ggcaatggat ttggctgtcg ttacagggca gagagtaggc gatttatgca
      721 gaatgaaatg gtcagacata aacgacaacc atcttcacat tgagcagggt aaaacagggg
      781 ctaagctcgc cattccgctg acgctaacga ttgacgcgct caatatctca ttggctgata
      841 cactacagaa atgcagggag gccagcggca gtgaaacaat aattgcatca acgcatcacg
      901 aaccgctttc cccgaaaaca gtatctaagt attttacaaa ggcgagaaat gcatctggac
      961 tctcatttga tggagacccg ccaacattcc atgaactgcg cagcctgtcg gcgaggctat
     1021 accggaatca gattggcgat aagtttgctc aacgtcttct cgggcataaa tcagattcaa
     1081 tggcggcgcg ttacagggac agtcgcgggc gggagtggga caaaattgaa atatcataat
     1141 ggttttattt tgattaataa tgaccagcgc acattaacat attgataatt aatgaaattt
     1201 taatatacaa cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag
     1261 ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc attaagtgct
     1321 gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcctggc
     1381 caataaactg gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccggttttcc
     1441 cgttcccctg attccagttt tgcgccagtt gacgctgtcc aggtgttagc gtcatctctg
     1501 gcggcaaata ctggctagag aaaccgttac tttttagcca tttactatcg tgaactttca
     1561 gcgtctcgtt cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac
     1621 ttggcccctg gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcccgg
     1681 attgttcgac attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg
     1741 ccagcagccc gtgcctcata gcggctgcca ggttatgggt agccactgcc aggcggcacc
     1801 gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt
     1861 cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact
     1921 ggtcaacgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac
     1981 gatgccatca gtgggtatta catccaccat tgccgggcgg tttttattca gtgtttgcag
     2041 ggcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag
     2101 cgacacgcgg aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg
     2161 atactgaccg tatcgggaac tcacttcgcg acgccagatt tgcgcttcgc cctgctgagt
     2221 ctggcttatc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt
     2281 aaacagtttc cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag
     2341 cggggtttgc aattttgagt ctgcatacca gcacagcccc gcggcaccgt ctaacgcccc
     2401 attcaacttg tcgttttcct gactgatgtg cgaaagcatc tcttcggcaa taccgtgtga
     2461 atacggcacc accacacaga agctggcccc ggcaggcata ctgttccaga ccggcgtaaa
     2521 atcgaaactg gtatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc
     2581 gtcgttaccc atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa
     2641 acccagcaac ctggcgctga ccacgatgcg ctggcgtact ggcgtttttt cagcagtacg
     2701 ctcttccagg ccaaagcttg cttgccagcg ttttttgccg ctcaacaaat cacctgcgat
     2761 cgccgtggct tcggtatcct gctgatcatc tttaaacaac atatccgtgc tggaaaacac
     2821 cagcatcttg tcctgataag tcgcgaacat cagggcgtta ttgccgttat agcgcaactg
     2881 ataaacagga acagtttcac tatttatctt gatgctactg atttccgttt tgcttaactg
     2941 gctgtcgctg gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg
     3001 gatcagcacc atataatgtg aaagatggcc ttgtttatcg tgccacagcg ctgcctgcgc
     3061 gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac
     3121 aatacgacga atgctgcctt caatgcccag acgatcggca tgattctgat aatagaaaac
     3181 gaaatcttcg ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg
     3241 gctcagagag tcgctgtcga gaaagaggtc cggctcattg agatcgattt gcagattgtt
     3301 gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca
     3361 gacgccagcg gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc
     3421 tttcgccttt ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac
     3481 gggcacagcc aaactttacc gtgccctaat acgacaaaag cccagacttt gcagcctgga
     3541 cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt
     3601 cttctggctc gtcgtcaacg tccacttccg gagcgatttc atcgtcccct tccgcggcac
     3661 tgccatcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgt tgcagaccca
     3721 ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcctacga
     3781 tgctgatttc cgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct
     3841 ggtcgcagtc atctacctgt accgcgccaa ccactaaacc attacgttca gtaaccttaa
     3901 tggagataac ccctttcgtc gcacgcgact tggttgggta ttccgccact gcggtacgtt
     3961 taccgtaacc gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag
     4021 agacgacttt atcgccttca cctaagcgaa taccgcgaac accggtggtg ttgcagccca
     4081 tcgcacggac agaagactct ttaaagcgca ccactttacc ttcagcggag aacagcatta
     4141 cttcgtcttc gccgctggtc aggtcaacgc cgatcagctc atcgccgtca accagtttga
     4201 tcgccacttt accggcggta cgcagacggt tgaactcggt gaggacagtt ttcttcacgg
     4261 taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc actggcagga
     4321 tcgcagtgat acgttcgtcc tgctccagcg gcagcaggtt gacgatcgga cgaccgcgcg
     4381 cgccacgagt ggcttccggc aactgataaa ctttcatcga atagacgcga ccacggctgg
     4441 agaagcacag aatatggtcg tgagtgttcg ccaccagcag tcggtcgata aagtcttctt
     4501 ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa
     4561 gcggctgata cttaacgtag ccctggtgag agagcgtcac taccacatct tcctgagtga
     4621 tcagatcttc caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac
     4681 cgaactgttc acgaaccagc tccagctctt cacggatcac ttccatcaaa cgatcggcgc
     4741 taccaagaat acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca
     4801 gtttttcgtg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt
     4861 gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc agccattccg
     4921 gacgcgcagc atcgtcgcca gcacgttcga gcatcgcggc aacgttgccc agctgccacg
     4981 gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga
     5041 tgatcgggtc gatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc
     5101 gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa
     5161 acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca
     5221 tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt
     5281 caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt
     5341 cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga
     5401 tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgaccgg
     5461 ttttggcgtc aacttccact tctgcgcgag cgcggatata caccttgccg cgaccggtac
     5521 ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc
     5581 ccgggatgtg ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac
     5641 aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga
     5701 taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt
     5761 tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca
     5821 gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc
     5881 cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct
     5941 gcgccatacg gacgatcgtg tcataaaccg ccgagtcacc atggggatgg tatttaccga
     6001 ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta
     6061 cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca
     6121 gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttc agctcttcct
     6181 caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact
     6241 gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat
     6301 acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa
     6361 gtgccaatga atgccgaaaa atcgccggaa aaccataacg tagaccacga agagatcgct
     6421 aaatttgaag ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac
     6481 cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa
     6541 aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc
     6601 gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa actgcacgcg
     6661 ctggaaagcg gcattcaggt ggattatgtg caggaaaccg tggaagagca cgcggcaaaa
     6721 catgccgggc agtatgatgt ggtgacctgc atggagatgc tggagcacgt ccccgatccg
     6781 cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg
     6841 acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg
     6901 cgcatggtgc ccaaaggcac gcatgatgtg aagaagttta ttaaaccggc agaactgctt
     6961 ggctgggtag atcagacaag ccttaaagag cgacatataa ccgggctaca ttacaacccg
     7021 ctcactaata cttttaaact cggccccggc gtggatgtga actatatgct gcacacgcag
     7081 aataagtgag gttgttgttt ggccgagcca atgcctgatg cgacgcttgc cgcgtcttat
     7141 cagtatctac aaatgctccc cgtaggccgg ataaggcgtt tacgccgcat ccggcaaccg
     7201 tgccgactag acagtgatta ccatttcacc gtcatcgaca aaaaccctgc cgtctgggca
     7261 aaatcatcac tccctttctg ccacgccacg ctgccgcgca gggacactcg ctgactgata
     7321 ttgcccgtga ctcccacttt tatttcaccc cgtcgcttca ccgcatcgtc actgatagtg
     7381 ctgccatctt cttcaatttc cgtcgcatgg ggatcgtgat aataattcag atctaatgtt
     7441 ggtgtgacac caaccgcggt acgccattcg ctgtgtaaac ccagccgcgt ctgaatatca
     7501 tccccctgcg attgtgacac gcgcgcatgg ttagcggcgg taaaatcatc ctgctgcacg
     7561 ccctgataaa tcacctgcgc ctgcggttca atcaccacac cacgccccgg taaccactga
     7621 tacccggctt ccagcgaggc gataatcccc gacgagtggt aatgatctgt gccatcgtct
     7681 tgttcggaaa catcattgtt aaaccacgcg tattgcagcc agctatccag ccaggccccc
     7741 tgcttctgat taccgtgctg ataccagctt gaggtcagcc caacggcata accgtggttc
     7801 tggttatcgg cgcgagttcc ggtcatattc gagcggctct cgccctgatt atcactgtag
     7861 ccaccaaccg ccccaagcat ccactcgcca tcatcgcccc agcgcccgct aaacaggtca
     7921 ccgctaagct gcaccgtaga agtgtcttca tgctgagcca gttgccctgc cgccgtgtaa
     7981 tgataacgtc cgccgataac acgtaaattc agcgtctgac cattgccacc tgcatgatcg
     8041 cgtcgctcca tcataaacgc ctgatttgcc gcccgcagat tattgagata accgccaact
     8101 ttggcattca gcaccggctg gtaagcgggg attggatcgg gtgttgggtc aggatcgggt
     8161 gttgggtcag ggtctggatc aggtggcaat ggtggcgtta cttcttgcga tcgcagatac
     8221 cagtcgttgt tatcttccac cagcgtgtag tcatatgctc ccatattgac gtagccgctg
     8281 cctgccagac tgaacttcgc attgttttga aactgcgtgg gatcggctgc gaaatcaacc
     8341 actttaatgc ctgtcgatgt cggctcacca atccctgtaa tggagttaac caccacagtt
     8401 gtgttgccag cagtattacc gttcaacacc aattgatcgc ttaccgagtc atcgccgttt
     8461 aattcgctat cgagcagtag cgtaccgccg ccggtataat caccgtttac cgtcagcgta
     8521 tcgccagcga cgccattttg caggctaacc gcaccggacg tattggtcag attgccatta
     8581 accgtcacgt cggaaagata ggtcaggctg tcagtttcaa ggttggtatt aagctcacct
     8641 gtcaacgtca cgccatcggt ggcattcacc agcgtcccat cgccgttaaa cagtgaggca
     8701 ttaagggcga cagaagtatc ctgcccggtc aggcgcaaaa ccgtaccatc gctgatgtcg
     8761 atagtgccgc tggaagtagc atcaatagat tgaatatcct gatccgcgcc agtaacgaac
     8821 gtcgccccat caccgaccca cagtgacgaa gcataaggaa ggatatccgc gacatcgcct
     8881 ttcagggtgc cttcttcgac acgtaccgcc gactgcgttg taccgctggc ggtcagctcc
     8941 agcgtacccg cccccgtttt agtcaatgtg ctaccctcat cctgatgctg cccgctgctg
     9001 tcagccatca gtgcgcccca ctgcgtgtct acccccgcat caactgccac ttcaccgtcg
     9061 gcgcgcattt caatatcacg cccatgctca gtcgtcgtag agcgagtatc gacattggca
     9121 gtaatctcca tcacgctttc ttgttgctta tcggtaaaga taacctggcg gttatagtgg
     9181 gtatctccaa gctgtgagtt gtcgctcacc tccagcatac cggaggcgat ttgtgttgtg
     9241 cccaaataac tattattgtt tgccagactt acctgcccgt cgcccgcatc gaccaccacg
     9301 tagacgccat ctttcccgtc accgccgata tgcatatcgt ttccacctgc aagatctacc
     9361 acatcctgac tgccgataac cgttccgctg ctgccgctga cttcaaggcc atcgttgtat
     9421 gatgtgccac tctgccaggt ggagaaatca gagagatcga gcacaccgcc gtttaacacg
     9481 atcgactgcg gatcgtcctg gagagcggta agatctgccg cgtcgccttc cagcgaaagc
     9541 accgcaccat catcaacgac tatatcgccg gttagcgcca tcgactgcgc ccccgacagc
     9601 acgtagctgc cgttttgcgc aatggtgagc tgaccagcac cttcgatggt gccagcaaaa
     9661 ctgccttgat taacagtaac attgccacca gcatcgatat ttaaagtgcc attctgaaag
     9721 cccgtcaatg agtgcacaaa ggtttgttgg gtggagccaa cgtttagttc tgcctgattc
     9781 tggtacttat caatactccc tatcgtcaga ccgtagcagt cttgcggatc gtcctggcaa
     9841 tgcgtatcgc cgacattcat cagggagttg ctgcgaccca gggtaacttc accgttttca
     9901 atctgcatct caccagtaaa gtcattgtta tctgcattaa gtaccagatc gccggaacct
     9961 gttttggtga tcaacccggt accagcaata gagtcaacag ctccgtcatt ctctgtattg
    10021 ccaataacca gcgtttttcc gtcggcaata tcaaaggtaa cttcacttaa gccgagatac
    10081 ataaagccac ccgccgcagt ggaaggacca tctccatagc ctgctgcgct gttgttctca
    10141 tccactaaca cgccgccgtt ctggctgtag ctgtcatcaa cagaaatatc aataagatag
    10201 ggagccgtcg cgctattggt atatatcgcc ccgccataac cttcggcagt gttatttgta
    10261 aaggcggtgt tatttattat cgtataacct gaaggatgct tgctgtcgct attattatcg
    10321 gtaacatcga ttgccccgcc atcgccatca ctgtaacttg ttgatgtata tgcctggttg
    10381 ttatcaaaaa taacatcact taaataaacg tcattattaa tggtataaat tgcgccacct
    10441 ttgccatcat tagcgatgtt attgcgaaac atggcgttag tgacacgtaa atcgacgaca
    10501 ccagtatcat tagtaccaga agaatagatt gcgccaccat agccgcctgc gacgttacca
    10561 gaaaaaataa catcagtaag attcagcgtt gagttttctt tagcaaatat ggccccgccg
    10621 ttattatatt ctcctgtaac ggtgttattg gcaaacagag tcattccagt ttcattttct
    10681 ggtaatagtg aaaattccgc tccttgttgc aaaaatacgg ctcctccgct ggcgttattg
    10741 gtcatatccg aaaaaaccag ccattgccca tcggcgacac tccagtcctg cgtaatgcct
    10801 gaaaggcttt gcctgctggc gtgacaactc gctttgatat catatccctg gcataaatca
    10861 atggccgccg cgaaaccgtt agcagagaaa agagatgcaa tcattgacgg gagtagagat
    10921 aaatactcct tgcgtagaaa gataatccgc atattcatct aaccatcatt ttatataaga
    10981 cggcatattt aaacgcatta tacaatatg
//
LOCUS       DAZGJW010000146        11000 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZGJW010000146 DAZGJW010000000
VERSION     DAZGJW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.146"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            160..774
                     /gene="evgA"
                     /locus_tag="ACHKIK_004019"
     CDS             160..774
                     /gene="evgA"
                     /locus_tag="ACHKIK_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO9085092.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            779..4372
                     /gene="evgS"
                     /locus_tag="ACHKIK_004020"
     CDS             779..4372
                     /gene="evgS"
                     /locus_tag="ACHKIK_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO9085093.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4428..5573)
                     /gene="yfdE"
                     /locus_tag="ACHKIK_004021"
     CDS             complement(4428..5573)
                     /gene="yfdE"
                     /locus_tag="ACHKIK_004021"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO9085094.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPCLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5647..6591)
                     /gene="yfdV"
                     /locus_tag="ACHKIK_004022"
     CDS             complement(5647..6591)
                     /gene="yfdV"
                     /locus_tag="ACHKIK_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO9085095.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6661..8355)
                     /gene="oxc"
                     /locus_tag="ACHKIK_004023"
     CDS             complement(6661..8355)
                     /gene="oxc"
                     /locus_tag="ACHKIK_004023"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO9085096.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8409..9659)
                     /gene="frc"
                     /locus_tag="ACHKIK_004024"
     CDS             complement(8409..9659)
                     /gene="frc"
                     /locus_tag="ACHKIK_004024"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO9085097.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10172..10804)
                     /locus_tag="ACHKIK_004025"
     CDS             complement(10172..10804)
                     /locus_tag="ACHKIK_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO9085098.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNASVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         3223 a         2473 c         2265 g         3039 t
ORIGIN      
        1 ttgacatttc atttatgccg actatttata tggtatactt gtcgaattat cttaaaggaa
       61 gctcagattt tcttattttt attgagaaaa tgagatgacg ccttatgtct gtattactac
      121 agggagaagg gagatgcttc attgcaaagg gaataatcta tgaacgcaat aattattgat
      181 gaccatcctc ttgctatcgc agcaattcgt aatttattga tcaaaaacga tattgaaatc
      241 ttagcggagt tgactgaagg gggaagcgcc gttcagcggg tggaaacact taagcctgat
      301 atcgtcatca ttgatgtcga tatccccgga gttaacggta tccaggtgtt agaaacgctg
      361 aggaagcgcc aatatagcgg aattattatt attgtctccg ctaaaaatga ccatttttac
      421 gggaaacatt gtgctgatgc tggcgctaat ggattcgtga gtaaaaaaga aggcatgaac
      481 aatatcattg cggctattga agctgcaaaa aatggctact gctatttccc cttctctctc
      541 aaccggtttg ttggaagttt aacgtccgac cagcaaaaac tcgactcctt atcgaaacaa
      601 gaaattagtg tcatgcggta tattcttgat ggcaaggata ataatgacat tgctgaaaaa
      661 atgttcatca gcaacaaaac tgtcagcact tataaaagtc gcctgatgga aaaattagaa
      721 tgtaaatcac tgatggatct ttacacattc gcgcaacgta acaaaatcgg ctaatcacat
      781 gaagttttta ccctattttt ttcttctctg ttgtggtctt tggtcgacca taagtttcgc
      841 agacgaagat tacatcgaat atcgtggcat cagtagtaac aaccgtgtca cacttgatcc
      901 agtacgtctg agcaacaagg aattacgttg gttagcgagc aaaaaaaatc ttgtgattgc
      961 agtacacaag tcccaaacgg ctaccttgtt gcataccgat tcgcagcaac aggttcgtgg
     1021 tattaatgct gattatttaa atcttttaaa aagagcgtta aatatcaaat taacactccg
     1081 ggaatacgcc gatcatcaaa aagcaatgga cgcgcttgca gaaggtgaag tcgatatagt
     1141 gttatcacat ttagttactt cgccgcctct taatagtgac attgctgcaa ccaaaccatt
     1201 gataattacc tttccggcgc tggtaaccac ccttcacgac tcaatgcgac cgcttacctc
     1261 accaaaacca gtaaatattg ctcgggtagc aaattacccc ccagacgagg taattcatca
     1321 atcgtttcca aaagcaacaa ttatctcttt tacaaattta tatcaggcat tagcatccgt
     1381 atcagccgga cagaatgatt actttattgg tagtaacatc attaccagca gtatgatttc
     1441 ccgctatttc actcactcct taaatgtagt gaaatattat aactcgccgc gtcaatataa
     1501 ttttttcttg accagaaaag aatccgtcat tcttaatgaa gtactcaata gatttgttga
     1561 tgctttaaca aatgaagttc gctatgaagt ttcacaaaat tggcttgata caggaaacct
     1621 ggcctttctg aacaaaccat tagaactcac tgaacatgaa aaacagtgga ttaagcaaca
     1681 tcccgattta aaggtgctgg aaaatcctta ctctcctccc tattctatga cggatgaaaa
     1741 tggctcggtt cggggcgtta tgggggatat tcttaatatt attaccttgc aaacaggttt
     1801 aaatttttct ccgatcaccg tttcacacaa tatccatgcc ggaacacagc ttaaccccgg
     1861 tggatgggat ataatacctg gcgctattta tagtgaagat cgagaaaata atgtttcatt
     1921 tgcagaagct ttcataacaa cgccatacgt ttttgtcatg caaaaagcac ctgacagtga
     1981 acaaacatta aaaaaaggaa tgaaagttgc cattccatat tattatgagc tgcattcgca
     2041 attaaaagag atgtatccgg aggttgaatg gatacaggtc gataatgcca gcgctgcatt
     2101 tcacaaggtt aaggaaggtg aacttgatgc tctggtcgcg acacagttaa attcgcgtta
     2161 catgatcgat cattactatc ctaatgaact ttatcatttt cttattcctg gcgttccgaa
     2221 tgcatcgctt tcgttcgctt ttcctcgcgg agaaccggaa cttaaggata ttattaataa
     2281 agcactgaat gcaattcccc caagcgaagt tctgcgcctg acggaaaaat ggattaaaat
     2341 gcccaatgtg accattgaca catgggacct atatagcgag caattttata ttgttacgac
     2401 attatccgtt ttattagttg gcagtagcct tttatgggga ttctacctgt tacgctcagt
     2461 tcgtcgtcgt aaagtcattc agggtgattt agaaaaccaa atatcattcc gaaaagcact
     2521 ctcggattcc ttaccgaatc caacttatgt tgtaaactgg caaggtaatg tcattagtca
     2581 taatagtgct tttgaacatt atttcactgc ggattactac aaaaatgcaa tgttaccatt
     2641 agaaaacagt gactcaccct ttaaagatgt tttttctaat gcgcatgaag tcacagcaga
     2701 aacgaaagaa aatcgaacaa tatacacaca ggtatttgaa attgataatg gcatcgagaa
     2761 aagatgcatt aatcactggc atacattatg caatcttcct gcaagtgaca atgcagtata
     2821 tatttgtggt tggcaagata ttactgaaac gcgtgatcta attaatgcac tcgaggtaga
     2881 aaaaaataaa gcgataaagg ctaccgtagc aaaaagtcag tttctggcaa cgatgagtca
     2941 cgaaataaga acaccaataa gctctattat gggcttcctg gaacttctgt cgggttctgg
     3001 tcttagcaag gagcaacggg tggaggcgat ttcacttgcc tacgccaccg gacaatcact
     3061 cctcggctta attggtgaaa tccttgatgt cgacaaaatt gaatcgggta actatcaact
     3121 tcaaccacaa tgggtcgata tccctacttt agtccagaac acttgtcact ctttcggtgc
     3181 gattgctgca agcaaatcga tcgcattaag ttgcagcagt acatttcctg atcattacct
     3241 ggttaagatc gaccctcagg cgtttaagca ggtcttatca aatttactga gcaatgctct
     3301 caaatttacc accgaggggg cagtaaaaat tacgacctcc ctggttcaca ttgatgacaa
     3361 ccacgctgtt atcaaaatga cgattatgga ttctggaagt ggattatcgc aggaagaaca
     3421 acaacaactc tttaaacgct acagccaaac aagtgcaggt cgccagcaaa caggatctgg
     3481 cttaggctta atgatctgca aagaattaat taaaaatatg cagggcgatt tgtcattaga
     3541 aagtcatcca ggcataggaa caacatttac gatcacaatc ccggtagaaa ttagccagca
     3601 agtggcgact gtcgaggcaa aagcagaaca ccccatcaca ctacctgaaa agttgagcat
     3661 attaatcgcg gatgatcatc cgaccaacag gctattactc aaacgccagc taaatctatt
     3721 aggatatgat gttgatgaag ccactgatgg tgtgcaagcg ctacacaaag tcagtatgca
     3781 acattatgat ctgcttatta ctgacgttaa tatgccgaat atggatggtt ttgagttgac
     3841 tcgcaaactc cgtgagcaaa attcttcctt acccatctgg gggcttacag ccaacgcaca
     3901 ggctaacgaa cgtgaaaaag ggttaagttg cggcatgaac ttatgtttgt tcaaaccgtt
     3961 gaccctggat gtactgaaaa cacatttaag tcagttacac caggttgcgc atattgcacc
     4021 tcagtatcgc caccttgata tcgaagccct gaaaaataat acggcgaacg atctacaact
     4081 gatgcaggag attctcatga ctttccagca tgaaacgcat aaagatctac ccgctgcgtt
     4141 tcaagcacta gaagctggcg ataacagaac tttccatcag tgtattcatc gcatccacgg
     4201 tgcggctaac atcctgaatt tgcaaaagtt gattaatatt agccatcagt tagaaataac
     4261 acctgtttca gatgacagta agcctgaaat tcttcagttg ctgaactctg taaaagaaca
     4321 cattgcagag ctggaccagg agattgctgt tttctgtcag aaaaatgact aaatagcggc
     4381 tcccacaatg ttcaaatgtg ggagctattt accagcacat cttacattta tgatgagaac
     4441 tcctggcgaa tttgttcccc atgctggtcg agcgttgccg ctcccggcat aacatgcggg
     4501 tccgcgcagc cgctgatttt tatcggatta cccggcatca ttattccccc ggcttcaatc
     4561 aacatatttc tcgcctgagt ttgtggcaat ttaatggcct cagccacact taataacggc
     4621 gcgacgggta caccaacttc atgtattctg gctaaccaaa cttcagctgc ctgcgttttt
     4681 aacgtccgct caatatattg tttaagaata gcctggtttt gtacgcgtaa aatattgctg
     4741 ctaaatcggg gatcattaac cagttccgta agctccagtg cctggcataa cgcagaaaaa
     4801 agcttgtcat taccacaaca aatcgtaatc ggcttatcct gagtattgaa aacatcaaaa
     4861 ggtgccatgt agggatggcg atttcccaga cgttgtggtg acttccctgt cgcgatatat
     4921 gccatcagac catgctccag aaaactcagc gtggcatcaa acatcgctat atcgacatgc
     4981 gcccctctct ggctcttttc gcggccataa agtgcactca ctattccgct gaataaatag
     5041 acaccgccgc atagatccgc aagagatgta ccaacgcgca ctggcggagc atcaggatat
     5101 cctgtttcca tcattatccc gctcattgcc tgaatgatgg tatcgtaggc aggagcatct
     5161 tttagcggac cggtatgtcc gaaacctgac gatgaagcat atatgaggca cgggttgatt
     5221 tcttgaagcg tttcccatga aaaccccagt ttttccattg tacctgggcg aaaattctca
     5281 gctaatacat cagcttgttt gagcatattt ataaatatac ttttatcgtg atcattcttt
     5341 aaatcaagaa ccacactctc tttgccatga ttaataaaac tgtaatagag tgactgtcca
     5401 tccacatagg gaccaaatgt gcgggtatca tcaccatgac ccggcggctc aactttaatt
     5461 acccttgcgc ccatattaca aagaagttga gttccgaaag gtccattaag gacatgtgtc
     5521 atatcgataa ctaataagcc ttcaaacggc cctttgcttt cattatttgt catttgctta
     5581 tgccttgaaa aaagccgaag gtgaaaagac acttcggcat ttatgtaaaa tcaataacat
     5641 gattaattat gaaaccagtc gactgacata aatccacaag ggagccgtga cgacaaagcc
     5701 cagtacgctc acagccaatg acgctgtacc agtacgggtg tagacattaa accggctggc
     5761 aataatgatt ccggagaatg ccggcggtaa tgcgcctgcc agtaccatca tctgcagatg
     5821 ttcgctgttc aaatgacatg ccataccaac gagaagcagt gccagtggca tcagaatcag
     5881 cttcaggaag gtgttataag caatttcggc actgaactcg aatttatgtg cagccagagt
     5941 caaccccgca gcgaataccg ctacccctga gttagccttc gcaatcagat taaaggttgg
     6001 gtcccatgct gccggaattt ttaccccaac caacaccagg atcgttgcca gaacaggtgc
     6061 ccatactact ggctcctttg ccgcagaaat taatgcgctc agattactat tcttcttacc
     6121 atccgctcct gaggaaggat tcagcaaata cagaccaata ggaatagtaa ttgcgttaac
     6181 aataatagaa ataattgcta ccactaaacc tgttgatacg gaatcaccat aaataggatc
     6241 gagaactgca aaccccagga atccaatggt aggtgaaccc gcaattaatg cacatacagc
     6301 tgcttctgca tgggtacgtt taaaaaattt gtagcaacca aaccaggaga aaaagaaaca
     6361 tccgacaata accacaagtg atacaagggt cagacgagtg tccgcaaaaa tcatttccct
     6421 gtttgcccgg gtaatagaaa caaatagagc cgcaggaaga gcgtagttta ataccagttt
     6481 attaaatgcg cgggcttgat cttctgaaaa agtttctcgt ctgccgctaa aataacccaa
     6541 cagcatgatt acgataatag gcaataaatc gccaataaaa aatgttagca taatagtatt
     6601 tccgataagg gtcccctcac catcgcataa tgagttggca agaggagacg atgtcagtat
     6661 ttaattacca gcgacttgtt ttgggttaag tttggtaata tggccacttt cagttcctgc
     6721 tgcagggtcg atgaccacat taataatggt cggtttgcgc gactgaatac cggtggttaa
     6781 agcatgacga agttcatctg tcgtggtgac gttatagcca acgccacgaa acgcatccat
     6841 taatttgtca taccttgcat ggtgcaacag atccgttggt gatggtgcac cagcgccact
     6901 gagatcaaca ccgtctcctc tgtagatgcc gccattatta aaaataacga tcgtcaccgg
     6961 caggttatat cgacaaatcg tttcaatttc catcccactg aaaccaaaag cactatcacc
     7021 ttcaatggcg acaaccggag aaccagaagt cacgctagca ccgatggcat agcccatacc
     7081 gatgcccatg acaccccagg taccacaatc cagacgacga cgtggtttat acatatcaat
     7141 aatatttcgt gcattatcca gggtatttgc accttcatta actaaataaa tatcctggtt
     7201 ctcgcgcaat acgtcgcgca cagcacttaa tgcattaaag taatttaatg gttgagtatc
     7261 tgtacttaat ttttcatgca ttttttgtgc attttgctgc ttgtggatat ttaaaatatc
     7321 gcgccatacc agtggagtcg taaatgtgtt ttgtttcagt tctgccagca taccttgcat
     7381 actggatgca atatcaccaa cgactggcac agcaatgggg cggttgctgt caatttcctg
     7441 cggttcaata tccagttgaa taaactgtgt atctgccgcc catccttttt taccgtgtgc
     7501 caataaccaa ttcagtcgtg caccaacaag catgacaacg tcagcatttg ccagggcaaa
     7561 cgaacgcgca gctgccgcag aaagtggatg cgtatcttca aggatccctt tcgccataga
     7621 cattggcagg aatggaatct gagtactttc aataaactca cgaagctgtt catcagcttg
     7681 tgaatacgcc gcgcctttgc caaggataat taatggccgt tcagcttttg ctaaaagcga
     7741 aattgcgcta gtgactgact tcgggcatgg caataatgct ggcgacggat tttcaacttt
     7801 aacaatcgtg gttaacgctt cgtctttttc catcgtcgcg gccaggacat ttgctggcaa
     7861 atcaagataa actccgccag ggcgacccga tacagagacc cggatagcgc gtgccaatgc
     7921 aatgccaaga tcctgcggct gattaacgcg aaatgctgct ttggcatacg gttttgccgc
     7981 attcatttgg tccagctctt cataatcacc ttgctgtagg tcgacgatcg cgcggtcgct
     8041 ggagccgcta atcatgatca tcggaaaacc atttaccgtt gcgttggcca atgcggtcaa
     8101 accattgagg aatcctggcg cagaaactgt caggcagatc cccggttttt gggtaagaaa
     8161 accgcttgcc gcagcggcat agcctgccga ctgctcatga cgaaaaccaa tataacgaat
     8221 gccttccgcc tgggcatggc gtgccatatc cgtcacagga atacctacaa caccataaat
     8281 agtgtcaata ttattctgtt ttaatgcttc aacgatgata tgcataccat ctgtcatttg
     8341 aagttgatct gacatgatta ataacctcga aatttttata aattgggagc cagaagcccc
     8401 cgttaatatc agatggcgtg gttttgcttc attgcagcaa tttcatcgtc gctataaccc
     8461 agctcctgca atacagcagc ggtatgttca cctaatagcg gcgcagcttt aatatccggc
     8521 gtaaaggcag agaatttcat tggacaacca acggtcagat attttccacg caacggttgt
     8581 tccacttcaa caacactgcc actttggcgc aaagagggat caagtgaaat ttctttcata
     8641 cttaaaaccg gtgcacaagg aatatcaaac tgagtcaaat aggccaccgc ttcatgttta
     8701 tcaatagtga cagtgtattt ttcgatttca gcaaaaatat cgaaaatatg tggctgtcgt
     8761 gcatgggctg tactgtatgc cggatcggta atccattctg gtttgccgat ggctttacag
     8821 gtgttttccc agttttgctc ctgaatagtg aaataaatat aggcgttagg atcggtttcc
     8881 cagcctttac atttcaggat ccagccaggc tgaccgccac cacctgcatt accaccgcgg
     8941 ggaactgcat caccaaatgt accattcgga tactgcgggt attcttccag ataacccaat
     9001 ttatcgagac gctgctggtc acgtaatttc acgcggcaaa ggttcaatac ggcatcctgc
     9061 attgacatgg tgactcgttg cccacgcccc gttttttcgc gatgcagcaa agcagcaagt
     9121 aaaccgatca gcaaatgcat tccggtgttg ctgtcaccca acgctgcagc gcttaccagc
     9181 ggcggaccat cccaaaaacc cgtagtggat gccgcgccac ccgctgcctg agcaacgttt
     9241 tcataggctt ttacattcac ataaggcgaa cactcatcaa accctttgat cgaaccaaaa
     9301 atcagacgtg gattgatttc ttgaatatgc tcccaggtga agcccatgtg atcaatggcc
     9361 cctggatgaa agttctcgac taagatatca gcttcgcgga tcagcttttc cattacctct
     9421 ttgccttccg ctgttttggt atttaactca atagaacgtt tgttactgtt aagcatggtg
     9481 aagtaaagcg catcgatatc aggaatatct cgcagctggt gacgcgttac gtcaccaacg
     9541 ccgggacgtt caattttaat aacgtcagcg ccaaaccagg ccagcatttg agtacaagat
     9601 gggccagatt gcacaccggt gaaatcgaga actttaattc cttgaagtgg agttgacata
     9661 gcaataccta ttagttaatt aaatatgtgt taatttagcg tttatttaag gcgtaatatc
     9721 ttctataaat aacccgcaaa atatcgtcgt agctgcactc gatattttta aaatcctgta
     9781 tcaagtgctg aaaatcattt catcgccact tccaattcct ttctgcatgg taagtagtga
     9841 ggacgaaaag aagaatatga taaatattga ttcgtggtaa tcagagagag tcgcgacatt
     9901 tgtgatgtta ttcttacaac tgtaagaagc gccggtgatt tacaagtaag ggtatgatta
     9961 cgctttttaa agcgtaccat aaaacataac ataataaatg atttgtgatg acacgggatt
    10021 attgggattt actttgagga agataaaatg cgtgtttata gaaccgcgag cgccaaataa
    10081 ttttatgacc acaacctgtc gtattgtggt cataaaaaat tgtcggctgg agggagttaa
    10141 atcctttacc tcaatatact gcctgtcagc attagttcac aaacagtgct tcgctgtcga
    10201 cgatgattcc atcttccgca cctttaagcg ccaggtttgc ctcgtagtat tgctttttat
    10261 caagcagttt ctgcgcatca gcaagtgcat tacgagtctg tttaagcggc atcagatatt
    10321 ggttttccat tacgccaaca ccggccaggc gaagttcttc cattgcgccc tttttatccc
    10381 ctttcgccat tttttcattg gcaattttta tcgcggcttc tttttcggga gttgctacat
    10441 aactctcaga aattccgaca gatgcattaa tgacaatata ttggtcatca ttgagattgg
    10501 tctttttacc tggcttagcg aactttgccc attcggtact gtcatctgac agcaaagcgg
    10561 aagcttcatt ggtcagttct tttgcctttt ctggatcgcc atgaaacaga gccaggcgcg
    10621 ctacctgcac gtcacgcatc gcatataaac cttgttcaga gatacgctct gctgctgccg
    10681 ttttctgggt ttgctgaact tgctgagttt gctgttgtgc cgctaccgat gcattatcag
    10741 cagcccatac agttgaagat gccagaattg ctgtgaccat cgtagccata attaaacgtt
    10801 tcatacttac ctcattcttt aacagtttta gaaaacgccg ccatcatatt gggtgagcat
    10861 gaaatgagaa atcagagagc aacggtcatt ttgaaagggt tatcttacag ttgtagttat
    10921 tatcgcaaga agtattttag tcttacaatt gtaggaatct gcttcctgtc gcagagaact
    10981 attcttatgt accacaaaga
//
LOCUS       DAZGJW010000147        10991 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZGJW010000147 DAZGJW010000000
VERSION     DAZGJW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.147"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..607
                     /locus_tag="ACHKIK_004026"
     CDS             <1..607
                     /locus_tag="ACHKIK_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO9085099.1"
                     /translation="KDIAEKIQKQGGDYLFAVKGNQGRLNKAFEGKFPLKELNNPEHD
                     SYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEM
                     TVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIR
                     HIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            876..3113
                     /gene="nrfB"
                     /locus_tag="ACHKIK_004027"
     CDS             876..3113
                     /gene="nrfB"
                     /locus_tag="ACHKIK_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO9085100.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMFNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            3100..6072
                     /gene="nfrA"
                     /locus_tag="ACHKIK_004028"
     CDS             3100..6072
                     /gene="nfrA"
                     /locus_tag="ACHKIK_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO9085101.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAAQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            6073..6963
                     /locus_tag="ACHKIK_004029"
     CDS             6073..6963
                     /locus_tag="ACHKIK_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO9085102.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7146..7907
                     /gene="envY"
                     /locus_tag="ACHKIK_004030"
     CDS             7146..7907
                     /gene="envY"
                     /locus_tag="ACHKIK_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO9085103.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            8420..9373
                     /gene="ompT"
                     /locus_tag="ACHKIK_004031"
     CDS             8420..9373
                     /gene="ompT"
                     /locus_tag="ACHKIK_004031"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO9085104.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            9560..>10991
                     /locus_tag="ACHKIK_004032"
     CDS             9560..>10991
                     /locus_tag="ACHKIK_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9085105.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKRENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFIAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITP"
BASE COUNT         2977 a         2711 c         2665 g         2638 t
ORIGIN      
        1 gaaagatatt gcagagaaga tacaaaaaca gggaggtgat tatttattcg cggtaaaagg
       61 aaaccagggg cggctaaata aagcctttga gggaaaattt ccgctgaaag aattaaataa
      121 tccagagcat gacagttacg caatcagtga aaagagtcac ggcagagaag aaatccgtct
      181 tcatattgtt tgcgatgtcc ctgatgaact tattgatttc acgtttgaat ggaaaggtct
      241 gaagaaatta tgcgtggcag tctcctttcg gtcaataata gcagaacaaa agaaagagcc
      301 cgaaatgacg gtcagatatt atatcagttc tgctgattta accgcagaga agttcgccac
      361 agcaatccga aaccactggc acgtggagaa taagctgcac tggcgtctgg acgtggtaat
      421 gaatgaagac gactgcaaaa taagaagagg aaacgccgca gaattatttt cagggatacg
      481 gcacatcgct attaatattt taacgaatga taaggtattc aaggcagggt taagacgtaa
      541 gatgcgaaaa gcagccatgg acagaaacta tctggcgtca gtccttgcgg ggagcgggct
      601 ttcgtaatct tgccctgcct gctcacctcc tccaggcact tccactacat tatgacctgc
      661 tcggtgccac cgctcctttt cactatgacg ttaacctccc ctccttgaca ccatgctttt
      721 gtctacattc tacgcaaacg gcaactttta taaccagtgt aaaaataacg tgccgcaata
      781 gtcataccta ttaatggtaa aaagctgtca caattcataa aaaaccttaa tatacgccac
      841 cctaaacata accagcgtta atgtaaggtt tttgtgtgga ctggcttctt gatgtttttg
      901 caacctggct ctacggctta aaagtaatcg cgataacgtt agcggtcatc atgttcatca
      961 gcgggctgga cgattttttt attgatgtcg tctactgggt acgccgcatt aaacgcaagt
     1021 tgagtgttta tcgccgctac ccgcgaatga gttaccgcga actgtataaa ccagatgaaa
     1081 aaccgttagc gattatggtt ccggcgtgga atgaaacggg cgtcatcggc aatatggccg
     1141 agctggcggc gaccacgctc gactacgaaa actatcatat ctttgttggc acctacccca
     1201 acgaccccga tactcaacgt gatgttgacg aagtgtgcgc tcgctttccg aacgtgcata
     1261 aggtagtctg cgcgcgtcct ggtcccacca gcaaagccga ctgtctgaac aacgtgctgg
     1321 acgccatcac ccagtttgaa cgtagcgcca atttcgcttt tgctggtttt attctgcatg
     1381 acgccgaaga tgtgatttca ccgatggaat tgcgtctgtt caactatctg gtcgagcgta
     1441 aagatctgat tcagatcccg gtgtatccgt tcgaacgcga atggacgcac ttcaccagca
     1501 tgacttacat tgatgagttt tcagagctgc atggcaaaga tgttccggtg cgtgaagccc
     1561 tcgccggaca agtgcccagc gcaggcgtcg gcacctgttt cagccgccgc gccgtgaccg
     1621 ctctgttagc tgacggtgac ggtattgctt tcgacgtgca gagtctgact gaagattacg
     1681 acattggttt ccgcctgaaa gagaaaggta tgacggaaat ttttgtccgt tttccggtgg
     1741 tggacgaagc caaagaacgc gagcagcgta aatttttaca gcacgcacgg acgtcaaaca
     1801 tgatctgcgt gcgcgaatat ttccccgata ccttttcgac tgcagttcga caaaaatctc
     1861 gctggatcat cggcattgtt ttccagggct ttaaaaccca caaatggacc tccagcctga
     1921 cgctgaacta ctttctctgg cgcgaccgca aaggggcaat cagtaacttt gtcagcttcc
     1981 tcgcgatgct ggtgatgctc cagcttttgc tgttgctggc gtatgaaagt ttgtggcccg
     2041 atgcctggca tttcctttct atttttagcg gcagcgcatg gttaatgacc ctgctgtggc
     2101 taaactttgg cttgatgttt aaccgcatcg tgcagcgggt gattttcgtc actggctact
     2161 acggtctgac gcaggggctg ctatctgtcc tgcgtctttt ctggggcaac ctgattaact
     2221 tcatggccaa ctggcgcgcg ctaaaacagg tacttcaaca cggcgatcca cgtcgcgtcg
     2281 cgtgggataa aacaacgcat gacttcccca gcgtgactgg cgatacccgc tcgttgcgcc
     2341 cgttaggcca aattctgctg gaaaatcagg tcatcactga agaacaactc gatacagcac
     2401 tgcgtaatcg cgtcgaaggt ctacgcctgg gcggttcaat gctgatgcag gggctgatta
     2461 gcgccgagca gctggcacag gcgctggcag agcaaaacgg cgtggcgtgg gaatccatcg
     2521 atgcctggca gatcccgtcc tcgctgattg ccgaaatgcc agcctccgta gcgctgcatt
     2581 atgcagtact gccgctgcgt ctggaaaatg atgagttaat tgtcggcagt gaagatggta
     2641 ttgaccctgt ttcgctggcg gccctgacgc gtaaagtcgg acgcaaagtg cgttacgtca
     2701 ttgttctgcg gggacaaatt gtcacggggt tacgtcactg gtatgcacgc cgacgcggtc
     2761 acgatccgcg ggcaatgttg tacaatgcgg ttcagcatca gtggctcacg gaacagcaga
     2821 ccggtgaaat ctggcggcaa tatgtgccgc atcagttcct gttcgccgaa atactgacca
     2881 cgctcggtca tattaatcgt tcagcaatta acgtgttgtt attgcgccat gaacgcagtt
     2941 ctctgccgct cggcaagttt ttggtcaccg aaggcgttat cagccaggaa acgttggatc
     3001 gcgtcctgac aattcaacgc gaattacaag tttcgatgca atcactatta ctcaaagcag
     3061 gtttaaacac agaacaggtt gcgcaactgg agtccgaaaa tgaaggagaa taaccttaat
     3121 cgcgtcatcg gatggtctgg tttactgctg acgtctttat tgagtaccag cgcactcgca
     3181 gacaatatcg gcaccagcgc agaagagctg gggctgagcg attatcgcca ttttgttatt
     3241 tatccccgtc tcgataaggc gctgaaggca cagaaaaata acgacgaagc aaccgccatc
     3301 cgcgaatttg aatatataca ccagcaggtg ccggataata ttccgctgac tttatacctt
     3361 gcggaagcct atcgccattt tggtcatgat gaccgggcgc ggctgttgct ggaggatcaa
     3421 ctgaaacgtc acccaggaga tgcccgactt gagcgcagtc tggcggctat tccagttgaa
     3481 gtgaaaagcg ttacgactgt tgaagaactg cttgcccagc aaaaagcgtg cgatgctgcg
     3541 ccgaccctgc gttgtcgcag tgaagtcggg cagaatgccc tgcggctggc acagttacct
     3601 gtcgccagag cgcaactgaa cgatgcgacg tttgctgcat cgccggaagg aaaaacgctg
     3661 cgaaccgatc tgctgcaacg ggcaatctac ctgaaacaat ggtcccaggc agatacgcta
     3721 tacaatgaag cacgccagca gaacacatta agcgcggcag aacgccgtca gtggtttgac
     3781 gtgcttcttg ccgggcagct ggacgatcgg atcctggcac tgcaatcaca ggggatcttc
     3841 accgatcctc agtcatatat tacttacgcg accgcgctgg cttatcgtgg cgaaaaagca
     3901 cgcctccagc attatctcat tgaaaataag ccgctgttta ccacggacgc acaagagaaa
     3961 agttggctct atctgttatc taaatacagc gccaaccccg ttcaggcatt ggcgaattat
     4021 acggtgcagt ttgccgataa ccgccagtat gttgttggcg cgacgctacc ggtgctgtta
     4081 aaagaaggtc agtacgacgc agcgcaaaaa ctgctcgcca ccctccccgc caatgaaatg
     4141 cttgaggagc gttatgctgt cagcgtggcg acccgtaaca aggctgaatc tctgcgtctg
     4201 gcacgattgc tgtatcagca agaaccggca aatcttaccc gcctagatca actgacctgg
     4261 caactgatgc agaacgagca gtcacgcgaa gctgccgatt tattgctgca acgctatcct
     4321 ttccagggcg atgcgcgtgt cagccagact ttaatggcgc gactggcgtc tctgctggaa
     4381 agtcatcctt acctggcaac tccagcgaag gtggcgattt tatcaaaacc cttgccgctg
     4441 gcggcgcaac gtcagtggca aagccagttg ccgggtattg cagataattg cccggcaata
     4501 gttcgcttgc tgggcgatat gtcgccttcc tacgatgccg ccgcctggaa ccgtctggca
     4561 aagtgttatc gggacacgct acccggtgtg gccttgtatg catggcttca ggccgaacaa
     4621 cgacaaccga acgcctggca acatcgtgcg gtagcctatc aggcgtatca ggttgaggac
     4681 tacgccaccg cactggcggc ctggcagaaa atcagtcttc acgacatgag caatgaggat
     4741 ctgcttgctg ctgccaatac cgcccaggcg gcaggaaatg gtgcagctcg cgatcgctgg
     4801 ctacaacagg cagaacaacg tggactggga aacaatgccc tctactggtg gctgcatgcg
     4861 caacgttaca ttcctggtca gccggaactc gcactgagcg atctcacgcg ctcaatcaat
     4921 attgcgcctt ctgccaacgc ttacgttgcg cgggcgacaa tttatcgcca acgtcataat
     4981 gtcccggcgg cggtgagtga tttgcgcgcc gcgctggaac tggaaccgaa taatagcaac
     5041 atccaggcag cgcttggtta cgccttgtgg gatagcggtg atatcgcaca gtcgcgggaa
     5101 atgctcgaac aggcgcataa agggcttccg gacgatccgg cactgatccg acaactggcc
     5161 tacgtgaacc agcgtctgga tgacatgcct gcgacgcagc actacgcccg gctggtgatt
     5221 gatgacattg ataatcaggc gctgataacc ccactgaccc cagagcaaaa tcagcaacgc
     5281 ttcaatttcc gccgtttgca tgaggaggtc ggtcgccgct ggacgttcag tttcgattct
     5341 tctatcggct tgcgttccgg ggcaatgagc accgctaaca ataatgtcgg cggcgcagcg
     5401 ccagggaaaa gctatcgtag ctacgggcaa ctggaagccg agtaccgcat cggacgcaat
     5461 atgctgctgg aaggcgacct gctctcagtt tatagccgcg tctttgccga taccggagaa
     5521 aacggggtga tgatgccggt gaaaaatccg atgtccggca ccggtctgcg ctggaagccg
     5581 ctgcgcgatc agatcttttt cctcgccgtc gaacagcagt tgccgctgaa cggccaaaat
     5641 ggcgcatccg ataccatgct gcgcgccagc gcctcattct ttaatggcgg caaatacagc
     5701 gacgaatggc acccgaacgg ttcaggctgg tttgcccaaa acctgtacct cgatgcggcg
     5761 caatatatcc gccaggatat tcaggcgtgg acggcagatt atcgcgtcag ctggcatcag
     5821 aaggtagcta acggacagac tattgagcct tacgctcacg ttcaggacaa cggctatcgt
     5881 gataaaggca ctcagggcgc gcagcttggc ggagtcgggg tccgctggaa tatctggacc
     5941 ggcgagacgc actacgacgc ctggccgcac aaagtcagtc tcggcgtcga gtatcaacat
     6001 acctttaagg cgattaatca acgtaacgga gagcgcaaca acgcgtttct caccattgga
     6061 gtgcactggt aaatgcgtaa gttcattttc gtattgctga cactgctttt ggtcagccct
     6121 ttttcctttg cgatgaaagg tattatctgg caaccacaaa accgagatag tcaggttacc
     6181 gatacccagt ggcaggggct gatgagtcag ttacgtttgc aaggcttcga taccctggtt
     6241 ttgcaatgga cccgttacgg cgatgcattc acccagccag aacagcgcgc gttattgttt
     6301 aagcgtgccg cagctgcgca acaggctggc ctgaagctga ttgtcgggct gaacgccgat
     6361 ccggaatttt ttatgcacca gaaacagtcg tccgcagcgc tggaaagcta tcttaatcgc
     6421 ctgctggctg ccgatctcca gcaagccaga ttatggagcg ccgcgcctgg cgtaacgccg
     6481 gatggctggt acatcagcgc ggaaattgac gacctgaact ggcgcagcga agctgcccgt
     6541 cagcctttgc taacatggtt aaacaacgcg cagcggctga ttagcgatgt ttcagcaaaa
     6601 ccggtttata tcagtagttt tttcgccgga aacatgtcgc ccgatggcta tcgccaactg
     6661 ctggaacaag ttaaagcaac cggcgttaat gtctgggtac aggatggcag cggcgtggat
     6721 aaacttaccg ctgaacagcg tgaacgttat ctacaggcca gcgccgattg ccaaagtccc
     6781 gcccctgcca gcggcattgt ttatgaactg tttgtcgccg gcaaaggcaa aacctttaca
     6841 gcgaaaccga aaccggatgc agagatcgcc tcgctgttag cgaaacgttc ctcttgcggt
     6901 aaagacactc tctatttctc tctgcgctat ttgcccgtcg cgcacggcat tctcgagtat
     6961 taaatctcct ccaggtaagt cgggtacgac ctggcttacc tctttcgctc tcgtcagaat
     7021 tccatcaaga ttaattgcta aaaagcgtgc taaagaaaga agatcgttat cggcgaacgg
     7081 gccgatcact catgcaggaa gcatagatcc tttgagcgga gtttctgata tctgaggagt
     7141 gcgaaatgca attgagcagc agtgaacctt gcgtggtgat cctgaccgaa aaagaggtag
     7201 aggtaagcgt caataaccat gctacgttta cccttccgaa aaactacctg gccgccttcg
     7261 cgtgcaacaa taacgtcatt gaactctcaa cgttaaatca cgtattaatc ccccacatca
     7321 accgtaacat catcaacgat tatctgttgt ttttaaataa gaacttaacc tgtgtaaagc
     7381 cctggtcgcg gctggcaacc ccggttatcg cttgtcatag ccgtacaccg gaagtgttcc
     7441 ggctagccgc caaccacagc aagcagcaac ccagcaaacc ctgcgaagcg gagttgacgc
     7501 gcgcattact ttttaccgta ttgtctaact ttcttgagca atcgcggttt atagccctac
     7561 tgatgtatat cttacgcagc agcgtccgcg acagcgtctg ccgcattatt caaagcgata
     7621 ttcagcatta ctggaatctg cgaattgtcg ccagttcgct atgtttaagc cccagcctac
     7681 tcaaaaagaa attaaaaaac gaaaatacca gctatagcca gattgtcaca gagtgtcgta
     7741 tgcgttacgc cgtacagatg ttattgatgg ataacaaaaa tatcactcag gtggcgcaat
     7801 tatgtggcta tagcagcacg tcgtacttta tctctgtttt taaggcgttt tacggcctga
     7861 caccgttgaa ttatctcgcc aaacagcgac aaaaagtgat gtggtgaagg gcaaagcgga
     7921 aacggataag acgggcataa atgaagaaga aatggcgcgc cctgcaggat tcgaacctgc
     7981 ggcccacgac ttagaagttc ctagaacgac attttaagtc aacaaattac cgcgccatct
     8041 ctgcgctcac acgtcccact acctcaaaac atgtaaagcc ttgcaagcca ttgtgaggcc
     8101 ttatgtgtct cagttttgtc cctctttttt gtactaaaaa acatagcaat tgaggataaa
     8161 cctcatgcta ttttcgctta tatgccttta aaggcatggc gcttaaatag ataaaaacac
     8221 cacaaaagca taaaaaacca cacagtaaaa ccgcaacata aaacaataac agataattaa
     8281 accaaaaaca gatagcgcat tgtgataatc attcaatact aaacaaaata taaacagtgg
     8341 agcaatatgt gattgactca ttaagttaga tataaaaaat acatattcaa tcattaaaac
     8401 gattgaatgg agaactttta tgcgggcgaa acttctggga atagttctga caacccctat
     8461 tgcgatcagc tcttttgctt ctaccgagac tatatcgttt actcctgaca acataaatgc
     8521 ggacattagt cttggaactc tgagcggaaa aacaaaagag cgcgtttatc tagctgaaga
     8581 aggaggccga aaagtcagtc aactcgactg gaaattcaat aacgctgcaa ttattaaagg
     8641 tgcaattaat tgggatttga tgccccagat atctattggg gctgcgggct ggactactct
     8701 cggcagccga ggtggcaata tggtcgatca ggactggatg gattccagta accccggaac
     8761 ctggacagat gaaagtaggc accctgatac acaactcaat tatgccaacg aatttgatct
     8821 gaatatcaaa ggctggctcc tcaacgaacc caattaccgc ctgggactca tggccggata
     8881 tcaggaaagc cgttatagct ttacagccag aggtggttcc tatatctaca gttctgagga
     8941 gggattcaga gatgatatcg gctccttccc gaatggagaa agagcaatcg gctacaaaca
     9001 acgttttaaa atgccctaca ttggcttgac tggaagttat cgttatgaag attttgagct
     9061 aggtggcaca tttaaataca gcggctgggt ggaagcatct gataacgatg agcactatga
     9121 cccaggaaaa agaatcactt atcgcagtaa agtcaaagac caaaattact attctgttgc
     9181 agtcaatgca ggttattacg taacacctaa cgcaaaagtt tatgttgaag gcgcatggaa
     9241 tcgggttacg aataaaaaag gtaatacttc actttatgat cacaatgata acacttcaga
     9301 ctacagcaaa aatggtgcag gcatagaaaa ctataacttc atcactactg ctggtcttaa
     9361 gtacaccttt taacgaagtt aaccagattt tctccccggt ggtattgtat aaccccgggg
     9421 aatttgttta cagttacaag taggcaatat ccactaattt tgtaagcatt taacctatgt
     9481 cataacgtgt aatctcttac cgcaacacaa gacctcaata tattcaaatc ttatctttat
     9541 tccgatattc agagacacaa tgcgaaaaat aattactcat ttcaaagttg ttttaacgtt
     9601 acttctacca gtaaccgtat ctgcccagca gatacagtgg caatcctgta tggccagtca
     9661 attcaaccac tggtttggtg aggaaaaacc gtctcctgac ttactatgtg gttatttgtc
     9721 tgttccatta aaatatacag acacaggcgg agatgcttct tatgaaaaaa aatcacaagt
     9781 caaactagcg ttgacaaaat tgccggcaaa aagcaagcat aaaggaagta tcctgataat
     9841 aagtggtggt cccgggttac caggcataaa tccttatatt aactttgact ggccagtcac
     9901 aaatcttcgt gagtcatggg atattattgg atttgatcct cgaggcgtcg gacagtccac
     9961 tccgacaata aactgccggc aatcagatac agagactcag gaaaacataa ccgaaaagca
    10021 acaagtatta aataaaatta atgcctgtat ccataatacc ggagccgaag tcattcgcca
    10081 tatcggctct aacgaggctg tatacgatat tgatcgtatt aggcaagcct tgggggataa
    10141 acaactgaca gccgtggcgt attcgtatgg aactcaaatt gcagccttat atgcagaacg
    10201 ttttccctac aacgtaagat ctatcgttct tgatggagtc gtcgatatcg atgacctgga
    10261 ggacaacttc acatggcaac tcaaacaggc acagagttat caggaaacgt ttgatcgctt
    10321 tgcatcctgg tgtgcgcgta caaaaagttg cccgctttct tcagacagag ataaggcaat
    10381 aactcagttc catgagctat tatcaaaatt acatcacaaa cctttattag acagtaagag
    10441 agaaaatata tcttcagatg aactcatatc attaacaaca gaccttctgc tatggcgttc
    10501 atcatggcca acccttgcaa ctgccatacg ccagttctct caggggattg tcagtaatga
    10561 aattgaaact gcgctcagtg ctccgatagc ctcagaagag tcaagcgatg cttcgggggt
    10621 aatcctctgt gtagatcagg gggatgagca attaacacca gaagagcgaa aatcccgaaa
    10681 agacgctctt gcgaatgcct tcccggctat taactttgac aatggacgtt ccgattcacc
    10741 tgatttttgt gaattatggc caatacatag cgacctgaac aaaactcgcc tgaaaaatac
    10801 tgttctgccc tctggtttac tgtttatagc acacaaatac gacccaacaa cgccctggat
    10861 taatgcccgt aagatggcag agaaattttc cagcccgtta ctaacaataa atggtgatgg
    10921 gcatacatta gctctcaccg gagttaattt atgtgtagat aaagcagttg tacatcacct
    10981 gatcactcca c
//
LOCUS       DAZGJW010000148        10933 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZGJW010000148 DAZGJW010000000
VERSION     DAZGJW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.148"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            153..803
                     /gene="csgD"
                     /locus_tag="ACHKIK_004033"
     CDS             153..803
                     /gene="csgD"
                     /locus_tag="ACHKIK_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO9085106.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            808..1197
                     /gene="csgE"
                     /locus_tag="ACHKIK_004034"
     CDS             808..1197
                     /gene="csgE"
                     /locus_tag="ACHKIK_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO9085107.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1222..1638
                     /gene="csgF"
                     /locus_tag="ACHKIK_004035"
     CDS             1222..1638
                     /gene="csgF"
                     /locus_tag="ACHKIK_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO9085108.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1665..2498
                     /gene="csgG"
                     /locus_tag="ACHKIK_004036"
     CDS             1665..2498
                     /gene="csgG"
                     /locus_tag="ACHKIK_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO9085109.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2562..3053)
                     /gene="ycdZ"
                     /locus_tag="ACHKIK_004037"
     CDS             complement(2562..3053)
                     /gene="ycdZ"
                     /locus_tag="ACHKIK_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO9085110.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGSFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3155..3709)
                     /gene="ycdY"
                     /locus_tag="ACHKIK_004038"
     CDS             complement(3155..3709)
                     /gene="ycdY"
                     /locus_tag="ACHKIK_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO9085111.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3733..4470)
                     /gene="ycdX"
                     /locus_tag="ACHKIK_004039"
     CDS             complement(3733..4470)
                     /gene="ycdX"
                     /locus_tag="ACHKIK_004039"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO9085112.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4525..5463)
                     /gene="ghrA"
                     /locus_tag="ACHKIK_004040"
     CDS             complement(4525..5463)
                     /gene="ghrA"
                     /locus_tag="ACHKIK_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO9085113.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGADVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5697..5784
                     /locus_tag="ACHKIK_004041"
     tRNA            5697..5784
                     /locus_tag="ACHKIK_004041"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5731..5733,aa:Ser,seq:gga)
     gene            complement(5934..6776)
                     /locus_tag="ACHKIK_004042"
     CDS             complement(5934..6776)
                     /locus_tag="ACHKIK_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO9085114.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6861..7058)
                     /locus_tag="ACHKIK_004043"
     CDS             complement(6861..7058)
                     /locus_tag="ACHKIK_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO9085115.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7070..7561)
                     /locus_tag="ACHKIK_004044"
     CDS             complement(7070..7561)
                     /locus_tag="ACHKIK_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO9085116.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7558..7932)
                     /locus_tag="ACHKIK_004045"
     CDS             complement(7558..7932)
                     /locus_tag="ACHKIK_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO9085117.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(7979..8353)
                     /locus_tag="ACHKIK_004046"
     CDS             complement(7979..8353)
                     /locus_tag="ACHKIK_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO9085118.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPALATTS"
     gene            complement(8516..8737)
                     /locus_tag="ACHKIK_004047"
     CDS             complement(8516..8737)
                     /locus_tag="ACHKIK_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO9085119.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8800..9276)
                     /locus_tag="ACHKIK_004048"
     CDS             complement(8800..9276)
                     /locus_tag="ACHKIK_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO9085120.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9292..9777)
                     /locus_tag="ACHKIK_004049"
     CDS             complement(9292..9777)
                     /locus_tag="ACHKIK_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9085121.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPLQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9868..10686)
                     /locus_tag="ACHKIK_004050"
     CDS             complement(9868..10686)
                     /locus_tag="ACHKIK_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO9085122.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
BASE COUNT         2765 a         2727 c         2721 g         2720 t
ORIGIN      
        1 aaatcagatg taatccatta gttttatatt ttacccattt agggctgatt tattactaca
       61 cacagcagtg caacatctgt cagtacttct ggtgcttcta ttttagaggc agctgtcagg
      121 tgtgcgatca ataaaaaaag cggggtttca tcatgtttaa tgaagtccat agtattcatg
      181 gtcatacatt attgttgatc actaaaccgt ctttgcaagc gacagctctc ttgcagcacc
      241 ttaaacaatc gctggcaatt acaggaaaat tacataatat tcaacgttct ctggacgata
      301 tatcttcagg ctctattatt cttctggata tgatggaagc agataaaaag cttatccatt
      361 attggcagga tactttgagc aggaaaaaca acaatatcaa aatattgttg ctaaatacgc
      421 ctgaagatta cccgtaccgc gacattgaaa actggcctca tatcaacggc gttttttatg
      481 ccatggagga tcaagaacgt gttgtcaatg ggttgcaagg cgtcctgcgc ggcgaatgct
      541 actttacgca aaagcttgcc agttacctga ttacacattc tggtaactat cgttataaca
      601 gcacggaatc agccctcctt actcatcggg aaaaagagat cctgaataag ctgcgtatcg
      661 gcgcgtctaa taacgagatc gctcgttcgt tgttcatcag cgaaaatacg gttaaaacgc
      721 atctttataa tcttttcaag aagatagccg taaaaaaccg gacacaagcg gtttcctggg
      781 caaacgataa cctcaggcga taaagccatg aaacgttatt tacgctggat tgtggcggca
      841 gaatttctgt tcgccgcagg gaatcttcac gccgttgagg tagaagtccc gggattgtta
      901 actgaccata ctgtttcatc tattggccat gatttttacc gagcctttag tgataaatgg
      961 gaaagtgact atacgggtaa cttaacgatt aatgaaaggc ccagtgcacg atggggaagc
     1021 tggatcacta taacggtcaa tcaggacgtt attttccaga cttttttatt tccgttgaaa
     1081 agagacttcg agaaaactgt cgtctttgca ctgattcaaa ctgaagaagc actaaatcgt
     1141 cgccagataa atcaggcgtt attaagtacg ggcgatttgg cgcatgatga attctaaata
     1201 aaaaattgtt cggaggctgc aatgcgtgtc aaacatgcag tagttctact catgcttatt
     1261 tcgccattaa gttgggctgg aaccatgact ttccagttcc gtaatccaaa ctttggtggt
     1321 aacccaaata atggcgcttt tttattaaat agcgctcagg cccaaaactc ttataaagat
     1381 ccgagctata acgatgactt tggtattgaa acaccctcag cgttagataa ctttactcag
     1441 gccatccagt cacaaatttt aggtgggcta ctgtcgaata ttaataccgg taaaccgggc
     1501 cgcatggtga ccaacgatta tattgttgat attgctaacc gcgatggtca attgcagttg
     1561 aacgtgacag atcgtaaaac cggacaaacc tcgaccatcc aggtttcggg tttacaaaat
     1621 aactcaaccg atttttaagc cccagcttca taaggaaaat aatcatgcag cgcttatttc
     1681 ttttggttgc cgtcatgtta ctgagcggat gcttaaccgc cccgcctaaa gaagccgcca
     1741 gaccgacatt aatgcctcga gctcagagct acaaagattt gacccatctg ccagcgccga
     1801 cgggtaagat ctttgtttcg gtatacaaca ttcaggacga aaccgggcaa tttaaaccct
     1861 acccggcaag taacttctcc actgctgttc cgcaaagcgc cacggcaatg ctggtcacgg
     1921 cattgaaaga ttctcgctgg tttataccgc tggagcgcca gggcttacaa aacctgctta
     1981 acgagcgcaa gattattcgt gcggcacaag aaaacggcac ggttgccatt aataaccgaa
     2041 tcccgctgca atctttaacg gcggcaaata ttatggttga aggttcgatt atcggttatg
     2101 aaagcaacgt taaatcgggc ggtgttgggg caagatattt tgggatcggt gcagacacgc
     2161 aataccagct cgatcagatt gccgtgaacc tgcgcgtcgt caatgtgagt accggtgaga
     2221 tcctctcctc ggtgaacacc agtaagacga tactttccta tgaagttcag gccggggttt
     2281 tccgctttat tgactaccag cgcttgctgg aaggggaagt gggttacacc tcaaacgaac
     2341 ccgttatgct gtgtctgatg tcggctatcg aaacaggggt cattttcctg attaatgatg
     2401 gtatcgaccg tggtctgtgg gatttgcaga acaaagcaga acggcagaac gacattctgg
     2461 tgaaataccg ccatatgtcg gttccaccgg aatcctgaca gataaaaaag ccggaattat
     2521 catccggcat tttactgttt aagcgaaagc gtgagtgccc ctcacgcttt atttttgatt
     2581 tcctgctcac ggtgagccgt ctttgcacta cgcgccgcca gccacaggcc gctgtttttc
     2641 attgcgtaac caaataccag cccaagcgcc agcgaaggta acaccagttt ccagtcaccc
     2701 tgcccggcaa acgtcgcgca tgcgcctata aacgaccccg gaacaaatga aagcagcagc
     2761 tgtttggcct gaatacacat cagaaacgcg acaataccgg ttatgacata accgagaatt
     2821 tccagatgtg gtgccagtgc actaccgtaa ataatgacca tcgcccacac cacgccacta
     2881 agcagggttg cagcggagat cgccagccct ttcagcccac cttgcgggca ggcaaagtag
     2941 gccgtacagc ccaggaagcc cgcccagctt agtaagccca gggaaacagc cacccatccc
     3001 cagataccgg agagaatgcc tgttgtgatt gcaatagaga gaagtatgtt catgccgcgc
     3061 atgatagcag aaaacgcggc catgaatgag atcgagcaca cattttatgc aacaacaaat
     3121 tatctgttgc agttatgagt gacgatgatc acatttactc ttcagaatct tcttccagct
     3181 cgtcccacat tgcactaatg gcatcacggg ttagcggtgc catggtgcgc cagaaaggcg
     3241 tggttgcatg ggcctccact ttgccgagga acgcaccaca ccagggtaac agatactcac
     3301 tgaacagtgt ttccagtgct tcgctctcat cttccgttga ctgatcttcc agccaggaag
     3361 ccgcaagcag caatgtgccg atgtgatcgg ctggcgtatc cgccagtggc atccctcgtt
     3421 cggaaagaaa agcgcgcact tccgcttccg ttgcgtcctc aacccatgcg ctacgatatg
     3481 gcggcacggc acattcatcg ccgataaaca acgcattgta atcggcagag acttgcgcca
     3541 tatcacagct tttctgtaaa cgcgtcagta actcatcctg ctccagtggc cagttcgcag
     3601 ccagtttccc ctcacgaatc agggtaaaca gcggcaccag taaaggatct tgcggttggc
     3661 ggtaatagag cgaacccagc acacgacaga ggatagaaaa ctcgttcatt aatatatttc
     3721 cattactagt aattaaaggt ctgcaaattc cgcaatcggt gccataccgc gagattcaag
     3781 gaagttcagt aagcggcgcg gcgaaacatt caaaatgcgc tctggcggaa aatctaccgc
     3841 gtcgaggatt ttaagacact cttcaaattc ccccatggta aacgcggtgt gagaatccga
     3901 gcctaatgcc acccaacctc cagcatcacg taccgctgcg gctaccgcac gacagttgtc
     3961 ttcactgccc ttacgtgagt gtaaaaatga agaattattg atttccagcg ccacctgatg
     4021 tttcgcggct gcttcagcaa cggctttcac atctatttca tatttgggat ttccgggatg
     4081 gcttattata tgcacattgc cgctggcgat agtggcgatc atcgcttgtg tattggtcgc
     4141 tttgtcatgt ggtgcaaaaa ccggctcatg aaaaccggca ataatcagat ccagcgagtc
     4201 aaacatttta ccgctgcaat caatttcacc atcaacattt ttaatgttag cttcgatgcc
     4261 gcgcaggatc cctaccccat caaccactcg cggccagata cgcatgttaa tgaagtgcca
     4321 gtgatgcgga gcatcttcca tatccgggcc atgatcggtg atcgcaaaaa gtttaatgcc
     4381 cttttgtttg gcctgggcaa tgtaatcact caatgtgcta tatgcatgtg tgctggcaac
     4441 ggtatgcata tgaaggtcga cgggatacat aactctctcc tgtattcaat tcccgccaag
     4501 gatagcagga atcctgatgc tttattagta gccgcgtgcg cggtcgactt gcccgcagac
     4561 cctctcccct ttttcgagct gggcaatggt gcgagaaatg tactccacag cttcagcggg
     4621 acgggtaatc gcggcgacat gtggtgttat cgtcacgcgt ggatgttgcc agagcggact
     4681 ttcaggcggt aagggttcac gattaaaaac atccaacatt gcgcctttaa ctttgccgct
     4741 atccagcgcc gcgagcaggt catcttccac aacatgaaca ccacgcgcca ggttgaggag
     4801 atacgcgcca tccggtaatt tttcgagtaa ttgttgatta ataatgccga cggtttcagg
     4861 ggtattcggt aacaaattaa tcaatacccg acattggctc agaaatgcag acagttcttc
     4921 ccgtccggca aagctttgca cgccaggcca cgatttacgg gttcgactcc agcaacgcag
     4981 cggaaagcgc caggtttgca gactctgagc aactttactg cccagtacgc ctgcgcccaa
     5041 aatgccgatg gtaaaatctt cccgatgata ttcaggcagc ggttgccaat gcgaactatt
     5101 ttgctggatg cgataatcgt caaaacgtcg aaaccaatgc agcacctgac tgacagcata
     5161 ttcctgcatt tgctcgccca taccggtatc ttccaggcga aaaagtggaa cagaagggtt
     5221 cagcatttca gggtgtgcct gtagcttgct caaaatagaa tcaacatcgg ccccgagtgc
     5281 gaacaccgct ttaagatcgc gccctgccag catttcaaca ggaggatgcc agactaaagc
     5341 ataatcagca gagtcattat ctccgctttt ccacgctctg actcttgcct gaggaatagc
     5401 tttgcgcagt gcctcaatcc accattgggt atcgaacgtt gggtgataaa agatgatatc
     5461 catactgact cccgaaaagc gttgtgcgaa atttattcgc acttatcgtt atgatctaca
     5521 aaggccacca gcataacaaa tccgtggtcg gtggcaaaaa aagcagattt cgcttattaa
     5581 aaccacacat tgattgaagt ttgaataaac gcgcgatttt ttcaaaaagt ttgttgacct
     5641 caggtcatga tttccctaaa ttagcgcccg ttccagcaag acaggaacga caatttggtg
     5701 aggtgtccga gtggctgaag gagcacgcct ggaaagtgtg tatacggcaa cgtatcgggg
     5761 gttcgaatcc ccccctcacc gccagattat aagcgcatgt tattgtttaa catgcgcttt
     5821 tttattttcc cttccagaca gtatgccatc gggtatgcca tctgaaaccg gtttatatgc
     5881 cccgcgatca tatcttatcg gacaatttcc gatctttgag catactggca aagctatact
     5941 gccagcatat caggatagtg ttttgcgata atatcattca gtctgtcaac cagttctggt
     6001 tttctgaacg tgatatgcgc ggagcccttc ttaaagtatc tgatgctgaa catctcatct
     6061 tcatagctct ctttaccctg aacagactga atgtgatcat ccagacgaag ggtgatgttt
     6121 ccccggttgt cggggattgg tttaccgctg aacaggtgca acattctttc cagatcggcg
     6181 agtcggtcag cctgttgtcc ggtgataaga tgaaatcccc accggtccca tctcaccaga
     6241 ttgttgacga taattttact gccaaattta caggggcaat tggttttgta attccagttc
     6301 agccctctga agacgttgat cacccctcgc tcaaacacct catccttatt ctggtgcagt
     6361 tgttcaaatg tgctcagaat gttcgcttcg ctgatttccg gaaaattatc gtactccagt
     6421 gaccggtacc atgtatcacg ggcctgcgta tccattaatg acagcatccc ggaacgttgc
     6481 atcaggtcac gccagatttc gcgatcaaga ttccgggtaa tggctttcat tgctgtttcg
     6541 ggtttctcca tcagccagca gccgcagcga aaatcctgtt tcatggccca gtccggggca
     6601 gttttacctc cgatgctttc cgtcagcacc gagatttcag ccagttgtcg gataagtgtg
     6661 tcgatttgca acagtgtact ctttcgtaca gccagaatgt ggcgaatgct ggttgagcag
     6721 ataacgtccg tatgcccgtt gaccacttcc ggacgggaga ttgtgatatc tgacataatc
     6781 gatattcctt tttgcagata aatgaaaacg ccagccctgt gcaggctggc gtcagaggaa
     6841 tgaagtctgg tgagtgagct tcaccggttc tggtgcagaa gctgcagatg acgcagggta
     6901 cggacatcct cccgggcctg ttcgatacag ggcaacagtg ctgctgaatc cgttttatcc
     6961 tcatcgttgt caaagataat tcccgatccg cagtcgatat tgtcctgcag ccacgcaatc
     7021 agaatatcca gcgctgtttc cgtggttaat gatttcatgt tgttcctggt taccagtcga
     7081 aagtgggtaa acccggcagg cattcagcgc tggcatccag atgaatgaga ctgacgccat
     7141 aacgccggat gagtgtgacg accagacgac ggaacgtttt tgacagcccc aggcgtttaa
     7201 gccgcagaac cgggtatgac cacgcatcgg tacgtaacag atatccgcta cctgtaaaat
     7261 gaatccattc tgattcaccg aagtcactgg tctggtgtga cagcgagtac aaccaggcgt
     7321 tgtccgtttc cgtgatatgt gcggtactgc agcgtatgcc ggtaagggta gcaaatggtg
     7381 gcgtgcattc tgctgaggtc tggtcagatt catcaacaac acgaagggag tagccgttat
     7441 cgcagaccat gttaatcagt tcagcgagct caataccatc gacgtcaacg acaatgcgcc
     7501 ccatgttcag tgcctgtacg ttaacgctgt cggcttccag tgtcagggcc agtttcatca
     7561 tttcgcctcc ggatacttac ccaaggtaat gttatttacc gttctgtaat tgtcgcgggt
     7621 catcaggccg gtcgcccggc gagcccggag gatatcaata ctgtttatta actgagagcg
     7681 gggacaggtg ctgaatcccg gctggtcggt acgcaccagc gcgtattttt ccacgagaaa
     7741 attaactgca tcacacagtg aaatgcctgc ctcaatatgc tgctcaatca cacgttcatc
     7801 ggcaaacggt gtgtcattca gtgtgaggcc ataatgctgg tccagcagtc ggatgagcag
     7861 tgtctgccag atggtgacgg gagacgggca gcgagatacc tcccgtacat gtgtgtcgga
     7921 taatgttttc atcttaagtt ctctgtaact ggtagtgcta aaggtgagag cttcggggtt
     7981 atgaggtggt tgcgagtgct ggtgtcggat aaacagccag ataaacgtaa ccacaggagc
     8041 cgagggtgtc ggcttcgcag gtcagccctt ttgcattcag cgtgacggta tgctgatatc
     8101 ggggattcag ttcaccgctg gtgagcatga gttccagttg tttcatcagc agcggaaagg
     8161 cctggtccag atggtatgca tctgcgtcgc tgaagcggcc tctgataccg gcgcggtcgg
     8221 caaggtaatg caggcggtta ccctcttgca ccagacgagc gccaaaacag ggcctcactg
     8281 tgcagggaag cccccaccag gggcggtcgt gattgtcatc gggatgagtt atcccggaga
     8341 gtttgtctga cacgataaaa tcctctctga aaagtggtta agaatggttt aacgatgaca
     8401 ggtcagccgg cgcgctgacc ggtttcggtg aagatgtatg cattgatgag aatggcctca
     8461 tccacagcgg cgtctgaggt catctggtcg tactcgtttc ccgggtgacg gaacatcagt
     8521 tcagggtgac actcatcagc cggacatacg ggccaaaact gtccttacgg cgttctgcaa
     8581 acacggcaag aacgccggga atatcctgca cttcacgacc agtatacgct tcagcactgc
     8641 cgtgccagcg atacttaccg gtacagaacg gaaaaagacg ggatgtcgga tgttgttggt
     8701 gaatacgcat ggcttcacca cgggtgataa ttttcataat gggatacctc tgaagacaga
     8761 agataaaagt gaaaacaggt gtgatgtggt tgtgaagggt taaagcagac catgttcggc
     8821 aaaggagaaa acctggttgc caccgactat cagatggtcc ggcacccgga tatccaccag
     8881 gcccagtgcc tgtaccagac gttcggtgat aagccggtct gccttactgg gtgtgacttc
     8941 accggacggg tgattgtgcg ccagcaccac ggcagcggca ttgtggtaca gggcgcgttt
     9001 aatcacttcc cggggatgaa cttccgtacg gttgatggtg ccggtgaaga gggtttcacc
     9061 ggcaatcagc tgattctggt tgttcagata cagcacccgg aactcttccc gctccagtcc
     9121 cgccatgttc agaatcagcc attcccgtgc cgcacgggtg gaggtgaagg ccacgccggg
     9181 ttcatgaaga tggcggtcca gggtttgcag ggcccgcaga atgagactgc gctcgccggg
     9241 cgtcatctct ccgggcagaa aggaaagctg ctgcattgtc cctgcctcca ttcagtcaat
     9301 gatgcgcata atggcgctgc attccggatg ctgcagggcg taatcccgca gccggtaata
     9361 gtggaccgtc atggcataac actccgtacg acaggcatga tgactgtacg tcatcagaca
     9421 ggcagcaata ccggcggctt ccgggctcat ttcagcgcgg ttgccgttca tggcattgaa
     9481 cagtacccat gtttcgtcat catcgtcatc cggttcgggt gccataaatg ccccgccgtt
     9541 gttcagggtg tacagattcc agataccacc gcagtagtct tcgcaaagac ggtccatcca
     9601 gccgaagaca cggggctcca gggtcaccca ctgtggaatg aggccaaaat gctgcggcca
     9661 gaagctgatg cgctgttcat cagggacggg agtggcaacc agctgaagtg ggttattccc
     9721 tgatgcagtg gttacggaaa cagaaggtgt ggtggaatta tgcgaaacgg ttgtcatgag
     9781 gttattcctt ataaaaagta aatgaatgga agaagccccg gggcaggaac agatgtgagt
     9841 caggactgca ctttcagtga aacggcatca gcgcatgctc tccagcagcg tttctgccat
     9901 cacccacaat gcgcgattga gcttaatgtc ggtatcaatg ctgtgaatgg cccgggtatg
     9961 gatacgtttt ccttttgcac tgcgaccgga aatcccgcct ttcagcatat tctcctggat
    10021 ggtctgataa gcactccaca ggtccttacc gtaatcctcc cggcgtcgtg gtgtcagaat
    10081 gtcggcggtg gtgacgggct gatgttcgtc accataacgg taagtcagtg ccgcctgtgc
    10141 cagcgcctgg cgtgccggtg gcggcaggac cagcgactgc atggcatcac gcttctcctc
    10201 tatccggtca aaaacgccca ccacctcgta agccccttca atgactctgt ccaccacatt
    10261 tccccggtgt ggaacacgca attctcccag agactgaccg cagaccagtc cattggtaca
    10321 aatggcacga aaatatccgg gtaacatctg gtagctggat gaaccgtcat gggagttgag
    10381 cagaataatt tcagggacat gctgaccggt tatctgtccg gcccgccgca gacgcagcat
    10441 gtgttttgtg tattcccggc ggctctggtc gcgcacgcgg gtctggcagg caaagaacgg
    10501 ctgaaagcct tcctgctgca gattttccag gacggtgata gtgggaatgt acgcatagtg
    10561 ttcactgcga gaggtatgtc ggttttcccc aaaaatactc ggtacatggc gcatcagttc
    10621 ttcgtgtgtc agcggacgat cacgacgtat ctggttcgca taaccaaaac gactggctaa
    10681 tctcatgatt tgctccttat gaatgattaa gcgatataaa tgaaaaagcc gcgtctcccg
    10741 gagaagacgc ggcctgatgg gtgaaatgaa tgaggtttat tgtctgagaa gcccttcact
    10801 ggcgaaccac gtattaagct gtattccggc atcgccagtg caactgacct tcagcattgc
    10861 ggataacccg ccggaaatat gttcactggt tgtttttgta tcttccccca aaattaaata
    10921 aattcatctt aac
//
LOCUS       DAZGJW010000149        10860 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZGJW010000149 DAZGJW010000000
VERSION     DAZGJW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.149"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            483..2411
                     /gene="thrS"
                     /locus_tag="ACHKIK_004051"
     CDS             483..2411
                     /gene="thrS"
                     /locus_tag="ACHKIK_004051"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO9085123.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2415..2957
                     /gene="infC"
                     /locus_tag="ACHKIK_004052"
     CDS             2415..2957
                     /gene="infC"
                     /locus_tag="ACHKIK_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO9085124.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3054..3251
                     /gene="rpmI"
                     /locus_tag="ACHKIK_004053"
     CDS             3054..3251
                     /gene="rpmI"
                     /locus_tag="ACHKIK_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO9085125.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3304..3660
                     /gene="rplT"
                     /locus_tag="ACHKIK_004054"
     CDS             3304..3660
                     /gene="rplT"
                     /locus_tag="ACHKIK_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO9085126.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3777..3901
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3783..3827
                     /gene="pheM"
                     /locus_tag="ACHKIK_004055"
     CDS             3783..3827
                     /gene="pheM"
                     /locus_tag="ACHKIK_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO9085127.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4111..5094
                     /gene="pheS"
                     /locus_tag="ACHKIK_004056"
     CDS             4111..5094
                     /gene="pheS"
                     /locus_tag="ACHKIK_004056"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO9085128.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5109..7496
                     /gene="pheT"
                     /locus_tag="ACHKIK_004057"
     CDS             5109..7496
                     /gene="pheT"
                     /locus_tag="ACHKIK_004057"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO9085129.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7501..7800
                     /gene="ihfA"
                     /locus_tag="ACHKIK_004058"
     CDS             7501..7800
                     /gene="ihfA"
                     /locus_tag="ACHKIK_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO9085130.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7901..8881
                     /gene="btuC"
                     /locus_tag="ACHKIK_004059"
     CDS             7901..8881
                     /gene="btuC"
                     /locus_tag="ACHKIK_004059"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO9085131.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            8944..9495
                     /gene="btuE"
                     /locus_tag="ACHKIK_004060"
     CDS             8944..9495
                     /gene="btuE"
                     /locus_tag="ACHKIK_004060"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO9085132.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9495..10244
                     /gene="btuD"
                     /locus_tag="ACHKIK_004061"
     CDS             9495..10244
                     /gene="btuD"
                     /locus_tag="ACHKIK_004061"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO9085133.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10322..10786
                     /gene="nlpC"
                     /locus_tag="ACHKIK_004062"
     CDS             10322..10786
                     /gene="nlpC"
                     /locus_tag="ACHKIK_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO9085134.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
BASE COUNT         2720 a         2559 c         2936 g         2645 t
ORIGIN      
        1 tgattaatta ttatcgccag aacgaaatgt gcacgaaaaa ttgttataag aattgtgtca
       61 cttttatcat tgtgatatga ccacttcgtc attaatctca tgagcgttta aaatactacc
      121 gccaaatcac gcaacgaact ttgctggatt cagtttatgc tgctgtaaat ccgctcgcag
      181 taaacctttc agacgcaccg tgatgttatc agtggttctt taagcgtttt gctggtgtac
      241 tcactacaaa cgaattgcga atcgaatcaa tgtgaaacgg aaaggtacaa tctccctttt
      301 tttagtgtta acatcgctca accgggtgga aggtgagagg ttaatgtctt tcatttgtgt
      361 acctgattgc gaaccaattt agcatttgtt ggctagatgg tttcgcaatg aactgttaat
      421 aaacaaattt ttctttgtat gtgatctttc gtgtgggtca ccactgcaaa taaggatata
      481 aaatgcctgt tataactctt cctgatggca gccaacgcca ttacgaccac gctgtaagcc
      541 ccatggatgt tgcgctggac attggtccag gtctggcgaa agcctgtatc gcagggcgcg
      601 ttaatggcga actggttgat gcttgcgatc tgattgaaaa cgacgcacaa ctgtccatca
      661 ttaccgccaa agacgaagac ggtctggaga tcattcgtca ctcctgtgcg cacctgttag
      721 ggcacgcgat taaacaactt tggccgcata ccaaaatggc aatcggcccg gttattgaca
      781 acggttttta ttacgacgtt gatcttgacc gcacgttaac ccaggaagat gtcgaagcac
      841 tcgagaagcg gatgcatgag cttgctgaga aaaactacga cgtcattaag aagaaagtca
      901 gctggcacga agcgcgtgaa actttcgcca accgtgggga gagctacaaa gtctccattc
      961 ttgacgaaaa catcgcccat gatgacaagc caggtctgta cttccatgaa gaatatgtcg
     1021 atatgtgccg cggtccgcac gtaccgaaca tgcgtttctg ccatcatttc aaactaatga
     1081 aaacggcagg ggcttactgg cgtggcgaca gcaacaacaa aatgttgcaa cgtatttacg
     1141 gtacggcgtg ggcagacaaa aaagcgctta acgcttacct gcagcgcctg gaagaagccg
     1201 cgaaacgcga ccaccgtaaa atcggtaaac agctcgacct gtaccatatg caggaagaag
     1261 cgccgggtat ggtattctgg cacaacgacg gctggaccat cttccgtgaa ctagaagtgt
     1321 ttgttcgttc taaactgaaa gagtaccagt atcaggaagt taaaggtccg ttcatgatgg
     1381 accgtgtcct gtgggaaaaa accggtcact gggacaacta caaagatgca atgttcacca
     1441 cgtcttctga gaaccgtgaa tactgcatta agccgatgaa ctgcccgggt cacgtacaaa
     1501 ttttcaacca ggggctgaag tcttatcgcg atctgccgct gcgtatggcc gagtttggta
     1561 gctgccaccg taatgagccg tcaggttcgc tgcatggcct gatgcgcgtg cgtggattta
     1621 cccaggatga cgcgcatatc ttctgtactg aagaacaaat tcgcgatgaa gttaacggat
     1681 gtatccgttt agtctatgat atgtacagca cttttggctt cgagaagatc gtcgtcaaac
     1741 tctccactcg tcctgaaaaa cgtattggca gtgatgaaat gtgggatcgt gctgaggcgg
     1801 acctggcggt tgcgctggaa gaaaacaaca tcccgtttga atatcaactg ggtgaaggcg
     1861 ctttctacgg tccgaaaatt gaatttaccc tgtatgactg cctcgatcgt gcatggcagt
     1921 gcggtacagt acagctggac ttctctttgc cttctcgtct gagcgcttct tatgtaggcg
     1981 aagacaatga acgtaaagta ccggtaatga ttcaccgcgc aattctggga tcgatggaac
     2041 gtttcatcgg tatcctgacc gaagaattcg ctggtttctt cccgacctgg cttgcgccag
     2101 ttcaggttgt tatcatgaat attaccgatt cacagtctga atacgttaac gaattgacgc
     2161 aaaaactatc aaatgcgggc attcgtgtta aagcagactt gagaaatgag aagattggct
     2221 ttaaaatccg cgagcacact ttgcgtcgcg tcccatatat gctggtctgt ggtgataaag
     2281 aggtggaatc aggcaaagtt gccgttcgca cccgccgtgg taaagacctg ggaagcatgg
     2341 acgtaaatga agtgatcgag aagctgcaac aagagattcg cagccgcagt cttaaacaat
     2401 tggaggaata aggtattaaa ggcggaaaac gagttcaaac ggcgcgccct aaccgtatca
     2461 atggcgaaat tcgcgcccag gaagttcgct taacaggtct ggaaggcgag cagcttggta
     2521 ttgtgagtct gagagaagct ctggagaaag cagaagaagc cggagtagac ttagtcgaga
     2581 tcagccctaa cgccgagccg ccggtttgtc gtataatgga ttacggcaaa ttcctctatg
     2641 aaaagagcaa gtcttctaag gaacagaaga aaaagcaaaa agttatccag gttaaggaaa
     2701 ttaaattccg tcctggtaca gatgaaggcg actatcaggt aaaactccgc agcctgattc
     2761 gctttctcga agagggtgat aaagccaaaa tcacgctgcg tttccgcggt cgtgagatgg
     2821 cgcaccagca aatcggtatg gaagtgctta atcgcgtgaa agacgatttg caagaactgg
     2881 cagtggtcga atccttccca acgaagatcg aaggccgtca gatgatcatg gtgctcgctc
     2941 ctaagaagaa acagtaaggc cttcaagtag cactgtctgt ggagccttcg ggcttcacag
     3001 gctttgttcg cctttgtttc gtttattaac aatgcgaagt ggaagttatt aaaatgccaa
     3061 aaattaagac cgtacgcggt gctgctaagc gcttcaaaaa aaccggtaaa ggtggtttta
     3121 agcacaagca cgctaacctg cgtcacattc tgaccaaaaa agcgaccaaa cgtaaacgtc
     3181 acctgcgtcc gaaagccatg gtttccaaag gcgatctggg cctggtaatc gcgtgcctgc
     3241 cgtacgcata agccgttaac gtttttaact ttttaattag aatatagata caggagagca
     3301 catatggctc gcgtaaaacg tggtgttatt gcacgcgcac gtcacaagaa aattttgaaa
     3361 caagctaaag gctactacgg tgcgcgttct cgcgtatacc gcgttgcctt ccaggctgtt
     3421 atcaaagctg gtcagtatgc ttaccgtgac cgtcgtcaac gtaagcgtca gttccgtcaa
     3481 ctgtggattg cgcgtatcaa cgcagcagca cgtcagaacg gtatttctta cagcaaattc
     3541 atcaatggcc tgaaaaaagc ctctgttgaa atcgaccgta agatcctggc tgatatcgca
     3601 gtattcgaca aagtagcgtt caccgctctg gttgaaaaag cgaaagcagc tctggcataa
     3661 gccagttgaa agagggagct agtctccctc ttttcgtttc aacgccatca aaacattgac
     3721 ttttatcgcc gtagcctttt caataaaggt cttttgaaga gtaccaaaag gtaacgcaag
     3781 caatgaatgc tgctattttc cgcttctttt tttactttag cacctgaatc caggaggcta
     3841 gcgcgtgaga agagaaacgg aaaacagcgc ctgaaagcct cccagtggag gctttttttg
     3901 tatgcgcgtt ttgaaaaata aaaagcctgt ctcatcaggt gtaattgtcg cagccagtct
     3961 ggacacggcc agcgcggaga aaccggagcg tatttaagta cgtgagaatt tcgagcactg
     4021 cccgggacca aaatggcaag taaaatagcc tgatgggata ggctctaagt ccaacgaacc
     4081 agtgtcacca ctgacacaat gaggaaaacc atgtcacatc tcgcagaact ggttgccagt
     4141 gcgaaggcgg ccattagcca ggcgtcagat gttgccgcgt tagataatgt gcgcgtcgaa
     4201 tatttgggta aaaaagggca cttaaccctt cagatgacga ccctgcgtga gctgccgcca
     4261 gaagagcgtc cggcagctgg tgcggttatc aacgaagcga aagagcaggt tcagcaggcg
     4321 ctgaatgcgc gtaaagcgga actggaaagc gctgcactga atgcgcgtct ggcggcggaa
     4381 acgattgatg tctctctgcc aggtcgtcgc attgaaaacg gcggtctgca tccggttacc
     4441 cgtactatcg accgtatcga aagtttcttc ggtgagcttg gctttaccgt ggcaaccggg
     4501 ccggaaatcg aagacgatta tcataacttc gatgctctga acattcctgg tcaccacccg
     4561 gcgcgcgctg accacgacac tttctggttt gacactaccc gcctgctgcg tacccagacc
     4621 tctggcgtac agatccgcac catgaaagcc cagcagccac cgattcgtat cattgcgcct
     4681 ggccgcgttt atcgtaacga ctacgaccag actcacacgc cgatgttcca tcagatggaa
     4741 ggtctgattg ttgataccaa catcagcttt accaacctga aaggcactct gcacgatttc
     4801 ctgcgtaact tctttgagga agatttgcag attcgcttcc gtccttccta cttcccgttc
     4861 accgagcctt ctgcagaagt tgacgtcatg ggtaaaaacg gtaaatggct ggaagtgctg
     4921 ggttgcggga tggtgcatcc gaacgtgctg cgtaacgttg gcatcgaccc ggaagtttac
     4981 tctggtttcg ccttcgggat ggggatggag cgtctgacta tgttgcgtta cggcgtcacc
     5041 gacctgcgtt cattcttcga aaatgatctg cgtttcctca aacagtttaa ataaggcagg
     5101 aatagattat gaaattcagt gaactgtggt tacgcgaatg ggtgaacccg gcgattgata
     5161 gcgatgcgct ggcgaatcaa atcactatgg cgggcctgga agttgacggt gtagaaccgg
     5221 ttgccggtag cttccacggc gtggtcgttg gtgaagtggt tgagtgtgcg cagcatccga
     5281 acgctgacaa actgcgtgtg acaaaagtga atgttggcgg cgatcgcctg ctggacatcg
     5341 tctgcggtgc gccaaactgc cgtcagggcc tgcgtgtggc ggtagctacc attggtgctg
     5401 ttctgccggg tgatttcaaa attaaagcgg cgaaactgcg tggtgaaccg tctgaaggga
     5461 tgctgtgctc cttttctgag ctgggaattt ctgacgatca tagcggcatt atcgagctgc
     5521 ctgcggatgc gccgattggc accgacatcc gtgaatacct gaaacttgat gacaacacca
     5581 tcgaaatcag cgtgacgcca aaccgtgccg actgtttagg tatcattggt gttgcgcgtg
     5641 acgttgccgt gctgaatcag ctgccgctgg ttgaaccgga aatcgttccg gttggtgcga
     5701 ccatcgacga cacgctgccg attacagtcg aagcgccgga agcctgcccg cgttatcttg
     5761 gccgtgtggt aaaaggcatt aacgttaaag cgccaactcc gctgtggatg aaagaaaaac
     5821 tgcgtcgttg cgggatccgt tctatcgatg cagttgttga cgtcaccaac tatgtactgc
     5881 tcgaactggg ccagccgatg cacgctttcg ataaagatcg cattgaaggc ggcattgtgg
     5941 tgcggatggc gaaagagggc gaaacgttgg tactgcttga tggcactgaa gcgaagctga
     6001 atgctgacac tctggtcatc gccgaccaca acaaggcgct ggcgatgggc ggcatctttg
     6061 gtggcgaaca ctctggcgtg aatgacgaaa cgcaaaacgt cttgctggaa tgcgctttct
     6121 tcagcccgct gtctatcacc ggtcgtgctc gtcgtcatgg cctgcatact gatgcgtctc
     6181 accgttatga gcgtggcgtt gatccggcac tgcagcacaa agcgatggaa cgtgcgaccc
     6241 gtctgctgat cgacatctgc ggtggtgagg ctggtccggt aattgatatc accaacgaag
     6301 caacgctgcc gaagcgtgca accatcactt tacgtcgtag caaactggat cgcctgatcg
     6361 gccatcatat tgcggatgag caggtaactg acattctgcg tcgtctcggc tgcgaagtga
     6421 ccgaaggcaa agacgagtgg caggcagttg cgccgagctg gcgtttcgat atggagattg
     6481 aagaagatct ggtcgaagaa gtcgcgcgtg tttacggcta caacaacatc ccggatgagc
     6541 cggtacaggc aagcctgatt atgggtactc accgtgaagc tgacctgtcg ctcaagcgcg
     6601 tgaaaacgct gctcaacgac aaaggctatc aggaagtgat cacctacagc ttcgttgatc
     6661 cgaaagtgca gcagatgatc catccaggcg ttgaagcctt actgctgcca agcccgatct
     6721 ctgttgaaat gtcagcaatg cgtctttctc tgtggaccgg cctgctggca accgtggtgt
     6781 acaaccagaa ccgtcagcag aaccgtgtgc gcattttcga aagcggtctg cgtttcgtac
     6841 cagatactca ggcaccgttg ggcattcgtc aggatctgat gttagccggt gtgatttgcg
     6901 gtaaccgtta cgaagagcac tggaacctgg caaaagagac cgttgatttc tatgatttga
     6961 aaggcgatct tgaatccgtt ctcgacctga ccggtaaact gaatgaggtt gagttccgtg
     7021 cagaagcgaa tccggcactg catccggggc aatccgcagc gatttatctg aaaggtgaac
     7081 gtattggttt tgttggggtt gttcatcctg aactggaacg taaactggat cttaacggtc
     7141 gcactctggt gttcgaactg gagtggaaca agctcgcaga ccgcgtggtg cctcaggcgc
     7201 gcgagatttc tcgcttcccg gcgaaccgtc gtgacatcgc ggtggtggtc gcagaaaacg
     7261 ttcccgcagc ggatatttta tccgaatgta agaaagttgg cgtaaatcag gtagttggcg
     7321 taaacttatt tgacgtgtac cgcggtaagg gtgttgcgga ggggtataag agcctcgcca
     7381 taagcctgat cctgcaagat accagccgta cactcgaaga agaggagatt gccgctaccg
     7441 tcgccaaatg tgtagaggca ttaaaagagc gattccaggc atcattgagg gattgaacct
     7501 atggcgctta caaaagctga aatgtcagaa tatctgtttg ataagcttgg gcttagcaag
     7561 cgggatgcca aagaactggt tgaactgttt ttcgaagaga tccgtcgcgc tctggaaaac
     7621 ggcgaacagg tgaaactctc tggttttggt aacttcgatc tgcgtgataa gaatcaacgc
     7681 ccgggacgta acccgaaaac gggcgaggat attcccatta cagcacggcg cgtggtgacc
     7741 ttcagacccg ggcagaagtt aaaaagccgg gtcgaaaacg cttcgcccaa agacgagtaa
     7801 tctgatctaa ctaaaaaggc cgctctgcgg ccttttttct tttcactgtc gaagagtcac
     7861 cgtaaaatca acgccatgac acttcagcag aacggatacc atgctgacac ttgcccgcca
     7921 acaacagcga caaaatattc gctggttatt atgcctgtca gttttgatgc tgctggcgct
     7981 tctcttaagc ctttgcgccg gtgaacaatg gatctcgcca ggtgactggt ttactcctcg
     8041 tggcgaactg ttcgtctggc aaattcgcct gccacgtacg ctggctgtat tgctggttgg
     8101 tgcggcgctg gctatatccg gcgctgtaat gcaggcgttg tttgaaaatc ctctggcaga
     8161 acctggacta cttggcgtct ctaacggcgc aggcgtgggg cttatcgccg cggtattgct
     8221 tgggcaaggg caactcccca actgggcgct agggctgtgt gcgattgctg gcgcgcttat
     8281 catcacttta atactcttac gtttcgcccg tcgtcatctt tcgaccagtc ggttattgct
     8341 ggctggcgtt gcattaggga ttatctgtag cgcactaatg acgtgggcta tctacttttc
     8401 cacctctgtt gatttacgtc agctgatgta ctggatgatg ggcggttttg gcggcgtaga
     8461 ctggcggcaa agctggctga tgctggcatt gatccccgtg ttgttgtgga tctgttgtca
     8521 gtccaggccg atgaatatgt tagcacttgg cgagatctcg gcgcggcaac tgggtttacc
     8581 cctgtggttc tggcgcaatg tgctggtggc cgcgaccggc tggatggttg gcgtcagtgt
     8641 ggcgctggcg ggtgctatcg gctttattgg tctggtgatc ccacatattc ttcggttgtg
     8701 tggtttaacc gatcatcgcg tattacttcc cggctgcgcg ctggcagggg cgagcgcatt
     8761 gctgctggcc gatattgtag cgcgcctggc attagctgcc gcagagctgc ctattggcgt
     8821 ggtcaccgca acgttgggtg cgccggtgtt tatctggtta ttgttaaaag caagacgtta
     8881 gccgcaaaaa gacggtctat gattaaaagc taagattttt actgaccaca cccaggagaa
     8941 acgatgcaag attccattct gacgaccgta gtgaaagata tcgacggtga agtgaccacg
     9001 ctggagaagt acgctggtaa tgtgctgttg attgtcaatg tcgcctcaaa gtgtggctta
     9061 acgccgcaat atgagcagtt ggagaatatt cagaaagcct gggccgatcg aggttttgtg
     9121 gtgctgggat tcccgtgcaa ccagtttctg gaacaagaac cgggcagcga tgaagagatt
     9181 aaaacttact gtaccaccac atggggggtg acgttcccga tgttcagtaa gattgaagtt
     9241 aatggcgaag gacgccatcc gctgtatcaa aaattgattg ccgcagcgcc gaccgcagtc
     9301 gcgccggaag agagcggatt ctatgcccgt atggtcagca aaggtcgtgc accgctgtac
     9361 cctgatgata ttttatggaa ttttgagaaa tttttagttg gcagggacgg gttggtcatc
     9421 cagcgttttt ccccggatat gacgcctgaa gatcccattg tgatggaaag cattaaactg
     9481 gcgttggcaa aataatgtct attgtgatgc agttacagga tgttgcggaa actacccgcc
     9541 tggggccgct ttctggcgag gttcgggctg gggagatcct gcacctggtg gggccgaatg
     9601 gcgcgggtaa gagtaccttg cttgcacgaa tggccggaat gaccagcggt aagggaagca
     9661 ttcagttcgc ggggcaacca ctggaagcat ggtccgcaac aaaactcgcg ctgcatcgcg
     9721 cctacctttc acaacagcag acgccgccgt ttgcaatgcc ggtctggcac tacctgacac
     9781 tgcatcagca cgataaaacg cgtaccgaac tactgaatga tgtcgcaggg gcgctggctc
     9841 ttgatgacaa actcggacgt agcaccaatc aactttccgg cggtgaatgg caacgcgtac
     9901 gtcttgctgc ggtggtgctg caaatcacac cacaagccaa tcccgcaggt caattgctgc
     9961 ttcttgatga gccgatgaac agtcttgatg ttgcgcaaca aagtgcgtta gacaaaattc
    10021 tgagcgctct gtgtcagcaa ggactggcga ttgtgatgag cagtcacgat ctcaaccaca
    10081 cattgcgtca tgcgcatcgg gcgtggttgc taaaaggtgg aaaaatgctg gccagtggac
    10141 gcagagaaga ggtgctcacg ccgccaaatc tggcgcaggc ctatgggatg aattttcgcc
    10201 gtctggatat cgaaggtcac agaatgctga tttcgaccat ctgatttcat tagtatcttg
    10261 aaaatcgggt aattacgacg ctaaattaat gcaagaataa aaaacagagg aaccgtacgg
    10321 aatgcgtttc tgccttattt tgatcacagc actgtttctg gccgggtgta gccaccataa
    10381 agcaccgccg ccaaatgcca gactttctga ttcgattacc gttattgccg gtttgaacga
    10441 ccagctacaa agctggcatg gcacgccgta tcgttatggt ggcatgacgc ggcgcggtgt
    10501 ggactgttcg ggatttgtgg ttgtaacgat gcgcgatcgt ttcgatttgc agctgccccg
    10561 agaaaccaaa gaacaagcct ctatcggcac gcaaattgat aaagacgagt tgctgcctgg
    10621 tgacctggtc tttttcaaaa cgggttccgg acaaaatggt ttgcatgtag gtatttacga
    10681 taccaacaac caatttatcc acgcctctac cagcaaggga gtgatgcgtt cctcacttga
    10741 taatgtctat tggcagaaaa atttctggca ggcgagacga atttagtatg tcgaaaaatg
    10801 gacggcgatg atacatcgcc gtccatttac tcctgaatac agtagtattt tgcagacaaa
//
LOCUS       DAZGJW010000150        10781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZGJW010000150 DAZGJW010000000
VERSION     DAZGJW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.150"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            118..831
                     /gene="yghS"
                     /locus_tag="ACHKIK_004063"
     CDS             118..831
                     /gene="yghS"
                     /locus_tag="ACHKIK_004063"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO9085135.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            863..1621
                     /locus_tag="ACHKIK_004064"
     CDS             863..1621
                     /locus_tag="ACHKIK_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085136.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1667..3016
                     /locus_tag="ACHKIK_004065"
     CDS             1667..3016
                     /locus_tag="ACHKIK_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO9085137.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            3016..3840
                     /locus_tag="ACHKIK_004066"
     CDS             3016..3840
                     /locus_tag="ACHKIK_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085138.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            3852..4412
                     /locus_tag="ACHKIK_004067"
     CDS             3852..4412
                     /locus_tag="ACHKIK_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO9085139.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFIIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4443..5513
                     /gene="lptF"
                     /locus_tag="ACHKIK_004068"
     CDS             4443..5513
                     /gene="lptF"
                     /locus_tag="ACHKIK_004068"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO9085140.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            5510..6589
                     /gene="lptG"
                     /locus_tag="ACHKIK_004069"
     CDS             5510..6589
                     /gene="lptG"
                     /locus_tag="ACHKIK_004069"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO9085141.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     VLVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(6625..7797)
                     /locus_tag="ACHKIK_004070"
     CDS             complement(6625..7797)
                     /locus_tag="ACHKIK_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO9085142.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYSSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQINAIIQAFATLKQ"
     gene            complement(7797..8045)
                     /locus_tag="ACHKIK_004071"
     CDS             complement(7797..8045)
                     /locus_tag="ACHKIK_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO9085143.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8077..8991)
                     /locus_tag="ACHKIK_004072"
     CDS             complement(8077..8991)
                     /locus_tag="ACHKIK_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO9085144.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDSVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(8988..10676)
                     /locus_tag="ACHKIK_004073"
     CDS             complement(8988..10676)
                     /locus_tag="ACHKIK_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO9085145.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
BASE COUNT         2600 a         2679 c         2821 g         2681 t
ORIGIN      
        1 ttatgaaagc gtaattcagg tcaatgtcac aattaaccat tgtcacaata aggttgaacg
       61 gatattctga gcacggacca cgtaatattc aatacattat ttactgccgg gtttttcgtg
      121 aatcaacgta atatgtcaat aattaattcc acgccagtgc gtgttattgc cattgtagga
      181 tgtgatggtt caggtaaatc gaccctcacg gcaagcctgg taaatgaact ggcagcaaga
      241 atgccaacag aacgcattta tctcgggcaa tcgtccgggc gaattggcga atggatttca
      301 cagctccctg ttattggcgc accttttggg cgttatctgc gaagtaaagc ggcacatgtg
      361 cacgaaaagc cctcaacacc gcctggcaat attactgcgc tggttatcta tctgctttcc
      421 tgctggcggg cgtacaagtt tcgcaaaatg ttgtgtaaaa gccagcaagg ctttctgctc
      481 atcaccgacc gctacccgca ggttgaagtg ccgggttttc gctttgatgg cccgcaattg
      541 gcaaaaacca cgggcggtaa cggttggata aaaatgttaa ggcagcgcga gctgaagctg
      601 taccaatgga tggcatctta tttgcccgta ttgttgattc gtcttggcat tgatgaacaa
      661 accgcgtttg cgcgtaaacc tgaccaccaa ctggcagcgt tacaggaaaa aatcgccgtt
      721 acgccgcaac tgacattcaa tggcgcaaag atccttgaac tggatgggcg acaccccgcc
      781 gatgaaattc tgcaagcgtc actacgcgca attcacgccg cgctttcctg atcaccaaaa
      841 tggatagggg atgatttaac gaatggatgc actacaaact caaactgtta atagtacaac
      901 cgcgccgcag cccaactaca ttccggggct gattgcggtg gtcgggtgtg atggcaccgg
      961 taaatccaca ctgaccaccg acctggtgaa atcgctgcaa caacactggc aaaccgagcg
     1021 gcgctatctg gggctgctct ccggcgaaga cggcgacaaa atcaaacgat tgccgttggt
     1081 tggcgtctgg ctggaacggc gactggcggc caaatcctcg aaaacccaaa gcatgaaaac
     1141 caaatctccg gcgctatggg cggcggtgat tatgtactgc ttctcgctgc gaagaatggc
     1201 gaatctacgc aaggttcagc gactggcgca aagtggcgtt ctggtggtca gcgatcgctt
     1261 cccgcaggct gaaatttcgg gcttttatta tgatggaccg gggattggcg tcgaacgtgc
     1321 gaccgggaaa atcagcatgt ttctggcgca gcgcgaacgg cgtttatacc aacaaatggc
     1381 gcaatatcgc ccggaattaa ttattcgcct gggcattgat attgagactg ccatctcccg
     1441 caagcctgac catgactatg ccgagctgca ggacaaaatc ggcgtcatgt cgacgattgg
     1501 ctataacggc acaaaaattc ttgagattga ttccagagcg ccctacagcg aagtgctgga
     1561 acaggcacag aaggcggttt ctctggttgc catcgtttct gaccgccgaa gtttaactta
     1621 aggctggcgg gatacttttt actatcaacg ataaggtcag gctgaattgg cgggttttaa
     1681 catcaaacat tggtttgcag atggcgcgtt tcgcaccatt attcgcaata gcgcctggtt
     1741 aggctccagt aatgtcgtga gcgccttgct gggtctgttg gcgctctcgt gtgccggtaa
     1801 agggatgacg cccgccatgt ttggcgtact ggtgattgtg caatcgtacg ccaagtcgat
     1861 cagcgatttt attaagtttc agacatggca actggtggtt cagtacggaa cgccagcctt
     1921 gaccaacaat aatccgcagc aatttcgcaa tgtcgtctca ttttccttct cgctggatat
     1981 cgtcagcggc gcggtggcga ttgtcggcgg cattgcctta ctgccattcc tttcccattc
     2041 attaggtctg gatgaccaaa gcttttggct ggcggcgctg tattgcacgc tcattccttc
     2101 aatggcttcc tccacgccga ccggcattct gcgtgcggta gatcgcttcg atttaattgc
     2161 tgtacagcag gcgacgaaac cttttctgcg cgcagcgggg agcgtcgtag cctggtattt
     2221 tgactttggt tttgcgggtt ttgttattgc ctggtacgtg tcgaatctgg ttggcggcac
     2281 catgtactgg tggtttgcag cgcgcgaatt acgccgcaga aatatccata atgccttcaa
     2341 attgaatctg tttgagtctg cccgacacat caaaggcgcg tggagttttg tctggtcaac
     2401 caacattgcc cactccatct ggtcggcgcg taactcgtgc agcacagtgc tggtggggat
     2461 cgtgttagga cccgctgccg ccgggttatt taaaatcgcc atgacattct tcgacgccgc
     2521 cggaacgcca gcgggtttgc tgggtaaaag tttttaccca gaggtgatgc gtttagatcc
     2581 gcgcaccacc agaccgtggt tgttaggtgt gaagtccggt ttactggcgg gcggaatcgg
     2641 tattctggtg gcgctcgcgg tgtttatcgt cggcaagccg ctcatttcgc tggtgtttgg
     2701 cgttaagtat ctcgaagcgt atgacctgat tcaggtgatg ctgggcgcaa ttgtgatttc
     2761 gatgctgggc ttccctcagg agtcattgct attgatggcg ggaaaacagc gcgcttttct
     2821 cgtggcgcaa accatcgcct caatcggcta catcgtattg ctgttcatgt tctgtcatct
     2881 gtttggtgtg ctgggagcag cgtttgctta cttcggtggg caatgtctgg acgtggcgct
     2941 ctcgttaatt ccaaccttaa aggcattttt tcagcgccat tccttgcttt ataacgcagc
     3001 cggagagaaa tcctgatgat gagaaaatat ttcccgcttg aggcgagcga gcgtctattt
     3061 gttgcgattg aagaagatga tgtcgttgat gcacaggtta gtttgccgcc gactattgca
     3121 ctaagttgta ccaccgaaat cattcatgat aattacgcgt tatgcctgca attctggctc
     3181 aatggcgttg atcgtcagga attactgcgt ttagttcgca aacaagccaa aggcgatgag
     3241 ctgactgctg atgagcgcaa acaatttaaa tacatgcgag cgcgctacaa gcacctgcgt
     3301 tttgcgcaac ggctgtattt aaagaagcat caggccggat ttttattcgg caaaacgacc
     3361 gtttttctgg ggcgttttca ggacggcttt cgtaacggta agaaaaatat tgtgtcgtat
     3421 tacggcaacc tgttacggat ttatttaagc tctccggtct ggtcgttggt gaactattca
     3481 ttacgccata gccagttaga aagcgtaagc agttttattg cctatcggca aaagcaaatg
     3541 cataccctga aagagattat tgccaaaccg cgactaaccg gcagggaatt tcatgatgtg
     3601 cgaaaaatca tcagccaaca ggtttcttat tacgacacct taagatcgct tgatcctgaa
     3661 aataaagagg cgttacagat ttcccgtttc ctcgccgcca ttaatggcct gatgggagat
     3721 aaacacgacg acatggtggc ggatgatatg gaaaaccgcc agtcgtatga tgcaccgttg
     3781 gcgctggata gcgatattcg tcagcgctta gagctgctga tttcgcgttt tccgctatag
     3841 ccggagcaat gatgtcatct cgtttgatta ttgcgttgat tatcatgctc ttagccccgg
     3901 gtgtgcaggc acacaatttc gtcactggca aaacggttac ccctgtttat atccaggaag
     3961 gcggcgagct gcttctgaat agcgacgacg aaattcacta ccaaaagtgg aacagcacac
     4021 aattagcagg aaaagtccgc attattcagt atatcgccgg gcgtaagtcg gcgaagaaaa
     4081 agaactcact gttaattaaa gcggttgaag cggcaaactt tccgcaggat cgttttcagc
     4141 ccaccaccat tgtgaacacc gatgatgcca tctttggtac tggctatttt gttgttggca
     4201 aaatcgaaaa aaataaacgc cgctatccgt gggcgcaatt cattattgac ggcaatggac
     4261 agggacgcgt tgcctggcgc ttgccggagc agagttcaac cattctggtg ctgaataaag
     4321 ccgggcaaat tcagtgggca aaggacggca gcctgacgcc ggaagaggta gatcatgtca
     4381 ttgcgctggc gcagaaactg atcaatgaat aaaaaattca aaggattgag ggcgttagtg
     4441 agatgaagct ggttgaacac tacatcatgc gtggtacgcg ccgtctggtg ctgattatcg
     4501 tcggttttct gatctttatt ttcgccagct actccgcaca gcgttatctg accgaagcgg
     4561 caaatgggac attagctctt gatgttgtgc tggatatcgt tttctacaaa gtgctgattg
     4621 cactggagat gttgttacct gttggtctgt atgtgtcagt tggcgtgacg ctagggcaga
     4681 tgtataccga ctcggaaatt accgctatct ctgcggcggg gggcagtccg ggacgcttgt
     4741 acaaagccgt tctttatctg gcgataccgc taagtatttt tgtcaccctt ctgtcgatgt
     4801 atggtcgtcc gtgggcttat acgcagattt atcaactgga gcagcagtcg cagtcggagc
     4861 tggatgttcg ccagttgcgg gcaaagaaat ttaacactaa cgataacgga cgaatgatcc
     4921 tttcgcagac ggttgatcag gataataatc gcctgactga cgcgctgatt tatacttcta
     4981 ctgccaatcg aacccgcatt ttccgcgccc gttcggttga tgtggttgac ccatcacccg
     5041 agaaaccgac cgttatgttg cacaacggca ctgcctatct tctcgatcat caggggcgtg
     5101 acgacaacga acagatctac cgtaatctgc aattacatct gaatccgctg gatcaaagcc
     5161 ctaacgtcaa acgcaaagca aaatcggtca cggagctggc gcgctccgcc tttcctgccg
     5221 atcatgccga actgcaatgg cgacaaagcc gtggcctgac agcattgttg atggcgctgc
     5281 tggccatttc attaagtcgg gtaaaaccgc ggcaagggcg attttcaaca ttattgccac
     5341 tgacgttgct gtttattgcc attttttatg gcggcgacgt ctgccgtacg ctggtggcta
     5401 acggcgcgat tcccctcatt cctggtttgt ggttagtacc cggactcatg ctaatgggcc
     5461 tgctgatgct ggtcgcacgc gacttctctt tgctgcagaa attttcccga tgaatgtttt
     5521 cagtcgctat ttaatccgtc atctctttct cggttttgcc gccgccgcag ggctattgct
     5581 gccgcttttt accaccttca acctgattaa cgaactggat gatgtcagcc cgggcggtta
     5641 tcgctggact caggcggtgc tggtggtgct aatgacctta ccgcgcacac tggtcgaact
     5701 ttcgccgttt atcgcgttat tgggtggtat tgttggactg gggcagttat cgaaaaacag
     5761 tgagcttacc gccattcgca gcacggggtt ttctatcttc cgtattgcac tggtggtgct
     5821 ggttgccggg atattgtgga ctgtttcgtt aggcgcgata gatgagtggg tggcgtcgcc
     5881 attgcagcaa caggcgttgc aaatcaaatc gaccgccacc gcgttggggg aggacgatga
     5941 cattaccggc aatatgctgt gggccaggcg tggtaatgaa tttgtgacgg tgaaatcgct
     6001 gaacgagcag ggccagccgg tgggcgtgga gatatttcat tatcgcgacg atctctcgct
     6061 tgaatcctac atttatgcac gcagtgccac cattaaagac gacaaaacgt gggtcctgca
     6121 tggtgtgaat cataaaaaat ggctgaatgg caaagaaacg ctggaaacat tagataatct
     6181 tgcctggcaa tcggccttca ccagtatgaa tcttgaagag ttatcaatgc cggggaatac
     6241 tttttctgtc cgtcagctta atcattacat ccattatttg caggaaaccg ggcaacccag
     6301 cagcgaatac cgccttgcac tgtgggaaaa actggggcaa cccatcctga ccctggcgat
     6361 gattttgctg gctgtgccgt ttacctttac tgccccgcgt tcgcctggga tgggtagccg
     6421 tctcgctgta ggcgtcatcg tgggcttact cacctggatc agctatcaaa tcatggttaa
     6481 tctgggattg ctatttgcgt taagcgcacc tgttaccgcg ctcggtttac cggtagcgtt
     6541 tgtgttggtg gcgttgagcc tggtgtattg gtatgacaga caacattaaa cccgccgtgg
     6601 cgcaaacacc acggcgggct cacattactg cttcagagta gcaaatgcct gaatgatggc
     6661 attgatttgc tcacgactgt gggcagcatt tacgctacag cgcagcagcg tgatgcctgc
     6721 aggcgcggca ggcggcagga taagattcac atacacgccc agcgagataa gctcgcgcca
     6781 gatgcgtaaa ccttcctctt tcgaaccgat tattactggc actaccgggc taatatgtga
     6841 acttaactca taacccagtt tttgcagacc gtcatacaga tggttggcgt tatccatcag
     6901 cttttgccgc agctctggtt gtgtggcaat agttcttaac gacgagcgca cggtcgcaat
     6961 gcaggacgga gaaggagagg cggtaaaaat atacgggcgg ctgctgtagc gcagcacttc
     7021 catcgcttcc gaaccgacag caaaaccgcc aatcgaggcc agacttttac tgaaagtacc
     7081 aacgataata tccacatcat cttcaacgcc gacagcttca gccagtccgc gccccgttgc
     7141 gcctaacacg ccaaacgaat gcgcttcatc gacaatcaaa taaccaccga gacgacgttt
     7201 gatatcaaca atttccgcca gcggcgcaac gtcaccgagc atgctgtaga tgccctcaac
     7261 gatgataatc gcctctttcg cccgttcacc gaggcgaacc atgcgccgtt ccagatcttt
     7321 agcatcgtta tggcggaaac gaataatttc cgcgccaccg agagaacagg cgtcataaat
     7381 actggcgtgg ctgtcggcat ccagtaacac cacggcgtta tggtcggcaa gtgcgctgat
     7441 gacgcctaaa ttagcggtat aaccggttga aaaaacgata gcggtagggc gattgaaaaa
     7501 ctcggcaatc tctttctcta acgccaggtg cggcgcataa ctgccgtttg ccatacgaga
     7561 accggtggtg ccggtgcctt gggccgctaa cgctgcctga ccttcgctga tggcatcatg
     7621 attaaaggtt aaaccgagat aattattggt cccggccaga atcacttttt tattgccgat
     7681 ccggccttcg gtggcggaat acacttcatc aatacaggtg ccaaacggtg ttaacccgtt
     7741 gtcactaaat tgcagtcgct cgccagcaag gcgtgcatat ttatcgtata gccccattat
     7801 ttattctcca gccatggaag tagagtttcc attaattgcg caggggtttt gacatccagc
     7861 agaatattaa taggaatgga aatatcgaat ctgtcttcca gcatcattaa gagatccatc
     7921 actttgatcg actccaggcc aagatcgttg acgagatcgc tgtcgggctt aatttccaca
     7981 ccgttttcga ccaaatcctg taaacaggaa aggatgtaat ccattactat ttcacgattt
     8041 accatagaaa cgttaacaca cctttaaaca agacaatcaa aatagcccgt tgcgtaatgc
     8101 gtgttccagg ctaatcccgg gaaaccaatt aatatcttca gaaatacggt tattacttgc
     8161 cgaccagtcg gcgtgggtta attcacgaat tttcgagcgg gtcagcatag gttctttacc
     8221 tgccaggcga ctcaacgcgg tactgatatc cgcaaggcag gtgagtaccg gcagaggaat
     8281 accaaccatt cgcacggaac cgcaacggac gttggcggca agttgctgta tgcgttgcca
     8341 gtcatagccg ccagcgacgc tatcgcataa ttcataggtt tgcgtctgta cagtttcggc
     8401 gcttaaccac tgacccactg cttgcgcaaa atcggtgacg tgcaggaaag agagctgtgt
     8461 atctggtgca ccaagtcgtg gcagcaggcc gcgcagcatc cagtcaaaca gcggttttaa
     8521 ctctttatcg ccgggaccat acacggctgt cgggcgaaaa acgcccagcg taatttcgtc
     8581 agccattgca gtcagccgtt gttcggcgat gtgtttggaa tttgcgtacc aggagagctc
     8641 gggatggcgc gccgccagcg aagagataaa cagaaaacgt tggcaaaagc agctctcttt
     8701 tgctgcttgc atcaggcgca ggctgccgtc aacgttacag cgggtgaaaa tctcttcttt
     8761 gtgcccgcgc acttgcccgg cgcaatggac cactgcgctg gctccggcaa ccagcttgct
     8821 aagtgaatga gtatcttcca gcgaaccgcg cacccaggtg agattatcat tgacgtgagc
     8881 gcgggcagta cgcgtcaatg cgcgaacgtg aaagccgcgg gcgagcaggt tatcgataat
     8941 atatttaccg ataaacccgg tagcgcccgt caccgcgaca gtttgattca tgccagggat
     9001 tcctgcacat taagactggc agcataagcc ttctgataac gttttttcgc ttccgcacgg
     9061 gcaggcttgc cggaggacgt tcggggaata ctgtggggcg gcaacagatc gatagccgcg
     9121 gtcacgccaa attcgctttg gatccgtgcc gccagcgcgt ggataagctg cccgcgacgt
     9181 tcttcgtcgc tgatccgaca ctggatctgc aaaatgattt tttcctgggc ggtaacaaaa
     9241 gcaatcgcat cgccagaatg aatttccggt tcttgttccg caatatattc aatatcctgc
     9301 ggccagatat tacggccacg aataataatc agatctttaa tgcgtccggt gacatacaga
     9361 taaccgtcca gcagataacc gaggtcgccg gtgtctaacc agcccgtcgc ggcaatctcg
     9421 tcttgcgaaa cctggtcgcc aaagtaaccg ctcatcaggc tggggccgga gatgcaaata
     9481 tggcctacca cacgttccgc gaccggcata cctgcttcat tgcggatttc aatgccatgt
     9541 tccggcaacg ctttgccgca gttgacgaaa gtcgatacgg cgcgtgtctc tgcacccggc
     9601 gcgacggctt taccctgata ttcgaggatg tcgcgatcca cttcgttaac cacaaccccg
     9661 gaggcttcat cagagaagct gacagccagc gcattttctg ccagtccgta gcacggcatg
     9721 aaagttttat tgtcaaagtt aacctgacgg aaacattcag cgaattgatg gagttgttct
     9781 gcggagatgg gttctgcacc aataccagcg acgcgccagc aggaaagatc cagttcagcg
     9841 agatcttttt cattcacgcg gcgctggcac aattcatagc caaacggcgg cgcaacggaa
     9901 acggtgccgc gatttttact gatcaattta agccattgca gaggacgcat ggcaaaatcc
     9961 tgagtgcgca aataatctac tgaaagctgc gtggcgacgg ggggcaggag aaagccgacc
    10021 agtcccatat catggtagaa aggcagccag gagacgcagc ggtcgccagg gcgtaattta
    10081 atgccgtcgt ggcttatagc acgtagatta gccatcactt cgcgatgggt gataatgacg
    10141 ccacggggaa aacgggtgct gccggaggtg tactggaggt aggcgatatc gttcggaact
    10201 ggacgctgga gcgcaacatc ggcttccggt aatgccttaa accaggcgtg gcttaaaaca
    10261 tgtaattcgg ggttgttatg cgtcgcggca ttgaccagtg gcaaccactc atcaccagtg
    10321 ataatggctg cgggctggca acttgccagt aaaccctgga gtttggcgct ccaggaatcc
    10381 cgctgaccga cgcccattgg aatcgccaac gggacggcga ctaagccagc atactgacag
    10441 gcaaaaaaag cctctacaaa cccgctactc gtttcggcaa tcagcgccac gcgttcgcct
    10501 tttttcaggt tcagcgataa caaccgcttc gcaccagctt cggcacgcgt ttttaatgtc
    10561 tgatattcca gttgatcttc aagttggcaa cgtctgtcat aaaaattcat tccggcgcta
    10621 ctcagagcgg cgtagtccaa agcatcaacc agcgttggaa aatcggcata gcgcataggt
    10681 agggaatgcg taaagatttt attagacata tacaccacga aaaaattgga tatcaaaaat
    10741 aaaatgacaa taatgcggtg gattatatag gcataaaaga t
//
LOCUS       DAZGJW010000151        10773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZGJW010000151 DAZGJW010000000
VERSION     DAZGJW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.151"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            66..299
                     /locus_tag="ACHKIK_004074"
     CDS             66..299
                     /locus_tag="ACHKIK_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9085146.1"
                     /translation="MLRKLIPQNAIARYIGVTPQAVNLWFSKNSVPSRFVLRVCELVE
                     WKVTPHGLRPDLYPYPEDGIPDSLRKSNGITRD"
     gene            381..1088
                     /locus_tag="ACHKIK_004075"
     CDS             381..1088
                     /locus_tag="ACHKIK_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO9085147.1"
                     /translation="MNTAIFNDKASMTSVEIAELVGSQHKDVKRSIERLMGKGVIRSA
                     PMANFEIINNLGLKRNVAAYVFEGEQGKRDSIIVVAQICPEFTARLVDRWRELEEQIR
                     KPMSEIEMVAAMALEAVRQQKRITQVEEKVIHVAETVEQIKKGTIREGYAGYRQLKAK
                     TGLSDDKCCNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMAAFYRVMEEAEPRGT
                     RWYHPKMGLFQVIGWQR"
     gene            1197..1859
                     /locus_tag="ACHKIK_004076"
     CDS             1197..1859
                     /locus_tag="ACHKIK_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO9085148.1"
                     /translation="MIISRPNIDSAISGRYTVSAPYKAGAGGRSPELLEAIYDAPASF
                     LSSMLAHARIMVGWAGEPKGSPVSLSAGTANPVQFTTHEFRSSGGGSFSLLKEAAIMA
                     TVPTSPYLKIEVVNGKAVIFSLHVACHFKRMHQNIVDKIEYLNCSREFFTRNFIPGTY
                     HIYGDSLRGYYITLDGLMMLQLGLSLRTMRYYESCIEAFHEAEISLSHTAFRRNQWEV
                     RP"
     gene            1856..2089
                     /locus_tag="ACHKIK_004077"
     CDS             1856..2089
                     /locus_tag="ACHKIK_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085149.1"
                     /translation="MIRRVVNSLYHRYNRCPRVGQWFTTSNGHVLRVCLVNTESQMVV
                     CQVQGRTHTLSYPLVAFQSGKMFKRLGGGYASV"
     gene            2076..2984
                     /locus_tag="ACHKIK_004078"
     CDS             2076..2984
                     /locus_tag="ACHKIK_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9085150.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETTRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARI
                     REGVTPETMLNGVKRYAAWVRVSGNTGTQFVKQASTFFGPDRHFEDFWQQPAAPGGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            2995..3873
                     /locus_tag="ACHKIK_004079"
     CDS             2995..3873
                     /locus_tag="ACHKIK_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO9085151.1"
                     /translation="MMTFNLREQQTRLQARMDELRAEIAFAQKGEKPWPYRSCWMREG
                     RGCCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSLKAEELTDNA
                     GIALRFRDCEFDNYLEVNPDAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVSMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADDVIEHYASLDLLIIDEV
                     GVQFGSAAEMAILQEIINARYEGILPTILISNLSPEELWAFISPRIADRITNGGRNWL
                     SFNWPSYRSRIGGVAA"
     gene            3870..5270
                     /locus_tag="ACHKIK_004080"
     CDS             3870..5270
                     /locus_tag="ACHKIK_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO9085152.1"
                     /translation="MTSQNTPAWRNDDLEGAVIGAFFLRGADPEVMDILATLSADVFS
                     ARQYRDIYAGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAHTCEAAEDALKDAQNMMASLSTGKGVIQPVHIDDVL
                     PEVVDRVECRNQGLEKSRALMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVARFEDEDWARFSQGVGR
                     MTGRNIWMVDQANLTIDEICATTRHHRMKHPETALVVVDYLGLIKTRSTGRHDLAVGE
                     ISKGLKSLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPETQARGIAEINITKQRNGTLGTIYRRFHNGHFLPVDQESARVLSTPMTPGNPR
                     RYSNNRMSGSKTERLF"
     gene            5267..5524
                     /locus_tag="ACHKIK_004081"
     CDS             5267..5524
                     /locus_tag="ACHKIK_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085153.1"
                     /translation="MNNRTTTVSPEQLRRQAQEMLRCAEQMEKTSVAKDTLRKQLTPA
                     LRDLLQAKHRTQKAVDELVDCVAELEGQVSQFETLVKEFTA"
     gene            5576..6565
                     /locus_tag="ACHKIK_004082"
     CDS             5576..6565
                     /locus_tag="ACHKIK_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9085154.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRHEAVIRRAGGVECLESWLLREKGCQWPHSGWHSE
                     NMTTMRHAPGAIRLCWHCDNLLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVLPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            6584..6964
                     /locus_tag="ACHKIK_004083"
     CDS             6584..6964
                     /locus_tag="ACHKIK_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO9085155.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSTTKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            7180..7377
                     /locus_tag="ACHKIK_004084"
     CDS             7180..7377
                     /locus_tag="ACHKIK_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO9085156.1"
                     /translation="MLKQQDMTETARVVFNELSATAPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            7529..8587
                     /locus_tag="ACHKIK_004085"
     CDS             7529..8587
                     /locus_tag="ACHKIK_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO9085157.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWTGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYLKLQSLFARVA
                     EEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAALALGRRATGVELET
                     ERFEQTVREVQDLASQNG"
     gene            8628..8703
                     /locus_tag="ACHKIK_004086"
     tRNA            8628..8703
                     /locus_tag="ACHKIK_004086"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8661..8663,aa:Ile,seq:cat)
     gene            8713..8789
                     /locus_tag="ACHKIK_004087"
     tRNA            8713..8789
                     /locus_tag="ACHKIK_004087"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8747..8749,aa:Arg,seq:tcg)
     gene            8803..8879
                     /locus_tag="ACHKIK_004088"
     tRNA            8803..8879
                     /locus_tag="ACHKIK_004088"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8837..8839,aa:Arg,seq:tct)
     gene            8970..9929
                     /locus_tag="ACHKIK_004089"
     CDS             8970..9929
                     /locus_tag="ACHKIK_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO9085158.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            9941..10210
                     /locus_tag="ACHKIK_004090"
     CDS             9941..10210
                     /locus_tag="ACHKIK_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFO9085159.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(10508..10771)
                     /gene="iraM"
                     /locus_tag="ACHKIK_004091"
     CDS             complement(10508..10771)
                     /gene="iraM"
                     /locus_tag="ACHKIK_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO9085160.1"
                     /translation="MWCNIKLILWYQSDIFLPPGCIFTPTRTGVILNNKELPVTIYNV
                     TPFNKNVWNLIKSSQECPANTGNITDKCFNHRCILEICPYGRK"
BASE COUNT         2720 a         2467 c         2957 g         2629 t
ORIGIN      
        1 gatgtattct tgatttgtaa agttaatgtt ggagctgcgc tgtgataaaa ctgaatgatt
       61 acgacatgtt gcgtaaattg atccctcaaa acgccatagc gagatacatc ggtgttacgc
      121 cacaagcggt gaatctgtgg ttttctaaaa actccgttcc ttctcgtttt gttttacggg
      181 tgtgtgaatt ggttgaatgg aaggttactc cgcatgggtt aagacctgat ctataccctt
      241 atcccgaaga tggaattcct gattcgttac gaaaatcaaa cggtattacc agagattaaa
      301 gactgactgt cacaagcagt aattcacgat gaatacgttt tggcgtgagt tctgaaatgt
      361 catcctcaag gagcaatcag atgaacaccg caatttttaa cgataaagca tccatgacca
      421 gcgttgagat tgcagagctg gtgggcagcc aacacaaaga tgttaaacgc agcattgaaa
      481 gactcatggg gaagggagtt atccgaagtg cgccaatggc gaatttcgaa ataatcaaca
      541 acttaggatt gaaaagaaat gtagctgctt acgtattcga aggtgaacaa ggcaaacgcg
      601 acagtatcat tgttgtcgcg cagatttgcc ctgaattcac cgcccgcttg gtagaccgct
      661 ggcgtgaact ggaagaacag atccgtaagc caatgagcga aatcgaaatg gttgccgcga
      721 tggctcttga agccgttcgc caacagaaac ggatcactca ggtggaagaa aaagtcatcc
      781 acgttgctga aacagttgag caaattaaaa agggcactat tcgtgagggc tatgccggat
      841 atcgccaact gaaagcaaaa accggtttgt cagatgataa atgctgcaat ctggtgaacg
      901 cctatcaaat tcctacagac acacatgagt tcatgacgcc ggacggattg ttgtcacgtc
      961 gcgcaattgt tgctgtggaa ccgtttatgg ctgcttttta tcgggttatg gaggaagcag
     1021 aaccgcgagg gactcgctgg tatcacccga aaatggggtt atttcaggtt attggttggc
     1081 agcggtgaaa aaaagccggg agtaacccgg ctcactcaac atcaataacg gggagctgtt
     1141 tcgcataaaa cggctccgaa acatccaaga acagttctaa agatatcagc agctatatga
     1201 tcatttcaag accaaatatt gattctgcaa tttcgggacg ttacactgtc tccgcacctt
     1261 ataaagcggg tgccgggggt cgcagcccgg aattgctcga ggcgatatac gacgcgccag
     1321 cgtctttttt atcgtccatg ctcgcgcacg ccagaattat ggtgggctgg gcaggggagc
     1381 cgaaaggctc gccggtttcc ttgagcgccg gtactgcgaa ccctgttcag ttcaccaccc
     1441 atgagtttcg cagctccggt ggtggaagtt tttcactgct caaggaggct gccatcatgg
     1501 ctacagtccc aacttcccca tacctgaaaa ttgaagttgt caacggcaag gccgttattt
     1561 tctccctgca tgttgcctgc cactttaagc gcatgcacca gaacatcgtc gacaaaatcg
     1621 agtatctgaa ctgctcacgc gagtttttta cccgcaattt cataccgggt acttatcaca
     1681 tctacggtga ctccctgcgt ggttattaca tcacccttga tggtctgatg atgcttcagc
     1741 ttgggttaag tctacgcaca atgcggtact acgagagctg cattgaagca ttccatgagg
     1801 cagaaatcag cctcagtcac accgctttcc gccgtaatca atgggaggtg cgcccatgat
     1861 tcgccgcgtc gttaattccc tgtatcaccg atacaaccgt tgcccccgtg tggggcagtg
     1921 gttcaccacc agcaacggcc acgttctgcg ggtttgcctg gtcaatacag aaagccagat
     1981 ggttgtctgc caggttcagg gacgtactca taccctgagt tatccgctgg tggcttttca
     2041 gtccgggaaa atgtttaagc gtctgggagg tggctatgcg tccgtctgat cttctgctcg
     2101 attttgggca tccggttgct tattaccctg ggctcgttaa atacatggga agtccgcacg
     2161 ctgttatttt ctttggtcag attttttact ggcaggataa agcacatgca gcggaaggcg
     2221 tacataaaac gcgtgaagag atacaacacg aaactggact tacatttgaa caacaggctg
     2281 tagcgcgtaa gcatcttgtg tccagaggca ttttggttga aaccaacaag cgtcttgagc
     2341 acaaaatgtt ctaccgtata gattgtgagc gccttaatga aattatcaat gaaaacaatc
     2401 agttttcccg aaatggggaa acccgttttc gggaaactgt aaaacccaat ttcgcggagg
     2461 agggaaagcc ttcaccgcgg acacgggaaa ccactcgccg cggagaaggg aaaaccaatt
     2521 tcgatcttac agagaataca acagagatta cttcagagaa tactacagag agtaaaaaca
     2581 ctattggcgc atccgctgac gcgtctgcac cagcgcgttc tgcccgacag gaatattcac
     2641 cggaatttga acaggcctgg caggaatatc ccaaacgtgc tggtggtaat tccaagtcag
     2701 cagccttcaa agcctggaaa gcccgaatca gggaaggtgt gacaccagaa accatgctca
     2761 acggtgtgaa acgctatgct gcctgggtgc gtgtctcagg aaataccggc acccagttcg
     2821 tgaagcaggc gtcgacgttc tttggacccg atcgtcactt cgaagatttc tggcaacagc
     2881 cagcagctcc cggaggtggg cgacagcgac aggtcgatgt cctggctggc ctgggagcca
     2941 tgtctgacaa attcggtaaa tccagtgaca aattgacatt ctgaggtgac agcgatgatg
     3001 acgtttaacc tgcgtgaaca acaaacaaga ctacaggcgc ggatggatga gttacgggca
     3061 gagattgcat ttgctcagaa gggcgaaaaa ccatggcctt atcgttcctg ctggatgcgt
     3121 gaagggcgcg ggtgttgcga aaaacatggc gaataccaca cacatattct ggtgtggagc
     3181 gatcgtaatg gcgaggacag agaaaaaatt tcatgctgcc ctgactgctt gatagctgag
     3241 gccaacgatt tgaccatgga gctgtcgtcc ctcaaggcgg aagaactgac tgataacgcc
     3301 ggaattgctc tgcgttttcg ggactgcgag tttgataatt atctggaggt taatcctgac
     3361 gcagccagaa atcttgcggc ctgtcgccgc tatgcggaga actggccaga tatgctggag
     3421 aacggtacca gtcttgttat gaccggcagt tgcggtaccg ggaaaaatca tctggcggta
     3481 tcaatggcaa aacacatcat ccgtaactat ctggccagtg tggagatcac cgacgtgatg
     3541 cgccttaccc gtgctgtgaa aaactgctgg cggaatgaca gtgaaaaaac agcggatgac
     3601 gtcattgagc attatgcgtc actggatttg ctgatcatcg acgaagtcgg cgttcagttt
     3661 ggcagtgcgg ctgaaatggc cattttgcag gaaattatca atgcccggta tgagggtatt
     3721 ttgccaacta tcttgatcag caacctttca ccggaagaat tgtgggcgtt catcagtccc
     3781 cggattgccg acaggatcac caatggcggg cgcaactggt tgtcgtttaa ctggcccagc
     3841 taccgttctc gtatcggagg tgttgccgca tgaccagcca gaacaccccg gcatggcgta
     3901 acgatgacct ggaaggcgct gtcattggcg cgttttttct gcgtggggct gatccggaag
     3961 tgatggatat tctggccaca ttgtcggcgg acgttttttc tgcacgacag taccgggata
     4021 tttacgcggg aatttgcaga caggcacgtg tatccggcgt cattgacccc gtactgctgt
     4081 gcaatgagat gccggaactt gccccggtga ttaccgacac cggacgcaaa acctgggtga
     4141 agtcttcact ggagcactat gtcgcagcgt tgcggcgtaa tgccgcactg cgcgatgcag
     4201 aaaaaacact gactgaagca ttacagaatt tacgggatgc gcatacctgt gaagcagccg
     4261 aggatgccct gaaggatgcg cagaacatga tggcctcact gtcgaccgga aagggcgtca
     4321 ttcagccggt tcacattgat gatgtccttc cggaagtggt cgaccgtgtt gaatgccgca
     4381 atcagggact ggagaaatcc agggcgctga tgaccggtat tgatgaactg gacgcaaaaa
     4441 cgggcggtat ggagccaggc gacctggtat tcattgcggc ccgtccttcg atggggaaaa
     4501 ccgaacttgc gctggatatc atcgacaagg tgactgagca ggggcatggc gtgcttctgt
     4561 tcaccatgga gatggcgaac attcagattg gtgaacgtat ggtgtctgct gccgggggaa
     4621 tgccggtatc ccgtcttaag tctgttgccc gttttgaaga tgaagactgg gcgcgtttct
     4681 cacagggcgt gggacgaatg acggggcgta atatctggat ggtggaccag gcaaacctga
     4741 ccattgatga gatatgtgca accacgaggc accaccggat gaaacacccg gaaacggcgc
     4801 tggtggtggt cgattacctc ggcctgatta aaacccgcag cacggggcgt cacgaccttg
     4861 ctgtggggga aatctcaaag ggacttaaaa gcctggcaaa atccggcggt tttccgctga
     4921 ttgctctgag ccagctctcc cgtggcgtgg aatccagacc caataaacgc cccatgaact
     4981 cggacctgaa aaactcaggg gaaatagagg cggatgcaga catcattctg atgctttaca
     5041 gggatgaggt gtacaacccg gagacacagg cgagaggcat agctgaaatc aacatcacga
     5101 aacagcgtaa tggcacgctg ggtaccattt accggcgttt tcataacggg cattttctgc
     5161 ctgtggacca ggagagtgcc cgggttcttt ccactcccat gacgccgggc aatccgcgca
     5221 gatacagcaa taaccgcatg tcgggtagta aaacggagcg tttattttga acaacagaac
     5281 caccactgtt tcaccggaac aacttcgtcg gcaggcgcag gagatgcttc gttgtgctga
     5341 acagatggaa aaaacgagcg tggcaaaaga tacgctccgc aagcagctta ctccggcgct
     5401 tcgtgatctg ctgcaggcaa aacatcgcac acaaaaggcg gtggatgagc tggtggattg
     5461 cgtggcggaa ctggaaggcc aggtaagcca gtttgaaacg ctggtgaaag agtttactgc
     5521 gtgatggctg aattttttct cctgcgttca tgcaataccg ttcgctgagg tgaccgtgag
     5581 agcactgctg acccctgaaa ttgccccgcg tatggggatc gtcttgttca gacccggttc
     5641 agagctgatg cccctgttta tgcaggggcg tgtcctgctg gagcctgagc cggaacgtta
     5701 ttcatctttt gccagtggtg ccgttccggc agcatcacaa ccgctggcgg atgatcctgc
     5761 cgttcgggcc gtgttccgcc atgaggcggt gatccgtcgt gctggtggcg tggaatgcct
     5821 tgagagctgg ttacttcgtg aaaaaggctg ccagtggcct cattccggat ggcacagcga
     5881 gaacatgacc acaatgcgac acgcgccggg cgcaatccgt ctgtgctggc actgcgataa
     5941 cctgcttcgc gatcagttca cggaacgtct ggagtcaatg gcaacggata actgtgcccg
     6001 ctgggtgttg tccgtagtcc gtcgtgatct cggttttgat gacagtcacg ttgtgacaat
     6061 gcctgaactg tgctggtggc tggttcgtaa tgacctggcg gatgccttac cggaaagtgc
     6121 agcccgtaag gccctgagat taccgaagcc tgttttgccg tctgtcaccc gggagagtga
     6181 ccttgtgcct tcggttcctg ccaccagcat tatccaggat aaagcgaaaa aggtgctggc
     6241 gctgaaagtg gatccggagt cgccggagtc ttttatgtta cgccccaaac gtcgtcgctg
     6301 ggttaatgaa aagtacacgc gctgggttaa gacacagcct tgcgcgtgtt gtggtaagcc
     6361 agccgacgat ccacatcacc tgattggcca tggtcagggt ggaatgggta caaaagcgca
     6421 tgaccttttt gtgttgcctt tgtgcagaaa acaccatgac gaactgcatg cggataccgt
     6481 ggcatttgaa gagaagtatg gttcccaact ggagctgata tttcgtttta tcgatcgcgc
     6541 actggcgatt ggtgtgctgt cctgattttt gtggagaaag ttgatgcgtg atattcagat
     6601 ggttcttgaa cgctgggggg catgggcggc aagtggtaac accggggtgg actattctcc
     6661 gatcgctgcc ggattcaaag gccttttacc atctaccact aaaccacgtc cagcctgctg
     6721 cgatgatgac ggacttatca ttgaaaactg tcttgcacgt ctgaagcaga aaaaacctga
     6781 ggagtattcg cttctcattg ctcattattt gttgcgaata tcaaaaagac agatagccag
     6841 gacgagaaag aaaagcgaaa aagcaatacg aattgagatg cagatagccg aagggtttat
     6901 tgacggatgt ttgtctgtgc tgggggtaag actggagatg gacgactggc tgctaaaaaa
     6961 gtaaaaaatg attagtgcgg tccgcaaaaa gtatgtcagt atgttaagag tggttacttt
     7021 gccacacagc ttaaatccgc cgtcaggcgg gttttttatg cctgaaatcg gaccagtacg
     7081 ttaaacgcgc tggtggcggt gaataccggt ctttcagctt gctggctttt tcgacaagag
     7141 ttattggtgt gtcacgttaa ccggaaaagg gaaaaagaca tgctgaaaca gcaggatatg
     7201 acagaaaccg ccagagtggt gtttaatgaa ttaagtgcca ccgcaccggc gacagtcggg
     7261 gagattgcac agaatacgta cctttcacgc gaacgctgcc agctaatact gacccagctt
     7321 gttatggcgg gtctggcaga ctatcagttt ggttgttaca gacgccttca gtcctgaagg
     7381 cttttttatt tgtggtaaat gggcggctgg tgggtgttag gggcactcac cagccatctg
     7441 ctcatgcgtt ggggtcacaa gcaaacctca ggcccatctg ctttgcgcaa aagcggtatg
     7501 agcctatcag agacaggctt aatgatccat gtttaatact gtaaaaatat ccagttgtga
     7561 gttaatcaac gccgactgcc tggaatttat ccggtcgtta cccgaaaatt ctgttgacct
     7621 gatagtcacg gacccgccgt actttaaagt gaagcctgag ggctgggata accagtggac
     7681 gggtgatgag gattacctga aatggctgga ccagtgtctg gcgcagttct ggcgggtgct
     7741 gaaacctgcc ggaagtcttt acctgttctg tggccatcgc ctggcatctg acattgaaat
     7801 catgatgcgt gaacgcttca gtgtgctgaa ccatattatc tgggcgaagc cgtccggacg
     7861 ctggaacggg tgcaacaagg aaagcctgag ggcgtatttc cccgccacag agcgcattct
     7921 gttcgcggaa cattatcagg ggccgtatcg tccgaaagat gccgggtatg aggcgaaggg
     7981 cagggcactg aaacagcatg tgatggctcc gctgattgct tactttcgtg atgcgcgtgc
     8041 tgccctgggg ataacggcaa aacagatagt ggatgccaca ggaaagaaaa acatggtgtc
     8101 gcactggttc agtgccagtc agtggcagct accgaacgaa agcgattatc tgaaattaca
     8161 gtcgctgttt gcccgggtgg cagaagagaa gcatcagcgc ggtgaactgg aaaagcccca
     8221 ccaccagctg ctggagacgt atacttcact gaaccggcag tatgcggaac tgcagagtga
     8281 atataagcat ctgcggcggt attttggcgt gacggcgcag gtgccgtaca cggatgtgtg
     8341 gacgcataaa ccggtgcagt actatcccgg gaaacatccg tgcgaaaaac cggcagaaat
     8401 gctgcagcag ataatcagcg cgagcagtcg tccgggtgac ctggttgcag attttttcat
     8461 ggggtcgggt tcgacagtca aagctgcact ggcgctgggg cgtcgtgcaa ctggcgttga
     8521 gctggagact gaacgttttg agcagacggt cagggaagtt caggatttag ccagtcagaa
     8581 cggatgatat tgcaggatta gttacgtacc gttattatcc tgcgcccggc cctttagctc
     8641 agtggtgaga gcgagcgact cataatcgcc aggtcgctgg ttcaaatcca gcaagggcca
     8701 ccatatcaca taccgccatt agctcatcgg gacagagcgc cagccttcga agctggctgc
     8761 gcggggttcg agtcctcgat ggcggtccat tatcggtatt cagcgttgtt agctcagccg
     8821 gacagagcaa ttgccttcta agcaatcggt cactggttcg aatccagtac aacgcgccat
     8881 atttatttac caggctcgct tttgcgggcc ttttttatat ctgcgccggg tctggtgctg
     8941 attacttcag ccaaaaggaa cacctgtata tgaagtgtat attatttaaa tgggtactgt
     9001 gcctgttact gggtttttct tcggtatcct attcccggga atttacgata gacttttcga
     9061 ctcaacaaag ttatgtctct tcgttaaata gtatacggac agagatatcg acccctcttg
     9121 aacatatatc tcaggggacc acatcggtgt ctgttattaa ccacaccccg ccgggcagtt
     9181 attttgctgt ggatatacga gggcttgatg tctatcaggc gcgttttgac catcttcgtc
     9241 tgattattga gcaaaataat ttatatgtgg ccgggttcgt taatacggca acaaatactt
     9301 tctaccgttt ttcagatttt acacatatat cagtgcccga tgtgacaacg gtttccatga
     9361 caacggacag cagttatacc actctgcaac gtgtcgcagc gctggaacgt tccggaatgc
     9421 aaatcagtcg tcactcactg gtttcatcat atctggcgtt aatggagttc agtggtaata
     9481 caatgaccag agatgcatcc agagcagttc tgcgttttgt cactgtcacg gcagaagcct
     9541 tacgcttcag gcagatacag agagaatttc gtcaggcact gtctgaaact gctcctgtgt
     9601 atacgatgac gccgggagac gtggacctca ctctgaactg ggggcgaatc agcaatgtgc
     9661 ttccggagta tcggggagag gatggtgtca gagtggggag aatatccttt aataatatat
     9721 cagcgatact gggtactgtg gccgttatac tgaattgcca tcatcagggg gcgcgttctg
     9781 ttcgcgccgt gaatgaagag agtcaaccag aatgtcagat aactggcgac aggcccgtta
     9841 taaaaataaa caatacatta tgggaaagta atacagctgc agcgtttctg aacagaaagt
     9901 cacagttttt atatacaacg ggtaaataaa ggagttaagt atgaagaaga tgtttatggc
     9961 ggttttattt gcattagctt ctgttaatgc aatggcggcg gattgtgcta aaggtaaaat
    10021 tgagttttcc aagtataatg aggatgacac atttacagtg aaggttgacg ggaaagaata
    10081 ctggaccagt cgctggaatc tgcaaccgtt actgcaaagt gctcagttga caggaatgac
    10141 tgtcacaatc aaatccagta cctgtgaatc aggctccgga tttgctgaag tgcagtttaa
    10201 taatgactga ggcataacct gattcgtggt atgtgggtaa caagtgtaat ctgtgtcaca
    10261 attcagtcag ttgacagttg cctgtcagac tgagcatttg ttaaaaaaat ttcgcatggt
    10321 gaatccccct gtgcggaggg gcgactggtg tataaggtgt catctctgac gacaacctgg
    10381 atcgagatac gcgggttcag ggacaccgga ctgaactcac cgggaggcac ccggcaccat
    10441 gttcatggtg atacagaaat gcggcttcag cccctctccg gaggggcttt ttatctgaat
    10501 gattctgtta ttttcgcccg tatgggcata tttccagaat gcagcgatga ttaaaacact
    10561 tatctgtgat atttcctgtg tttgcagggc attcctggct gcttttaatt aaattccaga
    10621 cgtttttatt aaatggggtc acgttgtaaa tggtgacggg gagctcttta ttattgagta
    10681 ttacgcctgt ccgggttggt gtaaaaatac agcccggagg gaggaagata tcagactgat
    10741 accataatat taattttatg ttacaccata tgg
//
LOCUS       DAZGJW010000152        10753 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZGJW010000152 DAZGJW010000000
VERSION     DAZGJW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.152"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..809
                     /gene="hlyE"
                     /locus_tag="ACHKIK_004092"
     CDS             <1..809
                     /gene="hlyE"
                     /locus_tag="ACHKIK_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFO9085161.1"
                     /translation="PWQTFDETIKELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEA
                     TQTVYEWCGVATQLLAAYILLFDEYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVS
                     SQSFNNASGKLLALDSQLTNDFSEKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIIS
                     YSIAAGVVEGKLIPELKNKLKSVQNFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEI
                     KTETETTRFYVDYDDLMLSLLKEAAKKMINTCNEYQKRHGKKTLFEVPEV"
     gene            complement(1016..1462)
                     /gene="ycgN"
                     /locus_tag="ACHKIK_004093"
     CDS             complement(1016..1462)
                     /gene="ycgN"
                     /locus_tag="ACHKIK_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO9085162.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1554..2213)
                     /gene="ycgM"
                     /locus_tag="ACHKIK_004094"
     CDS             complement(1554..2213)
                     /gene="ycgM"
                     /locus_tag="ACHKIK_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO9085163.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            complement(2285..2578)
                     /locus_tag="ACHKIK_004095"
     CDS             complement(2285..2578)
                     /locus_tag="ACHKIK_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO9085164.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            complement(2590..2748)
                     /locus_tag="ACHKIK_004096"
     CDS             complement(2590..2748)
                     /locus_tag="ACHKIK_004096"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085165.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            2819..3220
                     /gene="pliG"
                     /locus_tag="ACHKIK_004097"
     CDS             2819..3220
                     /gene="pliG"
                     /locus_tag="ACHKIK_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO9085166.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3323..3691)
                     /gene="ycgJ"
                     /locus_tag="ACHKIK_004098"
     CDS             complement(3323..3691)
                     /gene="ycgJ"
                     /locus_tag="ACHKIK_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO9085167.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDASGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            4211..4906
                     /gene="minC"
                     /locus_tag="ACHKIK_004099"
     CDS             4211..4906
                     /gene="minC"
                     /locus_tag="ACHKIK_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO9085168.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4930..5742
                     /gene="minD"
                     /locus_tag="ACHKIK_004100"
     CDS             4930..5742
                     /gene="minD"
                     /locus_tag="ACHKIK_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO9085169.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5746..6012
                     /gene="minE"
                     /locus_tag="ACHKIK_004101"
     CDS             5746..6012
                     /gene="minE"
                     /locus_tag="ACHKIK_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO9085170.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6384..6728)
                     /locus_tag="ACHKIK_004102"
                     /pseudo
     CDS             complement(6384..6728)
                     /locus_tag="ACHKIK_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6763..6882)
                     /locus_tag="ACHKIK_004103"
     CDS             complement(6763..6882)
                     /locus_tag="ACHKIK_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO9085171.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(6843..7028)
                     /gene="ymgJ"
                     /locus_tag="ACHKIK_004104"
     CDS             complement(6843..7028)
                     /gene="ymgJ"
                     /locus_tag="ACHKIK_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFO9085172.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            7129..7302
                     /gene="ymgI"
                     /locus_tag="ACHKIK_004105"
     CDS             7129..7302
                     /gene="ymgI"
                     /locus_tag="ACHKIK_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO9085173.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            7304..7648
                     /gene="ymgG"
                     /locus_tag="ACHKIK_004106"
     CDS             7304..7648
                     /gene="ymgG"
                     /locus_tag="ACHKIK_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO9085174.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            7652..7987
                     /gene="ymgD"
                     /locus_tag="ACHKIK_004107"
     CDS             7652..7987
                     /gene="ymgD"
                     /locus_tag="ACHKIK_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO9085175.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(8044..10365)
                     /locus_tag="ACHKIK_004108"
     CDS             complement(8044..10365)
                     /locus_tag="ACHKIK_004108"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO9085176.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     GSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(10482..10691)
                     /locus_tag="ACHKIK_004109"
     CDS             complement(10482..10691)
                     /locus_tag="ACHKIK_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085177.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
BASE COUNT         2972 a         2573 c         2356 g         2852 t
ORIGIN      
        1 tcccctggca gacctttgat gaaaccataa aagagttaag tcgctttaaa caggagtatt
       61 cacaggcagc ctccgtttta gtcggcgata ttaaaacctt acttatggat agccaggata
      121 agtattttga agcaacccaa acagtgtatg aatggtgtgg tgttgcgacg caattgctcg
      181 cagcgtatat tttgctattt gatgagtaca atgagaagaa agcatccgcc cagaaagaca
      241 ttctcattaa ggtactggat gacggcatca cgaagctgaa tgaagcgcaa aaatccctgc
      301 tggtaagctc acaaagtttc aacaacgctt ccgggaaact gctggcgtta gatagccagt
      361 taaccaatga tttttcagaa aaaagcagct atttccagtc acaggtagat aaaatcagga
      421 aggaagcgta tgccggtgcc gcagccggtg tcgtcgccgg tccatttgga ttaatcattt
      481 cctattctat tgctgcgggc gtagttgaag ggaaactgat tccagaattg aagaacaagt
      541 taaaatctgt gcagaatttc tttaccaccc tgtctaacac ggttaaacaa gcgaataaag
      601 atatcgatgc cgccaaattg aaattaacca ccgaaatagc cgccatcggg gagataaaaa
      661 cggaaactga aacaaccaga ttctatgttg attatgatga tttaatgctt tctttgctaa
      721 aagaagcggc aaaaaaaatg attaacacct gtaatgagta tcagaaaaga cacggtaaaa
      781 agacactctt tgaggtacct gaagtctgat aagcgattat tctctccatg tactcaaggt
      841 ataaggttta tcacatttaa aaacacagac gttttttaaa gattgagagt tacctgttga
      901 taataaggta tcgggtaact ctcattatga cagagcgctt tcaaaaacag ggctacgaaa
      961 tggcgagcat ccagacgtat attaagaaag cccccatcaa catctattaa atttatcact
     1021 gcgcccagtc aggtttattt aagatatgat cctgccagtc aatcacttct gattctttca
     1081 ctgcgatatg acgcacagag atacgttcac catgcattgc cgctttcgaa cccgtaagta
     1141 gcggatgcca cgcaggtaaa tctttacctt ccgccagcaa ccgataagcg caggtcattg
     1201 gcagccattc gaatgttggc agattttcac gggttaattt aatgcagtcg ggttcaaact
     1261 cgaaacgacg ttcgtagttc cgacattgac aggttttaat attgagctgg cgacaggcga
     1321 cgttagtgaa gtagatttcg tcggtgtctt catccatcag tttatgcagg caacactgac
     1381 cgcaaccatc acacaacgac tcccattccg catcgctcat ttcgtccagg gttttacttt
     1441 gccagaaagg tacatcgctc atcaggtgtt ccgccattac gttaaaacgc accttataac
     1501 cagtctggca cagcgatgca agttttgccg ccgctttcag gcggcaaaaa gtattacaaa
     1561 acgcgagttg ccaaagaatg cccatcgaaa gtgactgtca gctcatcacc gctttgcaac
     1621 gggccgacgc catcaggcgt gcctgtcagc acaacgtcac cggccttgag ggtaaaaaac
     1681 ttgctcatat aagcgatcag cggaacaatt ttatggatca tgtccgcagt cgtaccttgc
     1741 tgacgttgct cgccgtttac gctcaggccc agcgttgtat tttgcgggtc gccggtaaat
     1801 tccgccgcgg gaataaaccc ggaaagcgga caagagttat caaacgcttt agccttttcc
     1861 cacggctgcc cggctttctt catttttccc tgaacatcac gcaatgtcag atcgagcgcc
     1921 acgccataac cggcaatggc tttgcggaca tgctcttccg tagcctgacg cagcgtcgcg
     1981 ccaatcaaca ccgccagttc gacttcatga tgaactgaac cgaaatcgga tgggatcgcc
     2041 aatggctgcc gcagatcgca cagtgccgtt tctggtttaa taaacagcac tggctcttcg
     2101 ggcactgcgc tgcccatctc tttaatatgt ttggcatagt tactgccaac acagactact
     2161 ttactcaccg gataatccag cagcgcacct tgccagttgt gatgttgata cataacgttc
     2221 ccctcacctt atcgtggata tgaaaacccg cgccgacaaa gataatgcca ccgccggata
     2281 tcggttattt gttagtgtcg tctgttttct gccccatgac ggaaagatgt tgcttcagca
     2341 aatcttcggg tggtggcggt aactgcaaat agtaaccttg ctcggttaat gccagtttaa
     2401 ccttttcaat atcagcattg accagtttct tccgcccatc caacggcaga atcatcgcta
     2461 actgaggctg accaaaacct ttcatcagtt cctcaggaac acgcgaaaaa tcgtcttttt
     2521 tttcgacata taaataggtc tggtcacgct tgctgcttcg ataaatcaca caaaacatac
     2581 ttttactctt taagatgcca ggttttggca taagctaaca tcaatgaaac ccgcgccaca
     2641 agccagccag ctgtagattt ctacggtttg ttgagtccat gcccctttat aagtaatccc
     2701 cccggcaaaa atgccgcgcc gggattttac cgaacgcagg aaagccactg gaattctccc
     2761 gttagggatt gttatattat aacagttcat cgtactcatt ctgaacagga gactaccaat
     2821 gaaaatcaag agcatcagta aggctgtatt gctgtttgca ctgctaacct ccacgagctt
     2881 tgctgcgggt aagaatgtca atgttgagtt cagaaaaggc catagcagcg ctcagtactc
     2941 cggcgaaata aagggatacg attacgatac atataccttc tatgccaaaa aaggtcagaa
     3001 agtgcatgtg agtatctcta atgaaggcgc tgatacttac ctcttcgggc caggaatcga
     3061 tgattcagtt gatttatcca gatattcccc agaactcgat agtcacggac aatactcact
     3121 ccctgcttct ggcaaatatg agctgcgagt gttgcaaacc cgaaacgacg cccgtaaaaa
     3181 caaaactaaa aaatataacg tcgatattca aattaagtaa atgtcaaccg acggggactc
     3241 tccccgtcat aaacgttaac gccccactcc tctctgtcgc tcaagaggtt aatgacgaat
     3301 agaacataat gtaaacaaga catcactcac gacacataaa taacatcttt gtggtggttt
     3361 gattgatttt tccactccgc ttaccatcta caccaaaata acgatcatca cgacataatt
     3421 tttctttaac atcgcaaaat acaccgttag cgaatgtaaa ttcgctggga tcgtagcctt
     3481 gtaatgattg taagttttca gcggcttttt cgccgagata tttccttgtt aactctgggg
     3541 aaatgccatc tgagttcaca caaatatacg ggtcgcaaac aacgccgcac gccggagatt
     3601 gaagtccgct agcgtcatgt ccagacgcca ttgatgagaa catcatcccc acacctgaca
     3661 atccgatata aataatacgc atcatattca tagcacatcc tgataatcca accgtacaac
     3721 acttatgctc atttcgtcat caatccgtaa tcaaaaccgc tgacctggac gagttacgat
     3781 gacctgacag aagtatcaga cgtcttctgc ggtttctatg atagagcaat tctgtggcgg
     3841 atattaagat aacgggtatc tgttctttaa tgatgccgca aaaaatggaa agttaagaaa
     3901 gcctggcgtg gtgaaaaaat acacttgcct caatactgga tatttaaaca ggtctggaga
     3961 tgctgtcacg attagggtgc ggaaaaagga agcgtaaata agaagaatca aaagtacgac
     4021 ggcaatgggt tgattgacaa gggtattttt taagctatga atcagcgcca tttatcacag
     4081 aatagacttt tactctgaat aaatgggagg gtgacttgcc tcaatataat ccagactata
     4141 acatgcctta tagtcttcgg aacatcatcg cgcgctggcg atgattaata gctaattgag
     4201 taaggccagg atgtcaaaca cgccaatcga gcttaaaggc agtagcttca ctttatctgt
     4261 ggttcatctg catgaggcag aacctaaggt tatccatcag gcgctggaag acaaaatcgc
     4321 tcaggccccc gcatttttaa aacatgcccc cgttgtactc aacgtcagtg cactggaaga
     4381 cccggtaaac tggtcagcga tgcataaggc ggtttcggca accggtttgc gggttattgg
     4441 cgtaagtggc tgcaaagatg cgcaacttaa agccgaaatt gaaaaaatgg ggctgcctat
     4501 cctgacggaa ggaaaggaaa aagcgccacg tccagctccc gcaccgcagg ctccagcgca
     4561 aaatacaacg ccggtcacaa aaacgcgttt aatagatacc ccggtgcgtt ccggtcagcg
     4621 tatttatgct ccacaatgtg atctgattgt tacaagccac gttagcgctg gggccgaatt
     4681 gattgccgat ggtaacattc atgtctatgg catgatgcgc ggtcgtgcgc tggcaggggc
     4741 aagtggtgac cgggaaacgc aaatattttg tacgaacctg atggcggaac tggtgtccat
     4801 cgcaggtgaa tactggctga gtgatcaaat cccagcagaa ttttatggca aagcggcgcg
     4861 actgcagtta gtcgaaaacg ctttgaccgt tcaaccgtta aattgatccc tttttaacaa
     4921 ggaatttcta tggcacgcat tattgttgtt acttcgggca aagggggtgt tggtaagaca
     4981 acctccagcg cggccatcgc cactggtttg gcccagaagg gaaagaaaac tgtcgtgata
     5041 gattttgata tcggcctgcg taatctcgac ctgattatgg gttgtgaacg ccgggtcgtt
     5101 tacgatttcg tcaacgtcat tcagggcgat gcaacgctaa atcaggcgtt aattaaagat
     5161 aagcgtactg aaaatctcta tattcttccg gcatcgcaaa cacgcgataa agatgccctc
     5221 acccgtgaag gggtcgccaa agttcttgat gatctgaaag cgatggattt tgaatttatc
     5281 gtttgtgact ccccggcagg gattgaaacc ggtgcgttaa tggcactcta ttttgcagac
     5341 gaagccatta ttaccaccaa cccggaagtc tcctcagtac gcgactctga ccgtatttta
     5401 ggcattctgg cgtcgaaatc acgccgcgca gaaaatggcg aagagcctat taaagagcac
     5461 ctgctgttaa cgcgctataa cccaggccgc gtaagcagag gtgacatgct gagcatggaa
     5521 gatgtgctgg agatcctgcg catcaaactc gtcggcgtga tcccagagga tcaatcagta
     5581 ttgcgcgcct ctaaccaggg tgaaccggtc attctcgaca ttaacgccga tgcgggtaaa
     5641 gcctacgcag ataccgtaga acgtctgttg ggagaagaac gtcctttccg cttcattgaa
     5701 gaagagaaga aaggcttcct caaacgcttg ttcggaggat aagttatggc attactcgat
     5761 ttctttctct cgcggaagaa aaacacagcc aacattgcaa aagaacggct gcagattatt
     5821 gttgctgaac gccgtcgcag cgatgcagaa ccgcattatc tgccgcagtt gcgtaaagat
     5881 attcttgagg tcatttgtaa atacgtacaa attgatcctg agatggtaac cgtacagctt
     5941 gagcaaaaag atggcgatat ttctattctt gagctgaacg tgaccttacc ggaagcagaa
     6001 gagctgaaat aagcccgctg taaaaacgca tttatcttca aggcagagtc atttctgcct
     6061 tgagtttttc atccctctca tccacgttgt ggtaaagcgg cgagtattct tgctgatact
     6121 cctcattgct atttccacct ccccctttac accttaaggc tgttcccacg cacagaacaa
     6181 aatgtttatg cctggttgag taaataacct cattgttagt gtggatacac tgggggatga
     6241 taaccccgca atttcattat tcattgttct cccataggat gaggtttccc gccgtaactg
     6301 gcgggctttt tttgccccag aattttccct tttagccccc tgtaaacgaa aactgcgccg
     6361 aagcgcagtt taatcgttga tggtcagaac gaccagttca caccagccac cgcattccac
     6421 ggggattcca caccggcacc atggctatac cccaccccga aatgcccgct taacgtactg
     6481 ctgaatgagg ctttaatacc tgcctggtat attccacgtc tgcccgacaa atcattgaca
     6541 aaattaccgt cactattcac tttcacccga ttatcatcga caaattcttt gctcacagcc
     6601 gccttcaggc agggttcaat ttccataccg ttccccagac gcatgttgta actcagcgtt
     6661 gcacccagtt cacgatatat actgcgggta tcgactgatt tcgattccat gccattggat
     6721 aaatgatagt agcgtctaag tgacctaagc tctgctgcaa catcagcccg caggcaccag
     6781 accaggggat tcatcctgaa gagacagcgc aggtgtattg tgttcaccgc tcaccaaaga
     6841 catcatgatg aaatgatgat atcccgcata agaaagaggc attttttaca cgcagtgcgc
     6901 tgaagtgtag ttggataaaa aatcactctt ttcagataat actgtcgtac gcttatcttt
     6961 cgacaatgag gccgtcggca aaataaaatg actaatgtaa tcgttgctga tgaatatctt
     7021 ctgctcacat aaaaatcaca caataatatt gataaatcag attatcttac ctttacaaca
     7081 ttgcgttaac agtcaatgac acttcttgaa taaaaatatt gtcaatcaat gagttattcc
     7141 tcatttaaga ttattctcat tcacgtaaaa aacataattc ctatcatcac agccacgctc
     7201 attttgaatt atctgaataa tagcgagcgt tctttagtta agcagatctt aattgaagac
     7261 gaaattattg tgtgtgcaac ttacttaata ccggacatct aagttgaaga aaaaaatact
     7321 tgccttcggg cttatctcag cattgttctg ctccacgcca gcgatggcgg atatgaatcg
     7381 caccacgaaa ggtgcattgc taggcgcagg cgttggttta cttactggca atggtgttaa
     7441 tggcgtactg aaaggtgcag ctgttggcgc tggtgttggt gcagtaacag aaaaaggccg
     7501 cgacggtaaa aatgcgcgta aaggtgcaaa agtaggtgcc gccgttggtg ctgtcactgg
     7561 cgttctgacc ggcaatggcc tcgaaggggc gattaaaggt gccgtgattg gcggtacggg
     7621 tggtgcaatt ctgggtaaaa tgaaataagg aatgatgatg aaaaaatttg ctttactggc
     7681 gggcctgttt gttttcgcac caatgacctg ggcgcaggac tacaatatta aaaacggttt
     7741 gccgtcagaa acctatatta cttgcgcaga agccaacgaa atggcaaaaa ctgacagcgc
     7801 acaggttgca gaaattgttg cggtaatggg taatgccagc gttgccagcc gtgatttaaa
     7861 aattgagcaa tctccggaac tgagcgccaa agtggtagaa aaactgaatc aggtttgcgc
     7921 taaagacccg caaatgctgt tgatcacggc aattgatgac accatgcgtg ctatcggcaa
     7981 aaaataatat ttcatatgta ccgcgtcgat gaatacgacg cggtttttta gctaacggcg
     8041 ggatcagaac ttatatttca ggcccaccat cactgcggtg tcattgtagc cattatcacc
     8101 cagctgcacg cccacattcc cccagacgct ggccgctgga ttcaggttgc catttacgcc
     8161 aagtttaatt tcccccaaat tactcgcccc atcttgttcg actttcacac cgttcattga
     8221 gacagcaaaa tctttactgt tatggatcca gttcatttca acaaaaggtt caaagtcgtc
     8281 accgtgcgga ccgctgtttt tctcctgagt acgaataaag gtgcggaaac caagtcgggt
     8341 ctggatgttg ttatttgcgt cattctcaat acgtgagcca ttggcttcgg tgtgcgcatt
     8401 atgatcgacg cccatccaga tagcctgtgc ctgtggctgg aatatccacg tgtatttcgc
     8461 cgcttccaga ccaaaggatt tattcagggc aaagccgtaa cccgcttcca gcgagacggt
     8521 tgcgcctttc gcactatagg attcataggc taattcatcc cctttcaccg agtttttaaa
     8581 ccagctatat tgcgcccagg cgtcaatgta tgcgcctttt ttactgatgt catcggcaaa
     8641 ccaagtggca tacagccctg cgcaatagcc tctgacactc cctttcgaac gataatccga
     8701 cacgctggaa tgagttgagt tgtagtcgcg ggcataacca gccatcactc ccaaacgcca
     8761 actgtcactg tcggtaaaac caccggttaa cagctcggcc cctaactgcg agacgtagcg
     8821 atgcgaggtc gttctcaact gtccgttgct gtcacgccag gcattatgcc cgccaatttg
     8881 acgtagccaa agtcggcttg aacgctcctg ttcagttaca ggatcgatgt agcgcatttc
     8941 acccgcacgg tcgttcagac gcataacaaa aagagtgtta gcggctgcca ggttggcgac
     9001 gtaacttcca gcttcgaggc gcaggacggg ggtaggcgca acagtaggcg ctgaacttcc
     9061 tccggagttt gtttcaccgc cgctcgttgc ctggaagtta gtcagatacc agtttttatt
     9121 ggtcgcttcg gtattacctc gggttaatga atattcgtag gcaccggcga aaatcctcga
     9181 atccttaata aattccccat ttgattcccc ctcaacgctg ataatctcta tcccatttaa
     9241 cgtgtaagct ccgctgcctc ctgcattggt aactttaact cgggttgttc cagaggtatt
     9301 tccattaata acgactttat ccgtagctga attatcatca cccaattcag ttctgagatg
     9361 caatacgcca ttattaccaa cgtaattttc agttattgtt aatcttgttg gttcgacatc
     9421 ccgtccatct gcccgggaaa tatagaccgt actattatca accgtgaggt tgctcaccgt
     9481 tgagtcagct ttaatatccc aggtactatt atctgaaagc gacagatagg tatgagtgtt
     9541 aacatctgtt gaaatattag ctgaacccgt tatttttgaa ttagttgcat cgacggtcaa
     9601 atgaccttta atattattga taaaaacatc tgcagccgat gatacctcac tatccgtaat
     9661 ggtcacgtta ctcgtctcat ttttatggta gaggatttca tttgctgacc ctagtgagct
     9721 accagataaa atatctaccg ttgcttcctg accatttatg gaagactcca gcacaattcc
     9781 agacccctga tgcgcgactg cagatgtatt tttcagcgtc accgtgccgc cattataaat
     9841 tttagcagtc gcggcgtcag gacccgttgt ataaatttct ccctggttaa atgaaactgt
     9901 cccctggcgg gcagcgatgg catgagcatt gtccccatgc gtggtgatat caacatcggt
     9961 taatgtaacg ctggcattgg tataatcaag cgtgtcacca gccttgacga cagtgccatt
    10021 agcgtacgcg gcataacttt ctgttccgtg ggttgtgatc tccccttttg ccatatccag
    10081 ttttgctatg gcattatttt cattacttcc tgtatcggta gtactttctt catcaagaga
    10141 agatacacac gcacgcgcgc ttccgggaga tgcacacgca agaacgccga ctgcatgatt
    10201 acccgcagta acaataaatg tatcatctcc gtcagaaaaa gagggggtaa ccgaaccgtc
    10261 agagttgact cttattatgg tgtcagtatt caggtttcca ctcgtttgac ttagcaaacc
    10321 aattgagtaa tcccccgtta tgacaatcgt gtttttaccc tgcaaaataa cagcaccgcc
    10381 aaattcactt acgattgcgc tagctgttaa tgctgtgctt agaggactat tgtcagaagg
    10441 atataaaccg cccgttgtta caatgacgcc attgttaatg gttaacttgc taccctctcc
    10501 tgcaacggat acccgtaact tccattaccc gttgttataa ctagcgcatt atcaatatct
    10561 tttacttttc catcttttat tgccacagca tgtttatatt catcgtgctg aataaactgt
    10621 tcagcatatg cattacccac cgccagagca ataagtccca aactaaaccc ggagagtttt
    10681 ttcagtttca tagattaacc atccattcat tggattaaca aacattcctg gtcaaataac
    10741 ctatgcgttc aga
//
LOCUS       DAZGJW010000153        10573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZGJW010000153 DAZGJW010000000
VERSION     DAZGJW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.153"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(97..172)
                     /locus_tag="ACHKIK_004110"
     tRNA            complement(97..172)
                     /locus_tag="ACHKIK_004110"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(137..139),aa:Lys,seq:ttt)
     gene            complement(319..394)
                     /locus_tag="ACHKIK_004111"
     tRNA            complement(319..394)
                     /locus_tag="ACHKIK_004111"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(359..361),aa:Lys,seq:ttt)
     gene            complement(398..473)
                     /locus_tag="ACHKIK_004112"
     tRNA            complement(398..473)
                     /locus_tag="ACHKIK_004112"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(438..440),aa:Val,seq:tac)
     gene            complement(623..698)
                     /locus_tag="ACHKIK_004113"
     tRNA            complement(623..698)
                     /locus_tag="ACHKIK_004113"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(663..665),aa:Lys,seq:ttt)
     gene            complement(701..776)
                     /locus_tag="ACHKIK_004114"
     tRNA            complement(701..776)
                     /locus_tag="ACHKIK_004114"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(741..743),aa:Val,seq:tac)
     gene            complement(912..987)
                     /locus_tag="ACHKIK_004115"
     tRNA            complement(912..987)
                     /locus_tag="ACHKIK_004115"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(952..954),aa:Lys,seq:ttt)
     gene            complement(1152..1943)
                     /gene="cpoB"
                     /locus_tag="ACHKIK_004116"
     CDS             complement(1152..1943)
                     /gene="cpoB"
                     /locus_tag="ACHKIK_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO9085178.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1953..2474)
                     /gene="pal"
                     /locus_tag="ACHKIK_004117"
     CDS             complement(1953..2474)
                     /gene="pal"
                     /locus_tag="ACHKIK_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO9085179.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2509..3801)
                     /gene="tolB"
                     /locus_tag="ACHKIK_004118"
     CDS             complement(2509..3801)
                     /gene="tolB"
                     /locus_tag="ACHKIK_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO9085180.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3934..5199)
                     /gene="tolA"
                     /locus_tag="ACHKIK_004119"
     CDS             complement(3934..5199)
                     /gene="tolA"
                     /locus_tag="ACHKIK_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO9085181.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(5264..5692)
                     /gene="tolR"
                     /locus_tag="ACHKIK_004120"
     CDS             complement(5264..5692)
                     /gene="tolR"
                     /locus_tag="ACHKIK_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO9085182.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5696..6388)
                     /gene="tolQ"
                     /locus_tag="ACHKIK_004121"
     CDS             complement(5696..6388)
                     /gene="tolQ"
                     /locus_tag="ACHKIK_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO9085183.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(6385..6789)
                     /gene="ybgC"
                     /locus_tag="ACHKIK_004122"
     CDS             complement(6385..6789)
                     /gene="ybgC"
                     /locus_tag="ACHKIK_004122"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO9085184.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6939..7232)
                     /gene="ybgE"
                     /locus_tag="ACHKIK_004123"
     CDS             complement(6939..7232)
                     /gene="ybgE"
                     /locus_tag="ACHKIK_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO9085185.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(7232..7345)
                     /gene="cydX"
                     /locus_tag="ACHKIK_004124"
     CDS             complement(7232..7345)
                     /gene="cydX"
                     /locus_tag="ACHKIK_004124"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO9085186.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(7360..8499)
                     /gene="cydB"
                     /locus_tag="ACHKIK_004125"
     CDS             complement(7360..8499)
                     /gene="cydB"
                     /locus_tag="ACHKIK_004125"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO9085187.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8515..10083)
                     /gene="cydA"
                     /locus_tag="ACHKIK_004126"
     CDS             complement(8515..10083)
                     /gene="cydA"
                     /locus_tag="ACHKIK_004126"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO9085188.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2613 a         2846 c         2513 g         2601 t
ORIGIN      
        1 aggattcgaa cctgcgacca attgattaaa agtcaactgc tctaccaact gagctaacga
       61 ccccttgcgg gattatgctg ctttaatcat tcaggatggt gggtcgtgca ggattcgaac
      121 ctgcgaccaa ttgattaaaa gtcaactgct ctaccaactg agctaacgac ccatacgggt
      181 gttacctgaa agatttctac tcggggccaa tctttaagat tggtgtgtcg tgcaggatga
      241 ctcgcttcgc ttgccctacg ggccgttgct ggcgcaacgt tatccttcac gcttaacatc
      301 tgagtttgat gttaaaattg gtgggtcgtg caggattcga acctgcgacc aattgattaa
      361 aagtcaactg ctctaccaac tgagctaacg acccgagtgg tgggtgatga cgggatcgaa
      421 ccgccgaccc cctccttgta agggaggtgc tctcccagct gagctaatca cccgatactg
      481 cgctggatac tacaaactac ttccaccgaa cccaccgtaa ttagtggtgg gtcgtgcagg
      541 atgactcgct tcgctcgccc tacgggccgt tgctgacgca acgttatcct acacgcttaa
      601 catctgtctt cgatgctaaa actggtgggt cgtgcaggat tcgaacctgc gaccaattga
      661 ttaaaagtca actgctctac caactgagct aacgacccgg tggtgggtga tgacgggatc
      721 gaaccgccga ccccctcctt gtaagggaga tgctctccca gctgagctaa tcacccaata
      781 ctacgctgga tactacaact aactatgtta gtggtgggtc gtgcaggatg actcgacttc
      841 gtctcgccct tcgggccgtt gctgatgcaa cgttatcctt cacgttttac taccgcttcc
      901 accttagcga ttggtgggtc gtgcaggatt cgaacctgcg accaattgat taaaagtcaa
      961 ctgctctacc aactgagcta acgacccact ttttcgttgc tttcggtttg tttgatatcc
     1021 cgtggcaacg gcggcatata ttactgattt cagacttgag cgcaacaaaa atttcgatgc
     1081 agatcactca actgcttatg attcgcacga cacgaccaga aataatgcga cttctggtcg
     1141 tgtgttatgc attacatcgc gttcagacgt ttttgtgcct gtttagcgcc atcagtacca
     1201 gggtatttac tgataacctg ctggtatacg gctttcgctt ttgcggtgtc acctttgtcc
     1261 tgcatgatga cgccgacttt aaacatcgca tcagcagcct ttggtgactt cggatagttt
     1321 ttcactaccg aagcaaaata gtacgccgca tcatcttttt tacccttgtt gtagtttaac
     1381 tgacccagcc aataattggc gtttggcagg taagttgaat cagggtaatt tttgatgaaa
     1441 ttctgaaatg ccaccattgc gtcatcctgg cgggatttat cctgcaccag cgcaatagct
     1501 gcattgtaat ccgtgtttgc atcgccgctt ttcaccggcg cgccagcatt cgcagtacca
     1561 gcatcagctg tcggcgtcgt tgatgccgcc gcaccgcttt gatcgctgct ggttgattgc
     1621 gccgctgcac caccgctgct gaggctgtcg atctgcaaca ggatctgctt ttgccgctcc
     1681 acgacctgat tcagttgata ctgattttcc tgaatctgac cacgcaggga atcaatatcg
     1741 gattgattat cagaaagttg ttgctggagt tgggttaaaa gctggctgtg agcattagaa
     1801 atacgctcaa gttgagtgac gcggtcttcg accgagcctg agccgacact actgattggt
     1861 gcctgagcaa aagcggccca gggggccgct ataccaacca gtaacgacag actcaatagt
     1921 tgatgtctga agttactgct catgcaattc tcttagtaaa ccagtaccgc acgacggttt
     1981 ttggagtatg ccgcttcgtc atgacccagt actgcaggtt tttctttacc gtaagaaacg
     2041 atggagatct ggtctgcaga aacgccttta ccctgcaggt acatcttaac ggcgttcgca
     2101 cgacgttcac ccagggagat gttgtattcc ggagtaccac gttcgtccgc gtgaccttct
     2161 acggtgactt tgtaagacgg gttgctacgc aggaagtttg catgtgcatc cagcatttga
     2221 gcgaagtcag aacggatatc gtacttgtcc agatcgaagt aaacgatgtt gttctgctgc
     2281 agctgttgca tttgcagacg agcctgctct tcggaagaca tgttgccgtt gccgccgttc
     2341 gcatccatac cagtgccggc acccagcatg ccttcgctgc cgtcattgct ggcgttcttg
     2401 ttggaagaac atgccgcaat tgccataaca ggcagagcaa tcatcagccc tttcagcact
     2461 ttgttcagtt gcatttcaat gattccttta ctattcaatt aattattatc acagatacgg
     2521 cgaccaggca gggaatttga cctgtccatc agttgccgga agacgcgctt tgaaacgccc
     2581 atctgtagaa accaaattca gcacggatcc catcccctga gaagagctgt agattaccat
     2641 agtgccgtta ggtgccagac ttggcgtttc atccaggaac gtggacgaca gaacttgtac
     2701 gcctcccgtt gccagatctt gtttggcaat gtgctgctgc ccaccattgg agctgaccat
     2761 taccataaat ttaccgtcgc tgctgacatc cgcatcctgg ttctgcgaac cttcccaggt
     2821 aatacgttgt ggcgcaccgc cgttgatatt cactttataa acctgcggac gaccggcctg
     2881 gtcagaagta aatgccaggt tctggctatc cgggaaccag gtcggttcgg tattgttact
     2941 gcgaccatca gtcacctggc ggatctgacc agaagccaaa tccattacgt acaggttcag
     3001 actaccggtt ttcgacaagg cgaatgccag tttgctgccg tctggcgaga atgcaggtgc
     3061 accgttgtga cgcgggaatg aagccacctg acgtacagcg ccatttgcca gcgtctgaat
     3121 aaccagcgcg gaacgaccgc tttcgaaggt cacataagcc agtttagaac cgtctggtga
     3181 ccacgccgga gacatcagcg gctgcggtga acggtgaacg acaaactggt tgtaaccgtc
     3241 atagtcagat acgcgcagtt catacgggaa ctgaccgccg ttggtctgaa caacgtaggc
     3301 aatacgagta cggaacgcac ctttaatgcc ggtcagtttt tcaaacactt catcactggc
     3361 ggtatgacca gcataacgca gccactgctt gttcactttg tacgagttct gagcaagtac
     3421 agtacccggt gcgccgccag tgtcaacaag ttgataagca acattgtaag aaccatccgg
     3481 attcggagtg acctgaccga caactacagc gtcaatgccc agtgcggacc atgcagctgg
     3541 ttgtacttcc tgcgcactac ccggctgctg tggcagacga gcgcgatcta acggattaaa
     3601 tttaccgctg ttacgcaagt cagcagcaac gatgccgcca atatcttcag gtgccgcacc
     3661 aggccccgcc cactggaaag gaacaacacc aataggacga ccggaatcta caccgctgtc
     3721 gatcacaatg cggacttcag catgcagaac tgatgcccac agtatgagaa aaccaaatgc
     3781 tactcgtaat gcctgcttca tcatatctcc cttatctgga ccgatggccc acgataattt
     3841 agcagaatgt taacaaacta aaatacacaa aactaccaga accccgtggc aaccggtgcc
     3901 tgatgttgac cgtccgaaca gtcaacatcg cgattacggt ttgaagtcca atggcgcgtt
     3961 tttgaacact tcatatactg cctggcttgg tggtttcggg atcttcgcaa gtttagctgc
     4021 tgccaacgca gcctgacaaa gtgcgggatc gccaccttca ggtttgatat ccagtaacat
     4081 accatcgggt gccagtttta tgcgcagcgt acaggttttg cctgcatagg acgatgcgtc
     4141 atagaactta ctttcgatag cagatttaat ctgcccggca tagttattga tatcggcccc
     4201 tgatgcgcca ttgtttttag tattaccact cccggcaggc gaagcattgt tccctttcgc
     4261 ccctcccccc gttttcggtg cattcttacc agagcttagc tcaccgaaaa tatcatctgc
     4321 ctctgcggcc gcttttgctg cagcggcttt ttcagctgca gcttttgccg ctgccgcttt
     4381 tttgtctgcg gcggcttttt ctgccgctgc tttcttatca gctgctgcct tttcagcagc
     4441 cgctttttct gctgcttttt tgtcggctgc agctttctct gctgccgctt tcttatcagc
     4501 tgcagccttt tcagcagccg ctttcttctc agcttctgct ttggcttttt cagcagcttc
     4561 agttgccgct ttctttcttg cttcagctgc agctgcttct gccgcttccg ctttcttctt
     4621 cagtgccgca gcggctgcct cggctttttt ctgcgcttcg gctgcggctt tggcggcttc
     4681 tgcttctgct tttttctttg cgtctgcagc cgctttcttc gctgcttctt ccgcagcttt
     4741 agcatctgct tcggccttcg ctttagcatc tgccgccgct ttcgctgccg cctcttcagc
     4801 ttgcttctgc tttaactcgg cctgttttgc ggcttcttca gcctgctttt tctgctcctg
     4861 agccgctaac cgctctttct caagttgctt caggcgttcc tgttcagccg cttgtttctc
     4921 acgcagttct tcagcagcct gctgttcctt catcttgcgc tgttcatcag aacgcttcgc
     4981 gcttgattcc tggctttgca tgcgtttgta ctgctcaact accgcacctg aatcaaccat
     5041 gacagcgtcg atggacgaac caccgccgcc tccggctgaa gcttctatat tctcatcgaa
     5101 cgaactccag atcagcgccg caaataagat gacatgcagc actgctgaaa taattatcgc
     5161 ccgcttgagc ttgtcgtttt gttcggttgc ctttgacact ctcggtttcc aaaaactgtt
     5221 cgcctgttac ccgctctctt tcaagcaagg gaaacgcaga tgtttagata ggctgcgtca
     5281 ttaaaccaac cgatttcaca cccgcactat gtaacaagtt cagtgcttta attatttcat
     5341 cgtaaggcac atcttttgcg ccaccgatca gaaagaccgt tttcgggttg gccttgaaac
     5401 ggctggacac ttccgccacc acctgctctg gtggtaaacg ctccaggcga tctttctcaa
     5461 ccaccacggt gtactgacca ataccagaca cttcaacaat cactggcgga ttatcgttac
     5521 tgctcaccgc ctgtgattca gtagcgtctg gcagatcgac ctccacgctc tgggtgatga
     5581 tgggcgctgt cgccatgaag atcagcaaca gcaccagcag tacgtccagc aacggtacaa
     5641 tgttgatttc ggacttaagt tcgcgacgac ctcgtccacg cgctctggcc atgacttacc
     5701 ccttattgct ctcgctaacg gtaaacgcct ggcggtgcag aatcgcggta aactcttcca
     5761 taaagttgtc gtaattcagt tccagtttgt ttacgcgctg gttgaggcgg ttgtaggcca
     5821 taactgccgg gatagcggca aacagaccaa ttgcagtcgc aatcaacgct tctgcgatac
     5881 cgggcgcaac catttgcagt gttgcttgtt ttaccgcccc gagggcgata aaggcgtgca
     5941 tgatccccca gaccgtacca aacagaccaa tatacgggct gatggagcca accgtaccga
     6001 ggaacggaat gtgcgtttcc agattttcaa gttcacggtt catggagata cgcatagcac
     6061 gcgacgcccc ttccactacg gcttccggcg catggctatt ggcacgatgc aggcgcacaa
     6121 actctttgaa cccgctgtaa aatatttgtt ccgaaccagt cagattatcc cgtttcccct
     6181 gactctcttg atagaggcga gagagttcga ttccagacca gaatttatct tcaaacgctt
     6241 cggcttcgcg cgccgctgcg ttaagaatac gggtccgctg gataataatg gcccaggatg
     6301 cgattgaaaa accaatcaaa atcaacatga taagtttaac cagaaggcta gccttcagga
     6361 acaaatcaag gatattcatg tcagtcactg cttaaactcc gcgacaatag acttgggaag
     6421 cgcacgaggc ttcattttga gtgggtcaac gcaaacaacc agaacctctg cttcattcag
     6481 caaagtattc tcggcgttga caatacgttg cgtgaaaacc aaagaggtgc cacgcattga
     6541 tgttatttca gtctgtattt cgagcatatc gtcgagccgc gcaggtgcgt aatattccac
     6601 cgtcatttta cgtaccacaa aggcaacgcg ttcagccatc agcgcctgct gactgaagtg
     6661 atgatgacgc agcatctctg tgcgtgctct ttcataaaaa gcgacgtaac tggcgtggta
     6721 caccacacca ccggcatcgg tatcttcata gtagacgcga accggccatc gaaacagcgt
     6781 tgtattcact ttacatcccg gtaatgcaac aaaagttaga gcttttaaac gctgctacta
     6841 tacgcgcgcg attgaggttt gggaatgagg gcaagttaag ggagcgtaaa aatttatggg
     6901 cctggagcgg cccataattt tttaaagaga attctgattt aaaagaagaa gaaaatcagc
     6961 ccgacaatga gaacaatatc ggcaagcaat gggcaaaaaa tcccttgcca aagaaccttc
     7021 tgcggacgaa agcccacgcc gtgaatcaca ccagcacaga cggcccacat cagcaataaa
     7081 ccatgccaga tttccagttc actggtcttc gcggcaaaac gagatgggtc ccaaaacata
     7141 catcctgcta acagaagcgc catcacgaag gaaagcgccc gcagggggcg cttgtccatt
     7201 accgcataca aagtcgcgat aatcttactc atcagatgtc ttcttgaccg gctttgcctg
     7261 attcgacgtg ttcaagcgcc agcgcggtga ttaccccaaa cgaacaggca agaagcgttc
     7321 ccagaatcca tgcgaaatac cacattttta gctccttact tagtacagag agtgggtgtt
     7381 acgttcaata tcttctttgg tgatacgacc gaacattttc cagtaacacc aggcggtgta
     7441 gagcagaatg atcggtacca gaaccaccgc aacccaggtc atgacgttaa gcgtcagctg
     7501 gctggaagtt gcatcccaca ttgtcagact tgcgttcatc atggtgctgg acggcatcac
     7561 aaacgggaac attgcgatac cggctgtcag gatgatgcag gccagcgtca gggaggagaa
     7621 cacaaacgcc cacgcggctt tatccatacg tgcagtcagg atggtcagca gcggcagaac
     7681 cacacccagt gccggaatag cccacagaat tggcgtgttg ttgaagttaa ccagccatgc
     7741 gccagcttca cgaaccactt ctttattcag tgggttagag gctgcgtaat ggtccattgt
     7801 cgatttcacg acataaccat cgataccgta catcacccat acgccagcca gtgcgaaaca
     7861 gaccagtgtc accagcgcag ccacctgagc cgttgcacgg gtacgcaggt gcagttcgcc
     7921 cacggtacgc atttgcagat aggttgcgcc ctgagtaatg atcatcccta cgctcaccac
     7981 gcctgccagc aggccgaacg ggttaagcaa ctggaagaag ttaccggtgt agtacagacg
     8041 cagatattca tcaacgttga acggtacgcc ctgcaacagg ttaccgaacg ctacaccaat
     8101 taccagcggc ggaacgaagc taccaatgaa gatgccccag tcccacatgt tacgccagcg
     8161 ggtttcttca atcttggagc ggtagtcaaa accgaccgga cggaagaaca aagacgccag
     8221 cacgaggatc atcgccacat agaagccgga gaacgcagcg gcatagacca tcggccaggc
     8281 agcaaagagt gcgccgcccg cggtgatcag ccaaacctgg ttaccgtccc agtgtggtgc
     8341 aatggagtta atcataattc gacgctcggt gtcgttacga ccgaggaaac gggtgagcat
     8401 gcccaccccc atgtcgaaac cgtcagtgac tgcaaaacca atcagcagaa cgccaaccag
     8461 cagccaccag ataaaacgca atacttcata atcgatcatt tgacgactcc tgtcttagcg
     8521 tgccggctga gtagtcgtgg aagactgctc aaagtgatag cgaccggttt tcaggctgct
     8581 tgggccgagg cgtgcaaatt tgaacattaa gaacaattct gccaccagga acagggtata
     8641 caggccgcaa atcagcacca ttgagaagat gagatcgcct gcggtcagtg acgagttcgc
     8701 cacagctgtc ggcagcactt caccgatagc ccacggttgg cggccatatt cagccacgaa
     8761 ccagcccgct tctacagcaa tccacggcag cggaataccg tacagcgcgg cgcgcagaag
     8821 ccattttttc tcgccaatgc ggttgcggat gacactccag aaagagagcg cgatgattgc
     8881 cagaagcagg aagccacacg ccaccatgat acggaacgca aagtacagcg gcgctacacg
     8941 cgggatggag tctttggttg cctgttgaat ctgcgcttca gtcgcatcag ccacgtttgg
     9001 cgtatagcgt ttcagcagca gaccgtaacc gaggtctttc ttcatgctat tgaactggtc
     9061 acgaaccgcc tggtcggtag aaccagaacg cagttgttcg agcagagagt acgccttcat
     9121 cccgttacga atgcgttctt catgctgcac catcagctct ttcaggccga taaccggggt
     9181 atccacggaa cgcgttgcaa tgatgcccag tgcgtaaggg atctgaatcg caaatttgtt
     9241 cgtctcctct tcctgatcag gaatgccgaa cagagtaaag gcagcaggcg caggttgcgt
     9301 ttcccactcg gcttcaatag cagccagttt ggttttctgc acgtcgccca tttcgtagcc
     9361 ggattcatca cccagaacaa taacagacag aacagcagcc ataccgaagc tggcagcgat
     9421 agcaaaggag cgtttagcga aggcgaagtc acgacctttc agcatatacc atgcgctgat
     9481 accgaggatg aacatcgcgc cagtcacata accagacgct acagtgtgaa cgaatttcac
     9541 ctgagcaacc gggttaagca ccagctcgga gaagctcacc atctccatac gcatagtttc
     9601 aaagttgaaa tcggacgcga ttgggttttg catccagccg ttcgcaacca gaatccacag
     9661 tgcggacagg tttgaaccga gcgccaccag ccaggtgaca cacatatgct gaactttacc
     9721 cagacgatcc caaccgaaga agaacagacc tacaaaggtg gattcgagga agaaggccat
     9781 cagaccttcg attgccagcg gcgcaccgaa gatatcccct acatagtggg aatagtaaga
     9841 ccagttagtc ccgaactgga actccatggt cagaccggta gccacaccca gagcgaagtt
     9901 gataccaaac aacttgcccc agaacttggt catatcttta taaatctgtt tgccggagag
     9961 gacgtagacc gtttccataa tggccagcag gaacgccata ccgagcgtca gtggcacaaa
    10021 aaggaagtgg tacatcgcgg tcaaggcaaa ctgtaagcgc gacagttcga ctatatctaa
    10081 catcatgact ccttgctcat cgcatgaaga ctccgagagt gaaccccgtt agaaagggtc
    10141 acacgcatgc cccaatagaa atttgtttga acactttcat cataccatgt gaatgcacac
    10201 aaaaacggcg taaagaaagg ttgcaaacat gttaataaaa actcaaattg atcccacgta
    10261 tatattacgc cgcaaaatcc ttacaataaa caggtttttg ttgaagcaaa tttgcatttt
    10321 tcgacggtga tcaatttata gctaaattac cgcctttcag ccaatttgat cgagaacaat
    10381 ttatctcttt ttgatgccca tttccaagac ttatacattg ataaatatca attcctacaa
    10441 attatgttaa tcatgtttca ataaggtaac tttagtgaca tttatgttta aaatgtgtga
    10501 gttataaggc tgaaattagt ttcggttggc aattaaagat aaaaagagac gaagcaaaaa
    10561 cagcacggtt gac
//
LOCUS       DAZGJW010000154        10465 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZGJW010000154 DAZGJW010000000
VERSION     DAZGJW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.154"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(500..874)
                     /locus_tag="ACHKIK_004127"
     CDS             complement(500..874)
                     /locus_tag="ACHKIK_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085189.1"
                     /translation="MEFKDLPMPFQEMAANVVRSQLATLDLSTVEKETIDNISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLELMDIINKGFGLLMKKKGIRIAPLENHFTASSINS
                     CDLKHHTSDGKVESNNKISINH"
     gene            complement(897..1115)
                     /gene="ydfC"
                     /locus_tag="ACHKIK_004128"
     CDS             complement(897..1115)
                     /gene="ydfC"
                     /locus_tag="ACHKIK_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO9085190.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITRLEKAAALSIARMITYPR"
     gene            complement(1145..1273)
                     /gene="ydfB"
                     /locus_tag="ACHKIK_004129"
     CDS             complement(1145..1273)
                     /gene="ydfB"
                     /locus_tag="ACHKIK_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO9085191.1"
                     /translation="MDFNTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSNG"
     gene            complement(1275..1430)
                     /gene="ydfA"
                     /locus_tag="ACHKIK_004130"
     CDS             complement(1275..1430)
                     /gene="ydfA"
                     /locus_tag="ACHKIK_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO9085192.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(1623..2030)
                     /locus_tag="ACHKIK_004131"
     CDS             complement(1623..2030)
                     /locus_tag="ACHKIK_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9085193.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            2108..2335
                     /locus_tag="ACHKIK_004132"
     CDS             2108..2335
                     /locus_tag="ACHKIK_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO9085194.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELLYDPKVYDEYRKTKRAGRLNNENHS"
     gene            2319..2840
                     /locus_tag="ACHKIK_004133"
     CDS             2319..2840
                     /locus_tag="ACHKIK_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO9085195.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            2821..3786
                     /locus_tag="ACHKIK_004134"
     CDS             2821..3786
                     /locus_tag="ACHKIK_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085196.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLSESIGLKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDQQTAEQDFLTRNPDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVNLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDQFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            3827..4246
                     /locus_tag="ACHKIK_004135"
     CDS             3827..4246
                     /locus_tag="ACHKIK_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO9085197.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYSSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKARRSRLLLTPSCNVPFEPF"
     gene            4301..5398
                     /locus_tag="ACHKIK_004136"
                     /pseudo
     CDS             4301..5398
                     /locus_tag="ACHKIK_004136"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1202-like element ISEsa1 family transposase"
     gene            5471..5620
                     /gene="ydfW"
                     /locus_tag="ACHKIK_004137"
     CDS             5471..5620
                     /gene="ydfW"
                     /locus_tag="ACHKIK_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfW"
                     /protein_id="HFO9085198.1"
                     /translation="MDNARIDLRSKYYVKSKADHPWLTRRTQSHQQVKPPKLPKKKPD
                     PDKID"
     gene            complement(6057..6389)
                     /gene="flxA"
                     /locus_tag="ACHKIK_004138"
     CDS             complement(6057..6389)
                     /gene="flxA"
                     /locus_tag="ACHKIK_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFO9085199.1"
                     /translation="MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISS
                     EEGMTTQQKKEMAALVQKQIESLRAQLEQLLRQQAEKKNKDATVQPDKKEEKKDDTNT
                     AGTIDIYV"
     gene            complement(6592..6897)
                     /gene="ydfV"
                     /locus_tag="ACHKIK_004139"
     CDS             complement(6592..6897)
                     /gene="ydfV"
                     /locus_tag="ACHKIK_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFO9085200.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLVFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSVLLSRRRVSGDLTCR"
     gene            6922..7161
                     /gene="relB"
                     /locus_tag="ACHKIK_004140"
     CDS             6922..7161
                     /gene="relB"
                     /locus_tag="ACHKIK_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFO9085201.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            7161..7448
                     /gene="relE"
                     /locus_tag="ACHKIK_004141"
     CDS             7161..7448
                     /gene="relE"
                     /locus_tag="ACHKIK_004141"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFO9085202.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            7520..7675
                     /gene="hokD"
                     /locus_tag="ACHKIK_004142"
     CDS             7520..7675
                     /gene="hokD"
                     /locus_tag="ACHKIK_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO9085203.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            7892..8143
                     /gene="rem"
                     /locus_tag="ACHKIK_004143"
     CDS             7892..8143
                     /gene="rem"
                     /locus_tag="ACHKIK_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFO9085204.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFSGE"
     gene            8210..8488
                     /locus_tag="ACHKIK_004144"
     CDS             8210..8488
                     /locus_tag="ACHKIK_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085205.1"
                     /translation="MARNAKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            8490..9539
                     /gene="ydfU"
                     /locus_tag="ACHKIK_004145"
     CDS             8490..9539
                     /gene="ydfU"
                     /locus_tag="ACHKIK_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9085206.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKSPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEDKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            9553..10305
                     /gene="ydfT"
                     /locus_tag="ACHKIK_004146"
     CDS             9553..10305
                     /gene="ydfT"
                     /locus_tag="ACHKIK_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO9085207.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
BASE COUNT         2772 a         2281 c         2655 g         2757 t
ORIGIN      
        1 gtgcctggtg cctccaggtg acgttaacca gttaacaatt aacgccggat aaagagaatc
       61 cacccataac actgtttttg gttttaactg ttccgcgtgc gctcagccgc attcaccaca
      121 tcacaaaatt cactttaaaa agggcggcag agcagtcacg gagtaaaact gataccgcca
      181 aaagtcacca gaaaattgat aacagagggc gttgcagcgg agttgtcact taagcgtatg
      241 gtcaacctga caacccggtg tcctcaacgg gggaaggaat aaccccgcca tacttaccgc
      301 cgcgccattt cgcggattgc cacaaccgga agcgcacgat cgaattaaat ttaacgacga
      361 cctatacaga gagactaact tctccgggcg ctttcgtgtt atgccctgac ttttcaggga
      421 tatatccttt tcagtaaact gtcagtgccg gattcttatc cgtgtccggc gcacgcactc
      481 tacctcacct gtgaataaat taatgattaa ttgatatttt gttgtttgat tcaactttcc
      541 catcggatgt gtgatgcttt aaatcacagg aattaatact gcttgctgta aaatgatttt
      601 caaggggagc tattcgaatc cctttctttt tcattaacaa gccaaatcct ttattaatga
      661 tgtccattaa ttccagaaag tatttttcat gtaaatcctg gttatcagag agctgcttct
      721 cttcgtacag cccgataaag gctcggcgta cgttaccaga tatattgtcg atggtttctt
      781 tttctacggt actcaggtca agagtcgcca gttgagagcg aaccacattc gctgccattt
      841 cctggaatgg cattggtaaa tctttaaatt ccattattag cctcgttggt tagctattaa
      901 cgcgggtatg taatcattct ggcaatgctt aatgccgctg ctttttccag cctggtgata
      961 tcctgctcca gagcggacag attttcagcc tgcttagccc tggcttcatt ggcccatttc
     1021 aggtcctgcg cagccttaat tttctggtgc atccactcat aaagttcatc atcggtatag
     1081 tctggcgcga tgatgacggg ttctcgtttc tgcatactga ttcctcgcgg tgctgtttcg
     1141 cttatcagcc gttagatttt gccgaactgg aaagcgcctg tttaaactca ctgaagctga
     1201 gggcttcttc gccttcggca aggccttcga agtattcttc gtaagccttt tccatgattg
     1261 tgttgaaatc catatcactc acctgagttt ctttccagcc agcgacgggc accattttcg
     1321 gttttaaacg ttttgctttt ggtatacgtc atcgcggtga acgtgccgtc ctggttgggg
     1381 aacacgccac ataccagaga ttcgctgttg ccaagatcga tagtatccat gctgacctca
     1441 tttcccctta acgccggggt agcggaactg tttgctgaga acaccgtgcg gtgtcttgat
     1501 ggaggtaatt tagttttctc attatttttc gtcaaggttt tttgatgaga aaactcaagc
     1561 gttggcgcaa gataaagcca atacattgaa atgtaaggct ttaaaatttt gtgaagggat
     1621 gattattgat gcttgttgcg tttgcgagcc tctaatagct cggtgaacag gcgattaaaa
     1681 ttctcaacgc gggcgcggag ttcgctgagt tgtgcttgtt gctctgattt aggaagtgcg
     1741 cgatacaaac gcagcatctc taactcatct tccgataagt ctaaggcact atcgagtgaa
     1801 acagggggag ctggtgtttt gtcctcgtcg ccaaacagta tccaagttgg tgaacattgc
     1861 aatacctcgg cgaggcgatg caaattttgc ccgcgcgggg ctgtatggtc actttcccat
     1921 aatgaaattg atgagccaga tacgccagca gctttgctta aaccgttttg acttaagcct
     1981 acctgcttac gtctttctct aatgcgttga cctaaagttt tctcgttcat atttagatat
     2041 cttaataacc cttgacttga gattccttga gtgattagca ttgagaaaac tcaatattgg
     2101 aggtgcgatg tttaaatcag acgtaattaa tttttacggt acgaaagcca aagtagcgaa
     2161 agctgctggc gttgacccat ctgctgtttc tcaatggcaa gagctggttc ctgaaggtcg
     2221 cgcgatgcgt ctacaggagg catctggcgg cgagcttctg tatgatccca aggtttatga
     2281 cgaatatcgt aagacgaagc gggcggggcg gttgaacaat gaaaatcact cctgaacagg
     2341 ctcgtgaggc tctggatgcc tggatatgtc gaccaggaat gacacaggag caggcgacga
     2401 tattaatcac tgaagcattc tgggctttga aagagcgccc gaacatcgat gttcagcgtg
     2461 tcacagatga aggtggcgcg gttgatcagc gagcacttgg cgttaatcga gtgaagatat
     2521 tcgaacgctg gaaggctatc gacaccaggg ataagcgtga aaagttcacg gcgctagtgc
     2581 ctgcaattat ggaggctatt cggattaatg atttcaggtt gtatcgtgaa attagtgacg
     2641 gaaaaagcat cacgtacatg atcgccgggt taaacaaaga atatggcgat gtggtggagt
     2701 ccggactgct ttttgctgat cctgccgtag tggatcgtga aactgacgaa cttatagaaa
     2761 aagcaattgc tttcaaactt gcgtatcgac agcaatacca acaaaaagct ggatggaatt
     2821 atgagccttc tttttgctga acgcccactg gttataaaca cacagcttgc gatgaagatt
     2881 ggcttaaatg aagccattgt gttgcagcag ttgcattact ggttgagaga taccaattcc
     2941 ggcatggaat gtgatggtgt tcgctggatt tacaacacaa cggaacaatg gctggaacag
     3001 ttcccattct ggtcagagtc aacgttaaag cgcgcgtttg caagtctgaa aacgctgggg
     3061 cttttgcgtt gtgaaaagct caataaatca aagcgcgata tgaccaattt ctacacgatc
     3121 aactacggga gcgagctttt agatggtggc aaattgagcg aatccatcgg tttaaaatgc
     3181 gccgctccat caggtcaaaa tgacacgatg gaagaggtca aaatgaaacg ctccattggt
     3241 tcaaaacgac ccaatgtcat cgggtcaaaa tggcctgatg atcttacaga gaatacaaca
     3301 gagattacta cagagaataa aaacactttt cgtccggaag cttcgcaacc ggaccagcag
     3361 accgctgaac aggatttttt aacccggaac cccgacgcgg ttgtgtttag tgcgaaaaaa
     3421 cgccagtggg gtagcaggga ggatttggcg tgtgcgcagt ggatttgggg gcggatcgtg
     3481 aacctttacg aacaggctgc cagcgacgat ggagagatca tgcgaccaaa agagcctaac
     3541 tggacagcct gggccaatga cgtgcgcaca atgcggatgc tggatggcag aagccacaga
     3601 caaatttgcg aaatgtttgg tcgggtacag cgagatcaat tctgggtaaa aaacatcatg
     3661 agcccgtcaa aactccgcga aaaatgggac gaactggtca tccgcctggg acgttcacct
     3721 gtacagcgtt gtgtgaatca tatttctgaa ccggatacag aaattccgcc tggtttcagg
     3781 ggataagtgt tgattttagg gtataaagta atgttcagga ggacttgtgg caaaagtttt
     3841 tacacaagaa gagcgggaaa aaattaaagg gcaggtggtg gaactcgtac gccagagtgg
     3901 gcgcgagacg ttacgacaac tggaagctaa aactggggca acaagatatc tgatgagcgt
     3961 tctggccaga gagctggttg ccagtggcga tgtatacagc tctggttacg ggttattccc
     4021 gtctgaacag gcgcgtaagg actggcaaaa cgcccgcaaa aaactctcga gggcaaaggt
     4081 gaagaaacct gctgtggttg atccggacct tatctggtca ttacctgacg gagaaatacg
     4141 tcgctacgac agtcgtctaa acataatctg tcgcgagtgc cggaaggcaa ggagaagcag
     4201 gctattgtta acaccaagct gtaatgtccc ctttgaacca ttctaaaatg tccccagaca
     4261 attctctggg ggatttttca tgatcaaaga gactgttacg atgagtcata aggaactcca
     4321 ccgacttcag attattcagg agtcaattaa tcgacatatt actcaggaac aagctgcggc
     4381 acgcattggc atttctattc ggcaggttaa acgtctggtg caacggtata gaaatgaagg
     4441 gccttctggt ctggtttccc accgacgtgg aaagcgtcct aataattcct tttctactga
     4501 attcagagca acagtaattt cactcctcaa aggccgttac gctgattttg gacctacgtt
     4561 tgcgtgcgaa aaattgcgcg agatacacgg tttatcttta tccgttgaaa ctctcagaaa
     4621 gtggatgata gaagaggggt tatggcgtga acgccgtcgt aaaattgccc gtatatatca
     4681 acgccgcatg cgacgaccat cttacggtga actgatccag attgatggct cacctcatga
     4741 ctggtttgaa aatcgaggcc ccagatgtac actgatcgtt ttcattgatg atgccaccag
     4801 tgcgttgatg gcgttgcgtt ttgtgcctgc tgaaacaacc cgggcttaca tggaaaccct
     4861 ccggggttac cttaatgatc atggcgtacc gctcgctctc tactctgata gacacagtat
     4921 attcagggta aataacccag agcgggaagg tgagctgacc cagttcactc gtgcgataaa
     4981 gacactgggc atcgagccaa tccatgccaa cagcccgcag gcaaaagggc gggtagagcg
     5041 cgccaatcag acactacagg acaggctggt caaagaaatg cggcttcaga atatcagtga
     5101 tattgaaaca gcaaatgcat ggttgccgac ctttattgaa gcctataaca accggttcgc
     5161 tacgtcgcct cgtactactg ataatgctca tcttgatgtg caccattctg aagaggaact
     5221 gggttatatc ttcagcctac aggcgaagcg cgttctgtct aaaaatctca ctttccagta
     5281 caaaagcagt gcgtttcagg tacgcagtga gggccgggga tatcgactta ggcattcggt
     5341 tgttactgta tgtgagaact ttgacggtga aattaacgtt ctgtatgacg ggaaagcgct
     5401 gggctgggaa aagtatgttg atggcccgga gcctatacca ctggatgatg aaaagagtgt
     5461 ccatgaacga gtggataatg cccgtattga tttacgctca aaatactatg ttaaatctaa
     5521 agctgaccat ccctggctta cgcgccgaac gcaaagtcat cagcaagtta agcccccgaa
     5581 gttacctaaa aagaagcctg atcccgataa aatagattga aaccaagatc gattcggttg
     5641 agtgcatatc cattcatagg gtagattctt aagtcgcgtt tctgatgttc attttcgggt
     5701 ggtttgttac ttgttttacc ggggatatgc cagaaacgcg ctgagtcagt ctgggcggtg
     5761 cgcgtaatga ggcgttatgg taaatagcct atgctaatgt ccgctaagag caagaagcgg
     5821 aagttggcag ttttgtggac tgtccccaca aaagtgacta cagaaatagt tgcaattcat
     5881 aattgatcat gggttgtcag ttaaactcgt ggcgatttaa atagactaat tgggagtgcg
     5941 tccattactt atatcttgta atgttaacta tcagaaatga tacaaagata atatgtcttt
     6001 aaagaaaagg ctgatggcga aaagtggccc gatgagggcc acaatacggc tgtcacttag
     6061 acgtaaatat caatggtgcc agcggtattt gtatcgtctt ttttctcttc ttttttatca
     6121 ggctgaactg tcgcgtcttt attctttttc tctgcctgct gccttaacaa ctgctccagt
     6181 tgagccctga ggctttcaat ttgcttctgt accaatgcag ccatttcttt tttctgctgt
     6241 gtcgtcatcc cctcttccga tgagattttc ccaagctttt cagtcagcac ctgaatttgt
     6301 cttgtgattt tggctatttc tgatgttcct tccggggcgg agttgtttga aataacggtt
     6361 gaggtatttc cctgaattgt gacagacata gatttctcct tttaaaaaag cactatcggc
     6421 atgcacaaaa aaatctttaa tcgtatttct tgtgtcatta attgtttgat gttcagattg
     6481 ttttcctcgc gggctggcgc gcctcagaaa gtaaagcttg ttgacagggg taaacgttcg
     6541 gcaataattt tctgccgcat gcgggtgttg cataaaacgt gctacgttcc tttatcgaca
     6601 ggtcaggtca ccgctcaccc gccgacgaga aagcaacact gacatgctaa agcaaaaaat
     6661 agatgaataa gttgagttgt gcatatgtag cctgaccgtc acaaagtata tggtgtctgt
     6721 accagtaaga tgatggccgg actctttaaa aacgagctga cctgcacaat acaggatgga
     6781 cttagcaatg gctgctcctg gcacaaagcg gacagtgatc accgttctta cgactacttt
     6841 ctgacttcct tcgtgacttg ccctaagcat gttgtagtgc gatacttgta atgacatttg
     6901 taattacaag aggtgtaaga catgggtagc attaacctgc gtattgacga tgaacttaaa
     6961 gcgcgttctt acgccgcgct tgaaaaaatg ggtgtaactc cttctgaagc gcttcgtctc
     7021 atgctcgagt atatcgctga caatgaacgc ttgccgttca aacagacact cctgagtgat
     7081 gaagatgctg aacttgtgga gatagtgaaa gaacggcttc gtaatcctaa gccagtacgt
     7141 gtgacgctgg atgaactctg atggcgtatt ttctggattt tgacgagcgg gcactaaagg
     7201 aatggcgaaa gctgggctcg acggtacgtg aacagttgaa aaagaagctg gttgaagtac
     7261 ttgagtcacc ccggattgaa gcaaacaagc tccgtggtat gcctgattgt tacaagatta
     7321 agctccggtc ttcaggctat cgccttgtat accaggttat agacgagaaa gttgtcgttt
     7381 tcgtgatttc tgttgggaaa agagaacgct cggaagtata tagcgaggcg gtcaaacgca
     7441 ttctctgaac caaagcatga catctctgtt tcgcaccgaa ggtgacactt ctgctttgcg
     7501 ttgacaggag aagcaggcta tgaagcagca aaaggcgatg ttaatcgccc tgatcgtcat
     7561 ctgtttaacc gtcatagtga cggcactggt aacgaggaaa gacctctgcg aggtacgaat
     7621 ccgaaccggc cagacggagg tcgctgtctt cacagcttac gaacctgagg agtaagagac
     7681 ccggcggggg agaaatccct cgccacctct gatgtggcag gcatcctcaa cgcacccgca
     7741 cttaacccgc ttcggcgggt ttttgttttt attttcaacg cgtttgaagt tctggacggt
     7801 gccggaatag aatcaaaaat acttaagtag cgcgcaggga taagagggat ggtcccttaa
     7861 aggggagagc taattatccg gaaggattct gatgatgaac atcgaagaac tgcgtaaaat
     7921 tttttgtgaa gatggcctct atgctgtgtg cgttgaaaat ggaaatcttg ttagtcatta
     7981 ccgcattatg tgtttgcgaa agaatggggc tgcgttaatt aattttgtgg atgctcgggt
     8041 cacggacgga tttatcttgc gcgaaggtga gtttgtcact tcattacagg cattgaaaga
     8101 gatcggaata aaagctggct tttctgcttt ttcaggagaa taaactcatc tacaatcttg
     8161 cgcggggctg aactcccgct gagtaacacc gtgccaccgg agaaaaccga tggcacgcaa
     8221 cgcaaaatat tacaattctg ataattcgcc cgttcttgcc tgcacgcacg ggcggtattc
     8281 tcacgcattc aagtctgaat ggttccagca ccctccatgc actgcagaac aggccgaatg
     8341 gctgattcat tcttaccgca ggcgcgggtt cgaggttaag aaagctctca gtctcgacta
     8401 tcggcactgg ataatctctg tcaggctgcc ttattccgaa cgcccaccac gtgcgtcccg
     8461 cactttccag caacggatct ggaggtaacg tgcgggtatt acttagacct gttctggtgc
     8521 ctgagcttgg gctggtggtc cttaagccgg gccgtgaatc catacagata tttcataatc
     8581 ctcgagtgct ggtggaaccg gaaccaaaaa gcatgcgtaa tctgccatcc ggagtcgttc
     8641 ctgccgttcg ccagccgctg gcggaagaca aaacattgct gccgtttttt agtaacgaac
     8701 gggtgattcg tgctgctggc ggcgttggcg cattgtccga ctggctatta cgtcatgtta
     8761 catcctgcca gtggcctaat ggtgattacc atcacactga aacagtcatt caccgttatg
     8821 gtaccggcgc aatggtgttg tgctggcact gcgacaacca actgcgtgac cagacatcgg
     8881 aatcactgga gctgcttgct caacaaaatc tgacagcatg ggtgattgac gtcatccgtc
     8941 acgcaataag cggtacgcag gagcgggaat tatctttggc tgaattatcc tggtgggcgg
     9001 tctgcaatca ggtggtggat gcactacctg aggctgtatc gcgtcgttcg ctgggattac
     9061 cagcggaaaa aatctgctcg gtgtaccgcg aaagcgacat cgtaccggga gagcagaccg
     9121 ccaccagcat attgaaacaa cgcacaaaaa atcttgcacc gttgccttac gcccaccagc
     9181 aacaaaaatc accacaggaa aagacggtgg taagcatcac cgttgatcca gagtctccgg
     9241 aatctttcat gaagctgcct aaacgtcgcc gctgggttaa ggagaaatac acacgttggg
     9301 ttaagacaca gccgtgtgct tgctgcggta tgccagccga cgatccgcat catctgattg
     9361 gtcacgggca gggcggaatg ggaacaaaag cacatgatct ctttgtgttg cctttgtgca
     9421 gaaagcatca caacgagctg catacggata cagtggcatt tgaagataag tatggctccc
     9481 aactggagct gatatttcgt tttatcgatc gcgcgctggc aattggcgta ctggcgtaag
     9541 tggagaacga gcatgaacct tgaagcctta ccaaaatatt actccccaaa atctccaaaa
     9601 ttgagcgatg acgctccagc gacaggcacc ggttgtttaa caattacgga tgtaatggca
     9661 gcgcagggga tggtgcagtc gaaagcacca cttgggttgg ccttatttct ggcaaaagtt
     9721 ggtgttcagg accctcagtt tgcgattgaa ggcctgctaa attacgcgat ggcactggat
     9781 aacccgacat tgaacaaatt gagtgaagaa atccggttac agattattcc ttacctcgtg
     9841 aattttgcct ttgctgatta ctccaggtct gcggcaagta aggctcgctg tgagcattgt
     9901 tcaggtacgg gattttataa tgtattgcgc gaagtggtga aacactaccg acgcggggaa
     9961 tctgtaatca aggaagaatg ggtgaaggaa ctatgtcagc attgccatgg taagggtgaa
    10021 gtcagcacag cgtgcagagg gtgtaagggt aaagggattg ttctggatga aaaaagaacc
    10081 cggtttcatg gcgtaccggt atataagatt tgtgggcgtt gtaatggaaa ccggtttagt
    10141 cgtttaccga ccacgctggc acgacgtcat gtccagaagc tggtaccaga cctgaccgat
    10201 tatcagtggt ataaggggta tgcggacgtc attggtaaac tggtaacaaa gtgctggcag
    10261 gaagaagcat acgcggaagc gcaattgagg aaggtgacga gataaatgat ttttgctgaa
    10321 gatggcgaca tgatgtttgc atttttcaaa aaatatggat aaaatttttt caacgatggg
    10381 ctttgtatac ccgacgttaa gaaaaagtag aaaacccgct gatgagcggg ttttgtgctt
    10441 taaatggggc aatggtaatg ttgaa
//
LOCUS       DAZGJW010000155        10449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZGJW010000155 DAZGJW010000000
VERSION     DAZGJW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.155"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHKIK_004147"
     CDS             <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHKIK_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO9085208.1"
                     /translation="IIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGFGGV
                     LDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSLLSR
                     PLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAVAVI
                     ATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRAFDK
                     KDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVAVMLL
                     SLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIGTVD
                     AVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVPAVV
                     MIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIATITL
                     FY"
     gene            complement(1402..1899)
                     /gene="sspB"
                     /locus_tag="ACHKIK_004148"
     CDS             complement(1402..1899)
                     /gene="sspB"
                     /locus_tag="ACHKIK_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO9085209.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1905..2543)
                     /gene="sspA"
                     /locus_tag="ACHKIK_004149"
     CDS             complement(1905..2543)
                     /gene="sspA"
                     /locus_tag="ACHKIK_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO9085210.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(2938..3330)
                     /gene="rpsI"
                     /locus_tag="ACHKIK_004150"
     CDS             complement(2938..3330)
                     /gene="rpsI"
                     /locus_tag="ACHKIK_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO9085211.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3346..3774)
                     /gene="rplM"
                     /locus_tag="ACHKIK_004151"
     CDS             complement(3346..3774)
                     /gene="rplM"
                     /locus_tag="ACHKIK_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO9085212.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(3993..5120)
                     /gene="zapE"
                     /locus_tag="ACHKIK_004152"
     CDS             complement(3993..5120)
                     /gene="zapE"
                     /locus_tag="ACHKIK_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO9085213.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5314..5712
                     /gene="zapG"
                     /locus_tag="ACHKIK_004153"
     CDS             5314..5712
                     /gene="zapG"
                     /locus_tag="ACHKIK_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO9085214.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5866..7233
                     /gene="degQ"
                     /locus_tag="ACHKIK_004154"
     CDS             5866..7233
                     /gene="degQ"
                     /locus_tag="ACHKIK_004154"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO9085215.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7323..8390
                     /gene="degS"
                     /locus_tag="ACHKIK_004155"
     CDS             7323..8390
                     /gene="degS"
                     /locus_tag="ACHKIK_004155"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO9085216.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8453..9391)
                     /gene="mdh"
                     /locus_tag="ACHKIK_004156"
     CDS             complement(8453..9391)
                     /gene="mdh"
                     /locus_tag="ACHKIK_004156"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO9085217.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            9826..10296
                     /gene="argR"
                     /locus_tag="ACHKIK_004157"
     CDS             9826..10296
                     /gene="argR"
                     /locus_tag="ACHKIK_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO9085218.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2562 a         2659 c         2614 g         2614 t
ORIGIN      
        1 ataattatat ctgtcatcgt attaattacg atgggctatt tgatcctgaa aaactacaaa
       61 cctcaggtgg tgctggctgc cgcaggtatc ttcctgatga tgtgcggtgt ctggttaggg
      121 ttcggtggtg tactcgatcc cgccaaaagc agcggctact tgatcgtcga tatttataat
      181 gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctgtcgat tatggcagtg
      241 ggcggttatg cccgctacat ggagcgcaca ggagccagtc gcgcgatggt gagcttgcta
      301 agccgcccgt taaaactcat tcgctcgccg tatattattc tgtcggcaac ttacgtcatc
      361 ggccaaatca tggcgcagtt tattaccagc gcctccggcc tgggtatgtt gctgatggtc
      421 accttatttc cgacgctggt gagtctggga gtaagtcgcc tctctgcggt ggcggttatc
      481 gcaaccacga tgtccattga gtgggggatt ctggaaacga actccatttt tgcagcccag
      541 gtcgcgggaa tgaaaattgc cacttacttc ttccactacc agcttccggt cgcctcttgc
      601 gtcattatct cggtggcgat ctcccacttt ttcgtgcaac gcgcttttga caaaaaagat
      661 aaaaatatca atcacgaaca ggcagagcta aaagctctcg ataatgtccc gccgctctat
      721 tacgccattt tacctgtgat gccgttaatc ttgatgctcg gctcgctgtt cctcgcccac
      781 atcgggctga tgcagtcaga actgcatctg gtggcggtga tgttactgag tttgacggtg
      841 acgatgtttg ttgagttctt ccgcaagcat aacttgcgcg aaacaatgga cgatgtgcag
      901 gcgttttttg acggcatggg tacgcagttt gccaacgtgg taacgctggt ggtcgcgggt
      961 gaaatatttg cgaaaggctt aacgacgatt ggcactgttg atgcggttat caggggtgcg
     1021 gagcattctg gtctgggcgg tattggcgtg atgattatta tggcgctagt cattgccatt
     1081 tgtgccattg tgatgggctc tggcaatgcg ccgtttatgt catttgccag tcttattccg
     1141 aatatcgcag ccggactaca tgtaccagcg gttgtaatga ttatgccgat gcattttgcc
     1201 acgacgctag cgcgcgcggt ttcgccgatt actgcggtgg tggtcgttac gtcaggaatt
     1261 gcaggcgttt cgccttttgc ggtggtgaag cgaacagcga tccccatggc agtcggtttc
     1321 gtggtgaata tgattgccac aatcacgcta ttttattaag ccattaaaag acaaaacagg
     1381 ccaccagggc ctgtttttgt attacttcac aacgcgtaat gccggtcgac caccgcgtgg
     1441 tggctgcgga ggttcatcgt caggatgagt gtcatcatcg tgatctggct tgtcgccatc
     1501 aataaccgac ataacggttt cgttgtctgc cgatgcctct tcatcattca tgatgctggt
     1561 atcttcatcg taggcagctt caggctcaaa catcgtgcct gcgccatttt cacgggcgta
     1621 gatagccagc acggcagcca gcggcacaga aacctgacgc ggaatgccgc caaagcgcgc
     1681 gttaaagcgt acctcatcat tcgccagttc cagattgccg acagcacgcg gcgcaatgtt
     1741 gagtacgatt tgcccgtcac gcgcatattc cataggaacc tgcacgccag ggagcgtcac
     1801 atccaccacc aagtgcggcg tgagctggtt atccagcaac cactcataga atgcacgcag
     1861 cagataggga cgacgtggtg ttagctgtga caaatccata cagattaact ccggcccaga
     1921 cgcatttcac gttctgcttc agttaaagaa gcaaggaaag agtcacgctc aaagacgcgg
     1981 gtcatatagc ctttcagctc tttcgcaccc gggccgctga actcgatgcc cagttgcggc
     2041 agacgccaca gcagcggagc aagatagcaa tcgaccaggc tgaactcatc gctcaggaag
     2101 tacggcttct gaccgaagac cggcgcaatc gccagcagtt cttcgcgcag ttgcttacgt
     2161 gcggcatctg cttcagaagc tgaaccgttg atgatggtgt tcatcagcgt gtaccagtct
     2221 ttttcgatgc gatgcatgta cagacggctt tcaccgcgag ctaccgggta aacaggcatc
     2281 agtggcggat gcgggaaacg ctcatccaga tattccataa tgatgcgaga ttcccacagg
     2341 gtcagctcac gatccaccag ggtcggaacg ctctgattcg ggttgaggtc aatcagatcc
     2401 tgaggcggat tgtccttttc cacgtgttcg atctcgaaac ttacaccttt ctcagccagc
     2461 acaatgcgga cctgatggct atagatgtca gtaggaccgg aaaacagcgt cattaccgaa
     2521 cgtttgttgg cagcgacagc catgaaaacc tccaggtata gtcagaattt ttactgctac
     2581 cagccaccag gtggccagtc agaagttgtg ttacccagta aggaacgact ctctttgttc
     2641 gaaaatcaaa caaaaaatga gcaatacccg acatttgggc agaaaattgg atgatagttt
     2701 accagatttt gcgacctttg tggtgagtcg atgccggaaa tggggaaaaa gagatgcgct
     2761 ttagtctgaa atagttgact tagtccctta ttggcgatgt ggtttttgtt ttgcctgtct
     2821 gtcagacggt agcaaaaagc aactttccaa tttttacgct gattcagaat ttaaccataa
     2881 aaaaacccgc cgaagcgggt tttttcgcaa attgttttct gccggagcag aagccaatta
     2941 acgtttggag aactgcggac gacgacgtgc tttacgcaga ccgactttct tacgttcaac
     3001 ctgacgagcg tcacgagtaa cgaagccagc tttacgcagt tcagaacgca gggactcgtc
     3061 gtattccatc agagcgcggg tgataccgtg acggatcgca ccagcctgac cagagatacc
     3121 accaccttta acggtgatgt acaggtccag tttctcaacc atgtcgacca gttccagcgg
     3181 ctgacgaact accatgcgag cagtttcacg accgaagtac tgttccagag aacgttggtt
     3241 gattacgatt ttaccgttgc ccggtttgat gaaaacgcga gctgcggaac ttttgcggcg
     3301 accagtgccg tagtattgat tttcagccat tgcctataat cccgattaga tgtcaagaac
     3361 ttgcggttgc tgtgccgcgt ggttgtgctc gttacccgcg taaactttca gtttacggaa
     3421 catagcacga cccagcgggc cttttggcaa catgccttta accgcgattt caatcacacg
     3481 ctcaggacgg cgagcaatca tctcttcaaa ggtcgcttgt ttgataccac cgatgtggcc
     3541 ggtgtggtga tagtacactt tgtcagtacg cttgttgccg gttacagcaa ctttgtcagc
     3601 gttcagaacg atgatgtaat caccggtatc tacgtgcgga gtgtattccg ctttgtgctt
     3661 accgcgcagg cgacgagcca gttcagtagc cagacggccc agagttttac cggtcgcgtc
     3721 aacaacatac cagtcgcgtt ttacggtttc tggtttagct gtaaaagttt tcattaaaag
     3781 cttacccaat aaatagttac acgttggtga acacccaaac gtcttcaatt gttgaggttc
     3841 acacgacaaa gtccggcaaa cctacccctt cgaatagcct atgccagcac acaaaaagtt
     3901 ttgggaaaaa aactttcttg taacgtgggg tcgcaggatt atagagaagt cggggtcaaa
     3961 gatcgacccc tttttgtgat ttgtgacagg ttttaacccg ccaaatgctc gcgcttcaga
     4021 tactcttcgc tttgcatctc ttgcagacgt gacaggcaac gctggaactc aaacttcagc
     4081 cgatcgccct gataaatttc atacagcggc acttctgcac tcaccactaa tttgacatgg
     4141 cgctcgtaaa actcatccac cagcgcaata aagcgccgcg cttcgctctc catcaaccgc
     4201 gtcataactg gtacatcaaa caacatgacc gtatgaaaga gacgtgagag cgcaatatag
     4261 tcatgctgac tgcgggcgtc gacgcacagc gtagtaaaag agaccgccag cgtctggttc
     4321 tcgacgccca ttgtcgccaa tggccgatgg ttgatttcta acgtcggtga attttctcgt
     4381 ttcgcccccg ccagcgccaa ccatagttta tccatctgcg cccgggtttc atcgttgagt
     4441 ggcgaaagcc acagatgcgc ctgagtgagt gtacgcagtc gataatcaac accagcgtcc
     4501 acgttcatta catcacaatg ctgtttaatg gcatcgattg caggcagaaa acgcgcacgt
     4561 tgcaggccat ttcgataaag ttcatccggc ggaatatttg acgtcgctac cagggtaatg
     4621 ccgcgggcga acagggcttt catcagaccg ccaagtagca tggcgtcggt aatatcagaa
     4681 acaaaaaatt cgtcaaaaca gagcacgtca gtttcggctt taaagcgatc ggcaataatt
     4741 tccagcggat cggtctgccc ctgtaaggca gttagctctt cgtgcacccg cagcataaaa
     4801 cggtgaaagt gcaggcgctg tttccgctct cccggcaggc tttgatagaa aaggtccatc
     4861 agccaggttt tcccgcgtcc tacaccgccc cacatatata agccacgcac tggcgtatgc
     4921 tttgtgtctt cgcgtttacc ccacagctta ccgacccgcg ccattagccc actcgtcctg
     4981 ggggctggtg gcctgctatt gatgagttct tgataaataa tttccaggcg gctgacggcc
     5041 tctttttgaa cgtcgtcggg ttgatggctg ccttcattga gcgccttcag gtattgcgat
     5101 gttggggtaa cgctttgcat gatcttattg ttattccttg aataatcggt gcgccgttgt
     5161 tcacggttga cgaaaaaaag gccgttctac actacgcgat atgcagtcgg gattccactt
     5221 ctgtggaatt aacggttata gtggcataat cagccgcagg catggagcct gaagccaaca
     5281 ccctacggaa acaaaagaca acgggagatg ttcatgacct gggaatatgc gctaattggg
     5341 ttagtcgtcg gcatcattat tggtgctgtg gccatgcgtt ttggtaatcg taaactacgc
     5401 cagcaacagg cgttgcagta cgaactggaa aagaataaag ctgaactgga cgagtatcgc
     5461 gaagagctgg ttagccactt tgcccgcagc gcggaattac tggataccat ggcgcacgac
     5521 tatcgccagc tgtatcagca catggcgaaa agctccagca gcctgctgcc ggaactgtct
     5581 gctgaagcaa acccgttccg taatcgtctg gcagagtctg aagccagcaa cgatcaggca
     5641 ccggtgcaga tgcctcgcga ctattctgaa ggcgcatccg gcctgctgcg tactggcgcg
     5701 aagcgcgact aatttatttt tcgggcgcag ccattgcgcc ctcctcttct ctccctcccc
     5761 gactatcatt taatctggtg tctcattgtt agccgtctga aaattcaata acatcaaact
     5821 gttttgaatc tcttttctta tcattcaggt acgagagcag gaataatgaa aaaacaaacc
     5881 cagctgttga gtgcattagc gttaagtgtc gggttaactc tctcggcgtc atttcaggcc
     5941 gtcgcgtcga ttccaggcca ggttgccgat caggcccctc tccccagtct ggctccaatg
     6001 ctggaaaaag tgcttccggc agtggtgagc gtacgggtgg aaggaacggc cagtcaggga
     6061 cagaaaatcc cggaagaatt caaaaagttt tttggtgatg atttaccgga tcaacctgca
     6121 caacccttcg aaggtttagg ttccggtgtc atcatcaacg ccagtaaagg ctatgtgctg
     6181 accaacaacc atgtgattaa tcaggcacag aaaatcagta ttcagctcaa tgatgggcgc
     6241 gagtttgatg caaaactgat tggtagcgat gaccagagcg atatcgccct gttacaaatt
     6301 caaaacccga gcaaattaac gcaaatcgct attgccgact ccgataaatt gcgcgtcggt
     6361 gattttgccg tagcggtcgg taacccattt ggccttgggc aaaccgccac ctctggcatt
     6421 gtttccgcat taggccgcag cgggttgaat cttgaaggtc tggaaaactt tatccagaca
     6481 gatgcttcca ttaaccgcgg taactccggc ggtgcactat taaaccttaa cggtgagtta
     6541 attggcatca acactgcaat ccttgcgcct ggcggcggga gcgtcgggat tggatttgcc
     6601 atccccagta atatggcgcg aacactggcg cagcagctta tcgactttgg tgaaatcaaa
     6661 cgcggtttgt taggcatcaa aggcaccgag atgagtgccg atatcgccaa agccttcaac
     6721 cttgacgtgc agcgtggcgc gtttgtcagc gaagtgttgc caggttctgg ctcggcaaaa
     6781 gcgggcgtca aagcgggcga tattattacc agcctcaacg gcaaaccgct gaatagcttt
     6841 gctgagttgc gctctcgtat cgcgactaca gagccgggca cgaaagtgaa gcttggcctg
     6901 ctgcgtaacg gcaaaccact ggaagtagaa gtgacgcttg ataccagcac ctcttcgtcg
     6961 gccagcgctg aaatgatcac gccagcgctg gaaggtgcaa cgttgagcga tggtcagcta
     7021 aaagatggcg gcaaaggtat taagatcgat gaagttgtca aaggaagccc agctgctcag
     7081 gctggcttgc aaaaagacga tgtgatcatt ggcgtcaacc gcgatcgggt gaactcgatt
     7141 gctgaaatgc gtaaagtgct ggcggcaaaa ccggccatca tcgccctgca aattgtacgc
     7201 ggtaatgaaa gcatctatct gctgatgcgt taatgtcgta aaccgggcat caggcttacg
     7261 tgtgatgtcc ggttaactcg tggtatgctg ctgccgttcc cttttttaat gacgcctcca
     7321 tcatgtttgt gaagctctta cgttccgttg cgattggatt aattgtcggc gctattctgc
     7381 tggttgccat gccttcgctg cgcagcctta acccgctttc cactccgcaa tttgacagta
     7441 ccgatgagac gcctgccagt tataatctgg cggttcgccg cgccgcgcca gcggtggtta
     7501 acgtttacaa ccgtggtttg aacaccaact ctcacaacca gcttgagatc cgcaccctgg
     7561 gatccggtgt aatcatggat caacgcggtt atatcatcac caataaacac gtcatcaacg
     7621 acgccgatca gatcatcgtc gccttacagg atggacgtgt atttgaagca ttgctggtgg
     7681 gatctgactc tctaaccgat ctggcggtac ttaaaattaa tgccactggc ggtttaccta
     7741 ccattccgat caatgcacgt cgcgtaccgc acattggcga cgtggtactg gcgatcggta
     7801 acccgtacaa cctcgggcag accattaccc aggggattat tagtgctacg ggtcgaatcg
     7861 gtctgaaccc gaccgggcgg caaaacttcc tgcaaaccga tgcttccatt aaccacggta
     7921 actctggcgg cgcgctggtg aactcgctgg gagaactgat gggcattaac acgctgtcgt
     7981 ttgataagag taacgatggc gaaacgccgg aaggtatcgg ctttgcgatt cctttccagt
     8041 tagcaaccaa aattatggat aagctgatcc gcgatggtcg cgtgatccgc ggctacattg
     8101 gtatcggcgg acgcgagatc gcaccactgc acgcgcaggg cggtggtata gatcaactgc
     8161 aagggatcgt ggttaatgaa gtgtcacctg acggcccggc ggcgaatgcg ggtattcagg
     8221 tcaacgatct gattatttcg gtggataaca aaccggccat ctctgctctg gagacgatgg
     8281 atcaggtggc agaaattcgc cctggttcgg tgatcccggt tgtagtgatg cgtgatgata
     8341 agcagttaac gctgcaggtc accattcagg aatatccggc aaccaattaa gtcgagtgct
     8401 caaaacaaaa aaccggagtc tgtgctccgg ttttttatta ttcgctaatt aattacttat
     8461 taacgaactc ttcgcccagg gcgatatctt tcttcagcgt atccagcata ccttccagtg
     8521 cgctctgttc aaatgcgctc agggtaccga tagatttacg ctcttccacg ccgtttttac
     8581 ccagcagcag cggttgagag aagaaacggg cgtactgacc gtcgccttca acgtaggcac
     8641 attcgacaac gccttgttcg ccctgcagtg cacgaaccag agacagacca aaacgtgcag
     8701 ctgcctggcc catagacagg gttgcagacc caccaccggc tttcgcttca accacttcag
     8761 tacctgcgtt ctggatacgt ttggtcagat cagccacttc ctgctcggta aaactaacgc
     8821 caggaacctg tgacagcagc ggcagaatgg taacaccaga gtgaccgcca ataaccggca
     8881 cttcaacttc gcctggctgt ttgcctttca gttccgcaac aaaagtgttg gaacgaatga
     8941 tatccagcgt ggtaacgccg aacagtttgt ttttgtcata aacaccggct tttttcagca
     9001 cttcagcagc aatcgcaact gtggtgttaa ccgggttagt gataatacca atgcacgctt
     9061 tcgggcaggt tttcgcaact tgctgtacca ggtttttcac gatgccggcg ttaacgttaa
     9121 acaggtcgga acgatccata cccggtttac gcgctacacc tgcagagata agaacgacat
     9181 ctgcgccttc cagcgccgga gtcgcatctt caccagaaaa acctttgatt ttcacagcag
     9241 tagggatatg gctcagatcg acagccacac cgggagtcac tggagcgata tcatacagag
     9301 agagttctga acctgaaggc agttgggttt ttaacagtag tgcaagcgcc tggccaatac
     9361 cgccagcagc gccgaggact gcgactttca tcctaaactc cttattatat tgataaacta
     9421 agatatgttg ctccgctgcc gcgaccttaa tccacaaaat tgccgtttac aatgaccaca
     9481 tctcaagaat gtgtagtcac gcaagtttag cgtttatgca tttaattgcc gtaatcagga
     9541 acctaactta cgtaattaac agtcgcttat gcattagcgc aacattccaa caggtggtga
     9601 caatataccc taccgttcag ccaaaacaac atcaatttga taacaattaa tttactttta
     9661 aacagaattt gcatgccgtg acgcaggcat gtttctcaat aacgaaattt gataaaatcc
     9721 cgctctttca taacattatt tcagccttct tcagggctga ctgtttgcat aaaaattcat
     9781 ctgtatgcac aataatgttg tatcaaccac catatcgggt gacttatgcg aagctcggct
     9841 aagcaagaag aactagttaa agcatttaaa gcattactta aagaagagaa atttagctcc
     9901 cagggcgaaa tcgtcgccgc gttgcaggag caaggctttg acaatattaa tcagtctaaa
     9961 gtctcgcgga tgttgaccaa gtttggtgct gtacgtacac gcaatgccaa aatggaaatg
    10021 gtttactgcc tgccagctga actgggtgta ccaactacct ccagtccatt gaagaatctg
    10081 gtgctggata tcgactacaa cgatgcagtt gtcgtgattc ataccagccc tggcgcggcg
    10141 cagttaattg ctcgcctgct ggactcactg ggcaaagcag aaggtattct gggcaccatc
    10201 gctggcgatg acaccatctt taccacccct gctaacggtt tcaccgtcaa agacctgtac
    10261 gaagcaattt tagagctgtt cgaccaggag ctttaatctc tgccccgtcg cttctggcgt
    10321 cggggaaaat gttgcttatc cctctcattc cccttctttc tcctgcgata catttaacga
    10381 atagtgcgtt ttaccgcgac acatcattca caaaatgaat acatacggta aaaaaagcac
    10441 attatgcaa
//
LOCUS       DAZGJW010000156        10415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZGJW010000156 DAZGJW010000000
VERSION     DAZGJW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.156"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            36..611
                     /gene="ssuE"
                     /locus_tag="ACHKIK_004158"
     CDS             36..611
                     /gene="ssuE"
                     /locus_tag="ACHKIK_004158"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO9085219.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNCLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            604..1563
                     /gene="ssuA"
                     /locus_tag="ACHKIK_004159"
     CDS             604..1563
                     /gene="ssuA"
                     /locus_tag="ACHKIK_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO9085220.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGAVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAKVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1560..2705
                     /gene="ssuD"
                     /locus_tag="ACHKIK_004160"
     CDS             1560..2705
                     /gene="ssuD"
                     /locus_tag="ACHKIK_004160"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO9085221.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2716..3507
                     /gene="ssuC"
                     /locus_tag="ACHKIK_004161"
     CDS             2716..3507
                     /gene="ssuC"
                     /locus_tag="ACHKIK_004161"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO9085222.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3504..4271
                     /gene="ssuB"
                     /locus_tag="ACHKIK_004162"
     CDS             3504..4271
                     /gene="ssuB"
                     /locus_tag="ACHKIK_004162"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO9085223.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4478..7090)
                     /gene="pepN"
                     /locus_tag="ACHKIK_004163"
     CDS             complement(4478..7090)
                     /gene="pepN"
                     /locus_tag="ACHKIK_004163"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO9085224.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7356..8558
                     /gene="pncB"
                     /locus_tag="ACHKIK_004164"
     CDS             7356..8558
                     /gene="pncB"
                     /locus_tag="ACHKIK_004164"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO9085225.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8727..10127
                     /gene="asnS"
                     /locus_tag="ACHKIK_004165"
     CDS             8727..10127
                     /gene="asnS"
                     /locus_tag="ACHKIK_004165"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO9085226.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2352 a         2690 c         2801 g         2572 t
ORIGIN      
        1 aacaaaaaaa gaacaattgg ttataaggag agagtatgcg tgtcatcacc ctggcgggta
       61 gtcctcgctt tccttctcgc tccagctcct tgctggaata tgcgcgggaa aaactaaatt
      121 gcctggatgt agaggtttat cactggaatc tgcaaaactt cgccccggaa gatctgcttt
      181 acgctcgttt cgatagtccg gcactcaaga ccttcaccga acagctgcaa caggctgatg
      241 gactgatagt cgctacacct gtgtataaag ccgcctattc cggcgcgctg aaaaccctgc
      301 tcgacctgct gccagagcgc gctttgcaag gcaaagtggt gctaccgctg gcgacgggcg
      361 gtacggtggc tcatctgctg gcggtcgatt atgcccttaa gccagtttta agtgcgatga
      421 aagcccagga gatcctgcac ggcgtgtttg ccgatgactc acaagttatt gattaccatc
      481 acaaacccca gttcacgcca aatctgcaaa cccgtcttga taccgcgcta gaaactttct
      541 ggcaggcatt gcaccgccgc gatgttcagg ttcctgacct tctgtctctg cgaggtaatg
      601 tccatgcgta acatcattaa actggcgctg gcgggattgc ttagcgtctc tacttttgcg
      661 gttgctgcag aatcctcgcc tgaagcgtta cgtataggct atcagaaagg cagtattggt
      721 atggtattgg caaaaagcca ccagttactg gaaaaacgct atccgcaatc aaaaatctcc
      781 tgggtggagt tccccgcggg tccgcaaatg ttggaagcgt taaacgttgg cagtattgat
      841 ctcggcagta ccggggatat tccgccaatc tttgcccagg ctgccggggc tgatttggta
      901 tatgtgggcg tcgagccacc gaagcccaaa gccgaagtga ttctggtggc agaaaacagc
      961 ccgatcaaaa ccgtagccga tcttaaaggt cacaaagttg cctttcagaa aggttccagt
     1021 tcacacaacc ttttactgcg tgcactgcgt caggccggac ttaagtttac cgatatccaa
     1081 cccacttacc tgacgcccgc tgatgcccgc gccgcgttcc agcaaggtaa cgttgacgcc
     1141 tgggctatct gggatcccta ctactctgct gcattattac agggcgccgt gcgggtgctg
     1201 aaagacggca ccgatctcaa tcaaactgga tcgttttatc tggcagctcg tccctatgca
     1261 gaaaaaaacg gcgcttttat tcagggcgta ctcgcaacct ttagtgaggc cgatgcgtta
     1321 acccgcagcc agcgcgagca aagcatcgct ttactggcaa aaacgatggg cttaccggca
     1381 ccggtgattg cctcttactt agatcatcgc cctcctacca ccatcaaacc ggttaacgcc
     1441 aaggttgccg ccttacagca gcaaacggca gatctgtttt atgaaaatcg tctggtgccg
     1501 aaaaaagtcg atattcgcca gcgcatctgg cagcccactc aactggaagg aaaacaatta
     1561 tgagtctgaa tatgttctgg tttttaccga cccatggtga cgggcattat cttggaacgg
     1621 aagaaggttc acgcccggtt gatcacggct atctgcaaca aattgcgcaa gcggcggatc
     1681 gtctgggcta taccggtgtg ctgatcccaa cggggcgatc gtgtgaagat gcgtggctgg
     1741 tggcggcgtc gatgatcccg gtgacgcagc ggctgaagtt tcttgtcgcc ctgcgcccca
     1801 gcgtaacctc acctaccgtt gccgctcgcc aggccgccac gcttgaccgt ctctcaaatg
     1861 gtcgtgcgtt gtttaatctg gtcacaggca gcgatccaca agagctggca ggcgacggcg
     1921 tgttccttga tcatagcgag cgctacgaag cctcggcgga atttacccag gtctggcggc
     1981 gcttattgct gggggaaacc gtcaatttca acggtaaaca tattcatgtg cgcggagcaa
     2041 aactgctctt cccgccgatt caacagcctt atcctccgct ttactttggc ggatcgtcag
     2101 acgttgccca ggagctggcg gcagaacaag ttgatctcta cctcacctgg ggcgaaccgc
     2161 cagaactagt aaaagagaag atcgaacaag tgcgggcgaa agctgccgcg catggacgca
     2221 aaattcgttt cggtattcgt ctgcatgtga ttgttcgtga aactaacgac gaagcgtggc
     2281 aggccgccga gcggttaatc tcgcatcttg atgatgaaac tatcgccaaa gcacaggccg
     2341 cattcgcccg gacggattcc gtaggacaac agcgaatggc ggcgttacat aacggcaagc
     2401 gcgacaatct ggagatcagc cccaatttat gggcgggcgt tggcttagtg cgcggcggtg
     2461 ccggaacggc gctggtgggc gatggtccta cggtcgctgc gcgaatcaac gaatacgccg
     2521 cgcttggcat cgacagcttt gtgctttcgg gctatccgca tctggaagaa gcgtatcggg
     2581 tcggcgagtt gctgttccca catctggatg tcgccattcc ggaaattccc cagccgcagc
     2641 cgctgaatcc gcaaggcgaa gcggtggcga atgattttat cccccgtaaa gtcgcgcaaa
     2701 gctaaggaag aaataatggc aacgccagtg aagaagtggt tattgcgcgt tgccccctgg
     2761 tttttaccgg tgggcatcgt ggcggtgtgg caactggcct cctcagttgg ctggctgtcg
     2821 acgcgtattt tgccctcacc ggaaggggtg gtgacggcgt tctggacgct ctccgccagc
     2881 ggcgaactgt ggcagcatct ggcgatcagt tcctggcggg cgctgattgg cttttcaatt
     2941 ggcggatcgc tggggttgat tttgggactg attagcgggc tatctcgctg gggagaacgg
     3001 ctgctggata cctcaattca gatgttgcgc aacgtaccgc atctggcgct gatcccgctg
     3061 gtgattttgt ggtttggcat tgatgaatcc gcaaaaatct ttctggtggc gctcggcacg
     3121 ctattcccca tttatatcaa cacctggcat ggcatccgta atatcgatcg cggtctggtg
     3181 gaaatggcgc gtagctatgg attatccggc ataccgctgt ttatccatgt gatcctgcct
     3241 ggtgccctgc cctcaattat ggtcggcgtg cgttttgcgt tgggcctgat gtggctgacg
     3301 ctgattgttg ccgaaaccat ttctgccaat tcaggcattg gttatctggc gatgaatgcg
     3361 cgggagtttt tgcaaacgga cgtggtggta gtcgctatta ttctttacgc cctgctcggc
     3421 aaacttgctg acgtcagcgc gcagctgtta gagcgcctct ggttgcgctg gaacccggct
     3481 tatcatttga aggaggccac ggtatgaata ctgctcgtct gaaccagggc acaccattgt
     3541 tgctcaatgc agtaagcaaa cattacgcgg aaaatatcgt cctgaaccaa ctggatttac
     3601 atattccggc aggtcagttt gtggcggtgg tgggccgcag cggtggtggc aaaagtaccc
     3661 tgctgcgcct gctggcaggt ctggaaacgc caaccgcagg cgatgtgtta gcgggcacca
     3721 caccgctggc tgagattcaa gaagacacgc gaatgatgtt tcaggatgcg cgtctgctgc
     3781 cgtggaaatc ggtgattgat aacgttgggt taggccttaa aggccagtgg cgcgatgccg
     3841 cgcgtcgggc gttggctgca gtaggactgg agaatcgcgc cggggaatgg cctgccgcac
     3901 tttctggcgg gcagaaacag cgtgtggcgc tggcaagagc gttgattcat cgaccgggtt
     3961 tattgttgct tgatgaaccg ctcggggcgc tggacgcctt aacgcgactc gagatgcagg
     4021 atttgattgt gtcactttgg cagcagcacg gctttaccgt actgttggtg acgcatgatg
     4081 tgagcgaagc ggtagcaatg gctgaccggg tgttgttaat tgaagaggga aaaattggtc
     4141 tggatttgac agtggatatc ccccgtccgc gtcgattagg gtcggtgcga ttagcggagc
     4201 tggaagcgga agtattgcaa cgggtaatgc ggcgtgggca ttctgaacaa ctgattcggc
     4261 gtcatggtta atatgccgga tgtggcgcac aaaaccagca ttttcaacgg actagtaggc
     4321 ctgataagac acgtcaagca tcgcatcagg cagttgggca cggctgccgg atgcggcgtg
     4381 aacgccttat ccagcctaca aaaccagcaa tttcaacgga gttgtaggcc tgataagacg
     4441 cgtcaagcgt cgcatcaggc attttggctc aaaagtatca agccagtgct ttggtgatct
     4501 tctcgtacag atcgccagag agattttcca gccctttcaa ctgttccagc gccgcgcgca
     4561 ttttctcctg acgtttggca tcataacgtt tcaggcgaat cagcggttca atcagacgtg
     4621 aagctacctg cgggttacgg ctgttgaggt cggtaagcat ttccaccagg aactggtaac
     4681 cgctgccatc ttcggcatgg aacgctgccg ggttgctgcc cgcaaacgca ccaatcaacg
     4741 aacgaatacg gttcgggttg ctcatggtaa atgagcgatg ctgcaacaga ccacgcaccg
     4801 tctccagcac attcgccgcc gggctggtgg cttgcaggat aaaccattta tccatcacca
     4861 gaccgtcctg atgccactta tcgtcgtact cctgcatcag cgcgtcacgg caaggcagct
     4921 gtgcagcaac cgccgcagaa agcgctgcca gcgcatcggt catattgttt gcttcatggt
     4981 attgcttgct caccagcacg tcagccagat gcgtttcacc aaaagcaagg aagcgcaggc
     5041 aggcattacg cagagtgcgt tttgcaatat cttcatgctc aacacggtac tcgctctggt
     5101 aattcgcgtt gtaaatagcc agcagctcat ccgccagttc agtcgccaga gtacgagtga
     5161 gtgcttcgcg tacttcggca atagcaatcg ggtcgatgat atcgaacagt tcagccattt
     5221 cattgacaga aggcagcgtc aggatttctg ccgccagcgc cggatcaatc ttctcatcga
     5281 gcagcaccgc gcggaaagcg tcagccacat gtaccggcag agacagcggc tgcccttgct
     5341 gatgacgggc gacgttcagc ttgatgtagg ttgccagcaa actttgcgcc gcatcccaac
     5401 gggagaaatc attacgcgca tgacgcatca ggaaggtcag ttgctgatcg ctccacttat
     5461 attccagttt cactggcgca gagaattcgc acagcagcgc aggcaccggc tggaagtaga
     5521 cattatcaaa aacaaaggtc tgttccgcct gggtgacgtt cagcacggaa ttcaccggat
     5581 gaccgccttt ctgcaacggg atcactttgc cttcgttgtc atacaattcg atggcaaatg
     5641 gaatatgcag cggctgtttt tctgcctgat ccggcgtggc tggcgtgcgc tggctgatgg
     5701 tcagggtgta ctgctcggtt tccggattgt agtcgtcttt gacggtcaca atcggcgtgc
     5761 cggactggct gtaccaacgg cggaaatggg agagatcgac attcgacgca tcttccatcg
     5821 cctgcacaaa gtcgtcgcag gttgccgcgc tgccatcatg gcgctcgaaa tagagttgca
     5881 tccctttctg gaagttttct tcgcccagca gggtgtggat catgcgaatc acttccgcgc
     5941 ccttctcgta aacggtcagg gtgtagaagt tgttcatctc aatgaccata tccgggcgga
     6001 tcgggtgcgc catcgggctg gcgtcttccg caaactgcaa tccgcgcatg gtgcgcacat
     6061 tattgatacg gtttaccgcg cgggaaccaa ggtcagagct gaattcctga tcgcggaaga
     6121 cggttaaacc ttctttcagg ctgagctgga accagtcgcg gcaggtcact cggttaccgg
     6181 tccagttatg gaaatattca tggccgataa cgcgttcaat atcgagataa tctttgtcgg
     6241 tggcggtgtc ggtgcgggcc aacacatatt tggagttaaa gatattcaga cccttattct
     6301 ccattgcgcc catattgaag aaatccaccg cgacgatcat atagatgtcg aggtcatact
     6361 ccaggccaaa gcgttcttca tcccatttca tggagttttt cagcgaggtc atcgcccacg
     6421 gcgcgcgatc aaggttgccg cgatcgacgt acagctccag tgctacttcg cgaccagaac
     6481 gcgtggtaaa ggtgtcgcgc agtacatcaa agtcgcctgc caccagcgca aacaggtagc
     6541 acggtttcgg gaacgggtcc tgccactgta cccaatggcg tccgttttcc agttcacctt
     6601 gcgcaacgcg gttaccgttg gaaagcagga agggatattt ggttttatcg gcaataattt
     6661 tggtggtaaa acgcgccagc acgtccgggc ggtcgagata atacgtaata tggcggaaac
     6721 cttcggcttc acactgggtg caaagcgcat cgcctgactg ataaagccct tccagcgccg
     6781 tattcgccgc cgggcttatt tcattaatga tcttaagcgt aaaacgctcc ggcaaattac
     6841 tgatgaccag tgcgccctct tcttctttcc aggcggtcca cggctcatca ttaatatgaa
     6901 cagaaaccag tttgaggtct tcgccgttga gacgaagcgg agcatctgat gcaccatgac
     6961 ggacagcctg gctgaccgcg gtaacgaccg tcttttgcgc gtcgaggtca aaggtcaagt
     7021 caatatcagt aatctggtaa tccggcgcac gataatcgtg acggtatttg gcttgtggct
     7081 gttgagtcat aaataacctt tagcatcttt tatagagtct ggtgttcagt ctattcctgt
     7141 tgcgtaaatc gcgctatgca gaatcttcat cttttcaggt acaaacgcct ttattgctac
     7201 atttttataa catacaccgc gtaatgccat cgaccagaaa ggtggcatat ggtgtgatcg
     7261 gggttcaata aattgcgaaa caaggtatac tccagcagtt cctgaagatg tttattgtac
     7321 taaacgctcc tgtacgagga cgctactgcg cacctatgac acaattcgct tctcctgttc
     7381 tgcactcgtt gctggataca gatgcttata agttgcatat gcagcaagcc gtgtttcatc
     7441 actattacga tgtgcatgtc gcggcggagt ttcgttgccg aggtgacgat ctgctgggta
     7501 tttatgccga tgctattcgt gaacagattc aggcgatgca gcacctgcgc ctgcaggatg
     7561 atgaatatca gtggctctct gccctgcctt tctttaaggc cgactatctt aactggttac
     7621 gcgagttccg ctttaacccg gaacaagtca ccgtatccaa cgataatggc aagctggata
     7681 ttcgtttaag cggcccgtgg cgtgaagtca tcctctggga agttcctttg ctggcggtta
     7741 tcagtgaaat ggtacatcgc tatcgctcac cgcaggccga cgttgcgcaa gccctcgaca
     7801 cgctggaaag caaattagtc gacttctcgg cgttaaccgc cggccttgat atgtcgcgct
     7861 tccatcttat ggattttggc acccgtcgcc gtttttctcg cgaagtacaa gaaaccatcg
     7921 ttaagcgtct gcaacaggaa tcctggttcg tgggcaccag caactacgat ctggcgcgtc
     7981 ggctttccct cacgccgatg ggaacacagg cacacgaatg gttccaggca catcagcaaa
     8041 tcagcccgga tctagccaac agccagcgag ctgcacttgc tgcctggctg gaagaatatc
     8101 ccgaccaact tggcattgca ttaaccgact gcatcactat ggatgctttc ctgcgtgatt
     8161 tcggtgtcga gttcgccagt cgctatcagg gcctgcgtca tgactctggc gacccggttg
     8221 aatggggtga aaaagccatt gcacattatg aaaagctggg aattgatcca cagagtaaaa
     8281 ctctggtttt ctctgacaat ctggatttac gcaaagcggt tgagctatac cgccacttct
     8341 cttcccgcgt gcaattaagt tttggtattg ggactcgcct gacctgcgat atcccccagg
     8401 taaaacccct gaatattgtg attaagttgg ttgagtgtaa cggtaaaccg gtggcgaaac
     8461 tttctgacag ccctggcaaa actatctgcc acgataaagc gtttgttcgg gcgctgcgca
     8521 aagcgttcga ccttccgcat attaaaaaag ccagttaata tcatcaggga gctaatcggc
     8581 tccctttttt tacctttaat tccgaaatct ttcgctgcat ttgcgaattc tgcttgtctg
     8641 attgcagatg ccaggtaaca taggtatccc cccatttacg ggtggacaag tgtttatttt
     8701 ttccgactat taacagagag aatattatga gcgttgtgcc tgtagccgac gtactccagg
     8761 gccgcgtagc cgttgacagc gaagtcaccg tacgcggatg ggtacgtacc cgccgagatt
     8821 caaaagctgg catctccttc ctcgccgttt atgacggttc ctgctttgat cctgtacagg
     8881 ctgtcatcaa taattctctg cccaattaca atgaagacgt cctgcgtctg accaccggct
     8941 gctcggtcat tgtgacgggt aaagtcgtgg cgtcgccggg ccaggggcaa caatttgaaa
     9001 ttcaggccag caaggttgaa gttgctggtt gggttgaaga tccagacact tacccgatgg
     9061 cggcaaaacg ccacagcatt gagtatctgc gtgaagtcgc tcacctgcgt ccgcgcacaa
     9121 acctgattgg tgccgtcgcg cgcgttcgtc atacgctggc gcaggcgctg catcgcttct
     9181 ttaacgagca gggattcttc tgggtttcaa cgccactgat taccgcatct gataccgaag
     9241 gtgcaggcga aatgttccgc gtttctacgc tggatctgga aaacctgccg cgtaacgatc
     9301 agggcaaagt ggatttcgac aaagacttct ttggtaaaga gtctttcctg accgtatctg
     9361 gccagttgaa cggcgaaacc tacgcttgcg cattatccaa aatttatacc ttcggcccga
     9421 ctttccgtgc tgaaaactcc aacaccagcc gtcacctggc ggaattctgg atgctggagc
     9481 cggaagtggc gtttgctaac ctgaacgata tcgcgggtct ggctgaagcc atgctgaaat
     9541 atgtcttcaa agcggttctc gaagaacgcg cagacgacat gaaattcttc gctgaacgcg
     9601 tagataaaga tgccgtttca cgtctggaac gcttcattga ggccgatttc gcgcaggtgg
     9661 attacaccga cgcagtgacc attctcgaaa actgcggcag gaagtttgaa aacccggttt
     9721 actggggcgt cgatctctct tctgagcatg agcgttatct ggcggaagaa cactttaaag
     9781 caccggtagt ggttaaaaac tatccgaaag atattaaagc gttctatatg cgcctcaacg
     9841 aagacggtaa aaccgttgcg gctatggacg ttctggctcc gggcatcggt gagatcattg
     9901 gtggctccca gcgtgaagaa cgtctggacg ttctggacga gcgtatgctg gaaatgggcc
     9961 tgaataaaga agattactgg tggtatcgcg atctgcgtcg ctacggtact gttccgcatt
    10021 caggtttcgg tcttggtttt gaacgcctga ttgcttacgt aactggcgtg caaaacgtgc
    10081 gtgatgtgat tccgttccca cgtactccgc gcaacgccag cttctgattt tcttcctcaa
    10141 agagccagcg cctgctggct cttttttatc ctttctgtca agttatctgt ttgttaagtc
    10201 aagcaatcta tttgcaaccc cgccataacg atcatcctgt tacggaatat tacattgcaa
    10261 catttacgta caaaaaataa cccgcattct tattgcggat tagttttttc ttagctgata
    10321 gcacaatttt catactattt tttggcattc tggatgtctg aaagaggatt ttgtgccagt
    10381 tcgataaagt ttccatcaga aacaaaattt ccgtt
//
LOCUS       DAZGJW010000157        10321 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZGJW010000157 DAZGJW010000000
VERSION     DAZGJW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.157"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(212..2785)
                     /gene="clpB"
                     /locus_tag="ACHKIK_004166"
     CDS             complement(212..2785)
                     /gene="clpB"
                     /locus_tag="ACHKIK_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO9085227.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2915..3646)
                     /gene="yfiH"
                     /locus_tag="ACHKIK_004167"
     CDS             complement(2915..3646)
                     /gene="yfiH"
                     /locus_tag="ACHKIK_004167"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO9085228.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="ACHKIK_004168"
     CDS             complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="ACHKIK_004168"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO9085229.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGELVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4758..5495
                     /gene="bamD"
                     /locus_tag="ACHKIK_004169"
     CDS             4758..5495
                     /gene="bamD"
                     /locus_tag="ACHKIK_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO9085230.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5766..6107
                     /gene="raiA"
                     /locus_tag="ACHKIK_004170"
     CDS             5766..6107
                     /gene="raiA"
                     /locus_tag="ACHKIK_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO9085231.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6210..6335
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6211..6258
                     /gene="pheL"
                     /locus_tag="ACHKIK_004171"
     CDS             6211..6258
                     /gene="pheL"
                     /locus_tag="ACHKIK_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO9085232.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6357..7517
                     /gene="pheA"
                     /locus_tag="ACHKIK_004172"
     CDS             6357..7517
                     /gene="pheA"
                     /locus_tag="ACHKIK_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO9085233.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="ACHKIK_004173"
     CDS             complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="ACHKIK_004173"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO9085234.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="ACHKIK_004174"
     CDS             complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="ACHKIK_004174"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO9085235.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9972..>10321
                     /gene="yfiL"
                     /locus_tag="ACHKIK_004175"
     CDS             9972..>10321
                     /gene="yfiL"
                     /locus_tag="ACHKIK_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO9085236.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENAST"
BASE COUNT         2641 a         2732 c         2427 g         2521 t
ORIGIN      
        1 cggaattctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagcga aaaatataaa aactttatcg
      121 ttcgctcatt tttcaggcaa agcatcttaa atatagtctt ttccgtctaa cttatagaca
      181 aaaacgagcc ccgaagggct cgttttatca tttactggac ggcgacaatc cggtcttcat
      241 taacttccag gcgaatcact ttacccggaa ccaattcacc agacagtatt tgctgtgcca
      301 gcgggttttc gatctgctgc tggatagcac gtttcaaagg acgtgcacca tagactggat
      361 cgtaaccgtt ctcgctcagc agtttcagcg cctcgtcaga aatgtggatt tcataaccac
      421 gttcttccag acgtttgtac agacgtttca actgaatctg cgcaatcgag gcaatgtgct
      481 gttcacccag cggatggaag accaccactt catcaatacg gttaatgaat tccggacgga
      541 agttatggct taccacaccg agcaccagct ctttcatgtg cgcataatcc agttcaccga
      601 agcgttcctg aatcaaatcg gaaccgaggt tagaggtcat aatgacaacc gtattacgga
      661 agtcgaccgt tctcccctgc ccgtcagtca gacgaccatc atccagtacc tgcaacagaa
      721 tgttgaagac atccggatgc gctttttcca cttcatccag caggatgacg gaatacggac
      781 gacgacgcac agcttcggtc aggtagccac cttcttcata accgacatat cccggaggcg
      841 caccaaccag acgagacacc gagtgtttct ccataaactc ggacatatcg atacggacca
      901 tcgcctcgtc gctatcaaac ataaagttcg ccagcgcctt acaaagctca gttttcccca
      961 cacccgttgg gccgaggaac aggaatgaac caatcgggcg atttggatcc gccaaccccg
     1021 cacggctacg gcgaatagcg ttagataccg catcaaccgc ttcgttctga ccaatgacgc
     1081 gatggtgcag ttcttgctcc atacgcagca gtttttcgcg ctcgctttcc atcatgcgag
     1141 aaaccggaat ccccgtccaa cgcgccagca cttcagcaat ttcggcgtcg gtcactttat
     1201 tacgcaacag acgcatagtt ttgccttcga gctgcgttgc ggcttccagt tgcttttcca
     1261 gttccgggat tttgccgtat tgcagttcag acatccgcgc caggtccccc acacggcgag
     1321 cctgttcaat agcgattttc gcctgttcca gttccgcttt aatggtctgc gtaccagaaa
     1381 gcgatgcctt ctctgctttc cactcttctt ctaactcgga gtactgacgt tctttgtcgc
     1441 tcagttcttc gttgagcata tccaggcgtt ttttactggc ttcatcagac tctttcatta
     1501 acgcctgttg ttccagtttg agctggatga tacgacgatc gagtcggtcg agttcttctg
     1561 gttttgagtc aatctgcata cgaatgctgg atgctgcttc atcgatcagg tcgatggctt
     1621 tatccggcag ctgacggtca gcaatgtagc gatgagacaa cgtcgccgct gcaacaattg
     1681 ccgggtcagt aatttgcaca tggtggtgca attcgtaacg ttctttcagg ccacgcagaa
     1741 tcgcaatggt atcttcaaca gaaggctcgg caacaaacac tttctggaaa cgacgttcca
     1801 gcgcagcatc tttttcgatg tactggcgat attcgtcaag cgtcgtggca cctacgcagt
     1861 gcaattcacc acgcgccagc gccggtttca gcatgtttcc ggcgtccatt gcgccatcgg
     1921 ctttacccgc gccgaccatg gtatgtaatt cgtcgataaa caggatgacg ttgccttcct
     1981 gtttggcaag atcgttaagc acgcctttta aacgttcttc aaactcaccg cgatatttcg
     2041 ccccagccac cagcgcgccc atatccagcg ccagtacgcg gcggcctttc aatccttccg
     2101 gcacttcgcc gttgataata cgctgcgcca gaccttcaac gatggcagtt ttaccgacgc
     2161 cgggttcacc aatcagtacc gggttatttt tagtacgacg ttgcagcacc tgaatggtac
     2221 ggcgaatttc ttcatcacga ccaatcaccg gatcgagttt gccctgttcg gctcgttcgg
     2281 taaggtcgat ggtatatttt ttcaaagcct gacgttggtc ttcagcacct tgatcgttca
     2341 cgctttcacc tccacgcatt tgttcaatcg cttgagtaat gttggcggtg gtcgcccctg
     2401 ctgctttcag gatgtcggcc agcgtgccgc gagactcaag tgccgccaga acgaacagtt
     2461 ctgacgagat aaagttatca ccacgttttt gcgccagctt gtcgcaaaga ttaagaacgc
     2521 gcaccagatc ctgtgatggc tggacatcac caccagtacc ttcaacctgc ggtaaacgat
     2581 ttaatgcctg attgatatct gtgcgcaact ggccagcatt tatgccagcg gatgttaata
     2641 aaggactaac cgaaccccct tcctgattca gcagggcgct cattaaatga agtggttcga
     2701 taaattggtt gtcgtgcccg agtgcaagtg attgggcatc ggcaagagca agctggaatt
     2761 tattagtaag acgatccaga cgcataactc ctcccataac ggatcaaagt tgctactgga
     2821 gattaaatga ggtcatccct caattattca aggttattga ccagattaat gtgaaaagaa
     2881 aatcacgcgt accggatcgt cttgattctt taggttatat cagccaaatg aaacttgcca
     2941 tacgaccggt ggtcttgtcg cgacgataag agaagaaagt ctcattttcc gtatatgtac
     3001 aacggtcgcc gccgaaaatt tgctcaacac ccacgttcgc cagacgctgc cgggcaagct
     3061 gataaatatc cgccagatac ttatcaccat gctgaatgaa agctgcactt gctttagcgt
     3121 ctactgccat aaacgcctcg cgaacctcca cccccacttc gaacgcgcgt ggaccaattg
     3181 ccggccctaa ccaggcgaga atattttccg gattatcagc aaaacaggaa accgtctctt
     3241 ccagcacgcc tgcgcacagt ccacgccagc cagcatgagc ggcggcgact tccgttcccg
     3301 ctcgattgca aaacagcaca gggaggcagt cggcagtcat cactgcgcaa accgtgccgg
     3361 gcgtattgct ataagaggca tctgctcgtt ttgaggcata aggttcgcca gtaagcttaa
     3421 gcacatcttt gccgtgtacc tgctcaagcc agaccggttt agaaggcaaa ttgcccgcag
     3481 caaaaagtcg cttgcgattc tcctcaacgt gatccgggtt atcgccacaa tgggcaccga
     3541 ggttgagcga gtcatacggg ggcaagctca cgccgccgat acgagaagag ctacaggccg
     3601 caacaccttt tggtagcggc cactgcggga caatcagctt actcataacc agtccacttc
     3661 atccttatgc tcttcgaaat cggcgcgcat cacctcaatc agctccacca tatcttgtgg
     3721 aataggcgca tgccattcca tttcgatgcc ggagatcggg tgataaagac gcagcatggt
     3781 tgcatgaagc gcctggcggt caaacttacg cagcatggag ataaatgctt ccgaagcacc
     3841 ttttggcgga cgcggacggc caccataaac cggatcgccc accagcggat gagtgatatg
     3901 ggccatatgc acgcggatct ggtgcgtacg tccagtttcc agacgcaacc gcagacgcgt
     3961 gtgcacacgg aagtgttcca tgatgcgata gtgagtcacc gccggtttgc ccatcggatg
     4021 caccgccatg tgagtacgtt tggtcgggtg gcggctgatg ggttcatcta ctgtaccgcc
     4081 agcggtcata tgaccaatcg ccaccgcttc atactcacga gtaatttcac gccgttgcaa
     4141 agattcgact aaacgcgtct gagccggaac ggtttttgcc acaaccatca ggccagtggt
     4201 gtctttatcc agacgatgga cgatgcccgc acgtggtaca tcggcaatgg gcggatagta
     4261 atgaagcaac gcattcagta ccgtgccatc cgggttaccc gcgccaggat gtaccaccag
     4321 gtcgcgcggt ttattaatga cgatgatatc ttcatcttca tagacgatat ccagcgggat
     4381 atcctgcggt tcaaaacgcg cttcttcttc aatctcagca ttgatggcaa ccagctcgcc
     4441 acccaatact ttttctttcg gcttatcaca aactttgccg tttaccagca cgcgctggtc
     4501 gaggatccat tcttttattc gcgaacgtga ataatccggg aacatttcgg ccaaagcctg
     4561 atctaagcgt tgaccgagtt ggttttcgga caccgttgca gtgagctgta ctcgttgtgc
     4621 catatatact gcttcttcgt ttatcgttgg ggttttacgg ctttgccgtt taatatagtg
     4681 tgctattgta gctggtctta accgggagca ggaacagaga atctcccgta ttacattttg
     4741 aggaaagtca aaacgtcatg acgcgcatga aatatctggt ggcagccgcc acactaagcc
     4801 tgtttttggc gggttgctcg gggtcaaagg aagaagtacc tgataatccg ccaaatgaaa
     4861 tttacgcgac tgcacaacaa aagctgcagg acggtaactg gagacaggca ataacgcaac
     4921 tggaagcgtt agataatcgc tatccgtttg gtccgtattc gcagcaggtg cagctggatc
     4981 tcatctacgc ctactataaa aacgccgatt tgccgttagc gcaggctgcc atcgatcgtt
     5041 ttattcgcct taacccgacc catccgaata tcgattatgt catgtacatg cgtggcctga
     5101 ccaatatggc gctggatgac agtgcgctgc aagggttctt tggcgttgac cgtagcgatc
     5161 gcgatcctca acatgcacga gctgcgttta gtgacttttc caaactggtg cgcggctatc
     5221 caaacagtca gtacaccacc gatgccacca aacgtctggt attcctgaaa gatcgtctgg
     5281 cgaaatatga atactccgtg gccgagtact atacagaacg tggcgcatgg gttgccgtcg
     5341 ttaaccgcgt agaaggaatg ttgcgcgact acccggatac ccaggctacg cgtgatgcgc
     5401 tgccgctgat ggaaaatgca taccgtcaga tgcagatgaa tgcgcaagct gaaaaagtag
     5461 cgaaaatcat cgccgcaaac agcagcaata cataacagaa acctgaaata caaaacggca
     5521 gcccttgagc tgccgttttt ttattctgtt agttgtgaaa ctgaagcgat ttagtcacta
     5581 tcgatctcat caaatatggc tcgctttgag atattcctca agtaaaaaaa cacctcttcc
     5641 tgcgatttct cacaaaaaag attcgttgac aaaaagtgac aaaattatga gattcccatc
     5701 acacattttg acatcaggag cggtatgctg aattcaccaa gacgggaaga caagaggtaa
     5761 aatttatgac aatgaacatt accagcaaac aaatggaaat tactccggcc atccgccaac
     5821 atgtcgcaga ccgtctcgcc aaactggaaa aatggcaaac acatctgatt aatccacata
     5881 tcattctgtc caaagagcca caagggtttg ttgctgacgc cacaatcaat acacctaacg
     5941 gcgttctggt tgccagtggt aaacatgaag atatgtacac tgcaattaac gaattgatca
     6001 acaagctgga acggcagctc aataaactgc agcacaaagg cgaagcacgt cgtgccgcaa
     6061 catcggtgaa agacgccaac ttcgtcgaag aagttgaaga agagtagtcc tttatattga
     6121 gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac agcgtgaaaa cagtacgggt
     6181 actgtactaa agtcacttaa ggaaacaaac atgaaacaca caccgttttt cttcgcattc
     6241 ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg aaacagaatg cgaagacgaa
     6301 caataaggcc tcccaaatcg gggggccttt tttattgata acaaaaaggc aacactatga
     6361 catcggaaaa cccgttactg gcgctgcgag agaaaatcag cgcgctggat gaaaaattat
     6421 tagcattact ggcagagcgg cgcgaattgg ccgtcgaggt aggaaaagcc aaactgctct
     6481 cgcatcgccc ggtacgagat attgatcgtg aacgcgattt actggaaaga ttaattacgc
     6541 tcggtaaagc gcaccatctg gacgcccatt acattactcg cctgttccag ctcatcattg
     6601 aagattccgt attaactcag caggctttgc tccagcaaca tctcaataaa attaatccgc
     6661 actcagcacg catcgctttt ctcggcccca aaggttctta ttcccatctt gcggcgcgcc
     6721 agtatgctgc ccgtcacttt gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata
     6781 tttttaatca ggtggaaacc ggccaggccg actatgccgt cgtaccgatt gaaaatacca
     6841 gctccggtgc cataaacgac gtttacgatc tgctgcaaca taccagcttg tcgattgttg
     6901 gcgagatgac gttaactatc gaccattgtt tgttggtctc cggcacgact gatttatcca
     6961 ctattaatac ggtctacagc catccgcagc cattccagca atgcagcaaa ttccttaatc
     7021 gttatcctca ctggaagatt gaatataccg aaagtacgtc tgcggcaatg gaaaaggttg
     7081 cacaggcaaa atcaccgcat gttgctgcgt tgggaagcga agctggcggc actttgtacg
     7141 gtttgcaggt actggagcgt attgaagcga atcagcaaca aaacttcacc cgatttgtgg
     7201 tgttggcacg taaagccatt aacgtgtctg accaggttcc ggcgaaaacg acgttgttaa
     7261 tggcgaccgg gcaacaagcc ggtgcgctgg ttgaagcgtt gctggtgctg cgcaaccaca
     7321 atctgattat gacccgtctg gaatcacgcc cgattcacgg taatccatgg gaagagatgt
     7381 tttatctgga tattcaggcc aatcttgaat cagcggaaat gcaaaaagca ttgaaagagt
     7441 taggggaaat tacccgttca atgaaggtat tgggctgtta cccaagtgag aacgtagtgc
     7501 ctgttgatcc aacctgatga aaaggtgccg gatgatgtga atcatccggc actggattat
     7561 tactggcggt tgtcattcgc ctgacgcaat aacacgcggc tttcactctg aaaacgctgt
     7621 gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt caataaacgc ctgcttatcg
     7681 ccctgctcca gcaactcaat cgcctcgccg aaacgcttat agtaacgttt gattaacgcc
     7741 agattacgct ctgacgacat aataatgtcg gcataaagct gcggatcctg agcaaacagt
     7801 cgcccgacca tcgccagctc aaggcggtaa atcggcgaag agagcgccag aagttgctca
     7861 agctgaacat tttcttctgc cagatgcagc ccataagcaa aagtagcaaa gtggcgcaga
     7921 gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc taatacgatg cagccgagcg
     7981 ccccagacct gaatttgctc cagaaaccat tggtatgctt ccggcttacg tccatcacac
     8041 cagaccacaa cttgctttgc caggctaccg ctgtccgggc cgaacatcgg gtgtaacccc
     8101 agtaccgggc catcgtgcgc cgccagcatg gcctgtaatg gtccattttt cactgatgcc
     8161 agatcaacca gaatacaatc tttcggtaaa ggcggtaatt tgccgataac ttgctcagta
     8221 acgtggattg gcacactaac aatcaccatt ccggcatcgg caacaatatc agccgctcga
     8281 tcccagtcat gttgctccag aatccgcacc tgataacccg atagtgtcag catcttctcg
     8341 aacaggcgtc ccatctgacc gccaccgccg acgataacca ccggacgcag tgacggacaa
     8401 agtgttttaa atcctttgtc gttttcactg gagtaagatt cacgcatcac ccgacgcaaa
     8461 acatcctcaa tcagatctgg cggtacaccc agagcttccg cctctgcacg acgcgaggcc
     8521 aacatagatg cctcgcgctc cggaacataa ataggcagtc caaagcggct tttcacctcg
     8581 cccacttcag caaccagttc cagacgcttc gctaataaat tcagcagcgc tttatcgact
     8641 tcatcaattt gatcgcgtaa tgcggtcaat tcagcaacca taataaacct cttaagccac
     8701 gcgagccgtc agctgcccgt tcagatcctg atgaatttca cgcagcaagg catcggtcat
     8761 ttcccagcta atgcaggcat cggttacgga tacaccgtat ttcatttcac tgcgcggttg
     8821 ctcagaagac tgattgccct cgtggatatt actttcgatc atcagaccaa taattgagcg
     8881 attgccatct ttgatttgag caaccacgga ttctgccacc gcaggctgac ggcgataatc
     8941 tttattggaa ttaccgtggc tgcaatctac catcagagac gggcgcagtc ccgcctgttc
     9001 catctctttt tcacattgcg caacatccgc agggctatag ttcggcgctt taccaccgcg
     9061 caggatgaca tgcccgtccg gattcccctg agtttgtagc aacgcaacct gccctgcctg
     9121 gttaatgcca acaaaacggt gcggctgggc ggcggcgcgc atagcgttaa ttgctgttgc
     9181 cagactgccg tcggtgccgt ttttaaaacc aaccggcatg gaaagcccgg aggccatttc
     9241 acggtgagtt tgcgattccg ttgtacgagc accaattgct gaccagctaa acagatcgcc
     9301 caggtattgc gggctattcg gatctaacgc ttccgtcgcc agtggcagtc ccatattcac
     9361 cagctcaagc agcaatttac gcgcgatctg cagcccggct tctacatcaa aagagccatc
     9421 catatgggga tcgttaatta accctttcca gccgacagtg gtacggggtt tttcaaaata
     9481 gacgcgcatt accagataga ggctatcgct gacctctgcg gcaagggctt taaatcgacg
     9541 agcatattcc agagcagttt ccggatcatg aatggaacaa ggaccacata ctaccagcag
     9601 acgaggatcg cgcccggcga taatatctga aatggttcta cgcgagtcag caatctgggc
     9661 ttcttgttgc aggctcaatg gaaaagcggc cttcagttgt tccggagtca ttaaaacctg
     9721 ttcgtcggta atatgtacgt tattcagcgc gtctttttgc atgatggcga tcctgtttat
     9781 gctcgtttgc gatagttgat cctcagcgag gatgacgtaa cgataacaca taaagtaaag
     9841 ttttcaatcc atatttcgta catttttatt tacacaggca atttagtcgc gatttcaacc
     9901 cttacttctg tatagataaa tttacactcc ctttgaaaac aatccgctat gctttgaaaa
     9961 aggagaaaga aatgatgaaa aagtttatcg cccccttgtt ggctttactg gttagcggat
    10021 gtcagattga tccttatact cacgcgccaa ccttgaccag caccgactgg tatgatgttg
    10081 gtatggaaga tgcgatatcg ggcagcgcca taaaagatga cgatgcattt agcgattcac
    10141 aggcggatcg cggtctatac cttaaaggat atgccgaagg acaaaagaaa acttgccaga
    10201 ccgattttac ttatgcccgg ggactttccg gtaaaagctt tcctgccagc tgcaacaatg
    10261 ttgaaaatgc cagccaactg catgaagtat ggcaaaaagg ggctgatgaa aatgccagca
    10321 c
//
LOCUS       DAZGJW010000158        10284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZGJW010000158 DAZGJW010000000
VERSION     DAZGJW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.158"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            303..1073
                     /gene="yjjP"
                     /locus_tag="ACHKIK_004176"
     CDS             303..1073
                     /gene="yjjP"
                     /locus_tag="ACHKIK_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO9085237.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSDTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1064..1537
                     /gene="yjjB"
                     /locus_tag="ACHKIK_004177"
     CDS             1064..1537
                     /gene="yjjB"
                     /locus_tag="ACHKIK_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO9085238.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1644..2183
                     /gene="dnaT"
                     /locus_tag="ACHKIK_004178"
     CDS             1644..2183
                     /gene="dnaT"
                     /locus_tag="ACHKIK_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO9085239.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2186..2923
                     /gene="dnaC"
                     /locus_tag="ACHKIK_004179"
     CDS             2186..2923
                     /gene="dnaC"
                     /locus_tag="ACHKIK_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO9085240.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2972..3466
                     /gene="yjjA"
                     /locus_tag="ACHKIK_004180"
     CDS             2972..3466
                     /gene="yjjA"
                     /locus_tag="ACHKIK_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO9085241.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3720..6011
                     /gene="opgB"
                     /locus_tag="ACHKIK_004181"
     CDS             3720..6011
                     /gene="opgB"
                     /locus_tag="ACHKIK_004181"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO9085242.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGAGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6150..7172)
                     /gene="lgoD"
                     /locus_tag="ACHKIK_004182"
     CDS             complement(6150..7172)
                     /gene="lgoD"
                     /locus_tag="ACHKIK_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFO9085243.1"
                     /translation="MSTMKVLICQEIKNLVWTVRERPVPGDNEALIKIKTVGICGTDI
                     HAWAGNQPFFSYPRVLGHEICGEIIESGKNFHNFNIGQQVAVIPYISCQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7311..8216
                     /locus_tag="ACHKIK_004183"
     CDS             7311..8216
                     /locus_tag="ACHKIK_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO9085244.1"
                     /translation="MSRSQNLRHNVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARKPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
     gene            complement(8387..10039)
                     /locus_tag="ACHKIK_004184"
     CDS             complement(8387..10039)
                     /locus_tag="ACHKIK_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO9085245.1"
                     /translation="MEILLTILILTLVVSLTSVLTRLSPVQIPLPLIQIAAGAVLAQP
                     IFGLHVEFNPELFLLLFIPPLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGY
                     VIHLLLPNVPLIAAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGL
                     VALKFAVAVTMGTMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEP
                     SIQIVLMLLLPFIVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWS
                     MLEYVFNGMVFLILGLQLPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGW
                     LWLMRLYSRKRPGKHPMLFAGYKTRHMLLATFAGVRGAITLAGVLSIPLLLPDGTPFP
                     GRYLLVFLATGVILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSV
                     QKTEQRLLRTSTENLDNELIMETGARVIGYMRRSEDDMSENDNSLHMQRAKLERTFNL
                     AALNARRAAIYQMRARQEVSDETLAKIVSELDVLETLIMDSR"
BASE COUNT         2680 a         2638 c         2441 g         2525 t
ORIGIN      
        1 aaccaatatt tattcaatga aatctgatgg ttttaacttg ttaacattaa aaaacgtgcc
       61 cggttatcta ttatcctgat tcgtaaaggc caaacaataa attcattatg atgataatat
      121 tcatgtgata gtttgtttgc ggcgagagat aattcgcttt ttatcaccga gcttctcccg
      181 ataaccagga ttaatcagcg caattttgat cttaacctac cgcaaaacaa ccgtgcttca
      241 tgatacaaat ggattctcat tatctgaaca atacgcagca cgtctatgac aaagggcgag
      301 ttatgcaaac tgagcaacag cgagccgtaa cacggctttg tatccagtgt ggattatttc
      361 ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct ttcctcacga ctgggtcggg
      421 cgctgggaat ggacagcgtc gaaagttcta tctcttcgaa cgccatagtg ctgacaacca
      481 ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca cgatcgcggc attaatatgc
      541 atgtggtgac tgaagtccag cacattgtga ttcttgcgga gcatcatctg ctggattaca
      601 aaggcgtaga gaaacgattt agccaaattc agccattacg ttacccaaga tggctggtag
      661 ccttaatggt tggcctttct tgcgcctgtt tctgtaaact caataacggt ggctgggatg
      721 gtgccgtcat caccttcttt gccagtacga ccgcgatgta tatccgccag ctgctggcac
      781 aacgtcatct tcatccacag atcaactttt gccttaccgc tttcgccgcc accaccattt
      841 ccggattgct tttgcaactc cccactttca gcgatacacc caccattgcg atggccgcca
      901 gcgttctgct gctggtgccg ggctttccgt tgattaatgc cgtcgccgat atgtttaaag
      961 gccacatcaa taccggactg gcacgctggg cgatcgccag tctgctgacg ctggctacct
     1021 gcgtcggcgt ggtgatggca ctgacgattt gggggctacg cggatgggtg tgatcgaatt
     1081 tctgttagcg ttggcgcagg atatgatcct cgccgccatt cctgcggtcg gctttgcgat
     1141 ggtgttcaac gttcccgttc gggcgttacg ctggtgtgcg ctgcttggcg cgattggtca
     1201 tggttcacga atgatcttaa tgaccagcgg gctgaatatt gagtggtcaa cttttatggc
     1261 ttctatgctg gtcggcacca ttggtattca atggtcgcgc tggtatctgg cgcatccgaa
     1321 agtgtttacc gtggcggccg ttatccctat gttcccgggc atatcggctt ataccgcaat
     1381 gatttcggcg gtaaaaatca gccagttagg ttacagcgaa ccgttgatga ttaccctgtt
     1441 aaccaacttt cttacagctt catcgattgt tggtgcgtta tccatcggtc tttccattcc
     1501 tggattatgg ttgtaccgca agcgccctcg cgtataaaat tgcctcgctt aagcacacgg
     1561 atgagagaca gcctcctctc ctccgtgtgt tactataaaa gttatctccc ttctcgttca
     1621 tcgttccata tttgagaaac agtatgtctt ccagagtttt gaccccggac gtcgttggta
     1681 ttgacgccct ggtacacgat caccaaaccg ttctggcaaa agctgaaggc ggtgtggttg
     1741 ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac gcctgcacgc ctggctgaac
     1801 tgctggcgct ggaagaaaag ctggcgcgtc cgggaagcga tgtcgctctg gacgatcaac
     1861 tctatcagga accgcaagcc gctcccgttg ctgtccccat ggggaaattc gccatgtatc
     1921 cggactggca acccgatgcc gactttatcc gcctggcggc gctatggggc gtggcgctaa
     1981 gagagccggt gaccaccgaa gaactggcct cattcattgc ctactggcag gcggaaggta
     2041 aagtctttca ccatgtgcag tggcaacaaa aactggcgcg cagcctgcaa atcggtcgtg
     2101 ccagcaacgg cggactgccg aaacgagatg tgaatacggt cagcgaacct gacagccaaa
     2161 ttccaccagg attcagaggg taacgatgaa aaacgttggc gacctgatgc aacgcctgca
     2221 aaaaatgatg cctgcccata tcaaacctgc attcaaaacg ggtgaagagc tactggcctg
     2281 gcagaaagaa caaggggcga tccgctccgc cgctctcgaa cgtgaaaatc gggcgatgaa
     2341 aatgcagcgc acctttaacc gctccggtat tcgtccactg catcagaact gctcctttga
     2401 gaactatcgc gttgagtgtg aagggcagat gaatgcgtta agcaaagcgc gccagtatgt
     2461 cgaagagttt gacggcaaca tcgccagctt catcttttct ggtaagccag gaaccggcaa
     2521 aaaccatctg gcggcggcaa tctgcaacga gctgctgcta cgcggtaaat ccgtattgat
     2581 catcaccgtg gccgatatta tgtcggcgat gaaagatacc ttcaggaata gcggtaccag
     2641 cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg ctggtgatcg atgagatcgg
     2701 cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac cagatcgtcg atcgccgctc
     2761 ttcttccaaa cgcccaaccg ggatgctgac caacagtaat atggaagaga tgaccaagtt
     2821 gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac agtttgtggg tgatcttcaa
     2881 ctgggatagc taccgtagcc gggtaacagg taaagagtat taatccccgt ttctggcggt
     2941 atactgcgac acaattccac cttgagtaat gatgatgaaa actgttaaac atcttctgtg
     3001 ctgtgccatt gccgccagcg cattaatttc caccggggtg catgctgcgt cctggaaaga
     3061 tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac agcacgacac aggaaggcgg
     3121 ttggtcgctc gcgtcattaa ctaacttgct tagcagcggg aaccaggcct taagcgcaga
     3181 taacatgaac aacgccgcag gcattctgca atactgcgcg aagcaaaagc tggcgtcggt
     3241 aaccgatgcc gaaaacatca agaaccaggt gctggaaaag ctgggcctga acagtgaaga
     3301 acaaaaagaa gacaccaact atctggatgg cattcagggt ttgctgaaaa ccaaagatgg
     3361 tcagcaactc aatctggata acatcggaac gactccgctg gcagaaaagg tgaaaaccaa
     3421 agcctgcgat ctggtgttaa agcaggggct gaacttcatt tcctgaaccg cgaaacgccg
     3481 cgttttagct gttacccggc tgacgcggct ctctcctgcc cttttccctg ctcctggacg
     3541 gttttccccc tgttacacgg gaagcgaccg cgatcaaaaa agtctgttta taaactaatt
     3601 cctaacaaca tcgcattttt ttgacactag aattgcagca atatggcgac gccattacat
     3661 tgtgcttctc aaaaagctta aacgttggct aaacggcccg gtttagtgag gatcgcccgt
     3721 tgtcagaact actctctttc gccctttttc tcgcctctgt gctgatttac gcatggaaag
     3781 cgggacgtaa cacctggtgg tttgcagcca cattaacggt gctggggcta tttgtcgttt
     3841 taaatatcac cctgtttgcc agcgactatt ttactggcgc tggtattaac gacgcggttc
     3901 tctatacctt aaccaacagc ctgaccggtg ctggcgtcag caaatacatc ctgccgggta
     3961 tcggcattgt gctggggctg acagcggtgt tcggtgcgct gggctggatc ctgcgccgtc
     4021 gtcgccatca tccgcaccat tttggttaca gcctgctggc gctcttactg gcgctgggtt
     4081 cggtggacgc cagcccggca tttcgtcaga taacggaact ggtgaaatcc cagtcacgcg
     4141 atggcgaccc ggactttgcg gcttattata aagagccgtc gaaaactatc cctgacccga
     4201 aactcaacct ggtttatatc tacggcgaaa gtctcgagcg gacctatttt gataacgaag
     4261 ctttcccgga tctcacgcct gaactgggcg cgttgaaaaa tgaaggcctg gatttcagcc
     4321 acacgcagca gctgccggga acagattaca cgattgcggg catggtggct tctcagtgcg
     4381 gcattccgct ttttgcccca tttgaaggta acgcctccgc ttctgtttcc agcttcttcc
     4441 cgcagaacat ctgtctgggc gatatcctga aaaactcggg ttatcagaac tatttcgtgc
     4501 agggcgcgaa tctgcgtttt gccggtaaag atgtgttcct gaagtcgcat ggcttcgacc
     4561 acttatacgg ctcagaagag ctgaaaagcg tggtagccga cccgcactat cgcaacgact
     4621 ggggattcta cgacgatacc gttctcgatg aagcgtggaa aaagtttgaa gagctttccc
     4681 gctcaggtca gcgattctca ctgtttaccc tgacagtcga tacccatcac ccggatggtt
     4741 ttatctcgcg tacctgtaac cgcaaaaaat atgattttga cggtaagccg aaccagtcat
     4801 tcagcgcggt aagttgcagc caggagaata tcgcggcgtt tatcaacaaa atcaaagcgt
     4861 caccgtggtt taaagatact gtcatcgtcg tctcttctga ccatttagcg atgaacaaca
     4921 cggcgtggaa atacctcaat aagcaggatc gcaataacct gttttttgtc attcgtggcg
     4981 acaagccgca gcaagagacg ctggcagtga agcgtaacac gatggataac ggcgcgacgg
     5041 tgctggacat tctcggtggc gataactatc tcggacttgg tcgtagcagt ttatccgggc
     5101 agtcaatgtc ggaaatcttc ctcaatatca aagagaaaac attggcgtgg aagccggata
     5161 tcatccgcct gtggaaattc cctaaagaga tgaaagagtt caccatcgac cagcagaaaa
     5221 acatgattgc cttctcgggt agccatttcc gtttgccgct gctgttgcgg gtttcagaca
     5281 aacgcgtgga accgctgccg gaaagcgaat actcagcacc gctgcgtttc cagctggccg
     5341 atttcgctcc acgcgacaat ttcgtctggg ttgaccgttg ctacaagatg gcacaactct
     5401 gggctccgga actggcactt tccaccgact ggtgtgtctc gcaggggcag cttggcggtc
     5461 agcaaattgt tcagcatgtt gacaaaacaa catggcaggg caaaacggca tttaaagata
     5521 cggtcatcga catggcgcgt tacaaaggca atgtcgatac gctgaagatt gttgataacg
     5581 atattcgcta caaagccgac agtttcatct tcaacgtcgc cggtgcgcca gaagaggtga
     5641 aacagtttag tgggatttcc cgtccagagt cgtggggccg ctggtccaac gcgcagctgg
     5701 gcgatgaagt aaaaatcgag tacaagcatc cgctgccgaa gaaatttgac ctggtgatta
     5761 ccgccaaagc atacggcaat aacgccagcc gtcctattcc ggtacgcgta ggcaatgaag
     5821 aacaaaccct tgtgctgggc aatgaagtga ccaccaccac actgcatttc gataacccaa
     5881 ccgatgccga cacactggta attgtgccgc cggaacctgt ctcaaccaac gaagggaata
     5941 tcctcggaca ctcgccgcgt aagctcggga tcggcatggt ggaaattaaa gttgtggaac
     6001 gtgaagggta attcctgact gatgtgtagg tcgcataagg tgcgacctac acaccttctc
     6061 cggcctacaa aacatcattg agaaacagaa aaaaacagcg cccaacactt ccccggacgc
     6121 tgcatggcaa ctttaataat ctcgccttat cagaaagtaa ttacaccttt aattaactca
     6181 cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg tggcgaacgg atagcgatgg
     6241 gttaacatca tgtcagcagt gatttttcct tccgccatca gtcgaccgac tttagcaaaa
     6301 tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt cttttttatg gaattccgga
     6361 tcggagaact gcaactcacc tttaaacagg ccgacaaata ccaccgtgcc gccgtgacga
     6421 atcagattca cggtgttatt catcgcatgt tgattacctg tcgcgtcgat cactttctgc
     6481 gccagcgaac caccaaactg cgcccgcaac tgcgcgtcaa aatcttcggc tgacgggtcc
     6541 agcacgggca actccagacg cgttgccaca tgttcacggc gcgccggact ggtatccgcc
     6601 accaccacct gtgcgccatc ggctttagcg attgccgccg cgcccagacc gattggcccc
     6661 gccccgacca ccagcacctg ctcgccggga gcaatggctg cgcgacgcac cgcatgagcg
     6721 ctaatagcga aaggttcaat caatgcggcc gcctgcgggt caataccgtc tgcgggcaaa
     6781 atgttcgcca ccggcacgct caaatactca ctaaaaccgc catcctgatg cacgccaatg
     6841 actgaaattt tttcacagca attggtacgc ccgcttttac acgccgggca ttgctgacaa
     6901 gaaatatacg gaattaccgc aacttgctgt ccgatattaa aattgtgaaa gttttttcct
     6961 gattcaataa tctccccaca tatttcatgg cctaaaacgc gtggatagct aaaaaatggt
     7021 tgattgcccg cccaggcatg aatatctgta ccgcaaatcc cgacggtttt aattttaatt
     7081 aatgcttcat tatcgcctgg caccggtctt tcccggactg tccatactaa attctttatt
     7141 tcctgacaaa ttaatacttt cattgtggac atgattcacc gctcctttta ttttatgact
     7201 ttcagatatc gtcgaattta taagagcacc tgaatttacc cataaaatat gcggatgatc
     7261 acgcataaaa atgttttaaa tcggttttta atgccggact ggagggaaga atgagtcgtt
     7321 cgcagaattt acgtcataac gtcataaatc aggtgattga agatatggcg aggggcaata
     7381 ttccttctcc gctgccttca caaagtgggc tggcagaaat gtataacatc agtcgcacta
     7441 cggtacgcca catactccag catttaagcg catgtggcgt gctcaccctg gtggggaaaa
     7501 attatgttat cgcccgcaaa cccgaacaga acgatggatt tgattgcatc acggcctcgc
     7561 tgaaagagca aaaccgtctt ttcgagcagg catttttcac catgattaac ctgcgccagt
     7621 tacgcgcggg agaaagcttt tccgaacttc agctggcgcg agcggcaggc gtcagtccgg
     7681 tagtagtacg agaatatctt ttgaaattcg gtcgttataa tcttattcaa agcgaaaagc
     7741 gcggccagtg gagcatgaag cagttcgatc aatcctacgc cgagcagctg tttgagctac
     7801 gcgaaatgct cgaaacacat tccctgcaac actttctcaa ccttcccgat gacgatcccc
     7861 gctggttgca ggcgaaaacc atgctcgaac gtcacaggac attgcgcgac agcattggca
     7921 gcaactttcg gatgttttct caattagacc gtgattttca tgccatgctg ttatccgctg
     7981 ctgacaacat cttttttaat caatcacttg aaattatctc ggtgatcttt cattttcact
     8041 atcaatggga tgaaagcgat ctcaagcagc gcaatatcat tgctgttgat gagcatatga
     8101 ccattctcag cgccctgatt tgccgtagcg atttagatgc aataacggca ttgcgaaacc
     8161 atctggatac cgccaaacaa tcaatgattc gttccatcag gcaacatcac cgataaatta
     8221 agtacagatt aaaaacattg attcttggtt tatatcacta tgttgtatta atgcagtatt
     8281 aatccaggcg attgttctgc ccataaggaa atacataggg aataaacgat gctggaagta
     8341 ttactctcag ccatcattta actccctatt actggtagcg ataaaattat cggctatcca
     8401 tgattaacgt ttccagcaca tctaattcac tgactatttt tgccagtgtt tcatcactga
     8461 cttcttgccg tgcacgcatc tggtaaatag ccgcacgcct ggcatttaac gctgccagat
     8521 taaatgttct ttccagcttc gcgcgctgca tatgtaaaga attatcattt tccgacatat
     8581 catcttcgct acgccgcata taaccgataa ctctggcccc tgtttccata atcaactcgt
     8641 tgtcgaggtt ttctgtactg gtgcgtagca aacgttgctc agtcttttgc acactgagta
     8701 tggccatttt ggccataaac agatcggcag cttcctcttc tttttgccgg ttatcgctga
     8761 aaatgcccac atttttcccc agtagtaacg gcagaataat aatgccgcaa accagcgaaa
     8821 agaggatcac acctgtggcg agaaatacca gtaagtaacg cccgggaaaa ggtgtgccat
     8881 ccggcagcaa taacgggata gaaagcaccc ccgcaagcgt gatggctccg cgcacccccg
     8941 caaaggtcgc cagcagcata tgacgcgttt tatatccagc aaacagcatc ggatgtttgc
     9001 cgggtcgttt acggctatac agacgcatca gccaaagcca gcccaggcgc acaaccatta
     9061 acgccaggta gatccaaatg acatcaagga gcagcgatcc ggtgtctacc agtggatcgt
     9121 tttctgcacg gatcagtgat tccgaaataa tgcccggcag ttgtaagcct aaaatcagaa
     9181 acaccatgcc gttaaagaca tattcaagca tcgaccagct acttttagct ttaattctca
     9241 ggcggatagg cgcgtggtgg actacgccaa tgcgactggt tgttaacccc gcggcgacag
     9301 cggcaagaat gccagataag ccaaaatgtt ccgcaaccag ataaacaata aacggcaaca
     9361 gaagcatcaa tacaatctga atggaaggtt catcgtgagc atatcgactg atcaacagca
     9421 gccctttgcc atataaccag gtaacagcaa tccccacggc aataccgccc aacgcaacga
     9481 caaaaaacgc aatggttgcg ccatgaacgc tgaattccat cgttcccatc gtcactgcaa
     9541 cagcaaattt caacgccacc aggccggaag catcattcat caacgcttcg ccttcgagca
     9601 cttcctggat gtttttgctg atacgccctt tcccgacaat ccccagcaac gccacggcat
     9661 cggtaggcga caacacggcg gctaatgcga aagcagcgat gagcggcaca ttcggtaaga
     9721 gtaaatgaat cacatagccg acaccaaaaa tagtgattag caccaacaca agcgccaggc
     9781 tgataatttc ccgactgtgc ttgatcaatt ctttaggttg tatttttgag ctttcagcaa
     9841 acaacaaggg gggtataaac aataagagaa aaagttcagg attgaattcc acatgcagac
     9901 caaatatggg ctgggccagt acagcgccag cggcaatctg gatcaaagga agtggtatct
     9961 gaacaggaga cagacgcgta agcacgctgg ttaatgatac taccaacgta agaattaaaa
    10021 ttgttaaaag tatttccata taataaacag gcttcctcat tcataaataa tgggtattat
    10081 taatttaact tcataaaaag cgccctggtt agcacttaac gttaatcatt aatcacagcg
    10141 ttcactcaca tgctcaggag gaaaccagtg agatgagttt tccagttgat aacaaattca
    10201 ccatgtgaat atttactacc tttaaccgtt gtaacgaatc atccgttact ctttgatctc
    10261 cttgccgaac aagttattta gtga
//
LOCUS       DAZGJW010000159        10277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZGJW010000159 DAZGJW010000000
VERSION     DAZGJW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.159"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..259)
                     /locus_tag="ACHKIK_004185"
     CDS             complement(<1..259)
                     /locus_tag="ACHKIK_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9085246.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEH"
     gene            complement(291..1307)
                     /gene="lplA"
                     /locus_tag="ACHKIK_004186"
     CDS             complement(291..1307)
                     /gene="lplA"
                     /locus_tag="ACHKIK_004186"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO9085247.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEVITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            complement(1335..1979)
                     /locus_tag="ACHKIK_004187"
     CDS             complement(1335..1979)
                     /locus_tag="ACHKIK_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO9085248.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            2085..3053
                     /gene="serB"
                     /locus_tag="ACHKIK_004188"
     CDS             2085..3053
                     /gene="serB"
                     /locus_tag="ACHKIK_004188"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO9085249.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            3102..4484
                     /gene="radA"
                     /locus_tag="ACHKIK_004189"
     CDS             3102..4484
                     /gene="radA"
                     /locus_tag="ACHKIK_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO9085250.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            4505..5737
                     /gene="nadR"
                     /locus_tag="ACHKIK_004190"
     CDS             4505..5737
                     /gene="nadR"
                     /locus_tag="ACHKIK_004190"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO9085251.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(6044..7711)
                     /gene="ettA"
                     /locus_tag="ACHKIK_004191"
     CDS             complement(6044..7711)
                     /gene="ettA"
                     /locus_tag="ACHKIK_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO9085252.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            7922..9859
                     /gene="sltY"
                     /locus_tag="ACHKIK_004192"
     CDS             7922..9859
                     /gene="sltY"
                     /locus_tag="ACHKIK_004192"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO9085253.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     HIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSTTEWGRRY"
     gene            9949..10275
                     /gene="trpR"
                     /locus_tag="ACHKIK_004193"
     CDS             9949..10275
                     /gene="trpR"
                     /locus_tag="ACHKIK_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO9085254.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
BASE COUNT         2314 a         2661 c         2920 g         2382 t
ORIGIN      
        1 tatgttcagc cgtcatccag gaagggcata tcagcaactc tggtggacaa ccataaattt
       61 ctgccatttt gcttttgatg tttgagctaa gccgttccgt cttttcgatc tgttttagag
      121 aattaatcga gatacctaac attttactta cttcagcaag cgataattta tgcctgatac
      181 gcacggcggc tagccaggaa atacgctggc ttaacattaa attgataatc tcccgctcct
      241 ggttttccag atcgatcata ttcccttcct ttggtatttt aatcagcgag ttaccttacc
      301 gcccccgcta tccacgtcga caactcccgt agctcttttt cctggtccgg gaagtcaacc
      361 aacagcgctt cgcactcttg ttgcagcata tccgcgcggt acaggcagcc ttgcagtcgc
      421 ccggcgaggg cttccagcgg cgcggggttg aggctgtcgg taaacacctg ggcgcgggtg
      481 atatggcctt tttcaacgtc gaaatgcagt tccacgccgc cccagctaaa gcgttcatcc
      541 agcagatgcg agaatgccgg agcctgaccg aagttccatt cccagctact ctgacgggca
      601 aaggtttcgg cgaagtttgg caagtctggc gttttgtccg gggagatgat ttccgcttct
      661 acacgctcgc cataatgggc gaaaaaggcc ttggttatga cctcgcaaac ctgctcatgg
      721 gtgatccccg gcagcagctc ggtgaggttg gtcacgcggg aacgcactga ggtaatgcct
      781 ttcgccgcca gtttcttttt atccggattg agatagtttg ccaggcggct aaggtcggca
      841 ttgagcagca aagtgccgtg gtggaagcca cgatctttgg tttcgcgata ggccgagcct
      901 gagactttgc ggtcgccttc gactgttttc accaccagat cgttacgccc ggacgcttcg
      961 gcgctgacgc cgagcgcgtt cagcgcattg agcacaatcg acgtagagat ggttttatcg
     1021 tactccggct tgccagccat aaaggtaaag caggtattgc cgagatcgtg gaacaccgcg
     1081 ccgccaccgc tactgcgccg cgccaggcgg acgttatctt cttccatccg ccgggtatta
     1141 cactctttcc acgggttctg cgcgcgacca atcactaccg tgtcggcatt gcgccagaga
     1201 aacagaacgc gctgcgtggc gggcatttgg cgaaaaatac actcttccac cgccaggtta
     1261 aaccacgggt cgtaagagtc agagatgagc aggcgtaatg tggacataac gatttccttt
     1321 cttgtagtaa tgggtcactc ttttttctcg ctttcttcct cttccggcac cggtttgctg
     1381 gcggttaaca ggaagggcga ttgctgccag cgggtgcgtt taccctgtag cagcgtgcgg
     1441 gtcagcacta cgccgattgc cagtgagagc agcaacatca ggcgtaaaat gttagtggtg
     1501 ttatccacct gttgggcttc ggtggcgagc gtatgggtgt cgagtgtcag gcgcagatag
     1561 ccgagcggtc cgtttttacc cgcaattggc tcgacaatct gctggttaaa atagccgcct
     1621 gcttttttac cgtcgagcgc cagccggtcg cgcacttcga cgctttcgcc tgaacgggcg
     1681 ataagatcgc cttgttcgtc atacacaccc gcgtcgagga tgcggctttc atccgttaac
     1741 tgatcgagga tcgcctgaat gcgtttttca tctggtgagt cggtacgcat cagcggtgca
     1801 acgttcagcg tcacctgacg cgccagggtg cgggccagtt cttccagctg tggattacgc
     1861 tgtcgctggt ggttttgact aaaccatgac gccccctgca tcagtgccac taacaaggca
     1921 agacagaaca ggacaatcac tgcccgatgc agccggaatt tcagttttgt gcgagccatc
     1981 ttccaccctt tgaaaatttg agacttaatg ttgccagaag caatggatac aaggtagcct
     2041 catgcgttat tttccctgct tcgaacgatt ttacaggagc cttaatgcct aacattacct
     2101 ggtgcgacct gcctgaagat gtctctttat ggccgggtct gcctctttca ttaagtggtg
     2161 atgaagtgat gccactggat taccacgcag gtcgtagcgg ctggctgctg tatggtcgtg
     2221 ggctggataa acaacgcctg acccaatacc agagcaaact gggcgcggcg atggtgattg
     2281 ttgccgcctg gtgcgtggaa gattatcagg tgattcgtct ggcaggttca ctcaccgcgc
     2341 gcgctacgcg cctggcccat gaagcgcaac tggatgtcgc gccgctgggg aaaatcccgc
     2401 acctgcgcac gccgggtttg ctggtgatgg acatggattc caccgccatc cagattgaat
     2461 gtattgatga aattgccaaa ctggccggaa cgggcgagat ggtggcggag gtaaccgaac
     2521 gggcgatgcg cggcgaactc gattttaccg ccagcctgcg cagccgcgtg gcgacgctga
     2581 aaggcgctga tgccaatatt ctgcaacagg tgcgtgaaaa tctgccgctg atgccaggct
     2641 taacgcagct ggtgctcaag ctggaaacgc tgggctggaa agtggcgatt gcctccggcg
     2701 gctttacttt ctttgctgaa tacctgcgcg acaagctgcg cctgaccgcc gtggtagcca
     2761 atgaactgga gatcatggac ggtaaattta ccggcaatgt gatcggcgac atcgttgacg
     2821 cgcagtacaa agcgaaaact ctgacccgcc tcgcgcagga gtatgaaatc ccgctggcgc
     2881 agaccgtggc gattggcgat ggagccaatg acctgccgat gatcaaagcg gcagggctgg
     2941 ggattgccta ccatgccaag ccaaaagtga atgaaaagac ggaagtcacc atccgtcacg
     3001 ctgacctgat gggggtattc tgcatcctct ctggcagcct gaatcagaag taattgcctg
     3061 cccgtcatcc tgcgggcggc acagcattaa cgaggtacac cgtggcaaaa gctccaaaac
     3121 gcgcctttgt ttgtaatgaa tgcggggccg attatccgcg ctggcagggg cagtgcagtg
     3181 cctgtcatgc ctggaacacc atcaccgagg tgcgtcttgc tgcgtcgcca acggtggcgc
     3241 gtaacgagcg tctcagtggc tatgccggta gcgccggggt ggcaaaagtc cagaaactct
     3301 ccgacatcag ccttgaagag ctgccgcgtt tttccaccgg atttaaagag ttcgaccgcg
     3361 tattaggcgg cggcgtggta ccgggaagtg ccattctgat tggcggtaac ccaggcgcgg
     3421 ggaaatccac gctgttgctg caaacgctgt gcaaactggc ccagcagatg aaaacgctgt
     3481 atgtcaccgg cgaagagtcg ctgcaacagg tggcaatgcg cgctcatcgc cttggcctgc
     3541 cgactgacaa tctcaatatg ttgtcggaaa ccagcatcga gcagatctgc ctgattgctg
     3601 aagaagagca accgaagctg atggtaattg actccatcca ggtgatgcat atggcggatg
     3661 tacagtcatc gcctggtagc gtggcgcagg tgcgtgaaac ggcggcttat ttgacacgct
     3721 tcgccaaaac gcgcggtgtg gcgattgtca tggttggaca cgtaaccaaa gatggctcgc
     3781 tggcgggccc taaagtgctg gaacactgta tcgactgttc ggtgcttttg gatggcgatg
     3841 ccgactcccg ttttcgcacc ttgcgcagcc ataaaaaccg cttcggcgcg gtgaatgagc
     3901 tgggcgtctt cgcgatgacc gaacaggggc tgcgtgaagt cagcaaccct tcggcaattt
     3961 tcttaagtcg cggagatgaa gtgacctccg gcagctccgt gatggtggtg tgggaaggaa
     4021 cgcgtccgtt gctggtggag attcaggcgc tggtcgatca ctcgatgatg gcgaatccgc
     4081 gccgcgtggc ggtaggtctg gagcaaaacc gtctggcaat cctgctggct gtcttgcacc
     4141 gtcacggtgg tctgcaaatg gccgatcagg atgtatttgt gaacgtggtc ggtggtgtga
     4201 aggtaaccga aaccagtgcc gacttagcgt tactgctggc gatggtttcc agcctgcgtg
     4261 acagaccgct accgcaggat ctggtggtgt ttggtgaagt cgggctggca ggggagatcc
     4321 gcccggtgcc cagcggtcag gagcgaatct ctgaagcggc gaaacacggt tttcgccggg
     4381 cgattgttcc ggcggctaac gtgccgaaaa aagcgccgga agggatgcag atttttggcg
     4441 ttaaaaaact ctccgacgcg cttagcgtgt tcgacgactt ataatgagag atacggaggg
     4501 agatatgtcg tcatttgatt acctgaaaac tgccatcaag caacagggct gcacgttaca
     4561 gcaggtagct gatgccagcg gtatgaccaa agggtattta agccagttac tgaatgccaa
     4621 aattaaaagc cccagcgcgc aaaagctgga ggcgttgcac cgttttttgg ggcttgaatt
     4681 tccccggcag aagaaaacca ttggtgtcgt attcggtaag ttctacccgc tgcataccgg
     4741 acatatctac cttatccagc gcgcctgtag ccaggttgac gagctgcata tcattatggg
     4801 ttttgacgat acccgcgacc gcgcgttgtt tgaagacagc gccatgtcgc agcagcccac
     4861 ggtgccggat cgtctgcgct ggttattgca aacttttaaa tatcagaaaa atattcgcat
     4921 tcatgctttc aacgaagagg gcatggagcc gtatccgcat ggctgggatg tgtggagcaa
     4981 cggcatcaaa aagtttatgg ctgaaaaggg gatccagccg gacctgatct acacctcgga
     5041 agaagccgat gcgccacagt atatggaaca tctggggatc gagacggtac tggtcgatcc
     5101 gaaacgtacc tttatgagta tcagcggtgc gcagatccgc gaaaacccgt tccgctactg
     5161 ggaatatatt cctacggaag tgaagccgtt ctttgtgcgt accgtggcga tccttggcgg
     5221 cgagtcgagc ggtaaatcca ccctggtaaa caaacttgcc aatatcttca ataccaccag
     5281 tgcgtgggaa tatggtcgcg attatgtctt ttcacacctc ggcggtgatg agatcgcatt
     5341 gcagtattcc gactacgata aaatcgcgct gggccacgca caatacattg attttgcagt
     5401 gaaatacgcc aataaagtgg cgtttattga cactgatttt gtcactaccc aggcgttctg
     5461 caaaaagtac gaagggcgtg agcatccgtt cgtgcaggcg ttgattgatg aataccgttt
     5521 cgatctggtg atcctgctgg agaacaacac gccgtgggtg gcggatggtt tacgcagcct
     5581 cggcagttcg gtggatcgca aagagttcca gaacttgctg gtggagatgc tggaagagaa
     5641 caatatcgaa ttcgtgcggg ttgaagagga cgattatgac agccgtttcc tgcgctgcgt
     5701 ggagctggtg cgggagatga tgggggagca gagataaccg cgatgaaacg gctcaaaggc
     5761 gaggtataaa ataagttttt tgatgagttg aaaacctttc tggaagaggc tgtcgagatt
     5821 aaacaaggtt tgaaaaaacc tgcacgggtg acccgctacg aaattgcgga tactaaggct
     5881 gttgtaggcc ggaaaaggcg ttcacgccgt atccggcatt cggtgctcaa tgcctgatgc
     5941 gacgcttacc gcgtcttatc aggcctggag attcacaacg tattgaaatt tcttggtttg
     6001 taggccggat aaggcgttca cgccgcatcc ggcattttac gcattacttc gcaatacgct
     6061 tgtacttgat acgcttcggc tccagtgcgt ctgcgcccag cgtgcgtttc ttgtactctt
     6121 cgtactcagt aaagttacct tcgaagaact caactttacc ttcatcctgg tagtccagga
     6181 tgtgcgtggc gatacggtcg aggaaccaac ggtcgtgcga gataaccatc gcacagcccg
     6241 ggaactccag cagggcgttt tccagcgcgc gcagggtttc gatatccagg tcgttggttg
     6301 gttcgtcgag cagcagcatg ttgccgccaa cctgcagcag cttcgccaga tgcagacgac
     6361 cgcgctcacc accggaaagt tcaccaacgc gtttaccctg atcaacccct ttaaagttaa
     6421 agcggccaac gtaggcgcgg cttggcatct cggtgttgcc gatcttcata atatccagcc
     6481 cgccggaaac ttcttcccaa acggttttgc tgttatccat tgagtcacgg aactgatcaa
     6541 ccgatgccag tttcaccgtt tcacctaaag tgatggtgcc gctgtccggc tgttcctgac
     6601 cagagatcat acggaacagg gtcgatttac ccgcgccgtt cggaccgatg atcccgacga
     6661 ttgccccttt cgggatcgag aagctcaggt catcaatcag caggcgatca ccgtaggatt
     6721 tacgcaggtt gctgacttcc agcactttat cgcccagacg cggtccaggt ggaataaaca
     6781 gttcgttggt ttcgttacgt ttctgatatt cggtgctgtt cagctcttca aagcgtgcca
     6841 gacgtgcttt acctttcgac tggcggcctt tagttccctg acgtacccac tccagctctt
     6901 tctcgatcga cttacgacgc gccgcttctt gtgaagcttc ctgcgccagg cgctgatctt
     6961 tctgctccag ccaggaggag tagttgcctt cccacggaat accttcaccg cggtcaagtt
     7021 cgaggatcca gcctgcaacg ttatcgagga agtaacggtc gtgggtaatc gccaccacgg
     7081 tgccctcgaa gtcgtgcagg aagcgttcca gccaggccac ggattcggca tccaggtggt
     7141 tggtcggttc gtcgagcagc agcatgtctg gtttttccag cagcaggcgg cacaacgcta
     7201 cgcgacgacg ctcaccaccg gagaggttag cgattttcgc gtcccagtcc ggcagacgta
     7261 gcgcatccgc cgcacgctcc agctgtacgt tcaggttatg accgtcgtga gcctgaatga
     7321 tctcttccag acggccttgt tcagcggcca gcttgtcaaa atcggcatcc ggatcggcgt
     7381 acagcgcata cacttcatcc aggcgtttca gggcgttaac cacttcagaa accgcttctt
     7441 caatggactc acgcacggtg tgttccgggt tcagctgcgg ttcctgcggc aggtaaccaa
     7501 tcttgatgtc tggctgcgga cgcgcttcac cttcgatgtc tttatcaatg cccgccataa
     7561 tgcgcagcag ggtagactta cccgcgccgt tcagacccag gacaccaatt tttgccccag
     7621 ggaagaaact cagagagatg tttttcaaaa tatgacgttt cggcggaaca actttgccga
     7681 cacgatgcat ggtataaacg aattgagcca cgttggactt cgcctctatg tttatcgtga
     7741 tgatgagttt tcaaaggcga agtgtagcct ttttccccgc ctaatcccag ttggtcgatc
     7801 accttcgcag taaaagtaaa aaagtgtccg taacgtggcg taaacggcaa tgactggtta
     7861 gcataaatct attacgcggc atgacgctgc attgatgtat ttacacttag aggatgcgct
     7921 tgtggaaaaa gccaaacaag ttacctggcg gctgttggct gccggtgtct gtctgctgac
     7981 ggtcagcagc gtggcgcgag ccgactcact ggatgagcag cgtagccgtt acgcgcaaat
     8041 caagcaggcc tgggataatc gacaaatgga tgtggtcgaa caaatgatgc ctggactgaa
     8101 ggattatccg ctttatccct acctggaata ccgccagatc accgatgatc tgatgaatca
     8161 accggcggtg acggtcacta actttgttcg cgctaacccc acgcttcctc ccgctcgcac
     8221 gctgcaatct cgtttcgtca atgaactggc gcggcgtgaa gactggcgtg gcttgttagc
     8281 ctttagcccg gaaaagcccg gaactaccga agcgcaatgt aattactact atgcgaaatg
     8341 gaacaccggg cagagtgaag aagcctggca aggggcgaaa gagctgtggc taaccggcaa
     8401 gagccagcct aacgcctgtg acaagttatt tagcgtctgg cgtgcgtcag gtaaacaaga
     8461 tccgctggct tatttagagc gtatccgtct ggcgatgaaa gcgggtaaca ctggcctggt
     8521 aacagtgctg gcagggcaga tgcctgccga ttaccagact atcgcctcgg caatcatttc
     8581 actggcgaac aaccctaata cggtactgac cttcgcgcgt acaaccggag cgaccgattt
     8641 tacccgtcaa atggcggcgg tggcgtttgc cagtgtggcg cggcaggatg ctgaaaatgc
     8701 acggctgatg atcccgtcgc ttgcccaggc gcagcagctt aatgaagatc atattcagga
     8761 gctgcgcgat atcgtcgcct ggcgtttgat gggcaacgat gtcaccgacg agcaggcgaa
     8821 atggcgcgat gacgccatta tgcgctcgca atccacgtcg ttgattgaac gccgtgtgcg
     8881 aatggcactt ggcaccggcg atcgtcgcgg cctgaatacc tggctggcgc gtctgccgat
     8941 ggaagcaaaa gagaaagatg aatggcgtta ctggcaggcg gatttattgc tggaacgcgg
     9001 acgtgaagct gaagcgaaag agattttgca tcaactcatg caacagcgtg gtttctaccc
     9061 gatggttgca gcacaacgca tcggagaaga gtatgagctg aagattgata aagcgccgca
     9121 gaatgttgac agcgccctga ctcaggggcc ggagatggcg cgcgtgcgcg agttgatgta
     9181 ctggaatctc gataacaccg cgcgtagcga gtgggccaat ctggtgaaga gcaagtcaaa
     9241 aacagagcag gctcaactgg ctcggtatgc tttcaataac caatggtggg atcttagcgt
     9301 tcaggcaacg atcgccggga agctttggga tcatctggaa gagcgattcc cgctggctta
     9361 caacgatctt ttcaaacgtt acaccagtgg gaaagagatc ccgcaaagct atgcgatggc
     9421 gattgcccgt caggagagcg cctggaatcc gaaagtgaaa tcaccggtag gggccagcgg
     9481 cctgatgcag attatgcctg gtacagcgac ccatacggtg aagatgttct ctattcctgg
     9541 ttatagcagc cctgggcaat tgctggatcc ggaaacgaat atcaacattg gcaccagtta
     9601 cctgcaatat gtttatcagc agtttggcaa taaccgtatt ttctcctcag cagcttataa
     9661 cgccggacct ggacgggtgc gaacctggct tggcaacagc gccgggcgta tcgacgctgt
     9721 ggcatttgtc gagagtattc cgttctccga gacgcgcggt tatgtgaaga acgtgctggc
     9781 ttatgacgct tactaccgct atttcatggg ggataaaccg acgttgatga gcaccacgga
     9841 atggggacgt cgttactgat ccgcacgttt atgatatgct atcgtactct ttagcgagta
     9901 caaccggggg aggcattttg cttcccccgc taacaatggc gacatattat ggcccaacaa
     9961 tcaccctatt cagcagcgat ggcagaacag cgtcaccagg agtggttacg ttttgtcgac
    10021 ctgcttaaga atgcctacca aaacgatctc catttaccgt tgttaaacct gatgctgacg
    10081 ccagatgagc gcgaagcgtt ggggactcgc gtgcgtattg tcgaagagct gttgcgcggc
    10141 gaaatgagcc agcgtgagtt aaaaaatgaa ctcggcgcgg gcatcgcgac gattacgcgt
    10201 ggatctaaca gcctgaaagc cgcgcccgtt gagctgcgcc agtggctgga agaggtgttg
    10261 ctgaaaagcg attgatt
//
LOCUS       DAZGJW010000160        10267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZGJW010000160 DAZGJW010000000
VERSION     DAZGJW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.160"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            107..1501
                     /gene="galP"
                     /locus_tag="ACHKIK_004194"
     CDS             107..1501
                     /gene="galP"
                     /locus_tag="ACHKIK_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO9085255.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1578..2075
                     /locus_tag="ACHKIK_004195"
     CDS             1578..2075
                     /locus_tag="ACHKIK_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO9085256.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2170..2877
                     /gene="endA"
                     /locus_tag="ACHKIK_004196"
     CDS             2170..2877
                     /gene="endA"
                     /locus_tag="ACHKIK_004196"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO9085257.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2957..3688
                     /gene="rsmE"
                     /locus_tag="ACHKIK_004197"
     CDS             2957..3688
                     /gene="rsmE"
                     /locus_tag="ACHKIK_004197"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO9085258.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3701..4651
                     /gene="gshB"
                     /locus_tag="ACHKIK_004198"
     CDS             3701..4651
                     /gene="gshB"
                     /locus_tag="ACHKIK_004198"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO9085259.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4760..5323
                     /locus_tag="ACHKIK_004199"
     CDS             4760..5323
                     /locus_tag="ACHKIK_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO9085260.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5323..5739
                     /gene="ruvX"
                     /locus_tag="ACHKIK_004200"
     CDS             5323..5739
                     /gene="ruvX"
                     /locus_tag="ACHKIK_004200"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO9085261.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5915..6895)
                     /locus_tag="ACHKIK_004201"
     CDS             complement(5915..6895)
                     /locus_tag="ACHKIK_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO9085262.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWSARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLYQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            6913..7617
                     /gene="yggS"
                     /locus_tag="ACHKIK_004202"
     CDS             6913..7617
                     /gene="yggS"
                     /locus_tag="ACHKIK_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO9085263.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7635..8201
                     /gene="yggT"
                     /locus_tag="ACHKIK_004203"
     CDS             7635..8201
                     /gene="yggT"
                     /locus_tag="ACHKIK_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO9085264.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8198..8488
                     /gene="yggU"
                     /locus_tag="ACHKIK_004204"
     CDS             8198..8488
                     /gene="yggU"
                     /locus_tag="ACHKIK_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO9085265.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8496..9089
                     /locus_tag="ACHKIK_004205"
     CDS             8496..9089
                     /locus_tag="ACHKIK_004205"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO9085266.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTHEEKSAISHRGQALKLLLDALRNG"
     gene            9082..10218
                     /gene="hemW"
                     /locus_tag="ACHKIK_004206"
     CDS             9082..10218
                     /gene="hemW"
                     /locus_tag="ACHKIK_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO9085267.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKSSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2400 a         2725 c         2719 g         2423 t
ORIGIN      
        1 aaatgttagt gtaagcgatt acactgatgt gatttgcttc acatcttttt acgtcgtact
       61 cacctatctt aattcacaat aaaaaataac catattggag ggcatcatgc ctgacgctaa
      121 aaaacagggg cggtcaaaca aggcaatgac ttttttcgtc tgcttccttg ccgctctggc
      181 gggattactc tttggcctgg atatcggtgt aattgctggc gcactgccgt ttattgcaga
      241 tgaattccag attacttcgc acacgcaaga atgggtcgta agctccatga tgttcggtgc
      301 ggcagtcggt gcggtgggca gcggctggct ctcctttaaa ctcgggcgca aaaagagcct
      361 gatgatcggc gcaattttgt ttgttgccgg ttcgctgttc tctgcggctg cgccaaacgt
      421 tgaagtactg attctttccc gcgttctgct ggggctggcg gtgggtgtgg cctcttatac
      481 cgcaccactg tacctgtcag aaattgcgcc ggaaaaaatt cgcggcagta tgatctcgat
      541 gtatcagttg atgatcacta tcgggatcct cggtgcttat ctttctgata ccgccttcag
      601 ctacaccggt gcatggcgct ggatgctggg tgtgattatc atcccggcaa ttttgctgct
      661 gattggtgtc ttcttcctgc cagacagccc acgttggttt gccgccaagc gccgttttgt
      721 tgatgccgaa cgcgtgctgc tacgcctgcg tgacaccagc gcggaagcga aacgcgaact
      781 ggatgaaatc cgtgaaagtt tgcaggttaa acagagtggc tgggcgctgt ttaaagagaa
      841 cagcaacttc cgccgcgcgg tgttccttgg cgtactgttg caggtaatgc agcaattcac
      901 cgggatgaac gtcatcatgt attacgcgcc gaaaatcttc gaactggcgg gttataccaa
      961 cactaccgag caaatgtggg ggaccgtgat tgtcggcctg accaacgtac ttgccacctt
     1021 tatcgcaatc ggccttgttg accgctgggg acgtaaacca acgctaacgc tgggcttcct
     1081 ggtgatggct gctggcatgg gcgtactcgg tacaatgatg catatcggta ttcactctcc
     1141 gtcagcgcag tatttcgcca tcgccatgct gctgatgttt attgtcggtt ttgccatgag
     1201 tgccggtccg ctgatttggg tactgtgctc cgaaattcag ccgctgaaag gccgcgattt
     1261 tggcatcacc tgctccaccg ccaccaactg gattgccaac atgatcgttg gcgcaacgtt
     1321 cctgaccatg ctcaacacgc tgggtaacgc caacaccttc tgggtgtatg cggctctgaa
     1381 cgtactgttt atcctgctga cattgtggct ggtaccggaa accaaacacg tttcgctgga
     1441 acatattgaa cgtaatctga tgaaaggtcg taaactgcgc gaaatcggcg ctcacgatta
     1501 atctcctcag gcttcctccc atcgcggagg aagccacctc ttgcagtcat cttttcttcg
     1561 ctctatcctc tgccgctatg aaaacatccc gtctccctat cgccatccag caggccgtta
     1621 tgcgccgcct gcgggaaaaa ctcgcccagg ccaacctgaa gctagggcgt aactacccgg
     1681 agccaaaact ctcttacacc cagcgcggaa cctccgccgg aacagcctgg ctggaaagct
     1741 atgaaatccg cctcaatccc gttttgctgt tggaaaacag tgaagctttt attgaagaag
     1801 tggtaccgca cgaactggca catttgctgg tatggaaaca tttcggccgc gtagcgccac
     1861 atggcaaaga gtggaagtgg atgatggaaa gcgtgctggg cgttcccgcc cgtcgtacgc
     1921 atcagttcga actgcaatcc gtgcgtcgca acaccttccc ctaccgctgc aagtgccagg
     1981 agcatcagct taccgtacgc cgccataatc gcgtagttcg tggcgaggcc gtctatcgct
     2041 gtgttcactg cggtgaacag ctggttgcga aataaccatc tgaactatca ggaactttcc
     2101 tgatctggct gattgcatac caaaacaact ttcgctacgt tgctggctcg ttttaacacg
     2161 gagtaagtga tgtaccgtta tttgtctatt gctgcggtgg tactgagcgc agcattttcc
     2221 ggcccggcgt tggccgaagg tatcaatagt ttttctcagg cgaaagccgc ggcggtaaaa
     2281 gtccacgctg acgcgcccgg tacgttttat tgcggatgta aaattaactg gcagggcaaa
     2341 aaaggcgttg ttgatctgca atcgtgcggc tatcaggtgc gcaaaaatga aaaccgagcc
     2401 agccgcgtag agtgggaaca cgtcgttccc gcctggcagt tcggtcacca gcgccagtgc
     2461 tggcaggacg gtggacgtaa aaactgcgct aaagatccgg tctatcgcaa gatggaaagc
     2521 gatatgcata acctgcaacc gtcagtcggc gaggtgaatg gcgatcgcgg caactttatg
     2581 tacagccagt ggaatggcgg cgaaggccag tacggtcaat gcgccatgaa ggtcgatttc
     2641 aaagaaaaag ctgccgaacc accagcgcgt gcacgcggtg ccattgcacg cacctacttc
     2701 tatatgcgcg accaatacaa cctgacactc tctcgccagc aaacgcagct gttcaacgca
     2761 tggaacaaga tgtatccggt taccgactgg gagtgcgagc gcgatgaacg catcgcgaag
     2821 gtgcaaggca atcataatcc gtatgtgcaa cgcgcttgcc aggcgcgaaa gagctaacct
     2881 acactagcgg gactcttttt gttaacccct accccacgcg tacaaccgcg tggggagacg
     2941 acgcggattt ttaactatgc gtatcccccg catttatcat cctgaaccac tgaccagcca
     3001 ttctcacatc gcgctttgcg aagatgccgc caaccatatc gggcgcgtac tgcgcatggg
     3061 gccggggcag gcgttgcaat tgtttgacgg tagcaaccag gtctttgacg ccgaaattac
     3121 cagcgccagc aaaaaaagcg tggaagtgaa ggtgctggaa ggcaagatcg acgatcgcga
     3181 atctccgctg catattcacc tcggtcaggt gatgtcgcgt ggtgaaaaaa tggaatttac
     3241 gatccagaaa tcgatcgaac tcggtgtaag cctcattacg ccactttttt ctgagcgctg
     3301 cggcgttaaa ctggatagtg aacgtctgaa caagaagctc cagcagtggc agaagattgc
     3361 aattgctgcc tgtgagcagt gtggtcgtaa ccgggtgccg gaaatccgcc cagcgatgga
     3421 tctggaagcc tggtgtgcag agcaggatga aggactgaaa ctgaatcttc acccgcgcgc
     3481 cagtaacagc atcaatacgt tgccgttacc ggttgaacgc gtccgcctgc tgattggccc
     3541 ggaaggcggt ttatcggcag atgaaattgc catgactgcc cgctatcaat ttactgatat
     3601 cctgttggga cctcgcgttt tgcgtacaga gacaactgcg ctcaccgcca ttaccgcgct
     3661 acaagtacga tttggcgatt tgggctaacg gagaagaata atgatcaagc tcggcatcgt
     3721 gatggacccc atcgcaaaca tcaacatcaa gaaagattcc agtttcgcta tgttgctgga
     3781 agcacagcgt cgtggttacg aacttcacta tatggagatg ggcgatctgt atctgatcaa
     3841 tggcgaagcc cgcgcccata cccgcacgct gaacgtgaag cagaactacg aagagtggtt
     3901 ttcgttcgtc ggtgaacagg atctgccgct ggccgatctc gatgtgatcc tgatgcgtaa
     3961 agacccgccg tttgataccg agtttatcta cgcgacctat attctggaac gtgccgaaga
     4021 gaaagggacg ctgatcgtta acaagccgca gagcctgcgc gactgtaacg agaaactgtt
     4081 taccgcctgg ttctctgact taacgccaga aacgctggtt acgcgcaata aagcgcagct
     4141 aaaagcgttc tgggagaaac acagcgacat cattcttaag ccgctggacg gtatgggcgg
     4201 cgcgtcgatt ttccgcgtga aagaaggcga tccaaacctc ggcgtgattg ccgaaaccct
     4261 gactgagcat ggcactcgct actgcatggc gcaaaattac ctgccagcca ttaaagatgg
     4321 cgacaaacgc gtgctggtgg tggatggcga gccggtaccg tactgcctgg cgcgtattcc
     4381 gcaggggggc gaaacccgtg gcaatctggc tgccggtggt cgcggtgaac ctcgtccgct
     4441 gacggaaagt gactggaaaa tcgcccgtca gatcgggccg acgttgaaag aaaaagggct
     4501 gatttttgtt ggtctggata tcatcggcga ccgtctgact gaaattaacg tcaccagccc
     4561 aacctgtatt cgtgagattg aagcagagtt tccggtgtcg atcaccggaa tgttaatgga
     4621 tgccatcgaa gcacgtttac agcagcagta acccacctta gcgagaagga tctcgttgag
     4681 actctgagtg acagcgccct tctttccacg catactgggc gctgttgctt ttttgaacca
     4741 ggaaacagaa cctctgacaa tgaatttaca gcatcacttt cttattgcca tgcctgctct
     4801 ccaggatccg attttccgtc gttccgtggt ctacatttgc gaacataata ccaatggtgc
     4861 aatggggatc atcgtcaaca agccgctgga aaatctcaaa attgaaggga ttctggaaaa
     4921 gctgaagatc acgccggagc cgcgtgatga atcaatccgt ctggataaac cggttatgct
     4981 cggcggtccg ctggctgaag atcgcgggtt tattttgcat actccgccct ccaattttgc
     5041 ttccagcatt cgcatttcag acaacacagt gatgaccact tcccgcgatg tgctggaaac
     5101 gctcggcacc gataaacaac cgtctgacgt attggtggct ctgggttatg cctcctggga
     5161 aaaaggtcaa ctggaacaag aaattctcga taacgcgtgg ctaacggccc cggcggatct
     5221 gaatattctg ttcaaaacgc cgattgccga ccgctggcgc gaggcggcaa aactgattgg
     5281 tgtggatatt ctcaccatgc ctggtgtggc agggcacgcc tgatgagtgg aaccttactc
     5341 gccttcgact tcggcaccaa aagcattggc gtagcggtcg gccaacgcat taccggcact
     5401 gctcgccctt tgcctgcaat taaagcacag gacggtacgc cggactggaa cctcatcgaa
     5461 cgtttactga aagagtggca gccggacgaa atcatcgtcg gtttgccgct gaatatggac
     5521 ggcaccgagc aaccattgac tgccagagcg cgtaaatttg ccaaccgtat tcatggtcgt
     5581 ttcggtgttg aagtaaagct ccatgacgaa cgtcttagca ccgtggaagc ccgttccggt
     5641 ctgtttgaac agggcggcta tcgggcgctc aacaaaggca aagtcgactc tgcctctgcg
     5701 gttattattc tcgaaagcta tttcgagcag ggatattaag gcgatctaaa agcctggcgg
     5761 atagtcgtta ccgccatctt gccggatgcg gcgtgaacgc cttatccagc acacatctgg
     5821 cagcggctgt aggtctgata agacgcgtta gcgtcgcatc agacatttat tgcctttata
     5881 ggccctataa acttgcgcat cgggcatggc aacgtcacaa acgcccttcc cccacccgct
     5941 gctgataact ctgctgaaac gttaacatcc ccacctgctg cccggtttga ataacatgcg
     6001 gtaactggtg ggttttccct tcgcgaatca aattccccac cgcgggagtg ttaatcaata
     6061 attcaaacag cgccacgcgt ccttcctgtt tatccacttc cagcttttgt gacaacacgg
     6121 cccgtaaact acctgccagt tgattacgta cgggatcttt ttcctgcgcc ggaaatgaat
     6181 ccaccagtcg ctcaactgcc tgcgcggcac cacgcgtatg taatgttgcc agcaccaaat
     6241 gcccggtttc tgccgccgtc agtgccagac ggattgtctc gctgtcacgc agctctccga
     6301 gcaaaatcac atcaggatct tcccgcaatg cggcccgcaa tcccgatgcg aacgtcatac
     6361 agtgcaaacc aatttcccgc tgctggatca aacatcgctg gctggcatag atatattcaa
     6421 caggatcttc cagcgtcaga atatgcgcat cggcatgttg atagagatag ccaaccatcg
     6481 ccgctagcgt ggtagatttg ccgctccccg tcgcccccgt caccagaatc aggccattct
     6541 cgctcttgag taattccggc aataccgttg gtgcgccaag ctgttcgagc tgtggacagt
     6601 gcgaaggtaa caaccgtaat gccagagaaa taccttgccg ttgcgcgaac gcactgccac
     6661 gcaaccgctg gttttccgcc agcgacacgg caaaatccag ctggccattt tccagcaata
     6721 ttgcccgctg atcgtcatcc agccactccc gcagtagctc ttcgacgtcc ggcgcatcaa
     6781 acggcgcagc ttccattagc ccgcgaatgc gccatcgtgc ggaccaggcg ctgcacaggt
     6841 gtagatccga gacgttatgc tttacactaa gggccacaat ttcttccata ttcatactaa
     6901 gatcctcgga aaatgaacga tattgcgcat aacctggcac aggtccggga caaaatctca
     6961 gcggctgcaa cgcgttgcgg ccgttctcca gaagaaatta cgctgcttgc agtcagtaaa
     7021 acaaaacctg cgagcgccat cgcagaagcc attgatgccg ggcagcgtca atttggtgaa
     7081 aactacgttc aggaaggggt agataaaatt cgccactttc aggaactggg cgtaacagga
     7141 ttagaatggc attttattgg cccgttgcag tctaataaaa gccgcctggt ggcagagcat
     7201 ttcgactggt gtcataccat cgaccgtttg cgcatcgcta cccgcctcaa cgatcagcgc
     7261 ccggcagaac ttccccctct taacgtactg attcaaatta atattagtga tgaaaacagt
     7321 aagtccggga ttcaactggc agaactggac gagctggcag ctgcggtcgc tgaactaccg
     7381 cgtttacgtc tgcgcgggct gatggcaatc cctgcacctg agtcagaata tgtaaggcag
     7441 tttgaagttg cacgccaaat ggctgtagca tttgccggac tgaaaacgcg ctacccgcat
     7501 atcgacacgc tctctctggg aatgtcggac gatatggaag ccgccattgc ggcaggtagc
     7561 acgatggttc gtatcggcac tgcaattttt ggtgcgcgtg attactctaa aaaataagga
     7621 attaaaggaa cgccatgaat acgttgactt tcctgctttc aacggtcatt gagctgtata
     7681 ccatggtgct gttattacgc atctggatgc agtgggctca ttgtgatttt tacaacccct
     7741 tctcacagtt tgtagtgaag gtaacgcagc caattatcgg gccactgcgc cgcgttattc
     7801 cggcaatggg ccccattgac agcgcctcgc tgctggttgc ctatattctc agttttatca
     7861 aagccatcgt gctgtttaaa gtggtgacct tcctgccaat catctggatt gccggtttac
     7921 tgattctgct gaaaaccatc ggcctgctga ttttctgggt cctgctggtg atggcgatta
     7981 tgagctgggt aagccagggt cgtagcccga ttgaatacgt gctgattcag ctggccgatc
     8041 cgctgctgcg cccgattcgc cgcctgctac cggcaatggg tgggattgat ttctcgccga
     8101 tgatcctcgt tctgctgctg tatgtcatca atatgggtgt cgcagaagta ttacaggcaa
     8161 ccggaaacat gctgctgccg gggctgtgga tggcgttatg agtgccgtaa cagttaatga
     8221 tgacggtctg gttttacggc tctatattca gccgaaagcc agccgtgatt ctattgtcgg
     8281 tttacatggc gacgaagtta aagtcgccat taccgcgccg ccggttgacg gtcaggccaa
     8341 cagtcatctg gtgaagtttc tcggtaagca attccgggtt gccaaaagcc aggtggtgat
     8401 tgaaaaaggc gaacttggcc gccacaaaca aattaaaatc attaatccgc aacaaatccc
     8461 gccagaaatc gcggcgttaa ttaattaggt atcctatgca aaaagttgtc ctcgcaaccg
     8521 gcaatgccgg taaagtgcgt gagctggcgt cgctgcttag cgacttcggt cttgatatcg
     8581 tggcccaaac agacctcggc gtagattccg ctgaagaaac cggcctgacc tttatcgaaa
     8641 acgcgattct gaaagcgcgc catgcggcaa aagtgaccgg tttaccggca attgccgacg
     8701 actctggtct ggcggtagat gtgcttggcg gcgcgccggg catttactcc gcgcgttatt
     8761 ccggtgaaga cgcgaccgat ctaaagaatc tgcaaaaact gctggaaaca ctgaaagacg
     8821 taccggatga ccaacgtcag gcgcgtttcc actgcgtgct ggtatatctg cgtcacgcgg
     8881 aagatccgac tccgctggta tgccacggta gctggccggg cgtgattact cgtgaaccag
     8941 cgggcactgg tggctttggt tatgatccaa tcttcttcgt accttccgaa gggaaaaccg
     9001 ccgccgaact gacccacgaa gaaaagagcg ccatttccca ccgtggtcag gcattgaaac
     9061 tgctgctgga cgctttacgt aatggttaaa ttaccgccgc tgagtctcta cattcacatc
     9121 ccgtggtgcg tgcagaaatg cccgtactgc gatttcaact ctcacgcgtt gaaaggagaa
     9181 gtgccgcacg acgattacgt tcagcatctg ctttgcgatc tggacaacga tgtggcttac
     9241 gctcagggcc gtgaagtaaa gacaatcttt attggcggtg gtacgccgag cctgctttcc
     9301 ggcccggcga tgcaaacgct gctggacggc gtgcgtgcgc gtttgccgct ggcagcggat
     9361 gcagaaatta ctatggaagc gaaccctggt acggtagaag ccgatcgctt tgtcgattat
     9421 cagcgtgctg gtgtgaaccg catctctatt ggtgtgcaga gttttagcga agacaagctg
     9481 aaacgacttg ggcgtattca tggcccgcaa gaagcgaaac gcgcggcaaa gctggcgagc
     9541 gggctggggc tgcgtagctt taacctcgat ttgatgcatg gactaccgga tcaatcactg
     9601 gaagaggcgc ttggcgatct acgccaggcc attgaactga atccgccgca tctttcctgg
     9661 tatcaactga ccatcgaacc taatacgctg tttggttcac gcccgccggt actgccggac
     9721 gacgacgcgc tatgggatat attcgaacag gggcatcagt tattaaccgc agcgggttat
     9781 cagcaatatg aaacttccgc ttacgccaaa tccagttatc agtgccagca caatctcaac
     9841 tactggcgct ttggcgacta catcggtatt ggctgcggcg cgcacggcaa agtgaccttc
     9901 ccggatgggc gcattctgcg taccactaaa acgcgtcatc cgcgtggttt tatgcaagga
     9961 aggtatctgg aaagccagcg tgatgtcgaa gccgcagata agccgtttga gttctttatg
    10021 aatcgcttcc gtctgctgga ggccgcgccg cgcgtggagt ttattgcgta taccgggctt
    10081 tgcgaagatg tgattcgccc acagttagac gaggcgattg cccagggtta tctcaccgaa
    10141 tgtgcggatt actggcagat aacggaacat ggtaaattat tcttaaactc acttcttgaa
    10201 ttattcctcg ctgagtaatc attaatgcgt ctgcgggcgc attaatcact tcttcccccc
    10261 ccccccg
//
LOCUS       DAZGJW010000161        10245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZGJW010000161 DAZGJW010000000
VERSION     DAZGJW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.161"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1171
                     /gene="ybaT"
                     /locus_tag="ACHKIK_004207"
     CDS             <1..1171
                     /gene="ybaT"
                     /locus_tag="ACHKIK_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO9085268.1"
                     /translation="MEASTWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGL
                     GNGVFSLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMT
                     LFNSLSNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGI
                     TFLAYAGFGMMANAADKVKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEK
                     YADTAVAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLM
                     NKSLWRQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIH
                     ASLPILIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            1202..1299
                     /locus_tag="ACHKIK_004208"
                     /pseudo
     CDS             1202..1299
                     /locus_tag="ACHKIK_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1296..1703
                     /gene="cueR"
                     /locus_tag="ACHKIK_004209"
     CDS             1296..1703
                     /gene="cueR"
                     /locus_tag="ACHKIK_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO9085269.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(1704..2162)
                     /gene="ybbJ"
                     /locus_tag="ACHKIK_004210"
     CDS             complement(1704..2162)
                     /gene="ybbJ"
                     /locus_tag="ACHKIK_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO9085270.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(2159..3076)
                     /gene="qmcA"
                     /locus_tag="ACHKIK_004211"
     CDS             complement(2159..3076)
                     /gene="qmcA"
                     /locus_tag="ACHKIK_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO9085271.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            3222..3899
                     /gene="fetA"
                     /locus_tag="ACHKIK_004212"
     CDS             3222..3899
                     /gene="fetA"
                     /locus_tag="ACHKIK_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO9085272.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTNGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            3886..4665
                     /gene="fetB"
                     /locus_tag="ACHKIK_004213"
     CDS             3886..4665
                     /gene="fetB"
                     /locus_tag="ACHKIK_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO9085273.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(4728..5582)
                     /gene="cnoX"
                     /locus_tag="ACHKIK_004214"
     CDS             complement(4728..5582)
                     /gene="cnoX"
                     /locus_tag="ACHKIK_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO9085274.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAETVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(5643..6452)
                     /gene="ybbO"
                     /locus_tag="ACHKIK_004215"
     CDS             complement(5643..6452)
                     /gene="ybbO"
                     /locus_tag="ACHKIK_004215"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO9085275.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(6442..7065)
                     /gene="tesA"
                     /locus_tag="ACHKIK_004216"
     CDS             complement(6442..7065)
                     /gene="tesA"
                     /locus_tag="ACHKIK_004216"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO9085276.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            7036..7722
                     /gene="ybbA"
                     /locus_tag="ACHKIK_004217"
     CDS             7036..7722
                     /gene="ybbA"
                     /locus_tag="ACHKIK_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO9085277.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            7719..10133
                     /gene="ybbP"
                     /locus_tag="ACHKIK_004218"
     CDS             7719..10133
                     /gene="ybbP"
                     /locus_tag="ACHKIK_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO9085278.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVEPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2247 a         2636 c         2857 g         2505 t
ORIGIN      
        1 aatggaagcc tcgacctggg tcgcctttgc ttttggcggt attgtggcga tgttttccgg
       61 ttatgcctat gcgcgtctgg gggcgagcta tcccagcaat ggcggcatta tcgacttctt
      121 tcgtcgcgga ttaggcaacg gcgtcttttc gctggcgctc tcgttactgt acctgttgac
      181 gctggcggtg agcatcgcca tggtcgcccg tgcttttggc gcttatgccg tgcagttttt
      241 gcatgaaggc agccaggagg agcaccttat tttgctctac gcgttgggga tcattgcggt
      301 gatgacgctt ttcaactcct taagcaacca tgcggtaggg cggctggaag tgatcctcgt
      361 cggcattaaa atgatgatcc tgttattgct gattattgcc ggtgtctggt cgctgcaacc
      421 ggcgcatatt tccgtctctg cgccccccag ctccggtgcg ttcttctcct gtatcgggat
      481 aaccttcctt gcctatgcgg gctttggcat gatggcgaac gcggcggata aagtgaaaga
      541 tccgcaggtc attatgccgc gggcgtttct ggtggcgatt ggcgttacca cgttgcttta
      601 tatctcgctg gcactggttt tgcttagcga tgtatcggca ttagagttag aaaaatatgc
      661 cgataccgcc gtagcgcagg ctgcttctcc gctgctcgga catgtgggtt atgtgatcgt
      721 cgtcatcggc gctttactgg cgacggcttc agccattaac gcgaacctgt tcgccgtgtt
      781 taacatcatg gacaacatgg gcagcgaacg cgaactgccg aagctaatga ataaatccct
      841 gtggcggcag agtacctggg gcaacatcat tgtcgtggtg ttgattatgc tgatgacggc
      901 ggcactaaat ttaggctcac tcgccagcgt tgccagcgcc acctttttga tttgctatct
      961 ggcggtgttt gtggtggcga tccgcctgcg tcatgatatt cacgcctcgt tgccgattct
     1021 tatcgttggt acgttggtga tgttgttggt gatcgttggc tttatctaca gtctgtggtc
     1081 ccagggtagc cgtgcgttga tatggattat tggctcactc ttactcagcc ttattgtggc
     1141 aatggtcatg aagcgcaata aaaccgtata acatctctct gtgcgcagta cttcctgtat
     1201 tattgtggtg gcggtcgata ttcgcactgg caaaaaaacg tgcttgaata tctgttgaaa
     1261 ccctttaaca aagcacagga ggcgttgcgc gaacgatgaa catcagcgat gtagcaaaaa
     1321 ttaccggcct gaccagcaaa gccattcgct tctatgaaga gaaggggctg gtgacgccgc
     1381 cgatgcgcag cgaaaacggt tatcgcacct acacgcagca gcatctcaac gaactgacct
     1441 tactgcgcca ggcacggcag gtgggcttta acctggaaga gagcggcgag ctggtgaatc
     1501 tgtttaacga cccgcagcgg cacagcgccg acgtcaaacg gcgcacgctg gagaaggtgg
     1561 cggagatcga acgacacatt gaggagctgc aatccatgcg cgaccagctg ctggcactgg
     1621 cgaatgcctg ccctggcgat gacagcgccg actgcccgat tatcgaaaat ctctccggct
     1681 gctgtcatca tcgggcaggg tgattaagac gagacggctc ggatatgtag ggttatccct
     1741 tctatcgcaa tgacttcaac atgcgtacct gcaccgagat cctcgctggc gctgacaggc
     1801 catgaactgt caccgacgcg catatgaccg cgcccgttga ccagcggaga ttccagcaca
     1861 aaacgtcggc caatcagctg ctgcccgcgc tggtttaaat gactatcgct gtgcttttgt
     1921 tcgcgcaccc gccgcgacaa ccatttccac cacagccagg cggcgagcag cgtcaggatg
     1981 gcaaacatta ccccttgcca ctcccaaccc agcggcacca gccagaccac caggccggta
     2041 atcaccgctg ccacgccgct ccacaacaaa taaccatttc cgcccagcat ctcggctgcc
     2101 agcagcaaac cgccgagact gagccagaaa atatgtggat gaacgaccat taactccatc
     2161 atggctgagt ccgtttgttg gcgctgtctt tcaccagctc ggcaatcccg gcaatcgacc
     2221 ccatcaggct gctggcctct aatggcatca tcactacttt gctgttactg gaggaaccga
     2281 tctgctgtaa cgcttcggtg tatttctgcg ctacgaagta gttcaccgcc tgaatatcac
     2341 cggaggcgat agcttcagac accattttgg tggcgcgggc ttctgcttcg gcggaacgtt
     2401 cacgcgcttc agcctgtaaa aacgccgact gacgttcgcc ttccgctttc aggatttgcg
     2461 actgtttttc accttcggct ttgaggattt ccgcctgacg gatcccttcc gcttcaagaa
     2521 tgtaagcgcg tttggtacgt tccgctttca tctgcgcgtt cattgaagag ataagctctg
     2581 ccggtgggcg cacgtcgcga atttcaatac gggtgacttt aatcccccac gggttggtgg
     2641 cttcatcgac aatacgcagc aggcgtgagt tgatgctgtc gcgctgagag agcatttcgt
     2701 caagttccat tgaacccaac acggtacgga tgttagtcat ggtcaggttg atgatcgcca
     2761 gctccagatt gctgacttca taagccgcgc gcggcgcgtc aatcacctga ataaaacaga
     2821 ctgcgtcgat ggtaacgttg gcgttatctt tcgagataac ttcctgggaa gggatatcga
     2881 gcacttgctc catcatattg atcttgcgac caatgcgatc cataaacggc accaccagac
     2941 tgagccccgg ctgtaacgtt ttggtatagc gaccaaaacg ttctaccgtc cattgatagc
     3001 cctgcggcac gattttgacg cccgcgccga caatgaccag cgcgacaaaa atgagaatcg
     3061 ggataaagat aagcatcgga aaaacctcct gttgtaccgt ccataatcag caaaattgct
     3121 gcttgattaa acaaattata cctgattact gaaagagagt tcccccttat tcctgcgaag
     3181 gataaactgt ttttagtaaa aatcagaaaa agggaacagt gatgcaggaa aatagtcctt
     3241 tacttcagct acaaaacgta ggatatctgg cgggtgatgc gaagattctt aataacatca
     3301 atttttcgct gcgtgctggc gaatttaagt taattaccgg tccttccggt tgtggcaaaa
     3361 gtacgctgct aaaaatagtt gcttcattga tcagcccaac caacggaacg ttactgtttg
     3421 aaggtgagga tgtcagcaca ctaaagccag aaatctaccg ccagcaagtc tcttactgcg
     3481 cccagacgcc gacgctgttt ggcgatacgg tatacgataa tctgatcttt ccctggcaga
     3541 tccgtaacca gcagcctgac ccggccattt ttctcgattt tctcgaacgc ttcgctttgc
     3601 cggacagcat tttgacgaaa aatatcgccg agctatctgg tggtgaaaaa caacgcatct
     3661 cactgattcg taacctgcaa tttatgccga aggttttatt gctggatgaa atcaccagtg
     3721 cgctggatga aagtaataaa cataacgtta atgagatgat ccatcgttat gtgcgcgagc
     3781 aaaatattgc cgtgctgtgg gtgacacacg ataaagacga aattaatcat gcggataaag
     3841 tgattacact gcaaccgcat gccggagaaa tgcaggaagc acgctatgaa ctcgcataat
     3901 attactaacg aatcattggc actggcatta atgctggtgg tggtggcaat cttaattagc
     3961 cataaagaaa aactggcgct ggagaaagat attttctgga gcgtcgggcg agcgataatt
     4021 cagctgatta ttgtcggcta tgtgctgaag tatattttca gcgtggatga tgccagcctg
     4081 acattattga tggtgttatt tatctgcttt aatgcggcgt ggaacgcgca aaaacgcagt
     4141 aaatatattg ctaaagcttt tatctcatcg tttattgcca ttacggtcgg ggcgggaatt
     4201 accctggcgg tgctgattct ctctgggtcg attgaattta tcccgatgca ggtgatccct
     4261 atcgccggga tgattgccgg taacgccatg gtagcggtgg ggttgtgtta caacaattta
     4321 gggcaacggg tcattagcga acagcaacag attcaggaga aactgagtct tggtgcgacg
     4381 ccgaagcagg cttcagcgat attgattcgc gacagtattc gcgcggcttt aattccgacg
     4441 gtcgattcag caaaaacggt tggcttagtg agtttacctg gaatgatgtc cgggctgata
     4501 tttgccggga ttgatccggt gaaggcgatt aaatatcaga ttatggtgac ctttatgctg
     4561 ctctcaaccg ccagcttgtc gaccattatt gcctgctatt taacctatcg taagttttat
     4621 aattcgcgcc accagttggt ggtgacgcaa ttgaagaaga aatgatgcga tgccggatgc
     4681 gcagcatcgc atccggcgtt gtggttcatg tgccgatcaa cggaatatca atacaacaat
     4741 gcatacagct ggcggcgata cttcgacgcc agtgcatcac ccgtacccag cgcagcgagg
     4801 atctcctgaa acgttttacg cgtctgaccg tctgcggcgg tgagatcttt acgcagatgc
     4861 ccgaacagca actccagcgc ctcttcattg cgcccaacct ggtgcagttg cagagccagt
     4921 tgcgtcgcca gtgcggcatc ttctggattc tccgccacct gctgttgcaa ctgttgaatt
     4981 tccggcgtat cagccgcctg cttcagcagt tcgatttgcg ccaccagccc ctggtagcgg
     5041 gtgtcctgat cctgcaacgg aatggttttc agcaccgttt ccgcatcttc agaacggttc
     5101 agcgcaatca gcgtttctgc cagcagcagg ccgatctccc cgttctgatt cgacaactgc
     5161 caggcgtctt tcagcaatgg cagggcatcg gtgtaattgc cttcctgcat cagttgcatc
     5221 gcctgctgcg ctttcagctc ttcttcgcgc ggcagcactt tatccagcag ggcgcggatc
     5281 gcctcttccg gttgcggccc ctggaagcca tctaccggtt gcccgttctg gaacagatac
     5341 acggtcggaa tcgcgcgcag accaaactgg gcggcaatca tctgctccgc gtcgcagtcc
     5401 agcttcgcca gaataaattg cccgttgtac tgcgccgcga ggctttccag gatcggggtt
     5461 aactgcaaac agtgctggct acgttcagac caaaaataga acagcaccgg agtggtcatc
     5521 gactgttcaa gaacctgttg caggttagat tcgttaatgt tgacaatatt ttctacggac
     5581 atggagtcgc tctctgttgt cgatattttc tttgacatgg gggcttaagc gcgcgcttca
     5641 actcacccct gcaatatttt gtccatcacg cgccccggca gcaggcgctt gagcaccatt
     5701 actgcccagg tcaccagcgt taccggatag cgcatcttcg gcttctcgct aataaaagca
     5761 tggcgtactt tgtccaccac cgcttccggt cccaacgtaa agcgggcggc gatgccggga
     5821 ttttcgactg gtttatcact ttgcgtctgg ttgacgttgt cggtgaagcg agtacgaatg
     5881 ggaccgggtt cgatcaggct gactttaatt ccgctgtggc gcagctccat gcgcagtgcg
     5941 tctgaccacg cctccagcgc atatttactg gccgcgtaag cgccacgacc cggcgtggag
     6001 attaatccca tcaccgatga tgtcatcaca atacgccctt caccgtgcgg taacatcgcg
     6061 ggtaacaggc gcatggtgag ctggtgtgcg ccgaaaaagt tggcggaaaa ctgctgttcc
     6121 atctgcgcac ggctgatggt ggaaaggggg ccatacatgc cgaatccggc attgttaaag
     6181 atcccataca gacaattatc ggtcagggcg atcacctcgt ctgctgcgcg atcaacactt
     6241 tctggtgaat ccagatcgat caacacgccg gtaaatccca tgttgttcat acgctcaaca
     6301 tcatccggtt ttcggcaacc tgccagcaca tgaaaaccct ggcgttttaa ttcgagcgcg
     6361 ctttccaggc caattccact ggaacatccg gtaattaaga ccgatttttg cataacttta
     6421 cctgtcagga tctccgttgc tttatgagtc atgatttact aaaggctgca actgcttcgc
     6481 catccagtcg gcaataaacg gctgggcgtc gcggttggga tgaataccgt catcctgcat
     6541 ccattgtggc ttgaggtaga cctcttccat aaaaaagggc agcagcggaa catcaaactc
     6601 tttggcgagt ttggggtaaa tggcgctaaa ggcttcatta taacggcgac catagtttgc
     6661 aggcagacgt atttgcatta acaatggttc agcgttggcg gctttgacat cctgcaaaat
     6721 ctggcgcagc gtttgctcgg tttgctgtgg ctgaaaacca cgcaaaccgt cattgccgcc
     6781 cagttcaacc agcacccagc gcggctgatg ctgtttcagc agagccggaa ggcgcgccag
     6841 tccttgttgc gaggtgtcgc cgctgatgct ggcattaact accgacgttt tactctgcca
     6901 cttatcattc aacaaggcag gccaggccgc gctggcagac attcgatacc cggcgctcag
     6961 gctatcaccc agaatcaata acgtgtccgc tgcggcggca cggaaggtta acaggaccag
     7021 gaacaggaag ggcaaatgcc agcggaaaac attgttgaag ttcatcatct taagaagtcc
     7081 gtcggtcagg gggagcatga actctccatc ctcaccggag ttgagctggt tgtcaaacgt
     7141 ggcgagacca tcgcactggt gggcgaatcg ggatcgggta agtcaacctt gctggcgatc
     7201 ctcgccgggc ttgatgacgg cagcagtggc gaagtgagtc tggtgggaca accgctacat
     7261 aatatggacg aagaagcgcg ggcaaagttg cgcgcgaagc acgtcggctt tgtttttcag
     7321 tcatttatgt taattcctac ccttaacgcg ctggaaaatg tcgagcttcc ggctctgctg
     7381 cgcggtgaga gtagcgcgga aagtcgtaac ggggcgaaag cgttgctcga acagttaggg
     7441 ctgggtaaac gtctggatca tcttccggca cagctttccg gcggtgaaca gcaacgagtg
     7501 gcgctggcac gagcctttaa tggtcgacct gatgtgctgt ttgccgacga acccaccggc
     7561 aaccttgacc gccagacggg cgataaaatt gccgacctgc tgttttccct caaccgtgaa
     7621 catggcacca cgttgattat ggtgactcac gacctgcaac tggcggcacg ctgcgaccgc
     7681 tgcttacggc tggtgaacgg gcagttgcag gaggaagcat gattgcacgt tggttctggc
     7741 gcgaatggcg ctcgccgtcg ctattaattg tctggctggc gctaagcctg gcggtggcct
     7801 gcgtgctggc gctgggcaat atcagcgatc gcatggagaa gggcttaagc cagcaaagcc
     7861 gtgagtttat ggcgggcgat cgggcgttgc gcagttcgcg cgaagtgccg caagcatggc
     7921 tggaggaagc gcaaaagcgc ggcctgaaag tcggcaagca gctgactttc gccaccatga
     7981 catttgcagg cgacacaccg cagctggcga acgtcaaagc ggtggatgat atctacccga
     8041 tgtatggcga tctgcaaact aatccccctg gcctgaaacc gcaggcgggc agcgtattgc
     8101 tggccccacg cctgatggca ctgcttaacc tgaaaacggg cgacaccatt gacgtgggcg
     8161 atgccacctt gcggattgcc ggagaagtga ttcaggaacc ggattccggt tttaacccct
     8221 tccagatggc tccgcgtctg atgatgaatc tggcggatgt cgataaaacc ggagccgtgc
     8281 agccggggag tcgggtcacc tggcgttata aattcggcgg caacgagaac cagctcgacg
     8341 gctatgagaa atggttgtta cctcagctta aacccgaaca acgctggtac ggtctggaac
     8401 aggacgaagg cgcgctgggg cgatcgatgg aacgctcgca acagttcctg ctgctttcgg
     8461 cgcttctgac cttgctgctg gcagtggcag cggtggcggt agcgatgaat cattactgtc
     8521 gcagtcgcta cgatctggtg gcgatcctca aaacgctggg ggcagggcga gcgcaactgc
     8581 gtaagctaat cgtcggtcag tggttgatgg tgctggcgct ttcagccgtt accggtgggg
     8641 cgataggcct gttgttcgaa aacgtgttga tggtgctgct caagcccgtt ctgcctgctg
     8701 cactaccgcc agccagcctc tggccgtggc tgtgggcgct gggtaccatg acggtcatct
     8761 cgctgctggt ggggctacga ccgtaccgcc tgttgctggc aacgcagcct ttacgcgtat
     8821 tacgtaatga tgtggtagcg aacgtctggc cgctgaagtt ttatctgccg attgtcagtg
     8881 tggtggttgt gctgctgctc gccggattaa tgggtggcag catgctgctt tgggcggtgc
     8941 tggcgggcgc ggtagtactg gctttgctgt gcggtgtgct gggctggatg ctgctgaatg
     9001 tacttcgccg catgacgctg aaatcgctac ctctgcgcct ggcggttagc cgcctgttac
     9061 gtcagccgtg gtcaacatta agccagcttt cggcattttc gctctccttt atgctgctgg
     9121 cgctgttgct ggtattgcgt ggcgatctgc ttgaccgctg gcagcagcag ctacctccag
     9181 aaagcccgaa ctacttttta attaacattg ccacagaaca ggtagaaccg ctaaaagcgt
     9241 tcctcgcgga acatcagata gtcccggaat cattttatcc ggtagtgcgg gcgcggctga
     9301 cggcgattaa cgataagccg acagaaggta atgaagatga ggcgcttaac cgcgaactca
     9361 atcttacctg gcaaaatacg cgacccgatc ataatccgat tgtcgccggt aactggccgc
     9421 caaaagccga tgaagtgtcg atggaagagg ggctggcgaa acgcttaaac gttgccctcg
     9481 gtgataccgt gacttttatg ggcgataccc aggagttccg cgctaaagtg accagcctgc
     9541 gcaaagtgga ctgggaaagt ctgcggccta atttctattt tattttccct gaaggggcat
     9601 tagacgggca accgcagagc tggcttacca gtttccgctg ggagaatggc aacggcatgt
     9661 tgacacaact caaccgccag ttcccgacca ttagcctgtt agatattggc gcgattttaa
     9721 aacaggtcgg tcaggtgctg gagcaggtaa gtcgggcgct ggaggtgatg gtggtgctgg
     9781 tcaccgcctg cggtatgttg ctgttgctgg cacaggtgca ggtgggaatg cgccagcgtc
     9841 atcaggagct ggtggtatgg cgcacactcg gtgcggggaa aaaactgctg cgcacgacat
     9901 tgtggtgtga gttcgccatg cttgggtttg tttccggcct ggtggccgca attggtgcgg
     9961 aaacggcact ggcagtgttg caggcgaaag tgtttgattt cccgtgggag ccagactggc
    10021 gattgtggat tgttctgccg tgcagcggtg cgctgctgct gtcgcttttc ggcggctggc
    10081 tgggtgcgcg actggttaag ggtaaggcgc tgttcaggca gtttgcgggg tgatgagagt
    10141 aaataagggg cgttctggtt gaatggaacg ccttgtttaa attaattcta caatatgttt
    10201 cattgatgat gttattgaat tggtgatttc ctatctttct attgc
//
LOCUS       DAZGJW010000162        10144 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZGJW010000162 DAZGJW010000000
VERSION     DAZGJW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.162"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..107)
                     /locus_tag="ACHKIK_004219"
     CDS             complement(<1..107)
                     /locus_tag="ACHKIK_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
                     /protein_id="HFO9085279.1"
                     /translation="MNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHR"
     gene            complement(104..334)
                     /gene="vapB"
                     /locus_tag="ACHKIK_004220"
     CDS             complement(104..334)
                     /gene="vapB"
                     /locus_tag="ACHKIK_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HFO9085280.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLEYEKADPDFMAEREDVVSDEGRFNL"
     gene            629..919
                     /locus_tag="ACHKIK_004221"
     CDS             629..919
                     /locus_tag="ACHKIK_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085281.1"
                     /translation="MSVKITGLDKMQKQLKEVERATEALNGSYDVHFDANDPVSIENA
                     IQEAYSMVYERASGYATNPMVSPLIEHMKENLRQQILDRAEQQRQESGQDGN"
     gene            909..1808
                     /locus_tag="ACHKIK_004222"
     CDS             909..1808
                     /locus_tag="ACHKIK_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050135 - NADP+ nucleosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFO9085282.1"
                     /translation="MAIDIFQSLKNCVFDLQRADVQNYQQPLKQLARLLNSENLQSVN
                     AHLTRNVELDTFLARSEDTESSMAGSAVLQWPDEPADILGLKLLLIEKMADDNNFSFN
                     FCHTFFYDRNIIESIRKFTSSLVAPFVRDYQLYVENQHDPEPAEFRPVSRKIFIVHGH
                     DNDALQSVARFISRIGLEEIILSERPDGSRTIIEKFEAESGDVSFAIVLMTPDDSGSA
                     LASESTRLRARQNVLYELGYFAGKLGRGKVLVLRKGDIEIPSDLAGVLYTELDEHGGW
                     KRKLLSELAYAGVPFDKDKALSA"
     gene            complement(1858..4083)
                     /locus_tag="ACHKIK_004223"
     CDS             complement(1858..4083)
                     /locus_tag="ACHKIK_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HFO9085283.1"
                     /translation="MKILRQLWNQKGLDAAVEDVPEDRYGFGNIAENISRSILTLPLE
                     ASNVVGIEGAWGSGKTSLLNLILRNLALRKDAHTHVLHISPWLSGGSPVEALFLPVAT
                     VIQQEMEIRYPPKGFKKLWRKYLLSPEAQKVIEYAQDTSSRVLPLVQYIGQFSSIINW
                     IAGGIKVFSDSRLAVDQKTTTKLRAEIAGQLVSLDLKFIVVMGDLDRLEPSQVAEVFR
                     LVRAVADLPRFTHILCYDRQIITHAVEHALNIEDGSRYLQKIIQLSFKLPRPEAFDLR
                     NEFRQRAEALYQQINNQPPDSGMVRDLIAVTDTYGAALSTPREIHQAINSLIFLYPGM
                     RDFVYFPDLCLLQLIRVTNPALYDWTEHYLTERSVIEAGQGMLSDGEKADFREGLIRC
                     MKTFRASNADSFLTLADWIPGISGHNDEYLNLFEPVSEDFRHIQTTGKRLSSLTHWRY
                     YFAFSSPQNVLPPEFFRQLFEQAGVSEKQQQLSELLLSKINSVGSLSGTWFEHILSRL
                     TPGLIRERNFEECAGLVHFFFDHTDEVSTRFSIRNPWFSLREMAINEVVRHLLKHMQD
                     IDETRTITLMEKLIVTGASPFWIADFMRDLIWEHGLAQNAVPSPSDALFSRDITERLR
                     DRFAERMNQPELQQQLLLRKSLLGYLYAWRDMSSGETVKQWVREVTTTDEGLVNLLIR
                     LQTSVFSSHRGAYRRIARDQVSPFFDDWPAVEEKLKVMLSGNELTPEQEALKTALEND
                     D"
     gene            complement(4085..5173)
                     /gene="pifC"
                     /locus_tag="ACHKIK_004224"
     CDS             complement(4085..5173)
                     /gene="pifC"
                     /locus_tag="ACHKIK_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor PifC"
                     /protein_id="HFO9085284.1"
                     /translation="MLSQLNLRFHKKLIEALKTRAGRENTSVNALAERFLDDGLKTVA
                     PGDGYFQLIADPEATVRQLYRHIILGQTFGTSALSRDELRFVLVHVREAFLRGHNRLA
                     TLPALDTLLDITGNLLAWQVEHDRSVDGHYLKGIFRLAGKNWTEEFEAFRAALRPVVD
                     QMYAEHLLRPLESDCFGLAEVPDAVLAEIFTLPRLKAVFPLMLRGLDWNTEQARTLAQ
                     ELRPVISAVTETIEAGTLRLEIRVDGQPPGERPGAWYTTPRLHLLITGQDFVVPYGWE
                     ALSELLGLFTLYARHPEALTHGHQGERVMFSPPGNVTPEGFFGIDGLRIFMPVEAFET
                     LVRELATRCQEGPLAEALTGLRCLYGDL"
     gene            5725..6138
                     /locus_tag="ACHKIK_004225"
     CDS             5725..6138
                     /locus_tag="ACHKIK_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085285.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTVHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNLGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            6140..6919
                     /locus_tag="ACHKIK_004226"
     CDS             6140..6919
                     /locus_tag="ACHKIK_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO9085286.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQR
                     SEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFLMPESDAVSMLKRIP"
     gene            7099..7743
                     /locus_tag="ACHKIK_004227"
     CDS             7099..7743
                     /locus_tag="ACHKIK_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO9085287.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            7830..8138
                     /locus_tag="ACHKIK_004228"
     CDS             7830..8138
                     /locus_tag="ACHKIK_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFO9085288.1"
                     /translation="MSKAKYSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            8552..9532
                     /locus_tag="ACHKIK_004229"
     CDS             8552..9532
                     /locus_tag="ACHKIK_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO9085289.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQTVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVFPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            9525..9941
                     /locus_tag="ACHKIK_004230"
     CDS             9525..9941
                     /locus_tag="ACHKIK_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO9085290.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(9973..>10144)
                     /locus_tag="ACHKIK_004231"
     CDS             complement(9973..>10144)
                     /locus_tag="ACHKIK_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HFO9085291.1"
                     /translation="TLTFDEIQPRKNSEKLMKTLDELNQSGKGKVWFAGRGTAPEWQM
                     KREMLSQCYTTK"
BASE COUNT         2562 a         2405 c         2519 g         2658 t
ORIGIN      
        1 cggtggccgc gtagcaccgc ctgctccaga cgcttcagga cggcttctgg ctgttcacgc
       61 atgatgaacg agcagataca ggtatcaagc atataagtct tgttcacagg ttaaatcgcc
      121 cttcgtcgct gacaacatcc tcgcgctccg ccataaaatc tggatcggct ttttcgtact
      181 ctaggaatga tccccaggtt ggccggacgg ggcgcaggat gatgctgtct ccttcccgga
      241 cgatctcgag ctcgctcacc ccctcaaaat ccagatcacg aggcagacgg atggcgcggt
      301 tgttgccatt tttaaatatc gatacggttc tcatgttttt tctctcccga gcatatggat
      361 atgtgaaaca tatccggaat attcaaagca ccggtgtgtg catatgttta gtataggcag
      421 atgtgtggca tatgtatagc ggtagcatat gtagatgtca ggcatatgtt tagcatatct
      481 ctatttcttt cttttttatc ggagatccga aaaaaggtta agtcggatct taatgaaatt
      541 acatggaatt tgagtgatat cccttacccc acatctccaa cgtgctatct ataaaggttg
      601 atacatctca cttagtccgg aggttgttat gagcgtcaaa atcacaggtt tagacaaaat
      661 gcagaaacag ctaaaagagg ttgagagagc aacagaggca ttaaacggaa gttatgatgt
      721 ccacttcgat gctaacgatc ctgtcagcat tgagaacgcg attcaggaag cctattccat
      781 ggtgtacgaa cgcgcttctg gttatgccac taatccgatg gtcagcccat taatcgaaca
      841 catgaaagaa aatctgcgcc aacagattct ggacagagca gaacaacaaa gacaggagag
      901 cgggcaggat ggcaattgat atctttcagt cacttaaaaa ttgcgtattc gaccttcagc
      961 gcgctgacgt tcagaactat cagcaacctc ttaagcaatt ggcccggctc ttgaactcag
     1021 agaatctgca gtcagttaat gcacatctga cccgcaatgt tgaacttgat acctttctgg
     1081 ccaggagtga agatactgaa tccagcatgg ctggttccgc tgttctacag tggcctgatg
     1141 agcctgctga tattttgggc ctgaagttgc tgctgatcga gaaaatggca gacgataaca
     1201 atttttcctt taatttctgt catacatttt tctatgatcg aaacattatt gaatccattc
     1261 gaaaattcac gtcgtcatta gttgccccgt tcgtgcgcga ttaccagttg tatgtggaaa
     1321 accaacacga tcctgagcct gcagagtttc ggccggtttc ccgaaaaatt tttattgttc
     1381 atggccatga taatgatgcc cttcagtctg ttgcccgttt tatatcccgg ataggtcttg
     1441 aggaaatcat tctttcagaa cgtcctgacg gctccaggac gattatcgag aaatttgaag
     1501 cggagtcagg ggatgtcagt tttgccatcg tgctgatgac gcccgacgat agcggcagtg
     1561 ctctggcatc tgaaagcaca cgtctccgcg cacgccagaa tgtcctgtat gagctcggat
     1621 atttcgccgg gaagcttgga cgcgggaaag tacttgtact caggaaggga gatatcgaaa
     1681 tcccttcaga tcttgccggg gttctctata ccgagcttga tgagcatggt ggatggaaac
     1741 gcaaactgct gagtgagctt gcgtatgccg gtgttccttt tgacaaggat aaagcattaa
     1801 gcgcctgatc cccttcattt agtaagaaaa aggccgctaa gcggccttaa tttttggtca
     1861 gtcgtcattt tccagcgctg ttttgagcgc ttcctgctcc ggagtcagct cgttgccgga
     1921 cagcataacc ttcaacttct cttcgactgc tggccagtca tcaaagaacg gactgacctg
     1981 gtcacgcgca atccggcgat atgcccctcg gtgggagctg aagacactgg tctgtaatcg
     2041 tatcaacagg ttaacaaggc cttcatcagt ggtagtaact tctctgaccc attgcttcac
     2101 ggtttcacct gaactcatgt ctctccaggc gtagagatac ccgagaagag acttgcgcag
     2161 gagaagctgt tgctgaagtt ctggttgatt catacgttcc gcaaacctgt cacgcaaccg
     2221 ttccgtaata tcgcgactga aaagagcgtc tgaaggcgaa ggtactgcat tctgagcaag
     2281 tccatgttcc cagataagat cccgcataaa atcggctatc cagaacggcg aagcacccgt
     2341 aacgatcagt ttttccataa gcgtgatcgt tctggtctca tcaatatcct gcatatgctt
     2401 cagcagatgc cgtacaacct cattaatggc catttcacgc agggaaaacc atgggttacg
     2461 gatactgaag cgagtgctca cctcatcggt gtgatcgaag aaaaaatgaa ccagtcctgc
     2521 gcactcttcg aaattacgtt ctcttatcag gcctggagtc agtctgctca gtatatgttc
     2581 aaaccaggtt ccggacagac tgcccacact gttgattttt gagagcagca attcagaaag
     2641 ctgctgctgt ttttccgata cgccagcctg ctcaaaaagc tgtctgaaga attcaggtgg
     2701 cagaacattc tgtggtgagg agaaggcaaa ataatatcgc cagtgcgtca ggctgctgag
     2761 tcgtttaccg gtcgtctgga tatgacgaaa atcctcactg acgggctcaa aaagattcag
     2821 atattcatca ttatgtccac tgatacctgg gatccagtct gcaagtgtca gaaacgagtc
     2881 tgcatttgat gccctgaacg tcttcataca tctgataagc ccctcccgga agtctgcttt
     2941 ctctccgtca gaaagcatac cctgaccggc ttcaatcacg gaccgttctg tcaggtaatg
     3001 ctctgtccag tcatacagag ccgggtttgt cacccgtata agctgaagca ggcacaaatc
     3061 agggaaataa acaaaatccc gcatccccgg ataaagaaaa atcagggaat taatggcctg
     3121 atggatttcc cgtggcgtcg aaagtgcggc accataggta tcagtcaccg cgatgagatc
     3181 ccttaccatt ccagagtctg gcggttgatt attaatttgc tgatatagag cctcagcccg
     3241 ctggcgaaat tcattacgta aatcaaaggc ttcaggtcgg ggtaatttaa aactaagctg
     3301 aatgattttc tggagataac ggctgccatc ttcgatattc agcgcatgtt caacggcatg
     3361 agtgataatc tgcctgtcat aacagagaat atgggtaaag cggggcagat cggctactgc
     3421 acgcacaagc ctgaacactt ccgccacctg ggatggctcc agtcggtcca gatcacccat
     3481 gacaacaatg aacttcagat ccagactcac cagttgtcct gcaatttcag cccgaagctt
     3541 tgtcgtggtt ttctgatcaa cagccagacg gctgtctgaa aataccttta ttccgcctgc
     3601 tatccagtta ataatgctgg aaaactgtcc gatatattga accagtggaa ggacgcgcga
     3661 tgaagtatcc tgagcatact caatcacttt ttgagcttcc ggtgacaaca gatatttacg
     3721 ccagagcttt ttgaagccct tcgggggata gcgtatttcc atttcctgct ggataaccgt
     3781 ggcaaccgga agaaagagcg cttcaaccgg actgccgcca ctcagccagg gagaaatatg
     3841 cagcacatgc gtgtgagcat cttttctcag ggcaaggttt ctcaggatca gattaaggag
     3901 gcttgtttta cccgatcccc atgcaccttc tattccgaca acatttgaag cctccagagg
     3961 aagagtaagg atggaacggg atatattttc tgcgatatta ccgaaaccgt atcgatcctc
     4021 aggtacatct tcaactgccg catcaagccc tttctggttc cacagctgcc tcagtatctt
     4081 catattacag atctccgtac aggcagcgca gcccggtcag ggcctccgcc agcgggcctt
     4141 cttgacaccg cgtggcgagc tcccggacca gcgtctcaaa ggcctccacc ggcataaaaa
     4201 tccgcaggcc gtctatcccg aagaagccct ccggcgtgac gtttccgggc ggggagaaca
     4261 tcactcgctc cccctggtga ccgtgcgtca gggcttccgg atgccgggca tacagcgtga
     4321 acaggcccag cagttcggat aacgcttccc agccgtacgg caccacgaag tcctggccgg
     4381 tgatcagcag gtgcagacgg ggtgtggtgt accaggcccc cgggcgttct ccggggggct
     4441 ggccgtcaac gcggatctcc aggcgcagcg tgccggcctc aatggtttcc gtgacggcag
     4501 aaataaccgg gcgcagctcc tgtgccagcg ttcttgcctg ttctgtgttc cagtccaggc
     4561 cgcgcagcat caacgggaaa acagctttca gtcgcggcag ggtgaagatt tctgccagca
     4621 ccgcgtccgg cacctccgcc aggccaaaac agtcgctctc gagcggacgc aggaggtgtt
     4681 cggcatacat ctgatccacc acggggcgca gcgctgcccg gaaggcttca aactcttccg
     4741 tccagttttt cccggccagg cggaaaatcc ccttcaggta gtgaccgtcc acagagcgat
     4801 cgtgctccac ctgccaggcc agcaggttgc cagtgatgtc cagcagcgtg tccagggccg
     4861 ggagtgtggc cagcctatta tgcccgcgca ggaaggcctc cctgacgtga accaggacaa
     4921 agcgcagttc gtcgcgggag agcgctgacg tgccgaaggt ctggcccaga atgatatgcc
     4981 ggtacagctg ccggaccgtg gcctccggat cggcaatgag ctgaaaatac ccgtcaccgg
     5041 gcgcgaccgt tttcaggccg tcatcgagga agcgttcggc tagggcgttg accgaagtat
     5101 tttccctacc ggcacgcgtt ttcagcgcct cgataagttt tttgtgaaaa cgcaggttaa
     5161 gctggcttag catcatcgat cctttcatgt ttgttttgtt cttaatgcta acctagcatt
     5221 ttattattgg ctattaatca gttttttagt ttttgaaaat tgtgatgctt ctaaaattac
     5281 taaaattgag gattttaatg ctacaacaat gcctgcctct tcttatttct ccggagatcc
     5341 gaaaacccca agttcaggag cttcctctcc ccccacacaa cgccacctcc tgtaagcaca
     5401 acatgtggtg ctgcattaag ctgctgatga cactatatgt tgtgttattt cccttgcctg
     5461 tgatgcgccg cgcaggggcg gaaaacagcg atatgatgat ttcctcagcg tggtacactt
     5521 ccggaaagtt gtgatattcc ggaaagtcgg atctgacgga aacagctctc cggtaattta
     5581 acggcgtggt tatatggatg cttgttatca tggtaatgat gataacggca tgatgttatc
     5641 agatggcgtg acggtaaggg cagtgatgat ggatgacgtt atcgcatgac cgtccctgcc
     5701 cggaaaagaa aaaaggagtc acccatgttt tttattgaga atgaaggtca ggctgtcgcc
     5761 agaacggatt actggcagtc tgtacaggcg caggccggat atgtctacct cagctggaat
     5821 gccggcgcag ccaggctgct tgtcccggat gcggcaaaac atttactcag ggagatgcgg
     5881 ggagctgagt acgtcatcat cagtaaggga acggtgcatg gccgcgatgc gctggaactg
     5941 gtatttgaag acggcagcga tgcgccgttc gtgatccaca tgctgagtga gcagtgcgat
     6001 cgcctgctcc ccgaaaacaa cctgggaggg ggttttgttg tcaccgtctg gacgcgtggc
     6061 ggtaaccagc tccgttatcc gggaaagtac cgggttgtgg aaaacctgcc cgacgtttcc
     6121 ccgtggagtg aacactgata tgcagcacct gccggcaccg atccaccatg cccgggatgc
     6181 cgctcagctt cctgttgcca tcgattatcc ggcagcgctg gcactccgcc agatgtcgat
     6241 ggttcatgat gaactgccaa aatacctgct ggcccctgaa gtgagcgccc tgctccatta
     6301 cgtcccggac ctgcgccgca agatgctgct ggccacactg tggaacaccg gtgcgcgcat
     6361 taatgaagca ctggcgctga cgcgggggga tttttcgctt gcgcctccgt atccgtttgt
     6421 gcaactggcc actctgaagc agcggtcaga aaaagccgcc aggacggcag gaagaatgcc
     6481 cgccggtcag cagactcacc ggctggttcc gctctccgac tcctggtacg tcagccagct
     6541 gcagacgatg gtggccacac tgaaaatccc catggaacgg cgtaataaac gaacaggcag
     6601 gacagagaaa gcgcggatct gggaagtgac ggacagaacg gtcagaacct ggattgggga
     6661 ggcggttgcc gccgctgccg ctgatggtgt gacgttctct gtcccggtca cgccacatac
     6721 gttccgccat tcctatgcga tgcacatgct gtatgccggt ataccgctga aggtcctgca
     6781 gagcctgatg gggcataagt ccatcagctc aacagaggtc tacacgaagg tgtttgcact
     6841 ggatgtggct gcacggcacc gggtgcagtt tttgatgcct gagtccgatg cggtgtctat
     6901 gctcaaaaga attccataag ctctgacttt tttaaatccc tttcggatat ctcatggata
     6961 tattatcggt atttcatatt ctgttgacat gcaggaccgg tatagagtaa aaaaacatct
     7021 attgagtacc tattggatat ccgaaaggtg tcttaggggg tctttatggg tattacatgt
     7081 ggattctgga gatacactat gcctgttatt gctaatacgc atcccaaagg tggtgtgggt
     7141 aagacaactt catccgtaaa cattgttggt gaaatgaagt ctgatacagt tgatctggat
     7201 actcataccg ggctttctat cattctgggg ctgagacctg aaggaaaaga aatttccgtg
     7261 aaagtaccca aaacagtgga tgagttaatc gaaattatga ctccctacaa gaacagcgat
     7321 aagacgttac ttattgactg tgggggattt gactcagatc ttacccgtac tgcgattgcc
     7381 tttgcagact gcgttattgt cccttcaaaa gactccctga ctgaacgtat tggtttgatg
     7441 cattttgatg gagtactgga cgaaatcagc tctatcatgg ggaccgatat aactgctcac
     7501 ctgtatctct gtaaagttaa tcctaacaag aagaaattcc ctaagcttga tgcaatcctg
     7561 ccatcgttca aacatctgaa gctgatgaag agccgcattt ctgctcgtgc agaatttgag
     7621 gatgtcattg agactgggat gggaatcact gagtctgttc atgggcgcta ttcggcagga
     7681 ggtaaggaag ttattgccct gatcgaagaa atcaatcatc tgattgaaaa taacaaacag
     7741 taaggtgtgt ctattgggta tccatatagt atcctttggg tatttaatgg atatctttta
     7801 agtatctaac aaacatcctg ggttatctca tgagcaaagc gaaatatagt gcacaggctg
     7861 aaaaactgaa aaacctggtg gattctgcca gattacaaac ttcttcttct gccgtaacag
     7921 caacagcccc atctcagcca aatgaaggtg cagaggaagt ttcaggtcgt cccgtggctg
     7981 ccaggacgaa ggctaagaat cttaaggcca taccgctgtc gtattttgaa gctcatgcgg
     8041 aacttaaagc gaccagtaaa acatctctgg attttagctc atatattatt gaggctataa
     8101 gagagaagct tgaacgtgat ggtgcaatag gtcaataaac tatcggatac cttaagggta
     8161 tctgatgtgt gtcttttaaa tgcgttatgg ggattcttca cgtacctaaa gagtccctat
     8221 tagacatctt tctgtttatg gtgacatgat tcgtcaccgc tttcctttcc tctcagtttc
     8281 accatttcat tccttttccc ttaacctgaa ccatgatgtc aaagatgttt ttatattgta
     8341 tttatgtatc acatatagcc ttcataatgt ttttgtatct atgatttcct ctggtcttaa
     8401 agttgtgtgt acaatttaca tgcaatttat cgtgttttta tgtcgcattt gtcctttcag
     8461 aaagaccatt ttttcattat aattgaacat acattgcatg tatgaagcat gtttgttttt
     8521 tctatttgtt tatgagcaac ggagactggt tatgaaaatc tttattgatg atggttctac
     8581 caacattaaa cttgcctggt tggaggatgg tgacgtgaaa acgttaatca gccctaacag
     8641 ctttaaaccg gaatggtctt ttagtttact ggatgatgct gccccggcta attacgagat
     8701 cgacggcgag aagttttcat ttgatccatt aagtgctgat gctgtcgtaa cgactgaaac
     8761 acgctatcag tacagtgatg tgaatgttgt ggccattcag catgcgctac agcagaccgg
     8821 cctgaaagcg cagactgttg atgtcatcgt cacattgccg atctctgaat accttgacgc
     8881 caacaatcag aaaaataagc aaaacattga aagaaagaaa aaaaatgtga tgcgtgaagt
     8941 acgggtgcag gggagtgatg catttgttat tcgcagtgtt tccgtttttc ctgaaagtat
     9001 tcccgctggt ttcagtgttc ttgcggggct tgaggatgat gaatcccttt tgatcgtaga
     9061 tctcggtggt acaactcttg atgtgtcgca tgttcgtagc aaaatgacag ggattacaaa
     9121 aacctggtgt gatccgaata tcggcgtgtc cctgattaca tccggggtta aggaacagat
     9181 ggctgtacat gctaatacaa gagtgagctc attccaggct gacaatatca ttgtgcaccg
     9241 taatgaacct gactaccttt cccgtcgcat ttataatgct gaacagcgtg agtctatcat
     9301 taatgttatc aatgaacggc agaagttgct tattaaacga gttaatgatg tcatcagccg
     9361 tttcaccgac tatacacatg taatgtgtgt tggtggtggg gctgaaattg tggccgaggc
     9421 tgtgaagaac ctgactaagg ttcctgatga gcgcttttat ctgagctcat ccccgcagtt
     9481 tgatttagtc atgggaatga taaaaatgaa aggtggtgtg actaatgagt gatgaaaata
     9541 aatccagacg ctgctcattt gaacttttcc cggatgaacg gacaggagat aagatagcag
     9601 atgagctcat tgcaaatgaa aaactgaagg agcggggacg tttcatgcgc gcgatgttag
     9661 ttactggtgc ggcgtttgca gctatagata aacgtcttcc tttgctgata tcggagttac
     9721 tgacagaaaa tacgactctg gatgatatta ataaggtgat ttccagcgtg ataccaggtg
     9781 ctttttcggt tgaaaagaaa ttgcttgaat tactggaaaa acaatctggt ctccatacgt
     9841 ctgtggattg cagtactcct cttacagagc agagtctgtc gagaaatgac ggtgaagacc
     9901 agaccagaag aaacgctgaa aatatgttcg gagatgactg aactaaccca gcctggccag
     9961 gggaatatct cgctatttag ttgtataaca ctgactcaac atttcccgtt tcatttgcca
    10021 ttcaggggcg gttcctcgcc ccgcaaacca cactttccct tttcccgact ggttcagttc
    10081 atcgagtgtt ttcattaact tttcactgtt tttacggggc tggatttcat caaatgtaag
    10141 cgta
//
LOCUS       DAZGJW010000163        10075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZGJW010000163 DAZGJW010000000
VERSION     DAZGJW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.163"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(35..652)
                     /gene="yncG"
                     /locus_tag="ACHKIK_004232"
     CDS             complement(35..652)
                     /gene="yncG"
                     /locus_tag="ACHKIK_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO9085292.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIAFMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNEII"
     gene            918..2417
                     /gene="ansP"
                     /locus_tag="ACHKIK_004233"
     CDS             918..2417
                     /gene="ansP"
                     /locus_tag="ACHKIK_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO9085293.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2532..3593)
                     /gene="yncE"
                     /locus_tag="ACHKIK_004234"
     CDS             complement(2532..3593)
                     /gene="yncE"
                     /locus_tag="ACHKIK_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO9085294.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3835..5937
                     /gene="pqqU"
                     /locus_tag="ACHKIK_004235"
                     /pseudo
     CDS             3835..5937
                     /gene="pqqU"
                     /locus_tag="ACHKIK_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
     gene            complement(5973..6638)
                     /gene="mcbR"
                     /locus_tag="ACHKIK_004236"
     CDS             complement(5973..6638)
                     /gene="mcbR"
                     /locus_tag="ACHKIK_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO9085295.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6836..7873)
                     /gene="curA"
                     /locus_tag="ACHKIK_004237"
     CDS             complement(6836..7873)
                     /gene="curA"
                     /locus_tag="ACHKIK_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO9085296.1"
                     /translation="MRQQKQRNRRWVLASRPHGAPVPENFCLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8054..8572
                     /gene="mddA"
                     /locus_tag="ACHKIK_004238"
     CDS             8054..8572
                     /gene="mddA"
                     /locus_tag="ACHKIK_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO9085297.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8569..9018
                     /gene="ydcZ"
                     /locus_tag="ACHKIK_004239"
     CDS             8569..9018
                     /gene="ydcZ"
                     /locus_tag="ACHKIK_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO9085298.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(9019..9252)
                     /gene="ydcY"
                     /locus_tag="ACHKIK_004240"
     CDS             complement(9019..9252)
                     /gene="ydcY"
                     /locus_tag="ACHKIK_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO9085299.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9338..9511)
                     /gene="ortT"
                     /locus_tag="ACHKIK_004241"
     CDS             complement(9338..9511)
                     /gene="ortT"
                     /locus_tag="ACHKIK_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO9085300.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9706..9801
                     /gene="yncL"
                     /locus_tag="ACHKIK_004242"
     CDS             9706..9801
                     /gene="yncL"
                     /locus_tag="ACHKIK_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO9085301.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2433 a         2459 c         2451 g         2732 t
ORIGIN      
        1 gaataataaa taacataatc ttctggtatc ccactcaaat aatttcattc ctttttaata
       61 cttcttgtaa tttcggtaac tgacatacag catcagcgat cgcgctgata ttctgcgcat
      121 tatcctgaaa ccattcatgc cccggccccc atgtgcgcat ggtgcagaga taacagtcca
      181 ccagcgttag ttgctcacca aaggcataag gttcagcggt caattgtgaa ttcagccaga
      241 tataaagtga tttgcgatat tcaattacat tcttctttaa ctgttcaggt gcgtcagatg
      301 cccaacgctc agggtaatcg gcgaaagtga atgttggata gacattagca accagccaga
      361 ccaatagccg ttgaaactgc tgacgttcgg cgcgcccaac cggtggcgca aggtccgggc
      421 gacgatcgag aaccataaat gcaatcgccg ctgtctccgt cataatctcg tcattttcca
      481 gcgccagtgt cggaacctga cacaacgggt tcagggtttt caataactcg cgtgaagctc
      541 cctcgtggtc aaaaccactg acatcaacga actgataagg aatatcagcc agtgttagca
      601 tcagctcact gattgtagag ccccatccgg gtacgccata caccttaatc atatgccctc
      661 acaatctttt aaagattaag tgtaggacag tacacaggtt aaccagcaaa aagatttgtc
      721 attttttcgt cagttgtatt attccccttc aggattgtaa ggggatgttt tgcatcgttt
      781 atcgataacg ggtaggatgc tgcgccgata gttttatacc cataaagaat aatggtgata
      841 actatcatcg ccaggatgaa taaacattgt tcatggcaac ttatatgact ttttcattaa
      901 agcaatcagg gagagcaatg agtaaacacg acaccgacac ttcagatcaa cacgccgcga
      961 aacgccgctg gcttaatgcc cacgaagagg ggtatcacaa agcgatgggc aatcgccagg
     1021 tacagatgat cgccattggc ggcgcgattg gcaccggctt gtttttaggt gcaggagccc
     1081 gactgcaaat ggcgggtcca gcattggcac tggtttattt aatttgtggc ttgttttcgt
     1141 tttttattct gcgtgcattg ggtgagctgg tgctacaccg cccttccagt ggcagttttg
     1201 tctcttatgc ccgtgagttt ttgggtgaga aagccgctta tgttgctggc tggatgtact
     1261 tcatcaactg ggcgatgacg gggattgttg atattaccgc cgtcgctctg tatatgcatt
     1321 actggggtgc gtttggcggc gtgccgcagt gggtctttgc gctcgctgca cttaccatcg
     1381 ttggcaccat gaatatgatc ggtgtgaaat ggtttgcgga gatggagttc tggtttgcgc
     1441 ttattaaagt gctcgccatt gtgacctttt tggtcgtggg tacagtgttc ctcggtagtg
     1501 gtcagccgct ggatggcaac accactggct ttcatttaat taccgataat ggcggcttct
     1561 tcccccacgg tttgctgcct gctctggtgt tgattcaggg cgtagtgttt gcttttgcct
     1621 ccattgaaat ggtgggtaca gctgccggag aatgtaaaga tccgcagacc atggtgccta
     1681 aagcgattaa cagcgtgatt tggcgtattg gcctgtttta cgtcggctcc gtggtgttgc
     1741 tggttatgct attgccgtgg agcgcgtatc aggcggggca aagtccgttc gtgacgtttt
     1801 tctctaaact gggtgtgcca tatatcggca gcattatgaa cattgtggtg ctgaccgctg
     1861 ccctctccag cctgaactca ggtctgtact gcaccggacg tattctgcgc tcaatggcga
     1921 tgggcggttc cgcaccgagt tttatggcga aaatgagtcg tcagcatgtg ccgtatgccg
     1981 ggattctggc gacactagtt gtgtatgtcg tcggcgtatt cctcaactat ctggtgccgt
     2041 cgcgcgtatt tgagattgtg ttgaacttcg cgtcgctggg aatcatcgct tcatgggcgt
     2101 ttatcatcgt gtgccagatg cgcctgcgta aagcaattaa acaaggcaaa gcagcggatg
     2161 tcagttttaa actgcctggc gcgcccttca cttcctggct gacattactg tttttactga
     2221 gtgtcctggt gctgatggcg ttcgattacc cgaacgggac ttatactatc gcggcgctgc
     2281 cgattatcgg tattctgctg gttataggct ggtttggtgt gcgcaaacgc gttgctgaaa
     2341 ttcacagcac tgcgccagtc gtcgaggaag atgaagaaaa acaggaaatt gtgtttaagc
     2401 ctgaaacggc gagctaattg caaaattgcc cggctacgtg ccgggcaaat ttagattact
     2461 gacgaaggtg tgccgcatgc ggcgtgataa aagtcaggca agtctctccg ttttcaccct
     2521 cgccacatct attacagcgc aatacgaatc acatcgtccg gctgggtagc ttcctgctgt
     2581 ttagtggatt tttgtttcac actgacatac agcgttttgc catcggcaga cagcgccagg
     2641 ctgtttggat gagtcggcgt atcgaacgtt ttcaccactt tatagctttt cgcgtcaatc
     2701 acactgactt tacctgcctg acgatgcgtt acgtaggctt cattacgcgc tgggttaaac
     2761 agcacagcca gtgattctgg tgccgcaacc ttcgccagaa tattgccatt acgggtatcg
     2821 accactaaca cttctgcggc tttagaatcg gtgataaatg cacgctgcct ggcggtatca
     2881 aggctaatgt tgataaagaa gtgctctttg ccgtcatcca gcagcttttt acggctgagg
     2941 attttattgt cggcggtgtc gatggtaatc aattcgccgt cagcgttagt ggtgtaaaga
     3001 cgtttgcctt tgctatccag cgccagaccg gtactcattt taccggtgtt ctggatggcg
     3061 gttttcagtt tgatattctc gccatcaacg acccaaatca cgctctcttt accaatacca
     3121 ctgatgtaaa cggtgttcgt ggcatcatca gctaccagct cacgcggttg cagcgggcgc
     3181 acctcttccg tgcgcttacg atcatccagc accagacggc ctttcacctc gcccgttttg
     3241 gcatctatcg ccgtgaccgc gctgtttacg gtgttaccaa accacaacgt ctgagtcgtg
     3301 ttattgatgg tggcaccaaa cggcttgaga tcgttatgga tcgcctgcgt cacttccaga
     3361 gtgaccggat caagacgata aaccacgcca cctttatcca gtttgcggct ttgcgaagtg
     3421 gcgagccaca gtgcgttttc ttgctggcta taagccattt cgtaggcacc tttacctacc
     3481 gctttacgca gcatttcttc tgcggcctgc gtactgaatg atgaagcaac aagcaatgaa
     3541 cctaacagta atgaaccacg caggcgcgat gaaaacagat gacgtaaatg catgacgact
     3601 ccctttgatg aaccgatatt tttgctgctt cacaatgctt cgctaagcgt aatcatcgtt
     3661 acgctcttgt tataaatggg aatggtaatc attattttct caaatgtgga gcatttcttg
     3721 ccgttacgac tacaattttc tcaattactt aacgtatcgt gccgtttcgt tttccataaa
     3781 tttacaattt cattaccata acggcgtttt cccattctta acctggcttt tttcatgaag
     3841 attttttccg tccgacagac cgttttgccc gcactgcttg tcctttcccc cgttgttttt
     3901 gccgctgatg aacagactat gattgtcagt gccgcaccgc aggtggtttc agaactggat
     3961 accccagcag cagtaagcgt ggtggatggc gaggagatgc gcctggcaac accgcgcatt
     4021 aacttgtccg aatcactgac cggcgtgcct ggtttgcagg tacaaaaccg gcagaactat
     4081 gcgcaagatt tacagctgtc gattcgcgga tttggctccc gctccactta cggtattcgc
     4141 ggtattcgcc tgtatgtgga cggtattccc gccaccatgc ccgacgggca agggcaaaca
     4201 tccaacatcg atttaagcag tgtgcaaaat gtggaagtgc tgcgtggccc cttctctgcc
     4261 ctgtatggca acgcgtctgg tggggtaatg aatgtcacca cccagaccgg acaacagcca
     4321 ccaaccattg aagccagtag ttactacggc agttttggca gctggcgcta tgggctgaaa
     4381 gcaacgggcg caacgggaga cggcacacag cctggcgatg tcgattacac cgtctcaacc
     4441 acgcgtttta cgacccacgg ctatcgtgac catagtggcg cacagaaaaa tttagctaat
     4501 gccaaactgg gcgtacgcat tgatgaagcc agcaaattaa gtctgatttt caatagtgtg
     4561 gatatcaaag cagatgaccc aggtgggcta accaaagcag aatggaaggc taatccacaa
     4621 caagcgcctc gtgcagaaca gtacgacacg cgaaaaacca tcaagcaaac tcaggctggg
     4681 ttgcgctatg agcgtagcct gagcgcgcaa gatgatatga gtgtgatgat gtatgccgga
     4741 gagcgagaaa cgacccagta ccagtcaatc ccgatggccc cccaacttaa cccgtcacat
     4801 gcgggcggcg tgattaccct gcaacgccat taccagggga tagacagccg ctggacacac
     4861 cgtggtgaac tgggcgttcc ggtcacgttc actaccggcc tgaactacga aaacatgagt
     4921 gaaaaccgca agggctacaa taacttccgc ctgaatagcg gcatgccgga gtacgggcaa
     4981 aaaggtgagt tgcgtcgcga cgaacgcaat ctgatgtgga acatcgatcc ctatttacag
     5041 acgcagtggc agctgagcga aaaactgtcg ctggatgctg gcgtgcgcta cagctccgtg
     5101 tggtttgatt ccaacgacca ttacgttact ccgggtaacg gcgatgacag cggtgatgcc
     5161 agttatcata aatggctacc tgccggttcg ttaaaatatg caatgaccga tgcctggaat
     5221 atctatctgg cagccgggcg aggttttgaa acgccgacga ttaatgagct gtcttatcgt
     5281 gctgatgggc aaagcggtat gaactttggt ttaaaaccat ccaccaacga tacaattgag
     5341 atcggcagta aaacgcgtat tggtgatggg ctgcttagtc tcgcattgtt tcagaccgac
     5401 actgatgatg aaattgttgt cgatagcagt agcggtgggc gtacgactta caaaaatgcc
     5461 ggaaagaccc gtcgtcaagg cgctgaactg gcatgggatc aacgtttcgc aggagatttt
     5521 cgcgtaaacg cgtcctagac ctggcttgat gcgacctatc gcagcaatgt ttgcaatgaa
     5581 caggattgta acggtaatcg gatgccaggg atcgcccgta atatgggctt tgcgtcgata
     5641 ggttatgtac cggaagatgg ttggtatgca ggcacggaag cgcgttatat gggcgatatt
     5701 atggcagatg atgaaaatac ggcaaaagcg ccgtcttata ctctcgtcgg cttattcacc
     5761 gggtataaat acaattacca caatttaact gtggatttat ttggtcgtgt cgataattta
     5821 ttcgataaag aatacgttgg ttctgtcatt gtcaatgagt caaacgggcg atattacgaa
     5881 ccttcgcccg gacgaaatta tggtgtcggc atgaatattg cgtggagatt tgagtaatta
     5941 ccttgatgcc cggtatttgc cgggcattta ctttaacgat tgtattgctg gtataaaata
     6001 gcaatatttt gttgcataat ttcagcaaga caatgtctgc atccctcttt gtcttttgct
     6061 tttaacgcag cgagtaacag atgatagttt tctatatgct cccgtaattc cgctggatta
     6121 atcgcttcat agagataatg taaaccaggc cccatcctga cccacagttg ctcaatcatc
     6181 tcacacagga tgggcatatt tgagcgatga taaatcgcta agcgaaatag cctgtttaca
     6241 ttaatgattt gttccatgtc gcccttttct tgcgcctgct gtaatttctc gagcaattcc
     6301 tggagttccg ccaggtcttg cggggtgagg ttttcaacag ccagagcaac tgccattaat
     6361 tccagttcgt agcggatccg attgatttcg tctaattgac gtttcctcac ttccggaact
     6421 gtaaatgctt gtgcaggtgc gacagaaagc gcattcaccg aaaccagacg taataatgct
     6481 tcacgcacag gtgtaatact catacctaat tgctccgcca gatttttagt aatcaggcgt
     6541 gcgccaggtt ttaatgcgcc aatactaagc tgatgtttca ggtcgttctc aacctgcaga
     6601 gtcaaactga catgtttcat ttttcccatt ccaggcattt taattccttc gcgtgttttc
     6661 tgtccaaccc tgcctgcacg gcagaaggag ttcacctggt gtattaaagt gaataatcat
     6721 aaaccaccca cctttgttgt tattagcagc agaacaatga cgtggttagt taattaaagc
     6781 tgaaaataag ctttttgcct ggatttttat ggcggcttac gccgccgttg aaactttaat
     6841 catcacccgc gacgcggatc accactttgc cgaagttttt acccttcagc aggccgataa
     6901 acgtctgtgg cgcattctct aaaccatcag taatatcttc gtggtagtgg attttatcct
     6961 ctttcaccca ttgccccatc tccttctgaa actcatggat gcggtgacca taatcctgag
     7021 cgataataaa cccttgcaag cgaatacgtt ttttcagcac cgttgccatc aacaggggta
     7081 aacgatccgg accgggtggt agctctgtag cgttatagct gctcactaag ccacaaacgg
     7141 gaatgcgcgc agatgtatta agtaatggta gcactgcatc aaatacctta ccgcccacgt
     7201 tttcataata gatatcaata ccttttgggc acgctttcac cagttgttcg gcaaaatcat
     7261 ccgcgtggtg atcaagacaa acatcgaagc ccagcacctc gatagcatgg cggcattttt
     7321 ccgcgccacc ggcaaccccc accaccctgc aacctttaag tttgccgatt tgccccaccg
     7381 ttgctcccac aggacctgtc gccgcagcta ccaccaacgt ttcgccctct ttaggctgac
     7441 cgatatccag cagccccata taagcggtaa agcctggcat ccccagcaca cccagcgacc
     7501 acgatggatt ttgcggatga tcgccaagtt tcaccagatc atcaccactg gatatgtcat
     7561 agtcctgcca tccactgtag cccagcaccc agtcgccagg ctgataatca ggatgattcg
     7621 actccacaac acggctcacc gtgccgccga ccatcacgcc gccaatatca acaggcggtg
     7681 aataagatgg ctcatcgctc atacgtccac gcatatacgg gtccagggac aaataaactg
     7741 tgcgcagtaa cacctgtcct tcacccggtg tggcgacatc atcttcttca agacagaaat
     7801 tctccggaac cggtgcgcca tgtggacgcg aggccagaac ccaacgtcga ttacgctgct
     7861 tttgttgcct cataatgctc tcctgtgctt ttggcatgaa tccatcaata gtagcaggct
     7921 cacgggattg gttttgtttg cagccgtcac atcccctctg caacacgcaa taatttcatc
     7981 gaggaatccg ttgcttcggg ctgttttcga tagtattgat aacatcatca tcattatttt
     8041 atcgaggcca gacatgtcca tccgttttgc ccgcaaagcc gattgtgctg ccattgcgga
     8101 aatttataac cacgccgtgt tgtatacggc ggctatctgg aatgaccaaa cggttgatgc
     8161 agataaccgc attgcctggt ttgaagcgcg gaccatagca ggttatccgg tgctggtgag
     8221 tgaagaagat ggcgtagtga cgggatatgc ctcgtttggc gactggcgta gtttcgatgg
     8281 ttttcgccat accgtggaac attcggttta tgtccatccc gatcatcagg gcaaaggtct
     8341 ggggcgtaaa ttgttaagcc gattgattga tgaagcgcgg gattgcggga agcatgtcat
     8401 ggtcgccggg atcgaatcgc aaaatcaggc ctcgctgcat ctgcaccagt cgctgggatt
     8461 tgtcgtcacc gcacaaatgc cgcaggtggg cactaaattt gggcgctggc tggatctgac
     8521 atttatgcag ttgcaactcg acgaacgcac tgaaccggac gcgattggat gaatcagtcg
     8581 ctcacccttg cttttttgat tgccgccgga attggtctgg tagtccagaa cacgctgatg
     8641 gtacgcatca cccagacctc ctctaccatt ctcatcgcca tgttgctgaa ctcactggtg
     8701 gggattgtgc tctttgtttc tattttatgg tttaaacaag gcatggcggg gtttggcgaa
     8761 ctggtgtcca gcgtgcgctg gtggacactt atacccggct tgctgggatc gttttttgtc
     8821 ttcgccagta tcagcggata tcagaatgtg ggagcggcga caactattgc agtactggtc
     8881 gccagccagc taattggcgg tctgatgctg gatatattcc gcagtcatgg cgtaccacta
     8941 cgggcgttgt tcggcccaat ttgtggcgca attttacttg tggttggcgc ctggttggta
     9001 gccagacgct cattttaatc agaggatggt gccgcctttg gttaactgtt cgtggcgcgc
     9061 ttccatatct tctttatgct tgcgaccgtg atgagcaata gcggtgcgca aacgctgctg
     9121 ctgggtgtag cgatcttccc gacttaactc cgcgtcatcg ctcagggcga ttaataattc
     9181 gttcatatgg gcaatgacgc tctcttcaat cgcggcatcc acgcgcgcga tgacttcatc
     9241 cagatgcgac atactttctc cttaaccact tgtgaaattt atcgattcat ccaaatgata
     9301 taagtctgcg tggtgccgtc aagccagtga gtaagattta aaacagtgaa aacaacagcg
     9361 ccaccgggaa actcatcggc catgttgccc ccaccagaaa ggcgcttaag aaacggatgc
     9421 gtttcttatc gtgagaaaga aaccaggtga taagaaatgc gattgctgcc ataaccgcat
     9481 aaaaaacaag catatgttga tagagagaca tgtacgggcg tccagccaga tgaataaaac
     9541 gcgcgcaaat atgcgtcatt ttttgacgtg catcaagttt tttcaccagt ttgagaaaaa
     9601 atcatcaaac agcaacttgc ccttatccat cttcagaatg agtattattg cggattttct
     9661 taacccgtac tatacacata ggggcaatga taaaaggtgg caaaaatgaa tgtttccagt
     9721 agaactgtag tactgataaa tgtctttgct gctgttggtt tgtttactct tatctctatg
     9781 agatttggct ggtttatttg atgtagtgat tttgcaagca gcacgtactg tcagagaaat
     9841 caactctctg acagtacgca agatcttctc atactctcca ggtttacaac attatcctta
     9901 atatttactt ctttttgctg gcatctacat tgaaatgagt agaaaacatc cccctactgt
     9961 cgtctataca aacagcttac tgattcaaac ggaatcttgt tttcacattt ttctatcatg
    10021 aatactttat acgcgcggga gtctataaat gctttatact ttcaaggaat aacgt
//
LOCUS       DAZGJW010000164         9723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZGJW010000164 DAZGJW010000000
VERSION     DAZGJW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.164"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(69..281)
                     /gene="cspA"
                     /locus_tag="ACHKIK_004243"
     CDS             complement(69..281)
                     /gene="cspA"
                     /locus_tag="ACHKIK_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO9085302.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(562..852)
                     /locus_tag="ACHKIK_004244"
     CDS             complement(562..852)
                     /locus_tag="ACHKIK_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO9085303.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1286..1996
                     /locus_tag="ACHKIK_004245"
     CDS             1286..1996
                     /locus_tag="ACHKIK_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO9085304.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2046..3020)
                     /gene="ghrB"
                     /locus_tag="ACHKIK_004246"
     CDS             complement(2046..3020)
                     /gene="ghrB"
                     /locus_tag="ACHKIK_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO9085305.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3124..3783)
                     /locus_tag="ACHKIK_004247"
     CDS             complement(3124..3783)
                     /locus_tag="ACHKIK_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO9085306.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3936..6269
                     /gene="bisC"
                     /locus_tag="ACHKIK_004248"
     CDS             3936..6269
                     /gene="bisC"
                     /locus_tag="ACHKIK_004248"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO9085307.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSHGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6238..6678)
                     /locus_tag="ACHKIK_004249"
     CDS             complement(6238..6678)
                     /locus_tag="ACHKIK_004249"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO9085308.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6675..7238)
                     /gene="tag"
                     /locus_tag="ACHKIK_004250"
     CDS             complement(6675..7238)
                     /gene="tag"
                     /locus_tag="ACHKIK_004250"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO9085309.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7396..8094
                     /locus_tag="ACHKIK_004251"
     CDS             7396..8094
                     /locus_tag="ACHKIK_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO9085310.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8323..9525
                     /locus_tag="ACHKIK_004252"
     CDS             8323..9525
                     /locus_tag="ACHKIK_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9085311.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2314 a         2367 c         2517 g         2525 t
ORIGIN      
        1 ctgaaaaaca aatagaaaaa tccccgccaa atggcaggga tcttagattc tgtgctttta
       61 agcagagatt acaggctggt tacgttacca gctgccgggc ctttagcgcc gctttcgatg
      121 gtgaaggaca ctttctgacc ttcgtccaga gatttgtaac catcgttctg gatagcagag
      181 aagtgtacga acacgtcttt agagccatcg tcaggagtga tgaagccgaa gcctttgtca
      241 gcgttgaacc attttacgat accagtcatt ttaccggaca tagtgtatta cctttaataa
      301 ttaagtgtgc ctttcggcga tatggcgtgc tttacagatt ttgaagcgtt aaaggaatgt
      361 gcactacgag gggtatcaac gataactctt gaagggactt gccttactac actgctttaa
      421 tggtctgtac gtcaaaccgt tgatgtgcat taagccacgc attggcgggt gatgcaacaa
      481 ttatttttca tatttatgat taatcggttg gaaaacggtc ctgtcatcag gaccgtaaac
      541 agcaataaag tggataaaag tctattccat caactgctta cttaatgccg ggttggcttg
      601 aatcaaacgc atcaatttta gttcggcact tgaaggcttc acgcgtctgg attcccattc
      661 ctttaccatg gcgactgata cgcccaaaac ccgggcgaaa tcatcgattt ttaaccctgt
      721 cccttttcgt aactgctcaa tttcggtaca ggacgttgtt ctgtgcgtca gggtaatttt
      781 ctgcgtttca tctttaaaaa caatctgttc caggctgctc aacagctcat gcattggatc
      841 tttatattcc attgaaaact cctcaaatca cactgcgggg tcgtgaattg catcgaagct
      901 cattaagaat agtcgggaaa agcagacaca gcggggtgaa gtcagggcat gctctggcta
      961 tgattcgatt cagccagtca tttagcgtga cttatagcca cctaacgatc tgataaatgc
     1021 cgatatatct cttaccactc ctggtttgta aagatttcga aaatctaacc ttctgcgaag
     1081 gcgtaaatct ctggctacaa aaaaagcaac ggcactatca ttttttaaca atgaaacaac
     1141 aaattaacct tgtttcattt tctccatgat tttaccagga agattacgta gaaagggtat
     1201 cctgcaagcc tgtcctggac tatccttgtc acgtcagaca cgcgtgtgtc gttgtgcgct
     1261 ttttttgggt gaaaggagta agaaaatggc gacaggaaag tcctgctctc gctggtttgc
     1321 gcctcttgcg gcgttattaa tggtagttag cctgagtggg tgttttgata aagaaggcga
     1381 tcagcgtaaa gcgtttatcg acttcctgca gaatacagtg atgcgtagcg gtgaacgtct
     1441 accaaccctg actgccgatc agaaaaaaca gtttggtcct tttgtctctg attacgcgat
     1501 tctgtatggt tattctcagc aggtgaatca ggcgatggat tccggtctgc gcccggtcgt
     1561 agacagcgtt aacgcaattc gcgtaccgca ggattacgtt acgcaaagcg gtccgctgcg
     1621 tgaaatgaac ggttcgctgg gtgtgctggc gcaacaactg caaaatgcga agttgcaggc
     1681 cgatgctgcg cactctgcgc taaaacagac cgatgatttg aaaccggtct ttgatcaggc
     1741 attcaccaaa gtggtaacga cgccagctga tgcattgcaa ccgttgattc cggctgcaca
     1801 aactttcacg caacaactgg tgatggttgg agactacatt gctcagcaag gtactcaggt
     1861 gagctttgtc gcaaatggca tccagttccc gacttcacag caggcaagtg aatataacaa
     1921 actgattgcg ccattaccgg cacaacatca ggcgtttaat caggcctgga ctacagcagt
     1981 tactgcaacc caataaagag taaaagcccg agcgattatc gggctttact tcacgcagtc
     2041 gcggcttagt ccgcgacgtg cggattcaca cagttcttct caacctttcc ttgtaacgca
     2101 tcaatcaaat tatccacggc acaggcggcc atgccataac gcgtctcatg ggtggcagat
     2161 ccaatatgcg gtactgcgac gacgttggcc attgagagca acggcgaatc tacggacagt
     2221 ggctcttgtt cgaagacatc cagcccggcg gcgtgaattt cgcctttctg caatgcagca
     2281 atcagtgcat tttcatcaac caccgggcca cgtccggcat taatgaaaat ggcggaggat
     2341 ttcattttgg cgaattgttc tgcgccaaac agatgatgcg tctcatcagt taacggcagg
     2401 atcaggcaaa cgaaatctga ctcttgtaac agagtatcca aatcgcagta gcgggcgttg
     2461 aagcgttctt ctgcttcttt atggtggcgg cgcgcgttat agaggatggg catgttgaag
     2521 ccaaagtgcg cacgttgtgc cagcgccata ccgatccgtc ccatcccgac aatgcccagt
     2581 gttttatggt gaacgtcagt gccgtaccag tccgggccta tgctcgcggt ccattcgcct
     2641 gcttttaccc gttctgctac ctccacaacc cgacgagcgg tagacaacac cagcgccatc
     2701 agcgtatcgg cgacggtttc tgttaatacg gttggcgtgt gcatcagcag aatttttcgg
     2761 gcggtaagcg catcgacatc aaaattgtca tagccgacgg agatcgttga tgtggcacgc
     2821 agtttcggca ttttttccag caatgcggca tcaacattct cgtttgaacc cagtaaacct
     2881 tcagcttcgg caaaaattgc tgcattttgt tcgacggttt gtgggctgag gtttgccacc
     2941 tggtgaacgg tgaaatgctc ttgcaggcgt tgcagtaaat catcaggtaa ggctttgtag
     3001 aggataacgg acggcttcat gcttctctcc attcactgat aaggcgatca ccttagcctg
     3061 aaatatgatg aaggaaaact gaagaatgcc aggtgatttc tgcatcacct ggcatgaaag
     3121 ggattacagc gggcttaagg taatttctac acggcggttt tgcgccttac cttctgcggt
     3181 gctgttgctg gcgattgggt tagccgggcc aaggccctga gtacggatgc ggctggcgtc
     3241 cacgccctgg gtgatcaacg cgctggcaac ggaatccgca cgttgctggg agagacgcat
     3301 gttcaggtcg tgaccacccg tgctgtcggt ataaccaatc acgttaaccg ccgttttcgg
     3361 atactctttc agcaccattg ccacgccggt cagggtgtta gcgcccgcag gtttcagggt
     3421 cgcgctgctg ctgtcgaagg tcacattgtt cggcatattg aggataatgt tatccccgct
     3481 gcgggttacg ctaacaccag tgccgcgcat tttgtcacgc agcttcgctt cctgcacatc
     3541 catgtaataa cccacgccgc cgcccagagc tgcgcctgct gctgcgccaa tcagcgcgcc
     3601 tttaccgcga tctttcttcg aagaagagag cgcaccaata cccgcgccca cgagagagcc
     3661 cagacctgcg ccgatagcag atttacctgc ttcgcgttcg ccggtgtaag ggttagttgt
     3721 gcagccagat accgccagag cgccactcac tacggcggca ataagataaa cacgtttctt
     3781 cattgttaat ccttaataac ctttttattc tttgccacgg gttccgtggc gggagattat
     3841 gccgcgtgaa catgaagatt attcctggga atactcggaa atttgtaagt aatatttaac
     3901 tgctcaatac atctaacctt tcaggagttt tcggtttggc caactcatcc tcacgatatt
     3961 ccgttctgac tgccgcccat tgggggccca tgctggttga aaccgacggc gaaaccgtgt
     4021 ttagctcgca tggcgcgtta gccacaggaa tggaaaactc cttgcagagc gcggttcgcg
     4081 accaggttca cagcaataca cgggtacgat ttccaatggt gcgcaaaggc tttcttgcgt
     4141 caccggaaaa tccgcaaggc attcgtgggc aggatgaatt tgttcgcgtg agttgggatg
     4201 aggcgctgga tcttattcac caacaacata aacgcattcg tgaggcttat ggtccggcat
     4261 cgatttttgc tggttcctac ggctggcgtt caaacggcgt gctgcataag gcctcgacat
     4321 tattacaacg ctatatggcg ctggcaggcg gttataccgg gcatctgggg gattattcga
     4381 ccggcgcggc acaggcgatc atgccgtatg tcgtgggtgg tagcgaagtt tatcaacagc
     4441 aaaccagttg gccgctggtg ctggaacata gcgatgtcgt ggtgttgtgg agtgctaacc
     4501 cactcaatac gctgaaaatt gcgtggaatg catctgatga gcaggggctt tcttactttt
     4561 ccgcactgcg tgacagcggg aaaaagctga tctgtattga tccaatgcga tcggaaaccg
     4621 tcgatttctt tggcgataaa atggagtggg tggcaccgca catgggcacc gatgttgcgc
     4681 tgatgctggg gatcgcccat acgctggtgg aaaatggttg gcacgacgaa gcgtttctga
     4741 cgcgttgcac cacaggttat gccgtcttcg cctcttattt gctgggcgag agtgacggaa
     4801 tagcgaaaaa cgccgaatgg gcggcagaga tttgtggggt taacgcagcg aaaatccgcg
     4861 agctggcggc tcttttccac caaaacacga ccatgctgat ggctggctgg ggaatgcagc
     4921 gccaacagtt tggtgagcaa aaacactgga tgatcgtcac gctggcggca atgttggggc
     4981 aaatcggcac acccggcggc ggttttggtc tttcttatca ttttgccaat ggtggtaacc
     5041 ccacgcggcg ttctgcggtg ctctcttcca tgcagggcag cttgccgggt ggctgcgatg
     5101 cggtggataa aatccctgtt gcccgcattg ttgaagcact ggaaaaccct ggtggcgcat
     5161 atcaacacaa cggtatgaac cgacatttcc cggatattcg ttttatctgg tgggcgggcg
     5221 gtgccaactt tactcatcat caggatacca atcgtctgat ccgtgcctgg caaaaaccgg
     5281 agctggtggt gatctctgaa tgcttctgga cggcggcggc gaaacacgcg gatatcgttc
     5341 tgcctgcgac tacctctttt gagcgtaatg atctcaccat gaccggtgat tacagtaatc
     5401 agcatctggt gccgatgaag caagtggtgc cgccacgcta tgaagcgcgt aatgattttg
     5461 atgtttttgc cgagttgagt gaacgttggg agaagggcgg ctatgcgcgg tttacggaag
     5521 gaaaaagtca gctgcaatgg ctggaaacgt tttataacgt tgcccggcag cgcggggcaa
     5581 gccagcaggt tgaattgccg ccatttgctg agttctggca agccaaccag ttaattgaga
     5641 tgccggaaaa cccggacagc gagcggttta ttcgcttcgc tgatttttgc cgcgatccgc
     5701 tggcgcatcc gttaaaaacc gccagcggca agattgaaat cttctcacag cgtattgccg
     5761 attacggtta cccggattgc cctgggcatc caatgtggct ggagccggac gaatggcagg
     5821 gcaatgccga accagaacag ttgcaggtac tttctgccca tccggcgcac cgcctgcaca
     5881 gccagctgaa ttacagttct ctgcgcgaat tgtacgcggt agcaaaccgt gagcctgtca
     5941 ccattcatcc tgacgatgct caggcgcgcg gcataaccga aggggatatg gtgcgggtgt
     6001 ggaactcccg tgggcagatc cttgccggag cggtcattag cgagggaatt aaacctggcg
     6061 tgatttgcat tcacgaaggg gcatggccgg atctggattt aaccgctgac ggtatttgta
     6121 aaaacggcgc agtgaacgtg ctgaccaaag atctccccag ctcgcggctg gggaatggct
     6181 gtgcgggtaa tacggcgctg gcatggctgg aaaaatacaa cggtccggaa ctgacactta
     6241 cagcgtttga accaccggcc agctcataat ccaggtgggt agttgggttt catcctgcca
     6301 tgcgcaatcg acaatgtgaa aaccctgcgc acggtaaaaa tctatcgccg gttgattttt
     6361 ttgataaacc tccagcatca gatggggata gcgctgctgc acatactgca tcagcgcctt
     6421 accaataccg cgtctgacgg ccttcggtgc gacaaacatt gctgccagaa atcggccttc
     6481 cataatgctg acaaaaccga gaagcttacc gtcctcttcc cagacccagt tttgcgcgtt
     6541 ggcaagatag gcatcccgca ccagcggaat gcagtcacgc cagtaattcg cttttataaa
     6601 gggatgcccc caggttgtac tttccagcca cagttcgagg atcgcgggga gttctgaacg
     6661 ttgcgcttcc cgaatcatgg tttatttccc ggatagcaac agcagccaac cacatgatca
     6721 ttcaccagcc cacatgcctg cataaaggag taacagattg tggtgccgac aaacttaaaa
     6781 ccacgttttt tcagtgcctt agatagggcg tcggaggcgg acgtagatgt gggaatttcg
     6841 ctcaacgttg tggcttgtgt cacctgtggc tgatgattta caaacgacca gacaaagtcg
     6901 gcaaacggtt cgccgttctg ttccatttgc aggtacgccc gcgcattacc aataattgcc
     6961 tgaattttcc ctcgatggcg gataatcccg gcgtcctgta ccagtctttc gacatcctct
     7021 tcctgcattg ctgcgacctt caccggatcg aactgatgaa agcaggcgcg atagttttcg
     7081 cgttttttga ggacggtgat ccacgataac ccagcctgct gcccttcaag gcagatcatt
     7141 tcgaacagtt ttttactgtc agtttcaggc acgccccatt cattatcatg gtaggcaata
     7201 taaagcgggt cctgactcac ccagccgcaa cgttccatac tttccctcgc tatgtacccg
     7261 atcgtaaaaa tttcagccgc ttttcttgac gcatcggtga aaaagtcaat atttaataca
     7321 gggctactat gcccgatatc cttcatgcaa tgacaataat attgccgaat tcggctcttc
     7381 cctggagtcg tgttaatgat cataaaaaaa agcggtggtc gctggcagct aagcctgctg
     7441 gcgagcgtgg taatcagtgc cttttttctc aacacagctt acgcctggca acaagaatat
     7501 atcgttgata cgcaacccgg acattccaca gagcgttaca cctgggatag tgatcatcaa
     7561 cctgattaca acgatatttt gtcgcaacgt attcaaagta gccaaagggc gctgggactg
     7621 gaagtcaatc tggcggaaga aactcctgtg gatgtgacca gcagtatgag tatgggctgg
     7681 aattttcctt tatatgaaca ggttacaacc ggcccggtcg cggcattaca ttacgatggc
     7741 acaaccacct cgatgtataa cgagtttggc gacagtacta ccacgctgac cgatccgtta
     7801 tggcatgcca gcgtgagtag cttaggctgg cgtgttgact cccggcttgg cgatctccga
     7861 ccctgggcgc aaatcagcta taaccagcaa tttggcgaga atatctggaa ggcgcaatca
     7921 ggcctgagcc ggatgacggc gacaaaccag aacggcaact ggctggatgt caccgtaggc
     7981 gctgatatgt tgctcaatca aaatattgcc gcctatgccg cgctaactca ggcagaaaat
     8041 accactaata atagcgacta tctgtatacg atgggggtta gcgccagatt ttaacgtaac
     8101 agtcacaatt gaaaccattg aataacaaca gttgtggcga tagtgggtgc taacttacca
     8161 aataataaat ttggtgaata attgtcgcgt cattcattcc tgaactaagg catttcattc
     8221 cgttctgatg gcatttcatg ccgtttttcc ccaggcataa aatgcacttc gttatggttg
     8281 tcggcagaga tttttccttt ttattactgc aggaatactg ccatgacacc ttcaaattat
     8341 cagcgtaccc gctggctgac actcatcggt actatcatta cccagtttgc actggggtcg
     8401 gtttatacct ggagcctgtt taatggcgcg ctttctgcca agctggatgc gccggtaagc
     8461 caggtcgctt tctctttcgg cttgttaagt ctggggctgg caatttcgtc ttctgttgcg
     8521 ggcaaattgc aggaacgttt tggcgttaaa cgcgtcacca tggcttccgg cattttgctg
     8581 ggattaggct tcttcctgac cgcgcattcc aacaacctga tgatgctgtg gttaagcgcc
     8641 ggtgtgctgg tcggtctggc ggatggcgcg ggttacctgc tgacgctctc taactgcgtg
     8701 aagtggttcc cggagcgtaa aggtctgata tccgcgttcg ctatcggttc ttatggtctg
     8761 ggtagcctgg gtttcaaatt tatcgacacg cagctgctgg aaacggtggg tctggaaaaa
     8821 acctttgtga tttggggagc gattgcgctg gtgatgattg ttttcggcgc aacgttaatg
     8881 aaagacgcac caaaacagga agtgaaaacc agcaatggtg tggtggagaa agattacacg
     8941 ctggcagagt cgatgcgtaa accgcagtac tggatgttag cggtaatgtt cctgaccgcc
     9001 tgcatgagcg gcctgtacgt gattggggta gcgaaagata tcgcccaaag tctggcacac
     9061 cttgatgtgg tttccgcagc caatgcagtc actgttattt ccatcgccaa cctttcaggt
     9121 cgtctggtgc tgggtattct gtctgacaaa atcgcccgta tccgtgttat caccattggt
     9181 caggtgatat cgctggtggg tatggcggcc ctgctgtttg caccattgaa tgcagtgacg
     9241 ttctttgcag cgattgcctg cgtggcattt aactttggcg gcactattac cgtctttccg
     9301 tcactggtca gtgagttctt tggcctcaat aacctggcga aaaactacgg tgtgatttac
     9361 ctcggtttcg gtatcggtag catttgtggt tcgattatcg cctcactgtt tggcggcttc
     9421 tatgtgacct tctacgtgat tttcgccctg ctgattctgt cattggcgct ttctacgaca
     9481 attcgtcagc cagagcagaa agtattgcgt gaggcgcata tttagaaata tgttgctact
     9541 gaaataaatg cgaggaggga gacttcaaag tctccctttt tttatggacg catattaaaa
     9601 gaacaataat atttacacca caaatatatt ttatttgttt atatatcatg taaatataaa
     9661 tatattccta aaaaaatagt cgttggaagc gtttttgtgt gcaattaatt gaagttgaag
     9721 tac
//
LOCUS       DAZGJW010000165         9700 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZGJW010000165 DAZGJW010000000
VERSION     DAZGJW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.165"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1181
                     /locus_tag="ACHKIK_004253"
     CDS             <1..1181
                     /locus_tag="ACHKIK_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9085312.1"
                     /translation="DNTGAEPADEAGSEQPEETAETVSPVQRLAQLTAELDALHNDRL
                     LVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWLGDTIKGQDLAIASLHKH
                     LLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMSEFQEKH
                     TVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAFDKGEM
                     ADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPALLARMEV
                     VPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVIIEPEVTDEIMSRVTRAENGARML
                     ESVIDGNMLPPLSLLLLQKMAANTAVARIRLSAVDGAFTADVEDAQNDESVTKDETVL
                     "
     gene            1178..1921
                     /gene="vasI"
                     /locus_tag="ACHKIK_004254"
     CDS             1178..1921
                     /gene="vasI"
                     /locus_tag="ACHKIK_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO9085313.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            1926..3338
                     /gene="tssA"
                     /locus_tag="ACHKIK_004255"
                     /pseudo
     CDS             1926..3338
                     /gene="tssA"
                     /locus_tag="ACHKIK_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
     gene            3447..6881
                     /gene="tssM"
                     /locus_tag="ACHKIK_004256"
     CDS             3447..6881
                     /gene="tssM"
                     /locus_tag="ACHKIK_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO9085314.1"
                     /translation="MLILAWIFLLVWIWWKGPMWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFITRYGLQSYFVKQRDELVELTAMDSWVLNLTRNVKYSDADRAEIQHQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            6892..8341
                     /locus_tag="ACHKIK_004257"
                     /pseudo
     CDS             6892..8341
                     /locus_tag="ACHKIK_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            8268..8750
                     /locus_tag="ACHKIK_004258"
     CDS             8268..8750
                     /locus_tag="ACHKIK_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO9085315.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            8794..>9700
                     /locus_tag="ACHKIK_004259"
     CDS             8794..>9700
                     /locus_tag="ACHKIK_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085316.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNAGKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIISSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPP"
BASE COUNT         2357 a         2605 c         2698 g         2040 t
ORIGIN      
        1 aggataatac cggtgccgaa ccggctgatg aagctggcag cgaacagccg gaagagaccg
       61 ctgaaacagt ttccccggta cagcggctgg cccagctcac tgccgaactg gacgccctgc
      121 ataacgaccg gttgctggtc tccccgcacg tcgataaaaa acagattgcg gcggtgattg
      181 ccgaatggac cggcgtgccg ctcaaccgcc tatcgcagaa tgaaatgtcg gtcatcaccg
      241 acctgacgaa atggctgggt gacaccatca aaggccagga cctggcgatt gccagcctgc
      301 ataagcatct actgaccgca cgcgccgacc tgcgtcgtcc gggacgcccg ctcggtgcgt
      361 tcctgctggc tggccccagc ggtgtgggta aaaccgaaac cgtcctgcaa ctggcagagc
      421 tgctctacgg cggtcgccag tacctgacca ccatcaatat gtccgaattc caggagaaac
      481 acaccgtctc gcggctgatt ggttcgcctc cgggctacgt tggctacggt gaaggcggcg
      541 tactgaccga agcgattcgc cagaaaccct actcggtagt actgctcgat gaagtggaaa
      601 aagcgcaccc ggatgtgctc aacctgttct accaggcgtt cgataagggc gaaatggcag
      661 acggtgaagg ccgcctgatt gactgcaaaa atatcgtctt cttcctgacg tccaacctcg
      721 gctaccaggt aatagtcgag catgccgatg acccggaaac catgcaggaa gtactgtatc
      781 cggtgctggc cgacttcttc aaacctgccc tgctggcgcg tatggaagtg gtgccgtacc
      841 tgccgctgtc gaaagagacg ctcgccacca tcattgccgg gaaactggcc cgcctggata
      901 acgtgctgcg cagccgcttt ggcgcggagg tgattataga accggaagtg acggacgaaa
      961 tcatgagccg cgtcacccgc gcggaaaacg gcgcgaggat gctggaatcg gtcatcgacg
     1021 gcaatatgct gccgccgctc tccctgctgc tgttgcagaa aatggcggcg aatacggcgg
     1081 ttgcccggat tcggttgtcg gcagtggacg gcgcatttac ggcagacgtg gaagatgctc
     1141 agaacgacga gtccgtcaca aaggatgaaa cggttttatg agcaggttgc gcgttttccg
     1201 gcagacagga tggtttatcg cgggcctgat gacgggctta cccgccactg ccgcgcccgc
     1261 agaagcatcg tcaatggccg gtgtggcggt cgccgtcgcc accacgaccc cgccggatgc
     1321 gacagccacg ttacaggcca tgcagtcctg tcggcgggag tccgcggcgc tggagcggct
     1381 tgactgctat gaccgcctgc tggcaccgct gtccccgtca gggtttgacg gggcgctggt
     1441 caaagccggt tttgtgggcg aggcctggac ccgcgccaca gagcaggaaa aacgccgcga
     1501 gggcaacacc acagaactgc tggtgacgca ggtgccgggg gagcgcccga cggtggtgat
     1561 aaccaccccg gctatcggtc atgttccgcc acgcccggtg ctgatgttca gttgcgtgga
     1621 caatatcacc cggatgcagg tggcgctgat gcaccctctg gatgtccatg atatcgccgt
     1681 cactctgaat gccgacaacc gggcattgcg cagccactgg tttgtgcgcg agaacggcac
     1741 cctgctggag tccagccggg ggttatccgg gattgatgaa attaagcaac tgttcggcgc
     1801 aaaaacgctg acggtggata ccggcgcaga caatgccgcc ggaaagctga cttttaacat
     1861 tgacgggctg gcaagggcga ttgctcccct gcgcgatgcc tgccactggg cgggagaata
     1921 aaaatatggc aatggactta cgcgatccga atgtctggat atcgcacctg ctggaaaacc
     1981 tgccggaaga gaaactggca tcggcgctga aagatgacaa cccgaactgg gagtatatcg
     2041 acggcgaaat cgtcaagctg ggatcgctgg cccacagtca actggatatc ccggaactcc
     2101 agcgccgtgg gctggtcatt ctggcttcgg agagtaagga ttttcgtctg ctggcgcatc
     2161 tgctgagaac cctgcaacac gccggtgatc ctcatctcgc attgcgcctg ctggcgctgt
     2221 atgtggagca ttactgggcg gtggctgcgc cgcagaatat ggcgcataaa aaacgctttg
     2281 ccagccaggt cattaaacgc tttgaaacag gtattgaagt tttttcacag aacgccgcca
     2341 cagcgcagcg cgatgcgctg tcaggggaac tggcaaaact ggcgcaatgc tggcagtcac
     2401 ataacgcccc ggaactggca caggctactg atgatctttt tgccctgtac cagcgtgcgt
     2461 ttaatcgtgc ggcaccggct cctgtcccca ctccggcggc ctccggtagt tcaccacaga
     2521 ccaccgccac gtctgaaagc ggcgtgacgc aacccagtgc tccggctccc caaatagtca
     2581 tcgacagtca cgacgacaaa gcctagcgcg acacgctgtt aaaagtggcg gccattttat
     2641 gtgaacgcca gccggattca ccgcagggct accgtctgcg ccgccatgcg ctttggcaga
     2701 acatcaccag tacgccccag gcggaaagcg atggccgtac tccactggct gcggtctccg
     2761 ccgatatggt ggcagattac catgctcagc tcggtagtgc cgatatggcg ctgtggcagc
     2821 aggtggagaa aagcgtactg ctggctcctt actggctgga tggtcactgc ctttctgcac
     2881 agacggcgct gcgtctgggt tacaaacagg tggcagacgc catccgcgat gaggtcatcc
     2941 gcttccttga gcgtctgccc cagcttaccg ggctgctgtt taatgaccac acaccgttta
     3001 tcagtgagca gacgaaacaa tggctggcag cttcgcccga cgccaaagtt gcaccggttg
     3061 cgcaaatcgg tgaggaatcg aaggcagcca gagcctgttt tgctgagcag ggtctggagg
     3121 cggcgctgcg atatctggac atgctacccg aaggcgatcc ccgcgatcag tttcaccgcc
     3181 agtaccttgc cgcacagttg acggaagagg cggggctggt acagcttgcg cagcagcagt
     3241 accggatgtt gttcaggatg ggattgcaga tgatggtggc agactgggag ccgtccctgc
     3301 ttgagcagct agaacaaaaa ttcacggcag aacaataaaa gagaacagga gtttttgtgt
     3361 tcagattacc aacaccccga ctactcagcg gactcaaatc agccctgcga ccggcgatgc
     3421 cccggtttaa agtctctgct ttctggctgc tgatactggc gtggattttt ctgctggtgt
     3481 ggatctggtg gaaaggcccg atgtggacgc tgtatgaaga acagtggctc aaaccactgg
     3541 cgaaccgctg gctggcaacg gcggcgtggg ggattattgc cctggtgtgg cttaccgtcc
     3601 gggtgatgaa gcgcctgcaa cagcttgaaa aaatgcaaaa gcaacagcgc gaggaagccg
     3661 ttgatccgct cagcgtggaa ctgaacgccc agcagcgtta tcttgaccgc tggctgctgc
     3721 gcctgcaacg ccatctcgac aaccgccgtt tcctgtggca gttgccgtgg tatatggtca
     3781 tcggcccggc gggcagtggc aaaacgacac tgctgcgcga ggggtttccg tccgacatta
     3841 tttatgcccc ggagggcgca cgcggcgcag aacaacgcct gtacctcacg ccccatgtcg
     3901 gtaaacaggc ggtgatcttt gatatcgacg gcacactctg cgctcccgct gatgcggata
     3961 tcctgcatcg ccgtctgtgg gaacatgccc tcggctggct gaaggaaaag cgcgcacgcc
     4021 agccgctgaa cgggattatt ctgacactcg atttacccga tctgctgacc gcagacaaac
     4081 gccgccgcga gcatctgtta cagacgctgc gcagccgctt acaggatata cgccagcatc
     4141 ttcactgcca gttaccggtt tacgtggtac tgacccggct tgatttatta cagggttttg
     4201 ccgccctgtt ccagtcactc aacagacagg atcgcgatgc gattctgggc gtcacgttca
     4261 cccgccgcgc ccatgaaaat gatgactggc gaacagagtt gaatgctttc tggcagacat
     4321 gggtggatcg aatgaatctg gcgttgccgg atctgatggt cgctcagact cacacccgcg
     4381 cgtctttatt cagtttttcc cgccagatgc agggaagccg tgaaccgctg gtgtcactgc
     4441 ttgagggtct gcttgatggc gaaaatatga acgtgatgct gcgtggtgtc tatctcacct
     4501 cttcgcttca gcgtgggcag atggatgata tattcaccca gtctgccgcc cgccagtacc
     4561 ggctgggcaa taacccactg gcgtcctggc ccctggtgga caccgcgcct tatttcaccc
     4621 gcagcctgtt cccgcaggca ttactcgcag agcctaatct ggcaacagag agccgcgcct
     4681 ggctgatacg ttcccgtcgc cgcctgacgg ttttctctgc cacaggcggc gtggcagcac
     4741 tgctgctcat caccggctgg catcactatt acaacggtaa ctatcagtcc ggcatcaccg
     4801 tgcttaagca ggccaaagcc tttatggacg tgccgcctcc gcagggggaa gatgactttg
     4861 gcaacctgca actgccgctc ctgaacccgg tacgcgatgc cacactggcc tatggcgact
     4921 ggggcgaccg cagccgtctg gccgatatgg gactgtacca gggacgacgt atcgggcctt
     4981 atgtggaaca gacctatctg caactgctgg agcaacgtta cctgccctcg ctgtttaacg
     5041 ggctggtcaa agcgatgaac gccgcgccgc cggagagtga agaaaaactc gcggtgctgc
     5101 gcgtaatgcg aatgctggag gacaaaagcg gacgtaacaa tgaggtggtg aagcagtata
     5161 tggcaaaacg ctggagcgaa aaattccacg gccagcgcga tatccaggca caactgatgt
     5221 cccatcttga ctacgcgctg gctcatactg actggcatgc agagcgtcag gcgggcgacg
     5281 gtgacgccat cagccgctgg acgccatatg acaagcccgt ggtatcagca cagaaagaac
     5341 tgagcaaact gcctgtctac cagcgggttt accagagcct gaaaacgcgg gcgctgggcg
     5401 ttcttcctgc cgacctcaat ctgcgtgacc aggtagggcc aacctttgac caggtgttta
     5461 catctgccga tgacaacaaa ctggttgttc cacagtttat cacccgctac ggcctgcaaa
     5521 gctattttgt aaaacagcgc gatgaactgg ttgaactgac agcgatggat tcctgggtac
     5581 ttaacctcac ccgcaatgtg aaatacagtg acgccgaccg tgcggaaatc cagcaccagt
     5641 tgaccgagca gtatatcagc gactacaccg ccacctggcg ggctgggatg gacaatctga
     5701 atatccgcaa ttttgagtcc atcggacaac tgaccggggc gctggagcag gttatcagcg
     5761 gcgaccagcc tttgcagcgg gcgctgaccg tgctgcgtga caacacacag ccaggcgtct
     5821 tttctgaaaa actctctgcc aaagaacggg aggaagccct ggcagagccg gattaccagt
     5881 tactcacccg cctcgggcat gaattcgccc cggaaaacag taccctggca gtacagaaag
     5941 acaaagaaag cacgatgcag gccgtgtatc agcaactcac cgagttgcac cgctacctgc
     6001 tggcaatcca gaacgcgcct gtaccaggga aatcggcgct gaaagccgtg cagttacggc
     6061 ttgatcagaa cagcagcgat ccgatattcg ccacccgcca gatggcaaaa acgctgcctg
     6121 ctccgctcaa ccgctgggtt ggcaggctgg ctgaccaggc ctggcatgtg gtgatggtgg
     6181 aggctgttca ttatatggaa gtggactggc gcgacagcgt ggtgaaaccg tttaacgagc
     6241 aactggcaaa taactatccg tttaatccgc gttctgcaca ggatgcctca ctggatgcct
     6301 tcgaacgctt ctttaaaccg gatggcatac tggatacctt ctaccagcag aacctgaagc
     6361 tgtttatcga taatgacctg agtctggagg atggcgataa caacgtcatt attcgcgaag
     6421 atattattgc gcaactggaa actgcgcaga aaatccgtga catcttcttc agcaaacaga
     6481 acggtctggg aacatccttt gccgtggaaa cggtatcgct ttcaggcaat aaacgccgca
     6541 gtgtactgaa ccttgacggt cagttagtcg attacagcca gggccgtaac tataccgccc
     6601 atctggtctg gcctaacaac atgcgcgaag gcaacgaaag taagctgacg ctcatcggca
     6661 ccagcggcaa cgcgccgcgc agtatcagct tcagcgggcc gtgggcgcag ttccgcctgt
     6721 tcggggccgg acaactgacc ggagtacagg atggcaactt taccgtgcgc tttagcgtgg
     6781 acggtggcgc gatgacctac cgtgtgcata ccgacacgga agataacccg ttcagcggtg
     6841 ggttgttcag ccagtttggt ctgtcagaca cactgtactg aggaggcagg gatgaatagt
     6901 aacgtactga cacaaactat cgttaccggc agtgacccgc gcgggctgcc ggaattcagc
     6961 gccatccgcg aggaaataaa caaagccagc cacccgtcac agcctgagct gaactggaaa
     7021 ctggtggagt cgctggcgct ggcgattttt aaagccaacg gtgtggattt acacaccgcc
     7081 acctactata cgcttgcccg gacacggaca cagggactgg cgggattctg cgaaggtgcg
     7141 gaactgctgg cggcaatggt aagccacgac tgggataagt tctggccgca gggcggcccg
     7201 gcgcgtactg aaatgctgga ctggtttaac tcccgaaccg gcaatattct gcgtcagcaa
     7261 atctcctttg cggaatccga cctaccactg atctaccgca cagagcgggc attgcagctt
     7321 atctgcgata agctccagca ggtggaactg aagcgcgttc cgcgcgtgga gaatctgctc
     7381 tattttatgc agaacacgcg taaacggctt ggaccccagc tgaagagtaa cactgagaac
     7441 gccgcacaga ccacggtcag aacgctgatt tatgccccgg aaacacaggc atcttccaca
     7501 ccagaagcgg tagtgcctcc cctgcccggc ctgcctgaga tgaaagtgga agttcgcagt
     7561 ctgacagaga atcccccaca ggccagtgtg ataaatcaag gcagtacggt aagagggttt
     7621 atcgcaggga tcgcctgttc agtggctgtc gcctcagcat tgtggtggtg gcaggtctat
     7681 ccggtgcagc agcaactgtt acaggttaac gacaccgctc agggcgcagc aacggtgtgg
     7741 atggcctcac ctgaactcga aaactatgag cgcaggctgc aacaacttct tgatacctcc
     7801 ccggtacagc cgctggaaac cgggatgcag atgatgcgtg ttgccgacag tcgctggccg
     7861 gaaagcctgc aacagcaaca ggcctcgaca caatggaatg aggcactcaa aacccgcgca
     7921 cagagtagcc cgcagttgcg tggctggttg cagacccgcc aggacttaca tgcttttgca
     7981 gatctagtga tgcagcgcga gaaagaggga ctaacccttt cctatatcaa aaatgtcatc
     8041 tggcaggcgg agcggggact ggggcaggaa acacccgttg agtctctgtt gacgcagtac
     8101 catgatgccc gtgcgcagaa gcagaataca gatgcgctgg aaaaacaaat taatgagcga
     8161 ctcgaaggcg tgttaagccg ctggctgctg ctgaagaata acgtcatgcc agaggcggca
     8221 accggtacca cggccgaaaa ataattattt aattaaagga gattatcatg gcaaatataa
     8281 gttatttatc tttgagtgga gagacacaag ggctaatttc agcaggttgc tcaacgcttg
     8341 attctgtcgg aaacaaagcg cagccagaac ataaagatca gatcatggtt tatgcgttaa
     8401 tgcattccat ctcaagaagt caaaatgtca atcatcatga attaattatc accaaaccgg
     8461 ttgataaatc ctcaccattg ctggcaaaag cacttagtga caatgaaaaa atggcaatat
     8521 gtgaatttat actctaccga accagtaaag cgggtattta ccagccttat tataaaataa
     8581 atctgtcaaa agcccgaata tcaagtattg attttgtcac accgcatgct gtcctggaaa
     8641 aagaactgga accacaggag agaattgcct ttatttatga agatatttcc tgggagcata
     8701 cgttagccgg tacgaatgca atgagtaaat ggcaggatcg tgtacagtaa cttactgtaa
     8761 taacataata aattactatt gcggagagat aaaatggctg gtttaaggaa taacaacaac
     8821 acacagaatg cacagtgggc tgattatgtg ggtgacattc ttcggggggc tcagccgata
     8881 aaccagctag tacctcagca cccttatctt aacgacgtcc cactcattga tgagttaagg
     8941 catcagaaca ctcatcatgt tattctcttg acacttgatg ttgcaaaaaa aatactcagc
     9001 ccaataacat catttgatta cattcatttt atcactacac atccttcagg tataaaagat
     9061 acactcgcct ggttagttaa cgcgggtaaa ttaatgactg agtttgatga caatgggaaa
     9121 attatattta accttaatgc cttaaagtat acaaaagcaa gttactttga aatacttgga
     9181 gagaaatata taaaaatcac aacatccagc ccctggcttt tagaaaagct cggtaaatat
     9241 attttctctt cacgagcgcc tcaagttctg gaactcgcta ttggctggcg aggagcttta
     9301 tctgaatcaa taaaaggagt taagttctgt atctggtttt cagtagcctg gagaaccata
     9361 gaatttatta taagttcaga gcgagatctg gttaattttc ttggcgattt ttcaatggat
     9421 gtggccaaag cagttatagc cggtggtgtt gcaaccgcta ttggaagcct ggcttctttt
     9481 gcctgtgtta gctttggctt tccagtaatt cttgtcggag gagcaatttt actgacaggg
     9541 atagtgtgta cagttgtttt aaatgaaatc gatgctcaat gccatttatc agaaaaatta
     9601 aaatatgcaa ttagagatgg actaaaacgg caacaggaac ttgataaatg gaaaagggaa
     9661 aacatgactc catttatgta tgttcttaac actccacccg
//
LOCUS       DAZGJW010000166         9688 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZGJW010000166 DAZGJW010000000
VERSION     DAZGJW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.166"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            835..3024
                     /gene="zorA1"
                     /locus_tag="ACHKIK_004260"
     CDS             835..3024
                     /gene="zorA1"
                     /locus_tag="ACHKIK_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I Zorya anti-phage system protein ZorA1"
                     /protein_id="HFO9085317.1"
                     /translation="MSWLNSILVTLTSVEPYKVPVTVIVTVTFAFVCFIFFYLLRSIR
                     IIYGLKKYTRSINSIEKSAPEVQLEHLKSLFQRSELKHAWNEFEESLHSQYELENGEE
                     KIVRIRATAPSASFFSEQQLVDIPLNTEFFKHLPGILTGMGIIGTFYGLMIGLNHFDP
                     STPEQVSSSVNNLLRDVLYAFLGSAFAIFASILVTWLEKLSIAKSYKYLEKFTAALDS
                     LYDSGVGEEYLASLVKSSNESATQARHLKESLVTDLRDMLLHLAESQKIENERLANTL
                     SATYRESGSQFADQVSGAIENSLKSPLDKIAGAVQTASGDQSGMVQNMLQNVLTAFMA
                     KLDTTFGQQFTNLNEMMGQTVGAIQTMQTGFSALLQDMRQVSDDSRQGSAQLIEQLLS
                     EMKSGQQALQAGMNDMLTSLQVSVAKIGAEGEGAGERIARQLEKMFADSEAREKAQAE
                     HMAAFVEAIQNSVQQGQSATMEKMAASVGALGEQLGSLFGQIDKGQQQISATQQANQQ
                     SLHEQTQRVMSEVDDQIKQLVETVASQHQGTTETLRLLAEQTNRQIQDMQAGADKMRL
                     AAERFEHAGERVSEANHLTADVLNKAQSAGSSLSLATSELTSVVADYRNNREAVSKSI
                     AMLELLAANTQSEQTTRNQFIADLKQHGERLQSYNREAQVFMENVSDVLGKGFEDFSE
                     GVSRSLDKTLGKLDVEMAKASNLLAGSVEQLGESVSELDDVLSRVRT"
     gene            3038..3778
                     /gene="zorB1"
                     /locus_tag="ACHKIK_004261"
     CDS             3038..3778
                     /gene="zorB1"
                     /locus_tag="ACHKIK_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I Zorya anti-phage system protein ZorB1"
                     /protein_id="HFO9085318.1"
                     /translation="MFGNAFGVKKRRSDEAEKPFWISYADLMTAMMVLFLVVMVASLS
                     SVTQRIQRAEQGEKARGQDISRLCERLELHARNVNKNIVVDCHDNRISFGEAGRFAHN
                     QFFLNAEGQKALQDVVPLVLEASNSEEGKKWFKQIVIEGFTDTDGSYLYNLHLSLQRS
                     EWVMCSLLDSRSPLQKNISAEQQLQIRKLFLAGGVSFNNAKESKEASRRVELRMQFFG
                     LKDKRDKADEVDFPPVVNKEVCQLVMPL"
     gene            3775..5457
                     /gene="zorC"
                     /locus_tag="ACHKIK_004262"
     CDS             3775..5457
                     /gene="zorC"
                     /locus_tag="ACHKIK_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I Zorya anti-phage system protein ZorC"
                     /protein_id="HFO9085319.1"
                     /translation="MIPALNSLSQRIAARLSSSQRDDHYLHNDFHALTAATLDMEKRF
                     DRAERIPSPPQEMRLEALRRFRLAQELTEREWRMVFYGLADNDPLYPDQPILLEDDTF
                     FPRVNSAIKKRIETKTLKRRDWAALCSSYFAYQHQSPETNPHWCVLRGHIAQGYLVVK
                     AAIRREKSWMKTIEFYHDIFTPQAGGVISRQLLAGENNSLSSLEKIAQIPDSSWLWKR
                     IFTVLLAQLDTLDDPQFLSKISWLLGLAAQWVRFRDDIMTATLTRYYHSGYRDQSHPA
                     LKQAALEYWDNPQLKSQQNKWHQYVSESVAAMVRGWLAKQDLTHFFELLRGNGDVDQA
                     RLHYWLRFANQMGFTRIIMGSDAWQDRGSDFVKFREENKGRLSYLRGGRNFDNAMVMQ
                     INDYLFVEFSGTGNAMYAYQIGHAPFNPESRTLDINIHLKDQGRCALRLPHAPRAEGY
                     NKVRITGWMLKYDDELRKLGIRWMAEEPVRFVDKKVPPPVAMSDIKIINPLRDTAIQR
                     LVKCSSCIVSDNRHKGGILSVQLITPDDTVERELLRLGFAPVAKEPHRYWIK"
     gene            5457..8699
                     /gene="zorD"
                     /locus_tag="ACHKIK_004263"
     CDS             5457..8699
                     /gene="zorD"
                     /locus_tag="ACHKIK_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I Zorya anti-phage system protein ZorD"
                     /protein_id="HFO9085320.1"
                     /translation="MLKRLLSKLTGNRQQIEHHLKNQYQVEENGLSFPLSLVDDSQLW
                     ALASWLEQLAEEDYLISLTDRWLLSWEALYRLLEDEEHASSLPLIGVPDILPLRASLS
                     SRGALSDSDFRVWIAEWATFPARKPIRFSRTGAILTHDNQQYLLSRENWALLQATEQL
                     SAQQIQTPGETTNQLGWAAIRKCAKLAAAKFDDYLEKTHVIKPTSLSLRLRKATVADT
                     AVIEIEPHFEDQPANWLGSFDKNLQVHDSYRIPGENGELSHVIIPPEVKEVLNSIHSI
                     PGRRVAGSEALSFVRNPYTFLGEDAASVIAPEEHEQALFDARIFFHHFRLIPQLNAEN
                     KIAEVTLILEPVSPVPQPEITFVFSAPWELDKFIQQLGISVAAQMPAGSWQGYELELS
                     QFTEQQWHDCQALLTRWQQEIEGKEFSDVLDIAKYGDRVIGIGEFEKISSPWLTKAQS
                     ENWLPDDIDFSAFSIETLSGWQPENPHHFDELQEKITQAEAAGETYITTPWNDSQLPL
                     DAAKTFSKNWEKQQITANEYQGNVADKTARAVLKIEQNIEETAYIKQRRNSLLNARHA
                     EPEIPLSLKEHIRLKDHQREGVAWLQQLFLRSPEETAGCLLADDMGLGKTLQILSFLV
                     WFIEKFPQEPPNLIVAPVSLLDNWERELNNFFYTAGIPVLKLYGETIKAVKYPKQAIP
                     AHLQSKGIKNLLKPGWQGEAKIILTTYETLRDQEFSLARQPWSIMVCDEAQKIKNPAA
                     LITHAANAVQARFKVACTGTPVENTLVDLWSLFDFAQPGLLGALNEFGKQYVRPIENE
                     DGRDTERLESLRALIEPQTLRRTKEEVARDLPQKIEVESCKQLTLSGVQKQLYLSSVA
                     NWQQQQALREGMQQAGTGMLGLLHRLKLICAHPAIVNPEPRFRDNSPKLNWLLKILTE
                     IKHTSKDKVIIFTELRDLQRELQHAIHQNFGFRPVIINGDSSTKSQSQNSRQRLIDDF
                     QAQPGFGVIILSTVAVGFGVNVQKANHVIHFTRCWNPAKEDQATDRAYRIGQTKNVYV
                     YYPTVRDTEITTFEETLDDLLQRRRALARDMLCATPDLNCADFETILKGA"
     gene            complement(8859..9025)
                     /locus_tag="ACHKIK_004264"
                     /pseudo
     CDS             complement(8859..9025)
                     /locus_tag="ACHKIK_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(9276..>9688)
                     /locus_tag="ACHKIK_004265"
     CDS             complement(9276..>9688)
                     /locus_tag="ACHKIK_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085321.1"
                     /translation="CKHSMCMEIYRLLSETQSMLAGYYWVMEYTQNKGLHIHFVGYLD
                     GQRHKNSYQISRQLGDIWRRITEGDGYFHLCRAKDKYPVRIDHVMHYSDKSAVDNLRY
                     ALSYLAKQDQKEHGIILGRSRLPEKSNRGRPRHN"
BASE COUNT         2731 a         2320 c         2263 g         2374 t
ORIGIN      
        1 acaccagaag aaggaggaga aaatgcttcc ctgtatattt attttgtcag gccatggcct
       61 ttctccagca accgatcaac gccacatacc tgcagcgaca ctcaacagaa gtgagatccc
      121 atgcaatatc atcatcacag ggccagcgga aggtcccttg tacgggcggt aggtacacag
      181 ccatatgcca tgtttatcct ggcacaggac ttttatgcaa gtataggctt tgttgatgca
      241 gaaccatcat accatgcaaa gctaagttcg ttctggacat actgtatcag gatgccaatc
      301 cccgacttcc atcgacaggt ttacgatcag gaatacatta tcaaccgtca gtattgcgaa
      361 actctcagaa gacatctgaa aacatatttc agaagagaat ccctgttcta catactttta
      421 gtcggtagcc tcatgcaggc tcgcacttta ccggcagcat tccataattt gtgatcctac
      481 tcattaagaa atcgttatca ggtattgttc atgaaaatcc tcaggatgga tcacaacgga
      541 agagtaatca tatcttatcg cagattcaga tttagaatgc ggcacgtaaa atattgtcca
      601 ggggacgctg accacgacac aacatcaaga aatttgcttt tgcatggtaa aaaagaggaa
      661 actaagaaaa tgtctcacct gattattctg tttctatcgt tcatttattg aacagcaaga
      721 gtattcaggt gtaaccgcac gacatagtga caaccaatgc aacataatca attatgttga
      781 ctatgttgct gatgactaat tttagtatgg ataatatatt aaacgagtaa aacaatgtcc
      841 tggcttaatt ccattttagt aacacttaca tctgtagaac cctataaggt acctgtcact
      901 gttatcgtca ctgttacctt tgcctttgtc tgctttattt tcttttattt actgagatcc
      961 atcagaatta tttatggatt aaaaaaatac acgcgttcaa ttaacagcat tgaaaaaagt
     1021 gcgccagagg ttcagcttga acaccttaag agcctgtttc agcggtctga actcaagcat
     1081 gcgtggaatg agttcgaaga atcgttgcac tcacaatatg aactggagaa cggcgaagag
     1141 aaaatcgtcc gtattcgggc tactgcgccg agcgccagct tcttctcgga gcaacaactg
     1201 gtcgatattc cgctgaacac tgaatttttc aaacacctgc ccggtattct gaccggtatg
     1261 ggaattatcg gtaccttcta cggtctgatg attggtctca accacttcga ccccagcaca
     1321 ccagagcagg tctccagtag cgtcaataat ctactacgcg atgtactgta cgccttcctg
     1381 ggttctgcat ttgctatttt tgcttccatt ctggtcacct ggcttgaaaa attatccatc
     1441 gctaaaagct acaaatatct ggaaaaattc accgcagccc tcgactccct gtatgacagc
     1501 ggcgttggcg aggagtacct tgcctcactg gtgaagtcca gtaatgaaag cgcgacccag
     1561 gcacgccatc tgaaagagag cctggttaca gatttacgcg atatgttact acatctggcc
     1621 gaaagtcaga aaatcgaaaa tgaacgcctc gcaaatacgt taagcgcaac ttaccgcgaa
     1681 tcaggttcac aatttgccga ccaggtcagc ggtgcgattg aaaacagctt gaagtcacca
     1741 ttggataaaa ttgccggtgc ggttcagacc gccagcggcg atcaaagcgg catggtacaa
     1801 aacatgctgc agaatgtact gaccgcgttt atggcgaaac tcgacaccac ctttggtcag
     1861 cagtttacaa acctcaacga aatgatgggg caaaccgttg gtgcaattca gaccatgcaa
     1921 accggtttct ccgcgttatt acaggacatg cgtcaggtga gcgacgattc ccgccagggc
     1981 agcgcgcagc ttatcgagca actgttatca gagatgaaat ctggccagca agctctacag
     2041 gctggcatga atgatatgct caccagcctt caagtctcag tggcgaaaat tggcgcagaa
     2101 ggtgaagggg ccggtgaacg tatagctcgc caactggaaa aaatgttcgc tgatagcgag
     2161 gcgcgagaaa aagcccaggc agaacatatg gctgcttttg ttgaagccat tcagaattca
     2221 gtacaacagg gacaaagcgc cacaatggaa aaaatggcag cctctgtcgg ggcgcttggt
     2281 gaacaactgg gtagcctgtt tggacagatc gataagggtc agcaacaaat ttcagcgact
     2341 cagcaagcga atcagcagtc cctgcacgaa cagactcaac gggtaatgag tgaggtagac
     2401 gatcagatta aacaattggt cgaaactgtt gccagccagc atcagggaac aacagaaact
     2461 cttcgtttgc tcgcagaaca aactaaccgg cagattcagg atatgcaagc tggcgcggac
     2521 aagatgcgtc tggctgctga acgctttgaa catgccggtg agcgggtgtc ggaagcaaac
     2581 caccttaccg ctgatgtgct gaataaagcg caatcggcag gttcatcact ttcccttgcc
     2641 accagcgagc tcacctccgt tgtggctgat tatcgtaaca atcgggaagc ggttagcaaa
     2701 tccattgcca tgctggagtt gctcgccgca aatacgcagt cagaacaaac cacgcgcaac
     2761 caattcatcg cagatcttaa gcaacacggt gagcgtctac agagctataa ccgggaagca
     2821 caggtcttta tggaaaatgt aagtgacgtg ctcgggaaag gatttgaaga cttctctgaa
     2881 ggtgtttcac gcagcctgga taaaacgctg ggtaaactgg acgtcgaaat ggcgaaagcc
     2941 tctaatctgc tcgcgggatc tgtcgaacag ctcggagaaa gcgtcagcga acttgatgat
     3001 gttctgtcac gcgtacgaac ctaaggggtg atggctgatg tttggaaacg catttggcgt
     3061 taaaaaacgc cgcagcgatg aagcagaaaa accattctgg atctcctatg ccgatctgat
     3121 gactgcaatg atggtgttat ttctggtcgt catggttgcg tcactgagtt ccgttacaca
     3181 gcgtattcaa cgtgcggagc aaggcgaaaa agcaaggggg caggatattt ccagattgtg
     3241 tgaacgcctg gaactgcatg cccgcaatgt gaacaaaaac atcgtagtgg attgccacga
     3301 taaccgtatc agctttggcg aagccgggcg tttcgctcat aaccaatttt tcctgaacgc
     3361 cgaggggcaa aaagcgttgc aggatgtagt tcctcttgta ctggaagcgt ccaacagtga
     3421 agagggtaag aagtggttta agcagatcgt tattgaaggg tttaccgaca ccgacggttc
     3481 gtacttatac aacctacatc tctccctgca acgctctgaa tgggtaatgt gtagtctgct
     3541 cgatagccgc agccctctgc aaaaaaatat ctccgcagaa cagcagctcc aaatccgtaa
     3601 gcttttcctc gcgggtggcg tctccttcaa taatgctaag gaaagcaagg aagccagccg
     3661 ccgggtcgag ttgcgcatgc agttcttcgg gcttaaagat aaacgtgata aggccgatga
     3721 ggtggatttt ccccccgtcg ttaataaaga agtttgccag ttggttatgc cactatgata
     3781 cccgccctga attctctttc tcaacgcatt gcagcgaggc tctcttccag ccaaagggat
     3841 gaccattatc tgcataatga tttccacgcg ttgaccgcgg ctactcttga catggaaaag
     3901 cggtttgata gggcagaaag aatcccctca ccgccacaag aaatgcggct ggaggctcta
     3961 cgtcgctttc gcctggcaca agaactcacg gaacgagagt ggcggatggt gttttatggc
     4021 ctcgccgata atgatccatt atatcctgac cagccgatcc tgctggagga tgatacattt
     4081 ttccccagag taaacagtgc aatcaaaaaa cgtattgaaa ccaaaacgtt gaaacgccgg
     4141 gattgggcag cactttgttc aagctatttt gcctaccaac accaatcacc cgagacaaac
     4201 cctcactggt gcgtactgcg ggggcatatc gctcagggtt acctggtagt gaaagccgct
     4261 atacgacgag aaaaatcgtg gatgaaaacc attgagtttt atcacgacat ttttacccca
     4321 caagcgggag gagtgatctc caggcagtta cttgcaggtg agaacaattc actctcatca
     4381 ttagagaaaa tcgcgcaaat ccctgatagc agttggttat ggaagcgcat tttcactgtg
     4441 cttttagctc aactggacac gctcgacgat ccccagtttc tgagcaaaat tagctggttg
     4501 ttaggcttag ctgcgcagtg ggtgcgtttc cgtgatgaca tcatgactgc aaccctgact
     4561 cgctattacc attctggcta tcgcgatcag tcacatccag cgttgaagca ggccgcgctg
     4621 gaatactggg ataaccccca gttgaaaagc cagcaaaata aatggcatca gtatgtctcc
     4681 gagtcggttg ccgcaatggt ccggggctgg ttggcaaaac aggacctgac acatttcttt
     4741 gaactgctgc gcggtaatgg cgatgtcgat caggctcgac tgcactactg gctgcgtttc
     4801 gctaatcaga tgggatttac tcgtatcatc atggggtccg atgcctggca agatcgtggt
     4861 agcgattttg tgaaattccg cgaggagaat aagggacgat tgagttactt acgaggtggg
     4921 cgtaactttg ataacgccat ggttatgcaa atcaacgatt atctgtttgt cgagttttcc
     4981 gggacaggaa atgcgatgta tgcctatcaa ataggtcacg cgccatttaa ccctgaatca
     5041 cgcacgcttg atatcaacat tcatctaaaa gatcaggggc gatgcgcact cagactcccc
     5101 catgcacctc gtgctgaggg gtataacaaa gtccggataa ccggctggat gttgaaatac
     5161 gacgatgagc tacgtaagtt gggcattcgg tggatggctg aagaacccgt caggtttgtc
     5221 gataagaaag tgccccctcc cgtcgctatg tctgacatca aaatcatcaa cccgttgcgt
     5281 gatacggcta tacaacgcct ggttaaatgt agttcatgta tcgtcagcga taatcgacat
     5341 aaaggtggca tactgtccgt acaactcatt acacctgacg acaccgtaga aagagagttg
     5401 ttacgactag gctttgcacc tgtagctaaa gaaccgcatc gttactggat caaataatgc
     5461 tgaaacgtct gttaagtaaa ctgacgggaa accgtcagca gatagaacac cacctaaaga
     5521 atcagtatca ggttgaggaa aacggtctca gctttccgct atcgctggtt gatgattcac
     5581 agctttgggc actggcatcc tggcttgaac aacttgctga agaagactat ctgatatcac
     5641 ttacagatcg gtggttattg agttgggagg cactatatcg cctgctggag gatgaggaac
     5701 atgccagcag cctgcctctt atcggtgtac ctgacatatt gcccttacgc gcaagcctga
     5761 gttcacgagg agcattaagc gatagcgatt ttcgcgtctg gattgctgaa tgggctacat
     5821 ttccagcccg caagccgatt cgtttcagtc gtaccggtgc cattttaacg catgataatc
     5881 agcaatacct gttatcgcgg gaaaactggg cgctcttaca ggcaacagaa caactcagtg
     5941 cacagcaaat tcagactccc ggcgaaacca ctaaccagct gggatgggcc gctatccgca
     6001 aatgtgcgaa actcgccgcc gccaaatttg atgattattt agaaaaaacg catgtcatca
     6061 aaccaacttc attgtcatta cgtctgcgta aagcaacggt tgccgatact gctgttatcg
     6121 aaattgagcc ccatttcgag gaccagcctg ctaactggct gggcagtttt gacaagaact
     6181 tacaggttca cgatagctac cgcatcccag gagagaatgg cgaactcagt cacgtcatca
     6241 tccctcccga agtgaaagaa gtcctgaatt caatacactc aatcccaggg cgtcgagtgg
     6301 ccggaagtga agcactttcc tttgttcgca atccttatac cttcctgggt gaggatgccg
     6361 ccagtgttat tgcccctgaa gaacatgaac aggcactgtt tgatgccagg attttctttc
     6421 atcatttcag gttgataccg caactgaatg cagaaaataa gatcgcagaa gttacgctga
     6481 tacttgagcc tgtttcacct gttccccagc cagaaatcac ttttgtgttc tcagcgccct
     6541 gggagctgga taaattcata cagcaactcg gtattagcgt cgctgcacag atgcctgccg
     6601 gttcatggca aggttacgag ctggaattaa gtcagttcac cgaacagcag tggcacgatt
     6661 gccaggcact gctaactcgc tggcaacaag aaattgaagg aaaagaattt agcgatgtgc
     6721 tggatattgc gaagtatggc gatagagtga ttggcattgg tgaatttgaa aaaatatcct
     6781 ctccctggct gaccaaagcg cagagtgaaa actggcttcc tgatgacatc gatttctccg
     6841 cgttttcaat cgaaacacta tctggctggc aacctgaaaa tcctcaccat tttgatgaac
     6901 tacaggagaa aattactcag gcagaagccg ctggtgaaac gtatatcacc acaccatgga
     6961 atgacagcca attaccgttg gatgccgcta aaaccttcag taagaactgg gaaaaacagc
     7021 aaatcactgc taatgaatat caaggcaacg ttgcagataa aacggcccga gccgtactca
     7081 aaattgagca gaatattgaa gaaacggcct acatcaaaca gcgccgcaat tcactcctca
     7141 atgcacgtca tgccgaacca gaaattcctc tgagccttaa agagcatatc cgacttaaag
     7201 accatcaacg tgaaggcgta gcctggctcc agcaactctt ccttcgttca cctgaggaaa
     7261 cagcaggctg cctgctggcg gatgatatgg ggcttggaaa aacgctgcaa attctgagct
     7321 tcctggtatg gttcattgaa aaattcccgc aagaaccgcc aaatctcatt gttgctcctg
     7381 tctctctttt agataactgg gaacgtgagc tgaacaattt cttctatacc gcaggaatac
     7441 cagtattgaa attgtatggc gagaccatta aagcggtgaa atatcccaaa caggccatcc
     7501 ctgctcatct gcagtctaag gggatcaaaa acctgcttaa acctggctgg caaggtgagg
     7561 caaaaatcat cctgacgacc tacgaaacgc ttcgcgacca agagttttct ctggcacgcc
     7621 agccatggtc cattatggtg tgcgacgagg cacaaaaaat aaaaaaccca gctgcgttaa
     7681 tcacacatgc ggccaacgcg gtacaagcca ggtttaaagt ggcatgcaca ggaacgccag
     7741 tcgaaaacac acttgtcgat ctgtggagct tatttgattt tgcccagcca ggattgttag
     7801 gggcactaaa tgaatttggt aaacagtatg ttcgccccat cgagaacgaa gatggccgcg
     7861 atacagaacg gctggaaagc ctgcgggcgc tgatagaacc tcagacttta cgccgaacca
     7921 aagaagaggt tgcacgtgat ttacctcaga aaattgaagt agaaagctgc aagcaactga
     7981 cgttgtctgg cgtgcaaaaa cagctctacc tttcgtctgt tgcaaattgg caacaacagc
     8041 aagcactgcg cgaagggatg cagcaggccg gaaccggtat gttaggtttg ttacaccgac
     8101 tgaaactcat ctgtgcacac cctgcaatag ttaatccaga gccacgtttt cgtgataact
     8161 cacccaagct taactggctg ctgaaaatac tcacagaaat aaaacatacc tctaaagata
     8221 aggtcattat ttttacggag cttcgagatt tacagcgcga gcttcaacat gcaatccacc
     8281 aaaattttgg tttccgccct gtcattatca atggtgatag cagcactaaa agtcaaagcc
     8341 aaaacagccg ccaacgcctg attgatgatt ttcaggctca gcctgggttt ggggtgatta
     8401 ttctctctac ggttgctgtc gggtttggcg ttaacgtcca gaaagccaat catgttattc
     8461 attttactcg ctgctggaat ccagccaaag aagatcaggc tacggatcgg gcgtatcgca
     8521 ttgggcaaac taaaaatgtg tatgtctatt acccaactgt gagagatact gaaatcacaa
     8581 catttgaaga aacactggat gatctcttac aacgccgacg agcattggcc agagatatgc
     8641 tctgcgctac tcccgatttg aactgtgcgg attttgagac cattttgaag ggagcctaac
     8701 cgatatgagc ggcaatacta tcagttgcca aacctgaaag aacacttagc tgtgatgatt
     8761 cgctattacc taccgatagc ttcggaaaaa cataaattaa aaatgcccag aggagtacta
     8821 ttatcgttag tattattatc cgaacgctgt attaacggtt atttattggt gagtactacg
     8881 ttccatggca agagttcatc aacaggttgg aagaccattc cggcaaaact ctcagtatat
     8941 ggcgcagata ccattcagac agcaggtccc gctcagcccg tacagtagag cgccgttcac
     9001 taccgtaatc gctaccgaag aacacattga ttgcccctca ataatcctgc tgttaacatc
     9061 aaaattgttg gtcgggttcg atttatttaa ttgtgtaaac agggtctctc agagaaaggc
     9121 ccttatgcat cacctgacgt taaaagtcac tacacccaag atgacgttca aagtgtcata
     9181 gtcccctaca ctgatgacaa agccgccctc tgctgcctat taactcaagc acctcccaac
     9241 aacttacaga aacttaattc taatacagct gtaattcagt tgtgtcgtgg tcggccgcgg
     9301 ttactttttt caggaagcct gctacggccc agaataatac cgtgttcttt ctgatcctgt
     9361 ttagccagat agctaagggc gtaacgtaag ttgtctaccg cggacttatc actatagtgc
     9421 atcacgtgat caatacgcac cgggtattta tctttagccc ggcacaggtg aaaataacca
     9481 tccccttccg tgatccgcct ccagatatct cccagttgcc tggatatctg ataagagttc
     9541 ttgtgacgtt gcccatccag gtagccgaca aaatgaatat gaagcccttt gttttgtgta
     9601 tattccatta cccagtaata tcccgccaac atcgattggg tttcgctaag taagcggtat
     9661 atttccatac acatactgtg cttacagc
//
LOCUS       DAZGJW010000167         9574 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZGJW010000167 DAZGJW010000000
VERSION     DAZGJW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.167"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            133..1215
                     /locus_tag="ACHKIK_004266"
                     /pseudo
     CDS             133..1215
                     /locus_tag="ACHKIK_004266"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
     gene            1381..1659
                     /locus_tag="ACHKIK_004267"
     CDS             1381..1659
                     /locus_tag="ACHKIK_004267"
                     /inference="COORDINATES: protein motif:HMM:NF045049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085322.1"
                     /translation="MRRLGARICEYCAKTGRCEVHHVRKLKDLKKGRKAGYKPSLWQL
                     MMIARRRKTMILCASCHDKLHSGKLPDLRQEKGRILLQPPVSSGDSAK"
     gene            1809..2294
                     /gene="ssb"
                     /locus_tag="ACHKIK_004268"
     CDS             1809..2294
                     /gene="ssb"
                     /locus_tag="ACHKIK_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO9085323.1"
                     /translation="MGKDPEVRYIPNGGAVANLQVATSESWRDKQTGEIREQTEWHRV
                     VLFGKLAEVAGEYLHKGEQVYIEGQLRTRSWEDNGITRYVTEILVKTTGTMQMLGRAA
                     GTQTQPEEAQQFSGQPQPESQPEPKKGGAKTKGRGRKAAQPEPQQPSEPAYDFDDDIP
                     F"
     gene            2350..2583
                     /locus_tag="ACHKIK_004269"
     CDS             2350..2583
                     /locus_tag="ACHKIK_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO9085324.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHAIRDFGILK"
     gene            2642..4606
                     /locus_tag="ACHKIK_004270"
     CDS             2642..4606
                     /locus_tag="ACHKIK_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO9085325.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERDIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEFLREAEGWGWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDAADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECEHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDPLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCTPRPQAETETTEYRDDQAEAA"
     gene            4675..5109
                     /gene="psiB"
                     /locus_tag="ACHKIK_004271"
     CDS             4675..5109
                     /gene="psiB"
                     /locus_tag="ACHKIK_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO9085326.1"
                     /translation="MKTELTLNVLQTMNAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVNDIIHILMAEGGQA"
     gene            5106..5825
                     /locus_tag="ACHKIK_004272"
     CDS             5106..5825
                     /locus_tag="ACHKIK_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO9085327.1"
                     /translation="MSVRSRALVPLCTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGSRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREVEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            5822..6136
                     /locus_tag="ACHKIK_004273"
     CDS             5822..6136
                     /locus_tag="ACHKIK_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="theronine dehydrogenase"
                     /protein_id="HFO9085328.1"
                     /translation="MKWRYSLRWKLPSPCPGEHELVSEVVEAGQPAPASVMSRRVAGA
                     GYAVCLDFISDRQVRRWSEERKAAVRRRNLEKRINRHAPLFADELIARELAERPDYFQ
                     GK"
     gene            6286..6438
                     /locus_tag="ACHKIK_004274"
                     /pseudo
     CDS             6286..6438
                     /locus_tag="ACHKIK_004274"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            6383..6505
                     /locus_tag="ACHKIK_004275"
     CDS             6383..6505
                     /locus_tag="ACHKIK_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFO9085329.1"
                     /translation="MVCCTLLIFTLLTRNRLCEIRLKDGYREVTASMAYESGGK"
     gene            complement(6743..7103)
                     /locus_tag="ACHKIK_004276"
                     /pseudo
     CDS             complement(6743..7103)
                     /locus_tag="ACHKIK_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7404..7691
                     /locus_tag="ACHKIK_004277"
     CDS             7404..7691
                     /locus_tag="ACHKIK_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085330.1"
                     /translation="MSTRNIHVNTASYTILVAGREKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            7810..8631
                     /locus_tag="ACHKIK_004278"
     CDS             7810..8631
                     /locus_tag="ACHKIK_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO9085331.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPELIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSVSGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(8927..9496)
                     /locus_tag="ACHKIK_004279"
                     /pseudo
     CDS             complement(8927..9496)
                     /locus_tag="ACHKIK_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297767.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
BASE COUNT         2413 a         2335 c         2680 g         2146 t
ORIGIN      
        1 catatccctc cggcagaagc agaaaaagct tattatgctt ccatcagaaa cgatgatctg
       61 gcagcctgag ttcacagata aaacactctc caggaaaacc ggggcggttc aagggtttat
      121 gaaccagtat tctctgaaca ctctcatgga ttcagaccaa aacgttcgtg tgaaactgcg
      181 ctcagatcta ttcgtccagt ctggaacgga gttaaatgga tcgtggatgt tgatatcaaa
      241 ggtttctttg acaatatccc ccacaaacta ttgctgaata cactggcaga gaaaattaag
      301 gataagaatt tcctgaaatt aatcgaacag tggctgaagg caggatatgt ggacaattgg
      361 aaataccatc gctcatattc tggcacgcca caaggtggga taatttctcc cttactggca
      421 aatatatacc tcgacaaact ggaccgtttt gtagaacaga acttaattcc tgcatataca
      481 agcggtacaa aacgaagagc gaatccagat atgaacaggc tggcccataa gattcataaa
      541 ctacgaaaga aagtagacgg tatggcaact gattctgaat cagaaaaaga agccataaag
      601 aaagaaattg aacgactgct tgaagaaaaa cgttctatac catctcaggt catgaacgat
      661 ccaaacttca ggcgtatgta ctatgttcga tatgctgatg acttcgtaat tggtgtcatc
      721 ggctcgaaga aagatgctga acatatatca agacaggtca gaaactttat tactacttca
      781 ctgggattgg aagttaatga agcaaagacc aggatccgcc atatctcaga aggagttaat
      841 tttttaggct atgaaatcag acaggcagat gcaaagaaat tgctgaagca gaaaatgcaa
      901 gggcggcacg ctctccggag atccaccaca ggaattgtgc agctcttcgt tcctgacaat
      961 atagccgcta aattttgtca ccaaaagaaa tatggctgtt atgagaatgt gaaggctgta
     1021 catcgttcaa gtctacaaaa tctcagtgaa gctgaaattg ttctgacttt caacgcggaa
     1081 atgcgcggat tggcgaacta ctacagtctg gcgctggaca tgaaatataa attatcaaaa
     1141 ttatatttca tatggcaaat aagccttttt aagaccctag cgaataaacg acgtagttca
     1201 gtgaataaag tcgccaaatc gctacggcaa aataatggag atctggctat taccgttcag
     1261 gccaaaaatg gatcccgaaa gattgaggtt ttcaaactca aacatgtcaa tcgcaaccga
     1321 acaacgattg tcgatacgga accgagaacc gcacatatca caacccgcac aacagagatc
     1381 atgcgtcgat taggggcaag gatctgcgag tattgtgcga agactgggcg ctgcgaagta
     1441 catcatgtca gaaagctaaa agacctgaag aaaggtcgta aggccggata taaacccagc
     1501 ctgtggcaac taatgatgat cgcacgccgc cggaaaacga tgattttgtg cgcaagttgc
     1561 cacgacaagt tacatagcgg aaaactaccc gatctgagac aggaaaaggg aagaatactc
     1621 ctgcaaccac ctgtctcaag tggggattct gctaaatgag tttatcccca ataagctagc
     1681 aatggagagc cgtatgcatt gaaaggtgca cgtacggttc ggaggggggg taacggtgat
     1741 ctaactcaag ataagcagga catattcgcc atttatcttg aaagaccaca cgtaccctac
     1801 cctaccgtct gggaaaagat ccggaagtcc gttacatccc aaacgggggc gcagtggcaa
     1861 acctgcaggt ggccacgtca gaaagctggc gtgacaaaca gacgggggag atacgggagc
     1921 agacagaatg gcatcgcgtg gtgctgttcg gcaagctcgc ggaagtggcc ggtgaatatc
     1981 tgcacaaggg cgagcaggtc tacatcgagg ggcaactccg tacccgtagc tgggaagata
     2041 acggtatcac ccgttacgtc actgaaattc ttgttaagac cacgggcacc atgcagatgc
     2101 tgggacgtgc cgcaggtact cagactcagc cggaagaggc gcaacagttc agcggtcagc
     2161 ctcagccgga atcacagccg gaaccgaaaa aaggaggcgc gaaaacgaaa ggccgtggac
     2221 gtaaggccgc gcagcccgag cctcagcagc cgtctgaacc cgcgtatgac ttcgatgacg
     2281 acattccgtt ctaacgactg acagtgacaa ccgccccgtc cagtgcgggg catcaccgga
     2341 gagatgagga tgagcgaata tttcagaata tttcaggggc tgcctgacgg ctcctttacc
     2401 cgcgaacagg ctgaagccgt tgccgcacag taccggaatg tctttatcga ggatgaccac
     2461 ggcgaacagt ttcgcctggt tgtccgtaat aacggaacaa tggtctggcg tacctggaat
     2521 tttgaggacg gtgccgggta ctggatgaac catgccatca gggatttcgg gattcttaag
     2581 taagagaggt gccggacgcg cgaacgtccg gcaggacata agcaattata aggggatgat
     2641 tatgcctgta acgaagtgtg aaccagaaac cacccgcaaa gcaagccgta aatctgtaaa
     2701 aacgcaggaa actgtcctgt ctgccctgct ggcgcagacg gaggaagtga gcgtgccgct
     2761 ggcctcactg attaaatcac cgctgaatgt gcgcacggtg ccgtattctg cggagtccgt
     2821 cagcgaactg gctgagtcca ttaagggcgt tggcctgctg cagaatctgg tcgtgcatac
     2881 cctgcctggt gaccgttacg gtgtcgccgc aggtggtcgc cgactggcag cactcaacat
     2941 gctggcagag cgtgacatca ttccggctga ctggcctgtc cgtgtgaagg tcattccgca
     3001 ggagctggcg actgccgcat cgatgaccga gaacggtcat cgtcgggata tgcaccctgc
     3061 cgaacagatt gccggattcc gtgcaatggc gcaggaaggc aaaacacctg cacaaatcgg
     3121 tgatttgctg ggttattcgc cccgccacgt tcagcgaatg ctgaaactgg cagaccttgc
     3181 gcctgtcatc ctcgatgcgc tggcagaaga ccgcatcacc accgagcact gtcaggcgct
     3241 ggcgctggag aacgacaccg cgcgtcaggt gcaggtgttt gaagccgcct gtcagtcggg
     3301 atggggcggt aagccggaag tacagaccat tcgtcgtctg gtgaccgaaa gtgaagtggc
     3361 ggtggcaggg aacagtaaat tccgcttcgt gggggctgat gccttctcgc cagatgaact
     3421 gcgcaccgat ttgttcagcg atgacgggga cggttatgtc gaccgcgtgg cgctcgatgc
     3481 cgccctgctg gaaaaactcc aggctgtcgc agagttcctt cgggaagccg aaggctgggg
     3541 atggtgcgcc ggacgcatgg agcctgtcgg tgagtgccgt gaggatgccg gaacataccg
     3601 ctgtctgccg gagccggaag cggtgctgac ggaggcggaa gaagaacgtc tgaacgaact
     3661 gatggctcgt tacgacgcgc tggaaaacca gtgtgaggaa tctgacctgc tggaagcaga
     3721 aatgaagctg atgcgctgca tggcgaaggt cagagcgtgg acgccggaga tgcgtgccgg
     3781 aggtggagtg gtggtgtcct ggcgttatgg aaacgtatgt gtccagcgtg gtgtgcagtt
     3841 gcgcagtgag gatgatgcgg ctgacgacgc tgaccgcacg gaacaggtgc aggaaaaagc
     3901 gtcagtggag gaaatcagtc tgccactgct gacgaaaatg tcttccgaac gcacgctggc
     3961 agtccaggcg gcactgatgc agcaaccgga caaatccctg acactgctgg catggacgct
     4021 ctgcctgaat gtgtttggca gcggagcgta cagtaaacca gcacaaatca gcctggaatg
     4081 tgaacattat tcgctgacca gcgatgcgcc atcggggaag gaaggtgccg cattcatggc
     4141 gctgatggca gaaaaagccc gtcttgctgc cctgttaccg gagggatggt cacgggacat
     4201 gacgacattc ctgtcactca gccaggaggt actgttatcc ctgctcagtt tctgcactgc
     4261 gtgcagcatc cacggcgttc agacccgcga gtgtggtcac acgtcacgca gtccgcttga
     4321 cccgctggaa accgctatcg gattccacat gcgcgactgg tggcagccga caaaagcaaa
     4381 cttcttcgga cacctgaaaa agccgcagat tatcgcagcc ctgaatgatg ccggactgtc
     4441 cggtgccgca cgggacgcag agaagatgaa gaaaggcgat gccgctgaac atgcagagtt
     4501 ccacatgaaa gacaaccgct gggtgcctgg ctggatgtgt acaccacgtc cacaggcaga
     4561 gacggaaacc acggagtacc gtgacgacca ggcagaagcc gcctgaccgc cagtcataaa
     4621 tcatttatca cgccgccccg ccggagacgg ggcggcagca agggagatac cgtgatgaaa
     4681 actgaactga ccctgaatgt attacagacc atgaacgcac aggaatatga agatatccgg
     4741 gctgccggaa gtgatgaacg ccgtgagctg actcacgccg tgatgcggga actggatgcg
     4801 ccggataact ggacgatgaa cggagagtac ggcagcgagt ttggcggctt tttccccgtc
     4861 caggtccgtt tcacgcctgc ccacgaacgt tttcacctgg cgttatgttc accgggcgat
     4921 gtctctcagg tctgggtgct ggttctggtg aatgccggtg gtgagccgtt tgcggtggtt
     4981 caggtgcagc ggcggtttgc accggaagcc gtcagccatt cactggcact ggcggcgtca
     5041 ctggatgcgc aggggtacag cgttaatgac atcatccata tcctgatggc ggaaggaggt
     5101 caggcatgag cgtacgttca cgggcactgg ttcccctctg cacagagcaa caggccgcat
     5161 ggcgggcggt ggcggagacg gaaaaacgcc gccatcaggg aaacacgctg gcggaatatc
     5221 cgtatgcggg ggcgtttttc cgctgtctga acggaagccg caggatttcg ctgtctgacc
     5281 tgcgattttt catgccatcc ctgaccgcag aagaactgca cggcagccgt cttcagtggc
     5341 tgtacgccat cgatgtgctg attgaaacac agggcgaggt ctgcctcctt ccccttccag
     5401 gcgatgcagc agaacgcttg ttcccgtcgg tgcggtttcg tgtcagggaa cgcagtcgcc
     5461 ataaatccgc gctggtcatg cagaagtaca gccgccagca ggcgagggag gtagagcaga
     5521 aagcccgtgc gtaccaggcg ctggtcgctc aggctgaaat cgaactggca ttccattcgc
     5581 cggaaacagt cggaagctgg cacgcccgct ggagtgaccg ggtggcagag catgacctgg
     5641 aaaccctgtt ctggcagtgg ggcgagcgtt ttccgtcact ggccggaatg gaacgctggc
     5701 agtggcagga tatgccgttc tggcaggtta ttgcggaagc cagtcttgca gcaagagagg
     5761 ccggtcacgc tgtccgtgag atggagcggt ggatggtgcc gaacaaactc cgggaggcgg
     5821 cgtgaagtgg cgttactcac tgcgctggaa actgccttct ccctgtcctg gggagcatga
     5881 actggtttca gaagtggtgg aggcggggca gcctgcaccg gcatccgtta tgtctcgcag
     5941 ggttgccggt gcagggtatg cggtctgtct ggattttatt tctgatcgcc aggtgcgccg
     6001 ctggagcgaa gaacgtaagg ctgcggtacg gcggcggaat ctggagaaac gtatcaacag
     6061 gcatgcacca ctgtttgcgg atgaactgat tgcccgtgaa ctggctgaac gaccggatta
     6121 ttttcaggga aagtgagtgt ggtcagcgcg cagggatatg ggctatgatg tgcccggcgc
     6181 ttgaggcttt ctgcctcatg acgtgaaggt ggtttgttac cgtgttgtgt ggcagaaagc
     6241 agaaagcccc gtagttaatt ttcattaacc cacgaggccc gctgtatgtc tcatccacgt
     6301 cagtatggcc tcttaccgtg caaattgcaa ggaggagtaa accatgaaac taccgggaaa
     6361 cgcccttatc tggtgcgtat taatcgtgtg ctgcacgttg ttgatattca cactcctgac
     6421 ccggaatcgc ctgtgcgaaa tacggctgaa ggacggatac agggaggtta cggcaagcat
     6481 ggcttacgaa tccggcggta agtaacaacc tggaggcggg cgcaggcccg ccttttcagg
     6541 gctgatgctg gtctgactgc actgaagcgc ctttataaag gggctgccgg ttcgccggtg
     6601 gcccctttct ccttgctggc attgtgcggg catgaacaac cagactgcca ggaaggccgt
     6661 ttccctccgg gaacggggat gaacgtgcgc ctgcggcgcc cgttcttttc ccccgccttc
     6721 tctgtttatg gcctgtcgga aattacatca ccttccctgt gattattctc tttttcctac
     6781 tgttctgact ctgattactg ctgccctgat tattgtctgt tttgttgttc tcctggcagt
     6841 gccttttccg ggcgtccccc tggctgggcg ggctgtcgtg aacggagcca gcagagctgt
     6901 ctctgccctg gcgggcttcc atccctgagg cgaaaccccc ggcttgcagc gaccggaacg
     6961 gtcgcgccgc ccttgtcgcg ccgtggcctg cgctcctggc atcccggcaa aaagacgccg
     7021 ggatgtaagg cgcaacgccg tttcgcaaac cgcgcttcac gtcgttgcgg cgggtgcctg
     7081 cccgttgttg tgtccggtgc cattcacctg tttaagcccc tgtcgccggg ggctgataca
     7141 gccacaacct gccgcactga ctgtccaggg taaatgaaca gcccacaaaa ttttttaccc
     7201 aaaacccggt aaaaaatttt gcgttctgcc caggacaggt gcgtcaggcc gtggcagtga
     7261 tgccccatgc ggcgaaggtt ctcaaacagg tgatggtgaa taccggagga aacaaagagc
     7321 aggtccccgc aaaacgtcaa aaccgcggct cccgaaactc aacagatgat gggggctgaa
     7381 aaccagagag aggagaaagc tggatgagta cccgaaatat ccacgttaac accgcgtcgt
     7441 acacaattct tgttgccggg agggaaaaaa acaccgggga agagtgggac gtcctggaat
     7501 tcagcagcct gactgagctg aaaaaatatc gcaaaagcca cccggaaaag atggccttca
     7561 gctatagcta cgcgctcagc cggggtgtgg atacgcagtt ccgccatatc aacattgcgg
     7621 aagccgatca cttcaaacag ttcctgcgtc agattaagcg tgccggcctc gatatccggg
     7681 cgatctgctg accggatgtg atgaatatcc ctcctgattg ttgcaatctt gttaatggag
     7741 atcgggaggg atgatattaa gcggaaaaat tttttattaa atttattctt tcataaagga
     7801 gtagctgtta tgcgactagc ttcccgtttt ggtcggtata attccatccg tcgtgaacgt
     7861 cctttaacgg atgatgaatt aatgcagttc gtgccttcgg tattttccgg tgataaacat
     7921 gagtcccgga gtgaacgtta tacgtatatt ccaacaatca atatcatcaa taagttacgt
     7981 gatgaaggtt tccagccatt ctttgcctgt cagagtcggg ttcgtgattt gggacgccgc
     8041 gaatacagta aacatatgtt acgtcttcgc agggaagggc atattaacgg gcaggaagtt
     8101 cctgaaatta tcctgcttaa ttcacatgat ggttcatcca gttatcagat gatccccgga
     8161 atttttcgtt ttgtctgcac aaatggcctg gtgtgcggaa ataattttgg cgaaatccgt
     8221 gttccacata aaggtgatat tgtcgggcag gttatcgagg gagcgtatga agtgctcggt
     8281 gtctttgata aggtcactga taatatggag gcgatgaaag aaattcatct taacagtgac
     8341 gagcaacatt tatttggcag agctgcactg atggtcaggt atgaagatga aaataaaacg
     8401 ccagtgacgc ctgaactaat aattactccc cgtcgtcggg aagataaaca gaacgatctc
     8461 tggacaacct ggcagcgggt tcaggagaat atgataaaag gtggattatc ggggcgaagt
     8521 gtctccggga aaaataccag gacaagagcc attacaggta ttgatggtga tataagaatc
     8581 aacaaggcgt tatgggtgat tgccgaacag ttcagaaagt ggaagtcatg ataatataag
     8641 taaagcccag aaaaattttc ggggctttac ttatattatc attcttcggc atcgctgtgt
     8701 cgtctgatat aactcaattt catcaactgg ctttccagtt gtctgtaaat attgtgcccg
     8761 cgaaaaatgg tctggcagga ggctgcgcag ttaccgggta tatataagac gggttcccgc
     8821 ttcgcggggc ttcggccagt cagtattgtc actcatatga tatttttgtg tgtggccttc
     8881 catgccgctt ttgcggcata accggtatct gacaatgtct gtaagattaa tttttctgca
     8941 cactgttaat ttccggaagt gatttcttcg tggccggaat ccggatacct ttactgcttt
     9001 ttatcccggt ataaatccgg taaacctctg atgcgtaggc aagacgtctc tggttctggc
     9061 gttcggtctt cctgaatccg gcattgtatg caccaacggc ctcccaggag acgccccatt
     9121 ttttaaaggc tattgccaga taataagcac cggtataaat gttcatgcag ggatctgttg
     9181 tcagatgttc cggcttaatt ccatagcggg ccagttcgtt aaaatgctgg gaatctacct
     9241 gcatcagtct gctgccatat cccgttaccg gattaatacc gatggcatta acccggtaaa
     9301 gggattcttt ccatgatatc gctctcagta aatccgggtc tattttgtaa tcccgacctg
     9361 caagatcaaa gcaatcggcg gcttcgcagc tcccatttat aaacatcagg cagattgcta
     9421 acatccactt tttcattttt tcacctctgg tgacattatc cggaaataat ttaacctact
     9481 ccacaataag gctcaactag ttggtggttc tcaccaccaa aagcaccaca cactacgcaa
     9541 aaacaagttt ttgctgattt ttctttataa atag
//
LOCUS       DAZGJW010000168         9273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZGJW010000168 DAZGJW010000000
VERSION     DAZGJW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.168"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            182..1990
                     /gene="frdA"
                     /locus_tag="ACHKIK_004280"
     CDS             182..1990
                     /gene="frdA"
                     /locus_tag="ACHKIK_004280"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO9085332.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            1983..2717
                     /gene="frdB"
                     /locus_tag="ACHKIK_004281"
     CDS             1983..2717
                     /gene="frdB"
                     /locus_tag="ACHKIK_004281"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO9085333.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2728..3123
                     /gene="frdC"
                     /locus_tag="ACHKIK_004282"
     CDS             2728..3123
                     /gene="frdC"
                     /locus_tag="ACHKIK_004282"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO9085334.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3134..3493
                     /gene="frdD"
                     /locus_tag="ACHKIK_004283"
     CDS             3134..3493
                     /gene="frdD"
                     /locus_tag="ACHKIK_004283"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO9085335.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            3556..4689
                     /locus_tag="ACHKIK_004284"
     CDS             3556..4689
                     /locus_tag="ACHKIK_004284"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO9085336.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            4778..5311
                     /gene="blc"
                     /locus_tag="ACHKIK_004285"
     CDS             4778..5311
                     /gene="blc"
                     /locus_tag="ACHKIK_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO9085337.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(5308..5625)
                     /gene="sugE"
                     /locus_tag="ACHKIK_004286"
     CDS             complement(5308..5625)
                     /gene="sugE"
                     /locus_tag="ACHKIK_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO9085338.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(5807..5953)
                     /gene="ecnB"
                     /locus_tag="ACHKIK_004287"
     CDS             complement(5807..5953)
                     /gene="ecnB"
                     /locus_tag="ACHKIK_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO9085339.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(6064..6189)
                     /gene="ecnA"
                     /locus_tag="ACHKIK_004288"
     CDS             complement(6064..6189)
                     /gene="ecnA"
                     /locus_tag="ACHKIK_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO9085340.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(6241..6807)
                     /gene="efp"
                     /locus_tag="ACHKIK_004289"
     CDS             complement(6241..6807)
                     /gene="efp"
                     /locus_tag="ACHKIK_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO9085341.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            6849..7877
                     /gene="epmB"
                     /locus_tag="ACHKIK_004290"
     CDS             6849..7877
                     /gene="epmB"
                     /locus_tag="ACHKIK_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO9085342.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            8267..9136
                     /locus_tag="ACHKIK_004291"
     CDS             8267..9136
                     /locus_tag="ACHKIK_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO9085343.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         2367 a         2345 c         2381 g         2180 t
ORIGIN      
        1 cagtgataat ttagccctct tgcgcactaa aaaaatcgat ctcgtcaaat ttcagactta
       61 tccatcagac tatactgttg tacctataaa ggagcagtgg aatagcgttc gcagaccgta
      121 actttcaggc acttaccctg aagtacgggg ctgtgggata aaaacaatct ggaggaatgt
      181 cgtgcaaacc tttcaagccg atcttgccat tgtaggcgcc ggtggcgcgg gattacgtgc
      241 tgcaattgct gccgcgcagg caaatccaaa tgcaaaaatc gcactaatct caaaagtata
      301 cccgatgcgt agccataccg ttgctgcaga agggggctcc gccgctgtcg cgcaggatca
      361 tgacagcttc gaatatcact ttcacgatac agtagcgggt ggcgactggt tgtgtgagca
      421 ggatgtcgtg gattatttcg tccaccactg cccaaccgaa atgacccaac tggaactgtg
      481 ggggtgccca tggagccgtc gcccggatgg tagcgtcaac gtacgtcgct tcggcggcat
      541 gaaaatcgag cgcacctggt tcgccgccga taagaccggc ttccatatgc tgcacacgct
      601 gttccagacc tctctgcaat tcccgcagat ccagcgtttt gacgaacatt tcgtgctgga
      661 tattctggtt gatgatggtc atgttcgcgg cgtggtagca atgaacatga tggaaggcac
      721 gctggtgcag atccgtgcta acgcggtcgt tatggctact ggcggtgcgg gtcgcgttta
      781 tcgttacaac accaacggcg gcatcgttac cggtgacggt atgggtatgg cgctaagcca
      841 cggcgttccg ctgcgtgaca tggaattcgt tcagtatcac ccaaccggtc tgccaggttc
      901 cggtatcctg atgaccgaag gctgccgcgg tgaaggcggt attctggtca acaaaaatgg
      961 ctaccgttat ctgcaagatt acggcatggg cccggaaact ccgctgggcg agccgaaaaa
     1021 caaatatatg gaactgggtc cacgcgacaa agtttctcag gccttctggc acgaatggcg
     1081 taaaggcaac accatctcca cgccgcgtgg cgatgtggtt tatctcgacc tgcgtcacct
     1141 cggcgagaaa aaactgcatg aacgtctgcc gttcatctgc gaactggcga aagcgtacgt
     1201 tggcgtcgat ccggttaaag aaccgattcc ggtacgtccg accgcacact acaccatggg
     1261 cggtatcgaa accgatcaga actgtgaaac ccgcattaaa ggtctgttcg ccgtgggtga
     1321 atgttcctct gttggtctgc acggtgcaaa ccgtctgggt tctaactccc tggcggaact
     1381 ggtggtcttc ggccgtctgg ccggtgaaca agcgacagag cgtgcagcaa ctgccggtaa
     1441 tggcaacgaa gcggcaattg aagcgcaggc agctggcgtt gaacaacgtc tgaaagatct
     1501 ggttaaccag gatggcggcg aaaactgggc gaagatccgc gacgaaatgg gcctggcaat
     1561 ggaagaaggt tgcggtatct accgtacgcc ggaactgatg cagaaaacca tcgacaagct
     1621 ggcagagctg caggaacgct tcaagcgcgt gcgcatcacc gacacctcca gcgtgttcaa
     1681 caccgacctg ctctacacca ttgaactggg ccacggtctg aacgttgctg aatgtatggc
     1741 gcactccgca atggcacgta aagagtcccg cggcgcacac cagcgtctgg acgaaggttg
     1801 caccgagcgt gacgacgtca acttcctcaa acacaccctc gccttccgcg atgctgatgg
     1861 cacgactcgc ctggagtaca gcgacgtgaa gattactacg ctgccgccag ctaaacgcgt
     1921 ttacggtggc gaagcggatg cagccgataa ggcggaagca gccaataaga aggagaaggc
     1981 gaatggctga gatgaaaaac ctgaaaattg aggtggtgcg ctataacccg gaagtcgata
     2041 ccgcaccgca tagcgcattc tatgaagtgc cttatgacgc aactacctca ttactggatg
     2101 cgctgggcta catcaaagac aacctggcac cggacctgag ttaccgctgg tcctgccgta
     2161 tggcgatttg tggttcctgc ggcatgatgg ttaacaacgt gccaaaactg gcatgtaaaa
     2221 ccttcctgcg tgattacacc gacggtatga aggttgaagc gttagctaac ttcccgattg
     2281 aacgcgatct ggtggtcgat atgacccact tcatcgaaag tctggaagcg atcaaaccgt
     2341 acatcatcgg caactcccgc accgcggatc agggtactaa catccagacc ccggcgcaga
     2401 tggcaaagta tcaccagttc tccggttgca tcaactgtgg tctgtgctac gccgcgtgcc
     2461 cgcagtttgg cctgaaccca gagttcatcg gtccggctgc cattacgctg gcgcatcgtt
     2521 ataacgaaga tagccgcgac cacggtaaga aggagcgtat ggcgcagttg aacagccaga
     2581 acggcgtatg gagctgtact ttcgtgggct attgctccga agtctgcccg aaacacgtcg
     2641 atccggctgc ggccattcag cagggcaaag tagaaagttc gaaagacttt cttatcgcga
     2701 ccctgaaacc acgctaagga gtgcaacatg acgactaaac gtaaaccgta tgtacggcca
     2761 atgacgtcca cctggtggaa aaaattgccg ttttatcgct tttacatgct gcgcgaaggc
     2821 acggcggttc cggctgtgtg gttcagcatt gaactgattt tcgggctgtt tgccctgaaa
     2881 aatggcccgg aagcctgggc gggattcgtc gactttttac aaaacccggt tatcgtgatc
     2941 attaacctga tcactctggc ggcagctctg ctgcacacca aaacctggtt tgaactggca
     3001 ccgaaagcgg ccaatatcat tgtaaaagac gaaaaaatgg gaccagagcc aattatcaaa
     3061 agtctctggg cggtaactgt ggttgccacc atcgtaatcc tgtttgttgc cctgtactgg
     3121 taaggagcct gagatgatta atccaaatcc aaagcgttct gacgaaccgg tattctgggg
     3181 cctcttcggg gccggtggta tgtggagcgc aattattgcg ccggtgatga tcctgctggt
     3241 gggtattctg ctgccactgg ggctgtttcc gggtgatgcg ctgagctacg agcgcgttct
     3301 ggcgttcgcg cagagcttca ttggtcgcgt attcctgttc ctgatgatcg ttctgccgct
     3361 gtggtgtggt ttacaccgta tgcaccacgc gatgcacgat ctgaaaattc acgtgcctgc
     3421 gggcaaatgg gttttctacg gtctggctgc tatcctgaca gttgtcacgc tgattggtat
     3481 cgttacaatc taacgtatcg ccaatgtaaa tccggcccgc ctatggcggg ccgttttgta
     3541 tggaaaccag accttatgtt caaaacgacg ctctgcgcct tattaattac cgcctcttgc
     3601 tccacatttg ctgcccctca acaaatcaac gatattgtgc atcgcacaat taccccgctt
     3661 atagagcaac aaaagatccc cggtatggcg gtggcggtaa tttatcaggg taaaccttat
     3721 tactttacct ggggctatgc ggacatcgcc aaaaagcagc ccgtcacaca gcaaacgttg
     3781 tttgagttag gttcggtcag caaaacattt acgggcgtgc ttggtggcga cgctattgct
     3841 cgaggggaaa tcaagttaag cgatcccaca acaaaatact ggcctgaact taccgctaaa
     3901 cagtggaatg ggatcacact attacatctc gcaacctaca ctgctggcgg cctgccattg
     3961 caggtgccgg atgaggtgaa atcctcaagc gacttgctgc gcttctatca aaactggcag
     4021 cctgcatggg cgccaggaac acaacgtctg tatgccaact ccagtatcgg tttgttcggc
     4081 gcactggctg tgaagccgtc tggtttgagt tttgagcagg cgatgcaaac acgtgtcttc
     4141 cagccactca aactcaacca tacgtggatt aatgtaccgc ccgcagaaga aaagaattac
     4201 gcctggggat atcgcgaagg caaggcagtt catgtttcgc caggggcgtt agatgctgaa
     4261 acttatggtg tgaagtcgac cattgaagat atggcctgct gggtacgaag caatatgaat
     4321 ccccgtgata tcaacgacaa aacacttcag caagggatac aactggcaca atctcgctac
     4381 tggcaaaccg gcgatatgta tcagggcctg ggctgggaaa tgctggactg gccggtaaat
     4441 cctgacagca tcattaacgg cagtggcaat aaaattgcac tggcagcaca ccccgtaaaa
     4501 gcgattacgc ccccaactcc tgcagtacgc gcatcatggg tacataaaac aggggcaacc
     4561 ggcggatttg gtagctatgt cgcgtttatt ccagaaaaag agctgggtat agtgatgctg
     4621 gctaacaaaa actatcccaa tccagcgaga gtcgccgccg cctggcagat tctcaacgct
     4681 ctacagtaaa atttcatcgg gtccgaattt tcggaccttt tctgcgcttt tccttgctgt
     4741 catctacact tagaaaaaaa ccagtaagga aacatttatg cgcctgctcc ctctcgttgc
     4801 cgcagcgaca gctgcatttc tggtcgttgc ctgcagttct cctacgccgc cgcgtggcgt
     4861 aacagtagtg aataatttcg acgccaaacg ttatctcggt acctggtatg agattgcccg
     4921 ttttgatcac cgttttgaac gtggactgga aaaagtcacc gcaacataca gcctgcgtga
     4981 tgacggcggc ctgaatgtca ttaataaagg ctataaccct gacagaggaa tgtggcagca
     5041 gagtgaaggg aaagcgtact ttaccggcgc accaactcgc gctgcgctga aagtgtcatt
     5101 ctttggtcct ttctatggcg gttataacgt tattgcactc gatcgggaat accgccatgc
     5161 gctggtttgc ggcccggacc gcgactacct gtggatactc tcccgcacgc caaccatttc
     5221 tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg gaagggtttg atgtcagtaa
     5281 gtttatttgg gtacagcagc ccggtagtta gtgagtgctg agtttcagac caataatccc
     5341 caatacgatt aacgccagac tcgccaggcg catcgggtta gcggactcac cgagcagcac
     5401 aatgccggtg atggccgcgc cgactgcgcc aatacccgtc cacacggcat aagccgtccc
     5461 tactggtaac gatttcatcg cccaggcaag tagcgccata ctgacaatca tcgccgtcac
     5521 ggtaataaca ctcggcgtca aacgactaaa gccgtgggta tatttcaggc caacggccca
     5581 taccacttcc agcagaccag caataactaa gataatccag gacatatcag gctccggaga
     5641 attggggccg tccccggtga aaagaggcgt ttgcaggtcg tcctgcaaag ctgatggttg
     5701 aacggatatt ttgccaggtg gcaggggaat ttcaactatt accttagtga ctatcgcaga
     5761 aaaaacaaac ggcacgacat acctgtcgta ccgttattgc ttattgctat tgctgcgctt
     5821 tcgttgctgc gccagaaatc gcgttaccgc catcagaaat gtcttcacca acgccacgcg
     5881 tggtgttgca ggcagttaat actgttgaaa gcaccagaac agaaaagatc gctgcaattg
     5941 tcttcttcac cataacgtct tccttttagg taatgttatt tatgtttgcc tatagcaaat
     6001 taataataga caacatcgcc aaaaatgacg gaaatctgat gatttttcgg aagagaagaa
     6061 aaattagctg gcagcgcgag agatggagtt gccgagatgt ttgatgtctt cgccgaaacc
     6121 gcgagcggtg ttacagcccg tgagcagcgt gctggcaagc aaaacaagaa cgataaggcg
     6181 tttcatcatc tctgacctga ataagtgatg gtgcagcctg caggccgcac cacaaccgca
     6241 ttacttcacg cgagagacgt attcaccaga gcgggtatcc actttgatga cttcgccgat
     6301 ttgtacgaac agcggaacct taaccacagc gccagtagac agggtagccg gtttgccgcc
     6361 agtacctgcg gtatcacctt tcaggcccgg atcggtatca acgatttcca gttcaacgaa
     6421 gttcggcgga gtaacggaga tcggctgacc attccacaga gttacgatac actctgcctg
     6481 atccagcagc catttagcgt tgtcgccaat tgcttttgca tcagcagaca gctgctcgaa
     6541 agtttcgttg ttcatgaagt gccagaactc accgtcgttg tacaggtaag tcaggttcat
     6601 atcgacaaca tcagcgcctt cagcggaatc agtagatttg aaggtttttt ctacgcgagt
     6661 accggtcagc agacgacgca gtttaacgcg agcaaatgcc tggcctttac ccggttttac
     6721 gaattcactc gcttcaaccg cgtaaggttc gccgtctaac atgattttaa gaccagcacg
     6781 aaaatcgttg ctatagtacg ttgccataag gccctctgaa atttgttaat tggtagctaa
     6841 gccacaaaat ggcgcatatt gtaaccctaa ataccccatc cagagaagat tggttaacgc
     6901 aacttgccga tgttgtgacc gatcctgatg aacttctgcg tcttttgaat atagacgcgg
     6961 acgaaaaact gttggccgga cgcagcgcca aaaagctgtt tgccctgcgt gtgccccgct
     7021 catttatcga tcgcatggag aaaggcaatc cggacgatcc tcttttgcgt caggtactta
     7081 cctcgcaaga tgagtttgtc gtcgcgtccg gattctccac cgacccgctg gaagaacagc
     7141 acagcgtagt gcctggtttg ttgcataaat accacaaccg ggcgcttttg ctggtcaaag
     7201 gcggctgcgc ggtaaattgc cgctattgct tccgtcgcca cttcccctat gccgaaaatc
     7261 agggcaacaa gcgtaactgg cagactgctc ttgagtatgt tgctgcgcat ccggaactgg
     7321 acgagatgat tttctccggc ggcgatccgc tgatggcgaa agatcacgag ctggactggt
     7381 tgctcacaca actggaagcc atcccgcata tcaaacgtct gcggattcac agccgtctgc
     7441 cgattgtgat cccggcacgt atcaccgagg cgctggttga acgctttgcc cgttctacgc
     7501 tgcaaatctt gctggtgaat cacatcaatc atgccaatga ggtagatgaa acgttccgtc
     7561 aggcgatggc taaattgcgc cgtgtcggtg tcaccctgct taaccagagc gttctgttac
     7621 gtggtgtgaa cgataacgca caaacgctgg caaacctgag taatgcgttg ttcgatgccg
     7681 gcgtaatgcc gtattacctg catgtgctcg ataaagtaca gggcgcggcg cattttatgg
     7741 tgagtgatga cgaagcacgg cagattatgc gtgagttgct gacactggtg tcgggttatc
     7801 tggtgccgaa actggcgcga gaaatcggcg gcgaacccag caaaacgccg ctggatctcc
     7861 agctacgcca gcagtaactg attaaacagg gaagttaaac attagcctga tgcgctacgc
     7921 ttatcaggcc tacattgtgc attgaaattg aatggatttg caatgaactg taggccggat
     7981 aaggcgttta cgccgcatcc ggcaagagca acaaacgcaa gtacagcaac ctgtcattaa
     8041 actgggaaat ttaatctttg cccgttgcat aacctttaat tattcatata cattgctgtt
     8101 gttactcttt atatagccaa caccaataat taacctttct tttctgttta tatagagtga
     8161 gtacggctat ttatgttatt ttaacaacta ttactaccaa gcccatttat ctggcttata
     8221 aattaaaatt aattatttaa ttgagaaatg gttagggaga acctacatgg ccataagcat
     8281 caagggtgtt aataccggtg ttattcgcaa gagtaataat ttcattgcac tggcgctgaa
     8341 aattaaagaa cctcgcaata aagaatcgtt gttctttatg tctgtcatgg aactgcgcga
     8401 cctgctgatt gcgctggaaa gtcgtatgca tcaaaagcat aaactggatg ctgctgcacg
     8461 ccttcagtat gagcaagctc gcgataaagt cattaagaaa atggcagaaa acatcccaga
     8521 aattctggtt gatgagctta aaaatgccga tattaatcgt cgggttaaca cgctggaact
     8581 taccgataat caaggcgaaa atcttacctt cgtattaacg ctacatgacg gtagcaaatg
     8641 tgagttagtc gtcaatgagt tgcagattga aatgctggcg cgagcaatca ttcatgcaat
     8701 taataatgcg gaaatgcgtg aactggcatt acgtattacc tcactgttag atttcctgcc
     8761 gttatatgac gttgactgcc aggaaaatgg caatctggaa tatgacacct attcccaacc
     8821 ggaatggaaa cataacctgt tcgaccatta tctggcggtg ctttaccgct ttaaagatga
     8881 aagtggcaaa gaacaattta gcggcgcagt ggtgaaaacg cgtgaagcaa cgccgggtaa
     8941 agaaatagag gcgatcactc gccgaatgct tgatttcagc caacgactga aaaaactcgc
     9001 gggtgtgcca tgtcaggtct atgttcgcac cgtagcggcg aataacgccc aaccgctaac
     9061 ccaggatcaa tgcttacgcg cgctgcatca cttacgtgtt caatccacca gcaaaactgc
     9121 accacaggcg aaataatctc atctttgcta atgcgaggct ggagccatcc agcctgaaat
     9181 gactgataac gtgatcgttg gtcatgccgg atgcggcgta aacgccttat ccggcctaca
     9241 aattctcaag cattcaatca gttgcaatgc ctg
//
LOCUS       DAZGJW010000169         9192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZGJW010000169 DAZGJW010000000
VERSION     DAZGJW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.169"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..96)
                     /gene="ldrC"
                     /locus_tag="ACHKIK_004292"
     CDS             complement(<1..96)
                     /gene="ldrC"
                     /locus_tag="ACHKIK_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO9085344.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVSWWR"
     gene            complement(501..1601)
                     /gene="chaA"
                     /locus_tag="ACHKIK_004293"
     CDS             complement(501..1601)
                     /gene="chaA"
                     /locus_tag="ACHKIK_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO9085345.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1871..2101
                     /gene="chaB"
                     /locus_tag="ACHKIK_004294"
     CDS             1871..2101
                     /gene="chaB"
                     /locus_tag="ACHKIK_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO9085346.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            2259..2954
                     /gene="chaC"
                     /locus_tag="ACHKIK_004295"
     CDS             2259..2954
                     /gene="chaC"
                     /locus_tag="ACHKIK_004295"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO9085347.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2998..3351)
                     /gene="ychN"
                     /locus_tag="ACHKIK_004296"
     CDS             complement(2998..3351)
                     /gene="ychN"
                     /locus_tag="ACHKIK_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO9085348.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3536..4930
                     /gene="ychO"
                     /locus_tag="ACHKIK_004297"
                     /gene_synonym="iatA"
     CDS             3536..4930
                     /gene="ychO"
                     /locus_tag="ACHKIK_004297"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO9085349.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4931..5581)
                     /gene="narL"
                     /locus_tag="ACHKIK_004298"
     CDS             complement(4931..5581)
                     /gene="narL"
                     /locus_tag="ACHKIK_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO9085350.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5574..7370)
                     /gene="narX"
                     /locus_tag="ACHKIK_004299"
     CDS             complement(5574..7370)
                     /gene="narX"
                     /locus_tag="ACHKIK_004299"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO9085351.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7709..9100
                     /gene="narK"
                     /locus_tag="ACHKIK_004300"
     CDS             7709..9100
                     /gene="narK"
                     /locus_tag="ACHKIK_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO9085352.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         2234 a         2252 c         2368 g         2338 t
ORIGIN      
        1 acgccaccag ctgacaatcg ctgcggtaat aattcccgcc aggatcggtg ctgccaggtc
       61 gtgccagaaa atcatggcaa actgcgcgag cgtcatatag ccgccttgtt gtaatgacaa
      121 cattttgcgg ctattcttga agtgtctggt ttcaagatta gcccccgttt tgttgtcagg
      181 ttttacctct caacgtgcgg gggttttctc tttccagcaa ccaatgccac cagggataaa
      241 gcccccgcaa cattgcgcct caccggataa cgccggcttg gtgtggatac tacgtcgcaa
      301 ttcatcttca cttcatccct gaaatgtttg caataaagag tacattccgg cttttcaaca
      361 gctgttgcag tggtttcgtg caggttgtgg tacaggctcg caattctgat taacgagctg
      421 tccaggtagt atgaagaaat acaataagca ggaatttatc ttatctcccc cgatttccgt
      481 catcggagga gatataactg tcaggcaaat atcgtcatca aataggcggc aaacagtgcc
      541 agatgcgctg cgccattgag cacgttagta cgtccggtgg agaaggagat atggcacagc
      601 actaaagagg ccaccatcac caccatttct ggcgcaccaa gtgcaaactg taattcgtta
      661 cccgtcataa aggcaattag cgtgacgaca ggtacggtaa gcgaaatggt tgctaacact
      721 gaaccaaaga acagattcat cgcgcgctgg acctggttgt tcaacactgc ttttaacgca
      781 cctaaacctt ccggcgacag aatcaacaga gccaccagga agccagtaaa ggcgacaggg
      841 gcattcatgc tgtcgagcaa tgtctccagc gggctggcat tcattttggt gactgcaata
      901 acggcaatca gatggataat caaccagata gcatgccaca cgctgctatg ggcagacggt
      961 ttaccgtgat gcgggtcatc gtcatcactg tcatcttcgt gctcgtagac aaacaaactt
     1021 tgatgcgttt tggtctggat cagcaaaaat acgccataca ttgccgcaga aattaatgct
     1081 accagtaacg cctgaccggt tgaaaaattc gccgcaggca gagccattgg aaataccagt
     1141 acgattatcg ccagggggaa cagcgcaatt aaatactgct tgataccaaa cagattcata
     1201 tattgggtgg caaacttacg accgcccaac aataatgaaa agccaaccag cccaccggta
     1261 acaatcataa tgattgaata gagcgtatca cgcattagcg ttggcgcggc gtcgccggtt
     1321 gccattaaag ctgaaatcaa actgacttca agaataacca ctgaaaggct aagaataagc
     1381 gaaccgtaag gttctcccag gcgatgggct aatacgtccg catgacggac aacactaaag
     1441 gcgctactta aaataccaat aagcgcaaga agattgatgg caatgaccac tggtagtgtc
     1501 tggctgcttc cccacaggaa cagcactacc agcgccagaa ccgggaaaat aagcgaagtc
     1561 tccttgtggc gggtttttac cgcctcttga gcatttgaca ttatggttat ccctttgcag
     1621 atgaatttat cgaaaatgta aaaaataggt aggaaaaata acagaaagtg tctggatatc
     1681 ggtaacattt tacgaatttt taccccactg tcgatttttt actcattggg acataaaaat
     1741 aagtattacg tttagacaat gtttgtttta gcgtttctta agaacagtct gacatgaaaa
     1801 ttcttatgtt ttggcaagag tagatattgt tgaccacact taatgttcaa ctttgtaaaa
     1861 ggagtcaacg atgccgtata aaacgaaaag cgatctgccg gaaagcgtaa agcacgttct
     1921 accgtctcat gcccaggata tctataaaga agcgttcaac agcgcatggg atcaatataa
     1981 agataaagac gatcggcgtg atgacgccag tcgcgaagaa accgcgcata aagtggcctg
     2041 ggctgctgtg aagcatgaat atgccaaagg ggatgatgat aaatggcata aaaaatcgta
     2101 aaaccggtag cttagttaaa gctattcgtg cggtgttgcc ttgcaagtgg tccgtggatt
     2161 gcatattgtc ccgttagtgg tttcaaaatg agcagtaaaa atgtccggaa gacaccaaaa
     2221 agttgtcgca gggaagtatg cagtggcgga ggtgtaaggt gataacgcgt gatttcttga
     2281 tgaatgccga ttgtaaaacg gcatttggtg ccattgaaga atcactctta tggtcagcag
     2341 agcaacgggc ggcttcgctg gcggcgacgc tggcttgtcg acctgatgag ggaccggtgt
     2401 ggatcttcgg ttatggatcg ttgatgtgga atccggcact ggagtttacc gagtcgtgca
     2461 ccggtacact ggttggatgg catcgcgcgt tttgcctgcg cctgaccgcc gggcgcggga
     2521 ctgcgcacca gccgggacgg atgcttgcac tgaaagaggg cggacgcact acaggcgtcg
     2581 cctatcgact gccagaagag acgctggagc aggaactaac cctgttgtgg aagcgcgaga
     2641 tgattaccgg ctgttatctg ccaacctggt gtcagcttga tcttgatgat ggatgcacag
     2701 taaacgccat tgtgtttatt atggacccgc gacatccaga atatgaatct gacactcgcg
     2761 ctcaggtcat cgcgccgttg attgcggcgg cgagcggtcc gctgggaacc aacgcacaat
     2821 acctgttttc actggaacag gagcttatca aactgggaat gcaggacgat gggctgaatg
     2881 atttgctggt atcggtaaaa aaactgctgg cggagaatta tccggatggt gtgttacggc
     2941 cgggattcgc ctgagtaaac ttccctcata gtggggcgtc agacgccccc tcaaacatta
     3001 aaatgtgagc actttatcgg ctgacagcgt ccattgcgcc agttccacaa gagtaccgat
     3061 ttccacccca tcaatcagag gaagtgtact aatcccgcgc ccgtcggtac aggttttgca
     3121 caatttcacc ggtacattct gagcggtaag gatctccagc atttgctgaa tgttgtagcc
     3181 ttcccctggt ttttgcccgc gcaacccggc tgtgaccgca tcagacatca ggaacagacg
     3241 cagatccaga ttgctctcct gctctcgtaa cgcaatggct agccgcaagc tgttaaacaa
     3301 ggattcgctc ccgtaaggtg cgccattggc aacgatcacg attttttgca ttatttactc
     3361 ctgtattcag ggaattagac attcatcttc tatcttactg cttctgcagc gtctgaccaa
     3421 tcggtcacat ttttaaggat tttcctgaaa gcgcgagaaa atacgacaaa agttgccagt
     3481 aatcgttatt ctttaaggct atggtttttc attattaccg gaagttaccg acgttttgag
     3541 ccgtttcgtt cctcgcatta tcccgtttta tttactcttg cttgtagcag gcggtacagc
     3601 taacgcacaa tctaccttcg agcaaaaagc ggcaaatccc tttgataata acaatgatgg
     3661 tctgccggat ttaggcatgg ctcccgaaaa tcatgatggg gaaaaacact ttgctgaaat
     3721 tgtgaaagat ttcggcgaaa ccagtatgaa tgataacggg ctggatactg gcgagcaggc
     3781 aaaagctttc gcattgggaa aagtccgcga cgcgcttagt caacaggtta atcagcacgt
     3841 agagtcctgg ctatcaccgt ggggaaatgc cagtgttgac gtcaaagtgg ataacgaagg
     3901 acatttcacc ggcagtcgtg gaagctggtt tgtgccgtta caagataatg atcgttatct
     3961 cacctggagc cagcttggtc ttactcagca ggatgatggg ttggtgagca atgtgggcgt
     4021 tgggcaacgc tgggcgcgcg gcaactggct ggtgggttat aacacttttt atgacaactt
     4081 gctggacgaa aatcttcagc gagcgggctt tggtgccgaa gcgtggggcg aatatttgcg
     4141 actatcggca aacttttatc agccgtttgc tgcatggcat gaacagacag ccacgcagga
     4201 acaacggatg gcgcgcgggt atgacctgac agcccggatg cgcatgccgt tctatcaaca
     4261 cctcaatacc agtgtcagcg tagaacagta ttttggtgat cgtgttgatt tgtttaactc
     4321 tggtacgggt tatcacaatc ccgtcgcgtt gagtctggga ttaaattaca cccctgtgcc
     4381 attagtcact gtgacggccc agcataaaca gggtgaaagt ggcgagaatc aaaataacct
     4441 cgggctgaat cttaattacc gctttggtgt accgctcaaa aaacaacttt ctgcgggtga
     4501 ggttgccgaa agtcagtcgt tacgtggtag tcgctatgac aatccgcagc gaaataatct
     4561 accgactctt gagtaccgac agcgaaaaac gttaacggtg tttctggcga caccgccgtg
     4621 ggatctaaaa cctggcgaaa cagtgccgct gaaattacaa atccgcagtc gttacggtat
     4681 tcggcaactg atttggcagg gcgatacgca gatattaagt ttgacgccgg gcgcacaagc
     4741 caacagcgcg gagggctgga cgctgatcat gcctgactgg cagaacgggg aaggggcaag
     4801 caatcactgg cgattgtcgg tggtggtgga agataaccag gggcagcgtg tctcctccaa
     4861 tgagatcacg ctaacgcttg tcgaaccgtt cgatgcattg tcaaacgacg aactgcgctg
     4921 ggaaccgtaa tcagaaaatg cgctcctgat gcacccatac cgctgcttcc acgcgagact
     4981 tgagcttcat tttcttcagc atgtgcttga cgtgcacttt tactgtgctt tcggtgatat
     5041 ccaggcggcg ggcaatcatc ttgttcggca aaccctgggc aatcagcttg agaatatcgc
     5101 gctcgcgtgg ggttaactgg ttaacatcgc gctcagtagt ggcacggtta gcgcgcaagc
     5161 tggcggccag aacaggcgtt aatgcttcgc ttaataccat ttcgccagca gctgcctgat
     5221 gcaatgcttt cagcagatct tccggttcca tatcttttaa cagataacca tccgcgccgc
     5281 gtttcagtgc ggtgaccaca tcttcttcat ggttagagac gctgaatacc acaatgcgcc
     5341 ctgagaggga cttttcgcgc agtttatcca gcgtttccag accgttcatg ccgggcatat
     5401 tgagatctaa caggatcaga tcgggatcaa gagactccgc cagttcaata ccctgttcgc
     5461 cattactcgc ttcgccaacc acggtgatat ctggtgccat actgataagc tgttttacgc
     5521 cagttcgcag catcgggtga tcgtcaatca gcaggatagt agccggttcc tgattactca
     5581 tgggtatctc cttggacgtc tgtgaaagtt ttttcgggaa taaaggtgac caccacttcg
     5641 gtgccacctg attcacgacg gcggacgcgg caatcgcctc gtaaactttg cgcacggtcg
     5701 cgcattatta tcatgccgta gtgattgctg cggatggcat tttcaggcac gccgcagccg
     5761 ttatcctgga cggtcagttt gacctgatta tcgttttgcg ccaccgtcac cacgacttca
     5821 ctcgcttgcg agtgtttgag ggcgttactt aatgcctcac gggcaatttg caacaagtgg
     5881 attgcctgat gcgaaggcac caggcgaggc ggcaattgat aatccagctt caccgggaag
     5941 ccaaatttgg cgctgtactc ttcgcaactc gcctccagcg ccggacgtaa tccaggctcg
     6001 gtgagctgca agcggaatgt ggtgagcaat tcacgcaact gcgcccagga tgcattcagt
     6061 tcgttacgga tctgacttaa cagttcgcgg ctgctttctg gcagcgcatc gccctgcatc
     6121 tgtaaacaac tcacctgcat cttcatgcaa gagagagatt gggcaataga atcatgcagt
     6181 tcgcgcgcaa tggtggcacg ctcttccatc acgatcaact gttgctgacg ttcctgatgg
     6241 cgatccagcg ccagcgtggc ggtgagttgt tcaaccaggg tatccaccag ttgttgttga
     6301 tcatggctaa gatgacgccc ctgcggcagg gtcgccagca aaataccgta ctgcgtatga
     6361 gagtcagcca gccgccactt cagggttgtg ccgcgatcac caacgggtaa tacgccgcgc
     6421 gggcagagct ggcagccttt atcatcacaa gtcatatctg gttggcaggt aaactcctga
     6481 tgattctctt catcatcagt atcataaacc cgcagttcga tatcacgtag cagggttaaa
     6541 ttctgtaagc cgttgagtac aggtgacagg cgttcacaca gcggggcttg ggaatgcaaa
     6601 cggcggttag cctgccataa aaaagagagg atctgatttt tatgctccag cccggcggtt
     6661 ttctcctgaa cccgctgctc aagtacggca taactttcgg ccagttctgc agacatattg
     6721 ttcaacgcag ttccaagcat cgccatttcg ttgcgcccgc tgatgtttgc gcgttgggta
     6781 aaatcgcgat gactgacggc actcgccatt gccagcagtt gccgccacgg ttgtagcagt
     6841 cgcgcccgca accagataat agtgaacacc agtaaaagtg ccataaatac cgccattacc
     6901 cgatggacca gtaccactgt ctcgatgcgc atttccgtgg tgcggtcaaa accagatacc
     6961 agttgatcaa gcccggcaac aaactggctg acatccgctg acaccgtttc tcggttttgt
     7021 gcacgcatca acgcagggat cagttcatta cgccagtaat cttgtaaacc ctgtaattgc
     7081 gccagttgtc cgtctcgttc tgctgctcga gtcaactcgg cgctaaatgc cgtttgttcc
     7141 atctctttaa ttaagggctt gtctttctcg cttaatggca ctgctgccaa cagacggtaa
     7201 ctttgcatgc gcagcgatcc cgctttgttg atcgcatggg cgctgccctg aacgccttgc
     7261 accagccagc cagaaaccgc catccctgcc agtccaatag cagtagaaag caacacaata
     7321 agcgcaacct gattaaccag ggtgagcgga gagagacaac gtttaagcat gtaaacctct
     7381 tccttcaggc tttaaatgag caataacctt aatgaatgtg acgatacatt ctggaatggc
     7441 agtattctcg gctattggct gaagtatacc catacctgga aagagttact ccttatttgc
     7501 cgtgtggtta gtcgctttac atcgttaagg gtagggattc tacagcaccg tgaaaaatct
     7561 cataattttt atgaaatcac tgtactcact atgggtaatg ataaatatca atgatagata
     7621 aagttatctt atcgtttgat ttacatcaaa ttgcctttag ctacagacac taaggtggca
     7681 gacatcgaaa cgagtatcag aggtgtctat gagtcactca tccgcccccg aaagggctac
     7741 tggagctgtc attacagatt ggcgaccgga agatcctgcg ttctggcaac aacgcggtca
     7801 acgtattgcc agccgcaacc tgtggatttc cgttccctgt ctgctgctgg cgttttgcgt
     7861 atggatgttg ttcagcgctg ttgcggtgaa cctaccgaaa gtcggcttta attttacgac
     7921 cgatcagcta tttatgttga ctgcgctgcc ttcggtttct ggcgcgttat tacgtgttcc
     7981 atactccttt atggttccta tcttcggtgg tcgtcgctgg acggcgttca gcaccggtat
     8041 tctgattatt ccttgcgtct ggctgggttt tgccgtgcag gatacctcca cgccttatag
     8101 cgtcttcatc atcatctctc tgctatgcgg ctttgctggc gcgaacttcg cctccagtat
     8161 ggcaaacatc agcttcttct ttccgaaaca gaagcagggt ggcgcgctgg gtctgaatgg
     8221 tggtctgggc aacatgggcg tcagcgtcat gcagttggtt gctccgctgg tggtatcact
     8281 gtcgattttc gcagtatttg gtagccaggg cgtcaaacag ccggatggga ctgagctgta
     8341 tctggcgaat gcgtcctgga tatgggtgcc gttccttgcc atcttcacta ttgcggcgtg
     8401 gtttggcatg aacgatcttg ctacctcgaa agcctccatc aaggagcagt tgccggtact
     8461 caaacggggt catctgtgga ttatgagcct gctgtatctg gcaaccttcg gctccttcat
     8521 cggcttctcc gcgggctttg caatgctgtc aaaaacgcag ttcccggatg ttcagattct
     8581 gcaatacgct ttcttcgggc cgtttattgg tgcgctggcg cgttctgcag gtggtgcatt
     8641 atctgaccgt ctgggcggaa ctcgtgtcac gctggtgaac tttattctga tggcgatttt
     8701 cagcggcctg ctgttcctga ccttaccgac tgacgggcag ggcggaagct tcatggcgtt
     8761 cttcgcggtc ttcctggcgc tgttcctgac agctgggctg ggtagtggtt ccactttcca
     8821 gatgatttca gtgatcttcc gtaaactgac aatggatcgc gtgaaagcag aagggggttc
     8881 tgacgaacgt gcgatgcgtg aagcggcaac cgacacggca gcggcgctgg gtttcatctc
     8941 tgcgattggc gctattggtg gcttctttat cccgaaagcg tttggtagct cgctggcatt
     9001 aacgggttcg ccagtcggcg caatgaaggt atttttgatt ttctatatcg cctgcgtagt
     9061 gattacctgg gcggtatatg gtcggcattc taaaaaataa accgttactc gtcatacttc
     9121 gggttacatg tgctgcggct gcgttcattc accccagtca cttactttag taagctcctg
     9181 ggattcattc ac
//
LOCUS       DAZGJW010000170         9125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZGJW010000170 DAZGJW010000000
VERSION     DAZGJW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.170"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..63)
                     /locus_tag="ACHKIK_004301"
     CDS             complement(<1..63)
                     /locus_tag="ACHKIK_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085353.1"
                     /translation="MSQNLDATAINQIHALISAQG"
     gene            complement(129..491)
                     /gene="yfdP"
                     /locus_tag="ACHKIK_004302"
     CDS             complement(129..491)
                     /gene="yfdP"
                     /locus_tag="ACHKIK_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO9085354.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            complement(1158..1832)
                     /gene="ymfK"
                     /locus_tag="ACHKIK_004303"
     CDS             complement(1158..1832)
                     /gene="ymfK"
                     /locus_tag="ACHKIK_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HFO9085355.1"
                     /translation="MKTVHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            1923..2123
                     /gene="ymfT"
                     /locus_tag="ACHKIK_004304"
     CDS             1923..2123
                     /gene="ymfT"
                     /locus_tag="ACHKIK_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO9085356.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            2167..2718
                     /gene="ymfL"
                     /locus_tag="ACHKIK_004305"
     CDS             2167..2718
                     /gene="ymfL"
                     /locus_tag="ACHKIK_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO9085357.1"
                     /translation="MGKYHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAAGTHYIADAVAQSAGGVFVSLPEIEEVENADINQR
                     LLEVIEQIGNYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            2864..3043
                     /locus_tag="ACHKIK_004306"
     CDS             2864..3043
                     /locus_tag="ACHKIK_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9085358.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAH"
     gene            2973..3866
                     /locus_tag="ACHKIK_004307"
     CDS             2973..3866
                     /locus_tag="ACHKIK_004307"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO9085359.1"
                     /translation="MSALVLCGNSGVILLSVKDHQHIDSAIPGRYTVQAPYKAGAGRG
                     NPEFTKAHNRALAVFLCHEQHYAQIMVGRAGPTSVGPGSLVTGISTPVRLTTNKVVES
                     LGGELLKITKEAAIMATIPTLTQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRTL
                     ECSASFTARNFSVSDYTDCTGRKLPCYQITRDGFAFLAMGFTGKRAAQFKEAYINAFN
                     QMEKQLSKLAVPSDVAHNASVLYSYISSIHQVWLQQLYPMLEKAESPLAVSLYDRIND
                     AAFLARLIHSSLNSSEVRGRK"
     gene            3863..4087
                     /locus_tag="ACHKIK_004308"
     CDS             3863..4087
                     /locus_tag="ACHKIK_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085360.1"
                     /translation="MIRNIFKRFTNQTFRCPRPGQWYTTPAGHVLRVSLVDRECQKVI
                     CEPLGRNYRVSMPLIAFRSGKNMKHLGGAA"
     gene            4084..4902
                     /locus_tag="ACHKIK_004309"
     CDS             4084..4902
                     /locus_tag="ACHKIK_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9085361.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSLGGSANNSL
                     SGAANSPGSAGVAPGGSAGDSPRTSHSFEPVKEPVNEPIAVGASADESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVNPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSAARRDDPYFKASYDNVDYSQIPAGFRG"
     gene            4899..5393
                     /gene="yfdN"
                     /locus_tag="ACHKIK_004310"
     CDS             4899..5393
                     /gene="yfdN"
                     /locus_tag="ACHKIK_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO9085362.1"
                     /translation="MIMSLLNDVQKFIEAHPGCASGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRQTEVLQESEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            5393..6046
                     /locus_tag="ACHKIK_004311"
     CDS             5393..6046
                     /locus_tag="ACHKIK_004311"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO9085363.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASSMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            6043..6369
                     /locus_tag="ACHKIK_004312"
     CDS             6043..6369
                     /locus_tag="ACHKIK_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO9085364.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENARLRATHWLH
                     ERGLKV"
     gene            6366..6755
                     /locus_tag="ACHKIK_004313"
     CDS             6366..6755
                     /locus_tag="ACHKIK_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9085365.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISEAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKPAGAAA"
     gene            6775..7584
                     /locus_tag="ACHKIK_004314"
     CDS             6775..7584
                     /locus_tag="ACHKIK_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO9085366.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGGEQKKRPKYWLSNK
                     QTCELIEQLFTEGGIPPLEKNQPVSVINGGNNQGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSASEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            7592..8581
                     /locus_tag="ACHKIK_004315"
     CDS             7592..8581
                     /locus_tag="ACHKIK_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9085367.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKVLRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MATKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            8599..8940
                     /locus_tag="ACHKIK_004316"
     CDS             8599..8940
                     /locus_tag="ACHKIK_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO9085368.1"
                     /translation="MRDMYEVLDRWGAWAAADNSGVDWQPVAAGFKGLLPHGKKTRQQ
                     CDDDEGIMIDSCVARLRKYKPEEYELVIAHFVIGISLRTIAKKQKCSDGTIRKELQTA
                     LGFLDGVLSML"
BASE COUNT         2262 a         2129 c         2455 g         2279 t
ORIGIN      
        1 accctgagca gaaataaggg catggatttg attaattgcg gttgcgtcta agttctgaga
       61 cataataagt cctcactata taaagatatt cagtgatgag ataaataatc agttaattaa
      121 gaacgatatt aatgacctgc tgcgcggagt tttccgtcag gttcaccggc aagagtcagt
      181 aattgtccct ggtcttcctg cagaatagtc agtcgaccac cgcgattgac atacatcggc
      241 gtttcggtgg tgtcttcttc ggaaattttc ccgcggttag tcgggcgaac atatgagagt
      301 ttgtgtttga ttttcacacg gttctcatca aacggttcga tttccaggtt gagcgagacc
      361 ttacctttgg ttttcgtgtt catcacaccg gaagcgactt cactgagaac tgcgccgatt
      421 ttggtttcaa atacgccgcc gtccagctcc ccgataaatg cctgcacatc agtactgcgt
      481 tcgctagcca ttttgctgct cctcatcata tcgaccctgc aaggtcggtt ggtttctcca
      541 caaaacagag aagaacacct gcggtggcag ccgcccggct ggattgggtt atgagcccgt
      601 cgtccggtga tgctcttctc tgttttgtaa aaagagcggt accagccgga agcaagtgta
      661 caaactggta ccgccaaagc agtggctgtt gtggtggggt tgtcactcag gcgtatggtc
      721 aacctgacaa tccggtgtcc tcaacgggga aagagtaacc ccgccatact taccgccgcg
      781 ccatttcgcg gattaccaca acgctgagag cacttagcaa gttacggcac cacactttgt
      841 cgcggttcca taaatgccct catcgttgca ccctggtctc ttcccaggcg tcaaaccgga
      901 tcgccgcgct ggttaggcgt cttatcagca tcatcattga cttgcacatt ccggctacct
      961 ggtttttttg cccgagcaag gagtggattg tcccctttaa cgtccccaga ccgctaacga
     1021 cgcatgtgcc atacgccgtg ttacaaccaa attttgttta atcttgcctg cggcatgttt
     1081 cttttagata cattatgtat ctcaagggta cattgtcaag tataaaaaaa cctgccgaag
     1141 caggttataa atattgatta ggcctttatt ttgtatcttc ttggttttcc tgagaaaatt
     1201 actgtaccaa ttatagagca attaccgtta atcttaatgt aaggctcagg ccagtttggg
     1261 tttaatgctt tgagataacg ctgtgttcca tcttctatca atcgcttgaa ggtggtttcg
     1321 cctgtatcgt gcatcaatgc aataacgtcg tcaccgtggc aggcagggac ttcgggatca
     1381 acaaaaatca tgtctcccgg gcggtactca tcaatcattg aatcaccaat cacccgcaag
     1441 atataagtca tttcgccaca gggtacaggg cagggataag tttctgctgt gctcaaatca
     1501 acctcagaat agccaacttc tttccatgct ccggcctgta cccatgatat gacagggact
     1561 aacgttattt gtttgttagt gattgaaaca tcaggttttt ttgtgatgtt cgtggtctgg
     1621 tgttcttgat caagccatcc gacaggcagg tcgaaacatt tttcgatgtg ccgtgccatg
     1681 ctgtcaccga tatttttagt agcaccatct cccataaacc tgctggtctg ggttggctcg
     1741 cgatcaatca tggtggcaaa ggaagaattc ccgccaacac catctctcag ttttctggcg
     1801 ttagaccgcc ggatgtcatg gactgttttc ataaagaaat taaaaccttt gtaccgataa
     1861 ggtacaagta tcttgaaggt tcatattaat catgtaatat gtatattgga ggtacatatt
     1921 gtatgaaagc gtattgggac tctttaacca aagaacagca gggcgagttg gccggaaaag
     1981 ttggctcaac accaggctac ttacggctgg ttttcaatgg ttataaaaaa gccagttttg
     2041 tgctggctaa aaaacttgag caatgcacgt caggtgcaat tacgaaatct gacttaagac
     2101 cggatatcta tccgaaagat taacagaaca ccttcaattt ttaaccacag aacgatgagg
     2161 ctaaccgtgg gtaagtatca ctggaaagta gaaaaacagc ctgagtggta cgtgaaagct
     2221 gtcagaaaaa ctatcgcagc gttgccgggt ggttacgctg aagcagctga ctggctggat
     2281 gtaacagaga acgcattatt taaccgcctt cgtgccgatg gcgatcagat tttcccgctg
     2341 ggatgggcaa tggttttaca gcgcgcggct ggcactcact acattgcgga tgctgtcgca
     2401 cagtctgctg gtggggtgtt cgtatcgctt cctgaaattg aggaagtaga gaacgccgat
     2461 ataaaccagc gcctgctgga agtcatcgaa cagatcggga attactcaaa gcagattcgt
     2521 tcggcaatcg aagatggggt cgtggagcca cacgagcaga cagcaattaa tgatgagttg
     2581 tatctgtcaa tttcgaagct ccaggagcat gcagcactgg tctacaaaat cttttgcgct
     2641 ccagaaaaga gtgacgcccg cgagtgtgca gctccgggcg tcgtggcgtt ttgtgtctgt
     2701 ggagaaacta acgcatgaac agtttaacgg caaataaccg tttgtcgcaa cagctggtgg
     2761 tcagtgtcgc tgaacacctg ttgttacggc atgaatgcag attaccaaat cacctggctg
     2821 taagtaacca cagagaactt tacctgactg tggggggcga gttgtgcagg aacttaaccg
     2881 ctggtttcgt gacggaagag ggctttatgt ccatgttatt cgttgggagc cagaaacaca
     2941 gcgcgttatc tatcttcgca aagactaccc gcatgagtgc tttagtcctt tgtggaaatt
     3001 caggcgtgat tttgttgagt gtgaaggacc accagcacat tgattctgcc attccgggac
     3061 gttacactgt tcaggcacct tataaagcgg gtgccgggcg tggaaacccg gaattcacca
     3121 aagcgcacaa ccgcgctctt gcggtttttt tgtgtcatga gcagcattac gcccaaatta
     3181 tggtggggcg tgcagggcca acttcggttg ggccgggttc tttggtgacc ggtatttcca
     3241 cccctgtacg tctcaccacc aataaggtcg tggaaagcct tggtggtgag ttattaaaaa
     3301 tcaccaaaga ggctgccatc atggctacga tcccaaccct cactcaaccc gaaattgcca
     3361 tcgttgatgg tcaggctgtt acttcatccc tggctgttgc caacttcttc tccaaacgtc
     3421 atgacgatgt tctgaaaaag atccgcacgc ttgaatgctc cgcatcattc actgcccgca
     3481 atttttcggt gagtgattac accgattgca caggccgcaa actaccttgc tatcaaataa
     3541 cccgcgacgg ctttgcgttt cttgctatgg gtttcacggg taaacgtgct gcccagttca
     3601 aagaggcata catcaatgcc tttaaccaga tggagaaaca gctttcaaag ctcgctgtac
     3661 cgagtgacgt tgcacataac gccagcgttc tctattccta catttcatca attcatcagg
     3721 tctggttgca gcagctttat cccatgctgg aaaaagctga atcaccgctg gctgtaagtc
     3781 tgtatgaccg aattaacgat gcggcatttc ttgcccgtct tattcattcg tcgctgaact
     3841 cttcagaggt aagggggcgc aaatgatccg aaatattttc aaacggttta ccaatcaaac
     3901 tttccgttgt cctcgcccgg gtcagtggta caccacgcct gcagggcatg ttctacgtgt
     3961 tagcctggtt gaccgtgaat gtcagaaggt gatttgtgaa ccgctgggtc gtaattaccg
     4021 cgtcagtatg ccgctcatag cctttcgctc cggaaaaaac atgaagcatc tcggaggtgc
     4081 agcatgagta tggagctgat ggttaaagcg atgaaaattc gagtgggtaa tccattgcga
     4141 aaactggttc tgatcaagct ggctgataat gccagcgatc agggtgagtg ctggcccagc
     4201 taccagcata ttgctgacca gtgcgagatt agcaaacgtt ctgtgatgaa tcatattgcg
     4261 gccctttgtg agtccgggct ggtaaaaaaa gtcacccgga aaggtgaaaa aggtaactca
     4321 agtaatatct atctccttca tctggatggt gcaggagatt cactaggggg tagtgcaaat
     4381 aattcactat ctggtgcagc aaattcacca ggtagtgcag gagttgcacc agggggtagt
     4441 gcaggagatt cacccagaac cagtcactct tttgaaccag tcaaagaacc agtcaatgaa
     4501 ccaatagctg ttggtgcatc tgctgatgag tctgtgcgag ttcgttcaaa ccgaccggaa
     4561 tactctccgg agtttgagca ggcatggctg gcatacccca aacgtgctgg tggcaattca
     4621 aaatcagcag ccttcaaagc ctggaaagcc cgtttgaacg agggggtaaa ccccgaaacc
     4681 atgctggaag gtgtgaaacg ctacgcgggc tgggtatctg cgatgggcaa tagcggcaca
     4741 caatttgtga aacaggctgt cacgttcttt ggtccggatc gtcatttcga agaatcctgg
     4801 gaagttcctg cggtatctgc agccagacgc gatgacccgt acttcaaagc cagttacgac
     4861 aacgtggact acagccagat cccggcagga ttcagggggt gatcatgagt ctgttaaatg
     4921 acgttcagaa attcattgaa gcccatccgg ggtgtgcttc cggagacatt gcggatgctt
     4981 ttgcaggtta ctcacgacag cgcgttctgc agtcagcaag caagttacgt cagagtgggc
     5041 gtgtggctca ccgttgtgaa ggggatactc gcagacattt cccgcgccag actgaggttt
     5101 tgcaggaatc ggaaccgcaa ccagttcgtg aaaccagacc tgtgcgcaat ttctatgtcg
     5161 gcactaacga cccccgggtg attttgtgcc tgacccgcca ggctgaagaa ctggagtcca
     5221 ggggcttata ccgtcgtgct gcaacggtgt ggatggcggc attccgtgaa agccactccc
     5281 agccagaacg aaacaatttt ctggcgcgtc gtgagcggtg cttacggaaa agcagcaagc
     5341 gcgctgcatc gggtgaagag tggtatctgt cagggaatta cgtgggggct taatgagtaa
     5401 taaatattgc caggcgctgg tggaactgcg gaacaaacca gcccatgaac tgaaggaagt
     5461 gggcgatcag tggcgcacgc cggacaacat tttctgggga attaacaccc tgtttggccc
     5521 gtttgttctg gatctgttta ctgacggtga taacgccaaa tgtgccgcgt attacacggc
     5581 ggaagataac gcgctggcgc atgactggtc agaacgtctt gcggagctta aaggtgctgc
     5641 ctttggtaat cccccataca gccgcgccag tcagcatgag gggcaataca tcaccggcat
     5701 gcgttacatc atgaaacatg ccagttccat gcgtgataag ggcgggcgct atgttttcct
     5761 gatcaaagct gccaccagcg aagtgtggtg gccggaagat gcggaccata ttgcttttat
     5821 tcgcgggcgt attggttttg aactgcctgc ctggtttatc ccgaaggacg agaagcaggt
     5881 gccgacagga gcttttttcg ctggtgctat tgctgttttt gacaagacct ggaagggacc
     5941 ggcaatcagc tacatcgggc gcgatgaact tgaggcatgt ggtgaggcgt ttctggcgca
     6001 ggttcgccag caggcggaaa aactggtcag ggagatggcg gcatgacgac gttaactcaa
     6061 tgccagcagc aggtgctgga tatgctgatt tcttatcaga aagaacgtgg cttcccgcca
     6121 accaatcagg aggtggcaac catgctggga taccgttcgg tgaatgcagc ggtggagcat
     6181 cttcgcgcac tggagaaaaa aggcgtcatc acgataaagc gtggtgtggc ccgggggatc
     6241 acgcttcata ccgcggtgaa ggacgacgac agcgaggcgg tcgggattat ccgctcactg
     6301 cttgccggtg aggaaaacgc aaggctgcgt gcaactcact ggttacatga gagaggcctg
     6361 aaagtatgaa gctgatcctg cctttcccgc ccagcgtgaa cacgtactgg cgacacccca
     6421 acaaaggggc atttgctggt aagagcctga taagcgaggc ggggcgaaaa tttcagagcg
     6481 cggcgtgcgc agcaatagtt gagcagttac gtcgtctgcc gaaaccaacg tcggcacctg
     6541 cttcagtgga gatcgtgttg tttcctccgg ataaccggat ccgcgatctg gacaactata
     6601 acaaggcgct gtttgacgcc ctgacccacg cgggtgtgtg ggaagacgac agccaggtga
     6661 aaagaatgct ggtggagtgg ggaccggtta tcccgaaagg gaaggtcgag atcactatca
     6721 gtaagtatga gaaaccggcg ggtgcagccg cctgattaag aggagaaacg aagtatgaat
     6781 aatctgatgg tcattgatgg tattgaagtt cgtcgtgatg cttatgggcg ttacagcctg
     6841 aacgatctgc acagggcagc cgggggagaa caaaaaaaac gcccgaaata ctggctctcc
     6901 aataagcaaa cctgtgaatt gattgaacaa cttttcaccg agggtggaat tccgcctctg
     6961 gaaaagaatc aaccagttag cgttattaat ggcggaaata accaggggac gtatgtctgc
     7021 aaagaactgg tgtatgccta tgcaatgtgg atcagcccgt cattccatct gaaggtgatc
     7081 cgtactttcg atatggtaac cagcgcatcg gaaaaattat ccgggcaggc tgctgacaag
     7141 atgcaggctg gcgtgattct gctggacttt atgcgccggg agttaaacct gtctaactct
     7201 tcagtgcttg gtgcctgtca gaaactccag gaggctgttg gcttaccgaa tctggcaccg
     7261 cgctatgcca ttgatgctcc tgctgacgcg cctgatggct caagccgtcc cacgctgtca
     7321 ctgagtgcac tgctgaagca gtatggtatc cgcctgacag ctaatcaggc atatcaccag
     7381 atggtgaagc tggggatcgt cgagcagcgc gaacgataca gccgtaccgc gattaacaac
     7441 atcaaaaaat tctggtcgct gacagcgaaa ggctgcatgt tcggcaagaa catcaccagt
     7501 cccgcaaatc cgcgcgagac gcagccgcat ttcttcgaat cccgattccc tgagctgtta
     7561 aagctgctcg ataccgttca ttgaggtgac cgtgagagca ctactgaccc ctgaaattgc
     7621 cccgcgtatg gggatcgtat tgttcaggcc aggttcagag ctgatgcccc tgtttatgca
     7681 ggggcgtgtc ctgctggagc ctgagccgga acgttattca tctttcgcca gtggtgccgt
     7741 tccggcggca tcacaaccgc tggcggatga tcctgccgtt cgggccgtgt tccgcaatga
     7801 ggcagtgatc cgtcgtgctg gtggcgtgga atgtcttgaa agctggttac ttcgtgaaaa
     7861 aggctgccag tggcctcatt ccgactggca cagcgagaac atgaccacaa tgcgacacgc
     7921 gccgggcgca atccgtctgt gctggcactg cgataaccag ctgcgcgatc agttcacgga
     7981 acggctggaa tcaatggcaa cggataactg tgcccgctgg gtgttgtctg tcgtgcgtcg
     8041 ggatctcggt tttgatgaca gtcacgttgt gacaatgccg gaactgtgct ggtggctgat
     8101 tcgtaatgat ctggcggatg ccttaccgga aagtgcagcc cgtaaggtac tgagattacc
     8161 gaagcctgtt gtgccgtctg tcacccggga aagtgacctt gtgccttcgg ttcctgccac
     8221 cagcatcatc caggataaag cgaaaaaggt gctggcgctg aaagtggatc cggagtcgcc
     8281 ggagtctttt atgttacgcc caaaacgtcg ccgctgggtt aatgaaaagt acacgcgatg
     8341 ggttaagacg cagccgtgcg catgttgtgg aaaacctgct gatgatcccc accacctgat
     8401 aggtcacggt cagggtggaa tggctacaaa agcgcatgac ctctttgtgt tgcctttgtg
     8461 cagaaagcat cacgacgagc tgcatgcgga taccgtggca tttgaagaga agtatggctc
     8521 ccagctggag ctgatatttc gttttatcga tcgtgcactg gcaattggcg tgctggcctg
     8581 attttgtgga gaaagttgat gcgtgatatg tatgaagtat tggaccgctg gggtgcatgg
     8641 gctgcagcag ataacagtgg tgtggactgg cagcctgttg ctgcagggtt taaaggttta
     8701 ctacctcatg gaaagaaaac acgccaacaa tgcgatgatg atgaaggaat catgattgac
     8761 agctgtgttg cgcgattgcg gaaatataag cccgaagagt atgagttggt tattgctcat
     8821 tttgttatcg gcatctcact aagaactatt gcaaagaagc agaagtgttc tgatgggaca
     8881 ataagaaaag agttgcaaac agctcttggt tttctggatg gtgtgctttc aatgttgtaa
     8941 tattaggggg aattaccccc ctttttttct ctgttgcttt aataaaattt taatattttg
     9001 tctgattatg atgaggcaat gtaataaaag aaataccgtt agtattgcaa gccatacgca
     9061 aaataagcat gcatataaat tagttgaaag tccaatagtg aattgtgcaa ttgctgttgt
     9121 gatag
//
LOCUS       DAZGJW010000171         8996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZGJW010000171 DAZGJW010000000
VERSION     DAZGJW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.171"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            164..1222
                     /gene="citC"
                     /locus_tag="ACHKIK_004317"
     CDS             164..1222
                     /gene="citC"
                     /locus_tag="ACHKIK_004317"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO9085369.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1237..1533
                     /gene="citD"
                     /locus_tag="ACHKIK_004318"
     CDS             1237..1533
                     /gene="citD"
                     /locus_tag="ACHKIK_004318"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO9085370.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1530..2438
                     /gene="citE"
                     /locus_tag="ACHKIK_004319"
     CDS             1530..2438
                     /gene="citE"
                     /locus_tag="ACHKIK_004319"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO9085371.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2449..3981
                     /gene="citF"
                     /locus_tag="ACHKIK_004320"
     CDS             2449..3981
                     /gene="citF"
                     /locus_tag="ACHKIK_004320"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO9085372.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLATFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3985..4536
                     /gene="citX"
                     /locus_tag="ACHKIK_004321"
     CDS             3985..4536
                     /gene="citX"
                     /locus_tag="ACHKIK_004321"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO9085373.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4511..5389
                     /gene="citG"
                     /locus_tag="ACHKIK_004322"
     CDS             4511..5389
                     /gene="citG"
                     /locus_tag="ACHKIK_004322"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO9085374.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5440..6903
                     /gene="citT"
                     /locus_tag="ACHKIK_004323"
     CDS             5440..6903
                     /gene="citT"
                     /locus_tag="ACHKIK_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO9085375.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7017..7823
                     /gene="rna"
                     /locus_tag="ACHKIK_004324"
     CDS             7017..7823
                     /gene="rna"
                     /locus_tag="ACHKIK_004324"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO9085376.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8053..8463
                     /gene="rnk"
                     /locus_tag="ACHKIK_004325"
     CDS             8053..8463
                     /gene="rnk"
                     /locus_tag="ACHKIK_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO9085377.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8601..8690
                     /gene="yldA"
                     /locus_tag="ACHKIK_004326"
     CDS             8601..8690
                     /gene="yldA"
                     /locus_tag="ACHKIK_004326"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO9085378.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8694..>8996)
                     /locus_tag="ACHKIK_004327"
     CDS             complement(8694..>8996)
                     /locus_tag="ACHKIK_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085379.1"
                     /translation="FIHGFLFGDAFDKGLTFKMGQTHVHAWLGELLPLIEKGLLKPEE
                     IVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQSAEAAQKAVSGLVNAMPGGTI"
BASE COUNT         2069 a         2439 c         2315 g         2173 t
ORIGIN      
        1 ttcacgccga aatactcctt aggatgtata gcgaaaggag aaaaagatat acctcgatca
       61 cccccctttc tcccaagtga aaataaaagg ttatcagttt gcaacattga acaacattcg
      121 ttgcaaatcg ataacaacat gcaccttcag gatactattt attatgttcg gcaatgatat
      181 tttcacccgc gtaaaacgtt cagaaaataa aaaaatggcg gaaatcgccc aattcctgca
      241 tgaaaatgat ttgagcgttg acaccacagt cgaagtattt attaccgtaa cccgcgatga
      301 aaagcttatc gcgtgcggtg gaattgccgg aaatattatt aaatgcgttg ctatcagtga
      361 atccgttcgc ggtgaaggac tggcgctgac attagccact gaactgataa acctcgccta
      421 tgagcggcac agcacgcatc tgtttattta taccaaaacc gaatacgagg cgctgttccg
      481 ccagtgcggt ttttccacgc tgaccagcgt acccggcgtg atggtgctga tggaaaacag
      541 cgccacgcgg ctgaaacgct atgccgaatc gctgaaaaaa tttcgtcatc cagggaacaa
      601 gattggctgc attgtgatga acgccaatcc cttcacgaat ggtcaccgtt atctgattca
      661 acaagctgcg gcacagtgcg actggttgca tctgttttta gtcaaagaag attcttcacg
      721 cttcccctat gaagaccggt tggatctggt gttaaaaggc accgccgata ttccacgcct
      781 gactgtgcat cgcggctccg aatacatcat ctcccgcgct acgttccctt gctacttcat
      841 taaagaacag agcgtcatta accattgtta caccgaaatt gatctgaaga ttttccgtca
      901 gtacctcgct cccgcgctgg gtgtaactca ccgctttgtc ggtactgaac ccttttgtcg
      961 cgttaccgcc cagtacaacc aggatatgcg ctactggctg gaaacgccga ctatctccgc
     1021 accgcccatc gaactggttg aaattgagcg gctgcgttac caggagatgc cgatatccgc
     1081 ttcccgggta cgtcaactgc tggcgaaaaa cgatctcacg gctatcgcgc cgctggtccc
     1141 tgcagtcacg ctgcattatt tgcagaacct gcttgagcac tcccgccagg acgcggcagc
     1201 tcgtcaaaag acccccgcat gagaaacagg tgaaaaatga aaataaacca gcccgccgtt
     1261 gcaggcaccc ttgagtctgg ggatgtgatg atacgcatcg ccccactcga tacgcaggat
     1321 atcgacctgc aaatcaatag cagcgttgag aaacagtttg gcgatgcaat tcgcaccacc
     1381 attctggacg ttctcgcccg ctacaacgtg cgcggcgtac agctgaatgt cgatgacaaa
     1441 ggcgcactgg actgcatttt acgtgcacga ctggaagccc tgctggcacg cgccagcggt
     1501 atcccggctc tgccatggga ggattgccaa tgatttccgc ttcgctgcaa caacgtaaaa
     1561 ctcgcacccg ccgcagcatg ttgtttgtgc ctggtgccaa tgccgcgatg gtcagcaact
     1621 ccttcatcta cccggctgat gccctgatgt ttgacctcga agactccgtg gcattacgtg
     1681 aaaaagacac cgcccgccgc atggtttatc acgcgctgca acatccgctg tatcgcgata
     1741 ttgaaaccat tgtgcgtgtc aacgcgctgg attccgaatg gggtgttaac gacctggaag
     1801 ccgtcgttcg cggtggtgcg gacgttgtgc gtctgccgaa aaccgatacc gctcaggatg
     1861 ttctggatat tgaaaaagag atcctgcgta tcgaaaaagc ctgtggtcgt gaacccggca
     1921 gcaccggcct gctggcggcg attgaatctc cgctgggcat tacccgcgca gtggaaatcg
     1981 ctcacgcttc cgagcgtttg atcggtatcg ccctcggtgc agaagactat gtgcgcaacc
     2041 tgcgtacaga acgctccccg gaaggaactg aactgctgtt cgcacgctgt tccattttgc
     2101 aggccgcgcg ctctgcgggt attcaggcgt tcgataccgt ctattccgac gctaacaacg
     2161 aagccggatt cctgcaagaa gccgcccaca tcaaacagct gggctttgac ggcaaatcgc
     2221 tgatcaaccc gcgtcagatt gatctgttgc acaacctcta cgcaccaacc cagaaagaag
     2281 tggatcacgc ccgccgcgtc gtagaagccg ctgaagccgc cgctcgcgaa ggcctcggcg
     2341 tggtttccct gaacggcaag atggtggacg gtccggttat cgatcgcgcc cgtctggtgc
     2401 tctctcgtgc agaactttcc ggcatccgcg aagaataagg caatcaaaat gacgcagaaa
     2461 attgaacaat ctcaacgaca agaacgggta gcggcctgga atcgtcgcgc tgaatgcgat
     2521 cttgccactt tccagaactc gccaaagcaa acctaccagg ctgaaaaagc gcgcgatcgc
     2581 aaactgtgcg ccaacctgga agaagcgatt cgtcgctctg gtttacagga cggcatgacg
     2641 gtttccttcc atcacgcttt ccgtggcggt gacctgaccg tcaatatggt gatggacgtc
     2701 atcgcgaaga tgggctttaa aaacctgacc ctggcgtcca gctccctgag tgattgccat
     2761 gcgccgttgg tagaacacat tcgccagggc gtggttaccc gcatttatac ctccggcctg
     2821 cgcggtccac tggcggaaga gatctcccgt ggtctgctgg cagaaccggt gcaaatccac
     2881 tctcacggcg gtcgcgtgca tctggtacag agcggcgaac tgaatatcga cgtggctttc
     2941 ctcggcgtcc cgtcctgcga tgaattcggt aatgccaacg gctacaccgg taaagcctgc
     3001 tgcggctccc tcggctatgc aatggttgat gccgacaacg caaagcaggt cgtgatgctt
     3061 accgaagaac tgctgcctta tccgcataat ccggcaagca ttgagcaaga tcaggttgat
     3121 ttgatcgtca aagttgaccg cgttggcgat gctgcaaaaa tcggcgctgg cgcgacccgt
     3181 atgaccacta acccgcgcga actgcttatt gcccgtagcg ctgcggatgt gattgtcaac
     3241 tcaggctact tcaaagaagg tttctccatg caaaccggca ccggcggcgc atcgctggcg
     3301 gtaacccgtt tcctggaaga caaaatgcgt agccgcgata ttcgcgccga cttcgccctt
     3361 ggcggtatta ccgcgacgat ggttgacctg cacgaaaaag gtctgatccg caaactgctg
     3421 gatgtgcaga gctttgacag ccatgctgcg caatcgctgg cccgtaaccc caatcacatc
     3481 gaaatcagcg ccaaccagta cgctaactgg ggttcgaaag gcgcatcggt tgatcgtctc
     3541 gacgtggtgg tactgagcgc gctggaaatt gacacccagt tcaacgttaa cgtgctgacc
     3601 ggctctgacg gcgtactgcg tggtgcttcc ggtggtcact gcgataccgc gattgcctct
     3661 gcgctttcca tcatcgtcgc gccgctggta cgcggtcgta ttccgactct ggtggataac
     3721 gtgctgacct gcatcacccc gggctccagt gtcgatattc tggtcacaga ccacggtatc
     3781 gcagttaacc cggcacgtcc ggaactggca gaacgtctgc aggaagcggg cattaaagtg
     3841 gtttccattg agtggctgcg cgaacgtgcg cgtctgctga ccggtgaacc acagccgatt
     3901 gaattcaccg accgcgtcgt tgccgttgtg cgttaccgcg atggctcggt gatcgatgtt
     3961 gtgcatcagg tgaaggaata agccatgcac ctgcttcctg aactcgccag ccaccatgcg
     4021 gtgtcaattc ccgagctgct cgtcagccgg gatgaaaggc aagcacggca acacgtctgg
     4081 ctcaagcgcc atcctgttcc actggtctcc tttaccgtgg ttgcgcctgg gccgattaaa
     4141 gacagcgagg tcacacgccg aatttttaat catggcgtga cagccttgcg tgccttagcc
     4201 gcaaaacagg gctggcaaat tcaggagcag gctgcactgg tttccgccag cgggccggag
     4261 ggcatgttga gcattgccgc cccggctcgc gacctcaagc tcgccaccat tgagcttgaa
     4321 catagtcatc ctctcgggcg gttatgggat atcgatgtcc tgacgcccga aggcgaaatt
     4381 ctctcccgcc gcgactattc actgccgcct cgccgctgcc tgttgtgcga gcaaagcgca
     4441 gccgtctgcg cgcgtggaaa aacccatcaa ctgaccgatt tactcaaccg catggaggca
     4501 ctgctgaacg atgtcgatgc ctgcaacgtc aactaaaacc acaaagcttg cgacgtcatt
     4561 aatcgatgag tacgccctgc tgggctggcg cgccatgctg actgaagtca atctgtcacc
     4621 gaaaccaggc ctcgtggatc gcattaactg cggtgcgcac aaagatatgg cgctggaaga
     4681 tttccaccgc agcgcgctgg cgattcaggg ctggttaccc cgtttcattg aatttggtgc
     4741 ctgtagtgca gaaatggcac cggaagcggt actccacgga ttacgcccaa ttggtatggc
     4801 ttgcgaaggt gatatgttcc gcgccactgc gggcgtaaac acgcataaag gcagcatttt
     4861 ttctttaggg ctgctatgtg cggcaattgg ccgtttgctt cagctcaacc agccggtaac
     4921 gccaacaacc gtttgttcta cggcggcaag tttctgccgt ggtctgaccg atcgcgaact
     4981 gcgtaccaat aattcacaac tgacggcagg tcaacggttg taccagcagc ttggccttac
     5041 cggcgcacgc ggtgaagccg aagcgggtta tccactggtg atcaaccacg ccttgccgca
     5101 ttacctcact ctgctggatc aggggttaga tcctgaactg gcattgctcg ataccttgct
     5161 cctactgatg gcgatcaacg gcgataccaa cgttgcttcg cgcggtggcg aggggggcct
     5221 gcgctggcta cagcgcgagg cgcaaacatt attgcaaaaa gggggcattc gaacccccgc
     5281 cgatctcgat tatctccggc agttcgacag ggagtgtatc gaacgaaatc tcagtccagg
     5341 cggcagtgcc gacctgctga tccttacctg gtttttagca cagatttaat tatttaagca
     5401 cttgataaat ttgaaaatat taattttcgg agaacccgta tgtctttagc aaaagataat
     5461 atatggaaac tattggcccc actggtggtg atgggtgtca tgtttcttat ccctgtcccc
     5521 gacggtatgc caccacaggc atggcattac ttcgctgtgt ttgtggcaat gattgtcggc
     5581 atgatcctcg agccaattcc ggcaacagcg atcagtttta ttgcggttac tatttgcgtt
     5641 attggcagta attacctgct ctttgatgcc aaagaattag ctgacccagc gtttaatgcg
     5701 caaaaacagg cgctgaaatg gggtctggct ggtttttcca gcaccacggt atggctggta
     5761 tttggcgcat ttatttttgc attagggtat gaagtttccg ggttaggtcg tcgcattgcc
     5821 cttttcctgg tgaaattcat gggcaaacgc acgctgacgt tgggttatgc gattgtcatt
     5881 atcgacattc tgctggcacc gtttacaccg tccaacaccg cgcgtaccgg gggtacggtt
     5941 tttccggtca ttaaaaacct gccgccgttg tttaaatcat tccccaacga tccgtccgcg
     6001 cgtcgtattg gcggctattt gatgtggatg atggtcatta gtaccagtct gagttcgtcc
     6061 atgtttgtca ccggtgcggc accaaacgtg ctgggtctgg agttcgtcag caaaattgcc
     6121 ggtatccaga ttagctggtt gcagtggttc ctctgcttcc tgccggttgg ggttatcttg
     6181 cttatcattg cgccgtggct ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa
     6241 gaagtggcaa cctgggcggg tgatgaactg aaaaccatgg gtgcgctgac acgcagagag
     6301 tggacactga ttggccttgt attgctcagc ttaggtttgt gggtttttgg cagcgaagtc
     6361 attaatgcta ctgcagttgg tctgctggca gtttcgctaa tgctggccct gcacgttgtg
     6421 ccttggaaag atattacccg ctataacagc gcatggaaca cgctggtcaa cctagcaact
     6481 ctggttgtga tggctaacgg cctgactcgt tctggcttta ttgactggtt cgccggtacc
     6541 atgagtacgc acctggaagg attctcacca aacgcaacgg tgattgtact ggttctggtg
     6601 ttctactttg cacactacct gtttgccagc ctgtctgcgc acaccgcaac catgctgccg
     6661 gttattctgg ccgtcggtaa aggtattccg ggcgtaccaa tggaacaact gtgtatcctg
     6721 ctggtgctgt ctatcggtat catgggctgt ctgacgccgt atgcaaccgg tcctggggtg
     6781 attatttacg gctgtggcta tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc
     6841 ggggtgattt acatctctat gttgctgttg gttggctggc cgattctcgc catgtggaac
     6901 taatacgttc gccaaacgct gaataaatca tcacgcccgc caggtaagcc cacctggcgg
     6961 gcttttttat gatttaatag atagtattca gtgtatgtat gagttccaca cccattatga
     7021 aagcattctg gcgtaacgcc gcgttgctcg cggtttctct gcttcccttc tcttctgcca
     7081 acgccttagc gttgcaggca aaacagtatg gcgattttga tcgctatgtc ctggccctct
     7141 cctggcaaac cggattttgc cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat
     7201 gtcgcctgca aaccgaaacg accaacaaag ctgattttct gaccgtacat ggtctgtggc
     7261 caggattgcc taaatcggtt gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg
     7321 gttgcgctac tcgcccaatc ccgaatctac cagaagcgca cgccagccgg atgtgttcat
     7381 cgccggaaac cggattatca ctggaaacgg ccgctaaact aagtgaagtc atgccaggag
     7441 ctggcggacg ttcctgcctg gaacgctacg aatatgccaa acacggtgcc tgctttggtt
     7501 ttgatccgga cgcatacttc ggtacgatgg tacgcctgaa tcaagaaatt aaagagagcg
     7561 aagccggaaa attccttgcg gataattacg gtaaaacagt gagccgccgt gactttgacg
     7621 ccgcctttgc caaaagctgg ggaaaagaga acgtgaaagc agttaagcta acgtgccagg
     7681 gtaaccctgc gtatttgact gaaattcaga tctcgatcaa agctgacgca atcaacgctc
     7741 cgctttctgc aaactcattc ttgccacaac cacacccagg caactgtggc aaaacctttg
     7801 tgattgataa agtgggttat taatcatctg atttacaaaa cacgctaatt cgtcttccat
     7861 aaaccgtgac gattaagaat tatacataat gcgctgcatt gaggaattcc ctgaatgtga
     7921 cgcaaatcac ttcaaagagt gagcaacagt cagtatcatc atgtgagtta aaccctcgcc
     7981 gcctgacggt gagggttttc ttttgggtat gtttatctgc gacactcgca gtaccgacga
     8041 catggagtaa aaatgtccag accaactatc atcattaacg acctggatgc cgaacgcatc
     8101 gatattctgc tggagcaacc cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca
     8161 gagttggatc gcgcccaaat gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg
     8221 aacagccggg ttaaattccg caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg
     8281 tatccggcaa aaatgaccga tagcaatact cagctttccg ttatggctcc ggtaggtgcc
     8341 gcactgctgg ggctgcgcgt tggcgattcc attcactggg aacttccggg cggcgttgca
     8401 acccaccttg aagtgctgga actcgaatac cagccagaag ctgctggcga ctacctgctt
     8461 taatcccatc tgcacaagcg ataactcgct ttgcccagag gatgcactcg catcctcttt
     8521 acgcaaaatc agcaattttt cctggttctc actgttcact tttgctgcaa aattattccg
     8581 tttcgtttgt gggaataacc atggcagaag cattttacat cctgatagga tttctgatta
     8641 tggcggcgat tatcgtcatg gctgtccttt acctcgaaaa ccactcctga cgatcatatt
     8701 gttccccccg gcatcgcatt caccagacct gaaaccgcct tctgcgccgc ctctgcgctt
     8761 tgtgcaccgg gtaccagaat caccttacgg cactcctctt cacgtttttc gaaaatctca
     8821 tatccccggg cggcctcttc aaacggcata tagtgggtaa caatttcttc tggtttcagt
     8881 aatcctttct caattaacgg taataattct cccagccatg cgtgaacgtg ggtctgtccc
     8941 attttaaacg tcaacccttt atcaaaggcg tcgccaaaca ggaaaccgtg aataaa
//
LOCUS       DAZGJW010000172         8926 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZGJW010000172 DAZGJW010000000
VERSION     DAZGJW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.172"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1047
                     /locus_tag="ACHKIK_004328"
     CDS             <1..1047
                     /locus_tag="ACHKIK_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO9085380.1"
                     /translation="KPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNIL
                     DNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKIL
                     VCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEV
                     SSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVM
                     LVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSA
                     QAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALRE
                     AKSEGGNKVIIHHI"
     gene            1228..2718
                     /gene="dgcJ"
                     /locus_tag="ACHKIK_004329"
     CDS             1228..2718
                     /gene="dgcJ"
                     /locus_tag="ACHKIK_004329"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO9085381.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2761..3264
                     /gene="yeaK"
                     /locus_tag="ACHKIK_004330"
     CDS             2761..3264
                     /gene="yeaK"
                     /locus_tag="ACHKIK_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO9085382.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3265..3369)
                     /gene="yoaI"
                     /locus_tag="ACHKIK_004331"
     CDS             complement(3265..3369)
                     /gene="yoaI"
                     /locus_tag="ACHKIK_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO9085383.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3539..3985
                     /gene="yeaL"
                     /locus_tag="ACHKIK_004332"
     CDS             3539..3985
                     /gene="yeaL"
                     /locus_tag="ACHKIK_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO9085384.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(3942..4763)
                     /gene="nimR"
                     /locus_tag="ACHKIK_004333"
     CDS             complement(3942..4763)
                     /gene="nimR"
                     /locus_tag="ACHKIK_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO9085385.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4860..6041
                     /gene="nimT"
                     /locus_tag="ACHKIK_004334"
     CDS             4860..6041
                     /gene="nimT"
                     /locus_tag="ACHKIK_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO9085386.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRTLHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6096..6443
                     /gene="yeaO"
                     /locus_tag="ACHKIK_004335"
     CDS             6096..6443
                     /gene="yeaO"
                     /locus_tag="ACHKIK_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO9085387.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6465..6719)
                     /gene="yoaF"
                     /locus_tag="ACHKIK_004336"
     CDS             complement(6465..6719)
                     /gene="yoaF"
                     /locus_tag="ACHKIK_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO9085388.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            6902..7927
                     /gene="dgcP"
                     /locus_tag="ACHKIK_004337"
     CDS             6902..7927
                     /gene="dgcP"
                     /locus_tag="ACHKIK_004337"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO9085389.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            7960..8058
                     /gene="yoaK"
                     /locus_tag="ACHKIK_004338"
     CDS             7960..8058
                     /gene="yoaK"
                     /locus_tag="ACHKIK_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO9085390.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8061..8135
                     /gene="yoaJ"
                     /locus_tag="ACHKIK_004339"
     CDS             8061..8135
                     /gene="yoaJ"
                     /locus_tag="ACHKIK_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO9085391.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8194..8442)
                     /gene="yeaQ"
                     /locus_tag="ACHKIK_004340"
     CDS             complement(8194..8442)
                     /gene="yeaQ"
                     /locus_tag="ACHKIK_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO9085392.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8670..>8926)
                     /locus_tag="ACHKIK_004341"
     CDS             complement(8670..>8926)
                     /locus_tag="ACHKIK_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO9085393.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSQFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         2205 a         2163 c         2107 g         2451 t
ORIGIN      
        1 aaaccaatta acggcagtac actgatccag acaaagtcga atgatgtttc tattttctat
       61 attttccgcc aactcagttt tatttgttta acctcgctgg cactcttttg ttatggaaaa
      121 gacaacatcc ttgacaacaa taagaaaaaa acgggaatcc tgttgctggc gctgattcct
      181 tttttagttt ttccccttct ggcacacaat ctgagcagtt ataacgctga ctattctttg
      241 tatgtcgtcg actactgtcc ggacaaccat actgcgacct ggggaatcaa ctatacaaaa
      301 atattggttt gtctgtgggc atttttactg ttctttatta tcatgcgtac acgattagcc
      361 agcgaactat ggccgttaat agcattatta tgtctggcat cgctatgctg caacttactt
      421 ctactgactc tggatgagta taattacacc atctggtata tcagtcgcgg gattgaagtt
      481 tccagtaaac tgtttgttgt gtcttttctg atttataaca tttttcagga actgcaactc
      541 tccagcaaac tggcagttca tgatgtgctg accaatattt ataatcggcg ctactttttc
      601 aacagcgtag agtcattatt gtcgcgacct gttgttaagg acttctgtgt catgctggtt
      661 gatattaatc agttcaaacg catcaatgcc caatggggac atcgtgtggg tgataaagtg
      721 ctggtttcaa ttgtcgatat tatccagcaa agcatccgcc ctgatgatat tttagcgcga
      781 ctggagggtg aggtgtttgg cttgctattt accgaactca atagtgccca ggcaaaaatc
      841 attgcggaac gtatgcgtaa aaatgtcgaa ctcctgaccg gctttagtaa cagatatgat
      901 gttcctgaac aaatgaccat cagtattggc acggtttttt caacgggtga cacgcgtaat
      961 atctcgcttg tcatgacgga agcagataaa gccttacgcg aagcgaaaag cgaggggggc
     1021 aacaaagtga ttattcatca tatttaagtg caaaaatatt cagagccatg ctttttgcgt
     1081 ggcttttgca tacaatttat tactattacc cctaaatttc ccctccccag cggcgcagag
     1141 atgagtataa ttagcgcccc tgtgccaggc cgcaatcgaa ctttatctgg ttttctcgtt
     1201 tcactaaccg aaggagtgcc atttatcatg aaattgcacc atagaatgct ccggcatttt
     1261 atcgccgcaa gtgtcattgt gctgacatct tccttcctta tttttgaact tgtcgccagc
     1321 gacagagcaa tgagtgccta tctgcgctat atcgtgcaga aagcagactc ctcctttctt
     1381 tatgataagt atcagaatca gagtattgcc gcgcatgtga tgcgcgctct cgctgctgag
     1441 cagtcggaag tgtcgccaga acagcggcgc gccatctgcg aggcttttga gtctgccaat
     1501 aatacccatg gcttaaacct gactgcccat aaatacccgg gcttacgcgg cacactacaa
     1561 accgcatcca ctgactgcga cacaattgtg gaagctgcag cactattacc cgcttttgat
     1621 caggcagtgg aaggcaaccg ccaccaggat gattacggtt caggtcttgg gatggccgaa
     1681 gagaaatttc actattatct cgatctcaat gaccgctatg tctattttta tgagccggtt
     1741 aatgttgaat actttgcgat gaataactgg tccttcctgc agtcaggaag tattggcatc
     1801 gatcgcaaag atattgaaaa ggtatttacc gggcgtaccg tattgtcgag catttaccag
     1861 gatcagcgta ctaaacagaa cgtgatgagt ttgctgacgc cggtatatgt cgcagggcag
     1921 ctaaaaggga ttgtgctgct ggatattaac aaaaacaatt tgcggaatat cttttacact
     1981 catgaccgcc ctctcctctg gcgttttctc aatgtcacgc taaccgatac cgattcgggg
     2041 cgcgacatta tcatcaacca gagcgaagat aatctgttcc agtacgtcag ttacgtccat
     2101 gacttaccgg gcggcattcg tgtctcgtta tccattgata ttctttactt tatcacgtct
     2161 tcgtggaaaa gcgttctgtt ctggattttg acggcgttaa ttttgctgaa tatggtccgg
     2221 atgcacttcc gtttatacca aaatgtgtcg cgagaaaata ttagtgatgc gatgactgga
     2281 ctgtataatc gcaaaatttt aacccctgaa ctggagcagc ggttgcagaa actggtgcaa
     2341 tccggttctt cggtgatgtt tattgcaatt gacatggaca agttaaagca aataaatgac
     2401 accctcggtc atcaggaggg ggatttagcg attacgttat tagctcaggc gattaaacaa
     2461 tcgattcgta aaagtgatta tgccatccga ctcggtggcg atgaattctg catcattctt
     2521 gtcgattcga cgccgcaaat tgcagcacaa ctgcctgaac gtatcgaaaa acgtctgcaa
     2581 catatcgcgc cgcagaaaga aatcggcttc tcttccggta tttacgcgat gaaagaaaac
     2641 gatacgttac atgatgcgta taaagcttcc gatgagcgtt tatatgtcaa taagcagaac
     2701 aaaaacagcc gttcatgata accttctgtg gttgtttgct tgtaatctca ggagcgtgaa
     2761 atgactgaaa tggctaaagg aagcgtgacg catcagcggt taatcgcttt attatcacaa
     2821 gaaggtgcta acttccgcgt cgttacccat gaagcggtag gtaaatgtga agcggtgtca
     2881 gaaattcgtg gcaccgctct aggccagggt gctaaggcac tggtatgtaa agtcaaaggc
     2941 aatggcgtaa atcaacatgt tctggcgatc ctcgctgccg atcaacaggc cgatctgagc
     3001 caactcgcca gccatatagg cggattacgc gcttcgttgg ccagcccggc ggaagtcgat
     3061 gaactcacag gctgtgtctt cggcgcgatc ccccctttca gcttccatcc aaaactcaaa
     3121 ctggttgccg accctctact cttcgaacga tttgatgaaa tcgccttcaa tgcagggatg
     3181 ctggataaat ccgttatttt gaaaaccacg gattatttgc gcattgcgca accagaactg
     3241 ctcaatttcc gccgcactgc gtaactagcc tgtccgttca ataagcagaa cggacaaaac
     3301 cagcacaaca gcaatggcaa aaaacgatga cgtgataatc agtgtctcga cgaacatttg
     3361 atcgttcata gcatgccccc gggagatagc tgttttcacg ttattgttag cgtgcacaaa
     3421 tggcagtttg atgacagttc gcctgatttt ttatgcaaaa aacgtaaaga ttttctactt
     3481 ccttcctgca gcaagcgtaa agtaagcagg cttattattt tttggcaagg aaaccacgat
     3541 gtttgatgtc actttgctga tcctgctcgg gttagctgcg ctgggcttta tcagtcataa
     3601 caccactgtc gccgtttcaa ttctggtgtt gatcattgtc cgcgtcacac cgttaagcac
     3661 ctttttcccg tggattgaaa aacaggggct aagtatcggg ataatcattc tgacgattgg
     3721 cgttatggcc cccatcgcca gcgggacgct accgccctca accttgattc actcgtttct
     3781 gaactggaaa tcgctggtgg caattgcagt aggcgttatt gtctcctggc tgggtgggcg
     3841 cggcgtgacc ttaatgggca gccagccgca actggtcgcc gggctgctgg taggcactgt
     3901 tttaggcgtg gcgctgtttc gcggcgtacc ggtcggcccg cttattgccg cgggtctggt
     3961 ttcgctgatt gtggggaaac agtagttaat cccgcaatat atcggcctgg cgtttgcccc
     4021 aggcctttct taaacatagt aataaaggcc gttgttgaat cataccccag cgtatgagca
     4081 actttctgta cagtatcgcc tttcactaat ccctgaagtg ccataatcag ctgtaactgc
     4141 tgacgccact ggcgaaaact caaccccgtc tcttttacta tcagtcgcgc aagattgcgt
     4201 tcactcatcg caaaaaagcc agcccattgc cctaatgacc cccactcgac gggccctttc
     4261 gccatcatct ccaccatcgt gcggatttta ggatgagaag aaacgggcag gtgcagctgt
     4321 tgttgtggct gctgagggag ttcatcaaac aggacctgaa ttaatctgcg ggtcatcggc
     4381 tcagcacgct gagtcgtcgt cctgtttgcc agggttaaaa tcaattcccg gcataagggc
     4441 gatattttca atgtgcaaca tgttgtcggc attgtcactg cgctgggttc gataaacaga
     4501 aaacagagtt cagcgttagc tgtcacttga ttgctatgtt ccaccccgcc cggtatccaa
     4561 accgcatatt gcggcggcac catccacaaa gcattttcca ccgtacaggt aattgcacca
     4621 tgtaatgcta gtatcagctg tcctttgcgg tgctgatgta caggactggt taactcatcc
     4681 gttccggcgt gaatacaaaa tgcgaccgcg gcttcatggt gtcggtcggg ttcatagcca
     4741 ttgagattca acctgtgcat cattttgtcc gaatttagcg ataatttgtc attttagctt
     4801 gattcaacat aacaataaaa acggtaaggt acagcctcgt ttgtaacaat gagaagcata
     4861 tgacctgttc aacttcatta agcggcaaaa acaggattgt ccttatcgct ggcattctga
     4921 tgattgccac tacattacgc gtcaccttca ccggcgcagc accgttactc gatacgattc
     4981 gttccgctta ctcgctgacg acagcgcaaa ccggcttatt aaccaccctg ccattattgg
     5041 cctttgcgct aatctcacct ttggctgccc cggtggcgcg acgttttggt atggaacgta
     5101 gcctgtttgc cgcgttactt ttgatctgtg caggtatcgc aattcgttct ctcccttcgc
     5161 cttacttatt atttggcggt acagcggtca ttggcggtgg gattgcatta ggcaatgtct
     5221 tactgccagg attaattaaa cgcgatttcc ctcattccgt cgccagactt accggcgcat
     5281 attccctgac aatgggagct gcagcggcac tgggatcggc tatggtcgtg ccgctggctt
     5341 tgaacggttt tggctggcaa ggcgcgttgt tcatgctgat gtgttttcct ctgctggctc
     5401 tttttttatg gctgccacag tggcgaagcc aacaacatgc aaatttgagt acctcgcgca
     5461 ccttacatac tcggggtatc tggcgttcgc cgcttgcctg gcaggtcaca ttgtttcttg
     5521 ggatcaactc actggtctat tacgtgatta ttggctggct tccggcgatc ctcatcagtc
     5581 gcggctatag cgaagcacag gcgggttcac tgcatggttt gctgcaacta gccacagcag
     5641 ctcccggttt gctgatccct cttttcttac atcatgtgaa agatcagcgt ggtattgcag
     5701 cgttcgttgc cttgatgtgc gcagtgggcg ctgttgggct ctgctttatg ccagcgcacg
     5761 cgatcacctg gactctgctt ttcggttttg gttccggcgc aacaatgata ctggggttga
     5821 cgttcattgg tcttcgggca agttctgcgc atcaggcggc ggcactctcg gggatggcac
     5881 aatccgtcgg gtatttgctg gcagcctgtg ggccgccgct gatgggtaaa atacacgatg
     5941 ctaacggtga ctggtccgta ccactgatgg gtgttgccat actttcacta ctgatggcga
     6001 ttttcggact ttgcgccggg agagacaaag aaattcgcta atatccggtg ctatagtgac
     6061 gtgacaaatc atgcgtgaaa gggagaacaa acacgatgaa tattcagtgc aaacgcgttt
     6121 atgatccggc tgaacagagc gatggttatc gcgtactggt cgaccgcctc tggccgcgcg
     6181 gtatcaaaaa aaccgattta gcccttgatg agtgggataa agaaatcacg ccgtcaacgg
     6241 aactgcgcaa agcctttcac ggcgaagtcg tcgattttgc aacctttcgc gagcaatatc
     6301 ttgcagaact ggcgcaacac gagcaagaag gaaagcggct ggcggacatc gccaaaaaac
     6361 agccgctgac cctgctctac gcagcaaaaa acaccacgca gaaccatgcg ctggtgctgg
     6421 ccgactggct acgtagcttg tgattttagt acagcatccg gcggttattt ttcaccagcc
     6481 ggatggtccc gtcgccacaa tgcccattca tcaatcgttt caccgcccgg taatttgcaa
     6541 ttgttgctga ccccttgcgc tgtctgcact ggaatgagcg tcccgccctt ctgctggcaa
     6601 tagaccgacg ccggatttgc cataccaatc tgcggcggtt taggtgcttc tggctgagaa
     6661 gaggttgaac aaccagccag gaccagcaag caaggcaaag caaaactgat aattttcatt
     6721 tattgatctc acacatttat ctacgcttag agtatcgcgg tatcgttttg ttttgcagca
     6781 ctatttttat tacattcact caaaacatat tacgtcttgt ttcatctttg ttgatgatgt
     6841 tttatcatgc ctgcaaagat taaataatca gcatttaccc gccgtatcct ggagttgttt
     6901 cgtgtcagat cagattatcg cccgcgtctc gcaatccctt gccaaagaac agtcactaga
     6961 aagtctggtc cgacagcttc tggagatgct ggaaatggtc actgatatgg aatcaaccta
     7021 cctgaccaaa gtggatgtcg aagcgcgcct gcagcatatt atgtttgccc gtaacagcca
     7081 gaaaatgcac atcccggaga attttaccgt ctcgtgggat tactcgttat gcaaacgcgc
     7141 tattgatgaa aactgctttt tcagcgatga agtccctgac cgttggggcg actgtattgc
     7201 ggcacgcaat cttggaatca ccacatttct gagtacacca attcacttac cggatggatc
     7261 attctatggc acgctttgcg ccgccagcag tgagaagcgc cagtggagtg aacgcgcgga
     7321 acaggtttta cagttattcg ccggactgat tgcacaatat attcaaaaag aggcgttggt
     7381 tgaacagctg cgcgaagcca atgccgcgct gattgcgcaa tcgtataccg actcgttaac
     7441 cgggctaccg aatcggcggg cgatttttga aaatctgacg acgctgtttt ctctcgcccg
     7501 gcatcttaac cataagataa tgatcgcgtt tatcgatctg gataacttca aattaatcaa
     7561 tgatcgtttt ggtcataata gtggcgatct gtttctcatt caggttggcg agcgccttaa
     7621 tacgctccag caaaatggcg aagttattgg tcgtctcggc ggtgatgagt ttttggttgt
     7681 ttcactaaat aacgagaatg cggatatttc gtcgctgcga gaacgtattc aacagcaaat
     7741 acgtggagaa tatcacttag gtgatgttga tttgtattat cctggtgcca gtcttggcat
     7801 tgtagaagtc gatcctgaaa cgaccgatgc agacagtgcc ctgcatgctg ccgatatcgc
     7861 gatgtatcag gagaaaaaac acaaacagaa aacacctttt gtcgcgcatt cagcgccaca
     7921 ttcctgaggc gtattcacat ccttttgatt ggtgataaca tgcgaatcgg tattattttt
     7981 ccggttgtaa tctttattac agcggtcgta tttttagcat ggttttttat tggcggctat
     8041 gctgccccgg gagcataaag atgaaaaaaa caacgattat tatgatgggt gtggcgatta
     8101 ttgtcgtact cggcactgag ctgggatggt ggtaacgtca cctctaaaaa atagcaaagg
     8161 ctgcctgtgt gcagcctttg tgcaatttaa gcgttaactt ttaatcttcc tgtagataaa
     8221 tagcacgaca atcgcaccaa taacggcaac cacgaagctg ccaaaattga agccatcgac
     8281 tttaccaaag ccaaacagcg tgctgatcca tccgccaact acggcaccga ctatccccag
     8341 caggatagtc ataaagaatc caccaccatc tttacctggc atgatccact tcgccagaat
     8401 accggcaata agcccaaaaa taatccatga cagaatgccc attgtttcct cacttatctg
     8461 ttttgcatta gcgggttagt cgctgataaa aagcatagca caacatcggg agggcaagat
     8521 ttgtgacgag catcacggag gatttttttg cggatggcac agaaattgcg ccatcaacga
     8581 tcagtgataa ttaccaacca caaacatcat gttcgttttc cgtgtcttga catcctccac
     8641 gccctgaatg acgaggatcc ctgctacgtt caggctgtcg cctgaatcat ttcggtgggt
     8701 tcctgcttca acgggcggcc tgactgcacc atctctccac aggcaagaac ggcgtgcccc
     8761 gccgctaaaa tgttacgagc gccgtttaca tcggcgttcg ctgtatatcc acatacctgg
     8821 catctgaatt gactttgtga caggcgattt tctttcgctg tatgaccaca gcacgcgcaa
     8881 cgctggcttg tgtacgctgg cggaacagca agcacctgac cgccac
//
LOCUS       DAZGJW010000173         8858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZGJW010000173 DAZGJW010000000
VERSION     DAZGJW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.173"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(129..3491)
                     /gene="mscK"
                     /locus_tag="ACHKIK_004342"
     CDS             complement(129..3491)
                     /gene="mscK"
                     /locus_tag="ACHKIK_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO9085394.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(3619..4266)
                     /gene="acrR"
                     /locus_tag="ACHKIK_004343"
     CDS             complement(3619..4266)
                     /gene="acrR"
                     /locus_tag="ACHKIK_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO9085395.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQVKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            4408..5601
                     /gene="acrA"
                     /locus_tag="ACHKIK_004344"
     CDS             4408..5601
                     /gene="acrA"
                     /locus_tag="ACHKIK_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO9085396.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            5624..8773
                     /gene="acrB"
                     /locus_tag="ACHKIK_004345"
     CDS             5624..8773
                     /gene="acrB"
                     /locus_tag="ACHKIK_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO9085397.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDREKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
BASE COUNT         2127 a         2268 c         2318 g         2145 t
ORIGIN      
        1 aataaacttc aatatattga atttgcatga ctttgtaggc cggataaggc ggtcacgccg
       61 catccggcat gaacaacgcg cactttgtca gtaatctcgg ggcaactaaa tgtcgccccc
      121 tttggttatt accctaccgc tggcgtcggg tcatcgcctt tgtagtcgcg tttgacttcc
      181 gtcacctcat cgcccttctc gttgtgcaga tgcacttcaa gctggttaaa ggcaatgttg
      241 atgtcgtttt cacggcacag ctgatcgata gtacggttca gctcatcgac agtacgacta
      301 cggtcacgca gttcacgcac atacagacgc agctcatgat ccaacgtgct ggcaccaaac
      361 gccgtaaaga agacttccgg cattggttcg tgcatcaccc ttgggtgctc agtcgccgcc
      421 ttcagtaaca ctttacgcac tttttccaga tcggagccat aggccacgcc gagacggatc
      481 accagacgcg tagtagtgtc agtcaacgac cagttgatca gacgctcggt aacaaacgct
      541 ttgttcggga tgatcacttc tttgcgatcg aaatcggtaa tcgttgtcgc acgaatacgg
      601 atcttactta ccgtccccga gaagctacca atggttaccg tatcgccaat acgcaccgga
      661 cgttcgaaca gaatgatcaa accggagacg aagttaccga aaatttcttg taaaccaaaa
      721 ccaagaccta cggataatgc tgcggccagc cactggagtt tatcccaaga gacgcccagc
      781 gatccgaaca ccgtcatcgc accaacggca ataatgatgt agttaaggat ggtagtaatg
      841 gcatacgacg cgccctgacg catattcagt cgcgagagca ccagcacttc cagtaaacca
      901 ggcaggttgc gaatcaacgc ccaggccacc attgaggcga taatcgcaaa caacagactg
      961 cccatggtga cgtttttcac caccgcagcg ccagcttcag tgccgttgta atgccagagc
     1021 gtgatgctgt cgagatagct gaacacggtg atcaaatcgg accaaattgc ccagaacatg
     1081 acaccgaaca gcgcaaacat cagcaacatg gtaatacgca gcgtctgctg gttaacctgt
     1141 tccagtgcaa tggtgggttc ttccggcggt tcggcacctt ctgcgccctc tttcaccaga
     1201 ttctgccgac gcgccaacgc acgacgccag gcgatacgcc gcgccgctac gcttaagccg
     1261 cgcaataccg tctggtagag caagttccag atgatcacca gataaacggt ttcaatccaa
     1321 cgcccggaga ggcgcagcgt ggtgtagaaa taacccgtgg cagtcagcac catcaatgca
     1381 atcgggataa tcgacagcac ggtaatggtg accagtcgca tggtgtgcga ctctttatca
     1441 cgccagcttt cgcggcacat tggccatacc aggaaggcaa tcagcagcag gttgaagaaa
     1501 atcatcgcct gccccagcac atcatccatc agatgcagcg gggaaagttc tgccaccaca
     1561 gaccagaaat ggataggcag caacgcgaga ctgatgcgga caatttgccg acgccagtgg
     1621 ctggtctgct gctccggcat gccgaagtga cgcacggcaa cgccattttt ctccagtacc
     1681 ttccagcaca ggccaaacac cagccagaaa atcgccagtt ttttactgaa cgaccatagc
     1741 agttcgctga tgttgagctg catggtcaac agaatcaggc caaccgcgag aataatcagg
     1801 cacaccggca gcgcgcggat caggtcgata aggatcgctt ttggtgtgtt gagctggcta
     1861 tcgttacgca gggaacccac agcggaagcc agtttctgtt gatacgcttt cagccagccc
     1921 agacgccagt ggatcagccc ggcaatcaac agcagcggca aaccggcgag gaaagcgata
     1981 aaaacggcag gccaggcttt ttcccagttc accgtgattt tcatcgactt aaattcatct
     2041 ttcaggcttt gcgggaacgc tttgatccag tcccagtcca ttggacggtt actgttcacc
     2101 caaaagattt gctgagtcag gatggatttc aggtttttcg atacgctcat taactgctgc
     2161 tggttgattt gcaggttaat ggccatcatc agctggttac ccaactgttt gttgagttga
     2221 tccagcaatt cgcgacgcat atcaaccact tgcaataacg catcgtgaac ttcgctgttg
     2281 acttcgttgg tgtgaccttc ttccagtttg ttgacgaacg catcgctctg gaagagtgca
     2341 tcacgctgct ggttaacttc aaactgttcg agacgcaaat ccgcgatgcg gttggtcatg
     2401 ttttccagtt catccgccga gggcagcgtt tgttgttgct ggtaaaggat acgagacaac
     2461 agcaggctgc ccttcaggac ggcaatctgc tctttaatat tgcgttccga ttgcagcgcc
     2521 cgctccagcc agtttttgac tttaatgttt tgctgcatca actgattacc gttttcagtc
     2581 gcggtaatca gacgctgact taactgctgg ttaatttcca gttcctgttt caccagcgga
     2641 ttagcctgaa tacgcgcggc ttcatccggg gagacggctt cctgcgccgt tttttcagtt
     2701 aaagtcaggc gcttgctgtt taccgcttct tgcaacagct gcaactggtg ctccagacgg
     2761 gcgctgttcg ccgtcacgta atcacgttgc ttttgcaagg tatcctgcaa gacggtgttc
     2821 ccttccaggc ttttacgctg ctggtcaatc tccgcattca gcaacgcctg ctgagcctgc
     2881 attaacactt tctggctggg acgtaaggca gtctcgccga catcagtccc atccagacga
     2941 ctgcgaattt gttgcagctg ctgcgaagcg ttatacatcg cattttgcac gcgttcgggc
     3001 tgcgtctgta acgaaaccag ctggctgtta taagacgcca gatcgttttg tgcgttttgc
     3061 aaatcgtcca gcgcctgggc aacgcgagtt tccagctggc gcaacgaaag cgtactcaga
     3121 attttgcgcg tttcttcgtc gttatcgaca tcgctaagtg ctgttaacgc cgcggtcgcc
     3181 tggcgcattt tttccggcgc ttcagcgact ttttgtcgta gctgaactgt ctcttctttt
     3241 atgcgatcga ttttatcgag ggtggctaat gtatctgtca gatcctgctg caccagtttg
     3301 tcctgagcag aaagatcttt ttgtttattt agtgagtcaa gttgcgcctg caggtccgct
     3361 tttgtcggca gatcaccatt cgatgacgcc cgcgcaaacg ccgtgttctg gcataacaag
     3421 acaaaaacaa aagcaataaa tgctgaaaaa acaaaatgcc gtgatcgttt gtaatactgg
     3481 aacatagtca tgatgaatga aggtttctga acctgaagaa cgacctgaaa aagtcaaacc
     3541 gcaagaatat cacgacgcag tgaaccagaa tagcaacgac gaaaatgtcc aggaaaaatc
     3601 ctggagtcag attcagggtt attcgttagt ggcaggatta cgaagcgtgg ggcacaggag
     3661 atacatctcc agtaagatgg caacgtaatc gcgggcttct tttttaagat caaaagattg
     3721 cggggcaaag agccagtttt ccatcaggcc ggaaatatag ccgcgcataa taattgctgc
     3781 gcgacgcgtc attaaatccg caggcaacat tttcgcttca atacaatgtt ttaacgtttg
     3841 ttctatacgg tcataacttt ccagacagag attacgttgt gcctgttgca caacagccat
     3901 ttctccgaca aattcgcatt tgtggaatat aatctccatc aataatcgac gccgttcttc
     3961 tgtcaccgtg gattcaagaa catgaattaa tatctctctt aatactgaga gtggatcgcc
     4021 agggaatttt acctgatact caagctctag ttcaccaata ttggattctg acagttccca
     4081 gatctcactg aacaaatccg acttgtcttt aaaatgccag tagattgcac cgcgcgtaac
     4141 gccagctgct tttgcaatct cacccagcga ggtggatgat accccctgct gtgagaaaag
     4201 acgtagagcc acatcgagga tgtgttggcg cgtttcttgc gcttcttgtt tggtttttcg
     4261 tgccatatgt tcgtgaattt acaggcgtta gatttacata catttgtgaa tgtatgtacc
     4321 atagcacgac gataatataa acgcagcaat gggtttatta acttttgacc attgaccaat
     4381 ttgaaatcgg acactcgagg tttacatatg aacaaaaaca gagggtttac gcctctggcg
     4441 gtcgttctga tgctctcagg cagcttagcc ctaacaggat gtgacgacaa acaggcccaa
     4501 caaggtggcc agcagatgcc cgccgttggc gtagtaacag tcaaaactga acctctgcag
     4561 atcacaaccg agcttccggg tcgcaccagt gcctaccgga tcgcagaagt tcgtcctcaa
     4621 gttagcggga ttatcctgaa gcgtaatttc aaagaaggta gcgacatcga agctggtgtc
     4681 tctctctatc agattgatcc tgcgacctat caggcgacat acgacagtgc gaaaggtgat
     4741 ctggcgaaag cccaggctgc agccaatatc gcgcaattga cggtgaatcg ttatcagaaa
     4801 ctgctcggta ctcagtacat cagtaagcaa gagtacgatc aggctctggc tgatgcgcaa
     4861 caggcgaatg ctgcggtaac tgcggcgaaa gctgccgttg aaactgcgcg gatcaatctg
     4921 gcttacacca aagtcacctc tccgattagc ggtcgcattg gtaagtcgaa cgtgacggaa
     4981 ggcgcattgg tacagaacgg tcaggcgact gcgctggcaa ccgtgcagca acttgatccg
     5041 atctacgttg atgtgaccca gtccagcaac gacttcctgc gcctgaaaca ggaactggcg
     5101 aatggcacgc tgaaacaaga gaatggcaaa gccaaagtgt cgctgatcac cagtgacggc
     5161 attaagttcc cgcaggacgg tacgctggaa ttctctgacg ttaccgttga tcagaccact
     5221 gggtctatca ccctacgcgc tatcttcccg aacccggatc acactctgct gccgggtatg
     5281 ttcgtgcgcg cacgtctgga agaagggctt aatccaaacg ctattttagt cccgcaacag
     5341 ggcgtaaccc gtacgccgcg tggtgatgcc accgtactgg tagttggcgc ggatgacaaa
     5401 gtggaaaccc gtccgatcgt tgcaagccag gctattggcg ataagtggct ggtgacagaa
     5461 ggtctgaaag caggcgatcg cgtagtaata agtgggctgc agaaagtgcg tcctggtgtc
     5521 caggtaaaag cacaagaagt taccgctgat aataaccagc aagccgcaag cggtgctcag
     5581 cctgaacagt ccaagtctta acttaaacag gagccgttaa gacatgccta atttctttat
     5641 cgatcgcccg atttttgcgt gggtgatcgc cattatcatc atgttggcag gggggctggc
     5701 gatcctcaaa ctgccggtgg cgcaatatcc tacgattgca ccgccggcag taacgatctc
     5761 cgcctcctac cccggcgctg atgcgaaaac agtgcaggac acggtgacac aggttatcga
     5821 acagaatatg aacggtatcg ataacctgat gtacatgtcc tctaacagtg actccacggg
     5881 taccgtgcag atcaccctga cctttgagtc tggtactgat gcggatatcg cgcaggttca
     5941 ggtgcagaac aaactgcagc tggcgatgcc gttgctgccg caagaagttc agcagcaagg
     6001 ggtgagcgtt gagaaatcat ccagcagctt cctgatggtt gtcggcgtta tcaacaccga
     6061 tggcaccatg acgcaggagg atatctctga ctacgtagcg gcgaatatga aagatgccat
     6121 cagccgtacg tcgggcgtgg gtgatgttca gttgttcggt tcacagtacg cgatgcgtat
     6181 ctggatgaac ccgaatgagc tgaacaaatt ccagctaacg ccggttgatg tcattaccgc
     6241 catcaaagcg cagaacgccc aggttgcggc gggtcagctc ggtggtacgc cgccggtgaa
     6301 aggccaacag cttaacgcct ctattattgc tcagacgcgt ctgacctcta ctgaagagtt
     6361 cggcaaaatc ctgctgaaag tgaatcagga tggttcccgc gtactgctgc gtgatgtggc
     6421 gaagattgag ctgggtggtg agaactacga catcatcgca gagtttaacg gccaaccggc
     6481 ttccggtctg gggatcaagc tggcgaccgg tgcaaacgcg ctggataccg ctgcggcaat
     6541 ccgtgctgaa ctggcgaaga tggaaccgtt cttcccgtcg ggtctgaaaa ttgtttaccc
     6601 gtacgacacc acgccgttcg tgaaaatctc tattcacgaa gtggttaaaa cgctggtcga
     6661 agcgatcatc ctcgtgttcc tggttatgta tctgttcctg cagaacttcc gcgcgacgtt
     6721 gattccgacc attgccgtac cggtggtatt gctcgggacc tttgccgtcc ttgccgcctt
     6781 tggcttctcg ataaacacgc taacaatgtt cgggatggtg ctcgccatcg gcttgttggt
     6841 ggatgacgct atcgttgtgg tagaaaacgt ggagcgtgtt atggcggaag aaggtttgcc
     6901 gccaaaagaa gctacccgta agtcgatggg gcagattcag ggcgctctgg tcggtatcgc
     6961 gatggtactg tcggcggtat tcgtaccgat ggccttcttt ggcggttcta ctggtgctat
     7021 ctatcgtcag ttctctatta ccattgtttc agcaatggcg ctgtcggtac tggtagcgtt
     7081 gatcctgact ccggctcttt gtgccaccat gctgaaaccg attgccaaag gcgatcacgg
     7141 ggaaggtaaa aaaggcttct tcggctggtt taaccgcatg ttcgagaaga gcacgcacca
     7201 ctacaccgac agcgtaggcg gtattctgcg cagtacgggg cgttacctgg tgctgtatct
     7261 gatcatcgtg gtcggcatgg cctatctgtt cgtgcgtctg ccaagctcct tcttgccaga
     7321 tgaggaccag ggcgtattta tgaccatggt tcagctgcca gcaggtgcaa cgcaggaacg
     7381 tacgcagaaa gtgctcaatg aggtaacgca ttactatctg accaaagaaa agaacaacgt
     7441 tgagtcggtg ttcgccgtta acggcttcgg ctttgcggga cgtggtcaga ataccggtat
     7501 tgcgttcgtt tccttgaagg actgggccga tcgtccgggc gaagaaaaca aagttgaagc
     7561 gattaccatg cgtgcaacac gcgctttctc gcaaatcaaa gatgcgatgg ttttcgcctt
     7621 taacctgccc gcaatcgtgg aactgggtac tgcaaccggc tttgactttg agctgattga
     7681 ccaggctggc cttggtcacg aaaaactgac tcaggcgcgt aaccagttgc ttgcagaagc
     7741 agcgaagcac cctgatatgt tgaccagcgt acgtccaaac ggtctggaag ataccccgca
     7801 gtttaagatt gatatcgacc gggaaaaagc gcaggcgctg ggtgtttcta tcaatgacat
     7861 taacaccact ctgggcgctg catggggcgg tagctatgtg aacgacttta tcgaccgcgg
     7921 tcgtgtgaag aaagtttacg tcatgtcaga agcgaaatac cgtatgctgc cggatgatat
     7981 cggcgactgg tatgttcgtg ctgctgatgg tcagatggtg ccgttctcgg cattctcctc
     8041 ttctcgttgg gagtacggtt cgccgcgtct ggaacgttac aatggcctgc catccatgga
     8101 aatcttaggc caggcggcac cgggtaaaag taccggtgaa gcaatggagc tgatggaaca
     8161 actggcgagc aaactgccta ccggtgttgg ctatgactgg acggggatgt cctatcagga
     8221 acgcctctcc ggcaaccagg caccttcact gtacgcgatt tcgttgattg tcgtattcct
     8281 gtgtctggcg gcgctgtacg agagctggtc gattccgttc tccgttatgc tggtcgttcc
     8341 gctgggggtt atcggtgcgt tgctggctgc caccttccgt ggcctgacca atgacgttta
     8401 cttccaggta ggcctgctca caaccattgg gttgtcggcg aagaacgcga tacttatcgt
     8461 cgaattcgcc aaagacttga tggataaaga aggtaaaggt ctgattgaag cgacgcttga
     8521 tgcagtgcgg atgcgtttac gtccgatcct gatgacctcg ctggcgttta tcctcggcgt
     8581 tatgccgctg gttatcagta ctggtgctgg ttccggcgcg cagaacgcag taggtaccgg
     8641 tgtaatgggc gggatggtga ccgcaacggt actggcaatc ttcttcgttc cggtattctt
     8701 tgtggtggtt cgccgccgct ttagccgcaa gaatgaagat atcgagcaca gccatactgt
     8761 cgatcatcat tgatacaacg tgtaatcact aaggccgcgc aagcggcctt ttttatgcat
     8821 aacctacgaa cattaaggag taattgaacc accaactc
//
LOCUS       DAZGJW010000174         8852 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZGJW010000174 DAZGJW010000000
VERSION     DAZGJW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.174"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(17..643)
                     /gene="upp"
                     /locus_tag="ACHKIK_004346"
     CDS             complement(17..643)
                     /gene="upp"
                     /locus_tag="ACHKIK_004346"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO9085398.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            968..2005
                     /gene="purM"
                     /locus_tag="ACHKIK_004347"
     CDS             968..2005
                     /gene="purM"
                     /locus_tag="ACHKIK_004347"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO9085399.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTQLDGKPL
                     ADYLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            2005..2643
                     /gene="purN"
                     /locus_tag="ACHKIK_004348"
     CDS             2005..2643
                     /gene="purN"
                     /locus_tag="ACHKIK_004348"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO9085400.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            2814..4880
                     /gene="ppk1"
                     /locus_tag="ACHKIK_004349"
     CDS             2814..4880
                     /gene="ppk1"
                     /locus_tag="ACHKIK_004349"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO9085401.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANVQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            4885..6426
                     /gene="ppx"
                     /locus_tag="ACHKIK_004350"
     CDS             4885..6426
                     /gene="ppx"
                     /locus_tag="ACHKIK_004350"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO9085402.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(6465..8708)
                     /gene="pdeF"
                     /locus_tag="ACHKIK_004351"
     CDS             complement(6465..8708)
                     /gene="pdeF"
                     /locus_tag="ACHKIK_004351"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO9085403.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
BASE COUNT         2295 a         2172 c         2185 g         2200 t
ORIGIN      
        1 taattatttt tattctttat ttcgtaccaa agattttgtc accggcatcg ccgaggcccg
       61 gaataatgta tccgtgctcg ttcagtccct gatcaatcga tgcggtatac agttcgacgt
      121 ccgggtgcgc tttttccagc gcagcgatac cttctggcgc agctaccagc accagaactt
      181 tgatgctgct gcagcccgct tttttcagca ggtcgatggt cgcgataacg gaaccaccgg
      241 ttgccagcat tgggtcaacg atcagcgcca tacgctcatc gatgttagaa accagtttct
      301 ggaagtacgg taccggctcc agcgtttctt cattacggta cataccgaca acgctgatgc
      361 gcgcgctcgg aacattttcc agcacaccgt ccatcatccc cagaccggca cgcagaattg
      421 gcacaacggt aattttctta cctttgatct ggtcgatttc taccgggccg ttccagcctt
      481 cgatagttac tttttccgtt tcgaggtcgg cggtcgcttc gtaagtcagc aggctaccca
      541 cttcggaagc gagttcgcga aagcgcttgg tgctgatatc ttgctcacgc atcagtccca
      601 gcttgtgttt gacgagtggg tgtttgactt ccacgatctt catactcttt ctcctttgag
      661 gggcagccac aaaaaaaatc gacggattat acctcctttc ttcaaggcgg caatattctt
      721 ttcgttgact ttagtcaaaa tgataacggc ttgagataaa gttattttat attcaaatgg
      781 ttatgaaaga agattattcc atccgaaaac taacctttac cctggtacaa gtcttctttc
      841 gccgcgcgcc tgggcaaaag acgtgcaaaa aggttgtgta aagcagtctc gcaaacgttt
      901 gctttccctg ttagaattgc gccgaatttt atttttctac cgcaagtaac gcgtggggac
      961 ccaagcagtg accgataaaa cctctcttag ctacaaagat gccggtgttg atattgacgc
     1021 gggtaatgct ctggttggaa gaatcaaagg cgtagtgaag aaaacgcgtc gtccggaagt
     1081 gatgggcggt ctgggcggct tcggtgcgct gtgtgcattg ccgcaaaaat atcgtgaacc
     1141 cgtgctggtt tccggcactg acggcgtagg taccaagctg cgtctggcaa tggacttaaa
     1201 acgtcacgac accattggta ttgatctggt cgccatgtgc gttaatgacc tggtggtgca
     1261 aggtgcagag ccgctgtttt tcctcgacta ttacgcaacc ggaaaactgg atgttgatac
     1321 cgcttcagcg gtgatcagcg gtatcgcgga aggatgtctg caatcgggct gttcactggt
     1381 gggtggcgaa acggcagaaa tgccggggat gtatcacggt gaggattacg atgtcgcggg
     1441 tttctgcgtt ggcgtggtag aaaaatcaga aatcatcgac ggctctaaag tcagcgacgg
     1501 cgatgtgctg attgcactcg gttccagcgg tccgcactcg aacggctatt cgctggtgcg
     1561 caaaattctt gaagtcagcg gttgtgatcc gcaaaccacc caacttgatg gtaagccatt
     1621 agccgattat ctgctggcac cgacccgcat ttacgtgaag tcagtgctgg agttgattga
     1681 aaaggtcgat gtgcatgcca ttgcgcacct gaccggcggc ggcttctggg aaaacattcc
     1741 acgcgtattg ccagataata ctcaggcagt gattgatgaa tcttcctggc agtggccgga
     1801 agtgttcaac tggctgcaaa cggcaggtaa cgttgagcgc catgaaatgt atcgcacctt
     1861 caactgcggc gtcgggatga ttattgccct gcctgctccg gaagtggaca aagccctcgc
     1921 cctgctcaat gccaacggtg aaaacgcgtg gaaaatcggt atcatcaaag cctctgattc
     1981 cgaacaacgc gtggttatcg aataatgaat attgtggtgc ttatttccgg taacggaagt
     2041 aatttacagg caattattga cgcctgtaaa accaacaaaa ttaaaggcac cgtacgggca
     2101 gttttcagca ataaggccga cgcgttcggc cttgaacgcg cccgccaggc gggtattgca
     2161 acgcatacgc tcatcgccag cgcgtttgac agtcgtgaag cctatgaccg ggagttgatt
     2221 catgaaatcg acatgtacgc acccgatgtg gtcgtgctgg ctggttttat gcgcattctc
     2281 agcccggcgt ttgtctccca ctatgccggg cgtttgctga acattcaccc ttctctgctg
     2341 ccgaaatatc ccggattaca cacccatcgt caagcgctgg aaaatggtga tgaagagcac
     2401 ggtacatcgg tgcatttcgt caccgatgaa ctggacggtg gcccggttat tttacaggcg
     2461 aaagtcccgg tatttgctgg tgatacggaa gatgacatca ccgcccgcgt gcaaacccag
     2521 gaacacgcta tttatccact ggtgattagc tggtttgccg atggtcgtct gaaaatgcac
     2581 gaaaacgccg cgtggctgga tggtcaacgt ctgccgccgc agggctacgc tgccgacgag
     2641 taatgccccg tagttaaagc gccagctctg ccgctggcgt ttttcaattc acctgtaaat
     2701 cgcaagctcc agcagttttt ttcccccttt tctggcatag ttggacatct gccaatattg
     2761 ctcgccataa tatccaggca gtgtcccgtg aataaaacgg agtaaaagtg gtaatgggtc
     2821 aggaaaagct atacatcgaa aaagagctca gttggttatc gttcaatgaa cgcgtgcttc
     2881 aggaagcggc ggacaaatct aacccgctga ttgaaaggat gcgtttcctg gggatctatt
     2941 ccaataacct tgatgagttc tataaagtcc gcttcgctga actgaagcga cgcatcatta
     3001 ttagcgaaga acaaggctcc aactctcatt cccgccattt actgggcaaa attcagtccc
     3061 gggtgctgaa agccgatcag gaattcgacg gcctctacaa cgagctattg ctggagatgg
     3121 cgcgcaacca gatcttcctg attaatgaac gccagctctc cgtcaatcaa caaaactggc
     3181 ttcgtcatta ttttaagcag tatctgcgtc agcacattac gccgatttta atcaatcctg
     3241 acactgactt agtgcagttc ctgaaggatg attacaccta tctggcggtg gaaattatcc
     3301 gtggtgatac catccgttac gcgctgctgg agatcccatc agataaagtg ccgcgctttg
     3361 tgaatttacc gccagaagcg ccgcgtcgac gcaagccgat gattcttctg gataacattc
     3421 tgcgttactg ccttgatgat attttcaaag gcttctttga ttatgacgcg ctgaatgcct
     3481 attcaatgaa gatgacacgc gatgccgaat acgatttagt gcatgagatg gaagccagcc
     3541 tgatggagtt gatgtcttcc agtctcaagc agcgtttaac tgctgagccg gtgcgttttg
     3601 tttatcagcg cgatatgccc aatgcgctgg ttgaagtgtt acgcgaaaaa ctgactattt
     3661 cccgctacga ctccatcgtc cccggcggtc gttatcataa ttttaaagac tttattaatt
     3721 tccccaatgt cggcaaagcc aatctggtga acaaaccact gccgcgttta cgccacattt
     3781 ggtttgataa agcccagttc cgcaatggtt ttgatgccat tcgcgaacgc gatgtgttgc
     3841 tctattatcc ttatcacacc tttgagcatg tgctggaact gctgcgtcag gcttcgttcg
     3901 acccgagcgt actggcgatt aaaattaaca tttaccgcgt ggcgaaagat tcacgcatca
     3961 tcgactcgat gatccacgcc gcacataacg gtaagaaagt cactgtggtg gttgagttac
     4021 aggcgcgttt cgacgaagaa gccaacattc actgggcgaa gcgcctgacc gaagcaggcg
     4081 tgcacgttat cttctctgcg ccggggctga aaattcacgc caaactgttc ctgatttcac
     4141 gtaaagaaaa cggtgaagtg gtccgttacg cacacatcgg gaccgggaac tttaacgaaa
     4201 aaaccgcgcg tctttatact gactattcgt tgctgaccgc agatgcgcgt atcaccaacg
     4261 aagtacggcg ggtatttaac tttattgaaa acccataccg cccagtgaca tttgattatt
     4321 taatggtgtc accgcaaaac tctcgccgtc tgttatatga aatggtagac cgcgaaatcg
     4381 ccaacgtgca gcaagggctg cccagtggta tcaccctgaa gctaaataac cttgtcgata
     4441 aaggcctggt tgatcgtctg tatgcggcct ccagctccgg cgtaccggtt aatctgctgg
     4501 ttcgcggaat gtgttcgctg atccccaatc tggaaggcat tagcgacaac attcgtgcca
     4561 tcagtattgt tgaccgttac cttgaacatg accgggttta tatttttgaa aatggcggcg
     4621 ataaaaaggt ctacctttct tccgccgact ggatgacgcg caatattgat tatcgtattg
     4681 aagtggcgac accgctgctc gatccgcgcc tgaagcagcg ggtgctggac atcatcgaca
     4741 tattgttcag cgatacggtc aaagcacgtt atatcgataa agaactcagt aatcgctacg
     4801 ttccccgcgg caatcgccgc aaagtacggg cgcagttggc gatttatgac tacatcaaat
     4861 cactcgaaca acctgaataa ccctatgcca atacacgata aatcccctcg tccgcaggag
     4921 tttgctgcgg tcgatcttgg ttcaaacagt tttcacatgg tcatagcccg tgtggtagat
     4981 ggtgccatgc agattattgg ccgcctgaaa cagcgggtgc atctggcgga cggcctgggg
     5041 ccagataata tgttgagtga agaggcaatg acgcgcggtt tgaactgtct gtcgctgttt
     5101 gccgaacggc tacaagggtt ttctcctgcc agcgtctgta tagttggcac ccatacactg
     5161 cgtcaggcgc tgaacgccac tgactttctg aaacgcgcgg aaaaggtcat tccctacccg
     5221 attgaaatta tttccggtaa tgaagaagcc cgtctgattt ttatgggcgt ggaacatacc
     5281 caaccggaaa aaggtcgcaa actggttatt gatattggcg gcggatctac ggaactggtg
     5341 attggtgaaa atttcgaacc tattctcgtt gaaagccgcc ggatgggttg tgtcagcttt
     5401 gcccagcttt atttccctgg cggggtcatc aataaagaga attttcagcg cgctcgcatg
     5461 gcggcagcac aaaaactgga aactttaacc tggcaattcc gtattcaggg ctggaacgtg
     5521 gcaatgggcg cttccggtac cataaaagcc gcccatgaag tattaatgga aatgggcgag
     5581 aaagacggga taattacccc ggaacgtctg gaaaaactgg taaaagaagt tttacgtcac
     5641 cgtaatttcg catcgctgag tttaccgggt ctttccgaag agcggaaaac agtcttcgtt
     5701 cctggactgg cgattttatg tggtgtgttt gatgctttag ccatccgtga actgcgcctt
     5761 tctgacgggg cgttacgcga aggcgtactg tatgaaatgg aaggacgttt ccgtcatcag
     5821 gatgtgcgta gtcgcaccgc cagcagcctc gccaaccagt atcacatcga cagcgaacag
     5881 gcccgacgag tgctggatac cactatgcaa atgtacgaac agtggcggga acagcaaccg
     5941 aagctggcgc atccgcaact ggaggcgcta ctgcgatggg ccgccatgct gcatgaggtc
     6001 gggttgaata tcaaccacag cggtttgcat cgccactccg cttatattct gcaaaacagt
     6061 gacttgccgg gttttaatca ggaacagcag ctgatgatgg cgacactggt gcgctatcac
     6121 cgtaaagcga ttaagctcga cgatctgccg cgctttacct tgtttaagaa gaaacagttc
     6181 ctgccactga tacagctatt gcgccttggc gtattactaa acaatcaacg tcaggcaacc
     6241 accacaccgc caacattgac actgattact gatgacagtc actggacact gcgtttcccg
     6301 catgactggt ttagtcagaa tgcgctggta ctgcttgatc tggaaaagga gcaagaatac
     6361 tgggaaggcg tggctggctg gcggttgaaa attgaagaag aaagtacacc tgaaatcgcc
     6421 gcttaatgct ttgcgggccg acgagaaatg tcggcccgca ttattcaggc actttcgcga
     6481 atgggttcga tttcattcag cgtatcaatt aacggttgcg gcttaccaat aagataaccc
     6541 tgcatataat cgatccccaa agagagcacc gcctcgcgga tctcttcgtt ttcaacgtac
     6601 tctgccacta cccgcatttt cttcattcgc gccaggtggc aaatcgatgc cactatctga
     6661 taatccagac tatttgacac aatattgcgg ataaaactgc cgtcaatttt aagcagatcg
     6721 gcattcacat ttttaagccg cgcatagctg gcgtagccgg tgccgaaatc atcaatcgca
     6781 atctggcagc ctaattcctg aagatgctgc aaggtgattt gcgcctgctt aacattggtc
     6841 agagcattac tttcggtgac ttcaaaaata agttgccacg cttcaatctg atatttatcc
     6901 agcaactgac tgacttcaac aggaaaacga gcctgacata ccgaggttgg agacagatta
     6961 atagcaaaac ggtgagcggg catcttcgct ctgttttcag ccataaattg cagcgtatgc
     7021 tcaatgaccc acatgtcgat actcgacgat aaaccaaatt cgtgcgcgac cggcaagaag
     7081 ctatcggggc cgatcagttc atcattctca cctttcatgc gcagaagaat ttcatggtaa
     7141 acatcaccac gcataccggt aatcggctgg gccatcagga aaaaatggtt gtgttccagc
     7201 gcctgctgta gtcgattcat catcgcgact ttatctttca attcgcgttg caaatacatt
     7261 gccccgcgac gctgcatatt ttccggggcg ttggtcacga tggaaagttc ggcgaccgta
     7321 tttagctctc ccagcagcag gtagatatga ttcactggcg agcgcacata gcagtaactg
     7381 acgccaatct gcggttgcat cggcatgcca tcccaaaaga aacgaaattg cttgagatgg
     7441 ctatccagtg cggtaatgcg ctcctggtgc gattctgtat tcagccgcag cgcgagatcg
     7501 ttacccgaaa gctgataaac atcttcaccc ggttccagca agggtgacag ccagtgagaa
     7561 agtttttgct tgtattgaat ccgcagcatg atgccatagt tcttaaccag catttccatg
     7621 ccagggatgc gtaaataaca aagcgcagac cagggggcat cacgcaacgc gcgattcagg
     7681 gcgcgaacat ttggcagatg aaccaccggg tccacatacg caagccgctg aatgcgtctg
     7741 actaccgctc gctgacgagt tgccagtaca gccatataat tgacaataaa agagaatacc
     7801 agataactgg aggaggttat ggtcagctgc gtggtatagc caggataaat gggaatgtaa
     7861 ttttgatagc tgtggatgct gatcatcaac acgaccgccc agagcagcga aatcagctta
     7921 taaccatagc gcatcgctcc ccacatcatc aggggcagca ataatgacaa ggtataattg
     7981 gtgctaaaaa ttgtgctttt ttcatttaac ggcatgcata acagcaacaa taaagcacct
     8041 aatgccagta gccagagcgc gaactctttt ttggtgactt tggcatcaac ctgctgtttt
     8101 aattgcgaat aatagctacg taaataaaat ggatttcgca ctacccgaat gatgaagtag
     8161 cacagcggga caccgatcag attacccacc agtaaggcct gataattgat taaagtcccg
     8221 aggttaaaag gcatgacacc gaccagattt tctctgctcg ccagtaatcc tacaaatgca
     8281 gcaaactgga aaagtatcag aaacagcgtt gcaggaaaca caatctgcca gaatatacgc
     8341 tgggaaatta aacgggtatc accatgtgaa acgttgttac gccggggagt aaagaccctg
     8401 taaccgcccc aacagagaat gataatgata ataaaattag ccgttaatga gagcgtttcg
     8461 taaaaaccca gctctgcata tttgcggaca aaaatcccta aggtgatccc tggcaatgcc
     8521 gcccagctga aaaacatcat catgctgatc atcagtgcca aaggcagata aaagagaaaa
     8581 acctcaccgg aagaaatatg cgcgaaagta ttaatgtggg cgaaaatggg caagattaaa
     8641 gaaggcagga acagcggaag cccccaccat ttatcacgta tttttatata agttgcattc
     8701 agtttcatag atgctcagca gaatccccca catcctgaag gaggtgtatt cagacaggca
     8761 tcccacctga cttcgaatga tgattattca tcactataga gagcattgat tctaagtgtc
     8821 atatgaaagt tccaattgat atatatcaaa ca
//
LOCUS       DAZGJW010000175         8773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZGJW010000175 DAZGJW010000000
VERSION     DAZGJW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.175"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(219..554)
                     /locus_tag="ACHKIK_004352"
                     /pseudo
     CDS             complement(219..554)
                     /locus_tag="ACHKIK_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            484..813
                     /locus_tag="ACHKIK_004353"
     CDS             484..813
                     /locus_tag="ACHKIK_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO9085404.1"
                     /translation="MLDTQELAPVAIALLLSVIGGIGTFLMDVRDGRQSGNLLGLVTE
                     IFVAVTAGAVAYLLGQHEGWELSITYLMVTIASNNGHEVISGMKRVNIDSILNVLTSL
                     VKKGGGK"
     gene            810..1253
                     /locus_tag="ACHKIK_004354"
     CDS             810..1253
                     /locus_tag="ACHKIK_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO9085405.1"
                     /translation="MIGWGVCALALALADRYLLKRKDITHLELGDVEIKPGFIRVPFK
                     YRSKFPFLRGATVRYWIRDVQKPTTVIEGEQRCLTSAEQGENSEWLYIPTEYMGKGER
                     LWHFNVMVTHGDSFINPLYRIFPVTQQIRRSYVINLAQDVSDDEK"
     gene            1240..1842
                     /locus_tag="ACHKIK_004355"
     CDS             1240..1842
                     /locus_tag="ACHKIK_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="odaE"
                     /protein_id="HFO9085406.1"
                     /translation="MTKNKYATVDFDQVNEKGLKSLIAAINKTGVTVIEVDSSNRATT
                     KDGVKVKTAKLVLNDGQILAIQVNDTGDISSVRLNGKAIPNAQSPDIKTLGTVMGQAA
                     RKNSAKFQKSLIAKAKRVANPVDKKPAVKSNFQRLQEAKQRNAQVVAAYKSAQNSVSF
                     NQQQITDLRAKLDKETGRLNNEKARNGELKRRLKQLKAGN"
     gene            1844..3763
                     /locus_tag="ACHKIK_004356"
     CDS             1844..3763
                     /locus_tag="ACHKIK_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085407.1"
                     /translation="MEQFNINKGVAIKPGLDVLPPPVTDDEYRALMAGEDRYLMTESN
                     TLEEIEATFFYDTPIHWCATDLLEAISSTRLQLHRTMQAFVRALNQKLNGTGISAGSD
                     KTGDVAQNGARAIGGAEIGRARNVNGLPVLPAIIPLSDGQTISILFHSPTAENRITNS
                     DTLIAFQFLLNKKDVTHTVAPMSGRDMTLVQVTMKLANLAEKNSAKFQRAQKKKKALV
                     DEITQLQADSDQKEDAMSDLADQVAAVEGQKADLEQKINVVASEADSLYQENERLQAE
                     IDQLNRTGGRDTIAPAGMTGGHSRALTDRLASIKNRMHMDGEATLSNGASMKQFIGDG
                     EGYIQLTDPDGSVYMIKAKSIQGVDMAEAIGKLFKAYKAGNVSEYLVQPEEHKPENVE
                     PEPAEDTGSSSPEPEVSVGAYRYALQMRPAAPGAIPEGNKAILPRPDEGDPYYEYARY
                     GIATYDTPLSDQQMSEYDLKLLPREDSFDFLAKTLTNGPFGKYAQKALELATSSPDEF
                     RVMLKTQFQKTFPNIAFPGGAGTEKMVQSMINALQAEVGEITQPEPAPAQPDETVSEA
                     DAEANKAIEYLNNVMDMQSTDMAEIRNARGNVREAIAALQAAGRFEENEELVNGAARH
                     LADLLVAIQKAGVAA"
     gene            3760..4125
                     /locus_tag="ACHKIK_004357"
     CDS             3760..4125
                     /locus_tag="ACHKIK_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ddrA"
                     /protein_id="HFO9085408.1"
                     /translation="MTLSAIELMDLSDKLDALMSKAATASGMELLDISDEIDQIMQQM
                     GYGASGGGSGEEKQPSEHDGVPKLVADFLADKFVDQSTDAFIGTLQDLSQYVGTYIDL
                     DQVKQHTAAWIAANIEEAA"
     gene            4138..7125
                     /locus_tag="ACHKIK_004358"
     CDS             4138..7125
                     /locus_tag="ACHKIK_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085409.1"
                     /translation="MSLSDQVVMATSIETLIELLKNLPDYGRVSYVVTAKGDEVKTAF
                     DIVDASALLVSNTLDGKINPDYPQELQPRDRTRASSLLQVNQISKDLRPAQLTDSGLS
                     SHGAPIIGEDNAVESGNGRTMGIIKAYQDGNADRYREYLIEHATEFGIRPEKVESMTA
                     PVLVRRRLTKVDRVQFAKDSNISDLQEMAASEKAFVDADNITPAMMALFNPSESGDLL
                     SRSNDAFIRGFMTQVGATQAAGLVTEDGRPTRQLVDRIQNAIFAKAYKDARLVRMVAE
                     EPDPDMRNVLTALNAAANDFVQMQALSGEAHKQAVTTIVDGIETADSLDKKALAALKD
                     AVDLVRQSKESGQHITDVIAQGDMFSETAPEVKALALFIVANNRSAKRMATAFKLMAQ
                     RINDELQHQGQALGDMFGGGDVSLQDILRQVSQELENEGMQGISGGLFESASGGSYNG
                     VAPYTSLLLHRASGIKDIIHLIRLLSRTDPQDEQLVQVLAHFVRMPVADVKKWCRLFG
                     ISNSLLRGLLNHASSLGRDGFDEIAQAIKNGDMPPAIDWFSIRPTRVKAFLSAAHSAS
                     PLAEMVQRLSLIFTDHTALGDLTLDEMKEASIQWADQQNEVNSDFLPAFRKAVSKADD
                     ARGILKAFKALQSRVNKHVGDIDGVTAEGRDILKEHGITPEFIDEIRTDMQREVVSSL
                     QIVARALADANPKSAAIVNRVIGDIEASEGMGALKLFLSRAFNPNGNILPGIIGEAKK
                     YVSEEELEQLDQLLKRFSYNPQTRWQMNQRSMGSVHEKVLSAMNSAIANSSVSEEKAL
                     EWADSFITEEVEEARAGQNGGIDLRKELADIYRLTGGKISTLSKVVHHQGRAYANLNG
                     VVAVNLNDENASALWHELGHHLEYSNPGLLEKARSFLKANVEGDKPSFVNIGGRGKPE
                     WCFRSRLSNIYMAKVYPPASVSNTGKIRQKSPTISKTSATEVFSMALQLYHDKEAAAA
                     SLMNGDGLLELLLGVAKELNNAD"
     gene            7115..7423
                     /locus_tag="ACHKIK_004359"
     CDS             7115..7423
                     /locus_tag="ACHKIK_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085410.1"
                     /translation="MQIKIAAPLGGDAIIEFDDNEEVSGRLSIISGDITEDMIAEAIA
                     GANPNSYMGFVNTLDAPASDVLRTLHLYAGWFVDWPAVEGGDEDDDDDFGDHVDQIVY
                     "
     gene            complement(7455..8759)
                     /gene="yjjJ"
                     /locus_tag="ACHKIK_004360"
     CDS             complement(7455..8759)
                     /gene="yjjJ"
                     /locus_tag="ACHKIK_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014715731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family toxin
                     YjjJ"
                     /protein_id="HFO9085411.1"
                     /translation="MATHATTIRSLLNQGPMTPRQLVEKLSVSQPTISRALRELGDEI
                     VRIGNGRSIQYALRDSFRGFESVPIYRITEEGCICVLGTLIPVRPEGYVMVEDGKNNR
                     HSDGLPWWLFDMRPQGFLGRAYASAYALELNLPSNPEQWSDTDVIKALLKHGHDVVGN
                     LLIGEQARTHFLEMQPPVPVNREIEYPVLALAAGEGDVPGSSAGGEQPKFCTFTERGH
                     VLVKFSAPNDTPISERWRDLLFAEHLALKVLGVETEVFDYNGQRFLEIPRFDRVGQLG
                     RVGVFSLRALDAEFVGNARATWPLLVKELVKNKCVHPDAIIIATRLWSFGVLIGNTDM
                     HNGNLSFISSHGRPYHIAPAYDMLPMGFAPRSGGAMSNELRKTALPDFVSRKIWDESL
                     ELAEKFYALACEADKFSANFTPCLEALRIHLDDCKIRIARLA"
BASE COUNT         2382 a         1947 c         2281 g         2163 t
ORIGIN      
        1 tttatggctt gccatttcta tacatatctg ttaaaaattt tgacttgttt gttgaaaata
       61 gatttaattg attggtttat tttttacatg acgctcttta atatagcttc aggtatatat
      121 aagcacaatg cattatttcc agaggaaatt aacaaaattg ttagctctta tttgttgagc
      181 ttatagatat taattaaggc ccgattccgg gcctttcctc attccggctt ttcgggaaac
      241 gttactggaa ggatggaggt atccgtggat tcgactttct gcgcatagag aatccactcg
      301 gttaattttt gtttaccctg aacggaaata atgcctagcc gtaactgtga gtcccagagc
      361 tgagttttat ccctgacgag ctgtagcagg ctttgctttt ctttttccgc ttgttgtctt
      421 tgcatgaccg gttgagaagt tactttgcat accattacct cctgacaacg taggagggaa
      481 cttgtgcttg acacacagga attagctcca gttgctattg cgctcctgct ttcagtaatt
      541 ggtgggatag gcacgttcct gatggatgtc cgagacggtc gccagtctgg caatttgttg
      601 ggattggtta cggagatctt tgttgcagtg acagctggcg cggtggcgta cctattgggg
      661 caacacgagg gctgggagtt atcaattacg tacttaatgg taacgatagc cagcaataac
      721 ggtcatgagg tgatttcagg gatgaaacga gtgaatatcg atagcattct gaatgttctt
      781 acaagtttgg tgaaaaaggg aggtgggaaa tgattggctg gggtgtatgc gctcttgcgt
      841 tagccttagc cgatcgctat ttgctaaaac gcaaggacat cacgcattta gaacttggtg
      901 atgtggaaat taaaccgggt ttcatccggg tgccgttcaa ataccggtct aaattcccgt
      961 ttttgcgcgg cgcaacggtc agatattgga tccgcgatgt tcagaagcca acgacagtga
     1021 ttgaaggcga acaacgttgt ctgacgtcgg ctgaacaggg cgaaaacagt gaatggttgt
     1081 acatacccac tgaatatatg ggtaaaggag aacggctgtg gcatttcaac gtcatggtta
     1141 cgcatggcga ctcgttcatt aacccgttgt atcggatttt ccctgttact cagcaaatcc
     1201 gcagaagtta cgtaataaat ctcgcacagg atgtgtcaga tgacgaaaaa taagtatgca
     1261 acggtcgatt ttgaccaggt taatgaaaag gggctgaaat cccttatcgc ggcgatcaat
     1321 aaaaccggtg ttacggtaat tgaggttgac tccagcaacc gcgcaacaac gaaagatggc
     1381 gttaaagtta aaaccgcaaa gctggttctt aacgacggac aaattcttgc catacaggta
     1441 aacgatactg gcgatatatc gtctgtgagg ctgaatggaa aagctattcc taacgctcag
     1501 tcgccggata tcaagacgct tggtaccgtc atggggcaag cggcccgcaa aaactccgca
     1561 aaattccaga aatcactgat cgccaaagcg aaacgtgttg ccaatccggt agacaagaaa
     1621 ccggcagtaa aatccaactt tcagcgcctg caagaggcaa aacagcggaa tgctcaggtg
     1681 gttgccgctt ataaatcagc gcagaactcg gtgtctttca atcaacagca gatcactgat
     1741 ttgcgggcga agctggataa ggagacaggc cgactcaata acgaaaaggc ccgaaatggc
     1801 gaactcaaac gccgtcttaa gcaactgaaa gcaggaaatt aacatggaac agttcaatat
     1861 caataaaggg gtggcgatca agcctgggct tgacgtgctt cccccgccag tgactgatga
     1921 tgaatatcgc gcattaatgg ccggtgagga ccgctatctg atgacggaat ccaacaccct
     1981 ggaggaaatc gaggctacgt tcttctatga cacgccgatc cactggtgtg ctacggattt
     2041 actggaggcg attagttcta ctcgtttgca gctacaccgg accatgcagg catttgtccg
     2101 ggcattgaac cagaagctga atggtaccgg aatctctgcg gggagtgata aaacggggga
     2161 cgtggcccag aacggtgcac gagcgatcgg cggggctgaa attggccggg cgcgtaacgt
     2221 taacgggctg ccggttctgc cagccattat tccgctcagt gatggtcaga ctatcagcat
     2281 tctgtttcat agcccgacag cggaaaaccg gatcaccaat agcgatacgc tgattgcttt
     2341 ccagttctta ctgaataaaa aagacgttac tcataccgtt gctccgatga gtggacgtga
     2401 tatgacgctg gtgcaggtca ccatgaaact tgccaacctt gcagagaaaa actcggcaaa
     2461 attccagcgt gcgcagaaga agaaaaaagc cctggttgat gaaataaccc aactacaggc
     2521 tgacagtgac cagaaagagg atgccatgag cgacctcgcg gatcaggtgg cagcggtaga
     2581 aggccagaag gcagatctgg agcagaaaat taacgtggtt gcttcggaag cggattctct
     2641 ttatcaagaa aatgagcgtt tgcaggcgga gattgatcag ctcaatcgca ctggtgggcg
     2701 cgataccatt gctcctgcgg ggatgactgg tgggcactct cgcgcgctga cggatcgcct
     2761 tgccagtatc aaaaatcgta tgcatatgga cggggaagcg acgctcagta atggtgcatc
     2821 aatgaagcaa ttcattgggg atggcgaagg gtatatccag ttaaccgatc cggatggcag
     2881 cgtatacatg atcaaggcta aatccataca gggtgtggac atggcagaag cgatcggcaa
     2941 gctgtttaaa gcctataaag cgggtaatgt atcggaatat ctggtccaac cagaagaaca
     3001 taaaccggaa aacgtcgaac ctgaaccagc ggaggatacc ggtagctctt cgcctgaacc
     3061 agaagtctct gtaggtgcat atcgatatgc cctgcaaatg cgtccggcgg cccctggcgc
     3121 aatacctgaa ggtaataaag cgattctgcc gcgccctgat gaaggtgacc cgtattatga
     3181 atatgcacgc tacggcattg ctacttacga taccccgctt tctgatcagc aaatgagtga
     3241 gtacgacctg aagttattgc ctcgcgagga ttctttcgac ttcctggcga agacacttac
     3301 taatggtccg tttggcaaat atgcacaaaa agctctggag ctggccacca gctcaccaga
     3361 cgagttccgc gtaatgctga aaactcagtt tcaaaaaact ttccccaata ttgcgtttcc
     3421 tgggggcgct ggcaccgaga aaatggtgca gagcatgatc aatgcattgc aggccgaagt
     3481 cggtgagatt actcagccag aaccggcccc ggcacagcct gatgaaacgg ttagcgaagc
     3541 agatgcagag gctaataaag ccattgaata tctcaataac gtgatggata tgcaaagcac
     3601 tgacatggcg gagatccgta acgcccgggg taatgtccgg gaagcgattg cagcccttca
     3661 ggctgccggg cgttttgagg aaaacgaaga gctggtaaat ggcgctgctc gccacctggc
     3721 tgatctgctg gtagcaatcc agaaagcggg ggtagcggca tgacactatc agctattgag
     3781 ttaatggatc tcagcgataa gttggatgct ctgatgtcca aagcggctac cgcgagtggt
     3841 atggagttgc tggatatcag cgatgaaatt gaccagatca tgcaacagat ggggtacggc
     3901 gcgtctggcg gcggtagtgg cgaggagaaa caaccttcgg aacatgatgg tgtgccaaaa
     3961 ctggttgctg atttcctggc tgataaattc gtcgatcaga gcaccgatgc atttatcggt
     4021 accttgcagg atttgagtca atatgttggc acatacatcg acctggacca ggttaaacag
     4081 cacacggcgg catggatagc cgccaacatt gaagaggcag cataaggcgt aacagggatg
     4141 agcttaagcg atcaggtggt aatggccacc agcatagaaa cgctgatcga gctgctaaag
     4201 aacctgcccg attatgggcg ggtttcgtat gtggtgacag cgaagggaga cgaggtaaaa
     4261 acggcgtttg atatcgtcga tgcctcagct cttttggtat ccaatactct ggatgggaaa
     4321 attaatccag actatcccca ggaacttcag ccgcgcgacc ggacccgcgc atccagcctt
     4381 cttcaggtca accagatatc caaggatttg cgtcctgccc agcttaccga ctccggttta
     4441 tccagccatg gcgcgccgat aattggtgag gacaatgccg ttgagtcagg taatgggcgg
     4501 accatgggga tcattaaagc ctatcaggac ggtaatgcgg atcggtatcg tgagtacctg
     4561 attgaacatg cgaccgaatt cggcatacga cctgaaaagg ttgaatcaat gacggctccg
     4621 gtactggtgc gccgccggtt aactaaggtt gatcgcgttc agtttgccaa ggactcaaat
     4681 atttctgatc ttcaggaaat ggcagccagt gaaaaggctt ttgttgatgc cgacaacata
     4741 actccggcga tgatggcgct gtttaacccg tcagaaagcg gagatctgct tagccgcagt
     4801 aatgacgctt ttatccgcgg attcatgacg caagttggtg ccacacaggc tgctggcctt
     4861 gtaacggaag atgggcgacc aacacggcaa cttgtagacc gtatacaaaa cgcgatcttt
     4921 gccaaggcat ataaggatgc gcgcctggta aggatggttg cagaagaacc tgatccggat
     4981 atgcgtaatg ttctgacggc gcttaatgcg gcggccaatg attttgtcca gatgcaggct
     5041 ttatcaggtg aagcgcacaa gcaggctgtg acaaccattg ttgatggtat tgaaacagcg
     5101 gatagcctcg ataaaaaggc gctggcggca ttgaaagatg cggtagacct ggtaaggcaa
     5161 tcgaaggagt caggccaaca tattaccgat gttattgctc agggggatat gttcagcgaa
     5221 acggccccgg aagtgaaagc actcgcgttg ttcatcgtcg cgaataaccg tagcgcgaag
     5281 cgtatggcca ccgcctttaa gttgatggcg caacgtatca atgatgagtt acagcaccag
     5341 ggccaggcgc tcggggatat gtttggcggc ggcgatgtgt cgttacagga tatccttcgc
     5401 caggtatctc aggaactgga aaacgaaggt atgcaaggga tatctggcgg tcttttcgag
     5461 tcagcttctg gcggtagtta caacggtgtt gctccatata ccagcttgct attacatcgg
     5521 gcatccggca tcaaagacat tattcatctg atcaggctgc tttcccgtac agatccccag
     5581 gatgaacagc ttgttcaagt gcttgcgcat tttgttcgaa tgcctgttgc cgacgtgaaa
     5641 aaatggtgcc gattattcgg tatcagcaat tcgttacttc gcggcttgtt aaatcacgca
     5701 tcctcccttg ggcgcgatgg ctttgacgag atagcgcagg cgataaaaaa cggagatatg
     5761 ccaccagcta ttgactggtt ttccattcgc ccaaccaggg tgaaagcatt ccttagcgcg
     5821 gcgcattcgg catcaccatt ggcagaaatg gttcagaggt tatcgctcat attcacagac
     5881 cataccgcgt tgggtgatct gactctggac gagatgaagg aagcctccat tcagtgggcc
     5941 gatcaacaaa atgaggttaa ctcagacttc ttgccagcat tcaggaaggc cgttagtaaa
     6001 gcggatgatg cccgtggaat tctgaaggca tttaaggcat tgcaaagtcg tgttaataaa
     6061 catgtcggtg atatcgatgg ggtaacggcg gaaggccggg atatccttaa agagcacggc
     6121 ataacgccag agtttattga tgagatcagg actgatatgc agcgtgaggt cgtatcgtcc
     6181 ctgcaaatcg tagccagagc gttggcggat gctaatccga agagtgcggc cattgttaac
     6241 cgggttattg gtgatattga agcatcggag ggcatggggg cgctgaaact cttcctttcg
     6301 cgagcgttta atcctaacgg caatattctc cctggcatta ttggtgaggc taaaaagtat
     6361 gtcagtgaag aagaacttga gcagcttgac caactactta agcgattctc atataacccg
     6421 cagacacgct ggcaaatgaa tcagcgaagt atgggttcgg tccacgagaa agtgttatct
     6481 gccatgaaca gtgcgatcgc aaactcatcc gtatctgaag aaaaagctct tgagtgggcc
     6541 gactctttta tcacagaaga agtggaagaa gcccgcgctg gacagaatgg tgggatagac
     6601 ctgcgcaagg aacttgctga tatttatcgc ctgaccggcg ggaaaatatc gaccttatca
     6661 aaggtagttc accaccaggg aagggcatat gcaaatctaa atggtgttgt tgctgtcaat
     6721 ttgaacgatg aaaatgcgag cgcactgtgg cacgagctgg gtcatcatct tgagtacagt
     6781 aaccctggtt tgttagagaa agcccggtca ttcctgaagg ccaatgttga aggggataag
     6841 ccatctttcg tcaatatcgg tgggcgtggc aagcctgaat ggtgcttcag atctcgattg
     6901 agtaatattt atatggcgaa ggtatacccg ccagcctcag taagtaacac cgggaaaatt
     6961 cggcagaaat caccgactat ttccaaaacg tcagcaacgg aagtattctc tatggctctt
     7021 cagttgtatc atgataaaga ggccgctgcc gcatcactga tgaatggtga cggattgctg
     7081 gaactgttat taggtgtggc aaaggagcta aataatgcag attaaaatcg cagcgccatt
     7141 aggcggagat gccattatcg aatttgatga taatgaagaa gtttccgggc gtttaagcat
     7201 tatctccggt gacattaccg aggacatgat cgctgaagcc atagctgggg caaatcccaa
     7261 tagctatatg ggattcgtta acacccttga tgctcccgca agtgatgttc tccgaacgct
     7321 gcatctttac gctggctggt ttgttgattg gccagcagta gagggtggcg atgaggacga
     7381 cgacgatgat tttggtgatc atgtagacca gatcgtatat tgaagaaatc ccgccgattg
     7441 gcgggatttc ttgtttatgc caggcgggct atgcgtattt tacaatcatc taggtggatg
     7501 cgtagtgctt ctaaacatgg agtaaagtta gctgagaatt tgtcggcctc acaggccaat
     7561 gcataaaatt tttcagctaa ttcaagagat tcatcccata tttttcgact tacaaagtct
     7621 ggtagtgctg tttttctaag ctcattagac attgctcccc cagagcgtgg ggcaaaaccc
     7681 ataggtaaca tgtcataggc tggagcaata tgatatgggc gtccgtggct actaataaac
     7741 gataggttgc cattatgcat atcggtattg cctattagca caccaaaaga ccatagcctt
     7801 gttgctataa taatcgcatc aggatggacg cacttatttt ttaccaactc tttaaccagc
     7861 aagggccatg ttgctcttgc attaccaaca aattctgcat caagtgcacg aagtgagaat
     7921 acacctaccc gaccgagttg accaacacga tcaaatcggg gaatttccaa gaatctctga
     7981 ccgttataat caaaaacttc tgtttctacc cccagaactt ttaatgcgag atgttcagca
     8041 aacaagagat ctctccagcg ctcacttata ggggtgtcgt taggcgcaga gaatttcact
     8101 aatacatgtc cacgctctgt gaaagtacaa aatttgggtt gttcacctcc agcagatgaa
     8161 cctggcacat caccttcacc agcggctaaa gctaatacag gatattcaat ttcacgatta
     8221 acaggaacag gaggctgcat ctctaaaaaa tgcgtgcgcg cttgctctcc aattaacaaa
     8281 ttaccaacaa catcatgccc atgcttaagt aaggctttga tcacatcagt atctgaccat
     8341 tgctctggat ttgagggaag gttaagttca agagcatatg ctgatgcata tgctcttcca
     8401 aggaagcctt gtggacgcat atcaaataac caccaaggta acccatcgct atgcctgtta
     8461 tttttcccat cctcaaccat aacatagcct tctggcctca ccggaatgag agtaccaaga
     8521 acgcaaatac acccctcttc agttatacga taaatgggga ctgattcgaa tcctctaaaa
     8581 gagtctcgta aagcatattg aatagatctt ccatttccga tcctaacgat ttcatcacca
     8641 agttctctta atgctctaga tatcgttggt tggctaacac tgagtttttc aaccaattgt
     8701 cttggtgtca taggcccttg attgagcaag ctacggattg ttgttgcatg tgtagccata
     8761 gttttaatta ctg
//
LOCUS       DAZGJW010000176         8740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZGJW010000176 DAZGJW010000000
VERSION     DAZGJW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.176"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..202)
                     /locus_tag="ACHKIK_004361"
     CDS             complement(<1..202)
                     /locus_tag="ACHKIK_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085412.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALP"
     gene            complement(445..651)
                     /locus_tag="ACHKIK_004362"
     CDS             complement(445..651)
                     /locus_tag="ACHKIK_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO9085413.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            964..1053
                     /gene="kdpF"
                     /locus_tag="ACHKIK_004363"
     CDS             964..1053
                     /gene="kdpF"
                     /locus_tag="ACHKIK_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO9085414.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            1053..2726
                     /gene="kdpA"
                     /locus_tag="ACHKIK_004364"
     CDS             1053..2726
                     /gene="kdpA"
                     /locus_tag="ACHKIK_004364"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO9085415.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLDQHYLPLNPQQLPGLSWDLA
                     LNAAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGSVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2749..4797
                     /gene="kdpB"
                     /locus_tag="ACHKIK_004365"
     CDS             2749..4797
                     /gene="kdpB"
                     /locus_tag="ACHKIK_004365"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO9085416.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSARGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4806..5378
                     /gene="kdpC"
                     /locus_tag="ACHKIK_004366"
     CDS             4806..5378
                     /gene="kdpC"
                     /locus_tag="ACHKIK_004366"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO9085417.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5371..8055
                     /gene="kdpD"
                     /locus_tag="ACHKIK_004367"
     CDS             5371..8055
                     /gene="kdpD"
                     /locus_tag="ACHKIK_004367"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO9085418.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLTDPAEEKAVVRYARE
                     HNLGKIILGRPASCRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            8052..8729
                     /gene="kdpE"
                     /locus_tag="ACHKIK_004368"
     CDS             8052..8729
                     /gene="kdpE"
                     /locus_tag="ACHKIK_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO9085419.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
BASE COUNT         1827 a         2272 c         2537 g         2104 t
ORIGIN      
        1 cgggcagggc gatgtcggtt tcaccgggaa ggacctttgc accgagcagc ggattgaccg
       61 gatggccgga cgtcaccccg ccggggcaca ccgaacaggc cacgccggtg ggggcaccga
      121 tgcgcacccc ggctgaaccc tggacaatgc taccgccata ctgcgtcatg tcgccctgac
      181 gcgctgccgg ttttccgctc atctgctcat ccttgtttta tcgggttatt tgtctgcttc
      241 caggcaaaac cctacagatt tataagaaag ccttaagtga gatatctgtc agataagaac
      301 aattaggaaa atatttatat atatttcatg aatatatgtt tcgattggta taacgatgtc
      361 taaatattcc tctgaacatc ttcctgatac aaaaaaccgc cagcgtttcc gctggcggtt
      421 ttcaatgtat tcaaaactca gcaatcaata aaaatcacca gttgcctttt cggcctgatc
      481 catccacacc ggtttatcgc tggttttcga ccagacgcga tgcaggtagc tgtaaaagcg
      541 ggcgcggtct ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata
      601 agtacggcgt aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc
      661 ccccttttgt atctacccgg tgaatggtac cggaaaaatg aatttgttta tctgatgaaa
      721 atagtaccgc cttttgtgta attttactac tcatccgacc acttattttt gcttattgat
      781 ggtttattta cattcatcct gtaattaagt tacacaaaag ttaaattaat actaaacatt
      841 agttaaatca tggcttttgc catttttata ctttttttac accccgcccg cagatttttg
      901 cgaaatcttt gcagccagaa ttctaccctt ccggtatcac ttttaggcca ctggaggtgc
      961 actgtgagtg caggcgtgat aaccggcgta ttgctggtgt ttttattact gggttatctg
     1021 gtttatgccc tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgatcg
     1081 ccacgttttt actggtgtta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc
     1141 tgattaatga cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac
     1201 ctggcgtctc tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga
     1261 acatgctggg gctggcggtg ctgtttttta tgttgctcga tcagcactat ctgccgctta
     1321 atccacagca gttgccgggg ctgtcgtggg atctggcgct gaatgccgcc gtcagctttg
     1381 tcacgaatac caactggcaa tcttacagcg gtgaaaccac gttgagctat ttcagccaga
     1441 tggctggctt aacggtgcaa aactttcttt ctgccgccag cgggattgcg gtgatttttg
     1501 ccctcatccg tgcgtttacc cgccagagca tgagcacact cgggaatgcc tgggtcgatc
     1561 tgctacgcat cacgttatgg gtgctcgtcc ctgtggcgtt gttgatcgca ctgtttttta
     1621 ttcaacaagg tgcgctgcaa aactttcagc cttatcaggc tgtgaatacc gttgaaggag
     1681 cgcaacagct gttacccatg gggcctgtag cttctcagga agcgatcaag atgctcggta
     1741 ctaacggcgg tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac
     1801 tgaccaactt cgtgcagatg ctggcgatct tcttgatccc aacggcgctg tgctttgcct
     1861 ttggtgaagt gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga
     1921 tttttgtcat ctgcgtaggc gtggtgatgt gggcagaagt tcagggtaat cctcatctgc
     1981 tggcgctcgg cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtac
     2041 tggtcagtag cctgtttgcg gtcgtgacta cggcggcttc ctgtggcgcg gtgattgcga
     2101 tgcatgattc gtttaccgct ctcggtggca tggtgccgat gtggctgatg caaattggtg
     2161 aagtggtgtt cggcagtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg
     2221 cggtgtttat tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatcg
     2281 acgtacgcga gatgaaactg actgcactgg cgattctggt taccccgacg ctggtgctga
     2341 tgggcgcggc gctggcaatg atgaccgacg ccggacgtag cgccatgctc aaccctggcc
     2401 cgcatggttt tagcgaagtg ctgtacgccg tgtcatccgc cgctaacaac aacggcagcg
     2461 cctttgccgg attaagcgcc aactccccgt tctggaactg tttactggcg ttctgcatgt
     2521 ttgtcggtcg cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta
     2581 aaaagagcca acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc
     2641 tgttaatcgg caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg
     2701 gtccggtggc ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa
     2761 caactggcgc tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa
     2821 ttaaacccgc aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg
     2881 ctgaccacct gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt
     2941 agcgcggcaa ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg
     3001 ctggcagaag gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc
     3061 tttgcccgca agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat
     3121 caactgcgta aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt
     3181 gaagtcattg aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg
     3241 gtgatccgtg aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct
     3301 gactggctgg tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc
     3361 gcgatggtgg aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg
     3421 ctgattgctc tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg
     3481 aggggcggta atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca
     3541 accactattg gcggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc
     3601 gcgaatgtga ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg
     3661 ctactggata aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatccct
     3721 gcgcagggcg tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat
     3781 gaaacgccgg aaggccgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag
     3841 cgcgatgtgc agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc
     3901 gggatcaaca tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat
     3961 gttgaggcta acggcggtca cttccctacc gatgttgatc aaaaagtcga tcaggttgcg
     4021 cgtcaggggg ccacgccgct ggtggtggtg gaaggttccc gtgtgctggg cgttattgcg
     4081 ctgaaagata tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaaatgggc
     4141 attaaaacgg tgatgattac cggcgataac cgtctgactg ccgccgccat tgctgcggaa
     4201 gcgggcgtcg atgattttct cgccgaagcg acaccggagg ccaagctggc attgattcgt
     4261 cagtatcagg cggaaggccg tttggtggcg atgaccggcg acggcaccaa cgatgctcca
     4321 gcgctggcgc aggcagatgt cgcggtggcg atgaactccg gtactcaggc ggcgaaagaa
     4381 gcgggcaata tggtcgatct cgactctaac ccgaccaagt tgatcgaggt ggtacatatt
     4441 ggcaaacaga tgctgatgac ccgtggctcg ctgaccacct tcagcattgc caacgatgtg
     4501 gcgaaatact tcgccattat tccggcggcg ttcgcggcaa cgtatccgca gttaaatgcg
     4561 ctgaacatca tgcgccttca ttcgcccgac tccgccattc tcagtgcggt cattttcaac
     4621 gccttgatta tcgtcttttt gattcctctg gcgttaaaag gcgtgagtta taaaccgctt
     4681 accgcttctg ccatgttgcg ccgtaactta tggatttacg gtctgggtgg gctgctggtg
     4741 ccgtttattg gtatcaaagt cattgattta ctgctgaccg tttgcggtct ggtgtgaggt
     4801 ttaccatgcg tggcttacgt ccggcattat cgacatttat ctttctgtta ttgattactg
     4861 gcggcgttta cccgctgctg accaccgtgc tggggcaatg gtggtttccc tggcaggcca
     4921 acggttcgtt gattcgtgaa ggtgatacgg tgcggggttc ggcattaatc gggcagaatt
     4981 ttaccggcaa cggctatttt cagggtcgcc cgtcggcaac ggcagaaatg ccctataatc
     5041 cacaggcttc tggcgggagc aatctggcgg tcagtaaccc tgagctggat aaacaaatag
     5101 ccgcacgcgt tgctgcatta cgggccgcta acccggatgc cagtacgaac gttccggttg
     5161 aactggtgac ggcatcggca agcgggctgg acaataatat caccccgcaa gcggcggtct
     5221 ggcaaatccc acgcgtggcg aaagcgcgta atctcagcgt tgaacagctc acgcaactga
     5281 tcgcaaaata cagccaacaa ccgctggtga aatatatcgg ccagccggtc gtcaacattg
     5341 ttgaactcaa tctggcgctg gataaacttg atgaataacg aacccttacg tcccgacccc
     5401 gatcgtctgc tggaacaaac tgccgcgccg catcggggga agctgaaagt tttcttcggt
     5461 gcctgtgcag gcgtcgggaa gacctgggcg atgctggcag aagcccagcg actgcgggcg
     5521 caagggctgg atattgtggt tggcgtggta gaaacccatg ggcggaaaga taccgccgcc
     5581 atgcttgaag ggctggctgt tctgccgccc aaacgccagg cgtaccgtgg gcggcatatc
     5641 agcgagtttg atctcgatgc cgccctcgcc cgccgtccgg cgctgatctt aatggacgaa
     5701 ctggcgcaca gtaatgcgcc aggttctcgt catcctaaac gctggcagga tatcgaagaa
     5761 ctgctggaag ctggcattga tgttttcaca accgtcaacg ttcagcatct ggaaagtctg
     5821 aatgatgtgg tcagcggcgt caccggaatt caggtacggg aaaccgtgcc cgatcctttt
     5881 ttcgatgccg ccgacgacgt agtgctggtg gacttgcccc cggacgatct gcgccagcgg
     5941 ctgaaagaag gcaaagtcta tattgccggg caggcggagc gcgccattga acattttttc
     6001 cgcaaaggca atctgatcgc cctgcgtgaa ctggcactgc gccgtactgc cgatcgcgta
     6061 gatgagcaaa tgcgcgcctg gcgggggcat cctggtgaag agaaagtgtg gcacacgcgc
     6121 gacgcgatcc ttttatgcat cgggcataac accggcagtg agaaactggt ccgcgcggcg
     6181 gcgcggttgg cctcgcgcct gggtagcgtc tggcacgcgg tgtatgttga aacccctgcc
     6241 ctgcatcgct taccggaaaa aaaacgtcgg gcaattctca gcgccttacg tctggcgcag
     6301 gagctgggcg cggagacggc tacacttact gatccagcgg aagaaaaagc agtagtgcgt
     6361 tatgcccgtg aacataatct cggcaagatt attctcggtc gcccggcctc gtgccgctgg
     6421 tggcgtcggg aaacgtttgc tgaccgactg gcgcgcatcg cccccgatct cgatcaggtg
     6481 ctggtcgcgc ttgatgaacc acccgcccgc acgattaaca acgcgccgga tagccgctct
     6541 tttaaagaca agtggcgggt acagattcag ggatgcgtgg ttgccgccgc gttatgcgcc
     6601 gttatcactt taattgccat gcagtggctg atggcgtttg atgccgccaa cctggtgatg
     6661 ctgtatctgc ttggcgtggt ggtggtggcg ctattttatg gacgctggcc ttcggtggtt
     6721 gctaccgtca ttaatgtggt gagtttcgat ctctttttta tcgccccacg cggcacgctc
     6781 gctgtctctg atgtgcaata tctgctgacc ttcgcggtga tgttaaccgt cgggctggtg
     6841 atcgggaacc ttactgcggg tgtgcgttat caggcgcggg tagcccgtta ccgcgagcaa
     6901 cgcacacggc acttatatga aatgtcgaaa gctctggcgg tgggccgcag tccgcaggac
     6961 atcgccgcca ccagcgaaca atttattgcc tccacgtttc atgcccgcag tcaggtgttg
     7021 ttgcccgatg acaacggtaa attgcagccg ttaacacatc cgcaaggaat gacgccatgg
     7081 gacgatgcca tcgcgcagtg gagttttgat aaaggcctgc ctgcgggtgc gggcaccgac
     7141 acgttacccg gcgtgccgta ccagattttg ccgctaaaaa gcggcgagaa aacctacgga
     7201 ctggtggtgg tggaaccggg gaatctgcgt cagttgatga tcccggagca gcagcgcctg
     7261 ctagagacgt ttacgctgtt agtcgccaat gcgtttgagc ggctgacgct gaccgccagt
     7321 gaagaacagg cgcggatggc aagcgaacgt gaacagatcc gcaacgccct gctggcggcg
     7381 ctttcgcatg atttacgcac gccgcttacg gtgctgtttg gtcaggcaga aatcttaacg
     7441 ctcgatctgg caagcgaagg atcgccccac gcccgccagg ccagcgagat tcgtcagcat
     7501 gtgctgaaca ctacccgact ggtgaataat ctactggata tggcgcgaat tcagtccggc
     7561 ggctttaatt tgaagaaaga gtggttaacg ctggaagaag tagtcggcag cgctctgcaa
     7621 atgctggaac cgggtttatc gtcgcccatc aatctttctc tgccagaacc gctgacctta
     7681 atccacgttg acgggccact ctttgaacgg gtgctgatta atctgctgga gaacgcggtg
     7741 aaatatgcgg gtgcgcaggc cgaaattggt atcgatgccc acgttgaggg cgaaaatcta
     7801 caactggatg tctgggataa cggccccggt cttccgccag gccaggagca gacgatattt
     7861 gataagtttg ctcgcgggaa taaagagtcg gcagtaccgg gggtagggct tggactggca
     7921 atttgtcggg cgatagtgga tgtacacggg ggcactatta ccgcgttcaa ccgaccggaa
     7981 ggtggtgcct gttttcgtgt tacacttccc cagcaaaccg ctcctgaact tgaagacttt
     8041 catgaggata tgtgacaaac gttctgattg ttgaagatga acaggctatt cgtcgctttc
     8101 tgcgcacggc gctggagggc gacgggatgc gcgtctatga ggccgaaacg ctgcaacgcg
     8161 gcttgctgga agcggctacc cgtaagccag atttgattat tctcgatctc ggcctgcccg
     8221 atggtgatgg gattgagttt atccgcgacc tgcgccagtg gagcgcggtg ccggtgattg
     8281 tgctttccgc acgcagcgaa gagagcgaca aaatcgccgc gctggatgcc ggagcggatg
     8341 attatctgag taagccgttt ggcattggcg aattgcaggc ccgtctgcgc gtcgctttac
     8401 gccgccactc cgccaccgcc gcgcccgatc cgctggtgaa attttccgat gttaccgtcg
     8461 atttagccgc ccgcgtgatt caccggggtg aggaagaggt gcatctcacg ccgattgagt
     8521 tccgcctgct ggcggtgctg ctaaataatg ccgggaaagt gctcacccag cgccagttac
     8581 tcaatcaggt gtgggggcca aacgcggtcg aacacagtca ctatttgcgt atttatatgg
     8641 gacatctgcg acaaaaactg gaacaggatc ccgctcgccc ccgccatttc attactgaaa
     8701 ccggtattgg gtatcggttt atgccatgaa tgtttattca
//
LOCUS       DAZGJW010000177         8693 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZGJW010000177 DAZGJW010000000
VERSION     DAZGJW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.177"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(353..616)
                     /gene="rpsT"
                     /locus_tag="ACHKIK_004369"
     CDS             complement(353..616)
                     /gene="rpsT"
                     /locus_tag="ACHKIK_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO9085420.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            719..937
                     /locus_tag="ACHKIK_004370"
     CDS             719..937
                     /locus_tag="ACHKIK_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO9085421.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            945..1886
                     /gene="ribF"
                     /locus_tag="ACHKIK_004371"
     CDS             945..1886
                     /gene="ribF"
                     /locus_tag="ACHKIK_004371"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO9085422.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLGVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1929..4745
                     /gene="ileS"
                     /locus_tag="ACHKIK_004372"
     CDS             1929..4745
                     /gene="ileS"
                     /locus_tag="ACHKIK_004372"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO9085423.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4745..5239
                     /gene="lspA"
                     /locus_tag="ACHKIK_004373"
     CDS             4745..5239
                     /gene="lspA"
                     /locus_tag="ACHKIK_004373"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO9085424.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5327..5776
                     /gene="fkpB"
                     /locus_tag="ACHKIK_004374"
     CDS             5327..5776
                     /gene="fkpB"
                     /locus_tag="ACHKIK_004374"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO9085425.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5778..6728
                     /gene="ispH"
                     /locus_tag="ACHKIK_004375"
                     /gene_synonym="lytB"
     CDS             5778..6728
                     /gene="ispH"
                     /locus_tag="ACHKIK_004375"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO9085426.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6794..7708
                     /gene="rihC"
                     /locus_tag="ACHKIK_004376"
     CDS             6794..7708
                     /gene="rihC"
                     /locus_tag="ACHKIK_004376"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO9085427.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            7875..>8693
                     /gene="dapB"
                     /locus_tag="ACHKIK_004377"
     CDS             7875..>8693
                     /gene="dapB"
                     /locus_tag="ACHKIK_004377"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO9085428.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         1979 a         2155 c         2477 g         2082 t
ORIGIN      
        1 ttatttacaa gatctaataa ggagatattt atgaatggtt aaagtgtata ggaatttaat
       61 tcaaaaatag agtggtctta agtttaatac ctcaatgtgt atcacaatat ccacattctt
      121 tgtaggggag tctggagatt gagtagatat tcttgttcag aatgtatcag ccgatggttc
      181 tacgattctt aagccacgaa gagttcagaa agtgtaacgg catgtctgca aaagccagca
      241 tcttttgact atctggcaac cggtagtgtg ttctctcacg catcataaaa gcagcaggca
      301 taaaaaaacc cgcttgcgcg ggctttttca caaagcttca gcaaattggc gattaagcca
      361 gtttgttgat ctgtgcagtc aggttagcct tatgacgtgc agctttgttt ttgtggatca
      421 gacctttagc agcctgacgg tccacgatcg gttgcatttc gttaaatgct ttctgtgcag
      481 cagctttgtc gccagcttcg atagctgcgt atactttctt gatgaaagta cgcatcatag
      541 agcgacggct tgcgttgtgc ttacgagcct tttcagactg aatggcgcgc ttcttagctg
      601 atttgatatt agccaaggtc caactcccaa atgtgttcta tatggacaat tcaaaggccg
      661 aggaatatgc ccttttagcc ttcttttgtc aatggatttg tgcaaataag cgccgttaat
      721 gtgccggcac tcgttacgta gtgatggcgc aggattctac cagcttgcag ggtgtgaata
      781 cagcttttcc gcgataaaaa ttgcagcagg cggtcacttt cttcccgtga tttgcgccat
      841 ggcaatgaaa agccacttct ttctgatttc ggtactcaat cgccggttaa ccttgaccgc
      901 tgtacaaggt atactcggac gattttcact gttttgagcc agacatgaag ctgatacgcg
      961 gcatacataa tctcagccag gccccgcaag aagggtgtgt gctgactatt ggtaatttcg
     1021 acggcgtgca tcgcggtcat cgcgcgctgt tacagggctt gcaggaagaa gggcgcaagc
     1081 gcaacttacc ggtgatggtg atgctttttg aacctcaacc actggaactg tttgctaccg
     1141 ataaagcccc ggcacggctg acccggctgc gggaaaaact gcgttacctt gcagagtgtg
     1201 gcgttgatta cgtgctgtgc gtgcgtttcg acaggcgttt cgcggcgtta accgcgcaaa
     1261 atttcatcag cgatcttctg gtgaagcatt tgcgcgtaaa atttcttgcc gtaggtgatg
     1321 atttccgctt tggcgctggt cgtgaaggcg atttcttgtt attacagaaa gctggcatgg
     1381 aatacggctt cgatatcacc agtacgcaaa ctttttgcga aggtggcgtg cgtatcagca
     1441 gcaccgctgt gcgtcaggcg ctggcggatg acaatctggc tctggcagaa agtttactgg
     1501 ggcacccgtt tgccatctcc gggcgtgtag tccacggtga tgaattaggg cgcactatag
     1561 gtttcccgac ggcgaatgta ccgctacgcc gtcaggtttc cccggtgaaa ggggtttatg
     1621 cggtagaagt gctgggcctc ggtgaaaagc cgttacccgg cgtggcaaac attggaacac
     1681 gcccaacggt tgccggtatt cgccagcagc tggaagtgca tttgttaggt gttgcaatgg
     1741 acctttacgg tcgccatata caagtagtgc tgcgtaaaaa aatacgcaat gagcagcgat
     1801 ttgcgtcgct ggacgaactg aaagcgcaga ttgcgcgtga tgaattaacc gcccgcgaat
     1861 tttttgggct aacaaaaccg gcttaagcct gttatgtaat caaaccgaaa cacggaaccg
     1921 agaatctgat gagtgactat aaatcaaccc tgaatttgcc ggaaacaggg ttcccgatgc
     1981 gtggcgatct cgccaagcgc gaaccgggaa tgctggcgcg ttggactgat gatgatctgt
     2041 acggcatcat ccgtgcggct aaaaaaggca aaaaaacctt cattctgcat gatggccctc
     2101 cttatgcgaa tggcagcatt catattggtc actcggttaa caagattctg aaagacatta
     2161 tcgtgaagtc caaagggctt tccggttatg actcgccgta tgtgcctggc tgggactgcc
     2221 acggtctgcc gatcgagctg aaagtagagc aagaatacgg taagccgggt gagaaattca
     2281 ccgccgccga gttccgcgcc aagtgccgcg aatacgcggc gacccaggtt gacggtcaac
     2341 gcaaagactt tatccgtctg ggcgtgctgg gcgactggtc gcacccgtac ctgaccatgg
     2401 acttcaaaac tgaagccaac atcatccgcg cgctgggcaa aatcatcggc aacggtcacc
     2461 tgcacaaagg cgcgaagcca gttcactggt gcgttgactg ccgttctgcg ctggcggaag
     2521 cggaagttga gtattacgac aaaacttctc cgtccatcga cgttgctttt caggcggtcg
     2581 atcaggatgc actgaaagca aaatttgccg taagcaacgt taacggccca atctcgctgg
     2641 tgatctggac cactacgccg tggactctgc ctgcgaaccg cgcaatctct attgcacctg
     2701 atttcgacta tgcgctggtg cagatcgacg gtcaggccgt gattctggcg aaagatctgg
     2761 ttgaaagcgt aatgcagcgt atcggcgtga ccgattacac cattctcggc acggtaaaag
     2821 gtgcggagct tgagttgctg cgctttaccc atccgtttat gggcttcgac gttccggcaa
     2881 tcctcggcga tcacgttacc ctggatgccg gtaccggtgc cgttcacacc gcgcctggcc
     2941 acggcccgga cgactatgtg atcggtcaga aatacggcct ggaaaccgct aacccggttg
     3001 gcccggacgg cacttatctg ccgggcactt atccgacgct ggatggcgtg aacgtcttca
     3061 aagcgaacga catcgtcgtt gcgctgctgc aggaaaaagg cgcgctgctg cacgttgaga
     3121 aaatgcagca cagctatccg tgctgctggc gtcacaaaac gccgatcatc ttccgcgcga
     3181 cgccgcagtg gttcgtcagc atggatcaga aaggtctgcg tgcgcagtca ctgaaagaga
     3241 tcaaaggcgt gcagtggatc ccggactggg gccaggcgcg tatcgagtcg atggttgcta
     3301 accgtcctga ctggtgtatc tcccgtcagc gcacctgggg cgtaccgatg tcactgttcg
     3361 tgcacaaaga cacggaagag ctgcatccgc gtactctcga actaatggaa gaagtggcaa
     3421 aacgcgttga agttgacggc atccaggcgt ggtgggatct tgatgcgaaa gagatcctcg
     3481 gcgatgaagc tgatcagtac gtgaaagtgc cggacacatt ggatgtatgg tttgactccg
     3541 gatctaccca ctcttctgtt gttgacgtgc gtccggaatt tgccggtcac gcagcggaca
     3601 tgtatctgga aggttctgac cagcaccgtg gttggttcat gtcttcccta atgatctcca
     3661 ccgcgatgaa aggcaaagcg ccgtatcgtc aggtactgac ccacggcttt accgtggatg
     3721 gtcagggtcg caagatgtct aaatccatcg gcaataccgt ttcgccgcag gatgtgatga
     3781 acaaactggg cgcggatatt ctgcgtctgt gggtggcatc aaccgactac accggtgaaa
     3841 tggccgtttc tgacgagatc ctgaaacgtg ctgccgatag ctatcgtcgt atccgtaaca
     3901 ccgcgcgctt cctgctggca aacctgaacg gttttgatcc agcaaaagat atggtgaaac
     3961 cggaagagat ggtggtactg gatcgctggg ccgtaggttg tgcgaaagcg gcacaggaag
     4021 acatcctcaa ggcgtacgaa gcatacgatt tccacgaagt ggtacagcgt ctgatgcgct
     4081 tctgctccgt tgagatgggt tccttctacc tcgacatcat caaagaccgt cagtacaccg
     4141 ccaaagcgga cagcgtggcg cgtcgtagct gccagactgc gctgtatcac atcgctgaag
     4201 cgctggtgcg ctggatggca ccaatcctct ccttcaccgc tgatgaagtg tggggctatc
     4261 tgccgggcga acgtgaaaaa tacgtcttta ccggtgagtg gtacgaaggc ctgtttggtc
     4321 tggcagacag tgaagcgatg aacgatgcgt tctgggacga gctgttgaaa gtgcgtggcg
     4381 aagtgaacaa agtcattgag caagcgcgtg ccgataagaa agtgggtggc tcgctggaag
     4441 cggcagtaac cttgtatgca gaaccggaac tggcggcgaa actgaccgcg ctgggcgatg
     4501 aattacgatt tgtcctgttg acctccggcg ctaccgttgc agactataat gacgcacctg
     4561 ctgatgctca gcagagcgaa gtactcaaag ggctgaaagt cgcgttgagt aaagccgaag
     4621 gtgagaagtg cccacgctgc tggcactaca cccaggatgt cggcaaggtg gcggaacacg
     4681 cagaaatctg cggccgctgt gtcagcaacg tcgccggtga cggtgaaaaa cgtaagtttg
     4741 cctgatgagt caatcgatct gttcaacagg gctacgctgg ctgtggctgg tggtagtcgt
     4801 gctgattatc gatctgggca gcaaatacct gatcctccag aactttgctc tgggggatac
     4861 ggtcccgctg ttcccgtcgc ttaatctgca ttatgcgcgt aactatggcg cggcgtttag
     4921 tttccttgcc gatagcggcg gctggcagcg ttggttcttt gccggtattg cgattggtat
     4981 tagcgtgatc ctggcagtga tgatgtatcg ctcgaaggcc acgcagaagc taaacaatat
     5041 cgcttatgcg ctgattattg gcggcgcgct gggcaacctg ttcgaccgcc tgtggcacgg
     5101 cttcgttgtc gatatgatcg acttctacgt cggcgactgg cacttcgcca ccttcaacct
     5161 tgccgatact gccatttgtg tcggtgcggc actgattgtg ctggaaggtt ttttgccttc
     5221 taaagcgaaa aaacaataat aaaccctgcc ggataaggcc tacagattgc tgcgaaatcg
     5281 taggccgcat ccggcaaaaa tcctcaaaat ataagagcaa acctgcatgt ctgaatctgt
     5341 acagagcaat agcgccgtcc tggtgcactt cacgctgaaa ctcgatgatg gcaccaccgc
     5401 tgagtctacc cgcaacaacg gtaaaccggc gctgttccgc ctgggcgatg cttctctttc
     5461 tgaagggctg gagcaacacc tgttggggct gaaagtgggc gataaaacca ccttctcgct
     5521 ggagcccgat gcggcgtttg gcgtgccgtc accggacctg attcagtact tctcccgccg
     5581 tgaatttatg gatgcaggcg agccagaaat tggcgcaatt atgcttttta ccgcaatgga
     5641 tggcagtgag atgcctggcg tgatccgcga aattaacggc gactccatta ccgttgattt
     5701 caaccatccg ctggccgggc agaccgttca ttttgatatt gaagtgctgg aaatcgatcc
     5761 ggcactggag gcgtaacatg cagatcctgt tggccaaccc gcgtggtttt tgtgccgggg
     5821 tagaccgcgc tatcagcatt gttgaaaacg cgctggccat ttacggcgca ccgatatatg
     5881 tccgtcacga agtggtacat aaccgctatg tggtcgatag cttgcgtgag cgtggggcta
     5941 tctttattga gcagattagc gaagtaccgg acggcgcgat cctgattttc tccgcacacg
     6001 gtgtttctca ggcggtacgt aacgaagcaa aaagtcgcga tttgacggta tttgacgcga
     6061 cctgtccgct ggtgaccaaa gtgcatatgg aagtcgcccg cgccagtcgc cgtggcgaag
     6121 aatctattct catcggtcac gccgggcacc cggaagtgga agggacaatg ggccagtaca
     6181 gtaacccgga agggggaatg tatctggtcg aatcgccgga cgatgtgtgg aaactgacgg
     6241 tcaaaaacga agagaagctc tcctttatga cccagaccac gctgtcggtg gatgacacgt
     6301 ctgatgtgat cgacgcgctg cgtaaacgct tcccgaaaat tgtcggtccg cgcaaagatg
     6361 acatctgcta cgccacgact aaccgtcagg aagcggtacg cgccctggca gaacaggcgg
     6421 aagttgtgtt ggtggtcggt tcgaaaaact cctccaactc caaccgtctg gcggagctgg
     6481 cccagcgtat gggcaaacgc gcgtttttga ttgacgatgc gaaagatatc caggaagagt
     6541 gggtgaaaga ggttaaatgc gtcggcgtga ctgcgggcgc atcggctccg gatattctgg
     6601 tgcagaatgt ggtggcacgt ttgcagcagc tgggcggtgg tgaagccatt ccgctggaag
     6661 gccgtgaaga aaacattgtt ttcgaagtgc cgaaagagct gcgtgtcgat attcgtgaag
     6721 tcgattaagt cattagcagc ctaagttatg cgaaaatgcc ggtcttgtta ccggcatttt
     6781 ttatggagaa aacatgcgtt tacctatctt cctcgatact gaccccggca ttgacgatgc
     6841 cgtcgccatt gccgccgcga tttttgcacc cgaactcgac ctgcaactga tgaccaccgt
     6901 cgcgggtaat gtctcggttg agaaaactac ccgcaatgcc ctgcaactgc tgcatttctg
     6961 gaatgcggag attccgctcg cccaaggggc cgctgtgccg ctggtacgcg caccgcgtga
     7021 tgcggcatct gtgcacggcg aatcgggaat ggctggctac gactttgttg agcacaaccg
     7081 aaagccgctc gggataccgg cgtttctggc gattcgggat gccctgatgc gtgcaccaga
     7141 gcctgttacc ctggtggcca tcggcccgtt aaccaatatt gcgctgttac tttcacaatg
     7201 cccggaatgc aagccgtata ttcgccgtct ggtgatcatg ggtggttctg ccggacgcgg
     7261 caactgtacg ccaaacgccg agtttaatat tgctgccgat ccagaagctg ctgcctgtgt
     7321 cttccgcagt ggtattgaaa tcgtcatgtg cggtttggat gtcaccaatc aggcaatatt
     7381 aactcctgac tatctctcta cactgccgca gttaaaccgt accgggaaaa tgcttcacgc
     7441 cctgtttagc cactaccgta gcggcagtat gcaaagcggc ttgcgaatgc acgatctctg
     7501 cgccatcgcc tggctggtgc gcccggacct gttcactctc aaaccctgtt ttgtggcagt
     7561 ggaaactcag ggcgaattta cctcaggcac gacggtggtt gatatcgacg gttgcctggg
     7621 caagccagcc aatgtacagg tggcattgga tctggatgtg aaaggcttcc agcagtgggt
     7681 ggctgaggtg ctggctctgg cgtcgtaacc tgtcacatgt tattggcatg cagtcattca
     7741 tcgactcatg cctttcactg atatccctcc ctgtttatca ttaatttcta attatcagcg
     7801 tttttggctg gcggcgtagc gatgcgctgg ttactctgaa aacggtctat gcaaattaac
     7861 aaaagagaat agctatgcat gatgcaaaca tccgcgttgc catcgcggga gccggggggc
     7921 gtatgggccg ccagttgatt caggcggcgc tggcattaga aggcgtgcag ctgggcgcgg
     7981 cgctggagcg cgaaggatct tctttactgg gcagcgacgc cggtgagctg gctggtgcag
     8041 ggaaaacagg cgttaccgtg caaagcagcc tcgatgcggt aaaagatgat tttgatgtgt
     8101 ttatcgattt tacccgtccg gaaggtacgc tgaaccatct cgctttttgt cgccagcatg
     8161 gcaaagggat ggtgatcggc actacggggt ttgacgaagc cggtaaacag gcgattcgtg
     8221 acgccgccgc cgatattgcg attgtctttg ccgccaattt tagcgttggc gttaacgtca
     8281 tgcttaagct gctggagaaa gcagccaaag tgatgggtga ctacaccgat atcgaaatta
     8341 ttgaagcaca tcatagacat aaagttgatg cgccgtcagg caccgcactg gcaatgggag
     8401 aggcgatcgc ccacgccctt gataaagatc tgaaagattg cgcggtctac agtcgtgaag
     8461 gccacaccgg tgaacgtgtg cctggcacca ttggttttgc caccgtgcgt gcaggtgaca
     8521 tcgttggcga acataccgcg atgtttgccg atattggcga gcgtctggag atcacccata
     8581 aggcgtccag ccgcatgaca tttgctaacg gcgcggtaag atcggctttg tggttgagtg
     8641 gtaaggaaag tggtcttttt gatatgcgag atgtgcttga tctcaatagt ttg
//
LOCUS       DAZGJW010000178         8583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZGJW010000178 DAZGJW010000000
VERSION     DAZGJW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.178"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..859
                     /locus_tag="ACHKIK_004378"
     CDS             <1..859
                     /locus_tag="ACHKIK_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO9085429.1"
                     /translation="AIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGKSGAGK
                     SSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDAEYEAL
                     YRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPCHEWDM
                     AGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAAS
                     PLMTRLQDELCTESVRGQAREQFTGAVDRIFDTVESVCVASVARTVLRAVRDTVVSVA
                     RAVWNWIFF"
     gene            1231..4080
                     /gene="cah"
                     /locus_tag="ACHKIK_004379"
     CDS             1231..4080
                     /gene="cah"
                     /locus_tag="ACHKIK_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HFO9085430.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            4191..6575
                     /locus_tag="ACHKIK_004380"
     CDS             4191..6575
                     /locus_tag="ACHKIK_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO9085431.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRRAALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTSDLGW
                     ENYDEDVSRSVININKAREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCAFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            6572..7477
                     /locus_tag="ACHKIK_004381"
     CDS             6572..7477
                     /locus_tag="ACHKIK_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO9085432.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            7474..8543
                     /locus_tag="ACHKIK_004382"
                     /pseudo
     CDS             7474..8543
                     /locus_tag="ACHKIK_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
BASE COUNT         2107 a         2164 c         2511 g         1801 t
ORIGIN      
        1 tgccattgag gcaattgaaa aaccgctctc ctccctgcct tactcgcttt cccgtcacat
       61 cctggaacat ctgcgcaaac tcaccagtca cgaacccgtg attggcatta tgggtaaaag
      121 cggggccggt aaatcctcac tctgtaatgc actgtttcag ggggaggtca ccccggtcag
      181 tgatgttcac gccggcaccc gggaagtgcg gcgcttccgt ctgagtggcc atggtcacag
      241 catggttatc actgacctgc ccggggtggg cgagagccgg gacagggatg cagagtatga
      301 agccctgtac cgtgacattc tgcctgaact ggacttggta ctgtggctga ttaaagccga
      361 tgaccgtgcc ctgtctgtgg atgagtattt ctggcgacac atactgcacc ggggacatca
      421 gcaggtgctg tttgtggtga cgcaggccga caaaacagag ccctgccatg aatgggatat
      481 ggcaggtatt cagccttctc ccgcacagga acagaacatt cgcgaaaaaa cagatgcggt
      541 attccggctg ttccggcccg tacatccggt tgttgccgta tcggcccgca ccggctggga
      601 actggatacg ctggtcagtg cgctcatgac agcgcttccc gaccatgccg ccagtcccct
      661 gatgacccgc ctgcaggacg agctgtgcac ggaatctgtc cgggggcagg cccgtgaaca
      721 gtttaccggt gcggtggacc ggatatttga cacggtggag agcgtctgtg ttgcctctgt
      781 tgcacgcaca gttctgcgtg ccgtccgtga cacggtggtc tctgttgccc gcgcggtatg
      841 gaactggatt ttcttctgaa cagtctgcca ggggaaatgt ctgattacat tgcgttaacc
      901 aactgaaaac atgatacaaa cacctcatgc ccctcctgtt tctcttgcgt gactgctcta
      961 ctgttaatag aataaaacga tcgataaaac aggtatcgat cgtttatatc aatcgtttat
     1021 agtggttaat ggcctgcgaa aaggcagctg aatctcttca tcatgcagaa cggaattgca
     1081 cacaacagac tgatacttct ctgtctgatg aagggggaga gcgtttacgc ccgggacgta
     1141 tcgctgtgcc cgataactct gttttgtacc tgccggtatc cacttttgtg ggtaccggct
     1201 tttttattca ccctctgtaa ggaaaagctg atgaaacgac atctgaacac cagctacagg
     1261 ctggtatgga atcacattac gggcacccta gtggtggcct ccgaactggc ccgctcacgg
     1321 ggaaaacgcg ccggtgtggc ggttgcgctg tctcttgctg ctgtcacatc agtcccggca
     1381 ctggctgctg acaaggttgt acaggcggga gaaaccgtga acgatggaac actgacaaat
     1441 catgacaacc agattgtctt cggtacggcc aacggaatga ccatcagtac cgggctggaa
     1501 ctggggccgg acagtgaaga aaacaccggt gggcaatgga tacagaatgg cgggatagcc
     1561 ggaaacacca ctgtcaccac aaatggtcgt caggtcgtgc tggagggggg aacagccagt
     1621 gatacggtta ttcgtgacgg cgggggacag agcctgaacg gactggcggt gaacaccaca
     1681 ctgaataaca gaggcgagca gtgggtgcat gagggcgggg ttgccaccgg tacaattatc
     1741 aaccgcgacg gttaccagag cgttaaaagt ggcgggctgg caacaggaac catcatcaac
     1801 accggcgcag aaggcggccc tgattctgac aactcgtata cgggtcagaa ggtccaggga
     1861 acagcagaat ccaccaccat caacaaaaat ggacggcaga ttatcttatt ttccgggcta
     1921 gcccgtgaca ctctcattta cgcaggtggt gaccagtcgg tacacggaag ggccctgaat
     1981 accacactga atggcggtta ccagtatgtg cacagggacg gacttgcgct gaacacggta
     2041 attaacgagg ggggctggca ggttgttaag gcaggtggcg ctgccggtaa caccaccata
     2101 aatcagaacg gtgaactgag ggtacatgcc ggcggggaag ccactgcagt cacccagaac
     2161 acgggcggtg cactggttac cagtactgct gcaactgtca tcggcacaaa ccgtctgggg
     2221 aatttcacgg tggaaaacgg taaggctgac ggtgttgttc tggaatccgg cggtcgtctg
     2281 gatgtactgg agagccattc agcacagaat accctagtgg atgacggcgg taccctggca
     2341 gtgtctgccg gcggtaaggc gacaagtgtc accataacat ccggtggtgc cctgattgca
     2401 gacagtggtg ccactgttga ggggaccaat gccagcggta agttcagtat tgatggcaca
     2461 tccggtcagg ccagcggcct gctgctggaa aatggcggca gctttacggt taatgccggg
     2521 ggacaggctg gcaacaccac tgtcggacat cgtggaacac tgacgctggc tgccggggga
     2581 agtctgagtg gcagaacaca gctcagtaaa ggcgccagta tggtactgaa tggtgatgtg
     2641 gtcagtaccg gcgatattgt taacgcaggg gagattcgct ttgataatca gacgacaccg
     2701 aatgccgcgc tgagccgtgc tgttgcaaaa agtaactccc cggtaacgtt ccataaactg
     2761 accaccacga acctcaccgg ccagggcggc accatcaata tgcgtgttcg ccttgatggc
     2821 agcaatgcct ctgaccagct ggtgattaat ggtggtcagg caaccggcaa aacctggctt
     2881 gcgtttacaa atgtcggaaa cagcaacctc ggggtggcaa ccaccggaca gggtatccgg
     2941 gttgtggatg cacagaatgg cgccaccaca gaagaaggtg cgtttgccct gagtcgcccg
     3001 cttcaggccg gcgcctttaa ctacaccctg aaccgtgaca gcgatgaaga ctggtacctg
     3061 cgcagtgaaa atgcttatcg tgctgaagtc cccctgtata catccatgtt gacacaggca
     3121 atggactatg accggattct ggcaggctcc cgcagccatc agaccggtgt aaacggtgaa
     3181 aataacagcg tccgtctcag cattcagggc ggtcatctcg gtcacgataa caacggcggt
     3241 attgcccgtg gagccacgcc ggaaagcagc ggcagctatg gcttcgtccg tctggagggt
     3301 gacctgctca gaacagaggt tgccggtatg tctctgacga caggggtgta tggtgctgca
     3361 ggccattctt ccgttgatgt taaggatgat gacggttccc gcgccggcac ggtccgggat
     3421 gatgccggca gtctgggcgg atacctgaat ctggtacaca catcctccgg cctgtgggct
     3481 gacattgtgg cccagggaac ccgtcacagc atgaaagcgt catcggacaa taacgacttc
     3541 cgcgcccggg gctggggctg gctgggctca ctggaaaccg gtctgccctt cagtatcact
     3601 gacaatctga tgctggagcc acaactgcag tacacctggc agggactctc cctggatgac
     3661 ggccaggata acgccggtta tgtgaagttc gggcatggca gtgcacaaca tgtgcgtgcc
     3721 ggtttccgtc tgggcagcca caacgatatg acctttggtg aaggcacctc atcccgtgac
     3781 accctgcgcg acagtgcaaa acacagtgtg agtgaactgc cggtgaactg gtgggtacag
     3841 ccttctgtta tccgcacctt cagctcccgg ggtgacatga gcatggggac agccgcagcc
     3901 ggcagtaaca tgacgttctc accgtcccgg aatggcacgt cactggacct gcaggccgga
     3961 ctggaagccc gtatccggga aaatatcacc ctgggcgttc aggccggtta tgcccacagc
     4021 gtcagcggca gcagcgctga aggctataac ggtcaggcta cgctgaatat gactttctga
     4081 ttatacggta tcccggaaat acgatttcag gaaaattcat gcagtaatgg gaaaggacgc
     4141 tcattgctgt tcaccgcagt tctgaattat tgaatttata aaggtgaatg atgcacgaaa
     4201 agaacatcgc cctgctttgt gatgaagccg accggctttt gcaactgaat atcaatctgc
     4261 tccggcaaat ggttgatgag ccggatgtgt tatctgacag taagaacgaa aacgggctgc
     4321 tttttgataa acggaaagcg ctgaaaagaa ttgaggagct ggagggcgaa caaatcaaaa
     4381 ccgcccgcag ggagatggtg ctggctgtgg tcggcacgat gaaggcgggc aaatcaacca
     4441 ccatcaacgc cattgtgggg caggaaattc tgcccaaccg taaccgcccc atgacctcgg
     4501 taccgacatt gatccgccac gttcccggaa aaacggaacc ggtcctccat ctggaacata
     4561 ttcagcccgt ccgcaattta ttaatcacac tgcaggaaaa actcgccacc ccggcagggc
     4621 agcaagtcgc acagtccctg cagcaaaccg gggatacccg cgaactactg gatattctgg
     4681 cggatgatgt ctggcttaaa aatgaatacc acggggaaga cgaaatcttt accggactgg
     4741 catcgttaaa tgacctggtt cgtcttgctg cggcaatggg gactgaattt ccttttgacg
     4801 aatacgcaga agtgcagaaa ctaccggtga tcgacgtggc gttcagccac ctggtgggaa
     4861 tggatgcatg tcagggaaca ctcacactgc tggatacccc cggccctaat gaagccggac
     4921 aaccacagat ggaaatgatg atgcgggatc aactacagaa agcctctgcg gtgctggcgg
     4981 tgatggatta cacccagatg aactcgaaag ccgatgagga ggttcgtaaa gagcttaatg
     5041 ccattgctga cgtctcagcc ggtcgtctgt ttgtgctggt caataaattc gatgaaaaag
     5101 atcgcaatgg tgatggtgcg gatgccgtac gccagaaggt gccagcaatg ctgaacagcg
     5161 atgtgctgcc cgcctcccgc gtttatcccg gctcatcacg tcaggcgtac ctggctaacc
     5221 gtgcgcttca tgagttacga aaaaacggtg cgcttccggt tgatgaagcc tgggtcgatg
     5281 attttatcag ggaagctttc ggacctatgg tggaagagga cgactggaaa gacagcacta
     5341 aggtaaataa aaaggcagaa aaactctgga atatatctct gatcgatcaa ctgataacgg
     5401 aagtcataca gagctcccat tccagagcgg cggcactggc ggttgactct gccgccgcaa
     5461 aactgatgca gaatgcagaa aatgtcagcg aatatctgtc attacgtcat caggggctgc
     5521 agcaaagcat tcagtcacta caggcgcata ttaccagcct gctggcggat atccaggaga
     5581 ttgaggaatg tcagaatcag gtgaccggtg atgtcagaat ggccatggag gacatcaata
     5641 ccaaaaccgg ggaattactg acgaaggtct gtgcctcact ggaggaggag ctgaacgact
     5701 atttcagaag cggtaaacgc aaagaacagc aaatgctgga ggaagaaaat tcagcgcagc
     5761 cccgggagag aaacgcattc gctttctttc acgatatttt cggaaccgga aatcagcatg
     5821 acaggatgcg ggactttgat ccggacagcc ccgaaataaa attcagtgat cgcagggcag
     5881 cccttgaact gatgacgcaa atcgaatcga ccgtgaccag cctgcaccgt gaggctgaag
     5941 cacagttccg gcctgagctg gagaaaatcg tcagcgggat tgaaacaggt tttcgtggca
     6001 cggccctgta cgccacagaa aacattgccg gtcgcatcaa tgcccgcctg gaggatgagg
     6061 gctttaccgt aaaaatcagt tttccggcag tcagccagtt acagacccgg ctcgcggtaa
     6121 aaataaatct gagtgcgctt atggaggaaa gaacggagac cgtcacccgt cgccgtcggc
     6181 agagtggcgt atggggaacc gtttgtcgat ggtttggcac cagtgacctg ggctgggaaa
     6241 actatgacga ggatgtgagt cgcagcgtga tcaatatcaa caaggccaga gaggaagtta
     6301 tgtcactgac ccgggcatat ttcggggagc tgcaggcatc cattgagcag gatattaacc
     6361 agcccgtccg ccaggaaatc gatgcctttt tctgcgcatt cagggagaaa gttgaacaac
     6421 tgcgtaacac gctgattcag agctctgaag atcataaacg cgatcagcag gcgcaggaac
     6481 ggcttaccgg gcgacttcag gcattaaatg aaagggttcc tgagctcatt actgacagta
     6541 aggcgctgag ggaagagctg gagacaatgc tgtgacttca ccatttattc aacaaattgc
     6601 tgataaccgg gtatgtcagg tactcacctg tcttcctgaa aaatttgtgg ttgatttcgc
     6661 aaacggaatc gatgttgcac aggagcatat ccgcacggcc ggagagcgca cgttcttccg
     6721 gcgtttaaaa gaggggctga ccggcgaagg cgctgcacgg cagaatgcca tcaatgcgtc
     6781 gcttgcacag ggcgttgagg cgtccctgcg ctggctgacg gagatgacaa cgtcacttgc
     6841 caccacaaac tacgcgatta cccgggtaaa cgacagggtc agttcactgg tcagtgatac
     6901 agccaggctg gcgcattatt ccgcagatac gcgggagcag ttactcaccc tggccgatca
     6961 ggttcaccat aaactgaatc atcttgaaga aaaactccac cgtgttgacc aggttcagcg
     7021 ggcgcagtta catcttgagc agatattctc atggtggagc gccgggcgat atgcgtcatt
     7081 ttcccctgcc ggacgttgtt atgtggcact tgaagagctt cgctggggcg cgtttggtga
     7141 tgtgatacgt cagggcgaaa caggccaggt taaccagcta ctggatattc tcagacataa
     7201 agcattaacg cagatggcac aggagagtgg cggtagtgca accgtgcgtc tgaacactct
     7261 ggactggctt ggtggtcagg gccgggagca ggcagataac gagtggcatg acgcgattaa
     7321 ctggctggga gactggtgca gtgaagagca gcatccggtg atctggtcaa ccacgcaggc
     7381 tgcagaacac ttaccggttc gcatgcctcg cctctgctct gcagaacgcc tctctgaaag
     7441 tatggtcgat gaaatatttc agaagggggc tgcatgagta cagaaatgaa aacggggctg
     7501 gtgctgtccg gtggcggggc ggtgggcgct tatcaggcgg gagtggttaa ggcactggca
     7561 gagtgtggta cacagatcag catggtttca gggaccagca ttggcgcatt caatggtgcc
     7621 attatcgcgg cctctcccga tctgtcagaa gctgccgtac gcctggaggc gctctgggag
     7681 catctgggga ataatcaggt gctgtcggta aacagattgg tttacttttc attgctgaaa
     7741 aaattgttcc aggcaatgaa cctctgccag atccccggac gtgcaggagc actgcttacg
     7801 acgcttcttc gccatatatc gacaatcaac gggtttgaca atctgatggc tcagccgttg
     7861 ttgtcagatg agcccctgac agcgctgatg gatcattatc ttgatactga tgctctggca
     7921 gacgggctac cgctgtatgt gtcgctgtac cccacagaag gggcatgcag gatattattg
     7981 actgcattcg tgctgaactg ggtgtcggaa ccacgaaaaa cgccgttttt cagcatatcc
     8041 agagcctgcc ccgcggacag cagaaagagg ctctgcttgc gtcagccgcg ctgcccctgc
     8101 tgttccgtcc ccgtgaggtt caggggacaa tgttcggtga tggtggtatg ggaggatggc
     8161 gaaatatgca gggaaatacc cctgtgacgc ctctggtcga tgccggatgc aatatggtga
     8221 ttgtgacgca tctgagtgac ggttctttat gggatcgcca ggcttttccg gacaccacaa
     8281 tccttgagat ccgtccccgg aaaaggctga aatatgcagg tgatggtggc aacagcggcg
     8341 gtctgctcag ttttacatcg gcacataccg acgcctggcg tcagcagggc tatgaagaca
     8401 cgatgctggc gatggagcat atccggaaac cgctggcagc acgtcaggca ctgacccggt
     8461 cagaggcggt attgcagaaa agcctggata taacggaaga ggcagatttg gcactgagaa
     8521 acgcgatggc ccggattaaa taaagacgcc ccaaagaaac gccaccgcct gtggtggtgt
     8581 ttt
//
LOCUS       DAZGJW010000179         8539 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZGJW010000179 DAZGJW010000000
VERSION     DAZGJW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.179"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            299..1276
                     /gene="glaH"
                     /locus_tag="ACHKIK_004383"
     CDS             299..1276
                     /gene="glaH"
                     /locus_tag="ACHKIK_004383"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO9085433.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1296..2564
                     /gene="lhgO"
                     /locus_tag="ACHKIK_004384"
     CDS             1296..2564
                     /gene="lhgO"
                     /locus_tag="ACHKIK_004384"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO9085434.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2587..4035
                     /gene="gabD"
                     /locus_tag="ACHKIK_004385"
     CDS             2587..4035
                     /gene="gabD"
                     /locus_tag="ACHKIK_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO9085435.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPMVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4049..5329
                     /gene="gabT"
                     /locus_tag="ACHKIK_004386"
     CDS             4049..5329
                     /gene="gabT"
                     /locus_tag="ACHKIK_004386"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO9085436.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5640..7040
                     /gene="gabP"
                     /locus_tag="ACHKIK_004387"
     CDS             5640..7040
                     /gene="gabP"
                     /locus_tag="ACHKIK_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO9085437.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPALMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7061..7723
                     /gene="csiR"
                     /locus_tag="ACHKIK_004388"
     CDS             7061..7723
                     /gene="csiR"
                     /locus_tag="ACHKIK_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO9085438.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7724..8173)
                     /gene="kbp"
                     /locus_tag="ACHKIK_004389"
     CDS             complement(7724..8173)
                     /gene="kbp"
                     /locus_tag="ACHKIK_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO9085439.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8257..8415)
                     /gene="yqaE"
                     /locus_tag="ACHKIK_004390"
     CDS             complement(8257..8415)
                     /gene="yqaE"
                     /locus_tag="ACHKIK_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO9085440.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         1922 a         2229 c         2378 g         2010 t
ORIGIN      
        1 aatatgttta gtttactaac ggtcattttg cagtgaagcc atttactgtt ttttatcgac
       61 cagataatct gttctctaat gttaactccc cctaacctgt tgctttagtt attcatttcc
      121 tgtctcactt tgccttaata ccctacgtta aatgttacta atttgttgct tttgatcaca
      181 ataagaaaac aatatgtcgc ttttgtgcgc atttttcaga aatgtagata tttttagatt
      241 atggctacga aatgagcatc gccatgtcac cctacatctc ataagaggat cgcttctgat
      301 gaatgcactg accgccgtac acaataacgc tgtcgattca ggccaggact atagcggatt
      361 cactctcatc ccgtcggcgc aatccccgcg tctgctggaa ctcaccttca ccgaacagac
      421 gaccaaacag tttctcgaac aggttgccga atggcccgtg caggcgctgg agtacaaatc
      481 gtttctgcgt tttcgggtag gcaaaattct tgacgatctg tgtgcgaatc agctgcaacc
      541 attgctgttg aagaccctgc taaaccgtgc tgaaggtgcg ctgttgatca atgcggtggg
      601 tatcgatgat gtggcgcagg cagatgagat ggtgaagctg gcaacggcgg tagcgcatct
      661 gattggtcgc tcaaatttcg atgcgatgag cggtcagtat tacgcgcgtt tcgtggtgaa
      721 aaatgtcgat aactcagaca gctatctgcg tcagccgcac cgcgtgatgg aactgcacaa
      781 cgacggcacg tatgtcgaag agatcaccga ttacgtgctg atgatgaaaa tcgatgagca
      841 aaacatgcag ggtggaaatt cgttgctgct gcatctcgat gactgggaac atctggacca
      901 ctatttccgc catccactgg cgcgtcgccc gatgcgcttt gccgcgccgc cgagcaaaaa
      961 cgtcagcaaa gatgttttcc atccagtgtt cgacgttgat caacagggtc gcccggtgat
     1021 gcgctatatc gaccagttcg tccagccaaa agattttgaa gaaggcgtgt ggttgagcga
     1081 gctttccgat gccattgaaa ccagcaaagg cattctttct gtacccgttc ccgttggcaa
     1141 attcctgttg ataaacaacc tgttctggct gcacggtcgc gaccgcttta ctccgcaccc
     1201 ggatctgcgc cgtgaactga tgcgtcagcg tggctatttc gcttacgcca ctcaccacta
     1261 ccagacgcat cagtaagcgc aaaggaattg agcggatgta tgattttgtg attattggcg
     1321 gcggcatcat cggcatgtcg accgccatgc aactgattga tgtttatccg gatgcccgca
     1381 ttgcgttgct ggaaaaagag tccggcccgg cctgtcacca gacgggccac aacagcggcg
     1441 tgatccatgc cggggtctat tacacgcccg gcagcctgaa ggcgcagttt tgcctggcgg
     1501 gaaaccgcgc caccaaagcc ttttgcgatc aaaacgacat tcgctatgac aactgcggca
     1561 agatgctggt cgccacgtcc gatctcgaaa tggaacggat gcgcgcgttg tgggaacgca
     1621 cggcggcgaa cggtatcgag cgcgagtggt taaacgccga ggaactgcgc gagcgcgaac
     1681 cgaatatcac cgggctcggc ggcatttttg tgccgtccag cggcattgtc agctatcgcg
     1741 aagtcacagc ggcaatggca aaaatcttcc aggccagagg cggtgagatt atttataacg
     1801 ccgaagtcag cgccctcagt gagcataaaa acggcgtggt cgtccgaacc cgtcagggcg
     1861 gcgaatatga agcatcgacg ctgattagct gttccgggct gatggctgac cggctggtga
     1921 aaatgctcgg cctcgaaccg ggttttatta tctgcccgtt ccgtggcgag tatttccgcc
     1981 ttgcgccgga gcataaccag attgttaacc atctgattta ccccattccc gacccggcaa
     2041 tgccattttt gggcgtgcat ctcacccgaa tgatcgatgg cagcgtcacc gtcgggccaa
     2101 acgcggtgct ggctttcaaa cgcgaaggct atcgcaagcg cgacttctca tttagcgaca
     2161 cgctggagat tttgggctcg tcggggattc gccgggtgct gcaaaaccat ctacgctcag
     2221 gactgggcga gatgaaaaac tcgctgtgca aaagcggcta tctgcgcctg gtgcaaaagt
     2281 attgtccccg gctttcgtta agcgatctcc agccctggcc cgccggtgtg cgggcgcagg
     2341 cagtatcgcc ggacggcaag ctgattgacg attttctgtt tgtcaccacc ccacgcacga
     2401 tccacacctg caatgcgccc tccccggcag cgacatcagc aattcctatt ggtgcgcata
     2461 ttgtcagcaa ggtacaaacg ctgttggcaa gccagagtaa ccccggacgc acgctgcgag
     2521 cggcacgtag tgtggatgcc ttacacgccg catttaatca ataacctttg aaaacaggac
     2581 gtaacaatga aacttaacga cagtaactta ttccgccagc aggcgttgat taacggggaa
     2641 tggctggacg ccaacaatgg tgaagtcatc gacgtcacca atccggcgaa cggcgacaag
     2701 ctgggtagcg tgccgaaaat gggcgctgat gaaacccgcg ccgctatcga cgccgccaac
     2761 cgcgccctgc ccgcctggcg tgcgctcacc gccaaagaac gcgccaacat tctgcgcaac
     2821 tggttcaatt tgatgatgga gcatcaggac gatttagcgc gcctgatgac cctcgaacag
     2881 ggcaaaccac tggccgaagc gaaaggtgaa atcagctacg ccgcctcttt tattgagtgg
     2941 tttgccgaag aaggcaaacg catttatggc gacaccattc ccggtcatca ggccgataaa
     3001 cgcctgattg ttatcaagca gccgattggc gttaccgcag ctatcacgcc gtggaacttc
     3061 ccggcggcga tgattacccg taaagccggt ccggcgctgg cggcaggctg caccatggtg
     3121 ctgaagcccg ccagtcagac accgttctct gcgctggcgc tggcggagct ggcgattcgc
     3181 gcgggcattc cggctggcgt gtttaacgtg gtcaccggtt cggcaggcgc ggtcggtaat
     3241 gaactgacca gcaacccgat ggtacgcaaa ctgtcgttta ccggttcgac cgaaattggc
     3301 cgccagttaa tggaacagtg cgcgaaagac atcaaaaaag tgtcgctgga gctgggcggc
     3361 aacgcgccgt ttatcgtctt tgacgatgcc gatctcgata aagccgtgga aggcgcgctg
     3421 gcctcgaaat tccgcaacgc cgggcaaacc tgcgtctgcg ccaaccgttt atacgtgcag
     3481 gacggcgtgt atgaccgctt tgccgaaaaa ttgcagcagg cggtgagcaa actgcacatc
     3541 ggcgacgggc tggataaagg cgtcaccatc gggccgctga tcgatgaaaa agcggtagca
     3601 aaagtggaag agcatattgc cgatgcgctg gagaaaggtg cgcgtgtggt ttgcggcggt
     3661 aaagcggatg aacgcggcgg caacttcttc cagccgacca ttctggtgga cgtgccggcc
     3721 aacgccaaag tgtcgaaaga agagacgttc ggccccctcg ccccgctgtt ccgctttaaa
     3781 gatgaagccg atgtgattgc gcaagccaat gacaccgagt ttggccttgc cgcctatttc
     3841 tacgcccgtg atttaagccg cgtcttccgc gtgggcgaag cgctggagta cggcatcgtc
     3901 ggcatcaata ccggcattat ttccaatgaa gtggccccgt tcggcggcat caaagcctcg
     3961 ggtctgggtc gtgaaggttc gaagtatggc atcgaagatt acttagaaat caaatatatg
     4021 tgcatcggtc tttaactgga gaatgcgaat gaacagcaat aaagagttaa tgcagcgccg
     4081 cagtcaggcg attccccgtg gcgttgggca aattcacccg attttcgctg accgcgcgga
     4141 aaactgccgg gtgtgggacg ttgaaggccg tgagtatctt gatttcgcgg gcgggattgc
     4201 ggtgctcaat accgggcacc tgcatccgaa ggtggtggcc gcggtggaag cgcagttgaa
     4261 aaaactgtcg cacacctgct tccaggtgct ggcttacgag ccgtatctgg agctgtgcga
     4321 gattatgaat cagaaggtgc cgggcgattt cgccaagaaa acgctgctgg ttacgaccgg
     4381 ttccgaagcg gtggaaaacg cggtaaaaat cgcccgcgcc gccaccaaac gtagcggcac
     4441 catcgctttt agcggcgcgt atcacgggcg cacgcattac acgctggcgc tgaccggcaa
     4501 ggtgaatccg tactctgcgg gcatggggct gatgccgggt catgtttatc gcgcgcttta
     4561 tccttgcccg ctgcacggca taagcgagga tgacgctatc gccagcatcc accggatctt
     4621 caaaaatgat gccgcgccgg aagatatcgc cgccatcgtg attgagccgg ttcagggcga
     4681 aggcggtttc tacgccgcgt cgccagcctt tatgcagcgt ttacgcgcgc tgtgtgacga
     4741 gcacgggatc atgctgattg ccgatgaagt gcagagcggc gcggggcgta ccggcacgct
     4801 gtttgcgatg gagcagatgg gcgttgcgcc ggatctcact acctttgcga aatcgatcgc
     4861 gggcggcttc ccgctggcgg gcgtcaccgg gcgcgcggaa gtgatggatg ccgtcgctcc
     4921 aggcggtctg ggcggcacct atgccggtaa cccgattgcc tgcgtggcgg cgctggaagt
     4981 gttgaaggtg tttgagcagg aaaatctgct gcaaaaagcc aacgatctgg ggcagaagct
     5041 aaaagacgga ttgttggcga tagccgaaaa acacccggag atcggcgacg tacgcgggct
     5101 gggagcgatg attgccatcg agctgtttga agatggcgat cacagcaaac cggacgcaaa
     5161 actcaccgcc gagatcgtgg cacgcgcccg cgataagggc ctgattcttc tctcctgcgg
     5221 cccgtattac aacgtgctgc gcatccttgt accgctcacc attgaagacg ctcagatccg
     5281 tcagggtctg gagatcatca gccagtgttt tgctgaggcg aagcagtagc tatgtccgtt
     5341 tgtaagagtt aaacgttgct aagggaatga taacgatgca gtaaaaaata ataacgatga
     5401 ggcgatcgac aaagttaaaa acgactgcgg actgtcccct cgctcctctg gggagagggt
     5461 tagggtgagg ggtaatcacg cggctacacc ctcaccccgg ccctctccct aagggagagg
     5521 gagaaaactc tcccgctttc gtttttaaga ttttgttgac cgtctcaatt aaacgcgtga
     5581 cccgacgcgg gaaatgtcgg ggcgctctcc caagtgacac actttcgaga ggattcagga
     5641 tggggcaatc atcgcaacca catgagttag gcggcgggct gaagtcacgc cacgtcacca
     5701 tgttgtctat tgccggtgtt atcggcgcaa gtctgtttgt cggttccagc gtcgccatcg
     5761 ccgaagcggg cccggcggta ttactggcct atctgttcgc cggattactg gtggttatga
     5821 ttatgcggat gttggcggaa atggcagttg ccacgcccga taccggttcg ttttccacct
     5881 atgccgataa agccattggc cgctgggcgg gttataccat cggctggctg tactggtggt
     5941 tttgggtact ggttatcccg ctggaagcca acatcgccgc tatgatcctg cactcgtggg
     6001 ttccaggcat tcccatctgg ttattttccc tcgtcattac cctcgcctta actggcagta
     6061 atttattaag cgttaaaaac tacggcgaat ttgagttctg gctggcgctg tgcaaagtca
     6121 tcgctatcct ggcctttatt ttccttggtg cagtcgcaat tagcggtttt tacccttatg
     6181 ccgaagtgag cgggatctca agattgtggg atagcggcgg ctttatgccc aacggcttcg
     6241 gtgcggtatt aagcgcgatg ttgatcacca tgttctcgtt tatgggcgca gaaattgtca
     6301 ccattgccgc cgcggaatcc gacacgccgg aaaaacatat tgtccgcgct accaactcgg
     6361 ttatctggcg tatttctatc ttctatttgt gctctatttt tgtcgtagtg gcgttaatac
     6421 cgtggaatat gccggggctg aaagccgttg gttcttatcg ctcggttctg gaattgctca
     6481 atattcccca tgcgaaatta atcatggact gcgtgatatt actttccgta accagctgtc
     6541 tgaactcggc gctgtatacc gcgtcaagga tgctctactc cttaagccgt cgcggtgatg
     6601 cgcccgcgct aatgggcaaa atcaaccgca gtaaaacccc gtatgtggcg gtgttactct
     6661 ccaccggagc ggcattttta acggtggtgg tgaactatta cgcacctgcg aaagtgttta
     6721 aattcctgat agacagctcc ggcgctatcg ccctgctggt ttatttagtc atcgccgttt
     6781 cacagttgcg gatgcgcaaa attctgcgag cagaaggaag cgaaattcgc ttgcgcatgt
     6841 ggctttatcc ctggctcacc tggctggtca tcggctttat tacctttgtg ttggtagtga
     6901 tgctattccg tccggcgcaa cagttagaag tgatctccac cggcttatta gcgataggga
     6961 ttatctgtac cgtgccaatt atggctcgct ggaaaaagct ggtattgtgg caaaaaacac
     7021 ccgttcataa tacgcgctga taatgatcag gagtcacacc atgaccatta cgtctctgga
     7081 tggctatcgc tggctgaaga acgatattat tcgcggtaat tttcaaccgg atgaaaaatt
     7141 acggatgagt ttgctgacat cgcgttatgc acttggcgtt gggccgttgc gggaagcact
     7201 ttctcaactg gtggcggaac ggctggtcac ggtggtgaat caaaaagggt atcgggtggc
     7261 gtctatgtca gagcaggagc tgctcgatat tttcgacgcc cgcgccaata tggaagcgat
     7321 gctggtcagt ctggcgattg cccgcggtgg cgatgaatgg gaggcagacg ttctcgcaaa
     7381 agcgcatctg ctcagtaagc tggaagcctg tgacgccagc gagaaaatgc ttgatgagtg
     7441 ggatctgcgt catcaggcgt ttcatacagc aattgtggcg ggctgtggtt ctcactattt
     7501 gctgcaaatg cgtgaacggt tgtttgatct ggcggcgcgc tatcgattta tctggctgcg
     7561 gcgaacggtg ctttcggtag aaatgctgga ggataaacac gatcagcacc agaccctgac
     7621 tgcggcggta ctggcgcgag ataccgcgcg cgccagtgag ttaatgcgcc agcatttact
     7681 gacgccaatt cccattatcc agcaggcgat ggctggcaat taactactct tccggaatac
     7741 gcaacacttg ccccggataa attttatccg ggctttttag catcggttta ttcgcttcga
     7801 agattttatt gtacagatta gcgttaccgt agacctgttt ggaaatggca ctcagagtgt
     7861 cgccagactt aacggtataa aactggctgg cagtggctgg tgtcgccgtt ttcacctgat
     7921 catcgacact ggcaataccg gaaatattcc caaccgcaac aaggattttc tccttcgcct
     7981 cctgactcag gccgtcacca gtgaccgtcg ctttgccgtc ggcaatttga atgttcactt
     8041 tatcggcatc aggtataccg gttttgctca gatgctcctg caccttcttc gcctgatcgt
     8101 ctttatcgtg ctgacctgta accgcgtccc agagtttttc tccggcatct ttcacaaaat
     8161 tgaacagacc catagctacc tcattcgtta acggaaacac tcagaaagtg tagcagagga
     8221 acgcggcgcg ttaacgccgc gggggtgagc acaggtttaa tcgcgggttt gcacccagaa
     8281 cgcgtgaatc aaaccgggga tatacccaag cagtgtcagc agaatattaa taatgaacgc
     8341 ccaaccgaac cctttaccga gcagcacgcc gagcggcggc agaatgatgg tgatgacgat
     8401 tctccagaaa cccatatgta ctccctataa gaaaattact cattgtttaa aaagagattt
     8461 tatctcttaa gcgtagtaat tttagcggag cctgccagtt tcagcttacg cttttactct
     8521 catttacgct gctgaggct
//
LOCUS       DAZGJW010000180         8408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZGJW010000180 DAZGJW010000000
VERSION     DAZGJW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.180"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..567)
                     /gene="yeeO"
                     /locus_tag="ACHKIK_004391"
     CDS             complement(<1..567)
                     /gene="yeeO"
                     /locus_tag="ACHKIK_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO9085441.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAVAITLIGSGALRGAGN"
     gene            728..803
                     /locus_tag="ACHKIK_004392"
     tRNA            728..803
                     /locus_tag="ACHKIK_004392"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:761..763,aa:Asn,seq:gtt)
     gene            complement(841..1791)
                     /gene="cbl"
                     /locus_tag="ACHKIK_004393"
                     /pseudo
     CDS             complement(841..1791)
                     /gene="cbl"
                     /locus_tag="ACHKIK_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
     gene            complement(1893..2810)
                     /gene="nac"
                     /locus_tag="ACHKIK_004394"
     CDS             complement(1893..2810)
                     /gene="nac"
                     /locus_tag="ACHKIK_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO9085442.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3136..3211
                     /locus_tag="ACHKIK_004395"
     tRNA            3136..3211
                     /locus_tag="ACHKIK_004395"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3169..3171,aa:Asn,seq:gtt)
     gene            complement(3255..4202)
                     /gene="ldtA"
                     /locus_tag="ACHKIK_004396"
     CDS             complement(3255..4202)
                     /gene="ldtA"
                     /locus_tag="ACHKIK_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO9085443.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMFYERW"
     gene            complement(4264..5343)
                     /gene="cobT"
                     /locus_tag="ACHKIK_004397"
     CDS             complement(4264..5343)
                     /gene="cobT"
                     /locus_tag="ACHKIK_004397"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO9085444.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCAAHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(5355..6098)
                     /gene="cobS"
                     /locus_tag="ACHKIK_004398"
     CDS             complement(5355..6098)
                     /gene="cobS"
                     /locus_tag="ACHKIK_004398"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO9085445.1"
                     /translation="MSKLFWAMLSFITRLPVPRRRSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6095..6640)
                     /gene="cobU"
                     /locus_tag="ACHKIK_004399"
     CDS             complement(6095..6640)
                     /gene="cobU"
                     /locus_tag="ACHKIK_004399"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO9085446.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(6792..6965)
                     /locus_tag="ACHKIK_004400"
                     /pseudo
     CDS             complement(6792..6965)
                     /locus_tag="ACHKIK_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(6944..7132)
                     /locus_tag="ACHKIK_004401"
                     /pseudo
     CDS             complement(6944..7132)
                     /locus_tag="ACHKIK_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(8129..8408)
                     /locus_tag="ACHKIK_004402"
                     /pseudo
     CDS             complement(8129..8408)
                     /locus_tag="ACHKIK_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2271 a         2243 c         1807 g         2087 t
ORIGIN      
        1 attccctgca ccacgaagtg ccccgctacc aatcaaagtg atggcaactg ctggataact
       61 gagtaccgtc agctccagat aagtcaacgc cagtgcttta acttctgtcg tggcatcacc
      121 cgcgacgaaa tcaataattt gttcgccaaa atgatgaata agcgttgcca acagtacggc
      181 aaacaacgtc atgatcacca atgactgccg cgtcgccacc ctcgctcgtc gtcgatcccg
      241 cttaccgaga ctaaatgcca caacgacagt agtaccaaga tcgatagcag caaaaaaagc
      301 cataatgacc atattgaagc tgtccgccaa tcccacgccg gccatcgcat cttttcccag
      361 ccagctgacc agaaaagtgc tcaggacacc catcaacagg acacaggcat tctccatgaa
      421 gataggaaca gcaagcgggg ttatctcgcg ccagaacaac actttgtagc tcttgcgttt
      481 agcgtgccag cgagtgccgt gaacaacctg gcgtaaagca gaggagatat tcaaagccga
      541 ccttaattgc agaaagtgaa accacatttc aaataatgag ggagaatcag caaagctgca
      601 aagattttcg ccaacaaatt gtctgcaaat gcaacaaact gttgatagaa acggcaaaca
      661 gttgggaaat ttaaaaatcg ggtttgacaa aagatttttc gccgttaaga tgtgcctcaa
      721 cagcgattcc tctgtagttc agtcggtaga acggcggact gttaatccgt atgtcactgg
      781 ttcgagtcca gtcagaggag ccaaatttct gaaaagcccg cttttatagc gggctttttg
      841 ctatatctga taatcaattt cctcttcact gttctccatc acctggcgct tgatatcctc
      901 aactgacagt cctgcattac aaagttccag aaagcgccag acatagttac gttgaagttg
      961 ccctcgcttc agtcccaacc atacagtatt ggcatcgaaa agatgccgcg tatccagacg
     1021 gattaaattc tcttcctctt gttcgccgct ggattgttcg gcaactaatc caatccccag
     1081 cccaagagca acataggttt taatgacatc agaatcctgc gcacttaata caatatctgc
     1141 cagcaaacct ttgcgggcaa atgcgtcatc aatacgtgag caccccgtaa tcccctatcg
     1201 gtaagtgatt aacggccact tcgctattga ttccagcgtc aacggtgtaa tttgcgtcaa
     1261 gggatgatcg agtggaacaa gcaaactatg gtgccaacga aaccacggga aggcgacgag
     1321 atgcgggtca ttactcaaac gctcgctggc gataccaata tcagcttcgc cattttgcaa
     1381 caacgtcgca atttcctgtg gcgtaccctg gattagctcc agccgaacct ccgggaaaag
     1441 ttcgcgaaaa gctttaatga cctcaggcaa gctataacgt gcctgagtat gcgtcgttgc
     1501 aatagtgaga acgccagacg tatcgttggt aaacaggtct gcaagccgac gaacattact
     1561 ggcttcattc agaatacgtt ctgcaatgac cagtaatgct ttgcccggtt cagtcatacc
     1621 cagcagtcgc ttacctcgtc gaacaaatat ttcgatgcca agttcatccc ccagttcccg
     1681 aatatgacgg ctgacgccgg actgtgaggt aaaaagcata ttcgcaacct ctgtcaggtt
     1741 gtaatcctga cgtgcagcct cgcggattat ctttagttgt tggaaattca cggtaaactc
     1801 cgggcagctc agatttcccg ttattgttaa agtctaatgc ccggcataac aaataataaa
     1861 aacccgcatc ttattccatc ccgatataac gcttagctca ccaattgcca ctgccttttt
     1921 tccatcactg gagaactaat cactgacatt aacaactctt tcactgcctg tgcctgtggc
     1981 gataagttcg ctctggcggg taaatttaat gacaaagaga gactcatgga aggcgtggta
     2041 atgcgtgaca tccacccatt tactgcgcca cataacgaac gcgcggccga ttcgggtaat
     2101 actgcaacgc ccatgccgct ggcaatcgct gcggtaagcg tggcaataga ctcaatttca
     2161 ccaatgactt ttgccgtgag ccgccgtagg gaaaaagcct catcaacacg aagtctaaca
     2221 gcactgtaat cactggggag aaagaggttc atttgcgcaa tagcattaac atcaacgctt
     2281 tgccccgggc aatcttgagt tcctaccagg aaaagatctt ctttcagcaa agcctgactg
     2341 gataccccag ccacagggga atgctcataa atcaccgcca tatcgagttg gtgatttatc
     2401 agtttttcgt taagcactgc accgctattt tcatgaagat agataacgat ctccgggaat
     2461 tcagcgcgaa ccgcttgtaa taagggcatg gtgatagatg acgcagcggt tcctggcgca
     2521 aagccaatcg agacttgccc cgataatgcc tgaccaacgt tatgcaccgc cagttgggcc
     2581 tgctcacact gacgtaaaat ggcccgcgca tgggtataga gaatttttcc ggcgtctgtt
     2641 ggcgtaacgc cccgttttgt gcgaatcaaa agttgttgat ttaactcacc ttccagtgtg
     2701 gcaacctgct ggctgagcgc tggttgtgcg atatgcaata cttcagcagc ctgagtcagg
     2761 ctaccaatat ctacaatttt tacgaagtat ttcaggcgtc tgaagttcat gttgcctccg
     2821 gtttttgaga atcggcccaa gtgccgccat tacttacaac cagactgcaa gatgcttgcc
     2881 agttttattt tggcgttgat gtacaagcta accaactgtc aaataagaga ttatgataga
     2941 ttcgtcattg gctcttttca ccagcagtcg cgttcccctg ccctataaaa ggagggtatg
     3001 caccacgatg gttcattacc aataagattg aaatctcatc actttgttga aattggcagc
     3061 aaacaaacaa aaaaatgcat ttcacccttt gacatcacca tgcactgcca ttaatatgcg
     3121 ccccgttcac acgattcctc tgtagttcag tcggtagaac ggcggactgt taatccgtat
     3181 gtcactggtt cgagtccagt cagaggagcc aaattcaaaa aagcctgctt tctagcagga
     3241 tttttgcttt ctaattacca acgctcataa aacatctgtc ttgaaccaga actaatttgc
     3301 acaggcattc ccgatcgacg ttgcaacgca gcatttgcgc gattcacatc aacttcttgc
     3361 ccgttgataa acgcccgcaa agatggggtt accggcaatg gcacttttcg gtcagactca
     3421 tattctgcac gattgcgcga cagcggctca tgaacttcca gccagttcga gccatctggt
     3481 tcagtggtgt attttactgg ctggtcgata atttgcacac gcgttccaac aggaacatta
     3541 tcaaacagat atttgatatc gtcattgcgc agacgaatac agccctgact tacccgaagt
     3601 ccaataccaa aattggcatt ggtaccgtgg atggcataca acctgccaat ataaatcgcg
     3661 tacagcccca tgggattatc gggccccgca ggaacaaatg cgggcaaact ctcccctcgt
     3721 ttcgcatatt cgcgccgagt gttcggcgtt ggcgtccagg ttggagcttc ttgtttacgt
     3781 tcaacggtag tcacccagtt acgcggggtt tctcgcccag cctggccgat accaatagga
     3841 aagacttcca cagtattact gtctggcggg tagtaataaa gacgcatctc agcgacgtta
     3901 accacaatcc ctttacgaac agtggcgggc aaaatcagtt gctgcggaat ggtgagttgc
     3961 gagccagact tcggcaagaa aacatcagcg cccggattcg cttccagcat gttacttaac
     4021 ccttgcccgt attgtgcggc aaaagtctcc agcggctggg tattgtgatc aggaacagtt
     4081 acagtaagcg actgccccac caaacggcta ccctctggag gtaatggata agttaccgcc
     4141 aggctagtat ggctggcaaa aagcagagca aatgagcaaa gaatatttac acgacgcatc
     4201 atgtcccttt cctatgtcgc gaaagctatc cgttaagtat agcttttatc agacttttcg
     4261 tttttaactg ttcaaatcag aagtcgtatt ccccggtaga acaatattac tggcagcaag
     4321 ttcgcccatg ttgttatata tcgcacaggc agcttcgatg atgggcatcg ccagagctgc
     4381 gccactcccc tcacctaaac gcatctccat attgagataa ggctccagcc ccaaatgcga
     4441 cagcgctata cgcgcccctt tttctgccga caggtgagaa ggaatgagat acggtttgat
     4501 tgcaggagac atctggcagg ctgcgagcgc agcagcataa gaaagaaatc catccagcaa
     4561 cacgggtaaa ccgcaggaag cagcgcctaa catcacgcca gccattccga ccaaatcaaa
     4621 tccccccact tttgccagaa catcaacgcc atcctgcgga tttggttgat tcaacgtaat
     4681 cgcccggcgc acaacatcaa ttttattagc cagtttatct gtcggcaggt ttgcgccaat
     4741 cccaaccact tcttcaggat cccggccagt gattgtgctg actattgccg ctgccggtgt
     4801 cgtgtttgcc atccccagtt cacctacacc aaacagcgtg acaccgtttt ttgccagctc
     4861 ccgcgtataa catatgacgt ccaaaagcaa cttttcagcc tgacggcgac tcattgccgg
     4921 agctgaagca atattgccgc tacctcgtgc gacacgcatg ttgataagcc cggggatagg
     4981 ctcagcacta tcaataccaa catcaactac gtggacgtta gcgcccgctt gtgctgccag
     5041 cacacacacg ccggttgttc cgcgggtcat attttcagcc tgaatggctg tcacttcttt
     5101 tggggaaata gcgacccctt cctcccagac gccgtgagcg gcacacataa ccagtaccgc
     5161 ttttttgccc acatgcggta tgccattcaa ccccggcatt cccgccagtt gtatggcaag
     5221 cgcctccagc cttcccaggc taccaacagg tttgagtaac ccgtcaatat gccgttgtgc
     5281 acgcgacata gcggcaggat cgatagcagg gatcgtattc agtaaatcgg caagtgtttg
     5341 catgtcacgt ctctttatag cagtgccagc aggaagacca gttcaccaag ctctatagca
     5401 gctcccagcg tatcgccggt ttgcccgccc agcgtacgtt tgagaagctg accgaggatg
     5461 aaaatcgcca ccatcgtcac caccatagca gccacaccat gcatacccgg caataatacg
     5521 gcagcaaaaa tagcggctaa gccgagtgtg acgcaggttt gtcgcccatc aattttgcca
     5581 atgaacacat tgccaagccc ttcttcccgg gcataacgat gacgatacat caataatgcg
     5641 gcagtcccgc ggctgaccgc acatgctgcc gccagcgatg caaggatcgg ttcgccacgc
     5701 agagccaact cgcttaatac cagaatcttt gccagtacaa caaaaatcaa tgccagaccg
     5761 ccatgggtgc ctaaacggct atcacgcatg atttccaaca tgcgatcgcg gctacgtgca
     5821 gaaaatacgc catcacaggt atcggcgagg ccgtctaggt ggaatccccc ggtcatcagc
     5881 gccagcacaa gtacgctaaa cagtgccgcc agtggtacgc cacaccatgc ctgcagcacc
     5941 atgaagacca gcccgctaat cgcgccaagc aataatccaa ttaaaggaaa agtaataata
     6001 ccgcgagaat aatgctcgaa atccagtccc tgggacctgc gacgcggtac aggcagacgc
     6061 gtaataaatg agagcatcgc ccaaaataat ttactcattt gatttttact ccaatacccg
     6121 aaaccaccag ccatacttca tttgccgcag cggccaactg ctgatttacc cgcccggcaa
     6181 tatcacgaaa atgtcgtgcc agacgactct ccggcacaat ccccattccc acttcgttag
     6241 tcactaatac aacctttgcg gggcaacgtt ggcaggcagc aatcaacgac tgaatctcag
     6301 cattaatcgc ctgttccatc gcctgataat cccattcatc agggtcttta tcgccgccat
     6361 aatcaaacaa cagattagtc accattgtgg taacgcattc aagcaacaca acctcattcg
     6421 ggttaatgtc tgcatgaatt aattcatcaa gatgttgcca gcgctccact gtgcgccagt
     6481 gctccgggcg gccttgccga tgatgttcta tccgtgcagc catctcatca tcaaggattt
     6541 gcgaggtagc gatatacaga acctgtgaag agtccccaat aagcgcctct gcgtggcgac
     6601 tcttcccgct ccgtgcgccc cccgtcacca aaatcatcat accgactcct gattcaaatg
     6661 tatgatggta ttcattgaac agaacaatgg atcccccacc gttgctgacc gcaaaacatt
     6721 tacgcgatta atactgcgcg taatataatt aaaaatccag cattctcaat ccattacgaa
     6781 ataagatggc attacggaat agccagactt tctcgtgtta acgtccgaca aaatgcgtca
     6841 ttgacagcgg tttctgtgga ttgacacatt tcattcagat aatgaattaa tgctactgcc
     6901 ggaacaacca gcaaacgggc attgtgttct gaaatccagg ctattgattc aacgtcagcg
     6961 acagatctgc gctgtaaaag aacttgtaaa acaacgtaat agaattatcc ggaatggtgg
     7021 cgactatgca ctagaacacc acaatttcgc tctctcggta aggcactgtc acagcattca
     7081 acagaatgtg acttgccaga tttattagcg ccaccagatg tttaaccggg taaccacgac
     7141 ccagtcgggg atatattcca tcttctcgct gacatcatca agaataaaaa ggtttatcac
     7201 actcagagca acgttcttca gcaggcggga taattcttca acatttagaa gatgtgttat
     7261 taaaggcata caaactggac gtattgtatt ttcttttggt ggttgaccta aggtagcagt
     7321 ttatcctgat gcgctgagat ttctgtaata tctacgtcaa cattctcctc gaatagaaaa
     7381 tgcagccccg gcaagtgatt cacatttttt aacaaaacgt tgttagcgtg ccattttcag
     7441 aagctctcca agaaagcgaa ttcttttctc aaattctgca ttctcagaca cagctttcta
     7501 ctccatggta atggcccaaa tacgaagttg ctcaggatca ttagtggtgc tgagaagtta
     7561 aataaccatg cggagtacca ctcgtcatca cgtatttata tatcatgaag ctattgttgt
     7621 gttttgtaca tcagtagata tattctgata tactcctttt gctagacata acctttcacc
     7681 tgcttgcaaa gcttctgtgt tctgacattg ccaaattgtt gcaattctgt atccagcctt
     7741 ctttcagtca tagcttcggg ccgcgataag actcactgat ctgaccctga ttcctcttgc
     7801 agactttata gaccaattaa aatgcagttt ctgcaggtca acgtctgacc atcattgtca
     7861 tcactctggc cattagagta accttctgca ttcatccttt tgtaaaaagt ttatgtcagt
     7921 atcagcaatt aaccggacct gatactgata tgagtcttac cgcatatacg gtcaatttca
     7981 gcaattaatt acattatcca cgccaaagtg tttgtcatcg caatgatggt accttctttc
     8041 agacaccatt ttttcaactc cgttttccac ggaccgcact cttatgtcaa gagtgcggtg
     8101 atcctaccca cgtaatatgg acacaggcct aagcgaggtt ctggttttca aattgttccg
     8161 gactgaggcc gccacaccaa ctgtgccgcc gccaccgatt gtaatcacat tcgatataat
     8221 taaacaccgt tgcccgcatt atttcccggc tgataaagtg ttctccatgg atacattcca
     8281 ctttcagcga atgaaagaag ctttccacgc agattatgcc gcttcagttg cgcctgataa
     8341 tctgctgaac agtactggcc tccacggtcc gtgtgaacga taacgttccg gggcctctta
     8401 cgccgcca
//
LOCUS       DAZGJW010000181         8391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZGJW010000181 DAZGJW010000000
VERSION     DAZGJW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.181"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            105..533
                     /gene="uspC"
                     /locus_tag="ACHKIK_004403"
     CDS             105..533
                     /gene="uspC"
                     /locus_tag="ACHKIK_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO9085447.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(540..1964)
                     /gene="otsA"
                     /locus_tag="ACHKIK_004404"
     CDS             complement(540..1964)
                     /gene="otsA"
                     /locus_tag="ACHKIK_004404"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO9085448.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(1939..2739)
                     /gene="otsB"
                     /locus_tag="ACHKIK_004405"
     CDS             complement(1939..2739)
                     /gene="otsB"
                     /locus_tag="ACHKIK_004405"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO9085449.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2906..3892)
                     /gene="araH"
                     /locus_tag="ACHKIK_004406"
     CDS             complement(2906..3892)
                     /gene="araH"
                     /locus_tag="ACHKIK_004406"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO9085450.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(3907..5421)
                     /gene="araG"
                     /locus_tag="ACHKIK_004407"
     CDS             complement(3907..5421)
                     /gene="araG"
                     /locus_tag="ACHKIK_004407"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO9085451.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5491..6480)
                     /gene="araF"
                     /locus_tag="ACHKIK_004408"
     CDS             complement(5491..6480)
                     /gene="araF"
                     /locus_tag="ACHKIK_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO9085452.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7277..7780
                     /gene="ftnB"
                     /locus_tag="ACHKIK_004409"
     CDS             7277..7780
                     /gene="ftnB"
                     /locus_tag="ACHKIK_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO9085453.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(7859..8110)
                     /gene="yecJ"
                     /locus_tag="ACHKIK_004410"
     CDS             complement(7859..8110)
                     /gene="yecJ"
                     /locus_tag="ACHKIK_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO9085454.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMQAIQKAIALIELKFTPQGESH"
     gene            complement(8225..8311)
                     /gene="azuC"
                     /locus_tag="ACHKIK_004411"
     CDS             complement(8225..8311)
                     /gene="azuC"
                     /locus_tag="ACHKIK_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO9085455.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
BASE COUNT         2174 a         2130 c         1926 g         2161 t
ORIGIN      
        1 atcagactaa tatttacctg tttgaccgag ttgggattgc gtcgtttctc cattaggagt
       61 aaagctttaa tgtcacctga agttcacaga ataaggaaca ggttatgagc tatagcaata
      121 ttcttgtcgc tgttgcggtt acaccggaaa gtcagcaact gctggcaaaa gcggtatcta
      181 tcgccaggcc agtaaaggga cacatcagtt taattactct cgcttccgac ccggaaatgt
      241 acaatcaatt agccgcgccg atgcttgaag atttacgcag cgtgatgcaa gaagaaacgc
      301 aaagctttct tgataagtta attcaggatg cggggtatcc cgttgacaag acgtttattg
      361 cctacggtga gttaagcgaa catattctgg aagtatgtca caaacatcat ttcgatttgg
      421 tgatttgcgg taatcacaat cacagtttct tttcgcgagc atcctgctcg gcgaaaagag
      481 ttattgcctc aagtgaggtc gatgtgctgt tagttccact tacgggagat taaccgctcc
      541 tacgcaagct ttggaaaggt agcaacttta tcgcgctgct ggctttccgc gcttcgcgga
      601 actatctgct ttaggtcgct aatgaagcac tcctgccagt ggttaatatc gtttttcacg
      661 ataacgtcca gcatttctgc atgacgggaa atacgttccg ccagcgacat agtcaatgca
      721 cgatccagcg cagctgcaac ttcgtcacga tcgtaggggt taacaattaa cgccgacgtt
      781 aactcgtttg ccgctcccgc aaattgcgaa agaacaagaa cgcccgggtt ggctgggtcc
      841 tgagcagcaa catactcttt tgctaccagg ttcatcccgt cacgcagtgg cgtcactaag
      901 cccacgtcag agtagcggaa tattttcatc agtaatttac ggtcaaaatg ctgattcaaa
      961 taataaagcg gcgtccagcc taattgcccg tatttaccat taattcgtcc agcttcattt
     1021 tcgagctgat gacgaatatc ctgataggct tgcacatcac cacgcgacgt tggtgcaatc
     1081 tgggtataac gaattttacc atgatgctgc ggatattttt ccagcaacgc ttcataggcg
     1141 agaaaacgct ctggcaaacc tttggaataa tccagccgtt cgacagaaaa gatattttgt
     1201 acgtttttca gttccgcttt aagttgcgcc agttttggcg gcagtggccc ggcagcctgt
     1261 ttggctattt ctttcggttc aatgccgatc gggtagactt ctgttcgaaa tgctttgccc
     1321 caggctgtat ggctttttgc gctacgtgtc gtgacgcggg tcaggttaga aagacaatcc
     1381 aggaacgcca gacgatcgtt ttctgtctgg aaacccagca aatcataatc acaaagctgt
     1441 tcaagcaagg tgtcatatgt cggcagcgcg ttgaagattt ccggtgtcgg gaaaggaata
     1501 tgcagaaaga aaccaatgcg attattcact ccccgtttgc gtaattcatg cgcaaatggc
     1561 aacaggtgat aatcgtggat ccagataatg tcatcgtctt gcaacagcgg cagtaattta
     1621 tctgccagca acgcatttac gcgtagatag ccgtcccagg caggacgctg aaattgcacc
     1681 agatcgagcc gataatgaaa agcgggccag agaacggcat tggagaattg gttgtagtat
     1741 tcgtcaaggt cctgttcgct gaggttaaaa gaggcccacg taatgttacc ttttttcacc
     1801 ttttttagcg gctgatcctc attccctgtt tcaccactcc agccaaacca cagtccgcct
     1861 gcggctttca gtgcccccag tatgccaacg gcaaggccac cggcactggc ggcgtgctcg
     1921 tctggtggtg caatccggtt agatactacg actaaacgac tcatagtcat cactcctgtt
     1981 attttctctt ttttgttgta atgcggtggt tatcatttca agccagctcc agacatccgg
     2041 cacacccgcc agtcgccatg atgcctgagt tgcacctgtg ccaattttta ctgacattcc
     2101 gcccagtcgg ttaacgactg cgaagccaga ttcatcggtt aaatcatcgc ccagaaatac
     2161 gggcgttcgc ccgataaagg gagcttcctg cataaaagct gcaattgcct cacctttact
     2221 ggtacctctc ggtttgatct cgacaacaca ctttccctgc tgtaacgcca tttgtggcca
     2281 gatctgagta atacgttgcg ctaatgtcat taatgcgtct tcatgctgcg gagcctgacg
     2341 ataatgcagc gcaaaagcca tccctttcgc ctccagctcc gcgccgggat actgagcgat
     2401 gactgtatgc agttgcacgc taatatcacg cgcaatcgca tccggcagat gaacgatatg
     2461 tgttttacca ttgatgtcac ggcgctccgc cccatgcacg cccgctaacg ggaagcgata
     2521 aggttttgcc agtgcgtcaa gctccaccat tgagcgccct gatatcaatg ccaatgcacc
     2581 atcacttgcg gttgccagta gctgtagtcc ttgcagaata ttgtcaggca cgacgacctg
     2641 atcgggatgc ggtttgattt ccgccagcgt tccatcaaga tcaaaaaacc aggcatattt
     2701 cgcggatagt tcaggggttt cggttaacgg ttctgtcacc cggctctcct ccttctcttt
     2761 tcttattgtt ttgcgcagaa cgcgctttac agacatgtaa gtatagacag tgtgacgggg
     2821 gtcgccattt gcaatgaaga acagccaacc gttgtggcgg ctggctgtgg tgggaaaaaa
     2881 cgttaaattg ttgtggaaaa aagcatcaga cagtgcgttt cgctttttgc ttgtaacggt
     2941 cgaagatcac cgctgccagc aggattaagc cgcgaaccac gtactgcgcg aaaggagaaa
     3001 tattaagcag gttcatggcg ttttccacgg tgcctaaaat taagataccc gccaccacat
     3061 atgagatttt tccgatgcca cctttcagag aaacgccacc taaaacgcag gcggagataa
     3121 caataagctc ataaccaatc gacgtcattg gctgcccact agtcatacgt gaagccagaa
     3181 taattccggc tatcgcagat accaggccag agagaacaaa gataataatt ttggtgcgaa
     3241 caaccggtac acccgccaga cgcgcagcct cttcgttccc gccaatcgcc agggtgttac
     3301 gaccaaaggt ggttttattc agcaacaaac caaagataat cagacacgcg acggtgagcc
     3361 agattggcgc aggcagaccg aaccagttag cgtaaccaag ggcaaagaag ctttcatctt
     3421 cgataccgac cgctttaccg tctgaaatga tgtacgccag acctcgaaca atctgcatcg
     3481 ttgccagtgt tgtgatcaga gcatttattt tcagtttggc gataacaaag ccattgacca
     3541 ggccacagag aatgccaagc agcaaccccg ctgccacgcc aatccacagg ctttcagtca
     3601 ggttgataac caccgccgtg gtgacacccg cacaggcaat tacggaggcg acagaaaggt
     3661 caaagtcacc ggaagcaagg cagaacaaca tgccacacgc caccatcccc gacatggaaa
     3721 ttgccaggcc caaccctttc atattaatga aggtggcaaa atttgggaca aaaatggcac
     3781 aggcgataaa gagcaccgca aacaccacca gcatgccgta ctgatcccag atacgcccga
     3841 agctgaatga cgacttaggt gcgccagacc ccgatgtaga aacagaagac atcatactct
     3901 ccttactcag gcaactgcct ggctgacttt aggcatcgca aggctcagtg cctgacgctc
     3961 atctgcctgc tcgtgtaaca attcaccggc gatttcacct tcccgcatca ccacaatccg
     4021 gtcggcaacg ccgaggactt caggtaagtc gctggaggca aacagcaccg ccacaccctg
     4081 cgccgccagc gcataaatca cgttgtaaat ttcatgctta gcaccaacat caatgccgcg
     4141 cgtaggttca tccagcaaaa tgaccttcat ctcttccgat aaccagcggc ccagaatggc
     4201 tttttgctga tttccgcctg agagattcat gatcagttgc tcagcgcccg gcgttttgat
     4261 gttgagcgaa cgaatgtggt gatcggcatt gttttcttcc caaccgttgt tgattacaca
     4321 accgccgagc acatgtttac gtctggcact gatgttgata ttgtcgcgaa cggagtgcac
     4381 gggaataatg ccttccgctt tgcgatcttc cgggcagagc atcatgcctg cggcaatggc
     4441 gtggctcggt ttacgaatat cgatcggctg ttggtcgata taaacctgac cggcggtgat
     4501 ttgcgtcccg ccaaacaagc ctttcattaa ttcgctacgc cccgccccta ccagaccaaa
     4561 cagcccaaca atttcaccac tgcgaaccgc cagacttatt ggcgtacgca cgcctggtgc
     4621 tttcacagca tcaagacgca ggcgctcctc gccataacta cgaggttgcc agccgtagat
     4681 atcgccaatg tcgcgcccga ccatcgcctg caccagcgcg tcgtggtcaa cctgctgcat
     4741 atcggtaaag gttttgacat aacgtccatc tttaaagaca gtgatggcat cgctgagggc
     4801 aaatatttct tccatacggt gagaaacgta taagatcacc cgcccctctt ttcgcagttc
     4861 acgaataacg cggaaaagat tgtcgatttc gcgggcagaa agggagctgg ttggctcatc
     4921 aaaggcgata attttagcgt tacgcgccag cgccttggcg atttcaacca tctgccactg
     4981 accaatggag agatatttca gcggcgtgtc cgggtcaata tccataccaa gatgtttaag
     5041 ttgtaaaccc gcctcataat tcagcaatga gcgattcaca atgccgcctt tatgcggcag
     5101 ctggccgaga tagatgtttt ccgcgacggt catttccggc acgagatgta gttcctggta
     5161 aataatcgcc acacccgcgt taagtgctgc ggtcgtgtcg gaaaaggaca tttcctgccc
     5221 attaatcact acagaacccg tggttggcgc atagttgccg ctgaggattt ttaagagagt
     5281 tgattttcct gcgccatttt cacccatcaa cgcatgaacc tgaccggcat agcagtcaaa
     5341 actaatatcc gtcagcgcct taacgccggg aaacgtttta ccgatgccgc gaaatgagag
     5401 atacggggta gactgttgca taacgtctcc gtgaatcact ggtcgttcac accccctctg
     5461 catcatgcag aggggaattt ttccggcaaa ttacttaccg cctaaacctt ttttctccag
     5521 ttcttcttta aagttgtcac gcgtgatcag taccacgtcg gtaacttcgg taaattttgg
     5581 cggttcaacg tcttttgcta cccagttgta aagcatttcg ctggatttat agccatgtac
     5641 gtccgggctt ggcagcaggg aaccgtagaa gccggttgcc tgtgctttag acagttcgct
     5701 caccgcatcc acaccgttaa tgccaatgcc gatgatatcg gccgctttaa agccctgacc
     5761 ttccgtcgcg cgtacgccgc ccagcacggt gctgtcgttc ataccgacga tcagccaatg
     5821 tttaacttcc ggatgttgaa ccagcattga gttggcagcg tcaaatgccc ccgggatgtc
     5881 gttagatttg gtaggtacct gataaatttg tttttccggg aatccggccg ctttcagcgc
     5941 atccatagat cccgtagtac ggcggcgggc ggtatccagt tcgttggcgg taatcgccat
     6001 caccgcgctt tctttgacat cccagccacg tttctgcatc tctttataca gttcctggcc
     6061 ctgacgttcg ccaattttag tcgccgccat catcaccagc ggaacggtat ccattggctt
     6121 acctttggcg ttaacaaact ggtcatccac ggcaatgact ttcatatcgt agccacgcgc
     6181 tttcgcgacg atggcagagc cgagtttggg gtccggagta caaataacga aaccttttgc
     6241 gccactggca gccaggctgt cgatcgcgtt caatgttttt tcgccatccg gcacggcaat
     6301 cttaataacc tcaaacccta aatccttccc ggctttatcg gcaaacttcc attcggtctg
     6361 gaaccacggc tcttccggtt gcttcaccag aaaaccgagc ttcaggttct ccgccatagc
     6421 ggattgtgac ataacggctg ccagaccaat ggctgccagg gctttagtaa atttgtgcat
     6481 ggttctctcc agctttagtg tcgttttgtg tagggcaaaa acgaatgaca ttcgttaaat
     6541 taatcggaaa acaaagcatt accttttaac taaaagataa gtgactgtgt tgacatagtt
     6601 ttagcgagaa attaattctc cataggagag caatatcaca tcgcagaatt acagtgagaa
     6661 cgtgcataaa tttagcggga aaagacataa gggaaagcca atttgtcaga caaattgtcg
     6721 aatgcacagc agattaatcc ataagattag cctggaaatc cttgttgtct ttggtaccca
     6781 tgcgggatgt cttcttttta accagtcaat aggccgcatt acctggcgtt gagtttttga
     6841 aatggtgtaa taaccgcaac tcaaagatgt ggaaaatgca cgtcattcat ttcgccatta
     6901 attatcactg tactcattaa ttaacagaac acgtataatg agagccatct cgcaaaaatg
     6961 aaaaaacgtt ttataaaatc atcacttcat catgaattca aattcattga ttaatatcaa
     7021 caagatacaa aaagcgctat cattaaaatt cattgcagtt acattgattt catcaatgaa
     7081 atgtaaaaat atataaactt gatgatttaa gcattttctt atacccgttc agacgttatt
     7141 cttatttcag atcatcgtca gaattgactc cacgatcaca tttcggaccg gcagaaagga
     7201 attattctgc aaacagtaat tatggtgttt tgatttatct tgcacctctc cacttctgga
     7261 tataaggata ttaggtatgg caaccgctgg aatgcttctc aaactcaact ctcaaatgaa
     7321 ccgcgagttt tacgcatcca atctctacct tcacctgagt aactggtgtt ctgaacagag
     7381 tctgaacggc accgccactt tccttcgcgc ccaggcacag agtaatgtga cccaaatgat
     7441 gcgcatgttt aactttatga agagtgtcgg cgctaccccc atcgttaaag ccattgatgt
     7501 tcccggtgaa aaactgaact ctctggaaga actgttccaa aaaacgatgg aagaatacga
     7561 gcaacgttct agtacgttgg cacagttagc cgatgaagcg aaagaactga atgatgattc
     7621 aaccgtcaat ttcctgcgcg atctggaaaa agaacagcag catgatggtc tgttgctgca
     7681 aaccattctt gatgaagtgc gcagtgcgaa acttgcgggt atgtgccctg tgcagaccga
     7741 ccaacatgtt ctgaatgtcg tgtcacacca gctgcattga tcatcatcgg cgctaatgca
     7801 ttgcgccgat gaaggttttg agaaaccgca gcctcatctg tttgaagcag cggttttttt
     7861 aatgggattc accctgtggg gtaaacttga gttcaataag cgcgatggct ttttggattg
     7921 cctgcatggt gaccgggtct gcggcggcgg gatggttagt aaagtcgata ttcttcagct
     7981 gactggacat tttttcacga acttcaacgg gcgcgattac atcgagaaca tccagaattt
     8041 gtttgataac caactggcaa gcaaccacat cagaaaccag ttcctgatcg gcattcagcg
     8101 gctgggacat cgtaaactcc tgatagcatt ttgaaagccg ttatagtagc ggcttcacat
     8161 cttcagcgat agtcacatcc accgtcatca ggacacaaaa aaacctgccg gagcaggttt
     8221 tttattatcg gaacatattg cctggcggta cgtctttgaa cgtcttgcaa tagttattga
     8281 acatactttt caggattttg cgcagtttca tcgcggcact ccgaccattt gttatacagg
     8341 tgttattctc tttgcgctta taatatgaca accatcacaa aaatcaatct t
//
LOCUS       DAZGJW010000182         8324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZGJW010000182 DAZGJW010000000
VERSION     DAZGJW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.182"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1261
                     /locus_tag="ACHKIK_004412"
     CDS             <1..1261
                     /locus_tag="ACHKIK_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFO9085456.1"
                     /translation="DKFNSDLYTLALLNTKVFNVLLKALNPTINTQVNDVMSLPFIKM
                     EAAVQEKIETNEQLLINIAMHDWNSQEISFDFMVNSLVSESRASSEVASSYAIARKKW
                     QDAFNKTKELEEENNHLVITSSNFNGINEELIDSEITLVSNPYYKYGDSTTNNETRLL
                     SDSIADVLSYSIGCMMGRYSLDREGLVYAHEGNKGFAELAAEGAYKTFPADNDGILPL
                     MDDEWFEDDVTSRVKEFVRTVWGEEHLQENLEFIAESLCLYAIKPKKGESALETIRRY
                     LSTQFWKDHMKMYKKRPIYWLFSSGKEKAFECLVYLHRYNDATLSRMRTEYVVPLLAR
                     YQANIDRLNDQLDEASGGEATRLKRERDSLIKKFSELRSYDDRLRHYADMRISIDLDD
                     GVKVNYGKFGDLLADVKAITGNAPEAI"
     gene            1438..4035
                     /gene="pglZ"
                     /locus_tag="ACHKIK_004413"
     CDS             1438..4035
                     /gene="pglZ"
                     /locus_tag="ACHKIK_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFO9085457.1"
                     /translation="MQNQDFIAGLKAKFAEHRIVFWHDPDKRFIEELEQLKLENVTLI
                     NMTHASQLAVKKRIEMDEPEQQFLLWFPHDAPPHEQDWLLDIRLYSSEFHADFAAITL
                     NTLGIPQLGLREHIQRRKAFFSTKRTQALKNLVTEQEDETSLDKKMIAVIAGAKTAKT
                     EDILFNLITQYVNQQTEDDSELENTQAMLKRHGLDPVLWEMLNHEMGYQAEEPSLENL
                     LLKLFCTDLSAQADRQQRAWLEKNVLLTPSGRASALAFMVTWRADRRYKEAYDYCAQQ
                     MQAALRPEDHYRLSSPYDLHECETTLSIEQTIIHALVTQLLEESTTLDREAFKKLLSE
                     RQSKYWCQTQPEYYAIYDALRQAERLLNLRNRHIDGFHYQDSATFWKAYCEELFRFDQ
                     AYRLFNEYALLVHSKGAMILKSLDDYIEALYSNWYLAELSRNWNEVLEAENRMQAWQI
                     PGVPRQQNFFNEMVKPQFQNPQIKRVFVIISDALRYEVAEELGNQINTEKRFTAELRS
                     QLGVLPSYTQLGMAALLPHEQLCYQPGNGDIVYADGLSTSGIPNRDTILKNYKGMAIK
                     SKDLLELKNQEGRDLIRDYEVVYIWHNTIDATGDTASTEDKTFEACRTAVTELKDLVT
                     KVINRLHGTRIFVTADHGFLFQQQALSVQDKTTLQIKPENTIKNHKRFIIGHQLPADD
                     FCWKGKVADTAGVSDNSEFLIPKGIQRFHFSGGAHFVHGGTMLQEVCVPVLQIKALQK
                     TAAEKQPQRRPVDIVAYHPMIKLVNNIDKVSLLQTHPVGELYEPRILNIYIVDNANNV
                     VSGKERISFDSDNSTMEKRVREVTLKLIGASFNRRNEYWLILEDAQTETGYQKYPVII
                     DLAFQDDFF"
     gene            4046..6130
                     /gene="brxL"
                     /locus_tag="ACHKIK_004414"
     CDS             4046..6130
                     /gene="brxL"
                     /locus_tag="ACHKIK_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFO9085458.1"
                     /translation="MQTHHDLPVSGVSAGEIASEGYDLDALLNQHFAGRVVRKDLTKQ
                     LKEGANVPVYVLEYLLGMYCASDDDDVVEQGLQNVKRILADNYVRPDEAEKVKSLIRE
                     RGSYKIIDKVSVKLNQKKDVYEAQLSNLGIKDALVPSQMVKDNEKLLTGGIWCMITVN
                     YFFEEGQKTSPFSLMTLKPIQMPNMDMEEVFDARKHFNRDQWIDVLLRSVGMEPANIE
                     QRTKWHLITRMIPFVENNYNVCELGPRGTGKSHVYKECSPNSLLVSGGQTTVANLFYN
                     MASRQIGLVGMWDVVAFDEVAGITFKDKDGVQIMKDYMASGSFSRGRDSIEGKASMVF
                     VGNINQSVETLVKTSHLLAPFPTAMIDTAFFDRFHAYIPGWEIPKMRPEFFTNRYGLI
                     TDYLAEYMREMRKRSFSDAIDKFFKLGNNLNQRDVIAVRRTVSGLLKLMHPDGAYSKE
                     DVRVCLTYAMEVRRRVKEQLKKLGGLEFFDVNFSYIDNETLEEFFVSVPEQGGSELIP
                     AGMPKPGVVHLVTQAESGMTGLYRFETQMTAGNGKHSVSGLGSNTSAKEAIRVGFDYF
                     KGNLNRVSAAAKFSDHEYHLHVVELHNTGPSTATSLAALIALCSILLAKPVQEQMVVL
                     GSMTLGGVINPVQDLAASLQLAFDSGAKRVLLPMSSAMDIPTVPAELFTKFQVSFYSD
                     PVDAVYKALGVN"
     gene            complement(6395..6820)
                     /locus_tag="ACHKIK_004415"
     CDS             complement(6395..6820)
                     /locus_tag="ACHKIK_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO9085459.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCGQHF
                     PGKKFCEACGTGIYSRAVCTKYVYSYQPDLFQDCGSAST"
     gene            complement(6817..7200)
                     /locus_tag="ACHKIK_004416"
     CDS             complement(6817..7200)
                     /locus_tag="ACHKIK_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO9085460.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
     gene            complement(7572..8171)
                     /locus_tag="ACHKIK_004417"
     CDS             complement(7572..8171)
                     /locus_tag="ACHKIK_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9085461.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
BASE COUNT         2192 a         2052 c         2095 g         1985 t
ORIGIN      
        1 cgataaattt aacagtgatc tttatacgct tgctctcttg aatacaaaag tttttaatgt
       61 tctacttaaa gcattgaacc ctactattaa tacccaagtt aatgatgtca tgtcattacc
      121 attcatcaaa atggaagcag ctgttcaaga aaaaatcgaa actaatgaac agctgctgat
      181 aaatatagcg atgcacgatt ggaattcgca agaaatttct tttgatttta tggttaattc
      241 tttagtcagc gaatcgagag catcatctga agtagcatca agctatgcta tagcaagaaa
      301 aaagtggcaa gatgctttta ataaaacgaa agagttggaa gaagaaaata accatttagt
      361 cattacaagt agcaacttta atgggataaa tgaagaatta attgattcag aaataacttt
      421 agtcagcaac ccatactaca agtacggtga ttcaacgact aataatgaaa ctcgtttgtt
      481 atcggattca atagcagatg tattaagtta ttccatcggc tgcatgatgg gtcgctactc
      541 cctcgatcgc gaaggcctcg tctacgccca tgaaggcaac aaaggctttg ccgaacttgc
      601 cgctgaaggt gcgtacaaaa ccttcccggc agacaatgac ggcatcctgc cgctgatgga
      661 tgacgagtgg tttgaggacg acgtcacctc tcgcgtcaaa gagtttgtcc gcaccgtctg
      721 gggcgaagag cacctgcagg aaaacctcga atttatcgcc gaaagtctct gtttatacgc
      781 gatcaagccg aaaaaaggcg aatctgcgct ggagaccatt cgtcgctatc tttccacaca
      841 gttctggaaa gatcatatga agatgtataa aaagcgccca atctactggc tattcagctc
      901 cggtaaagag aaagccttcg agtgcctggt gtatctgcat cgctataacg acgccacgct
      961 gtcgagaatg cgtaccgaat atgtggtgcc gctgctggca cgttatcagg ccaatatcga
     1021 tcgcctgaac gatcaacttg atgaagcttc tggcggtgaa gccacacgcc tgaaacgcga
     1081 acgcgacagc ctgatcaaaa aatttagcga actgcgtagc tatgacgacc gcctgcgtca
     1141 ctatgctgat atgagaatca gtattgatct cgacgatggc gttaaggtta actacggcaa
     1201 gtttggcgat ctgctggcag atgtcaaagc catcaccggc aatgccccag aggcgatcta
     1261 aaccagacgg cgcgttcccc tactgccggg ttctgcccgg tggcaaatac caccgggaaa
     1321 cgcgccgctg ctggcatttc tccacctcac ttcatgataa aatgcgccac cgtgtcaaaa
     1381 tctccttttc gcgttttggc gctttcttat tcatcgtaac aacatgggat tgtgaacttg
     1441 caaaatcagg actttattgc tggccttaaa gctaaatttg ccgaacatcg catcgttttc
     1501 tggcacgatc ccgataaacg ttttattgag gaactggaac agctcaagct tgaaaacgtc
     1561 acgctaatca acatgaccca cgcgtcacag ctggcggtga aaaaacgcat cgagatggat
     1621 gagccagaac agcagttcct gctgtggttc ccccatgatg cgccgcctca tgaacaagac
     1681 tggctgctgg atatccgcct ttacagcagc gaattccatg ccgattttgc cgccatcacc
     1741 ctgaacacgc tgggcattcc ccagcttggc ctgcgcgagc atattcagcg acgcaaggcc
     1801 ttcttcagca ctaaacgtac gcaggcgctg aaaaacctgg tgacggaaca ggaagatgaa
     1861 acctcgctgg ataagaaaat gattgcggtg atcgctggcg caaagaccgc gaaaaccgaa
     1921 gacattttgt ttaacctgat tacccagtac gttaaccaac aaacagaaga cgacagcgaa
     1981 ctggaaaaca cgcaggcgat gttgaaacgc cacggtctgg acccggtatt gtgggaaatg
     2041 ctcaaccacg aaatgggcta ccaggcagag gagccatcgc tggaaaacct gctcctgaaa
     2101 ctgttttgta ccgatctctc tgcccaggcc gacagacagc agcgcgcctg gctggaaaaa
     2161 aatgtcctgc tgacgccatc cggcagagca tccgccctgg catttatggt gacctggcgt
     2221 gccgatcgtc gctataaaga ggcttatgac tactgcgctc agcaaatgca ggcggccctg
     2281 cgcccggaag atcattaccg actcagttcg ccgtatgatt tgcacgaatg cgaaaccacc
     2341 ctcagcatcg aacaaaccat tattcatgcg ctggtaacgc aactgctgga agagagcacc
     2401 acgctcgatc gggaagcctt taaaaaactg ctctctgaac gccagagcaa atactggtgc
     2461 cagacacaac cagagtatta cgccatctat gacgcgctgc gccaggctga gcgtttgctg
     2521 aatctgcgca atcgccacat cgacggtttc cactatcagg acagcgccac cttctggaaa
     2581 gcctactgcg aagaactgtt ccgcttcgac caggcttatc gcctgtttaa tgaatatgcc
     2641 ctgctggttc acagcaaagg ggcgatgatc ctcaagagcc tggatgatta tatcgaggca
     2701 ctctacagca actggtatct ggcagaatta agccgtaact ggaatgaagt gctggaagcg
     2761 gaaaatcgta tgcaggcgtg gcaaattcct ggcgtgccgc gtcagcagaa cttcttcaat
     2821 gagatggtga agccgcagtt ccaaaatccg caaatcaaac gcgtgttcgt gataatttcc
     2881 gatgccctgc gttatgaagt ggcggaggag ctggggaatc aaatcaatac cgagaaacgc
     2941 tttaccgcag aactgcgatc gcagctcggc gtgctcccta gctacaccca actgggaatg
     3001 gcggcgttgc tgccccatga acaactttgc tatcaacccg gtaacggcga catcgtttat
     3061 gctgatgggc tgtcgacctc gggtattcct aaccgcgata ccattctgaa gaactataag
     3121 ggaatggcga taaaatcgaa ggaccttctg gagttaaaaa accaggaagg gcgagacctt
     3181 attcgcgatt acgaagtagt gtatatctgg cataatacga ttgatgccac tggcgacacg
     3241 gcatccacgg aagataaaac cttcgaagcg tgccgcacgg cggtgactga actgaaagat
     3301 ttagtcacca aggtaatcaa ccgtctccac ggcacacgca tttttgttac ggcggatcac
     3361 ggtttcctgt tccagcaaca ggcgctttcg gttcaggata aaaccactct gcaaattaag
     3421 ccggaaaaca ccatcaagaa ccacaaacgc tttattatcg gccaccagct tcccgccgat
     3481 gatttttgct ggaaagggaa agtggcggat accgcaggcg tgagcgacaa cagcgagttc
     3541 cttattccga aagggatcca gcgcttccat ttctctggcg gcgcgcactt cgttcatggc
     3601 ggcaccatgt tgcaggaggt ttgcgttccg gtattgcaga taaaagccct gcaaaaaacc
     3661 gccgcagaaa aacagccgca gcgccgcccg gtggatattg tcgcttacca tccgatgatt
     3721 aagctggtga acaatatcga taaagtgagc ctgttgcaga cgcatccggt gggcgaactt
     3781 tatgaaccgc gtatcctgaa catttacatt gtcgacaacg ccaacaatgt ggtctcgggc
     3841 aaagagcgca tcagctttga cagcgataac agcaccatgg aaaaacgcgt acgcgaagtt
     3901 acgctgaagc tgattggcgc tagcttcaac cgtcgcaatg agtactggtt gatactggaa
     3961 gacgcacaaa cggaaacggg gtatcagaag tacccggtca ttatcgatct ggcgttccag
     4021 gatgatttct tctaagtgag gcgatatgca aacccatcat gatttacctg tttcaggcgt
     4081 atccgcaggg gaaattgcct ccgagggtta cgatctggac gccctgctga accagcattt
     4141 tgctggtcgc gtggtgcgta aagatctcac caagcaactc aaggaagggg caaacgtccc
     4201 ggtgtatgtg cttgagtatc tgctcggcat gtactgcgcc tctgacgatg acgacgtggt
     4261 cgagcaaggg ttgcaaaacg ttaagcgtat tctggctgat aactatgtgc gcccggatga
     4321 agcggagaaa gtgaagtcgc tgatccgcga gcgtggttcg tacaaaatca tcgataaagt
     4381 gtcggtgaaa ctgaaccaga aaaaagacgt ttacgaagcc cagctttcta acctcggcat
     4441 caaagacgcg ctggtgccct cgcagatggt taaagacaac gagaagctac tgacgggcgg
     4501 tatctggtgc atgattaccg tcaactattt ctttgaagaa gggcagaaga cctcaccctt
     4561 ctcattgatg acgctcaagc ctatccagat gccgaatatg gatatggaag aagtgttcga
     4621 tgcgcgtaaa cactttaacc gtgaccagtg gatcgatgtg ctgctgcgct cggtgggtat
     4681 ggagcccgcc aatattgagc aacgcaccaa atggcacctt atcacccgta tgatcccgtt
     4741 cgtggagaac aactataacg tttgcgagct ggggccgcgt ggcaccggta aaagccatgt
     4801 gtataaagag tgttctccta actccctgtt agtttccggc gggcaaacga ccgttgccaa
     4861 cttgttctac aacatggcca gtcgccagat cggcctggtt ggcatgtggg atgtggtagc
     4921 gttcgacgaa gtcgcgggga tcactttcaa agataaagac ggcgtgcaaa tcatgaaaga
     4981 ttacatggcg tcaggatctt tctctcgcgg cagagattcg attgaaggta aagcgtcgat
     5041 ggttttcgtc ggcaacatca atcaaagcgt agagactctc gttaaaacca gccatttgct
     5101 ggcaccattt ccgactgcga tgattgatac agcatttttc gaccgctttc atgcctatat
     5161 tcccggttgg gaaatcccca aaatgcgccc ggaattcttt accaaccgtt acgggctgat
     5221 tacggattat ctcgctgaat atatgcgcga aatgcgcaaa cgcagtttct ctgatgcgat
     5281 tgataaattc tttaagctgg gtaacaacct caaccagcgt gacgttattg ccgttcgacg
     5341 taccgtgtcg gggttgttaa aactcatgca tcccgatggc gcgtacagca aagaagatgt
     5401 gcgagtctgc ctgacctatg cgatggaagt tcgccgccgc gtgaaagagc aacttaaaaa
     5461 actgggcggt ctggagttct tcgatgtgaa ctttagctac atcgacaacg aaacgctgga
     5521 agagtttttt gtgagcgtac cggaacaggg cggcagcgaa cttattcctg ccggaatgcc
     5581 aaagccgggt gttgtgcatc tggtcactca ggcagaaagc ggcatgaccg ggctgtatcg
     5641 ttttgaaaca cagatgactg ccggtaatgg taagcatagt gtatcgggtc tgggttcaaa
     5701 tacctccgcg aaagaagcta tccgcgtcgg tttcgattac ttcaaaggca atttgaatcg
     5761 ggtaagcgcg gccgcgaaat tctccgatca tgaatatcac cttcatgtcg ttgaactgca
     5821 taatactggc ccaagcaccg caaccagtct tgctgcgctt atcgctttat gttcgatatt
     5881 gctggcaaaa ccggtgcagg aacagatggt ggtgttgggc agtatgacgc ttggtggggt
     5941 aattaacccg gtgcaggatc ttgccgccag tttgcagctc gccttcgaca gcggtgcaaa
     6001 acgggttctg ttgccgatgt cctcggctat ggatattcca acggttccgg cagagttatt
     6061 taccaagttt caggtgagtt tttactcaga cccggttgat gctgtttata aggcgctggg
     6121 tgtgaattaa cgtagtaact attttaatga actggagagg acaatggctc aagaacgttc
     6181 tgtaattaaa ttcatcagac cttgcctctc cattcatgaa tataaaaata cttacatctg
     6241 agtattgttg gctgtgtttg aataaaaaga gagctgttca aataagcctg tataactgtt
     6301 ctttacaaaa aatggaggag ctatcagcta attcaacact ggaagatgtt cgtcgagttg
     6361 gattgaacgc cctgcgcaga ccaggcattt cggattaagt gctcgcactt ccacagtcct
     6421 ggaacaagtc tggctggtag ctgtaaacat actttgtaca taccgctctg gaatagatcc
     6481 ccgtgccaca ggcttcacag aactttttcc cgggaaaatg ctgcccgcac atcacacaat
     6541 gccactccag cacgaccggt aatggcgata aaaacatcgc catatcctca atgtaagggt
     6601 gggacttctc cggattcagc accacacagt ccgccttctg ttgctcactc agggcatgta
     6661 aatcatgctc aaaccacgcc ccctgagcat ctgtctgcag aatcaaccgg ccatggcagg
     6721 aaaggcagaa acactgccgg atatttctgc tgagcgcaga gtcggcactg ataatgtgct
     6781 cacccggcgt gatccccagc cccgttttta taccgttcat tctgctcccc cctcctcact
     6841 gaagcgcccg gtctggcagt gtgtgcagta ctgctcccca taataatcgt ggtgacattg
     6901 cctgcagtgc cagctggctt tacgtactac gggtaaggca tccgggacga attgctgcag
     6961 acgtttaatc aaccgtattt ctctgcgttc cggcctgaca taagggcact catgaccgtg
     7021 ctccgtcagc ccttcgtcag cgtgttcaaa ccagggccgt tcagtgtcgt attgcgggtg
     7081 gtatctgagt gcactgccgc aaaggtggca ggtgtagcta tcgtaaggtg cagtctgtgc
     7141 ggtacgggca ccggtcagac gtccgttgcc atcaagtgcg agaaaagatt ttgcgtacat
     7201 agtatatgtt cctctgcgcc agacgacacg caggcaacag ccgtcccggt acgggcagcg
     7261 tggtcagggt gtgaatggcg gttcagttaa aatctggact ggagtttggg taaagcaggc
     7321 tggttgctgt tacagcactg tggttgtcac acaatggccc actgacgtaa aaaacagtca
     7381 gtaagcagag atggagattg ggggatgtgc tgtatcggtt gccggaagga atggtaaaga
     7441 atgtgtcatg gttaaatctc cgtatggttc atacggaatg accacgccgt aaaaaatgcg
     7501 cagtgccgct gaagtggccg ccggaaccga cagcaaagat tagcgccagc ccgtacttgt
     7561 cgttcagggg tttacaccag agcacatatc cccggcaggg ggctgtccag tcgtggtcga
     7621 ccccggttac ttttttcagg aagcccgctg catttcagga tgatgccaca ttctttctgt
     7681 tcccgtttgg ccaggtagct gatggcatag cgtaaatcat tcaccgcttt gtgatctgaa
     7741 tagtgaatga cgtggttaat gtttaccgga taatttttat taaaccggca ccagtggtag
     7801 taaccattac cctcggtaac ccgcctccag atatctccca tcaatctgga taccagatac
     7861 gaactcctgt ggctctggcc gttcagatag cccacaaaat gaatgtgaag ccctttacgt
     7921 tctgtatatt ccatcaccca ggcatacccg accagtttct ctcccacctc gttaatcagt
     7981 tgcagcatct ccgcgcacat ggcgtacctg caggcacatc tgtagctgag ggtgtttttt
     8041 cggtaagcaa aatccattct gaacggtaac agtttcgaat agaccccgaa taatgaatcc
     8101 agatgctggt taaagtgctc caggagctgg ggagccattt cgtaagcggg attggtgtta
     8161 taagtttgca tgtttcttcc tcgtgttctg taacacagga agaagcgttg ccgtaatttt
     8221 tgaagaagat gttctcaggt agtagggtga acgtgtttcc tccggtaacg acttccacgg
     8281 gtatcagaac caggcaaaga acagcatacg taacagccat acag
//
LOCUS       DAZGJW010000183         8308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZGJW010000183 DAZGJW010000000
VERSION     DAZGJW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.183"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(22..135)
                     /locus_tag="ACHKIK_004418"
     CDS             complement(22..135)
                     /locus_tag="ACHKIK_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFO9085462.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            738..2021
                     /gene="ydfJ"
                     /locus_tag="ACHKIK_004419"
                     /pseudo
     CDS             738..2021
                     /gene="ydfJ"
                     /locus_tag="ACHKIK_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            2110..3570
                     /locus_tag="ACHKIK_004420"
     CDS             2110..3570
                     /locus_tag="ACHKIK_004420"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO9085463.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPAIGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNLIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(3606..3809)
                     /gene="ydfZ"
                     /locus_tag="ACHKIK_004421"
     CDS             complement(3606..3809)
                     /gene="ydfZ"
                     /locus_tag="ACHKIK_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO9085464.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(3987..4673)
                     /gene="rspR"
                     /locus_tag="ACHKIK_004422"
                     /gene_synonym="ydfH"
     CDS             complement(3987..4673)
                     /gene="rspR"
                     /locus_tag="ACHKIK_004422"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO9085465.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(4762..5508)
                     /gene="ydfG"
                     /locus_tag="ACHKIK_004423"
     CDS             complement(4762..5508)
                     /gene="ydfG"
                     /locus_tag="ACHKIK_004423"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO9085466.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5645..7690
                     /gene="dcp"
                     /locus_tag="ACHKIK_004424"
     CDS             5645..7690
                     /gene="dcp"
                     /locus_tag="ACHKIK_004424"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO9085467.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHSVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFRKAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(7735..8253)
                     /locus_tag="ACHKIK_004425"
     CDS             complement(7735..8253)
                     /locus_tag="ACHKIK_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO9085468.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         2103 a         1873 c         2107 g         2225 t
ORIGIN      
        1 tctgattcag gatatgttga cttatacatc ggttttgtct gggttattgg atatgccaat
       61 ccctaatttt attagggcat gactaaaaat gctgaatatg ataaggagag aagtgattat
      121 cagtatgctg ttcatatagc ctcgaattag taatgtgtta tatatgatat agttgacaat
      181 ttttatcttg ggtgttctta aagttcgtag ataaacattg tcgtttcagg tatacaggaa
      241 tgctaacagg tggcagcaaa aatcaggcgg tttatggcgc aagctgaagc ggcaactgca
      301 aactatctta tgcagagact ctacacggat tgggtttaaa agtatacata gataacagtt
      361 tttatctgaa aaagaaaaat atcaaggtga tatagcctat atgcctttga tgtggaggaa
      421 tgaatgtgat gggagtgatg tatctgaata gttgaaaaac cgcagacaca ccttatgcaa
      481 gaacgtgctg cggttggctg gtaaattttt cgatagtgcg agtattgaat gatttccagc
      541 cgttattgat tttacgcgta aatccatgaa aaaactactt atctgttggg gagttttttg
      601 gggcatatat gggacagaaa taggccccag atagacactg agatcgaact taggatgctt
      661 ttaaaaaaat gcaactatct gaaaaaacct agaaaacgcc aaggaaacca caggatggga
      721 aaaaacacct gtgaattatg gatttccagt tatattcgct cggcgcagcg ttagtgtttc
      781 atgaaatatt ttttcctgaa tcatcaacgg caatggcgtt aattttggca atgggaacct
      841 acggtgcagg ttatgtggcg cgtattgtcg gagcatttat tttcggcaaa atgggcgaca
      901 gaatagggcg taaaaaagtg ctctttatta ccatcaccat gatggggatc tgtaccacct
      961 taattggtgt gttaccgacc tatgcacaga ttggtgtttt tgcccccatc ttgctggtga
     1021 cgctgcgtat tattcagggg ttgggtgcag gtgcggaaat ttccggtgcc ggtacgatgc
     1081 tggcggaata tgcgccaaaa ggtaagcgcg gaattatctc ctcatttgta gctatgggaa
     1141 ctaactgcgg aaccttgagc gcaacggcaa tctgggcctt tatgttcttc attctcagta
     1201 aagaggaact gcttgcgtgg ggatggcgta taccgttcct ggcgagcgtt gtcgtgatgg
     1261 tctttgctat ctggttgcgt atgaatctga aagaaagccc ggtctttgag aaggttaacg
     1321 acagcaacca acccacagca aaacctgcac ctgctggtag catgttccag agcaaatcct
     1381 tctggctggc aacagggctg cgttttggtc aggcgggtaa ctccgggtta attcagactt
     1441 tccttgcagg ctatttagtg cagacgttat tgtttaacaa agcaattcca acagatgcat
     1501 tgatgattag ttcgattctc ggctttatga ccattccgtt ccttggttgg ttatccgata
     1561 aaattggtcg ccggatcccg tatattatta tgaatacctc cgcgattgtg ctggcatggc
     1621 caatgctttc tatcattgta gataaaagct atgccccgag caccattatg gttgcactga
     1681 ttgtgattca taactgtgcg gtgctgggat tatttgctct ggaaaacatt accatggcag
     1741 aaatgttcgg ctgtaaaaac cgctttaccc ggatggctat ttctaaagaa attggtggtc
     1801 ttatcgcttc cggttttggt cctatcctgg cgggtatttt ctgcaccatg acggaatcct
     1861 ggtatccgat cgccattatg atcatggcat attcagtaat tggtttaatc tctgcgctga
     1921 aaatgccaga ggtgaaagac cgtgatttaa gtgcgctgga agacgctgcg gaagatcaac
     1981 cgcgtgttgt aagagctgcg caaccttcca gaagtctgta aatcctcaat cccttctctt
     2041 accggagagg ggatttttat tcatataaca aaacatatag cttgccatat ttatatttaa
     2101 ggaattgtta tgggaaataa tttgttatca gcaaaagcga cactccctgt ttatgatcgt
     2161 aataacctgg ccccaagaat tattcattta ggctttggtg catttcaccg tgcgcatcag
     2221 ggcgtgtatg ccgatattct tgctacagaa catttcagtg actggggata ttatgaggtc
     2281 aacttaatcg gcggcgaaca gcaaattgcc gatttacaac agcaagataa tctttatacc
     2341 gttgcggaaa tgtcggccga tgcgtggacg gctcgcgtcg ttggcgtcgt taaaaaagcc
     2401 ttgcacgtac agatggatgg cttagaaacc gtgttggctg cgatgtgtga accgcaaatc
     2461 gcgattgtct ctctgacaat caccgaaaaa gggtatttcc actctccggc gatcggacag
     2521 ttaatgctcg atcacccgat ggtcgctgcc gacgtgcaaa atccccacca gccgaaaaca
     2581 gcaacaggag tgattgttga ggcgctggcc cgccgtaaag cggcaggact tcccgcattt
     2641 accgtcatgt catgtgacaa catgccagaa aacggtcatg ttatgcgtga cgttgtcact
     2701 tcatacgcga aagctgttga tgtaaaactg gcacaatgga tcgaagataa cgtgactttc
     2761 ccatcaacaa tggtggaccg tattgtgccc gcagtgacag aggatacgct ggcgaaaatc
     2821 gaacaactta ccggtgtggg cgatcctgcg ggcgttgcct gtgaaccttt ccgccagtgg
     2881 gtaatagaag ataactttgt tgccggacgt ccagaatggg aaaaagcggg agccgaactg
     2941 gttagtgatg tgctgcctta tgaagagatg aagttgcgca tgctcaacgg cagtcattca
     3001 ttcctggcgt atttgggtta tcttgctgga tatcagcaca ttaatgactg catggaagat
     3061 gaacattatc gttatgcggc gtatggcttg atgttgcagg aacaagcgcc gacgctgaaa
     3121 gtgcagggcg ttgatttgca agattacgct aaccgattaa ttgcacgcta tagcaacccg
     3181 gcgttacgtc atcgaacctg gcagattgcg atggatggta gccagaaatt gccacagcgg
     3241 atgttggatt ctgttcgctg gcatctggcg catgacagca agttcgatct gctggcgctg
     3301 ggcgtcgcgg gttggatgcg ttatgtcggt ggtgttgatg aacagggaaa tctgatagaa
     3361 atcagtgacc cactgttacc tgttattcag aaggctgtac aaagtagtgc cgaagggaaa
     3421 gcgcgcgtcc agtcattgct ggcgattaag gcaatctttg gtgatgattt gccagacaat
     3481 agcttgttta ctgcaaaagt gacggaagcg tacttgtctt tattagcgca tggcgcgaaa
     3541 gcgaccgtgg cgaaatattc cgtgaagtaa aaaaagcaat gccgccatcg gcggcattca
     3601 cacacttagt tcatgccgag acgaatcagg tccagaggtg ccagtttctc ttcacaacct
     3661 tcaacaacta cggtttttcc gcggcggatt tgctcagcgg tcagaccttc agtatcaatc
     3721 gacaggatct tgccagtgtg accggtgccg ctaaccataa cacggctgcc agtggtgatt
     3781 gcgttacggt tacgatcgta ggtcgtcata ggtgttttct cctttctgat ttacagtcga
     3841 agcccacgta acgggcgcaa tataaatact cctgtcgagt gaaatatttt ttgtttttga
     3901 tcaagctcac atcttttttg atgcatgcat gagccgtaag gcggggggaa aagcctctca
     3961 tcccggatgg gatgagagta aatgaattac tcttcgctaa accagtcgct gttttcctgg
     4021 cggatctggc gcacggattc gctgatttcc tgcaaatgtt gcgtcattgc acgttctacc
     4081 gcatcgccat cacgtttttg cagggcagag aaaatatcaa gatgctggcg taacagcatt
     4141 tctggtggag aaacatggtc gaaactcata tagcgcacgc gatcaacggt cgctttcagg
     4201 ttctcaatgg tatcccacgc cagttgacag tcggcaatct gcgtcaggag ttgatggaag
     4261 ttgtcatcaa gttcaaaaaa atcatccagt tgcttgcgct caatggcaat gcgttgctgg
     4321 tgaagatttt gttccagttg gtagcactgg ctttcggtaa tcatgctcgc cgcccgacgc
     4381 gccaccgcgc actcaatggc ctgacggata aaactgccgt ttcgcacctg ggccatggaa
     4441 attttgttga cgtagctgcc acgctgcgga cgaatttgaa tcaggccgtt ttccgccagt
     4501 ttaataaagg cttcacgaac cggctggcgt gacacattga aacgaacaga aacttctttt
     4561 tccgacaacg gtgtgcctgg tgcaatcagg caatggacaa tgtcgcgacg aagaatacga
     4621 taaatctgct gattgacagg ctgtgtggga ttaagttgcg tttcgacggt cattcgttct
     4681 tgcttgtgag tgagttaact gcatgagtct accacttttt tgtgacaagc aataacccgg
     4741 cagttacgcc gggtataaaa attactgacg gtggacattc agtccggcat agctttgggt
     4801 aaccggcatc atttccaggg tattgatatt gacgtgagca ggcagcgttg acacccacca
     4861 gacggcttcg ctgacatctt ctggcgtcaa tgcaacggta ttttgatagg ttttttccgc
     4921 tttaccgtca tcgcctttaa agcggacatt ggaaaactcg gtgccaccca ccagacccgg
     4981 ttcgatgtcg gtgacgcgca ccgccgtacc atgcaggtcc gtacgcagat tcaggctaaa
     5041 ctgacgaaca aacgctttcg tcgcaccgta aacgttacca ccggcataag gccagctacc
     5101 tgccgttgag ccaatgttaa taatatgacc atgattacgt tcaaccatac ccggtaagac
     5161 ggcgcgcgtc atatatacca ggcctttgtt gttggtatca atcatcgttt cccagtcttc
     5221 aacgctggct ttatgcgcag gctccatgcc caacgccagg ccggcattat ttaccaggat
     5281 atcaatattg caccactcgg cagggagcga tgccagcatc tcttcaatag cggcgcggtt
     5341 gcgaacgtcc agttgggcga tatacagatt atctcccagt tcgtctttta actcctgcaa
     5401 ccgctcctgg cgacggccag tggcgataac tttatgccct tgttgaataa aacgacgagt
     5461 aatgcattca ccaaaacctg ccgttgctcc agttactaaa acgatcatct cactgttcct
     5521 caacgctttt gtgtgtcaat aacatagcac gtgatgctta agagcgggta atactggttt
     5581 gcaagattgc actccgttat cagtaagcct acgctatgga acacctcgcg ttcaggagaa
     5641 gaaaatgaca acaatgaatc ctttccttgt gcaaagcaca ctgccgtatc tggctcctca
     5701 ttttgatcaa attgccaatc atcactatcg cccggcattc gatgagggaa tgcagcaaaa
     5761 gcgggcagaa attgctgcca tcgcgcttaa cccgcaaacg cctgatttca acaatactat
     5821 tctggcactg gaacaaagcg gagaattact tacccgcgtt accagcgtct tttttgcgat
     5881 gactgcggcg cataccaatg atgaattaca gcgtcttgat gaacagtttt ccgctgaact
     5941 ggcggaactg gctaatgata tctatctgaa cggtgaatta ttcgcgcggg tagatgctgt
     6001 ctggcagcgc cgtgaatccc tggggcttga tagtgaatcc atccgcctgg tggaggtgat
     6061 tcatcaacgt tttgtccttg ccggagccaa acttgcgcaa gctgataaag caaaattaaa
     6121 agtactgaat acagaagctg cgacactgac cagtcagttt aaccagcggt tactggcagc
     6181 aaataaatcc ggcggtctgg ttgtgaacga tatcgcgcag ctggcaggaa tgagtgagca
     6241 agagattgcg ctggcggcag aggcggctcg cgagaaaggt ctggataaca aatggctgat
     6301 tccgctgctg aataccaccc aacaaccggc gcttgccgaa atgcgcgatc gtgcgacgcg
     6361 tgaaaaactg tttattgcgg gctggacgcg agcggaaaaa aatgatggca atgatacccg
     6421 cgctatcatt caacgtctgg tggagatccg tgcacaacag gcgacattac ttggttttcc
     6481 tcattatgcc gcatggaaaa tcgccgatca gatggcaaaa acacctgaag cagcgctcaa
     6541 ctttatgcgg gaaattgttc cagcggcgcg tcaacgtgcg agcgatgaat tagcctccat
     6601 acaggcggtt attgataagc agcagggcgg gtttagcgcg cagccgtggg actgggcatt
     6661 ttatgccgaa caggtacggc gggagaaatt tgatcttgat gaggcgcagc tcaagccata
     6721 ttttgaatta aacacggtgt tgaatgaagg tgtattctgg accgcgaatc agctcttcgg
     6781 tattaagttt gtcgaacgtt ttgatattcc tgtctaccat cctgacgttc gtgtgtggga
     6841 aatttttgat cataatggcg tgggactggc gttattttac ggtgatttct tcgcccgtga
     6901 ttcaaaaagc ggcggtgcat ggatgggcaa ttttgttgag caatcaacgc ttaatgaaac
     6961 gcattcggta atttataacg tttgcaatta tcagaaaccc gctgccggtg agcctgcgtt
     7021 gttactctgg gatgatgtca taaccttatt ccatgaattt ggtcatacgc tgcacggcct
     7081 ttttgcccgc cagcgttatg ccacgctttc cggcaccaac acgccgcgtg attttgtcga
     7141 atttccgtcg caaatcaacg aacactgggc aacgcatccg caggtattcg ctcgctacgc
     7201 ccggcattat cagagcgggg cagcaatgcc tgacgaactg caacagaaaa tgcgtaatgc
     7261 cagcctgttc aacaaagggt atgagatgag cgaactgctt agcgccgcac ttctcgatat
     7321 gcgctggcat tgcctggaag aaaacgaagc aatgcaggat gtcgatgatt ttgaattgcg
     7381 ggcgctggtg gcggaaaata tggatcttcc tgctataccg ccacgctatc gcagcagtta
     7441 tttcgcccat atttttggtg gcggatatgc cgcaggttat tacgcttatc tgtggacaca
     7501 aatgttggcc gatgatggtt atcagtggtt tgttgagcag ggcggattaa cgcgtgaaaa
     7561 tgggctgcgt tttcgcaagg cgatcctttc cagaggtaac agcgaagatc tggaacgcct
     7621 gtatcgacaa tggcgcggta aggctcctca gattatgccg atgctgcaac atcgtggctt
     7681 gaacatataa acaccaccaa tggctgtcct ggagatatcg ccaggccagc ctggttatag
     7741 cagtaattgc agcagctggg cgagggcaaa cctcaccgct aaagctaata ccgtttcgca
     7801 gtcgccagca aaatgcataa ccgcagtata ggtctggcct ttaatatgcc acgcgaacca
     7861 gacggtaccc gctggcgtac catcttcacc gccctccggt ccgccgtagc cggtaatggc
     7921 aatacttaca tcagcatccg cacgctctat ggctccggtt gccatttctg ctgccacttt
     7981 ctcactcacc gcagaatatc gttcaagaga ttgctggctt acgctgagga ttttcatctt
     8041 tgcctgatcg gtgaaagtaa caaagcctgc accgtaaaat ttgggtgtat cttcagctgc
     8101 acacagggcg ctagccaact taccgccggt gcatgattcg gcggtagtta agcgtaattg
     8161 atccgcgata agtctttgac tcaacgcgga tgtcaggttt tcaatagtgt cggtatctgc
     8221 gagttgtaca attttatctt tattaatgtt cactgttttt tgtccacgct attatatgta
     8281 aaggtgattt gctttttctg tatgctcg
//
LOCUS       DAZGJW010000184         8269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZGJW010000184 DAZGJW010000000
VERSION     DAZGJW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.184"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            120..821
                     /locus_tag="ACHKIK_004426"
     CDS             120..821
                     /locus_tag="ACHKIK_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9085469.1"
                     /translation="MEALQTTPKTCSLNTYNKVLCDDLDLDSFALTIANLLSAVRTFN
                     LLDDTRLKEVGFDVLEFTHEYALAIASTKQQHSIRSGNKIACIRTKREACGLTTTELA
                     RLLDLDEEIILQWESGEFEPTISMLIPLANVLGCDPLSLLSEKNSESAIRVNTTEVHA
                     ESIGARIKSARTKLGLTESDLARMIHTYSDPINDWECGICEVPADHIVPLASALNCDL
                     MWLLTGKSEAKEQQQ"
     gene            818..1018
                     /locus_tag="ACHKIK_004427"
     CDS             818..1018
                     /locus_tag="ACHKIK_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085470.1"
                     /translation="MTGNIHDKYEGLCLAPDSFANNIHNLLCAVIVLQMSDNDAIKRT
                     GDEVLEFARCYAEAAAEKELSS"
     gene            1217..1398
                     /locus_tag="ACHKIK_004428"
                     /pseudo
     CDS             1217..1398
                     /locus_tag="ACHKIK_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1404..1976
                     /locus_tag="ACHKIK_004429"
     CDS             1404..1976
                     /locus_tag="ACHKIK_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085471.1"
                     /translation="MQYAEFQAEATANGIQTGSMTIDYHDAIRRLDAGEFDTPNVRGL
                     RILQCLAQADEAGLLGKLPVEMKVAQWRWLYVTTFINEEEDKNGTIDVPNEHGTTDRA
                     VIYNGKHGVMTIYPGPIRFALQQYIEWNLIQKYGEAEGMGRALFLYQKMLITYPDKGF
                     IVSDMGREGLELLLDEMINDLNTHGMPEGQ"
     gene            2161..2349
                     /locus_tag="ACHKIK_004430"
     CDS             2161..2349
                     /locus_tag="ACHKIK_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085472.1"
                     /translation="MRLYFTIVILLAIIACIYGLLVPFLISMKDTIAVTSGFALAFLT
                     PPCIYAIYKGLSFTKDKK"
     gene            2346..3173
                     /locus_tag="ACHKIK_004431"
     CDS             2346..3173
                     /locus_tag="ACHKIK_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO9085473.1"
                     /translation="MKKIIFALAIVLPTIGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITATDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGVQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKALAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSGANTPFIQIK"
     gene            3214..3585
                     /locus_tag="ACHKIK_004432"
     CDS             3214..3585
                     /locus_tag="ACHKIK_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO9085474.1"
                     /translation="MNNQNTQPQIMNYDPNLTSCGRMAKQTVRLTFGLWEYRETFEVT
                     VGGNLTGLDVINCAIESLYATLPYEEVLDERTGKTDIMATINIGELICQDEDLSGELW
                     LAGMLISAEIISIEPATNIRL"
     gene            3617..3721
                     /locus_tag="ACHKIK_004433"
                     /pseudo
     CDS             3617..3721
                     /locus_tag="ACHKIK_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            3777..4031
                     /gene="xisR"
                     /locus_tag="ACHKIK_004434"
     CDS             3777..4031
                     /gene="xisR"
                     /locus_tag="ACHKIK_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO9085475.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            4065..5351
                     /locus_tag="ACHKIK_004435"
     CDS             4065..5351
                     /locus_tag="ACHKIK_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9085476.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEIGSN
                     AFSRYQSVVRNMLPRIGEKRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPIKGRSVV
                     TVNYYMTTIAGMFQFAADNGYIVSNPFNGLTPLKRSRTEPDPLTRDEFIRFIDACHHQ
                     QTKNLWSLAVYTGIRHGELISLAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRV
                     IHLVQPAVDALKSQAEMTRLGPQYQIDVKLREFGRTARHECTFVFNPQLVKKCQQVGH
                     HYKADSIRDSWASALRRAGLRHRKAYQSRHTYACWALSAGANPSFIANQMGHANAQMV
                     FNVYGAWMKDNNIGQIELLNKQLTESVPYMPHRARL"
     gene            complement(5825..6060)
                     /locus_tag="ACHKIK_004436"
                     /pseudo
     CDS             complement(5825..6060)
                     /locus_tag="ACHKIK_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6231..6881)
                     /locus_tag="ACHKIK_004437"
     CDS             complement(6231..6881)
                     /locus_tag="ACHKIK_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO9085477.1"
                     /translation="MPFSFNLSSGNYLSTQDVEVLQRATRDHQMERLTIGERSFSVRY
                     QSAMDAFIVDPVQGELYSGLSHTELADIIRLADSVENQLNGGNSFLDVFSTYMGQVIS
                     EFMHSNDNRIELLQRRLHSCSFLVNIEEMSYIDEALQCPITLAIPQRGVFLRNAEGSR
                     VCSLYDEMALSRIINDGMHHPLSREPITLSMLVAREQCEFDCSIGHFTVRSDCYSV"
     gene            7113..8169
                     /locus_tag="ACHKIK_004438"
                     /pseudo
     CDS             7113..8169
                     /locus_tag="ACHKIK_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         2557 a         1871 c         1756 g         2085 t
ORIGIN      
        1 atccatcaca aaaaaatcaa gtttatcaat ttttaattga cagtcgtttt cctgacacat
       61 aatatctcac catcaattat atattgatta acttcaactt agaattgcat ggtgatgata
      121 tggaagcctt acaaacaaca ccaaaaacat gtagcctcaa tacttacaac aaggttttat
      181 gtgatgattt agatctcgat tcttttgcat taaccatcgc aaacctgctc agtgctgttc
      241 gcaccttcaa cctcctagat gatacgcgat taaaagaggt tgggtttgat gtgctagaat
      301 ttactcatga atatgcttta gcgatcgcat cgacaaaaca acaacattct atccgatcag
      361 gcaacaagat agcctgcata cgcaccaaac gtgaagcctg cggcttgacg actaccgaac
      421 tagccagact actcgatctt gacgaagaga ttattctgca atgggagagt ggagagttcg
      481 agccaaccat cagcatgctt atccccctgg caaacgtcct gggatgcgat ccgctttctc
      541 tactaagtga aaaaaacagc gagtcagcta ttcgtgtaaa tacgactgaa gtccatgcgg
      601 aaagtattgg cgcacgcatc aaaagcgccc gtacaaagct gggattaacc gaatctgatc
      661 ttgcccgcat gattcatacc tacagtgacc ccataaacga ctgggaatgc ggcatctgtg
      721 aagttcctgc tgatcatata gtaccactgg ccagtgcgct taattgtgac ctgatgtggt
      781 tgttaacggg aaaatcagaa gcaaaggagc agcaacaatg actggcaata tccatgataa
      841 gtatgaaggc ttatgcctgg caccggattc ctttgcaaac aatatccata atttattatg
      901 cgcagttatc gtattacaaa tgtcagacaa cgacgcaata aaaagaacag gtgatgaagt
      961 tcttgaattt gcacgttgct atgctgaagc agcagctgaa aaagaactat ccagttaaat
     1021 agaacaagtc atctccagat actatattac ggcttaatcg ccggggatta tcacacactt
     1081 aatccacagg aggttttatt atgactttta taaaacataa ggcatcacac aaaacagcct
     1141 gccttattgc acaacacggg aaaaatcaca tgcatattgc ctgcttgttt ctgcgtaaag
     1201 catacgggag ataataatgc atcagaaaac agcagaacac gaacaaacca gagtattgct
     1261 gaccatcaaa aacgggaaag taatattaat tcgtcatgtt catgacgatg aatttgtaga
     1321 aatctttcaa cattcctgtt tattgcagaa aaggcaggat atgacgttat tgcaccagca
     1381 gatgaagatg aggaataaat ttcatgcaat acgctgaatt ccaggctgaa gcaacagcca
     1441 atggtataca aacaggcagt atgactattg attatcacga cgccatacgc cgtctggatg
     1501 ctggagaatt cgatactcct aatgtgcgag gtttacgtat ccttcagtgt ctcgcgcaag
     1561 ctgacgaagc aggattactg ggaaaacttc cggttgagat gaaggttgcc cagtggcgat
     1621 ggttgtatgt gacgacattc atcaacgaag aagaagacaa gaacggcaca attgatgtcc
     1681 cgaatgaaca cggaacaaca gatcgcgccg taatatataa cgggaagcat ggggttatga
     1741 cgatatatcc cggccccatt cggtttgcct tacagcagta tattgaatgg aatttaattc
     1801 aaaaatacgg cgaggcagaa ggaatgggta gagcgctgtt tctttatcag aaaatgctca
     1861 ttacataccc agataaaggt ttcattgttt cagacatggg gcgagaaggg cttgaactcc
     1921 ttctggatga aatgattaac gacctgaata ctcatggtat gccagaaggc cagtgacaca
     1981 ttaaatatta agaagaatat aattcttccg ttttttacta accatttata tgaaaaacaa
     2041 ccgtgaatta agcagagtaa aactgctttt aatccttgcc acattactga caataacaga
     2101 aatcattatt ctctttattg cgctgtctgt cagttaaaaa tatcgggata ccacatacca
     2161 atgagactgt atttcacaat agtaatttta ctggcaatta tcgcatgcat ttacggatta
     2221 ctcgttccgt tccttatatc catgaaggat acgatagcag ttacttctgg ctttgcactg
     2281 gcgtttctga ccccgccctg catttatgcc atttacaagg gtctttcttt cactaaggat
     2341 aaaaaatgaa aaaaattatt tttgctttag ccattgttct gccgaccatt ggccttgtcg
     2401 gctgcgatcg cgttgaaccc ggtaatgttg gcatcaaagt aaataaactg ggcgacgata
     2461 aaggcgtcgg tgaggtggtc ggtgttggtc gctactggac tggctggaat actgaagttt
     2521 acatcttccc aaccttcaaa caaatgaaga cctacgatga gccgttcagc ttccagatga
     2581 gtgacggcac aaccatcggc tatcacatcg gtgttgccta caaagttgat ccatccaaag
     2641 ttaccacggt gtttcagacc taccgcaaag gcgtggatga cattaccgcc actgacctgc
     2701 gccagaagat cgccgacgca ctcaatcgac tggccagcaa aatgaccacc gacaaattta
     2761 tcgacggcgg caagtctgaa ctactggatg ccgcacttaa agacattcag gaagagatga
     2821 cccccatcgg cgttcaggta atgagcctct cttatgtagg taaaccggaa tacccgccaa
     2881 ccgttatcga cagcattaac gccaaagtca cggcaaacca gaaaaccctg cagcgcgaac
     2941 aggaagtcaa gcaacgtgaa gcggaggcca acatgctgcg tgcagaagct gccggacagg
     3001 ctgatgccat tcgaacaaaa gccctggcag aagccgatgc cattcgttta cgcggcgaag
     3061 ctctgcgcca gaaccctggc gtcatggagc tggaagccat caacaagtgg aacggtacac
     3121 tgccgcaata catgaccagt ggtgccaata caccatttat ccagattaaa taacttacac
     3181 gcccggcaaa ccgccgggtt aatggaaaat cagatgaaca accagaatac tcaaccgcaa
     3241 ataatgaact atgacccgaa tctgacgtcg tgcggacgca tggcaaaaca aaccgttcga
     3301 ttaactttcg gactatggga ataccgcgaa acattcgaag ttactgtcgg cggcaatctg
     3361 accggactgg atgttatcaa ttgcgctatt gaaagcctgt acgcaacgct gccttatgaa
     3421 gaagtcctgg atgagcgcac agggaaaacg gatatcatgg ccaccattaa tattggcgaa
     3481 ctgatatgcc aggatgaaga cctgtccggg gaactctggc ttgccgggat gcttatctca
     3541 gcagaaatta tcagcattga acccgctaca aacatacggc tctgaagttc tcactattca
     3601 gagagcagga gaaaaaatgt tcgctctgat taatcaggga caactgtata ccgacagtgc
     3661 cggttacccg gtaaaaattg ttcgctgcat aaacaacacc gtgttgtaca gaagatgtga
     3721 agcgaatgtt tgaagtgtgg tgccactgcc gtggatgaaa gttttatgag gttggcatgc
     3781 agacaatcat ctatcagata acccccagca aatggtgtac ggagagagtc ctcattgcat
     3841 caacagggct aaagcctggc accatcgagc gagccagaag aaagtcatgg atgcagggaa
     3901 aagaataccg ccattacgct gtagaaggtg atccggggca ttacagtgaa tgcctgtaca
     3961 acatcgaaga aattatgcga tggatcgaaa accagaaaca accaggtgcc aaaaatgcaa
     4021 gttccggtta acctgttaat gctcctggac gtctgggagg ttttatgagt aacgcatcat
     4081 acccgacagg cgttgaaaac catggaggat cactccgtat atggtttcac tataatggca
     4141 aacgtgtcag agaaaacctc ggtgttcctg acaccgccaa aaaccggaag atcgctggtg
     4201 agcttcgcac ttctgtttgt tttgcaatca gaatggggag tttcgactac gccgcgcagt
     4261 tccctaattc ccctaacctg aaacactttg gtctgggaaa aagagagata accgttaagg
     4321 cactttcgga aaaatggttg gaccttaaga aaattgagat aggaagtaat gcattcagtc
     4381 ggtatcaatc cgtggtgaga aacatgcttc ctcgcatagg ggaaaaacgt cttgcttcgt
     4441 cggtaacaaa ggaagattta ctgtttatca ggaaagattt attgaccggg tatcataatc
     4501 tttctaacgg aaaaacaacg ccgattaaag ggaggtcagt agttacggtt aattactaca
     4561 tgacgacaat tgcaggaatg tttcaatttg cggctgataa cggctatatc gtgtcaaacc
     4621 catttaacgg cctgacacca ttaaagagat ccagaacaga accagatccg ctcacacgtg
     4681 acgaatttat tcgttttatt gatgcctgtc accatcaaca aacaaaaaac ctgtggtcct
     4741 tagcagtata cacaggcatt cgtcacggtg agctaatatc tctcgcctgg gaggatattg
     4801 atttaaaagc taaaacaatg actatccgtc gtaattatac aaaactcggg gagttcactc
     4861 taccaaaaac agaagcggga actgatcgtg ttattcatct tgttcaacca gctgttgatg
     4921 ccctgaaaag ccaggctgaa atgacaagac ttggccctca gtatcaaatt gacgtcaagc
     4981 ttcgggagtt cggtcgcacc gcacgccatg aatgcacgtt tgtttttaat ccgcaactgg
     5041 taaaaaaatg ccagcaagtc ggtcaccact ataaagcaga ttccatcaga gattcctggg
     5101 catctgcatt aaggcgagca ggactgcggc acagaaaagc ctatcagtct aggcatactt
     5161 atgcctgctg ggcattatcg gcaggagcga atccaagctt catagcaaac caaatgggcc
     5221 atgcaaatgc acaaatggta ttcaacgttt acggagcatg gatgaaagat aacaatatcg
     5281 ggcaaataga actactcaat aagcagttga cggagagtgt cccatacatg ccccatagag
     5341 ccagactctg aaataatatc tttaaatatc agttgtttta gtggtagttg ccgtcacatt
     5401 aactgcatga cagggctatt taaacaatca ctaaaaatcg cccaagagca ttctgtttct
     5461 ataatgtatt ccaagagatt aaaaaataat atcatacgtt attcggcaga gttcgtatgt
     5521 actacggcgt cccaaccatg tcccaaaaca aaaatttctg ccctatacac gttccaaaaa
     5581 attcaacagc aaagagagtc tgctgacgag ttgttgattc actgattaat acgtaaaatt
     5641 gataactgct ggaaatcata caatactcgt actttcgaaa gttcgctaac caggtgctac
     5701 acgcgaggtg ttgcagtttt ctttacgatt tatcattcaa tcctaatcca tgcatgacat
     5761 gtggtgcctg tggggtgatc ctacctacgt aatgtggaca cagccctaat caaggttctg
     5821 tttttcaaaa cagttccgtg ctgagacagc cacaagtact gtgtcggcac caccgattgt
     5881 aatcacacta gatctaatta aacacagccg tctgcattat ttcccgactc ataaaatatt
     5941 agccgtaaat gcattcgact tttagtgagt ggaagaaact ttccgcccta gcattatcgt
     6001 aacagcgccc ctttgcactc atcctgtcat gcaggtatgc cgcttcagaa gcgcctgata
     6061 atgcgttgaa atcaggacgg tggctacttg ctattcagtt cacgatacaa aatgggcaat
     6121 atcccttaag gaccagcgaa tagcaatgag tcactttatt atcaagtttg atgaccgctt
     6181 tgatgctaac ttttaataag agtctcttca cacaaaatcg tgtaccgagt ctacactgaa
     6241 taacaatcac tcctcaccgt aaagtgaccg atactgcaat caaactcaca ctgctctctg
     6301 gccacaagca ttgataatgt tattggctct ctgcttagtg ggtgatgcat cccatcatta
     6361 attatacgag aaagagccat ttcatcatat aaactacata ctctggaacc ttcagcattt
     6421 cttaaaaaaa cacctcgttg aggaattgcc agcgtaatcg ggcactgtaa tgcttcatct
     6481 atgtaagaca tttcttcaat attaactaaa aatgaacatg aatgtaatcg ccgctgtaac
     6541 aattcaattc tgttatcatt actatgcata aattcagaaa taacctgccc catatatgta
     6601 ctgaatacat caagaaatga attgcctcca ttcaattgat tttcaacaga atcagccaat
     6661 ctaatgatat cggctagttc tgtatggctt aaacccgaat atagttcccc ttgaacagga
     6721 tctacaataa aagcgtccat agcactctga tagcgaacag aaaacgatct ttctcctatt
     6781 gtgagtcttt ccatttgatg atctctggtc gcacgttgta aaacttcaac atcttgtgtt
     6841 gacaagtaat ttccagaaga caaattaaat gaaaatggca taattttatc cctaaagcat
     6901 atatcttact tttcgataat taacaaaaag ccatcctcca cagcaatact ccatcagaat
     6961 aattgtagga ttattttaat ctaatttata agcataaaat atatactcac taaatagctg
     7021 cgcagaatag tagatcattt agagggaact caacccgatt gtgcgatctg atcaatcgcc
     7081 aaatcaatac aaatcaccaa ccggactgag caatgtcgat cataccacca atttcccgtg
     7141 acgaacgacg cctaatacag aaagccatcc ataaaaggct gacagccatg ctgatgctgc
     7201 accggggcga ccgtgtcagc gacgttgccc gcacactatg ctgcgcccgt tcctctgttg
     7261 ggcgctggat taactagttc acacagtcgg gtgttgaggg actgaaatca ttacctgccg
     7321 agcgagcccg ccgctggccg tttgcgcata tctacacact attacgtgaa caggtaaaat
     7381 attcactcgc gactttggct acaattattc acgctggagt acagaactgc tgacaataaa
     7441 aatcaatgag ataaccggct gcctgttaca tgctggaacc attcgccgct ggttgtcgtc
     7501 tgtggggctt gtgtggcgaa gggctgcgcc aactctacgt atccgtgccc cgcctaaaga
     7561 taaaaagatg gcaacaatcc ataacgcact ggacgaatgc agcacagagc atccggtctt
     7621 ttatgaagat gaagtattta tccatcttaa tcccaaaatc ggtgcggact ggaaactgct
     7681 cgggaagcaa aaacgagggg tcacgccgga acaaaatgaa aaatattctc tggacgtagc
     7741 gctgcacagc gggacaggtt aagtcagctg tgtgggcgcc aacagcaaaa gttcggcgct
     7801 gttcatcaga ctgctgaagc ggcttaaagc tacatactgt cggacgaaaa ccatcacgct
     7861 gctcgaggac aactacatta tccacaaagg ccgggaaacc cagcgctggc tgaaggataa
     7921 accgaagtgc agagtcattt atcagccggt ttaatctcca tgggtgaatc attttgaata
     7981 gctatggcag atacttcacg ccacaataac gcgtaatcat cagtgccgct caatgtggca
     8041 actattgaaa aagttcgcca ttttatggaa tccgtcagtc cattccccga aggcaaacat
     8101 gggctggcaa aaatgtgcga tattaggcgc agctatttag ttattccgtc atttctcccg
     8161 cccgtgtaaa cactactgaa tcttgtcgtt gtgacaaagc tataaatagt caatcacagc
     8221 acgtttctgc gtacgacgcg ctgcaatttt ctttacgatt tataactgt
//
LOCUS       DAZGJW010000185         8257 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZGJW010000185 DAZGJW010000000
VERSION     DAZGJW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.185"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1508
                     /locus_tag="ACHKIK_004439"
                     /pseudo
     CDS             1..1508
                     /locus_tag="ACHKIK_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(1477..3213)
                     /gene="aceK"
                     /locus_tag="ACHKIK_004440"
     CDS             complement(1477..3213)
                     /gene="aceK"
                     /locus_tag="ACHKIK_004440"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO9085478.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPHFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3494..4798)
                     /gene="aceA"
                     /locus_tag="ACHKIK_004441"
     CDS             complement(3494..4798)
                     /gene="aceA"
                     /locus_tag="ACHKIK_004441"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO9085479.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4828..6429)
                     /gene="aceB"
                     /locus_tag="ACHKIK_004442"
     CDS             complement(4828..6429)
                     /gene="aceB"
                     /locus_tag="ACHKIK_004442"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO9085480.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6698..7627)
                     /gene="metA"
                     /locus_tag="ACHKIK_004443"
     CDS             complement(6698..7627)
                     /gene="metA"
                     /locus_tag="ACHKIK_004443"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO9085481.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7784..8227
                     /locus_tag="ACHKIK_004444"
     CDS             7784..8227
                     /locus_tag="ACHKIK_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO9085482.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2125 a         2040 c         2038 g         2054 t
ORIGIN      
        1 accaaaccct gagtgaattg ttttataaag aactcgagga taaattttct ggcaaggagc
       61 tggcgacgcc tctattaaaa agcttctcag agaactgtag acaaaatggt cgtcatatct
      121 ttagcaacaa ggattttgtc attaaatttt ccacgtccgt cttacaagct gataagaaag
      181 aaattacgat aattaataaa aacgaaaaca cgacacttac tcaaaccatt gccccaatat
      241 ttgaagaata cctaatggaa attttacctc aacgctcaga cactcttgat aaacaagaat
      301 taaacctaaa atcagataga aaagaaaaag aattcccaag aattaaactt aatggtcaat
      361 gttattttcc ggggcgaccc caaaaccgta tagtatgccg acacattgct gcacaatata
      421 ttaatgatat ttatcagaat gttgattaca aaccccatca agatgattac tcttcagctg
      481 aaaaatttct cactcacttc aacaaaaaat gcaaaaacca gactttggcg ttgatttcca
      541 gccgtcctga ggggcgttgt gttgctgcct gtggtgattt cgggctagtt atgaaggcat
      601 atttcgacaa gatggaatca aatggcatca gtgttatggc agccatatta ctggtggata
      661 accatgcttt gacggtccgg ctaagaataa agaacacaac tgaaggatgt acccattacg
      721 tggtttcggt ttatgatcct aatgtcacta acgataaaat aagaattatg agcgaaagca
      781 aagaggatat taaacactat tctctgatgg attttatgaa tgtagattat agcctcctga
      841 aatggtcaaa tgatcatatt attaaccaat ctgttgcaat aattccagca cttccgaaag
      901 aacagctatt gatgttaaaa ggatctgtgg atgaaataac ccctccatta tcaccagcaa
      961 cgatgaattt gctaatggca attggtcaga atcaccaact tacgcaactg atgattcagc
     1021 tccagaaaat gccagaacta catagaacag aaatgttgac tgcctataat agtggacata
     1081 tgaacgttat taatactatt tttaacgcat aacccactct gtttaatacg tttaaattcg
     1141 ataaaaaaaa tatgaagccc ctcctcctgg caaataattc taatgaatat cccggtttgt
     1201 tttcagcgat acagcataaa caacaaaatg ttgtagagac ggtttatctt gctttatcta
     1261 accatgcacg cctgtttgga tttaccgctg aagatattat ggatttttgg caacacaaag
     1321 ccccacaaaa atactctgcc tttgagttgg cttttgaatt gggtcaccgg gttattgctg
     1381 aattaatcct taatacatta aataagatgg ctgaaagctt tggctttacg gataaccctc
     1441 gatacattgc ggagaaaaat tatatggaag ctttactcaa aaaagcatct ccccataccg
     1501 tacgctaaat ctttgcctgc gccgatacgc ataaacatct tccacatgcc cttcacgtat
     1561 gcggttttgt agtgcgcgcc agtaatcagc gcggaacagg tcggcgtgca tctcttcaaa
     1621 cagcggacca atacgcgggt cggcgcatag ccagtggcga aactcttccg ggaaaacatc
     1681 gcccggcgag acgctgtacc acggttcgct ggcaagttcg tcttccggat agcgcggcgg
     1741 cgggatgtcg cggaaattca cttccgtcat gtagcaaatt tcatcgtaat cataaaaaac
     1801 cacacgcccg tgacgggtga caccgaagtt tttaaacagc atgtcgccag gaaaaatgtt
     1861 agcagcggca agctggcgaa tagcgttacc gtattcttca atggcgtcgc gcaactgctg
     1921 accttccact tgttccagcc agatattgag cggcaccatc cgccgctcga tatagagatg
     1981 gcgaattaca atctgctcac caagatcggt gattttttcc gctgcttcct ggagcagtaa
     2041 ttccattaat gccggggaaa tatgccgctt ctccagcaca aagttttcaa actcctgggt
     2101 gtccgccatt cggcccacac ggtcgtgctc tttcaccagt tgatagcagg cacgaacgtg
     2161 agcggcagac atctctttct gcggcgcgaa cttgtctttg atgactttga atacccgatc
     2221 aaagcccggc agcgtaaata ccaacatcac cattccacga atacccggcg cttcaatgaa
     2281 ctgctcatta cagccctgta gataaacgag atattcgcgg tagctttcgg ttttggcgtg
     2341 cttctggcag ccgatagcca tatacaattc agcagtggtt ttacccggca gaatttcccg
     2401 tagccactcg accagcgctg cgggcagcgg tgcataaacc ataaaataag aacgcgcaaa
     2461 gccaaaaaca atgctcgctt cggcggtcgt cgtcaggcag gtatcaataa ataactcgcc
     2521 gtcgtccgtc tggtggatcg gcagtaagaa gggcaacgtg ccggaaggcg tgatcagttt
     2581 gcccaccagc caagcggctt tattgcggta aaacagttcg ttcgccacct gtaaatgact
     2641 ttccgcgagg ttgtctgtcc ccagcgtttc cgtcagatgg cgaatgatgt aatgaatgtc
     2701 acggctttta ttctgccagc gcaggcgcag cggcaggtcg ctgataacgc gcatcagtag
     2761 agattcccag ccgtgatcgg ggtgaaagtc tttcgccagc gggcggggaa tggtacgaaa
     2821 gcggcgctct ggctgagagc taaagataaa aagccgctcg ggagtaagcg agcggtggtc
     2881 aaataaccga cagtacacgg agttaaaaaa gctctccgca atctcgaagt gcgggtaatc
     2941 cggcaacagc cgcgtgtaat gctctttaac gcgcagtaaa aatgccgcgt ccgtactttg
     3001 gccgttagta atgcagcgca gttgctccac gaccagacca acgtgatgat cgtaaagatg
     3061 gatacggttt ttcatcgcct gctggacagc atgccagtcg gcctgttcga aacgctgctg
     3121 cgccccggaa gtcacttcga ggaatcgacc atactgagcg tcgaagcctt gcaaaatggt
     3181 ttgagcaatc agtaattcca ggccacgcgg catttagcgc cctcatcagg agcagagaat
     3241 tgccggatgc ggcgtaaacg ccttatccag cctacgttcg gcaacggttg taggcatgat
     3301 aagacgcgca agcgtcgcat caggcatcgt gcaccgattg ccggatgcgg cgtaaatgcc
     3361 ttatccggcc tacgttcggc aacggctgta ggcatgataa gacgcgcaag cgtcgcatca
     3421 ggcatcgtgc accgattgcc ggatgcggcg tgaacgcctt atccggccta cagtcagcaa
     3481 cggttgttgt tgcttagaac tgcgattctt cagtggagcc ggtcagcgcg gtgactgaag
     3541 acgtgccgcc ctgaataata gtcgtcactt tatcgaagta acctgtaccc acttcctgct
     3601 ggtgagatac gaaggtatag ccatctttcg cggcggcaaa ttccggctgc tgcactttct
     3661 caacgtagtg cttcataccc tcgccctggg cataggcgtt tgccaggtca aacatgttga
     3721 accacatgct gtggatacct gccagggtga tgaactggaa cttgtagccc atatccgaca
     3781 gctgctgctg gaagctggca atagttttgt cgtcgaggtt tttctgccag ttgaacgacg
     3841 gcgagcagtt ataagccagc agtttgcccg gatatttcgc gtggatagct tgtgcaaagc
     3901 gacgcgccag ttccagatcc ggcgtggagg tttcacacca gaccaggtca gcatatggcg
     3961 catacgccag gccacggctg atcgcttgct caatgcccgc atgagtacgg aagaagcctt
     4021 cactggtacg ctcgccagta ataaattcac tgtcatacgg gtcgcaatcg gaggtaatca
     4081 gatccgccgc atcggcatcg gtacgagcaa ccagcagggt tggaacgccc gtcacgtcag
     4141 ctgcaagacg cgccgcgacc agtttctgaa tagcttcctg agttggcacc aaaactttgc
     4201 cgcccatgtg accgcatttc ttcactgacg ccagctgatc ttcgaagtga actgccgctg
     4261 caccggcttc aatcatcgct ttcatcagtt caaaggcatt caggacaccg ccaaaaccgg
     4321 cttccgcatc ggcaacgatc ggcaggaagt aatcgacata gcgcggatcg cccggctcaa
     4381 tgcctgcgga ccattggatc tgatcggcac gacggaaggt gttgttgatc cgctccacca
     4441 cagctggcac cgagtttgcc ggatagagcg actgatccgg atacatgctg gccgccaggt
     4501 tagcgtccgc cgctacctgc catcccgaca gatagactgc ttcaataccc gctttcgcct
     4561 gttgcagcgc ctgaccgcca gtcagtgcac cgaggctgtt gatgtagcct tttttcgact
     4621 cgccatgcag cagacgccac attttcgccg cgcccagttg cgccagggtg cattcaggat
     4681 tgactgaacc gcgtaatttc accacatctt ccgcgctgta tgggcgagta atgccttccc
     4741 aacgcggttg agtccactct ttctgtaatt cttcaatttg ttgtgtacgg gttttcatgt
     4801 gcagatgctc catagttatg tggtggttta cgctaacagg cggtagcctg gcagggtcag
     4861 gaaatcaatt aactcatcgg aagtggtgat ctgttccatc aagcgtgcgg catcgtcaaa
     4921 acgcccctgg gagaaacgtt cttcgcccag ttcgctggca atgactttca tctcttcacc
     4981 cagcatctgg cggaacaagg ctttggtcac cggtttgcca ttgctcaacg ttttttgatg
     5041 atggatccac tgccagattg aggtacggga aatttcagcc gtcgccgcat cttccatcag
     5101 gccataaatc ggcacgcagc cgttgccgga gatccatgct tcgatgtact gcactgccac
     5161 gcgaatgttg gcgcgcatac cttcttcggt gcgttcacca tcacaaggtg ccagcagctg
     5221 atcggcagta atcggcgcgt cttgttcgcg catcacttca agctgatttt tacgggagcc
     5281 gagaatgtcg ttgaataccg ccattgccgt atccgcaagg cctgggtgag caatccatgt
     5341 gccatcgtga ccgttattgg cttccagcgc tttatccgct tttactttgt tgagcacctg
     5401 gttattgcgc tcttcatctt tgctcggaat aaacgctgcc atgccgccca tcgcaaaagc
     5461 accgcgttta tggcaggttt taatcaacag gcgtgagtaa gcattcagga atggtttatc
     5521 catcgtcact gcctgtctgt ctggcaggac gcgatcggga tagtttttca acgttttgat
     5581 atagctgaag atgtaatccc aacgaccgca gttcagacca acaatatggt cacgcagcgc
     5641 gtgaaggatt tcatccatct ggaacacggc gggcagcgtt tcaatcagca acgtcgcctt
     5701 gatggtgccg cgcggcagat taaagcgatc ttctgcatag ctgaagactt cgctccacca
     5761 ggccgcttcc tgccaggact gggttttcgg cagatagaaa tagggaccgc tgccctttgc
     5821 caacagtgcc tgatagttgt ggaagaaata gagcgcaaaa tcaaacaggc taccggggat
     5881 tgcctcacca cgccaggtga catgtttttc cggcaagtgc agaccgcgta cccgacaaat
     5941 caaaaccgct ggattgggct tgagctgata aattttgcct gcttcattgg tatagctgat
     6001 ggtgccgtta accgcatcgc gcaggttaat ttgcccgtcg atcactttgt tccagtctgg
     6061 tgccagtgaa tcttcgaaat cggccataaa gactttcaca ttggcgttca gtgcgttgat
     6121 caccatcttg cgctcaaccg ggccagttat ctcgactcga cgatcttgta agtccgcggg
     6181 aataccacga attttccagt ccgtatcgcg aatggaagct gtttccgaaa taaaatcagg
     6241 caaagttccg ttatcaatat cttgctgctg ctgaatgcgc gctgccagaa gtttattgcg
     6301 ttgtggcgta aaatgcgtca ccagctcagt cagaaattct accgcttcgg cagtaagaat
     6361 ttgcttctcc tgctcgccat acggccttat gaaagccagt tcatcggttg ttgttgcctg
     6421 ttcagtcatc gtgcagctcc tcgtcatgga cccgaaaact tccccactgt gaacgaagga
     6481 tcgttgtgcg cttttcgttc agcacaacta agactactca tttaaaatgc aaaatcaaaa
     6541 acaatttcca ttttaattaa aaatacttga taacttattg ataacaatat gaataaaatt
     6601 tattcacctc ctgaggtgcg tttcggaaat aaaaaaggca cccgaaggtg cctgaggtaa
     6661 ggtgctgaat cgcttaacga tcgactatca cagaagatta atccagcgtt ggattcatgt
     6721 gccgtagatc gtatggcgtg atctgataga cgtaatagtt gagccagttg gtaaacagca
     6781 aattaccgtg actacgccag ctcgctcgcg gtgtattttg cggatcattg tgcgggaaat
     6841 agttatacgg tacttccggg cctagtccgg cttccacatc gcggaaatat tcctgcgcca
     6901 gcgtttgcgc atcatattcg ggatggcccg tcacaaaggc aatgcgctta tctttgctgg
     6961 caaacagata tgcatcccct tcttccgtct ctgccagaat ttccagatcg gtgtaatcac
     7021 gaatcaacgc tgccggaaag tcagcatagc gcgaatgtgg tgccaggaat gaatcatcaa
     7081 agccacgcgt cagaagcgca tgaggatgga gaatatgatg ctcgtaaacg ccagagagtt
     7141 tgtcggtgcg agtttgctta ggaatgccgt agaggatatt gagcgcggcc tgtaccgccc
     7201 agcagacaaa cagcgtcgag gtgacgtgat ctttcgacca ctccagcacc tgtttgatct
     7261 gcggccagta agcgacatca ttaaactcca ccaggcccag cggcgcaccg gttacaatca
     7321 gaccgtcaaa gttctgttcc tgaatatctt caaagttaca gtagaagttg ttcagatgct
     7381 ctgcgggcgt gttgcgcgat tcacgggaat cgatgcgcaa cagctgaata tcgacctgca
     7441 aaggtgagtt tgaaagcagg cgcagaaact gattttcagt ttcaatcttc ttcggcatca
     7501 ggttaaggat tagaacctta agtggacgaa tttcctgacc agacgcacga gaagttgtca
     7561 tcacaaagac gttttcttca cgcaagaaat tgacggcggg tagctcgtcc ggcacacgaa
     7621 taggcataac ctgattacct cactacatac gcttatacgt ttagacatcc agatagctga
     7681 agataaccag aaaatttgcc aatgtcgagc ctgcatgttg aaggtgagaa agtttcaagg
     7741 aaaaggctac gttagaatat aagaatgacg aaaaggagag aaaatggtta ttagtattcg
     7801 ccgctcacag catgaggaag gggaggaact cgttgcgatt tggtgtcgat ctgtcgatgc
     7861 cacccacgat tttttatcag cagagtatcg ggccgagctg gaagagctgg ttcgttcctt
     7921 tctgccggaa gcaccgttgt gggtcgcggt taatgaacgg gatcagccgg ttgggtttat
     7981 gttgttaagt gggcagcata tggatgcgct gtttatcgat cctgatgtgc gcggctgcgg
     8041 cgtgggtcgg atgctggtgg agcatgcgct ctcgatggca ccggaactga caaccaacgt
     8101 taatgagcaa aatgagcagg cggttgggtt ctataagaag gtgggtttta aggttacggg
     8161 acgctctgag gtggacgatt tggggaaacc gtatccgttg ctgaatctgg cgtatgtggg
     8221 ggaataagcc gcccctgccg tgcctgccca ggctgga
//
LOCUS       DAZGJW010000186         8117 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZGJW010000186 DAZGJW010000000
VERSION     DAZGJW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.186"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..271
                     /locus_tag="ACHKIK_004445"
     CDS             <1..271
                     /locus_tag="ACHKIK_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085483.1"
                     /translation="TRKDNIFILFSSADDGPVMVVSSQRLHDMLNPTKDTNWNSTCIY
                     KSRHKMLPINLTQETLFSSKSHGKYALFPIFTASWRATRIKNIGI"
     gene            494..991
                     /gene="ubiC"
                     /locus_tag="ACHKIK_004446"
     CDS             494..991
                     /gene="ubiC"
                     /locus_tag="ACHKIK_004446"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO9085484.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1004..1876
                     /gene="ubiA"
                     /locus_tag="ACHKIK_004447"
     CDS             1004..1876
                     /gene="ubiA"
                     /locus_tag="ACHKIK_004447"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO9085485.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2031..4454)
                     /gene="plsB"
                     /locus_tag="ACHKIK_004448"
     CDS             complement(2031..4454)
                     /gene="plsB"
                     /locus_tag="ACHKIK_004448"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO9085486.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4625..4993
                     /gene="dgkA"
                     /locus_tag="ACHKIK_004449"
     CDS             4625..4993
                     /gene="dgkA"
                     /locus_tag="ACHKIK_004449"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO9085487.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5103..5711
                     /gene="lexA"
                     /locus_tag="ACHKIK_004450"
     CDS             5103..5711
                     /gene="lexA"
                     /locus_tag="ACHKIK_004450"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO9085488.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5784..7109
                     /gene="dinF"
                     /locus_tag="ACHKIK_004451"
     CDS             5784..7109
                     /gene="dinF"
                     /locus_tag="ACHKIK_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO9085489.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7225..7434
                     /locus_tag="ACHKIK_004452"
     CDS             7225..7434
                     /locus_tag="ACHKIK_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO9085490.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7476..7991)
                     /gene="zur"
                     /locus_tag="ACHKIK_004453"
     CDS             complement(7476..7991)
                     /gene="zur"
                     /locus_tag="ACHKIK_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO9085491.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1822 a         1987 c         2236 g         2072 t
ORIGIN      
        1 aacacgaaag gataatatct ttatcctgtt ctcctctgcc gatgatgggc cagttatggt
       61 ggtaagctcc cagcgcttac atgatatgtt gaatcctaca aaagatacca attggaattc
      121 cacgtgtatc tataaatcca gacataagat gttgcctatt aatcttactc aggaaacact
      181 ttttagctcc aaatctcatg gtaaatatgc gcttttccca atttttactg cgagttggcg
      241 agctactcgt ataaagaata taggtattta agtaaaggaa aacatcaccg ttcctggcat
      301 catgggcggt gatgccccta cggttgccct cgccagcacg ggcatcggca aagcgtaagg
      361 ttcaacagcg gtttaccact tcatgcgatt gttgcgtttt tgttgcgcat tagatcactt
      421 aatttgcttt acatctcccg taaacacttt tctgcgatac aatgccttta cgttatgtaa
      481 cggagagttc ggcatgtcac accccgcgtt aacgcaactg cgtgcgctgc gctattttaa
      541 agagatccct gccctggatc cgcaactgct cgactggctg ttgctggagg attccatgac
      601 aaaacgtttt gaacagcagg gaaaaacggt aagcgtgacg atgatccgcg aagggtttgt
      661 cgagcagaat gaaatccccg aagaactgcc gctgctgccg aaagagtctc gttactggtt
      721 acgtgaaatt ttgttatgtg ccgatggtga accgtggctt gccggtcgta ccgtcgttcc
      781 tgtgtcaacg ttaagcgggc cggagctggc gttacaaaaa ttgggtaaaa cgccgttagg
      841 acgctatctg ttcacatcat cgacattaac ccgggacttt attgagatag gccgtgatgc
      901 cggactgtgg gggcgacgtt cccgcctgcg attaagcggt aaaccgctgt tgctaacaga
      961 actgttttta ccggcgtcac cgttgtacta agaggaaaaa aatatggagt ggagtctgac
     1021 gcagaataag ctgctggcgt ttcatcgctt aatgcgtacg gataagccaa ttggcgcgtt
     1081 actgctgctc tggccaacat tatgggcgtt gtgggtggcg acaccgggcg ttcctcagct
     1141 ctggatcctg gcggtatttg tcgcgggtgt ctggctgatg cgcgctgctg gatgtgtggt
     1201 gaatgattat gctgaccgaa agtttgatgg tcatgttaag cgcacggcga accgaccact
     1261 tcccagcggc gcggtaacag agaaagaggc gcgcgcactg tttgtagtgc tggtactgat
     1321 ttcgttttta ctggtactga cgctgaatac gatgaccatt ctgttgtcga ttgccgctct
     1381 ggcgttggcg tgggtgtacc cgtttatgaa gcggtatacc catctcccgc aagtggtgct
     1441 gggcgcggcg tttggctggt cgattccaat ggcttttgcc gctgtgagtg agtcggtgcc
     1501 attgagttgc tggttaatgt tcctcgccaa tattctctgg gcggtggctt acgacacgca
     1561 gtatgcgatg gttgaccgcg atgatgatgt gaagattggc attaaatcca cggcaatcct
     1621 gttcggccaa tacgataaat tgattattgg tattttgcag attggcgtac tggcactgat
     1681 ggcgatcatc ggtgagttaa atggcttagg ctggggatat tactggtcaa ttgtggtggc
     1741 tggcgcgctg tttgtttatc aacaaaaact gattgccaac cgcgagcgtg aagcctgctt
     1801 taaagcattt atgaataata actatgttgg tctggtacta tttttagggc tggcaatgag
     1861 ttactggcat ttctgatgat gtaaaaaagc cggatgatca tccggctttc ttctgggttg
     1921 cctgatgcgc ggcgcttctc aggcctacac aacacatcgc aatttattga atttgcagat
     1981 tatggaaggc cggataaggc gttttcgccg catccggcaa ttctctctga ttacccttcg
     2041 ccctgcgtcg cactctcaat cgtcaaacgc acgtctgatg taatcaactc cgccagcaac
     2101 tgataaacct tcatcgtttc tgccggttcg gcatcgccgc tatcgctgat atacccttca
     2161 tcacgcagtg tcagcaccag agaactgaac accgccttgt cgaagaactc cggcgcgttg
     2221 atgccgtgca gcacggagag acgttgcgcg acggtgcggc tctctttctc cagcgtaccg
     2281 cggttgatcg acgggttggc actcaacaac cagaaggtga tggcataacg ttgcagcgtt
     2341 tcgcgcgcgc ctgcggcgag cagctgtagc gtgcgagaat gcaccgggtt gatatgcaac
     2401 tcatcatctt gcagggtaat cagcccctga cgttgcatct catttgccag cgcatcaata
     2461 acgtccggca actcgtcgcg atcccagcgc aggaacagct ccgctttcag cattgggtaa
     2521 agcacattga cgtgctccat caatacgtcg cgggagatgt ggcgatgctg ggtgacaatt
     2581 gccgccatca gcgaaggcag caccaacata tgcgcaatgt tgttgcgata gtaggtcatc
     2641 agcaccgctt gctcgcgtgg cagaatgatg atgtcgccga tagtgtcttt ctcgacttca
     2701 aacttgttca tttgcagcgc gtgatcgata agctcgctgg cgctggctga aggaacggta
     2761 gagtccgtgg agtaaggtac gttgcgcatc agatccaggt agcagttgag ttgctcggtt
     2821 aactgctcgc gggtgagtga gcgctgacgt gatgccagta gcgcagtaca gcacaggttc
     2881 atggcgtttg ccgcgcctgc gttgttaatg cgtaccatca gatcggcagc aatattattg
     2941 accgtcggcg ttaaccaggc cggacgcacc gcttcgatgg gatcgataga ttcacgccag
     3001 tctggtacgt gctggttaag gtaggtcatc aacggcattg gttcaccgaa gttgacgtaa
     3061 ccctgaccga gattacgcag cttgcttaaa ccgcgcagca tctgcggcag gctctctttc
     3121 tctttcgtcg cgccgcgcag ttctttggcg taagtaccca cttccatgac gtgctcataa
     3181 ccgatataga tcggaatcag cgtaatcgga cgcgtgccgc cacgcagcat cgcctgaatg
     3241 gtcatcgaca gcgtaccggt tttcggatcc agcaaacgcc ccgtgcggga acgaccgcct
     3301 tccacgaagt actcgacgga ataaccacgg ctgaacagtt cgccgagata ctcacggaaa
     3361 acggtggaat aaagtttatt gcctttaaaa gtacggcgaa taaagaacgc ccccagacgg
     3421 cggaaaatcg gcccggccgg ccagaagttc aggttgatcc cggcggcgat atgcggcggc
     3481 accagcccct ggtggtacag cacgtaagaa agtagcaggt agtccatgtg actgcggtgg
     3541 caaggcacat acaccagctc atgaccgtcg tgggccagct ggcgaacgcg ctcggcgtta
     3601 tggacgttga tgccctgata aagtcggttc caggtgaagc ccagaatgcg gtcagtcagg
     3661 cggatcatct cgtaagagaa attcgccgca atctcttcca tcagcgcaat cgcgttctgc
     3721 tgcgcttttt catgggagat ttttttgctg cgcgcttcat cttctaccgc tttggcaatg
     3781 gcgcgggagg cgagcagctt attaaacaga tcctgacgag caggcagacg tgggcccaca
     3841 gcagccagac gttgacgggc aaagtgcata cgcgccacgc gcgccagttt ctgagcgata
     3901 gttttatctg tgccgtgttc atcggccatg cggcgcagag aaactgacgg cgagaaacgc
     3961 acaaaactgt cgcgaccgag ccacagtacg gcgaaaaatt tttgtacgcc gttaagcata
     4021 cgcagcggcg ggtttacttc gcctttttca cgccccggcg cgcgaccaaa catcaccgat
     4081 actggcacca tctgcacatc cagatttggg ttgctgcggt gcaaatcaag atagtcgtgg
     4141 aacagcttaa tagactcttc tttcggcgtg taataggtga acacacgcgg cccgccgtga
     4201 atgaacacat agcgcggcag tagcgtgccg tcgatttcca gcggctctaa cgggtcaggc
     4261 aagtcatgag ccagacactg ggcgcgcaac gtcagcaaat ctgctttcga gttgtacggt
     4321 aaaacgtaca taattggacg agaggtatcc agccccagtt ccggggcagg atctgccgga
     4381 atagacttgc tttttaccag gatgcttaat ggtaaattca gtaatttgta gtaaattcgt
     4441 ggccagccgg acataaacga tgtaaagcct ctggttaata atgcaaatgc gcggcaagga
     4501 tagcagaaag tcatgggaaa ttctgtggta tccgctcatg tttcgcgcgg cgttacgcaa
     4561 acccgaatca tcggatttaa cggtacactg atattgacgc tcataatgta aaaaggttct
     4621 ttcaatggcc aataatacca ctggattcac ccgaattatc aaagctgctg gctattcctg
     4681 gaaaggttta cgcgctgcat ggatcaacga agcggcattc cgtcaggaag gcgtagcggt
     4741 attgttggcg gtggtcatcg cctgctggct ggatgtggac gcaattaccc gcgtgctgct
     4801 tatcagctcc gtgatgctgg tgatgattgt ggaaatcctc aatagcgcca tcgaagcagt
     4861 ggttgaccga attggctctg aataccatga gctttccgga cgcgcaaaag atatgggatc
     4921 cgccgcggtg ctgattgcca ttatcgtcgc cgtgattacc tggtgcatcc tgttatggtc
     4981 gcattttgga taacccttcc agatttcgat aaatctctgg tttattgtgc agtttatggt
     5041 tccaaaatcg ccttttgctg tatatactca cagcataact gtatatacac ccagggggcg
     5101 gaatgaaagc gttaacggcc aggcaacaag aggtgtttga tctcatccgt gatcacatca
     5161 gccagacagg tatgccgccg acgcgtgcgg aaatcgcgca gcgtttgggg ttccgttccc
     5221 caaacgcggc tgaagaacat ctgaaggcgc tggcacgcaa aggcgttatt gaaattgttt
     5281 ccggcgcatc acgcgggatt cgtctgttgc aggaagagga agaagggttg ccgctggtag
     5341 gtcgtgtggc tgccggtgaa ccgcttctgg cgcaacagca tattgaaggt cattatcagg
     5401 tcgatccttc cttgttcaag ccgaatgctg atttcctgct gcgcgtcagc gggatgtcga
     5461 tgaaagatat cggcattatg gatggtgact tgctggcagt gcataaaact caggatgtac
     5521 gtaacggtca ggtcgttgtc gcacgtattg atgacgaagt taccgttaag cgcctgaaaa
     5581 aacagggcaa taaagtcgaa ctgttgccag aaaatagcga gtttaaacca attgtcgttg
     5641 accttcgtca gcagagcttc accattgaag ggctggcggt tggggttatt cgcaacggcg
     5701 actggctgta acatatctct gagaccgcga tgccgccagg cgtcgcggtt tgtttttcat
     5761 ctctcttcat caggcttgtc tgcatggcat tcctcacttc atctgataaa gcactctggc
     5821 atctcgcctt acccatgatt ttctccaata tcaccgttcc gttgctggga ctggtcgata
     5881 cggcggtaat tggtcatctt gatagcccgg tttatttggg cggcgtggcg gttggtgcaa
     5941 cggcgaccag ctttctcttt atgctgttgc tgtttttacg catgagcacc accgggctga
     6001 ctgcgcaggc ttatggtgcc aaaaatcctc aggcattagc ccgtgcgctg gtgcaaccgt
     6061 tgctgttggc gttgggggct ggggcgttaa ttgcgctgct gcgtacaccg attatcgatc
     6121 tggcgctgca tattgttggc ggtagtgaag cggtcctgga acaggcgcgg cgctttcttg
     6181 aaatccgctg gttaagcgct ccggcgtcgc tggcgaatct ggtattactc ggttggttac
     6241 tcggcgtgca atatgcccgt gcgccagtaa ttttgttagt ggtcggcaat atcctcaaca
     6301 ttgtgctgga tgtctggctg gtgatggggc tgcatatgaa cgtgcagggc gcggcgctgg
     6361 cgacggttat tgcggaatat gcaacattgc tgattggtct gctaatggtg cgtaaaatcc
     6421 tcaaactacg cggaatttcc ggcgaaatgc tgaaaactgc ctggcgagga aacttccgtc
     6481 gcttgctggc gcttaaccgc gatatcatgc tgcgttcgct gttgttgcaa ctctgtttcg
     6541 gcgcgatcac cgtacttggt gcgcgactgg ggagtgacat tgtcgctgtt aacgcggttc
     6601 tgatgacgct actcaccttt accgcctatg cgcttgatgg ttttgcctac gcggttgaag
     6661 cgcactccgg tcaggcatac ggtgcgcgcg acggtagcca gttgctggat gtatggcggg
     6721 cagcgtgccg ccagtcgggg attgtagcgt tactgttttc ggtggtttat ttgctggcag
     6781 gggaacacat cattgcgttg ctgacgtcgt taacccagat tcagcagctg gctgaccgct
     6841 atcttatctg gcaggtgatt ttgccgttgg ttggcgtctg gtgttatctg ctggacggca
     6901 tgtttatagg cgcaacgcgt gccgccgaaa tgcgtaacag tatggcggtg gccgccgcag
     6961 gttttgcgct gacgctcctt acgctgccgt ggctgggtaa tcatggtttg tggctggcat
     7021 taaccgtctt tctggcgttg cgcgggcttt ctctggcggc tatctggcgg cgtcactggc
     7081 gcaacggtac ctggtttgcc gcaacgtgac ggttaaaaat tctgaataaa taatcctaag
     7141 ccaaattgct gactacactt aatctcacgt tcagaagaaa agtgaacgta ctctcattca
     7201 caacctaacg atgaggtctt gattatgaat aaagatgaag ccggcggtaa ctggaaacag
     7261 tttaaaggta aagtgaaaga gcaatggggc aaactgaccg atgatgatat gacgatcatt
     7321 gaaggtaaac gtgatcaact ggtcggtaaa atccaggaac gttatggtta tcagaaagat
     7381 caggcagaaa aagaggtcgt ggattgggaa acccgcaatg aatatcgctg gtaattaatc
     7441 cctcctgccc gacgtgtaca aggatgtacg ccctcttaac gcggtttctt tttcacctgc
     7501 acagagtgat catgctggca ctgttcagga tgacgacacg cttccacttc cacgcatgcc
     7561 gcacagagcc catgtgcttc aatcacatta tgccgcaggg caaaccccat ttttgccgcc
     7621 agcgtatgca taatgtcttc cacgccttct gcacactctt ctttcaccgc gccgcagcga
     7681 tcgcaaataa acatggctga cgtatgggtg ggctggtcga acagatgaca gagcacataa
     7741 ctgttggtgg attccacctt atgcacaaaa ccttgctcaa gcagaaaatc cagcgcgcga
     7801 taaaccgttg gcggcttggc ttgcggttca gcttcgcgca gtaaatcaag cagatcataa
     7861 gcgctgatag cgccatcctg cagactcatc aggcgcaaca cttccaggcg ctgtggggtc
     7921 aggcgcacat tacgctgcgc gcagattttt tcagcctgcg ctaataactc ctgcgttgtg
     7981 gtcttttcca tcgggcacct caaagtgggg gttaagaatg tccttacttt accatgttcc
     8041 aggaaaaaca ccgagaaccc ttattgttgc cgtaatgttg attttctgtt ttgtaggtaa
     8101 ggtgttgtgt tgccttg
//
LOCUS       DAZGJW010000187         8087 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZGJW010000187 DAZGJW010000000
VERSION     DAZGJW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.187"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(304..1335)
                     /gene="rsmC"
                     /locus_tag="ACHKIK_004454"
     CDS             complement(304..1335)
                     /gene="rsmC"
                     /locus_tag="ACHKIK_004454"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO9085492.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVAKADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1438..1851
                     /gene="holD"
                     /locus_tag="ACHKIK_004455"
     CDS             1438..1851
                     /gene="holD"
                     /locus_tag="ACHKIK_004455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO9085493.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTDLRANPTARAALWQQICTYEHDFFPRND"
     gene            1820..2266
                     /gene="rimI"
                     /locus_tag="ACHKIK_004456"
     CDS             1820..2266
                     /gene="rimI"
                     /locus_tag="ACHKIK_004456"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO9085494.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2281..2958
                     /gene="yjjG"
                     /locus_tag="ACHKIK_004457"
     CDS             2281..2958
                     /gene="yjjG"
                     /locus_tag="ACHKIK_004457"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO9085495.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3049..4638
                     /gene="prfC"
                     /locus_tag="ACHKIK_004458"
     CDS             3049..4638
                     /gene="prfC"
                     /locus_tag="ACHKIK_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO9085496.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            5031..5636
                     /gene="osmY"
                     /locus_tag="ACHKIK_004459"
     CDS             5031..5636
                     /gene="osmY"
                     /locus_tag="ACHKIK_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO9085497.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5763..5924
                     /locus_tag="ACHKIK_004460"
     CDS             5763..5924
                     /locus_tag="ACHKIK_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO9085498.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6046..7119
                     /locus_tag="ACHKIK_004461"
     CDS             6046..7119
                     /locus_tag="ACHKIK_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO9085499.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7116..7898
                     /locus_tag="ACHKIK_004462"
     CDS             7116..7898
                     /locus_tag="ACHKIK_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO9085500.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         1923 a         2098 c         2194 g         1872 t
ORIGIN      
        1 cgcataccat caattcttaa aaagaattgc taccacggag gcgcattcta gtggttttca
       61 gcttttcgtc aatagttaat tatcgccgga ggtgtaattg ctggaaaaat gtccatcagg
      121 aaactaacgt gcaggttttg taggtcggat aaggcgttca cgccgcatcc gacacggtat
      181 ttgggtgcag atgccagatg cgacgcgggc gcgtcttatc tggcctacga agggctaacg
      241 tgcaggtttt gtaggttgta taaggcgagc acgccgcatc cgacaaggct tgcggcggga
      301 tacttaacct ttcttcgcct ggcgggtcat aatggcgcga tacaccttga agcgcccggt
      361 ttgcgcgata acttcatgga agccaaatgt ctcatccagc acgtccgggt aaggcaggaa
      421 ggcgttcgct acaattcgca gctcgccgcc gctattaaga tgacgcaccg cgccgcgaat
      481 cagcgtttgc gccgcatcca ggctggtttg catcccatcg tggaacggcg ggttggagat
      541 gatcatatca aaacgacctt tcacctcgga aaagacgttg ctggcaaaga cttcaccttc
      601 aacaccgttg gccgcaagcg tcgcacggct ggcttctacc gccggggcag agacatcgca
      661 caaggtgaga cgaattttcg gcgaatggcg cgcaaaggca actgaaagca cacccgcgcc
      721 acagccgaca tccagcactt tacctttcgt gtgcggggtt aacgtcgaga gcagcaactg
      781 gctaccgaca tccagaccgt cgcggctaaa cacgccaggc agcgttttga ccgtcaggcc
      841 atcgacgctg tattcgcccc agaatttatc cgcatcaaat actggctgtt tttccagacg
      901 accaaaatag aggccacagc gacgagcgct gtcgacttta ttcaacggcg cataatctgc
      961 cagcatctgc tcggcgctgc gcacgccgct gcggttctcg ccaacgacaa aaatatccgt
     1021 ccccaccggc agcagagaaa gtaaattcat caactggaac tgggcttccg gtttgttctt
     1081 cggccagtag taaatcagcg tatcgcaatc tgcgacgtca tccgccttgg cgaccagact
     1141 aaaacgggcg ttatccccca tctggcggct taatacctgc cagtggtgga attgctgggt
     1201 atgagcacgg ctggccgcgg tatctaaacg cgcgggcagg tcatcctgta agtctccggc
     1261 aaacagaata cggctttgtt cgaaatcatc actgtgacgc agcaagactt cacttgccgg
     1321 ggtaaatgca gacatggaat gctcctcaat tgatactggc ggcgattata gccatatgtt
     1381 ggcgcggtat cgacgaattt gctatatttg cgcccctgac aacaggagcg attcgctatg
     1441 acatcccgac gagactggca gttacagcaa ctgggcatta cccagtggtc gctgcgtcgc
     1501 cctggcgcgt tgcaggggga aattgccatt gcgatcccgg cacacgtccg tctggtgatg
     1561 gtggcaaacg atcttcccgc cctgactgat cctttagtga gcgatgttct gcgcgcatta
     1621 accgtcagcc ccgatcaagt gctgcaactg acgccagaaa aaatcgcgat gctgccgcaa
     1681 ggcagtcgct gcaacagctg gcggttgggt actgacgaac cgctatcact ggaaggcgct
     1741 caggtggcat caccggcgct caccgattta cgggcaaacc caacggcacg cgccgcgtta
     1801 tggcaacaaa tttgcacata tgaacacgat ttcttccctc gaaacgactg atttaccagc
     1861 ggcttaccac attgaacaac gcgcccacgc ctttccgtgg agtgaaaaaa cctttgccag
     1921 caaccagggc gagcgttatc tcaactttca gttaacgcaa aacggcaaaa tggcggcgtt
     1981 tgcgattacg caagtggtgc tggatgaagc tacattgttc aatattgcgg tcgatcctga
     2041 ctatcagcgt cagggattgg gaagggcgct gctggaacat ctgatcgacg aactggaaaa
     2101 acgcggcgtg gcgacgctct ggctggaagt ccgtgcctca aatgctgccg ccattgccct
     2161 gtacgaaagt ttaggcttta acgaggcgac gattcgccgc aattactacc ccaccacgga
     2221 cggtcgcgaa gacgccatca tcatggcgtt gccaatcagt atgtaagaca aggtggaata
     2281 atgaagtggg actggatttt ctttgatgcc gatgaaacgc tgtttacctt tgactcattc
     2341 accggcctgc agcggatgtt tcttgattac agcgtcacct ttaccgctga agattttcag
     2401 gactatcagg ccgttaacaa gccactgtgg gtggattatc aaaacggcgc gatcacttca
     2461 ttacagcttc agcacgggcg tttcgagagc tgggccgaac ggctgaaagt tgaagcaggc
     2521 ttgcttaacg atgcctttat taatgcgatg gcggaaatct gcacgccgct gccgggcgcg
     2581 gtttctctgc ttaacgccat tcgtggcaac gccaaaatcg gcatcatcac caacggcttt
     2641 agcgccttgc agcaagtgcg tctggaacgc acgggcctgc gtgattactt cgatttgctg
     2701 gtgatttccg aagaagttgg cgttgctaaa ccgaataaga aaattttcga ttatgcgctg
     2761 gaacaggcgg gcaatcctga ccgttcacgc gtgctgatgg ttggcgacac tgccgagtcc
     2821 gatattctcg gtggcatcaa cgccgggctt gcgacctgct ggctgaatgc gcacaatcgc
     2881 gagcaaccag aaggcatcgc gcccacctgg accgtttctt cgttgcacga actggagcag
     2941 ctcctgtgta aacactgatt gcctcccccc cgttgatggg taaaatagcc gcaatttttc
     3001 gttttcaaca agcgcggcgc gatgccgctt actcaagaag aaagaattat gacgttgtct
     3061 ccttatttgc aagaggtggc taagcgccgc acttttgcca ttatttctca cccggacgcc
     3121 ggtaagacta ccatcaccga gaaggtgctg ctgttcggac aggccattca gaccgccggt
     3181 acagtaaaag gccgtggttc caaccagcac gctaagtcgg actggatgga gatggaaaag
     3241 cagcgtggga tctccattac tacgtctgtg atgcagtttc cgtatcacga ttgcctggtt
     3301 aacctgctag acaccccggg gcacgaagac ttctcggaag atacctatcg taccctgacg
     3361 gcggtggact gctgcctgat ggttatcgac gccgcaaaag gtgttgaaga tcgtacccgt
     3421 aagctgatgg aagttacccg tctgcgcgac acgccgatcc tcacctttat gaacaaactt
     3481 gaccgtgata tccgcgaccc gatggaactg ctcgatgaag ttgagaacga gctgaaaatc
     3541 ggctgcgcac cgatcacctg gccgattggc tgcggcaagc tgtttaaagg cgtttaccac
     3601 ctttataaag acgaaaccta tctctatcag agcggtaaag gtcacaccat tcaggaagtc
     3661 cgcattgtta aagggctgaa taacccggat ctcgatgctg cggttggtga agatctggcg
     3721 cagcagctgc gtgacgaact ggaactggtg aaaggcgcgt ctaacgagtt cgacaaagaa
     3781 ttgttccttg cgggcgaaat cactccggta ttcttcggta ctgcgctggg taacttcggt
     3841 gtcgatcata tgctggatgg cctggtggag tgggctcctg cgccgatgcc gcgtcagact
     3901 gatacccgta ccgtagaagc gagcgaagac aaatttaccg gcttcgtatt taaaattcag
     3961 gccaacatgg acccgaaaca ccgcgaccgc gtggcgttta tgcgcgtggt atccggtaaa
     4021 tatgaaaaag gcatgaagct gcgccaggtg cgcaccgcga aagacgtggt gatctctgac
     4081 gcgctgacct ttatggcggg tgaccgttcg cacgttgaag aagcgtatcc tggcgatatc
     4141 ctcggcctgc acaaccacgg caccattcag atcggcgaca cctttaccca gggtgagatg
     4201 atgaagttca ccggtattcc gaacttcgca ccagaactgt tccgtcgtat ccgcctgaaa
     4261 gatccgctga agcaaaaaca gctgctcaaa gggctggtac agctttccga agagggcgcg
     4321 gtgcaggtgt tccgtccgat ctccaacaac gatctgatcg ttggtgcagt tggtgtgctg
     4381 cagtttgatg tagtggtatc gcgcctgaag agcgaataca acgttgaagc agtatatgaa
     4441 tcagtcaacg ttgccaccgc ccgctgggta gaatgtgcag acgcgaagaa attcgaagag
     4501 ttcaagcgta agaacgaaag ccaactggcg cttgatggcg gcgataacct cgcttacatc
     4561 gctaccagca tggtcaacct gcgcctggcg caggaacgtt atccggacgt tcagttccac
     4621 cagacccgcg agcattaatt cttgcctcca gggcgcggta gccgctgcgc cctgtcaatt
     4681 tcccttcctt attagccgct tacggaatgt tcttaaaaca ttcacttttg cttatgtttt
     4741 cgctgatatc ccgagcggtt tcaaaattgt gatctatatt taacaaagta atgacatttc
     4801 tgacggcgtt aaataccgtt caatgcgtag atatcagtat ctaaagccgt cgattgtcat
     4861 tctaccgata ttaataactg attcagaggc tgtaatggtc gttattcatc acccatcgct
     4921 tttgtgatgg cgaccattga cttctgtaga gggtgaagtc tctccctatt cagcaatgca
     4981 acctcgtgtt gccaggctca aattacgagc aaacatacag gaataaatcg atgactatga
     5041 caagactgaa gatttcgaaa actctgctgg ctgtaatgtt gacctctgcc gtcgcgaccg
     5101 gctctgccta cgcggaaaac aacgcacaga ctaccaatga aagcgcaggg caaaaagtcg
     5161 atagctctat gaataaagtc ggtaatttca tggatgacag cgccatcacc gcgaaagtga
     5221 aggcggcgct ggtggatcat gacaacatca agagcaccga tatctctgtc aaaaccgatc
     5281 aaaaagtcgt gaccctgagc ggtttcgttg aaagccaggc tcaggccgaa gaggcagtga
     5341 aagtggcgaa aggcgttgaa ggggtgacct ctgtcagcga caaactgcac gttcgcgacg
     5401 ctaaagaagg ctctgtgaag ggctacgcgg gtgacaccgc caccaccagt gaaatcaaag
     5461 ccaaactgct ggcggacgat atcgtccctt cccgtcatgt gaaagttgaa accaccgacg
     5521 gcgtggttca gctctccggt accgtcgatt cccaggcaca gagcgaccgt gctgaaagta
     5581 tcgccaaagc ggtagatggt gtgaaaagcg ttaaaaatga tctgaaaact aagtaattcg
     5641 tcgtaattcg tcctcccgaa ctttgtcggg aggcgcgatg tgcaccacac taaaaatatc
     5701 gcattgagtg attttcacgc tcatatcaag cggtgacatt aactatggta aaggagacgc
     5761 ttatgtttcg ttggggcatc atatttctgg ttatcgcgtt aatcgccgcc gcacttgggt
     5821 ttggtggtct ggccggtacc gctgcaggcg cagctaaaat tgtctttgtc gtcgggatta
     5881 ttctgttcct ggtgagtttg ttcatgggcc gaaaacgacc ctagatttca gaccattaag
     5941 aaaattttat ccaaagccag tccagcggac tggcttttgc ggttttagcg aataataaat
     6001 tgcgttactt tgtcattctt tcacaacaaa aacaggaagg cagaggtggg gcagcgaata
     6061 cctgtaacgc ttggtaatat tgcgccgttg tcgctaaggc cgttccagcc tggacgaata
     6121 gctctggtgt gcgaaggcgg cggacagcgt ggaattttca cggctggcgt gctggatgag
     6181 tttatgcgcg cgcagtttaa tcctttcgat ctttatctcg gcacatctgc cggggcgcag
     6241 aacctctcgg cgtttatctg caatcagccc ggttacgcgc gcaaagtcat catgcgctat
     6301 accacaaaac gcgaattttt cgatccattg cgctttgtcc gtggaggaaa tcttatcgat
     6361 ctcgactggc tggtggaggc caccgcaagc cagatgccgt tgcaaatgga taccgccgcg
     6421 cggttgtttg acagcggcaa atcgttttat atgtgcgcct gtcgtcagga tgactacgcg
     6481 cctaattact ttttaccaac caaacaaaac tggctggatg tgattcgcgc ctccagtgcg
     6541 atacctggct tttatcgtag cggagtgtcg ctggaaggca ttaactacct ggatggcggg
     6601 atcagtgatg cgattccggt taaagaggcg gcaaggcagg gcgctaaaac gttggtcgtc
     6661 atccgcactg tgccgtcaca aatgtactac acgccgcagt ggttcaaacg catggaacgc
     6721 tggctgggtg acagtagcct gcagccgctg gtcaatctgg tgcagcatca tgaaaccagc
     6781 tatcgtgaca ttcagcaatt tattgagaaa ccaccgggca agctgcggat attcgaaatt
     6841 tatccaccga aaccattaca tagtatcgcg cttggcagtc ggattccggc gctgcgtgaa
     6901 gactataaac ttgggcgttt atgcggtcgt tatttcctcg ccacggttgg caagctatta
     6961 actgaaaaag cgccgcttac ccgccatctg gtgccagtgg tgacgccgga atcgatcgtt
     7021 attccgcctg cgccagtcgc caacgatacg ctggttgccg aagtgagcga tgctccgcag
     7081 gcgaacgacc cgacatttaa caatgaggat ctggcttgat ttgccgtttt atcgataccc
     7141 actgccattt tgatttcccg ccgtttagtg gcgatgaaga ggccagcctg caacgcgcgg
     7201 cacaagcggg cgtaggcaag atcattgttc cggcgacaga ggcggaaaat tttgcccgcg
     7261 tgcaggcgtt agcggaaaaa tatcaaccgc tgtatgccgc attggggttg catccgggga
     7321 tgttggaaaa acatagcgat gtgtctcttg atcagctaca gcaggcgctg gaaaggcgtc
     7381 cggcgaaggt ggtggcggtg ggggagatcg gtctggatct cttcggcgac gatccgcaat
     7441 ttgagaggca gcagtggtta ctcgacgaac agctgaaact ggcgaaacgc tacgatctgc
     7501 cggtgatcct tcattcccgg cgtacacacg acaaactggc gatgcatctt aaacgtcacg
     7561 atttaccgtg cactggcgtg gttcacggtt tttccggcag cctgcaacag gccgagcggt
     7621 ttgtacagct gggctacaaa atcggcgtag gcggtactat cacctatcca cgcgccagta
     7681 aaacccgcga tgtcatcgca aaattaccgc tggcatcgtt gttgctggaa accgacgcgc
     7741 cggatatgcc gctcaacggc tttcaggggc agcctaaccg accggaacag gctgtgcggg
     7801 tgtttgacgt gctttgcgag ttgcgcccgg aaccagaaga tgagattgcc gaagtgttgc
     7861 ttaataatac gtatgcgttg tttagcgtta gtgggtagtt tacccttcgt aggcagcatc
     7921 cggcagtcgg cgcataatgc ctgatgcgac gcttgtcgcg tcttatcatg cctacaggtt
     7981 tgtgccgaac cgtaggccgg ataaggcgtt cacgccgcat ccggcagttg gcgcacaatg
     8041 cctgatgcga cgcttgtcgc gtcttatcat gcctacaggt ttgtgcc
//
LOCUS       DAZGJW010000188         8075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZGJW010000188 DAZGJW010000000
VERSION     DAZGJW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.188"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(17..238)
                     /gene="kilR"
                     /locus_tag="ACHKIK_004463"
     CDS             complement(17..238)
                     /gene="kilR"
                     /locus_tag="ACHKIK_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HFO9085501.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            complement(996..1121)
                     /locus_tag="ACHKIK_004464"
                     /pseudo
     CDS             complement(996..1121)
                     /locus_tag="ACHKIK_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415662.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1123..1278)
                     /locus_tag="ACHKIK_004465"
     CDS             complement(1123..1278)
                     /locus_tag="ACHKIK_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO9085502.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(1584..2003)
                     /locus_tag="ACHKIK_004466"
     CDS             complement(1584..2003)
                     /locus_tag="ACHKIK_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9085503.1"
                     /translation="MEKENEKIAASRLNDKIAMRLKERRQKLGLSQGKLAEICGWTQS
                     RIGNYEAGSRNVGVHDAVVLGKALGISPPELLFGEQESSELWLNESQRKLLELFNQLP
                     GSEQQRMIELFEVRLKEIDEYVEKYLRGRLKDNPSPE"
     gene            2100..2342
                     /locus_tag="ACHKIK_004467"
     CDS             2100..2342
                     /locus_tag="ACHKIK_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9085504.1"
                     /translation="MNKISTYRKQLGLSQRQLAVQLGWIQSRLANYEANFRTPGLEEC
                     RKIVSTLNRLGAHCGLDDVFPPDGKHSENSIGAVDS"
     gene            2339..2761
                     /gene="ydaT"
                     /locus_tag="ACHKIK_004468"
     CDS             2339..2761
                     /gene="ydaT"
                     /locus_tag="ACHKIK_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO9085505.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            3184..3969
                     /locus_tag="ACHKIK_004469"
     CDS             3184..3969
                     /locus_tag="ACHKIK_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085506.1"
                     /translation="MRELKRKGNAASVTQTDQKALPEEEEEEDINTDLTHPKPFPSGR
                     EFRDFVAGVLEGRLSGGTAAEFCNSAVVALQAAGLDVCREYPVPERGDGCGGRIDIVV
                     TDRNGVRCGIELDRNSPRQKSLLKIGAVETGICVLRRSDIARHTEQGILVIGGAVRQK
                     KFDPLSVDLPDWLPETLWHEWVQFRQALRKPIRTEQGANGAIRELEKFRQQGFTPEQV
                     IRHSIANEYQGLFAPKGVRPETLLRQVNTVSFPDSAIPPGFRG"
     gene            3976..4722
                     /gene="ydaV"
                     /locus_tag="ACHKIK_004470"
     CDS             3976..4722
                     /gene="ydaV"
                     /locus_tag="ACHKIK_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO9085507.1"
                     /translation="MKNIATGGVLERIRRLAPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGYT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGKTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCQVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVVQPGIAK"
     gene            4745..5515
                     /locus_tag="ACHKIK_004471"
     CDS             4745..5515
                     /locus_tag="ACHKIK_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO9085508.1"
                     /translation="METVFDALKAMGKATSVELAARLDISREEVLNELWELKRNGVVD
                     KTGHTWFLAVEGESGVTEGQALQPEAPDVVTEEVAPKVSADMMIEFIAQEGAKTCEEI
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGRFRYCMPGDNLPAEPKAALVTENDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            5531..5923
                     /locus_tag="ACHKIK_004472"
     CDS             5531..5923
                     /locus_tag="ACHKIK_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO9085509.1"
                     /translation="MAKVFTPEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSGCGVFLSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQN
                     IICRECRQSEVMQRVLAFYRGNFQEVME"
     gene            5920..6216
                     /locus_tag="ACHKIK_004473"
     CDS             5920..6216
                     /locus_tag="ACHKIK_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO9085510.1"
                     /translation="MRVRVYIAGPMTGYKNFNREAFNKAEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMSMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHTVIYQEVAQ"
     gene            6213..6986
                     /locus_tag="ACHKIK_004474"
     CDS             6213..6986
                     /locus_tag="ACHKIK_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO9085511.1"
                     /translation="MREVNYEALREAAEKATKGCYIVGHTSGNQHGNITGVFVCQKWK
                     GEPGGVIAECHVNCLVETDAQAYANAEFIAAFNPNVALALLDERERNQQYIKRRDQEN
                     EDIALTVGKLRVELEAAENNLIDSECHVAELEEALRDKQALLEASEKRNAKLQSENAY
                     IRNRYKELDLLIGKNILVMQAAIIEWQATGDAKSGLAWIYNTLFGPGELPDESEKDAQ
                     AYFNRKYAPIDEKLMALHKWFWEQSEAERAAGIRIKGGE"
     gene            7138..7326
                     /locus_tag="ACHKIK_004475"
     CDS             7138..7326
                     /locus_tag="ACHKIK_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085512.1"
                     /translation="MKNRKAKILLVRRNAPGVWQWVRLSNRRMGLMKYYGMMDCGFCK
                     KPSAEQNRWKNHLRTKGE"
     gene            7328..7537
                     /locus_tag="ACHKIK_004476"
     CDS             7328..7537
                     /locus_tag="ACHKIK_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085513.1"
                     /translation="MAIAASYTMHLYCDCLQCTDGKYKSPDFGEYIGTSWAGCAKEAR
                     KDGWRISKDKTRAFAPGHKVLRINK"
     gene            7534..>8075
                     /locus_tag="ACHKIK_004477"
     CDS             7534..>8075
                     /locus_tag="ACHKIK_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO9085514.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREIS
                     ETSTNYPVTPDGWISCSERMPDDGQHVIILCDGAFVLYAQYRDGEFFDVVRNGDEFFE
                     TQSRNVTDWMPLPEPPQEVR"
BASE COUNT         2162 a         1784 c         2181 g         1948 t
ORIGIN      
        1 tttattttct tagtcatcac catgactccg cctttacagg taaaccatca cgaccgagga
       61 agactttaat catgcagtca gaaatgcatg tttttgtagt caggttacga atataaagtt
      121 ttcgcttttt aatattgttt gccgaggcga tatatgtccg gccttcatga agaacataat
      181 caccaggagt cacacactga cgtggtattt catcagttcc gaagtgatgt gcaatcataa
      241 ttatctccat ttttacaaat gaactttgtt gatgcggtgc ctggtgcctc caggtgactg
      301 caaccagtta acaattacag tcggctttcc cacccaaacc aataaggact aacatgactt
      361 ttaactgtgc cgcgtgcgct tagccgcatt caccgcatca caaaattcac tttaaaaagg
      421 gcggacatca gtcgaacttc aagaaaaaac tgatgccgcc aagactacac acagcagtgt
      481 tgttattcac aaccggaggc gcactcccac catttaaatt taacagacaa gaccgactct
      541 ttatggatac cggaaatgcg ccttcgtgtt gtgcccggtt ttatttcacc acctccgggc
      601 tttggtggta tctttactgg agttctcaca caaccagtaa ggaaatgaat atgccaacgt
      661 atctcgccag agtagaacta tataatgctg agccagagga ttacgaaggg cttcataaat
      721 atatgctctc actaggattc aggagaacga tcccccatgg agatggttcg tataatcaac
      781 tcccggacgg aacttatgtt tccgaaaagg gcggtgatat ttatcaaatt cgcagccaga
      841 tatctgacta tgcagaccga ctatccagat atcgcgcgtc tgtttttgtt tgcgaattca
      901 gccaatgcgc atggtattta taccccgcca agacccggtg aatatcctgc acggccttct
      961 gctttgtaga aggcttcttc gctatcataa tcgccagatt ccagcgcttc tttagccagc
     1021 cagatgcgtg ccccagtgcc tgcatttggc tccagttgct ggaggcgttt tgcatcttct
     1081 gggagtagag cgataacgtg ttttaattct gtctcgttca ttttactcac ctgaatgtct
     1141 tcccaaccaa cgacgtgcgc cagcttcggt tttaaacgtt ttgcttttgg tatatgtcat
     1201 ggcggtgaat gtgccgtcct gattgggaaa cacgccacat accagagatt cgttgttgcc
     1261 aagatcgata gtatccatgt tgacctcatt tccccttaac gccgggtggc ggaacgtttt
     1321 atctactgcg ctttgtatca atcaacaact gccgtcatgt tcgtatgcct caggctggct
     1381 acttagccct gttcagtggc tggataactc gaggtattgt cctgccgttc tctggtgggg
     1441 cgttgtttgg atatgcttat taaacacaat acgttttctt atgtcaacac gaaatgtgtt
     1501 ttgtggtgag tgtcatatga tgatggtaca aaaaagcccg ctgatagcgg gctgattggc
     1561 atattactgt gatagcaaga tcattactcc ggtgagggat tatctttaag cctgcctctc
     1621 aaatattttt ctacatactc atcgatttct tttagccgga cttcaaatag ctcaatcatt
     1681 cgttgttgtt ctgagcccgg tagctggtta aacaactcaa gaagttttcg ttgggattca
     1741 tttaaccaca attcagaaga ttcctgttct ccaaagagga gctcaggagg agatatgcca
     1801 agtgcctttc ccaatacgac agcgtcatgc actccaacat ttctgctgcc cgcctcatag
     1861 ttacctatac gcgattgcgt ccatccgcag atttcagcaa gttttccttg agataaacca
     1921 agcttctgcc tgcgctcttt aagacgcatt gcaattttgt cattgagcct actagcggca
     1981 attttttcgt tttctttttc cattgcatcc ttgtatcacg aatcgtgatt tacataaaac
     2041 acaaaacagc ttgaccatat aacacaagat gtgtttaaaa ttgtcatcgg aggttttcaa
     2101 tgaacaaaat ttcaacatat cgaaaacagc ttgggctgtc tcaaagacaa cttgctgttc
     2161 agttagggtg gatacaaagc cgactggcaa attacgaagc aaattttcgt acccctggac
     2221 tagaggagtg cagaaaaatt gtttctaccc ttaatcggct tggcgctcat tgtggacttg
     2281 acgatgtatt ccccccagac ggtaagcata gcgaaaacag cataggagcg gttgattcat
     2341 gaaaatcagg catgagcaca tcgaatcagt gttgttagcc ttggcagccg aaaaagggca
     2401 ggcgtgggtc gctaacgcaa ttactgaaga atatctgcgc caggggggcg gcgaattgcc
     2461 ccttgtacca ggcaaggact ggaacaatca gcagaatatc tatcaccgtt ggttgaaagg
     2521 tgaaacgaaa gcgcaaaggg aaaaaattca gaaactgatc cctgcggttc tggcaattct
     2581 tccgcgcgag ctgcgtcacc gactctgcat cttcgatacc ctggaacgcc gtgcattact
     2641 ggcggcgcag gaagcgttga gtacggcaat tgatgcgcat gatgatgcag tccaggccgt
     2701 ttaccggaaa gcacatttca gcggtggtgg gtcgcccggc gattctgtcg tagtgcattg
     2761 attgaaatta atcgtgccgg attattttgt tcggtatcag ttaaatgtaa cgctgcgagc
     2821 gttacaaggt gaaaacaaat ggcttcaaac tggataaagc tcgaggttat tacgccggat
     2881 aagccggaaa tattcaggct tgctgagatt ctgaatattg atccagatgc cgcattaggg
     2941 aaggttattc gcttctgggc atgggcggat caacaaatga tagacggtaa cgcagattgt
     3001 aacgctcgcg gcgttacaaa aagtgcaata gatcgcatca cttttatggc tggttttgct
     3061 gatgcgttaa ttcaggttgg atggctggtc gaaaatgacg gtgggctttc tctacctaac
     3121 tttgaacgtc ataacgggaa aagctctaaa aaacgggcgg ttacaaacga gcgagtaaca
     3181 aaaatacgtg aactgaaacg aaaaggtaac gctgccagcg ttacacaaac ggatcaaaaa
     3241 gcgttaccag aggaagagga agaggaagat ataaatactg atctaaccca tcccaaaccc
     3301 ttcccttccg gaagggagtt ccgggatttt gtggctggag tgcttgaggg gagattatct
     3361 ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc tgctggcctg
     3421 gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gctgcggagg gcggattgat
     3481 atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg aaattctccg
     3541 cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt cttgcggcgc
     3601 agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc tgttcgccag
     3661 aaaaaatttg acccgttgtc tgttgatctg cccgactggt tgccagaaac actctggcat
     3721 gagtgggttc agttcaggca ggcattgcga aaaccgattc gaacggagca gggcgctaac
     3781 ggggcgatac gggagctgga aaaattccgc cagcagggtt ttacccctga gcaggtgatt
     3841 agacacagca tcgccaatga ataccagggt ttgttcgcgc cgaaaggcgt tcggcctgag
     3901 acgttgcttc gacaggttaa caccgtctcg tttccagaca gtgctatccc accaggcttc
     3961 agggggtaac gaagcatgaa aaatattgcg acaggcggcg ttctggaacg tatccgcaga
     4021 ctggccccgc cacatgtaac cgcgccattc aggacggtgg cggagtggcg cgagtggcaa
     4081 cttgcagaag gccagaaacg ttgtgaggag atcaaccgtc agaatcgtca gttgcgggtg
     4141 gaaaaaatcc tgaatcgctc cggcatccag ccgttgcacc gcaaatgctc gtttgcgaat
     4201 taccaggtgc agaacgacgg ccagcgatac gcgttgagtc aggcgaaatc tatcgctgat
     4261 gaactgatga ccgggtatac aaattttgcg ttcagcggaa aacctggtac cggaaaaaac
     4321 catctggcgg cggctatcgg gaatcgcctg ctgaaagatg gcaagacagt gattgtggtt
     4381 accgtggctg atgtcatgag tgctctacac gccagctatg acgacgggca atcaggcgaa
     4441 aaatttttgc gggaactgtg ccaagtggat ctgctggttc ttgatgaaat tggcattcag
     4501 cgcgagacaa aaaacgagca ggtggtgctg caccagattg ttgatcgccg gacagcgtcg
     4561 atgcgcagcg tggggatgct gacaaacctg aactatgagg ccatgaaaac attgctcggc
     4621 gagaggatta tggatcgcat gaccatgaac ggcgggcgat gggtgaattt taactgggag
     4681 agctggcgtc cgaatgtcgt ccagccagga attgcgaagt gatttttacc gggaggaaat
     4741 tttaatggaa accgtttttg atgcactgaa agcaatggga aaagccacgt cggttgaact
     4801 ggctgcgcga cttgatatca gtcgtgaaga agtgctgaac gagctgtggg aactcaaaag
     4861 aaatggcgtc gttgataaaa caggtcatac ctggtttctg gctgtcgaag gtgaatccgg
     4921 ggtaaccgaa gggcaggcac tacaacctga agcgccggat gtggtaaccg aagaggtcgc
     4981 tccaaaagtt agcgctgaca tgatgattga gtttatcgct caggaggggg ctaaaacctg
     5041 tgaagaaata gcgggtaagt tcggtgtcag tactcgcaag gttgcttcca cgctggcggt
     5101 ggtaaccgca acggggcggc tggcacgcgt taatcagaac ggtagatttc gttactgcat
     5161 gccgggcgat aatttaccag cagagccgaa agccgcgctg gtaacggaaa atgatggtaa
     5221 ggcctttcct cagccagcag gtgctgcgtt accagtccgg gaagccgcaa cacaggaaga
     5281 aattaaaaca gaaactgtgg cggacattgt gcagccgttg ccatcgttta ccgaaacgca
     5341 agcagatgag ctgatttttc cgtcccttcg cagggcaaac ctggcgctgc gcagggcgaa
     5401 aagtgatgtt cagaagtggg agcgagtctg cgccgcgctg cgggagctga acaagcaccg
     5461 ggatattgtt cgacagatta ctgattcttc ccgccgtgtt gtatcggaaa agtgattgcc
     5521 ggaggcgctt atggcaaaag tatttacacc agaagagcgg gaagaagtga aggcgcgcat
     5581 tgtggaattc gtgcgcctga gcggacgaga aacttttcga caactggcag ataaaacggg
     5641 tgtcagtaag accgctattc gtcgtttatc tggtgcgctt gcggccagtg gtgatgtctg
     5701 gctctctggt tgcggggtat ttctatcaga gcaggcgtat cgcgtatggc gcaagacacc
     5761 ggagaaggct gctgacccga cactgattcg aaagttacct gacggagaaa tacgtcgtta
     5821 caacagacgg cagaacataa tttgtcgtga gtgccgccag agcgaagtta tgcagcgtgt
     5881 gctggcgttc tatcggggta attttcagga ggtgatggag tgagggtgag ggtttatatc
     5941 gccggtccga tgaccgggta taaaaatttc aaccgtgagg cgttcaacaa ggcggaagag
     6001 gaactgaaac gggaagggca tacagtctta aacccggcag tacttccgga cgggctgaca
     6061 cagccgcact acatggatat ttgcatgtca atgatacgct gcgtggatgc gatttacatg
     6121 ctgaatggct ggcagcggtc agcgggcgct aaggcagagc tggcactggc ggagaaactg
     6181 ggacatacgg tgatttatca ggaggtggct caatgagaga ggttaactat gaggcgcttc
     6241 gtgaagcagc agagaaagca actaaaggat gctacatcgt agggcataca tcgggcaatc
     6301 agcatgggaa tataacagga gtttttgttt gtcaaaaatg gaaaggagaa cccggtggcg
     6361 taattgcaga atgtcatgtt aactgcctgg ttgaaacaga tgctcaggct tacgcaaacg
     6421 ctgaatttat tgctgccttt aatccaaatg ttgcgctggc actactggat gaacgggaaa
     6481 gaaaccagca atacatcaaa cgccgcgacc aggagaacga ggatattgcg ctaacggtag
     6541 ggaagctgcg tgttgagctt gaagcagcag agaacaacct tattgatagc gaatgccatg
     6601 ttgctgaact ggaagaagcg ctacgcgata agcaggcgtt acttgaagcc tcagaaaagc
     6661 gcaacgcaaa attacaaagc gagaatgcat acatccgcaa ccggtacaaa gaactggacc
     6721 tattaatcgg gaaaaacatt ctggtcatgc aggctgccat tatcgaatgg caggcaactg
     6781 gcgacgctaa gagcggacta gcatggattt ataacacact gtttggccct ggcgaattac
     6841 cggacgaatc tgagaaagat gctcaggcct actttaatcg caaatatgca ccgattgacg
     6901 aaaagcttat ggcgcttcac aagtggtttt gggaacaaag tgaagccgag cgcgccgctg
     6961 gcattcgcat caaaggaggt gagtaatgcg tgtggcatgt atcggcttgt taccgtatcc
     7021 gactcgtttt tgggcttctg cgctaattgc aaagaacatc atcccgacac caaagcgccg
     7081 ccataccggt attgcagcgg cacgacgagc agcaaagaga cgcaggagag caaaacgatg
     7141 aaaaaccgta aagcaaagat tctgttagtt cgtagaaacg ctcctggcgt ctggcagtgg
     7201 gtgagactca gcaaccgacg gatggggttg atgaaatatt acgggatgat ggattgtggt
     7261 ttttgcaaaa agcccagcgc ggagcaaaac cgctggaaaa accacttgcg cactaaagga
     7321 gagtgatatg gctattgctg caagttacac catgcatctc tattgtgatt gcctccagtg
     7381 tacagatggc aaatataagt cgccagactt cggtgagtat ataggtacgt catgggctgg
     7441 ctgtgcaaaa gaggcgcgca aggatggctg gcgaataagc aaagacaaaa cgcgtgcttt
     7501 tgcgcccggg cataaagttt tgaggattaa caaatgacca ctattaccaa agagcgactg
     7561 ctgacaatca agcagtggcg cgaaacatac ggacctggta gcaacgttgt actgccagca
     7621 gaagaagcgg aagaactggc acgaattgct ctggcatcgc tggaagcaga gccgatagca
     7681 atggtagtgc ctgatgaaat ggatttgctt acctgccatc tcgacggtgt aactaaaaca
     7741 tatgctgatg gctggaacgc ctgccgcgtc gccatgcttc aggccggaaa ctttcgggaa
     7801 aataagaatt cgtcaaccaa taattttcgg gaaatctcgg aaacgtcaac caactatccg
     7861 gtaactccgg atggttggat aagctgtagt gagcgaatgc cggatgatgg tcagcacgta
     7921 attattttat gtgatggcgc attcgttctt tatgcgcaat atcgagacgg tgagtttttt
     7981 gatgtagtcc gtaatggtga tgaatttttc gaaacacaga gccgcaatgt aaccgactgg
     8041 atgccgctac cagaaccgcc gcaggaggtg cgcca
//
LOCUS       DAZGJW010000189         8026 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZGJW010000189 DAZGJW010000000
VERSION     DAZGJW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.189"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            168..1325
                     /gene="acrE"
                     /locus_tag="ACHKIK_004478"
     CDS             168..1325
                     /gene="acrE"
                     /locus_tag="ACHKIK_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO9085515.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1337..4441
                     /gene="acrF"
                     /locus_tag="ACHKIK_004479"
     CDS             1337..4441
                     /gene="acrF"
                     /locus_tag="ACHKIK_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO9085516.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            4694..4915
                     /locus_tag="ACHKIK_004480"
     CDS             4694..4915
                     /locus_tag="ACHKIK_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085517.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            5346..6371
                     /locus_tag="ACHKIK_004481"
     CDS             5346..6371
                     /locus_tag="ACHKIK_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9085518.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            6439..6828
                     /locus_tag="ACHKIK_004482"
                     /pseudo
     CDS             6439..6828
                     /locus_tag="ACHKIK_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            6883..>8026
                     /locus_tag="ACHKIK_004483"
     CDS             join(6883..7171,7171..>8026)
                     /locus_tag="ACHKIK_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612098.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
                     /protein_id="HFO9085519.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTSAERQRLKELERENRELRRSNDILRQASAYFCEGGV
                     RPPLEKMMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDDWLK
                     REIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTIS
                     RKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSS
                     METTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGRLG"
     misc_feature    7164..7280
                     /locus_tag="ACHKIK_004483"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT         1945 a         1913 c         2119 g         2049 t
ORIGIN      
        1 attaatcttt ttttgtgttt atgtgccttg agacgccagt attcataact attccttaca
       61 tcgacgaatg ataatttgta ggatagcaaa ctgtattttt ctttctgcga gttaacgcgt
      121 tacctttctg ggtaaataac gcgcttttgg ttttttgagg aatagtaatg acgaaacatg
      181 ccaggttttt cctcctgccc tcctttattc tgatctccgc ggctttaatc gccggttgta
      241 acgataaggg agaagagaaa gctcacgtcg gtgaaccgca ggttaccgtt catattgtga
      301 aaacggcccc gttagaagtt aagactgaat taccaggccg caccaatgct taccgtatag
      361 ccgaagttcg cccacaggtt agcgggatcg tactgaatcg taatttcact gaaggcagcg
      421 atgtacaagc aggccagtcc ctgtaccaga tcgatcccgc gacctatcag gcaaattatg
      481 acagcgcgaa aggcgaactg gcgaaaagtg aagccgccgc cgccatcgcg catttgacgg
      541 taaaacgtta cgttccgctc gtgggtacga aatacatcag ccagcaggag tacgaccagg
      601 ccattgctga tgctcgccag gccgatgccg ccgtgattgc cgcaaaagcc acagtcgaaa
      661 gcgctcgcat caatctcgct tataccaaag tgactgcgcc aattagcgga cgtatcggca
      721 aatcgaccgt gaccgaaggc gctcttgtca ctaatgggca aacgactgaa ctggcgactg
      781 tccagcagct cgatcctatc tacgttgatg tgacccaatc cagcaacgat tttatgaggc
      841 tgaagcaatc cgtagagcaa ggaaatttgc ataaggaaaa cgccaccagc aacgtagagt
      901 tggtcatgga aaacggtcaa acctatcccc tgaaaggtac gctgcaattc tctgatgtga
      961 ccgttgatga aagcaccggc tccataaccc tacgtgctgt cttccctaac ccgcaacata
     1021 cgcttttgcc gggtatgttt gtgcgtgcac ggattgatga aggcgtccaa cctgacgcca
     1081 ttcttatccc gcaacaaggc gttagccgca caccgcgtgg tgatgcaacc gtgctgattg
     1141 ttaacgataa aagtcaggtg gaagcgcgcc ctgtcgttgc cagtcaggcg attggcgata
     1201 aatggttgat tagcgaaggt ctgaaatctg gcgatcaagt cattgtcagc ggcctgcaaa
     1261 aagcgcgtcc gggagagcag gttaaagcca ctaccgatac ccccgcagat actgcatcga
     1321 agtaaggtaa tctgacatgg caaacttttt tattcgacga ccgatatttg catgggtgct
     1381 ggccattatt ctgatgatgg cgggcgcact ggcgatccta caattgcccg tcgctcagta
     1441 tccaaccatt gcaccgcctg cggtttctgt ttcggcaaac tatccaggtg ctgatgcgca
     1501 gaccgtgcag gatacggtga cgcaggttat cgaacagaat atgaatggta tcgataacct
     1561 gatgtatatg tcctccacca gcgattccgc cggtagcgtg acaattaccc ttaccttcca
     1621 gtccgggacc gatcctgata tcgcgcaagt gcaggtgcag aacaaactcc agctcgccac
     1681 gccgttgctg ccgcaggagg ttcagcagca ggggatcagt gttgaaaagt ccagtagcag
     1741 ctatttgatg gtggcgggct ttgtctctga taacccagac accacacagg acgatatctc
     1801 ggactatgtg gcctctaacg ttaaagatac gcttagccgt ctgaatggcg tcggtgacgt
     1861 acagcttttc ggcgcacagt atgcgatgcg tatctggctg gacgccgatc tgctaaacaa
     1921 atataaactg acaccggttg atgtgattaa ccagttgaag gtacagaacg atcagatcgc
     1981 tgccggacag ttgggcggaa cgccagcgtt accagggcaa cagttgaatg cctcgattat
     2041 tgctcaaacg cggttaaaaa atccggaaga attcggcaaa gtgaccctgc gcgtaaacag
     2101 tgacggctcg gtggtacgcc tgaaagatgt cgcacgggtt gaacttggcg gtgaaaacta
     2161 taacgttatc gctcgtatca acggaaaacc ggcggcgggc ctggggatta agctggcaac
     2221 cggcgcgaat gctctcgata ccgcgaaagc cattaaggca aaactggcgg aattacagcc
     2281 attcttcccg cagggaatga aggttctcta cccttatgac accacgccat tcgtccagct
     2341 ttctattcac gaagtggtaa aaacgctgtt cgaagccatt atgctggtgt tcctggtgat
     2401 gtatctgttc ttgcagaata tgcgagcaac gctgatcccc accattgcgg tacccgtggt
     2461 gttgttaggg acgtttgcca tcctcgccgc ttttggttac tccatcaaca cactaacgat
     2521 gttcgggatg gtgcttgcca tcgggctgct cgtcgatgat gcgatagtgg tggtggagaa
     2581 cgtcgagcgc gtgatgatgg aggataagct cccgccaaaa gaagcgacgg aaaaatcaat
     2641 gtcgcaaatc cagggcgcac tggtgggtat cgcgatggtg ctgtcagcgg tatttattcc
     2701 gatggcattc ttcggcggtt ctactggggc aatttatcgc cagttctcta tcaccatcgt
     2761 ttcggcaatg gcgctttctg ttctggtggc attgattctt acccctgcgt tatgtgcaac
     2821 gctgcttaaa cccgtctctg ctgagcatca cgaaaataag ggcggtttct tcggctggtt
     2881 taatactacc ttcgatcata gcgttaacca ctacaccaac agcgtcggca aaatcctcgg
     2941 ttccacagga cgatatttac tgatctatgc gctgattgtt gcaggaatgg tggtgttgtt
     3001 tttacgtctt ccgtcttcct tcttacctga agaggatcag ggtgtctttc tgaccatgat
     3061 tcagttaccc gctggcgcga cgcaagagcg gacgcaaaaa gtgttggatc aagtgacgga
     3121 ttactatctg aagaacgaga aagcgaacgt tgaaagtgtc tttacggtta acggctttag
     3181 cttcagcggc caggcacaaa acgccggtat ggccttcgtc agtctgaaac cgtgggaaga
     3241 gcgtaatggt gatgaaaaca gtgcggaagc ggtaatccat cgtgccaaaa tggaatttgg
     3301 caagatccgc gacggttttg tcattccatt caatatgcca gccattgttg aactgggcac
     3361 ggcaacgggt ttcgactttg agttaattga tcaggctggg ctgggtcacg atgccctaac
     3421 ccaggcccgt aaccagttgc ttggtatggc ggcgcaacat cctgccagct tagtcagcgt
     3481 gcgtcctaat ggcctggaag acaccgcgca gtttaaactg gaagttgacc aggaaaaggc
     3541 gcaggcatta ggtgtttcac tttctgacat caatcagacc atttcaacgg cgttgggtgg
     3601 gacttacgtt aacgacttca tcgaccgtgg tcgcgtgaaa aaggtgtatg ttcaggcgga
     3661 tgccaaattc cgtatgctgc cagaagatgt cgataaactt tatgtccgca gcgccaacgg
     3721 cgaaatggtg ccattctcgg cctttaccac ttcacattgg gtgtatggct ctccgcgact
     3781 ggaacgctac aacggtctgc cgtcaatgga gattcagggg gaagccgcgc caggaaccag
     3841 ttccggcgat gccatggcgt tgatggaaaa ccttgcgtca aaattacctg ctggcattgg
     3901 ttatgactgg acgggtatgt cgtatcagga acgcttatcg ggaaaccagg ctcccgctct
     3961 ggtagcaatt tcctttgtgg ttgttttcct gtgccttgct gcactctatg aaagctggtc
     4021 aattcctgtc tcggttatgt tggtcgtgcc gttagggatt gtcggcgtgc tgctggcggc
     4081 gacactcttt aatcaaaaaa atgacgtcta ctttatggtg ggcttgctaa cgacaattgg
     4141 cttgtcggcc aaaaacgcta ttttgattgt tgagttcgct aaagatctca tggagaaaga
     4201 gggtaaaggt gttgttgaag cgacactaat ggcagtacgt atgcgtctgc gtcctatcct
     4261 gatgacctct ctcgccttta ttctcggcgt attaccgcta gctatcagta acggtgccgg
     4321 cagtggcgcg cagaacgcag tgggtatcgg ggtaatgggc ggaatggtct ctgcaacgtt
     4381 gctggcaatc ttcttcgtac cggtgttctt tgtggtgatc cgccgttgct ttaaaggata
     4441 aatcagaaac ataaaggcac tttcgggtgc ctttattatt ttcagtgaaa cccataaaaa
     4501 ttaataagat attcttccac tcactcttta aaaactttct atagttcccg ctcccttcac
     4561 tatttttaca attcacataa tttgtcatta ttcattccgc aatatttata acctccggtg
     4621 ataaaatggc attcagctcg ttaataagag agttaactta ttaagcgtta gcgtttatta
     4681 ctgaggtaac accatgaaaa gattaattcc tgtggcattg ctcaccgcat tgctggcggg
     4741 ctgtgctcac gattcgccct gcgttccggt ttatgacgat cagggtcgtc tggttcacac
     4801 caatacctgt atgaaaggca cgactcagga taactgggaa acggctgggg ctattgccgg
     4861 cggggcagcg gctgttgctg gcctgacgat ggggatcatc gctctgtcga aataactgaa
     4921 cagggcgcgg agtttcgcgc tctgtttaac gatacagtat ctttacataa gataaaatat
     4981 tgctatattc tgctggctaa ttctctgctg atttagcatg ttatatcgcg ttatttcgct
     5041 gctttcgaaa taacgcatca atttaacttt cacacttccc accagaaata tactcacgct
     5101 aacaatcatt attctgctta gtaaaacacg cgtaaatatt ttgcgccaaa atgtggcgca
     5161 tgtttcattt tcgcaccatt gcggggcgct gtttttatta tttcctgtct acactcagct
     5221 tattgcgacg cgaaatcggg cgcaaaagct ggcactactt ttgcttataa gaggatggcc
     5281 acagacaggt aaaagacgtt tcccaaacgt cctataacga taattttcgc ctcacaggaa
     5341 gcattatgaa aaagatgatg atagccacac tggctgccgc cagcgtgctg cttgccgttg
     5401 caaatcaggc gcatgctgga gcgacgcttg atgccgttca gaaaaaaggt tttgtgcaat
     5461 gcgggatcag tgatggatta cctgggttct cttatgccga tgcagacggt aagttttcag
     5521 gtattgatgt tgatgtttgt cgtggtgttg ccgctgctgt atttggtgac gacacgaaag
     5581 tgaaatatac cccactcaca gcaaaagaac gcttcacggc tttacagtca ggggaagtgg
     5641 atttgctctc ccgtaatacg acctggactt catctcgcga cgccgggatg ggaatggcat
     5701 ttactggcgt cacttattac gacggcattg gcttcctgac gcacgataaa gcggggctaa
     5761 aaagcgcgaa agaactggat ggcgctaccg tctgtattca ggcgggtact gataccgaac
     5821 tcaacgttgc cgactatttc aaggcaaaca atatgaagta cacaccggtg actttcgatc
     5881 gctctgacga atcagcgaag gcactggaat ctggtcgctg cgatacgctg gcctcggatc
     5941 agtcgcaact gtatgccctg cgcatcaaat taagcaaccc agctgaatgg attgtcttac
     6001 cggaggttat ctctaaagaa ccgcttgggc cggtagttcg tcgtggcgat gatgaatggt
     6061 tctcgattgt acgctggacg cttttcgcca tgctgaatgc tgaagagatg ggcatcaatt
     6121 cccagaacgt cgatgaaaaa gcggctaatc cagcaacgcc tgatatggca catctgctgg
     6181 gtaaagaagg cgattacggc aaggatctga agttggataa taaatgggcc tataacatca
     6241 tcaaacaggt gggtaactac tcggaaattt tcgagcgtaa tgtaggttca gaaagcccgc
     6301 tgaaaattaa acgtgggcaa aataatctct ggaataacgg cggtattcag tacgcgccgc
     6361 ccgtgcgtta agtcggtgtg aggtaacgag cgtcgctacg gcggcgctct cttcagcgtt
     6421 atgctttccg aggtttctat gtctcatcgc cgctcaaccg ttaaaggctc actctccttt
     6481 gccaacccta cggttcgtgc ctggttattc cagatccttg ccgttgttgc tgttgtcggc
     6541 attgttggtt ggttatttca caacactgta acgaatctca gtaatcgtgg cattacttca
     6601 ggttttgcct ttctggatcg cggcgctggc ttcggtattg tccagcattt gatcgattac
     6661 cagcagggcg acacctacgg acgcgttttt attgtcggct tactcaatac gctactggtt
     6721 tctgcattgt gtattgtgtt cgcttctgtt ttgggcttct ttatcggtct ggcgagactt
     6781 tcggataact ggctgctacg aaagctttcc acaatttata ttgagatctg aaccgccccg
     6841 gaaatcctgg agactaaact ccctgagaaa gaggtaaaca ggatgactaa aaatactcgt
     6901 ttttcccccg aagtccgtca gcgggcgatt cgtatggttc tggaaagtca ggatgaatat
     6961 gactcacagt gggcggcaat ttgttccatt gccccaaaga ttggctgtac gccggagacc
     7021 ctgcgtgtct gggttcgcca gcatgagcgg gataccgggg gcggtgatgg tgggctcacc
     7081 agcgctgaac gtcagcgtct gaaagagctg gaacgtgaaa atcgtgaact gcgccgcagt
     7141 aacgatatcc ttcgccaggc ttccgcttat tttgcgaagg cggagttcga ccgcctctgg
     7201 aaaaaatgat gccactgctg gataagctgc gtgagcagta cggggtcgga ccggtatgca
     7261 gcgaactgca tattgccccg tcaacgtatt accattgtca gcaacagcga catcatccgg
     7321 ataaacgcag tgcccgtgcg cagcacgacg actggctgaa gagagagata cagcgcgtat
     7381 acgatgaaaa tcatcaggtg tacggtgtgc gtaaagtctg gcgtcagttg ttacgggaag
     7441 gaatcagggt ggccagatgt acagtggcac gtctcatggc ggttatggga cttgccggtg
     7501 ttctccgggg taaaaaggtc cgtacgacca tcagccggaa agccgttgcc gcaggcgacc
     7561 gcgtaaaccg tcagttcgtg gcagaacgac ctgaccagct gtgggtggct gattttactt
     7621 acgtcagcac atggcagggc ttcgtctatg tggcgtttat cattgatgtg tttgccggat
     7681 acatcgtggg gtggcgggtc tcatcgtcta tggaaacgac attcgtgctg gatgcgctgg
     7741 agcaggcgtt gtgggcccgt cgtccgtctg gcaccatcca tcacagcgat aaaggctctc
     7801 agtatgtgtc actggcctat acggagcgac taaaagaagc cggattactg gcatcaacag
     7861 ggagtacagg cgactcgtat gacaacgcga tggctgagag catcaatggt ctttacaaag
     7921 cggaggtaat acaccgtaag agctggaaaa accgtgcaga agtggaactg gccacactaa
     7981 cgtgggtgga ctggtataac aatcgacgat tgctgggaag gctggg
//
LOCUS       DAZGJW010000190         7953 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZGJW010000190 DAZGJW010000000
VERSION     DAZGJW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.190"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            149..760
                     /gene="ruvA"
                     /locus_tag="ACHKIK_004484"
     CDS             149..760
                     /gene="ruvA"
                     /locus_tag="ACHKIK_004484"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO9085520.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPTTDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            769..1779
                     /gene="ruvB"
                     /locus_tag="ACHKIK_004485"
     CDS             769..1779
                     /gene="ruvB"
                     /locus_tag="ACHKIK_004485"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO9085521.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1926..2711)
                     /gene="znuB"
                     /locus_tag="ACHKIK_004486"
     CDS             complement(1926..2711)
                     /gene="znuB"
                     /locus_tag="ACHKIK_004486"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO9085522.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(2708..3463)
                     /gene="znuC"
                     /locus_tag="ACHKIK_004487"
     CDS             complement(2708..3463)
                     /gene="znuC"
                     /locus_tag="ACHKIK_004487"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO9085523.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3542..4474
                     /gene="znuA"
                     /locus_tag="ACHKIK_004488"
     CDS             3542..4474
                     /gene="znuA"
                     /locus_tag="ACHKIK_004488"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO9085524.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4490..5812
                     /gene="mepM"
                     /locus_tag="ACHKIK_004489"
     CDS             4490..5812
                     /gene="mepM"
                     /locus_tag="ACHKIK_004489"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO9085525.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQATQEDEAIPLDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5932..6903
                     /gene="lpxM"
                     /locus_tag="ACHKIK_004490"
                     /gene_synonym="msbB"
     CDS             5932..6903
                     /gene="lpxM"
                     /locus_tag="ACHKIK_004490"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO9085526.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(6992..>7953)
                     /locus_tag="ACHKIK_004491"
     CDS             complement(6992..>7953)
                     /locus_tag="ACHKIK_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HFO9085527.1"
                     /translation="EIRAYHEDAGFFAAWDFASNYRDGESYVLLAEKMTTVISGPKTT
                     MTLIYHYNSSYLRFAFTLTQDSPRVDVQTFIDWHEPNVSLKVKFPVSVQTSLAQCQIQ
                     FGVIDRPTHSDDSFAFAKDEIPAHHWVDLSDQETGIALIAKNKYGYRVKDQTLELTLL
                     RSQHKPGEVVKTDNYDNFLINNFADIGKQKFTFSLFPHHGDYRVGGVVNQAYIMNHPL
                     QIVPVKPHPGELPPSLSFFAIDTNSVIIETIKLAEDGDGIIVRLYESYGLEISAMFSW
                     VCNYHYVQYVDLQENKLDDSFYCNQYCNLEFKPFEIITLRLTQ"
BASE COUNT         2110 a         1898 c         2021 g         1924 t
ORIGIN      
        1 tccaatataa cctgtagaat aaattatact gcgccatttt tcagatcgtt gggacgcctc
       61 gcaagttttc ttcatccttc gctggatatc tatccagcat ttttttatca tacagcatta
      121 tctttgattc attacgcagg agcgtcatgt gataggcaga ctcagaggca tcatcattga
      181 aaaacaaccc ccgctggtgt taattgaagt gggcggcgta ggctatgaag tgcatatgcc
      241 gatgacctgt ttttatgaac tccctgaagc gggtcaggaa gcgatcgttt tcacccactt
      301 tgtggtgcgt gaagacgcgc aactgctgta cggttttaac aataaacaag agcgcacatt
      361 gttcaaagag ttgatcaaaa ccaacggcgt cggcccgaag ttggcgctgg cgatcctctc
      421 cggaatgtca gcgcagcagt tcgttaatgc cgttgagcgt gaagaagtgg gggcactggt
      481 gaaactgccg ggtattggca aaaaaaccgc cgaacgcttg attgttgaaa tgaaagaccg
      541 atttaaaggt ttgcatggcg atctctttac gccagccgcc gacctggtac tcacgtcacc
      601 tgccagcccg acgaccgacg atgctgaaca ggaagcggtt gccgcgctgg tggcgctggg
      661 ctataaacca caagaagcaa gccgcatggt gagcaaaatc gctcgccctg acgccagcag
      721 tgaaacatta attcgcgaag ccctacgcgc cgcgttatga ggtaaaggat gattgaagca
      781 gaccgtctga tttctgccgg taccactttg ccggaagatg tggcagatcg cgccattcgc
      841 cctaaattac tggaagagta tgttggtcag ccgcaggttc gttcacagat ggagattttc
      901 atcaaagcag cgaaactgcg cggcgatgcc ctcgatcatc tgttgatttt cggtcctccg
      961 gggttgggta aaactacgct tgccaacatt gtcgccaatg aaatgggcgt taatttacgc
     1021 acgacttctg gtccggtgct ggaaaaggcg ggcgatctgg cagcgatgct cactaacctt
     1081 gaaccgcatg acgtgttgtt tattgatgag atccaccgtc tttcgccagt ggtggaagag
     1141 gtgttgtatc cggcaatgga agactaccaa ctggatatca tgattggtga aggtccggcg
     1201 gcacgctcca ttaaaattga tttgccgccg tttaccctga ttggtgcaac cacgcgcgca
     1261 ggttcgctga catcaccgtt gcgcgatcgt tttggtattg tgcagcgtct ggagttttat
     1321 caggtgccgg atctgcaata tatcgtcagt cgcagcgcac gctttatggg gcttgagatg
     1381 agtgatgacg gcgcgctgga agttgctcgt cgagctcgcg gtacgccgcg cattgccaac
     1441 cgtctgctgc gtcgagtgcg tgatttcgcc gaagtgaagc acgatggcac catctcggca
     1501 gatatcgctg ctcaggcgtt ggatatgttg aatgtcgatg ctgaaggttt cgattatatg
     1561 gaccgcaagt tattgctggc ggtaatcgat aagttctttg gtgggccggt aggtctggat
     1621 aaccttgcgg cagccattgg cgaagaacgt gaaaccattg aggatgtgct ggaaccttat
     1681 ttgattcagc aaggcttttt gcagcgtaca ccgcgtgggc gtatggcgac gacgcgggcg
     1741 tggaatcact ttggcataac gccgccagaa atgccgtaag tcggattggt cgctcgcgcc
     1801 gcatccgaca ctggcagtgt gtctgatgcg acactgttcg cgtcttatca ggccagccag
     1861 agatagcgaa ccgtaggtcg gataaggcgc tcgcgccgca tccgacaaat gtgttcagcg
     1921 atagattagc tggcctgctt tttcatcata ctgagaataa ataacagtgc cgcacatagc
     1981 accaccgacg gacccgccgg cgtatcgtaa aacgcggaaa aggttaaacc gccagttact
     2041 gccaccatcc ccaccaaaac agcgacacca gccatctgtt ccggcgtacg ggcaaagcga
     2101 cgcgcagtag ctgcaggaat aatcagcagt gaagtaataa tcaacgcgcc aacaaatttc
     2161 atcgctacac caatcgtcaa tgccgtcacc agcatcaaca acaatttcac gcgctgtaat
     2221 ttcacaccat caacaaacgc cagatccggg ctaatcgtca tcgacagcaa attgcgccat
     2281 tgccagaaca aaatagccac cacgatgacc acgccaatcg caatagcgat gagatcttct
     2341 ggcgtcactg ccagcaaatc accgaacagg taagccatca aatcaacacg aatgttagac
     2401 atcagactaa cgaccaccag gcccagcgac agggcactgt gcgccataat ccctaataac
     2461 gtgtcaatcg ccagctgtgg acgcttctcc agccatacca gaccgcccgc caggagcagc
     2521 gtaacggcaa ttaccgcata gaatggattc acgtccagca acaaaccaaa cgcgacgcca
     2581 agtaatgagg catgagccag cgtatcaccg aaataagaca tacgacgcca gactacaaac
     2641 gaacccagcg gacccgcggc acaggcgagc atgatcccgg ctaaccaacc gggaaataat
     2701 aattcaatca tgagcgatca tttccccgac gcaaaacaat tcgtccctgt aaatcgtgac
     2761 gatgattatg atgatggcga tagataccca gttgttcagc accacgagga ccaaacatag
     2821 aaataaactc cggatgtagg gaaacaactt ccggtgtgcc ggaacaacaa atgtggtgat
     2881 tcagacacag cacttcatcg gtttttgcca ttaccagatg cagatcgtga gagaccatta
     2941 aaacgccaca atccagttcg cgacgcaact ggtcaataag gtcatataac gccacctggc
     3001 catttacatc cacgccttga gtgggttcat ccagcactaa taattgcggt cgatttaaca
     3061 atgctcgcgc taacagtaca cgctgtgttt cgccaccaga gagcttttgc atcggtgcgt
     3121 taatcagatg cccggcctgg acacgtttca gtgcaggcaa aatatcttct ttatgtgtgc
     3181 cagggcgtaa gcgtaaaaaa cggtttacgg tcagtggcaa cgtggtgtcg agatacagct
     3241 tctgcggtac atagccgatg cgtagttttc cgttgcgctt gataacccct tcatcgggtg
     3301 ttaccagccc gagcactacc cgtaccagtg tcgacttacc tgcgccattt ggcccaagta
     3361 aagtcaaaat ttttccaggt ttaagttcca gcgacacatc agagaggacg cggcgttggc
     3421 caaaagaaac cgagacattt tccagggaaa ccagacttgt catgttaatt ttagtcttgc
     3481 agtagtcatg aaatgttata atatcacact tctcatattc attacgatga ttggtcgcat
     3541 tatgttacat aaaaaaacgc ttcttttcgc agcattatcc gccgctctct ggggaggtgc
     3601 aacacaggcc gcagatgccg ccgttgtcgc ttcgcttaaa cccgttgggt tcatcgcttc
     3661 tgccattgct gatggtgtaa cagaaacgga ggttttactg cctgacggcg cttcagaaca
     3721 tgattattca ctgcgtccat cggatgtaaa acgcttacag aacgcggact tagtcgtttg
     3781 ggttggcccg gagatggaag cgtttatgca aaaaccggta agcaaattac caggagcgaa
     3841 gcaggtaacg attgcgcagc ttgaagatgt gaaaccgcta ctgatgaaaa gtattcacgg
     3901 cgatgatgat gatcacgacc acgcggaaaa aagtgacgaa gatcaccatc acggcgattt
     3961 caacatgcat ctttggcttt ccccagagat agcgcgggct acagcggttg caatccatgg
     4021 aaaattagtg gaacttatgc cgcaaagtcg agccaaactt gacgccaacc tgaaggattt
     4081 tgaggcacaa ttagcctcaa ccgaaacgca ggttggtaac gagctcgcgc cgctcaaggg
     4141 gaaaggttat ttcgtttttc acgatgctta cggctatttc gaaaaacagt ttggactgac
     4201 accgcttggt cattttaccg ttaaccctga gattcaacct ggcgcgcagc gtttacatga
     4261 aataagaaca cagttggttg agcaaaaagc aacctgcgtt tttgctgagc cacagttcag
     4321 gccagcggtc gttgagagcg tcgcacgagg gacatccgtt cgtatgggaa cgttggatcc
     4381 cctggggacg aatatcaaac tgggtaaaac aagctattca gaattcctga gtcaattagc
     4441 caaccagtat gcgagctgcc tgaaaggaga ttaatgagga agtgattacg tgcaacagat
     4501 agcccgctct gtcgccctgg cgtttaataa tttaccgcga ccacaccgcg ttatgttggg
     4561 gtcgctcacc gttcttactc tggccgtcgc tgtctggcgg ccttatgttt atcaccgtga
     4621 tgccacgcca attgtcaaaa ccattgagct ggaacagaac gaaattcgtt cgctcttacc
     4681 tgaagccagt gagccgattg atcaagctac acaagaagat gaagccattc ccctggacga
     4741 actggatgac aaaatcgccg gtgaagcggg cgtgcatgaa tatgttgttt ccactggcga
     4801 tacgctaagc agcattctca atcagtatgg tattgatatg ggtgatatca cccaactggc
     4861 tgcggccgac aaagaattgc gtaacctgaa aatcggtcaa caactctcct ggacattaac
     4921 cgcggacggc gaactgcagc gcctcacctg ggaagtgtct cgtcgtgaaa cccgaaccta
     4981 tgaccgtact gccgctaacg gttttaaaat gaccagcgaa atgcagcaag gagagtgggt
     5041 taacaatctg ctgaaaggta ccgtcggggg gagctttgtt gccagcgcca gaaacgccgg
     5101 tttaaccagc gccgaagtga gcgcagtgat taaagccatg cagtggcaaa tggatttccg
     5161 caaactgaaa aaaggcgatg aatttgcggt gttaatgtct cgagaaatgc ttgatggtaa
     5221 acgtgagcaa agccagctgc tgggcgtacg tttgcgttca gaaggtaaag attattacgc
     5281 tatccgcgct gaagatggca aattctacga tcgtaacggt actggtctgg cgaaaggatt
     5341 cttgcgattc ccgacggcga aacagttccg tatctcgtct aactttaacc cgcgtcgaac
     5401 taatccggtg acaggtcgcg ttgcaccaca cagaggtgtt gatttcgcca tgccacaagg
     5461 tacgccagtg ctttcagtgg gtgacggtga agtggtggtt gccaaacgca gtggcgcagc
     5521 aggttattat gtggctattc gtcatggtcg cagctacacc acgcgttata tgcacttgcg
     5581 caagattctg gtgaaaccgg gacagaaggt gaaacgtggc gaccgtatcg cgctttccgg
     5641 taataccgga cgttcaactg ggccgcatct gcactatgaa gtatggataa accagcaggc
     5701 cgtaaacccg ctgacggcaa aactgccgcg tactgaaggg ctgaccggct ccgatcgtcg
     5761 cgaattcctg gcgcaggcca aagagattgt gccgcagcta cggtttgatt aattaacatc
     5821 cattcgcagc cggtacgcag tcagtaccgg ctttttttat ttggtgcggg gcaagttgcg
     5881 ccgctacact atcaccagat tgatttttgc cttatccgaa actggaaaag catggaaacg
     5941 aaaaaaaata atagcgaata cattcctgag tttgataaat cctttcgcca cccgcgctac
     6001 tggggagcat ggctgggtgt agcagcgatg gcgggtatcg ctttaacgcc gccaaagttc
     6061 cgtgatccca ttctggcacg gctgggacgt tttgccggac gactgggaaa aagctcacgc
     6121 cgtcgtgcgt taatcaatct gtcgctctgc tttccagaac gtagtgaagc tgaacgcgaa
     6181 gcgattgttg atgagatgtt tgccaccgcg ccgcaagcga tggcaatgat ggctgagttg
     6241 gcaatacgcg gaccggagaa aattcagccg cgcgttgact ggcaagggct ggagatcatc
     6301 gaagagatgc ggcgtaataa cgagaaagtt atctttctgg tgccgcacgg ttgggccgtc
     6361 gatattcctg ccatgctgat ggcctcgcaa gggcagaaaa tggcagcgat gttccataat
     6421 cagggcaacc cggtttttga ttatgtctgg aacacggtgc gtcgtcgctt tggcggtcgt
     6481 ctgcatgcga gaaatgacgg tattaaacca ttcatccagt cggtacgtca ggggtactgg
     6541 ggatattatt tacccgatca ggatcatggc ccagagcaca gcgaatttgt ggatttcttt
     6601 gccacctata aagcgacgtt gcccgcgatt ggtcgtttga tgaaagtgtg ccgtgcgcgc
     6661 gttgtaccgc tgtttccgat ttatgatggc aagacgcatc gtctgacgat tcaggtgcgc
     6721 ccaccgatgg atgatctgtt agaggcggat gatcatacga ttgcgcggcg gatgaatgaa
     6781 gaagtcgaga tttttgttgg tccgcgacca gaacaataca cctggatact aaaattgctg
     6841 aaaactcgca aaccgggcga aatccagccg tataagcgca aagatcttta tcccatcaaa
     6901 taaaaaacag cctctcaaat tattgagagg ctgtacttga taaaaaatct caatgagtgt
     6961 tctcaccatt ttgagttttt agattagtac gctattgagt taatcgtagc gtgataattt
     7021 caaaaggttt gaattcaaga ttgcaatatt gattgcaata aaaactgtca tctaatttat
     7081 tttcttggag atcgacatat tgaacgtagt gataattaca tacccacgaa aacatggctg
     7141 aaatttctaa tccataactc tcatataatc ttacgattat accgtcgccg tcttcagcta
     7201 attttattgt ttcgatgata acgctgttag tatcgatagc aaaaaatgat agtgaagggg
     7261 gtagttcacc cgggtgtggt ttgacaggga cgatctgtaa gggatgattc attatatagg
     7321 cctggttaac gacaccaccc acacggtaat ctccatgatg aggaaataag gagaatgtga
     7381 atttttgttt accaatatct gcaaaattat taatcaggaa attatcataa ttatctgtct
     7441 taacaacttc cccaggtttg tgctgcgaac gcaacaaagt taactctagc gtctggtctt
     7501 tgacacgata gccatatttg ttttttgcta ttaaagcaat gccagtttct tgatcagaaa
     7561 ggtcgaccca gtgatgtgct ggtatttcat ctttggcaaa agcgaacgaa tcatcgctat
     7621 gcgttggtct gtcaattaca ccgaactgaa tttgacattg tgcaagagat gtttgtactg
     7681 ataccgggaa ttttactttg agagaaacat taggttcgtg ccaatcaata aaagtttgga
     7741 catcaacgcg tggactgtcc tgtgtcaggg taaatgcgaa gcgaagataa gaggaattgt
     7801 agtgataaat cagcgtcatc gtagttttag gcccgctgat cacagttgtc attttttcag
     7861 cgagtaaaac atatgattcc ccatcacgat aatttgaagc aaaatcccag gccgcgaaaa
     7921 aacctgcatc ttcgtggtat gcacggatct cac
//
LOCUS       DAZGJW010000191         7942 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZGJW010000191 DAZGJW010000000
VERSION     DAZGJW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.191"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            106..648
                     /gene="ecpR"
                     /locus_tag="ACHKIK_004492"
                     /gene_synonym="matA"
     CDS             106..648
                     /gene="ecpR"
                     /locus_tag="ACHKIK_004492"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO9085528.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            722..1309
                     /gene="ecpA"
                     /locus_tag="ACHKIK_004493"
                     /gene_synonym="matB"
     CDS             722..1309
                     /gene="ecpA"
                     /locus_tag="ACHKIK_004493"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO9085529.1"
                     /translation="MKKKVLAIALVTVFTGIGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1367..2035
                     /gene="ecpB"
                     /locus_tag="ACHKIK_004494"
                     /gene_synonym="yagY"
     CDS             1367..2035
                     /gene="ecpB"
                     /locus_tag="ACHKIK_004494"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO9085530.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2061..4586
                     /gene="ecpC"
                     /locus_tag="ACHKIK_004495"
                     /gene_synonym="yagX"
     CDS             2061..4586
                     /gene="ecpC"
                     /locus_tag="ACHKIK_004495"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO9085531.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAEVTQTGEENES"
     gene            4576..6219
                     /gene="ecpD"
                     /locus_tag="ACHKIK_004496"
     CDS             4576..6219
                     /gene="ecpD"
                     /locus_tag="ACHKIK_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO9085532.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6188..6898
                     /gene="ecpE"
                     /locus_tag="ACHKIK_004497"
     CDS             6188..6898
                     /gene="ecpE"
                     /locus_tag="ACHKIK_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO9085533.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7211..7540
                     /gene="ykgJ"
                     /locus_tag="ACHKIK_004498"
     CDS             7211..7540
                     /gene="ykgJ"
                     /locus_tag="ACHKIK_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO9085534.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7788..>7942)
                     /locus_tag="ACHKIK_004499"
     CDS             complement(7788..>7942)
                     /locus_tag="ACHKIK_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HFO9085535.1"
                     /translation="PPLFDLPWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEI
                     PLGSNR"
BASE COUNT         2072 a         2024 c         2044 g         1802 t
ORIGIN      
        1 cttccggttt actaacgaaa ctgaatgcac ctgtaaaaat tacaggtttg gaaagtagtg
       61 acatggcaaa gtgattacag cagggactat gaggttaaaa accatatgga atgtcaaaac
      121 cgttctgata aatacatctg gtctccccat gacgcctact tctataaagg actatctgaa
      181 ctgattgtgg atatcgacag attaatttat ctatcgttgg agaaaattag aaaagatttc
      241 gtgtttatca atctcaatac ggattcttta tctgaattta taaaccgtga taatgaatgg
      301 ttatccgcgg taaaggggaa acaggtcgta ttgattgcgg ccagaaagtc agaagcctta
      361 gcaaattatt ggtattacaa cagcaatatt aggggcgtgg tatacgcagg actgagtcgt
      421 gatattagaa aagaactggc ctatgtgatt aatggcaggt tcctgagaaa agatattaag
      481 aaagataaaa tcacggaccg ggaaatggaa attatccgca tgacggccca gggaatgcaa
      541 cctaaatcga ttgccagaat tgaaaattgt agtgtgaaga cagtgtatac ccatcggcgt
      601 aatgctgagg ccaagctgta ctcaaaaata tataagttgg ttcagtaaac tccaggctag
      661 ttagttttaa aaatgactca ctgggacatc acgtcctcaa ttcaactcgg gaagaaatgc
      721 aatgaaaaaa aaggttctgg caatagctct ggtaacggtg tttaccggta taggtgtggc
      781 gcaggctgct gacgtaacag ctcaggctgt agcgacctgg tcagcaacag ccaaaaaaga
      841 caccaccagt aagctggttg tgacgccact cggtagccta gcgttccagt atgccgaagg
      901 cattaaaggt tttaactcac agaaaggtct atttgacgtg gctatcgagg gtgactcaac
      961 ggctaccgcc tttaaactga cctcacgtct tatcaccaac acattaaccc agttggatac
     1021 ctcaggttcc acactgaatg tgggcgtgga ttataacggc gcggcagtcg aaaaaactgg
     1081 cgataccgtg atgatcgata ccgccaacgg cgtactgggc ggcaacctta gcccgctggc
     1141 aaacggttac aatgccagca atcgtaccac cgcacaggat ggtttcactt tctccatcat
     1201 cagcggcacc accaatggta ccaccgcagt aaccgattac agcactctac cggaaggcat
     1261 ctggagcggc gacgttagcg tacagttcga cgcgacctgg accagttaat ctctctgatg
     1321 taccagcagg ggtagccccc ctgctttatt cctggacgga attgttatga aaaagcacct
     1381 tctgcctctc gctctgctgt tttccggaat atctccggcc caggcgctgg atgtcggcga
     1441 tatatcatcg tttatgaaca gtgacagcag cacgctgagc aaaacgatca aaaacagtac
     1501 cgacagtggt cgccttatca atatccgtct cgaacggctc tcttcaccgc ttgacgacgg
     1561 gcaggttatc tcaatggaca agccggatga gttgctactc actcccgcca gcttgctgct
     1621 acccgcccaa gccagcgaag tgatccgctt cttctataag ggacccgcag atgaaaaaga
     1681 gcgctactac cgcattgtct ggtttgatca ggccctcagt gatgcgcagc gcgataatgc
     1741 caaccgcagc gctgtggcca ctgcttccgc ccgcatcggc accattctgg tcgtcgcccc
     1801 tcgtcaggcg aactaccact ttcagtacgc caacggctcc ctgacaaata caggaaatgc
     1861 gacgctgcgg atcctcgctt acggcccctg cctgaaagcc gccaatggta aggagtgtaa
     1921 agagaattac tacctgatgc caggcaagtc gcgtcgtttt acccgcgtgg acacggcgga
     1981 taacaaagga cgggttgcac tttggcaggg tgataagttc attcccgtga aatagatagc
     2041 tgtgcagatg gataacgaca atgcctttac gacggttctc cccaggactg aaagcccagt
     2101 ttgccttcgg catggtcttt ttgttcgttc aacccgatgc cagcgctgct gacataagtg
     2161 cgcagcaaat aggtggggtg attattccgc aggccttcag tcaggcgctt caggacggca
     2221 tgagcgtccc gctctatatt catctcgccg gtagccaggg tcgccaggac gatcagcgaa
     2281 tcggcagcgc ttttatctgg ctggatgatg gacagctacg catccggaaa atacagctgg
     2341 aagagagtga agataacgcc agtgtcagcg aacaaactcg acagcagctg atggctctgg
     2401 cgaacgcccc gttcaatgag gcccttacca tccccctgac tgacaacgcg cagctggatc
     2461 tcagcttgcg ccaactgctg ctgcagctgg tggtcaagcg cgaagcgctg ggcaccgtac
     2521 tacgctcacg tagcgaagac atcgggcagt ccagtgttaa caccctcagc agtaatctga
     2581 gctataactt gggcgtctat aacaaccagt tgcgtaacgg cgggagcaac acatccagct
     2641 atctgtcgct gaataacgtt actgcgctgc gcgaacatca tgtggtgctc gacggctcgc
     2701 tgtacgggat cggtagcggt caacaggaca gtgaattata taaagcgatg tatgaacgcg
     2761 attttgccgg tcaccgattt gccggtggaa tgctcgacac ctggaacttg cagtccttag
     2821 ggccgatgac cgccatttca gcagggaaga tttacggcct ttcctgggga aaccaggcca
     2881 gctccaccat cttcgacagc agccagtcag ccacgccagt gatcgccttt ttaccggcgg
     2941 cgggcgaagt acatctcacc cgtgatgggc ggctactaag cgttcagaac ttcactatgg
     3001 gcaatcatga agtggatacc cggggtctac cgtacgggat ttacgatgtg gaagttgagg
     3061 tgatcgttaa cggtcgcgtg atcagcaaac gcccccagcg ggtcaataag ctgtttagcc
     3121 gggggcgcgg cgtcggtgca ccactggcgt ggcaggtatg gggcggtagc tttcatatgg
     3181 atcgctggtc ggaaaacggg aaaaagacgc gaccagctaa agagagttgg ctagcaggtg
     3241 cctcgacctc cggctcattg agtacgctta gctgggcggc aacgggatat ggatacgata
     3301 atcaggcggt gggtgaaacc cgtctgacgc tgccgcttgg gggggcgatc aacgttaacc
     3361 tgcaaaatat gctggccagt gacagctcat ggagcagcat cggcagcatc agcgccactc
     3421 taccgggagg ctttagttcg ctgtgggtta accaggaaaa aacccgcatt ggcaatcaat
     3481 tgcgacgtag cgatgccgac aaccgtgcaa tcggcggcac actcaacctg aactcactgt
     3541 ggtcgaagct gggcacattc agcatcagct acaatgatga ccgccgttac aacagccatt
     3601 attacacggc agattactat caaaatgtct acagcggtac ctttggttcg cttggcctgc
     3661 gggccggtat tcagcgctat aacaacggcg acagcaacgc caatacaggg aaatatatcg
     3721 ctctcgatct ctcgctacca ctgggcaact ggtttagcgc agggatgact catcaaaacg
     3781 gctacaccat ggcaaacctg tcagcacgca aacagtttga tgaaggaacc attcgcactg
     3841 ttggtgccaa tctgtcacgt gccatctccg gcgataccgg tgatgacaaa accctcagcg
     3901 gtggggcgta tgcacagttc gacgctcgtt acgccagcgg aacgctgaac gtcaatagcg
     3961 cggcggacgg ctacatcaat accaacttga ccgccaatgg cagcgtcggc tggcagggta
     4021 aaaacatcgc tgccagcggg cggactgatg gcaacgctgg ggtgatattc aacaccgggc
     4081 tggaggacga cggtcagatc agcgccaaaa tcaacgggcg gattttcccg cttaacggca
     4141 agcgtaacta tctcccgctc tctccctatg gaagatatga ggtggagtta cagaacagca
     4201 aaaactcact cgacagctac gatatcgtca gcggccgcaa aagccatctg actctctatc
     4261 caggcaatgt cgctgtcatt gagccagagg tgaagcagat ggttaccgtc tccggtcgta
     4321 tccgtgcgga agacggcaca ctgctggcta acgcacggat taacaaccat atcggccgaa
     4381 cccgaaccga tgaaaacggc gagtttgtca tggacgtgga taagaaatat cccactatcg
     4441 attttcgcta cagtggcaat aaaacctgcg aagtggcact ggaactcaac caggcgcgcg
     4501 gtgccgtctg ggtcggtgat gtggtctgca gcggcctctc atcgtgggcg gaggtgacgc
     4561 agacaggaga agagaatgag agttaaccta ctaataacga tgataatttt cgcgctaatc
     4621 tggccagtaa ctgagctcag agcggcagtg agcaaaacaa cctgggcgga tgcaccggca
     4681 cgcgagtttg tgtttgtcga aaacaactca gacgacaact ttttcgtcac tcctggcggg
     4741 gcgctggatc cgcgcctgac cggtgccaac cgctggaccg gtttaaaata cactggttca
     4801 ggaaccatct atcagcaaag cctcggctac attgataacg gttacaacac cggcctttat
     4861 accaactgga agtttgatat gtggctggaa aattcaccag tttcatctcc tttaactggc
     4921 ttgcgctgca tcaactggta cgctgggtgt aatatgacca ccagtcttat cctgccgcaa
     4981 accaccgacg ccagtggatt ttatggcgcg acggtgacca gcggcggcgc gaagtggatg
     5041 cacggcatgt tgtcagacgc gttttaccag tatctgcaac aaatgcccgt cggcagcagc
     5101 tttacaatga ccatcaatgc ctgccagacc tctgtgaact atgacgccag cagcggcgca
     5161 cgctgtaagg atcaggcctc cggcaactgg tatgttcgca acgtcaccca tacgaaagca
     5221 gcaaatctgc ggttaataaa tacccactcg ctggcggagg tatttatcaa cagcgacgga
     5281 gtaccgactc tgggcgaagg gaacgccgac tgccggacac agaccatcgg cagcctttca
     5341 ggattaagtt gtaagatggt caactatacc ctgcaaacaa acggactcag caacacctca
     5401 atccatatat tcccggcgat cgccaactcg tcgttagcct cggccgtcgg ggcgtacgat
     5461 atgcagttca gtctgaatgg cagttcatgg aaaccggtga gcaataccgc ctattactac
     5521 accttcaacg agatgaagag cgcagactcg atctatgttt tcttctcgag caacttcttt
     5581 aagcagatgg tgaacctcgg gatcagcgat atcaacacca aagatctatt caactttcgc
     5641 tttcagaaca ccacatcacc ggagtctggc tggtatgaat tttctacctc caacacgctg
     5701 attatcaaac cccgtgattt cagcatcagt attatctccg atgaatatac tcagacaccg
     5761 tcgcgggagg gatatgttgg cagcggcgag tcggcactcg atttcggcta tatcgtaacc
     5821 accagcggta aaacagctgc cgacgaagtg ctgatcaagg tgaccggacc cgcgcaggtg
     5881 attggcgggc gctcctattg tgtcttcagc tccgatgacg gtaaggcgaa agtaccgttc
     5941 ccggcgacac tttcctttat tacccgcaac ggagctacaa aaacctacga tgccgggtgc
     6001 gatgatagct ggcgggatat gaccgatgcg ctgtggttga ccacaccgtg gactgatatc
     6061 tctggcgaag tggggcagat ggataagacc acagtcaaat tttcgattcc aatggataac
     6121 gccatttcgc tgcgtacggt agatgataac ggctggtttg gcgaagtcag cgcttcagga
     6181 gagattcatg ttcaggcgac gtggcgtaac attaactaag gccctgctga cagcggtctg
     6241 tatgctggcg gcacctttga cacaggcgat ttcggtcggc aatctgacat tttcgctgcc
     6301 gtccgagact gactttgtca gcaaacgtgt agtgaataac aacaaaagcg cacggatata
     6361 ccgtattgcc atcagtgcta ttgatagccc gggcagcagt gaattgcgca cccgaccggt
     6421 ggatggtgaa ctgctttttg ccccccgcca actggcgttg caggctggtg agagcgagta
     6481 ttttaaattt tactatcatg gtccacggga taaccgcgag cgctactacc gggtctcatt
     6541 tcgcgaagtc cccactcgta accagacaag acgtagccca accggcggcg aggtcagcac
     6601 ggagccggtg gtggtgatgg ataccattct ggtagtacga ccacgtcagg ttcagtttaa
     6661 atggtccttc gacaaggtga caggaacggt aagtaacact ggcaacacat ggtttaagct
     6721 actgattaag ccaggatgtg attcgaccga agaggaaggc gatgcctggt atctacgtcc
     6781 gggagacgtg gttcatcagc ctgagttacg tcagccgggg aatcattatc tggtctataa
     6841 cgacaaattc attaagatta gcgattcctg tccggctaag cccccttcgg cggactaaga
     6901 cttatccatc ggtcgaggga agaattccac ctcagagctc caaaaatcgt ttaaatgatg
     6961 tggaagcgat cgtcaatgtc acctgtaacg atgggctttc tcatatgcac accatagtgt
     7021 tcaggagaca cggaaaggta tcaggcagcc tgctaatgag tggtttaatc acgaattagt
     7081 acgtaaaatc ggtaacggct ggaaatcatt caatactcac actatcgaaa gttccccagc
     7141 caaccgcggt acgttcttac atacgatgta ccgctgttct ctttacgatt tttagctgta
     7201 ctggtgaatt atgagcaatc tgaatccatg catgacgtgt ggtgcctgtt gtgcattttt
     7261 ccgcgtctct ttttactggg ccgaagccga cgatgctggc ggtactattc ccgccaggct
     7321 cactgaacaa atatcccctt ttcaccgatg catgagcggt accaatcaga aaaacccccg
     7381 atgtattgcc cttgcaggaa ccccgggcaa aaatgcctgc tgcacgatat ataaaaatcg
     7441 atcgtccaca tgcagagaat ttgccatgtc tggtgagaac ggagtcgtca atgaggcttg
     7501 caatcgtgca agggctaaat acgggctgac accgttataa acatacaata attaattgca
     7561 ctgccccgcc agcgataata gcggggcttc gtttttcagg ggtaacaaaa cccgatactt
     7621 cttctatttg ccagcaacaa tgcctctctt ctgtagcgtt cttcggatct acatcatcct
     7681 gaagtagcgc gatatactca ctgactatct gaatcagaat ataaaaagca atgtttttaa
     7741 cctataaaaa tggcgctgta tttgcgccat ttttatcatt caatgcatta tctgtttgag
     7801 cctaaaggga tctcagggtc tggctcatga gtaattctgt ttcgtaaatc tctgcgaata
     7861 atttcaatag accagaacca gactaaatgt ccaaaaattt cagaaacatt ctcataccac
     7921 gggagatcaa acagaggtgg cg
//
LOCUS       DAZGJW010000192         7883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZGJW010000192 DAZGJW010000000
VERSION     DAZGJW010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.192"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..5179
                     /locus_tag="ACHKIK_004500"
                     /pseudo
     CDS             2..5179
                     /locus_tag="ACHKIK_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            5402..5668
                     /gene="hipB"
                     /locus_tag="ACHKIK_004501"
     CDS             5402..5668
                     /gene="hipB"
                     /locus_tag="ACHKIK_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO9085536.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            5668..6990
                     /gene="hipA"
                     /locus_tag="ACHKIK_004502"
     CDS             5668..6990
                     /gene="hipA"
                     /locus_tag="ACHKIK_004502"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO9085537.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            7302..7510
                     /locus_tag="ACHKIK_004503"
                     /pseudo
     CDS             7302..7510
                     /locus_tag="ACHKIK_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2174 a         1673 c         2143 g         1893 t
ORIGIN      
        1 tatgaataga atctatcgcg tgatatggaa ttgcactcta caggtatttc aggcctgctc
       61 ggaattaact cgcagggcag gtaaaacatc gacggttaat ttgcgtaaat cctctggact
      121 gacaacgaaa ttcagtagat tgacgctggg tgttttgctg gcactaagcg gttcagcgtc
      181 tggtgcaagt ctggaagttg ataatgatca gattaccaat attgatactg atgttgctta
      241 tgatgcctac ctggttggct ggtatggcac tggagtgctt aatattttgg ctggcggtaa
      301 tgcctcctta accactatta ctaccagcgt cattggcgct aatgaggact cagagggcac
      361 cgttaatgtt ttgggtggca cctggcgatt gtatgatagc ggaaataatg caaggccttt
      421 aaatgtgggt caatccggaa cggggacgct gaatattaaa cagaagggtc acgtcgatgg
      481 aggctattta agattaggtt cttcgacagg aggcgtcggg acggtcaatg ttgagggaga
      541 ggactctgtt ctgacgaccg aattattcga aatagggagt tacggtacgg gctcattaaa
      601 tattacggat aagggttacg tcacgagttc aatcgtcgcc attttaggct atcaggcggg
      661 cagtaatggc caggttgtcg ttgaaaaggg tggcgagtgg ctaataaaaa ataacgattc
      721 ctcaattgaa tttcaaattg gtaatcaagg aactggggag gcgactattc gcgagggtgg
      781 actgattacg gctgaaaata cgattatcgg tggcaatgcc accggtgtcg gaaccctgaa
      841 tgtgcaggat caagactctg tcatcacggt acgcagactc tataatggat atttcggtaa
      901 tggcacactc aatatttcca ataatggact aattaataac aaagaatatt cattagtggg
      961 cgttcaggac ggttcccacg gtgtcgtcaa cgtgaccgat aaagggcatt ggaatttcct
     1021 cggaacgggc gaagctttcc gctatatcta tatcggtgat gctggcgacg gtgaacttaa
     1081 tgtctcgcgt gaaggtaaag tagattcggg aattatcact gcggggatga aagaaacagg
     1141 cacaggcaac attactgtta aggataagaa ctccgttatc actaatctcg gaactaatct
     1201 tggttatgac ggccacggcg aaatgaatat cagtaatgaa gggcttgttg tcagcaacgg
     1261 aggaagctca ctcggttatg gagaaaccgg cgtcgggaat gtcagcatca ccacgggggg
     1321 gatgtgggag gtcaataaga atgtctatac caccattggt gttgcgggcg tcggaaacct
     1381 caatattagc gatggcggta agttcgtatc gcaaaatatt acttttttgg gcgataaagc
     1441 aagcggtatc ggcacactga acctgatgga tgcgacatca tcgttcgata ctgtgggtat
     1501 caatgtcggt aattttggta gcggtatcgt aaatgtcagt aatggtgcca cccttaattc
     1561 aacgggctat ggatttatcg gaggaaatgc ctccggtaag gggatagtta atatttcaac
     1621 ggacagtctc tggaatttaa agacatcatc tactaacgca caattgctac aggtcggtgt
     1681 attaggcaag ggtgaactga atattaccac cggaggtata gttaaagcgc gtgatacaca
     1741 gatagctctc aatgacaaaa gtaagggcga cgtgagggtg gatgggcaga actctcttct
     1801 tgaaacattc aatatgtacg tagggacatc tggtacgggt acgttaaccc tgacgaatag
     1861 cggcacgctg aatgtcgaag gtggagaagt ttacttaggt gtttttgaac ctgctgtagg
     1921 aacgctaaac attggtgcag ctcacggtga ggcggcggca gatgccgggt atataaccaa
     1981 cgcgacgaaa gtagagtttg gttctggcga aggtgttttt gtctttaatc atactaataa
     2041 cagtgatgcc ggctaccagg tcgatatgct gattacaggt gacgataaag acggaaaagt
     2101 gatccatgat gcaggccata cggtgttcaa tgcagggaat acttatagcg gtaaaacgct
     2161 ggtcaatgac ggcctcctga ccattgcgtc tcatacggca gatggggtaa cgggcatggg
     2221 ttcgagtgaa gtcaccattg caagccccgg tacgctcgac attctcgcat caacgaacag
     2281 tgcaggagat tacacgctga ccaatgcgct caaaggcgat ggcttgatgc gagtgcagct
     2341 gtcatcctac gacaagatgt ttggctttac ccatgcaaca gggactgaat tcgccggtgt
     2401 tgcccaactg aaagacagta ccttcactct ggaacgcgac aacaccgctg cgcttactca
     2461 cgcgatgttg cagtctgaca gtgaaaatac cacatcggta aaagtgggag agcaatccat
     2521 tggtggactg gccatgaatg gcggtacgct cattttcgat acggatattc ctgctgcgac
     2581 gcttgcagag ggatatatca gcgtcgatac gctggttgtc ggcgcgggtg actacacctg
     2641 gaaaggccgt aactatcagg taaacgggac gggcgacgtg cctaaaccgt ggaatgatcc
     2701 catggcgaat aaccctctga cgacgctcaa tttgctggaa cacgatgata gccatgtcgg
     2761 cgttcaactg gtgaaggcgc aaacggttat tgggtcgggt ggctcattaa cgttacgtga
     2821 tttacagggc gacgaggtgg aagcggacaa aacgttacac attgcgcaaa acggaacggt
     2881 ggtcgccgag ggtgattatg gattccgcct cacgaccgca ccaggtgatg gtttgtacgt
     2941 taactatggg ctgaaagcgc tgaacatcca tggtgggcaa aagctgacgt tagccgaaca
     3001 tggcggagcc tatggcgcaa cggccgatat gtcggcaaaa atcggtggtg aaggggatct
     3061 ggcaatcaat acggtgcgac aggtttcgct ttccaacggt cagaacgact atcagggggc
     3121 aacctacgtt cagatgggga cattacgtac cgatgcggat ggtgcacttg gcaacacccg
     3181 ggaactgaac atcagcaacg cggccatcgt cgatcttaat ggatcgacgc agacggtaga
     3241 gacattcacc gggcagatgg gttcgactgt tttgttcaaa gagggggcgc tgacggtaaa
     3301 taaaggtggg atcagtcagg gtgaactgac aggtggcgga aacctgaatg ttacaggggg
     3361 aacgctggct atcgaggggc ttaatgcacg ctacaatgcg ttaaccagca ttagcccaaa
     3421 tgcggaagtc agcctcgata atacgcaggg gttaggcaga ggaaatattg ccaataacgg
     3481 tctgttaacg ctaaaaaacg tgactggcga actgcgcaat agcataagcg ggaagggtat
     3541 cgtgagcgca accgccagga cagatgtaga gctggatggc gataatagcc gctttgtggg
     3601 gcaattcaac attgatacag gcagcgcgct cagcgtcaac gagcagaaaa acctgggtga
     3661 tgcttccgtt atcaataatg gcctgctcac catctccact gagcgtagct gggcgatgac
     3721 gcacagtatc agcggtagcg gtgatgtgac aaaactgggt accgggatcc tgactcttaa
     3781 caacgattcc gcggcgtatc agggtacgac ggatatcgtg ggggggggga aattgctttc
     3841 ggttccgact ctgccattaa tatggcaagt caacacatta atatccataa cagcggtgtg
     3901 atgtcgggaa atgtcaccac tgcaggtgat gtgaacgtta tgcctggggg gacactgcgt
     3961 gtcgctaaaa ccacaatcgg cggcaacctg gagaatggcg gcacggttca aatgaatagc
     4021 gaagggggga aaccggggaa tgtactgacc gttaacggca actataccgg aaacaatggc
     4081 ctgatgacgt tcaacgcgac gctgggcggc gataattcgc ccaccgataa aatgaacgtg
     4141 aaaggcgata cccaagggaa cactcgcgtt cgggttgata acattggcgg cgtcggtgca
     4201 caaacggtca acggtattga actcattgag gttggcggta attctgcagg taacttcgcg
     4261 ctgaccaccg gaactgtcga agctggggct tacgtctaca cgctggctaa agggaagggg
     4321 aatgacgaga aaaactggta tctgaccagt aaatgggacg gcgtaacgcc agcggataca
     4381 cccgatccca tcaataatcc ccctgttgtg gatccggaag gcccatcagt ttatcgcccg
     4441 gaggccggaa gctatatcag caacattgcc gcagccaact cgctgtttag ccatcgttta
     4501 cacgaccgtc tgggtgagcc gcagtatata gattcactgc attctcaggg gtcggcaagc
     4561 agtatgtgga tgcgtcatgt cggagggcac gaacgttcaa gggccggtga cggtcagcta
     4621 attactcagg ctaaccgcta tgtattgcag ctaggcggcg atttggcgca gtggagtagc
     4681 aacgcgcagg atcgctggca tgacggcctc tgttgagggt gggtatacct ttgaagcggg
     4741 aacatttaga ggcagcgaag ggacgctgaa tacctggtac gtccagccac aggcgcaaat
     4801 cacctggatg ggtgtgaaag attctgacca taatcggaaa gacggaacac gcattgaaac
     4861 ggaaggcgac ggaaacgtgc aaacgcgact tggggtgaaa acctacctga atagccatca
     4921 caagcgtgac gatggtaaac agcgtgagtt ccagccttac attgaagcga actggatcaa
     4981 caatagcaaa gtctacgccg tgaagatgaa tggtcaaacc gtaagccgtg atggtgcgcg
     5041 taatctcggt gaagtacgta ccggggttga ggcgaaagta aataacaacc ttagcctgtg
     5101 ggggaatgtc ggtgtgcaac taggtgataa aggctatagc gatactcagg gcatgctggg
     5161 agtgaaatat agctggtaaa ccgtataagc cgcatgtcga gatggcatgc ggcttaatat
     5221 tgccgacttc aaacggcgca tcagcgcctt ttttaaatcc tcctttttat ccgcgatcgc
     5281 ggatatcgca gcgtttatcc cgtagagcgg ataagacgtg tttccaaatt gacttatcct
     5341 cactaaagga taaaactgat aatatcctct tatgcggata aatttgctgt ggacgtatga
     5401 catgatgagc tttcagaaga tctatagccc aacgcaattg gcgaatgcaa tgaaactggt
     5461 tcgccagcaa aatggctgga cgcagagcga gctggcgaaa aaaattggca ttaagcaggc
     5521 gacaatttcc aatttcgaaa acaaccctga caataccacg ctcacgacat tttttaagat
     5581 tttacagtcg cttgaactct caatgacact atgcgacacg aaaaatgcct cgccagaatc
     5641 aacagaacag caagatctgg agtggtaatg cctaaacttg tcacttggat gaacaaccag
     5701 cgggtaggcg agttaacgaa gttagccaac ggcgcgcaca cctttaagta tgcaccggag
     5761 tggttagcca gccgttatgc cagaccgttg tcactttcgc tgccattgca gagggggaat
     5821 atcacctcag atgccgtatt taacttcttc gataacctgt tacccgatag cccgattgta
     5881 cgtgaccgga tcgttaaacg ttatcatgct aaatccagac aaccgtttga tttattgtca
     5941 gaaatagggc gagacagcgt tggtgccgta acgttattac ccgaaaatga aaccataacg
     6001 cgcccgataa tggcatggga aaagcttact gaagccaggc ttgaagaagt attaacggct
     6061 tataaagcag atatccctct gggcatgatt agagaagaaa atgactttcg catctcggtt
     6121 gctggcgcgc aggagaagac agcgctgctt agaataggca atgactggtg cattccgaaa
     6181 ggaataacgc cgacgacgca catcattaaa ttaccgattg gcgaaatcag acagcccaat
     6241 gcgacgctcg atctcagcca aagcgttgat aatgagtatt actgtctgct gctggcgaaa
     6301 gaacttgggt tgaatgttcc ggacgcagaa atcattaaag cgggaaatgt gcgcgcgtta
     6361 gcggtcgaac gttttgacag gcgttggaat actgagcgaa cggttttact tcgcttgcca
     6421 caggaggata tgtgtcagac attcggttta ccttcatctg tgaaatatga atcagatgga
     6481 ggcccaggca tcgcgcggat catggcgttt ttgatggggt ccagcgaggc gcttcgcgat
     6541 cgttatgatt ttatgaaatt ccaggtcttc cagtggttga ttggcgcaac ggatggccat
     6601 gcaaaaaact tctccgtatt tattcaggct ggcggcagtt atcgactcac gccattttac
     6661 gacatcattt cagcatttcc ggtccttggc ggtacgggaa tacacatcag cgatctcaaa
     6721 ctggcaatgg ggcttaacgc atccaaaggc aaaaaaacgg caatcgataa aatttatccg
     6781 cgacattttt tggcgacagc aaaggtgctg agattcccgg aagtgcagat gcatgaaatc
     6841 ctaagtgact ttgccagaat gattccggca gcactggata acgtgaagac ttcattaccg
     6901 acagattttc cggagaacgt ggtgacggca gttgaaacca atgtgttgag gttgcatgga
     6961 cggttaagcc gagaatacgg tagtaagtga gatatggggt ttggtcgttg ttaatgagca
     7021 cgacaatcat gaccgccaac gactggattc ttccttggtt tatactggtg cgcataatgt
     7081 atattatgtt aaattatgtc tgagtgatgc gaagtgcttt tctggtagtc gttattcgtt
     7141 cagggttcaa gaacttaagc ctctcctctt tcataattgg cctgtgcatg gagaaatagc
     7201 ctggttattc cctgccccat gctaagagcg cagtgtctgt cgcatagaca ctgcatacga
     7261 aaacaccaga gggtgctgtt acttatttaa ggacggatca agcgggaatg agtccactcc
     7321 ggtatcagaa atggctacgt ctcaatgaag tcaggcgacc gatgctgaat gaacattacg
     7381 atgtcaccac agcagcctat gctgtcggtt acgaaagcta tcccatttca gtcgggaata
     7441 ttcgcggatg tttggagagt cacccaagag agatattacc aggttaagaa agtctgtcgg
     7501 tcagctttaa taaaccctgc acttatctgt ttagagcaat tcggtgttag tttcagcaag
     7561 caaacattaa ccatagctaa tgatttatag ccatattaac cattggtagc ccttgacctt
     7621 tcctgacact tcccttgttc gcaataatat tctgtttagt cacggtttat aaataacatg
     7681 cataatgaaa tcgtcatgtt atttagcgac ataaaatgtt tttgcaaata ctctcttcaa
     7741 caagtgcaca ataaacttgt tgtgattagg tgtccttgtg atttaaagat attatcgttc
     7801 ttcgtgaaat gtaataaccc tggaaactat attctcaggg caacaaccaa tcaagaatga
     7861 ttcttgtttt taagcaataa cag
//
LOCUS       DAZGJW010000193         7850 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZGJW010000193 DAZGJW010000000
VERSION     DAZGJW010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.193"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(6..140)
                     /locus_tag="ACHKIK_004504"
     CDS             complement(6..140)
                     /locus_tag="ACHKIK_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085538.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLIRAAKKQLEALCAMK
                     "
     gene            complement(216..527)
                     /locus_tag="ACHKIK_004505"
     CDS             complement(216..527)
                     /locus_tag="ACHKIK_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion protein"
                     /protein_id="HFO9085539.1"
                     /translation="MKLRVWHIPQVPMKPFIAEVASVEEGVRLMGALADYDAFQYDNN
                     IKPDYCNANGLEMWDESLTDEDLSEVGLTDRWVDWYSECQCYDDPRKYLESLKEETSA
                     A"
     gene            complement(671..1141)
                     /locus_tag="ACHKIK_004506"
     CDS             complement(671..1141)
                     /locus_tag="ACHKIK_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085540.1"
                     /translation="MTKSWSVPFPESETKHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQKRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
     gene            1342..1836
                     /locus_tag="ACHKIK_004507"
     CDS             1342..1836
                     /locus_tag="ACHKIK_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085541.1"
                     /translation="MDVLSGITAAKQAYDLLKTIKETRDDAVIAKAIGDLHQRITDLQ
                     MLNAELSGLYQAEKEIAMKLRDENRKIKMFVVQAENYELHTTEGGSVVYRPKSHSDPS
                     IQQHNLCAHCFGEHKISILQPSTVTIKSNGFFVHSCPRCKNEYRMYRAPDPKPVYVPP
                     LTNY"
     gene            complement(1867..2229)
                     /locus_tag="ACHKIK_004508"
     CDS             complement(1867..2229)
                     /locus_tag="ACHKIK_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination N domain protein"
                     /protein_id="HFO9085542.1"
                     /translation="MGQEEKYELKKLIGEDAIEEIAALTTAIKNIRYALNTLISSCDK
                     NSREFLILGAALGIVDAATLHLITHDDILIEPYETLLLVRQKMADAAANGDLQLYIDL
                     RKVLRRMVKTEGDIPLTK"
     gene            complement(2232..2504)
                     /locus_tag="ACHKIK_004509"
     CDS             complement(2232..2504)
                     /locus_tag="ACHKIK_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085543.1"
                     /translation="MTVVITYLADDNARNRRRARRQAQREQAMQEQRLARKIALKLSG
                     CVRADKAASLGSLRCKKADECSGSICLPNVAIYAAGYRKSKQLTAR"
     gene            complement(2938..3234)
                     /locus_tag="ACHKIK_004510"
     CDS             complement(2938..3234)
                     /locus_tag="ACHKIK_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085544.1"
                     /translation="MEFLIVFVVVLVIILFVLLSISKKLSQMIEHSSNRAKEEEHIID
                     IKEILSDIKITLDEIKYTTDLIEQYKIPTPNERKAIDQYRIDLEIDEMLSKRKD"
     gene            complement(3275..3982)
                     /locus_tag="ACHKIK_004511"
     CDS             complement(3275..3982)
                     /locus_tag="ACHKIK_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO9085545.1"
                     /translation="MKTFAERLNAAMSSAGVSQSQLADMVGISQPAIQKMSSGKTNGS
                     RKMVELANALKVRPEWLSSGIGEMRDGAHEEPSNVRDSSLKAVVWEDIKRNDDEFVAL
                     PLLNVSLSAGSGSCELEESSEFSLVFRKHYLKKMGVSERSAKLVRVVGQSMEPTLHDG
                     DVVGVNTQDTTIRDGKTYAICQSDLLRVKTLIATPTSVIIRSINREEYPDEVMDRDEF
                     HETVRIIGRVFWSSHSW"
     gene            4094..4318
                     /locus_tag="ACHKIK_004512"
     CDS             4094..4318
                     /locus_tag="ACHKIK_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9085546.1"
                     /translation="MNLVIQRALKIVGSQKRLADKCGVTQPAVHKWLKGGLVSPEKVT
                     AIVNATGGQIKAYEIRPDLPHLFPKPNQAA"
     gene            4428..4706
                     /locus_tag="ACHKIK_004513"
     CDS             4428..4706
                     /locus_tag="ACHKIK_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFO9085547.1"
                     /translation="MELTSTRKKANAITSNILNRIAVRGQRKVADALGINESQISRWK
                     DSFIPKMAMLLAVLEWGVEDEELAELAKKVAMVLTKEKPQDCGNSFEA"
     gene            4742..4903
                     /locus_tag="ACHKIK_004514"
     CDS             4742..4903
                     /locus_tag="ACHKIK_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085548.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            4890..5711
                     /locus_tag="ACHKIK_004515"
     CDS             4890..5711
                     /locus_tag="ACHKIK_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO9085549.1"
                     /translation="MGVVKLADYRPQLEVVEHRVADTEDGFMRVANEIIDSLLMADLT
                     VRQMKVMLAIMRKTYGFNKPMDRLTNTQIAAMTGIHHTHVCAAKRQLIERKFLIADGV
                     KIGVNKVVSQWISQDSLTLAKTANKTLAESANGYKPSQLNTKDNIQKTINTNTPLPPN
                     GGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQLKTP
                     NVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA"
     gene            5708..7084
                     /locus_tag="ACHKIK_004516"
     CDS             5708..7084
                     /locus_tag="ACHKIK_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO9085550.1"
                     /translation="MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDTSSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERNKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGPLGTVYQRFCNGHFVACDQDEARQICTASNAPASRGR
                     RYAQGADV"
     gene            7171..7386
                     /locus_tag="ACHKIK_004517"
     CDS             7171..7386
                     /locus_tag="ACHKIK_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085551.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPIDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDLK"
BASE COUNT         2107 a         1715 c         1826 g         2202 t
ORIGIN      
        1 aatggctatt tcattgcgca taacgcctcc agttgtttct ttgctgctct aattaattgt
       61 ttaactcggc gtgataattc agattcgtgc gggtagaaag cggacatgac gccgctaccc
      121 gcgagctgaa agtgcatcat gggtaactcc ttatatttga ttgcataacg aaaacgcctc
      181 gagtgaagcg ttattggtat gcggtaaagc cgcgcttagg cggctgatgt ttcttctttc
      241 aggctttcga gatatttacg tgggtcgtcg taacattggc attcgctgta ccaatccacc
      301 cagcgatcag taagccccac ctctgataaa tcttcatcgg taaggctctc atcccacatc
      361 tcaaggccgt tagcattgca gtaatcaggc ttgatgttgt tgtcatactg aaaggcgtca
      421 taatcagcca gtgcgcccat caggcgaaca ccctcttcaa cacttgccac ttctgcaatg
      481 aacggtttca taggtacttg cgggatatgc cagacacgta atttcatatt tcctccgtca
      541 aaaaaatcgc cctcacattg gagggcaaag aagatttcca ataatcagaa caagtcggct
      601 cctgtttagt tacgagcgac attgctccgt gtattcactc gttggaatga atacacagtg
      661 cttattcgta ctaataaaat acccaatttt ctgtttcttg gttgtgtcca aagttatatt
      721 caatatctgg tgttgatgta tcaatatttt tcatcccatc aacaagagtt gatacaacag
      781 ccaaatcttg ttttattctc attaaatggt atttcttccg gcgcaataaa ctttcaatag
      841 caagtttctt cgttgggaat gcaaaagatc tttctgcatt ttttgctact ttcttaattg
      901 catatctatt tctcttttgt ttccattcct gtaaccactg atttggtgct ggtttaaaat
      961 taacaatcca atgcgcagga accaaccatg cataatgctc tgtctgatga aaagctatat
     1021 attgaagtgc gaatattttg attccatctt cttcaactgt cgcctggaat ctccagaaaa
     1081 caggcattcc atcatgttta gtttctgatt caggaaaagg tacgctccat gattttgtca
     1141 tatctcacct caaataagtg gtttgctgtc taatttcatt ttctggcgac caacacaagt
     1201 cacacccatt tcactgcgtg gcttgcggta gtaaagattg tgcctgtctt ttaaccacat
     1261 caggctcggt ggttctcgtg tacccctaca gcgagaaaaa tagtaaaatc ctcttacccc
     1321 tacagtaaga gagtaattta tatggatgtg ttatctggta taactgccgc gaaacaggct
     1381 tatgatctgc tgaagacaat aaaagaaacc agagacgatg ctgttatcgc taaagctatt
     1441 ggtgatctac accaaagaat aactgactta cagatgctta atgcggagct ctctggcctt
     1501 tatcaggccg agaaggagat cgcaatgaag cttcgagatg aaaatagaaa aatcaagatg
     1561 tttgttgtgc aagctgagaa ttatgaactt catacaactg aaggtggttc tgtcgtgtat
     1621 cgacctaaga gtcattccga tccttctatt cagcagcata acctttgtgc acattgcttc
     1681 ggcgaacata aaatatcgat acttcaacca agcacggtta ctataaaatc taatgggttc
     1741 tttgtgcatt catgtcctcg ctgtaagaat gaataccgga tgtatagagc gccagaccct
     1801 aagcctgtat atgtaccgcc gctgacaaat tattaatttt attcctgaaa tggcctcttc
     1861 acccccttac tttgtcaggg ggatatctcc ttcagttttg accattcgcc ttaatacttt
     1921 ccttaagtcg atgtaaagtt gaaggtctcc atttgctgcg gcatcagcca ttttttgcct
     1981 gacaagcagt aatgtttcat acggctcaat aagaatatcg tcatgagtaa ttaggtgaag
     2041 cgttgccgca tcaactattc ctagagctgc gccaagtatc aaaaattccc tgctattttt
     2101 gtcgcatgag gagataagcg tatttagcgc atacctaata ttctttatag ctgttgttaa
     2161 tgctgcaatt tcttctatgg cgtcttctcc aatgagcttt ttaagctcat atttttcttc
     2221 ctgacccata attacctcgc cgtcagttgt tttgatttcc ggtagcctgc cgcgtaaatg
     2281 gctacgtttg gcaggcaaat acttccactg cattcatctg ccttcttgca gcgaaggctt
     2341 ccgagtgatg ctgctttatc tgctctgacg caaccagaga gctttagcgc aatttttcgt
     2401 gccaatcgct gttcttgcat tgcctgttca cgttgagcct gtctgcgtgc tctgcggcga
     2461 tttctggcgt tatcgtcagc cagatatgta atgactactg tcatgtttac ctccgatgat
     2521 tgactttggc ggtgacgcgc cgcgtgctta tcttccggtt gccgtcttgc agctgcactt
     2581 cacgtcaccc caaagccaac tactctttgg ttcccgcatt tcggcgggac aatcccatca
     2641 atgttaaaga gcctgccaat ctgttccgtt tggcttccag cgtcctgctg atggcttaaa
     2701 gataacttag gttataggtg tggtcaataa cctaatttat attttatggt aaataggtta
     2761 taagtgatgg ataacaaagg tattttattt ttgtaaatgt tgctgattga ttggtgtttg
     2821 agggcttgcg tgcggggtga aggtgttacc tttggcttga tgcttgtcta tgatgaggat
     2881 ggttgattgg gtggtgaacg gcaggaaaag aaaacccggc gctgaggccg ggtgttttta
     2941 gtcttttctt ttgcttagca tttcgtcgat ttctaagtca atacgatatt gatctattgc
     3001 ttttctctcg tttggggttg gtattttata ctgttcaatt agatctgttg tgtattttat
     3061 ttcatctaat gtgattttta tatcagagag aatctctttt atatcaatta tatgctcttc
     3121 ttcttttgca cgattagagc tatgttcaat catttgagat agctttttac taatgcttag
     3181 caaaacaaaa agaatgatga ccaaaacaac aacaaaaact atcagaaatt ccattatccc
     3241 tccgcacttc cgtaagtctt cttctgatta tcggttacca actatgagac gaccagaata
     3301 ctctgccaat aatccttacg gtttcatgaa attcatctct atccattact tcatccgggt
     3361 actcttcgcg atttattgat ctgattatca ccgatgtagg ggtggcgatt aatgttttta
     3421 ctcgtaacaa atcagactgg caaatagcgt aggttttacc atctctgatt gtggtatctt
     3481 gcgtgttaac accaacaaca tcgccatcgt gaagcgttgg ttccatgctt tgccctacaa
     3541 ccctaactag cttggctgat ctttcagata ctcccatctt tttcagatag tgctttctga
     3601 aaaccaaaga gaactccgat gattcctcta gctcgcagct accgcttcca gctgaaagcg
     3661 aaacgttaag aagaggcaac gcaacaaact cgtcatcgtt tcttttaatg tcttcccata
     3721 ccacagcttt taaagatgaa tcacggacat tggatggttc ttcatgtgca ccatccctca
     3781 tttcaccaat accagaacta agccattcag ggcgcacttt taaagcattg gctaattcaa
     3841 ccatcttgcg agatccgttt gttttaccgg acgacatctt ctgtatggct ggctgagata
     3901 ttccaaccat gtcagcaagc tgtgattgtg atacccctgc tgagctcatg gctgcattta
     3961 gtctttctgc gaatgttttc atacccacaa atctataact acggttatcc aaagtaaaat
     4021 aacaaaggtt attgctgttt tttataactt gagttatctt tggttataag taatgaccac
     4081 aagaggtatg ctcatgaatt tagtaattca acgagccttg aaaattgtcg gtagccaaaa
     4141 gcgccttgcc gacaagtgtg gtgtaacgca gccagcggta cacaaatggc tgaaaggcgg
     4201 gttggtctct ccagagaaag ttaccgccat cgttaacgcc actggagggc agatcaaggc
     4261 ttacgaaatt cgccccgatt tgccacacct gtttccaaaa ccgaatcagg cagcataagt
     4321 aacaccgctc tttaacagtc atggtcctca ttcccgccga aatgcgggaa tacaacgcgc
     4381 ataagttgat gcgcataact tcttatttgt taaggaaata cttacatatg gaacttacaa
     4441 gtactcgcaa gaaagcgaat gcaattacaa gcaacatcct gaatcgaatt gctgtacgtg
     4501 gtcagcgaaa ggttgctgat gcattaggga tcaatgaatc gcaaatttcg cgatggaaag
     4561 acagctttat cccaaagatg gccatgcttc tggctgtgct ggagtggggt gttgaagacg
     4621 aggaattagc ggagctggca aagaaagtag ccatggtgct gacaaaagaa aagcctcaag
     4681 actgcggcaa cagttttgag gcctgattgt agaaagactg tatcaatcca caggagtaat
     4741 tatgacaaaa cgtcgtaaga aataccagga aaaagaagag attcgacacc ctgattcacc
     4801 tgagggatta gtggtagccg cagcaaataa cagggcgttc gcagagcgcc ttgttggtgt
     4861 ttacagacta gccaaagcag gagtgaaaca tgggcgtcgt taagttagct gattacaggc
     4921 ctcaactgga ggtcgtggag catcgcgtgg cagataccga agatggtttc atgcgcgttg
     4981 ctaacgagat tatcgacagt ctgctgatgg ctgatttaac cgtccggcag atgaaggtga
     5041 tgctcgctat catgcgcaag acatacggat tcaataagcc gatggatcga ctcacaaaca
     5101 cgcagatagc agccatgaca ggtattcatc acactcatgt ttgcgctgcc aagcgccagc
     5161 ttattgagcg taaattcctc attgctgatg gcgtgaaaat cggagtgaac aaggtggttt
     5221 ctcagtggat tagccaggac agcttaacat tagctaaaac agctaataaa acattagccg
     5281 agtcggctaa tgggtataag ccaagtcagc taaacacaaa agacaatata caaaagacaa
     5341 taaatacaaa taccccctta ccccctaacg ggggcggcga tgggcaggtt aaacctgaac
     5401 gtcgcaaggc agaacgaatc gactacgaat ccttcctgaa cgcctacaac accgaagtcg
     5461 gtgacagact tccacacgct gttgcggtca acgagaaacg aaaacgccgc ctgaagaaaa
     5521 tcatcccgca actgaaaacg ccaaacgtgg acggtttcag agcgtatgtc agggcgtttg
     5581 tgcatcaggc caagccgttt tacttcggag acaacgacac gggctggacg gcagattttg
     5641 attacctgct gagggaagac tcgttaacgg gagttcggga agggaagttt gcagacaggg
     5701 ggattgcatg agacaggata tcgaagcgag cgttatcggt ggcttgctga ttggtggatt
     5761 aacaccaacc gccagcgacg ttctggcaac gctggagccg gaagcgtttt caattccgct
     5821 ctaccggaaa gccttcgaag ttatccgtaa gcaggcgaga aacagaaacc taatcgacgc
     5881 gctgatggtt gccgaggcgt gcggagagga gcatttcacg tcaatcctga tgaccagcaa
     5941 aaactgcccg agtgccgcaa acctgaaggg atatgccgga atggtcgcgg ataactatca
     6001 ccgccgtctg gtgctggaaa tcatggacga aatgcgtgaa ccaattcaga gcggaaccat
     6061 cgatacatcg agtcaggcga tggacgagct tgtaaagcgt ctctcagcca tcagaaagcc
     6121 ccgtgacgag gttaaacctg tacggttagg ggaaatcatc actgactaca ctgacacgct
     6181 tgacaggcgt ctgaggaacg gagaagagtc agataccctg aagaccggaa tcgaagaact
     6241 tgacgccatc accggaggga tgaacgcgga agacctggtg attatcgccg ctcgtcctgg
     6301 tatggggaaa accgaactgg cgctgaagat tgccgaaggt gttgcaagcc gcgttattcc
     6361 tggttctgac gtccggcgcg gagtgttgat tttctcgatg gaaatgagcg cattgcagat
     6421 tgcagagcgg agcattgcca acgccgggag gatgtcggtt agcgtactgc gaaatcctgc
     6481 atcgatggat gacgaaggct gggcgcgtgt tgctaacggc atgagtcagc ttgcagattt
     6541 ggatgtatgg gtagtcgatg cctcgcggtt atcggtagaa gaaattcgct caatcgcaga
     6601 gcggaacaaa caggaaaatc caaacctgtc actcatcatg gcggattatc ttggcctgat
     6661 tgagaagccg aaagcagacc gcaacgacct cgcaattgct cacatctcag gaagcctgaa
     6721 ggcgatggcg aaagacctga aaacgcctgt tatctcccta agtcagcttt cgcgcgatgt
     6781 tgagaagcga ccaaacaaac gcccgacaaa cgcagatttg cgtgattcag gaagcattga
     6841 gcaggacgca gactcaatca tcatgctcta tcgggaagcg gtatatgacg agaacagtag
     6901 cgccgcacca tttgctgaaa tcattgtgac gaaaaaccgg tttggcccac ttggtacggt
     6961 ttaccagcgg ttctgtaacg gacactttgt tgcatgtgac caggatgaag ccagacagat
     7021 ttgcacagca tcaaatgcac ctgcttcgcg tggcagacga tatgcacaag gggctgacgt
     7081 atgaccatct acatcactga gctaataaca ggcctgctgg taatcgcagg cctttttatt
     7141 tgggggagag ggaattggag gctttaagaa atgagtacga tagctgagct tgtcagggct
     7201 aattttcgtg aagagttggt gcgttggtat cggtatcgtt catcgtccag tttgccgatt
     7261 gatgagttgt atgagcattc acctgctgca cgacgctatc cgcgtgaccg tgtccttcga
     7321 cggttgttca aactcaacaa tgagtttcag cgcaacagaa taatccggag tctggattta
     7381 aagtgaagga gtgagcatga gtcgaagaag tagctttttg gggtttgtaa tattcctgtc
     7441 ctgcactggt tacatcgtaa tctggtcaat ttcgaacatt gaccgtggcg gggaatatct
     7501 cattgtaatg ttctttcctt tgtttcttgg gtggtacgcc gcaaggttgc tggaagaatg
     7561 gggttacagg cataaaaaat aaaggagtgt tcagtgaagc aaaccctttt ttattggatt
     7621 aagagcgagg ggtaagtacc gatggtaaat gcatttattt gtagtttatt tcttgtcgcg
     7681 atttttcatg gattccttct gatgatgagt tttgttctct ggaataatgg atatcgcata
     7741 ttgggagtag gttttgtttt acggttttca gttgtctgcg cgttgctacc gataattatg
     7801 gcgactatca aatattattg gtaacccaaa aaatcatcga tggagagtga
//
LOCUS       DAZGJW010000194         7703 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZGJW010000194 DAZGJW010000000
VERSION     DAZGJW010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.194"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            446..1453
                     /locus_tag="ACHKIK_004518"
                     /pseudo
     CDS             446..1453
                     /locus_tag="ACHKIK_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2073..3582
                     /locus_tag="ACHKIK_004519"
                     /pseudo
     CDS             2073..3582
                     /locus_tag="ACHKIK_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3633..3747)
                     /locus_tag="ACHKIK_004520"
                     /pseudo
     CDS             complement(3633..3747)
                     /locus_tag="ACHKIK_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3834..4310
                     /locus_tag="ACHKIK_004521"
     CDS             3834..4310
                     /locus_tag="ACHKIK_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9085552.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACMAQIARENGVND
                     DVIFKWLRLWQNEGRISRRLPVTTTSDAGVELLPVEITPDEPKEPMAALTPSLSTQTT
                     VSASSCKVEFRHGNMTLENPSPELLTVKWHTEFGHLNRGDMLTSEQHRCSNEKKKF"
     gene            4291..5443
                     /locus_tag="ACHKIK_004522"
                     /pseudo
     CDS             4291..5443
                     /locus_tag="ACHKIK_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
     gene            5506..6955
                     /locus_tag="ACHKIK_004523"
                     /pseudo
     CDS             5506..6955
                     /locus_tag="ACHKIK_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7022..7514)
                     /locus_tag="ACHKIK_004524"
                     /pseudo
     CDS             complement(7022..7514)
                     /locus_tag="ACHKIK_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558155.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            7437..7667
                     /locus_tag="ACHKIK_004525"
     CDS             7437..7667
                     /locus_tag="ACHKIK_004525"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO9085553.1"
                     /translation="MNCWRCFPLFFIGILTTDEWGSHIRYVIEGDSSIFIQHIECMRI
                     LIKHLTRRAICFPHSVNFIKKHRYFIEKYMFY"
BASE COUNT         2041 a         1831 c         2047 g         1784 t
ORIGIN      
        1 gggtgttttt ttaatctttt tgggatttaa attcctatcg atcgagtagg cagcctagcg
       61 gctgcggctt gtcatggcct gaaattaccg ttataaaaac aaacaatgtc attgtctttc
      121 aggtagttat atggcacgtt cagctaaacc ccgtaaacga aaactcgcac ctcaaagaag
      181 caaacttccc cgctatgttg taaaacttca cgaagatgat ttttttgacg aagaagaagt
      241 agagattctg cgcttcgata attttgacga tactgttgag tgttgcgcag acctgaatat
      301 tccgttcttt gtggatgccg gaaacaaaaa gctggtcttc tggtttgtac gtgttgatga
      361 cgaagggcat ccggaaatcg cccgctgtac agagcgggag tttacgacca ttcttgccgg
      421 tatcagcgcc ggcggcatgt actgcccgga ctgttgcaat acaaagaagg tctgttttcg
      481 gtgtaaaaaa cgctcctgcc cacactgcgg ggtgaaagcg ggtgcacagt ggatacagta
      541 tctgctgagt ctggtccccg acggcccgtg gcagcaattg tattcacgct tccctgccag
      601 tactggtccc tggtgttcca caaccggtgg ttactggcag agatgagccg catagcagca
      661 gatgtgaagc cggggatatt cacggtaatc cacacatggg gccgggacca gcagtgtcat
      721 ccacatatcc acatatccat ttatccattt atcgacaact gccggcggtg tgacatcaca
      781 tcacacctgg aaaaaccttc atttttacgc ccgtaaggtg atgagtatgt agcgataccg
      841 gataacacgg ttactgtcac ggaaataccc ggaactattg ataccggatg agctggctgc
      901 tgaaggaagc agtaaacggg aatggaatcg tttcctggac agtcattacc gacggggcag
      961 gaatgtcaac gtatcaaagg tgatggataa cgccacacat gtggcggtgt actttggctc
     1021 ttacctgaaa aagccgccgg tgccaatgag ccgtctggag cactatgccg gtcaggatga
     1081 aatcggactg cgttacaaca gtcaccgcac acgacgtgaa gaatacctat tgatgagtgg
     1141 tgatgcgttt atggaaaggt tctcctggca tgtggcggat aaggggttcc gtatggtgag
     1201 gttctacggg ttccttagtc cggcgaaacg ccggttgctg gaagaattag tgtacgtaat
     1261 aacagagacg gtgataaaaa cggcgatgca aatcaggtgg agagggatgt atcagcggtt
     1321 actgaaggtt gacccgctga aatgcatcct gtgcgaaagt cagatgcggt ttacggggct
     1381 gaagcggggt taccgtctgg cagagctggt tatgatgcat gagccgctgg cccgaatgca
     1441 gtattgcggc tgagagccgc agaggggaag ttgcgtccat tttgagggaa atggagcaaa
     1501 aaaacgtcac cgataccctg tatcaatcaa tgtcagcaat ataagcgctg atatataccc
     1561 gatgaggttc cggatgtgtt gaataaatgt tcgttttttg ttttcttaat ctttccgggg
     1621 gataaattcc tgtcgatgaa catatcaaaa tcaggaaatt acacgaatct atgtacaggc
     1681 tctctgggaa cactttacgc tttgacacag agggctaaat aatgagcgat tcagatgcca
     1741 tttttgcata cagaggattg acagcattcc catgctaatt gttaaggttt aaattgaagt
     1801 atatctatct gtcatttttg caaatgacat gcggtagcat aacgttccga ggtctcttat
     1861 gcagacatac gtggcgacat taaccactcc cagatacagc cagccttcat ccgtacgtaa
     1921 gtacgtgatg tctcgcgccc acttctggct ctggctactg gcgtaaaaat cctgctcctg
     1981 tcacgaacgg tgcaaaagtg gttcttccgc aacctgggtg gagaaaatgg atgaaacttt
     2041 atggtgaaca aggatctaag ctgtttttag ttatgggtaa cgcaatgcac tctcttaaaa
     2101 cacttctaca gttaccttgc ggatggcgat gtagtcgaca aattattaac tctgacggta
     2161 tacccctcca tctccgcgga aaacgcaaaa cagcacaatg tcctgaatgc tttaagcgta
     2221 gcgactctgt tcatagttgt cgtcggcgcc ggatacagca tctaccctgc tcagggcaga
     2281 cgctatggct tgtattttcc gtccgccaca ggtacagccg taacccggct tgttcacgaa
     2341 aaatttttgc cgagtcgttt gttcccttcg gtggttcaca tctgcagtct tcacaggcgt
     2401 tacaaaattt acagcgtcaa ctgggattaa tcgccggagg tgaggccgga aaacgggcag
     2461 caacggcagt gggtctccgt tgcagtgctg atactctcct tcgcagggtt atcaataccc
     2521 cggagacgaa acagtcaggc gcgcctcatg tcggtattga tgagtgggcg tggcatcggg
     2581 gccaccgtta cggtacgtta atcgtcaatc ttgatactca ccgtcccctc gtcctgcttc
     2641 ccggccgtga tcagcgtacg ctggcgacct ggttcagaaa atatccggaa atacagattg
     2701 tctcgcgtga tcgcagtggg gtctgtgcga cagcagcacg tgaaggtgta ccacaggcca
     2761 gacaggtggt cgatgccacc tgctaaaaaa tattggcgat gcgcttgaac gaatgatgta
     2821 cagacatata cctctgatac gtcttgttgc cagcgagttg tcaccaaaga aaccacctga
     2881 gccagaactg tctgagcctg cagcatcgct ccgtcgtccg gaacgcctaa aacaacaaac
     2941 ccgcaaaaaa cggcatcagc gttggacaga ggttatggcc cagcataaca agggatgtag
     3001 tttcagggaa atatcccgta ttacaggcct gtcgcgtgtg acagtcagtc gctgggtgcg
     3061 ttcaggaaca ttccctgaaa tgtcaacccg acctccaaag cgagggcttc tggacccatg
     3121 gagggagtgg ttaaaagagc aacgagaaag tggtaattat aacgccagcc ggatatggcg
     3181 ggaaatggtg gcccgggtaa gccgctggtt gatgccctgg agaataacca gggatgaaga
     3241 aaattatgct tcccgattta ttagtctgat gtgtgaaaag aacctgagct gaaaatagcg
     3301 cagcaactgg cactcgagtt ctaccgtatt ctgaaaaccc aaaataaatc acagctaagt
     3361 agttggttca ctcgagtcca cgaaagcggt tcagcagaat ttcggcgagt ggcggcgggg
     3421 atggaagctg atgctgcagc tatatgtgag gcaatcagta gtcgctggag taatgttgtt
     3481 gtcgaaggtc atgtaaatcg cctgaagatg ctgaaacgcc agatgtatgg tcgagcagga
     3541 tttgaactac tcaggcagag ggtaatgagt ccactggcat gaaaatctcc acccaggttg
     3601 cggaagaggc acttttcatc cgtcgttgac agctccagca gattttctga cacaggcagg
     3661 ctgtgtgcgg taagggctga acttccggga agcctttgcc ctcagtccgt gacggcacag
     3721 gcttgccatc acggttttta cgttaaaagt aagcgtaaac cgaccgcagt atgtagccat
     3781 tagacaagaa ttggtaactt agacgcccat ctgacacaga cggacatcca agtatggaat
     3841 tacaggactg gcgaaaagaa cctcgtaaaa agtattcgaa tgaattcaaa cttcgtatgg
     3901 ttgaactggc atcacaaccc ggtgcttgta tggcccagat tgcacgagaa aatggcgtca
     3961 atgatgatgt tattttcaaa tggctcagac tctggcaaaa cgaagggcgt atatcacggc
     4021 gtcttccggt gacaaccact tctgacgcag gcgttgaatt attacctgta gagataacgc
     4081 ctgatgagcc gaaagaaccg atggctgctc ttactccatc cttatccact cagactactg
     4141 tcagtgccag ctcctgcaaa gtggagttcc gtcacggtaa catgacgctg gaaaatcctt
     4201 caccagagct gctcactgtg aagtggcaca ctgaatttgg ccacctgaac agaggtgata
     4261 tgctcacctc agaacaacac aggtgctcta atgaaaaaaa gaaattttag cgcagagttt
     4321 aaacgcgaat ccgctcaact ggttgttgac cagaaataca cggtggcaga tgccgccaaa
     4381 gctatggatg ttggcctttc tacaatgaca agatgggtca aacaactgcg tgatgagcgt
     4441 cagggcaaaa caccaaaagc ctctccgata acaccagaac aaatcgaaat acgtaagctg
     4501 aggaaaaagc tacaacgcat tgaaatggag aatgaaatat taaaaaaggc taccgcgctc
     4561 ttgatgtcag actccctgaa cagttctcga taatcgggaa actcagagcg cattatcctg
     4621 tggtcacact ctgccatgtg ttcggggttc atcgcagcag ctacagatac tggaaaaacc
     4681 gtcctgaaaa accagacggc agacgggctg tattacgcag tcaggtactt gagctacatg
     4741 gcatcagcca cggttcggcc ggagcaagaa gcatcgccac aatggcaacc cggagaggct
     4801 accagatggg acgctggctt gctggcaggc tcatgaaaga gctggggctg gtcagctgtc
     4861 agcagccgac tcaccggtat aaacgtggtg gtcatgaaca tgttgctatc cctaactacc
     4921 ttgaaaggca gttcgccgtg accgagccaa atcaggtgtg gtgcggtgat gtgacctata
     4981 tctggacggg taagcgctgg gcgtacctcg ccgttgttct cgacctgttc gcaagaaaac
     5041 cagtgggctg ggccatgtcg ttctcgccgg acagcaggct caccatgaaa gcgcgggaaa
     5101 tggcatggga aacccgtggt aagcccggcg gggtgatgtt ccacagcgat cagggcagtc
     5161 attatacgag caggcagttc cggcagttat tgtggcgata ccagatcaga cagagtatga
     5221 gccggcgcgg aaactgctgg gataacagcc caatggaacg cttcttcagg agtctgaaga
     5281 gaacgaatgg atgccggtgg tgggttacgt aagcttcagc gaggcagctc acgccataac
     5341 ggactatatc gttggatatt acagcgcact aagaccgcac gaatataacg gtgggttacc
     5401 cccaaacgaa tcggaaaatc gatactggaa aaactctaac taggtggcca gtttttgttg
     5461 accacttcag aaagccagtt ccgtcagaaa gaccagcaac taagtctggt tgaagagacg
     5521 gaggccttcc tgtgctctgc actggcacgt gccgaagaaa agatcgaaga agatgaacgg
     5581 gaaatagaac atctgcagac tcagatagaa aaactgcgcc ggatgctgtt cggtacccgt
     5641 tctgaaaaac tgcgtcgtga agttgaacag gctgaggccc tgctgaaaca acgcgaacag
     5701 gacagcgatc gttacagtgg gcgggaagac gctccacagg ttccccgcca gttgagacag
     5761 ccgcgccatc gtcgtccgtt accggcacac cttccccatg caatacaccg tctggagtct
     5821 gaagaaagct gttgcccgga gtgtggcggt gagctggatt atctggagga agtcagtgca
     5881 gaacaactgg aactggtgag cagcgccctg aaagtgatcc gcactgttcg ggtaaaaaag
     5941 cctgtacaaa atgtgactgc atcgttgaag caccggcgcc gtcccgcccg atagagcgtg
     6001 gtatcgcggg ccccggatta cttgcccgcg tgttaacggg aaaatactgc gaacagctgc
     6061 cactgtatcg tcagagtgaa atctttgccc gacagggtgt cgaactgagc cgtgcattac
     6121 tctccaactg ggttgacgcg tgctgccagt taatgactcc gctgaatgat gccctgtacc
     6181 gttatgtgat gaatacccgc aagcttcaca ctgacgacac accggtaaag gtactggcac
     6241 tgggcctgaa aagacgaaaa cagggcgcat ctggacgtat gtccggaatg atcgcaatgc
     6301 gggttcgtca tctcctccgg cggtctggtt cgcgtactca ccgaaccggc aggggaaaca
     6361 cccggagcaa cacctccgcc ccctgcaggc ggatgcgttc aacggttacg atcggctgtt
     6421 cagtgcagaa cgtgaagatg gtgcgctgac agaagttgcg tgctgagccc atgtccggag
     6481 aaaaatccac gatgtataca tcagcagcaa aagtgcgacg gcagaagaag ccctgaagcg
     6541 catcagtgaa ctgtacgcca tcgaggatga aatacgggga ttaccagagt cagagcgtct
     6601 tgaagtcagg cagcagcgaa gcaaagcgtt actgacgtcg ctgcatgaat ggatggtgga
     6661 gaagaatggc acgctgtcga aaaaatccag accaggcgaa gcgttcagct atgtactgaa
     6721 tcagtgggac gccctctgtt attacagtga tgacggtctg gcggaggcgg acaataacac
     6781 agcggaaaga gcgcttcgtg ccgtctgtct cgggaaaaag aattacatgt tcttcggtag
     6841 cgatcacggc ggcgagcgtg gagcactgct gtacgggctg atcggcacct gccgtctgaa
     6901 cggtatcgat ccggaagcgt atctgcgcca tattctgagc gtactaccgg aatagccctc
     6961 caaccgtgtt gacgaactcc tgccatggaa cgtagtactc accaataaat aagcgtcaat
     7021 acggtgctcc gtagcgctgt tttgaccgga taaaagccgc gagtaccacg ctgtcgcagt
     7081 gctgacggaa ctgctgacat gtacttatcc ttgtccgccg ctgacaccac atataccagt
     7141 cactgcggcc ttcctggagc acatggcaca ttgccttgat gttgaacaca gcctgatgtt
     7201 tttcaataaa gacatacttc atttcaggcg cttcgcgaag tatgtcgcgg ccttttggag
     7261 gatggtcagc tcttcatccc gttccgccag ctggcgtttg agacgtgcaa catcggtaga
     7321 catctccagc tcacgttcag aagaacgtct actgattttg ttgttgacta cccagttgta
     7381 gactgtgatt catacaggct gagctcacgg gctgcagcag caatacctat actgttgtga
     7441 attgctggcg ctgcttccct ctttttttca ttggtatatt gaccactgac gaatggggta
     7501 gtcacatcag atatgttata gaaggagact catctatttt tattcagcat attgagtgca
     7561 tgagaatatt aatcaaacat ctgacccgta gggctatctg tttcccgcac tctgtaaatt
     7621 tcataaaaaa acaccggtac tttatcgaaa aatacatgtt ctactaattg gatatcacca
     7681 aatcatataa aataatgtgt ttt
//
LOCUS       DAZGJW010000195         7617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZGJW010000195 DAZGJW010000000
VERSION     DAZGJW010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.195"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            17..745
                     /gene="gadW"
                     /locus_tag="ACHKIK_004526"
     CDS             17..745
                     /gene="gadW"
                     /locus_tag="ACHKIK_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO9085554.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1108..4221)
                     /gene="mdtF"
                     /locus_tag="ACHKIK_004527"
     CDS             complement(1108..4221)
                     /gene="mdtF"
                     /locus_tag="ACHKIK_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO9085555.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4246..5355)
                     /gene="mdtE"
                     /locus_tag="ACHKIK_004528"
     CDS             complement(4246..5355)
                     /gene="mdtE"
                     /locus_tag="ACHKIK_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO9085556.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5463..5741
                     /locus_tag="ACHKIK_004529"
     CDS             5463..5741
                     /locus_tag="ACHKIK_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085557.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5742..6269)
                     /gene="gadE"
                     /locus_tag="ACHKIK_004530"
     CDS             complement(5742..6269)
                     /gene="gadE"
                     /locus_tag="ACHKIK_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO9085558.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(7068..7616)
                     /gene="hdeD"
                     /locus_tag="ACHKIK_004531"
     CDS             complement(7068..7616)
                     /gene="hdeD"
                     /locus_tag="ACHKIK_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013368005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO9085559.1"
                     /translation="MLKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFVSGDILSTV
                     VGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPELGIFAIAAF
                     IAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVSVTLVSTLV
                     GIELIFSAASLFSFASLFVKQQ"
BASE COUNT         2001 a         1835 c         1809 g         1972 t
ORIGIN      
        1 aatataaggg ataatcatga ctcatgtctg ctcggtgatc ctcattcgtc gttcattcga
       61 tatttatcat gaacagcata aaatatcgct gcataacgag agtatcgtgc tgctggagaa
      121 aaatttggca gacgattttg cgttttgttc accggatacg cgacgactgg atatcgatga
      181 gctgacagtt tgccattact tacaaaatat tcgtcagcta ccacgcaatt tagggttaca
      241 tagcaaagac cgtctattaa ttaaccagtc accccccatg ccgctggtga cggcgatttt
      301 tgatagcttc aatgaatccg gggtaaattc accgatactg agcaatatgc tctacctttc
      361 ctgtttatcg atgttttctc ataagaaaga actgatcccc ttacttttca atagcatcag
      421 cactgtttca ggaaaagttg aacgccttat tagctttgat atcgccaaac gttggtatct
      481 gcgcgatatc gcggaaagaa tgtataccag cgagagtctc atcaaaaaaa agttgcagga
      541 tgaaaatacc tgtttcagta aaatattact cgcttccagg atgtcgatgg ccagacgatt
      601 actcgagtta cgtcaaattc ctctgcatac tattgcagaa aaatgtggct atagcagtac
      661 gtcgtacttt ataaacacat ttcgacaata ttatggtgta acgccacatc agtttgcgca
      721 acattcgcca ggtacctttt cctgacatat tttgcatttg aatattggtc aggatctcac
      781 acctgcttca tgtgaaactc ttccctgatg atttctgccg ggctaccggc tagttctctt
      841 tcgcagtttg ccaggcggta taggtatttt caggaggaaa agactcggca tgtttgggat
      901 tattaagctg acaattcata cgattacggg aatgtgggtc agtattgtgc tgttcaaatt
      961 gatgacgaat ggctggagtg gtttctattt tcagtgctgt gtactaagcc tggtattcct
     1021 gacggttagc tggttgttga gtggtgaatg gttagcagga aagagtaagg ctgaaccttc
     1081 acgttcaacc ttactctcat ttacacgtta cgctttttta aagcgggcaa agagatgttc
     1141 cactacaacg aaaaagaccg gaacgaagta aattgccagc actgttgcgg caaacatccc
     1201 gcccatcacg ccggtaccta ccgcgttttg cgcgccagaa ccggcaccat gactgataac
     1261 cagcggcagc acgccgagaa taaaggccag agaggtcatc aggattgggc gtaaacgcat
     1321 ccgcgccgct tcgatgattg cctctatcgg cgttttccct tctttctgca tcatctcaac
     1381 ggcaaattcg acaatcagga tggcgttctt ggcggaaagc ccgatggtgg tcagcaaacc
     1441 aacctggaag tagacgtcat tacttaagcc gcgcagatcg gtggccagta atgcgccaac
     1501 gacgcctaac ggaacaacca acatcaccga gaacggaatt gaccagctct catagagtgc
     1561 ggcgagggcg aggaacacca cgaccagtga aatggcatac agtgcaggag cctggtttga
     1621 ggataacgct tcctgatacg acagtccggt ccatgagtag ccgacgcctg ccggaagttt
     1681 agcgaccagg tctgccataa atttcatagc gtcaccggta cttttcccgg ccgccgcttc
     1741 acctaaaatc tccattgacg ggatgccgtt gtagcgttcc aatcgcggtg aaccataggt
     1801 ccattcggta gacgagtagg cagaaagcgg tgccatcgtg ccagaggcgt tgcgtacata
     1861 ccattggttg atgttatccg gcaacatacg gaacggcgtg cctgcctgga catacacttt
     1921 tttcacccgc ccctggttga ggaagtcgtt cacgtagctg ctgccgaagg cggtggaaat
     1981 tgtctggttg atatcagaca gcgccacacc cattgcctca gctttcgcgg cgttgacgtt
     2041 cactttgaac atcggcgtat cttccaggcc gttcgggcgt accccggtga cctggtcagg
     2101 cgattgcgct gccagtgata acagctcgtt tcgtgcctgg gttagttttt cgtgccccag
     2161 gttaccgttg tccagcagtt ccatatcaaa acctgacgcg gtacccagtt cagccaccgc
     2221 gggtaagttg aacgggaaga cgacggcttt attgatactg cttaacgcaa tcattgcccg
     2281 ctgaatgatc gcggtaaccg agttttcctc accgacacgt tcagaccacg gcttgagact
     2341 gataaacgcc aggccgttgt tttgcccctg gccgctgaag ccaaagccgc caacggtaaa
     2401 caccgactga acattatctt tctcttttgt aagataataa tccgtcacct gttgcagcac
     2461 tttcgtggtg ttaaccatgg tggcaccgga aggtaactgc gcggtggtca taaatacccc
     2521 ctgatcctct tctggtaaga aagaggtcgg tgtacgcagg aacagcaccg ccatcccggc
     2581 gcaaatcagc aggtagacca ccatgtagcg accggtacaa cgcaacagcg agcgggtgct
     2641 atcggtatag tgctgagttg atttttcaaa cagcgtgttg aagcgtgcga acagggcgtt
     2701 aggtttgtga ccgccttccg gcgcggcttt cagaatggtg gcgcacaggg caggggtcag
     2761 gctcattgcc acaaatactg aaagcagcat ggaggagatc agcgtgatgg agaactggcg
     2821 gtagatctcc ccggttgcgc cgctcataaa ggccatcggc ataaacactg cggaaagaac
     2881 aacagcaata ccgaccagcg cacgttggat ctgccccatc gatttatgcg tcgcttcctt
     2941 cggtggtagc ttatcttccg caatgacacg ctcgacgttc tccaccacca cgatggcgtc
     3001 atccaccagt aaccctatcg ccagcaccat cccgaacata gttaacgtgt tgatggtgaa
     3061 accgaccgcc gacaagatcg caaacgtccc gagaataacc accggtacgg caatcgtcgg
     3121 gatgattgtg gcacggaaat tctgcaaaaa cagatacatg accaggaaga cgaggatgat
     3181 agcctcaacc agtgttttaa aaacttcctg aatagaaatt tcgataaacg gcgtggtgtc
     3241 gtaaggataa accgtcttca gacttgcggg gaaataggct gataagcggt tcagctcctc
     3301 tttgactgcc cgcgaggtat ccagggcgtt tgctccggta gccagtttga tggcgatccc
     3361 ggcagcaggt ttgccgttat agcgcgccac ggtggaataa tcttccgctc caagttcgac
     3421 gcgggcgaca tcacgcagca gcacttgcga accatcttgc tgaactttca acaggatttt
     3481 gccaaattct tccggcgttt gcagacgcgt ctgcacaatg atcgaggcgt ttagttgctg
     3541 gtctgccgcc tgtggcatgc cacccagttg accaccggaa atctggttgt tttgcacctt
     3601 aatctgggaa ataacatcgg aaggtaccag attgtattta ttgagttttt gcgggtccag
     3661 ccagatacgc atggcgtact cggaaccaaa gagctgtacg ctaccaacgc ccgcggtacg
     3721 gcttagcggg tctttgatat tagacgctac atagtccgcg atatcgtact ggttgaggct
     3781 gccgttatca gaaataaacg ccgctaccat cagaatatta ctgctcgact tatcaacgct
     3841 aatcccctgc tgctgcaccg cttcaggtaa tgaaggcata gcgagttgca gtttattttg
     3901 cacctgaacc tgtgcgatat caggagatgt cccagtctcg aaggtcagag tgatagaggc
     3961 attacccgcc gcatcactgg ttgaagacat gtacatcagg ccatcaagcc cattcatatt
     4021 ttgctcaatc acctgagtga ccgagtcttc taccgtttgc gcatcggcac ctggataggt
     4081 agcgctgacg gtaatggtcg gtggcgcaat ctgcggatac tgcgcaaccg gtaagttcat
     4141 gatcgccaga ccacctgcaa gcatcataat aatggcaagt acccaggcaa aaaccgggcg
     4201 atcaataaaa tagttagcca tgaaagtccc cttaagcctg caacgttatt gtttcgattc
     4261 ggtgctggcg ttttcctggc tggaggaaat tgctcgtgct ttgatacccg gacgaatgcg
     4321 ttgcaaaccg gaaacgatca cccgatcgcc agcctgcaag ccagaggtga caacccactg
     4381 gtcgccgatg gctttgctgg cttcaatttc gcgtagctgc acaacatcgt ctttatccag
     4441 aatgagcgcc gttgctttac cctgggcgtt gtgggtgacg ccttcctgcg gcaccagtaa
     4501 tacattctgg cggctacctt catccactaa tgccgtgacg tacatgccag gcagcaagtc
     4561 accatttggg ttggggaaaa tcgcccgtaa cgtcacggag cccgtggttt catccaccgt
     4621 cgggtcggag aatttcagcg tgccggtctg gctgtagcgt ttaccatttt ccagattgag
     4681 ctgtactggc gtactgccct gaacctgttt gatttgccca ctggcgacct cttctttcat
     4741 gcgtaagaaa tcttgcaccg actgcgtgag atcgacataa atcgggtcca gacgttgtac
     4801 ggtaaccagc gaatctgcct gattagcggt aacgagtgcg ccgacggtca ccgacgattt
     4861 cccgctgacg cccgtaatcg gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg
     4921 ttcaacagcc gctttggcga cggtgacatt ggcttctgct tcattcaact gggtgcgcgc
     4981 ggtatcgtaa tcctgacggc taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa
     5041 ggtgatgcgg gcattgctgg cggtagagag cgctttcgcc agcgagcctt tggcggagtt
     5101 tagctcggcc tgtaaaggtg caggatcaat ctgatacagc gaatcgccct ggttcacttt
     5161 atcgccttcg ataaagttgc gtttaatgat aataccgccc acctggggac gtatctcggc
     5221 aacttcataa ggaacggttc taccgggcaa ttcgctcaac acattgaccg aaccggggga
     5281 gagtgtgacg acaccgacct caggcgtcat ggcggcggcg ttttccgccg atttgtcatc
     5341 gcaggcggtg agcatcgcgc cgcagaataa caacggtatt aacagctttc ttcttctgtt
     5401 cattttagtc cctgaaaatt cttgagaaat aacggttctt taaattgcgc gcgaggagag
     5461 atatgaggga tttacagacc agcggcatag tggggttgtc agccagtaag gtgggataca
     5521 ggcacagtga tcgacatggt gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag
     5581 ttcctgccag cattcgggca acgaagggtt ctactggtgg atacacatac caggggaata
     5641 aagtcgtttt agcctctcct ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc
     5701 ctcgtcatgc cagccatcaa tttcagttgc ttatgtcctg actaaaaata agatgtgata
     5761 cccagggtga cgatgtcgct catacgtctt agtttcagct tgtacataat gttgaatttc
     5821 tgaatcttta atgtttgttg cgaaatattg agcgtcgacg tgatattgct ttcattttta
     5881 tttttcagaa tcagttgcat aattttgaac tgattatgtg aaaaacataa ttgtgaattc
     5941 ttatggggca agtgtttacc ataagtaatc tcaagaagat ttttgatact ttctttgcgg
     6001 ctaacaaaat aaataggcgc tttagctttt agtttactga tgtgataagg tgccaaaaca
     6061 ataattctct cggcatctaa tttctccaga aatttaatcg cttcttcatc aaggatatga
     6121 ttgtgatagg tatcaatgat aaccttgaag ggtttattgc ctactttttt gatctctgac
     6181 agggaattga tttttatcat ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga
     6241 aaagaatctt tcgtcatgag aaaaatcata acttgctcct tagccgttat cgtttcgaac
     6301 atgtcatcct tttcattatt tacatccttg tccgaatcgt tgttcaatat agtaaacgcc
     6361 tataagaact ttgcccttcg tgaactaata cgtctattaa cactaatcaa tacttcagat
     6421 ttatccatca cgcactccat atattgatcc taagtaagat tcctggttgt tatcaatttg
     6481 taaatatcaa acaaaaagtt aacgtttgcg gattactggc caagaataag ttatcaataa
     6541 ccagtttgtg atctctgaag aatattacta aagttaaaat gtaatccgat ttaaatatcg
     6601 agtctccttg tttcgactta agctggcaat tggattgcca gctttctttt aacgcattga
     6661 aattacctga gtaaataacg cattcatgtc ttcaggtttt tatatagcgt ctggagacac
     6721 ggatatttat gcaatgtaat caacgccagt tgcaaaaaaa tgaacccgga ttacttattt
     6781 aaccgcccat agcatcaacg cttacggaac tctttgttca ctttcccaca gcacttttaa
     6841 aacattaaac ctgactacgg aaaatatcag ccatgataat gtttgaaatg gctaatttgc
     6901 catggagtga aaaaaattag atgaaattca gtaggttgaa atgatcacta gcaggtaatt
     6961 atttcaatga tagtgcgcaa ttgatctaca acactgcgta gcggagagag tattaatcgg
     7021 atcatagtca catcaagtga ctatgatcct ggtgacaacc gggataatta ttgctgctta
     7081 acgaacaaac tggcgaagct gaacaggctg gcggcgctaa atatcagttc aattcccacc
     7141 agtgtggaaa ccagcgttac agacaccatc ggcgttgcac caaggaatat ccaggcaatg
     7201 acgatatcca gcacaccaat aacgagctgt agccagctgc ctttcattga acgctgacga
     7261 taccaactca tcaggcgaat aacccctgca acacagaaca aaccggcaat aaatgccgca
     7321 atggcaaaaa tgcccagctc cggtgcgcgg atgaagaaat agccgatcaa taaataggcg
     7381 actgcgacga ggaaaccgga taataccggc cagaaattat gactgcggtt gctgaataac
     7441 ccgacaataa gcgcaatacc cgagcagatt aataatgcac ccactactgt gcttaaaata
     7501 tcgccagaga cgaacgggaa actgatacac agcaacccga cgataaacag cagcacggca
     7561 ataaactgga ttgctctgcg atgctttttc agcatctcca gatcaaactt caaaatt
//
LOCUS       DAZGJW010000196         7502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZGJW010000196 DAZGJW010000000
VERSION     DAZGJW010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.196"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(239..601)
                     /gene="yibL"
                     /locus_tag="ACHKIK_004532"
     CDS             complement(239..601)
                     /gene="yibL"
                     /locus_tag="ACHKIK_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO9085560.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            886..1095
                     /gene="yibT"
                     /locus_tag="ACHKIK_004533"
     CDS             886..1095
                     /gene="yibT"
                     /locus_tag="ACHKIK_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO9085561.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            complement(1107..1694)
                     /gene="mtlR"
                     /locus_tag="ACHKIK_004534"
     CDS             complement(1107..1694)
                     /gene="mtlR"
                     /locus_tag="ACHKIK_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO9085562.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(1694..2842)
                     /gene="mtlD"
                     /locus_tag="ACHKIK_004535"
     CDS             complement(1694..2842)
                     /gene="mtlD"
                     /locus_tag="ACHKIK_004535"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO9085563.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            complement(3072..4985)
                     /gene="mtlA"
                     /locus_tag="ACHKIK_004536"
     CDS             complement(3072..4985)
                     /gene="mtlA"
                     /locus_tag="ACHKIK_004536"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO9085564.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSTILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGEYIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            5522..5884
                     /gene="yibI"
                     /locus_tag="ACHKIK_004537"
     CDS             5522..5884
                     /gene="yibI"
                     /locus_tag="ACHKIK_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO9085565.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            5887..6953
                     /gene="yibH"
                     /locus_tag="ACHKIK_004538"
                     /pseudo
     CDS             5887..6953
                     /gene="yibH"
                     /locus_tag="ACHKIK_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(7413..>7502)
                     /locus_tag="ACHKIK_004539"
     CDS             complement(7413..>7502)
                     /locus_tag="ACHKIK_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HFO9085566.1"
                     /translation="NICSDDKNIKLRVNSTIMAIDDSIKCIDE"
BASE COUNT         1852 a         1863 c         1836 g         1951 t
ORIGIN      
        1 ccgaaaaaaa gagacgtaaa tgcttgttaa ttaataaatg atatcttcta tatattttta
       61 ttgtttatag gtggctattt cggtaaaaat gcattttcag aatatcaacc agcggtaaat
      121 cgacgtaaag ggagtgaatt gtttggtaat taggcgagta gacaggagag tccccgcatt
      181 gaccagctaa ggcaagccag atgcgggttg atatgatcga accagtcgtc tgaggcgctt
      241 aaaacgcagt cttagagaat ccagtcatct cctgcaggcc catttcacgg cccagcgcgg
      301 tcattgggtg cacaaccacc agtccgcgaa cacttttctt cagcttgccc atatcagcct
      361 gctctttttt ggtaattgcg cgctgaaacg gcatcttcat cagcttctgt gcttctttac
      421 tcagtttttg gctatgaacc tcacgcaggc gagcaatttc tgcttccagc gtggctttct
      481 ctttttccag ctcggcatat ttgtctgcgg cttcaaccag cgacagatcg gcctgctggt
      541 ggcggatggc gtccaggcga tcgctgagac gtttgatttc gtttttttcg acttctttca
      601 tgtgatggct tctgataaag ataaatttat agcccctagt atgcgcgacc aggtgctttg
      661 ttcacaactg atgacattgt aatagttctg caacgtaatc attaacatgc tcaaaaacta
      721 cgcaattcgt tgattaatta agaaatctat agaaaaaaga taataaacag cagcggatga
      781 ttattgagga ttcagcacgt tttccttaat gtcatcttta ccgggaacct aaactaaaat
      841 tggcggccgc gcattcccgg cgcggggtaa tacggagata tcatcatggg caaattaggt
      901 gaaaacgttc cgcttcttat cgataaagcc gtagatttca tggcatcaag ccaggcgttc
      961 cgggagtatc tgaaaaaact tcctccccgt aacgcgattc cgtccggaat acccgatgaa
     1021 agcgtgccgt tatatctaca acgtctggag tattatcgtc ggctttatcg gccgaagcag
     1081 gtagaggggc agtaagcaga cgttgcttac ttttgaaagg ctttttttaa gctgattctg
     1141 gaaatcagct cagtcagtga aaggaccatt gttgatcgca cagcctgttg atagcgctga
     1201 atttgcattg catacaaact ggagtctgct ggttcaaact gtggcggcgg tggtaacgcc
     1261 gccacgcagt gcagttcacc aaagggtcca aggatctcgt cgtcggtaaa ggcgtactcg
     1321 ttgccgtcgt gatttagttc ttcacgcaat gccatcagca gctccgcatc ttcgtattcc
     1381 tggcggttaa tgacgcccaa cccgtaaatg agttttaaac gcacagaaag atcgcccagc
     1441 ggaccgtcgc cgtcgagtaa cggttctaca gcatacttca ccgcgtaatc gtctttgcgg
     1501 aatacctgaa gcaccagaag atttaccgcc tcggtcaaaa gctcgacggc ggtgatcaga
     1561 aagcttcgca cggttttgcc agcattcaga cgctcaagca cacggttttc aaaggcctgg
     1621 gtttgttcca ttgttgcctg catatctgac aatctgttat tcgggcgcag ggtgtcctgc
     1681 gcctggtcca ccattattgc attgctttat aagcggttac cgcctcggat acaacctcgc
     1741 tgttggcatc aagaccggaa atctgtgcca gcgccgcctg cggacctttg tcagcgatca
     1801 gtgctgccag ttcctgagcc tgcggatcat cttcactgcg gaagtgcatt gcaccagcaa
     1861 tcccctgaat cagattcttg tgcggaaggc tgtattccag cgtaccgagc agtggcttga
     1921 tcagacggtc gccagcactc agtttacgca gcggctgacg gcctacgcgc tctacatcat
     1981 ctttcagata cgggttctca aaacgaccga ggattttctg gatgtacgcc gcatgtttgt
     2041 ctgcgtcaaa gccgtagcgc ttgatcagta ccgcaccact ttcttccatc gcacctttta
     2101 ccaccgcgcg gattttctcg tcgagaatag cgtcacgaat ggtctgatga ccggccagtt
     2161 ttccgaggta cgcggttata gcatgacccg tgttcagggt gaagagttta cgttcgacaa
     2221 atgccatcag gttgtcggtt aactccatgc ctgggatgtt cggcagtgcg cctttgaact
     2281 gcgttttatc gacaatccat tcgctgaaag tttctaccgt cacttccagc ggatcgttag
     2341 ttgccgaagc cgaaggcggt acgatgcggt caacggcgga atcgacaaag ccaacgtgtt
     2401 cttctaccca cgctttggcg tcttccggca gggcgttcat cacatggcct ttcagctgcg
     2461 tggtaccgcg taccatgttt tcacaggcga tgatgttcag cggggattca ttaccttgtt
     2521 ctttgcgttt caccagccct ttggcgatag ccggagcaat acgttccagc acaaccgggc
     2581 caacggcggt agtgactaaa tcgacctgag caatcagatc aacgacatca tcaccaatgc
     2641 tgctgacagc attgacgccg gaaacggtat ctacctgctc ggtttcacca accacatgaa
     2701 cctgatagct atgacgggca ttcagggcat caagtaccac ctgattgaca tcggcaaacg
     2761 tcagttggat acccgcgtct gccagcagtt taccgataaa gccacgaccg atattacctg
     2821 cgccaaaatg taatgctttc atagtattaa ccttcatcaa tgtttttacc cgagagggct
     2881 ggggtgaggc ttttccctca cccaaaccct ctccgtgagg agaggggcag cgccggatgc
     2941 ggcgtaagcg ccttatccgg cctacaagaa cgtgcaaatt caacaaattg tgatgcatcc
     3001 tgtaggcctg ataagcgtag cgcatcaggc aattaacccc accttctcca tgtggagagg
     3061 gtgggattgg attacttacg acctgccagc agttccagca cttcatccac gctggtggtg
     3121 tgtgccagac gctcgatgac ggactcatca tccagtgcat tggtcaggct ggtgataacc
     3181 tgaatgtgct cgttgttacg ggctgcaata ccaatcacca gacgggcaat gtcatcttct
     3241 tcttcaccga agcgcacgcc ttccgggtac tggcagaaca ccacgcccgt tttcagtacg
     3301 cgatctttcg cttcaaccgt accgtgtggc accgcgatat actcacccag ataagtcggg
     3361 gtcagttttt cacgatccag catcgcctga acgtattccg gctcaacgta accgcctttc
     3421 accagctgct cgccagcaaa acgaatcgct tcttctttgg ttgctgcttt gcgaccgagg
     3481 aagatgttct ccgcgcccag tttgaacagg ttagcactgg aatcgtcaaa gctgtctttc
     3541 agactgtctt tcactttcac ttcgttttca gtatggcgct gggcagcaac gagacgttcg
     3601 gttaggctgg tgtacaggcc gctgtcgagg aagttggtca gcgaaatatg ctgcgcctgc
     3661 ggaacctggc gcatagcgcg ttcggtcagg tcacggtgag tgatgacgag gtccacatct
     3721 ggcggcaggt tgttgatcgc gctgttagtg accgaaatct gtgacagacc tgcatcctga
     3781 attttcttac gcagcacgcc tgcgcccatc gcacttgaac ccataccggc gtcacaggca
     3841 acgatgattt tacgtacgtg gctcaggtcg ttagtcacat cgcccgcaga cagcggagat
     3901 gtgcctttag actcagcttt catgtcctgc atacgacgag ttgctgcttc aatatcatct
     3961 tcttctttca ctttgctggt tttcagcaaa atagtagaga caacgaagga gacagccatc
     4021 gccgcacaca cacccgcgat gttagcgaaa taagcacctt ttggtgtcat cgccagtaca
     4081 gcaaggatag aacccggaga tgccggagaa accagaccac cgcccaggat agtcagcgtg
     4141 aacacgccag tcataccgcc gaggatgact gcgaggatca gacgcggatt catcagcaca
     4201 tacgggaagt agatttcgtg gataccaccc aggaagtgga tgattgccgc accgcccgca
     4261 gactgtttag cgctaccacg accaaagaac atgtacgcca gcagcacgcc catacctgga
     4321 cctgggttag cttcaatcag gaagaagatt gatttaccca gttcatggga ctgctgaata
     4381 cccagcggcg agaagatacc gtggttaatg gcattgttga ggaacaggat tttcgccggt
     4441 tcaacaaaga tagacgccag cggcagcatg tcatggacaa ccatgaagtt aacgcccgca
     4501 gccagcattt tggacagggc ttcaacaatc gggccaatgc cgaggaatgc cagaatagcg
     4561 aggatcatcc cgatgatgcc tgcggagaag ttattcacca gcatctcaaa accggatttg
     4621 atcttaccgt ctacccagcg gtcgaagtgc ttaatgcacc agccgcccag cggacctgca
     4681 atcatagaac cgaggaacat cggcatgtct gcgccgacga taacgcccat ggtggtgatg
     4741 gcaccgacta cgccgccacg ttcgccgcct accagcttac caccggtata accgatcagc
     4801 agcggcagga gataagtgat catcggcccg accagcttcg ccagcgtctc gttcggtaac
     4861 caccctgttg gaataaataa cgcggtgatg ataccccacg cgataaacgc gccgatattt
     4921 ggcatcacca tgttgctgag gaaacgacca aagctttgca ctttgatctt aatatcggat
     4981 gacataaaaa caccccttct tatgtttgct gtcgcgcagg ctggaagccc gaggtttttt
     5041 gttaatgtgg cggcagaggt agccggacct tgtagatact gcgaaatctg gcactgaatc
     5101 ggttaactgt ccagtcgacg gccttttgtg tgatatcaat cacataaata tagggggtag
     5161 agaggtaatt gacgtgacgt tcatcacaaa acgcttgtgt aaaaaagcaa caaggcggcg
     5221 attttcgcta aaaatggcat ttaaatgtga gtagtgtcac atttttgttt tgatggtttg
     5281 ttgaatcttt gtgatctgaa tcacaagata ttttcttacg gcgttcacct gatgtgatct
     5341 acagcatgtt atgccttcgt catacaatcg ttatgtaact aagcaactca tcacctctaa
     5401 aaattattct ttggtgttat ggcgagaatc gcgaacccgg attattgtca aaatatcagc
     5461 ctgactccct tctggctgcc agcccaaaaa caacactgat aaacgtgaat ccgaggcaga
     5521 tatgtttcta aactatttcg cgttgggagt gctgatcttc gtctttctgg tgatttttta
     5581 tggaatcatc gcgatacatg acatccctta tctgattgcc aaaaagcgca accatcccca
     5641 tgccgacgct attcatacgg cgggctgggt gagcctgttt actctgcatg ttatctggcc
     5701 gtttctgtgg atctgggcga cgctctatca accggagcgt ggctggggta tgcagtcaca
     5761 tgttgcgtcg caggagaaag cgactgaccc ggaaatcgcc gcactttctg accgaatttc
     5821 ccggctggag catcaactcg ccgccgagaa aaagactgac tattccacgt tcccggagat
     5881 ctaattatgg atctattgat tgttttaact tacgtggcgc tggcgtgttt ctggtgagtg
     5941 gtttgatttt gttgatgaac tacaaccacc cttacacttt taccgcgcaa aaggcagtga
     6001 tagcgatccc catcacgcca caggtgacgg gaattgttac tgaagtcact gacaagaata
     6061 atcagcttat tcaaaagggc gaggtgcttt ttaagctcga cccggttcgt taccaggcgc
     6121 gagttgacag gcttcaggct gacctgatga cggcgacgca taatataaag actctgcgcg
     6181 cgcagctcac agaagcgcag gccaacacca cccaggtttc agcggagcgc gaccgtctgt
     6241 ttaaaaatta tcaacgttat ctgaaaggca gccaggcggc ggtgaatccg ttctcggaac
     6301 gtgacatcga cgatgcgcgg caaaatttcc tcgcgcagga tgcgctggtg aaaggctcgg
     6361 tggcggagca ggcgcagatc cagagccagc tcgacagtat ggttaacggc gagcaatcgc
     6421 agattgtgag cttaagagcg caacttactg aagcaaaata taatcttgag cagactgtca
     6481 ttcgcgcgcc gagcaatggc tacattactc aggtactgat ccgcccaggt acatacgcag
     6541 ctgccttgcc gttgcgtccg gtgatggttt tcatccccga gcaaaaacgg caaattgtcg
     6601 cccaatttcg gcaaaactcg ctgttacgtc tgaaacctgg tgatgatgca gaagtggtgt
     6661 ttaacgcgct acctgggcag gtgttccacg gcaaactgac cagtatttta cctgtcgtgc
     6721 caggcggttc ttatcaggcg cagggggtat tgcaatcatt aacggtcgtg cccggcacgg
     6781 acggtgtgct gggaaccatt gaactggacc ctaacgatga tatcgatgcc ttacccgacg
     6841 gcatctacgc ccaggtggcg gtttactccg accatttcag ccatgtttcg gtgatgcgga
     6901 aagtgctgct aagaatgacc agctggatgc attatcttta tttggatcat tgagggggag
     6961 gggggagatt atggaacttt gctccttgta aggactaatt atttttgcat attacttcat
     7021 tagctgcgat gatttttctt taatattcat tatgttaata agttatggat gagtgtgctc
     7081 tcattggatt ttttatccgt cttgttctgc cgcaggatga gactatacat cgtcgagatc
     7141 gcaatgatga tatgagttgt tcctcctgcg acggctctaa tcaaaaaaga gcaggagtgc
     7201 ttttccatct attgcgatga tggctttatt atctgaagga tgacagtcac atttaaaaaa
     7261 tatttaatct atagatcaca atgtagggtc atggaatata tgtatacaac tattttcttc
     7321 ttcactttta attatcgtat tcattataaa actaagttaa aaaaactcct ggagagtgag
     7381 ttattaatat atttttaaat aaaattaagt tatcattcat cgatgcattt tatagaatca
     7441 tcaatggcca taatagtact attaactctc aattttatat ttttatcatc actacaaata
     7501 tt
//
LOCUS       DAZGJW010000197         7421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZGJW010000197 DAZGJW010000000
VERSION     DAZGJW010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.197"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(63..886)
                     /locus_tag="ACHKIK_004540"
                     /pseudo
     CDS             complement(63..886)
                     /locus_tag="ACHKIK_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(940..2658)
                     /gene="proS"
                     /locus_tag="ACHKIK_004541"
     CDS             complement(940..2658)
                     /gene="proS"
                     /locus_tag="ACHKIK_004541"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO9085567.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2770..3477)
                     /gene="tsaA"
                     /locus_tag="ACHKIK_004542"
     CDS             complement(2770..3477)
                     /gene="tsaA"
                     /locus_tag="ACHKIK_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO9085568.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3474..3878)
                     /gene="rcsF"
                     /locus_tag="ACHKIK_004543"
     CDS             complement(3474..3878)
                     /gene="rcsF"
                     /locus_tag="ACHKIK_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO9085569.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(3996..4811)
                     /gene="metQ"
                     /locus_tag="ACHKIK_004544"
     CDS             complement(3996..4811)
                     /gene="metQ"
                     /locus_tag="ACHKIK_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO9085570.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4851..5504)
                     /locus_tag="ACHKIK_004545"
     CDS             complement(4851..5504)
                     /locus_tag="ACHKIK_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO9085571.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5497..6528)
                     /gene="metN"
                     /locus_tag="ACHKIK_004546"
     CDS             complement(5497..6528)
                     /gene="metN"
                     /locus_tag="ACHKIK_004546"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO9085572.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6716..7291
                     /gene="gmhB"
                     /locus_tag="ACHKIK_004547"
     CDS             6716..7291
                     /gene="gmhB"
                     /locus_tag="ACHKIK_004547"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO9085573.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         1814 a         1925 c         1888 g         1794 t
ORIGIN      
        1 ttttgtaggc cggataaggc gtttacgccg catccggcat tttgtgcacg aggctggctt
       61 tatcactgag tcagcccaac aaagcgaccc gccttgatat aaatcccagg cttcaggtgc
      121 ccaacatact gtaactgcgt ttctggctta aacgcagaga catcaccaat acgcatatgc
      181 atcagatcgg cagggctaat ccagcccgat tgcgccagtg taagtggatg cccggctttg
      241 gcaaaggcat ccgtgacgaa ttccgaacaa aaccacgact ttttgtctcc ttcgcccaca
      301 ctgcttaact gcgctttcgc caggccgctg acgcactttg gcgaaaatcc tcggagaacg
      361 gattcagtga gcacatctgg cgagtcacca taaagggaat aaattcgaca atgccgcgat
      421 agttataacc gctatctttg attttattgg caaacgcggt gatatctgtg gcttgttgcg
      481 gggtaagatc cgggactcgt aagacgaaaa gcttatcact atgcttcatc gcttttttaa
      541 gggaaacaat ctggacgcca gcgcctgtcg cttctgcaac gttattatca cccagaaaga
      601 ttgcaacgtg actcacagag gaagtgctga agacgcggat tccaaatgag gttaccccaa
      661 ggcttgagga gaacagcaaa tcgccgggtt tgagatcagg tgccgttatt tcttttattg
      721 attgttcggt gaaagagctt tgatgctgga atttgacagc ccatgttttt gcctcggcat
      781 ctactgctgt ggctgatggg tctggctggc tgatatcaac ggtacaagcc gaaagtaaaa
      841 gaaagctggg gagaaacagg cggcagtacg cctttggttt atccatttta tacaatccat
      901 gtaaaaaaag ggccctgaaa ttcaggaccc tttctggcat cagcctttaa tctgtttcac
      961 cagatattcg acgatgtcac cagtcttaat taactgtttc tcgccgttgc gacgatattt
     1021 atattcgata tcgtcgttgt cgaggttacg gtcgcccagc acaatagtgt gcggaatacc
     1081 gatcagttcc atatcagcaa acatcacgcc cggacgctct ttgcggtcat ccagcagcac
     1141 ttcgatacct tgtgcacgca gttcgctgta cagtttctca gcaagctctt gtacgcggaa
     1201 ggatttgtgc atgttcatcg gcagaatcgc cacctggaac ggcgcgatag cgtcaggcca
     1261 tacgatgcct cgttcgtcgt agttctgctc aatcgccgca gctaccacac gcgttacccc
     1321 gataccgtag caacccatcg tcaggatttg gttacggcca tcttcaccct gtacggaggc
     1381 tttcagtgct tcggagtact tggtacccag ctggaagatg tgaccaactt cgataccacg
     1441 tttgatcagc agcgtaccct ggccatccgg gcttggatcg ccagccacca cgttacggat
     1501 atctgcaact tccggggtag cgacatcgcg atcccagttg atgccgaagt agtgtttacc
     1561 atcgatgtta gcaccagcag cgaaatcact catcgccgca acggtacggt caatcaccac
     1621 cggaatcggc atgtttaccg gacccagtga acccggaccg gctttaacca cggcacgaat
     1681 ttcttcttcg gtcgcgaaag tcagcgggct tgcaacctgc ggcagttttt ctgctttaac
     1741 ttcgttcagc tcgtgatcac cgcgcaccag cagcgcaacc agcgggaagc tactgccttc
     1801 aaccgcttta accagcagag tcttaaccgt tttctcaatc ggcagattga actgttcaac
     1861 cagttccgcg atggttttcg cgttcggcgt atcaaccagc gtcatttcct gggtagcagc
     1921 agcgcgcggt tctttcggcg cgatagcttc tgccagttca atgttcgctg catagtcaga
     1981 ggtgtcggag aagaccacat cgtcttcacc gctctgcgcc agcacctgga attcgtgaga
     2041 ggcgctgccg ccgatagaac cggtgtcggc ttgtacggcg cggaaatcca gccccatgcg
     2101 gctgaagatt ttgctgtagg ccgcatacat tgcatcgtag gtttcctgca gggattcctg
     2161 agaagtatgg aaagagtaag catctttcat caggaattcg cgggaacgca tgacgccgaa
     2221 acgcggacgc acttcgtcgc ggaacttggt ctggatctga tagaagttca gcggcagctg
     2281 tttgtaagag ctaagctcgt tacgaatcag gtcagtgata acttcttcat gagttgggcc
     2341 gagtacgaac ggacgctcgc cacggtcaac aaaacgcagc agttccggac cgtactgttc
     2401 ccaacgacca ctctcttgcc acaaatcagc tggctgaacc accggcatcg acacctcgat
     2461 cgcaccggcg ttgttcatct cttcacgcac gatgttttcg acttttttca gaacgcgcac
     2521 gccggtcggc agccaggtat ataacccgga ggccagcttg cggatcatcc cggcgcgcag
     2581 catcagctga tggctgatca cctcggcgtc ggcaggtgtc tccttgagag tggagagcag
     2641 gtattggcta gtacgcatgt tgttacggtt ccagttggaa ggtagaacag gctcaaggcg
     2701 agcctgggac aaaaaaaagt gatttagttt accagtgcaa aagaaatgtc aaaagagaag
     2761 ggcgtgaatt taacgcggtt ccagcgcaaa gacttcaaaa cctgcgtcgg tgacgcgcca
     2821 gcgaacgtta aaatcatgta gccagacggc ataggttttg cccgtttcct cacctttacg
     2881 ataggccggg cgtgggtcct gcgccagtac ttcgcggata aacagcgtta actgcggata
     2941 acgcttctcc agcgtcaaaa gctgcttttc gacctctgcg gtaaaactca ccgccatctc
     3001 tgccgctggc gcactttgcg catagctggc actggcatcg ggaagcgatt cggcaaaggg
     3061 gagatacggt ttgatatcca ctaccggcgt accatcgacc agatccagac tgccgagctt
     3121 cagaatcacg ctgtctttat ggcaaacaac ctctttcagc tctaccagcg acataccaat
     3181 tgggttaggg cggaaagtag agcgtgttgc gaaaaccccc attctggcgt taccgccgag
     3241 gcgcggtgga cgcacagtcg gacgccagcc gccttccatc gtttgatgaa agacgaaaag
     3301 gatccataaa tggctgaacg cttccaggcc gcgaacggcg tcggcctggt tgtagggagc
     3361 aatgagatgc agttcgccgt tggcgctttt taccagacct ggctggcgcg gaacggcgaa
     3421 tttttcttta tagggcgagc gaataacgcc tatttgctcg aactggaaac tgctcatttc
     3481 gccgtaatgt taagcgcaga gccgatacat acagcctgac gatagcagcc tggcgtaccg
     3541 ctggtgactt cgcagctatg cagtaatacc gcattggcct tcattttcga ggcgttgatt
     3601 tgcatccgct tacgcgcggt tggaatgctc ggcggagagt cctgattaga ggcctggcaa
     3661 gagtcgccac tgacttcacc gagatcgcgg aacggtttgc cgactaattc ttctgcattg
     3721 gtataaattc ggaccggcgt ggcgcgcggc gctttcggtt ttgcaggctc cgctttcggc
     3781 tggggtgcag tgctttgaac gggttcgaca ggggatctgc ttaacatgga acagccgctt
     3841 agcatgagtg ctactaaaca gatcggtaaa gcacgcatag tatttcctca atgtatgatc
     3901 aaaacgtcaa tattgaatca ggagcttgta aaaatgacaa gacgggcaag cgcccgtcct
     3961 gaatgatatt acaaattgtg gaaacagcct aaaaattacc agcctttaac agcgccgccg
     4021 ttaaacactt tgtttgctgc ttcgtaaact tcatcagact gataagcctg gacgaatttc
     4081 ttcacgttct cggcgtcttt gttatcttca cgcgttacga tcaggtttac gtacggggag
     4141 tctttatctt cgacaaagat accgtctttc gctggagtca ggccaatctg gctggcatag
     4201 gtggtattga taactgccag agcgatttgc gcgtcgtcca gagatcgagg cagctgcggt
     4261 gcttccagct caacaatttt cagatttttt gggttctcaa caacatcaag aacggtcggc
     4321 agcaggccaa cgccatcttt cagtttgatc aagcccactt tttgcagcag cagcagtgaa
     4381 cgaccaagat tagttggatc gtttggcacg gcaacctgcg aaccatcctg cagttcatcc
     4441 agtgatttga ttttcttgga gtaaccagca atcggataaa caaaagtgtt gcctactgcg
     4501 accagtttgt agccacgatc tttcagttgc tgatcaaggt acggtttatg ctggaaggcg
     4561 ttggcgtcga tatcgccttt gctcaatgct tcgtttggca gaacatagtc gttgaaggtt
     4621 accagctcaa cgtccaggcc atatttgtct ttcgcaactt tctgcgcgac ttctgctacc
     4681 tgctgttcgg caccaacaat cacgccgact ttaatgtggt ttggatcttt ttcatcctga
     4741 ccgcagccta ccagtgccag tgatccaatc agggctccca ctgccgcaaa ggttttgaat
     4801 ttgaacgcca taccttattc cttcttcaat tatttatgtt gtgttgaacg ttacttgcga
     4861 gtgacagccc ggacgatgcg gtcgcctgcg aactgaatta aataaaccag aatgaccagc
     4921 aataccagta ccgtattcat caccgtcgcg ttatagccga tgtagccata ctgatagcca
     4981 atctgaccta aaccaccggc accgactgca ccacccatcg cggaataacc gaccagggta
     5041 atcagggtga tagttgccgc attcaccaga cccggcaatg cttccggtaa cagcacttta
     5101 cggacgatct gcatcggcgt ggcacccatt gcacgggaag cttcaattaa cccggttggg
     5161 atctccagca gagcgttctc gaccatacgg gcaataaacg gtgctgcacc aacggttaac
     5221 ggaacaatcg ctgcctgcaa accaatcgat gtaccgacaa taacgcgggt aaacggaatc
     5281 atccatacca gcaagataat gaacgggatg gaacggaaaa tgttcacaat cgcagaaacg
     5341 gtacgataca gcttcgcgtt agcaataatt tgccccgggc gcgtaacata aagcaggacg
     5401 ccgaccggca gaccaatcac aaagccaaaa aatccggata cgaaggtcat tgccagcgtt
     5461 tcccatacgc cacgaaccag caaccacatc atcggctcag acataaccca gtacctctac
     5521 ttttacatgg tgttcctgca gccaggcaat ggcggcttgc gtatcttgtt gtgtgccgtg
     5581 catttcagtc agcatgatgc cgaacttaac gccaccggcg taatccatct gcgcgctaat
     5641 aatgttgttg ttgacgttga aacgacgcgc ggtttcagaa agcagcgggg catcgaccga
     5701 ttgaccggta aactccaggc gcaacatcgg cacgcagtca gtaaatggct ccgcttgcag
     5761 acgttcctgg taatcttccg ggatatccag atgcagggtc gactgaataa acttctgcgc
     5821 cagcggcgtt ttcggatgcg agaacacttc acttaccgtg tcctgctcga tcagttcgcc
     5881 attgctgatg accgccacgc aatcacaaat acgcttcaca acgtccattt cgtgggtgat
     5941 caacagaatc gtcaaaccca gacggcggtt aatgtctttc agcagttcga gaatagaacg
     6001 tgtcgttgcc gggtccagcg cgctggtggc ttcatcacac agcaatactt tgggattgct
     6061 ggctaatgca cgggcaatcg ccacacgttg tttctgccca ccggaaagat tcgacgggta
     6121 gctatcatgc ttatcgccaa gaccaaccaa tgacagcaat tccgtcacgc gacgtttgat
     6181 ctcgtctttc ggtgtgttgt ccagctccag cggcagagcc acgttgccaa aaacagtacg
     6241 cgaagagagc aggttaaaat gctggaaaat cataccaatc tggcggcgag ctttggtcaa
     6301 ctcggattct gacagcgtgg tcagttcctg gccatcgacc agcacgctac cctcggttgg
     6361 gcgctccagc aggtttacac aacgtataag cgtactctta cccgcgcctg aggcaccgat
     6421 aacgccataa atttgtccag ctggcacatg caggctgacg ttgttcaacg cctggatggt
     6481 gcgggtgccc tggtggaaca ctttggtgat attcgaaagt tttatcattg attatttatt
     6541 atcgtcatta agttagtcgt ggcatctcga atgcctgaaa cgggcaacgc cgtcaatgaa
     6601 atggatgtta aggcatccag acgtctaaat caatcaggtt tatgcgaaga gcactttctt
     6661 gcaggtcgaa acatgcgata ctagcgtccc atgccttatt aaggagctat aaaaggtggc
     6721 gaagagcgta cccgcaattt ttcttgaccg tgatggcacc attaatgtcg atcacggcta
     6781 tgtccatgag atcgacaact ttgaatttat cgacggtgtt attgacgcca tgcgcgagct
     6841 aaaaaaaatg ggctttgcgc tggtggtagt aaccaaccag tctggcattg ctcgcggtaa
     6901 atttaccgaa gcacagtttg aaacgctgac cgagtggatg gactggtcgc tggcggaccg
     6961 agatgtcgat ctggatggta tctattattg cccgcatcat ccgcagggta gtgttgaaga
     7021 gtttcgccag gtctgcgatt gccgcaaacc acatccgggg atgcttttgt cagcacgcga
     7081 ttatttgcat attgatatgg ccgcttctta tatggtgggc gataaattag aagatatgca
     7141 ggcagcggtt gcggcgaacg tgggaacaaa agtgctggtg cgtacgggta aacctattac
     7201 acctgaagca gaaaacgcgg cagattgggt gttaaatagc ctggcagacc tgccgcaagc
     7261 gataaaaaag cagcaaaaac cggcacaatg attaaaagat gagcggttga aataaaaatg
     7321 catttttccg cttgtcttcc tgagccgact ccctataatg cgcctccatc gacacggcgg
     7381 atgtgaatca cttcacacaa acagccggtt cggttgaaga g
//
LOCUS       DAZGJW010000198         7322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZGJW010000198 DAZGJW010000000
VERSION     DAZGJW010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.198"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..1669
                     /locus_tag="ACHKIK_004548"
     CDS             2..1669
                     /locus_tag="ACHKIK_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017801880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO9085574.1"
                     /translation="MKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQNK
                     QELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNSFT
                     AAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNHGS
                     PQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFGDS
                     ANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVGIE
                     NWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAKYS
                     APNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFASF
                     SDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYESGA
                     HSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALKVG
                     ESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            1694..3133
                     /locus_tag="ACHKIK_004549"
     CDS             1694..3133
                     /locus_tag="ACHKIK_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO9085575.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(3190..3408)
                     /gene="marB"
                     /locus_tag="ACHKIK_004550"
     CDS             complement(3190..3408)
                     /gene="marB"
                     /locus_tag="ACHKIK_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO9085576.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(3440..3823)
                     /gene="marA"
                     /locus_tag="ACHKIK_004551"
     CDS             complement(3440..3823)
                     /gene="marA"
                     /locus_tag="ACHKIK_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO9085577.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(3843..4277)
                     /gene="marR"
                     /locus_tag="ACHKIK_004552"
     CDS             complement(3843..4277)
                     /gene="marR"
                     /locus_tag="ACHKIK_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO9085578.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            4489..5154
                     /gene="marC"
                     /locus_tag="ACHKIK_004553"
     CDS             4489..5154
                     /gene="marC"
                     /locus_tag="ACHKIK_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO9085579.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(5179..6369)
                     /gene="ydeA"
                     /locus_tag="ACHKIK_004554"
     CDS             complement(5179..6369)
                     /gene="ydeA"
                     /locus_tag="ACHKIK_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO9085580.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(6519..7312)
                     /gene="yneK"
                     /locus_tag="ACHKIK_004555"
                     /pseudo
     CDS             complement(6519..7312)
                     /gene="yneK"
                     /locus_tag="ACHKIK_004555"
                     /inference="COORDINATES: protein motif:HMM:NF033404.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
BASE COUNT         2039 a         1579 c         1775 g         1929 t
ORIGIN      
        1 tataaagacg cttaacgtca gccttttgtc tttttctatt attacagcat tgtttccatt
       61 gaacgcgatg gcaacaaaat taaccataga gcagcgcctt gaattgcttg aaaatgaatt
      121 gtcgcaaaat aaacaagagc tgaaagcaac acagaatgaa ctgggagtat ataaatcccg
      181 actttcgacc ttacaaaaaa gcatcacgga aaacaaatat aaatcagcct cgcttgctga
      241 aatatcagcg acatctcccg tcgctgataa catcaaaaat gaaaacggtg aacagaactc
      301 gtttaccgca gcgcatacta taaatggatc gcagcaagtt gccgttattg aaagtaaagg
      361 cgacaaaacc actatcgaaa gcgtgaccct gaaagatatc agtaaatata taaaggatga
      421 tattgggttc agctatcagg ggtacttccg ctcaggctgg ggtactggta atcacggctc
      481 accacaaact tatgcagcgg gttctctggg acgttttggt aatgagatga gcggttggtt
      541 tgacctgacc ttaaatcagc gtgtttataa tcaggacggt aaaacggcaa atgcggtcgt
      601 tacctatgat ggcaacgtgg gtgagcagta taacgatgcc tggtttggtg acagtgccaa
      661 tgaaaatatc atgcagttca gcgatattta cctgacaacg cgaggttttt tacccttcgc
      721 accagaggca gacttctggg tcggcaaaca taaactcccg caatatgaga tccagatgct
      781 ggactggaaa accttaacca cggatgtcgc cgcgggtgtg gggattgaaa actgggcact
      841 tggtgtaggg ctgtttgata tgtccttaag ccgtgatgat gtcgatgttt actctcgtga
      901 tttttcgcgt accagccaga tgaataccaa ttctgtggat gttcgttatc gcaatatccc
      961 gttatgggat gatgcgacat tatcattaat ggctaaatat tccgcaccta ataaaacgga
     1021 tcaacaacaa gataacgaaa atgacaacag ttattttgaa atgaaagata gctggatgct
     1081 gacttctgtt ttacggcaaa acctgcaacg cgatacgttt aatgaattta cgttacaggt
     1141 tgccaataat tcctatgcca gcagttttgc cagtttctca gatgccagta atacgatggc
     1201 gcatggtcgc tattactatg gtgatcatac caatgggatc gcctggcgtt taatctctca
     1261 gggcgagatg tatcttactg acaatattat tatggctaac gcgcttgtct attctcacgg
     1321 cgaagatgtt tatagttatg aaagtggtgc tcatagtgat tttgacagta ttcgcactgt
     1381 aataagaccg gcctggatct ggaatacatg gaatcagacg gggcttgaat taggctggtt
     1441 taagcaacag aacaaaactc agcagggtgt aacactaaat gaatcggctt ataaaacgac
     1501 actctggcat gcattgaaag tgggtgaaag cattttaggt tcacgaccag aaattcgctt
     1561 ctatggaacg tatatcaata ttctggataa cgaattatcg aattttaagt ttaatgagaa
     1621 cagcaaagac gagtttatgg ccggcatcca ggcggaagtc tggtggtaat ctattttagt
     1681 taaggagttg tgtatgtcag gatttaaaaa aggtttttta tggggcggtg cggtagccgc
     1741 gcatcagttg gaaggtggct ggaatgaagg aggaaaaggc atcagcatcg ctgatgtgat
     1801 gactgctggc gctcacgggg tgccgcgtga agtgacagaa ggcgttatcg acgggcttaa
     1861 ttatcccaat catgaagcaa ttgattttta tcatcgctat aaaacagata ttcagttatt
     1921 tgccgagatg ggattcaaat gctttcgaac ttccattgcc tggacacgaa tctttccgca
     1981 aggtgacgaa caggagccga atgaagaggg tttacaattt tatgatgatc tgttcgatga
     2041 atgccttaag cagggaatgg aacctgtggt gacgctttca cattttgaga tgccttatca
     2101 tctggtgaca aaatatggtg gctggcgaaa ccgtaaactg atcgactttt ttatccgctt
     2161 cgcatcaacg gtcttcacgc gctataaaga aaaagtaaag tactggatga cgtttaacga
     2221 aatcaataat caggtgaatt tcagcgaaag cctgtgtcca tttactaatt ccggtatctt
     2281 gtattcgcca gaggaagata tcaatgagcg cgaacaaata atgtaccagg cggtacatta
     2341 cgagttagtt gccagtgccc tggcggtaca gactggaaaa tcgatcaatc ctgaatttaa
     2401 cattggctgt atgatcgcca tgtgtcccat ctatcctctg acgtgtgcac ccaacgatat
     2461 gatgatggcc acgaaagcaa tgcatcgtcg ttactggttt actgatgttc atgctcgtgg
     2521 atattatccg caacatatgc tgaattactt tgccaggaaa ggattcaacc tcgatatcac
     2581 accagaagat aacgcgattc ttgccagtgg ttgtgtcgac tttatcggct ttagctacta
     2641 catgtctttt acgacacaat tttcgcccga taacccgcaa ctggattatg ttgaaccacg
     2701 agatttggtc agcaaccctt atatcgatac atccgaatgg ggatggcaaa ttgatccggc
     2761 agggctacgt tattcactca actggttctg ggatcatttc cagttgccgc tgtttattgt
     2821 cgaaaatgga tttggtgcgg ttgaccagag acaagctgac ggcacggtga acgatcacta
     2881 tcgcattgat tactttgctt cccatattcg ggaaatgaaa aaagccgtag ttgaagatgg
     2941 tgttgactta attggctaca ccccgtgggg ctgcattgac ctggtttctg ccggaacagg
     3001 ggaaatgaaa aagcgctacg gaatgattta tgtcgataaa gacaacgaag ggaagggaac
     3061 gctggaacgg atacgtaaag cgtcgtttta ctggtatcgg gatctcatcg ccaacaatgg
     3121 cgaaaatatt tgagacttga ggaagatatt acggagatgt caggctctgt gatcagagcc
     3181 tgacaataac tacatagcgt gttgattata atagggcacg ccgagcgcat ccgacttatc
     3241 actgccagta cccatgtgat ttaaatcaaa cggtggttgt tcctgcgatg ggggaacaac
     3301 cacgacattg gcacaagaag taacaactgg ctgcgtggtt tgttccgcaa cgccctgcgc
     3361 ggaaaagaga ataagcgcag ctgctattgc ggatgaaagt ggtttcatga ttgcctcagt
     3421 gacgttgtca cgttatcaac tagctgttgt aatgatttaa tggatgtaaa aagcgcgatt
     3481 cgccctgcat attggtcatc cggtatttat gcggcggaac atcaaagtaa tttttgaagg
     3541 ttcgggtcag agtttgttgc gactcgaagc catatcgttc tgccagatag agtatcggct
     3601 cgttactttc cttcagcttt tgcgcgattt ccgtcatctt acggctgcgg atgtattggc
     3661 ctaatgaatg accggtttct tttttaaaca tccgttgcag gtgccattta gagtaacctg
     3721 aacgctctga cactttctcc agtgacagtg gcgattccag gttgtcctcg atccagtcca
     3781 aaatgctatg aatggtaata gcgtcagtat tgcgtctgga catcgtcata cctctttttt
     3841 gtttacggca ggactttctt aagcaaatgc tcaagtgttg ccacttcgtc cgccgtcagg
     3901 ttttttgtta attcttgatg caggtcctgg ccaactaatt gatggcattg ttcgcatatt
     3961 gccgcgccgc tggtggtaag ttttaccagt acgccgcgct tatcattcgg gttcggcaac
     4021 ctttcaaccc agcctttaca gaccaggcga tccagcatac gggtcagtgc tccaaggtcg
     4081 acagacaaca cttttttcag ttcaaccgga gtaatacacg ccgcgcagcg gatagagcag
     4141 agcaccttaa actgtgccgc ggtaatatcc agcggagaca gatactcgtt aagcagacga
     4201 tctttcttct gattaaccat atggattaag cgacccaatg gaataatttc attgaacaga
     4261 tcgctggtac ttttcacatt agttgccctg gcaagtaatt agttgcaggg gataatattg
     4321 cccaggcaag tataagtcaa ataaatgaat tgaccgatgc cacgttttgc taaatcggtt
     4381 caagttttgg tcaatgcgat gcaaggttag ctaaagagtg acactttatc cttgtttacc
     4441 ccttatactt ttcgctgata acccagatac acaggataac aaccaccaat gttagatttg
     4501 tttaaagcaa ttggcctggg gctggtggtg ttactgccgt tagctaaccc attaacaact
     4561 gtcgcgttat ttcttggcct ggcaggcaac atgaacagtg ccgaacgtaa tcgtcagtcg
     4621 ttgatggcct cggtgtacgt atttgccatc atgatggtgg cgtattacgc cgggcaactg
     4681 gtgatggata catttggcat ttcgattccc ggtctgcgaa ttgcaggcgg cttaatcgtc
     4741 gcgtttatcg gttttcggat gctctttccg caacagaaag cgattgattc accggaggcg
     4801 aaaagcaagt cggaagagct ggaagatgaa cccagtgcca atattgcctt tgtgccgcta
     4861 gcaatgccaa gtaccgctgg tccgggaacc atagcgatga ttattagctc ggcgtccaca
     4921 gtgcgtcaga gttcaacctt cgctgattgg gtactgatgg ttgcgccgcc gctgatcttt
     4981 ttcctggtgg cggttatttt gtggggaagc ctacgcagct ccggcgcaat tatgcggctg
     5041 gtgggcaagg gtggcattga agcgatctcc cgtctgatgg gattcctgct ggtatgtatg
     5101 ggtgtgcagt ttattattaa cggcatcctg gaaatcatta aaacgtacca ttaaaaaagg
     5161 cccgaatggg cctttcaact attgcgtctg ttcttcgagt gtcactggcc agcggcgaaa
     5221 tataatgatt gaccaaatta acgcggcaaa agcaggcacc gcgcccacat aaccaatcat
     5281 cgacattgac cagtgcaaac tcacctgatt acctaccaac gcacccgccc cgattccaat
     5341 attaaatatc ccggagaata gcgccatcgc gacgtcggta gcatctggtg ccagcgccag
     5401 cactttaacc tgcataccaa gcccgatgat catcatcgcg atcccccaga aaatactcag
     5461 caccccgagg tgtatttcac tgttcgccgc aggtaacagc aatgccaggc acaccagcaa
     5521 cagcgcaatc gccgtactca ccaacgcaga cgcatactga ttacccagtt taccgaaaat
     5581 cacgctgcca ataatgcccg caccaccgag taataacagt aatgccgtgg caaagttggc
     5641 gctgaatccc gcaatgtttt gtacaaaagg ctcgatatag ctgtatgccg tgtaatgggc
     5701 ggtgacaacc accacagtta acaaataaat gctcatcaat gccgggcggc ggaacaatag
     5761 cgggaggctt ttcagtgaac cggaatgctc actgggcagt aagggaagta acttaatcag
     5821 gcacaaaagg gtgataagcg ccccaatacc aatcgcgaag aaggtcattc gccaaccgaa
     5881 atactggccc acaatgcgcc cgagaggtaa acctaagacc atcgccagtg ctgtaccggt
     5941 ggcaattaaa ctcaatgcct gtgctcgctt cccggccgga gccatacgga tcgccagaga
     6001 cgccgtaatc gaccagaaaa tcgcatgtgc aaaagccaca ccaatgcgac tgatcaccag
     6061 aacggtaaag ctccacgata aaaacgacag tacgtggctg gcaataaaca ccacaaacag
     6121 gcagatcagt aatttgcgcc gttcaacctg gctggtcatt aacataaaag gcaatgacat
     6181 tagcgccact acccatgcat aaatggtcaa cataatgccg acctgagcgg tttgcatgtg
     6241 aaaactttgc gcaatgtcag agagcaggcc aacagggaca aattcggtgg tgttgaagat
     6301 gaaggcggcg actgccagcg taacgacccg tagccacgcc actttgcggg aaacagtgtt
     6361 tgttgtcata acagattatc gggtgagttg cgaaaaagtg atgaaatgat cttaaaacgg
     6421 ttaatgatgt gaatacaatg catttgtgat ctggatcgcg ttttccgttt ctctgacgct
     6481 gattctgttc cgcgtcagag agtcggtaac atcacggttt aatgacgttc agggaggtct
     6541 ggcgttttgg cggttgcggg tataggtggt ctgacagggg agcgaggcct gtttttcacc
     6601 aggtcttcgt gagtaacttt tggcggcgtt tcatttttta caaacagttt gagggtcctg
     6661 cgcgccatat gtttgataat tttatcatca cttaatgatg ctggcatcaa cacgtcattc
     6721 agatcacttt cattgggatt cataaattgg tgatagtgca ataacatatt ggcgaaaaat
     6781 aaaaaaaagt cctgaaaatg aggagattct tgttctgcta aaagggataa aaagcagatg
     6841 cagacttcgg gtagtacatg tttgttagaa cctaatgaat ccatcaggct ttgcaataaa
     6901 tcctctttta attcgccgtc tgttatgtcg agaatggcgg tgatcaagtt aggatcttta
     6961 ttgacgaacc atctgtgaac ctcagagtta ctccataatc gggggagtat gacaaaatta
     7021 ccgcgagaga taaaattcag gacaagcaaa tcaaactggc gtaagtaatc tgcagcggta
     7081 tggcgatccg gaggcagggg ttcaatatgt ggtgtatgcg ctcttttgat gggaacgcca
     7141 aggttgatca ttggaaattg ttcaataaaa tcattcagaa acagaaatag cacacgctcg
     7201 acgccggtct gaaatacgcg ccccatagtg acaggctttg ggttgggagc gcaaaaatat
     7261 taatttctcc atcatggcga aaattatcta aataaatagg gcttaaattt attactacct
     7321 ga
//
LOCUS       DAZGJW010000199         7302 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZGJW010000199 DAZGJW010000000
VERSION     DAZGJW010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.199"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            230..832
                     /locus_tag="ACHKIK_004556"
     CDS             230..832
                     /locus_tag="ACHKIK_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFO9085581.1"
                     /translation="MKNDKAWIGDLLGGPLMSRESRVIAELLLTDPDEQTWQEQIVGH
                     NILQASSPNTAKRYAATIRLRLNTLDKSAWTLIAEGSERERQQLLFVALMLHSPVVKD
                     FLAEVVNDLRRQFKEKLPGNSWNEFVNSQVRLHPVLASYSDSSIAKMGNNLVKALAEA
                     GYVDTPRRRNLQAVYLLPETQAVLQRLGQQDLISILEGKR"
     gene            829..1431
                     /locus_tag="ACHKIK_004557"
     CDS             829..1431
                     /locus_tag="ACHKIK_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFO9085582.1"
                     /translation="MIDPVLEYRLSQIQSRINEDRFLKNNGSGNEIGFWIFDYPAQCE
                     LQVREHLKYLLRHLEKDHKFACLNVFQIIIDMLNERGLFERVCQQEVKVGTETLKKQL
                     AGPLNQKKIADFIAKKVDLAAQDFVILTGMGNAWPLVRGHELMSALQDVMGFTPLLMF
                     YPGTYSGYNLSPLTDTGSQNYYRAFRLVPDTGPAATLNPQ"
     gene            1443..5084
                     /gene="brxC"
                     /locus_tag="ACHKIK_004558"
     CDS             1443..5084
                     /gene="brxC"
                     /locus_tag="ACHKIK_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFO9085583.1"
                     /translation="MNIEQIFEKPLKRNINGVVKAEQTDDASAYIELDEYVITRELEN
                     HLRHFFESYVPATGPERIRMENKIGVWVSGFFGSGKSHFIKILSYLLSNRKVTHNGTE
                     RNAYSFFEDKIKDALFLADINKAVHYPTEVILFNIDSRANVDDKEDAILKVFLKVFNE
                     RIGYCADFPHIAHLERELDKRGQYETFKAAFADINGSRWEDERDAYYFISDDMAQALS
                     QATQQSLESSRQWVEQLDKNFPLDINNFCQWVKEWLDDNGKNILFMVDEVGQFIGKNT
                     QMMLKLQTITENLGVICGGRAWVIVTSQADINAAIGGMSSRDGQDFSKIQGRFSTRLQ
                     LSSSNTSEVIQKRLLVKTDEAKAALAKVWQEKADILRNQLAFDPTTTTALRPFTSEEE
                     FVDNYPFVPWHYQILQKVFESIRTKGAAGKQLAMGERSQLEAFQTAAQQISAQGLDSL
                     VPFWRFYAAIESFLEPAVSRTITQACQNGILDEFDGNLLKTLFLIRYVETLKSTLDNL
                     VTLSIDRIDADKVELRRRVEKSLNTLERLMLIARVEDKYVFLTNEEKEIENEIRNVDV
                     DFSAINKKLASIIFDDILKSRKYRYPANKQDFDISRFLNGHPLDGAVLNDLVVKILTP
                     KDPTYSFYNSDATCRPYTSEGDGCILIRLPEEGRTWSDIDLVVQTEKFLKDNAGQRPE
                     QATLLSEKARENSNREKLLRVQLESLLAEADVWAIGERLPKKSSTPSNIVDEACRYVI
                     ENTFGKLKMLRPFNGDISREIHALLTVENDTELDLGNLEESNPDAMREVETWINMNIE
                     YNKPVYLRDILNHFARRPYGWPEDEVKLLVARLACKGKFSFSQQNNNVERKQAWELFN
                     NSRRHSELRLHKVRRHDEAQVRKAAQTMADIAQQPFNEREEPALVEHIRQVFEEWKQE
                     LNVFRAKAEGGNNPGKNEIESGLRLLNAILNEKEDFALIEKVSSLKDELLDFSEDRED
                     LVDFYRKQFATWQKLGAALNGSFKSNRSALEKDAAAVKALGELESIWQMPEPYKHLNR
                     ITPLIEQVQNVNHQLVEQHRQHALERIDARIEESRQRLLEAHATSELQNSVLLPMQKA
                     RKRAEVSQSIPEILAEQQETKALQMDADKKINLWIDELCKKQEAQLRAANEAKRAADS
                     EQTYVVVEKTVIQPVPKKTHLVNVASEMRNAAGGEVLETTEQVEKALDTLRTTLLAAI
                     KAGDRIRLQ"
     gene            5130..>7302
                     /gene="pglX"
                     /locus_tag="ACHKIK_004559"
     CDS             5130..>7302
                     /gene="pglX"
                     /locus_tag="ACHKIK_004559"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFO9085584.1"
                     /translation="MNTNNIKKYAPQARNDFRDAVIQKLTTLGIAADKKGNLQIAEAE
                     TIGETVRYGQFDYPLSTLPRRERLVKRAREQGFEVLVEHCAYTWFNRLCAIRYMELHG
                     YLEHGFRMLSHPETPTAFEVLDHVPEVAEALLPENKAQLVEMKLSGNQDEALYRELLL
                     GQCHALHHVMPFLFEAVDDEAELLLPDNLTRTDSILRGLVDNIPEEDWEQVEVIGWLY
                     QFYISEKKDAVIGKVVKSEDIPAATQLFTPNWIVQYLVQNSVGRQWLQTYPDSPLKDK
                     MEYYIEPAEQTPEVQAQLAAITPASIEPESIKVLDPACGSGHILIEAYNVLKNIYEER
                     GYRGRDIPQLILENNIFGLDIDDRAAQLSGFALLMMARQDDRRIFTRDVRLNIVSLQE
                     SLHLDIAKLWQQLNFHQQVQTGSMGDMFAENTALTQTNSAEYQLLMRTLKRFVNAKTL
                     GSLIQVPQEEEAELKVFLDALYRLEQEGDFQQKSAAKAFIPFIQQAWILAQRYDAVVA
                     NPPYMGSKYQSLSVKKHLKDNFTGYDKDLFSAFIVRNLELSKQGAQLGFMTPFVWMFI
                     TTYEELRKKLIEQEIITSLVQLEYSGFDGATVPICTFTLQKGHINHFTGSYIRLSDFR
                     GASNQGPKTLEAIQHHNCGWFYEAQPDNFKKIPGTPIAYWLGQNINIFDNDKIANSWF
                     SGGRIKTHNGDKYIRYIWETSRLSSKWKKLIKGGDYKKHFGN"
BASE COUNT         2053 a         1712 c         1797 g         1740 t
ORIGIN      
        1 gtaatcgatt aagttgatgc atttaatggg aatgatatag ggtcatttcc agtctcactt
       61 atagaaatgg ctaaagcatg actctcgcca aaaccgttta tgtgttgtac ataacgcgat
      121 catccctctc acaaattgcc ttttctcatg gcatctcgcc cggtccccca ttacaatcac
      181 tttttgtttt ttgcgagctg cattccagtc ttcagagggt ttttcgatga ttaaaaatga
      241 caaggcatgg ataggagact tgctgggcgg accgctcatg agcagggaaa gccgcgtcat
      301 tgccgaactg ttgctaaccg atcccgatga acagacatgg caagagcaaa ttgttggcca
      361 caacatttta caagcctctt ctcctaacac cgcaaaacgt tacgcggcaa caatcaggct
      421 tcgcctgaac acgctggata aaagcgcgtg gacattgatt gccgaaggta gtgaacggga
      481 acgccaacaa cttctgtttg tggctctgat gctacattcg ccggtagtta aggattttct
      541 ggctgaagtg gtgaacgatc tgcgcaggca gttcaaggaa aagttgcctg gcaatagctg
      601 gaacgaattt gtgaatagcc aggttcgcct acatccggta ctcgccagct actcagattc
      661 atctattgca aaaatgggaa acaatctggt gaaggcgctt gctgaagcgg gttatgtgga
      721 tacgccccgc agacgtaacc tgcaggcagt ttacctttta ccggaaactc aggcagtgtt
      781 acagcgcctg ggacaacagg acttgatatc tattctggag ggaaaacggt gatagatccc
      841 gttcttgaat atcgcctgtc tcaaatccag agtcgcatta acgaagatcg cttcctcaaa
      901 aataacggct ccggaaatga aattggtttt tggatctttg attatcccgc gcagtgcgaa
      961 ctgcaggtac gggagcattt gaaatatctg ctccggcatc tggaaaagga ccataaattt
     1021 gcctgtctga atgtcttcca aatcatcatc gatatgctca atgaacgcgg ccttttcgag
     1081 cgcgtctgcc agcaggaagt caaagtgggt actgagacgc tgaaaaagca gcttgctggt
     1141 ccgttaaatc agaaaaagat cgctgatttt atagcgaaaa aagtcgatct ggctgcccag
     1201 gattttgtca ttcttaccgg catgggcaac gcctggccat tagtacgcgg tcatgaactg
     1261 atgagtgcct tgcaggatgt catggggttc accccactgc tgatgtttta tcctggcacc
     1321 tacagcgggt acaacctttc cccgctcaca gacaccggtt cacaaaatta ttatcgcgct
     1381 ttcagactgg taccagatac gggacccgca gcaacattga atcctcaatg aagagcataa
     1441 caatgaatat tgaacagatt tttgaaaaac ctctaaaacg aaatataaac ggggtagtca
     1501 aagcagagca aaccgatgat gccagcgcgt acatcgagtt agatgaatat gtcatcaccc
     1561 gcgaactgga aaaccatctt cgccatttct tcgaatccta tgttcctgcc actggcccgg
     1621 aacggatccg tatggaaaac aagatcggcg tatgggtttc aggcttcttc ggttcaggta
     1681 aatcgcactt tattaagatt ctttcttatc ttttatctaa ccgcaaagtt acacataacg
     1741 gtacggaacg taatgcttac tccttctttg aagataaaat caaagatgca ttattccttg
     1801 ccgatattaa caaagcggtg cattacccga ctgaagtcat tctgttcaat attgattcgc
     1861 gtgccaacgt agatgacaaa gaagatgcca ttcttaaagt cttcctgaaa gttttcaacg
     1921 aacgcattgg atactgcgct gattttccgc atattgccca tcttgagcgc gagctggata
     1981 aacgcggtca gtatgaaacc tttaaagccg cgtttgccga tatcaatggc tcgcgctggg
     2041 aagacgagcg cgacgcttac tacttcatca gcgatgacat ggcacaagca ttaagccagg
     2101 ccacgcagca gagtcttgaa tcctcccgcc aatgggtgga acaactcgac aaaaacttcc
     2161 cgctggatat caataatttt tgccagtggg taaaagagtg gctggatgac aatggtaaga
     2221 acatcctctt tatggtggat gaagtcggtc agttcattgg caaaaatacg caaatgatgc
     2281 tgaagctgca gactattact gaaaaccttg gggtaatttg cggtggccgc gcatgggtta
     2341 tcgtgacttc gcaggccgat atcaacgcgg caatcggtgg tatgagcagt cgcgacggac
     2401 aggacttctc caagatccag gggcgcttct ctacacgcct gcaactttcc agctctaaca
     2461 catcagaagt tatccagaaa cgtttgttgg taaagactga cgaagcaaaa gcggcactgg
     2521 caaaagtgtg gcaagagaaa gccgatatcc tgcgtaacca gctggctttt gaccctacaa
     2581 caactactgc actacgtcct tttaccagcg aagaagagtt cgttgacaac tacccgtttg
     2641 tcccgtggca ctatcagatt ctgcaaaaag tgtttgaatc tattcgaacg aaaggtgcag
     2701 cgggtaaaca attggccatg ggtgagcgtt ctcagctgga ggcattccag acggcggcgc
     2761 agcaaatctc agcgcaaggg ctggattctc tggtgccttt ctggcgcttc tatgccgcca
     2821 ttgagagctt cctggaacct gccgttagcc gcaccatcac tcaggcttgc cagaatggca
     2881 ttcttgatga attcgatggc aacctgctta aaacgctgtt cctgatccgc tatgtggaaa
     2941 cgctgaaaag caccctggat aacctggtca cattgtctat cgataggatc gatgccgata
     3001 aagttgagtt gcgccgccgg gtcgaaaaaa gtctgaacac gcttgaacgc ctgatgctca
     3061 ttgcgcgcgt tgaagataaa tatgtgttcc tgaccaacga agaaaaagag atcgaaaacg
     3121 agatccgtaa cgttgatgtc gatttctctg cgatcaacaa aaaactggca tcgatcatct
     3181 ttgatgacat tctgaaaagc cgtaaatatc gttatccggc taacaagcaa gactttgata
     3241 tcagtcgctt cctgaacgga cacccattag acggcgcagt gcttaacgat ctggtagtga
     3301 agatcctgac ccctaaagat ccgacctatt cgttctataa cagcgatgcg acctgtcgcc
     3361 cttatacgtc agaaggcgac ggctgtattt tgattcgtct gcccgaagag ggccgtacct
     3421 ggagcgatat tgatttagtc gtccagactg aaaagttcct caaagataac gccgggcaac
     3481 gtccggaaca ggcaaccctg ctctcagaaa aagcgcgtga aaacagcaac cgggaaaaat
     3541 tactccgtgt tcagttggaa tcactacttg cagaagcaga tgtctgggcg attggcgagc
     3601 gcttaccgaa aaaatcttcc acaccatcga acattgtcga tgaagcctgc cgttacgtga
     3661 ttgaaaacac cttcggcaag ctgaagatgc tgcggccttt taacggcgac atctcccgtg
     3721 aaattcatgc attactgacg gttgagaacg acaccgaact ggatctcggt aacctcgaag
     3781 agtccaaccc cgacgccatg cgcgaggtag aaacctggat caacatgaat atcgaataca
     3841 ataaacctgt gtatttacgc gatattctga accatttcgc gcgtcgccct tatggctggc
     3901 ccgaagacga agtgaaactg ctggtagccc gtctggcctg caaaggtaag ttcagcttta
     3961 gccagcaaaa caacaacgtc gagcgaaaac aggcgtggga gttatttaat aacagccgcc
     4021 gccatagcga attgcgtctg cataaagttc gccgtcatga tgaagcgcag gtgcgtaaag
     4081 ccgcgcaaac catggctgac atcgctcagc agccgtttaa cgaacgggaa gagccggcgc
     4141 tggttgaaca tattcgtcag gtatttgaag agtggaagca agagctgaac gtattccgcg
     4201 ccaaggcaga gggcggaaac aatccgggga aaaacgagat tgaatccggt ctgcgcctgc
     4261 ttaatgccat tcttaatgag aaagaagatt ttgccctgat cgaaaaagtc tcatcgctga
     4321 aagatgaact tctggatttc agtgaagacc gtgaagattt ggtcgacttc taccgtaagc
     4381 aattcgccac ctggcaaaaa ctgggtgctg cgctgaatgg cagctttaaa tctaaccgca
     4441 gcgcgctgga aaaagacgcc gcagcggtta aagcgctggg tgagctggaa agcatctggc
     4501 aaatgccgga accttataag catctcaatc gcatcacgcc gttgattgaa caggtccaga
     4561 acgtcaacca tcagttagtc gaacagcacc gccagcacgc cctcgaacgc attgacgccc
     4621 gcattgagga aagccgtcaa cgcttgctgg aagcgcacgc cacgtcagaa ctgcaaaaca
     4681 gcgttctgct gccgatgcaa aaagccagaa aacgcgctga ggtcagccag tcgattccgg
     4741 aaattttggc ggaacagcaa gagacaaaag cgctgcaaat ggatgcagat aaaaagatta
     4801 atctgtggat cgatgagctg tgtaaaaagc aagaagcaca actccgggca gcaaatgaag
     4861 ctaaacgcgc tgccgactca gaacagactt atgttgtggt ggaaaaaacc gttatccaac
     4921 cggtaccgaa aaaaacgcat ctggtgaatg tcgccagtga gatgcgtaac gccgccggtg
     4981 gtgaggttct ggaaaccacc gaacaggtgg aaaaggcgct cgacacgtta cgcacaacgc
     5041 tgctggccgc cattaaagca ggcgatcgaa ttcgccttca gtaactccca tttcagggca
     5101 gcactctgct gccctttgca ggattttcta tgaatactaa taacattaaa aaatatgccc
     5161 cacaggcccg taacgatttc cgcgatgcgg tgattcagaa gctaacgacg cttgggatcg
     5221 ctgcagataa aaaaggcaat ttgcagattg ccgaggccga aaccattggc gagaccgtgc
     5281 gttacggtca gtttgattac ccgttatcga cccttccccg ccgcgaacgg ctggtaaaac
     5341 gcgcccgtga gcagggtttt gaggtgctgg ttgagcactg cgcctacacc tggtttaacc
     5401 gcttatgtgc aattcgctat atggagctac acggttatct tgagcacggc ttccgtatgt
     5461 tgtcccaccc ggagacgccg accgcgtttg aggtgctgga tcatgtgccg gaagtggcag
     5521 aagccctgct gccggaaaat aaggcgcagc tggttgaaat gaagctttcc ggtaatcagg
     5581 acgaagccct gtaccgcgaa ctgctgctgg ggcagtgcca cgccctgcac cacgtgatgc
     5641 cgttcctgtt tgaagcggta gatgacgaag cggaactgct gttgccggat aacctgaccc
     5701 gtaccgactc tattctgcgt gggctggttg ataatattcc tgaagaagac tgggagcagg
     5761 tagaggttat cggctggctg tatcagttct atatttcgga aaagaaagat gctgtgattg
     5821 gcaaagtggt gaagagcgaa gatattcctg ccgccaccca gctgtttacg ccaaactgga
     5881 ttgtgcagta tctggtacag aactccgttg gccgccagtg gttgcagacc tacccggact
     5941 cgccgctgaa agacaaaatg gagtactaca ttgagcctgc ggagcaaacg ccggaagtgc
     6001 aggcacagct ggcggcgatt accccagcca gcattgaacc cgaaagtatt aaagtgctcg
     6061 acccggcctg cggttccggt catattttga ttgaagccta taatgtgctg aaaaatatct
     6121 acgaagagcg tggttatcgc gggcgtgata ttccacaact gattctggaa aataatattt
     6181 ttggtcttga tatcgacgac cgcgcagccc aactttccgg ttttgcatta ttaatgatgg
     6241 cgcgtcagga tgaccgcaga atatttaccc gcgatgtacg tctgaatatt gtctctttgc
     6301 aggaaagcct gcatctggat atcgccaaac tctggcagca actgaatttc caccagcagg
     6361 tacaaaccgg cagtatgggg gatatgtttg ctgaaaatac cgcgttaacc caaactaaca
     6421 gcgcagaata tcagctgctg atgcgcacgt tgaaacgctt tgtgaatgca aaaacgctgg
     6481 gatcgctgat tcaggtgccg caggaagaag aagcggaact gaaagtattt ctggacgcgc
     6541 tgtatcgcct ggaacaggaa ggcgatttcc agcagaagtc ggcggcgaaa gcgtttattc
     6601 cgtttattca gcaggcgtgg attctggcac agcgatatga tgcggtggtg gcgaatccgc
     6661 cgtatatggg aagcaaatac caatcattaa gtgtaaaaaa acatcttaaa gataatttta
     6721 ctggttatga taaggattta ttttcagcct ttatagtcag aaacttagaa ctttcaaaac
     6781 aaggggctca acttggattc atgacgccat ttgtctggat gtttatcact acgtacgaag
     6841 aattaagaaa aaaactgata gagcaagaaa ttattacatc gcttgtgcag cttgaatatt
     6901 caggctttga cggtgcaact gtgccaattt gcacattcac attacagaaa ggtcatatta
     6961 accattttac tggtagctat atccgcctct ctgattttcg tggtgcatca aaccaaggtc
     7021 ccaaaactct tgaagcgata caacaccata attgtggatg gttttatgaa gcgcagccag
     7081 acaattttaa aaaaatacca ggaacaccaa tagcctattg gctaggccag aatataaata
     7141 ttttcgataa tgacaaaata gctaattcat ggttctctgg tggtagaatt aaaactcaca
     7201 atggtgataa atatataaga tatatatggg aaactagtcg tctttcgagt aaatggaaaa
     7261 aactaatcaa aggtggagat tacaaaaaac attttggcaa tg
//
LOCUS       DAZGJW010000200         7275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZGJW010000200 DAZGJW010000000
VERSION     DAZGJW010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.200"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            189..1124
                     /locus_tag="ACHKIK_004560"
     CDS             189..1124
                     /locus_tag="ACHKIK_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO9085585.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELVAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLARSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(1180..1722)
                     /locus_tag="ACHKIK_004561"
     CDS             complement(1180..1722)
                     /locus_tag="ACHKIK_004561"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9085586.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1706..2017)
                     /locus_tag="ACHKIK_004562"
     CDS             complement(1706..2017)
                     /locus_tag="ACHKIK_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO9085587.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(2202..3092)
                     /gene="cyoE"
                     /locus_tag="ACHKIK_004563"
     CDS             complement(2202..3092)
                     /gene="cyoE"
                     /locus_tag="ACHKIK_004563"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO9085588.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(3104..3433)
                     /locus_tag="ACHKIK_004564"
     CDS             complement(3104..3433)
                     /locus_tag="ACHKIK_004564"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO9085589.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(3433..4047)
                     /locus_tag="ACHKIK_004565"
     CDS             complement(3433..4047)
                     /locus_tag="ACHKIK_004565"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO9085590.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(4037..6028)
                     /gene="cyoB"
                     /locus_tag="ACHKIK_004566"
     CDS             complement(4037..6028)
                     /gene="cyoB"
                     /locus_tag="ACHKIK_004566"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO9085591.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(6050..6997)
                     /gene="cyoA"
                     /locus_tag="ACHKIK_004567"
     CDS             complement(6050..6997)
                     /gene="cyoA"
                     /locus_tag="ACHKIK_004567"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO9085592.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         1992 a         1784 c         1796 g         1703 t
ORIGIN      
        1 attttttatc ttacgaatat gctagtttta tctcactaaa taaacgattt ggtgacatgg
       61 atgataatgt ttcaggctaa atgtgtgctt attacttgga cctttatggc actgtattta
      121 ttcattaaat ggatatttgg tggttaatta ttatggaaat atatgacagt gaggacataa
      181 aggtatacat gttaactaaa agaatattat tttctgtaat gctgatagtt tcaccaagcg
      241 tggtggccag cgagaaagcg catgaattgt atgacagcat atacggtggt aaacctgctc
      301 ccgatgttat aaatacactg cataaaatgg ctgaatcggg agatatcgac gcgcagagcc
      361 tgttaggctg ggaatattat cagcctcgtt atgataccaa acctgatgtt caggaagcga
      421 ttaaatggtt cgagttagtg gctaaacaag gtgacagaga agctccgtta gcgctggggg
      481 gtatctacta cgacggtgag caggtgcggg tggattatgc caaagcgtat gcactattta
      541 atcaggccgc gcagcacggt gtgaatttag caaggtccag gttgggcatt atgtacgcca
      601 atggtcagta tgttgaggta gattgtaaga aagcgaagga atatcttgat aaaggtgtcc
      661 acatttatgg tggcccagaa gactttctgg ctacttgtcg aaaagacatg attgacagaa
      721 aaacggttga cgatacgtta cccgtgatta cggttacccg ttctgggatg cgagataatt
      781 ttttagataa aggtttttcc tgcatggata gcctcttcgc caccaccaat aaattaggcg
      841 aagtggccaa tcttcgcgtg acattcagca ttcgtcgccc atccggtaag gagattaacc
      901 aaacggttgg cttcgcgcct ttcggtttaa accggctgaa tattagcttt actgattacc
      961 tatttggttc atttaccagt aattcgtctc ttattcttta taaaccggaa tttgagcgaa
     1021 aatcttgtgc aactgtaagg accacaatcg ttgccgcaac ggcaaccatt aacggcaagg
     1081 atgtggagtt gctgaaagcg ggggcaattg aacaaaagtg gtaacgctgc tcagcccccg
     1141 gctgaggcaa aacttgccct ggcgggtggc gataataatc taagccgcga gaatatgctg
     1201 aatcgccaaa aacataggta cagcaccaaa agcatttggt gtcgctgaaa gctgaacaaa
     1261 gccgagacga gcataaaaat taatggcggc agggtcagca tcaagataaa cccctttaat
     1321 tggtaatgcc tggtgaatta tttttacatg ttcaaaaaaa tcacatagta ggtcctgacc
     1381 aaagccccgc ttttgatact tccgtgctac ccccaacatg actaatctga caacaccaat
     1441 ttctgaaggt tgtgaaccct gaaggacgcc agaaacgcgt tgtttttcca gcgaatatgc
     1501 cgtaaaagta cagatgccga tcagttcacc actttgtctg tcaataagtg ctttagccgc
     1561 acagtcgcta ttacgcacac ttttctttag cgatgcgcgt acaaatttat cgataacagg
     1621 gttaccgcaa tcaaactgtt tttgcccagg ataagtaata tcagactgat aattacagga
     1681 gaggactatg ggcagagtaa tctcttcatg cttgtcgacc atcgctgttt ttccttttca
     1741 ttaacgcctt gagggcgagc gtcggttgag caggttcagc gatgagttgg ttcatcagtt
     1801 cccagctttg attcgaaagc tcacggcgac gttggttatc aagcacactt tcggcgcgtt
     1861 ccatagcagc gctcaaaata aaagcgctta aatctacccc aacggcttgc gcagcttcac
     1921 gaagtttgtt tttggcgtcg ggactagttt tgagttcgac ccgcgcattt ttcagcgttt
     1981 tatcgacatc gggtagagtg gctgtttttc caggcattat gacctctttg attaactgaa
     2041 tgaaatcgta gggagtcgat ctcatacata atacggctat caaccgtatg attaatttat
     2101 gttcatctgc tcactctgtc aagacatacg gttattagcc gtatctctga tctcagacaa
     2161 taaaaaaggt gccgtagcac ctttttaata gagaggtttt gttaccacac agcagccagc
     2221 agcgtatgcg agtccggtac cataaaatca acggacatca tcaccgagag ggcagtgatg
     2281 gcgatgatag agaagccgaa cagcttgcgc gcccagattc tgtcatcagc aactttataa
     2341 ccgcgcagag ccatacctaa ccaccagacg ctaaccgccg cggcgaccac cagatattta
     2401 tatccagcgt aaccgccaag agagagcatc agcgtggcaa cggcaaaggc gatgatatac
     2461 agcgtgatgt gattcttcgc caccgaaatg ccttttacca ctggcaatac cggaatgttt
     2521 gccgcctggt aatccttaaa gcggaaaatg gcgatggcat aggagtgagg catctgccac
     2581 aggctgaaga tagccagcag gatcgctgcg ccgctatcga actcgccggt tactgcacag
     2641 tagccaatca ccggcggcgc agcgccggag agcgaaccaa tcaatgtgcc gtagactgag
     2701 tggcgtttca tgtacaggct atagacgccg acataaacca caaagcccat cacccccagc
     2761 cagcaggcca gcggattcgc gccaaaccac agcagcataa agccagcaat acccagcaag
     2821 gtggcgtaca ccagcgagac agcaggagag atcaggcctt taaccagcac ccgattcttc
     2881 gtcctttcca tctttctgtc gatatccctg tcgatgtagt tgttaaacac acaacccgac
     2941 gccacaacca gtgacacccc aaccagcgtg tagataaaca ggggataatc aatgctgccc
     3001 tttgaggcca gcaggaatcc cccaatcacc gagatcaggt tgccaaagat gatgcctggt
     3061 ttcgttactt gcaggtattg cttaaacatc ataaccgccg ctcttagtgc atcatcatgt
     3121 tgtagttgag gttccacata atccagatgg agcctacaac caggatagcg atgattagca
     3181 cggtgaagac aaacgccgtc atgttccagc cttcatctga tttggtattc atgtgcagga
     3241 agcacaccag atgcaccaga acctgtacca ctgccattgc caggattgtt cccagaatta
     3301 cggccggaga ggcagcccct gtcatcacca tccagaacgg aatcaccgtc agaatgatcg
     3361 acaggataaa gcctgtcatg taggttttta cgctgccatg ggacgcgccg ctgtgatcgg
     3421 tagaatgact cattacatcg cccccatcag ataaacaaca gtgaacacac agatccaaac
     3481 cacatccagg aagtgccaga acaggctcag gcacatgatg cgggtacggt tagtgctggt
     3541 caggccgcga cgagcgattt gtaccatcag caccgccatc cagataagac cggaagtgac
     3601 gtgcagaccg tgcgtgccga ccagcgcaaa gaacgctgac aggaagccgc tgcgatccgg
     3661 acccatgccg ttaacaatca ggtgatggaa ttcgtagatt tccatcccga taaatccggc
     3721 accaaacaac caggtcaacg ccagccagga gataacctgg cttttgttgt ttttgtacat
     3781 ggcgatagcc gccatgccgt aggtgatgga gctgaacaac agcaagaaag tttcaaccag
     3841 aacgaacggc agttcgaaaa tgtccttacc tgtcgggccg cctgcggtgc cgttcaccag
     3901 aacggcatag gtagcaaaca agattgagaa cagaatgcag tcgctcatca ggtagatcca
     3961 aaatccgaag attttggttc cgcctgcatc gtggtgcccg tgttcgtgcg cgtgggcagt
     4021 cgcgtgcgtc aaagtatcag ttgccatttt tcagccctgc cttagtaatc tcatcgaaat
     4081 gctggttttc cagtttttcg atttctgcca ccggcacgta gtaatccacg tcctcgtcga
     4141 agcttttcac gatccaggtg atgatcatgc ctgcgaagcc aacaatcgcc agccaccaga
     4201 tatgccagat catggcgaaa ccgaagatgg tggagaaagc tgcaatgacg atacctgcac
     4261 cgctgttttt cggcatatga atttcttcat agtggtcagg ctttttatac gcttcgcctt
     4321 tctctttcat ttcccagaat gcatcacgct cgtgaacgtg cggcacaacg gcaaagttat
     4381 agaacggagg cggggaagag gttgcccact ccagcgtacg gccaccccac gggtcgccag
     4441 tcaggtcacg gttctggtcg cggtcgcgaa tagaaacgta catctgaata acgaggcaga
     4501 gaatacccag cgcaatcagt actgcaccgc tagctgcaat catcagcatg gtgtggaact
     4561 gcgggtcaat ctgctggctc aaacgacggg tcatgcccat gaagcccagc gcatacagcg
     4621 gcataaaggc aacgaagaag ccgatgatcc agaaccagaa cgcgcgttta ccccaggttt
     4681 cattcagttt gaaaccgaag gctttaggcc accagtaggt catccctgcg aagcagccga
     4741 agaccacgcc gccgatgatc acgttatgga agtgcgcaat caggaacagg ctgttatgca
     4801 gaacgaagtc cgcgcccggt actgccagca gcacgccagt catcccgccc accgagaaag
     4861 tgacgataaa accgatggtc cacagcatcg cagaatggaa cacgatgcgg ccctgataca
     4921 tggtgaacag ccagttgaag atcttcaccc cggtcgggat ggcgataatc attgtggtga
     4981 taccaaagaa ggcgtttacg ttcgcgcccg cacccatcgt aaagaagtgg tgcagccaaa
     5041 cgatgaacga cagcacggtg atacagacgg ttgcccatac cagcgaggta taaccaaaca
     5101 gacgtttacg cgagaaggtt gccgcaattt cggagaacac accgaaaaca ggcaggatca
     5161 ggatgtaaac ttccgggtgg ccccaggccc aaatcaggtt gatgtacatc atcatgttgc
     5221 cacccatatc gttggtaaag aaatgggtgc ccagatagcg atccagggtc aacaacgcga
     5281 cggtaaccgt cagaattggg aaggaagcaa taatcagtac gttcgcgcac agtgatgccc
     5341 aggtaaatac tggcatcttg aacatggtca tgcccggtgc gcgcatcttc agaatggtaa
     5401 cgaagaagtt gataccggta agcgtcgtac ctataccgga tagctggaga ctccatatcc
     5461 agtaatcgac accgactccc ggactgtact ctattcccga tagcggtgga taggccagcc
     5521 agccggtctg cgcaaattcg cccacgccga gagaaacgtt aaccagaatc acaccaacaa
     5581 cagtaaacca gaagcttaag ttgttgagga acgggaacgc aacgtcacgc gcgccgatct
     5641 gcagcggaac caccaggttc atcagaccga taacgaaagg catcgccacg aagaagatca
     5701 taatcacgcc gtgcgcggtg aagatctgat cgtagtggtg aggtggcagg aagcccgctt
     5761 cgcccgccga ggcaagagcc tgctggctac gcatcataat ggcgtcagca aaaccacgca
     5821 gcaacatcac aatcgccacg atgatataca tgataccgag gcgtttatgg tcgacggagg
     5881 tcagccactc tttccacagg taggtccact taccgaagta agtgatcagg ccaacgagcg
     5941 ccagacctcc caaaataatg ccagcgatcg taaccatgac gataggttca tggaacggga
     6001 ctgcatcaag tgataatttt ccgaacatct ttattcttcc tcaacccctt taatgggcgg
     6061 attccgcgtg gctcatgtcc atgccttcca taccttcgtg tgcactgtgc tcaccttctg
     6121 gctgggtcat gtccatgctc ttaccgtgag ccataaactt gttaatcaca tcggcaaaca
     6181 agtctggttt cacgttggag aaatattcca cctggttgta ttcgctaggc gcggccagtt
     6241 tttcgaacgc agccatgtca gacatggtgt tcggcgactg tttcgctttt gcgacccatt
     6301 ggtcgaattc ggcgcgatcc ggtgttgcaa tagctttgaa cttcatgcct gagaagcccg
     6361 gcccgctgta gctggcggag ataccgtcat aagtaccggg ttcgttggcg atcagatgca
     6421 ggcgagtctg cataccggcc atggcataaa tctggctacc cagacgcgga atgaagaagg
     6481 agttcatcac ggagttggag gtcactttga agtacaccgg agtgttcgcc gggaaagcga
     6541 tttcattcac ggtagcaatg ccctgttccg ggtagatgaa gaaccatttc cagtccatgg
     6601 aaaccacttc gatggtaatg ggcttctcgt cgtgtgccag cggcttgcta ggctcaagag
     6661 cgtgagtggt tttccaggtc agtaccgcaa ggaagatgat gattaagata ggtaccgtcc
     6721 agaccacagc ttccacttta ttggagtgtg accagttcgg gctgtactta gcatctttat
     6781 tgctcgcacg atacttccag gcgaaaccaa cagccatcaa gattgcggga ataacgacaa
     6841 tcaacatcag gccaaatgcc gtcagtatca gtgaacgttg ctccagacca atctgtcctt
     6901 tgggatctaa cagcgcagaa ttacagccac tgagcaatac agtgcctgca aataatgaca
     6961 accatcccaa acttttattg tatttcctga gtctcattta acgacctcaa ttccacggga
     7021 tctggtggcg tttaaagtgt gtgggcattt tacgggaagg ttacattact gtaaacatga
     7081 ttaaattcct gttacttgga tttggctgtc aggtcacata tgttgcaaaa catatcaagt
     7141 tgattttaag aagctgttgc aaaagggcgt tataacgaaa ggggagtgca gaaggagttt
     7201 ggacgggcaa ttggtataac caatctggaa taaaacaatt gtttaacaaa taattatcat
     7261 tatggtgaca attgg
//
LOCUS       DAZGJW010000201         7219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZGJW010000201 DAZGJW010000000
VERSION     DAZGJW010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.201"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(156..1019)
                     /locus_tag="ACHKIK_004568"
     CDS             complement(156..1019)
                     /locus_tag="ACHKIK_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO9085593.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAVFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(991..2541)
                     /locus_tag="ACHKIK_004569"
     CDS             complement(991..2541)
                     /locus_tag="ACHKIK_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO9085594.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            2799..3578
                     /gene="deoC"
                     /locus_tag="ACHKIK_004570"
     CDS             2799..3578
                     /gene="deoC"
                     /locus_tag="ACHKIK_004570"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO9085595.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            3705..5027
                     /gene="deoA"
                     /locus_tag="ACHKIK_004571"
     CDS             3705..5027
                     /gene="deoA"
                     /locus_tag="ACHKIK_004571"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO9085596.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            5079..6302
                     /gene="deoB"
                     /locus_tag="ACHKIK_004572"
     CDS             5079..6302
                     /gene="deoB"
                     /locus_tag="ACHKIK_004572"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO9085597.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            6382..7101
                     /gene="deoD"
                     /locus_tag="ACHKIK_004573"
     CDS             6382..7101
                     /gene="deoD"
                     /locus_tag="ACHKIK_004573"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO9085598.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         1683 a         1905 c         1969 g         1662 t
ORIGIN      
        1 cgcgtcttat catgcctaca agtctgtgcc gaaccgtagg ccggataagg cgttcacgcc
       61 gcatccggca gtcggcgcat aatgcctgat gcgacgcttg tcgcgtctta tcatgcctac
      121 aagtctgtgc cgaaccgtag gccgtatccg gcatgtcaca aatagagcgc cggaaatatc
      181 aaccggctca ccccgcgcac ctttaacgca tcagccaacg gctcaacgtc ttccggcgtg
      241 gcgctcgccc agctttgcgc ctcgccatac acgccgtggg catgaaacgc gttcaggcgt
      301 accggaacat cgccaagtcc cttgataaac accgccagtt cttcgatgtg ttgcaaataa
      361 tccacctggc cagggattac cagcaaacgc agttccgcca gcttaccgcg ctctgccagc
      421 aaacagatgc tgcgcttaat ctgctgatta tcgcgtccgg ttaattgttg atgacattcg
      481 ctcccccacg ctttgagatc gagcattgcg ccgtcacaca ccgggagtaa tttttcccag
      541 ccggtttcgc tcaacatacc gttactgtcc acgaggcagg tgaggtggcg cagttgcgga
      601 tcgtttttga tggctgtaaa cagcgccacc acaaacggca gctgggtcgt ggcttcgccg
      661 ccgcttaccg ttatcccttc gataaacagc accgctttgc ggatatggct aagcacctca
      721 tccacgctca tggattgcgc cattggcgtg gcatgttgcg gacacatctt caggcaggta
      781 tcacactgct cgcagaccac agcgttccac agcactttgc cgtcaacaat ctgcaacgcc
      841 tgatgcggac actgtggcac gcactctccg caatcattgc agcgtcccat cgtccacgga
      901 ttgtgacagt ttttgcagcg cagattgcag ccctgcaaaa acagagccag acgactgcct
      961 ggcccgtcaa cgcaggagaa ggggataatc ttactgacta aagcgcatct gctgttcatg
     1021 gcttatcacg cgcggctggc gttccagaat acgagtgttg cgtgcggctt cttcgcccag
     1081 ccaggtggtg ttggtgcgtg aaccttcggc gcgatatttt tctaaatccg acaaacgcac
     1141 catataaccg gtaacgcgaa ccagatcgtt accgctgaca ttggcggtaa attcacgcat
     1201 tccggcttta aaggcaccga ggcaaagctg taccagtgcc tgcgggttac gtttgatggt
     1261 ttcgtcgagg gtcagaatgt cgctgatgcc ggaataataa taagcatgat gcggggcgac
     1321 agtttgcaga tgggtgatcg gatctggctc atcgccatac ggcaaacgcg cgcccggcgt
     1381 ggtgccgata tcggaactga tccccgactg tgcgtgtaac atggcgcgtt tttgccagcc
     1441 atatttcacg ggggtattgg cgacaaactc cgccagttgc gcgctgatgc gataacccac
     1501 ttcatttgcg gcggcttctt taccgtagcg cgcggcaatc ccttctttct cacacagcaa
     1561 gttaaccgct tccgccagcc catacatgcc aaacattggc acaaaacgtt cagggttaat
     1621 cagcccttct ttcaccagga agctattctc aaagaagtgt gattgttgat agaggaattc
     1681 acaccgcgca tcgatgatgg cgatctgctg ctggcagtag tgcggtagag tgcgcgtaaa
     1741 gaagtcatcc agcgattcgc tgcgctcggc aatggctttc aggttaaggc gtaccagcgt
     1801 gctgccacca cccgccagcg gcagtgagtt gtaacagctc acaatcccgt agcccccttt
     1861 tgtgaaaatt ttatcatgca ccggaccgtt ggcgatgtgc ggtttgctac attcacagat
     1921 gttcttcgcc acttccagca gcaggtcatc aggggtgatt tctggatcgt agataaaggt
     1981 caggttcggt gaaacctgct tcaactctgc atctgcacgt aatatcgcac gggtgatggg
     2041 cgaatcagac gggccgatat tggcgtgcat aaaggcgtct ggcagggttc tgtcgaggta
     2101 acgccagaaa cgttttattc gaatatcgat ctcgtcttgt gttagaattc taacatacgg
     2161 ttgcaacaac gcatccagtt gccccaggta gaccggcatc gatgtgaccg acggtacgtg
     2221 gtggtaaaga atggtcagca gagagagtgc gtcatcaaga tctttcgcgc cttccagctc
     2281 cagccattcg gaaccgttcg ccagaaaacg ggcgtaatcg ggtaagacat agcgcggttt
     2341 gtacggcgca tgaccttcaa acatatcgca gattacaccc tcatccagcg cgcggcgggc
     2401 ctcggcaggc agctgcggat aaggcagatt gttttctgct tccagtgcca gaaaatggcg
     2461 cttctgctcc gggctaagca ctgggctggt gacaatttgc tggcaacgtt gttgcagtgc
     2521 attttcatga gaagtgggca tcttcttttc cttttatgcc gaaggtgatg cgccattgta
     2581 agaagtttcg tgatgttcac tttgatcctg atgcgtttgc caccactgac gcattcattt
     2641 gaaagtgaat tatttgaacc agatcgcatt acagtgatgc aaacttgtaa gtagatttcc
     2701 ttaattgtga tgtatatcga agtgtgttgc ggagtagatg ttagaatact aacaaactcg
     2761 caaggtgaat tttattggcg acaagccagg agaatgaaat gactgatctg aaagcaagca
     2821 gcctgcgtgc actgaaatta atggacctga ccaccctgaa tgacgacgac accgacgaga
     2881 aagtaattgc tctgtgtcat caggccaaaa ccccggtcgg caataccgcc gctatctgta
     2941 tctatcctcg ctttatcccg attgctcgca aaacgctgaa agagcagggc accccggaaa
     3001 tccgaatcgc tacggtaacc aacttcccac acggtaacga tgacatcgaa atcgcgctgg
     3061 cagaaacccg tgcggcaatc gcctacggtg cagatgaagt tgacgttgtg ttcccgtacc
     3121 gcgcgctgat ggcgggcaac gagcaggttg gttttgacct ggtgaaagcc tgtaaagagg
     3181 cttgcgcggc agcgaatgta ctgctgaaag tgatcatcga aaccggcgaa ctgaaagacg
     3241 aagcgctgat ccgtaaagcg tctgaaatct ccatcaaagc gggtgcggac ttcatcaaaa
     3301 cctctaccgg taaagtggct gtgaacgcga cgccggaaag cgcgcgcatc atgatggaag
     3361 tgatccgtga tatgggcgta gaaaaaaccg ttggtttcaa accggcgggc ggcgtgcgta
     3421 ctgcggaaga tgcgcagaaa tatctcgcca tcgcagatga actgtttggt gctgactggg
     3481 ctgatgcgcg tcactaccgc tttggtgctt ccagcctgct ggcaagcctg ctgaaagcgc
     3541 tgggtcacgg cgacggtaag agcgccagca gctactaagt aagatgcttt acgcctgatg
     3601 cgctgcgctt atcaggccta cgagacgtat ctacccgtag gccggataag gcgtagacgc
     3661 atccggcaaa agccgcctca tactcttttc ctcgggaggt taccttgttt ctcgcacaag
     3721 aaattattcg taaaaaacgt gatggtcatg cgctgagcga tgaagaaatt cgtttcttta
     3781 tcaacggtat tcgcgacaac actatctccg aagggcagat tgccgccctc gcgatgacca
     3841 ttttcttcca cgatatgaca atgcctgagc gtgtctcgct gaccatggcg atgcgagatt
     3901 caggaaccgt tctcgactgg aaaagcctgc atctgaatgg cccgattgtt gataaacact
     3961 cgaccggcgg cgtcggcgat gtgacttcgc tgatgttggg gccgatggtc gcagcctgcg
     4021 gcggctatat tccgatgatc tctggtcgcg gcctcggtca tactggcggt acgctcgaca
     4081 aactggaatc catccctggc ttcgacattt tcccggatga caaccgtttc cgcgaaatta
     4141 ttaaagacgt cggcgtggcg attatcggtc agaccagctc actggctccg gcggataaac
     4201 gtttctacgc gacccgtgat attaccgcaa ccgtggactc tatcccgctg atcactgcct
     4261 cgatcctggc gaagaaactg gcggaaggtc tggatgcgct ggtgatggac gtgaaagtgg
     4321 gtagcggcgc gtttatgccg acctacgaac tctctgaagc ccttgccgaa gcgattgttg
     4381 gcgtggctaa cggcgctggc gtgcgcacca ccgcgctgct caccgacatg aatcaggtac
     4441 tggcctccag tgcaggtaac gcggttgaag ttcgtgaagc ggtgcagttc ctgacgggtg
     4501 agtatcgtaa cccgcgtctg tttgatgtca cgatggcgct gtgcgtggag atgctgatct
     4561 ccggcaaact ggcgaaagat gacgccgaag cgcgcgcgaa attgcaggcg gtgctggaca
     4621 acggtaaagc ggcagaagtc tttggtcgta tggtagcggc acaaaaaggc ccgaccgact
     4681 tcgttgagaa ctacgcgaag tatctgccga cagcgatgct gacgaaagca gtctatgctg
     4741 ataccgaagg ttttgtcagt gaaatggata cccgcgcgct ggggatggca gtggttgcaa
     4801 tgggcggtgg acgccgtcag gcatctgaca ccatcgatta cagcgtcggc tttactgata
     4861 tggcgcgtct gggcgaccag gtagacggtc agcgtccgct ggcggttatc cacgcgaaag
     4921 acgaaaacag ctggcaggaa gcggcgaaag cggtgaaagc ggcaattaaa cttgccgata
     4981 aagcaccgga aagcacacca actgtctatc gtcgtatcag tgaataacgg tatactgatc
     5041 tgatcattta aatttgaagc actgagtacg gagaacatat gaaacgtgca tttattatgg
     5101 tgctggactc attcggcatc ggcgctacag aagatgcaga acgctttggt gacgtcgggg
     5161 ctgacaccct gggtcatatc gcagaagcct gtgccaaagg cgaagctgat aacggtcgta
     5221 aaggcccgct caatctgcca aatctgaccc gtctggggct ggcgaaagca cacgaaggtt
     5281 ctaccggttt cattccggcg ggaatggacg gcaacgctga agttatcggc gcgtacgcat
     5341 gggcgcacga aatgtcatcc ggtaaagata ccccgtctgg tcactgggaa atcgccggtg
     5401 tcccggttct gtttgagtgg ggatatttct ctgatcacga aaacagcttc ccgcaagagc
     5461 tgctggataa actggtcgaa cgcgctaatc tgccgggtta cctcggtaac tgccactctt
     5521 ccggtacggt cattctggat cagctgggcg aagagcacat gaaaaccggc aagccgattt
     5581 tctatacctc cgctgactct gtgttccaga ttgcctgcca cgaagaaacc tttggcctgg
     5641 ataaactcta cgaactgtgc gaaatcgccc gtgaagagct gaccaacggc ggctacaaca
     5701 tcggtcgtgt tatcgctcgt ccgtttatcg gtgacaaagc cggtaacttc cagcgtaccg
     5761 gtaaccgtca cgacctggct gttgagccgc cagcaccgac cgtgctgcag aaactggttg
     5821 atgaaaaaca cggtcaggtg gtttctgtcg gtaaaattgc cgacatctac gccaactgcg
     5881 gtatcaccaa gaaagtgaaa gcaactggcc tggacgcgct gtttgacgcc accatcaaag
     5941 agatgaaaga agcgggtgat aacactatcg tcttcaccaa cttcgttgac ttcgactctt
     6001 cttggggcca ccgtcgcgac gtcgccggtt atgctgcggg tctggagctg ttcgaccgcc
     6061 gtctgccgga gctgatgtct ctgctgcgcg atgacgacat cctgatcctc actgctgacc
     6121 acggttgtga tccgacctgg accggtactg accacacgcg tgaacacatt ccggtactgg
     6181 tttacggccc gaaagtaaaa ccgggctcac tgggtcaccg tgaaaccttc gcggatatcg
     6241 gccagactct ggcaaaatat tttggtactt ctgatatgga atatggcaaa gccatgttct
     6301 gataatgggt gcggcttgat gccctcaccc cggccctctc ccacagggag agggtgaaaa
     6361 catgtagata aggataaaac aatggctacc ccacacatta atgcagaaat gggcgatttc
     6421 gctgacgtag ttttgatgcc aggcgacccg ctgcgtgcaa agtatattgc tgaaactttc
     6481 cttgaagatg cccgtgaagt gaacaacgtt cgcggtatgc tgggcttcac cggtacttac
     6541 aaaggccgca aaatttccgt aatgggtcac ggtatgggta tcccgtcctg ctccatctac
     6601 accaaagaac tgatcaccga tttcggcgtg aagaaaatta tccgcgtggg ttcctgtggc
     6661 gcagttctgc cgcacgtaaa actgcgcgac gtcgttatcg gtatgggtgc ctgcaccgat
     6721 tccaaagtta accgcatccg ttttaaagat catgactttg ccgctatcgc tgactttgac
     6781 atggtgcgta acgcggtaga cgcggctaaa gcactgggcg ttgatgctcg cgtgggtaac
     6841 ctgttctccg ctgacctgtt ctactctccg gacggcgaaa tgttcgacgt gatggaaaaa
     6901 tacggcatcc tcggcgtgga aatggaagcg gctggtatct acggcgtggc tgcagagttt
     6961 ggcgcgaaag ccctgaccat ctgcaccgtg tctgaccaca tccgcactca cgagcagacc
     7021 actgccgctg agcgtcagac caccttcaac gacatgatca aaatcgcact ggaatccgtt
     7081 ctgctgggcg ataaagagta attgtgtttc gctgaaaggc gaacgtcgtg ttaagccgga
     7141 gcaggagact gctccggctt tttagtttct attcatcaat agcaactgat ttatagattt
     7201 actattattt ttcatatgg
//
LOCUS       DAZGJW010000202         7203 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZGJW010000202 DAZGJW010000000
VERSION     DAZGJW010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.202"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(589..1659)
                     /locus_tag="ACHKIK_004574"
     CDS             complement(589..1659)
                     /locus_tag="ACHKIK_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFO9085599.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGSQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLFQAMNLCQIPG
                     HAGALLTTLFRHISIINGFDNPMTQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKHAGDGGNSGGLLSFTSAHIDAWRQQGYEDTMLTMEHIRKPLAARQALTRSEAVL
                     QKSQEITEGADSALRNAMAQIK"
     gene            complement(1656..2561)
                     /locus_tag="ACHKIK_004575"
     CDS             complement(1656..2561)
                     /locus_tag="ACHKIK_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO9085600.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHQKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVVLEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEERHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            complement(2558..4942)
                     /locus_tag="ACHKIK_004576"
     CDS             complement(2558..4942)
                     /locus_tag="ACHKIK_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO9085601.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHTQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGSDFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFVREAFGRMKKDYVCKDSELATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRRAALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATEKIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVQTNLSALMEERTETVTRRRRQDGVWGTLCRWANTSDWGW
                     KEYSVDVSRSVINMNKVRKEVMSLTRAYFGELQASIEQNINQPVRQEIDDFFCTFREK
                     VEQLRNTLIQSSEDHKRDQQAQEHLTERLQALNERVPELITDSKALREELETML"
     gene            complement(5160..6004)
                     /locus_tag="ACHKIK_004577"
                     /pseudo
     CDS             complement(5160..6004)
                     /locus_tag="ACHKIK_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
     gene            6328..6915
                     /locus_tag="ACHKIK_004578"
     CDS             6328..6915
                     /locus_tag="ACHKIK_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085602.1"
                     /translation="MGLDDFGLSILAGLLANRLDFSKPKGTSAQMNEVIPDVIEEPDK
                     FSLSANLPKRFKTFDVWSDLDELLKVVKEPKISVLIEEQPSTYYRLPSLVLESQITGE
                     WYVFPRGRMSFEGNGGGIQNAEVIFNQLKEENVPVGVWVIDQALLDRLDNGQECWPTI
                     RLQIVPFLAAPSSEYPWFEIQRNVAKLTEFHLTND"
BASE COUNT         1714 a         1920 c         1716 g         1853 t
ORIGIN      
        1 tattcctgga accaggtgtt cctgcagcta ccgtagaatt tgttacccaa agtctttttc
       61 tcatttttga tatttgcggt atagctgtcg actttcttac cgtttactaa aacgtcgagc
      121 ttcgtggctt caccgatagc atcgataaag tatacccacg cattcttatt cattcgtgtt
      181 ccgtcgacag gtggggccga atacttttta ccgttgatga taaaaataat atcagaatcg
      241 tactgattta gcggggtggg ggtatgaccg taaatccact catatgtggt agcaaattgt
      301 tgtcttctgg attaatcgtg cagttaatag aaacactacc gatactgaac atatcagtgc
      361 cagcaacact gttacgcttc tacaatgccg attctgcttg tgctacggtt gtacttacag
      421 ttaaaacaac agctaaaaga atgtttaaca gtttcatgag atttctcccc aaaattaaat
      481 aaattaatct taaccatcat ccaggtaata tatatctgaa aatattttca ataacaccag
      541 ataagaaaaa caccaccaca gtggcggtgg cgtttctccg gtgtgtcttt atttaatctg
      601 ggccatcgcg tttctcagtg ccgaatctgc accttcagtt atctcctggc ttttctgtaa
      661 taccgcctct gacctggtca gtgcctgacg cgccgccagt ggtttccgga tatgctccat
      721 cgtcagcatc gtgtcctcgt agccctgctg acgccaggcg tcgatatgtg ccgacgtgaa
      781 actgagcaga ccgccactgt tgccaccatc acctgcatgt ttcagccttt tccggggacg
      841 gatctcaagg attgtggtgt ccggaaaagc ctggcgatcc cataaagaac cgtcactcag
      901 atgcgtcaca atcaccatat tgcatccggc atcgaccaga ggcgttacag gggtatttcc
      961 ctgcatattt cgccatcctc ccataccacc atcaccgaac attgtcccct gaacctcacg
     1021 gggacggaac aacaggggca gcgcggctga cgcaagcaga gcctctttct gctgtccgcg
     1081 gggcagactc tggatatgct gaaaaacggc gtttttcgtg gttccgacac ccagttcagc
     1141 acgaatacag tcaataatat cctgcatgcc cccttctgtg gggtacagcg acacatacag
     1201 cggtagcccg tctgccagag catcagtatc aagataatga tccatcagcg ctgtcagggg
     1261 ctcatctgac aacaacggtt gagtcatcgg attgtcgaac ccgttgatta tcgatatatg
     1321 gcgaaaaagc gtcgtaagca gtgcgcccgc atgtccgggg atctggcaga ggttcattgc
     1381 ctggaacaat tttttcagca atgaaaagta aaccgatctg tttaccgaca gcacctgatt
     1441 attccccaga tgatcccaga gcgcctccag gcgtacggca gcttctgaca gatcgggaga
     1501 ggccgcgata atggcaccat tgaatgcgcc aatgctggcc cctgaaacca tgctgatctg
     1561 tgaaccacac tctgccagtg ccttaaccac tcccgcctga taagcgccca ccgccccgcc
     1621 gccggacagc accagccccg ttttcatttc tgtactcatg cagccccctt ctgaaatatc
     1681 tcatcgacca tactttcaga gaggcgttct gcagagcaga gacggggcat gcgaactggt
     1741 aagtgttctg cagcctgcgt ggtcgaccag atcaccggat gccgctcttc actgcaccag
     1801 tcccccagcc agttaatcgc gtcatgccac tcgttatctg cctgctcccg gccctgacca
     1861 ccaagccagt ccagagtgtt cagacgcacg gttgcactac cgccactctc ctgtgccatc
     1921 tgcgttaatg ctttatgtct gagaatatcc agtaactggt taacctggcc tgtttcaccc
     1981 tgacgtatca catcaccaaa cgcgccccag cgaagctctt caagcaccac ataacagcgt
     2041 ccggcagggg aaaatgacgc gtatcgcccg gcgctccacc atgagaatat ctgctcaaga
     2101 tgtaactgtg cccgctgaac ctggtcaaca cggtggagtt tttcttcaag atgattcagt
     2161 ttctggtgaa cctgatcggc cagggtgagt aactgctccc gcgtgtctgc cgaataatgc
     2221 gccagcctgg cagtatcact gaccagtgaa ctgaccctgt cgtttacccg ggtaattgcg
     2281 tagtttgtgg tggcaagtga cgttgtcatc tccgtcagcc agcgcaggga cgcctcaacg
     2341 ccctgtgcaa gcgaagcatt gatggcattc tgccgtgcag cgccttcgcc ggtcagcccc
     2401 tcttttaaac gccggaagaa cgtgcgctct ccggccgtgc ggatatgctc ctgtgcaaca
     2461 tcgataccgt ttgcaaaatc aaccacaaat ttttcaggaa ggcaggtgag tacctgacat
     2521 acccggttat cagcaatttg ttgaataaat ggtgaagtca cagcattgtc tccagctctt
     2581 cccttaacgc cttactgtca gtaatcagct cgggaaccct ttcatttaat gcctgaaggc
     2641 gctccgtaag atgctcctgt gcctgctgat cgcgtttatg atcttctgag ctctgaatga
     2701 gtgtgttacg tagctgttcg actttttccc tgaatgtaca gaaaaaatca tcgatctcct
     2761 ggcggacggg ctggttaata ttctgctcaa tggatgcctg caactccccg aaatatgccc
     2821 gggtcagtga catcacttcc ttcctgacct tgttcatatt gatcacgctg cggctcacat
     2881 ccacactgta ctctttccag ccccagtcac tggtgtttgc ccatcgacaa agcgtacccc
     2941 agacaccatc ctgtcgacgg cgacgggtta ctgtctccgt tctttcctcc ataagcgcgc
     3001 tcagatttgt ttgtaccgcg agccgggtct gtaactggct gactgccgga aaactgattt
     3061 ttaccgtgaa gccctcatcc tccagtcggg cattgatgcg tccggcgatc ttttctgtgg
     3121 catacagggc cgtaccacga aaacctgttt caatccccct gacgattttt tccagttcgg
     3181 gccgaaactg tgcctcagcc tcacggtgca ggctggtcac ggtcgattcg atttgcgtca
     3241 tcagttcaag ggctgccctg cgatcactga attttatttc ggggctgtcc ggatcaaagt
     3301 cccgcatcct gtcatgctga tttccggttc cgaaaatatc gtgaaagaaa gcgaatgcgt
     3361 ttctctcccg gggctgcgct gaattttctt cttccagcat ttgctgttct ttacgtttac
     3421 cgcttctgaa atagtcgttc agctcctcct ccagtgaggc acagaccttc gtcagtaatt
     3481 ccccggtttt ggtattgatg tcctccatgg ccattctgac atcaccggtc acctgattct
     3541 gacattcctc aatctcctgg atatccgcca gtaggctggt aatatgcgcc tgcagtgact
     3601 gaatgctttg ctgcagcccc tgatggcgta atgacagata ttcgctgaca ttttccgcat
     3661 tctgcatcag ttttgcggcg gcagaatcaa ccgccagtgc cgccgctctg gaatgggagc
     3721 tctgtatgac ttccgttatc agttgatcga taagtgagcc ttcccacagg tctgtcgccc
     3781 cttcagttgc cagttcgctg tctttacaga cgtaatcttt tttcatgcga ccgaaggctt
     3841 ccctgacaaa atcatcgacc caggcttcat caacaggaag ggttccgttt ttccgtaact
     3901 catgaagcgc acggttagcc aggtacgcct gacgtgagga tccgggataa acgcgggagg
     3961 cgggcagcac atcgctgttc agcattgccg gtaccttctg gcgtacggca tctgctccat
     4021 cgccattgcg gtctttctca tcaaatttat tgaccagcac aaacaggcgg ccggctgata
     4081 cgtcagcaat ggcattaagc tctttacgga cgtcttcatc cgcttttgag ttcatctggg
     4141 tgtaatccat cactgccagc accgcggaag ctttctgcag ttgatcccgc atcatcactt
     4201 ccatctgtgg ctggccggct tcattagggc cgggggtatc cagcagtgtg agtgttccct
     4261 ggcaggcatc catccccacc agatggctga attccacgtc gatcaccggc agtttctgca
     4321 cttctgcgta ttcatcaaaa ggaaagtcac tccccattgc cgcagcaaga cgaaccagat
     4381 cgtttaacga tgccagtccg gtaaagattt cctcctcccc gtggtattca tttttgagcc
     4441 agccatcatc cgtcagaata tccagtagtt cgcgggtatc cccggtttgc tgcagggtct
     4501 gtgcgacctg ctgtcctgcc ggggtggcga gtttttcctg cagtgtgatt aataaattgc
     4561 ggacaggctg agtatgttcc agatggagga ccggctcagt ttttccagga acgtgtcgga
     4621 tgagtgtcgg aacagaggtc atggggcggt tacggttggg cagaatttcc tgccccacaa
     4681 tggcgttgat ggtggttgat ttacctgctt tcatcgtgcc gacaacagcc agcaccatct
     4741 ccctgcgggc agttttgatt tgttcgccct ccagctcctc aatccttttc agtgctttct
     4801 gtttatcaaa aagcagtccg ttttcgttct tactgtcaga taacacatcc ggctcatcaa
     4861 ccatttgccg gagcagattg atgttcagtt gcaaaagtcg gtcggcttca tcacaaagca
     4921 gggcgatgtt cttttcgtgc attatttaac cttcctgaga cgcattttca tgacaaccac
     4981 ggaggaggcc atttattaca aacgatcgtt tataacgatc gatgtgatag taacgatcgt
     5041 ttaattctat taacttaaaa aacactacgc aaggggacaa cccgggtatg gctgcgtttg
     5101 catagtgttt tcatggagtt aaaaatctgt aacaacagcc tggtgcatca ccaccaggtt
     5161 cagaagaaaa tccggttcca taccgcgcgg gcaacagaga ccacggtgtc acgaacagcg
     5221 cgcagggccg tacggacaac agaggcaaca cagacgctct ccgctgtgtc aaatatccgg
     5281 tccaccgcac cgataaactg ttcacgggcc tgagagcgga cagactccgt gcgcagctcg
     5341 tcctgcagtc gggtcatcag gggactggcg gcatggtcgg gaagcgctgt catgagcgca
     5401 ctgaccagcg tatccggctc ccggccggtg cgggccgaaa cagccggaat accgcctccg
     5461 ttttttcgcg aatgttctgt gcctgtgcgg gagaaggctg aatgccggcc atatcccatt
     5521 catggcaggg ttccgttttg tcagcctgcg tcaccacaaa cagcacctgc tgatgtcccc
     5581 ggtgcaggat gtgtcgccag aaatactcat ccacagacag ggcccggtca tcggctttaa
     5641 tcagccacag caccaggtcc agttcaggca gaatgtcacg gtacagggct tcagactctg
     5701 catccctgtc ccggctctcg cccaccccgg gcaggtcagt gataaccatg ctgtgaccgt
     5761 gaccactcag acggaagcgc cgtacttccc gggtgccggc gtgaacatca ctgaccgggg
     5821 tgaccacccc ctgaaacagt gcattacaga gtgaggattt accggccccg cttttaccca
     5881 taatgccaat cacgggttcg tgactggtga gtttgcgcag atgttccagg atgtgacggg
     5941 aaagcgagta aggcagggag gagagcggtt tttcaattgc ctcaatggca tcagacggat
     6001 tcatacatgt attcccggtg aaaaaaaaca aaaccccgcg caccggaaag gtggcggggg
     6061 attcacggag ataatatctg tctgaaatta tttaggatgc aggtatatgc agttgatttg
     6121 tatctagtac agtactaaac ctaatctggc tgtccgctct gtgccaggag cagacgtcta
     6181 atatatctac ttggttctta aattcagtgc atcactgcaa gcagctcaac atcaataaat
     6241 tagggaataa ctcacaactt tgactgttac cacatggcat catagtgttt agagcatgca
     6301 taagcaccaa cgaggagttt taacataatg ggactggatg atttcggact tagtatctta
     6361 gccggacttc ttgcaaacag acttgatttc tcaaaaccga agggcacctc agctcaaatg
     6421 aatgaagtta taccggatgt gattgaagaa ccagacaaat tctcgctaag tgcaaatttg
     6481 ccaaaaagat tcaagacatt cgacgtatgg agtgatttgg acgaattgct aaaggttgtg
     6541 aaagagccca aaatatctgt tttgatagag gagcaaccat caacgtacta tcggctccct
     6601 agcttggttc tggaatctca aatcacaggg gaatggtatg ttttcccgcg aggccgcatg
     6661 agttttgagg gcaatggagg tggcatacag aacgcagaag taatcttcaa tcagttgaaa
     6721 gaagaaaatg ttccggtggg agtatgggta atagatcaag ctcttctcga ccgcctcgat
     6781 aacggacaag aatgttggcc aaccatacga ttacaaatag taccattctt ggccgcacca
     6841 tcgagcgaat acccatggtt cgaaatacaa cgaaacgttg caaaactcac agaattccat
     6901 ttgactaatg attaatagtg agccctaaca ggataatggc acactggata tgaccaccta
     6961 agcagaggca gcacaggggt aataaactat gaacttctgc ttttcgctca cagcggacct
     7021 tcagcttcgt taacacgtcc gctccgtgcc agtagcagac tagtgatcgc taggcttgaa
     7081 ttcattcgta ttagcgccaa gtggataata aatggtgaaa tccattagtc attactttat
     7141 cataagctcg ttatataaag attttagtat tcgtaattct tttctttgtt taagatgatg
     7201 aaa
//
LOCUS       DAZGJW010000203         7191 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZGJW010000203 DAZGJW010000000
VERSION     DAZGJW010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.203"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(49..753)
                     /locus_tag="ACHKIK_004579"
     CDS             complement(49..753)
                     /locus_tag="ACHKIK_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO9085603.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(890..1342)
                     /gene="moaE"
                     /locus_tag="ACHKIK_004580"
     CDS             complement(890..1342)
                     /gene="moaE"
                     /locus_tag="ACHKIK_004580"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO9085604.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1344..1589)
                     /gene="moaD"
                     /locus_tag="ACHKIK_004581"
     CDS             complement(1344..1589)
                     /gene="moaD"
                     /locus_tag="ACHKIK_004581"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO9085605.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1582..2067)
                     /gene="moaC"
                     /locus_tag="ACHKIK_004582"
     CDS             complement(1582..2067)
                     /gene="moaC"
                     /locus_tag="ACHKIK_004582"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO9085606.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVTALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2070..2582)
                     /gene="moaB"
                     /locus_tag="ACHKIK_004583"
     CDS             complement(2070..2582)
                     /gene="moaB"
                     /locus_tag="ACHKIK_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO9085607.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2604..3593)
                     /gene="moaA"
                     /locus_tag="ACHKIK_004584"
     CDS             complement(2604..3593)
                     /gene="moaA"
                     /locus_tag="ACHKIK_004584"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO9085608.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3581..3725)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3990..4898
                     /gene="yvcK"
                     /locus_tag="ACHKIK_004585"
     CDS             3990..4898
                     /gene="yvcK"
                     /locus_tag="ACHKIK_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO9085609.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5090..7111)
                     /gene="uvrB"
                     /locus_tag="ACHKIK_004586"
     CDS             complement(5090..7111)
                     /gene="uvrB"
                     /locus_tag="ACHKIK_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO9085610.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         1716 a         1926 c         1822 g         1727 t
ORIGIN      
        1 agaaaaaaaa tcccgacaca tggccgggat attgatggtg aaaaagaatt aacggcggtt
       61 gccaaaaatt cgcagcaaca tcaggaacag gttgatgaag tccagataca aggttaacgc
      121 gccaagaatg gaatatttgc gcaggttcga cgtgtcgcgg gtatcaatct gctcacccat
      181 atttttcaat ttctgagtgt catacgccgt caatccgaca aagacaatca cgccgatgta
      241 ggtaactgcc cacatcaatg cttcgctttt cagccagaag ttgaccagcg atgccagcac
      301 aatgccgatt aacgccataa acagcatatt gccaaagcca cttaaatcgc gcttcgtggt
      361 gtaaccgtac aggctcattg cgccgaacat cccggcagta acgacgaaag tactggcgat
      421 agaagcagcg gtatagacaa tgaatatact ggaaagcgta agacccgtca gcgccgaata
      481 aagcataaag agcatcgtcg ttacacctgc gctcagcttt tgaatcatcg ctgataacac
      541 aataaccaat gctaattgcg cgatgatcag accgattaaa aagacacggt tagtaaacaa
      601 cagctccatc acggccgcgg aattagccgc ataccaggca acaaatgcgg tcagcaacaa
      661 gccaacggtc atccagccat agacttgcgc catataagtt tgcaagccag cccggggttg
      721 tacgattgaa tcagaacgtg ggaatctgtc catgacgatc tcctgaagat ataaggaata
      781 tcttaaggat actgcaaaat gatgaggctg tgcatcgacg cagcgtaaac gcatgtactg
      841 agcggtgaaa ttgccggacg cagcggtgcc ttatccggct aacaaaaaac taccagcgtt
      901 ttgccgcctg ctgatcgctc tcccgagctt caacccagcg gtcgccttcc ggcgtggctt
      961 cgcgcttcca gaacggtgcg cgggttttga gataatccat aataaactgc ccggcttcaa
     1021 acgcactgct gcgatgcgca ctggtgacac cgacaaaaac gatttcatcg cccggccata
     1081 attccccgat gcggtgaatc acagtgacgc gccccagcgg ccagcggtta cgcgcttcat
     1141 caacaatttc tgccagtgct ttttcagtca tccccggata gtgttcgagg gttaatgctt
     1201 tgacgctgtc gcccaggtta tggttgcgca ccttaccagt aaaggtgact accgcaccgt
     1261 cttcgtcacg ctccgccagc cacgggtact cttctcctac gctgaacggc tgcggaccaa
     1321 caacaatttt ggtttctgcc atcttaacct ccggttaccg gcgggaagaa agctacttcg
     1381 tcgccgtcag tcagcggatg gtcaaaactc accagcgtct ggttgacggc agccagtaat
     1441 ttgccatctt ccagcgccag cgcccagcga tcgctctgcg cagccatgtg ctggcgtaac
     1501 gcttcaacag ttgggaaatc cgcagccact tcggttgcat ctgttcccac caactcgcgc
     1561 acctgggcga aaaaaagaac tttaatcatc cgcttccacc ttaaagtcac ccgacttgcc
     1621 gccgctcttc gccagcaaac gtaccggacc aatcaccata tctttttgca ccgctttgca
     1681 catgtcataa atggtcagcg ccgtcacgga ggccgcggtt aatgcttcca tttcgacacc
     1741 ggttttcccg gtcaggcggc ataaggtttc tatacgcacc cgattgtgct ccggctcggc
     1801 ctgtaaattg acttcaactt tgctgagcat cagcggatga cagagcggga tcagatccca
     1861 ggtgcgtttt gccgcctgaa taccggcaat acgcgcagtg gcaaatacgt cgcctttgtg
     1921 gtggcgacca tcaataatca tcgccagcgt ctcgctgcgc atggtgacaa aggcttccgc
     1981 ccgcgcttca cgcacggttt ccgctttggc ggagacatcc accatgtgcg cttcgccagc
     2041 ggcgttgata tgggtcagtt gcgacatact tatttcttca aatgtggatg gaaattacac
     2101 ggacgcgtac gggcatccag ctgcggcgcg atgatatttt cccatgcggt acggcacgct
     2161 ttggtcgaac ccggcatggc gaaaatcagc gttttgttgg cgacgcccgc taccgcacga
     2221 gattgcaacg tggaagtgcc aatctcttca aacgacaaca tacggaacac ttcaccaaaa
     2281 ccttcaattt cacggtcgaa caacggcagc aatgcttcgg gagcctgatc accttccgtc
     2341 aggccagtac cacccgtaat caataccact tgtacatcgt cgctggcgat ccacgcagat
     2401 acctgagcgc gaatagcgta gcggttttct ttcacaatgg ctttatcgac aacgtgatgg
     2461 cccgcttctt gcgccgaatc gcgcagatag tgaccggagg tatcgtcttc ttcaccgcga
     2521 cgattagaaa ccgtaagaat agcaatacgg gtcgggataa attcagtgct tacctgactc
     2581 atctgatctc tccttttgac gttttagccg ccaatgtacg ataagttttg cgtaataccg
     2641 gtgttgtttt gatgcaggaa atgggtctgt ttcttctccc gcagcgccgc tgaaatacgc
     2701 gcttccagcg cctgttgctg ggtatcgtct tccagcagat cgcgcaggtt aacgccgcct
     2761 tcaccaaaca ggcagagatg gagtttacca atggaggaaa cgcgcaggcg gttgcaagtg
     2821 gcgcagaagt ctttttcata cggcatgata aggccaatct ctccggcgta atctggatgg
     2881 caaaagactt gcgcgggacc gtcgctgcgt tgacgtaatt ggtggatcca gccgcgacgc
     2941 agtagctcgt cacgcagaac ctgaccagag atgtgatgct tacggaagag ctcgctgccc
     3001 tcgcccgttt ccatcagttc gatgaaacgc agctggatag ggcgatgctg gatccagttc
     3061 agaaaggtgt cgagctggtg atgattaaca tcacgcatca gcacggtatt gactttgacc
     3121 ttctcaaaac cggcctcaaa tgcagcatca atccccgcca tgacctggtt gaatttatcc
     3181 tgcccggtaa tagcgtgaaa ctggcgggcg tccagactgt cgacactgac gttaatgcca
     3241 gtaagtcccg catcgcgcca gttcgccaca tcgcgttcca gacggtaacc attggtggtg
     3301 accgcaatct ggcggatagc gtcgttttcc cgcacagcgg cgatgatatc ggtaaagtcg
     3361 cggcgtaaag acggctcacc gcctgtcagg cgcacttttt cggtgcccag actggcgaag
     3421 gcgcgcgtaa cccggcgaat ttcatcgacg gtaagaaagc ctttattggt gacgccgctc
     3481 ggtttgtagc catccggcag gcagtaggtg caacgaaagt tacacacatc ggtaatcgac
     3541 aggcgcaagt agtaaaactt acgcgcaaat gcatcagtca gttgtgaagc catgtacacc
     3601 tttccagata cgggaggcga agtcatttct tccttcgccc tggtggcaat ctttacgagc
     3661 attgtcacgg ccaaagcacc gtatcagttg acccaggtgc agaggctaga gtgtttagtg
     3721 gttatgccga tactagcgtg taaatgagat ttttaccatc cacactttcg ctatgtaatc
     3781 atgtatatag cgtcatgatc gtgctatttc gctacagaat aagggaaaat ggcggtttta
     3841 ctttgatata catcatcagg gaggcatagc gtgcatcgtc ctttagaggt agaagaaaat
     3901 agtttgtatc ctcaatattg gcaggttaat tgctgtttcc ccgcaatttg cgctactgta
     3961 gcgcggataa tctgactcca ggaaactata tgcgcaatcg tacgctggct gatcttgatc
     4021 gtgtcgttgc tctcggcgga gggcatggac tgggacgcgt tctctcatca ctttcgtctt
     4081 tgggttctcg tttaacgggt atcgtcacca ccaccgataa tggtggctcg acggggcgta
     4141 ttcgccgttc agaaggcggc attgcctggg gcgatatgcg caactgcctc aaccagctga
     4201 taacggaacc gagcgtcgcc tccgcgatgt ttgaataccg ttttggtggc aatggcgaac
     4261 tttccggtca taatctcgga aacttgatgt taaaggcgct ggatcacctt agcgtgcggc
     4321 ctctggaagc catcaattta attcgtaatc tgctgaaagt ggatacgcat ttgattccaa
     4381 tgtcagagca tcctgttgat ctgatggcga ttgacgatca ggggcatgaa gtttacggcg
     4441 aggtcaatat cgaccagtta actacgccga ttcaagagtt attgttaacg cctaatgtac
     4501 ccgcaacgcg tgaggcggtt cacgctatca atgaagcgga tctcatcatt attgggcctg
     4561 gcagttttta taccagcctg atgccaattc tgctgctgaa ggaaatcgcc caggcattac
     4621 gccgcacgcc agcgccgatg gtttatatcg gcaatctggg gcgtgagttg agtttacctg
     4681 cggctaattt gaagctggaa agcaagctgg caattatgga gcagtatgtt ggtaaaaagg
     4741 tcattgatgc ggtcatcgtc gggccaaaag tggatgtctc ggcggtgaaa gagcggattg
     4801 tgatccagga ggtactggag gccagcgata ttccgtatcg tcatgaccgc cagttgttac
     4861 ataacgcgct ggaaaaggcg ttacaggctt taggttaacg gaaatccgaa ggaaaattcc
     4921 ggcttcctat tgaagacaaa gtgcgcgttg tttatgccgg atgcggcgtg aacgccttat
     4981 ccggcctaca aaccgcgcaa attcaatata ttgcggagaa aatgtaggcc tgataagcgt
     5041 agcgcatcag gctgttttcc gtttgtcatc agtcttcttc gctatcctgt tacgatgccg
     5101 cgataaacag ctcacgcagc tgatgcaact ggtcacgaat ttgcgccgct tcttcgaact
     5161 ccagattctg cgcgtgttgc atcatcaacc cttccagctc atggattttc tgctgcaacg
     5221 ctttaggcga catatccatc ggcacattat ccggctcaac aatcggtcgc gattttcctc
     5281 tgcccttcgc tttggtttta gcaatgttct gccccaacgc caggatatcg accactttct
     5341 tgttcaagcc ttgcggcgta atgccgtgtt cctcgttgta cttctgctgt ttctcgcggc
     5401 gacgttcggt ttcgccaatc gctttcgcca ttgatggggt gatcttatcg ccgtagagaa
     5461 tcgctttacc gttaacgtta cgtgccgcac gaccaatggt ctggatcaac gaacgttcgg
     5521 aacgcaggaa gccttctttg tcagcgtcga ggatcgccac cagcgaaact tccggcatat
     5581 ccagaccttc acgcagtaag ttgatcccta ccaatacgtc gaactcaccc agacgcaagt
     5641 cgcggataat ctccatacgc tcgacggtgt cgatatctga gtgaagataa cgcacgcgct
     5701 caccgtgttc ttcgagatat tcggtaagat cttccgccat tcgcttggtc agtgtggtga
     5761 ccagtacgcg ttcgttaatt gccgctcgct gacgaatctc cgaaagaaga tcatcaacct
     5821 gtgtcgccac cggccgcact tcgataatcg ggtcaagcaa tccggttgga cgcaccacct
     5881 gatccaccac atcgccgccg gatttttcca gctcgtaatt acccggcgtc gccgaaacat
     5941 agatggtttg cggcgctaat gcttcgaact cttcaaactt aagcggacgg ttatccagcg
     6001 ctgatggcag gcggaagccg tactccacca gtgtctcttt acgcgcccgg tcaccgcgat
     6061 acatgccgcc aatttgtgga atggtgacgt gagattcatc gaccaccagc agcccatcag
     6121 cagggaggta atcaaacagc gtcggcggtg gctcacccgg tccacgaccg gagaggaagc
     6181 gcgagtagtt ttcaatcccc gaacagtagc ccagctcgtt catcatctcc agatcaaact
     6241 gggtacgctg ggtcagccgc tgctcttcca gcagtttgtt gttttccaac agcactttgc
     6301 gtctggcggc cagctcttct ttgatctcct ccatcgcctg tacgatgcgc tcgcgcggtg
     6361 tgacgtagtg cgttttcggg tagatggtaa aacgtggaat agtggaaaca atctgcccgg
     6421 tcagcgggtc aaataacgac aatcgttcca cttcctcgtc aaacagttcc acgcgaagtg
     6481 caatgtcatc cgattctgcc gggaagatat ctatcacctc gccacgaacg cggaaagtac
     6541 cacgctggaa tgcttgatca ttacgagcgt attgcagctc cgccagtcgg cgcagaatcg
     6601 cgcgctgatc gataatcata ccgaccgtga gatggagcat catcttgaga tataaatcag
     6661 gatcgcccag accataaatc gcggaaacag acgccaccac aaccacatca cgccgctcca
     6721 gcatcgcttt ggtggcggac aaacgcatct gctcaatatg ttcgttaacc gaggcatctt
     6781 tctcaatgaa agtgtcggaa ctcggtacat aggcttccgg ctgatagtag tcgtagtagg
     6841 aaacgaaata ttccaccgcg ttttccggga agaactcttt catttcgcca tacagctggg
     6901 ccgccagcgt tttgttgggc gcaagtacca tggttgggcg ctgaaggtca gcaatgacat
     6961 tggcaatggt gaatgttttc ccggagccag tcacgccaag taacgtctgg tgcgccaggc
     7021 catcttccag cccctcttcg agacgtcgaa tcgcctctgg ctgatcgcca gaaggtttaa
     7081 aagcggaatt cagtttgaac ggtttactca tgagtcgcta cctgaaggag ttgggcgggc
     7141 aggtatgtaa ttttactcgt cgtacttaat tatgccaaca aattatactg g
//
LOCUS       DAZGJW010000204         7176 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZGJW010000204 DAZGJW010000000
VERSION     DAZGJW010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.204"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..196
                     /locus_tag="ACHKIK_004587"
     CDS             <1..196
                     /locus_tag="ACHKIK_004587"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085611.1"
                     /translation="IFIFHLVNNFFSILARFSQYLVRVETELQRGSNPVGTPGTARLF
                     SGRVPSAFDANYGFDSHIHS"
     gene            357..1484
                     /locus_tag="ACHKIK_004588"
     CDS             357..1484
                     /locus_tag="ACHKIK_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFO9085612.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
     gene            1490..2281
                     /locus_tag="ACHKIK_004589"
     CDS             1490..2281
                     /locus_tag="ACHKIK_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFO9085613.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(2317..3242)
                     /locus_tag="ACHKIK_004590"
                     /pseudo
     CDS             complement(2317..3242)
                     /locus_tag="ACHKIK_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            complement(3387..4736)
                     /gene="lysC"
                     /locus_tag="ACHKIK_004591"
     CDS             complement(3387..4736)
                     /gene="lysC"
                     /locus_tag="ACHKIK_004591"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO9085614.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDIA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     regulatory      complement(4828..5024)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            5261..6910
                     /gene="pgi"
                     /locus_tag="ACHKIK_004592"
     CDS             5261..6910
                     /gene="pgi"
                     /locus_tag="ACHKIK_004592"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO9085615.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALVGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDGKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         1825 a         1715 c         1848 g         1788 t
ORIGIN      
        1 aatctttatt tttcatttgg ttaataattt tttctcaatt ctggcacgct tctcgcaata
       61 tctcgtacgc gttgaaactg aattacaaag ggggtcaaac cccgtcggca caccaggaac
      121 ggccaggctg ttttccgggc gtgttccgtc agcctttgac gccaattatg ggttcgattc
      181 ccacattcat tcctgaaatg aatactggtt ttaacttatt aaataaaaag tgagggattg
      241 cgcagtcagc gatccccgat aacgtcgccg ataaagatgc cgggtaacag gcaaaaggga
      301 attcccctgt cgcttcgtgt ctgcttccgc gacggctgtt aagcaaggaa tagataatga
      361 ttaaaaaaat aagcgtaaga aaagatcagc tggcattact ctccagaaac ggcgattact
      421 acaaagtgct acatgcaggc gagcatcttt taccctggtt aaatacgcca gaagtcttgc
      481 tgattacgct cgatggcagc gaagttcccg acgtgctggc ggattatttg cgccgctttc
      541 agcctgattg ggtggagaaa tattgcctgg tggcagactt gtcggagatt gaagccgggg
      601 cgttgtatat ggacggtatt ttgcaggaga tcctgccgcc atcaacgcgc cgtttgtact
      661 ggcgtgtaga ggatgatttg acactggtgc ggatgaatac gcagcaagtc caggtacaaa
      721 cggaggtgat gaatgcggta ttgcaaccac ggcgcaaagg ggcagttaaa ggccgcgatg
      781 caatactaac ggtgcaggtt ccggcctggc atgtaggcgt gctgaaaatc gatggtgaaa
      841 cgcaggcgct gttaccgccg gggctgaccg catactggaa aattaaccat ctggttgacg
      901 ctgaagtggt tgacacccgc ttgcaggtac tggaagtcag cgggcaagaa atcttaacga
      961 aagataaggt caatctgcgg attaacctcg ccgctaactg gcgctacagc gacgtgttat
     1021 tggcatttag ccagttaacg aagccgattg atcatttgta tcgggaatta caatttgccc
     1081 tgcgtgaagc ggtaggtacg cgtacgttgg atgaattgct ggaagataaa caggtcattg
     1141 atgacgttgt cagcgaacag gtgaaatcgc gcatgttgcc atttggtatg gagattgcct
     1201 cgttaggggt gaaagatatc gtcctgcctg gcgatatgaa aaatatcctt gcgcagctgg
     1261 ttgaggcgga gaaatccgcg caggccaatg tgattcgccg acgtgaagaa acggcagcga
     1321 cgcgttcgtt actcaatacc gctaaagtca tggaaaacaa cccggtagcg ttacgcttaa
     1381 aagaactgga aacgctggag agagtggctg aacggatcga taacatctct gtatttggcg
     1441 gtctggatca ggttctgcat gggttggtta atatcaaagg gtgagtaaca tgccattaaa
     1501 tggagtcagt gctgcgatgc gagaaagggt atcgcagcag ttaaaagaaa ttgaacggcg
     1561 gtatgatgtg aaagtactgt acgcctgtga atcagggagc cgcggctggg gatttgcctc
     1621 gcccgacagc gactacgatg tacggttttt gtatgttcac ccgctggaat ggtatctgcg
     1681 agtagagtca ccgcgcgatg ttattgagct gcccatcgac gacgagttag atgtttccgg
     1741 ttgggagtgg cgtaaggcgc tgggattatt gaaaggcgcg aacccgacgc tgatagaatg
     1801 gctggattct ccggtggttt atcaacaaga tgaagaaacg ataacggcgt ttaaggcaat
     1861 ggtcccaatg tggttttctc cgctgcgtgc gcgctggcat tactattcga tggcgcagaa
     1921 aaacttcccc ggatatttgc agggcgacga agttcgactg aaaaaatact tttacgtgct
     1981 gcgtccgctg ctggccgtgc gctgggtcga agcgggtaaa ggcgtaccgc caatgcgctt
     2041 tgctgagtta ctggcgggaa gcgaacttga tgcgccgtta cgtgcagaaa tagacgaatt
     2101 actggaacgt aaacagcgcg caggagaagc ggaatatggt ccgcgccgtc cattgttaca
     2161 tgcgtttatt cgtgcggaac tggcgcgtgg ggaaattcca ccgctgttac cggatagcag
     2221 agaaggggac gttaaggaac tggatagctt aatgtaccag acggtaatgc gccgggcata
     2281 aagcccggcg ttatttcacg ccatcagctc ttattattat tgtgtattcc cgctgatttc
     2341 attctgtata agcgttgcaa tatcgtcatt cgttgccgct tgtgaactaa atatcatctt
     2401 ctcacgcgaa tacaccgcaa acttatctga caggttcgaa gcaaacacct tgcgggcatt
     2461 ttctgccagc aaaccggtga cgatatcatg gtggttttta tccacttttc gggcaatatt
     2521 tcccgcctct ttgtccttac tataccgttc attggtgctt tcccagctta atgccgctga
     2581 ggttgttttt atcatgactt ctgtttggta gcctgcatct ttgagttttt taatgtttgt
     2641 aacggcgtcg cggcattacg aaatgttcct tcgacaacaa tgttgagttt actgactatc
     2701 gctctctcaa ttgcacgttc gaccgtttcg ccggaaaatt tcgctgtatg ggtaacaaaa
     2761 tcatccccat aattttggta gatataatta aaatcagggt gtagaaatcg aaaatcatca
     2821 ccgttaataa tcattatatt atcttttgtc tctcttttgg ctaattcaat cagagatgat
     2881 ttacctgctc cgggttgccc acctaaaaca aaaccaatgg gatgctgttg cggtgtgact
     2941 tcatgatttg tcagacgctg ccaaatagaa tcaaaagatg ctttattttc tgaatgcgta
     3001 gtattgttga attcatgctg atattgagtt cttacctgct gaaggagaat cttaccttgt
     3061 gtaaaatcaa caagttcagc gggggtagaa taaagataat cgcgatagct gactaatcgc
     3121 ttgagagcat cgcctgaatc ctgatgcgac gaaactgagc aattatcaat gatatttgat
     3181 aaaaacgcga tgcactgact ggctccgttt aaaagtgcat ctttttccat taaagtagcc
     3241 attaacactc ctgttaaatg atatttttcg gtgattataa tactgttcta caggagtgat
     3301 tttgcttttc agatattatt taatctatgt tttcagtgag gatggcgtat aacaatcggt
     3361 gagcgttata cgccagtagg gaaatattac tcaaacaaat taaaatgcag cttctgcacc
     3421 acctgctcgg catcttcgcc gggcaccagg aagcacaggt tatggctgga tgcgccgtaa
     3481 caaatcatgc gaatgttgaa cggttccagt acgccgaata cctctttgcc aacgccgcag
     3541 gcttttgaca ggtcattgcc aatcaacgcg accagcgcca gaccttcttc tacttccacc
     3601 cggcacagtg cggaaagctc catcagcaga gattgcgtca gcaacgtatc gccagtggag
     3661 gttgaaccgg tggtatcaag ggttaatgcc acgctcactt ctgacgtggt gattaagtct
     3721 accgaaatat tatgccgcgc gaggatgccg aaaacttccg cgaggaagcc gcgagaatgc
     3781 agcatattca ggctgtgcaa agtgagcaga gtctgattgc gacgaagcgc cagcgcgcgg
     3841 aacagcggcg gattttcagt tttattgcac accagcgtac cacctgcgcg tgggtctttg
     3901 ctggagccga caaagaccgg gatatcgctg cgtactgcgg gtagcaacgt tgccggatgc
     3961 agtacttttg caccaaaagt tgccatctct gccgcttcgg caaacgcgat ttcatcaatg
     4021 cgttttgctg cggaaactac gcgtggatcg gtggtgtaga tgcccgggac gtcggtccag
     4081 atatcaacac gagatgcgtg taaagcctcc gccagcaagg ctgccgtata atcgctgcct
     4141 ccacggccaa gcgtcgttgt acgaccttta ttttcgctac cgataaatcc ctgggtgatc
     4201 actaagcctt cattgagacg tgggagcagc tgcagcgcgg ccagttccgc cagcgcggct
     4261 atatctggct ctgcacgacc aaatcggtcg ttggtacgca tcactttacg tacatcaaac
     4321 cactgtgcct gaacatcgcg ttcgcgcagg atctcaacaa acagcagggt cgacatcagc
     4381 tcgccgtggc tgaccagctc atctgtcagc gccggagacg ttgccagcgc cgccgcttct
     4441 gccagaacag taatgttctc cagcagacgt tcaatctctt cacggataac gttcgggtaa
     4501 cgcagacgtt ccagaatggc aaactggatg ttgcggatag cgtcgagttt ttcgaatcgc
     4561 tcgccaggtt ccagtccttc agctaaagcg accagcagat tagtgatacc agcagaagcc
     4621 gagaggacaa ctaaacgcac gttggcatca gaaagcacaa tatcagcgct gcggttcatg
     4681 gcgtcaaaat cagctacgct ggtaccgcca aatttggaga caacaatttc agacataact
     4741 acctcgtgtc aggggatcca ttttcagcct tggcacaagg gaagagcgga agacgggtgg
     4801 gcgcagagcg atacttcgct actattttca cccagaagtg ctccaccact tgcgaaacgc
     4861 ccgactgcga acgcttctgg tgacaaccca ggggattcag cccctgtagc cgatgatgaa
     4921 cgtggccagc cgttcaatca cctcggcgat gcaccccctc aggtgttatc acaggactgg
     4981 ctcctccaac accgttactt gggcaacgcg cctcttctgg cctgcgctag cgcaggtagt
     5041 acatttataa ataaagggtg agcggggcgg ttgtcaacga cggggtcatg cggatttttc
     5101 atccactcct ggcggtcagt agttcagcta ataaatgctt cactgcgcta agggtttaca
     5161 ctcaacatta cgctaacggc actaaaacca tcacattttt ctgtgactgg cgctacaatc
     5221 ttccaaagtc acaattctca aaatcagaag agtattgcta atgaaaaaca tcaatccaac
     5281 gcagaccgct gcctggcagg cactacagaa acacttcgat gaaatgaaag acgttacgat
     5341 cgccgatctt tttgctaaag acggcgatcg tttttctaag ttctccgcaa ccttcggcga
     5401 tcagatgctg gtggattact ccaaaaaccg catcactgaa gagacgctgg cgaaattaca
     5461 agatctggcg aaagagtgcg atctggcggg cgcgattaag tcgatgttct ctggcgagaa
     5521 gatcaaccgc actgaaaacc gcgctgtgct gcacgtagcg ctgcgtaacc gtagcaatac
     5581 cccgattttg gttgatggca aagacgtaat gccggaagtc aacgcggtgc tggagaagat
     5641 gaaaaccttc tcggaagcga ttatctccgg tgagtggaag ggttataccg gcaaagcaat
     5701 cactgacgta gtgaacatcg ggatcggcgg ttctgacctc ggcccgtaca tggtgaccga
     5761 agcactgcgt ccgtacaaaa atcacctgaa catgcacttt gtttctaacg tcgatgggac
     5821 tcacatcgct gaagtgctga aaaaagtaaa cccggaaacc acgctgttcc tggtagcatc
     5881 taaaaccttc accactcagg aaaccatgac caacgcccat agcgcgcgtg actggttcct
     5941 gaaagcggca ggtgatgaaa aacacgttgc gaaacacttt gcggcgctct caaccaatgc
     6001 caaagccgtt ggcgagtttg gtattgatac tgccaacatg ttcgagttct gggactgggt
     6061 cggcggccgt tactctttgt ggtcagcgat tggcctgtcg attgttctct ccatcggctt
     6121 tgataacttc gttgaactgc tttctggcgc acacgcgatg gacaagcatt tctccaccac
     6181 gcctgccgag aaaaacctgc ctgtactgct ggcgctggtt ggcatctggt acaacaattt
     6241 ctttggtgcg gaaactgagg cgattctgcc gtatgaccag tatatgcacc gtttcgcggc
     6301 gtacttccag cagggcaata tggagtccaa cggtaagtat gttgaccgta acggtaacgt
     6361 tgtggattac cagactggcc cgattatctg gggtgaacca ggcactaacg gtcagcacgc
     6421 gttctaccag ctgatccacc agggaaccaa aatggtaccg tgcgatttca tcgctccggc
     6481 tatcacccat aacccgctct ctgatcatca ccagaaactg ctgtctaact tcttcgccca
     6541 gaccgaagcg ctggcgtttg gtaaatcccg cgaagtggtt gagcaggaat atcgcgatca
     6601 gggtaaagat ccggcaacgc ttgactacgt ggtgccgttc aaagtgttcg aaggtaaccg
     6661 cccgaccaac tccatcctgc tgcgtgaaat cactccgttc agcctgggtg cgttgattgc
     6721 gctgtatgag cacaaaatct ttactcaggg cgtgattctg aacatcttca ccttcgacca
     6781 gtggggcgtg gagctgggta aacagctggc gaaccgtatt ctgccagagc tgaaagatgg
     6841 caaagaaatc agcagccacg atagttcgac caatggtctg attaaccgct ataaagcgtg
     6901 gcgcggttaa tcatcgtcga tatgtaggcc ggataaggcg ttcacgccgc atccggcaac
     6961 cgatgcctga tgcgacgcgg tcgcgtctta tcaggcctac aggtcgatgc cgatatgtac
     7021 atcgtattcg gcaattaata catagcacga ttgattaaat aaccttaata acaatgccga
     7081 cgttatgtcg gcattttttt atcagataaa tccccttgtc tgtgatttaa cggaaatcat
     7141 accatgaggt taatcctaaa atagattttt aatcgt
//
LOCUS       DAZGJW010000205         7139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZGJW010000205 DAZGJW010000000
VERSION     DAZGJW010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.205"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..296)
                     /locus_tag="ACHKIK_004593"
     CDS             complement(<1..296)
                     /locus_tag="ACHKIK_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9085616.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRV"
     gene            complement(293..2905)
                     /locus_tag="ACHKIK_004594"
     CDS             complement(293..2905)
                     /locus_tag="ACHKIK_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9085617.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVHRHFSSLEADARKTASTV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISD
                     SFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGELGRFMSVARVAA
                     GGGVAVLAALAAAAWQAEQADRALLRSLILTGGAAATTTAELWKMAGVISDEAGGGIR
                     QAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALAS
                     LNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQLDKAATPVEKIW
                     DDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLALAEFIQSAWETT
                     KNVPGVGALFGELAEENRVFIEKTKRDELALRKSIAERDARIRQGEMGYINRSRATGV
                     SKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEAELRTLEKHSGA
                     NEKISRQRRDLWKAESQYAVLKEAATKRQLSWQEKSLLAHEKETLEYKRQLADLGDKV
                     EHQKRLNELAQQAVRFEEQQSAKQAAISAKARGLTDRQAQRESEAQRLRDVYGDNPQA
                     LARVTGALKQTWADEDMLRGDWLAGLKSGWGEWAESATDSFSQVKSAATQTFDGIAQN
                     MAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASSGTA
                     IEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEG
                     GYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGINGQAGPQLMKAVYDMARKGAQDELR
                     LQLRDGGMLSGSGR"
     gene            complement(2886..3299)
                     /locus_tag="ACHKIK_004595"
     CDS             complement(2886..3299)
                     /locus_tag="ACHKIK_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO9085618.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGDGGRD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
     gene            complement(3326..3748)
                     /locus_tag="ACHKIK_004596"
     CDS             complement(3326..3748)
                     /locus_tag="ACHKIK_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO9085619.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYIKFVSDAEQQETTKHD
                     VVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEQTGKAEATEPVTSKKHSKAS"
     gene            complement(3762..4514)
                     /locus_tag="ACHKIK_004597"
     CDS             complement(3762..4514)
                     /locus_tag="ACHKIK_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9085620.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VTAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(4522..4917)
                     /locus_tag="ACHKIK_004598"
     CDS             complement(4522..4917)
                     /locus_tag="ACHKIK_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO9085621.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(4914..5492)
                     /locus_tag="ACHKIK_004599"
     CDS             complement(4914..5492)
                     /locus_tag="ACHKIK_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9085622.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(5504..5857)
                     /locus_tag="ACHKIK_004600"
     CDS             complement(5504..5857)
                     /locus_tag="ACHKIK_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO9085623.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(5869..6264)
                     /locus_tag="ACHKIK_004601"
     CDS             complement(5869..6264)
                     /locus_tag="ACHKIK_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO9085624.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(6306..>7139)
                     /locus_tag="ACHKIK_004602"
     CDS             complement(6306..>7139)
                     /locus_tag="ACHKIK_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO9085625.1"
                     /translation="SRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRR
                     RRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAANNITQSG
                     GTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVRDKLDTRRGS
                     HSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGNTQARGL
                     RTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDEFVSVQL
                     A"
BASE COUNT         1438 a         2327 c         1705 g         1669 t
ORIGIN      
        1 tcaacgcgca acatcccgac ccgcgcagac cacccggcac aggtcacctt tatctgccgg
       61 tatgcatagg gtggcttcca caaaaatgcc ttccagcctc cgtgctctgc caggaacgct
      121 tccagatgcc gggcctcctc ccgggtcacg gaaagcgtca cacggtatgt tttcaggtca
      181 gcattcagcc ctgccgccat acgctgtgag tacccgtcac caaaacgcac ttcacgcacc
      241 gatggctgcg agttcacctc catatccggc ttcactttcc agcgaaatgt tttcatcgcc
      301 cgctccctga taacataccg ccatcacgca actgcagccg gagctcatcc tgcgccccct
      361 tgcgggccat gtcatacacc gccttcatca gctgcggccc cgcctgtccg ttgataccgt
      421 cgttctgaat caccacgtga ttgttctgat taaaattaat accttccgcc cgccgcatct
      481 gcgccggact tccggcacca cccacataac caccttccgc atagccgcgc atcagacggt
      541 aaagattccc cacacctatc cggctggttg cctctttcgt gaaaacaaac tccccgcggt
      601 gaactatccc cgcaggctca tatttgccgc ccgtccccgt aaatcctccg gtcgcgaaat
      661 ggaagttcgc cgccgcagcc tcaatggccg tccccgagga agcagatgca ccaccaccga
      721 aagcaccgcc aatggcgctg ccgatacgcc cgacaatgcc caccatggcc tgtttaagca
      781 ggatttctgt catcatggac agcaccgaac gggtgaatcc ccgccagtct gcctctgcac
      841 cggtcagcat cgccgccata ttctgtgcaa taccgtcaaa ggtctgcgtg gcagcacttt
      901 taacctgcga aaaactgtcc gtcgcacttt ccgcccactc cccccagccg gacttcagcc
      961 cggccagcca gtcaccgcgc agcatgtctt catccgccca tgtctgtttc agtgccccag
     1021 tgacccgggc cagcgcctgc ggattatcac cgtacacgtc ccgaagacgc tgcgcttcag
     1081 actcccgctg cgcctgacgg tcagtgagac cgcgggcttt tgcgctgatg gcggcctgct
     1141 tcgcgctctg ctgctcttca aaccgcaccg cctgctgtgc cagctcattc aggcgtttct
     1201 ggtgttcaac cttgtcgccc aggtcagcca gctggcgttt gtactccagc gtctctttct
     1261 catgggccag cagggatttt tcctgccagg ataactgccg tttcgtggca gcctctttca
     1321 ggaccgcata ctgactttcc gccttccata aatcacggcg ctgccgactg attttctcat
     1381 tcgcaccgct gtgtttttcc agcgtcctga gctcagcttc aagggcaagc agagcctctc
     1441 ttgcctgctc ctcttccctc tccccggcag agcgcgtttt cggtgatgta tgctttttac
     1501 ctgtcagctc ttcagccaga cggctgacgg cttcctgctg cccaggacct ttgctgacgc
     1561 ctgttgcacg cgagcggttg atgtacccca tttccccctg gcgtatacgc gcatcccgtt
     1621 ccgcaatgga ttttctcagc gccagttcat cgcgttttgt tttctcaata aatacgcggt
     1681 tctcttctgc cagttcacca aacaacgcac caacgccggg cacattcttt gtcgtttccc
     1741 aggctgactg aataaattca gccagcgcca gatccccctg cacaagcagc agcttcactt
     1801 gttcaacggt tccggccacc acgtcagtga tcagactgag tgcccccagt gtatgatcac
     1861 ctatccatgc ccatgcgtca gaagtccagg ttttaacatc gtcccagatt ttttccaccg
     1921 gcgtggccgc tttatcaagt tgctccagac gtgcattcat gacatccgca aacagggaca
     1981 tcgcctccgt caccgcagcc tgtttacctt tcgtgcgctc aagctcatca atatggcgta
     2041 actgggaaac gctcaggaag ttatactgct gattcaggga ggccagcgcc ttcaccggat
     2101 ctgctgcaat cccttcaaag gctttttcca ccttcccggc atcgtccccc accgtctgca
     2161 gccatctctg agaggtttcc cccatgatcc gtagctgccc ggcggtatat ttcccgcttt
     2221 ctgccagacg ggccagattt tctgccgcct gtctgatacc accaccggct tcatcgctga
     2281 tcaccccggc cattttccac aattctgccg ttgtggtggc agccgcccct ccggtcagga
     2341 tcagtgaacg caataaggcc cggtcagcct gctctgcctg ccaggcggcg gcagcaagcg
     2401 cggccagtac ggcaaccccg ccacctgccg ccacacgggc caccgacata aatcgtccca
     2461 gctcaccggc attccgggca ttttcagcca gtgcatttgc cgtatctgac agcgactcct
     2521 ctgatgattc agaggcatcc ctgatcccga gaagttcctc cttcagcagg gtaagcaggc
     2581 tgagcggtcc accgaatgaa tcgctgatct gccccccctg ctgcagcatg ataaggaagg
     2641 gattctgacc accggcaagc tgagtgacaa tatccgtgaa ctgtgcgggc agtgtgcgca
     2701 tggcagcctt atactgtccg actgatatcc cggctttttg tgcagccagc gcctgtcggc
     2761 tcagcccctg ttcaacagta ctggcggttt ttctggcatc cgcctccaga ctggaaaaat
     2821 gacggtgtac ccgggccatc tgttcatcaa accgggccgc atccagactc aaatcaataa
     2881 ccagatcacc cgctggctgg gacatatctc acacctccgg aaatccccgc tgaagccatc
     2941 attaatgcaa catcatcctc gctgacatcc accacatccg cagaaggtga aatatcgcgc
     3001 cctccgtccc ctccgaaccg gacgcctccg gcaactcctg tcgctttctg catcagcatt
     3061 tcttcctcgt ccggcatctc cgtctgctct tcctcacacg ctggagcaag cagactgaaa
     3121 tccgccggat gcatatccgg atcgccaaaa aacaggctga gtacggcgta catcagcccg
     3181 gaaaaatgag cgtccagttg ggtatcctga aaataatgcg tgcagtaaaa acgtcgccag
     3241 tcggcatatt cggtggatgt catcccggca agcatggcgc gccagtcggg tctccccatc
     3301 tctcgcgcca gtttcaggac aaagttcagc tcgccttcga atgctttttt gatgttaccg
     3361 gctcagtcgc ttctgctttc ccggtttgtt caggatcggc atcgtgccgg ttatccagca
     3421 tacctgaaag ataaagcacc cggttcgttg cctgattcag tgcatcagca ggccatccca
     3481 gcatcacttc acggcggatc tgctgcatct ctgtctccgg agaggccaga gtgcctttga
     3541 gggaatggga atgccatagc gacatcgcca caagcaggga tgccgtttcc agatatcgct
     3601 ggttaatgtg cacgacatca tgcttcgttg tctcctgttg ttctgcgtct gaaacaaact
     3661 ttatataatc aaaccgctgc agcgcagaca attcggaaag cgtgacggac acaccgttat
     3721 attcaaattg ttctgttttc agaaacatgt attacctccg tttaccctgc agcgcccgct
     3781 tcagtaacgg tgacttcagc cactgcggcg aactgaccat ttccgctcac cacagggatc
     3841 tgcaccttac ctgtcgccac gccgtttacc gtaattgtca tatctttcac actaatggtg
     3901 gctttcgacg gatcggcgga aaccgctctg aacgtcttgt cggttgcact ttccggctca
     3961 aaagaaaccg tcagggtggt tgttttccct tttgccaccg taccggatgt cggcgtcacc
     4021 ttaatcgcag tgaccggcgt aattttgctg cgttcttccg ctacagaagg tttgcccacg
     4081 ttggtcactt tcaccgtgcg ggtgatcact tctttcgccg tcacggcctt accgatactg
     4141 ctgacccagc cacggaacac atccaccgtg ccatttggga aacggatttt ataggcccgc
     4201 acatccccgc tttcaaacca gcctataagc cctttctgac cttcttctcc cggtttccag
     4261 gccagcgtaa aactggtatc tcctgcagac ttctgcccct gcccggtcgc ggtccagtcc
     4321 gcgtcttcat catccaggta gttatcatcg tagggttctg ccgtcatctc gcccggcgtc
     4381 agatccttca ccttagccag tcgctgccag tcatcgtctg acaacgggtt tgcataagca
     4441 tcacccttgc cgttgtaaac ccacagagtg gtaccggcac cttttaccgg ctcaagggga
     4501 tttggtgttg ccatatcgtc ctcacatctc gtatgtaatg gaataagtca gatctgcaga
     4561 actccataac gccatatcgt catcacgacg atactcatag ccctgcgtaa ccatcgtggt
     4621 aatcagtcct gccagtgccg ggatatcgct catcaccgga taaatccggg actccatcca
     4681 cgcatccagc tctgaatccg gcacctgagc aggcaggaaa acttcaatat gcagctccgc
     4741 ctgccaggta tcgctgtcca gctcttcgcc cgtgtattca gcgccggtga gataaacggc
     4801 aactgccgga aaatccgcct catcaaaaac agcggggcga ccatcaaaaa gcgtcgcccc
     4861 ggtgtcatgc ttctccagtg catccagtac ggctgcacgg agttcagtat gtttcatcgc
     4921 tttattacca ttctcagttg atgctgcagc gcatagccca gctctttcgg aagacgttca
     4981 cgccgtatcc gttcaatatt ctgtttaaac gccgtggtaa gcggcaccgc catcgggatt
     5041 ttcaccacat caatggggta acggtttttc ccggccacac gctgcatgac atgccaccgc
     5101 ccatttttca gttgctgaat aaacgcgccg ggaatacgac ggtttcccac cacaagcacg
     5161 ctgccgccac ctttcaggga tgaacgctgc ccctttttac gacgcctgcg tcgggacagg
     5221 acaacccgcg cattccccag cttgattacg ggcaaatccc cccggttaac cttgattctg
     5281 gcctgcggat ttttaaccgt ggcccttttc agcctggccc tttcctttac cagtttccgg
     5341 cgtacctttg tctcacgggc aacctgtgac gcagactgcg atatcgcgga tgaagcaacg
     5401 cggttaatgg ccattgctga cgcaccgggc accgccgttt tgctgatacg gctgaggttt
     5461 tcaacggcct gctcaagacc ttttatggcc atacatcccc ctttcagcgg cgacggttaa
     5521 cggcaggcgg cacgccacgc ccaagccaga gatgacagct tccgccatca tccggcgaaa
     5581 tccggtctat ccagaagttt tcctcaccga tggtcagcgt gtctccacgc cgcagctgcc
     5641 gcacatcatc agtccggaca aacagggacg ggctggagcc ttcaacgcgc acgccctgtc
     5701 cggcatagct gatattttca gggtcatcaa aaacaccacg tattactgcg ccggactgct
     5761 caccggatgt catggtggct gacgttccca tgtacccgcg tatcgtttca tcggcgcggg
     5821 caatggcagc atcgaacagg ttatcgaaat cagccacagc gcctcccgtt attgcattct
     5881 ggccaggcca cgttctgtca tttcggctgc cacaccggca gagacacgaa acgccgttcc
     5941 cggcagcaca aatgccacag gttcatcccg cgtggcgtga agtgcatcgg tatgcagcgt
     6001 caccagtgcc acaaccgtga ccagagcagc cgtatcagtc acggtatccg tctgtgctga
     6061 taccacctca ttttcatgtc cggtcagcgc attttccggg ctgacagacg tgtcctgacc
     6121 ggctgcgtca tccgtgtcat caagctcctc ttccagctct gccacacgga gcgccagttc
     6181 ttctttcgtc cccgtcaggc tgacatcacg gttcagttgt tcacccagcg agcggagacg
     6241 ggcaatcagt tcatctttcg tcatggactc atccacagag aaacaatggc cccgaagggc
     6301 catgattacg ccagttgtac agacacgaac tcatcagggt cagccagcag catcagcggt
     6361 gctgactgaa tcatggtgaa ctctcgcgcc ggatcgccgg tagtcaccca gtttttcggg
     6421 taacgggcag aggcgttaat gccttcgcgc tgtgcgtccg catcctgaat acagccatag
     6481 gtacgcagac cgcgtgcctg agtgttcccc agcaccatcg tgttgtccgg caggaagttc
     6541 tttttgacgc cgttttccac gtactgtccg gaatacacga cgatggccac atcgccatac
     6601 attcccttat aagacaccgc tttgcccagg tcttttaccg ctgtctccag ctcggaatga
     6661 gagccgcgac gggtatccag cttatccctg acggctttga aggaacggaa cagcgcccat
     6721 cctttcggat caaacacgat gatattcacc acgccgctgg cgttcagcgc gtaggcttcg
     6781 atatcgtcgg tcgggtcata cgtggacttg tcacgcttgc tccactccgt gccaccggac
     6841 tgtgtgatgt tgtttgccgc actgcggccc atatccacct caaccggatc aaaggcttca
     6901 ccggtcatgg tgtatttgcc cttaagcacg gcagaaactg cctgcatctc ttcgacctgg
     6961 gcaatggcca gctcttcgtc acgcatgttc tgcatgatga tgcgacggcg gcggtaagcc
     7021 gggtccgcca gattctgcgg atcttcatcc ggcaggcgac gcagggtcat ctgcggattc
     7081 acctcatgct tcggcttgac atatcccggc gtaaattcag aggtggagcc gccacggga
//
LOCUS       DAZGJW010000206         7122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZGJW010000206 DAZGJW010000000
VERSION     DAZGJW010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.206"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(181..1374)
                     /gene="tyrB"
                     /locus_tag="ACHKIK_004603"
     CDS             complement(181..1374)
                     /gene="tyrB"
                     /locus_tag="ACHKIK_004603"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO9085626.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKSRELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1609..2706)
                     /gene="alr"
                     /locus_tag="ACHKIK_004604"
     CDS             complement(1609..2706)
                     /gene="alr"
                     /locus_tag="ACHKIK_004604"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO9085627.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVDLSFCNI"
     gene            complement(2759..4174)
                     /gene="dnaB"
                     /locus_tag="ACHKIK_004605"
     CDS             complement(2759..4174)
                     /gene="dnaB"
                     /locus_tag="ACHKIK_004605"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO9085628.1"
                     /translation="MAGNNPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4257..5240
                     /locus_tag="ACHKIK_004606"
     CDS             4257..5240
                     /locus_tag="ACHKIK_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO9085629.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5406..5648)
                     /gene="pspG"
                     /locus_tag="ACHKIK_004607"
     CDS             complement(5406..5648)
                     /gene="pspG"
                     /locus_tag="ACHKIK_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO9085630.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5782..6819)
                     /gene="dusA"
                     /locus_tag="ACHKIK_004608"
     CDS             complement(5782..6819)
                     /gene="dusA"
                     /locus_tag="ACHKIK_004608"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO9085631.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNTQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
BASE COUNT         1804 a         1887 c         1765 g         1666 t
ORIGIN      
        1 aaaaaaacag gataaggaat tccagataat tgggataagc ttaacaataa aagaactaac
       61 aacgtttatt atctctcacg tagaacgatg gcatcaaaaa aatcatctat aaagaaagag
      121 tgaaagcgac gacagtttaa tttcactgca ggctgggtag ctccagcctg ctttcctgca
      181 ttacatcacc gcagcaaacg cctttgccac acgttgtaca tttgccgtat ttaacccggc
      241 gacacacatg cgaccgctgg cgatgagata gacaccaaat tcttcacgta gtcggtcaac
      301 ctgagcggca cttaaaccgg tataactgaa catgccgcgc tgattaagca gataatcgaa
      361 attgcgttct ggcatctctg tgcttaatac cttcaccagt tcctgacgca ttgccagaat
      421 gcgagtacgc atctcttcta cttccgccag ccagctggct ttcaatgcct cgtcattcag
      481 cactgcagcc accacctgcg caccaaaatt cggcgggctg gagtagttgc ggcgaactgt
      541 tgctttcaat tgccccagta cgcggccagc ggcttcggca tcttcacaca gaacagaaag
      601 tccgccgacg cgctcgccgt aaagggagaa aattttcgag aacgaattgc tcaccagagc
      661 gggtaatcca gcgctggcaa tggcgcgaat agcgtaggca tcctcttcca taccggcacc
      721 aaatccttga taggcaatat cgaggaatgg aataagctcg cgggatttga gaatttcaat
      781 caccgcatcc cactgatcat tagtgagatc ggcacccgtt gggttgtggc aacatggatg
      841 cagcaacaca atactgcggg caggtaatgt tttcagcgtc gccaacaggt cattaaagcg
      901 cacgccgtta gtcgcttcgt cataccaggg gtaagtactc acttcgaatc cagccccggc
      961 gaatattgct acgtggtttt cccaggtagg atcgctgacc cagacgcctg attccgggaa
     1021 gtagcgtttc aggaaatccg cgcccacttt caatgcccct gagccgccaa gggtttgaat
     1081 ggttgctacg cgctgttgtt gcagtactgg atggtccgca ccaaacagca gcggcgcaat
     1141 ggcatggcga tagctgttaa gcccttccat cggtaaataa agcgaagcgc catgaggctg
     1201 cgcattcagg cgcgcttccg cctccgccac ggcttgcagt tgtggaataa ttccgtcttc
     1261 gttgtagtac agaccgatac ttaaattcac tttgtcgctg cgagggtctt ctttaaaacg
     1321 ctccataagc gtaagaatcg ggtcgccagc gtaggcgtca actttttgaa acacgcgatg
     1381 gttctccagg tttacgggca ggtggttaaa acacaataaa ccggaagaag gcgaagatcg
     1441 agtggatgtt caggagcgaa cggcaattag caacagagtg agactcatga caaacgtaca
     1501 tccgccagag gcacgacctt tataagagca tgaaagaaca tcaacttatt gaatttttag
     1561 gattttattg gccggataag gcattcacgc cgcatccggc acagacaatc aaatattaca
     1621 gaacgataaa tccacgtatt tcatcgcgac ccttgaagtc aggcgcgtaa taagttcgta
     1681 agcgcttact ttcgtcattt cagcgatacg ttctacgggc aaaccttcgc cccataaaat
     1741 gaccggatcc ccggctttgt cctgcgcttg tggacctaag tctacgcaga tcatatccat
     1801 cgccacgcgc ccgacaatcg gtacttcgcg accgttcacc agcactggcg taccggacgg
     1861 cgcggcgcgc ggataaccat cgccatagcc catcgcgact acgccaagac gggtatcacg
     1921 ttcgcttacc caggttccac cataaccaac aggttctccg gctttatgct cacgcacggc
     1981 aatcaggctg gaggttagtg acatcactgg ctgacagcca aaatcggcac cggtggagcg
     2041 atcttccagc ggcgagacgc cataaagaat gatgcccggg cgcacccagt caaaatgcga
     2101 ctgtggccac agcagaatgc cacccgatgc ggcaatggaa cgttgaccag gtttgccttc
     2161 gcaaaaggta ttaaagatag cgagttgttt ctcggttgcg ccacattttg gttcatccgc
     2221 gcgcgcaaaa tggctgacga tattcaccgg ctgacgaacg tttttgcact gggtcaggcg
     2281 atgataaaac gcctcagcct gttccggcct tacgcccaga cggtgcatac cggtatcgag
     2341 tttcatccag acggtaaccg gctcgtccag gctagcctct tccagcgcag ccagctgttc
     2401 ttcgttatgc acggcggtat gaaaatgttg cgcagaaatc gtcggcagat ctctggcatc
     2461 aaaaaagcct tcgagtaaca gtacaggttt ggtgattccc cccgcacgca gtcgcagagc
     2521 ttcttcgaga cgggctacgc caaaggcgtc agcatcgggg agcgttcgcg cggtctcaag
     2581 aagaccgtga ccataagcgt tcgctttcac caccgcaacc attttactgg caggggccag
     2641 ttcacgaaga cgttgcaggt tgtgtcgcag agcgcggcgg ttaatcacaa cagttgccgc
     2701 ttgcatttgt gttccttgat aagtgtttgc tttaattacc taattcataa aataattatt
     2761 attcgtcgtc gtactgcggc cccgcatagt tgtcgaagcg cgaccattga ccgttaaagg
     2821 tcaggcgtac cgtcccgatt gggccgttac gttgtttacc gataataatt tccgcgatgc
     2881 cttttaaatc actgttttcg tgatacacct catcacgata gataaacatg atcaagtccg
     2941 catcctgctc gatagagcca gattcacgca ggtcggagtt gaccgggcgt ttgtcggcac
     3001 gttgttccag agaacggttc aactgggaca gcgccaccac cggcacgttc agttctttcg
     3061 ccagtgcttt cagcgagcgg gagatttctg caatttccag cgtacggtta tcggaaagcg
     3121 ccggtacgcg catcagttgc aggtagtcga tcatgataag cccgatgccg ccgtgttcac
     3181 gggcaatacg gcgtgcgcgg gaacgcactt ccgttggcgt caggccggag gagtcatcga
     3241 tatagatatt gcgtttttcg agcaaaatac ccatggtgcc ggaaatgcgc gcccagtctt
     3301 catcatcgag ctgcccggta cggattttag tctggtcaac gcgcgacagc gacgccagag
     3361 aacgcatcat aatctgttct gaaggcatct ccagcgagaa gataagtact ggtttatcct
     3421 gcaacatcgc cgcgttttcg acgaggttca tcgcaaatgt tgttttaccc atcgacggac
     3481 gcgcggcgac gatgatcaaa tccgacggct gcaagccagc ggtttttttg ttgagatcgt
     3541 cataaccggt gtttactccg gtaacgccat cgtgtggctg ctgaaacaac tgctcaatac
     3601 gcgccacggt tgcgtcgagc acatcggcga tgttcttcgg cccttcgtct ttgtttgcac
     3661 gactttcggc aattttaaag acgcgggatt cagcaaggtc cagcagatct tcgctggtac
     3721 gcccctgcgg atcaaaaccg gcttcagcaa tctcattcgc aaccgagatc atctcacgga
     3781 caacggcacg ttcacgcacg atgtcagcat aagcactgat gttcgccgca cttggcgtat
     3841 tttttgacag ctctgccaga taagcaaaac caccgacgct atcgagttgc ccctggcgtt
     3901 ccagcgattc cgcaagggta atcagatcga taggactacc gctttcctgc aaacgcgcca
     3961 tttcagtaaa gatatggcgg tgtgggcggg tgtaaaaatc gtctgccacc acacgctcgg
     4021 ctacatcgtc ccagcgttcg ttatccagca ttaaaccgcc caacaccgac tgctccgctt
     4081 cgatcgagtg cggaggcact ttcagcccgg caacttgtgg atcgcgttcg cggggttcag
     4141 cctgctgttt gttgaagggg ttatttcctg ccatagtgaa tggagttacc gagataaaga
     4201 atgggtcgaa actttaccat atgaagcagc ccctgacgat acgttctgga ggacacatgg
     4261 caacacgaat tgaatttcac aagcacggtg gcccggaagt acttcaagcc gtagagttca
     4321 ctcctgccga tccggcagag aatgaaatcc aggtcgaaaa taaagccatc ggcatcaatt
     4381 ttatcgacac gtatatccgc agcggccttt acccgccgcc atcactaccc agcggattag
     4441 gtaccgaagc ggcaggcatc gtgagtaaag tcggcagtgg tgtaaagcat attaaggcag
     4501 gcgatcgtgt tgtctatgcg cagtcggcgt taggcgctta cagctctgtg cataacatta
     4561 atgcggataa agcggcgatt ctgcctgcgg caatttcttt tgagcaagct gcggcatcct
     4621 tcctgaaagg cttaacggtt tattatctgc tgcgtaaaac ctatgaaatt aaacccgatg
     4681 aacagttcct gttccacgca gcggctggcg gcgttggctt gattgcctgc cagtgggcaa
     4741 aagccctggg cgcgaaactt atcggcaccg taggaaccgc gcaaaaagcg cagagcgcgc
     4801 taaaagcggg cgcgtggcag gtaattaact atcgtgaaga gaatctggtc gagcggttaa
     4861 aagagatcac cggcggtaag aaagtgcgcg tggtgtacga ttccgtgggc agagacacct
     4921 gggaacggtc gctggattgc ctgcaacgcc gcggcttaat ggtcagtttt ggcaactcat
     4981 caggtgcggt taccggtgtg aacttaggca ttctcaatca aaaaggctcg ttgtatgtga
     5041 cacgcccttc cctgcaaggc tatatcacca cgcgggagga attaaccgag gccagtaatg
     5101 aactgttctc tttgattgcc agcggtgtga ttaaggtcga tgtcgccgag cagcagaaat
     5161 atccgctgaa ggatgcgcag cgtgcgcatg agattctgga aagccgggcg acgcaaggtt
     5221 ccagcctgct gattccataa aagaaatagg gcttccacct gggaagccct ttctttttat
     5281 agttcggctg tatgtagggt acagcacgat gaatctgtta gaggcgcaat agtgacagat
     5341 ttgattatca attcctattt tgttctaagg ataaaacctt aggttgtgat catccgcaca
     5401 atcccttagt aacgccagcg gtcataacgc tgatatttag gcacttttgg tgctttaatc
     5461 gccttaataa cccacaccac cgcaatcgcc agtagtaacc acggcagcaa cttaatcatc
     5521 aatgccagca taccgccgag gaacataatg gccgtcgcca caaccagcgc ggcgataatg
     5581 cccagcaacg aaacgccggt gaccatcagc atgacaaaaa agccaatcac aaaaagtagt
     5641 tccagcatga tgctctccca aatatgaaat ctcttgctgg cattacaaga atcatgccaa
     5701 aaataatcta ttgatttaac agcaaaacgc cccgcgacgg tgcgcagggc gtggtgaatt
     5761 tgactacttt ttggtgaaaa gttaacgctt atccgccacc agtttgagcg cgtgttccag
     5821 cacattaatg tctgcacccg ctttatgggc attttcactt aaataacgcc gccactgccg
     5881 cgcgccagga ataccctgga acaagcccaa catatgccgg gtaatatggc cgagatacgt
     5941 cccctggctg agttcacgct caatgtacgg atacatggcg cgcactaccg ccaccggatc
     6001 ggcatcggta tccgaggaac caaagatctc ccggtctacc gccgccagaa tacccggatt
     6061 ctgatacgcc tcgcgcccga ccatcacgcc atccatatgt tgcaggtgtg ctttggcctc
     6121 ttccagcgac ttgataccac cgttaatcga cattgtcaga tgcggaaagt cacgcttcag
     6181 ttgatacaca cgcggataat cgagcggcgg gatttcacgg ttttctttcg gacttaaccc
     6241 cgaaagccag gctttacgtg cgtggatgat aaacatctca cactcgcctt tgccggaaac
     6301 ggtgttgatg aaatcgcaga gaaattcata gctgtcctgg tcatcgatgc caatacgcgt
     6361 tttcaccgtc actggaatcg acaccacatc gcgcatcgct ttcacgcagt cggcaaccag
     6421 ctgcgtatta cccatcagac acgcaccaaa catgccgttc tgcacccggt cagacgggca
     6481 gccgacattc aggttgatct catcatatcc acgcgcttct gccagcttcg cacactgtgc
     6541 cagcgccgcc ggatcgctac cgccgagttg caacgctacc ggatgttctt cttcactgta
     6601 cgccaggtaa tcacctttac cgtgaataat cgcccctgtg gtcaccattt cggtatacag
     6661 caacgtattg cgggaaagca gacgcaagaa atagcggcaa tgtctgtccg tccagtcgag
     6721 cattggtgca acgctaaacc gaccactcca gtgaacgtca gttttttcag gcattgcgct
     6781 ggtttggttg attttttgca tttcagaatt accgtgcatt ttcaaatgta gagattattt
     6841 tatcgatata tcatggggtt atgttattca gcatcactat tcaggaggct caatagcggg
     6901 gtactatacc ataacaacag gaagcgcctg tctcattgca aaagaaaatt gagatcctct
     6961 caaggcatga agctctcacg aagtgatgga aataatctta ttagccgtta actttgttaa
     7021 ggccaatgat aaacaatcca ggttcgacga taaataaaaa atcacacatt aaactctggt
     7081 gatatatctc cctgctaata gcattgatag agaaaaaaga ac
//
LOCUS       DAZGJW010000207         7059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZGJW010000207 DAZGJW010000000
VERSION     DAZGJW010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.207"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(184..2967)
                     /locus_tag="ACHKIK_004609"
     CDS             complement(184..2967)
                     /locus_tag="ACHKIK_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO9085632.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVHDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(3024..5396)
                     /locus_tag="ACHKIK_004610"
     CDS             complement(3024..5396)
                     /locus_tag="ACHKIK_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO9085633.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSVVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5434..>7059)
                     /locus_tag="ACHKIK_004611"
     CDS             complement(5434..>7059)
                     /locus_tag="ACHKIK_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO9085634.1"
                     /translation="PFWLRKNNKTSVLLIIIILAMILGVVKIQVWLNDWNNDFFNALS
                     QKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIRWREWLTDYYLNRWFADKNYY
                     FTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQSLSMLITFTVILWQSAGTLS
                     FTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRPLNVEKQRSEATFRTNLVQHN
                     KQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQRWLDYWQNIYSRSLSVLPYFL
                     LLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDELAELAAVIDRLYEFHQLTEQ
                     RPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSPGKWLLLKGYSGAGKTTLLKT
                     LSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICKALPLPVDDKSLSEVLHQVGL
                     GKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWIFLDETTSHLEEQEAIRLLRL
                     VREKLPTSGVIMVTHQSGVWNLADDICDISAVL"
BASE COUNT         1828 a         1617 c         1572 g         2042 t
ORIGIN      
        1 aaggtgtttt atgctgttat ttttatgcgc attctgtgtc tcctgaatta tcacgtaaaa
       61 atcagacctt aaaatatcac tattagtact tgattattat tttgaacgca tttataaaat
      121 tattacataa aaatagcgaa tattgctaaa atccccgcca acgatgtgtt gacggggctg
      181 ttattatttt ggcaataata ctccggtata agtatttacc ggatgagaaa gatattgttt
      241 aacggcagtg ttaacattct ctaccgtcat ttgtttcaac aattgctcct gctcagtcca
      301 tgctgcagga tcgtcatatt gaataagact atttacaata gtgttcgcta attgttgaac
      361 gctacgctgt tggatatcga ggctgcgctg aacgttttgc tggtattcat tcagttcttg
      421 ctcactgatc cctttagcca gacgcttaac catcacttca ttcgctaacg ttaacagttc
      481 atcatgtcgt tctggttgac aagtaaaagc cagcaaatga ctgatatctt tggcctgagg
      541 atcaaccgag aggcgagaag aaacgctgta tgctccagat gcctgttcac gaatattaac
      601 acgtagatct tttgccagtg cgacgttaaa agcatcgagc gccatacgcg tcgccagatt
      661 aacaggtgtc cgggaatcat aacgcttcca ctgtgaaacc tgtgccacag gttcattttg
      721 ttcttttaca gtaaccgatg cgttgtccgt cgcgcgagtt aatggtttac ctgcggctaa
      781 tggcgaatca gagtgtttga ttgatcctaa gtaacgcgta attaacgcca cgagtttgtc
      841 ttctgagaca ttaccgacaa tgacaaacgt gatatccgct ggagatgaaa acaactggcg
      901 atcggcagcc agcgcatctg cggcagtaaa ctgtacaatc tgattttctt gcagtaattt
      961 cgtgcggtca tcagcatagc gcgtctcata catctgctgg gcgaatttct ccgctggacg
     1021 ctggtcgagc gttttcaacg cctgaatttg agcattttgt agcgatgccc aaatattgtc
     1081 gttaatcgtg ctgtgggtga ttcgctggtt aatcaactga aaaccaggtt cagggttatt
     1141 agtccgcgcg ctaacggaga gcaacgtatt catgccactg actttactgc tcatggttac
     1201 cgaattttcc gcactccagc gtttcaggct ggaagaggag agctcaccaa cgccgcttcc
     1261 gcttactgct ttatttgcca gcgcgataag tgatttttgc tgcgcaggga aacttaaatc
     1321 gcctttattc gatacggcag taatttgtag cttttgctct tcacccgcgg attttgccag
     1381 aataaccctg gcaccattgg aaagtgttaa tgatgtcaga ttctcagcca gcgtttcttt
     1441 gctgctaatt tccgcctgtg ggtcagcgtc tactgttaac gataaatttc tgcctgggaa
     1501 gatgtaagcc gccagctttt tgttggcgta ctctttttcc agcgccagga tagccgcagg
     1561 agacaatgct tttttggcgg caagctcatt gtttaccatt tgctcccaaa atgcgtcctg
     1621 gttctttctt aactgctgcc atttttccgc cagactttgc acggtaattt gctgccacag
     1681 acgtttcgaa agttgatatg tctcttccgg cgacaagaac ggcgtattat ttaatgagct
     1741 ggatgccagg cgactggtca gcatacgtaa atcacgttca gcttgctgat caaccgcatt
     1801 tttcagccag gtgaggcggg tagatttaac atcatcgagt tcttcagcag aaaaaccatg
     1861 ctgatcaata gttgccaact ctgccattaa tgcattcgca gcatcctgca tattatcgtc
     1921 tcgtgcattt acacggaaaa acagcgactg ataatcgggt gcaattttaa cgctgcgcgc
     1981 agtgccgcca gaaatagtct tcaactcgcc cgactgtatg cgttcctgca gacgttgatt
     2041 gaacagctga actaacatgc tccattcagc ttgttcgata aagctttgct catcgtgcac
     2101 ttgtaccatt ggcaggcgat aatagagtgc gatgccgttc acccggtttt ctttatcatt
     2161 aatgatatta aagcgcaggt ggttttcggc ttttgtcggc cagacgcgat tttcagctgc
     2221 tttgttagcc ggaagcttac ttaaattatc ctttatcagc gccagcgctt ctttactgtc
     2281 gatatcgcca accacgataa aggtcatatt atttggttga taccagcgtt gataaaattg
     2341 gcgcaattgt gccggtgtga ccgtggcgac agtatccatc aggccgatag gttcacggtc
     2401 taaattacgg gtattagcca gcaggaaagg gcggcgcgcc tgagaggtgc gccatttcgc
     2461 atcctgatgg gcacgccatt cctcagtaat tacgccacgt tcagcgtcta cttcgagttt
     2521 ttcaaaggtt gcggcattac tccattcact gaagattgcc atcacttgtt gcagattttg
     2581 tttctgcgta gtcggcaaac tcacctgata caccgtttcg tcatagctgg tataggcatt
     2641 aacatcgcga ccaaaacgca ggcccattga ctcaaatgtt tcgatgactt tattacccgg
     2701 ccatgttttt gtgccgttaa acatcatatg ttctacaaaa tgagccacgc cgcgctcatt
     2761 gtcttcttcc tgcaatgaac cggtatgaat ttgcagccat aaatttacct gatcttttgg
     2821 atgagcatgc ggataaatca tatatcgcaa gccattgccc agttgcccgg taattaactt
     2881 ttcatcctgc ggtaaggcgg cggcaatcag ccgcccgggg agcaacagag ttgccactaa
     2941 cgtcagtaag aaacagaggt ttctcataat tatctccatg cgaaaaccgg gcgaatttac
     3001 ccggttaagt aaaatccgaa ctattaaaat ttcatgctga catcaagcca gaaagtacgg
     3061 ccactggcgt aggtcgccac accggtattc gttgtatcaa cagcggtttt gctatccaat
     3121 acattgagaa tatcggcact gatcgtcagg ttttgttgtt tcagaaattg tggcgtccag
     3181 gacaagcggg tgtcccaggt caggctgcta tccagttttt cgtcaacata ctgcttgtaa
     3241 tctgaatatt cgctgatgta ttgcgcattt gtcttaccca ggataatgcg agctttacgc
     3301 gcttcttgcc aggccagcgt attcgcccac actaacccgc tcggttgatg cgtgaaatcc
     3361 atgtttaagg agatctttaa tgggttatta aaatctgcca ctggaacgct atcgtaagaa
     3421 accagattac cgttataaac cacctggtta tcaccggtat tgctctcctc ataaccattg
     3481 tttaacgaca agttgccctt gctcttgata tagctaaaga caatttgcgg gttaatatct
     3541 acctggctga tatgcagggg ttcggccagt tcaaaactga ggttgaacga atgcgttttt
     3601 gttttgccgt cgttgttata ttcagtaatg gtggttttag tcgcgctgtc ggtacgactg
     3661 cttttgctga tttgatcatg cgcttcacgg taaacatagt ttgcgcgcgc aataacgttc
     3721 ttaccgattt tctgctgcaa tcccattgcc agttcatcgt tataaggcgt tttcaaatcc
     3781 tgataacgcg tcagggtttt attgcctgat accgattccg tccagctatt gcggatatca
     3841 cgtaatccca tatcaagaat attcccgcca tagtaacggt tataacctgc tgtaatcatt
     3901 gaggtttgat cagcaaaaat atcccattcc gtcataaagc gcggggagat attgtggttt
     3961 gacagatagt tgtcatagtc ataacgcaca ccgggcatta acgacacatt ccgccagcta
     4021 atgtgatccg ccatatacag cgtgtagttg tcaattccca ggctgccttt acctttatgg
     4081 taaatggtat ggttagtttt ctttcctgca gcattaatca cataggattc agactggtta
     4141 tggcgttcag tccatgcatc ggaatagatg tattccgcgc cgaagtaggg ttgatgcgaa
     4201 acatcaccca cggcgaattt ttgccagtcc aggcgtgttt tgaaggtgta attatctaca
     4261 gcctgggaaa tgtgtcctaa tccgccacgg gtacaacgcc ctgtaatatc accatatgta
     4321 catgaaagtt cggtgtacca gatgtcatga tcgtgacggg tataatcact aatatgatcc
     4381 caaccaacgg tggtacgcag tttggcccat gcgagctgcg tatccatatc ccatgccaga
     4441 ccgtaggatt tattacccat ttcgcgatca gactgcggga aggtgctggt attataatca
     4501 cggctggagc cggtatattt taaggttaaa tcgtgggtaa agcggtcgct ggcaaaccag
     4561 gtaaatttgc tcaatgcagt atcgataacg tttttatact gtgcccgacc ggcgacaatg
     4621 ccgtcattcg aaacataatc cgcgcgggtg atatcagact ggcggcgcga taaaccggcg
     4681 gtaacgccga agttatcagc gagttcctga ttaaacgaca aggtataaaa gttcttttta
     4741 aaatctgggg agtaataagt acttcctgaa gagccttgat taaatgcgct cttgttattc
     4801 tcatcgatat gcgatgttaa ccagtccgaa cgcgtagtgc gataacccaa tttcaccttg
     4861 ctatcatcag cgttgaagcg tttgatcttt gcatcaatta ccccgccatt gaagcgacca
     4921 aattcaaccg gcacaacact gtcataaagc gtcacattgt ccagtaagct gacatcaaga
     4981 taatacccct gtgacatccc gctaatattg gttgcactac tggcatcgga ctcattcgct
     5041 gggttcaagt tattagtggc actaataccg tcaatcaaat aggcattctg atagggcgac
     5101 gcaccgtgaa tagagatttt ctccgggcga atatctccct ggttcaacga ggtactttgc
     5161 gttgaatcca tgcgtaccgc agggttggtt ctcagcagat cgctgatatt gccattcccg
     5221 gtcggtaaac gctcgatgct ttcactggtg taatgcgtgt tgccgtttac gggtacaggg
     5281 acaggagcat agaccgtcat ctcttcaaaa acatgcatgt acatgttttt gtcatcgacg
     5341 gcctgcgcca catcagcgcc acataaaatg acgccaggaa taagaactcg cttcatttat
     5401 ttatatctcc gtacagcagg gcttttattg tttttataaa accgcgctaa tgtcacaaat
     5461 atcatctgcc aggttccaga caccggattg atgtgtaacc ataatgacgc cgcttgtggg
     5521 tagtttttca cgcactaaac gcagtaagcg gatagcctct tgttcctcaa gatgagaggt
     5581 agtttcgtca agaaatatcc atttcggacg tcgtaaaatt aatcgtgcca gggcgatacg
     5641 ttgtttttcg ccgctggaaa gaatatctcc ccagcgatcg tggtcatgaa tacgcgcagc
     5701 caatttcccc agaccaacct gatgcagtac ttcgctcaac gatttatcgt ctacgggcag
     5761 gggaagtgct ttacaaataa tctctttcag taagccggtt ttgattaacg gtgtttgtga
     5821 cacataccaa ctgtcagcag gagaagaaat atcaccttta aaccacggcc agcagtgaga
     5881 taatgtttta agcagtgtgg tttttcccgc accagagtaa cctttcagta atagccattt
     5941 gcctggcgaa acatgaaagt tcaggttctc taatatgatc ttattatcag gagtacgaat
     6001 actcgcgtca gccacttgta ccgcatgttg gcaattttta ggtttattcg tagggcgctg
     6061 ttcggtgagt tgatggaact catacaaacg atcgataacc gcagccagtt cagcaagttc
     6121 gtcatattta taaataaacc agcttaaatt gttcgatacc agcataaatg cctggcgcga
     6181 tttcatcagt ccgcccagat tgatctgccc gctaataaac tgcggcaata acagaaagta
     6241 gggaagaacg ctaagcgagc gcgaataaat attctgccag taatcaagcc accgctgacg
     6301 attcattaaa cgatgccagt tctctttaat ggtatgaaaa ttgtcgctca actcctggcg
     6361 ttgtagagac tcagcattgc ttagcgcaat cagttctgcc tgcttattat gctgcacaag
     6421 atttgttcga aatgtcgctt cgctacgttg tttttccaca ttaagcggac gaatacgttt
     6481 accaacctta tgggtaaata aagttccacc gatcacaatg agcacaacgg tatagaccat
     6541 atacccctgg atattccatt ctgttccacc aacagtgaat gagagcgtac ccgcgctttg
     6601 ccaaagaata acggtaaagg tgatcagcat gctaagtgac tggatgaagc caaatgaaag
     6661 gctgagcgtt ttgctgatca gtaagagaat gtcttcagcg atacgttggt cagggttatc
     6721 tgtattttta tgctcgccgt agatctgcgt gaagtagtag tttttatctg caaaccaccg
     6781 attaaggtag taatctgtta accattcgcg ccagcgaata gtcagtagtt taattaacca
     6841 tgttttgttt acggatatta atacaaagat ccccagcagg gcagggaacc aaaggacaag
     6901 ctgccaaagc ttatcggttt ctttctggct tagcgcattg aaaaaatcat tattccagtc
     6961 gtttaaccag acctgaattt taaccacgcc aaggatcatg gcgaggataa taatgattaa
     7021 caacactgaa gttttattat tcttacgcag ccaaaaggg
//
LOCUS       DAZGJW010000208         7035 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZGJW010000208 DAZGJW010000000
VERSION     DAZGJW010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.208"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..6236
                     /gene="efa1"
                     /locus_tag="ACHKIK_004612"
     CDS             <1..6236
                     /gene="efa1"
                     /locus_tag="ACHKIK_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HFO9085635.1"
                     /translation="IHNIDKVIFKGQTLDRLYHEGYVFSDINTLSRYTLHGLGITGVH
                     TEENLLPAPSSSLINILKEHYNEDEISAKLPLAYDYILNKKESSSIPVEILNKLSELP
                     PHELLTPVLGQSVNPLGMGYSSDNGKITEQVIVSGADGFDNPISGLIYTYLEDLYNIH
                     VRMREGTLNSQNLRQLLENSVSSCFLTEQSINKLLSEAEKRPYQSLTEIHQHLTGLPT
                     IADATLSLLSVGLPGTGKLLRREQDYGRPPVTAIQDSTFVLPYNFKGIGFNDNIISSA
                     PVVSSLHFIAEHAKYTLLSWPEFYRHHAQRWFEMAKGYGSQNIDFHPQSLLVTQEGRC
                     MGLALLYLQTEDTAHYSILQENLMTVSALHQTSNRDKLPLSKDDNSLMTRTYSLIEML
                     QYQGNKYITNESLLHKTAWNQERITLLFNEKGVKRALISTPNHTLVLQQLEDIYRLTD
                     PNFGHADFLSPIDALKFIEAMIQLTPTLQEYYGLLNKDINKHIQVHYAESDMVWNKLL
                     PENDAGLSTRIQHTTTDRLANLAEPVAVAGISLPVKTLYDIGATLDGRRITSPPTSEQ
                     IPSLRLNGDVLNDYLSRTVLTPEQADNIRKILHTQGIRSGTRPIDPEMIRGTQDDLVS
                     SQTRLQRQATRVKQQLAGVLDTLQQHFQNIPRSSGRHLSVENIELADIGSGRFNLQIR
                     DGETLHTTSVEVPEVVSRFQKLSTMLSALPASGIMDFDLGMSVVGVVQYARLLQQGHE
                     DSTLAKINLAMDIKQLSEATLGSMIQIAGNKFLNTEGIQGFRLESAVAEGMRSVATRT
                     GGTMGKALSASARVLELPVLETVLGTWNLYNSVIQLQQATSYSETMAARVQIAFDSIS
                     LGLTAASVAFPPLIIATGPIAAIGMGASSIARNVARKEERHTQWLEYKKFLTDGSKHI
                     VVASPERGLLDFSGNKVFGKMVLDLRQSPPLLHGESSFNADRKIGHRPDLGDWQIREK
                     VGYANSISPYSSLAHGYANSKWPQTIPKIPSGEYDTIILGYGHQYQANTEIEYLSNWI
                     VWREAVPDSTSRHKRPPLEVLNSQCTVIAGERKTTVLPLRVLSDLTPECTEQAISLKD
                     YKFILRGGSGGLAVQVGGAGYYDIDANPVAKENTLSFRGLPEEFPLAFDLSKQTQSVM
                     LKTTDGEVPVMTITQKGINTLVGTTAGENWLTGNDKDNTFHTSSGGGTVISGGGNNRY
                     IIPRDLKTPLTLTLSNSSVSHEILLPETTLTELKPVAFELSLIYWAGNNIKVQLEDET
                     QLNRFAGNFRGHTSDGITLEAVSQENGIQLGVSSCDVQRWQAVYPEENNRPDAILDRL
                     HDMGWSLAPEVRFRGGETSASYDPLNRQLVYRLQERYSEFRLTGSRHYTTAVTGTPGS
                     RYIITGPDTAQISPAQIILAGDNDHPETIDLLEASPVLVEGKKDKNSVILTIATIQYS
                     LQLTISGIDESLPGTTRVAIQPQDTRLLGDVLRILPDNGNWVGIFRSGHTPTVNRLEN
                     LMALNQVMTFLPRVSGSAEQVLCLENLGGVRKKVEGELLSGKLKGAWKAEGEPTVPVN
                     ISDLSIPPYSRLYLIFEGKNNVLLRSKVHAAPLKITSAGEMQLSERQWQQQEHIIVKP
                     DNEAPSLILSEFRRFTISSDKKFSLKLMCHQGMVRIDRRSLSVRLFYLREQPGIGSLR
                     LTFRDFFTEVMDTTDREILEKELRPILIGDTHRFINAAYKNHLNIQLGDGVLNLADIV
                     AEYARIQKEETSKILYQYQGAMKKKTDGPSVVEDAIMTTTVTTDSGEPFPTFHPWYTD
                     DLSGRYKSVPMARKADTLYHLTPKGDLQIIYQVATKMVNQAMIVSLPNYRHEWEKYNL
                     SILSEIPQNNNTVVHSILRVNGPTMQVRTIDYRGTDENNPIVSFSDTTFINGEQMLSY
                     DSHSSGRVYSREEYMMWELQQRVSEASSARTQDYWLMDAAVRNGEWKITPELLRHTPG
                     YIRSTVSKWSRGWLKTGTILQTPEDRNTDVYLTTIQNNVFSRQGGGYQVYYRIDGMAG
                     ADIADNAPGETRCTLRPGTCFEVTSVDERHYEWNIIYVTLKTCGWSRNGQSKTPNGDN
                     LFN"
     gene            6408..6758
                     /locus_tag="ACHKIK_004613"
                     /pseudo
     CDS             6408..6758
                     /locus_tag="ACHKIK_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2091 a         1596 c         1599 g         1749 t
ORIGIN      
        1 ccattcataa tatagataaa gttatattta aagggcaaac actggatcgt ctttatcatg
       61 aaggatatgt attttctgat atcaatacct tgtctcgtta tacactacac ggactaggaa
      121 taactggtgt acatactgaa gaaaacctgc taccagctcc ttcatcgtcc ttaattaata
      181 tattgaaaga acattataat gaagatgaaa ttagtgcgaa attaccacta gcatatgatt
      241 acattttaaa taaaaaagaa tcaagctcta ttcctgttga aattttgaac aaactttcag
      301 agttaccacc acatgaacta ctcacacctg ttcttggcca gagtgttaat cctctgggca
      361 tgggctactc atccgataat ggaaaaatca cagagcaagt aatagtcagt ggagctgatg
      421 gatttgataa tcccatatct ggacttatat atacctatct tgaagatcta tataacatcc
      481 atgtaaggat gcgagaaggt acactaaatt cacagaatct tcgtcagctt ctggaaaact
      541 ctgtttcttc atgctttttg actgagcaaa gtattaataa attacttagt gaggcagaaa
      601 aaaggcctta tcagtcttta acagaaatac atcagcatct cacaggatta ccaactattg
      661 ccgatgcaac cctttcatta ctttctgttg gattacctgg tacgggaaaa ctattgcgca
      721 gggagcagga ctatgggcgt ccaccagtta cagcaattca ggattccaca tttgtactcc
      781 cttataattt caaaggtatt ggttttaacg ataacattat atcctctgca cctgtagtct
      841 cctcattaca ttttatcgct gaacatgcga aatatacttt gttgtcatgg cctgagtttt
      901 atcgtcatca tgcacagcga tggttcgaaa tggctaaagg atatggaagc cagaatattg
      961 attttcaccc tcagtctcta ttggtaaccc aagaaggacg ctgtatggga ttagccttac
     1021 tttatttaca gactgaagat actgctcatt atagcattct ccaggaaaac ctaatgactg
     1081 tgagtgcact tcatcagacc agtaatcgcg ataagttgcc actgtccaaa gatgataatt
     1141 ccttaatgac aagaacttat agtctgattg aaatgctaca gtatcaggga aacaaatata
     1201 ttaccaacga atcgctacta cataagaccg catggaacca agaaagaata actttattat
     1261 tcaatgaaaa aggagttaag cgagccctga taagcacgcc taatcatact ctggttctgc
     1321 aacaactgga ggatatttac cggctcactg atccaaattt tgggcatgca gatttccttt
     1381 cacctataga tgctctgaag tttattgagg ctatgataca attaactcca acacttcagg
     1441 aatattatgg cctattaaac aaagacatta ataaacatat acaagtacat tatgccgaat
     1501 cagatatggt ctggaataag cttctgccag aaaatgatgc tggactgagc accagaattc
     1561 agcacaccac caccgaccgt ctggcgaatc tggctgaacc agtcgctgtt gcaggtatct
     1621 ccctgccagt aaaaacactt tatgatatcg gagccaccct tgacggtcgg cgcatcacct
     1681 ctcctccaac atcggagcaa atcccttctc tgcgtctcaa cggtgatgtt ctgaatgatt
     1741 atctgtcccg cacagttctg actccagaac aggctgataa cataagaaaa atactgcaca
     1801 ctcagggaat acgcagcggt acccgtccca tagatccgga gatgattcgt gggacgcagg
     1861 atgacctagt ttcgtcacag actcgtctgc aaaggcaagc aacacgggtt aaacagcaac
     1921 tcgccggcgt acttgatact ctgcaacagc acttccagaa cattccacgt tcatccggtc
     1981 gtcatctttc tgtagagaat attgagctgg ctgatatcgg aagtgggcgt ttcaaccttc
     2041 aaattcgaga tggagaaaca ttgcatacca cttctgtgga agtaccggaa gttgtgtccc
     2101 gttttcagaa actttctacc atgctttcag ccctgcctgc cagtggaatc atggatttcg
     2161 acctcggcat gagtgtggtc ggagtcgtcc agtatgcccg cctgctacag caggggcacg
     2221 aagacagtac cctagccaag ataaatctag ctatggatat caagcaactt tccgaagcaa
     2281 ctctcggcag catgattcag attgccggga ataagtttct caatacagaa ggaatccagg
     2341 ggttcagact ggaaagcgcc gtcgctgaag gtatgcgctc agtagcaacc cgtaccggag
     2401 gcacaatggg gaaagccctt tctgccagtg cccgtgttct tgaactgcct gtactggaaa
     2461 cagttctggg gacatggaac ctgtacaaca gcgtcattca gctccagcaa gctacatctt
     2521 attctgagac aatggctgcc cgggtacaga ttgcgtttga ttccatttct ctgggattaa
     2581 ctgccgcttc ggtagctttc ccgccactaa ttattgccac tggccccatt gcggctattg
     2641 gtatgggagc ttccagtatt gcacgtaatg tggcacggaa agaagaacgg catacacaat
     2701 ggctggaata taaaaaattc ctgactgatg gcagtaaaca cattgttgtg gcctctccgg
     2761 aaagaggtct gctggatttc tccggaaaca aagtttttgg aaaaatggtg ctggatctgc
     2821 gtcagtctcc tcctctcttg catggagaaa gctcttttaa cgctgaccgc aaaatcggtc
     2881 atcgtccgga tctgggggac tggcaaattc gtgagaaggt ggggtatgcc aacagtatca
     2941 gtccctactc ttccctggcg cacggttacg ccaacagtaa atggccacaa acaataccga
     3001 aaattccctc gggagaatat gacaccataa ttctgggcta cggtcaccag tatcaggcca
     3061 atacggaaat agaatatctg tctaactgga ttgtatggcg ggaagccgta ccagacagta
     3121 cttcccgcca caaacgtcct cctctggagg ttcttaatag tcagtgtact gtgatagctg
     3181 gagagcgtaa aaccacagta cttcccctga gagtgctcag cgatctgaca ccggaatgca
     3241 cagaacaggc tatatcgtta aaagattata aattcatact gagaggggga agcggtgggc
     3301 tggctgttca ggtcggtggc gcgggatatt atgatattga tgcaaatcct gtggcaaaag
     3361 aaaatacgct ctcttttcgc gggctaccgg aagagtttcc gctcgccttt gatttatcaa
     3421 aacaaacaca gtcggtcatg ctgaaaacaa cggacggtga ggtgccggta atgaccatca
     3481 cccagaaggg aataaacacc ctggtcggta caacggccgg cgaaaactgg ctaacgggta
     3541 acgataagga caataccttc catacgagtt ctggtggcgg tactgtcatc tctggtggag
     3601 ggaataaccg ctatattatc ccccgcgatt taaaaactcc gctgacactg acgctgtcta
     3661 atagctcggt ctctcacgag atccttctgc cagaaacaac tctgacagaa ttaaaacctg
     3721 tcgcctttga gctgagtctg atttactggg ccgggaacaa tataaaggtt cagctggagg
     3781 atgagacaca actgaaccgt tttgccggaa acttcagggg gcataccagt gatggcatta
     3841 ctctggaggc ggtttcccag gaaaatggta ttcaactggg ggtttcatca tgtgatgttc
     3901 aacgctggca ggctgtttat ccggaagaaa acaacagacc agacgccata ctggacaggc
     3961 tgcatgatat gggatggagc ctggcccccg aggtcaggtt ccggggagga gaaacatctg
     4021 ccagctatga ccccctgaac cgtcagctcg tttaccggct tcaggagcgt tactcggaat
     4081 tcaggctgac cggcagtcgc cattacacca cggctgtaac cggaaccccc ggaagccggt
     4141 acattattac ggggccggat acagcacaga tatcaccggc acaaatcata ctggccggtg
     4201 ataatgacca tccggaaacg attgatttgc ttgaggccag tcctgttctg gttgaaggga
     4261 aaaaagacaa aaacagcgtg atattaacga ttgctacgat tcagtattcc cttcaactga
     4321 caatatccgg gatcgacgaa tcgctgcccg ggacaacccg tgtggcaatt cagcctcagg
     4381 atacccgttt actgggtgac gtactccgga tcttaccaga taatggtaac tgggtgggga
     4441 ttttccggag tggtcataca ccaacggtaa accggctgga aaatttgatg gcactgaatc
     4501 aggtaatgac gttcctgccc cgggtatccg gaagtgcaga gcaggtatta tgcctcgaaa
     4561 acctaggtgg cgtaaggaaa aaagtggagg gggagttact gtcagggaag ctgaaaggtg
     4621 cgtggaaagc cgaaggtgaa cctactgttc cggtaaatat ctcagatcta agtatcccac
     4681 cctattcacg tctgtatctg atttttgaag ggaaaaataa tgtgttgcta cgcagtaaag
     4741 tacatgcagc tccgttgaaa ataacatccg caggagagat gcagttatct gaaaggcagt
     4801 ggcaacagca ggaacatatt attgtcaagc ccgacaacga agccccctca ttaatactca
     4861 gtgaatttcg tcgtttcact atttcatcgg ataaaaaatt ttctttaaaa ctgatgtgcc
     4921 atcaggggat ggtccgcatc gaccgcagat cattatcggt cagattgttc tatctgcgtg
     4981 aacagccagg tatcggcagt ttacgtctga cgttcagaga ttttttcaca gaagtgatgg
     5041 atacaacgga cagggaaatt ctggagaaag agctgagacc aattctgata ggagatacac
     5101 accgctttat caacgctgca tacaaaaatc atctgaatat ccagttagga gatggcgttc
     5161 tgaatctggc agacattgtt gcggaatacg cccgtattca aaaggaagaa acatcaaaaa
     5221 tattgtatca atatcaaggt gccatgaaaa aaaaaacaga tggaccatct gtggtagaag
     5281 atgccattat gaccactact gtcacaacag attcaggtga accattccct accttccacc
     5341 cgtggtatac agatgattta tcagggcgtt ataagagcgt acctatggca agaaaagcag
     5401 atactttgta tcacctgaca ccaaaaggtg atctacagat aatatatcag gtagctacaa
     5461 aaatggtgaa tcaggcgatg attgtatccc tgccaaacta ccgacacgag tgggaaaaat
     5521 ataatttaag catcttatcc gaaatccctc agaacaataa tactgttgta cattcaatcc
     5581 tcagggttaa tggccccaca atgcaggtgc gcacaattga ctacagagga acggatgaaa
     5641 acaatcccat agtatctttt tcagatacaa ccttcatcaa tggtgaacag atgttgagtt
     5701 atgactcgca ttcatcaggg cgagtctatt ccagagaaga atatatgatg tgggaattgc
     5761 agcaacgggt atcagaagct tccagtgccc ggacacagga ttactggctg atggatgcag
     5821 cggtaagaaa cggagaatgg aagatcacac cagaattatt acgtcacaca ccgggatata
     5881 tccggagtac ggtatcgaaa tggtccagag gatggctgaa aaccggcaca atactccaga
     5941 ctccagaaga cagaaatacg gatgtatacc tgactaccat acagaacaat gtatttagtc
     6001 gtcagggggg cggctaccaa gtgtattatc ggattgatgg aatggctggt gcggatatag
     6061 cggataatgc accaggggaa acccgctgca ccctcaggcc cggaacatgt tttgaagtga
     6121 caagtgtgga tgaaaggcat tatgagtgga atatcattta tgtcacgctg aaaacctgtg
     6181 gctggagccg aaatggccaa agcaaaacgc cgaatggtga caaccttttt aactaattaa
     6241 aatctctgtt aaagatgtat aacccggcat ctatgtgctc accagcatat agatgcaatg
     6301 tgattcccct gatggtcgta tcagagatgg tatgcgagcc tgtacataga tttgtgtaat
     6361 tgcctgattt tgatatgttc aatccaacat caaaagcagg ttaatttatg gacgaaaaac
     6421 agttgcaggc tctggctaac gaacaggcca aaaatctcaa aacccctgaa gatctcagcc
     6481 agttcgatcg cctgctgaag aaaatcagcg ttgaggcagc tctcaacgct gaaatgtccc
     6541 accatctggg ctacgataaa aaccagccta aaccgggtgc caactcccgc aatggctatt
     6601 ccacaaagac cgttatcacc ggtgatggcc ccctggaact acgcactccg cgcgatcgtg
     6661 atggctcttt tgaaccgtaa cttgtgaaga aaaaccagac ccggatcacc gggatggata
     6721 accagatcct gtcgttgtac gccaaaggcc tgacaaccgt aagcgtacag cctgaaccgt
     6781 ctggtcagaa tctgacgaat tagagtaagc tggacatccc cagatatatt atggctgcca
     6841 gcgtcatatc ggcataaata gagatatccc ttaacgctaa ccagaaaaaa atacctataa
     6901 gagcctgtac atagagttgt gtcgttgcct gatgttgata tgtcaaatcc aacatcaaat
     6961 agcggtaaat ttatggataa aaaattacag gctctggtta acgaaacacc cccctcttgc
     7021 tctattcccc tcttt
//
LOCUS       DAZGJW010000209         7010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZGJW010000209 DAZGJW010000000
VERSION     DAZGJW010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.209"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(88..221)
                     /locus_tag="ACHKIK_004614"
     ncRNA           complement(88..221)
                     /ncRNA_class="other"
                     /locus_tag="ACHKIK_004614"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(256..332)
                     /locus_tag="ACHKIK_004615"
     tRNA            complement(256..332)
                     /locus_tag="ACHKIK_004615"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(296..298),aa:Pro,seq:cgg)
     gene            complement(424..2115)
                     /gene="eptB"
                     /locus_tag="ACHKIK_004616"
     CDS             complement(424..2115)
                     /gene="eptB"
                     /locus_tag="ACHKIK_004616"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO9085636.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            complement(2368..2892)
                     /gene="lpfE"
                     /locus_tag="ACHKIK_004617"
     CDS             complement(2368..2892)
                     /gene="lpfE"
                     /locus_tag="ACHKIK_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO9085637.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            complement(2898..3953)
                     /gene="lpfD"
                     /locus_tag="ACHKIK_004618"
     CDS             complement(2898..3953)
                     /gene="lpfD"
                     /locus_tag="ACHKIK_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFO9085638.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            complement(3964..6495)
                     /gene="lpfC"
                     /locus_tag="ACHKIK_004619"
     CDS             complement(3964..6495)
                     /gene="lpfC"
                     /locus_tag="ACHKIK_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO9085639.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            complement(6519..>7010)
                     /locus_tag="ACHKIK_004620"
     CDS             complement(6519..>7010)
                     /locus_tag="ACHKIK_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO9085640.1"
                     /translation="KTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESVYWINVKA
                     IPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQIVVNNDSA
                     YNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGVAGEMLTK
                     PIN"
BASE COUNT         1782 a         1800 c         1627 g         1801 t
ORIGIN      
        1 gagtcgaacc tccccgcccc cggaggttct catcctcatg agctacaaat gcaaaaaagc
       61 ctgctcgttg agcaggcttt tcgaatttgg tcggtgatag aggattactc gccacttcgt
      121 ggctcgccct gcgggccgtt gctggcgcaa cgttctctcg cttcgctcga gtcgaacctc
      181 cccgcccccg gaggttctca tcctcatggg cttcaaatgc aaaaaagcct gctcgctgag
      241 caggcttttc gaatttggtc ggtgatagag gattcgaacc tccgacccct tcgtcccgaa
      301 cgaagtgcgc taccaggctg cgccaatcac cgaatgcggg gcgcatctta ctgcgcagat
      361 acgccctcgt caatccctta atagcaaaat gccttttgat cggcgagaaa gtcagcaggc
      421 cgcttagtta gccgctgcca cttttgtctg cgggatgtga caccagttgt tgttttcgtt
      481 aattccacca tccggtgaag tatagccaag acaacccatg atggtgtcgt acagctctac
      541 atgacggcgt ggcactttca tgtcggcttc ttttttcagc tgcgcaaatg cctgagcatt
      601 ggccggattt tccagatatt tatctgacat ccagaccatc atcggtacgc ggaactgctc
      661 cggcggtgcc agttcacgcg gcgtgccgtg caggtgctcg cgttcattaa ttgactcacc
      721 gtggtcagct gcgtagaaca caatcgcttt cttatcgcga acctgatcaa tcacgctgga
      781 gataaagtga tccacatagg tcaccgagtt gtcataggag ttgatcatct gcgctttggt
      841 acagccgcta tcaacaccaa tacattccgg cttccactgc gcgaagctac gcggataacg
      901 ctgggtgtag ttaaaatgcg aacctttggt atgcagaatg atcagatgct taccatccgg
      961 gttgcgccct agcgattgct gcatttcgtc taccagcaac atatcatcta ccggcttgcc
     1021 acgattacgt ggctccgcac caatctgctc acgataagca atgttgtccg ccatcgtgtt
     1081 gctgtagaac cacatttcgc tctgcatagc gtagaggtca gaactgaatc ctaactgctt
     1141 cagaaccgcg aaaatgttct gttcttttaa tgtgcgctgc ggattatctt ccgcgccccc
     1201 ctgacgtaca aacatgcaac gcagtgagag tttggttgcg gtatcacagg agtaaccacg
     1261 gaacgccgcc agatttttct cctgggccag tttcggcgtg gtattacgct catagccgaa
     1321 aatgcccata tggtcccaac gcgtggtttc accgatgata aacacgacat aagtgtcatc
     1381 aacgttttgc ggtgcctgat aagtgaattt cttcgccgga ttaagcaatg aattattatc
     1441 ggaagattca tcgacccgcg cccaggcata cagccccaac gcagaaagcc agtttgatgg
     1501 cagataagag ttcgctacga caccgccata actcggcaaa tcaacgccgg tcgccctctc
     1561 cactttcttc tgctggatat ccagcaaacg aatcggtgcc caaaccataa tacccgccag
     1621 tacgacgacc gccaggctgc gaatacgctg ccctggggtt cgcagttgtc ggagcaaggt
     1681 gtagcgacag cggttattcc agataaggat aagaggcaat gcactaacgg cgattaacca
     1741 gagaataaag ttcagaccaa caacttcttt tgacaggtcg atatcggtgg tcatgacgga
     1801 agcgatgatg ccataaccaa tgaccacatt aaggaaggtc atgtaatagc tggcacctgc
     1861 ggaaaagagc accaccagcg acgccagaat acgccagctg cggcggccaa acagcgatag
     1921 aagacgtagt aaaaagaagg tcaccagtac ggtggcggcc agttcaacaa cagcagaaac
     1981 gcctttccag acggtaaaat cgtgcgcata gctgccgaag cggcgataaa aaaccgcgcc
     2041 attcataaaa aggccaatat agattgcaag caaaaagctc agcttctgct gtgtaatcga
     2101 tttgatgtat ctcatgcaaa caaaccctgg acaaacaggt gataacaccc tgcttagcgg
     2161 cttttcaggc ccggcagggg aaaagtgcgg aggtagtcag ataagtaaaa tctacacgcg
     2221 cgaataagta gaccacagca aagcgaaaaa gtgtggggaa agagtgtgca tgaagcacaa
     2281 atttacaaaa atgcaagttt gaaaagagac tttagcgtta caaaaagcag taaaaagggc
     2341 cagttaactg gcccttaaga tgtgaaatta ttcgtactgt acagtaaagt cgaccgttgc
     2401 atctgcgcga ccaatagttg catcggcgcg aataggcacg tagctggcga tatagttcag
     2461 ttgatgtgcg ccatcaccgt taattgcaaa ctcaaccggt gtcggcgttg tattttccgg
     2521 gaactgttgt actccagcag cggttttgat tttcagccca acgcctgtag cgccacccgc
     2581 atccagcgca aaaagatttg agtcgtctgc attgggtgta ccgtccatag tcacgtaggc
     2641 tctgaccact tcgctacagt ttttcaggcc gatggaaaaa ggagtgggat taatttccgt
     2701 tccgattctt accggctttt tgagaaccgc ggtgcccatg tcaacctcaa cgctgtcttt
     2761 caccagttcg caagcaccct ggctgatttt aagggtgaat ttcaatgttc ctttcggccc
     2821 taaatccgtg gttgccgcga atgacgaaaa ggaaagaggc agcaggcata acgcaagtat
     2881 tgttcgcttc atgactgtta ctccagttcg acatgtatgg tagcaatacc gctataatcc
     2941 ccggcagcgg gtaattttcc ggtagtgcta acgggataag cggtaattgt ggttgtcgct
     3001 ttgcgtttga catcttcata cagcgagcct gggttcattt ctaataagct attactgtca
     3061 ttcggcttaa gaatattacc tttaccgtcc tcaatgactg caccaacatc ggcgttgcct
     3121 aaatcaattg cgctgggata ggcagtattt ggtgttgcat caatactgag ataaattttg
     3181 acgccatctg aaatattatt gcagtcaaag cttaattctt tttgtacctt ctgaacattt
     3241 tgtggtggtt gtccggcacg ccctttaaaa tcatgggcct gatattctcc gaatggaata
     3301 tccagtacgg tgccagcggc aagttcacag ccttgagtaa ctgtaatact cccggaaata
     3361 ctgacattgg ccatgggctg actatcattg taaacacctt tcttttttgt cccaaagagg
     3421 gacgcaactc gtgttttagg aataatcatt tgcccaacaa aaggatgtga aatataaata
     3481 ctgatacttc ctttaccacc tgtcccccaa ttgttagcag tatagggcgt acattctttc
     3541 tgtgccgtgc cattgcttcg atcggtaaaa ggcaccggga tatctaattg tgtgtagaca
     3601 tatattttgg tgagaatatc aatgtaatta ttgaatttat agtacccatc tacagttcca
     3661 ggctcaggca gaagcgttac acctttaaaa taagtcacaa ctccattatc tggaatggga
     3721 tccgggcatt cacaataagc ggaatatttt gtacctaaag caaagggctg agttaatatt
     3781 ttcccagcct tattttctga aggtacctgg atagtctgta cgaaatcaaa actatactca
     3841 tgggccaccc catttgatgt ctgacatggt ccccaatccg cagcccatat atttccagca
     3901 aaagtgctcg tccctaacag caccatacct gccaacaaat ttttaatttt catctaaata
     3961 tccttatttc tcgcataccg cattaaccat cacgatagcc gcattgtcct tgttctctgg
     4021 caagcggaat ggcacacggc aacgttgcgc ctgctcgttc ccccagctca cctgcaattc
     4081 gccctcttct ggcatcccgc tgatataaag ctgtccttct tcacccacaa tgctgctggc
     4141 aggtttgctc tcatcaatta acgctgcggt tgcaccaaac ggtactgcgt tgccattcgc
     4201 ctgttttaag ctcatcagca cacggtaacc gacgcgggta tcaaagcggg aacgcacaac
     4261 tgcaccacga gtaggcacga ctttctggaa cgcttcctgt agttcaacgt tttgccccaa
     4321 cgtgtcgcta cgcaattcca caatggtttc acgatatggt gtgagataag gcaccacggc
     4381 gttgcccatc cagtcaactt ctacgccgct gctgttaacc acatgtccac cacgcgcgcc
     4441 cggtgcagaa acaatcgcca gactttcgcc taaaggctgc gaaagcgtta taccttcact
     4501 gtgaacgatg acaccgccgc gcacgccata gttaacctgt tggttatctt tagaatagtt
     4561 atagcctacg tcaatgttac cagagccacc catatacccc atgttgacgc tgccactgta
     4621 gcctacgcca ttactggtgt aaccctgctg aacgttatag ctcaggttac gatcttccag
     4681 taacgtgccg ctaacagagg cctgttgcga cgtcttgcca ttctggtcgg tagtcatgcg
     4741 ataactgctc cacgcgcccc ccagagggat ggaaacactg aatgaccaca agcgatcgtc
     4801 ttcttcccat tccggacttc tcgtccaggt ataggccacg ctgtaattca cccgaccaat
     4861 gtgaccgtga taaccggcgc ttaaggagtt ttccttaccg tcaacgttcc agtaatcctg
     4921 ctgcgtcata ttgaaataaa ccgagccgta gtctcccagt tgctgggtaa tattgccctg
     4981 tatctggctg cgcttgtgat aacggcggta atcactgtcg gcgtcactgc gaacatcagt
     5041 tgcttcctgg aaggtgtagt agccgctggt tgagtatttg tagcccaaca gacgcaggtc
     5101 agtgccaccc tcaaaacttt tcgaatagag gaagcgataa gactggcctt cctctttttc
     5161 attgttgcgt aactgacttt tcgcctgagt gacatccacc gatactgcgc caatggtacc
     5221 gaagtttttc cccaggccca gagctaaagc gttgtaatct gccgacaaca atgcaccacc
     5281 gtaggcggtc ataccccacg gcaagccgta catggcggta aactgcccga aatgtggttt
     5341 atcgctgtcg taattaccag cacgatattc accggcagcc agactgtatt tcagataccc
     5401 ttcacgctgg aaaatcgcca ccgccgaaaa cggctggata aaacgttgtt cacttccgtc
     5461 ggcttcttta atgatcaccg tcaggtcgcc gctgccggag gttgcgtaga gatcgttaat
     5521 ctcaaatgca cccggctgaa caaaagtgcg ataaatgaca tagccgttct gttcgatact
     5581 cacttcagcg ttgcttttgg cgatgccgcg cactaccgga gcaaagccac gctggctgtc
     5641 cggcaacatt tcatcgtcgg atgccagtaa cgccccacgc atttgtacgc tatcaaaaat
     5701 ctcgccaggc gtggcggtat cacccagagt tatctgtgat ttaagtggaa taattgcccg
     5761 gtttaacgat gtaccaacgt tatcccattg gtttttgcca tcggtacggt tccagatact
     5821 gttattacgt aaacgccacg gaccaacgtt caaaccgctg cgcaggttca ggtaataact
     5881 gtcactggtc gaatctgaag atgaacctgt accttcattg cgtccattgc tgccactaaa
     5941 gctgtaatcc agcaacagcg caggtattcc ttcgtcccag cgagaaggat caaccgtgcc
     6001 gcgagcggtc atcatcattg ccgcctgtgg aaaggaaaga tgcagcgtct gagtattaaa
     6061 atcaaagcgg ctggtggcct ggggaatgat ttcatcaaac gctacgcagg cttctggtgg
     6121 tgacatcttc agcgccggaa agctgtcaac gcgcacgccc atttcttcca gttgcacccg
     6181 gctaaggcaa ggcataagcg actcaccgct ggctttgcgt ttcgcttcgc tggcggtact
     6241 aaaatccagc gtccgggaag ccattttttc ttcgtttaca aacaccgaaa cccggtattt
     6301 accggggatt tgctggccga caggtgagtc gtataccgac aagtcgatgt gttgatcgat
     6361 accctgaacg ttttcgagaa atttagggtt aaattttccg ccatacgcag gtaacgagct
     6421 ggcaatcgtt aatgccagca gagagaagga aaaatgcgct ctgtagtgat agagaggtaa
     6481 atgcatccaa tgcatagtaa aagcgccttg taaaaggatc agttaatcgg cttcgtcagc
     6541 atttcgccag caacaccgaa atcattaata atgctgtatt tcacttcgtg cgcgttgcct
     6601 gtaccggctg gaaggttaat gtttaacttt cctttcgcga gaaccattcc cgcccgttca
     6661 atcttttgtc cattaatgga aaactgatta aaggtcaggt tataagcgga gtcgttatta
     6721 accacaattt ggttattttg acgctggaaa cgtaactgtt tcatggcctc gaggttgctt
     6781 cccggcaaac cttttggacg atagaacaac ttcaaacggg tgcgaacggc tatctgtaag
     6841 acgtttttat tttcattttc agcacttttc gcaggaatgg cctttacatt aatccagtaa
     6901 acggattcac gatctttagg taaggtatct tcaatattga cgatacgtaa gacgttattt
     6961 ttatcaggat caagtttgaa taatggcggt gtaataataa acggtgtttt
//
LOCUS       DAZGJW010000210         6993 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZGJW010000210 DAZGJW010000000
VERSION     DAZGJW010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.210"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(276..761)
                     /gene="sixA"
                     /locus_tag="ACHKIK_004621"
     CDS             complement(276..761)
                     /gene="sixA"
                     /locus_tag="ACHKIK_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO9085641.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(964..3108)
                     /gene="fadJ"
                     /locus_tag="ACHKIK_004622"
     CDS             complement(964..3108)
                     /gene="fadJ"
                     /locus_tag="ACHKIK_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO9085642.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3108..4418)
                     /gene="fadI"
                     /locus_tag="ACHKIK_004623"
     CDS             complement(3108..4418)
                     /gene="fadI"
                     /locus_tag="ACHKIK_004623"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO9085643.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4598..4882)
                     /locus_tag="ACHKIK_004624"
     CDS             complement(4598..4882)
                     /locus_tag="ACHKIK_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO9085644.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFASRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5254..6594
                     /gene="fadL"
                     /locus_tag="ACHKIK_004625"
     CDS             5254..6594
                     /gene="fadL"
                     /locus_tag="ACHKIK_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO9085645.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1759 a         1861 c         1639 g         1734 t
ORIGIN      
        1 gtttcaatta catcctgcct gattattcaa ctctattttc tctattatct gtattaataa
       61 atgacaatga gtgtcaaaca gacattattc atttcaacat gtaactttgg gcaaaatcaa
      121 gatacgcgca gaagaataat ttccgaatga aagtagaaag tgttttattt atagatgggg
      181 agcgtcttcg atagtagtga ggttgacaga tatccgctgc ctgatgagca attatgccca
      241 atgaatctac ctcattgggc ataatttgga actcatcaga tagcttttgc catcttcaga
      301 ttacacgggc tcatctgcca gttgaatgtg ccgtttccgc tttcatcaag cgttacgctg
      361 gcaatggccg aggtggtaaa cataggcggc gtttcgcccg gacataactc ggcaaccaga
      421 tacccgacta acggtaagtg agagatcacc agtaccgaag caacaccttc attggtcaac
      481 gcctgcaaat aggcactgac cagaccaaca tcgccgcagg gcgttaactc cggcagaact
      541 tctgcactgg aaggcaggtt cagacaatcc cctacttctt ccagtgtttg ctcggctcgc
      601 aggaacgggc tcaccagaac gcgttcgatt tccacttttt gacctttcag ccagttcgcc
      661 atcaggcgag attcgtcaca accattagtg gtcagaggac gaacggaatc actggcggca
      721 tcgagggctg cgtcgccgtg acgcatgata aaaacttgca tattgcaccg cttttgttaa
      781 ccagattcgc ccgttttctt tcatctgaaa ccagagaaag aaaacggtgg ccggcattgt
      841 gccttatcca ttcaccgaat gaaacgctgt tttttacctc aatggcgtaa gtatagtcaa
      901 tatgcgttta tattttgacc aacactccgc cattcagcgc ggattcatat agctttgacc
      961 ttcttattgc aggtcagttg cagttgtttt ccaaaaactt tccccacgcg cgcccatctc
     1021 gaccaaacgc tcgcaagggg taaaacggga accatactgc gtggcaagtc gttgcattat
     1081 tgcaaccact tcgcccgcgc cgagagaatc gatatagcgg aacggtccac cgagaaatgg
     1141 cggaaaacca atgccaaata ccgcaccaat atccccgtca cgcacgctac ggataacctg
     1201 ctcatcaaca caacgtactg cttcattcag catcaacatc acacaccgtt cagcaacctg
     1261 cggtgcggag agtcgcccct gcccttgtgc gccaatgagc gggtaaatgg cgggatcgac
     1321 ctgttttttg cttttacgcc ctttctgacc ataaagatag aaaccccgac cattttttct
     1381 gcctttgcga tcgtcgttca aaattgaaga aacaacattt gcaggcgcgc taaaacgttc
     1441 tccataagcg gcttccagta caggaataat tttagtcccg gtgtcgattc ctacctcatc
     1501 caaaagttgg attggaccta ccggaaaacc aaatttcact agcgcggcat caatgtgctc
     1561 tacccgttca ccttcggtca gcatgcggat agcttcatta atgtaaggcg ctaagatgcg
     1621 attgacgtaa aacccggctt tgtcacgcac gacaatgggc gttttgccct gttttttcgc
     1681 cagttttacc gtagtggcga tggtttgcgc cgatgtaccc gcatgaggaa taatctctac
     1741 cagcggcatt ttttccaccg gactgaagaa atgcaggcca ataacttgct caggtcgcgt
     1801 ggcgtgagct gcgatatcac caatcggtaa agatgacgta ttcgaagcaa aaatggtatg
     1861 agcggcgcaa ttttgctcca cttccgccac catctgttgt tttaattcga gattttcaaa
     1921 caccgcttca ataatcagat cgcgatgggc aaagccgcga tagtccgtcg ttccggagat
     1981 taatgccagc tgtttgtcac gttcgctggc tttgagatga cgacggcgaa ctttgccctc
     2041 cagctgatcc caactgtact tcagcgcatg atttatgccc tgcgggttga tatctttaat
     2101 tctgaccgga agccccgctt tacaagcagt gacataagca ataccgccgc ccatcaagcc
     2161 accacctaaa attcccacgc tgtttaatgg cgcaggcggt gcatcactac cgggatcttt
     2221 cttcacttcc gtactggcaa aaaagatact acgcaaagct tgcgattgtg gcgtcatcgc
     2281 cagttcgcca aacgcccgag cttcggcgtc ataaccgctg ctggtgccct gcgctaatcc
     2341 cgtttcaaca acctccagga tgcgttctgt cgccggataa ttgccttgag ttttgtgttc
     2401 tgttttcttg ccgaccattt tgaacagcag cgcacgacct aacggccccg ccagaatacg
     2461 ctcgcgtaca ggtagagggc gggaagatgg gcgatccttc ttcgccagct caacagcgac
     2521 ttccagcaaa atggagtgcg gaacaacgtc atccaccagc cccagcttta atgcctgttt
     2581 cgcccgaagt tgttttccgg tgaggatcat ctctaatgct gtgctgacgc ctatcagacg
     2641 cggtaaacgc tgggtgccgc ctgaaccggg taacaatcca agttgtactt caggcaaacc
     2701 gagcaccgtt ttaggatcgt cagtacaaac gcgaccgtgg cacgccagcg ccaactccag
     2761 cccaccaccc aggcaagcgc catgaatagc cgcgataacc ggaatgggca aagcatgaat
     2821 ctccgccatc aactgttggc cctgccgcgc cagtgcttcc gcttcttgcg ccgttttgca
     2881 gttgccgatc atgttgatgt ctgcgccagc aatgaagttg tccggtttag cggagacaaa
     2941 caccacgcct cgcaactctt tgttttcacg gagttgctta ataatggcgc gcacctgcga
     3001 ggcaaactcc gccttcaggg tattcatttt ctcacccggt acgtcgatgg tgataacggc
     3061 aatgttgtcc agacgaacat taagggtaaa cgctgatgtc atttccatta ttccgcctcc
     3121 agaaccattg ccgcgccaag cccaccggca gcacaggcgg taactaaacc aaatccaccg
     3181 ccgcgacggc gaagttcatg caatgtctgg gtaatcatcc gtgcgccggt cgccgcgaag
     3241 ggatgtccgt aagcaatcga accgccaagc acgttaaatt tgctatcgtc cacttcgcca
     3301 gtggcatgtg cacgccccag tacttcacga gcaaaacgtt cactacccag caactgaata
     3361 ttcgccagtg tctgagctgc aaaggcttcg tgcatatcga tcaatgtcag atcgctcatt
     3421 gtcaaaccgg cacgttccag cgccagcggt gttgaccagg ccggaccgag caacatgtcc
     3481 tgccagacat caatcgcagt aaatgcgtag ctgcgcagat accccagcgg caccagccct
     3541 aattctttcg cccgggattc agtcatcagg atcaccgctg ccgcgccatc ggtcagcggc
     3601 gtactgtttg ccgccgttac cgttccgtgt ttgcgatcaa acgccgggcg cagctttgcg
     3661 taatcggcaa gcgaggaatt accgcgaata ttgttgtctt cgacaagcgg ttgtttataa
     3721 ggagggataa aggcagtcat cacctcttct ttgagttttc cgtctgacca tgcctgagcg
     3781 gcacgctgat gcgaacggtg cgctaatgca tcttgctgtt ctcgggtgat gccgtaggtt
     3841 ttcgccattt gttcggcggt gtcgcccatc cgcaagccag tagaatattc tgctaccgca
     3901 ggcggtacgg gcattaagtc gcgcaaacgc aggcgagaga agagtttcag tcgctggctc
     3961 atggttcgag ctttgttgac atcaaccagc acgcgcgcca gttttttact gacgccaatt
     4021 ggcaatacgg aagaggaatc tgccccaccg gcaatccccg ctcggatagt tcccgccatc
     4081 aggctttctg cgacgtttgc aaccgcctgg aaactggtag cgcaagcgcg gctgacgctg
     4141 taagcatcgg tatgcacatt cattccggta ccgagaacaa tttcacgcgc aatgttgggg
     4201 gcttcaggca tttgtacgac ctgaccaaaa accagctgtt caatcacttc agcggggatc
     4261 tcgctgcgtg ccagcagttc gcctaccacc atcttcccta aatcaaccgc gggaatgcca
     4321 tgaaaagccg tcgcctggcg ggcaaaaggc gtacgtaaac cgctaacaat ggcgatacga
     4381 tcgccctggc gggtaaccag cggtaaaacc tgacccataa cactcccctg taaaaaaata
     4441 aataaagtgg tctgacctga tcatagtctt aaccattttt ttacatttag ccaagcggag
     4501 aaaaaggaaa gtgggagcta tgacacagtg aagacaggag aaagaaaaga aaacgcccct
     4561 gccagcaact gacaggggcg tacgtacccg agaggaatta acgcagaccc agctggaaga
     4621 tcagcatttc agcttcgcag gcaaaagtga aatcgatatc caggcgcaca ccgtcagact
     4681 cttcagtgaa agtcggggtg attttacacg gttcggattc cacgctacgg gctttttcag
     4741 tcagcgccgc cagcgtttgt tctgcttctg cgcggcttgc aaacacgcgg ctgtaagacg
     4801 cggtgcagtc ggagttgtcc ataatggtgc caacatccat acagcagcaa accggggttt
     4861 catcagcact acatttactc atcgttgatt tcctctgtat gtgcacccaa ggtgccagat
     4921 aaacgttgtg gatattttac gcttccggaa agtgctgctc cagttgttaa ttctgcaaaa
     4981 tcggataagt gaccgaaatc acacttaaaa atgatctaaa acaaaattca cccgaatccg
     5041 cgagtgcgca acctccaaat tttgccagct ggatcgcgtt tcttagatca tatttgaaaa
     5101 aagatggaaa catacttgca acattccagc tggtccgacc tatactctcg ccactggtct
     5161 gatttctaag atgtacctca gaccctacac ttcgcgctcc tgttacagca cgtaacatag
     5221 tttgtataaa aataaatcat tgaggttatg gtcatgagcc agaaaaccct gtttacaaag
     5281 tctgctctcg cagtcgcagt ggcacttatc tccacccagg cctggtcggc aggctttcag
     5341 ttaaacgaat tttcttcctc tggcctgggc cgggcttatt caggggaagg cgcaattgcc
     5401 gatgatgcag gtaacgttag ccgtaacccc gcattgatta ccatgtttga ccgcccgaca
     5461 ttttctgcgg gtgcggttta tattgacccg gatgtgaata tcagcggaac gtctccgtct
     5521 ggtcgtagcc tgaaagccga taacatcgcg cctacggcat gggttccgaa catgcacttt
     5581 gttgcaccga ttaacgacca atttggttgg ggcgcttcta ttacctctaa ctatggtctg
     5641 gcaacagagt ttaatgatac ttatgcaggc ggctctgtcg ggggtacaac tgaccttgaa
     5701 accatgaacc tgaacttaag cggtgcgtat cgcttaaata atgcatggag ctttggtctt
     5761 ggtttcaacg ccgtctacgc tcgcgcgaaa attgaacgtt tcgcaggcga tctggggcag
     5821 ttggttgctg gtcagattat gcaatctcct gccgggcaaa ctcctcaagg gcaagcattg
     5881 gcagctaccg ccaacggtat cgacagtaat accaaaatcg ctcatctgaa cggtaaccag
     5941 tggggctttg gctggaacgc cggaatcctg tatgaactgg ataaaaataa ccgctatgca
     6001 ctgacctacc gttctgaagt gaaaattgac ttcaaaggta actacagcag cgatcttaat
     6061 cgtgcgttta ataactacgg tttgccaatt cctaccgcga caggtggcgc aacgcaatcg
     6121 ggttatctga cgctgaacct gcctgaaatg tgggaagtgt caggttataa ccgtgttgat
     6181 ccacagtggg cgattcacta tagcttggct tacaccagct ggagtcagtt ccagcagctg
     6241 aaagcgacct caaccagtgg cgacacgctg ttccagaaac atgaaggctt taaagatgct
     6301 taccgcatcg cgttgggtac cacttattac tacgatgata actggacctt ccgtaccggt
     6361 atcgcctttg atgacagccc agttccggct cagaatcgtt ctatctccat tccggaccag
     6421 gaccgtttct ggctgagtgc aggtacgact tacgcattta ataaagatgc ttcagtcgac
     6481 gttggtgttt cttatatgca cggtcagagc gtgaaaatta acgaaggccc ataccagttc
     6541 gagtctgaag gtaaagcctg gctgttcggt actaacttta actacgcgtt ctgataacgc
     6601 gttcgcctgg ataaagtcac ctgcatagca ggtgacttta actcccccac ttcaccgaaa
     6661 gtagtgtcct cattgcttac ctcctgagtt ttgcaaacag cctgatggca gcttgccctt
     6721 tcaaatcaat aagcggtatc cacagacaaa tctgaaggga aaggcatatt ttcaggcgtt
     6781 ctgctcgtcc ttctcaaaga gtttactttt ctgaatttcc aggatactca cccccctggc
     6841 tattgtgcgc tcatacactc aaattaaaga taggttctaa ataaatgagc gttttttgat
     6901 agtctatttc attaggtaat atatatttgt aacaaatcaa tcaaaatgga ataaaatcat
     6961 gctaccatct atttcaatca acaataccag cgc
//
LOCUS       DAZGJW010000211         6921 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZGJW010000211 DAZGJW010000000
VERSION     DAZGJW010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.211"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..360)
                     /locus_tag="ACHKIK_004626"
     CDS             complement(<1..360)
                     /locus_tag="ACHKIK_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO9085646.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPNTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVP"
     gene            complement(609..2015)
                     /gene="gndA"
                     /locus_tag="ACHKIK_004627"
     CDS             complement(609..2015)
                     /gene="gndA"
                     /locus_tag="ACHKIK_004627"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO9085647.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(2126..2533)
                     /locus_tag="ACHKIK_004628"
     CDS             complement(2126..2533)
                     /locus_tag="ACHKIK_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HFO9085648.1"
                     /translation="MRLVDDNIMDELFRTAANSERLRAHYLLHASHQEKVQRLLIAFV
                     RDSYVEPHWHELPHQWEMFVVMQGQLEVCLYEQNGELQKKFVVGDGTGISVVEFSPGD
                     IHSVKCLSPKALMLEIKEGPFDPLKAKAFSKWL"
     gene            complement(2524..3732)
                     /locus_tag="ACHKIK_004629"
     CDS             complement(2524..3732)
                     /locus_tag="ACHKIK_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO9085649.1"
                     /translation="MKLALIIDDYLPHSTRVGAKMFHELGLELLSRGHDVTVITPDNT
                     LQAIYSVSMTDGIKVWRFKSGPLKDIGKAKRAINETLLSFRAWHALKHLIQHDTFDGI
                     VYYSPSIFWGGLVKKIKERCQCPSYLVLRDMFPQWVIDAGMIKAGSPIEKYFRYFEKK
                     SYQQADWIGLMSDKNLEIFRQANKGYPCEVLRNWASMTPVSAGDDYHSLRQKYALKDK
                     IIFFYGGNIGHAQDMANLMRLARNMMRHHDAHFLFIGQGDEVDLIKSLSAEWNLTNFT
                     HLPSVNQEEFKLILSEVDVGLFSLSSRHSSHNFPGKLLGYMVQSIPILGSVNGGNDLM
                     DVINKHRAGFIHVNGEDDKLFESAQLLLSDSVLRKQLGQNANVLLKSQFSVESAAHTI
                     EVRLEAGECV"
     gene            complement(3732..4862)
                     /gene="wecB"
                     /locus_tag="ACHKIK_004630"
     CDS             complement(3732..4862)
                     /gene="wecB"
                     /locus_tag="ACHKIK_004630"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO9085650.1"
                     /translation="MKKLKVMSVVGTRPEIIRLSRVLAKLDEYCDHLIVHTGQNYDYE
                     LNEVFFKDLGVRKPDYFLNAAGKNAAETIGQVIIKVDEVFEQEKPEAMLVLGDTNSCI
                     SAIPAKRRRIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINMTYSDIAREYLLAEGV
                     PADRIIKTGSPMFEVLTHYMSQIDGSDVLSRLNLTPGNFFVVSAHREENVDTPKQLAK
                     LANILNTVAEKYDIPVVVSTHPRTRNRINENGIQFHKNILLLKPLGFHDYNHLQKNAR
                     AVLSDSGTITEESSIMNFPALNIREAHERPEGFEEGAVMMVGLESARVLQALEIIATQ
                     PRGEVRLLRQVSDYSMPNVSDKVVRIIHSYTDYVKRVVWKQY"
     gene            complement(4859..5965)
                     /locus_tag="ACHKIK_004631"
     CDS             complement(4859..5965)
                     /locus_tag="ACHKIK_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HFO9085651.1"
                     /translation="MKILVTGAAGFIGRNLVFRLKEAGYNELIAIDRNSSLTDLEQGL
                     KQADFIFHLAGVNRPVKESEFEEGNCDLTQQIVDILKKNNKDTPIMLSSSIQAECDNA
                     YGKSKAVAEKIIQQYGETTNAKYYIYRLPNVFGKWCQPNYNSFIATFCHRIANDEAIT
                     INDPSAVVNLVYIDDFCSDILKLLEGANETGYRTFGPIYSVTVGEVAQLIYRFKESRQ
                     TLIIEDVGNGFTRALYSTWLSYLSPKQFAYTVPSYSDDRGVFCEVLKTKNAGQFSFFT
                     AHPGITRGGHYHHSKNEKFIIIRGSACFKFENIVTGERYELNVSSDDFKIVETVPGWT
                     HDITNNGSDELVVMLWANEIFNRSEPDTIARVLS"
     gene            complement(5967..>6921)
                     /locus_tag="ACHKIK_004632"
     CDS             complement(5967..>6921)
                     /locus_tag="ACHKIK_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFO9085652.1"
                     /translation="DITEIRIFSRDEKKQDDMRKEYNNSKLKFYIGDVRDYNSVLNAT
                     RGADFLYHAAALKQVPSCEFHPMEAVKTNVLGTENVLEAAIANGIKRVVCLSTDKAVY
                     PINAMGISKAMMEKVIVAKSRNLDSSKTVICGTRYGNVMASRGSVIPLFVDLIKAGKP
                     LTITDPNMTRFMMTLEDAVDLVLYAFEHGNNGDIFVQKAPAATIQTLAIALKELLNAH
                     EHPINIIGTRHGEKLYEALLSREEMIAAEDMGDYYRVPPDLRDLNYGKYVEHGDRRIS
                     EVEDYNSHNTERLDVEGMKELLLKLPFIRALRSGEDYELDS"
BASE COUNT         2041 a         1547 c         1326 g         2007 t
ORIGIN      
        1 gggtaccgtt gatttgatga ccataaccgc ataaggattt atctctacta cgtccttaat
       61 tactgattct acactcgatg tattgaaata attagtgtta ggatcatagt cggttggagt
      121 ggcgatgatg acataatcag catcccggta ggcttcattt ttatctaatg tggcattaaa
      181 gtgtatttta tctgattgca aaaactgctg aatttcctta tcaacaatag gagatatccg
      241 atcattcagc atagcaacgc gtgacggtaa aatatctaat gccacaacct catgattttg
      301 tgcgattaga agcccgtttg acaagcctac atagccggta ccggaaatgg tgattttcat
      361 ttcatgctct cagaattaac ttaactgtga atcatgatgt ttttagcatc ctgataagag
      421 ctaaaagttt taacgctacg ttgttcgatg gtcaacgcaa cgaaaaacaa ttcagatttt
      481 ctttataaga atagctgata tttattaagt taatattaag cagtgaaatt tagtctataa
      541 gataattggc aaaaaaaaag cccggcgtaa tatacgccgg gcctcaattt attgttggtt
      601 aaatcagatt aatccagcca ttcagtatgg aacacacctt ctttatcaat gcgcttataa
      661 gtatgcgcac caaaatagtc acgctgagcc tggatcaggt tcgcaggcag aacagcggca
      721 cggtagctgt cataataggc aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt
      781 actgcataag caacgacatc acgcagcgcc tgctggtagt catcggcaat ttgcttgaag
      841 tacggagcca acagcaggtt agcgatctga ggattttcgg cataagcatc agttattttc
      901 tgcaggaact gcgcacggat gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag
      961 ttcagatccc agttgtactc ttcagacgct gcgcgcagct gagagaagcc ctgagcgtaa
     1021 gaaacgattt tgcccagata cagcgcacgg cgaacttttt cgatgaactc agccttgtcg
     1081 ccagctggct gcgcttgcgg gccagagaga actttagatg cggcaacacg ctgatctttc
     1141 agagaagaga tataacgtgc aaacacagac tcggtaatca gcgacagcgg ttcaccgaga
     1201 tccagcgcgc tctggctggt ccatttaccg gtacctttgt tagccgcttc atccaggatc
     1261 acatcaacca ggtagttacc gtcttcatct tttttggtga agatgtcttt ggtgatgtcg
     1321 atcaggtagc tactcagttc accgttattc cactcggtaa aggtctgtgc cagttcttcg
     1381 ttagagagat tcaggccgcc tttcaggaga gaataggctt cagcaatcag ttgcatatca
     1441 ccgtattcaa taccgttgtg aaccatcttc acatagtgac ccgcgccatc agcaccaata
     1501 taggtaacgc acggttcgcc atcttcagca acggcggcga ttttagtcag gatcggtgca
     1561 accagttcat aggcttcttt ctgcccacca ggcataatgg aaggaccttt cagtgcgcct
     1621 tcttcaccac cggagacacc ggtaccaatg aagttaaaac cttctgcaga aagctcacga
     1681 ttacgacgaa tggtgtcctg gaagaaggta ttaccaccat caatgatgat gtcaccttta
     1741 tcgaggtatg gcttaaggga atcaatagca gcatccgtgc ctgcacctgc tttcaccatt
     1801 aacaggatgc gacgaggcgt ttccagagat tcaacaaact ctttcaccgt atagtaagga
     1861 accagtttct tgcctggatt ttcggcaatc acttcttccg tcttttcacg ggaacggttg
     1921 aaaatagaga cggtataacc acggctttcg atgttgagcg caaggttgcg ccccatcact
     1981 gccataccga ctacgccgat ctgttgcttt gacattgttt actcctgtca ggggtggtca
     2041 cgcggggtta cgcggcttga agaagctaag atggtaaccc agcatgtata gaataagata
     2101 gaagaataaa cggtggtgta tcgccctata accacttaga aaaagcctta gctttcagtg
     2161 ggtcaaatgg cccctccttt atctctaaca taagggcttt tggtgacagg catttgacac
     2221 tatgtatatc tccaggggaa aattccacga cacttattcc cgtaccgtct ccaacaacaa
     2281 actttttttg gagctcacca ttttgctcat acaaacaaac ttctaattgc ccttgcatga
     2341 cgacaaacat ttcccactga tgcggtaact catgccaatg gggttcaaca tagctgtcgc
     2401 gtacaaatgc aataagtaaa cgttgaacct tctcctgatg agatgcgtgc aataaataat
     2461 gagcgcgcaa acgttcagaa tttgctgcag tgcgaaaaag ttcatccata atattgtcat
     2521 caactaaacg cattctcctg cctccagtcg gacttcgata gtatgtgccg ccgattcaac
     2581 cgaaaattga gactttaaca acacattggc gttctgacct aactgttttc ttaaaactga
     2641 atcactaaga agcaattgtg cagattcaaa cagtttatca tcttcaccat taacatgaat
     2701 gaacccagcc ctgtgcttat taattacatc cattagatca ttaccgccat tcacactccc
     2761 aaggatcggg attgattgaa ccatataccc tagtaatttt ccggggaaat tatgtgaaga
     2821 atggcgagac gaaagggaga acaggccaac atcaacttca gataaaatta atttaaactc
     2881 ttcctggttc actgaaggta gatgagtgaa attagttaaa ttccattctg cagaaagaga
     2941 tttaatcagg tcaacttcat caccctgccc tataaacagg aaatgagcat catgatgacg
     3001 catcatatta cgcgcaaggc gcattaagtt tgccatatcc tgagcatgcc caatattacc
     3061 gccatagaaa aaaatgattt tatcttttag agcgtatttt tgacgaagtg aatgataatc
     3121 atcgccggca gacacaggag tcattgaggc ccaattacgt aaaacttcac acggataacc
     3181 tttattggcc tgacgaaata tctcaagatt cttatcagac attaacccaa tccagtcagc
     3241 ctgttgatat gatttttttt caaaatacct gaaatatttt tcaattggcg agccggcttt
     3301 tatcatacca gcatcaatga cccactgtgg gaacatatcc cttaggacca gatagcttgg
     3361 gcactggcat cgctctttaa tttttttaac caagcctccc caaaaaatag agggggaata
     3421 ataaacgata ccatcaaatg tatcatgttg gatgagatgc tttaatgcgt gccatgcacg
     3481 aaaagataaa agagtttcat ttatggcacg tttagcctta cctatatcct ttaaaggtcc
     3541 acttttgaaa cgccaaacct ttataccatc agtcatgcta acagaataga ttgcctgtaa
     3601 tgtgttgtca ggcgtaatta cagttacatc atggcctctg ctcagcaatt ccaggcctaa
     3661 ctcatgaaac attttagccc caactcgtgt gctatggggc aaataatcat caatgattaa
     3721 tgcaagtttc attagtattg cttccagaca acccgtttaa cgtagtcagt atatgaatgg
     3781 ataatacgca caactttatc tgaaacattt ggcatactat agtcactgac ctgacgaagt
     3841 aagcgtactt ctccacgagg ctgtgttgca ataatttcta atgcctgtaa cacacgggca
     3901 gactcaagac caaccatcat tacagcccct tcttcgaagc cttcagggcg ttcgtgcgct
     3961 tctcgtatat tgagcgcagg gaagttcata atggaggact cctctgtaat agtcccgcta
     4021 tccgataaaa cagcacgtgc atttttttgc agatggttgt aatcgtgaaa tcctaatggc
     4081 ttaagtagca agatattttt atgaaattga ataccgtttt cgttgatgcg gttacgagtg
     4141 cgcggatgag tagaaacaac taccgggata tcatattttt cagccacggt attaagtata
     4201 tttgccagtt tcgcaagctg tttaggggta tcaacatttt cttctctgtg ggcacttacc
     4261 acaaagaaat tcccaggtgt taaattcagg cgagaaagta catcggaacc atcaatctgc
     4321 gacatataat gagtgagtac ttcaaacatt gggctaccgg ttttaataat cctatcggct
     4381 ggtacacctt cagccaaaag atattcacgc gcgatatcac tatatgtcat attgatatca
     4441 gcggtgtggt caactatttt tctgttagtt tcttccggta cgcgttgatc aaaacaacga
     4501 ttcccagcct ccatatggaa aatcggaatt cttcgacgct ttgctggtat tgctgaaata
     4561 caggagttag tatcgccaag aactaacatg gcttctggtt tttcctgttc aaagacctca
     4621 tcaactttaa tgataacttg tccaatagtc tctgctgcat ttttacctgc ggcattaaga
     4681 aaataatcag gtttgcgaac acccaaatcc ttgaaaaaaa cctcattcaa ttcataatcg
     4741 taattttgcc cagtatgaac aataaggtga tcacaatatt catctaattt tgcaaggaca
     4801 cgcgagagtc gaataatttc tggacgagtc ccaacaaccg acatgacttt caattttttc
     4861 acgataaaac tctcgctata gtatctggtt cagaacgatt aaatatttca tttgcccaaa
     4921 gcataacaac tagctcatcc gagccattat tagtaatgtc atgcgtccat cccggaactg
     4981 tttcaacaat tttaaaatca tctgatgaaa cattaagttc atatcgttca cccgtgacaa
     5041 tattttcaaa tttgaaacaa gcacttcctc ggatgataat aaatttctca tttttggaat
     5101 gatgataatg ccccccccga gtaattcctg gatgcgcagt aaaaaacgaa aactggcccg
     5161 cgtttttcgt tttcaatact tcacagaata cccctctgtc atcactataa gaaggaaccg
     5221 tatacgcaaa ctgtttagga gaaaggtaac ttaaccatgt tgagtacaat gctcgagtga
     5281 atccattacc tacatcttcg atgattaatg tttggcgact ttctttaaat cgataaatta
     5341 attgtgccac ctcaccaaca gtaacagaat aaattggacc aaatgtcctg taaccagttt
     5401 cgttcgctcc ttctaatagc tttaatatgt cagaacaaaa gtcatctata tacaccaaat
     5461 ttacaactgc tgaaggatca ttaattgtaa tagcttcatc atttgcaatg cgatggcaga
     5521 aagttgctat aaaggagtta taatttggtt gacaccactt accgaataca ttcggcaagc
     5581 gataaatata atatttagcg tttgtcgttt ccccatactg ctgaatgatt ttttccgcaa
     5641 ctgctttact ctttccataa gcgttatcac attcagcttg gatggatgaa ctaagcatga
     5701 taggagtatc tttattattt tttttcagaa tatcaacaat ctgttgagtt agatcgcaat
     5761 tcccctcttc aaactcactc tccttcacag gacgatttac tccagcaaga tgaaaaatga
     5821 aatctgcctg cttaagtccc tgctctaaat ccgtcaaaga agagttacga tctatcgcaa
     5881 taagttcgtt atatccagct tccttaaggc ggaaaaccaa atttcgaccg ataaaccctg
     5941 cagcgccagt aactaaaatt ttcatattat gaatccaact cataatcttc accagaacga
     6001 agtgcccgga taaaaggaag ttttagcagt aattctttca ttccctcaac atctaatctc
     6061 tcagtattat gagaattata atcttccact tccgagatac gacggtcacc atgttccaca
     6121 tattttccat agttcaaatc gcggagatct ggtggaacac gataataatc acccatatct
     6181 tccgctgcaa tcatttcctc tcggctcaat aacgcttcgt aaagtttttc cccgtgtcga
     6241 gttccaataa tattgattgg atgctcatgg gcatttagca attccttaag tgcaatggct
     6301 aatgtttgaa ttgttgccgc aggtgctttc tgaacgaaaa tgtcaccatt atttccatgt
     6361 tcgaaagcat aaaggaccag atcgacagca tcctcaagcg tcatcatgaa acgagtcata
     6421 ttgggatcgg ttatggtcaa tggtttacca gctttgatta gatcaacaaa taatgggatg
     6481 accgatccac gtgaagccat tacatttcca taacgagttc cacagataac tgtttttgaa
     6541 ctgtcaagat tacgtgattt tgcaacaata actttttcca tcattgcctt agatatgccc
     6601 attgcattga taggataaac ggctttatcg gtactcaagc acaccacgcg tttaatccca
     6661 ttcgcaatag cagcctccag aacattttcc gtacccagaa catttgtctt aaccgcctcc
     6721 atagggtgaa attcacatga aggaacttgt ttaagggctg ctgcatgata cagaaaatcg
     6781 gcaccacgcg ttgcatttag aacggaatta tagtctcgca catcacctat ataaaatttt
     6841 aattttgagt tattatattc tttccgcata tcatcttgtt ttttttcatc cctgctaaat
     6901 attcgtattt cagtaatatc t
//
LOCUS       DAZGJW010000212         6794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZGJW010000212 DAZGJW010000000
VERSION     DAZGJW010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.212"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..238
                     /gene="yafQ"
                     /locus_tag="ACHKIK_004633"
     CDS             <1..238
                     /gene="yafQ"
                     /locus_tag="ACHKIK_004633"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFO9085653.1"
                     /translation="DVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKG
                     YRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            394..1134
                     /gene="dpaA"
                     /locus_tag="ACHKIK_004634"
     CDS             394..1134
                     /gene="dpaA"
                     /locus_tag="ACHKIK_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO9085654.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1105..1872)
                     /gene="yafJ"
                     /locus_tag="ACHKIK_004635"
     CDS             complement(1105..1872)
                     /gene="yafJ"
                     /locus_tag="ACHKIK_004635"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO9085655.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2078..2656)
                     /gene="lpcA"
                     /locus_tag="ACHKIK_004636"
     CDS             complement(2078..2656)
                     /gene="lpcA"
                     /locus_tag="ACHKIK_004636"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO9085656.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2896..5340
                     /gene="fadE"
                     /locus_tag="ACHKIK_004637"
     CDS             2896..5340
                     /gene="fadE"
                     /locus_tag="ACHKIK_004637"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO9085657.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTASLGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5383..5829)
                     /gene="ivy"
                     /locus_tag="ACHKIK_004638"
     CDS             complement(5383..5829)
                     /gene="ivy"
                     /locus_tag="ACHKIK_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO9085658.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6010..6777
                     /gene="yafV"
                     /locus_tag="ACHKIK_004639"
     CDS             6010..6777
                     /gene="yafV"
                     /locus_tag="ACHKIK_004639"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO9085659.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
BASE COUNT         1578 a         1731 c         1838 g         1647 t
ORIGIN      
        1 ggatgtaaaa cttgcacaaa agcgtcataa ggatatgaat aaattgaaat atcttatgac
       61 gcttcttatc aataatgctt taccgcttcc agctgtttat aaagaccacc cgctgcaagg
      121 ttcatggaaa ggttatcgcg atgctcatgt cgaaccggac tggatcctga tttacaaact
      181 taccgataaa cttttacgat ttgagagaac tggaactcac gcggcgctct ttgggtaaag
      241 cacgctgccg atgaaatttg taaaccctgc gccgacaggc ttgcgtaaaa ctcttttggt
      301 gataatgtga gccttccaaa tccagactat tccgatttgg gatctatttt gccgtcctgg
      361 cgtgtgtaac cgttttatca aggaataagc agtatgcgta aaatcgcatt aattcttgcg
      421 atgttgttga tcccgtgcgt ttcgtttgcc ggtttgctgg gcagcagtag ctcgaccacg
      481 ccagtgagca aagagtataa gcagcagttg atgggatccc ctgtctacat ccagatcttc
      541 aaggaagaac gtacgctcga tctctacgtc aaaatgggcg agcaatatca gctgctcgac
      601 agttataaaa tctgtaaata ttccggcggc ttagggccaa aacagcgtca gggcgatttc
      661 aaaagcccgg aagggtttta tagcgtccag cgtaatcagt taaaaccaga cagccgttac
      721 tacaaagcga ttaatattgg tttccccaat gcctatgacc gtgcgcatgg ttatgaaggg
      781 aaatacctga tgatccacgg cgattgtgtt tccatcggct gctacgcaat gaccaatcag
      841 ggtattgatg agatcttcca gttcgttact ggtgcgctgg tgtttggtca gccgagcgtg
      901 caagtgagta tttacccgtt ccgcatgacc gacgccaata tgaagcgcca taaatattcc
      961 aactttaagg acttctggga gcagctgaag ccgggctacg actactttga gcaaacccgt
     1021 aagccaccga ccgtttctgt ggtcaatggc cgttacgtgg tcagcaagcc gttaagccac
     1081 gaagttgtgc agccacaact ggcatcaaac tacacgctcc ccgaggcaaa ataagcgcca
     1141 ttcgcctggc ataatctttt gccaggtttc attgcccgtc agcggctgtg ttgcaatcac
     1201 cgtgaccaca tcattcggtg tggtctgcga gctgaagtcg atttccacat cctgatccag
     1261 cagcgttgcc acgccaaacg gcgcgcggcg ggtgatccag tgtaaattag tcgagcaata
     1321 cgccatcaca tagcgcccgt cagaaagcag catgttgaaa acgcccttct gccgcagttc
     1381 atccgccagt gaggcgatat atttaaacac tgccgccatg ttgcccggtg tgcgcgggta
     1441 acgctgcgtt aatttatgca ggagccagca gaaggctttt tcgctatcgg tttcaccgac
     1501 cgggcggaag ttgccggttt ccagtgattt gtagcccgtc agttgtccgt tatgggcata
     1561 agtccagtta cgcccccata actcgcgggt aaatgggtga gtattttcca gcgccacctc
     1621 gccccgatta gcctggcgaa tatgagccac caccgaacag gattttatcg ggtagtcctg
     1681 gacaagtttg gcgatggggg aattaaagct gggttgtgga tctttaaatg tgcgacagcc
     1741 tttaccttcg taaaaggtaa tgccccagcc atctttatgt ggcccggttc caccaccacg
     1801 ctgtacaagt ccggtgaaac taaagcagat atcggtaggg acgttggcgc tcatcccgag
     1861 caattcgcac atgggtaacc tccacaacag cgggagcgaa tggcccccgg cgaagattga
     1921 tttaagaatt ttcaggtcgg atgcggcgta aacgaattat tcggcctacg gttcgggtac
     1981 agatttgtag gcatgataag acgcgtcagc atcgcatcag gcatcagcgc acaagtgccg
     2041 gatgcggcgt aaacgcctta tccagcctac gccagactta cttaaccatc tctttttcaa
     2101 ttaactggat caggatatgg atcactttaa tgtgaatctc ctgaatgcgg tcggcataac
     2161 caaagtgcgg tacgcgaatt tcgatatccg ccgtgccagc cattttgccg ccgtctttac
     2221 cggtcagggt gatcactttc atccccttct cacgcgccgc tgcgatcgct ttgatcacgt
     2281 ttgcagagtt accggaggtg gagatcccca gcagtacatc gccttcgcga cctaccgctt
     2341 caacgtagcg ggagaaaata tcattgaaac cgaaatcatt accgacgcag gaaatatgac
     2401 taacgtcaga aatagcaatc gccgggtagc ccggacggtt ttcacggtag cgaccggtca
     2461 actcttcggc aaagtgcata gcgtcgcaat gggaaccgcc gttgccgcag gaaagcactt
     2521 tgccaccggc tttaaagctg tctgctaaca ggaccgccgc gcgctgaatg gcgtgaatat
     2581 tggcgtcatc ttttaaaaag ttagccagcg tttccgccgc ttcgttcagt tcgttacgaa
     2641 taagatcctg gtacatgagg atatccttca gcataaatgt aatagacaaa atgcagtgta
     2701 ccggataccg ccaaaagcga gaagtacggg caggtgctat gaccaggact ttttgacctg
     2761 aagtgcggat aaaaacagca acaatgtgag ctttgttgta attatattgt aaacacattg
     2821 ctaaatgttt ttacatccac tacaaccata tcatcacaag tggtcagacc tcctacaagt
     2881 aaggggcttt tcgttatgat gattttgagt attctcgcta cggttgtcct gctcggcgcg
     2941 ttgttctatc accgcgtgag cttatttatc agcagtctga ttttgctcgc ctggacagcc
     3001 tccctcggcg ttgctggtct gtggtcggcg tgggtactgg tgcctctggc cattatcctc
     3061 gtgccattta actttgcgcc tatgcgtaag tcgatgattt ccgcgccggt atttcgcggt
     3121 ttccgtaagg tgatgccgcc gatgtcgcgc actgagaaag aagcgattga tgcgggcacc
     3181 acctggtggg agggcgactt gttccagggc aagccggact ggaaaaagct gcataactat
     3241 ccgcagccgc gcctgaccgc cgaagagcaa gcgtttctcg acggcccggt agaagaagcc
     3301 tgccggatgg cgaatgattt ccagatcacc catgagctgg cggatctgcc gccggagttg
     3361 tgggcgtacc ttaaagagca tcgtttcttc gcgatgatca tcaaaaaaga gtacggcggg
     3421 ctggagttct cggcttatgc ccagtctcgc gtgctgcaaa aactctccgg cgtgagcggg
     3481 atcctggcga ttaccgtcgg cgtgccaaac tcattaggcc cgggcgaact gttgcaacat
     3541 tacggcactg acgagcagaa agatcactat ctgccgcgtc tggcgcgtgg tcaggagatc
     3601 ccctgctttg cactgaccag cccggaagcg ggttccgatg cgggcgcgat tccggacacc
     3661 gggattgtct gcatgggcga atggcagggc cagcaggtgc tggggatgcg tctgacctgg
     3721 aacaaacgct acattacgct ggcaccgatt gcgactgtgc ttgggctggc gtttaaactc
     3781 tccgacccgg aaaagctgct gggtggcgca gaagatttag gtattacctg tgcgctgatc
     3841 ccgaccacca cgccgggcgt ggaaattggt cgtcgccact tcccgctgaa cgtaccgttc
     3901 cagaacggac cgacgcgcgg taaagatgtc ttcgtaccga tcgattacat catcggtggg
     3961 ccgaaaatgg ccgggcaagg ctggcggatg ctggtggagt gcctctcggt aggccgcggc
     4021 atcaccctgc cttccaactc aaccggcggc gtgaaatcgg tagcgctggc aacgggcgcg
     4081 tatgctcaca ttcgccgtca gttcaaaatc tctatcggta agatggaagg gattgaagag
     4141 ccgctggcgc gtattgccgg taatgcctac gtgatggatg ctgcggcatc gctgattacc
     4201 tacggcatta tgctcggcga aaaacctgcc gtgctgtcgg ctatcgttaa gtatcactgt
     4261 acccaccgcg ggcagcagtc gattattgat gcgatggata tcactggtgg caaaggcatt
     4321 atgctcgggc agagcaactt cctggcgcgt gcttaccagg gcgcaccgat tgccatcacc
     4381 gttgaagggg ccaacattct gacccgcagc atgatgatct tcggacaagg agcgattcgt
     4441 tgccatccgt acgtgctgga agagatggaa gcagcgaaga acaatgacgt caacgcgttc
     4501 gataaactgt tgttcaaaca tatcggtcac gtcggtagca acaaagttcg cagcttctgg
     4561 ctgggcctga cgcgcggttt aaccagcagc acgccaaccg gcgatgccac taaacgctac
     4621 tatcagcacc tgaaccgcct gagcgccaac ctcgccctgc tttctgatgt ctcgatggct
     4681 gtgctgggcg gcagcctgaa acgtcgtgag cgcatctcgg cacgtctggg ggatatttta
     4741 agccagcttt acctcgcctc tgccgttctg aagcgttatg acgacgaagg ccgtaatgaa
     4801 gccgacctgc cgctggtgca ctggggcgta caagatgcgc tgtatcaggc tgaacaggcg
     4861 atggatgatt tactgcaaaa cttcccgaac cgcgtggttg ccgggctgct gaatgtggtg
     4921 atcttcccga ccggacgtca ttatctggca ccttctgaca agctggatca taaagtggcg
     4981 aagattttac aagtgccgaa cgccacccgt tcccgcattg gtcgcggtca gtacctgacg
     5041 ccgagcgagc ataatccggt tggcttgctg gaagaggcgc tggtggatgt gattgccgcc
     5101 gacccaattc atcagcggat ctgtaaagag ctgggtaaaa acctgccgtt tacccgtctg
     5161 gatgaactgg cgcacaacgc gctggcgaag gggctgattg ataaagatga agccgctatt
     5221 ctggtgaaag ctgaagaaag ccgtctgcgc agtattaacg ttgatgactt tgatccggaa
     5281 gagctggcga cgaagccggt aaagttgccg gagaaagtgc ggaaagttga agccgcgtaa
     5341 ttgaataacg gagccgaaag gctccgtttc tttatccgct aattatttaa aattaaagcc
     5401 atccggatgg ttttccaggc tgccggtcaa cgccgcgaac agcaccgttt taccatcaat
     5461 cgaaagcgca tcgttcacat tcagccaggt gagtttctct tgcgacgttt tctcatcaat
     5521 agtcgagaac agccccgtca tctgattaga tttctcggac cacatcacag cgatacgttg
     5581 cgagccacag tcatgcggtt tgcacgcgct catcacctga tacgtctcat ctcccaacgt
     5641 tacggtttgt gcgggagtat aagtaccgcc tttcatcacc caggcaggca gcttatgccc
     5701 ttgtaccatc tgattaaatg cagctttggt ggtttcgccc tttgcaaggc tgctaatggt
     5761 taaatcatcc tgcgccattg cactggtggc gatgaccaga gcggcgactg tcgttattgc
     5821 cttaaacatc attcctcccg agcttatcct gcccatgtta ttaaccttct aaagcctggc
     5881 gataacacga taatttgcaa atcagatatt tccaatatca gcatttgtta tcaatttatt
     5941 ctgcgctaag gcaactgcct gataaggtgt taaagttata aaacccttcg cataaaggag
     6001 ttgtacaccg tgcctggttt gaagattacg cttttgcagc aaccactggt gtggatggat
     6061 ggccctgcca acctgcgtca ttttgatcgt caactggaag gtattaccgg gcgcgatgtg
     6121 atcgttctac cggagatgtt taccagcggc tttgccatgg aagcggcagc ttcgtcgcta
     6181 gcacaagatg acgtagtgaa ctggatgaca gctaaggcgc agcagtgcaa tgcgctgatt
     6241 gcaggcagtg ttgcattaca aacggagtct ggttcggtta accgcttttt gctggttgag
     6301 ccgggcggca cggtacattt ttatgataag cgtcatctgt tccgcatggc agatgagcat
     6361 ctacattata aagcgggcaa tgcgcgagtg attgtggaat ggcgcggctg gcgtattttg
     6421 ccgctggtgt gctacgactt acgttttcct gtgtggtcgc gcaatctcaa cgattatgac
     6481 ctcgccctgt acgtcgccaa ctggcctgct ccgcgctctc tgcactggca ggcattgctg
     6541 acggcccgcg cgattgagaa tcaggcgtat gtggcgggat gcaatcgcgt cggcagcgat
     6601 ggcaacggct gccattatcg cggtgacagc cgggtgatta atccacaagg cgagattatc
     6661 gctactgccg acgcgcatca ggcaacgcgc attgatgccg agctgtcgat ggcggtgtta
     6721 agggaatatc gcgataagtt tccggcatgg cgggatgcgg atgagtttag gttgtagtga
     6781 gtgggggctg tatt
//
LOCUS       DAZGJW010000213         6762 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZGJW010000213 DAZGJW010000000
VERSION     DAZGJW010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.213"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(178..1107)
                     /gene="fdhE"
                     /locus_tag="ACHKIK_004640"
     CDS             complement(178..1107)
                     /gene="fdhE"
                     /locus_tag="ACHKIK_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO9085660.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1104..1739)
                     /gene="fdoI"
                     /locus_tag="ACHKIK_004641"
     CDS             complement(1104..1739)
                     /gene="fdoI"
                     /locus_tag="ACHKIK_004641"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO9085661.1"
                     /translation="MKRRDTILRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1736..2638)
                     /gene="fdoH"
                     /locus_tag="ACHKIK_004642"
     CDS             complement(1736..2638)
                     /gene="fdoH"
                     /locus_tag="ACHKIK_004642"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO9085662.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2651..5701)
                     /gene="fdnG"
                     /locus_tag="ACHKIK_004643"
     CDS             complement(2651..5701)
                     /gene="fdnG"
                     /locus_tag="ACHKIK_004643"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5114..5116),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO9085663.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            5895..6728
                     /gene="fdhD"
                     /locus_tag="ACHKIK_004644"
     CDS             5895..6728
                     /gene="fdhD"
                     /locus_tag="ACHKIK_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO9085664.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1479 a         1833 c         1832 g         1618 t
ORIGIN      
        1 atacgcttgt catcagtgac atacccataa ccttatcgat gtgttggagg gaagaacgag
       61 gcaaacagac aattgcggca tgatcttgaa tgggtaacgc ggaatacgag agatggtgtc
      121 gtcacaccct atgagctgta atccgggcga ccagcgccac ccggaaaacg tcagagatta
      181 ctccccttca cccggaaaca ggaacgggtt gatggaactg cgggcatagc cttcttgctc
      241 cattcgcgcg tccagtacca gagaggcgag gtcatctgcc acggcttcaa ctttcggttc
      301 tttttcctga taaagaatct tcaggtaagt gccgcagtca tcgcagcttt cggctttaat
      361 cgcggcctgt tcgtcatcca gcgaccagta atgcagtttg ccgctctgct cgcagttgct
      421 gcattttacg cgcactacgt gccattcggt ttcacacagg ttgcagtgca ggtaacgcag
      481 accctgagtg gtgccaattt gcaccatgct ggacaccggc atagaaccac aaaccgggca
      541 atattgacgt tgctcgccgt attcagcgcg ggctttgccg gggatcagat tggccatctg
      601 cgcccagtag agcgacagtg cagcccagat aaacggcgct ttatcgctgc tgacggatga
      661 gaaatcagag gcaaacagtg cgctggccat atcttccagc tcctgagtcg atgccttctc
      721 cagattctca atcactgcca gcgccgggcc gctcatttca ggtttcagct cagcaatcag
      781 cgccatcagc agcttttgcc agtgcttatc acgcggcaga acgtgaatat ccagcggggg
      841 cttgccctgt gcgctggctt ctttaatgcg cgcggtcaga tccatctcca gcggatggtc
      901 gtacagcacc acttcctggg cgtgggcgat aagcgcagca aagcgcaggt aatcacccag
      961 cggattattt tctgccagct cgcgcagacg ctcggcgcgg cggttgtata aattcttgag
     1021 ccgagggaac aataacggcg gaatcatatc cgccgtacgt ttctcgctcg aacccagctc
     1081 atcttgcggg attatgcgaa tactcattca gctttctttt ccgttgtctt gcggacctca
     1141 cggtaccagc gcgggtgatg tttcttcgcc caggcgctgg taacccatcc ttccaccatg
     1201 gcggtaatcg tgcctttcac ccaaagggcg gcgtagatat gcaccatgat aaccacaatt
     1261 aacgccactg cggcaaatga atgcagcatt aacgcgaatc ggatcaccgg gattgagaaa
     1321 gcaggcgcaa aataaggacg ccagataatc acgccgctca ccagcagcag aaccaggaaa
     1381 ataatcgccg cccagaaaac gcatttctga ccgaagttat aacgcccggt gtcacctact
     1441 tcctcgttga cgacgatctt acgaatattc ttcgcccaaa agatatcatc ccgattgatt
     1501 aggttgtgat gccagtaacg gaaaaacatg atgatgaacg aggcaaacat aaccacgccg
     1561 acaaacgggt gcagaattcg cgccagctgc ggtgtgccca tgatttgcat caaccagttg
     1621 aaggacggga acaaaaagcc cagcccgctc accgccgcca ggatgaagca gaaggcggtg
     1681 atccagtggt tgatacgttc cggcgcggtg tagcgcagga tggtgtcacg tcgtttcatt
     1741 tgcgctcctc gtctttctct tcgtgcagat tattctcttc ctcatccgca cggttcggac
     1801 cgacacccac gtagtggaag atactggccg caaaggtagc cgcaaagcca acagccgcga
     1861 gcggtttcca gatgcctttc cagaatttca cggtttcgct gatttccggg ttctccggca
     1921 agccatgata cagatttggc ttgtcagcat ggtgcagcac gtacattacg tgtgtaccac
     1981 cgacgcctgc cggatcgtac agacccgcat tgtcgtaacc gcgggttttc agctcagcaa
     2041 cgcgctcgct cgccagcgtt ttcatcgact ctttcgtacc gaagtgaatc gcgcccgttg
     2101 ggcaggtctt cacgcaggct ggttcttgcc caaccaccac gcggtcaacg cacagcgtac
     2161 atttgtagac gcggttgtct tccgggttga ggcgcggaat gtcgaacgga cagcccgcaa
     2221 tgcaataacc gcagccaatg cactgctcgg actggaagtc gacgataccg ttggcatact
     2281 gaataattgc accttccgcc gggcacgctt tcaggcagcc tggatcggaa cagtgcatac
     2341 agccgtcttt gcggatcagc cattccagtt tgtcgttctg ctccacttcc gagaagcgca
     2401 ttaccgtcca cgatttggcg cttaaatcat tggggttgtc gtacacccca atgttattgc
     2461 cgacggtatc gcgaatgtcg ttccactctg aacacgccac ctgacaggct ttacagccga
     2521 tacaggtggt gacgtcgatg agtttcgcca cttcttcctg gaagtcccgc gcctgaggcg
     2581 cgggggtcag accgttagtc gcggaacgac gaatgatatc ttgagattga taagccatat
     2641 gtcgtctccg ttacaccttt tccacattca caaggaagga cttaaactcc ggcgtctgcg
     2701 tgttcgcatc accaacgaat ggcgttaacg tattggcaat aaagcctttt ttagctacgc
     2761 cttcatagcc ccagtgaatc ggaataccga tggtatcgat atctttgccg ttcgctttca
     2821 gtgtacgaat acgtttggtc accaccgctt tggccttgat atagccacgg ttggaggaga
     2881 ctttcacggt atcgccctgg gcaatgccaa gtttattcgc cagcgactcc ccgatttcca
     2941 caaactgctc tggttgcaaa atcgcgttca acagcgcgtg tttggtccag tagtggaaat
     3001 gctcggtcag acgataggtg gttccgacat acgggaactt atcggcttta cccaatgctt
     3061 cggcgtcgtc tttaaagata cgtgcagccg ggttcgagat aacgtttgga tgcagcgggt
     3121 tagttcccag cggcgtttca aacggctcgt agtgttccgg gaacggacct tccgccatct
     3181 tatcgagggc aaacagacgc cccatgcctt cctgctgcat gataaacggc ccgacgccgc
     3241 tacccggagg cgctgcgctg tagtccggaa tatcccagcc ggtccactta gtgccgtccc
     3301 atttcagcaa ctgacgcttc ggatcccacg ggttaccctg cggatctgcg gaggcgcggt
     3361 tatacagaat gcggcggtta agcggccatg cccatgccca gcccagcgtg ttaccgaggc
     3421 cagacggatc ggcgttatca cgacgcgcca tctggttgcc ttccggcgtc cagctaccgg
     3481 cgaaaatcca gcagccacag gaggttgtac cgtcatcgcg caattgggcg aacgagctaa
     3541 gttgttggcc tttcttgacg ataaccgcac cggttgccgg atcggtaata tcggccagcg
     3601 ccttaccgtt gctctccatc gctacttctt ccgacttcgg ctcatgcgga atggcgtagt
     3661 tccaggtcat gttcagcacc tgatccgggt tcgcgccacc ctgttcggca tacatcttgc
     3721 gcaagcgcag gaagataccg gagaggatct caccgtcagt cagcgcaatc cccggggcgt
     3781 ccgcaccttt ccagtgccac tgcaaccagc gacctgagtt aacgattgaa ccgttctctt
     3841 ctgcgaagca ggtcgatggc agacggaaca cttcggtctg gatcttcgac gagtcaactt
     3901 cgttcagctc accgtggttc tgccagaagt tagaggtttc agtgttcagc gggtcgatgg
     3961 tgacgaggaa cttcagtttc gacagacaac cgatcacttt gtttttgttc gggaatgagg
     4021 caacagggtt aaagccctgg cagatatagc cattgacctt gccttctttc atcatctcga
     4081 agtattgcag gacgtcgtag cctttatccc acttcggcaa ccagtcaaag ccccagctat
     4141 tttccgccgt cgctttatca ccaaagaagg ccttcatcat agagacgaag aatttcgggt
     4201 agttgcccca gtagtttacc tggccttcca gcagcggttt tggcgtgttg gcggtaaggt
     4261 aggtttgcag atcggtctgc ttctcgcttg gcagcgtcat gtaacctggc agactctgcg
     4321 acagtagccc caggtccgtc agcccctgaa tattggagtg accgcgcagg gcgttaacgc
     4381 cgccgccggc catccccata ttgccgagca gcagctggat catcgccatc gtacgaatgt
     4441 tttgcgcacc aacggagtgt tgcgtccagc cgagggcata caggaacgag gcagttttat
     4501 cgtgagcact ggtttctgcg atgtattcgc agactttcag gaacgcgtct tttggcgtac
     4561 cacagatgtt ttcaaccaca tctggcgtgt agcgggaaac gtgctgtttc agcaagttcc
     4621 acacgcagcg cggatgttgc agcgtggtat cgcgtttggc gaagccgttt tcgtccagtt
     4681 cataagtcca ggaggattta tcgtacttgc gtttttccgc gtcgtagccg gtgaacaggc
     4741 catcttcaaa gccgtaatcc tcacgcacta tcaggctggc gttggtatag gcttcggtgt
     4801 attcgcggtt gaatttttca ttgttcagca ggtacagcaa tacgcctgac aggaaagcaa
     4861 tgtcagtacc ggaacgaata ggggcatagt agtcagctac cgccgccgta cgcgtaaagc
     4921 gaggatcgat cacaatcagc ttcgcgccgt tgtgaatttt ggcttccatc gcccagcgga
     4981 acccgaccgg gtgagcttca gcggcgttac cgcccatcac cacgacgagg ttggcgttct
     5041 tgatgtcgac ccagtggttg gtcatcgcac cgcgaccaaa tgttggagca agacttgcta
     5101 ccgttggtcc gtgtcagaca cgcgcctggt tgtcgaccgc gagcataccc agcgcgcggg
     5161 agaatttttg cgttaaatag ccggtttcgt tactcgacgc ggaagcacac agcatcccgg
     5221 tggagagcca gcggttaaca gtcacgcctt cggcgttttg cgcaatgtag ttagcatcgc
     5281 ggtcttcttt catcagtttg gcgatgcgat caaacgcctc ttcccaactg atttgctgcc
     5341 atttatcaga acctggcgca cggtattccg gaaacttcag acggctttcg gagtggatga
     5401 aatccaccag gccagcgcct ttcggacaaa gtgcaccgcg gttgaccggg tgatccggat
     5461 cgccttcgat atggaagata gatgctttgg cgttttttgc tccgtcaccg aggctgtaca
     5521 tcaacagccc acagcctacg gaacaatagg tgcaggtatt acgggtttcg cgggtgcgca
     5581 gcagtttata ctgccgggtt tccgcgagcg ctacgctggg tgcaaaaccc agtgccgctg
     5641 ccgtggtgcc tgccataccg ccagcgcaga tcttaaagaa ctgccttctg ctgacctgca
     5701 tggattgctc cttgtttcga cattgtcacg tcccatttac attcgcttgc tgcgtgtgca
     5761 gggagtggga gttatttttc tttgcggaag gggccgcaaa ggtccagaat tggctcaatt
     5821 tccctccatc caggaagggt ttgtaacaga ataccataat gttggtgtgt gtgttcttat
     5881 ctggttaaga gaaagtgaaa aaaacacagc aaaaagaaat cgaaaatgtg acaaatatca
     5941 caggtgttcg tcaaattgag ttatggcggc gtgatgattt acaacaccca cggttagatg
     6001 aggtcgcgga agaagttccc gttgcgctgg tctacaacgg catttcgcat gtggtgatga
     6061 tggcgtcgcc caaagacctt gagtactttg cgctcggttt ttcgctttcc gaggggataa
     6121 tagaaagtcc gcgcgatatc ttcggcatgg atgtcgttcc ttcctgtaat ggtcttgaag
     6181 tacaaattga gctttccagc cgccgcttta tggggttgaa ggagcgccgc cgggcgctgg
     6241 cgggacgtac gggctgtggc gtatgcggcg tggagcaact taatgacatc ggaaaaccgg
     6301 tgcagccgct accgttcacc cagacgtttg atctcaacaa actggatgat gcattacgtc
     6361 atctcaacga ttttcagcca gtggggcaac tgactggttg tactcacgcc gctgcctgga
     6421 tgttgccatc tggcgaactg gtcggcgggc atgaagacgt gggtcgccat gtggcgctgg
     6481 acaaactgtt aggccgccgg tcacaagaag gggaaagctg gcagcaaggt gcggtactgg
     6541 tttccagtcg tgccagttat gaaatggtgc aaaagtcggc gatgtgcggc gtagagattt
     6601 tgtttgcggt gtctgccgcg accacgcttg ctgtagaagt ggctgagcgt tgtaatctga
     6661 cgctggtggg tttttgtaag ccgggtcggg caacggttta tacccatccg cagcgtttga
     6721 gcaattaatt aaaataagtc ctgcgatata tattaaattg ca
//
LOCUS       DAZGJW010000214         6434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZGJW010000214 DAZGJW010000000
VERSION     DAZGJW010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.215"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..397
                     /gene="ydjO"
                     /locus_tag="ACHKIK_004645"
                     /pseudo
     CDS             1..397
                     /gene="ydjO"
                     /locus_tag="ACHKIK_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(401..1792)
                     /gene="tcyP"
                     /locus_tag="ACHKIK_004646"
     CDS             complement(401..1792)
                     /gene="tcyP"
                     /locus_tag="ACHKIK_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO9085665.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1925..2515)
                     /gene="ydjM"
                     /locus_tag="ACHKIK_004647"
     CDS             complement(1925..2515)
                     /gene="ydjM"
                     /locus_tag="ACHKIK_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO9085666.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2678..3346)
                     /gene="hxpB"
                     /locus_tag="ACHKIK_004648"
     CDS             complement(2678..3346)
                     /gene="hxpB"
                     /locus_tag="ACHKIK_004648"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO9085667.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3493..4029
                     /gene="yniB"
                     /locus_tag="ACHKIK_004649"
     CDS             3493..4029
                     /gene="yniB"
                     /locus_tag="ACHKIK_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO9085668.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4070..4930)
                     /gene="yniA"
                     /locus_tag="ACHKIK_004650"
     CDS             complement(4070..4930)
                     /gene="yniA"
                     /locus_tag="ACHKIK_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO9085669.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5036..5326)
                     /gene="ghoS"
                     /locus_tag="ACHKIK_004651"
     CDS             complement(5036..5326)
                     /gene="ghoS"
                     /locus_tag="ACHKIK_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO9085670.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5427..6356)
                     /gene="pfkB"
                     /locus_tag="ACHKIK_004652"
     CDS             complement(5427..6356)
                     /gene="pfkB"
                     /locus_tag="ACHKIK_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO9085671.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1669 a         1599 c         1573 g         1593 t
ORIGIN      
        1 tatttaagga ctatatacta tgaaaaaaat tatatacatc ctgccgggag catttttgct
       61 gcgattcaaa aaaaatgaga gtctcctaaa gtttccttcg ataatatcaa tgcttaatat
      121 caatcttctt tttaatccac taaatcttcc gggaatggga agtggtgtgc ttgaagatat
      181 tatgagtatt ccagatagtt cacttagaaa acgtctcggg tatgaggttt taagcttctc
      241 actacaggca cattcattga gccaggaatg tattgataag ttggacattt ttttcgctga
      301 cgatctgttt aaatatgagt cagtatgcat cgccgcgatg gaacatttaa aaagtaaagc
      361 aacggcccct atacaaaacg gaccgttgcc agcataagaa ttaacggtgt gccagttcgg
      421 cgtcgtcttc gctatccaga atggctttat cggtttgctt cagccactgg ctggtcagcg
      481 tgccagctgt catcgagcca ctaacgttta gcgccgtacg gcccatgtcg ataagcggtt
      541 caacggagat taacagcgcc accagggtta ctggcaggcc catcgcaggc agcacaatca
      601 gtgcggcgaa agttgcacca ccaccgacac cggcaacgcc tgcggaacta acggtaacaa
      661 taccgaccag cgtcgcaatc cacatcgggt ccagcgggtt aatgccaacc gtaggcgcaa
      721 ccatcaccgc cagcattgcc ggatacaaac cggcgcagcc gttctgacca atggttgcac
      781 cgaaagaggc ggcgaaactg gcgatggatt caggaacgcc cagacgacgc gtttgtgctt
      841 ccacattcag tgggatagac gcagcactgg aacggctggt aaaggcaaac gtcagcacag
      901 gccatacctt acggaagtac ttcagcggac tcacgccatt aatgcccagc agaatgccat
      961 gcactgcaaa cataatcagc agaccgaggt aggacgcgac aacgaaactt cccagtttga
     1021 tgatgtcttg caggttagaa cctgcaacca ctttggtcat tagtgccaga acgccgtaag
     1081 gggtcaactg catgaccagg cgaaccagtt tcatcaccca gctttgcagg gtatcgatag
     1141 cggttaagac gcgttcacct ttcggcgcat catccttcag cagtttcagc gcagctacac
     1201 cgaggaatgc ggcaaaaatt accacgctga taattgacgt cggattggct ccggtcagat
     1261 cggcaaacgg gtttttcggg ataaaggaca agaccagctg cggaacgctc aggtcagaga
     1321 ctttaccaac atagttactt tcgatggcgt tcagacgtgc agtttctgca cctccctgaa
     1381 ccagaccttc agccgtcaaa ccaaacaggt tggtgaccag cacaccgacc agcgccgcaa
     1441 tcagtgtggt aaacaaaagc gtaccgatgg tcagaaaact gattttgcct aactgagatg
     1501 cgttatgcag acgggcaacc gcgctcagaa tagaggcgaa gactaacggc ataacgatca
     1561 tttgcagcag ttgaacatag ccgttaccaa cgatgttaaa ccactgtaca gaatctttaa
     1621 gtacctggct gtcagaacca taaatggtat gcagggcaag gccaaaaacc acacccatca
     1681 ccagacccac cagcactttt ttcgccagac tccactgttt gtggcgggtc tgagccagcg
     1741 caaacagcag tacaacgaac accacgatgt tcgcaattaa tggaaagttc atccccgttc
     1801 tcctgatctt attatttcta tcactgcgta gcagtcatag tgttggcgca agattaacag
     1861 aactgtgagc tggctcttat attcaaatgg aattgcttat tccagaaagg tgatttttgc
     1921 ctttttagta ttcaacctga tgcttaataa gccgatgaaa ctggatctgc aaggtattga
     1981 tcatttgcga tgaccaacga accgcgctgt tttcgggtaa tgaatggggc atccataccg
     2041 accagacaaa taatgccatg cagataaaac gttccagttg gttgcccttt tggggaacca
     2101 ggataggcaa gcggaaacgc cagcgacatg gccagagcag gggaacaccg gcgggtgtca
     2161 gcatatcggc aagtatgtgg cttaaataac ccagcaccat tccttgtagc gcatcagccg
     2221 gaatgaacca gctttcagga accttaaggt aaaaggttgc cagcagcgca aataccgcca
     2281 gcagactgtg ggtaaaacca cgatgcccaa aagcgcgggc gatcggtttt gatatccact
     2341 ttaatcgctg cccaagaaac gactttgggt gatcgatgtc cggtaacaaa cacgtcagga
     2401 ttgcggaagg gacaatatgc caccagtcac cctgtgccag cacgggcgtc agctcggcat
     2461 ttttggcaaa taccgcacaa gcaatagaaa agagaaggtg accttccgcc gtcatgataa
     2521 aactcactaa actgtcgata cgtacagtat aggattttta tacagtgcac ggaagtggtg
     2581 acggtgtaac tctttctctt aaattgacga ttaatccgtc agttgaatgc aaattcagat
     2641 aaagaaccag gggcagcgag atcactgccc ctggtcatta accgagaagg tcttttgcgg
     2701 tgagttctgt cagcgatgag agtttgacgt ttgctaatac aaaacgtgga tcattttgcg
     2761 cttctggcgc aggaacgaca atggaacgca tgcgggctgc tttagaggcg atcatgccat
     2821 ttactgaatc ttccagcgct acgcaggtca gtgggtcaac gcccagtttt gctgcgcagt
     2881 cgagatatac ttgcggatgc ggcttgctgt aaggcagttt ttcagccgag gcgagggcat
     2941 cgaaactgtc gcgtaagtca aacatggtca acactttttc cagcatatgt agtggtgacg
     3001 cggaggccag tcccaccaat aaaccttgtt ctttgcataa cgcaacggct tcgcgcacgc
     3061 ctggtaataa tggacgtgtc tcttcaacca gtgaaatggc acgggcaata acccgttcta
     3121 ctacttcctg acggcttggc ccattccacg gttgccgggc gtaccaaaga tcgaccacca
     3181 tatcgatgcg taaacctaag gtgtccggca gctcgttacg acgggagata tccaccccca
     3241 ggcttgccat cacatccagt tcggctcgat cccataaagg ttctgagtcg ataagtaatc
     3301 catccatatc aaaaattgca gcaagaatct gacgcggggt tgacattaca acctctcctt
     3361 tagagcatta tgtgtaaggt attaagccgt gcttatgatt aagcgcctat catatcgcct
     3421 ttaaagaatg ggcgaaaaca gatatctaca ggtaaactaa gtgaaacgac gcgtcttaat
     3481 caaggagaac tcatgacgta tcaacaagct ggacgcattg ctgttttgaa aaggattttg
     3541 ggttgggtga tttttatacc tgcattgatc tctacattga tttctttgct gaaatttatg
     3601 aatacccggc aggaaaacca ggaaggcatt aatgcggtca tgctcgactt tactcatgtc
     3661 atgatcgata tgatgcaggc gaatacgcct tttttgaatc tgttctggta taactccccg
     3721 acgcccaatt ttaatggtgg cgtgaacgtt atgttctggg tgattttcat cctgattttt
     3781 gtcggactgg cgttgcagga ttccggtgcc cggatgagtc gccaggcgcg cttcttgcgg
     3841 gaaggtgttg aagatcaact cattctggaa aaagccaaag gggaagaggg gctaacgcgt
     3901 gaacaaattg agtcccgtat tgttgttccg caccatacta ttttcctgca gtttttctca
     3961 ctatatatcc tgccggttat ttgtattgct gcaggttatg tgttcttttc tctgcttggg
     4021 tttatttaat caaaaggccg cacggagcgg ccattatcct caacccatat catgctgcta
     4081 ataatctatc caatgactgc tgagcaataa ccaaatgctg accgccaaat aatcttgcac
     4141 gatttaacag cgtgtagagt tggtaaaccg gttgacgttc aaggaaatcg gcaggtagcg
     4201 gggatactga ctgatagccg tcatagattt gtggcggctg ttcagtatgc agcggtaaca
     4261 tcgccaggtc gcactctcgg tcaccccagt agcaggcagg gtcgaaaatg tacgggccat
     4321 ccggacccag cgcacagttg ccggaccata aatcgccgtg caacagagaa ggctgcggtt
     4381 gatgcgaggc cagacgctgc tgaatatgct cgacgatggc gtcgatattg ccgaaagcga
     4441 tccctttctc tgctgccagt tccaactgcc agccaatccg ttgttcagca aaaaacgttg
     4501 accagcgacg ttgccaggtg ttgggctgtg gagttgtgga gagcgagtta tcaaaatcga
     4561 ggccaaattg tggttggtca ctccattgat gtaaacgcgc aatttgctga ccaagaataa
     4621 atgcgctatg cgcatccagc ggacgaggtg ggagataatc catcaccaga aaactgtagt
     4681 cacggtcagc gcctactgcc caaaccttag gcacggtgac ggttttacta cgcgacagta
     4741 actccagttg gtcggcttcg gcggtaaaac cgggaagcag ttccctttca tcacatttga
     4801 cgaaaaagtc atgtcctgca tagcgcaaat gccatgcggc atggacttct ccgccaggca
     4861 gttcattacg cagttcgatt tcgccttcac ctaactgctc gctcaaaaga cgactgattg
     4921 cctgccacat gatgtctctc ccatgttgtc tgccagatca taaggttagc gcaagaatgc
     4981 ggtgtaaaaa cacgagctaa cgcacaccac agactgatta ttgcccaaaa accatttatt
     5041 ttctgttgct attccattcc tcccaggtcg tgatggtgat ttcaggatct ttgccggttg
     5101 cactttgggt aagccccgaa accagttctc taatttcttc ttcactttgg gtactgataa
     5161 gtccaaaagt gttagtcccc agctcatgga tatttccctc atcatccgtc atggtgagca
     5221 aaaaaccgtc gcgagtcagg taattattca actcgttaat ttcagtcaat gtatcctcat
     5281 gcaacatgac ggttatgaca taacggacaa gatcgccact ggccatagtt cacccctttg
     5341 ttatcacatg acttttgagc atagtcggag aaacgcgttg ccgacaggtt ggtggtgatt
     5401 cccccaatgc tgggggaatg tttttgttag cgggaaaggt aagcgtaaat tttttgcgta
     5461 tcgtcatggg agcacagacg tgttccctga ttgagtgtgg ctgcactccc cgcagctacg
     5521 ccaaaacgga ccatctcttc aagagaggca ttttctgcca gtttcagtgt catcgcgccg
     5581 accatgctgt caccagcgcc aacggtactc tggcttttca ccggtggtgg caccacctga
     5641 atgcagtttt cactatcaac acccagcgct ccttgtggac caagggaaac gacaacccgt
     5701 ttggccttgc cgctattaac gatttcctgc gcggctttgc ggacatcgtc tggctgggtg
     5761 agttcgcgat tcaccagcgc actgagttct ttttggttag gcttaaccaa ctcgatgtta
     5821 ccaatcgcca gtgctgcact taacgcttcg ccagaactgt cgatgatgca gcggatccct
     5881 tggttttgcg cagcggaaat cagttgggtt aatttttcca gcttcacacc tggcggcagg
     5941 cttccgctta tgaccaggat ggccccggat tcaatttcca gaacttgctc ttcaagctgg
     6001 cgaaactcat cttcatttaa tgccgcgcct ggcataacaa aacgatactg ctcaccgctt
     6061 gcttccacat gtacgtgtaa attctgccgg gtccagtctt tggcttctac agtagcgacg
     6121 gggacatttt catccgccaa cagagaaacc aggtgttcgc cggtcgcgcc acccgccggg
     6181 aagatcgctg tggcactgcc tccaagatgg gcaatggcgc gggcgacgtt gatgccgccg
     6241 cccccgggtt cgaacaccgg tgcggtacag cgcagttttc cttcgggata aatttgcggg
     6301 gtaattgttg cgctatcgag agagggcgca agtgtcaacg tatagatacg taccatcatt
     6361 tcctcctata ggctgatttc agtctggcac cgaatcagcc gaaagtgaag aaattaacaa
     6421 tatgatttat taga
//
LOCUS       DAZGJW010000215         6357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZGJW010000215 DAZGJW010000000
VERSION     DAZGJW010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.216"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            682..816
                     /locus_tag="ACHKIK_004653"
     CDS             682..816
                     /locus_tag="ACHKIK_004653"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085672.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            1417..2238
                     /locus_tag="ACHKIK_004654"
     CDS             1417..2238
                     /locus_tag="ACHKIK_004654"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO9085673.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            2238..3344
                     /locus_tag="ACHKIK_004655"
     CDS             2238..3344
                     /locus_tag="ACHKIK_004655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO9085674.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            3438..5159
                     /gene="cptA"
                     /locus_tag="ACHKIK_004656"
     CDS             3438..5159
                     /gene="cptA"
                     /locus_tag="ACHKIK_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO9085675.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            5233..6231
                     /gene="lpxM"
                     /locus_tag="ACHKIK_004657"
                     /gene_synonym="msbB"
     CDS             5233..6231
                     /gene="lpxM"
                     /locus_tag="ACHKIK_004657"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO9085676.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLIAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
BASE COUNT         1674 a         1358 c         1553 g         1772 t
ORIGIN      
        1 atacccctaa ttcaactaat tggaggcatt accatgcatt agtgcaagcg ccttgtggat
       61 tcttttgcta atggcggaaa catattgtac aatattgcat ttatccctca ataaataaat
      121 gagttatcag attgcaaaaa tctgttatca aactaattca aataaaaaga gattgatacg
      181 accaacgtac acaaaaattt tcgatattcg aactaaataa cagctcctaa taccgctaca
      241 ctttagctat ccagtctgcg cctcagaaat ggacgatgcc gttgaaggac tggtgtatgg
      301 caatgagccg ctttattatc gaattcggtg accgcttgga cggtcacttc ggagaaaagg
      361 catttacaca gcatcctaaa caggttcagc ataggtgata taccgccttt tcattttctt
      421 ctccattatt tataagggag gtgtattatt atgcaatgtt attggtttta tccattgttt
      481 tttagtttat gaatgttcgc aaaaaaataa atgaacaata attttatgta tttaaaataa
      541 attaatttta cttctgtaat ggtgtcatga ttagcagctt tttaaactaa tatcgacttt
      601 caataaataa ggacaggtaa tggacaacaa aaatatctga acccgggtga gattcataag
      661 ttgcacagtg ctgtcttcaa agtgccacac ccggaacgta atcactgcct gctccttatg
      721 gggtatcttc atggtgtaca agcatcagag ttattaggta taaaactgtc tgatattgat
      781 ttgcaggccg gtaacctgaa tatccggcgg ctctaaacgg tttgtcaact cagcccctcg
      841 gctgaaacag gaggtacagt ccattcgtcg ctggctgaaa cttcgggatg aatgggtaga
      901 agagggatat gaatggttat ttaacctcta tggcaggtct gctacaggta ttactgttgt
      961 tctggggggc actggtgtgg ttaatatcta cgatgcaaga gattctcacg atggcccggt
     1021 ccatcagtgc atgcggcgcg atattgtgcg agtatttttc agggggagtg gtatccggat
     1081 gcagaaaatt ccgctactcg actggtttta tgcaataaat caggatgata atcttctgat
     1141 tttttactga ggcggatatc tctcgctgtg tttactgaaa ttgcggtatt ctgttttttc
     1201 tgaaaaacag aaaatctgat taaaaagagc atatgcgtta agggaaattt aagttctggt
     1261 tgattaactg ataaaaatat taaaaaatcc ctgttgtcgg taatctccgg accattccgg
     1321 gattacacaa taatatcacc atgtgtgata tctatggatc ctcactgttg tttacgcgag
     1381 gatattttca gaatgcttaa cagacgataa ttttttatgt tgaatgcaag gcatcttccc
     1441 gtactgatgt atcatcatgt cagtcgttgt ccggggcttg tgacactctc acctgaaaca
     1501 ttttgtgaac agatgaagtg gctcgcagat aataactggc ggacggtaac atctgctgag
     1561 cttgaatatt tttatcaggg gggaacgctt ccacgaaaga gtgtcatgct cacattcgat
     1621 gatggttatc ttgataactg gctcagagca tggccggtcc tgaaggaata taaccttcat
     1681 gcacatattt ttctcattac ggggctgatt ggtaagggaa acgtacgtag caggcaggag
     1741 caggaatatt ctcaccgcga ctgtgaacgg ttgattgccg ataaccgttc agatgaggtc
     1801 atgcttcgct ggtctgaagt ccgggaaatg cgtgattccg gactggttga atttcatgtg
     1861 cacacccatt cacataaacg ctgggacagg ttgtctgtat cacgtgctga acagtgccgg
     1921 ctcatgaaag aagatattct ggtgggaaaa caatgtctga cggaaaagct ggggttctgt
     1981 agttcccatc tgtgctggcc tgaaggatat tataatcggg attatattaa tctcgccggt
     2041 aagcttggtt tctcttattt atatacaacc gaaagaagaa tgaactgtcc tgaaaatggt
     2101 tcattacgca tagggcgtat cagcaccaaa gagcgggaac attccggctg gctgaaacga
     2161 cgcctttttt attatacaac gccacttttt tcttcagtac tggctcttca taaggggcca
     2221 agattaccag ataattaatg atgaaaattt tattcactga atcttcgtca gatattgggg
     2281 gccaggagct gcaggcactg gcccagatga ctgctcttca aaagcaggga cattccgttt
     2341 tacttgcctg cagggaaaaa agtaaaattg ctccggaagc cagaaaaaga gggcatgatg
     2401 tcacttttat tcctttcagg aacagccttc accttccttc catcctcaga cttcgacgga
     2461 ttatcgggga atttaaacct gatctggtca tctgccatag tggccatgac agtaatatag
     2521 caggactgag ccgtctgata tgctgtcacc gcttcagtat tgtcagacaa aaaacctaca
     2581 tcacccgtaa gacccgaacg ttttccctga attatctgtg tgatttcatt gttgtgccta
     2641 gctcagccat gatggcgcat ctgatggcgg aaggtgtcag gacaccggta acggttattc
     2701 caccgggttt cgactggccc gcattgcata atgaagccat gcgaccgtta cccctgcata
     2761 tccacgcctg ggctgcatca gcagacaatg ttccgcttat cgttcaggtg gggatgctac
     2821 gtcccgaaaa aggacatgaa tttatgctgc gtgtattgta tcagctgaag atggaaggga
     2881 aatcatttcg ctggctcgtg gtgggcgccg ggcgtgaaga atacgaagca cgtctgcgtc
     2941 agcagacaga acacctggga atgagcggag atgtgttaat ggcaggtgcc cttttcccag
     3001 cgttaccggt ctaccggatt gccagtgtgg tggtcatgcc ttcggaaaat gaagcgtttg
     3061 gtatggtact ggctgaagcg tcagtctctg gcgtgcccgt tatcgccagt gagacgggcg
     3121 ggatccctga tgtcattcag aagaatgtga cagggacgct gttacccgtg ggggatgtgt
     3181 ctgcatggac cggcgcactg cgcgattttt tatcccgacc ggaacgcttc cggatgatgg
     3241 cagccagcgc cagagaagat attgagtacc ggtttgatat taacagaacg gcacagatca
     3301 ttgtgtctct ggctagtcag gctaagggga aatgtaacag ataacggcgg atcctctttt
     3361 cagcccgaat tttgttcagc ggttttccgg caagtaacgg agtaatttat cacattgttt
     3421 aaatacgggg acggtcaatg catttaaata ccggacagaa caggcctact ttcagctgga
     3481 gtgcgctggg atgggcaatt ttttattttg gctttttttc cactctcctg caggtcatca
     3541 tcttcagcag tgggtacagc ggaacgaatg gaatacggga ctcactgtta ttcagttgtc
     3601 tgtggttgat cccggtgttt ctctatcctg atcggataaa aataattgca gctgttgtcg
     3661 gtttcattct ctggggcacg tcgctggcag cactttgtta ttattttctc tatggtcatg
     3721 aattctctca aagtgttctt ttcgttatgt ttgaaacgaa tgccagagag gctggtgaat
     3781 atttcagcca gtatttcagc cttaaactgt tgcttatctc gctggtatat actgcggtgt
     3841 ccgtttttct gtggacacgt ctgcgtcctg tatatattcc tttgccatgg cggagaattg
     3901 tctctttcct gctgctttat gctctgcttc tgcatccggt tgttctgaaa tcgttaatca
     3961 gacaggagcc gctgaatgat actctaggca aactggcatc ccgaatggag cctgctgccc
     4021 cctggcagtt tgtatccagt tattatcagt accatcagca actgaatgca ctgacaacct
     4081 tcctgaatga aaatagcgca ctgccaccac tgggtaatct cagggatgaa tcaggggaga
     4141 gaccacgcac actggtcctg gtgattggtg agtcgacaca gcgcgaacgc atgagcttat
     4201 acgggtatct acgtgaaacg acgccggagc tggatgcact gcgtaaaacc gatccgggtc
     4261 ttactgtgtt taataatgtg gtggcatcgc gtccgtacac cattgaagca ttgcaacagg
     4321 cccttacttt cgccaacgaa aagaatcctg atctgtatct gacgcagccg tcgctgatga
     4381 acatgatgaa gcaggcaggc tataaaacat tctggattac caaccagcag acaatcacag
     4441 cccgtaacac catgctcact gtattttcgc gccagacgga caggcagtac tacatgaatc
     4501 agcagcgaac acaaagtgcg cgtgaatatg acactaacgt gctgaagccg ttccgggaag
     4561 tgctgaatga ccctgcacca aagaaactga tcatcgtgca tctgctgggt acgcacatta
     4621 agtataaata ccgttatccg gaaggtcagg ggcggtttga tggcattaca gggcatattc
     4681 ccactggatt aaatgcgaaa gagctggaag tgtataacga ttacgataat gccaatctgt
     4741 ttaacgatca tgtggtggcc agtctgataa aggacttcag agcaactgcg ccggacggct
     4801 ttctgcttta tttttcagat catggtgaag aagtatatga tactccgcca tataaaacgc
     4861 agggacggaa tgaagataac cccacacgtc ccatgtacac tgttccgttc ctgctgtgga
     4921 cctcggaaaa gtggcatgct gcacatccgc gagatttttc gcagtatgtt gaccgcaaat
     4981 acagtctggc tgaactgatc cacacctggt cagatttggc gggactgaca tacgatggtt
     5041 acgatccgac ccgttctctg gtgaatccgc aattcaggga aactacccgc tggattggaa
     5101 atccgtataa aaagaatggg ctcactgatt tcgacactct tccgtatggt gagccatagt
     5161 attttcttat cttcggttcc gttctgaagg caggaggatg tgttttcctc tgctgtccgg
     5221 tggactccag ggatggtacc accttctgct gtactctgtt atcataatga gataagcagg
     5281 cagataccgg taaatatgaa aaatatcaga actgagttta ttccccgctt taaccttaca
     5341 ttatgcttcc cccgttactg gatgacgtgg acaggtatcg gtattatctg tgtgtttgca
     5401 atggtccccc ccgcactgag agatccattg ttgggaaaat tgggcatgct ggttgggcgt
     5461 ctggggaaaa gcgcacgtca acgggccttg attaatttat cgctttgttt tccggagtac
     5521 agtgataaag aaaaggagaa tatcgttgat gccatgtttg caacagcctc catggccgtt
     5581 gtgcttatgg ctgaattagc actgtccggg cctgataaaa tttcgcatcg tatacgctgg
     5641 aatggtctgg agattgtgga gaagatggca cagaacaatg aaaaggtcat ttttcttgtg
     5701 ccacatgcct ggggagtgga tattcccgca atgctgatag ctgcgtcggg cagaaaaatg
     5761 gcagcgatgt ttcataacca gagaaatccg gtggtggatt atgtctggaa cagtgttcgt
     5821 cgtcgtttcg gaggaaaact gcacgcacgt aatgacggta ttgcctcctt tgtccgctca
     5881 gtccggcagg gatactgggg atattatctt cctgatcagg atcatggccc ggaattcagt
     5941 gagtttgcag atttttttgc gacgtataaa gcaactttac cggttatcgg acggctcagc
     6001 aggatcagtg gtgcacggat aataccgctt ttccctgttt atgacggaaa gacccatcat
     6061 ctgactattc atgtcagccc gccacttgct atacgacaga agagtgatgc tcatattgcg
     6121 cgacagataa acgaagtggt tgaaaatttc gttcgtccac atcctgagca gtatacgtgg
     6181 atcctcaagt tgctgaaaac gcgtaaagaa ggggaagagg atccttactg aggcaggttt
     6241 ttcaggataa tgagcttacc cagcaatagt ggacacacgc taagtgagta aactctcagt
     6301 cagaggtggc tcatatgaca aaaaaacagt accaaccagt aaaaaacccc gtaaata
//
LOCUS       DAZGJW010000216         6239 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZGJW010000216 DAZGJW010000000
VERSION     DAZGJW010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.217"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..272)
                     /locus_tag="ACHKIK_004658"
     CDS             complement(<1..272)
                     /locus_tag="ACHKIK_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFO9085677.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRV"
     gene            complement(269..1939)
                     /gene="menD"
                     /locus_tag="ACHKIK_004659"
     CDS             complement(269..1939)
                     /gene="menD"
                     /locus_tag="ACHKIK_004659"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO9085678.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(2028..3323)
                     /gene="menF"
                     /locus_tag="ACHKIK_004660"
     CDS             complement(2028..3323)
                     /gene="menF"
                     /locus_tag="ACHKIK_004660"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO9085679.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3402..3707)
                     /gene="elaB"
                     /locus_tag="ACHKIK_004661"
     CDS             complement(3402..3707)
                     /gene="elaB"
                     /locus_tag="ACHKIK_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO9085680.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3762..4223)
                     /locus_tag="ACHKIK_004662"
     CDS             complement(3762..4223)
                     /locus_tag="ACHKIK_004662"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9085681.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            4288..5205
                     /gene="rbn"
                     /locus_tag="ACHKIK_004663"
     CDS             4288..5205
                     /gene="rbn"
                     /locus_tag="ACHKIK_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO9085682.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            5393..>6239
                     /locus_tag="ACHKIK_004664"
     CDS             5393..>6239
                     /locus_tag="ACHKIK_004664"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HFO9085683.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDDT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFHINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKENSLKRVI
                     NSFNSE"
BASE COUNT         1569 a         1668 c         1478 g         1524 t
ORIGIN      
        1 acccgtccgc caagcgagta ccccaccagc cagaagtcaa ggatgttgta actaaccaag
       61 gttttacgca gtaagtcggt gacatcatca aatccatcga cgctaatcgc cgccgaacca
      121 ccatgacctg ggagatcaac atacaaccgt gagtagtcgg caaacgcctc gcccacttct
      181 tgccattcgt ggcaatcgcc ggaaaaaccg tgcaaaaaca ccagccaggg taaacctggt
      241 tttccgtgtt ttgcctgcgc gtgcaggatc ataaatggct tacctgcgcc agaagttgct
      301 ggagcgtttg cgcaccatcg gtgtcgttaa ccaccatttc aatcaccgtg gtggttggcg
      361 tgcgccaggc gtcggcaaat gccgtttcaa gttcctgcca gttttgcgga cgatgatatt
      421 tcagctcgaa catcgcggcg gcgtgctcaa aatggacgtt ttgcggcatc agatagaaac
      481 gctcacgctc gctttgcggc gttggcaaca gcgagaaaat ttgcccgccg ttgttgttca
      541 ccacaattaa taccagcggc gcagaaacct gacgcaataa cgccagcgcg ttgagatcgt
      601 aaagtgcgga gagatcgccc acaatcgcca gcgtcggttt gccgcttgcc cgctgaacgc
      661 ctgcggcggt cgaaagcagt ccgtcgatac cgctggcccc tcggttgctg tacaccgggt
      721 aacctgccgg aagttgcgaa agcgcatcaa tcagacgtac caccaggctg ttaccaacaa
      781 acaattgccc ctgttcaggc agatagtcgc tgatgcgatg cgccagttgc gcttcgccaa
      841 acgcatcacg gcgggcaata accgcctgca ttgcctgttc cgccaggcgc gggatttcaa
      901 cgcaccaggg ctggcgtttt tctgccggat gcagctccag ccagtcggca atattggcaa
      961 ttaagcgacg tccgcgatgg tgtgccggat caagtcgccc ttcaatgtca tcaacaatcc
     1021 agtactcttc gggttcacag cttgcctgcc attgcaggag ccgtttcccc gtcaggctgc
     1081 ttcccagttg caccacaatt tgcgcctgct gcagctcgct ggtcgctttg gcattgccta
     1141 accaaagatc ggcacacggc agcggctgcc cggtttgtga cagcacatcg ccaatcagcg
     1201 gccagccaag agtttgcgcc cacagggcaa cttttttgcc ctcttccgca ctcatgcgcc
     1261 cggcaaccac cacgccgcgc ttttgtcgcc agaagaacca gtcgcgctgt ttttcacttt
     1321 ccagacgagg cgcttcacgc agccacggtt tgtcgtcctg ccaccagtca cccagacgct
     1381 gttgccagct aagcccggta tcgtccattt cgccatacag cggttcagca aacgggcagt
     1441 tgatatggac tccccccgca tgaagcgtac cgagagcgtg gtcgatggta gaaaccagcc
     1501 aacgtgcggg gatatcctgg gtcgggcgcg gcaatgaaat actgtgcgtg gggtgagagg
     1561 cgaacattcc cggctggcga attgcctgat tcgcgccgca gtcaattagc tccggcgggc
     1621 gatcggcggt taagagaatc agtttttctc cggttaaccc ggcttcaatc agtgccggat
     1681 agagatttgc caccgccgtg ccggaggtca caatcaccgc caccggctgc ttgctgactt
     1741 tcgccagccc cagcgccaga tgccccagtc cacgctcatc gaaatgggtg tggtgaatga
     1801 atgcggaatt ctccgccgcc gctaacgtta acggtgtaga acgcgagcct ggggcgatac
     1861 agatgtgtct gacgccgtga cgcgttaatg cttccagaat gaccgccgcc cagcgtcggt
     1921 taaatgcgct tactgacatg agtttgtccg gtatcaatat tgcggctaag tataaggagc
     1981 tacaaaaata gattattgat atgaatcggt aatgatgcga ctcattacta ttccatttgt
     2041 aataaagtac gcagccctgc cgctttgttg tcgatttcct gccactcttg ctcggggtcg
     2101 gaaccacgga caatgcccgc gccagcatat agccgcacga cattgccgct gatttttgct
     2161 gaacgcaggg aaacgcagaa ttcgctttgt tgtaatgaga gatagcccgc agaaccggcg
     2221 taccattctc gggtgaacgg ttcgtgacgg gcgataaact gtcgcgccag atcgcgcggt
     2281 aagccagcaa ctgctgccgt tggctgcaac tgatgtaaac agatcacatc atccgctttg
     2341 ttgagtgaag tccagataca gcggcgaaga tgctgcactt tacgcagacg cagtacctgc
     2401 ggcggtaaaa catccagcgt ctgggtatcg gcctgtaatc gttggcagat atcttccacc
     2461 accagcatgt tctcgcgctg gtttttatca tccgccatca gccactctcc taactgctgc
     2521 gcctgcttat catcaggatt atttgctact gttcccgcca gcgcttcagt acgcagcgct
     2581 ttgtcacgcc gccgccataa ccgttccggt gaagagccaa gaaaagcatt ttcgccatca
     2641 aaggccatgt aaaaatggta gcaattcaga ttcagtcgac gactggcagc catcatcgcc
     2701 gccgcgttga ccggacttgc gaaatgcagg tcagttgccc gagcgagcac cactttgtcg
     2761 agctcacctt cggcgatggt tttcgttgcc agttcgatta attgcgtcca gcccgttttg
     2821 tccggccagt gttgttctcg cgtggtggtt aaatgtaacc caggcaaggg cttgatactc
     2881 accagtgtgg cgataaattc ttttgcctga atcgcatcgt gctgaaggga gctttcgctg
     2941 aatagcgtca gccgcagcgt ggctttaccg ccacagcgtc gccattccag gcggggtaaa
     3001 agtaaattgc cctgcgacgg gtcaaaagca ttcagccccc aaatgcgtaa gtcggcgtgt
     3061 tccgggtgct ggcgaagaaa acgttgtgcc tggtccaacg acgtaaaacg ggtaatcgcg
     3121 cccaggacgg ctgcttcttc atcaccatta cgttgttgcc agtagaattg cgggtacgtt
     3181 tgctgactgg ccagccagct caaggcatca aaagcgtctt tgagagggaa aggaatatcg
     3241 ataacccgaa tgccgggtgt cgccggaatc tcttgcgaca aatggcgcag tagattttcc
     3301 agcgccgtag taagtgattg cacgcggacc tctccctgtt aaaagcctca cattatacgg
     3361 ggtactacaa aaaaaatgca gtaccccggt gtagggaggt tttaacggcg tgccagcaac
     3421 agtcctagta ccagcccaac ggccgcgccc acaccaattc cttgccaggg tttttcgtgg
     3481 acgtagtcat cagcacgata aactgcctgc ttcgcccgat agtaataact gtctgaagcc
     3541 tggctaaccc gttttttcac atcatccagc gctttttctg cacgcgcttt cagctcaaca
     3601 tatttctgat cggcgggatc gccagaggag cggagcacct cttccagtgt ttcactaagc
     3661 agcgtcaggt cgtcatcgat acgtgtatca ccaaactgat tagacattct cgttctccat
     3721 ttgcgtaaaa cctgtcacta actatagaca agggttgttg attacgcctg aattacctcg
     3781 cgtgccatcc caatgtgtgg gataccatct tcttcataga cctccgtcac agggataaaa
     3841 ccgaaactct ggtagaagtt ttgcagatgc gcctgcgccc cgaggtaaac aggcttatca
     3901 ggccagtgat gcgtacatgt ttcgagtgtt ttactcatca gctgctgccc cactttttcg
     3961 ccacgcaacg cttcactgac aatcacccga cctataacga ccggctcaag atcatcatca
     4021 cttttcagaa tcctcgcata tgccaccagt tcatcatttt tccaaccgag aatatggcga
     4081 ttatcccccg tcaaatcatc accatcgata tcctgataag ggcaattctg ctcgacaaca
     4141 aacaccgcgc aacgcagttg taataaggca taaagctggg aaaccgataa ttcagagtga
     4201 tgcagatctt gccattcaat cataatgctc tccttttaga tgtatgcctt atactagccg
     4261 gaatattttt tgaaacgtga tgaactcatg gaattaattt ttttaggtac ttcggccggt
     4321 gtgccaaccc gcacgcgcaa tgtcacggca atattgctta acctgcaaca tccgacccag
     4381 agcggtcttt ggttgtttga ctgcggtgaa ggcacccagc atcagctact gcataccgcc
     4441 tttaaccctg gaaaactgga caagattttt atcagtcacc ttcatggcga tcatcttttt
     4501 ggtttacccg gcttgctgtg cagtcgttct atgtcaggca ttatccaacc cttaacgatt
     4561 tatggtccgc aaggtatccg tgaatttgtg gaaaccgcgc tgcggattag cggctcatgg
     4621 accgattatc cgctggaaat tgtcgaaatt ggcgctggcg aaattctcga tgatggcctg
     4681 cgcaaagtaa ccgcttatcc gctggaacac ccactggaat gttatggcta tcgtattgaa
     4741 gaacatgata aaccgggtgc attaaatgcc caggcattaa aagctgctgg cgtgccgcct
     4801 ggcccactgt ttcaggaatt aaaagcgggc aaaacaatca cgctggaaga tggaaggcag
     4861 attaacggcg cagattactt agctgctcca gtgccaggta aagcgctcgc tattttcggc
     4921 gataccggcc cctgcgatgc cgcacttgac ctggctaaag gtgtcgatgt catggtgcac
     4981 gaagcgacgc tggatataac catggaagcc aaagccaata gtcgcggcca tagctctaca
     5041 cgccaggctg cgacactagc ccgtgaggct ggagtcggta agctaatcat tacccacgtc
     5101 agctcgcgct atgatgacaa aggttgtcag cacctgttac gtgaatgcag gtcaattttc
     5161 ccggcgactg aactagcgaa tgatttcacc gtgtttaacg tttaactctg cttattatta
     5221 aaaacagggc gaaacttgcc ctgttatcgc aacccgcgct acatatatcc acccacacgg
     5281 gcatattgag cgatttttga tatttattca tctccttacg cactatattc agagcaataa
     5341 gttgcctctt tttatcttca ttcagcacaa cctttgcaac taaaaggagc ccatgatggt
     5401 tacagttgtc agcaattatt gtcaattatc tcaaacccaa ctcagtcaga catttgcaga
     5461 aaaatttact gtgaccgagg aattactgca gtctttaaaa aaaacagcgt tatccggaga
     5521 tgaagaaagt attgagttac tgcataatat tgcgttaggt tatgatgaat ttggaaaaaa
     5581 agctgaagat attctttacc atattgttag aaacccaaca aatgagaccc tatcgattat
     5641 ccgacttatt aaaaatgcct gtttaaaatt atataatttg gcacataccg caaccaactc
     5701 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
     5761 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
     5821 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatgata caaacttctg
     5881 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
     5941 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
     6001 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
     6061 agtccagtta ttccacataa atactggcgg acactggata ttagtttcgc tacagaaaat
     6121 agtaaataaa aaaaataata aactacaaat aaaatgcgtc atattcaact cattgcgtgc
     6181 actaggccat gataaagaaa attcacttaa gcgtgtcatt aatagtttta attctgaac
//
LOCUS       DAZGJW010000217         6184 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZGJW010000217 DAZGJW010000000
VERSION     DAZGJW010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.218"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(385..1512)
                     /locus_tag="ACHKIK_004665"
     CDS             complement(385..1512)
                     /locus_tag="ACHKIK_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9085684.1"
                     /translation="MAAVILRKVEKIYDNGFKAVHGIDLDINDGEFMVFVGPSGCAKS
                     TTLRMIAGLEDVSGGEIYISDRLVNTLPPKDRSIAMVFQNYALYPHKTVFDNMAFGLK
                     MQKRPKEEIKKIVEEVAEKLEISDLLYRKPKEMSGGQRQRVAVGRAIVRKPDVFLFDE
                     PLSNLDAKLRVSMRVKIAQLHQSLKDEGHPATMIYVTHDQIEALTLGDRICVLNQGRI
                     MQVDTPVNLYNHPANRFVAGFIGSPSMNLIDAVVREEDGKLYIDIAKNIRLYIPENKQ
                     SVLKAYIDKPVCFGIRPEHISLSLNCKEMNTVTGALSIIENMGSEKYLYFTVNNKEFI
                     AKVNDQSITTADIGKNLYFNLYTSFCHIFDFYTEENLTNYL"
     gene            complement(1595..2083)
                     /locus_tag="ACHKIK_004666"
     CDS             complement(1595..2083)
                     /locus_tag="ACHKIK_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HFO9085685.1"
                     /translation="MKNKLLIAAVLTGLLSGCVDGNSPSGSQEGVVKADPRYSECNLP
                     DVNERGPIAAGLYISGTLPEGYWLMKPERKMHYKGDGIYQLIYSEKAGAVTMQFATMN
                     WNPQFTVTGLELPLNQAKTLKKGGMDKNTKVVIPADGKYVWTIQIGPDKKAIAAMVAP
                     CK"
     gene            complement(2174..3130)
                     /locus_tag="ACHKIK_004667"
     CDS             complement(2174..3130)
                     /locus_tag="ACHKIK_004667"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO9085686.1"
                     /translation="MFSALDIKTLMQGTLYGDPSLRVDTIRPIHSPLVGGLSIVMTPG
                     DLLHIPTTGADIIIGPEEIISSNAKAKIVVDYLNVNNLNKVLRYFKVHKYRLFEQENT
                     STIPDVYIGKHCQIGMNCHFMPGVKIMNCVTIGDNVAIHANTVIKEGTIIGNDVIIDS
                     NNSIGNYSFEYMSDERDSYVRVDSIGRVIIGDDVEIGCNNTIDRGTLGDTIIGQGTRI
                     DNQVQIGHDCIIGNKCLIVSQCGFSGHVVLGDHVITHGQVGIAGHISIGSYSVIKAKS
                     GVSHSCPEKSDLFGYPAKNTREYNKNLAVLNKLTKQHGVYKQ"
     gene            complement(3149..4186)
                     /locus_tag="ACHKIK_004668"
     CDS             complement(3149..4186)
                     /locus_tag="ACHKIK_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO9085687.1"
                     /translation="MVLIKKNVLSASIATVLLGSTGAFAANDRGFTSDTDWSEEQAGF
                     NGHVGTTFEYETKSTDNYLGKTVKEYTKTHEIFQAYFSQPNWQFAAIYGLKSIDRRQE
                     EKGYHENETSLQNYISLNKTFTIGNGFDFSLVYDLMYTDGNIDSPYVTGLHTVTVDNS
                     FRPTLTYFNSDWNAGFYSNVEYLYSRNDKSSWGVREEEGQSILFQPYKRFGAWEFGIE
                     FFYQKKHNDEFNHGKINDRSIYTETYYEPIIKYAFENAGNLYLRTRFGHGKTENADSL
                     QYNANRTYFKTIRKATLGYEQKIGDDWIAKIEYKHAWEKEHKNFYDPRDEEKYNVLNQ
                     DNIYVHAFYRF"
     gene            4459..5691
                     /locus_tag="ACHKIK_004669"
     CDS             4459..5691
                     /locus_tag="ACHKIK_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9085688.1"
                     /translation="MRLSNIVTVIILAISSTLTPQAMAEKLIPETDAELLIWSDATSA
                     EYMKYAATEFNKDFGYKVKFTFRNIAPMDAASRIMQDGGTTRVADVAEIEHDTLGRLV
                     VAGGVMENMVSAERIKKTFIPGAVSAATYNNISYGFPVSFATLALFYNKDLLNTAPKT
                     FEEINTFSEKFNNSSEHKYALLWDVQNYYVSRMFITLYGANEFGKTGNDPKALGIASS
                     EAKKGLETMKRLKKANPSNPLDMGNPQVLRGLFNEGKVAAVIDGPWSIQGYIDSGINF
                     GVTRIPTLDGHQPRTFSTVRLAVVSSFTEYPHAAELFADYLTTDKMLMKRYEMTNLIP
                     PIDSLMNKISQTGSEAIKAIIAQANYSDAMPSIPEMSYLWSPMTNAILATWVENKTPD
                     EVLNHAQTIIEEQLSLQE"
     gene            5694..>6184
                     /locus_tag="ACHKIK_004670"
     CDS             5694..>6184
                     /locus_tag="ACHKIK_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFO9085689.1"
                     /translation="MNTQQLKMKSAPVLPISCLIMGGTQLSRHYYVKGGIFFAIQVCF
                     LLYLSDIVHTLIGLFTLGDVAQIRKGLTVIQGDNSIFMLVEGVIATIIVGLFATIYIL
                     NIKDARNSSYCHLTFKQQLYKLYEDKFAFIVLTPAFLASIAFIVLPIVITVLVSFTNY
                     AAP"
BASE COUNT         1841 a         1220 c         1141 g         1982 t
ORIGIN      
        1 tgaaatctta ccaattttaa cattattaat taacattttc gaaaaaattg ctgagctaaa
       61 atgtggattt atcgtaattc atcctcaagc tggttacagg tctacagtac gtaagcattg
      121 tgaactgcga atctgaatgc cctccaacta tggtcagaag acatgctctg acctaaatag
      181 taggctcact gagtaagcgg tgttatcaac ggtttattca tatatgctct tttttatgga
      241 tggaattttg taactcaatt accttgataa gaatcagttc gtggagttaa tgacatttac
      301 caatagacag ttcgagcagt aagaagcctt atggaattgt aaggtttaat accgttattc
      361 aacctggggt tattgtcata tcaatcacaa ataattagtt agattttctt cagtgtaaaa
      421 atcaaatata tgacagaagc ttgtataaag gttgaaatac aaatttttac caatatcggc
      481 tgtagtaatt gattgatcgt tgacttttgc aataaactct ttgttgttaa cggtaaagta
      541 aagatatttt tcactgccca tattttcaat aatacttaat gcaccagtga ctgtattcat
      601 ttctttgcaa ttcaaactca aagagatatg ttctggacga ataccaaaac aaacgggttt
      661 atctatatac gctttgagta ctgattgttt attctcaggt atgtacaacc gaatattttt
      721 ggcaatgtca atatagagtt taccgtcttc ctcacgtaca accgcatcta tcaaattcat
      781 tgacggggaa ccaataaagc cagcaacgaa tctgttagca gggtggttat acagattaac
      841 tggtgtgtcg acctgcatta ttcgcccctg attaagcaca caaatacgat caccgagggt
      901 caatgcttca atttgatcgt gtgtaacata aatcatggtt gctggatgac cctcatcctt
      961 gagagactga tgcaactgag caattttgac tcgcattgaa acacgaagtt ttgcatcaag
     1021 gttagatagt ggctcatcaa ataagaaaac atctggctta cggacaattg cacggcctac
     1081 cgcgacacgc tgacgctgac cacccgacat ttctttcggt tttcggtaaa gtaaatcgct
     1141 aatttcaagt ttttctgcaa cttcttcgac aattttctta atctcttctt ttggcctttt
     1201 ttgcattttt aaaccaaaag ccatattgtc aaatacagtc ttatggggat acagcgcata
     1261 gttctggaac accattgcaa tgctgcgatc tttaggcgga agggtattaa caaggcggtc
     1321 gctaatgtaa atttcaccac ctgatacgtc ttcaagacct gcaatcatgc gtaatgtagt
     1381 tgatttggca cacccagatg gtccaacaaa aaccatgaat tcaccatcgt ttatatcgag
     1441 atcaatgccg tgaacagctt tgaatccatt gtcataaatt ttttcaactt tgcgtagaat
     1501 taccgcggcc ataatctgct ctcattaaca agaattttgg aatactgata ctccactatt
     1561 caacgaatgt tgaatagtgg agtgctaaaa ataactattt acatggtgct accatggcgg
     1621 ctattgcctt tttatcagga ccaatttgaa tcgtccaaac atactttcca tcggcaggaa
     1681 taacgacttt tgtattttta tccatgcctc ctttcttgag tgttttagcc tgatttaatg
     1741 gcaattcaag acctgttaca gtgaactgcg ggttccagtt catagtcgca aattgcattg
     1801 ttaccgcacc tgctttttca gaataaataa gctgataaat accatctccc ttataatgca
     1861 ttttacgctc tggcttcatt aaccaatagc cttccgggag ggttccggaa atgtaaagac
     1921 ccgcagcaat tggccctctt tcatttacat cagggaggtt acattctgaa tagcgaggat
     1981 ccgctttcac gacaccttcc tgactgccag agggtgaatt gccatcaacg catcctgaaa
     2041 gaagaccagt caacacagcc gcaataagca atttattttt catttaaact ctcaataaaa
     2101 gtagtatagc ttagttaagc tatttctatg tcagtgttaa tgttaatgta tataaataat
     2161 acttctcaac gactcactgt ttatatacac catgctgttt agttagttta ttaaggacag
     2221 cgagattttt gttgtattct cgcgtatttt tggcagggta accaaaaagg tcactctttt
     2281 caggacaaga atggcttaca cctgatttag ccttaattac cgaataacta ccaattgata
     2341 tgtgaccggc gattccgact tgaccatgtg taataacatg gtcgcctaaa actacgtgtc
     2401 ctgaaaaacc gcattgtgaa acaatcaggc atttattacc gattatgcag tcatgtccaa
     2461 tctgcacttg attatcaata cgagtgcctt ggcctataat cgtatctcca agtgtccctc
     2521 ggtcaatggt gttgttacaa ccgatttcaa catcatcacc tatgattact ctaccgatgc
     2581 tatcaactcg gacatatgaa tcacgttcat ccgacatata ttcaaaacta taattaccga
     2641 tggagttatt agaatctatg ataacgtcat tgccaataat agtaccttct ttaatcactg
     2701 tattcgcatg aatggcaaca ttatcaccta tggtcacaca gttcataatt ttaacaccag
     2761 gcataaaatg gcagttcata cctatctggc agtgtttacc aatgtataca tcaggtatcg
     2821 ttgaagtgtt ttcttgctca aacaaacgat acttatgtac cttaaaatat cttaaaactt
     2881 tgttaagatt attcacgttc aggtaatcga ctacaatctt tgccttagca ttagaactaa
     2941 tgatctcttc cggtccgata attatatccg caccagtcgt tggtatatgc aacaaatctc
     3001 caggtgtcat aacaatcgag agtccaccaa ctaaaggact atggattggc cgaatggtat
     3061 caaccctgag tgatggatca ccatataaag ttccttgcat caacgtttta atatctagtg
     3121 cgctgaacat aggtttccct ctgtttaatc aaaagcgata gaaagcatga acataaatat
     3181 tatcctgatt gagcacatta tatttttctt catctcttgg atcataaaag tttttgtgct
     3241 ctttttccca ggcatgttta tattcaatct tggctatcca gtcgtcacct attttttgtt
     3301 cataacctag agtggctttt cgaattgttt tgaaataggt tctgtttgca ttgtattgca
     3361 aactatctgc gttttctgtt ttcccgtgtc cgaagcgggt gcgtagatac agattacctg
     3421 cgttttcaaa agcatatttg ataattggct cgtagtaagt ttcggtataa atactacgat
     3481 catttatttt accgtgatta aattcatcgt tatgcttttt ctggtagaaa aattcaatac
     3541 caaactccca tgcaccaaaa cgtttatagg gctgaaatag tatactttga ccttcttctt
     3601 cgcgtacgcc ccaggagctc ttatcatttc gactgtataa gtattcaacg tttgaataaa
     3661 aacctgcgtt ccagtcagag ttaaagtacg tcaacgtagg gcgaaaactg ttgtcaacag
     3721 ttacagtatg caagcctgta acataagggc tatctatatt tccgtcggta tacatcaggt
     3781 cgtataccag actgaagtcg aaaccgttac caatagtgaa cgttttgttt agtgatatat
     3841 agttttgcag acttgtttca ttttcgtgat agcctttttc ttcttgccgg cgatcgatag
     3901 acttaaggcc atagattgct gcaaattgcc aattaggttg gctgaaatag gcctggaaaa
     3961 tctcgtgtgt tttagtatat tcctttactg ttttacctaa atagttatca gtgcttttgg
     4021 tctcatattc aaatgtagta ccaacatgtc cattaaagcc cgcttgttct tctgaccagt
     4081 cagtgtcgga ggtaaagccg cgatcgtttg ccgcaaaggc tcctgttgag cctaaaagga
     4141 ctgttgcaat tgaagccgat aagacattct tttttattaa aaccataaat gagttccttc
     4201 caaaatatca atttaagata tgatttctgt tattccatat agtcgtacct catgtactcc
     4261 taaacactct gcggcaataa aatatttttt tcctcaacaa attactgcaa atgagatatg
     4321 catcactatt tgcagttatt catcattaaa atcttatttg acagttgatt ggccttgatt
     4381 tgtgttgata atggcgtgaa tgcttatgta ggcgtacagc atgtactact taaagatatt
     4441 attcacgagg taaatataat gagactttca aatatcgtca cagttataat tcttgcaatt
     4501 tccagtacgc taacacccca ggcgatggca gaaaaattga ttcctgaaac tgatgctgag
     4561 ttattaatct ggtctgatgc cacaagcgcg gaatatatga aatatgcagc aacggaattc
     4621 aataaagatt ttggctacaa ggtcaagttt acatttcgca atatagcgcc aatggatgca
     4681 gcatcaagaa ttatgcagga tgggggtacg actcgtgtag ctgatgtagc tgaaattgaa
     4741 catgataccc tggggcggtt agtcgttgct ggcggggtta tggaaaacat ggtctcagct
     4801 gagcggatta aaaaaacatt tattccaggc gcagtatcgg cagctacata taataacatc
     4861 agctatggtt ttcctgtaag tttcgcaacg ctggcgcttt tctataataa ggatttgtta
     4921 aacaccgcac caaaaacatt cgaagaaatc aatactttca gtgaaaagtt taataattca
     4981 tccgagcata aatatgctct gctatgggat gtacaaaatt attatgtttc acgtatgttt
     5041 attaccttgt atggtgccaa cgaattcggt aaaaccggta acgatcctaa agctctaggc
     5101 atcgcttcat ctgaagcgaa gaaagggcta gagacaatga aacgcttaaa gaaagcgaat
     5161 ccttctaatc ctcttgatat gggtaatcca caagttctaa gaggtctgtt taatgaagga
     5221 aaagttgctg ctgtaatcga cggaccttgg tccatacaag gttacattga cagcggaatc
     5281 aattttggcg tgacacgcat cccaacatta gatggtcatc agcctcgcac attttcaaca
     5341 gtacgactgg ccgttgtaag ctcatttacc gaatatcctc atgccgctga attattcgct
     5401 gactacctga caacggataa gatgttgatg aagcgttatg aaatgacgaa tctcatccca
     5461 ccaatcgatt cacttatgaa caaaattagc cagacaggta gtgaagctat aaaagcaatt
     5521 attgctcaag ctaattattc tgacgcaatg ccatcaatac cggaaatgtc ttatctatgg
     5581 tctccaatga ctaatgctat tttggctacc tgggttgaga ataaaacacc ggatgaagtt
     5641 ttaaatcatg cacaaacaat tattgaagaa caactttcgc ttcaggagta attgtgaata
     5701 cacaacaact gaaaatgaag agtgcacctg tgctacctat ttcctgtttg attatgggcg
     5761 ggactcagtt atcacgtcat tattatgtga aaggcgggat attctttgca atacaagtat
     5821 gctttttgct ttatctttcc gatattgtgc atacattaat aggtttgttc acattaggtg
     5881 atgtcgcgca aattcgaaaa gggttgactg ttattcaggg tgacaattct atatttatgc
     5941 ttgtagaggg cgtgatagcg accattattg ttggcttatt cgccactatt tacatcctta
     6001 atattaaaga tgcgcggaac tcatcttatt gccatctgac ttttaaacaa cagttatata
     6061 agctctatga ggataaattt gcatttatag tcctaacgcc cgcattttta gcgagtatag
     6121 cattcattgt tttacctatt gtaattacag ttcttgtatc gtttacaaac tatgctgcgc
     6181 caaa
//
LOCUS       DAZGJW010000218         6165 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZGJW010000218 DAZGJW010000000
VERSION     DAZGJW010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.219"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            48..824
                     /gene="exuR"
                     /locus_tag="ACHKIK_004671"
     CDS             48..824
                     /gene="exuR"
                     /locus_tag="ACHKIK_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO9085690.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1064..1147
                     /gene="yqiM"
                     /locus_tag="ACHKIK_004672"
     CDS             1064..1147
                     /gene="yqiM"
                     /locus_tag="ACHKIK_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO9085691.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1169..1831
                     /gene="yqjA"
                     /locus_tag="ACHKIK_004673"
     CDS             1169..1831
                     /gene="yqjA"
                     /locus_tag="ACHKIK_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO9085692.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1835..2218
                     /gene="mzrA"
                     /locus_tag="ACHKIK_004674"
     CDS             1835..2218
                     /gene="mzrA"
                     /locus_tag="ACHKIK_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO9085693.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2365..2733
                     /gene="yqjC"
                     /locus_tag="ACHKIK_004675"
     CDS             2365..2733
                     /gene="yqjC"
                     /locus_tag="ACHKIK_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO9085694.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2771..3076
                     /gene="yqjD"
                     /locus_tag="ACHKIK_004676"
     CDS             2771..3076
                     /gene="yqjD"
                     /locus_tag="ACHKIK_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO9085695.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3079..3483
                     /gene="yqjE"
                     /locus_tag="ACHKIK_004677"
     CDS             3079..3483
                     /gene="yqjE"
                     /locus_tag="ACHKIK_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO9085696.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3473..3772
                     /gene="yqjK"
                     /locus_tag="ACHKIK_004678"
     CDS             3473..3772
                     /gene="yqjK"
                     /locus_tag="ACHKIK_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO9085697.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3958..4350
                     /gene="yqjF"
                     /locus_tag="ACHKIK_004679"
     CDS             3958..4350
                     /gene="yqjF"
                     /locus_tag="ACHKIK_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO9085698.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4420..5406
                     /gene="yqjG"
                     /locus_tag="ACHKIK_004680"
     CDS             4420..5406
                     /gene="yqjG"
                     /locus_tag="ACHKIK_004680"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO9085699.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5699..6064
                     /gene="yhaH"
                     /locus_tag="ACHKIK_004681"
     CDS             5699..6064
                     /gene="yhaH"
                     /locus_tag="ACHKIK_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO9085700.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1543 a         1517 c         1550 g         1555 t
ORIGIN      
        1 taaagtggta tgataacttc gtccgcattc cactccggga gacgcatatg gaaatcactg
       61 aaccacgccg tttgtatcaa caacttgccg ctgacctgaa agagcgcatc gaacagggcg
      121 tatatctggt aggtgataaa ctgcctgcag aacgctttat tgccgatgaa aagaacgtca
      181 gccgtacggt agttcgtgaa gccatcatca tgctggaagt tgaaggctat gtcgaagtgc
      241 gtaaaggttc aggtattcat gtggtttcca accagccgcg ccatcagcag gcggctgaca
      301 ataatatgga attcgccaat tacggtccgt ttgagttgct tcaggctcgc cagctcatcg
      361 aaagtaatat tgccgagttc gcggcaactc aggtaacgaa acaagacatc atgaaactga
      421 tggccattca ggaacaggcg cgcggcgaac aatgcttccg tgattccgag tgggatttac
      481 agttccacat tcaggtagcc ctggcgacgc agaactccgc cctggcggcc atcgttgaaa
      541 aaatgtggac ccaacgtagc cataacccgt actggaaaaa actgcacgaa cacattgatt
      601 cccgtaccgt cgataactgg tgtgatgacc acgatcaaat cctcaaggcg ctgattcgca
      661 aagatcctca tgctgctaag ctggcaatgt ggcagcacct ggaaaacacc aagatcatgt
      721 tattcaacga aaccagcgac gacttcgagt tcaatgccga ccgctatctg ttcgccgaaa
      781 acccggtagt gcatctcgat acggccacca gcggcagtaa atgaaatacc tgtccgacag
      841 gcgctgcgac gcgcctgttt gtctcgtcgg taagcgaaaa gtataaagtg tcagcctgtg
      901 taaatcctct cgccaccctc ccctgcattc agcaaaatca gactccacgg acatgtaatt
      961 ttgataacga acaacgttga cctttgttac aattagattc aatttgaatt tatgtttttg
     1021 aatgctttct tatctcacga tttaacaggg aatagttcag gctgtgttga tgtatcaaac
     1081 ccgcagaaca taccaaaaca gcaataacat tgcggtagtg catcttttaa aaccagcgtg
     1141 gcgttaaccg attcaccagg aataatgaat ggaacttttg acccaattgc tgcaagccct
     1201 gtgggcgcag gattttgaaa ccctggccaa tccatcgatg attggcatgt tgtattttgt
     1261 cttgtttgta attttgttcc ttgaaaacgg cttgcttcca gcggcctttt taccgggcga
     1321 cagtttactg gtattggtcg gcgtgttgat tgcgaaaggc gcgatgggtt acccgcaaac
     1381 gattctgctg ctgaccgttg ccgccagcct cggctgctgg gtcagctata ttcaggggcg
     1441 atggctgggc aatacccgca ccgtacaaaa ctggctatct catttacccg cgcattatca
     1501 tcaacgcgca caccatcttt ttcataaaca cggtttatcg gcgctgttaa ttggtcgctt
     1561 tattgcgttt gtcagaacac tgctgccgac gattgccggg ttatcagggc tgaataacgc
     1621 gcgctttcag tttttcaact ggatgagcgg tctgctgtgg gtattgatcc tgacaactct
     1681 gggttacatg ctcggcaaaa cgccggtatt tttaaagtac gaggaccagc tgatgtcatg
     1741 cctgatgctg ctcccggtgg tgctgctggt gtttggcctg gcaggttctc tggtcgtgtt
     1801 atggaaaaag aaatatggaa atcgggggta agggatgcaa atacctcgca tgtcgcttcg
     1861 ccagctagcc tggtccggca ctgttttact tctggtcggc acgctgttgc tggcctggtc
     1921 cgcggttcgc cagcaagagt ctacgctggc gattcgtgcc gttcatcaag gcacaacgat
     1981 gccagacggt ttttcgatct ggcatcacct tgacgctcat ggcattcctt tcaaaagtat
     2041 cacccccaaa aacgacactc tgttaattac atttgactcc agcgaccaga gcgccgccgc
     2101 aaaagcggtc ctcgacagaa cattgcccca tggctacatc attgcgcagc aggacaataa
     2161 cagtcaggct atgcagtggc tgacccggtt acgggataac tctcatcgct tcggataact
     2221 tcctgttatc cgaaacatat cactcacttt ggtgatttca ccgtaactgt ctatgattaa
     2281 tgaggcggtg gttaacacta ccgcttcgtt gtctggacaa tcgttccttt gtaataggtc
     2341 cgaatcacaa tggaaggttc aagaatgaaa taccgcatcg ctttagctgt ttctctcttt
     2401 gctcttagtg ccggtagtta tgccactacc ctgtgtcagg aaaaggagca aaatatcctt
     2461 aaggagatca gctatgccga aaaacaccaa aaccagaatc gtattgacgg tctgaataaa
     2521 gccctgagtg aagtccgggc caactgttca gatagccagc tgcgtgccga tcatcacaag
     2581 aaaatcgcaa agcagaaaga tgaggtggcg gaacgccagc aagatttagc cgaggcgaag
     2641 caaaaaggcg atgccgataa gattgccaaa cgcgaacgga aactggcaga agcgcaggaa
     2701 gagctgaaaa agctggaagc gcgcgactac taactcacaa tagtcactac ttactcacct
     2761 ggagaaaact atgtcgaaag aacacactac ggaacatctg cgtgctgagt tgaaatccct
     2821 ttccgatacg ctggaagagg tgcttagctc atctggcgag aagtcgaaag aagagttgag
     2881 taagattcgt agcaaagcgg agcaggcact gaaacagagc cgttatcgcc tgggtgaaac
     2941 cggtgatgcc atcgccaaac aaacccgtgt cgcggcggcg cgtgctgatg agtatgtgcg
     3001 tgaaaatccg tggaccggcg tgggcattgg cgctgcaatc ggtgtagtgc tcggcgttct
     3061 gctgtcgcgt cgttaattat ggcggacact catcacgcgc aagggcccgg taaaagcgtt
     3121 ctgggcatcg ggcagcgaat tgtttccatc atggttgaaa tggtggaaac acgtctgcgg
     3181 ctggcggtgg tggagctgga agaggaaaaa gctaatctct ttcaactttt actgatgctg
     3241 ggcctgacga tgcttttcgc tgcatttggt cttatgagcc tgatggtgct aattatttgg
     3301 gcggttgatc cgcaatatcg cctgaatgcg atgattgcca ccaccgtggt gttgctgcta
     3361 ctggcactaa ttggcggtat ctggacgtta cgtaaatcgc gtaaatccac cttgctgcgc
     3421 catacacgcc atgagctggc aaacgatcgg caactgctcg aggaggagtc ccgtgagcag
     3481 taaagtcgaa cgtgaacgac gtaaggcgca actgcttagc cagatccagc aacaacggct
     3541 ggatctttcc gccagtcgtc gtgaatggct ggaggcaaca ggcgcttacg atcgtcgctg
     3601 gaatatgctg ctaagtctgc gctcctgggc gctggttggc agtagcgtga tggcgatctg
     3661 gacgattcgc catcctaata tgctggtccg ctgggccaga cgcggttttg gcgtatggag
     3721 cgcctggcgt ctggttaaaa cgaccctcaa gcagcaacag cttcgcggtt aacttccctc
     3781 tggccggagc aattccggcc ttatccctca aatttcttga agatttttga cagttttcct
     3841 tgctaacaat catcattcac cacgtttatg attctctcca tcgacagcaa cgacgctaat
     3901 accgcgccct tgcacaaaaa aacaatcagc agcctgagtg gcgcagtgga gagtatgatg
     3961 aaaaaattag aagatgttgg tgtactggta gcgcgcattt taatgccgat tctgtttatt
     4021 accgcaggct ggggaaaaat caccgcttac tctggtaccc aacaatatat ggaagcaatg
     4081 ggtgtcccgg gttttatgct gccgctggtg attctgcttg agttcggtgg tggtctggca
     4141 atcctgttcg gtttcctgac tcgcaccaca gccctgttta ctgcgggctt tacgctgctg
     4201 acggcatttt tatttcacag caactttgct gaaggcgtca actcgctgat gttcatgaaa
     4261 aacctgacaa tttctggcgg attcctgctg ctggcaatta ccggtccggg cgcgtatagc
     4321 atcgaccgcc tgctgaataa aaagtggtaa gcacgcgcta tacttaacga taaaaaacga
     4381 ggaggaagct cctcgttttt gctattggag gagagaaaaa tggggcaact gattgacggc
     4441 gtctggcatg acacctggta cgataccaaa tctaccggcg gtaaatttca acgttcagct
     4501 tccgcatttc gtaactggct cactgccgat ggcgcacctg gccccactgg cacaggcggt
     4561 tttatcgcag agaaagatcg ttatcatctc tatgtttcac tcgcctgccc gtgggcgcac
     4621 cgcacgctga tcatgcgcaa actcaaagga ctggaaccgt ttatttccgt ttccgtagtg
     4681 aacccgctga tgctggaaaa cggctggacc tttgatgaca gttttccggg agcaaccgga
     4741 gatacgctct atcaacatga atttctgtat cagctttatc tccacgccga tccacactac
     4801 agcggacgag ttactgttcc cgtgctgtgg gacaaaaaga accacaccat cgtcagcaac
     4861 gaatcagcag aaatcatccg catgttcaat accgcgtttg atgcgctggg cgcaaaagcg
     4921 ggtgattact acccaccagc cctgcaaacg aaaattgacg aacttaacgg ctggatttat
     4981 gacaccgtta acaacggcgt gtataaagct ggttttgcca ccagccagca agcctacgac
     5041 gaggcggtgg cgaaagtgtt tgaatcgctg gcgcgactgg agcagatttt aggccagcat
     5101 cgttacctga ccggcaacca gctaaccgaa gccgatattc gcctgtggac cacgctggtc
     5161 cgttttgatc cagtgtatgt gacccacttc aagtgtgata agcaccgcat cagcgattac
     5221 cttaatctgt atggcttcct gcgtgatatc taccagatgc cgggaattgc cgaaacagtc
     5281 aatttcgatc acatccgtaa tcattacttc cgcagccata agaccatcaa ccctacgggg
     5341 attatttcaa ttggtccatg gcaggatctc gatgaaccgc atggacgaga tgttcgcttc
     5401 ggttaaaaaa aggcacctta cgaggtgcct ctgataatta gctaagtgca tgttgttcat
     5461 gtcggatgcg gcgtgaacgc cttatcaaac ctacaaaaca tttatattca atatgttgca
     5521 atgaccgcgc aggccagata agcgtagcgc atctggcgat tttgcgcttg tcatcagtct
     5581 tagcgcgcct tcacagcact ttattaattc cttgaacgaa tatttactgc catttcattc
     5641 acgtctattc ttaatttgct gctttaaaaa caagtgattg agcaaaattg aggcaaaaat
     5701 ggactggtat ctgaaagtac taaaaaatta tgtcggtttc cggggtcgtg cgcggcgcaa
     5761 agagtactgg atgtttattc tggtcaacat catctttacg ttcgtgctgg ggctgctgga
     5821 taaaatgtta ggctggcaac gcgctggtgg cgaaggcatc ctgacgacaa tctacggtat
     5881 tctggtgttt ttaccgtggt gggcagttca gttccgccgc ctgcacgaca ccgaccgctc
     5941 ggcgtggtgg gcactgttgt tcttaatccc gtttatcggc tggcttatta tcatcgtctt
     6001 taactgccag gcgggtacgc caggcgaaaa ccgctttgga ccagatccga aactggaacc
     6061 ttaaaacaaa tctgctcgcc ggatgtctga ctcatccggc ttttatcatc atgttgatga
     6121 atgaataatt tactaatgtt tatttaaaat atttcaatgt cgtta
//
LOCUS       DAZGJW010000219         6108 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZGJW010000219 DAZGJW010000000
VERSION     DAZGJW010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.221"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            418..1434
                     /locus_tag="ACHKIK_004682"
     CDS             418..1434
                     /locus_tag="ACHKIK_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085701.1"
                     /translation="MSSVRTDKFIRYGFAVVAIVAILITTITTPNPTPYQYIVYRIVL
                     VLAAGCIGAVLPGFLEVKFNGWLRASGAVAIFAIVFFISPDLLQQNPESASKPVPPTV
                     IDAYAVNSSNRDRYLELFRTGQFGQRDKLRIGCVEWSESSCVAAGNFLRLLSEAGWEI
                     DSDAVFRMQPSVPVEGVSIVSRGDDLVGLPKVPPHMGRWSRSDISYEILTAAFKLMGV
                     PVYFSSDPTLQPGTTGVYFGPKPKLDAPVMQKDENVRRELLRYVGLGMTIQRACSQQP
                     VELCGGEIFSWETSVSSFLRQQRFNSDAVNRWLALPQVNAASATADMKIKLDLLMSFV
                     LGIE"
     gene            2078..4438
                     /locus_tag="ACHKIK_004683"
     CDS             2078..4438
                     /locus_tag="ACHKIK_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HFO9085702.1"
                     /translation="MKSMQSKTGSPLVRTEAELESRLTSALNIAFPNIPREDLIEQRH
                     FTVRLGHGTYKIDSAAHWKKYGRADVLIFHRERPLAVIELKREDLTLTHDDYEQAQSY
                     ANQLTPRPPLVVVTNGKDTRVYDSSNGQQWSGGQDASAAVNKMLANSAKLAAADMRWA
                     IEALMGRETNAWVPAVREETARLLIDITDQPGHSEHPFANNLLFPRKITSLVIESAVM
                     GTAFTIIEGDAQSGKSSCLREISLKTESSDLLAVLMLRGSGPGLFQALANLFAAEFEW
                     NLTSNDARNWLRRMSNCTEGPSLMLAIDDVEPGSQMATDLEELAGIRFGNRLVVVLTT
                     YHANALLKNPNGRTPSAIGSRSKVFKTSPMSLDEFKLAQQILSDQRIVFQQGAEYADD
                     YRSPWVLRTIYDDIVRNHQYQKTDLIAYLPPSPGMELIDAAQKSYESQYDLLRYYRVL
                     ARCALADTNSHSVELMFAKANGFVVRYDALSDEARGVVNELKHMGAVRIFRLSGWEDV
                     VVPTVPAAYLLELSDAVCDELVLRAEQDPQDAGAWLGERLDATYLGDMIGAQAIRRMA
                     AKERYFSFGIIQGLLSIEPYKEPIKNGLFTLAMPDNQQVNLKIEDGLAWISKHGDDAK
                     SVLVNLEDQIPKVISKSTSWMILGQLAKLPSAEVGDDDQRIDAYILLSIGRCPFPLIR
                     TNIEGLPYFEHNFGDQGDVLCLEKASIEVATQAMADLFSAPWLYADQWVDTAIATGSI
                     HLLHRLFAALNTVILRRIPVQSDWANEALNQRVSPALKEAIRSLSS"
     gene            4615..5754
                     /locus_tag="ACHKIK_004684"
                     /pseudo
     CDS             4615..5754
                     /locus_tag="ACHKIK_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5751..5906)
                     /locus_tag="ACHKIK_004685"
     CDS             complement(5751..5906)
                     /locus_tag="ACHKIK_004685"
                     /inference="COORDINATES: protein motif:HMM:NF015301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PapB/FocB family fimbrial expression
                     transcriptional regulator"
                     /protein_id="HFO9085703.1"
                     /translation="MTKGNISERDFDILIKLSGIHSEKAILALKKYYVDGVIRKIDPT
                     HVMWTRP"
BASE COUNT         1598 a         1352 c         1494 g         1664 t
ORIGIN      
        1 cttacttggc tttagtcccg gagtgttgcc aggagtatct ttattatctc gtcggcgttg
       61 atctggtttt cctgtcgatt tagcaattgg ttttggacca ggtttaagac ccatgatagt
      121 tatccttttg agatgtaaga gggaagccct caacattggt atattggata ctgagaaatt
      181 tttcaatggg agtgataaac ggccgaatga ttaactcgat attgtgatca acgtctgttt
      241 tttgcacact gtagactgac tcttggagca gaaggttgct ctgagcgagg aatggacagt
      301 tacactcatc accaacgcca atagcctcat tacgcgtata aagctgtatt aatcattggg
      361 gagcagttta atgcgggatt ttcgacttat ggacttgatt caggtgtcaa aattaaaatg
      421 agcagtgtaa ggacagacaa gttcattagg tatggttttg cggttgtggc gatagttgcc
      481 atactcatta ctactatcac aacccccaat cccacacctt atcaatatat agtttaccga
      541 atagttctgg ttctggctgc cggatgtatt ggtgcagttc tgcctggttt ccttgaagta
      601 aaatttaatg ggtggttaag agcatctggc gcagtggcga tcttcgctat agtgtttttc
      661 ataagcccag atttgcttca acaaaatcct gaatctgcaa gtaaacctgt accgcctact
      721 gtgattgatg catatgccgt taatagttcc aatcgcgaca gatacctcga attatttcga
      781 acaggacagt tcggtcagcg ggacaagctg cgcatcggtt gtgttgagtg gagtgaatca
      841 tcgtgcgtgg ctgctggtaa ttttttgcga ttgttaagcg aagctggctg ggaaatcgat
      901 tctgacgctg tattcagaat gcaaccatcc gtgccagttg agggggtatc gattgtaagc
      961 cgtggcgatg atctggtcgg tttgccaaaa gtaccacccc acatgggccg ttggagtcga
     1021 tcagacatta gttatgaaat tttgacagcg gcatttaaat tgatgggagt tccggtttat
     1081 ttttccagtg acccgacatt acaaccaggt actaccggag tgtactttgg accgaaaccg
     1141 aaactggatg caccagtcat gcaaaaagat gagaatgtgc gacgtgagct tctccgctac
     1201 gtcggtttgg gaatgactat tcagcgagcc tgctctcagc agcccgtcga actttgtggg
     1261 ggtgaaatct tttcctggga aacgtcggta tcttcgttcc taaggcagca gagattcaac
     1321 tccgatgccg taaacagatg gcttgccttg ccgcaggtta atgcggcttc ggcaactgca
     1381 gacatgaaaa taaagctaga tttgttaatg tcatttgttc tgggcatcga gtaactatac
     1441 ctcttgcgag gatgattgag cgtctaatgg accagcttag ccgttagcgc tatttgttta
     1501 cgtaagcctg tggaatccaa ctagtgcttc tacgatgcgt tgtttggtga aaaaaaagtg
     1561 ttgaagcact ggttttagac aactaaagcc acgatttgct catgcgtcga aaatgtagtc
     1621 gtctactgtg tagcacggag gcgatattcg tacgcttaca cttgccgata ggccactggg
     1681 gagtgttctt ggcactaagc tgccaggccc gaaggtctgc tatgagcgag gggctgacat
     1741 tcctattggc attcggtgtg agttatcggg ggcggtcatg cggcatactt ccgatacagg
     1801 cgtttccagc tctgcctgtt tcagggttat attaatctgc tttagaatta atcgtggctt
     1861 tagcatctgc acatcgtgct ggagaaacct gagtgaacta aatcatattc cctttcttca
     1921 gctaatcaat tgatagttat gcggaaaaag ttactttgct tgtcaataat ccatttcaca
     1981 tccgttaatc caatgctatg cgtcttcgca ttaatctgga aaggtagtaa gtgaaataaa
     2041 aacttagcca atgactaata tctgaggata ctttgtcatg aaaagtatgc aatcaaaaac
     2101 gggctctcca ctcgttcgaa ctgaagccga gctagaaagc cggcttactt cagcactaaa
     2161 catagcattt cctaacattc ctcgcgaaga tcttatcgaa cagcgccatt ttaccgttcg
     2221 gctaggccat ggaacttaca aaatagatag cgccgcacac tggaaaaaat acgggcgagc
     2281 ggatgttttg atctttcatc gggagagacc acttgcggtt attgaactga aacgtgaaga
     2341 cctgacactg acccatgatg attatgaaca ggctcaaagc tacgctaacc agttaacacc
     2401 acgtccgcct ctcgttgtag ttacgaacgg taaagatacg cgcgtttatg actcaagtaa
     2461 tggacaacag tggtcgggtg gacaggatgc ctcggcagcg gtaaacaaga tgctcgccaa
     2521 ttctgcaaag ttggcggcgg ctgacatgcg ctgggctatt gaagccttga tggggcgaga
     2581 aactaatgct tgggtgccag ctgtgcgaga agaaacagcc cggctgctga ttgatataac
     2641 tgatcagcct gggcattcag aacatccatt tgccaacaac ctactttttc ctcgcaaaat
     2701 cacgtctctt gtaattgaat ctgctgtgat gggtacagct ttcactatca ttgagggtga
     2761 tgcacaaagc ggaaaaagca gttgcctccg tgaaatttcg ctaaaaacgg aaagtagcga
     2821 tcttttggct gtcctgatgt tacgaggttc cggaccagga ctatttcagg ctctggccaa
     2881 tctttttgca gctgaatttg aatggaattt aacctcaaat gatgcacgta actggttaag
     2941 acgaatgtct aactgcactg aaggtccatc gcttatgctt gctatcgacg acgttgaacc
     3001 aggctcccaa atggctactg atcttgagga acttgcgggt atacgttttg gcaacaggct
     3061 ggtggttgtt ctgacgacct atcatgccaa cgcgttactg aaaaatccaa acggacgaac
     3121 tccgtctgca attggtagcc ggtcgaaggt atttaaaaca tcgcctatgt ccctagatga
     3181 atttaagctg gcgcaacaga ttctctcgga ccagcggata gtttttcaac agggagcaga
     3241 atacgccgat gactaccgct cgccttgggt tcttcgcact atttatgatg acattgtgcg
     3301 caatcatcaa taccagaaaa cggatcttat cgcctatctt cctccatcac ccggtatgga
     3361 acttatagat gctgcgcaaa agagctatga gagtcagtat gatcttcttc gttattatcg
     3421 ggttcttgca cgctgcgcac tagctgacac aaactcccac tcagtagaat taatgtttgc
     3481 taaagcgaat ggatttgtcg tgcggtatga cgcactttct gacgaagctc gtggtgtagt
     3541 gaatgagtta aaacatatgg gggcagttcg tatttttcgc ctttcaggat gggaagatgt
     3601 tgtggtgccg acagttcccg cagcttacct tctggaactg tcggatgctg tttgtgatga
     3661 actggtttta cgggcagaac aagaccctca agacgccggg gcttggcttg gcgaacggct
     3721 agacgccact tatcttggag atatgatcgg tgctcaagcc atacgccgta tggcagctaa
     3781 agagagatat ttcagctttg gaattattca gggccttctc agcattgagc catataaaga
     3841 acctataaaa aacggattgt ttactttagc catgccagac aaccaacaag tcaatctaaa
     3901 gattgaggat ggattagcgt ggatttctaa acacggggat gatgcaaaga gtgttctggt
     3961 gaatttagag gatcaaatcc caaaggtcat ttctaaatca accagttgga tgatactcgg
     4021 gcaactagcg aaactgccgt cagcagaggt gggtgacgat gatcagcgta tagacgccta
     4081 tattttactg agcattgggc gctgcccatt tcctctaatt cgcacgaata tagaaggatt
     4141 gccttacttt gagcataatt ttggcgatca gggtgatgtt ctttgcctag aaaaagcgtc
     4201 tattgaagtt gctactcagg cgatggctga tttattttca gccccatggc tctatgcgga
     4261 ccagtgggtg gatacagcta tcgcgaccgg ttctatacat ttactgcatc gtttgtttgc
     4321 tgctttaaac acagtgatat tgcgtagaat acccgtgcaa tccgactggg ctaatgaagc
     4381 acttaatcaa cgtgtctcac cagcccttaa ggaagccata cgatcattat cttcgtgact
     4441 aagcaatggg attttaatac ataacgagca tctgtccacg caagttaaca attgccttgg
     4501 ctcgatattt tggtttctag tgcatatatg ctgatcttct cttcgttgat ctgatcttac
     4561 ccaccaatag tggacacagg actaagtgag taaactctca accagaggtg actcatgaca
     4621 aaaccagtat caatcagcaa gaagccccgt gaacaacatt cgcctgaatt tcgtaacgaa
     4681 gccctgaaac tcgctgaacg catcggtgtg gccgccgcag cccgtgaact cagcctttat
     4741 gaatctcagc tttatgcctg gcgcagtaaa cagcagcaac aaatgagttc gtcagagcgc
     4801 gaaagcgaac tggccgctga aaatgtccgc cttaaacgac aactggcgga gcaggctgag
     4861 gaactggcca tcctccaaaa ggccgcgaca tacttcgcga atcgcctgaa atgaagtatg
     4921 tcttcatcga aaatcatcgg gcagagttct gcatcaaagc gatgtgtcgt gtacttcggg
     4981 ttgcccgcag cggctggtat gtctggctca ggcgtcgtca ccagatgagc cttcgccaac
     5041 agtttcggct cacctgcgat gccgctgtcc ataaggcatt ctttgaggca aagcagcgat
     5101 acggtgctcc ccgccttgct gacgaactgc cggagttcaa tattaaaacc attgccgcca
     5161 gcctgcgtcg tcaggggctg cgggcgaaag ttcagcccgg tcagctaccg tgcacatggc
     5221 ctgcccgtat tggagaatct gctggagcag gacttcagcg ccagaggccc gaaccagaag
     5281 tgggcgggtg acatcacgta cttgcgtacc gatgagggct ggttgtatct cgcagtagtc
     5341 atcgacctgt ggtcacgcgc cgtcattggc tggtcgatgt caccgcgaat gacagcacaa
     5401 ctggcctgtg atgcactaca aatggcgttg tggcggagaa gacgcccgga aagcgtcatt
     5461 gttcatacgg accgtggtgg tcaatactgt tcaggggatt atcaggcgct gctgaagcga
     5521 cacaacctgc gtggcagtat gagtgcgaaa ggctactgtt atgacaatgc ctgtgtggaa
     5581 agcttctttc attcgctgaa ggtggaatgt atccacgggg aacgctttag cagccgggaa
     5641 ataatgcggg caacggtgtt taattatatc gagtgtgatt acaatcgctg gcgtcgtcac
     5701 agtgcctgtg gcggtctcag cccggaacaa tttgaaaacc ataatctcgc ttagggccgt
     5761 gtccacatta cgtgggtagg atcaattttc ctgatgaccc catcgacata gtattttttc
     5821 aaagcaagaa ttgccttctc gctatgtatt cctgatagct tgatcaggat atcaaagtct
     5881 ctttcagata tattcccctt tgtcatgtgt cactccatga atcaaatttc atagtaatca
     5941 atctttcttt gatcgactta tagataataa gatcagaaat gataaaaaag taacgcatat
     6001 ttcttctcgt catgtctact tataaaattc aaatgaacta acctgaacta cttacactgg
     6061 aatatcatta tattggaata attataaaac tgaacaaaat ggtcaatg
//
LOCUS       DAZGJW010000220         6097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZGJW010000220 DAZGJW010000000
VERSION     DAZGJW010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.222"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..1222)
                     /locus_tag="ACHKIK_004686"
                     /pseudo
     CDS             complement(1..1222)
                     /locus_tag="ACHKIK_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
     gene            1205..1903
                     /gene="tagH"
                     /locus_tag="ACHKIK_004687"
     CDS             1205..1903
                     /gene="tagH"
                     /locus_tag="ACHKIK_004687"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO9085704.1"
                     /translation="MSDPASNGGINTPFMDLPPIYASPGDRNDDVSAAEMAQRHLAVT
                     PLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLDLQQQRNSLSDKHLRPLEDNP
                     LRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVRHHEEANRAAIVESLRVLLDA
                     FSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEELASDRQQGFEMLFNEVYAQV
                     YDRVLREKQREPEA"
     gene            1900..2424
                     /gene="tssJ"
                     /locus_tag="ACHKIK_004688"
     CDS             1900..2424
                     /gene="tssJ"
                     /locus_tag="ACHKIK_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO9085705.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKKYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            2427..3757
                     /gene="tssK"
                     /locus_tag="ACHKIK_004689"
                     /pseudo
     CDS             2427..3757
                     /gene="tssK"
                     /locus_tag="ACHKIK_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            3762..4523
                     /gene="icmH"
                     /locus_tag="ACHKIK_004690"
     CDS             3762..4523
                     /gene="icmH"
                     /locus_tag="ACHKIK_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO9085706.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            4532..>6097
                     /locus_tag="ACHKIK_004691"
     CDS             4532..>6097
                     /locus_tag="ACHKIK_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9085707.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGHVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQ"
BASE COUNT         1288 a         1778 c         1736 g         1295 t
ORIGIN      
        1 ccggtgcgag ggtgcgcacg ctctccagcc tgcccgtggc actgtagccg tattcccgcg
       61 tctgtcgcgg gccgctgatg cgcaccaggt cgccgttgtc attccacccg tagtcacggt
      121 catacaccag gctgttcagg tgctggctct gtaactggcc tgcgggggtg tatgtgctgg
      181 tcagttcata tgcggcatta ctgcctgccc tgctgccgaa gctgcgcacc gtctcacggt
      241 gcagcctgtc ccgcgtatac tcgaccagcg gcgtcccgcc cagcttcatg cccgccaggt
      301 aaccgctgcc gtaggtcagc cactccaccg gcggcaggct gtccggcgtg acgcggtttg
      361 ccagcccctg ttcgttgtag gcgtgtttcg tctcatgctg ccacagcagt tcccccgtct
      421 ccgggttctc caccgtctgc cgttcgcccg tcaggcggcc cttatcgtcg tacccgtagt
      481 ggaccgacac gcggtggcct tcactggtat ggctgagggt ggttaaccac ccgtgctcat
      541 catactgcca ctgctctgcc gggtcgccgt tcaccgtccg gtgcgtgatg cggtccgacg
      601 catcgtagtg ccagagggtg ataagcccct cgtcttcact ctgtgtgagt tttctggtca
      661 ggtcatagtg gtaacgttgc gtccggccgt caaaaccgcg ctgttcagtc agcctgtcaa
      721 ccgggtcata ccggaacgtg gactggctgc cgttctcgtt ggtcagcacg gtgatgcgcc
      781 cggcagcgtc ataccccatg ctgcgcgtca gaccgccttc cgtcacactc accggacgcc
      841 cgcgtttatc atatgcgatg gtgctgcgtt taccgtccgg tgataccgtt gctgtcaggt
      901 ctcctgcggc gctgtactca taacgggttt cacgcccctg cgcatccttc tgactgacca
      961 gttgcccacg cgggttataa ctgctgtaag tgctgatgcc ttcctcccgg tgaacggcga
     1021 tttgttgacc gtaccggtcg tactcatacc gcgtcgtgta ccccgagcag tccgtaaagg
     1081 ccagcagttg accgtaacgg ctccatgcca tctgttttgt actgcccgtc gcgtcctgta
     1141 tccctgtcgg cagctcactc gccggccgct ttcaggcatt gcggctgtcc ggccatcggc
     1201 accgctgtcc gatccggcaa gtaatggggg gatcaatact ccgtttatgg atctgccgcc
     1261 catttatgcc agccccggcg atcgtaatga cgacgtctct gcggcagaaa tggcgcaacg
     1321 ccaccttgcg gtcaccccct tactgcgcgg tcttggcggc tcgcttacca tgagcaattc
     1381 cgacgatgcg gatgattttc tggaggaggc cggacgaacg ttacaggccg caataaaagg
     1441 tctgctcgat ttgcagcagc agcgtaacag cctctcagac aaacatttgc gcccgctgga
     1501 agataacccg ctgcgcctga acatggatta cgccaccgcg ctcgacgtaa tgtttgccga
     1561 aggtaaaagc ccggtacatc tggcggctcc cgccgccgtc agtgaaagcc tgcgcaatgt
     1621 ccgccaccac gaagaagcta accgggcagc gattgtggag tcgcttcgtg tcctgctgga
     1681 tgctttctca ccacaaaatc tgctgcgccg ctttgtgcag taccgccgca gccatgaact
     1741 gcgccagccg ctggatgatg ccggagcatg gcaaatgtac agccattatt acgaagaact
     1801 ggcctccgat cgccagcagg ggtttgagat gctgtttaac gaggtctacg cccaagtcta
     1861 tgaccgggtg cttcgtgaaa aacagcggga gccggaagca tgaacaggaa ggcgtttctg
     1921 gcctgcgtgc tgatgtgcgt catattaacc ggctgcgaaa cggcgaaaaa aatcagtgag
     1981 gtgatcaaaa acccggatat ccaggtcggg agcctgaagg agcaaccttc ggaaataacc
     2041 gtcaccctgc tgaccgaacc ggacaccaat accaatgcgg aaggtgaatc agccgccgtg
     2101 gatgtgcaac tggtgtacct gactgacgac tcaaaattgc tggcagccga ctatgaccag
     2161 atagccagta cgccgctacc ggatgtgctg ggtaaaaaat atatcgacca ccaggatttc
     2221 aacctgttgc cggacaccat caaaaccctg ccgccggtaa agctggatga gaaaacgcag
     2281 tttattggtg tcgtggccta tttctccgat gaccaggcca ctgaatggaa acaaatcgag
     2341 acggtggaag gaaccggcca ccattaccgc ctgctggtgc atgtacgtca aagcagcatc
     2401 gaaatgaaaa aagaggatga ataattatgg caaccacccg caacaaggtg atgtggcagg
     2461 aagggatgct gatgcgtcca caccatttcc agcagcagca gcgttacaac gactacctgg
     2521 ataaccagcg tttccgggcc atgaatgatt tatcctgggg atttaccgaa ctcactctca
     2581 acaatgaact gctggcacag ggtaagatca tgattgacag cgcgtcaggc acactgcccg
     2641 acggcaccgt cttttctatc cccgaccagg acgcactgcc cgatccgctg cacccgcaac
     2701 attttccgga cgagagaagc cgcaatatct acctcgctct gccggtcgcc agtgatgtga
     2761 gaaatgaaat cagcgacggg cggcgaatcg ggcgttaccg gctgaattat gccgatgtcc
     2821 gggatttgca ttcagaagaa ggtgacacgc gaacactgac gctgggacaa ctgacgccgc
     2881 gcatatgagc ggtgcagaag atatgagcgc ctatattacg ctcccgcttt gccgtatcag
     2941 cgatcgccat gccgatggct ccctgacgtt ggatgacgat tttatcccct cctgccagaa
     3001 tattcaggtc agtaagaaac tgcgtgttta tctcaaagag gtacaggggg ccattggcgg
     3061 acgggcaagc gatctggcaa accgtattgg ctctccggcg cagagcggca tcgcggatgt
     3121 ggcggaattt atgatgttgc agttacttaa ccgtaaccag acccggttta cccatcgcgc
     3181 ccgccgatcc cagctccacc cggaagattt ctaccttgat cttgctgggt tgctgggtga
     3241 actgatgacc tttaccgagc cgtcgcgcct gccctgcccg cttgatgtgt atgatcatca
     3301 tgatctgacc aaaacattta aaacactgtt accggaagtc aaacgggcgc tgcataccgt
     3361 actgtcgcca agagcggtca atctgccgct gcatctgcgt gacggtatct ggcaggccga
     3421 tatccatgac acagaactgc tgcaatctgc cacctttgtg ctggccgtgg cggcaaacgt
     3481 gccggtcgat cagatccagc gtcagtttat ccagcagtcg aaaatttcct cgccggaaaa
     3541 aatccgcaat atggtcagtg tgcagatccc cggcattcca ttgcgcgcac tgatggtggc
     3601 tccccgccag cttccttacc attccgggtt cagctatttc gaactcgaca agagcggaca
     3661 ggcctggaca gaaatggctg ccgccggggc cgttgcactg catgtatccg gcagtttccc
     3721 ggatctgaac atgcaactgt gggcgataag agggtaagac gatggatgag gggtcactgt
     3781 ctctgccacc atttaccgga tacgacgaaa aaagccagcg cgactatcat ctcgcgcttc
     3841 gtggcaacag cctgaatccg atgatcgacg cagccacgcc gctcctcggt atggtgatgc
     3901 gcctttcaac catgaacagc cagacgatgc cggagcatct ttttgctcag gtggtgacgg
     3961 atgttcaggc cgtggagcaa ctgcttcagg agcagggcta cgaaccgggc gtgattatct
     4021 cgtttcgcta catcctctgc acgtttattg atgaagcggc gctcggtaac ggctggtcga
     4081 acaagaacga gtggatcaag cagtcattgc tggtccattt ccacaacgaa gcctggggtg
     4141 gagagaaagt tttcatcctg ctggagcgcc tgatccgtga acccaaacgt taccaggatc
     4201 tgctggaatt tctctggctc tgtttttctc tgggatttcg cgggcgctac aaagtggcag
     4261 cacaggatca gggggaattt gagcagatct accgccgcct gtaccacgtc ctgcataaac
     4321 tacgcggcga tgcccctttc ccgctgttac accaggacaa aaaaacccag ggcgggcgtt
     4381 atcagctcat cagtcgcctg accattaaac atattttttg tggtggcgtt gtcgtgctgg
     4441 cgttgtttta cctgttctat ctgttgcggc tggacagcca gacgcaggac atcctgcacc
     4501 agctaaacaa attacttcga taaggacgtt tatgatccag attgatcttc ccacgctggt
     4561 aaaacggctt aacctgttct cccgccaggc gctggagatg gccgcctctg aatgtatgag
     4621 ccagcaggca gcggaaatta ccgtcagcca tgtgcttatt cagatgctcg ccatgccacg
     4681 cagtgacctg cgggttatta cccgccaggg cgatattggc atggaagagt tgcgccaggc
     4741 gctgacggtg gagaactaca caactgcccg ttctgcggac agctacccgg cattttcccc
     4801 gatgctggtt gagtggctga aagagggctg gctgctggcg tcggctgaga tgcagcacag
     4861 cgaattgcgt ggcggcgtgt tgctgctggc cctgctgcat tcgccgctgc gttatatacc
     4921 gcctgctgcc gcccggctgt tgaccggcat taaccgtgac cgtctgcaac aggactttgt
     4981 gcaatggaca caggagtcgg cggaatcagt cgtgccggat gcagacggta aaggcgcagg
     5041 cacactgacg gacgctgctg acaccctgct tgcccgctac gccaaaaaca tgaccgcaga
     5101 cgcccgtaac ggcaggcttg acccggtact gtgccgcgac cacgaaatcg acctgatgat
     5161 cgacattctc tgccgccgcc gcaagaacaa cccggtggtg gtgggcgaag caggcgtggg
     5221 taaaagcgcc ttaatcgaag ggctggcgct gcgcatcgtg gcaggccatg tgccggacaa
     5281 gctgaaaaac accgatatca tgacccttga cttgggcgca ttgcaggccg gagcgtcggt
     5341 gaagggtgaa ttcgaaaaac gtttcaaagg gctgatggca gaggtcattt cctccccggt
     5401 gccggtcatt ctgtttatcg acgaagcaca taccctgatt ggcgcgggca accagcaggg
     5461 cgggctggat atctccaacc tgctcaaacc ggcgctggcg cgcggcgagc tgaaaaccat
     5521 cgccgccacc acctggagcg agtacaaaaa atacttcgaa aaagatgccg ccctgtcgcg
     5581 ccgcttccag ttggtgaagg tcagcgaacc caacgctgcc gaagccacca ttattctgcg
     5641 cggtctgtcg gcggtctatg aacagtctca cggcgtgctg attgatgatg acgccttgca
     5701 ggccgctgcg acattaagcg agcgttatct ctccgggcgt cagttaccgg acaaagcgat
     5761 tgatgtgctg gataccgcct gcgcccgtgt ggccatcaac ctgtcgtcgc cgccgaagca
     5821 aatctcggcg ctgaccactc tgagccacca gcaggaggcg gaaattcgcc agcttgagcg
     5881 cgagcttcgc atcggactgc gtaccgacac atcacggatg accgaggtgc tggtgcagta
     5941 tgatgaaacg ctgacggcgc tggatgaact ggaagcggcc tggcaccagc agcagacgct
     6001 ggtccgggag attattgcgc tgcgccagca gttactgggc gtggcagagg acgatgcggc
     6061 gccgttgccg gacgcagata ccgtggagga tacgcag
//
LOCUS       DAZGJW010000221         6091 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZGJW010000221 DAZGJW010000000
VERSION     DAZGJW010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.223"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..626
                     /locus_tag="ACHKIK_004692"
     CDS             <1..626
                     /locus_tag="ACHKIK_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO9085708.1"
                     /translation="CNGNYMQNFTRMKPLDEDTYAGIKDYPVCFYGPKYKIMANHHGI
                     TFDDQRKRYIIGPITVSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSF
                     NRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHA
                     GRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            816..929
                     /gene="yhiY"
                     /locus_tag="ACHKIK_004693"
     CDS             816..929
                     /gene="yhiY"
                     /locus_tag="ACHKIK_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO9085709.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            992..2059
                     /gene="yhiI"
                     /locus_tag="ACHKIK_004694"
     CDS             992..2059
                     /gene="yhiI"
                     /locus_tag="ACHKIK_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO9085710.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSESRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2056..4791
                     /gene="rbbA"
                     /locus_tag="ACHKIK_004695"
     CDS             2056..4791
                     /gene="rbbA"
                     /locus_tag="ACHKIK_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO9085711.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDSLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDITHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4791..5915
                     /gene="yhhJ"
                     /locus_tag="ACHKIK_004696"
     CDS             4791..5915
                     /gene="yhhJ"
                     /locus_tag="ACHKIK_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9085712.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
BASE COUNT         1382 a         1547 c         1738 g         1424 t
ORIGIN      
        1 attgcaatgg taactatatg cagaacttca cgcgcatgaa accacttgat gaggatacct
       61 acgctgggat aaaagattat cctgtatgtt tctatggccc caaatacaaa ataatggcaa
      121 atcatcatgg gataactttt gatgatcagc gaaaacgtta cattatagga ccgataacag
      181 tctctagtga tgaaatgacc aatgctctcg atccggtcga attagcggca attatcaata
      241 aaaagcacca tgctgtaatt aatggcaaaa agtttaaaaa tagccgggct atctcttgtc
      301 gatcctttaa tcgttatttt tctgtttcga cagattacag acctaaactt gaagcactat
      361 tagcatgctc acaaatgtta ggtataaatc aagttgttgc acataacggt aatggtggaa
      421 gagaatgtat aggtgaaacg gggacagttt taggtcttaa cgcccgtgac agtaaacatg
      481 ctggaagaat gttcagcatg cataattgcc aaattaaccc tggtgcaggg cctgagataa
      541 ccactccctg gaagtcttac cagcatgaaa aaaacagaaa tggacttatg cctttgatta
      601 gaaggcgcac aatgttacag ctctgaacca tagacaacat gaatattaat gttactatct
      661 ggttgagact gttgataaaa cgtaaaaagg aatgctctgt tcctgcaact gaacaggcgt
      721 gaaacgcgat ccaacaggat ggataccgtt ttgtcagcca gttttataga tcacacttat
      781 gagtatgcgt gtttcattta cggccaggaa tgctgatgat gacaacatta ctacccgttt
      841 tcactaagcc ttccccgttg gcgctcaatg ctctgcgcgc tggtcgtatt tgtcgtttcc
      901 ttcttatccc ggatggaaga atccgctgaa cgtcgttcgg cggcagttgt aacacgcgtc
      961 gtttgcttac agggattatg gcgggccgat tatggataag agtaagcgcc atctggcgtg
     1021 gtgggttgtc ggggcactgg cggtggctgc cgtcgtggcg tggtggctgt tgcgcccggc
     1081 gggtgtgccg gaaggctttg ctgtcagcaa tgggcgcatt gaagcgacgg aagtggatat
     1141 cgccagcaaa attgccgggc gtatcgacac cattctggtg aaagaaggcc agtttgttcg
     1201 cgaaggtgaa gtgctggcga agatggatac tcgcgtgttg caggaacagc gactggaagc
     1261 catcgcgcaa atcaaagagg cacaaagcgc cgttgccgcc gcgcaggctt tgctggagca
     1321 acgacaaagc gaaagtcgcg ccgcgcagtc gctggttaat caacgccagg cagaactgga
     1381 ctccgtagca aaacgtcata tgcgttcccg ttcactggcc caacgagggg ctatttctgc
     1441 gcaacagctg gatgacgacc gcgccgccgc tgagagcgcc cgagctgcgc tggaatcggc
     1501 aaaagctcag gtttctgctt ctaaagccgc tatagaggcg gcacgtacca atatcattca
     1561 ggcgcaaacc cgcgtcgaag cggcacaagc cactgaacgg cgcattgccg cagatatcga
     1621 tgacagcgaa ctgaaagccc cgcgtgacgg acgcgtgcag tatcgggttg ccgagccagg
     1681 ggaagtgctg gcggcaggcg gtcgggtgct gaatatggtc gatctcagcg acgtctatat
     1741 gaccttcttc ctgccaaccg aacaggcggg cacgctgaaa ctgggtggtg aagcccggct
     1801 gatcctcgat gccgcgccag atctgcgtat tccggcgacc atcagttttg tcgccagtgt
     1861 cgcccagttc acgccaaaaa ccgtcgaaac cagcgatgaa cggctgaaac tgatgttccg
     1921 cgtcaaagcg cgtatcccac cggaattact ccagcagcat ctggaatatg tcaaaaccgg
     1981 tttaccgggc gtagcgtggg tgcgggtgaa tgaagaactt ccgtggcctg acgacctcgt
     2041 ggtgaggttg ccgcaatgac gcatctggaa ctggttcccg tcccgcctgt cgcgcaactg
     2101 gcgggcgtga gccagcatta tggaaaaacc gttgcgctga acaatattac tctcgatatt
     2161 ccggcccgct gtatggtcgg gctgattggt ccggatggcg tcgggaagtc gagcttattg
     2221 tcgttgattt ccggtgcccg cgtcattgag cagggcaatg tgatggtgct gggcggcgat
     2281 atgcgcgacc cgaagcatcg ccgcgacgtc tgcccgcgca tcgcctggat gccgcagggg
     2341 ctgggcaaaa atctctacca caccttgtcg gtgtatgaaa acgtcgattt tttcgctcgc
     2401 ctgttcggtc acgacaaagc ggagcgggaa gtgcgaatta atgagctgct gaccagcacc
     2461 gggttagcac cgtttcgcga tcgtccggca gggaaactct ccggcgggat gaagcaaaaa
     2521 ctggggctgt gctgtgcgtt aatccacgac ccggaactgt tgattcttga tgagccaaca
     2581 acgggggttg actcgctctc ccgcgcccag ttctgggatc tgatcgacag tattcgccag
     2641 cggcagagca atatgagcgt gctggttgcc accgcctata tggaagaggc cgaacgcttc
     2701 gactggttgg tggcgatgaa tgccggagaa gtgctggcaa ccggcagcgc cgaagagttg
     2761 cggcagcaaa cgcaaagcgc cacgctggaa gaagcattta taaatctgtt accgcaagca
     2821 caacgccagg cgcatcaggc ggtagtgatc ccaccgtatc aacctgaaaa cgcagagatt
     2881 gccatcgaag cgcgcgatct gaccatgcgt tttggttcct tcgttgccgt tgatcacgtc
     2941 aatttccgca ttccacgcgg ggagattttt ggattccttg gttcgaacgg ctgcggtaaa
     3001 tccaccacca tgaaaatgct taccggactg ctgcccgcca gcgaaggtga ggcgtggctg
     3061 ttcgggcaac cggttgatcc aaaagatatc gatacccgcc gtcgggtagg ctatatgtcg
     3121 caggcgtttt cgctctataa cgaactcacc gtgcggcaaa accttgagtt acatgcccgt
     3181 ttgtttcaca tcccggaagc ggaaattccc gcgcgagtgg ctgaaatgag cgagcgtttt
     3241 aagctcaacg acgttgaaga tgttctgccg gagtcattgc cgctcggcat tcgccagcgg
     3301 ctttcgctgg cggtggcggt gattcatcgc ccggagatgt taatcctcga tgaacctact
     3361 tctggcgtcg atccggtggc gagggatatg ttctggcagt tgatggtcga tctctcgcgc
     3421 caggacaaag tgactatctt catctccacc cactttatga acgaagcgga acgttgcgac
     3481 cgcatctcac tgatgcacgc cggaaaagtg cttgccagcg gtacaccgca ggaactggtt
     3541 gagaaacgcg gagccgccag tctggaagag gcatttatcg cctatttgca ggaagcggca
     3601 gggcagagca acgaagccga agcgccgccc gtggtacacg acatcaccca cgcgccgcgt
     3661 cagggattta gcctgcgccg tctgtttagc tacagccgcc gcgaagcact ggaactgcgc
     3721 cgcgatccgg tccgttcgac gctggcgctg atgggaacgg tgatcctgat gctgataatg
     3781 ggttacggca tcagtatgga tgtggaaaac ctgcgctttg cggtgctcga ccgcgaccag
     3841 accgtcagta gccaggcgtg gacgctcaat ctctccggtt cccgttactt tatcgaacag
     3901 ccgccgctca ccagttatga cgagcttgat cgtcggatgc gtgcgggcga tatcacggtg
     3961 gcaattgaga tccctcccaa tttcgggcgc gatatcgcgc gtggtacgcc tgtggaactc
     4021 ggcgtctgga ttgacggagc gatgcccagt cgtgccgaaa cggtaaaagg ttacgtgcag
     4081 gcaatgcacc agagctggtt acaggatgtg gcgagccgac aatccacacc cgccagccaa
     4141 agcgggctga tgaacattga gacgcgttat cgctataacc cggacgtaaa aagcctgcca
     4201 gcgattgttc cggcggtgat cccgcttctg ctgatgatga tcccgtcaat gctaagcgcc
     4261 cttagcgtgg tgcgggaaaa agagcttggg tcgattatca acctttacgt gacccccacc
     4321 acgcgtagtg aatttttgct tggtaaacag ttgccataca tcgcgctggg gatgctgaac
     4381 tttttcctgc tctgcggcct gtcggtgttt gtgtttggcg taccgcataa aggcagtttc
     4441 ctgacgctca ccctggcggc gctgctgtat atcatcattg ccaccggaat ggggctgctg
     4501 atctccacct ttatgaaaag ccagattgcc gccattttcg gaacggcgat tatcacgttg
     4561 atcccggcga cacagttttc cgggatgatc gatccggtag cttcgctgga agggcctgga
     4621 cgttggatcg gcgaggttta cccgaccagt cattttctga ctatcgcccg cgggacgttc
     4681 tcgaaagcgc tggatctgac tgatttgtgg caacttttta tcccgttgct gatagccatc
     4741 ccgctggtga tgggcttaag tatcctgctg ctgaaaaaac aggagggatg atgcgccatt
     4801 tacgcaatat ttttaatctg ggtatcaaag agttgcgcag tctgctcggt gataaagcga
     4861 tgctgacgct gattgtcttc tcgtttacgg tgtcggtgta ttcgtcagcg accgttacgc
     4921 caggatcgtt gaacctcgcg ccgatcgcca ttgccgatat ggatcaatcg cagttatcga
     4981 accggatcgt taacagcttc tatcgtccgt ggtttttgcc accggagatg atcaccgccg
     5041 atgagatgga tgccggactg gacgccggac gctatacctt cgcgataaat attccgccta
     5101 attttcagcg tgatgtcctc gccggacgcc agccagatat tcaggtgaac gtcgatgcca
     5161 cgcgcatgag ccaggcattt accggcaatg ggtatatcca gaatattatc aacggtgaag
     5221 tgaacagctt tgtcgcgcgc taccgtgata acagcgaacc gttggtatcg ctggaaaccc
     5281 ggatgcgctt taacccgaac cttgatcccg cgtggtttgg cggggtgatg gcaatcatca
     5341 acaacattac catgctggcg attgtattga ccggatcggc gctgatccgc gagcgtgaac
     5401 acggcacggt ggaacacttg ttggtgatgc cgataacgcc gtttgagatc atgatggcaa
     5461 aggtctggtc gatggggctg gtggtgctgg tggtgtcggg attatcactg gtgctgatgg
     5521 tgaaaggcgt gctgggtgta ccgattgaag gctcgatccc gctgtttatg ctgggcgtgg
     5581 cgctcagtct gtttgccacc acgtcaatcg gcatttttat ggggacgata gcgcgttcaa
     5641 tgccgcaact ggggctgctg gtgattctgg tgctgctgcc gctgcaaatg ctttccggtg
     5701 gatccacgcc gcgcgaaagt atgccgcaga tggtgcagga cattatgctg accatgccga
     5761 cgacacactt tgttagcctc gcgcaggcca tcctctaccg gggtgccgga ttcgaaatcg
     5821 tctggccgca gttcctgacg ctgatggcaa ttggcggcgc gtttttcacc attgcactgc
     5881 tgcgattcag gaagacgatt gggacaatgg cgtaaataca attgcccgat gcgtaagctt
     5941 atagggacta cgcatcggtg caatttattg aattttagtt gtttttaggc cggataaggc
     6001 gtttatgaac tgacccccaa aacctggaca gtttcagtta accggctttt tgatactcag
     6061 tcctgtattc agggcaagat tacgaaagcc c
//
LOCUS       DAZGJW010000222         6076 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZGJW010000222 DAZGJW010000000
VERSION     DAZGJW010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.224"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..522)
                     /locus_tag="ACHKIK_004697"
                     /pseudo
     CDS             complement(1..522)
                     /locus_tag="ACHKIK_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(527..691)
                     /locus_tag="ACHKIK_004698"
     CDS             complement(527..691)
                     /locus_tag="ACHKIK_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085713.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            complement(688..1421)
                     /locus_tag="ACHKIK_004699"
                     /pseudo
     CDS             complement(688..1421)
                     /locus_tag="ACHKIK_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(1455..1916)
                     /locus_tag="ACHKIK_004700"
     CDS             complement(1455..1916)
                     /locus_tag="ACHKIK_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO9085714.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            complement(1909..2949)
                     /locus_tag="ACHKIK_004701"
     CDS             complement(1909..2949)
                     /locus_tag="ACHKIK_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO9085715.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            complement(2921..3472)
                     /locus_tag="ACHKIK_004702"
     CDS             complement(2921..3472)
                     /locus_tag="ACHKIK_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO9085716.1"
                     /translation="MKITTEQVCEALDTCVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGSYEYRCTTMG"
     gene            complement(3456..3683)
                     /locus_tag="ACHKIK_004703"
     CDS             complement(3456..3683)
                     /locus_tag="ACHKIK_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO9085717.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            3760..4167
                     /locus_tag="ACHKIK_004704"
     CDS             3760..4167
                     /locus_tag="ACHKIK_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9085718.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            4432..4731
                     /locus_tag="ACHKIK_004705"
     CDS             4432..4731
                     /locus_tag="ACHKIK_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085719.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            4804..5022
                     /gene="ydfC"
                     /locus_tag="ACHKIK_004706"
     CDS             4804..5022
                     /gene="ydfC"
                     /locus_tag="ACHKIK_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO9085720.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            5045..5452
                     /locus_tag="ACHKIK_004707"
     CDS             5045..5452
                     /locus_tag="ACHKIK_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085721.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(5430..5663)
                     /locus_tag="ACHKIK_004708"
     CDS             complement(5430..5663)
                     /locus_tag="ACHKIK_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085722.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
BASE COUNT         1497 a         1582 c         1345 g         1652 t
ORIGIN      
        1 tacatttttg taatcaacat cctgtttgga atagccagcc tttaatggat aactatttct
       61 gacaatgcaa tgagtataat caagtccatc ttccactgag aattagaggc ggcatgcttt
      121 ttccggctct tgccggatat ccgtaattgt ccataatctg cagatttata ccttctgcat
      181 gacctgtcag cgaaaatttg tccggtgttt ctacgggaat gacatcaaaa gttacacgca
      241 ctcgcgttac cgtgtagacc tactttcctg cacttgcaag atcacagtgg tgtaaccgta
      301 acaggaattt attctctgga ccggcagtaa atccctgagt ggcgtggttc ccatatcaat
      361 ttcccgccag gcagcctcca ttgccagcgt acaggctgga gccatgacct gccctttaaa
      421 tctggcccga ccatcccacc ggacgtgttc ttctcccctg aacttaggta cagtcatctc
      481 cagtggcaca aaagtgtcag cgccataatt tttgaccgtt atcgcgctac ggatattttg
      541 ttgactggtg aaaatcaccc cgcagaatca ggcttattcc cttaacctgg gctttcatcc
      601 tgaccgccgc ctcactacga ccaatcagac tgccgatgca ttttaccttc attgttagta
      661 tcatgatttc aggcctgcac catccgctca ttgcccggac ttccgacaaa tcccggcaac
      721 catatcccgg tgcttgttca gctcccgcag cgcggcgcag actcgctccc acttctggac
      781 atgattcttc gccctacgca gttcgcgatt tgccatatgc agcgatggca aaatcaaatc
      841 atcttctcgc gttgcagtaa acgatggcag cgactgcaca atgtccgcca cagtttctgt
      901 tttaatatct tcctgtgttg catctttctg taccggtaac gaacaccggc tggctgagga
      961 aaggctttac catcggtttc cgctaccgat tcaactttcg gctctgctgg taaattatca
     1021 cctggtatgc agtaacgaat tttaccgttc tggtttacgc gaatcagacg acctttgctg
     1081 attgccattg ccagcgttga agcaactttg cgggatgttg taccgaacag cgtagccagt
     1141 tcatccgccg tttgtggtcc gcgttgttca atcgtcgcgg ttaaatcgca ctctgagatt
     1201 ttcgcttctg ttgccgtggt ggtttcttct ggcgctggct gttcctgctg aacgttgtta
     1261 tcagccacac gccaggtgta tacgctttta tcaacgaagc cagccttttt cagttcccac
     1321 agctcgttca gtacttcttc acgactgata tcaagtcgcg cagccagctc taccgacgtg
     1381 gcttttccca tcgctttcag tgcgtcaaaa acagtctcca taaatttcct cccggtaaaa
     1441 aatcacttct caactcaaac aaaaccagcc gctttccggc gttcatattc ctgtttcagc
     1501 aactcaattg gcgttggccc cgacgggcgt ttgggggccg ccagttgtcg ccggactggc
     1561 ggaacgctca ggccgttact aacatgcttt gcccatttcg tcagttgccg ttctgcaagc
     1621 cgttttaatt ccccttcggt catctggcgc tcaatcccct ttgaacgcat ctcgaggcaa
     1681 atgtgataca gcacaggctg agaccacggg tacttatcac ttccgtcgta tcgccaggac
     1741 tcattgcgcc agcggcggta ctcctccatc acagcatcca ccgtcagacc gaatggattg
     1801 gctccgcttt tcgaaatcag tgccacaaac tcagccaggt ccggaggcca tgtttcaccc
     1861 gcccggcagc ggtccatgca ctggcggcag acctgccgga tttgctgctc agtcatcgcg
     1921 ccaatctgtg caatccagag cttcgaaggt gcggccccgt tcttctggat ccagcggttc
     1981 gaataaacca cccccatgag ttcccacagc ttccaggccg tttccgtcgc tgataaatcc
     2041 gttttcacgt tcccactgct cacgtgctgc ccgaatttcc tgaactgccc gtgatgcggt
     2101 gccacctggt gctgctgcat ggtttacccc tttgctgact ggtttaacct gcgccctgac
     2161 gtgatttacg tgacgggcga atttctgctc ccactgaatc tgcgtaaaca ctttcccctc
     2221 cgctgcccag tagtcccgga aggcggcaag ttcagcaggt gtaaattctg tctccggcaa
     2281 agccatcccc cacaacgcag cccgtcgtcg aaaatcccgt gacggatacc agctatcgct
     2341 catcgggaat tttccgatgg gttcgctcag gccatacagg aatacagggg gggctgcctg
     2401 taacgacaaa acttcctgct cactggtcgg agcactctcg cgtgcgttat gtgtggggtt
     2461 tagatctttg ggttcctttg ggttccgtga tccgtttttg ggtgtctttg atggaaaatt
     2521 tgggtgtctt tggttatttt ccatgcagct aagagttctg tttttgggtc tgttttgtgc
     2581 tgaaacataa ccattttcgg tactgttttt attaacagca ccaattttac ccacctttaa
     2641 agactcccgt ttttgggtgt attcaggctc ggcaacactt tcttctacac cgataagtcg
     2701 gtacaccaca atttgctttg ttctgccttt tctctcaccg gtatcaacaa ttaacccaat
     2761 ctccatcagg tgtcgtaagc tgtcctgcac agtctttttg ttcagttccg ttacttctgc
     2821 cagtgcagat acagacgggt atgcacacaa atcggcaccg cacatatcag caagccaggt
     2881 caatactgac ttactggatg aactgccggt tttcaccttt ttagcccatc gtagtgcatc
     2941 gatactcata cgaacccctg gcagacattt gtttatctgc aaagtaatat tggtattgct
     3001 gacgatacgc gtgcttgaaa gcaatagctt tttctataag ctcgtcagtc tcacgttcca
     3061 caacaactgg atccgcaaaa agcagcccgg actccaccac atcgccatat tctttgttta
     3121 acccggcgat catgtacgta atgctttttc cgtcagtaat ttcacaatac aatctgaaat
     3181 cgctgatccg gatagcctcc ataattgccg gaatcagcgc cgtgaatttg tcacgcttat
     3241 ctctggtgtc gatagctttc cagcgttcga atatcttcac ccggttaacg cccagcgccc
     3301 gttgatcaac ctcgccatca ttaaacgtga cgcgttgaac atcgatgttc gggcgttctt
     3361 tcagagccca gaatgcttcc gtgattaata tcgtcgcctg ctcctgtgtc attcctggtc
     3421 ggcatacaca ggtatccaga gcctcacaaa cctgttcagt ggtgattttc attgttcaac
     3481 cgccccgccc gctttgcctt acgatattcg tcataaactt tggggtcgta ctgaagttcc
     3541 ccgccggatg cctcttgcag gcgcatcgcg cgaccttcag gaaccagttc cccccatgca
     3601 gcaacgcttg ccagcctaac tcctgcgaca ttggcaagct ttgttttgct gccaaaaaac
     3661 gctatagcat caattttcaa catatcgaac cccttagatt ttcctaagga aactagatcg
     3721 tagagaaacc taagtcaaga aaaattagaa ttacctaata tgaaaaacga aaccttcggt
     3781 gctcgcctct tatacaggcg aaaaaaatta aaactgtctc aggccgcatt aggtaagctg
     3841 gtcaaagtgg ctcacgtaac aatttctcaa tgggaaagag atgaaacaca gccagcgggg
     3901 aagagattat tcgcactgag ccaggcgctt cagtgctcgc cgacttggct tctttttgga
     3961 gatgaagata agcaaccagg cgaaccgatc ccagataatc agccagtcaa tctgacagaa
     4021 gatcaaaaag agttgcttca actgttcgac gcactgcctg agtcagagca aaaggctctg
     4081 ttgtcagaga tgcgtgctcg agttgagaat ttcaacaaac tttttgaaga actactcaaa
     4141 gctcgcaaaa gaagcgcaaa taaataaccc cccttttttt cgcaactctc tgtaataaaa
     4201 agcacaaact ttcaaatact tgtgtttttt acatcaagaa gcttaggttt ttctacacaa
     4261 aaagcttgac cataactctt aggctattct aaattctact catcaagaca ccgcacggtg
     4321 ttctcagcaa acagttccgc tacccggcgt taaggggaag cagaggattt ctcagtgggc
     4381 gaagtcaaac atcagaatgg aaggcgtcca gggatcagca aagaaacagc gatggcgctt
     4441 tatattgata tcagcgccat tgccggacag gtaagagtta tcagagcggt aactaagcgg
     4501 tatgcgcctt tacttcagaa agtctctggt gagtgcaccg aagatattgt caacgatttc
     4561 gtcatcgaac tgcgaggact catcttcagt tacaaggtga ccacaatttt tgcagatggc
     4621 tcccgcgaaa ctgtcagagc cctgcggctt aaaggatgtg tcaaagactt agccaccaca
     4681 ttctgggcaa gaaaacttga ttgtattcat aaccaatttc ctctcgagta acagacccct
     4741 cagaggatac cacctcgcct gacgtggtta aaaagcaggc aacgctaacc acaaggagcc
     4801 gacatgcaga aacgagaccc cgtcatcatc gcgccagact ataccgatga tgaactttat
     4861 gagtggatgc accagaaaat taaggcggtg caggacctga aatgggccaa tgaagccagg
     4921 gctaagcagg ctgaaaatct gtccgctctg gagcaggata tcaccaatct ggaaaaagca
     4981 gcggcattaa gcattgccag aatgattaca tacccacgtt aatagctaac caacgaggct
     5041 aataatggaa tttaaagatt taccaacgcc attacaggaa atggcatcga atatagttcg
     5101 ttcacaactg gctactcttg accttagtac cgcagaaaaa gaaaccatcg ataatatggt
     5161 tcgtaatgtg cgcaatgctt tttctgggct atatggttct gataatcaaa agcaggaaag
     5221 cgatgttaat aaacgggtaa tttctgtttg cgtgaatggc catgttcttt catcaatcaa
     5281 aacagaaacg gcgacagtct tcgattgcct ttgcattgta cagagccttg ttgatgccct
     5341 gtttcgttca gtgaatttag aaaatgatgc aaatctgcga gggcgcataa tagcacatcc
     5401 atatgcacat actttaggct ctgtggatat caaagatccc acaaatcttt aatgaaatag
     5461 ttaacgcgaa ttgtacttgc tctttcggtt gctttcagaa tacgcgttga aactgctggc
     5521 ggtaatttgg tattccattt attaaaatca tgcccgggaa agtactcttc gaaaatactt
     5581 ttaactgcag actcgcctat tgaaatgctg cttaccatgc gattttgata aaggcattta
     5641 gcaataagag ttgattttaa cattcaccct cctgagggtt ggtaattaag gagttctcca
     5701 cgggtgaagt ggagtgcgtg cgccggacac gggtgaacat ccggcactga cagtttactg
     5761 aaaggatatt tctctgaaaa gtcagaccat aacgcgaaag cgcacggcga ggtagctggt
     5821 tcatagatag cctgtcgtta aattttcgtc gaccgtgcgc ttccggttgt ggcaatccgc
     5881 gaaatggcgc ggcggtaagt atggaggggt tattccttcc ccgttgagga caccgggttg
     5941 tcaggttgac catacgctta agtgacaacc ccgctgcaac gccctctgtt atcaattttc
     6001 tggtgacgtt tggcggtatc agttttactc cgtgactgct ctgccgccct ttttaaagtg
     6061 aattttgtga tgtggt
//
LOCUS       DAZGJW010000223         6063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZGJW010000223 DAZGJW010000000
VERSION     DAZGJW010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.225"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..932)
                     /locus_tag="ACHKIK_004709"
     CDS             complement(<1..932)
                     /locus_tag="ACHKIK_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFO9085723.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFSYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYA"
     gene            1153..1581
                     /gene="uspG"
                     /locus_tag="ACHKIK_004710"
     CDS             1153..1581
                     /gene="uspG"
                     /locus_tag="ACHKIK_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO9085724.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(1702..3267)
                     /gene="ahpF"
                     /locus_tag="ACHKIK_004711"
     CDS             complement(1702..3267)
                     /gene="ahpF"
                     /locus_tag="ACHKIK_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO9085725.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3438..4001)
                     /gene="ahpC"
                     /locus_tag="ACHKIK_004712"
     CDS             complement(3438..4001)
                     /gene="ahpC"
                     /locus_tag="ACHKIK_004712"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO9085726.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            4373..5119
                     /gene="dsbG"
                     /locus_tag="ACHKIK_004713"
     CDS             4373..5119
                     /gene="dsbG"
                     /locus_tag="ACHKIK_004713"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO9085727.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            5328..>6063
                     /locus_tag="ACHKIK_004714"
     CDS             5328..>6063
                     /locus_tag="ACHKIK_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9085728.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLG"
BASE COUNT         1592 a         1494 c         1416 g         1561 t
ORIGIN      
        1 gcgtagacgc ccggtacgct aacaatgccg ccacgcctga ccgccgcaat acactggcgc
       61 aacgctttac cgctgctgcc ctccagtttc aggttagtca gcaccgtttc cgtggtgctg
      121 cctttcgctt caaaaccgac ggcgtctatt actgcatcca cgccccggtg acctgccgtt
      181 tgttcaataa ttgactgtgc cggatcgcta tcttcatcga aattaatcgg gatcgcgccg
      241 tagcggtcag cggcgaaacg caagcggtag ggatgatgat caacaacaaa aatctgttcc
      301 gcaccgagca accgtgcaca ggcgattgtc aacaatccca ctggaccggc accatagact
      361 gcaacgcttg aaccttgttg gatctgcgca ttttttgctg cctgccatgc cgttggcaga
      421 atatcagaaa ggaaaagggc tttatcatct gaaagcaaag gcggtacttt aaacggcccc
      481 acattccctt tagggacgcg gacatattcc gcctgtccac caggaacgcc accatacagg
      541 tgactataac taaacaatgc cgcgggagct ggtatctgtt ttttattgag cgcagcgcct
      601 ttacccgcat tggtattttc gcaggcggca tattgctgca ttcgacagaa aaaacagtcg
      661 ccacaagcaa tgacaaacgg aattaccacc cggtcgcctt tttgcaaatt ttttacgtcc
      721 tttccggttt caacgacttc ccccataaat tcatgaccaa aaatatcgcc atgtttaacc
      781 tggggtattt tgcctcgata aagatggagg tcagagccac agatcgccgt tgccgtaata
      841 cgcagaataa tatcatctgc ctgttcaatg cccggatcgg gaacattttc tacctgaacg
      901 tgatgtgggc cgtgataagt caatgctttc atgcaacctc cagagacagt tcaaaaaaga
      961 gtcagtcttt aagggtagct atcacagcgg gaaaacgggc ggtaccgcag gttttgggag
     1021 aattcctggt gttcagtgag aatatattaa tcacagaaac cgttcgtaag gaaaaagaac
     1081 cagacaaata tcagggataa atattataat catggcgaat gatttgttca tgattaacag
     1141 ggagaaaggg ttatgtataa gacaatcatt atgccagttg atgtatttga aatggaattg
     1201 agcgacaaag ctgttcgcca cgctgaattc ctcgcccagg atgacggagt tattcatcta
     1261 cttcacgtac tacccgggtc agccagcctg agcctgcacc gttttgccgc tgatgtgcgt
     1321 cgttttgaag agcatctgca acatgaagca gaagaacgtc tgcaaacgat ggtcagccac
     1381 ttcaccatcg atccttcccg cattaaacaa catgtccgtt ttggtagcgt gcgggatgaa
     1441 gtcaatgagt tggcagaaga actgggggct gatgttgtag ttattggttc tcgcaaccca
     1501 tcgatttcga cccatctgtt aggttctaac gcctcgagcg tgatccgcca tgccaatctg
     1561 ccggtgctgg ttgtgcgtta attgccagaa taaatatccc gccctgcccg gagccatccc
     1621 ggcagggctt tttaatgcca taaaaaagcc gccaggtttg accccggcgg ctaagcaatt
     1681 gcaggtgaat cttacttctt cttatgcagt tttggtgcga atcaggtagt caaaagcact
     1741 cagagaggct ttggcacctt cgcctgtggc gatgataatc tgcttgtacg gaaccgtcgt
     1801 acagtcaccc gctgcgaaca cgcctttgac gttggtttcg catttcgcat caatgataat
     1861 ctcacccatg cggttacgtt cgactgcgcc ttccagccag ttggtgttcg gcagcagacc
     1921 aatctggacg aaaataccgg ccagttcgat gttgtgaata tcgccgctga cacgatctcg
     1981 atattccaga ccaacgactt tgctgccgtc gcctttcact tccgtggttt gcgcattcag
     2041 aataatgtcg acgtttttca ggctgcgcag tttgtcctgc agaacctggt cggctttcat
     2101 ttctggcgca aattcgagca gcgttacgtg ctcaacgata cccgccaggt caattgccgc
     2161 ttccacgccg gagttaccgc cgccgataac cgctacgcgt ttgcctttaa acagcgggcc
     2221 gtcgcagtgc gggcagtagg tcacgccttt ggtgcgatac tgatcttcgc caggaacgtt
     2281 catgttgcgc cattttgcac cggtcgccac gataatgctg cgtgctttca gtaccgcgcc
     2341 agaagctgtt tcaatctgat gcagaccacc ttcaactgct gctgggatca gtttgctggc
     2401 gctctggctg tcgatcacat caacgtcgta ttcatcaacg tgaactttca gtgcgcctgc
     2461 cagcttctgc ccttcagtct tcggtacaga aatgtagttt tcgatatcaa cggtatcgag
     2521 gatctgacca ccaaaacgtt cgcccatcag accggtacgg atgcctttac gtgcggagta
     2581 aattgctgca gctgcacccg ccgggccgga accaacgatt aatacgtcat aagcatcacg
     2641 cttgttcagc tcttctgccg cacgtttttc cgcgcccgta tcaattttgg caacgatttc
     2701 agtcaacgtc atgcggccct gaccaaactc tttcccgttt acgaacactg ccggaacgcc
     2761 catcacgttg cgatcggtga tttcgttctg gaaggtgccg ccgtcaattg cagtgtgctt
     2821 gatgcgcggg ttcagtacgc tcatcaggtt cagcgcctgc accacgtccg ggcagttatg
     2881 gcaagagagc gagtaatagg tttcgaattc aaaatcaccg tcaatatggc gaatctgctc
     2941 cagcagagac tgcgcttctt tcgacggatg accaccggtc catagcaacg ccagtaccag
     3001 cgaggtgaac tcgtggccca gcggggagcc tgcaaaacgt ggcccctggt tggaacctgg
     3061 gttggtgatc aggaaagacg gcttacgcac cggcaagctg ttatcttctt taaaggtgac
     3121 tttgtctgac agttctgcga tttcagccaa cagttccttg atttctgccg atttagcgct
     3181 gtcatccagc gtggcaatta actcaacagg cttggtcaat ttctcaaggt aagccttgag
     3241 ttgagttttc atatttgtgt cgagcatgtt tatctcctgg gctttaaaca tcatcatgca
     3301 agcggcctct gcggctacct ggatgcagct tgcatcatgg tgcggggaag aaaatcgggt
     3361 gccgcagcac ccgaagaatt aggtgaattt ccgaagtatt tcgcgccgga gttggtagcg
     3421 cgaaagacga aggaaattta gattttacca accaggtcca gagacggagc cagagttgct
     3481 tcaccttctt tccatttagc cgggcaaact tcacctgggt gagaagctac gtactgtgct
     3541 gctttgattt tacgcagcag gtcagacgcg tcacggccaa tgccttcagc ggtaacttcg
     3601 attgcctgga tgataccctg cgggtcaaca acgaaggtcg cacggtcagc cagaccttca
     3661 tcttcacgca tgttgtcgaa gttacgggtc agggcgccag tcgggtcgcc gatcatcgca
     3721 tatttgattt tagcgatggt ttcagagctg ctgtgccatg ctttgtgggt gaagtgagta
     3781 tcggtagata ctgcgtatac gtctacgccc agtttctgca gttcttcgta gtggtcagca
     3841 acgtcaccca gttcggtcgg gcatacgaaa gtaaagtcag ccgggtagaa gaagaagacg
     3901 ctccagcggc cttcggtatc tttttcggtg atttcgatga attcgccgtt tttgaatgcc
     3961 tggtttttaa aaggtttaat tttggtgtta atcaaggaca tctatacttc ctccgtgttt
     4021 tcgatgagat gtaaggtaac caatttttgc cgattcggct aatgcgtttc cattatcaaa
     4081 tcgataagtt ttaccttaca accttcgtaa gacaactttg tgaactttgc accgccagcg
     4141 gcgataaaaa gtaaaatggc cccctttcca ggtgaccgtg taatctgaac taccctttgg
     4201 tatcttcagt gcctgcctgc aggctgaggt gttgccctta caaatgcaac aacgacatgg
     4261 attacaacac cctcataaac aaagggcaat cacctgatct aagctcttac ctatgacagt
     4321 gataggttat gccttttact cgacttttgc actgactgaa aaggacaaat taatgttaaa
     4381 aaagatactt ttactggctc tgcttcctgc aatcgccttc gcagaggaac ttcctgctcc
     4441 agtaaaagcg attgaaaaac agggcattac aatcatcaaa acattcgatg cccccggagg
     4501 aatgaaaggt tatctcggaa agtatcagga tatgggcgtc accatctacc tgactccaga
     4561 tggtaagcac gctatctctg gttacatgta caacgagaaa ggtgaaaacc tgagtaacac
     4621 acttatcgaa aaagaaattt acgcaccagc cggacgcgaa atgtggcaac ggatggaaca
     4681 atcccactgg ctcctcgacg gtaaaaaaga tgcgccggtc attgtctacg tgttcgccga
     4741 tccgttctgc ccatattgta aacagttctg gcagcaggcg cgcccgtggg tagattctgg
     4801 caaagtgcaa ttaagaacat tgttggttgg ggttatcaag ccagaaagcc cggcgacagc
     4861 agcggcaatt cttgcctcca aagatcccgc aaaaacctgg caagaatatg aatcctctgg
     4921 tggcaagctt aagctaaacg tgcctgcaaa cgtaagtaca gaacaaatga aagtgttaag
     4981 cgccaacgag aaactcatgg acgatctggg ggcaaatgtc acaccagcga tctattacat
     5041 gagtaaggaa aatacgctgc aacaggccgt ggggttgccc gatcagaaaa cgcttaatat
     5101 cattatgggg aataaataaa agataattca tggatggttg aaatacatcc ctgcttttca
     5161 accatcctaa aaaagcatga attgctcgct ctgccctaag tggctatgct attttagaaa
     5221 aacttaatcc tgagttaagc aaaaaaatat aatcaataaa aatatatacg caaaaaacat
     5281 tgattaagtg aatataccat ggaagaaaaa tataaccgga gtagtgtatg gccaatctct
     5341 acgacttgaa aaagttcgac ttaaaccttc ttgtcatatt tgagtgtatt taccagcatt
     5401 taagcattag caaagctgct gaatcactat atatcacccc atcagccgtg agccagtcgt
     5461 tacaacgctt acgtgcgcaa tttaatgacc ctttattcat tcgctcagga aaaggtatcg
     5521 cgccgacgac aacagggttg aatctgcatc accatcttga gaaaaacctc aaaggccttg
     5581 aacaaacaat taatatagtc aataagtccg aactaaagaa aaactttatt atttacggcc
     5641 ctcagctcat ttcttgctct aataatagta tgctgatccg ctgcctacgg caagatgcct
     5701 ctgtcgagat tgagtgccat gatatcctca tgtcagcaga gaatgctgaa gaactgttgg
     5761 ttcaccgcaa agccgatctg gtgatcaccc aaatgccagt gatcagccgt tccgtcattt
     5821 gcatgccatt gcatacaatt cgcaatactc taatttgtag taataagcat ccgcgaatta
     5881 ctgacaatag cacttatgaa caaattatgg cagaagagtt cacccaatta atatccaaat
     5941 cagcaggagt tgatgatatt caaatggaaa tagacgagaa gtttatgaat cgcaaaattt
     6001 catttcgtgg ttcctctttg ctgacaatca ttaatagcat tgcagttaca gatttactgg
     6061 gaa
//
LOCUS       DAZGJW010000224         6012 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZGJW010000224 DAZGJW010000000
VERSION     DAZGJW010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.226"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(26..178)
                     /gene="hokA"
                     /locus_tag="ACHKIK_004715"
     CDS             complement(26..178)
                     /gene="hokA"
                     /locus_tag="ACHKIK_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO9085729.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(503..2572)
                     /gene="glyS"
                     /locus_tag="ACHKIK_004716"
     CDS             complement(503..2572)
                     /gene="glyS"
                     /locus_tag="ACHKIK_004716"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO9085730.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2582..3493)
                     /gene="glyQ"
                     /locus_tag="ACHKIK_004717"
     CDS             complement(2582..3493)
                     /gene="glyQ"
                     /locus_tag="ACHKIK_004717"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO9085731.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3588..3887)
                     /locus_tag="ACHKIK_004718"
     CDS             complement(3588..3887)
                     /locus_tag="ACHKIK_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO9085732.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLSMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            4062..5057
                     /gene="wecH"
                     /locus_tag="ACHKIK_004719"
     CDS             4062..5057
                     /gene="wecH"
                     /locus_tag="ACHKIK_004719"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO9085733.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(5099..5536)
                     /gene="yiaA"
                     /locus_tag="ACHKIK_004720"
     CDS             complement(5099..5536)
                     /gene="yiaA"
                     /locus_tag="ACHKIK_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO9085734.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(5582..5923)
                     /gene="yiaB"
                     /locus_tag="ACHKIK_004721"
     CDS             complement(5582..5923)
                     /gene="yiaB"
                     /locus_tag="ACHKIK_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO9085735.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
BASE COUNT         1496 a         1526 c         1443 g         1547 t
ORIGIN      
        1 gggaataact tcccgccgaa gactcttact cttttaaatt gcaggctaaa aatgccgcca
       61 gctcataact cccctgttta atatgcaatt cacacagtga atctcttatc atccaggtga
      121 aaaataaaag cgtgaaacaa atcactatta aagaaagtaa tctatatttc tgcggcattc
      181 ccagccccct gtgttgattt ccaacgagta agaggctaac cttatggtgt tcaggcatag
      241 ggtagcctca ctttgattta tagtcaggtg gggcttttct ctgtctgcct ttcggtgaat
      301 acctgagaca aacagtctca agcacccgtg gctattctag cttaataagt ttgtttcttc
      361 tccttgatat aatcctaaaa aacctcataa aattaatata tgagataatc tttattcagc
      421 agaagattat taaaggttgc tgtattattt agcgataata aaaaaagcct gccagatggc
      481 aggcttttta ataacggcgt tattactgca acagcgaaat atccgcaacg cgcaggaaca
      541 gttcgcgcag tttctccagc atggtcagac ggttgagacg caattctttg tcatcaacca
      601 tgaccatcac tttatcgaag aaagcatcaa ccggctcacg cagctctgcc agttcgacca
      661 gcgcatcctg gtaacgacct tccgcaaagt acggctccag cttgtcacgt agcaccacaa
      721 cctgcatcgc cagtttaatt tcttccggct ctttcagggt agaggcattc acgcggtcgc
      781 tcagcacttc gtcagatttc gccaaaatgt tggagacacg cttgttcgcc gccgccagtg
      841 cagcagctgc ttcaagggta cggaagtgcg ataccgcttt catgcgggca tcgaaatcag
      901 ccggacgagt cggacgacgc gccagtaccg cctggatggt gtcaacggtg taaccttcgt
      961 cctgatacca ggcgcggaag cgaccgagca taaagtcgat aacatcatca accacgttgg
     1021 cgttagtcag cttatcgcca tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa
     1081 ggttgaggtt cttctcaacg ataattcgca gcacgccaag tgcggcacga cgcagcgcaa
     1141 acgggtcttt gtcgcctttc ggatgctggc cgataccgaa gatacccgcc agagtatcca
     1201 tcttgtcagc aatcgccagc gcacaggcta ccgggttaga cggcaggtca tcaccggcaa
     1261 agcgcggctg atactgctca ttcagcgcca ccgcgacatc ttccgcttcg ccatcgtgac
     1321 gcgcatagtg catccccata acgccctggg tgtcggtgaa ctcgaagacc atgttggtca
     1381 tcaggtcaca cttagacagc aggcccgcac gggttgcgtg gttaacgtca gcgccaatct
     1441 gttcagcaat ccagccagcc agcgcctgga tgcggtcagt tttgtcgcgc agcgtaccca
     1501 gctgttgctg gaacagcacg gtttgcaggc gcggcaggtt atcttccaga cgttttttac
     1561 ggtcggtatt gaagaagaac tcggcatccg ccagacgtgg acgaacgact ttctcgttac
     1621 cagagataat ttgctgcgga tctttcgatt cgatgttggc aacgaagatg aagttcggca
     1681 gcagtttgcc atcgttcgca tacaccggga agtatttctg gtcacctttc atggtgtaaa
     1741 ccagggcttc agccggcacc gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg
     1801 gccactccac cagcgaagcc acttcttcca gcaggctttc gcttaagtca gcgttaccgc
     1861 caatcttacg cgctgcttct tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat
     1921 cggcgatgac tttcccgcgc tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg
     1981 tgaattccgg ttcgcccata aagcggtggc cgcgaatcac gcgatcggac tgaatgccca
     2041 gaatggttgc cggaatgact ttgtcgccca gcagcagggt cacggtgtgc accggacgca
     2101 cgaagtgcac gtcgcttgcg ccccaacgca tcagtttcgg gatcggcagt ttcgccagag
     2161 aagtcgcaac catattcggc agcagtgctt cggtgctttc gcccttcaca tgggcgcgat
     2221 acagcagcca ttcgccttta tcggtagtca gacgctcagc ctggtcaacg gtaataccgc
     2281 aaccacgcgc ccaaccttct gccgctttgc tcggtttgcc ttcagcgtcg aacgcctggg
     2341 caatcgccgg gccgcgtttt tcgatttcac gatccggttg cgcttccgcc aggttagcta
     2401 ctttcagcgc cagacgacgc ggagcagcaa accattgaac ggtgccgtgt gcgaggccag
     2461 cgttatccag ctccgcagta aaattcgcag caaaggactc agccaggctg cgcagtgctt
     2521 ttggtggcag ctcttcagtg ccgatttcca ccagaaaagt tttctcagac atagccgcct
     2581 cttacttatc tttgttgcac atcgggaagc cgagggcttc acgggaagcg tagtatgctt
     2641 ctgccactgc tttggtcagg gtgcgaatgc gcagaatgta gcgctgacgc tcggtgacgg
     2701 agatggcttt acgcgcatcc agcaggttga agctgtgggc ggctttcaga atacgctcgt
     2761 aggctggcag cggcagcgga ttttccagcg ccagcagctg ctgcgcttct ttctcgtact
     2821 gctcaaagca ggtgaacagg aagtccacat ccgcgtattc gaagttgtaa gtggactgct
     2881 ccacttcgtt ctgatggaac acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc
     2941 agaccaggtc gtaaacgctg tctacgccct gaatgtacat ggccagacgt tccagaccgt
     3001 aggtgatctc gccggtaacc ggtttacact ccagaccacc aacctgctgg aagtaagtga
     3061 actgcgtcac ttccatgccg tttagccaca cttcccagcc cagtccccag gcacccagag
     3121 tcgggttttc ccagttatct tccacgaaac ggatgtcgtg aatagtcggg tccatgccca
     3181 gctctttcag agaaccgagg tacagctcct gaatattgtc cggcgatggc ttaatgacca
     3241 cctggaactg atagtagtgc tgtaaacggt tggggttttc gccgtagcga ccatcggtcg
     3301 ggcgacgaga aggctgaaca taagcagccg ccatcggttc tggccccagc gcgcgcagac
     3361 aggtcattgg gtgagaggtt cccgcgccga cttccatgtc caatggttga acaatggtgc
     3421 agccctggcg agcccagtaa tcctgtaagg tcaggatcaa gccctggaag gtcctggtat
     3481 caaacttttg catattattt cgtgctggat acgtgtggat ttaaaggaag ggatcagtat
     3541 acccgctgga tggaagatat acagtacgaa acgggaaaaa gcagggctta acgcatggaa
     3601 agatgcaaaa aatggccatc tgcgtcaaaa gaacagacaa actgctcttt acggaaggta
     3661 taaccgcgca tttcatagct tccctggaac tgttcgaagg cggtgacgtc gattttttgc
     3721 tcgccggtgt tataacgttt tgccgcctga tccttgcata acgcttccat actcaacgaa
     3781 cgcagaggat caacctttac ccgttgtgct ttctgcacgg gtgacggtgt agaacaaccc
     3841 actagcacca taagcgccat tgccggaaag aaagcgttca tcatcattaa attaccgtct
     3901 ggtttgcagg tgagtcttat tatttatatc ggtatagtgg ctgatatctt acgaatttcc
     3961 ttaagcctct ataagctgta gtgataaaac tcagattatt ccagtgcgcc aaccaaatga
     4021 tttatcggtc agactttttt taaccagata cagagacaca gatgcagccc aaaatttact
     4081 ggattgataa cctgcgaggg atagcgtgtt taatggtggt gatgattcac accactacct
     4141 ggtatgtgac caatgctcat agtgttagcc ccgtcacatg ggatatcgcc aatgttctga
     4201 attctgcctc tcgtgtcagc gtgccgctat ttttcatgat ttccggctat ctcttttttg
     4261 gcgaacgcag cgcccagccg cgccatttct tgcgtatcgg cttatgtctg attttttata
     4321 gcgcaatcgc actgctctac attgcgctgt ttacctccat caatatggag ttagcgctga
     4381 aaaacctgct gcaaaagcca gtgttttacc acttgtggtt tttcttcgcg attgcggtga
     4441 tttatctggt ttcaccgctg attcaggtga agaacgtcgg cggaaaaatg ttgctggtgc
     4501 taatggtggt gattggcatt atcgctaacc caaacacagt gccgcagaaa attgacggtt
     4561 ttgaatggct gccaattaac ttatatatca atggcgatac tttttactac attctgtatg
     4621 gcatgttggg ccgcgctata gggatgatgg acacacagca taaagcactg tcgtgggtga
     4681 gcgccgcgct gtttgcgacg ggggttttta ttatctctcg cgggacatta tatgaattgc
     4741 agtggcgcgg aaattttgcc gatacctggt atctttactg tgggccgatg gtttttatct
     4801 gcgcaatcgc gctattgact ctggttaaaa acacgctgga tacgcgtacc attcgcggac
     4861 ttggcttaat ctcccgccat tcattgggta tatacggatt ccacgccttg attatccatg
     4921 cgctgcgcac ccggggaatt gagcttaaaa attggccaat actggatatt atttggatct
     4981 tttgcgcgac gttggcagcg agtttgttac tttctatgct ggtacaacga atcgacagaa
     5041 acagattagt gagttaagta aaagcccgtt cacattggac tgaccgggct tacgtgaatt
     5101 attcgctgta ttcttcctgg gtaacctgtg tttcttttgg cgggtttatt ccggtatcac
     5161 gaatattctt ctgcaccgct actgcaccaa acaagcttaa gaagaaagcc agtccataaa
     5221 aacctttttc actgagtaat aatgtcgcat tccacagacc taccatcagt aatgcaacag
     5281 agataataaa gacagtcagg caggtcatat aataaatgga agtggtcggt atgccttcat
     5341 acttgtcccg aacggtcttt tgataagacg cagcagaaaa cagtcctaac accagtacgg
     5401 caaaataata ccctttttca tttaactgca tctctgcatt ccatagccct aacagatagg
     5461 taacgatacc accaacgaga gctatccatg acacaatact aaaggccggt gaataggttg
     5521 atattttgtt gtccatcaca gtattccttt cattcctgaa tgtgtaagag ctttatgttg
     5581 ctcataccga gcgaaacaaa cgcatttgac caaacaagca gacaaaaaag gcgatggggt
     5641 agatcaccat ttcataagca ttgataggtg catttaaaac accgataaac aagagaccga
     5701 tagtgattaa cgcaactaac tggcagatat gggtaaaaaa attatccagt tgcccgagtt
     5761 tttctgcgcg aagatatgca tagttaccaa aagttgctgt cattaacacg ccaagaaaat
     5821 agccttttcc acttagcaat gggcatgaga tccatagccc aaccagataa accagcgccc
     5881 cgacaacaaa taatagtttt gccacagttt ttgaggtttt cataacgatc tccatatcta
     5941 ccagcgatac attacgagta accaacgaaa gacaaaactg aaaaatgcca ttaacaaatg
     6001 attttcagaa ta
//
LOCUS       DAZGJW010000225         5928 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZGJW010000225 DAZGJW010000000
VERSION     DAZGJW010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.227"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            268..978
                     /locus_tag="ACHKIK_004722"
     CDS             268..978
                     /locus_tag="ACHKIK_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO9085736.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            1020..3671
                     /locus_tag="ACHKIK_004723"
     CDS             1020..3671
                     /locus_tag="ACHKIK_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO9085737.1"
                     /translation="MTAFRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKIALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVITVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            3685..4215
                     /locus_tag="ACHKIK_004724"
     CDS             3685..4215
                     /locus_tag="ACHKIK_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9085738.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4228..4731
                     /locus_tag="ACHKIK_004725"
     CDS             4228..4731
                     /locus_tag="ACHKIK_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9085739.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            4790..5704
                     /locus_tag="ACHKIK_004726"
     CDS             4790..5704
                     /locus_tag="ACHKIK_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9085740.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         1737 a         1259 c         1300 g         1632 t
ORIGIN      
        1 catttttaat gagaaatggt ttgtatttgg agcgctatga aaaatcacat aatatctaaa
       61 ctatcaataa acaacagtag ttaaacttac tgttatttaa aatatttact ttgtagcaag
      121 gttttcctat taaaagagaa ccaacattta ttacaaacgc attaagtctg gaaacccggc
      181 gactcgccaa tgtattagca acattaatcg attgtctgat gtaacggttt tcccgcttat
      241 cctcttaaca catggagaat gcatcagatg caaactacgc gtacccccta ctctatttcc
      301 tttatggcga cagtgttact tttattgctc tttgcttgcc atagcaccgt tgccaacgca
      361 gctgtcgcac tgggtgcgac gcgggtaatt tatccggcaa accagaaaca ggttttattg
      421 cctgtcacga ataatgatcc tgccagtgtc tatctcattc agtcatggat agaaaatgca
      481 ggtgaccaaa aagacaccca gtttgtcatt accccaccgc ttttttccat gcaaggtaaa
      541 aaagagaata cgctgcggat aatcaacgct actaatcatc agttaccagg cgaccgtgaa
      601 agcctgttct gggtgaatgt taaagctatt cctgctatgg aaaaagacca gaagaatgag
      661 aatacattac agctggcaat tattagccgc ataaaaatgt tttatcgccc cacgaacctg
      721 gcaatggcac ctgaagaagc gcctgcaatg cttcggtttc gtcgcagcgg tagcaaactc
      781 acgcttatta atccaacgcc ctatttcatt accgtaacaa atatgaaagc aggaaatagc
      841 aatctcccaa acaccatggt gccgccaaaa ggtgaagtat cggtggatat cacgcatgct
      901 gcaaccgggg atatcagttt tcagacgatc aatgattacg gtgcattaac tccgcgtatt
      961 aaggcaacca tgcagtaagc cagtgaccta attaataata aactcaatcg gaattaataa
     1021 tgacagcctt tcgtgccgca tttaaggcat atcgtatgca tcaggtgtta ataatgcctc
     1081 gctttgcacg gcttaccatt gcccttggct tagcaactgc ggtatttccc gttgacgcag
     1141 agtattattt taatccacgg tttttaagca atgatcttgc tgaatctgtc gatttatccg
     1201 cctttactaa gggacgcgag gcaccacctg gtacatatcg ggtcgacatt tatttgaacg
     1261 acgaatttat gactagtcgg gatattactt ttattgcaga tgataataac gcagaactca
     1321 tcccatgcct gagtacagat cttcttgtca gtcttggtat aaaaaaaata gcgttattag
     1381 acaataaaga acattcggca gaaaaacatg tgccggacaa cagcgcatgc acaccacttc
     1441 aggatcgttt ggccgacgca tcaactgagt ttgatgttgg tcagcaacat ttgtcactct
     1501 ccgttccgca aatttatgtt ggcagaatgg ctcgcggcta tgtttctcct gatctgtggg
     1561 aagaaggaat aaatgctggg cttctaaact atagttttaa tggtaattct attaataatc
     1621 gtagtaacca taatgcagga aaatccaact atgcatattt gaatttacag agtggcatca
     1681 acattggtag ttggcgactg cgcgataact caacgtggag ttataacagt gggagtagca
     1741 attcatctga cagcaataaa tggcagcata tcaatacgtc ggctgaacgt gacattattc
     1801 ccttacgctc acggttaacg gtaggtgata gttataccga tggcgatatt tttgatagtg
     1861 tgaactttcg tggcctcaaa ataaattcaa cagaagcgat gttgcccgat agccaacatg
     1921 gtttcgctcc ggtgattcat ggtattgccc gtggaaccgc acaagtgagt gtaaaacaga
     1981 acggatacga tgtttatcag actactgttc cacccggccc ttttactatt gatgatatca
     2041 actctgcggc caatggtggt gatctgcaag taaccataaa agaggcagac ggcagtattc
     2101 agacattata tgttccttat tcgtctgttc cggttctcca acgtgctgga tatacgcgtt
     2161 atgcgcttgc catgggggaa tatcgtagtg gaaataacct gcaaagctct cccaagttca
     2221 tacaaggtag cttgatgcat ggtctggaag gaaactggac accttatggc ggaatgcaaa
     2281 ttgcagaaga ttatcaggcc ttcaaccttg gtattgggaa agatttagga ctttttggtg
     2341 ccttttcttt cgatatcacg caggccaata cgacacttgc agatggcacc cgtcacagtg
     2401 ggcaatcggt taaatccgtc tacagcaaat ccttctacca gacaggaacc aatatccagg
     2461 tcgcaggata tcgctattct acgcaaggtt tttataactt atccgacagt gcctacagtc
     2521 gaatgagtgg ttacaccgtc aagcctccta ccggagacac caatgagcag acacaattta
     2581 ttgattattt taatctgttc tacagtaagc gtggtcagga acaaataagc atctctcagc
     2641 agcttggaaa ttacggtacg acatttttca gtgccagtcg ccaaagttac tggaacacgt
     2701 cacgcagcga ccagcaaata tcatttggat taaatgtgcc gtttggtgat attacgactt
     2761 cgctgaatta cagctattcc aataatatat ggcaaaacga tcgggatcat ttactcgctt
     2821 ttacgcttaa tgttcccttc agtcattgga tgcgtacaga cagtcagtcg gcatttcgta
     2881 attcaaacgc cagttacagt atgtcaaacg atttgaaagg cggcatgacc aatctatcgg
     2941 gggtttatgg cactctgctg ccggataata acctgaatta tagcgttcag gtcggtaaca
     3001 cccacggagg taatacatcg tctggcacca gtggttacag ttctcttaat tatcgtggag
     3061 cttatggtaa tactaatgtc ggttacagtc ggagtggtga cagcagccag atttattacg
     3121 gaatgagtgg tgggattatt gctcatgctg atggcatcac ctttggacag ccgctgggcg
     3181 acacaatggt tctggttaag gctcctggtg ctgataatgt caaaatagag aaccagaccg
     3241 gaattcatac cgactggcgt ggctatgcca tattaccatt tgcgacagaa tatagagaaa
     3301 accgtgttgc tcttaacgcg aattcccttg cagataatgt tgaactggat gaaaccgtga
     3361 tcactgtcat cccaactcac ggtgctattg ccagagcaac atttaatgca caaatcggcg
     3421 ggaaagtatt aatgacgttg aagtacggta ataagagtgt tccattcggt gcaattgtca
     3481 cacacggaga gaataaaaat ggcagcattg tcgcagaaaa cggtcaggtt tatctgactg
     3541 gacttccaca gtcagggaaa ttacaggttt catggggcaa agataaaaac tcaaactgta
     3601 ttgtcgagta caagcttcct gaagtttctc ctggtacctt actgaaccag cagacagcaa
     3661 tctgtcgcta aggaacaata gattatgaaa tacaataaca ttattttcct cggtttatgt
     3721 ctggggttaa ccacctattc tgctttatcc gcagatagcg ttattaaaat tagcgggcgc
     3781 gtcctcgatt atggctgcac agtctcatcg gattcgctta attttaccgt agatctccaa
     3841 aaaaacagtg ccagacaatt tccaacgacc ggtagcacaa gtccagccgt cccttttcag
     3901 attacgttaa gtgaatgcag caaagggaca acgggggttc gggttgcatt taacggtatt
     3961 gaggatgcag aaaataatac tttgttgaaa ctggatgaag gaagcaatac ggcctccggt
     4021 ttgggtatag aaatattgga cgcaaatatg cgtccggtga aactgaatga ccttcatgcc
     4081 gggatgcagt ggatcccact ggtaccagaa cagaacaata ttttgcctta ctccgctcgt
     4141 ctgaagtcaa ctcagaagtc cgtcaatccg ggactggtga gggcttcggc aacctttacc
     4201 cttgaatttc aataatcaga gtcagatatg aaaagacttc acaagaggtt cctgttagct
     4261 acgttttgcg cgttattcac agcaactctc caggccgccg atgtcactat cactgttaat
     4321 ggtcgggtag tcgctaaacc ctgcactatt caaaccaaag aagctaacgt taatctcggg
     4381 gatctttata cgcgcaatct gcaacaacct ggttctgcat ctggctggca caatattact
     4441 ttgtcattaa ccgattgtcc ggttgaaaca agtgcagtga cggcaatcgt gacaggttca
     4501 actgacaata cgggttatta caaaaatgaa ggtactgccg aaaatattca gatagagctg
     4561 agggatgacc aggatgctgc gttaaaaaat ggcgatagca aaacggttat tgttgatgag
     4621 atcactcgta atgcacagtt tccacttaag gcaagagcta tcacggtgaa tggaaacgca
     4681 agccagggaa cgatcgaggc gctaatcaat gtgatctaca cctggcaata acgtcgattc
     4741 gattttctta gccttctttt taaatcaatt tttaacctta ggatatatca tgggtaaaac
     4801 aatcagtata aaggtcctgt tcggtattta tcttctatta atggcaggca aagtttttgc
     4861 cttcagttgt aatgtcgatg gcgggtcaag tattggagca ggtacaacct ctgtttatgt
     4921 taatctcgac cctgtaatac agccgggcca gaatctggtt gtagacttgt ctcagcatat
     4981 aagttgctgg aatgattacg gcggctggta cgacactgat catataaacc tggtacaagg
     5041 ctcagctttc gcaggctccc tacagtcata taaaggatcg ctgtactgga ataatgtgac
     5101 ctaccccttc ccgttgacga caaataccaa cgtattggat attggtgata agacaccgat
     5161 gccattaccg ttaaaactct atattacccc agttggggcg gccggtggag ttgtcataaa
     5221 agcgggggaa gtcattgccc gtatacatat gtataaaatt gcaacattag gaagcggaaa
     5281 tccgcgtaat tttacgtgga atattatttc aaataacaat gttgttatgc caacaggcgg
     5341 ctgcaccgtt gatagtcgta atgtcaccgt ggatctcccg gactttcctg gaagcgcaga
     5401 aatacccctt ggcgtatatt gctcaagcga acaaaagctc tcattttatc tttcgggtgc
     5461 caccactgac agtagccgac aagtgtttgc taacacagca cctgatgcaa caaaagcatc
     5521 gggagtgggt gtcactctaa tgcgtaatgg aaaaatactg gctacgggag aaaatgtctc
     5581 cctggggaca gtaaataaaa gcaaagtacc tctgggttta agcgcaacct atggacaaac
     5641 aggaaacaag gtttcagccg gaaccgtcca gtcagtcatt ggtgtcactt ttatctatga
     5701 ataattgtga ttttgacctt agtctatgcg atccattcac gaaaattgcg cggataatta
     5761 ttttgtgaag gctattagcc tacacctgta agaaaatccg cgcacaattg aagccagagt
     5821 cttatttttt atattgaaaa atatgttaat ttaaacacta tgagagatga tgcttaactt
     5881 atcgtattta atctattgtt taacgataat agattaaaaa tagaaaga
//
LOCUS       DAZGJW010000226         5897 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZGJW010000226 DAZGJW010000000
VERSION     DAZGJW010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.228"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(57..443)
                     /gene="ridA"
                     /locus_tag="ACHKIK_004727"
     CDS             complement(57..443)
                     /gene="ridA"
                     /locus_tag="ACHKIK_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO9085741.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(516..977)
                     /gene="pyrI"
                     /locus_tag="ACHKIK_004728"
     CDS             complement(516..977)
                     /gene="pyrI"
                     /locus_tag="ACHKIK_004728"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO9085742.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(990..1925)
                     /gene="pyrB"
                     /locus_tag="ACHKIK_004729"
     CDS             complement(990..1925)
                     /gene="pyrB"
                     /locus_tag="ACHKIK_004729"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO9085743.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(1929..2063)
                     /gene="pyrL"
                     /locus_tag="ACHKIK_004730"
     CDS             complement(1929..2063)
                     /gene="pyrL"
                     /locus_tag="ACHKIK_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO9085744.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(2344..2739)
                     /locus_tag="ACHKIK_004731"
     CDS             complement(2344..2739)
                     /locus_tag="ACHKIK_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO9085745.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(2870..3583)
                     /gene="bdcA"
                     /locus_tag="ACHKIK_004732"
     CDS             complement(2870..3583)
                     /gene="bdcA"
                     /locus_tag="ACHKIK_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9085746.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3654..4247
                     /locus_tag="ACHKIK_004733"
     CDS             3654..4247
                     /locus_tag="ACHKIK_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO9085747.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKVGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            4392..4844
                     /gene="tabA"
                     /locus_tag="ACHKIK_004734"
     CDS             4392..4844
                     /gene="tabA"
                     /locus_tag="ACHKIK_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO9085748.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            4967..>5897
                     /locus_tag="ACHKIK_004735"
     CDS             4967..>5897
                     /locus_tag="ACHKIK_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085749.1"
                     /translation="MAKISDLNCYQHMASADNVKPKNEVLNTWCVGMNDFARNAEGQD
                     SRRNILSPGAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMNF
                     VMNNPEGERADSSKVIIEISLSTTTTMGTRQGHTAIIFPQSDFLSFRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKNMALLLNNHQLNWKDAIDYNELLEKPLASTLEKIKKEH
                     LLLMPHVCDDTIPYLLGEGGILEEINDLKALHDTRIASGTEGNDEINNIKINLSNILI
                     DSLDDAKVNLEKVIDSMLKTFLKLPYINDVKILE"
BASE COUNT         1550 a         1338 c         1434 g         1575 t
ORIGIN      
        1 ttttgcgttt gtcatcagat ttaatccggg catgatagcc cggatttcca tcaagattag
       61 cgacgaacag cgatcgcttc gatctcaatt tttacgtctt tcggcagacg ggcaacttca
      121 acgcaagaac gtgccgggaa ggtggcattg tgttcggtga agaaggcttc gtaggtagca
      181 ttaacggttg cgaagtcgtt cagatctttc acaaacacgg tggttttaac gatgtcgccc
      241 actttcaggc ctgcggcttc gacgatcgct tttacgttat ccagcgactg acgtgcctgt
      301 gcagcgacgt ctgccggtac ttcgcccgtt ttcggattta ccgggatctg gccggaggtg
      361 atgatcatat tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt
      421 tccgtcgcga tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc
      481 attataggga gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg
      541 gaaaactctt tttcacagta tttgcatttg agcgcgatgt cattggcgcg ttttcgcacg
      601 gcaaagctgg aagaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc
      661 acattgtcga tgcgctccgg cagactgggg cgcgatttac ccaccacttc atagttgtcg
      721 atacggttaa ccgtggcttg cggcgcatac aatgccagtt gatctacctg atcttcactc
      781 aaaaaggtat tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaggttc
      841 agaccaatgg tgatgcgctg atccgtttcg gtcagcttga acagactcaa cagcttaaaa
      901 ccgatctggg cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat
      961 ttattatcgt gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt
     1021 gccagtaacg cctggcgagc gaaaatcccg ttgcctgcct gctggaagta ccaggcgtgt
     1081 ggcgttttat caacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc
     1141 atattggctt tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg
     1201 gcgtactcgg acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact
     1261 tccgccatca cttcttcaat agagctgtgc agactccatg cgatcccttt ttcatcgagc
     1321 atatccagaa tgtattgcgg cattgccagc gcgtccggcg cgatgaagta aaaacggttg
     1381 ccatcgaact tcgctaacgc ctgagtcagg gagtgaacgg tgcggccata tttcaggtca
     1441 ccaaccattg cgacgtggag attgtccaga cgcccctggg tttcctgaat agtgaataag
     1501 tccagcaagg tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca
     1561 ttgccggaaa actcggtggc caggcgcgcc gcaccttcct gcggatgacg catcactatc
     1621 gcatcgacgt aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ctttttaccc
     1681 agtgatgtat tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatagat
     1741 gtttcgaaag agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg
     1801 tgcttcaaca gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga
     1861 ttaaggtcat cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta
     1921 gccatctttt atctcctgac gcctgggcaa aaaaaagccc ctcgattgag gggctgggaa
     1981 tgggtgatca acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca
     2041 aaatgtcgaa cacactgaac catacatcct cccggcaaat tgtccggcat tatactcata
     2101 gccagaagcg gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat
     2161 taaaagtcaa aatgtatgca tttttatttg ctttgattct ggaggagaac tgtcgttcgg
     2221 tatcaataat tgcagacatt tcctgctttt ttctttttca ctttcacgca atcagatatg
     2281 cattttattc cattcatccg acttataggg cggagtgtga aaacgaacgg ctaacactat
     2341 tgcttacagc tcagggatgc gcgctatcac tttaatttca aaatcaaagc ctgccagcca
     2401 ggtaacgccc accgccgtcc agtttggata aggaggggcg ctaaatattt catttttgac
     2461 cgtcatgatg tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat
     2521 atcgtcaaaa gtgcatcccg cagcagccag ggtcgcatgc aaattatcaa atgccagtct
     2581 gacttgttgc tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga
     2641 aacaaacagc aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga
     2701 atgtcggcca gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac
     2761 gacatgaaca gaagatttct ctttaccggg agccgctttt agcggacgac gtgagtaaac
     2821 aaaacccaga catcatggat aatggctggg cttaattgag cgtagtcggt tatgcgccaa
     2881 acgcgccatc aatggtatgc atcgcgccgg taacaaaact ggcttctggc cctgctaacc
     2941 atgcgaccat accagcgacc tcttccggtt gcccatgtct tttgatagcc atcaaactat
     3001 gcaacatatc gcgcattggc ccgttggcgg gattagcgtc ggtatcaatt ggccctggct
     3061 ggacgacgtt aatggtgatc ccacgcggtc caaaatcacg ggccagcccg cgcgccatgc
     3121 cttgcagggc agatttgctg gcggcataag cagccatgcc tgcaacaggc atacgatcgc
     3181 cattcacgga gccgatgatt aagatgcgcc cgccttcggg catctgccgg gcggcttcaa
     3241 cagaggcatg ataaggagca tgaatattga ttttgaaaag gcgatcaata tcgtcggcat
     3301 ttaattccag ggcatcgcca aagacgccaa tacctgcatt taccaccagg atatccaatg
     3361 cgccgctctt acgaacgaca tcaatgacag cgtctctgtc agcactatct gtaaatactg
     3421 ctgtcgctcc agtctcttgt gccagatgtt cagcggcatc tttcgacccc gcataggtga
     3481 atcgtacatt ggccccatca gtgacgaaac gacgtacgat agcggcaccg attccacgac
     3541 tgccaccgag gatgagaact gtcttacctg taaaagcgcc cataaggact ccttgattta
     3601 ttatgtaaca tgcattacaa aactgtttta actttctgtc aacaagtttt gtaatggtca
     3661 ctaaaaaaca atctcgcgtt ccaggtcgtc ccagacgttt cgctcctgag caggcagtct
     3721 ctgcggcaaa agtgcttttt caccaaaaag gtttcgatgc tgtcagtgtt gctgaagtta
     3781 ctgattatct tggtattaac cccccgagcc tctacgcagc ttttggcagt aaagttgggt
     3841 tatttagccg tgtactcaat gaatatgtcg gtacggaagc tattccgctt gccgatattc
     3901 ttcgtgatga tcgtccggta ggcgagtgcc tggttgaggt attaaaagaa gcggcgcgca
     3961 gatatagcca aaacggcggc tgcgctggct gtatggttct tgaaggtatt catagtcatg
     4021 atccacaagc gcgtgatatt gccgttcaat attatcacgc cgcagaaacg accatttatg
     4081 actatatcgc caggcggcat ccacaaagcg cacaatgtgt gactgatttt atgagtaccg
     4141 tgatgtcagg gctttctgcg aaggcacggg aggggcactc catcgaacaa ctctgtgcaa
     4201 cagctgcact ggcgggggaa gcgataaaaa ctattctcaa ggagtgatgc tggccttgac
     4261 ccgaaaggcg ggaacgcgcc tgccgataag ttgtgataag acaataattc acgcgttaag
     4321 gctagcggaa ttgatcatct tttcgtataa cggtggaaat gaaacattgt tttaattaag
     4381 gagtggaaaa gatgatcatc ggaaatattc ataatcttca gccgtggcta ccccaggagt
     4441 tacgccaggc gattgagcat atcaaagcgc acgttacggc agaaacgcca aagggtaagc
     4501 acgatatcga aggcaatcgt ctgttttatc ttatctcgga agatatgacc gagccgtacg
     4561 aagctcgccg tgcggagtat catgcccgct atctcgacat tcagattgtg ttaaaaggtc
     4621 aggaaggcat gaccttcagc acgcaacctg caggcacgcc ggataccgac tggttagctg
     4681 ataaagacat cgcatttttg ccggaaggcg ttgatgagaa aacagttatc cttaatgaag
     4741 gtgattttgt tgtgttttat ccgggggaag tgcataaacc gctgtgcgca gtgggcgcgc
     4801 cagcccaggt tcgcaaagca gtagtgaaga tgctgatggc gtgatgactt ttcgccgtaa
     4861 ataactccag gtttacggcg agtttgtgaa aagagcgttt tttgatattt ttttgtgagt
     4921 agaatttgta atgcttagac gttcttatta actcaaggag tccgtcatgg caaaaatatc
     4981 agatttgaat tgttatcaac acatggcatc agccgacaat gttaaaccaa aaaatgaagt
     5041 cttaaatacc tggtgtgtgg gaatgaatga ttttgctcgt aatgccgagg ggcaggatag
     5101 cagaaggaat attctatctc ctggagcatt tttggagttt ttggcaaaaa tatttaccct
     5161 gggttatgtg gattttagca aacgctccaa cgaagcgggt agaaatatga tggctcatat
     5221 tgagtcctca tcttatatca aaaataatga tggcagtgaa ataatgaatt ttgttatgaa
     5281 taatcctgaa ggtgagcgtg cggattcatc taaggtgatt atagaaattt cactttctac
     5341 taccactact atggggactc gtcaaggaca tacagccatt atatttccac aatctgattt
     5401 tttgagtttt cgttatgaag gtaagtcttt tgaaagaaaa gatgatagtt cattacatct
     5461 gattactaac aaggttctgg cgtgttacca acgcgaagct aacaagaata tggcgcttct
     5521 attaaataat catcagttga actggaagga cgcaattgat tataacgagt tattagaaaa
     5581 gccattagcc agtacattag aaaaaataaa gaaagaacat ctgctattaa tgcctcatgt
     5641 atgtgatgac acgattccat acttactagg cgaaggtggt atacttgaag aaataaatga
     5701 tctgaaagcg ttacatgata ccagaattgc tagtggtaca gaaggtaatg atgaaattaa
     5761 taacatcaaa attaacctgt ctaatattct tattgattcc ttagatgatg caaaagttaa
     5821 tctcgaaaag gtcatcgatt cgatgttgaa gactttttta aaattgccat atattaatga
     5881 tgtaaaaatt ctggagt
//
LOCUS       DAZGJW010000227         5875 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZGJW010000227 DAZGJW010000000
VERSION     DAZGJW010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.229"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            49..288
                     /locus_tag="ACHKIK_004736"
                     /pseudo
     CDS             49..288
                     /locus_tag="ACHKIK_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(336..953)
                     /gene="ureG"
                     /locus_tag="ACHKIK_004737"
     CDS             complement(336..953)
                     /gene="ureG"
                     /locus_tag="ACHKIK_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFO9085750.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLDLIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(965..1639)
                     /locus_tag="ACHKIK_004738"
     CDS             complement(965..1639)
                     /locus_tag="ACHKIK_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFO9085751.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(1640..2104)
                     /gene="ureE"
                     /locus_tag="ACHKIK_004739"
     CDS             complement(1640..2104)
                     /gene="ureE"
                     /locus_tag="ACHKIK_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFO9085752.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(2114..3784)
                     /gene="ureC"
                     /locus_tag="ACHKIK_004740"
     CDS             complement(2114..3784)
                     /gene="ureC"
                     /locus_tag="ACHKIK_004740"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFO9085753.1"
                     /translation="MFGPTTGDKIRLADTELWIEVEDDLTTYGEEVKFGGGKVIRDGM
                     GQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNPDIQPNVTIPIG
                     VSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTGPTAGSNATTCT
                     PGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKIHEDWGATPAVI
                     NCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGAGGGHAPDIITA
                     CAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAESRIRQETIAAE
                     DVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEESGDNDNVRVKRY
                     IAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKGGMIAMAPMGDI
                     NGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLHSTTAVVKGCRT
                     VQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFLF"
     gene            complement(3810..4130)
                     /locus_tag="ACHKIK_004741"
     CDS             complement(3810..4130)
                     /locus_tag="ACHKIK_004741"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFO9085754.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVDLVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(4139..4441)
                     /locus_tag="ACHKIK_004742"
     CDS             complement(4139..4441)
                     /locus_tag="ACHKIK_004742"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFO9085755.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(4451..5230)
                     /locus_tag="ACHKIK_004743"
     CDS             complement(4451..5230)
                     /locus_tag="ACHKIK_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFO9085756.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGPLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQQVMRDIWQFMRPQLTDKSPVLPRIWLT"
     gene            5611..>5875
                     /locus_tag="ACHKIK_004744"
     CDS             5611..>5875
                     /locus_tag="ACHKIK_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085757.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDD"
BASE COUNT         1320 a         1536 c         1585 g         1434 t
ORIGIN      
        1 ctgtagattc aatctgtcaa tgcaacaccc ctttcaatta tctctttcac tgcccgcgac
       61 ggtatcggtt atcagtttga cctgctggct aacggtgaaa ccgtactgcg tgatgcatcc
      121 ggcgaaaaac gcatcaaggg tgaaaaagac ggtcgtatgc tcatgaatgt tggcctgaat
      181 gcggaaattc gcgacaacat ccgcttcggt ctggagtttg aaaaatcggc atctggtaag
      241 tacaacgtgg ataacgcggt gaacgccaac ttccgttatt ccttctgata aaaacaaggg
      301 gccgcctgcc ggccctgata cagaggatgt tgtgattact tacccagcat gccctgctct
      361 tcgataaagg cgatgatgtt ttgcagacct tcaccacttt tcaggttagt gaacccccat
      421 gggcgttctc cgcgcatacg taaagtatca cgctccatta cctcgaggga ggcgcccacg
      481 tatggtgcaa ggtcagtttt attgatcacg aggaagtcgg atttggtgat ccccggaccg
      541 cctttacgcg ggatcttttc tccttctgcc acatctatga catatatggt aaggtccgcc
      601 agttcggggc tgaaggtcgc actcaggttg tcgccgccgc tttccacaaa gatcagatcg
      661 agattaccaa atttctcact cagtgcttcc acggctgcca gattcattga tgcgtcttca
      721 cgaatggctg tatgagggca gccccccgtt tcgacaccga caatgcgttc cgcttccagt
      781 gctccggcct cagtgagaat gcgttgatct tctctggtgt agatgtcgtt ggtgactacg
      841 gctaattgcc aggtgtcacg catggatttg cagagggctt ccagaagcgc ggttttacct
      901 gagccgaccg gaccgccgac gccgacacgc agcggttgct tgattatgtt cattttattt
      961 tctcctaaga acggaataat cgggaatact gggtttcatg tcgcgcggaa gcgatagatg
     1021 caagtggcgt tgctgaacca attgcgtgat ccggggttgc aagggcactg tccatgttcg
     1081 ccgcatagcg ggcacacagg cgcataatta actgctgggc agccagctgg ccgtagggta
     1141 ccagcctgac gcccgccatc actgcgcttt caatccagct gtagccaagg ctcagggcca
     1201 ggtctgaaac ggatatttgc cagcgtacgc cgagccatgc cataccggtt aactggcttt
     1261 gctggcatag tttgcgccat tcggccgggc agtcgggctg ccagtcgacc agaagtctgg
     1321 tgaaggctgc accgcggtta cgttcttcat cgcgtagctc acgggtttcc ctgcaggcca
     1381 gcagataagc ggtccagcgc ccggcactgt caggatcgcc cgcttcacag gcccggtata
     1441 gccgggcaag cagtggcagg tcgacggcaa aaaaactctg ttccatctgc aactgctgcc
     1501 agtgttcaaa ggcggcgcta tcttctaccc agcctgcttc cactgcccac tccagtccct
     1561 gggaccagct gtagcctcca acagggaggc tgttactggc aagctgcatc agccgcagac
     1621 gtttttccgg agtcggcatt cagtggctgt gctcctgatc gtgattatgg gcgtgcgact
     1681 tgctggcgta agcgccagct tccggctcaa acggaagatg tgcaaaatca acatccagcc
     1741 caaactggcg cagcatatca tcgaggacgt gatcgtgatg gtagcgcagc tctccgggca
     1801 ttatttgcag cggaacgtgg cgattgccga gatgccagca ggctttcgcg agcataaaag
     1861 gatctgcgca gcgcactaca gaaacggctt catcagcggc gatcacctta atgaattcat
     1921 caccgttttc gttactgaga atatcaccat cgcgcagcag cagaccgcgt ggcagcaaca
     1981 gtccagcctg acgtccatcg ttaagggtga ctttaatacg gctttttacc cgcatgtcaa
     2041 cagggagggt aacgctggca gttgtctgag ccggggtctc cacccgccgg gtcaaataaa
     2101 gcatttgtgc tccttaaaac aggaaataac gttgcgccat tggcagaatg tccgccggtt
     2161 cactggttat cagttcgccg ttgatacgca cttcgtaggt ttgtgaatcc acggttatat
     2221 caggcaacag gctgttgtgg cgcatatcgg ctttttgtac tgtgcggcag ccttttacca
     2281 cagcagttgt gctgtgcagg ttaagctgtt cagcgacgcc atttgctgct gctgcctgcg
     2341 acaggaaagt cacacgacag cggtgacggg cactgcccaa tgcagcgaac attgggcgat
     2401 agtgcaccgg ctgcggtgtg gggatagagc cgttgatatc acccatcggc gccatggcta
     2461 tcattccgcc tttgacgata gtcgccggtt ttacgccaaa gaacgccggg gaccagagca
     2521 ccagatccgc cagttttccc acttcaatcg agccgacttc atgagcaata ccgtgggtta
     2581 atgccggatt aatggtgtat ttagcgatat agcgcttcac gcggacgttg tcgttatcac
     2641 cactttcttc cggtaacggg ccgcgctgaa ctttcatccg gtgtgccacc tgccaggttc
     2701 gtaacactac ttctccgacg cgtcccatgg cctgcgaatc ggacgaggtg agggagaacg
     2761 cgccaaggtc gtgcaggacg tcttccgcgg caatggtttc ctggcgaatg cgcgattcgg
     2821 caaaggctac gtcctcggcg atatccgggt caagatgatg gcaaaccatc agcatgtcca
     2881 gatgctcatc aatagtgttg acggtatagg gtagcgtcgg attggttgag gaaggcagaa
     2941 tattggggtg cgcgcaggcg gtgataatat ccggagcatg accaccacct gcaccttctg
     3001 tatggaaggt atggatagtg cgcccgccga tggcagtcag agtatcctca acgaatcctg
     3061 attcgttaag cgtatcactg tgtagcgcaa cctgaacgtc catttcgtcg gctacagtca
     3121 gtgcgcagtt gattaccgca ggtgtagctc cccagtcttc gtgaattttg aggccgataa
     3181 ccccggccgc gacctgctca cgaagcgcat ccggattgga gcaattgcct ttacccagca
     3241 acccgatatt gaccggcagg ctgtctgcag cctgcagcat ttgataaatg taccatggtc
     3301 ctggggtaca ggttgtggcg ttagaacccg ctgtcgggcc agtaccgcca ccgatcatgg
     3361 tggtaatgcc ggatgtcagc gcttcttcag cctgctgtgg gcagatccag tgaatatgcg
     3421 tatcgacgcc acctgcggta acgatcctgc cttctgcggc aataatttcc gtggatacgc
     3481 cgattgggat agtgacgttg ggttgtatat caggattacc ggctttgccg atagcaaata
     3541 tccttccatc tttgacgccg atatcggctt taacgatccc ccagtaatcg atgatcaggg
     3601 cattggtcag caccagatca gcgcagccgg cggagagcat ttgcccctgt cccataccgt
     3661 cgcggattac tttaccgccg ccgaatttga cctcttcgcc gtaggtagtt aaatcatctt
     3721 cgacctcgat ccacagctca gtgtctgcca gacgaatttt atcaccggtg gtagggccga
     3781 acatgtcagc ataggcctag cgtgaaatat tactcatcat ttgcctccag ctcacccatt
     3841 atatctccgc ggaaaccgtg cacgatacgg gcaccgctca gcgccaccag atcaacctca
     3901 cgcttttggc ctggttcgaa gcgcactgca gttccggcgg cgatattgag acgatagcct
     3961 cttgccttct ggcgatcgaa cttcagtgcc gggttaacct ccgcgaagtg ataatgggaa
     4021 ccgacctgaa ttggtcggtc tccgtgattt tcaacgataa tactgcaggt agcccggccg
     4081 atgttaagct ctatatagcc aggtttaacc ttatattctc cagggatcat ctgttacctc
     4141 agattatcgg attatggacg gtaaccagct tggagccgtc cggaaaggtg gcttcgacct
     4201 ggatatcggg gatcatttct ggtatgcctt ccatgacctg ctcgcgactc aggacatgcc
     4261 gtccttcttc catcagcgca gcgacgcttt tgccgtcgcg agcgccctcc attataaaag
     4321 cgctaatcag ggccacggat tcgggatagt taagtttcag gccgcgggcc agacgacgct
     4381 ctgccagcag tgcagcggta aacagtaata gtttgtcttt ttctcttgga gtcagttcca
     4441 tagcgttctc ttaagtcagc cagatccggg gaagtaccgg tgatttgtca gtcagttgcg
     4501 gccgcataaa ttgccagata tcacgcatta cctgctggca acgcagatta tcatcactta
     4561 aaaagcgaac tgtcagtaga ccatcggtga gcgtggctcc ggcgtagttt tccagtggtg
     4621 tgagtttttc gcgtatgtct tccagttgcg tttcattccc caggtagaac agcatcgttc
     4681 ccacccatgg tttccgggca acgcaggtaa gttctccatt ggccacgtgc agacgctcaa
     4741 tcaacagcgg agaaccgtcg acccatatct ccagacggtt agcgagtgtt ccgtggctga
     4801 aggtttctcc gatgaccgga cgtccaaggc acagaagatc ccaggccagc agtgtactgg
     4861 aggacgacag atgaaacacg gtatgcaacg tggcgtgagc gccgggaaaa aaaatcgaat
     4921 cctgtggcaa ccactccagc gttgcgttat tgcacagcgt caggttttgc tgaaggtgcg
     4981 cctgcgcgcc gctgctgcgg taaaatttac tggcaccagg catggtgatc agcgtatggc
     5041 aatcagagtc aattattgcg ttgatgatga gttcgtcacc gccgacgata ccaccgggtg
     5101 gatgaagcag acagaggtgg caggtttctt cttcgggata aaaaggacgc tgaacagtga
     5161 gcggaccgac atgatgacgg gtggtgagcg tggttttccc gtgcagacgc tgaaaacgga
     5221 gatccagcat ggcctgccag ccttttttat tttgtggtaa cgcggtactg agcaatctta
     5281 cgcccctgat aaataaagat aaaacgacag taaagagccg gatttccttg ttatataaaa
     5341 gatgatgcct ttccagtgag ttttcgtctg aataatacga atgtttgttt tcgtcatgaa
     5401 gcgttccggc tcccggggct atacatcgcc agaaaagcaa tgcatattgt cgggattatc
     5461 cagagaatca ggcagaaaga tccgacaatg ggctgtgatt atgccatctg gctcagaatg
     5521 atatcaaggt gttatgggga taacttgctg atacaatagc agtatgacag attcattaat
     5581 gcggaaactg ctcactgaaa cagagtgttc atgactgagc actggtcggg tcatgcagat
     5641 gataatttcc ccttttcttg gggaagcgac agtattttca tgcatgcctg tcttcagtca
     5701 tacggggaga gcatctgtga tacagtccat acatttgttg cggacgtcgc tcctgagtca
     5761 ctgactggtc tgcaaacagt ttatctgtac acggcagggc ttctgtgtga tatcgttttt
     5821 catatcgagg gggggcctcc agagatgaat ggaataataa tatttcctga tgata
//
LOCUS       DAZGJW010000228         5753 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZGJW010000228 DAZGJW010000000
VERSION     DAZGJW010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.230"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1137
                     /locus_tag="ACHKIK_004745"
     CDS             <1..1137
                     /locus_tag="ACHKIK_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO9085758.1"
                     /translation="EYAKLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESEL
                     ETHLNKPPVPPQIRRTIFNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGI
                     INTLWDGGSLFIDRKSSPGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHAR
                     FLMCQPTSTQGTRIITGDNYSSQYQDLFEERINELIDESLAMSGERRCLHFSPQAARI
                     WTDYYNDVESKLGGLGPLRHCREYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMAR
                     ELAIWYGNEYVRLSNPLTFDNSALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDI
                     LQRGPNRFRKKDKINWLLDLLYEQNRVVPVIEGKTLCVAPNFDL"
     gene            1958..2521
                     /locus_tag="ACHKIK_004746"
     CDS             1958..2521
                     /locus_tag="ACHKIK_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9085759.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
     gene            2676..5036
                     /locus_tag="ACHKIK_004747"
     CDS             2676..5036
                     /locus_tag="ACHKIK_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO9085760.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(5198..5374)
                     /locus_tag="ACHKIK_004748"
     CDS             complement(5198..5374)
                     /locus_tag="ACHKIK_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085761.1"
                     /translation="MTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMA
                     NYQLDEQLLEGCRP"
     gene            complement(5512..5753)
                     /locus_tag="ACHKIK_004749"
                     /pseudo
     CDS             complement(5512..5753)
                     /locus_tag="ACHKIK_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         1543 a         1119 c         1436 g         1655 t
ORIGIN      
        1 gaatacgcga aactggttga gaattacagt acagaaaagg aaatatggga gcaaaaacaa
       61 aaagcgctgg aatcaaaatt tcataaagag attcgtgccg gtaaagatta caaggcaaca
      121 gaatcagagc ttgaaacgca tctgaataaa cctcctgttc cgccgcagat acgtcgaaca
      181 atttttaatg agacaacgat agagggaatg ttaaaatatt actccgatag caatcgctct
      241 tttgctcttg tatccagtga agggggggta atttttgaca gccgggccat gagtaaactg
      301 ggaattatta acactttgtg ggatggaggt tctcttttca tcgacaggaa atcatctccc
      361 ggaattaatt tgaaggagcc aagactgacg atatcggtga tgattcagcc tgatgtttac
      421 cacaaaggtt tttgtacgcg aaaaaaagaa attgtgaaaa cgtcaggaca tcatgcaagg
      481 tttttgatgt gtcaaccaac atcaacgcag gggacaagga taataaccgg agataattat
      541 tcatcacagt atcaggattt gtttgaagag aggatcaatg agcttattga tgaaagtctg
      601 gcgatgagtg gtgaacgacg ttgccttcac ttttctcctc aggctgccag aatctggacg
      661 gattattaca atgatgtgga atcgaagctg gggggattgg ggcctttaag acattgccgg
      721 gaatatgctg ctaaaaatgc agagtatatg gcaagactgg caggacttat ttaccattcg
      781 agcggtgaag agggggagat ttccccttac actgcagaaa tggcgagaga attagcaata
      841 tggtacggta atgagtatgt gcggttgtct aatccgttaa cttttgacaa ctctgccctg
      901 accgtacctg tgcgactcat tccagaggag cttgaacttt tcaactggat aaaaagctat
      961 tgtattgaga aggggatcct ctgtatgaaa aaaaatgaca ttttacagcg tggtccgaac
     1021 cgtttccgga aaaaggataa aatcaactgg ttactggatt tactgtatga acaaaacaga
     1081 gttgtaccgg ttattgaggg aaaaacgttg tgtgtcgcac ctaactttga cctctgaacg
     1141 acttaatcgt tgagtacata tagtctcctc tgctacttgc ggaggagact aatgtcttta
     1201 ccatgtcagc gttgtattag aaaactctgg tacaggtcag tcagtaaata taactggtgt
     1261 aaccactatt tactattgga tagagtgtta ctcaggatat tgtcggcttt agtctgaggt
     1321 gaggtggggc cttcattgtt ttcgtgttca ataaataatt taggttaatt agtattatta
     1381 gtggaagtta tgtgatctgt tatgttgtct tgtacgcact taacactggt tacgtaccgg
     1441 aacactatta gagtatgtta tggctctgtt acagtttata ggtatgaaaa aatataatct
     1501 ttcctgtatg aaataatatt cttatggtgt gtggggtatg gtctgtatac catcattttt
     1561 atggtgaagt taaatatctg tagaggcctc aagctgggtc gctgttttta cttaatggta
     1621 cagaagtatt aatagcggtg ttgcctgtgg ctacccgata ctggcttttg tgaatgtcag
     1681 attacttcct gtaagtgatt gaatggtact tccttaaact caattctatg aggtgtgttt
     1741 ttaccattgt aattcgtact ggtcctcagt ttaagtgagt cttactgtac tctgaaatat
     1801 tacctctttg gagtaatgag gtaatacgaa acagttttat caggttgtct gtgctgctgg
     1861 ttacctcgtt tattaatgcc gcggtacggc gtttctgtac tctcagtatt tatgtaattc
     1921 cactttcctg tcagttttat aaagtaagga ctataaaatg tataatgcta atcccaatta
     1981 cgaaatggat ttcatgattc tgaaagacgt gaatgaacac atggaaggtc tgtttcaacg
     2041 tttttctaaa ctgttacctt tcagaattga ttttgcctac aggaaagaca cgcccagttt
     2101 tggtcattcc tgtaggcaca gtatgtgtat tgaaatgcac cgcttactta gtgaaaccca
     2161 aacgatgctg gcgggatatt actgggtaat ggaatatacg tcaaacaagg gacttcatat
     2221 tcattttatc ggctacctgg atgggcaacg tcacaacaaa tcttatcgga tatccaggca
     2281 actgggagat atctggaggc ggatcacgga aggggagggt tattttcacc tgtgcagggc
     2341 taaagacaaa tacccggtgc gtatcgatca cgtaattcac tatagtgata aatctgcggt
     2401 agacgactta cgttacgccc ttagctatct ggccaaacag gatcagaaag aacacggtat
     2461 tattctgagg cgtagcaggc ttcccgaaaa aagtaaccgg ggccgaccac gacataactg
     2521 aattacagct gtattaggta ttagaattaa gtttctgtaa gttgttggga ggtgcttgag
     2581 ttaataggca gaagagggcg gggcgctttg tcatcagtgt aggggacccg aatttctgcc
     2641 gtggtgcctc agtggatcgg atacaggtaa cgaatatggt tcataaatct gacagtgatg
     2701 aattagctgc attgagggca gaaaatgccc gtctggtctc attacttgaa gctcatggga
     2761 ttgaatggcg acgtaaaccg cagactcctg tgcagcgcgt ttctgtatta tccaccgacg
     2821 agaaggttgc attatttcgt cggttgtttc gtgggcgtga tgatgtatgg gcacttagat
     2881 gggaaagtaa aaccagcggc aaatcagggt actctcctgc ctgcgctaac gaatggcagg
     2941 cgagaatatg cggtaaaccc cggataaaat gtggggactg cgctcaccgt cagttgattc
     3001 ctgtatccga tctcgtcatc taccaccatc tggccggtac ccatactgtc gggatgtatc
     3061 cgttgctgga agatgattcc tgttattttc tggcagttga ttttgatgaa gctgaatggc
     3121 agaaggatgc atccgcattc atgcgatcct gtgatgagct gggtgtacct gctgcgctgg
     3181 aaatatcccg ttcacgtcag ggggcgcatg tctggatatt ttttgcctca cgagtttcgg
     3241 cccgcgaagc tcgccgtctg gggactgcta ttattagcta tacgtgtagt cggacccgac
     3301 agctgcgatt ggggtcttat gaccgattat ttcctaatca ggatactatg ccaaaagggg
     3361 gatttggtaa tctcatagcg ttacctctgc aaaaaagacc gcgtgaattg gggggaagcg
     3421 tttttgttga tatgaatctc cagccttatc ctgatcagtg ggcttttctt gtatcggtga
     3481 ccccgatgaa tgtgcaggat attgaaccga cgatattacg ggctacaggg agtatccatc
     3541 ctctggatgt gaattttatc aacgaagaag acctgggtac gccgtgggaa gagaaaaaat
     3601 catcaggaaa cagactgaat atttctattg cagaaccgct gaaaatcacg ctggcaaacc
     3661 agatctattt cgaaaaagcg caattacctc aggtgctgat taaccgactt attcggctgg
     3721 cagcatttcc gaaccctgag ttttataagg ctcaggcaat gcgtatgtca gtctggaata
     3781 agccccgtgt tataggttgt gcggagaatt acccgcaaca cattgcgttg ccccggggat
     3841 gtctggacag cgtattatct ttccttaggg acaacaatat tgctgcagaa ttaatcgata
     3901 aacgatttgc cgggacggaa tgtaatgccg tttttatggg aaacctcaga gcggagcagg
     3961 aagaggccgt ttcggcatta ctccgttatg acactggtgt gctttgtgcg ccaacggctt
     4021 ttggtaagac agttaccgca gcggcagtga ttgccaagcg gaaagtgaat acactgatac
     4081 tggtacaccg gactgaattg ctgaagcagt ggcaggagcg tctcgcggtg tttctgcagg
     4141 ccggtgacag tattggtatt atcgggggag gtaaacataa accctgtggc aatattgata
     4201 ttgcggtggt gcagtccata tccagacaag gagaagttga acctctggtc aggaattatg
     4261 ggcaaatcat tgtggatgag tgccatcata ttggcgcggt ttcattttct gcgattctga
     4321 aggaaacgaa tgccagatat ctgcttggcc tgacggcaac accaatccga cgggatggtc
     4381 tgcatcccat tatttttatg tactgtggtg ccattcgcca tacagcggtc cgcccgaagg
     4441 aaagcccaca taatctggag gtactgatcc gttcccgttt tacatctggt catttaccat
     4501 cggatgcgag aatccaggat attttcagag aaattgctct ggatcatgac agaacggtgg
     4561 cgatagctga agaagccatg aaagctttcg ggcaggggcg aaaagttctg gtactgactg
     4621 aacgtacaga tcatctggat gagatagcat cagtgatgaa ttcactgaaa ttgtctccct
     4681 ttattctcca tggtcgacta tcgaaaaaaa agcgtgcgat gctgatatcc gggctgaatg
     4741 ctcttcctcc cgattctcct cgaattttgt tgtcaacagg cagacttatt ggtgagggat
     4801 ttgaccaccc tccgctggat acgctgattc ttgccatgcc tgtgtcatgg aaagggacat
     4861 tacagcagta tgcagggcgt cttcacagag agcataccgg taaaagcgat gtcaggatca
     4921 ttgattttgt ggataccgcg tatcctgtgc tgctcagaat gtgggataaa cgtcagcggg
     4981 gttataaagc gatggggtac aggattatag ctgacggcga tgaatcagtg atttaatttt
     5041 aaggagtact ttaccctttt taaataattt gagctccccg ggttgttcgg gcaacaaaga
     5101 gtcccagtat acaggcttca ggaatatgtt gtagtatcag gcttttcatc gctttaaaat
     5161 gactaccggc agtgagaacg tcatcaaaaa taacaatcta gggcctgcac ccttccagta
     5221 attgttcatc cagttggtag tttgccataa tcgcttctgg taagggccgg tttgttgcgg
     5281 tatgagaggc tattgttgat acgttttgca caaccagttc ccagaaatcc agccatggtt
     5341 cgtaggtttg cgcaattcgc agaatttctg tcatacggtc atcgtaagcg gcatctgtac
     5401 ggcatttgga tggtggtacg ggaacaaatg tgtattcctc aagattaccg atatttgata
     5461 tgagcatctg agcaacatac tcaatagcag cctttttata gtggtaatca ggctacgaaa
     5521 gtacagcgtt ttataaattg tctcaggtga tattcgcagc gttttttgac gtggttttgt
     5581 tcgccttaac catcctgata tttgctctgg agaccatttc atctccagct tttccagaac
     5641 aagctttcgc aatggtaaat tttgatccag taagcacggt tttggccttt tcgccattct
     5701 gttggctcgg ttattagcat caacagcttt gtaatagcgt ctgccccgat tac
//
LOCUS       DAZGJW010000229         5676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZGJW010000229 DAZGJW010000000
VERSION     DAZGJW010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.231"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(146..622)
                     /locus_tag="ACHKIK_004750"
     CDS             complement(146..622)
                     /locus_tag="ACHKIK_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO9085762.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(681..1970)
                     /gene="bioA"
                     /locus_tag="ACHKIK_004751"
     CDS             complement(681..1970)
                     /gene="bioA"
                     /locus_tag="ACHKIK_004751"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO9085763.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2057..3097
                     /gene="bioB"
                     /locus_tag="ACHKIK_004752"
     CDS             2057..3097
                     /gene="bioB"
                     /locus_tag="ACHKIK_004752"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO9085764.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            3094..4248
                     /gene="bioF"
                     /locus_tag="ACHKIK_004753"
     CDS             3094..4248
                     /gene="bioF"
                     /locus_tag="ACHKIK_004753"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO9085765.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDIAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            4235..4990
                     /gene="bioC"
                     /locus_tag="ACHKIK_004754"
     CDS             4235..4990
                     /gene="bioC"
                     /locus_tag="ACHKIK_004754"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO9085766.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            4983..5660
                     /gene="bioD"
                     /locus_tag="ACHKIK_004755"
     CDS             4983..5660
                     /gene="bioD"
                     /locus_tag="ACHKIK_004755"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO9085767.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         1319 a         1520 c         1604 g         1233 t
ORIGIN      
        1 taacacattg atattaatgg tctttagcgt ttcgcgttca gcttttttat actaacttga
       61 gcgaaacggg aaggtaaaaa gacaaaaagt tgtttttaat acctttaagt gataccagat
      121 ggcattgcgc catctggcag agtgattaac taaacatcgc agtaatcgag gcgcttgcca
      181 gagagtggaa atgaacgtta aacccgacca tcgcgccgct ggcaccttca tcgacatcaa
      241 tacgttctat atccagcgcg tgaacggtaa aaatgtagcg atgagtttcg cctttcggcg
      301 gtgctgcgcc atcgtacccg gttttaccaa agtcggtacg cgtctgcaaa acgccgtctg
      361 gcattgctac cagaccagag ccaaaccctt gcggtaatac gcgggtatca gcgggtaagt
      421 taacaactac ccagtgccac cagccggagc cggttggcgc atccgggtcg tagcaggtga
      481 caacaaaact tttcgttccc gcaggaacat catcccacgc cagatgcggt gaaatattat
      541 cgccatcgta acccatgccg ttaaagacat gacgatgcgg caatttatcg ccatcgcgca
      601 gatcgttact gatgagtttc atgaaccctc ctttcttgtt tgcagaaagt gtagccagaa
      661 accctcacgc ggacttctcg ttattggcaa aaaaatgttt catcctgtac cgcgcggtta
      721 accgctgcgg tcagacgctg caactgttgc gggagaataa tatagggcgg catcaggtaa
      781 atcagtttgc caaaaggccg gatccagaca ccctgttcga caaagaattt ttgcagcgcc
      841 gccatattca ccggatgagt ggtttcgact acgccgattg cccccagcac gcgcacatcg
      901 gcaaccattt cggcatcacg ggcgggggca agttgctcgc gcagctgtac ttcaatagcc
      961 gccacctgtt gctgccagtc gccagattcg agaatcgcca ggctggcgtt tgctgccgcg
     1021 caggccagcg gattgcccat aaaagttggc ccatgcataa agcaaccggc ttcgccgtta
     1081 ctgatggttt ccgcaacctc gcgcgtggtg agcgtggcgg aaagggtcat tgtgccgccg
     1141 gttaaggctt taccgaggca caaaatgtcc ggcgcgattt ctgcatgttc acaggcaaac
     1201 agtttgccgg tacgaccaaa tccggtggcg atctcgtcgg caatcagcaa gataccttcg
     1261 cgatcgcaca ttttgcggat tcgttttaac cattccggat ggtacatgcg catcccgcct
     1321 gcgccctgga caatcggttc aatgatcacc gccgcgattt catgacgatg cgccgccatc
     1381 aggcgggcaa agccaaccat atcacgttca tcccattcac catccatgcg gctttgcggg
     1441 gcgggagcaa acaagttttc tggcagatag cctttccaca ggctgtgcat tgagttgtcc
     1501 ggatcgcaca ccgacatcgc gccaaaggta tcgccatgat aaccattgcg gaaggtcaga
     1561 aaacgctggc gcgcttcgcc tttggcctgc cagtactgca acgccatttt catcgccact
     1621 tccaccgcta cggagccgga gtccgcgaga aaaacgcatt ccagcggttg cggcgtcatt
     1681 gccaccagtt tgcggcatag ctcgatggcg ggcgcatggg tgataccgcc aaacatcaca
     1741 tgcgacatag catcaatttg cgacttcatc gccgcattaa gctgcgggtg attatagccg
     1801 tggatcgccg cccaccagga cgacataccg tcaaccaggc gtctgccgtc agacaaaatt
     1861 agctcgcaac cttcggcgct cgcgaccgga taaaccggca gaggggaggt catggatgtg
     1921 tatgggtgcc agatatggcg ttggtcaaag gcaagatcgt ccgttgtcat aatcgacttg
     1981 taaaccaaat tgaaaagatt taggtttaca attctacacc gaattaacaa caaaaaacac
     2041 gttttggaga agccccatgg ctcaccgccc acgctggaca ttgtcgcaag tcacagaatt
     2101 atttgaaaaa ccgttgctgg atctgctgtt tgaagcgcag caggtgcatc gacagcattt
     2161 cgatcctcgt caggtgcagg tcagcacttt gctgtcgatt aagaccggag cttgtccgga
     2221 agattgcaaa tactgcccgc aaagctcgcg ctacaaaacc gggctggaag ccgagcggtt
     2281 gatggaagtt gaacaggtgc tggagtcggc gcgcaaagcg aaagctgcgg gatcgacgcg
     2341 tttctgtatg ggcgcggcgt ggaagaatcc ccacgaacgc gatatgccgt acctggaaca
     2401 aatggtgcag ggggtaaaag cgatggggct ggaggcgtgt atgacgctgg gcacgttgag
     2461 tgaatctcag gcgcagcgcc ttgcgaatgc cgggctggat tactacaacc acaacctcga
     2521 cacctcgccg gagttttacg gcaatatcat caccacccgc acttatcagg aacgcctcga
     2581 tacgctggaa aaagtgcgcg atgccgggat caaagtctgt tctggcggca ttgtgggctt
     2641 aggcgaaacg gtaaaagatc gcgccggatt attgctgcaa ctggcaaacc tgccgacgcc
     2701 gccggaaagc gtaccaatca acatgctggt gaaggtgaaa ggcacgccgc tggcggataa
     2761 cgatgatgtc gatgcctttg attttattcg caccattgcg gtcgcgcgga tcatgatgcc
     2821 gacctcttac gtgcgccttt ctgccggacg cgagcagatg aacgaacaga ctcaggcgat
     2881 gtgctttatg gcaggcgcaa actcgatttt ctacggttgc aaactgctga ccacgccgaa
     2941 tccggaagaa gataaagacc tgcaactgtt ccgcaaactg gggctaaatc cgcagcaaac
     3001 tgccgtgctg gcaggggata acgaacaaca gcaacgtctt gaacaggcgc tgatgacccc
     3061 ggacaccgac gaatattaca acgcggcagc attatgagct ggcaggagaa aatcaacgcg
     3121 gcgctcgatg cgcggcgtgc tgccgatgcc ctgcgtcgcc gttatccagt ggcgcaagga
     3181 gccggacgct ggctggtggc ggatgatcgc cagtatctga acttttccag taacgattat
     3241 ttaggtttaa gccatcatcc gcaaattatc cgtgcctggc agcagggtgc ggagcaattt
     3301 ggcgtcggta gcggcggttc cggtcacgtc agcggttata gcgttgcgca tcaggcgctg
     3361 gaagaagaac tggccgagtg gctgggctat tcgcgggcac tgctatttat ctctggtttt
     3421 gccgctaatc aggcggtcat tgccgcgatg atggcaaaag aggaccgcat tgtcgctgac
     3481 cggcttagcc atgcctcatt gctggaggct gccagtttaa gcccgtcgca gcttcgccgt
     3541 tttgttcata acgatgtcac tcatctggcg cgactgcttg cttccccctg tccggggcag
     3601 caactggtgg tgacagaagg cgtgttcagc atggacggcg atattgcgcc actggcggaa
     3661 atccagcagg taacgcaaca gcacaatggc tggttgatgg tcgatgatgc ccacggcacg
     3721 ggcgttatcg gggagcaggg gcgcggcagc tgctggctgc aaaaggtaaa accagaattg
     3781 ctggtggtga cttttggcaa aggatttggc gtcagtgggg ctgcggtgct ttgctccagt
     3841 acggtggcgg attatctgct gcaattcgcc cgccacctta tctacagcac cagtatgccg
     3901 cccgctcagg cgcaggcatt acgtgcgtca ctggcggtca ttcgccgtga tgagggggat
     3961 gcacggcgcg aaaaactggt gtcactcatt gcgcgttttc gtgccggagt acaggatttg
     4021 ccgtttacgc tggctgattc atgcagcgcc atccagccat tgatcgtcgg tgataacagc
     4081 cgtgcgttac aactggcaga aaaactgcgc cagcaaggct gctgggtcac ggcgattcgc
     4141 ccgccaaccg tacccgctgg tactgcgcga ctgcgcttaa cgctaaccgc agcgcatgaa
     4201 atgcaggata tcgaccgtct gctggaggtg ctgcatggca acggttaata aacaagccat
     4261 tgcagcggca tttggtcggg cagccgcact ctatgagcaa cacgcagatc tacagcgcca
     4321 gagtgctgac gtcttactgg caatgctccc acagcgtaaa tacacccacg tactggacgc
     4381 gggttgtgga cctggctgga tgagccgcca ctggcgggaa cgtcacgcgc aggtgacggc
     4441 cttagatctc tcgccgccaa tgcttgttca ggcacgccag aaggatgccg cagaccatta
     4501 tctggcggga gatatcgaat ccctgccgtt agcgactgcg acgttcgatc ttgcatggag
     4561 caatctcgca gtgcagtggt gcggtaattt atccacggca ctccgcgagc tgtatcgggt
     4621 ggtgcgcccc ggaggcgtgg tggcgtttac cacgctggtg cagggatcgt tacccgaact
     4681 gcatcaggcg tggcaggcgg tggacgagcg tccgcatgct aatcgctttt taccgccaga
     4741 tgaaatcgaa cagtcgctga acggcgtgca ttatcaacat catattcagc ccatcacact
     4801 atggtttgat gatgcgctca gtgccatgcg ttcgctgaaa ggcatcggtg ctacgcatct
     4861 tcatgaaggg cgcgacccgc gaatattaac gcgttcgcaa ttgcagcgat tgcaactggc
     4921 ctggccgcaa caacaggggc gatatcctct gacgtatcat ctttttttgg gagtgattgc
     4981 tcgtgagtaa acgttatttt gtcaccggaa cggataccga agtggggaaa accgtcgcca
     5041 gttgtgcact tttacaagcc gcgaaggcag taggttatcg gacggcaggt tataaaccgg
     5101 tcgcctctgg cagcgaaaag accccggaag gcttacgtaa tagtgacgcg ctggcgttac
     5161 agcacaacag cagcctgcag ctggattacg caacggtaaa tccttacacc ttcgcagaac
     5221 ccacctcgcc gcacatcatc agcgcgcaag agggcaggcc gatagaatca ttggtaatga
     5281 gcgccggatt acgcgcgctt gaacaacagg ctgactgggt gttagtggaa ggtgctggcg
     5341 gctggtttac gccgctttct gacactttca cttttgcaga ttgggtaaca caggaacaac
     5401 tgccggtgat actggtagtt ggtgtgaaac tcggctgtat taatcacgcg atgttgactg
     5461 cgcaggcaat acaacacgcc ggactgactc tggcgggttg ggtggcgaac gatgttacgc
     5521 ctccgggaaa acgtcacgct gaatatatga ccacgctcac ccgcatgatt cccgcgccgc
     5581 tgctgggaga gatcccctgg cttgcagaaa atccagaaaa tgcggcaacc ggaaagtaca
     5641 taaaccttgc cttgttgtag ctatcctgta tttggt
//
LOCUS       DAZGJW010000230         5596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZGJW010000230 DAZGJW010000000
VERSION     DAZGJW010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.232"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(223..816)
                     /gene="tehB"
                     /locus_tag="ACHKIK_004756"
     CDS             complement(223..816)
                     /gene="tehB"
                     /locus_tag="ACHKIK_004756"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO9085768.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(813..1805)
                     /gene="tehA"
                     /locus_tag="ACHKIK_004757"
     CDS             complement(813..1805)
                     /gene="tehA"
                     /locus_tag="ACHKIK_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO9085769.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            1929..2909
                     /gene="ydcK"
                     /locus_tag="ACHKIK_004758"
     CDS             1929..2909
                     /gene="ydcK"
                     /locus_tag="ACHKIK_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO9085770.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDGVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(2904..3440)
                     /gene="rimL"
                     /locus_tag="ACHKIK_004759"
     CDS             complement(2904..3440)
                     /gene="rimL"
                     /locus_tag="ACHKIK_004759"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO9085771.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(3503..3727)
                     /locus_tag="ACHKIK_004760"
     CDS             complement(3503..3727)
                     /locus_tag="ACHKIK_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO9085772.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(3867..5522)
                     /gene="opgD"
                     /locus_tag="ACHKIK_004761"
                     /gene_synonym="mdoD"
     CDS             complement(3867..5522)
                     /gene="opgD"
                     /locus_tag="ACHKIK_004761"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO9085773.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
BASE COUNT         1503 a         1377 c         1311 g         1405 t
ORIGIN      
        1 ggtgaaaata aatttttcag ccccttttca gacgacatag tcaatgccga aaaaggggct
       61 aagtcaatca tcagtagtta aatttcagta agttaagctg ctaaaaaact tcgactactg
      121 tgattaattt cacatccctt cgataaaaaa tatgcttacc gaaaagaaga acagtaaatc
      181 atctcagcgt ggggaatttt cacgcgcctt attcttagcg ggtcattttt tacgtgccag
      241 catcgtggcg aaacgcagtt taatacgatt accgttggcg tcggtgcggt gcagctcgcc
      301 gacgtcttca ttgtatttca ccctctccca gccttcgtaa tatcgacgta attctccctc
      361 tttgaaggca aacgggaagc cgacggtaca tggataatca gcggtatcca tcgccgccac
      421 aatcaggttg taaccaccag gtttagtgca acgttgcata ttggcaatca gccctgggat
      481 ggttttagcc tcaaggaaca tcagtaccac agtcgaaaga ataaaatcgt actgtctatc
      541 aaatgtgagg ttattcagat caacgactcg ggtgtgtaaa ttatccagat tttcaatgga
      601 tttaatgcgc tctacattgg cgatactcat ggcattttta tcccatgcgt caacatcata
      661 accattggct gccaggtaaa gactgttacg accattgcca cagcccagat ccagcgtttt
      721 acccggttta accactttca ccgcttccag tacttcagag tgtgtgcggg ttaattcata
      781 tttatcagta aaatagtttt cgtcacgaat gatcattctt tgtcctctgc tttcattaaa
      841 acggcgcgct cggttctgac taacaatttt ccctgcatca gaagcgcaaa agtacggatg
      901 agcagtattg caataataaa attggtaaag ataaacagcg gtaccgccag cgtatggaaa
      961 aatccatttt cgctgccact ccccagatgc aaaccggtgg ttgccagtgc agatacgccg
     1021 aacgagaaac tccagaatga agcattaaat ggctgggaga gataccatgg catcagacgt
     1081 agcataaaca gcagttgcag cagtccataa ccaaaaagca ttttcgccag cgtatcaccc
     1141 tcgccgccgt tgacgctcaa ccaggcacta caggccacca gcgcaggagc gagctgaatg
     1201 ccgagtgatg tccgcagtgc cgtgggtaat tctcccgaac tgcgcagacg ctgcaagatc
     1261 acaggttcaa ggcttagcca tgagaaaacg cctgcgccta aaaacaccag accggcgtcg
     1321 gtgtagccca acgcaccaca ggccattgcg ctgataaagt tgttggcaac tgtcggcaga
     1381 tacagtccag gcgtggtagc ttcttcaggg tgagatccgc gccataatcc cgcagtttgc
     1441 caggcggcat aagccaactg aaccacgaca ccaaaactga acaggcacac cgccagtggg
     1501 cgaaaccacg gaacaaaacc aatcgccacc agcatcgtcg ttgccggaaa caaactcaca
     1561 aaactgctca gcactggatg gcgaacttcc gccagcacgc tatgcggaaa gcgtatgagt
     1621 cgggtaataa atgcgctagt taataatccc cagatgatca tcgccagaat caccagccca
     1681 tcccctaacc agtggctgac ctgccaaacc tggctggcat agcgccaggc aaatcccatc
     1741 cctatcgtcc ccaacacaat accaaagtag cctgccggca aattgagcac tttatcgctc
     1801 tgcatcttct tactcatttt gtttaattta taaagtatat ttgaaatgca ttttatggaa
     1861 ttttctcgag catagccaga gccgcagaat ttgctacggt tatagtaagt cacctgtggg
     1921 aaaacgctat gcgcaaatat cgtctaagtg aagaacagcg agcctttagt tatcaggaag
     1981 atggcactaa aaaaaatgtg ttattacggc agattatcgc cattagcgat tttaacgatg
     2041 tgatagcagg aaccgctggc ggctggatcg atcgcgaaac ggtgttagca caggaaggaa
     2101 attgctggat ttacgaccag aatgctattg cgttcggcgg aacggtgatt tccggtaata
     2161 cgcgcatcac cggcaccagc gtactgtggg gagaggttta cgcaacggat aatgtctgga
     2221 tcgataatag tgaaatcagc cagggtgcat atataagtga cggtgtcacc attcatgact
     2281 cactggtttg cgggcaatgt agaatatttg gtcacgcctt aattaatcaa cattctatga
     2341 tcgtcgcagc acaaggctta acgcctgacc atcaactcct gctacaaatt tatgatcgcg
     2401 caagggttag cgcctcgcgt attgtccacc aggcacaaat ttatggcgat gccgtcatcc
     2461 ggtatgcttt tattgaacat cgtgccgaag tttttgattt tgccagtatt gaaggtaatg
     2521 aagagaacaa cgtctggcta tgcgattgcg ctaaagtcta cggtcacgcg caggtgaaag
     2581 ctggcataga agaagatgcc attccaacga ttcattacag ttcgcaggtg gcggaatatg
     2641 ccattgtgga aggtaattgt gtgttaaaac atcatgtctt gattggcgga aatgccgtgg
     2701 tacgtggtgg accgattctg ctcgacgagc acgttgtaat acaaggtgag agccgtatta
     2761 caggtgcggt gataattgaa aaccatgtgg agttaaccga ccacgccgtc gttgaagcct
     2821 ttgatggcga cacggttcat gtccgtggtc caaaagtcat taacggcgag gagcgcatta
     2881 cgcgaacgcc gctggcaggg ttattatgaa tcgataatac gcgcgtataa gttcacatca
     2941 tcataggcat cattcaggaa ctcagcctgt ttcaggcaac cttcaaggat aaaaccattg
     3001 cgcaaagcga cctggttgct ttgcggattg tccacccgac atttgatcac gaagcgtcta
     3061 agttcaccag actgggcgta atgatgaatc aatgcctgca gcgcctgaga aatgatcccc
     3121 tgcccctgat gagattcgtc cagccagtag cctatttcag cggttttatt cagtggttca
     3181 atacgattaa acgagataac gccgataagt tcatcttctt tgaaaatcat gaacattttg
     3241 gcatagccgc gttgatgcaa catcacatta ccctgcaccg tttttcgcgt gtcctcttca
     3301 ctctgaacga actgcggcca gtttagcgac tgctgtaacc aggttttatt tttacagatt
     3361 aactgataaa gaggagtgac gtgattttct gcaacagcat gtaattcaag tgattcgctt
     3421 acttttatcg tttcagtcat cttatctcca gtcaatccat gcagttgtta aaaaagccgc
     3481 ctggctggcg gcttaggaaa gtttacacct ctttgacgct ctcctgctgc aggattttga
     3541 gcatctcatc ttttaactgt agcttttgtt ttttcatgcg gaccacttcc gcattgtacc
     3601 ctcgaccgtc ggaaccttcc tttctggcaa tttcatgatc aagtttattg tgtttatcga
     3661 acaaggacat aaagcgagga ttttcgtttt tcagacggga tattaaatct cgatattctg
     3721 gaaacatacg cactccctgt tagtggctga caaggtgtat ctgaaataca caccatcaga
     3781 ttactcattt aacttaggga aaaatgcgag cgggatcgtg tttaaccaga cggcacctcc
     3841 cgaagaaggt gccgaagaaa aaacgattaa ctcatcacgc ggtcgtcaac atactgacgt
     3901 ttatccggcg ctggcgggaa atactgatac agccatgttt cactgatagc gtcgccctga
     3961 caacgcagat acatccgcat atcgaccgga tcagtggagt ccgaagtcgg ataccagtca
     4021 aactgaatac gataaccatc aatgggttca atatagagaa tttcgatttg cttcgcttcc
     4081 ccactggaaa gcgtaatcac cggctcaatg ccttttggtg cggcagcttt cagatcacca
     4141 ccaacgaaat cgacggcaaa acgacgcgcc catttttcgg gatagtgttc acctggtgcc
     4201 caaccttccg ggaaaccgcc catgccggta cgcgtcgcca taacgcgcgc taatgggcaa
     4261 tgaacaggcg gttgcgcact ccagtacaga cgatactgga atgcaaactc atcacccgcc
     4321 tttacagctt tttctggctg ccagaagcag acaatgttat ccagcgtttc gcccgttgtt
     4381 gggatttcca tcaggccgat ggtgccctta ccccacttgt tacgcggttc cacccacaga
     4441 cttgggcgtt tgttatacca gcccataatg tcctgataat gggagaaatc acgatccagt
     4501 tgcaataaac caaacccttt cgggttgttg tcggtgtaag cattgaactg caatttttgc
     4561 ggattattca gcggacggca aatccactcg ccgttgcccc gccacatgga cagacgatca
     4621 gagtcatgaa tttgcggatg aatggtgtcg cacatccgac gttcattagt accgcagctg
     4681 aacatactgg tcatcggcgc aatgcccagc tgtttaatgt ctttgcgcgc atacaggtga
     4741 ttttccacat ccataatcac ctgacttttc tcacaatgga tagtgaactt ataggcacca
     4801 gtaatgctgg cgctatcgag caacgcataa acggtaaagg tagttgcccc cggttttacc
     4861 gtatcaaacc agaaggcggt aaagtcgggg aactcttctt tactgtcggt gtaagtgtca
     4921 atcgccaggc cgcgggccga caaaccgtat tgatatgtgt catcaacggc gcggaaataa
     4981 ctcgctccga ggaatgatac tacatcacgg cgcgccagtt cgggggcttt aaacacgcga
     5041 aaaccggcaa agccgagatc gctttgccct tctaattgtt tggtatcaac acctgcatcg
     5101 ttgtatttga acaactccgg gcgaaagtga atttcacgcg ccagatgtgt tgctggatct
     5161 acagaaaaca tacgaacgcg gcgacggaat cccattccca tatggaagaa ctgagcgtcc
     5221 agttgacggt tctcaacgtt atgccagagc gatttttcgg cgtcgtattg aatactgtta
     5281 taagcctgcg gcgtcattgt cgccagcgtg tcaggtaacg gacgaggcgc accacgccac
     5341 gctgtttgcg ctaagtcgtg cgccattgac tgtagaatgg agaagtcaaa acgctgggtt
     5401 tgcccgtcgg caatatcaga atctgccgcg aatgccgcct gagaaaaaag agaagcaatg
     5461 ccgctggtac cgcacacggc ggccatagcc attgaacctt taataaatcg tctacgatcc
     5521 atacctgaaa gtgagtcctt ctggtgttgt gaatggtccc gcgctggtcg ttttcagcgg
     5581 caagaaagtg cttatt
//
LOCUS       DAZGJW010000231         5547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZGJW010000231 DAZGJW010000000
VERSION     DAZGJW010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.233"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            126..1484
                     /gene="mltD"
                     /locus_tag="ACHKIK_004762"
     CDS             126..1484
                     /gene="mltD"
                     /locus_tag="ACHKIK_004762"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO9085774.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1532..2302)
                     /locus_tag="ACHKIK_004763"
     CDS             complement(1532..2302)
                     /locus_tag="ACHKIK_004763"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO9085775.1"
                     /translation="MTIQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVVRMRTPEALID
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2380..3180)
                     /locus_tag="ACHKIK_004764"
     CDS             complement(2380..3180)
                     /locus_tag="ACHKIK_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO9085776.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3421..4335
                     /gene="yafC"
                     /locus_tag="ACHKIK_004765"
     CDS             3421..4335
                     /gene="yafC"
                     /locus_tag="ACHKIK_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO9085777.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGQRYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIVRGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4332..5135)
                     /gene="dkgB"
                     /locus_tag="ACHKIK_004766"
     CDS             complement(4332..5135)
                     /gene="dkgB"
                     /locus_tag="ACHKIK_004766"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO9085778.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAVIGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5298..5374)
                     /locus_tag="ACHKIK_004767"
     tRNA            complement(5298..5374)
                     /locus_tag="ACHKIK_004767"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5338..5340),aa:Asp,seq:gtc)
BASE COUNT         1450 a         1340 c         1370 g         1387 t
ORIGIN      
        1 caacaacctg aagagcgttt tgcatggtta aggtcaaaga aagataggtt ctgataaaac
       61 tttcttgtca tcggctccgt tcgccgttat gatcggtcgt cttttaagca actattgaca
      121 cacacatgaa ggcaaaagcg atattactcg cctctgtcct gctcgtgggt tgccagagta
      181 ccggcaacgt tcaacagcac gcacagagcc tttctgcagc tggtcaaggg gaagcagcaa
      241 agtttacaag tcaggcacga tggatggacg atgggacgtc tatcgcgcca gatggtgact
      301 tgtgggcttt cattggcgac gagctaaaga tgggaattcc ggaaaatgac cggattcgcg
      361 aacagaaaca gaaatattta cgcaataaga gctatctcca cgatgtaact ttacgggcag
      421 agccgtatat gtactggata gcagggcaag ttaaaaaacg taacatgcct atggaactgg
      481 tactactacc catagtggag agcgcttttg atcctcacgc aacgtctggc gccaatgccg
      541 caggcatctg gcagatcatt ccgagcacgg ggcgcaatta tggtttgaaa cagacccgca
      601 attatgacgc gcgtcgcgat gttgttgctt caacaactgc cgcgctgaat atgatgcagc
      661 gtctgaacaa gatgtttgac ggcgactggc ttctgaccgt agcggcttat aacagcggcg
      721 aaggtcgagt catgaaggca attaaaacga acaaagcgcg tgggaaatcc acggacttct
      781 ggtcgttacc gttgccgcag gaaacgaagc agtacgtgcc taaaatgctg gcattgagtg
      841 atattctcaa aaacagcaag cgttatggcg tacgtctgcc aacgaccgat gaaagccgtg
      901 ctctggcgcg tgtgcacctg agcagcccgg ttgaaatggc gaaggttgca gatatggcgg
      961 ggatttccgt cagcaagctg aagacattca acgctggcgt gaaaggctcc acgctgggcg
     1021 caagtggtcc gcagtacgtg atggtgccaa agaagcatgc agatcaactg cgtgaatctc
     1081 tggcttcagg cgaaattgct gctgtacagt cgacgctggt tgccgacaat acgccgctta
     1141 acagccgtgt ttacaccgta cgctctggcg acacgctttc aagtatcgct tcacgtctcg
     1201 gcgtaagcac caaagatttg cagcagtgga acaaactgcg cggatctaaa ctgaagccag
     1261 gccaaagttt gacgattggc gcaggcagta gcgcacagcg actggcaaac aacagcgata
     1321 gcattacgta tcgtgtgcgc aaaggcgatt cgctttcaag cattgctaaa cgccacggcg
     1381 tgaacatcaa agatgtgatg cgctggaaca gcgacactgc gaatctgcaa ccaggcgata
     1441 agctgacgtt gtttgtgaaa aacaacaaca tgccagattc ctgacaaacc agataataaa
     1501 aaggcaccga ttcccccggt gcctttttta tttatgccgc tttatgtgct tctaccatga
     1561 tgatatcact ggtgaaagag ccgtcattct gcaaggcaaa atacgttctc acctctgtcg
     1621 atgcgctctg ttggtaaatg cgaatagcgt ctattaacgc ttctggcgta cgcattctca
     1681 cgacccatga agaaaattcc agcggtaact tatctgtaat taaattatca actatcagat
     1741 tggcttcatt gattaacgtc aaccactcac cgctggcgta gtttcgtacg tgagaggtat
     1801 cgcgtaatgc ttctaccgtc tgtaaccaga tgtcgcgcac tgggtgaccc ggagacatta
     1861 cgtccatcac aatcagccta ccgccaggtt tcaatatccg gttcacttca cgcagtgctg
     1921 caccaacatc atgccagtga tgggcagaat aacggctgat aacaatatca aatgcgttgt
     1981 cggcaaatgg cagactttcg gcataaccct ggcgggtggt gatatttttc agttgccgga
     2041 cttcggcagc ttgtgccacg acatctagca tgtgggcaga taagtcatac gccaccaccg
     2101 tgctcacgtt ttgcgcagcg acaaagctgg catgccctgc tccgcagccc atatcaagta
     2161 cacttgcacc aggataatca gccagacgca ccgccaggcg ctgtaaatct cgtccggatg
     2221 catgtacggt actggtcaga tattcgctgg cttgagagct aaactgcttt tctacatggt
     2281 catggtggga ttgtattgtc attgcgctgt ccttttgtta tttgagtata aaagggcagc
     2341 gccaaacgtt ggtctgccct gtggcagacc tgacatacct tatttatcag gcttgccggg
     2401 actgaattca acgagtagcg gattgtgatc ggaagcgcgc gtaaccagta cagaagcttc
     2461 gctgacgttc agaccacggt agaaaacaaa atcgagcggg cgaccaaacg cccgacggcg
     2521 ctgatcatcg gtaaaacgca cctggcgcag cgacatttcc cgcgcaaagc gatataacgc
     2581 gttcatcctt ctacggctcc aggcattgaa atctcccgcc ataatgaccg ggccgctgtg
     2641 gtgagctatc tgatcaccaa taggaagtaa ctgcttacta tagacatcca cgcccagact
     2701 gaagttgacg gcgtgtatat taaccaccat caacaggcgg gtgtcaggta atggatagac
     2761 cgtcaccagt gccgacttcg ccagacgcaa aatgggttct cgttcacgta acgggcagca
     2821 atacactgga tgcgccgccg aaagggtcat tacaccggaa ggatgttgtg gcagcacgaa
     2881 agcgggcacc tgatcggcgg caagatagtt agcggtcgca aactgtacta actctggcgt
     2941 tgtctgcgct tcctgcaata acaccagatg tgcatctttg ccgtagttct ttaataccga
     3001 caaccattca gcgcgttgct gtttgtatat gttccacacc aggatccgaa tacgctcttc
     3061 agtacttaac ggttctccag gtggtaatgc ctggccgatg ctcgcaaaag accccggcgg
     3121 taagatcctt tccgcaggtt gtccggcaac atagcgcatg gcataggtgt tttttcgcac
     3181 ttttgacttt gaaacctcta ttacgtcaac ccttccgcac tcgggagggt tctactgtta
     3241 tagggatttt agctcactct ttcaacgagc aattgtatat tgttatgtaa gcaagtgctt
     3301 acacaggtca gaattttgct cattattgag aacattaaat cggatgacat atcacgcatc
     3361 ctgatggttt aatgacaaat aaattcaaaa tatctcttct attttgcaac aggagcaaaa
     3421 atgaaagcca cgtcggaaga actcgccatt tttgtttcgg tcgtagaaag cggcagcttt
     3481 agccgggcag cggaacaatt agggcaagca aactcagcgg taagccgggc agtgaaaaag
     3541 ctggagatga aacttggcgt tagcctgctt aatcggacca cgcgacaact tagcctgacg
     3601 gaagaaggcc agcgttattt tcgtcgcgta cagtcaattt tgcaggagat ggcagcggca
     3661 gaatcagaaa ttatggagac gcgtaataca ccgcgtggac tgttacgcat cgatgccgca
     3721 actccagtgg tgctgcactt tctgatgccg ttaattaagc ctttccgtga acgctatccg
     3781 gaagttactt tatcgctagt ctcctccgaa acgattatta atttgatcga aagaaaagtg
     3841 gatgtcgcga tacgcgctgg tacgttaacg gattccagct tacgtgccag gccgttattt
     3901 aacagttatc gaaaaattat cgcctccccc gattatattt cccgctacgg gaagccagaa
     3961 acgatcgacg atttaaagca acatgtttgc ctgggattca ctgaacccgc ttccctcaat
     4021 acctggccga tagcctgtag tgatggacaa ttacatgagg tgaagtacgg tttgtcatcc
     4081 aatagtgggg aaacactgaa acagctttgc ctgagtggca acgggattgc gtgtttgtcc
     4141 gactacatga tcgacaaaga aatcgttcgc ggagaattgg tggagttaat ggcagataaa
     4201 gtgttgccag tggaaatgcc attcagtgca gtctattaca gcgaccgtgc ggtaagtacg
     4261 cgcatccggg cttttatcga tttccttagc gagcatgtaa aaacagctcc cggaggagct
     4321 gtcagagagg cttaatccca ttcaggagcc agaccttccg ggctaaccag gcggtcgttg
     4381 caatccagtg cggcgatcgc ttttttatct tcggcatcaa gctgtaaatt ttgtgcctta
     4441 agattacttt ccaggttttc acgtttagta gaagaaggaa ttactgagta accttccccc
     4501 atagcccacg ccagaatcac ttgtgccgga gtcgcattgt gtttagctgc gatacgagca
     4561 ataacctcat ctttcaaggc tttcccataa gccagcgtca tataggaagt aatatggatg
     4621 ccgtgctgtt tagcccaggc aaccacttta cggttttgca gataaggaga gagttcaatc
     4681 tggttagtag cgatgttttc agcgccaata acagcaatcg ccttttccat caatgggatc
     4741 gtgaagttgg aaataccgat ctcacgcgtc agcccttgtt ttttggcttc cagcagcgcc
     4801 tgcataaact cttcaacaga gacttcatcg tttggtgacg gccagtggat tagcgtcaga
     4861 tcaacataat cggtacgcaa tttttgcagg ctctctttca gacttgggat caatttgtct
     4921 ttgctgagat tttcaatcca gattttagtg gtgatgtaga gttcatgacg tggcacgcca
     4981 ctttctgcaa tcgcctgacc tactgcggct tcgttatcat agatttgtgc ggtatcaatt
     5041 gcgcgataac caagttcaag cgccgttttc acagatgaaa taacaacgtc gtctttcaga
     5101 cggaaagtac ctaaaccaaa tgcagggata gccataatat gcctctttta atgctatttt
     5161 cgttaactga ggtgattatg ggcttcagac tgttgaggaa aaagagcgta aaatgcagag
     5221 gatttttgcg attctggcaa taatagatat ccagacgaaa aaaaacctcg agcgttaact
     5281 cgaggcttct taataagtgg cggaacggac gggactcgaa cccgcgaccc cctgcgtgac
     5341 aggcaggtat tctaaccgac tgaactaccg ctccaccgaa ttcttttaca accaccggtt
     5401 ttatgaccgg cttactgctt aatttgatgc ctggcagttt atgaatcact tcgtgattca
     5461 cccttcgggc cgttgcttcg caacgttcaa aagctttcgc ttttgtccta ctctcttatc
     5521 gagtagtgaa ctgcctcaaa gatcagt
//
LOCUS       DAZGJW010000232         5511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZGJW010000232 DAZGJW010000000
VERSION     DAZGJW010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.234"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..670
                     /locus_tag="ACHKIK_004768"
     CDS             2..670
                     /locus_tag="ACHKIK_004768"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002443700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO9085779.1"
                     /translation="MAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQAGNVGRAV
                     TKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRRGLIV
                     LTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDL
                     EVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAIMDFMTA
                     RGYH"
     gene            complement(719..2218)
                     /gene="pitB"
                     /locus_tag="ACHKIK_004769"
     CDS             complement(719..2218)
                     /gene="pitB"
                     /locus_tag="ACHKIK_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO9085780.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVSNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(2510..4369)
                     /gene="gss"
                     /locus_tag="ACHKIK_004770"
     CDS             complement(2510..4369)
                     /gene="gss"
                     /locus_tag="ACHKIK_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO9085781.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQEGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNINLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            4574..5440
                     /gene="yghU"
                     /locus_tag="ACHKIK_004771"
     CDS             4574..5440
                     /gene="yghU"
                     /locus_tag="ACHKIK_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO9085782.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
BASE COUNT         1403 a         1434 c         1352 g         1322 t
ORIGIN      
        1 aattgccgtt attggtagcg atggttcagg caagtcaacg gtgtgtgaac atcttattac
       61 cgttgtcgaa aaatatggtg ctgccgaaag agttcattta ggaaaacaag ccggaaatgt
      121 cggtcgtgca gtgacaaaat taccgttgat gggaaaatcc ttacataaaa caattgaacg
      181 aaatcaggtg aaaacagcaa aaaaattgcc aggaccagtt ccggcgctgg taattacagc
      241 gtttgtcgcc cgtcgcttac tgcgctttcg tcatatgctt gcctgtcgtc gtcgcgggtt
      301 aattgttcta accgaccgtt atcctcagga ccaaattcct ggcgcttacg atggtacggt
      361 gttcccacct aacgttgaag gtggtcgttt tgtctcatgg ctggcaagcc aggaacgtaa
      421 agcgtttcac tggatggcga gccataagcc tgatctggtc atcaaactca atgttgacct
      481 tgaagttgcc tgcgcgcgta agcctgacca caaaagggag tcgctggcaa gaaagatagc
      541 cataacgcca cagttaacct ttggtggtgc acaactggtt gatatcgatg ccaatcagcc
      601 actggaacag gtgttggttg atgcagaaaa agcgattatg gattttatga ccgcgcgtgg
      661 ttatcactag ccaaaaatgg aaatgcccga tcgccaggac cgggcatttt caggaaggtt
      721 aaatcaactg caatgctatc cagtacagcc caccagaaag aaaaatcgcc gccggtaaag
      781 taaataccca cgccatcagg atgctggtta ccgttttacg ctgtaaccca ccgccgtcca
      841 ccaccatcgt ccctgcaact gcagacgaga ggacgtgtgt tgtggagacg ggcatcccaa
      901 tataactggc aagaccgata gacactgccg ccgtcatttg tgccgccatg ccttgcgcat
      961 acgtcatgcc gcgcttacca atcttctcac cgatggtcat cgctacacga cgccagccaa
     1021 tcatggtgcc aatgccgagc gccagtgcta ccgccatgat gatccacacc ggagcgtact
     1081 caatggtgct taacatatcg ctgcgcagtt ttttcagcag gttctggtct tctttactga
     1141 cgcctggcag tttcgccagc ttcgcggagg tatctgagat gcacagcata atgcggcgca
     1201 gctggctgcg ctgactcacg cttaacggct cgtaactttc catattgcct ggcagcatcg
     1261 ttttaacgcg cgcaatcgca tcaaaggtat ttgccggatg acagtgaaac tctgttactt
     1321 gcgcgccatc agtcgatgcc gcaggcaatg gaggttccat cgcaatcaac ttctgcggca
     1381 gttcaggatg ctgttgcaga tagtgttcga agttggagac ggcatcgcga gtacgggtaa
     1441 tttcatagcc ggacgcgttc atattgacga cgaagccagc aggggcaatc cccaccagta
     1501 ccagcatcac caggccgatc cctttctgcc cgtcgttcgc gccgtgcgaa aacgccacgc
     1561 ccgcagcgga aacaatcagc gcaatacgcg tccagaatgg cggtttacgt ttgccttttt
     1621 tctttttgcg atcttccgga atgcggtgaa tacggtcacg ctttttcgtc ccgctccaga
     1681 aacggcgcag caggaaaatc agaccacccg caatgaccag gccgacgata ggggaaacaa
     1741 tcagcgagga gaaaattttg gtcacttcac gcaggtttaa cgcatccatc accgatgagc
     1801 cggttaacag cgcgttggtt aaaccgatgc cgataatcgc gccaatcaag gtgtgcgaac
     1861 tggaggccgg taaaccgaag aaccacgtcc ccaggttcca tataatcgcc gccagcagca
     1921 tggaaaagac catcgccaga ccgtgggttg accccatatt cagcaacaaa tcggttggta
     1981 acatatggac aatggcatag gcaacgctaa gtccgcccaa taacacgcca aaaaagttaa
     2041 aaaatgccgc catcaccaca gcaagttgtg gttgcatggc acgagtataa ataacggttg
     2101 ccaccgcatt cgccgtgtca tgaaaaccat tgattgcttc gtagaacaac acaaatgcca
     2161 gagcaagcaa taacaaaagc cctgtgtata tatcaaggcc aacaaataaa tttagcatat
     2221 attagattac gccattttga atttacgaac ggacgcatta tcagtgactt taacggcatg
     2281 ggcaaagtga aatatcattt tttaacgata aaaagagtgt tatttcttgt gtatcagaca
     2341 ataagcattc attcaaaacc tttagaaata atcaaaacac aggtattcca ctggttaata
     2401 tattcacata tgaaatgaat aaatattgga atatataaat attgaatatt ttgattaatc
     2461 cccgtactaa ttattcttca taatcagtac ggggttgcta caacatgtat tactttttca
     2521 ccacaattaa cggttcaata tcactctctt ttttgatgac cagtgattca tcaccgcgca
     2581 aacacgtccc accgtagttg ccgccaacgg tgaaggtaca tacctgaatg tatttaccgt
     2641 ccactttcgg caaacaccac agttgctgat agatgttttt ctgctcggca aatttaccgc
     2701 tggttttgtc cagcacctct tcatgatggc tgaccaggtt gatattgcta ccacagcgac
     2761 cggcaatcgg tttcactgcg taacctgttt tcaccaattc atcattaaca gtgaaatcgg
     2821 tatccagcag gtaacgatgg tgcgggaaca gcgaccagag gatcggcaga atcgctttgt
     2881 tgccggggat caccgtccac agcggctcaa agactagcac ttccgggcgc agcaatacgt
     2941 cgataagacg cacttcgttt tgcggatgac cggtacggat tggcaccgca gcaaactcac
     3001 ggtcgctaac ttcacgaatc tgatcaaacg cggtttccca cgcccaggtt ttccacacgc
     3061 agttaaccag ccgtccttcc ccatcaatca gttgcccggc agcatcccag cccagttcat
     3121 ccagcccgcg caagatacgc gtttcaaagc cctcctggtg cagcgcctgc tccataaact
     3181 gcgcgtgata gttttcctcg atatctttgt cctgcatgat atggacaaac ggacgtgcac
     3241 gactgtgttt ccaggcacca gccagttcgt taatcagccc ttccgccgga ttgaagccgt
     3301 tgcctttata gccctgctcc gcccaacgtt cgaggatcaa gcccgcttcg gtatgacatg
     3361 aggcagagtc ggcgttgtat tcgtaaacct tcaggccacg ctcatccatg cagaaatcca
     3421 tacgaccagt gatcatatgg tgacggcgac gctgccagga gagacgcaaa cgtggccaga
     3481 ggattttcgg gatgtcgaac agcgccagca ggttgtcatc tttcagcacc ttgtcggttg
     3541 cgtgaagata catcaggtgc agctcgttgg tggctttaat tagctcctgc tcggcactct
     3601 cggtaatggt gtagtaatga taaggatcct gattgatcac ctgaccgttg gcctgcacat
     3661 aggcgttttg cagcggatct ttttcatcca gccatttacc gtcaaactgg cctttgtttt
     3721 ccaggcgcgc tccgctgatt ttcagcagct cgcctgcaat ttccggctgc ggtaagctgt
     3781 attcagtatc ttccgtctgg atcatccagc ccagaatggt ggtgtcatca aaagtgtctt
     3841 tcagggtata gcagccgttt tcgaccacca tctccagctc acgcgtccac tgttgccctt
     3901 gcggcaacgg ggaatgaatc acgttctgtt ccgcaatacg gactttgttg ccatgcaatt
     3961 gggtaatgat ggcgacatgg ccagtgtctt taaattcacc gcctttatcc cagataagaa
     4021 gcgcacccgc gaccggcgca cgcggcgagc cgttaggaaa tgcctgcaat ggcaggatgt
     4081 tgtcattaac cacttctcgc aggaagcgca gcgagaaaat ctcccacgcc atacccacgt
     4141 cagtaaagac cacaccgtaa ttcagaaaga gaaaacggcg agcaaattca acgcattgcc
     4201 acttgtggcc catatattcg tcgtcgatat agctacggaa tacggcgtca tcttcgtatt
     4261 cctgcggatc gagagaactg taatctgaag agtagattgc taccccacct ggggcgtagc
     4321 ccaataatgt cccgaacggg gcatcctggc tggtcgttcc tttgctcatc actttacctt
     4381 taacaataca acctaagcag ttggcgtaat tgttgtgctc tgattacctg ctcagcgaca
     4441 ttaaccggac agaggtcaga ctaatcatac acctcatcgc agcggctggc gcaaagggtt
     4501 aaaaaattca cattctgtac agcaagtgac ctgctacact gcttcaacac taccactcag
     4561 aaggcaactc actatgacag acaatactta tcagcccgcg aaagtctgga cgtgggataa
     4621 atccgctggc ggcgcgttcg ccaatatcaa tcgcccggtt tctggtccga cgcatgaaaa
     4681 aacgctgccc gttggcaaac acccattgca actttattcg ctgggaacgc cgaacggtca
     4741 gaaagtaacg attatgcttg aggagctgct ggcgctgggc gttactggtg cagagtacga
     4801 cgcctggctg attcgtattg gcgatggcga tcaattctcc agcggctttg tcgaagtgaa
     4861 cccaaactcg aagatcccgg cgctgcgcga tcatacgcat aatccgccga tccgcgtgtt
     4921 tgaatctggt tcgatcctgc tttatctggc ggagaaattt ggctacttcc tgccgcagga
     4981 tttggcaaag cgtactgaaa cgatgaactg gctgttctgg ttacagggcg cggcaccgtt
     5041 cctcggcggt ggttttggtc acttttacca ttacgcgccg atgaaaattg agtacgccat
     5101 caaccgcttt accatggaag ccaaacgtct gctcgacgtg ctggataagc aactggcgca
     5161 gcataagttt attgcgggcg atgagtacac cattgcggat atggcgattt ggccgtggtt
     5221 tggcaatgtg gtgttaggcg gcgtgtatga tgccgcagag tttcttgatg cgggcagtta
     5281 taagcatgta caacgctggg cgaaagaagt aggcgaacgt ccggcggtga aacgtgggcg
     5341 tattgttaac cgcaccaacg gaccgctgaa tgagcagttg catgagcgcc atgacgccag
     5401 tgatttcgag acgaatacgg aagataagcg tcaggggtaa gggttggtgt tcgtcgcagc
     5461 aagccatcca ggccggataa ggcgttcacg ccgcatccgg caatcgtgca c
//
LOCUS       DAZGJW010000233         5500 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZGJW010000233 DAZGJW010000000
VERSION     DAZGJW010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.235"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..369)
                     /locus_tag="ACHKIK_004772"
     CDS             complement(<1..369)
                     /locus_tag="ACHKIK_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9085783.1"
                     /translation="MQGATGLELYEAYNNGYPTTYGNIIHLKGMTAVGEGELLIGWSG
                     ISGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPEEFYPVGAPIPWPSDTVPSGYALM
                     QGQTFDKSAYPKLAVAYPSGV"
     gene            complement(934..1647)
                     /locus_tag="ACHKIK_004773"
                     /pseudo
     CDS             complement(934..1647)
                     /locus_tag="ACHKIK_004773"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(1650..2180)
                     /locus_tag="ACHKIK_004774"
     CDS             complement(1650..2180)
                     /locus_tag="ACHKIK_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO9085784.1"
                     /translation="MSNSLLPPSASSFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATHDGEVITIYPDD"
     gene            complement(2173..3069)
                     /locus_tag="ACHKIK_004775"
     CDS             complement(2173..3069)
                     /locus_tag="ACHKIK_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO9085785.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTTAFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLT
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            complement(3073..3423)
                     /locus_tag="ACHKIK_004776"
     CDS             complement(3073..3423)
                     /locus_tag="ACHKIK_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO9085786.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            complement(3420..3986)
                     /locus_tag="ACHKIK_004777"
     CDS             complement(3420..3986)
                     /locus_tag="ACHKIK_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO9085787.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWCVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDANPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            complement(3998..4633)
                     /locus_tag="ACHKIK_004778"
     CDS             complement(3998..4633)
                     /locus_tag="ACHKIK_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO9085788.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            complement(4626..5093)
                     /locus_tag="ACHKIK_004779"
     CDS             complement(4626..5093)
                     /locus_tag="ACHKIK_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9085789.1"
                     /translation="MLKTNSLRKGMIHGCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGAPQAEHLQEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            complement(5117..>5500)
                     /locus_tag="ACHKIK_004780"
     CDS             complement(5117..>5500)
                     /locus_tag="ACHKIK_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO9085790.1"
                     /translation="LGIYLYTDVALPDVVAMTGTGNPFLMSFFTQTTDGKLNLMHHKK
                     AGNTKLGEFGNYSNDWQTLELVFTASSATVTPKLNGVAGPAFQVIKDSLTLGLNALTL
                     TDITKNATYGVEIESLVLEINSPAA"
BASE COUNT         1101 a         1578 c         1454 g         1367 t
ORIGIN      
        1 aacgcctgac ggataagcga ctgcaagttt cgggtatgca gatttgtcaa aagtctgccc
       61 ctgcatcagg gcataaccag acggaacggt atctgatggc cacggaatcg gtgcaccgac
      121 tggataaaac tcttcaggag gatgagccga ggtgtaaagc tgcgcccacg gcgaccagtt
      181 tgcgtcggtc gtatcccgtc gtgaacgaat aaatgccgga gcatgagcac cgcttatacc
      241 actccagccg atgagtaatt cgccttcgcc aacggctgtc atccctttca ggtgaatgat
      301 atttccatac gttgttggat atccgttgtt atacgcctcg tataactcca gacctgtggc
      361 cccctgcata ttatctgtca gggcggtcag ccgacctttt gaagccagat taactgacga
      421 tactgctgtc ccatctgacg gtaacgcccc gatctctgat gctgttggct tattcctgga
      481 gttatagtcc cttcgccagc caggagcata agcatcacca tgattaatat aagtaaattg
      541 agcattaggg atgccttcac cgctggatgt actcggtgtg gtaatgcgta tggtcattgc
      601 gccgcgggtg ccaataactt ccacaacagc acctgcaaga caaatacttc cgcaacctgt
      661 atctgtaatg accttattat ttgcataagc ccatgagcct ttgcacatcc agtaaggatg
      721 gttaaatgcc ccctgactct ctagccaaaa aatcaactgc gcagttgtcc atgcttcact
      781 atctccacca atattcagct ctgcactata tgcacggcag gcaccgatat ttttcgtaaa
      841 ggtgtcttta tcagagatat cctcaccatt cttagctttc tgcagacgtt tttcagcatt
      901 gtcataggca gcttttactg catttgatgt tgccgccagc gtttcagatg tactgttggt
      961 tgcactacta agctggacaa ggccttttcg cgctgttgtg gcatcctgcg ctgtatattt
     1021 tccgttagca agatcatacg cgaccttaac cgctttcggc gttgcagcca gcgtttcaga
     1081 atcgctgtta gtggcgctac tgagttgaac aaagcctttt gcggtcagcg aggcatccgg
     1141 gtgacatcgt gactgttcat gctctttcag tttgttatcc acgtaatcca ctgtggccat
     1201 caccatggtg ttatccacgg taagcgccac agtggcagtg ctggatacgg tcagaatggt
     1261 gcgaaatgtt tgtgcacgtc cggacccttc ggcaacggct ggcttgtaac tttcggcagt
     1321 attgcccacc gcgattaaat cgccgtgctc atcaaacaca ccaatttccc ggatccagaa
     1381 tccgcccgtt tctggaggaa taaccagctc cgcaataatg cggttctgat gtgttgcgtc
     1441 caggatgacg cgattaacag tgtgtcgcca cacctcatgc accagacggg tctgcttact
     1501 gtctggtgtg ggcaacgcgc cgccaccgtc gcccacggcc atatgagtca ggcggacagg
     1561 cttaccatct ggcgcggctg cctgagctaa ttttttggca cctgtatcgg tgataacggt
     1621 tttaaatttt cgtgttgtgg tactcatgct taatcgtccg gataaatggt aataacttca
     1681 ccgtcatgag ttgccgccgc tgcaaaaata tcccccggaa tttcctgaat gatattcagc
     1741 cctgtcatgt ggcggctgac cggacgggca tcagcaatca accgctccat ttccagatac
     1801 atttcctccg tcacgccact gtccagtgtg ccgacttcaa cggtaaatgt tcccggttct
     1861 ccgccgaact cccaccactc agacacgcga atgaggtatc ccagcggctc aatggccctg
     1921 cgcagtgcgc tgatggtccc tttgtgtcgg tgtatcagcc atgcatcacg aatcacctgt
     1981 cgttttgtct cttccggcca gttgcgatcc cagcggtcaa cggaaaatgc ccaggcgaga
     2041 taaggcagca ggtgcaccgg gcaggtgtcc ggcgaccaca gcgtgttgag gtctaccggg
     2101 atgtctgtaa tgcgcgttcc gacagcttcg gcacaacgca tgaaactgct ggctgatggt
     2161 ggtaacagtg aattactcat tgcgcccacc ttcgctgatg gtgaacgact cacagcgcgc
     2221 cgcctgtatg tcgctgatgg ccatattctg tgtgggttcg attatctcca cgcgttgcac
     2281 accgtgcaca tgcagtgcgg cagcaatggc ggacaacgcc acgtcctgac cgataagccc
     2341 ctgctcagcc agccacttcc tgaacgacga ttcagccgcc gccagaatag gttcggattc
     2401 cgggccgggg taaaagtaca gttttgcatt cagccgccat gtcacgattc tggcgctctg
     2461 taccgtcagg cggtcggcca ccgggcgggt atcctctgca ttcagaacgg cgcgaacggt
     2521 attaagcaac gcctccgttg ctgtgccgtc gccttcagtg gacaggatgg aaaccgtcac
     2581 atttgccgga gacggactga tagcccgcgc atcacgcacc agaccgctgg cgctgcgggc
     2641 aaaatactcg tatgcacctg acgggccagc aacactcagg ccatcgtacg cccgctgcgc
     2701 ccgcagtctc agcgaggtgt cactttccat cactgcgtcg gtggtatccg ttgccggagt
     2761 gatggtcagg cgctttgtgt tcatattgcc cgcgaggttg tccaggtctg tcccggcgct
     2821 gtggcttaac atgcaggcgc gtgccccctc attgacccgc tggcgtaaca gcatttcacg
     2881 aaacgctgtt gtctgggcga taacgttcag gggttccgat tccagctcca gcgcggcgga
     2941 gacggcttca cgctgttcgg caggatagga cgcaatcatc atggcctttg tgtcagccag
     3001 aattgcctca aagtcaggct ccgcgatgat ggcgggttcc ggtaactggg aaaggtcaac
     3061 ggcaggcatg atttactccc tcagcgtgat ggttaattca acattctgca tggtctgcat
     3121 gacagtgccc gacagcgtca ccccggcgcg gcctcctgcc ttccagacaa cgtcgatggc
     3181 gtccagggca atgcggggtt cccatcgtgt cagcgcaatc acggcagcac tcatgcattg
     3241 cagacgagtg gtgttattca tgggttcgtc aattaaatcg ggcacaaggc tgccatattc
     3301 ccgtcgcata acccggcttg ccagcggggt ggtcaggatg tccctgactg actgtttcag
     3361 atgctccata tcgttcaggt ttcccgttcc gtccgggttc attcctgtgt agcgggttgt
     3421 cactgcgggc ctcctgtcga atcgctgcca cctttaacgc caccgtgttt atgcgtatgc
     3481 actgtgatgc cgtttgaggt gaaattgccg ccgctgtgcg tgatattgcc gctcatcttt
     3541 cccccttttg tgacgtcaag cgtcgccgtt ctcagaaggt ctgtgcattc cacgacgggc
     3601 gtgtccagtg tcacactgac ggatgcctgc agggtggccg ttttcatgcc gctggcgctc
     3661 agtgcgcctg cgtccgcgtc gtagcggaac accgcgccat ccggcgcgct gaccacgatt
     3721 tctttcaggc ttttgccggg ggccggattg gcatcactcc acaggctgcc aattatcatg
     3781 gcggtttccg ggttgccgcc aatgcaggca attaccacct gttcgccggg tgatggcggc
     3841 agccacacat tgaaggctcc cgcgcgcgtg gtgttccagc gcaaccagcc tgtttccagt
     3901 tcgccgctgc gaacgcgcac gcaccaggac ttctcatcaa cttcagagat gatcccagtg
     3961 cggatgatgt tgctcagcag tcgcatgagt tctgcgctca ccgtacagcc tccgcaatcc
     4021 ggcccagcac cgtgttataa atcaggcgtt catctgcctg actgatgccc aacagctcac
     4081 gtaccgggta atcggtgaaa atgcccggcg caacctgatc gcgctcaccg aactgatgaa
     4141 cgcgggcaat acgtgcggcc acgccgctgt aacccaccgt cacaccggaa gcatctgcac
     4201 gggctttcag gtagcgggcg gtgcgcagtt ttacgaacat ggggacgcgc tttgtgctgt
     4261 cctggttgat gcgccgggtg cgtatttcca gaaagcggtc gatgtcatcc cggtaaaacg
     4321 tgcggatatt gtttttatcc tcatcccacc cggtaatggt tcgcccgtat ttccccgtgt
     4381 cgtgatgcca gtttttcagc gtgcgtgctt cgttattcca gataaagcga atgcgctcct
     4441 gtatccgggt tacgcggcgt ctgcgtggtg tccacgcggt cccgtccggc gctttctgtg
     4501 accggatacg cgcctgctgg gcgcggcgta aatcctgtgc cagctttctg gcgatgttat
     4561 tgatggcctg ctgattcagg ctgtcgcgga tagcctcaaa ggtttcatcc acgcgggtga
     4621 atgccttatc catcgctttc accccacgtc acatcctgga atacatgcga ccagtcgcct
     4681 tcggaagatg gcagacgggg ttttggctcc tgcaggtgtt ctgcctgcgg tgccccctga
     4741 ctgctgcgcg tgatgcgaac gcgttcccgc agagggagcg taaacaggag atcggcgctg
     4801 tcatcgtcat tgataacggc ggagaatttg atgtcctgat tacgctccgg attaagcaac
     4861 aattgcggct gattttcgga taaccacgcc agtagcggca gcgtgaggtc gtccagctcc
     4921 ccggcgtaat ccatgacaaa catcaccatc tgatagcggt aaacaaacga gggcgtttct
     4981 ccggtcgttt caatgttgcc gctctccacg aaaatggtga atttttccgg gttggcctga
     5041 caccagcgac acccatgaat catgcccttc cggaggctgt ttgtcttaag catctgtttt
     5101 gctctcctgt tcttccttac gctgcaggac tatttatttc cagcaccaga ctttctatct
     5161 caacgccata cgttgcattt ttggtaatat ccgtcagcgt cagtgcattc agccccagtg
     5221 tcagactgtc ttttatgacc tggaatgccg ggccagccac tccattcagt ttcggagtaa
     5281 ccgtggcact gctggcggtg aacaccagct ccagcgtctg ccagtcgtta ctgtaattcc
     5341 cgaactcgcc caactttgtg tttcctgctt tcttgtgatg catcagattc agtttgccgt
     5401 ctgtggtctg ggtgaagaac gacatcagga acgggttacc agtcccggtc atcgccacga
     5461 cgtcaggtaa cgctacatcg gtatacagat aaattcccag
//
LOCUS       DAZGJW010000234         5440 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZGJW010000234 DAZGJW010000000
VERSION     DAZGJW010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.236"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..674)
                     /gene="ehxB"
                     /locus_tag="ACHKIK_004781"
                     /gene_synonym="hlyB"
     CDS             complement(<1..674)
                     /gene="ehxB"
                     /locus_tag="ACHKIK_004781"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFO9085791.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDV"
     gene            complement(724..3720)
                     /gene="ehxA"
                     /locus_tag="ACHKIK_004782"
     CDS             complement(724..3720)
                     /gene="ehxA"
                     /locus_tag="ACHKIK_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFO9085792.1"
                     /translation="MTVNKIKNIFNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGRTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGSLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSKLIQFVEKNNKHYQARMISELGDKDDVVYSGAGSSEVFAGEGHD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGREIKVDKIPHNNNERSGYIKASNIASEKNMVNITSVANDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            complement(3722..4237)
                     /gene="ehxC"
                     /locus_tag="ACHKIK_004783"
                     /gene_synonym="hlyC"
     CDS             complement(3722..4237)
                     /gene="ehxC"
                     /locus_tag="ACHKIK_004783"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFO9085793.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            complement(4760..5412)
                     /locus_tag="ACHKIK_004784"
                     /pseudo
     CDS             complement(4760..5412)
                     /locus_tag="ACHKIK_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(5015..5131)
                     /locus_tag="ACHKIK_004784"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         1591 a         1270 c          886 g         1693 t
ORIGIN      
        1 acgtcaatac gacttgtagt atgagagaaa atataagttc tggctccggt taatatcact
       61 tcaaaaagta tcactataat aaatgctatt gtgataatat ttaacgttga aaacccccgg
      121 tgaactaaaa ccttatccat tacaacctga aaaaaaagag gtgttattaa cgcaagaaac
      181 tgaataaaag cagaaacagt taacacctca agtaaaatcc tcctgtattt cacaacagag
      241 gggataaacc aagaaaaatc aaatttagct aactctccga ctactgttgc tcttgacgta
      301 accagaatga cttttccctg atacagtttt tcaaactcat cccgactaaa agttagtgac
      361 tgatgttgtt ctggatcata aacaagatag cgtgatgaat ctttagtaac acgagacaat
      421 atataatgct taccgtcatc ccgccatatc aacgcaggta aagaaattat tgacaatctg
      481 gaaaaatgtt tttctacata ttttgctttt aagccaatgg atttcgctgc cagtaaccat
      541 tcagtcaccc caaaatcttg tgtgtgggta ttatactgat gccttatagt ttcagcattg
      601 acagttatat tatgatattg tgcaagcaat atcagtgcat acagactatt atgagaacta
      661 catttactca tcatataacg atgaccattc ctcctggaat ggccatcacc tcctctttta
      721 gtctcagaca gttgtcgtta aagttgttga gtgtgtgttg ttttgatgta aggataaatt
      781 atataaagat gctaatcgtt catcacctga atcgaaccct gaaactgaag aaataatctt
      841 attaatatca ttggcaacac tggtgatatt aaccatgttt ttttcagatg ctatattact
      901 ggcttttata taacctgacc gttcattatt attatgaggt attttatcaa cttttatctc
      961 tcgaccatct ttatctgtta taacctcaac aagatgatta tctgctcctg aggcatcttt
     1021 cgcaaaccag tttttaaatg ttatcccatt gacatcattt gactcattaa atgaaagtac
     1081 accattaatg gctttattca tgataagatc atttccaact ctcttaaatg cgatatcatc
     1141 aaagctaata tcagataagt gcaaacgatc tcctttacct ccttcatctg caattgtatg
     1201 atgaccatag ttttgcctat aaacataaat atcattacca aaaccaccat tcagatagtc
     1261 attaccttcc cctccatcca gaagatcaat accaccacca ccataaagtt tatcatcacc
     1321 agtaccacct gacagaatat tataagctcc gtgtgcctga agctcatcat taccgcttcc
     1381 tcctgtcaga taattattac catcccctcc actcaattta tcgtttccac taccaccaaa
     1441 taactggtcg tctccctgtc ctccggatat ataatcatcc ccatcatcgc cgtatagtcg
     1501 atcattacca taattacctt cgatataatc gttcccatct gcgccatgga atatatcatt
     1561 aaacttaccg cctttgaatt catcatcatg ttttccgcca atgagctctt caacagaatg
     1621 aagattatct attacatcat aaggaattcc accggttctg aattcaaaat cacgatattg
     1681 tattttctct gttcgcttcc ctactgacac ctcctgttcc ttaacgactt cctgcaatac
     1741 cttcacgtca ccatacatat tttttgaaac tatatactca ccaggttttg atgctcctgt
     1801 tgcatcaatt gttagtttac caacatccgt cttattataa gatacggtat catgaccttc
     1861 accagcaaat acttctgatg agcctgctcc agaataaacc acatcgtctt tatctccgag
     1921 ttcagaaatc attctcgcct gatagtgttt gttattcttt tcaacaaact gaatcaattt
     1981 tgaataatca tatacacctt tatgattttt tatgcctttt acagaccatg tatccttacc
     2041 atttacaata agagatgtca tatattcata tttaccactc tgttttcttt ctctaacttc
     2101 ttctcctggg gtaaatgttg gtgttataaa tgtcaaaaca cttgatacat tacctgttga
     2161 aaggtctatg gtcccttttt gaggatcaaa cacttgttgt gtaaactctt tcggttgagc
     2221 ctctaaaagc cctccatttt ccagataatt aatatatgcc ttaccactct gactgcgatc
     2281 agcattacgg gttatgcctg caagttcacc gatcttctca tcccaatgtt gctgggttat
     2341 tgcgactgct ctttctactg catgctgacg agaaaaatca gcaagcaaag acagagagtc
     2401 ttctaaaaac gcagcatgtc ttgcgtcata gccattctca aaataatttt tgccatgctc
     2461 cttttcccat tcattgatcc gagcagcgaa tttctctgca acgtgctcaa acatagcctg
     2521 ttttgatgct tccagaatgc cagatatcgt accggttaat gcactcacca gcatgcttat
     2581 cggggcccct atgagggatg cactggaggc tgcactaact cccgcagata cagaactcag
     2641 gacagtattt attgttgtca gggctgcatc tatagctccg gtttctttat gaaaggctgc
     2701 gagtaaagca tccccttcat aattcaattt tttaaatcgt tcagaatatg attcaagctg
     2761 cttagctcgc tcaaatttat ctgcagcagc caggaaagaa agaggactga tagccagcat
     2821 aacagccgat gtgatcagac ccgcacttgc agctgttgtc gataacccct gtgccattct
     2881 ctgagccaga atatattgcg aaacagcttt accaacattt ccaagaacct gagttgtcag
     2941 ttcgataccc gctgcagctt ttgttcctgt atgtgcgtca ctgtttccca gaataaagct
     3001 tgctgataca gcagaaagaa ttccggagac aacatccagc ccatccccca gggagcccag
     3061 gtctggtaaa ttttgtaatt tcccaccaac agaacttaag cgaggtttac tggataaaaa
     3121 tgagccaagc tggttaagct gctcagaaaa tgaatcaacg gtactgttta tacttgatac
     3181 tgtatctaca agctgattaa taagttcaat actacttttg gcaatatcat tctgacttat
     3241 atcctctcct tcccgttgtt ttctcagcaa ttcatcaaga gccatgcctg ataaagcaat
     3301 ccccgtaaaa ttctgtagtg ctgagagaac tgttccggct tttcccagat tattacctac
     3361 attttcagcg gttcttccta ttttactccc aactttctga tacttctgca gaagtttgtc
     3421 aagttgtggt gcgaatattg caacaccacg ttcagttaat cctacaactt tttctgctgc
     3481 accaaaaaac tggtttgcaa tcgctgtatc atctcgttca gtacggtgta tttctattcc
     3541 aagctcatca gcagctttga ccaactcatt aatacccacg ccctgagctt cataattatc
     3601 aggtattaat aatatgagtt ttttgccagc ggaacgtacg cttgatgatg ctgtattaaa
     3661 tgctgatttt gtagtcaatg tcgcattatt gaaaatgttc tttattttat ttactgtcat
     3721 attagttttc tttctcaata ttaaaattaa aatcttgtct gttttttact tcagacataa
     3781 gttcatgatg gtattgcctg aatatttttg atgccagttt tttatctatc ccccctccat
     3841 gaaattcagc tatttttcct gttttactgc tctcatctaa ccttattgct ctgaataact
     3901 cataaggata ctttttcctc atgtatttat ataactccat gttgtgtcca aatggagcta
     3961 tccagtcaat aaaccacttt ctttctcccg acatccagtc ttttgcatac aaagatgtta
     4021 catcatttat atattttacc tcacattcca gatctaaact ggcccagcta caaaaagcta
     4081 caggaagctc atcctttatt aagagagcaa attgatttgt ctgaatagca ggtatgacat
     4141 taatagcaaa aacagataat ggccattttt tatgcagagg agaacaggcc catagccatg
     4201 caaccttccc catgacgtca aaagcattag atttcataat gtttaaataa ataagaaaac
     4261 tcagtaaatg taaagaatcc tatataaaag aacagaaatt atatcttttt aagcaagaag
     4321 catctaaaaa caaaaagcta ccgcaaggag aatctctccc aaccaaaaca acattagcga
     4381 taataatata ttgcctaaac attcttacac atatcacagc taagttatat tataacactc
     4441 agatgattga tttgtaagat aacgtatgat tttcatatga atagccacgt gacaaatgcc
     4501 ttattaaaga gattgtaaac agaatacagc aaactatttc agttaccgaa atgacgagtt
     4561 aatcagtttt gtttaccctg caaacacaaa atctaaacct acacagaaaa gcaaacctct
     4621 cctgttaata ataaatatct tccggtatag ctggagtttt ttcaaatgcg cctataaggc
     4681 tctcttacca gccgagccat aacaggcgca cacgatctga catttgcatc caacttcgtt
     4741 acttacggcc cgtaaatcgg ctgacggtag tacggacctt tttaccccgg agaacaccgg
     4801 caagtcccat aaccgccatg aggcgcgcca ctgtacatct ggccaccctg ataccttcgc
     4861 gtaacaactg gcgccagact ttacgcacac cgtatacctg atgattttca tcgtatacgc
     4921 gctgtatctc tctcttcagc cagtcatcgc gctgcgcacg ggcactgcgt ttatcaggat
     4981 gatgtcgctg ttgctgacag tggtaatacg ttgacggggc aatatgcagt tcactgcata
     5041 ccggtccgac cccgtactgc tcacgcagct tatccagcag aggcattatt ttttccagag
     5101 gcggtcgaac tccgccttcg caaaataagc ggaagcctgg cgaaggatat cgttactgcg
     5161 gcgcagttca cgattttcac gttccagctc tttcagacgc tgacgttcag cggtggtgag
     5221 tccaccatca ccactcccgg tatcccgctc atgctgacga acccacacac gcagagtctc
     5281 tggtgtacag ccaatctttg gggcaatgga acaaattgcc gcccattgtg agtcatattc
     5341 gccctgactt tccagaacca tacgaactgc ccgttgacgg acctcggggg aaaaacgtgt
     5401 atttttagtc atcctgttta cctctttctc agggagttta
//
LOCUS       DAZGJW010000235         5425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZGJW010000235 DAZGJW010000000
VERSION     DAZGJW010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.237"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(118..366)
                     /locus_tag="ACHKIK_004785"
     CDS             complement(118..366)
                     /locus_tag="ACHKIK_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO9085794.1"
                     /translation="MGAYLGSKVKGEDPTVPMVGNAIGTVLGNKAGEKFTKEMLSKGY
                     SPVTSDVIGTVSGGTMTIGTAIDFGIEESQKKGDSNEK"
     gene            730..924
                     /locus_tag="ACHKIK_004786"
     CDS             730..924
                     /locus_tag="ACHKIK_004786"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-zinc-cadmium resistance protein"
                     /protein_id="HFO9085795.1"
                     /translation="MVFVATQHGQKVEFTPQPVKLGQSSGDSQWIEVVNGLNQGQKYA
                     AQGSFLLKSEMEKGEASHEH"
     gene            914..1750
                     /locus_tag="ACHKIK_004787"
     CDS             914..1750
                     /locus_tag="ACHKIK_004787"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFO9085796.1"
                     /translation="MSTKSPHIPDHESVKPEGLFDRLIQFSIHNAIWVMLFVAAWIAI
                     GIYSYQKLPIDAVPDITNTQVQINTSANGFTALEMEQRITYPIENAMSGMPKMEQTRS
                     ISRYGLSQVTIIFEDGTDIYWARQLINQRLQEAMGEMPEDVQPNMSPISTGLGEIYQW
                     VIKAEPNAKKPDGTPYSAMDLREIQDWIVRPQLQRVKGVAEINSIGGFNKTYVVAPDL
                     NRLQQLQIPLSDLQTALTENNENRGAGYIEKNGQQLTVRVPHSIVNKTFLSFIFNGLF
                     SC"
     gene            1991..2806
                     /gene="sul2"
                     /locus_tag="ACHKIK_004788"
     CDS             1991..2806
                     /gene="sul2"
                     /locus_tag="ACHKIK_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFO9085797.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2893..3195
                     /locus_tag="ACHKIK_004789"
     CDS             2893..3195
                     /locus_tag="ACHKIK_004789"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085798.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(3579..4415)
                     /locus_tag="ACHKIK_004790"
     CDS             complement(3579..4415)
                     /locus_tag="ACHKIK_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFO9085799.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(4415..5218)
                     /locus_tag="ACHKIK_004791"
                     /note="aph(3'')-Ib"
     CDS             complement(4415..5218)
                     /locus_tag="ACHKIK_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFO9085800.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1312 a         1460 c         1356 g         1297 t
ORIGIN      
        1 ctaccggctt taaagaaact gaccacttgt ccaaaaagaa tcccagaaaa gctaagtata
       61 gatattagca atatacacct tgaaaaaaga tagaataaca gccataagtc aggagattta
      121 tttttcatta ctatctcctt tcttctgaga ttcttcaatg ccgaagtcga tagctgtacc
      181 tatagtcata gtccccccag agacagttcc aataacatca gaagttactg gcgagtatcc
      241 cttcgatagc atttctttgg tgaatttctc tccggctttg ttacccagaa cagtacctat
      301 tgcattcccc accattggca ctgtagggtc ttcgccttta actttacttc ctaagtaagc
      361 tcccattgtg ttaataaatg tgctgaaaat gacactttta ccctgtgtaa gcccaccttc
      421 acctgcggca ataagagtat ccgtagcact atatttctca cccggcttaa gggttaaata
      481 ctgcttaatg ccatctgtta cacctcctaa aaggctaccc ccaataacag caacggtacc
      541 cgcggctggg actataagtt cgccaccaag aacagaagca cctgctcccc atgcaatcag
      601 taaacctctg acaggatcct gtccttccgg aagattacct gttgcaagtt tatcagaagc
      661 atacttaatt gtgaaagtac cgcattacgg gtacttaagt ccgcagttca gaatgtcgat
      721 ggaaaagatg tggtttttgt cgccactcag catggacaaa aagttgaatt cactccgcag
      781 ccggtgaaat taggtcaatc gtctggggat agccagtgga ttgaagtggt aaatgggctt
      841 aatcagggac aaaaatatgc cgctcaaggc agtttcttac tgaaatctga gatggaaaaa
      901 ggagaggctt cacatgagca ctaaaagccc tcacattcca gaccacgaat cggtaaaacc
      961 tgaagggctg tttgatcggc tgatccagtt ttctattcat aacgcaatct gggtcatgct
     1021 gtttgttgca gcctggattg ctattggtat ttatagttat caaaagttac cgattgatgc
     1081 tgtaccggat attaccaata ctcaggtgca gattaacact tcagccaatg gctttacggc
     1141 tttggaaatg gaacagcgga ttacctatcc aattgagaat gccatgtcag gcatgccgaa
     1201 aatggaacaa acccgttcga tttcacgcta cggcttatcg caagtgacca tcatttttga
     1261 agatggcacg gatatttact gggccagaca gcttatcaac cagcgcttgc aagaagcaat
     1321 gggagaaatg cctgaagatg tgcaacctaa catgtcgcct atttcgacag gtttaggtga
     1381 aatctatcaa tgggtgatta aagccgaacc aaatgctaaa aagccggatg gaacccctta
     1441 ttcggcgatg gatttacgtg aaattcagga ttggattgta agaccgcaat tacagcgcgt
     1501 aaaaggcgtt gcggagatca acagtatcgg tggctttaat aaaacctatg tggttgcccc
     1561 tgatctgaac cgattgcaac aattgcaaat tcctttgtct gatttacaaa ctgccttaac
     1621 agagaataat gaaaaccgtg gtgctggcta tattgagaaa aatggacaac agctaacggt
     1681 tcgtgttccc cactctattg taaacaagac atttttatct tttatattca atggcttatt
     1741 ttcctgctaa ttggtaatac catgaaaaat accatgctca gaaaaggctt aacaatattt
     1801 tgaaaaattg cctactgagc gctgccgcac agctccatag gccgctttcc tggctttgct
     1861 tccagatgta tgctattctg ctcctgcagc taatggatca ccgcaaacag gttactcgcc
     1921 tggggattcc ctttcgaccc gatcatccgt atgagactca tgctcgatta ttattattat
     1981 agaagccccc atgaataaat cgctcatcat tttcggcatc gtcaacataa cctcggacag
     2041 tttctccgat ggaggccggt atctggcgcc agacgcagcc attgcgcagg cgcgtaagct
     2101 gatggccgag ggggcagatg tgatcgacct cggtccggca tccagcaatc ccgacgccgc
     2161 gcctgtttcg tccgacacag aaatcgcgcg tatcgcgccg gtgctggacg cgctcaaggc
     2221 agatggcatt cccgtctcgc tcgacagtta tcaacccgcg acgcaagcct atgccttgtc
     2281 gcgtggtgtg gcctatctca atgatattcg cggttttcca gacgctgcgt tctatccgca
     2341 attggcgaaa tcatctgcca aactcgtcgt tatgcattcg gtgcaagacg ggcaggcaga
     2401 tcggcgcgag gcacccgctg gcgacatcat ggatcacatt gcggcgttct ttgacgcgcg
     2461 catcgcggcg ctgacgggtg ccggtatcaa acgcaaccgc cttgtccttg atcccggcat
     2521 ggggtttttt ctgggggctg ctcccgaaac ctcgctctcg gtgctggcgc ggttcgatga
     2581 attgcggctg cgcttcgatt tgccggtgct tctgtctgtt tcgcgcaaat cctttctgcg
     2641 cgcgctcaca ggccgtggtc cgggggatgt cggggccgcg acactcgctg cagagcttgc
     2701 cgccgccgca ggtggagctg acttcatccg cacacacgag ccgcgcccct tgcgcgacgg
     2761 gctggcggta ttggcggcgc tgaaagaaac cgcaagaatt cgttaactgc acattcggga
     2821 tatttctcta tattcgcggt tcagcaggca tgtccccttt gagggcgacc cgacgacagg
     2881 ataatcgacc ttatggtgcg caaatatttc ggcacagacg gtattcgtgg caaagccaac
     2941 gaaggcgcga tgacggcgga aaccgccttg cgcgtcggca tggcggctgg ccgtgtcttt
     3001 cgtcgcggtg accaccgcca tcgtgtcgtg atcggcaagg atacgcgcct gtcgggctat
     3061 atgcttgaac ccgcgctcac agccggtttc acctcgatgg gcatggacgt attccttttt
     3121 ggcccgctgc cgacaacgta taggaagaat aaacgccctt ttcacccaag tccaacagct
     3181 ttggaccgca gttgactctt tcgacacccc tgcgatgcaa cccaatccgg ctgacgggga
     3241 gccagcaacg ctgaaaattt accctcctct ttcccactag cggctccttt tccgacaacc
     3301 agcacggcgg atccctgccg cggcgctgtg aacgcagcat tttgattggt atcgttggcc
     3361 ttcaggctcg tcagtcaaac agacccagga gcagctcagc cggtggcgcc cggctttcgg
     3421 gtaacgccct ggtcccgctg gtttcggctt tgtgcttcag gtgatcaagg atggggtcgt
     3481 ttgcgggaga gggcgaaatc ctacgctaag gctttggcca acgatattct ccggtaagat
     3541 tgatgtgttc ccaggggata ggagaagtcg cttgatatct agtatgacgt ctgtcgcacc
     3601 tgcttgatcg cggccgcgat agctagatcg cgttgctcct cttctccatc cgcgttccaa
     3661 gctgcggaaa ggcacccata agcgtacgcc tggtcgagca ggcgacgcgg atcgacgtcc
     3721 agcgcacgag agaatgcgtc cgccatctgt gcaatgcgtc taggatcgag acaaaggtcg
     3781 tctctgtcag ccggatcgta gaacatattg gcggcgccaa agcccacttc accgaccaga
     3841 ccgacgggat ctatcaccag ccagccgcga ctggagaaca tgatgttttc atgatgcaga
     3901 tcgccatgta gcccacgcag ttccgaggca ttgctcatca tttgatcggc tataatcgcc
     3961 gcgtggacgt agtcagtttg acaacctgcg ttttgatcat cgcgcgcccg ctgaaacaaa
     4021 gctgcaaagc gatcccggat cgggagaagg gcagaaggca ggggttcctc agatgcggca
     4081 tacagcttcg ccattagttc cgctgcaatt tcggtcgcct ggtagtcgcc gtgctcggca
     4141 acgatgtgag agagcattcg ctccccggca tattcgagca acatcagatt gttctcacga
     4201 ccgagcaacc ggactgctcc cctcccattg cgccatacca gatagtcggc cccgcgcagt
     4261 tcatcagcaa tgtcttctat aggtttcaat cccttgacga ttgcaggagt cccgtctggc
     4321 aatgaaactt tccaaacgag gctggaaaag gtgtccgcaa tgagaacagg ttgcgaaacg
     4381 tgccaatgag caggaaaaac aggcggcatg aacatcaacc ccaagtcaga gggtccaatc
     4441 gcagatagaa ggcaaggcgt tcgcggtcgg gggcttcgat ccccaataca ttgaatagga
     4501 cagcgaaggc gcgctctgct tcatctggcg ctgcccagtt ctcttcggcg ttagcaatca
     4561 tgagtgccaa atcggcatag cgatctgctg ttccgagccg cccaaggtcg atcagacccg
     4621 tgcattgaag agttttaggg tccaccatga agttcggcat gcagggatca ccatggcaaa
     4681 caaccatatc ggtgcgctct tggtcgagcc gcaccggtag ctctcgttcg acacgagcca
     4741 aaagatcgag ctgcggcgta ctcttgtcct cgtccggtaa gaagtcggga ttgacggcat
     4801 tgcgggacac cacatcaacg gcgcgtccga acattcgcga cagcctgcgc tcaaacggac
     4861 attgatcaac cgataggctg tgaacagcgc caagttgctg ccccattgac ggccacgctt
     4921 tgagcaaatc cgctccagac agatcagccg ccggtactcc cggaattgcc gttatcacca
     4981 agcatgcacc ctcctgttcc tcctgccagt tgatcacctc ggggcaagcc acacctcgac
     5041 ctttgagcca aatgaggcgg tcacgctctc cagcgagctc accgcggcgg gaagcaggtg
     5101 cgattttcgc gaaggcatgc ccgtcaccac gtcgaaaaac aaaatcacca gattctccgc
     5161 ctctgacagg caaccagtca gaatgcgatt caccaaaaaa aatattagtt cgattcaatg
     5221 gaggttcctt cagttttctg atgaagcgcg gaggtggctc aacctgcgaa aagaaacgag
     5281 ttgctacgta agtccgagaa catgctttcc atggtcttgc accggttgtg ttccggtcta
     5341 tctctcattt taagcggctt ttttctcaaa tgccaccggc gatttccagc cgagtgctga
     5401 tgaatcccct aatgattttt atcaa
//
LOCUS       DAZGJW010000236         5414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZGJW010000236 DAZGJW010000000
VERSION     DAZGJW010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.238"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            43..489
                     /gene="hpaR"
                     /locus_tag="ACHKIK_004792"
     CDS             43..489
                     /gene="hpaR"
                     /locus_tag="ACHKIK_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFO9085801.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            608..2272
                     /gene="tsr"
                     /locus_tag="ACHKIK_004793"
     CDS             608..2272
                     /gene="tsr"
                     /locus_tag="ACHKIK_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO9085802.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMAVADSGENWETF"
     gene            complement(2315..3586)
                     /locus_tag="ACHKIK_004794"
     CDS             complement(2315..3586)
                     /locus_tag="ACHKIK_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO9085803.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEVVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAAAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLTLVTYIPAISLFLV"
     gene            complement(3583..4056)
                     /locus_tag="ACHKIK_004795"
     CDS             complement(3583..4056)
                     /locus_tag="ACHKIK_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO9085804.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVMTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAVLVAVLSIALLLYMAWLG
                     YRLAEMAQFKVTQLLKVSWFWIDLAVPVGALATAIYTLYWLIHDIKQSDSGDKMQ"
     gene            complement(4120..5091)
                     /locus_tag="ACHKIK_004796"
     CDS             complement(4120..5091)
                     /locus_tag="ACHKIK_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HFO9085805.1"
                     /translation="MSKIIAMLITVSTLFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFSTLSPEQLEMIHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
BASE COUNT         1425 a         1279 c         1378 g         1332 t
ORIGIN      
        1 tgtttctata ttaatgattt tgaatttttc aggggaaaga taatgcacga ctctctaacc
       61 attgcgttgc tccaggcgcg agaagcggcg atgagttatt ttcgcccaat cgttaaacgg
      121 cataatttga ctgagcaaca gtggcgcatc gtgcgtattc tggcggaaag cccatcaatg
      181 gattttcacg atctggcgta tcgcgcctgc attttgcgcc caagcctgac cgggatcctc
      241 acgcggatgg aacgcgacgg tttagtgttg cgattaaagc cgatcaatga tcagcggaag
      301 ctctatatct cgctgaccaa agaggggcag gcgctgtata accgcgctca gacacagatt
      361 gaagaagcct atcggcagat tgaagctcag tttactgccg agaaaatgca acagttaacc
      421 catctgttag aggaatttat tgctctgggt aattcccgac aagaagacat cccgggcgat
      481 aatgaataac tttttagtat ctgataacca gcgcctggtt tttccatgga tggcgggtta
      541 caccttttca taaagttttt gctttccagg ccgaaaatct tgcatcggtc cacaggaaag
      601 agaaaccatg ttaaaacgta tcaaaattgt gaccagctta ctgctggttt tggccgtttt
      661 tggcctttta caactgacat caggcggtct gttctttaat gccttaaaga atgacaaaga
      721 aaatttcact gttttacaaa ccattcgcca gcagcaatcc acgctgaatg gcagctgggt
      781 cgcgttgttg cagacgcgta acaccctcaa ccgcgcgggt atccgctaca tgatggatca
      841 gaataatatt ggtagcggtt caaccgttgc tgagctgatg cagagtgcca gtatttcgct
      901 gaaacaggcg gaaaaaaact gggcggatta cgaagcgttg ccgcgtgacc cgcgtcagag
      961 caccgccgca gcggcagaga tcaaacgtaa ttacgatatt tatcacaatg cgctggcgga
     1021 gctgatccaa ctattaggtg caggcaaaat caacgagttc tttgatcagc cgacccaggg
     1081 atatcaggac ggtttcgaga agcagtatgt ggcttacatg gagcaaaacg atcggctcta
     1141 tgatatcgcc gtcagcgata acaatgcctc ctacagccag gcgatgtgga ttctggtggg
     1201 cgtgatgatc gtcgtactgg cggtcatctt cgccgtctgg ttcggtatta aagcctcgct
     1261 ggtagcgcca atgaatcgcc tgattgacag cattcgtcat attgcaggcg gcgatctggt
     1321 gaaaccgatt gaggtggatg gctctaatga gatggggcaa ctggcagaga gtttgcgcca
     1381 tatgcaggga gagctgatgc gtaccgtcgg tgatgtgcgc aacggggcca atgccatcta
     1441 tagcggtgcc agcgaaatcg ctaccggcaa taacgatctc tcttcgcgca ccgagcaaca
     1501 ggccgcttcg ctggaagaga cggcagccag catggagcaa ctgaccgcaa cggtgaaaca
     1561 gaacgccgag aatgcgcgcc aggccagcca tctggcatta agtgcttctg aaacggcgca
     1621 acgcggcggc aaagtggtag ataacgtggt gcagactatg cgcgatatct ccaccagttc
     1681 gcagaaaatc gccgatatta tcagcgtaat tgacggcatt gccttccaga ccaatattct
     1741 ggctttgaac gcggcggttg aggctgcgcg tgcgggtgag caaggacgcg gttttgcggt
     1801 ggtcgcggga gaagtgcgta atctggccca gcgtagcgct caggcggctc gtgaaattaa
     1861 aagcctgatt gaagactcgg tgggaaaagt ggatgttggc tctacgctgg tcgaaagcgc
     1921 cggggaaaca atggcggaga ttgtcagtgc tgtgacccgc gtgacggaca ttatgggcga
     1981 aattgcttct gcttctgatg agcagagccg tggtatcgat caggttggct tagcggttgc
     2041 tgagatggac cgggtaactc aacagaacgc cgcgctggtg gaagaatctg ccgccgccgc
     2101 cgccgcgctg gaagagcagg ccagtcgcct gaccgaagct gtggcagtgt tccggattca
     2161 gcaacagcag caacagcagc gtgaaacatc ggctgtggta aaaaccgtga cgccagctgc
     2221 gccgcgtaaa atggccgtgg cagatagcgg ggagaactgg gaaacgtttt aacgcaatga
     2281 cgccggatga gtatttcatc cggcgttttt attattaaac caggaacagt gaaatcgccg
     2341 ggatatatgt caccagcgtt agcattaata gtagagccgc cagtagaggc agggattgtt
     2401 taaagaaatt tcccaccgaa acttgcgtga tgttacatac cagcagcatc acggtaccta
     2461 ccggtggcgt gaatgcgcca atcgacaggt tgaagatcat gatgatgcca aaatgcaccg
     2521 gatcgatgcc aaagtgttcg accaccggtt ttaatagtgg taccaggatc agcaaaatgg
     2581 cgtttccttc aagaaacatc cccagcgtca gcagcaatat gttgataatt aacaggaaca
     2641 tatatttatt ttcggtcagc gaagtcacaa aattcgccac tgcgacacca gcatgttcca
     2701 ttgagaaaat ccatcccaac gcggcgctgg ccatgattac cagcatcacc gatgcggttg
     2761 cgcgtgccgt ttcgcccaat gctttgacga catgagaaat ccgcatttca cgatagataa
     2821 aaaagccaat cgccagcacg attagcaccg ctgctgcacc tgcttctgtt ggggtgaaaa
     2881 tgttagcgcg aataccaccc accacagcaa aaatgaggaa cagcgcaggc caggcctgtc
     2941 ccagcgagaa caccacctct ttgcccgtag gccagctttc acgcgagggt ttagcgttag
     3001 ttttgcgggc gaccaggtag acggtaaaca tcagaacgac tgcgcatagc aaaccaggta
     3061 ccagcccagc gataaacatt tttccgatag agacgtcggc aaccagaccg taaataatca
     3121 gggctattcc cggcggtatg atcggggtga ctaacgagcc accagcggta atggcggcgg
     3181 caaacgcgcg gttgtagcct ttacgctcca tctccggcac catcatgcgc gacagcatcg
     3241 ccgcgtcagc aaggttagag gcgctgacgc cgcccatcat ggtgctgacg agaatgtttg
     3301 ccacgccaag tccgccgcgc attttcccca ccagaacgtt ggcaatgtgc aggatgcgtt
     3361 cggcaatgcc ggtgtagctc attagtgtgc cgagcaagat aaaaaacgga attgccagca
     3421 atgatgggct ttgcgttggc gcgatcagtc tttgcacggc aatttcaatc ggtacgctgg
     3481 agaagaataa aaagtaagcg atgatgccaa taaacatcgc cagatagacc cgaatgttga
     3541 gaaacaaaca gacgatcatt agcggaataa tcaataacca actcattgca ttttgtctcc
     3601 gctatcagat tgttttatat cgtgtattaa ccagtagagg gtataaatag ccgttgccag
     3661 tgcaccgaca gggaccgcca gatcgatcca gaaccaggaa accttcagaa gttgtgtcac
     3721 tttaaactgc gccatttcgg ctagtcggta gcccagccag gccatataca gcagcagtgc
     3781 gatggagaga acggcgacca gtactgcaat aacacgcctg agcttgcctg gaagcatctc
     3841 catcaggaag ggaatggtca ggtgcgccct gtcacgttcc gccaccactc cacctaacat
     3901 caccacccaa atcatcagca ttcccgacat ttcttctgtc cattgaagcg gttggccgat
     3961 gaaataacgc ataaataccg ctccaattgt catcacaacc agaacgaata gcgcaaaggc
     4021 agcactaaat tctgccactt tgccaaaaag acgcatgaaa cctcctgtta gtgcgtgtcc
     4081 tgcctaagag agatgaggca ggacgctgag cattaatgct tattgcagtt gctgttgaat
     4141 ggtttcgtac agacctggtg tccattccgg gaattgagag taaacttcgc gtgctttctt
     4201 ctggaatggt ccagtatcgg gatagatcac ttcgacgccc gctgcctgca tggttttcaa
     4261 catttcggca tcacgttcaa gagtgagttt ctggctatag acacccgctt catagccggt
     4321 ctggtggatc atctccagtt gttctggtga aagggtgcta aagaaggctt cacctcctat
     4381 ccatactgag gtatttgtca ggtagttaac cattgtcaga tatcttgcct gctcatacag
     4441 tttttgccct tgcagcactg aaattgggtt ttctacgccg tcaataacgc cctgggttaa
     4501 tgcgggataa acttcgccta atggcatggg agttggtgtt gcccccatcg cctgaatggc
     4561 ttttatttgc atgacgttat taggaacacg aatttttaag cctgcgagat cgtcaacagt
     4621 gcgaatgggt tttttcgaaa taatctgtcg agtgccatag agataattat tcattaccac
     4681 atgaattccc tttttcttca gactttcatt tttttgttta aaccagtcgc tttcatatat
     4741 tttgaacagt ttttgcggat cgtcagtcag atacggtcca aacaaaatac ccaaatcggg
     4801 ttcataatcg gcaagaaagg ccacatcact caaggttatg acattcatgc ccatcatggc
     4861 ttgctcggtg acatcctgtt ttgagcccaa ttgggaactt ggatagagcg caagtgttat
     4921 ttcgccatta ctttttttat ttaataggtc tgcccagtaa cgcatgacca catccagggg
     4981 ttctccaggg ttattttcat aggcaacttt tatcgaaata ggttttgcct ggattattga
     5041 cggtaaaaaa agggtactga cagtaattag catcgctatt attttgctca tctttactta
     5101 ctccatgcta aggaaataaa actccaacat tgttgtcggg agtgaaacct tatgaaaaag
     5161 cagtcattac tgaaaagtag tcagcgaata gcggaatggc tgcaaatgtg tcataaattc
     5221 gtgcaccggg taatactttg ctgcttttaa tcggttttta atgcattttt tgattgtata
     5281 tcttaaagtt atgtaatgta tgacgttaca aggaacaccc actgatgagt ttagttaccg
     5341 ctgataagat ctcttattcg gttgatgatt tattaaaata aaagcattaa tttggctgcg
     5401 gctattttta caga
//
LOCUS       DAZGJW010000237         5363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZGJW010000237 DAZGJW010000000
VERSION     DAZGJW010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.239"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(204..839)
                     /gene="msrQ"
                     /locus_tag="ACHKIK_004797"
     CDS             complement(204..839)
                     /gene="msrQ"
                     /locus_tag="ACHKIK_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO9085806.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSV"
     gene            complement(840..1844)
                     /gene="msrP"
                     /locus_tag="ACHKIK_004798"
     CDS             complement(840..1844)
                     /gene="msrP"
                     /locus_tag="ACHKIK_004798"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO9085807.1"
                     /translation="MKKNQFLKESDVTAESVFFMTRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNDLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            complement(1953..2366)
                     /gene="hiuH"
                     /locus_tag="ACHKIK_004799"
     CDS             complement(1953..2366)
                     /gene="hiuH"
                     /locus_tag="ACHKIK_004799"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO9085808.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2499..3170
                     /gene="hprR"
                     /locus_tag="ACHKIK_004800"
     CDS             2499..3170
                     /gene="hprR"
                     /locus_tag="ACHKIK_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO9085809.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3170..4528
                     /gene="hprS"
                     /locus_tag="ACHKIK_004801"
     CDS             3170..4528
                     /gene="hprS"
                     /locus_tag="ACHKIK_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO9085810.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPATETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLFLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSEGQGLGLSLVKAIAELHGGSATYHYFNKHNVFRITLPQ
                     RN"
     gene            complement(4636..>5363)
                     /gene="hchA"
                     /locus_tag="ACHKIK_004802"
     CDS             complement(4636..>5363)
                     /gene="hchA"
                     /locus_tag="ACHKIK_004802"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO9085811.1"
                     /translation="KPYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLH
                     AAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKIADVVVSLNADS
                     EYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLN
                     GYSICAFPDAADKQTPEIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTG
                     DTPFAANALGKLAAQEMLAAYAG"
BASE COUNT         1487 a         1161 c         1262 g         1453 t
ORIGIN      
        1 ataacaaaat gcaacaactc ttaggagaca agagaatagt tctctcaggt gtgcttatct
       61 atcttattga aataaaataa attatgtcga tgatttgagg ttgatgacaa acgaaacata
      121 acttgatgtg atctgcgtag gccggatagg gcgctcgcgc cgcatccggc agggataacg
      181 cgtactttgt cggcaatatg aagttataca gataatttgt tgtgaacctg cttgcgtaac
      241 cggttaaaaa gggaaagcaa cttcttatac cgtaaggcta aaagcagtac cgccagccca
      301 gcgtagatga gtggctgcgg tgaaataatc ttcactgacc acaaataatg tattggggcc
      361 aggatcgcga caagatagac gaagttatgc aactgttgcc agtgtttacc gagttttcgc
      421 tgcatcgcct gggttgaagt gaacgctaac gcaagcaaaa ttacccagct gataatacct
      481 aacgttaaat aaggccgggt aattaactcc ttacctaata acgccagatt attcacgccc
      541 agctccagca atgcgtaact ggttaaatgc agtgtcgccc aggcaaagca ccataagcct
      601 aacaggcggc gagtgcgtat caataacggc tgttttgcgt agcgtgccag aggtgtgatt
      661 aacaaggtcg ccagcaaaaa tttcagtgca gtgcgaccag taaaatgctg aatatctttc
      721 accggatcgg cacccaatcc accgtgatta atcgcccaga ccagccagag aaacggcaac
      781 aatccggcaa gatgcaggca aactttcagc catgtcacct gttttgctgt cagacgcatt
      841 tagaaattct cccgcaaatc caggccacga tacagcgatg ccacctgctc ggcgtaacca
      901 ttaaacagta gcgttggctg gcgctgtaca tcgaggatgc cgcctgaacc aataaatcgt
      961 tcggtagcct gtgaccagcg cgggtgatca acatgcggat taacgttggc gtaaaaaccg
     1021 tattcgtcag gcgctgccag attccaggtg gttggcggac gctcgcgggt cagcttaata
     1081 ctgactatcg atttaatccc tttaaagcca tatttccacg gcacaatcag tcgcaccggc
     1141 gcgccatttt gtggcggtaa cgccttgcca taaacaccca cggtcatcag tgtgagcgga
     1201 tgcattgctt cgtcgagacg caatccttcg acataaggat atttcagccc gccaccgata
     1261 aagcggtcct gctggcctgg catttgttcc ggtgcataaa ttgtttcgaa agcgacatac
     1321 ttcgcattgc tggtgggttc ggcaagcgcc agcaatttgt gcagcggaaa accaatccac
     1381 ggcaccacca tcgaccatgc ttccacgcag cgcatacgat aaatacgctc ttccagcgga
     1441 aagcgacggg ttaaatcatc gtgatcgagg gtcaatggtt ttgccacttc gccgctgatt
     1501 ttcagtgtcc atggatcggt tttcaggcta ccagcattag cggcgggatc ggctttatcc
     1561 agcccgaatt catagaagtt gttataaccg gagactttat ctgctggcgt cagtggcagg
     1621 tcattttgcc aggcggcagg cttgctgaac tccaacggtt ttccggcggg ggcgggtggg
     1681 cgatcattcc ctttgaacca gctaagcaga tcggcatgcg cagcgtgagg caaagaaagt
     1741 gcagccgcgc tgatgcccag tgcttttaac acctgccgac gcgtcataaa gaataccgac
     1801 tcggccgtaa catctgattc ttttaaaaat tgattctttt tcataagcca tcacactttt
     1861 ttgtctttag ccagcaagag ccgtttactg ttaatctgtc aggaagtgtc ctccctaaca
     1921 gtagcagaga tctgctttta catatttatg cattaactgc cacgataggt tgaataccca
     1981 tattggctaa gtaataaagg cacatgataa tgctcgttca ctttattaat atgaaattca
     2041 acggggatct cagggaagaa actttcaaga ttttgtttct tgaaatagtc cccggtttta
     2101 aatacgacac ggtaatcgcc cgtagttgca gtttgctcgg gccacagtgc cttaattcgt
     2161 ccatccttat ctgttttggc ggtattaagt tgtaaccagc cgttgtccgc tttcttttca
     2221 agagtgactg tcacgtcggc agcgggtttt cctgtttgct ggttcaaaat gtgcacacta
     2281 agaatgtttt gttgtgcagc atagaccaaa gaaggtaatg aaaatgcagc cgttactacg
     2341 gagagtacta aataacgctt taacatgttt atatccttgt catgtgaatg agttttgagg
     2401 atagcgaaaa agcgtccacg gtaaccttac agcagcatta caattttgta atgaaccagg
     2461 ctgtttctat aacatatgat ttatggcata ttattttcat gaagatttta cttattgaag
     2521 ataatcaaag gacccaggag tgggtaacgc aggggctttc cgaagcgggt tatgtcattg
     2581 atgccgtttc tgatggcaga gatgggcttt atcttgcgct taaggatgat tatgcattga
     2641 tcattctgga tattatgctt ccgggtatgg atggctggca gatcttacaa acgttaagaa
     2701 cagcaaagca aactcctgtt atttgcctta ctgcaagaga ttctgtcgat gacagagtca
     2761 gagggctgga cagtggggca aatgattatc tggtaaaacc tttttcattt tctgagttgc
     2821 tggcaagggt tcgggcacaa ttaaggcaac atcacgcttt gaattcaaca ttagaaatca
     2881 gtggcttaag aatggactct gttagtcaaa gtgtgagcag ggacaatatc agtattacac
     2941 tgacgcgcaa ggagtttcag ttactttggc tactggcctc cagagctggc gagattatcc
     3001 ccagaacggt tattgcgagt gaaatttggg gaatcaactt tgatagtgat accaatacgg
     3061 tggacgtcgc cattcgcagg ctccgcgcaa aagttgatga tccttttcct gaaaagctaa
     3121 tcgccacaat ccgggggatg ggctattcat tcgtagcggt aaaaaaataa tgaaaagatt
     3181 atctataacc gtccgtttaa ccttgctttt tatattgctg ctatctgttg ctggcgctgg
     3241 aattgtatgg actctctata atggcctggc aagtgagttg aaatggcgcg atgatacaac
     3301 actcattaac cggacagcgc agatcaagca gttgttaatt gatggggtaa atccagatac
     3361 gttacctgtg tactttaacc ggatgatgga tgttagtcag gatatcttga tcattcatgg
     3421 tgatagcatc aacaaaattg ttaaccggac gaatgtcagt gatgacatgt taaataacat
     3481 acctgctact gagacaatca gcgcagctgg catttacaga agcattatta atgatacaga
     3541 gatagatgct ttacgaatta atattgatga agtttcgcca tcattaacgg ttactgtggc
     3601 taaattggct tcagccagac ataacatgct tgaacagtat aaaattaata gcattataat
     3661 ttgcattgtc gccattgtac tttgctcagt attaagtccg ctgttaatca gaacgggatt
     3721 acgagagatc aaaaagttga gtggtgtaac ggaagcgctg aattataacg atagccggga
     3781 gcctgttgag gttagcgcat taccgagaga actaaaacct cttgggcagg cgttgaataa
     3841 aatgcatcat gctttagtca aagattttga gcgtctaagt cagtttgctg acgatctcgc
     3901 tcatgaactt agaacgccca ttaatgcatt actgggtcag aatcaggtta cgctcagtca
     3961 aaccagaagt atcgctgaat atcaaaaaac aattgccggt aacattgaag agctggaaaa
     4021 tatttcgcgg ttaacagaga acatactgtt tcttgcccgg gcagataaaa acaatgtttt
     4081 ggtgaaactg gactcacttt ttctcaataa ggaagtcgaa aatttgttgg attatcttga
     4141 atacctttca gacgagaaag agatttgctt taaggtcgag tgcaatcagc aaatctttgc
     4201 ggataaaatt ttactgcaac gaatgttatc gaatcttatt gttaatgcca ttagatattc
     4261 accagaaaaa tcgcgcattc atataaccag ttttcttgat accaacggct atcttaatat
     4321 tgatatcgcc agtcctggaa cgaaaattaa tgagcctgaa aaactcttcc gtagattttg
     4381 gcggggagat aattcgcgtc attccgaagg tcagggactt ggtctttctt tagtcaaagc
     4441 gattgccgaa ttgcatgggg gaagtgctac gtatcactat tttaataagc ataatgtgtt
     4501 ccgaattacg ttaccgcaaa gaaattaaca tagggcttca gtacgccata aatcaaccat
     4561 gaaaaaacgc agccacgtcg tgagtattaa cgtggctgcg attgattatg cgcttacatt
     4621 catacgtaac agggattaac ccgcataagc tgccagcatt tcctgcgccg ccagtttacc
     4681 caacgcattc gctgcgaaag gagtgtcgcc ggtgagaact ttacggtcct tatgtactcg
     4741 cccggtgatg tcgtcattaa taatgttcat gcccattttc ttcagctctt cgccgaagta
     4801 ccaggtgaga tgacccggca tatagcctat ctctggcgtt tgtttgtctg cggcgtctgg
     4861 gaatgcgcaa atggaataac cattcagtgg gttatcgccg tggcgaagcg ccagaaaagc
     4921 cgccgggccg tggcaaaggg agataacaaa acggtcattt ttgattgccc actgcaaagc
     4981 agcagccacg tcctggcttt ctggtaaacc aataagtgcg ccatgaccac caggaacaaa
     5041 gattgctgca tattcgctgt ctgcgttgag gctgacgaca acatccgcga tttttttcgg
     5101 attgcggaac aacgatttat gctgctcaaa gaatggcatt actttttcat ctttgtgcgg
     5161 catagcccag tattcaaact tggtcatcag accggaaatg gtcgccactt cgaattcgaa
     5221 acctgcagca tggagatgat acaacggcag caacgtttca atcggatggt taccggtcga
     5281 gaacagtttt ccgttatcgg tcggcaaata acgttcgtcc gcggcgatca ccagaatttt
     5341 atgtttaccg cgatacggtt ttg
//
LOCUS       DAZGJW010000238         5267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZGJW010000238 DAZGJW010000000
VERSION     DAZGJW010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.240"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            49..468
                     /locus_tag="ACHKIK_004803"
     CDS             49..468
                     /locus_tag="ACHKIK_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFO9085812.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            465..1025
                     /locus_tag="ACHKIK_004804"
     CDS             465..1025
                     /locus_tag="ACHKIK_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFO9085813.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDTSSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            1026..1271
                     /locus_tag="ACHKIK_004805"
     CDS             1026..1271
                     /locus_tag="ACHKIK_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO9085814.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            1268..2770
                     /locus_tag="ACHKIK_004806"
     CDS             1268..2770
                     /locus_tag="ACHKIK_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO9085815.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRITRASQATELWGRGSMIALMVAEFIAINPDAELYAIAQGNGT
                     GQATAAAMNLTGTVTRDGIVYACVGGRRYTLPAPKGKKGKELTDELAALINADPDAPF
                     TASSGAGSGDNGAGLKGSLGITARFTGECSVHDVRLNYHDGEATPEGIQVAIAYPKQK
                     AANPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWLAHTG
                     TFGEVQAFGAKRNDFLATCTSIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALP
                     SRMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESDPSYLD
                     VETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWV
                     ELGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            2779..3144
                     /locus_tag="ACHKIK_004807"
     CDS             2779..3144
                     /locus_tag="ACHKIK_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO9085816.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASASSTRVQ"
     gene            3159..3635
                     /locus_tag="ACHKIK_004808"
     CDS             3159..3635
                     /locus_tag="ACHKIK_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO9085817.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            3762..>5267
                     /locus_tag="ACHKIK_004809"
     CDS             3762..>5267
                     /locus_tag="ACHKIK_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO9085818.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSLAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKMARAVGTGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFI
                     NFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPGRGRP"
BASE COUNT         1180 a         1373 c         1621 g         1093 t
ORIGIN      
        1 ggatgctccc cggaaaaaga ccggtaacaa agcggaacag gcccgtgcat gaattacgcc
       61 actgaaaccg atatgcgggc gcgttaccgc gaggatttgc tcaggccgtt actggctgtg
      121 ccccgttcgg atgagccgga cacgcgcaag ctgaaccggg cgctgacaga tgcgtcagcc
      181 cttatcgaca gctatctgtc cgcccgttac acgttgccgc tggaggttat tccggccgtt
      241 cttgttcagc actgttgcgc gattgccttt tattacctgt gcgatcagcg agcctccgat
      301 caggcgcgtg accgttaccg tgaagcgctg gcctggctga aagatgtcat gaacggcaat
      361 gtgccggtcg gtgtggatac gaacggtgcg gcccctgaat ccggggattt accacaggtt
      421 cagtctgatg cagcggtatt cgggcgcaac cagaagggct tcatatgatt acggaaaccg
      481 aacaggcata catcgcccgt atccgtgagt atttcgggaa tgaactggtg tctgttgaca
      541 cgcatcccgg cgactggagc gacagcgtac tgcgcaccat gctgattaac gccccggcga
      601 tctacgttgc ctggctgggt gccggtgaag gccgtacccg tggtcgcctg gtcagtcact
      661 gggtgttcta cgtcatcggc gacatgctca acgggcgtga ggccagccgt cccggactgt
      721 atcagattgt ggcccggctg attgccgtgc ttaacggctt cagaacagaa aaaacctcac
      781 cgctttactt tgaaaaggcg gtcaacggtt acaccgaaac ccaggcggac agtggtgcgg
      841 tgatgtatgc gctttatttc tcctgcgagg aaatgatcgc accgctgacc gataccagct
      901 cgctggacga cttcctgcgt cattacgaaa cctttgtgga gccgcccggt acgccgccgt
      961 ttgaggcaca tattgatttg ccggggaaca cgacaacggc ggtcgaaccg ccggaggaac
     1021 tgtaaatgaa aaccatcttt atcaaacccg cgccgggacg cctgattcgt gatcccgaca
     1081 caatgcgccc gctggcacag gagggggagg aaaagccctt tacgccgttc tggtgtcggc
     1141 gtcttgatga cggcgatgtc attcaggctg aaaaagccgc tgaagaggct ccggctgtat
     1201 ctgcggatgc aacgactgcg acagaaaaac cggtcgccca accggcatcc gataaggaga
     1261 aaccgcaatg attaattttg acgggatcgg taatgaccgc cgtataccgc tgattgaggt
     1321 tgagtttaat aactccatgg ctgtcaccgg cactccggca cagcgtcagt gtgtgctgct
     1381 gtttggtcag gcgagaatga aagagaatgc cgtggacggt gccggtgttc tggatgttcc
     1441 ggtcaggatc acccgtgcgt ctcaggcaac agaactctgg gggcgcggct ccatgattgc
     1501 cctgatggtg gcggaattta tcgccatcaa tcctgatgct gaactgtatg ccattgctca
     1561 ggggaacggc accggacagg caacggctgc ggccatgaat ctgaccggaa cggtcacccg
     1621 tgacgggatt gtttatgcct gcgtgggggg gcggcgttat acactcccgg caccaaaagg
     1681 taagaaaggc aaagaactga ccgacgagct ggccgcgctg atcaatgccg atcctgacgc
     1741 gcccttcacg gcctcctctg gtgcaggaag tggtgataat ggcgcaggtc tgaaagggtc
     1801 tctggggatc accgcccgct ttaccggtga atgctccgtt catgatgtgc gcctgaacta
     1861 tcacgacggg gaagcaaccc cggaaggaat acaggtcgct atagcctatc cgaaacaaaa
     1921 agcggctaac ccggatatca cccgaagcgt ggcgggtatg ggcgatcgcc agtacaacta
     1981 tgtcgtcatg ccctataagg atgatgcaaa cctgaaaatc atcagtgatg agcttctgaa
     2041 acgctgggga ccggccaaaa tgtctgacgg cgtgctgtgg ctggcacata ccggcacgtt
     2101 tggtgaggtg caggcattcg gggcaaaacg caatgatttt ctggcgacct gcacatccat
     2161 tcccaaagcc cctgaaccgg attacctctg ggcagcggcg gtctgtgcca cctgtgcacc
     2221 ctctctggga gcagatcctg cgcgtccgct tcagacgctg gcgcttccgt cacgcatggc
     2281 cccggctccg gccgatcgtc tgacgcgtga agagcgtaac agtctgctgt ggggagggat
     2341 tgccacggtc agtgtggctg ccggtgatgt ggttcagatc gaacgtcagg tgaccatgta
     2401 tcgccagaac gtgtacggcg aaagtgatcc cagctatctg gatgtggaaa ccatctacac
     2461 cctctcctgg ctgcgttatt ccctgcgtac ttttatcaca caacgttttc cgcgccataa
     2521 actggctgat gacgggacgc ccgttcgtgc cggtcagaat atcgtgacgc cggagatcat
     2581 gaagcttcag ttaatttcgc tgggtgaaga gtgggtggaa ctcgggctgg tcgaaaatct
     2641 ggacaccttt aaaaagaatc tgcttgtgga gcgtaatgcg tcagatcgaa accgtcttga
     2701 cgtgttatgc acgccggatc tggttaacca gttccgcttc ctggcagcac agatccgttt
     2761 cattttgtga ggtaaggcat gagcggaaaa cagtatcagg gtacggccac tatccgtgtg
     2821 aacggtcagg aatacgagac gcttgagggg gctacatttt ctccgtctgg ttttgaacgt
     2881 gaagtggtga aaggcgcaaa agtctatggt taccgccaga aaccacgtga agcgacgctg
     2941 gactgcaaat ttccggcagg cggagaaggt tcaccagccg ccgatgaaat caacaactgg
     3001 actgcggtca ctatcgagtt tgtggcggat accggcgaag tccacatgat gacgaaggcc
     3061 tggagcagtg aaccggcctc gcttgacggt ggcggggaga tctccgtgaa gttcgccagc
     3121 gcctccagta cccgtgttca gtgatcagga aaaataaaat gaccacacgt aagaaaaaaa
     3181 cggcagtttc cgaggcagcc gtgatggagg caatccggga agcacttgag ggggctgatc
     3241 cacgcactgc cgggctgaca gagcaactgg caaagggata tgtggatctg cttgatggtc
     3301 tgccgtttgg tgagacccgt gaatatcgtg tcacgttccg ggaactgaca gcgaaagaca
     3361 gtattgacgc agaagcagaa gccgagcgcg tggtggagac aaacaatggc ccgatgctga
     3421 tagcgtctcc gtcgttacgc ggtgttgcac tgctgcgccg tcagattgcc gcagtgggag
     3481 acattgaagg tccgttgtca ccccgtcaga ttggacagtt aagcgagcgc gatctctccc
     3541 gcctgatggc agcggtcagc ctgctggata ccgcgcttgc cggaaagctg gcagctgacc
     3601 gggggcgatc aggcgcagtg tcgggatcag attgaagaag cggcaattat cctggggatg
     3661 gtgacaaaaa gcggtccgga atgggcgctt aacctccccc tgtcgcaact ttaccggcac
     3721 tgccgacaga cagaaaaaat catcagaacg aagcagtaag tatggcccga aatctcagag
     3781 catctctgat agttgatttg ctcggcaata tctcagccaa atcccgccag tggtcacagg
     3841 agctgggggc attctcacgc tccggccagg cggggctggg tggcctggga aatgctgccc
     3901 gccgtgccgg gcaggaaaca gatgtactcg gaagcaggat gcagcgcacg ctggccgggg
     3961 tgcggggcag tatccgccat gtcacttctg attttgaccg tcttcagggc agtattaccg
     4021 gcaccatcgg gcggatcagc aatctttacg gaatgctggc cggtggtgct gctgtatatg
     4081 gctttaaccg gggatttatt cgcccggcag cggagatgga aacctatatg attcgcctga
     4141 actcccttta caaaggggat cgggcgaaaa ctgatgatgt ccgcaggtgg gcaatacaga
     4201 atgcgaagga aaccacctgg gggctggctg gcgtcatgca ggaatatacg tccagtctgg
     4261 gctttggtat gagtgacagg gaggcccgaa attttgtcac catgcttcag gatcagggtg
     4321 ccgtgggcgg ctggtcactg tccgatgcac agggggcatc cctgcaactg aaacagatgt
     4381 atgcaagagg cagtattcag gcggcggatg caaatattct tgccacctac ggcattaatg
     4441 cctaccgcgt gctggctgat cgtctgggtg tggaccagaa agtggtcaga aaacttggtg
     4501 aaaaagggct gcttgggccg gacagtattc gcctgttatt tcagacgctg gcggaacagg
     4561 ccaggggagc acaaaaggac gcaatgaatt cctggagtgg cctgacggcc atgatgggtg
     4621 acgtctggga tcagtttgcc cgggatgtga tggacagtgg gccatttgaa aaactgaagg
     4681 gtaatctcag gggatttctt tcatgggttg acagtgccaa atctgatggc agttaccatt
     4741 ccctggcgga aagcacggca tcagccatga accagggctt tgagtatgcc cgtgatgcag
     4801 tgacagggtt ttatcaggcg atcagaaagg tacgcgacac gcttcaggct ctgcgggatg
     4861 cgggctatgg tgatgcgctg gatcgcattg gtcagggggc acagaccgcc gcgaaatacc
     4921 tgctgtacat gtatctcgcc acccgtgccc tgaaaatggc gcgggctgtc ggaacgggga
     4981 ttatacgtcc gggtgctgca ttgctgggat acggattatc agcggcggcc ttcctgactt
     5041 cacctttccg gcgctctcct ccggccggaa caccaccagg cacatctcag ggacgagggc
     5101 aacgttttat aaactttctt accggggtaa atccggcagc cgttcagcca gtacttgtaa
     5161 caaactggcc cacaggagga ctgtccggca ccggcgcgac cgatacttcc tcaggcggac
     5221 gtcaggggcg gggccgcagg aaacgcggac cggggcgcgg aagaccg
//
LOCUS       DAZGJW010000239         5134 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZGJW010000239 DAZGJW010000000
VERSION     DAZGJW010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.241"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..193
                     /locus_tag="ACHKIK_004810"
     CDS             <1..193
                     /locus_tag="ACHKIK_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085819.1"
                     /translation="MDLNIEDSSSEELLSDNTLEKDENSPDNGFEVGEYNTHEAYNSE
                     NQYFTREDYTYNYDLLNAI"
     gene            complement(588..964)
                     /gene="rnpB"
                     /locus_tag="ACHKIK_004811"
     ncRNA           complement(588..964)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKIK_004811"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(997..2113)
                     /gene="garK"
                     /locus_tag="ACHKIK_004812"
                     /pseudo
     CDS             complement(997..2113)
                     /gene="garK"
                     /locus_tag="ACHKIK_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
     gene            complement(2210..3100)
                     /gene="garR"
                     /locus_tag="ACHKIK_004813"
     CDS             complement(2210..3100)
                     /gene="garR"
                     /locus_tag="ACHKIK_004813"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO9085820.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3130..3900)
                     /gene="garL"
                     /locus_tag="ACHKIK_004814"
     CDS             complement(3130..3900)
                     /gene="garL"
                     /locus_tag="ACHKIK_004814"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO9085821.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3916..>5134)
                     /gene="garP"
                     /locus_tag="ACHKIK_004815"
     CDS             complement(3916..>5134)
                     /gene="garP"
                     /locus_tag="ACHKIK_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO9085822.1"
                     /translation="AGTEVAKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSK
                     KVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWF
                     PTKERGTASAIFNSAQYFSLALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLI
                     HNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFF
                     GQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIK
                     RGLSLTLARKLPIVLGMLLASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISD
                     TAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYL
                     FVVGDIKRMELQK"
BASE COUNT         1258 a         1460 c         1221 g         1195 t
ORIGIN      
        1 tatggattta aacatagaag actctagctc agaagagtta ctttcagata acaccctcga
       61 gaaagacgaa aattctccgg acaatggttt tgaggtcggg gaatataaca cacatgaagc
      121 atataactca gagaatcaat attttaccag agaggactat acgtataatt acgacctttt
      181 aaatgcaata tagacttaat aacttcttac aaagtctatt attaataccg tagatattta
      241 ttcatcttac cctgtgcacc aaaagcaaaa actcactgac gcgggtttga aactgatggc
      301 aaccgcaaaa atgcctgatg cgctacgctt atcaggccta cgccatctct gcaatatatt
      361 gaatttgcgt gcttttgtag gcaggataag gcgttcacgc cgcatccggc atgaataaag
      421 cgcactcaac aatctgaaac ccgccggaac ggtttattac gtacatcagg tgaaactgac
      481 cgataagccg ctttcttttg ggtatagcgt cgtggacagt cattcatctt tctgcccctc
      541 caaaagcaaa aacccgccgg tttacgctgg cgggttttcg ttaaatcagg tgaaactgac
      601 cgataagccg ggttctgtcg tggacagtca ttcatctagg ccagcaatcg ctcactggct
      661 caagcagcct acccgggttc agtacgggcc gtaccttatg aacccctatt tggccttgct
      721 ccgggtggag tttaccgtgc cacggactgt taccagccgc gcggtgcgct cttaccgcac
      781 cctttcaccc ttacctgatc ccgcttgcgc gggccatcgg cggtttgctc tctgttgcac
      841 tggtcgtggg tttccccccc aggcgttacc tggcaccctg ccctatggag cccggacttt
      901 cctcccctcc gcccgtctcc cccgaagagg acgacgacga agcggcgact gtctggtcag
      961 cttcggcgcg cagtatagag ggtttgcgcg cccttgtcac cccgcgttgc gcattccaat
     1021 cgccagtgtc gcggcgatat tacgtgaagc acggcagata ttgtcataag ccccgcggaa
     1081 tgcttcgtcc aacgtaccaa tgctggtcag tacgctgaag acagcatcaa tgccatgctg
     1141 atgtacaacg ccaacatcat cggtcaggct acccgcaatg ccaatcaccg gtttatggta
     1201 cttcttcgcc acgtttgcga cgccaatcgg taccttgccg tgaatactct ggctgtcaat
     1261 acgcccttca ccggtgatca ccagcgtaca atcgtgaata tgctcctcca gattcagcgc
     1321 cgtagtgacg atttcaatac cacttttcag ttccgcacca agaaacgcca ttagcgccgg
     1381 ggacatcttt cacatcaaca tgcagcgctt ttttaatgac ctcggcatag tgagagaggt
     1441 tattgtccag ctcaacaatc atcgcttcac tggctccctt ttgtgggcca aagatgcgcg
     1501 atgcgccgtt atcgcccacc agcggattgg tgacatcaca agcgacgcga atgacgcaat
     1561 cttttaagcg cggatcgagg ccggaaatat caatatcatt cagagtatta agactaccgc
     1621 cgccaaaacc aatttcattg ccgttggcgt cgcataattt cgcccccagc gcctgtacca
     1681 tgcctgcgcc gccatcattt gtagcgctac cgccaatgcc gataataatg tttgtcgcgc
     1741 cgctctccag cgcctgcagg attaactcgc ctgtgccgcg tgaagtggtc acgagtggat
     1801 cgcgtttttc cgcaggtacc agctccagcc cactggccgc cgccatttca ataaacgcgg
     1861 ttttgccatc gccggagatc ccccaactgg cattcacttt ctcgcccagc ggccctgtaa
     1921 cccaggcgtg acgttcagaa ccctgggtgg ctgcaatcat cgcttccacc gttccttcgc
     1981 caccgtcggc aaccggaata gaaacgtact gtgcatcagg aaaaatttcc cgaaatcctt
     2041 tttctatcgc ctgcgcaacc tcgctggcag ataaactttc tttataagag tctggggcga
     2101 ttacgatttt catacctatg cctgttacca catgacgccg gagggcgttt ctcttattcg
     2161 gcctggattc caggcccgga ttgcaatacg ccatccgggc acgacgtcat taacgagtaa
     2221 cttcgacttt cgccagtttt tcgtagtagc acgccagggc gctatgatcc gccgttccta
     2281 aaccatctgc tcgcagtgcc tgcatcatct ccataaccgc agctgtgagc ggcagttgcg
     2341 cgccgacgcc gtgggaagta tccagcgcat ttgccagatc cttaatatgc agatcaatac
     2401 ggaagcccgg cttgaagttg cggtccatca ccatcggcgc tttggcatcc agcacggtac
     2461 tgcccgccag tccaccgcga attgcctgat acaccaggtc cgggttaacg cccgctttgg
     2521 ttgccagcgt tagcgcttct gacatcgcgg caatattcag cgccacaatg acctgatttg
     2581 ccagtttggt gacgttacct gcaccgattt ccccggtatg caccacggaa cccgccatcg
     2641 ctttcatcaa atcatagtat ttgtcgaaaa tagccttgtc gccgcccacc atcactgaca
     2701 gcgtaccgtc gatggctttc ggttcaccgc cgctcaccgg agcatccagc atatcaatgc
     2761 ctttcgcttt cagcgcttcg ctgatttcac ggcttgccag cggtgcgata gaactcatat
     2821 cgatcaatac cgtacctggc ttcgcgcctt caataatgcc attctcaccc agcgccacct
     2881 ctttcacatg aggggagttt ggcagcatgg ttatgatgac gtcgcactgt tcagcgatcg
     2941 ctttagccgt agacgctgtt tctgcacctg cagcaatcac gtcagcaata gcttctgggt
     3001 tacggtcagc aaccaccagc gagtaacctg ctttcagaag gtttttactc attggtttac
     3061 ccataatccc caggccaata aaaccaactt tcatagtcat atcaatcatc tctcttgttg
     3121 cggtggtggt tattttttaa aggtatcagc cagtttctga gtggcagagc ggaagacgcc
     3181 aagatcgctg ccgacagcca caaacgtcgc gccccattcc agataacgac gcgcatcggc
     3241 ttcgaccggc gcgagaatac cgctgggttt gccgtgcgca ctggcacggt taaaaatgtg
     3301 ctgaattgct ttttgtacat ccgggtgcga ggcattgccg agatggccta atgccgcagc
     3361 cagatcgctg gggccgacga agataccgtc tacaccttcg gtagcggcaa tggcatcgac
     3421 gttatctacg ccctgctgac tttctatctg gaccagaatg gtgatgttct tgttcgactg
     3481 agcgaaataa tccgccacgg tgccaaacat attggcgcgg tgagaaacgg agacgccgcg
     3541 aatgccttcc ggtgggtaac gggttgatgc caccgcctgc tctgcttcct cttttgtttc
     3601 tacaaaagga atcaggaagt tatagaaacc gatatccaga agacgcttaa taattaccgg
     3661 ctcgttggtc ggcactcgca ctactggcgc gctggcgctg cctttcaggg ccattaactg
     3721 cggaataaac gtggagatat cgtttggcgc atgttcgcca tccagcacca gccagtcaaa
     3781 cccagccaaa ccaagaactt cagtgctaat cgggttagag agtgctgacc agcaaccaat
     3841 ttgtacctgt ttcgcagcca gtgcggcttt gaatttattc gggaaaacat cgttattcat
     3901 cgcttatacc tttgcttatt tctgcaattc catacgttta atgtcgccaa ctacgaagag
     3961 gtagcagacc atcgccatca gcgctgaaca tcccacgaaa accagtgctg cattgaagga
     4021 gtgcagttca cttaccaagt agccaatcac cagtggagtg acaatggagg caacattgcc
     4081 aaagacgtta aagacgccgc cacagaggcc aacaatctct ttcggcgcgg tgtcagaaat
     4141 caccggccag cccagagcac caaatccttt gccaaaaaaa gccagcgcca tcagcatgac
     4201 caccagcgtg gtgttgttgg tgtagttaca taagatgatg gtggaagcca gcaacattcc
     4261 cagcacaatc ggtagcttac gtgccagggt cagggataaa ccgcgtttga tcagataatc
     4321 cgagaagaca cctcccagca cgccgcccgc aaaaccacac agtgccggaa tcgaggcgac
     4381 cagacccact ttcagaatcg acatgccttt ttcctgcacc agataaatcg ggaaccaggt
     4441 gaggaagaac caggtgatgg tgttgataaa atattgtccg aaaaatacgc ccaacatcat
     4501 gcggttagag agcaattgct tgatgtaatg cagtttggga ccgcttgctg ccgcgctgcc
     4561 cggctttttg tggtccatat cgaccaccgc gccattttca gagataaact tcagctcttc
     4621 cgcagacata cgtgggtgat ctgtcgggtt atgaatcaat ttgatccaca gcgccgtcag
     4681 cacaaaacca atcaccccca taacggtaaa gacgtgctcc cagccccagg cgaaagtcag
     4741 ccagccaagc agaggcgaaa agagcgccag cgagaaatat tgcgccgagt taaagatggc
     4801 ggaggcagta ccacgttctt tcgtcgggaa ccaggcggcg acaatacggg cgttcgccgg
     4861 gaatgatggc gcttccgaga agccgagcat aaagcgcata aagaacatgg agatccctgc
     4921 ccaggccagc gggaacatat caacaaagcc ttgcaggaaa gtgaatagcg accagaaaaa
     4981 gaggctatag gtataaactt ttttcgagcc aaacttatca agcagccagc cgccggggat
     5041 ttgcatcagc aagtaggccc agccaaaagc agaaaagatg taacccatcg aaaccgcact
     5101 taactgcaac tcttttgcca cttcggtacc agca
//
LOCUS       DAZGJW010000240         5123 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZGJW010000240 DAZGJW010000000
VERSION     DAZGJW010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.242"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..834
                     /locus_tag="ACHKIK_004816"
     CDS             <1..834
                     /locus_tag="ACHKIK_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO9085823.1"
                     /translation="SRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRR
                     RRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVANNITQSG
                     GTEWSKRDKSTYDLTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKLDTRRGS
                     HSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGNTQARGL
                     RTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDAFVSVQL
                     A"
     gene            876..1271
                     /locus_tag="ACHKIK_004817"
     CDS             876..1271
                     /locus_tag="ACHKIK_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO9085824.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            1283..1657
                     /locus_tag="ACHKIK_004818"
     CDS             1283..1657
                     /locus_tag="ACHKIK_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO9085825.1"
                     /translation="MADFDNLFDAAIACADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRRWKGDVWP"
     gene            1648..2226
                     /locus_tag="ACHKIK_004819"
     CDS             1648..2226
                     /locus_tag="ACHKIK_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9085826.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSVSQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            2223..2618
                     /locus_tag="ACHKIK_004820"
     CDS             2223..2618
                     /locus_tag="ACHKIK_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO9085827.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSELITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            2626..3366
                     /locus_tag="ACHKIK_004821"
     CDS             2626..3366
                     /locus_tag="ACHKIK_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9085828.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATDMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTDS"
     gene            3382..3804
                     /locus_tag="ACHKIK_004822"
     CDS             3382..3804
                     /locus_tag="ACHKIK_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO9085829.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKQPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            3786..4220
                     /locus_tag="ACHKIK_004823"
     CDS             3786..4220
                     /locus_tag="ACHKIK_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO9085830.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            4213..>5123
                     /locus_tag="ACHKIK_004824"
     CDS             4213..>5123
                     /locus_tag="ACHKIK_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO9085831.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARA"
BASE COUNT         1134 a         1312 c         1641 g         1036 t
ORIGIN      
        1 tcccgtggcg gctccacctc tgaatttacg ccgggatatg tcaaacccaa gcatgaagtg
       61 aatccgcaga tgaccctgcg tcgcctgccg gatgaagatc cacagaatct ggcggacccg
      121 gcttaccgcc gccgtcgcat catcctgcag aacatgcgag acgaagagct ggccattgct
      181 caggtcgaag agatgcaggc agtttctgcc gtgcttaagg gcaaatacac catgaccggt
      241 gaagccttcg atccggttga agtggatatg ggccgcagtg tggcgaacaa catcacgcag
      301 tccggcggta cggagtggag caagcgtgac aagtccacgt atgacctgac cgacgatatc
      361 gaagcctacg cgctgaacgc cagcggtgtg gtgaatatca tcgtgtttga tccgaaaggc
      421 tgggcgctgt tccgttcctt caaagccgtc aaggagaagc tggatacccg tcgcggctct
      481 cattccgagc tggagacagc ggtaaaagac ctgggcgaag cggtgtccta taaggggatg
      541 tatggcgata cggcgatcgt cgtgtattcc ggacagtacg tggaaaacga cgtcaaaaag
      601 aacttccttc cggacaacac gatggtgctg gggaacactc aggcacgcgg tctgcgcacc
      661 tatggctgca ttcaggatgc ggacgcacag cgcgaaggta ttaacgcctc tgcccgctac
      721 ccgaaaaact gggtgaccac cggcgatccg gcgcgtgagt tcaccatgat tcagtcagca
      781 ccgctgatgc tgctggctga ccctgatgcg ttcgtgtccg tacaactggc gtaatcatgg
      841 cccttcgggg ccattttctc tctgtggagg agtccatgac gaaagatgaa ctgattgccc
      901 gtcttcaggt gctgggtgag caactgaacc gtgatgtcag cctgacgggg acgaaagaag
      961 aactggcact ccgtgtggca gagctggaag aggagcttga tgacacggat gacgctgccg
     1021 gtcaggacac atctgtcagc ccggaaaatg cgctgaccgg acatgaaaat gaggttgtat
     1081 cagcgcagcc ggataccgtg actgatacgg ctgatctggt cacggttgtg gcactggtga
     1141 cgctgcatac tgatgcactt cacgccacgc gggatgaggc tgtggcattt gtgctgccgg
     1201 gaacggcgtt ccgtgtctct gccggtgtgg cagctgaaat gacagagcgc ggcctggcca
     1261 gaatgcaata acgggaggcg ctgtggctga tttcgataac ctgttcgatg ctgccattgc
     1321 ctgcgccgat gaaacgatac gcgggtacat gggaacgtca gccaccatga catccggtga
     1381 gcagtccggt gctgtgatac gtggtgtttt tgatgaccct gaaaatatca gctatgccgg
     1441 acagggcgtg cgcgttgaag gctccagccc gtccctgttt gtccggactg atgatgtgcg
     1501 gcagctgcgg cgcggcgaca cgctgaccat cggtgaggaa aacttctgga tagaccggat
     1561 ttcgccggat gatggcggaa gctgtcatct ctggcttggg cggggcgtac cgcctgccgt
     1621 taaccgtcgc cgctggaagg gggatgtatg gccataaaag gtcttgagca ggccgttgaa
     1681 aacctcagcc gtatcagcag aacggcggtg cccggtgccg ccgcaatggc cattaaccgc
     1741 gttgcttcat ccgcgatatc gcagtcggtg tcacaggttg cccgtgagac aaaggtacgc
     1801 cggaaactgg taaaggaaag ggccaggctg aaaagggcca cggtcaaaaa tccgcaggcc
     1861 agaatcaagg ttaaccgggg ggatttgccc gtaatcaagc tgggtaatgc gcgggttgtc
     1921 ctttcgcgcc gcaggcgtcg taaaaagggg cagcgttcat ccctgaaagg tggcggcagc
     1981 gtgcttgtgg tgggtaaccg tcgtattccc ggcgcgttta ttcagcaact gaaaaatggc
     2041 cggtggcatg ttatgcagcg tgtggccggg aaaaaccgtt accccattga tgtggtgaaa
     2101 atcccgatgg cggtgccgct gaccacggcg tttaaacaaa atattgagcg gatacggcgt
     2161 gaacgtcttc cgaaagagct gggctatgcg ctgcagcatc aactgaggat ggtaataaag
     2221 cgatgaaaca tactgaactc cgtgcagccg tactggatgc actggagaag catgacaccg
     2281 gggcgacgtt ttttgatggt cgccccgctg tttttgatga ggcggatttt ccggcagttg
     2341 ccgtttatct caccggcgct gaatacacgg gcgaagagct ggacagcgat acctggcagg
     2401 cggagctgca tatcgaagtt ttcctgcctg ctcaggtgcc ggattcagag ctggatgcgt
     2461 ggatggagtc ccggatttat ccggtgatga gcgatatccc ggcactgtca gagttgatca
     2521 ccagtatggt ggccagtggc tatgactacc gacgcgacga tgatgcgggc ctgtggagtt
     2581 cagccgatct gacttatgtc attacctatg aaatgtgagg acgctatgcc tgtaccaaat
     2641 cctgtaatgc cggtgaaagg tgccgggacc accctgtggg tttataaggg gagcggtgac
     2701 ccttatgcga atccgctttc agacgttgac tggtcgcgtc tggcaaaagt taaagacctg
     2761 acgcccggcg aactgaccgc tgagtcctat gacgacagct atctcgatga tgaagatgcg
     2821 gactggactg cgaccgggca ggggcagaaa tcagccggag ataccagctt cacgctggcg
     2881 tggatgcccg gagagcaggg gcagcaggcg ctgctggcgt ggtttaatga aggtgatacc
     2941 cgtgcctata aaatccgctt cccgaacggc acggtcgatg tgttccgtgg ctgggtcagc
     3001 agtatcggta aagcggtgac ggcgaaggaa gtgatcaccc gcacggtgaa ggtcaccaat
     3061 gtgggacgcc cgtcgatggc agaagatcgc agcacggtca cagcggcaac cgacatgacc
     3121 gtgacgcctg ccagcacctc ggtggtgaaa gggcagagca ccacgctgac cgtggcattc
     3181 cagccggaag gcgcaaccga caagagcttc cgtgcggtgt ctgcggataa aacaaaagcc
     3241 accgtgtcgg tcagtggtat gaccatcacc gtgaaaggtg ttgctgcagg caaggtcaac
     3301 attccggtcg tatccggtaa tggtgaactt gctgcggttg cagaaatcac tgtcaccgac
     3361 agttaatccg gagagtcagc gatgttcctg aaaaccgaat catttgaaca taacggcgtg
     3421 accgtcacgc tttctgaact ttcagccctg cagcgtattg agcatctcgc cctgatgaaa
     3481 cggcaggcag aacaggcgga gtcagacagc aaccggaagt ttacagtgga agacgccatc
     3541 agaaccggtg cttttgtggt ggcgatgtcc ctgtggcata accatccgca gaagacaaag
     3601 cagccttcca tgaatgaagc cgttaaacag attgagcagg aagtgcttac cacctggccc
     3661 acagaggcaa tttctcatgc tgaaaacgtg gtgtaccggc tgtccggtat gtatgagttt
     3721 gtggtgaatg atgcccctga acaggcagag gacgccgggc ctgcagagcc tgtttctgcg
     3781 ggaaagtgtt cgacggtgag ctgagttttg ccctgaaact ggcgcgcgag atggggcgac
     3841 ccgactggcg tgccatgctt gccgggatgt catccacgga gtatgccgac tggcaccgct
     3901 tttacagtac ccattatttt catgatgttc tgctggatat gcacttttcc gggctgacgt
     3961 acaccgtgct cagcctgttt ttcagcgatc cggatatgca tccgctggat ttcagtctgc
     4021 tgaaccggcg tgaggctgac gaagagcctg aagatgatgt gctgatgcag aaagcggcag
     4081 ggcttgccgg aggcgtccgc tttggcccgg acgggaatga agttatcccc gcttccccgg
     4141 atgtggcgga catgacggag gatgacgtaa tgctgatgac agtatcagaa gggatcgcag
     4201 gaggagtccg gtatggctga accggtaggc gatctggtcg ttgatttaag tctggatgcg
     4261 gccagatttg acgagcagat ggccagagtc aggcgtcatt tttccggtac ggaaagtgat
     4321 gcgaaaaaaa cagcggcagt cgttgaacag tcgctgagcc gacaggcgct ggctgcacag
     4381 aaagcgggga tttccgtcgg gcagtataaa gccgccatgc gtatgctgcc tgcacagttc
     4441 accgacgtgg ccacgcagct tgcaggcggg caaagtccgt ggctgatcct gctgcaacag
     4501 ggggggcagg tgaaggactc cttcggcggg atgatcccca tgttcagggg gcttgccggt
     4561 gcgatcaccc tgccgatggt gggggccacc tcgctggcgg tggcgaccgg tgcgctggcg
     4621 tatgcctggt atcagggcaa ctcaaccctg tccgatttca acaaaacgct ggtcctttcc
     4681 ggcaatcagg cgggactgac ggcagatcgt atgctggtcc tgtccagagc cgggcaggcg
     4741 gcagggctga cgtttaacca gaccagcgag tcactcagcg cactggttaa ggcgggggta
     4801 agcggtgagg ctcagattgc gtccatcagc cagagtgtgg cgcgtttctc ctctgcatcc
     4861 ggcgtggagg tggacaaggt cgctgaagcc ttcgggaagc tgaccacaga cccgacgtcg
     4921 gggctgacgg cgatggcacg ccagttccat aacgtgacgg cggagcagat tgcgtatgtt
     4981 gctcagttgc agcgttccgg cgatgaagcc ggggcattgc aggcggcgaa cgaggccgca
     5041 acgaaagggt ttgatgacca gacccgccgc ctgaaagaga acatgggcac gctggagacc
     5101 tgggcagaca ggactgcgcg ggc
//
LOCUS       DAZGJW010000241         5013 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZGJW010000241 DAZGJW010000000
VERSION     DAZGJW010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.243"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(116..874)
                     /gene="chbG"
                     /locus_tag="ACHKIK_004825"
     CDS             complement(116..874)
                     /gene="chbG"
                     /locus_tag="ACHKIK_004825"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO9085832.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(887..2239)
                     /gene="celF"
                     /locus_tag="ACHKIK_004826"
                     /gene_synonym="chbF"
     CDS             complement(887..2239)
                     /gene="celF"
                     /locus_tag="ACHKIK_004826"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO9085833.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(2344..3186)
                     /gene="chbR"
                     /locus_tag="ACHKIK_004827"
     CDS             complement(2344..3186)
                     /gene="chbR"
                     /locus_tag="ACHKIK_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO9085834.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(3194..3544)
                     /gene="chbA"
                     /locus_tag="ACHKIK_004828"
     CDS             complement(3194..3544)
                     /gene="chbA"
                     /locus_tag="ACHKIK_004828"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO9085835.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEVKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(3595..4954)
                     /gene="chbC"
                     /locus_tag="ACHKIK_004829"
                     /pseudo
     CDS             complement(3595..4954)
                     /gene="chbC"
                     /locus_tag="ACHKIK_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
BASE COUNT         1376 a         1315 c         1062 g         1260 t
ORIGIN      
        1 agcgcatcag gcagttttgc gtttgtcagc agtctcaagc ggcgcagtta cgccgccttt
       61 gtaggaatta atcgccggat gcaaggttca cgccgcatct ggcaaacatc ctcacttaca
      121 catcccgata actccccaac cgataaccac gctgagcgat agcacctttc aacgacgctg
      181 atgtcaacac atccagctcc gttaagcgtg ggaaacagta agcactctga cggatagtat
      241 tatcgataaa cgccggatga cacatcacct ccagcgaacg atcaccccga tggcttgcgt
      301 catccagcac ttgcaggaac agtgcctcgc taatctcttc accataaaac gcgctactga
      361 atccctgaga actgcgcaga ttagccggta aatcaccagc gttagatagc ggctgacggt
      421 cgatgcgcaa cgcaatcccc tcttcagccg caaacctggc aacaatcggg aaaatctgcg
      481 ggaacatatg cacatgatga tggctatcaa gatgcgtagg tttgcgtcca aatagctcaa
      541 tgaaacgcaa atactgactg gcaagctcct gagtaatttc ttccagcggt aaagcatctt
      601 cttctgccaa ctgccagatc cattttccca gcacaccatc gcgggttaac cccggcatag
      661 ctgtcagggg cttgcccata gtaaggacaa agtgcatccc tatggccaga ctcggttcat
      721 cacgactcaa ctgcaccgca tggtcaatag cctgcccatt caccagcgcc gtcgttgatg
      781 tgacaatccc attgcgacag gcctcgataa tgccgtagtt ctgacctttg cttaagccaa
      841 aatcatcggc attaacaatc agtaagcgtt ccataatcag cctcggttaa tgtgcttttt
      901 taagctctgc gatgcagtcg gcaaaatttg gcagccattt ctcgtgcgcc agaatcatct
      961 cgcgtgccag cagctcagca tcgcgatcgg aatgcaccaa cggactaagg tttagcgcca
     1021 gtaacacatc gttaaattct ccgctaagtg cggcgttgct ggcagcaatc tcgaagcctt
     1081 taatggtgtg aatcagcccc atcactttat catcgaaatg cgtaatgcgc ggatgtggcg
     1141 tcgcgccatc gcgccccagc ttacaggtca tttctaccgc ccagtctgcc ggaatattat
     1201 caatatgccc atgatgcggg atattaacgt aatgttcagc ttgcttgtcg ttgtagatag
     1261 cgttgatcac ttcgcacgct gcatcagagt aataagcccc accgcgctgt tccagttctt
     1321 tcggcttaac tttcaactcc ggatttttat acagctcaaa aagttgtttc tcgactttct
     1381 gtactacctg tgctcgtgcg ccgcctttgt agtattcgcc catttcaata gccagcatct
     1441 ctttctgctt gaagtaatac agcagataag aacatggcag cagatttaac gaacgaatta
     1501 agccctcact aaatggcaga tcgaaaatat tttttacaga ggacgctttt aactgccctg
     1561 acgccacacc atcaagcaat tcggcaaagc gcgacttgcc atttaccagc acatccttaa
     1621 tgaacaccat atggttgagg ccgaacagat cgatagataa atcatcgctg tctttcagca
     1681 tcagaacatc gcgaataaac atcttcatgc cgatcggaat attacacacg ccgataaagc
     1741 gtttaaatcc ggtatgacga taaacggctt cagtgaccat tcccgccggg ttagtgaagt
     1801 taatcaccca tgcattcgga caaagttctt cgacatcttt tacgatgtca aaaatcaccg
     1861 gaatggtacg cagaccttta aacagaccgc ccgcgccgtt ggtttcctga ccaagataac
     1921 catgacttaa tggaatacgt tcatccagtt cgcgcgccgg taattggcct acgcgcagtt
     1981 gggtagtaac gaaatcagca tctttcaatg cttcgcggcg atccagcgtt ttataaagct
     2041 tcatcgggac gccagcgtta tcaatcatcc gttggcagag ttcaaaaatg atatccagtt
     2101 tctctttacc atcttcgaca tccaccagcc ataattcgct gaccggcaat tcgtgataac
     2161 gcttaataaa tccttccagt aactccgggg tatagctgct cccgccacca atagtgacga
     2221 cttttaattt ctggctcata atttctccct tcagtacaga atactgatat ctggcatatc
     2281 tgcccccccg gacataaata atccagcaac aggacagata tgtgaattgt caggtataac
     2341 gacttactga ttaaattcag tcaatttctt acgatagctc ttaggcgtaa aggacgttaa
     2401 tttcttaaac gttttaataa acaaactcgg gctactataa ccggcctcaa acgcaatatc
     2461 cgtcacggaa tagttggtca tttccagttg ttttttggca aaattaatac ggatctcatt
     2521 aataatctgc attggtgttt tgccataata tcgttgagtg gctcgcgtca aatattcctg
     2581 tgatttggct gacaacgtca ccatattctc cagcgccgat tcactaaact gctctttatc
     2641 atgcatcttt tctaccgtac ttttcagcca ctgcggtaca tcatcaatca cctgttcttc
     2701 gcggtaatga cgtaaacggt taatgacata aaaagtaacc atctcaacaa actcttccag
     2761 ccctgtttcg cggaaattca atgaagaaat cactgtttcc acataggtga gaaaagcgtt
     2821 attggtccgg tatacctgcg aagcgacaaa gcaataaggc aacaacggca ggtaatgctg
     2881 ctcaaaaaag cgtttactga tcccaacgtt caatatacgc gtggcaccaa actcataaaa
     2941 actttgatgg tgcgaaccta acggaataaa aacaaaatcg ccccgttcca gtaacacgcg
     3001 cttaccgtta atctcctgga aataacgccc ggttaatacc agagtaaatt cataatagtc
     3061 gtgctgatgc agcccgctga tactctcagt tttgttatag ataaacacat ggaagttttt
     3121 gccattaaac aactgctgtt ctcgggcagt ggcaatttcc ggcgcgttaa tcactggctg
     3181 catcatcgac tccttatgcc ttcagttttt catgaagctc aattaattca gtaatcagtt
     3241 cacgcgcaag catggacgtc attaaatgat cctgagcgtg gaccagcacc agactcactt
     3301 tcatcttaac ttcgcccgca tcgccttcaa tcagtttcgt ctgtaccaga tgcgcttcat
     3361 tcaatgccat ccgtgactga tccatcatgg cttttgctgc agcaaaatcg ccctgcttcg
     3421 cctgtttcag tgccgcatac gccaggctgc gcgcttgtcc ggagttgatg ataagcccca
     3481 tcaccacttc ttccagctct tcagcttctg tttgcgtatc aggaatatta tcgagatcca
     3541 tcatacatcg ttcctctctc ttcttaccgg caagattacc cgtaccggca tcgattaaaa
     3601 tttcagggcg ttagcgatat cttcttcgct ctcttcttta tcaatcgcat tttgtgcttt
     3661 gttagccacc acaacaaagg gcagataaat taaggttgcg atgccaaggt tgaagagtgc
     3721 gaccagcaat gcggcgacgc taccgttggt gttaaagaag gctcccagac cggttggcat
     3781 ggtccacggt gcaatattgg tcaccggagg aatgatgccc atgtagtacg ctgcgagggt
     3841 gattgccgcc agaatcggtt gtaccagtac aaacgggata aacatcaccg ggttcatgat
     3901 aattggcaga ccaaacagaa tcggttcgtt aatctggaag atgccggacg gcagcgccag
     3961 ttttgccacc tgacgataat cagcacggcg agaggcgata aagatagcca ggatcaggcc
     4021 taaagtcgca ccactgcccc caaggaaaat aaaggagtcc agcatcggct tggcccagat
     4081 atggaaggtc ttaccggctg ccagcgccgc ttcgacggaa ccatattgct gataggtcgc
     4141 gatattttcc agcgcccacg gcgtcataat gccgttgtcc agtgcggtca gcgccagcgc
     4201 gccatgaata ccgaagaacc agagcagtgg aacaaagatc acataggccc agcccaccac
     4261 gctacccaac gatgccagtg gggttgagat ggtatccata atgatctgat ggaagttggt
     4321 gccccaggta ttcaacgccc aggcaataat ccccatcacg gaaagaataa taaagccggg
     4381 aattaatgcc gagaaggaac gcgatactga agcaggtacg ctgtcgggca gtttaatgac
     4441 ccaattgcgg cggacaataa aggtaaacat ttctgccacc accaggccaa taataatccc
     4501 ggagatgata ttcgccccac ctaaccagtt tgcgccaacc gcataggcct caccgacact
     4561 atatgggggt gacggtcata aatgctgcaa cggataacaa ccccgccgcc agcgcatcga
     4621 ctttacgctc ttctgccagc gccatgccaa taaagaacgg tgccattaaa gacattatcc
     4681 ctaatgttcc gttatatacg ttgccgccaa tacctttcag gccattaagt gtttcaatgg
     4741 ttgaggcgtc gaggcgaata cctaaggaat aaaaaaacga cccctcccca aagcttagaa
     4801 aaacgttgtt aattaataca aacatggccc ccgcaagggt taacggcatt aagcgaataa
     4861 agccattttt gattgcatta acgtgtggct gctttcctat tttaactgca aaagggagga
     4921 gtaccttttc aagcgatgca ataacattac tcatagaaaa atacccttaa aaaccgcaat
     4981 ttaaatattg cggtattgat ttatgaaata act
//
LOCUS       DAZGJW010000242         5010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZGJW010000242 DAZGJW010000000
VERSION     DAZGJW010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.244"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            65..781
                     /gene="nanC"
                     /locus_tag="ACHKIK_004830"
     CDS             65..781
                     /gene="nanC"
                     /locus_tag="ACHKIK_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO9085836.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVDVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            801..1907
                     /gene="nanM"
                     /locus_tag="ACHKIK_004831"
     CDS             801..1907
                     /gene="nanM"
                     /locus_tag="ACHKIK_004831"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO9085837.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWKALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1972..2952
                     /gene="nanS"
                     /locus_tag="ACHKIK_004832"
     CDS             1972..2952
                     /gene="nanS"
                     /locus_tag="ACHKIK_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO9085838.1"
                     /translation="MNAIISPDYYYILTIAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPPCHFNDMIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDKFVEAILQFWRER"
     gene            complement(2960..3610)
                     /locus_tag="ACHKIK_004833"
     CDS             complement(2960..3610)
                     /locus_tag="ACHKIK_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO9085839.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHLDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HVHHLVPLSAIKEDYQLDPVNDLLPVYPNCHAMLHRRKPPFTPEQLKALMDANKSN"
     gene            3747..3890
                     /locus_tag="ACHKIK_004834"
                     /pseudo
     CDS             3747..3890
                     /locus_tag="ACHKIK_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(4554..4682)
                     /locus_tag="ACHKIK_004835"
                     /pseudo
     CDS             complement(4554..4682)
                     /locus_tag="ACHKIK_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(4658..4807)
                     /locus_tag="ACHKIK_004836"
                     /pseudo
     CDS             complement(4658..4807)
                     /locus_tag="ACHKIK_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
     gene            complement(4892..>5010)
                     /locus_tag="ACHKIK_004837"
     CDS             complement(4892..>5010)
                     /locus_tag="ACHKIK_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO9085840.1"
                     /translation="IFDNNEDKTDSAALLPCIEQAREDIRTLRQLQLQHQNR"
BASE COUNT         1441 a         1086 c         1174 g         1309 t
ORIGIN      
        1 ataaatgcta attgtttatg cgggctttgt attgctttct gtttcctaca aatgagtgaa
       61 atttatgaaa aaggctaaaa tactttctgg cgtattatta ctgtgctttt cgtcaccatt
      121 aatttctcaa gctgcgacac tggacgtacg tggtggatat cgtagtggaa gccacgccta
      181 tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa gtatggaaag
      241 taatacctgg aataccattc atgataataa aaaggaaaat gccgcactca atgatgttca
      301 ggttgatgtt aattacgcga ttaaacttga tgatcaatgg acggtgcgcc cgggaatgtt
      361 aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt cctgggacgc
      421 gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt accgacaaca
      481 agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat atgtcactta
      541 ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca aacagaacga
      601 ttatcgctat gcaaaccata agaaatgggc gacggaaaat gcatttgttc tacaatacca
      661 tatgacgccg gatattacgc catacataga atatgactac cttgaccgtc agggtgttta
      721 caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat ttaaactgta
      781 gtagacagga gacagtcaca atgaataaaa caataacggc gcttgctatc atgatggctt
      841 catttgccgc aaacgcgtct gtattaccgg aaactcctgt gccatttaaa agtggtaccg
      901 gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtacg gcatggtaca
      961 agctggatac acaggccaaa gataaaaaat ggaaagcgtt agctgcattc cctggtggac
     1021 caagagatca agcaacctcg gcatttattg atggcaatct gtatgtgttt ggcggcattg
     1081 gcaaaaacag cgagggcttg actcaggtat ttaatgacgt acacaaatac aaccccaaaa
     1141 ccaatagttg ggttaaattg atgtcgcacg caccgatggg catggcgggt catgtaactt
     1201 ttgtacacaa cggcaaggct tatgttactg gcggtgttaa ccagaatatc ttcaatggct
     1261 attttgaaga tctcaacgag gctggaaaag attcaaccgc tatagataaa atcaacgctc
     1321 actattttga caaaaaagca gaagattatt ttttcaataa gtttctgttg tcttttgatc
     1381 cctcaacaca gcaatggagt tacgctggcg aatcgccctg gtacggaacg gctggtgcgg
     1441 cggttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa ccaggattgc
     1501 gaacggatgc cgtatttgaa cttgatttca ccggtaataa tttaaaatgg aataagcttg
     1561 ctcccgtctc atcaccagat ggcgtagctg gcggttttgc ggggataagc aatgattctc
     1621 ttatatttgc cggaggggcc ggattcaaag gttcacgaga aaattaccag aacggtaaga
     1681 actatgcgca tgaaggcctg aaaaaatcat atagcactga tattcatctt tggcataacg
     1741 ggaaatggga taaatcgggt gaattatcgc aaggtcgggc ctacggagta tcattgccct
     1801 ggaataatag tctattgatt attggcggtg aaactgcagg cggcaaagcg gtgacggatt
     1861 cagttttgat ctctgtgaag gataataaag ttacagtaca aaactaacgc ttcagggccc
     1921 cggtaaaggg gccctgctat caatttgtca tttcaattac gcgaaattta tatgaacgca
     1981 ataatatcgc ccgattatta ctatattctt accattgctg gtcagtctaa tgccatggcg
     2041 tatggcgaag gactgccatt accggacagg gaagatgcgc ctcatcccag aatcaaacaa
     2101 ttagcgagat ttgcgcatac gcatcccgga ggcccgccat gtcactttaa cgacatgatt
     2161 ccactgactc actgcccaca cgatgttcag gatatgcagg gttatcacca tcctctggca
     2221 acgaatcatc aaacacagta cggcaccgtt ggccaggcac tgcatatagc acggaaatta
     2281 ctgcccttta ttcctgataa tgcaggggtt ctcatcgttc cgtgttgccg tggcggatcg
     2341 gcttttaccg cgggcagcga agggacatat tcagaacggc acggagccag ccatgatgct
     2401 tgtcgttggg gaacggatac tccgctatac caggatttag tcagcagaac gcgagccgca
     2461 ctggcaaaaa atccgcataa caaattcctc ggcgtatgct ggatgcaagg cgaatttgac
     2521 ttaatgacca gtgactacgc gtcacaccct caacacttta atcatatggt tgaagccttt
     2581 cgtagggatc taaaacaata ccattctcag cttaataata ttactgacgc accgtggttt
     2641 tgcggcgata ccacctggta ctggaaagaa aatttccctc atgcgtatga agttatttat
     2701 ggcaattatc aaaataatgt tttagccaat attattttcg tcgacttcca gcaacaaggt
     2761 gaaagaggac tgacgaacgc gcctgatgaa gacccggacg atttaagcac gggatattac
     2821 ggttcagcgt accggtcacc ggagaactgg acgacggcac tgcgaagcag tcatttcagc
     2881 acggcagccc gtcgggggat tatttctgac aagtttgtag aagcaatttt gcagttttgg
     2941 cgcgaaaggt gagcgttcat taatttgatt tattcgcatc catcagcgct tttaactgct
     3001 ccggcgtaaa cggtggtttg cgtcggtgca acatcgcatg gcaattaggg tacacaggca
     3061 gcaaatcatt aactggatcc agctggtaat cctctttaat ggctgacagc ggcaccaggt
     3121 gatgcacatg gataaagcct tcagcgatat caccatagat cttcaccaga tcaacaccgc
     3181 atacgttgca ctggcatcca tgatgttcga ccgctttatc tcgggccttt ttatcgcgtt
     3241 catagcggtt tactgtcacc tttatcgccg caccttcaag gtatgtctcc tgcgacggca
     3301 actcatccgg gaacagaacg tcggctatgg gagccgcagc ggctttatcc agatgaatat
     3361 gaaatccctg ctctatgagt agcttgacac atttagattt tattccgccg gtgaaatccg
     3421 caggggtaaa ttgcgtcccc gtcatcaatg tcgctgcgat gccagcaatc gccttcgagg
     3481 ggtaacgtcg gccatgatat tcaatctcat acaaccgcgc aggctggaac tgatgagttt
     3541 ctcccgcatc ccataaatgg atagcttcga tgatataccg ccgcggaaga tgatcaggga
     3601 gtttattcat ggagaatgtc cttatacgtt ctcagaacag gcagcaaaaa aacaaatgag
     3661 atcaaggtaa tatatcttca cttgcaggct tgaacagcag aaaatttgcg agcatgatcg
     3721 caattatttc actgccaggg atatcaatag cttccagtaa atcgctacag caggcaattg
     3781 ccaatataaa aatctggcac aagggtgaac agcgcgcgcc gcataagcca ttgttattgc
     3841 tatacgtatt agcgggatac ctgaatggac atccgcgcct tttcgattat tgtagtggaa
     3901 cagtctacga ggatgcagtt cgaagtgagt aagtaaaggt tgcgggaaac gatcaatgcc
     3961 gccatacaat gattttttag gatcaacaat gagtaataaa aactacgaaa gtcaccgcaa
     4021 agcgattgtt agtaaaggta taccacccgc tctattaaat aggctcacca attcggatgt
     4081 tcaggtgatc aataccttct taacacgagt gagcaaactg gaactatctc aacaagagaa
     4141 agactggatc ataaaaatca tctctatggt ttagacaatt tcagccttgt agcctttcaa
     4201 gggttttgtg tgggtacaaa cattatcgaa tcggggtata gccgcagctg tagctgtatc
     4261 cacatcagta agttgatgta ggttgactta ggttgagcga aaaatagcga aaagccttgt
     4321 gtggcgcgga tttcagtcat aaaaaaacgt ccgttgatgt ctattgatgt tccgatggtg
     4381 cgaaggtgcg aaggtgcgaa ggccggactc aaacatgtga cttaacctca tgatttaaaa
     4441 ttgttaataa taaacaatat catcttatac caacacagat accaacacga aaaatcactg
     4501 gttttcggca tcgaaccagg acaatcagct cctgattgag ataccgagct ctgttactcg
     4561 tcatcttcct cgacaggctt cattgcatca agtagtactc gcaccatcgc gatagaacca
     4621 atccggataa aagcatcttc tccatctata tctgttaact gatatcgctc acttgatgcc
     4681 ggataattgt gtcgatttgc agcagtgaac tctttcgtac agccagaatg cggcggatgc
     4741 tggttgagca gataacgtcc gtattctcgt tgaccacttc cggacggtag gctgtgatat
     4801 cagacataat cgatattcct ttttgcagat aaatgaaaat gccagccctg ttcaggctgg
     4861 cgtcagtgga atgaagtctg gtgagtaaga ctcaccggtt ctggtgctga agctgcagtt
     4921 ggcgcagggt acggatatcc tctctggcct gttcgataca gggcaacagt gctgctgaat
     4981 ccgttttatc ctcattgttg tcaaagataa
//
LOCUS       DAZGJW010000243         4993 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZGJW010000243 DAZGJW010000000
VERSION     DAZGJW010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.245"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..170
                     /locus_tag="ACHKIK_004838"
     CDS             <1..170
                     /locus_tag="ACHKIK_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO9085841.1"
                     /translation="HGGERGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            613..4515
                     /gene="espP"
                     /locus_tag="ACHKIK_004839"
     CDS             613..4515
                     /gene="espP"
                     /locus_tag="ACHKIK_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HFO9085842.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVIARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4763..>4993)
                     /locus_tag="ACHKIK_004840"
     CDS             complement(4763..>4993)
                     /locus_tag="ACHKIK_004840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085843.1"
                     /translation="TAQNQPAETVRAAAAATGRFAVAFPVRVAPGSGCFMKTNAPRLF
                     FVDKESGVSFDVFHVLSVSFPVTACATHDGGM"
BASE COUNT         1516 a         1133 c         1113 g         1231 t
ORIGIN      
        1 accatggtgg tgagcgggga gcgctactgt acagcctgat cgggacgtgc aaactgaatg
       61 acgtggatcc agaaagctac cttcgccatg tgcctggcgt catagcagac tggccggtca
      121 accgggtcag cgaactgctt ccgtggcgca tagcactgcc agctgaataa cacatccccg
      181 tcaatacggc cctcgctgta cgcttacgca taaatattga atacatttac tgagcatgca
      241 aaagaaaaag acaaactaat cccggccttt cacagaattt cctctatcag aggctgagtt
      301 gacaacctgc tcacccagga aatagtaaca cccgaaaaca tgaagagact ctgataaaca
      361 atctcgatac atattcctca tctttcacag catctcactt ctttaacagg aattattatt
      421 atctacgcct cctcctttca tgtatccctc atgaatgatt aagataaaca tactgtgttt
      481 gttatccgta tgataacaaa cacataaagg agtaaacatt tatctggatg atgattacaa
      541 tgttcacgac tgtcagagac acacctctga tacattagaa aaaccaatgt ttcccttaaa
      601 aatggagctt atatgaataa aatatactct cttaaataca gccatattac aggagggtta
      661 atcgctgttt ctgaattatc cggcagagta tcatcaagag caactggtaa gaaaaaacac
      721 aaacgcatac ttgcattatg ttttttaggc ttattacaat cctcatattc ttttgcgtca
      781 cagatggata tttcaaattt ctacatccgt gactatatgg attttgcaca gaacaagggc
      841 atatttcagg ctggcgcaac aaatattgaa atagtgaaga aagatggctc caccctgaaa
      901 ctaccggaag taccatttcc tgacttctca ccggttgcaa acaaagggtc aaccacatct
      961 attggtggtg catacagtat cacagccaca cacaatacga aaaaccacca ctcagttgcg
     1021 acgcaaaact gggggaacag cacgtacaaa caaactgact ggaatacttc acatcctgat
     1081 tttgcagtat cccgacttga caagtttgtt gttgagaccc gaggtgcgac tgaaggcgca
     1141 gatatttcgt tatcaaaaca gcaggcactt gaacgttacg gggttaatta taaaggagaa
     1201 aagaaactta tcgcattcag agccggctct ggtgtggtat ccgttaaaaa aaatggacgc
     1261 ataactccat ttaatgaggt ttcttataag ccagaaatgt taaatggctc tttcgttcac
     1321 attgatgact ggagtggatg gctgatatta accaacaacc agtttgatga gtttaataac
     1381 attgcctctc agggtgacag cggttcagca ctgttcgtct atgataacca aaagaaaaag
     1441 tgggttgtcg ctggaactgt ctgggggatt tataattacg ccaatggcaa aaaccacgca
     1501 gcatacagta aatggaacca gacaaccatt gacaacctga agaacaagta ttcttacaac
     1561 gtggatatgt caggagctca ggttgcaacc attgaaaatg gaaaactgac aggcactggc
     1621 tcagacacca ccgatataaa aaataaggac ttaatattta ctggcggtgg agatatcctc
     1681 ctgaaatcct cttttgataa tggtgctggc ggtcttgtct ttaatgataa aaagacctat
     1741 cgagtaaacg gggatgattt cacctttaaa ggtgccggtg ttgatacaag aaacggcagc
     1801 accgttgagt ggaatatccg gtatgataat aaagacaacc ttcacaaaat tggtgatggc
     1861 acattagatg tccgaaaaac ccagaacacc aacctgaaaa caggtgaggg tcttgtcatt
     1921 cttggagctg aaaaaacatt caataatatc tacataacca gtggtgatgg aactgtccga
     1981 ctgaatgcag aaaatgcact gtctggcggt gaatacaacg gtattttctt tgcgaaaaat
     2041 ggcggaactc ttgacctgaa cggatataat cagtctttca ataaaattgc tgcaactgat
     2101 tcaggtgctg taataaccaa tacgtcaacc aaaaaatcca ttttatccct gaataatact
     2161 gctgactata tctatcacgg caacataaac gggaatctgg acgtacttca gcatcatgag
     2221 acgaaaaaag agaaccgtcg tcttattctt gatgggggcg tggacacaac aaatgatata
     2281 agcctgcgta atacacaact gtccatgcag ggacatgcca ctgaacatgc catttatcgg
     2341 gatggagctt tctcttgttc actaccagct cctatgcgct ttttgtgtgg cagtgattat
     2401 gttgcaggaa tgcaaaatac agaagctgat gctgtaaaac aaaacggaaa tgcctataaa
     2461 accaacaatg ctgtctctga tttatcgcag ccagactggg aaaccggaac attcagattt
     2521 ggaacgctac atcttgaaaa ttccgatttt tctgttggtc gtaatgcaaa tgtaatcggg
     2581 gacattcagg ccagtaaatc aaacattact attggtgaca ctacagcata tattgatttg
     2641 catgctggta aaaatattac cggtgatggt tttggcttcc gccagaatat tgtgcgtgga
     2701 aactcacaag gagaaacgct gtttacagga gggatcacag cagaagacag cactatcgtt
     2761 attaaagata aagcaaaagc attattttca aattatgtat acctgctgaa cacaaaagca
     2821 accatagaga acggtgctga tgtgacaact caaagtggta tgttctccac gagcgatatc
     2881 agcatctctg gtaatctgtc catgacaggc aatcccgaca aagacaataa attcgagccc
     2941 tcaatatatc tgaatgatgc ttcttatcta ctgactgacg actccgccag actcgttgcc
     3001 aaaaataaag catctgtggt gggagatata cactccacta aaagtgcatc catcatgttt
     3061 ggtcatgatg aaagcgacct ctcgcagttg tctgacagaa cctcaaaagg gcttgcactt
     3121 ggtcttttag gtggctttga tgtctcatat cgcggttcag tcaatgcccc gtcagcatct
     3181 gccactatga acaatacctg gtggcaacta accggagatt ctgcgctgaa aacactgaaa
     3241 agtacaaaca gcatggtcta tttcactgac agcgcaaaca ataagaaatt ccatacgctg
     3301 acggtcgatg agctggcaac cagcaacagc gcctatgcga tgcgtacaaa cctttctgaa
     3361 tcagacaaac tggaggtcaa aaaacacctg tctggtgaga acaatatttt actcgttgat
     3421 ttccttcaga aaccaacgcc tgaaaaacaa ctgaatattg aactggtaag cgcgccaaaa
     3481 gacaccaatg aaaatgtctt taaagccagc aaacaaacca ttggtttcag tgatgtaacg
     3541 ccggtcatta caaccaggga aaccgatgac aaaataacat ggtcactgac aggctataac
     3601 acggtagcaa acaaggaagc aacccggaat gccgccgccc tgttctctgt tgactataaa
     3661 gcgtttctga acgaggtcaa caacctgaac aaacgtatgg gtgacctgcg tgatatcaac
     3721 ggcgaagccg gtgcatgggc acgcatcatg agcggtaccg gctctgccag tggtggtttc
     3781 agtgacaact acacgcacgt tcaggtcggg gtcgacaaaa aacacgagct ggacggactg
     3841 gatttgttta ccggtttcac tgtcacacac actgacagca gtgcctccgc cgatgttttc
     3901 agtggtaaaa cgaagtctgt gggggctggc ctgtatgctt ccgccatgtt tgattccggt
     3961 gcctatatcg acctgattgg caagtatgtt caccatgata atgagtacac tgcaaccttt
     4021 gccggactcg gaacccgtga ttacagcacg cattcatggt atgccggtgc agaagcgggc
     4081 taccgctatc atgtcactga ggatgcctgg attgagccac aggctgagct ggtttacggt
     4141 tctgtatccg gtaaacagtt tgcatggaag gaccagggaa tgcatctgtc catgaaggac
     4201 aaggactaca atccgctgat tggccgaacg ggtgtggatg tgggtaaatc cttctctggt
     4261 aaggactgga aagtgatagc ccgtgccggt ctgggctacc agttcgacct gctggctaac
     4321 ggcgaaaccg tattgcggga tgcatctggt gaaaaacgca tcaaaggtga aaaggacagc
     4381 cgtatgctga tgtccgttgg cctgaatgca gaaatcaggg ataacgtccg ctttggactg
     4441 gagtttgaga aatccgcctt tggtaagtac aacgttgata atgcagtcaa cgctaatttc
     4501 cgttactcgt tctgatactc cgtattcaaa agggcctgcg ggccctttac gctcagtgcc
     4561 tgccgccctg atatgtctga gagacatttc cgggatgtca gtcgactggt ttacttaact
     4621 gcgatgacta aagctggcag taatgccggc ttttttacgg ccacatacgt cttcctctca
     4681 tccggaactg tgatacaaat cctgcataca ggattgaccg ccggttcctg caaatggcac
     4741 attttcagta agatagcgaa ggttacattc ccccgtcatg tgtggcacaa gctgtcaccg
     4801 ggaaagaaac ggaaagcaca tgaaaaacat caaaactcac accggactcc ttatccacaa
     4861 agaacaaacg cggcgcgttc gtcttcatga aacacccact gcctggtgcc acacgcaccg
     4921 ggaatgctac agcaaaacga ccggtcgccg ctgcggcagc cctgacagtc tcagcaggct
     4981 gattctgagc agt
//
LOCUS       DAZGJW010000244         4959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZGJW010000244 DAZGJW010000000
VERSION     DAZGJW010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.246"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(173..934)
                     /gene="kduD"
                     /locus_tag="ACHKIK_004841"
     CDS             complement(173..934)
                     /gene="kduD"
                     /locus_tag="ACHKIK_004841"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO9085844.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(964..1800)
                     /gene="kduI"
                     /locus_tag="ACHKIK_004842"
     CDS             complement(964..1800)
                     /gene="kduI"
                     /locus_tag="ACHKIK_004842"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO9085845.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(2087..3268)
                     /gene="yqeF"
                     /locus_tag="ACHKIK_004843"
     CDS             complement(2087..3268)
                     /gene="yqeF"
                     /locus_tag="ACHKIK_004843"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO9085846.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3523..4752
                     /gene="yqeG"
                     /locus_tag="ACHKIK_004844"
     CDS             3523..4752
                     /gene="yqeG"
                     /locus_tag="ACHKIK_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9085847.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1312 a         1252 c         1069 g         1326 t
ORIGIN      
        1 ttgaattatt gagattatta ttaaccacct aattttacag cagataaaat tcataaagtt
       61 cattaattga taattaatat ggattatttc ataaccatga tatggattat gatgatctac
      121 aggtataaaa aaccctgcca tgcggcaggg tcataaaagt aagaagaatg aattaacgcg
      181 ccagccaacc gccatccacg gcaatggtat aaccattcac ataatctgaa gcgctggagg
      241 caaggaacac tatcggcccc atcaggtcac tcggcagtcc ccaacgacca gctggaatgc
      301 ggtcgagaat ttccgcgcta cgttgttcat ctgcccgtag ttgttgagta ttgttggtcg
      361 ccatgtaacc cggagctatc gcattaacat taatgttgtg tttagcccat tcgttcgcca
      421 tcaatcgcgt cacacccatc acgccgcttt ttgatgcggt ataagaaggc acacggatcc
      481 cgccctggaa ggagagcatt gacgcgatat tgataatctt gccgccattg ccttgcgcga
      541 taaagtgttt cgccgctgcc tgagacatga agaatacgct cttgatattc aggttcatga
      601 catcgtccca gtccttttcg ctgaactcga gagcatcttc gcggcgaatc aatccggcgt
      661 tattcaccag gatatcaata tgaccaaact ccgctaccgc gcgatccagc agtccaggaa
      721 tgccatcaat ctttcgcaga tcggcggtca ggcttaaaaa acgacgcccc agcgccgtga
      781 cctgctcgat ggtttcagtc ggttcaacga tgttaatgcc aacaatgtca cagcccgctt
      841 gcgccagccc caacgccatc ccctggccca gcccagtatc acaaccagtg acgaccgcaa
      901 ctttaccttc gagagaaaat gcacttaaaa tcataacaat accttattcg tttatgccca
      961 caactagcgc aaatctttaa cggccacatg gtccatatca tcaaagacct ggttttcacc
     1021 gaccatgccc cagataaagg tataagcttt ggttccgacg ccggaatgaa tcgaccagct
     1081 cggggagatc accgcctgct cgttatgcat cacaatgtga cgcgtttctt gcggctgccc
     1141 catcatgtgg aaaacgcagg cgtcatcatc catattgaaa tagaaataaa cttccatccg
     1201 gcgctcgtga gtgtgacacg gcatggtgtt ccacaagtta cctggagcca gctccgtcag
     1261 ccccatactc aattggcagg tttccagcac atccgggaca aaatatttgt taattgtgcg
     1321 acggttactg gtgaggttat cgcctaacgt gactggagat acttcgtccg gtgtgacttt
     1381 tttggtggga tacgtcgtat gcgcgggtgc gcaattgtaa taaaactttg ccggagtggc
     1441 ggtatcaata ctggcaaaga caacttcttt tgcaccttta cccacataca gggcgtcgcg
     1501 gtgaccgatt tcatagcatt ggccatcgac agtaatcgta ccggcaccgc caatattgat
     1561 aacacctaac tcgcgacgtt caaggaaata gcttacgccg agttgtttac caacttcccc
     1621 gccaacggaa accgttttag ttatcggcat aatgccgcca acaataattc ggtcaatgtg
     1681 gctgtaaacc atggtgtact catcggcgac aaatacctct tcaaccaaaa attcattgcg
     1741 cagcccttgg gtatccagcg tttttgcgtg cgcactgtgg atgctctgtc ttacgtccac
     1801 atcaacctcc gataatcagt gaagcgtgaa aatagaaaac gatagtgaaa cactgtttcg
     1861 ttttctctat aaagggatga tatgcccatg ggctagggtt ttcaattaaa aataaaacaa
     1921 tgtttcattt ttaagttaag aattaaaaaa agtgcagtgt cgatcacgaa tatgtcatga
     1981 gaatggaaaa atgagggagc ccagaaaatg tatgtcgact tgccagccat cattattcaa
     2041 cagattaatg acggcagata tttatcggta aggaggaaat gcagagctac tcgtcacgtt
     2101 caatggtcaa tgccacgccc tggcccccgc cgatacaaag cgttgccagc cctttgcggg
     2161 cattacgttt caccatttca tgaaccagag aaaccaggat tcggcaaccg gaagcgccta
     2221 tcgggtgacc gagtgcgatc gcgccaccat tgacattgac ccgacgctca tcccactcaa
     2281 gcatcttgcc aaccgaaagc gcctgtgcag caaacgcttc attagcctcg ataagatcga
     2341 catcagccaa ctgccagcct acacgctcca ggcaacggcg ggtcgcatac accggcgcaa
     2401 ttcccatcaa tgccggatct acaccaacgc tggcaaatgc gcggatgcgg gccagcacgg
     2461 gtaaattcaa cgctcgtgct ttggcttcgc tcatcatcat taccgcagct gcgccatcgt
     2521 ttatggatga tgcattcccc gcggtcacag aaccgagact atcaaatgaa ggatttaaac
     2581 gggctaaacc ttctgcactg gcgtcagtgc gtggctgttc atcggtatca acaaccaacg
     2641 tctgaccgtt actttggatc attaccggga cgatctcatc tttaaatcgt ccggcgtcaa
     2701 tcgccgctcg cgctttttgt tgcgagctaa gtgcgtaagc atcctgcaac tgacggctga
     2761 tgccatattc gcgagccaga ttttcggcgg tgacaccaat atgataatca ttgaaggcat
     2821 cccacaaccc atcatgcacc agactgtcaa ccaactggct attgccaagc tgtgcaccgg
     2881 tgcggctatc agtcagaaca tgtggtgcgc ggctcatgtt ttcctggcca ccggcgatga
     2941 caatatcagc ctcgccacac tgtatcgcct gagtagccag atgcagtgct ttaagcccag
     3001 aaccgcaaac gtcattaata gtgattgcag aaacgctatt aggaagacca cctttaatag
     3061 ccgattgcct tgccggattc tgccctgccc ctgcagtcaa cacctgacca agaattactt
     3121 catccaccgc atatgcagga acgccggtac gttctattaa cgctttcacg accagactac
     3181 caagttccac ggcggaatga cccgctaacg caccgcgaaa gcagccgata ggtgtccgta
     3241 acgccccgac aatcacaacg tctttcatca caacctcgca ccaattaaca gtgcaatagt
     3301 aaatgattgt taattacagt tatcttaatt gtttaaaaaa agtgattttt atcacaaagg
     3361 aaatatgcct gagcagtagt cagagtcata actggcacgt aaggtttgca accactaacc
     3421 caccaataga ggggtagata gggcgttaat ctcccatact taacctggtt tatggtaaat
     3481 tgccctccat tttgtttaat ttgtagatga tacgttcaga taatgtctaa tatttggtct
     3541 aaagaagaaa ctctgtggag tttcgcgctc tacggcacag ccgttggtgc aggcacgctc
     3601 ttccttccta ttcagttagg ttcggcaggg gctgtggtcc tgtttattac tgctctggtc
     3661 gcctggcctt taacatattg gccacataaa gccttatgcc agttcatcct ctcatcgaaa
     3721 acatcagcag gtgaagggat aacgggcgca gtaacacact actatggcaa gaagattggt
     3781 aatctgatta ccacgctgta cttcatcgcc ttttttgtcg tcgtgttgat atatgcagtg
     3841 gcaattacca actcacttac ggaacagctg gcaaagcata tggttattga tcttcgcatc
     3901 cgtatgttgg tgagtctggg tgttgtatta atcctgaatc tcatttttct gatgggacgc
     3961 catgcaacca ttcgggtaat gggttttttg gtattcccat tgattgccta tttcttattt
     4021 ctttccattt accttgtcgg tagttggcaa cctgatctat taacgaccca ggtagagttc
     4081 aatcagaata cccttcacca gatatggata tcgattcccg tgatggtttt cgcctttagc
     4141 catacgccca ttatttctac gtttgccata gacagacgtg aaaaatatgg cgaacacgct
     4201 atggataaat gcaaaaaaat tatgaaagtc gcttatctca tcatctgcat aagtgtactg
     4261 ttctttgtct ttagctgcct gctttctatt ccaccttcgt atattgaagc ggctaaagaa
     4321 gaaggggtca ccattttatc ggcgctttct atgctgccga acgccccagc atggctgtca
     4381 atttccggga ttattgtcgc agtagttgcg atgtcgaaat cattcctggg tacgtacttt
     4441 ggcgttattg aaggtgccac ggaggtcgtc aaaacaacac tacagcaggt tggtgtaaag
     4501 aaaagtcgtg catttaaccg cgcactatca attatgttgg tatcgctgat caccttcatt
     4561 gtttgttgca ttaacccgaa cgcgatttcg atgatttacg cgatcagcgg cccgctcatt
     4621 gccatgatac ttttcatcat gcctacgttg tcaacgtatc tgatcccggc gcttaaaccc
     4681 tggcgttcca tcggaaatct gattacgctg atcgtgggta tcctgtgcgt atcggtaatg
     4741 ttctttagct gatatccatt gtgaatggtt caaaattgct acaattttga accattccag
     4801 ttttcataat gttcattcgt tattattccg cactatcact tccctcatca tatcctagta
     4861 ttatcagtta gttaaacaca caaagagatc tgaacatctt aaataagact atttaagatg
     4921 cataacttag attcgcaaga tatcttccgg tcattcaaa
//
LOCUS       DAZGJW010000245         4949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZGJW010000245 DAZGJW010000000
VERSION     DAZGJW010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.247"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..360)
                     /locus_tag="ACHKIK_004845"
     CDS             complement(<1..360)
                     /locus_tag="ACHKIK_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO9085848.1"
                     /translation="MTLTLLTAYNVPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIA"
     gene            complement(627..1304)
                     /locus_tag="ACHKIK_004846"
     CDS             complement(627..1304)
                     /locus_tag="ACHKIK_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFO9085849.1"
                     /translation="MTILSLENPVILLLENMLEALSTAPDSINNERRRRRYLLNWLES
                     ARQMSAFRGMAEEFTTLRKLLATNTDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGSGSGRNMLSRILHVGHSGLPKAEWGID
                     HSNHLLH"
     gene            complement(1445..2086)
                     /locus_tag="ACHKIK_004847"
     CDS             complement(1445..2086)
                     /locus_tag="ACHKIK_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO9085850.1"
                     /translation="MCLSELQAGLNEDQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNIRELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLNFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAPPLPESRIALLLHLVSDDGALT"
     gene            2781..3812
                     /gene="repA"
                     /locus_tag="ACHKIK_004848"
     CDS             2781..3812
                     /gene="repA"
                     /locus_tag="ACHKIK_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFO9085851.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATSFLVEPERK
                     RTRRRRGEHSTKPKCENPSWYRPARYKELSGQLGHAYNRLVKKAPVTGEQSLRMRMSR
                     HPFYVQKRTFVGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVTRLAKEISPKD
                     SKGNVIPELEVTVSRLSRLLAEQVRFGVLGVSEETMWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
BASE COUNT         1327 a         1212 c         1307 g         1103 t
ORIGIN      
        1 ggcaatcagt ctgccggtgc ggtgtacgag aggacatgtc agcagcaggc aaagtggtgc
       61 aaggagaatg ttcggggcag gagcctgcag cagatgaata ataagaccct gcaggagaat
      121 gccggatata ccaaaaaaac ctgccagcat acagaggacg atcagcaggg ggatccgtcc
      181 gatgttcagc cagtccagcg cctctcctgt cagcccctcc ggtaaatcag gtgtatccat
      241 agctgaagag agggagtgtc cgcagagcag ggcgataagt tcagcgatac cagtaagcag
      301 aacaaaagtc agggctgcca gatagggtac gttatacgcg gtaagtaacg tcagtgtcat
      361 ttttcctcct gtggcaggtc agtcaaacaa cattatggcg tgaagcttcc gcttctttaa
      421 ttgtcagaag aagcatatcg tcagtcatca gaagacaagg ggatgtctgc aggaggggat
      481 gataacaact gaagcctcac gccggaatgc gctttatgac aaccggagag gacggataat
      541 aaaaaatggg acatccacat cagcaaaccg cgatttgcga aaaaaagtca gcggagagtg
      601 gctgccgttg tggctgtccc ggtatttcag tgtaacaggt gattgctgtg atctatgccc
      661 cattcggctt tcggtaaacc tgaatgcccc acatgaagaa tgcgtgacag catgtttctg
      721 ccgctgccgc tgccgctgcc gttcaggctg cattcgcgtc cgtgcgcgag tataaaacct
      781 tcatcccgga atatctcttc cacttctgaa gattcgaggg atgccggatg aattaatgaa
      841 aatgccaccg gtctgagcat tctgcccgtg gtggaaaagg tgtctctggt actgttcagc
      901 tgcaggatat gacgtttacc tgtttgcgca cgggaaagtg tctcactcag cagctggtcg
      961 ttccatgcgc acgtgccaat ctgccatccc agacttctgc acgcggaaag agccgagcgg
     1021 aagcggtaca ggtcgtataa tgctgcgtta ttgccacttt ccagcagtga tttcagcgta
     1081 tttgctaccg gtatgtccgt attggtggcc agcagttttc gcagagtcgt aaactcttct
     1141 gccattcctc tgaatgcgct catctgacgc gcagattcaa gccagttcag caggtaacgc
     1201 cggcgacgcc gttcgttgtt gatactgtcc ggggcggtgg agagtgcttc gagcatattc
     1261 tccagtaaca gaatgacagg gttttccagg gatagaatgg tcataaaggc gtcctgaaaa
     1321 aatgcccgac agccgctctc cgtacaccgt aaggtgtcac cggtgccggg cattgtggaa
     1381 acagaaggaa aaaaaatccc ggccataaag gccgggaaaa gtcgtcaaat taagacagca
     1441 attttcatgt caacgctccg tcatcagata ccaggtgcag gagcagggcg atcctcgact
     1501 ccggcagagg cggtgcatca agccggagga ggtactggta acgcgctttt gtcagccaga
     1561 gggtggaggc agcatccagc tcctccctgg ctgccggatt gagcgcagga tccgggtaca
     1621 ctttcatcaa agactgaacg atatcctccc tgacggtttt gatttcaccg gccattttca
     1681 cgaagttcag aaaggcactg tgacggcgaa ggtgagtgaa gctgtggcgt tcggggtctg
     1741 ctttcaggaa aaagtaaccg ggaaacacgg cgtgtatatg tcgacggtaa ccgcagactt
     1801 tatccgtgcg gcggactttt ttcagtatca ggggtgtcca gggaaggacg tgttgttcgg
     1861 aaagccagga aaagagatgt tcgcggtttt tgccggcagg gatgtactga gccagatacc
     1921 agttgcgttg atgtaattct ctgatattca aaatccggaa cgctccgcga aaatcgatcc
     1981 ccgcagaggg gcttcgatgt tgttgtgaac ggcgaataca taaagcatgt gtcggaaaca
     2041 gcggctgatc ctcgttcagc ccggcctgca gttctgacag gcacatgatt tacgcactct
     2101 tgtaagcctt gtggacggtt tcgaggccgg cgaaaaccga caaaacctgc aaacaggatt
     2161 aatggctggc aaacatatta cataaggata tatctgtggc aagagcgaag ataagtagtt
     2221 aaatagatcg ttatatttga taaagcagca tattacgctg ggttgtgtga ggtgtttgga
     2281 tgattagatc gtaattacaa gatcgattca tccaaaaaac atgaaaaaca cagcataaaa
     2341 gatcgtttgt ggaattgtct attttttgac acagttcaaa aaaacgacgt tttttaggtg
     2401 gttaatgaga ggcctggtaa atcagagaaa tcgtgttggc ttttcaaagc ggtggaaaag
     2461 gggtatattg cggatcgtta tgcagtggct tttgggatcg tcaggatccg ggaagtcaga
     2521 aaatggcagg atgcgccata aggcattcag gacgtatggc agaaacgacg gcagtttgcc
     2581 ggtgccggaa ggctgaaaaa agtttcagaa ggccataaaa ggaaaacccc cataatcttc
     2641 tcatcgaact tggcggaacg ttagaagatt atgggggcct cacagaatac gggataagta
     2701 tatatgaaac cgtgtcagag attcaaccct tcgcagtgta ttaacgcacg gcattctgtt
     2761 tccgccataa gcgacagctt gtggcaggtc tgaagaatac tccatataac gcagtacact
     2821 ggagtcagtt agcacccgaa gagcagatcc gtttctggga agactatgaa gcgggaaggg
     2881 cgacctcttt cctggttgaa ccggaaagga agcgcaccag gcgccgtcgt ggcgaacact
     2941 ccactaaacc caaatgcgaa aatccgtcct ggtatcgtcc tgcgcgctat aaggagctga
     3001 gcgggcagct cgggcacgcc tacaaccgtc tggtgaaaaa ggccccggtg accggcgagc
     3061 agagcctgcg tatgcgtatg tcgcggcatc ctttttacgt gcagaaacgg acgttcgtcg
     3121 gtcgcaaata tgctttccgg ccggaaaaac aacgcctcct cgatgccatc tggccggttc
     3181 tggtcagctt cagtgatgcc ggcacacaca ctgtggggat gagtgtcact cgcctggcta
     3241 aagaaatcag tccgaaagac agtaagggaa atgttattcc ggaactggaa gtgacggtct
     3301 cccgtctttc ccgtctgctg gccgaacagg tgcgttttgg tgtgctgggt gtttcagagg
     3361 aaacgatgtg ggaccgtgaa acccgccagc gtctgccgcg ctatgtctgg ataacaccgg
     3421 ctggctggca gatgctgggc gtcgacatgg taaaacttca cgaacagcag cagaaacgac
     3481 tgcgtgaaag tgaaatccgc cagcagctca ttcgggaagg tgttctgcgt gaggatgaag
     3541 atatctccgt acatgcggcc agaaaacgct ggtatctgca gcgcagtcag gatgcgctga
     3601 aacaccgtcg tgcgaaagca gcagccagta agcgcgccag acgcctgaag aaactgcctg
     3661 ccgaccagca gattcatgag atggcagagt atctcaggaa gcgtctgccg ccggatgaag
     3721 cctatttttg ttccgatgac catctgaagc gactggccat cagggagttg cgtcagcttg
     3781 aactgacgct ggctgccccg ccaccgcact agacagcacc attccctcag cactgaatca
     3841 tcgccagccc ctccggggct ttcggcgcag gtatcgctca gcccaaaatc cgcagtaatc
     3901 accttaaatc ccctcagagg ggcatatttg ctcataaaac cacgcatcag tcatcagaac
     3961 gtggccacgt tgtttcagtt atccacataa atccgcaaac aaagaacttt aagaagctgc
     4021 aaacctgaaa cagcaaacct gcaatatagt cttaacccca ttatttaatc ccctgcgttg
     4081 cttcgccgca gcgaaagtct ttatctctga aaccactgtg aacaaacaca aagaggcctt
     4141 cgcttgcagc ggccaaggcc gcgccgctca gaatctaaaa gcacctccca cgctgacgcg
     4201 cgggccccga acctcaccgt tctggaacca caacaaaaaa acatcaggaa taaaaacacc
     4261 acacaaacgc agcaccgtgc ccacccctca taactgaaaa gcgccgccgc cccggcccaa
     4321 agggccggaa cagagtcgct tttaattatg aatgttgtaa ctaaacatca tcatcgcttt
     4381 aagaccccat ttgtctagca gggcgatggc ccactgcgtg aaccatcacc ctgcacacag
     4441 ggtattttat gtgcaattgt caaccactga atcggaaccc cagaggggcc cccacgattc
     4501 agaagttgac aatttaagca cagggactac atgagaaaaa tcgtggttcg tctgtcatac
     4561 acgcgcgtaa gcggcactga tagccctctt acgcggaaat acgcccataa gcgctaactt
     4621 aagggttgaa ccgacgccac ggtgtctcgt taagacttca attgcgtttt gtaggcagtc
     4681 agggatactg tcccatgaat ggccacctgt aagctccaga taaccatttt tgttattctc
     4741 cacaacgaat tccgcatacc ggaaatcagc gctacttctg gggaggaatg gccttaagag
     4801 tgataaagat tatatgcttt tcatagagag actcatctat gccggtaaca ctgaaaaatg
     4861 tattgtgttc caagaagttt ttttgagtga acatattttc gctcacaaca atcgaattat
     4921 tcaaagccac aattttaaaa aatatttta
//
LOCUS       DAZGJW010000246         4932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZGJW010000246 DAZGJW010000000
VERSION     DAZGJW010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.248"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..965
                     /locus_tag="ACHKIK_004849"
     CDS             <1..965
                     /locus_tag="ACHKIK_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9085852.1"
                     /translation="LGRDRRIAITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWL
                     DRYEKILASRGIKQKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLSGYIDEGKAA
                     SAKLIRSTLSDAFREAIAEGHITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSP
                     CWLRLAMELAVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGIS
                     MKETLDKCKEILGGETIIASTHREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRS
                     LSARLYEKQISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
     gene            1100..2383
                     /locus_tag="ACHKIK_004850"
     CDS             1100..2383
                     /locus_tag="ACHKIK_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO9085853.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(2617..4878)
                     /locus_tag="ACHKIK_004851"
     CDS             complement(2617..4878)
                     /locus_tag="ACHKIK_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO9085854.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
BASE COUNT         1271 a         1261 c         1216 g         1184 t
ORIGIN      
        1 gattaggccg agacaggagg atagcaatca ctgaagcaat acaggccaac attgagttat
       61 tttcaggaca caaacacaag cctctgacag cgagaatcaa cagtgataat tctgttacgt
      121 tacattcatg gcttgatcgc tacgaaaaaa tcctcgccag cagaggaatc aagcagaaga
      181 ctctcataaa ttacatgagc aaaattaaag caataaggag ggggctgcct gatgctccac
      241 ttgaagacat caccacaaaa gaaattgcgg caatgctcag tggatacata gacgagggca
      301 aggcggcgtc agccaagtta atcagatcaa cactgagcga tgcattccga gaggcaatag
      361 ctgaaggcca tataacaaca aacccggtcg ctgccactcg cgcagcaaaa tcagaggtaa
      421 ggagatcaag acttacggct gacgaatacc tgaaaattta tcaagcagca gaatcatcac
      481 catgttggct aagacttgca atggagctgg ctgttgttac cgggcagcga gttggtgatt
      541 tatgcgaaat gaagtggtct gatatcgtag atggatatct ttatgtcgag caaagcaaaa
      601 caggcgtaaa aattgccatc cctacaacat tgcatgttga tgctctcggg atatcaatga
      661 aggaaacact tgataaatgc aaagagattc ttggcggaga aaccataatt gcatctactc
      721 atcgtgaacc gctttcatcc ggcacagtat caaggtattt tatgcgcgca cgaaaagcat
      781 caggtctttc cttcgaaggg gatccgccga cctttcacga gttgcgcagt ttgtctgcaa
      841 gactctatga gaagcagata agcgataagt ttgctcaaca tcttctcggg cataagtcgg
      901 acaccatggc atcacagtat cgtgatgaca gaggcaggga gtgggacaaa attgaaatca
      961 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
     1021 cttttttata atgccaactt agtataaaaa agcaggcttc aacggattca tttttctatt
     1081 tcatagcccg gagcaacctg tgaacacatt ttcagtttcc cgtctggcgc tggcattggc
     1141 ttttggcgtg acgctgaccg cctgtagctc aaccccgccc gatcaacgtc cttctgatca
     1201 aaccgcgcct ggtacctctt ctcgcccgat tctgtcggca aaagaagcgc agaatttcga
     1261 tgctcaacac tattttgcat ccctgacacc aggtgcagca gcgtggaatc cttccccgat
     1321 taccctgcct gcgcaacctg actttgttgt cggcccggcg ggcactcaag gtgtaacgca
     1381 taccacgatt caggcggcgg tagatgcggc aattatcaag cgtaccaaca agcgccagta
     1441 tattgccgtg atgcctggtg agtatcaggg aacggtatat gtccctgccg ctccgggtgg
     1501 aattactctg tacggtacag gtgaaaaacc gattgatgtg aagattgggc tttcccttga
     1561 tggtggcatg agccctgccg actggcgtca cgacgtcaac ccgcgcggca aatatatgcc
     1621 aggtaaacca gcgtggtata tgtacgatag ctgccagagc aaacgcagcg acagtatcgg
     1681 tgttctctgc tctgcggtct tctggtcaca aaacaatggc ctgcaactgc aaaatctgac
     1741 catcgaaaac acgctgggcg atagcgtaga tgcaggtaac catccggcgg tggcactgcg
     1801 tactgatggt gaccaggtac agattaacaa cgttaacatt ctcggtcgtc agaacacctt
     1861 ctttgtcacc aacagcggtg tgcagaaccg tctggaaacg aatcgtcagc cgcgtacgct
     1921 ggtgaccaac agctacattg aaggggatgt ggatatcgtt tctggtcgcg gcgcagtggt
     1981 gttcgataac accgaattcc gcgtggtgaa ctcacgtact cagcaagaag cgtatgtgtt
     2041 tgcaccggct acgctgtcca acatttacta cggtttcctc gccgtaaaca gccgtttcaa
     2101 tgctttcggt gatggtgtgg cgcaactggg ccgctcgctg gatgttgatg ccaataccaa
     2161 cggtcaggtg gtgatccgtg atagcgccat caacgaaggt tttaacacgg ctaaaccgtg
     2221 ggccgatgcg gtgatctcta atcgtccgtt tgcgggtaat accggcagcg tagatgataa
     2281 cgacgaaata cagcgcaatc tgaatgacac taactacaac cgcatgtggg aatacaataa
     2341 ccgcggcgtg ggtagtaaag tggttgcaga ggcgaagaag taagagcaat taactatttg
     2401 ccggatgcgg cgtaaacgcc ttatccggcc tacggttcga tgcgatttgt aggtcggata
     2461 agatgcgcaa gcatcgcatc cgacaataag tgccggatgc tgcgaaaatg ccttatctgg
     2521 cctacagatt cgatgcgatt cgtaggtcgg ataagatgcg caagcatcgc atccgacaat
     2581 aagtgccgaa tgcgacctac attcacatgg cgctttttac atctgacggt ttttattgaa
     2641 gttaatcaaa ctacccgcct tgataatctc gcgctcttca gcagtcagac tttccatata
     2701 gagcgtaatt tccgttaccg gcgcatcttc atggatcaca taacctttaa acgtcgtacc
     2761 cggattatcc agcgccgctt taatgccagg gatgtaaatg taatccccca cttcaaaggt
     2821 tggcacttcc gccatctgca gcggtaacat cccccagttg atgacgttag aacggtagcg
     2881 tttagtcgcg tactcttcgg caatattcgc cagaccgcca atcacacgct ggcagctcgc
     2941 cgcctgttca cgcgcagaac catcgcctgg tttcaccgca tataccatgc tgccaatttc
     3001 agtttgcagc ggatcaatat gctcctgacc agcaatctgc ttaatgcgcg caaacacctc
     3061 tgtcagctcg ctgacattcc ccgccagacg ctgattttcc agctcagcag tcgctttact
     3121 tctgccaaca taaccgggat cgcggcgtga cagggtaaac tccgccagac caatcggatt
     3181 tgaacgataa gaagaggttt caccggaagg aatcagttcg tcggtggtgg tcacttcgtc
     3241 gaggatcttc gagcacactt tcaggacgat attgtcagtc agcgcaccca attccggcca
     3301 gtctttaatg ttcggtccgt aaatcagcgg ttgctgagtt gcccctttca caaagccctg
     3361 ataaacacgg tttttatacg gcgttacatc gaaggcgtac tccggcacgt tgtcccagca
     3421 atcaagttcg ctggcagagg ttaaatagcc accgtttgcc gcagtcgcag cgatagaacg
     3481 agcgtccatc aacgccaccg ctgacatctg cccattagct ggcttagagc cttcgcggtt
     3541 cgggaagtta cgcgtggtgt ggcgaatact caaaccgttg ttgattggcg tatcgcccgc
     3601 gccaaagcat gggccgcaga acgcggttct gatgattgcg cctgcgccaa tcaaatctgc
     3661 taccacacct tttttggcga gatccataaa caccggctgt gatgacgggt aaactgccag
     3721 cgagaaggtg tcattgccac aggattgacc gcgcagtgca ttcgccgccg cgatgacgtt
     3781 ttcgtagtta ccgccagaac agcccgcgat aatcccctgc tgcactttca ggcgaccatt
     3841 ttccacttta tccagcagcg agagtttggc tttaccgtgc gccacgcgtt cggactcaat
     3901 ttcaatctca cgcagaatgt cggtcaggtt ctggttcagt gtgtcgattt catacacgtt
     3961 gctcgggtgg aacggcagcg caatcattgg tttgatggcg cttaaatcaa cgctgatgca
     4021 gccatcgtag tacgccatcg gttgagggtt aagctggcag taatcctggc cgcgaccgtg
     4081 cagcgccagc cagttatgca cttcttcatc agtttgccag acggagctta aacaggtggt
     4141 ttccgtggtc atcacgtcaa cgctgttacg gaaatcggta gagagcgcgc taacgcccgg
     4201 tccaacgaac tccatgactt tgtttttgac gtaaccgttt ttgaacaccg cgccaatgat
     4261 agccagcgcc acatcctgtg gccccacata cggcgcaggt tttccggtca gatgcaccgc
     4321 aaccacgccc ggatagtcga tatcccaggt gtcattaagc agctgtttta ccaactcacc
     4381 gccgccctca ccgactgcca ttgtccctaa tgcaccgtaa cgggtgtggc tgtctgaccc
     4441 gaggatcatt ttgccgccgc ctgccatcat ctcacgcata tattgatgga tgaccgcaat
     4501 atgcggaggc acaaaaatac cgccataacg ctgggccgcc gataaaccaa aaacatggtc
     4561 atcaccgtta atagtgccgc cgacggcgca gagtgagtta tggcagttgg tcagcacata
     4621 cggcagcggg aaacgttcca taccggacgc tttagcggtc tgtacaatac caacaaaggt
     4681 aatatcgtga gaggctaatg aatcaaactt aattttaagt ttatccatat ttccggacgt
     4741 attatgcgaa gagagaatag accaggcaat agtgcctttt ttggcttctt cttttttaat
     4801 ttcgccggtg aaatgttctt cggcaattat ttcgttatta ctggcgagaa acacgccttt
     4861 ttcagataac ttgatcatca aatgctccag atatctgcgc accgtgaagt gcgcagaaag
     4921 ataataaatg ga
//
LOCUS       DAZGJW010000247         4923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZGJW010000247 DAZGJW010000000
VERSION     DAZGJW010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.249"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            185..1159
                     /gene="hemB"
                     /locus_tag="ACHKIK_004852"
     CDS             185..1159
                     /gene="hemB"
                     /locus_tag="ACHKIK_004852"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO9085855.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1265..2116)
                     /gene="tauD"
                     /locus_tag="ACHKIK_004853"
     CDS             complement(1265..2116)
                     /gene="tauD"
                     /locus_tag="ACHKIK_004853"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO9085856.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTIAPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2113..2940)
                     /gene="tauC"
                     /locus_tag="ACHKIK_004854"
     CDS             complement(2113..2940)
                     /gene="tauC"
                     /locus_tag="ACHKIK_004854"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO9085857.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2937..3704)
                     /gene="tauB"
                     /locus_tag="ACHKIK_004855"
     CDS             complement(2937..3704)
                     /gene="tauB"
                     /locus_tag="ACHKIK_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO9085858.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3717..4679)
                     /gene="tauA"
                     /locus_tag="ACHKIK_004856"
     CDS             complement(3717..4679)
                     /gene="tauA"
                     /locus_tag="ACHKIK_004856"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9085859.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1148 a         1412 c         1247 g         1116 t
ORIGIN      
        1 aagagctata aataaagatt aaaaatattt tcagtgaata tgaaattatt gctatttcta
       61 ttacaaagat gtcctggtct ctctttcatt ctcttcttgt caacatcacg acaactttcg
      121 taaaacactc ctgccccgct tcaggtatac tgtgcacctt aattccacaa acatcaggca
      181 gaccatgaca gacttaatcc aacgccctcg tcgcctgcgc aaatctcctg cgctgcgcgc
      241 tatgtttgaa gagacaacac ttagccttaa cgacctggtg ttgccgatct ttgttgaaga
      301 agaaattgac gactacaaag ccgttgaagc catgccaggc gtgatgcgca ttccagagaa
      361 acatctggca cgcgaaattg aacgcatcgc caacgccggt atccgttccg taatgacttt
      421 tggcatctct caccataccg atgaaaccgg cagcgacgcc tggcgggaag atggactggt
      481 ggcgcgtatg tcgcgcatct gcaagcagac tgtgccagaa atgatcgtca tgtcagacac
      541 ttgtttctgt gaatacactt ctcacggtca ctgcggtgtg ctgtgcgagc atggcgtcga
      601 caacgacgca actctggaaa atttaggcaa gcaagccgtg gttgcagctg ctgcaggcgc
      661 agacttcatc gccccttccg ccgcgatgga tggccaggta caggctattc gccaggcgct
      721 ggacgctgca ggctttaaag atacggcgat tatgtcgtat tcgaccaagt tcgcctcttc
      781 cttttatggt ccgttccgtg aagctgccgg aagcgcatta aaaggcgacc gcaaaagcta
      841 tcagatgaac ccaatgaacc gtcgcgaggc gattcgtgaa tcactgctgg atgaagccca
      901 gggcgcagac tgcctgatgg ttaaacctgc tggagcatac ctcgacatcg tgcgtgagct
      961 gcgtgaacgt actgaattgc cgattggcgc gtatcaggtg agcggtgagt acgcgatgat
     1021 taagttcgcc gcgctggcgg gtgctataga tgaagagaaa gtcgtgctcg aaagcttagg
     1081 ttcgattaag cgtgcgggtg cggatctgat tttcagctac tttgcgctgg atttggctga
     1141 gaagaagatt ctgcgttaat ttttttcctc tcagaaaggg ggagttaacc tgttgaaagt
     1201 acggtgaacg ccccctcacc ctaaccctct ccccaagggg gcgagggaac cgtccgcact
     1261 cgtatcaccc cgcccgataa aacggtttat ccccaaggat cgtcgcccga tgcataatcc
     1321 gccgctgtgg caggtaatcg gcattggcat agtgctgagt cacgcggtta tcccaaatcg
     1381 caatatcatt cggttgccag cgccaacgca cctgaaactc cggtttagtg atatgggcga
     1441 ataaaaagcc taacaaggct tcgctctctt tctcgctgac atcgacaatt cgcgtagtaa
     1501 agccttcatt cacaaacagc gcctgtttac cgctcaccgg atgcgttcgc accaccggat
     1561 gtagcaacgg aggatttttc gcgaccgcct cgcgccagcg ttgatgttcc tcctcggttt
     1621 tgcggtattt gtattccggg aacgatttac ggaaatcatg ctccgcacgc agcccgctca
     1681 gcagctggcg gaagggaaca gagagcgcct cataggccgc aataccgctg gtccagagcg
     1741 tatcaccgcc ggtcgaaggt aactctttag ctgccagaat cgcccctgcg ggtggcgttt
     1801 caataaatgt cacatcggta tgccagttgt cgttatccgg cggattatcg ttatgggtat
     1861 ccagcacgat gatctcgtca accccttcgg catgagggta aacagggtga atatgcagtt
     1921 cgccaaaacg ctgggccagc gcgcgttgct gctgcggcgc aatagtttga tcgcgcagaa
     1981 acaccacctg atggcgcagc accgcatggt aaagctgttc aaactgatta tcgcttaacg
     2041 ggcgcgtcag gtcggcaccc gaaatttgtg cgccgatata cggccccagc ggggtaatgc
     2101 tcagacgttc actcattgta cttctccatg ccagggcgtc agacggcgct gtaacgcgcg
     2161 caaacccagt tctaaaagaa aggcgataat cgcaatcacc gcgatccccg ccagcaccac
     2221 gtcagtcgcg agaaattcac ccgctgactg aaccataaat cctaaaccgc gcgtcgcggc
     2281 aatcagctcc gccgctacca gcgtagacca gcccacaccc agaccaatgc gtaatccggt
     2341 gaggatttcc ggcagcgcgc cgggcaaaat gacaaaccac agcacctgcg cacggctggc
     2401 acccagcgac tgggcggcac gaatgcgaac ctgctgcgcg cttttcaccc ccgccagcgc
     2461 cgacatcgcc accggtgcaa aaatcgctaa atagatcagt aagatcttcg aggtttcacc
     2521 aataccaaac cagatcacca tcagcggcaa ataagccagc ggcggcaccg gacgataaag
     2581 ttcgattacc ggatccagaa tgccgcgcac cgtcgggctt agccccatgg cgatcccgac
     2641 cggaataccg atcaccaccg ccgcgagtag cgccagcaca atgcgcgtca ggctggctgc
     2701 cagatgctgc cacagcgtgg cgtccataaa tccctgcggt ccggcaatgg tgagcagttt
     2761 tgccagtact tgttgcggcg gcggcaaaaa cagcgggcta atcagttgca gcgccgccac
     2821 cgtccaccat acagtgagta aaaccgctaa cgtgccaatg cttaaggtca cctgacgcga
     2881 gagcggccag cgccatttca gccgccgcga atgcagtttt tcattaatga gcacactcat
     2941 gagaacgcct cccgttgctc aaatacgcgg cttaaaacat attcgcgcat ggcgatgaat
     3001 tgtggatcgg acttgatgct gcggctcgac tctcccgcaa caaagcggcg agcaaagttg
     3061 agcggcagcc gctccagcac gcggccaggg ccgggtgaaa gcagaaccag ttcagtcgcc
     3121 ataaacaccg cttcttctat atcgtgggta atcaacagca cctgcttgcc cgtctcctgc
     3181 cagagtttca gcagcagggt ttgcatctgg tcgcgggtga aggcgtccag cgcaccaaac
     3241 ggctcgtcga gtaataacag ctggggattc gccgccagcg cgcgggcaat ccccacccgc
     3301 tgacgttgac caccggaaag ctgccagata tagcgttttt ctgcgccttc cagccccact
     3361 tttttcagca tctggtgcgc gatttccagt cgctgcattt tctctatacc tgccaattgc
     3421 aggccgaacg ccacgttgtc ctgtacattg cgccacggta gcagcccttc attctgaaaa
     3481 accaccccgc gctctgctcc cggtccctca atacgcttac ccgccagttg aatgctgcca
     3541 tgctgataag gcacaaaacc ggcaatcaga ttcagcaggg tggttttacc gcagccggac
     3601 ggccccagca ccaccagtag ctcgccgctt tccagcgtca ggttgatatc ttccagtgcc
     3661 ggtttgccgc cataatcggc gtaaagatga gagatttgca gcatctgcgc ctccttttat
     3721 tgcacgaagc gcgaggtaac gtactggctg taatcattcg ctacagccgg gaccttgccc
     3781 tgctctttca aaaactgcgc ggtgtcgatg atcgctttgt tcaccggtcc ggtcagttct
     3841 gccgtttgtt gctgcggcgt cagataggta ttccccttca ccagccccgg aacgtcacct
     3901 tccggcacgc cgcttaaacg cgccagtttg ctgatgtttt ccggctgttt cagccacgcg
     3961 tctgggttag caatgtacgg ttgctgagca tcgatggcgc ttttagcgaa cgctttcacg
     4021 acctcaggat gtttctcggc aaaatctttg cgcaccaccc agacgtccag cgttggcgca
     4081 ccccactgcc cgacctgttc agaatcggtc agcaccttgc cgtctttttc cagggcgtta
     4141 accgccggtg cccagacata agcaccatca atatctcccc gctgccaggc agcgataatc
     4201 gcgggcggct gcaggttcac aatctccact tgcccgggtt taatgcccca gtgtttcagc
     4261 gccgccagca ggctgtagtg ggtggtagag ataaacggta cggcgatgcg tttgccaatc
     4321 agatcttccg gtttgctgat agttttcttt accaccagcg cttcggagtt acccagtttt
     4381 gacgccagca agaaaacttc aatcggcacc tgttggctgg ctgcaaccgc taacgggctg
     4441 gaaccgaggt tgccgatttg cacatcgcca gaagccagcg cccgcacgat gctggctccg
     4501 ctgtcaaact tacgccagtc cacggttgct ccgctttctt tagcaaaggt gttgtcggcc
     4561 tgagccactt tcgccggttc ggctgaggtt tgatacgcaa cggtgacgtt caccgcctgt
     4621 gcctgaaaag cgatgaatgc cagtgcggca agaagtgtgt tacgcgatga aattgccatg
     4681 attgtctgct cccctgtctt gttatgtgag cagtattcag gaataaaaac attcattaaa
     4741 agaattagtc gttatcgcac agatgatttt attcttagca aaaaaacggc aaaggaaaga
     4801 ttaaatatgc aattactccg ttctaacaat tgggaaaatt actacacttc attttagtga
     4861 tgcataaaca accacattac gagatttatt acttcattat ttctatcaat atcaatacat
     4921 ata
//
LOCUS       DAZGJW010000248         4817 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZGJW010000248 DAZGJW010000000
VERSION     DAZGJW010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.250"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..926
                     /gene="ybeF"
                     /locus_tag="ACHKIK_004857"
     CDS             <1..926
                     /gene="ybeF"
                     /locus_tag="ACHKIK_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO9085860.1"
                     /translation="LSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLN
                     LTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDIEGS
                     YDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTDLIID
                     NMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPEEQNFSGLR
                     QRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLN
                     EEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1135..2100
                     /gene="lipA"
                     /locus_tag="ACHKIK_004858"
     CDS             1135..2100
                     /gene="lipA"
                     /locus_tag="ACHKIK_004858"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO9085861.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2201..2404)
                     /gene="tatE"
                     /locus_tag="ACHKIK_004859"
     CDS             complement(2201..2404)
                     /gene="tatE"
                     /locus_tag="ACHKIK_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO9085862.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2533..3321)
                     /locus_tag="ACHKIK_004860"
     CDS             complement(2533..3321)
                     /locus_tag="ACHKIK_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO9085863.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3414..3797
                     /gene="crcB"
                     /locus_tag="ACHKIK_004861"
     CDS             3414..3797
                     /gene="crcB"
                     /locus_tag="ACHKIK_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO9085864.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3852..4061)
                     /gene="cspE"
                     /locus_tag="ACHKIK_004862"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3852..4061)
                     /gene="cspE"
                     /locus_tag="ACHKIK_004862"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO9085865.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4236..4796)
                     /gene="pagP"
                     /locus_tag="ACHKIK_004863"
     CDS             complement(4236..4796)
                     /gene="pagP"
                     /locus_tag="ACHKIK_004863"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO9085866.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1272 a         1221 c         1086 g         1238 t
ORIGIN      
        1 gtttaagcag aaagtcgtca gaaggtaaac cacaaatatt tacaacctta cgaaatattg
       61 atcttaacct tctgactatt tttgaagctg tatatgtaca taaagggatc gttaatgcag
      121 cgaaagtgct taatctgacc ccctcggcaa tcagtcagtc tattcagaaa ctgcgcgtta
      181 tattccctga cccattgttt attcgcaaag gccagggtgt cactcctacc gcatttgcga
      241 tgcatctaca tgagtatatc agtcagggcc ttgagtccat tcttggcgcg ctggatatcg
      301 aaggaagcta tgataagcaa cgaacgataa ctattgcgac cactccctcg gtcggagccc
      361 tggtccttcc tgtcatctat cgggcgatta aaactcacta tccgcagctt ttactgcgca
      421 acccgcccat cagcgacgcg gaaaaccaac tcagtcagtt tcaaaccgat ctcatcatcg
      481 ataacatgtt ttgcaccaat cgtacggtgc aacatcatgt tctgttcacc gacaatatgg
      541 tgttaatttg ccgcgaggga aatccactac tctctttaga agatgacaga gagactatcg
      601 acaacgctgc gcatgtactt ctgttaccgg aagagcaaaa tttcagcggt ctgcggcaga
      661 gagttcaaga aatgtttccg gaccggcaaa tcaatttcac cagctacaac attttgacaa
      721 tcgctgcact ggttgccaac agtgacatgt tagcgattat tcccagccgt ttttataacc
      781 tgtttagccg ctgctggccg ctggaaaaac tgccttttcc gtccttaaat gaggagcaaa
      841 tagatttctc tatccactac aacaaattca gcctgcgtga tcccatcctg cacggagtaa
      901 tagatgttat ccgtaatgca ttttgaaaaa gtattgttac agccagatca cagcgcagaa
      961 cgataaaatg gcacaatcca acaacaattt tacattttcg cgaccgcttt ggctgctttc
     1021 aggtccgttt caatgatata ctgccagtcg ttaattcaaa aatagttgat aattacaaca
     1081 atctattgaa ttgaaacgct ttccttcgta attcgcaact ggaacacgca cgctatgagt
     1141 aaacccattg tgatggaacg cggtgttaaa taccgcgatg ccgataagat ggcccttatc
     1201 ccggttaaaa acgtggcaac agagcgcgaa gccctgctgc gcaagccgga atggatgaaa
     1261 atcaagcttc cagcggactc tacacgtatc cagggcatca aagccgcaat gcgcaaaaat
     1321 ggcctgcatt ctgtctgcga ggaagcctcc tgccctaacc tggcggaatg cttcaaccac
     1381 ggcacagcaa cgtttatgat cctcggcgct atttgtaccc gccgttgtcc gttctgtgat
     1441 gttgcccacg gtcgcccggt agctcctgat gccaatgaac cagtgaaact ggcgcagacc
     1501 attgccgata tggcgctgcg ttatgtggtt atcacctccg ttgaccgtga tgacctgcgc
     1561 gatggcggtg cccagcactt tgcggattgc attactgcca ttcgggaaaa aagcccgcaa
     1621 atcaaaattg aaactctggt gccggatttc cgtggtcgta tggatcgtgc tctggatatt
     1681 ctgactgcaa cgccaccaga tgtgttcaac cataacctgg aaaacgtacc gcgtatttac
     1741 cgtcaggtac ggcctggtgc agattacaac tggtcgctga agctgctgga acgctttaaa
     1801 gaagcgcatc cggaaatccc gaccaagtct ggtctgatgg tgggactggg tgaaaccaat
     1861 gaagaaatta ttgaggtaat gcgcgacctg cgccgtcatg gtgtgacgat gttaacgctg
     1921 gggcaatatt tgcagccaag ccgccatcac ctgccggttc aacgttacgt tagcccggat
     1981 gagttcgacg aaatgaaagc cgaagcgctg gcgatgggct ttacccatgc tgcatgcggt
     2041 ccgtttgtcc gctcttctta ccacgccgat ttgcaggcga aagggatgga agttaagtaa
     2101 tcctgtaata attacttaca tctgtctgat aaaaaacccg ctttttgaag cgggtttttt
     2161 gtatcgaaca agatattgag ggagcgtcct gctcgccacg tcactcttta tgagagagct
     2221 tttcagcctg aagatcaacg tctgcgcctt ttttcgccgc agcatcgtca tcattcatcg
     2281 ccttcttgaa ccctttaatg gccgctccaa ggtctccgcc cagcgtacgt aacttcttag
     2341 tcccaaacag cagaacgacc agcgccgcaa ctactagcag tttggtaata ctaatctcac
     2401 ccatagatac cttcttgaca tataaccggc aacagaacgc gccttaaaaa ccagagaata
     2461 ttaacggtca ttcgacgcgc gcacacaata gcaatctgta acaaggtgaa tcaagcgccg
     2521 agtaaaaaaa catcataata attgcggcgg cgcaaagcga cggttgttta agacgggcag
     2581 ttgcgcgcgc acctgacgca cacgttcggg cgtcacttcc gccataatga gtgcaggcat
     2641 ttctgacgct gccgcaatgg tgacgccaaa gggatctata atccggcttt gaccgatatt
     2701 tttgttcccg cactcccccg ccgccaccat ataacaggtg gtatccagcg cacgagcggc
     2761 aagcaacgtt gaccaatgat gctctttgag cggcccgcga acccaggcgg caggaagtac
     2821 caggatttca gctccctgta atgcctgtgc cagcgccagc tctggaaagc gtaagtcata
     2881 acaggtcatc agaccgacct tcatcccctc cacctcgagt aacggagcga tttcattacc
     2941 agcatcaaca cggcgtgatt cctgaatggc aaatgcatca tagagatgca atttggcata
     3001 acgggcgacg atgtttcctg cctgtagcgc caccagcata ttccatgcgc gccccggcgt
     3061 tgaaggaaca tgaatcgtca gaattgtcgt catcatgtta cgtttacttt ctcgccgtaa
     3121 acgtccgagg aattcgcctt ccagcagctg tgctgattta accgatagat ctgcatcatg
     3181 atcatcgcgc gccagcaatg cttccggcag gacgaacagc gatacgtcgt tttccgccgc
     3241 ctgcgccatc aacgaggcac aaatctcagc gttcttttcc cacacagatg taacagcaaa
     3301 ctgtcctgcg gcaaccagca ttgccacctc ctgtcagagt cgtaaaattc gccaacgacg
     3361 attattcggc gttacactta tcactcatac aaatcaaata gcaggatttt gcagtgttac
     3421 aacttctttt agcagttttt attggcggtg gtacgggaag cgtggcgaga tggctgttaa
     3481 gtatgcgatt taacccgctg catcaggcga ttccgttggg gacgctggca gcaaacctga
     3541 ttggggcatt catcatagga atgggattcg cctggttcag caggatgacg aacattgatc
     3601 cagtgtggaa agtattaatc accaccggat tttgtggcgg tctaacaacc ttctcaacat
     3661 tttcggcaga agtggtgttt ttgttacaag agggccgctt tggctgggcg ttactgaacg
     3721 ttttcgtcaa ccttctgggg tcttttgcca tgacagcact ggctttctgg ctgttttcgg
     3781 cctcaaccgc acactaaaga ccaaaaaaaa acccgctgat taagcgggtt ttgaattctt
     3841 gctgacgtat cttacagagc gattacgttt gcagcagaag ggcctttggc accgttagtg
     3901 atttcgaact ctacgcgctg accttcagca agagttttaa aaccattagt ctggattgca
     3961 gagaagtgta cgaacacgtc tttgctgccg tcttccggag taatgaaacc gaatcctttg
     4021 gactcattaa accacttaac gttaccttta atcttagaca tcaaaattac ctttacatga
     4081 aaaatagaca caaatgctgt gtcggttacc agtacaccaa ttgtggtacg ctttgtccag
     4141 tgcaacttta ctaaaaagtg ataaaagtcg cgttattttt gatgttactt attttagcta
     4201 ttgattttaa agaagttact aaaacttcat ttgtctcaaa actgaaagcg catccaggca
     4261 aagtacacat tgccattgtt gtaggtaccc ggaatgtagg tcatctgaaa agtcactggg
     4321 ccataaccca cggaggccaa tggcagtaga accgggagag ggatgtaatt ccagttatcg
     4381 cgtgccgtta cgccagcggt gaatcccaga cctaaatgaa aattttcatc cgccagcggt
     4441 cgccaggtac tttcccatcc gtatccggca atcggttccc atttgttcca cgagtcctta
     4501 aatgccatgg catacaggcc atgccagttt cctttttcat cccaacgcga caggccaaaa
     4561 ccgccacccc acggtcgctc gttatagcga tcggtttttt ctttgtcgta agcgaaacgt
     4621 gcatgccagg tgatggcagg aatatataaa tcataatgtt caggctgttg ccaggtttgt
     4681 gcaatatttt ctctaaacgt tgtcatccac tcatctgcgt tagcaaaaac tttaccaacg
     4741 ctgattaact gaataaaaac aaaggaaaag atagcgacat atttactcac gttcatttgt
     4801 gaccataaaa catttat
//
LOCUS       DAZGJW010000249         4777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZGJW010000249 DAZGJW010000000
VERSION     DAZGJW010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.251"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..596)
                     /locus_tag="ACHKIK_004864"
     CDS             complement(<1..596)
                     /locus_tag="ACHKIK_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO9085867.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(711..786)
                     /locus_tag="ACHKIK_004865"
     tRNA            complement(711..786)
                     /locus_tag="ACHKIK_004865"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(751..753),aa:Thr,seq:ggt)
     gene            complement(793..867)
                     /locus_tag="ACHKIK_004866"
     tRNA            complement(793..867)
                     /locus_tag="ACHKIK_004866"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Gly,seq:tcc)
     gene            complement(984..1068)
                     /locus_tag="ACHKIK_004867"
     tRNA            complement(984..1068)
                     /locus_tag="ACHKIK_004867"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1032..1034),aa:Tyr,seq:gta)
     gene            complement(1077..1152)
                     /locus_tag="ACHKIK_004868"
     tRNA            complement(1077..1152)
                     /locus_tag="ACHKIK_004868"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1117..1119),aa:Thr,seq:tgt)
     gene            1514..2464
                     /gene="coaA"
                     /locus_tag="ACHKIK_004869"
     CDS             1514..2464
                     /gene="coaA"
                     /locus_tag="ACHKIK_004869"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO9085868.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2493..3458)
                     /gene="birA"
                     /locus_tag="ACHKIK_004870"
     CDS             complement(2493..3458)
                     /gene="birA"
                     /locus_tag="ACHKIK_004870"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO9085869.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMVEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3455..4483)
                     /gene="murB"
                     /locus_tag="ACHKIK_004871"
     CDS             complement(3455..4483)
                     /gene="murB"
                     /locus_tag="ACHKIK_004871"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO9085870.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1211 a         1218 c         1085 g         1263 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcatactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaagat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcgtat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cgcgagcagc accgccgtag
      481 gttttagcca gtacggtagt gattgcagcg gtcagcgttg ttttaccatg gtcaacgtgg
      541 ccgatagtac cgacgttaac gtgcggtttt gtacgttcaa acttttcttt agacatcgat
      601 tgtccctcta agacacggat aaatcggtga tatcaccaca tcaaccaggc aacatgcccg
      661 acttgttgaa tgcaataaac agaaggaaaa aacagggagg agaaaagaag tggtgctgat
      721 aggcagattc gaactgccga cctcaccctt accaagggtg cgctctacca actgagctat
      781 atcagcacat cttggagcgg gcagcgggaa tcgaacccgc atcatcagct tggaaggctg
      841 aggtaatagc cattatacga tgcccgcatc ctggaactcg gctacctgat tttcattctg
      901 cactgaatat cgagagaagc tctctttatt cgagccggta agcgaactta tcgtctcggg
      961 ctacgccatc gcgtggccga aattggtggt gggggaagga ttcgaacctt cgaagtctgt
     1021 gacggcagat ttacagtctg ctccctttgg ccgctcggga accccaccgg acttgatggt
     1081 gccgactacc ggaatcgaac tggtgaccta ctgattacaa gtcagttgct ctacctactg
     1141 agctaagtcg gcatcaagta gcgcgcattc tatggagaca tgcgagttca tgcaactaaa
     1201 aaattgcata atttgtttta ttggtcacat tttatgcgac acgatgaaga aacagccgaa
     1261 cggagggcag attagcacac tttttcaaca tcattgtgct caacaatgcg ctcctgctaa
     1321 accataattc tttttatcag atggaatatc tgtcacattg cttttcaacg atagcttcct
     1381 ggcagagatt ttttcttatt attcctcccc atctggtgtt accctcctgc ccattaaccc
     1441 attcaacaga actgtgacgc gccatggcaa atatcgcttt gccgatagag ctatgaccgc
     1501 cagaaacatg cttatgagta taaaagagca aacgttaatg acgccttacc tacagtttga
     1561 ccgcaaccag tgggcagctc tgcgtgattc cgtacctatg acgttatcgg aagatgagat
     1621 cgcccgtctc aaaggtatta atgaagatct ctcgttagaa gaagttgccg agatctattt
     1681 acctttgtca cgtttgctga acttctatat aagctcgaat ctgcgccgtc aggcagttct
     1741 ggaacagttt cttggtacta acgggcaacg cattccttac attatcagta ttgctggcag
     1801 tgtcgcggtg gggaaaagca caaccgcccg tgtattgcag gcgctattaa gccgttggcc
     1861 ggaacatcgt cgtgttgaac tgatcactac agatggcttc cttcacccta atcaggttct
     1921 gaaagaacgt ggtctgatga agaagaaagg cttcccggaa tcgtatgata tgcatcgcct
     1981 ggtgaagttt gtttccgatc tcaaatccgg cgtgccaaac gttacagccc ctgtttactc
     2041 gcatcttatt tatgatgtga tcccggatgg agataaaacg gttgttcagc ctgatatttt
     2101 aattcttgaa gggttaaatg tcttacagag cgggatggat tatccacacg atccacatca
     2161 tgtatttgtt tctgattttg tcgatttttc gatatatgtt gatgcaccgg aagacttact
     2221 tcagacgtgg tatatcaacc gttttctgaa attccgcgaa ggggctttta ccgacccgga
     2281 ttcctatttt cataactacg cgaaattaac taaagaagaa gcgattaaga ctgccatgac
     2341 attgtggaaa gagatcaact ggctgaactt aaagcaaaat attctaccta ctcgtgagcg
     2401 cgccagttta atcctgacga aaagtgctaa tcatgcggta gaagaggtca gactacgcaa
     2461 ataatttgca ggggagcgaa tactcccctt tcttattttt ctgcactacg cagggatatt
     2521 tcaccgccca tccagggttt tattattcca tcctgctcaa gtaataaagc cccctgttta
     2581 tctattccgc gtgaaatgcc aaatatttct ttatcaccaa tgataagttt cactgggcga
     2641 ttaataaaat tatccagctt ttcccagcgc gacagataag gtgccaatcc ttcttgttcg
     2701 aagagttcca acgcagcacg taattcacgt attagcatgg ccgccaacgt attacgatcg
     2761 agattgatcc ccgcttcctg cagcgtgatc cacccctgat taacgacact ctcttcaaca
     2821 cggcgcattg ccatgttgat cccggctcca atgactattt gcgccgcatc gccagttttg
     2881 ccagtcagct ccacaagaat gcctgccagc ttgcgatcct gcagatagag gtcattaggc
     2941 catttgacac gaactttatc tgctcccagc ttgcgtaata cttccaccat cacgataccg
     3001 ataaccagac ttaaaccaat cgccgccgcc gggccttgtt ccagacgcca gaacatcgac
     3061 aaatataagt ttgcgccaaa aggcgaaaac catttccgcc cccggcgacc acggccagcc
     3121 tgctggtatt ctgcaacaca ggcatcgccc gatttaagct ctccgatacg atcaagaagg
     3181 tactgattcg tggagtcaat aactggcagc acggctacac taccgccatc cagctgaccc
     3241 aatatctgtt cagcattaag taactggatg ggctcaggca ggctgtatcc tttacccgga
     3301 acggtaaaga catcaacgcc ccagtcacgc agtgtctgaa tgtgtttatt aatagccgcc
     3361 cggctcattc ccagcgtttc acccaactgc tcgccagagt gaaattcacc gttcgctaac
     3421 agggcaatca atttcagtgg cacggtgtta tccttcatga aattgtctcc actgcgctca
     3481 cttcacctga tgcaccaata aagcggactt caggctcaag ccagacatta aatttttcac
     3541 ccactttttg tcttacatgg tgtgccagtt gcaccacatc ttcgcttttt gcattgtctt
     3601 cattaatgag aactaacgcc tgttgacggt gcaccgcagc cccacccatt tgcatccctt
     3661 ttagctggca ctgatcaata agccaacctg ctgccagttt tactgaacca cccgcctggg
     3721 ggtaatttgg tgctgttgga aattgcgcca gtaatgcttt agccgtttcg gcagatacaa
     3781 cagggttttt gaagaaacta ccggcattgc cattcacttt tggatcaggg agtttggtgg
     3841 tgcgcatatg acacaccgca ttaaatactt gctgtggcgt tactgttgta ggatccagac
     3901 gagttaagtc accatacgtt agtacaggtt gccactcttt tggcagacgc agacctacgg
     3961 ctacaatggc gaagcgatcc tggtattcat gtttaaaaat actgtcgcga tagccaaaac
     4021 ggcactcttt ggcagttaag cgcacttgct tgcctgtcgc cagttcaaca caatcaacat
     4081 aagcgcaaac tcgctgtaat tctacgccat aagcaccaat attctggata ggtgatgagc
     4141 cgacacaacc aggaattaat gccagatttt ccagaccagg cataccttcc tgcaaagtgt
     4201 attttaccag gcgatgccag ttttctccgg ctcctacatg taaataccac gcatcaggtt
     4261 catcatgaat ttcgatacct ttgatccggt tgatgatcac cgtgccgcga tagtcttcca
     4321 gaaaaagtac attacttcct tcacccagaa taagaacggg ttgtccttct gcggttgcat
     4381 actgccaggc attgagtaat tgttgttcgt cttcggcaca tacaatgtgc tgagcattat
     4441 gatcaatgcc aaatgtgttc cagggtttta aggagtggtt catagctgct ttcctgatgc
     4501 aaaaacgagg ctagtttacc gtatctgtgg ggggatggct tgtagatatg acgacaggaa
     4561 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc
     4621 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc gcaacgttca
     4681 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4741 aacgaaaggc ccagtctttc gactgagcct ttcgttt
//
LOCUS       DAZGJW010000250         4763 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZGJW010000250 DAZGJW010000000
VERSION     DAZGJW010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.252"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            468..683
                     /locus_tag="ACHKIK_004872"
     CDS             468..683
                     /locus_tag="ACHKIK_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO9085871.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            683..1180
                     /gene="rrrD"
                     /locus_tag="ACHKIK_004873"
     CDS             683..1180
                     /gene="rrrD"
                     /locus_tag="ACHKIK_004873"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO9085872.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            1177..1614
                     /locus_tag="ACHKIK_004874"
     CDS             1177..1614
                     /locus_tag="ACHKIK_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO9085873.1"
                     /translation="MSRVTVIISALVICIIACQSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGLTSDAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
     gene            1764..2381
                     /locus_tag="ACHKIK_004875"
     CDS             1764..2381
                     /locus_tag="ACHKIK_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO9085874.1"
                     /translation="MLASLGGLVSCAAKVHFKEAGNAMNELIANHDFDFRQLVTAAEG
                     QPVTDTFQIARAFGKRHQHVIRAIKCLRCSEEFSTTHFWAVEKINDLGIFDKKQIYYR
                     MDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAVMLEYMKEK
                     DVASMSGRLLNRWGRTKKPQLLAKLERLERQGQFLLPGFDKGIQA"
     gene            complement(2569..2763)
                     /locus_tag="ACHKIK_004876"
     CDS             complement(2569..2763)
                     /locus_tag="ACHKIK_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO9085875.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
     gene            3159..3668
                     /locus_tag="ACHKIK_004877"
     CDS             3159..3668
                     /locus_tag="ACHKIK_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO9085876.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPVSNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEEDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            3640..>4763
                     /locus_tag="ACHKIK_004878"
     CDS             3640..>4763
                     /locus_tag="ACHKIK_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO9085877.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNCMGNDQIRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSARGDEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLD"
BASE COUNT         1248 a         1049 c         1249 g         1217 t
ORIGIN      
        1 tgctggagat aaatgcaccg gcagcataat aaaaaaagcc agcgactgac ctgaaaaaga
       61 agacgctggc taaaaggcct tatatgtttg tagagactta tttttcacag acagcaatga
      121 tgcctgtcaa tatattatca atatgcggat tgtttcagtt acagatgcct tattaaggaa
      181 aaaaacagcc agcactgact ttcggtggag aggtgctggc tcaaaaggat agatgtactt
      241 cacatgttgc ttctatatgg cagtacattt tctgacagac agtgacggat gttgtcaaga
      301 tattgtgtca tttataacct gaatcagggg aggccggaat gttatctggc atttttagca
      361 gagcctgaat gccataatca cggctcccgg cgttggccgt cagtgggtga cactggcggc
      421 ttttttgttt ttctttactt tcattttctg tcggcggtga cggagacata catcagatgg
      481 aaaaaatcac aacaggtgtg tcatacacca cgtcagcggt ggggacggga tactggttac
      541 tgcagctgct ggacaaagtc tctccgtccc agtgggtggc aataggtgtg ctgggaagtc
      601 tgctgtttgg cctgctgacg tatctgacaa atctttattt caagattaaa gaagataagc
      661 gtaaggctgc gagaggtgaa taatgtcgcc gtcattacgc aaggctgttg cagctgctat
      721 tggtggtggg gctgttgcca tagcgtctgt gctcatcact ggtccaggtg gtaacgatgg
      781 tctggaaggt gtcagctaca taccatacaa agatatcgtt ggcgtatgga ctgtatgtca
      841 cggacacacc ggaaaagaca tcatgcccgg taaaacgtat accgaagcag aatgcaaagc
      901 cctcctgaat aaagaccttg ccacggtcgc cagacaaatt aacccgtaca tcaacgtcga
      961 tataccggaa acaacgcgcg gcgctcttta ctcgttcgtt tacaacgtgg gcgctggcaa
     1021 tttcagaaca tcgacgcttc ttcgcaaaat aaaccagggc gatatcaaag gcgcatgtga
     1081 tcagctacgg cgctggacat acgctggcgg taagcaatgg aaagggctga tgactcgccg
     1141 cgagattgag cgtgaagtct gtttgtgggg gcaacaatga gcagggtaac cgttattatc
     1201 tccgctctgg ttatctgcat tatcgcctgc cagtcatggg ctgttaatca ttaccgtgat
     1261 aacgccatcg cctacaaaga gcagcgcgat aaagccacat ccatcatcgc tgatatgcag
     1321 aagcggcaac gtgatgtagc agaacttgac gccagataca caaaggagct tgctgatgct
     1381 aatgcgacta tcgaaagtct ccgtgctgat gtttctgctg ggcgtaagcg cctgcaagtc
     1441 tccgccacct gtgcaaagtc aacgaccgga gccagcagca tgggcgatgg agaaagccca
     1501 ggacttacat cagatgctga actcaattat taccgtctcc gaggtggaat cgacaagata
     1561 accgcgcagg ttaactacct gcaggaatac atcaggacgc agtgcttaaa ataattttaa
     1621 tttcactgaa atttaacaag tgactttcag gaaaatgcct cgcagatgcg gggcattttt
     1681 gtaccggtat ttcaccgcgc accgcagcgc acaataaaca ccgaacctga ccctttggaa
     1741 tgggcctttg aggataccag ttagtgctgg cgagcctcgg tgggctggtt tcctgtgcgg
     1801 caaaggttca tttcaaagaa gcaggcaacg ccatgaatga attaattgcg aatcatgact
     1861 tcgactttcg ccagttagtt accgcagcag aaggtcaacc ggtaactgac accttccaga
     1921 ttgccagggc atttggtaaa cgccatcagc atgtgattag ggctattaaa tgtttgagat
     1981 gttctgagga attctcgaca acccattttt gggccgtcga gaaaatcaat gacttaggta
     2041 tttttgacaa gaaacagatt tactaccgca tggactttag tggcttcgtt atgctggtta
     2101 tgggatttaa cggggcaaaa gccgatgctg ttaaagaagc ctatatcaat gcgtttaact
     2161 ggatgtcagc agaactccgt aagtacagcg aaagttatga agcagaacgt aacgccgtaa
     2221 tgctggagta catgaaagag aaggatgtcg ccagcatgtc aggccgtctg ctcaatcgct
     2281 gggggagaac gaaaaaacct caattgcttg caaagctgga acgtctggag agacagggac
     2341 agtttttatt accgggattc gataaaggta ttcaagcctg acacattatg cgctgtatcg
     2401 tcgccgtatt cccgcattaa ccatgaccgt agcccgacgg ggaattcctt ctgcgtgagt
     2461 gtgcgggaat aatcaaaaac gatgcacacc gggttttact gtgctgacag acgcagggtt
     2521 accctcatag tcgcttttcc ggtgcgatgg tggaagaaac cgggatgttc atccatcatc
     2581 actttggatt gatgtatatg ctctcttttc tgacgttagt ctccgacggc aggcttcaat
     2641 gacccaggct gagaaattcc cagacccttt ttgctcaaga gcgatgttaa tttgttcaat
     2701 catttggtta ggaaagcgga tgttgcgggt tgttgttctg cgggttctgt tcttcgttga
     2761 catgaggttg ccctgtattc agtgtcgctg atttgtattg tctgaagttg tttttacgtt
     2821 aagttgatgc agatcaatta atacgatacc tgcgtcataa ttgattattt gacgtggttt
     2881 gatggcgtag atgcacgttg tgacatgtag atgataatta ttatcatttt cgtgggtcct
     2941 ttccggcgat ccgacaggtt acggggcggc gacctcgcgg gttttcgcta tttatgaaaa
     3001 ttttccggga tccatgtccg gtttctcttc aagttaacta tatgaaaaat ataaaaacag
     3061 gtcttctgtg aaccggacat gaacaaaaaa cagacatgta aaccggacat gaccggtttt
     3121 gttgtgattg tgaggtgaga gtttttgcga ggtgaggagt ggctacgcag actgaagttg
     3181 ccaggcattt aagtctgacc gatcgccagc ttcgcagatt gcagaaatta ccgggtgccc
     3241 cggtctcgaa taagcgaggg caactggatc tggatgcctg gcgcgatttt tacatatcgt
     3301 atctgagaag aagtaaaaac gatgtgcctg atggcgatag cgaagaagac tatgaagaga
     3361 aattgcttat tgccagatgg gaactgacag cagaacaggc tgttacacag cagttaaaaa
     3421 atgaggtgtc aaaaggaaaa ctgattgaca ccgggttctg tatttttgcc ctcagtaagc
     3481 tggcaatggc gttatccagt acgcttgatt ccatcccttt atccatgcag cgacagtttc
     3541 ctgatttaac accgcgccat cttgaccatc tgaaaaccct tattgctaag ggggcaaatc
     3601 agtgtgcgcg ggcaggggat aaattaccgg atttacttga tgaatatatc agagcaacaa
     3661 ctgaataata tgatgagcgc tgtcacaact gcattacagc ccctgataag ggcattgccg
     3721 gtgacgccag ttgaatgggc tgatcaaaat tattatctgc ctaaagaatc ttcatatggt
     3781 gagggcgaat ggaaaacgct gccattccag atcgccatca tgaactgcat ggggaatgat
     3841 cagatccgca ctgttaatct gattaaatct gcccgtgttg gctatacaaa gatgttgctg
     3901 ggggtggtcg ggtattttat tgagcataaa tcccgaaaca gtctgctttt tcagcccacg
     3961 gattctgccg ctgaagattt tatgaagtct cacgtggagg cgacgattcg gaacgtgcca
     4021 tgcctgaaag acctttcccc atggctgggt cgtaaacatc gtgacaatac tctcacgctg
     4081 aaacgctttt catcgggcgt cggtttctgg tgcctgggcg gcgctgccgc caaaaactac
     4141 cgtgaaaaat ccgtggacgt ggtctgctat gacgaacttt cctcgttcga gccggatgtc
     4201 gaaaaagagg gctcgccaac cctgctgggg gataagcgta ttgaggggtc ggtgtggcca
     4261 aaatccattc gcggctcgac gcctaaaatc aaaggcacct gccagatcga aaaagccgct
     4321 aacgagtcgg cgcattttat gcgtttttat gtgccctgcc cgcactgtgg ggaggagcag
     4381 tatctgaaat ttggcgatga gtccacgcct tttgggctta aatgggagaa ggacagccct
     4441 gaaagtgttt tctacctctg tgaacatcat ggctgcgtga tccatcagtc tgaactggac
     4501 cagagcaacg ggcggtggat ctgtgaaaac acgggcatgt ggacccgtga cggtctgacg
     4561 tttttcagcg cccggggtga tgaaattccg ccgccgcgct ccatcacgtt ccatatctgg
     4621 acggcgtaca gtccgttcac cacctgggta cagattgtct atgactggct ggatgcactg
     4681 aaagatccca acggcctgaa aacctttgtg aacaccacgc tgggcgagac ctgggaagag
     4741 gccgtgggcg aaaaactcga tca
//
LOCUS       DAZGJW010000251         4746 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZGJW010000251 DAZGJW010000000
VERSION     DAZGJW010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.253"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..80
                     /locus_tag="ACHKIK_004879"
     CDS             <1..80
                     /locus_tag="ACHKIK_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085878.1"
                     /translation="GWFIGSIHDTEDGPVCVWLRNKAEA"
     gene            208..426
                     /locus_tag="ACHKIK_004880"
     CDS             208..426
                     /locus_tag="ACHKIK_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO9085879.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            428..985
                     /locus_tag="ACHKIK_004881"
     CDS             428..985
                     /locus_tag="ACHKIK_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO9085880.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            1276..1431
                     /locus_tag="ACHKIK_004882"
     CDS             1276..1431
                     /locus_tag="ACHKIK_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9085881.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            1551..1895
                     /locus_tag="ACHKIK_004883"
     CDS             1551..1895
                     /locus_tag="ACHKIK_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFO9085882.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            2017..2289
                     /locus_tag="ACHKIK_004884"
     CDS             2017..2289
                     /locus_tag="ACHKIK_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085883.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            2291..3340
                     /locus_tag="ACHKIK_004885"
     CDS             2291..3340
                     /locus_tag="ACHKIK_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9085884.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKIRSVYRESDIIPGEQTATSILKQR
                     TKNIALPPHTHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            3353..3727
                     /locus_tag="ACHKIK_004886"
     CDS             3353..3727
                     /locus_tag="ACHKIK_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9085885.1"
                     /translation="MRIEFVLLYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMLEGLVKK"
     gene            3724..4545
                     /locus_tag="ACHKIK_004887"
     CDS             3724..4545
                     /locus_tag="ACHKIK_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO9085886.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
BASE COUNT         1254 a         1051 c         1294 g         1147 t
ORIGIN      
        1 atggctggtt tattggttcc attcatgata cggaagatgg cccggtttgt gtctggttgc
       61 gaaataaggc tgaagcataa aggcgataaa ccaactaaca actaaatact gaagatttaa
      121 atcagaaacg atttttatta aatccttaac cggagggatt cctgcaccct cagaacatca
      181 ggaggccgcc cgaaagggcg gtaagaaatg aaacattatt tagaaaaaaa ttacccacga
      241 aagagcagaa caacagagtt tctgtttttc attctgttta tagtgttgat gataccgata
      301 tccccgctat tactggtctg gataattgga aggacatttg aaccagttat tgagctatat
      361 accgatgtga catgggaatc attcagcgca ctgcacaata aaattaatcc gtataaggaa
      421 aactgatatg agcactatta ccagagaacg cgcggagatt aaatcataca tcacaggctt
      481 cctgagcgac tcggcgcacg ataacaagtc ttcagacagc ctgctggcta atgtgtttcg
      541 tatcgcgctg gcatcactgg aagcagagcc gatagcaatg gtagtgcctg atgaaatgga
      601 tttgcttacc tgccatctcg acggtgtaac taaaacatat gctgatggct ggaacgcctg
      661 ccgcgtcgcc atgcttcagg ccggaaactt tcgggaaaat aagaattcgt caaccaacaa
      721 ttttcgggaa atctcggaaa cgtcaaccag atctccgata actctggatg gctggataag
      781 ctgtactgag cgaatgcctg aaaagagcca gaacgtgctt atttcgatga atatcgatag
      841 cgaggctggg ccattaatat attccgcacg ctatctcgga ggcacgttcc ggcgcggagg
      901 tatagcagtt agtccgggta atgatcttag gcaagcaacc cactggatgt cgctaccaga
      961 accgccgcag gaggtgaatc aatgacctgg cctgaagcat tcacaacggt aggaattgcg
     1021 atggcggtgg cgctggtggt gtattcgatt tgccgctggg gataaaaacg gtttgcggga
     1081 aaaggatagt taagtagaat tgcagcgggt gcttgaggct atctgcctcg ggcatgaaca
     1141 ccaacggcag atagagaaaa gccccagtta acattacgcg tcctgcaaga cgcttaacat
     1201 taatctgagg ctcaatccat gctgaacaca tgtaggttag cctcttacgt gccgaaaggc
     1261 aaggagaagc aggctatgaa gcagcaaaag gcgatgttaa tcgccctgat cgtcatctgt
     1321 ttaaccgtca tagtgacggc actggtaacg aggaaagacc tctgcgaggt gcgaatccga
     1381 accggtcaga cggaggtcgc tgtcttcgta gactacgaat ctgagaagta agagaccagg
     1441 cgggggagta atctcccgcc acctctgatg tgtcaggcat cctcaacgca cccgcgcttt
     1501 accatactga aaatgctgtt tgaatgttca tctctgaaag aggactatga atgaaaaagg
     1561 tattgattgc agcacttatt tccggtgtgt cttttggcgc ttttgcacag cagggtggtt
     1621 tccaggggcc agaagcagag cgttcaacag tagcgcaggc aaaagaactg aaggatgatg
     1681 catgggttat ccttgaaggg agcatcgtta aaaaagtggg tgatgaacgt tatgagtttc
     1741 gtgacaatag cgggacaatt gtcacggata ttgatgacag cgtatgggcc gggcagaatg
     1801 tttctccgaa agacaaagta agaattgagg gtgaaattga taaagacctg agcagtgttg
     1861 aagttgatgt aaaggcactg aaattattaa agtaaccgcc cctgcttgtt aagcccgtct
     1921 tactgacggg ttttctgttt gtacattccg gcgtattgcc ttacaattcg cgcagtcagc
     1981 ctgaacaact gacacctgct gtcaccggag aatccgatga cacaacacat aaaatcccac
     2041 aattctgaag ccgacccgga aattaagcag gggaggcgtt ttcgtgcgcc tcagtatggc
     2101 tggtttcact atctgttctg tacgatcgat gaggcagata tgcttcaaga ggcgtatctg
     2161 cgtcgcggtg tccgtgtgga gcggagtctg aacgctgatc gtctgacctg gaccgtttct
     2221 gtatatcttc ctgttcgtgc acatctgcca cggacacatg cctgctaccg tcagcgcgtc
     2281 tggaggtaat gtgcgggtat tacttcgacc tgttctggtt ccggaactcg ggctggtggt
     2341 ccttaagccc ggtcgtgaat cattgccagt ttttcatcgc ggcagggtgc tggtggagcc
     2401 ggaaccgaaa aacatgcggg cgctgccatc tggagcggtt cctgctgttc gccagccgct
     2461 ggcggaagat aaatcactgc tgccattttt cagcgatgag cgggtgattc gtgcagctgg
     2521 cggcgctggt gcactgtctg actggttatt acgtcacgtg aaatcctgcc agtggccaca
     2581 cggcgattat catcacagcg aaaccgttat tcacagttac ggtgctggcg caatggtgtt
     2641 gtgctggcac tgcgacaacc agctgcgcga ccagacctcc gaatcacttg agcaacttac
     2701 tcaacaaaat ctgacagcct ggatgattga cgtcatacgc catgtaatga atggcacgca
     2761 ggagcgggaa ttatcgctgg ctgaattatc ctggtgggca gtctgcaatc aggtggtgga
     2821 cgcattacct gaggcagtat cgcgtcgctc tctgggatta ccggcggaaa aaatccgctc
     2881 cgtataccgt gaaagcgaca tcataccggg agaacagacc gccaccagca tactgaagca
     2941 gcgcacaaaa aatattgcgc taccgcctca cacccaccag caacagaacc caccacagga
     3001 aaagacggtg gtcagcattg ccgttgatcc ggagtctccg gaatccttca tgaaacgacc
     3061 taaacgtcgc cgctgggtaa atgagaaata cacacgctgg gtaaagacac agccgtgtgc
     3121 gtgttgtggt aagccagcgg acgatcctca tcatctgatt ggtcatggtc agggtggaat
     3181 gggaacaaaa tcccacgata ttttcacgct accgctgtgt cgggagcatc acaacgagct
     3241 tcatgcggat ccgctggcgt tcgaagaaaa gcatggttcc caggttgatt taatttttcg
     3301 ttttcttgat cacgcctttg caaccggcgt gctcgggtaa aagaggttac tgatgcgtat
     3361 agagtttgtt ttgctttacc cgccgacggt gaacacctac tggcgacgtc gtggcagcac
     3421 atattttgta tcaaaagccg gtgagcgtta tcgccgggct gtggcgctta ttgttcgcca
     3481 gcagcggctg aaattaagcc tgtccggaag gctggcgata aagattattg ccgagccacc
     3541 ggataagcgc cgccgtgacc tggacaatat tctgaaagcg ccgctggatg cgctgacgca
     3601 tgcgggagtg ttaatggacg atgagcagtt tgatgaaatc aatatcgttc gtggtcagcc
     3661 agtatctggt ggacgtctgg gggtgaagat ttaccccata atgcttgaag ggctggtcaa
     3721 aaaatgaaac tggaagattt accgaaatac tactccccaa aatcccccgg cctgactgat
     3781 gcatcggcct caacgtcgaa agatacgctg agtatcactg atgtgatggc cgcgcagggc
     3841 atgacacaga attgggctga gatggggttt tctgcgttcc ttgggaaaat gggcattagt
     3901 atgaatgaca gagagcgggc aacagaattg ctgacagaat atgcactcag tcggtgtgat
     3961 cgcgtggcgg cgttaagaaa actcccggca gaaataaaac cggcagtgat gcgtattatg
     4021 gcttcgtatg cgtttgaaga ttatgcccgt agcgcggcga gcaaaaaaca gtgcccctgt
     4081 tgtcacggaa aaaaatttat tgaaagcgag gtttttacaa acaagatcca gtatccggat
     4141 ggtaagccgc cagtgtgggc aaagtgcaca aaaggcgtgt atccgtctta ctgggaggaa
     4201 tggaaaaaag tcagggaggt ggtaaaagtt gcctgtccgg agtgtggagg gaagggggag
     4261 gtttccaccg cctgtaaaga ttgtcgtggg cgcggtgttg ccattcatcg tgaagagtcg
     4321 gtaaaacgtg gtatgcctgt tatcagagac tgccagcgtt gtggtggtcg tggctatgaa
     4381 agattacctt caacggaggc atttaatgcc atatgtaatg taaccgatgc catatctctt
     4441 gatacatgga aaaaaacagt taaacgtttt tacgatacgc tggtggtgca gtttgatatt
     4501 gaagaagcat gggcagaaca acaactgaaa aaggtgacca gatagctttg ttgatttttc
     4561 ccgaatctgt ggtaaatttg ccctaacgat gggcgtttta tgcctgacgt tagaagattt
     4621 tttacacccg tcgccaggcg ggttttttta tgactgaaat cacgccagta cagtaaacgc
     4681 gctggtggtt gtgaataccg gtctttcagc ttgctggctt tttcgacaag agttattggt
     4741 gtgtca
//
LOCUS       DAZGJW010000252         4676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZGJW010000252 DAZGJW010000000
VERSION     DAZGJW010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.254"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            31..1089
                     /gene="tsuA"
                     /locus_tag="ACHKIK_004888"
     CDS             31..1089
                     /gene="tsuA"
                     /locus_tag="ACHKIK_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO9085887.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            1103..1330
                     /gene="tsuB"
                     /locus_tag="ACHKIK_004889"
     CDS             1103..1330
                     /gene="tsuB"
                     /locus_tag="ACHKIK_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO9085888.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1373..2800)
                     /gene="sbcB"
                     /locus_tag="ACHKIK_004890"
     CDS             complement(1373..2800)
                     /gene="sbcB"
                     /locus_tag="ACHKIK_004890"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO9085889.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQETRAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFVEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            3009..4175
                     /gene="dacD"
                     /locus_tag="ACHKIK_004891"
     CDS             3009..4175
                     /gene="dacD"
                     /locus_tag="ACHKIK_004891"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO9085890.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRTIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHDEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            4293..>4676
                     /gene="sbmC"
                     /locus_tag="ACHKIK_004892"
     CDS             4293..>4676
                     /gene="sbmC"
                     /locus_tag="ACHKIK_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO9085891.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVFYDNPDETPAEKLRCDIVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPK"
BASE COUNT         1134 a         1056 c         1228 g         1258 t
ORIGIN      
        1 cccattaaca ttcatctggt taattaattt atgttttcaa tgatattaag cgggctaatt
       61 tgtggtgctc tgctgggatt tgtcatgcag cgtgggcgtt tttgcctgac aggtggtttt
      121 cgcgatatgt atatcgcgaa aaataatcgc atgttttacg ccttgctgat tgcaatttcg
      181 gtacaaagcg tgggggtttt tgcgttaatt caggcgggcc tactgactta cgaagccggg
      241 gcgttcccgt ggcttggtac tgttataggt gggtatcttt tcgggctggg aattgttctg
      301 gcgggtggat gtgccaccgg gacctggtat cgcgcgggtg agggattgat cggcagttgg
      361 atcgcgcttt tcacttatat ggtgatgagt gcggtgatgc gttctccaca cgccagtggt
      421 ttaaatcaaa ccttgcagca ctacactact gaacataact ctattgctga tacttttaat
      481 ttgtctgtgt ggccgttggt tgccgttttg ctggtgataa cgctctgggt ggtgatgaaa
      541 gagttgaaga agccaaaact caaagtcgcg accttaccgc cgcgccgaac cgggatcgct
      601 catattctgt ttgaaaaacg ctggcatccc tttgtcactg ctgtactcat cggtttgatt
      661 gcgcttttag cttggcccct gagtgaagca accggacgca tgtttgggtt aggaatcact
      721 tccccaacgg ccaatattct gcaatttctg gtcgcgggtg acgtgaaata cattaactgg
      781 ggcgttttcc tggtgttagg gatcttcgtg gggtcattta ttgcagccaa agcgagccgt
      841 gagttccgcg ttcgcgctgc tgatgcacaa acaacattac gtagcgggtt aggtggtgta
      901 ctgatgggct tcggtgccag cattgcaggt ggttgctcta tcggtaatgg actggtcatg
      961 actgcaatga tgacctggca gggctggatt ggccttgtat ttatgattct cggagtctgg
     1021 actgcgtcct ggcttgtgta tgttcgaccg cagcgtaagg cacgactggc aactgctgcg
     1081 gcaaattaag taaggtgtaa agatggcgat taaaaagctg gatgtggtga cgcaggtctg
     1141 tccgtttcca ctcatagaag caaaagcagc actggcagaa atggccagcg gcgatgagtt
     1201 ggtaattgag tttgattgca cccaggcaac tgaagcaatc ccacagtggg cggcagaaga
     1261 aggacatgcc atcaccgatt atcagcagat tggcgatgca gcgtggagca tcaccgttca
     1321 aaaagcctga ctcaaaattg tcctccgccg taccagcggc ggaggcttca cattagacaa
     1381 tctcttccgc gtactgccaa agcgctttta acagcgccac tttttcttta tcgtcggcat
     1441 actgctgcgc cagcatctga atctcttctg catagccttg taaaaactct ggcgtgaaga
     1501 cctggcggcg gtgctccagc cagcgttgct gctcggcata atccagcgtc cccgggaagt
     1561 tgcgcgcccg ataattgaac aacagctttt caattcgttt atcaacaaaa gtgatatcca
     1621 gtgccggtaa attacgcggc tcggtttcca gcacaatttt cattgctgca cgatcggcgt
     1681 cactgaaaaa gccgttataa agctgtgcat ccacattatc tgaaggtgta aacggttcgg
     1741 cttccacgaa tatcgctacc actttttcac gcacttgcgg attttcacgc agaattttta
     1801 ggttatcgag gcaatgctga cgattaattc ccagtcggtc ggcatcttcc gggcgtagcg
     1861 tattcgcctg ggccagtacc ggacatttat tgatatgtac cagcttaacc ggaacggcgg
     1921 cgttatcgcc aagatcggct tttgcggtat ataaacgctc gcgcaatgtg tcgctatcca
     1981 gctccagtaa tggcgaaatg tctcctgcca aatccaccat aattacggca ttgcgatttt
     2041 caggatgcca cgccagcggt gccacccagc ttgtattgcc gcgccatgca ccaaacattc
     2101 cggaaacgtg aaccaggggt ttcatctgag gaacatcaat caacgccatt agtttgtgtt
     2161 tattacgatg ggtaaagaga taatcaaaca ggcgtggctg acgcgttttt accagttttg
     2221 ccatcgcaat agtggcgtac acatcagcca tcgcatcgtg ggcgttgcta tgttcaatac
     2281 cattcgcttt ggttaaatgc tcaaggcgaa agctcggtag accgtcatca ttttcaggcc
     2341 aatttattcc ttccgggcgc agggcataac aggcacgcat aacatccagt aaatcccagc
     2401 gcgagttatc atgctgccag ctccaggcgt aaggatcgta gaaattacga taaaaaacgt
     2461 tgcgtgtgac ttcgtcgtcg aaacgcacat tgttgtagcc cagaatacag gtcttcggta
     2521 cggtaaaaag cgagtgaata cgggcggcaa acgcggcttc gttttctcct ttcgctcgtg
     2581 tctcctgcgg ggtaataccg gtaattaata ctgctccagg ctggggtaaa tagtcatccg
     2641 cgggcttgca gtaaaagact tcgggttcgc cgatgacatt gaattcgtca tcggtgcgaa
     2701 tggctgcgaa ctgtgcaggg cgatctaacg cggggtgcgt gccaaaggtt tcgtaatcgt
     2761 gaaacaaaaa ggtagattgt tgcttaccgt cattcatcat taggttaaat ccgttatttc
     2821 tgctgtctgc cagagtatca aatatcaccg tgctaatcag ctttagcgcg acaatttgac
     2881 agcgagtgac aacagatcat gtcagataaa aatgagaggg tagtcacatt ttcttgcact
     2941 ttattccagc cagttcataa gtatttccgt aaaaagaaca gctatttgaa actcctgagg
     3001 gtttgctgtt gaaacgccgt cttattattg ctgcttcttt gttcattttt aacttatcgt
     3061 ctggttttgc ggcggaaaac attccttttt cacctcagcc tccagagatt catgccggct
     3121 cctgggtact gatggattac accaccggtc agatcctcac cgcgggtaat gagcatcaac
     3181 agcgcaatcc cgccagcctg acaaagctga tgacgggcta tgtcgtggat cgcactatcg
     3241 atagtcatcg cattacgcca gacgatattg tcaccgtggg gcgcgatgcg tgggcgaaag
     3301 ataatccagt gtttgtcggt tcttcactga tgtttttgaa agagggcgat cgcgtatcgg
     3361 tacgtgattt aagccgtggt ttaattgtgg attccggaaa tgacgcttgt gttgcactgg
     3421 ctgactatat tgccggtggg caacggcagt ttgttgaaat gatgaacaac tatgccgaga
     3481 agctgcatct caaggatacg cattttgaaa cagtgcatgg tctggatgca ccaggtcagc
     3541 atagctcggc ttatgattta gctgtgcttt ctcgcgctat catccacgac gagcccgagt
     3601 tttatcatat gtacagtgag aaaagtctca cctggaacgg tatcacccag caaaaccgta
     3661 acgggttatt gtgggataaa accatgaatg ttgacggcct gaaaacgggt catacttctg
     3721 gtgccgggtt taatctcatt gcttcggctg tcgatgggca gcgtcgtctc attgcagtgg
     3781 taatgggggc tgacagtgca aaaggtcgtg aggaagaggc aagaaaatta ctgcgttggg
     3841 ggcaacaaaa ctttactacg gtgcaaattt tgcaccgtgg gaaaaaggtc ggtacggaac
     3901 gcatctggta tggcgataaa gaaaatatcg acctgggaac ggaacaagag ttctggatgg
     3961 tgctaccgaa agccgaaatt ccacatatca aagccaaata tacccttgat ggtaaagagc
     4021 tcaccgcgcc aattagcgcc catcagcggg taggggaaat tgaactttac gaccgtgata
     4081 aacaggtggc gcactggccg ctggttaccc tggaatctgt cggggaaggc agcatgtttt
     4141 ctcgcctgag tgattatttc caccataagg cctgaccttt cttttgcagc agactggcag
     4201 gagtgcgagt ctgctcgcat aatcaacact cattccttgt ggttttaata tcgcaattat
     4261 actgtatata aaaacagtgt taatgggacg tcatgaacta cgagattaag caggaagata
     4321 aacgtaccgt tgcaggtttc catctcgttg gcccgtggga acagacggta aagaaaggct
     4381 ttgagcagtt gatgatgtgg gtagatagca aaaatattgt gccgaaggag tgggttgctg
     4441 tcttttacga caatccagat gaaacacccg ccgaaaaatt acgctgcgac atcgtcgtga
     4501 cggtgccgaa taactttacg ctccccgaaa acagtgaggg cgtcattctg acagaaattt
     4561 caggtggtca gtatgcggtt gcggtggctc gtgtagtcgg tgatgatttt gctaaaccct
     4621 ggtatcagtt ctttaatagc ctcttgcagg acagtgctta tgaaatgtta ccaaag
//
LOCUS       DAZGJW010000253         4562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZGJW010000253 DAZGJW010000000
VERSION     DAZGJW010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.255"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..658
                     /gene="paa"
                     /locus_tag="ACHKIK_004893"
     CDS             <1..658
                     /gene="paa"
                     /locus_tag="ACHKIK_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFO9085892.1"
                     /translation="AAKLYAEKTGIIVNVHYGPQNKWNEDAKKNADILFGASEQSALA
                     IIRDHKDSFSEKDIQPLYLRKSILLVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNT
                     SGTGVWEDIAGRKGNIETVAAIRKNIILYAPNSGTARKALENQPGADVWITWADWAAS
                     NPEIGDVVEIAPDYVIWRDMNITVRQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT
                     "
     gene            994..1149
                     /gene="hokD"
                     /locus_tag="ACHKIK_004894"
     CDS             994..1149
                     /gene="hokD"
                     /locus_tag="ACHKIK_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO9085893.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1370..1627
                     /locus_tag="ACHKIK_004895"
     CDS             1370..1627
                     /locus_tag="ACHKIK_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085894.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            1697..1975
                     /locus_tag="ACHKIK_004896"
     CDS             1697..1975
                     /locus_tag="ACHKIK_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085895.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            1977..3032
                     /locus_tag="ACHKIK_004897"
     CDS             1977..3032
                     /locus_tag="ACHKIK_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9085896.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            3033..3398
                     /locus_tag="ACHKIK_004898"
     CDS             3033..3398
                     /locus_tag="ACHKIK_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9085897.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRHGNTYFISEAGKRYRRDVALIVR
                     QQRLKLNLSGRLVIKIIAEPPDKRRRDLDNILKAPLDVLTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELECA"
     gene            3395..4084
                     /locus_tag="ACHKIK_004899"
     CDS             3395..4084
                     /locus_tag="ACHKIK_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO9085898.1"
                     /translation="MNNHYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKA
                     AFSKQSVAKVD"
     gene            4280..4355
                     /locus_tag="ACHKIK_004900"
     tRNA            4280..4355
                     /locus_tag="ACHKIK_004900"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4313..4315,aa:Ile,seq:cat)
     gene            4446..4519
                     /locus_tag="ACHKIK_004901"
     tRNA            4446..4519
                     /locus_tag="ACHKIK_004901"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4480..4482,aa:Arg,seq:tct)
BASE COUNT         1217 a         1037 c         1222 g         1086 t
ORIGIN      
        1 tgcagccaaa ctttatgccg aaaaaacagg tattatagtg aacgttcatt acggcccaca
       61 gaacaaatgg aatgaagatg ccaaaaaaaa tgcagatatc ttgtttggcg catcagaaca
      121 atctgctctg gctatcattc gggaccataa agacagcttc agtgaaaaag atattcagcc
      181 tctttatctg cgaaaaagta ttttactggt aaagaaaggt aatcctaaaa atatccggag
      241 tattgacgac ctgaccagac ctgggattgg cgtaattgtt aatgatggtg gtggtaccag
      301 taatacatca ggcactggcg tctgggaaga tattgccgga cgtaaaggga atatagaaac
      361 tgtcgccgca atccgaaaaa atattatttt atatgcgccc aatagcggaa ctgcacgtaa
      421 ggctcttgag aatcagcctg gagcagatgt ctggataacc tgggctgact gggcagccag
      481 taatccagaa attggtgatg tcgtggaaat agcgccagac tacgtgatat ggcgtgatat
      541 gaacattaca gtacgtcagg atgcaaatga tgaaacccgt cgatttgcag aatggctaca
      601 aaccgatgaa gcggcgcctg cattcaaaaa atatggctgg accaggaaag gcacttgaca
      661 tcctcgtcct tcaggacgtg gattcttttt ccggatgccg cgccagcggc atgtaggggc
      721 agctcacaaa acggaaaaaa ttgtacgcta agcctcgcca ggtgaactga attcattccg
      781 atatgggaat tcccatatcg ggcgaaaacg gtttgctgta acggcagagt taagtaggat
      841 tgctgcgggt gcttgaggct atctgcctcg ggcatgaaca ccaacggcag atagataaaa
      901 gccccacccg actataaatc gaagtgaggc ccctatatgc tcgtcacata tagattgcct
      961 cttacggacc gaaaggtcaa ggagaagcag gctatgaagc agcaaaaggc gatgttaatc
     1021 gccctgatcg tcatctgttt aaccgtcata gtgacggcac tggtaacgag gaaagacctc
     1081 tgcgaggtac gaatccgaac cggccagacg gaggtcgctg tcttcacagc ttacgaacct
     1141 gaggagtaag agaccaggcg ggggagaaat ccctcgccac ctctgatgtg tcaggcatcc
     1201 tcaacgcacc cgcacttaac ccgcttcggc gggtttttgt ttttattttc aacgcgtttg
     1261 aagttccgga cggcgccgga atagaatcaa aaatacttaa gtagcgcgca gggagaagag
     1321 ggatggaccc cgaacagggg agtgctattt atctggaagg attctgttga tgagaatcga
     1381 agaattacgt gaaattttta gtgaagatgg cctctatact gtgcgcgttg agaagggcgc
     1441 tattgtcagc cactgccgta ttaaatgttt acagtctcaa caaaggaaga gtggagctgc
     1501 gttaattcat tttgtggatg ggcttgtgac ggatggtttt attttgcgtg caaatgaatt
     1561 tgtcacatcg ttgccgtctc tgaaagaagc tgggattaag gctggttttt ctgcttttga
     1621 agattagtga attcatctac aattcagcgc agggctgaac ccctgttgag taacactgtg
     1681 ccaccggaga aagccgatgg cgcaaaattc cagactacac aattctgata attcagccgt
     1741 ctttgccagc aggcacgggc ggcgttctca tgcattcaaa tctgactggt tccggcacgc
     1801 cccatgcact gaagaacagg ccgaatggct gattcagaac taccgcagac gtgggtatga
     1861 gtttaggaaa gccctcagcc tcgattatcg tcactggata atctacgtca ggctgccgta
     1921 ctccgagcgc ccaccgcgtc cgtcccgcac attccagcaa cgcatctgga ggtaacgtgc
     1981 gggtattact tcgacctgtt ccggtaccgg aacttgggtt ggtggtcctt aaaccaggcc
     2041 gtgaatccat gcaggtattc cataaccctc gagtgcttgt ggagccggaa ccgaaaagca
     2101 tgcgcggtct gccgtccggc atcgtccctg ccgttcgcca gccgctggcg gaggataaat
     2161 cattactgcc gtttttcagc aatgagcgtg tgattcgtgc tgttggtggc gctggtgcac
     2221 tgtctgactg gctgttgcgt catgtcaaat cctgccagtg gcctcatggt gactatcatc
     2281 acagtgaaac cgttattcac cgttatggta ccggcgcaat ggtgttgtgc tggcactgcg
     2341 acaaccagct gcgtgaccag acatccgaat cactcgagca acttgctcat caaaacctgt
     2401 cagcatggat gattgacgtc atcggtcacg caataagcgg tacgcaggag cgtgaattat
     2461 ctctggctga attatcctgg tgggcggtcc gcaatcaggt ggcggacgcg ctaccggaag
     2521 cggtattacg tcgttcgctg gggttgcgtg cggaaaaaat ccgctcaatg taccgtgaaa
     2581 gcgacatcgt accgggagag cagaccgcca ccatcatact gaagcagcgc acaaaaaatc
     2641 ttgcgccgct gcctcacgcc caccagcaaa acccgccaca ggaaaagacg gtggtcagca
     2701 ttgccgttga tccggagtca ccggctcagt atctccagcg ccagaaacca caacgggaag
     2761 agatgcctgt atacacgcgc tgggtaaaaa cgcagaaatg catgacgtgc ggtaatcagg
     2821 cagatgatcc gcatcacatc attggtcatg gactgggagg gatgggaaca aaggctgatg
     2881 atttgtttgt tattccgctg tgccgtaaat gccatagcga actacacgcc ggggtaaaag
     2941 attttgaaga aaaacacggc agccagctgt tgttgctgat tcgtttttta atgcacgcga
     3001 gaaattcggg tgtcctgaag tggaaagcat gaatgactga acggatagaa tttgttttgc
     3061 cttacccgcc gacggtgaat acctactggc gacgtcatgg caatacgtat ttcatctcgg
     3121 aagccggaaa gcgttatcgc cgtgatgtgg cgctaattgt tcgccagcag cggctgaaat
     3181 taaacctgtc cggaaggctg gtgataaaga ttattgcaga gccaccggat aagcgccgtc
     3241 gtgacctgga caatatcctg aaagcaccac tggatgtgct gacgcatgcc ggacttctca
     3301 tagacgacga gcagtttgat gaaatcaata ttgtgcgcgg tcagctcgtt cctggtgggc
     3361 ggctggggat aaaaatcaca gaactggagt gcgcatgaat aaccactatt tacagtttgt
     3421 gcgtgagcag ctcattatcg ccaccgctga tttgagtggg gcaacaaaag gtcagcttga
     3481 agcctggcag gagaatgcca tgttcgatac agggcgttac aggcgaaaaa aaatccggta
     3541 ccgcgatgaa gtgactggaa aaatgataac gcgggataat ccaccaatcc cgggaaagca
     3601 atcgctggcg aaggtgacgt caattcctct ggtcagtccg gttgagtttt cgacatcatc
     3661 gtggcggcgg gctgttctgt ctcttgaaga acatcataaa gcctggttgt tgtggtgtta
     3721 cagcgggagt atttgttggg aatatcagat cgcgataaca cagtgggcgt ggaatgaatt
     3781 taatactcaa tccggtacca gaaaaattgc tgggaaaacg caggaacgcc tgaaaaaatt
     3841 aatctggctg gcggcgcagg cagtaaaagc agaacttttt ggtggggaag gttatgaata
     3901 tcaggagctg gcattactgg cgggagtgac aactaaaaac tggtccaaaa catttactcg
     3961 tcactgggtt gcaatgaaac acatttttca ccgactggat agtgaggctt tattatttgt
     4021 aatgagaaca cgttcaaaac aaaaggcggc attttcaaag caaagtgttg caaaagtaga
     4081 ttgaaaggca tatatttcat gcaaatctga tattttgccg attttgtacg tgatggcaaa
     4141 agcaaacaaa acccgcccac aagcgggttt tgttgtgcca cttatctcgg atagacatgg
     4201 tgaatgcgct ggtggaggag ataagggtga tttttgtatg cttgcaacat tgatttcgta
     4261 acgttattat cctgcgcccg gccctttagc tcagtggtga gagcgagcga ctcataatcg
     4321 ccaggtcgct ggttcaaatc cagcaagggc caccaaccgc cactagccat caggaaagag
     4381 cgtcaaccct ttaagttgag tgtgcgaggt tcgagtcccc ggtggtggtc cattatcggt
     4441 attctgcgtt gttagctcag caggacagag caattgcctt ctaagcaatc ggtcactggt
     4501 tcgactccag tacaacgcgc tacacttatt ttcccggctc gcttttgcgg gccttttttg
     4561 ta
//
LOCUS       DAZGJW010000254         4561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZGJW010000254 DAZGJW010000000
VERSION     DAZGJW010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.256"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            274..4527
                     /gene="fdeC"
                     /locus_tag="ACHKIK_004902"
     CDS             274..4527
                     /gene="fdeC"
                     /locus_tag="ACHKIK_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO9085899.1"
                     /translation="MSHYKTGHKQPRFRYSVLTRCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWHDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     AFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAVGSTASGW
                     TDNGDGTWTAQISLGTTAGELEVMPKLNGQDAAANAAKVTVVADALSSGQSTVSVAAD
                     RVKAGESTTVTLIAKDAHGNAISGLSLSASLTGAASEGATVSGWTEKGDGSYVATLTT
                     GGKTGELLVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTKLTFTV
                     KDAYGNLVTGLKPDAPQFSGAASTGTERPSTGDWTETSNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVDNQLANGQSTNQVTLTVVDTYGNPL
                     QGQNVTLTLPKGVTSKTGNTVTTDAAGKADIELMSTVAGEHSITASVNNAQKTVTVKF
                     NADASTGQANLQVDTAAQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
BASE COUNT         1209 a         1069 c         1271 g         1012 t
ORIGIN      
        1 ccgcagagta agaaatataa cttaggtatc tatttaatga cttgcacaaa aagctaaatt
       61 ttcccccata aataaaaata taatcccgcg cccaaccacc tgatgagtgg ctacaggcac
      121 tggatatatt aggtggcggt gcactttctt acataaaggt atttcctttt ctgcggaaaa
      181 ggaaatcggg aaatccccgg tttttctgac aagcagacgc cattatttgt gtctgcctat
      241 gttcgttaat tcgttcatca ggaaattatc tcaatgtcac attataaaac aggtcataaa
      301 caaccacgat ttcgttattc agttctgacc cgctgcgtgg cgtgggcaaa tatctctgtt
      361 caggttcttt ttccactcgc tgtcaccttt accccagtaa tggcggcacg tgcgcagcat
      421 gcggttcagc cacggttgag catgggaaat actacggtaa ctgctgataa taacgtggag
      481 aaaaatgtcg cgtcgtttgc cgcaaatgcc gggacatttt taagcagtca gccagatagc
      541 gatgcgacac gtaactttat taccggaatg gccacagcta aagctaacca ggaaatacag
      601 gagtggctcg ggaaatatgg tactgcgcgc gtcaaactga atgtcgataa agatttctcg
      661 ctgaaggatt cttcgctgga aatgctttat ccgatttatg atacgccaac aaatatgttg
      721 ttcactcagg gagcaataca tcgtaccgac gatcgtactc agtcaaatat tggttttggc
      781 tggcgtcatt tttcaggaaa tgactggatg gcgggggtga acacctttat cgaccatgat
      841 ttatcccgta gtcatacccg cattggtgtt ggtgcggaat actggcacga ttatctgaaa
      901 ctgagcgcca atggttatat ccgggcttct ggctggaaaa aatcgccgga tgttgaggat
      961 tatcaggaac gcccggcgaa tggctgggat attcgtgctg agggctattt acccgcctgg
     1021 ccgcagcttg gcgcaagcct gatgtatgaa cagtattatg gcgatgaagt cgggctgttt
     1081 ggtaaagata agcgccagaa agacccgcat gctatttctg ccgaggtgac ctatacgcca
     1141 gtgcctcttc tgacactgag cgccgggcat aagcagggca agagtggtga gaatgacact
     1201 cgctttggcc tggaagttaa ttatcggatt ggcgaacctc tggcgaaaca actcgataca
     1261 gacagcattc gcgagcgtcg ggtactggca ggcagccgct atgacctggt tgagcgtaat
     1321 aacaacatcg ttcttgagta ccgcaaatct gaagtgatcc gtattgctct gcctgaacgt
     1381 attgaaggta agggtggtca gacactttcc ctggggcttg tggtcagcaa agcaactcac
     1441 ggactgaaaa atgtgcagtg ggaagcgccg tcattactgg ctgagggtgg caaaattacc
     1501 ggtcagggta gtcagtggca agtaacgctc ccggcttatc gtccaggcaa agacaattat
     1561 tatgcgattt ctgcggttgc ctacgataac aaaggcaatg cctcaaaacg cgtgcagaca
     1621 gaggtggtca ttaccggagc aggtatgagc gccgatcgca cggcgttaac gcttgacggt
     1681 cagagccgta ttcaaatgct tgctaacggt aatgagcaaa gaccgctggt gctgtctctg
     1741 cgcgacgccg aggggcagcc agtcacgggc atgaaagatc agatcaagac tgaactagcc
     1801 ttcaaaccgg ctggaaatat tgtgactcgt tccctaaagg ccactaaatc acaggcaaag
     1861 ccaacactgg gtgagttcac cgaaactgaa gcaggggtgt atcagtctgt ctttactacc
     1921 ggaacgcagt caggtgaggc aacgattact gttagcgttg atggcatgag caaaaccgtc
     1981 actgcagaac tgcgggccac gatgatggat gtggcaaact ccaccctgag cgctaacgag
     2041 ccgtcaggtg atgtggttgc tgatggtcag caagcctata cgttgacgtt gactgcggtg
     2101 gactccgagg gtaatccggt gacgggagaa gccagccgct tgcgatttgt tccgcaagac
     2161 actaatggtg taaccgttgg tgccatttcg gaaataaaac caggcgttta cagcgccacg
     2221 gtttcttcaa cccgtgccgg aaacgttgtt gtgcgtgctt tcagcgagca gtatcagctg
     2281 ggcacattac aacaaacgct gaagtttgtt gccgggccgc ttgatgcagc acattcgtcc
     2341 atcacactga atcctgataa accggtggtt ggcggtacag ttacggcaat ctggacggca
     2401 aaagatgcta atgacaaccc tgtaactggt ctcaatccgg atgcaccgtc attatcgggc
     2461 gcagctgctg taggttctac ggcatcaggc tggacggata atggcgacgg gacctggact
     2521 gcgcagattt ctctcggcac tacggcgggt gaattagagg ttatgccgaa gctaaatgga
     2581 caggatgcgg cagcgaatgc ggctaaagta accgtggtgg ctgatgcgtt atcttcaggc
     2641 cagtcgacag tgtctgtagc cgcagatcgc gtaaaagcag gcgaaagcac aaccgtgacg
     2701 cttattgcaa aagatgcaca tggcaacgct atcagtggtc tttccctgtc ggcaagcctg
     2761 acgggtgctg cgtctgaagg ggcgactgtt tctggttgga ccgaaaaagg tgatggttcc
     2821 tatgtcgcta cgctgacaac aggtggaaag acgggtgagc ttctcgtcat gccgctattc
     2881 aacggccagc cagcagccac cgaagccgcg cagttgactg tcattgcggg ggagatgtca
     2941 tcagcgaact ctacgcttgt tgctgacaat aaggctccga ccgtcaaaac gacgacgaaa
     3001 ctcaccttca ccgtgaagga tgcgtacggg aaccttgtca ccgggctgaa gccagatgca
     3061 ccgcagttta gtggtgccgc cagcacgggg acagagcgac cttcaacagg agactggaca
     3121 gaaacaagta atggggtcta cgtggcgacc ttaacgctgg gatctgccgc gggccagttg
     3181 tctgtgatgc cgcgagtgaa cggccaaaat gccgttgctc agccactggt gctgaatgtt
     3241 gctggtgacg catctaaggc tgagattcgt gatatgacgg tgaaggttga taaccagctg
     3301 gctaatggac aatcgactaa ccaggtaacc ctgaccgttg tggacaccta tggtaacccg
     3361 ttgcagggac aaaatgtgac gctgactctg ccgaaaggtg tgaccagcaa gacggggaat
     3421 acggtaacaa ccgatgcggc aggtaaagcc gacattgagc tgatgtcaac ggttgccggg
     3481 gaacacagca tcacggcctc agtgaataat gctcagaaga cggtcacggt gaaattcaac
     3541 gcggatgcca gcaccggtca ggcaaacctg caggtagaca ccgctgctca aaaagtggca
     3601 aacggcaaag atgcctttac gctgacggcg aacgttgagg ataaaaatgg taaccctgtt
     3661 ccagggagcc tggtgacctt taatctgccc cggggtgtca agccgcttac aggcgataat
     3721 gtctgggtga aagccaacga tgaggggaaa gccgagttgc aggtggtttc agtgactgcc
     3781 ggaacgtatg agatcacggc atcggcaggg aatagccagc cttcgaatac gcagactata
     3841 acgtttgtag ccgataaggc taccgcaacc gtctccggta ttgaggtgat gggcaactat
     3901 gcgctggcgg acggcaaagc caaacaaacg tataaagtta cggtgactga tgccaataac
     3961 aatttggtga aagatagcga agtgacgctg actgccagcc cggcttcgtt aaacctggaa
     4021 ccgaatggca ctgcgacaac gaatgagcaa gggcaggcta ttttcaccgc taccactact
     4081 gttgcggcga catacacact caaggcgcaa gtgagtcaga ccaacggtca ggtatcaacg
     4141 aaaactgccg aatctaaatt cgttgcggat gataaaaacg cggtactcac cgcatcatct
     4201 gatatgcaat ctctggtggc ggatgggaaa tcgactgcga agctggaggt gacactgatg
     4261 tcggcaaaca accccgttgg cgggaatatg tgggtcgaca ttcagacgcc ggaaggggtg
     4321 acggaaaagg attatcagtt cctgccgtcg aaaaatgacc atttcgtgag cggaaaaatc
     4381 acgcgtaaat ttagtaccag caagcctggt gtctatacgt tcacatttaa cgccctgacc
     4441 tatggcgggt acgaaatgaa gccagtgacg gtgaccatta ccgcggtgga tgccgatacg
     4501 gcaaaggacg aggaggcgat gaaataattt taagaatatg aaagtaacat tctaataaac
     4561 a
//
LOCUS       DAZGJW010000255         4519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZGJW010000255 DAZGJW010000000
VERSION     DAZGJW010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.257"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            245..424
                     /gene="yciG"
                     /locus_tag="ACHKIK_004903"
     CDS             245..424
                     /gene="yciG"
                     /locus_tag="ACHKIK_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO9085900.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            510..1010
                     /gene="yciF"
                     /locus_tag="ACHKIK_004904"
     CDS             510..1010
                     /gene="yciF"
                     /locus_tag="ACHKIK_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO9085901.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            1056..1562
                     /gene="yciE"
                     /locus_tag="ACHKIK_004905"
     CDS             1056..1562
                     /gene="yciE"
                     /locus_tag="ACHKIK_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO9085902.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1622..1747)
                     /locus_tag="ACHKIK_004906"
                     /pseudo
     CDS             complement(1622..1747)
                     /locus_tag="ACHKIK_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            1971..2066
                     /locus_tag="ACHKIK_004907"
                     /pseudo
     CDS             1971..2066
                     /locus_tag="ACHKIK_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2292..2540
                     /locus_tag="ACHKIK_004908"
     CDS             2292..2540
                     /locus_tag="ACHKIK_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO9085903.1"
                     /translation="MRVEICIAKEKISKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2537..2623)
                     /locus_tag="ACHKIK_004909"
                     /pseudo
     CDS             complement(2537..2623)
                     /locus_tag="ACHKIK_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            2708..2791
                     /locus_tag="ACHKIK_004910"
                     /pseudo
     CDS             2708..2791
                     /locus_tag="ACHKIK_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            complement(2912..3925)
                     /gene="nleC"
                     /locus_tag="ACHKIK_004911"
     CDS             complement(2912..3925)
                     /gene="nleC"
                     /locus_tag="ACHKIK_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFO9085904.1"
                     /translation="MYKTILSINMKIPVLQPGFNFFAPAGYSAAVAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVISAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNEEVHIGCIKYRNEYELNGESAIKIDDIQSLTCNELYGYDVGQEPILPICEA
                     GENENEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGASDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYADPEREAHLRLRNLNALRQAAMRHEKNERAFFERLGTISD
                     RYEASPDFTEYSAVSNIGYGFIQQHDFPGVAIDENLQDVNQIQLYHGAPYIFTFGDVD
                     RHN"
     gene            complement(4328..>4519)
                     /locus_tag="ACHKIK_004912"
     CDS             complement(4328..>4519)
                     /locus_tag="ACHKIK_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO9085905.1"
                     /translation="TAQLRLGPADILESDENGIIPEQDRVITQVVILDADKKQIQCVV
                     RPLQILRADGTWENIGGMK"
BASE COUNT         1360 a         1013 c          905 g         1241 t
ORIGIN      
        1 ccaacagtaa tataatcaaa caaattaatc ccgcaacata acaccagtaa aatcaataat
       61 tttctctaag tcacttattc ctcaggtaat tgttaatata tccagaatgt tcctcaaaat
      121 atattttccc tctatcttct cgttgcgctt aatttgacta attctcatta gcgactaatt
      181 ttaatgagtg tcgacacaca acactcatat taatgaaaca atgcaacgca acgggagaaa
      241 taacatggcc gaacatcgtg gtggttcagg aaatttcgcc gaagaccgtg agaaggcatc
      301 cgacgcaggc cgtaaaggcg gtcagcatag cggcggtaat tttaaaaatg atccgcaacg
      361 cgcatctgaa gcgggtaaaa aaggcggtca acaaagcggt ggtaataaat caggcaaatc
      421 ctgattcacc gttaattatt aatatcagct gataaagata attgcatctg cgggccgtat
      481 ggctcgcagt gatttcactg gagaaaaata tgaatatgaa gaccattgaa gatgtattta
      541 ttcacctgct ttcagatacc tacagcgcag aaaaacaatt aacccgggca ctggcaaaac
      601 tcgcaagagc aacatcaaat gaaaaattaa gtcaggcttt tcatgcgcac ctcgaggaaa
      661 ctcatggaca gattgaacgt attgatcaag ttgtggagtc cgaatcgaac ctgaaaatta
      721 agcgcatgaa atgtgtggca atggaaggtc ttattgaaga agctaatgag gtcatcgaaa
      781 gtaccgagaa aaacgaagtg cgtgatgccg cactgattgc cgcagcgcag aaagtcgagc
      841 attatgagat tgccagttac gggacattag cgacgctggc tgaacaatta ggttaccgta
      901 aagcagcgaa gcttctgaaa gaaaccctgg aagaagaaaa ggccaccgac atcaaactga
      961 ctgatctggc ccttaataac gtaaataaga aagccgaaaa taaagcctga aatatgaatt
     1021 ttaactttta gtcattttat aaagaggaca ttttcatgaa tcgtattgaa cattatcatg
     1081 actggttacg tgacgcccac gcaatggaaa agcaagccga atctatgctt gaatccatgg
     1141 ccagccgtat agataattat cctgaactac gcgctcgtat tgaacaacat cttagtgaaa
     1201 ccaaaaacca gattgttcaa ctggaaacta ttcttgatcg taatgacatt tcacgttcag
     1261 tcattaaaga ttccatgagt aaaatggctg cgcttgggca gtcaatcggt ggtatattcc
     1321 cttctgatga aatagttaaa ggctctatta gcggatatgt cttcgagcaa tttgaaatcg
     1381 cctgttacac ctcactatta gcagcagcaa aaaatgccgg tgatacagtc tcaattccga
     1441 tcatcgaagc gattttaaat gaggaaaagc aaatggccga ctggctgatt cagcatattc
     1501 cgcaaacaac tgagaaattt ttaattcgtt ctgaaactga tggcgtagaa gcgaagaaat
     1561 aatacccttt ataccatgtc cttattgacc ccgtatatta cggggtcgtt tttgtgcgga
     1621 attaaaaacg atatcctgct gagaacataa acacccacgg atcgaggcgt acgctatcgt
     1681 gttgctgtgc accgcctagc ttataattgg cggtggtatc gatatccatg taccacactg
     1741 gtagatttta caaaactcaa taacatatta tatttaaagg aaaaatacag aatttacgat
     1801 gcttttttat acagatctct cgtgttttta actgattgat tttgttgaac tctttttttg
     1861 ttttgcagga gttactcctc ttaccttatt gtccactcga tgaccaacac attcacaacc
     1921 caaaccagaa tatcctatct atgaagcatg tatccgcacg cagattaatt atggtttaca
     1981 ttgttctacc acaatagtcg catcctaaat gggtgcggtt gaggatcacc atccttgaag
     2041 aggatgcccg ccactttaac tgcatgacgc aacattttta aaaatcgacg aagatcgtcc
     2101 aggagcgccg tttttcaagg gttggataga tgattcgttt accatgtcga cgaacttcga
     2161 cgaatttcgc cgataaaaag tgtcccatac atgccccatt gatgccccat aaacaatatc
     2221 aggaattgac actggttatc catacagata aaaataacac ctgtatataa ccacagttac
     2281 aggagggtgt tatgcgtgtt gagatctgta ttgctaaaga aaaaatcagc aaaatgccaa
     2341 acggtgctgt ggatgcgtta aaggaagaat taacccgacg catcagtaaa cgttatgacg
     2401 atgtagaggt gatcgtaaaa gccaccagca acgatgggct ttctgttaca cgcaccgcag
     2461 ataaagattc tgcaaaaaca tttgttcagg agactctgaa agatacctgg gagtctgctg
     2521 acgagtggtt tgttcactaa taaacacgta aaatcggtaa cggctggaaa tcattcaata
     2581 ctcgcactat cgaaagttca ccagccaacc gcgacacgtt cttacatacg aagtgccgcg
     2641 ctttccttaa taatttttta gcagtactgt gtaaatgatg agcgacctaa tccatgcatg
     2701 acgcgtagtg cctattgtgc atctttctgc gtctcttttt actggaccca agccaatgat
     2761 gctggcgata ccattcccgc caggcccact tacaacagta aaattcgggg ctctgccttt
     2821 acatacaata caatgttgca cgattgccca tcatcaactc cgttaatatc tatgagtagt
     2881 aaccctatgt aactgtaaag acgaatcagc gctaattgtg tctgtccaca tccccaaatg
     2941 taaagatata aggagcacca tgatacagtt ggatctgatt tacatcctgt aaattctcgt
     3001 cgatagccac ccctggaaaa tcatgttgct ggataaatcc gtatcctatg ttagacacag
     3061 cggaatactc tgtgaaatca ggactcgcct catatcggtc actgatcgta cccagtcttt
     3121 cgaagaaagc cctctcattc ttttcatgcc tcatggcagc ctgtcgaagg gcattcaggt
     3181 tacgtagtct aagatgagct tctcgttctg gatctgcata tcctttgaag tcgggaacac
     3241 tccatcccag ctcttgagcg acacgacgtg cgagaatctc ggtgggtcct agctctatat
     3301 tactatctcc agatggatcg ctggccccag taacatgatg aataatctcg tgaatcagtc
     3361 cttcctgcca cgatggcatc tcataagaat cagtatctgg cgcaacacta aaactgacat
     3421 aaggctcttc gttctcgttt tctcctgcct cgcaaatggg gagaattggt tcttgcccga
     3481 catcgtatcc atataattcg ttacaggtta gtgattgaat atcatcaatt ttgatagcag
     3541 attctccgtt aagctcgtat tcgtttctgt atttaatgca accaatgtgt acctcctcat
     3601 tatgaatgcc atagcttacg gcatcacgaa atgtttgtga tcggctcaac gcatcaagta
     3661 cagtgttgcc aatcatatcg accgtatgct gatcgatgag cctgctacgg ctgtcatgga
     3721 cggcgctaat tacactttcg tatacgttgc ttaaatctgc tgcggaaagc ggtattcgct
     3781 tgcctatatc caaaacgtaa tccgcatagg cattttcagc acgattagga gcaacggcag
     3841 cagagtatcc agcaggggca aaaaagttga agccaggttg gagaacggga attttcatat
     3901 tgatacttaa tatggtttta tatatttcag tgccaccaat tcactagctt acaaccagaa
     3961 actaaaaaaa cgtcagggcg ttgataataa tgaataccaa ctttctgccc atttatagta
     4021 atatctttcg ttatcaggtt ttgtctggca gcttcacgta atatttcaat actctgcaaa
     4081 gcaacatttg atgaaatgct ggatgttatc aatggcatat gtcatttacc ccaacaagac
     4141 taaattttag aaaaaacaac tgccgggaca actttcgccc cctgtaaccc caaataagat
     4201 gtcagattta tcggcattaa tttatctccc tgttgaatta gctcctctat catctactta
     4261 acaaatatcc cccggacatt gcaacacaaa aaccggagcc ggactccggt tttgtgaagc
     4321 tgtcgggtta cttcatcccg ccaatatttt cccacgtccc gtcagcacgc aggatttgca
     4381 gcggtcttac cacgcactgt atctgctttt tatccgcatc cagtatcacc acctgcgtga
     4441 ttaccctgtc ctgctccggg ataatgccat tctcatctga ctccaggatg tctgccggtc
     4501 ccagtcgcag ctgtgctgt
//
LOCUS       DAZGJW010000256         4504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZGJW010000256 DAZGJW010000000
VERSION     DAZGJW010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.258"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..4295
                     /locus_tag="ACHKIK_004913"
     CDS             <1..4295
                     /locus_tag="ACHKIK_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease fold toxin"
                     /protein_id="HFO9085906.1"
                     /translation="PQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTESNAGFM
                     DRVSAGASLLFPAGPVLMEFATMVGGRSEIKKDVDFPEAGEDTALCDKENKPPRIAQG
                     SSNVFINNQPAARKGDKLECSAAIVGGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGI
                     TIASYLLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKV
                     IRWVTDPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYA
                     WVREQGNRVDIISQGATLNFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRF
                     FYDAFPGMRLRHPVTDDTSDDRLAHSPDDRMYMLGGMSDTASNRITFERDSQYRITGV
                     SHTDGIRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYD
                     AADRIIRWSDNDQTWSRFTYDAQGRCVNVTGAEGYYNATLDYGDGCTTVTDGKGTHRY
                     YYDPDGNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAALGQLSRYTAAD
                     GAEWLYRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSG
                     IFRQDERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQH
                     HKLSGLERAETGAEGFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQY
                     RYDKDTLQLTEVINPQGESYRYILDNCGRVTEERDWGGVVWRYRYDADGLCTARVNGL
                     EETILYSRDAAGRLAEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVT
                     TQGRRAIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDE
                     TLTFSRDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGFFPEETTWGGLLPSLVREY
                     RYDSAGNVSAVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCG
                     YLKAQSAGRHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERF
                     RWDSRDQLTGYCSAQGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRD
                     DKLYSVRHLVFNGFELISQQFSRVRQAHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEG
                     KTVWRPGQTSLWGLALSLPADTGYPDPRGEWDPEADPGLLYAGQWQDAESGLCYNRFR
                     YYEPETGMYLVSDPLGLQGGEQTYRYVPNPLGYIDPLGLAKTSVPAEKISLSDKARDL
                     FRQGKVREALDVHYEDLVRRKLGGISQEIAGREYDVVTDKIIAQVKRTYSSIDNPKNF
                     LSKSTRTQIKKTIELAEEQGKEAQFWFKYGVSPKVREYIESKGGKVILGMGN"
     gene            4297..>4504
                     /locus_tag="ACHKIK_004914"
     CDS             4297..>4504
                     /locus_tag="ACHKIK_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004014665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085907.1"
                     /translation="MSRYILIRGWFECDFEQVKKIKEANEEFFKHSEKYLLPENTKRL
                     YLNGWCYPEYPINWVSVISLGVNIN"
BASE COUNT         1100 a         1194 c         1396 g          814 t
ORIGIN      
        1 tcccacaggg ggaaccctca tggggtgata ttgccactga cctgcttgaa tcggcagcgg
       61 aaaaagcagt accactggcg aaggcctggg ggaacgctgt tatcaccctg acggaaagca
      121 atgccggttt tatggatcgc gtcagcgccg gcgcatcgct tctgtttccc gccggtccgg
      181 tattaatgga gtttgccacc atggtgggcg ggcgtagcga aatcaaaaaa gatgtggatt
      241 tcccggaagc cggtgaggac acggcgctct gcgacaagga gaacaaacca ccgaggatag
      301 cccagggcag tagcaacgtc tttatcaaca atcagcctgc cgcgcgcaag ggcgacaaac
      361 tggagtgcag tgcggcgatc gtgggaggtt cgccggacgt ctttattggc ggtgaacagg
      421 tgacctatct ggatatccag ccggagttcc cgccgtggca gagaatgatc ctggggggaa
      481 taacgatagc cagctatctt ctgccgcctg cgggactgct gggaaaactg aagaatctgg
      541 cgagactcgg caaactggga aacctgctgg ggaaaagcgg gaagctgctg ggcgcaaagc
      601 tcggcgcgtt gctggggaaa acaggtaagt cgttaaaaag tattgccaat aaagtcatca
      661 gatgggtaac agatcctgtc gatccggtaa ccggcgcata ctgcgacgaa cgtaccgact
      721 tcaccctggg ccagaccctc cccctctcct tcacccgttt ccacagttct gtactgccgc
      781 tgcatggcct gaccggcgtg ggctggagcg actcctggag cgaatacgcc tgggtgcgtg
      841 aacagggaaa tcgggtagat attatcagcc agggagccac gctgaacttc gccttcgacg
      901 gtgacagtga tacggcggtt aacccgtatc acgcccagta cattctgcgc cgccgcgacg
      961 attacctgga gctgttcgac agggatgcac tgagcagccg cttcttttat gacgcctttc
     1021 cgggaatgcg tctgcgccac ccggtgactg acgataccag cgatgaccgc ctggcacaca
     1081 gccccgacga ccggatgtat atgctgggcg ggatgagcga caccgccagc aaccgcatca
     1141 cgtttgagcg cgacagccag taccggatca cgggtgtcag tcacaccgac gggatccggc
     1201 ttaaactgac gtaccacgcc agcggctacc tgaaagccat tcaccgcacg gataacggca
     1261 tacagacgct ggcgacctac gaacaggatg cgcgggggcg gctgacagaa gcggatgcgc
     1321 ggctggacta ccacctgttt tatgagtacg acgctgcgga ccggatcatc cgctggtccg
     1381 ataacgacca gacgtggagc cgtttcacct acgatgcaca gggccggtgc gtgaatgtca
     1441 ccggggcgga gggctattac aacgccacgc tggactatgg tgacggctgc accaccgtga
     1501 cggacggcaa gggcactcac cgttattact atgatcctga cggcaatatt ctgcgggaag
     1561 aagcgccgga cggcagtacc accacgtatg aatgggatga attccatcac ctgctggccc
     1621 gccactcccc tgccgggcgg gtggagaagt ttgaatacaa cgccgcactc ggtcagttaa
     1681 gccgttacac ggcggcagac ggcgcggagt ggctgtaccg ctatgatgag cgcggcctgc
     1741 tcagcaacat caccgaccct gccgggcaga catggacgca gcagtgtgat gagcgcggcc
     1801 tgccggtaag cctggtgtcg ccacagggcg aagagacccg gctggcatac accgctcagg
     1861 ggctgctgtc ggggatattc cgccaggatg aacggcgtct gggcatagag tacgaccacc
     1921 acaaccggcc ggaaacactc accgacgtga tgggccgtga acaccacacc gaatacagcg
     1981 gtcacgacct gccggtgaag atgcgcggcc ccggcggtca gtcagtgcgg ttacagtggc
     2041 agcagcacca taaactgagc ggccttgagc gggcagaaac cggcgcagaa ggattccgct
     2101 atgaccgcca cggcaacctg ctggcgtaca cggacggtaa cggcgttgtc tggacaatgg
     2161 aatacggccc gttcgatttg ccggtggcgc gaacggacgg tgaaggccac cgctggcagt
     2221 accgctacga taaagacacg ctgcagctca cagaagtcat taacccgcag ggcgagtcat
     2281 accgttatat tctggacaac tgtggccggg tgacggaaga gcgtgactgg ggcggcgtgg
     2341 tctggcgtta ccgctatgac gctgatggcc tgtgtaccgc cagggtcaac ggcctggagg
     2401 aaaccatcct ctacagccgg gacgccgcag gccgcctggc agaagtcatc acgccggaag
     2461 gcaaaacgca gtatgcgtat gacaaatccg gcaggctgac gggtatcttc agcccggacg
     2521 gcacatcaca gcgcaccggc tatgacgaac gcgggcgggt gaatgtcacc actcagggcc
     2581 gacgggccat tgaataccac taccccgatg aacacaccgt tatccgctgt atcctgccac
     2641 cggaagatga acgcgacaga caccccgatg aatccctgct gaaaaccacg taccgctaca
     2701 acgccgccgg agaactgaca gaggtcattc tgccggggga tgagacgctg acgttcagcc
     2761 gtgatgaggc gggacgtgaa gtgctccggc acagtaaccg gggttttgcc tgtgaacagg
     2821 gctggaatgc agccggccag cctgtcagtc agcgcgccgg attttttccg gaggaaacca
     2881 catggggcgg gctgctcccc tcactggtac gggagtaccg ttacgacagc gcgggcaatg
     2941 tgtcggctgt caccagccgg gaagattacg gacgggaaac ccggcgggag taccggctgg
     3001 accggaacgg ccaggtcacg gcggtgacag cctcaggcac cgggctgggc tatggcgaag
     3061 gcgatgagtc ctatggctat gacagttgtg gctacctgaa ggcgcagtct gcgggcaggc
     3121 accggataag tgaagagact gaccagtatg ccggaggcca ccggctgaaa caggccggaa
     3181 acacgcagta tgactatgac gccgcaggcc ggatggtcag ccgcaccaga caccgtgacg
     3241 gctaccgccc ggaaacagag cggttccggt gggacagccg ggaccagctg accgggtatt
     3301 gcagcgcaca gggtgagcag tgggaatacc gccacgacgc cagcggcaga cgaacagaga
     3361 aacgctgcga ccggaagaaa atccgcttca cgtacctgtg ggacggcgac agtattgcgg
     3421 aaatccggga ataccgcgat gataaactgt acagcgtacg gcacctggtg tttaacggct
     3481 ttgagctgat aagccagcag ttcagccggg tacggcaggc gcatccgtcg gtggccccgc
     3541 agtgggtgac gcggacgaat catgcggtga gcgacctgac gggccgcccg ctgatgctct
     3601 ttaacagtga aggtaaaacc gtctggcggc cgggacagac cagcctgtgg gggctggcac
     3661 tcagcctgcc cgcagacacc ggctacccgg acccgcgcgg ggagtgggac ccggaagccg
     3721 accccggcct gctgtatgcg ggacagtggc aggatgcgga atcggggctg tgctataacc
     3781 ggttccggta ctacgagccg gaaaccggaa tgtacctggt gagtgatccg ctggggttgc
     3841 aggggggtga gcagacttac cggtatgtgc ctaatccact ggggtatatt gatccgctgg
     3901 ggctggcaaa aacttctgtt cccgcagaaa aaataagcct ttcggataaa gcaagagatc
     3961 tttttagaca aggaaaagta agagaagcat tagatgttca ttatgaggat cttgtacgaa
     4021 gaaagcttgg tggtatatct caagaaattg ccgggcgtga atatgatgtt gttactgaca
     4081 agatcatagc gcaggtcaag aggacttata gctctataga taatccaaaa aacttcttaa
     4141 gtaagtctac tcggacacaa attaagaaga caattgagct ggctgaagaa caggggaaag
     4201 aggctcagtt ttggtttaaa tatggagtaa gccctaaagt aagagaatat atagaaagca
     4261 aaggtgggaa agttatattg ggtatgggga attaacatgt caagatatat acttattaga
     4321 ggttggtttg agtgcgactt tgaacaagta aaaaaaataa aagaagctaa tgaagaattt
     4381 ttcaaacatt ctgaaaaata tttattacca gaaaacacga aacgacttta tttaaatggg
     4441 tggtgttacc cagaatatcc tatcaactgg gttagcgtta tttccttagg tgttaatata
     4501 aatc
//
LOCUS       DAZGJW010000257         4490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZGJW010000257 DAZGJW010000000
VERSION     DAZGJW010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.259"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(113..802)
                     /locus_tag="ACHKIK_004915"
     CDS             complement(113..802)
                     /locus_tag="ACHKIK_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO9085908.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(817..939)
                     /locus_tag="ACHKIK_004916"
     CDS             complement(817..939)
                     /locus_tag="ACHKIK_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO9085909.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
     gene            1278..2237
                     /locus_tag="ACHKIK_004917"
     CDS             1278..2237
                     /locus_tag="ACHKIK_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFO9085910.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
     gene            complement(2381..2452)
                     /locus_tag="ACHKIK_004918"
                     /pseudo
     CDS             complement(2381..2452)
                     /locus_tag="ACHKIK_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
     gene            complement(2449..2637)
                     /locus_tag="ACHKIK_004919"
     CDS             complement(2449..2637)
                     /locus_tag="ACHKIK_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFO9085911.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            complement(2634..2996)
                     /locus_tag="ACHKIK_004920"
     CDS             complement(2634..2996)
                     /locus_tag="ACHKIK_004920"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9085912.1"
                     /translation="MNEYQFVLPYPPSVNTYWRRRGSQYYISDKGQKYRKDVQQIIHQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENA"
     gene            complement(2993..3283)
                     /locus_tag="ACHKIK_004921"
     CDS             complement(2993..3283)
                     /locus_tag="ACHKIK_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO9085913.1"
                     /translation="MANLRKESRGRECQVRIYGICNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            complement(3283..4005)
                     /locus_tag="ACHKIK_004922"
     CDS             complement(3283..4005)
                     /locus_tag="ACHKIK_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO9085914.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEVSERINNLGFNVQYEHYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEETAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(3998..4207)
                     /locus_tag="ACHKIK_004923"
     CDS             complement(3998..4207)
                     /locus_tag="ACHKIK_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO9085915.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEYCCAELMSDPNSSMYEEEDDE"
     gene            complement(4167..>4490)
                     /locus_tag="ACHKIK_004924"
     CDS             complement(4167..>4490)
                     /locus_tag="ACHKIK_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085916.1"
                     /translation="MVSIRLPFISIYWLNREATNYWYTCARAAFNDPDWFVKNHHAVR
                     QAKRKANMTYMKAYQKAWKEHRDRYQQDMEKLESENMELRRKLGEAKRDIDAYKRLFN
                     GESHA"
BASE COUNT         1222 a         1045 c          906 g         1317 t
ORIGIN      
        1 gcagccagca tgtttaccgt acaagtacaa ctcagggcat aaaaaaaacc cactcggcag
       61 cgggtttata cattttttac aacataccaa atttgcatga agtatatggc ttttaatcca
      121 gttttgcaat attttgctgt aaaaatgctg ccttttgttt tgaacgtgtt ctcgtcacaa
      181 gcaataaagc atcactatca agctgtagaa aaatgtgctt cattgcaacc cagcgttcag
      241 taaatgtctc ggaccagttt tttgttgtca ctcccaccaa tgatgccagt gtctggtatt
      301 cataggcctc acgccctgca agttcgctct tcacatcctg tgcagccagc cagattaacg
      361 tcttcaggcg atccagtgtc ttacctgcaa tttttctgtt acttaacaaa tctttaaact
      421 cgctccatgc ccattgcgtt atggtgacct gatgccccca tcgaacgctt tcgctgtaac
      481 accaaagcaa ccatgctttc tgatgttcat cgagagacaa aaccgcgcgg cgccatgaag
      541 aggttgagaa ttcaaccggg ctgaccaaag caatggatga accttttgcg tacgactgct
      601 taccggaagt cggcgtatta tccagcgtaa tcatcttgcc agttaccaca tccagaatgc
      661 gcggcttctt tcgtttgtat gtaccagtat caaattgtgc atgctcctgc caggcttcga
      721 gctggccttt cgttgctccg ttcaagtcag cagtagctgc cataagttgc tcacgaacat
      781 actgtaaata ttgggtattc atgcagtaaa tcctttctat attttggcat aattcttcaa
      841 cattcggtaa tcgttcaaaa ccgaatcggg gaaacgacat aagcacagga gcccccagcg
      901 acagcgaagg agttctgata tataagactc agacatcatt cattccccgg ttctccaata
      961 tctgtttcac tcatcatcca taactacctg taattgcccc cccttttttt gtaacagttc
     1021 ttatattgct atatagaata gccattacta atgcttttaa atttaataaa ataaaaatta
     1081 taaaaaacat aaaacaccac gcaaacacac ttaataaaaa caccgttaca ttaaaagata
     1141 ataaaaaccg caataaaaaa cgaataaatc aattgtctca cgcaattata aaacatcata
     1201 ttgattacgc accttgtatt acaaactcat gtatgtaaaa tacgcgcacc attcaaaaaa
     1261 aaggaagaca ataacatatg aaaaaaagtg tcatcgctgg cgtctttatt gctctgtcat
     1321 ttaccacgtg ttcagctatc gcgaacagcc ttgcattatc attagcaaat gatgatgcag
     1381 ggaagtttca accaatactt aatgatattt atggcaataa acatgaaaac agagatgatt
     1441 actcacaagg cttatttctg ggatatagcc acgatatctc agactcgagc caattatctc
     1501 tccatattgc gcaagatatt tactctccat caggcagtaa taaaagacac aacacagctg
     1561 taactggaga cagagctttt agtgcataca ctcacactgg tattgaatgg aactcccttg
     1621 cgaatgactg gattcgctat cgattaggta ctgacatagg tgttgttggc cccgacgcag
     1681 gcggtcagaa agtacaaaat aaagctcatg agattattgg ggcagaaaaa tatcatgcat
     1741 gggatgatca aatagagaat cgctacggtt atactgtaaa agggatgcta tccatgacac
     1801 caagtatgga tattttaggt gctaatgttg gattataccc tgaagtttct gctgttactg
     1861 gaaacttatt tcaatatgta gcatatggcg caaccattgc cattggtaat gataaaacct
     1921 tcaattcgga taatggcttt ggtctgctgg ctccccgtgg tttaatgcat atgtccgata
     1981 caagcggatt caaatacaag atttttgcag gtatggaaag acgagatgtc aatcgcaact
     2041 atactctcga aggaaaaaca atacagacga aacaaacaac agtatcgcta aacaaaactg
     2101 ttgatgaata tcaagttggc gcaacaattg ggtatgcacc tgtagccttc acactagcat
     2161 ttaataaagt aacatcagaa ttcaagacag gggatgacta ttcatttata aatggagcaa
     2221 tcaccttctt tttttaactg aattgaattc aatcaaaata acataagtcc aacaaaaaca
     2281 taaagtgcga aatgaatgcc agctccattt atttcgcact ataaaagatt aaaagttgca
     2341 ataaaataat aaaatgactc agttacgaaa accaataaac tgtggccagt agtgagtcgc
     2401 tcatcatcgg gctttttggc gaatgaaatt tagctacgct ttcgagtctc atcgtcttcc
     2461 cctcttgccc tgtttgacca tcaggacgcc gttaactatt acatgacgct cgcctttgct
     2521 gtctcggttg tacttgagca ctgttcctct tgcgcaggaa agcatcctcg ccacttcggt
     2581 ctgattgcct cgtgtctgga taagaagctc tggtatcgtt tgaattgtgg cgttcatgcg
     2641 ttctccagtt cggtgatttt tattccaagc cgtccgcctg gtactttcac accacgaatt
     2701 acgcgaatgt catcgaattg ctcgtcgtct tccgcaaatc cggcatggat aagggagtcg
     2761 agtaaaccct tcaggatgtt atctaggtcg cggcggcggg agtctggaac gtctgcgatt
     2821 actttgatgc ggagtcgtga tttggtgaaa atatctaact tgagttggtg gatgatttgc
     2881 tgaacgtctt ttcggtattt ctggccttta tcgctgatgt agtattggct tccccgtctt
     2941 cgccagtagg tattcaccga cggcgggtat ggaagcacaa actgatattc gttcatgact
     3001 taatcttccc ctccttcagc agtatcgcct gcgtcctgat cacgccttcg aggtggtaaa
     3061 gtctggcgtc tttgttgtcg aggttatggg tgcgtcggtc gatttcatcg tgacacgcgc
     3121 tacaagccca tgcgccgatc aggtcgtcag gcttcattcc cgttccgcaa attccagcca
     3181 tccggtaatg tgccagaact gtagtttcag gattgccatt gcatatgccg taaatacgta
     3241 cctggcattc tctgccgcga gattctttgc gtaggttagc cattaagcag cctcccctgt
     3301 tactttcagc attccgttat cgagcagctt tctggtcagc cactgttgac cacgcccggt
     3361 gatttttgtg gtgaacgata tctgtattcc gtgatttgtg ttgaccgctg tttctttcac
     3421 tgtgaaatag ccgcgatcca tatattcctg cattggcaca ttgcgccggg aacctgaagc
     3481 aataaggatt ttgtgatcgc gcatccacgc aaacagtttg tttggaccaa taccaacaac
     3541 ctttgcaaag tttccaatca aaattccgct ggcctcgcca acgcgatcgg caaactcaac
     3601 tttaggtgcg gcaattgcga gctggttttc cagttgcatt ttctgctcag caagatcagc
     3661 agcaaggcgc aacgcttccg gtaacgtttt ggggatatta accgcagttt cttcaagctc
     3721 tcgccaacgg tcaacaagac gagcggtgaa ctctggcgac aactgggcaa caacgacaat
     3781 actgtctcgc ttaccttgtt cgccctcgaa gacgtaatgc tcgtactgaa cattgaaccc
     3841 taagttattg attctttcgg aaacctcaat ttgaggaagc cggataacac catttttagc
     3901 cagcgtttcg atggtacgtt tcacattgtc atgacgctta cccaccaact cagctatttc
     3961 aatgcttgtc attttgatgg cattgccatt tattaactca ttcatcgtct tcttcctcgt
     4021 acattgagct attcggatcg ctcatcagtt ctgcgcagca gtactcacac acgtgaactt
     4081 ccagcacatg cagcttctga ccgcagttag cgcacgttaa agctcgctcg acgctttctt
     4141 tctggtattg aagggattgg gatgggctaa gcatggcttt caccattaaa aagtcgcttg
     4201 taagcatcaa tgtctcgttt tgcttcaccg agctttcgtc ttaattccat gttttctgat
     4261 tcaagctttt ccatgtcttg ttggtatcga tcgcggtgtt ctttccatgc tttttgatac
     4321 gccttcatgt atgtcatatt ggcctttctc tttgcctgac gaactgcgtg gtggtttttc
     4381 acaaaccagt cagggtcgtt aaatgctgct ctggcgcatg tataccaata atttgttgcc
     4441 tccctgttta gccaataaat actgataaat ggcaaccgga tagacaccat
//
LOCUS       DAZGJW010000258         4477 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZGJW010000258 DAZGJW010000000
VERSION     DAZGJW010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.260"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..98
                     /locus_tag="ACHKIK_004925"
     CDS             <1..98
                     /locus_tag="ACHKIK_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO9085917.1"
                     /translation="DDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            104..889
                     /gene="bet"
                     /locus_tag="ACHKIK_004926"
     CDS             104..889
                     /gene="bet"
                     /locus_tag="ACHKIK_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO9085918.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            886..1563
                     /locus_tag="ACHKIK_004927"
     CDS             886..1563
                     /locus_tag="ACHKIK_004927"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO9085919.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVYGEQW"
     gene            1563..1745
                     /locus_tag="ACHKIK_004928"
     CDS             1563..1745
                     /locus_tag="ACHKIK_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO9085920.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            1718..1909
                     /locus_tag="ACHKIK_004929"
     CDS             1718..1909
                     /locus_tag="ACHKIK_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO9085921.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMAAKAEANERDPA"
     gene            1986..2201
                     /locus_tag="ACHKIK_004930"
     CDS             1986..2201
                     /locus_tag="ACHKIK_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO9085922.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            2300..2521
                     /locus_tag="ACHKIK_004931"
     CDS             2300..2521
                     /locus_tag="ACHKIK_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO9085923.1"
                     /translation="MADIIDSASEIEELQRNTAIKMHRLNHQAISATHCCECGDPIDE
                     LRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            2518..2823
                     /locus_tag="ACHKIK_004932"
                     /pseudo
     CDS             2518..2823
                     /locus_tag="ACHKIK_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            3120..3470
                     /locus_tag="ACHKIK_004933"
     CDS             3120..3470
                     /locus_tag="ACHKIK_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO9085924.1"
                     /translation="MINRTKLEHILEYARQQRRFGQLCKILPGDMVEIVEIAMRKTGN
                     SPVSPGGWISCSERMPAQDDWILIYSKHGEYMAGQVQEEYVELSDGTLSWLGNALYWM
                     LLPEPPQGVNDELA"
     gene            3545..3787
                     /locus_tag="ACHKIK_004934"
     CDS             3545..3787
                     /locus_tag="ACHKIK_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9085925.1"
                     /translation="MFALIQRGQIYTDSAGYPIKILRCINNTVLYRRMDGRTQSVKIN
                     DFNELFERIDHQEYRQILAETEQENHLKKLRAMQRR"
     gene            3906..4250
                     /locus_tag="ACHKIK_004935"
     CDS             3906..4250
                     /locus_tag="ACHKIK_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085926.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            4356..>4477
                     /locus_tag="ACHKIK_004936"
     CDS             4356..>4477
                     /locus_tag="ACHKIK_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO9085927.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGRE"
BASE COUNT         1271 a         1019 c         1172 g         1015 t
ORIGIN      
        1 atgacgatgt tgagtttcag gaacgcatgg cagaacacat ccggtacatg gttgaaacca
       61 ttgctcacca ccaggttgat attgattcag aggtataaaa cggatgagta cagcactcgc
      121 aacgctggct gggaagctgg ctgaacgtgt cggcatggat tctgtcgacc cacaggaact
      181 gatcaccact cttcgccaga cggcatttaa aggtgatgcc agcgatgcgc agttcatcgc
      241 attgctgatc gtcgccaacc agtacggcct taatccgtgg acgaaagaaa tttacgcctt
      301 ccctgataag cagaacggca tcgttccggt ggtgggcgtt gatggctggt cccgcatcat
      361 caacgaaaac cagcagtttg atggcatgga ctttgagcag gacaatgaat cctgcacatg
      421 ccggatttac cgcaaggacc gtaatcatcc gatctgcgtt accgagtgga tggatgaatg
      481 ccgccgcgaa ccattcaaaa cccgcgaagg cagagaaatc acggggccgt ggcagtcgca
      541 tcccaaacgg atgttacgtc ataaagccat gattcagtgt gcccgtctcg ccttcggatt
      601 tgctggtatc tatgacaagg atgaagccga gcgcattgtc gaaaataccg catacactgc
      661 agaacgtcag ccggaacgcg acatcactcc ggttaacgat gaaaccatgc aggaaattaa
      721 cactctgctg atcgccctgg ataaaacatg ggatgacgac ttattgccgc tctgttccca
      781 gatatttcgc cgcgacattc gtgcatcgtc agaactgaca caggccgaag cagtaaaagc
      841 tcttggattc ctgaaacaaa aagccactga acagaaggtg gcagcatgac accggacatt
      901 atcctgcagc gtactgggat cgacgtgaga gctgtcgaac agggagatga tgcgtggcac
      961 aaattacggc tcggcgtcat cacagcttca gaaattcaca acgtaatagc aaaaccccga
     1021 tcaggaaaga agtggcctga catgaaaatg tcctacttcc acaccctgct ggctgaggtt
     1081 tgcaccggtg tggctccgga agttaacgct aaggctctgg cctggggaaa acagtacgag
     1141 aacgacgcca gaaccctctt tgagttcact tccggcgtga atgttactga atccccgatc
     1201 atctatcgcg acgaaagtat gcgcaccgcc tgctctcccg atggtttatg cagtgacggc
     1261 aacggccttg agctgaaatg cccgtttacc tcccgggatt tcatgaagtt ccggctcggt
     1321 ggtttcgagg ccataaaatc ggcttacatg gcccaggtgc agtacagcat gtgggtgaca
     1381 cgaaaagatg cctggtactt tgccaactat gacccgcgca tgaagcgtga aggcctgcat
     1441 tatgtcgtga ttgagcggaa tgaaaagtac atggcgagtt ttgacgagat ggtgccggag
     1501 ttcatcgaaa aaatggacga ggcactggct gaaattggtt ttgtatatgg ggagcaatgg
     1561 taatgaagca tcctcacgat aatatccggg taggcacgat cactttcgtc tactccgtta
     1621 caaagcgagg ctgggtattt cccggccttt ctgttatccg aaatccactg aaagcacagc
     1681 ggctggctga agagataaat aataaacgag gggctgtatg cacaaagcat ctcctgttga
     1741 attaagaacg agtatcggga tggcacatag cctcgctcaa attggagtca ggtttgtgcc
     1801 aataccagta gaaacagacg aagaatttca tacgttagcc acatcccttt cacaaaagct
     1861 ggaaatgatg gcggcgaaag cagaagcaaa cgagagagac ccggcatgac aacaacagaa
     1921 tgcatttttc tggcagcagg cttcatattc tgtgtgctta tgcttgccga catgggactt
     1981 gttcaatgac acctcagcag gaaaacgccc ttcgcagcat tgcccgtcag gctaattctg
     2041 aaatcaaaaa agccagacag cagtttccgg ataaaaacgt cgatgacatt tgccgtagcg
     2101 tactgaagaa gcaccgcgaa acggtaacgc taatgggatt cacaccgact catttaagtc
     2161 tggcgatcgg catgttaaac ggcgtcttta aggagcgatg aacatgaaaa gcaaaatcat
     2221 cagggagcta caggctcctt ttttattgtt cgcattcacc ctcaagcgta ttaaccaaca
     2281 attcagggat taatggaaga tggcagacat cattgattca gcatcagaaa ttgaagaatt
     2341 acagcgcaac acagcaataa aaatgcaccg cctgaaccac caggctatat ctgccactca
     2401 ttgttgtgag tgtggcgatc cgatagatga actaagacgt ctggccgttc agggttgtcg
     2461 gacttgtgca agttgccaag aggatctgga gcttatcagt aaacagagag gttcgaagtg
     2521 agcgaaatta actctcaggc actgcgtgaa gcggcagagc aggcaatgca tgacgactgg
     2581 ggatttgatg cggacctttt ccatgagctg gtaacaccat cgattgtgct ggcactgctg
     2641 gatgaacggg aaagaaacca gcaatacatc aaacgccgcg accaggagaa cgaggaaatt
     2701 gcgctaacgg tagggaagct gcgtgttgag cttgaggaag caaaatcaaa actcaacgag
     2761 cagcgtgagt attacgaagg tgttatctcg gatgggagta aacgtattgc tgaactggag
     2821 aaaagcgaag agcaactcat taacgagcgt gaccatgctg agtctgcttt agctgatatg
     2881 tacttcgcaa caaccgggga taggcctgag tggagtaact gtttcagttt ttcagatgct
     2941 gtcgatgccg tagttgacag aattgctgat ttagaagcca aacagccatc gccagtagta
     3001 ccggaagaaa aaccaatgcc taaccctctt agcatgtaca cagttgatgc tgttgcagct
     3061 attgcagagg tgagaggctg gaacgcctgc cgtgcagcca tgcttaaggg agataaatca
     3121 tgattaatcg aaccaaactg gagcacatcc tcgagtatgc caggcagcag aggcgctttg
     3181 gccagctttg taaaattctg ccaggagata tggttgaaat cgtggagatt gccatgcgta
     3241 agactggcaa ctctccggta agtccgggtg gttggataag ctgtagtgag cgaatgcccg
     3301 ctcaagatga ttggatttta atttattcaa agcacggtga gtatatggca ggacaggtac
     3361 aagaggaata cgtggagttg agcgacggca ctttatcgtg gttagggaac gccttgtact
     3421 ggatgctgct accagaaccg ccgcagggag tgaatgatga attggcctga agcattcacc
     3481 gctgtaggag ttgcaatcgc ggtggcattt attctgtatt cgcttttccg ctggggataa
     3541 aggaatgttc gctctgattc aacgtggtca gatatacacc gatagcgccg gctacccgat
     3601 aaaaattctt cgctgcataa acaacactgt gttgtacaga agaatggatg ggcgaacaca
     3661 gtcggtaaaa ataaacgatt ttaatgaact gtttgaacgg atcgatcacc aggaataccg
     3721 acaaattctg gctgaaacag agcaggagaa ccatctgaaa aaattacgcg ccatgcaaag
     3781 gagataaacc ggtaaaggtg ttcgcgataa aggtgaatat cggcaatgaa taacaatcct
     3841 cgcactcgcg gggatttctt ttatctgaac tcgctacggc gggttttgtt ttatggagac
     3901 aagaaatgtc agatttggct atgaaggttt tgaaatggca atcgactggc gatgttggca
     3961 tcagtagcgc aactcttgcc tcaatcgcat gtggactgaa aaagaatatc tatggtcatc
     4021 acttcggcgc tccacatgac gcagcagact tccggcgatg cgttgcactt gttgagcaga
     4081 ttccagaaat cagagattca ttcgacaagg ttgcaaagcg cgttccggca ttcaaaggca
     4141 tcctcaacga atgggattcc ctcgtcgctc tgttgaagtc tgaaatgaag atacacggaa
     4201 acaaagcacc agagacttac agaagaatta gcgagctacg caaggactaa ccacagcctc
     4261 acactcaatg aggcctgttc atttctcaag atatccagac ctaccattgc cgcatcaatg
     4321 cggcttttct tgcgtgtaat tgcggagact ttgcgatgta cttgacactt caggagtgga
     4381 atgcacgcca gcgacgccca agaagccttg aaacagttcg tcgatgggtg cgcgaatgca
     4441 ggatattccc tcctccggtt aaggatggaa gagagta
//
LOCUS       DAZGJW010000259         4456 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZGJW010000259 DAZGJW010000000
VERSION     DAZGJW010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.261"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..509)
                     /locus_tag="ACHKIK_004937"
                     /pseudo
     CDS             complement(1..509)
                     /locus_tag="ACHKIK_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
     gene            complement(469..588)
                     /locus_tag="ACHKIK_004938"
                     /pseudo
     CDS             complement(469..588)
                     /locus_tag="ACHKIK_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(601..2211)
                     /locus_tag="ACHKIK_004939"
     CDS             complement(601..2211)
                     /locus_tag="ACHKIK_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFO9085928.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSNGSMTLMLKEMSNLINDEGIK"
     gene            complement(2886..3875)
                     /locus_tag="ACHKIK_004940"
     CDS             complement(2886..3875)
                     /locus_tag="ACHKIK_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085929.1"
                     /translation="MTMTKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALALAK
                     AVKTMLNGVLKSAQDNIPFPIEILPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDAKSSTGFTQGTLQQDALNICDNVVRTINSRMV
                     DVLESSKLLKTVELPVLTGSLTAKADTIMDALYENTEPSFGSEVADYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDIAYYNGEDRGVFMMAKRFTALSFGCFRHDGDHITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(3950..>4456)
                     /locus_tag="ACHKIK_004941"
     CDS             complement(3950..>4456)
                     /locus_tag="ACHKIK_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085930.1"
                     /translation="SSKASALFNNQDWKLSVTDINLYENTVSLDGQFYPLSLAIKTLI
                     PGYLSGLPSTSRESMELLEALAEAGVTIGNFFSNDLMTAYGRRQQNKRAEAERIAKEQ
                     RIQAERMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLES
                     GGNWMDNL"
BASE COUNT         1158 a         1029 c          966 g         1303 t
ORIGIN      
        1 ttacaaaatg aatggaccaa tataatgatt ttttgtctaa cactggagcg tttccgtatt
       61 ggcagactgt gactttatct aaccaaagat tcaattcccc tgttcatatt cttattagtg
      121 gttgagcagg gctcaatact ccatcaaata ctttacctac gtgcaaacga ttatatttta
      181 gttcaggatg ctttagattt aacggggaac tggataaact gagccattcc cctattaacc
      241 tttccgtttc tggcattaaa gccatcccag cgagctcaga tgaaattaag ggaaatattt
      301 ctccaagcaa tacccatcca gaccagctgc cactctcttc tgctatcggt aaccatttag
      361 cactaaattc gggcattaat aatgcaacat cttcgccatt ttctttccaa ttttgaaaag
      421 tggtttcaaa cgtatgagtg tttcgattta ctttcctcag accgaacatt aactttcatc
      481 cttctgatcg ttttcgaatt tgctttcatt ttcttgctcc tcataaggct gatgtcgttg
      541 tccttgtcgt tcatcttgct cagtcaatac ccaacgttgt gctgtaactc attatgaaaa
      601 ctatttaatt ccctcatcat tgataaggtt ggacatctct ttcaacatga gagtcattga
      661 accattcgac caatttagcg gaatgatagg gcttggcgct tcatgcaaag ttcccgactt
      721 gtggatatag ttatagcttt cgggaagtgc catttcaggt acactcctgc catttgcacc
      781 aatcatattc tcgcctgtta tttgggccaa tgatctattc ataaattgca ctgagtcatt
      841 caaatactct tcctttcttg attctttata aacaattgct gctgattttg cataccatga
      901 gtcaaaaaac cattctgctt ctgttgatgg aatgaagctc ttttctcttt tcgcatgtga
      961 attttgatcg gtatcggttt taatatcatt gaaccaaaaa tttgccgact gataattatc
     1021 tttctcgtat ctttttatac catacggacc tgctagtttt ttaactattt ttagaacctg
     1081 ctcttttctt ctggtgttta tctttgcaag atttgcagga taaataacat taagtaaagc
     1141 cgcatcggct tctctgtaat gtttatcttt ttccagataa cctggggatt ctccccctaa
     1201 atcaagctgt aacgttattc tttcataacc tttatctatg aggtggttca acctcgttgt
     1261 tgaaagaggt tcatccagtt cattggcttt agcttcgcgt agtaaatcag agacaaaaac
     1321 agagtctttc ttcgagagta agttcgacaa acgttccagt ccagaggtca ccagtgcaac
     1381 ggaagatgtg tttaatcggg catcttcttc ccatgctccg gaatcttcca taacatagaa
     1441 attggcttta tccagataag caatcagcag cgcaaccgat tttaatcggt cacctttttg
     1501 ccaatcttca gcgttaattg tatcagtgtc gattgcttgt ataaggagat caagataaag
     1561 acctaacgca tcattttgtt tatggttcca cagctgcggc ttgccttctt cctgcacatc
     1621 tgccatcacg ggggagttgc tgtcaaatcg gatatgcaca acattcattt gcccgggaac
     1681 tccatccagt cgtttggggt tgctaatgac gtcctgcatc cgcttaatct ggtcgggcgt
     1741 agacatataa tcccacaggg tgagcagaac cttttttgcg gctacactat ttccctgatc
     1801 tgatactaat gccatatagc cccataaact atccctcagc cagacagcat catagtgggt
     1861 ttctgccgct gttttgtcat cttcgaaagt agaagcaaga ataaacgttc cccagggttt
     1921 ctccgtcaac gttaactttc gtgtgttttc tgttaatgtt agcaatcctt gaatttcgtc
     1981 tttggtatag tggctcttta tttcattttg taatttggct gagtgaatca aaagacgata
     2041 attaggatac ttatctggat ttaacgataa ataactttta tctgttgctg cccccgtgga
     2101 attaagaatg ttaaatacag attcactcgt cacttctgcc cctgtcgcct ggcatgtgcc
     2161 gcacgaaaat ataccagcga gcagaagagt taatgcagat ttccctttca taaaatattc
     2221 catcaatcgt ttgttaaaag cgtagataac cacttattta acaacttgaa aataaggtgt
     2281 tttacctggg ttgttacaaa aggattgcat tgcgtaaacg ctttttattt acaacaaaat
     2341 ggggaagtat tacggcgatt gcgaaaagtc tgaagcgatt gaattaagat aatatagaaa
     2401 atcagataca gcaaaaatgg cagattgatc aggtgaagat aaattggagc gggcgaaggg
     2461 agcctacgac aaatcgaaga caaccagcta acctcttgtt tcttaaagac tgatgaatcc
     2521 agttggttgt agtgatagtc acacatttag cgatagacgg tcaagacttg aagcacgtaa
     2581 tattccctct aaaggaatag aactcacggt ctgactaccc ggcccttttg gtctgactca
     2641 gggtcgggct atggggactt ttggctcgct ctttatatta gatgaggtct cacatttttc
     2701 agtcaaaact catcgtctaa tagcccatag ccagaccacc agtcccagct ttagcaggaa
     2761 gaccacgaga tagactttgt gtaggtccat ttggtcctcc tcccttctgt attcacgaac
     2821 tagattacta gtacaaaata aaggccagac atccagttcg gaagtctgac caatagtcgt
     2881 tattgttagt tagcttcttt agtgaagctc aggcgtttga ctacaggcag gacagcagta
     2941 gccgagccag tccccatctt gatggtcgta gcagcttcag ctacaacaaa caccttgccg
     3001 agaatctcaa ggtcgtgaga ctgggagtca ccatcacgtg aaaggaccac agtaatgtgg
     3061 tccccatcgt gacggaagca accgaagctc agagccgtaa aacgcttagc catcatgaag
     3121 acgccacggt cttcaccgtt gtaataggcg atgtcagtcc ccaacatatc gacgatagcg
     3181 tcttcaccac caaagccctg cttagcagca agacgtgaca gtgccttgag gtgtgactca
     3241 tgggcgatga tgccgtaatc agcaacctca gaaccgaaag aaggctcggt gttctcgtac
     3301 agcgcgtcca tgatagtgtc tgccttagct gtcaggctac cagtcaggac cggaagctct
     3361 acagtcttca acagcttgga ggattccagt acatcgacca tgcgactatt gatggtacgg
     3421 accacgttat cgcagatgtt cagagcgtcc tgctggagcg tcccctgagt gaaaccagtg
     3481 gaagacttag cgtcccatag cttggtctgg tgggacacga ggatagcttg ctcaatcggc
     3541 tcaacggtga tttcagtacc agacggagcg gtcacgactt caggtcgctt accaccacgg
     3601 acaggaacga ccacagagcg gtcagccaga gtgtgagcaa tgattggggt cggtaagcta
     3661 ttgcgagttg gcagaatctc aatcgggaat ggaatgttat cctgagcgga cttcagcaca
     3721 ccattcagca tggtcttgac agctttagcc agtgccagag catagacctg tgggtcagca
     3781 ttggttggca gaccttcgtt aacctgaatg ttgtctgtgt cggtgatacg tactggctca
     3841 atgccgaact gatagctgtc tttagtcata gtcataataa tcaatctcct aaattagttt
     3901 agatacgtgg gtcccgacat caggtcagga ccacttgtgt gcgtctgtct tacaggttgt
     3961 ccatccagtt accgcctgac tcaaggtcag cgacgatagc ggcacgggag cgaccagcgg
     4021 aatgggtagc actacgaagg ttctcaccgt aggtccggcg ttcagctttg acctgttcac
     4081 ggcgctgtag ctctttctgc cactctgcat cagtcatgtt ctcttcacgc atgcgctcgg
     4141 cctgaatgcg ctgctcttta gcgatacgct cagcctcagc tcgcttgttc tgctggcggc
     4201 gaccataggc ggtcatcagg tcattactga agaagttacc aatggtcacg ccagcttcag
     4261 ccagggcttc caataactcc atagactcgc ggctggtaga cggcaggcct gacaggtagc
     4321 ccggaatcag ggtcttgata gcgagggaca gcggatagaa ctgaccgtcc agactcacag
     4381 tattctcgta gagattgatg tcggtgactg acagtttcca gtcttggtta ttgaataacg
     4441 cactagcttt gctaga
//
LOCUS       DAZGJW010000260         4441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZGJW010000260 DAZGJW010000000
VERSION     DAZGJW010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.262"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(180..491)
                     /locus_tag="ACHKIK_004942"
     CDS             complement(180..491)
                     /locus_tag="ACHKIK_004942"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO9085931.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            690..1388
                     /locus_tag="ACHKIK_004943"
     CDS             690..1388
                     /locus_tag="ACHKIK_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO9085932.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGRYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(1433..2332)
                     /gene="eamA"
                     /locus_tag="ACHKIK_004944"
     CDS             complement(1433..2332)
                     /gene="eamA"
                     /locus_tag="ACHKIK_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO9085933.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2527..3714
                     /gene="ydeE"
                     /locus_tag="ACHKIK_004945"
     CDS             2527..3714
                     /gene="ydeE"
                     /locus_tag="ACHKIK_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO9085934.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFTSGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPVNIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFIILALVIIAAWVLMLKGIRAKPWGQP
                     ALC"
     gene            3841..3936
                     /gene="mgtS"
                     /locus_tag="ACHKIK_004946"
     CDS             3841..3936
                     /gene="mgtS"
                     /locus_tag="ACHKIK_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO9085935.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            3936..4040
                     /gene="mgtT"
                     /locus_tag="ACHKIK_004947"
     CDS             3936..4040
                     /gene="mgtT"
                     /locus_tag="ACHKIK_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO9085936.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(4155..>4441)
                     /gene="dgcZ"
                     /locus_tag="ACHKIK_004948"
     CDS             complement(4155..>4441)
                     /gene="dgcZ"
                     /locus_tag="ACHKIK_004948"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO9085937.1"
                     /translation="YRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITHSEGHIN
                     ITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV"
BASE COUNT         1204 a         1050 c          935 g         1252 t
ORIGIN      
        1 aacttgatag caactggaac aagaaaacgt tccattccac gttcgaatga ggccattaat
       61 cccatttttt tatacctgtt gttttggata tttatacatc ccaataaagc atgtatgcca
      121 tatccatttt atgatgtgtg aaaagttagt tcaggtaata gtttaataat attagattct
      181 tactcttgca gtagtttcat cgcaaattca agaactgcct gtccgttcat ggtgccgtag
      241 tctttcatct cgataacggc tacaggcatt ttcccctgtg caacggcttc aatctctggt
      301 ttctgaaagc gaacttgcgg gccaagtaaa acaacgtcaa cttcgttatt tttaattttc
      361 cctttagcct ctgcaatcgc caatgcggaa atattaactt caagtgaaat agcgttagca
      421 tgatcaatca tacgtttaac cagcatactg gttgacatgc ccgcagcgca aactaacaat
      481 atttttttca tattttacct tactggtata taacagatta cattgtttaa atatcatatc
      541 ccgcagtagc tatacgtcct ggtacattga tcacagtaat acagtgataa agttgttcaa
      601 catcagggtg attattaaac gactcggcca tacgataaac caatcaggac tcaactcctt
      661 gttttcaatg gctcttaaaa ggtgcaaaag tgatttttca aaagattgcc cgcttactta
      721 aatccgaaat caatggcaat tcatggcatg ttggtgattt gttgccgtca gaagcggaac
      781 tcgctgttcg ttataatgtt tcgcgtaata ccctgcgtaa ggcattatcc ttgctggaag
      841 gcgaaggaat tattcacaga aagcatggtt caggaagata cattcaaaaa aagaattttg
      901 ttgcacacat tgatcacatg aacagtttca gtgaaattgc gcataaaagc ggcaaagagg
      961 cagggagtca gattatgaaa tttgaagtgc aggatgcttc acccactatt gcgactgagc
     1021 tgaatttagt gactggtgag caggtttatt acataaagag actgcgattc attgaggata
     1081 atgcagcaca attagaagaa acgtggatgt cagtagcacg ttttcctgat ttaaccgtgt
     1141 cacatatgca aaaatccaaa ttttcatata ttgagaatga atgcgggatc aaaatcattg
     1201 gcacctttga aactttctcc cctacttttc ctaccccaga aatcgccagt attttacgga
     1261 tcagcccacg ggatcccata cttaaaattc agacccaggc tgtggacagt aactctattc
     1321 cgctggatta ttcgttactt tacagcaata ttttcgagtt ccaggtaaag tactttttcc
     1381 cgcgataaaa gcagacgcac agagtgctga catcacattc tgtgcgtatg gtttagcctc
     1441 taacctttac cgctttacgc caccgcaagc caaatacatt gatatacagc ccggtcataa
     1501 tgagcaccgc acctaaaaat tgcagacccg ttaagcgttc atccaacaat agtgccgcac
     1561 ttgccagtcc gactacgggc accagtaacg ataacggtgc aacccgccag gtttcatagc
     1621 gtcccagtaa cgtcccccag atcccataac caacaattgt cgccacaaac gccagataca
     1681 tcagcgacaa gatggtggtc atatcaatag taaccagact gtgaatcatg gatgcggaac
     1741 catcgagaat cagcgaggca acaaagaagg gaatgattgg gattaaagcg ctccagatta
     1801 ccagcgacat caccgccgga cgcgttgagt gcgacatgat ctttttattg aagatgttgc
     1861 cacacgccca actaaatgct gcagccaggg tcaacataaa gccgagcatc gccacatgct
     1921 gaccgttcag actatcttcg attaacacca gtacgccaaa aatcgctaag gcgatccccg
     1981 ccaattgttt gccatgcagt cgctccccga aagtaaacgc gccaagcacg atagtaaaaa
     2041 acgcctgtgc ctgtaacacc agcgaagcca gtccagcagg cataccaaag ttaatggcgc
     2101 agaacagaaa ggcaaactgc gcaaaactga tggttaatcc ataccccaag agcaaattca
     2161 gtggtacttt cggtcgtgca acaaaaaaga tggccggaaa agcgaccagc ataaagcgca
     2221 aaccggccag catcagcggt ggcatgttat gaagccccac tttgatgacc acaaaattta
     2281 gcccccatac gaccactacc agtagcgcca acaccccatc ttttcgcgac attctaccgc
     2341 ctctgaattt catcttttgt aagcaatcaa cttagctgaa tttacttttc tttaacagtt
     2401 gattcgttag tcgccggtta cgacggcatt aatgcgcaaa taagtcgcta tacttcggat
     2461 ttttgccatg ctatttcttt acatctctaa aacaaaacat aacgaaacgc actgccggac
     2521 agacaaatga acttatccct acgacgctct accagcgccc ttcttgcctc gtcgttgtta
     2581 ttaaccatcg gacgcggcgc tacgctgcca tttatgacca tttacttgag tcgccagtac
     2641 agcctgagtg tcgatctaat cggttatgcg atgacaattg cgctcactat tggcgtcgtt
     2701 tttagcctcg gttttggtat cctggcggat aagttcgaca agaaacgcta tatgttactg
     2761 gcaattaccg ccttcaccag cggttttatt gccattcctt tagtgaataa cgtgacgctg
     2821 gtggtgctct tttttgccct tattaactgc gcctattccg tttttgctac cgtgttgaaa
     2881 gcctggtttg ccgacaatct ttcgtccacc agcaaaacga aaatcttctc aatcaactac
     2941 accatgctaa acattggctg gaccatcggt ccgccgctcg gcacgctgtt ggtgatgcag
     3001 agcatcaatc tgcccttttg gctggcagct atctgttccg cgtttcccat gcttttcatt
     3061 caaatttggg taaagcgcag cgagaaaatc atcgccacgg aaacaggcag tgtctggtcg
     3121 ccgaaagttt tattacaaga taaagcactg ttgtggttta cctgctctgg ttttctggct
     3181 tcctttgtta gcggcgcatt tgcttcatgc atttcacaat atgtgatggt gattgctgat
     3241 ggagattttg ccgaaaaggt ggtcgcggtt gttcttccgg ttaatgctgc catggtagtt
     3301 acgttgcaat attccgtggg ccgccgcctt aacccggtta acatccgcgc gctgatgaca
     3361 gcaggcaccc tctgtttcgt catcggtctg gttggtttta ttttttccgg caacagcctg
     3421 ctattgtggg gtatgtcagc tgcggtattt actgtcggtg aaatcattta tgcgccgggc
     3481 gagtatatgt tgattgacca tattgcgccg ccaggaatga aagccagcta tttttctgcc
     3541 cagtctttag gctggcttgg tgctgcaatt aacccattag tgagtggcgt agtgctaacc
     3601 agcctgccgc cttcctcgct gtttatcatc ttagcgttgg tgatcattgc tgcgtgggtg
     3661 ctgatgttaa aagggattcg tgcaaaaccg tgggggcagc ccgcgctttg ttgatttaag
     3721 tcgaacacaa taaaaattta attcagcctt cgtttaggtt acctctgcta atatctttct
     3781 cattgagatg aaaattaagg taagcgagga aacacaccac accataaacg gaggcaaata
     3841 atgctgggta atatgaatgt ttttatggcc gtactgggaa taattttatt ttctggtttt
     3901 ctggccgcgt atttcagcca caaatgggat gactaatgaa cggagataat ccctcaccta
     3961 accggcccct tgttacagtt gtgtacaagg ggcctgattt ttatgacggc gaaaaaaaac
     4021 cgccagtaaa ccggcggtga atgcttgcat ggatagattt gtgttttgct tttacgctaa
     4081 caggcatttt cctgcactga taacggatcg ttgacacagt agcatcagtt ttctcaatga
     4141 atgttaaacg gagcttaaac tcggttaatc acattttgtt cgtcaataaa catgcagcga
     4201 tttcttccgg tttgcttacc ctcatacatt gcccggtctg ctcttccaat gaccacatcc
     4261 agaggctctt caggaaatgc gcgactcaca cctgctgtca cggtaatgtt gatatgccct
     4321 tcagaatgtg tgatggcatg gttatcgact aactggcaaa ttctgacacc tgcacgacat
     4381 gcttcttcat cattagccgc tttgacaata atgataaatt cttcgccccc gtagcgataa
     4441 a
//
LOCUS       DAZGJW010000261         4349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZGJW010000261 DAZGJW010000000
VERSION     DAZGJW010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.263"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            459..719
                     /locus_tag="ACHKIK_004949"
     CDS             459..719
                     /locus_tag="ACHKIK_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9085938.1"
                     /translation="MNSISVSTPAPAAPVLPVPAQQERFIRLPEVMHLCGLSRSTIYD
                     LISRDAFPQQISLGGKNVAWVQSEVSAWMAARIAAREQGGHA"
     gene            716..1264
                     /locus_tag="ACHKIK_004950"
     CDS             716..1264
                     /locus_tag="ACHKIK_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO9085939.1"
                     /translation="MKLALQSHLLFSGLRPDIFSRYSFPAAAKSAAGRRNPSYLLATP
                     DAPCVFFYVVAQAHPFFGLRCLYLSPFQIMVVRAGQPSGWPVSNKAGIPTPVRVTTHE
                     RRNSGGGIYRYLLEVALMATILAPSHPQFIFVFAAVRRAERKPRISMLRIIASNEHHA
                     RLSLVHEYVLCLAARLPVREVI"
     gene            1264..1488
                     /locus_tag="ACHKIK_004951"
     CDS             1264..1488
                     /locus_tag="ACHKIK_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085940.1"
                     /translation="MNHAAISYDDILRLKHLRNVGEFVTGMAVLQDCYEKPASAQCEQ
                     LVSLIYLMTEQLDGVVQRCQDDLMNMEVVQ"
     gene            1485..1808
                     /locus_tag="ACHKIK_004952"
     CDS             1485..1808
                     /locus_tag="ACHKIK_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HFO9085941.1"
                     /translation="MKSRTLSLSLRVALYRRAVACAWLSACYQQNRHLQLTLDEIETA
                     IARELEGFYLRQHGQTKGMEIACALLSDLMEYGPLMSCPALSQLGIAVMDELCVRHIK
                     KPVLH"
     gene            1823..4156
                     /locus_tag="ACHKIK_004953"
     CDS             1823..4156
                     /locus_tag="ACHKIK_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO9085942.1"
                     /translation="MSGMKVSQAVKAARGHWAQILPALGVNILKNRHQPCPVCGGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVTRALNVDISEAADRIHGLTHDLSVVNSEVRTL
                     TADTDSGKDAAAALAARLLQASRESAGNTYLTHKGFPEHICHELTSAHKAGGMMFRPG
                     DLIVPLYNADGELVNIQLISGNGSKCFLKGGQVKEAYHLIEGGGSSVRRIWIAEGYAT
                     ALTIHYLTGEAVMVAFSSVNFLSLSSVAHNKYPGYQLIIAADRDLNGSGQNRAETAAK
                     ASQCDIVLPPVFGDWNDALTQYGEESTRKAILEALKPHNASPFDTMSEAEFTAMSVSE
                     KAQRVREHYRDALAVDPNGQLLSRYESGAWKVISQSDFARDVAALFQRLGAPFSSGKI
                     ASLVETLKLIVPQQQNPSRHLIGFRNGVLDTRTGLFSPHCKENWLRTLCEVDFTPPVK
                     GETLETHAPAFWRWLDRAAGHKPAKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIETLESSRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPEQIAPEERD
                     PQLKDKIARELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADPAFDFCGYL
                     EALPDTNGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPVMLKEY
                     GLHYEKRRTNQGMQTNLTLKEESNADWLPKCDEPILK"
BASE COUNT         1038 a         1019 c         1129 g         1163 t
ORIGIN      
        1 gaaaatattc ataaaaatga atttgtctga tgttcattaa acacatccgg atacatgcgg
       61 aagtaagatg gtaatttaat ttctttaatc atcggattta ttgatacata tggtgagctg
      121 tttatttttg gccgttttgg ctcttttatt aatcaggtgt tgtcttagtg cttatgatgt
      181 aacgccctga atgatatggg ctggttcaga tttgagctgt gatcctgcct agtgtaatag
      241 acttttttat tattgatgct ccttattata ttccataaag gaattgtttg tttttgttcg
      301 tttcatacgg tttttagctg ttttattttt tgagaaatca acatttcaca caaaataaat
      361 aaagcatgcg cgagccgctt atataaacat aataaggagc tacctgaatc cgcatggaat
      421 tgcccggact gttgtaaaca tattagagag gtagccagat gaatagtatt tccgtttcca
      481 ccccggcacc agctgctccg gtgctcccgg ttccggctca gcaggagcgc ttcatacgat
      541 tacctgaagt gatgcacctt tgcgggctat cccgttcaac catctacgac cttatcagcc
      601 gcgatgcctt cccgcagcag atatccctgg gtggaaaaaa tgtggcctgg gtacagtcgg
      661 aggtcagtgc atggatggcg gcgcgaattg ctgcccgcga gcaaggaggc catgcgtgaa
      721 acttgctttg cagtctcatc ttcttttttc tggcttgcgt cctgatatct tttccaggta
      781 tagttttccc gctgccgcaa aatcggctgc cgggcgtagg aacccgagtt acttattggc
      841 gacaccagac gcgccgtgcg tcttttttta cgtcgttgct caggcacacc catttttcgg
      901 gctgcggtgc ttatacctta gcccctttca gataatggtg gtccgggcgg ggcagccttc
      961 gggctggccg gtttccaata aggccggtat tcctaccccc gttcgggtca ccacccatga
     1021 gcgtaggaac tccggtggtg gcatttaccg ctacttattg gaggttgccc ttatggctac
     1081 gatcctcgcc ccgtcacatc ctcaatttat atttgttttt gccgccgttc gtcgtgcaga
     1141 acgtaaacca cgtatcagta tgctgcgtat catcgccagc aatgagcatc atgcacgtct
     1201 ttcactggtc catgagtatg tgctttgtct tgctgctcgc ctgcctgtcc gggaggtgat
     1261 ctaatgaatc acgccgcaat ctcttatgac gatattctcc gcctgaagca tcttcgtaac
     1321 gtgggagagt ttgtcactgg aatggctgtt cttcaggatt gctacgaaaa acctgccagt
     1381 gctcaatgtg aacaactggt ttccctgatt tatctgatga cagagcagct tgatggcgtg
     1441 gtacagcgtt gccaggatga cctcatgaac atggaggtgg tgcaatgaaa tcacgcactc
     1501 tttccctttc acttcgggta gcgttgtatc gccgcgcggt agcctgcgcc tggttgagtg
     1561 cctgctatca gcaaaaccgc catctgcagc tcactctgga tgaaattgaa accgctattg
     1621 cccgtgagct tgaaggattt tatctgcgac agcacgggca aacaaaaggc atggaaatcg
     1681 cctgcgccct gctttcagac ctgatggaat acggccctct aatgtcctgt cccgcactct
     1741 cccagctcgg aatagccgta atggatgagt tatgcgtccg tcacatcaaa aagccggttt
     1801 tacactgagg gagaactgcg tcatgtcagg aatgaaagtc agtcaggccg tgaaggctgc
     1861 ccgcggtcac tgggctcaga ttctgccagc gctgggcgtg aatatattga aaaatcgcca
     1921 tcagccttgc ccggtctgtg gcggtaaaga tcgttttcgc ttcgacgatc aggaaggacg
     1981 cggaacatgg ttctgcaatc agtgcggagc cggggatggt ttagcgcttg tcaccagggc
     2041 actgaatgtg gatatcagtg aagcagctga caggatacat ggactgacac atgatctgtc
     2101 tgtagttaat tccgaggtca ggacgttaac cgccgatacc gatagcggga aagatgcagc
     2161 ggcagcactt gccgcgcgac tgctgcaagc ctcgcgtgaa tctgccggaa acacttatct
     2221 tacacataaa ggtttcccgg agcatatttg ccatgagctg acttcagctc ataaggccgg
     2281 tggtatgatg ttccgccccg gtgatttgat cgtcccactc tataacgctg acggggagct
     2341 ggtgaatatt cagctcatca gtggcaatgg tagcaagtgt tttcttaaag gaggtcaggt
     2401 taaggaggcc tatcatctga tagaaggggg cgggagttct gtaagaagga tttggattgc
     2461 ggaaggttac gccacggcgc ttaccattca ttatttgaca ggagaagccg tcatggtggc
     2521 attttcgtcg gtcaactttc tttctctgag cagcgttgcc cataataagt atccgggata
     2581 tcagttaatt attgcagcgg accgcgatct gaatggttca ggccagaaca gggctgaaac
     2641 tgctgcaaaa gcgagtcagt gtgacattgt gctgccaccg gtttttggtg actggaatga
     2701 tgcgcttacc caatacggag aggaatccac ccggaaggca attcttgagg ctttgaagcc
     2761 acataacgcc agtcctttcg acacaatgag tgaagcagaa ttcacagcga tgagtgtcag
     2821 cgaaaaagct cagagggttc gggagcatta cagggatgca ctggcggttg atccgaacgg
     2881 gcagctttta tcccgctacg agtctggagc gtggaaagtt atttctcagt cagattttgc
     2941 ccgagatgtc gcagcccttt tccagcgcct cggggcaccg ttttcctcag gaaaaattgc
     3001 ctcactggtg gaaacattaa agttaattgt tccgcagcag caaaatccgt cacgtcatct
     3061 gattggtttt cgtaatggcg ttctcgatac gcgaacaggg ctttttagtc cgcactgtaa
     3121 agagaactgg ctgcgtaccc tgtgtgaagt cgatttcacg ccaccggtaa agggggaaac
     3181 gcttgaaact catgccccgg cattctggcg ttggctggac cgggccgccg gacacaagcc
     3241 agcaaaacgc gacattattc ttgcagcgtt atttatggtg ctggcgaacc gctatgactg
     3301 gcagctcttt ctggaagtga ctggccctgg tggaagtggc aaaagtattc tggctgaaat
     3361 tgcgaccatg cttgctggag aggataacgc cacctctgcg accattgaaa cgctggagtc
     3421 ctcgcgagaa cgtgcggctc tgataggttt ttctcttatt cgcctgcctg atcaggagaa
     3481 gtggagcggc gacggcgccg ggcttaaggc tataactggt ggcgatgcag tatctgtgga
     3541 ccccaaatat cagaatgctt attcaacaca tattccggcg gttatcttgg ccgtaaataa
     3601 taatcctatg cgcttcactg atcgcagtgg tggtgtgtcc cgcaggagag ttattctgca
     3661 ttttcctgag cagatagcac cggaagaacg cgatccgcag ttgaaagata aaattgcacg
     3721 agaactggct gtgattgttc gtcagctcat gcagcgtttc agcgatccga tgagcgccag
     3781 aacattgctt cagtcgcagc agaattctga tgaggctctc accatcaagc gtgatgctga
     3841 tccagcattt gatttttgtg gctaccttga ggcattacct gacaccaacg gtatgtttat
     3901 ggggaacgcc aatattgtcc cacgccagcc acgtacatac ctttaccatg cctacctcgt
     3961 atacatggag gctaatggct ataaaaacac cctgagtctt acgatgtttg gcaaagggtt
     4021 gccagtcatg ctgaaggagt acgggctaca ttatgagaaa cggcgaacta atcagggaat
     4081 gcaaactaac ctcactttga aagaggagag taatgcagac tggctgccca agtgtgatga
     4141 gccaatatta aaataacgat taactaaccc ggctgatgcc gggcttctta ttggaaacgc
     4201 acaaatgtag agatagctgt tcactcttca cctcgctgtt aacacgtaag caattgatat
     4261 ataagttaaa aatggcaggg tgaatagtgt gcatggtttt gcgaaaaaaa agtatttttt
     4321 gtagagtcaa gtcccggttt ggatccgtt
//
LOCUS       DAZGJW010000262         4343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZGJW010000262 DAZGJW010000000
VERSION     DAZGJW010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.264"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..65)
                     /locus_tag="ACHKIK_004954"
     CDS             complement(<1..65)
                     /locus_tag="ACHKIK_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscG/YscG/SsaH family type III secretion system
                     needle protein co-chaperone"
                     /protein_id="HFO9085943.1"
                     /translation="MVNDVSANKILVWAAVAAANH"
     gene            complement(71..292)
                     /gene="escF"
                     /locus_tag="ACHKIK_004955"
     CDS             complement(71..292)
                     /gene="escF"
                     /locus_tag="ACHKIK_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HFO9085944.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRSF"
     gene            complement(329..736)
                     /gene="cesD2"
                     /locus_tag="ACHKIK_004956"
     CDS             complement(329..736)
                     /gene="cesD2"
                     /locus_tag="ACHKIK_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFO9085945.1"
                     /translation="MVDTFNDEVLNHYLEQKGYTIQKEFLCGSAFFIGWRIETSFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
     gene            complement(743..1687)
                     /gene="espB"
                     /locus_tag="ACHKIK_004957"
     CDS             complement(743..1687)
                     /gene="espB"
                     /locus_tag="ACHKIK_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFO9085946.1"
                     /translation="MNTIDYTNQVMTVNSVSENTTGSNAITASAINSSLLTDGKVDVS
                     KLMLEIQKLLGKMVRILQDYQQQQLSQSYQIQLAVFESQNKAIDEKKAAATAALVGGA
                     ISSVLGILGSFAAINSATKGASDIAQKTASTSSKAIDAASDTATKTLTKATESVADAV
                     EDASSVMQQAMTTATRAASRTSDVADDIADSAQRASQLAENAADAAQKASRASRFMAA
                     VDKITGSTPFIAVTSLAEGTKTLPTTVSESVKSNHEISEQRYKSVENFQQGNLDLYKQ
                     EVRRAQDDIASRLRDMTTAARDLTDLQNRMGQSVRLAG"
     gene            complement(1708..2850)
                     /locus_tag="ACHKIK_004958"
     CDS             complement(1708..2850)
                     /locus_tag="ACHKIK_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     SctE"
                     /protein_id="HFO9085947.1"
                     /translation="MLNVNSDIQSMRSGASAATATSGINQPEVTSALDLQLVKSTAPS
                     ASWTESTALPTPPAGHSLVTPSVAEDVLSKLFGGISGEVRSRTEGTEPQRSTQNASSG
                     YPYLSQVNNVDPQAMMMMVTLLSLDASAQKVASMKNSNEIYTDGQNKALDNKTLEFKK
                     QLEEQQKAEEKAQKSKILGQVFGWLGVAVTAIAAIFNPVLWAVVAVSATAMALQTAVD
                     VMGDKAPQALKTAAQVFGGVSIAASILTAGIGGVSSLMSKVGDVANKVGSNIVKTVTK
                     LADVFVENVVSKVAATANGFTTSARSIGTTVLNNDSAQYSILAQLSAYAVQNLTRQSE
                     NLGESAKVELDKAAAELRNQASYLQNASQLISDSARVNSRIVSGRV"
     gene            complement(2863..3441)
                     /gene="espA"
                     /locus_tag="ACHKIK_004959"
     CDS             complement(2863..3441)
                     /gene="espA"
                     /locus_tag="ACHKIK_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFO9085948.1"
                     /translation="MDTSTATSVASANASTSTSTVYDLGSMSKDEVVQLFNKVGVFQA
                     ALLMFAYMYQAQSDLSIAKFADMNEASKESTTAQKMANLVDAKIADVQSSSDKNKKAK
                     LPQEVIDYINDPRNDITVSGISDLNAELGAGDLQTVKAAISAKSNNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDIQSLQYRTISAISLGK"
     gene            complement(3499..>4343)
                     /locus_tag="ACHKIK_004960"
     CDS             complement(3499..>4343)
                     /locus_tag="ACHKIK_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TyeA family type III secretion system gatekeeper
                     subunit"
                     /protein_id="HFO9085949.1"
                     /translation="ARKEEEGTTTEKLLNDMQELLTLTDSDKLKELSLKNSGLLEQHD
                     PNLAMFGNMPKGEIVALISSLLQSKFVKIELKKKYAKLLLDLLGEDDWELALLSWLGV
                     GELNQEGIQKIKKLYEKAKDEDSEKGASLLDWFMEIKDLPEREKHLKVIIRALSFDLS
                     YMSSFEDKVKTSSIISDLCRVIIFLSLDNYTDIIAISINKDKDVILNEVLSIIEHVWL
                     TEDWLLESPSRVSIVEDKHVYYFHLLKDFFASLPDACFIDREQRDNTLLMIGKVIDYK
                     EDVI"
BASE COUNT         1228 a         1017 c          798 g         1300 t
ORIGIN      
        1 ttatgatttg ccgcagctac agccgcccac accagtatct tgttggcaga aacatcatta
       61 accataaaaa ttaaaagcta cggttggaaa tggttgagac caaatctttt atcgttttca
      121 gcattccact ttctacgtta acataagcag aatattgctg aaccgcgaac tgcagctcta
      181 acattttttc agggtcatta accaaatcgg tgctattaag taactctggc acagaatcac
      241 tcagcgtttt acctacttca cccatttgtt gagtaatttc agataaattc attaaatttt
      301 cctcaatagt taatagaaat aaaaatttct aactatttac attcattacg aaccaaattc
      361 cgtcttctgt ttcttgctgc acagcgccct ttctgatgaa gtaattaaac aactcttcgc
      421 gcttttgacg ttctgaatct gaaccaatct tagatttcat cgcactgatt ttcttaatac
      481 ccgaaaaatg gtggtacaac tcttcgagca agcgagtcag tgataaaaca gggccgttaa
      541 gccctgtttg gttacgtgct tcaaaagagc acaaaatcag ttcttgttca tcaagtctgt
      601 acgctaatga gaaaaaagag gtctcaatcc gccatccgat aaaaaaggca ctgccacaaa
      661 gaaactcctt ctgtattgtg tacccttttt gttcaagata gtgattaagc acttcatcat
      721 taaacgtatc gaccatgatc aattacccag ctaagcgaac cgattgaccc atacgattct
      781 gaagatcagt gagatcgcga gcggctgttg tcatatcacg cagacggcta gcgatatcat
      841 cctgcgctct gcgaacttct tgcttataca gatccaaatt accctgctgg aagttctcca
      901 cagacttata acgctgttcg ctaatctcat ggttagattt gactgattca gataccgttg
      961 ttggcaatgt cttcgtgcct tcggcaagac tggtaacggc aataaatggt gtagagccag
     1021 taatcttatc tactgcagcc ataaagcggc ttgcccgact tgccttctga gcggcatctg
     1081 cagcgttttc agccagctga gaagctctct gagcagaatc ggcaatgtca tcagcaacgt
     1141 cggatgtacg gctggccgct ctcgttgctg tagtcatcgc ttgctgcatc acgctggatg
     1201 catcttcaac agcatcagca acgctttccg ttgccttagt caacgtttta gtcgcagtat
     1261 cagaagccgc atcaatagcc ttagaagatg tagaggcggt tttttgagca atatcactcg
     1321 cgcctttcgt agcactgtta attgctgcaa aagagcctaa gatccccaat actgatgaaa
     1381 tagccccacc aaccagagcg gctgttgcag cggccttttt ttcatcaatg gctttattct
     1441 ggctctcaaa aacggccagt tggatctgat agctctgcga caactgttgc tgttggtaat
     1501 cctgcaatat acgcaccatc ttgcccagga gtttttgaat ttccagcatc agtttagaaa
     1561 catcgacctt accatcggta agcaaagatg aattaatagc agatgcggta attgcattag
     1621 agccggtagt attctccgaa acagaattaa ccgtcattac ttgattagta taatcaatag
     1681 tattcataat taaattctct ttaataatta aactcgacca ctaacaatac ggctatttac
     1741 tcgtgctgag tcggatatta actgagaagc attttgtaaa tagcttgcct ggtttcgcaa
     1801 ctcggctgca gctttatcca gctcaacctt cgcactctca cccaggtttt cactctgtcg
     1861 agttaagttt tgtaccgcgt aagcagataa ctgagccaat atgctgtatt gcgcagaatc
     1921 attattcagc acagttgtcc caatagaacg tgcagaggtc gtaaatccat ttgccgttgc
     1981 cgcaactttt gaaacaacgt tctcaacaaa tacatcggcc agtttagtaa ctgttttcac
     2041 gatgtttgaa ccaactttgt tagcgacatc gccaacttta gacataagtg aagacacccc
     2101 gccaatgccg gctgtcagaa tacttgcagc aatagatact ccgccaaaga cttgcgctgc
     2161 tgtctttaac gcctgcgggg ccttatcgcc cattacatca actgcagttt gcagtgccat
     2221 tgctgttgcg ctaacggcaa caacagccca gagtaccggg ttaaaaatag ctgcaatagc
     2281 agttaccgcg acgcccaacc aaccaaagac ctgaccaaga attttacttt tttgtgcttt
     2341 ctcttcggct ttctgttgtt cttcgagctg ctttttaaac tccagcgttt tattatccag
     2401 cgccttattt tgcccatccg tgtagatctc gttggagttt ttcatactcg caactttctg
     2461 cgctgaagca tccagggata acagggtgac catcatcatc atggcctgag ggtcaacgtt
     2521 attcacctga gaaagataag gatagccaga gcttgcattc tgagtgctgc gctgtggttc
     2581 ggttccctct gttcgactcc taacttcacc actaatacca ccaaacagtt tactcaggac
     2641 atcctcagca acagagggcg tcactaatga gtggcctgcc ggtggcgttg gtaaagcggt
     2701 actttctgtc caacttgctg atggggccgt ggatttaacc agttgtaaat ccagcgcgga
     2761 agtcacttca ggctgattaa tacctgaagt agccgtagca gcgctggccc cagacctcat
     2821 agactggata tcgctattta cattaagcat agttatctcc ggttatttac caagggatat
     2881 tgctgaaata gttctgtatt gcagtgactg aatatcagaa cgtgcactcg ttaataggtt
     2941 taacgtattt gacatttgct gtatttcaag ctggctatta ttcactaccg tggtcaagtt
     3001 attcgatttg gccgaaatag cggccttcac cgtttgcaaa tcaccagcgc ctaattcagc
     3061 atttagatcg ctaataccac ttactgtaat gtcattgcga ggatcattta tatagtcaat
     3121 cacttcttga ggaagtttgg ctttcttatt cttgtcagaa ctactctgaa catcagcaat
     3181 tttagcatcc acaagattag ccattttttg ggctgtggtt gactccttag atgcctcatt
     3241 catatcagca aactttgcaa tcgacagatc gctttgtgcc tgatacatat aggcaaacat
     3301 gagaagcgca gcctgaaaaa caccgacttt attaaatagc tgaactactt cgtctttcga
     3361 catactgcct aagtcataga ctgtcgatgt cgaagtactc gcgttcgcac tagcaactga
     3421 tgttgcagtt gatgtatcca tgtatatacc tcttaataat ctttcttagc aagaccaaaa
     3481 acgattaaaa aaccaatatc aaataacatc ctccttataa tcaattactt taccaatcat
     3541 taataatgta ttatctctct gttccctatc aataaaacaa gcgtcaggta atgatgcaaa
     3601 aaagtctttc aataggtgga aataataaac atgtttatct tcgacaatcg atacccgaga
     3661 agggctttcc aggagccagt cttctgttaa ccatacatgt tcaataatag acaatacttc
     3721 atttaaaatg acatctttat ctttattaat agagattgca ataatatctg tatagttatc
     3781 aagcgataaa aaaatgatta cccggcataa atcgctaata attgaagatg ttttcacctt
     3841 atcttcaaaa gaagacatat aagacagatc gaacgatagc gccctaataa taacctttaa
     3901 gtgcttctca cgctctggaa gatccttaat ctccataaac cagtcaagta aagaggctcc
     3961 cttttcagaa tcctcatcct tggccttttc ataaagcttt ttgatcttct ggataccttc
     4021 ctgatttaac tcacccaccc ctaaccagga aagcagagcc aactcccaat catcttcgcc
     4081 taataaatct aataataatt tggcgtattt cttttttaat tcaatcttaa caaacttaga
     4141 ttgtaataaa gaagatataa gtgcaacaat ctcccctttt ggcatattac caaacatagc
     4201 caaattagga tcatgctgtt ccagcagccc actgttcttt aatgatagtt cttttaattt
     4261 atcgctatcg gtcagagtaa gcagctcttg catatcatta agcagcttct ccgttgtcgt
     4321 accttcctct tctttccttg cac
//
LOCUS       DAZGJW010000263         4312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZGJW010000263 DAZGJW010000000
VERSION     DAZGJW010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.265"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..132
                     /locus_tag="ACHKIK_004961"
     CDS             <1..132
                     /locus_tag="ACHKIK_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFO9085950.1"
                     /translation="KDVEESKAIERMLHFMHGILSKDEPRKYCSEACAEKDQMAHEL"
     gene            207..482
                     /locus_tag="ACHKIK_004962"
     CDS             207..482
                     /locus_tag="ACHKIK_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085951.1"
                     /translation="MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVKKMCLVHIGKNLPTEKKAEFLATLIAMKLKGEI"
     gene            584..3184
                     /gene="recE"
                     /locus_tag="ACHKIK_004963"
     CDS             584..3184
                     /gene="recE"
                     /locus_tag="ACHKIK_004963"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFO9085952.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQP
                     SGTTAVEQGEAETMEPDATEHHQDTQPLDAQSQVNSVDAKYQELRAELHEARKNIPSK
                     NPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDCVYQNQPETEKTSPDMNQPEPV
                     VQQEPEIACNACGQTGGDNCPDCGAVMGDATYQETFDEESQVEAKENDPEEMEGAEHP
                     HNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAGPGVSKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNRRTNSGKEEE
                     KAFLRECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETA
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVQPT
                     FVFLVASTTIECGRYPVEIFMMGEEAKLAGQLEYHRNLRTLADCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            3177..3986
                     /gene="recT"
                     /locus_tag="ACHKIK_004964"
     CDS             3177..3986
                     /gene="recT"
                     /locus_tag="ACHKIK_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFO9085953.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAIKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFNFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            4042..4191
                     /gene="ralR"
                     /locus_tag="ACHKIK_004965"
     CDS             4042..4191
                     /gene="ralR"
                     /locus_tag="ACHKIK_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFO9085954.1"
                     /translation="MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALER"
     gene            4157..>4312
                     /locus_tag="ACHKIK_004966"
     CDS             4157..>4312
                     /locus_tag="ACHKIK_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO9085955.1"
                     /translation="MVEVKKKHSNDEINEPLEIIMEVFMYKITATIEKEGGTPTNWTR
                     YSKSKLTK"
BASE COUNT         1336 a          987 c         1033 g          956 t
ORIGIN      
        1 aaagacgttg aagaaagcaa agctattgaa agaatgcttc acttcatgca cgggatttta
       61 tcaaaagacg aaccgagaaa atattgcagt gaagcttgtg ccgaaaaaga ccagatggca
      121 catgaacttt aattaattga ctattcgaaa ctgaatttat gccagaaatg gcaggtattc
      181 gctcaacctt aattaaggag aaaaacatga ttaccaatta tgaagccact gttgtaacta
      241 ccgatgacat tgttcacgag gtgaatctgg aaggaaagcg cattggctac gtaattaaaa
      301 cagaaaataa agaaacccca ttcactgtgg ttgatatcga tggtccatca ggcaacgtaa
      361 aaacacttga tgaaggtgtc aaaaaaatgt gcctggtgca tatcggaaag aatctgccca
      421 cagaaaaaaa agccgaattt ctggcaactc taattgcaat gaaattaaaa ggtgaaatct
      481 gaaagaaata gcctgcgtat ggcgcaggct atgaacagtg tgtatccggc aagatcattc
      541 actgaacaaa acgaatttta atctgagttg aggttaaaaa acaatgagca caaaaccact
      601 cttcctgtta cggaaagcga aaaaatcatc cggtgaacct gacgtcgtcc tgtgggcaag
      661 caacgatttt gaatcgacct gtgccactct ggactacctg atcgttaagt caggtaaaaa
      721 actgagcagc tattttaaag ctgttgccac gaattttcct gtcgttaatg acctgcccgc
      781 tgaaggtgag atcgatttta cctggagtga acgctatcaa ctcagcaaag actccatgac
      841 atgggaacta aaaccgggag cagcaccaga caacgctcac tatcaaggca ataccaacgt
      901 caacggcgaa gacatgactg agattgagga gaatatgcta ctcccaattt ctggccagga
      961 actgcccatt cgttggcttg ctcaacacgg cagcgaaaaa ccggtaacgc acgtttcacg
     1021 cgacggactc caggcattac acattgctcg ggctgaagaa ctaccggctg ttactgccct
     1081 ggctgtttcc cacaaaacca gcctgctcga cccgctggaa attcgcgaac tccacaaact
     1141 ggttcgtgac actgacaaag ttttccctaa tcctggtaat tcaaacctgg gactgataac
     1201 tgcttttttc gaagcatacc tgaacgctga ctacaccgat cgaggactgc tgacaaaaga
     1261 gtggatgaag ggtaatcgtg tttcacacat cactcgcacg gcttccggtg ctaatgctgg
     1321 cggcggaaac ctcaccgatc gcggcgaagg tttcgtacac gatctgacgt cactggcgcg
     1381 cgacgtagcc actggcgtac tggcccgttc aatggatctg gacatctata accttcatcc
     1441 ggcacacgct aaacgcattg aggaaattat cgctgaaaat aaaccgccct tttctgtttt
     1501 ccgcgacaaa ttcatcacca tgcctggcgg gctggattat tcccgcgcca tcgtggttgc
     1561 gtccgtaaaa gaagcaccaa ttgggatcga ggtcatcccc gcgcacgtca ctgaatatct
     1621 gaacaaagta ctgactgaaa ccgatcatgc caaccctgat ccggaaatcg tggatattgc
     1681 ctgcggtcgc tcctctgccc cgatgccgca gcgagtaaca gaagaaggaa aacaggatga
     1741 tgaagaaaaa ccgcaaccat ctggaacaac ggcagttgaa cagggagagg ctgaaacaat
     1801 ggaaccggac gcaactgaac atcatcagga cacgcagccg ctggatgctc agtcacaggt
     1861 aaattctgtt gatgcgaaat atcaggaact gcgggcagaa ctccatgaag cccggaaaaa
     1921 cattccatca aaaaatcctg tcgatgccga taaattgctt gctgcatcac gtggtgaatt
     1981 tgttgacgga attagcgacc cgaacgatcc gaaatgggta aaggggatcc agactcgcga
     2041 ttgtgtgtac cagaaccagc cagaaacgga aaaaaccagc ccggatatga atcaacctga
     2101 gccagtagtg caacaggaac cggaaatagc ctgcaatgcc tgcggccaga ctggcgggga
     2161 taactgccct gactgtggtg cggtgatggg cgacgcaaca taccaggaaa cattcgatga
     2221 agagagtcag gttgaagcta aggaaaatga tccggaggaa atggaaggcg ctgaacatcc
     2281 gcacaatgag aatgctggca gcgatccgca tcgcgattgc agtgatgaaa ctggcgaagt
     2341 cgcagatccc gtaatcgtag aagacataga gccaggtatt tattacggaa tttcgaatga
     2401 gaattaccac gcgggtcccg gtgtcagtaa gtctcagctc gatgacattg ctgatactcc
     2461 ggcactgtat ttgtggcgta aaaatgcccc cgtggacacc acaaagacaa aaacgctcga
     2521 tttaggaacc gctttccact gccgggtact tgagccggaa gaattcagta accgctttat
     2581 cgtagcacct gaatttaacc gccggacaaa ctccggaaaa gaagaagaga aagcgtttct
     2641 gagggaatgc gcaagcacag gaaaaacggt tatcactgcc gaagaaggcc ggaaaattga
     2701 actcatgtat cagagcgtta tggctttgcc gctggggcaa tggcttgttg aaagcgccgg
     2761 acacgctgaa tcatcaattt actgggaaga tcctgaaaca gcaattttgt gtcggtgccg
     2821 tccggacaaa attatccctg aatttcactg gatcatggac gtgaaaacta cggcggatat
     2881 tcaacgattc aaaaccgctt attacgacta ccgctatcac gttcaggatg cattctacag
     2941 tgacggttat gaagcacagt ttggagtgca gccaactttc gtttttctgg ttgccagcac
     3001 aactattgaa tgcggacgtt atccggttga aattttcatg atgggcgaag aagcaaaact
     3061 ggcaggtcag ctggaatatc accgcaatct gcgaaccctg gctgactgcc tcaataccga
     3121 tgaatggcca gctattaaga cgttatcact gccccgctgg gctaaggaat atgcaaatga
     3181 ctaagcaacc accaatcgca aaagccgatc tgcaaaaaac tcagggaaac cgtgcaccag
     3241 cagcaattaa aaataacgac gtgattagtt ttattaacca gccatcaatg aaagagcaac
     3301 tggcagcagc tcttccacgc catatgacgg ctgaacgtat gatccgtatc gccaccacag
     3361 aaattcgtaa agttccggcg ttaggaaact gtgacactat gagttttgtc agtgcaatcg
     3421 tacagtgttc acagctcgga cttgagcccg gtagcgccct cggtcatgca tatttactgc
     3481 cttttggtaa taaaaacgaa aagagcggta aaaaaaacgt tcagctaatc attggctatc
     3541 gcggcatgat tgatctggct cgccgttcag gtcaaatcgc cagcttgtca gcccgtgttg
     3601 tccgtgaagg tgacgagttt aatttcgaat ttggccttga tgaaaagtta atacaccgcc
     3661 caggagaaaa cgaagatgcc cctgttaccc acgtctatgc tgtcgcaaga ctgaaagacg
     3721 gaggtactca gtttgaagtt atgacgcgca aacagattga gctggtgcgc agccagagta
     3781 aagctggtaa taacgggccg tgggtaactc actgggaaga aatggcaaag aaaacggcta
     3841 ttcgtcgcct gttcaaatat ctgcccgtat caattgagat ccagcgtgca gtatcaatgg
     3901 atgaaaagga accactgaca atcgatcctg cagattcctc tgtattaacc ggggaataca
     3961 gtgtaatcga taattcagag gaataattca gcctggcggt gtaatgcacc gccaacttga
     4021 aatattttta tgagaaaaat tatgagatat gacaatgtta aaccatgtcc attttgtggt
     4081 tgtccatcag taacggtgaa agccatttca ggatattacc gagcgaagtg taacggatgc
     4141 gaatcccgaa ccggttatgg tggaagtgaa aaagaagcac tcgaacgatg aaataaacga
     4201 accactggaa ataataatgg aggtgttcat gtataaaatt accgccacta ttgaaaagga
     4261 aggtggcact cctactaact ggacaagata ttcaaaatct aaactaacga aa
//
LOCUS       DAZGJW010000264         4280 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZGJW010000264 DAZGJW010000000
VERSION     DAZGJW010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.266"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..149
                     /locus_tag="ACHKIK_004967"
                     /pseudo
     CDS             1..149
                     /locus_tag="ACHKIK_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005163699.1"
                     /note="catalyzes the formation of glutamate from
                     glutamine; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="ACHKIK_004968"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="ACHKIK_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO9085956.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /gene="digH"
                     /locus_tag="ACHKIK_004969"
     CDS             1828..3147
                     /gene="digH"
                     /locus_tag="ACHKIK_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO9085957.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLPQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVQELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3524..>4280
                     /gene="dosC"
                     /locus_tag="ACHKIK_004970"
     CDS             3524..>4280
                     /gene="dosC"
                     /locus_tag="ACHKIK_004970"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO9085958.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQT"
BASE COUNT         1093 a         1015 c         1016 g         1156 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg acaatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg gggccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgcta aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttatctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcacc ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaacccac gtcaatgaaa
      841 tgagtaaccc agggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggtggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc tctggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taagtcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact agttgcactg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcggctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taaccccacc agccgggccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct gcaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattta tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg cctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgcgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgca gaagtggttt cccgctatcc cgtagatggc
     2521 gtgcagtttg acgactattt ctatacggag tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcgcacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggtgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac aggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccac agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact cccattctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttacc ctcttgaact atcccactac taatcatgct tactaaagtc
     3241 aaattaacca cgcaattaac cacacttagt taaacagcca taaatcccct aataaaaatg
     3301 tatgaatatg aaaaaacata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattaa aactgtccta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgccgttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattct gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgctatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactaga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa atcctgctgg attctgattt
     4141 aggcagtagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agtggcattg ccgaagtagg ccatatctcc cgtctgattc aggatttcga
     4261 cggtatattc aatcaaacca
//
LOCUS       DAZGJW010000265         4273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZGJW010000265 DAZGJW010000000
VERSION     DAZGJW010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.267"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            381..989
                     /locus_tag="ACHKIK_004971"
     CDS             381..989
                     /locus_tag="ACHKIK_004971"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO9085959.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1087..2478
                     /gene="selA"
                     /locus_tag="ACHKIK_004972"
     CDS             1087..2478
                     /gene="selA"
                     /locus_tag="ACHKIK_004972"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO9085960.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYCQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2475..>4273
                     /gene="selB"
                     /locus_tag="ACHKIK_004973"
     CDS             2475..>4273
                     /gene="selB"
                     /locus_tag="ACHKIK_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO9085961.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVVLTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRG"
BASE COUNT          950 a         1098 c         1259 g          966 t
ORIGIN      
        1 ggattgaccg gatggccgga cgtcactccg ccggggcaca ccgaacaggc cacgccggtg
       61 ggggcaccaa tgcgcacccc ggctgaaccc tgaacaatgc taccgccata ctgcgtcatg
      121 tcgccctgac gcgccgccgg ttttccgctc atctgctcat ccttgtttta tcgggttatt
      181 tgtctgcttc caggcaaaac cctacagatt tataagaaag ccttaagtga gatatccatc
      241 agatagaaac aattaggaaa atatttatat caccatgata aatatgtgat ttatttggaa
      301 gtaacaaacc atcaacattt tctgcccttt ttcccatcac caggccatac tgatattttt
      361 cctcgcacaa aggactcggc atgaaactcg tcggtagcta caccagcccg tttgtacgca
      421 aactttctat tctgttgtta gaaaagggca taactttcga atttattaat gaactgccct
      481 ataacgcgga caacggcgtg gcgcaattta acccgttagg aaaagtgccg gtgctggtga
      541 ccgaagaggg cgaatgttgg tttgattcgc cgatcatcgc tgaatatatt gaattaatga
      601 atgtcgctcc ggcgatgttg ccgcgcgatc cgctggagtc gttgcgggtg cgcaaaattg
      661 aggcactggc ggatggcatt atggatgccg ggctggtatc ggtgcgtgaa caggcgcgtc
      721 cagcggcgca gcagtctgaa gatgaattgt tacgccagcg ggagaaaatc aaccgcagtc
      781 tggatgtgct ggaaggatat ctggtcgatg gcacactcaa aaccgatacg gtcaatctgg
      841 cgactatcgc cattgcctgt gccgtcggat atctcaattt ccgccgcgtt gcgccaggct
      901 ggtgtgtcga tcgcccacac ttagtcaaac tggtcgaaaa cctgtttagc cgcgaaagtt
      961 ttgctcgcac cgaaccgcca aaggcttgat gcgcgatatg tcctcctgac ccatctcacg
     1021 ttacaatccg tggttatgtt aaacgccctt ctccgtgtga gagggccttg atcagccagg
     1081 tttcctatga caaccgaaac gcgttccctc tatagtcaac ttccggctat tgatcgctta
     1141 ttgcgcgata gctccttcct ttctttgcgt gatacttatg gtcacacccg cgtggtggaa
     1201 ttgttgcgtc agatgctcga cgaagcgcga gaagtgattc gtggcagcca gacgctgcct
     1261 gcgtggtgtg aaaactgggc gcaagaagtc gatgcccggt tgacgaaaga agcgcagagc
     1321 gcgctgcgtc cggtgatcaa cctgacggga accgtgctgc ataccaacct tgggcgagct
     1381 ttacaggcgg aagccgcggt ggaagccgtt gcgcaggcta tgcgttcgcc agtgaccctc
     1441 gagtacgatc tggacgacgc tggacgcgga catcgcgatc gtgcgctggc gcagttgctg
     1501 tgccgcatta cgggggcgga agatgcctgt atcgttaata acaatgcggc ggcggtgtta
     1561 ttgatgttgg cggccactgc cagcgggaaa gaggtggtgg tttctcgcgg cgaactggtg
     1621 gagattggcg gcgcgtttcg tattcccgat gttatgcgtc aggcaggctg caccctacac
     1681 gaagtaggga ccaccaaccg cacgcacgcg aatgattatt gtcaggcggt gaatgaaaat
     1741 accgcactgt tgatgaaagt acataccagt aactacagca ttcaggggtt caccaaagcg
     1801 atagatgaag cggaactggt ggcgctcggc aaagagctgg atgttcccgt agtgactgat
     1861 ttaggcagtg gctcgctggt cgatcttagc cagtacggtt tgccgaaaga gccaatgccg
     1921 caggagttga ttgcggcggg cgtcagtctg gtgagtttct ccggcgacaa gttgttaggc
     1981 gggccgcagg caggaattat tgttggtaaa aaagagatga tcgcccgcct gcaaagccac
     2041 ccgctgaagc gtgcattacg cgcggataaa atgaccctcg cggcgctgga agccacgttg
     2101 cgtctttatt tacaccctga agctctgagt gaaaaattac cgaccctgcg cctgcttacc
     2161 cgcagcgcag aggtcattca aatccaggca caacgtttac aggcccccct tgccgcacat
     2221 tacggcgcgg agtttgcggt acaggttatg ccatgtcttt cgcagattgg cagtggttcg
     2281 ctgccggttg atcgcctgcc gagcgcggca ttaacgttta caccccatga tggacgcggt
     2341 agccaccttg agtcattagc cgcccgctgg cgtgaattgc cagtgccggt gattggtcgt
     2401 atttatgacg gacgattgtg gctggattta cgctgccttg aagatgagca acggtttttg
     2461 gagatgttgt tgaaatgatt attgcgactg ccggacacgt tgaccacggc aaaacaacct
     2521 tattgcaggc gattactggc gtaaatgctg accgtctgcc ggaagaaaaa aagcgcggca
     2581 tgaccatcga tctcggctat gcctactggc cgcagccgga tggtcgcgtg cctggtttta
     2641 tcgacgttcc cggtcatgaa aaatttcttt ccaacatgct ggcgggcgtt ggtggtatcg
     2701 atcacgcgct gttggtggtg gcgtgcgatg acggtgtgat ggcacagacc cgtgagcatc
     2761 tggcgatttt gcagctgacc ggtaacccga tgctgacagt ggtgctgacc aaagccgatc
     2821 gcgtggacga agcgcgtgtt gatgaggttg aacgtcaggt aaaggaggtg ctgcgggaat
     2881 acggttttgc tgaggcaaaa ctgtttatca ccgcagcaac cgaaggtcga ggaattgatg
     2941 ccctgcgcga gcatctgctt cagttaccag aacgtgaaca cgccagccaa catagtttcc
     3001 gcctcgcgat cgaccgcgca tttaccgtaa aaggtgccgg gctggtcgtc accggtacgg
     3061 cgttaagcgg ggaagtgaag gtaggcgatt cactatggct aactggtgta aataaaccga
     3121 tgcgtgtacg tgcgctgcat gcacaaaacc agccaacaga aaccgcccat gccgggcagc
     3181 gtatcgcgct taacatcgcg ggtgatgcgg aaaaagagca gattaaccgt ggcgactggc
     3241 tgcttgccga tgcgccgcca gagccgttca cacgggtgat tgtcgagctt caaacccata
     3301 cgccgctgac ccagtggcag ccgctgcata ttcaccacgc cgccagccac gttacgggac
     3361 gcgtttcgct gctggaagat aaccttgccg agctggtgtt cgacacgccg ctgtggctgg
     3421 cggataacga tcgcttagtg ctgcgcgata tctcggcccg caacacgctg gcgggggcgc
     3481 gtgtggtgat gcttaacccg ccgcgtcgcg gtaaacgtaa gccggaatat ctgcaatggc
     3541 tggcgtctct tgcacgggcg cagagcgatg ccgatgcgtt atctgttcat ctggaacgcg
     3601 gcgcggttaa ccttgcggat ttcgcctggg cgcgccagct caacggcgaa gggatgcgcg
     3661 aattgctgca acagcctggt tatattcagg ctggttatag cttgttgaat gcgccggttg
     3721 ccgcccgctg gcagcggaaa attctcgaca cattagcgac ttatcatgag caacatcgcg
     3781 atgaacctgg tcctgggcgc gaacgtctgc gacgtatggc gttgccaatg gaagatgaag
     3841 cgctggtact gttgctgatt gaaaagatgc gcgaaagcgg cgacatcctc agtcatcacg
     3901 gttggctgca tctgccggat cacaaagcgg gcttcagcga agagcagcag gccatctggc
     3961 aaaaagcaga gccgctgttt ggtgacgagc cgtggtgggt gcgtgatctg gcaaaagaga
     4021 cgggtaccga cgagcaggca atgcgcctga cgctacgcca ggcggcgcag caaggaataa
     4081 ttaccgcgat cgtcaaagat cgttattacc gtaacgatcg gattgtcgaa tttgccaata
     4141 tgatccgcga tctcgatcag gagtgtggtt caacctgcgc ggcggatttc cgcgatcgct
     4201 taggcgtagg ccgaaagctg gcaattcaga ttctggaata ttttgaccgc attggcttta
     4261 cgcgtcgtcg tgg
//
LOCUS       DAZGJW010000266         4177 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZGJW010000266 DAZGJW010000000
VERSION     DAZGJW010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.268"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..194
                     /gene="cesL"
                     /locus_tag="ACHKIK_004974"
     CDS             <1..194
                     /gene="cesL"
                     /locus_tag="ACHKIK_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesL"
                     /protein_id="HFO9085962.1"
                     /translation="ENAFFSFFNALAPERTQGVIEHIFVYDNKLCLSCLLTNIDVFSL
                     MNTFQQHVQIIERVRRMTS"
     gene            191..2218
                     /gene="escV"
                     /locus_tag="ACHKIK_004975"
     CDS             191..2218
                     /gene="escV"
                     /locus_tag="ACHKIK_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFO9085963.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSEALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMIVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLTDDTVYFEAGHAEISFEFVQEKLSANSIVYKTNEAN
                     QQLAHVSGMDVYAKTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENISIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVRGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERSEQIIGFLKNIVNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
     gene            2202..3542
                     /gene="escN"
                     /locus_tag="ACHKIK_004976"
     CDS             2202..3542
                     /gene="escN"
                     /locus_tag="ACHKIK_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFO9085964.1"
                     /translation="MILEHDSVLERYPRIQKVLNSTVPTLSLNSSTRYEGKITNIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHISGMYCGQWLSYQGEEF
                     KIRVGDELLGRLVDGIGRPMGSNITAPYLPFERSLYAEPPDPLLRQVIDQPFTLGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEFFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVKSEHLRAAAECKKLIATYKN
                     IELLIRIGEYTMGQDPEADKAIKNRKAIQSFIQQSTKDISSYEKTIESLFKVVA"
     gene            3602..3922
                     /locus_tag="ACHKIK_004977"
     CDS             3602..3922
                     /locus_tag="ACHKIK_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFO9085965.1"
                     /translation="MAKINSQIKEVDGKLDDCEQAIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQKRTKEDLQHVNKSIEKLSFAIKE
                     HYFD"
     gene            3915..>4177
                     /locus_tag="ACHKIK_004978"
     CDS             3915..>4177
                     /locus_tag="ACHKIK_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1106 domain-containing protein"
                     /protein_id="HFO9085966.1"
                     /translation="MTSISLKRNLIEPVSFQPSDDWPQGEESFNDIFNRIKKRTPDTS
                     FIRMASLWKRLFYAPGQRRRYFEQGERSFSIICGKLRGIVLTI"
BASE COUNT         1182 a          760 c          960 g         1275 t
ORIGIN      
        1 acgaaaatgc gtttttttct tttttcaatg cccttgcccc tgaacggacg cagggagtga
       61 ttgaacatat ttttgtctac gataacaaat tgtgcttaag ttgtttgtta accaatattg
      121 atgtgttttc tctaatgaat acttttcagc agcatgtgca gattattgaa cgagttcgca
      181 ggatgacatc atgaataaac tcttaaatat atttaaaaaa gcagagtcat atcacgatct
      241 tattctggct ctcttcttct ttatggctgt aatgatgatg attattccat tgccaacagt
      301 tgtggtggat atcattatcg cgattaatat atcgacagcg ttgcttttat taatgctctc
      361 aatctatata aaaaatcctc ttgagctaac ttcttttcct acaatcctgt tgattacgac
      421 gttgatgcgc ctgtcgctca gtgttagtac aaccagactt attctgctgc atcacgatgc
      481 gggtgacatt atttactcct tcggtaactt tgttgttggc ggtaatattg ttgttggcct
      541 ggttattttt accattatca ccattgtaca atttatggtt attacaaaag gggctgagcg
      601 tgttgctgaa gtgagtgctc gtttttccct tgatggtatg ccaggaaaac aaatgagtat
      661 tgatggtgat atgcgcgcag gcgttattga tccgcttgag gcaaaagttc ttcgctcccg
      721 agtgcaaaag gaaagccagt tttatggttc aatggacggg gcgatgaagt ttgtaaaagg
      781 ggacgctatt gccggtatca tcattgtttt agttaacctg tttggtggcg tgctcattgg
      841 aatgtggcaa tttgacatgc catttagtga agcacttagc ctgttttctg tattgtctgt
      901 cggtgatgct ttggttgccc agatccctgc gcttattatt tccgtcactg cgggggttgt
      961 tgttactcgt gttcccggtg aaagcgaaaa agaagaaaac cttgcaggtg atattgttca
     1021 acaggtttct gttaatagtc gccctttttt aatcagtgcc gcactcatga tcgtaatggc
     1081 gattattcct ggctttccta cattggtctt cttgtttctg gcggtttgcc tgttgggtat
     1141 cgcctggaaa ttacaaaaga aaagaacgtt tggaaccgga aataataagg acgctatggg
     1201 agctgatttg tctaatagtc aaaatatctc accgggtgcc gagccgttga ttttaaactt
     1261 aagtagtaat atttatagct cagatattac acagcagatt gaggttatgc gttggaattt
     1321 ctttgaggaa agtggaattc cattgcctaa gattattgtt aatccagtta aaaataatga
     1381 tagcgctata gaatttttgc tctatcaaga gtcaatatac aaagatactc ttacagatga
     1441 tactgtttat tttgaggctg ggcatgcaga gatatcattt gaatttgtcc aggaaaagct
     1501 ttcggctaac tctatcgtgt ataaaacgaa tgaggctaac caacaactag ctcacgtctc
     1561 aggtatggat gtttatgcaa aaacaaatga caagataacg tttttgctaa agaaacttgt
     1621 attgtctaat gccaaagagt tcatcggcgt acaagaaacg cgttatctga tggacatcat
     1681 ggagaggaaa tataacgaac ttgtgaaaga gcttcagcgt cagcttggtc tgagcaagat
     1741 tgtggatatt cttcagcgtc tcgtagagga aaatatctca attagagatc tacgaactat
     1801 ttttgaaacg cttatttttt ggtccacaaa agaaaaagat gtggttatct tgtgcgaata
     1861 cgttcgtatc gccctccgtc ggcatatttt aggtcgctat agcgttcgcg gtacacttct
     1921 gaatgtctgg cttattggct ctgatattga aaatgagtta cgagagtcga tcagacaaac
     1981 gtcatcaggt tcgtatttga atatctcacc ggagcgaagt gagcagatta ttggcttctt
     2041 aaaaaatatt gtgaatccaa cggggaatgg cgtcattctg accgctttag atatcaggcg
     2101 ctatgtgaag aaaatgattg aaggttcgtt cccgtcagtc cccgtgctct cttttcagga
     2161 agttgggaat aatatcgaac ttaaagtatt aggaacggta aatgatttta gagcatgatt
     2221 ctgtattgga aagataccca cgtatccaga aagtgctcaa tagcactgtg ccgacattat
     2281 cattaaattc gtctaccaga tatgaaggca agattaccaa tatcggcggg acgattatca
     2341 aggcgcgttt accaaaagcg cgtattggtg ccttttacaa gatagaaccc agtcaacgtt
     2401 tagctgaggt gatcgctatt gatgaagatg aggttttcct gctgcctttt gagcatattt
     2461 ccggcatgta ttgtggccag tggctaagtt atcaggggga agagttcaag attcgtgttg
     2521 gggacgaact ccttgggcga ctggttgatg gtataggcag accaatgggg agcaatatta
     2581 ctgcacctta tcttccgttt gaacgcagtt tgtatgctga accccccgat cctttattaa
     2641 ggcaggttat tgatcagcca tttacgcttg gcgtcagggc aatagatggt ttacttactt
     2701 gcggtattgg gcaacgcatc ggtattttcg cggggtccgg cgttggtaaa agtacgcttt
     2761 tgggaatgat ttgtaatggc gcatccgcgg atattattgt ccttgccctt atcggcgagc
     2821 gtggtcgcga agtaaatgaa ttcctcgcgc ttttacctca atccacgctt tctaaatgtg
     2881 tactggtcgt cacaacgtca gaccgtcccg cgctggaaag aatgaaggcc gcatttacgg
     2941 cgacaaccat tgcagagttc ttccgggatc agggcaaaaa tgtattatta atgatggatt
     3001 ccgtaactcg ttatgcccga gccgcgcgtg atgttgggct tgcatcggga gagcctgatg
     3061 taagaggggg attccctccg agtgtttttt cctcgttacc caaattatta gagcgtgcgg
     3121 gacctgcgcc aaaaggttca ataactgcga tttatacggt gctgttagaa agcgataatg
     3181 tgaatgatcc catcggtgat gaagttcgct ctattcttga tggacatatt gtgcttaccc
     3241 gagaacttgc agaggaaaac catttccctg caattgatat tggtttaagc gccagtcgtg
     3301 ttatgcataa cgttgttaaa tcggaacatt tacgtgcagc ggctgaatgc aaaaagctta
     3361 ttgcaaccta taagaatatt gaactgctta ttcgtattgg tgagtacacg atggggcaag
     3421 atcctgaagc ggataaggca ataaaaaaca ggaaagcaat tcagagcttt atacaacaaa
     3481 gcaccaaaga tatcagtagt tacgaaaaaa cgattgaaag cctattcaaa gtggttgcct
     3541 gaatatgttg gacagaattt tatctattcg taaaagcaga gcgaatcgat tgagagaatc
     3601 aatggctaag attaactctc agataaaaga ggtggatggt aaactcgatg actgcgagca
     3661 agccattaaa gagtcgattg cctctaagca ggcgtattgt gcatcgctgg tgaatctgga
     3721 taaagtgtcg ttgtataaat accagattaa aaacaatgca tttgatgagc aaaagcagcg
     3781 attatatgag aaaaaatcct cactttctaa agaaaagcgt tcattgctcg attcacaaaa
     3841 acgaacaaaa gaggatctac aacatgttaa taaatcaata gagaaattat cttttgccat
     3901 aaaggaacat tactttgact agcatttctc taaaaagaaa tttgatagag cctgtttctt
     3961 ttcagccgtc agatgattgg ccgcagggag aagaaagttt taatgatatt tttaatcgca
     4021 taaaaaaaag aacacctgac acttcgttca tacgaatggc ttctttatgg aaacgattgt
     4081 tctacgctcc tggacagagg cgacgctact ttgaacaagg agagcgttca tttagtatta
     4141 tttgtggaaa gttacgcgga attgttttaa caataaa
//
LOCUS       DAZGJW010000267         4120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZGJW010000267 DAZGJW010000000
VERSION     DAZGJW010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.269"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(94..1179)
                     /gene="dmlA"
                     /locus_tag="ACHKIK_004979"
     CDS             complement(94..1179)
                     /gene="dmlA"
                     /locus_tag="ACHKIK_004979"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO9085967.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            1282..2205
                     /gene="dmlR"
                     /locus_tag="ACHKIK_004980"
     CDS             1282..2205
                     /gene="dmlR"
                     /locus_tag="ACHKIK_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO9085968.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            2332..2970
                     /gene="leuE"
                     /locus_tag="ACHKIK_004981"
     CDS             2332..2970
                     /gene="leuE"
                     /locus_tag="ACHKIK_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO9085969.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            3143..3502
                     /gene="yeaR"
                     /locus_tag="ACHKIK_004982"
     CDS             3143..3502
                     /gene="yeaR"
                     /locus_tag="ACHKIK_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO9085970.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            3506..3694
                     /locus_tag="ACHKIK_004983"
     CDS             3506..3694
                     /locus_tag="ACHKIK_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO9085971.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(3710..>4120)
                     /gene="tnpA"
                     /locus_tag="ACHKIK_004984"
     CDS             complement(3710..>4120)
                     /gene="tnpA"
                     /locus_tag="ACHKIK_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFO9085972.1"
                     /translation="KETDIRRGRHCVFLMHVHLVFVTRYRLQIFDHDATEKLRTYFSN
                     VCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRPDIAVRYY
                     YKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPG"
BASE COUNT         1030 a          985 c         1039 g         1066 t
ORIGIN      
        1 aacaataaaa ggtgaaatga cgcaggttat ttactgcaaa gaacgaataa aaaaaccgcg
       61 tcagtgacgc ggttcaaata aactggtttg accttaacgc aaaataattt tgcaaatcgc
      121 gtcggcaacc tgtggcgtgg tggcattgcc tttcatatca ggtgttttcg gcccgtgagc
      181 aatcacttct tcaattgctg ccagaatacc gttatgcgct tgctggaaac gctcatcgcc
      241 attgccgaga aaatcgagca tcattgcacc ggcccaaatc gtggcgatag ggttagcaat
      301 gtttttcccg tagatatccg gcgcggaacc gtggactggc tcgaagagcg acgggaaagt
      361 gcgttccgga ttcaggttgg cggatggggc aatgccaatg gtgccggtgc aggccgggcc
      421 aagatcggaa aggatatcgc caaataaatt ggacgccacc accacatcga agcgttccgg
      481 ctgcatcaca aaacgcgcgc agagaatatc aatatgctgc ttgtcccagc ggatctccgg
      541 gtaattctcg gccattgctt ccactcgctc atcccagtac ggcatgctga tggctaaacc
      601 gtttgattta gtggcagaag ttagcgtctt acgtgggcgg ctttgcgcaa gttcgaaggc
      661 ataacgcaaa atgcgatcga caccacgacg cgtaaatacc gattcctgaa tgacgacttc
      721 atgctctgta ccttcattca ctctaccgcc gagcgaggaa tattcgcctt cggtgttttc
      781 cctgaccacg taaaaatcga tgtcgccagg ctgttttccc gccagcgggc agggaacgcc
      841 aggaaagaga cgaaccgggc gcaggttgac gtactggtcg aattcacgac gaaatttcag
      901 cagcgaaccc cacaacgaaa tatggtcagg aacggtgtcc ggccagccga cggcaccaaa
      961 atagatggcg tcgaaacggc taagttgctc atgccagtcg tccggcatca ttttaccgtg
     1021 atggctgtaa tactcgcagc tcgcccactc catttgctca aaactcaagg cgaagcccca
     1081 gcgctcagcg gcagcctgta acacgcgaat cccttcagga aggacttctt tgccaatccc
     1141 gtctcccggg atcgcagcaa tacgcatcgt tttcatcatt gggttctcac ttcctgacca
     1201 tcaaaattga caacatcacc atcctataat tgactgaaaa ggaattaatc cccgtaactg
     1261 gtgaaacata aaacacgaat catgaataat ttaccgctgc tgaatgattt gcgcgtcttt
     1321 atgctggtgg ctcgtcgggc tggttttgcc gccgtggcgg aagaactggg cgtttcaccg
     1381 gcgttcgtca gcaagcgcat cgccttgctg gagcaaacgc taaacgtggt gttgttacac
     1441 cgcaccaccc gccgcgtcac cattaccgaa gaaggagagc ggatttatga atgggcgcag
     1501 cggattttgc aggatgtcgg tcagatgatg gacgaacttt ctgacgtgcg tcaggtgccg
     1561 caggggatgc tgcgtattat cagcagcttt ggttttggac gacaggtggt ggctccggcg
     1621 ttatcagcgc tggctaaggc gtatccgcaa ctggaattac gttttgatgt ggaagaccgg
     1681 ttggtcgatt tggtcaatga aggtgttgat ctcgatattc gcattggcga tgacatcgcc
     1741 cctaatttaa tcgcccgcaa actggcgact aattaccgca ttctctgcgc gtcgccggaa
     1801 tttattgcgc aacatggcgc gccgaaacat ttaaccgatc tttccgcatt gccgtgcctg
     1861 gtcattaaag agcgcgatca tccttttggt gtctggcaat tacgcaataa agaaggccac
     1921 catgcgatta aagtcaccgg cccactgtcg tctaatcatg gagaaattgt ccatcagtgg
     1981 tgtctcgatg ggcaggggat tgctctgcgc tcctggtggg atgttagcga aaacattgcc
     2041 agtggtcatt tagtgcaagt gctaccggaa tattaccagc cagcgaacgt ctgggccgtt
     2101 tatgtttcaa ggctggcgac gtcagcgaaa gtgcggataa cggtagaatt tttacgccag
     2161 tattttgccg agcactaccc gaatttttca ctggagcatg cctgattaat gattcaatta
     2221 tcgggttggt atcgggttaa aacctgattt tctcctttct aagccgctac agattggtta
     2281 gcatattcac ctttaatcgc gcatgatcga aagataatta aagaggttaa tgtgttcgct
     2341 gaatacgggg ttctgaatta ctggacctat ctggttgggg ccatttttat tgtgttggtg
     2401 ccagggccaa ataccctgtt tgtactcaaa aatagcgtca gtagcggtat gaaaggcggt
     2461 tatcttgcgg cctgcggtgt atttattggc gatgcagtat tgatgtttct ggcatgggct
     2521 ggagtggcga cattaattaa gaccaccccg atattattca acattgtacg ttatcttggt
     2581 gcgttttatt tgctctatct ggggagtaaa attctctacg cgaccctgaa gggtaaaaat
     2641 aacgaggcca aatccgatga gccccaatac ggggccattt ttaaacgcgc gttaattttg
     2701 agcctgacta atccgaaagc cattttgttc tatgtgtcgt ttttcgtaca gtttatcgat
     2761 gttaatgccc cacatacggg aatttcattc tttattctgg cgacgacgct ggaactggtg
     2821 agtttctgct atttgagctt cctgattatt tctggtgctt ttgtcacgca gtacatacgt
     2881 accaaaaaga aactggctaa agttggcaac tcactgattg gtttgatgtt cgtgggtttc
     2941 gccgcccgac tggcgacgct gcaatcctga tacattcagc ccgcgttgtc gcgggctccc
     3001 cacttgtaat cctccctgat ttttctctga tatggtgcta aaaagtaacc aataaatggt
     3061 atttaaaatg caaattatca ggcgtaccct gaaacggctg gaataaaccg ttttcagcgc
     3121 attccccgaa ggagggaata ggatgctgca aatcccacag aattatattc atacgcgctc
     3181 aacgcctttc tggaataaac aaactgcgcc tgccggaata ttcgaacgtc atcttgataa
     3241 aggaacgcgc ccgggggttt acccacgcct ttccgttatg catggggcgg tcaaatatct
     3301 cggctacgct gatgaacaca gtgcagagcc tgatcaggtg atccttatcg aagcggggca
     3361 gtttgcggtg ttccctccag aaaagtggca caacattgaa gccatgactg acgatactta
     3421 tttcaacatt gacttcttcg tggctcctga agtcctgatg gaaggtgcgc aacaacggaa
     3481 agtcattcat aacgggaaat gaatcatggg caaagcaacg tataccgtga ccgtcaccaa
     3541 taacagcaat ggcgtttctg tcgattatga aacagagacg ccgatgactt tgctggtgcc
     3601 agaagtggcg gctgaagtga taaaagatct ggtgaatacc gtgcgttctt atccggtgcg
     3661 ccgtaaaacc ccgtccttca gggcggggat ataaggcgcg gttttccatc taaccaggtg
     3721 tttgctgttg ttcaatgtat tggcggatga cggatattgg cgcacctccg cagctactgg
     3781 caaaatagcc aggactccac aaaacgcctt tgtagtaata cctgactgca atatctggtc
     3841 gatctcgtcg cagtaaccta cccgatacgc ctttgaggct gtttaccaga ctggatatcg
     3901 ccagtttggg aggatagtta attaacaaat ggacgtgatc tggttcgcca tccatttcaa
     3961 caagttcagc ttcaaaatca gcacatacat ttgaaaagta agtgcgtagt ttttctgtcg
     4021 cgtcatggtc aaaaatctgg agtcggtatc tggtgacaaa gaccaggtga acatgcatca
     4081 ggaaaacaca atgccttcca cgccggatat cggtttcttt
//
LOCUS       DAZGJW010000268         4059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZGJW010000268 DAZGJW010000000
VERSION     DAZGJW010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.270"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(34..1029)
                     /locus_tag="ACHKIK_004985"
     CDS             complement(34..1029)
                     /locus_tag="ACHKIK_004985"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFO9085973.1"
                     /translation="MSYSEIVKKYENISKSTNYSIDILDKLEDDLQKIFSEKNLEFTM
                     VTTGSFGRLEASEESDIDLFIFCKKESEKHYILYKKEEIEACIKKYIHKKAGDTGTFG
                     VDAVDVFDNILKNIGGGDDTNKSLTRRMLFLLEGKAIYNKNIFNSYKKKLITKYLNSS
                     SEGKLDKYLLNDVIRYYRTITTDFQHKVDADGKSWGIRNIKLRYSRKLLYFAGVMAIA
                     HASETQQDITKRTQEISNSLDIPPLERIYNISHGKAKPELIEDIFCQYEFFLSCISDP
                     EKRASLNDIKDKSDRLNSPLYNQLSEKSTEFTDNLYKLLNNAFEKNNQIHTSLIF"
     gene            complement(1058..2086)
                     /locus_tag="ACHKIK_004986"
     CDS             complement(1058..2086)
                     /locus_tag="ACHKIK_004986"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFO9085974.1"
                     /translation="MGDFFKRNDIDEEIEKESIGYWIQDLQKSSKLLMIIKTAAFNRL
                     YGISFLGAIDYSDQNELTKKERNRAIHSLYVAGIANYVATKRNYNSDLKEHLVAAALL
                     HDIGHMPLSHSAEPFIKEKFGYGHHELGNNLISGTCWVDSGLSKALNKNFDVSFIKGL
                     LNFELKTDGHDIFSNKINADTIDGIVRCIEYKGKNKANHLNRISIAKAAFLDNIDMTE
                     NKRLQILDNFWNTKHFVYQNFINTKHSVISDKISQIFFMENKKIAYKNLLSDERRWRE
                     QYKLLFKWLSNLKSNSIPHCLKNYEMIYTTRNYKVLKTEKDIKKRYTNNKNKAKISED
                     SLLKYREE"
     gene            complement(3076..3270)
                     /locus_tag="ACHKIK_004987"
     CDS             complement(3076..3270)
                     /locus_tag="ACHKIK_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO9085975.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHEL
                     AEELQNSIQVRFESESETGT"
     gene            complement(3879..>4059)
                     /locus_tag="ACHKIK_004988"
     CDS             complement(3879..>4059)
                     /locus_tag="ACHKIK_004988"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9085976.1"
                     /translation="HLNPQHKPIYHNISLYAPQKRAERDSDAGTAKRDVLPSENRKNA
                     SGSFSAHCIAMHENE"
BASE COUNT          981 a          835 c          833 g         1410 t
ORIGIN      
        1 aaaaaaaaag gccgcttgaa agcggccttt aagttaaaaa attaatgagg tatgtatttg
       61 attgttcttt tcaaacgcat tattaagcaa tttatataaa ttatctgtaa attctgtgct
      121 tttttcgctc agttgattgt aaagagggga gtttaatcta tctgatttat cttttatgtc
      181 gtttaaacta gctcttttct ctgggtcact gatgcagctt aagaagaact catattgaca
      241 gaagatgtct tctatcagtt caggttttgc tttgccgtgg ctgatattat atattctctc
      301 taaaggtggt atatctaaac tattagatat ttcttgtgtt cgttttgtta tgtcttgttg
      361 tgtttcactg gcatgggcga tagccattac gcctgcaaaa taaagcagtt tacgggaata
      421 gcgtagtttt atatttcgga taccccaaga cttgccatcg gcatcaactt tatgctgaaa
      481 gtcagtagtg atagtcctgt aatatcttat aacatcgtta agcaaatatt tgtcgagctt
      541 tccttcgctt gaggagttca aatatttggt aattaatttt ttcttgtaac tattgaaaat
      601 gtttttgtta tatattgctt tcccttctag tagaaacaac atccttctgg ttagagattt
      661 gtttgtatca tccccaccac caatgttttt taaaatatta tcaaatacat ctaccgcatc
      721 aacgccaaac gtacctgtat caccggcttt tttatgaatg tattttttta tgcaagcctc
      781 tatttcttct tttttatata gaatgtagtg tttttccgat tcctttttac agaaaataaa
      841 gagatctata tcagattctt ctgacgcttc tagtctaccg aaagatcccg tggtgaccat
      901 ggtgaactct aaattttttt ctgaaaatat cttttgtaga tcatcctcta gtttgtcaag
      961 aatgtcaatg ctataatttg ttgattttga aatgttttcg tattttttga ctatttcact
     1021 atagctcatt ttttttatcc ctacactctt actggtatca ttcttctctg tattttaaaa
     1081 gactgtcttc acttatcttt gctttatttt tgttgtttgt atatcttttt ttaatgtctt
     1141 tctctgtctt taacacttta taatttcttg ttgtgtatat catctcgtaa ttttttaggc
     1201 aatgaggtat tgagtttgat tttaggtttg ataaccattt aaacagtaat ttatattgct
     1261 ctctccagcg tctttcgtct gagagtaagt ttttgtatgc tatcttttta ttttccatga
     1321 aaaaaatctg acttatcttg tctgatatga cgctatgttt cgtattgata aaattttgat
     1381 atacaaaatg ctttgtattc caaaaattat ctaatatctg cagcctttta ttctctgtca
     1441 tatctatgtt atctaagaat gcagcttttg caatagaaat tctatttaaa tggtttgctt
     1501 tattctttcc tttgtactct atgcacctca ctatgccatc gatagtatcg gcatttattt
     1561 tatttgagaa gatgtcgtgt ccatcggttt ttagttcgaa gtttaacagt ccttttataa
     1621 agctaacgtc aaagtttttg tttagagcct tgcttaaacc gctgtctacc cagcatgtgc
     1681 cagatattaa attatttccg agctcatgat ggccataacc aaatttttct tttataaacg
     1741 gctcagcgct atgcgataac ggcatatgtc ctatgtcatg cagtaaagct gcggctacta
     1801 aatgctcttt gagatccgaa ttgtaattcc ttttagttgc tacatagttt gctatccctg
     1861 cgacatataa agagtgaatt gctctgtttc tttctttttt tgtgagttcg ttttgatcgc
     1921 tataatcgat agcacctaag aatgatattc cgtataagcg attaaatgcg gcagttttta
     1981 ttatcattaa taatttactg gacttttgta agtcttgaat ccagtatcca atgctttctt
     2041 tttcaatctc ctcatcaatg tcatttcttt tgaagaaatc acccataata tctgctctca
     2101 aatcattcac gttaaattga ctctaacact taatatgaca acttgctcac acaatccagt
     2161 tgacgattcg tgtcatgtca agatactgtt tacctcgttg aaaatgaaaa aaccgccttg
     2221 gcgggcggtt tttcaaaggt tctctagagt tggcgctctt ttgaaccgag gtaactggct
     2281 gatagaggag cgcagtcacc aaaacttgtt ctttcagttt agccttaacc ggcgcataac
     2341 atcaagacta actcctctaa atcagttacc aatggctgct gccaatggtg cttttgcatg
     2401 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2461 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacca
     2521 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2581 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2641 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2701 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcggc gcggtctttt gctctggctt
     2761 tgcctttttg ctcacatgtt ctttccggcc ttatcccctg attctgtgga taactgtgtt
     2821 accgtttttg tgtgagtcag taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca
     2881 gtgagcgagg aagcggaaaa gcgcctgaat gtgcattttc tccttacgca tctgtgcggc
     2941 atttcacacc cggcgtgggg cacttttcat acaatctgca ctgatgccgc atggttacgc
     3001 cagtatacac tccgctatcg ctacgtgact gggtcatgtc tgcgccccga cacccgccag
     3061 ggcgcgtcag cttcgttatg ttcctgtttc actttcgctc tcaaagcgaa cctggatgct
     3121 gttctggagt tcttccgcga gttcgtgcag tcgttcgcac atggccgcct gttcgtcggc
     3181 atccagagca tccagttttt caagcagtgt caggctctgg cttttgataa atcgcgccat
     3241 attcagcgca gtttgttgct gtttgttcac cttccgttgt tctccgtcag taccgggtgt
     3301 cgggtggacc ctgaccaggt ggtaattgta agacgttgcg ggcgacggta ttacaattgc
     3361 acatcctgtc ctgtttcttt aggacgtatc atggcacagc atatcaactg ataaataatc
     3421 cctgtgcagt gcagccgctg ttgcataaaa tttcgctctg cgtcgagtgt ttcttaatca
     3481 ataattccgt tgatactccg gaaaaacgcg agtctgttct catgcctgaa atgcccacac
     3541 cccacgcaaa aacaagtttt tgctgatttt cctttataaa tagagagtta tgacaaatta
     3601 gtttctcttg ctctctttgc gatatttaaa aaagcggtgt cggcgcggtg ttgtagctgc
     3661 actgacaccg ctttgtaggg gtggtactga ctatttttat aaaaaacatc attttatatc
     3721 aggggtgctg ctggcggcgc ggtgtgtttt tttacaggac gcccctgggg gcgctgctag
     3781 gggtgtctgt tcagatatgt agcaatgagt cagggattca cgaactgaaa atgtccgaaa
     3841 atgtgtctca tgggcaccct atgcgcccaa accgttcgtc attcattttc atgcatagct
     3901 atgcagtggg ctgaaaacga tcctgacgca tttttccggt tttcactggg taaaacatct
     3961 ctttttgcag tgcctgcgtc agaatcgcgt tcagcgcgtt tctgcggtgc gtacaaactg
     4021 atgttatggt aaattggctt gtgttgagga tttaaatgc
//
LOCUS       DAZGJW010000269         4048 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZGJW010000269 DAZGJW010000000
VERSION     DAZGJW010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.271"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..281
                     /locus_tag="ACHKIK_004989"
     CDS             <1..281
                     /locus_tag="ACHKIK_004989"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO9085977.1"
                     /translation="ILTYVGEISYSLYINHMVVYSLAVLYLKPYGVLDSKGFGVFLAM
                     ICATFIISIISYNFIEKPSVSIGHAIARRMQKKSDVSSIKTESVREAP"
     gene            complement(526..1683)
                     /gene="intS"
                     /locus_tag="ACHKIK_004990"
     CDS             complement(526..1683)
                     /gene="intS"
                     /locus_tag="ACHKIK_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFO9085978.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMRGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIDANVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(1845..1919)
                     /locus_tag="ACHKIK_004991"
     tRNA            complement(1845..1919)
                     /locus_tag="ACHKIK_004991"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1884..1886),aa:Arg,seq:cct)
     gene            complement(1995..2927)
                     /gene="yfdC"
                     /locus_tag="ACHKIK_004992"
     CDS             complement(1995..2927)
                     /gene="yfdC"
                     /locus_tag="ACHKIK_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO9085979.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3221..3976
                     /gene="mlaA"
                     /locus_tag="ACHKIK_004993"
     CDS             3221..3976
                     /gene="mlaA"
                     /locus_tag="ACHKIK_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO9085980.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          993 a          960 c          948 g         1147 t
ORIGIN      
        1 aaattctaac gtatgttggt gaaatatcct attcacttta cattaatcat atggttgtat
       61 acagtcttgc tgttttatat ctcaaaccgt acggggtatt ggatagtaaa ggatttggtg
      121 tttttctagc tatgatttgc gctactttta ttatatcaat catatcttat aactttattg
      181 aaaaaccatc agtatccata gggcatgcca tcgctcgtag aatgcaaaaa aaatcagatg
      241 tatcttcaat taaaactgaa tccgttagag aagcaccttg ataaatagag caatagccag
      301 atagcaccta accgatttcg tactcatagg gttgtcactt taccaaactg agaggaaata
      361 cactgataac agttggttat gaagtactgg gtgaggtgga tgtgaatggt attttgatgc
      421 atttcattaa cctgtacgcc taagaaagca accctgaaat ctaagacaca caagctttgc
      481 actggattgc aaggctttgt gcacttcgat agtggttaag gtggttcact ccaccttttc
      541 atcaagccag tccgcccacc attgcatcat ttctctgcgc ttatcgagat actgagcatg
      601 gttgtaaata ccgcgcacag atccgccgtt ggcatgtgcc agttgcactt caatagcgtc
      661 agcaggccat tcgtgctcgt tcataatcgt gctgaattca tgcctgaatc cgtgaccgct
      721 ttccagaccc tcatagccga tttgtttgat cacaagtagc accgcgttct cgcagattgg
      781 cttcttctta tcgttgcgcc cggcaaaaac aaattctgat actggtttgg tgatggagct
      841 taacgtagtg agaagttcaa ccacctggtc cgacatcggg acaacatgaa ttttgcgtcc
      901 cttcatcaca ttggcgtcaa tggtgataat cctgttttca aaatcgacgt tcttccattg
      961 catggaacga agctcttttg ttcttagggc tgtgtagcgt aaaaccttgg tggcaatgag
     1021 cgatacgata cttcctgaaa atgttgccag tgctttattg aatgccggga tctggtctgc
     1081 aggaagaaac gggaagttct tcttgcggta tcccctcatg gcgtcagcaa ggtcaggtgc
     1141 cgggttatat ttagccctgc cggtgacaat agcgtaacgg aaaacctcgc cgcatcttct
     1201 gcgggcttta tttgctcgct ccattgcacc gcgatcttca aatctgcgga ttacttccag
     1261 cagttgcatc ggctcaatat cctgaatttc aaggccgccg atgataggca aaatgtcgtc
     1321 atcaaacatt tttgcaagtt cagtcgcata ccctactgac catacttgct tcttgtgctc
     1381 gtaccattcc ttgtaaatcg cactaaagga attgttgtta gacgaagcct ttttcgcctt
     1441 taccggatcg atgccaaccg agatgtcttt cctcgcagtc catgccttat cccttgcctc
     1501 ctgcaaagtc attagcggat attttccgac ggtcaggatt ttctccttac cgtcaatctt
     1561 gtagcgaagc tgccatacct ttttcccgga tacaggaaca taaaggtaca ggccattacc
     1621 atcgagaagg cggtatggtt tttctttcgg ctttgctgct tcaatctgct taacggtgag
     1681 catgggtaaa aatccggagg gtaaaattat tttatccact ttttacccgt catggggtgc
     1741 ggctgtcaac gatctgacgc gaaccatgac gaactgtgaa tctacggaag gcttgatatt
     1801 caggggattt tgcggactgg tacggatggg agcgaactga taaatggtgt cccctgcagg
     1861 aatcgaacct gcaattagcc cttaggaggg gctcgttata tccatttaac taagaggaca
     1921 atgcggcatg agtatacccg ctaatggact gcggggtaag tacgctgccg ctcgattgct
     1981 taaaccctcg ccatttatgc tgggttttta tcatttttct taatgttttc cgcacgttct
     2041 gctttttggc gtgcttctgc tttacgcttg ttgctcatgt cgttacgaat ctgtgcatga
     2101 ctcattaacg cgaagataaa ggtgccgccg cagatgttcc ccgctaaagt aggtagtgcg
     2161 aagggccaga tgaaatcgct ccagtgcagc gtgccgttaa acaccagata gaggatttca
     2221 acagaaccga caacgatgtg ggtggtgtca cccagtgcaa taagccaggt catcaaaata
     2281 atcaccacaa tctttgccgc acccgctgca ggaaacatcc aaaccatagt ggcgatcagc
     2341 cagccggaaa tgatcgcgtt ggcaaacatc tcgctggggg tgttcttcat cacatccatg
     2401 ccgattttga caaatgcatc gcgagtttct tcattgaaga taggcatata ttcaaatgcc
     2461 catgcagcaa tacctgtccc gagaatatta cccagcagca cgacgcccca taatcgcata
     2521 agtaagccga cgttgctcat tgtcggtttt tgcatgacgg gtagtaccgc agtcacggtg
     2581 ttttcggtaa ataattgctg gcgggccata atgacgataa taaaaccaaa ggtataaccg
     2641 agattctcca gtaagaagct gcctggcact ccttccagtt cgacatgaaa tatccctttt
     2701 gccagtagcg aagcgcccat cgacagaccc gccgcaatgg ctgaccacag tagcgccatt
     2761 gcgtcgcgtt ccagctcttt ttcaccatcc tggcggatat gctcatgaat tgccatcgcc
     2821 cgggagggga gtcggtcttc atctatttct atttttttgc cgcgctcttt ttcttcgctc
     2881 tcaacttcaa tttcgtcgct gtgttgatca attttgtcgt tgtccatggt ctcttctctt
     2941 gaattagcac gtatagctaa agcgtagcgg cttttttgct cgcaactggc gggagttact
     3001 ctgaaaatgt agaaaaggct gcgtttgcct ttttctgttt ctatagaatc aagtagccta
     3061 cagggcggcg attaccaggc tatgatcaaa tcagcaaatc agggcgtctg gacatcagtt
     3121 gacgtgctgt tacaatcgcc cacacctaaa caggcggata cggtatcgtt ccgtcatgga
     3181 tggcaaactg cataagccat aaaaaaacag ggagacattt atgaagcttc gcctgtcggc
     3241 gcttgctctg ggaactacgc ttctggtggg gtgtgcgagt tccggtacag atcagcaagg
     3301 gcgttctgac ccgttagaag ggttcaaccg caccatgtac aacttcaact tcaacgtatt
     3361 agacccgtat attgttcgac cggtcgctgt cgcctggcgt gattatgttc cgcaaccggc
     3421 gcgtaatggt ttgagcaact ttactggcaa ccttgaagaa cctgcggtga tggttaacta
     3481 cttcttgcag ggcgaccctt atcaggggat ggtccacttt acccgctttt tcctgaacac
     3541 cattttgggg atgggcggct ttattgatgt tgcagggatg gcgaacccga aactgcaacg
     3601 gactgaacct caccgcttcg gtagtacgct tggtcattat ggcgtgggtt atgggcctta
     3661 cgttcagtta ccgttctacg gtagcttcac gctgcgtgat gacggtggtg atatggcgga
     3721 tggtctttac ccggttcttt cctggctgac ctggccgatg tctgtgggta aatggacgct
     3781 tgaagggatc gaaacccgcg ctcagctgct ggattccgat ggtctgctgc gtcagtcgtc
     3841 cgatccttat attatggtgc gcgaagcgta cttccagcgt catgatttca tcgctaatgg
     3901 cggcgaactc aaaccgcagg aaaatccgaa cgcacaagcg attcaggatg atttaaaaga
     3961 tattgattct gaataagaaa caaataaaaa aggtgagtcg caaaactcac cttttttgtt
     4021 gttatccctc aattaccaca agtaaaag
//
LOCUS       DAZGJW010000270         3931 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZGJW010000270 DAZGJW010000000
VERSION     DAZGJW010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.272"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            38..1696
                     /gene="dpiB"
                     /locus_tag="ACHKIK_004994"
     CDS             38..1696
                     /gene="dpiB"
                     /locus_tag="ACHKIK_004994"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO9085981.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1665..2345
                     /gene="dpiA"
                     /locus_tag="ACHKIK_004995"
                     /gene_synonym="citB"
     CDS             1665..2345
                     /gene="dpiA"
                     /locus_tag="ACHKIK_004995"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO9085982.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2386..3771)
                     /gene="dcuC"
                     /locus_tag="ACHKIK_004996"
     CDS             complement(2386..3771)
                     /gene="dcuC"
                     /locus_tag="ACHKIK_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO9085983.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1075 a          937 c         1019 g          900 t
ORIGIN      
        1 ccgtcaaaga ggctcgggac aacccgcaag gaaaacaatg ttgcagctta acgagaataa
       61 acagtttgca tttttccaaa gactggcatt tccgctgcgt atctttttgc tgattctggt
      121 gttctcaata tttgtcattg cagccctggc gcaatatttt acggccagtt ttgaggacta
      181 tttaacgctt catgtacgcg acatggcaat gaatcaggcg aaaattattg cctccaatga
      241 cagtgtcatc tctgcggtga aaacgcgtga ctacaaacgg ctggcgacca tcgctaacaa
      301 attacaaaga gataccgatt ttgattatgt ggtgattggg gaccggcact cgatccgcct
      361 ttaccatcct aatccggaga aaattggtta tcctatgcag ttcaccaaac agggcgcgct
      421 ggagaaaggg gagagctact tcattaccgg gaaagggtca atggggatgg cgatgcgcgc
      481 caaaacgcca atctttgatg acgatggaaa agtcatcggc gtggtgtcga ttggctacct
      541 ggtgagtaaa atcgatagct ggcgggctga gtttttatta ccgatggcag gcgtgtttgt
      601 cgtgctgtta gggattctga tgttgctgtc gtggttcctg gcagcgcata tccgtcggca
      661 aatgatgggc atggagccaa agcaaatcgc acgcgtggtc cgtcagcaag aggcgctgtt
      721 tagttcggtt tatgaagggc tgattgcggt ggatccgcat ggttacatta ccgccatcaa
      781 tcgtaacgca agaaagatgc tggggctgag ctctcccgga cggcaatggt tgggtaaacc
      841 cattgctgaa gtggtcaggc ccgccgattt ctttaccgaa cagattgatg aaaaacgtca
      901 ggatgtggtg gctaacttta acggtctgag cgttattgcc aatcgggaag ctattcgttc
      961 aggtgatgat ttgctggggg ccattatcag ctttcgtagt aaagacgaaa tttccaccct
     1021 caatgcgcaa ctgacgcaaa taaaacaata cgtcgagagc cttcgtacat tgcgacacga
     1081 gcatctcaat tggatgtcga cgctcaatgg tctgttgcag atgaaagagt atgatcgcgt
     1141 gctggcgatg gtgcaggggg agtctcaggc ccagcaacag cttattgaca gcctgcgtga
     1201 ggcgtttgcc gatcgccagg tggcggggct gctttttggt aaagtgcagc gcgcccggga
     1261 actggggcta aaaatgatca ttgtccccgg tagccagctt tcgcaactgc cgccaggact
     1321 ggatagcacc gagtttgcag ccattgtggg caatttactt gataacgcct tcgaagccag
     1381 cctgcgtagc gatgaaggaa acaagatcgt tgaattattc ctcagcgatg aaggcgatga
     1441 tgtggtgatt gaagtcgccg atcagggctg cggcgttcca gagtctctac gagacaaaat
     1501 atttgagcag ggggtcagta cgcgtgctga cgagcccggt gaacatggca ttgggttgta
     1561 cttgattgcc agctacgtaa cgcgctgcgg tggtgttatc actctcgaag ataatgatcc
     1621 ctgcggtacc ttgttttcaa tctatattcc gaaagtgaaa cctaatgaca gctccattaa
     1681 ccctattgat cgttgaggac gaaacgccgc tggcagagat gcatgcggaa tatattcgtc
     1741 acattcccgg attcagtcag atattactgg cgggaaacct ggcgcaggcc cgaatgatga
     1801 tcgagcgttt taagccgggg ctaatcttgc tcgataacta tcttcctgac ggcagaggga
     1861 ttaatttatt gcatgaactg gtgcaggcgc attatcccgg cgacgtggtg tttaccactg
     1921 cagccagcga tatggaaacg gtgtctgaag cagtacgttg tggtgtattt gattatctca
     1981 ttaagcccat tgcctatgaa cggctggggc aaacgctaac ccgtttccgc cagcgtaaac
     2041 atatgctgga aagtattgat agcgccagcc agaagcaaat tgatgagatg tttaatgcct
     2101 atgctcgcgg tgaacctaaa gacgaactgc cgaccggcat tgatcccttg acgctaaacg
     2161 cggtgcgaaa actgtttaaa gagcctggtg tgcaacatac ggcagaaacg gtggcgcagg
     2221 cactgaccat cagtcgcacc actgccaggc gttatcttga atattgcgcc agccgccatc
     2281 tgattattgc tgaaattgtt cacggcaaag ttggcagacc gcaacgcata taccacagtg
     2341 ggtgacatga taaagccgga ggaaacttgc ctccggcatt actatttact tgcctgtgac
     2401 cgctgctgcc gttcctggca ccatcagctc tgtagcaacg ataacaatca ccaaaccaac
     2461 aagcaccggt accgaggtgc gttttacgac ttcaaacggc gagatcttcg ccatcccggc
     2521 aaccgcaacg actacgccag aaacgggcga aagggtacgg cccaggtttg acgcctgcag
     2581 catcgggata gtcaaatacg ccgggttaat gccggaagag tgcgccagtt tcgggatcat
     2641 ctcaacaaac gcataaaacg gcgcattgcc tgaaccggtc gtgactgccg ccagcatggt
     2701 cagaatcacc aataccagca tcaggatgat actcgccgaa ccaaacgagg tagcgataga
     2761 aatcagactt tgaataaagc cgatggtgct aagcccctga gcgaataccc cagcggcaac
     2821 cagcagcatc accacgttag caaacgcatc tgccattccg cgataagcca cttccagacc
     2881 agagaaaact ttctgggtat taaagctgcg gaggaactcc agaatggagg caatcagcat
     2941 acaaatcacc agaatagtga tgatgtgtaa ttgcggaccc catttaccgt caaaaatcag
     3001 tacaccgatg atcggcgtga acggcaaaat ggcataaaac gcaggagcag tggtggtgat
     3061 ttcactgaca tctaacattt catgagagat gtgctctttt ttatccagat aacgttgcca
     3121 gaagaagtgg gcgatcgcca tgccgataat tgcagcaatt gagataggca gcgtcgtttt
     3181 gaaggcgaag tcaatcagcg acatttcgga agcttgcgcc gccagcacca catcccctga
     3241 agtcggtgcg agaataatcg ccgccgggga ggcacaaatg gcagcagctg cgccacgact
     3301 gataccaacg tttaccatca ccggaaatag ggttgccatc agcaaaacac ccagaccggt
     3361 tgcggaagag acggccagag acatcagaca ggcgacaaaa taggcggcaa tcatcagcag
     3421 gtaaggggag ttaatatact gcaatggttt tgacgccagc ttgaccacca tatcattcgc
     3481 gccgatatgg gtcatgtaag cggcaaatcc acacagcatc ataatcatca tgccgaggtc
     3541 gccgccgcgg ctcattagta atattttaac gtattcaacg atatccgtgg cgctgtagcc
     3601 tgttgaagcc tggctggacg gtaacacttt gtgccccata atggcactga taatcagcaa
     3661 taacaggcca ccgacaaata acacaccagt ggcggaatac cctttaatga tgtagcgagc
     3721 tacacccaca ataaccacaa ccccaataag gagttcaatg aatgtcagca taatttttcc
     3781 tgtctccagg ccccaaagta aataataaaa aattcctaaa gcttaaggaa aaaatatgcc
     3841 caataaattg gcgatgaatg ctgattaaaa tcaagaaaaa ctgccattaa gacattgaag
     3901 ttgctgtttt tatacataga taacaaaacg c
//
LOCUS       DAZGJW010000271         3915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZGJW010000271 DAZGJW010000000
VERSION     DAZGJW010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.273"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            355..576
                     /locus_tag="ACHKIK_004997"
     CDS             355..576
                     /locus_tag="ACHKIK_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO9085984.1"
                     /translation="MVNANPCARQEFIWRFYSCKKHHYHFVIAATEDEARSQLPDGPC
                     IFTARFSTNSRNSLSYWNLPFSADVQEVL"
     gene            573..1610
                     /locus_tag="ACHKIK_004998"
     CDS             573..1610
                     /locus_tag="ACHKIK_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO9085985.1"
                     /translation="MKKPLVTRNDIAEAIALHTACMPTREIPGAIANYFMITRRFYTR
                     TDKAVINRLLIAEIRDYLIEQGRLRYATVAAEMRKEARRMTGNNLNVEKPAPVALATP
                     SPTVNVISNTGDTIDSQTLLKMVNEARKLCGEPEVRNNKFIEKILDELDGEHYTKSVV
                     EKMNKTSMLVITMTYKQALRVAARESKAVRRSLIDKLEELQQANSPTPSIPQTLPEAL
                     RLAAELAEQKMQLEQQLVAAAPKVDFADRVSAANGILIGNFAKVVGLKQNALFSWLRQ
                     NGILMAFGARKNVPRQQYINAGYFTVKEVVLDDENGYQIRLTPQLTGKGQQWLTRKLL
                     DAGLLKPVAIG"
     gene            1775..2575
                     /locus_tag="ACHKIK_004999"
     CDS             1775..2575
                     /locus_tag="ACHKIK_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO9085986.1"
                     /translation="MQQTFNADMNISNLHQNVDPSTTLPVICGVEITTDRAGRYNLNA
                     LHRASGLGAHKAPAQWLRTLSAKQLIEELEKETMQNCIVSFEGRGGGTFAHELLAVEY
                     AGWISPAFRLKVNQTFIDYRAGRLQPAIPQSLPEALRLAADLAEQKQRLEQKMLMDAP
                     KVEFAERVATASGVLIGNYAKVLGLGQNYLFTWLRDNGILIATGERRNVPKQEYISRG
                     YFTLKETVIDTSNGSRISFTTRITGKGQQWLMKRLLDAGVLVPVAATR"
     gene            2605..3450
                     /locus_tag="ACHKIK_005000"
     CDS             2605..3450
                     /locus_tag="ACHKIK_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO9085987.1"
                     /translation="MLAKVTFLSCITMSDFTFSGYELACFVTHSGLSRSAGHILSQCA
                     NLAATTSEYFIHKPHRLIAAETGYSQSTVVRAFREAVNKGILSVEIVIGDHRERRANL
                     YRFTPSFLAFAQQAKNALIESKLKISSAATKVKTVLAKTLALFNFLSTPPCQNDTPSP
                     CQDDVAIKNKKSQVKKTKRSVSDGAGTTSLKKLTSWIAEAKAKADNLRLSKKRAQKHE
                     FKQKVEAAARKYAYLKNKRSPDIGWISNFDNLPHCMTVNEALNAVLAKNKDNEQWGIP
                     VGFRG"
     gene            3619..>3915
                     /locus_tag="ACHKIK_005001"
     CDS             3619..>3915
                     /locus_tag="ACHKIK_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HFO9085988.1"
                     /translation="MLNRRTFNVFCDESCHLLNDHNKVMVLGALWCPGTITKKIARDI
                     KGLKLKHNLKPDFEIKWTKVSASKVEFYLEVVDYFFSNPALRFRGVVVPDKEQLD"
BASE COUNT         1149 a          927 c          877 g          962 t
ORIGIN      
        1 tatatctatt gcacgacact catctgctct aataaatttg tatttttaag tggcgaatgc
       61 tatcttttcg catcatattg accttttaat cgttcaggct tatagttccc gccgtcgtag
      121 caaattctgc gaccaggttt gacagcctga atgattgtgc ggacaaccgc agatttccga
      181 tattgcggta tttttgtgtc cgtaaaaccg cgttacgccc aaattatggt ggggcgtgat
      241 ggggaggctt cggcctgctg gtttcacaat cgccagtctg tcaaccctgt cacgtcctgc
      301 cacctgtttg acagcgggta gcaggttgtt aaacctgatt gtgaggccgt aactatggtt
      361 aatgccaatc cttgcgcacg ccaagaattc atctggcgtt tctattcctg taaaaaacac
      421 cactatcact tcgttatcgc agcaactgaa gacgaagcac gctctcaatt gcctgatggc
      481 ccctgcattt ttactgcccg tttttcaact aactcgcgca attcacttag ttactggaac
      541 ctccccttct ctgccgacgt tcaggaggtt ttatgaaaaa acctctcgtc acccgtaatg
      601 acatagccga agcgatcgcc ctgcatactg cctgtatgcc gacacgggag atccccggcg
      661 caattgccaa ctatttcatg ataaccaggc gtttttatac ccgaacagat aaggctgtga
      721 tcaacaggct actgatagcc gagatcaggg attatttgat tgaacaagga cgtctacgtt
      781 acgcaacggt ggcagcagaa atgagaaagg aggcgcgcag aatgaccggt aataatttga
      841 atgttgaaaa accagcacct gttgctttag ctacgccatc accaactgtg aatgttatct
      901 ccaacactgg agacacaatc gacagccaga cactgttaaa gatggtcaat gaagcgcgta
      961 agttatgtgg ggaaccagag gttcggaaca acaaattcat cgaaaaaata ctcgatgagc
     1021 ttgatggtga gcactacaca aaaagtgtag tggaaaaaat gaacaaaaca tcaatgcttg
     1081 tcataaccat gacctacaaa caagccctgc gagtcgccgc gcgcgaatca aaagctgtcc
     1141 gccgttcgct gatcgacaaa ctggaagaat tgcagcaggc aaactcccct accccatcga
     1201 tcccccaaac attaccagaa gctctacgcc tggctgccga gttggcagaa cagaaaatgc
     1261 agttggaaca acagctggtg gccgcagccc ctaaagtcga ttttgccgac cgggtatcag
     1321 cggctaatgg aatcctgatc gggaactttg caaaggtcgt tggacttaag caaaacgccc
     1381 ttttctcatg gttgcgccag aacggcattc tcatggcttt tggtgcgcgc aaaaacgtac
     1441 cgcgccagca gtacattaac gccgggtatt tcacggtgaa agaagtggtg ctggatgatg
     1501 aaaatggcta ccagatacgg ctgacgcccc aattaacggg taaaggccag cagtggttaa
     1561 ctcgcaagct acttgatgct ggtttgttaa aaccagtagc aataggttaa caaaagaaaa
     1621 aaacctgcca gcaaactggc aggtttctga gcagatcgtc caacccgatc tggatcgagt
     1681 cagaaaaatt tgctctaata aatttcgttt tctaagtgca aagaatcacc atttcgagct
     1741 ggtgattgaa ggttgatgca aatttggaga aaaaatgcaa caaacattca atgcggatat
     1801 gaatatatca aaccttcatc aaaatgtcga tccttcaacc actctgcccg ttatttgtgg
     1861 tgttgaaatt acgaccgacc gcgctggccg ttataacctt aatgctctac acagagcgag
     1921 cggactcggt gcccataaag cgccagctca atggctaaga acgctgtcag ccaaacagct
     1981 catcgaagag cttgaaaaag aaactatgca gaattgcata gtttcgttcg aaggccgtgg
     2041 cggcggcact tttgcccatg aattgctcgc agtggagtac gctggttgga tttctcccgc
     2101 gtttcggctg aaggtaaacc agacatttat cgactatcga gctggaagat tacaacctgc
     2161 tattccgcag agcctcccag aagctctccg tttggctgct gacctggcag agcaaaagca
     2221 acggctggag caaaaaatgc tgatggatgc acctaaagtc gaattcgccg aacgcgttgc
     2281 taccgccagc ggggttctaa tcggcaacta tgccaaagtg ctcggcctgg gccaaaacta
     2341 tctcttcacc tggttgcgtg ataacggaat tctgatcgca accggtgaac gcagaaacgt
     2401 ccccaaacaa gaatacatat cccgtgggta tttcaccctt aaagaaaccg tgatcgatac
     2461 aagcaatgga agcaggattt ctttcacgac tcgtataacc ggcaaaggtc agcagtggct
     2521 gatgaagcga ttgcttgatg ctggtgtgct ggtacctgtc gcggctacgc gctaacagac
     2581 gtagtaagaa ccaccagcat tgtaatgctg gctaaagtca ctttcctgag ctgtataacg
     2641 atgagcgatt ttactttttc tggctatgaa ttggcctgct ttgtaacaca ctccggtcta
     2701 tcccgtagcg ccgggcatat cctgtcgcaa tgtgcaaatc tcgcggcaac aaccagtgaa
     2761 tacttcattc acaagcctca ccgcctgatc gcggcagaaa ctggttatag ccaatcaacc
     2821 gtcgttcgtg cattccgtga agctgtaaac aaaggaattc tgtctgtaga gattgttatc
     2881 ggcgatcacc gtgaacgtcg cgctaacctg taccggttta caccatcctt tttggccttc
     2941 gcacaacaag ccaaaaatgc gctgattgaa agcaaattaa agatctcttc agccgcaacc
     3001 aaggttaaaa ctgttctcgc taagacattg gctttattta attttttatc cacaccccca
     3061 tgtcaaaatg ataccccctc cccctgtcag gatgacgtgg caataaagaa taagaagtca
     3121 caagttaaaa aaacaaaaag atcagtttcc gacggtgccg gaacgaccag cctcaaaaaa
     3181 ctgacttcat ggatcgctga agcaaaagca aaggctgaca atctgcggtt atccaaaaaa
     3241 cgcgctcaaa aacatgagtt caagcagaaa gtagaggcgg cagcgcggaa atatgcttac
     3301 ctgaagaaca agcgttctcc tgatattggc tggatatcaa acttcgataa tctaccgcat
     3361 tgcatgacgg taaacgaagc tcttaatgcg gtattagcca aaaataaaga taacgaacaa
     3421 tggggtatac cggtaggatt cagagggtga tagattgctc taattataac catgcatact
     3481 ttcaacacct ctagtttgcc atgaggaaaa ctcataggcg tcctggtaag atgacgtagt
     3541 tagcaaatca gaacgtccaa ttattgcaat taataaacaa ctaacggaca attctaccta
     3601 acaataagtg gagttaacat gttgaaccga agaacattta atgtattctg cgatgaatcc
     3661 tgtcacctac taaatgatca taataaagtc atggtattgg gtgcgctttg gtgtcctggc
     3721 actatcacaa aaaaaattgc tagagacatc aagggattaa aattaaaaca caatttaaaa
     3781 cctgatttcg aaatcaagtg gactaaagta tctgcgtcca aagttgaatt ttacttagag
     3841 gtcgtagatt acttcttcag caacccagca ttgcgcttta gaggtgttgt tgttcctgat
     3901 aaagagcaac tggac
//
LOCUS       DAZGJW010000272         3881 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZGJW010000272 DAZGJW010000000
VERSION     DAZGJW010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.275"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..664
                     /locus_tag="ACHKIK_005002"
     CDS             <1..664
                     /locus_tag="ACHKIK_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HFO9085989.1"
                     /translation="AIKQKITPSDIYRHALSGNILLLVYFQSPVILKKIQTFNGKIKF
                     RQFVGDLLDKLCMLDRDGFIYGQNLRLCTEARYICPVQQIIDTPLLRKLNQFRTFVRN
                     VRPGDELDVQAQVSEKNLTPPPGNSSGNLEQQIASTSQLIGSLLAEDMNSEQAANIAR
                     GWASSQTSGVMTDWLSRFGTARITLGVDEDFSLKNSRDGNPRKWRFDATNSELCAERH
                     EC"
     gene            591..2713
                     /locus_tag="ACHKIK_005003"
                     /pseudo
     CDS             591..2713
                     /locus_tag="ACHKIK_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(2883..>3881)
                     /locus_tag="ACHKIK_005004"
     CDS             complement(2883..>3881)
                     /locus_tag="ACHKIK_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO9085990.1"
                     /translation="AYNFNIEKTDFVVIHIPDQIGDAMAIFPVIRALELHKIKHLLIV
                     TSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAKNITLQYGTPDLCIEAMRK
                     KNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQNLRTPVPMTWAFMMREAG
                     FPAVRSIYELPLSDDVLDEVREEMRSLGSYIALNLDGSSQERTFSLSIAENLIAKIQS
                     ETDIPIVIVYGPKGEDKARALVDCYNNVYRLSLSPSIKRSAAIIKDAYIAITPDTSIL
                     HMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT         1138 a          883 c          877 g          983 t
ORIGIN      
        1 agccataaaa caaaaaatta ctcctagcga tatttatcgt catgccttga gtggcaacat
       61 cctactattg gtctatttcc agtctcctgt gatacttaag aagatacaaa cttttaatgg
      121 aaaaataaaa ttcaggcagt ttgtgggaga cctacttgat aaactatgca tgcttgacag
      181 ggacggtttt atttatggtc aaaatttaag actctgtaca gaagccagat atatttgtcc
      241 tgtgcaacaa attatagata cccccctgtt acgcaaactc aaccagtttc gtacgtttgt
      301 tcgtaatgtc cgcccgggtg atgaactgga tgtccaggca caagttagtg aaaaaaattt
      361 aaccccgccg ccgggtaaca gcagtggcaa ccttgagcaa cagatagcca gtacttcaca
      421 gctaattgga tctctgctcg ccgaagatat gaacagcgag caagcggcaa acatagcacg
      481 tggatgggcc tcttctcaga cttcaggggt aatgacagac tggttaagcc gcttcggtac
      541 cgcaagaatc acgctgggcg tggatgaaga tttcagcctg aagaactccc gtgacggcaa
      601 ccctcgaaaa tggcgattcg atgcaacaaa cagtgaacta tgtgccgaac gtcacgaatg
      661 ctgaaatcac gctggcagcc ttgaaggatc cggtgattgc cgacaataac gatctcacga
      721 cactaacagc accgtcgctg atacagaggg caatgcgata gccaacactg aggtaacatt
      781 tactctaccg gaagatgtga aggcgaactt cacgctgagc gatggcgaca aagcggttac
      841 tgatgccgat ggcaaagcga aagtcacgct gaaaggtaca aaagcaggcg ctcataccgt
      901 gacagcatcg atggttggcg gtaagagtga gcagttagtg gtgaacttta ctgcggatac
      961 gctcactgcg caggttaatc ttaacgttac cgaggacaat ttcatcgcca ataacattgg
     1021 aatgaccagg ctacaagcaa tagtgactga tggaaacggc aacccagtag aaggtataaa
     1081 agttaatttc cgtggaacct ccgtcacgct aagcagcacc agcgttgaaa cggatgatca
     1141 ggtttttgct gaaattcttg tgacaagcac cgaggtcgga ctaaaaacag tttcagcctc
     1201 tctggcagat aaacctactg aagtcatctc gcgattactg aatgccaagg tagatgttaa
     1261 ttctgcgacg attaccagtc aggagatacc tgaaggtcag gtcatggtcg cacaagacat
     1321 agcagttaaa gctcacgtta acgaccagtt tggcaacccg gtgacgcatc aagccgcgac
     1381 attcagtgca gcgccatcct cgcaaatgat catcagccag aacacggtct ctactaatac
     1441 gcagggagta gccgaggtca ccatgacgtc tgaaagaaac ggctcgtata ctgtaaaagc
     1501 atccctagcg aatggagctt cacttgagaa acaactggag gctattgatg aaaaactgac
     1561 actcacgtcc agtccgctta tcggtgtcaa tgctcctaaa ggcgctactc tgacggcaac
     1621 gctaacctct gcaaatggca ctccagtgga gggtcaggtc atcaacttta gcgtaacgct
     1681 agaaggtgcg acgttaagtg gcggaaaagt gagaaccaac tcttcaggtc aggctccagt
     1741 cgttctgacc agcaataaag tcggtacata tacggtgact gcatcgttcc ataacggcgt
     1801 cacaatacag acacagacaa ccgtgaaggt tactggcaac ccaagcaccg cacatgttgc
     1861 tagctttatc gctgatccgt cgactatcgc cgccaccaac agtgatttaa gtaccttaaa
     1921 ggcaacggtt gaggatggca gtggtaacct gatcgaaggt ctcactgtgt acttcgcctt
     1981 aaaaagcggc tccaccacat taacgtcatt aacagcggtg accgatcaaa acggaatcgc
     2041 gacaacaaac gtgagatgag cgataacagg gagcgtcact gtaagcgcag tcataagcgc
     2101 aggtggaatg caaacagtag atatatcgct agtggcaggc ccagcagacg cctcgcagtc
     2161 catccttaag aacaaccagt catcactgaa aggggacttt accgatagtg ctgagctaca
     2221 tcttgttctg cacgatatat caggcaatcc gatcaaagtt tctgaaggga tggaatttgt
     2281 gcaatcaggt acaaacgtgc cctatatgaa aattagcgca attgattaca gtcaaaatat
     2341 caatggtgat tacaaagcta ctgttacagg agacggagag ggtatcgcaa cgctgatccc
     2401 cgtattgaat ggtgttcatc aggcgggcct gagtaccacg atagaattca ttagcgcgga
     2461 aaccagacca atgacgggta cagtgtcagt gaatagtgca aacctaccga cagcttcatt
     2521 cccttcgcag ggatttaccg gggcgtatta tcagttgaat aatgacaact ttgcaccagg
     2581 aaaaacggcg gctgattatt cgttttcaag ctcggcctcc tgggtaggcg ttgatgctac
     2641 tggtaaagtg acattcaaaa atgacggtga tagcaataca gtaataatca cagcgccccc
     2701 aagatccggt ggtgctattt accaaacagt tcctcctgaa tcgcgttcgg tgtgactatg
     2761 atcattttcg gtgagtattg ctttgatcca aaggctggtt ttctgtgatg attgaccagc
     2821 ctcagagtat tgatgacacc accgttcata acagatcggc attactatgc ataagtactg
     2881 cctcaaattc tcgccaaaac actttttaat gcctttgcga attcatccag acttatattg
     2941 tcaatttttt gcccaacgac gactgactcc gaaacatcgg ccattgcggg ccatcgcgtt
     3001 ttgtaatcag cataaattgc aacaacaggg gtattatagg cacttgccat atgtaatatt
     3061 gaggtgtcag gagttattgc aatataagca tcttttatga ttgctgctga acgtttaatc
     3121 gagggtgata aggataaacg gtagacatta ttataacaat ccactaatgc cctggcttta
     3181 tcttcccctt tgggtccata aacgatcact attggaatat ctgtttcact ttgaattttt
     3241 gctattagat tttctgcaat cgataatgaa aatgtacgtt cctgcgagct accgtctaaa
     3301 ttgagcgcaa tgtaagatcc taacgatcgc atttcctcgc gtacctcatc gagtacatca
     3361 tcgcttaatg gcaattcata tattgacctg actgccggaa aacccgcctc acgcatcata
     3421 aatgcccatg tcatgggtac aggagtccgg agattctgat ccatccggga cgcgttctta
     3481 cacaaagggg aatagcactt catggttaaa ccgacgacct ggaaattcgt ttttgctttc
     3541 aactgactga taaatatcat cgttttcagg ttctttttac gcatcgcctc tatacaaaga
     3601 tccggcgtac cgtattgcag tgttatattc ttcgctaaat cttttatttc ttttaatgtt
     3661 gcgtgatcct gcatagtcat tgtgactaat gttaatttag tctgttcaag tttgagcgca
     3721 ttaaagactt ctaaattaat tgtcgacgta acaattaata gatgcttaat tttatgcaat
     3781 tcaagcgccc gaataacagg aaagatggcc atagcatcgc caatctgatc gggaatatgt
     3841 atgacaacaa agtctgtttt ttcaatatta aaattataag c
//
LOCUS       DAZGJW010000273         3878 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZGJW010000273 DAZGJW010000000
VERSION     DAZGJW010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.276"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..442)
                     /gene="sctJ"
                     /locus_tag="ACHKIK_005005"
     CDS             complement(<1..442)
                     /gene="sctJ"
                     /locus_tag="ACHKIK_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HFO9085991.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTALPIHIS"
     gene            complement(439..770)
                     /gene="sctI"
                     /locus_tag="ACHKIK_005006"
                     /pseudo
     CDS             complement(439..770)
                     /gene="sctI"
                     /locus_tag="ACHKIK_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            complement(790..1030)
                     /locus_tag="ACHKIK_005007"
                     /pseudo
     CDS             complement(790..1030)
                     /locus_tag="ACHKIK_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            complement(1044..2225)
                     /locus_tag="ACHKIK_005008"
     CDS             complement(1044..2225)
                     /locus_tag="ACHKIK_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO9085992.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTYPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTKQHFFSSKLFKYVMLFFLFAIIFI
                     AFYIVNASNDPQLRHIDKILVNKNRNYEVLYGRDHVIYINTNILDEAVWVKQALEKNQ
                     PGKPVRVINPDDESIRIFSWLADNFPDLQYFKLQLLDASNLRLTVSKQRNAITQQLID
                     NLIKGLLQTMPYASNISIAVLDDNVLESQAIETLSAIGLSYEKYKTANNVYFNIIGTL
                     SDSELNKINNYVDEYYKQWGKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQGSNWTFQ
                     E"
     gene            2483..2983
                     /locus_tag="ACHKIK_005009"
                     /pseudo
     CDS             2483..2983
                     /locus_tag="ACHKIK_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            2985..3278
                     /locus_tag="ACHKIK_005010"
     CDS             2985..3278
                     /locus_tag="ACHKIK_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFO9085993.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
     gene            complement(3339..3878)
                     /locus_tag="ACHKIK_005011"
                     /pseudo
     CDS             complement(3339..3878)
                     /locus_tag="ACHKIK_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
BASE COUNT         1231 a          738 c          605 g         1304 t
ORIGIN      
        1 cagaaatatg aattggtaaa gcagttttac cactatcgcc attatcaaca tcgtaactaa
       61 catgtactct cgaagaaata atgccatcca taattttgag agattgctct aaccgctgct
      121 caatagcaga atatagcctt gctttttccg ctcgtggaga tgaaacgagt gcatctgcag
      181 ggaacatctg cgatatttga atatcaggct tacccggtag attgtagatt tttagccaat
      241 ccaccgcaga agcaaaatcc gttggttcga caaatattga aaatcctgtt ttgccttgat
      301 ccttcttttc agcattaata ttatgtcttt gtaaaacagc aaggacatca ttagcttgct
      361 gttgatcaag atggttcaat aattcctgct gcttgcagcc gcacaacagc aggataaaca
      421 ataataatga tatatatttc atgagcgggt caacgtctca actgcagcga caatctttct
      481 tgctaatgtt ccaccaatga aacctcgaca ttataattaa gcacatgatc ctgcaagact
      541 aataattttt caggagttaa cccctcttta tcagacctta atgcttgtaa cgcattttgc
      601 tgccaatttt cagtttgtac agcatagttg gcaaaagcgg agatcatttt atcatccggg
      661 ttgtcaagat tgagaacccg gtcatttaaa tcaatcactt tcgcttgctg tatttcatga
      721 gcagttgatt gctcagctct atcaataggt ggcatattgc ttactaacat aataaaatcc
      781 ttatctagtt tagcggaagt tttgtatgat tgctgaatca agatctttat acgcttttac
      841 aacgttagat tgcgcatttc gatataatgt atactcagct aatgcacttt gatattcggc
      901 aagaaatttc gtgtcggagg gattggttgg ctaaatcctc caacgctttt tcaacttgct
      961 ggttcagatc atcaacgccc tgatcaaatt gattgctgat atccataata taaccattcc
     1021 aatctgccat aagtcctcct caattattcc tgaaatgtcc agttgctacc ttgagacttc
     1081 tcgaatcggt tatctccgta gctaaatgaa ctattatttg tgtcctgatt tttcaaattc
     1141 acattaaatc ttacatattg tttaccccat tgtttataat attcgtcaac atagttatta
     1201 attttattta attcactgtc acttaacgta ccaatgatat tgaaatacac attgttagct
     1261 gttttatatt tttcataaga aagacctatc gctgacaacg tttcaatagc ctgactttcc
     1321 aatacattat catctaatac cgcaatactt atattgctgg catatggcat agtttgcagt
     1381 aatcctttaa taagattatc gattagttgc tgtgtgatag cgttccgttg cttactcact
     1441 gtcaatctta gattactggc atctaatagt tgaagtttaa aatattgtaa atcagggaaa
     1501 ttatcagcaa gccatgaaaa aattcgtatc gattcatcat caggatttat cacccgtact
     1561 ggctttcctg gttgattttt ttccagtgct tgtttgaccc agactgcttc atccaaaata
     1621 ttggtattaa tatagataac atgatctcta ccatataaaa cttcataatt cctgtttttg
     1681 tttacaagaa ttttatcgat atgtctcagc tgcggatcat tactggcatt aacaatataa
     1741 aaagcaatga aaatgatagc aaaaagaaaa aaaagcatta catacttaaa cagcttactg
     1801 ctaaagaaat gttgttttgt agaaataatg ttattttcaa taatgcctgg caatgaatac
     1861 tgccaaactt catttttttc ttttacagca aatttgagac ctaaaacttc tatttgctga
     1921 ttcaatgtta acgttctatc ttcaggttct tgtgctgtta attctctgaa tgttactacc
     1981 agatcagggt aggtagtaat tattatctca aaactacctg taagctcgcc ataaggaacg
     2041 actattgtgt tttctggtaa ctcagaaaaa gcatcacttc ttcctaacga aacatcatta
     2101 ccaatgataa caagtagacg cccattaagt atttcatact cgcatccatt caatggtccg
     2161 ctcaacaaac gaatggcata agattccaat aagtcttgtg ataagaattt atcattattt
     2221 tccatgaata ccacccgcta attccataag ctctagttta gagtagtgta acaagacgga
     2281 aatttcatga ccaaattatg atgacacaga ataaaaaaat tataattccg agaactattt
     2341 taaatgcctc tatgcataac tttatttaca atttaaggta taaaccctat tccctcaatg
     2401 ttaagcaaat ataacaatta ctcttatttt aaattttaca cgtaatattt accctaaatg
     2461 attttagagt acaggtgggc atatgcaagt cttttccagt gatgtctact tcactgtagg
     2521 cactaatgca ttattagcat cccaaaaaga atattacagc gatttagttg cgttggttga
     2581 tcttggacat tcgttcgttg ttattgatga gcatcaacat cgaaatctca aaccaaatac
     2641 agaacctgta aacatccttt tgagcaacaa tttcatcagg atcaataaga atattacatt
     2701 atctgattta acgcatttcc tggtttcaaa tcttcataca caaaatgtgt attccacaca
     2761 agaaccactt acccatgatg agatagatat cctgcggtta tgtgtttctt actcgttaaa
     2821 acaaatagcc attattaaag gaattgatta taaaacaata tcatatcaca aaataagagc
     2881 cctaaataaa cttaacataa aaggtactgt ttagttgttt atagcactct gtgaatggga
     2941 taaacattat tttaaactcc aatcttgcgt aagagaaagt tgatatgtca ttagtaggat
     3001 ataatagtaa catcataata accatcgcgc tgatatcgtt gctctctgca tgcaccagag
     3061 aaaattcatt aaaggagaat caaaataaaa gaagtggcga atcccaagcg atagtagatg
     3121 caagacagtt ctttagcgaa cacccggaat atatatcatc agcagaactg gagcaaaagc
     3181 tatatcgcga atttgcacag gttactacag ttccagctta taaagagatg agtatgtatc
     3241 aattgcttat catttcacaa gatcgtttaa ctcattaaat aaggatctac acgctttttg
     3301 cgtgtagatt cttataaatt tctgactacg acagatgttt aattttttaa attatcgtct
     3361 ttattctcga tttttgtgtc ttcaccctca atgaatttat cttctgaaga gagctgttca
     3421 tcttgaacag gttgcccagc attctccaca tcttcaagcc acagcagaag acgtaatatt
     3481 tcatctatat tttcgaagct aacataatcg tagcgacgat gggtagcata attttttcgt
     3541 gctaattttt tatctgtgat aattggtatt ccgatttcct ttgcatattt ccttacgcct
     3601 aaggccacct cattagttac tcttacagaa atcaatggaa taggtgaaag atgtggctta
     3661 aagtatatcc ctattgcaat gtgagtaggg ttggaaatca tcaaacgact attactgaca
     3721 tcagacttca attgctcaga aagaatttcc tgatgcgctc tctgcgttta gacttaattt
     3781 ctggatttcc ttcttgctcc ttgtattctc ttttaacttc ttgtttatcc attttcatat
     3841 ctttcataaa taaaaaatat tcagcgataa aatcaaaa
//
LOCUS       DAZGJW010000274         3783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZGJW010000274 DAZGJW010000000
VERSION     DAZGJW010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.277"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..614
                     /locus_tag="ACHKIK_005012"
     CDS             3..614
                     /locus_tag="ACHKIK_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO9085994.1"
                     /translation="MYGTRIIYPAENKEVMVQLMNQGNRSSLLQAWIDDGDTSLPPEK
                     IQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKESIFYLNVLDIPPNSPEQEGKNA
                     LKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNGLVIKNDSANWVTISDVKANNV
                     KVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGNYISDKI"
     gene            630..3110
                     /gene="yehB"
                     /locus_tag="ACHKIK_005013"
     CDS             630..3110
                     /gene="yehB"
                     /locus_tag="ACHKIK_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO9085995.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAASYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            3124..>3783
                     /locus_tag="ACHKIK_005014"
     CDS             3124..>3783
                     /locus_tag="ACHKIK_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO9085996.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KA"
BASE COUNT         1093 a          711 c          938 g         1041 t
ORIGIN      
        1 ttatctacgg gacgcgcatt atttacccgg cagaaaataa agaagttatg gtgcagttga
       61 tgaaccaggg aaaccgttct tcgctgctgc aggcgtggat tgatgatggc gatacgtcgt
      121 taccaccaga aaaaattcag gttcctttca tgttaacgcc accagtggca aaaatagggg
      181 caaattccgg gcagcaagta aaaatcaaaa ttatgccgaa taaactgccc actaataaag
      241 aaagcatttt ttatctgaat gttctggata ttccaccaaa tagtccagag caagaaggca
      301 aaaatgcact gaagtttgcg atgcaaaaca gaattaagtt gttttaccgg ccagcgggta
      361 ttgctccggt aaataaagcg acctttaaaa agttactggt aaatcgcagt ggcaatggat
      421 tggtgataaa aaatgactca gctaattggg tgacgatttc ggatgtcaaa gctaataatg
      481 ttaaagtcaa ttatgaaact attatgattg cccccttaga aagtcagagt gttaatgtca
      541 aaagtaataa tgcaaataac tggtatctga ccattatcga tgaccacggc aactatatta
      601 gtgacaaaat ttaattgcag gagctgccta tgttgagaat gaccccactt gcatcagcaa
      661 tcgtagcgtt attgctcggc attgaagctt atgcagctga agaaaccttt gatacccatt
      721 ttatgatagg tggaatgaaa gaccagcagg ttgcaaatat tcgtcttgat gataatcaac
      781 ccttaccggg gcagtatgac atcgatattt atgtcaataa gcaatggcgc gggaaatatg
      841 agattattgt taaagacaac ccgcaagaaa catgtttatc aatagaagtt atcaagcggt
      901 taggcattaa tagcgataac ttcgccagcg gtaagcaatg tttaacattt gagcaacttg
      961 ttcagggtgg gagctatacc tgggatatcg gggtttttcg tctcgatttc agtgtcccgc
     1021 aggcctgggt ggaagaactg gaaagtggct atgttccacc ggaaaactgg gagcggggta
     1081 ttaatgcgtt ttatacctct tattatgtga gtcagtatta cagtgactat aaagcgtcgg
     1141 gtaatagtaa gagtacatat gtacgtttta acagcgggtt aaatttactg gggtggcaac
     1201 tgcattctga tgccagtttc agtaaaacaa ataacaatcc aggggtgtgg aaaagcaata
     1261 ccctgtatct ggaacgtgga tttgcccaac ttctcggcac gcttcgcgtg ggtgatatgt
     1321 acacatcaag cgatattttt gattctgttc gcttcagcgg tgtgcggttg tttcgtgata
     1381 tgcagatgtt gcctaactcg aaacaaaatt ttacgccacg ggtgcagggg attgctcaga
     1441 gtaatgcgct ggtaactatt gaacagaacg gttttgtggt ttatcagaaa gaggttcctc
     1501 ctggcccgtt cgcgattaca gatttgcagt tggccggtgg tggagcagat cttgatgtca
     1561 gcgtgaaaga ggcggacggc tcggtaacca cctatctggt gccttatgca gcagtgccaa
     1621 atatgctgca acccggcgta tcgaaatatg attttgcggc gggtcgtagc catattgaag
     1681 gggcgagcaa acaaagtgat tttgtccagg cgggttatca gtatggtttt aataatttat
     1741 tgacgctgta tggtggctcg atggtcgcga ataattatta cgcgtttact ttggggactg
     1801 gctggaatac acgcattggt gccatttccg tcgatgccac gaagtcgcat agtaaacaag
     1861 acaacggcga tgtgtttgac gggcaaagtt atcaaattgc ctacaacaaa tttgtgagcc
     1921 aaacgtcgac gcgttttggt ctggcggcct ggcgttattc atcgcgtgat taccggacat
     1981 ttaatgatca cgtttgggca aacaataaag ataattatcg ccgtgatgaa aacgatgtct
     2041 atgacattgc cgattattac cagaacgatt ttggccgtaa aaatagcttc tctgccaata
     2101 tgagccagtc attgccagaa ggctgggggt ctgtgtcctt aagtacgtta tggcgagatt
     2161 actgggggcg tagcggcagc agtaaggatt atcagttgag ttattccaat aactggcggc
     2221 ggataagcta tatcctcgcg gcaagccagg cgtatgacga gaatcatcat gaagagaaac
     2281 gttttaatat ttttatatcg attccctttg attggggtga tgacgttacg acgcctcgtc
     2341 ggcaaatata tatgtctaac tcaacgacgt ttgatgatca ggggtttgcc tcaaataata
     2401 cgggattatc aggaaccgtt ggaagccggg atcagtttaa ctatggggtc aacctgagtt
     2461 atcagaatca gggaaatgaa acgacagctg gggcgaattt gacctggaac gcgccggttg
     2521 cgacagtgaa tggcagttat agtcagtcga gtacttatcg acaggctgga gccagtgttt
     2581 cagggggcat tgtcgcctgg tcggggggcg ttaatctggc gaaccgtctt tccgaaacgt
     2641 ttgctgtgat gaatgcgcca ggaattaaag atgcttatgt caatgggcaa aaatatcgca
     2701 caacaaaccg taatggagtg gtggtatacg acggaatgac accttatcgg gaaaatcacc
     2761 tgatgctgga tgtgtcgcaa agcgatagcg aagcagaatt acgtggcaac cggaaaattg
     2821 ccgcctctta tcgcggcgcg gttgtactgg ttaattttga taccgatcag cgcaagccct
     2881 ggtttataaa agcgttaaga gcggatgggc aaccattaat gtttggttat gaagtcaatg
     2941 atatccatgg tcataatatt ggcgttgtcg gtcagggaag ccagttattt attcgcacca
     3001 atgaagtacc gccatcggtt aatgtagcaa ttgataagca acaaggactt tcatgcacaa
     3061 tcaccttcgg taaagagatt gatgaaagta gaaactatat ttgccagtaa tagtcaggtg
     3121 gttatgaata ttcgtataat gttatttata gtaatgatga tggttatgcc tgtgagctat
     3181 gcggcatgtt atagtgagtt atctgttcag cacaatttgg ttgttcaggg ggattttgca
     3241 cttactcaaa cacaaatggc gacatatgag cataatttta atgattcgtc atgcgtgagt
     3301 acaaatacta tcacccctat gagcccgtcg gatattattg ttggacttta taacgatacc
     3361 attaaattaa atttacattt tgaatggacc aataaaaaca acatcacgtt atcaaataat
     3421 cagaccagtt tcaccagtgg ttattcggta acggtgacac ctgcggccag taatgcaaaa
     3481 gtgaatgttt ctgcgggggg cggcggttca gtgatgatta atggagttgc gacattatcc
     3541 agtgcttcat catcgacacg cgggagtgcc gcagtacaat ttctactgtg tttattaggt
     3601 ggcaagtcat gggatgcatg tgtaaatagc tacagaaatg cattggcaca aaatgcaggt
     3661 gtctattcct ttaatctgac attgtcatac aacccgataa ccacaacctg caaaccggac
     3721 gatttattaa ttactttaga cagtattccc gtttcacaat taccagccac aggtaacaaa
     3781 gca
//
LOCUS       DAZGJW010000275         3749 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZGJW010000275 DAZGJW010000000
VERSION     DAZGJW010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.278"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(31..438)
                     /locus_tag="ACHKIK_005015"
                     /pseudo
     CDS             complement(31..438)
                     /locus_tag="ACHKIK_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(672..1349)
                     /locus_tag="ACHKIK_005016"
     CDS             complement(672..1349)
                     /locus_tag="ACHKIK_005016"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO9085997.1"
                     /translation="MWHYLHYKVSRGKPHAECDAKRRAGSPPGPDAHQLQRSQHKKSR
                     QQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADARRIVSHYEQRWLIEEYHKAWK
                     SGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGSISEETQNDSCEKILTPTEWKLLWV
                     KLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPGWVVMWDGWFRHQDMAEGYLVM
                     KSLDQEI"
     gene            1547..3166
                     /locus_tag="ACHKIK_005017"
     CDS             1547..3166
                     /locus_tag="ACHKIK_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEc38 family transposase"
                     /protein_id="HFO9085998.1"
                     /translation="MGNSMHSLKTLLQLPCGWRCSRQIISSDGITLHLHGKRKKAQCP
                     ECFKRSDSVHSCRRRRIQHLPCSGHTLWLVFSVRHWYCRNPACSRKIFAGSLAPFAGS
                     HQQSSQALQNLQRQLGLIAGGEAGKRAATAVGLRCSADTLLRRVINTPETKQSGAPHV
                     GIDEWAWHRGHRYGTLIVNLDTHRPLVLLPGRDQRTLATWFRKYPEIQIVSRDRSGVY
                     ATAAREGAPQARQVADRWHLLKNIGDALERMMYRHIPLIRLVASELSPKKSPDPEPSV
                     PAASLRRPERLKQQTRKKRHQRWTEVMALHNKECSFREISRITGLSRVTVSRWVRSGT
                     FPEMSTRPPKRGLLDPWREWLKEQRESSNYNASRIWREMVARGFTGSETIVRDAVAKW
                     RKGWNPPVTTAVRLPSVSRVSRWLMPWRITRDEENYASRFISLMCEKEPELKIAQQLA
                     LEFYRILKTQNKSQLSSWFNRVHESGSAEFRRVAAGMEADAAAICEAISSRWSNGVVE
                     GHVNRLKMLKRQMYGRAGFELLRQRVMSPLA"
     gene            3163..>3749
                     /locus_tag="ACHKIK_005018"
     CDS             3163..>3749
                     /locus_tag="ACHKIK_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HFO9085999.1"
                     /translation="MKISTQVAEEPLSVVDTDDIFLLKQRLAEQEALIHALQEKLSNR
                     EREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLHKESDTLTGRVYDPAVQR
                     PLRQTRTRKPFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTTEQLELMRSAFRVIR
                     TVREKHACTQCDAIVQAPAPSRPIERGIARPGLLAR"
BASE COUNT          901 a          984 c          943 g          921 t
ORIGIN      
        1 agccagtttc agattgaaca tattcagcta ttcaaagagc aattcttcat taaactcaat
       61 aaattgttcc ctcagtgttc ggttaaaatg ctcacttatg gcatttattt tcggcgtata
      121 gagataagtc catacgaacc cgacggtggc ttcctgtagc gttttgtcga attttcccga
      181 gaactctttg cccatcatca gtgatgatct ggttgatacc aacaaggaac aactgggcta
      241 cacagcagaa gaaatgcctg atgatatcac ttttaagcga tagtacggcc agagagtagt
      301 tgctgtgttc gtcgagcatg gtaatgaaat aacggtacag ctcgccattc tgagctcaat
      361 agtatccatg ccactgagct tacaggtctt tatcgggcga tagtgcttta ttcttcgggg
      421 ctttgtggat gatttttcga aatgttcatg atagttaggt aaagctaccg catatcattt
      481 gcaaaatgac agatagatat acttcaattt gaatcttaac aattagtatg ggaatgctgt
      541 caatcctctg tatgcgtcca ccacatattg aaatttttgt tctctaatat attccacggg
      601 cgtcaagagt gtgaggcctg tgctgttata cagcaccaga cgttgtcccc catctttgac
      661 ctgtctcttg atcagatctc ctgatcaaga gacttcatca ccaggtaacc ctcagccata
      721 tcctgatgtc tgaaccagcc atcccacata actacccaac cggggctgct tgtgcgtttg
      781 ctgtcatgcc atcgccccag tttagccagt ttcaaacagg cccatttcag ggtcggtgtt
      841 tgcgctggaa gctgctttcc ttccagctta acccacagta gtttccactc tgtcggcgtc
      901 agtattttct cacagctgtc attttgtgtt tcttcactga tacttccctg ccgcaggccc
      961 agcacccgca ccgcgataac caccatgcgc tcaaggttat cccgggtctg cattcgcagc
     1021 gattccacac atgtaccacc acttttccac gctttgtggt attcctctat cagccagcgt
     1081 tgctcgtaat ggctgacgat acgtcgcgca tcggcggcac tcgccacttt ttctgatgtc
     1141 agcggatacc agcaggctcc gtcctctcct gctccccgca acagacatac gtgagcgaga
     1201 gcgcctggcc gctgttgtcg ggatttttta tgctgacttc gctgtaactg atgagcatcc
     1261 gggcctggcg ggctgcccgc ccgccttttt gcatcacatt cagcgtgtgg cttcccgcgg
     1321 ctgactttat aatgcaggta atgccagata tccgcttccc ggtcacagac agtgattacc
     1381 tgtttctgta tctcccccag ccgttcggcc atgcgctccg aagcctgctg ccagcggtaa
     1441 ctttcttttt ctgtcacgaa cggtgcaata gtggttcttc cgcaacttgg gtggaagaaa
     1501 atggatgaaa ctttctggtg aacaaagatc taagctgttt taagtcatgg gcaactcaat
     1561 gcactctctt aagacacttc tacagttacc ttgcggatgg cgatgcagtc gacaaattat
     1621 tagctctgac ggtataaccc tccatctcca cggaaaacgc aaaaaagcac aatgtcctga
     1681 atgctttaag cgtagcgact ctgttcatag ttgtcgtcgg cgccggatac agcatctacc
     1741 ctgctcagga catacgctat ggcttgtatt ttccgtccgc cactggtact gccgtaaccc
     1801 ggcttgttca cgtaaaattt ttgccgggtc gcttgctccc ttcgccggtt cacaccaaca
     1861 gtcttcacag gcgttacaaa atttacaacg tcaactggga ttaatcgccg gaggtgaggc
     1921 cgggaaacgg gcagcaacgg cagtggggct ccgttgcagt gctgatactc tccttcgcag
     1981 ggttatcaat accccggaga cgaaacagtc aggcgcgcct catgtcggta ttgatgagtg
     2041 ggcgtggcat cggggccacc gttacggtac gttaatcgtc aatcttgata ctcaccgtcc
     2101 cctcgtcctg cttcccggcc gtgatcagcg tacgctggcg acctggttca gaaaatatcc
     2161 ggaaatacaa attgtctcgc gtgatcgcag tggagtctat gcgacagcag cacgtgaagg
     2221 tgcacctcag gccagacagg tggccgatcg atggcatctg ctaaaaaata ttggcgatgc
     2281 gcttgaacga atgatgtaca gacatatacc tctgatacgt cttgttgcca gtgagttgtc
     2341 accaaagaaa tcacctgatc cagaaccatc tgtgcctgca gcatcgctcc gtcgtccgga
     2401 acgcctaaaa caacaaaccc gcaaaaaacg gcatcagcgt tggacagagg ttatggccct
     2461 gcataacaag gaatgtagtt tcagggaaat atcccgtatt acaggcctgt cgcgtgtgac
     2521 agtcagtcgc tgggtgcgtt caggaacatt ccctgaaatg tcaacccgac ctccaaagcg
     2581 agggcttctg gacccatgga gggagtggtt aaaagagcaa cgagaaagta gtaattataa
     2641 cgccagccgg atatggcggg aaatggtggc ccggggattt acaggcagtg aaaccatcgt
     2701 cagggatgcg gttgccaaat ggcgtaaagg ctggaaccca ccggttacta ctgccgtcag
     2761 acttccttca gtgtcccggg taagccgctg gttgatgccc tggagaataa ccagggatga
     2821 agaaaattat gcttcccgat ttattagtct gatgtgtgaa aaagaacctg agctgaaaat
     2881 agcgcagcaa ctggcactcg agttctaccg tattctgaaa acccaaaata aatcacagct
     2941 aagcagttgg ttcaatcgag tccacgaaag cggttcagca gaatttcggc gcgtggcggc
     3001 ggggatggaa gctgatgctg cagctatatg tgaggcaatc agtagtcgct ggagtaatgg
     3061 tgttgtcgaa ggtcatgtaa atcgcctgaa gatgctgaaa cgccagatgt atggtcgagc
     3121 aggatttgaa ctactcaggc agagggtaat gagtccactg gcatgaaaat ctccacccag
     3181 gttgcggaag agccactttc cgtcgttgac actgacgaca tcttcctgct gaaacagcgc
     3241 ctggccgaac aggaagcgct gatccacgcc ctgcaggaaa agctgagcaa ccgggagcgc
     3301 gaaatagacc atctgcaggc gcagctggat aaactccgcc ggatgaactt cggcagtcgt
     3361 tccgaaaaag tctcccgccg tatcgcacaa atggaagccg atctgaaccg gcttcataaa
     3421 gagagcgata cgctgactgg tagggtgtat gacccggcag tacagcgtcc gttgcgtcag
     3481 acccgcaccc gtaagccgtt ccctgaatca ctaccccgtg acgaaaagcg actgttgcct
     3541 gcggcgccgt gctgcccgaa ctgcggcggt tcactgagct atctgggcga ggataccacc
     3601 gaacagctgg agttgatgcg tagcgccttt cgggttatcc ggacggtacg ggaaaaacat
     3661 gcctgtactc agtgcgatgc catcgtgcag gcacctgcac cttcgcggcc catcgagcgg
     3721 ggtatcgcca gaccggggct gctggcccg
//
LOCUS       DAZGJW010000276         3730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZGJW010000276 DAZGJW010000000
VERSION     DAZGJW010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.279"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            50..325
                     /gene="ypdI"
                     /locus_tag="ACHKIK_005019"
     CDS             50..325
                     /gene="ypdI"
                     /locus_tag="ACHKIK_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFO9086000.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(402..644)
                     /gene="yfdY"
                     /locus_tag="ACHKIK_005020"
     CDS             complement(402..644)
                     /gene="yfdY"
                     /locus_tag="ACHKIK_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO9086001.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            997..1917
                     /gene="lpxP"
                     /locus_tag="ACHKIK_005021"
     CDS             997..1917
                     /gene="lpxP"
                     /locus_tag="ACHKIK_005021"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO9086002.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2273..2344
                     /gene="ypdK"
                     /locus_tag="ACHKIK_005022"
     CDS             2273..2344
                     /gene="ypdK"
                     /locus_tag="ACHKIK_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO9086003.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2409..3647)
                     /gene="alaC"
                     /locus_tag="ACHKIK_005023"
     CDS             complement(2409..3647)
                     /gene="alaC"
                     /locus_tag="ACHKIK_005023"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO9086004.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT          968 a          949 c          876 g          937 t
ORIGIN      
        1 agccataaca tcgtaggttc tcagtgaacc attttcagga taactttcta tgaaagtaaa
       61 cttaatactt ttcagcttat ttttattggt ctctattatg gcatgcaatg tttttgcatt
      121 ttccatttct ggtggtgtaa gtgagaggag ctataaagag actgaaaaaa catcagcgat
      181 gacaaccaca cactctacaa aacttcagcc atcacaggcg attttgttta agatgagaga
      241 agatgcgcca ccattaaacc tcacagaaga aatcacgcct acttatccga caaaggcgaa
      301 ttatcttatt catcctgtgc gatagcgtct gtcacatggc aggtgaacaa tcatgtctga
      361 tgctgatgtg aacacctgcc caaatgagcc atcctgaccc actatcgcct gccatacccc
      421 accataaagc gaatcatcaa gccacaagcc ggggcaagaa acataaatat ccccgtcact
      481 agttcattac cgccagttaa ccacatggtg aagtaataaa tgagtgcagc gaggatcacc
      541 atgccgaaga aagaagagac cgccgatacc acgttcagta cctgaccgat gtagccgctt
      601 acgcagacaa tacacagggc gagaaacatc cataaataaa tcatagctca acctcctgcc
      661 gcgctaaaaa acctgtaact tcagtcactt taatagtaag acaatcctta acctctggca
      721 atttttgcta agcatcaatg aaaacagcat gttaaatgca agactgttgt gtacggaaaa
      781 atatttactt tgcacgatta ataatcagta gctgaaagca gtcagcgagt gataaagtac
      841 agataccgca ttcatgctcc tcgtggttat gtcctgacta gtctttacac tctttacagg
      901 aaccattgtc gtacatgatg gcccaaccaa ttgagtgtac cgctgtcgac atggaatctt
      961 ctgcgataca acaattcgta tctacagaag gtaactatgt ttccacaatg caaattttcc
     1021 cgcgagtttc tacatcctcg ctactggctc acatggtttg ggcttggtgt actctggctt
     1081 tgggtacagc ttccttatcc tgttctctgc tttctcggca cgcgtattgg cgcaatggcg
     1141 cgaccattcc tgaaacgtcg tgaatctatc gcccgtaaaa acctggaact ttgtttcccg
     1201 cagcattctg cggaagaacg cgagaagatg attgccgaaa actttcgttc actcggcatg
     1261 gcgctggtag aaaccggcat ggcatggttc tggcccgaca gtcgcgtacg taaatggttt
     1321 gatgttgaag ggttggataa ccttaaacgc gcacaaatgc aaaatcgcgg cgtaatggtt
     1381 gtcggcgtcc attttatgtc gctggaactg ggcggccgcg tgatgggact gtgccaacca
     1441 atgatggcta cctatcgtcc acataataat cagctgatgg aatgggtgca gacccgtggg
     1501 cgcatgcgct ctaacaaagc gatgatcggc agaaataatc tgcgcggcat tgtcggtgca
     1561 ctgaagaaag gtgaagcggt atggtttgct cccgatcagg attatggtcg taaaggcagc
     1621 tccttcgcgc cgttctttgc ggtggaaaat gtcgccacaa ccaatggcac ctatgttctc
     1681 tcccgtctct ctggcgcagc catgttgacc gtaacgatgg taagaaaagc ggattacagc
     1741 ggatatcgtt tgttcatcac cccagagatg gaaggctacc cgacagatga aaatcaagcc
     1801 gctgcctata tgaacaagat tatcgagaaa gagatcatgc gcgcaccgga gcagtacctc
     1861 tggatccacc gtcgctttaa aacgcgcccg gtgggagaat cgtcgttgta catttaacaa
     1921 actgctctta ctctttccct cctcaaatcg gccataataa cctcatggtt tttatggctt
     1981 ttttcttaag gttttttcct tcgctgaata tctaattatt accgtcagaa attaaactgt
     2041 cgccgatatg agacacttgt aagttactaa aagtaaataa gaaaatgtct cttgtctcgt
     2101 ttcagtttta ggcgtacatt agcgccgtct cgagcagttt agctcagaat tctggggtga
     2161 tgcccacggt aaaatacagg gcttttaagg tcgctttgcc cggtgtcaac tcactgtatc
     2221 aggtgtaatg aagtcattca ggcgtaacag taattacgcg gagagatgta aagtgaaata
     2281 tttctttatg ggcatttctt ttatggtcat cgtttgggcc ggtacttttg ccctgatgat
     2341 ctaaaagcaa actgtgcaat gaaaaacagg agccatcagg ctcctgtttg catttctgac
     2401 gacggtgttt attccgcgtt ttcgtgaata tgtttgctgc tggcgggtag taaaccgtcg
     2461 gcacggaaca tcgctttaat cccacgaatc gcctgacgaa tacggtcgcg gttttcaatc
     2521 agtgcaaagc gaacatgcgt atcaccatag tcgccaaagc caatccccgg cgagacacaa
     2581 accttcgctt cgttaaggag cttcttggca aattccagcg atcccatggc cgcatatggt
     2641 tccgggattt tcgcccagac atacatcgaa gccttcggca tttcaaccat ccagcccgct
     2701 tcatgcagcc ctttaaccaa tacatcacgg cggcgtttgt actgttcggc aatgtcgcgc
     2761 acgcactgtt gatcgccctc cagcgccgca atcgccgcca cctgcaacgg cgtaaaggtg
     2821 ccgtaatcgt gatagctttt aatacgagcc agggcgctga ccagcgtttt gttgccaacc
     2881 ataaagccga tacgccagcc cgccatgttg tagcttttcg acagcgtaaa gaactcgact
     2941 gccacatcgc gtgcacccgg tacctgcatg attgacggcg ctttccagcc atcgtagacg
     3001 atatcggcat aggccaggtc gtggaccacc agcacatcgt agcgtttcgc cagcgccaca
     3061 accttttcga agaactccag ctccacgcat tgcgcggttg gattcgacgg gaagccgagg
     3121 atcatcatct tcggtttcgg ataactttca cgaatggcgc gctccagttc gttgaagaaa
     3181 tcgacacctt ccaccagcgg cactgagcgt acctgcgccc cggcaatcac cgcgccataa
     3241 atatggatcg ggtaacttgg attcggcacc agcaccgtgt caccatgatc cagcgtcgcc
     3301 agcatcagat gcgccaggcc ctctttcgaa ccaatagtga cgatggcttc tgattccggg
     3361 tcgatttcaa cgtcgtagcg atcctgatac cagcgggaaa tggcgcgacg taaccgcgga
     3421 atgccgcgtg aagtggagta accatgcgtg tccgggcgct gggccacagt acataatttt
     3481 tcgacgatat gcggcggagt cgcaccgtcc gggttaccca tgctgaaatc gataatatct
     3541 tcgccgcgcc gacgcgcagc cattttcagt tcagcggtga tgttaaaaac gtagggcggg
     3601 agacgatcaa tgcgcgtaaa gcgacgttca gggcgagtgt cagccataat ttcctcagat
     3661 taacgttagc gcccggaccg tccgagcgac gctgccacaa tggtggctgt tttgaaaata
     3721 gcctgattaa
//
LOCUS       DAZGJW010000277         3712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZGJW010000277 DAZGJW010000000
VERSION     DAZGJW010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.280"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1720)
                     /gene="rsxC"
                     /locus_tag="ACHKIK_005024"
     CDS             complement(<1..1720)
                     /gene="rsxC"
                     /locus_tag="ACHKIK_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO9086005.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQ"
     gene            complement(1713..2291)
                     /gene="rsxB"
                     /locus_tag="ACHKIK_005025"
     CDS             complement(1713..2291)
                     /gene="rsxB"
                     /locus_tag="ACHKIK_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO9086006.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(2291..2872)
                     /gene="rsxA"
                     /locus_tag="ACHKIK_005026"
     CDS             complement(2291..2872)
                     /gene="rsxA"
                     /locus_tag="ACHKIK_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO9086007.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAIGFSLVMVLFAAIRERLAV
                     ADVPAPFRGHAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(2949..3389)
                     /locus_tag="ACHKIK_005027"
     CDS             complement(2949..3389)
                     /locus_tag="ACHKIK_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO9086008.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(3475..3690)
                     /gene="ydgT"
                     /locus_tag="ACHKIK_005028"
     CDS             complement(3475..3690)
                     /gene="ydgT"
                     /locus_tag="ACHKIK_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO9086009.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
BASE COUNT          955 a          990 c          943 g          824 t
ORIGIN      
        1 gctgttccag cttgcgcgct ttggcgcggg cgatagctgc ttcaacggca gttttacgtg
       61 gatcagcaac ggttgctgcg tcgttagttt gctgcagttc tgcctgtttc gctctggctt
      121 gcgctttacg ggcttcccgt gctgcaataa ttgcactgtt atccgggcgt tcgcccgctt
      181 taatcacaat aggctgtgta gcctgggcct gtttctcttt cacccgcgcc agagcggcag
      241 caatcgcatc tttatctttg gctgcaggtt gaacggctgc gctcttatgt cgttcaaggc
      301 gagccgcttt ttcgcgctcc agacgagcct ggcgcgcttc gaaacgcgct ttggcttctg
      361 cggcgcgctt ttcttcctga cgaatagccg caatttcagc tttttcctga cggaaatatt
      421 gcaccagggg aatattgctc gggcaaaccc acgcgcaagc cccacattca atgcaatcag
      481 caatgttatg cgtggtagct ttatcgtgtt gctgaccttt gctgaaccag tacaactgtt
      541 gcggcaaaag atccgcaggg caggcgtcag cacaggcgct acaccggatg cagctttgtt
      601 cttcctgtgg ttcgccaagt tcattggcag agggagccaa cagacagttg gtaatcttta
      661 cgaccgggac atccagccat ggcaaggtaa agcccattag cgggccaccc ataatcacca
      721 tttgatcggc agaggggcag aatccggcat cattcaataa atgacgcact ggcgtcccca
      781 gccgtgccca gacgttgccc gggcgagcga ttgcttcgcc agtcagggtt acaacacgct
      841 cggtaatcgg ctcgccatca ataacggcac gtttcactgc ataagcagtg ccgacgtttt
      901 gcattaatac gccgatatcg gatgaacgcc cgccatgtgg aacctgcttc ccggtcagaa
      961 tgtaggttaa ttgtttagca ccgccagaag gatatttggt tggaatcacc cgcagagaaa
     1021 tatcgttaga gtccgccagc accgcgcgca gcatggaaat cgcctgcggt ttgttatctt
     1081 caatgccgat aagaatttcg cgtggctgca gaatatgcgc aagaatgcga ataccctcta
     1141 cgacctgagc cgcgcaatcc tgcatcaaac ggtcatcggc ggtaatgtac ggctcgcact
     1201 cagccgcgtt gataatcaac gtttcaatct tatctccgcc accctgcaat ttaacgcctg
     1261 tcgggaatcc tgcaccgccc agcccggcaa caccaaactg atgtatgcgc tcgattaact
     1321 cttcgcgact gcgagtgcga taatcggccc agccgtcgcg cgggatccag cagtcttcac
     1381 catcggcatc aataatcacg cttaattcag ctaaagctga aggatgagcc gtagagtggg
     1441 gcgcaatagc cgtaacggta cccgaggtgg gcgcgtgaac aggcagcatt ttgccgcgac
     1501 cacgggtaag cggctggccg cgcaatactt tatcgccgac gctaacgcac aactcacctt
     1561 cagcgccaat atgctgtttc agtggaataa caaaacgctg cgccagtggt acctggcgca
     1621 ggggtgtacc gttggactgg gttttcatct ccggtggatg gatgccgccg ttgaaatccc
     1681 agattttatt ttttctgaat gcagagaata acttaagcat ggtgttccac gggaatgata
     1741 cgcacgggaa tggtgttcag atcccatttc caggagtcag gtgtttctgc gaccggttgc
     1801 aacgagatgc agtgcgtcgg gcacggatca acacataaat tgcagcccgt acagagatca
     1861 ctcattaccg tatgcatggc acgggtagcg ccaacgatgg cgtctaccgg acacgcctga
     1921 atacatttag tgcagccaat acagttattt tcatcaataa ccgccaccat ccgcgcaggc
     1981 gttatctctt gcgcttcgcc atccagcggc tgcggctcga cattaagcaa ctcggcaatt
     2041 tttagcatca cagcttcgcc acctggggcg caacggttga ttttttcacc gttacagctg
     2101 atggcttccg cgtaggggcg acagccggga taaccgcact gaccacactg gctctgcggt
     2161 aagatttcgt caattttctc aacgaccgga tcgtcttcca ccgcaaaacg gcgggaggca
     2221 taacccagaa tggcgccaaa cgccaggccc agcaggctca cggcggcaac ggcaatccag
     2281 atagcattca ttacaacttc accaaaccac taaagcccat aaaggccaga gacataagac
     2341 ctgcggtaat taacgcaatg gcatgaccgc gaaaaggtgc cgggacatca gccacagcaa
     2401 ggcgttcgcg gatggcggcg aagagcacca tcaccagcga gaaaccgata gcggcggaaa
     2461 aaccgtacag cgccgactgc aagaaattgt gcccgagatt gatattcagc aacgccacgc
     2521 cgagcactgc acagttggtg gtgataagcg gcaaaaaaat ccccagcaga cggtaaagca
     2581 ccgggctggt tttgcgcacc accatctcgg tgaactgcac gaccacagca atcaccagaa
     2641 taaatgccag ggtgcgcagg taaatcagat taagtgggat caaaatccac gtatcgataa
     2701 gccaggcgca aatagacgcc agcgtcatca caaacgttgt tgccagcccc atgcccatcg
     2761 cggtttccag ttttttggaa acccccataa acggacagag accgagaaac ttgaccagta
     2821 caaagttatt gaccagtaca gttccgacaa acagtaacag gtagtcagtc atttttcggc
     2881 ctgaaataaa aaagccgcct attatccgtt aatccagagc aggcgacaac aggttaactg
     2941 taaggttatt acggattcac aaacgtcgct tttacccgcg acgagcgttt gaaatagggg
     3001 acgatcaatg cggttgccaa caaagtaaac agcaactggc gaacggctat gccatcttcc
     3061 acgggtgaaa aagcgaatgc ttttactgcc agcaatacgg aaataagcag ccagataata
     3121 tagtgtttag gaacacaacg ccgacgttta aagaaagcga tggtcaacca cagtgtgtaa
     3181 taccacatgg cgatagcggt aataaaagaa acgccccata agatttgtgt cgtcagtgct
     3241 tgtccgccga gcgtttgaaa tgtttgcgga gaagataacg cagcggtata cagcaacaac
     3301 gccagcgtcg tactcaacag agcgactaaa agccaggcca acgggccaag caaccagcct
     3361 ccaatacgtt gtggcgttgt ggtggtcata ggttctccag aatagcgacg caaacaggca
     3421 aatctaattt taggggcgtt attataaggc ttttataacc gatcgctact ttctttattg
     3481 gacatagtgc cagacggact tcggtacctg gccgaggtca aacaaacgcc cgccagaaac
     3541 cagctctgcg cgacggtgat cggcagcacg atacatattg atcagttcct gatcgtccgt
     3601 cagggtgtaa ttaagatggt cgtagagttt ttccagactt tcgagtgaac tgattttgcg
     3661 aaattttaat aagtagtcct gaacagtcat aaaaggtcca taaaataatt aa
//
LOCUS       DAZGJW010000278         3630 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZGJW010000278 DAZGJW010000000
VERSION     DAZGJW010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.281"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            318..839
                     /locus_tag="ACHKIK_005029"
     CDS             318..839
                     /locus_tag="ACHKIK_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO9086010.1"
                     /translation="MSHDAEKKIRLCIQCENEALKDSDFCAVCEEREFKKIRGWLYLP
                     AIGLVLTILANMISINFTIRFLMENIAQIGHAQKGILFFELFAFIGMFSYGIFVSSLF
                     FRKKRQLPHHYIALVGIGTIFVAVDLLLGHIYLDVPYVFDTVKPLVRNVFSACIWIPY
                     FIVSERVKRTFVK"
     gene            874..2007
                     /gene="cdiA4"
                     /locus_tag="ACHKIK_005030"
     CDS             874..2007
                     /gene="cdiA4"
                     /locus_tag="ACHKIK_005030"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDI system deoxyribonuclease CdiA-o11"
                     /protein_id="HFO9086011.1"
                     /translation="MVNATLSVVQKNSAFVGSATGELAARAIGMLYPGVKQSDLSEEQ
                     KQTISTLATVSAGLAGGLTGSSTASAAVGAQSGKNAVENNYLSTNQSLTFDKELSDCR
                     KSGGNCQDIIDKWEKISDEQSAEIDQKLKDNPLEAQVIDKEVAKGGYDMTQRPGWLGN
                     IGVEVMTSDEAKAYVQKWNGRDLTKIDVNSPEWTKFAVFASDPENQAMLVSGGLLVKD
                     ITKAAISFMSRNTATATVNASEVGMQWGQGNMKQGMPWEDYVGKSLPADARLPKNFKI
                     FDYYDGATKTATSVKSIDTQTMAKLANPNQVYSSIKGNIDAAAKFKEYALSGRELTSS
                     MISNREIQLAIPADTTKTQWAEINRAIEYGKSQGVKVTVTQVK"
     gene            2007..2510
                     /gene="cdiI4"
                     /locus_tag="ACHKIK_005031"
     CDS             2007..2510
                     /gene="cdiI4"
                     /locus_tag="ACHKIK_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDI system immunity protein CdiI-o11"
                     /protein_id="HFO9086012.1"
                     /translation="MAFNKDQDYWANIFVTPDFLSVETYSGLGMTGRDPLFSPRLLQP
                     DVDDKSLGEEILQALSDSRTLDVLEERVAFFDLEKSKEQYAAWIATLMEKYGYRTKRA
                     LFKNMKKVGIHLVNDVITIRPSFHEKLEAWSGNRINESDYVVLPADSSPTEIGSGLRL
                     ALSRCKG"
     gene            complement(2907..3257)
                     /locus_tag="ACHKIK_005032"
                     /pseudo
     CDS             complement(2907..3257)
                     /locus_tag="ACHKIK_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3278..>3630)
                     /locus_tag="ACHKIK_005033"
     CDS             complement(3278..>3630)
                     /locus_tag="ACHKIK_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9086013.1"
                     /translation="KMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENA
                     LRAVSLGRKNFLFFGSDHGGEREALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNR
                     VSELLPWRIALPAE"
BASE COUNT          985 a          770 c          932 g          943 t
ORIGIN      
        1 attggtattt ggataaaagc aaagttacaa gaaagaaaag acgataaaaa ataggcttac
       61 ggtaacttca actttgggga atttttattg gtagttatgt tcttggtcaa tgccatcagg
      121 aatgaggtaa tgtgcgtatt accgtggaag tgatcggtga gtaactccaa tctcaataga
      181 ggttattggg cgctctcctt ccatgaaaac acgcaaccta atcggggacc ggggcatatg
      241 ctgactggtg acaatacaga tgaaaataaa cagcatgatc ttcaactttt cccgtataaa
      301 taataggtga ttatcagatg agccatgatg cagagaagaa aataagactt tgtattcaat
      361 gtgagaatga agcactgaag gacagtgact tttgcgctgt ttgtgaagaa cgcgaattta
      421 aaaaaattcg tggttggcta tacttgcctg caataggact ggttttaaca attctcgcta
      481 atatgatctc tattaatttc actatacgat tcttaatgga aaatattgcg cagatcggtc
      541 acgcacaaaa ggggattctc ttttttgaac tctttgcttt cattggtatg ttttcctatg
      601 gaattttcgt tagtagtttg ttttttcgta aaaagcgtca attgcctcat cactatattg
      661 cacttgttgg gataggaacg atatttgtcg ctgtcgattt actgctgggc cacatctatt
      721 tagacgtgcc ttatgttttt gatacggtaa aaccattggt acgtaatgtg tttagtgcct
      781 gtatttggat cccctatttc attgtatctg agcgcgttaa gcggacattt gtgaaataat
      841 ttgtccgtca gatgtcccac atgaactcat gcagtggtga atgccacgct gtcggtggta
      901 cagaaaaata gcgcctttgt cggctcagca acaggagaac tggcagccag agcgatcgga
      961 atgctgtatc cgggcgtgaa acagtcagac ctcagtgaag agcagaagca gacgataagt
     1021 acgctggcaa ccgtgtcagc agggctggcc ggcggtctta ccggaagcag caccgcatca
     1081 gcagcagtgg gagcacagag cgggaagaat gctgttgaga ataattacct tagcacaaac
     1141 cagtctctga ccttcgataa agaattgtct gattgtcgga aatctggagg taactgccag
     1201 gacattattg ataaatggga aaaaatcagt gatgaacaga gtgcagaaat cgatcagaag
     1261 ctgaaagaca atccgctgga agcccaggtt attgataagg aagttgcgaa gggcggatat
     1321 gatatgacgc agcgcccagg ttggctgggt aacattggtg tggaagtaat gaccagtgat
     1381 gaagccaaag cctatgttca gaagtggaat ggacgggatc tgacaaaaat agatgtaaac
     1441 agccctgaat ggactaagtt tgcagtgttt gcttctgatc ctgaaaatca ggccatgctg
     1501 gtcagcggag gtttgctggt aaaagatata actaaagcgg ctatatcatt catgagtcgc
     1561 aatacagcga cagcgacggt caacgcatca gaagttggaa tgcagtgggg acagggaaat
     1621 atgaaacagg gtatgccgtg ggaggattat gttgggaaat ctcttcccgc tgatgcccgt
     1681 ttaccaaaga actttaagat ctttgattac tatgatggtg ctaccaaaac tgctaccagt
     1741 gtaaaatcaa tagatacgca aactatggcg aaattggcta atccgaatca ggtttacagt
     1801 tcgataaaag gaaacatcga tgctgcggct aagtttaaag aatatgcact ctctgggcga
     1861 gaattaactt catcaatgat ctctaacagg gagatacagc tcgctattcc tgctgacaca
     1921 actaaaacac agtgggcaga aataaacagg gctattgagt acggtaaatc tcagggcgtc
     1981 aaagtgacgg taacgcaggt gaaataatgg cttttaataa agatcaggat tactgggcga
     2041 atatatttgt tacaccagat tttttgagtg ttgaaactta ttcaggatta ggaatgacag
     2101 gcagagatcc attgttttca ccacgtttat tacagcctga tgtagatgat aaaagcctag
     2161 gtgaggagat cctacaggca ttgtctgaca gccgtacact agatgttttg gaagagcgag
     2221 tagctttttt cgatcttgaa aaaagtaaag aacaatatgc agcttggata gctactctga
     2281 tggagaagta tggttataga actaaacgtg cattatttaa aaacatgaaa aaagttggta
     2341 tccatctggt aaatgatgtt atcacaatcc ggccttcatt ccatgaaaag ctggaagcat
     2401 ggagtggtaa caggatcaat gaatctgact atgtggtgct tcctgcagac agttccccaa
     2461 ccgaaatcgg ctcaggtctt cgcctggcct tgagtcgctg caaaggttaa cctagatatc
     2521 ccgcccatca ctggcgggat ttttgtgtgc ttccaagcat atcatcttga cgcgtttttc
     2581 gggcgcgtgt catcggcggc gcatacgaat gatcgcgatc tgacgatggt tgatttattt
     2641 tgttgcgctg gggcgatcgc cagacaggta ccggaggcgg gctctgaagt actgagtagg
     2701 gtgatcgcgt caggccgcga agtactgact cggggattaa tggcaggtga tgatgtactg
     2761 acccgggaat gactccgggc tgattcaggt tcttattcgc tgtcagcccg tgtggttgct
     2821 ggtctggctc atttcctgtc gtcaggagtt caactcccga ctgcaggtta tggcccggtg
     2881 cacgaacacc tgacggcagg tatcactcac ccggagaagg taaatccctc aagaggcagc
     2941 aactctgcca gtcgctcctc cggccactcc ggcagacgca tcaggacgtc tgtcagccag
     3001 gcatgcggct ccagaccgtt gcgtttcgcg gtttccagca agctcattat ttgcgcggcg
     3061 cgttctcctg ccatctgcga accggcgaac agccacgatt ttctgcccag gaccacgttt
     3121 ttgatggccc gttcacacac attattatcc agcggcaccg caccatcttc caggaagcgg
     3181 cttagttcca cgcgatgaga cagcgcatag acaatggctt tgtgtaatgc ccttcccgga
     3241 gtaagcgtac agcgagggcc gtattgacgg ggatgtgtta ttcagctggc agtgctatgc
     3301 gccacggaag cagttcgctg acccggttga ccggccagtc tgctatgacg ccaagcacat
     3361 ggcgaaggta gctttctgga tccacgtcat tcagtttgca cgtcccgatc aggctgtaca
     3421 gtagcgcttc ccgctcacca ccatggtcag agccgaagaa caggaagttt ttacgaccca
     3481 gactgaccgc ccgcagggca ttttcagcga tgttgttgtc gatttccacc cagccatcgt
     3541 tcgcatagta cgtcagtgcc ggccactggt taagtgcgta cgcgaacgcc ttcgccaact
     3601 ctgagtgtcg cgacagggtc ttcatctttt
//
LOCUS       DAZGJW010000279         3621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZGJW010000279 DAZGJW010000000
VERSION     DAZGJW010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.282"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            268..1233
                     /gene="dusB"
                     /locus_tag="ACHKIK_005034"
     CDS             268..1233
                     /gene="dusB"
                     /locus_tag="ACHKIK_005034"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO9086014.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1259..1555
                     /gene="fis"
                     /locus_tag="ACHKIK_005035"
     CDS             1259..1555
                     /gene="fis"
                     /locus_tag="ACHKIK_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO9086015.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1641..2525
                     /gene="yhdJ"
                     /locus_tag="ACHKIK_005036"
     CDS             1641..2525
                     /gene="yhdJ"
                     /locus_tag="ACHKIK_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO9086016.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            2609..2788
                     /locus_tag="ACHKIK_005037"
     CDS             2609..2788
                     /locus_tag="ACHKIK_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO9086017.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2791..3453)
                     /gene="acrS"
                     /locus_tag="ACHKIK_005038"
     CDS             complement(2791..3453)
                     /gene="acrS"
                     /locus_tag="ACHKIK_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO9086018.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT         1011 a          816 c          834 g          960 t
ORIGIN      
        1 aatttgccgg ttatttacgc aaatttgcgt gccaaaattt tcattcataa agaaaaattg
       61 agaacttact caaatttctt tgagtgtaaa ttttagtcac tattttctaa tatgatgatt
      121 tttatgagta attatcgcac cacgctcatt ttaaatgcaa ttctttgatc catctcagag
      181 gattggtcaa agtttggcct ttcatctcgt gcaaaaaatg cgtaatatac gccgccttgc
      241 agtcacagta tggtcatttc ttaactcatg cgcatcggac aatatcagct cagaaatcgc
      301 ctgatcgcag cgcccatggc tggcattaca gacagacctt ttcggacgtt gtgctacgag
      361 atgggagccg gattgacagt atccgagatg atgtcttcta acccacaggt ttgggaaagc
      421 gacaaatctc gtttacggat ggtgcacatt gatgaacccg gtattcgcac cgtgcaaatt
      481 gctggtagcg atccgaaaga aatggcagat gcagcacgta ttaacgtgga aagcggtgcc
      541 cagattattg atatcaatat gggttgcccg gctaaaaaag tgaatcgcaa gctcgcaggt
      601 tcagccctct tgcagtaccc ggatgtcgtt aaatcgatcc ttaccgaggt cgtcaatgca
      661 gtggacgttc ctgttaccct gaagattcgc accggctggg caccggaaca ccgtaactgc
      721 gaagagattg cccaactggc tgaagactgt ggcattcagg ctctgaccat tcatggccgt
      781 acacgcgcct gtttgttcaa tggagaagct gagtacgaca gtattcgggc agttaagcag
      841 aaagtttcca ttccggttat cgcgaatggc gacattactg acccgcttaa agccagagct
      901 gtgctcgact atacaggggc ggatgccctg atgataggcc gcgcagctca gggaagaccc
      961 tggatctttc gggaaatcca gcattatctg gacactgggg agttgctgcc cccgctgcct
     1021 ttggcagagg ttaagcgctt gctttgcgcg cacgttcggg aactgcatga cttttatggt
     1081 ccggcaaaag ggtaccgaat tgcacgtaaa cacgtttcct ggtatctcca ggaacacgct
     1141 ccaaatgacc agtttcggcg cacattcaac gccattgagg atgccagcga acagctggag
     1201 gcgttggagg catacttcga aaattttgcg taaacagaaa taaagagctg acagaactat
     1261 gttcgaacaa cgcgtaaatt ctgacgtact gaccgtttct accgttaact ctcaggatca
     1321 ggtaacccaa aaacccctgc gtgactcggt taaacaggca ctgaagaact attttgctca
     1381 actgaatggt caggatgtga atgacctcta tgagctggta ctggctgaag tagaacagcc
     1441 cctgttggac atggtgatgc aatacacccg tggtaaccag acccgtgctg cgctgatgat
     1501 gggcatcaac cgtggtacgc tgcgtaaaaa attgaaaaaa tacggcatga actaattcag
     1561 gttagctaaa tgcttgatta aaaaggcgct actcggcatg gggaagcgcc ttttttatag
     1621 gtgtcacaaa gggagtgacc atgagaacag gatgtgaacc gacccggttt ggtaatgaag
     1681 ctaagaccat tattcacggt gatgcgcttg ccgaacttaa aaagatcccc gccgaaagtg
     1741 tcgatctgat ctttgccgac ccaccatata acatcggtaa aaattttgat ggtctgatcg
     1801 aagcctggaa agaagatctg tttatcgact ggctgtttga agtgattgca gagtgccacc
     1861 gcgttctgaa aaagcagggc agcatgtaca tcatgaacag cacggaaaac atgcccttta
     1921 tcgatctcca gtgccgcaag ctttttacca tcaaaagtcg catcgtctgg tcatatgaca
     1981 gttctggagt acaggcgaaa aaacactacg gctccatgta cgaacccatc ctgatgatgg
     2041 tgaaagacgc aaagaactac acattcaacg gtgatgctat tctggtagaa gccaaaaccg
     2101 gatcgcagcg cgcgttgatc gattatcgca aaaatcctcc acagccatac aatcatcaaa
     2161 aagtaccggg taacgtctgg gattttccgc gcgtgcgtta tttgatggat gaatatgaga
     2221 accacccgac gcaaaaaccg gaagccttac tgaaacgcat tattctcgcc tcttccaacc
     2281 caggcgatat cgttctcgac ccgtttgccg gtagctttac taccggtgcc gtagccgtct
     2341 ccagcgggcg aaaattcatt ggtattgaga tcaacagcga gtacatcaaa atggggcttc
     2401 gacggctgga tgtcgcgtcg cattactctg cggaagaact ggcgaaagtg aaaaaaagaa
     2461 agaccggcaa cctgtcaaaa cgaagccggt taagcgaagt tgaccccgat ctcattgcaa
     2521 agtaaaggaa gtgtaagcct ggttttcaga ttattcattt cgtgtatatt tcctgccaga
     2581 cttggttaaa catacacagg ctcaggtaat gattcgcaag tattggtggc tcgtcgtttt
     2641 cgctgtcttc gtttttctgt ttgatacttt actgatgcag tggattgaac tgctggcaac
     2701 agaaacagac aaatgccgca atatgaactc agttaatcca ctaaaactgg ttaactgtga
     2761 cgaactgaat tttcaggaca gaatgtgaat ttacatgaca cttaattcat tcgtttgatg
     2821 aattaatttc gttatgtttt catctggcat gaacattctt aatacgttat cgaccagagc
     2881 gggggcttgt ttataaagat cataacccgc catattcatt aaccagtttt gaacaattcc
     2941 gctgaaggca ccattaataa taatcatcac aacatctaaa tcgaggttat ttgctacaca
     3001 accttgttgc tgacacgcct gcaatacttc gcggagagtc tgcggattga agcccatctt
     3061 ttcgcgtatc actccctcgg ccagcatctc atcattaaat tcacatttgt gatataagat
     3121 tttcagcaac gcctgctggc ggggaatttt ggcaatatat tgcaagccga caatcaattt
     3181 ttcacgcaat tgttgaaacg ggtcatgctc taatccagcc gtcaagtgtt cctggattaa
     3241 ctcccgcaat gaaggctgtt gcaaccacat ctcattaaac agttgagtct tgttttcgaa
     3301 gtgccagtag atagcgccac gcgtaacgtt agcagcgtcg gcaatgtcgt tgagtgtcgt
     3361 cttgcttacg ccatgctgcg caaactgggc gatggcagtt tcaatcagtt cttgccgggt
     3421 cttcagagct tcggctttgg ttctttttgc catgattaat tattcaggaa ataaatatat
     3481 tcgacacaga gtgagaaaat agcgaaggtt aatctatcac ctaatgtgta tttatacgag
     3541 aggctaatat tgagttgcta taaatcgtca aataaataat atatattatt tacctaaaat
     3601 acattcgcca catgaatata t
//
LOCUS       DAZGJW010000280         3605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZGJW010000280 DAZGJW010000000
VERSION     DAZGJW010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.283"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..348
                     /locus_tag="ACHKIK_005039"
     CDS             <1..348
                     /locus_tag="ACHKIK_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFO9086019.1"
                     /translation="LFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSR
                     KGNCYDNAPMESFWGTLKNESLRHYRFKSRNEAISVIREYIEIFYNRQRRHSRLGNIS
                     PTAFREKYHQMAA"
     gene            674..1059
                     /locus_tag="ACHKIK_005040"
                     /pseudo
     CDS             674..1059
                     /locus_tag="ACHKIK_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942659.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
     gene            complement(1084..2114)
                     /locus_tag="ACHKIK_005041"
                     /pseudo
     CDS             complement(1084..2114)
                     /locus_tag="ACHKIK_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            2216..2434
                     /locus_tag="ACHKIK_005042"
                     /pseudo
     CDS             2216..2434
                     /locus_tag="ACHKIK_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942659.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            2737..>3605
                     /locus_tag="ACHKIK_005043"
     CDS             2737..>3605
                     /locus_tag="ACHKIK_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcdB toxin N-terminal helical domain-containing
                     protein"
                     /protein_id="HFO9086020.1"
                     /translation="MNVGTKNTNDVTKQVLQRISERKKTYFLEKNRKIKHLSVDDIIS
                     IGGIIQSEIRRTLHPSPLPLPATTSARNWKSVIIHYIGLLISKPLQMPFFSYNLQQES
                     KDVTSLPNHIRGNNNEKLIPTGIKEHPEYVRENNIQTQVIHKREKRNTNNIKKTEELI
                     QAKEKDTKYSLIVDNIKYKIFGHVDTLPPKDKELIKILKDEIKNYTGLPEKNSRKGIE
                     SLKNQVRILEKLRADASPAVQDNISEIITSLHNEYKSLKVEIDKKIHVIWIAGAPPET
                     ITKYAKAYKAAYP"
BASE COUNT         1122 a          790 c          796 g          897 t
ORIGIN      
        1 ctgtttatgg cgctcaggag ccagcgccca cctgccgggc taatccacca ctccgatcga
       61 ggttcacagt actgcgcata cgattaccgg gtcatacagg agcagtttgg tctgaaaaca
      121 tcaatgtcgc gtaaaggtaa ctgttacgac aacgctccga tggaaagctt ctggggaacg
      181 ctgaaaaatg agagcctgag gcactatcgt tttaagagcc ggaatgaagc catctcagta
      241 atacgggaat acattgagat tttctacaat cgtcagcgtc gtcactctcg tctggggaat
      301 atctccccga cagccttcag ggaaaaatat catcagatgg ctgcttaaaa aaagaacaag
      361 tggtagtgtc cgctattgtc agtacacctc agtggtagtt tccttatatg aaatcagtca
      421 gcaaaaaacc gacgagcccc ctcgcccaat tcgggagggg aggaaaagtg gaaaagcccg
      481 aagggcttaa aaaaatcatt gcggtattag cataaaaagc ctgtatcaat gagcagtgac
      541 tatgttaaga acggcaaaag tacatatcta cccgacatcg gaacaggcag agtaagtatc
      601 tcaatgctcg gttcggcgca gtacattttg cgtacaataa agatcttcac atcaaaaaaa
      661 cacgcttacc tgcgatatgg agcttgagca ctatgccatc atgtctaatg gggaaaaaag
      721 ttccgaatcc tcgtcatctc atcaatgcct accgcaatct gcgacgaaaa caaaaagcgt
      781 tatcccgcaa gcgcgaaccg taaaaaggcc agaatccgtc tggcagactt acacgaacgg
      841 gtagcgaatg ctcgtgctga ttttcagcat aaactttctc gtacaattgt tgacgaaaac
      901 caagcggtaa ttgttgagac gctgaaaaca gccaacatga tgaaaaacca caatctagca
      961 agagcgatag gtgatgcagg ctggcatagc gtcatcacaa cgctggagta caaagctgcg
     1021 ggaagaggca ttcacctagt aaaactcgac caatggtttg ataaataact gatcctaata
     1081 cagctacact ttagcctgtc catggtttcc tccagggaat ggtctgacgt tttccataaa
     1141 atggcgaacc tttttcagca gttgccacat tgaacggcac tggtgatttc gggtgattgt
     1201 ctcatgaagt gtctgccaca accgttcgac atgattgacc tacggcgagt aaaccggctg
     1261 ataaatgccc gtgaactttg ggtttgcttt cagccagcgc aacgtttcac ggcttttgtg
     1321 gatgatgtaa ttatcaacaa tgagcgtaat cgttttttcc caccggtatg tcgctttcag
     1381 atttttaaca gaatgataaa taaggacgaa cctttactgt ttccaccaac gtagctcact
     1441 tttcccgtac cgctgtgcag cgcaccggca agatagtact tttcattctg gcccggcgtc
     1501 acaacgcgct tttgctgccc acgtaactgc cagtctgcgc caattttggg attgaggtga
     1561 atatccactt catcatcgta aaacacgggg ttttccgcac tgcatttcgc cagtgcttca
     1621 tggatagcga ccattttttc ttctttgtgt gggtcacgga tacacagtgt tggcacggtt
     1681 ctctaccata ccaatccggc agctggcaac cattgccgga tggttgaaga atgtagtgga
     1741 caatcggtga catcacgggt ttttatcgcc agcagttcgg tactccagcg ggaacgctga
     1801 tgaccgaaat cgccgggaga atgcttaaca agctcacgca gcaatgcgca aatatgttcg
     1861 aacggccaac gtctcccacg tcctgatggt aatgacttca ggccttcaac accagacagc
     1921 gtgaaccagt taatccagcg accaatggat gagcgggcac tgcagagcgt tctgacaaca
     1981 tgactgacgg tttaaccacg atgcagcatc agcatagcgg tgagccgccg ggcgtgattt
     2041 ttgtcctttg ttttatggat agttttctgc atcagacgtc gttcgattct gggtattagt
     2101 gctatgatcg tcattgcgca gtccggtggg tgatttggga tattcagcga ttgatcagat
     2161 cgcataaacc gggctgagtc cccccggagt aatctactat tccgaacagt tatttagttc
     2221 gaaaaggtgc cattgttgtg gttacaaaat gccggaaatg ccgtttcata aacgcatctg
     2281 gcgatgccct gaatgttgag ctgagcacga ccgcgatatc aatgcggcac tcaacatccg
     2341 gcaaaaagga atactggaac taaaggcggc gggactcgtc gcctctgcct atggagggca
     2401 gcatagatcc gtcgtacaga cagttgcggc ctgagaagtg ggaagcctcg cctgacaggg
     2461 cggggagtag tcacataaag actcatcctt actctgctct ttcttcccgg gcataagcat
     2521 catgcgctga cgcttatggt tcaggaaata tccagaaata cggatggtcg cgagtgttcg
     2581 tagcgatttc atcacacacc gaaaaaaata gcacctcgtt tttctataaa aggaagtgga
     2641 ttactggtcg atgattcact tatatttccc cgctgtcaga aaatttaccg atattcacat
     2701 agaaagtata cgtgataaat tatataggag gtcactttga acgtaggtac taaaaataca
     2761 aatgatgtca caaaacaagt tctccagaga ataagtgaaa gaaaaaaaac ctattttctt
     2821 gaaaagaata gaaaaataaa acacttgtct gtagatgaca taatttctat aggtggaatt
     2881 attcagagtg aaataagaag aactctgcat ccttcacctt tacctttacc tgcgaccaca
     2941 tctgccagga actggaagtc ggttattatt cattatatag gtttgttaat atctaaacct
     3001 ttgcagatgc catttttttc ttataattta cagcaggaat caaaagatgt aacttcgtta
     3061 cctaatcata tacgaggaaa taacaatgag aaactaatac ctactggtat caaagaacac
     3121 cctgaatatg taagagaaaa taatatccaa acgcaagtga ttcataaaag ggagaagaga
     3181 aatacaaaca acataaaaaa aacagaggag cttattcaag ccaaggagaa agacacaaaa
     3241 tattcactaa tagtggataa cataaaatat aagatatttg gtcatgttga tactttgccc
     3301 cccaaagata aggaattaat aaaaatactt aaggatgaaa ttaagaacta tacaggtcta
     3361 ccagaaaaaa acagcagaaa aggcattgaa agtctaaaaa accaagtacg tattttggaa
     3421 aaacttcggg cagatgcatc tccagcagtt caagataaca tatctgaaat aatcacctca
     3481 ttgcataacg aatataaatc acttaaggtg gaaatagata aaaaaattca tgtaatttgg
     3541 atcgctggcg caccacctga gactataaca aaatatgcaa aagcatataa agcagcctat
     3601 cctga
//
LOCUS       DAZGJW010000281         3598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZGJW010000281 DAZGJW010000000
VERSION     DAZGJW010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.284"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..748)
                     /locus_tag="ACHKIK_005044"
     CDS             complement(<1..748)
                     /locus_tag="ACHKIK_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFO9086021.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMT"
     gene            complement(779..1129)
                     /gene="tnpB"
                     /locus_tag="ACHKIK_005045"
     CDS             complement(779..1129)
                     /gene="tnpB"
                     /locus_tag="ACHKIK_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9086022.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(1126..1551)
                     /locus_tag="ACHKIK_005046"
     CDS             complement(1126..1551)
                     /locus_tag="ACHKIK_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9086023.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            1605..2356
                     /locus_tag="ACHKIK_005047"
                     /pseudo
     CDS             1605..2356
                     /locus_tag="ACHKIK_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          877 a          950 c          938 g          833 t
ORIGIN      
        1 gagtcattaa ctgacaacat gcgtcaaccc agttggagag taatgcccgg ctcagttcga
       61 tgccctggcg ggcaaagatt tcactctgac gatacagtgg caggtgttcg cagtattttc
      121 ccgttaacac gcgggcaagt aatccggagc ccgcgatgcc gcgctctatc gggcgggacg
      181 gcgctggcgc ttcaactata cagtcacatt ttgtacaggc tttttttacc cgaacagtgc
      241 ggatcacttt cagggcgcta ctcaccagtt ccagctgctc agcactgact tcccccagat
      301 aatccagctc actgccacac tccgggcaac aactttcttc aggctccaga cggtgtattt
      361 cacggggaag atgtgccggt aacggacgac gatgacgtga ctgtcgcaac tggcggggaa
      421 cctgcgggtc atcctcacgc ccactgtaac gatcgctttc ctgttcgcgt tgtttcagtt
      481 gggcctcagc ctgttcaacc tcacgctgca gtttttcaga acgggtaccg aacaacatcc
      541 ggcgcagttt ttctatctgg gccctcagat gttctatttc ccgctcctcc tcttcgatct
      601 tttcctcggc acgtgccagt gcagagcgca ggaaggcctc cgtctcttca accagactca
      661 gttgctgatc tttctgacgg agggcttcag cctgctcaga gagcagcctt tccagctcag
      721 tgatacgaat gaggtatttc cgactcatga ccgtttttat aatccggcca tgacattttt
      781 acaacattgt cagtgcatta aggcgggatg ttttgggctg acgccagtcc agtttatcga
      841 ggagcattgc cagctgcgag cgggtaatgg ataccttacc gtcacgcacc gcaggccaga
      901 taaactggcc ttcctccaga cgtttggtga acaggcacag accatcagca tcagcccaca
      961 gaattttaat cgtgtcaccc cgtcggccgc gaaagataaa caggtgaccg gagaaggggt
     1021 tctcatccag cacatgttgt acctgttctc ccagaccgtt gaaggattta cgcatatcag
     1081 taacgccggc aaccagccag attcgagtgc ctgatgggag cgagatcatc gtcccctccc
     1141 ggtcagttca cggatcaaca ccgtgagcag ctctggtgaa ggattttcca gcgtcatgtt
     1201 accgtggcgg aactcaactt tacaggaact ggcactgact gtgctttgtg aaggagtgga
     1261 taaaagcgga gtaagagccg ccataggctc tttctgctca tcaggcgtta tctcaacagg
     1321 taataattca acgccagcgc cagaagaggt tgttaccgga agacgccgcg atatacgcct
     1381 ttcgttctgc cagagcctga gccatttgaa caggaggtta tcattgatat cgtgttcccg
     1441 ggcaatacgg gcaacagagg ctcctggttg tgaagccagt ttaaccattt gaagtttaaa
     1501 ctcatttgaa aatgatctgc ggggttctga ggataatatt ttctgttcca taacaggtgt
     1561 ccactagttg aaaaggtggg cacctacgtt accaatactg gcttaatggc tacatacggc
     1621 ggtcagttta cgcttacgag aaagaagcgg tccgccggtc gtctgttttg actcgcagac
     1681 cggacgggcc ctcagatatc ctgaggcctg gctgcagggc tggtgtggcg gtacgctggt
     1741 gagtgacgga tacagcgtgt acaaatctct ggcggataat gaccccggta ttacatctgc
     1801 ctgttgctgg agccacgcaa ggcgcggttt cgcaaatctg tataaggcca gcagggagcc
     1861 ccgtgcagcc atggcgctga gaaaaatcgc gggtctttat cgcattgaga agttcatccg
     1921 tgaacgtcct gtggaaaaaa tacggcaatg gcgacaacgg tattcccggc cgatagtgaa
     1981 tgacctgttt gcctggcctg aagagcagga gccgtgctgt ccgcccgacg gaccactaaa
     2041 caaagcgatt aattatatcc tgaaccggcg cgatgaactg agctgcttcc tgagtgacgg
     2101 agccgtgccc ctggacaaca atatctgcga gcgtgctatc cgcccggtgg tcatggggcg
     2161 gaaagcatgg ttattcgccg gttcactgat ggccgggaac cgcgcggcac agataatgag
     2221 ccttctggaa acggcaaaac gtaatggtct ggagccgcat gcctggctga cagacgttct
     2281 gacgcgtcta ccggagtggc cggaagaccg actggaagag ttgctgcccc ttgagggatt
     2341 taccttctcc gggtgagtga tacctgccat caggtgttcg tgcagcgggc cataacccgc
     2401 agtcgggagt tgaacgcctg acgacaggaa atgagccaga gcagcgacca cgcgggctga
     2461 cagtgagtca gaacctgaat cagcccggag ccattcccgg ggctgtactt catcacctgc
     2521 catcagtacc cgtgtcagta cttcgcgacc tgacgcaatt acccgactca gttcttcaga
     2581 acccgcctcc gataccagcc tggcggtcgc cccggcgcaa cagaataaat caaccatcgt
     2641 cagactgcga tcattcgtat gcatcgctga tgatgcgccc gaaaaacgcg tcaatatgag
     2701 ttcgccggaa tattactgac gacacggttt gtagttacgt tccttcggtg agtgagtacc
     2761 tgtgcgtcag ggtaacgcaa tcagttcagg tttagcgggg tgtcaagtcg gtttggctaa
     2821 ccgaccgcag aattaactgt acctttaaag gtcgcccccg atatatctgc ctgtcagaca
     2881 tatcgccgat tcggtatctc actacataaa actccttaaa aaacaatgcg attctgttgg
     2941 tacgaagctt tcagtaagtc agtccggaga agcgccagta cgatacagaa tgaggttgaa
     3001 aaaaacgttc acgtctggtc acttcttcaa atgttaaaaa acatctcgta aagagccagt
     3061 ttatgcgggt tcgttgagta gacgtgcgag cgtcactttg tcagctttaa cgctgacgaa
     3121 agtgacgccc gcacagcttc aaactccaca tgcacacgca agcgaccaaa tttcaaatgg
     3181 tcttatgccg tgcgcacagt cataagaagc gcatgcggaa agtcagaaaa tatcttaaga
     3241 cgcagatttt aaaggcgttt ttatgccagt ctgccgggaa tacacagtag tccggttgtc
     3301 cggaagcacc acggtaatcc gaaggaaaaa tcaacgtacc tcaatcaccg tagtgatatc
     3361 cgttcccaga ggctaaacgg atgtaccacc ataaaggcat cctctttcag gctacgagga
     3421 tattgcaatc acccgaaggc gtttctctca gattgctgaa acattggctg gcagtcaccc
     3481 gaaggcgtcc ctctcagact gctggaacat tggctggtcg caaaagcgac agttaatgca
     3541 aatacaggtt aacaaaaagg gaaatctgcc gtcaacccaa ccatatgaac atgttgat
//
LOCUS       DAZGJW010000282         3581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZGJW010000282 DAZGJW010000000
VERSION     DAZGJW010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.285"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(51..656)
                     /locus_tag="ACHKIK_005048"
     CDS             complement(51..656)
                     /locus_tag="ACHKIK_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFO9086024.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAAMQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
     gene            complement(913..1065)
                     /gene="kil"
                     /locus_tag="ACHKIK_005049"
     CDS             complement(913..1065)
                     /gene="kil"
                     /locus_tag="ACHKIK_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO9086025.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PCYRRK"
     gene            complement(1050..1181)
                     /locus_tag="ACHKIK_005050"
     CDS             complement(1050..1181)
                     /locus_tag="ACHKIK_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO9086026.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVEVLLCAMK"
     gene            complement(1206..2174)
                     /locus_tag="ACHKIK_005051"
                     /pseudo
     CDS             complement(1206..2174)
                     /locus_tag="ACHKIK_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
     gene            2430..2969
                     /locus_tag="ACHKIK_005052"
     CDS             2430..2969
                     /locus_tag="ACHKIK_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="super-infection exclusion protein B"
                     /protein_id="HFO9086027.1"
                     /translation="MSNWWQELLRFFLRGLTLQQLIHMLIILIALIIITPASIKEWVD
                     IRNPEILPDHWMYYAMLLCISYVLNRGMEFIFLASSDRYKKYLSKRDEAKVIVETERL
                     FNSLSIQEKEVLAFAVMANNKIVLKHGDPVALSLMRKGLLHRSGVTYSASGKEKFVIP
                     DVWFHECYMRFAGKADELI"
     gene            complement(2993..3265)
                     /locus_tag="ACHKIK_005053"
     CDS             complement(2993..3265)
                     /locus_tag="ACHKIK_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFO9086028.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQEAISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVAIYAAGYRKSKQLTAR"
     gene            complement(3243..3462)
                     /locus_tag="ACHKIK_005054"
                     /pseudo
     CDS             complement(3243..3462)
                     /locus_tag="ACHKIK_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          782 a          827 c          762 g         1210 t
ORIGIN      
        1 ctgaaactct cttgcgggct tgtttagtct tacagtaatc gaatgtgcca ttaagcagca
       61 gctccttcta attcgtctcg tctgatgttg taaacgtcct gcgctttgtg ctgctccggt
      121 gtgccttcga gcatcttcca cgctttggcg aacgcctgtt taagctcttc tacggtgttt
      181 ttctgcattg ctgcgtcagt gaatgctttt agaacctgtt caggtgtagg tgatggtttt
      241 gattgctttg ctgctgcgtt ctgctgatgt ttatgctcgt cggtatctgc atctttcgca
      301 tcatcaatgc cgaataaacc attgaggcaa tacttgcgtg cataagagct tgtagctccc
      361 gtaacttgtg cagaatccat tcctttcttg ctttcttcct ctcgtgcaag agcggttgcc
      421 gtatgactgt tttcgccatc ggtaatagtt gccgtggctt tcacataata ccgatcacca
      481 atcaacacaa cttcatcgct gattgataaa aacaggccat tcagtaacgg cttaacgcct
      541 tcaagaatat cttcgcagct tctgtattta tatttgccga atgagttgta ctgattcttt
      601 ggcgcgttca gattctcctg aatagctgcc agtcttgcgt aaaattcttt gctcatatga
      661 ttgttctcag aatggacacg gcccaaggaa ataacgctga tttaatactt cgactcggga
      721 caaattaagg catacccgca ttccttcgcg gtcaccatta tggcgatacc agagagcttt
      781 ctgcgtgtac atgcgcctct gtaacttgct ctccttcact gtggttgcaa gtgacatgaa
      841 tatctccttc gttaccgatt aattctttca tctgacgaat gaattcttcg tctgaccagt
      901 tatctgtaaa actcatttcc tgcgataaca cggaaggttg atagctgatt tcatcgcttt
      961 atttgcttca agccacattt ttgaatcacc aataaatcgg gctattactg ccttgttctg
     1021 tgcagcacga agcatctggt gattgatggc tatttcattg cgcataataa gacctcaact
     1081 cttttccatc cgtcacgtaa tttacgggtg attcgttcaa gtaaagattc atttaattgg
     1141 aaggcaccca tgcgagcgcc tcccgcgatt gcgtaaatca tgggtggttc cttatgttgg
     1201 ttttattagt aggttatttt tgttgcgaat aattcgcctt ttacgatggc tgttatgata
     1261 tttttagcaa catcttctga tgcgccaacc ttgataaggt cagcaagtat tttgttattt
     1321 acttctttcc ggtgagcttt atcctttgct ctacgctctt cttcgtcctt gattcttttt
     1381 tcttctgcta ttctggcttg ctcttttgct tcagcctcgc gcaggattcg ttctgcctcc
     1441 tcctgtgctt tacggcgttc tgcttcaatt gccgcatgct tttctctttc agctcgttct
     1501 gctgcctctt ttgcttcgcg ctgtgctcgt tgctcggctt caatgcgttc acgctctgca
     1561 cgttcctctg cggccttagc ttctgcttct cgccttgctg ctgcttcaat ttcggctttt
     1621 gcctttgctt cggcttcagc tctggctttc tcttcagctt ctctttttaa gcgttcttca
     1681 tgctctcgct tttcctgctc cgctttgagt cttgcctctt ctctttggcg gtcaaattcg
     1741 cgatccatca aaatcgctat ttcatggtca gactcaattt gctttgcgag agcttcagct
     1801 gctgccttag cttcttcttc ggctttaatc cgcgcctgtt cttcctcata atcagtaaga
     1861 ggctggcgcg ccttggcttt cagttcatca aggcgatcgc gcactgtctt gcggttagca
     1921 tcaattagct ttggaatttc cttcagttca gcaacaaggt ctttgccaag accatcgaga
     1981 tatgttttcg tctgcgcaac tttatacgcc agataagcga tctcctttct gccctttgcc
     2041 gttgtgatat caggcacaaa ggacataact tcacgttcaa ccttttgaag aatttcttca
     2101 atctggtcgg cagactaaat tacagtcatt gcatttgctt tttcaataac aactaaatct
     2161 gttacttcac tcatatatcc tccgtcaaaa aaattgccct cacactggag ggcaaagaag
     2221 atttccaata atcagaacaa gtcggctcct gtttagttac gagcgacatt gctccgtgta
     2281 ttcactcgtt ggaatgaata cacagtgcag tgtttattcg tatgcctgtc ttttaaccac
     2341 atcaggctcg gtggttctcg tgtaccccta cagcgagaaa tcggataaac tctattcacc
     2401 cctacagaga gaatgatgga gattcaccga tgagtaactg gtggcaggaa ctattacgtt
     2461 tcttcctgcg tggtcttacg ctacaacagt taattcatat gcttattatt ttaattgcct
     2521 tgataataat caccccagca tcaattaaag agtgggtaga tataaggaac cccgaaatac
     2581 ttccagatca ctggatgtat tacgcaatgc ttttgtgtat cagttatgtt ctgaacaggg
     2641 ggatggagtt tatatttctg gcatcttcag acagatataa aaaatatctc agtaagagag
     2701 atgaggctaa agtaattgtg gagactgagc gcctattcaa ttctctgagt attcaggaaa
     2761 aagaggtttt agcatttgct gttatggcaa ataacaaaat cgtacttaag cacggcgatc
     2821 cggtcgcttt atctcttatg agaaaaggcc ttctccatcg ctcaggtgtg acttacagcg
     2881 cgtcaggtaa agagaaattt gttatacctg acgtctggtt ccatgagtgt tatatgcgct
     2941 ttgctggtaa agctgatgag ctaatttagt tcctctacgg cgggggggat cgtcacctcg
     3001 ctgtcagttg ttttgatttc cggtagcctg ccgcgtaaat ggctacgttt ggaagacaag
     3061 ttgaaccttc atattttctg gtcaacgttg tcagagttat aacttctgct ctcattgctg
     3121 gtttgcgctt gcattgcaag accactcgtg aaggggttgg cctgtgtagc ttgtcggagc
     3181 taatcgcctc ctgactttgc aggtttgcgc gacgagctct acggcgagaa gctgcggtgc
     3241 ctttaaattc tgtttttctg gacatagatt cctcccgaat aaactttggc gatgcaatct
     3301 cgaagctcct cctgagacgg ttacttcggc attgcatccc acagcttatg tggttgggtg
     3361 atctggcttt tcagccacgt agtcgagtgt tcgacgttgt ttaaagagcc tgccagtctg
     3421 ctccgtttgg cttacagcgt cctgctgatg agttgacttt atcgaaatga taaactgatg
     3481 gcaatagcaa aatgataaat tgtttgggtt ttcaaatatc ctgttgatta ttatggtttt
     3541 ttatttgatt gcgggaattt atcagaagga atgaagagac t
//
LOCUS       DAZGJW010000283         3572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZGJW010000283 DAZGJW010000000
VERSION     DAZGJW010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.286"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(112..451)
                     /locus_tag="ACHKIK_005055"
                     /pseudo
     CDS             complement(112..451)
                     /locus_tag="ACHKIK_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(767..1411)
                     /locus_tag="ACHKIK_005056"
     CDS             complement(767..1411)
                     /locus_tag="ACHKIK_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086029.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            complement(1432..1701)
                     /locus_tag="ACHKIK_005057"
     CDS             complement(1432..1701)
                     /locus_tag="ACHKIK_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO9086030.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            complement(1780..2418)
                     /locus_tag="ACHKIK_005058"
     CDS             complement(1780..2418)
                     /locus_tag="ACHKIK_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFO9086031.1"
                     /translation="MGDSVTPEILGNMIRQYFSQERPEEEAIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            complement(2403..3572)
                     /locus_tag="ACHKIK_005059"
                     /pseudo
     CDS             complement(2403..3572)
                     /locus_tag="ACHKIK_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT          901 a          987 c          757 g          927 t
ORIGIN      
        1 catactcctc cggcagaagc agaaaaagct tattatgctt ccatcggaaa cgatgatctg
       61 gcagcctgag ttcacagata aaacactctc caggaaaccc ggggcggttc aaacctgatt
      121 ctggaaaagg actttgtgga ttgttgctat tgccagtgag attgaatgaa gtcgttttaa
      181 ctcgctttga atacgtcttg cccccagatt tcgctgagaa cgtaactcca gaatcagagc
      241 gacttcgcaa gtgccggttt tcgttgatgg tgaacgcttg gggcgacggc tgtggctttc
      301 cagaccagcg atgccctgag ccagatatct gcgccaccat tttacgcaat gtgggccgag
      361 aaatgccaca gcgacggcat acaaatcccg catcaccgga cgtttcatag agttttaccc
      421 actgtagccg ctgctgaatt tccctgttca taatttggca ttagttcagc tcaatcccga
      481 cttaaaaagt ccctacagga tgttccggac ctgaggcaca gttcatatcc ttatccataa
      541 aaaatttagc tatgtctata gcattaatct tgacaagacg acgttagggc ctggcgatga
      601 atcatttccc ctgccagata tcctttgggg tgcaccagaa ctaccgtttt atatctatat
      661 aactgaaacg tccctcgcca tccttcatat tttgatgtag taacaccatt cactttcatc
      721 taccccccgt tttattaaca tatagtaaaa atcacctcgc cgcattttat tcttttgaag
      781 taaaatcatt taccagttca cttaaagctc tcttttgttc atcatcatac agagattgaa
      841 tccggaattt aatctcttct gcaaccagtc ttgcatcggg ctcataaaat atttttgtta
      901 ttcgctgttt ctgatgttca ggctggtctt ccatatttcg taaaaccaga tgaaataata
      961 tatcataaag tgcgcaatgc catgaataac catcattggt taaaataagc tcccaaaccc
     1021 gctcctgctc ggcaataagc tgcagaacaa aggactcaac ctgttctgtt tgttcggggg
     1081 caacacggta tagcggagca atcacagcaa aagatagttc atttaaccag gattccgctt
     1141 ccttctcgct cctgaacggt ggacagtttt gtattcttcg ttcgtattca cgggtaatct
     1201 gctccagaat atcctccggc ggtgtcagcc acttattcct gagcaataca ctggttgatg
     1261 gatgacagct ggctatttca gcaataattt cacgtaatgt ctccgggctg aacttcttca
     1321 gctgattgta gtgctcccgg gtaaaaccat gttcatcgca taaaagttgc catgtcattg
     1381 atatactgtt tgccattcct tgcttcatca ttaatacctg ttacctgaat atcaacagac
     1441 cggtgtacct tttctgaata cctccccgga cggagtggca atccccattc gcctcagcgt
     1501 ggcatctgca gctttcgcca cctcgctgat atccagcgtt tcaggtagcg gcggggacga
     1561 tatctgcgcc agacaataat tccggcgcaa caacaaccac tcccgttgtg cttctgtaca
     1621 ttcaacattt cccataacaa aaagccagcg gtcagaagct ctgcgccagc aaccagcagc
     1681 ctccagtttc tgcgcaacca tgtcttccac aacttccggg gtactctttt tcctgctcaa
     1741 aacattaact cactgaaata atatacatat ctgacggtct cagcgaaccg tccatgccgg
     1801 gctgaaatcc tcttcctgaa acagctccgt cagcccgcta atctgtttca atctgagaac
     1861 ttcatcctgg tccataccca gctccgtacc aatgcgttca ttcgtccagc ccaggcgcga
     1921 caggtccctg acaatatcag acattgaggt tatctgatgt ttgcccctgg cccggttatg
     1981 tcggatggtc gcagcaatac gtgaggcctg tccctttctc tccggattaa tacagaccac
     2041 cggcagccag cccttcagac gttttcccgt acctgcttcc ctgcccagta cctggcgatg
     2101 aaaaccatcc accaccaagt aatggttctt ttcctcagac actaccaccg gttgtgtgaa
     2161 accatctttc tccagtgact gcttcagcag tttcttctca cccggcgcca tgacattagg
     2221 attgtaatca ttggccacca cctcatctgc ttttacccac agcacacagt ccacaggctc
     2281 ctgtgcaaaa ggactgacct catgaagcac cctgcgcagg tgattaaggg cctgtatggc
     2341 ctcttcttct ggtcgttcct ggctgaagta ctgacgaatc atgttgccaa gtatctccgg
     2401 tgtcacagaa tcccccattc cttcctcctt tccttcatac gctgcaggta acgttcatag
     2461 tgccggggtt tgttagggct aaaggagagg gtccggcacc agaaatcatt ctttatgaga
     2521 gtcttacaga tacgtcgcca ggacggaata tcccggctcc ccaggtcatt ctcctgttca
     2581 tccggaatgt catccgggaa gccccgcgtc tgataccaac gcaggtagac agcaatttta
     2641 ttacggtaat gttctttccg gcatcacatc cagcaggaac atcgcatagc tgcgccaggt
     2701 atgatgaggc ggcttcgtga tacgcttaca cagggcaaaa taggcaccac tctcattggc
     2761 ataaagcgcc ccgctggcag cccctgacac ccgttcacac atcctggccc aggtttccgg
     2821 ctccagaaca tggtaaagcc acagtccctt acgctgttca ggaccaaaag gttcacagac
     2881 acgcatgttg cgtaacggca cgccggcacg gtacatcagg tcatacagag gattgtaaat
     2941 ggcacaggtt ctggcgttat atatccagat atcgcgggcc ttccagtcat acaacggata
     3001 catggtgtaa taaaaccctt caggcgatgc cgtggtccag ggcttatcat cggcatatct
     3061 cagtttgcgc tgagatacca gtcccataaa gcgattgagc gattcatcag cacggacacc
     3121 agtcagtatt gccactccac accggttacc ggcaaaccag gaagaaaatg ccggaacaaa
     3181 ttcttcaaac gtcatggcat accggtaaaa gggaaaatac gtcatgtctg taatggcctc
     3241 ttccggtggc tggcgaaccc aggtcactcc tggctcccag catatccact ccggctgaaa
     3301 ctgcgagacg ccgtttaccg tggtcaggga agtgccaccc agtaaaaggt ttccgtcaca
     3361 tcatggtaca tttcccgcat cttctgaata tgttcaatag tgcaccgata ctgggcctcc
     3421 cagtcaatga acagaacaga gaaatgacgt tttctcctgc gggccacctc tgccacaaga
     3481 tggaaaagca cagtggaatc ctttccacct gaaaaagaca ggcataccga cggaaatgtt
     3541 tcaaaaatcc actcaatgcg ggcgattgcg gc
//
LOCUS       DAZGJW010000284         3563 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZGJW010000284 DAZGJW010000000
VERSION     DAZGJW010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.287"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(242..877)
                     /gene="nth"
                     /locus_tag="ACHKIK_005060"
     CDS             complement(242..877)
                     /gene="nth"
                     /locus_tag="ACHKIK_005060"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO9086032.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     LVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(877..1572)
                     /gene="rsxE"
                     /locus_tag="ACHKIK_005061"
     CDS             complement(877..1572)
                     /gene="rsxE"
                     /locus_tag="ACHKIK_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO9086033.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(1576..2196)
                     /gene="rsxG"
                     /locus_tag="ACHKIK_005062"
     CDS             complement(1576..2196)
                     /gene="rsxG"
                     /locus_tag="ACHKIK_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO9086034.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(2200..3258)
                     /gene="rsxD"
                     /locus_tag="ACHKIK_005063"
     CDS             complement(2200..3258)
                     /gene="rsxD"
                     /locus_tag="ACHKIK_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO9086035.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(3259..>3563)
                     /locus_tag="ACHKIK_005064"
     CDS             complement(3259..>3563)
                     /locus_tag="ACHKIK_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086036.1"
                     /translation="NAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRK
                     AAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT          877 a          928 c          896 g          862 t
ORIGIN      
        1 aaatttaaat gattttaacc tgatttttgc aatgttttta cattgccaca gcatcgtgat
       61 cttgatcacg aatgaataga aaatttcgct attaaaaaaa caattaacct gcatttttgg
      121 ttatccgcaa acggattacc tgatgataac gctaagcaat ggcatcattc acgttaaaaa
      181 gatgttttaa aagaggataa agaaaggtta tcaatggggt aatcggtgtt accccttttc
      241 ttcagatgtc aactttctct ttgtattcac aaagatcttc aataatacaa gagccacagc
      301 ggggcttgcg ggcaatgcag gtataacgcc cgtgcaggat caaccaatgg tggcagtcga
      361 ctttaaactc tgccggaacc acttttagta gcttttcttc tacctgttcg acatttttcc
      421 ccggcgcaaa ttgagtacga ttacaaacgc ggaaaatgtg cgtgtcgaca gcaatagtcg
      481 gccagccgaa tgcagtgttt aatacgacgt tggctgtttt acgacctacg ccgggcaggg
      541 cttcaagcgc agcacgatct tccggaacca agccattatg ctgctccagt aagatacggc
      601 aggttttgat gatattttct gctttgctgt tataaagccc aatcgttttg atataggttt
      661 taaccccttc aacgcccagt tcaagcatcg ctgcaggcgt attcgccacc gggtagagtt
      721 tcgccgtcgc cttattaaca ctgacatcgg tcgcctgagc ggaaagcagt acggcaatca
      781 gcaattcaaa aggcgaactg aaattaagct cggtggtggg atgaggattg ttctcacgca
      841 ggcgagtgag gatctccagg cgttttgctt tgttcatcag acattccctg tttcaccgtt
      901 tggcaatgca cgttcggcag ctgcttcagt acggcgcttt ttcattcttt catcaatcag
      961 gtattttcct gccagcatca gtcccaggcc aataaatgca cctggaggca gcatcgccag
     1021 caggaaaggg gagtcggtgt ggaaaatttc cacgcgtaat acttttgccc agctacctaa
     1081 cagcgcatct gcaccgtcaa acaatgtgcc attgccgata atttcgcgta gtgaacccag
     1141 cacgaacatg gcgcaggttg cgcccatacc aattgaaaag ccgtccagtg ccgaaagcgc
     1201 cggacctttt ttggcggcga aggcttcagc gcggcccaca acgatacagt tagtgacaat
     1261 cagcggaata aaaatcccta atgattgata caggccaaag gcgtaggcgt tgatcagcat
     1321 ctgtacagcg ctgaccaccg aggcgatgat catcacgtaa atgggaatgc ggatctcggc
     1381 tggcgtccag tgacgcagcg tcgaaatggt caggttggtc agcgtcagta ccagcgtagt
     1441 cgcaagtcct aaacccagag cgttagtggc agtggacgtg accgccaaca gaggacaaag
     1501 gccgagcaac tggaccagcg cagagttgtt tttccacaac ccctgaacaa taacgtcttt
     1561 aatttcgctc acgctttatt ctccacaggc aggaagttga gaaagttgtg ccggtaacgt
     1621 ctgagcgtac aatccggcgc gttttaccgc attaaccacc gcgcggggag taatcgtcgc
     1681 gccggtgaac tggtcgaaat caccaccatc tttcttcacc gcccagtgcg catcatctgc
     1741 accactgatt tttttacccg caaaatgggt gatccagtca gaaaggcgca gttcgatttt
     1801 atcgccaagc cctggcgttt cgtggtgctc tgtcacgcgc gtgccaagta ccgtgccgtt
     1861 aaaatcggct cccaccagca gctgaatcgc accggaatag ccatctggcg cggttgcttc
     1921 cagaacggcg gctaccggtt tgtcatcctg tttggcgatg taaacccgat gctcaccttt
     1981 acctaactct ggcgcagtta ccagatagca actctgtgcc agcgcattgt tatagcgttc
     2041 ggctggcagc acctgatcaa ataatgcctt ttgttgcaga ctggcctgtt cagcaatcgt
     2101 cgttttggtc atctggttga tggccgcagt taaccctgtt gaacccgctg caaatagcgc
     2161 taacgtaatg ccgtgttttc ggatagtttt cagcatggtt taccctttgc gatggccgta
     2221 gacgcgcgga cgcgtgtagt aatcgatcag aggaaccgtg atgttcgcca gcaggacggc
     2281 aaaagccacg ccgtcaggat agccgccgaa actgcggatc aaccagacta ataagcccgc
     2341 cagcgcgccg aaaataagac gaccacgatt ggtcgtagaa gcggtaaccg ggtcagtcaa
     2401 aataaagaat gcgccgagca tggtcgcacc agacagcaga tgaatttgcg gtgctgccag
     2461 tgtttctggt gagaacaacc agcccaacgt tgcgcataac gccagcgtta ctaagaagct
     2521 gaggggaata tgccagcgaa tcgctttctg ccatagcaac cacaggccgc cagccagcca
     2581 ggcgagattt acccattgcc aaccagcgcc cgccagaata ccgctgtaga tcggatattg
     2641 cataatctgt tcaaccgaat gaccggcacg gacagaggtt ttaaatgtat ccagcggtgt
     2701 cgcctgacta atgccatcaa tacctaagcg tagtgtgttc atatcaccac cactggcggt
     2761 atgtccgctg aaaataacct gtatggcgtc gataaaacca gggatgttga ccgcaatttc
     2821 atgtggcggt aaccagctgg tcatctgcac cgggaaggag atcagtaaga ccacataacc
     2881 aatcattgcc ggattaaacg ggttttgccc cagaccgcca tacaactgtt tagcgatgat
     2941 caccgcaaac accgtaccca gcacgaccat ccaccatggc gcgagggggg gaatacttac
     3001 cgccagcaat aagcctgtca gcaatgctga gttatctttc aacgttgcgg ctaccgactg
     3061 cttgcgtagt ttgagtacga gagcttcggc taacagagca ctaaccgatg ccaacaggat
     3121 ctgaacgaga gtaccccaac caaaaaacca cagttgcgct gcgattcctg gcacggctgc
     3181 gagcaacacc aacagcataa tgcgcgatgt ctggcgctgg ttatgggtat aaggggagct
     3241 agctattctg aataccattt agtcctcgtt tacaaccttc tgctgggcgg cttttttggc
     3301 ctgagcgcgg gcaatagccg cggcaactgc cgctttgcgc ggatcaacct gttcttctgg
     3361 ttctgcgtta gtctgttgct gttccagctt gcgcgctttg gcacgggcaa tagcggcttc
     3421 gacggcggct ttgcgcggat caacctgttc ttctggttcc gcattagcct gttgctgttc
     3481 cagtttgcgc gctttggctc gggcaatagc ggcttcgacg gcggctttgc gcggatcaac
     3541 ctgttcttct ggttccgcat tag
//
LOCUS       DAZGJW010000285         3543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZGJW010000285 DAZGJW010000000
VERSION     DAZGJW010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.288"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(51..248)
                     /locus_tag="ACHKIK_005065"
     CDS             complement(51..248)
                     /locus_tag="ACHKIK_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO9086037.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            complement(480..1022)
                     /locus_tag="ACHKIK_005066"
     CDS             complement(480..1022)
                     /locus_tag="ACHKIK_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO9086038.1"
                     /translation="MIYPEITGKSGEHLRLKTLEAVWIQGKLRMWGRWSYIGGGKPGN
                     MFNQLLASKKLTKTAINEALRRIRESGIDKPELEAFLREMIAGRQKSWLSHCTDAEAL
                     RIDGVISKALARYPGLIDILRQRYEGRGMSKRKMAELLNEVHPEWCFSTCEKRIANWL
                     AVAEYALYIPMRESFAQKMS"
     gene            complement(1031..1396)
                     /locus_tag="ACHKIK_005067"
     CDS             complement(1031..1396)
                     /locus_tag="ACHKIK_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9086039.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQRVPGGRLGVKIYKIESE"
     gene            complement(1397..2452)
                     /locus_tag="ACHKIK_005068"
     CDS             complement(1397..2452)
                     /locus_tag="ACHKIK_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9086040.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRNQTSESLGQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWATINNVADALPETVLRRSLGLRAEKIRSVYRESDIVPGEQTATSMLKQR
                     TKNIALLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPRREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            complement(2454..2726)
                     /locus_tag="ACHKIK_005069"
     CDS             complement(2454..2726)
                     /locus_tag="ACHKIK_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086041.1"
                     /translation="MVHKTKFHNSDTDLAIRHGRRSHAFKTDWYQHPPCTEEQAEWLI
                     HNYRRRGYEIKKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            2673..2852
                     /locus_tag="ACHKIK_005070"
     CDS             2673..2852
                     /locus_tag="ACHKIK_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086042.1"
                     /translation="MPDGKVGIRIVKFGFVHHLFSLLAQQVSGVQADVRIVNQNARKK
                     QNPPKRVKCGCVEDA"
     gene            complement(2894..3049)
                     /gene="hokD"
                     /locus_tag="ACHKIK_005071"
     CDS             complement(2894..3049)
                     /gene="hokD"
                     /locus_tag="ACHKIK_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO9086043.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          845 a          942 c          808 g          948 t
ORIGIN      
        1 aggtgccgct aacacccacc agccgcccat taccacaaat taaaaagcct tcactgcgga
       61 aggcgtctgt aacaaccgaa ctgataatct gccagacccg ccataacaag ctgagtcagt
      121 attaactggc agcgttcgcg tgaaaggtaa gtattctgcg caatttcccc gacggtcgcc
      181 ggttcggtga cgcttaattc attaaacacc actctggcgg tttcggtcat atcctgctgt
      241 tttagcatgc ttttttccct tttccggtta acgtgacata ccaatacctc ttgtcgaaaa
      301 agccagcaag ctgaaagacc agtattcaca actaccagcg cgtttaatgt tctgtgccgt
      361 ttttcagaca taaaaaaacc cgcataaagc gggttctttc aggtgtccat gtctgctatt
      421 cgcctcgcgg tatagctttg cgaagcgtag ctggattgaa acagtttatg cgtaaaaaat
      481 caagacattt tttgagcaaa cgattctcgc atagggatgt atagcgcata ttcagcaaca
      541 gccaaccaat tagcaattcg cttttcgcat gtactaaaac accattccgg gtgcacctca
      601 ttcaacaatt cagccatttt gcgtttactc atcccccgcc cttcgtacct ttgccgcagg
      661 atatcaatca atccaggata acgtgcaagc gctttactta tcaccccatc aatgcgtaac
      721 gcctctgcat cagtacagtg agacaaccag ctcttctgtc tgccagcgat catctctcgc
      781 aagaatgctt ccagctctgg tttatcaatc cctgactccc tgattctacg cagggcttca
      841 ttgattgcgg tttttgtcag ttttttggat gccagcaact gattgaacat atttcctggt
      901 ttgccaccac ctatatacga ccaacgcccc cacatccgta atttcccctg gatccagacg
      961 gcttccagcg tttttagacg taaatgctcg ccgcttttgc ctgtaatttc cgggtatatc
     1021 atatttacga tcactcactc tcaattttgt aaatcttcac gcccagccgt cccccaggaa
     1081 cgcgctgacc gcgcacaata ttgatttcat caaactgctc gtcgtctatg agaagtccgg
     1141 catgcgtcag cgcatccagt ggtgctttca ggatattgtc caggtcacga cgacgtttat
     1201 ccggtggctc tgcaataatc tttatcgcca gccttccgga caggtttaat ttcagccgct
     1261 gctggcgaac aataagcgcc acatcacggc gataacgctc accggctttt gatacaaaat
     1321 atgtgctgcc acgacgtcgc cagtaagtgt tcaccgttgg cgggtaaggc aaaacaaatt
     1381 ctatgcgttc agtcattcat gctttccact tcaggacacc cgaatttctc gcgtgcatta
     1441 aaaaacgaat cagcaacaac agctggctgc cgtgtttttc ttcaaaatct tttaccccgg
     1501 cgtgtagttc gctatggcat ttacggcaca gcggaataac aaacaaatca tcagcctttg
     1561 ttcccatccc tcccagtcca tgaccaatga tgtgatgcgg atcatctgcc tgattaccac
     1621 acgtcatgca tttctgcgtt tttacccaac gcgtgtatac aggcatctct tcccgtcgtg
     1681 gtttctggcg ctggagatac tgagccggtg actccggatc aacggcaatg cttaccaccg
     1741 tcttttcctg tggcgggttt tgctggtggg cgtgaggcag tagcgcaata ttttttgtgc
     1801 gctgcttcag catgctggtg gcggtctgct ctcccggcac gatgtcgctc tcgcggtata
     1861 ctgagcgaat tttttccgcg cgtaatccca gtgaacgacg taacaccgtc tccggtagtg
     1921 cgtccgctac gttatttatg gttgcccacc aggataattc agccagcgat aattcccgct
     1981 cctgcgtgcc attcattgcg tggcggatga catcaatcat ccatgctgac aggttttggt
     2041 gagcaagctg cccgagtgat tcggaagtct ggttacgcag ctggttgtcg cagtgccagc
     2101 acaacaccat cgcgccggta ccgtaacgat gtatgacggt ttcactgtga tggtagtcac
     2161 catgaggcca ctggcaggat ttaatgtggc gtaacagcca gtcagacaat gcaccagcac
     2221 caccagcagc acgaatcacc cgctcatcgc tgaaaaatgg cagtaatgat ttatcctccg
     2281 ccagcggctg gcgaacagca ggaacgactc cggacggcag accgcgcatg cttttcggtt
     2341 ccggctccac cagaactcga gggttatgaa atacctgcat ggattcacgg cccggtttta
     2401 gcaccaccag cccaagttcc ggtaccggaa caggtcgaag taatacccgc acgttacctc
     2461 cagatgcgtt gctggaatgt gcgggacgga cgcggtgggc gttcggaata agggagcctg
     2521 acatagatta tccagtgacg ataatcgagg ctgagggctt tcttaatctc gtatccgcgt
     2581 ctgcggtagt tatgaattag ccattcggcc tgttcttcag tacatggtgg gtgttggtac
     2641 cagtcggttt taaatgcgtg tgaacgccgc ccatgccgga tggcaaggtc ggtatcagaa
     2701 ttgtgaaatt tggttttgtg caccatctgt tttctctgct ggcgcagcag gtgtcaggtg
     2761 ttcaggctga cgtgcgaatt gtaaaccaga atgccaggaa aaaacaaaac ccgccgaagc
     2821 gggttaagtg cgggtgcgtt gaggatgcct gactcatcag aggtggcgag ggatttctcc
     2881 ctcgcctggt ctcttactcc tcaggttcgt aagctgtgaa gacagcgacc tccgtctggc
     2941 cggttcggat tcgtacctcg cagaggtctt tcctcgttac cagtgccgtc actatgacgg
     3001 ttaaacagat gacgatcagg gcgattaaca tcgccttttg ctgcttcata gcctgcttct
     3061 ccttgccttt cggcacgtaa gaggctaacc tacatgtgca aagcatgaaa ttggcctcag
     3121 attaatgtta agcgtcttgc cggacgcgta atgttaactg gggcttttct ctatctgcct
     3181 tttggtgttc atgcctgagg cagatagcct caagcacccg cagcaattct aactatccaa
     3241 tagatcaatg ccaacttctt ttcctgcaac atctctcatc agaagtgacc caaattcatc
     3301 caatcagcac aaaagaaaca tttaaagcgt gaatgctacc gaacgttcat ttcaattaca
     3361 tcgttttgat ttttaacagt tttcacctca acaaatcaaa tggcattagc attcatcatg
     3421 gaagaaagac tgataaaaat gatcattttc aatgacttat gatcacaccg ggtattgcca
     3481 agtcattcct tttacgccag gatacccaca agtgagtagt cgaggagagt ttgccaattt
     3541 ttg
//
LOCUS       DAZGJW010000286         3501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZGJW010000286 DAZGJW010000000
VERSION     DAZGJW010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.289"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..664)
                     /locus_tag="ACHKIK_005072"
     CDS             complement(<1..664)
                     /locus_tag="ACHKIK_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HFO9086044.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFS"
     gene            complement(768..917)
                     /locus_tag="ACHKIK_005073"
     CDS             complement(768..917)
                     /locus_tag="ACHKIK_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086045.1"
                     /translation="MIKIKSILAGLLLSILVPVDAGKYRKGGSVPSQNMVGGIVRRLV
                     IVGMT"
     gene            1339..1530
                     /locus_tag="ACHKIK_005074"
     CDS             1339..1530
                     /locus_tag="ACHKIK_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9086046.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(1583..1816)
                     /locus_tag="ACHKIK_005075"
     CDS             complement(1583..1816)
                     /locus_tag="ACHKIK_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086047.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(1912..2535)
                     /locus_tag="ACHKIK_005076"
     CDS             complement(1912..2535)
                     /locus_tag="ACHKIK_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9086048.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(2624..3133)
                     /locus_tag="ACHKIK_005077"
     CDS             complement(2624..3133)
                     /locus_tag="ACHKIK_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086049.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
BASE COUNT         1023 a          887 c          737 g          854 t
ORIGIN      
        1 tgctgaactg cccggcagtc cgggcggaaa gcgtatcggc actcagttgc gcattctgtg
       61 tagacagatt tccactggtt gcattcagct cagtatgttg tccctgtatc cggctgttac
      121 ctaaatccag cccctgctca atgtcccgta aatccagcag agaaccttcg tgtgccggga
      181 atgaggaata caactgaata tagtcatcac tgtccggtgt cagacgcaca tgtcgcacaa
      241 caccgggaat aataaccagc cggaggatac cgctttttaa atcctgcgaa ggtgccagaa
      301 cacgggtggt gacataacca tgatcgacca gacggttctg caatgtactc atcagcagat
      361 taattccttt cgcccccagg caatgcccga ccgccccgtt ggcgattttt tgtaaaggca
      421 gccagtgtgg taacgcatca gcccccttca gttccgtctg tttaatctga aaacagggcg
      481 tttcaacagg aaaattaatc ttatgggcaa aagagccagg tgcagaaagg cgaacatcag
      541 gggctgaagg cgtcagttgt tgctcaaggg cttcctgacg ctgctgctga atgataaatg
      601 cttcatctcc gctacgccgg acatccgctg cctgaagaga taaaggcgat aataaagcag
      661 acatcagtgt cagtgacagc aaggacggac agcgccggaa taagctttgt tcctcagtcc
      721 tctcgttcct gcaacataat tttccagtca aacgaaaaac cttttattca tgtcatacca
      781 acaatcacca gcctcctgac gatacctccc accatgtttt gcgatggcac gctaccgccc
      841 ttccggtatt tacccgcatc aaccggtacc agaatgctga gtaacaaacc agctagaatt
      901 gacttaattt tgatcattat agaataatga tctgaactac agcaacatga cagatttatc
      961 gatcgatata acacaaatga acagttcttt attctgacaa atatcgcaaa aacagcacat
     1021 tacaaaattt ataaccataa ttactggaaa agtgcagaaa attattccac tgaatgcttt
     1081 taatacagtt ggttagctta aaaaatagca atttttgatt tcagtcattg aaagttgcaa
     1141 tgtttgacga ttcaacagca tttaagtgag tttcaggata tgggaaggac atgaaaggag
     1201 gagcgcaata aaatacacag gaagaagggg aaaatgtttc gtaaaaacag aaaattaaca
     1261 gcgcacatca ggaaacgcag tctcctgatt cagtgctacc acatgaaaat ttagttaact
     1321 gttaccgtac taaaatttat gtcagaagat gttccattac cgaaagtcaa tcagcgatac
     1381 aaagacgatc atggtgcgct tgtcaccctg accagtgttg aggaaacccg ggtggtgttt
     1441 atgcaggacg gctatccgca tccctgcatg cgaccgatgt acaactttct gggaaaattc
     1501 aaacccgaac cccgggaaga aacagaataa aacgaaatcc ctgccggcaa tcacactacc
     1561 tgatagcagc ataatccact tattaattct catccccgga gacaagccca gaaaaccact
     1621 ccgggtgttc actgtatgcc gtgttcagcg catccctgga aacgtttata taaaagctgt
     1681 cctgcaattt gcgggtaaca acttctgcca ggcgattccg gttatccacc agtccggcat
     1741 aacctctctc tgccagccac tcattcagtt catgttccag agacagattg ataatatcat
     1801 aatcggacct tgccattccc gtttccttag cacatgtttt caggcacggg gataaagtat
     1861 aaagaatgtc atactgctgc tgccagagca atattccccc tgatggttgt atcagagatg
     1921 gtatgccggt actttgcggg ttgttcccgt caggttatcg tgcacctcca cggtcgtggt
     1981 caccaccggc attccggcct ttctcagcct cagaacatca gccgcaatac gctgactgcc
     2041 aaaccagaac aggccgtcca gtgcagtcac cagtaaaccc gcctccagtg catacttcag
     2101 ccgttcacgg ggcgctttca cttcccgggc aacctgctga tatggcaatg atgtgttttc
     2161 attcccaacc acgcgacgaa tacggtgaga cagatgatac cggtatgtat ccggcacacc
     2221 agaaaggctg gccttcaggc tgtacacgca gccaaaccgt ttatcgttaa acaccacatt
     2281 tttctggctg atgccccatt cttcacgcag cgcggcaatc aattgcggtg tacgggtaag
     2341 cagcaagcga aaaggcagtt caaaactggt gacgtaatcc acattcagca gtgcaatacg
     2401 caaccgttct tctggcccgg cctctgtctg ccggcactcc tccagaacat cctgccactg
     2461 ccggcgaaga cggggggatt cgttccgttc tgtaaaacag tatttatccg ccagatagtc
     2521 aattcgtgta cgcatactga aaggaattcc gtataaagat tcagcagaca aaactttatc
     2581 agccagtaaa aattaacgga agcattgata ttcctcccga caatcaccgg atgattgttg
     2641 caataacctg cgacataaag gactgaacag ccgtttttaa cgccacgtat tgccctgatg
     2701 aaccaggccg ggccacccga aaacatcttt ccacccggca ttgtccgcca gcacttatca
     2761 ccgaccaggt ctgttgcagt aaatccacca gttgcgcaca ttcttcatca gccatgacgc
     2821 cgactcgcgg atggcaaata ctcgtctggc tgagcagcaa cagacatcgt atctgtgctc
     2881 cttcatgttg ttcccgcaac cagcgtaata ccaccggcgc tgatgggctg accgtgtgat
     2941 tatgctccgt aatacgttcg tgtacccgtc ggtgaaagga actacaaaat gtctgaatct
     3001 gttgaccata gattccttct gccgaataga gcgtgaagtg aaatgccaac agatttggcc
     3061 agtggtccac cgcctgctga acaaaacgcc ggatttcccc cggctctgaa attaaggctt
     3121 cggttatttg cacgaaatgt tccttttcaa cccctgttga attttgtcaa gccggtgcag
     3181 acacagtaaa gcgcgcatca acacctgaaa ggtacgatac aaacacaagt tacaaacaat
     3241 aagaacggaa ggacaattaa tcagtgagga ataataacta aattaaccaa aagaatgtta
     3301 ctgacaatac taccaatact taaatagcaa tatattaatt aatgtaagaa cagatgcccg
     3361 gtaggacata tacacaatac atccacaaac acactctttt cattaaatca atcacctgat
     3421 taaaccatga aagtgtgatg taagtcaaaa ttactgatac tatcaattga taatgattat
     3481 ctctatcatt atcgtgaaat t
//
LOCUS       DAZGJW010000287         3461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZGJW010000287 DAZGJW010000000
VERSION     DAZGJW010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.290"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(120..1658)
                     /gene="cadC"
                     /locus_tag="ACHKIK_005078"
     CDS             complement(120..1658)
                     /gene="cadC"
                     /locus_tag="ACHKIK_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO9086050.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(1918..2032)
                     /locus_tag="ACHKIK_005079"
                     /pseudo
     CDS             complement(1918..2032)
                     /locus_tag="ACHKIK_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2407..2556)
                     /locus_tag="ACHKIK_005080"
     CDS             complement(2407..2556)
                     /locus_tag="ACHKIK_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO9086051.1"
                     /translation="MLIAWLQDNIDCESGIIFDNDEDKTDSAALLPCIEQAREARYFP
                     SVLSA"
     gene            complement(2556..3046)
                     /locus_tag="ACHKIK_005081"
                     /pseudo
     CDS             complement(2556..3046)
                     /locus_tag="ACHKIK_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
     gene            complement(3138..3461)
                     /locus_tag="ACHKIK_005082"
                     /pseudo
     CDS             complement(3138..3461)
                     /locus_tag="ACHKIK_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
BASE COUNT          963 a          732 c          747 g         1019 t
ORIGIN      
        1 gttaaattta tgtaataaaa attatgcggc aaataaattg ccgcaacata ttataccaac
       61 agaaacatac aaaaactcaa caacaaatat tcccgagcat aaatcaaccc gggagttact
      121 tattctgaag cgagaaattt gtcgagataa ggtacaacat aaggaacaga agtctggaat
      181 ataccatttt caatccagta aagggtgttt gcccctgggc gtaaattaaa ggcggtgaga
      241 tatgcatcag ccgcttcccg gttcatcccc ttcatttcat aaaccttgcc aagcaacaca
      301 taatttagcc aggacatttc aagatcaatg ccagtattta tcgcctggta agactcatct
      361 gttttacctt ttaccagagc actgaccgct tttatttgat atataatgga caggttattc
      421 aattccggca gtgtaacaat gttgtctatt tctgtgttca gtgctgctaa ttgtttttca
      481 tctaaaggat gttgagaatg gcgcacgata tcaactaatg ctttttctgc tctcgcgtag
      541 gtaaattctg gggatgattg aacaatctca cctaataatt cactggcacg attcaatgat
      601 ttatcatcgc catgcagtaa ataatcatgt gcctgataaa aattagttaa taacgcacca
      661 cgatgaggca atatttgctg gagcgtctcc tgcattcgtt gtggccacgg ttggtttaac
      721 gcttttgata aactctccag taaatcattt tgaatcgcca gctgattacc gttagtgatg
      781 acataacgtt tatccagcat ggttgaacca tctgcattgt ctaccaattt tatcgacata
      841 aagcattgtt gagcacggta ttggcgctga ttaacaaacg caatagataa tgttttaccg
      901 gaactgctcg gttcatcaat gttgtagttg attttgtcat gcaccataaa ggtggagaag
      961 gtgttaagtg atgtcgccac caaatcaccc acgcctatcg cgtaagagag ctgatacggg
     1021 gaactccagc tgttacaact tttatttacc atattaatgt caatatcgcg tggattgagc
     1081 aaaatacgcg atttgctcat aggaagacgt gtatcaagag ttgaaaacgc taccagtgct
     1141 acacagatac ctaacgacaa caggaaaaaa aaccataccc aaaaggtagt gaatcgtttg
     1201 cttttaactg gggattgttc aggtggcgtt gcggtgtttt gaatgttaag actgtgggag
     1261 ggagaatctg tggcaggaac cgcctctggt atagggggag gcgaagatag cattatttcc
     1321 tctccctctt cttcgctgta ccagataacc ggcaccatta atttatagcc gcgctttggt
     1381 acagtagcga tatagacagg actatcttca tcattatctt ttaatgactt acgtagttct
     1441 gagatactct gcgtcacaac atgattggtg acaatacttc tcttccagac attatcgata
     1501 agttcatccc tgctaagtac ttcgccactg tgttgagcaa agaaaaccag aagatcgatt
     1561 aatctcggct caagggtaag ttgacgccca ttgcggctaa tttggtttat ggacggagta
     1621 acaagccatt cgccaacgcg aactacaggt tgttgcataa tagaaactca ttcgaaaagg
     1681 gaatgatgca atgataattg ccacaaccta tttttaccat ctatagatgg gtttatttac
     1741 atattattgg tgaatgcaag acgttatttt taccagccat aaacttcttg attacatagt
     1801 attacgaaag gattttactg agaaccagaa gtaatattcc ttaccatcaa aattcatcat
     1861 ctttgccaaa gaaaaatgtt cagaaaataa tccatggaaa attgtccgga gcgcttacta
     1921 ttttaatgga ttgttagtct ttgcatgagc aagcggactg atacatttct ctttgttctc
     1981 attcagaaaa tctcatcagt cggcgttcag accggtgttg tgctttatgg atgcgttacg
     2041 ctcctgatga cgtcatttga cgttcaacaa catcacgggg ccgcacgaca tttcacgtca
     2101 gttagtgcta tcgctcagga acaaattttc ccgaattggg atatgcccgc aaattgctgg
     2161 tgatgtgggg agaatctggt tgagttcggg agaattgatt tggaggcaga acgcttaaat
     2221 tgtggcgtcc agaaacgaaa aacggacctc cgtggaggtc cgttgatatg aatttggtgc
     2281 ggactcggga atcgaaaatt tcctttatca tattgatatt aatgcaattt gtttggtttg
     2341 attaaaaatg caccctcaat tatacccgca gtcccttttg atatcgaaag ggaatcacac
     2401 tcgggatcag gctgagagca cagaaggaaa gtacctggct tccctggcct gttcaataca
     2461 gggcagcagt gctgccgaat ccgttttatc ctcatcgttg tcaaagataa ttcccgattc
     2521 gcagtcgata ttgtcctgca gccacgcaat gaggatatac ggagaagcac atccgtatca
     2581 tcagtgtgac gactgcggcg gccatccatc cagtcatcgg ttcgtctgag aatgacgtac
     2641 aactgcgcac gcgacacccg gagacaacgg ctgactaagc ttactcccca tccccgggca
     2701 ataagggcgc gtgcgctatc cacttttttg cccgtccata ttcaacggct tctttgagga
     2761 gttcattttc catcgttttc ttgccgagca ggcgctggag ttctttaatc tgcttcatgg
     2821 cggcagcaag ttcagaggca ggaacaacct gttctccggc ggcgacagca gtaagacttc
     2881 cttcctggta ttgcttacgc cagagaaata actggctggc tgctacacca tgttgccggg
     2941 caacgaggga gaccgtcatc cccggttcaa agctctgctg aacaattgcg atcttttcct
     3001 gtgtggtacg ccgtctgcgt ttctcaggcc ctaagacatc aatcatctgt tctccaatga
     3061 ctagtctaaa aactagtatt aagactatca cttatttaag tgatactggt tgtctggaga
     3121 ttcagggggc cagtctagtt ttttgttatg ttggctgagt gacaggatgt taatctgatg
     3181 tcttctactt catgaagatt atgttctacc agacgattga cgatatcagt tggtgataaa
     3241 aattcatctc cacacttaag aagaggtagg ccagcagaac catcccctgg cagataaatt
     3301 ttattgtatt tttctggtgt taaatccttt ggtaggatag ttttcatctc tgttgccgca
     3361 atacttatag ctgctgcggc agcaacatcg gtgttacttg attgtagata acttctgttt
     3421 gcaccaggaa aggtaaactc tgttttcttt ttatcaaaat c
//
LOCUS       DAZGJW010000288         3424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZGJW010000288 DAZGJW010000000
VERSION     DAZGJW010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.291"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..788
                     /gene="yibB"
                     /locus_tag="ACHKIK_005083"
     CDS             <1..788
                     /gene="yibB"
                     /locus_tag="ACHKIK_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFO9086052.1"
                     /translation="FREKLARSVDVYFSYFERLAALENEMIIFTSPDLKPRVEAIRNG
                     KPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPRQLKNPEYWSPEYVLVCNLKAY
                     FVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFDESKMHLFTIKKGLTVTS
                     QQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLESQKITLNNNIVDDDQGIFVMCY
                     YKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIFLSRK"
     gene            1063..2259
                     /gene="kbl"
                     /locus_tag="ACHKIK_005084"
     CDS             1063..2259
                     /gene="kbl"
                     /locus_tag="ACHKIK_005084"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO9086053.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            2269..3294
                     /gene="tdh"
                     /locus_tag="ACHKIK_005085"
     CDS             2269..3294
                     /gene="tdh"
                     /locus_tag="ACHKIK_005085"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO9086054.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
BASE COUNT          858 a          745 c          897 g          924 t
ORIGIN      
        1 ggttccgtga aaaactagct cgttcagttg atgtttattt tagttacttt gagcgtctgg
       61 ccgcacttga aaatgagatg attattttca cttctcccga tttgaaacca agggttgaag
      121 cgattcgcaa cggaaaacca acaacggtta tcgttatcga tataaaaaag aaatttagat
      181 atatcagaag tcgtatcgaa aaaattcaaa aagatgagtc gtttacaaat agacttgagc
      241 ctcgacaatt aaaaaaccca gagtactggt caccagagta tgttttggta tgtaacctta
      301 aggcctattt tgtaaataaa gctatcaaca tgggcttagt gaaaacgcct cttgttgcgt
      361 ggatagattt tggctattgc cgtaagccaa atgtaactcg aggattgaaa atttgggatt
      421 tcccatttga tgaaagtaag atgcatcttt tcactataaa aaaagggctt actgtcacat
      481 ctcaacaaca ggtttttgat ttcatgattg gcaatcatgt atatattatt gggggcgcaa
      541 ttgttggttc acagcataaa tggaaagaat tctataaatt agttctggaa agtcaaaaaa
      601 taacactaaa taataacatt gtggatgatg accagggcat ttttgtcatg tgctattaca
      661 aacgccccga tctcttcaac ctaaactatc tggggagagg aaaatggttc gatttgtttc
      721 gctgcttcag gagtaacact ttaggggcaa aaatgcaggc gctgagaatt ttcctttcaa
      781 gaaaataggt ttccctccat tagtgggtag cgttttgata ccaactgaga taggaaaaat
      841 gatgctttgg catcattttt tctttttata tcaatgcagt aaaatcaatc atcattatgc
      901 ccagccaaaa ctgtacagtt tttctactat ctgtaggtgt tgagagacat caagttacac
      961 gttatttatc ctgaattttg cagaagtgtt aacgcgtcat ctcgtcgcga cctataagtt
     1021 tgggtaatat gtgctggaat ttgccctgtc tggagaatcg caatgcgtgg agaattttat
     1081 cagcagttaa ccaacgatct ggaaaccgca cgggcggaag ggttgtttaa agaagagcgc
     1141 attattacgt ctgcgcagca agcagatatc actgtggctg atggaagcca cgtcattaac
     1201 ttttgtgcca acaactatct cgggctggcg aatcatcctg atctgattgc ggcggcaaag
     1261 gcgggaatgg attctcacgg tttcggcatg gcttcggtgc gttttatttg cggcactcag
     1321 gacagccata aagagcttga acaaaaactg gcggccttcc tggggatgga agatgcgatt
     1381 ctctactctt cctgctttga tgctaacggt ggcctgtttg aaacgctgct gggcgcggaa
     1441 gacgccatta tctccgacgc actgaaccac gcgtctatta ttgatggtgt gcgtctgtgt
     1501 aaagctaaac gctatcgcta tgccaacaac gatatgcagg agcttgaagc tcgtctgaaa
     1561 gaagcgcgtg aagccggtgc gcgtcatgtg ctgattgcca ccgatggtgt attctcgatg
     1621 gacggcgtga ttgccaatct gaagggcgtt tgcgatctgg cagataaata cgatgccctg
     1681 gtgatggtag acgactccca cgcggtcggt tttgtcggtg aaaatggtcg tggttcccat
     1741 gaatactgcg atgtgatggg ccgggtcgat attatcaccg gtacgcttgg taaagcgctg
     1801 ggcggggctt ctggtggtta taccgcggcg cgcaaagagg tggttgagtg gctgcgccag
     1861 cgttctcgtc cgtacctgtt ctccaactcg ctggcaccgg ccattgttgc cgcgtccatc
     1921 aaagtactgg agatggtcga agcgggcagc gaactgcgtg accgtctgtg ggcgaacgcg
     1981 cgccagttcc gtgagcaaat gtcggcggcg ggctttaccc tggcgggagc cgatcatgcc
     2041 attattccgg tcatgcttgg cgatgcagta gtggcgcaga aatttgcccg tgagctgcaa
     2101 aaagagggca tttacgtcac cggtttcttc tatccggtcg ttccgaaagg tcaggcgcgt
     2161 attcgtaccc agatgtctgc ggcgcatacc cctgagcaaa ttacgcgtgc agtagaagca
     2221 ttcacgcgta ttggtaaaca actgggcgtt atcgcctgag gatgtgagat gaaagcgtta
     2281 tccaaactga aagcggaaga gggcatctgg atgaccgacg ttcctgtacc ggaactcggg
     2341 cataacgatc tgctgattaa aatccgtaaa acagccatct gcgggactga cgttcacatc
     2401 tataactggg atgagtggtc gcaaaaaacc atcccggtgc cgatggtcgt aggccatgaa
     2461 tatgtcggtg aagtggtagg tattggtcag gaagtgaaag gcttcaaaat cggcgatcgc
     2521 gtttctggcg aaggccatat cacctgcggt cactgtcgca actgtcgtgg cggtcgtacc
     2581 catctgtgcc gcaacacgat cggcgtcggc gttaaccgtc cgggctgctt cgccgaatat
     2641 ctggtgatcc cggcgttcaa cgccttcaaa atcccagaca atatctccga cgacctggct
     2701 tccatttttg atcccttcgg taacgccgtg cataccgcgc tgtcgttcga tctggtgggc
     2761 gaagatgtgc tggtttctgg tgcaggtccg ataggtatta tggctgcggc ggtggcgaaa
     2821 cacgttggtg cacgcaatgt ggtgatcacc gatgttaacg aataccgcct tgagctggcg
     2881 cgcaaaatgg gtatcacccg tgcggttaac gtcgcgaaag aaaaccttaa tgatgtgatg
     2941 gctgaactgg gcatgaccga aggctttgat gtcggtctgg aaatgtccgg cgcgccgcca
     3001 gcgtttcgta ccatgctcga taccatgaac cacggcggtc gtattgcgat gctgggtatt
     3061 ccaccgtccg atatgtctat cgactggacc aaagtaatct ttaaaggctt gttcattaaa
     3121 ggtatttacg gtcgtgagat gtttgaaacc tggtacaaga tggcggcgct gattcagtca
     3181 ggtctggatc tctcgccgat cattacccat cgtttctcta tcgatgattt ccagaagggc
     3241 tttgacgcta tgcgttcggg ccagtccggg aaagtaattc tgagttggga ttaacacgaa
     3301 caagggctgg tattccagcc cttttgcctg aggataatcc gttaaatatg taaaatcctg
     3361 tcagtgtaat aaagatatcg taattgcgct gatctcttat atagctgctc tcattatctc
     3421 tcta
//
LOCUS       DAZGJW010000289         3405 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZGJW010000289 DAZGJW010000000
VERSION     DAZGJW010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.292"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            10..126
                     /locus_tag="ACHKIK_005086"
     CDS             10..126
                     /locus_tag="ACHKIK_005086"
                     /inference="COORDINATES: protein motif:HMM:NF018315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086055.1"
                     /translation="MINHIIALVDVLNKKSYASNVIKFLITGDILSESKRAP"
     gene            176..445
                     /locus_tag="ACHKIK_005087"
     CDS             176..445
                     /locus_tag="ACHKIK_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO9086056.1"
                     /translation="MEIRDKKAKYLEEKGFYRRAADRWAEVMVLLSSDAERKLAAQKR
                     AFCINKSLRNNLQADNYSDIKQGVYKAYKKMGLVNEKIFRNYKEN"
     gene            complement(678..1482)
                     /locus_tag="ACHKIK_005088"
                     /pseudo
     CDS             complement(678..1482)
                     /locus_tag="ACHKIK_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(1722..2300)
                     /locus_tag="ACHKIK_005089"
     CDS             complement(1722..2300)
                     /locus_tag="ACHKIK_005089"
                     /inference="COORDINATES: protein motif:HMM:NF017150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFO9086057.1"
                     /translation="MSEKTSTQKHKSREKGLSLIESAMVLALSAVVIAAVLYFYNNSI
                     ENKKVNDGISRLQTIIAATNGFIASNASAFDSNIGNFIKAVSLKTGIPTINPGDGTTQ
                     LVDENGRGIQIWSVSSSRYYVIEVQTSNIPTCVKYASMNMGTMYYQKPDIYIGNKKIG
                     TANDVSSANTACQKADQQRNDANIKIRYSLKY"
     gene            complement(2734..>3405)
                     /locus_tag="ACHKIK_005090"
     CDS             complement(2734..>3405)
                     /locus_tag="ACHKIK_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc31 family transposase"
                     /protein_id="HFO9086058.1"
                     /translation="TQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTCLPNL
                     LERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANADTALISSA
                     LRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDNSPMERFF
                     RSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPHHYNGGLTPEESENRYHFYCKT
                     VASIT"
BASE COUNT          934 a          747 c          754 g          970 t
ORIGIN      
        1 ttctcacatg tgattaatca tataatagct ttagttgatg ttcttaataa gaaaagttac
       61 gcaagcaatg ttataaaatt tttgataacg ggtgatattc tttctgaatc aaaaagagca
      121 ccatagttta tcaagtaaaa aaatgataca tacactacta taaacaaagg taattatgga
      181 aataagagat aaaaaggcga agtacttgga agaaaaaggg ttttatagac gagctgcaga
      241 tcgctgggca gaggtaatgg tgcttttatc tagtgacgct gagagaaaat tggcagctca
      301 aaagcgtgca ttctgcatta ataaatctct acgtaataat ctgcaagcag ataactatag
      361 cgacataaaa caaggagttt ataaagcata taagaaaatg gggcttgtaa atgaaaaaat
      421 ttttagaaac tacaaggaaa attaaattat aacgtagatt gagtctaaat aactgttcgg
      481 aatagtagat ctctcagggg gactcagtcc ggtttatgct agttggtcaa tcgtcgcata
      541 tcccaaatca ccaaccggac tgagcaatgc agataatagc accaattccc agaacagaac
      601 gacgcctgaa gcagaaaacg ttccataaaa caaaggacaa aaagagcctg tttaagattc
      661 tgtgtaaatg ccttttctca gaagtgaccg tccaggcggt caccaaactc gataataaag
      721 cggctcattg ccattcgcca gtccttcaac ggcatcgtcc atttctggga cgcagactgg
      781 attgccagcc acacaggcga tgaggatatc gttcagaccg cggtttttca gttcagtcag
      841 cacattgagc cagaacttcg ccccttcatt ttcggccagc cacataccca gcagttcttt
      901 ctgaccttcg atattgctgc ccagtgccag gaacaccgat ttgttgatga cgcgactgtc
      961 ctgccgaact ttcaggacga tacagtcaag ataaacaatg gggtaaacag catccagtgg
     1021 tcggttttgt cattctacaa cctgctccat cacggcatcg gtaacctttg atatcagtgc
     1081 cggtgaaaca tctgcgtcat acagttcttt gaacgcagca gctatctcac gggtggtcat
     1141 ccctttggca tacaacgaga ggatctggtt atccatcccg gtaatacggg tctgattttt
     1201 ctttaccagt tgtggttcga aggtaccgtc acgatcgcgc ggagtacgca gttccagtgg
     1261 accgtcgcct gtgataacgg tctttgtgga ataaccgttg cgggagttag ctcctagtct
     1321 ggactgattt ttctcatacc caagatggtg tgtcatctct gcattgagag cggcttcaac
     1381 gctgagcttt ttcagcagcc gatcaaactg actgaggtct tcaggggttt tgaggttttt
     1441 ggccagttcg ttagccagag cctgtaactg tttttcgtcc ataaattaac cttcatttga
     1501 tgctggattg aacatatcaa aatcaggcaa ttacacaaat ctatgtacag gctctgcgaa
     1561 taagtgatcg atcatgtcgg aatatgcaaa aacccgaaat caccagttgc cacattgaac
     1621 ggcgctggtg atttcgggtt cgtcacttta tggataccat caacccattc cccggagaaa
     1681 gcatgggctg gctaaagtgt ggcggcatta agagcagtta tttagtattt taatgagtat
     1741 cgaatcttta tatttgcatc attccgttgt tggtccgcct tctgacaagc tgtgttggca
     1801 gaagaaacgt cgttagcggt tcctattttt ttattaccta tatatatatc aggtttttga
     1861 taatacatgg tccccatatt catactggca tatttgacac aggtgggaat gttgctggtt
     1921 tgaacttcaa taacatagta tctgctactt gatacagacc aaatttgaat tcccctgcca
     1981 ttctcgtcga caagctgtgt ggtgccatcc cctggattta tagtaggtat tcctgttttt
     2041 agagatacag ctttaataaa gttgcctatg ttactgtcaa aagcagaagc gttgctcgca
     2101 atgaatccat tagtggcagc aataatagtt tgcaatctgg atatgccatc atttactttt
     2161 ttattctcaa ttgaattgtt ataaaaataa aggactgcag caattactac agctgataaa
     2221 gctaaaacca ttgctgattc tattagtgac agaccttttt ctcttgattt atgtttttgg
     2281 gttgatgttt tttcgctcat tgctttgcat atagtttagt ggaataaatt ctataattga
     2341 gcatatctca tttgtagata aaacagagca aaaaaataag taaagatata taaaaaagtg
     2401 aactgaaccg ccccggtttt cctggagagt gttttatctg gcgagtaggc aacctgacgg
     2461 ctgcggcttg tcatggtctg aaactaccgc tataaaaaca ggccatatca ttgtctttca
     2521 ggtagttata tggcccgctc agctaaactc cgtaaacaaa aatccggcag acagtcttcc
     2581 cgctcagccc ccggttgcaa tggcgggtgg ttccctgtgc aggcggacct gacaatgaag
     2641 ccggactgtt ttttcccgac ttcggcagtg ccgcagatta ctgtatggag aaagatattc
     2701 cgtacctgtt tgaagaagat gccactgtag tggtcaagta atactggcca cggttttaca
     2761 gtaaaaatgg tatctgttct ctgactcttc cggcgtcagc cctccgttat aatggtgagg
     2821 cctgacgcta ttgtaataat tcagaatata actgctgatt tgctgccggg ccacgtcttt
     2881 gcctgtgtag ccatcggttg gcacccattc tgttttcaga ctgcggaaga agcgttccat
     2941 aggactgtta tcccagcagt ttccccgtcg gctgacactt tgctttatcc tgtaacgcca
     3001 gagaagttgt tgatatttca gtcctgtata ttgacttccc tggtcgctat ggaacataac
     3061 gtcccgcggc tgaccacgca cctcatacgc catccgcagg gcactgctta tcagggcagt
     3121 atcggcattc gctgacaggc tccagccgat aaccctgcgg gcaaaaagat ccatgacgac
     3181 cgccagatag caccagcgat ttcctgccca gatatacgta atatctccgc accataccct
     3241 atctggctcg ggcacagcga actggcgctc aagcagattc ggcaggcagg tatgttcctg
     3301 acgagcattt ttgtactgat gttttccggg ctgacaactg ctcaggttca gatatttcat
     3361 cagacgcccg gcacggtaac ggctcatcgg gacgccgttt tgggt
//
LOCUS       DAZGJW010000290         3401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZGJW010000290 DAZGJW010000000
VERSION     DAZGJW010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.293"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..155
                     /locus_tag="ACHKIK_005091"
                     /pseudo
     CDS             1..155
                     /locus_tag="ACHKIK_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            157..426
                     /locus_tag="ACHKIK_005092"
     CDS             157..426
                     /locus_tag="ACHKIK_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO9086059.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDTDKKLIQCVVRPLQILRADGTWENIGGMK"
     gene            532..1413
                     /gene="nleH"
                     /locus_tag="ACHKIK_005093"
     CDS             532..1413
                     /gene="nleH"
                     /locus_tag="ACHKIK_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase NleH"
                     /protein_id="HFO9086060.1"
                     /translation="MLSPSSVNLGCSWNSLTRNLTSPDSRILSSVRDAAASSDNGAQV
                     KVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNSSPVNRAM
                     ERGAVHSNRPDLPPVDYAPPELPSVDYNSLPVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTNEVRCFNQYYGAGSAEKIYGDNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFIDTTETNVLYDRTRNEFNPIDISSYNISERSWSENQIMQ
                     SYHGGKQDLISVVLSKI"
     gene            1637..2464
                     /gene="cif"
                     /locus_tag="ACHKIK_005094"
     CDS             1637..2464
                     /gene="cif"
                     /locus_tag="ACHKIK_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000652081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector Cif"
                     /protein_id="HFO9086061.1"
                     /translation="MKDITLPPPTSASCLTGAISVNTEAVLSPMQHTSALHVRDFASL
                     CSQNLKANVLLNSDDHEVPIHQKNPAAIMQNIDSNIKQMATDWGMSIEEVEVIIGREK
                     GIVEPSCGVTANAIMKLFLDKDGFSYCFENEQTLSLEQLQERLSCMPECKSFVLRVND
                     GALGHAYIVDIPKGENSCRPAFLYQSDLGEGVTRKLRFEDWMTHKALTPILLDDICNY
                     FSCMSQNKTDLEQIATLFDIDGNVKMLRKENIQYQKHDNFSFQLFEYDTDNIEKTLR"
     gene            complement(2600..3120)
                     /locus_tag="ACHKIK_005095"
                     /pseudo
     CDS             complement(2600..3120)
                     /locus_tag="ACHKIK_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3164..3244
                     /locus_tag="ACHKIK_005096"
                     /pseudo
     CDS             3164..3244
                     /locus_tag="ACHKIK_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT         1014 a          611 c          807 g          969 t
ORIGIN      
        1 gcactcatca ccggactgac ctttcttgac cccaaagatg ccacacgggt tcagggtttt
       61 ttcagcattt gcaggtcagg tttggtgacg ggccgtggca ggatgtcaag gggctggatg
      121 aagtgggcag tgatacaggc agaacaggag aatgacatga atatactaaa aaaacttatg
      181 cagcgtctgt gtggttgcgg aaagcatgat ggccgtgaac acgtgcagtc gcttacagca
      241 caactgcgac tggggccggc agacatcctg gagtcagatg agaatggcat tattccggag
      301 caggacaggg taatcacaca ggtggtgata ctggatacag ataaaaagct gatacagtgt
      361 gtggtaagac cgctgcaaat cctgcgtgct gacgggacgt gggaaaatat tggcgggatg
      421 aagtaacccg acagcttcac aaaaccggag tccggctccg gtttttgttg tcatgtcatg
      481 gtgatgtttg ttaagaaagt aaagattgat tcattttgaa ggttgaaatg tatgctatca
      541 ccatcttctg taaatttggg gtgttcatgg aattctttaa ccagaaacct gacttctcct
      601 gacagtcgta ttttatcctc tgtaagggat gcagctgcta gctcggataa tggggcgcaa
      661 gtaaaggtgg gcaacagaac atatcgtgtt gttgtcactg ataataagtt ttgcgttaca
      721 agagaaagtc atagtggttg ctttactaat ctgttgcaca ggctgggatg gcctaaggga
      781 gagattagca ggaaaattga ggtgatgctg aattcatcgc cagtgaatag ggctatggaa
      841 agaggtgctg ttcattcgaa tagacctgat ttacctcctg ttgattatgc accgccagag
      901 ttaccgagtg tggactataa cagcttgcct gtgcctggta atgttattgg caaagggggt
      961 aacgctgtag tatatgaaga tgctgaggat gcaacaaaag tcctgaagat gtttactaca
     1021 tctcaaagca atgaagaggt aacaaacgaa gttcgttgct tcaaccaata ttatggtgcc
     1081 gggagtgcag aaaaaatata tggcgataat ggcgatatta ttggtattag aatggataaa
     1141 ataaatggag aatcgctttt aaatatttcg tccttgccgg cacaggctga gcatgccatt
     1201 tatgatatgt ttgacaggct tgagcaaaaa ggaattcttt ttatcgatac aacagagaca
     1261 aatgtcttat atgatcgcac gagaaatgaa tttaatccaa tagatatatc atcttataat
     1321 atttctgaac gttcatggag tgaaaatcaa ataatgcaat cttatcatgg aggaaagcaa
     1381 gatcttatta gtgtggtatt aagtaaaatt tagtaatttt atccagtgta gtggatttgt
     1441 tgcatggatg gaggtggtaa aaagtggtgt ggctggcaat ccaggccgcg tcacagaaat
     1501 ggacaatgcc gctgagagac tggcgaatgg catacaggca ccagaaaaat ttcagacatg
     1561 acataatgcc catgccagaa gttctttttg cacctcgcct gtatttatag ataaatatag
     1621 agtgaatgaa tgagatatga aagacattac ccttcccccc ccgacgtccg cgtcctgtct
     1681 gacaggggcc atatctgtaa atactgaagc tgtattatct cccatgcaac acacttcagc
     1741 cttacatgta agagattttg cttccctgtg ctcacagaac ctcaaagcta atgtattgct
     1801 aaatagtgat gaccacgaag tacctataca tcagaaaaat cctgctgcaa taatgcaaaa
     1861 tatcgactct aacatcaaac agatggcaac agactggggg atgtcgattg aggaggttga
     1921 ggttattata gggcgagaga aaggcattgt ggaaccctcc tgcggagtta ccgctaatgc
     1981 tattatgaaa ctatttctgg acaaggatgg cttcagttac tgctttgaaa atgaacagac
     2041 actatcgctc gagcagcttc aggagcgcct gtcctgtatg cctgaatgta agagctttgt
     2101 attacgtgtt aatgatggtg cgcttggtca tgcttacatt gtcgatattc ccaaaggaga
     2161 aaactcttgt cgtcctgcat tcttgtatca gtcagattta ggagagggcg tcaccagaaa
     2221 gttaagattt gaggactgga tgacgcataa agcattgact ccgattttgc tggatgatat
     2281 ttgtaattac ttctcctgca tgtctcaaaa taagacagat ttggagcaga ttgcaacgtt
     2341 atttgatatt gatggaaatg ttaaaatgtt acgaaaggaa aatattcaat atcaaaagca
     2401 tgacaatttt agtttccagt tgtttgagta tgacaccgat aatattgaaa aaacattgag
     2461 ataataaaat cactatgtag ttagcttttg ttaaaactgc aattatttgt aaacataata
     2521 ataagtatta tgacgttaaa atttaaccat acggtaaaat gattcaggaa aaccggttca
     2581 gcatagccta ggctgaactt ttgaggatat caacgaacgg aatttttgaa catttatgag
     2641 tagatcgttg ttgaagggct tcaatgagca tgtcaagttc atcaactgct ggtctacgat
     2701 tacgacggtt tgatttacca atcaaagcaa gtttaagtag atatggacga gcacttttcg
     2761 ccgggtttga tgtgtaatta attccgtata taggtttggc tgcatccaga acactgccaa
     2821 gataactaac atcgtgactg actgttgctg gacctgcacc agcgttgttt ctcagcctgc
     2881 aatgttcaat tacgtcattt tctgtcagtt cagatagttt gatcgcggag atgtcactat
     2941 ccataagcag ttctagcaca tatcttttag tacggtctgc tttacctccg gcatttggtc
     3001 atttaaatat ttgtgtagta agtcacggac tgtaagtccg tcaacagcat ttgatgatga
     3061 aatgccatat agatctaata ccatcacttt ctgtgtgccc catgttttag catgagcatg
     3121 aacgcggcgg ctatctcacg ggtgatcatc cctttggcat actgaaaagt atcgggtgtt
     3181 actgaaccgt gttgatacat caactgcact ggatattgag tggccgataa taccgacgtt
     3241 ataacctata aatgccaatc aggctgaatg ttgaaaggat agaatgaaca gcccgacaca
     3301 caaaaaacgg agtccggctc cggtttttgt gttgccatat aaggcagatg tttgttacag
     3361 ctatttaagt ctggagttca aattaaaata gggagttttg t
//
LOCUS       DAZGJW010000291         3363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZGJW010000291 DAZGJW010000000
VERSION     DAZGJW010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.294"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            65..370
                     /locus_tag="ACHKIK_005097"
     CDS             65..370
                     /locus_tag="ACHKIK_005097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086062.1"
                     /translation="MITTETAANTAAAIAGATGNASKDTGNDNGWDIGGGCEGSHQLG
                     MDASKYSLQYSEKSIGYGMYQGGQMSSIPGTFGGFVGSFVSEGASSATQKEVDGDKK"
     gene            624..1004
                     /locus_tag="ACHKIK_005098"
     CDS             624..1004
                     /locus_tag="ACHKIK_005098"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086063.1"
                     /translation="MVSYGQAVSSWNQYADANNLTPEQKQAGLDKLAKGELPEGANIS
                     KVIVDGYKDGVLIAGAWYLGPAASTGKVVSGAVISAIANGTYQWYDLSKPGNENKSWD
                     YWEVPRQALLERWLQVEIYGRTQG"
     gene            1485..2381
                     /locus_tag="ACHKIK_005099"
                     /pseudo
     CDS             1485..2381
                     /locus_tag="ACHKIK_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081256.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            2378..2608
                     /locus_tag="ACHKIK_005100"
     CDS             2378..2608
                     /locus_tag="ACHKIK_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086064.1"
                     /translation="MKLFIFSLFVIVTSMVSGIAIAELSYFILLIIKYLAYGEVDFRW
                     SEVLRGLRMGCVGGGILGFGIVLFRFLGIKGF"
     gene            2647..2784
                     /locus_tag="ACHKIK_005101"
     CDS             2647..2784
                     /locus_tag="ACHKIK_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C551"
                     /protein_id="HFO9086065.1"
                     /translation="MSGLEGFGTGFRSYVQAQGTLVNNTNLTDETGKVLNPATPEEIK
                     Y"
     gene            2888..>3363
                     /locus_tag="ACHKIK_005102"
     CDS             2888..>3363
                     /locus_tag="ACHKIK_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO9086066.1"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTEL
                     GRNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIS
                     EQKRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIV"
BASE COUNT          959 a          615 c          881 g          908 t
ORIGIN      
        1 gattattata tataacatgg ttaactttat taatttttag gtagttgaga acaatatact
       61 gaatgtgata accactgaaa cagcagctaa tacggcagca gcaatagctg gtgctacagg
      121 taatgccagt aaggataccg ggaatgataa tggctgggat attggtggtg gttgtgaagg
      181 ctcacatcaa ctgggaatgg acgccagtaa atattcactg caatattcgg aaaagtctat
      241 cggatacggt atgtatcaag gcggccaaat gagttctata ccaggaacat ttggtggatt
      301 tgttggttca ttcgtatcag aaggtgcaag cagcgcaacg caaaaagaag ttgatggaga
      361 caaaaaatga aaaaacatct actacttctt attaagttgt tacttatatc tatagctact
      421 tgctctattg gaatagtggt tagtatctgg tttatagctc gggaaccaat accttctgag
      481 gtgtatgtaa aatacggaat aattggcgca gtagttggct tatggggagg tgctgggatt
      541 tggttacaac tctatctaca aatgcgaaga tactatcgta aatagttgtt gttgagaata
      601 atgcgctcag tctgccatca ggaatggtga gttatggtca ggcggtttcc tcatggaatc
      661 agtatgctga tgccaataat ctcaccccgg aacagaagca ggcaggactg gataaacttg
      721 ccaaaggtga gttacctgaa ggtgccaata taagtaaagt gatagttgat ggctataaag
      781 atggcgtatt gattgcaggt gcctggtatt taggacctgc agcatcaaca ggtaaagtag
      841 tcagtggcgc agtcatctca gccattgcca atggtacgta tcagtggtac gacttgagca
      901 aaccaggcaa tgaaaacaag agctgggatt actgggaagt gcctcggcag gcattactgg
      961 agcgctggct ccaggtcgaa atatatggca gaacgcaggg atagcggcag gtggaactct
     1021 atttattgat ggaccagata aaggtgcttt aactggtacg ggagctggtt gggtattcgg
     1081 tactgtagtt ggggtagttg caccgccggt atttagccct gttttaggtc caggttcagt
     1141 gccttcaggt gatattattg gtgctatagg tggtgaattt atcagcaatg cagttaaaga
     1201 tgagataaat ggaacgaaaa aataaagcta ctgtaggttt actgctccta agtattatgt
     1261 acatgatttt ctgctgctta atattcttct tcttgactct gatagtagtt aaatttgtta
     1321 ttagccatga agtgaatatt aagcaatcag atcttgaaaa tattttagtt gctagtgtta
     1381 ttgttggaac tgcagcagca tttagatcgt ggatcttcac caaattggac gaacgcaagg
     1441 ctcgaaaaac gccctcgtca ggatcctgag taatccggac attgggaaat aacgttcttg
     1501 ctggtgccgc aggtgctgcc accggcgagg tggtgggaat gatagccaca cagatgtacg
     1561 gtaagccggt gagtgaactg agtgagacag agaagcagac ggtctcgaca ctgacaacgg
     1621 ttgctgccgg cctggccggt ggtctggtgg gagactcggg ggcttcagcg gtagccggcg
     1681 cacagtcggg taagacaacg gttgagaata atctgctggg tggcagtgaa ttgctccaga
     1741 cggaaaaagc cagggagcat ggtgctgatg ttctctcctg tagtgataat ccttccggtg
     1801 aagcctgtaa gcgggggcag gctgaaaata aagcttatgc agccgcatta gcaacaggca
     1861 gcgttgccct gttaccgggg agtgcccagg ctatgtggtt acttggtgcc gggacaaatg
     1921 ccgggatgca gtatgcagat agtggtgaaa taaacccggt caactcagtg gcagcaggct
     1981 ggataaatgt gataaccatg gggcagggct ggaaagggac aatagcttgg aatgcggcag
     2041 gtggagcatt aaccaacgcc ataaacggag atgatcctct tactggggcg attacaaatg
     2101 gagcgggggc cgggtttgga tatggggttg gtaattatgt ggtcaagcct gctgcaaata
     2161 caatcggtaa atggataacc ggtggctgga atcctaaatt cgatccgact ctactaaaat
     2221 atactgaagt taaagggcag ttaggtctat ctaaagaaat ggtaccgagt aagattccgg
     2281 gggctgcagg taatatgggg gcttctatta catcagaaat gacggggact gaactacaaa
     2341 aagtcattaa tgataaattg aagaaaggag agcgtgaatg aaactattta ttttcagtct
     2401 ctttgtcata gttacctcca tggtttcagg aattgcaatt gctgagttgt cttatttcat
     2461 attgcttatc attaaatact tagcgtatgg ggaagttgat tttagatggt ccgaagtttt
     2521 aagaggtcta aggatggggt gtgtgggagg tggaattcta ggttttggta tagtcttgtt
     2581 tcgctttctt ggcataaaag gattttaagt gaagataagg tacgatttgt tgttgagaat
     2641 aattcaatgt ctggcctgga agggtttggg accggcttcc ggagttatgt tcaggcccag
     2701 gggacgctgg taaataatac caatctcact gatgaaactg gcaaagtgct gaatccggca
     2761 acgccagagg aaattaagta ctgatgaatc ccctaatgat tttggtaaaa atcattaagt
     2821 taaggtggat acacatcttg tcatatgatc aaatggtttc gcgaaaaatc aataatcaga
     2881 caacaagatg tgcgaactcg atattttaca cgactctctt taccaattct gccccgaatt
     2941 acacttaaaa cgactcaaca gcttaacgtt ggcttgccac gcattacttg actgtaaaac
     3001 tctcactctt accgaacttg gccgtaacct gccaaccaaa gcgagaacaa aacataacat
     3061 caaacgaatc gaccgattgt taggtaatcg tcacctccac aaagagcgac tcgctgtata
     3121 ccgttggcat gctagcttta tctgttcggg caatacgatg cccattgtac ttgttgactg
     3181 gtctgatatt agtgagcaaa aacgacttat ggtattgcga gcttcagtcg cactacacgg
     3241 tcgttctgtt actctttatg agaaagcgtt cccgctttca gagcaatgtt caaagaaagc
     3301 tcatgaccaa tttctagccg accttgcgag cattctaccg agtaacacca caccgctcat
     3361 tgt
//
LOCUS       DAZGJW010000292         3320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZGJW010000292 DAZGJW010000000
VERSION     DAZGJW010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.295"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            337..1653
                     /gene="shiA"
                     /locus_tag="ACHKIK_005103"
     CDS             337..1653
                     /gene="shiA"
                     /locus_tag="ACHKIK_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO9086067.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDVQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFARLADRFGRRRVYITGALIGMLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAITALLMKDNQRA"
     gene            1755..3209
                     /gene="amn"
                     /locus_tag="ACHKIK_005104"
     CDS             1755..3209
                     /gene="amn"
                     /locus_tag="ACHKIK_005104"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO9086068.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          763 a          812 c          803 g          942 t
ORIGIN      
        1 tcaacaatga tactaaaaac ataactaacc tcgcctccct actggttata atgcaatgca
       61 gtctatcaga atcatcaggg tgcaattttg tgcatatgcg gacttttatg tttcatatct
      121 cgaacctgtg ggccctctgc ttaatcctta aacaacacca gcaactcctg cgcttttatc
      181 ttccatcgaa tttttcatgt tgccgctaat cagccataaa atcatttgca gatgcgctct
      241 gtcgaggtag tctcataagg ttcgtttata gatcgacggc aatgtgagtt accttttcca
      301 tactaattat aaaaagacag tacaaacagg atcattatgg actccacgct catctccact
      361 cgtcccgatg aagggacgct ttcgttaagt cgcgcccgac gagctgcgtt aggcagtttc
      421 gctggtgccg tcgtcgactg gtatgatttt ttactctatg gcattaccgc cgcactggtg
      481 tttaatcgcg agtttttccc gcaagtaagc ccggcgatgg gaacgctcgc cgcatttgcc
      541 acctttggtg tcggcttcct tttccgaccg ctcggcggtg tcattttcgg tcactttggt
      601 gaccggctgg gacgtaagcg catgttaatg ctgaccgtct ggatgatggg catcgcgaca
      661 gccttgattg gtattcttcc ttcattctcg accattgggt ggtgggcacc tattttgctg
      721 gtgacactgc gtgccattca gggatttgcc gtcggcggcg aatggggagg cgcagcgttg
      781 ctttccgttg aaagtgcgcc gaaaaataaa aaagcctttt acagtagcgg tgtacaagtt
      841 ggctacggag taggtttact gctttcaacc ggactggttt cattgatcag tatgatgacg
      901 actgacgtac agtttttaag ctggggctgg cgcattcctt tcctgtttag catcgtactg
      961 gttctgggag cattgtgggt gcgcaatggc atggaggagt ccgcggaatt tgaacaacag
     1021 caacataatc aagcggccgc gaaaaaacgc atcccggtta tcgaagctct gttacgacat
     1081 cccggtgctt tcctgaagat tattgcgcta cgactgtgcg agttgctgac gatgtacatc
     1141 gttaccgcct ttgcacttaa ttattcaacc cagaatatgg ggttaccgcg cgaacttttc
     1201 cttaatattg gtttgctggt aggtggatta agctgcctga caattccctg ttttgccagg
     1261 cttgccgatc gttttggtcg ccgcagggtt tatatcacag gtgcgttaat cggaatgttg
     1321 agcgcatttc ctttctttat ggcgcttgaa gcacaatcta ttttctggat agttttcttc
     1381 tccataatgc tggcaaacat tgcgcatgac atggtggtgt gtgtgcaaca accgatgttt
     1441 accgaaatgt ttggtgccag ttatcgctat agtggcgctg gagtcggtta tcaggttgcc
     1501 agtgtggttg gtggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc
     1561 gggaactggc atagcgtcgc catttatttg ctggctggat gcctgatttc cgcaataacc
     1621 gctttgttga tgaaagacaa tcaacgcgct tgatagcctg gcgaagatca tccgatcttc
     1681 gccttacact tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg
     1741 aacaggagac acacatgaat aataagggct ccggtctgac cccagctcag gcactggata
     1801 aactcgacgc gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaactata
     1861 ttacaagtgg cgaattacct gatgaaaacg cccgcaaaca aggtcttttt gtctatccat
     1921 cactgaccgt aacctgggac ggtagcacaa ccaatccccc taaaacgcgc gcatttggtc
     1981 gttttactca cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt
     2041 atctcaatga acaacttacg ttgctgtatc aggattatgg tgcgcatatc tcggtgcaac
     2101 cctcgcagca tgaaatccct tatccttatg tcattgatgg ctctgaattg acacttgatc
     2161 gctcaatgag cgctgggtta actcgctatt tcccgacaac agaactggcg caaattggcg
     2221 atgaaactgc agacggcatt tatcatccaa ctgaattctc cccgctatcg cattttgatg
     2281 cgcgccgcgt cgatttttcc ctcgcgcggt tgcgccatta taccggtacg ccagttgaac
     2341 attttcagcc gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt
     2401 ggggatgcag ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg
     2461 ggaactggat caccgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga
     2521 aaaaacacca gatgcccgcg tggcatttaa ttaccgccga tggtcagggt attactctgg
     2581 tgaatattgg cgtgggaccg tcaaatgcta aaaccatctg cgatcatctg gcagtactac
     2641 gcccggatgt ctggttgatg attggtcact gtggtggctt acgtgaaagc caggccattg
     2701 gcgattatgt acttgcacat gcttatttac gcgatgatca cgttcttgat gcggttctgc
     2761 cgcccgatat tcctattcca agcattgctg aagtgcaacg tgcgctttat gacgctacca
     2821 agttagtcag cggcaggccc ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg
     2881 taaccacaga tgacaggaac tgggaattac gttactcagc ttctgcactt cgttttaact
     2941 taagccgggc cgtggcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt
     3001 tccgcgtgcc atacgggaca ctactgtgtg tttcagataa accgttgcat ggcgagatta
     3061 aacttcccgg ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg
     3121 gcattcgggc aatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac
     3181 gaacctttaa tgagccgccg ttccgataat aagttctgaa ccttcttatc aaaaatagat
     3241 gtgaaggatt aagtaagaca tctggcaaat agcccgcaat aaacgcgggc tgtttgcttc
     3301 cttgggcgga tacgagtttt
//
LOCUS       DAZGJW010000293         3293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZGJW010000293 DAZGJW010000000
VERSION     DAZGJW010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.296"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1820
                     /locus_tag="ACHKIK_005105"
     CDS             <1..1820
                     /locus_tag="ACHKIK_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO9086069.1"
                     /translation="NYYLPKESAYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARV
                     GYSKMLLGVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGK
                     KHRDNTLTMKRFTNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFL
                     GDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDK
                     ETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSS
                     GEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIG
                     ERPDAEVMAERKEHYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQII
                     MGRHDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDIGGIDPTIVYERSKKHGLFRV
                     IPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVH
                     FPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISI
                     SRWQLDLSALLASLQEEDGAATNKKTLADYARALSGEDE"
     gene            1817..2023
                     /locus_tag="ACHKIK_005106"
     CDS             1817..2023
                     /locus_tag="ACHKIK_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO9086070.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2020..>3293
                     /locus_tag="ACHKIK_005107"
     CDS             2020..>3293
                     /locus_tag="ACHKIK_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9086071.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSNEQRDKLAGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVV"
BASE COUNT          766 a          843 c         1004 g          680 t
ORIGIN      
        1 ccaattacta tctcccgaaa gaatccgcat accaggaagg gcgctgggaa acactgccct
       61 ttcagcgggc catcatgaat gcgatgggca gcgactacat ccgcgaggtg aatgtggtga
      121 agtctgcccg tgttggttat tccaaaatgc tgttgggtgt ttatgcctac ttcatagagc
      181 ataagcagcg caacaccctt atctggttgc cgacggatgg cgatgccgag aactttatga
      241 aaacccacgt cgagccgacc atccgtgata ttccatcgct gctggcgctg gccccgtggt
      301 atggcaaaaa gcaccgggat aacacgctca ccatgaagcg tttcaccaat gggcgtggtt
      361 tctggtgcct gggcggtaaa gcggcaaaaa actaccgtga aaagtcagtg gatgtggcgg
      421 gttatgatga acttgctgcc tttgatgatg atattgaaca ggaaggctct ccgacgttcc
      481 tgggtgacaa gcgtattgaa ggctcggtct ggccaaagtc catccgtggc tccacgccca
      541 aagtgagagg cacctgtcag attgagcgtg cagccagtga atccccgcat tttatgcgtt
      601 ttcatgttgc ctgcccgcac tgcggggagg agcagtacct taaatttggc gataaagaga
      661 cgccgtttgg cctcaaatgg acgccggatg atccctccag cgtgttttat ctctgcgaac
      721 ataatgcctg cgtcatccgc cagcaggagc tggacttcac tgatgcccgt tatatctgcg
      781 aaaagaccgg gatctggacc cgtgatggca ttctctggtt ttcgtcatcc ggtgaagaga
      841 ttgagccgcc ggacagtgtg acctttcaca tctggacggc gtacagcccg ttcaccacct
      901 gggtgcagat tgtcaaagac tggatgaaaa cgaaagggga tacgggaaaa cgtaaaacct
      961 tcgtgaacac cacgctcggt gagacgtggg aggcgaaaat tggcgaacgt ccggatgctg
     1021 aagtgatggc agagcggaaa gagcattatt cagcgcccgt tcctgaccgt gtggcttacc
     1081 tgaccgccgg tatcgactcc cagctggacc gctacgaaat gcgcgtatgg ggatgggggc
     1141 cgggtgagga aagctggctg attgaccggc agattattat gggccgccac gacgacgagc
     1201 agacgctgct gcgtgtggat gaggccatca ataaaaccta tacccgccgg aatggtgcag
     1261 aaatgtcggt atcccgtatc tgctgggata ttggcgggat tgacccgacc attgtgtatg
     1321 aacgctcgaa aaagcatggg ctgttccggg tgatccccat taaaggggca tccgtctacg
     1381 gaaagccggt ggccagcatg ccacgtaagc gaaacaaaaa cggggtttac cttaccgaaa
     1441 ttggtacgga taccgcgaaa gagcagattt ataaccgctt cacactgacg ccggaagggg
     1501 atgaaccgct tcccggtgcc gttcacttcc cgaataaccc ggatattttt gatctgaccg
     1561 aagcgcagca gctgacagct gaagagcagg tcgaaaaatg ggtggatggc aggaaaaaaa
     1621 tactgtggga cagcaaaaaa cgacgcaatg aggctctcga ctgcttcgtt tatgcgctgg
     1681 cggcgctgcg catcagtatt tcccgctggc agctggatct cagtgcgctg ctggcgagcc
     1741 tgcaggaaga ggatggtgca gcaaccaaca agaaaacact ggcagattac gcccgtgcct
     1801 tatccggaga ggatgaatga cgcgacagga agaacttgcc gctgcccgtg cggcactgca
     1861 tgacctgatg acaggtaaac gggtggcaac agtacagaaa gacggacgaa gggtggagtt
     1921 tacggccact tccgtgtctg acctgaaaaa atatattgca gagctggaag tgcagaccgg
     1981 catgacacag cgacgcaggg gacctgcagg attttatgta tgaaaacgcc caccattccc
     2041 acccttctgg ggccggacgg catgacatcg ctgcgcgaat atgctggtta tcacggcggt
     2101 ggcagcggat ttggtgggca gttgcgggcg tggaacccac cgggtgaaag tgtggatgca
     2161 gccctgctgc ccaactttac ccgtggcaat gcccgcgcag acgatctggt acgcaataac
     2221 ggctatgccg ccaacgccat ccagctgcat caggatcata tcgtcgggtc ttttttccgg
     2281 ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt
     2341 tcccgcgagg ttgaagcggc atggaaagaa tttgccgagg atgactgctg ctgcattgac
     2401 gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt
     2461 aacggagaac tgttcgttca ggccacctgg gataccagtt cgtcgcggct tttccggaca
     2521 cagttccgga tggtcagccc gaagcgcatc agcaatccga acaataccgg cgacagccgg
     2581 aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc
     2641 gaggacgggt atcctggctg gatgccgcag aaatggacat ggataccccg tgagttaccc
     2701 ggcgggcgcg cctcgttcat tcacgtcttt gaacccgtgg aggacgggca gacccgcggt
     2761 gcaaatgtgt tttacagcgt gatggagcag atgaagatgc tcgacacgct gcagaacacg
     2821 cagctgcaga gcgccattgt gaaggcgatg tatgccgcca ccattgaaag tgagctggat
     2881 acgcagtcag cgatggattt tattcttggc gcgaacagta atgagcagcg ggacaagctg
     2941 gcgggctgga ttggtgaaat tgccgcgtat tacgccgccg cgccggtccg gctgggaggc
     3001 gcaaaagtgc cgcacctgat gccgggtgac tctctgaacc tgcagacggc tcaggacacg
     3061 gataacggct actccgtgtt tgagcagtca ctgctgcggt atatcgctgc cggactgggt
     3121 gtctcgtatg agcagctttc ccggaattac gcccagatga gctactccac ggcacgggcc
     3181 agtgcgaacg agtcgtgggc gtactttatg gggcggcgaa aattcgtcgc atcccgtcag
     3241 gcgagccaga tgtttctgtg ctggctggaa gaggccatcg ttcgccgcgt ggt
//
LOCUS       DAZGJW010000294         3250 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZGJW010000294 DAZGJW010000000
VERSION     DAZGJW010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.297"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..103
                     /locus_tag="ACHKIK_005108"
                     /pseudo
     CDS             1..103
                     /locus_tag="ACHKIK_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2737 family protein"
     gene            100..207
                     /locus_tag="ACHKIK_005109"
                     /pseudo
     CDS             100..207
                     /locus_tag="ACHKIK_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            684..1352
                     /locus_tag="ACHKIK_005110"
     CDS             684..1352
                     /locus_tag="ACHKIK_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO9086072.1"
                     /translation="MSLIDYQALRAKAEKATCGVWSLEYGESRFDCDDALIHREVVGY
                     LPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLALLDELERNQQYIKRRDQE
                     NEDIALTVGKLRVELEGKDKLIAELGKQCAEWERKALSNFEECAAMAERIEEMSKQSC
                     EARERDLFESWVMHSICISKSTLEGLRTETGYRNATLSGTDFNRMWKQWKSIRATGIR
                     IKGE"
     gene            1354..1542
                     /locus_tag="ACHKIK_005111"
     CDS             1354..1542
                     /locus_tag="ACHKIK_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086073.1"
                     /translation="MSLTVRQLISKLRKMPPEAVVVWQGYDQSEGEYNDFVGHVADVT
                     DENAPSFDPEVRVVALRG"
     gene            1546..2163
                     /locus_tag="ACHKIK_005112"
     CDS             1546..2163
                     /locus_tag="ACHKIK_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086074.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AEPIGEVSEKRLGLVMDGTVDLGGKSTYRIIKGEKAMKLLPLGTKFYTAPPAPIALEA
                     IENAIEYIRSIAFHIDEDDYHGKHIAYFMRQALAWLEGHSCSDDSQGKSDNSPLPRYQ
                     VIELTMLVKQLVSQLKKAKPDCKLPDRAMDYLLRNGLVSAEDVLR"
     gene            2160..2747
                     /locus_tag="ACHKIK_005113"
     CDS             2160..2747
                     /locus_tag="ACHKIK_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO9086075.1"
                     /translation="MTISTKKPLYAEVHNVPDDYEFTDEELNRIIAGDMFTPRQDAIM
                     AREIQKLRAAMLHGAEPVSQTYKSQHTQFEQVADLYEMQFDDGRTCAFHTDAQKAAQW
                     LQACDGNRVQEYVKLERLRNALSGNSPVTPDGWICCSERMPDKLIPVMVMYEDGEMWS
                     AMWNGNRWDDGTEYPDPHSVTHWREMPAAPQQEVK"
     gene            2747..3025
                     /locus_tag="ACHKIK_005114"
     CDS             2747..3025
                     /locus_tag="ACHKIK_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO9086076.1"
                     /translation="MDSFAKYTIIDWIAFLQVLLIWFYMAYRSGQWIVSVACSKGWRW
                     WNRKNKKALALDSFYEAFNLNSLQPGYVIVVTTQSGMTIQIHKPKEEK"
     gene            3183..>3250
                     /locus_tag="ACHKIK_005115"
     CDS             3183..>3250
                     /locus_tag="ACHKIK_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086077.1"
                     /translation="MACSTFNPLTLQKYQPDPEDLCS"
BASE COUNT          942 a          661 c          872 g          775 t
ORIGIN      
        1 aaagccaatg ccatcgagag agtaattgct taagagaaag agtttcggtt ctgttaatga
       61 caacaaatat ctgaatgcta tgtggcggag tgggaaaaaa tgaaacaaat gtcactaatt
      121 gagatggatg gttttctgaa aggtaaatgc atcccacgag atctaaaggt taacgaaaca
      181 aacgctgaat atcttgtccg taagttcggt gaacttgaat caaaactgga aacggcgttg
      241 cgggagtgtc gttctgctgg aatcacgatt gataaccttg aggccaagtg cgcggcgctg
      301 gcagcggaga gtgcggggat gaagaagttc tgcaaagacg ctgcattcga tgccgattac
      361 gaagcagagc taggtatgga gcgtggttta ttcagtgatg cgcttaacga aatcaaaacc
      421 ccagccaccg atgctttcct ggctgaagta cgtgcggaag cacgcaacga gggtattaac
      481 tatgccgcaa gccgtcttgc tgctgctttc aaccacggat ttatcaataa gtctttacgt
      541 gaagttttcg atgttacgcg catgattctg tcagcgaaag aagagttggc taatgaaccg
      601 catccgattg atggcctgtc tggtgaatat gcggagaaat cccttgaaga atgggcggaa
      661 cagattcgca aaggaggcaa ccagtgagcc tgattgacta tcaggcactg cgtgccaagg
      721 cagaaaaagc aacgtgtggt gtatggtcgc tcgaatatgg agagagccga tttgattgtg
      781 atgatgcgtt aattcatcgt gaagttgttg gatatcttcc catttgcaga attgaaggag
      841 cacatccaga aagcggtttc gatgaagatt tccaaatgga acagcaggcc aatgctgaat
      901 tcatcgccgc agccaatccg gctactgtgc tggcattact ggatgaactg gaaagaaacc
      961 agcaatacat caaacgccgc gatcaggaga acgaggatat tgcgctaacg gtagggaaac
     1021 tgcgtgttga gctggaaggc aaagacaagc tgattgcaga gcttggaaaa caatgcgccg
     1081 aatgggagcg aaaagcatta agcaactttg aagagtgtgc tgcgatggct gaacgtatcg
     1141 aagagatgag taagcaaagt tgcgaagccc gggagcgtga tttgtttgaa tcatgggtaa
     1201 tgcattcaat ttgtatctcc aaatcgacgc ttgaaggatt gcgtaccgaa actggatacc
     1261 gtaacgcaac cttatcaggc acagacttca accgaatgtg gaaacaatgg aaatctatcc
     1321 gcgctactgg cattcgcatc aaaggagagt gagatgtcgc tgacggttag gcagttaatc
     1381 agcaagctca gaaaaatgcc tcctgaggct gtcgtggtct ggcagggcta tgaccaatca
     1441 gagggtgagt ataacgactt tgtaggtcat gttgctgatg ttacagatga gaatgcacca
     1501 tcttttgacc cagaagtgcg cgttgtggcg ctaagaggat aacccatgac cactattacc
     1561 aaagagcgac tactgacaat caagcagtgg cgcgaaacat acggacctgg tagcaacgtt
     1621 gtactgccag cagaagaagc ggaagaactg gcacgaattg ctctggcatc gctggaagca
     1681 gagcctattg gtgaggtttc agagaagcga ctcggccttg ttatggatgg aacggtagac
     1741 cttggcggga aatcaactta tcgcatcatt aagggagaaa aagcgatgaa gttgttgccg
     1801 ctggggacga agttttatac cgcaccgcca gcgccgatag cgttggaggc cattgaaaat
     1861 gcaatagaat acatccgcag tatcgctttt cacatcgatg aagacgatta ccacggcaaa
     1921 catattgcgt atttcatgcg acaagcattg gcctggctgg aagggcattc atgcagtgat
     1981 gacagtcagg gcaaatccga taattcacca ttgccgcgct accaggtgat cgaattaaca
     2041 atgctggtta aacaattggt cagccaactg aaaaaagcaa aacctgattg caaattacct
     2101 gatagggcga tggattatct tttgcgaaac ggactggtaa gtgcggagga tgttttacga
     2161 tgaccatttc tacaaaaaag cctctttatg ctgaggttca caatgttcct gatgattatg
     2221 agttcactga cgaggaacta aacagaatta ttgcaggtga tatgttcact cctcgtcagg
     2281 acgcaataat ggcacgggag atacagaaac tccgcgccgc catgcttcat ggtgccgaac
     2341 ctgtaagcca aacttacaag tcacaacata cgcagtttga acaagttgct gacctctacg
     2401 aaatgcaatt tgatgacggt cgcacttgtg cctttcacac tgatgcgcaa aaggctgcgc
     2461 aatggcttca ggcatgcgac ggaaacaggg ttcaggaata cgtgaagctg gagcgattgc
     2521 gtaatgcgct atcgggcaac tctccggtaa ctccggatgg ttggatatgc tgtagtgagc
     2581 gaatgccgga taagttaatt ccggtaatgg tcatgtatga agacggtgag atgtggtctg
     2641 caatgtggaa tggcaatcgc tgggatgatg gcaccgaata tccggatccg cactcagtta
     2701 cgcactggcg tgaaatgcca gcagcaccgc agcaggaggt gaagtgatgg actccttcgc
     2761 gaaatatacg attattgact ggatagcatt ccttcaggtt ttgctcatct ggttttatat
     2821 ggcttacagg agtgggcagt ggattgtcag tgtagcctgt agcaagggat ggcgttggtg
     2881 gaaccgaaag aataaaaaag cgctggcctt ggattcgttt tacgaagcat tcaatcttaa
     2941 cagtcttcag cctggttatg tcattgtagt caccactcaa agcggcatga ccattcagat
     3001 tcataaacca aaagaggaaa aatgatgtgg cctatatgtg ttaattgcgg acggatgtgc
     3061 ctatctggat ggtgccgaaa gtgcgacaaa tgcacgaaga aaagacaata acaatcctcg
     3121 cactgacggg gatttctttt atctgaactc gctacggcga gttttgtttt atggagatga
     3181 ttatggcctg ttcaacattc aaccctttaa cgttacagaa ataccagcca gaccctgaag
     3241 atttatgctc
//
LOCUS       DAZGJW010000295         3239 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZGJW010000295 DAZGJW010000000
VERSION     DAZGJW010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.298"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..349
                     /locus_tag="ACHKIK_005116"
     CDS             <1..349
                     /locus_tag="ACHKIK_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HFO9086078.1"
                     /translation="QLALLGKTLGNKGIGLEGGGIFTVGDECHAHFLVEQGKEAKIAL
                     EQAGLLVLAIRTPLIRKLKHEKPGELGEIARVLAENNINILVQYSDHANQLILITDND
                     SMAASVTLPWAIK"
     gene            357..1043
                     /locus_tag="ACHKIK_005117"
     CDS             357..1043
                     /locus_tag="ACHKIK_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFO9086079.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1021..1644)
                     /gene="tetR(B)"
                     /locus_tag="ACHKIK_005118"
     CDS             complement(1021..1644)
                     /gene="tetR(B)"
                     /locus_tag="ACHKIK_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFO9086080.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1726..2931
                     /gene="tet(B)"
                     /locus_tag="ACHKIK_005119"
     CDS             1726..2931
                     /gene="tet(B)"
                     /locus_tag="ACHKIK_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFO9086081.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3044..>3239)
                     /locus_tag="ACHKIK_005120"
     CDS             complement(3044..>3239)
                     /locus_tag="ACHKIK_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFO9086082.1"
                     /translation="VNAIVSKLIQENQLKAYDDDVLKNLLNGWFMHIAIHAKNLKELA
                     DKKGQFIAIYRGFLLSLKDK"
BASE COUNT          869 a          611 c          715 g         1044 t
ORIGIN      
        1 acaattagca ttactaggaa aaacattagg taataaaggt attggattgg aagggggagg
       61 gatatttacg gttggtgatg aatgccatgc tcattttctt gttgaacaag gaaaggaagc
      121 taaaatagcg ctagagcaag ctggactgtt agtacttgcg atccggacac cattaattcg
      181 taagttaaaa catgaaaaac cgggggaact tggcgaaata gcacgagtat tggcggagaa
      241 taacattaat attttagtgc aatacagtga ccatgctaac caactgatat taataacgga
      301 caatgatagt atggctgcat ctgttacgct cccttgggca ataaagtgaa cttgcgatgg
      361 ctaatttaat acgaaaagag gttacctttg agtcctcaat agccgcgata ggggcggcta
      421 tgtctgacat ttcacgagtt aaaatactca gtgctttgat ggatgggcga gcttggacgg
      481 ccactgagct aagttctgtg gcgaatatat cagcttcaac ggcgagcagt catttatcta
      541 aattattaga ttgccagcta atcacagtag tagctcaagg caagcatcgt tattttcggc
      601 tagcaggaaa agatattgct gaattgatgg aaagtatgat ggggatctcc ttaaaccatg
      661 gcgtacatgc caaagtttcc acgccagtgc atttacgaaa agcacgtact tgctatgatc
      721 atttagctgg cgaagttgcc gttaagatct atgattccct ttgtcaacag caatggatca
      781 ctgaaaatgg ttcaatgatc acattaagtg gtattcaata ttttcatgaa atgggaattg
      841 acgttccttc caaacattca cgtaaaatct gttgtgcgtg tttagattgg agtgaacgcc
      901 gtttccattt aggtgggtac gttggagccg cattattttc gctttatgaa tctaaagggt
      961 ggttaactcg acatcttggt taccgtgaag ttaccatcac ggaaaaaggt tatgctgctt
     1021 ttaagaccca ctttcacatt taagttgttt ttctaatccg catatgatca attcaaggcc
     1081 gaataagaag gctggctctg caccttggtg atcaaataat tcgatagctt gtcgtaataa
     1141 tggcggcata ctatcagtag taggtgtttc cctttcttct ttagcgactt gatgctcttg
     1201 atcttccaat acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat ataatgcatt
     1261 ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga ttttcgagag tttcatactg
     1321 tttttctgta ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc
     1381 acatctaaaa cttttagcgt tattacgtaa aaaatcttgc cagctttccc cttctaaagg
     1441 gcaaaagtga gtatggtgcc tatctaacat ctcaatggct aaggcgtcga gcaaagcccg
     1501 cttatttttt acatgccaat acaatgtagg ctgctctaca cctagcttct gggcgagttt
     1561 acgggttgtt aaaccttcga ttccgacctc attaagcagc tctaatgcgc tgttaatcac
     1621 tttactttta tctaatctag acatcattaa ttcctaattt ttgttgacac tctatcattg
     1681 atagagttat tttaccactc cctatcagtg atagagaaaa gtgaaatgaa tagttcgaca
     1741 aagatcgcat tggtaattac gttactcgat gccatgggga ttggccttat catgccagtc
     1801 ttgccaacgt tattacgtga atttattgct tcggaagata tcgctaacca ctttggcgta
     1861 ttgcttgcac tttatgcgtt aatgcaggtt atctttgctc cttggcttgg aaaaatgtct
     1921 gaccgatttg gtcggcgccc agtgctgttg ttgtcattaa taggcgcatc gctggattac
     1981 ttattgctgg ctttttcaag tgcgctttgg atgctgtatt taggccgttt gctttcaggg
     2041 atcacaggag ctactggggc tgtcgcggca tcggtcattg ccgataccac ctcagcttct
     2101 caacgcgtga agtggttcgg ttggttaggg gcaagttttg ggcttggttt aatagcgggg
     2161 cctattattg gtggttttgc aggagagatt tcaccgcata gtcccttttt tatcgctgcg
     2221 ttgctaaata ttgtcacttt ccttgtggtt atgttttggt tccgtgaaac caaaaataca
     2281 cgtgataata cagataccga agtaggggtt gagacgcaat cgaattcggt atacatcact
     2341 ttatttaaaa cgatgcccat tttgttgatt atttattttt cagcgcaatt gataggccaa
     2401 attcccgcaa cggtgtgggt gctatttacc gaaaatcgtt ttggatggaa tagcatgatg
     2461 gttggctttt cattagcggg tcttggtctt ttacactcag tattccaagc ctttgtggca
     2521 ggaagaatag ccactaaatg gggcgaaaaa acggcagtac tgctcggatt tattgcagat
     2581 agtagtgcat ttgccttttt agcgtttata tctgaaggtt ggttagtttt ccctgtttta
     2641 attttattgg ctggtggtgg gatcgcttta cctgcattac agggagtgat gtctatccaa
     2701 acaaagagtc atcagcaagg tgctttacag ggattattgg tgagccttac caatgcaacc
     2761 ggtgttattg gcccattact gtttgctgtt atttataatc attcactacc aatttgggat
     2821 ggctggattt ggattattgg tttagcgttt tactgtatta ttatcctgct atcgatgacc
     2881 ttcatgttaa cccctcaagc tcaggggagt aaacaggaga caagtgctta gttatttcgt
     2941 caccaaatga tgttattccg cgaaatataa tgaccctctt gataacccaa gagggcattt
     3001 tttacgataa agaagattta gcttcaaata aaacctatct attttattta tctttcaagc
     3061 tcaataaaaa gccgcggtaa atagcaataa attggccttt tttatcggca agctctttta
     3121 ggtttttcgc atgtattgcg atatgcataa accagccatt gagtaagttt ttaagcacat
     3181 catcatcata agctttaagt tggttctctt ggatcaattt gctgacaatg gcgtttacc
//
LOCUS       DAZGJW010000296         3238 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZGJW010000296 DAZGJW010000000
VERSION     DAZGJW010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.299"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(23..1015)
                     /gene="rfaC"
                     /locus_tag="ACHKIK_005121"
     CDS             complement(23..1015)
                     /gene="rfaC"
                     /locus_tag="ACHKIK_005121"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO9086083.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            complement(1019..2065)
                     /gene="rfaF"
                     /locus_tag="ACHKIK_005122"
     CDS             complement(1019..2065)
                     /gene="rfaF"
                     /locus_tag="ACHKIK_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO9086084.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(2075..3007)
                     /gene="rfaD"
                     /locus_tag="ACHKIK_005123"
     CDS             complement(2075..3007)
                     /gene="rfaD"
                     /locus_tag="ACHKIK_005123"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO9086085.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
BASE COUNT          775 a          852 c          794 g          817 t
ORIGIN      
        1 agagagaaaa ataatgttga ggtcatctta tctccgatgt caacttattg gttattattt
       61 caagatttaa attttgaaaa acggtttgag ctggcaaatc tgcaagagac atacttgtag
      121 aacgacactc tacttgattc ttcccatacc caccaattaa tcccggatcg gttggtccat
      181 aaaccgtgat attgggtcta tccagtgccg ccgttaaatg gcttaacccc gtatccaccg
      241 acactacaaa tttagccccg gccagcacgc gggcaacgcc ttccagactc atcttcggca
      301 atacttcaac ataagcaaat ccttccgcca gtcgtttcgc ccgttcttcc tcatgcggcg
      361 cgccccacgg aagtttaatc cgtattcctg aatcagccag taaaccaatc aattctcgcc
      421 agtgttcttc cggccagtgt ttatcatcac gggtcgtcgc atgaagaaat acggcatatt
      481 cgccagcatc tgtaggcagg ttcgtcagaa aatgctgtgc gatagcataa tcgccctggg
      541 tttgcggttt gctatagccc aaacttttgg caaacagttc gcgggtgcgt tctacggcgt
      601 gctgctgttt tgcaatatga tgcttacaat tgtaaaacag gctggctaaa ggttcgcgag
      661 cggtttgcca gtccaatcca tgctttacgc catgcgccag acgtgtcacc agtgccgcgc
      721 tttttaccag cccctgagcg tcgataactg cgtcatagtt ctctgcttgt agcgcttcac
      781 gaaacgcttt gcgttcagct tttatggggg ccgagaacca ggctttacgc cagcgacgta
      841 ttgccacagg aataactcgc tcaacggcag cgtgccagga aggaatctgt gcgaaccctt
      901 cttccaccac ccagtcaaac ttaatccctg ggattgcctg ctgggcatca gtgagtgcgg
      961 gcaacgtatg gagaacatcg cccatcgacg atgttttaac gatcaaaacc cgcatccgtc
     1021 aggcttcctc ttgtaacaat agcgcgttga gttcttccag tacgcgctgg ggagtaatgt
     1081 cgatcaagct ctggtgataa ccctccgcag cgtcaccttt acgcactttg tgatagccgg
     1141 taatcagacg gatcacgcgc gctttatggg atagcggcgg tgtgaagtcc gggctacttg
     1201 gaccatacag ggcaaccagc ggacgattga gcgccgccgc aacgtgcatc aggccagaat
     1261 cgttagtgac aatggcttta caggctgcaa tcaggataac cgcttgatca agctgtgttt
     1321 cccccgccag gttccgacac catgcctgct gctcggtatt taaagcggca agaatctcat
     1381 tgcctgcttc atgatctttc gccgagccaa acagaaccac ctgataacct tcatcaatca
     1441 gctgctttgc cagctccgca tagtggtagt gtggccagcg ttttgccgga ccaaactccg
     1501 cacccgggca aaaaccaatc atcggacgtt ctgatgaaag cgaaaattga ttacaggtat
     1561 atgatttttc accttcgctc acctgcaact gcggccataa caatggctgc ggcagatctt
     1621 gtgctgtgcg cataatgcct ttgtcatagg ccagcgctat atagcgttcc accattagcg
     1681 gccaggcttc tttatcgagc acgcgtacat cgttgagtaa accgtagcgc atctcgccgc
     1741 gccagccggt gcgatgagga atacccgcga agaaaggcac taatgcggat ttgaaggagt
     1801 taggtaagac gtaggcgcgg tcgtagcgct tttcacgcag gctatgaccc agtttgcggc
     1861 gttcgccgat ttccagcgct ccgtgaccga gaggcatagg gatagcttcg ttaacttccg
     1921 gcatccgcga taataatgga cggcaccatg ccggtgccat cacatcgatt atcgcctggg
     1981 gatagcgcgc ctggagcgtg cgatagagac tttgcgacat catcatgtcg ccaacccaag
     2041 acgggccgat caccagtatt ttcatgcaga gctcttatgc gtcgcgattc agccaggcca
     2101 tgtattccgt tacaccttca gcaacggttt tgaacggttt gtcgtaaccc gccgcgcgca
     2161 gatttgtcag atctgcctga gtgaacgcct ggtagcggcc tttcagctta tccgggaacg
     2221 gaatgtattc gatctggcct ttcttgtgat aagccagcgt agcatcagct acagcctgga
     2281 aggattccgc acgaccggtg ccgaggttga agatgccgga aacgccattt tccaggaacc
     2341 acaggtttac atctgccaca tcgccaacat agacgaagtc gcgtttaaag ttctcgctac
     2401 cttcgaacag cttcggtgat tcaccgttat taagctgggt gttgagatgg aaagcgacgc
     2461 tcgccatgct gcctttatgg ccttcacgcg gtccataaac gttgaaatag cggaagccaa
     2521 caatctgcga gttcgcttcc ggcaggattt ggcgaacata ttcatcaaac aggaattttg
     2581 agtaaccgta gacgttcaac ggtttttcgt actcgcggga ttcaataaag tcggaggtgc
     2641 gtccgccgta ggtggctgcg gaagaagcgt acaggaacgg gatttcacgc tccaggcagt
     2701 agtgcagcag ctccttggag tattgatagt tgttatccat catatacttg ccgtcccact
     2761 cggtggtgga agagcacgcg ccttcgtgga aaatcgcttc gacatcgccg aactcttcgc
     2821 cagccataat ctggatcagg aagtcttcct tatccatata gtctgcgata ttcagatcca
     2881 ccaggttcac aaacttggtg ccgtctttca ggttgtccac caccagaata tcggtgatgc
     2941 ctttatcatt cagggcttta acgatgttgc tgccgataaa gcccgcgccg ccggtaacga
     3001 tgatcataac tgtaaccttc gaattatgga gtcagagaca atctcagaca cgaatgtctg
     3061 taatcatacc atcacatccg aatcccttca gccctttaca tagaggtgca aggatgctaa
     3121 ttgcaacaaa ctagtttggg ttttagctag tccttcatgg cggatgttgg ttttgcatgt
     3181 gaatagtgct ttagtatcaa tattccgatg tatgataaat aacggaagca tttgaatg
//
LOCUS       DAZGJW010000297         3220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZGJW010000297 DAZGJW010000000
VERSION     DAZGJW010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.300"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..454)
                     /locus_tag="ACHKIK_005124"
     CDS             complement(<1..454)
                     /locus_tag="ACHKIK_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO9086086.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAA"
     gene            complement(474..935)
                     /locus_tag="ACHKIK_005125"
     CDS             complement(474..935)
                     /locus_tag="ACHKIK_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO9086087.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(963..2864)
                     /locus_tag="ACHKIK_005126"
     CDS             complement(963..2864)
                     /locus_tag="ACHKIK_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO9086088.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDELQRQVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVRF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT          676 a          913 c          822 g          809 t
ORIGIN      
        1 ttgcagcggg ctttccgtta atgtttacat tttcagaacc ggtattgatg gcgccgtccg
       61 tgtcacaaaa gctgtttatc catgattcaa gctggttctg tttctcctcc acataatcac
      121 tcgccttata gctggcatac atcagcgcag gcaccagaac cagcgcataa gggccggtgg
      181 cgcaggcggc cataatcagg caaccttctg cgatggtcac tatcgccccc agcacggccc
      241 ctgccagtgc ccccagaaaa cttttatgct gaatttcatc gcccagcctg gcggcaggtg
      301 ggccggactt ctttgctgcc agcatctgtg caatggcttt gtcgttggct gcccgtttca
      361 tctggctggc gaccatcgcc tgctgtgaca gcattcgcat tgccagcgta ccgcctgcgg
      421 tcgctccctg tggcccgcct ggtccttcac tcatgtgtta tccctcgttt atgtcagcgc
      481 ggctggtcct tttggatgtc attctccggt ggcaacgggg tgaagctctt tatcacctcc
      541 agcagcatcg tcttttgact gttcttcagg gataaggagg tgctgaaagt gaaggtcagc
      601 agcgtatctc cacgccgctg gaatacggct atctggctaa tccgcccctg attcccctca
      661 tcttccggac cgttccagga ataatccagc agccaggccg gggcctctgc cagagtgatt
      721 tcccggcagg catgttcggt gtacccatca agtttgccct ggtacagcgc cagcgtttcc
      781 tggtacagct ctggtgtcag ggttccctgt ggaacggctt cgcgggtaat gaccaggttg
      841 atgccactgt tatctgtgga caccaggacc tgcattgact gatcctgcca ttgagacggc
      901 agttgaatgt ggccttcatt aaaagtgaac tgcataaaat actccctgat aatctgagtg
      961 aatcagttga tgaaaacaga accgcccacg atggtgatgc catccttcga cagcctgatt
     1021 accgtcgatt ccccgacctg tagctgaatg ccatccggcg tcagcgcaat tttgctttcc
     1081 tccacagcca gcaaaatttc cttaatcgcc gtataagcct gtataccgtc gccttcgcca
     1141 ccattgttta cagcgtcaac atgcaccgaa ccggcaatcg tgcctctgtc ctgaaagaca
     1201 tcctcagata aagacccttt cagtactccg gtgatcttgt ggcctttttc caccgtaaga
     1261 aggcggttac cttcctggat cgtgaccgtc tggtcatttt ttacggtttc gcggtgatcg
     1321 tgttttacgg tggtggtacg gtcgttgagc acttcggtat ccatgttctt ctgcgcgtgg
     1381 atgtagacct gttctctccc tgtcgcatca tcaaacttca gctcattaaa tccgctgccc
     1441 atgtacgttt tggagcggat ggtcatctgc gtctttgttc ccggcaggct gccgggggtg
     1501 cggttttcct ggtggtaggt gcgccccata atgatcggct ggtccggatc gccgttgagg
     1561 aagtctacaa tcacctcctg acccacacgc ggtatcgcca ggtggccaaa accggtgcct
     1621 gcccacgcct gtgccacacg gatccagcac gaactgtctt ggtctgccgg gttataacgg
     1681 tcccagttga acctcacccg cacgcgacca tgttcgtcgc agaagatttc ctcgcctgcc
     1741 ggtcctgtca cgacagcgct ctgcgggccg tcgaccagcg gtttcagcaa cggctgtggt
     1801 cgccatgttc tgtctgccgg gatcaccgca aaatggttct ccagcgccgt ccccgctccc
     1861 tgccgtcctg gcaccgcctg tggctgttcg ccgtgcagtt cacttgccac cacctgccat
     1921 tcccggttta ggttcgcctg cggatgcccc gtcagcacaa ttcgccgtcc cggccatatc
     1981 tccggcgagc ggctcattcc ccgcgcggtt tctgcgttgt ttcgccagcc gtccatctgc
     2041 cagcgggcaa agttctgccc gtgggcgctc ttgaaacgtc cggggtagtc atacacttca
     2101 tactgcgtgc gctggtagtc ctggtgctgg ccttcctgtt caaaacgtcc ggcccagccg
     2161 gggcgtttaa aggtgtagtc tttggtcacc acggaagaag ggcggatttg tgcgctgtag
     2221 cggaactggc tgatgcagag ggtgctcacc tcggtacggg tgttcgggtt ccatgggatt
     2281 tcaaaagatt cgggcagatg gcggactgtg tcgcacagca ccaggctctg gtcggtactt
     2341 ttgtaagcat gctcctcata aaagaagatg ccttcctccg ccgccatccg gcacagaaaa
     2401 tcgtaatcag tctcaccgta ctggacacaa aactcacggg aaggatgcgg ctcgctgaac
     2461 agcggactcc attcggtcac cccgttttcc tgcaacatcg tgccgaggat gcttttgatg
     2521 tcctcgttct ggaagatacg gaagttctgg cgcagaccgg cacgccacag cggcgggtgc
     2581 accttcatgc tgtacagcat ctggtttttg tcgttctccc cgagttcaaa ccaggtcacc
     2641 acgcctttca cctggcgctg taattcatcg ccctgccata tcgtcaggta ggccattttg
     2701 tccagcacct gcgcaaattc aagggagaga aactgctggc tgaccaggga gagatcgagg
     2761 gaaaagagcg aagagagtga ctggttcaga tgaaaggaaa ccaccgcaaa agcgtccggc
     2821 ggcaggccgt ccacttccag tgtgaaacgt aatccggttg acatagcaac ctccggtaaa
     2881 atgaataatg atccgtaccg tacaactgcg gataatgtga taagcgggac gacttcagac
     2941 gttattaggg aaggaacaga aaatgctatg gttatgtgag aattttgttt aaatattact
     3001 ttacagaaac gggtgtctgt tcagtcaaaa aaataatcac aagctcaacc tcttgtttct
     3061 tggcgttatt caggacaggt acgcatatca caacgagtaa caaaaacgca aagttaaacc
     3121 aatattcata atatattttt attcacagca ataaataatc caattaacat tattaaaatg
     3181 aatgattgca taagaaatat atgctaccgc tccaccctga
//
LOCUS       DAZGJW010000298         3212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZGJW010000298 DAZGJW010000000
VERSION     DAZGJW010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.301"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            132..1814
                     /gene="ydeN"
                     /locus_tag="ACHKIK_005127"
     CDS             132..1814
                     /gene="ydeN"
                     /locus_tag="ACHKIK_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO9086089.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1866..3023
                     /gene="ydeM"
                     /locus_tag="ACHKIK_005128"
     CDS             1866..3023
                     /gene="ydeM"
                     /locus_tag="ACHKIK_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO9086090.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKNLFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2989..3111)
                     /locus_tag="ACHKIK_005129"
     CDS             complement(2989..3111)
                     /locus_tag="ACHKIK_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086091.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKGRVINYSPFVLQHP"
BASE COUNT          976 a          694 c          641 g          901 t
ORIGIN      
        1 ctttatggac tgttatttcc ctttttttag ctaaatctgc tatctcttta tgtgactaac
       61 ttcacttaca tccacttatt tctcttcgta aaattacttc ggaattaagt acaataagaa
      121 gaggaacatt tatgaagtct gcattaaaga aaagtgtcgt aagtacctcg atatctttga
      181 tactggcatc tggtatggct gcatttgctg ctcatgcggc agatgatgta aagctgaaag
      241 caaccaaaac aaacgttgct ttctcagact ttacgccgac tgaatacagt accaaaggaa
      301 agccaaatat tatcgtacta accatggatg atcttggtta tggacaactt ccttttgata
      361 agggatcttt tgacccaaaa acaatggaaa atcgtgaagt tgtcgatacc tacaaaatag
      421 ggatagataa agccattgaa gctgcacaaa aatcaacgcc gacgctcctt tcattaatgg
      481 atgaaggcgt acggtttact aacggctatg tggcacacgg tgtttccggc ccctcccgtg
      541 ccgcaataat gaccggtcga gctcccgccc gctttggtgt ctattccaat accgatgctc
      601 aggatggtat tccgctaaca gaaactttct tgcctgaatt attccagaat catggttatt
      661 acactgcagc agtaggtaaa tggcacttgt caaaaatcag taatgtgccg gtaccggaag
      721 ataaacaaac gcgtgactat catgacaact tcaccacatt ttctgcggaa gaatggcaac
      781 ctcaaaaccg tggctttgat tactttatgg gattccacgc tgcaggaacg gcatattaca
      841 actccccttc actgttcaaa aatcgtgaac gtgtccccgc aaaaggttat atcagcgatc
      901 agttaaccga tgaggcaatt ggcgttgttg atcgtgccaa aacacttgac cagcctttta
      961 tgctttacct ggcttataat gctccgcacc tgccaaatga taatcctgca ccggatcaat
     1021 atcagaagca atttaatacc ggtagtcaaa cagcagataa ctactacgct tccgtttatt
     1081 ctgttgatca gggtgtaaaa cgcattctcg aacaactgaa gaaaaacgga cagtatgaca
     1141 atacaattat tctctttacc tccgataatg gtgcggttat cgatggtcct ctgccgctga
     1201 acggggcgca aaaaggctat aagagtcaga cctatcctgg cggtactcac accccaatgt
     1261 ttatgtggtg gaaaggaaaa cttcaacccg gtaattatga caagctgatt tccgcaatgg
     1321 atttctaccc gacagctctt gatgcagccg atatcagcat tccaaaagac cttaagctgg
     1381 atggcgtttc cttgctgccc tggttgcaag ataagaaaca aggcgagcca cataaaaatc
     1441 tgacctggat aacctcttat tctcactggt ttgacgagga aaatattcca ttctgggata
     1501 attaccacaa atttgttcgc catcagtcag acgattaccc gcataacccc aacactgagg
     1561 acttaagcca attctcttat acggtgagaa ataacgatta ttcgcttgtc tatacagtag
     1621 aaaacaatca gttaggtctc tacaaactga cggatctaca gcaaaaagat aaccttgccg
     1681 ccgccaatcc gcaggtcgtt aaagagatgc aaggcgtggt aagagagttt atcgacagca
     1741 gccagccacc gcttagcgaa gtaaatcagg agaagtttaa caatatcaag aaagcactaa
     1801 gcgaagcgaa ataactaaac cttcatgcgg cggaattttc cgccgcctta ttgagcgaga
     1861 tagcgatgca cgttacagcc aagccctcca gttttcaatg taatctcaaa tgtgattact
     1921 gtttttacct tgaaaaagag tcgcagttta ctcatgaaaa atggatggat gacagcactc
     1981 tgaaagagtt catcaaacaa tatatcgcag cgtctggcaa tcaggtctat tttacctggc
     2041 aaggcggtga acccactctg gctggcctgg attttttccg taaagttatt cactatcaac
     2101 aacgctatgc aggccaaaaa cgtattttta atgcattaca aacgaatggc attttattga
     2161 ataatgaatg gtgtgctttt ctcaaagaac atgagtttct ggttggtatc tcgatcgatg
     2221 gcccccagga gctacatgac cgttacagac gcagtaattc aggtaacggt acttttgcaa
     2281 aagtgatagc agccatcgag cgtctgaaat catatcaagt tgagtttaat acgttaaccg
     2341 tcattaataa cgttaatgtc cattaccctc ttgaggttta tcatttttta aaatctatcg
     2401 gcagtaaaca tatgcaattt atcgaattgc tcgaaaccgg gacgccgaat attgatttca
     2461 gtggtcatag tgagaacaca ttccgtatca ttgatttttc tgtgcctccc acggcttatg
     2521 gcaagtttat gtcaaccatt tttatgcaat gggttaaaaa cgatgtgggt gaaattttca
     2581 ttcgtcagtt tgaaagcttt gtcagccgtt ttttggggaa tgggcatacc agttgtattt
     2641 tccaggagtc ctgcaaggat aatctggttg ttgaaagtaa tggagacatt tacgaatgcg
     2701 accattttgt ctatccacag tacaaaattg gaaacattaa taaatctgaa ctcaaaacga
     2761 tgaacagtgt acaactgaca gcgcaaaaaa aacggatttc agcgaaatgt cagcaatgtg
     2821 catataaacc tatctgcaat ggcggttgtc ctaagcatcg tattactaaa gtaaacaatg
     2881 agactgtttc ttatttttgc gaaggttata aaaacctttt ttcaaccatg gtaccttata
     2941 tgaacgccat ggtggagtta gctaagaaca gagtaccgct ttatcacatt atggatgttg
     3001 caaaacaaat ggagaataat taataacacg cccttttagc tccattctta gcctccttct
     3061 tatgacctta acaaggcacc cgacataaat tcctgtcggg tgtttcgtca tcatcatctt
     3121 cccttgcgaa aatcctggct tacgtggtat gcgtcagggc attagcaata cccttgatta
     3181 tcaatagcat aaccataact aatgaaaatg aa
//
LOCUS       DAZGJW010000299         3201 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZGJW010000299 DAZGJW010000000
VERSION     DAZGJW010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.302"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>3201)
                     /locus_tag="ACHKIK_005130"
     CDS             complement(<1..>3201)
                     /locus_tag="ACHKIK_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9086092.1"
                     /translation="DNLKSTQLLSVIDAISEGPIEGPVDGLKSVLLNSTPVLDSEGNT
                     NISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITRTITSANIDRLRFTFG
                     VQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTTSQYLASVVVDNLPPR
                     PFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALVGVQVDSEQFGSQQVS
                     RNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCLWDMLTHPRYGMGKRL
                     GAADVDKWALYVIGQYCDQSVPDGFGGTELRITCNAYLTTQRKAWDVLSDFCSAMRCM
                     PVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSALKDRHNAVEVNWIDPN
                     NGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLWLIKTELLETQTVDFS
                     VGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLDREITLPSSGTTLISL
                     VDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLRQRLFRCVSIRENDD
                     GTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVLA
                     RWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQLALGRYTLTVRAVNA
                     WGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIAD
                     IRQVETTARYLGTALYWIAASINIKPGHDYYFYICSVNTVGKSAFVEAVGQPSDDASG
                     YLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSNEITQTVNKKLEDQSA
                     AIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTSDGMQSQVLLAADR
                     IAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKN
                     ADISGSVNANAGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPS
                     GTITVRVYDDQPFDRQIVIPAVAFSGAKHEREHTDI"
BASE COUNT          625 a         1002 c          828 g          746 t
ORIGIN      
        1 aaatatcagt atgctctctc tcatgtttag cgccgctgaa tgccaccgcc ggaataacaa
       61 tctgccggtc aaacggctga tcgtcataaa ccctgacggt aatggtccct gatggccacc
      121 gttccggtgc acgggagtcc cgggggaaag ctttgcccac tgttttaacg agatcgcctt
      181 caatctggtt cgcggacagt tttcccagaa cccgacagtt ctcgttaatc gtgacattgt
      241 tgagcgtccc ggcgttcgca ttcacactgc cactgatatc cgcattttta gcggtcagct
      301 ttccgtccgg tgtcagggaa aatgccggtg gatttccacc gctggtaatg gtgggggccg
      361 tcaggcgttt caggaacacg tcgttcatga atatctgatc gccctgacca acaaacatcg
      421 gttttgtgtt gccattcgca ggattaacca tcgcaatcct gtccgccgcc agcagcacct
      481 gactctgcat gccgtcagag gtgttctcaa taccggcacc aatacccgcg atataaaggc
      541 gtccgtcctg catctgctgc agcttcacag cccacatgct gttcaggtta ttatttgtat
      601 caacctgaac cttctgtatc tgctgaattg ccgcactctg gtcttccagt ttcttattga
      661 cggtctgcgt gatttcattg ctgacatccg taatggacgt cctgatttca gccaggtcag
      721 gcgcaagctg accgttatca atctgcgtcc acaactcctg agccagatgg gttttcccta
      781 tctcgccttt gaaaaaatcc agatagccgg atgcatcatc actcggctgg ccgacggcct
      841 ccacaaatgc cgatttgcca acggtgttca cactgcagat gtaaaaataa taatcatggc
      901 ccggcttaat attgatactg gcagctatcc agtacagcgc cgtgccaaga tagcgggctg
      961 tggtttcaac ctgcctgata tccgcaatcc gcttttccga gaaccagaat tcaaactgta
     1021 ccgtcggatc ataaaccgca agatgcggcg tggcggttat ctgaaaatag cccggcgtca
     1081 gctcaatccg cgacggcgct gccggtgcgg caatccggaa cgataccgac gccggatcgc
     1141 cctgctgtcc ccacgcattt accgcccgga ctgtcagcgt gtaacgcccc agcgccagtt
     1201 gcctgaagcg gtatgtggtt tccgtcgtcc gggccgtgct gaccagccgc tcactgccgt
     1261 catccgctgc cacggtcagg cgaagaagga agctaacgcc cttcaccacc ttcggcgtgt
     1321 cccagcgcgc cagcacctga tattccccgc tgtctgcggt gacttcggca gtcaggtgct
     1381 gtaccgctgg cggcgtgaca ccattcaccg tgccgctctg gtcgccgtca aagtgcgccc
     1441 cgttatccac gatggcttct ttttccggta catgctgcac ggcagtgatg gcatacgtgc
     1501 cgtcatcgtt ctcacggata ctcacgcagc ggaacaggcg ctggcgcagc gtcggcaact
     1561 tcagccccca tacgctgtat tcagcaacgc cgtcaggaac acggctcact ttcaccttca
     1621 cgccgtcggt gacggactga acctccacgc tgaccggatt gccacttccg tcaaccaggc
     1681 ttatcagcgt ggtaccggag gatggcagcg tgatttcacg gtcgagcgtc agcgtccggg
     1741 tctggctgtt caccgccagc acgcgcccgc cggtgctgat accggcatag tcatcatcgc
     1801 agatttcaat gacatcgccc ggcacatggc gaagcccttc tgcgcccacg ctgaagtcca
     1861 cggtctgcgt ttccagcagt tctgttttaa tcagccacag cccggcgcgg tgtgcctgcc
     1921 cccggctggt acagccaaaa gcatccatct tcgtgacgtt acgaccgtaa cgggcaatgg
     1981 cctgcgtatc ctccacaagc tctgtcgccg tctcccagcc gttgttcggg tcaatccagt
     2041 tcacctcaac ggcattatgg cggtccttca gggcgctgaa gctgtagcgg aacggcgcgc
     2101 catcatccgg catcaccaca ttactgcggt tataggtcca caccttatcc gacggtcggt
     2161 cctgcacgaa cgtcagcgtc tgcccgttcc ataccggcat acagcgcatc gccgagcaga
     2221 aatcactgag cacatcccac gccttgcgct gtgtggtcag gtacgcatta caggtgatgc
     2281 gcagctccgt gccgccaaag ccgtccggca ctgactggtc gcagtactgg ccgatgacat
     2341 acagcgccca tttgtccaca tccgccgcac caagacgttt ccccatgccg tagcgcgggt
     2401 gggtcagcat atcccacaga caccaggcca tgttgttgct gtatgctggt tttaacgttc
     2461 cgtcccagat accgctgtat tgtcgcgtct gcgggttata gttcgacggc acctgcagaa
     2521 tgcgcccgcg aagatgataa ttacgactca cctgctggct gccgaactgc tccgagtcca
     2581 cctgtacgcc gaccagtgcc gtgttcgggt agcactgttt cacatcgatg atttcggtgt
     2641 atgacgacca gagcgttttg ttctgcagct ggtctgtggt gctgtccggc gtcatcctgc
     2701 gcatccggat attaaacggg cgcggaggca ggttatccac caccaccgag gccagatact
     2761 gcgaggtggt tttgccctta atggtgatgt ctttttccgt cacccagcca ccgttacgct
     2821 gtatctgaac cagcaggcgg acttccgacg gattccggtc cccctttgag gtggtttcca
     2881 ccagtgcctg cacaccgaag gtaaagcgca gacggtcgat gtttgccgac gtgatggtgc
     2941 gggtgatcgg cgtgtcatat ttcacttccg tacccagcac cgtctcggag ccggaggatt
     3001 caaaaccttc cggcggtgtc tgctcctgct cacctgcccg gaacaccacc gtgacgccgg
     3061 agatgttggt attcccctca ctgtccagca ccggcgtact gttcagcagc acgcttttta
     3121 atccatccac cggaccttca atcggccctt cgctgatggc atcaatcaca ctcagcagct
     3181 gcgtggattt caggttatct t
//
LOCUS       DAZGJW010000300         3189 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZGJW010000300 DAZGJW010000000
VERSION     DAZGJW010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.303"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..850
                     /locus_tag="ACHKIK_005131"
                     /pseudo
     CDS             1..850
                     /locus_tag="ACHKIK_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
     gene            complement(890..1182)
                     /locus_tag="ACHKIK_005132"
                     /pseudo
     CDS             complement(890..1182)
                     /locus_tag="ACHKIK_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(1262..2883)
                     /locus_tag="ACHKIK_005133"
                     /pseudo
     CDS             complement(1262..2883)
                     /locus_tag="ACHKIK_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          783 a          836 c          761 g          809 t
ORIGIN      
        1 ttattgctgc gctgataata tggaccgcaa acctattgaa gatgtgatct tcgaaattaa
       61 taactttatc tcgctcggtg gtcgcaccat tgttgatgcc acagggtctg agtctatcgg
      121 acgagatgcg caggcattac gagaagtcgc attaaaaaca ggtctgaata ttgttgcttc
      181 ctccggtccc tacctggaaa aatttgaaag ccagagaatt cataaaacgg ttgatgaact
      241 ggcggcaacg attgataaag aattgaatca agggattggc gatacggata ttcgtgccgg
      301 aatgatcggt gaaataggtg tctcaccgac atttaccgaa gccgagcata acagcttgcg
      361 ggctgcctcg ctggcacaga ttaacaatcc tcatgtggcg atgaatattc acatgccggg
      421 ctggcttcgt cgcggtgatg aagtactcga cattgtgtta ggtgaaatgg gcgtctcgcc
      481 aaataaagtc tctctcgcac actcggatcc gtcaggaaaa gacgtggcgt atcagcggaa
      541 aatgcttgat aagggtgtct ggctggaatt cgacatgatt ggcctagaca ttaccttccc
      601 gaaagaggga atagcgccag gggtgcagga gactgccgat gccgtcgctc atctcattga
      661 gttgggatac gccgatcagc ttgttctcag ccacgatgtc ttccttaaac aaatgtaggc
      721 taaaaatggc ggtaatggct ggggatttgt tccagatgtt tttctggcct atctggcgga
      781 gcgcggcgtc gataaaacga tcctcaaaaa actctgtatc gataatcccg gacgattatt
      841 gaccgcataa aaccattatc ccccggcaca ctgcgccggg ggatgtcaat taataccccg
      901 ccgttaaatc atccaccaag cgcgagtcgg atgcgccgta caactcacca tccgacccaa
      961 ccataatgct ttgcgtgctg cccatcgcct cattcagcgc cactttctga ccttttgctt
     1021 ccagcagctt gagcgtatcc ggactaaacc cttttcaaca cgcagctcgt ccggcaacca
     1081 ctgatggtgg aaacgaggcg cattggtcgc ttcggcaaca ttcattccac aattgatgct
     1141 gttcaccacc atttgcagca cggtagtgat gattcagcta ccggtaacaa agtgagtaca
     1201 taaaggccat actgtcacga acagtgcaaa agtggctctt ccgcaaccta ggtgaagatt
     1261 ttcatgccag tggactcatt accctccgcc taagtagttc aaatccggct cgaccataca
     1321 tctggcgtat cagcaccttc aggcgattta catgaccttc gacaacacca ttactccagc
     1381 gactgctgat tgcctcacat atagccgcag catcagcttc catccccgca gctacgcacc
     1441 gaagttctgc tgagccgctt tcgtggactc gagtgaacca gctgcttagc tgtgatttat
     1501 tttaggtttt cagaatacgg tagaactcga gtaccagttg ctgtgctatt ttcaactccg
     1561 gttctttttc acacatcaga ctaataaatc gggatgcata attttcttcg cccctgatta
     1621 ttctccaggg catcaacccg cggcttaccc ggaacactga aggaagtctt gcggcagtag
     1681 taaccggtgg gttccagcct ttacgccatt tggcaacagt atccctgacg atggtttcac
     1741 tgcctgtccc cccccctggg ccaccatttc ccgccatatc cggctggcat tataattacc
     1801 actttctcgt tgctctttta accactccct ccatgggtcc agaagccctc gctttggagg
     1861 ccgggttgac atttcaggga atgttcctga acgcacccag cgactgactg tcactcgcga
     1921 caggcctgta atacgggata tttccctgaa actacatccc ttgttatgca gggccataac
     1981 ctctgtccaa tgctgatgcc gttttttgcg ggtttgctgt ttaaggcgtt ccggacgacg
     2041 gagcgatgct acaggcacag atatttctgg ctcaggtgat ttcttcagtg ataactctct
     2101 gacaacaaga cgtatcagag gcatatgtct gtacatcatt cgttcaggct catcaccaat
     2161 attttttagc aggtgccatc gatcggccac ctgtctggcc tgaggtgcac cttcacgtgc
     2221 tgctgtcgca tagactccac tgcgatcacg cgagacaacc tgtatttccg gatattttct
     2281 gaaccaggtc gccagcgtac gctgatcacg gccgggaagc aggacgaggg gacggtgagt
     2341 atcaagattg actattaaca taccgcagca gtggccccga tgccacgccc actcatcaat
     2401 accgacatga ggcgcgcctg actgtttcgt ctccggggta ttgataaccc tgcgaagaag
     2461 agtatcagca ctgcaacgga gacccgctgc cgttgcagcc cgttttccag cctcacctcc
     2521 agctattaat cccagttgac gttgtaaatt ttgtaacgcc tgtgaagact gttggtgtga
     2581 accggcgaag ggagcaagcg actcggcaaa aattttacgt gaacaagctg ggttacggca
     2641 gtaccagtgg cggacggaaa atacaagcca tagcgtctgt cctgatcagg gtagatgctg
     2701 tatccggcgc cgacaacaac tatgaacaga gtcgctacgc ttaaagcatt caggacattg
     2761 tgctgttttg cgttttccgt ggagatggag ggttataccg tcagagctaa taatttgtcg
     2821 actgcatcgc catccgcaag gtaactgtag aagtgtctta agagagtgca ttgggttgcc
     2881 cataacttaa aacagcttag atctttgttc gccagaaagt ttcatccatt ttcttccacc
     2941 caggttgcgg aagaaccact tttcatccgt cgttgacaga tgtgaagtat gcgggagaaa
     3001 aagtgaatat ctaactgtaa taactcagac cggtacgagt actcggggcg tctacaatca
     3061 ttcagggcaa agtgctaaaa tttctctatc ccgggatgcc cctaccaacg cagcactccc
     3121 ggagatatca ttacagctct gcaactgatt tccgcgactg aaccgctcag ttcctatcct
     3181 tccagttgc
//
LOCUS       DAZGJW010000301         3183 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZGJW010000301 DAZGJW010000000
VERSION     DAZGJW010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.304"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..143)
                     /locus_tag="ACHKIK_005134"
     CDS             complement(<1..143)
                     /locus_tag="ACHKIK_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFO9086093.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEA"
     gene            complement(873..1070)
                     /locus_tag="ACHKIK_005135"
     CDS             complement(873..1070)
                     /locus_tag="ACHKIK_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9086094.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(1136..1406)
                     /locus_tag="ACHKIK_005136"
                     /pseudo
     CDS             complement(1136..1406)
                     /locus_tag="ACHKIK_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1711..1899
                     /locus_tag="ACHKIK_005137"
     CDS             1711..1899
                     /locus_tag="ACHKIK_005137"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086095.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            2227..2421
                     /locus_tag="ACHKIK_005138"
     CDS             2227..2421
                     /locus_tag="ACHKIK_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086096.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            2764..2892
                     /locus_tag="ACHKIK_005139"
     CDS             2764..2892
                     /locus_tag="ACHKIK_005139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086097.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
BASE COUNT          969 a          721 c          699 g          794 t
ORIGIN      
        1 gcttctgctg ctttattcgc gaatttgcca gcgctactaa ttactacatc atgtccaatt
       61 acacgaaagg cctgagccag agaaatcaat gggtataaca aaccgtacag cggtgggcca
      121 ataaaaagaa tacgttttcg cattaaaaaa gtccgtcttc tgcttacctg tttatttttc
      181 tatctctaag aaaacataat taatattatt tttatgtgag cttatgcaca aaaataacca
      241 agtatattat cttctggtag agattcagga tacagtttgt aggagctttc ttttttgaac
      301 aatgatttgc aggacagtta tgctaaacta gcggaatcag tatcacaaca ataaatgact
      361 gatcatacct gaaatttgaa ttaacaggca tcatgaatgt caatagtcta agcggtgact
      421 acttatatcg cttgcataaa tactaaacac tgactagaaa agtattggat aaccccgtca
      481 ctaaaagtaa cgcttcggtt atttgctcgg aatgtattaa cgccaagatt gttagccgtt
      541 acgccttata tcactggcgc tgacaaatgt ttcttttcta atttactgaa ccggcgcaga
      601 cacgcaggaa agcacgccgg caccggtgta atgccagttc taatcaataa gaatggaatg
      661 acgaatcagt caggaaggga taacaacccg ctgctgaacg gaaggtatca gcaggaagat
      721 gaacgggaat atcgccagca ccaagggcag ggccgggttc cacaatacca gcgctggcat
      781 tttgcacatt gccagcgtac cggcattaca acacgtttca tgctgctgcc ccccaggatt
      841 cctcgattcg ttgctacatc cactgctcta cttcacttcc actgctccac ttcacttttg
      901 taccagcggc tgctgcgccc cagtttgatg ggtttaggga aatggccttc actgatcaac
      961 ttgtaaaacc atttgtcggt cataccggtg aaactggtga tatagttcat gtcgatcagg
     1021 gaattatcgg cacgcagaaa tacgctgtcg tggcttgttg tagtcaacat ggtcagaatg
     1081 tcctgtgaga tttatgaaga ttgtgcgaat gcgggaaatc tactgagctg tgctttcaga
     1141 actggcctgt tacgggatac cagggattac cggcggggta acgggcttcc ggatcataca
     1201 caccacgatt atcgcggaca aaatcactga acgcccatat cacctcttta agtatgtctt
     1261 cgcagtccgg taaatgacga tccagcgcca catcccgggt ggtacagctt tgatgcgccc
     1321 ggtgacacaa agcccggatt gttccagaca tcctgaatca aacgccccag attaggggcg
     1381 tcgaaatatg cctctctgac cattatattc cggcgtatag gtagcaggtc tgaagtgaca
     1441 atgcgtcacc tgacgttaaa agtcacccaa gatgacccca gtgggggcaa caagtataaa
     1501 accgtttttt gattaagtgg tcggatatga aaaggggaag gtttatgtga aaaatacctg
     1561 tgttttcttt gaaaacacac aacagtatca ggttatttat gtttatacag tgcgttccaa
     1621 actgacctga ataattacgc aagttgcaac aataaaacat ttttgccgta gtgtgaggta
     1681 ctccggtaaa ttgcttccca ggtgcagaca atgactaacg aagaactaca ggcaatcgca
     1741 ttatccatgc ttgagcgcca gcgcgaaaaa gtacaccagc aacgagagtc tttgcttaac
     1801 ccatctccat tcaccaggcc aaaacgattg gagcttgaag cattcttgga caaataccca
     1861 cggcggctta aatcaggcaa aaccagacca ccaggctgat agctcacagt ttgttctccg
     1921 attcaataaa acggataaga accatcagcg cggtagcgta aggcactaca gcgcattgct
     1981 ttacgagtaa taacaatgga tttatcacca gaagacgcaa agtttttacg agaactgcaa
     2041 caccgtatgc tcagagaagc cattatcgaa cagcaaacgg gcatgagcgc catagaccca
     2101 ttgcgataca aacgggcctc atctccttta gttgacgaca attccaatgc ggataaaaca
     2161 cgtccaccac actgattaca tttttcatta gcacagtggt gtgatgcagc atacgaggaa
     2221 aaccggatga atgagaacaa aataaaaagg ctggagcaac tgttacaggc acgccaacgg
     2281 gagtttgcca ctaaatgggg acgtgccgcg ctgccacatg aacgcctaac ctattttcca
     2341 cttgaagacc tcattaaaaa taatggcaaa aaaatcaggt ctgataagaa acacttctta
     2401 gaatgcaatg aagacaaata acaacaatct gcatattgcc accaaccaca gatgtggcat
     2461 cgttatcagc gcacttgatt ttctttatat ccactgacga gtttgccggt cattctggta
     2521 tcatgcagat atttacggcg tacaagcaca ctagcatgac attgtaataa ggcctgagcc
     2581 taacgcttta atgtgtgtca tacactcagt ggcgtaatcg ttgacaatat ggtgcatgta
     2641 atcgtaagcg tccagcgaaa tccatctggt cataatctga agcatccgac aaagtggtgt
     2701 ccaccaaata agtgatctgc gcccaatatc ctgagccgga actttgacaa aggccaacga
     2761 aaaatgggag cggatgttac agagttctcg gtacagggga aaagtggtac ctgtcgccag
     2821 ttctccgatc tgtttaaccg ggaaattatc tcctacagct tgtcagaaag gtcggtgatg
     2881 gagcatcgtt aatacgatgc tatacgatgc attcacagtg cccggccaga ggatgccccg
     2941 tcgctgcata tggatcaggg ttggcaatat cgaattgcag gctataggca aagttaaagc
     3001 cccatggagt agcacaaaat atgccgcaca aaggaaacgg tctgaataac gcagtgatga
     3061 agaacttctt cagcacactg ctaaaacgca tagtgatcga gcgctgattc tggcgaacaa
     3121 ctgaactaat acatcagaat ctgcattatg ttaaataaat ataaaaagat ggtttaaata
     3181 ccc
//
LOCUS       DAZGJW010000302         3178 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZGJW010000302 DAZGJW010000000
VERSION     DAZGJW010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.305"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            82..197
                     /gene="rrf"
                     /locus_tag="ACHKIK_005140"
     rRNA            82..197
                     /gene="rrf"
                     /locus_tag="ACHKIK_005140"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            212..287
                     /locus_tag="ACHKIK_005141"
     tRNA            212..287
                     /locus_tag="ACHKIK_005141"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:245..247,aa:Thr,seq:ggt)
     gene            327..442
                     /gene="rrf"
                     /locus_tag="ACHKIK_005142"
     rRNA            327..442
                     /gene="rrf"
                     /locus_tag="ACHKIK_005142"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(506..1264)
                     /gene="yhdZ"
                     /locus_tag="ACHKIK_005143"
     CDS             complement(506..1264)
                     /gene="yhdZ"
                     /locus_tag="ACHKIK_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO9086098.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(1272..2375)
                     /gene="yhdY"
                     /locus_tag="ACHKIK_005144"
     CDS             complement(1272..2375)
                     /gene="yhdY"
                     /locus_tag="ACHKIK_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO9086099.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2385..>3178)
                     /locus_tag="ACHKIK_005145"
     CDS             complement(2385..>3178)
                     /locus_tag="ACHKIK_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO9086100.1"
                     /translation="FRNIPPLLQIFFWYFAVLRNLPGPRQAVSAFDLAFLSNRGLYIP
                     SPQLGDGFIAFILAVVMAIILSVGLFRFNKTYQIKTGQLRRTWPIAAVLIIGLPLLAQ
                     WLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQAVPYG
                     QHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAAIGYPDMVSLF
                     AGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          862 a          837 c          783 g          696 t
ORIGIN      
        1 tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc
       61 ggtctgataa aacagaattt gcctggcggc cttagcgcgg tggtcccacc tgaccccatg
      121 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga
      181 gtagggaact gccaggcatc aaatttagcg tgctgatatg gctcagttgg tagagcgcac
      241 ccttggtaag ggtgaggtcc ccagttcgac tctgggtatc agcaccactt tttaggttaa
      301 agttcggcag attagaaaag aatttgtctg gcggcagtag cgcggtggtc ccacctgacc
      361 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg
      421 cgagagtagg gaactgccag acatcaaatc aagcaaaaag gccatccgaa aggatggcct
      481 ttttgctttt cgaactaaca ttcaattaat ggattacctg cgataaaaat gccctcgtac
      541 gctctgattt aggatgcgta aaaaattcat caggtgcagc ttgctccact atttccccac
      601 gatccataaa aatcacccgg tcagcgacgg ttcgcgcaaa ccccatctca tgcgttacac
      661 acaacattgt catacccgat tgcgccagcc caatcatcgt atccagcacc tctttcacca
      721 tctccggatc gagcgccgac gttggctcat caaacaacat aattttcggc ttcatacaca
      781 gcgaacgcgc aatggcaacg cgttgctgct gaccaccaga aatttgtccg ggaaacttat
      841 gcgcatgttc ggcaattctc acccgctcaa ggtaatgcat cgccagagct tcagcctctt
      901 tctttggcat cttgcgtacc caaatcggtg ccagggtaca gttctgtaga acggtcagat
      961 gagggaagag attgaaatgc tgaaagacca ttcccacttc ctgccggacg cgctcaatat
     1021 tgcggatatc ttcattaagt tcgatgccat ctaccacgat ccgtccctgt tgatgttctt
     1081 ccagatgatt aatacaacga atggttgtcg atttaccgga acctgaaggg ccacacagaa
     1141 cgatccgttc tcccggttgc acggttaaat ttatattttt caaaacatgg aattgtccat
     1201 accatttatt gacgttttcc agcgtaatca tcgcgttagc aggttgcagt aaaatttggc
     1261 tcatagtgtc ctcaatgcgg tgtacgcccg gtgttaaaac gtttttccag atgctggcta
     1321 tagcgcgaca tgctgaaaca gaagatccag tagatcagcg cggcgaaaac atacccttcc
     1381 gtcgacatac ccaaccaggt gggatcaacg gttgcctgct gaacgctact gaaaagatcg
     1441 aacaacccga taatgatcac caggctggta tctttgaaga gtgcgatgat ggtatttacc
     1501 agcccaggaa ttaccagctt caacgcctgt ggcagaataa ccagcccctg agttttccag
     1561 taacccaacg ccagcgactc tgccgcttca tactgccctt taggcagtgc ctgtaatccg
     1621 cctcgcacga cttccgcaac atatgctgac tgaaacagga tcacgccaac cagcgcgcgg
     1681 atcagtttgt cgatactggt gccttctgcc ataaacaacg gcagcatgac cgaagacata
     1741 aacaatacgg taatcagcgg tacgccgcgc cagaactcga taaaaatgac cgataagata
     1801 cgcacaatcg gcatatggga gcggcgacct aacgccagta agatccccca cggtagcgcc
     1861 cccgcaatcc caacggatgc aataattaaa gttagcgtca gcccgcccca ttgccgggtt
     1921 tcaacccgct ccagaccaaa aaaaccgcca tacatcagcc accagacaat cagtgggtaa
     1981 atcaccgccc agaccgcaat atagcgaccg cgatgcggga gtattttcca gaacattggt
     2041 gcgattgaca caaggccaat cagtaatgcc aggttaattc gccagcgttg gtcgtgtggg
     2101 taaagtccat acataaactg accaaatcgt tcgtggatga agacccaaca ggcaccggct
     2161 tttgtgcagt cggcacgcgt tgagccaacc caattagcct gcaaaaatgc ccagttcagc
     2221 aacggtggga tcagttccca catcaaccat atgcagccaa tagtcagcag gctattgctc
     2281 cagctggaga acagattttt tcgcacccac accatcgcgc ggcttgagtt atggctcgct
     2341 gggcgcgagg gctgagacag caatactttt gtcatcatga ctccttagcg ttcaacgatc
     2401 gcgatgcggc ggttatagat attcatcagc agcgagatag tcaggctgat aatcagatag
     2461 accgacatgg tcatggctat cgtctcgatg gcttgccccg tctggttcag cacggtgcca
     2521 gcaaacagcg aaaccatatc gggatagcca atagcggcgg caagagagga gtttttgacg
     2581 atgttgagat actggctggt taacggcgga ataatcactc gcagtgcctg gggaataatg
     2641 acctggcgta gcgtaaccgg gttgggtaat cccagtgacc gagccgcttc atgttgacca
     2701 taaggcactg cctggatccc agcgcggata atctcggcga taaatgcaga tgtataaacc
     2761 gaaagtgcca gcgttaaggc tgccagttca ggaattaaaa ccatcccgcc gcggaagtta
     2821 aagcctcgta gggctgggac atcccagtgt agtgctgcgc caaaaagcca ttgcgccagt
     2881 aaaggcaaac caatgatcaa cactgcggcg atcggccagg tgcggcgcag ttgtccggtc
     2941 tttatctggt atgttttatt aaagcggaat agcccaacag aaaggattat agccataaca
     3001 acagccagga taaacgcaat aaatccgtct cccagctgcg gtgacggaat ataaagccca
     3061 cgattgctca aaaaggccag atcaaacgcg ctcacagcct ggcgtggtcc gggcaaattg
     3121 cgcaacacgg caaagtacca gaagaagatt tgcagcagcg ggggaatatt acggaaga
//
LOCUS       DAZGJW010000303         3172 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZGJW010000303 DAZGJW010000000
VERSION     DAZGJW010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.306"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..2474
                     /locus_tag="ACHKIK_005146"
     CDS             <1..2474
                     /locus_tag="ACHKIK_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO9086101.1"
                     /translation="ELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAEDRENVNLSQFE
                     TDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTLLGFTDEIIEEA
                     QQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPPELWDHGIAGAF
                     LDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFNNGKSQANNLDF
                     PRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPPDLRGYAPQITG
                     VAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEEDGQVTQWQVAS
                     NSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNVSLYGGSVLTNR
                     DYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSKRFESTGSQLTF
                     AGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLNTYVSLARNTYW
                     DASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNISIPWGTSRTLS
                     YGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRASYQHNTENGRL
                     YLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDADGAEDIPLNNKR
                     AVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEGAIGYAKLDTRK
                     GYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQDDSKLTLRWGDK
                     SCFIQPPNSSNLTTGTVILPCISQN"
     gene            2490..>3172
                     /locus_tag="ACHKIK_005147"
     CDS             2490..>3172
                     /locus_tag="ACHKIK_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9086102.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLS"
BASE COUNT          983 a          668 c          644 g          877 t
ORIGIN      
        1 gcgaattgat taaaaatatt tattgttcat tatccgttct catcattggt tgtgcgtcag
       61 cttatgccgt tgaattcaac aaagatttaa tcgaagccga agatcgtgaa aacgttaacc
      121 tttcccaatt tgaaactgat ggccaattac ccgtaggtaa atactcatta aacgctctta
      181 ttaataataa aaggacgcca atccatctgg acctgcaatg ggtattaatt gataaccaaa
      241 ctgcagtttg tctgactcca gagcaattaa cattattagg ctttactgat gagattattg
      301 aagaggctca gcaaaacctg atcgatggtt gttaccctat cgaaaaagaa aaacaaatta
      361 caacttatct cgataaaggg aaaatgcaat tatccatatc tgcacctcag gcatggttaa
      421 aatacaaaga tgcaaactgg acgcctcctg aactttggga tcatggtatt gctggggcat
      481 ttcttgacta caatttatat gcctctcatt atgcaccaca tcagggcgat aattcacaaa
      541 atataagttc ctatgggcag gctggggtta atcttggggc ctggcgcctg cgtactgatt
      601 accagtacga tcagtcattt aacaatggca aaagccaggc gaacaacctg gattttccgc
      661 gtatttattt gtttcgccca atcccagcaa ttaatgcaaa actaactata ggtcaatacg
      721 atactgaatc ctctattttc gactctttcc atttttctgg cgtttcgttg aaaagcgatg
      781 agaatatgtt gccgcctgat ctacgtggtt acgcaccgca aatcacgggt gttgcacaaa
      841 cgaatgcaaa ggtcactgtc tcacagaaca accgtattat ttatcaagaa aatgttcctc
      901 ctggcccatt tgctattact aatttattca atacattaca ggggcaactg gacgtcaagg
      961 ttgaagaaga ggacgggcaa gtcacgcaat ggcaagttgc atctaatagt attccttatc
     1021 tgacgcgtaa agggcagatt cgctacacca ctgctatggg taaaccgacc agcgttggtg
     1081 gtgattcctt acaacaaccc ttcttctgga ctggtgaatt ctcatggggt tggctgaaca
     1141 atgtatccct gtatggtggt tcagttttaa caaaccgtga ttatcaatct ctggctgccg
     1201 gcgttggctt taaccttaac tcattgggtt cattatcttt tgatgtcaca cgatctgatg
     1261 ctcagttgca taatcaggat aaagaaacgg gttatagcta ccgcgctaac tattcaaaac
     1321 gttttgaatc taccggtagc cagctcactt tcgctggtta ccgtttctct gataaaaact
     1381 ttgtgacaat gaatgaatat atcaatgaca ctaaccatta cacgaattat cagaatgaaa
     1441 aagagagtta tattgtcacg tttaaccagt atcttgaatc attaaggtta aatacatacg
     1501 taagtttggc tcgtaatact tactgggacg ccagcagtaa tgtgaattat tcattatcac
     1561 ttagccgcga ttttgatatc gggccattaa aaaacgtctc cacttctcta acatttagcc
     1621 gaataaactg ggaagaagac aaccaggatc aactgtacct aaatatttcg attccctggg
     1681 gaaccagtag aacattgagc tatggtatgc aacgaaatca ggataacaag atttcgcata
     1741 ctgcttcgtg gtatgactct tccgatcgaa ataattcctg gagcgtttct gcttcaggcg
     1801 acaatgatga attcaaagat atgaaagcgt cactacgcgc cagttatcag cataataccg
     1861 agaacggtcg tctctatctc tccgggacat cacagcgaga cagttattat tctctgaatg
     1921 ccagttggaa tggttcattc acggcgactc gtcacggagc cgctttccat gactatagtg
     1981 gtagtgctga ctcgcgtttt atgatcgacg cagatggtgc agaagatatt ccgttgaaca
     2041 ataaacgcgc ggtaactaat cggtatggca tcggagttat tccatcagtc agcagttaca
     2101 taacaacatc attaagcgtt gatacccgaa atcttccaga aaatgtggat atcgaaaact
     2161 cggttatcac caccacctta accgaaggtg ctattggcta cgccaaactt gatacccgca
     2221 agggctatca aatcatgggg gttattcgcc tggcagatgg tagtcaccca ccactgggga
     2281 ttagcgtaaa agataaaacc agccataaag aattaggact ggttgctgat ggcggctttg
     2341 tatacctcaa cggcattcag gatgacagca aacttacttt acgctggggt gataaatcct
     2401 gttttattca accacccaat agcagcaact taaccaccgg aacggttatt ttaccgtgta
     2461 ttagccaaaa ttaattcagg atgaaactaa tgcgctactt aaacacaaag aatctcattg
     2521 cagcaggagt attactcgcc tgcatgagca gcattgcctg gggtgccatc atcccggacc
     2581 gtacccgtat cataatgaat gagtcagata aaggggaagc attaaaactt actaatcaaa
     2641 gcaaaaatct accctatctg gcgcaaacat ggatagaaga tacgaaaggc aataaatcac
     2701 gtgactttat tgtcacggtt ccccctatgg tacgtttaaa tcccagtgag caaattcaaa
     2761 taagaatgat aagtcaggaa aaaattgctc aactgcctaa tgacagagaa acgctcttct
     2821 attttaacgt gcgagaaata ccgccaaaaa cagataaaaa aaatgtgatg caagtcacta
     2881 tgcagcacgc attgaaacta ttctggcgac caaaagctat tgaattagaa gatgatgggg
     2941 taatgtctta cgaaaaagtt gaaatcatcc gtagaaatga tggttcgatc cgctttaaca
     3001 ataagatgcc ttatcacgtt acgctgggtt atatcggaac aaacggaatc acaatgttgc
     3061 cacaaacgca aagtttaatg gtaacccctt atagccatgc agatacgcag tttaaaaatg
     3121 ttccgtcagc ttttcaggtt ggttatatca atgattttgg tggattaagt tt
//
LOCUS       DAZGJW010000304         3167 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZGJW010000304 DAZGJW010000000
VERSION     DAZGJW010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.307"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            233..565
                     /gene="hdeA"
                     /locus_tag="ACHKIK_005148"
     CDS             233..565
                     /gene="hdeA"
                     /locus_tag="ACHKIK_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO9086103.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VEESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            681..1007
                     /gene="hdeB"
                     /locus_tag="ACHKIK_005149"
     CDS             681..1007
                     /gene="hdeB"
                     /locus_tag="ACHKIK_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO9086104.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            1071..1718
                     /locus_tag="ACHKIK_005150"
     CDS             1071..1718
                     /locus_tag="ACHKIK_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO9086105.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGIAGVKGVSIS"
     gene            complement(1760..2290)
                     /locus_tag="ACHKIK_005151"
     CDS             complement(1760..2290)
                     /locus_tag="ACHKIK_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO9086106.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(2446..3012)
                     /gene="slp"
                     /locus_tag="ACHKIK_005152"
     CDS             complement(2446..3012)
                     /gene="slp"
                     /locus_tag="ACHKIK_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO9086107.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          932 a          623 c          686 g          926 t
ORIGIN      
        1 aagaaaatcc cctgctatca atctatgcca aaaacgcgtc taagaatgca gtcgatttaa
       61 taaaaatttc ctaattgcag tatctgatgc atctgtaact cattgtattg aaataaaaat
      121 atctgatttt gatattttcc atcaacatga catatacaga aaaccaggtt ataacctcag
      181 tgtcaaaatt gattcgtgac ggctctttca ctttatagtt gaggatatta cgatgaaaaa
      241 agtattaggc gttattcttg gtggtctgct tcttctgcca gttgtgagca atgcagcgga
      301 tgcgcaaaaa gcagctgata acaaaaaacc ggtcaactcc tggacctgtg aagatttcct
      361 ggctgtggag gaatccttcc agccaactgc agttggtttt gctgaagcgc tgaacaacaa
      421 agataaacca gaagatgcgg ttttagatgt tcagggtatt gcaaccgtaa ccccagctat
      481 cgttcaggct tgtactcagg ataaacaagc caactttaaa gataaagtta aaggcgaatg
      541 ggacaaaatt aagaaagata tgtaattccg ggaatgcgtt acatcgtact tccttgcata
      601 ttgaacaggc cggaatatct tctttaaaag cagctattcc tcctgttcat atataatctc
      661 tatattgaat gggttacaaa atgaatattt catctctccg taaagcgttt atttttatgg
      721 gcgctgtagc ggctttgtca ctggtgaacg cacaatctgc gctggcagcc aatgaatccg
      781 ctaaagatat gacctgccag gaatttattg atctgaatcc aaaagcaatg accccggttg
      841 catggtggat gctgcatgaa gaaacagtat ataaaggtgg cgataccgtt actttaaatg
      901 aaaccgatct cactcaaatt cctaaagtga tcgaatactg taagaaaaac ccgcagaaaa
      961 atttgtatac cttcaaaaat caagcatcta atgacttgcc gaattaatga ggtacaagta
     1021 aaaaggagta gcaagttgag ccatcttgct gctccttttt gcatttttat atgacagcag
     1081 aatttattat acgtcttata ctcgcggcaa ttgcctgtgg cgctattggc atggaaaggc
     1141 aaatgcgcgg caaaggagca gggttacgca cacatgtatt aattggcatg ggaagcgccc
     1201 tgtttatgat tgtttcgaaa tatggttttg ctgacgtgct gtctttagat cacgtcggac
     1261 tcgaccccag ccgtattgct gctcaggtgg tgacgggcgt cgggtttatc ggtgcaggta
     1321 acattctcgt tcgtaaccaa aatattgtag gtctgacgac ggcagcggat atctgggtga
     1381 ccgccgccat aggtatggtt attggcagcg gtatgtacga actgggtatt tatggttcag
     1441 tgatgacctt gttggtgctg gaagtcttcc atcaattaac cttccgcctg atgaacaaaa
     1501 attaccattt acagctgaca ttagtgaacg gtaatacggt atcaatgctc gactggttta
     1561 agcagcaaaa aataaaaacg gatttagttt cgttgcagga aaatgaagat catgaagtgg
     1621 tcgccattga tatccagctt catgccacaa cgtcgataga agacctgttg cgattattaa
     1681 aaggtatagc aggggtgaaa ggcgtttcaa ttagctaatc tgtgtggcag cgtagccaga
     1741 ctcaccgtaa gcctgaaatt cacaccagat aatcaatatg ctgatggcgt accagctcat
     1801 tgatccgctt aagattgagg atcatcatga tattgtgttt atggcagtag aacgttttta
     1861 gcgatatttt cagtttctcc agaatatctt cagttgtcat ttgattcgca atgcagctca
     1921 gaacctgatg ttgcttgcgc gaaagtgaca cattcgcttc aggcacagag cagtaactgc
     1981 cattgatgag tgcgacaaaa tcaatgatag tgatagtccg cggaacaaag gtcaccggta
     2041 ctttccccag acagcgttta atattaaagg gggagaaaac aatgacatgg acaggtttta
     2101 atcgttcaat ttgagtgaga aaattagaat gaaatacatt attcattaat gtatcaataa
     2161 tgacgaaggc attctgatgc aacattacgt cgatctcttc ttcgttctgt atatgaacga
     2221 cattaccttt actcagaatg tttttcatcg cggtgaagaa catcgtatcc ctggtaatta
     2281 taagaaacat aaccgactcc tggtgcggac attaacttta taatcagttt gccaaaggta
     2341 attaacagga cagggcttat aataataata gttagcttac tgtatattaa ttttatgtaa
     2401 tacatgatgc agatgctgag aaagcatctg catctttcgg tggtgttatt tgaccagctc
     2461 aggtgttacc tgactcaccg cattggtgta gtaaggcgca ccccagcccg gttccggcca
     2521 gaatgcacca tagccgtaat cccacaggtt ataggtggta ttaaccactt ctctcaaatg
     2581 ccacacctgg atgccctgca tattcacttc caggaagtta tacgggactt tattgataaa
     2641 gcctggttgt tcaccctgaa tggtaccgag gatggtaaca aagtgattac gatagttcac
     2701 tggatcaagg aagccgcttt gtctggcgag cagtcggccc tgatagttgg cttcaatatc
     2761 aggcttcgca tagctatcca acggtaatac agcgatttct aacaacgtat ccgttttgcc
     2821 attgataacg ttgataacct tcccaccaaa gcgcgcttgt tgaccaacat ataaccccgg
     2881 ctggttatga acagcaacaa aacttttttg aatatcaggt tggttattgc ctttgatatt
     2941 ttgcggaatt gaactacatg cggcaagcaa aaatgaaagg ctgaggatga gtgcaccttt
     3001 tgtcatgttc atgttactat ccttatcaac aaatattaaa gccataatag atatttcttt
     3061 atagttttca tctgattctg aggtgatatg cctgatagta acgtaaacct gtctgatgtt
     3121 tatatccctt ttgtttagtt caattccatt gattttgggg cgtggca
//
LOCUS       DAZGJW010000305         3113 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZGJW010000305 DAZGJW010000000
VERSION     DAZGJW010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.308"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1820
                     /locus_tag="ACHKIK_005153"
     CDS             <1..1820
                     /locus_tag="ACHKIK_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO9086108.1"
                     /translation="NYYLPKESAYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARV
                     GYSKMLLGVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGK
                     KHRDNTLTMKRFSNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFL
                     GDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDK
                     ETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSS
                     GEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIG
                     ERPDAEVMAERKEYYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQII
                     MGRHDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRV
                     IPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVH
                     FPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISI
                     SRWQLDLSALLASLQEEDGAATNKKTLAEYARALSGEDE"
     gene            1817..2023
                     /locus_tag="ACHKIK_005154"
     CDS             1817..2023
                     /locus_tag="ACHKIK_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO9086109.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2020..>3113
                     /locus_tag="ACHKIK_005155"
     CDS             2020..>3113
                     /locus_tag="ACHKIK_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9086110.1"
                     /translation="MKMSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTR
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYSAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAG"
BASE COUNT          737 a          794 c          940 g          642 t
ORIGIN      
        1 ccaattacta tctcccgaaa gaatccgcat accaggaagg gcgctgggaa acactgccct
       61 ttcagcgggc catcatgaat gcgatgggca gcgactacat ccgcgaggtg aatgtggtga
      121 agtctgcccg tgttggttat tccaaaatgc tgctgggtgt ttatgcctac ttcatagagc
      181 ataagcagcg caacacactt atctggttgc cgacggatgg tgatgccgag aactttatga
      241 aaacccacgt tgagccgacc atccgcgata ttccgtcgct gctggcgctg gctccgtggt
      301 atggcaaaaa gcaccgggat aacacgctca ctatgaagcg tttttccaat ggtcgtggct
      361 tctggtgcct gggcggtaaa gcggcaaaaa actaccgtga aaagtcggtg gatgtggcgg
      421 gttatgatga actggctgcc tttgatgagg atattgaaca ggaaggctct ccgacgttcc
      481 tgggcgataa gcgtattgaa ggctcggtct ggccaaagtc catccgtggc tccacgccca
      541 aagtgagagg cacctgccag attgagcgtg cagccagtga atccccgcat tttatgcgtt
      601 ttcatgttgc ctgcccgcac tgcggggagg agcagtatct taaatttggc gacaaagaga
      661 cgccgtttgg cctcaaatgg acgccggatg acccctccag cgtgttttat ctctgcgagc
      721 ataatgcctg cgtcatccgc cagcaggagc tggactttac tgatgcccgt tatatctgcg
      781 aaaagaccgg gatctggacc cgtgatggca ttctctggtt ttcgtcatcc ggtgaagaga
      841 ttgaaccgcc tgacagtgtg acctttcaca tctggacggc gtacagcccg ttcaccacct
      901 gggtgcagat tgtcaaagac tggatgaaga cgaaagggga tacgggaaaa cgtaaaacct
      961 tcgtgaacac cacgctcggt gagacgtggg aagcgaaaat cggcgaacgt ccggatgctg
     1021 aagtgatggc agagcggaaa gagtattatt cagcgcccgt tcctgatcgt gtggcttacc
     1081 tgaccgccgg tatcgactcc cagctggacc gctacgaaat gcgcgtatgg ggatgggggc
     1141 cgggtgagga aagctggctg attgaccggc agattattat gggccgccac gacgatgaac
     1201 agacgctgct gcgtgtggat gaggccatca ataaaaccta tacccgccgg aatggtgcag
     1261 aaatgtcggt atcccgtatc tgctgggata ctggcgggat tgacccgacc attgtgtatg
     1321 aacgctcgaa aaaacatggg ctgttccggg tgatccccat taaaggggca tccgtctacg
     1381 gaaagccggt ggccagcatg ccacgtaagc gaaacaaaaa cggggtttac cttaccgaaa
     1441 tcggtacgga taccgcgaaa gagcagattt ataaccgctt cacactgacg ccggaagggg
     1501 atgaaccgct tcccggtgcc gttcacttcc cgaataaccc ggatattttt gatcttaccg
     1561 aagcgcagca actgactgct gaagagcagg tcgaaaaatg ggtggatggc aggaaaaaaa
     1621 tactgtggga cagcaaaaag cgacgcaatg aggcgctcga ctgcttcgtt tatgcgctgg
     1681 cggcgctgcg catcagtatt tcccgctggc agctggatct cagtgcactg ctggcgagcc
     1741 tgcaggaaga ggatggtgca gcaaccaaca agaaaacact ggcagaatac gcccgtgcct
     1801 tatccggaga ggatgaatga cgcgacagga agaacttgcc gctgcccgtg cggcactgca
     1861 tgacctgatg acaggaaaac gggtggcaac ggtacagaaa gacggacggc gagtggagtt
     1921 tacgaccact tccgtgtctg acctgaaaaa atacattgct gagctggaag tgcagaccgg
     1981 catgacacag cgacgcaggg gaccagcagg attttatgta tgaaaatgtc caccattccc
     2041 acccttctgg ggccggacgg catgacatcg ctgcgtgaat atgccggtta tcacggcggt
     2101 ggcagcggat ttggtgggca gttgcgggcg tggaacccac cgggtgaaag tgtggatgca
     2161 gccctgctgc ccaactttac ccgtggcaat gcccgcgcag acgatctggt acgcaataac
     2221 ggctatgccg ccaacgccat ccagttgcat caggatcata tcgtcgggtc ttttttccga
     2281 ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt
     2341 tcccgcgagg ttgaagcggc atggaaagag tttgccgaag atgactgttg ctgcattgac
     2401 gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt
     2461 aacggtgaac tgttcgttca ggccacctgg gatacccgtc cctcgcgact gttccggaca
     2521 cagttccgga tggtcagccc gaagcgcatc agcaacccga acaataccgg cgacagccgg
     2581 aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc
     2641 gaggacgggt atcctggctg gatgccgcag aaatggacat ggataccccg cgagttaccc
     2701 ggcggtcgtg cttcgttcat tcacgtcttt gaacccgtgg aggacgggca gacccgcggt
     2761 gcaaatgtgt tttacagcgt gatggagcag atgaagatgc tcgacacgct gcagaacacg
     2821 cagctgcaga gcgccattgt gaaggcgatg tatgccgcca ccattgagag tgagctggat
     2881 acgcagtcag cgatggattt tattctgggc gcgaacagtc aggagcagcg ggaaaggctg
     2941 accggctgga ttggtgaaat tgccgcgtat tactccgcag caccggtccg tctgggaggc
     3001 gcaaaagtgc cgcacctgat gccgggggac tcactgaacc tgcagacggc tcaggacacg
     3061 gataacggct actccgtgtt tgagcagtca ctgctgcggt atatcgctgc cgg
//
LOCUS       DAZGJW010000306         3079 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZGJW010000306 DAZGJW010000000
VERSION     DAZGJW010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.309"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1888
                     /gene="espL1"
                     /locus_tag="ACHKIK_005156"
                     /pseudo
     CDS             1..1888
                     /gene="espL1"
                     /locus_tag="ACHKIK_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            2060..2167
                     /gene="yniD"
                     /locus_tag="ACHKIK_005157"
     CDS             2060..2167
                     /gene="yniD"
                     /locus_tag="ACHKIK_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO9086111.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(2220..2978)
                     /locus_tag="ACHKIK_005158"
     CDS             complement(2220..2978)
                     /locus_tag="ACHKIK_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO9086112.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGHQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          929 a          699 c          606 g          845 t
ORIGIN      
        1 gatatcatta tcagaactca ttataagtct cctcatagat tgcacatcga tagcgacata
       61 ccaacacctt catcagagcc tattaataaa tttgcgcgcc agctcatcac cctacttgat
      121 acctctgact taagttcgat gctgtcatac tgtgttactc aggaatttac cgcaaactgt
      181 cgaaaaatat cacaaaattg ttattccact gcccttttta ccattaacct tgccacttca
      241 cccatccatg cagaaaatat actcattaca ttacactata aaaaagatat catttcctta
      301 ttactggaaa ccacgcctat taaagctaac catttgcgaa gcatactgga ttatattgaa
      361 caggaacagt taactgctga agatcgtaac cattgtatga aactgtctaa aaaaatccat
      421 agagaaaaaa ctatacaccc aacagtaaat ctcaatggta gtgcatgttt tttgcaatct
      481 ccttctgacg ctattttttg tcgccatctg tcattgcaat acgcccttga ttcattgaga
      541 aatggaaaag gcaaagtcaa cctgattaaa cattactcct ccgttgaatc catacagcag
      601 catgtcccct tagtccggga cgcggagttc agagcattac ttcgccatcc tcctgcaggg
      661 agtcgcgtta tcgcgagtaa ggattttggc ttcgctttag attttttctt ttgtcgaatg
      721 atggcaaaca atatcagtca tatgtccgcg attttatata tagacaatca tactttgtca
      781 gtaaggctac gaataaagca gtcagtgtat ggacaattaa actatgttgt gtccgtttac
      841 gacccgaacg ataccaacgt tgccgtcaga gacacccaca ggacagcacg gggctttctt
      901 tcgcttgata agttcatcag ttcaggtccc gatgctcaga cctgggctga taggtatgtt
      961 cgcaactgtg caattgctat tctgccccta ttacctgtgg gagttccagg ggctattttc
     1021 gcgggtattg catcacgaat gccatttgcc cctatacatc catcggcaat gttgttaata
     1081 atggctacag gccagactca acagcttatt acattattca aacagttgcc catactccct
     1141 gaaaaagaaa tcattgaaat aataactgcg cagaatagcg ttggtacacc tgctttattt
     1201 ttggctatga tgaacggaca tactgacaac gtgaaaatat ttatgcaaga aattcagtca
     1261 ctggtagata atcacatcat tcatgaagat aacctggtta aattactgca aactaaaagt
     1321 gctaacgaaa cacctggact ctatatctcc atgttgtatg gattcgatga aataatcgat
     1381 atctttctga atgcattaac cactccaata gcacaagaac ttttaaacaa aaaaatggtg
     1441 atggatattt tagccatgaa aacacgtgat ggtgaaccag ggttatttgc tgcaatggaa
     1501 aataatcacc ctttgtgtgt cacacggttc ctctctaaag tttatgggat cgccgttaaa
     1561 tataaactca gcaaaatcaa catcatggac ttattaaaag gcgcgacagc acagggaacc
     1621 cctgcattat acatcgccat gagcaagggt aatgaagacg tcgtgttatc ttatatatcg
     1681 acgctgggtg cttttgcaaa aaaaacattc ttttagtcaa catcagttat ttacactatt
     1741 ggccgctaaa aatcatgaca acatgtcagc tgttcatata gccattcatc ataagcatta
     1801 taaaactgta gaaacatatt atgctgctat aaatgtcatc agccaaagcc tgagttttag
     1861 tgctgatgaa ataaagacgt atttataacc agctatttac gatatatatc gatacataat
     1921 tttatttttt tcataaaacg cagaaacagt gaccaggtgt acaacgccac ccaacggctg
     1981 taataacaaa aaatcccttg atgcccgtcc cttttgttac actccgttat cacgcacaag
     2041 agatatgcag gacactggta tgccgactaa acgctttgat aaaaaacact ggaagatggt
     2101 ggtggtgcta ctggcaatct gtggcgctat gttgttgcta cgttgggcag caatgatttg
     2161 gggctgagca gtgacaaacg aagacagcca gaccgcataa cacggtccgg cagataattt
     2221 tacatgctgt agcccagcga taaggttgtc cgacggtcgg tatgttccgg tgcagactct
     2281 ggaggttcag agttccatgt gacgttgtaa gccaccttca gtccaaaatg ttcattaata
     2341 gcaacattta atgcgctttc ggagttcagc gttgtgtctt ccgcgccaaa gaccgaaaca
     2401 ccctgcgtaa atttagcgtt gtcagtcaac tgccatgcat aagccccgga ggcatagccc
     2461 agcggctggg tttcactggc attgtcggta tatttgtcgt aacgcacacc tggaccgaat
     2521 tcaaagcgga aactgtgtac cgggccattc aaaaactgat gaccataacc cgcggtcaac
     2581 acatcgcgct ctcgatagcc gttataacgg tctgtcagcc agcttgcctg accaaataaa
     2641 tagtcataat cagttaaatt aaaacggcta cgcccgcccg ccgcatattt ttctgaagaa
     2701 cgctcatcat tagaagaagt attactggcg ttcccccaca gcgaccaggc agtggtttgt
     2761 ccataccagg tcatggtggt gtcagccgta agtgaggagc ttttcgtatt gcctgattgt
     2821 gcaagatatc ctgcgttcag attaccttcg aaaggttttt tagcgctggc aggatcgtcc
     2881 atgacagtaa aaacggaatc atcggcagct gcattcagtg acgcaaacat gccccccgcc
     2941 aacataacga tggcaggaac tgtcttcaaa agcttcattt atcaagagtc cgtacaacaa
     3001 aaaaagagac catcgcggtc ccggaaactt tcttaaggat caaagattag cgtccctgga
     3061 aaggtaacga attataaaa
//
LOCUS       DAZGJW010000307         3077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZGJW010000307 DAZGJW010000000
VERSION     DAZGJW010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.310"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1164
                     /gene="iha"
                     /locus_tag="ACHKIK_005159"
     CDS             <1..1164
                     /gene="iha"
                     /locus_tag="ACHKIK_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO9086113.1"
                     /translation="ENKGRELVRNVLKRDKWGLAGQLRELKESNLILNSLLLTPLGES
                     HLVTVGGEYQSSSMKDGVVLASTGETFRQKSWSVFAEDEWHLTDALALTAGSRYEHHE
                     QFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLHKGISGVSGQGKTNLLGNPD
                     LKPEESVSYEAGVYYDNPAGLNANITGFMTDFSNKIVSYSINDNTNSYINSGKARLHG
                     VEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSYTPEHMVNAKLNWQITEEVA
                     SWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAWTVVDAGLSWKVTDALTLNA
                     AVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYVIPERNYWMSLNYQF"
     gene            complement(1418..1775)
                     /locus_tag="ACHKIK_005160"
                     /pseudo
     CDS             complement(1418..1775)
                     /locus_tag="ACHKIK_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            1995..2759
                     /locus_tag="ACHKIK_005161"
                     /pseudo
     CDS             1995..2759
                     /locus_tag="ACHKIK_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          777 a          779 c          790 g          731 t
ORIGIN      
        1 gaaaataaag gtcgtgagct tgtacgcaat gtactgaaac gcgacaaatg gggccttgcc
       61 ggtcaactgc gggagcttaa ggaatctaac cttatcctga attcattgct gcttacccct
      121 ctgggagaat ctcatctggt tactgtgggg ggcgagtatc agagttcgtc catgaaagat
      181 ggggttgtcc ttgccagcac aggtgaaact ttccggcaga aaagctggtc ggtatttgct
      241 gaggacgagt ggcatctcac ggatgcactt gcgctgactg cgggcagccg ctatgaacat
      301 catgagcaat tcggggggca cttcagtccg cgtgcatatc tggtctggga tgtggcagat
      361 gcctggacgc tgaaaggcgg tgtgaccacg ggatataagg cacccagaat ggggcagctc
      421 cataaaggga ttagtggtgt gtccgggcag ggaaaaacaa atctacttgg taaccccgac
      481 ctgaagccgg aagagagcgt cagttatgag gctggggtgt attacgataa ccccgccggt
      541 ctgaacgcca atatcacagg ttttatgact gacttctcta acaagattgt ctcttattcc
      601 ataaatgata acaccaatag ctatataaac agcggaaagg cccggttgca cggtgtggaa
      661 tttgccggca cattgccgct atggtcagag gatgtcacgc tgtcgctgaa ttacacctgg
      721 acccgaagtg aacaacgtga tggtgataat aaaggtgcac cgctgagtta taccccagaa
      781 cacatggtga atgcgaaact gaactggcag atcaccgaag aggtggcatc atggctgggt
      841 gcccgttatc gcgggaaaac accacgtttc acccagaatt attcgtcgct gagcgctgta
      901 cagaagaaag tatatgatga gaaaggagaa tacctgaaag cctggacggt ggtggatgca
      961 ggtctgtcgt ggaaggtgac ggatgccctg acgctgaatg cggcagtgaa taacctgctc
     1021 aacaaggatt acagtgacgt gagtctgtac agtgccggta agagtacgct ttatgccggt
     1081 gattacttcc agacgggatc atcaacaacg ggatatgtga tacctgagcg aaattactgg
     1141 atgtcgctga actatcagtt ctgataacaa caaaacgcta tcactgacgg tagcatacgt
     1201 tgccactgca actcctggcg gaacggtggc aatgttttag gttaagtgcg tttccggtcc
     1261 gctaatgaga tttcgttacc aacaactaat atcatcaccg gaaatgcacg gattattttt
     1321 taactcatca tttacatact tgtccagaaa tttatactcg gctagctctg aattaagggc
     1381 cgcgtccgca tctccattac cgtcttatat cactggcgct gacaattgtc tcacatatag
     1441 ctgcagtatc ggctccatcc ccgtggctac gcgctgaaat tctagtgaac cgctttcgtg
     1501 gacttgagtg aaccaactgt ttagctgtga tttatttggg gggcagaata cggtagaact
     1561 cgagtgccag ttgctgcgct attttctgtt ccggttctgt ttcacaaatc gggacgcata
     1621 attttcttcc ccctgattat tttccagggc accaccccgc agcttcccag ggacagtgaa
     1681 ggaagtcggg cggcaatagc aaacggcggg atccaacctt tacgccattt ggcaacagta
     1741 tctctgatga tagtttcagt gcctgtaact ccccgttact ttattcgtac ccttgtaatg
     1801 gggtgttagc cagccccggc atgattaatt cccccagtcg tccatgattc ggggggatgt
     1861 caccgggtct ggtggggcgc tggtaaccgc tgataggggc ctggtcagac atcatgtcgg
     1921 gacagtctgt aacgtggatg ccggtacctg ctccccaagg ttatctggtt aacccgtata
     1981 aacggggaga cacaatgccc gaatccagcg attacgaatc aatcctggtc tttattggcg
     2041 ttgatgtagg taaagacacc cattacgccg ttgccgttaa tcgttcaggt aaacgtttgt
     2101 tcgacagagt attacccaac gacgaaaaca aactcagagc gctgatatct gacctgaaac
     2161 agcatggaca gatactgctg gtcgttgacc agcctgctac catcagggcg ttacccgtcg
     2221 ccgttgcccg ctctgaaggc gtccttgtcg gataccttcc tggactggca atgcgacgca
     2281 tcgccgattt gcactccggt gaagccaaaa ccgatgcccg tgatgcggcc atcattgccg
     2341 aagctgcacg tactctgcct catgctcttc gctctctaaa actggccgac gaacaaatcg
     2401 ccgaactctc catgctttgc ggcttcgatg atgatcttgc cgcacagaca acacaggcca
     2461 gcaaccgcat ccgcggcttg ttgacccaga tacatccggc tctggagcgc gttctcggcc
     2521 cgcgactgga tcatcctgca gtacttgatc ttctccagcg atatccctca cccgaaaaac
     2581 tcgctttact gggtgagaaa aaactggcaa cccagctctg caaactcgcg ccccgtctgg
     2641 gaaaacgcct tgctgcagat atcccccagg cactgacaca gcaaaccgtc atcgtccccg
     2701 gtacgaatgc tgctgtcgtt gttctcccac gcctggcact ccagctcatc acgctgcgtt
     2761 agccaagaga cgaagtgact ggcttcaact ataagtttgt taataccagt gacgctgatg
     2821 tcgttacagg cgatagcaca ggacgcaaga cgatggtaac ggtaatagag aggcagatga
     2881 accatccact ggaagattgc ggacctctct taaaattagt acattacgga ccgccacgct
     2941 ggaaagacag catatggaaa ggctccctgt caaacatttt gtttaacccg gaccctgacc
     3001 atctaacctt ggcaagtgga agattagcgc ctctttgaaa aaggatcaaa cacaacacag
     3061 cagaaaacga ttatagt
//
LOCUS       DAZGJW010000308         3032 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZGJW010000308 DAZGJW010000000
VERSION     DAZGJW010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.311"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(198..800)
                     /locus_tag="ACHKIK_005162"
     CDS             complement(198..800)
                     /locus_tag="ACHKIK_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086114.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(887..1093)
                     /locus_tag="ACHKIK_005163"
     CDS             complement(887..1093)
                     /locus_tag="ACHKIK_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9086115.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1845..1994
                     /locus_tag="ACHKIK_005164"
     CDS             1845..1994
                     /locus_tag="ACHKIK_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015704109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO9086116.1"
                     /translation="MEKVISHTRYKLTPAEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVI"
     gene            2806..>3032
                     /locus_tag="ACHKIK_005165"
     CDS             2806..>3032
                     /locus_tag="ACHKIK_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9086117.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPT"
BASE COUNT          784 a          729 c          810 g          709 t
ORIGIN      
        1 tgatgaatca acaggcatat aactgatacc cgccggtcag aagttccagc ctgcgacttt
       61 cctgtctgcg tgcccgcagt ccggcgttca ggtgcagcgt gtgacagaac gcacggagag
      121 cgaaacggca ggcagacagg ccgttcagtc agccctggca ccctgcccgg ataccagctt
      181 ccctgaaaca actggcatca ggtgcgctta tcgtcaaccg ttgtgcggta tgccaggagc
      241 acgtactgat ccatcagatc cgtcagtgct gcgctctgct tctgacggtt gatgatccat
      301 tcctccagct ctttttctga tttccgtttc agattctccg tccagtcgtc aaggcagttt
      361 ccggtcatta aatcaagcca gcaaagccag accagtttga ggcgcaactc cgtgccacca
      421 aactgacgga agaggctgat cagtttattt gccagcgaac gctgcctcag acgttcacat
      481 tcagacagga gaatgccttc cagcatcgga tgatgaacca gcagatgttc actggtgcat
      541 aaattcacta tcagatcatc atccaggggc ggcgtggtcc gtttgtccgg accataaatc
      601 agccggtgca gactctgggg tacgatggca atctccggtt tttcaccatt gaatgacacc
      661 cacccgggcg tcagattatg gaagtagtag atattcggat gacacacgcc ccaggatacg
      721 gcgtgccggg tgaagccggt cagccatttc tggtagcttt cgtggagcag ctcgtaacgg
      781 gtctgatact gtggtttcat tttcatattc ctcacggggg ataacaggca gcagaacgcc
      841 gccggcaggc cggagactgc gtctgctgca gatgaacggt taagaattac ggacgggact
      901 gtgcaatacg cgcctgcagc caggcttcca cttcgctttt cagccatcgg gagctgcggc
      961 ccattttgat gggcgcagga aagtcaccat ccttgatgag tttgtaaaac cacttatcgg
     1021 tcaggccggt cagttgagtg ataaatgcca tgtcgaccat ctggtcatcc atcagtgaaa
     1081 ctggggtgat catgagaatg ttcctccgga tgttgttaaa ccagccggtg tggtcatgaa
     1141 aacaacgcac caccttcacc ggcataacaa ccggggagaa acgtaaaaaa taatcgggaa
     1201 tgtgaccgcc aggagataaa cggggaggtt acggtggctg agccgtaccg gtatgccggg
     1261 tggttgttac gaaacgatgc ttccgcacgc gcgcgcgata aagaagggat gattttcgtg
     1321 aggaagaggg gaaaactggc gttctgataa ttgcagagtt atttacgggg cggttacgat
     1381 ggggcagacc gtgtccggtg ggcaggcgac aggtatcacc gatgggataa gactgtctga
     1441 ttggtattat aaatatataa gagagatcaa acacatgaga tcaaatacat gagttcatat
     1501 atgtgaaata aacacggtga gtgtgatcag tgggcatgat gagaacgcag tagcaacaga
     1561 ctgcgctatc cctgatagct ggcgtataac ctgcgtatat aaaacgtacc agcaacgcac
     1621 gtgaacagag tgcattaagc atccgtcgtt gacaccgacc tggtgagtga cagtctgaga
     1681 gaccggttaa gtggtggtac gggagtaatg agtttgggga gggtaccaga atgtcagtct
     1741 ggtacggtat gctgacagta tcacgttaat tgcagcataa agtgatgaga ggctaatgga
     1801 agttaaaacc agagcagttc cgtcgttgtt catcccggga gacactggaa aaagtgattt
     1861 cccacacgcg ttataagctt actccagcgg agatggaagc cttcaactct gcggtcgatc
     1921 accgactggc agaactgaca atgaacaagc tttacgatcg tgttcccgct tcagtctgga
     1981 aatatgtcat ctgagcgttc ggtaatcaca actgcatcgc gctaggtgtt atccgctttg
     2041 tgattcttat tggctcttct caggttatta aaagctgaat atgttcctgt tgtcgggaac
     2101 caggataata ccgggagcgt taagcggtat agcgacaaca aagaattcga aatcaaaagg
     2161 gaatggtgcc ggaggcgtgg gcggtaatag gctgattttc aggtattacc ggcttacaac
     2221 gtgtggttga tattgagggg ctgtgagttt cgcgtatttg tcagaaaccc agatgagttt
     2281 tcctgtatgg gacgaaataa ggcagcggca atacctggaa aatttaatat ggtatgaagg
     2341 agaacctgtc ggttacgctt aatatacacg gtacactgtc agctcagggg atacatgaac
     2401 cgttatctca ccagctacac agtatacaca cactgacgct gacagtagtc agcaggacca
     2461 caataatcag cgccagcagt atggtaacga tacggtctgt attccggtag tagccgaagg
     2521 ggtcgacaga accacaatac gggcaacaca ctgtattatt agtaatcgcg tttccacagt
     2581 ccctgcactt cttttgcgta tcaccataac tccctcctca cagccttacc cgtaaccggt
     2641 tttttacatt aaaaaacaac gctgaaaatt caaccgtcag tccggagacg accgttcggg
     2701 ttatcacaga gtgcctgaga cagtgtcctg ccggaggtca cttcagggac tctgcgcatt
     2761 ttttacgacg tggctattcc gtatgaacca tacggagatt taaccatgac ctacaaatac
     2821 aaccccttct ggcagcaacg tattcgtgag acggtgcggc acgcactgga tgttcatccc
     2881 cgtctgacgg cattgcgggt tgatctgcgt ctcccggatg taccggcagc aacggacgca
     2941 gctgtgatat cccgcttcat caatgccctg aaagcccgaa tcgacgctta ccagaaacgt
     3001 aagcatcggg aaggtaaacg cgtgcatccc ac
//
LOCUS       DAZGJW010000309         2997 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZGJW010000309 DAZGJW010000000
VERSION     DAZGJW010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.312"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(136..1890)
                     /locus_tag="ACHKIK_005166"
     CDS             complement(136..1890)
                     /locus_tag="ACHKIK_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HFO9086118.1"
                     /translation="MRNKNIIMLLMSSLILSGCGPESENKESQQQQPSTPTDQQVLAA
                     QQAAIKEAEQRSVADKATADAKAKALAQQEAQQYSDKQALQGRLQAAPKYQHAAREKA
                     ASQIANPGTARYKQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNHGLLPPPDAV
                     RVEEIVNYFPYDWDIKDKQSIPATKPIPFAMRYELAPAPWNEQLTLLKIDILAKDHKS
                     EELPASNLVFLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALP
                     SISGSHKAEINAAIDSLDAEGSTNGGAGLELAYQQAAKGFIKGGINRILLATDGDFNV
                     GIDDPKSIESMVKKQRESGVTLSTFGVGNSNYNEAMMVRIADVGNGNYSYIDTLSEAQ
                     KVLNSEMRQTLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIG
                     AGKHITLLFELTLNGQKASIDKLRYAPDNKSVKSDKTKELAWLKIRWKYPQGKESQLV
                     EFPLGPTINAPSEDMRFCAAVAAYGQKLRGSEYLNNTSWQQIKQWAQQAKGEDPQGYR
                     AEFIRLIELADGVTDISQ"
     gene            2028..>2997
                     /locus_tag="ACHKIK_005167"
     CDS             2028..>2997
                     /locus_tag="ACHKIK_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFO9086119.1"
                     /translation="MKKINFAFIILFLFSLPLIIFYQPWVNALPPMPRHANPEQLEKT
                     VRYLTQTVHPRSADNIDNLNRSAEYIKEVFVSSGARITSQDVPITGGPYKNIVADYGP
                     ADGPLIIIGAHYDSASSYENDELTYTPGADDNASGVAGLLELARLLHQQVPKTGVQLV
                     AYASEEPPFFRSDEMGSAVHAASLERPVKLMIALEMIGYYDSAPGSQDYPYPAMSWLY
                     PDRGDFIAVVGRMQDINAVRQVKATLLSSRYLSVYSMNAPGFIPGIDFSDHLNYWQHD
                     IPAVMITDTAFYRNKQYHLPGDTADRLNYQKMAQVVDGVINLLYNSK"
BASE COUNT          773 a          721 c          639 g          864 t
ORIGIN      
        1 atacacaatt agaaataaaa cactcacaac ccaaaaatag agcatccggc aaaagagtga
       61 gttaacatca ttgttaatca attcgaccag tcagcaaata tgctgactgg tcgaacatag
      121 tttgctaaac agtcatcact gactgatgtc agtcacacca tccgccagtt caatcaggcg
      181 aataaattcc gccctgtaac cctgtggatc ttcccctttt gcctgctgag cccactgttt
      241 gatctgctgc caggaggtat tgttcaggta ttcagaaccg cgtaactttt gcccatatgc
      301 agctactgct gcgcaaaaac gcatatcttc agagggggcg tttattgttg gccccagcgg
      361 gaattcaact aactgacttt cttttccctg cgggtatttc cagcgaatct ttaaccaggc
      421 cagttctttc gttttgtccg atttcactga tttgttatcc ggggcatagc gtaacttatc
      481 aattgatgct ttttgcccgt tcagcgttaa ttcgaataac aacgttatat gtttgcctgc
      541 gccaatatcc cccgcatcaa cgttgtcgtt attaaaatct tccgcccgaa gttggcgctt
      601 ttcataacca atctgacggt attccgttac ccacgcgggg ttaaactcaa tttgcgcttt
      661 gacatctttt gctacggtaa tcaacgtctg ccgcatttca ctattcaata ctttctgcgc
      721 ttcagagagg gtatcaatgt agctgtagtt gccgttacca acatcggcaa ttcgcaccat
      781 cattgcctcg ttgtaattgc tattccccac gccaaacgtc gacagagtaa caccagactc
      841 ccgctgtttt ttgaccattg attcaatcga ttttggatcg tcaatgccaa cgttaaagtc
      901 accgtcagtg gctaataaaa tgcgattgat gccgccctta ataaacccct tcgccgcctg
      961 ctgataagcc agttccagcc cggcaccgcc attggtactg ccttcggcat ccagcgaatc
     1021 aattgcggca ttaatttccg ccttgtgact accggagata gaaggcaacg caatacggga
     1081 gtcgccagcg taggtcacga tggcaatgtt atcctgctca cgaagttctt tgaccaataa
     1141 tttcaacgaa gactggataa gtggcaaacg ttcatcagaa atcattgaac cagaagtgtc
     1201 gataagaaag accagattag aagctggtaa ctcttcactt ttatgatctt tcgccaggat
     1261 atcaattttc agcaatgtta gctgttcatt ccacggcgca ggagccagtt cgtagcgcat
     1321 agcgaaaggt attggcttag tggccggaat agattgtttg tctttgatat cccagtcata
     1381 cgggaaataa ttgactatct cctccacccg cacagcgtct ggcggaggta acagcccatg
     1441 attgagaaaa cggcgtacat tcgcataact gccggtgtcg acgtcaaggc taaacgtcgc
     1501 caacgggttt tgcgctacct gcttaaccgg attatcatcg aactgcttgt agcgagcggt
     1561 tcctggattt gcgatttggg acgctgcttt ttctctggct gcatgttgat attttggagc
     1621 cgcctgtaag cgcccctgca aagcctgttt gtctgaatat tgttgcgctt cttgctgggc
     1681 taaggctttt gctttcgcgt ctgcagtggc tttatcggcg acggatcttt gctcggcctc
     1741 ttttattgca gcttgttgcg cggcaagcac ttgctggtct gtgggggtac tgggctgttg
     1801 ttgctgactt tccttattct cagattccgg cccgcatcct gacaaaatca aactactcat
     1861 aagcaacatg attatatttt tatttcgcat ataaaatcct tttaaataaa tacaggcacc
     1921 agacataaaa tatactcttt gtataatcct gctaatctta ataagatgca atatcgaaac
     1981 ccataacaaa taatgattca ataacgaaac ccattaataa tttgcatatg aaaaaaataa
     2041 attttgcttt tattatatta tttctgtttt cactccctct tattattttt taccaaccct
     2101 gggtcaatgc attaccgcca atgccccgac acgcaaatcc tgagcaatta gaaaaaacag
     2161 tgcgttatct tacacaaact gtgcatccac gtagtgccga caatattgat aatctgaata
     2221 ggtccgcaga gtatataaaa gaggtcttcg tcagtagcgg tgccagaatt acctcgcagg
     2281 acgtccccat tacgggtggc ccctacaaaa acattgttgc tgattatggt cctgccgatg
     2341 gaccgctgat tattattggt gcgcactatg actctgccag cagttatgaa aacgatgaat
     2401 tgacctatac acctggcgcg gatgataacg ccagcggcgt ggcaggatta ctcgaactgg
     2461 cacgtttgtt acatcagcaa gtaccgaaaa caggcgtgca gctggtcgcc tatgcgtcgg
     2521 aagaaccgcc cttctttcgt agcgatgaaa tggggagcgc ggtacatgca gcttcgcttg
     2581 agcgtccagt aaaattaatg atagcactgg agatgattgg ctattacgac tctgcgcctg
     2641 gtagccagga ttacccttac ccggcaatgt cctggcttta tcccgatcgg ggagacttta
     2701 ttgccgtggt cggcagaatg caggatatca acgccgttcg tcaggtaaaa gcgacattgt
     2761 tatcatcccg gtatttatct gtttattcta tgaatgcccc agggtttatt cccggtattg
     2821 atttctctga tcaccttaat tattggcaac acgatattcc cgccgtaatg attactgaca
     2881 ccgcttttta tcgtaataaa caataccact tgcccggtga taccgcagac agattgaatt
     2941 atcagaaaat ggctcaggtg gtggatggtg ttataaattt gttatacaac agtaaat
//
LOCUS       DAZGJW010000310         2993 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZGJW010000310 DAZGJW010000000
VERSION     DAZGJW010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.313"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(116..2377)
                     /gene="katE"
                     /locus_tag="ACHKIK_005168"
     CDS             complement(116..2377)
                     /gene="katE"
                     /locus_tag="ACHKIK_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO9086120.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            2560..2823
                     /gene="cedA"
                     /locus_tag="ACHKIK_005169"
     CDS             2560..2823
                     /gene="cedA"
                     /locus_tag="ACHKIK_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO9086121.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT          719 a          724 c          768 g          782 t
ORIGIN      
        1 caatacattg aatttatacg actttgtagg ccggataagg cgttcacgcc gcatccggca
       61 taaacaaagc gcactatgta aatcatttga ggcggcgcaa ttgcgccgcc tcccatcagg
      121 caggaatttt gtcaatctta ggaatgcgtg accacacgcg gtgtgctgcc atcagcgtta
      181 gcagttcatc cataaaacta ccgtcagcgc tgtcagcttc cacaatccct tcttcaccct
      241 ggtcagcgac cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt
      301 taaggtgttt gtaggcttcc atcaggtagt agttggcatc gccgttgtca gcgatatccg
      361 cgatattgcc gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg
      421 tagcggctat aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga
      481 gcagtttggc atgaacgcct ttggccttca gggccttgag aatggccaga aggtctgccg
      541 atctcacttc atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa
      601 tggcgtacaa acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga
      661 tattcagctg gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag
      721 tgagatcaat atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt
      781 tgcttaactc aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta
      841 gccagaacag acgcggatgg gaataatatt cgccaaacga tggactgcgc tcacgaactt
      901 tatttccttc cacgcgttcc tgatatgatt caaaaccgcc gcgtttcggt cccggcggtg
      961 tttcgcgcgg ccagttatcg ttgatcgagt tcggttcgta attcgccggg ttagtgtcga
     1021 tccccatacg atgcatgcca tcacgctgga agttgtggta agggcaggtc ggacggttaa
     1081 tcggaatctc atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga
     1141 acaaacgtcc ctgcaacagc ggatcgttgg tgaagtccag accaggaaca atatgacctg
     1201 gatggaatgc tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca
     1261 ttttgccgac acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa
     1321 gatcgaagtc gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt
     1381 ccggaaaatc gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac
     1441 gtccggtgag tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc
     1501 agtggaaacg tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac
     1561 cgaagccttc catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca
     1621 cgttgtgcag agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt
     1681 gcccttgtgg aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt
     1741 tatgcgcatc ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct
     1801 cttcagtata gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag
     1861 cgccaccctg aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga
     1921 ggaaatccgc tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg
     1981 ctgatccgcg tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt
     2041 tctcgcgcag aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt
     2101 tttgatcatc ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt
     2161 tgcgtacgtc ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc
     2221 caggggcggt aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt
     2281 cctcaggtgc cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact
     2341 ggtgctgatg tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt
     2401 actgaaaggg ccgctgtttt ggtcaaaaaa ccctctaact atagaccatc agccagatcc
     2461 cgcttcggag actattctgg aaacggtgtt atgaaataca cgcaaagtgg gacgtacagg
     2521 ggctaaatcg gctaaactac agtttttctg atttttaata tgcgtttagt gaagccagta
     2581 atgaagaaac cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa
     2641 cccgcgccgc cagaacatgc gataaagatg gattcttttc gtgatgtctg gatgctgcgt
     2701 ggcaaatatg ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact
     2761 gtgcctgaat ccgcccagcg ttgggcaaat cagatccgcc aggaagggga agtgactgag
     2821 taaagattac gtacctcccg gttttgccgg gagttacatg acctgaaatt ctgtatttca
     2881 ttgctacatt cattcaaacc accagtaaca aaaagctgcc tttgacgatc tattgctata
     2941 aataagtgat cttttttctt taattgttta tttattttaa aatggttaag ttt
//
LOCUS       DAZGJW010000311         2968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZGJW010000311 DAZGJW010000000
VERSION     DAZGJW010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.314"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            6..149
                     /locus_tag="ACHKIK_005170"
                     /pseudo
     CDS             6..149
                     /locus_tag="ACHKIK_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            153..344
                     /locus_tag="ACHKIK_005171"
                     /pseudo
     CDS             153..344
                     /locus_tag="ACHKIK_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin"
     gene            complement(352..705)
                     /gene="cmi"
                     /locus_tag="ACHKIK_005172"
     CDS             complement(352..705)
                     /gene="cmi"
                     /locus_tag="ACHKIK_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HFO9086122.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GIYHCEEGYVEYAND"
     gene            complement(878..1660)
                     /locus_tag="ACHKIK_005173"
     CDS             complement(878..1660)
                     /locus_tag="ACHKIK_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO9086123.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNKHA"
     gene            complement(1662..2075)
                     /locus_tag="ACHKIK_005174"
     CDS             complement(1662..2075)
                     /locus_tag="ACHKIK_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086124.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELIFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            2635..2865
                     /gene="vapB"
                     /locus_tag="ACHKIK_005175"
     CDS             2635..2865
                     /gene="vapB"
                     /locus_tag="ACHKIK_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HFO9086125.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLEYEKADPDFMAEREDVVSDEGRFNL"
     gene            2862..>2968
                     /locus_tag="ACHKIK_005176"
     CDS             2862..>2968
                     /locus_tag="ACHKIK_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
                     /protein_id="HFO9086126.1"
                     /translation="MNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHR"
BASE COUNT          761 a          750 c          698 g          759 t
ORIGIN      
        1 tatctatgtt atttaaacaa gctatgatac agtttttatc tttaaaaaga gttaatggaa
       61 tggatagtat taaattactt gataaagtta ttatctatca acaacagaat gagcaattat
      121 atccagcaca ggaacatctt cttttacaat gttcatatga tgataaatac gattttaacg
      181 ccagcactca ccgtggcatc atcggagagt cgctcacaag gctcggggcg atgttttctg
      241 gtaaagagta ccagatactg cttcctggtg aaattcacat taaagaaagt ggtaagcgag
      301 aatataaggg ccgggattcc ggccttttta tgcagccttt ataacagtgt attagtcatt
      361 cgcatattca acatatccct cctcacaatg gtatatgcca taatacattc tcttaccctc
      421 cgggccttca gctttaaatg ggacaagaag tgttttttca tcttcaacat ctgttaaact
      481 tctctcaatc ttttcccatg taattttcgg gatttctgta ccaagaagtt gaacatcgtt
      541 gtcccagcgt ttcattcttt gcaggtaatc attttttaca aacgttctgg cctgttcatc
      601 gctgacctga tagcatgccg gacctttatc ttcagaccag aaaaaaacag cagcaagagc
      661 aataatcgtt aaaataaaaa taaatttcat gctaattact ttcataaaat ctccacaact
      721 tctgtcgttc attcgatata aaaaaacatt acgaatatat ccgtacaatg tgagcatact
      781 gtgaagtggc tttaacaatt atgaaataat acaagagatc acatattgca gtggtctctt
      841 ttttatagaa gaaatcacaa ttcataattg tgacttatta tgcatgtttg tttttcagca
      901 ttgtgaccgc atcggactca ggcatcgaaa actgcacccg gtgccgggca gcgacatcca
      961 gcgcaaacac cttcgtgtag acctccgttg agctgatgga cttatgcccc atcagactct
     1021 gcaggacctt cagcggtata ccggcataca gcatatgcat cgcataggaa tggcggaacg
     1081 tatgtggcgt gaccgggaca gagaacgtca caccatcagc ggcagcggcg gcaaccgcct
     1141 ccccaatcca ggtcctgacc gttctgtccg tcacttccca gatccgcgct ttctctgtcc
     1201 tgcctgttcg tttattacgc cgttccatgg ggattttcaa tgtggccacc atcgtctgca
     1261 gctggctgac gtaccaggag tcggagagcg gaaccagccg gtgagtctgc tgcccggcgg
     1321 gcgttcttcc tgccgtcctg gcggcttttt cggtccgttg tttcagggtc gcaagctgca
     1381 caaacggata cggaggcgtg agcgaaaaat ccccccgcgt cagcgccagt gcttcattaa
     1441 tgcgcgcacc ggtgttccac agtgtggcca gcagcatctt gcggcgcaga tccgggacgt
     1501 aatggagcag ggcgctcact tcaggggcca gcaggtattt gggcagttca tcatgaacca
     1561 tcgacatctg gcggagtgcc agcgctgccg gataatcgat ggcaacagga agctgagcgg
     1621 catcccgggc atggtggatc ggtgccggca ggtgctgcat atcagtgttc actccacggg
     1681 gaaacgtcgg gcaggttttc cacaacccgg tactttcccg gataacggag ctggttaccg
     1741 ccacgcgtcc agacggtgac aacaaaaccc cctccctggt tgttttcggg gagcaggcga
     1801 tcgcactgct cactcagcat gtggatcacg aacggcgcat cgctgccgtc ttcaaatatc
     1861 agttccagcg catcgcggcc atgcagcgtt cccttactga tgatgacgta ctcagccccc
     1921 cgcatctccc tgagtaaatg ttttgccgca tccgggacaa gcagcctggc tgcgccggca
     1981 ttccagctga ggtagacata tccggcctgc gcctgtacag actgccagta atccgttctg
     2041 gcgacagcct gaccttcatt ctcaataaaa aacatgggtg actccttttt tcttttccgg
     2101 gcagggacgg tcatgcgata acgtcatcca tcatcactgc tcttaccgtc acgccatgtg
     2161 ataacatcat gccgttatca tcatcaccat gataacaagc atccatataa ccacgctgtt
     2221 aaattaccgg aggaccattt ccgacagatc cgatttttct gttattgacg ggttttcgga
     2281 agtgtatcac gctgaggaaa taatcatatc gctgttttcc gcccctgcgc gacgcatcac
     2341 aggtcaggga gatgacacaa catatagtgt catcagcaga tgaatccggc accacatgtt
     2401 gtgatgacgg gaggtggcgt tgtgtggggg gagaggaaga tcctgaacaa ccttttttcg
     2461 gatctccgat aaaaaagaaa gaaatagaga tatgctaaac atatgcctgg catctacata
     2521 tgctaccgct atacatatgc cacacatctg cctatactaa acatatgcac acacccggtg
     2581 ctttgaatat tccggatatg tttcacatat ccatatgctc gggagagaaa gaacatgaga
     2641 accgtatcga tatttaaaaa tggcaacaac cgcgccatcc gtctgcctcg tgatctggat
     2701 tttgaggggg tgagcgagct cgagatcgtc cgggaagggg acagcatcat cctgcgcccc
     2761 gtccggccaa cctggggatc attcctagag tacgaaaaag ccgatccaga ttttatggcg
     2821 gagcgcgagg atgttgtcag cgacgaaggg cgatttaacc tgtgaacaag acttatatgc
     2881 ttgatacctg tatctgctcg ttcatcatgc gtgaacagcc agaagccgtc ctgaagcgtc
     2941 tggagcaggc ggtgctacgc ggccaccg
//
LOCUS       DAZGJW010000312         2960 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZGJW010000312 DAZGJW010000000
VERSION     DAZGJW010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.315"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(35..892)
                     /locus_tag="ACHKIK_005177"
     CDS             complement(35..892)
                     /locus_tag="ACHKIK_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9086127.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            1141..2010
                     /locus_tag="ACHKIK_005178"
     CDS             1141..2010
                     /locus_tag="ACHKIK_005178"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO9086128.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(2170..2763)
                     /gene="rclC"
                     /locus_tag="ACHKIK_005179"
     CDS             complement(2170..2763)
                     /gene="rclC"
                     /locus_tag="ACHKIK_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO9086129.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(2775..>2960)
                     /gene="rclB"
                     /locus_tag="ACHKIK_005180"
     CDS             complement(2775..>2960)
                     /gene="rclB"
                     /locus_tag="ACHKIK_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO9086130.1"
                     /translation="FSTNADDKIILKHISVSSVSASPTVLEDTIADIARKYNASSWKV
                     TSMRIDNNSTATAVLYK"
BASE COUNT          850 a          607 c          657 g          846 t
ORIGIN      
        1 gtctcgttat aaattcattt agatatagcg aaaactaaac cggacgaacg gggataaaaa
       61 tttcgcaaac aatgttattg ctatcaattt cgacgaaatt ttcattgtaa taaaaccgct
      121 caatatcact gccgttcact ctggctaacc ccctggcggg taattccatc agatagaggt
      181 ttcgcgccca ttcagggtag tgctcccact caccttcata gcggaaagag gcatacagtc
      241 ctcggttaat tgtccactct ttcggcatct ctgaatcggt ttgtttgcat accgtaccgg
      301 caaaagtttc aaccgtgagc aaatcgaccg attttgttga cggttcaaac gtggagacga
      361 aatagatatc cgtcttaaaa ttattcatac atctggctaa atgatgacgt cgcagtttga
      421 cttcttcatc cggtgattta ttcgacactt caatatcata tttaaatgaa ttgccaaaaa
      481 taggtgtctc aggaaagttg actaatctac actcggtctt ttgctgttga cgtattaaaa
      541 aagaagggaa gatatttgcg agatcccaat aatcacggtg gcggtataca cggggagagc
      601 agccgaataa ctttttaaat tcgcggctga atgactgctg tgaatcaaaa tgcaaagaga
      661 gtgcaatatc aagcatagat ttcgcggtga gccggacaag aatggcggca cgacaaagcc
      721 ttcgtttgcg aatgtattct cccaaaggca catgcatgaa atttcggaac agaagctgga
      781 tgttgcgtct gctgtagcca gatttctgtg cgatatcttc gattgaaata gggtgctcaa
      841 gattgcactc aatccactcc aggagctgct gtagaatctt ctgcctgatc atcacattct
      901 ccattgattc ttcgtcatat cgcacatttt cgttatgccc tgggccctaa gtgtatcaaa
      961 aacagcaagg gcgaaggcaa aagcactggt agcgtctgct tcgttgtccc cgaaaataga
     1021 gaggtttcag tcctgcatta ttaatgaatt ttttgcataa gtgatatcaa aatccacgta
     1081 ctaatttgag gttacgtttt aacgtagact cattgttcat gcctaatgga gggactgaca
     1141 gtggaatttt ccgtattaag taacaatctg aaaatgccga tgatgggatt tggtgttttt
     1201 caggttaccg ataaaaacgt gtgcaaacag tcagtgttga acgctatccg cacaggttat
     1261 cgactcatcg acactgcagc ggtatacggt aatgaagatg ccgtcggtga agccgttcgt
     1321 gaagctatat ctgaaggttt atgtacccga gatgagttat ttataacgtc taaattgtgg
     1381 gtgcaggata tgttgaatca ggatacggca gcagcaggta ttgaagcatc attaaaaaaa
     1441 tcgggactag agtacttcga cctctattta ttgcaccagg ctatgcgcga ttatttcagt
     1501 gcgtggcgtg cactcgaaga tgcctatgaa gagggcaaat taaaagcaat tggggtttcc
     1561 aatttctatc ctcatgttct ggcgaacttt tgtgaaacgg taagagttaa accgatggtc
     1621 aaccaggtcg agttgcatcc ctattttgcc caaccagagg cgctggcaac catgaagtat
     1681 tataacgtgc agcctgaagc atgggctccg ttaggtggtg gacgacataa accctttgaa
     1741 aataatctgc ttcagagtat tgcagatgcc catcaaaaat cgatttctca agtcattctg
     1801 cgttggaata ttcaacgggg agtggtcgtt attccgaaat cgacacatca acagcgtatc
     1861 gaagaaaatt ttgctatctg ggatttctca ctgacagaga aagaaatggc acaaattagt
     1921 tcgcttgatt tgggttatgt tggggagtcg gtaaaacatt ttaatcctga atttgttcgt
     1981 ggttgtcttg ctgtaaaaat acatgattga tattaatcat atattttacc tgagacgaca
     2041 agaatctttt aacaggggag tgatattgat cttcactctg tcatatctcc ggtaatatgg
     2101 cgtcaggctt catcatcaga aagggggcct gacgcctgaa aaagtgaaca acagacagtg
     2161 ttcggattat caatattcag tttttaacgt tgaactggat tcattactgc gttgtttaag
     2221 aatttcccgc gccgaatctg ccattatcat gacagcacct gccagcatca gagtatcttt
     2281 caataccagg cgaccagcac cggataaata agggaaacca tgatgagcgt cacccaatgc
     2341 gggtacccat gcctccgggg tggtgattaa aaatgagagt gttaccagcg gcgtggtaaa
     2401 tgccatcagt cctcccaata aacctaacca gcgattgaca ggattagcca aaaccagcag
     2461 agcaataatc acctccacga cgccaagacc gttggaaaaa ccataggtat tattggccgt
     2521 ttgccatgcc cttgcttctg gtttgtattc gccttcgtga gtcagatact gtttatagtc
     2581 ttccgggtgt tcataaaaga acgacattag tggactgttt gcgacgaatg gtgtaatgct
     2641 gtctgcctcg taagggacaa actttaataa cccaatccac ataaaaacaa ttgcaatact
     2701 cagacgaatc aatgtcaggc caattttatc tccccgactt aacaggtgca ggtatttttc
     2761 cattgtgaac atccttattt atacaatact gctgttgcgg ttgaattatt atcaattcgc
     2821 atcgatgtga ctttccagga tgaagcatta tattttctgg ctatatcagc aatggtatcc
     2881 tccagaactg tcggtgatgc tgatactgac gagacgctga tatgtttcag aattatttta
     2941 tcatctgcat tggtggaaaa
//
LOCUS       DAZGJW010000313         2949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZGJW010000313 DAZGJW010000000
VERSION     DAZGJW010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.316"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1236..1838
                     /gene="fimB"
                     /locus_tag="ACHKIK_005181"
     CDS             1236..1838
                     /gene="fimB"
                     /locus_tag="ACHKIK_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO9086131.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            2316..2912
                     /gene="fimE"
                     /locus_tag="ACHKIK_005182"
     CDS             2316..2912
                     /gene="fimE"
                     /locus_tag="ACHKIK_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO9086132.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          892 a          527 c          604 g          926 t
ORIGIN      
        1 atgtttaatt gatttgaatg ttcgtaagct atatcactta ctcaatccat tttacccaga
       61 gtcataaaat agagtgtctg ttgatctcat tttcatattc ctttcagact gaagtgcatt
      121 ttcatcaccc gatgtgaaag aactgaagtg atgtttgcca tagattgcga gatgccctgt
      181 ctcattgact atcttggtgc acctttatac ctgttatacc agatcaaaaa tcacgcaatc
      241 catacaacaa aaccagattt gcaattcgtg tcacaaaata tgtcgatatt tttctaagaa
      301 gaagatgcca tgtgaagcca aacgaacact tgcggaggcc ttcacaaacc aagggttgtt
      361 atttaattat ctgttttata agtaatttat ttagagtata aacaaggaaa gatttgcaag
      421 ataactcagt gggcgtggtg aaaataagtg tttacccgcc atcaggctga gcataattct
      481 catcatgaaa tatgtttcct ggtttgtggc ttgtagctgg tcactcctga agtctctctg
      541 gagaggcttg ttgatgttgg tgttttcagg atgatgtttc acttagcttg tttgccgaat
      601 agcccggcga acagctgtga ttgaggaagg tttaagtcgc agtgaccaaa gccatattta
      661 ccaacggatg cagatgaaaa aatcatctcc tgctttcccc catatctcca ggataaaaag
      721 gaatgtaaca atctcatgaa ctaaactgac gaaccagcca ggaataatcg ctagggacct
      781 aagaattagc ctgataatag ctactaagaa attactgcgt tccatgaaat agccattttg
      841 tggcaaatgg agttgtctaa taatgtcata tgtgagacgg atagttgaac gaatattaaa
      901 ttttgctgaa ttttttatgt tgattttact tgttacagaa catatcacat gatatataga
      961 taagattagt tgcattaatg atgagggtta ttattagatt cgtatccgat tgataaatat
     1021 ataaaggtac atagcatgca agagcatagc gtttgtatgg caacgttatt ataagtaact
     1081 gttgctactc catttaagtt cactcagaag aactggtcca cttacgttag ttattaagca
     1141 aacgttcgct tttataaaca taatcaggat aaaaatgttg gattattgct aacccagcac
     1201 agctagtgcg cgtctgtaat tataagggaa aaacgatgaa gaataaggct gataacaaaa
     1261 aaaggaactt cctgacccat agtgaaatcg aatcactcct taaagcagca aataccgggc
     1321 ctcatgcagc acgtaattat tgtctgactt tgctttgttt tattcatggt ttccgggcga
     1381 gtgaaatttg tcgattgagg atttcggata ttgatcttaa ggcaaagtgt atatatatcc
     1441 atcgattaaa aaaaggcttt tcaacaacgc acccgctatt gaacaaagaa gttcaggctt
     1501 taaaaaactg gttgagtatc cgtacttcgt acccgcatgc tgagagcgaa tgggtatttt
     1561 tatcgcgtaa gggaaatccg ctttctcggc aacagtttta ccatattatc tcgacttccg
     1621 gtggtaatgc cgggttgtca ctggagattc atccgcacat gttacgccat tcgtgtggtt
     1681 ttgctttggc gaatatggga atagatacgc gacttatcca ggattatctt gggcatcgca
     1741 atattcgtca tactgtctgg tataccgcca gcaatgcagg gcgtttttac ggcatctggg
     1801 atagagccag aggacgacag cgtcacgctg ttttatagag atagttgaga taccagggat
     1861 ggtgttaaaa actacattat tcttacgata tatcaaaaat gattaaccat ttattaattg
     1921 atgttattgt aagtgtgatt attaattttc ataataaaca ttaagttaac catatccata
     1981 caaaatacaa tggtttatgt tcttaaaaat aaataaacaa aatcattcat aaaattacac
     2041 atcacttaaa ttctcctgtt tccgcacttt tttctttatt ttttaagcaa ctggaagtta
     2101 atccactgca atctattgtt atattgaatc aaatcaatga aaatagatgt tatcacatca
     2161 gtgatatttt atttttgtat gatatttaat gtaattgact gatagccaca tcacttcgtg
     2221 tgtggttatc tttttatcta ttgggctaat tttgaccgat tgaggtttcc tataggtatt
     2281 cattcaaata tatctcagtc aggagtacta ctattgtgag taaacgtcgt tatcttaccg
     2341 gtaaagaagt tcaggccatg atgcaggcgg tttgttacgg ggcaacggga gccagagatt
     2401 attgtcttat tctgttggca tatcggcatg ggatgcgtat tagtgaactg cttgatctgc
     2461 attatcagga tcttgacctt aatgaaggtc gaataaatat tcgccgactg aagaacggat
     2521 tttctaccgt tcacccgtta cgttttgatg agcgtgaagc cgtggaacgc tggacccagg
     2581 aacgtgctaa ctggaaaggc gctgaccgga ccgacgctat atttatttct cgccgtggga
     2641 gtcggctttc tcgccagcag gcctatcgca ttattcgcga tgccggtatt gaagctggaa
     2701 ccgtaacgca gactcatcct catatgttaa ggcatgcttg cggttatgaa ctggcggagc
     2761 gtggtgcaga tactcgttta attcaggatt atctcgggca tcgaaatatt cgccatactg
     2821 tacgttatac cgccagtaat gctgctcgtt ttgccggatt atgggaaaga aataatctca
     2881 taaacgaaaa attaaaaaga gaagaggttt gatttaactc attgataata aagttaaaaa
     2941 acaaataaa
//
LOCUS       DAZGJW010000314         2922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZGJW010000314 DAZGJW010000000
VERSION     DAZGJW010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.317"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(280..498)
                     /gene="ymgF"
                     /locus_tag="ACHKIK_005183"
     CDS             complement(280..498)
                     /gene="ymgF"
                     /locus_tag="ACHKIK_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO9086133.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            complement(630..2153)
                     /gene="pdeG"
                     /locus_tag="ACHKIK_005184"
     CDS             complement(630..2153)
                     /gene="pdeG"
                     /locus_tag="ACHKIK_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO9086134.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETNEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(2485..2733)
                     /gene="ymgC"
                     /locus_tag="ACHKIK_005185"
     CDS             complement(2485..2733)
                     /gene="ymgC"
                     /locus_tag="ACHKIK_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO9086135.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            complement(2846..>2922)
                     /gene="ariR"
                     /locus_tag="ACHKIK_005186"
                     /gene_synonym="ymgB"
     CDS             complement(2846..>2922)
                     /gene="ariR"
                     /locus_tag="ACHKIK_005186"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO9086136.1"
                     /translation="SDILKADVIRKTLEIVLRYTADDM"
BASE COUNT          917 a          617 c          580 g          808 t
ORIGIN      
        1 agcgacaata tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga
       61 cattgatcac tttgaggtga cgattttgtt taacaaataa ctcttcgaca tcacaatcat
      121 tgatagttgc aataaaaatt atttcaatga catataaaaa caactgtgat aacaccaggg
      181 ttgacaacta tttgcgcaat aattccttta cttcaatgat ccaggcattc actgccagca
      241 acccattcag ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct
      301 gacgagtctt tcccttaatc gattaaccag attgatcgcc agccaaacaa gggcattaag
      361 caaatttatc gtcacaagca cttctcccag cccggtactg ggttttgcaa taaggatgac
      421 caggagggtg atcagcataa atgcaatgga aaagatgata tagagagtga aataatccag
      481 attattacta ttgttcatgc tactaatggc aatcctgtca aaactttcaa acaattatag
      541 caaacaaaat aatgaacaat agtaacaaga ggcaacgagc taattagctg tctataatta
      601 caccgcacta tggccttact gatcaatttt cactcaacca caaccttcac cttcggttta
      661 gaaaggataa ttttgaccag gtcgatgtat gtaacaggtt tatagaaata ataaccctgc
      721 tgaaatgtga tattattttg gttcagatag tcaagttgtt cattcgtttc gacaccttca
      781 gcgacgatac tgatcgaaag tttacgcgcc agatccaata cacaatccag aattcgggtt
      841 gattctgggt ctgcgtttac gcggccaacg aaactgtgat caattttgat ataatcaata
      901 tgcaggtcat gaagataaga aagccctgag tagccagtac cgaaatcatc cagcgcgatg
      961 acaaaaccat tttcatgcag aatgttcaac cgctgaacca gactttcatc aacattcaat
     1021 ggctcacgct cggtgacttc cagaacaagg tttaaatcgc ggcgggtaaa actgtcacgg
     1081 aaatttaaac actcgtcgac aaacgtcggc gaaataatat gcgaagcgct aaaattaatt
     1141 ccaatatgaa aaccttccgg caatttactc gcgatagcgt tcatctgtct ggcaacctga
     1201 ttcatcaggc tttgcgtaag cggaacgatt aatccggatt tttcagcaag tggaataaat
     1261 gccgcgggtg atatatatcc accgtgaggt tgtttccagc gggctaacac ctcaactccc
     1321 cgcaatgtcc cttcccgacc atttaccaca ggttggtaaa aagggacgat ctccccatta
     1381 tttattgccc gtcgcaggat ctcttcaggc gttgcgcttt tattgaaata tctatcaagc
     1441 aggaaggcag cggcgcaggc gattaacaga ataaatatta aaatcccaaa gcctttatcg
     1501 ataagtctgt agaagctaaa gagtggcggc gggttaaaga taatagtgaa atgatatttt
     1561 gtggagtgaa ccttctcggt atagggataa tgtccattaa gcgtcatcac atcacccgtt
     1621 ggtccaataa tgtcatccgc cacgcgtaat acatacctta cccctttcaa gggtacatta
     1681 agtgccccac gaatatgctg atcgcttatc gtaaccaaaa tgcgcgtatc tgcaaattgg
     1741 ttctgataga gcaggatagg taatctatta acggtgtcga ttgctggagc caacagtaaa
     1801 ttactgtccg ggaaaacagg aatacgagac aacaggaccc gattcccagg cagggatgta
     1861 caccagacta ttccctgttt gagaatgatg attgtgcgca ggtgaggttt cagagctgct
     1921 tcagtgccaa gctgatattg ctcctcgagg ttgcattcct tcccggcaat gttaaccgct
     1981 gtttgagtcg cttgtgacgc ttcttcaagt atatgattga tattgttggc ggcatatctc
     2041 gctcccgcca ggtactctgc ttttgcggaa taccagagct ggatgttcaa aatagcgaca
     2101 ccggtaataa acaagcatat cgcaacgagg atgggtatga gtgtattgcg catttgcttg
     2161 tcccggccaa agggtttaat acatgaaaat gccattcatt cgctcgcgct ggcatctaca
     2221 gagagcatgg tagagagcat aagaaatatc cacgccgaag ataaaatttt ctccatcttg
     2281 atctaaccca ttaattattg agcataatgt aggcagtaca aaataagtta ggcgggatat
     2341 caggcgtcaa gaatggagag cgagaactct ccattcttga cacctgatat tgcggacata
     2401 ataagaaagc ataacgcctg aaatgctcac tttgcatcag catggtgata cagctgatgt
     2461 ttattctaaa accttactca agttctaaga gagcacggat tccctgtcat taataatgat
     2521 atccgttagt gtagtcttat gcatttcaat gtactgcaat atttgcagat agagatcaga
     2581 aagaatcaca ctggtttcat cttttaaacg caagcgtaaa gcctgatcaa taatatgact
     2641 atcaacatac ccatgcgtca tgaacacctc cctctcgaga tttttaaaca aagcacattg
     2701 aaaaataaat ctctctggga ttgaattatt catgtcagcc tcgtagaaat attaacccaa
     2761 taaaattatg ctgtgtatag tctaaagcga cagacaaaaa taagatgaat gagttttcta
     2821 taaacttata cttaataatt agaagttaca tatcatcagc tgtgtatcgc aacacgattt
     2881 ccagtgtttt tctaatcaca tcagctttga gaatatcact tg
//
LOCUS       DAZGJW010000315         2916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZGJW010000315 DAZGJW010000000
VERSION     DAZGJW010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.318"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..520)
                     /locus_tag="ACHKIK_005187"
                     /pseudo
     CDS             complement(1..520)
                     /locus_tag="ACHKIK_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc31 family transposase"
     gene            1082..2679
                     /locus_tag="ACHKIK_005188"
                     /pseudo
     CDS             1082..2679
                     /locus_tag="ACHKIK_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2709..2916)
                     /locus_tag="ACHKIK_005189"
                     /pseudo
     CDS             complement(2709..2916)
                     /locus_tag="ACHKIK_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          716 a          722 c          746 g          732 t
ORIGIN      
        1 gcccccgcag agcccctact ttggttccac gcccggcgta tttcgctgca caacctgact
       61 cgcgccggat taaccgtatc gcgtcgtttt cgccagtacc ggtaactgct gcggtgtatt
      121 tccagagcag aacagaggct gacaactgag tggctgtcac tcagtctggc aactatcgtg
      181 aaccgttcag cgagtcggac atcaagagcg cggtagcctt ttttaatatc gtattttgtt
      241 cctccaagcg gcgaacctgc ttttccagtt cgcggatacg ttgctggtct ggagtaatag
      301 atgtggcaga gggggcaatc ccctgacgct ctcgcctgag ctggcgcacc caactctcaa
      361 gcgtggtaga accgacattc atcgcttcac tggcttgtcg atatgagtag cccttatcaa
      421 caattagctg tgcacattcc agcctgaatt caggggtgaa agtacgtttg gttttcttgt
      481 tcattaagtc acctgttttg tgttgtggtg aggatatcac ctttaatcag gtggccaaat
      541 ttactgtgcc actacacttc aggggttttg aggtttttgg ccagttcgtt agccagagcc
      601 tgtaactgtt tttcgtccat aaattaacct tcatttgatg ctggattgaa catatcaaaa
      661 tcaggcaatt acacaaatct atgtacaggc tcgccgggca ctttacgctt tgacacaggg
      721 ggctaaatca tgagcgattc agatgccatt tttgcataca gagagttgac agcattccca
      781 tgctaattgt taaggtttaa attgaagtat atctatctgt catttttgca aatgacatgc
      841 ggtagcataa cgttccgggg gctcttatgc cgacatacgt ggcgacatta accactccca
      901 gatacagcca gccttcatcc gtacgtaatt gcgtgatgtc tcgcacccac ttctggttct
      961 ggccactgac gtaaaaatcc tgctcctgtc acgaacggtg caaaagtggt tcttccgcaa
     1021 cctgggtgga agaaaatgga tgaaactttc tggtgaataa ggatctaagc tgttttaagt
     1081 tatgggcaac gcaatgcact ctcttaagac acttctacaa ttaccttacg gatggcgatg
     1141 cagtcgacaa attattaact ctgacggtat aaccctccat ctccgcggaa aacgtaaaac
     1201 agcacaatgt cctgaatgct ttaagcgtag ctactctgtt catagttgtc gtcggcgtcg
     1261 gatacagcat ctcccctgtt cagggcagac gctatggctt gtattttccg tccgccacag
     1321 gtacagccgt aacccggctt gttcacgaaa aatttttgcc gggtcgctta ctcccttcgc
     1381 cggttcacac cagcagtctt cacaggcgtt acaaaattta caacgtcaac tgggattaat
     1441 tgccggaggt gaggctggaa aacgggcagc aacggtagtg ggtctccgtt gcagtgcaga
     1501 tacccttctt cgcagagtta tcaatacccc ggagacgaaa ctgtcaggct cgccccatgt
     1561 cggtattgat gagtgggcgt ggcatcgggg ccaccgttac ggtacgttaa tagtcaatct
     1621 tgatactcac cgtcctctcg tcctgcttcc cggccgcgat cagcgtacgc tggcgacctg
     1681 gttcagaaaa tatccggaaa tacaggttgt ctcgcgtgat cgcagtggaa tctatgcgac
     1741 agcagcacgt gaaggtgcac ctcaggccag acaggtggcc gatcgatggc acctgctaaa
     1801 aaatattggc gatgcgtttg aacgaatgat gtacagacat atgcctctga tacgtcttgt
     1861 tgccagtgag ttgtcaccaa agaaatcacc tgagccagaa cagtctgtgc ctgcagcatc
     1921 gctccgtcgt ccggaacgcc ttaaacagca aacctgcaaa aaacggcatc agcgttggac
     1981 agaggttatg gccctgcata acaagggatg tagtttcagg gaaatatccc gtattacagg
     2041 cctgtcgcga gtgacagtca gtcgctgggt gggttcagga acattccctg aaatgtcaac
     2101 caggcttcca aagcgaggct tctggcccca tggagggagt ggttaaaaga gcaacgagaa
     2161 agtggtaatt ataacgccag ccggatatgg cgggaaatgg tggcccgggg gtttacaggt
     2221 attgaaacca tcgtcaggga tgctgttgcc aaatggcgta atggctggat cccaccggtt
     2281 actactaccg ccagacttcc ttcagtgtcc cgggtaagcc gatggttgat gccctggaga
     2341 ataatcaggg gtgaagaaaa ttatgcttcc cgatttatta gtctggtgtg tgaaaaagaa
     2401 ccggagctga aaatagcgca gcaactggta ctcgagttct accgtattct gaaaacccaa
     2461 aataaatcac agccaagcag ctggttcact cgagtccacg aaagcggttc agcagaactt
     2521 cggcgcgtag ctgcggggat ggaagctgat gctgcggcta tatgtgaggc aatcagcagt
     2581 cgctggagta atggtgttgt cgaaggtcat gtaaatcgcc tgaagatgct gaaatgtcag
     2641 atgtatggtc gagccatgga acgtagttct caccaataaa taagcgtcaa tgcggtgctc
     2701 cattgacgct taccgttaaa atggtaaccc tgcgcacaca gttcatccgt caggcgtggt
     2761 acaccgtagc gctgttttga ccggataaaa gccgcgagga caacgctgcc gcagtgctga
     2821 cggaactgct gacatgtact tatccttgtc cgccgctgac accacatata ccagtcgctg
     2881 cggcccttcc ggagcacgtg gcacattgcc gtgatg
//
LOCUS       DAZGJW010000316         2915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZGJW010000316 DAZGJW010000000
VERSION     DAZGJW010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.319"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1346)
                     /locus_tag="ACHKIK_005190"
     CDS             complement(<1..1346)
                     /locus_tag="ACHKIK_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9086137.1"
                     /translation="MDIEIRHCNNIVRAHITLTADKLNIKFAPNGTGKSTLSRAISCA
                     ARDDIQGLQALMPFRLRGENPDSTGPIVIGADGIGDVMCFNEEYVSQFTFQPDELISD
                     SFNILIRNQAHAEREREIEEMTQKIRAVFTDHTELNSLIDHLQELSNAFKSTSSGISR
                     SSTGMRGLSGGNKIHHIPAGLENYQPYIRSERRVEWIDWQTKGLEFSPLSDGCCPFCT
                     GDITGKEAQIRQVREEYDKSTIKNLTAIIRLVENLGNYLTESARERLLAITMLQNGPE
                     AEHIEYLVALKRQTDTLTEKLTALRGLNVFSLQEQQNVREVLTARLIDLQFFPDLQSE
                     LMQGITDRLNAALMDLINLAGPLQGKINRHRDSMIRLIAQHKTNINNFLTYAGYKYRV
                     DIAGEGEQRKLRLRHIDFDGYVSGGSQHLSYGERNAFAIVLFMYECLSKNPGLIIL"
     gene            1664..1915
                     /locus_tag="ACHKIK_005191"
     CDS             1664..1915
                     /locus_tag="ACHKIK_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086138.1"
                     /translation="MDKERGFSLSYEQLTRIAEKNIRQCGLDSQIARCISELKASASL
                     WLWYELAIHGAPQNNHAQAQRIDTDYKRLKKLIWPEGDS"
     gene            1915..2505
                     /locus_tag="ACHKIK_005192"
     CDS             1915..2505
                     /locus_tag="ACHKIK_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086139.1"
                     /translation="MNQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLVRSGEA
                     PVYGPNLRASCRRLEDAGWLRILRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQVNESITP"
     gene            complement(2523..2870)
                     /locus_tag="ACHKIK_005193"
     CDS             complement(2523..2870)
                     /locus_tag="ACHKIK_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO9086140.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGTSWGCAMWFIYWGPSGMVAGEAIIISITSGFLSGLCMASFHWW
                     RRKVNRLPPWDDV"
BASE COUNT          680 a          755 c          774 g          706 t
ORIGIN      
        1 tcaaggataa tcagtcccgg gttttttgac aggcattcat acatgaataa cacaatcgca
       61 aaggcgttcc gttctccata gctgagatgc tggctaccgc cgctgacgta cccgtcaaaa
      121 tctatgtgtc gtagcctgag ttttctctgc tctccctcgc cggctatatc caccctgtat
      181 ttatagcctg cataagtgag gaaattattg atgtttgttt tgtgctgtgc gatcagccgg
      241 atcatgctgt cccggtgccg gttaattttt ccctgcagcg gtccggcaag gtttatcagg
      301 tccataaggg ccgcgttcag tctgtcggtg atcccctgca tgagttcgga ttgcagatca
      361 gggaagaact gcaggtcaat caacctggct gtgagcacct cccgaacgtt ctgctgttcc
      421 tgcaggctga aaacattcag gcctctcagc gcagtgagtt tctctgtcag cgtatcggtc
      481 tggcgtttca acgccactag gtactcgata tgttcggctt ccggaccgtt ctgaagcata
      541 gtgatggcca gaaggcgttc tctggcgctc tccgtcaggt aattaccgag gttttccacc
      601 agtctgatga tagcggtcag gtttttaatg gtggatttat cgtattcctc tctgacttgg
      661 cgaatttgtg cttcctttcc cgtgatatca ccggtacaga acggacagca gccatccgaa
      721 aggggcgaaa actcgagtcc ctttgtctgc cagtcaatcc attccacgcg gcgttcactg
      781 cggatgtagg gttgatagtt ctcaagacca gccggaatgt ggtgtatttt atttccgcct
      841 gacagacctc tcatgccggt tgaagaacgt gaaatcccgg aactggttga tttgaaagca
      901 ttactgagtt cctgtagatg gtctatcaga gagttcagct cagtgtgatc cgtgaaaaca
      961 gcccggatct tttgcgtcat ttcttctatt tcacgctctc tttccgcatg ggcttgattg
     1021 cggataagaa tattaaagct gtcactgatg agctcgtccg gttggaacgt aaactgactg
     1081 acgtactcct cgttgaagca catcacgtcc ccaatcccgt cagcaccgat gacaatgggc
     1141 ccggtgctat cggggttttc tccgcgcagc cggaagggca tcagtgcctg tagtccctga
     1201 atgtcgtccc gcgccgcaca gcttatggcc cgtgacagcg tactttttcc agtaccattt
     1261 ggcgcaaact taatgttcag tttatcggca gtgagggtga tatgtgctcg tacgatgtta
     1321 ttgcagtggc gtatttcaat gtccatttat ctgtcctttg ccagttattc cggggggatt
     1381 catatcctgc cgcaaaatgt gcccgcatgc tgtgatgcag atacataata catttcgttt
     1441 ataccattgc ttacacctct ccgaggtgtt catatcagaa acaaagatta ggtcgatccg
     1501 acttagccgg aaaccggatc tgagcgacgg ggaacgggaa agaaatataa taattaatca
     1561 atacacataa aatcatatct gatattatat gaaatcaata acacataaat catacaggat
     1621 atgattttta cctgtcagaa cattatcaga tggagcgtgc aggatggata aagaaagggg
     1681 gttttcactc agctatgagc aactgaccag aatagcggaa aaaaatatcc gtcaatgtgg
     1741 gctggacagc cagatcgcca ggtgcatcag tgaactgaag gcctcagctt cgctgtggtt
     1801 gtggtatgaa ctggccattc atggtgcgcc acagaacaat cacgcacagg cccaacgcat
     1861 cgataccgat tacaagcgcc tcaaaaaact catctggccg gagggtgaca gttaatgaat
     1921 cagagccgcc gaccgtcgcc actgcagcgg agagtgctga ttgtgctggc tgcccttgat
     1981 gaaaaacggc cgggacccgt gctgacgcgg gacatagaac gggtgctggt gcgaagcggg
     2041 gaagccccgg tctacgggcc aaacctgcgc gcctcctgcc gtcgtctgga ggatgccggc
     2101 tggctgcgta tcctgcgcgc tccgaacctg cagttggcag tggaactgac ggacgccggc
     2161 cgggccgtgg cacagcctct gctgctggca gaacaggacc gcctccgggc agaacagcgt
     2221 gcggcagaag tcgtcgtact gccgctggtc ccggcagcag gtctgcccgc agacggcact
     2281 tctgccactg atctggcggt ggagctgaac ggcataacgt atcaggcctg ccgtggggat
     2341 tttgtggtgc gtctcgacgg cagcacctgt ctgcagttat ggaataaaga aggcagggtg
     2401 gtacgtcggg agggcgatcc gctggaagtg gcgcagtggc tgcaggcctg tcacgatgca
     2461 ggaatggagg ttcgtgtgca ggttaatgaa agcatcaccc cataaaaaca tgcctgcacg
     2521 atttacacat catcccatgg tggcagccgg ttaactttcc tgcgccacca gtggaaagag
     2581 gccatgcaca gtccggacaa aaaaccactc gtgatactta taataatggc ctcacctgct
     2641 accattcctg acggccccca gtagataaac cacattgcac atccccagga agtgccccat
     2701 aagccccccg tcagcaccgt gacctgccag aacggcataa agggtaatgg cggaagccgg
     2761 atacccagcc gccagagaat acgcagaaga ggaggggcat aattgctccg ccacatcttt
     2821 ttgctgtcca tcagggcaat ggcccgggct tttttctgct caaaggtcac aggagcacct
     2881 ccgttccatg atgtaaacgt gacacagctt attgt
//
LOCUS       DAZGJW010000317         2899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZGJW010000317 DAZGJW010000000
VERSION     DAZGJW010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.320"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..937
                     /locus_tag="ACHKIK_005194"
     CDS             <1..937
                     /locus_tag="ACHKIK_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO9086141.1"
                     /translation="FNWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDF
                     LNGDPDQPIIMGRTYHHENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQV
                     YIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQII
                     NVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKV
                     GNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKE
                     QVNGDSLLINWNCAASKSPPKPPDEKQDTPDMREY"
     gene            1005..>2899
                     /locus_tag="ACHKIK_005195"
     CDS             1005..>2899
                     /locus_tag="ACHKIK_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO9086142.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVQPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDI
                     RLQIRDDALILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWA
                     SLPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDLLPAPLPPYRVLTGMADRFGR
                     TLAYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKPHTASLSSPDSPRPL
                     SAPSFPDTLPGTEYGADSGIRLSAVWLMHDPEYPENLPAAPLVCYDWTPRGELAAVYD
                     RSGTQMRHFTYDDKYRGRMVGHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQD
                     RITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTE
                     YGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSR
                     SGETVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQ
                     MTAVHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQY
                     DAWGKAV"
BASE COUNT          656 a          788 c          950 g          505 t
ORIGIN      
        1 gttcaactgg gaccgttata acccggcaga ccaagacagt tcgtgctgga tccgtgtggc
       61 acaggcgtgg gcaggcaccg gttttggcca cctggcgata ccgcgtgtgg gtcaggaggt
      121 gattgtggac ttcctcaacg gcgacccgga ccagccgatt attatggggc gtacctacca
      181 ccatgaaaac cgcacacccg gcagcctgcc ggggacgaag acgcagatga cgatccgctc
      241 gaaaacctat aagggcagcg ggtttaatga actgaagttt gacgatgcga cagggaaaga
      301 acaggtctac atccacgcgc agaagaacat gaacaccgag gtgctgaata accgcaccac
      361 ggatgtgata aacaaccatg ctgagaccat tggcaacaat cagatgattg cggttaccaa
      421 caatcagata cagacggtgg gcgttaacca gatagagacg gtgggcagta accagatcat
      481 taacgtgggt tctgttcagg tggaaacgat tggacttgtt cgtgcgctga ccgtgggcgt
      541 ggcgtaccag acgacggtag gtggcattat gaacacttcg gtggctctga tgcagtcctc
      601 gcagatcggt ttgcataaat cgttgagggt cgggctgggt tatgacgtca aagtcggaaa
      661 taacgttacc ttcaccgttg gtaaaacgaa aaaggatgat accgggcaga ccgcgattta
      721 ctccgccggt gagcatctgg agctctgctg tggtaaggca aggctggtgc tgacgaagga
      781 cggacaaatt tttctcaacg gcacaaaaat tcatttgcag ggtaaggagc aggttaatgg
      841 tgactcactg ttgattaact ggaactgtgc agcctcgaaa tccccaccga agccccctga
      901 tgaaaagcag gatacgccgg atatgagaga gtactgatcc cgtacctgtt cggaattcag
      961 taacaaacca gcattaaaaa taaacagaag aaggaatttg aatcatgagc ggaaaaccag
     1021 cggcgcgtca gggagatatg actcagtatg gcggtcccat tgtccagggt tcggcaggtg
     1081 taagaattgg cgcgccaact ggcgtggcgt gctcggtgtg tccgggcggg atgacctcgg
     1141 gcaacccggt gaacccgctg ctcggtgcaa aagtacagcc cggcgagacg gaccttgcgc
     1201 tgcccggccc gctgccgttc attctctccc gcacctacag cagctaccgg acgaagacgc
     1261 ctgcaccggt gggcgttttc ggccccggct ggaaagcgcc ttctgatatc cgcttacaga
     1321 tacgcgatga tgcactgata ctcaacgaca acggcgggcg gagcattcac tttgagccgc
     1381 tgctgccggg ggaggcggtg tacagccgca gtgagtcaat gtggctggtg cgtggcggta
     1441 aggcagcgca gccggacggc cacacgctgg cacggctgtg ggcgtcgctg ccaccggata
     1501 tccggttaag cccgcatctt tacctggcga ccaacagcgc acaggggccg tggtggatac
     1561 tggggtggtc tgagcgggtg ccgggtgctg aggacttact gccagcgccg ctgccgccgt
     1621 accgggtgct taccgggatg gcggaccgct tcgggcggac gctggcgtac cggcgtgagg
     1681 ccgccggtga cctggccggg gaaatcaccg gcgtgacgga cggtgccggg cgggagttcc
     1741 gtctggtact gaccacacag gcacagcggg cggaagaggc ccgtaaaccg cacaccgctt
     1801 cgttatcttc ccctgacagt ccccgccctc tatcagctcc ttccttcccc gacacactgc
     1861 ccggtacgga atacggcgct gacagcggta tccgtctttc ggcggtgtgg ctgatgcacg
     1921 acccggaata cccggaaaat ttacctgccg cgccactggt gtgctacgac tggacgccac
     1981 gcggtgagct ggcggcggtg tatgaccgca gcggtacgca gatgcgccac tttacttacg
     2041 atgataaata ccggggccgg atggtggggc accgttatgc aggcaggccg gagatgcgct
     2101 accgctacga cgatgcgggg cgggtggtgg agcaacttaa cccggcaggc ctgagttacc
     2161 gctaccagta tgagcaggac cgcatcaccg tcacggacag cctgaaccgg cgtgaggtgc
     2221 tgcatacaga aggcggggcc gggctgaagc gggtggtgaa aaaagaactg gcggacggca
     2281 gcgtcacgca cagcgggtat gacgcggcag gaaggctcac ggcgcagacg gacgcggcgg
     2341 gacggcggac agagtacggt ctgaatgtgg tgtccggcga tatcacggac atcaccacgc
     2401 cggacgggcg ggagacgaaa ttttactata acgacgggaa ccagctgacg gcggtggtgt
     2461 ccccggacgg gctggagagc cgccgggaat atgatgaacc gggcaggctg gtatcggaga
     2521 catcgcgcag cggggagaca gtacgctacc gctacgatga cgcgcacagt gagttaccgg
     2581 cgacgacaac ggatgcgacg ggcagcaccc ggcagatgac gtggagccgc tacgggcagt
     2641 tgctggcgtt cactgactgc tcgggctacc agacccgcta tgaatacgac cgcttcggcc
     2701 agatgacggc ggttcaccgc gaggaaggta tcagccttta ccgccactat gacaaccgtg
     2761 gccggttaac ctcggtgaaa gacgcacagg gccgtgaaac gcagtatgag tacaacgccg
     2821 caggcgacct gactgccgtt atcaccccgg acggcaaccg gagcgagaca cagtacgatg
     2881 cgtggggaaa ggcggtcag
//
LOCUS       DAZGJW010000318         2898 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZGJW010000318 DAZGJW010000000
VERSION     DAZGJW010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.321"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1792
                     /locus_tag="ACHKIK_005196"
     CDS             <1..1792
                     /locus_tag="ACHKIK_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086143.1"
                     /translation="GELREFELADGNWLLNNAWYERNMAGFNEQLKENLSFTPDELKT
                     LFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICASCGLTEVHHA
                     PENHKPDPEWYCTSLCRETETLCQEIYERPYNSFISDATANGLILMKLPETWSTNEKM
                     FASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEIQTKYCSTAAR
                     SVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVPGVTDPAEASR
                     LIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTASVFWLSTGDRD
                     AALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPTVRLALQKGTG
                     AGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGTQFIRNLAVASSGVAGGA
                     VGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEEDRVKILAMIQE
                     QVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNMLIKPLVVSII
                     RQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            1868..2323
                     /locus_tag="ACHKIK_005197"
     CDS             1868..2323
                     /locus_tag="ACHKIK_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO9086144.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            2402..2635
                     /locus_tag="ACHKIK_005198"
     CDS             2402..2635
                     /locus_tag="ACHKIK_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO9086145.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            2735..>2898
                     /locus_tag="ACHKIK_005199"
     CDS             2735..>2898
                     /locus_tag="ACHKIK_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HFO9086146.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLE"
BASE COUNT          742 a          725 c          760 g          671 t
ORIGIN      
        1 gggagagctg agggaatttg aactggcaga cggaaactgg ttactgaaca atgcctggta
       61 tgaaagaaac atggcagggt ttaacgaaca gttgaaagag aacctgtcat tcacacctga
      121 tgaactgaaa acgctcttcc ggaaccgcct gaatttatca ccggaagcga atgacgattt
      181 tctcgatcgt tgcctggacg gcggtgactg gtatcccttt tcagaaggtc gccgttttgt
      241 atcattccat cacgtggatg agcttcgtat ctgtgcctcc tgcgggctga cagaagtaca
      301 tcatgccccg gaaaatcata agccggatcc ggaatggtac tgcacctctc tttgtcgcga
      361 aacagaaaca ctgtgtcagg aaatttatga acgcccttac aacagcttta tttccgatgc
      421 aacggcgaat ggtctgattc tcatgaaact gccggaaacc tggagtacaa atgagaaaat
      481 gtttgcttcc ggagggcagg gacatgggtt tgccgctgaa cggggaaacc atattgtcga
      541 cagagtccgt ctgaaaaacg cacggatcct cggtgataat aatgccagaa atggagcaga
      601 cagactggtc agcggaacag aaatccagac gaaatattgt tcaactgcag cccgtagcgt
      661 cggtgcggca ttcgacggac aaaacggaca gtatcgttac atgggaaata atggccccat
      721 gcaactggaa gtcccccgtg atcagtatgc cggcgctgtt gaaaccatga ggaataagat
      781 ccgcgaaggt aaagtacccg gtgtaaccga tcccgcagaa gcatcccggc tgattcgtcg
      841 gggacatctg acttatactc aggcccgtaa tatcacccgg ttcgggacca tcgaatcggt
      901 cacttatgat attgccgagg gttcggttgt cagtctggcg gccggaggga tcagttttgc
      961 cctgacggca tcggtcttct ggctcagcac cggcgatcgc gatgctgccc tgcagacagc
     1021 tgctgtccag gcaggaaaaa ccttcacccg cacactggct gtctacgtca caacccagca
     1081 actccaccgg ctcagtgttg ttcagggtat gctgaagcac attgattttt cgactgccag
     1141 cccgactgtt cgactggctc ttcagaaggg gaccggtgca ggaaatatca gtgccctgaa
     1201 caaagtgatg aagggtacgc tggtgacatc tctggcactg gtagctgtca caaccggccc
     1261 tgacatgatc aaaatgttgc ggggacggat ctccggtacg cagttcatca ggaatcttgc
     1321 cgtggcatct tccggtgtgg caggtggtgc tgtcgggtca gtggcgggcg gaatactgtt
     1381 cagtccactg ggaccatttg gtgcactgac agggcgtgtg gttggcggtg ttctgggggg
     1441 aatgattgcc tccgctgtat caggaaaaat tgccggagcg ctggttgaag aagatcgcgt
     1501 caaaattctg gcaatgattc aggagcaggt gacatggctt gccggcagtt tcctgctgac
     1561 cggacatgag attgaaaatc tgaacgagaa tctggcccgt gttatcgatc agaatgctct
     1621 ggagatcatt ttcgccgccg gtatacaaca acgggccgcg accaatatgt taatcaaacc
     1681 actggtggtc agtatcatca ggcaacgccc cgtcatggaa tatgatgcat cccatctcgg
     1741 caatatggtt aaccgactgg aagaagcatt acccccggag ttaccggcat aacggctatt
     1801 ctgcgccttt tacgctttat cacctgattt gtcagtgtca tcgtcatgac tcagagaggt
     1861 aaataccatg attcacctgt tcaaaacctg catgattacc gccttcattc tggggttaac
     1921 gtggtctgcc ccactccggg cacaggatca acgttacatc agtatacgca atacagatac
     1981 gatatggctc ccgggaaata tttgtgctta ccagttccgg ctggataatg gcggaaacga
     2041 tgaaggattt ggccccctca ccatcactct gcaactcaaa gacaaatatg gtcagacgct
     2101 ggtgaccaga aaaatggaaa cggaagcttt tggtgacagt aatgccacgc gaaccacaga
     2161 cgcatttctg gaaacggagt gcgtggaaaa tgtcgccaca accgaaatca tcaaagcaac
     2221 tgaagaaagt aacggccatc gtgtcagtct gccgttatcg gttttcaatc cccaggacta
     2281 ccatccactg ctgattaccg tttccggaaa aaacgttaac tgatccctga agtcagtttc
     2341 atgcttcagc ctctgtcaca acgcgtggca gaggcttttt atttatcagg agaaaacaga
     2401 tatgccgggt tacacagagt atgtcctggc tgaaggatct ttctcatacg ggcaggcggt
     2461 tgctgtcata accgcttacc gcaatgtgtt tatccaggat gacccgggaa tgcatttccg
     2521 tcgggttatc cgcaacgcgg aaggtcagcg acgctggcgc tgccggaatt ctgaacccga
     2581 tgccggaaag gtgcttaata cgcggctcgc cagtgacggt cttctcagac aataaacgtc
     2641 attcatttca cccatcaggc cgcgtcttct ccgggagacg tggctttttc atttattaca
     2701 ccagtaaatc ttaaccaccg ataaggagca aattatgcga ctagccagtc gttttggtta
     2761 tgcgaaccag atacgccgtg accgtccgct gacacacgaa gaactgatgc actatgtgcc
     2821 ggggattttc ggggaagata aacacacgtc ccgaagccag aactatacgt acatccccac
     2881 catcaccgta ctggaaag
//
LOCUS       DAZGJW010000319         2895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZGJW010000319 DAZGJW010000000
VERSION     DAZGJW010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.322"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            154..1365
                     /gene="bluF"
                     /locus_tag="ACHKIK_005200"
     CDS             154..1365
                     /gene="bluF"
                     /locus_tag="ACHKIK_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO9086147.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            1570..2301
                     /gene="bluR"
                     /locus_tag="ACHKIK_005201"
     CDS             1570..2301
                     /gene="bluR"
                     /locus_tag="ACHKIK_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO9086148.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2522..>2895
                     /locus_tag="ACHKIK_005202"
     CDS             2522..>2895
                     /locus_tag="ACHKIK_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO9086149.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYI"
BASE COUNT          840 a          618 c          624 g          813 t
ORIGIN      
        1 aatctataca agatttttcg ccaactagtg ttcaactttt tccatttttt atcaatgtaa
       61 agaaacggtt atgttttggt attaaaagaa agacacaaga agatatgtct gttaccgtct
      121 tactctcgcc tcacccatta ccctggattg ttaatgctta ccacccttat ttatcgtagc
      181 catatacgtg acgacgaacc tgtcaaaaaa atcgaagaaa tggtttcgat agcaaatcgc
      241 aggaacatgc agtctgacgt aacagggatc ttactgttta atggttctca ttttttccag
      301 cttctggaag gtccggaaga acaggttaaa atgatatatc gggctatatg ccaggatcca
      361 cggcactata atattgttga gctgctgtgc gattacgcgc ctgctcgccg ttttggcaaa
      421 gcgggaatgg aattatttga tttgcgcctg cacgagcgag atgacgtttt acaggccgta
      481 ttcgacaaag gcacatcaaa atttcagcta acttatgatg acagagcgct acaatttttt
      541 cgtacttttg tccttgcaac cgaacaatca acctatttcg agatccctgc cgaagactcc
      601 tggcttttta tcgctgacgg atctgataaa gaacttgatt cctgtgccct ttcaccaact
      661 ataaacgacc actttgcctt tcatcctatt gtcgatccct tatcgcggcg gataatcgct
      721 tttgaagcca ttgtgcaaaa aaatgaagat agcccatcag ccatagcggt tgggcagcgt
      781 aaagacgggg aaatctacac agcggatctc aaaagtaagg cgcttgcatt cacgatggca
      841 cacgcacttg agctcggtga taaaatgatt tcaatcaatc tattacctat gaccctggtt
      901 aacgaacctg acgcagtctc ttttttactt aatgaaataa aggccaatgc tctggtgcct
      961 gaacaaatca tcgttgaatt tactgaaagt gaagtcatat ctcggtttga tgagtttgcc
     1021 gaagcgatta aatcgctaaa ggctgctggt atcagtgtag caattgatca ttttggcgca
     1081 ggttttgctg gtttgttact cctgtcacgc ttccagcctg acagaattaa aatcagtcag
     1141 gaattgatta ccaatgttca taaaagtggg ccacggcagg caattattca ggcgatcata
     1201 aaatgctgta catcacttga aattcaagtc agtgctatgg gcgtggcaac accagaagag
     1261 tggatgtggc ttgaatccgc aggaattgag atgtttcagg gagatctgtt tgctaaagct
     1321 aaattgaatg gtatcccttc catagcgtgg ccagagaaaa aataattttc agcacattct
     1381 ttcacatgat ttcagtaaat catcgtgaaa atgaatacgt tacgtttaaa tttaatccac
     1441 attaaaaata atgtttcatc caatttagct gaaatcttat ttatactttg tacaatttta
     1501 gcgtatattg tacaaagtat tattgggtcg tgtacaggcg acggagattt gtgaccgcaa
     1561 ggaggaattg tggcttatta cagcattggt gatgttgctg aacgttgcgg gattaatcct
     1621 gtcactctcc gggcctggca acgccgctac ggtttgttaa aaccacagcg cagtgaaggc
     1681 ggacaccgac tctttgatga agaagacata caacgcatcg aagagatcaa gcgttggata
     1741 agtaatggcg tccctgtagg caaagttaaa gcattactgg aaaccaccag ccaggatacg
     1801 gaagatgact ggagccgcct gcaagaagag atgatgtcaa ttcttcgcat ggctaatcct
     1861 gccaaactac gcgcgagaat tatttcactg ggtcgagagt acccagtaga tcaattgatt
     1921 aatcatgttt accttcctgt tcgccagcgt ctcgtgcttg atcacaacac ctcccgcatt
     1981 atgagcagta tgtttgacgg cgcattaatt gaatacgcag caacctcgct tttcgaaatg
     2041 cgccgtaagc ccggtaaaga agccattctg atggcgtgga atgttgaaga gagagcacga
     2101 ttgtggctgg aagcatggcg tttatcattg tcaggatggc acatttctgt tcttgctgat
     2161 cccattgaat cgccgcgccc ggaactgttc ccgacgcaaa cattgattgt ctggacaggc
     2221 atggcaccaa cgagaaggca aaacgaactt ttacaacatt ggggtgagca aggttataaa
     2281 gtcatctttc atgcccccta atttcacctt tatcccgccc cttagacaga atgttataat
     2341 ctgaacccac aacagcgttt gtaccggagc tggtccgttg caaaatgctc accgggagaa
     2401 ttcagatatt ccctctatca cactctgtac atatcacagc caaaggacag accagattga
     2461 agtggtaaaa ccgatcgtgc acactacgca gtctcacaac taacaagagg ttattatcct
     2521 gatggatcag gtcgttgttt ttcaaaagat gtttgagcaa gtgagaaaag agcaaaactt
     2581 ctcctggttt tattcagaat taaaacatca ccgtattgca cattacattt attatctggc
     2641 tacggataac atcagaatta ttactcacga tgacacggtt ttgttattaa gaggaaccag
     2701 gaacctgtta aaagttagta cgaccaagaa ccctgctaaa ataaaagagg ccgcattgct
     2761 tcatatttgc ggaaaatcta catttcggga atactgttca acactagcag gcgcgggcgt
     2821 tttccggtgg gttactgatg ttaatcataa caaacgcagt tactacgcca ttgataatac
     2881 gcttttatat attga
//
LOCUS       DAZGJW010000320         2878 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZGJW010000320 DAZGJW010000000
VERSION     DAZGJW010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.323"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..2011
                     /locus_tag="ACHKIK_005203"
                     /pseudo
     CDS             2..2011
                     /locus_tag="ACHKIK_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
     gene            2080..2703
                     /locus_tag="ACHKIK_005204"
     CDS             2080..2703
                     /locus_tag="ACHKIK_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO9086150.1"
                     /translation="MRHLYATILLFTTLLAGIAFPAQAESGHGAFSVGYAQVHPGGVP
                     ALSGTGARAGDLKGINVKYRYEFTDHLGGIVALSYASVKKSDTMKTGENTFHYESLRG
                     RYVSLMAGPVWQLSERVSLYGMAGMAYTRWSDSVQDYRRDEVKPGYVKETTTASDGHT
                     ARHLSPAWNAGIQFSPVETVVIDLAYEGSASGDWRTDGFIVGVGYKF"
     gene            2768..>2878
                     /locus_tag="ACHKIK_005205"
     CDS             2768..>2878
                     /locus_tag="ACHKIK_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO9086151.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNT"
BASE COUNT          677 a          729 c          872 g          600 t
ORIGIN      
        1 catgaccggc ggacgtgtcc tgtccatcga tgccgccagc cgcaccctga cactggaccg
       61 tgaggtgacc ctgccggaga caggtgccgc cacggtgaac ctgattaacg gcagcggtaa
      121 gccggtgagc gtggccatca ctgcacaccc cgcgccggac cggatacagg tcagcaccct
      181 gcctgatggt gtggagacat acggtgtatg ggggctctcc ctgccgtcac tgcgtcgtcg
      241 cctgttccgc tgtgtttcca tccgggaaaa cacggacggt acctttgcca tcacggcagt
      301 gcagcatgtg ccggagaaag aagccatcgt ggataacggg gccagctttg agccgcagtc
      361 aggcaccctg aacagcgtca tccctccggc agtgcagcac ctgacggtgg aggtgagcgc
      421 agctgacggc cagtatctgg cgcaggcgaa atgggacacg ccgcgggtgg tgaagggcgt
      481 gcgcttcagt ctgcgcctga ccagtggaag cggtcaggac agccgtctgg tgaccaccgc
      541 catcaccgcg gatacagagc accgtttcag cggtctgccg ccgggggaat acaccctgac
      601 ggtcagggcg attaacagtt atggccagca gggggaaccg gccaccacca cgttcaggat
      661 taatgcacct gcggtacccg ccacgattga gctgacaccg ggctattttc agataacagc
      721 ggtcccgcgt cttgcggtgt atgacccgac ggtacagttt gagttctggt tttcggagac
      781 aaaaatcgca gatacatctc aggtggaaac ctctgcccgt tatctgggga ccggcagtca
      841 gtggagtgta tccggcccgc acattaagcc cgggaaggat ttctggtttt acgtgcgcag
      901 cgtcaacctg gtggggaaat ctgcttttgt ggaagccagt ggccgggcca gcaatgatgc
      961 agaagggtat ctggggctgt ttcgggaaaa aataggaaaa ctgcatctgg ctcaggggct
     1021 gtgggagctg atagacaaca gccagcttgc ggatgagatg gcggagatga agaccaccat
     1081 caccgaaacc cgcaatgaaa tcacacagac ggtcagtaaa acgctggagg accagagcgc
     1141 caccatacag cagatacagc gcgtgcagaa ggacacaaat gatgaccttg ctgcacttta
     1201 catgctgaag gtacagaaaa caaaaaatgg cataccctat gttgccggta ttggagcggg
     1261 gattgaggat actgatggcc agcccctgag caacatactg ctgctggctg accgtattgc
     1321 gatgattaac ccggaggacg gcaacaccac gccgttattt gtggcgcagg ggaatcagtt
     1381 gttcatgaac gatgtgttcc tgaagcggct gtttgcggtg agtatcacct cgtccggcaa
     1441 tcccccgacg ttttccctga cgccggaggg caggctgacc gcaagaaatg ctgatatcag
     1501 cggagcaatt accgcgaata ccggcacgct caataatgtc accattaacg agaactgtgt
     1561 catcagaggg aaactgtctg caaaccagat tgaaggcgac ctggtgaaga cggtggggaa
     1621 agcctttccc cggaataaca gttatgccag cgggacggta accgtcacag tttacgatga
     1681 ccagggattc gaccggcaga ttatcattcc cccggtgctg tttcgcggga cgaaacacca
     1741 gaatttcaac agcccgaatc agcagtcgta ctggtattcc acctgtaagc tgcaggtgct
     1801 gaagaacggg gttgagattt tccatgaacc ggcaacggat gtcagccggg tgttctcatc
     1861 ggtgatagat atgccggcag ggcggggtca tgtcaccctg acgtttaatg tgtcgtcggc
     1921 cggtgcgaac aactggacgc cgacaacgta catcagtgat ttactggttg tggtcatgaa
     1981 aaaatccacg gcagggatca gtatcagctg acggtttatt aacccggacg ggcacctcag
     2041 gaggtgcctt ttttattgac tgaaaacaaa gaggtaatca tgcggcattt atacgcaacg
     2101 atattattgt ttactaccct gctggcagga attgcctttc ctgcacaggc tgaaagcgga
     2161 cacggtgcat tttccgtggg atatgctcag gttcacccgg gcggcgtacc ggcattgtcc
     2221 ggtaccggtg cgcgtgcagg tgatttaaaa gggattaatg tgaaataccg ttatgagttc
     2281 acggatcacc tgggcggcat tgtcgcgctg agttatgcat cggtgaagaa aagtgacacg
     2341 atgaagacgg gtgaaaatac cttccattat gaaagcctgc gcggtcgtta tgtcagtctg
     2401 atggccggcc ctgtctggca gctcagtgag cgggtcagtc tctatggcat ggccgggatg
     2461 gcgtacaccc gctggtctga cagtgttcag gattaccggc gtgatgaagt gaaaccgggg
     2521 tatgtgaagg agaccaccac cgccagtgat ggccatactg cgcgtcatct gtcgccggcc
     2581 tggaatgccg ggattcagtt cagtcccgta gagacggtgg ttattgacct tgcttatgaa
     2641 ggttccgcca gtggcgactg gcgcactgac ggtttcatcg tgggtgtcgg ttataagttc
     2701 tgattagcca ggtaacacag tgttatgaca gcccgccggt tcaggcgggc ttttttgtgg
     2761 ggtgaatatg gcagtaaaga tttcaggtgt actgaaagac ggcacaggaa aaccggtaca
     2821 gaactgcaca atccagctga aagcaaaacg taacagtacc acggtggtgg tgaacacg
//
LOCUS       DAZGJW010000321         2876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZGJW010000321 DAZGJW010000000
VERSION     DAZGJW010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.324"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1427
                     /gene="ehxB"
                     /locus_tag="ACHKIK_005206"
                     /gene_synonym="hlyB"
     CDS             <1..1427
                     /gene="ehxB"
                     /locus_tag="ACHKIK_005206"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFO9086152.1"
                     /translation="RHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            1431..2870
                     /gene="ehxD"
                     /locus_tag="ACHKIK_005207"
     CDS             1431..2870
                     /gene="ehxD"
                     /locus_tag="ACHKIK_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFO9086153.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINESLRER"
BASE COUNT          932 a          450 c          628 g          866 t
ORIGIN      
        1 tcagacattt gcttgcattg cctgtttcat attttgaaaa tcgcagggtc ggagagaccg
       61 ttgccagagt aagggaactg gagcaaattc gtaatttttt aaccggacaa gcgttgacat
      121 cagttcttga tctatttttt tctgtaatat ttttttgtgt catgtggtat tacagccctc
      181 aattaacact ggttatatta ttgtcactac cttgttatgt tatatggtca ttgtttatat
      241 cacccttatt acgtcgacgt cttgatgata agtttctcag gaatgcagaa aatcaagctt
      301 ttcttgtcga aacggtaaca gcaataaata caattaaatc catggcagta tcaccacaga
      361 tgattgctac atgggataaa caactggccg ggtatgttgc ttccagtttc agggtaaatc
      421 tggttgcaat gacagggcag caggggatac agctgataca gaaaagtgta atggtaatta
      481 gtctatggat gggagcacat cttgtcatat cgggagagat aagtatcggg cagttaatcg
      541 cgtttaatat gcttgcaggt caggtcattg ctcctgttat cagactggct catctttggc
      601 aggattttca gcaggttggt atttctgttg agcgccttgg tgatgtatta aatacaccgg
      661 tagagaaaaa gtcaggcaga aatatactgc cggaaattca gggggatatc gaatttaaaa
      721 atgtcagatt tcggtattct tctgacggta atgttatttt gaataatatt aatttataca
      781 tatcaaaggg ggatgttatc ggtatagttg gccgttctgg ttcaggaaaa agtacattaa
      841 caaaactgct tcagcgcttt tatataccag agaccggaca gattttaatt gatgggcatg
      901 atttatcact tgcagatcca gaatggttac gacgccagat tggtgttgta ttgcaggaaa
      961 atatactatt aaatcgtagt attatcgata atattacatt agcttctccg gctgtatcta
     1021 tggaacaggc tattgaggca gccagacttg caggtgccca tgattttatt acagaactaa
     1081 aagaagggta caatactatt gttggagaac agggcgttgg tctttcagga gggcagcgcc
     1141 aacggatcgc aatagcccgg gctcttgtta caaatcctcg aattcttatt tttgatgaag
     1201 caaccagtgc tcttgattat gagtcggaaa atataataat gaaaaatatg tcaagaatat
     1261 gtaagaacag aaccgtaatt attattgcac acaggttgtc aactgtaaaa aatgcaaaca
     1321 gaataattgt tatggataat ggctttattt ctgaagatgg cacacataaa gagcttatct
     1381 ccaaaaaaga cagtttatat gcatatttat atcagttgca ggcataacgt atgcgctttt
     1441 acatgaaagg tttatgggat ttggtttgcc gttataagac ggttttttct gatgtctgga
     1501 aaattcgtca cacacttgat gctcctgtga gagaaaaaga tgagtatgca tttcttcctg
     1561 ctcaccttga actcattgaa acaccagtat ccagacgttc tcattttgtt gtatggagta
     1621 ttttattatt tgtaattata tctcttctct tatctgttct tgggaaagtt gaagtggttt
     1681 cagtagcaaa tggtaagttt actcatagtg gaagaagcaa agaaataaaa ccgatagaga
     1741 atgcgattgt agaaaaaata atggtaaaag atggctcttt tgtaaaaaaa aatgatccat
     1801 tagttgaatt gacggtgcct ggtgttgaat ctgatatttt aaaatcagaa gcatctttgt
     1861 tgtatgaaaa aacagaacaa tatcgctacg caattctttc tgaatcaata caacggaatg
     1921 agcttcctga aataaggata acagattttc ctggcggaga agataacgcg ggaggtgaac
     1981 attttcagag ggttagctca ttaataaaag agcaattcat gacatggcag aacagaaaaa
     2041 atcaaaagca gctcacatta aataaaaaaa tagttgaacg ggatgcagcg cttgctcgtg
     2101 ttagcctgta tgagcatcag gtatcacagg aaggaagaaa actcaatgat tttaagtatt
     2161 tgttgaataa aaaagctgtt tctcaacatt cagttatgga gcaggaaaat agctatattc
     2221 aggcaaaaaa tgaacatgca gtctggcttg cacaggtttc tcaacttgaa aaagaaatag
     2281 aacttgtgcg ggaagaactg gcactggaga cgaatatctt cagaagtgaa attatcgaga
     2341 agcacagaaa atcaacagat aacattgtgt tgctggagca tgagcttgaa aaaaacaggc
     2401 agagaaaagc atcatctttt attaaagctc ctgtgagtgg tactgttcag gagttaaata
     2461 tacatacaga aggtggtgtt gtcacaacag cagaaacgct gatgattatt gtccctgata
     2521 atgatattct cgaagtaaca gcatctgtac ttaacaagga tatcggtttt atacaacctg
     2581 gacaggaggt cgttattaaa gtagatgcat atccatatac acgtcatggt taccttacag
     2641 ggaaagtaaa aaatataact gcagattctg tttctgttcc ggatacaggg cttgtattta
     2701 acgtgattat atctgttgat cggaatgata tacagggaga aagaaaaaaa atccctgtta
     2761 cagctggaat gactgttatg gcagaaataa aaaccggggt gcgtagtgtt atcagctatc
     2821 ttcttagtcc attaaaggaa actattaatg aaagtttacg tgaacgttag tatata
//
LOCUS       DAZGJW010000322         2813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZGJW010000322 DAZGJW010000000
VERSION     DAZGJW010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.325"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            273..1439
                     /gene="nhaA"
                     /locus_tag="ACHKIK_005208"
     CDS             273..1439
                     /gene="nhaA"
                     /locus_tag="ACHKIK_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO9086154.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1505..2404
                     /gene="nhaR"
                     /locus_tag="ACHKIK_005209"
     CDS             1505..2404
                     /gene="nhaR"
                     /locus_tag="ACHKIK_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO9086155.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2443..>2813)
                     /locus_tag="ACHKIK_005210"
     CDS             complement(2443..>2813)
                     /locus_tag="ACHKIK_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO9086156.1"
                     /translation="INNENYQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGT
                     SILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTY
                     GNNVSSGQIMSRVKIVVMYN"
BASE COUNT          666 a          625 c          702 g          820 t
ORIGIN      
        1 tgtcctaaca aataacgccg actgacaaga aattaattat tacttttcct aattaatccc
       61 tcagaaatcc tcaccttaag ctatgattat ctgggcttag ggtcactcgt gagcgcttac
      121 agccgtcaaa aactcatctc accgctgatg gcgcaaattc ttcaatggat cgtaaaaaac
      181 gagttattcc tacactataa tctgatttta acgatgattt gtgcggggta aaatagtaaa
      241 aacgatctat tcacctgaaa gagaaataaa aagtgaaaca tctgcatcga ttctttagca
      301 gtgatgcctc gggaggcatt attcttatca ttgccgctat cctggcgatg attatggcca
      361 atagcggcgc aaccagtgga tggtatcacg actttctgga gacgccggtt cagctccggg
      421 ttggttcact cgaaatcaac aaaaacatgc tgttatggat aaatgacgcg ctgatggcgg
      481 tatttttcct gttagtcggt ctggaagtta aacgtgaact gatgcaagga tcgctagcca
      541 gcttacgcca ggccgcattt ccagttatcg ccgctattgg tgggatgatt gttccggcat
      601 tactctatct ggcctttaac tatgccgatc cgattacccg cgaagggtgg gcgatcccgg
      661 cggctactga cattgccttt gcacttggtg tgctggcgct gttgggaagt cgtgttccgt
      721 tagcgctgaa gatctttttg atggctctgg ctattatcga cgatcttggg gccatcatta
      781 tcatcgcatt gttctacact aatgacttat cgatggcctc tcttggcgtc gcggctgtag
      841 caattgcggt actcgcggta ttgaatctgt gtggtgtacg ccgcacgggc gtctatattc
      901 tggttggcgt agtgttgtgg actgcggtgt tgaaatcggg ggttcacgca accctggcgg
      961 gggtaattgt cggcttcttt attcctttga aagagaagca tgggcgttct ccagcgaagc
     1021 gactggagca tgtgttacac ccgtgggtgg cgtatctgat tttgccgctg tttgcatttg
     1081 ctaatgctgg cgtttcactg caaggtgtca cgctggatgg cttgacctcc attctgccat
     1141 tggggatcat cgctggcttg ctgattggca aaccgctggg gattagtctg ttctgctggt
     1201 tggcgctgcg tttgaaactg gcgcatctgc ctgagggaac gacttatcag caaattatgg
     1261 cggtggggat cctgtgcggt atcggtttta ctatgtctat ctttattgcc agcctggcct
     1321 ttggtagcgt agatccagaa ctgattaact gggcgaaact cggtatcctg gtcggttcta
     1381 tctcttcggc ggtaattgga tacagctggt tacgcgttcg tttgcgtcca tcagtttgac
     1441 aggacggttt accggggagc cataaacggc tcccttttca ttgttatcag ggagagaaat
     1501 gagcatgtct catatcaatt acaaccactt gtattacttc tggcatgtct ataaagaagg
     1561 ttccgtggtt ggcgcagcgg aggcgcttta tttaactcca caaaccatta ccggacagat
     1621 tcgagcgctg gaagagcgcc tgcaaggcaa attatttaaa cgcaagggac gtggtctcga
     1681 acccagcgag ctgggagaac tggtctatcg ctatgccgat aaaatgttca ccttaagcca
     1741 ggaaatgctg gatattgtga actatcgcaa agaatccaat ttattgtttg acgttggtgt
     1801 ggctgatgca ctttccaaac gcctggtcag tagcgtactt aacgccgcag tggtagaagg
     1861 cgagcccatt catcttcgct gcttcgaatc cacccacgaa atgctgctgg agcaattaag
     1921 tcagcataaa ctggatatga tcatttctga ctgtccgata gactctacgc agcaggaagg
     1981 cctgttctcc gtgagaattg gcgaatgtgg cgtgagtttc tggtgtacaa atccaccacc
     2041 agaaaaaccg ttcccggctt gtctggaaga acggcgactt ttgattcctg ggcgacgttc
     2101 aatgttaggg cgcaaattgc ttaactggtt taactcccag ggattaaacg tagaaatcct
     2161 cggcgagttt gatgatgccg cgttgatgaa agcttttggt gcgatgcaca atgcaatctt
     2221 cgttgcccca acgctttatg catatgactt ttatgccgat aaaactgtcg tagaaattgg
     2281 tcgcgtcgag aatgtgatgg aagagtacca tgctattttt gctgagcgga tgattcagca
     2341 cccggcggta cagcgaatct gcaatacgga ttattctgcg ctttttagtc cagcggcgcg
     2401 ttaatcggca cctcccccca aagttaaggt gggggagata gattagttgt acataaccac
     2461 gattttgact cggctcatta tttgcccgct tgagacatta tttccatatg tacgcgggcg
     2521 aataaataga ggaatctgat tacttccttc atggggatgc tgaaaagagt agtaattgct
     2581 tttaccatac tggatttcat tcgtaccatc attggagagt tgaaagccta tttgacccgc
     2641 accgtcctca ctgttttcta actcattttt taatatgctg gttcctgact cctgtatcgt
     2701 cactggttca aagtaatatt caacatcact gggttcaaaa actaattcac cttgctcgcc
     2761 ctggatgcag tcaaatctta tatgatgaaa ctgataattt tcattattga tac
//
LOCUS       DAZGJW010000323         2799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZGJW010000323 DAZGJW010000000
VERSION     DAZGJW010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.326"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            109..2388
                     /gene="ydeP"
                     /locus_tag="ACHKIK_005211"
     CDS             109..2388
                     /gene="ydeP"
                     /locus_tag="ACHKIK_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO9086157.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLSWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKWMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2636..>2799
                     /gene="safA"
                     /locus_tag="ACHKIK_005212"
     CDS             2636..>2799
                     /gene="safA"
                     /locus_tag="ACHKIK_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO9086158.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDL"
BASE COUNT          738 a          658 c          682 g          721 t
ORIGIN      
        1 agtagtcccc tactcttata tatccatgtt ggcgataatc cctttgtatg tactgtgcat
       61 catcgctatt acaaatccta ataattcatt tccacacagg ataagtagat gaagaaaaaa
      121 attgaatcct accagggtgc tgcaggtggt tggggtgctg ttaaatccgt agcgaatgca
      181 gtacgtaagc agatggatat acgccaggat gttattgcca tgtttgacat gaataagcca
      241 gagggctttg actgtccggg ttgtgcatgg ccagatccta agcacagtgc gtcattcgac
      301 atttgtgaaa acggcgcaaa agcaatcgcc tgggaagtca cggataagca ggtaaatgcc
      361 tctttctttg ctgagaatac ggttcaatca ttactttcct ggggagatca cgagcttgag
      421 gctgcgggac gtctcactca gcctttgaaa tatgatgccg tcagcgactg ttacaagcca
      481 ttaagctggc aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc
      541 aatcaggttg aattctatac ttcgggccgc acttccaatg aagctgcctt tctttatcag
      601 ctttttgccc gtgaatacgg gagcaataac tttcccgact gctccaacat gtgccatgaa
      661 ccgacaagcg tgggtttggc agcgagtatc ggtgtaggta aagggaccgt gttgctggaa
      721 gactttgaga agtgcgattt agtcatttgc attgggcata accctggtac aaaccaccct
      781 cgcatgctga cttcgttgcg cgctttagtg aaacggggag cgaaaatgat cgccatcaat
      841 cctctacagg aacgtggcct ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg
      901 acgaactctg agactcagtt ggccagtgcc tactacaacg tgcgcattgg tggcgatatg
      961 gcgttgctca agtggatgat gcgcctgtta attgagcgcg atgatgctgc aagtgccgca
     1021 ggtcggccct cattgcttga tgacgaattt attcaaacgc ataccgtcgg ctttgacgag
     1081 ctacgccgtg acgttctcaa ttccgagtgg aaagatatcg aacgtatttc tggactaagt
     1141 cagacacaaa tcgccgaact ggctgacgca tatgccgctg ccgaacgcac cattatctgt
     1201 tacggaatgg ggatcactca gcacgaacat ggtacccaga acgtacagca actggtcaat
     1261 ttgctgttga tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc actacgtggt
     1321 cactctaatg tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt
     1381 ctggctcgcc tgggtgagcg ctatggcttc accccacctc atgcacctgg acatgctgca
     1441 attgccagca tgcaagcaat atgtacgggg caggctcgag cattgatctg catggggggc
     1501 aactttgcac tggcaatgcc agatcgggaa gcgagcgctg taccgttaac gcaattagat
     1561 ttggcggtac acgtagccac taagcttaac cgctctcatc tgctgaccgc acggcatagc
     1621 tatatcctgc cggttctggg gcgtagcgag attgatatgc aaaaaagcgg tgcgcaggcg
     1681 gtaaccgttg aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc
     1741 ggtgtaatgc tgaaatcaga gtgtgctgtg gtcgcgggaa tcgcccaggc aacactaccc
     1801 cagagcgtgg tagcctggga gtatctggtg gaagattatg atcgcattcg caatgacatt
     1861 gaagctgtgc tgccagagtt cgccgactat aaccagcgca tccgtcatcc cggtggtttt
     1921 cacctgataa atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc
     1981 attaccagca aagggctgtt agaagatccc tcttcagcgt ttaacagtaa gctggtcatg
     2041 gcgacagtac gcagccacga tcagtacaac acgacgattt atggtatgga tgatcgctat
     2101 cgaggggtat tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc taaaatttgc
     2161 cgtgtaaaaa acggcgaaag agttaatctt attgcgctta cgccagacgg taagcgcagc
     2221 tcacgccgca tggatagatt aaaagtggtc atttacccta tggctgaccg ctcactggtg
     2281 acctattttc cagaatcgaa tcacatgcta actcttgata accacgatcc attaagtggc
     2341 attcctggtt ataaaagtat tccggttgaa ttagaaccat caaattaatt cgcctcctca
     2401 tttcttctgc tgtcatccgc acagcagaag aatttctcat tgactattat cgcgcaattt
     2461 gctcacattg attaaattaa accacacact ataagatata aacctctgcc tacagctgta
     2521 agaaactccg ctcagtactg aagtgccagt cctatttcct cttttctcca gcctgttata
     2581 ttaagcatac tgattaacga tttttaacgt tatccgctaa ataaacatat ttgaaatgca
     2641 tgcgaccaca gtgaaaaaca aaatcacgca aagagacaac tataaagaaa tcatgtctgt
     2701 aattgtggtt gtcttattac tgacccttac gttgatagcc attttttcgg caattgatca
     2761 gctgggtatt tcagaaatgg gtcgcattgc aagagatct
//
LOCUS       DAZGJW010000324         2795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZGJW010000324 DAZGJW010000000
VERSION     DAZGJW010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.327"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..583
                     /locus_tag="ACHKIK_005213"
     CDS             <1..583
                     /locus_tag="ACHKIK_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9086159.1"
                     /translation="PSTVDPSNFDGSTVDNKLPIRGAMIDPDPSVLKPEPSDKRSSCP
                     DASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRIVSLYEQAASDD
                     GEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVKNIMSPAKLREK
                     WDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            590..1331
                     /locus_tag="ACHKIK_005214"
                     /pseudo
     CDS             590..1331
                     /locus_tag="ACHKIK_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1357..2118
                     /locus_tag="ACHKIK_005215"
     CDS             1357..2118
                     /locus_tag="ACHKIK_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO9086160.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGEAGVTEGQALQPEAPDVVTEEVAPKVTADMMVEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMSGGNLPADPKAAPVTKNDGKA
                     FPQPAGAALPVREAATQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRA
                     KGQVQKWERVCAALRELNKCRDILRDITATREQQR"
     gene            2151..2432
                     /locus_tag="ACHKIK_005216"
     CDS             2151..2432
                     /locus_tag="ACHKIK_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9086161.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            2429..2656
                     /locus_tag="ACHKIK_005217"
     CDS             2429..2656
                     /locus_tag="ACHKIK_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086162.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            2649..>2795
                     /locus_tag="ACHKIK_005218"
     CDS             2649..>2795
                     /locus_tag="ACHKIK_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086163.1"
                     /translation="MSEIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYV"
BASE COUNT          732 a          595 c          873 g          595 t
ORIGIN      
        1 cccctcaacg gttgacccgt caaattttga tggatcaact gttgataaca aactgccgat
       61 taggggggcg atgattgacc ccgatccgtc agtattaaaa cctgagccgt cagataaaag
      121 atcttcttgt ccggacgctt cgcaaccgga cccgcagacg gctgaacagg attttttaac
      181 ccgacaccct gacgcggttg tgttcagtgc gaaaaaacgc cagtggggaa gtcaggaaga
      241 tttggtgtgc gcacagtgga tctggggacg aatcgtgagt ctttacgagc aggcggccag
      301 cgatgatggc gagatcacga gaccgaaaga acccaactgg actgcatggg ccaatgacgt
      361 gcggacaatg cggatgctgg atggcagaac tcacagacaa atttgtgaaa tgtttgggcg
      421 tctccagcgg gattcgttct gggtaaaaaa catcatgagt ccggcaaaac tccgggaaaa
      481 atgggatgaa ctggttatcc gcctggggcg ttcgcccgcg cagcgttgcg tgaatcacat
      541 ttctgaaccg gacactgaaa taccgccggg attcaggggg tgacgtgtca tgaaaaacat
      601 tgcggcagtt ggggttcttg aacgtattcg cagacttgca ccacaggggg gcggttccac
      661 cgtaccggac ggtggaggag tggcgggaat ggcaacttgc tgaaggacga aaacgcagcg
      721 aggagattaa ccgcctgaat catcaggtgc gggttgaaaa aatcctgaac cgtgcgggca
      781 tccagccgct tcacaggaag tgctcattcg ggaactaccg ggtgcagaac gacggtcagc
      841 gccatgctct gagccaggcg aaatccattg ccgatgaatt gatgaccgga tgtacaaact
      901 tcgtgttcag cggtaaacct ggtaccggta aaaatcacct ggcagcagcg attggcaatc
      961 ggctgatggc gaaggggaga agcgtgatta tcgtcaccgt gtccgatgtc atgagcgtgt
     1021 tgcatgacgg ctacgacaac ggccagtccg gggaaaaatt tttacaggag ctttgtggag
     1081 ttgaccttct ggtccttgat gaaattggca tgcagcggga tacgcgcaac gagcaggtca
     1141 cgctgaacca gatagtcgac cgcagaacgg cttcgatgcg tagtgtcgga atgctgacga
     1201 acctgaatca cgcagcgatg agcacactcc tcggagatcg ggtgatggac cgtatgacca
     1261 tgaatggtgg tcgttgggtg aattttaact gggagagctg gcggtcaaac gttggacgtc
     1321 agggtatgtg agaatttttg acgaggtaaa ttttcgatgg aaactgtatt gcatgcactg
     1381 aaagcgatgg gaaaagccaa ttctgttgaa ctggcggcgc ggcttgatat cagccgtgaa
     1441 gaagttctta acgaactgtg ggaactcaaa aaaaatggcg ttgttgataa aacgggtcac
     1501 acctggtttc tggctgtcga aggtgaagcc ggggtaaccg aagggcaggc actacaacct
     1561 gaagcgccgg atgtggtaac cgaagaggtc gctccaaaag ttaccgcaga catgatggtt
     1621 gagtttatcg gtcaggatgg tgctaaaacg tgtgaggaac tggcgggtaa gttcggcgtc
     1681 agtactcgca aggttgcttc cacgctggcg gtggtaaccg caacggggcg gctggcacgc
     1741 gttaatcaga acggtaaatt tcgttactgc atgtcggggg gtaatttacc agcagatccg
     1801 aaagccgcgc cggtaacgaa aaatgatggt aaggcctttc ctcagccagc aggtgctgcg
     1861 ttaccagtcc gggaagccgc aacacaggaa gaaataaaaa cggaaagtgt ggcggtcaca
     1921 gtgcagtcac agccgtcgtt caccagaaag catccggatg gtctgatttt accatcgctg
     1981 catgtggcta accgcgagct gcgccgggca aaaggtcagg ttcagaagtg ggagcgagtc
     2041 tgcgccgcgc tgcgggagct gaacaagtgc cgggatattc tccgggatat taccgccacc
     2101 agagaacagc agcggtgagt gggtggaaga cgtggtgccg ggcggaaatc atgatactcc
     2161 ggcagtgtgc gggaacgatg aaggtaaaaa gcgttggcgc acttatcgga cgaactgaag
     2221 cggcagtgag aacgaaggca cgggagctgg gcatcagcat gatgttacgt ggtgattttc
     2281 acccgtcggc aaaatattct cagcgtgata ttgagctggc gcggcaactg catcagagag
     2341 gcatgcaaag aagggaaatt gccagaaaat taggcatgcc gctgcgcata gtgaataact
     2401 acgtttattt cgacaggagg gtgtctgcgt gaaaatcctg tatcaggatt acggcccggt
     2461 ggggcaggtg gttatcagca gtactgtaat ggagtttcgg aagcataacc gtgtggtgga
     2521 tgctgtgctg ttaacctgtc cggggatatc ggcgagtcgt gcaggtgtgt ttattatgaa
     2581 gacgaaatta tatggcagta aggcgtggat aaagaaggcg tatcgtgtag cgttgcagga
     2641 ggttaacagt gagtgaaatt aaagaaatgc cggtagttcg tgacggatat ggctactgga
     2701 cacatcctga atatgaaaaa ttctgtgatg gtcgggaata tatttcaacg gaagagttta
     2761 acgcctggat ggaggaaaat aatcttcaat acgtc
//
LOCUS       DAZGJW010000325         2779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZGJW010000325 DAZGJW010000000
VERSION     DAZGJW010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.328"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..164
                     /locus_tag="ACHKIK_005219"
     CDS             <1..164
                     /locus_tag="ACHKIK_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9086164.1"
                     /translation="LQRDSFWVKNIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPD
                     TEIPPGFRG"
     gene            205..627
                     /locus_tag="ACHKIK_005220"
     CDS             205..627
                     /locus_tag="ACHKIK_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO9086165.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLETKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKPKKPSAVDPDLIWSLPDG
                     EIRRYDRRLNIICLECRKSEVMQRVLAFYQGNFEEVVR"
     gene            624..878
                     /locus_tag="ACHKIK_005221"
     CDS             624..878
                     /locus_tag="ACHKIK_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086166.1"
                     /translation="MSESKCQVNGNKIEPCAALAKSLEHDAEYTMRKGLLIYKIWNES
                     LTRGPDFVMLRSGEFSKLPVRVSFCPFCGESLKTWENRNE"
     gene            871..1182
                     /locus_tag="ACHKIK_005222"
     CDS             871..1182
                     /locus_tag="ACHKIK_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086167.1"
                     /translation="MNEIKEIPVVRDEYGCWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGNGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            1488..2066
                     /locus_tag="ACHKIK_005223"
     CDS             1488..2066
                     /locus_tag="ACHKIK_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HFO9086168.1"
                     /translation="MRDIDVRKAVHAKILRDHHKDPDTLIIDEFTMNLGASRADIAVI
                     NGLIHGYELKSKSDNLLRLPAQVQHYSSVMDKVTLVVSDCHLYDALSIVPSWWGIKQV
                     TQGARQGIHLKTIRTSKLNPQVDKLSLTMLLWKDELLSLLSDVGELQNLKNKPKRVLW
                     SKLANSMDVGELREAVRVKLKARKEWRVAQQP"
     gene            complement(2026..>2779)
                     /locus_tag="ACHKIK_005224"
     CDS             complement(2026..>2779)
                     /locus_tag="ACHKIK_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HFO9086169.1"
                     /translation="PSNYKRAIDELMQNEICLRLTTLDLVNPQLITHYINHLGIPLSN
                     IDIIIDLRDELTEDKINSGELYTLAMGLINNLAHLNEYRKVILSGGSFPTDLSDISVG
                     LYSQPRIEWILWQSLMNRKELARNVIYSDYGVQHPDFNRLSTRFPSVSASVRYSGDND
                     FWVFRGRVANRFGYEQYGKHSEDILAHREYSGPTFCAGDRDIEYYANEYQAYKANPSG
                     NYKFGSPEVWRRIGQNHHITKVVEQLATLYGL"
BASE COUNT          830 a          533 c          654 g          762 t
ORIGIN      
        1 gtctccagcg ggattcgttc tgggtaaaaa acatcatgag tccggcaaaa ctccgggaaa
       61 aatgggatga actggttatc cgcctggggc gttcgcctgc gcagcgttgc gtgaatcaca
      121 tttctgaacc ggacactgaa attccgccgg gcttcagggg gtaagtgtta atttctggtc
      181 atgaggtaat tttcaggagg gcttgtggca aaagttttta cacaagaaga gcgggaaaaa
      241 attaaagggc aggttgttga actcgtacgc cagagtgggc gcgagacgtt acgacaactg
      301 gaaactaaaa ctggggcaac aagatatctg atgagcgttc tggccagaga gctggttgcc
      361 agtggcgatg tatacaactc tggttacggg ttattcccgt ctgaacaggc gcgtaaggac
      421 tggcaaaatg cccgtaaaaa gctctcaagg gcaaagccga agaaaccatc tgcggttgat
      481 ccggacctta tctggtcatt accagatggc gaaatacgcc gctacgacag gcgtctgaac
      541 ataatctgtc tcgagtgccg gaagagcgaa gttatgcagc gcgtactggc gttttatcag
      601 gggaattttg aggaggtggt gcggtgagtg aatcaaaatg ccaggttaat ggcaacaaga
      661 tagaaccatg tgcagcactg gcaaagtccc ttgagcatga tgctgaatac acgatgcgaa
      721 aaggtctgct gatatacaaa atctggaatg agagtttaac tcgcggtcct gattttgtga
      781 tgttgcgttc cggtgaattt tctaaattac cagttcgggt ttcattttgt ccgttctgtg
      841 gtgaaagtct gaaaacgtgg gagaacagaa atgaatgaaa ttaaagaaat accagtagta
      901 cgtgatgaat atggctgctg gacgcatcct gaatatgaaa aattctgtga cggtcgggaa
      961 tatatttcaa cggaagagtt taacgcctgg atggaggaaa ataatcttca atacgtcctc
     1021 tgcttcagag atgaaggatg tgctgacctt gatgcgtgtg atgctgatat ttctgcatgg
     1081 gaaccggaac gaccagaggg caatggatgg tttattggtt caatacatga caccgaagat
     1141 ggcccggttt gtgtatggct gagaaataag gccgaagcat aaaggctata aaccgactaa
     1201 caactaaata ctgaagattt aaatcagaaa cgatttttat taaaccctta accggaggga
     1261 ttctgcaccc tcagaacatc aggaggccgt ctgaaagggc ggaacagata atgcttacgt
     1321 tgaaacattt tatcgacata ccaacatggt tagccgtcat tgcttttgtt aaaatacaca
     1381 tccacttttc tgtgcaatgt ttaaccactg gtcatatcaa atggcattca tgcgaaccat
     1441 gatatagaat catggcttga gagagtcgat gaaagcgcaa ctatggtatg agagacattg
     1501 atgtaagaaa ggctgtgcat gccaagattc tgagagatca tcataaagat cctgacaccc
     1561 taatcattga tgagtttacg atgaatctag gggctagcag agctgatata gcagtgatca
     1621 atgggcttat acatggttat gagttgaaga gcaagagtga taacttgctc agattaccag
     1681 cgcaggtgca acattactca tcagtgatgg ataaagtaac tttggttgtc tctgattgcc
     1741 atctttatga tgctttaagc atagttccat catggtgggg gataaagcaa gttacgcaag
     1801 gtgcacggca aggtatccat ttaaaaacaa ttcgaactag caagttgaat ccacaagtgg
     1861 acaaactttc cttaacaatg cttctttgga aagatgaatt gctttcccta ttaagtgatg
     1921 taggggagct acagaatttg aaaaataaac ctaaacgcgt cttatggtca aaactcgcca
     1981 atagtatgga tgttggcgag cttcgtgaag ctgttcgagt taaacttaaa gcccgtaaag
     2041 agtggcgagt tgctcaacaa ccttagttat gtgatggttt tgcccaatcc tacgccatac
     2101 ctctgggcta ccaaatttat agttaccaga gggattggct ttgtaggctt gatactcgtt
     2161 tgcataatat tctatgtctc tatctcccgc acagaatgta ggccctgaat attctcgatg
     2221 agcaagaata tcctcactat gtttaccata ttgttcataa ccaaagcgat tagctactct
     2281 tcctcgaaat acccaaaagt cattatctcc agagtatctg acgctggcag atacgctagg
     2341 gaatcgcgtc gaaagcctat taaagtcggg gtgctgtact ccataatcac tataaatcac
     2401 atttctggca agttcttttc tattcattaa actctgccat aaaatccact cgattcgagg
     2461 ttgagaatat agaccaacag aaatatcact gagatctgta ggaaatgaac ccccagaaag
     2521 aatcactttt ctgtattcat tgaggtgcgc cagattgttt atcaatccca ttgccaaagt
     2581 atatagttcg ccggaattaa ttttatcctc ggttaactca tctcttaagt caataattat
     2641 atcaatattt gataaaggaa ttcccagatg attaatgtaa tgcgttatta attgtgggtt
     2701 aaccagatct aacgtggtta atctcaagca tatttcattc tgcattaatt catcaatcgc
     2761 tcttttatag ttagatggg
//
LOCUS       DAZGJW010000326         2776 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZGJW010000326 DAZGJW010000000
VERSION     DAZGJW010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.329"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1528
                     /gene="escC"
                     /locus_tag="ACHKIK_005225"
     CDS             <1..1528
                     /gene="escC"
                     /locus_tag="ACHKIK_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFO9086170.1"
                     /translation="SFFIFTALFCCSAQAAPSSLEKRLGKNEYFIITKSSPVRAILND
                     FAANYSIPVFISNSVNDDFSGEIKNEKPMKVLEKLSRLYHLTWYYDENILYIYKTNEI
                     SRSIITPTYLDIDSLLKYLRDTISVNKNSCNVRKITTFNSIEVRGVPECIKYITSLSE
                     SLDKEAQSKAKNKDVVRVFKLNYASATDITYKYRDQNVVVPGVVSILKTMASNGSLPS
                     TGKGAVERNGNLFDNSVKISADPRLNAVVVKDREITMDIYRQLISELDIEQRQIEISV
                     SIIDVDANDLQQLGVNWSGTLNAGQGSIAFNSSTAQANLSSSVISNANNFMIRVNALQ
                     QNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRVTPRILD
                     DNSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSILPEVQNSEMTTEATLSAGES
                     LLLGGFIQDKESSSKDGIPLLSDIPVIGNLFSSTTKQKHSVVRLFLIKATPIKSASSE
                     "
     gene            1542..1997
                     /gene="cesD"
                     /locus_tag="ACHKIK_005226"
     CDS             1542..1997
                     /gene="cesD"
                     /locus_tag="ACHKIK_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFO9086171.1"
                     /translation="MSRKFSSLQDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNRKMAKKAFKACLMWCNEQEKYATYKENIKKLLGNTD"
     gene            complement(2383..>2776)
                     /gene="grlA"
                     /locus_tag="ACHKIK_005227"
     CDS             complement(2383..>2776)
                     /gene="grlA"
                     /locus_tag="ACHKIK_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HFO9086172.1"
                     /translation="GDYVIPDSVKNYDGEPLYILVSLWCKLQEKWISRNDIAEAFGIN
                     LRRASFIITYISRRKEKISFRVRYVSYGNLHYKRLEIFIYDVNLEAVPIESPVSTGPK
                     RKNYRVGNGIVGQSNIWNEMIMRRKKEN"
BASE COUNT          912 a          539 c          508 g          817 t
ORIGIN      
        1 aagttttttt atttttacag cactattttg ctgcagtgct caggctgccc cgtcctctct
       61 ggaaaaaaga ttaggcaaga acgagtattt tattattaca aaaagctctc cggtacgtgc
      121 aatcctcaat gattttgccg ctaattatag catccctgtt ttcatcagta attctgtgaa
      181 tgatgatttc tctggtgaaa taaaaaatga aaaaccaatg aaagtactgg aaaaactgtc
      241 caggctctat catctcacat ggtattatga tgagaacatt ttgtatattt ataaaacaaa
      301 tgaaatatca agaagcataa ttacgccaac atatctggat attgactcac tgttaaaata
      361 tctgcgtgat acaatctcag taaataaaaa ctcctgtaac gtgagaaaaa tcacaacatt
      421 taactcgatt gaagttcgtg gcgttcctga atgcataaaa tacattacat ctttatctga
      481 aagtcttgat aaagaagcac agtctaaagc aaaaaataaa gatgtcgtta gagtatttaa
      541 attaaactat gcatcagcga cagatataac gtataaatac cgcgaccaaa atgttgtcgt
      601 gcctggcgtc gtctccatcc tgaagacgat ggcaagtaat ggctcacttc ccagcacagg
      661 caaaggtgct gtagaaagaa acggcaacct gtttgataac agtgtaaaga tttccgcaga
      721 tccacgtctt aatgcagttg ttgtaaaaga tcgtgaaata actatggaca tatatcggca
      781 attaatttct gagcttgata ttgagcaaag gcaaattgag atttctgttt caattattga
      841 tgttgatgca aatgatcttc agcaactggg agtaaactgg tccggtacat taaatgcagg
      901 gcaaggttct attgccttta acagctcaac ggcgcaagct aatctcagtt cttctgtgat
      961 aagcaacgcc aataatttca tgatcagagt gaatgcgtta caacagaact cgaaagcaaa
     1021 aatactctct cagccatcaa ttataacctt aaataatatg caggcgattc ttgataaaaa
     1081 cgtaacgttt tatacaaaag tttccggaga aaaggtggca tctctggaaa gtattaccag
     1141 cggaacatta ctgagagtaa caccacgtat acttgatgac aactcgaata gtcttaccgg
     1201 aaaacgtcgg gaacgagttc gtttgttact tgatattcag gatggcaatc aatcgactaa
     1261 ccaaagtaat gctcaggatg ccagcagcat cttacctgaa gtacaaaatt cagaaatgac
     1321 aacagaagcg acattaagcg ctggagaaag tctgctgctt ggcggattta tccaggataa
     1381 agagagttca agtaaagatg gtataccgtt gttaagtgat attcctgtta ttggtaactt
     1441 attctccagt acaacaaaac aaaaacacag cgttgttcga ctctttttga ttaaagcgac
     1501 cccgataaaa tcagcatcta gcgaataata aatgaggctt tatgagcagg aaatttagct
     1561 ctctacagga tatctatgat ttctaccagg atggtggtac gttagcatca ctgacaaatt
     1621 tgacgcaaca agatctcaat gatcttcatt cttatgccta tacagcatat caatctggtg
     1681 atgttataac cgcaagaaat ctatttcatc tgctaacata tctggagcac tggaattatg
     1741 actacaccct atctctgggg ttgtgtcatc agcgtttatc aaatcatgaa gatgcacaac
     1801 tgtgtttcgc tcgctgtgca actttagtta tgcaagatcc cagggcatct tattattctg
     1861 ggattagcta cttattggtc ggaaatcgga aaatggccaa aaaagccttt aaggcttgtt
     1921 taatgtggtg taatgaacaa gaaaaatacg ctacatataa agaaaatatt aaaaaattgt
     1981 taggtaatac agattaaaaa ctaccatctg ccataccaat ccccaggctg tctggggatt
     2041 ttctatcgga aataataaag ctgctttggt gaagtgcgcc caacgttgag caccgaccgc
     2101 tggcagattt atctacagga gtgggttact ccattaacca ggattttatg cgcctgaacc
     2161 atcttctgct gtactcaatg tcatttccgc ttatagaata agcagtatat atcttaactt
     2221 agcatcagaa atctgactgt ttcaatcatc tatagcccag agtgtatttg tcaggaaatg
     2281 actttcaaac tttggcagag aaaaaggctt atcctggaaa acaaagccct tgaatagagc
     2341 tttattttta ttcttctata aaatatactc aaaaaaatac gtttaattct cctttttccg
     2401 cctcatgatc atttcgttcc agatattaga ctgccccaca ataccattac cgactcggta
     2461 gttttttctt tttggtccgg ttgataccgg actttctatc ggaacagcct cgaggttaac
     2521 atcataaatg aaaatctcaa ggcgcttata atgcaagtta ccataactaa catatctgac
     2581 acgaaatgaa attttttctt ttcttctcga tatataagtt ataataaatg atgctctcct
     2641 caggtttata ccgaatgctt cggcaatatc attgcgagaa atccatttct cctgcaattt
     2701 acaccaaaga gaaaccaaga tatacagagg ctcaccatcg taattcttta ctgagtcagg
     2761 aattacatag tcgcca
//
LOCUS       DAZGJW010000327         2773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZGJW010000327 DAZGJW010000000
VERSION     DAZGJW010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.330"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(145..>2773)
                     /gene="eae"
                     /locus_tag="ACHKIK_005228"
     CDS             complement(145..>2773)
                     /gene="eae"
                     /locus_tag="ACHKIK_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="intimin type beta"
                     /protein_id="HFO9086173.1"
                     /translation="YTLKTGETVANISKSQGISLSVIWSLNKHLYSSESEMMKAGPGQ
                     QIILPLKKLSVEYSALPVLGSAPVVAAGGVAGHTNKMTKMSPDATKSNTTDDKALNYA
                     AQQAASLGSQLQSRSLNGDYAKDTALGMASSQASSQLQAWLQHYGTAEVNLQSGNNFD
                     GSSLDFLLPFYDSENMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYNVFIDQDF
                     SGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRFNGYLPS
                     YPALGAKLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYRHGTGNE
                     NDLLYSMQFRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKKQDILSL
                     NIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHGGSQSAQDYQAIL
                     PAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLPNGQVVDQVGVTDFTADKTSAKA
                     DGIEAITYTATVKKNGVAQANVPVTFSIVSGTATLGANSARTDGNGKATVTLKSATPG
                     QVVVSAKTAEMTSPLNASAVIFVDQTKASITEIKADKTTAKADGSDAITYTVRVMKEG
                     APVVDQKVTFSKDFGTLNKTEATTDQNGYATVKLSSNTPGKAIVSAKVSGVGTEVKAT
                     TVEFFAPLSIDGDKVTVIGTGITGALPKNWLQYGQVKLQATGGNGKYTWKSSNTKIAS
                     VDNSGVITLNEKGSATITVVSGDNQSATYTINAPGSIVIAVDKNTRVTYFDAENKCKT
                     NSANLAQSKELLANIYSTWGAANKYPYYSGSKSLTAWIKQSSSEQSSGVSSTYDLVTK
                     NQLINVGVNNKNAFSVCVK"
BASE COUNT          776 a          654 c          535 g          808 t
ORIGIN      
        1 ctctatcaaa caagatacac gaaaaactta ttccgaaatg agacattgat tttaacatga
       61 aacgtttatt aaaagattca agtaggtaca ataaaaaatc ccggaaaatg aattttattt
      121 tccgggattt gagatgtaat taaattattt tacacaaaca gaaaaagcat tcttattgtt
      181 tactccaaca ttgatcaact ggttcttcgt aaccaaatca tatgtgcttg atacacctga
      241 tgactgttca gaagaggatt gtttaatcca agcagtcaat gatttagaac cagaatagta
      301 aggatattta tttgcagcac cccatgttga atagatattg gccaatagtt cttttgactg
      361 tgctaaattt gcgctatttg tcttacattt gttttcggca tcaaaatacg taactcgagt
      421 atttttatcc acagcaatta caatactacc cggtgcatta attgtgtatg tcgcactctg
      481 attatcacca gatactacag taattgtggc actccctttt tcatttaagg ttatcactcc
      541 cgagttatca acagaagcaa ttttagtatt actggatttc catgtgtatt ttccattgcc
      601 ccctgttgcc tgtagcttaa cctgaccata ctgtaaccag ttctttggca gagcccccgt
      661 gataccagta ccaattacgg tcactttatc accatcaata ctcaacgggg caaaaaactc
      721 aacggtagta gccttaactt ctgtacctac tccactcact tttgcactaa caatggcctt
      781 gccaggagta ttggatgata attttacagt agcataacca ttctgatcgg ttgttgcttc
      841 agtcttattc agggtcccaa aatccttaga aaaggtcact ttctgatcta ctacgggtgc
      901 cccctccttc atcactctga cagtataggt aatcgcatca gaaccatctg ccttcgctgt
      961 tgttttatca gccttaatct cagtaatact ggccttggtt tgatcaacaa atataaccgc
     1021 gctggcatta agtggcgaag tcatctccgc ggttttagca gacacgacga cctgtcctgg
     1081 cgtagccgac ttcagcgtta cggtcgcctt accgttacca tccgttctgg cactatttgc
     1141 cccaagagtt gcagtcccgg atacaatact aaatgttaca gggacattag cctgagctac
     1201 accattcttt ttaaccgtcg cggtataggt aatagcttct atgccatccg ctttagccga
     1261 tgttttatca gccgtaaagt ccgttacccc aacctggtcc acaacctgcc cattcggtaa
     1321 aacggtaata gtgagctgta cattattaga actattacca tttcggtcat aggcgcgagc
     1381 ggtcacttta taaatattgc tgccgccttg cacataagca ggcaaaatag cctggtagtc
     1441 ttgtgcgctt tggcttccgc catgctgaat ctgaccgccc tgactgcgta atgcgctatc
     1501 atcccagacg atacgatcca gaccgtattt gctcttaacg atcaattgaa tcttctgcgt
     1561 actgtgttca gtaccattaa tatcatgcgg aatattcaga gaaagaatat cctgcttttt
     1621 gtactccaga ataatattgt tattacgctg aaccagatcg taacggctgc ccgataatgt
     1681 tcttaactcg ttaacatact gtggctcgat ttgctgagac cacggtttat caaactgata
     1741 acggaactgc attgagtaaa ggagatcatt ttcattaccc gtaccatgac ggtaatcgat
     1801 ccccatcgtc accagaggaa tcggagtgta gtttacacca acggtcgccg cgccaggatt
     1861 cgactgcaac ttatcggaat taaacaaagc aacattatca ccataatact gttcgtacat
     1921 cagtttggcg cctaatgccg gatatgatgg taaatagcca ttaaagcgga tatcaaaacc
     1981 atttgccggg cgctcatcat agtctttctt attgtatgac tcatgccagc cgctcatgcg
     2041 gaaatagccg ttaacgctac ttttgaaata gtctcgccag tattcgccac caatacctaa
     2101 acgggtatta tcaccagaaa aatcctgatc aatgaagacg ttatagccca acatattttc
     2161 aggaaggaaa aaacgctggc cagcacctaa atttgccgta aagcgggagt caatgtaacg
     2221 cgcaccgacc tgaccaaatg ccagcatgtt ttcggaatca tagaacggta ataagaagtc
     2281 cagtgaacta ccgtcaaagt tattaccact ctgcagatta acctctgccg ttccataatg
     2341 ttgtaaccag gcctgcaact gtgacgaagc ctggctgctg gccataccaa gagcggtatc
     2401 tttcgcgtaa tcgccgttca gtgagcgcga ctggagctgg ctaccaaggc tcgcggcctg
     2461 ttgtgccgca taatttagag ccttgtcatc ggtcgtgttg cttttagtcg cgtccgggga
     2521 cattttagtc attttattcg tatgaccagc gacaccacct gcagcaacaa caggtgccga
     2581 acctaagaca ggtaaggcac tatattcaac agacagtttt ttgagtggca aaatgatctg
     2641 ctgaccaggt ccagccttca tcatttcgct ttcggaactg tataaatgtt tattcagtga
     2701 ccaaattacc gataaactga taccctgtga tttagaaata ttggcaacag tttcacctgt
     2761 ttttaacgta taa
//
LOCUS       DAZGJW010000328         2772 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZGJW010000328 DAZGJW010000000
VERSION     DAZGJW010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.331"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            171..896
                     /locus_tag="ACHKIK_005229"
     CDS             171..896
                     /locus_tag="ACHKIK_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9086174.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            854..1531
                     /gene="bglJ"
                     /locus_tag="ACHKIK_005230"
     CDS             854..1531
                     /gene="bglJ"
                     /locus_tag="ACHKIK_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO9086175.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMTRISFAAVIFSFSAMRSERREGLSCLTELAIKLPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1569..2357)
                     /gene="fhuF"
                     /locus_tag="ACHKIK_005231"
     CDS             complement(1569..2357)
                     /gene="fhuF"
                     /locus_tag="ACHKIK_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO9086176.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLRAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2498..2734
                     /locus_tag="ACHKIK_005232"
     CDS             2498..2734
                     /locus_tag="ACHKIK_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO9086177.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLILTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMLMNLNQG"
BASE COUNT          736 a          602 c          699 g          735 t
ORIGIN      
        1 taaccttctg acacatgcag tggagttgtt gtgcagcagg agtatgctga tatgaaagtg
       61 aatgctaagg ataatttatt cgcttaatct attaatttgc tggaaacatt taaggaatga
      121 atatcctgaa taaaccatca taatttttag acagtggatg tggagggaat atgttgccag
      181 gatgctgcaa aaatggaatt gttatcagta aaatacctgt tatgcaagca gggttaaaag
      241 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
      301 ttaccttatt acaattacgc cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      361 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      421 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaactg ctcatgtgcc
      481 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      541 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgactgaaa
      601 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      661 tattaggtaa aggatggggc atcaaccaga tagcttcatt gcttaagaaa agtaataaaa
      721 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc aattcacagc aacgctgaaa
      781 tgtatgcatg gataaatagc gcgcagggtg caagagaact taacttgcct tctgtttatg
      841 gagatgccgc agaatggaac acagccgaat taagaagaga aatgtcgcac tcatagaaaa
      901 atgcgtcatg agtagtatcg gtattgagag tttattcaga aagtttgcgg gtaaccctta
      961 taagctccat acctatacca gtcaggagtc atttcaggat gccatgacgc ggatctcgtt
     1021 tgcggcggtc attttttctt tttctgccat gagaagtgag cgcagagagg gattatcttg
     1081 cctgactgaa ctggcgatta agcttccgcg tacccggcgt ttagttattg cggatgatga
     1141 tattgaagct cggctgattg gttcattgtc gccatcaccg ctggacggtg tattaagtaa
     1201 agcgtcaacg ctggagattt ttcatcagga actctttttg tcattaaatg gtgtacgtca
     1261 ggcgaccgac cgactgaaca atcagtggta cattaaccaa agccggacgc taagcccgac
     1321 ggagagagaa atattgcgct ttatgtcgcg tggttactca atgacacaaa ttgccgagca
     1381 gcttaaacgt aatatcaaaa cgatccgtgc acataaattt aatgtgatgt cgaaactggg
     1441 cgtcagttct gatgcagggt tgttggaggc cgcagatatt ctgttatgta tgcggcattg
     1501 cgaagcaagt aatgtgttgc atccctatta atccgcatga tgccgggttt acttccccgg
     1561 cagtgctttc atttcagcgt acaatcgcca cattgctgca catccggtaa gcgataacgc
     1621 tggcagcaag tgcggcgcac cagcaggccg tcgcgcagta ccacggtacg ccagagtgga
     1681 ttatcttcac cggtagtaag cgttttctca aaaaagaggg catggcgcag cgattcaacc
     1741 gtcgcctcgc cgagcagctg tttcatctca gtcagatacc agttgatcaa ataaccggta
     1801 ttactccaga taagtttgcc gttgatctct cccgtcgctt ctagtgcctg cacaaccgga
     1861 accagcgcct ggctgattaa cgtttccatt cgctgctgcg gcgaatgtgg tgttgcgttt
     1921 ttatcttcac acacatcgac ccagaaacag gcggcacggc cggtttcgtg aaactcaacg
     1981 tggaaatgtt ccggcgacac atctaacgct ttttcctgcg tcagcagcgc cagcattaat
     2041 ggtggcacca tcaggccgat ataccattgc gcccatagtg agatcagcgg cttgttctcg
     2101 cggatcatcg ttggttggtt gcgatagata tgatcggaat agaccgccag tagagaactt
     2161 agcgcattcg gtgatgacca ttgcgccagc gtcatggcgc gaagcggggc gggttcatcc
     2221 agacggataa actccagcaa atgttcacga tgttcggcga tcgtcgcccg tacggcttgt
     2281 gcaagcccag catcctgcgg ctggagatgc gttcgccaga tgacatcttc atagagcggt
     2341 gcggaacgat aggccataat cgggatagta atctaaatga taatgattgc taatcctagc
     2401 gataggttta cccgatagca agggatttct ctggcttgca aatgataaaa attatcatat
     2461 gatattggtt atcattatca atgaaagaga tgaaaccatg ttgcaacgta cgctgggcag
     2521 tggctgggga gtgttgctgc cgggattgct gattgcaggg ctgatgtatg cggatttatc
     2581 gccggatcaa tggcggattg tcattctgat gggattgata ttgacgccgg tgatgctgta
     2641 tcacaaacag ttacggcatt acgttttgct accatcgtgc ctggcgctta ttgctggcat
     2701 catgctgatg ctaatgaatt tgaatcaggg atgaaaaatc aaggaagaaa caagaaagga
     2761 agtaaagata at
//
LOCUS       DAZGJW010000329         2769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZGJW010000329 DAZGJW010000000
VERSION     DAZGJW010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.332"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(186..917)
                     /gene="dnaQ"
                     /locus_tag="ACHKIK_005233"
     CDS             complement(186..917)
                     /gene="dnaQ"
                     /locus_tag="ACHKIK_005233"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO9086178.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            982..1449
                     /gene="rnhA"
                     /locus_tag="ACHKIK_005234"
     CDS             982..1449
                     /gene="rnhA"
                     /locus_tag="ACHKIK_005234"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO9086179.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1446..2168)
                     /locus_tag="ACHKIK_005235"
     CDS             complement(1446..2168)
                     /locus_tag="ACHKIK_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO9086180.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            2202..>2769
                     /gene="gloB"
                     /locus_tag="ACHKIK_005236"
     CDS             2202..>2769
                     /gene="gloB"
                     /locus_tag="ACHKIK_005236"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO9086181.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYY"
BASE COUNT          728 a          682 c          653 g          706 t
ORIGIN      
        1 ccgcgacccc ctgcgtgaca ggcaggtatt ctaaccgact gaactaccgc tccgcgttgt
       61 gttccgttgg gaacgaggcg aatattacgg attgcctcac ccttcgtcaa cggtttttct
      121 catcttttga atcgtttgct gcaaaaatcg cccaagccgc tatttttagc gccttttaca
      181 ggtatttatg cccgccagag gcagcttccg cccttcttct ccaccagatc aagacgggct
      241 tcatgagctg caagctcttc atctgtcgca aaaacaacgc gtaacttact tgcctgacgt
      301 acaatgcgct gaattgttgc ttcaccttgt tgctgctgtg tctctccttc catcgcaaaa
      361 gccatcgacg tttgaccacc ggtcatcgcc agataaactt ccgcaaggat ctgtgcatcg
      421 agtaatgccc cgtgtagcgt tcgtttactg ttatctattt cgtagcgggc acataacgca
      481 tcgaggctgt tgcgcttacc aggaaacatt ttcctcgcca ccgcaaggct atcggtgacc
      541 ttacagaagg tattggtctt cggaatatcg cgcttaagca acgaaaactc gtagtccata
      601 aagccgatat cgaacgctgc gttatggatc accaactccg cgccgcggat atagtccatg
      661 aactcatcgg ctacttcggc aaacgtgggc ttatcgagca aaaattcatc ggcaatacca
      721 tgtacgccaa aggcttccgg atccaccagc cgatcgggtt tgagataaac atggaagtta
      781 ttgcccgtca ggcgacggtt caccacttca acggcaccaa tctcaatgat cttgtggcct
      841 tcatagtgcg caccaatctg gttcataccg gtggtttcgg tatcgagaac gatctggcgt
      901 gtaattgcag tgctcatagc ggtcatttat gtcagacttg tcgttttaca gttcgattca
      961 attacaggaa gtctaccaga gatgcttaaa caggtagaaa ttttcaccga tggttcgtgt
     1021 ctgggtaatc ctggacctgg gggttacggc gctattttac gctatcgcgg acgcgagaaa
     1081 acctttagcg caggctacac ccgcaccacc aacaaccgta tggagttgat ggccgctatt
     1141 gtcgcgctgg aggcgttaaa agaacattgc gaagtcattt tgagtaccga cagccagtat
     1201 gtccgccaag gtatcaccca gtggatccat aactggaaaa aacgtggctg gaaaacagca
     1261 gacaaaaaac cagtaaaaaa tgtcgatctc tggcaacgtc ttgatgctgc attggggcag
     1321 catcaaatca aatgggaatg ggttaaaggc catgccggac acccggaaaa cgaacgctgt
     1381 gatgaactgg cccgtgctgc ggcaatgaat cccacactgg aagatacagg ctatcaagtc
     1441 gaagtttaag cctgtggttt acgatattgc cgggtggctc caaccgcctg gcgaattcgt
     1501 ggcttgtttt tactctgttt catcggattt agcgttaaag gaatagtccg tttccgggca
     1561 acaataagtt gtaagcaacc aagcgcagga atatgtgcat tcaatagttt tccaccgtgt
     1621 ttgttccacg ggagaacgtg gaaacggctg gcgtgcagca cttcaaaatt caacaaagag
     1681 agccagtcca gttgccgcat cagagtaaac atccggctgt tatagggcga ggtttttcgc
     1741 aataccggca caagtttgcg taatcccatt aaactaatgg gattgaagcc actaatcacc
     1801 agccagccat catcaatcaa tacccgatcg gtttcacgca ataaacgatg cggatcggtg
     1861 caccacggca atgtatgtgc cagtagacaa acatcaacgg atttatcggc aaaaggaaga
     1921 tgaagtggat ccgcctgcac ctggacgggc attccttgcg cagaaacatt cacttgatga
     1981 gaaaccgcgc acgcttcgca attgatttct gcgcttaaat tgccaatctt aagcagatga
     2041 aaaccataca ttttagtgaa ccacgggttg agctggcgct ccagcgcctt gcgataaagc
     2101 tctccccagg gcaaatcgcc ccagcgatca ggagccacga cagtttgagg gactcttgcc
     2161 ggtttcatca caaccttccg tttcacacta agaggtaatc tatgaatctt aacagtattc
     2221 ccgcctttga tgacaattac atctgggttt tgaatgatga agcaggtcgc tgcctgattg
     2281 tcgatcccgg agacgcagag ccagtattaa acgccattgc cgccaataac tggcaaccgg
     2341 aggccatatt tctcacccac catcatcacg atcacgttgg cggcgtaaaa gaactggtgg
     2401 aaaagtttcc gcaaattgtg gtgtatggtc cacaagagac acaagataag ggaacaacac
     2461 aggtagtcaa agatggcgaa actgccttcg ttttggggca tgaatttagt gtaattgcca
     2521 caccgggtca cactttagga catatctgtt acttcagtaa accttatcta ttttgcggcg
     2581 acacgctgtt ttctggtggg tgtggtagat tgtttgaagg gacagcatca caaatgtatc
     2641 aatcacttaa taagttaagt gcgttacctg acgatacatt ggtatgttgt gctcatgaat
     2701 atacgttatc aaatatgaag tttgctttga gtattcttcc gcacgatttg tccataaatg
     2761 attattatc
//
LOCUS       DAZGJW010000330         2652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZGJW010000330 DAZGJW010000000
VERSION     DAZGJW010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.333"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..421
                     /locus_tag="ACHKIK_005237"
     CDS             <1..421
                     /locus_tag="ACHKIK_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO9086182.1"
                     /translation="ANQYVMAFYWPVSSLPQGVALTGDGGNNLLAAFYIQTDAKDLNV
                     MYHNAKVATNNLKLGTFGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPV
                     SAFAADKLHVTDITRNATYPVLIDSIAVEVNSTDTAA"
     gene            557..736
                     /locus_tag="ACHKIK_005238"
     CDS             557..736
                     /locus_tag="ACHKIK_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO9086183.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDVWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            777..1049
                     /locus_tag="ACHKIK_005239"
     CDS             777..1049
                     /locus_tag="ACHKIK_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO9086184.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            1126..1341
                     /locus_tag="ACHKIK_005240"
     CDS             1126..1341
                     /locus_tag="ACHKIK_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO9086185.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGK"
     gene            1346..1690
                     /locus_tag="ACHKIK_005241"
     CDS             1346..1690
                     /locus_tag="ACHKIK_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO9086186.1"
                     /translation="MKKKYELVVKGINNYPDKITVTVALEIGGYPSLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPIILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            1741..2274
                     /gene="rrrQ"
                     /locus_tag="ACHKIK_005242"
     CDS             1741..2274
                     /gene="rrrQ"
                     /locus_tag="ACHKIK_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9086187.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            2434..2571
                     /locus_tag="ACHKIK_005243"
     CDS             2434..2571
                     /locus_tag="ACHKIK_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086188.1"
                     /translation="MFDFSKLIREIRMMAEKLSTWKFILIWLVFVIMASGYFIGQIRW
                     W"
     gene            2573..>2652
                     /locus_tag="ACHKIK_005244"
     CDS             2573..>2652
                     /locus_tag="ACHKIK_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO9086189.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYR"
BASE COUNT          694 a          582 c          758 g          618 t
ORIGIN      
        1 tgcgaaccag tatgttatgg cgttttactg gccggtctct tcactgccac agggcgttgc
       61 cctgaccgga gacgggggga ataacctgct ggcagcgttc tacatccaga cagatgcaaa
      121 agacctgaat gtgatgtacc acaatgcgaa agtggcgaca aacaacctga aactgggaac
      181 ctttggcgca tttgataacg aatggcatac gctggctttc cgctttgccg ggaataacag
      241 ccttcaggtg acgccggtta ttgatggtca ggatggcaca ccgttcacgc tgacgcagtc
      301 accggtcagt gcatttgcgg cggataaact gcatgtgaca gacattacca ggaatgcgac
      361 ttacccggta ctgatagaca gcattgcggt ggaagtgaac agcacagaca ctgcggcatg
      421 ataaaaaaaa ccgccagcga caggaatgga cgctggcggt ggtgatacct atggagaaaa
      481 aataaaggaa cgatactttc gtactctggt ttttaatgaa aacagttctt attgtcaaca
      541 ataacggaaa gaaattatga catttctgaa ccagttaatg ctgtacttct gtacggtggt
      601 ctgtgtgctg tatctccttt cgggtggata ccgggccatg cgtgacgtct ggcgcagaca
      661 gattgacaaa agggccgctg agaaaatcag cgccagtcag tcagccggaa gcaaacccga
      721 agagccgctc atttagcggc aactttctta atcacatctt tcgacgagaa aatcccatgt
      781 cagaaattac atccctggtc actgctgaag cagtgaagga agtcctgcgc tctgaagaag
      841 tccggagcgc actgaaacaa aaacttcgcc ataacctgga agcgcgtctt gatgcagagg
      901 ttgatgccat tctggatgag ctgcttggtg tacaggcaga gccaccgact gaagcgggag
      961 ataccaccgc agagagcggt gaagttcagc ctgaatcacc ggtcgccgat gcgactgaac
     1021 ctcaacccga atcggtcatg atgctgtaac ggggagtcag ggccatcagt agttaactgg
     1081 tggctttttt attgttgtca gcttccggat aacgggagac ggggtatgta ccagatggaa
     1141 aaaatcacaa cgggtgtgtc atacaccacg tcagcggtgg ggacgggata ctggttactg
     1201 cagctgctgg acaaagtctc tccgtcccag tgggtggcaa tcggtgtgct ggggagtctg
     1261 ctgtttggcc tgctgacgta tcttactaac ctgtatttca aaatcagaga ggaccgtcgt
     1321 aaggcggcgc ggggaaagta aagcgatgaa gaaaaaatac gaactggttg ttaaagggat
     1381 aaataattac ccggataaga ttactgttac tgtggcactg gaaattggtg ggtatccgtc
     1441 actgttgttg ccagatgtgg cgattagtct tgaccgtact gaaggtgcca cgctggagtt
     1501 ttacgaagct gaggcgaaaa agcaggcgaa gcagtttttc atggatgttg ctgccgggtt
     1561 atgtgaaggg gatggtccgt tgccggaaaa gcggcccatc attttagagg cgcaggatgt
     1621 gttgataacc tacagaggaa aactaccggg aataattact ggttctctga agactccacc
     1681 gctggcctga agacttaata tatccaggga tttgaaatcg ataaaccctg ataaatatcc
     1741 atgaacgcaa aaatcagata cggcctgtcg gctgccgttc tggcgctgat tggtgcaggg
     1801 gcgtctgcgc ctgaaatcct cgaccagttt cttgacgaaa aagaaggtaa ccacaccacg
     1861 gcataccgtg atggtgcggg gatctggacc atctgccgcg gtgccatcct ggtggatggt
     1921 aaacctgtcg tcccgggcat gaagttgtcg aaggaaaaat gcgaccaggt taacgccatt
     1981 gaacgtgata aggcgctggc atgggtggag aaaaacatca gagtgccact gaccgaaccc
     2041 cagaaagcgg ggattgcgtc attctgtccg tacaacattg gccccggtaa gtgtttcccg
     2101 tcgacgtttt acagacggat taatgccggt gaccgcaggg gagcatgcga ggcgattcgc
     2161 tggtggatta aggacggtgg cagagactgc cgtattcgct caaacaactg ctacggtcag
     2221 gtctcacggc gtgaccagga gagcgcgctg gcgtgctggg gaattgacag ataagcagaa
     2281 tattttgctg aaaaatgcga tttgctcacg cggacggata acacgaaatc ctgcgaactg
     2341 acaaaaacta agtgaataaa agtaaaaacc ccgtttgttg gctgcaagcg gggttttgtg
     2401 tttcctgact ccggaaaagt caaaggagaa agtgtgtttg attttagcaa actgattcgg
     2461 gagattcgaa tgatggctga aaaattatcc acctggaagt tcatccttat ctggctggtg
     2521 tttgtgatta tggcttccgg ttatttcatt ggtcagatac gctggtggtg aaatgaaccg
     2581 cgtactgtgc gtggtcatca ttgccctgct ggtggcctgt ggtgcgctta gtctggggct
     2641 gaatcattac cg
//
LOCUS       DAZGJW010000331         2649 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZGJW010000331 DAZGJW010000000
VERSION     DAZGJW010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.334"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(60..1250)
                     /gene="aspC"
                     /locus_tag="ACHKIK_005245"
     CDS             complement(60..1250)
                     /gene="aspC"
                     /locus_tag="ACHKIK_005245"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO9086190.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMALLCEAI
                     VAVL"
     gene            complement(1435..2523)
                     /gene="ompF"
                     /locus_tag="ACHKIK_005246"
     CDS             complement(1435..2523)
                     /gene="ompF"
                     /locus_tag="ACHKIK_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO9086191.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          656 a          654 c          637 g          702 t
ORIGIN      
        1 atgttgcgtt tgtcatcagt ctcagcccgc ttttcagcgg gcttcattgt ttttaatgct
       61 tacagcactg ccacaatcgc ttcgcacagc agagccatgt tatctggtgt catcccggcc
      121 acatttacgc gaccagaagc aaccgcatat acgccaaact cttcgcgcag acgcagcact
      181 tgttcttttg tcaggccact gaaggagaac atgccgttct gtttgatgat aaagctgaag
      241 tcgcggtttg cgcctttttc ctgcagcgta ttgacgaaca actgacgcat acgctgaata
      301 cgctggcgca tatcagtcag ctcttgttcc caaatcgcac gtaacgcatc gttgctcagg
      361 atggtggcaa caacagaagc gccgtgtgct ggtgggttag agtagttagc gcgaatcgcc
      421 gctttcattt ggctgaatgc gcgatcaacg gtttcactgt cggcagcaac cagagtacaa
      481 gcgccaacac gctcgttgta caggccaaag tttttagagt aggaactggc aacaatcagc
      541 tctttatgca tagccgcgaa agcgcgcagt ccttcagcat cttcttccag accacgggca
      601 aaaccctggt aagcgaagtc aaacagcggt aaccagcctt tctcaactga gagttgtgcc
      661 agtgtctgcc attgttccag cgtagggtcg ataccggttg ggttatggca gcagccatgg
      721 aacagcacta cgtcgccagc ctgagcttcg ttcaggctgt taatcagtgc atcgaagtca
      781 agggtgtggt tttctgcatc ataataagcg tattcacgaa cttccagacc tgcagagtta
      841 aagacgctct tatggttcgg ccagcttggg ttgctcaccc acactcgctt aacgctggta
      901 ttttttgcca ggaaatcggc agctacgcgt agtgcgccag tgccccccgg agtctgtgcc
      961 gtgcgagcac gtttgtcatt gatcagggcg ctacctttac caaacagcag ttcctgagtg
     1021 cagcgaccaa attcagggat gccgtcaatg ccgaggtaat ttttggtggt ttcattttcg
     1081 agcagatact gttcagcctt tttcacgctg gtcaataccg gggttttgcc cgtctcatct
     1141 ttatagacac caatcccgag gttaattttg ccgggacgtt catcggcacg aaacagatcg
     1201 gccaggccca gaatcgggtc ggcaggagcg gcggtaatgt tctcaaacat gacgaggttc
     1261 cattatggtt acagaaggga agtccgctat cagggtaacg ggagatttac aaaattccaa
     1321 ctattactga tgaaaacgca ggctgttttt gcaagacgtg agattgctct ggaaggcata
     1381 aaaaaaacag gaccaaagtc ctgttttttc ggcatttaac aaagaggtgt gctattagaa
     1441 ctggtaaacg atacccacag caacggtgtc gtcagaacct acgcccagtt tgttgtcaga
     1501 atcgatctgg ttgatgatgt agtcaacata ggtggacatg tttttgttga agtagtaggt
     1561 tgcgcccact tcaaagtagt tcaccagatc aacatcaccg ataccttcta cgtctttcgc
     1621 tttagatttg gtgtaagcga tggacggacg cagaccgaaa tcgaactggt attgcgcaac
     1681 taacagaacg tcttgcgttt tgttggcgaa gccgctggtg tttgtaaatt tattagtgat
     1741 cggcgtagcg ttacgggttt caccgtagtt cgctgccagg tagatgttgt tcgcgtcgta
     1801 cttcagacca gtagcccact gttcagcttt tttaccgttg ccaagaggtt gagcttcttg
     1861 caggttggta cggtcagctg caccataagc accaacgata ccaaagcctt cgtattcgta
     1921 gctgatagaa ccgccaacac cgtcgccgtt agaacggcgt gcagtgtcac gctcgttttt
     1981 acccaggtac tgaacagcga agttcaggcc atcaaccaga ccaaagaagt tggagttacg
     2041 ataggtagca acgccgccaa cacgaccaac gaagaagtca tcgctgtatg cagtatcacc
     2101 accaaattct ggcagcatat cggtgtaacc cagtgcatca taaaccacac cgtagttacg
     2161 gccgtaatcg aaagaaccaa cgtcagcgta tttaagaccc gcgaatgcca gacgcgtttt
     2221 gttaccagtt tgagcgtcag cgccttcaga gttgttaccc tggaagttat attcccactg
     2281 accataaccg gtcagatcgg aattgatttg agtttcccct ttaaaaccaa gacgggcata
     2341 ggtcatgtcg ccattgccac cgtaactgtt ttcaccgtta cccttggaaa aataatgcag
     2401 accaacagct ttaccgtaca gatctacttt gttgccatct ttgttataga tttctgcagc
     2461 gtttgcagta cctgctacta acagagcagg gacgatcact gccagaatat tgcgcttcat
     2521 cattatttat taccctcatg gtttttttta tgacacctgc cactgccgtc aataagttct
     2581 gtcaataaaa atttacggaa ctattgatga gagtttggtg tctttatgtg tctgcaggca
     2641 tctttccat
//
LOCUS       DAZGJW010000332         2616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZGJW010000332 DAZGJW010000000
VERSION     DAZGJW010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.335"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(473..1663)
                     /locus_tag="ACHKIK_005247"
                     /pseudo
     CDS             complement(473..1663)
                     /locus_tag="ACHKIK_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131474.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1927..2127)
                     /gene="torI"
                     /locus_tag="ACHKIK_005248"
     CDS             complement(1927..2127)
                     /gene="torI"
                     /locus_tag="ACHKIK_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFO9086192.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2185..2352)
                     /locus_tag="ACHKIK_005249"
     CDS             complement(2185..2352)
                     /locus_tag="ACHKIK_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086193.1"
                     /translation="MHFRVTGELNGEPFNRVIEAEDINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(2388..>2616)
                     /locus_tag="ACHKIK_005250"
     CDS             complement(2388..>2616)
                     /locus_tag="ACHKIK_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086194.1"
                     /translation="CGGNHGKAAMIECKDKIHICLNCVDVLVDIKNEREDKKRSEAIR
                     ALDSWMRDGYSAAQIYDLAISKGEIPGVRIE"
BASE COUNT          661 a          535 c          558 g          862 t
ORIGIN      
        1 gcgtccttta gataaactga aaaatattaa gcatgtctca tgtggttttc tgaatcaaaa
       61 ctcttttcag tctttgattt tttgttaaaa acaggtttat tgcaagggtg ttgttgtgaa
      121 atgtgttttg gtgtcgttgt gattttatgg tgataatgtg aatttttatc ctttcaagat
      181 ggctccctgg acgttaaata cagtggaaat ttatattaag atataaatat aactattgcg
      241 ttatttaaat tattgtctat tacagctcca gttaatagag cctgaagtaa tttcttagta
      301 tttcatcata agcgtttatc tatcgtctct ttctaaatta accggtcgta ttacaaggtg
      361 ctgaatctga ttaaggaatg atacttaggg gattatggct gacttaataa ttagataact
      421 gataaatgac tatcaggggg atatcgttaa gtcagccttt aaaatcaagc gattatatag
      481 ctggtgactc ttttcgaaga gcatcaaggt agtcagcgta ccattgcatc atttctcgtc
      541 ttccttcaag gtaacgagca tggttgtatg taccacgaat gctgtttttg tccgtatgtg
      601 caagttgcat ttcaatccat gcactattga atccttgctc gtgtagaaca gtactcattg
      661 tatgacgaaa tccatgcccg gtagcttttt tgtcatagcc aatcttcttc aaaaccatat
      721 tgacgcttgc atcgctgata ggcctgttaa catcatttct tccggcaaag atgtatctgt
      781 atcttccagt cagttctttg aggatttgca gagactggat tgcctgatct gacagaggga
      841 cgatatgctc cttcctcatt ttcatccggg tcggaggaac ttcccatatc ttttgggtaa
      901 aatcgatctc acaccattcc cccatacgta attctactgt tctcagccct gtgagcatca
      961 acaattttgt agcttcacgg gttacaatac tgccggttta gttttcgagt ttgtataaaa
     1021 actctggtag ttcaggagga agcaggaaag ggtaatgtgt tcgcttatag cttttcattg
     1081 ctataaccag atcagaagct ggattgtatc gtgcccgacc tgtaacaatg gcataccgaa
     1141 acacttcacc acatcgttgg cgaactttat tagctagctc gcaggctcct cggctttcga
     1201 tacgctgaag aactgcgaga agttcaagag gttcgatttc actaatgggt ctgtttccaa
     1261 tgtagggaaa cacattattt ttgaatgctc tgtcgacata atcagcgtag ctggcagacc
     1321 agcctgaaac ttttgcgttg taccactcgg tggcgattgc ggcaaaagtt cttccttcgg
     1381 aaaggttttt ctcaagattt tcagctttct tgacttcacc gggatcttta ccatcggcat
     1441 gtattttctt ggcttcatcc cgacgtttgc gagcttcagc caatgtaata gttggatagg
     1501 tgccaaatga tagtttcttt tccttccccg catagtggta cttcattcgc cagtactttg
     1561 agccgttagg attgacctga aggtagagcc caccaccatc ggcaatctta taaattttat
     1621 ctttgggttt ggctgtttca acttgtcgag ctgtcagtgg catctggggg cctcgcaata
     1681 gattgaccaa gatgtgcccc taaataggcc cccaaatgtg acttgattta cgttcccctg
     1741 aggagaatta aggcgactat ttgagtaaca taagtaaggg tatgtgctga ttataaaggc
     1801 tttttgtggg gtatggtaga tttcaggaga ggtgtgcatg gtgtcccctg cagacatcta
     1861 cttagagtgg caggggattg attagaatgg tattttttag atgtgagaaa tattttaccc
     1921 gctattttac ccattagcgc ggcttaagag cttatttttg aattcacaat ggtcacgata
     1981 taaccatctt gctcgaccgt ggataacttt ggctttaggc aggtcgccgg acttaatccg
     2041 gtcgtagatg aaggttttac caaagccagt atcagccatg atgaatttca aatcaaccag
     2101 tgaatcaggt tgtagttcgt gttgcatgag tgctatctcc gaatagggaa tcgaacctgc
     2161 aaatcaggca ataaaaaacc gccatcaggc ggcttggtgt tctttcagtt cttcaattcg
     2221 aatattggtt acgtctgcat gtgctatctg cgcccatatc atccagtggt catagcagtc
     2281 gttgatgtcc tctgcttcga taactctgtt gaatggttct ccattcaatt cacctgtgac
     2341 tcggaagtgc atttgtgatt tccccaaaag atgcttgagt gcgcttctta ttcgattcgc
     2401 acacctggta tttcgccttt tgaaatggct aagtcataaa tttgcgcagc actataccca
     2461 tctcgcatcc atgaatctaa ggcgcgaata gcctcgctac gctttttatc ttctctttca
     2521 tttttgatat caacgaggac atcaacgcaa ttaaggcata tgtggatttt gtccttacat
     2581 tcgatcatgg cagctttacc atgatttccg ccacac
//
LOCUS       DAZGJW010000333         2600 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZGJW010000333 DAZGJW010000000
VERSION     DAZGJW010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.336"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..762
                     /locus_tag="ACHKIK_005251"
     CDS             <1..762
                     /locus_tag="ACHKIK_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFO9086195.1"
                     /translation="VSKSQLDDIADTPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLE
                     PEEFSNRFIVAPEFNRRTNAGKEEEKAFLMECASTGKTVITAEEGRKIELMYQRVMAL
                     PLGQWLVESAGHAESSIYWEDPETGILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAY
                     YDYRYHVQDAFYSDGYEAQFGVLPTFVFLVASTTVECGRYPVEIFMMGEEAKLAGQQE
                     YHRNLRTLADCLNTDEWPAIKTLSLPRWAKEYAND"
     gene            755..1564
                     /gene="recT"
                     /locus_tag="ACHKIK_005252"
     CDS             755..1564
                     /gene="recT"
                     /locus_tag="ACHKIK_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFO9086196.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            1621..1815
                     /gene="ralR"
                     /locus_tag="ACHKIK_005253"
     CDS             1621..1815
                     /gene="ralR"
                     /locus_tag="ACHKIK_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFO9086197.1"
                     /translation="MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGGVHV"
     gene            1808..1996
                     /locus_tag="ACHKIK_005254"
     CDS             1808..1996
                     /locus_tag="ACHKIK_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO9086198.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLLSS"
     gene            2103..2384
                     /locus_tag="ACHKIK_005255"
     CDS             2103..2384
                     /locus_tag="ACHKIK_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO9086199.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLLEKLKHAAEKIRSQFT"
     gene            2350..>2600
                     /locus_tag="ACHKIK_005256"
     CDS             2350..>2600
                     /locus_tag="ACHKIK_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO9086200.1"
                     /translation="MQRKKYDPNLPKNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERY"
BASE COUNT          840 a          556 c          607 g          597 t
ORIGIN      
        1 gtcagtaagt ctcagctcga tgacattgct gatactccgg cactgtattt gtggcgtaaa
       61 aatgcccccg tggacactac aaagacaaaa acgctcgatt taggaaccgc tttccactgc
      121 cgggtacttg aaccggaaga attcagtaac cgctttatcg tagcacctga atttaaccgc
      181 cgtacaaacg ccggaaaaga agaagagaaa gcgtttctga tggaatgcgc aagcacagga
      241 aaaacggtta tcactgcgga agaaggccgg aaaattgaac tcatgtatca acgcgttatg
      301 gctttgccgc tggggcaatg gcttgttgaa agcgccggac acgctgaatc atcaatttac
      361 tgggaagatc cggaaacagg aattttgtgt cggtgccgtc cggacaaaat tattcctgaa
      421 tttcactgga tcatggacgt gaaaaccaca gcggatattc aacgattcaa aacggcttat
      481 tacgactacc gctatcacgt tcaggatgca ttctacagtg acggttatga agcacagttt
      541 ggcgtgctgc caactttcgt ttttctggtt gccagcacaa ctgttgaatg cggacgttac
      601 ccggttgaga ttttcatgat gggcgaagaa gcaaaactgg caggccagca ggaatatcac
      661 cgcaatctga ggaccctggc tgactgcctg aataccgatg aatggccagc tattaaaacg
      721 ttatcactgc cccgctgggc taaggagtat gcaaatgact aagcaaccac ctatcgcaaa
      781 agccgatctg caaaaaaccc agggaaaccg tgcaccagca gcagtaaaaa ataacgacgt
      841 gatcagcttt attaatcagc catcaatgaa agagcaactg gcagcagctc tcccacgcca
      901 tatgacggct gaacgaatga tacgtatcgc caccacagaa attcgtaagg ttccggcgct
      961 aggaaactgt gacaccatga gttttgtcag tgcgatcgtt cagtgttcac agctcggcct
     1021 tgagccaggt agcgccctcg gccacgcata tttactgcct tttggtaata aaaacgaaaa
     1081 gagcggtaaa aagaacgttc agctaatcat tggttatcgc ggcatgattg atctggctcg
     1141 ccgttctggt caaatcgcca gcctgtcagc ccgtgttgtc cgtgaaggtg acgagtttag
     1201 ttttgaattt ggccttgatg aaaagttaat acaccgcccg ggagaaaacg aagatgcacc
     1261 agtgacccac gtctatgctg tcgcaagact gaaagacgga gggactcagt ttgaagttat
     1321 gacgcgcaaa cagattgaac tggtgcgcag ccagagtaag gctggtaata acgggccatg
     1381 ggtaactcac tgggaagaaa tggcaaaaaa aacggctatt cgtcgcctgt ttaaatacct
     1441 gcctgtctca attgaaatcc agcgtgcagt atcaatggat gaaaaggaac cactgacaat
     1501 cgatccggca gactcctctg tattaaccgg ggaatacagt gtaatcgata attcagaaga
     1561 ataattcagc ctggcggtgt aatgcaccgc caacgtgaga cagtttttat gacaaaaatt
     1621 atgagatatg acgatgttaa accatgtccg ttttgtggtt gtccatctgt tacagtgaaa
     1681 gcaatttcag gatattaccg ggcaaaatgc aacggatgcg aatcccgaac tggctatggt
     1741 ggaagtgaaa aagaagcact cgaaagatgg aataaacgaa ctactggaaa taataatgga
     1801 ggtgttcatg tataaaataa ctgccactat tgaaaaagaa ggtggcactc ctactaactg
     1861 gacaagatat tcaaaatcta aattaacgaa atcagaatgc gaaaaaatgc tctcagggaa
     1921 aaaagaagca ggcgtgtcca gagagcagaa agtaaagctg ataaatttta attgcgagaa
     1981 acttctgtcc tcgtgagttg cattatatac aaattagaac ttcatagctg attattaaaa
     2041 atcaaccaca cccgccagta ttctgtatat ttactggcgg tcatatcgta agaggtatgg
     2101 caatgaatct tgtgacactc aaaacgtggg gaaaactcag atatccggat aacccaccat
     2161 caatatcaac gctgagacga tgggcaagga atggaaacat ttatcctgca cctgaactac
     2221 acgggaggag ttacagggtg gttccggaag ctttctatat caacccaaat aaggttgata
     2281 ccgatataac acaccatcag cctaatgggc gacaagggag agacagtccg ttactggaga
     2341 agttaaaaca tgcagcggaa aaaatacgat cccaatttac ctaaaaactt aacatatcga
     2401 aggagggaca aagcatatta ctggcgcaac cctctgacga aagaagaatt tacactaggt
     2461 aaaatttcaa gaagagatgc agtagcgcag gcaattgaag caaatcatta tatatacaaa
     2521 aactactctc ctgctgcctt aattgaaaaa ctaaaagggt tcgactcatt tactatggca
     2581 gactggattg aacgttacaa
//
LOCUS       DAZGJW010000334         2597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZGJW010000334 DAZGJW010000000
VERSION     DAZGJW010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.337"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..238
                     /locus_tag="ACHKIK_005257"
     CDS             <1..238
                     /locus_tag="ACHKIK_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HFO9086201.1"
                     /translation="DKNEEVRIEAIIGLSYRKDKRVLSVLCDELKKNTVYDDIIEAAG
                     ELGDKTLLPVLDTMLYKFDDNEIITSAIDKLKRS"
     gene            266..1224
                     /locus_tag="ACHKIK_005258"
                     /pseudo
     CDS             266..1224
                     /locus_tag="ACHKIK_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1236..1697
                     /gene="yibG"
                     /locus_tag="ACHKIK_005259"
     CDS             1236..1697
                     /gene="yibG"
                     /locus_tag="ACHKIK_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO9086202.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSA
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            2332..>2597
                     /locus_tag="ACHKIK_005260"
     CDS             2332..>2597
                     /locus_tag="ACHKIK_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO9086203.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGL"
BASE COUNT          818 a          465 c          599 g          715 t
ORIGIN      
        1 ggataaaaat gaagaagtcc gtattgaagc aataatcgga ctttcctaca gaaaagataa
       61 aagggtttta tctgttttat gcgatgagtt aaaaaaaaat actgtttatg atgatatcat
      121 tgaagctgcg ggtgaattag gtgataaaac gctacttcct gttttagata ctatgttgta
      181 caagtttgat gacaatgaaa ttataacttc cgctattgat aagctgaagc gttcatagcg
      241 tgagttgcct atgcacagtg ggggattccc gccggcacgg tgcagatgct cgaccggctg
      301 gaaagtgaaa tcctggctga ccgggtgagt gaggaaagcc gccgctggct ggcatcgtgc
      361 ggcctgaccg tggagcagat gaaaaaccag atggacccgg tgtacacgcc ggcgcgaaaa
      421 atccacctgt accactgcga ccatcgcggc ctgccgctgg cgctcatcag cacggaaggg
      481 gcaacagcgt ggtgcgcaga atatgatgaa tggggcaacc tgctgagtga tgagaacccg
      541 catcatctgc agcagctcat tcgcctgccg ggtcagcagt atgatgagga gtccggcctg
      601 tattacaacc gccaccgcta ttatgacccg ctgcaggggc ggtatatcac tcaggatccg
      661 attgggctga aggggggatg gaatttttat cagtatccgt tgaatccggt cataaatgta
      721 gatccgcaag gtttggttga tataaattta taccccgaaa gtgatcttat ccattctgta
      781 gctgatgaga ttaatatccc aggcgttttc acaatcgggg ggcatggtac ccccacatct
      841 attgaatccg caacgcgcag tatcatgaca gctaaagatc tagcatatct aattaaattt
      901 gatgggaatt ataaagatgg gatgacagtt tggttatttt cttgtaatac aggtaaagga
      961 caaaattcat ttgctagcca attagctaaa gagttacata caaatgtaat aggacctgac
     1021 acgctatgga cgtggtgggg gcgaggaact aatggtaagt taaaaatgga tacagtgcta
     1081 acagcaccaa cgaaccttaa ttcaaataag gatctaatgg ctataacaac aaaagacctt
     1141 ggtaattgga taacatatgg gccatctggg caccccattt ctaatatgca aggtacgcca
     1201 gaaaaaccca gtgatataag ataggttgta gatgtatgaa agcatgcttg ttactatttt
     1261 tttatttttc tcttattggt caattgcatg gtgctgatgt gaaaataaaa gaaaacgaaa
     1321 gtgtgatggg atctacagca atgacctatg atttaagtga agaaaagctg atgaaactga
     1381 agtataaatc acaacatggt gattcagagg catcatttcg actatatcaa tattactgct
     1441 ttactaaaaa taatattgat aaacaattgc gattcttgga aagatcagca tctcagggga
     1501 atgttacagc gcagtttaat tatggggtct ttttatcagc tacaaatcca acattatcag
     1561 aatattataa tttgaataga gctatttatt ggatggaatt cgctgtaaat aacggtaata
     1621 ttgatgcaaa gagcaaactt caagagctca aaaagctaaa gcgaatggat agaaggaaga
     1681 ataaggagaa tccgtaaaag caatagttca tcagactgcc gcagcagtag tatgatgatg
     1741 aatcagggct gtactataac tattatcggt aacattaacc gcagcagggg cggtataaca
     1801 ctcaggctcc gattgggctg gaaggtaggt ggaattttta tcagtatccg ttgaacccga
     1861 tttctggaat cgatcccctt gggattagcc acctgtttat acagtataac tttaggtatg
     1921 ctttcgtgtg tttcagatat accatgtgat gataattttt atgatgtgct tagtattcca
     1981 gtcgcttctg gtaataatat gcaatgtaaa aataatcccc gttgtacaca tttataaata
     2041 gatggccaat acctcaaggc ctatggagtt agaatgttaa tgacccggga gcaagcaata
     2101 gaaaacctga cggcatacga ttagtaccgt cagttaatac aggaacttat aatcgcaatg
     2161 gattttcaat acatagttgt ttaaacgctt ttggtccgtc tttaggacct cgattctgtt
     2221 ccgagggttg tataactggt ttatctaatg acatgcaaaa attaaatgag ttaatatttt
     2281 cagagccaga tagcacatta acagttacgg actaaaaaat atggcaaggt tattaacaat
     2341 tgtatttttt tctctatttc tgcgagtgca gttactgcaa gacaatttca gagagagtta
     2401 gatttggcag acaaagcaat attatgggca ctaatatcag ccagtactaa agagggacga
     2461 aaagcgtgtt cactaagcta ttttgcctgt aaggccgcag aggctgaact cggactggca
     2521 tatatggctg cgaatgataa caaggaattt ctcacatcct tatctaatat aatgaggtac
     2581 aaaatagatg ctggact
//
LOCUS       DAZGJW010000335         2586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZGJW010000335 DAZGJW010000000
VERSION     DAZGJW010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.338"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..579
                     /locus_tag="ACHKIK_005261"
     CDS             <1..579
                     /locus_tag="ACHKIK_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086204.1"
                     /translation="ALRCAAYIDFSEKDMLKSIIPAWIQAIGSILAIIIAGMISANQI
                     KHEKQLEKRKVAESDLAKMHIVRALIIRSLTLMNEVRQAIERGEESDFQQVSPWTMRN
                     TKRAIDNLNLFEIPEGMLALDLLAIGPSLEEIATLWERCMEESLHTENNKPTIDSYNE
                     LDQGSQEVLKICEAAFKLATQEVNRLAAIVKE"
     gene            1036..1461
                     /locus_tag="ACHKIK_005262"
     CDS             1036..1461
                     /locus_tag="ACHKIK_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9086205.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIATSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            1458..1808
                     /gene="tnpB"
                     /locus_tag="ACHKIK_005263"
     CDS             1458..1808
                     /gene="tnpB"
                     /locus_tag="ACHKIK_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9086206.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRRNSLTML"
     gene            1839..>2586
                     /locus_tag="ACHKIK_005264"
     CDS             1839..>2586
                     /locus_tag="ACHKIK_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HFO9086207.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMT"
BASE COUNT          706 a          580 c          640 g          660 t
ORIGIN      
        1 gcgttaagat gtgccgccta tattgatttt tctgaaaaag acatgctcaa gtcaatcatt
       61 cctgcttgga ttcaagccat tggtagcatt ttagcaatta ttattgccgg tatgatctcg
      121 gccaatcaaa ttaaacatga gaaacagctt gaaaagagaa aggtagcaga gtcagatttg
      181 gctaaaatgc atatagtaag agcgctgata atacgttcgt taactttaat gaatgaggtt
      241 cgtcaggcaa ttgagcgtgg cgaagaaagc gactttcagc aagtgtctcc ttggactatg
      301 cgtaatacaa aacgtgcaat tgataattta aatttatttg aaataccaga ggggatgtta
      361 gctctcgatc tgttggctat aggtccatct ctcgaggaaa tagcaacctt atgggagcgg
      421 tgtatggagg agtcactaca tactgaaaat aacaagccaa ctattgattc ttacaatgag
      481 cttgatcaag gctctcaaga ggttttgaaa atatgtgaag ccgcttttaa attggctaca
      541 caagaggtta atcggcttgc agctatcgtt aaagagtaac tttcacatag gaatttattt
      601 tatatgtgct cttagagtac attactttta gtaatttgta ctctttggtt gtatatgccg
      661 acaacgatat aggttttaaa gtattactgt gaatttatgg ggtaacatca ttataagccg
      721 attaatcctc ctagattact tacctatcct tgaagaactg tcctacagcc cgcttcgtgt
      781 cataagcggg ctgtaggcat agaataacta atcaatcatt agagtgagac tattttcagc
      841 tttggtgagg tgggatgatg attgctttag agtttaatag gacactccgg tcgctccctt
      901 cgtatctgga tgtaaagccc cctatttttt ctccaaaacc ttaccttatc gtaagcgtaa
      961 actgaccgcc gtatgtagcc atcagacgag aattggtaac ttagacgccc atctgatata
     1021 gacggacatc taagtatgga attacaggac tggcgaaaag aacctcgtaa aaactattcg
     1081 aatgaattca aacttcgtat ggtggaactg gcatcacaac ctggagcttg tgttgcacag
     1141 attgcacgtg aaaatggcgt caatgataat gttattttca aatggctcag gctctggcag
     1201 aacgaagggc gtgtttcgcg gcgtcttccg gtaacgacct cttctgacac tggcgttgaa
     1261 ttattacctg tagaaataac gccggatgag cagaaagaac ctgtggcggc cattgcgacg
     1321 tctttatcca cttccactca gaccagagtc agtgccagtt cctgcaaggt ggaattccgt
     1381 cacggtaaca tgacgctgga aaatccatcg ccagagctgc tcacggtgtt gatccgtgaa
     1441 ctgaccggga ggggaagatg atctcactcc catcaggtac ccgtatctgg ctcgttgccg
     1501 gcgttaccga tatgcgtaaa tccttcaacg gactgggaga acaggtacaa catgtgctga
     1561 atgataatcc cttctccggt cacctgttta tcttccgtgg ccgacggggt gacaccgtta
     1621 aaattctttg ggctgatgct gatggtctgt gcctgttcac caaacgcctg gaggaaggcc
     1681 agtttatctg gcctgcggta cgtgacggca aggtatccat tacccgctcg caactggcaa
     1741 tgctcctcga taagctggac tggcgtcagc caaaaacatc ccgccgtaac tcactgacaa
     1801 tgttgtaaaa aactcctgac cgcattataa aaacggtcat gagtcagaaa tacctcattc
     1861 gcatcgcaga gctggaaagg ttgctctctg agcaggctga agccctccgt cagaaagacc
     1921 agcaactgag tctggttgaa gagacggaag ccttcctgcg ctctgcactg acacgtgccg
     1981 aagaaaagat cgaagaagat gaacgggaaa tagaacatct gcgggctcag atagaaaaac
     2041 tgcgccggat gctgttcggt acccgttctg aaaaactgcg tcgtgaagtt gaactggctg
     2101 aggccctgct gaaacaacgt gaacaggaca gcgatcgtta cagtgggcgg gaagacgatc
     2161 ctcaggttcc ccgccagttg cgacagtcgc gccatcgtcg tccgttaccg gcacaccttc
     2221 cccgtgaaat acaccgcctg gagccagaag aaagctgttg cccggagtgt ggcggtgagc
     2281 tggattatct gggggaagtc agcgctgaac agctggaact ggtgagcagt gccctgaaag
     2341 tgatccgcac agaacgggta aaaaaagcct gtacaaaatg tgactgtatt gttgaagcac
     2401 cggcgccgtc ccgcccgata gagcgtggta tcgcgggccc cggattactt gcccgcgtgt
     2461 taacgggaaa atactgcgaa catctgccac tgtatcgtca gagtgaaatc tttgcccgcc
     2521 agggcatcga actgagccgg gcattactct ccaactgggt tgacgcatgt tgtcagttaa
     2581 tgactc
//
LOCUS       DAZGJW010000336         2579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZGJW010000336 DAZGJW010000000
VERSION     DAZGJW010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.339"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..225)
                     /locus_tag="ACHKIK_005265"
     CDS             complement(<1..225)
                     /locus_tag="ACHKIK_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO9086208.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLLEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQL"
     gene            complement(258..557)
                     /locus_tag="ACHKIK_005266"
     CDS             complement(258..557)
                     /locus_tag="ACHKIK_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO9086209.1"
                     /translation="MEQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGLTNKKRPVCKTERSEQIQMEF"
     gene            complement(696..929)
                     /locus_tag="ACHKIK_005267"
     CDS             complement(696..929)
                     /locus_tag="ACHKIK_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9086210.1"
                     /translation="MSNELLRWRKEASSEEWKRLAALANTSVGYLDQIVYGFRRASPD
                     KANAIEEATRNFTGYKPVKKENLVFVSRRASAA"
     gene            1043..1747
                     /locus_tag="ACHKIK_005268"
     CDS             1043..1747
                     /locus_tag="ACHKIK_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9086211.1"
                     /translation="MENKDIRKSNLAFLLDEHKKIAGNTNASFADKLGVSPSQLTQVS
                     GEKSTRNIGDKLARKFEAALGLPNGWLDLVHDVTPIASCSDSLTFVGQVRKGLVRVVG
                     EAILGVDGAIEMTEERDGWLNIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKVF
                     PGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILKQ
                     SRHLDDIEAREWLKSS"
     gene            2016..2378
                     /locus_tag="ACHKIK_005269"
     CDS             2016..2378
                     /locus_tag="ACHKIK_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086212.1"
                     /translation="MTQNVLPINKSLHDRAVDEFNRLHGTMIGEISAMLKTAKVAPLV
                     DLRKKDPTFSNVVAELRTFRDVCNALLPYFRVDKTSEIAVIDKLLILANDLAQAIDAD
                     DPDALCAAIAALDVEPYI"
     gene            2394..2522
                     /locus_tag="ACHKIK_005270"
     CDS             2394..2522
                     /locus_tag="ACHKIK_005270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086213.1"
                     /translation="MTKAFDYTAVSKLLAEMRGCVERVQNLRRDFEAHINHSQKAA"
BASE COUNT          732 a          526 c          588 g          733 t
ORIGIN      
        1 aagttgagaa tcggtgattc tgtccattgg tttattccac ccataggttt tacgcagaat
       61 ggcaagcagc actttaaact gtcgcttggt cagatctgcg cctgaatagg cctcaagcag
      121 catatttgat agtctggcgt aaccatcatc gagatctgcc acattacgct cctgttcggc
      181 aaagttacct ctgccgaagt tgagtatttt tgctgtattt gtcataatga ctcctgtgga
      241 ttgatccagt aattccctca gaattccatc tggatttgtt cagaacgctc ggtcttgcac
      301 accgggcgtt ttttattggt gagtccatca agcgcatact taaaagctct gctaatcgga
      361 ctgatgtctg atgccattcc gaaagcacac aagaccgaag caataaatct ccagtccgtt
      421 ctgcttatct tcgattcatg acagccaatc atctttgcca gaccgcgctg ggtaagcgtt
      481 gacaggttga taagtaaatc tgtttctgcg cgatcaacat cacgctgtga tagtttgctg
      541 taacttgttt gttccatttc ttaagatttc caatagtgaa tagctagttg aaaggtatgc
      601 gtggaaacgc atatggcctt agttggtcag atatcttgga actcgctttt cagcgacgta
      661 ggacgaatgt ccgttgttac aaagagcggg gttacttatg ctgctgatgc tctacgcgat
      721 acgaacacca gattttcctt tttcacaggt ttataacccg tgaaattacg agtggcttct
      781 tcgattgcat tcgctttatc tggggaagct cttcgaaacc catatacaat ctgatcaaga
      841 tagccaactg aagtattcgc taatgcggcg agtcgcttcc attcctcact agaagcctct
      901 tttcgccagc gtagtagttc attactcatt agtgcctccg tttatcacac agagcgactt
      961 taccattttg ataaataaaa cgcaatgaag atttatcaaa atgcatattt atccatttgc
     1021 taaatagagg gagaatcgtg ggatggaaaa caaagatatt cgcaaatcga atctggcgtt
     1081 tttgctagat gagcataaaa aaatcgcggg taacactaat gcaagctttg ccgataagct
     1141 tggagttagc ccttctcaac tcacgcaagt ctccggtgaa aaaagcactc gaaacatagg
     1201 ggataaacta gcaagaaaat tcgaagccgc acttgggtta cctaatgggt ggcttgattt
     1261 ggtacatgat gtaacaccaa ttgcatcatg ctcagattct ttaacttttg tcggtcaggt
     1321 aagaaaaggg ttagtgcgcg tggttggtga ggcaattctt ggtgttgatg gtgccatcga
     1381 gatgaccgaa gagcgcgatg ggtggctcaa tatttatagc gatgatccag atgcctttgg
     1441 tcttcgtgtg aaaggagaca gcatgtggcc cagaataaaa tcaggagaat atgtactcat
     1501 tgagcctaac accaaagtat tcccggggga tgaggtgttt gtcagaactg ttgaaggaca
     1561 caacatgatc aaagttcttg gctatgacag agatggagaa taccaattta caagcatcaa
     1621 ccaggaccac aggccaataa cgttgcctta tcatcaagta gcaaaggtgg agtatgtggc
     1681 tggtattctg aagcaatctc gccatctgga tgacatcgag gcaagggagt ggctgaaaag
     1741 ttcgtgactt catcgtcaca tagctggtaa ccagtggcct gaagagacgt ttgggtgatg
     1801 tgatagagat cattattcaa ttcgattgag aaaaatcatc ataaggttta tatccattta
     1861 ttagatttta tgtgtataaa tagttaaaaa tgcacgatgt agagatattt gtgcttaaca
     1921 tcaattaatc attgctaaat cgtgcttatt gttcagaaat cgcccaagtc atattgaaaa
     1981 tctgttggcg aacacctata taaggagtat agttagtgac ccaaaacgta ttgccaatca
     2041 acaagagctt gcatgaccga gctgttgatg aatttaatcg tctgcatgga acaatgattg
     2101 gcgagattag tgcaatgctg aaaacagcta aagtagcgcc attggtggat cttcgtaaga
     2161 aagatcctac cttttcgaac gttgtcgcag aattacggac attcagagac gtatgtaatg
     2221 ctctgctccc atactttcgt gtagataaga ccagcgaaat cgctgtcatc gataagttac
     2281 ttatactggc taatgacctg gctcaagcca tcgatgctga cgatcctgac gcattgtgcg
     2341 cagcaatagc agcccttgat gttgagcctt acatttaaac gaggggaaaa aaaatgacta
     2401 aagcgtttga ctatactgcc gttagtaagc ttttggctga aatgcgcgga tgtgtagagc
     2461 gcgttcaaaa tctacgccgc gactttgaag ctcacatcaa tcattcacaa aaagcggctt
     2521 gatacttaag ttagatgaca gaacccggcc tcagcgccgg gttttctttg cctcacgat
//
LOCUS       DAZGJW010000337         2576 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZGJW010000337 DAZGJW010000000
VERSION     DAZGJW010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.340"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(201..1559)
                     /locus_tag="ACHKIK_005271"
     CDS             complement(201..1559)
                     /locus_tag="ACHKIK_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFO9086214.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            1841..1972
                     /locus_tag="ACHKIK_005272"
                     /pseudo
     CDS             1841..1972
                     /locus_tag="ACHKIK_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2292..>2576)
                     /locus_tag="ACHKIK_005273"
     CDS             complement(2292..>2576)
                     /locus_tag="ACHKIK_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFO9086215.1"
                     /translation="LLTEPWGRKMHSDYYNMVFGEKLANILYEANSQFFYERNVIEEA
                     VNALFCEREIINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          637 a          647 c          594 g          698 t
ORIGIN      
        1 tttgttcgga ctgcttatgg cctttttcca ctggtgtagc aggaaagtta accggctgcc
       61 accatgagat gacgtgtaaa ccgtgcaggc atgttttcac gtggtgacgc tttcattggg
      121 ggtctacgcc ggaaccccaa tattgttcac atacatgaat ttgaaaaact aagcgatact
      181 gctgtccgta ccttataaac ttatttcaac gcttccggca gcgtcacaat ccacagttca
      241 ctgtcagact ccggtttctc cagtggcttc actctgaggg tcgtttcaac ctgcttgtcg
      301 tcaagcgtca gctttccttc cgcgttcacc cgataatcct tgaatgtctt gccctcgacc
      361 ggatgttgca tcgccacctt cacgccaaag tcgttatcgt tcgccaccgc cagcgttttg
      421 ctgtcgatca gcgccaaccc ttcagccttc tcctgctgcc agcccaagga gcgcagatca
      481 acaacctgcg ttttctgcgc aagggtaatg ccgcgctgtg acagggtttt ctcatcatca
      541 aactccggat actcacccgg cttgtcgaag cctgacaggt cgctcgcctt attgagatcg
      601 acctcgtaaa tcaggttacg cattctgttg tttttatctc ttccctgttc gatcagcagg
      661 atgtgctggt tatcgagcgc cacgatgtct ccgattttag cgtcgctgtt tttgctgtag
      721 gccgcgctgt cgataggata accgtacatc gcggtttttc cgcttgccgg atcgaagctc
      781 accagacgcg taaacagcgc ctttttcttg ctcttagcgt cgatatccag cgtactttgc
      841 acggcgacaa taatacgccc gtccggcata cgggtgaggc cttcaaagcc gcggttcgcc
      901 tgacgccatt tgaggatatt tggcaaaccg cccgcaatcg ctttttcccc ttccgccgcc
      961 tgcggaccgt ggattgccag gattttccct ttactgtcga tgttaatcag gaacgggcca
     1021 tactcatcgc acagccagta acctccttta ccatccggag tgatcccttc cgtatccagc
     1081 ccgcgattat cccccttcag tctatgtaac gtgtcgctga aggccacttc attggtggag
     1141 ccaatcacgt cgcttgccag cggcagaccg ttgatcgcgc ctttatcgtc atgcagagga
     1201 cggggatcga tggcctccgc tttgccgttt tgtacacgaa tcgtcatcag cagcggcgcg
     1261 aaatctggcg taacgaaaat tttagtttcg tttttcccct ctttcggtga atccgcgttc
     1321 ggaccgcgat cggtaatggt tgcgaatgtc agggcatcgc cctgtttacc ggtgaacagc
     1381 aggccagaac ctatcccaac cggaaggccg ttcgggaatg cactggcaaa tgcgccggca
     1441 tagttcacgt gcgtgccttc tggaaaactg acgacatagc gctcagccgt aggttgtgcc
     1501 gccagggcag agaaagagag cgtacaaccg attagcacgg gaataatttt tcttttcata
     1561 ttgttagcat ccataaagtg aagatcacac tacaccataa agaagaaacg agtcaggaaa
     1621 atgacatcga atggcaacat gcgcgggcct gaggccaaag aaatataaaa cactgttgtt
     1681 atttgtaaca acggaaacat ttacggaaat tatttgtctt tcttcgtgat caaaaatgct
     1741 gtgtcttgtt ggattggagt gatgagggct gcgtttagga aacgccctat attgtacggt
     1801 tgcattatcg cggtaggggt aatgcacgtg tcagtatgat gagctgacca cactcacgtg
     1861 ggtggactgg tataacaatc gacgattgct ggaaaggcag ggccatattc caccggcaga
     1921 agcagaaaaa gcttattatg cttccatcgg aaacgatgat ctggcagcct gagttcacag
     1981 ataaaacact ctccaggaaa cccggggcgg ttcaattgct ggataagatc ataacgatac
     2041 accgggttaa acaggtggtc actgcttcac tgaagctgcg tgattatgat ggtcaggtag
     2101 cagaagctat gcccatgata ccaaaatgac gaaagccgaa atgtctgaaa gcgtgtgtat
     2161 tacctgaaaa tatgaccggc tccggggtac gtaaccgatt caacaaagcc aatcgatatt
     2221 acaaaacgag agggactata tatttcgttt tcagataaga ctccttgaga gattatcttt
     2281 tatgtaaaat ctcaaatatc acctgatgta atgtcaatga taatattaag tgcgctctga
     2341 acaacatctt tcttggtgtg attaacatct gacaaaaaga acatcagctt ctttattatg
     2401 ttcttgttgt ttattatttc acgctcacag aataaagcgt tgactgcctc ttctatgaca
     2461 tttctctcgt aaaaaaattg agagttagct tcatataaaa tattggccaa tttttcacca
     2521 aaaaccatat tataataatc actgtgcatc tttctccccc agggttcagt caataa
//
LOCUS       DAZGJW010000338         2571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZGJW010000338 DAZGJW010000000
VERSION     DAZGJW010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.341"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(104..904)
                     /locus_tag="ACHKIK_005274"
     CDS             complement(104..904)
                     /locus_tag="ACHKIK_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086216.1"
                     /translation="MEMVVSDGEERFTELDFYYGSKALEGTSEIVTVLTNTILNREVV
                     TKIPSIEGITASFRTSYEGSFRQRFMLKITGEEQRRNYGAIGEGAFLDVMSYYLTRPL
                     LTPYNFTYKRAARIVKELESDYSSLMKRLYGPLLKLHKPIEEQNYTVELKRRRTNLAR
                     YDHTTLENLIVERADDERIIIEASITRFNRLTGTGRLILEEDSESVSFEPSYLWRDFP
                     ADQKRWLSRNLDVNTDNGDFTRVRLEVTSIRNFLGVIKKYSLHRVITD"
     gene            1703..2176
                     /locus_tag="ACHKIK_005275"
     CDS             1703..2176
                     /locus_tag="ACHKIK_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086217.1"
                     /translation="MDIDTLGGQELRDKIFAGLKIYEGKPFIERFGLFMGKAQLLEFG
                     LKKILVSFPGYNLDEEKLERLTLGQTRVELKKLGLRTDYNAYLKSFKDQRNTMAHEFL
                     ANFAVTQQLLDGAALIRTFERELDHACYAVEQLIILFDFINGAGDVTAWLEPTAP"
BASE COUNT          736 a          554 c          504 g          777 t
ORIGIN      
        1 ccattcagac ttatcaattg gaaaaccatt taacggatcg aaattgaaga aataaatcaa
       61 ccatgtaact gatataacta aaaaggcaac atactttatc atatcaatcc gttataactc
      121 ggtgaagact atatttttta atgacaccaa gaaagtttct gattgatgtc acctctagcc
      181 gtacacgagt aaaatcacca ttatctgtgt tgacgtctaa gtttctagat agccagcgtt
      241 tttgatctgc tggaaaatct ctccaaagat atgaaggttc aaaacttaca gactcagaat
      301 cttcctcaag aatgagcctt cccgtccccg ttagccgatt aaatcttgta atagaagctt
      361 caataatgat cctttcatca tcggcacgtt caacaattag attttcaagg gtcgtgtgat
      421 cataccgagc aagatttgtc cttctccgtt tcagctcaac agtataattt tgttcttcta
      481 tgggcttatg aagcttcaat agtgggccat aaagcctctt cattaatgaa ctataatctg
      541 actcaagttc tttaacaatt ctagcagccc ttttatatgt gaaattataa ggtgttaata
      601 atggtcttgt aagataataa gacataacat ctagaaatgc cccctctccg attgcgccat
      661 agtttctacg ttgttcctca cctgtgatct tgagcatgaa acgttgtcta aacgaccctt
      721 cataactggt acgaaaagat gcggttatgc cttcgattga tgggatttta gtaactactt
      781 cgcgatttag aatcgtatta gtgaggactg taactatctc tgacgtgcct tcaagtgctt
      841 ttgacccata gtaaaaatca agctctgtga atctttcttc tccatcactt actaccattt
      901 ccatgggaac atgaatttcc attctctaat tccaaataaa aaccatgggt ttaatatgtc
      961 ctaattgatt tggttttggc aactattttt gattgttttt caagaataat tcgaaagcta
     1021 ttaattaatg gctggaacta ataggttcca gttttcaact aatccagtga cctgctcccc
     1081 gctgatcaac tcagactgtt gttagcaact tacgctcctg gtacggagcg ggcattgatt
     1141 acccgctccg ttcacgttcc gcctggaagt tctgcaattt atgtctatcg gctattgaaa
     1201 accttccggt ccttcccaat atcgaaactg gcacctttgc cagataacaa agagatacgg
     1261 gaacatgatg tacgtctaca gaagacctcc aaatcagctc ctttccattt ctcgtttgaa
     1321 gcagtatttg ttgcatgagg tggaatcaaa tcaacacatc atgagacagt gggaatctgc
     1381 cagaacggca cttcgaaaca tccgtaacaa agggcatgat ccagacaccg cctcctgggt
     1441 ttcacttaaa aatgatcccc gagtcttcac gaatggcgca gatgaaaagc atgggttcat
     1501 ttttgttgac gacgacgatc aatcagtcct tgttgtggcc tcctgggtga tagatgagta
     1561 cggttccccc ctcactacat attaatccct gtacctggac agaagagttt tctgtccaca
     1621 actaccaata gctttacata atgtgtgcat ctaacttcaa taagttactt cattaccaat
     1681 aatgtctaaa aaggaaacgc gcatggatat tgatacgctg ggtggccagg aacttcggga
     1741 taagattttt gctgggctca aaatatacga gggtaaaccc ttcatcgaac ggttcggtct
     1801 gtttatgggc aaggcacagt tgctagagtt tggcctaaaa aaaattcttg tttcttttcc
     1861 gggatacaac cttgatgaag aaaaattaga gaggctgaca ttaggccaga cccgcgttga
     1921 gcttaaaaag ctggggctgc gcacggacta taatgcttac ctgaagtcat tcaaagatca
     1981 gcgcaatacg atggctcacg agttccttgc taattttgcg gtcacacagc agcttttaga
     2041 cggagctgca cttattcgca catttgagcg tgagttagac catgcctgtt acgcggtgga
     2101 gcagttgatc atccttttcg acttcatcaa cggtgccggt gacgtaaccg cctggcttga
     2161 gcctactgcc ccctgatact tcgtatgctt ttatctctag gctggacaga atagtaattc
     2221 tgtccaggta tgcagaatgg ctgtctatat atcttcatta gttcacagta ggttaccggc
     2281 ggctttgagc gaacagcgga acttcgccct ttaataaatg ataataaatt ttcattatca
     2341 ctttagttga aaatggtgtc gaagatgaag agaaatgtca tattagccta cggagtactt
     2401 gtgaaataaa ttcatagcat tccctaacat ctaaacttag ggctcactga acgatggtga
     2461 accctatgcc attgggttaa taactattga taattttgtg aagtgcaggg ttaattgtcg
     2521 atgaaatatc aaaatcggaa tttctaatcg acccagtgct ccatccctct t
//
LOCUS       DAZGJW010000339         2564 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZGJW010000339 DAZGJW010000000
VERSION     DAZGJW010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.342"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            287..1720
                     /locus_tag="ACHKIK_005276"
     CDS             287..1720
                     /locus_tag="ACHKIK_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO9086218.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     FSSACQNQIDVCRPGNLRGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALAEEYA
                     KLVENYSTEKEIWEQKQKALESKLHKEIRAGKDCKATESELKRHLNKCPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGIIFDGRAMSKLGILNSLWDGGSLFIDRKSS
                     PGIILKDPRLTVSVMIQPDVYQKGFCTRKKELVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEQRINELIDENLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIHHSSGEEGDISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNPALTVPVRLIPEELELFNWIKSYCIEKGIPCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
BASE COUNT          756 a          435 c          577 g          796 t
ORIGIN      
        1 tacgtggggc cgttaatatt tacactatgg ttctggagaa agggaaaaga ccgggggaaa
       61 ggtattagtg aaataatttc aggaggtatt aagaatgaga aatatgaatg atgtttcgtg
      121 gtaaataata cagtctgcac tgaattaacc ttatcggttt attaaacatc agatctacaa
      181 atgctctgta tttctcagag catcaggtct gaatagcagc tattttcgct attgtagcgg
      241 gatttgctgc tattttacta acccattact gaaaagagag gcttttatgt gcttacttgc
      301 accagaaaat ccttatccga tatatgcact accaccgctg gtgagaaatg caataattga
      361 aactcaaaaa aatacacagg cacctttggc tatggtggcg acatccgcat taacagcgtt
      421 ctcaagtgcc tgtcagaatc agattgacgt gtgcagacct ggaaatttac gtgggcctgt
      481 taatctttac tctctgattc tggctgattc cggtgaaagg aaaacgaccg tggataaagt
      541 gtttatgaaa gcattttatc tcagggatga agccctggcg gaagaatacg cgaaactggt
      601 tgagaattac agtacagaaa aggaaatatg ggagcaaaag caaaaagcac tggagtcaaa
      661 attgcataaa gaaattcgcg ccggtaaaga ttgtaaggca acagagtcag agcttaaaag
      721 acatctgaat aaatgtcctg ttccgccgca gatacgccgg acaattttca atgagacaac
      781 gatagaggga atgttaaaat attattccga cagcaatcgc tcttttgctc ttgtatccag
      841 tgaaggagga ataatttttg atggccgggc aatgagtaaa cttggaatac ttaactcttt
      901 gtgggatggt ggttctcttt tcatcgacag gaaatcatct cccggaatta ttttgaagga
      961 tccaagactg acggtatcgg tgatgattca gcctgatgtt taccagaaag gtttttgtac
     1021 gcgaaaaaaa gaacttgtga aaacgtcagg acatcatgca aggtttttga tgtgtcaacc
     1081 aacatcaacg caggggacaa ggataataac cggagataat tattcatcac agtatcagga
     1141 tttgtttgaa cagaggatca atgagcttat tgatgaaaat ctggcgatga gtggtgaacg
     1201 acgttgcctt cacttttctc ctcaggctgc cagaatctgg acggattatt acaacgatgt
     1261 ggaatcgaag ctggggggat tggggccttt aagacattgc cgggaatatg ctgctaaaaa
     1321 tgcagagtat atggcaagac tggcaggact tattcaccat tcgagcggtg aagaggggga
     1381 catttcccct tacactgcag aaatggcgag agaattagca atatggtacg gtaatgagta
     1441 tgtgcggttg tctaatccgt taacttttga caaccctgcc ctgaccgtac ctgtgcgact
     1501 cattccagag gagcttgaac ttttcaactg gataaaaagc tattgcattg agaaggggat
     1561 cccctgtatg aaaaaaaatg atattttaca gcgtggtccg aaccgtttcc ggaaaaagga
     1621 taaaatcaac tggttactgg atttactgta tgaacaaaac agagttgtac cggttattga
     1681 gggaaaaacg ttgtgtgtcg cacctaactt tgacctctga acgacttaat cgttgagtaa
     1741 catatagtct cctctgctac ttgcggagga gactaatgcc tttaccacgt cagcgttgtt
     1801 gtattagaaa actctggtac aggtcagtca gtaaatataa ctggtgtaac cactatttac
     1861 tattggatag agtgttgctc aggatattgt cggctttagt ctgaggtgag gtgggacctt
     1921 cattgttttc gtgttcaata aataatttag gttaattagt attattaatg ggagttatgt
     1981 tatctgttat gttatcttat ctctctcttt ctatcgtaat atcactcctc tttatgctaa
     2041 gtattctgta tgtgcgtaat gcatatatct ccatacacta cagtgtggtg gtgtatggag
     2101 tttagttatg tgtaaattat gttactacac ttatagcgta atattgagaa agttaatcca
     2161 ctgaatgtat gtctggtttg tatttaacac tagttacgta ccagaacact attagagtat
     2221 attgtcttta gttcgtacgt tatagctccg ttacagttta tgggtaaata taatcttttc
     2281 tgtatgaaat aatcttctta ttgtgagatt aaatatctgt aggctctctc tgcagagtct
     2341 ctgtttttac ttaatggtac ggcaatacta atagcgatgt tgcctgtggc tattcaatgc
     2401 tgacttttgt gaagatcaga ttatttcctg taagtaattg aatggtgctt tcttaaactc
     2461 aattatatga ggtgtgtttt ttattgtaat tcgttctggt cctcagttta agagagtctt
     2521 actgtattct gaatcattac cctattactg taatgaggta atac
//
LOCUS       DAZGJW010000340         2545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZGJW010000340 DAZGJW010000000
VERSION     DAZGJW010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.343"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            235..600
                     /locus_tag="ACHKIK_005277"
     CDS             235..600
                     /locus_tag="ACHKIK_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO9086219.1"
                     /translation="MSDMTISLPKEVNGHVICSTRIRHILAIRKSILMQIYMLFRQQA
                     GISALRQELEVKTPYRAMNHPVIGVVTKADLASMEHISLVKCWLREAGAHNVLVTSAV
                     NNNGVTELFALLHTEEGCC"
     gene            complement(701..1030)
                     /gene="tmaR"
                     /locus_tag="ACHKIK_005278"
     CDS             complement(701..1030)
                     /gene="tmaR"
                     /locus_tag="ACHKIK_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO9086220.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1202..2260)
                     /gene="yeeA"
                     /locus_tag="ACHKIK_005279"
     CDS             complement(1202..2260)
                     /gene="yeeA"
                     /locus_tag="ACHKIK_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO9086221.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLIDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(2458..>2545)
                     /locus_tag="ACHKIK_005280"
     CDS             complement(2458..>2545)
                     /locus_tag="ACHKIK_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase inhibitor"
                     /protein_id="HFO9086222.1"
                     /translation="CFEVYLNNGAEDGYWDIEMYVAVQPKHH"
BASE COUNT          705 a          614 c          589 g          637 t
ORIGIN      
        1 cctactgatt gctaaggata aacaagtatt aaaggccaga ctgataagac cgcgactaag
       61 tgaacctgta tctgacatgg catagcttcg ctaatgtgtc atcatgatta ggactaaatc
      121 tgtgaattcc tttaggaagc acggacacag gccaatctga gtccatatgt atgattggaa
      181 tcttacacag atgacttttt gacacgggga cttggcaaac aggggcgacc atgtatgtca
      241 gatatgacaa tctcacttcc gaaagaagtc aacgggcacg ttatctgttc aaccagaatc
      301 cgacacattc tggctatacg aaagagcata cttatgcaaa tctatatgct tttccggcaa
      361 caagcaggaa tctcagcgct gaggcaggaa ttagaggtaa aaacgcccta ccgggccatg
      421 aatcacccgg tcattggagt agtaaccaaa gcagatttag ccagcatgga acacatatct
      481 ttggtgaaat gttggttacg ggaagctgga gcgcacaacg tgttagtaac cagtgcggtt
      541 aataacaatg gagttacaga actttttgcc ttgctgcata cagaagaagg ctgttgctaa
      601 tttcgatagg ttctcagatg agaatgatga cggtgataca cagataagaa tcaatgagca
      661 atgagggttg ccgggcaacc ctcattgaat aaaacaggaa ttacttcgct tcgccgtttt
      721 tcatttcgcc catagctttc agctttttgg agatatcgcg gcgttctttg gagagctcgg
      781 catttttgat gatgtaatcg tcaacgcgat cttcatagtc acctttcatg ctggcgatga
      841 tgccctggat tgcttcaacg ctcatcccgg gcttgatgta atcgctcagg ttgtccagca
      901 acaggacgcg cttctggttg tcacggatct ttttctcaac gtcctgaatt tcacgttgca
      961 gtttgttctt acgacggaac agacgaacaa attccagtac gtcctggaat gaaggcttgg
     1021 tagtttccat ttttataccc ctgataatgt gagagtcgga ttcgtttaag caaccgctat
     1081 tcgttagggc caacattact catagctgcc gcagatgaca atgcttttat cccttttcac
     1141 tatcataccc tttatccttg ctgaatcgaa gcagcagcaa gatgattctg aagttcagga
     1201 attatttgcg caaggcccgg caaatcagac tcgatagcaa ctcaagctga tgtgccgttt
     1261 ccatgttcag ccacacataa ccatagattg gtgtttccac aaccttcagg tcatggtggt
     1321 tattcagcaa ctgacgcagc tcttccacag catcgatcaa tttttccgta ttggcaaaaa
     1381 ccggctgcgg attaccttcg tacagcgcat gaacaaggct cagcagtatt tgctgcatca
     1441 tgtgctgggt atcacgaagt ttttgcgcgt tcaataacac gaaatggctg gggcgcgtgg
     1501 cccagtatgc attgatttgc aactccagca tacaaaccag attgcggtta atggtctgga
     1561 taccttcata tatcgatttt ggaatacgag tttctttgct ggcgggcgca atcagcccac
     1621 gcattttcac ggcatcggtc aggagttttt gtagatggct ttccagacgt gggcgttcga
     1681 gtaagttcgg tgagaatgca gattgataaa cccgattata ctcggtcaga cttttcgcca
     1741 gttgaatgcg ccagtggatg aacgcccgtt gtggccagat accggtaaac aacattgcca
     1801 gtaaagagcc gaggatcaca tcgccgcttc gccataacgc cgtatcaatt tcacctgtcg
     1861 gggaacccac aacaattgcc agcgtcaccc caatcaataa tccttgatac ggtttcttgc
     1921 ccagcgccag ccaaccgcaa agaaacatag ccgccgcgca ccagactaac atcagcggta
     1981 acgaaattaa ctccagttgc agagcgataa gacctaaaat cgaacccaac accgtaccgc
     2041 caatacgttc gaaggcgcga gggacaacgt taccccagaa cgagattggc cccataatca
     2101 ccaccatggt gaccagcggc caggtgcttt ccgggatagt aaacaggcgg ataatgagaa
     2161 aagtgagcag gaatgccagc gcgacccgag taccatgcac aatgcggtaa tggcggtata
     2221 cccggatttc aaacgggctt aatgacttat cggcacgcac gcagaaactc cgtaatgaag
     2281 attaaaagtg ctcataactc atctgtcggc cagaaggccc gcctctgaat tatccgacgg
     2341 acaaaccggg aacagtgtaa tttcccggca ggagcgaaaa aattgaaagg cgcatcttac
     2401 tcttttcgct ttcaaaaaaa gagtggtcat cgcgttaaca caccgccctg agatgaatta
     2461 gtgatgtttt ggctgcaccg caacatacat ttcgatatcc cagtacccat cttccgcgcc
     2521 attgttcaaa taaacctcga agcag
//
LOCUS       DAZGJW010000341         2531 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZGJW010000341 DAZGJW010000000
VERSION     DAZGJW010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.344"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..666
                     /gene="tnpA"
                     /locus_tag="ACHKIK_005281"
     CDS             <1..666
                     /gene="tnpA"
                     /locus_tag="ACHKIK_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO9086223.1"
                     /translation="QTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTIHTL
                     FQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRASYSREFKV
                     RLAKQALQPGAVVARIAREHGINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDTP
                     EPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIREMKG
                     GTR"
     gene            663..1010
                     /gene="tnpB"
                     /locus_tag="ACHKIK_005282"
     CDS             663..1010
                     /gene="tnpB"
                     /locus_tag="ACHKIK_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9086224.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            1030..2531
                     /locus_tag="ACHKIK_005283"
                     /pseudo
     CDS             1030..2531
                     /locus_tag="ACHKIK_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          638 a          688 c          707 g          498 t
ORIGIN      
        1 cagacaaaaa aagatattcc ctgcttccgt tcttatttgc ctgatgctct gcgtttaaga
       61 tttgaagata aactgaccat ccgggccatc gctcagcgtc taggtctcag tcattccaca
      121 atacatacgc tttttcagcg atttattgca tccggtatcg catggccatt gcccgattca
      181 gtttcattag ctcagcttga tgccatcctt tatgccaaca gaaagaagga attaacagag
      241 cctcaaatca gcgaaggcac atggcgaaaa gaacggcgag ccagctacag ccgtgaattt
      301 aaggtccgtc tggctaagca ggcattacag cccggtgctg ttgttgcccg gatcgccaga
      361 gagcacggta tcaatgataa cctgctgttt aaatggaaaa gccagtacga ggacggctta
      421 ctgagcgatg atgacataca ggaatgcatg cctgtcccgg tggctctgac tgatacgcca
      481 gagccgacca gaccagttac aaatcccttc tggcgtaaca agcctgatga gtgccctgag
      541 agtgatcccg gaaacgtccc acggtgcgag ctgcatctta aatcaggtgt ggtaaaactg
      601 tttgaccctc tcactccgga aatgttacgg gcgctaatcc gcgaaatgaa aggaggtacc
      661 cgatgataac gctgccaacc ggtaccagaa tctggatcat cgctggcatc acagatatgc
      721 gttgtggctt caacggcctg gcttcgaagg tgcagaacac gctgaaagat gacccgttct
      781 ccgggcatat cttcgtcttc cggggccgca gtggcaaaat ggtgaaaata ctgtgggccg
      841 atcgtgacgg gttatgcctg ttcgccaaac gcctggaacg gggccgcttc gtctggccgg
      901 taacccggga agggaaagtg cacctgacgc cagctcagtt atccatgcta ctggagggga
      961 tcgcgtggca acatcccaaa cggacagaac ggcctggcat ccggatataa cccgtgataa
     1021 aacaggggaa tgaacaacac actccccgac gacatcgagc aactgaaggc cctgctgatc
     1081 gcacagcagg ctgttatcgt ctgtctggtg aaacaaccgg ctatgcccgc gagatcagct
     1141 cactcagagc gctggtcgct aaactgcaga gaatgttgtt cggtcgcagc agcgagaaaa
     1201 gccgcgagaa gatagaaaag aagatcgcac gggcagaaac gcgtataacc gagctccaga
     1261 acaggcttgg tgaggcgcag ttgcaactca cctcaatggc cggagagaca gcgccgaaaa
     1321 catcagactc tcccgtccgc aaagcacttc cggcaacact tccccgtgac aggcaggtta
     1381 tctccccggc agaaaccgaa tgccccgtct gcagcggcaa actgaaaccg ctgggagaaa
     1441 gcatctctga acaactggat atcatcaaca ccgcgttcag ggtaatcgaa acggttcgcc
     1501 caaaactggc ctgcagccgg tgcgactgta tagttcaggc tccgcagcca ccaaaaccca
     1561 tcgagcgcag ttacgccagt ccggctctgc tggcccgcat aatcatggct aagttcgccg
     1621 agcatctgcc gctgtaccgt cagtcggaaa tctatgcccg ccagggcgtg gagctgcacc
     1681 gcaatacgat ggggcgctgg gttgacatca tgggagagca gcttcgcccg ctgtatgatg
     1741 aactgaagca ctatgtgctg atggcgggta aagtgcatgc cgatgacacg ccggtaaatg
     1801 tactggagcc gggtcagggt aaaacccgta ccggacggct gtgggtctat gttcgtgacg
     1861 atcgcaacgc cggttcgacc atgccggcag cggtgtggtt ctcatactct cccgaccgca
     1921 aaggcatcca cccacagcaa catctggcgg actacagagg tatcctgcag gccgatgcat
     1981 atgcgggtta caatgctctt tacgaaagcg gtcaggtaac cgaagcggct tgtatggcac
     2041 atgcccgacg caagatccac gatgtacatg tccgccatcc aacgacagta acgggagaag
     2101 cgctccgtcg tatcggggaa ctgtacgcta tcgaggctga gatccgcggc agtccggcag
     2161 aagagcgact ggcggtcaga aaagccagaa cggtaccgct aatgcagtcg ttgtatgagt
     2221 ggctccaggg gcagatgagc acgctgtcgc gccactcgga tacagcgaaa gcgttcacct
     2281 atctgctgaa gcaatgggac gctctgaacg aatactgccg caatggctgg gtggagatcg
     2341 acaataacct gtgtgaaaac gccctccggg tagttgcact ggggcggcgt aactacatgt
     2401 tcttcggctc tgatggtgga ggcgagagtg cggcagtgat gtacagcctg atcggtagct
     2461 gcaaacttaa cggaatcgag ccggaaacgt ggctacgcca cgtgatcagt gtaatcaaca
     2521 cctggcctgc c
//
LOCUS       DAZGJW010000342         2521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZGJW010000342 DAZGJW010000000
VERSION     DAZGJW010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.345"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..882
                     /locus_tag="ACHKIK_005284"
     CDS             <1..882
                     /locus_tag="ACHKIK_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9086225.1"
                     /translation="FPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVM
                     RYAVQSGIIRYNPALDMAGALTTVKRQHRPALNLSRLPELLSRIDGYKGQPVTRLAVM
                     LNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSK
                     QAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAM
                     ACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRE
                     KGISPFEYANVNNPLRR"
     gene            1173..1526
                     /locus_tag="ACHKIK_005285"
                     /pseudo
     CDS             1173..1526
                     /locus_tag="ACHKIK_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1787..1969)
                     /locus_tag="ACHKIK_005286"
     CDS             complement(1787..1969)
                     /locus_tag="ACHKIK_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086226.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICFLQKVMIIDKTKYK"
     gene            1908..>2521
                     /gene="iha"
                     /locus_tag="ACHKIK_005287"
     CDS             1908..>2521
                     /gene="iha"
                     /locus_tag="ACHKIK_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO9086227.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRG"
BASE COUNT          668 a          575 c          626 g          652 t
ORIGIN      
        1 ttccccttta tcggcaaccg ggatatcaca acactcaata ccccggacct gcttatccct
       61 gttcgtgctg cagaagcaaa acaaatttat gaaatcgcca gtcgtctgca gcaaagaata
      121 tctgctgtaa tgcgttacgc cgtacagtct ggcatcatca gatataaccc ggctctggat
      181 atggctggtg cattgaccac ggtaaaacgc cagcatcgcc ccgctctgaa tctttcacgc
      241 ctgcctgaac ttctgtcgcg tattgacggt tataaaggcc agcctgtcac ccggcttgcc
      301 gttatgctga atttactggt ttttattcgt tccagtgaac tcagatatgc ccgctggtct
      361 gaaattgata ttgacaatgc catgtggact attccagccg aacgcgaacc tctgccaggc
      421 gtaaaattct cacaccgggg ctccaagatg cgaacaccac atcttgtgcc actcagcaaa
      481 caggctgtag ccatactgac agaactacag acatgggctg gtgaaaatgg tctgatcttt
      541 acgggagcac atgacccgcg taaaccaatc agtgaaaata ctgtaaataa agccctgagg
      601 gtgatggggt atgacacaac ccaggatgtc tgtggccatg gattccgggc gatggcgtgc
      661 agtgcattga ttgaatcagg tttgtggtcc cgcgatgctg tggaacgtca gatgagccat
      721 caggagcgta atggtgtacg tgctgcgtat atccataaag cagaacatct ggaagaacgt
      781 cgactgatgc tacagtggtg ggccgatttt cttgatgcga accgggagaa ggggatcagt
      841 ccgtttgaat acgcaaatgt taacaatccg ctgagaaggt gacagaaggc tattcacaca
      901 catgctcttc ccatggggaa gagcaacctg ctccaataaa tttctttttt attccggctg
      961 atagcaccgc aatcacccgt tacctctaaa tagccactta gcattcggcg tataagttga
     1021 cagagaagga gtatctcagc aggagcttaa agtgtggttt taggcagaat ttgattgatc
     1081 ctccatttgg ggcatgtgac taatcgatat agcttcctga acggaaaaag tgttttccct
     1141 tccaatataa actattcgac aataatataa atcaattaca gaataaaact ctgtataaac
     1201 atgcaaaaat ggtaaaggcg ttcgactata tcctgaatca ctggaatgcg ctgaacgagt
     1261 tctgtcgtga cggctgggtg gaaatagaca acaacatcgg tgaaaacgcg ttacgatcgg
     1321 tggcggttgg aagaaaaaat tatctcttct tcggctcaga caagggggga gaaagcgcgg
     1381 cgatcatcta cagtctgctg gtcacctgca aactgaacga agtggagccg gaggactggt
     1441 tgcgcgaagt gatcgtgaag ctcaatgact ggccgtcgaa ccgtgtgcat gaactgctgc
     1501 cctggaactg ctcaactgta aaataatcct tacgctacgt acttctcggg gcgcttacga
     1561 atttttcacg tgccacagaa ttttgaacgc tttctcttac aatatttcaa tgtttctatc
     1621 agtattcgcc ggaagaagtc atcgaccaaa tcatcccagt cgtctcgcac cactgaccat
     1681 tcatggttga catgtggtgg aaatccgctt ctacagtaac cattttttat tcgcaaaacc
     1741 gaacacgcca tacggatagc tgttaactgg catgccgagg cagtcgttat ttatatttag
     1801 ttttgtcaat aatcattact ttttgcaaaa agcaaatata aattattctc attattgttt
     1861 gtatttgtgt attgtcttgc cggttaacat gatcggagat tagtaatatg cgaataacca
     1921 ctctggcttc cgtagtcatt ccctgtctcg gattttcagc cagcagcata gctgctgcag
     1981 aggatgtgat gattgtctcg gcatccggct atgagaaaaa gctgactaac gcagccgcca
     2041 gtgtttctgt gattagccaa gaggaattgc agtccagcca gtaccacgat ctggcggagg
     2101 ctctgagatc agtagagggt gtggatgttg aaagtggtac gggtaaaacc ggtgggctgg
     2161 aaatcagcat ccgaggaatg ccagccagtt acacgctgat actgattgat ggtgttcgtc
     2221 agggcggaag cagtgacgtg actcccaacg gtttttctgc catgaatacc gggttcatgc
     2281 cccctctggc cgccattgag cgtattgagg ttatcagggg gccgatgtcc acactgtatg
     2341 gctctgatgc gatgggcggt gtggtgaata tcattaccag aaagaatgca gacaaatggc
     2401 tctcttccgt caatgcaggg ctgaatctgc aggaaagcaa caaatggggt aacagcagcc
     2461 agtttaattt ctggagcagt ggtccccttg tggatgattc tgtcagcctg caggtacgcg
     2521 g
//
LOCUS       DAZGJW010000343         2517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZGJW010000343 DAZGJW010000000
VERSION     DAZGJW010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.346"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            674..901
                     /gene="ykgL"
                     /locus_tag="ACHKIK_005288"
     CDS             674..901
                     /gene="ykgL"
                     /locus_tag="ACHKIK_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HFO9086228.1"
                     /translation="MQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(936..1076)
                     /gene="ykgO"
                     /locus_tag="ACHKIK_005289"
     CDS             complement(936..1076)
                     /gene="ykgO"
                     /locus_tag="ACHKIK_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO9086229.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(1076..1339)
                     /gene="ykgM"
                     /locus_tag="ACHKIK_005290"
     CDS             complement(1076..1339)
                     /gene="ykgM"
                     /locus_tag="ACHKIK_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO9086230.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVTSEGNVARFTQRFGRFVSTKKGA"
     gene            complement(1703..1804)
                     /gene="ykgR"
                     /locus_tag="ACHKIK_005291"
     CDS             complement(1703..1804)
                     /gene="ykgR"
                     /locus_tag="ACHKIK_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFO9086231.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            complement(2278..2367)
                     /locus_tag="ACHKIK_005292"
                     /pseudo
     CDS             complement(2278..2367)
                     /locus_tag="ACHKIK_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          743 a          456 c          552 g          766 t
ORIGIN      
        1 taagtttaat tattttcgat tgggcttaaa tttatgtttt tttcccgatg agtgtcaata
       61 tgtgaacgga aaaggaattc aatggttaag tcaatattta gaaaaactga aaatattgct
      121 tttttgataa gaagaaaaag acatttggtt tcataaggtt attggcaata taacgttcaa
      181 ttcttacttg cattttatat tggtcaggaa ctttgccctg aagtgttagg aatagtctta
      241 atgcgctgtg tgatggacta gtaaaaaaac aagttttaag ggtaagggat agaatataaa
      301 actataatga gctgtcattg aagatgattt gccaatcagt aagaaatttt aatggattaa
      361 catgccgcac aaaatggtta taggtgttca ttacaataag tgatggaaaa ttgaacgggc
      421 gtatgtacgt catctgttcg gtaagccccc aagagccaga ttaagtgaag ttaaacgaag
      481 gctgccagct gtcacaggaa cgatggcctc tttgtcaggg cactgagtgc agttatctgt
      541 gcgcgttact ctaactggca ctcgaacgtc ggctacgggt taccggtgga tcagactgtt
      601 cctgtggcgt aaacgttttt atgttagtac caacagattt accacattta tcatcttaac
      661 agtttgagtg aaaatgcagg ctgcgccgca aagggaggag actgagtggc gtgttcagtc
      721 taaaagaggg ttgatgccag catacagggg cgaagctggt caacaagtca acattaagat
      781 aatggaatat tcggagagaa acgtcaggca gttggcctca aatgaacagg aagaatatat
      841 ccccaggaaa attaatgttg gtgttataaa cacaccaaca ttaatccgat ctgattatta
      901 atcagacaaa tatcgttaat tcgtcgaatt ttgaatcaac gttttttctt acgaccctga
      961 acggccttaa aacgtggatt agatttacaa atcacataca accgtccttt tcgcttcaca
     1021 atctgacagt ctggatggcg ttcttttgcg gtacgcagag agttaagaac tttcatcacg
     1081 cccccttttt cgtgctaaca aaacgaccaa aacgttgggt gaatcgtgca acatttcctt
     1141 ctgatgtcac tgttctcagc ttccctgtat agaacgggtg cgatttagaa gagacatcaa
     1201 ttgtcacgta cggatacgtt acgccatcca gctcaatctc acggtctgtt ttgatagtcg
     1261 agccgatttt aaagtactca tcaacactgg tgtcgtggaa caccacagta cgatactcag
     1321 gatggatatt gggcttcatc atttttacct gttatgttat aacataaccg taaagataca
     1381 tgctcatccg gatcatttca acacccaatc cctgtgccca tttgtggtat gtggaatttc
     1441 cccaacgggg aagtctggca ttgttagcag ccgttggcga cgctcccata aacgcctgga
     1501 tgagtgattg atgatggttt tgctgagagt atggattttt cgagaatgga ggttatctcc
     1561 actttactgg ccgatgattt atcgcagtat gattgattac ctgacagcgt gtattcctct
     1621 cccacggata taataattac ttacaaaaag ggagaggatg catattttaa atatcactga
     1681 agtgaacagt ttattgccgt tattaataga aatggagaaa taaataggcg tattctacaa
     1741 ttgcgacaaa aacaacgata ttaatcagtt tatgactgat ttgttgtact ttattctctt
     1801 tcattggtac ttcctcgctt taaaaaagag tgcacttcgt aagtgccctt atataaataa
     1861 cgagtttggt caaccaattt tttgacatgt atcacaaatt tgaatagatg tattacatca
     1921 gctatctttt attgcaccaa cgtcattgat atatgtcgac tgaagtcagt tccgggaatg
     1981 agtctgatct caagactggc ccagtccggg cgttgattgg cgctgaggag catacctcat
     2041 ttcatcataa tgtcgtatct cctggggtgt tatacaagat atcgttgttg gtaacctggg
     2101 aaaggaattg agttctatta aaccgtcaac tatgccggat acatactgga ttacactgca
     2161 ggcacgcctt atgagagaac gtgccgcagt gacgggttaa ttatctgaaa gaatttgtga
     2221 ggctgtatcg gttactcatt gatttgatag ttttactctc gggagaataa tagatattta
     2281 atccattaac ggaaaccagc cagttccttt cgatgcctga atttgatccc atagtttatc
     2341 cggaatggtt aattcatgaa caagttgtgt tatcgttcat gagaagcata acgtaaaggg
     2401 aaaagctcga ttagacggca gaatttgtca ggggttatga acgaaattca taaatctgtt
     2461 tgagtgttgc gatgggtagt gcaagttcga tatctccgca atttacagtc cgatgaa
//
LOCUS       DAZGJW010000344         2477 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZGJW010000344 DAZGJW010000000
VERSION     DAZGJW010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.347"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            300..1277
                     /locus_tag="ACHKIK_005293"
                     /pseudo
     CDS             300..1277
                     /locus_tag="ACHKIK_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1800..>2477
                     /locus_tag="ACHKIK_005294"
     CDS             1800..>2477
                     /locus_tag="ACHKIK_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086232.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNLLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNKLLKAGKIAGNNNELLMEIKKESKSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNSLTPEN"
BASE COUNT          800 a          524 c          538 g          615 t
ORIGIN      
        1 tttgatcaca actgaagatg ctttttacaa tgaagaatta ttgttaaaaa acgctttgtt
       61 gaatcaaccg aactgttgct gagttgcagg atcacatcac gtattttcct gaaaacgcag
      121 gctgcttagt agcaaagcag aagttcaaaa tcaccaacta aatagctgcg ccgaatagta
      181 gatcaatgaa agggaactca gcccggattg tgcgatctga tcaatcgcca aaaatccccc
      241 cctaaccgga ctgagcgatg ccgatcatag caccaattcc ccgcgacgaa tgacgcctga
      301 tgcaaaaagc aatctataaa actcacgata aaaattatgc ccgcaggctg accgccatgc
      361 tgatgctgca ccggagcgct cgcgtcagtg acgttgccag aacgctctgc tgcgcccgtt
      421 cctccgtcag gcgctggatt aactggttca cgctgtcgag tgttgaaggt ctgaaatcat
      481 tacccgcagg acgttcccgc cgctggccgt ttgagcatat ctgcacattg tttcgtgaac
      541 tgataaaaca ctctcccgga tattttggct atcagcgttc acgctggagt acagaactgc
      601 tggcaataaa aatcaacgag ataaccgagt gccagttaca tgccggaacc gttcgccgct
      661 ggttgccatt agtcgggctt gtgtggcgca gggctgcgcc aaccctgcgt acccgtaacc
      721 cgcataaagc agcaattcac aaagcactgg acgaatgtag tgcggagcat ccggtctttt
      781 atgaagatga agtgtatatc tatcttaatc ccaaaaccgg tgcggactgg caacttcgca
      841 gacagcaaaa acatgtggta acaccggggc agaatgaaaa atactatctg gctggtgctt
      901 tacatagtgg aacgggcaaa gtcagctatg tgggtggcaa cagcaaaagc tcagccttat
      961 ttatcagtct gttgaaacat ctgaaattga cataccgacg ggataaacct atcacgctca
     1021 tagtggacaa ctacatcatc cataaaagtc gggaaacaca gcgctggctg aaagaaaatc
     1081 cgaagttcag agtgatttat cagccagttt actcgccgtg ggtgaatcat gttgagcggt
     1141 tatagcaggc gcttcacgat acgataacgc gcaatcatca gtgccgttca atgtggtaac
     1201 tgcttaaaaa ggttcattat tttatggaaa ccgtgagccc attccccggt ggtaaatatg
     1261 ggctgttaaa agtgtagcgg tattcggcgc agctatttag gatggtcagg tcgcagaagt
     1321 tctgtgccac tacagtgcgt gtactaaaca aaatgacgag ggcacgaatg tcataatacg
     1381 tgcgtattac ctgcagtagc taccaactga gggggcttat cccaaacctg atttattcaa
     1441 caaagctgga ctggacttat ccttactctg aattattttc cagacatatg catcatgtac
     1501 tggctcgctt ggttcaggaa atacaggagc aaatctacag ctattagatt tattcctatt
     1561 tgtatgcaaa gtcgcattgc ataatgcgga gctatgttac agatacgcct gacgtgcgta
     1621 gtaaaattat aaagcaacat atatatacaa ggagaaaccg agaaaaataa atgagtcgta
     1681 tgttgggtag catcgagata aatatttttt tatcacatta gcaccttggt taagggtgtt
     1741 aataataaga ttttctcact tattaaaaca taaataattt tttaagcgga aatgtaatta
     1801 tgagactgcc agagaaagtt ctttttcctc ctgtcactag tggcctgtca gggcaggaaa
     1861 aacaaaaaaa accgaagagc attaccggat ttcaggaaaa ttatcaacgc aatatcaggc
     1921 caatcaaaac agcatcagaa gcccgactac gcttctttga taaaatggtt tcgaaagaaa
     1981 actctctgga agatgttgtt tctttaggtg aaatgattca gaaggaaatt tatgggcatg
     2041 aacaaagaac attttcacca gttcatcata caggtaactg gaaatcatca ttgttacaca
     2101 acgcgctcct tggtctggca aatgtttata atggcttacg ggaaacagaa taccctaaca
     2161 ctttcaacag agatggtata aaaagtacta actcttttag agataactta ttgacaaaaa
     2221 caagaactcc cagagataat tttgaggaag gaataaaaca tcctgaacat gcaacaatac
     2281 catatgacaa cgacaatgaa agtaataaat tgctaaaagc aggaaagata gctggtaaca
     2341 ataacgagct gttgatggaa ataaaaaagg aatccaaaag cgaccatcaa atccccctgt
     2401 cagataagtt cctgaaaagg aaaaaacggt ctcctgtagc tgaagataaa gttcaaaact
     2461 cgttaacacc agaaaat
//
LOCUS       DAZGJW010000345         2473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZGJW010000345 DAZGJW010000000
VERSION     DAZGJW010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.348"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(45..191)
                     /locus_tag="ACHKIK_005295"
     CDS             complement(45..191)
                     /locus_tag="ACHKIK_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086233.1"
                     /translation="MIFIASSSREQTTASDGKMGTTGYSGNLAEEAYGTDCNVKYKTL
                     KIPF"
     gene            619..897
                     /locus_tag="ACHKIK_005296"
     CDS             619..897
                     /locus_tag="ACHKIK_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFO9086234.1"
                     /translation="MPFSIKSIFSGHTWHQPEISRPIADKSSTKNCILDSTTCNVDGF
                     TVFNRRSCSFDMRPPGSADRTPQLRLSISEVAWMSKIIETETNNTNKS"
     gene            1435..1596
                     /locus_tag="ACHKIK_005297"
                     /pseudo
     CDS             1435..1596
                     /locus_tag="ACHKIK_005297"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1580..1645
                     /locus_tag="ACHKIK_005298"
     CDS             1580..1645
                     /locus_tag="ACHKIK_005298"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HFO9086235.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            1761..2018
                     /locus_tag="ACHKIK_005299"
     CDS             1761..2018
                     /locus_tag="ACHKIK_005299"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9086236.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(2065..>2473)
                     /gene="nleF"
                     /locus_tag="ACHKIK_005300"
     CDS             complement(2065..>2473)
                     /gene="nleF"
                     /locus_tag="ACHKIK_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HFO9086237.1"
                     /translation="CARNNNELEEIFQELSAFVSGLMDKRNSVFEVRNENTDEVVGAL
                     RAGMTIEDRDSYIRDLFFLHSLKVKIEESRQGKEDSKCKVYNLLCPHHSSELYGDLRA
                     MKCLVEGCSDDFNPFDIIRVPDLTYNKGSLQCG"
BASE COUNT          759 a          541 c          478 g          695 t
ORIGIN      
        1 aaaatagtag attagatcga acttatatta cagctattta gtttctaaaa tggaattttt
       61 aacgttttat acttcacatt acaatctgta ccataagcct cctcagcgag gttgcctgaa
      121 taccctgtcg tgcccatttt tccatcggac gcagttgtct gctctctcga actgctggca
      181 ataaaaatca accagacagc ttgatgtccg ttacatgcag gactaatcac gattggttac
      241 cgtttacaaa acttgcgtaa cattggagga gaacgaataa tattctctag ctggtacgcg
      301 ttccatagcg aaacgggcaa ggtccaacag caagagctga aactcagggg gatttatcag
      361 tcgatttatt cgccatggat gaatcacgct gtacggttat ggtagctctt agcgacaagg
      421 cataatcatc agtacaccaa tgtaacaacc gttgaaaaag actcattatt ttatgaacca
      481 gccatcccat ttccagagtg gtaaatatga tttgttaaag tgtaacagta ctctgcgtag
      541 cctttttaga ttcatagttt aatgtttcta aatacattaa attgcactat aagagaaaga
      601 cataatgtga ggataaaaat gccattttca ataaaaagta ttttttcagg acacacttgg
      661 catcaacctg aaatatctag gcctatagca gataaatcat ctactaaaaa ttgtatctta
      721 gactcaacaa catgcaatgt agatggcttt acagtgttta acagaagatc ctgtagtttt
      781 gatatgcgtc ctcccggaag cgcagacaga actccacaac tgagattatc aatatcagaa
      841 gtcgcatgga tgtcaaaaat aatagagaca gagacaaata atacaaacaa atcatagcta
      901 ttagcccaga aaagagttgg tacactagcc atgaataact tcctaacaac ttgatttttt
      961 agttattttt ttcttcattc ttgaaaaagg atgccccgaa agggttccag agttcgattt
     1021 tctgcgcttc tgcaaataat caaggggtta caaagtggtt gcccctttgt ttttagtgct
     1081 cttggtatac ccattggtac atttagcgca ataccatctg gtatcactta aagacacgaa
     1141 aaacaacttt ttatcttttt gtttcgctca aattagtata aaaagccgaa caacaaacaa
     1201 taaaaaacca ttaacatcaa tagattatga tggcttcaat ctaaataata ggctatataa
     1261 tactgcaaga cacaacacat gctgtcacta tgagtcaact atttagatga tattagtgac
     1321 ctgtttcaga gcattagcgc aaggcgattc tttgtcttct tgcactaatt ttttatcata
     1381 aaaatgttcc tagcactggg catcaatatc gcaggtcaga aagagctcct ggggatgcgg
     1441 ctggccgaaa atgaaggggc gaatttctgg ttcaatgtgc tgactgaact gaaaaaccgc
     1501 ggtctgaacg atatcctcat cgcctgtgtg tatggcctga aagaattccc ggaggcccgc
     1561 atccagttat gcatcgtgca tatggtgcgc aacagcatgc gcttcgtgtc atggaaggaa
     1621 tacaaagccg tcactcgcga cctgaaagcg attagcctcc cacagaagag gcaggccagc
     1681 aggcactgga agcgtttgct gcggcctggg actgccgcta tccgcagata agccggtgct
     1741 agctgtcaaa ctggactaac ttggcgacgt ttttcgctta tccggcagat atccgcaaag
     1801 tgatctacac aacgaacgcc atcgagtcgc tgaacagtgt gatccggcat gccatcagga
     1861 aacgcaaggt gttcccgacg gacgagtcag taaaaaaagt ggtgtggctg gcaatccagg
     1921 ccgcgtcaca gaaatggaca atgccgttaa gggactggcg aatggcaatg agccgcttta
     1981 ttatcgagtt tggtgaccgt cttgacggtc acttttaaga aaaggcattt acacagaatc
     2041 ctaaacaggc tcacaggcct ctaatcatcc acattgtaaa gatcctttgt tgtaagtaag
     2101 atctggtacc ctaataatat caaaaggatt aaaatcatca ctgcatcctt ccacgaggca
     2161 tttcattgct cgtagatcac catatagctc tgaagagtga tgcggacata gcagattata
     2221 aactttacat ttcgaatctt ctttgccttg tctactttcc tcaattttta ctttcaatga
     2281 atgcagaaaa aaaagatccc tgatataact atccctgtcc tctatcgtca ttcccgccct
     2341 cagcgctccg acaacctcat cagtattttc atttctcacc tcaaatacac tatttctctt
     2401 atccatcagc ccacttacaa aagcacttag ctcttgaaaa atttcctcaa gctcattatt
     2461 atttctggca cat
//
LOCUS       DAZGJW010000346         2440 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZGJW010000346 DAZGJW010000000
VERSION     DAZGJW010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.349"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            41..550
                     /gene="eco"
                     /locus_tag="ACHKIK_005301"
     CDS             41..550
                     /gene="eco"
                     /locus_tag="ACHKIK_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO9086238.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(699..2345)
                     /gene="mqo"
                     /locus_tag="ACHKIK_005302"
     CDS             complement(699..2345)
                     /gene="mqo"
                     /locus_tag="ACHKIK_005302"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO9086239.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          576 a          669 c          608 g          587 t
ORIGIN      
        1 atgttaacct tcagcgacat catcggtgaa aacctataaa ttgaagaagg aaagcaaaaa
       61 aatgaagacc attctacctg cagtattgtt tgccgctttc gctaccactt ccgcctgggc
      121 ggcagaaagc gtccagccac tggaaaaaat cgcgccttat ccacaagctg aaaaagggat
      181 gaagcgtcag gtgattcagt taaccccgca agaagatgaa tctaccctga aagtagaact
      241 gttaatcggt cagacgctgg aagtcgattg caatttgcat cgtctcggcg ggaagctgga
      301 aagcaaaacg ctggaaggct ggggctatga ttattatgtc tttgataaag ttagctcgcc
      361 ggtttccacg atgatggcct gcccggatgg caagaaagag aagaaatttg tcaccgcgta
      421 tctgggcgat gctggaatgc tgcgttacaa cagcaagctg ccgatcgtgg tgtatacgcc
      481 agacaatgta gatgtgaagt accgcgtctg gaaggcggaa gagaaaattg acaacgcggt
      541 agttcgctaa aatgctgtga agtgcggcac cccgtaggcc gaataaggcg gtcacgccgc
      601 atccgacatc taacgcccga gccggtgcct gatgcgacgc tggcgcgtct tatcagcata
      661 cgccacatcc ggcataccat gccggatgtg gcacatcatt acaacgcaat atccgccact
      721 tctttttgaa cgggttgcgg tttcaactgc ggttttggcg tactatctgc tgcctgcggc
      781 ttgtcgtatt tcagccccag cacttcgctg gtgtactgca actcgcgttc tgttgccgct
      841 acatcaccgt tcagcttgcg tccataagac ggaacgatcg ctttcaacgt agcctgccat
      901 tgcgggctgg aaacgcgatc gccaaatact ttttccagca gatccaacat aatcggcgcg
      961 gcggttgacg cccctggcga tgcccccagc agcgcggcaa tggtcccttg ctggtcactg
     1021 accacttcag tacccagacg cagtacgcca cctttctcgg catcacgctt gataatctgc
     1081 acgcgctgcc ccgcttgcca caaacgccag tcctcttttt ttgcctgcgg atagtactct
     1141 ttcaacgctt caaaacgatc ctcttcactc aacatcacct gactcaccag atatttcacc
     1201 agatcgaaat tatccagccc gacgtgcatc atcggcatca cgttagaggt ggtggtggaa
     1261 ctcatcagat cccacaatga accgtttttg aggaatttgg ttgagaaggt ggcaaatggc
     1321 ccaaacagca ctacgcgttt accgtccaga acgcgggtat cgatatgcgg aaccgacatc
     1381 ggtggtgcgc caacggatgc tttaccgtaa accttcgcca gatggtgatt aaccacgtcc
     1441 gggttttccg aaacaaggaa ctgtccgccc accgggaaac cggcgtagtc tttcgcttcc
     1501 ggaatccccg attcctgtaa tagcttcagc gccgcaccgc ccgcgccgat aaagacaaat
     1561 ttggcgcgga tgttctgtgc agtgccgttt ttcagatcgg caacggtaac ggtccaggtg
     1621 ttatcgtcat tacgctttag ggcgcggact tcgctgctga gttgcagcga gaagttagat
     1681 ttcttctgta aggaagcaat taactggcgg gtgatctcgc catagttcac atcagtgcca
     1741 atttctgtgc gtgttgccgc tactttctgt tgtggatcgc gcccttccat cactaacggt
     1801 gcccactctt tgatctgcgc gtgatcttca gagtaacgca taccgcgaaa cagcgagctt
     1861 tgttgcaacg cggcgtaacg ggcgcgcagg aaattaacgt tatcctcgcc ccagacaaag
     1921 ctcatatgcg gaacggtatt gataaatgaa cgcggagtac gcagcacgcc gcgctcaacc
     1981 tggtgcgccc agaactggcg ggaaatctga aatgcttcgt taatggcgac tgctttttca
     2041 atactgatgc tgccatcggc gttttgcggg gtgtagttca gttccatcag tgcagaatgc
     2101 ccggttccgg cgttattcca gccgttcgaa ctctcctgcg cgacaccctc caggcgctcc
     2161 accatggtca tcgaccattc aggctccagc tcgcgtaaat aggtccccaa cgtggcgctc
     2221 ataatgccgc cgccaatcaa cagtacatca gtttcctgct cctcggacgc tttcgctttt
     2281 gccgccatcg aaacggcatt aagccccacg gccatcgaga agagcatggc agtcactttt
     2341 ttcatcttgt taatgcctta cttttagtcg ctttattgca ggtgagattt gcgcggcatc
     2401 aacggtaaca cttaagtaac aacatttaaa taatgtttat
//
LOCUS       DAZGJW010000347         2415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZGJW010000347 DAZGJW010000000
VERSION     DAZGJW010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.350"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1997)
                     /locus_tag="ACHKIK_005303"
     CDS             complement(<1..1997)
                     /locus_tag="ACHKIK_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9086240.1"
                     /translation="MSQPVGDLIIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTATAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMLRGLAGAITLPMVGVTSLAVATGALAYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANGKISQQRRDL
                     WKAENQYAVLKEAATKRQLSEQEKSLLTHEKETLEYKRQLAELGDKVEHQKRLNELAQ
                     QAARFEQQQGAKQAAISAQARGLTDRQVQRESEEQRLRDVYGDNPDALAKATSALKNT
                     WSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSVATQTF"
     gene            complement(1978..>2415)
                     /locus_tag="ACHKIK_005304"
     CDS             complement(1978..>2415)
                     /locus_tag="ACHKIK_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO9086241.1"
                     /translation="FALRLAREMGRPDWRAMLAGMTSTEYADWRHFYRTHYFQDTQLD
                     MHFSGLMYAVLSLFFCDPDMHPSDFSLLVPRHEEEQVERLDEDKMLMQKAAGLAGGVR
                     FGGDGGGDILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
BASE COUNT          457 a          804 c          599 g          555 t
ORIGIN      
        1 tcaaaggtct gcgtggccac gcttttaacc tgcgaaaaac tgtccgtcgc actttctgcc
       61 cactcgcccc agccggactt cagaccggcc atccagcttc cacgaagctg ctcctccgca
      121 gaccaggtgt tcttcagtgc agatgtggcc ttcgccagcg catccggatt atcaccgtac
      181 acgtcacgaa ggcgctgctc ttccgactcc cgctgcacct gacggtcggt gaggccccgc
      241 gcctgggcac tgattgccgc ctgcttcgcg ccctgctgct gctcaaaccg cgcagcctgc
      301 tgtgccagct cattcagccg tttctggtgt tcaactttgt ctcccagctc agccagctgg
      361 cgtttgtact ccagcgtctc tttctcatgg gtcagcaggg atttttcctg ctcagataac
      421 tgccgtttcg tggctgcctc tttcaggacc gcatactgat tttccgcttt ccataaatcg
      481 cgacgctgct ggctgatttt cccattcgca ccgctgtgtt tttccagcgt ccggagctcg
      541 gtttcaagcg ccagcagggc agcatgcgcc tggtcttcct gacgctcacc ggctgacact
      601 ttgactcctg acgacttcgg ctttttcagc gtcgattcat aatccttttt cgccgccgcc
      661 atcagcgtgt tgtaatccgc ctgcaggatt ttcccgtctt tcagggcctt attcagttct
      721 tcctgacggg cggtatattt ctccagcggc gtcagcagac gctcatacgc cttctgcgcc
      781 tctccggtat acttcagctg tgatgcgtcc cgttcggccc ggtccctggc ggccagttca
      841 ccggcttttt ccatatccga ctgcagcgtg gccgctgcca gccccagacg ggcattttcc
      901 cggtcattcc atgcgccctg aaggttggcc cggaaagagg aggttttacc gcggcgctgg
      961 ctccgactct ggtaccactg ccatttttta tccgcctcat caaatgcctt ctgcgcactg
     1021 gcgagcatat ccgctgagga ttccggacga ccgatatcca gaatggcatc ccacatcgat
     1081 ttgaatgcct tccctgtttt atccgcccag gtctccagtg ttcccatgtt ttctttcagg
     1141 cgacgggtct gctcatcaaa gcctttcgtg gcgatatcgt tcgccgcctg caatgccccg
     1201 gcctcgtctc cggaacgctg cagctgtgca acatacgcaa tctgctctgc cgtcacgtta
     1261 cggaactggc gcgccatcgc catcagtccc gacgtcgggt cagtggtcag cttcccgaag
     1321 gcttcagcga ctttatccac ctccacaccg gatgcagacg caaaacgcgc gacactctgg
     1381 ttgatggcat caaactgttc accaccacgc acaccggcat tcaccagggc tgccagtgac
     1441 tctctcgcct ggttaaacgt cagccctgct gcctgcccgg ctcttgagag agtcagcata
     1501 cgatcggcag tcagtccgga ctgattaccg gaaagaacca gggttttatt aaacgctgaa
     1561 agcgtggaat ccccctggta ccaggcgtac gccagcgcac ctgtcgccac cgccagcgag
     1621 gtgaccccga ccatcggcag ggtgatcgca ccggcaagcc ccctgagcat ggggatcatc
     1681 ccgccgaagg agtccttcac ctgaccgccc tgttgcagca ggatcagcca gggattctga
     1741 ccaccggcaa gctgcgtggc gatatccgta aactgtgcgg gcagggtgcg catggccgct
     1801 ttatactgtc cgacggaaat cccggctttt tgcgcagcca gcgcctgacg gctcaggccc
     1861 tgttcaacag cagtggcggt ttttctggcg tcagtatcca gtcctgaaaa atgacgcctt
     1921 acccggctca tctgctcatc gaaacggacc gcatccagac tcaggtcaat aataagatca
     1981 ccaaccggct gggacatatc tcacacctcc cggaatcccc gctgaagcca tcattaatgc
     2041 ggcatcatcc accatgacat ccgccacatc cgcagacgat aaaatatcgc cccctccgtc
     2101 cccaccgaac cggacgcctc cggcaagtcc tgccgctttc tgcatcagca ttttgtcctc
     2161 atccagcctc tccacctgct cttcctcatg ccgggggaca agcagactga aatcagaggg
     2221 atgcatatcc ggatcgcaaa aaaacaggct gagtacagcg tacatcagcc cggaaaaatg
     2281 catatccagt tgggtatcct gaaaataatg cgtgcggtaa aaatgtcgcc agtcggcata
     2341 ttcggtggat gtcatcccgg caagcatggc gcgccagtca ggcctcccca tctctcgcgc
     2401 cagtctgagg gcaaa
//
LOCUS       DAZGJW010000348         2411 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZGJW010000348 DAZGJW010000000
VERSION     DAZGJW010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.351"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..226)
                     /locus_tag="ACHKIK_005305"
     CDS             complement(<1..226)
                     /locus_tag="ACHKIK_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086242.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQ"
     gene            complement(235..327)
                     /locus_tag="ACHKIK_005306"
                     /pseudo
     CDS             complement(235..327)
                     /locus_tag="ACHKIK_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            complement(360..698)
                     /locus_tag="ACHKIK_005307"
     CDS             complement(360..698)
                     /locus_tag="ACHKIK_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9086243.1"
                     /translation="MLMVKKTIYVNPDRGQNRKVSDRGLTSRDRRRIARWEKRIAYAL
                     KNGVTPGFNAIDDGPEYKINEDPMDKVDKALATPFPRDVEKIEDEKYEDVMHRVVNHA
                     HQRNPNKKWS"
     gene            complement(701..1006)
                     /locus_tag="ACHKIK_005308"
     CDS             complement(701..1006)
                     /locus_tag="ACHKIK_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFO9086244.1"
                     /translation="MVTIVWKESKGTAKSRYKARRAELIAERRSNEALARKIALKLSG
                     CVRADKAASLGSLCCKKKEEVVRKNRSIYYKDSNPLGNKIHAVQKIKLYSKLPYGAY"
     gene            complement(1321..1971)
                     /locus_tag="ACHKIK_005309"
     CDS             complement(1321..1971)
                     /locus_tag="ACHKIK_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO9086245.1"
                     /translation="MNTQLMGERIRARRKELKIRQAALGKMVGVSNVAISQWERSETE
                     PNGENLLALAKALQCSPDYLLKGEDSLSNIAYHSRHDPRGSYPLISWVSAGCWMEAVE
                     PYHRRAIDNWYDTTVDCSEDSFWLDVKGDSMTAPAGLSIPEGMIILVDPEVEPRNGKL
                     VVAKLEGENEATFKKLVIDAGRKFLKPLNPQYPMIEINGNCKIIGVVVDAKLANLP"
     gene            2052..2237
                     /locus_tag="ACHKIK_005310"
     CDS             2052..2237
                     /locus_tag="ACHKIK_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO9086246.1"
                     /translation="MYKKDVIDHFGTQRAVAKALGISDAAVSQWKEVIPEKDAYRLEI
                     VTAGALKYQESAYRQAA"
     gene            2347..>2411
                     /locus_tag="ACHKIK_005311"
     CDS             2347..>2411
                     /locus_tag="ACHKIK_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086247.1"
                     /translation="MAQASYSKLTQREIDRAETDLL"
BASE COUNT          618 a          547 c          479 g          767 t
ORIGIN      
        1 tttgtttcca ttcctgtaac cactgatttg gtgctggttt aaaattaaca atccaatgcg
       61 caggaaccaa ccatgcataa tgctctgtct gatgaaaagc tatatattga agtgcgaata
      121 tttttatccc atcttcttca actgtcgcct ggaatctcca gaaaacaggc attccatcat
      181 gttcagtttc tgattcagga aaaggtacgc tccatgattt tgtcatatct cacctcaaat
      241 aagtggtttg ctgcctaatt tcattttctg gcgaccaaca caagtcacac ccatttcact
      301 gcgtggcttg ctgtaataaa ttaggttagt tcagccaata aaaaacccac cgaagtgggc
      361 tatgaccatt ttttatttgg atttcgttgg tgagcgtgat taacaactct gtgcattaca
      421 tcctcatatt tttcatcttc aattttttcg acatcgcgag gaaatggtgt tgctaatgct
      481 ttgtcaactt tgtccattgg gtcttcatta atcttatatt caggaccgtc atctatagca
      541 ttaaatccag gtgttacacc gttttttaat gcatatgcta tcctcttttc ccatctcgct
      601 attctcctcc tgtctcgaga tgtaagacct ctatcagata cttttctgtt ttgtccgcgg
      661 tcaggattaa cataaatagt ctttttcacc ataagcatac tcaataagca ccgtacggta
      721 gtttactgta caattttatt ttttggactg catgtatttt gtttcctaat gggtttgaat
      781 ccttgtaata aatacttcta ttttttcgaa caacttcttc tttcttcttg cagcaaaggc
      841 ttcctagtga tgctgctttg tctgctctga cgcaaccaga gagctttagc gcaatttttc
      901 gcgccagtgc ttcattactg cgtcgctcgg caataagttc tgctctgcga gctttgtagc
      961 ggctttttgc cgtacctttg gattctttcc agacaatggt taccatgatg gtctccttta
     1021 agtggctttg gcgcatgacg cgtcgaggtg cttatcttct cgatcgctgt cttgcagctg
     1081 caattcgcgc catccccaaa accactcaag ttctggtctc aacggttagg ttgagagttc
     1141 gtcgatgtta aagagcctgc caatctgttc cgtttggctt ccagcctcct gctgacggct
     1201 taaaattaag acctcttaat tttttggtca agtgcatttt tgaagaaaac ttaattttat
     1261 gggcgcgaat ttagtttgtc tttgattttt aacgggaaat aaaaaagggg cgaaagcccc
     1321 ttaaggaagg tttgctagct tggcatcaac gacaacgcca atgattttac agttcccatt
     1381 gatttcaatc attgggtatt gtggattgag tggtttcagg aattttctac cggcatcaat
     1441 aactaacttt ttgaatgtcg cctcgttttc tccttcaagt ttggcgacta ccagctttcc
     1501 attacgtggt tcgacttctg ggtcgacgag aataatcatc ccttcaggaa tactcagtcc
     1561 tgccggggca gtcattgaat cacctttaac gtcgagccaa aaagagtctt cagaacaatc
     1621 taccgttgtg tcgtaccagt tatctattgc acgcctatga tatggctcta cagcttccat
     1681 ccaacatcct gcgcttaccc aactaattag aggatacgaa cctcttggat catgcctgct
     1741 gtgataggca atgtttgaaa gactatcctc tcctttcaac aggtaatcag gggagcactg
     1801 caaagccttg gctaaggcca ataggttttc gccattgggc tcagtttcag atcgctccca
     1861 ttgggaaata gcaacattag acacgccaac catcttgcca agggcagcct gcctaatctt
     1921 gagttctttt ctgcgagcgc gaatacgctc acccatcagt tgtgtattca tagttaagac
     1981 atcttaaata aacttgactt aagattcctt tggtggatag tttaagtgtt ctttaatttc
     2041 ggagcgagtc tatgtacaaa aaagatgtta ttgaccactt cggaacccag cgtgctgttg
     2101 ctaaagcact aggcattagc gatgcagcag tctctcagtg gaaagaagtt atcccagaga
     2161 aagacgccta tcgattggaa atcgttacag ctggcgccct gaagtatcaa gaaagtgctt
     2221 accgccaagc ggcataagta aattgctctt taacagttct ggcctttcac ctctaaccgg
     2281 gtgagcaaac atcagcggca aatccactgg gtgtgccgct atatatcaat ataggaaaat
     2341 taacaaatgg cacaagcaag ttacagcaag ctaacacagc gagaaattga tcgcgcagaa
     2401 acagatttac t
//
LOCUS       DAZGJW010000349         2399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZGJW010000349 DAZGJW010000000
VERSION     DAZGJW010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.352"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..97
                     /locus_tag="ACHKIK_005312"
     CDS             <1..97
                     /locus_tag="ACHKIK_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO9086248.1"
                     /translation="PLSRESIAVSMIVGRDNCAFDPDRGNFVLKN"
     gene            complement(678..1112)
                     /gene="uspF"
                     /locus_tag="ACHKIK_005313"
     CDS             complement(678..1112)
                     /gene="uspF"
                     /locus_tag="ACHKIK_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO9086249.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(1253..2386)
                     /gene="ompN"
                     /locus_tag="ACHKIK_005314"
     CDS             complement(1253..2386)
                     /gene="ompN"
                     /locus_tag="ACHKIK_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO9086250.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT          664 a          510 c          557 g          668 t
ORIGIN      
        1 tcctttgagc agggaatcaa tcgcagtttc aatgattgta ggaagagata attgtgcttt
       61 tgaccctgac agaggtaact tcgttttaaa aaattaaggt agcaatggcg ggttaacttt
      121 tcgtaaaatg aaattaattc tcggtacggc atgtaaaacg cggcacctcg tatgcaagaa
      181 cgtgccgcgg ttggctggta agcttttcga tagtgcgagt atctaatgat ttccagccgg
      241 aactgattct attggaaaga aatcatgggc gccagattga ttagttatag atttttactg
      301 tggcaaaaat gggacaaaag gccgcaaaag gggcaaaaat ggggcaaaaa aagtgtagat
      361 tattgtgctt tattgtagtt gttaatcctg cctaacatat tgaaaaacat aaaagttatt
      421 gttcgtcaga tagttgcggt ttttactctg acttgttcag gttgtattgt tctttcttac
      481 taatttcttg attttgcgac atttaaaagc gactcaattc gttatatggc atcagaagag
      541 tatgcgtcat gccggaacgc ccagcataag aaatctgata taaaaactgt ggcgtgtatg
      601 gtacggatta gaggggaaaa tgtcagcaca tttgcgaaat gaatcaaaaa gcccgcagca
      661 atgtgcgggc gttagtgtca gcgcacaacc agcacggagc actctgcgtg acgcactaca
      721 gctgcggcgt tagaaccgag cagataagtg gtgatatccg gtcgatggga agcaatgatg
      781 atcatatgag cggggatctt cttcgccaat tccagaatgc ggtcttttgg cgagccttcc
      841 tcaacatgga catgcactct gtcggttggc agtttaaatt ttttaatgat ctcttccagt
      901 tgcgatttgg cttccgcttt caggtcatcc attgccggta attctgcgga atacgctaaa
      961 cccagagagg catagtaggg cagtgaaggt attaccgtca ggaaatgaac ctctgcatca
     1021 tcaatctttg cctctgcctc aacgtggcta atcacgcgtt gagttaattc tgaatcggaa
     1081 atatcgatag ggacaagaat cgttctgttc ataaaacctc ctgttttagt atccgcataa
     1141 agtgtaacgc cagatgacac tttttgtgta atgacggagt tcacattttt aatttagatc
     1201 aaaggaggaa gaataagcag aaaaagcccg ccataacagc gggcaggagg atttagaact
     1261 gataaaccag acctaaagcg acaatatcat cggtagagat gccgttggca gcgtagaaac
     1321 tgtcatcttc atccaacagg ttgattttat agtcaacata agtggacatg tttttattga
     1381 aatagtaagt cgcgccaaca tcggcgtatt taaccagatc tttatcatca acacctgccg
     1441 ggttgtctgc accaccggca gcgtgcaggt cacggccttt agacatcagg aaagagactg
     1501 ccggacgcag accaaaatca aactggtact gtgcagtgac ttcaaagttc tgggttttgt
     1561 ttgcaacagc ataatcgctg tcgccaaacg gggtcatatt acgcgtttct gaatacatgg
     1621 ttgccaggta aatattgtta gcatcgtatt ttagcccagc agtccacgcg tctgctttat
     1681 caccacccgc cgcagtatgg ttaacctggt cattggtgcg gtcagaagag gtgtatgccg
     1741 caccagcgct aaagcccatg cctaaatcat atgttgtgga aagaccccag ccgtcaccgt
     1801 tttcatggcg aacatcacgt ccgttgttgg tgccttcctg accattactg gcaccttcgt
     1861 tgttaccttg atactgcacc gcgaagttca gaccatttac cagaccgaag aaatcagtat
     1921 tacgataagt cgcgacgcca ttggctcgac cagtcataaa gttgtctgca ttggtataag
     1981 agtcaccgcc aaattcaggc agcatatcgg tccagccttc gatgtcgtac attacgccat
     2041 aattacgtcc gtaatcgaaa gaaccgtaat ctgcaaattt cagtccggca aatgccagac
     2101 gggtccatga ctggtttttt gaagattcag tgttgtttgc ctgaatattg tattcccatt
     2161 gaccgtagcc agtaagttga tcgttaattt gggtttcgcc tttaaaaccc agacgcgcat
     2221 agctctggtc gccatctttc gctgaattat cagaaaaata atgcaggcca tcaactttgc
     2281 catacagatc taatttgttg ccgtctttat tataaacttc ggctgcatgt gcagcacctg
     2341 cggcgagcag ggcaggaatt aaaagtgcca gtactttgct tttcattgaa taaatcctt
//
LOCUS       DAZGJW010000350         2399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZGJW010000350 DAZGJW010000000
VERSION     DAZGJW010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.353"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>2399)
                     /locus_tag="ACHKIK_005315"
     CDS             complement(<1..>2399)
                     /locus_tag="ACHKIK_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9086251.1"
                     /translation="LKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQY
                     CDQRVPDGFGGTEPRMTFNAYLSQQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPS
                     DVVWPYTSSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPEAI
                     LRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTGLLETQTVDFTLGSQGLRHTPGDIIEI
                     CDNDYAGTLTGGRILSIDAASRTLTLDREVTLPETGTSTVNLINGSGKPVSVDITAHP
                     APDRIQVSTLPDGVATYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEA
                     IVDNGARFEPMSGSLNSVIPPAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFLLRL
                     TVTADDGSERLVSTARTTETTYRFRQLAPGNYRLTVRAVNAWGQQGDPASVSFRIAAP
                     AAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRITDIRQVETTARYLGTGLYW
                     IAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGRASDDAEGYLDFFKGKITESHLGKE
                     LLEKVDLTEDNTSRLDEFSKEWKDANDKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTE
                     EGKLSQFLVAANRIAFIDPANGNETPMFVAQGNQIFMNDVFLKRLTAPTITSGGSPPA
                     FSLTPDGKLTAKNADISGSVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKA
                     FPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVR
                     K"
BASE COUNT          465 a          759 c          638 g          537 t
ORIGIN      
        1 tttttccgca ctatcagacg gcatgaggag taaatatcag tatgctctct ctcatgctta
       61 gcgccactga atgccaccgc cggaataaca atctgccggt caaacggctg atcgtcataa
      121 accctgacgg taatggtccc tgatggccac cgctccggtg cacgggaatc ccgggggaaa
      181 gctttgccca ctgttttaac gagatcgcct tcaatctggt tcgcggacag ttttcccaga
      241 acccgacagt tctcattaat cgtgacgttg ttgagcgtcc cggagttcgc attcacacta
      301 ccactgatat ccgcattttt agcagtcagc tttccgtccg gtgtcaggga aaatgccgga
      361 ggactgccac cactggtaat ggtgggggcc gtcaggcgct tcaggaacac gtcgttcatg
      421 aatatctggt tgccctgcgc cacaaacatc ggagtttcat tcccgtttgc cgggtcaata
      481 aacgcgatac ggttagcggc aaccagaaac tggctcagct tgccttcctc cgtgtcctcc
      541 atgctgaggc caatacccgc gacataatgt ttgccgtctt tggtctgctc aattttgacg
      601 ccccacatgg cattccattt atcgttagcg tccttccact ctttcgaaaa ctcatccagt
      661 ctgctggtgt tatcctccgt cagatcgact ttttccagca gctccttgcc gagatgagat
      721 tcggttatct tgcctttgaa aaaatccaga taaccttccg catcatcgct cgcccgaccg
      781 acggcctcca cgaatgccga tttgccaaca gtgttcacac tgcggatata aaagtaataa
      841 tcatggcccg gtttgatatt gatactggcg gctatccagt acagccccgt gccaaggtag
      901 cgggctgtgg tttcaacctg cctgatatcg gtaatccgct tttccgagaa ccagaactca
      961 aactgtaccg tcgggtcata aacggcaaga tgcggcgtgg cggttatctg aaaatagccc
     1021 ggcgtcagct caatcctcga cggtgctgcc ggtgcggcaa tccggaacga taccgacgcc
     1081 ggatcaccct gctgccccca cgcatttacc gcccggactg tcagcctgta gttccccggc
     1141 gccagttgcc tgaagcggta tgtggtttcc gtcgtccggg ccgtgctgac cagccgctca
     1201 ctgccgtcat ccgctgttac ggtcagacgg agcaggaagc tcacgccctt caccaccttc
     1261 ggcgtgtccc agcgcgccag cacctgatat tccccgctgt ctgcggtgac ttcggcagtc
     1321 aggtgctgca ctgccggcgg gatgacgctg ttcagtgagc cggacatcgg ctcaaagcgg
     1381 gccccgttat ccacaatggc ttctttttcc ggcacatgct gcaccgccgt gatggcaaag
     1441 gtgccgtccg tgttttcccg gatggaaaca cagcggaaca ggcgacgacg cagtgacggc
     1501 agggagagtc cccacacacc gtatgtcgcc acgccatccg gcagggtact gacctgtatc
     1561 cggtccggcg cggggtgtgc ggtgatgtcc acactcaccg gcttaccgct gccgttaatc
     1621 aggttcaccg tcgatgtacc tgtctccggc agtgtcacct cacggtccag cgtcagggtg
     1681 cggctggcgg catcgatgga caggatacgt ccgccggtca gggtcccggc atagtcgtta
     1741 tcacagattt caataatgtc accgggtgtg tgacgcagcc cctgtgaccc gagcgtgaaa
     1801 tccaccgtct gcgtttccag cagtccggtc tttatcaccc acagcccggc acggtgggcc
     1861 tgaccgcgac tggtgcaacc gaacgcatcc atcttcagca ggttgcgccc gtagcgcagt
     1921 atggcttccg ggtcttccac cagttccgtg gaggtctgcc agccgttctg cgggtcggtg
     1981 taattcacct ccaccgccgt gtggcggtcc ttcagggcgc tgaagctgta gcggaacccc
     2041 acgccgttat catccaccac cacatcgctg ctggtgtacg gccacaccac atccgacgga
     2101 cggtcctgaa cgaacgtcag cgtctggccg ttccataccg gcatacagcg catcgccgag
     2161 cagaaatcac tgagaacgtc ccacgcctta cgctgctgtg acaggtacgc attaaacgtc
     2221 atccgcggct ctgtccctcc gaagccgtcc ggaacacgct ggtcacagta ctgcccgatg
     2281 gcatacagcg cccatttgtc cacgtccgcg gcccccaggc gtttccccat cccgtagcgc
     2341 gggtgggtca gcatgtccca cagacaccag gccgggttgt tgctgtatgc cggtttcag
//
LOCUS       DAZGJW010000351         2362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZGJW010000351 DAZGJW010000000
VERSION     DAZGJW010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.354"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1164
                     /gene="iha"
                     /locus_tag="ACHKIK_005316"
     CDS             <1..1164
                     /gene="iha"
                     /locus_tag="ACHKIK_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO9086252.1"
                     /translation="ENKGRELVRSVLKRDKWGLAGQPRELKESNLILNSLLLTPLGES
                     HLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDEWHLTDALALTAGSRYEHHE
                     QFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLHKGISGVSGQGKTNLLGNPD
                     LKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSYSINDNTNSYVNSGKARLHG
                     VEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSYTPEHMVNAKLNWQITEEVA
                     SWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAWTVVDAGLSWKMTDALTLNA
                     AVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYVIPERNYWMSLNYQF"
     gene            complement(1382..1532)
                     /locus_tag="ACHKIK_005317"
                     /pseudo
     CDS             complement(1382..1532)
                     /locus_tag="ACHKIK_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1744..2064)
                     /locus_tag="ACHKIK_005318"
                     /pseudo
     CDS             complement(1744..2064)
                     /locus_tag="ACHKIK_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
BASE COUNT          622 a          541 c          606 g          593 t
ORIGIN      
        1 gaaaataaag gtcgtgagct tgtacgcagt gtactgaagc gcgacaaatg ggggcttgcc
       61 ggtcagccgc gggagcttaa ggaatcgaac cttatcctga attcattact gcttacccct
      121 ctgggagaat ctcatctggt tacggtgggg ggcgagtttc agagctcgtc catgaaagac
      181 ggagttgtcc ttgccagcac aggtgaaact ttccggcaga aaagctggtc ggtatttgct
      241 gaggatgagt ggcatctcac ggatgcactt gcgctgactg cgggcagccg ctatgaacat
      301 catgagcaat tcgggggaca cttcagtccg cgtgcatatc tggtctggga tgtggcagat
      361 gcctggacgc tgaaaggcgg tgtgaccacg ggatataagg cacccagaat ggggcagcta
      421 cataaaggga ttagtggtgt gtccgggcag ggaaaaacaa atctacttgg taaccccgac
      481 ctgaagccgg aagagagcgt cagttatgag gctggggtgt attacgataa ccccgccggt
      541 ctgaatgcca atgtcacagg ttttatgact gacttctcca acaagattgt ctcttattcc
      601 ataaatgata acaccaatag ctatgtaaac agcggaaagg cccggttgca cggtgtggaa
      661 tttgccggca cattgccgct gtggtcagag gatgtcacgc tgtcactgaa ttacacctgg
      721 acccgaagtg aacaacgtga tggtgataac aaaggtgcgc cgctgagtta tacccctgaa
      781 cacatggtga atgcgaaact gaactggcag atcaccgaag aggtggcatc atggctgggt
      841 gcccgttatc gcgggaaaac accacgtttc acccagaatt attcgtcact gagcgctgta
      901 cagaagaaag tgtatgatga gaaaggagaa tacctgaaag cctggacggt ggtggatgca
      961 ggtctgtcgt ggaagatgac ggatgccctg acgctgaatg ctgcggtgaa taacctgctc
     1021 aacaaggatt acagtgacgt gagcctgtac agtgccggta agagtacgct gtatgccggt
     1081 gattacttcc agacgggatc atcaacaaca ggatatgtga tacctgagcg aaattactgg
     1141 atgtcgctga actatcagtt ctgataataa caaaacgcta tcactgacgg tagaatacgt
     1201 tgccactgca actcctggcg gaacagtggc aacgtattag gttaagtgca tttccgatcc
     1261 gctaatgaga tttcgttacc aacaactaat atcgtcacag gaaatgcacg gattattttt
     1321 aacttatcat ttacatactt gtccagagtg taagcgcacc gtgaaggacg tggggtaaaa
     1381 attagtttac agagagagtg acgttccagg gcaacaactc tttcatgcgg ttggcaggcc
     1441 aggtgttgat tacactgatc acgtggcgta gccacgtttc cggctcgatt ccgttaagtt
     1501 ttgcagctac cgatcaggct gtacatcact gccgcactat cgctcgtcat ctcaaagtcc
     1561 ggtctcgtca gcaggaaggt atcattctct cccgccattt ttccaggggc ccggtcagat
     1621 aagtcccttt gtctatcgct gactcctgac tcataacccg gttagcagaa tgcagggtca
     1681 ccacctcgcc acgaccaaat ccaaataagt caattgcacc ttctcaatcg ccattttgtc
     1741 agtaagcgta cagcctgaac tgatggtatc ttcaccatca atgacaacgg tgatcgcaat
     1801 tttactgacg ttcgccggaa cacgatccag tgctatctca atgctggcct gctgcgaacc
     1861 ggtaacgagc ctgactgccc cctcaggaga agacaaatta ttataaaaga taaagtcaga
     1921 atcgccactg acctttccct gagcattaag catgaacagg caggtatcgg gttcgccttt
     1981 aaagccggat ttggctgggt actgaagatt cagcctgatc gtagattgct gaaggggtat
     2041 gttttgtccg gattgtaaat tcatattaac tctcctgatt tttgattatt attaatgcgc
     2101 agcgtttata tatgttccct aggcttagtt ctggacgctg gatattcggt gaggcgtaaa
     2161 tatggtatga caccattttt cataacgctg aagtttctat acctgttgaa tttgaatttt
     2221 cattgaccgg gtatcttatt ttccagggcc cgttccttca taagtcgcaa aagtaacata
     2281 tatccgaagg gcatgctgtt gatatcagac acggaatact ggctttaacc agcaaccaca
     2341 gataaacccg gggcctgcat aa
//
LOCUS       DAZGJW010000352         2359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZGJW010000352 DAZGJW010000000
VERSION     DAZGJW010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.355"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..587)
                     /locus_tag="ACHKIK_005319"
     CDS             complement(<1..587)
                     /locus_tag="ACHKIK_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO9086253.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVRVEEMQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKN
                     NITQGSGKEWSKQDRDTFDPTHDLDLYCDQASGLV"
     gene            complement(645..992)
                     /locus_tag="ACHKIK_005320"
     CDS             complement(645..992)
                     /locus_tag="ACHKIK_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO9086254.1"
                     /translation="MVTKTITEQRVEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETALTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            complement(1029..>2359)
                     /locus_tag="ACHKIK_005321"
     CDS             complement(1029..>2359)
                     /locus_tag="ACHKIK_005321"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO9086255.1"
                     /translation="EYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTGY
                     DGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTAC
                     SAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGNQ
                     FEALPAEVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLAD
                     ELINASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLAT
                     MLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPEM
                     TVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLAA
                     GSGKAEPVSASTTAMFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
BASE COUNT          466 a          754 c          602 g          537 t
ORIGIN      
        1 ttcacaagac cgctggcctg gtcgcagtag aggtcaagat catgtgtagg atcgaacgtg
       61 tcacgatcct gttttgacca ctccttacca ctaccctgag tgatgttatt cttcgtcgac
      121 ctgccaaaat cgacctcaat tttctcgaac tggtctcctt ccatcgtgta tttgccatac
      181 agcacggcat tcaccgcctg catttcttcc acccggacaa tggcgtgctc ttcctgtttg
      241 aggttatcag tgatgatacg cagacgacgg taggccgggt cgttcagctg agccggatct
      301 tcaccgggaa gacgctcaac cgcctgctgg taattaaatt cgtgtttcgg cttgacgtag
      361 cccggacgta acacgcgggt ttcaccacca cgatgacgca gcacttttcc ttcaacaacc
      421 ggggaaacat aggccgccac cggcgttttt ccggtaattt tgtccagcat cacctcttcg
      481 gtatggaaat tcaccgtacg gcggaaaaac agctccagaa acagcgcacg aaatttcact
      541 ttttgttcgg tataaccgag taactggcgg gtcgtaaaca atcccataaa tcagttcctt
      601 tcattcagaa atcagtcagg ccaccgcggt ggcctgataa cgtgttacgg cagcgccgcg
      661 tgactcaggg cactgccggc aaaggcgttg gcctttttgt gttcatccac actttcaggc
      721 cagcggattg cctccgtcgc aaaggtcccc gacttgtaat aggtcagcgc cgtctctgtg
      781 ccttcaagcg gcagtaccag tatgccaacc gcactaccgg ctttctgtcc gtcccagacc
      841 accagtttcc cggtggcctc atccagcatc aggggcgtca gtgccggtgt tgccgaggaa
      901 atcccgctgc tgcctgtggc ggtatgagcc ggatcattac cggcaaaaat acgtacttcc
      961 acacgctgtt cagtgatagt tttcgtcacc atattgtaaa aacctcctgt tgatggtcag
     1021 cactggcttc atggcatggc catgagcatt ttcacgtccg catcaccgtc tgctgacgtc
     1081 tgtggcacgc caccctgtac cgctgccggt gaatggttcg ccatgatgcg ttcaaacatg
     1141 gcggttgtgg atgcagagac cggttctgcc ttacctgatc ccgcagccag cacagcccgg
     1201 gcgctctcca cagtcattcc cgggcaggca gccagctgtt cagcctgcgc ctcagcccct
     1261 tttgcctcat ccagtgccat gatctgatca cggagtgagg gtccggcatc cgcctgcggt
     1321 gaagcagcca ggatcgggcg ggctttttcc accgtcatct ccggcatcgc cgccagcgtt
     1381 gccgccagtt gttcacgacc tttcgcttct tcacacgcca taatgcgatc ggcttcactc
     1441 tgcgcggatg ccaccggctg ctgtggtgcc gccgcggcca gaatcgcccg ggcctgttca
     1501 acgctcatgc cctgttgtcc tgccagcatc gtggcaagct gttcacgtcc tttcgcttcc
     1561 tggcatgtca ggatccccat cactcgctgg ttctcctgcg cggcggcttc cgttgcagtt
     1621 aattgcggca tagtgcctcc tctgacatta ctgttcagcg ccgtggccat cacactgatg
     1681 gcatccgacg cattgattaa ttcatccgcc agcccggcct caatgccgga ctgaccttca
     1741 aaaacggcgg cctctgttcc cgtgacagca tcaacagaca gaccggtata catcgctact
     1801 ttttcagcaa acatccggcg cgccgcatca atgcgctgtt gcatgtcctg gcgaacctct
     1861 gccggcaacg cttcaaactg attgccatcc accttgtgcg cccctgagta aatcagcgtg
     1921 atatcaacac cggcctgcgc cagatgaccg gcatagctga catggctcat catcacgcca
     1981 atggagccga tacgggatgt ctgggtaacc agccgtcggg agcaggccga cgccagcagc
     2041 atggctgcag agcaggccgt gtcattgcac agtgcccaga ccggcttctg ctgacggagg
     2101 cggtaaatca tgtcagcgca gtcaaacgcg ccggcggcct gcccgcccgg actgtcaatg
     2161 tccagcagta cgccccgcac ctggctatcc gccattgcct gctgaagaca ggcgacaatg
     2221 ccgtcatagc ctgtcattcc ggaaaatggc cgcatacccc ccagccggtg caccagcgtg
     2281 ccggtcaccg gcagtacagc aataccgttc accacccggt aaacacgggc cggtcgttta
     2341 cctccggcca tgtactcgt
//
LOCUS       DAZGJW010000353         2336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZGJW010000353 DAZGJW010000000
VERSION     DAZGJW010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.356"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..132
                     /locus_tag="ACHKIK_005322"
     CDS             <1..132
                     /locus_tag="ACHKIK_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFO9086256.1"
                     /translation="KGIEESKSIKRPLHFMRGVIPTTEMKKYCSENCAEKDQMAHEL"
     gene            206..481
                     /locus_tag="ACHKIK_005323"
     CDS             206..481
                     /locus_tag="ACHKIK_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086257.1"
                     /translation="MITNYEVTVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVTKMSLVHIGKNLPAEKKAGFLATLIAMKLKGEI"
     gene            580..>2336
                     /locus_tag="ACHKIK_005324"
     CDS             580..>2336
                     /locus_tag="ACHKIK_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFO9086258.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTCATLDYLIVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQE
                     DAPETEEPTGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDRKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRSMAEPTTLDNAALLRLTDAIKAVFSITKKHQ
                     PYEFKNFISAWLDTEHIDRGLLTKEWRKGNRVSRITRTASGANAGGGNLTDRGEGFVH
                     DLTSLARDVATGVLARSMDVDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGL
                     DYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMP
                     QRVTEEGKQDDEEKPQPSGAMADEQATAETVEPNATEHHQNTQPLDAQSQVNSVDAKY
                     QELRAELHEARKNIPSKNPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDSVYQN
                     QPETEKISPDAKQPEPVVQQEPEIVCNACGQTGGDNCPDCGAVMGDATYQETFGEENQ
                     VEAKEKDPEEMEGAEHPHN"
BASE COUNT          735 a          552 c          560 g          489 t
ORIGIN      
        1 aaaggaattg aagaaagcaa aagcattaaa agaccacttc atttcatgcg tggagttatc
       61 ccaacgacgg aaatgaaaaa atattgtagt gaaaattgtg ccgaaaaaga ccagatggca
      121 cacgaacttt aattaactga ctatgcgaaa ctgaatttat gccagcaatg gcagggattc
      181 gctcaacctt aattaaggag aaaaaatgat taccaattat gaagtcactg ttgtaactac
      241 cgatgacatt gttcacgagg ttaatctgga aggaaagcgt attggctacg tgattaaaac
      301 agaaaataaa gaaaccccat tcactgtggt tgatattgac ggtccatcag gcaacgtaaa
      361 aacacttgat gaaggtgtca caaaaatgag tctggttcac atcggaaaga atctgcccgc
      421 agaaaaaaaa gccggatttc tggcaactct gattgcaatg aaattaaaag gtgaaatctg
      481 aaaaaagaaa gcctgcacaa cgtgcaggcc tgagtgaaga acctgggaca tttattcatc
      541 actcgcagta attttaatct gagttgaggt taaaaaacaa tgagcacaaa accactcttc
      601 cttttacgga aagcgaaaaa atcatccggt gaacctgacg tcgtcctgtg ggcaagcgac
      661 gattttgaat caacctgtgc cactctggac tacctgatcg ttaagtcagg taaaaaactg
      721 agcaactatt ttaaagctgt tgccacaaat tttcctgtcg ttaatgacct tccccctgaa
      781 ggtgagatcg attttacctg gagtgaacgc tatcaactca gcaaagactc catgacctgg
      841 gaactaaaac cgggagcagc gccagacgac gttcaccatc aggaggatgc accggaaacc
      901 gaagaaccga cgggaggcca ggaagaaaac gcgcaggcag acgcccacga ggattgccag
      961 gattgcgaag tctctgtagc cactttgcgg ttcacacagc gtcttctgca catttttacg
     1021 tatgcagccg gggatcgaaa atacctgcat catgccaccc gtgaacaacg caaacacatt
     1081 actgctcttg agatggatca ggaaaacagc tatgtccaga atctgctgtt ggccatacgc
     1141 agcatggcag aaccgacaac tctggataat gccgccctgc tccgcctgac tgatgcaatt
     1201 aaggcagtgt tctctatcac gaaaaaacat cagccctatg aatttaagaa tttcatttca
     1261 gcctggctgg ataccgaaca cattgatcgc ggtcttctga caaaagaatg gcgaaaaggg
     1321 aatcgtgttt cacgcatcac tcgcacggct tccggtgcta atgctggcgg cgggaacctc
     1381 accgatcgcg gcgaaggttt cgtccacgat ctgacatcac tggcgcgcga tgtagccact
     1441 ggcgtactgg cccgttcaat ggacgtggac atctataacc ttcatccggc acacgctaaa
     1501 cgcattgagg aaattatcgc tgaaaataaa ccaccctttt ctgttttccg cgacaaattc
     1561 atcaccatgc ctggcgggct ggattattcc cgcgccatcg tggttgcgtc cgtgaaagaa
     1621 gcaccaattg ggatcgaggt catccccgca cacgtcactg aatatctgaa caaagtactg
     1681 actgaaaccg atcatgccaa ccctgatccg gaaatcgtgg atattgcctg cggtcgctcc
     1741 tcggccccga tgccgcaacg agtaacagaa gaaggaaaac aggacgatga agaaaaacca
     1801 caaccatctg gcgcaatggc agatgaacag gcaacggctg aaacagtgga accgaatgca
     1861 actgaacatc atcagaacac gcagccgctg gatgctcagt cacaggtaaa ttctgttgat
     1921 gcgaaatatc aggaactgcg ggcagaactc catgaagccc ggaaaaacat tccatcaaaa
     1981 aatcctgtcg atgccgataa attgcttgct gcatcacgtg gtgaatttgt tgacggaatt
     2041 agcgacccga acgatccgaa atgggttaag gggatccaga ctcgcgattc tgtgtaccag
     2101 aaccagccag aaacggaaaa aatcagcccg gatgcgaaac aacctgagcc agtagtgcaa
     2161 caggaaccgg aaatagtctg caatgcctgc ggtcagactg gcggggataa ctgccctgac
     2221 tgtggtgcgg tgatgggcga cgcaacatac caggaaacat tcggtgaaga gaatcaggtt
     2281 gaagctaagg aaaaagatcc ggaggaaatg gaaggcgctg aacatccgca caatga
//
LOCUS       DAZGJW010000354         2243 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZGJW010000354 DAZGJW010000000
VERSION     DAZGJW010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.357"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>2243
                     /gene="agn43"
                     /locus_tag="ACHKIK_005325"
                     /gene_synonym="flu"
     CDS             <1..>2243
                     /gene="agn43"
                     /locus_tag="ACHKIK_005325"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO9086259.1"
                     /translation="TGAFVVASELARARGKRGGVAVALSLAAVTSLPVLAADIVVHPG
                     ETVNGGTLANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQWVQDGGTANKTTVTSG
                     GLQRVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHEGAIATGTVINDKGWQV
                     VKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINKNGRQIVRTEGTANTTV
                     VYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQIVKNGGVAGNTTVNQK
                     GRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVVEGKADNVVLENGGRLD
                     VLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSGAAVSGTRSDGKAFSIG
                     GGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAGTTTLNNGAILTLSGKT
                     VNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLTQKAVNLNEGTLTLNDS
                     TVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIPDNATVQSVVDDLSHAG
                     QIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNADRLVIDGGRATGKTIL
                     NLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRLQAGAFNYSLNRDSDESW
                     YLRSENAYRAEVPLYASMLTQAMDYDRIVAGSRSHQTGVNGENNSVRLSIQGGHLGHD
                     NNGGIARG"
BASE COUNT          547 a          606 c          696 g          394 t
ORIGIN      
        1 acgggcgctt tcgtggttgc ctccgaactg gcccgcgcac ggggtaaacg tggcggtgtg
       61 gcggttgcac tgtctcttgc cgcagtcacg tcactcccgg tgctggctgc tgacatcgtt
      121 gtgcacccgg gagaaaccgt gaacggcgga acactggcaa atcatgacaa ccagattgtc
      181 ttcggtacga ccaacggaat gaccatcagt accgggctgg agtatgggcc ggataacgag
      241 gccaataccg gcgggcaatg ggtacaggat ggcggaacag ccaacaaaac gactgtcacc
      301 agtggtggtc ttcagagagt gaaccccggt ggaagtgtct cagacacggt tatcagtgcc
      361 ggaggcggac agagccttca gggacgggca gtgaacacca cgctgaatgg tggcgaacag
      421 tggatgcatg agggggcgat agccacagga accgtcatta atgataaggg ctggcaggtc
      481 gtcaagcccg gtacagtggc aacggatacc gttgttaata ccggggcgga agggggaccg
      541 gatgcagaaa acggtgatac cgggcagttt gttcgcgggg atgccgtacg cacaaccatc
      601 aataaaaacg gtcgccagat tgtgagaact gaaggaacgg caaataccac tgtggtttat
      661 gccggcggcg accagactgt acatggtcac gcactggata ccacgctgaa tgggggatac
      721 cagtatgtgc acaacggcgg tacagcgtct gacactgttg tgaacagtga cggctggcag
      781 attgtcaaaa acgggggtgt ggccgggaat accaccgtta atcagaaggg cagactgcag
      841 gtggacgccg gtggtacagc cacgaatgtc accctgaagc agggcggcgc actggttacc
      901 agtacggctg caaccgttac cggcataaac cgcctgggag cattctctgt tgtggagggt
      961 aaagctgata atgtcgtact ggaaaatggc ggacgcctgg atgtgctgac cggacacaca
     1021 gccactaata cccgcgtgga tgatggcgga acgctggatg tccgcaacgg tggcaccgcc
     1081 accaccgtat ccatgggaaa tggcggtgta ctgctggccg attccggtgc cgctgtcagt
     1141 ggtacccgga gcgacggaaa ggcattcagt atcggaggcg gtcaggcgga tgccctgatg
     1201 ctggaaaaag gcagttcatt cacgctgaac gccggtgata cggccacgga taccacggta
     1261 aatggcggac tgttcaccgc caggggcggc acactggcgg gcaccaccac gctgaataac
     1321 ggcgccatac ttaccctttc cgggaagacg gtgaacaacg ataccctgac catccgtgaa
     1381 ggcgatgcac tcctgcaggg aggctctctc accggtaacg gcagcgtgga aaaatcagga
     1441 agtggcacac tcactgtcag caacaccaca ctcacccaga aagccgtcaa cctgaatgaa
     1501 ggcacgctga cgctgaacga cagtaccgtc accacggatg tcattgctca gcgcggtaca
     1561 gccctgaagc tgaccggcag cactgtgctg aacggtgcca ttgaccccac gaatgtcact
     1621 ctcgcctccg gtgccacctg gaatatcccc gataacgcca cggtgcagtc ggtggtggat
     1681 gacctcagcc atgccggaca gattcatttc acctccaccc gcacagggaa gttcgtaccg
     1741 gcaaccctga aagtgaaaaa cctgaacgga cagaatggca ccatcagcct gcgtgtacgc
     1801 ccggatatgg cacagaacaa tgctgacaga ctggtcattg acggcggcag ggcaaccgga
     1861 aaaaccatcc tgaacctggt gaacgccggc aacagtgcgt cggggctggc gaccagcggt
     1921 aagggtattc aggtggttga agccattaac ggtgccacca cggaggaagg ggcctttgtc
     1981 caggggaaca ggctgcaggc cggtgccttt aactactccc tcaaccggga cagtgatgag
     2041 agctggtatc tgcgcagtga aaatgcttat cgtgcagaag tccccctgta tgcctccatg
     2101 ctgacacagg caatggacta tgaccggatt gtggcaggct cccgcagcca tcagaccggt
     2161 gtaaatggtg aaaacaacag cgtccgtctc agcattcagg gcggtcatct cggtcacgat
     2221 aacaatggcg gtattgcccg tgg
//
LOCUS       DAZGJW010000355         2240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZGJW010000355 DAZGJW010000000
VERSION     DAZGJW010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.358"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1078
                     /gene="rfaL"
                     /locus_tag="ACHKIK_005326"
     CDS             <1..1078
                     /gene="rfaL"
                     /locus_tag="ACHKIK_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO9086260.1"
                     /translation="LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSP
                     DAGASMKEIFKAIIENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELIT
                     YYKDYQQGIMPFADYRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLIL
                     GTLSRGAWLSVLVIGLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQ
                     TNSSYRYANGTQGSALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHN
                     FALFIWFGTGLLGLVSLMMLYCAILKECIKNSVKNKYRSPYNAYYIILLSFIGYFVIR
                     GNVEQIEPNLLGVYAGLLLAMKNK"
     gene            complement(1114..>2240)
                     /locus_tag="ACHKIK_005327"
     CDS             complement(1114..>2240)
                     /locus_tag="ACHKIK_005327"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFO9086261.1"
                     /translation="FTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIKNPGYSN
                     YTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVHNS
                     MKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIEIV
                     PNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKN
                     LKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLVVI
                     PSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILK
                     TLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          690 a          420 c          390 g          740 t
ORIGIN      
        1 tcttatgaca ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact
       61 atttaaaaca tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact
      121 tcttcaatcc cctgatgcag gtgctagcat gaaggaaatt ttcaaagcta ttattgagaa
      181 tactttacta tgcacaatag caataccgat catattgaga gacgagaaaa gagaagatgt
      241 cgaaaaaatc gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt
      301 gattacctat tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg
      361 tagcatttct gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa
      421 atcagcaaaa taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt
      481 aggaacttta tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct
      541 gatgtttaaa caatggaagt tactattagt aggagtaatg gttgccatca ttgcattgtc
      601 ggttattttc acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa
      661 tagttcttat cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa
      721 tcctgttatt ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt
      781 cattgattat ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt
      841 catctggttt ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat
      901 attgaaagag tgtataaaaa atagcgtcaa gaataaatat cgctcaccat ataatgcata
      961 ttatataatc ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat
     1021 tgaaccaaat ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa
     1081 aataaaaaag gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc
     1141 ttcgaatctt tgagttacgc catcccaact gtagtgttca aacacaaagt cctgaccttg
     1201 ctttgcaact gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga
     1261 gtctgcagtc ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt
     1321 catcccccca cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac
     1381 catgcaaaat ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata
     1441 acaatgcatc ttttcaggcg gtatgcttcc taacataata caacgatcct tcaaacgttt
     1501 tgcaatttcc ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata
     1561 atcaccaatc acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg
     1621 catcaacagc aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc
     1681 ttcaggggag atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc
     1741 taaatcaatt ccatttggta caatctcaat atctgcattt gctaaataag attggtaata
     1801 ctttttcaag tataaactag gcactatcat tttaacattt tgctcaagca attttggttc
     1861 aaatgcatta tgcatatgta taaccactct tgcctgagga gctcgcttac gaatttgagt
     1921 gtatagcttc atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg
     1981 tgcaatattc aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa
     2041 taaccgctta tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa
     2101 ggtgtaattt gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg
     2161 ctgagcaacc tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa
     2221 aataggagta accgtaaata
//
LOCUS       DAZGJW010000356         2207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZGJW010000356 DAZGJW010000000
VERSION     DAZGJW010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.359"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(157..232)
                     /locus_tag="ACHKIK_005328"
     tRNA            complement(157..232)
                     /locus_tag="ACHKIK_005328"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(197..199),aa:Ile,seq:cat)
     gene            complement(372..926)
                     /locus_tag="ACHKIK_005329"
     CDS             complement(372..926)
                     /locus_tag="ACHKIK_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO9086262.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPKLEAFLRDMINGKQKSWLVHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(923..1213)
                     /locus_tag="ACHKIK_005330"
     CDS             complement(923..1213)
                     /locus_tag="ACHKIK_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO9086263.1"
                     /translation="MVNLRKAAKGQICQIRIPGYCNHNPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACNSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKDGYL"
     gene            complement(1213..1812)
                     /locus_tag="ACHKIK_005331"
     CDS             complement(1213..1812)
                     /locus_tag="ACHKIK_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFO9086264.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPIGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
     gene            complement(1884..2096)
                     /locus_tag="ACHKIK_005332"
     CDS             complement(1884..2096)
                     /locus_tag="ACHKIK_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086265.1"
                     /translation="MEKIKKLFSCKYAVIRRDDLSVIVEMDYFPETPKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          551 a          563 c          466 g          627 t
ORIGIN      
        1 aaccactgac cgattgctta gaaggcaatt gctctgtccg gctgagctaa caacgcagaa
       61 taccgataat ggaccgccat cggggacccg cccccgcacc aacaaccctg ttatggtgtc
      121 gtctgctctt cctgataagc taatggcggt ttgtgatggt ggcccttgct ggatttgaac
      181 cagcgacctg gcgattatga gtcgctcgct ctcaccactg agctaaaggg ccgggagcag
      241 aataataacg gtccgtaatt aattccgcaa taaaaaaccc gctcaatggc gggttctggt
      301 aaagttcatg cgtttggttc gcctcgcgat acagctttgc gaagcttacc ggaattgaag
      361 cagtttttac gtcaaaaagc aataactttt ttctctatac caaaagccat aaccattggt
      421 ttgtacaaaa taaattctgc cacctttagc caatgctcaa tgcgtctttc acaggttctt
      481 aaactccatt ccggatgtgc atcattcagc agttcagcca ttttgcgctt agtcatcccc
      541 cgccccacat aacgctgact cagaacattg agcagcccgg gataacctgc cagtacttca
      601 ccaataaccc tgtcgattat taacgcctct gaatcggtac aatgcaccag ccagcttttt
      661 tgcttgccgt tgatcatatc ccgcaaaaaa gcctcaagtt taggtttgtc cagacctgct
      721 tttttcatcc tccggagcgc ctcgttaatt gccgtttttg tcagcttttt agaggtcagc
      781 aactggttga acatatttcc cgtcttaccg ccgccaatat acgaccagcg cccccacata
      841 cgtagtttcc cctgaatcca gacactttcc agcgtggtga gacgaaggtg ctctccgctt
      901 tttcctgtgt tcgttgggta aatcataaat aaccgtcctt tctccagatc tcttgtgtgc
      961 gaaaaacacc ttctgcatgc atcaggcgca attcttcttt ggtgtaatcg ctggttttta
     1021 ctctcccgtc gattaaatcg tggcatgagt tacaggcaat cgctgcctgc atatcgtgtg
     1081 gctttatcgc tgttccgcac gttcccgcca gcctgtaatg cgccagcaca gaggtttcgg
     1141 gattgtgatt gcagtagcca gggattctga tctggcatat ctggccttta gccgctttac
     1201 gtaaattcac cattacgcaa actccagtag ttgtgcggca acattttcga cttcctccgg
     1261 agaggaaaat ttacggaaca ggatccagtt ccacagcaca ttcagtacag atttataaac
     1321 ctgctgaaac tcggtttcgt ccatgttcgc aaatgcgata gattttgccc tgcgcccacg
     1381 actaccatca ggataaatat gctcggtgta aaatccggcc tgaatggtta cccactcgcg
     1441 gaaagcgtca aacgacttta gcaatgccgt atcccgggtt ctgcatgtcg caactgtatt
     1501 aaggtattgc tctgcggcat cactcagggc tggagtgtgt tcccgaccta ctgattcgca
     1561 caggtaatca acgaagcctg ataccagttt tcgttcgcga ggcgtgatcg ccccaccgat
     1621 cggagtccag taatcgaatc ccagttgcag gagtttgaaa aaacgcttgt ggaatgcgta
     1681 gttacgcaca cgcttaaagt ctgcgtgtat ccactcacct atttttattt gatgcaaaaa
     1741 atcgcaactc tccggcgtcg ccgggagaag taatcctgaa gaggtttgtt tgaccagttg
     1801 tatatgcgcc atcgtagttc tccgctggcg cagtagaatg ggtgttcagc ccgttatgta
     1861 gtataccaga attaatgcca atactaacag gatgctctga ctcgcaattc atccagcagt
     1921 ttatcatttc ccataatgtc acttaccctc atcggtaaaa aaattgcctt tcgaccatta
     1981 cgatacatca ttgattttgg ggtttcaggg aagtaatcca tttcgactat aactgacagg
     2041 tcatcacgac gtatgactgc gtatttgcaa ctaaatagtt tctttatttt ttccacgatg
     2101 cctccgagtt tataagtaca aacggttata tccacataga gacaaaaata ttaatctgaa
     2161 aaatatttat ttcacgccgt atatttgatt atttaatgtg caggtac
//
LOCUS       DAZGJW010000357         2130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZGJW010000357 DAZGJW010000000
VERSION     DAZGJW010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.360"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            305..2077
                     /locus_tag="ACHKIK_005333"
     CDS             305..2077
                     /locus_tag="ACHKIK_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086266.1"
                     /translation="MIPNSTSNKGYVCIDMQCSSTSEPTASTSSNRSIKLAASTNVYP
                     ITRNDSELTLNDFLDNSSSTSSLDYINELGSQLTLNDFLDNIKTNEVDRTCTDVVINI
                     PQEIQTNTQENDLLLSDKNNSICIEIDERITKILTCKQKYQLDSIIHEIIPKENESAE
                     TVLHLMRVLNDQYHQVYNQSGCFYKAYMAIHNKIEQILPYAFRAGGGISIHLLIQALF
                     FNGDYNKSPSQSQQPSLYTSPSPTINTEAFLSNVLSLDITQVRILGDLLSATLFHAPT
                     IFYQYPKLIDEVKYCISNKKITGSVIARFTLCLTSTLLTMSPLLMLNGAVKTGSIVRT
                     IGRGVSYVDIPLALAILGDSWYKAYKHGSSDNPNSAQRFISQEAAFKTTQRVLTQGLS
                     LMSSLSGAIMRSLEKGTPPQMMSLFIVNILNLLFHQNPYEGASASANALKRSTYSHNP
                     DILNTQAIALCVDLQHTKNITMPLFKTKDRISYAFNGQRTSPEDQKQILKEVINSCTQ
                     GERAILNTSQSETCKHKIDEIYEKRFSETELNTLPNEMKNFLIFLNKTHEKDISRLSM
                     GNEVNEKIIAVIVKTLAYRESMLC"
BASE COUNT          789 a          395 c          335 g          611 t
ORIGIN      
        1 caccacattc ctgatgtcat actcactaaa caatttttcg acaacatggt agctcataga
       61 acgttatcgt gtagacaccc tctgctacgt attcggtgtt cagtgcaata tctacaaata
      121 ctggaaaaat ctaaaatcga ggcaagtttt tggtgataac tatagttaga ctatattgac
      181 gacctgatgt gctgtatgta ataactaaca aaaaatattt tccatgggat tttttatttt
      241 aatgaaatgc aaaatatttt tatcaatagt tagtattatg gaaaccatta attcaggagg
      301 aaacttgatt ccaaattcaa cttcaaataa aggttatgta tgcattgaca tgcagtgttc
      361 gtcaacctct gaaccaacag cttctacctc cagcaaccgg agtattaaat tagccgcttc
      421 cacaaatgta tatccgataa caagaaacga ctccgagctc actctgaacg attttcttga
      481 taatagctct tctacgtcat cattggacta cattaatgaa ttgggttctc aactgacgtt
      541 aaatgatttt cttgacaaca taaagacaaa tgaggtggat agaacatgta cggatgtggt
      601 aattaatatc ccacaagaga tacaaacaaa tacacaggaa aatgatttgt tattatccga
      661 taaaaataat tcaatatgca ttgaaatcga tgaaagaatt acaaaaatcc taacatgcaa
      721 gcaaaaatat caactggaca gcatcattca tgaaattata ccaaaagaga atgagagtgc
      781 agaaactgtt ctccatctta tgagagttct gaacgatcaa tatcatcagg tatataatca
      841 gtcaggatgt ttttataaag cctatatggc catacacaat aaaatcgaac agatacttcc
      901 atatgcgttc agagccggag gcggaatcag cattcacttg ctcatacagg cattattttt
      961 taatggcgac tataacaaat caccttcaca gtctcaacaa ccatctttat atacatcacc
     1021 ttctccaaca ataaatacag aagcattctt aagtaatgta ttatcactag atataaccca
     1081 ggtacgcata cttggtgatt tactatcagc aactttattt catgcaccaa caatattcta
     1141 tcaatatcct aaactaatag atgaagttaa gtattgtata agtaataaaa aaataacagg
     1201 ttcggttata gcacgattta ctctatgttt aacaagtaca ttactaacca tgtcaccact
     1261 gttaatgctt aatggagcag ttaaaacagg tagcatagta agaactatag gtaggggagt
     1321 gagttatgtt gatataccat tggccttagc tatattaggt gactcgtggt ataaagctta
     1381 taaacatggt tcttctgata acccaaattc tgctcagaga tttatatcgc aagaagcggc
     1441 ctttaaaacc acccagcggg tattaacaca aggattaagt ctgatgtcct ctttatcggg
     1501 agcaatcatg cgctctcttg agaaaggcac accaccacaa atgatgtctt tattcatcgt
     1561 aaacatacta aatctattat ttcatcaaaa tccatatgaa ggggcatcag caagtgctaa
     1621 tgctttgaaa agatcaactt actcccataa tccggacata ctaaatactc aggcaatagc
     1681 tctttgtgtt gacctccagc atacaaaaaa tataaccatg ccacttttca aaacaaaaga
     1741 caggatatct tacgcattca atggacaaag aacatcccca gaagaccaaa aacaaatact
     1801 gaaagaagtt ataaactcct gtacccaagg agaaagagcc attttaaata catcacaatc
     1861 agaaacatgc aaacataaaa tcgatgagat ttatgaaaaa agattctcag aaacagaact
     1921 aaatacatta ccaaacgaaa tgaaaaattt cttgatattt ttaaacaaaa ctcatgaaaa
     1981 agatatttcg cgtttaagca tgggtaacga agttaatgaa aaaataattg cagttatagt
     2041 caagacgtta gcatatagag agtctatgtt gtgttagact ttaactctat attgatataa
     2101 cattagccaa atattcaaca gtgtatgcga
//
LOCUS       DAZGJW010000358         2125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZGJW010000358 DAZGJW010000000
VERSION     DAZGJW010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.361"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            272..1024
                     /locus_tag="ACHKIK_005334"
     CDS             272..1024
                     /locus_tag="ACHKIK_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HFO9086267.1"
                     /translation="MTTVNQIPDANITPSLPPKIREAVDKVKAAKAVWQEERRKQTEA
                     AAMIETIRKRQEDTKTETQALNDEWRNLFRENQGNMTPRMKKLRAEIALGRETLDEFE
                     DLLAAQAAENEFLPWKTADAANHYISEHNRLIETHAVWLWNEFMKEHGQKLIQILGLL
                     KMTLGRSASAVIGVVHTVNDPESVLKQFISEQLTTPALSCNVSSTDDITLPGISIYAD
                     DKAIQDARQSPSPAARSRMLKQRDMVKGGEKK"
     gene            1021..1575
                     /locus_tag="ACHKIK_005335"
     CDS             1021..1575
                     /locus_tag="ACHKIK_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFO9086268.1"
                     /translation="MNAGTITQEALNDYRSAIKSWLTLRNTQSTYQLRLAALLDTEEK
                     PAAYASQLEDLRERLAVLEWQINCAARDGLYAHQIVLESCVTSATENFMSEHGDALTD
                     ALAPFLCAPYGLEAAMKILRTAVARQTEMRTPVISAAYKSIIDETGLTVDASMRADAS
                     ASFTPAQHKVFLARLNRLNEKGVC"
     gene            1577..1849
                     /locus_tag="ACHKIK_005336"
     CDS             1577..1849
                     /locus_tag="ACHKIK_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO9086269.1"
                     /translation="MALKCPECGAVAHARTSAYEAPSVKRSWYQCQNLECSCTFTALE
                     SVDTIIMKPRCNEQESDKAKIPEKQQQTLNRYGSASKLSNRQQIPV"
BASE COUNT          614 a          511 c          515 g          485 t
ORIGIN      
        1 taattttatc tctgtgaaca gtcatgaaca gtagatgatc actttaatta aaacactaca
       61 ccacttaacc acatgtattt actatatttt tattgaagtg aacagtagtg aatagtatgt
      121 attaaaggaa gcgtgtagat cagacatgac acctttctct ggctagccag aacaaggtca
      181 ttgttcagtc actgacacaa tcctcctcgg tagtgcgctt gtatggaccc cggcacaatt
      241 gacacaacaa caaaacacta ccggagcagt catgactact gttaaccaga tccctgatgc
      301 aaacattaca ccctccctcc caccgaaaat tcgcgaagcg gtggataaag ttaaggcagc
      361 aaaagctgtc tggcaggaag aacgacgaaa acaaactgaa gctgctgcaa tgattgaaac
      421 tatccgtaaa cgtcaggaag atacaaaaac ggaaacgcag gcgcttaatg atgaatggcg
      481 aaacctgttt cgtgagaatc agggaaatat gacgccacgc atgaaaaaac tgcgggcaga
      541 aatcgccctc ggacgcgaaa cactggatga gttcgaagat ttgctggcag ctcaggcggc
      601 agaaaatgaa ttcctgccct ggaaaactgc ggatgctgca aaccactaca tcagcgaaca
      661 taatcgcctg attgaaactc atgcagtgtg gctctggaat gagtttatga aggaacacgg
      721 tcagaaactt attcagatcc ttggactgct gaaaatgact ctgggccgaa gcgcttctgc
      781 tgttatcggt gtagttcata ccgtaaacga ccccgaaagt gtgctgaagc aatttatcag
      841 cgagcaactc accactccgg cactgtcctg taacgtatct tcaaccgatg atattaccct
      901 gccggggatc agcatttatg cggacgataa agccatccag gatgccagac aatcccccag
      961 ccctgcagcg cgttcacgga tgcttaaaca gcgtgacatg gttaaagggg gcgagaagaa
     1021 atgaatgccg gaaccattac tcaggaagca ctcaacgatt accgctcggc gataaaaagc
     1081 tggctgacac tacgtaatac gcaaagtacc taccagcttc gtctggcggc tctgctggat
     1141 actgaagaaa aaccggcagc atatgccagc cagcttgagg atcttcgtga gcgtcttgca
     1201 gtcctcgaat ggcagattaa ctgtgctgca cgggatggtc tttatgctca tcagattgtg
     1261 ctggagagtt gtgttacaag cgcaacggaa aacttcatga gcgagcatgg tgatgcactc
     1321 actgacgctc tggctccttt tctttgcgct ccatacgggc ttgaggcggc aatgaaaata
     1381 ttacgaaccg ctgtagcccg acaaacggaa atgcgtactc cggtaatttc agcagcatat
     1441 aagagcatta tcgacgaaac cggattaacg gtggatgcat caatgcgcgc tgatgcttca
     1501 gccagtttca ccccggcaca acataaagtt ttcctggccc gccttaaccg acttaatgaa
     1561 aaaggagtgt gctgatatgg ccctgaagtg tcctgaatgt ggcgcggtcg ctcacgccag
     1621 aaccagcgcc tatgaagctc cgtcggttaa acgctcatgg tatcagtgcc agaatctgga
     1681 atgttcctgc acatttaccg cactggaaag cgtggatacg ataattatga agcctcggtg
     1741 caatgaacag gaatcagaca aagcaaaaat accggaaaaa caacagcaaa ctctcaatcg
     1801 ctatggctcc gcgtcaaagc tgtcaaaccg tcagcaaatt cctgtctgat tagcaaaata
     1861 cgaccacgaa gtcccggtcc agtaccggga ttttttacgc ctttttcctg gctggcctga
     1921 gagcgtatga gtgcatatct atggcgcatg aaaacgcatg agtctcatgc gccatttttg
     1981 acgcgaaagc ccttgtgtgg tggcttctga gacgatttac ggggtgcatg aaaaccagtc
     2041 tgttaagcga agcgggcagg cgggcggggc attgcgcgca ggaatgtgta taccgtttta
     2101 gtaatgatcg ctgacatctc acttt
//
LOCUS       DAZGJW010000359         2088 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZGJW010000359 DAZGJW010000000
VERSION     DAZGJW010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.362"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..510)
                     /locus_tag="ACHKIK_005337"
     CDS             complement(<1..510)
                     /locus_tag="ACHKIK_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO9086270.1"
                     /translation="MRKLYAAILSAAICLAVSGASAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDIRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVSYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTE"
     gene            complement(581..>2088)
                     /locus_tag="ACHKIK_005338"
     CDS             complement(581..>2088)
                     /locus_tag="ACHKIK_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9086271.1"
                     /translation="VLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRF
                     TQLALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVY
                     DPTVQFEFWFSETRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVG
                     KSAFVEGVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKD
                     ANDKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNE
                     TPMFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGRVNANA
                     GTLNNVTVNENCTIKGMLEATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISD
                     DHNFDRQIIIPPIIFNGIAYSDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIP
                     GSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
BASE COUNT          460 a          614 c          489 g          525 t
ORIGIN      
        1 ttcggtcggg ttaaactgca cgccagctcc ccacgccaga gacgtgttgc tgtggcgacc
       61 gtcatcactt ccggtcagca cgtcgtgcgt tttccccttg ttgtcagtta cgcggagata
      121 atccccggag aaagtcgaca cacggctgta agacacaccc gccatcgcat acgcgctgaa
      181 ccattcattc acgcgcacag acggccccgc catcacgctg aaccagcggt tacgcacgga
      241 atcttcatgc cagcggatat cgctgtaacg ggtaagctgg cgattcttgt ctcctgcata
      301 gctgaatgac gtcaccagcc ccagtgtgtc cgtaaactca taacggtatt tcacgttaat
      361 cccgttcaga tcatcgctgc cgggaacgtt cgtcgaggca tgaagatacc ccgcgctcag
      421 cgtggactga tgttcagatg cccatgcaga cgcaccggat acggccagac aaatagctgc
      481 ggacaaaatg gctgcataaa gtttacgcat aattacctct cgcttttctg caataaaaaa
      541 ggcgtcattc ctgacgccct ttattggggt tataaatatt tcaacgaata ctgataccgg
      601 aagccgcttt tttggtcaca atcaccgtac agtcggtgat attccctgcc ccctgattgc
      661 ctttctggaa aatcttaaac tccagagtga cgctaccacc accactaggc atatcaataa
      721 ctgcactgta actaccggga atggcccctt tagtttctct ggatgcgatt aatacaccgt
      781 ttttgcgaac ttcaaaacca taacccgtgt atcttgtacc tcccgggtta ttaccacttc
      841 ccggatcgct atacgctatt ccgttaaaga taatgggcgg aataatgatt tgacggtcaa
      901 agttatgatc atcgctgatg gtgactgtaa ccgtcccgtt tggtgtttcc gtattacccc
      961 acgtaccagc ctgtttcggg aatgatttgg atacagcttt aacgaagtca cctctgacct
     1021 gagtcgcctc cagcatgccc ttaatcgtac agttttcatt taccgtgaca ttgttgagcg
     1081 tcccggcgtt cgcattcact ctgccactga tatccgcatt tttagcggtc agctttccgt
     1141 ctgatgtcag ggaaaatacc ggaggactgc caccgctggt aatggtgggg gctgtcaggc
     1201 gcttcaggaa cacgtcgttc atgaatatct ggttgccctg cgccacaaac atcggcgttt
     1261 cattcccgtt tgccgggtca ataaacgcga tacggttagc ggcaaccaga aactggctca
     1321 gcttgccttc ctccgtgtcc tccatgctga ggccaatacc cgcgacataa tgtttgccgt
     1381 ctttggtctg ctcaattttg acgccccaca tggcattcca tttatcgtta gcgtccttcc
     1441 actctttcga aaactcatcc agtctgctgg cgttatcctc cgtcaaatca actttttcca
     1501 gcagctcttt accgagatgg gattcggtta tcttgccttt gaaaaaatcc aggtaacctt
     1561 ccgcatcatc gctcgcccga ccgacgccct ccacgaatgc cgatttgcca acggtgttca
     1621 cactgcggat ataaaagtaa taatcatggc ccggtttgat attgatactg gcggctatcc
     1681 agtacagcgc cgtaccaaga tagcgggctg tggtttcaac ctgcctgata tcggtaatcc
     1741 gcgtttccga gaaccagaac tcaaactgta ccgtcgggtc ataaacggca agatgcggcg
     1801 tggcggttat ctgaaaatag cccggcgtca gctcaatcct cgacggtgct gccggtgcgg
     1861 caatccggaa cgataccgac gccggatcgc cctgctgccc ccacgcattt accgcccgga
     1921 ctgtcagcct gtagttcccc agcgccagtt gcgtgaagcg gtatgtggtt tccgtcgtcc
     1981 gggccgtgct gaccagccgc tcactgccgt catccgctgt tacggtcaga cggagcagga
     2041 agctcacgcc cttcaccacc ttcggcgtgt cccagcgcgc cagcacct
//
LOCUS       DAZGJW010000360         2082 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZGJW010000360 DAZGJW010000000
VERSION     DAZGJW010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.363"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..286
                     /locus_tag="ACHKIK_005339"
     CDS             <1..286
                     /locus_tag="ACHKIK_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086272.1"
                     /translation="SVAVGDTINLSNYEHIEFSFNSFSKDVCREQNGDIYKIFFRIAS
                     LNNSIVVSENMLTQKNFLEHNTFFSVTGIDESLYEKYIIFITLKAIPPLK"
     gene            307..453
                     /locus_tag="ACHKIK_005340"
                     /pseudo
     CDS             307..453
                     /locus_tag="ACHKIK_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(453..521)
                     /locus_tag="ACHKIK_005341"
                     /pseudo
     CDS             complement(453..521)
                     /locus_tag="ACHKIK_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            741..1954
                     /locus_tag="ACHKIK_005342"
     CDS             join(741..1032,1032..1954)
                     /locus_tag="ACHKIK_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO9086273.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFGEGGV
                     RPPLEKMMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARARRDDWLK
                     KEIQRVYDENHKVYGVRKVWRQLLREGIRVARCTVARLMVVMGLAGVLRGKKVRTTVS
                     RKAITAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSS
                     METTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYD
                     NTMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKA
                     YYASIGNDELAA"
     misc_feature    1022..1138
                     /locus_tag="ACHKIK_005342"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          542 a          484 c          555 g          501 t
ORIGIN      
        1 aagtgtagct gttggagata caataaattt atctaattat gaacatattg aatttagctt
       61 taattctttt agtaaagatg tctgtcgtga acaaaatggt gatatatata aaatattttt
      121 tagaattgcg tctttgaata attcgattgt tgtgagcgaa aatatgctca ctcaaaaaaa
      181 cttcctggaa cacaatacat ttttcagtgt taccggcata gatgagtctc tctatgaaaa
      241 gtatataatc tttatcactc ttaaggcaat ccctcccctg aagtagcgct gatttccggt
      301 atgcggaatt cgttgtggag aataacaaaa atggtcatct ggagtttaca ggtggccatt
      361 cgtgggacag tatccctgac agcctacaaa acgcaattga agaacgcgag gcatcgtctt
      421 aacgaggcac cgtggcgtcg gttcaaccct taatctagtc cttcccgatc ggttgaacta
      481 ccggatgcct tgtcggtaaa gatgcggtca gcttttaccc cttatccgtg aaggtgccaa
      541 aggcagcgag ctgatttggt tacgtcaacc gcccggcccg ccaaacccca agcatatgct
      601 ggtgtcaacg acggatgaaa agtgatccac ttatatctcc accaacggcc caatattgat
      661 ccaccgtttt actcaggatt agcttctgaa ccgccccggg aatcctggag actaaacttc
      721 ctgagaaaga ggtaaacagg atgactaaaa atactcgttt ttcccccgaa gtccgtcaac
      781 gggcagtccg tatggttctg gaaagtcagg gcgaatatga ctcacaatgg gcgacaattt
      841 gttccattgc tccaaagatt ggctgtacgc cggagactct gcgtgtctgg gttcgccagc
      901 atgagcggga taccgggggc ggtgatggag ggctcaccac cgctgaacgt cagcgtctga
      961 aagagctgga gcgtgaaaat cgtgaactgc gccgcagtaa cgatatcctt cgccaggctt
     1021 ccgcttattt tgcgaaggcg gagttcgacc gcctctggaa aaaatgatgc cactgctgga
     1081 taagctgcgt gagcagtacg gggtcggacc gctatgcagc gaactgcata ttgccccgtc
     1141 aacgtattac cactgtcagc aacagcgaca tcatccggat aaacgcagtg cccgtgcgcg
     1201 gcgcgatgac tggctgaaga aagagataca gcgcgtatac gatgaaaatc acaaggtata
     1261 cggtgtgcgt aaagtctggc gtcagttgtt acgggaaggt atcagagtgg ccagatgcac
     1321 tgtggcacgt ctcatggtgg ttatgggact tgccggtgtt ctccggggta aaaaggtccg
     1381 cactaccgtc agccggaaag ccattaccgc aggcgaccgc gtaaaccgtc agttcgtggc
     1441 agaacgacct gaccagctgt gggtggctga ttttacttac gtcagcacat ggcagggctt
     1501 cgtctatgtg gcgttcatca ttgatgtgtt tgccggatac atcgtggggt ggcgggtctc
     1561 atcgtccatg gaaacgacat tcgtgctgga tgcactggag caggcgttat gggcccgtcg
     1621 accgtccggc acggtccatc acagtgataa aggttctcag tatgtatcgc tggcctacac
     1681 acagcggctt aaggaagccg gattactggc atcaacagga agtacaggcg actcgtatga
     1741 caacacgatg gcggagagca tcaatggtct ttacaaagcg gaggtaatac accgtaagag
     1801 ctggaaaaac cgtgcagaag tggaactggc cacactcacg tgggtggact ggtataacaa
     1861 tcgacgattg ctggaaaggc tgggccatac tcctccggca gaagcagaaa aagcttatta
     1921 tgcttccatc ggaaacgatg agctggcagc ctgagttcac agataaaaca ctctacagga
     1981 aacccggggc ggttcaccgt gatactgtgg ctagagaaag tggttcgtga taggtcgatg
     2041 cgattattgt ttcactgctg ctggcctcct gcatttctgt ag
//
LOCUS       DAZGJW010000361         2081 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZGJW010000361 DAZGJW010000000
VERSION     DAZGJW010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.364"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..276
                     /locus_tag="ACHKIK_005343"
     CDS             <1..276
                     /locus_tag="ACHKIK_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO9086274.1"
                     /translation="VWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGAVVSFKGYYGD
                     LAIVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK"
     gene            328..711
                     /gene="gpFI"
                     /locus_tag="ACHKIK_005344"
     CDS             328..711
                     /gene="gpFI"
                     /locus_tag="ACHKIK_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO9086275.1"
                     /translation="MATKEENQKHLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMPGDESGPEQNHTDDGEQLHNTDATDDVKAVRVRKCLHVMGYCPETGRPVELT
                     YRGMRVLVPSPLATAMIQHGTAEHA"
     gene            704..1057
                     /locus_tag="ACHKIK_005345"
     CDS             704..1057
                     /locus_tag="ACHKIK_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO9086276.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGSGVRIEGSCPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            1072..1605
                     /locus_tag="ACHKIK_005346"
     CDS             1072..1605
                     /locus_tag="ACHKIK_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9086277.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMPGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            1602..1997
                     /locus_tag="ACHKIK_005347"
     CDS             1602..1997
                     /locus_tag="ACHKIK_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO9086278.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDSWMESRIYPAMSAIPALAGMITTM
                     VQQGYDYRRDDDMALWSSADLTYSITYEM"
     gene            2005..>2081
                     /locus_tag="ACHKIK_005348"
     CDS             2005..>2081
                     /locus_tag="ACHKIK_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9086279.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYTGKGDA"
BASE COUNT          461 a          483 c          666 g          471 t
ORIGIN      
        1 gtctggcgtc tgctgaatgg ttttaagctg ttccgcgaaa aactggatac ccgtcgcggc
       61 tcaaattcac aactcgaaac ggcagtgaaa gacctggggg cggtggtgtc tttcaaaggg
      121 tattacggcg atctggccat tgtggtggcc tcttcccgtt atccgaaaca ctggctgacg
      181 gtaggggatc ccgcccgtga atttaccatg acgcagtccg cgccgctgat ggtgttgccg
      241 gacccggatg agtttgtggt ggtgcaggtg aaataatccg tgagcggggg cgaaatgccc
      301 ccgtgtcttt tttcacaggg ggctgatatg gcaacaaaag aagaaaatca gaaacatctt
      361 cgtcaactgg ctggcctgct ggggcgcgag gcggatatgt cggggagtgc tgcggatatt
      421 gcgcaacgtg tgtctgagtg ggaagaggag cttgctgctt cccgggaggg cattatgcct
      481 ggtgatgaga gcgggcctga gcaaaatcac acagacgatg gtgagcagtt gcacaacact
      541 gatgctacgg atgatgttaa agcggtccgt gtgcggaaat gcctgcatgt gatggggtat
      601 tgcccggaga caggccgtcc cgttgaactg acgtaccggg gcatgcgtgt tctggtgcca
      661 tcaccactgg cgacagccat gatacagcac ggaacggctg agcatgcgtg attttcagaa
      721 tgcctttgat gctgcccttg ccggggtgga cagcacgatt gttgaagtga tggggctctg
      781 tgcgcagttc acctcggggg cacagtgtgg cagcgaagtt cagggggttt ttgacgatcc
      841 ggagtcgctg gggtttgccg gtagcggggt ccgtattgaa ggaagctgcc cgtcattatt
      901 tgtacggacg gatacggttc gtgctgtgcg gcgtggtgac acgctgacca ttaatggtga
      961 gacattctgg gtggatcgtg tttctccgga tgacgggggc agttgttatc tctggctcaa
     1021 ccgtgggcaa ccaccggcag ttaaccggcg acgataaacg cagggtgaat tatggcgata
     1081 aaagggcttg atcaggcgat tgaaaatctg agccgggttc gtaaaaacgc cattccggcg
     1141 gcttcagcaa tggccattaa ccgcgtggcc acaacggcga ttaatcagtc ttcgtcacag
     1201 gttgcccggg agaccagggt gagccggaaa ctggtaaagg aacggtccag actgaaacgg
     1261 gccacggtca gaaatccgaa tgccagaatt atcgttaacc gcggtgatct cccggtgatt
     1321 aagctgggga tcaggatgcc ggggcgtcgt ccggacagca tactcaaagc cggtcagcat
     1381 cggtatcagc gggcatttat tcagcgatta aaaaatggtc gctggcatgt catgcagcgt
     1441 gtggccggga aaaaccgtta ccccattgat gtggtgaaaa tcccgatggc ggccccactg
     1501 aaacaggcgt ttgatgagaa tgttgaccgt atccggcgtg aacgcctgcc cggagaactg
     1561 gcatatgcgc tgaaacaaca actgaggatt gcgataaaac gatgaaacat actgatatcc
     1621 gtgctgcagt gctggatgca ctggagctgc atgaacacgg ggcgacgctg tttgatggtc
     1681 gccccgttgt ttttgacgaa gaggattttc ccgcggtcgc ggtttatctg acggatgcag
     1741 agtataccgg tgaagagctg gatgcggata cctggcgggc cacactgcat attgaagttt
     1801 tcctgcctgc tcaggtgccg gattcagagc tggattcgtg gatggagtcc cggatttatc
     1861 cggcgatgtc cgcgatccct gcactggcag ggatgattac cacgatggtt cagcagggct
     1921 atgactatcg tcgtgatgac gatatggcgt tatggagctc tgcagatttg acttattcca
     1981 ttacatacga gatgtgagga cgatatgcca acaccaaatc ccctggcgcc ggtaaaaggt
     2041 gccggtacca ctctgtgggt ttacaccggc aagggtgatg c
//
LOCUS       DAZGJW010000362         2039 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZGJW010000362 DAZGJW010000000
VERSION     DAZGJW010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.365"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..147
                     /locus_tag="ACHKIK_005349"
     CDS             <1..147
                     /locus_tag="ACHKIK_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9086280.1"
                     /translation="GDRKGACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACW
                     GIDR"
     gene            418..987
                     /locus_tag="ACHKIK_005350"
     CDS             418..987
                     /locus_tag="ACHKIK_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO9086281.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMMIDFVLADTGKGKITKAD"
     gene            987..1133
                     /locus_tag="ACHKIK_005351"
     CDS             987..1133
                     /locus_tag="ACHKIK_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086282.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFLVMLVAIFFSTAWRVLTLS
                     GLVG"
     gene            1361..1567
                     /locus_tag="ACHKIK_005352"
     CDS             1361..1567
                     /locus_tag="ACHKIK_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO9086283.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            1632..1856
                     /locus_tag="ACHKIK_005353"
     CDS             1632..1856
                     /locus_tag="ACHKIK_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086284.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
BASE COUNT          527 a          450 c          559 g          503 t
ORIGIN      
        1 ggagatcgaa aaggtgcctg cgaagctatt cgctggtgga ttaaggacgg tggcagggac
       61 tgccgtattc gctcaaataa ctgttacggt caggtatccc gtcgtgacca ggagagcgcg
      121 ctggcgtgct ggggaatcga cagataagca gaatattttg ctgaaaaatg aggtttgctt
      181 acatggatgg ataacacgaa atcctgcaaa ttggcaaaat gtaagtgaat aaagtcaaaa
      241 cagttgttta acactcaggc accgtaatga tgcctttgtc atttctgcgc atctcacgcg
      301 catctcacaa cacagaacct ttcaggatga cccttgagga taccggtttg gctgtcggtg
      361 cctttctgtg ggctggattc ctgtgagaca aggttcatca ctaaaaggaa ataaccgatg
      421 aatatgatgg ccgtgccgtt tcacggcaac tctctttatg tagttaacca taatggcgaa
      481 ccatacgttc ccatgaaacc tgtcgttgcg gggatggggc tggcctggca atcacagttg
      541 gctaagttaa gacagcgttt tgcgtcaact ataacggaaa tcgttatggt tgctgaggat
      601 gggaaacaac gcaatatggt gtccatgcca cttcgaaaac ttgccggctg gctacaaacc
      661 attaatccca acaaagtaaa acccgaaatc cgcgataagg tcatccggta tcaggaagag
      721 tgcgacgatg ttctttacga gtactggacg aagggttttg tcgttaatcc ccgtaaaatg
      781 agcgtgatgg aagaactcaa ccaggcttgt gctgacatga aacgggataa aaacattgcc
      841 agtgtgtttg ctaccgggct gaatgagtgg aaacaggtta aagccgcgca tgtatcaaaa
      901 atccgtacgc tggtaaatga agcgaatatg atgattgatt ttgtcctggc tgatacaggc
      961 aaagggaaaa taacaaaggc ggattgatgg ggtggctaat gatatcagat aaactcataa
     1021 cgctggtgaa gagcctctgt gtacttgtcg gcatttcatt tttagtcatg ctggttgcca
     1081 ttttcttttc caccgcctgg cgagtcctga cgttatcggg actggtgggg tgaaagagag
     1141 atgaaccgtg ttctgtgtgt ggtgattatt gtcatggcgg ttggctgtgg tgcgctgtgg
     1201 ctggcaacaa accattaccg tgacaacgcg ctcacctaca aagcgcagcg cgataaaaaa
     1261 gccagagagc tggaacaggc gaatgccacc attactgaca tgcaggtgcg ccagcgtgat
     1321 gttgctgcgc tcgatgcaaa atactcgagg gaattagccg atgcgagagc tgaaaatgaa
     1381 actctgcgtg ctgatgttgc cgctggtcgt aagcgcctgc ggatcaacgc cacctgcccc
     1441 ggtaccgtgc gtgaagccac cggcacctcc ggcgtgggca atgatgccgc cgtcgaactc
     1501 tctccggttg ctggacgaaa cgttctcggt atcagagacg gaatcatcag cgaccaggca
     1561 gcattgagaa tgcttcagga atatatccgc actcagtgta ttaactagta tttttgttat
     1621 tcggagaatg catgaagaaa ttattggtaa ccgtaaagcc ttttcaggga acaattccgt
     1681 tccgtatttt gcagcgtggt cgtgttcttg ttgaaggttc gttcagtggt aaatgtacgc
     1741 aattacactc caggaccttt caggtgaatg ccacgaatga agagctaacc gttgagtgta
     1801 cgatgaatgc cgctaaatgc cgcatggtat ccgctgcatt acagccagtg tgttgagcga
     1861 ccttattatt catgcgcggt attgtcgccg tattcctgca ttaacagaga ccgcagcccg
     1921 accgggagaa tcctctgcgc gagtgtgcgg ggataatcaa aaacgataca caccggggtt
     1981 taccgcgtta acggagcgcg gcgttgtccc ctcatggtcg ctggtccggt gcgatggtg
//
LOCUS       DAZGJW010000363         2036 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZGJW010000363 DAZGJW010000000
VERSION     DAZGJW010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.366"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(329..826)
                     /locus_tag="ACHKIK_005354"
     CDS             complement(329..826)
                     /locus_tag="ACHKIK_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFO9086285.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     KKHQM"
     gene            complement(823..1356)
                     /locus_tag="ACHKIK_005355"
     CDS             complement(823..1356)
                     /locus_tag="ACHKIK_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9086286.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVESNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            complement(1353..1664)
                     /locus_tag="ACHKIK_005356"
     CDS             complement(1353..1664)
                     /locus_tag="ACHKIK_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO9086287.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     I"
     gene            complement(1669..1884)
                     /gene="essD"
                     /locus_tag="ACHKIK_005357"
     CDS             complement(1669..1884)
                     /gene="essD"
                     /locus_tag="ACHKIK_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO9086288.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
BASE COUNT          564 a          492 c          395 g          585 t
ORIGIN      
        1 agaatggtcc gaaaaaaaat atcagagaga aaaaccaaca aggaaatctc aagaggtaca
       61 aataataaaa ttataacaat gactgcttca gataaatttg taacaaacca gaacaccatt
      121 aacgcatgat taaccaccca tagcaaggat tacttttgta aagaaaaaca cagcaatgaa
      181 agaatagctt tatttattaa taaaacgtgt cattctgatt aagacctttt atcttaccct
      241 taagatttca ggaattttgg ctcatggaag agtccttttt atttaaattt tacattccgc
      301 gatgtaaatg ttccgattta atattaccct acatttgatg ctttttatct cttaaagatt
      361 catagatctg ttgacaagtc actcctgcga tgtagcgttc gtcagcaatt tcagcataaa
      421 gctgagcttc tgctgcaata tctccgagca tgttggtgag cattccttcg gcggttttgg
      481 ttgttttgcc tctgacggca gcggcaagat ctgcggtatg cttcgctgcg tcaaggcgta
      541 tggcatattt ttttgcttcg gcacgcaact ggttaacact atcagacaga taagcagccc
      601 tggcagaaat ttcagcagat ttctgttgtg catctttaac agcctcatca cgggctatag
      661 ttcgcccctg ttcaattatt cgagcagcaa attgagcatt tacctcttgt gataatgcgg
      721 cagcatcacg ttccgcccat tttttttgcc atcctctgtc gctccagaca tttccgacga
      781 taaatcctga caacacgaga aaaatcacca tgaatatctg attcactgtt ctatccccca
      841 gcaggttaat gcgctctcct ggtcacgacg aataacctga ccgtaacagt tatttgaacg
      901 aatgcggcaa tcgcgtccgc catccttaat ccaccagcga atcgcttcgc atgcaccttt
      961 acgatcacca gcattcagcc gcttataaaa cgtcgacggg aaacacttac cggggccaat
     1021 gttataggga caaaatgacg cgatacccgc tttttgtggt tcggtcagtg gtactttaat
     1081 attgctctcc acccatgcca gcgccttatc acgctcaatg gcgttgacct ggtcgcattt
     1141 ttccttcgac agtttcatat tgggaaaaac ggtttttcca tccaccactg tggcaccccg
     1201 acagatggtc catatgccag aaccatcgcg gtatgccatt gtgtggttac cttctttttc
     1261 gtccagaaac tggtcaagta tctgaggagc agatgcgcca gcaccaatca gcgccagaac
     1321 ggcagccgac aggccgtatc tgatttttgt gttcatatat atttatgatg aggacgctcg
     1381 tgcttattgg caggattttc aatcttaaag gagtactgat gctgcagata agactcaact
     1441 ttttctgaca atttttctgc tacttccagg aagacttgcc ggacgctcct tctggctgct
     1501 gcctcataaa actccagcgc agctccttca acacggtcca tggcgacatc caggtcaaaa
     1561 atttcaccgt caaagcgttc cttgtcctgt aaggctacag ttaccgtaac tttattctca
     1621 aaattacgga ctcctttcac aaccagttca tagtcttgag tcattggatt actctcctct
     1681 cgcagcctta cgcctgtctt ctttaatctt gaaataaaga tttgtcagat acgtcagcag
     1741 gccaaaaacc aggctaccca gcacaccgat tgcagcccac tgtgacggag ttactttatc
     1801 gagtaactgt aatgcccaga aaccagcatt acccgccgat gtgccatagg caacacctgt
     1861 tgttaactta tccattgatt tcatatcctc accccgatgt acacggatgg tgcaatatgt
     1921 ttgaaaaaga tcggagtcta cggggtagtt ttgacagcac acgttgttct caacggcgct
     1981 aaagaaacat acacattaaa aatgtgagta attattttga aagaaagtca tatata
//
LOCUS       DAZGJW010000364         2036 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZGJW010000364 DAZGJW010000000
VERSION     DAZGJW010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.367"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..753
                     /locus_tag="ACHKIK_005358"
     CDS             <1..753
                     /locus_tag="ACHKIK_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO9086289.1"
                     /translation="FIMLFKFKIWSDTFLGVALWTFIWAICATIFTFSFGFILALALA
                     KKNIRFAKVWRLIFILPYAIPTFVTLLIFRLLLNGIGPVNSFLNSIGMNSVDFLSVPL
                     NAKITVIAVCVWVGAPYFMLLIAGALTNISRDLYEASEVDGATKWQQFKEITLPMVLH
                     QVAPTLVMTFAYNFNNFGAIYLLTEGGPTNPEYRFAGHTDIFITWIYKLTLNFQQYHI
                     ASVISIIIFIFLSVIAIWQFRRMKSFKDDVGM"
     gene            756..1589
                     /locus_tag="ACHKIK_005359"
     CDS             756..1589
                     /locus_tag="ACHKIK_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFO9086290.1"
                     /translation="MDKFLRKISTLLVYLFLICNSILVLGPVIWTIMASFKKGNNLFS
                     STFSGIEFTFDHYITLFTDTPYMQWYLNTFILATANMLISLFVVTMTAYVFSRYRFRG
                     KRNTLMSILVLQMFPAFLSMTAIYILLSKANLIDTYTGLLLVYVTGSLPFMTWLVKGY
                     FDAIPTSLDEAAKIDGAGHLTIFIQIILPLAKPILVFVGLVLFTGPWMDFILPTLVLR
                     SEDKMTLAIGIFSWIASNSAENYTLFAAGALLVAVPITLLFVFTQKHITTGLVSGAVK
                     E"
     gene            1609..>2036
                     /locus_tag="ACHKIK_005360"
     CDS             1609..>2036
                     /locus_tag="ACHKIK_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HFO9086291.1"
                     /translation="MITVSSLKHSAKSEDSYAYDNETLHVRLRTLRGEVDKVILWIGD
                     PYNWAEGGLDGGNMAGTEAFGWIGGNEIEMEQEAVTEFHDHWFAVFKPQKRRCRYGFI
                     LFGKEGEKFLFGEKRCVDISSPDCEERELSRLNNFFCFPYL"
BASE COUNT          549 a          348 c          435 g          704 t
ORIGIN      
        1 ttcattatgt tgtttaaatt taaaatatgg tctgatacgt ttctaggtgt cgctctctgg
       61 acatttattt gggcaatttg cgcgacgatt tttaccttca gttttggctt tattctggct
      121 ttagcactgg ctaaaaaaaa tatacgtttc gcaaaagtct ggcgattgat atttatactg
      181 ccatatgcca tacccacatt tgtaacgtta cttattttcc gtcttctttt aaatggaatt
      241 ggtccggtta actcgtttct gaacagtatc ggaatgaatt cagttgattt tctgtcggtt
      301 ccattaaatg caaaaataac agtaattgcc gtttgtgttt gggtaggggc accgtatttt
      361 atgctattga tcgcgggtgc attaacaaac atatcccgag atttgtatga ggctagtgag
      421 gtcgatggcg caactaaatg gcaacagttt aaggaaatta cattgccaat ggtgttacat
      481 caggttgcgc caactctggt aatgacattt gcatataatt tcaataattt tggtgcaatt
      541 tatcttctga ctgaaggtgg tccgactaat ccggaataca gattcgctgg gcataccgat
      601 atttttatta cctggattta taaactcaca ttgaatttcc agcaatatca tatagcttct
      661 gtaatctcca tcataatatt tatcttctta tcggttatag caatatggca attcagacgg
      721 atgaaatcat ttaaagatga tgtagggatg tgacaatgga taaattttta cgtaaaatat
      781 caacgctctt agtttatctt tttttaatct gcaactccat tcttgtgctt gggccagtga
      841 tatggacgat aatggcatct tttaaaaaag ggaataatct atttagttca acatttagtg
      901 gcatcgagtt tacatttgac cactacataa cgttgttcac ggatacgcca tatatgcagt
      961 ggtatttgaa cactttcatt cttgctaccg ctaacatgtt aatttcactg ttcgttgtga
     1021 cgatgaccgc ctacgttttt tcaaggtatc gatttcgtgg aaagcgtaat actttaatga
     1081 gtattcttgt attgcaaatg tttcctgcat ttttatcaat gactgcaatt tatatcctgc
     1141 tatcaaaggc caatttgata gacacatata ctgggttgct tctggtttat gtaaccggtt
     1201 cattgccctt catgacgtgg ttggtaaaag gttattttga tgctattcca acttctttgg
     1261 atgaggccgc taaaatagat ggagcagggc atctgactat ttttattcag ataatattgc
     1321 cattggctaa gccgattctc gttttcgttg gattagtttt gttcactgga ccatggatgg
     1381 acttcatctt gccaacgttg gtgcttcgta gtgaggataa aatgaccctg gcgattggaa
     1441 tttttagttg gattgcttct aattcagcag agaattatac gctttttgct gctggggcgc
     1501 ttttagttgc agtacctatc acattgttat ttgtattcac acagaagcat ataactactg
     1561 gacttgttag cggtgccgtt aaggaataat ttttagggat tagtatttat gattacagtt
     1621 agttcgttaa aacactcagc aaaaagtgaa gatagctatg cctatgataa cgaaactttg
     1681 catgtacggt tacgcacatt aagaggagaa gttgataaag ttattctgtg gataggcgat
     1741 ccatataatt gggctgaagg tggtcttgac ggcggtaaca tggccgggac agaagcgttc
     1801 ggctggattg gcggcaacga gatcgagatg gaacaagaag ctgtcaccga atttcatgat
     1861 cactggtttg cagtgttcaa acctcaaaaa cgccgttgcc gttacggttt tatacttttt
     1921 gggaaagagg gagagaagtt tctttttggt gagaaaaggt gtgttgatat cagttcaccg
     1981 gactgcgaag aacgagagtt aagccgctta aataactttt tttgcttccc ttatct
//
LOCUS       DAZGJW010000365         1995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZGJW010000365 DAZGJW010000000
VERSION     DAZGJW010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.368"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..100)
                     /gene="yqcG"
                     /locus_tag="ACHKIK_005361"
     CDS             complement(<1..100)
                     /gene="yqcG"
                     /locus_tag="ACHKIK_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HFO9086292.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNES"
     gene            239..910
                     /gene="queE"
                     /locus_tag="ACHKIK_005362"
     CDS             239..910
                     /gene="queE"
                     /locus_tag="ACHKIK_005362"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO9086293.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     repeat_region   1250..1827
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1315..1339
                     /rpt_unit_seq="tccccgcgccagcggggataaaccg"
BASE COUNT          522 a          456 c          474 g          543 t
ORIGIN      
        1 tagattcgtt atcattaaaa actaacacag aaaaaataaa tataattttg gtgaatgttt
       61 tgacatgatt aaatccattt tctttatttt cttctgacat aatacgttac tccctttaat
      121 tattaacgat atgtttcgca tttttcttta aatctgcctg taacagaatg ttaataagac
      181 atgattgttg tggcaggatc tgcaataatt aacaattatc cacttcacag agaatgctat
      241 gcagtacccg attaacgaga tgttccagac cctgcaaggt gagggttact ttaccggcgt
      301 ccccgccatt tttattcgtt tacagggatg cccggttggc tgtgcctggt gcgacaccaa
      361 acacacctgg gaaaagcttg aggatcggga agtctccctt ttcagcattc tggcgaagac
      421 caaagagagt gataagtggg gggcggcgag cagtgaagat ttgctggctg tcattggtcg
      481 gcagggatat accgcgcggc atgtggtgat tacgggtggt gagccttgca ttcatgattt
      541 gctgccactg actgacctgc tcgaaaagaa cggttttagc tgccagatcg aaaccagtgg
      601 aactcatgaa gtacgctgca caccgaatac ctgggttacc gtatcgccaa agctgaacat
      661 gcgcggcggc tatgacgtgt tgtcacaggc actggagcga gccaacgaaa tcaagcatcc
      721 agtggggcgc gtacgtgata ttgaagcact ggatgaacta ctggcaacgc tgaccgatga
      781 taaaccgcga gtcattgcac tgcagccgat tagccaaaag gatgatgcca cacgtttgtg
      841 cattgaaacc tgcattgcgc gtaattggcg tttgtcgatg caaacacata aatatctaaa
      901 tattgcctga ttaaacattt ataagcgtta taaatgggtg gaacctgtaa agacttctac
      961 tcatttatat tgtttgtcgc ctctgaaaac tcctccattt tacccatcca gggctaatca
     1021 ttagcattct ctacaaattc tgtggcatta atttttcgct ggagtgaaaa ttattgcggt
     1081 aaagtttggt agattttagt ttgtatagag ttattttaaa tatttacctt tttaatcaat
     1141 ggattaagtg ctctttaaca taatggatgt gttgtttgtg tgatactata aagttggtag
     1201 attgtgactg gcttaaaaaa tcattaatta ataataggtt atgtttagag tgttccccgc
     1261 gccagcgggg ataaaccgat ctcctcatcc tcccagctac cgacagtagt gtgttccccg
     1321 cgccagcggg gataaaccgc aagatctgcc gcgagtggtt tcacccggca ggtgttcccc
     1381 gcgccagcgg ggataaaccg gatatttggg cgtaataccg cccggatagt gtgtgttccc
     1441 cgcgccagcg gggataaacc gtggtacgga acatgctgtc tatatttcga ccagtgttcc
     1501 ccgcgccagc ggggataaac cgccagtgga gccgccgcga atttacacac gattgtgttc
     1561 cccgcgccag cggggataaa ccgtaaggcc gtcgccggat cagcctggct atgccgagct
     1621 ccccgcgcca gcggggataa accgttcttg cgggtgttgc aaatattctt cacgtagagc
     1681 tccccgcgcc agcggggata aaccggagcc tgacgagact actgaggccg ttctgtcgag
     1741 ttccccgcgc cagcggggat aaaccggacg ccgccgccgc gaagccgttt ccgatgttga
     1801 gctccccgcg ccagcgggga taaaccacca tataacccgt tatctctttc tcaagttttt
     1861 atattagcag tacttgtaat aagcaacata tccacgtaac acctcatgtt caaaatagtt
     1921 ctccatgcca gagaggttca caattatcga tacaaaaaat taaatttaat caaagtgtta
     1981 tttgtatgat tctta
//
LOCUS       DAZGJW010000366         1978 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZGJW010000366 DAZGJW010000000
VERSION     DAZGJW010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.369"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..537
                     /gene="escR"
                     /locus_tag="ACHKIK_005363"
     CDS             <1..537
                     /gene="escR"
                     /locus_tag="ACHKIK_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFO9086294.1"
                     /translation="VLGILKNALGIQQVPPNMALTSVSLILTMFIMSPIILQINDNIS
                     QEPINYTDSDFFQKVDEKILSPYRGFLEKNTEKENVEFFERAAQKKLGNETILKKDSL
                     FILLPAFTMGQLEAAFKIGFLLYLPFIAIDLIISNILLALGMMMVSPVTISIPFKILL
                     FILVGGWQKLFEFLLVVN"
     gene            537..806
                     /gene="escS"
                     /locus_tag="ACHKIK_005364"
     CDS             537..806
                     /gene="escS"
                     /locus_tag="ACHKIK_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFO9086295.1"
                     /translation="METGYFVQLCVQTLWIIFILSLPTVIAASVVGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            806..1582
                     /gene="escT"
                     /locus_tag="ACHKIK_005365"
     CDS             806..1582
                     /gene="escT"
                     /locus_tag="ACHKIK_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFO9086296.1"
                     /translation="MNEIMTVIVSSFFCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIVLKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFITVIFVIHGGIQSVLDKLYLSYEILPLQAD
                     IAFNRALVDFLFSLWDSFIKLMLSFSIPMIIGIFLCDMGFGFLNKTAPQLNVFTLSLP
                     VKSLIAIFILLLVIHVFPDFITANIHSDIIDRSLPSMINE"
     gene            1575..>1978
                     /gene="escU"
                     /locus_tag="ACHKIK_005366"
     CDS             1575..>1978
                     /gene="escU"
                     /locus_tag="ACHKIK_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFO9086297.1"
                     /translation="MSEKTEKPTPKKLRDLKKKGDVTKSEEVMAAVQSLILFSFFSLY
                     GASFFVEVVELVNTTIDSLNRPFLYAIREILGAVLNIFLLYILPISLIVFVGTVTTGV
                     SQTGFIFAVEKIKPSAQKISVKNNLKNIFSVK"
BASE COUNT          596 a          314 c          358 g          710 t
ORIGIN      
        1 gtattgggga ttctgaagaa cgcattaggt attcaacagg taccaccaaa tatggcgcta
       61 acatcagtat ctttgatact gacaatgttt attatgtctc cgataatatt acagataaat
      121 gataatattt ctcaggaacc aatcaattac accgactctg atttttttca aaaagttgat
      181 gaaaaaatat tatcaccata ccgcggtttt ttagaaaaaa atacagagaa agaaaatgtt
      241 gagttttttg aacgtgcagc tcaaaaaaaa ttgggtaatg aaactatctt aaaaaaagac
      301 tctctattta tactgttacc agctttcacg atggggcagc ttgaagctgc attcaagata
      361 ggttttttgc tctatttacc ttttattgcg atagatttga tcatttccaa tatcttattg
      421 gccttgggta tgatgatggt atcgccagta acaatttcga ttccttttaa gatcttgtta
      481 ttcattcttg ttggaggctg gcaaaaatta ttcgaatttc tgttggtggt gaattgatgg
      541 aaactggata ttttgttcaa ttatgtgtgc aaacgctttg gataatattt atcctctcac
      601 tgcctacagt catagcggcc tctgttgtcg gcattattat tagcctggtt caggctataa
      661 cgcagttaca ggatcaaaca ttgccttttt tgctaaaaat aatagcagtg tttgctacgc
      721 ttgccctgac ttatcactgg atgggaacaa caatcatcaa cttctcgtcg ataatatttg
      781 aaatgattcc gaaggtgaat ggctaatgaa tgaaataatg acggtcatag tatcatcatt
      841 tttttgtata ctcaggccgc tggggatgtt tattattcta ccaatatttt caacaggtgt
      901 tttgctcagt aactttatac gcaactcaat tatgattgcg tttacacttc caattatagt
      961 agagaactat acattttcgg aaaaattacc atccggaatt tttcagttga caggcatagt
     1021 gctaaaagaa ataagtatag gctttttcat tgggttatca tttactattc ttttttgggc
     1081 aatagatgcg gctgggcaga ttattgatac tttaagaggt tcaacaatat cttcaatttt
     1141 taacccgtct ataagtgatt catcttctat cactggtgtt attttatacc aatttatcac
     1201 tgtgatcttt gttattcatg gtgggataca aagcgttctg gataagttgt atttatccta
     1261 cgagatatta ccattacaag ccgatattgc attcaatcgt gctttagtag attttttatt
     1321 ttcgctatgg gattcattta ttaaactgat gttatcattt tcaattccca tgattatcgg
     1381 tatcttctta tgtgatatgg ggtttgggtt tctcaataaa acagcgcctc agctaaacgt
     1441 attcacatta tcactgccgg taaaaagttt gatagcaatc tttatattgt tattagttat
     1501 tcatgttttc cctgatttta ttacggcaaa tattcattct gacataattg acagatcgtt
     1561 accgagcatg attaatgagt gaaaaaacag aaaagcccac acccaaaaaa ctaagggatc
     1621 taaaaaagaa gggcgatgta acaaaaagtg aagaggtaat ggctgcagtg cagtcattaa
     1681 tcttattttc atttttttct ttatatggtg cgagtttttt cgttgaggtt gttgagttag
     1741 ttaatacgac tatagactcg ctaaatagac cgtttttgta tgccattcgt gaaatattag
     1801 gtgcggtatt aaacatattt ttattgtata ttttgccaat ttctttgatt gtctttgttg
     1861 gaactgttac aactggtgta tcacaaacag gattcatctt tgcggttgaa aaaataaaac
     1921 catcggctca gaagattagt gtaaaaaata acctgaaaaa tattttttct gtaaagag
//
LOCUS       DAZGJW010000367         1976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZGJW010000367 DAZGJW010000000
VERSION     DAZGJW010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.370"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..979
                     /gene="cnf3"
                     /locus_tag="ACHKIK_005367"
     CDS             <1..979
                     /gene="cnf3"
                     /locus_tag="ACHKIK_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024231387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytotoxic necrotizing factor CNF3"
                     /protein_id="HFO9086298.1"
                     /translation="VVDRRFIFPEPPTPPKLSLIQKISQRFFTEDIDETSRINFQRLN
                     SGNINVLKGRGSLSSKNQRSIYLRFDAVNADDLRPDEIYVKKDQFDDLGYDRYFYNNA
                     VGLDGSPTLNTYTGEFLTDPSLFGSLYWSKYNLTNKTSIIRVANSARGANGIRIALKE
                     VQENKPVIITNGNLSGCTTIVARKGEYLYEVHTGTLEPLLGFTSTTGVKKAVEVLSTL
                     AEQEIPSLAGTINNDFLVDFLAENFDKSLVTYSSSTLKPDSIITISRDNVSTFPYYTD
                     DIIHPGFGTSVTILVRIDDNTVVKSLSESYVTNADGSRISVFKVLSKDF"
     gene            1314..1868
                     /locus_tag="ACHKIK_005368"
                     /pseudo
     CDS             1314..1868
                     /locus_tag="ACHKIK_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          617 a          380 c          445 g          534 t
ORIGIN      
        1 ggtggtcgac cgtagattta tatttcctga acctccgacc ccaccaaaat tatcacttat
       61 acaaaaaata tcacaaagat tctttacaga agatatagat gaaacatcca gaattaattt
      121 tcagagatta aacagcggta atattaatgt actaaaaggc agggggagtt tatcatcgaa
      181 aaatcaacgt tcaatttacc tgcgttttga tgccgttaat gctgatgatc tccgaccaga
      241 tgagatctac gtcaaaaaag accagtttga tgacttgggt tatgatcggt atttctataa
      301 taacgctgtg ggattagatg gctctcccac actgaacact tatactggtg aatttctgac
      361 agacccatct ttattcggtt cactttactg gagtaaatat aatctgacca ataaaacaag
      421 cattattcgt gtagcaaatt cagccagggg ggcaaacggt atcagaatag cgcttaaaga
      481 agttcaggaa aataaacctg taatcatcac gaatggaaat ctaagtggtt gtaccacgat
      541 cgtcgcacga aagggagagt atctgtatga agtgcatact ggcacattag agcctctgtt
      601 aggttttacc agtacaacag gagtaaaaaa agcagtggaa gttttgtcga cactcgcaga
      661 acaagaaatc ccttcactcg cgggaacaat aaataatgat tttctggtag attttttagc
      721 agaaaacttt gataaatcat tagtaacgta ttcatcatca acactaaaac cagacagcat
      781 aattaccatt agtcgtgata atgtatctac ttttccttac tatactgacg atattatcca
      841 tccaggattt ggcacatcgg taactatatt ggtgagaata gatgataata ctgttgtaaa
      901 gtctctgtca gaaagttatg ttacaaatgc agatggctcc aggatatcag tatttaaggt
      961 attatcaaaa gatttttgat taagaagcat tattctactc ttttgtgctg tttgtcagac
     1021 aagagggtga aaataggctg acctcaacaa gcactgcttt ttctggtagg tgagtcgcta
     1081 acgcattggg tagcagccca atagcgttag cggcatgtta atctctcgcg ttttagtaag
     1141 tcacaacaca taattgggtt tgatagccaa tgaatgagaa cgtacgccag tatcataagt
     1201 atatgatatg gcttaaaaaa tataatgtac attgaacaat gtcatcaaag cagtatcatt
     1261 tttattgaca attggcattt aacggacaga gttgtctata aatcaattta gttgcgtgct
     1321 gggcccatgc ccggcgaaaa atccacgatg tatacatcag cagcaaaagt acgatggcag
     1381 aagaagccct gaagcgcatc agtgaactgt acgccatcga ggatgaaata cggggcttac
     1441 cggagtctga acgtcttgca gtcaggcagc agcgaagcaa atcgttactg acgtcgctgc
     1501 atgaatggat ggtggagaag aatggcacgc tgtcgaaaaa atccagactg ggcgaagcgt
     1561 tcagctatgt actgaatcag tgggacgccc tctgttatta cagtgatgac ggtctggcgg
     1621 aggcggacaa taacacagca gaaagagcgc ttcgtgccgt ctgtctcggg aaaaagaatt
     1681 acatgttctt cggcaacgat catggtggcg agcgtggtgc gctactgtac gggctgatcg
     1741 gcacctgccg actgaacggt atcgatccgg aagcgtatct gcgccatatc ctgagcgtgc
     1801 tgccggaatg gccttccaac cgagttgatg aactcctgcc atggaacgta gtactcacca
     1861 ataaataagt gtcaatacgg cgctccgtta acgcttactt atttacaagt tgaaaggatg
     1921 gtatatgttg aaaatgcatg gattttccag atgttttacc agtaagaagc aggtgg
//
LOCUS       DAZGJW010000368         1955 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZGJW010000368 DAZGJW010000000
VERSION     DAZGJW010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.371"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            108..794
                     /locus_tag="ACHKIK_005369"
     CDS             108..794
                     /locus_tag="ACHKIK_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO9086299.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(885..1021)
                     /locus_tag="ACHKIK_005370"
     ncRNA           complement(885..1021)
                     /ncRNA_class="other"
                     /locus_tag="ACHKIK_005370"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(1065..1201)
                     /locus_tag="ACHKIK_005371"
     ncRNA           complement(1065..1201)
                     /ncRNA_class="other"
                     /locus_tag="ACHKIK_005371"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            1420..>1955
                     /locus_tag="ACHKIK_005372"
     CDS             1420..>1955
                     /locus_tag="ACHKIK_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9086300.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWG"
BASE COUNT          513 a          424 c          498 g          520 t
ORIGIN      
        1 acatgatact aactatatat tgatcgttgc attgttggca tgtaacacgc tgcagcgagt
       61 aacattggta atgaaatata atcaacatgg ataggtgaaa attaattatg agctttatgg
      121 cttttgtaac ttgtttgaca attgcattta tcgccggttt tgctgttgcc agaaaattga
      181 cactccgaaa agttgcctca tcggtaatca tcgctaaatc gcaacccgtc ccttcatctg
      241 caacttcccc tgccgctaag gcgtcgacaa ggagctatat accgcagaaa acctatcgtg
      301 actctcgttg gcaagagcga gatgagttat ttcagggaat ggttgccgaa ggtattttat
      361 tggaaagaaa agagtggtta atcacgcgtt gtgaaaaaat gtatttcgat aatctggaaa
      421 aatggtttgg acattattgt catatccatt gccaggtctc tctggggcgc ttgatcaact
      481 ttccagaaca acctgagttt agcgaagaag aaagaaagcg tttctttgca atttataatg
      541 gcatggcaat ggattatgtt ctggtttcaa agaagaacaa taaaatagtc tgtgttattg
      601 agctggatga cccgagtcat gaaagggcgg atcgcattat tcgtgaccgt tggttggata
      661 caattatgac tcttgcacgg gtgccttttg tacgagcttc tgtcactaaa ttggaggaaa
      721 ttcctccggt ctgggagact cgtgacaaaa ttcgcccatt agtcgcggcg acaaagagtg
      781 aaccggtgca ttaactaagc atcccggtcg gggcttctca tccccccggt gtgtgcaata
      841 tacgaaaaaa agcccgtacc ttcgtacgag ctcttcttta aagatggcgg tgaggggggg
      901 attgactcgc ttcgctcgcc ctgcgggcag cccgctcact gcgttcacgg tctgtccaac
      961 tggctgtcgc cagttgtcga accccggtcg gggcttctca tccccccggt gtgtgcaata
     1021 tacgaaaaaa agcccgtact ttcgtacgag ctcttcttta aatatggcgg tgaggggggg
     1081 attgactcgc ttcgctcgcc ctgcgggcag cccgctcact gcgttcacgg tctgtccaac
     1141 tggctgttgc cagttgtcga accccggtcg gggcttctca tccccccggt gtgtgaaata
     1201 tacgaaaaaa aagcccgtac tttcgtacga gctcttcttt aaatatggcg gtgaggggga
     1261 ccgcctgaaa taaatctgac ttaatccagg ggtgttatat aatttcatta taaacaatgt
     1321 attacacttt ttgttgtccg atattatcta atgtattctt agtgaagcga gagactaatg
     1381 gcatactgga tggcatacca gagtgacggg aattagcgta tggcagtatt gacggatacg
     1441 aaagcaagac atatcaaacc tgatgacaaa ccattgcccc atgggggaat taccggactg
     1501 acccttcatc cttcttcagt aaaggggcgg gggaaatggg tttttcgtta tgtaagtccg
     1561 gtgacacaaa aaagacgtaa tgctggattg ggaacttacc cagaggtcag tattgctgaa
     1621 gctgcacgta ctgcccggat aatgcgagag caacttgctg caggtgatga tcctctggag
     1681 attaaaaagg ctgaatctga gaaagtcgct atcccaacat ttgccgatgc agccaggcgt
     1741 gtacatgcag aactgtctcc tggatgggaa aatccaaagc atgtaaggca gtggttatcg
     1801 acgcttgaga attacgcgtt tcctcaactg ggagcaaaaa cgctggattc gattacggct
     1861 gcggacgtgg cagaaacact gcgtccagtc tggttaacct tgtcagaaac ggcaagccgg
     1921 gttaaacagc gcattcatgt tgttatgcag tgggg
//
LOCUS       DAZGJW010000369         1912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZGJW010000369 DAZGJW010000000
VERSION     DAZGJW010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.372"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1912
                     /locus_tag="ACHKIK_005373"
     CDS             <1..>1912
                     /locus_tag="ACHKIK_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HFO9086301.1"
                     /translation="GGISSASLEGFISSGLEVCASRIGGTAGRYLSNVAKVIKLPLLD
                     IGINIWSLYDSSLNHARATTQIEYVSTAIDVSFSSINTALSIGAIAYPPLAIAIVPIT
                     IFSHEVKNYAVYVNQINERHKLWLEAEKYLDNGSAKVLSINKDTGIIDLSNNQVLGNV
                     HLDMRENPPILHGEKSYNSGKNIGSHPDMTDREIMESGAYNFACTKLSDAGEPDIFGW
                     GDKDVCNSMELSESQLANGYSNRQWPSQSPVIPEGIYNTVYLGYGETLRANTEVTLSS
                     TGYFYEIARAYTDDELSEPLLTVCNQRSSVIGGKEPLTIIIPALEHGMLGSNLHMIER
                     FKNYNFSISGGKGGIKLMVGGIGDYNIECTPGVRNIISFEQLSRDFNLDLDLSDERKQ
                     NFNFHHPSGFYSGKVMSITQKGINSIVGTKLGYDKIRGNNLDNTFSLGNGGGVIYSGG
                     GSNTYFVPATLQDNLHIYISEKSNGNHIILGETHTRLSIECHFSDDKREFIKMDLYNG
                     GDVILESDTLQKINTFSKNITIQTADGVMANWDDESSTLSVYSIDMIAWRDKNKTEKE
                     PLPLDVIQLTNWRIHNSCSLFYDKYQVDIEANKLTYTILFPDTELPVQLNYASVIYGN
                     HGAKYTFLDSGSK"
BASE COUNT          652 a          283 c          382 g          595 t
ORIGIN      
        1 ggtggtatat ctagtgcctc gcttgaagga tttatatctt ctggtttaga agtgtgcgcg
       61 tcaagaatag gtggcaccgc tgggcgttat cttagcaatg tggcaaaagt aataaaatta
      121 cctttgctag atataggcat aaatatttgg tcattatatg actcttcatt aaatcatgcc
      181 agagccacca cacaaattga atatgtatca acagcgatag atgtatcttt ttcatctata
      241 aatactgctt tatcaatagg cgcaatcgca tatcctcctt tggcaatagc tatagttccg
      301 ataactattt tttctcatga agtaaaaaac tatgctgttt atgtgaatca aattaatgag
      361 cgacataagt tatggcttga agctgaaaaa tatctggaca atggaagcgc aaaggttcta
      421 agcattaaca aagatacagg cataatcgat ttatcaaata atcaggtgtt aggtaatgtt
      481 catttagata tgagagaaaa ccctccgata ttacatggtg aaaaatcata taatagcggg
      541 aagaatattg gaagtcatcc agatatgact gacagagaaa ttatggagtc tggagcatac
      601 aattttgcat gtacaaaact ctcagatgca ggtgaacccg atattttcgg ttggggagat
      661 aaggatgtat gtaattcaat ggaactgtct gaatctcaat tagctaatgg gtattctaac
      721 cgccaatggc cttcacaaag tccagttatt cctgaaggga tttataatac agtatatctt
      781 gggtatggag aaacattgcg agctaataca gaagttacat tgagtagcac tggttacttt
      841 tacgagattg caagggcata tactgatgat gaactatctg agccattatt aacagtatgt
      901 aaccaacgta gtagtgtaat tggtggaaaa gaaccattaa caataatcat tccagctctt
      961 gaacatggta tgcttggctc taatctacat atgattgaac gttttaaaaa ctacaatttt
     1021 tcaatatcag gagggaaggg cgggataaaa ttaatggttg gtggtattgg tgattacaac
     1081 atagaatgta cccccggtgt tagaaatatt atttcttttg aacaattatc acgtgacttc
     1141 aatctggatt tggatttatc agatgagagg aaacaaaatt ttaactttca tcatccctct
     1201 ggtttttatt caggaaaagt catgagcata acgcaaaagg gcataaattc gattgttggt
     1261 actaaactgg gatatgataa gattcgtggt aataatttag ataatacctt ttccctcgga
     1321 aatggggggg gggttatata ttctggagga ggaagcaata catattttgt tcctgctaca
     1381 ctacaggata atttgcatat atatatatca gaaaaatcaa atggtaacca tattatttta
     1441 ggtgagacac acactcgctt atctattgaa tgccacttta gtgatgataa aagagagttt
     1501 ataaagatgg acttatataa tggaggtgat gtaattttag aaagtgacac tttacaaaaa
     1561 ataaatacat tttcaaaaaa tataacaata cagacagcgg atggagttat ggcaaactgg
     1621 gatgatgaat caagtactct gtctgtttat tcaatagata tgattgcctg gagggataaa
     1681 aataaaactg aaaaagagcc tttaccactt gatgtgattc agttaactaa ttggaggata
     1741 cataactcat gctctttatt ttatgataaa tatcaagttg acattgaagc caataaatta
     1801 acgtatacaa ttttgtttcc tgatacggag ttacctgttc aacttaatta tgctagtgtt
     1861 atttatggta atcatggggc aaaatataca ttcctcgact caggaagtaa ag
//
LOCUS       DAZGJW010000370         1904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZGJW010000370 DAZGJW010000000
VERSION     DAZGJW010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.373"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..1904)
                     /locus_tag="ACHKIK_005374"
                     /pseudo
     CDS             complement(1..1904)
                     /locus_tag="ACHKIK_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          399 a          605 c          457 g          443 t
ORIGIN      
        1 ctgaccgctg ctctgccgga ccaccacaga gaacaaccgt tcacccgtca tactgatgtc
       61 aatatccgcc accccgtcaa ccaggcattc ccatccccgc atcccgtgcg ttttttcata
      121 cgcccgccag agtccgccct ggtgtgtggc atacgtgccc cggggaaagg atttttgatc
      181 gtcaatggcg gggagtattt ccagtgccgt ggcatcacgc ccgtcctgcg gagccggcag
      241 ggcactcacc gcatccagaa ccgccttctg cagaacatcc ggatcgtagt ctcgaccatc
      301 acgcggaaca ggaatctggc ttacagcctc cttcaccatc tgctcaatca tcggacgcac
      361 atcatcggga gtaatacttt taccgtctgc cggctgcgga atatttgcga ccgcatcatt
      421 caccgccttc tgcagaacat cgggatcata gtcacgaccg tcgcgcggaa caggaatatg
      481 gcgtacagcc tccttcacca tctgctcaat catcggacgc acatcatcgc ccgtcacgca
      541 cttctgtaat accacagaca gggaagccag tttttcttca aacgtttgtg cctgcgcggc
      601 tattttcccc tcaaatgtgc gctgtaaatc cgccagcact gcggaaaatt cttcacccag
      661 cgcacggata atggacagtt cacgttcatt cattttttca gaatccccct gaacatcgcc
      721 ttcaccgcat catgctctgt ttcagtgatt gccttattac cgtcagaggc aacgaccgcc
      781 tgaggcacgg aagctgtttt accggacgac gcgaacggat cttcacgggc atcacggcgg
      841 gacaacgctt ccagactgta gttctgctgc tgaagataca gtgcatcacc gccggccagg
      901 ggcggcaggt tctcccgttt acgggcctca ttgggcgtga gaagcgtatt tttcaccgca
      961 tcccccagcg ttttcatgcg ccgctcactg tccattctca gcagcgtggt gacatcaaat
     1021 tccgtactct cgttttcccc tgtttccagc gcctcatcca gtaacagttc aatggactca
     1081 atcagcgtct gcaggcactg ggaataatac tgctgctcca gcgcctccac gttgtcactg
     1141 gaaggcggtt gtcccacgcc aatcttgtag gccgggacac ggaacaccga acagacaatt
     1201 tcagcggtca tcttcagttg ttccaccgtc tgcgcatcca ccggtgaaaa cgtcgtgggg
     1261 ttgtattttg ccccgttgct cagaatggcc gttttccccg cattttcgcc tgtatacccg
     1321 ctgtcccagt tgctcttcag ttttttcgca ttttcttccg ttatactgcc ggggatctca
     1381 atcaccccgg acggcctgcc gccatttctg aaaaaagacg tcgaattttc ctgaatatga
     1441 tgcccctgcg tggccgccag tcctgcggca tacaccggcg gcaaccctat aagcggatga
     1501 aaaaaacagt taaaccggtc gtggatcact tcccgggcag gcaccgtcac cgcctcagtg
     1561 atcccgcagt tccggtccgg cgtgatgcgg tagaacacct cgccgtcatc cgccaccaga
     1621 ggttcaaccc ggttccagtc cagaatacgc agctctttga tctgcccccg ggcattacgg
     1681 attttcagca ccaccgtatt gccgtgacgc agtttggcgt tcagccacag ttcaaaaaac
     1741 tggatgcggt tctgctgggc gttgggacga cgacagaggc gggcaatatc cccccggcgc
     1801 gtttccctgc gtatcccctg cgcatccgtc tgcataagac gcagccgcat tttggcgata
     1861 tcctgggata tcagcgaaat acatgcaaac accgcatgaa agga
//
LOCUS       DAZGJW010000371         1892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZGJW010000371 DAZGJW010000000
VERSION     DAZGJW010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.374"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..182
                     /locus_tag="ACHKIK_005375"
     CDS             <1..182
                     /locus_tag="ACHKIK_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9086302.1"
                     /translation="TPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTP
                     ALLQTLIREIKGSSH"
     gene            182..529
                     /gene="tnpB"
                     /locus_tag="ACHKIK_005376"
     CDS             182..529
                     /gene="tnpB"
                     /locus_tag="ACHKIK_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9086303.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            549..>1892
                     /locus_tag="ACHKIK_005377"
     CDS             549..>1892
                     /locus_tag="ACHKIK_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="HFO9086304.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIA"
BASE COUNT          462 a          527 c          534 g          369 t
ORIGIN      
        1 taacgccgga gccggataat aaaatcccgg cccccgcaca ggaaccagag cagataaata
       61 caccgtccga cagtctgtgt tgtgagctgg ttctgccggc cggaactctc aggcttaaag
      121 gtaaactgac gccggcgtta ttacagacac ttatccgcga aataaaaggg agcagccact
      181 gatgatatct ctccctgcag gttcgcgtat ctggctggtt gcaggtatca ccgatatgcg
      241 aaatggcttt aacggcctgg catcaaaagt tcagaacgtc ctgaaggatg acccgttctc
      301 cggacacctg ttcatcttcc gcggacgccg gggtgaccag ataaaagtgt tgtgggctga
      361 cagtgacgga ctgtgcctct tcaccaaacg cctggagcgg ggccgcttcg tctggccagt
      421 cacccgtgac ggcaaggtgc accttactcc ggctcagtta tccatgcttc ttgaaggtat
      481 caactggaag cacccgaaac gaacggaacg cgctggaatc cgcatataac ccgttgtaaa
      541 gtgaggatat ggacacctca cttgctcatg agaacgcccg cctgcgggca ctgttgcaga
      601 cgcaacagga caccatccgc cagatggctg aatacaaccg cctgctctca cagcgggtgg
      661 cggcttatgc ttccgaaatc aaccggctga aggcgctggt tgcgaaactg caacgtatgc
      721 agttcggtaa aagctcagaa aaacttcgtg caaaaaccga acggcagata caggaagcac
      781 aggagcgaat cagcgcactt caggaagaaa tggcggaaac gctgggtgag caatatgacc
      841 cggtactgcc atccgccctg cgccagtctt cagcccgtaa accgttaccg gcctcacttc
      901 cccgtgaaac ccgggttatc cggccggaag aggaatgctg tcctgcctgt ggtggtgaac
      961 tcagttctct gggatgtgat gtgtcagagc aactggagct tatcagcagc gcctttaagg
     1021 ttatcgaaac acaacgtccg aaacaggcct gttgccggtg cgaccatatc gtgcaggcac
     1081 cagtaccttc aaaacccatt gcacgcagtt atgccggagc ggggcttctg gcccatgttg
     1141 tcaccgggaa atatgcagac catctgccgt tataccgcca gtcagaaata taccgtcgtc
     1201 agggagtgga gctgagccgt gccacactgg ggcgctggac aggtgctgtt gctgaactgc
     1261 tggagccgct gtatgacgtc ctgcgccagt atgtgctgat gcccggtaaa gtccatgctg
     1321 atgatatccc cgtcccggtc caggagccgg gcagcggtaa aacccggaca gcccggctgt
     1381 gggtctacgt ccgtgatgac cgtaacgccg gttcacagat gcccccggcg gtctggttcg
     1441 cgtacagtcc ggaccggaaa ggtatccatc cacaaaatca cctggccggt tacagcggtg
     1501 tgcttcaggc cgatgcttac ggtggttacc gggcgttata cgaatccggc agaataacgg
     1561 aagccgcgtg tatggctcat gcccggagaa aaatccacga tgtgcatgca agagcgccca
     1621 cctacatcac cacggaagcc ctgcagcgta tcggtgaact gtatgccatc gaggcagagg
     1681 tccggggctg ttcagcagaa cagcgtctgg cggcaagaaa agccagagcc gcgccactga
     1741 tgcagtcact gtatgactgg atacagcaac agatgaaaac actgtcgcgt cactcagata
     1801 cggcaaaagc gttcgcatac ctgctgaaac agtgggatgc actgaacgtg tactgcagta
     1861 atggctgggt ggaaatcgac aacaacatcg ca
//
LOCUS       DAZGJW010000372         1892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZGJW010000372 DAZGJW010000000
VERSION     DAZGJW010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.375"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..281)
                     /locus_tag="ACHKIK_005378"
     CDS             complement(<1..281)
                     /locus_tag="ACHKIK_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO9086305.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKASAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAAS"
     gene            complement(291..1610)
                     /locus_tag="ACHKIK_005379"
     CDS             complement(291..1610)
                     /locus_tag="ACHKIK_005379"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO9086306.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAM
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(1591..>1892)
                     /locus_tag="ACHKIK_005380"
     CDS             complement(1591..>1892)
                     /locus_tag="ACHKIK_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9086307.1"
                     /translation="FQEARSAWGNCDWIGSGRMAIDGLKEVQEAVMLIEAGLSTYEKE
                     CAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQSTEEEKSDSRAA"
BASE COUNT          346 a          605 c          538 g          403 t
ORIGIN      
        1 tcgctggcag cctccggcca gagcacatcc tcataacgga acgtgccgga cttgtagaac
       61 gtcagcgtgg tgctggtctg gtcagcagca accgcaagaa tgccaacggc agcaccgtcg
      121 gtggtgccat cccacgcaac cagcttacgg gtggcggtgt ccagcatcag cggggtcatt
      181 gcagacgctt tcgcactcaa tccgccgggc gcggttgcgg tatgagccgg gtcactgttg
      241 cccagcggct ggtaatgggt aaaggtttct ttgctcgtca taaacatccc ttacactggt
      301 gtgttcagca aatcgttaac ggcatcagat gccgggttac ctgcagccag cggtgccggt
      361 gccccctgca tcagacgatc cagcgcagta tcactgcgcg cctgtgcact ctgtggtgct
      421 gcggccagaa tgcggcgggc cgtttccacg gtcataccgg gggtttctgc cagcacgcgg
      481 gcctgttctt cgcgtccgtg agcctcctca cagttgagga tccccataat gcggctgttt
      541 tctgccgcaa ccgcagcggt gatctgcgcg ttcacgtccg gctgcgccgc gctggcgttt
      601 tcgccctcca tcgctggcac cacgccagta acgtcagcct gcgaagcagt ggctgaaaca
      661 gttgttgatt gagtctcttt ggtcattcgc cctcctgaga gacgggattt acgtgcatcc
      721 agtgcatcac gcataacggt gatcgcatcg gtgctgttga caagttcatc agccagtccg
      781 gcatcaatgg cctcctgacc gctgtacact gcagcctcgg tatccagcac agcctgcacg
      841 gacaggccgg tatatgccga caccttctgc gcaaacatcc ggcgggttgc atccatccgg
      901 gactgcagtg tttcccggac atcacccggt agatggctgt aggggttgcc atccacctta
      961 tggctgccgc tgtaaatcag cgtgatttcc acgccctgtt tctccagggc agcaccgtaa
     1021 ttactgtgag ccatcatgac gccgatggag cctgtccggg cggtctgcgt gaccagacgc
     1081 cgggaggcgg cgctggcaag cagctgacct gcactgcagt tcatgtcgtt ggccagcgcc
     1141 cataccggtt ttatgtcacg cacacgggcg atgatgtcag cacagtcaaa tgctcccgcc
     1201 accattccgc ccggtgtgtc catatcgagc agaatgccgt ccaccatcgg gtcgctggca
     1261 gcctgttgca gacgggcgat aatgccgttg taaccggtca tccccgaata cggctgcagc
     1321 gcccgcgtcc ggctgaccag cgtaccggac accggcagca cggcgatgcc gttcatgacc
     1381 tgataactcc gggcctgtcg tggtccgtca tcatcaccgg ataacgccag cgccgcgggt
     1441 gcctctccgg cagtcaggct gtcgccggat actgcatccg tcaggcggct gatcccaagc
     1501 tggcctgcaa gcgcacaaaa gaaaacccgc gcataggcgg gttcaagcat cagcggctca
     1561 ttaaaagcca tgctggcaat atgcgggaga ttacgcagct ctgctgtcac tcttctcctc
     1621 ctctgttgat tgtcgcagcc cggattcaaa tgccgcagcc gcccaggcgg gcggtttaag
     1681 accggctgcg cggcgctcca tcgtttcacg gacctgctgg gcaaaaattt cctgatagtc
     1741 gtcaccgcgt ttcgcgcact ctttctcgta ggtgctcagt ccggcttcta tcagcatcac
     1801 cgcttcctga acttctttca gaccatcgat ggccatacga ccggagccta tccagtcgca
     1861 gttcccccag gcactgcggg cttcctgaaa gc
//
LOCUS       DAZGJW010000373         1883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZGJW010000373 DAZGJW010000000
VERSION     DAZGJW010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.376"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..741)
                     /gene="nleH1-2"
                     /locus_tag="ACHKIK_005381"
     CDS             complement(<1..741)
                     /gene="nleH1-2"
                     /locus_tag="ACHKIK_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HFO9086308.1"
                     /translation="MLSPSSINLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDSGTQV
                     TVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEAMLNTSPVSTTI
                     ERGSVHSNRPDLPPADYTQSELPRVSNNKSPVPGNVIGKGGNAVVYEDMEDTTKVLKM
                     FTISQSHEEVTSEVRCFNQYYGSGSAEKIYNDNGNVIGIRMNKINGESLLDIPSLPAQ
                     AEQAIYDMFDRLEKKGILFVDTTETNVLY"
     gene            complement(848..919)
                     /locus_tag="ACHKIK_005382"
                     /pseudo
     CDS             complement(848..919)
                     /locus_tag="ACHKIK_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            913..1883
                     /locus_tag="ACHKIK_005383"
                     /pseudo
     CDS             913..1883
                     /locus_tag="ACHKIK_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          565 a          450 c          385 g          483 t
ORIGIN      
        1 atataaaaca tttgtttctg ttgtatcaac aaaaagaatt ccttttttct ccagtctgtc
       61 aaacatatcg taaatagcct gttcagcttg tgctggtaat gatggaatat ccaaaagaga
      121 ttccccattt attttattca ttctaatacc aataacattt ccattatcat tatatatttt
      181 ctctgcactc ccggaaccat aatactgatt gaaacaacga acttcgcttg tcacctcttc
      241 atggctttga gatatagtaa acatcttcaa cacttttgtt gtatcttcca tatcttcata
      301 cacgacagca ttaccacctt taccaataac attacctggc acgggtgatt tattgttgct
      361 aaccctcggc aactctgatt gagtataatc cgctggaggt aaatcaggtc tgttcgaatg
      421 aacagagcct ctttctatag tcgtgctcac tggcgatgta ttcagcatag cctcaatttt
      481 tctgctaatc tctcccttag gccatcccaa cctgtgcaac agattagtaa aacaaccact
      541 atgactttct cttgtaacgc aaaacttatt atcagtgacc acaacacgat atgttctgtt
      601 gccaaccgtt acttgcgtcc cgctatcaga gtgaacagca gcatccctta cagaggataa
      661 aacacgatta tcaggcgaag tcaggtttct ggttaaagaa ttccatgaac atcccaaatt
      721 tatagaagag ggcgataaca tacatttcaa ccttcaaaat aaacctatct aattattcct
      781 aacagacatc ccccatacat gacaacaaaa accggagccg gactccggtt tttgtgaagc
      841 tatcgggtta cttcatttcg ccaatatttt cccacttccc gtcagcacgc aggatttgca
      901 gcggtcttac tacacactga cccgaggacg aaaaaagagt tcgggctggg aagagacaga
      961 agagtagcaa tttcagaagc attcacacca acattgagct tttcagcgaa agtgggcacg
     1021 aatcattgct ggacagggtt aaaggcactg actcaatcac tcttcatacg tggcttgatc
     1081 gatatgaaac aatcctcagc tagaggggga gcaagccgaa aacattactc gactacgcca
     1141 gcaaaatcag ggcaattcga agaaaattgc cggacaaacc gctcactgac atatcaacga
     1201 aagaggtggc agcaatgcta aacacctacg tcgcagaagg taaagcggtt tccgcaagag
     1261 taatcaggtc aacccttgtt gacgtttttc gaggggcaat agccgagggg catgtggcaa
     1321 cgaatccagt aacaacaacc cgtgcagcaa agtcagaagt aaggcgctca aggctgacag
     1381 ctaatgagta tgtcgctatt taccatgctg ctgagcacct ccccatctgg ctgaggctgt
     1441 caatggattt agctgtcgtt acagggcaga gagtgggcga tttgtgcaga atgaagtggt
     1501 cagacataaa cgatggtcat cttcacattg gacagagtaa aacaggagcc aaaattgcca
     1561 ttccgctggc tctaaccatt gacgcactcg acatctcact ggttgataca ctacagaaat
     1621 gcagggaggc cagcagcagt gaaacaataa tcgcatcaac ctatcacgaa ccactttctc
     1681 cagccacagt atcacggtat ttaacaaagg cgcgaaatgc atccgggatc tcgtttgatg
     1741 gagacccacc gacatttcat gaactacgta gtctgtccgc gaggctatat cggaaccaga
     1801 ttggctacaa gtttgcacaa cgtcttcttg gacataaatc tgattcaatg gcggcgcatt
     1861 atagggacag tcgcgggcgg gag
//
LOCUS       DAZGJW010000374         1873 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZGJW010000374 DAZGJW010000000
VERSION     DAZGJW010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.377"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(181..1020)
                     /locus_tag="ACHKIK_005384"
     CDS             complement(181..1020)
                     /locus_tag="ACHKIK_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO9086309.1"
                     /translation="MNDIENYGNRYHVLQSFPMPQLASSREIRILLPASYYESDVSYP
                     VLYMHDGQNLFDKSVSFGPHVWDIPEAVDAFFPNSQYDGVIIVGIDNASSHGKFSRMD
                     EYSPWPLNTSFPLPGWDPDVDYSGGKGALYVDFIVNTLKNYIDSNFRTLPDRNNTAIA
                     GSSMGAYISLFAAILRLDVFSKVGVFSPALWFNDSAMLNFIQENNIFADLTVYLDVGT
                     LETSGMREDFPEVYISGAEKLCVSLRKQRNVTIDYHLWGGDTHSESAWAKRFPEMLKL
                     FYC"
     gene            complement(1113..>1873)
                     /locus_tag="ACHKIK_005385"
     CDS             complement(1113..>1873)
                     /locus_tag="ACHKIK_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO9086310.1"
                     /translation="KIRMPEDVFLASLDKYNIDFALCSCGSAVEVDHDQNPIPDEDQV
                     TQHDNNERMLRLVRQHSKRIGAFMWIKPRLESCDQDFEDMIASNRDIIYGIKVHPYHS
                     KMAFNSDKVQEYIRLAQKYDLPVVSHTSNDYESSPQLVYEMALKYPNVNFVMCHMGLA
                     TDNQEAIELIAKLPNLYGDTAWVRADMVYQSIKICGSDKILFGTDNPINGLDTYNDDD
                     FYNKYFTELKSKLTQSEFEQLMFTNAKKLFKIKI"
BASE COUNT          615 a          393 c          310 g          555 t
ORIGIN      
        1 tttcttaaaa catcaattga ggcaatccag caggtcccaa ttttattaat aagtgtcgcc
       61 tcagcaccaa aaataatttt atcaacagtt ggttgcgaaa cggcaaccat tgaatttaaa
      121 aaacaattaa gatctgcggc tgttttaata tgatgcattc aacatccttc accggtttca
      181 tcagcaataa aataatttca acatttctgg aaaacgtttt gcccatgcag actcagagtg
      241 ggtatcacct ccccatagat gataatctat agttacattg cgttgtttac ggagactcac
      301 gcataatttt tccgcgcctg aaatatagac ttcaggaaag tcttcgcgca taccactggt
      361 ttccagcgta ccgacatcga gatatacagt aagatctgca aagatattgt tttcctgaat
      421 aaaattcagc attgcgctgt cattgaacca tagtgccggc gaaaaaacgc caacttttga
      481 aaaaacgtct agacgaagaa ttgcggcaaa caaactgatg tacgcaccca tagaactacc
      541 tgctatcgct gtattattac gatctgggag agttcgaaaa ttgctgtcaa tatagttctt
      601 gagcgtattc acaataaaat caacatacaa cgcacctttt cctccactat aatccacatc
      661 agggtcccag ccaggtaagg gaaaagatgt gtttagcggc catggagaat actcatccat
      721 acggctgaat ttaccatgag acgatgcatt atcaatcccc acaatgataa caccgtcgta
      781 ttgggaattt gggaaaaaag catctaccgc ctcaggtata tcccatacat gtggaccaaa
      841 agatacagat ttatcaaata aattttgccc atcgtgcata tacaatacag ggtaactgac
      901 atcagattca taataggacg caggtagtaa tattcttatt tcgcgactac ttgccaattg
      961 tggcatcgga aaggattgca acacatgata tcgattccca tagttctcaa tatcgttcat
     1021 aatttaaatt aacctcaata aacaaaagaa atattacatc taagcactcc cctaccacat
     1081 tatatatggg agcacttaga aatgactaat atctatattt ttatcttaaa aagttttttc
     1141 gcattggtaa acataagttg ttcaaactca ctttgcgtta atttactttt taattcagta
     1201 aaatacttat tataaaaatc atcatcatta taagtatcta gtccattaat aggattatcg
     1261 gtaccaaata aaatcttatc actaccacag atttttatcg actggtaaac catatccgcc
     1321 ctaacccacg cagtatcgcc atagagatta ggaagttttg caatcaattc gatcgcttcc
     1381 tgattatctg tcgcgagccc catatgacac ataacaaaat tcacatttgg atatttaagt
     1441 gccatctcat aaaccaactg tgggctggat tcataatcat tagaagtatg gctaactacc
     1501 gggagatcat atttttgagc aaggcgtata tattcctgaa ctttatctga attgaaagcc
     1561 atttttgaat gatatgggtg aactttgatg ccataaatga tatctcgatt gctggcaatc
     1621 atatcttcaa aatcttgatc acaactttcc agacgaggct taatccacat aaacgcgcca
     1681 attcgctttg aatgttgacg aacaagtctg agcatacgtt cattattatc atgctgtgta
     1741 acttgatctt catccggaat cgggttctgg tcatggtcaa cttctactgc agatccacaa
     1801 gaacataatg caaaatcaat attatacttg tccagagatg ccagaaaaac atcttctggc
     1861 atacggattt tca
//
LOCUS       DAZGJW010000375         1872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZGJW010000375 DAZGJW010000000
VERSION     DAZGJW010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.378"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..176)
                     /locus_tag="ACHKIK_005386"
     CDS             complement(<1..176)
                     /locus_tag="ACHKIK_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO9086311.1"
                     /translation="MRKLYAAILSAAICLTVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYE"
     gene            complement(244..>1872)
                     /locus_tag="ACHKIK_005387"
     CDS             complement(244..>1872)
                     /locus_tag="ACHKIK_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9086312.1"
                     /translation="IPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGS
                     GEDSRLVTTAITADTEHRSSGLPLGEYTLTVRAINSYGQQGEPATTTFRINAPAKPAT
                     IELTPGYFQITAVPRLAVYDPTVQFEFWFSETKIADTSQVETSARYLGTGSQWSVSGP
                     HIKPGKDFWFYVRSVNLVGKSAFVEASGQASNDAEGYLDFFRGEIGKTHLAQGLWELI
                     DNSQLADEMAEMKTSITETRNEITQTVNKTLEDQSATIQQIQRVQKDTNDDLAALYML
                     KVQKTKDGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPENGNSTPLFVAQGNQL
                     FMNDVFLKRLFAVSITSSGNPPTFSLTPEGRLTARNADISGNVNANSGTLNNVTINEN
                     CRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAV
                     AFSGAKHEREHTDIYSSCRLIVRKNGAEIYNCTALDNTLIYSGVIDMPAGHGHMTLEF
                     SVSAWLVNNWYPTASISDLLVVVMKKSTAGISIS"
BASE COUNT          394 a          547 c          462 g          469 t
ORIGIN      
        1 aattcataac ggtatttcac gttaatcccg ttaagattat cgctaccggg agcgttcgtc
       61 cgggcatgaa gataccccgc gctcagcgtg gcctgctgct cagacgccca tgcaggcgca
      121 ccggatacgg tcagacaaat ggctgcggac aaaatggcgg cataaagttt acgcataatt
      181 acctctcgct tttctgcaat aaaaaaaggc gtcatttctg acgcccgttc tgggttataa
      241 aattcagctg atactgatac ctgctgtgga ttttttcatc accacaacca gcagatcgct
      301 gatacttgct gtgggatacc agttatttac cagccatgct gacaccgaaa actccagcgt
      361 catgtgaccg tgaccggcag gcatatcaat aacgccactg taaatcagcg tattatccag
      421 cgcggtacag ttataaattt cagcaccgtt tttccgcact atcagacggc atgaggagta
      481 aatatcagta tgctctctct catgtttagc gccgctgaat gccaccgccg gaataacaat
      541 ttgccggtca aacggctgat cgtcataaac cctgacggta atggtccctg atggccaccg
      601 ctccggtgca cgggaatccc gggggaaagc tttgcccact gttttaacga gatcgccttc
      661 aatctggttc gcggacagtt ttcccagaac ccgacagttc tcgttaatcg tgacgttgtt
      721 gagcgtcccg gaattcgcat tcacgttacc gctgatatca gcatttcttg cggtcagcct
      781 gccctccggc gtcagggaaa acgtcggggg attgccggac gaggtgatac tcaccgcaaa
      841 cagccgcttc aggaacacgt cgttcatgaa cagctgattc ccctgcgcca caaataacgg
      901 cgtgctgttg ccgttctccg gatttatcat cgcgatacgg tcagccagca gcagtatgtt
      961 gctcagtggc tggccatcag tatcctcaat ccctgcacca atcccggcca catagggaat
     1021 gccgtctttc gttttttgaa ccttcagcat gtacagcgca gccaggtcat catttgtgtc
     1081 cttctgcacg cgctgtatct gctgtatggt ggcgctctgg tcctccagcg ttttgttgac
     1141 cgtctgtgtg atttcattgc gggtttccgt gatgctggtc ttcatctccg ccatctcatc
     1201 tgcaagctgg ctgttatcta tcagttccca caacccctga gccagatgcg tttttcctat
     1261 ttctcctctg aaaaagtcca gatacccttc tgcatcattg ctggcctgcc cgctggcttc
     1321 cacaaacgca gacttgccca ccagattgac gctgcgcacg taaaaccaga aatccttccc
     1381 gggcttaatg tgcgggccgg atacactcca ctgactgccg gtccccagat aacgggcaga
     1441 ggtttccacc tgagatgtgt ctgcgatttt tgtctccgaa aaccagaact caaactgtac
     1501 cgtcgggtca tacaccgcaa gacgcgggac cgccgttatc tgaaaatacc ccggcgtcag
     1561 ttcaatggtg gcgggttttg ctggcgcgtt aatccggaag gtggtggtgg ccggttcgcc
     1621 ctgctggcca taactgttaa ttgccctgac tgtcagggtg tattccccga gcggcagacc
     1681 actggaacga tgctctgtat ccgcagtgat ggcggtggtc accagacggc tgtcttctcc
     1741 gcttccgctg gtcagtcgca gactgaagcg cacccccttc accacccgcg gcgtgtccca
     1801 tttcgcctgt gccagatact ggccgtcagc cgcgctcacc tccaccgtga ggtgctgcac
     1861 tgccggtgga at
//
LOCUS       DAZGJW010000376         1855 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZGJW010000376 DAZGJW010000000
VERSION     DAZGJW010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.379"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1855
                     /locus_tag="ACHKIK_005388"
     CDS             <1..>1855
                     /locus_tag="ACHKIK_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin B"
                     /protein_id="HFO9086313.1"
                     /translation="IKKDFIPKKHYSENSLFSNYRMDSIIPKKYSALFISGPDAIMMA
                     MKDYYNSLGELGECRIDREARNFKGLAEDSFVGNLKVIEGAKGVHYDWVNANSVCVND
                     VTPDDASTWVAKLEKIEDIFSNVDKLKKDSGWNITPTEINISKLTVGWPSEIKKTIKE
                     KWPEMEADYNRIISGSTIDLEDISQIDQKISKYLISQTNSLVKWVGISLAEQLAVKIK
                     HLTLPIENKIHYLMSDIHKQFKDNVQSINSLLASDSNTVVYIWNDDNINKSLMLRELW
                     IIESRRQSIYKLLNGQHDKTKRRLLLEYLRLKEKEQLGIIDKNRQERLLIISTNLSED
                     DFLKAKLIDIENKALSSSLKKISDKSGNPINIYDIKDVDTLTKNLNGLYKKKHINMLW
                     DKYISKNTEKIKELYSALKESEFSDRVKVKEINVEFNDDSLIKSIIHDGYDFEDIERI
                     VKYSIISKESGVVIQNAAVSAPSKELVDIVSKYALTFGVDTTTILDKLYNKLFGIEEI
                     VRDSSKQSKIHHEIIAGVLSDIDTKNINRYFVSIMNQNVSGLGVKFSNTGNALSDDVI
                     ISGIKEIQDSKKDIVFENIKSYFSALYLTGTIIKNKGNINANLIKYIFKQKS"
BASE COUNT          738 a          222 c          335 g          560 t
ORIGIN      
        1 aataaaaaaa gacttcattc ctaaaaaaca ttattctgaa aatagtctct tttccaatta
       61 tcgaatggac tcaattatcc ccaaaaaata cagtgctctt tttatttctg gtccggatgc
      121 cattatgatg gcgatgaaag attactataa ttcattaggt gagctgggcg agtgccgaat
      181 tgatagagag gccagaaact tcaaaggatt ggcggaagat tcatttgtag ggaatcttaa
      241 agtgattgaa ggtgcaaaag gtgttcatta tgactgggtt aatgctaact cggtgtgtgt
      301 taatgatgtt acgcctgacg atgcaagtac ctgggttgct aaactagaga agatagaaga
      361 tatattttct aatgttgata aattaaagaa agattctgga tggaatataa caccaacaga
      421 aataaatata tcaaagttaa ctgttggttg gccatcagaa ataaaaaaaa caataaaaga
      481 aaagtggcct gaaatggagg ctgactataa taggataatt agtggtagta ctatagatct
      541 ggaagatata tctcaaatag atcaaaagat aagtaagtat ttgatatctc aaactaatag
      601 tcttgttaaa tgggtaggca tatcattagc agaacaactg gctgtgaaaa taaagcatct
      661 tacactccct attgaaaata agatacatta tcttatgtca gatattcata aacaatttaa
      721 agataacgtt cagtctataa actctctttt ggcaagtgat agtaataccg ttgtatatat
      781 ttggaacgat gataatataa ataaatctct catgttgagg gagttatgga taattgaatc
      841 cagaaggcaa tcaatttata aactgctaaa tggacaacat gataaaacga agagaaggtt
      901 attattggaa tatctaaggc tgaaagaaaa agaacagctt ggtattattg ataaaaatag
      961 acaagaaaga ttgttgatta tatcaacaaa tctttctgaa gatgatttct taaaggctaa
     1021 attaatcgac atagaaaaca aagcattgtc ttcatcattg aaaaaaatat cagataagag
     1081 cggaaaccct attaatatat atgatataaa agatgtcgat acactaacaa aaaatcttaa
     1141 tggattatat aagaaaaaac atattaatat gctatgggat aaatatatat caaagaatac
     1201 agagaaaatt aaagaactat atagtgcatt aaaagaaagc gaattttctg atagagttaa
     1261 agtaaaagaa ataaatgttg agtttaatga tgatagttta ataaaaagca taattcatga
     1321 cggttatgat tttgaggata tcgaaagaat tgttaagtat tcgatcatta gcaaagagtc
     1381 tggagttgtt atacaaaatg ctgccgtttc agcaccatca aaagagctag tcgatattgt
     1441 atccaaatat gcattaacgt tcggtgtgga taccactact attttagata aattatataa
     1501 taaattattc ggtatcgaag agattgtcag agattccagc aagcaaagca aaattcatca
     1561 tgagatcatt gctggagtat taagtgatat cgatacaaaa aatattaata gatattttgt
     1621 atcgatcatg aatcagaatg tgtctgggct gggagtaaag ttttctaata cgggtaatgc
     1681 tttgtctgat gatgtaatta tatctggcat taaagaaata caagatagta aaaaagatat
     1741 agtttttgaa aatattaaaa gttatttttc cgcattatat ttgactggaa ctataataaa
     1801 aaacaaaggt aatattaatg ccaatcttat aaaatatata ttcaagcaaa aatcc
//
LOCUS       DAZGJW010000377         1853 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZGJW010000377 DAZGJW010000000
VERSION     DAZGJW010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.380"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>1853)
                     /locus_tag="ACHKIK_005389"
     CDS             complement(<1..>1853)
                     /locus_tag="ACHKIK_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HFO9086314.1"
                     /translation="KDLFLTDNVGTGLLSKLQVQEELVLGILSAIHQRVSEKLRNINI
                     HKYRINSVNPNSEGDGVKLSLYDLDKNKNINVNIDTSDLNITIRQGLDSLTEAIDEMN
                     IDGIMAIVGIIQYMKITGYGGYVSAIDHANFVSDIKTVVEKVVGTSLMFMGVKKFGTN
                     VSQIRLETMASITLSQVATRLGGVQGRILSKIANVIKFPVIDTALNLWSLGESIHRYT
                     SEKSGSLDKMLAEIDVAFASTYTALTLSSFAFPPIALATLPLIFLQQDIKTFKVHLHM
                     ENERRDAWKKVEHYLDTAARRNVVKVDTFRGVIDLSAIDIIGGLKLDLSTENVKLSGN
                     LSYNRGKNIGNDPNLSDIDVRKISKYAVACVNDDDLHVPSFGGENRRERCRELSLSES
                     GLVRGFANRIWPSHVPMIKKGNYNTVILGYSSQIVANTEVIRMAWKDFQEVARENYPL
                     VEVINKNAEVVTGNKNVKVILPVLEHDIFSPKNSEDLYNLSTHWFTVRGGEKGVTVFP
                     NGVGNFNIVGALGAKNILSFSQLPEGFNVFVNLNQTDKQTVAVYQSKAELNMNLIQKN
                     INTIIGSDYGVNVFIGNKLDNHFVTGRSGATIYPGQGSNIITIPKNINNFF"
BASE COUNT          580 a          369 c          260 g          644 t
ORIGIN      
        1 gaaaaaatta ttaatattct taggaatggt tattatattt gagccttgtc ctgggtatat
       61 agttgcgcct gagcgccctg tgacaaagtg attatctaat ttattaccaa taaaaacatt
      121 aaccccataa tcagaaccta taatggtatt tatatttttt tgaattaggt tcatgtttaa
      181 ttcagcttta gattgataaa cagccacggt ttgtttatct gtttggttta aattgacaaa
      241 aacattaaaa ccttctggta attgactaaa tgatagtatg ttttttgctc ccaacgcacc
      301 tactatatta aaattaccaa ccccattggg gaaaacagta acaccttttt caccacctct
      361 gacagtgaac caatgcgtac ttaaattata taagtcttca ctatttttag gagagaaaat
      421 atcatgttcc aatacaggaa gaattacctt aacattttta ttgcctgtaa caacctcagc
      481 atttttattt attacttcaa ccaaaggata attttctctt gcaacctctt gaaaatcttt
      541 ccaagccatt ctaattactt ctgtattagc cacaatttga gaggaatacc ccaatatcac
      601 ggtattataa ttcccttttt ttatcatcgg tacatgtgaa ggccatatcc tgtttgcaaa
      661 accgcgaact aatcctgatt ctgataaaga tagttctcgg catctttccc ttctattctc
      721 cccgccaaaa ctaggaacat gcaggtcatc atcattaaca caagcaacag catatttact
      781 tatttttcta acatctatat cactcaggtt tggatcattc ccaatatttt ttcctctgtt
      841 atatgagaga ttaccggata atttcacatt ttctgttgaa agatctagtt ttaaaccacc
      901 aattatatca attgccgaaa ggtctattac accccggaaa gtatcaactt taacaacatt
      961 acgtcttgca gcagtatcta gatagtgctc aacttttttc caagcgtctc ttctctcatt
     1021 ttccatgtga agatgtactt taaacgtctt tatgtcttgt tgtaaaaaaa taagcggcag
     1081 agtggctaat gcgatgggtg ggaatgcaaa actagatagt gtaagcgcgg tataagttga
     1141 tgcaaaagca acatctattt ctgctaacat tttatctaaa cttccacttt tctccgatgt
     1201 gtatctgtga atagattcac caagagacca taaatttaat gctgtatcga tgacaggaaa
     1261 cttaattaca ttagctatct tagataaaat tcttccttga actccaccaa gtcttgttgc
     1321 aacttgagat aacgtaatag aagccattgt ttctaaacga atctgagaga cgttggtgcc
     1381 gaattttttt actcccataa acatcaggct cgtgcccaca actttctcta ctactgtctt
     1441 tatatctgac acgaaattcg catggtctat cgctgataca taaccaccat atcccgtaat
     1501 tttcatatat tgaataatac cgacaatagc cataatccca tcaatattca tttcatcaat
     1561 ggcttctgtt aatgaatcaa ggccttgtct gatcgttata tttaaatctg aagtatcaat
     1621 attaacattt atatttttat tcttatctaa atcatacagc gatagtttaa caccatcacc
     1681 ttcactatta ggattaaccg aattaattct atatttatgg atatttatat tgcgtaattt
     1741 ttctgatact ctctggtgaa tagcacttaa gattcccaaa accaattctt cttgaacttg
     1801 aagcttactt agtaatccag taccaacgtt atctgttagg aatagatcct taa
//
LOCUS       DAZGJW010000378         1845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZGJW010000378 DAZGJW010000000
VERSION     DAZGJW010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.381"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1273)
                     /locus_tag="ACHKIK_005390"
     CDS             complement(<1..1273)
                     /locus_tag="ACHKIK_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFO9086315.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFY"
     gene            complement(1362..>1845)
                     /locus_tag="ACHKIK_005391"
     CDS             complement(1362..>1845)
                     /locus_tag="ACHKIK_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrmB family transcriptional regulator
                     sugar-binding domain-containing protein"
                     /protein_id="HFO9086316.1"
                     /translation="NVSGFDNLHQKAKEMLSSAECEIYLNTDFDLHFLKNEICEALNR
                     HVRVIVFSFNKLITPDPRVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGL
                     YSNNRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR
                     "
BASE COUNT          581 a          360 c          328 g          576 t
ORIGIN      
        1 cataaaataa tgcagtaaaa ccattgttat tttcttgaaa catattttcg acaaaagccg
       61 gtatggttct tgctgtattc ggtacgcagc agacagcagc ctcctcatga gtaggggagt
      121 atttatatct ggtattaaac tgattactgt ccggatgttt cctttcattt gcttcataac
      181 aattatcagt cttacagtac attatcgcgt gaagtatggg atgctgcatg ccaaaactag
      241 cattatagaa tagccactca gcatcatcgg ccagaccaat attagtggtc agttttatta
      301 acataacata agtgctaaat agttcatgaa cagaacaata ttcatagcct gtataagtgg
      361 cattagctgt gcgtcctgta atccactcat caccaatggg agcgccagat ggtgtaagat
      421 aatatttcaa ctgggataaa aaaatatcta atgcagtttg atactgcggc cgttggctcg
      481 ctgcgatggc taatgcccta atatgctcat acgtatgtac gccatgacct ttaagaaggt
      541 aaccagggtc tatgacattt ttatattgca gatcttgctc cgacacgcat cctgcggaat
      601 aatcttcata cagccacaag caatatgaga gatattcaat gttattggtt atttcataaa
      661 ggcggtagaa aacatctgtt attgttaatc cgtggctatg tccagcaaaa ccctgttcca
      721 caacgaaagg atgtgacgtt tctttagggt agccagccat aatgcattgt gcggcttggg
      781 taagagaagt taataaactc tcattacggg tggactcata acatccaagc acaaaacgca
      841 aggcagtagc ttgtgcccag aactctccat tttctgcttt acagttaaat tttaatgatg
      901 aatcataaat tccaatatag ccatcatcat cttgcgtatc gataagttcc aaacataaat
      961 cgctcgcttt ttcctgccat tctttattat ctaacaagta ggcactgcgg caatatccat
     1021 ccttccaatt cgattgagtt tcactatccc accaataata ttgcatcatg ttatcatcgg
     1081 gattattctg ttttttaata acgccatctt cagcagatac agtgcgtgga gttatacgat
     1141 ctcttccata tattttatgc tccgtaataa gatggggaac taatttatcc agttctccta
     1201 tatgaccgtt catataccat tccatcttat tctttaacca accagtgggc tttatatcgt
     1261 taaaaaaaat cataaatata cctttgcata aaaaagttta gacgttataa aagacaaata
     1321 gattgtgatc gcctacagac gatcacaatg aaaggaaaat attaacggtt tttgctatca
     1381 ctcaaatgtt tttcatcctg caccatatca ttgccagatt catgcaaagt atttattaat
     1441 gcattggcat ctggatattg ttgctgatac ttggttagat atatatcact atggatatgt
     1501 tcggcaatta tattgataag caagcgatta ttcgagtata accccagtga ttcagctcgt
     1561 tgtgaaaaaa ccattactgt cttcatatca acaacaagca taaaacggtg agaagggtaa
     1621 tattcttctt catggttaaa gcggtaatac agttctacac gtgggtcggg agtaataagt
     1681 ttattaaatg aaaaaacaat aactcgaaca tgtcgattca gagcctcaca aatttcattt
     1741 ttcaaaaaat gtaagtcaaa atccgtgttg aggtaaattt cacactctgc agaagaaagc
     1801 atctctttag ctttctgatg taagttatcg aaaccagaga catta
//
LOCUS       DAZGJW010000379         1835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZGJW010000379 DAZGJW010000000
VERSION     DAZGJW010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.382"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1285
                     /locus_tag="ACHKIK_005392"
     CDS             <1..1285
                     /locus_tag="ACHKIK_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO9086317.1"
                     /translation="WNTPLGAISFYAARSSSKLNNDTRHEGTSYQVAYNKYLVQTATR
                     FSVAAWRYASQDYRTFSDHLYENDKHYHQSDYDDFYDIGRKNSLSANIMQPLSNNLGN
                     VSLSALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYDENNKEEERFNLFISIP
                     FYWGDDIAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNND
                     TSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAILD
                     APGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQGNRQIIAP
                     YRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVD
                     EIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            1298..>1835
                     /locus_tag="ACHKIK_005393"
     CDS             1298..>1835
                     /locus_tag="ACHKIK_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO9086318.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECTDLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLM"
BASE COUNT          586 a          354 c          350 g          545 t
ORIGIN      
        1 ttggaataca ccgctgggtg ccatctcttt ttacgctgcc cgatcgagta gtaagttaaa
       61 taatgatacc agacacgaag gtacgagcta tcaggtcgcc tataataaat atctagtaca
      121 aacagccact cgatttagcg tggccgcctg gcgttatgct tcgcaggatt acaggacatt
      181 cagcgaccat ctttacgaaa atgataaaca ctatcatcag agtgattatg atgattttta
      241 tgatattggc agaaaaaata gcctttctgc taatattatg caacctttat ccaataatct
      301 gggaaatgta tcattaagtg cgctttggcg gaattactgg gggcgaagtg gaaatgctaa
      361 agattaccaa ttcagttact ccaataactg gcaacacatt agttatactt tctctgccag
      421 ccaatcttat gatgaaaata ataaagaaga ggagcgtttt aatctgttta tctccattcc
      481 tttctactgg ggggatgata ttgccaaaac acgtcaccaa attaacttat cgaattcgac
      541 ctcattttcc aaagatggct attcctccaa caatactgga attactggca tagccggtga
      601 acatgatcag ttaaattatg gtatatatgt taatcagcaa caacaaaata atgatacctc
      661 gcttggtacg aatttaagct ggagaactcc catcgccata atagatggca gctatagtca
      721 ttctaaaaac gcctggcaaa gtggtggaag tattagtagt ggattagttg tctggcccgg
      781 tggtattaat atcactaacc agttatccga tacatttgca attctggatg cgcctgggtt
      841 agaaggtgca catattaatg gacaaaaata caatcgaaca aacagcaaag gccaggttgt
      901 ttacgacctg ataatacctc atcgtgaaaa ccatctggta cttgatatag caaacagtga
      961 aagtgaaaca gaattgcagg gcaatcgtca aattattgca ccttaccgtg gagcagtttc
     1021 ttatgtgcag tttacaactg accaacgtaa gccctggtat atacaggcac tgcgtcccga
     1081 tggttcgcca ttaacctttg gctatgacgt actggatctc caggaaaata atattggagt
     1141 cgttggccag ggtagtcgcc tttttattcg cgtagatgaa attcccactg gcataaaagt
     1201 tgctctcaat gatgaacaga acttattctg tactattact tttcaacacg ttatcgatga
     1261 aaacaaaaca tatatatgcc agtaaaagga gtttactatg aagtggcttt tattaatcac
     1321 cttgagtcta tattctttta ttgtgcaaag tgctccctgt gcattgacca atgtcggtga
     1381 acaacgtgga acatatatac tcaagccact gagtatgaaa ggtaatctaa ccgccgaaaa
     1441 aattgaaaac accggaaata tcattgagtg tacagacttg caagagacaa ccaacgccat
     1501 taaagttcat tttttaacac agaacgcttt aaggtttaaa gtcggaagtc gtcattatat
     1561 tattaatttc ccctttgaat ctgacggtaa aaaaatagag cttccaggga agtcatctta
     1621 taatttagaa gaaatgctat ctgcgataaa tttcaccatt ccatattcta tcgcatctgt
     1681 atcagatagt tctaacaccg cagacatttt tcctggaact ccttttcctt tgttgttcga
     1741 aattgaagtg agttcatgta cagggaataa tactaattgt aatagcgtcc gctttaatta
     1801 taatttaaat actattttac aggtaaattt aatga
//
LOCUS       DAZGJW010000380         1815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZGJW010000380 DAZGJW010000000
VERSION     DAZGJW010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.384"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..103
                     /locus_tag="ACHKIK_005394"
     CDS             <1..103
                     /locus_tag="ACHKIK_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFO9086319.1"
                     /translation="KPMPSREELLKRKSFGSVNDNKYLNAMWRSGKK"
     gene            100..657
                     /locus_tag="ACHKIK_005395"
     CDS             100..657
                     /locus_tag="ACHKIK_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086320.1"
                     /translation="MKQMTLIEMDGFLKGKCIPSDLKVDETNDEYLVRKFGELESKLE
                     TALRECRSAGITIDNLEAKCAKMAAENSSLKQSEKEFNDFCREEFSEWEDDITETPAT
                     DAFLAEVKTEARKEGAYFVANRMLAAWEAGFIDDTAKNAADIARMILTSTEFMANAPE
                     GDFDRSFSDGVLEDIAEQLRKGVIQ"
     gene            654..1211
                     /locus_tag="ACHKIK_005396"
     CDS             654..1211
                     /locus_tag="ACHKIK_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO9086321.1"
                     /translation="MSKIDYQALREAAERAIPAMERLLMLPVDDDLLTEQELKDYGVD
                     IDALNAFKFLTGPETVLALLDERERNQQYIKRRDQKNEDIALTVGKLRVELEAVQKTS
                     AARIEAIDRTHKMFQREKDRADAAEKCIAELSASHSKLRDTMAGIHNTIRMDGGYTPL
                     AAILNAAKRAYEESASAAGIRIKGE"
     gene            1213..1350
                     /locus_tag="ACHKIK_005397"
                     /pseudo
     CDS             1213..1350
                     /locus_tag="ACHKIK_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          528 a          387 c          479 g          421 t
ORIGIN      
        1 aaagccaatg ccatcgagag aggaattgct taagagaaag agtttcggtt ctgttaatga
       61 caacaaatat ctgaatgcga tgtggcggag tgggaagaaa tgaaacaaat gacactaatt
      121 gagatggatg gttttctgaa aggtaaatgc atcccaagtg atttaaaggt tgacgaaaca
      181 aacgatgaat atcttgtccg taagttcggt gaacttgaat caaaactaga aacggcgttg
      241 cgggagtgtc gttctgctgg aatcacgatt gataaccttg aggctaaatg cgcgaagatg
      301 gctgctgaaa attcctcgct taagcaatct gagaaggaat ttaatgactt ttgtcgtgag
      361 gagtttagcg aatgggaaga tgatattact gaaaccccag ccaccgatgc ttttctggct
      421 gaagtgaaga ctgaagcacg caaggagggc gcttactttg tggcgaacag aatgctggct
      481 gcctgggaag ctggttttat tgatgatact gcgaagaacg ccgctgatat tgcccggatg
      541 attcttacct ctactgagtt tatggccaat gcgccggaag gcgattttga ccgttcattc
      601 tctgatggcg ttctcgaaga tatcgccgaa cagcttcgta aaggagtcat ccaatgagca
      661 agattgacta tcaggcactg cgtgaggcgg cagagcgtgc aattccggca atggaacgcc
      721 tgttaatgtt gccagttgat gatgatttgt taactgaaca ggaacttaag gattacggtg
      781 tggatattga tgcgctcaac gccttcaaat ttctgaccgg gccagaaacc gtgctggcac
      841 tactggatga acgggaaaga aaccagcaat acatcaaacg ccgcgaccag aagaacgagg
      901 atattgctct tacggttggg aagctgcgtg ttgagcttga agccgtgcag aagacatcag
      961 cggcacgaat cgaagctata gaccgcactc acaaaatgtt ccagcgtgaa aaagatcgag
     1021 ccgatgccgc agagaagtgc atagcagaac tgtctgctag ccacagcaaa ttgcgcgaca
     1081 caatggctgg catccacaac acaatccgaa tggatggcgg ctatacgcca ctggcagcaa
     1141 tccttaacgc tgctaaacgc gcatatgaag aatcagcaag cgcagctggc attcgcatca
     1201 aaggagagtg atatgagcac tatcactaaa gaacgtatcg aattgttcat taaatccccg
     1261 cttgataacg gacttactag tggcgaacaa atggaactgg cacggattgc tctggcatcg
     1321 ctggaagcaa aaccaatagg tgcattccac attgcagaac agcaagttga cggcacaagt
     1381 gactacctca aggatggaga atggcctatt gataatggaa ttattgaggt ctacgccgct
     1441 ccgccagtac cggtagtacc tgctgcatta cctgagaacg acgatgagga cgggcatgac
     1501 attgattatc ttgatccatc tgaagtttac gcgcttgggc gaacagctgg ctggaacgcc
     1561 tgccgcgccg ccatgcttca gggtaaatca gaacaaccac aaaacgcaca acaaaatatt
     1621 ccggaaaata ttcccggtgg caactctccg gtaactccgg atggttgggt tatggtgccg
     1681 aaggagttaa ccccggaaat gatgagagcc gttcagatta gaagtgagct tggagggtat
     1741 gctacttcca atctatctgg tgcatacaat atgttttctg agttctggaa tgttgctgtc
     1801 tcagctgcgc ctaag
//
LOCUS       DAZGJW010000381         1813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZGJW010000381 DAZGJW010000000
VERSION     DAZGJW010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.385"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            18..65
                     /locus_tag="ACHKIK_005398"
     CDS             18..65
                     /locus_tag="ACHKIK_005398"
                     /inference="COORDINATES: protein motif:HMM:NF017611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO9086322.1"
                     /translation="MSDPNSSMYEEEYDG"
     gene            58..657
                     /locus_tag="ACHKIK_005399"
     CDS             58..657
                     /locus_tag="ACHKIK_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO9086323.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALERRSKERE
                     KAEKAAEKKRRREKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISCGT
                     LTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQEAV
                     DEIESNHSRHRWTIEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            654..860
                     /locus_tag="ACHKIK_005400"
     CDS             654..860
                     /locus_tag="ACHKIK_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO9086324.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            838..1503
                     /locus_tag="ACHKIK_005401"
     CDS             838..1503
                     /locus_tag="ACHKIK_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO9086325.1"
                     /translation="MRYYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKAGELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQLIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            1500..>1813
                     /locus_tag="ACHKIK_005402"
     CDS             1500..>1813
                     /locus_tag="ACHKIK_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO9086326.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYA"
BASE COUNT          585 a          404 c          455 g          369 t
ORIGIN      
        1 actgctgtgc agaactgatg agcgatccga atagctcaat gtacgaggaa gaatacgatg
       61 gctaaaccag cgagaaggaa atgcaaaata tgcaaggaat ggtttcaccc ggcattctca
      121 aatcagtggt ggtgctgccc ggaacacgga actcaattag cactcgaacg acgaagtaaa
      181 gaacgcgaaa aagcggaaaa agcagcagag aagaaacgac gacgagagaa acagaaagat
      241 aaacttaaga ttcgaaaact cgccttaaag ccccgcagtt actggattaa acaagcccaa
      301 caagctgtaa acgccttcat cagagaaaga gaccgcgact taccatgtat ctcgtgcgga
      361 acgctcacgt ctgctcagtg ggatgccgga cattaccgga caactgctgc ggcacctcaa
      421 ctccgatttg atgaacgcaa tattcacaag caatgcgtgg tgtgcaacca gcacaaaagc
      481 ggaaatctcg ttccgtatcg cgtcgaactg attaaccgca tagggcagga agcagtagac
      541 gaaatcgaat caaaccatag ccgccatcgc tggactatcg aagagtgcaa ggcgatcaag
      601 gcagagtacc aacagaaact caaagacctg cgaaatagca gaagtgaggc cgcatgacgt
      661 tctcagtaaa aaccattcca gacatgctcg ttgaagcata cggaaaccag acagaagtag
      721 cacgcagact gaaatgtagt cgcggcacgg taagaaaata cgttgatgat aaagacggga
      781 aaatgcacgc catcgtcaac gacgttctta tggttcatcg cggatggagt gaaagagatg
      841 cgctattacg aaagaattga tggcagcaaa taccgaaata tttgggtagt tggcgatctg
      901 cacggatgct acacgaacct gatgaaaaaa ctggagacga taggattcga caccaaaaaa
      961 gacctgctta tctcggttgg cgatttggtc gatcgcggta cagagaacgt cgaatgtctg
     1021 gaattaatca cattcccctg gttcagagct gtacgtggaa accatgagca aatgatgatt
     1081 gatggcttat cagagcgtgg aaacgtcaat cactggctgc ttaatggcgg tggctggttc
     1141 tttaatctcg attacgacaa agaaattctg gctaaagctc ttgcccataa agcaggtgaa
     1201 cttccgttaa tcatcgaact ggtgagcaaa gataaaaaat atgtcatctg ccacgccgat
     1261 tatccttgtg acgaatacga gtttggaaag ccagttgatc atcagcagtt aatctggaac
     1321 cgcgaacgaa tcagcaactc acaagacggg atcgtgaaag aaatcaaagg cgcggacacg
     1381 ttcatctttg gtcatacgcc agcagtgaaa ccactcaaat ttgccaacca gatgtatatc
     1441 gatactggcg cagtgttctg cggaaacctc acattgattc aggtacaggg agaaggcgca
     1501 tgagactcga aagcgtagct aaatttcatt cgccaaaaag cccgatgatg agcgattcac
     1561 cacgggctac ggcttctgac tctctttctg gtactgatgt gatggctgct atggggatgg
     1621 cgcaatcaca agccggattc ggaatggctg cattctgcgg taagcatgaa ctcagccaga
     1681 acgacaaaca aaaggctatc aactatctga tgcaatttgc acacaaggta tcggggaaat
     1741 accgtggtgt ggcaaagctc gaaggaaata ctaaggcaaa ggtactgcaa gtgctcgcaa
     1801 cattcgctta tgc
//
LOCUS       DAZGJW010000382         1807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZGJW010000382 DAZGJW010000000
VERSION     DAZGJW010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.386"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..123)
                     /locus_tag="ACHKIK_005403"
     CDS             complement(<1..123)
                     /locus_tag="ACHKIK_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO9086327.1"
                     /translation="MKTKKVMMIALVSSALALSGCGAMGTAIKKRNLEVKTQMSE"
     gene            546..809
                     /locus_tag="ACHKIK_005404"
     CDS             546..809
                     /locus_tag="ACHKIK_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO9086328.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            1111..1251
                     /locus_tag="ACHKIK_005405"
     CDS             1111..1251
                     /locus_tag="ACHKIK_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9086329.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
BASE COUNT          489 a          366 c          437 g          515 t
ORIGIN      
        1 ttcactcatc tgggttttta cttccagatt tcgttttttg atcgctgtgc ccatggctcc
       61 acatcctgaa agagccagcg cagagctgac cagtgcaatc atcataactt ttttggtttt
      121 catttaatgc ccttcttatc cgacaggttt atggaagaca gagaaccgcc tttacggtat
      181 atttttcagc gggcttctca agtatgttaa aggatacagg taaaaacagc gggatcagat
      241 acgtcgggta aacgtttatt tcataagaag tttttaggtc agaataaaat aatgggtgtt
      301 gttatattat cagatgatga tttatgtgag cgcgttacgg atattgcaca tgaggttaaa
      361 ggagagagca agcctccgca acggctcgct tcttatttaa tggatttttt cgggaagtcc
      421 agtttataat tatataacat attgtaatac tgaaagagag tgaggttata agaacagaca
      481 gttgaatctg accgtctgaa tatgtacctt tatgcggaat tttcacttaa agggggaaaa
      541 ctgttgtgaa accgaatatc catccggaat accgtacagt agtatttcac gataccagta
      601 ttgatgagta cttcaggatc ggttcgacga tcaaaactga ccgggtaatt gaactggatg
      661 gtgtaactta cccatatgtt acaattgacg tttcttctaa gtcgcatccg ttttatacag
      721 gtaaactgag aactgtcgct tcagaaggta atgttgcacg ctttacccag cgttttgggc
      781 gttttgttga tgcgaaaaaa ggtggctgat tttgccgcca gggtgcgttt gacaatttaa
      841 aaacgcacca gacagagaga tcctgcacct gaatataccg ttagcccgtc ccaccggggc
      901 gtgtgcagat gatctcgtca ccggaagatt gcggacctct ctgaaaacca gtacattacg
      961 cgcgggtgcc tttcggctga cggctggaag gattacctga aggccggatg tggaaaggcc
     1021 ccgagtcaac ttaaacgtta acccgaggcc ctgaccatct accttcagca agtggaaggt
     1081 tagcgcctct ctgtaaaagg agcaagtgct atgccgcaaa aaacgatcat tgtgggcatg
     1141 ttatgcctga ccatgttgct gaccgtctgg gtgttacacg catcaccgtg tgaattcagg
     1201 gtaagcttca tgtggtcgga aattgcggcg ttcttgcagt gtaaaccgta agaaaccgca
     1261 gcggggacaa cagtccccgc tatccggttg ccaaaggttc ggtcgaaagt cgcacccttg
     1321 cacacaaatc acaaagaaaa aacaaaagtc cgcagtgtaa aaactgtggg cttttttatt
     1381 cgcatcccga aagtgtggta acagcgtggg ggcaccaaaa aaagccccat aaaatggggc
     1441 tgaagattct gtaaggaatt tcacagcaat gagattcctg ctttctccaa ggatatacgt
     1501 gtcttcaggc tgaatcaatg ggggcgtggc tctgaatcca cctgcgatta aaacttgcac
     1561 aatgccggga gttgagttag aaaagtacag ggcgcgatat ccggcttatt ttgttgctgt
     1621 gttttgcctc gtgctggatg tcgcgtccac cgtttttctg atgattcata tgaaatggta
     1681 ctttattgat ttactgccat tctgatttgc ctttttatgt tcattattgc ggttgtcact
     1741 tccagtttct ggtgatgttt tgataaaagt gaaagaataa aacgataatt gcagaaggta
     1801 ttgctaa
//
LOCUS       DAZGJW010000383         1804 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZGJW010000383 DAZGJW010000000
VERSION     DAZGJW010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.387"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            84..386
                     /locus_tag="ACHKIK_005406"
     CDS             84..386
                     /locus_tag="ACHKIK_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepZ/EspZ family type III secretion system
                     protein"
                     /protein_id="HFO9086330.1"
                     /translation="MDAANLSPSGGVLPLAATINGNVSVDENTGVMIPENGVSRNIRI
                     AAGIALATTALAALGTGIAMACTTDASSQEFLGLGIATGVLGGVTALGGGLAMKYA"
     gene            470..847
                     /gene="sctI"
                     /locus_tag="ACHKIK_005407"
     CDS             470..847
                     /gene="sctI"
                     /locus_tag="ACHKIK_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO9086331.1"
                     /translation="MNNINQSENINIHFNKVSQTNFVDDHTPLAPTPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNTDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            849..1421
                     /gene="escJ"
                     /locus_tag="ACHKIK_005408"
     CDS             849..1421
                     /gene="escJ"
                     /locus_tag="ACHKIK_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFO9086332.1"
                     /translation="MRKYIKNTFLFVFICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNVTISVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            1427..>1804
                     /gene="sepD"
                     /locus_tag="ACHKIK_005409"
     CDS             1427..>1804
                     /gene="sepD"
                     /locus_tag="ACHKIK_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFO9086333.1"
                     /translation="MNNNNGIVKNDSDWLTALDFVKDVKGSPKHLTFHIYQKNAFLHD
                     FGSYWVLYIELNGDFRQVPTDTFIRLCNILAVSKEYKQMGIFQSNKKWYLCQLFNKDN
                     NHRANMSKAIMQHTLASLLDKQFD"
BASE COUNT          620 a          331 c          343 g          510 t
ORIGIN      
        1 taaataatat aatagttgcc catgggataa tttggttatt gataaccaga caaatgtcta
       61 aattagacaa tggagaaaag ttaatggatg cagcaaattt aagtccttct ggcggagtat
      121 taccactggc agccacaatc aatggaaatg tttcggtgga tgaaaataca ggagtaatga
      181 taccagaaaa tggggtaagt cgtaatatca gaattgcagc cggaattgca ctcgctacta
      241 cagctctggc tgctttaggt actggtatcg caatggcatg cacaacagat gcaagctccc
      301 aagaattctt aggtttaggt attgctaccg gtgtattagg tggtgttact gcgcttggcg
      361 gcggattagc gatgaaatat gcctaatcat cgcatcgcat aaataaaata gagaggtcat
      421 ggatgcatta tgccattacc tctctcatga aaagagacta accgtaaata tgaacaatat
      481 taatcaatca gaaaatatta atattcattt taataaagta tcccaaacaa atttcgttga
      541 tgaccatacc ccgttagcac caacgccctc tgcggctgga gcggctcagt ttcttgatca
      601 attacttcct aaaacagctg gtgtatcttc tccagagcaa gttttaattg aagaaattaa
      661 aaagagacat cttgcaacaa tgaacaccga tctcagtttc gatgccctat ctgcaggtgg
      721 actctcgcca gaagatgtgc tcacattaca aaagaatgtg ctcaacgcaa acgtcaatgt
      781 tgatgtcgta tccaagttag caagtcttct ttcaacatcg gttacaaaac tggtttcaat
      841 gcaataaaat gagaaaatac attaaaaaca cctttttatt tgtttttatt tgtttgacag
      901 ttgcatgtaa agagcaattg tatacaggat taacggaaaa agaagccaat cagatgcaag
      961 cattgttgtt atccaatgat gtcaatgtat ctaaagaaat ggacaaaagt ggaaatgtaa
     1021 caataagtgt ggaaaaagag gattttgtcc gtgcgataac cattttaaat aacaatggct
     1081 tcccgaaaaa gaaatttgca gatattgaag ttatttttcc gcccagtcag ctagtggcat
     1141 cgccatcaca agaaaatgca aaaatcaact atctcaaaga acaagatata gaaaggttac
     1201 taagtaaaat ccccggtgtt atcgattgca gcgtttcatt aaatgttaac aataacgaga
     1261 gccaaccatc ctcagcggct gttttagtca ttagctcgcc agaggttaac ttagcaccat
     1321 cagtcattca gataaaaaac ttagttaaga atagcgtaga cgatctcaaa ctagaaaata
     1381 ttagtgtcgt tatcaagtca tcctcaggac aggacgggta atatgtatga acaataataa
     1441 tggcattgtt aagaatgata gtgactggct aacggctctt gattttgtaa aagatgtcaa
     1501 aggttcacca aagcatctta cttttcatat ctatcaaaaa aatgccttct tacacgactt
     1561 tggaagttac tgggttctgt atatcgagct taatggcgat tttcgccagg ttccaacaga
     1621 taccttcatc agactctgta atattctggc cgttagtaaa gaatataaac aaatgggcat
     1681 ttttcaatct aataaaaaat ggtatctttg ccaactgttt aataaagata ataatcatcg
     1741 cgcaaacatg tcaaaagcaa taatgcaaca cacactcgcc tctttgcttg acaagcaatt
     1801 tgat
//
LOCUS       DAZGJW010000384         1789 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZGJW010000384 DAZGJW010000000
VERSION     DAZGJW010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.388"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(38..340)
                     /locus_tag="ACHKIK_005410"
     CDS             complement(38..340)
                     /locus_tag="ACHKIK_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9086334.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRTAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            438..809
                     /locus_tag="ACHKIK_005411"
     CDS             438..809
                     /locus_tag="ACHKIK_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086335.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGVSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            830..1021
                     /locus_tag="ACHKIK_005412"
     CDS             830..1021
                     /locus_tag="ACHKIK_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFO9086336.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            1023..1301
                     /locus_tag="ACHKIK_005413"
     CDS             1023..1301
                     /locus_tag="ACHKIK_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9086337.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
     gene            1588..1740
                     /locus_tag="ACHKIK_005414"
     CDS             1588..1740
                     /locus_tag="ACHKIK_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO9086338.1"
                     /translation="MDTIDLGNNASLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LAKNTS"
BASE COUNT          537 a          406 c          399 g          447 t
ORIGIN      
        1 acattcatcg ccatgcggat gtgttcatgc ttgattttca tgaatcaact ccatcagata
       61 agcatgcact acaatcacct tcagcatgaa ctacatgtgt ttgccccaaa cgaatgccgc
      121 tcgcatactc aggccaaata agctcccaat catgaggtcg tagctccgcc ctacttactt
      181 ggccttccgt cgcagattcg atcataaggg cgcgggttgg agatatagct gttcgtccag
      241 acgccatttg cgataagtaa gatggcgata caccaagtct ggccgcgaat ttcttagcat
      301 caccaaccct taatgattta ataaactctt ttaatgtcat accttcctcg gtttagtgtt
      361 tttttgcaag tttagtgttt aataaaccat taagtcaagt atttgcttgt ttagtgatta
      421 ctaaagataa ttaccacatg caaaaaaaag aaattcgccg tttacgtctc aaggagtggt
      481 ttaaagataa aactctgcca cccaaagaga agagctacct atctcaacta atgagtggga
      541 gagcctcgtt tggagaaaag gctgccagaa gaatagagca aacatacggg atgccggaag
      601 ggtatctgga tgcggaatac gcagaacaac cgggggtttc tccaccacat gcagggttaa
      661 cgtctaatca actggaatta ttgcagattt tttcagcctt ccctgaggat gagcaacgcc
      721 agataatcag cgagttaaag cagaaaaaag aatcaatgga agatctcata gcgagatgga
      781 ttgcggcgca aaaatgccgc cgcgcctgag ttataaaacc ggaggaaaca tgaatagagc
      841 cctttcacca atggtttctg aatttgaaac cattgaacaa gaaaacagtt acaacgaatg
      901 gctgcgtgcg aaagtagcaa cgagccttgc agatccgcgc ccagcaattc cccatgacga
      961 agttgagcgc agaatggcag aacgctttgc taagatgcgc aaggaacgga gcaagcagta
     1021 aaatgttacc cgtgttatgg cttgaaagcg cagataccga cctagatgat ataactagtt
     1081 atattgctcg tttcgacata gatgcggctg aacgcttatg gcagcgatta aggggttgtg
     1141 tgctgccgtt atccgaacat ccgtatttat acccaccaag cgacagagta cctggcttgc
     1201 gtgagattgt agcccaccct aactatataa ttctataccg cgtaacaaca tcaagcgttg
     1261 aagtagtaaa cgtgatccac gcaagacgcc agtttcccta actttcacta ccaatagaaa
     1321 cataacaacc gcaacgactt tatcaaaagc gttgtgtttg ttatgtcccg cggtttagtt
     1381 tttacttgac ttaagtttaa tgtttattaa actaaaaata ccaccccgcc ccacagaacg
     1441 cagggaaata cttcgagtta cccggcagtg gtcaggggtt aagtagccag cccgaggcgt
     1501 atgaacatga cggcagggtt caactttaat aactatgcag caggtttttg ttccgctacc
     1561 ccggcgttaa ggggaaatga ggtcagcatg gatactatcg atcttggcaa caacgcatct
     1621 ctggtgtacg gcgtgtttcc aaaccaggac ggcacgttca ccgcaatgac gtataccaaa
     1681 agcaaaacgt ttaaaaccga aaatggtgcc cgtcgctggc ttgccaaaaa taccagctaa
     1741 accatttatt ggattaattc aatattcttg ctgtaggggt atagccgag
//
LOCUS       DAZGJW010000385         1778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZGJW010000385 DAZGJW010000000
VERSION     DAZGJW010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.389"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(280..552)
                     /locus_tag="ACHKIK_005415"
     CDS             complement(280..552)
                     /locus_tag="ACHKIK_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086339.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            712..1245
                     /locus_tag="ACHKIK_005416"
     CDS             712..1245
                     /locus_tag="ACHKIK_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9086340.1"
                     /translation="MHEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1466..1579
                     /locus_tag="ACHKIK_005417"
     CDS             1466..1579
                     /locus_tag="ACHKIK_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086341.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            1581..>1778
                     /locus_tag="ACHKIK_005418"
     CDS             1581..>1778
                     /locus_tag="ACHKIK_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFO9086342.1"
                     /translation="MNRVLCVVIIVLLVACGALSLGLNHYRDHAIIYKEQRDKKASEL
                     ELANATITDMQIRQRDVAALDA"
BASE COUNT          469 a          348 c          482 g          479 t
ORIGIN      
        1 cgtaaggtgg cgcggggaga gtagtcgatg aataaacaat acgaactggt tgtaaaatga
       61 atatttctaa ctgaaaaaac gttccatgag gtaagaaaag gtcacaggca atcaataaca
      121 ggacgtgatg aaagaccctt gcatttgtgc gctttctctt tagatagcag cagatactga
      181 aaatctgagt tgtcggggag tcagggatac agctgtgcaa gagttggtca ttgtgattcc
      241 attgaaatcc tgtatgccat gaagggcagg attttatggc tacctgagct ttggtgatag
      301 taagttgaaa attcgcattt tttgctgaca tgcgtaacga gaatcccata agcagggagg
      361 acttaattct tcattaaccc atgcgttgat attatgtttc agccgttgaa gcatcagcgg
      421 tgttaatgtt gtggtaataa tatccagcgt tttatgtgag atcttaccgt aagggtctgc
      481 aagaatgctg cttgttgctt cgttattatc tgccatcaga agaagtaact ctgatttaac
      541 gttttctgtc attagttgta aaaatcttct gcgcaaactt tctttactgt tcatttatat
      601 ggcttcattt gttgtaatct gctgcgtctc aagggatatg tttatgagag cgaccatgag
      661 tgttggatta tatacctaac atatcaaggg attagaaatc gataaatccc catgcacgaa
      721 aaaataaaat acggcctgtc ggctgccgtt ctggcgctga ttggtgcagg tgcttctgcg
      781 cctgaaatcc tcgaccagtt tctggatgaa aaggaaggta accacaccac ggcataccgt
      841 gatggtgcgg ggatctggac catctgccgt ggtgccattc tggtggatgg taagcctgtt
      901 attcctggca tgaagctgtc aaaggaaaaa tgcgaccggg ttaatgccat cgaacgtgac
      961 aaggcgctgg catgggtgga gaaaaacatc cgggtgccgc tgaccgaacc ccagaaagcg
     1021 gggatcgcgt cattctgtcc gtacaacatt ggccccggta agtgcttccc gtcgacgttt
     1081 tataaacgaa ttaatgcagg cgatcgaaaa ggtgcctgtg aggcgattcg ctggtggatt
     1141 aaggacggtg gcagagactg ccgtatccgt tcaaataatt gctacggtca ggtctcacgg
     1201 cgtgaccagg agagcgcgct ggcgtgctgg gacatcgaca gatagcagaa tattttcctg
     1261 aaaaatgacg ttggccaacg cgggtggata acacgaaatc ctgaaaactg gtaaaaccta
     1321 agtgaataaa agtaaaaacc ccgtttgttg gcagcaagcg gggttttgtg ttttctgacc
     1381 ttgagcaagg caagggagaa attatgggta gggaggtact ttccctgtga ggaagtataa
     1441 aagattcttt ctgaggttgt ccattatgaa aggcattgaa gtggagacgc cagccagtct
     1501 ggatttaaca agagcggcag cttttgccat tcgtattgtg gccattgctg ttctggtctg
     1561 ggcaatccgt tggtggtgat atgaaccgtg ttctgtgcgt ggttatcatt gtcctgctgg
     1621 tggcctgtgg tgcgcttagt ctggggctga atcattaccg tgatcacgcc atcatctaca
     1681 aagagcagcg cgataaaaaa gccagtgagc tggagctggc gaacgcgaca attactgata
     1741 tgcagatacg ccagcgtgat gtcgctgcac ttgatgcc
//
LOCUS       DAZGJW010000386         1760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZGJW010000386 DAZGJW010000000
VERSION     DAZGJW010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.390"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..364)
                     /locus_tag="ACHKIK_005419"
     CDS             complement(<1..364)
                     /locus_tag="ACHKIK_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO9086343.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTSAPG
                     SDNLNGINVKYRYEFTDTLGLVTSFSYAGDRNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAY"
     gene            complement(432..>1760)
                     /locus_tag="ACHKIK_005420"
     CDS             complement(432..>1760)
                     /locus_tag="ACHKIK_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9086344.1"
                     /translation="QIELTPGYFQITAVPKLAVYDPTVQFEFWFSEAKIADTSQVETS
                     ARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEVSGQPSNDGEGYLEFFRE
                     KIGKLHLAQGLWELIDNSQLADEMAEMKTSITETRNEITQTVSKTLEDQSATIQQIQR
                     VQKDTNDDLAALYMLKVQKTKDGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPE
                     SGNSTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPAFSLTPDGRLTAKNADISGSV
                     NANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQ
                     KFDRQVMIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFS
                     SVIDMPAGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
BASE COUNT          384 a          501 c          416 g          459 t
ORIGIN      
        1 tgtaagccac gcccgccatc gcatacgcac tgaaccattc attcacgcgc acagagggcc
       61 ccgccatcac gctgaaccag cggttacgca cggaatcttc atgccagcgg gtatcgctgt
      121 aacgggtaag ctggcgattc ctgtctcctg catagctgaa tgacgtcacc agccccagtg
      181 tgtccgtaaa ctcataacgg tatttcacgt taatcccgtt aagattatcg ctaccgggag
      241 cgctcgtccg ggcatgaaga taccccgcgc tcagcgtggc ctgctgctca gacgcccatg
      301 caggcgcgcc ggatacggcc agacagatgg ctgcggacaa aatggcggca taaagtttac
      361 gcataattac ctctcgcttt tctgcaataa aaaaaggcgt catttctgac gcccgttctg
      421 ggttataaaa ttcagctgat actgatacct gctgtggatt ttttcatcac cacaaccagc
      481 agatcgctga tactggttgt tggtgtccag ttattcgctc ctgatgaaga tacggtgaat
      541 gtcagtgtca gcgtcccctg tccggcaggc atatctataa ctgaggaaaa tacgccctga
      601 gcatccgtcg tggactgatt aaaaatctcc tgaccattgc gggtcactct taaccggcag
      661 gttgaatacc agtatgactg ttggttatta ctgttgaaat tctcatgctt accaccgcgg
      721 aataacactg gcggtatcat gacctgccgg tcaaacttct gatcatcact gattcttacc
      781 gtgatggtgc cactggcata agtgctcgtg cgggggaaag acttgctgac cgttttgaca
      841 atatcgcctt caatctggtt ggctgatagt ttccccttaa tctgacagtt ctcattaatc
      901 gtgacgttgt tgagcgtccc tgagttcgca ttcacactgc cactgatatc cgcatttttc
      961 gccgtcagtc gcccgtccgg cgtcagggaa aatgccggag gattgccgga tgacgtgatg
     1021 ctcaccgcaa acagtcgttt caggaacacg tcgttcatga acagctgatt cccctgcgcc
     1081 acaaataacg gcgtgctgtt gccgctctcc ggatttatca tcgcgatacg gtcagccagc
     1141 agcagtatgt tgctcagtgg ctggccatca gtatcctcaa tccctgcacc aatcccggcc
     1201 acatagggaa tgccgtcttt cgttttttga accttcagca tgtacagcgc agccaggtca
     1261 tcatttgtgt ccttctgcac gcgctgtatc tgctgtatgg tggcgctctg gtcctccagc
     1321 gttttactga ccgtctgtgt gatttcattg cgggtttccg tgatgctggt cttcatctcc
     1381 gccatctcat ccgcaagctg gctgttatct atcagttccc acaacccctg agccagatgc
     1441 agttttccta ttttttcccg gaaaaattcc agataccctt caccatcatt gctgggctgc
     1501 ccgctgactt ccacaaacgc agatttcccc accaggttga cgctgcgcac gtaaaaccag
     1561 aaatccttcc cgggcttaat gtgcgggccg gatacactcc actgactgcc ggtccccaga
     1621 taacgggcag aggtttccac ctgagatgtg tctgcgattt ttgcctccga aaaccagaat
     1681 tcaaactgta ccgtcgggtc atatacagcc agtttcggga ccgccgttat ctgaaaatag
     1741 cccggtgtca gctcaatctg
//
LOCUS       DAZGJW010000387         1756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZGJW010000387 DAZGJW010000000
VERSION     DAZGJW010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.391"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..504
                     /locus_tag="ACHKIK_005421"
     CDS             <1..504
                     /locus_tag="ACHKIK_005421"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086345.1"
                     /translation="LSFSEFKQALRIRMCSHNDAEHKYEKQNQTAENFVLEPGETLFK
                     IPVTCPICGFTSEELDDSCNNQETTKYVEDDTECARRTIISTSPNSRTNKSHYERVIN
                     PLPQTNKKDAGGQKTQWNNGISVKKRRSIKDKQRSANNCKRFIPVINFHVLKRIYWWL
                     RHCLMNP"
     gene            991..1179
                     /gene="dicB"
                     /locus_tag="ACHKIK_005422"
                     /gene_synonym="ftsT"
     CDS             991..1179
                     /gene="dicB"
                     /locus_tag="ACHKIK_005422"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO9086346.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            1176..1367
                     /gene="ydfD"
                     /locus_tag="ACHKIK_005423"
     CDS             1176..1367
                     /gene="ydfD"
                     /locus_tag="ACHKIK_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HFO9086347.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            1461..>1756
                     /locus_tag="ACHKIK_005424"
     CDS             1461..>1756
                     /locus_tag="ACHKIK_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
                     /protein_id="HFO9086348.1"
                     /translation="MSKVFICAAIPDELATREEGSVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETES"
BASE COUNT          513 a          388 c          398 g          457 t
ORIGIN      
        1 ctcagcttca gtgagtttaa acaggcactc agaataagaa tgtgctctca caatgacgcg
       61 gagcacaaat atgagaagca aaatcagacc gcagaaaatt ttgttctgga acccggagaa
      121 acgcttttca aaattcccgt tacgtgcccc atttgcggtt ttacatcaga agaacttgac
      181 gactcctgta acaatcagga aacaaccaag tatgtcgaag atgataccga gtgcgcacga
      241 agaacgatta tatccacgag tccaaactcc aggaccaata aatctcacta tgagagggtg
      301 attaatccac tcccccaaac caataaaaaa gatgccggag gacaaaaaac ccaatggaac
      361 aacgggatat ctgtcaaaaa aagacgttcc attaaagaca aacaacgcag cgccaacaac
      421 tgtaagcgct ttataccagt aatcaatttt catgttctta agcggattta ttggtggttg
      481 cgacattgct taatgaatcc ttaaaactgt ggtgatttta aggataccac ctcgcctgac
      541 gtggttaaaa gcaggcacac aacacgaaag cgcacggcga agttcgtctc actgtacggt
      601 gtcgttaaat ttaattcgac cgtgcgcttc cggttgtggc aacccgcaaa atggcgcggc
      661 ggtaagtatg gcggggttat tccttccccg ttgaggacac cgggttgtca ggttgaccat
      721 acgcttaagt gacaaccccg ctgcaacgcc ctctgttatc aattttctgg tgacgtttgg
      781 cggtatcagt tttactccgt gactgctctg ccgccctttt taaagtgaat tttgtgatgc
      841 ggtgaatacg gctaagcgca cgcggaacag ttaaaaccaa aaacagtgtt atgggtggat
      901 tctctgtatc cgacgttaat tgttaactgg ttaacgtcac ctggaggcac caggcactgc
      961 atcacaaaat tcattgttga ggacgcgata atgaaaacgt tattaccaaa cgttaatacg
     1021 tctgaaggtt gttttgaaat tggtgtcact atcagtaacc ctgtatttac tgaagatgcc
     1081 attaacaaga gaaaacaaga acgggagcta ttaaataaaa tatgcattgt ttcaatgctg
     1141 gctcgtttac gtctgatgcc aaaaggatgt gcacaatgaa ttcagcattt gcgcttgttc
     1201 tgacagtttt tcttgtttcc ggagtgccag ttgatattgc agtcagtgtt cacaggacaa
     1261 tgcaggagtg tatgactgca gcaaccgaac agaaaattcc cggtaactgt tacccggtcg
     1321 ataaagttat tcaccaggat aatatcgaaa tcccggcagg tctttaaaac agttccgtaa
     1381 taaatatccg gtttcattct tatatgccag caatggcagg gatttgttca tccttaaatc
     1441 tgtcatgagg ttaaaacaaa atgagtaaag tctttatttg cgccgctatt cctgacgaac
     1501 tggcaacaag ggaagaaggc tctgtggctg tagccacagc cattgaagct ggcgacgaac
     1561 gccgtgctcg agcaaaattt cactggcagt tcctggaaca ttatccggct gctcaggact
     1621 gcgcttataa atttattgtc tgcgaggata aacctggcat accccgccct gccctcgatt
     1681 catgggatgc tgaatatatg caggaaaacc gctgggatga ggagtctgct tcttttgtcc
     1741 cggttgagac tgaatc
//
LOCUS       DAZGJW010000388         1756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZGJW010000388 DAZGJW010000000
VERSION     DAZGJW010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.392"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(398..580)
                     /locus_tag="ACHKIK_005425"
     CDS             complement(398..580)
                     /locus_tag="ACHKIK_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO9086349.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRYRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            complement(577..1104)
                     /locus_tag="ACHKIK_005426"
     CDS             complement(577..1104)
                     /locus_tag="ACHKIK_005426"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO9086350.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            complement(1101..1547)
                     /locus_tag="ACHKIK_005427"
     CDS             complement(1101..1547)
                     /locus_tag="ACHKIK_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO9086351.1"
                     /translation="MKQIYMLRNEAIRNNAIDAILSLPIDDKSPHEVHIKEPKRSNPQ
                     NRLMWALLQDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSVPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGRAA"
     gene            complement(1555..>1756)
                     /locus_tag="ACHKIK_005428"
     CDS             complement(1555..>1756)
                     /locus_tag="ACHKIK_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086352.1"
                     /translation="ELIFSALRILGAMWMVATFIVVASSFVRLVGEGKDLVGVLFGSI
                     FLWVIIGVAPVAVAKMAWRFVS"
BASE COUNT          450 a          479 c          351 g          476 t
ORIGIN      
        1 cttaatgtat ttctataaaa gcaaattaca aactcacaac aaaaaacctc ataacacatt
       61 aacaatcaat tcttttcatc tttataaatt ctcatatcag gcttgcaccc gataaaccga
      121 cgaaaactat ttaaaaccca tcgagtgaag taatctctaa aaccaaagaa ataccaagtg
      181 aaaatattca cgataaaatg cccggtcaaa gcccctcctg taccgcatgc aagaacagta
      241 aaaaatcaga tgttttcata aatatcagtc ctcatcgttt tgcctggcat gtcctttacc
      301 agcaatcttc tgtatgcact aagcctagat agaatccact cagtgtacac tgaagcccgc
      361 tcgacgcttt cttgttcgta acttcgattt tagtcaatta ccttgttttc ctcgcacgat
      421 gtcttagcca ccggatatcc cacaggtgag ccgtgtaatt gaaggttttt acgtcagatt
      481 cttttgggat tggcttgcgt ttatttctgt agcgtttcgt tggaaggtat ttgcagtttt
      541 cgcagatgat gtcggtgaaa cttcgtcgct gtcgcctcat gccgccctcc tgacgccctg
      601 cccgatcgcc atcaatgccg ctttggatac ggtagtaaac atccgtcgag gactgatgaa
      661 cggtcgccaa atcagcagca tggagccttt actgtttccc ttcttctcca gccctgtcga
      721 tggttcgata aaattaatcc gtccatcagt gataatgcga acttcgtcaa cactctccag
      781 agccttgctg aaccatccga cagacatatc ctctggcaca agcatcacta ccgtctgtcg
      841 ctgttgtatg cactgctcag cggctttttc cacccacggc ctgatattgc tgtacggtgg
      901 gttattccag attgcaccgt ggcttatcca ctcagaattg agtgcgtcgt cggcctcagt
      961 tagccagtga gcgcacagag catttttgtc gctcgcagct gaatccagcc agaatccaaa
     1021 ctcaatatcc agcgcatcaa aaagccaaag cggcgtttgc cagcagtcct tgtcgtgtgc
     1081 tggtgtattt gatttgatag tcatgcagcc ctaccttttc gttgtgacca ttcatactct
     1141 cgccgggagt catcactcca ccgcacgttg cgctctgagc cgaaccaaaa catgatttcg
     1201 ataagctcag tcatgctggc ctttcgcatt ttgctggtac gcacgccaag catgacaacg
     1261 ccaccatcga taccaggcac acttcgttgc tccagttttt tggtcttaag ccacagggca
     1321 gtgaacaggt ctttccagtc ctccggcgca agtctctgtc catgccaaag cacctgacgt
     1381 gatacgtcct gcaataacgc ccacataagg cggttttgag gattgctccg ctttggttct
     1441 ttaatgtgga cttcgtgagg tgacttgtcg tcgatcggaa gtgagagtat tgcgtctatg
     1501 gcgttgtttc tgattgcttc gttgcgaagc atgtatattt gcttcattgt cacctcaact
     1561 cacaaaacgc cacgccattt ttgctacagc gacaggcgca acaccgataa tcacccacag
     1621 gaaaatgcta ccgaaaagca caccaaccag gtctttacct tcgcctacca gccggacaaa
     1681 actgctggca accacaatga acgtcgccac catccacata gcaccgagaa tcctcaatgc
     1741 agaaaaaatc aactca
//
LOCUS       DAZGJW010000389         1752 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZGJW010000389 DAZGJW010000000
VERSION     DAZGJW010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.393"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..621
                     /gene="traT"
                     /locus_tag="ACHKIK_005429"
     CDS             <1..621
                     /gene="traT"
                     /locus_tag="ACHKIK_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO9086353.1"
                     /translation="QYTSTIWLEPASERTVFLQIKNTSDKDMSGLQGKIADAVKARGY
                     QIMTSPDKAYYWIQANVLKADKMDLRESQGWLSRGYEGAAVGAALGGGITAYNTGSAG
                     TTLGVGLATGLIGMAADAMVEDINYTMITDVQIAERTRTSVRTDNVAALRQGTSGSKI
                     QTSTETGNQHKYQTRVVSSANQVNLKFEEAKPHLEDQLAKSIANIL"
     gene            complement(669..920)
                     /locus_tag="ACHKIK_005430"
     CDS             complement(669..920)
                     /locus_tag="ACHKIK_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO9086354.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(944..1234)
                     /locus_tag="ACHKIK_005431"
     CDS             complement(944..1234)
                     /locus_tag="ACHKIK_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO9086355.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
BASE COUNT          528 a          420 c          365 g          439 t
ORIGIN      
        1 cagtacacct caaccatctg gcttgaacct gcgagcgaac gcaccgtttt cctgcagatc
       61 aaaaacacgt cggataaaga catgagtgga ctacagggaa aaattgccga tgcagtcaaa
      121 gccagaggtt atcagatcat gacttccccg gataaagctt actactggat ccaggctaac
      181 gttctcaaag cagacaaaat ggatctgcgt gaatctcagg gatggctgag tcgcggttat
      241 gaaggtgcag cagtcggagc tgctcttgga ggtggaatca cagcgtacaa taccgggtcg
      301 gcaggtacca ctctcggtgt tggactggct accggtctga ttggcatggc cgcggatgca
      361 atggtggaag atataaatta cacaatgatt acagatgttc agattgccga gcgaaccaga
      421 accagcgtca gaacggataa tgttgcagct ttgcgtcagg gaacatcagg ctccaaaatt
      481 cagacaagca cagaaaccgg caaccagcat aaataccaga cccgggttgt atccagtgcc
      541 aaccaggtta atctgaagtt tgaagaagct aaacctcatc tggaagacca gctggcaaaa
      601 tcaatcgcca atatactctg atataaatat cccggtggcc attcacctgg cctccggccc
      661 tcttagtctc aacgttcaga caattcagat attattttcc ctgtcacacc atatacccat
      721 gccggaacag aatctaatga cggtaccagt aaattcttca gcgttcggtt tgcctgacgc
      781 ccgtagctgt atttgtgtct gtttcttctt atacaagagc agtagtacaa tttctcttcc
      841 agagtcagtt caactttagg acgtaaccaa taaagatctc ttccactata aaatggtgaa
      901 tcctgtacaa acgattccat aacagaacct cctgctgcta cagttaacac agctgctggc
      961 atggactaat accatctttt aatttaaccc tggcggagac gccattattt ttggacgttc
     1021 ttgaaacaaa attaattcct gaagaatctt tctccagttt attcagctca agattcagtc
     1081 cataattaac gtcaaataac tctgataaag gtatcagttt atgttcagga taatgattat
     1141 cccgacgaca acaaatatta aaatcctgca tattacccga catcaggagt tcaaaaccgt
     1201 ccccggcatc attcatactg gcagataaat tcattaccca gtttagtgct aatcaacagg
     1261 tacggcttaa cttacaggaa ttccccggga ggggcggact catcctcagt tagcggcaaa
     1321 atcgcatcac agccaatcgc ctgtctcgat tccccatcgg cgaacaatgt atgtggccac
     1381 aaaatcctgg ctggctcaga acgaagagca tctgagcaaa gaggtcatca atctggtatg
     1441 aatatctggc agaaaatatc ccctgaatcc acaactgctg tgcatatctg ctcaaatcag
     1501 catcaatcat aaaccagtaa tcacagtgcc tgtgattggg ttacaggaat aagtccccct
     1561 tcataaggca tatcatcaat accggtgaag gaaaagaaag catcagatgg cctgatagtc
     1621 tgcgtcagtc aatgacatca gtgctttatc cggaacaatg tatccatgtt ggcgacagag
     1681 aaagccattg ccatgaacag ttttgtttat ccccggaaag tcatttctgt tacaaattta
     1741 tattgacaaa ct
//
LOCUS       DAZGJW010000390         1744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZGJW010000390 DAZGJW010000000
VERSION     DAZGJW010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.394"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1744
                     /locus_tag="ACHKIK_005432"
     CDS             <1..>1744
                     /locus_tag="ACHKIK_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO9086356.1"
                     /translation="GQGNFHIHDATMLPDMKKAQYKQRYQQELILRGNYASATDQLRV
                     YILKEHGGIYTDYDVTPAYGKNIYKIIQEHSINYDFLEKEHHRRALNDEILSVISKEP
                     SSGIKNKLPEQDRKRLSVIVDKIKKLDPKDIFSPIDTMIIRDSMVMSKRYQYWGKEKG
                     WNIRANNNFLATHKGSKVTDFVINRQEIAYKRLLEVREKLRDTGNIAQQHSDNINSLD
                     YHRGDKKAEANLVVSWVKHAANKEKNKEVLKDLKKDLATYGALLDNNVNGLKPKDIRA
                     TEDFLRGYSSGSAERIVDGFHSDMNIKKLVKLMKKNINSLSNEQKGAMAYEIEKRALL
                     ATFHSNVEEYHKLFNKVVSKGSTDKYAVESLIPQLFFLNLSGDGYGGRCDPLSILVLT
                     SKYLENQSSQKQTKLFLENLYSTAAVLSEPSLYSEVEIANANKLLTSLAKLHAKNPLS
                     STLTQIWKEKIVHQTVEQVIDLIISPKATGNPVLLKLEAPGHAMAAWAIGNDNERKYG
                     FYDANGGVVEFSDTEKFKEYMTEFFSASGLNKAEKYHLKKLPPHNYPVFDQIVLLDGE
                     KLSQYKTNYNDDAIK"
BASE COUNT          663 a          263 c          334 g          484 t
ORIGIN      
        1 ggtcaaggta attttcatat tcatgatgca acaatgctgc cagatatgaa aaaagcacaa
       61 tataaacaac gatatcaaca agaattgata ttgcgtggca attatgcatc tgctacagat
      121 caattacgtg tttatatact caaagaacat ggtggaatat atacagatta tgacgtcact
      181 cctgcttatg gaaaaaacat ctacaaaata attcaggagc atagtattaa ttatgatttc
      241 ttagagaaag agcaccaccg aagggcatta aatgatgaga ttctttctgt tataagtaaa
      301 gagccaagct caggaataaa aaataaactc cctgaacaag atagaaaacg tctctctgtt
      361 atcgtcgaca agataaaaaa acttgaccct aaagatatat tttctcctat cgacactatg
      421 ataatcagag actcaatggt gatgtcaaaa cgttatcaat attggggaaa ggagaaaggc
      481 tggaatattc gtgcaaataa taattttctt gccacacata aaggaagtaa agtaactgat
      541 ttcgtaataa acagacaaga aatagcttat aaaagacttc ttgaggtaag agagaagtta
      601 cgagatacag gcaacatagc acaacaacat tcagataata ttaattctct tgattatcat
      661 cgaggagata aaaaggcaga agcaaatttg gtggtatcgt gggtgaagca tgccgcgaac
      721 aaagagaaaa ataaagaggt tcttaaagac ttaaaaaaag acttggcaac atatggagca
      781 ctacttgata acaatgtaaa tggattaaaa cctaaagata ttagagcaac agaggatttc
      841 ttaagaggct atagttcagg aagtgcagag aggatagtgg atggattcca tagtgacatg
      901 aacattaaaa agttagttaa gctgatgaaa aaaaatataa atagcttaag taacgagcaa
      961 aaaggagcta tggcctatga gattgaaaaa cgtgcgttat tggctacatt ccattctaat
     1021 gtagaagagt atcataagct tttcaataaa gtcgtatcaa aaggttctac ggataaatat
     1081 gctgttgaat ccttgatacc acaattattt ttcttaaacc tttcaggtga cggatatgga
     1141 ggtcgttgtg atccgttgtc tattttggtt ctcacttcaa aataccttga aaaccaatct
     1201 tcacagaagc aaacaaagct atttctggaa aatctatatt caacagcagc ggtattaagt
     1261 gagcctagtt tatatagtga agtagaaata gcgaacgcca acaaactgtt aacaagcttg
     1321 gctaaacttc atgcaaaaaa tccgttgagc tctaccctaa cacaaatatg gaaagagaaa
     1381 atagtacatc aaacagttga gcaagttata gatttaataa tctccccaaa agctacaggt
     1441 aatccagttt tattgaaact ggaggctcct ggtcatgcaa tggctgcttg ggctattggt
     1501 aacgataatg agcgaaaata tggtttttat gatgcaaatg gtggtgttgt tgagttttca
     1561 gatacagaaa agtttaaaga atatatgaca gaattttttt ctgcttcagg attgaataaa
     1621 gctgaaaaat atcatttgaa aaagcttcca cctcataatt atcctgtctt cgatcagatt
     1681 gttttattgg atggtgaaaa attatcgcaa tataaaacga attataatga tgatgccata
     1741 aaga
//
LOCUS       DAZGJW010000391         1729 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZGJW010000391 DAZGJW010000000
VERSION     DAZGJW010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.395"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1285
                     /gene="rhsB"
                     /locus_tag="ACHKIK_005433"
     CDS             <1..1285
                     /gene="rhsB"
                     /locus_tag="ACHKIK_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO9086357.1"
                     /translation="EPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPEVTWYG
                     WDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSLADTLQQSGGEDG
                     GSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIH
                     LYHCDHRGLPLALISKEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGL
                     YYNRHRYYDPLQGRYITQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTN
                     GLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPN
                     RGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIKSF
                     YLGTPSGNFKAIDNHGKEITNRKGLPNVCRVHGNM"
     gene            1257..1640
                     /gene="yhhH"
                     /locus_tag="ACHKIK_005434"
     CDS             1257..1640
                     /gene="yhhH"
                     /locus_tag="ACHKIK_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HFO9086358.1"
                     /translation="MSAEFMVICKKLLFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYE
                     SEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSD
                     QIAGGVFIIEIGKNDGRILNFGHGK"
BASE COUNT          529 a          349 c          462 g          389 t
ORIGIN      
        1 agagccgctg gtcgaaagtc gctatcttta cgacccgctg ggccgcaggg tggcaaaacg
       61 ggtatggcga cgtgaacggg acctgacggg ctggatgtcg ctgtcacgga aaccggaagt
      121 gacctggtac ggctgggacg gcgaccggct gaccacgata cagaacgaca gaacccgcat
      181 ccagacgatt tatcagccgg ggagcttcac gccactcatc agagttgaaa ccgccaccgg
      241 tgagctggcg aaaacgcagc gccgcagcct ggcggatacc cttcagcagt ccggcggcga
      301 agacggtggc agtgtggtgt tcccgccggt gctggtgcag atgctcgacc ggctggaaag
      361 tgaaatcctg gctgaccggg tgagtgagga aagccgccgc tggctggcat cgtgcggcct
      421 gactgtggag cagatgcaaa accagatgga cccggtgtac acgccggcgc gaaaaatcca
      481 cctgtaccac tgcgaccatc gcggcctgcc gctggccctt atcagtaagg aaggggcaac
      541 agaatggtgc gcagaatacg atgagtgggg caacctgctg aatgaagaga acccgcatca
      601 gctgcagcag cttatccgcc tgccggggca gcagtatgat gaggagtccg gcctgtatta
      661 caaccgccac cgctattatg acccgctgca ggggcgatat atcactcagg atccgattgg
      721 actgaagggg ggatggaacc tgtatggata tcaattgaat ccgatatcag acatcgaccc
      781 cctgggttta tctatgtggg aggatgcaaa atcgggggca tgtactaatg gtctttgcgg
      841 cacactatcc gctatgatag gtccagataa atttgattct atagatagca ccgcatatga
      901 cgccttaaat aaaataaata gccaatctat ttgcgaagat aaagagttcg ctggtttaat
      961 atgtaaggat aatagtggca gatatttctc aacagcacct aaccgaggag aaagaaaagg
     1021 atcatatcca ttcaatagcc cttgccctaa tggtactgag aaagtatcag cttatcatac
     1081 tcatggtgca gatagtcatg gagaatattg ggacgaaata ttttcaggta aagatgagaa
     1141 aatagttaaa agtaaagata acaatatcaa gtcattttat ttaggtacgc ccagtggtaa
     1201 ttttaaagca atagataacc acgggaagga aataacaaac agaaaaggat tacctaatgt
     1261 ctgcagagtt catggtaata tgtaaaaaac tattgtttag gaactgtgtc attgtatctt
     1321 tgtttgtttt tacatacaac acatgggcgc agtgtaataa taatattaaa ataatgcgca
     1381 agtatgaaag tgaaggtaaa tataccgtta gaaatttggt taaaaataaa gctatagcat
     1441 tggaattagc tgagatatat gttaagaatc gttatggaca ggatgccgca gaagaagaaa
     1501 aaccatacga aatcactgag ttaacaacaa gttgggttgt tgaaggtacc attcactcag
     1561 accaaattgc tggtggggtt tttattatag aaataggcaa aaatgatggg agaattctga
     1621 attttggcca cggaaaataa gtatgtaagg ataatcaaac atcgtgtgcg ctgatggcag
     1681 agtaggtgga ggactccaga cagtcaaacg atagaaaaag atagccttt
//
LOCUS       DAZGJW010000392         1726 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZGJW010000392 DAZGJW010000000
VERSION     DAZGJW010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.396"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..166
                     /gene="xisR"
                     /locus_tag="ACHKIK_005435"
     CDS             <1..166
                     /gene="xisR"
                     /locus_tag="ACHKIK_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO9086359.1"
                     /translation="LKKAREKSFMEGREYKHVAHDGMPWDNSPCFYNLEEIDRWIERQ
                     ASARPRRHLT"
     gene            201..1481
                     /locus_tag="ACHKIK_005436"
     CDS             201..1481
                     /locus_tag="ACHKIK_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9086360.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTSKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSKFAPTMPHNEAMKN"
BASE COUNT          567 a          346 c          374 g          439 t
ORIGIN      
        1 gttaaaaaag gcccgggaaa aatcgtttat ggaaggaagg gaatataagc atgtcgctca
       61 tgacggtatg ccatgggata acagtccatg cttttataac ctggaagaaa ttgatcgctg
      121 gattgagcgc caggcatcag cgagaccaag acgtcatctt acttgactaa aagccacact
      181 aactaatgag agaagttgaa atgaaatatc cgacaggcgt ggaaaaccat ggagggaaat
      241 tacgtatctg gtttgtttat aaaggcgtaa gagtcaggga aaatctgggg gttcctgaca
      301 catcaaaaaa caggcgcgtt gcaggtgaac tacgctcctc tgtttgttac gcaataaaaa
      361 ctggtgtttt cgactatgca aaacagtttc cctcctcacg caatctggaa aaatttggtg
      421 aggcccgaca agatttaacc ataaaagaac tggctgaaaa atttttggca ctgaaagaaa
      481 ctgaagtcgc caaaacatca ctcaacacat accgtgccgt catcaaaaat atcctgagca
      541 taatcggtga aaaaaatctt gcctcatcga ttaataaaga aaaattactg gaggttcgta
      601 aagagctact gactggatac cagatcccca aaagtaacta tattgttaca caaccaggga
      661 gatcggctgt aactgtaaat aattacatga caaatcttaa cgccgtgttc cagtttggtg
      721 ttgataacgg ttacctggca gataatccgt ttaaggggat ctcgccatta aaggaatcaa
      781 gaaccattcc ggatcctctt tcgcgggagg aatttatccg tcttatcgat gcgtgcagaa
      841 atcagcaagc aaaaaattta tggtgtgttt ctgtttatac tggcgttcgc cctggtgagc
      901 tgtgtgcact tggatgggag gacatagatc tgaaaaatgg aacaatgatg atcaggagaa
      961 atttagcaaa agaccgtttc acggtaccaa aaacacaggc gggaaccaat cgggtcattc
     1021 atcttattaa gccagcaatc gacgctctcc ggagtcagat gacattaacg agactgagca
     1081 aagagcatat cattgatgtt cacctcagag agtatggcag aacagaaaaa caaaaatgca
     1141 cctttgtttt tcaacctgaa gtgtcagcga gagtaaaaaa ttatggtgac cattttaccg
     1201 ttgactcaat aaggcagatg tgggacgcag cgataaaacg tgccggactc cgccatcgaa
     1261 aatcatatca gtcgagacat acttatgcct gctggtcgct gacagctggt gctaacccgg
     1321 catttatagc aaaccagatg ggccatgcag atgcgcaaat ggtatttcag gtatacggaa
     1381 aatggatgtc tgaaaacaat aatgcacagg tagctttgtt aaatacacag ttaagcaagt
     1441 ttgccccaac catgccccat aacgaagcaa tgaaaaatta atttaatatt tatcaaatag
     1501 ttaacacgca tgactcttga aatccataaa ttcaagcgca gtgcccagcc atcccgatac
     1561 tgctgctttc accaaatcct tagtgcttct ttcatgtttt tctattgtca taatggttat
     1621 ctctaaaaaa gaggtaagat gcgtactact tactcgccgt tattggtatt attcagaaaa
     1681 agtgagcaag actttgcagc aatgtttttg atcctgttca aataaa
//
LOCUS       DAZGJW010000393         1719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZGJW010000393 DAZGJW010000000
VERSION     DAZGJW010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.397"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1230
                     /locus_tag="ACHKIK_005437"
     CDS             <1..1230
                     /locus_tag="ACHKIK_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO9086361.1"
                     /translation="RYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRL
                     TTIQNDRTRIQTIYQPGSFTPLIRVETATGEQAKTQRRSLADTLQQSGGEDGGSVVFP
                     PVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDH
                     RGLPLALVSTEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHR
                     YYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKP
                     PTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCT
                     PQDDSQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDL
                     GEILAKMKQRTSRGIRK"
     gene            1227..>1719
                     /gene="ybfC"
                     /locus_tag="ACHKIK_005438"
     CDS             1227..>1719
                     /gene="ybfC"
                     /locus_tag="ACHKIK_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO9086362.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTA"
BASE COUNT          497 a          390 c          455 g          377 t
ORIGIN      
        1 cgctatcttt acgacccgct gggccgcagg gtggcaaaac gggtgtggcg acgtgaacgg
       61 gacctgacgg gctggatgtc gctgtcacgg aaaccgcaag tgacctggta cggctgggac
      121 ggcgaccgcc tgaccacaat acagaacgac agaacccgca tccagacgat ttatcagccg
      181 gggagcttca cgccactcat cagggttgaa accgccaccg gtgagcaggc gaaaacgcag
      241 cgccgcagcc tggcggatac ccttcagcag tccggcggcg aagacggtgg cagtgtggtg
      301 ttcccgccgg tgctggtgca gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg
      361 gtgagtgagg aaagccgccg ctggctggca tcgtgcggcc tgacggtgga gcagatgcaa
      421 aaccagatgg acccggtgta cacgccggcg cgaaaaatcc acctgtacca ctgcgaccat
      481 cgcggcctgc cgctggcgct tgtcagcacg gaaggggcaa cagaatggtg cgcagaatac
      541 gatgaatggg gcaacctgct gaatgaagag aacccgcatc agctgcagca gcttatccgc
      601 ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca ccgctattat
      661 gacccgctgc aggggaggta tatcactcag gatccgattg ggctgaaggg gggatggaat
      721 ttttatcagt atccgctgaa tccggttcag tatatagatt caatgggact ggcatcaaaa
      781 tatggacact taaataatgg cggatatgga gcgagaccca acaaaccgcc tacgcccgat
      841 ccaagtaaat tgccggacat agcgaaacaa ttaagactgc catatcctat tgaccaggcc
      901 agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta cgactacaca
      961 ctgtattgca ggaagtgggt aaaaccaaat ctgacttgta cgccacagga tgattcccag
     1021 tatccaggga tggatacaaa gacagcaagt gattacctgc cacagacaaa ttggccaaca
     1081 actcaattac caccaggata tacttgtgca gaaccctatt tattcccaga cattaataaa
     1141 cccgatgggc cagcaacagc agggatagat gatttgggtg aaattttagc taagatgaaa
     1201 cagagaacat cgagaggaat aagaaaatga aaagagtttt gttctttttg ctgatgatat
     1261 ttgttagttt tggtgttata gctgattgcg aaatacaagc taaagatcat gattgtttta
     1321 ctattttcgc taagggtact attttttccg cgtttcctgt cttaaataat aaagccatgt
     1381 ggagatggta tcagaatgag gacatcggtg agtattattg gcaaacagaa ctgggtacat
     1441 gtaaaaataa taaattcaca ccaagcgggg cgagactctt aattcgcgtc gggtcactac
     1501 gccttaatga aaatcatgcc atcaaaggaa cgttgcagga gttgataaat acagcagaaa
     1561 aaacggcgtt tctcggcgat cggtttagaa gttatataag agcgggtatc tatcaaaaaa
     1621 agagcagtga tccagttcag ttgttggcag tccttgataa ttcaatcatg gtaaaatatt
     1681 ttaaagatga gaaaccaact tatgctcgaa tgacagcac
//
LOCUS       DAZGJW010000394         1711 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZGJW010000394 DAZGJW010000000
VERSION     DAZGJW010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.398"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            59..166
                     /locus_tag="ACHKIK_005439"
     CDS             59..166
                     /locus_tag="ACHKIK_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086363.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            372..1655
                     /gene="gltA"
                     /locus_tag="ACHKIK_005440"
     CDS             372..1655
                     /gene="gltA"
                     /locus_tag="ACHKIK_005440"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO9086364.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT          447 a          412 c          401 g          451 t
ORIGIN      
        1 atctaatttg acaatcattc aacaaagttg ttacaaacat taccaggaaa agcatataat
       61 gcgtaaaagt tatgaagtcg gtatttcacc taagattaac ttatgtaaca gtgtggaagt
      121 attgaccaat tcattcggga cagttattag tggtagacaa gtttaataat tcggattgct
      181 aagtacttga ttcgccattt attcgtcatc aatggatcct ttacctgcaa gcgcccagag
      241 ctctgtaccc aggttttccc ctctttcaca gagcggcgag ccaaataaca aacgggtaaa
      301 gccaggttga tgtgcgaagg caaatttaag ttccggcagt cttacgtaat aaggcgctaa
      361 ggagacctta aatggctgat acaaaagcaa aactcaccct caacggggac acagctgttg
      421 aactggatgt gctgaaaggc acgctgggtc aagatgttat tgatatccgt actctcggtt
      481 caaaaggtgt gttcaccttt gacccaggct tcacttcaac cgcatcctgc gaatctaaaa
      541 ttacttttat tgatggtgat gaaggtattt tgctgcaccg cggtttcccg atcgatcagc
      601 tggcgaccga ttctaactac ctggaagttt gttacatcct gctgaatggt gaaaaaccga
      661 ctcaggaaca gtatgacgaa tttaaaacta cggtgacccg tcataccatg atccacgagc
      721 agattacccg tctgttccac gctttccgtc gcgactcaca tccaatggca gtcatgtgtg
      781 gtattaccgg cgcgctggcg gcgttctatc acgactcgct ggatgttaac aatcctcgtc
      841 atcgtgaaat tgccgcgttc cgcctgctgt cgaaaatgcc gaccatggcc gcgatgtgtt
      901 acaagtattc cattggtcag ccatttgttt atccgcgcaa cgatctctcc tatgccggta
      961 acttcctgaa tatgatgttc tccacgccgt gcgaaccgta tgaagttaat ccgattctgg
     1021 aacgtgctat ggaccgtatt ctgatcctgc acgctgacca tgaacagaac gcctctacct
     1081 ccaccgtgcg taccgctggc tcttcgggtg cgaacccgtt tgcctgtatc gcagcaggta
     1141 ttgcttcact gtggggacct gcgcacggtg gtgctaacga agcggcgctg aaaatgctgg
     1201 aagaaattag ctccgttaaa cacattccgg aatttgttcg tcgtgcgaaa gataaaaatg
     1261 attctttccg cctgatgggc ttcggtcacc gcgtgtacaa aaattacgac ccgcgcgcca
     1321 ccgtaatgcg tgaaacctgc catgaagttc tgaaagagct gggcaccaaa gatgacctgc
     1381 tggaagtggc tatggagctg gaaaacatcg cgctgaacga cccgtacttt atcgagaaga
     1441 aactgtaccc gaacgtcgat ttctactctg gtatcatcct gaaagcgatg ggtattccgt
     1501 cttccatgtt caccgtcatt ttcgcaatgg cacgtaccgt tggctggatc gcccactgga
     1561 gcgaaatgca cagtgacggt atgaagattg cccgtccgcg tcagctgtat acaggatatg
     1621 aaaaacgcga ctttaaaagc gatatcaagc gttaatggtt gattgctaag ttgtaaatat
     1681 tttaacccgc cgttcaaatg gcgggttaat t
//
LOCUS       DAZGJW010000395         1705 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZGJW010000395 DAZGJW010000000
VERSION     DAZGJW010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.399"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            25..849
                     /gene="rcnA"
                     /locus_tag="ACHKIK_005441"
     CDS             25..849
                     /gene="rcnA"
                     /locus_tag="ACHKIK_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO9086365.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVVSKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWHGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            1068..1406
                     /gene="rcnB"
                     /locus_tag="ACHKIK_005442"
     CDS             1068..1406
                     /gene="rcnB"
                     /locus_tag="ACHKIK_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO9086366.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1488..>1705)
                     /locus_tag="ACHKIK_005443"
     CDS             complement(1488..>1705)
                     /locus_tag="ACHKIK_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9086367.1"
                     /translation="FILESNGSSVTLLNITNSSKRYTNLKEIAAKSKLTDTTVSIPIT
                     ASYYVYDTNKVKSGALEATALINVKYD"
BASE COUNT          424 a          400 c          399 g          482 t
ORIGIN      
        1 gaattaatac taagaattat tatcatgacc gaatttacaa ctcttcttca gcaaggaaac
       61 gcctggttct tcatccccag cgccatctta cttggtgcgc ttcatggcct ggaaccaggg
      121 cactcaaaaa cgatgatggc ggcgtttatc atcgccatca aaggcaccat taaacaggcg
      181 gtgatgctcg gactggcagc aactatttcg cataccgcag tggtctggtt gattgccttt
      241 ggcgggatgg tggtcagcaa gcgctttact gctcaatcag cagaaccgtg gctccagttg
      301 atttccgcag tgatcattat tagcaccgcg ttctggatgt tctggcgtac ctggcacggc
      361 gagcgcaact ggctggagaa tatgcacgag catgatcatg agcatcatca tcacgatcac
      421 gaagatcacc acgaccatgg acatcatcac catcacgaac atggcgagta tcaggatgct
      481 catgcacgag cccatgctaa tgacattaaa cgacgctttg atggtagaga ggtcaccaac
      541 tggcaaattt tgttatttgg cttaaccggt ggccttatcc cctgcccggc agcaattacc
      601 gtgctgttga tatgcattca gttgaaagcc ctgacactgg gcgcaacact ggtcgtcagt
      661 ttcagcattg gcctggcgtt aacgcttgtc accgtaggcg ttggcgcagc aatcagcgtt
      721 cagcaggtcg caaaacgctg gagcggattt aacactctcg ctaaacgcgc cccctatttt
      781 tccagtctgt tgattggctt agtcggtgtg tatatgggcg tacatggctt catgggcata
      841 atgcgataac tcaatccttt aggcttgcat gttatcttca gcccaggatg attcttaaat
      901 cagctatttc cgctgactct ctgctcgcta aggatgcttt tagggcatcc tttcatttac
      961 actttttacg aaatcatggg atcactaaca aaatatcgct tgtcagttat attgtatggc
     1021 aggaaagata tgcgactgat attacagatc cccaaagtgg agagtttatg accattaaaa
     1081 ataagatgtt gctgggtgcg cttttgctgg ttaccagtgc cgcctgggcc gcaccagcca
     1141 ccgcgggttc gaccaatacc tcgggaattt ctaagtatga gttaagcagt ttcattgctg
     1201 actttaagca tttcaaacca ggcgataccg tcccagagat gtaccgcacc gatgagtaca
     1261 acattaagca gtggcagtta cgtaacctgc ccgcgcctga tgccgggacg cactggacct
     1321 atatgggtgg cgcgtacgtg ttgatcagcg acaccgacgg taaaatcatt aaagcctacg
     1381 acggtgagat tttttatcat cgctaaaaaa agccccctca tcatgagggg gaaatgcaga
     1441 caccttgtta ttttttatta ttagccactt gctcgtcttg cttgttatta gtcgtatttc
     1501 acgttgatta atgcggttgc ctccagtgcg ccagatttaa ctttgtttgt atcgtagacg
     1561 tagtaactgg cagttatcgg aattgaaacc gttgtatctg taagttttga ctttgccgca
     1621 atttctttta aatttgtgta tcgtttactg ctgttagtga tatttaaaag tgtgactgac
     1681 gaaccattac tttcaagaat aaatc
//
LOCUS       DAZGJW010000396         1705 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZGJW010000396 DAZGJW010000000
VERSION     DAZGJW010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.400"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..324
                     /locus_tag="ACHKIK_005444"
     CDS             <1..324
                     /locus_tag="ACHKIK_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO9086368.1"
                     /translation="ASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKAKTP
                     DYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGTVVV
                     VGRQG"
     gene            667..>1705
                     /locus_tag="ACHKIK_005445"
     CDS             667..>1705
                     /locus_tag="ACHKIK_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9086369.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVQTTSQGDRNPASVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRSFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAM"
BASE COUNT          415 a          424 c          526 g          340 t
ORIGIN      
        1 gcatcaatgg cgttatgggg ttcagccctg gcagccggtg gtttttctgc caccacaatg
       61 ctgttttcac ttggagccag catgattctg ggcggtgtgg cccagatgct ggccccgaag
      121 gcaaaaacac cggattaccg cgcaacggat aacggcagac agaacacgta cttttcctcg
      181 ctggataaca tgattgccca ggggaacccg atgccggtgc cttacgggga aatgctggtt
      241 ggctcccgcc gtatatccca ggacatcagc acccgtgatg aaggcggggg cggaacggtc
      301 gtggttgtcg ggcgacaggg ataaaacata aaaaaatccc gcagtgatcg cggagctgcg
      361 gggacagaca aatgaagatc aatgttaagg agttgttttt gttactcggg caaaaaaaca
      421 ctaacgcagc gaaattataa gcgccacagt cagtgtgtga aaatgtgaag atattcagaa
      481 tttttatgcc attaccggtt ttaaccaaca ggattatcgg tgggcatgaa agaaaacccc
      541 ggtatctgct gataccgggg tttctcttta gcatggcaga aatgtgtttc atgcttttcg
      601 ggcgaaggat atccgacttc tgtacggaat ggcaagtggc ggttaattta ttcaggggaa
      661 ggctgtatgg gaaaaggtgg cggtaaggca cacacgcctc gtgaggcgaa ggataatctc
      721 aaatccacgc agatgatgag tgtgattgat gcgattggtg agggaccgat agaaggtccg
      781 gtgaagggac tgcagagtat tctggtgaac aaaaccccac tgacggacac ggacggcaat
      841 cccgtgatac acggtgtgac cgcggtctgg cgtgccgggg agcaggagca gacaccaccg
      901 gaaggctttg agtcctccgg agctgaaacc ggactgggcg tggaagtgac gaaggcaaag
      961 ccggtgacgc gcaccattac gtccgcgaac attgaccgcc tgcgggtcac cttcggggtg
     1021 cagtcactgg tgcagaccac ctcacagggt gaccgtaacc cggcatccgt ccgcctgctg
     1081 attcagctgc agcgtaacgg taactgggtg acggaaaagg acgtcaccat taacggcaag
     1141 accacctcac agttcctggc ctcggtgatt ctggataatc tgcctccccg gtcctttaac
     1201 atccggatgg tcagggagac ggcggacagc accacggacc agctgcagaa taagacgctg
     1261 tggtcgtcat acaccgaaat catcgatgtg aaacagtgct acccgaacac ggccattgtg
     1321 gggctgcagg tggatgcgga gcagttcggc ggccagcaga tgacggtgaa ctaccatatc
     1381 cgcggtcgca tcatccaggt gccgtcaaac tatgacccgg aaaaacgcac gtacagtggt
     1441 atctgggacg gcagtctgaa accggcatac agcaacaacc cggcctggtg tctgtgggac
     1501 atgctgactc acccgcgcta cggcatggga aaacgtctgg gggcggcgga tgtggacaag
     1561 tgggcgctgt atgccatcgg gcagtactgc gaccagacgg tcccggatgg tttcgggggg
     1621 accgagccgc ggatgacctt taatgcgtac ctggcacaac agcgtaaggc gtgggacgtt
     1681 ctcagtgatt tctgctctgc gatgc
//
LOCUS       DAZGJW010000397         1701 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZGJW010000397 DAZGJW010000000
VERSION     DAZGJW010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.401"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..67)
                     /locus_tag="ACHKIK_005446"
     CDS             complement(<1..67)
                     /locus_tag="ACHKIK_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9086370.1"
                     /translation="MKHTDIRAAVLDALEQHEHGAT"
     gene            complement(64..639)
                     /locus_tag="ACHKIK_005447"
     CDS             complement(64..639)
                     /locus_tag="ACHKIK_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9086371.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPSASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPAIKLGIRMLGHRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPEARYAKGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVNRIRRERLPKELGYALKQQLRIVIKR"
     gene            complement(654..1007)
                     /locus_tag="ACHKIK_005448"
     CDS             complement(654..1007)
                     /locus_tag="ACHKIK_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO9086372.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGISAQFTSGAQRGGEVQGVFD
                     DPESLGFASSGIRIEGSSPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(1000..1374)
                     /gene="gpFI"
                     /locus_tag="ACHKIK_005449"
     CDS             complement(1000..1374)
                     /gene="gpFI"
                     /locus_tag="ACHKIK_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO9086373.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEMQS
                     SGDDVQVMNMDIRERENAAHDVREETSGALTRIRVLTCLHLCGVDGETGESVELADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            complement(1426..>1701)
                     /locus_tag="ACHKIK_005450"
     CDS             complement(1426..>1701)
                     /locus_tag="ACHKIK_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO9086374.1"
                     /translation="VWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGAVVSFKGYYGD
                     LAIVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK"
BASE COUNT          389 a          530 c          375 g          407 t
ORIGIN      
        1 gcgtcgcccc gtgttcatgc tgctcgagtg catccagcac tgcagcacgg atatcagtat
       61 gtttcatcgc tttatcacaa tccttagttg ttgtttcaac gcatagccca gttcttttgg
      121 cagacgttct cgccggatac ggttaacgtt ctcatcaaaa gcctgcttca gtggggccgc
      181 catcggaatt ttaaccacct gaatgggaag acgattacgc tttttccttc ctttatcgtc
      241 attgcccttc gcataccggg cttctggcaa acgttgcata acatgccagc gcccattatt
      301 taatcgctgg atgaatgccc gctgataacg atgctgaccg gctttaagta tgctgttcgg
      361 acgatgacca agcatcctga tccccagctt aatagcaggg agatcaccgc ggttaacgat
      421 aattctggca ttcggatttc tgaccgtcgc ccgtttcagt ctggaccgtt cctttaccag
      481 tttccggctc accctggtct cccgggcaac ctgtgatgaa gactgattaa tcgccgttgt
      541 agccacgcgg ttaatagcca ttgctgaagc cgacggaatg gcgtttttac gaacccggct
      601 cagattttca atcgcctgat caagcccttt tatcgccata attcaccctg cgtttatcgt
      661 cgccggttaa ctgcgggtgg ttgcccacgg ttcagccaga gataacaact gcccccgtca
      721 tccggagaaa cacgatccac ccagaacgtc tcaccattaa tggtcaacgt gtcaccacgc
      781 cgcacggcac gaaccgtatc cgtccgcaca aataatgacg ggctgcttcc ttcaatacgg
      841 atcccactac tggcaaaccc cagcgactcc ggatcgtcaa aaaccccctg aacttcgccg
      901 ccacgctgtg caccggaggt gaactgcgca ctgatgccca tcacttcaac aatcgtactg
      961 tccaccccgg caagggcggc atcaaaggca ttctgaaaat cacgcataaa cagccattcc
     1021 accatcaacg tgtgtttttg catctgagga cataatcaga atcacccgac caacatccgc
     1081 aagctcaacg gattcccccg tttcaccatc aacgccacag agatggaggc aggtcagaac
     1141 tctgatgcgc gttaacgcgc cggatgtttc ctcacgaaca tcatgagccg cgttttccct
     1201 ctcccggata tccatattca taacctgtac atcatcgccg gatgactgca tttcctcttc
     1261 ccattctgcc acccgctgcg ctatctctgc ggcactcccg gatatatccg gctcacgccc
     1321 cagaatcagg gccagttcat caagccgttt cagattttgc tctttcgttg ccatatcatc
     1381 cccctgtgaa aaaagacacg ggggcatttc gcccccgctc acggattatt tcacctgtac
     1441 caccacaaac tcatccgggt ccggcaacac catcagcggc gcggactgcg tcatggtaaa
     1501 ttcacgggcg ggatccccta ccgtcagcca gtgtttcgga taacgggaag aggccaccac
     1561 aatggccaga tcgccgtaat accctttgaa ggacaccacc gcgcccaggt ctttcactgc
     1621 cgtttcgagt tgtgaatttg agccgcgacg ggtatccagt ttttcgcgga acaatttaaa
     1681 gccattcagc agacgccaga c
//
LOCUS       DAZGJW010000398         1690 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZGJW010000398 DAZGJW010000000
VERSION     DAZGJW010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.402"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1371
                     /locus_tag="ACHKIK_005451"
     CDS             <1..1371
                     /locus_tag="ACHKIK_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO9086375.1"
                     /translation="RNISTRNSFSAETYRDEFNRFIKRFPVIGSSTHSIINSIGKGAL
                     LDYVIIDEASQQDIVPGILGLGCARNVIVVGDRKQLPHVPVLLPNSPSPPAEYYNCEK
                     YSLLDSVCMLFRNMVPVTLLKEHYRCHPKIIQFCNKQFYDNALIPLTLDSGEASLSLI
                     ITAKGNHTRNFSNLRELESLEGHYWDEESSRGYIAPYNAQVNLAETVLPADFVKSTVH
                     KFQGRECDEIVFSTVLDKKRSSQHSRNIAFVDNPELVNVAVSRARNKFTLVTGNDVFE
                     RHAGHIAALIRYIKYYADDGEIFESPVISAFDLLYSEYDKSLERLNSRLNSNDSHFKS
                     EQIVACLLRDILSQDSYRSMMFHSQIALNQLVLLERGDFTHREQLFMRNRASCDFVVY
                     YKVGKTPLGVIEVDGGYHLTSVQAERDELKNSILKKCGLPLLRLRTIDSDIEGKLGAF
                     LSGLTG"
BASE COUNT          482 a          307 c          397 g          504 t
ORIGIN      
        1 agaaatattt caacaagaaa ttcattctcg gcagagacat acagggatga atttaaccgt
       61 tttataaagc gttttccggt tattggtagc agcacgcatt ctattattaa ctctatcggt
      121 aagggggctc ttcttgatta tgtgattatt gatgaagcct cacagcagga tattgttccg
      181 ggcattctgg gactgggatg tgcccgtaat gtaatcgttg taggggatcg aaagcaactt
      241 ccacatgttc ctgtattact cccgaacagc ccatctccac cggcagaata ctataattgt
      301 gagaaatata gtctacttga ttcggtttgt atgttgttca ggaatatggt gccagtaact
      361 cttcttaaag agcattatcg ctgtcatcct aaaatcattc aattttgtaa taagcagttt
      421 tatgataatg ctctgatacc gctaacgctt gattcaggtg aagcctcatt atctctgatc
      481 atcacggcaa aaggaaacca tacccgtaat ttttcaaatc tcagggagct tgaatctctg
      541 gaaggacatt actgggatga ggaaagtagc aggggatata tcgccccata taatgcacag
      601 gttaatctgg ctgaaacagt gctgcctgct gattttgtga aatcgacggt gcataaattt
      661 cagggacggg agtgcgatga aattgtgttt tccactgtgc tggataagaa gcgcagcagc
      721 cagcacagtc ggaacatcgc gtttgttgat aatcctgaac tggtgaatgt cgctgtttca
      781 agggcccgaa ataaatttac gctggtgact ggtaatgatg tatttgaacg ccacgctgga
      841 catattgcgg cgctgattcg ttacatcaaa tactacgctg atgacggaga gattttcgag
      901 agcccggtca tatcagcatt tgatttgctg tacagtgagt atgacaaatc actggaacga
      961 ctgaatagta gattgaattc aaatgattca cactttaagt cagaacagat tgtggcctgc
     1021 ctgttgcggg atatcctgtc gcaggatagt tatcggagta tgatgtttca ttcacaaata
     1081 gcgctgaatc agttggtgtt gctggagcgg ggggatttta cacaccgtga acagttattc
     1141 atgcgtaacc gggccagttg tgactttgtt gtttactata aggtcgggaa gactccatta
     1201 ggggttattg aagtggatgg aggttatcat ctgaccagtg ttcaggctga acgtgatgaa
     1261 cttaaaaaca gtatactgaa gaaatgcggc ctgccattgc taagattacg tactatagac
     1321 agtgatatag aagggaagtt gggagcgttt ctgtcaggcc tgacaggtta aagtctgaat
     1381 aaaaaatatt gcagcgggag gtctcaaacc tgatttggtc atgtgccaca cacaattgta
     1441 cgaaccccat ggacacaggc ttctttgttt tggttacatc aacgtacgta ttcctgatac
     1501 atataaaaat aagtgctatt gctcaaagct aacagggaat ggttctgttg aataattcct
     1561 tagtgaataa actttatcag ggggtaattc tattacagtg aatacttcct gcaaaatgat
     1621 atttgaattg ttcaccatct gcatcatatt tccttattta caagttgaaa ggatggtata
     1681 tgttgaaaat
//
LOCUS       DAZGJW010000399         1688 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZGJW010000399 DAZGJW010000000
VERSION     DAZGJW010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.403"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(98..1678)
                     /locus_tag="ACHKIK_005452"
     CDS             complement(98..1678)
                     /locus_tag="ACHKIK_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO9086376.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMGK
                     FIPVLAIPLIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKEKDDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVAADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLFIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRRAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGLANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPLAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEETAIANIPVHDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEAAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIC"
BASE COUNT          538 a          414 c          312 g          424 t
ORIGIN      
        1 aatggtaata catcttttac accaaatctg tcttcacaaa agtcccattg tgtgcaatat
       61 gttcctttac gagaaattga ggtattaaaa atatccatca acatatcccc atgtaattat
      121 ccatatcccg acgtagggcg atgacttttg taccgataat gacttgtaag gtattttgat
      181 caagtttcac aaccgcaatt cctccggccc tttttattgc agcctcatct atcaatccca
      241 tatccgcgat agttaaacgc aaacgagtta cacaattatc cagtgattta atattttctt
      301 taccacccag agccttaacc atttgctccg ctgcctgcaa ctctgattta tcatgaactg
      361 ggatgttagc tattgcagtt tcttcgtgag cgttcagcgt gacaggactg gactcttcac
      421 ggcctacagt catcaggtta aatttgagaa tggtccaacg gaacacaaag taataaatga
      481 agaaataagc cacttcaaca cataccgcta atggccatcc cgttcttgtg ccacgtaaga
      541 caccaaagct aataaattga atcagatcgc cagtaaagcc catcttcacg cccatatatg
      601 gcagaaccat gttagccaat ccattcataa aggcatggaa gacaaacaac agcgggctga
      661 caaataagaa cagaaattca atcggctcgc tgacgccgcc aataattacc gtgagcacac
      721 cggaaatcag caatgctttg atagcccttc tgttttgagc atgagcagtg cgatacatcg
      781 ccagcgcggc tccagccaga ccatactgaa tcatcagttt gccctgctcc attttccccg
      841 ccaaatccag cggaatgacc gcattctctt tgacgtatgc cataaacatg ttcagggtgc
      901 cgtaatactc ttcaccgcca atcactgccg atccgccgat tggggtaaag cggaaaactg
      961 atgtcaccaa gtgatttaag ccaaaaggta tcgtcacgcg ttcggcaaca gcatagaaga
     1021 aataaccaac aggaccggaa gttgaaatcc agtgaccaat gagcatgaaa agattgaaaa
     1081 atggcggcca gataaaagga atgaacagac caaatccact catcacaatc agtgtaatga
     1141 taggcacaag gcgtgggcca ctataaaacc ctaatgattc aggaatacgt aagttaacga
     1201 tctttttata catgctggcg actaataacc ctgccacaat cccacccaac acgctggtat
     1261 tgtaggactg gatcccgagt atgatggttt gcccatgtgt cgacatttgg tcagcagcta
     1321 ccaataagtc gtgctgttta agataaaagt tcgttccaag atgcatcgcc ataaagccaa
     1381 ttaagccaga aaaagcgcca taggctttat catctttctc ttttaataat cccaaaggta
     1441 tagctatcgc aaacaataca ggtaaattaa cgaaggcaaa caaaccaagg ctaacaataa
     1501 aatcaagtat ggttttaatt aacggaatag ccaacactgg aatgaatttt cccatatcat
     1561 cgctggctaa accacttccc agccctaaca tcataccaca tacacttaac agagcgatgg
     1621 gatacataaa tgccttcccc aggctctgaa aaaaactcca ggctttcttt tgtttcatac
     1681 tggttatc
//
LOCUS       DAZGJW010000400         1673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZGJW010000400 DAZGJW010000000
VERSION     DAZGJW010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.404"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..453)
                     /locus_tag="ACHKIK_005453"
     CDS             complement(<1..453)
                     /locus_tag="ACHKIK_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO9086377.1"
                     /translation="MPGLVSYISSTSFANEMAEMRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGLGLVYAELLNIGFNDGVDALRNRVLSVLPGMVAQRQENSLQAKISE
                     CTFTVDIEKLHCPGEVLQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAF"
     gene            complement(744..1319)
                     /locus_tag="ACHKIK_005454"
                     /pseudo
     CDS             complement(744..1319)
                     /locus_tag="ACHKIK_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
     gene            complement(1432..1671)
                     /locus_tag="ACHKIK_005455"
     CDS             complement(1432..1671)
                     /locus_tag="ACHKIK_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO9086378.1"
                     /translation="MCGCGKHDGREHVQSLTAQLRLGPADILESDENGIIPEQDRVIT
                     QVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          472 a          461 c          283 g          457 t
ORIGIN      
        1 aaatgcagcg gcatcaaata aagtacatac atctgaacca tctgaattct tcacaaaaat
       61 acctttttca ggctgctcca gtgtaattgg acattgaagc acctcaccag gacagtgaag
      121 tttttcaata tcaacagtaa aggtacattc cgatattttg gcctgcaaag agttctcttg
      181 tcgctgcgcc accattccag gaagcacgct taacaccctg tttctgagcg catcaactcc
      241 atcattaaaa ccaatattca ataattctgc ataaaccagt cccagccctt cactttctgc
      301 taaaaaacgg cctgtatctg gcatataaga aactctaact ctctcccctc ccaggagaaa
      361 tcctccaatc tgcccttcca ttacctgctg acgcatttcc gccatctcat tcgcgaatga
      421 agtcgatgat atatatgata ctaatccagg cattaccata ttccttcata cagataaata
      481 tttcctctgg gcttcataac aaacatctcc ctgacatgac aacaaaaacc ggagccggac
      541 tccggttttt gtgaatccgt cggctatttc atcccgccaa tatttccccg tcagcacgcc
      601 agatttgcag cggcctcacc actcactgta tatgcttttt agctgcatcc agtacaccaa
      661 ttaccacatc cttataccct gaacatctgt agcatttcat ataagcaact ggcagttaac
      721 atatagcttg ggtaatattt atttcactta tcctttatga caaagtttcc tttggtttgg
      781 tcataaatac attgttcttg acttacaatc attgatgacg ttattggttc tcgtgttagc
      841 gggtgcttcc caccgtcgcg aaccaaatga gaaaaagaaa ccgaatcaaa taaggtacaa
      901 accaaagaac cttctgaatt tttaacaaac acaccttctt ctggtttatc cagtataatt
      961 gggcatcgaa ccgcctcgga tggacactga aggctctccg tattaacaga aaacttacat
     1021 tgtgatattt tcccctcaat atcaccatgt gattgtgtgt tacctgaacc tgaaaccatt
     1081 cttaacatta tatccctgag cgcttcagga ccaccattaa gccctgtaag taataatcca
     1141 gaaagcaaac tgttattgcc cccactcgcc agaaagcgac catccgtttc actatatgtg
     1201 atacggaccg tctacccgcc tactgtaatt tctccaccgc cactggcggc catttgagca
     1261 cgaagaacct gcattccggt ggaaaatgaa ttggatgcaa tagctgatgt taacggcatg
     1321 acaaaacacc ctatttaaat attcattcca aacacaggga gtcataacaa acagccccca
     1381 tacatgacaa caaaaaccgg agccggactc cggttttgtg aagctgtcgg gttacttcat
     1441 cccgccaata ttttcccacg tcccgtcagc acgcagaatt tgcagcggtc ttaccacaca
     1501 ctgtatctgc tttttatccg catccagtat caccacctgc gtgattaccc tgtcctgctc
     1561 cgggataata ccattctcat cggactccag gatgtctgcc ggccccagtc gcagttgtgc
     1621 tgtaagcgac tgcacgtgtt cacggccatc atgctttccg caaccacaca gac
//
LOCUS       DAZGJW010000401         1665 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZGJW010000401 DAZGJW010000000
VERSION     DAZGJW010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.405"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            49..1665
                     /gene="tir"
                     /locus_tag="ACHKIK_005456"
     CDS             49..1665
                     /gene="tir"
                     /locus_tag="ACHKIK_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFO9086379.1"
                     /translation="MPIGNLGHNPNVRALIPPAPPLPSQTDGAGGARNQLINSNGPMG
                     SRLLFTPIRNSVADAADSRASDIPGLPTNPLRFAASEVSLHGALEVLHDKGGLDTLNS
                     AIGSSLFRVETRDDGSHVAIGQKNGLETTVVLSEQEFSSLQSLDPEGKNKFVFTGGRG
                     GAGHAMVTVASDIAEARQRIIDKLEPKDTKETKEPGDPNSGEGKIIEIHTSTSTSSLR
                     ADPKLWLSLGTIAAGLIGMAATGIAQAVALTPEPDDPITTDPDAAANTAEAAAKDQLT
                     KEAFQNPDNQKVNIDENGNAIPSGELKDDVVAQIAEQAKAAGEQARQEAIESNSQAQQ
                     KYDEQHAKREQEMSLSSGVGYGISGALILGGGIGAGVTAALHRKNQPAEQTITTRTVV
                     DNQPTNNASAQGNTDTSGPEESPASRRNSNASLASNGSDTSSTGTVENPYADVGMPRN
                     DSLARISEEPIYDEVAADPNYSVIQHFSGNSPVTGRLVGTPGQGIQSTYALLASSGGL
                     RLGMGGLTGGGESAVSTANAAPTPGPARFV"
BASE COUNT          465 a          373 c          434 g          393 t
ORIGIN      
        1 atataaagga ttattatcta tttacataaa ataaaaagga gatattttat gcctattggt
       61 aatcttggcc acaatcccaa tgtgagagct ttaattccac ctgcaccgcc attaccttca
      121 caaaccgacg gtgcaggagg tgcccgtaat cagctcatta actcaaatgg cccgatgggg
      181 tctcgtttgc tatttacgcc tataaggaat tctgttgctg atgctgctga ttctcgtgcc
      241 agtgatattc ccggacttcc tacaaatcca ctgcgctttg ctgcgtccga ggtatctttg
      301 catggtgcgc ttgaagttct tcatgataaa ggggggcttg atactcttaa ctctgctatt
      361 ggatcttcgt tattccgtgt tgaaactcgg gatgatggca gccatgttgc tatcgggcaa
      421 aaaaatggcc tcgagaccac tgttgtttta agtgagcaag agttttctag cttacagtcc
      481 cttgatcctg aaggtaaaaa caaatttgta tttactggag gccgcggtgg cgcagggcat
      541 gctatggtca cggttgcttc agatatcgcc gaagcccgtc agaggataat agataaatta
      601 gaaccaaagg atacaaagga gacgaaggag ccaggggatc caaatagtgg cgagggaaaa
      661 atcattgaaa ttcatacctc aacctcaact tctagcctcc gtgcagatcc taaactttgg
      721 ttgtcattgg ggactattgc tgcaggtctg atagggatgg ctgcgacggg gattgcacag
      781 gctgttgcgt tgactccaga gccggatgac ccaatcacta ccgaccctga tgctgcagca
      841 aacacagctg aagcagcggc aaaagatcag ttaacgaaag aagcattcca gaacccagat
      901 aaccagaaag ttaatatcga tgagaacgga aatgcaattc cgtccgggga actaaaagat
      961 gatgttgttg cgcaaatagc agaacaagct aaagcggcgg gtgaacaggc cagacaggaa
     1021 gctattgaaa gtaattctca ggcgcagcaa aaatatgatg aacagcatgc taaacgcgaa
     1081 caggaaatgt ctctttcatc gggggttggc tacggtatta gtggtgcgct gattcttggc
     1141 gggggaattg gtgccggtgt tactgctgct cttcatcgga aaaaccaacc ggcagaacaa
     1201 acaatcacta cacgtacggt agtcgataat cagcctacga ataacgcatc tgcgcagggc
     1261 aatactgaca caagtgggcc agaagagtcc ccggcgagca gacgtaattc gaatgccagc
     1321 ctcgcatcga acgggtctga cacctccagc acgggcacgg tagagaatcc gtatgctgac
     1381 gttggaatgc ccagaaatga ttcactggct cgcatttcag aggaacctat ttatgatgag
     1441 gtcgctgcag atcctaatta tagcgtcatt caacattttt cagggaacag cccagttacc
     1501 ggaaggttag tgggaacccc agggcaaggt atccaaagta cttatgcgct tctggcaagc
     1561 agcggcggat tgcgtttagg tatgggagga ttaacggggg ggggcgagag cgcagtaagt
     1621 actgccaatg ccgcaccaac gccgggaccc gcacgtttcg tttaa
//
LOCUS       DAZGJW010000402         1663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZGJW010000402 DAZGJW010000000
VERSION     DAZGJW010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.406"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..588
                     /locus_tag="ACHKIK_005457"
     CDS             <1..588
                     /locus_tag="ACHKIK_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFO9086380.1"
                     /translation="IFNKKIMPHHKIQLHCITKDGELSPPVLIRKEDAVERMDYSYHY
                     NKNEGKGFSTIGMLKNISIFRGRFNSKEITEHVFHTTKFSGDEKYIKFHCNSVEELKP
                     SKLDVIAKPGDILIARVGRNFHKKILFVESGYSYISDCIFLIRASGGDKKKLFDFLCS
                     QDGQEELSRASSGVAAQHITMDALKKIHLVRIKHD"
     gene            581..>1663
                     /locus_tag="ACHKIK_005458"
     CDS             581..>1663
                     /locus_tag="ACHKIK_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO9086381.1"
                     /translation="MTDVELDSNFLIEERADYIDEDTIKNNTIRSGEFFDIIHNALIS
                     PGIKLIVGPRGCGKTHLMRYAWVECKNDDNAPLPLYVSFNKYFKLEPLLKNKPNAIDL
                     FHKWVLAKILLSAYEIACDIEDSEYLDLSTILIADKEFLINLIVRLEQGLSPSLEQEN
                     IVQLISVSSVISSLSSLCDWLERKRVIILLDDAAISLTPEYLYEFFDIVRSLKTSKIA
                     LKASVYPGTTEYGPRFHVAHDAETIDAWISVQHPNYSSVMDAIAQARFPGYDGIPDDL
                     KELFKFSAFGIPRSFLMMLRDYQTTLSQTTQQKFNKIIERYVELRLSEYSSLKDKLPR
                     LFDIVSLGESLLLKVVELLKESNSQYK"
BASE COUNT          562 a          244 c          314 g          543 t
ORIGIN      
        1 atttttaata aaaaaattat gcctcatcat aaaatacaat tacattgtat aactaaagat
       61 ggggaattgt cgcctcctgt tttaattaga aaagaagatg cggttgagag aatggactac
      121 tcttatcatt ataataaaaa tgaaggtaaa gggtttagca caatagggat gcttaaaaat
      181 atttcaattt ttagaggtag gtttaattca aaggaaatta cggaacatgt ttttcatacg
      241 acaaaattta gtggtgatga aaagtacatt aaattccact gcaactctgt agaagaattg
      301 aagccatcaa aattagatgt cattgctaag cctggtgata tattaatagc aagagttggg
      361 cgaaattttc ataaaaaaat attgtttgtt gagagcggct attcttatat cagtgactgt
      421 atttttctga tacgagcctc tggtggagat aagaaaaaac tatttgattt tctttgttct
      481 caagatgggc aagaggaatt atctcgagcg agtagtggtg tagccgcaca acatattaca
      541 atggatgcat taaaaaaaat acatcttgta aggattaaac atgactgatg tggaattaga
      601 tagtaatttt cttatcgagg aacgagctga ttatattgat gaggatacga taaaaaataa
      661 tactatcaga tctggagagt ttttcgatat tatacataat gctcttatat ccccaggcat
      721 caaacttata gttggtccta ggggatgtgg taaaacacat ttgatgcgtt atgcttgggt
      781 tgaatgtaaa aacgatgata acgcaccatt accactctat gtttctttta ataaatattt
      841 taagttagag ccgcttttaa aaaataaacc aaatgcaatt gatctctttc ataaatgggt
      901 attagcaaag atactactct ctgcttatga aattgcttgc gatattgaag attctgaata
      961 tcttgatttg tcaacgatac tcatagcgga taaagagttc ctaattaatt taatcgtaag
     1021 gcttgagcag gggctttcgc catcattaga acaagaaaac attgttcaat tgatcagtgt
     1081 ttcttctgtc atatcctcgt tgagttcact atgtgattgg cttgaacgta aaagagtcat
     1141 tattttatta gacgatgcag caataagctt aactcctgaa tatctttacg agttttttga
     1201 tattgtacgt agtctaaaaa cctcaaaaat agctttaaag gcatcggttt atcctggaac
     1261 aaccgaatat ggcccaaggt ttcatgttgc tcatgatgct gaaacaattg atgcttggat
     1321 ttcggtacag caccctaatt attcctcagt gatggatgct atagcgcagg caaggtttcc
     1381 tgggtatgat ggcattccgg atgacttgaa agagttattt aaattctcag catttggtat
     1441 tccacgttct tttttgatga tgttaagaga ttatcaaaca actttaagtc agacaactca
     1501 gcaaaaattt aataaaataa ttgaaaggta tgttgagctt agactcagcg aatacagttc
     1561 actaaaagat aagctacctc gattgtttga cattgtttca ttaggtgaat cacttttatt
     1621 aaaagtagtc gaattactca aggaaagtaa ctcccaatat aaa
//
LOCUS       DAZGJW010000403         1655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZGJW010000403 DAZGJW010000000
VERSION     DAZGJW010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.407"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..874
                     /locus_tag="ACHKIK_005459"
     CDS             <1..874
                     /locus_tag="ACHKIK_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system protein SepQ"
                     /protein_id="HFO9086382.1"
                     /translation="YLPLLSVIGTGRLYITPEGHACHAYFREVSGNGIRFTLTCSGYE
                     GRFWISEEQFIQWCQELFPYSESRLIPEDMIKLMILWVMQTALPEGDVSVDDVQFTML
                     NKDVYPVIENNNGENRLNVIILEITVQSLQYLINENWQFVPHSNTIFFDGYIAPGWTD
                     YPVTELTVGDSLRLYHVDDSKERECWLVINNPLATVKLCDNNLFIADVQAADLLCTIS
                     NEAVMDRIYCVIGTIHVDIHMLRNAKKDDIIDSTGYHLFGGCRLIRNNTTIAYGSIVK
                     INEDFYFTVSLVCD"
     gene            905..1420
                     /locus_tag="ACHKIK_005460"
     CDS             905..1420
                     /locus_tag="ACHKIK_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspH"
                     /protein_id="HFO9086383.1"
                     /translation="MSSSLSGIILNTSLTSHHMSWGKFSRTLHVLNEDIVDEIQLKAR
                     TQQRHHRVYSEMADYSVVSFRGKEYSVRFIKDYKTDNYVYKVQKMTQESGCLSRLFSF
                     LSGGVTKALESKLNERHITPLSPTWFPRTPLEGVLTERGLSSLLRRVQSTENLDYSNA
                     MISNSFSNSVV"
BASE COUNT          497 a          269 c          353 g          536 t
ORIGIN      
        1 ttacttgcca ttgttgtcag tcataggaac cggtcgcctc tatattacgc ctgaagggca
       61 tgcttgccat gcatattttc gcgaagtcag cggtaatggt atccgcttca cgttaacatg
      121 ctcaggctat gaggggcgtt tttggatatc agaagaacag ttcattcagt ggtgccagga
      181 gttattcccg tatagcgaaa gccggctgat accggaagat atgataaaac tcatgatctt
      241 atgggtaatg cagactgctt taccagaagg tgatgtgtct gttgatgatg ttcaatttac
      301 gatgctaaat aaagatgttt atccggtaat agaaaataat aatggagaaa acagacttaa
      361 tgtaattata ctcgaaataa cggtacagag tttacaatat ctaattaatg aaaattggca
      421 atttgttcca cattctaata ccattttctt tgatggatat attgctcctg ggtggactga
      481 ttacccggtt acagagctga ctgttggtga tagtttaagg ctctatcatg ttgatgacag
      541 caaagaaaga gagtgttggc ttgttattaa taacccttta gcaaccgtaa aactttgtga
      601 taataattta tttattgcgg atgtccaggc tgcagatttg ttatgtacta tcagcaatga
      661 ggctgtaatg gatcgcattt attgtgttat tggaacaatt catgttgata tacatatgtt
      721 gcgcaatgcc aaaaaagatg atattatcga ttcaactgga tatcatcttt ttggtgggtg
      781 ccggttaata cgcaataata cgactatagc atacggaagc attgtaaaaa tcaacgaaga
      841 tttttatttt actgttagtt tagtatgtga ttaatcagga ataagttata tagggagatg
      901 tattatgtcg tcatcgttat caggaataat actcaatact tcgctgacaa gtcaccatat
      961 gtcatggggc aagtttagca gaactcttca tgtattgaat gaagatatcg tagatgaaat
     1021 tcagcttaaa gcccgaacac aacaacgcca ccatcgagta tattcagaaa tggcggatta
     1081 ctccgttgtc tcctttcggg gcaaagaata ttctgttcgt tttattaaag attacaaaac
     1141 agataattat gtgtacaaag tacaaaagat gactcaggaa agtgggtgtc tctcaagatt
     1201 atttagcttt ctttctggcg gtgtgactaa ggctttggaa agtaaactaa atgagaggca
     1261 tatcactcca ctttctccaa cctggtttcc tcgaactcca ctggaggggg tcttaacgga
     1321 aagaggatta tcttctttgt tacgtcgcgt acagtctact gaaaaccttg attatagtaa
     1381 tgctatgata tcaaactctt tctctaactc cgtagtgtga ttatcatttg agctttcctc
     1441 ctggtttaac tccagaataa ttgtgattct ggagtttttc tgtttgtttt atgtcgataa
     1501 tacattgttt ttaggaaaat atgggatgaa tattcagagt gagtttggct ggcagaagtc
     1561 agagattctg tgatgaggag gggattgagc tgaatggatg aattccttcc agctcataat
     1621 ttatcaggat aaaacatctt acgacaataa actaa
//
LOCUS       DAZGJW010000404         1649 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZGJW010000404 DAZGJW010000000
VERSION     DAZGJW010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.408"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..677)
                     /locus_tag="ACHKIK_005461"
     CDS             complement(<1..677)
                     /locus_tag="ACHKIK_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO9086384.1"
                     /translation="MERLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRRVKFS"
     gene            685..1110
                     /gene="tnpA"
                     /locus_tag="ACHKIK_005462"
                     /pseudo
     CDS             685..1110
                     /gene="tnpA"
                     /locus_tag="ACHKIK_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1174..1467)
                     /locus_tag="ACHKIK_005463"
                     /pseudo
     CDS             complement(1174..1467)
                     /locus_tag="ACHKIK_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
BASE COUNT          434 a          393 c          361 g          461 t
ORIGIN      
        1 ttgctgaatt tgaccctgcg gcttaactgt cgctgaagtc tcgccagcgt cttctggttt
       61 ttctgaaaac tgtttacagg ctcaaagact gtgccatctg acagcgtggc gagtttagcc
      121 acgccagcat ccagcccgac cattgatgct gaagggtgaa ccggagtgga tacttcactt
      181 tctgtctgaa tactgatgta ccacttaccg caggactggc tgacagtgac atttttcaca
      241 actcccgtga cctgacggct attccggtag cgcatccagc caagtttcgg cagaaaaata
      301 cggctgtttt cctgatcgag cttaacgccc tgcgggtagc ggaatgcatc attctgtccc
      361 cgctttttga atcggggaaa agcagcccga ttctggaaga agtttttgta agcccgctca
      421 aggtctttca gtgactgttg caatggctgt gagggcgaat ctttaagcca ttgcgtttca
      481 gtggcgtttt tccactcaac cagccaggaa gccattttcc cgtaagggat gtatttatta
      541 ccggcctcat gattctcatt ctgacgtgcc agagcacgat tgaaaacgaa acgacatgcg
      601 cctgcgaagc gcctcatttc acgctcctgt tgaccaccgg gtcttaactg gaatttaaat
      661 gcctgtagtc gttccacagg tcaaattata ttttggtctg tgaaaaaaga aaccgatatc
      721 cggcgtggaa ggcattgtgt tttcctgatg catgttcacc tggtctttgt caccaaatac
      781 cgacgccaga tttttgacca tgacgcgaca gaaaaactac gcacttactt ttcaaatgta
      841 tgtgctgatt ttgaagctga actggttgaa atggatggcg aaccagatca cgtccatttg
      901 ttaattagct atcctcccaa actggcgata tccagtctgg taaacagcct caaaggcgta
      961 tcgggtaggt tactgcgacg agatcgacca gatattgcag tcaggtatta ctacaaaggc
     1021 gttttgtgga gtcctggcta ttttgccaat agctgcggag gtgcgccaat atccgtcatc
     1081 cgccaataca ttgaacaaca gcaaacacct ggtcaggtgg aaaaccgcgc cttatatccc
     1141 cgccctgaag gacggggttt tacggcgcac cagataaacc aggccaagcg tatgctggtt
     1201 gatcaaataa tatcccccaa gcgcagcaat attaatattc acctgcaatg ggtcgatacc
     1261 tggcttaaaa atattgtatt tcttcccttc atccaataag gattccatta actgcaagtg
     1321 cgcatggtta atttctaaga gacgctgtga ttttgcataa tgcacacctt tactttgatt
     1381 ttcactatgc acaattttta aaaaccaagg gttagcaata taataacccc atgtaaaatc
     1441 aataattttc tcaatggctt tttgtggctg gttaaccaga tgattaacaa acgcaccgat
     1501 accaacagcg tagaataatt acataccgca caacaagaat gtatggtgga aggcgtggaa
     1561 gtcattttct ccccgggggc atcgttatat ccataatgag cgatttttga tattaatatc
     1621 tttcaccatc cgctattttc actacttca
//
LOCUS       DAZGJW010000405         1643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZGJW010000405 DAZGJW010000000
VERSION     DAZGJW010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.409"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..195
                     /locus_tag="ACHKIK_005464"
     CDS             1..195
                     /locus_tag="ACHKIK_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086385.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            203..1615
                     /gene="pykF"
                     /locus_tag="ACHKIK_005465"
     CDS             203..1615
                     /gene="pykF"
                     /locus_tag="ACHKIK_005465"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO9086386.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          447 a          411 c          397 g          388 t
ORIGIN      
        1 ttggactgta gaactcaacg actcaaaaac aggcactcac gttgggctga gacacaagca
       61 cacattcctc tgcacgcttt ttcgatgtca cctatcctta gagcgaggca ccaccacttt
      121 cgtaataccg gattcgcttt ccggcagtgc gcccagaaag caagtttctc ccatccttct
      181 caacttaaag actaagactg tcatgaaaaa gaccaaaatt gtttgcacca tcggaccgaa
      241 aaccgaatct gaagagatgt tagctaaaat gctggacgct ggcatgaacg ttatgcgtct
      301 gaacttctct catggtgact atgcagaaca cggtcagcgc attcagaatc tgcgcaacgt
      361 gatgagcaaa actggtaaaa ccgccgctat cctgcttgat accaaaggtc cggaaatccg
      421 caccatgaaa ctggaaggcg gtaacgacgt ttctctgaaa gctggtcaga cctttacttt
      481 caccactgat aaatctgtta tcggcaacag cgaaatggtt gcggtaacgt atgaaggttt
      541 cactactgac ctgtcagttg gcaacaccgt actggttgac gatggtctga tcggtatgga
      601 agttaccgcc attgaaggta acaaagttat ctgtaaagtg ctgaacaacg gtgacctggg
      661 cgaaaacaaa ggtgtgaacc tgcctggcgt ttccattgct ctgccagcac tggctgaaaa
      721 agacaaacag gacctgatct ttggttgcga acaaggcgta gactttgttg ctgcttcctt
      781 tattcgtaag cgttctgacg ttatcgaaat ccgtgagcac ctgaaagcgc acggcggcga
      841 aaacatccac atcatctcca aaatcgaaaa ccaggaaggc ctcaacaact tcgacgaaat
      901 cctcgaagcc tctgacggca tcatggttgc gcgtggcgac ctgggtgtag aaatcccggt
      961 agaagaagtt atcttcgccc agaagatgat gatcgaaaaa tgtatccgtg cacgtaaagt
     1021 cgttatcact gcgacccaga tgctggattc catgatcaaa aacccacgcc cgactcgcgc
     1081 agaagccggt gacgttgcaa acgccatcct cgacggtact gatgcagtga tgctgtctgg
     1141 tgaatccgca aaaggtaaat acccgctgga agcggtttct atcatggcga ccatttgcga
     1201 acgtaccgac cgcgtgatga acagccgtct cgagttcaac aatgacaacc gtaaactgcg
     1261 cattaccgaa gcggtatgcc gtggtgccgt tgaaactgct gaaaaactgg atgctccgct
     1321 gatcgtggtt gctactcagg gcggtaaatc tgctcgcgca gtacgtaaat acttcccgga
     1381 tgccaccatc ctggcactga ccaccaacga aaaaacggct catcagttgg tactgagcaa
     1441 aggcgttgtg ccgcagctgg ttaaagagat cacttctact gatgatttct accgtctggg
     1501 taaagaactg gctctgcaga gcggtctggc acacaaaggt gacgttgtag ttatggtttc
     1561 tggtgcactg gtaccgagcg gcactactaa caccgcatct gttcacgtcc tgtaatattg
     1621 cttttgtgaa ttaatttgta tat
//
LOCUS       DAZGJW010000406         1614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZGJW010000406 DAZGJW010000000
VERSION     DAZGJW010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.410"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1246)
                     /locus_tag="ACHKIK_005466"
     CDS             complement(<1..1246)
                     /locus_tag="ACHKIK_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO9086387.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVERAFSSQVSVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQQLGYVVARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFSHKY
                     RLSSRKTTDLMAYIHYLSDRRIGFRNRIDLLLNFRILKVKPLVIPERRLALFTSLQLS
                     YYEKFIREKQVSLNEYEEVLKES"
     gene            complement(1487..>1614)
                     /locus_tag="ACHKIK_005467"
     CDS             complement(1487..>1614)
                     /locus_tag="ACHKIK_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO9086388.1"
                     /translation="FVAGVCAALLAVSVAIPGWQFLTQPSPEEQHFTWGNGCKKQ"
BASE COUNT          460 a          374 c          296 g          484 t
ORIGIN      
        1 cagattcctt taatacttcc tcatattcat tcagagaaac ttgtttttcc cggatgaatt
       61 tttcataata agaaagctga agagaagtaa atagtgccag ccttctctcc ggaatcacca
      121 atggtttgac tttcagtatc ctgaagttta acaacaaatc aattctgttt ctgaatccaa
      181 tccgcctgtc agaaaggtaa tgaatataag ccatcaggtc agttgttttt cgtgaggaaa
      241 gtcggtattt gtgagaaaaa agctcctccg gtcgaagatt ctgactctgc cattgctgca
      301 ggtatttata ctcaatattc agctcattaa tttctgcttt taacgaagcg acctggttaa
      361 tggcattcag gtgttttttc acttgttgaa gtacatcatc aatcgcattt gcagacggtg
      421 agtcaggtaa gacttcctca gaacgcgaaa tcgacgttga aaaaaactgt tgcctgttct
      481 ccgtactccc cagtcgtgcc accacatatc ccagttgctg tttgtccatc ttttcataaa
      541 cattactgac agctgagtta ttattggaca aaattgccac tgttttattc tgaattaata
      601 tattagcaac aatattgagt atagtctgtg ttttacctgt tccaggtggt ccttcaataa
      661 ctgaaacctg tgaggaaaat gcccgttcga cagccagcaa ctgactttca ttaagaccaa
      721 acggaaaaat caaaccccat ggcgaatcac gttttttact ctgtcctgaa cagtatgcat
      781 gcaaggcggt atccggatga ggaactattt tctttatctg cctaaggata ttctcatcga
      841 tatttctgtt attaccgcgg gcataaaaaa tacgttcatt agccacccta cacaagtaat
      901 taaacacggc atgttctgta aatgctgaac actgttgaaa gtcaagaccg gtcgttttca
      961 tgatgtaggg tcgttcaccc tcgcgataat aaacagcagc atatttatca ccatatataa
     1021 taaccagttc agcccgggat gttacagcgt tccctttcct ttcgaggaaa acatttcgca
     1081 aatttacagt tggcgttatc tgacatacag aaagcggacg agtatatttt ctcccgctgc
     1141 gaaaatgaca ggtcagcata acctctctgt cgtcatttcc agcccacagc gtccagtcgg
     1201 taattttatg agtcttattt tcaccgtcaa cacaaatcgt gaccatattc ttactcagaa
     1261 attatcattt aacttacgga aaattgttaa ggcactcccg atattagctc cggaggattt
     1321 tccacaactc cgggactgtt aacatatttc tgcctgccat atatacattg aatttagtca
     1381 taacagtcaa taccagaatt acaggaatgc gatgccgata cgctggttat tgaatactgg
     1441 tagcccctga gcactaattc atcacgatac tctgtattta ctgttttcac tgctttttac
     1501 aaccattccc ccaggtaaaa tgctgctcct ccggtgatgg ctgtgtcaga aactgccatc
     1561 ccggaatagc cacgctgacc gccagcaatg ccgcacagac accagccaca aacg
//
LOCUS       DAZGJW010000407         1593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZGJW010000407 DAZGJW010000000
VERSION     DAZGJW010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.411"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(22..90)
                     /locus_tag="ACHKIK_005468"
                     /pseudo
     CDS             complement(22..90)
                     /locus_tag="ACHKIK_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flhB HrpN YscU SpaS family protein"
     gene            complement(99..866)
                     /gene="sctT"
                     /locus_tag="ACHKIK_005469"
     CDS             complement(99..866)
                     /gene="sctT"
                     /locus_tag="ACHKIK_005469"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFO9086389.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTV
                     KSALAFLILIIYFAPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(868..1128)
                     /locus_tag="ACHKIK_005470"
     CDS             complement(868..1128)
                     /locus_tag="ACHKIK_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO9086390.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1138..>1593)
                     /locus_tag="ACHKIK_005471"
     CDS             complement(1138..>1593)
                     /locus_tag="ACHKIK_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO9086391.1"
                     /translation="IYNNSQNENLSFNNVASVVNFVETGMSGYKSYLIKYSEPELVSF
                     FEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYIYLPFVVVDLVIS
                     SVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLSINP"
BASE COUNT          546 a          328 c          267 g          452 t
ORIGIN      
        1 gatggttaag tctctacttt atagaaattg ccctttttta gaagcatcct ggagtttctt
       61 ttgtgttggc ttttcagttt tattggccat gaaacactct atttctcaat gtatagttgc
      121 aattgttctg ggaaaaagga taacggcatt actcgttcag ccagtattgg ggcaaaataa
      181 ataattaata ttaaaaatgc cagcgcactt ttgacagtca gtgaaatggc aaaagcattt
      241 agttgtgaag cataacgtgc cagcaatccc aggacagcct caccgcccag cataacggca
      301 atcacggggc ttgcgtaaac gatagtatga gtcattatgt tattaatata tgagaataaa
      361 ggctctaatt taggaaaatt aaaattgcct gaaggccata aattataact ttgccaaaga
      421 gtttccagaa taaagttcat tccaccttta gtaagataaa ccgcagcaga gaaaaggtta
      481 aataatcttg ctaattccga ggtatcgaca cctgtagccg gatccaaagt actacttaat
      541 gttgcaccgc gttggttatc gataaagcta cctatagcaa ggaaaatcca aaacggggat
      601 gctaataagc atgcaataaa cagccctatg ataatttctc tggcagcgaa caaagcaata
      661 tcgatttcat ttaagttcaa taattcatag tggtaatgct gaactaatgc acatgatacc
      721 actattatta ttgggtgacg aactactgtt ggtatattcc cactggcgaa aaatggtaac
      781 aaatagaaag ttggtgccaa tcggcaaaaa ccgagcgctg tagcagccag cagactatgc
      841 aactgataga gtatcgcctc ccccatgcta tcctctcgcg aatgctaaat tcagcatttc
      901 gataccaaat gaatataatt tttcaccata ccacccgctc attaaaaaaa agcaaatact
      961 tacgcacaat aatttcacgc caaatggcaa cgtctgttct tgcaactgcg ttaccgtttg
     1021 aaaaagccct accaataaac caacaaatgt cgcaactgca ataggtcctg cagacataac
     1081 taaaatcaga tacagcgccc tattccccgc aaaaacaata tcgtccatat ttttttctca
     1141 gggattaatg gataggtcaa aatattgtaa aatgagccct tttgataaca ttgtccaacc
     1201 gtccatcgcg acaaacaaaa ttaattttat cggtgtagat atggttaccg gactcatcat
     1261 catcatacct aacgttaaca gaacgctgga tataaccaga tccactacga caaagggtaa
     1321 atagatataa aaacctataa taaatgcact ttttatctca ctcaatgcat atgcgggcag
     1381 taacgagaaa attgaaatat tatcatcatc gattatctct tcattgtctt cagaactatt
     1441 caccttctga atcttttcaa aaaagctaac caactcaggt tcagaatatt ttatcaaata
     1501 tgatttataa ccactcatgc ctgtctcaac aaaattaaca accgatgcta cattattgaa
     1561 tgaaagattc tcattctgac tgttattata tat
//
LOCUS       DAZGJW010000408         1579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZGJW010000408 DAZGJW010000000
VERSION     DAZGJW010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.412"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1579
                     /locus_tag="ACHKIK_005472"
     CDS             <1..>1579
                     /locus_tag="ACHKIK_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO9086392.1"
                     /translation="ASAVAQNTAAAKKSASDASTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAAAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAGNSAKAAKT
                     SETNAKSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIVQLSSATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSA
                     SETLAETPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVS"
BASE COUNT          439 a          467 c          457 g          216 t
ORIGIN      
        1 acgcgtccgc ggtggcacag aacacggcag ccgcgaagaa gtcagccagc gatgccagca
       61 catcagcccg tgaggcggca acccatgcga ctgatgctgc aggctcagca cgcgcagcca
      121 gcacgtcagc cggacaggcc gcgtcgtcgg ctcagtcagc gtcttccagc gcaggaacgg
      181 catcaacaaa ggccactgaa gcatcaaaaa gtgctgccgc tgcagagtcc tcaaaaagcg
      241 cggcggccac cagtgccgct gcggcgaaaa cgtcagaaac gaatgcggca gcgtcacaac
      301 aatcagcagc cacttctgca tccaccgcga ccacgaaagc gtcagaagct gccacctcag
      361 cccgggatgc gtcggcttca aaagaagcgg caaaatcatc agaaacgaac gcatcatcaa
      421 gcgccagtag tgccgcttcc tcggcaacgg cggcaggaaa ttccgcgaag gcggcaaaaa
      481 cgtccgagac gaacgctaag tcttctgaga cggcagcggg acagagcgcc tcagctgcgg
      541 caggctcaaa aacagcggct gcgtcgtctg ccagtgccgc gtcaacaagt gccgggcagg
      601 cctcagccag tgccaccgcc gccggaaaat cggcagaaag cgccgcatca tccgcttcaa
      661 cagccacaac gaaggctggc gaagccactg aacaggccag cgcagcagcg agttctgctt
      721 ccgcagcgaa gacatccgaa acgaacgcga aagcgtcgga aaccagcgca gaatcctcaa
      781 aaacggctgc cgcatcgtca gccagttcgg cggcgtcatc ggcatcatcg gcgtctgctt
      841 caaaagatga ggcgaccaga caggcgtcag cagcgaagag cagcgccacg acggcatcca
      901 cgaaggcgac agaggcagct ggcagtgcga cggcggcagc tcagagcaaa agtacggcgg
      961 aatccgcggc aacgcgcgcc gagacagcag ctaaacgggc agaggatatt gcatccgccg
     1021 tggcgcttga ggatgcgagc acgacgaaaa aggggatagt acagctcagc agtgcgacta
     1081 acagcacttc cgagtcactg gcggcaacgc caaaagccgt taaggccgcg tatgacctgg
     1141 ctaacgggaa atacaccgca caggatgcaa cgacagcaca gaaagggatc gttcagctca
     1201 gcagcgcgac caacagcaca tctgaaatgc tggcggcaac gccaaagtcg gtaaaggcag
     1261 cctatgacct tgctaacggg aaatataccg ctcaggacgc tacgacagca caaaaaggaa
     1321 ttgtccagct cagtagtgca accaacagcg catctgaaac gcttgccgag acaccgaaag
     1381 cagtgaaagc agctaatgat aatgcgaatg gtcgggtacc ttctgcccgt aaggtgaatg
     1441 gtaaggcgct ttcagcggat ataacactga cgccgaaaga tattggtacg cttaactcaa
     1501 caacaatgtc attcagcggt ggtgctggtt ggttcaaatt agcaacggta accatgccac
     1561 aggcgagttc tgttgtttc
//
LOCUS       DAZGJW010000409         1579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZGJW010000409 DAZGJW010000000
VERSION     DAZGJW010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.413"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            164..226
                     /gene="yoeI"
                     /locus_tag="ACHKIK_005473"
     CDS             164..226
                     /gene="yoeI"
                     /locus_tag="ACHKIK_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO9086393.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            216..1574
                     /gene="plaP"
                     /locus_tag="ACHKIK_005474"
     CDS             216..1574
                     /gene="plaP"
                     /locus_tag="ACHKIK_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO9086394.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
BASE COUNT          325 a          387 c          418 g          449 t
ORIGIN      
        1 taggcacaat gcgccgctgt caaaaaatga ctaaaaaccg acgtttaatt agcgtcggtt
       61 attttttgct tcaaaccaat cattcatacc aagaggccgg gcttcgtacc ggatagatat
      121 ttactaaaaa tcgacagttg ttgtcgctga ggaatccaaa aaaatggggc aattttttgc
      181 ttacgcgacg gttatcaccg taaaggagaa tgaccatgtc gcataacgtt actccaaaca
      241 cctctcgcgt ggaattgcgt aaaacgctta cgttagttcc ggttgtaatg atgggtcttg
      301 cctatatgca gccgatgacg ctgtttgata catttggtat cgtttcaggc ctcacggatg
      361 gtcatgtgcc gacagcctat gcgttcgcat tgattgcgat cctgtttacg gctctgagct
      421 acgggaagct ggttcgccgc tatccttctg ctggctctgc atacacttac gcccagaaat
      481 ccattagccc gactgtcggc tttatggtgg gttggtcttc tctgctcgac tatctgttcg
      541 cgccgatgat caacattctg ctggcgaaaa tctattttga agctctggtg ccttccatcc
      601 catcgtggat gtttgtggtg gcgctggtgg cctttatgac cgcctttaac ctgcgtagtc
      661 tgaaatccgt agcgaacttc aacactgtaa tcgtcgtgtt gcaggtagtg ctgatcgcgg
      721 tgattctggg catggttgtt tatggcgtat ttgaaggtga aggcgcgggt acgctggcga
      781 gcactcgtcc gttctggtct ggcgatgcgc atgttatccc gatgattacc ggggcgacaa
      841 tcctgtgctt ctcctttacc ggctttgacg gcatcagcaa cctgtcggaa gaaaccaaag
      901 atgcagagcg cgtgatcccg cgtgcgattt tcctgaccgc actgattggc ggcatgatct
      961 tcatctttgc aacttacttc ctgcagctgt acttcccgga tatctctcgc tttaaagatc
     1021 cggatgcgtc acagcctgaa atcatgctgt acgttgcggg taaagcgttc caggttggcg
     1081 cgctgatctt ctccaccatt accgtactgg cgtccggtat ggcggcgcat gcaggcgtag
     1141 cgcgtctgat gtacgtaatg gggcgtgacg gtgtgttccc gaaaagcttc ttcggttatg
     1201 tacacccgaa atggcgtact ccggcaatga acatcatcct ggttggcgcg attgccttgc
     1261 tggcaatcaa cttcgacctg gtaatggcta cggcgctgat taactttggt gcgctggtgg
     1321 cgttcacctt cgttaacctg tcggttattt cgcagttctg gatccgtgag aagcgtaaca
     1381 agacgctgaa agatcacttc cagtatctgt tcctgccgat gtgtggtgcg ctgaccgttg
     1441 gtgcgctgtg ggttaacctg gaagaaagct caatggttct gggtctgatc tgggctgcta
     1501 tcggtctgat ttacctggct tgcgtaacca agagtttccg taatccggtt ccgcagtacg
     1561 aagacgtagc ataatcatc
//
LOCUS       DAZGJW010000410         1577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZGJW010000410 DAZGJW010000000
VERSION     DAZGJW010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.414"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..288
                     /locus_tag="ACHKIK_005475"
     CDS             <1..288
                     /locus_tag="ACHKIK_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO9086395.1"
                     /translation="TPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMP
                     ASAAYRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
     gene            541..1284
                     /locus_tag="ACHKIK_005476"
     CDS             541..1284
                     /locus_tag="ACHKIK_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086396.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLST
                     SFNAGSKRFVIYQSAQDKNLQVDIVRTRI"
     gene            1370..1528
                     /locus_tag="ACHKIK_005477"
     CDS             1370..1528
                     /locus_tag="ACHKIK_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO9086397.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
BASE COUNT          402 a          255 c          375 g          545 t
ORIGIN      
        1 acgccgggcg caagatgcag agattgccac ggtacaggcc gtgcggttga tatagccaaa
       61 acagagcagt gggggatagt tgctgagaaa gagtgcggaa gatgtaaagg cgtcggttat
      121 tcaagaatgc cagcaagcgc cgcatatcgc gctgtgacga tgctaatccc aaaccttacc
      181 caacccacct ggtcacgcac tgttaagccg ctgtatgacg ctctggttgt gcaatgccac
      241 aaggaagagt caatcgcaga caacattttg aatgcgatca cacgttagcg ccatgattgc
      301 cacggatggc aacatattaa cggcataata ttgacttttt gaataacttt ggggaaactt
      361 gacaccaata atgggcgttt tttacatgtc attgatgagt ctcaataacc tgccgccgag
      421 tagtttttat gctctgaatt gtatttgtgt agtaaacatg ctgactgcaa tgtaatagag
      481 tttttttagc ctgtaacctc ttgacggcat tgaattgctt ttgttatgag ttgtaagcca
      541 atgttatcat cttgtattgg ggtggttatg aaggatggtg cgctgctcag gagttcttca
      601 ctttttattg cctacatggg atgccttgga tgggggagtg cttatttcta tggatggggt
      661 acttcttttt actacggctt cccatggtgg attgtaggtg caggtgttga tgatgttgcc
      721 agaagtttat tttttgcagt tatcgtcatt gctatatttc ttatcggttg gggtattggt
      781 gttgtattct ttttcgcagt gaaaagaaaa cattctatgc aagagctaaa tgtatttcgc
      841 ctttattttg ctgtggaatt attgtttgtg ccggcaatta ttgagttttc tatattgaga
      901 cagaagattc aggtacctct tttgctactg tcagcagcga ttgcgctggc ggttacaatt
      961 tcgataagat cttatgggcg atttttatcg gtatcatgct tctatgataa gccatttata
     1021 aaaaaacatt tttttgagat tgtgatgatt gcttttgtgg catatttttg gcttttttca
     1081 tttctgacag gatattacaa accacagttt aagaaagaat atgaaatgat taattataat
     1141 gatggttggt attatgttct tgctcgttat gataattgtc tggttttgtc tacttctttc
     1201 aatgcaggta gtaaaaggtt tgtcatttat caatcagcac aagataagaa tcttcaggtt
     1261 gatattgtaa ggaccagaat ttaattggct gcataaataa tattttaagt tgcaagttgg
     1321 ctattcgtag gaatagaacc ttaggcatgc tgaatgcgtt ttctgaacat tgttttataa
     1381 actgtgtctg cttgctgttg tgatcctgct tttagtgatg gtgatgatgg atttcaccag
     1441 caggataatg ttggtactga ctgatggcgc tctggtctgc ggcattgtgg tattgctgtg
     1501 gccgatgatg aaagaacaga atgaataatt cttgactttt ttgtttactg tttattaaaa
     1561 aatcaaccgc atggtga
//
LOCUS       DAZGJW010000411         1568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZGJW010000411 DAZGJW010000000
VERSION     DAZGJW010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.415"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..471
                     /locus_tag="ACHKIK_005478"
     CDS             <1..471
                     /locus_tag="ACHKIK_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO9086398.1"
                     /translation="QGKVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLG
                     MVAKDDVVILISNSGETAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVK
                     SEADNLGLAPSCSSTVVLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            543..1490
                     /locus_tag="ACHKIK_005479"
     CDS             543..1490
                     /locus_tag="ACHKIK_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO9086399.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
BASE COUNT          426 a          302 c          377 g          463 t
ORIGIN      
        1 cagggaaaag ttgtttttat tggtgttggg aaatctggca ttattgccag aaaacttgct
       61 gcaacatttg ccagcaccgg aactccctcg ttttttgttc acggtacgga agcggtacac
      121 ggcgaccttg ggatggtggc gaaagacgat gttgttattc ttatttcaaa cagtggtgag
      181 acggcggaaa tcctggcgac actgcctagt ttgaaaaaaa tgggtaatta tttgatctct
      241 tttacccgca gtcatcactc atcgcttgcg ataagttgtg atttatctgt tgaaatcccc
      301 gtcaaaagtg aggcggataa tttaggttta gcaccgtcat gctcgtctac tgttgttctg
      361 gttgttggtg atgcggtggc gcttgcactc tctgaattga aaaaatttac tcgtgctgat
      421 ttcggcttat atcatccagg aggtgcactc ggcattaaag caaattcata atttcaaagt
      481 ataaatacag taaccggtca cgccatcttt tggggtggcg ttcattgaag tgagtgtcag
      541 ctatgaaaaa tgttctggtc accgtcccca gcttttcagc acgctgtgtt tccgccagca
      601 agttgctaag agagaataat tttaatctta ttattaagaa taatgtggag catctgctta
      661 aatcagaatc cactgcatta cgagaatcta tttgcgcggt catcgccgga aaagattgtt
      721 atcaggcgga cacgctatca ttgctgcctg gtgtgaaaat aatatcgaga ttcgggacgg
      781 ggatagataa tattgatctt cgtgcagcac aacaatccgg tattgtagtc aataatgcgg
      841 ttgggattaa ttcaaatgct gtcgcggaat ttatcattgg acttatcttc gcgagcatga
      901 gaaatattcc aggcagctat catgcgatgc aaaatggcta ctggggtgag tcgcatggct
      961 atgaattaca aggcaaacgg attggcatgg ttggctacgg caatataggt aaaaccctgg
     1021 caaaaaggtt atccggtttt gatgttgagc ttttagctta tgacaaacaa cccgattatc
     1081 aggttgctga caaagttgga gttcagtttg tatcaattga agatatcttt atgcagtcgc
     1141 atgtcatcat tgttcttttg cctttttctc ctgagctgga gaacttcatt agtcataaat
     1201 atttgtcgat gatgcgcaat ggtgctctaa tcatcaatgc cgcaagggga aagttgctgg
     1261 atgagggcgc tttactacag gtgattgaag aacgaaatgt gtttgcggcg ctggatgtgt
     1321 ttagctctga accgttggcg caatttagcc cactgctaca tgccaaaaat atcattacaa
     1381 cgccgcatat tgcggccgcc accgttgagt cataccagca aaccggtaca cacgttgccc
     1441 agtcgataat tgattacttc gcaggaaggg agataaaaaa cgtgttgtga gttcagcgaa
     1501 cgaagttttt aaatacaaaa aaaaccgcaa tttgtggaac acattgcggt ttatcagcat
     1561 tctaagta
//
LOCUS       DAZGJW010000412         1568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZGJW010000412 DAZGJW010000000
VERSION     DAZGJW010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.416"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..348
                     /locus_tag="ACHKIK_005480"
     CDS             <1..348
                     /locus_tag="ACHKIK_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO9086400.1"
                     /translation="VFDRIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTA
                     DILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRA
                     LWVMAETLLESMR"
     gene            348..593
                     /locus_tag="ACHKIK_005481"
     CDS             348..593
                     /locus_tag="ACHKIK_005481"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086401.1"
                     /translation="MKHIPEGVIQVPESPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     EISLCRLSTGNDIWPSVCDRNGYAYGCPEFQPNIIHP"
     gene            687..1160
                     /locus_tag="ACHKIK_005482"
     CDS             687..1160
                     /locus_tag="ACHKIK_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9086402.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYSLSNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            1176..>1568
                     /locus_tag="ACHKIK_005483"
     CDS             1176..>1568
                     /locus_tag="ACHKIK_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFO9086403.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQAL"
BASE COUNT          353 a          453 c          426 g          336 t
ORIGIN      
        1 gtttttgacc ggattgagga aaagcgtgat gccatgcagt cgctaattct gccgccaccg
       61 gcacgccagg cgctggcaca ggcggcactg acttaccgtt atggtgacga acatcagccc
      121 gtcaccaccg ccgacattct gacgccacga cgccgggagg attacggtaa ggacctgtgg
      181 agtgcttatc agaccatcca ggagaatatg ctgaaaggcg ggatttccgg tcgcagtgca
      241 aaaggaaaac gtatccacac ccgtgccatt cacagcatcg acaccgatat taagctcaac
      301 cgcgcattgt gggtgatggc agaaacgctg ctggagagta tgcgctgatg aaacatatcc
      361 ctgaaggcgt gattcaggta ccagaatccc ccgacgcccc ggaagcgctg ccggggtgtc
      421 gttccatttc tgatgatgac ctgtgcgcct ggtgtacccg tctgctttac cgtcccggag
      481 aaatcagtct gtgcagactg agtacaggca atgacatctg gccatccgtc tgtgaccgga
      541 acggttacgc gtacggctgt ccggaatttc agccgaatat cattcatccc tgatattacc
      601 gggtctcacg gtcagtgatg aacgtgatgt tgccagctcc cgacctttac tccctctcat
      661 ttattcacat tacaaggatt tcatacatga aaacattgtc tcagaacacc acctcttcgg
      721 cctgtgcacc ggagactggc ctgcaacaac tggttgccac catagtccct gatgaacagc
      781 gcatcagctt ctggccgcag cactttggtc tcattccaca gtgggtgacc ctggagcccc
      841 gtgtcttcgg ctggatggac cgtctgtgcg aagactactg cggtggtatc tggaatctgt
      901 acagtctgag caacggcggg gcatttatgg cccccgaacc ggatgacgat gatgacgaaa
      961 catgggtact gttcaatgcc atgaacggta accgcgctga aatgagcccg gaagccgctg
     1021 gcattgctgc ctgtctgatg acgtacagtc atcacgcctg tcgtacggag tgttatgcca
     1081 tgacggtcca ttattaccgg ctgcgggatt acgccctgca gcatccggaa tgcagcgcca
     1141 ttatgcgcat catcgactga atggaggcag ggacaatgca gcagctttcc tttctgcccg
     1201 gagaaatgac gcccggcgag cgcagtctca ttctgcgggc tctgaaaacc ctggaccgcc
     1261 atcttcatga acccggtgtg gccttcacct ccacccgtgc ggcacgggaa tggctgattc
     1321 tgaacatggc gggactggag cgtgaagagt tccgggtgct gtatctgaat aaccagaatc
     1381 agctgattgc cggtgaaacc ctcttcaccg gcaccatcaa ccgcacggaa gtccatcccc
     1441 gggaagtgat taaacgcgcc ctgtaccaca atgccgctgc cgtggtgctg gcgcacaatc
     1501 acccgtccgg tgaagtcaca cccagcaagg cagaccgcct tatcacggaa cgtctggtac
     1561 aggcactg
//
LOCUS       DAZGJW010000413         1563 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZGJW010000413 DAZGJW010000000
VERSION     DAZGJW010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.417"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..615
                     /locus_tag="ACHKIK_005484"
     CDS             <1..615
                     /locus_tag="ACHKIK_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO9086404.1"
                     /translation="TRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIILLNSHDGTSSY
                     QMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFDRIEEKRDAMQ
                     SLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWSAYQTIQENML
                     KGGISGRSAKGKRIHTRAIHSIDTDIKLNRVLWVMAETLLESLR"
     gene            670..1155
                     /locus_tag="ACHKIK_005485"
     CDS             670..1155
                     /locus_tag="ACHKIK_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9086405.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            1171..>1563
                     /locus_tag="ACHKIK_005486"
     CDS             1171..>1563
                     /locus_tag="ACHKIK_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFO9086406.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQAL"
BASE COUNT          358 a          441 c          432 g          332 t
ORIGIN      
        1 acccgtgtgc gcgacccggg ccgccgggga tacacaaaac acatgctgcg tctgcggcgg
       61 gccggagaga taaacggaga acatgtccct gaaattattc tgctcaactc tcatgacggt
      121 acctccagct accagatgct gccgggttac ttcaggttcg tctgccagaa cggatgcgtc
      181 tgtggtcagt ctctggggga agtgcgtgtt ccgcaccggg gaaatgtggt ggacagagtc
      241 attgaagggg cttacgaggt ggtgggcgtg tttgaccgga ttgaggaaaa gcgtgatgcc
      301 atgcagtcgc tgattctgcc gccaccggca cgccaggcgc tggcacaggc ggcactgact
      361 taccgttatg gtgacgaaca tcagcccgtc accaccgccg acattctgac gccacgacgc
      421 cgggaggatt acggtaagga cctgtggagt gcttatcaga ccattcagga gaatatgctg
      481 aaaggcggaa tttcaggtcg cagcgctaaa ggaaaacgta tccatacccg tgccattcac
      541 agcattgaca ctgacattaa gctcaaccgc gtattgtggg tgatggcgga aaccctgctg
      601 gagagcctgc gctgattcag ccattaccct gaaaaccacg catttatttg tcctttaagg
      661 agtcatttca tgaaaaccat ttcacataat tccaccacat cttctgtttc cgtaaccgct
      721 gcatcaggga atgaccagcc tcagttggtt gccaccctcg tccctgatga acagcgcatc
      781 agcttctggc cgcagcactt tggcctcatt ccacagtggg tgaccctgga gccccgtgtc
      841 ttcggctgga tggaccgtct gtgtgaagac tactgcggtg gtatctggaa tctgtacacc
      901 ctgaacaacg gcggggcatt tatggcaccc gaaccggatg acgatgatga cgaaacatgg
      961 gtactgttca atgccatgaa cggtaaccgc gctgaaatga gtccggaagc tgccggcatt
     1021 gctgcctgtc tgatgacgta cagtcatcat gcctgtcgta cggagtgtta tgccatgacg
     1081 gtccattatt accggctgcg ggattacgcc ctgcagcatc cggaatgcag cgccattatg
     1141 cgcatcatcg actgaatgga gagaagtaca atgcaacagc tttcctttct gcccggagag
     1201 atgacgcccg gcgagcgcag cctcattcaa cgggccctga aaaccctgga ccgccatctt
     1261 catgaacccg gcgtggcctt cacctccacc cgtgcggcac gagaatggct gattctgaac
     1321 atggcgggac tggagcgtga agagttccgg gtgctgtatc tgaacaacca gaatcagctg
     1381 attgccggtg aaaccctctt caccggcacc atcaaccgca cggaagtcca tccccgtgaa
     1441 gtgattaaac gcgccctgta tcacaatgcc gctgctgtgg tgctggcaca caatcacccg
     1501 tccggtgaag tcacacccag caaggcagac cgccttatca cggaacgtct ggtacaggca
     1561 ctg
//
LOCUS       DAZGJW010000414         1553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZGJW010000414 DAZGJW010000000
VERSION     DAZGJW010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.418"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..111)
                     /locus_tag="ACHKIK_005487"
     CDS             complement(<1..111)
                     /locus_tag="ACHKIK_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO9086407.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNT"
     gene            complement(263..862)
                     /locus_tag="ACHKIK_005488"
     CDS             complement(263..862)
                     /locus_tag="ACHKIK_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO9086408.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFMDALGLITSFSYANAEDDPKTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYDGSGSGDWRSDAFIVGIGYRF"
     gene            complement(930..>1553)
                     /locus_tag="ACHKIK_005489"
     CDS             complement(930..>1553)
                     /locus_tag="ACHKIK_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086409.1"
                     /translation="MNEVFLKYLTAPTITSGGNPPAFSLTPDGRLTAKNADISGNVNA
                     NSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYD
                     DQPFDRQIVIPAVAFSGAKHEKEHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVI
                     DMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATAGITIS"
BASE COUNT          375 a          439 c          361 g          378 t
ORIGIN      
        1 cgtgttcacc accaccgtgc tgctggttcg tctggccttc agcacaatgg tgcagttctg
       61 tactggtttt cctgtgccat ctttaagcac gccagaaatt tttactgtca tacttttcca
      121 ccaataaaaa aagcccgcag cagtgacgcc acgggcttca ggacagtgta actttacgtt
      181 tcctcaaacg cagttcaccc cataaggtgg atgaacctgc gtatcataac aatatttaca
      241 gaagataaat cggcgtctgt tgtcagaaac ggtatccgat accaacaata aatgcatccg
      301 atcgccagtc gccactaccg gaaccgtcat aagcaaggtc aatggtcacg gattcggtcg
      361 ggttaaactg cacgccagcc ccccacgcca gagacgtgtt gctgtggcga ccgtcatcac
      421 ttccggtcag cacatcgtgc gtttttccct tgttgtcagt tacgcggaga taatccccgg
      481 agaaagtcga cacacggctg taagccacac ccgccatcgc atacgcgctg aaccattcat
      541 tcacgcgtac agacggcccc gccatcacgc tgaaccagcg gttacgcacg gaatcttcat
      601 gccagcgggt atcgctgtag cgcgtttttg ggtcatcctc agcattggca taactgaagg
      661 acgtaatcag ccccagcgcg tccataaact cataacggta tttcacgtta atcccgttca
      721 gatcatcact accgggaacg ttcgtcgagg catggagata ccccgcgctc agcgtggact
      781 gatgttctgc tgcactcgct ggcgtagcag cggcgacctg ccagactact gcggacaaaa
      841 taacagcaca taatttacgc ataattacct ctcgcttttc tgcaataaaa aaggcgccat
      901 ttctggcgcc cgtatctggg ttataaaatt cagctaatcg tgatgcctgc agtggctttc
      961 ttcatcacca caaccagcaa atcgctgata cttgctgtgg gataccagtt atttaccagc
     1021 catgctgaca ccgaaaactc cagtgtcatg tgaccgtgac cggcaggcat atcaataaca
     1081 ccactgtaaa tcagcgtatt atccagcgcg gtacggttat aaatttcagc accgtttttc
     1141 cgcactatca gacggcatga ggagtaaata tcagtatgct ctttctcatg tttagcgccg
     1201 ctgaatgcca ccgccggaat aacaatctgc cggtcaaacg gctgatcgtc ataaaccctg
     1261 acggtaatgg ttcctgatgg ccaccgctcc ggtgcacggg agtcccgggg gaaagctttg
     1321 cccactgttt taacgagatc gccttcaatc tggttcgcgg acaattttcc cagaacccga
     1381 cagttctcgt taatcgtgac gttgttgagc gtcccggagt tcgcattcac gttaccgctg
     1441 atatcggcat ttttcgccgt cagccgcccg tccggtgtca gggaaaatgc cggaggatta
     1501 ccgccgctgg taatggtggg agccgtcaga tatttcagga acacttcatt cat
//
LOCUS       DAZGJW010000415         1547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZGJW010000415 DAZGJW010000000
VERSION     DAZGJW010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.419"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..724
                     /locus_tag="ACHKIK_005490"
     CDS             <1..724
                     /locus_tag="ACHKIK_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO9086410.1"
                     /translation="AGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIV
                     ALGTRHSMKASTDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDG
                     KDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVSELPVNWWV
                     QPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGY
                     AHSVSGSSAEGYNGQATLNVTF"
     gene            845..>1547
                     /locus_tag="ACHKIK_005491"
     CDS             845..>1547
                     /locus_tag="ACHKIK_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YeeR"
                     /protein_id="HFO9086411.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLT"
BASE COUNT          332 a          425 c          428 g          362 t
ORIGIN      
        1 tgctggccat tcttccgttg atgttaagga tgatgacggc tcccgtgccg gcacggtccg
       61 ggatgatgcc ggcagcctgg gcggatacct gaatctgata cacaacgcct ccggactgtg
      121 ggctgacatt gtggccctgg gaacccgcca cagcatgaaa gcgtcaacgg acaataacga
      181 cttccgcgcc cggggttggg gctggctggg ttcactggaa accggtctgc ccttcagtat
      241 cactgacaac ctgatgctgg agccacaact gcagtatacc tggcagggac tttccctgga
      301 tgacggtaag gacaacgccg gttatgtgaa gttcgggcat ggcagtgcac aacatgtgcg
      361 tgccggtttc cgtctgggca gccacaacga tatgaccttt ggcgaaggca cctcatcccg
      421 tgcccccctg cgtgacagtg caaaacacag tgtgagtgaa ttaccggtga actggtgggt
      481 acagccttct gttatccgca ccttcagctc ccggggagat atgcgtgtgg ggacttccac
      541 tgcaggcagc gggatgacgt tctctccctc acagaatggc acatcactgg acctgcaggc
      601 cggactggaa gcccgtgtcc gggaaaatat caccctgggc gttcaggccg gttatgccca
      661 cagcgtcagc ggcagcagcg ctgaagggta taacggtcag gccacactga atgtgacctt
      721 ctgacagaac catcgcctct ctgtggtccc ggtcatcatg accgggaccc ggaccggcgc
      781 aacggatctt caacgccaca ttcgctggca ttaacaataa catgatattc atcacggagt
      841 gactatgtta cagatagtcg gcgcgctgat cctgctgatc gcaggatttg ccattcttcg
      901 ccttttgttc agagcattaa tcagcacggc ttctgcgctg gcagggctca tattgctgtg
      961 tctgttcggc ccggccttac tggctggcta tatcaccgaa cgcataaccc ggttgttcca
     1021 tattcgctgg ctggcaggcg tatttctgac gattgccgga atgatcatca gcttcatgtg
     1081 gggacttgat ggtaaacata tcgcgctgga ggctcacacc tttgactctg tgaaatttat
     1141 tctgaccacc gctctcgccg gtggtctgct ggctgttccc ctgcagatca aaaacattca
     1201 gcagaacggg atcacaccag aagatatcag caaggaaatt aacgggtatt actgctgttt
     1261 ttatactgcc tttttcctta tggcgtgttc tgcatgcgca ccattgatcg cgttacagta
     1321 cgatatttca ccgtcactga tgtggtgggg cgggttgttg tactggctgg ctgcattagt
     1381 gacgctgcta tgggcggcca gccagatcca ggcgctgaaa aaactgacct gtgccatcag
     1441 ccagacactg gaagaacaac cggtgctcaa cagtaaatcg tggctgacca gtttgcaaaa
     1501 cgattacagc cttcctgact cactgacgga gcgcatctgg ctgacgc
//
LOCUS       DAZGJW010000416         1544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZGJW010000416 DAZGJW010000000
VERSION     DAZGJW010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.420"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..356)
                     /locus_tag="ACHKIK_005492"
     CDS             complement(<1..356)
                     /locus_tag="ACHKIK_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9086412.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVP"
     gene            484..1344
                     /locus_tag="ACHKIK_005493"
     CDS             484..1344
                     /locus_tag="ACHKIK_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9086413.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNMKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQIEDIISVINWAEKQACIDNQRIG
                     LWGTSLGGCHVFSAAAQDQRVKCIVSQLAFADGDALITGEMNELERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLNDDESKVFFEKVKAQHPEMDIKIPFLTVMETLQYKPAESAA
                     RVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGTFFERVAA
                     VQTQWFKKYI"
BASE COUNT          420 a          303 c          405 g          416 t
ORIGIN      
        1 ggaaccagac ggacttcatc ttttggcggg cgtccgcgtc gcgcgcgcac tttttcggac
       61 aggatccgca caaacggtct tacaccatcc agatcaaatg attccggtgt cagcatccgg
      121 gcttcgccaa tacgcattcc ggtattccag agggtggcga acagcatatg gtgacgctga
      181 tccggcatat agaaaagaag ggcactcact tccggggcca gcaggtaggc cggtgtggcc
      241 cctgtggaag tggccattct tctcagggag agcgctgttg caaaatccac ccccggcgca
      301 atgggtaaca gggagacgcg ttctggtgaa ttctgcaggg gaatgacatt gttcatgagt
      361 tatttttcac gtagcacacg cactgtttat ggatactgat tgcgctgggc attctggtat
      421 tgtgactggt atgatgctgg cgctgattct gatgattaat aaacaacata acacaggata
      481 tttatgaaaa ttacggatca caaactttct gaaggtattg ctctgacatt tcgtgttcct
      541 gaaggaaata tgaaacatcc tttgattatc ttatgccatg gattttgcgg tatccggaat
      601 gttctgctcc cttgctttgc gaatgctttc acagaagcag ggttcgcaac gattactttt
      661 gattatcgtg gattcggtga gagtgaagga gaacggggcc gtctggttcc agcaatgcag
      721 atcgaagata ttatttcagt tatcaattgg gcagaaaagc aggcatgtat tgataatcag
      781 cgtatcgggt tatggggaac atcgctgggt ggttgtcatg tgttcagtgc agctgcgcaa
      841 gatcaaagag tgaaatgtat tgtcagccag ctggcttttg ctgatggtga tgctctgatt
      901 accggagaga tgaatgaatt ggagagagct tcttttctgt ccacactgaa taaaatggca
      961 gagaaaaaga aaaacacagg gaaggagatg tttgtggggg ttacacgggt actaaatgat
     1021 gatgagtcga aggtattctt cgagaaagtc aaagctcagc atcccgaaat ggatataaaa
     1081 atacctttcc ttactgtcat ggaaacgttg caatataaac cggccgagag cgcagccaga
     1141 gtccagtgtc cagtattggt tgttattgct ggccaggata gtgttaatcc accggaacag
     1201 ggaagagcat tatatgatgc tgttgcttct ggtactaaag aattatatga ggaagctgac
     1261 gcatgccatt atgatattta tgagggcaca ttttttgagc gggtagcagc tgtgcaaaca
     1321 caatggttta aaaaatatat ttaatagtga gcctgtgttt accattctgt gtaaatactt
     1381 tttcttaaaa gtgaccgtca aggcggtcac taaactttat aataaagagg catcgttcat
     1441 ttccgggagg cagactggat tgccagcccc ccacttttta ccaagctgca ggtatggggg
     1501 acacggacac tggtgttcac agaaaaaaat aaccatctct gaaa
//
LOCUS       DAZGJW010000417         1543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZGJW010000417 DAZGJW010000000
VERSION     DAZGJW010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.421"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..643)
                     /locus_tag="ACHKIK_005494"
     CDS             complement(<1..643)
                     /locus_tag="ACHKIK_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9086414.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKF"
     gene            complement(643..972)
                     /locus_tag="ACHKIK_005495"
     CDS             complement(643..972)
                     /locus_tag="ACHKIK_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9086415.1"
                     /translation="MKTFRWKVKPGMDVTSAPSVREVRFGDGYSQRVPAGLNADLKTY
                     SVTLSVSREEATALESFLAEHGGWKAFLWTPPYGYRQIKVTCAKWSSQVSMLRVEFSA
                     EFKQVVN"
     gene            complement(969..>1543)
                     /locus_tag="ACHKIK_005496"
     CDS             complement(969..>1543)
                     /locus_tag="ACHKIK_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9086416.1"
                     /translation="QNMAAMLTGSEQNWRSFTRSVLSMMTEILLKQAMVGIVGSIGSA
                     IGGAVGGGASASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSR
                     IGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFEQNNHVVINNDGTNGQIGPAAL
                     KAVYDMARKGARDEIQTQMRDGGLFSGGGR"
BASE COUNT          321 a          518 c          375 g          329 t
ORIGIN      
        1 ggaacttaca accgctcagg catttgctgc atttatcctt cgtgatatcg gacgttggct
       61 ggtcatattc atccgcgaca gccggaccgc tataaccgca ctcgtcaccg cgataggtcc
      121 aggtgcaggt gttggccagc atgatacgtc ccggaaaaac agcgccatcc gtttccgtcg
      181 gcgtggacag tacaaaggag gcactcaccg cgctcagttc gctgcactgc tcgatgcgcc
      241 agcggctgat cacctcctgc tccggatcgg cgtcactgtt tccgttgacg aagttcaccg
      301 catccagaaa acgggcgtaa accttacgcc ggaccaccgt tccgccgacc agactctgca
      361 gatcttccgc catcccggtg accataccgt acaggttaga aaccgtcagc gtggggcgcg
      421 tactggtgcc tttgccattc agttcaaaac cgctcccctg aatgggatac ggctgatact
      481 gtcgcccctg ccaggtgacc ggctcacctt tttcgttctg ctcattacag aaaaaataac
      541 gttcaccacc gacctctgtc agatcgattt cccagagcac cacgctggcc gactgctccg
      601 cacgggtgca ttcattcagt gtttcctgcc ggatatcctg catcagttca ccacctgttt
      661 aaactctgcg ctgaactcaa cacgcaacat actgacctgc gacgaccatt ttgcgcaggt
      721 cacctttatc tgcctgtaac cataaggcgg cgtccacaga aaggccttcc agcccccgtg
      781 ctcagccaga aacgactcca gcgccgtggc ctcctcacgg gagacagaca gcgtcacgct
      841 gtacgttttc aggtcagcgt tcagcccggc aggcacacgc tgggaatagc catcaccaaa
      901 gcgcacctcc ctgacggaag gggccgatgt cacatccata ccgggtttca ctttccagcg
      961 gaaggttttc atcgtccacc tccggagaac aggccaccat cacgcatctg tgtctgaatt
     1021 tcatcacggg cacccttgcg ggccatgtca tacaccgcct tcagagcagc cggacctatc
     1081 tgcccgttcg tgccgtcgtt gttaatcacc acatggttat tctgctcaaa cgtcccggac
     1141 gcctgcgacc ggctgtctgc catgctgccc ggtgtaccga cataaccgcc ggtggcatag
     1201 ccgcgcatca gccggtaaag attccccacg ccaatccggc tggttgcctc cttcgtgaag
     1261 acaaactcac cacggtgaac aatccccgct ggctcatatt tgccgccggt tcccgtaaat
     1321 cctccggttg caaaatggaa tttcgccgca gcggcctgaa tggctgtacc gcctgacgcg
     1381 gatgcgccgc caccaacagc cccgccaatg gcgctgccga tactcccgac aatccccacc
     1441 attgcctgct taagcagaat ttctgtcatc atggacagca cggaacgggt gaagctgcgc
     1501 cagttctgct cactgccggt cagcatcgcc gccatattct gtg
//
LOCUS       DAZGJW010000418         1541 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZGJW010000418 DAZGJW010000000
VERSION     DAZGJW010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.422"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1128
                     /gene="gltS"
                     /locus_tag="ACHKIK_005497"
     CDS             <1..1128
                     /gene="gltS"
                     /locus_tag="ACHKIK_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO9086417.1"
                     /translation="PFLQKNHIPEAVVGGFIVAIVLLIIDKTSGYSFTFDASLQSLLM
                     LTFFSSIGLSSDFSRLIKGGKPLVLLTIAVTILIAIQNTVGMSMAVMMNESPFIGLIA
                     GSITLTGGHGNAGAWGPILADKYGVTGAVELAMACATLGLVLGGLVGGPVARHLLKKV
                     SIPKTTEQERDTIVEAFEQPSVKRKINANNVIETISMLIICIVVGGYISALFKDTFLQ
                     LPTFVWCLFVGIIIRNTLTHVFKHEVFEPTVDVLGSVALSLFLAMALMSLKFGQLASM
                     AGPVLIIIAVQTVVMVLFACFVTFKMMGKDYDAVVISAGHCGFGMGATPTAIANMQTV
                     TKAFGPSHKAFLVVPMVGAFIVDISNSILIKIFIEIGTYFT"
     gene            1359..1478
                     /locus_tag="ACHKIK_005498"
                     /pseudo
     CDS             1359..1478
                     /locus_tag="ACHKIK_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227464.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT          425 a          297 c          308 g          511 t
ORIGIN      
        1 ccgttcctac aaaaaaacca cataccagaa gccgtcgttg gtggctttat tgttgcaatt
       61 gttctgttaa ttattgataa aacatcaggt tattcgttta cttttgatgc ttcattgcaa
      121 agtttattaa tgctcacatt cttttcctct atcgggctaa gttctgactt ttctcgactg
      181 attaaaggag gaaagccgtt agttctatta actattgcag taacgatcct aatcgccatt
      241 caaaatactg tcggcatgag tatggctgtc atgatgaatg aaagtccatt tattggctta
      301 attgcaggtt caattactct aacaggtggt catggtaatg ccggagcatg gggccctatt
      361 ctcgctgata aatatggtgt aacaggcgcc gttgaattag cgatggcttg tgcaacactt
      421 ggattagtgt tgggtggttt agttggcggc cctgttgccc gtcatctttt gaaaaaggtc
      481 tctattccta aaacaaccga gcaagagcgc gacactatcg ttgaagcttt tgagcaacca
      541 agcgtcaaaa gaaaaatcaa tgcaaataac gttattgaaa ccatttcaat gctgattatc
      601 tgtattgttg ttggtggcta tatcagtgca ttgtttaaag atacttttct gcaactgcct
      661 acttttgtct ggtgtttatt tgtcggtatt attatccgta atacactgac tcatgtattt
      721 aaacacgaag tgtttgagcc gaccgtcgat gtattaggta gcgttgcttt atcgcttttc
      781 ttggcaatgg cgttaatgtc attaaaattt ggtcaattgg caagcatggc agggccagta
      841 ttaattatca ttgctgtaca aactgttgtc atggtgctat ttgcctgctt tgtcaccttc
      901 aaaatgatgg gcaaagatta tgatgctgtc gtgatcagcg cgggtcactg tggctttggt
      961 atgggagcaa caccaacagc aattgcgaat atgcaaacag tcacaaaagc atttggacca
     1021 tcacataaag ctttccttgt cgttcctatg gtcggtgcct ttattgttga tatttcaaac
     1081 agtatcttaa ttaaaatatt tattgaaatt ggtacatact ttacctaata gctacacact
     1141 actcggcgta cttctcctaa caatttatta tagggcagta tgccgagccg ctttcttacc
     1201 ccgtatattc ccctagtcaa tctatttagc ttgcacgttt acgaattaat tctcgtgaca
     1261 actaaaccaa aatttgaatt atgacgagta tatctgatta tttatacttt attcgttaga
     1321 atagtccgta gaaataaata atcagtaggc tacatggaat gaaattaaga catctcgata
     1381 tcttttatgc tgtaatgact tgtggttcct taacgcgtgc ggctgaagtt ttacatattt
     1441 ctcaacccgc ggcgagtaaa gcactcaaac atgctgagca ctgagagatc ccctcataat
     1501 ttccccaaaa cgtaaccatg tgtgaataga ttttgagtaa g
//
LOCUS       DAZGJW010000419         1539 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZGJW010000419 DAZGJW010000000
VERSION     DAZGJW010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.423"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..639)
                     /locus_tag="ACHKIK_005499"
     CDS             complement(<1..639)
                     /locus_tag="ACHKIK_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9086418.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMVEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCK"
     gene            complement(639..968)
                     /locus_tag="ACHKIK_005500"
     CDS             complement(639..968)
                     /locus_tag="ACHKIK_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9086419.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(965..>1539)
                     /locus_tag="ACHKIK_005501"
     CDS             complement(965..>1539)
                     /locus_tag="ACHKIK_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9086420.1"
                     /translation="QNMAAMLTGSEQNWRSFTRSVLSMMTEILLKQAMVGIVGSIGSA
                     IGGAVGGGASASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSR
                     IGVGNLYRLMRGYATGGYVGTPGSLADSRSQASGTFEQNNHVVINNDGTNGQIGPAAL
                     KAVYDMARKGARDEIQTQMRDGGLFSGGGR"
BASE COUNT          320 a          515 c          369 g          335 t
ORIGIN      
        1 cttacaaccg ctcaggcatt tgctgcattt atccttcgtg atatcggacg ttggctggtc
       61 atattcatcc gcgacagccg gaccgctata accgcactcg tcaccgcgat aggtccaggt
      121 gcaggtgttg gccagcatga tacgtcccgg aaaaacagcg ccatccgttt ccgtcggcgt
      181 ggacagtaca aaggaggcac tcaccgcgct cagttcgctg cactgctcaa tgcgccagcg
      241 gctgatcacc tcctgctccg gatcggcgta actgtttccg ttgacgaagt tcaccgcatc
      301 cagaaaacgg gcgtaaacct tacgcctgac caccgttccg ccgaccagac tctgcatatc
      361 ttccaccata ccggtgacca tgccgtgcag gttagaaacc gtcagtgtcg gacgggcagc
      421 actgcccttg ccgttcagtt caaatcccgt cccctgaatg ggatacgcct gatactgtcg
      481 cccctgccag gtgaccggct cacctttttc gttctgctca ttacagaaaa aataacgttc
      541 tccaccgacc tctgtcaggt cgatttccca gagcaccacg ctggccgact gctccgcacg
      601 ggtgcattca ttcagtgttt cctgccggat atcctgcatc agttcaccac ctgttcaaac
      661 tctgcgctga actcaacacg cagcatactg acccgcgacg accattttgc gcaggtcacc
      721 tttatctgcc gccactcata aggcggcgtc cacagaaagg atttccagcc cccgtgctct
      781 tccagaaacg actccagtac cgtggcctcc tcacggggga cagaaagcgt cacgctgtac
      841 gttttcaggt tggcattcag cccggcaggc gctcgctggg aatagccatc accaaagcgc
      901 acctttctta cagaaggggc cgaagccaca tccataccgg gtttcacttt ccagcggaag
      961 gttttcatcg tccacctccg gagaacaggc caccatcacg catctgtgtc tgaatttcat
     1021 cacgggcacc cttgcgggcc atgtcataca ccgccttcag agcagccgga cctatctgcc
     1081 cgttcgtgcc gtcgttgtta atcaccacat ggttattctg ctcaaacgtc ccggacgcct
     1141 gcgaccggct gtcagccaga ctgcccggtg taccgacata accaccggtg gcatagccgc
     1201 gcatcagccg gtagagattc cccacgccaa tccggctggt tgcctccttc gtgaagacaa
     1261 attcaccacg gtgaacaatc cccgctggct catatttgcc gccggttccc gtaaatcctc
     1321 cggttgcaaa atggaatttc gccgcagcgg cctgaatggc tgtaccgcct gacgcggatg
     1381 cgccgccacc aacagccccg ccaatggcgc tgccgatact cccgacaatc cccaccattg
     1441 cctgcttaag cagaatttct gtcatcatgg acagcacgga acgggtgaag ctgcgccagt
     1501 tctgctcact gccggtcagc atcgctgcca tattctgtg
//
LOCUS       DAZGJW010000420         1528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZGJW010000420 DAZGJW010000000
VERSION     DAZGJW010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.424"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(18..329)
                     /locus_tag="ACHKIK_005502"
     CDS             complement(18..329)
                     /locus_tag="ACHKIK_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFO9086421.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(357..>1528)
                     /locus_tag="ACHKIK_005503"
     CDS             complement(357..>1528)
                     /locus_tag="ACHKIK_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO9086422.1"
                     /translation="NFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSI
                     MIAFLVARNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVA
                     VIVALITSEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTL
                     ISPAGLNDLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFS
                     EANLENLSWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYR
                     DLAKMSFIPGIFNINEPIMFDLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVA
                     YAVPWTTPGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
BASE COUNT          478 a          344 c          324 g          382 t
ORIGIN      
        1 cgcactggag gaaaaaatca ggcatggagt tcttttctta atgaaataat ttccctggct
       61 aaatcctggc ataatattgc attcatcaaa tgatcctgtg catgaatcag aattaaatta
      121 acttttattt tgccacagcc ttcatcctga ctaattaatg cggtctgaat cttatgtgcc
      181 tgaagacagg cagattcaga actggccatt agctgagttg cgccctgcca gtctttttgc
      241 ccggcacaac gtatggcttc catcgcgtca gaacgtgcct gccccgcgtt gaccagtaac
      301 tccataacaa gttcttcatc tgcaaacatc acaatctcct tcctgggtaa atggaattat
      361 ccgttagttg tcatgttatt gactttgttg gcggcaataa caaaaggtaa atagatcatt
      421 gtcgccacac ctaaacataa aaaaccaacc agtaaagcca gccagttccc cccggttccg
      481 aggaaagcaa ttaaaggtcc gggcgtagtc cagggcacgg cataagcaac cggtggaata
      541 atttccatcg aaacaaagaa gtaaccaatg gcacagttaa caatgggaac cataataaac
      601 ggcaccatca tgatggggtt aagtacaata ggaaggtcaa acattatcgg ttcattgata
      661 ttgaaaatac cggggataaa tgacattttt gccagatcac ggtattccgc acgcttagaa
      721 gcgataaaaa tagccaacaa taaccccaac gtcatacctg aaccgccgca gttggcgaat
      781 gcatcattaa tagaagtcca ggtaatcgga tatggcgcgc cccaggtagt gccatgttga
      841 gcggcccagg agagattctc cagattagcc tcagaaaaaa cggtttctcg aattgccgat
      901 gtggtattag gtccgtggat ccccagcacc cacaggaaat tacccacagc ccccaggata
      961 attaccgcaa agatattcgt tcccatatgt ttgagcggcg tctggattaa tgtgtaaata
     1021 agatcgttta gtcctgccgg ggatatcagt gtcaggcaat aattaagtgc ggaaaagaac
     1081 accatgacaa aaaatattgg caataaaact ttaaatgaac gcgctaccgc aggcggtaca
     1141 gctgccggca tcgttatggt gatttttggg tttcgagcta agcgacaaaa tatttcactg
     1201 gtgatcaatg caacgataac agcaacaaac aacccctgcg cgccgagata tttggtcgtc
     1261 aggaatgctt gcccatctat catctgatat ggcgtataaa caataaaaaa tgctcctatt
     1321 gccgttaatc cgcataaaag atcatcttgc tcatagctaa tcgccatatt cctggcgacc
     1381 aggaaagcaa tcataattga catgatattt acagaaccat tcattaccgg agtaaaaata
     1441 gcttgtgctt tttcaaggtt ggggaaaatg ctattcagat gcagtaatcc ggcaataaaa
     1501 ccgtctggcg ataatatggc aaagttaa
//
LOCUS       DAZGJW010000421         1514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZGJW010000421 DAZGJW010000000
VERSION     DAZGJW010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.425"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1431
                     /locus_tag="ACHKIK_005504"
     CDS             <1..1431
                     /locus_tag="ACHKIK_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO9086423.1"
                     /translation="HPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRT
                     DDERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMS
                     LSRKPEMTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLA
                     EKLQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQV
                     EPEYTPARKVHFYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRL
                     PGQQHDEESGLYYNRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYN
                     LYQLLYDVWHDDSYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATAC
                     GHAGIGGGINAAITYSETKSLPASGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTE
                     SVGIGAGVDASVMTLACRTWQECWVN"
BASE COUNT          378 a          338 c          473 g          325 t
ORIGIN      
        1 cacccggaca gtaccctcac ggcgtggccg gataaccgca tcgcagagga tgcgcactat
       61 gtttaccact atgatgaata cggcagactg acggagaaga cggaccttat cccggcgggt
      121 gttatccgga cggacgacga gcggacccac cactaccact acgacagcca gcaccgtctg
      181 gtgttctaca cgcggataca gcatggcgag ccactggtcg agagccgcta cctctacgac
      241 ccgctgggac ggcgaatggc gaaacgggta tggcggcggg agcgtgacct gacggggtgg
      301 atgtcgctgt cgcgtaaacc ggagatgacg tggtacgggt gggacggcga ccggctgacg
      361 acggtacaga ccgacaccac gcgtatccag acggtatatc agccggggag cttcgcgccg
      421 ctcatccgca tcgaaacaga caacggcgag cgggagaaag cgcagcgccg cagcctggcg
      481 gagaagctgc agcaggaagg gagcgaggac gggcacggcg tggtatttcc ggctgaactg
      541 gtgcggctgc tggacaggct ggaggaagaa atccgggcag accgcgtgag cagtgaaagc
      601 cgggcgtggc ttgcgcagtg cgggctgacg gtggagcaac tggccagaca ggtggagccg
      661 gaatacacac cggcgcgaaa agttcatttt taccactgcg accaccgggg cctgccgctg
      721 gcgctcatca gcgaagacgg caatacggcg tggcgcgggg agtatgatga atggggcaac
      781 cagcttaatg aagagaaccc gtattacctg caccagccat accgtctgcc ggggcagcag
      841 catgatgagg aatcagggct gtactataac cggaaccggt actatgaccc gctacagggg
      901 aggtatatta cacaagaccc cattgggctg gcggggggat ggaacctgta taattaccca
      961 ctgaatccga taataaggat ggatcctttg ggtttgtata atttatatca attattatat
     1021 gatgtttggc atgatgattc atatggaaca tcatcaattg atattactgg cagtggagat
     1081 ctaatatcat taggtggtca tgcaggactt ggcgttgcgt ttgctaaaaa gaaaggtgaa
     1141 atgttatctg atatttgtat ttatgctaca gcatgcggac atgcaggaat tggtggtggg
     1201 ataaatgcgg ctatcacata ttctgagacc aagtctttac ctgcatcggg agtcagcaat
     1261 tcagtaggtg taacggttgg cggcggagtt ggggggcatt ttgcgtatac ttatgtagtg
     1321 gatgttgata atccagaatc atcgacagaa tctgttggta tcggtgcagg tgttgacgct
     1381 tcagttatga ctctggcttg tagaacgtgg caagaatgct gggtcaatta acaggaggaa
     1441 atatgaaaaa atatatattt ttagttttat ttttattgtg tggttgttct ccagtggaca
     1501 tagagccagc taga
//
LOCUS       DAZGJW010000422         1513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZGJW010000422 DAZGJW010000000
VERSION     DAZGJW010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.426"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1513
                     /locus_tag="ACHKIK_005505"
     CDS             <1..>1513
                     /locus_tag="ACHKIK_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO9086424.1"
                     /translation="SDLAEKLTHKLKEGWQPFGSPVAITPYTLMQAIAAEGDVTTPVL
                     VKPSDGEGTVISATRDTEYYFVVVLAGQSNSMAYGEGLPLPETYDRPDPRIKQLARRS
                     TVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPF
                     IPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLYKDLIGRTKAAL
                     KKNPKNVLFAVLWMQGEFDFGGTPVNHAAQFGALVDKFRADLADMAGQCVGGSAGGVP
                     WICGDTTYFWKQKNESSYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDI
                     PGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAILRHAGRVALNAGASS
                     TVSEVRPSSPSGAEATGVTALLSYLASESEGSLKVQGWSASGGRAEVVSDAEGTGGKA
                     VKLTKEAGKSSWVLEYAAGNGAALLQKGGQIRCRFKVSGALAANQYVMAFYWPV"
BASE COUNT          358 a          365 c          490 g          300 t
ORIGIN      
        1 cgtcagacct tgcggaaaag ctgacacaca aactgaaaga gggctggcag ccgtttggta
       61 gtccggtggc catcacgcct tatactctga tgcaggccat tgcggcggaa ggtgatgtca
      121 ccacacctgt gttggtgaag ccgtcggatg gagaaggcac agtaatcagc gccaccagag
      181 acacggagta ttactttgtt gtggttctgg cggggcagtc aaacagcatg gcatatggtg
      241 aaggccttcc gctgccggag acatatgacc gtccggaccc gcgtattaag cagctggcgc
      301 gccgcagtac ggtgacaccg ggcggtgcag catgcaaata taacgacatc attccggcgg
      361 accattgtct gcatgatgtg caggacatga gccgccttaa ccatccgaaa gcggacctgt
      421 caaaggggca gtacggaacc gtggggcaag ggctgcatat cgccaaaaaa ttgctgccgt
      481 ttataccggc gaatgcgggc attctgctgg ttccgtgctg tcgtggtggt tcagcgttca
      541 ccaccggagc cgatggcaca tacagtgacg cgagtggtgc ctcggagaat tcaacccgct
      601 ggggtgtgga caagccgctg tataaggacc ttatcggtcg aacaaaagca gcactgaaga
      661 agaacccgaa aaatgtgctg tttgccgtgt tgtggatgca gggggaattt gattttggcg
      721 gtacgccggt aaatcacgca gcacagtttg gtgcgctggt tgataaattc cgtgcagacc
      781 tggcggatat ggcaggtcag tgcgtcggtg gctctgctgg cggtgttccc tggatatgtg
      841 gagatacgac gtatttctgg aagcagaaga acgaatcctc gtaccagacg gtgtacggca
      901 gctacaaaaa caaaacggaa aagaatatcc atttcgtacc gttcatgacg gatgagaacg
      961 gggtgaatgt gccgacgaac aaaccggaag aagacccgga cattccgggt atcggatatt
     1021 acggttcgaa atggcgtgac agctcagcca cctggacgtc acaggacagg gcgagccatt
     1081 tcagttcatg ggctcgccgc gggattattt ccgaccgtct ggcaacggcg attttgcgcc
     1141 atgcgggaag agtggcgcta aacgcggggg catcatcgac agtatcagag gtgcgcccgt
     1201 catcgccttc cggtgcagaa gccacaggcg tcacagcact gctctcttac cttgccagcg
     1261 agtcagaggg aagcctgaaa gtacagggat ggtcagccag tggcggcagg gcagaagtgg
     1321 tcagcgatgc ggagggaacc ggaggtaagg cagtgaagct gaccaaggaa gccggtaaaa
     1381 gcagctgggt gctggagtac gccgcgggca acggtgcggc tctgttacag aaaggggggc
     1441 agattcgctg ccgctttaag gtttcgggag cgctggctgc gaaccagtat gttatggcgt
     1501 tttactggcc ggt
//
LOCUS       DAZGJW010000423         1510 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZGJW010000423 DAZGJW010000000
VERSION     DAZGJW010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.427"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(97..186)
                     /locus_tag="ACHKIK_005506"
     CDS             complement(97..186)
                     /locus_tag="ACHKIK_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086425.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLAVIALLII"
     gene            complement(241..453)
                     /gene="cspF"
                     /locus_tag="ACHKIK_005507"
     CDS             complement(241..453)
                     /gene="cspF"
                     /locus_tag="ACHKIK_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HFO9086426.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            754..969
                     /gene="cspB"
                     /locus_tag="ACHKIK_005508"
     CDS             754..969
                     /gene="cspB"
                     /locus_tag="ACHKIK_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HFO9086427.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIERGAKGPAAANVIITD"
     gene            complement(1333..1503)
                     /gene="ynfU"
                     /locus_tag="ACHKIK_005509"
     CDS             complement(1333..1503)
                     /gene="ynfU"
                     /locus_tag="ACHKIK_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein YnfU"
                     /protein_id="HFO9086428.1"
                     /translation="MSERKDSKSRRNYLVKCSCPNCTQESEHSFSRVQKGALLICPHC
                     NKVFQTNLKAVA"
BASE COUNT          463 a          264 c          318 g          465 t
ORIGIN      
        1 tgttaactta ttatttagct ggtgacttgg ttatttgcct gatgtttaaa atgttttctt
       61 ccagtacaat gtccctaaac acaatgagtc tgcttattat attattagca gagctattac
      121 ggccaaagta cagcataagc ttttaaagcc aatcaaccag tcatcaagac agacggggtt
      181 attcataaaa actctccatg tgtgatccga tggggcctga aattaaagct ttaatatagc
      241 tcatgaaagg taaacattgg cagctgaagg gccacgcaga ccatttatcc ggcaaaattc
      301 cacgcgtaat ccggtggtaa tttcttctgc atcgcggaga ttgagcgctg aaacatgaag
      361 ctggacatcg atacgaccat cggatggggt gataagaccc ttgccgcttt tgccgtcaaa
      421 ggttttgaca attcctgtca ttttacggga caaaaaaatt ccttaatact gataacttgg
      481 cgcactatac acacgttcct gaagaaagct atagtttttt gatggggttg aagatggctg
      541 gatgtctaaa ataaacattg cttcatatgt tcaactatgc gttaatgatt gcgtcggttt
      601 gaagaacaga cgatatacga agtagtttac taaagcagtt ctcatttcag gtgttattca
      661 cttattcctt ctttgagtct ctccaattaa gtacgaagtc gtttctgtta tgcaaaccat
      721 ttatgccgaa aggctcaagt taaggaatgt agaatgtcaa ataaaatgac tggtttagta
      781 aaatggttta acgctgataa aggtttcggc tttatttctc ctgttgatgg tagtaaagat
      841 gtgtttgtgc atttttctgc gattcagaat gataattatc gaaccttatt tgaaggtcaa
      901 aaggttacct tctctataga gagaggtgct aaaggtcctg cagcagcaaa tgtcatcatt
      961 actgattaaa attcatcgct cgtctgtata cgataacgaa gaaggctgat gcctgagtag
     1021 agatacggac agagtagtga atattggatc tctttaataa aaagtaagga ggtccaatac
     1081 atgaaacaat ggctagcata tttggcaaaa tcttaatcag gaaaagtatg ctaaccattg
     1141 tggtgaagtg caggtttgct gcatgaatag tttcacagca gaagctaact gctggcatcg
     1201 caaaacaaag tgcgtaagtg gatgactccc acaaaaagca ccacaatctc aaacccgctc
     1261 aggcgggttt tttattatct gctttaaata tattattaaa atataaaaaa tacttgttac
     1321 taataaaatc aatcaggcta cagctttaag atttgtctgg aatactttgt tgcaatgagg
     1381 gcagatcaaa agggcacctt tttgtactct tgaaaaactg tgttctgact cttgggtgca
     1441 gtttgggcag gaacatttaa cgagataatt acggcgtgat tttgagtctt tacgttctga
     1501 cataggcttt
//
LOCUS       DAZGJW010000424         1507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZGJW010000424 DAZGJW010000000
VERSION     DAZGJW010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.428"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..225
                     /gene="ydcD"
                     /locus_tag="ACHKIK_005510"
     CDS             <1..225
                     /gene="ydcD"
                     /locus_tag="ACHKIK_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdcD"
                     /protein_id="HFO9086429.1"
                     /translation="INLNFFNDILYSVRLKNISKLENMEFCATKQRVYFSDKNKKASY
                     KIINYGDYYDVDYYDNNLKNEVFDWIGKWS"
     gene            complement(140..325)
                     /locus_tag="ACHKIK_005511"
     CDS             complement(140..325)
                     /locus_tag="ACHKIK_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086430.1"
                     /translation="MIYITIFMILPCPVPCSHVFLYVFYIFLFLVLFIMTIYQSSQKL
                     HFSNCYHNNQHRNSLHN"
     gene            407..1507
                     /locus_tag="ACHKIK_005512"
                     /pseudo
     CDS             407..1507
                     /locus_tag="ACHKIK_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
BASE COUNT          557 a          200 c          346 g          404 t
ORIGIN      
        1 ataaatctta attttttcaa tgatattctt tatagcgtgc gattaaaaaa cattagtaaa
       61 ctggagaata tggagttctg cgctacaaaa caacgggtgt attttagtga taaaaacaag
      121 aaggctagtt ataaaataat taattatgga gactattacg atgttgatta ttatgataac
      181 aatttgaaaa atgaagtttt tgactggatt ggtaaatggt cataatgaac aacaccagaa
      241 agagaaaaat ataaaataca tacagaaaga catgagagca agggacgggg cagggcaaga
      301 tcatgaaaat tgtgatgtaa atcacgattt tcatcttggc tttaacgcct acaggtgatc
      361 aaaatttaac ctttggaata actaaaaaga taaaaaagga cgccaggtga gtattcaaag
      421 tttgcttgat tatatttcag tgatccctga tatacgacaa caaggaaagg ttaaacataa
      481 attatctgct attttgtttc tcaccgtatg tgcagtaatt gcaggtgccg atgagtggca
      541 ggaaattgaa gattttggac atgaaagact tgaatggcta aagaaatatg gtgattttga
      601 taatggcatt ccggtcgatg acaccattgc acgcgttgtg agtaacattg acagtttggc
      661 ctttgaaaag atgtttattg aatggatgca ggagtgccat gaaatcactg atggtgaaat
      721 tatagcaata gatggaaaga ccataagagg ctcctttgat aagggaaaaa gaaaaggagc
      781 aatccatatg gtgagtgcat tctcgaacga aaatggtgtt gtactggggc aggtgaaaac
      841 ggaagccaaa agtaatgaga ttacagccat tccagagttg cttaacctac tgtatttaaa
      901 gaaaaatttg ataaccattg atgctatggg ctgtcagaaa gatatcgctt cgaagatcaa
      961 agataaaaaa gcagattatc ttctggcagt aaaaggcaat caggggaaat tacatcatgc
     1021 attcgaggaa aaatttcctg taaatgtgtt ttctaattat aaaggcgatt cgtttagtac
     1081 gcaggagata agtcatggaa gaaaagaaac acgtttgcat attgtcagta acgtaacgcc
     1141 tgaacttttg tgattttgaa ttcgaatgga agggattaaa aaagctttgt gtagcattgt
     1201 cattcaggca gaagaaagaa gataaatcag cagaaggtgt aagcatccga tattatattt
     1261 catcaaagga tatggatgct aaagaatttg cacatgctat cagagcgcac tggctgatcg
     1321 agcacagtct tcattgggtg ttagatgtaa aaatgaatga agatgccagc cggataagaa
     1381 gaggaaacgc agcctaaata atatctggaa taaagaagat ggcactgaat ttattaaggg
     1441 attgcaaaga cattaagggt gaagaggaaa agaaagaagg ctgcgttaaa cacagggaaa
     1501 gatcatc
//
LOCUS       DAZGJW010000425         1501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZGJW010000425 DAZGJW010000000
VERSION     DAZGJW010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.429"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..947
                     /locus_tag="ACHKIK_005513"
                     /pseudo
     CDS             1..947
                     /locus_tag="ACHKIK_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004019902.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1358..>1501
                     /locus_tag="ACHKIK_005514"
     CDS             1358..>1501
                     /locus_tag="ACHKIK_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO9086431.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTAF"
BASE COUNT          486 a          260 c          317 g          438 t
ORIGIN      
        1 atgctttaga aaaaacgacc tttactctgg aaggattaca ggaagcaatc aaaaaatata
       61 gatattattg ctcctgattg tctggtgatt tctgactggg aagatagcca tcggcgaatt
      121 gatttattgg caatcgataa acaggcaaat atagtggtta tcgaattaaa acgtgatgaa
      181 acggggacgc atatggagtt acaggcacta cgatatgcgg caatgattta cacaatgagt
      241 tttgcgaaag catgtgagta ttttcagacg tatttgaaaa aacaaaaccg cgatgccgat
      301 gcgaaagaaa aaatgcgaaa gaaaaaatac ttgaatttgt tgagctggat gaaactgagc
      361 ttgttgattt tggtaaagat atcagaatcg ttttggcttc atcggatttt agcaaggaac
      421 tgactaccac tgcgatttgg ttaagagata aaggggtaga cattcgatgt gttcgtctga
      481 cgccttatag atttaatgat gatgttctga tcaatgcaga gcaaatcata ccggttcctg
      541 aattggaaga atatcaagtt aagtttcgtg aaaaacgtga tgagcagcta attagcagtc
      601 agaaaaaaga gaagggctat acgtggtata tatgcaaaga taaaaaactt aataaacgta
      661 agttagcgct tgagttatta cgcgattgta taaaacaatt taaccctgca agttataatg
      721 atttaataaa tggattaagt gaagacttta aaaaacgtac ggttatactt atcgatcaga
      781 taccagagaa gcaaaagagt cgctatcata tcaacgaaga tgcattgatt acacttcctt
      841 ctggtgaaat tgtcgccatt tctaatcaat ggggtattgt taatattgaa ctcttgattg
      901 tgtttgtttg gcaaaatggt tttgttgttg aaaaagctga acaataaatt tttatattgc
      961 tataaatgtc tacattgaaa aatagggaga gcatttgtag cttttaacat ctcaatgtaa
     1021 gagatggcaa gattatgggg gcgcggtatt atcaacctcc ccccctaaaa acctctttcc
     1081 gatgccactc tacaaccccc taaaaaaccg gtggcgattc acattccacc atgcattccg
     1141 attaacgccg catagccagt tgaactttgc tactctgtga gaggtagtac cttctatcca
     1201 gtgcgaattt aattaatgga ataaatgatt atgagtgaaa gtgatacaat cccaaagaag
     1261 tctacaagtc agattaacaa agcggtattc tttacatctg ctggtaatgc tgccaactta
     1321 ctgatttagt gtatgatggt gattttaagg tgcttgcgtg gcttccattt ccatcagatg
     1381 tccttcctgc tccgctactg aaggcgtggt gcgtaacggc aaaagcactg ccggacatca
     1441 gcgctatctc tgctctcatt gccgtaaaac atggcaacta cagttcactt acaccgcctt
     1501 t
//
LOCUS       DAZGJW010000426         1500 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZGJW010000426 DAZGJW010000000
VERSION     DAZGJW010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.430"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..756
                     /locus_tag="ACHKIK_005515"
     CDS             <1..756
                     /locus_tag="ACHKIK_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease"
                     /protein_id="HFO9086432.1"
                     /translation="NKIVLSLRGNHSGSVKRIEFQGNNGGNINDFLGRIVMYTLDGIE
                     LELSANKESPVVAAKIDIPKFEKYHGDIGILDPLNILENNNLYFVKENISEFDFGNYR
                     VVSSQDTFIYKVTNPGAQFYISPGKPSHIFGEGGCHYYFSGVKQPSHLIFLNNDNRNP
                     ETINVSSFTETSEEVKIFANAKKNKCTLKFIWPYGSFELILRPRNSLRIDLNTSETKI
                     SLSHGTMLLRDIFELSQRSSGDVLIYNTLWQYH"
     gene            921..1217
                     /locus_tag="ACHKIK_005516"
                     /pseudo
     CDS             921..1217
                     /locus_tag="ACHKIK_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          466 a          287 c          322 g          425 t
ORIGIN      
        1 aataaaatag ttttatcact aagagggaat catagtggtt ctgtgaaacg cattgaattt
       61 caagggaata atggtggcaa tataaatgat tttttaggga ggatagtaat gtatactctt
      121 gatggtatag agttagaact atccgctaat aaagaaagcc ccgtagttgc agcaaagatt
      181 gatataccaa aattcgaaaa atatcatggt gatattggca tattggatcc tcttaatatt
      241 ttagaaaata ataatcttta tttcgtcaaa gaaaatatca gtgaatttga ttttggtaat
      301 tatagagttg tttcttccca agacacgttt atatacaaag taacaaatcc gggggcgcag
      361 ttttatatat caccaggaaa accatcacat atttttggag agggaggatg tcactactat
      421 tttagtggag taaagcaacc gtctcatctt attttcttaa ataatgataa cagaaatcca
      481 gagactatta atgtatcaag tttcacagag acatctgagg aagtaaaaat atttgctaat
      541 gcgaaaaaaa ataaatgcac tctaaaattt atatggccct atggttcttt cgagttgata
      601 ttaagaccaa gaaatagctt acgcatagat ttaaatacat cggaaacaaa aatatcttta
      661 agtcatggca ctatgttatt aagagatata ttcgagttat ctcagcgatc tagtggggat
      721 gttctaatct ataatactct ctggcaatat cattaagcct acgttcttat tcattgagca
      781 ccgaacgtta ttgaagcgga gtatcgaggc tgaccgccta aataactgct cggaatagta
      841 gatcactcag ggggaactca gcctggttta tgcgatctga tcaatcgctg aatatcccaa
      901 atcaccagcc ggactgagca atgccgatca tagcaccaat acccagaatc gaacgacgtc
      961 tgatgcagaa aactatccat aaaacaaagg acaaaaacca cgcccggcga ctcaccgcca
     1021 tgctgatgct gcatcgtggt gacaccgtct gtcatgttgc cagaacgctc tgctgcgccc
     1081 gctcatccat tggtcgctgg attaactggt tcacgcagtc tagcgtagaa ggtctgaaat
     1141 cattaccgtt aggacgtggg agacgctggc cgttcgaaca tatttgcgca ttgctgcgcg
     1201 agcttgttaa gcattctcgg ggtctgaggg ccaatggaac gaaaacgtac gttaagagca
     1261 taatttgttg tttgtattga atttagtaat aaagcccgtg ggggcttttt tatgctctca
     1321 ccataagagt ggcggtgatt attaaccata aaataaaacg cctacatctt gaaaattctg
     1381 acacccgtta ctttattcgt accccttata atggggtgtt agccagccag agccggcatg
     1441 attactgccc ccagtcgtcc atgatccggg ggggatgtca ccgggtctgg tggggcgctg
//
LOCUS       DAZGJW010000427         1491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZGJW010000427 DAZGJW010000000
VERSION     DAZGJW010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.431"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..174)
                     /locus_tag="ACHKIK_005517"
     CDS             complement(<1..174)
                     /locus_tag="ACHKIK_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO9086433.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAITAEGDVVVSGA"
     gene            434..769
                     /locus_tag="ACHKIK_005518"
     CDS             434..769
                     /locus_tag="ACHKIK_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO9086434.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNIVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQNP"
     gene            840..956
                     /locus_tag="ACHKIK_005519"
                     /pseudo
     CDS             840..956
                     /locus_tag="ACHKIK_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1050..1181)
                     /locus_tag="ACHKIK_005520"
     CDS             complement(1050..1181)
                     /locus_tag="ACHKIK_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO9086435.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1217..1489)
                     /locus_tag="ACHKIK_005521"
     CDS             complement(1217..1489)
                     /locus_tag="ACHKIK_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086436.1"
                     /translation="MDEASGCFILRQRIDIVNAARAKAFSRLTVLFCTPDRLLGRDVI
                     ILNSDAIQRVCDEFMVANSELFALVQEYNRIARTCGMDELRITHLG"
BASE COUNT          497 a          372 c          246 g          376 t
ORIGIN      
        1 tgcaccactg accaccacat caccttctgc tgtaatcgcc tgcatcagag tataaggggt
       61 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      121 cgcaagatct gatggcgacg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      181 ttccaccatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      241 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      301 gagatataaa ccaccctgta tttaaataaa caataaacaa catcagaaaa ataattctgc
      361 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      421 acaaggaaag taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      481 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
      541 cttacctcct gaaagtatat ttacactgac tcacacaggt atcatgctca ataacaaagt
      601 gctacctgta accatttaca acatagtacc attcaataaa acattctgga atttaatcaa
      661 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
      721 ttgcactctg caaatatgtc cttatgggct aaaacaacaa aatccataag gagtttactc
      781 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
      841 tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
      901 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
      961 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1021 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1081 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccagcac tgacattatc
     1141 cggctgctgc aatgccattc acaaaaacag taagcaatca ctttttaccg taacaggtga
     1201 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1261 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1321 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccaaaagac
     1381 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1441 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata t
//
LOCUS       DAZGJW010000428         1484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZGJW010000428 DAZGJW010000000
VERSION     DAZGJW010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.432"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..70)
                     /locus_tag="ACHKIK_005522"
     CDS             complement(<1..70)
                     /locus_tag="ACHKIK_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO9086437.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAW"
     gene            complement(141..>1484)
                     /locus_tag="ACHKIK_005523"
     CDS             complement(141..>1484)
                     /locus_tag="ACHKIK_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9086438.1"
                     /translation="RIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQVETT
                     ARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSDDASGYLDFFKG
                     EIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSNEITQTVNKKLEDQSAAIQQIQK
                     VQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPDGMQSQVLLAADRIAMINPA
                     NGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSLTPDGRLTAKNADISGSV
                     NANSGALNNVTINQNCTIKGMLEATQVRGDFVKAVSKAFPKKVGTWGNTETPNGTVTV
                     TISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETK
                     GAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
BASE COUNT          344 a          399 c          332 g          409 t
ORIGIN      
        1 cccatgcagg cgcaccggat acggccagac agatggctgc ggacaaaatg gcggcataaa
       61 gtttacgcat aattacctct cgcttttctg caataaaaaa ggcgtcattc ctgacgccct
      121 ttattggggt tataaatatt tcaacgaata ctgatgccgg aagcagcttt tttggtcaca
      181 atcaccgtac agtcggtgat attgcctgcc ccctgattgc ctttctggaa aatcttaaac
      241 tccagagtga cgctaccacc accactaggc atatcaataa ctgcactgta actaccggga
      301 atggcccctt tagtttctct ggatgcgatt aatacgccgt ttttgcgaac ttcaaaacca
      361 taccccgtgt atcgcgtgcc tcctgggtta tttccgctcc ctggatcgtc atacgctata
      421 ccgttaaaaa taatgggcgg aataataatc tggcggtcaa agttatgatc atcgctgatg
      481 gtgactgtaa ccgtaccgtt tggtgtttcc gtgttacccc acgtaccgac ttttttcggg
      541 aaggcttttg atacagcttt aacgaaatcc cctctgacct gggtcgcctc cagcatgccc
      601 ttaatcgtac agttctggtt aatcgtgaca ttgttgagcg ctcctgagtt cgcattcaca
      661 ctgccactga tatccgcatt tttcgccgtc agtcgcccgt ctggtgtcag ggaaaatgcc
      721 ggaggattac cgccgctggt aatggtggga gccgtcagat atttcaggaa cacttcattc
      781 atgaatatct gatcgccctg accaacaaac atcggctttg tgttgccatt cgcaggatta
      841 atcatcgcaa tcctgtctgc tgccagcagc acctgactct gcatgccgtc gggggtgttc
      901 tcaataccgg caccgatacc cgcaatataa aggcgtccgt cctgcatctg ctgcagcttc
      961 actgcccaca tgctgttcag gttattattt gtatcaacct gaaccttctg tatctgctgg
     1021 atcgctgcac tctggtcttc cagtttctta ttgacggtct gtgttatttc attgctgaca
     1081 tccgttatgg acgtcctgat ttcagtcagg tcaggcgcaa gctgaccgtt atcaatctgc
     1141 gtccacagct cctgagccag atgggttttc cctatctcgc ctttgaaaaa atccagatag
     1201 cctgatgcat catcactcgg ctgaccgaca gcctctacaa atgccgattt gccaacggtg
     1261 ttcacactgc ggatataaaa gtaataatca tggcccggtt tgatattgat actggcagct
     1321 atccagtaca gcgccgtgcc aagatagcgg gctgtggttt caacctgcct gatatcagca
     1381 atccgctttt ccgagaacca gaactcaaac tgtaccgtcg gatcataaac cgcaagatgc
     1441 ggcgtggcgg ttatctgaaa atagcccggt gtcagctcaa tccg
//
LOCUS       DAZGJW010000429         1476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZGJW010000429 DAZGJW010000000
VERSION     DAZGJW010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.433"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>1476)
                     /locus_tag="ACHKIK_005524"
     CDS             complement(<1..>1476)
                     /locus_tag="ACHKIK_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9086439.1"
                     /translation="SFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLC
                     RRPNAQQNRIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGE
                     VFYRITPDRNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYVAGLAATQGHHIQEN
                     STSFFRNGGRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTT
                     FSPVDAQTVEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIE
                     SIELLLDEALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLP
                     PLAGGDALYLQQQNYSLEALSRRDAREDPFASAGKTVSSQLPDGASDGNKAISETEHD
                     AVKAMFRGDTEKMTERELSIIRALGEEFSTVLADLQRTFEGKMASQAQAFEEKLTSLS
                     AVLQKHVTVDEVRPVLQAMVDDAVGAIPVPRDGRDYDPDVLQ"
BASE COUNT          309 a          468 c          352 g          347 t
ORIGIN      
        1 ctgcagtaca tccggatcat aatcacgacc atcacgcggt accggaatgg cccccacagc
       61 gtcatccacc atcgcctgca gaaccggacg cacctcatcc accgtcacat gcttctgtaa
      121 taccgccgac agggaagtca gtttctcttc aaacgcttgt gcctgcgagg ccatcttccc
      181 ctcaaatgtg cgctgtaaat ccgccagcac tgtggagaat tcttctccca gtgcacgaat
      241 aatggacagt tcccgttccg tcattttctc agtatccccc ctgaacatcg ctttcaccgc
      301 atcatgctct gtttcactga ttgccttatt accgtcagat gcgccgtcag gcagctgtga
      361 tgaaactgtt ttcccggcag acgcgaacgg atcctcacgg gcatcacgac gggacagcgc
      421 ctccagactg tagttctgct gctgaagata cagtgcatca ccgccggcca ggggcggcag
      481 gttctcccgt ttacgggcct cattgggcgt gagaagcgta tttttcaccg catcccccag
      541 cgttttcatg cgccgctcac tgtccattct cagcagcgtg gtgacatcaa attctgtact
      601 ctcgttttcc cccgtttcca gcgcctcatc cagtaacagt tcaatggact caatcagcgt
      661 ctgcaggcac tgggaataat actgctgctc cagcgcctcc acgttgtcac tggaaggcgg
      721 ttgtcccacg ccaatcttgt aggccgggac acggaacacc gaacagacaa tttcagcggt
      781 catcttcagt tgttccaccg tctgcgcatc cacaggtgaa aacgtcgtgg ggttgtattt
      841 tgccccgttg ctcagaatgg ccgttttccc cgcattttcg cctgtatacc cgctgtccca
      901 gttgctcttc agttttttcg cattttcttc cgtaatactg ccggggatct caatcacccc
      961 ggacggcctg ccgccatttc tgaaaaaaga cgtcgaattt tcctgaatat gatgcccctg
     1021 cgtggccgcc agcccggcga catacaccgg cggcaatcct ataagcggat gaaaaaaaca
     1081 gttaaaccgg tcgtggatca cttcccgggc aggcaccgtc accgcctccg tgatcccgca
     1141 gttccggtcc ggcgtgatgc ggtagaacac ctcgccgtca tccgccacca gaggttcaac
     1201 ccggctccag tccagaatac gcagttcttt gatctgcccc cgggcattac ggattttcag
     1261 caccaccgta ttgccatgac gcagtttggc gttcagccac agttcaaaaa actggatgcg
     1321 gttctgctgg gcgttgggac gacgacagag gcgggcaata tccccccggc gcgtttccct
     1381 gcgtatccca tgcgcatccg tctgcataag acgcagccgc attttggcga tatcctggga
     1441 tatcagcgaa atacatgcaa acaccgcatg aaagga
//
LOCUS       DAZGJW010000430         1469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZGJW010000430 DAZGJW010000000
VERSION     DAZGJW010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.434"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..197)
                     /locus_tag="ACHKIK_005525"
     CDS             complement(<1..197)
                     /locus_tag="ACHKIK_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086440.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTV"
     gene            complement(261..>1469)
                     /locus_tag="ACHKIK_005526"
     CDS             complement(261..>1469)
                     /locus_tag="ACHKIK_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO9086441.1"
                     /translation="HIRTITHRVTDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQY
                     KAEDMLREAIGGLASRPEGFVVYTTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLP
                     VIFEHPPEMVESGANLLMENLAMVNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSK
                     HANVEIGLALRSDRWAGADFWEEQGRCISLDDILRRADVVTVGIDGGGLDDLLGMYVT
                     GRDRETREWLGWGHAWAHETAVVRRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYV
                     RRIHEAELLDHIGIDPSGVGQILDSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAE
                     GVLVHGDQPLMAWCVGNARVEPKGNAILITKQASGRGKIDPLMALFNAVSLMSLNPEP
                     KKKEYAVFFI"
BASE COUNT          319 a          485 c          359 g          306 t
ORIGIN      
        1 accgtacact gccccaccgg gcgggaatgg tcatgctgcc agagaaacgg tatcgcactg
       61 ccaaactccg cgccctccgg ctccaggatg tcaccatccc gatccggaga aggcgttgac
      121 gcaatcccgg tgatcacccg ttcatcctca ctgaaggatt tcaccgtcag cagggaacag
      181 gcccgtttaa gagtcacatc agcctcctga aaataaaaaa accgccggag cggttcgtga
      241 tggttacagt gtgaacaggg ttatatgaaa aaaaccgcat attctttctt tttcggttcc
      301 gggttaaggg acatcaggga gaccgcattg aacagcgcca tcagcgggtc aatttttccc
      361 cgtccactgg cctgtttggt gataagaatg gcgttacctt taggctccac ccgggcattg
      421 ccgacacacc aggccatcag gggctggtca ccatgcacca gcaccccttc agccagtttg
      481 cgctcggtgg ttttaatggc cccgcccagt ttccagccct ggcttatccc caccacaatt
      541 ccgtcgggga tcccggcttc cgccagtgaa tccagaatct gccccacccc tgacgggtca
      601 ataccgatat ggtccagtaa ctcagcctca tgaatgcgac gcacatattc cgccacttcc
      661 gccgtgtcat ccccgacacg ccggacaatg gtcatatctc cacaggcaac aagatcctga
      721 aaccgggacg cctcgctctt ccgtcggacc accgcggttt catgcgccca ggcatggccc
      781 cagcccagcc attcgcgggt ctcccggtca cgcccagtca catacatccc cagcagatca
      841 tccagccctc cgccgtcaat ccccaccgtc accacatcag cacgacgcag gatatcgtcc
      901 aggctgatac aacggccctg ctcttcccag aaatcagccc ccgcccagcg gtcagagcgc
      961 agggcaagac caatttccac attggcgtgt tttgacatga acccccggaa tgtctcttca
     1021 ccggcttccc gggctttacg gtactcccgg tacagaaagg cctcatccac tgaatagccg
     1081 agattcggat tgaccatggc gaggttttcc atcagcaggt tagccccgct ttccaccatt
     1141 tcaggagggt gttcaaatat caccggcaga aagtgcggat catgaatttt gccgtcacgg
     1201 acatcccggg catactgcag tttctgtctg aacaccccgg cgggcggttc attcgactgg
     1261 gtggtcgtat acaccacaaa cccttccgga cgggaggcaa gcccgccgat ggcttcacgt
     1321 agcatgtctt ccgccttgta ctgcttgcca aacagccaca gttcatcaat cagtgtcccc
     1381 acggacttga taccggacac cgtattcgga tcggctgcca ccaccttcag ggtggtgtcc
     1441 gtcacccgat gggtgatggt ccggatatg
//
LOCUS       DAZGJW010000431         1464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZGJW010000431 DAZGJW010000000
VERSION     DAZGJW010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.435"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..270)
                     /locus_tag="ACHKIK_005527"
     CDS             complement(<1..270)
                     /locus_tag="ACHKIK_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086442.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTS"
     gene            complement(365..571)
                     /locus_tag="ACHKIK_005528"
     CDS             complement(365..571)
                     /locus_tag="ACHKIK_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9086443.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGGFPAPIKM
                     ERSSRWLKSEVEAWLQARIAQSRP"
     gene            1328..1453
                     /locus_tag="ACHKIK_005529"
                     /pseudo
     CDS             1328..1453
                     /locus_tag="ACHKIK_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
BASE COUNT          379 a          340 c          413 g          332 t
ORIGIN      
        1 actggtgcat aaattcacta tcagatcatc atccaggggc ggtgtggccc gtttgtcagg
       61 gccataaatc agccggtgca ggctctgggg tacgatggca atctccggtt tttcaccatt
      121 gaatgacacc caaccgggcg tcagattatg gaagtagtag atattcggat gacacacgcc
      181 ccaggatacg gcgtgccggg tgaagccggt cagccatttc tggtagcttt cgtggagcca
      241 ttcgtagcgg gtctgatact gttgtttcat tttcatattc ctcacgggga tataacaggc
      301 agcagaatgc cgccggcagg ccggagatta cgtctgctgc gggtgaacgg ataacggtca
      361 gaaattacgg acgggactgt gcaatacgcg cctgcagcca ggcttccact tcacttttca
      421 gccagcggga gctgcgctcc attttgatgg gggcaggaaa gcccccatct ttgatgagtt
      481 tgtaaaacca cttatcggtt aagccggtaa gttgagtgat aaacgccatg tcgaccatct
      541 ggtcatccat cagcgaaaca ggggtagcca tgagaatgtt cctccggatg ttgttaaacc
      601 agccggtgtg gtcatgaaaa cagcgcgcca ccttcactgg cataacaacc ggggagaaac
      661 gtaaaaaata atcgggaatg tggctgtccg gagataaacg gggggattgc gatggcagag
      721 ccgtagcgga atcccgggtg gttgttgcga aaagatgctt ccgcacgcac gcgcgataaa
      781 ggagggagga ctttcgtgag acaaggggaa aactagcgtt ctgataattg cagagttgtt
      841 ttcggaggtg gttacagtga gtcagcccgt tcctggcgag gaggagactg atatcaccga
      901 tgggataagg ctgtctgatt ggtattataa atataagaag tcatcagaga gtaacgtaaa
      961 ggatttgcca gagtaagcta agagagtaaa tctctccggt aacagagcgc tgtgaatcgt
     1021 tcatgtatca gagtgcagat acatgaacgc tgatgcgtaa aatcagcagt gacacgccag
     1081 ctccccctgt gcccattcct gtataagaca tgaactgagc gtattaatgc tcctgccctg
     1141 agactgagcc ggtgagtgag gggctgagaa atccctcttg acactgctct aaaccagaaa
     1201 tacttccccg gttgttgcgg tgcgtccgga tccccgataa cggaacacta aggctgtatt
     1261 gcgggacacc tagtaagtgg cagcccgtaa tacgtcagga gacgcgggaa aagtgaattc
     1321 ccacacgcgt tataaactta cccttgcgga gctggaagcc tttaactctg ccgttgacaa
     1381 ccggctggca gaactgacaa tgaacaaact ttacgatcgc gcgccggctt ccgtctggaa
     1441 atatgtcacc tgaatattga agca
//
LOCUS       DAZGJW010000432         1461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZGJW010000432 DAZGJW010000000
VERSION     DAZGJW010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.436"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..234)
                     /locus_tag="ACHKIK_005530"
     CDS             complement(<1..234)
                     /locus_tag="ACHKIK_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO9086444.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLKR
                     GNDGQPPWFEHDQMTVAAKILLRCTWLDPAEKEA"
     gene            420..1388
                     /locus_tag="ACHKIK_005531"
     CDS             420..1388
                     /locus_tag="ACHKIK_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO9086445.1"
                     /translation="MSQPGSLCVDIDVSKAILDIAASSAIEQFSVGNDSDGFDAIIAE
                     LRMYAIALVLMKATGGLETAIVCALQAEGFELAVVNPRQARDFARAMGYLAKTDSIDA
                     RVLSQMAEVLNRHPERERFIRALPDTECQVLTAMVVRRRQLITMLVAERNRLHPAHPL
                     NRKSINIIIKALEDELARLDADMNSHIHSHFKELAKRLSSIKGVGTMTAAALLAEVPE
                     QGNLSRREISVLVGMVPVNRDSGTMRGRRTIFGGRAGVRTALYMAALVATRFNPVIKV
                     FYMRLLAAGKPKKVALVTCIRKLLTILNAMLRKNEEWNESYHHVAP"
BASE COUNT          363 a          360 c          382 g          356 t
ORIGIN      
        1 ggcctctttc tctgccgggt caagccaggt gcatcgcagc aggattttcg cagcaacagt
       61 catctggtca tgttcaaacc acggcggttg tccgtcattt ccccgcttaa gtgttaacgg
      121 acaaccacag gacacacagc acaaaacgtc ctgtcggttg tgcttcgcgg cttctttcgc
      181 cgtaacaaaa tggcctttgc tgtcattcgc catatagcag ttcagaattc gcatatctcc
      241 tccttgtatc ttgacaattg gttaaaataa ctgtgattag atttggcaag gtgtatgcag
      301 cccgatccct caaggggttc atagcccgta gctcagcgaa tggcgcatac actgagccac
      361 tcatctgaaa cccgaacagt tgtcagtacg ccataacgca tttgcaagga tgggtaatca
      421 tgagtcagcc aggttcactt tgtgtggata tcgatgtttc gaaagcgata ctggatattg
      481 ccgccagcag cgctattgaa caattttctg tcggcaatga cagtgatggc tttgacgcca
      541 tcattgctga gttgagaatg tacgccattg cgctggtttt aatgaaagcc actggaggac
      601 ttgaaaccgc catagtctgt gcgcttcagg cagaaggttt tgagcttgcc gtggttaacc
      661 ccagacaagc tcgtgacttt gcccgcgcca tgggatacct cgcgaaaact gatagtattg
      721 atgccagagt gctttcccag atggctgagg ttctcaaccg gcatccggag cgggaacgct
      781 ttatcagagc actgccggat actgaatgtc aggttctcac tgcaatggta gttcgtaggc
      841 ggcagttgat tacgatgctg gttgccgaga gaaaccggtt gcaccctgct catccattga
      901 acaggaaaag cattaatatc atcatcaaag cactggaaga cgagcttgcg cgactagatg
      961 ctgacatgaa cagccatatc catagccact tcaaagagct tgctaagcga ctgagcagta
     1021 ttaaaggtgt tggcaccatg acggctgctg cgctgctggc ggaagtaccc gaacagggta
     1081 acctctccag gcgtgaaatc agtgtgcttg tcggtatggt tccagtaaac cgggactcgg
     1141 gcacgatgag agggcgacga accatctttg gcggcagggc tggtgtgcga acagccctgt
     1201 acatggcagc gcttgtcgca acccgcttta acccggtaat aaaggtgttt tacatgcgac
     1261 tgcttgccgc aggaaaaccc aaaaaagtgg cgctggttac ctgtatacgc aagctgctga
     1321 ctatcctgaa cgcaatgctc agaaagaacg aagaatggaa tgaatcgtac catcacgttg
     1381 ctccataatt tttacgttca agacagttgc tgagctgtcc ggggaagact gaaacctccg
     1441 gtctggcagt gtgtgcagta c
//
LOCUS       DAZGJW010000433         1456 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZGJW010000433 DAZGJW010000000
VERSION     DAZGJW010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.437"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..358)
                     /locus_tag="ACHKIK_005532"
     CDS             complement(<1..358)
                     /locus_tag="ACHKIK_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086446.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSTIPFLWQHDHSRPVGQCTVRRVREGLEITAMLVKPEPGMPSQMAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYT"
     gene            complement(422..>1456)
                     /locus_tag="ACHKIK_005533"
     CDS             complement(422..>1456)
                     /locus_tag="ACHKIK_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO9086447.1"
                     /translation="SRPEGFVVYTTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLP
                     VIFEHPPEMVESGAHLLMENLAMVNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSK
                     HANVEIGLALRSDRWAGADFWEQQGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVT
                     GRDRETREWLGWGHAWVHETAVVRRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYV
                     RRIHEAELLDHIGIDPSGVGQILDSLAEAGIPDESVVGISQGWKLGGAIKTTERKLAE
                     GVLIHGDQPLMAWCVGNARVEPKGNAILITKQASGRGKIDPLMALFNAVSLMSLNPEP
                     KKKAYEVFFI"
BASE COUNT          319 a          493 c          354 g          290 t
ORIGIN      
        1 aagtgtattc atggggccgg aagcccacag aaagccccct gaccagcccg gtcttaatgg
       61 cagcccaggc ctcatccagc cgggctgcca tctgggaggg catccccggc tccggcttca
      121 ccagcattgc cgtgatttcc agcccttccc tgacccgacg caccgtacac tggcctacag
      181 ggcgggaatg gtcatgctgc cagagaaacg ggatcgtact gccaaactcc gccccctccg
      241 gctccaggat gtcaccatcc cgatccggag aaggcgttga cgcaatcccg gtgatcaccc
      301 gttcatcctc actgaaggat ttcaccgtca gcagggaaca ggcccgttta agagtcacat
      361 cagcctcctg aaaataaaaa aaccgccgca gcggttcatg atggttacag ggtgagcagg
      421 gttatatgaa aaaaacctca tacgctttct ttttcggttc cggattcagg gacatcaggg
      481 acaccgcatt gaagagcgcc atcagcgggt caatttttcc ccgtccgctg gcctgtttgg
      541 tgataagaat ggcgttacct ttaggctcca cccgggcatt accgacacac caggccatca
      601 ggggctgatc accgtgaatc agcacccctt cagccagttt gcgctcggtg gttttgatgg
      661 ccccgcccag cttccagccc tggcttatcc ccaccacact ctcatcgggg atcccggctt
      721 ccgccagtga atccagaatc tgccccacac ctgacgggtc aataccgata tgatccagta
      781 actcagcctc atgaatacga cgcacatact ccgccacttc cgccgtgtca tccccgaccc
      841 gacggacaat cgtcatgtct ccacaggcca caaaatcctg aaaccgggat gcctcactct
      901 tccgtctgac caccgcggtt tcatgcaccc aggcatggcc ccagcccagc cattcgcggg
      961 tttccctgtc acggccagtc acgtacattc ccagcagatc atccaggccc ccgccgtcaa
     1021 tccccaccgt caccacatca gcgcgctgca ggatatcgtc caggctgacg cgcctgccct
     1081 gctgctccca gaaatccgcg cccgcccagc ggtcagaacg cagggcaaga ccgatttcca
     1141 cattggcgtg ttttgacatg aagccacgaa atgcttcctc accagcctcc cgggctttac
     1201 ggtactcccg gtacagaaag gcctcatcca ccgaataacc gagattcggg ttaaccatgg
     1261 cgaggttttc catcagcagg tgagccccgc tttccaccat ttcaggagga tgctcaaaaa
     1321 tcaccggcag aaagtgcgga tcatgaattt tgccgtcacg gacatcccgg gcatactgca
     1381 gtttctgtct gaacaccccg gcgggcggtt cattcgactg ggtggtcgta tacaccacaa
     1441 acccttccgg acggga
//
LOCUS       DAZGJW010000434         1453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZGJW010000434 DAZGJW010000000
VERSION     DAZGJW010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.438"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..266
                     /locus_tag="ACHKIK_005534"
     CDS             <1..266
                     /locus_tag="ACHKIK_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO9086448.1"
                     /translation="PTAVYADYINNTTDFRPKPERIFICGETLKCEDPSIYIRQKYGH
                     HGVGVDQNQQFDNGVMGLNSTKKVLDKNNKIIYSSGLSCFQSH"
     gene            528..>1453
                     /locus_tag="ACHKIK_005535"
     CDS             528..>1453
                     /locus_tag="ACHKIK_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO9086449.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     DNNYQFIVTINSINYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKF"
BASE COUNT          482 a          241 c          282 g          448 t
ORIGIN      
        1 aacccacagc tgtatatgct gattatatta ataacactac tgatttcaga ccaaaacctg
       61 aacgtatctt catatgtgga gagacactca aatgtgaaga tcccagcata tacattagac
      121 aaaaatatgg ccatcatggg gtcggggtag atcagaatca acaatttgat aatggtgtta
      181 tggggctaaa ttccacgaag aaagtacttg ataaaaataa caaaataatt tattcctcag
      241 ggcttagttg ttttcaatct cattaaaact ccatgaatac ggtggtatgc ctttttaatc
      301 ctgccagtat ttcataggtt ttcttaatcg attgtagagg tatgtactga tgacgcctct
      361 acaatcttta gggactttct ctgctatgag ataagaaatt tcatattatg ctttaaggct
      421 gaatgatttg tgcaaaatat aattattttt ccatcgaaca tgaggtgtat gtcataagat
      481 taaatgttaa ctatgcattc ctcgcggtga attatatgag gtaaaagatg aaaattggaa
      541 ctgtggcagg cactaacgac agtacaacga caatcgcaac aaatgatatg gtgcaagagc
      601 atgttactaa cttcactaaa gaactgtttg ggtatattgc aaatggtata ggtgatgata
      661 tatcaagtat tgccaggaca atgctcggtg aagttgtgga aaaaattgat gattggcaaa
      721 tagaacgttt ccaacaatct attcgagatg acaaaatttc atttaccata caaacgaatc
      781 attcagaaaa atatagtatg ctatcgggca tgcgcgctca tattctacgt agggataata
      841 attatcagtt tattgtgaca attaatagta taaattacgg ctgctatctt gataataccg
      901 atattaattg gtgcagcatt gtttatttac ttaataatat gacagtaaat gatgatgcta
      961 atgatgttgc agttactgag tcgtacaaac ccatctggaa ctggaaaata tcgcaataca
     1021 atgtatttga tattaaattc gaaacaatga taaaacccca gttcgctgac agaacctatt
     1081 tctccaattg ttcacctgtt gatcccacaa gtaccagacc aacctatttt ggcgataccg
     1141 atggctcagt cggtgcagtt ttgtacgcgc tttttgcaac gggacatttg ggaatcatgg
     1201 ctgaaggaga aaatttcctt agccagttgc ttaatataga agatgaagtt ctcaacgtat
     1261 tgctaaggga gaactttaat gagcaacttg acactaatgt taatataata atcagtatcc
     1321 tgaacagaag agacattgtt ttagaaagtc ttcagcctta tttggttatt aataaagacg
     1381 cagtcacgcc atgtacgttt ctgggcgacc agactggcga tcgatttagt aatatctgtg
     1441 gcgataaatt tat
//
LOCUS       DAZGJW010000435         1452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZGJW010000435 DAZGJW010000000
VERSION     DAZGJW010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.439"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            80..205
                     /locus_tag="ACHKIK_005536"
                     /pseudo
     CDS             80..205
                     /locus_tag="ACHKIK_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            317..807
                     /locus_tag="ACHKIK_005537"
                     /pseudo
     CDS             317..807
                     /locus_tag="ACHKIK_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Ia central receptor-binding
                     domain-containing protein"
     gene            complement(829..1119)
                     /locus_tag="ACHKIK_005538"
     CDS             complement(829..1119)
                     /locus_tag="ACHKIK_005538"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HFO9086450.1"
                     /translation="MGGYTLYMSWDYEFKYRLLFWCISLCGMVLYPVARWCIENTALK
                     FTQPHFWNSGFFADTPGKMGLLAVYTGTVFILSLPLTLIYILSVIIKRLSVR"
     gene            complement(1283..>1452)
                     /locus_tag="ACHKIK_005539"
     CDS             complement(1283..>1452)
                     /locus_tag="ACHKIK_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO9086451.1"
                     /translation="SGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPANRVRD
                     LLPWKVDLSSQ"
BASE COUNT          440 a          318 c          320 g          374 t
ORIGIN      
        1 gtgtggcggc ctcagtccgg aacaatttga aaaccagaac ctcgcttagg cctgtgtcca
       61 tattacgtgg gtaggatcag ttgttcctgc ttctgagctc gcagaagcac tgaaacaggt
      121 tcgggaaatc ccgcgccttc tgggtaagaa gacgatggaa gttgagatcc tgaaagaagc
      181 catggagtac ggcctgtcgc gaaaatggat agcgcacacg cctttgttgc caaaggacgg
      241 gaaacggcag aaggatgcac agagtgcaga caaaaaggcc gctgatatgc ttgctaaata
      301 tgagcgcaga aaaggtattg ttgtgagtgg tgctccgaga tttgccggta cgataaaaat
      361 caccacaaac tcggtcatcg ataaccgtgc aaacctgaat tatcttctga ctcattcctg
      421 tctggactat acatgtaata ttctgagtga ctgaaatcca ctgctgacag agagtgtgga
      481 aggtgacaaa aaatttataa tgctgcagtt gctgaatggg ataagttaca gcaacgattg
      541 cttgatgcca gaaataaaat ttacaatttt tgctgactgg atcactgagt ttggtaaggc
      601 tgtccggaca gataactggc gtcctttttt tgttaaaaca gaaaccatca tagcaggtaa
      661 tgctgcaacg gctcttgttg cactggtctt cagtattctt cccggaagca ctttaggcat
      721 tattgggtat ggtttactga tggctgtcac cgatacactg attgatgaaa cgcctgtgga
      781 aaaagcgaat agttctgggg tatttaaata atatataaga caggctgtct atcttacaga
      841 cagccttttt atgataacag aaagaatata tatcagagtt aacggaagag ataatatgaa
      901 aacagtaccc gtataaaccg caagtaatcc catttttccg ggagtatcag caaaaaaacc
      961 gctgttccag aaatgaggct gggtaaattt cagggcagta ttttcaatac accatctggc
     1021 aaccggatac aaaaccattc cgcagagaga aatacaccag aacagcaatc tgtatttaaa
     1081 ctcataatcc catgacatat acagagtata cccacccata aaccatccca ccacatatta
     1141 ttaagataat atttcctgtt catcagccag cctcctgttc tccctttctt ttgtcagtgt
     1201 accggtgcat gacaacataa tcacatcatg ataataataa tcaataacaa tagtaagcga
     1261 ctcgtcagaa ccgtattgat atttactgag agctcagatc aactttccag ggcaacagat
     1321 cgcgtacccg gtttgccggc cagtcctgga tatgttcaat gacgtaacgc agccactttt
     1381 ctggctccac attgttcaga cggcatgtgc cgatcagcga gtacaacacc gccgcatgtt
     1441 caccaccgct gt
//
LOCUS       DAZGJW010000436         1425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZGJW010000436 DAZGJW010000000
VERSION     DAZGJW010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.440"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1425
                     /locus_tag="ACHKIK_005540"
     CDS             <1..>1425
                     /locus_tag="ACHKIK_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO9086452.1"
                     /translation="TDMMPAYSKQVIFKIMMQTNGDNRFLEDLKLRRAISDGVLRYVN
                     NQNIDEVNYNEISDADKNIIKKILTEISKMPEDSIFTKINTRIPRDTMPILRRYHLWP
                     DGWNIRGLNGFMLSHKGSEVIDAVIAGQNQAYRELRRIRDNIHSEIYFKQTDELSSLP
                     DTDKIGGILVKKYLSGSLFSKFRQDTIIPEALSTLQISGPDLIQRKMLQFFRSRGVLG
                     EEFINERKLSDKAYIGVYKTTGTGKYDWLNPESIGVNDVTPADESTWCIGKGRCVDDF
                     LFKDVSKLKTENLPELFLTKIDTDTFFSQWSTKTKKDLQKKIQDLTVRYNELIDSSTI
                     DFKNLYEIDQMLHMIMLEMNDDIAKRSLFSLQVQIAEKIRRMTIPVDNIINIYPDLHK
                     KNDNDLSMSIKGFLASNPHTKINILYSNKTEHNIFIKDLFSFAVMENELRDIINNMSK
                     DKTPENWEGRVMLQRYLELKMKDHL"
BASE COUNT          534 a          227 c          267 g          397 t
ORIGIN      
        1 cacagacatg atgcctgcat actctaaaca agtaattttt aaaattatga tgcagacaaa
       61 cggagataat cgtttcttgg aagatttgaa actacgtcgt gcaatatctg atggtgtatt
      121 aagatatgtt aataaccaaa atattgatga agttaactat aatgaaatca gtgatgcaga
      181 taaaaacatt atcaagaaga tattaacaga aatatctaaa atgccagaag atagcatttt
      241 tactaagatc aatacaagga ttcctcgaga cacaatgccc atccttcgtc gttatcacct
      301 atggcctgat ggatggaata ttcgtgggct caatggattc atgctatcac ataaaggtag
      361 tgaagtgatt gatgctgtta tcgcaggcca gaatcaggct tacagggaac taagaagaat
      421 aagagataat attcatagtg aaatatactt caaacaaact gatgaattgt cctcacttcc
      481 agatacagac aaaattggag ggattctggt aaaaaaatac ctttcaggaa gtcttttttc
      541 aaaattcaga caagatacta ttatcccgga ggcattgagc accctccaga tatcaggtcc
      601 tgacttgatt caaagaaaga tgttgcaatt tttcaggagt agaggggtgt taggtgaaga
      661 gttcattaat gaaagaaaac tgagtgataa agcttatatt ggtgtctaca aaacaactgg
      721 cacagggaaa tatgactggt taaaccctga atcgatcggc gttaatgatg tcacgcccgc
      781 agatgaaagt acctggtgta taggaaaagg ccggtgtgtt gatgacttcc tgttcaaaga
      841 tgtttcaaaa ctaaaaacag aaaatcttcc agaattattc ttaacaaaaa tagatactga
      901 tacgtttttc tctcagtggt caaccaaaac caagaaagat ctgcaaaaaa aaatacaaga
      961 ccttactgta cgttataatg agctaattga ctcgtcgact atcgacttta aaaatctata
     1021 tgaaatagat caaatgctcc atatgattat gctagagatg aatgatgata tagccaaaag
     1081 gtcattgttt tcattgcaag ttcaaatagc cgaaaaaatt cggaggatga ccattcctgt
     1141 agacaatata attaacatct atcctgatct acataaaaaa aatgacaatg atctgagtat
     1201 gtccataaaa ggctttcttg cgagtaatcc acatacaaaa ataaatattc tttatagcaa
     1261 taagactgag cataatattt ttataaagga tttattctcc ttcgcagtta tggaaaatga
     1321 gttaagagac attatcaata acatgagcaa ggataagact cctgaaaact gggaagggag
     1381 ggtaatgtta caaagatatc tcgaattaaa aatgaaagat catct
//
LOCUS       DAZGJW010000437         1411 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZGJW010000437 DAZGJW010000000
VERSION     DAZGJW010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.441"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1051)
                     /gene="tssI"
                     /locus_tag="ACHKIK_005541"
     CDS             complement(<1..1051)
                     /gene="tssI"
                     /locus_tag="ACHKIK_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO9086453.1"
                     /translation="MSTGLRFTLEVDGLPPNAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPA"
BASE COUNT          338 a          375 c          364 g          334 t
ORIGIN      
        1 ctgccgggat caccgcaaaa tggttctcca gcgccgtccc cgctccctgc cgtcctggca
       61 ccgcctgtgg ctgttcgccg tgcagttcac ttgccaccac ctgccattcc cggttcaggt
      121 tcgcctgcgg atgccccgtc agcacaattc gtcgtcccgg ccatatctcc ggcgagcgac
      181 tcattccccg cgcggtttct gcattgtttc gccagccgtc catctgccag cgggcaaagt
      241 tctgcccgtg ggcccccttg aaacgtccgg ggtagtcata cacttcatac tgtgtgcgct
      301 ggtaatcctg gtgctggcct tcctgatcaa aacgtccggc ccagccgggg cgtttaaagg
      361 tgtagtcttt ggtcaccacg gaagaagggc ggatttgtgc gctgtagcgg aactggctga
      421 tgcagagggt gctcacctcg gtacgggtgt tcgggttcca tgggatttca aaagattcgg
      481 gcagatggcg gactgtgtcg cacagcacca ggctctggtc ggtacttttg taagcatgct
      541 cctcataaaa gaagatgcct tcctccgccg ccatccggca caggaaatcg taatcagtct
      601 caccgtactg gacacaaaac tcacgggaag gatgcggctc gctgaacagc ggactccatt
      661 cggtcacccc gttttcctgc aacatcgtgc cgaggatgct tttgatgtcc tcgttctgga
      721 agatacggaa gttctggcgc agaccggcac gccacagcgg cgggtgcacc ttcatgctgt
      781 acagcatctg gtttttgtcg ttctccccga gttcaaacca ggtcaccacg cctttcaccc
      841 ggcgctgtac ttcatcgccc tgccatatcg tcaggtaggc cattttgtcc agcacctgcg
      901 caaattcaag ggagagaaac tgctggctga ccagggagag atcgagggaa aagagcgaag
      961 agagtgactg gttcagatga aaggaaacca ccgcaaaagc attcggcggc aggccgtcca
     1021 cttccagtgt gaaacgtaat ccggttgaca tagcaacctc cgtgaaaatg aataataatc
     1081 cgcacatatt gcatgtacaa catgtcgggc ggatgagata agcgagtcga catctggcgt
     1141 taccggggaa tatcagaaaa atgtcatggt tatgtgatga tttagtctgg gattttactt
     1201 cagagaaacg ggtgtctgtt tagtcaacaa aataatcaca aacgcaacgg atgggttctc
     1261 gacgttattc acgacaggct cgtacattac agcgagtaat aaaaaacctg atatgcaaag
     1321 tcaaaccaat attcataatg tctttttatt cacaacaata aataattcaa taacaatcat
     1381 tgaaatgaat gatggtttgt ataaggaata t
//
LOCUS       DAZGJW010000438         1401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZGJW010000438 DAZGJW010000000
VERSION     DAZGJW010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.442"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..93
                     /locus_tag="ACHKIK_005542"
     CDS             <1..93
                     /locus_tag="ACHKIK_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO9086454.1"
                     /translation="LMTMQKQVEGAQDYIRTQCTKQAFYYPEDV"
     gene            90..314
                     /locus_tag="ACHKIK_005543"
     CDS             90..314
                     /locus_tag="ACHKIK_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086455.1"
                     /translation="MKKLQVTVKPFQGTIPFRVLQHGRVLLEEVFRGKCTECYSRTYE
                     VNATHEEFTVECVMNTDKCRMVSAELQPVC"
     gene            complement(683..910)
                     /locus_tag="ACHKIK_005544"
     CDS             complement(683..910)
                     /locus_tag="ACHKIK_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO9086456.1"
                     /translation="MSSKNRPRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYALRWLFI"
     gene            952..1317
                     /locus_tag="ACHKIK_005545"
     CDS             952..1317
                     /locus_tag="ACHKIK_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO9086457.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNVLKGEQRRVSGSARVSLKRRIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
BASE COUNT          348 a          299 c          377 g          377 t
ORIGIN      
        1 ttgatgacaa tgcagaagca ggtggaaggg gcacaggact atatccgcac tcagtgtact
       61 aagcaggctt tttattatcc ggaggatgta tgaagaaatt acaggtaacg gtaaaacctt
      121 ttcagggaac aattccgttc cgtgttttgc aacatggccg tgttctgctt gaagaggtgt
      181 tcagaggtaa atgcactgaa tgttattcac gaacatatga agtgaatgcc acgcatgaag
      241 aattcaccgt tgagtgtgtg atgaatactg ataaatgccg aatggtatcc gctgaattac
      301 agccagtgtg ttgagcgacc ttattatcca tgcgcggtat tgtcgccgta ttcctgcatt
      361 aacagagacc gcagcccgac agggagactc ctctgcgaga gtgtgcgggg ataatcaaaa
      421 acgatacaca ccggggttta ccgcgtaaac ggagcgcggc gttctcccct catggtcgcc
      481 cgtccggtgc gatggtggaa gaaactggaa tctgttcaat aaaaaaactg ccgtgttgga
      541 gtcacagcag taatgtactg attgggtaga agattattat tgttatgctt tatttttatt
      601 ctatatggct gattatttca attcggaatt aatacagcta atgtctgtga ttttttataa
      661 attcagcaat ataaagaaat agttatatga acagccatcg cagagcatac tgtgtatcat
      721 tcttttttat agtcaactga cgggcatatt ttatgtctgc tgccagctcc cggcggcaag
      781 attcaatgac ccacgcagaa aaattttctg aacctttctg gtcaagagcg atgttaattt
      841 gttcaatcat ctggtttgga aatcggatgt tgcgggttgt tgttctgcgg ggccggtttt
      901 tcgatgacat tttctttcct ctggtgacaa gctatatggc gaggatttta catggctgtg
      961 cttcgtacgt taccgggcag aatcaaaact ctgaataccc ggcgggtgaa tgtcctgaag
     1021 ggtgaacagc gtcgggtcag tggcagtgcc cgggtttccc tcaagcgtcg tatctggctg
     1081 agggatgccg ggcagtgctg tctctgtggt cgtgtggttg acctctgtga cagtgaactc
     1141 gatcaccgaa ttgcacttca gttcggtggt ggtaatgagg agacgaatct ctggacgctc
     1201 tgtaccgaat gccatcgaca aaagtctgct cgtgaagcgg cgggtggtat gccggacccg
     1261 acgctgccgg aggtgtccgg aggtagtggc agagcggacg acatcatcgg actgtaaccc
     1321 gaccgggggg gtatcatccg gcgtaaaaaa cgatcgctcc ggacaccgcc ccccctctca
     1381 cgcagagaaa aaattcccgt t
//
LOCUS       DAZGJW010000439         1396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZGJW010000439 DAZGJW010000000
VERSION     DAZGJW010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.443"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..159
                     /locus_tag="ACHKIK_005546"
     CDS             <1..159
                     /locus_tag="ACHKIK_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO9086458.1"
                     /translation="TGYWFLQLLDRVSPSQWAAIGVLGSLLFGLLTYLTNLYFKIRED
                     RRKAARGE"
     gene            164..697
                     /locus_tag="ACHKIK_005547"
     CDS             164..697
                     /locus_tag="ACHKIK_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9086459.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKGKCDQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            857..994
                     /locus_tag="ACHKIK_005548"
     CDS             857..994
                     /locus_tag="ACHKIK_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086460.1"
                     /translation="MFDFSKLIREIRVMAEKLSTWKYILIWLVFVIMASGYFIGQIRW
                     W"
     gene            996..1334
                     /locus_tag="ACHKIK_005549"
                     /pseudo
     CDS             996..1334
                     /locus_tag="ACHKIK_005549"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            1306..>1396
                     /locus_tag="ACHKIK_005550"
     CDS             1306..>1396
                     /locus_tag="ACHKIK_005550"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO9086461.1"
                     /translation="MKPPAPPAWIMQPAPDWQTPLNGIISSSEN"
BASE COUNT          344 a          323 c          412 g          317 t
ORIGIN      
        1 acgggctact ggttcctgca gttgctggac agggtttccc cgtctcagtg ggcggcaata
       61 ggcgtgctgg ggagtctgct gtttggtctg ctgacgtacc tgacgaacct gtatttcaaa
      121 atcagagagg acaggcgtaa ggcggcacgg ggagagtaag ctgatgagca ggaaactccg
      181 ctatggttta tcggctgccg ttctggcgct gattgccgca ggtgcttctg cgcctgaaat
      241 cctcgaccag tttctggatg aaaaggaagg caaccacacc acggcatacc gtgatggtgc
      301 gggtatctgg accatctgcc gtggagccac ccgggtggat ggtaagcctg ttattcctgg
      361 catgaagctg tcgaagggga aatgcgacca ggttaacgcc attgagcgtg ataaggcgct
      421 ggcatgggtg gagaaaaaca tcaaagtgcc actgactgaa ccccagaaag cgggtattgc
      481 gtcattctgt ccttacaaca ttggcccagg taagtgtttc ccgtcgacgt tttataaacg
      541 aattaatgca ggagatcgaa aaggtgcctg cgaagcgatt cgctggtgga ttaaggacgg
      601 tggcagagac tgccgtattc gctcaaataa ctgttatggt caggtctcac ggcgtgacca
      661 ggagagcgcg ctggcgtgct ggggaattga cagataagca gaatattttg ctgaaaaatg
      721 cggtttgctc acacggacgg ataacacaaa atcctgcgaa ctgacaaaaa ctaagtgaat
      781 aaaagtaaaa accccatttg ttggctgcaa gcggggtttt gtgtttcctg actccggaaa
      841 agtcaaagga gaaagtgtgt ttgattttag caaactgatt cgggagattc gagtgatggc
      901 tgaaaaatta tcaacctgga agtacatcct tatctggctg gtgtttgtga ttatggcctc
      961 cggttatttc atcggtcaga tacgctggtg gtgaaatgaa ccgcgtactg tgcgtggtca
     1021 tcattgccct gctggtggcc tgtggtgcgc ttagtctggg gctgaatcat taccgcgata
     1081 acgccatcac ctacaaagcg cagcgcgata aaaaagccag agagctggag ctggcaaacg
     1141 caactattac tgatatgcag gtgcgccagc gcgatgttgc tgcgctcgat gcaaaatact
     1201 cgagggaatt agccgatgcg agagctgaaa atgaaactct gcgcgctgat gttgccgctg
     1261 gtcgtaagcg cctgcggatc aacgccacct gccccggtac cgtgcgtgaa gccaccggca
     1321 cctccggcgt ggataatgca accggccccc gactggcaga caccgctgaa cgggattatt
     1381 tcatcctcag agaacg
//
LOCUS       DAZGJW010000440         1394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZGJW010000440 DAZGJW010000000
VERSION     DAZGJW010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.444"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            149..1369
                     /gene="escD"
                     /locus_tag="ACHKIK_005551"
     CDS             149..1369
                     /gene="escD"
                     /locus_tag="ACHKIK_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFO9086462.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTIGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADQSLPVGKVIDIAGCCFIIGDIDHTLP
                     LSDVPERFSAKNRRKKRLILASVIGAAIALSGAIGSYVLLSPKAEPPAFTRADVYQQL
                     KENKLHAITLVWHGKNVALYGRCESTTDLTPFFNYLKEKNIFYYNNIICNNQIISAIN
                     DILTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLLLNTPGVAGWEIHNNSNN
                     KINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKILAIINAMNKNSNVKILF
                     QNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGIN
                     ISRPDEYIHIPLSY"
BASE COUNT          445 a          281 c          257 g          411 t
ORIGIN      
        1 agccattgga aactcacgta atcattaatt atgaatattc cattaaatag tactgtacaa
       61 cagcttcctt tctactctat tcttagttga tgtaagttca ccatattttt tctcattgtt
      121 caaccaccag gatgaataaa atttaaaaat gttatcctca tataaaataa aacttctcaa
      181 tggtgcgatg cgtaatcggg aattacaact tccgatggga aacctaacga taggcacaga
      241 agataacgat attgtttatt ttccccttga acaaggcctc aatcagtttt tacttgatat
      301 acgtgaggaa ggtgttttcc ttttatctcc ggttgaattt tggattgatg gacagcccac
      361 cccctatgaa gcagatcagt cattacctgt cggaaaggtc atcgatatag cgggatgttg
      421 cttcatcatt ggtgatattg accacacctt gccattgtct gatgtgccag aaaggttctc
      481 tgccaaaaat cgacgtaaaa agcgtcttat cctggctagc gtgattggtg ctgcgattgc
      541 gttgtctggc gccatcggca gttacgtttt gctttcccca aaagctgaac ccccagcgtt
      601 taccagagct gatgtatacc agcagctaaa agaaaacaaa cttcatgcaa tcactctggt
      661 atggcatggg aaaaatgtcg cgctttatgg gcgttgcgaa tcaaccactg atttaactcc
      721 attttttaat tacctgaaag aaaaaaatat tttttattac aacaatatca tttgcaataa
      781 ccaaatcatc tccgcgatta atgatatttt aacggaatac ggttataagg atatcatcat
      841 tacaaaaggt aataagcctg gattcttcct gttatcggga tacattcccc cttcaccaaa
      901 atggagcgaa gttgaaaatc tgttactgaa tacgccaggg gttgctggat gggaaataca
      961 taacaacagt aacaataaaa tcaatgaact ggcatcagag ttcaaaaaga ataaattaat
     1021 aaactatgtc aatattttca aaaagaatga tgtcatcatt gttgcggggg aggtttctca
     1081 acagaacgag agtaagatcc ttgccattat aaatgcgatg aacaaaaata gtaacgtcaa
     1141 gatactattt cagaacattc agccgtacat ttcagcagat attttccccg gaaaaatact
     1201 cagaatcagc ggcacgatga agaatccaac aattgctctt gataacggta caagcctggg
     1261 aattggtagc attctgaaag gaggctatgt catagacgcg attgatccta aagatggtat
     1321 caacatctcc cgcccagacg aatatataca tattccgttg tcttattaac ttattattat
     1381 cttccccctc ttcg
//
LOCUS       DAZGJW010000441         1387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZGJW010000441 DAZGJW010000000
VERSION     DAZGJW010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.445"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1387
                     /locus_tag="ACHKIK_005552"
     CDS             <1..>1387
                     /locus_tag="ACHKIK_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO9086463.1"
                     /translation="HAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVV
                     DLARARANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGS
                     IHRWAKKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNK
                     YICEYLTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVERE
                     GPFYFLFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTA
                     EDSEQPEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQ
                     TRDSVNQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKACTACGQTGGGN
                     CPDCGAVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGE
                     TADHPIKVNGH"
BASE COUNT          448 a          329 c          355 g          255 t
ORIGIN      
        1 tgcacgccat tgggtgggtt aagcataaat gtattcctgg tgctaaatgg cccgaaattc
       61 aggcagagat gcgcatctgg aaaaaacgtc gcgaaggtga acgcaaggaa accggaaaat
      121 acacgtctgt tgttgatctc gcccgcgcca gagccaatca acagtacact gaaaattcaa
      181 caggaaaaat cagcccggtc attgctgcca ttcatcgcga atacaagcag acatggaaaa
      241 cactggatga cgaactggcc tacgctctct ggcctggtga tgtggatgcc ggaaacattg
      301 acggcagcat ccatcgctgg gcaaaaaaag aagttatcga caacgaccgc gaagactgga
      361 agcgtatctc ggcatcaatg cgcaaacagc ctgatgccct tcgctacgac cgccaaacta
      421 tttttggcct tgtccgtgag cgtccgatcg acattcacaa agatcccgta gcactgaaca
      481 aatatatctg cgaatacctg acgacaaagg gcgtgtttga gaatgaagaa acagacctgg
      541 gcactgttga tgttctccag tcatcagaaa cacaaactga tgcagtggaa actgaggtat
      601 ctgatatccc aaaaaatgaa accgcgccgg aagctgaacc atctgtagag cgtgaggggc
      661 cgttctattt cctcttcgca gataaggacg gagaaaaata cggtcgcgca aacaaacttt
      721 ctggtctgga taaggcactg gctgctggcg ccactgaaat cacaaaagaa gaatattttg
      781 cccgaaaaaa tggcacatac acgggcttac cgcaaaatgt agataccgct gaagattcag
      841 aacaaccaga gccgataaaa gttaccgctg acgaagtaaa caaaattatg caggcagcca
      901 atatcagcca gcctgacgcc gataagttgc ttgctgcatc acgtggtgaa tttgttgaag
      961 ggattagtga cccgaatgat ccgaaatggg ttaaggggat ccagacccgc gattctgtga
     1021 accagaacca gcatgaatcg gaacggaact accaaaaagc ggaacaaaac agtccaaatg
     1081 cgttacaaaa cgagccagaa acgaaacagc ctgaaccagt ggcgcaacag gaagtggaaa
     1141 aagcctgcac cgcctgcggt cagaccggcg gcggcaactg ccctgattgt ggcgcggtga
     1201 tgggcgacgc aacataccaa gaaacattcg atgaagagta tcaggttgaa gttcaggaag
     1261 atgatccgga ggaaatggaa ggcgctgaac atccacacaa ggagaacact ggcggcaatc
     1321 agcatcacaa tagcgataat gaaactggcg agacggcaga tcacccaatt aaggtgaacg
     1381 gtcatca
//
LOCUS       DAZGJW010000442         1371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZGJW010000442 DAZGJW010000000
VERSION     DAZGJW010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.446"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(91..167)
                     /locus_tag="ACHKIK_005553"
     tRNA            complement(91..167)
                     /locus_tag="ACHKIK_005553"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(131..133),aa:Arg,seq:tct)
     gene            complement(265..340)
                     /locus_tag="ACHKIK_005554"
     tRNA            complement(265..340)
                     /locus_tag="ACHKIK_005554"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(305..307),aa:Ile,seq:cat)
     gene            complement(390..>1371)
                     /locus_tag="ACHKIK_005555"
     CDS             complement(390..>1371)
                     /locus_tag="ACHKIK_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO9086464.1"
                     /translation="LPENSVDLIVTDPPYFKVKPEGWDNQWEGDDDYLKWLDQCLAQF
                     WRVLKPAGSLYLFFGHRLASDTEIMMRERFNVLNHIIWAKPSGRWNGCNKESLRAYFP
                     ATERILFAEHYQGPYRPKDDGYVAQGRELKQHVMAPLISYFRDARKSLGITSKQIAEA
                     TGKKNMASHWFGTSQWQLPNEGNYNKLQALFARVAAEKHQRGELEKPHHQLVSTYSEL
                     NRQYTELLSEYKNLRRYFGVTAQVPYTDVWTHKPVQYYPGKHPCEKPAEMLQQIINAS
                     SRPGDLVADFFMGSGSTVKAAMALGRCAIGVELETGRFEQTVREVQDLIV"
BASE COUNT          323 a          379 c          315 g          354 t
ORIGIN      
        1 tttgacaaac atataatatt ttcaacaata tactgagcca ggtatattcc agtataaggc
       61 tctgccggaa gaaatctgga agaatgaata tggcgcgttg tactggattc gaaccagtga
      121 ccgattgctt agaaggcaat tgctctgtcc ggctgagcta acaacgcata atgcagataa
      181 tggattgcca tcggggaccc gggccccaca cagccagttt cgaaagctga cgctctctgc
      241 cgatgagcta atggcggtat gtgatggtgg cccttgctgg atttgaacca gcgacctggc
      301 gattatgagt cgctcgctct caccactgag ctaaagggcc ggtagcagaa taataatggt
      361 gcgtaattaa ttctgcaatc ccatccgttt caaacgatta aatcctgaac ttccctgact
      421 gtctgttcaa aacgtcctgt ctccagctca acaccaatcg cacaacgccc cagtgccatc
      481 gccgctttta ccgttgaacc tgaacccata aaaaaatctg caaccaggtc tcccggacga
      541 ctgctcgcgt tgattatctg ctgcagcatt tctgccggtt tttcgcacgg atgtttccct
      601 ggatagtact gcaccggttt atgcgtccag acatcggtgt acggaacctg cgccgtcaca
      661 ccgaaatacc gccgcaaatt tttatattca ctcagcagtt ccgtatactg ccggttcagc
      721 tcactgtatg tgctgaccag ctggtggtgt ggcttttcca gttccccgcg ctgatgtttt
      781 tctgccgcaa cacgcgcaaa caacgcctgc aatttgttgt aattaccctc gttcggtaac
      841 tgccactgac tggtaccaaa ccagtgcgaa gccatgtttt tctttccggt ggcttccgct
      901 atctgttttg acgttattcc cagtgattta cgcgcatcac gaaagtaaga aatcagcggg
      961 gccatgacgt gctgttttag ctcgcgcccc tgtgccacat aaccatcatc tttcgggcga
     1021 tacggtccct gataatgttc tgcaaacaga atgcgctctg ttgccggaaa atacgcccgc
     1081 agactttcct tattgcaccc gttccagcgt ccggacggct tcgcccagat aatatggttc
     1141 agcacattaa agcgctcacg catcatgatt tcggtgtcag atgccaggcg atgaccaaag
     1201 aacaggtaaa gacttccggc aggcttcagt acccgccaga actgcgccag acactggtcc
     1261 agccatttca ggtaatcatc gtcgccctcc cactggttat cccagccctc gggcttcact
     1321 ttaaagtatg gcgggtctgt gactatcaga tcgacagagt tttccggtaa g
//
LOCUS       DAZGJW010000443         1368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZGJW010000443 DAZGJW010000000
VERSION     DAZGJW010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.447"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            42..1366
                     /gene="nleA"
                     /locus_tag="ACHKIK_005556"
                     /pseudo
     CDS             42..1366
                     /gene="nleA"
                     /locus_tag="ACHKIK_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
BASE COUNT          443 a          288 c          284 g          353 t
ORIGIN      
        1 tgatattatt aatggatata aacatgtaat aaggatttat tatgaacatt caaccgacca
       61 tacaatctgg aatcacctca caaaacaatc aacatcatca aacagaacaa ataccctcta
      121 cacaaatacc gcaatccgaa ttacctctag gatgccaagc tggatttgtt gttaatattc
      181 cagatgatat acagcaacat gcaccggaat gcggtgaaac aacagctcta ctgagcttga
      241 taaaagataa aggtctgctc tcagggctag acgaatatat agctcctcac cttgaagaag
      301 gatccatagg aaaaaaacat tggatatgtt tggtttattc aatgttaccc aaatggcatt
      361 agagatacct agttccgttt caggcatctc tggtaaatat ggtgtccagc taaacattgt
      421 aaaaccagat attcatccta catcaggtaa ttatttttta cagatattcc ctctgcatga
      481 tgaaataggt tttaatttta aagaccttcc tggcccgtta aaaaatgcat taagcaacag
      541 taatatatca accactgcag tgtcgactat tgcatcgact ggaacatcag ccactacttc
      601 gacggtaacc accgcgtcaa aagacccaat accatggttt ggattaacag ctcaagtagt
      661 tcgtaatcat ggtgtggaac ttcctatagt caaaactgaa aatggatgga agcttgttgg
      721 agaaactcct cttactcctg atggccccaa agcaaattat actgaagagt gggtgatcag
      781 accgggagaa gcagatttta aatatggtgc atctccacta caggcaactc tagggctgga
      841 gtttggcgca catttcaagt gggatttaga taaccctaat actaaatatg ccgttcttac
      901 caatgctgcc gcaaatgcgc ttggtgctgt agggggattt gcagtatcca gatttactgg
      961 tacagatcca atgttaagtc ctcatatcgg tgcaatggtt gggcaagcag cggggcatgc
     1021 catacagtat aatacccccg gattaaagcc agacactatt ttatggtggg caggtacgac
     1081 tctggggctg gcagatttaa acaaagccga gacaggtgaa ggcagattca ctgactatcc
     1141 tcgtatatgg tggcacgcaa gagaaggtgc catcttccct aataaagaag atattgcccg
     1201 tgcaacaggg gctgatatac gagctatgga agaaggtata cccgttggac agcgacatcc
     1261 aaatccagag gatgtggtca tcgatatcga aagcaatggc tcaccacatc ataacccatc
     1321 aaattatgtt gatacctttg atataatcca agaaacaaga gtctaagc
//
LOCUS       DAZGJW010000444         1365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZGJW010000444 DAZGJW010000000
VERSION     DAZGJW010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.448"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1365
                     /locus_tag="ACHKIK_005557"
     CDS             <1..>1365
                     /locus_tag="ACHKIK_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO9086465.1"
                     /translation="ITITDSLNRREVLHTQGEAGLKRVVKKEHADGSVTQSQFDAVGR
                     LKAQTDAAGRTTEYSPDVVTGLTTPDGRASAFYYNHHNQLTSATGPDGLELRREYDES
                     GRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVT
                     RYDHDRFGQVTAVHREEGLSQYHAYDSRGQLTAVKDTQGHETRYEYNIAGDLTAVIAP
                     DGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQ
                     ETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWYYDEADRLTHRTVKGETAERWQYDERG
                     WLTDISHISEGHRVAVHYGYDSKGRLASEHLTVHHPQTEALLWQHETRHAYNAQGLAK
                     RCIPDSLPAVEWLTYGSGYLAGMKLGDTPLVEYTRDRLHRETLRRFGRYELTTAYTPA
                     GQLQR"
BASE COUNT          330 a          411 c          413 g          211 t
ORIGIN      
        1 catcaccatc accgacagcc tgaaccgccg tgaagtgctg cacacgcagg gcgaagccgg
       61 gctgaagcgg gtggtgaaaa aggaacacgc ggacggcagc gtcacgcaga gtcagtttga
      121 cgcggtgggc aggcttaagg cacagacgga tgccgcaggc cggacaacag aatacagccc
      181 ggatgtggtg acgggcctca ccacgccgga tggcagggca tcggcgtttt actataacca
      241 ccacaaccag ttaacgtcag ccaccgggcc tgacgggctg gaattgcgcc gggaatatga
      301 tgaatccggc cgtctgattc aggaaactgc ccctgacggc gatatcaccc gctaccgtta
      361 tgataatcca cacagtgact taccctgcgc aacggaagat gccaccggca gccggaaaac
      421 catgacgtgg agccgttacg gtcagttgct gagcttcacc gactgttccg gttatgtaac
      481 ccgctatgac catgaccgtt ttggtcaggt gacggcggtg caccgcgagg aaggcctgag
      541 ccagtaccac gcatacgaca gccgtggaca gttaactgcg gtgaaagaca cgcagggcca
      601 tgaaacgcgg tatgaataca acatcgccgg tgacctgacc gccgtcattg ccccggacgg
      661 cagcagaaac gggacacagt acgatgcgtg gggaaaggcc atctgtacca cgcagggcgg
      721 gctgacgcgc agtatggaat acgatgctgc cggacgggtc atccgcctga ccagtgaaaa
      781 cggcagccac accaccttcc gttacgatgt actcgaccgg ctgatacagg aaaccggctt
      841 tgacggccgc acacagcgtt atcaccacga cctgaccggc aaacttatcc gcagcgagga
      901 tgaggggctg gtcacccact ggtactatga cgaagcagac cgcctcactc accgcaccgt
      961 gaagggtgaa accgcagagc ggtggcagta tgacgaacgc ggctggctga cagacatcag
     1021 ccatatcagc gaagggcacc gggtggcggt gcactatggt tatgacagta aaggccgcct
     1081 cgccagtgaa cacctgacgg tgcatcatcc gcagacggaa gcactgctct ggcagcatga
     1141 gaccagacat gcgtacaacg cgcagggact ggcgaaacgc tgtataccgg acagcctgcc
     1201 cgccgtggaa tggctgacct acggcagcgg ttacctggca ggcatgaaac tcggcgacac
     1261 accgctggtg gagtacaccc gcgaccgcct gcaccgggaa acgctgcgcc gcttcggccg
     1321 ttatgaactc accaccgctt atacccctgc cgggcagtta cagcg
//
LOCUS       DAZGJW010000445         1363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZGJW010000445 DAZGJW010000000
VERSION     DAZGJW010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.449"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1363
                     /locus_tag="ACHKIK_005558"
     CDS             <1..>1363
                     /locus_tag="ACHKIK_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO9086466.1"
                     /translation="VEGPVKGLQSILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPP
                     EGFESSGSETALGVEVTKAKPVTRTITSANIDRLRVTFGVQSLLETTSKGDRNPSSVR
                     LLIQLQRNGNWVTEKDVTINGKTTSQFLASVILDNLPPRPFNIRMVRETADSTTDQLQ
                     NKTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPE
                     KRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQ
                     TVPDGFGGTEPRMTFNAYLSQQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVV
                     WPYTNCDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRY
                     GRNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIEICDN
                     DYAG"
BASE COUNT          306 a          370 c          436 g          251 t
ORIGIN      
        1 cggtggaagg tccggtgaag gggctgcaga gtattctggt gaacaaaacc ccgctgacgg
       61 acacggacgg taatcctgtg atacatggtg tgacagcggt ctggcgcgcc ggggagcagg
      121 agcagacacc gccggaaggt tttgagtcat ccggctctga aaccgcactg ggcgtggaag
      181 tgacgaaggc aaagccggtg acgcgcacca ttacgtccgc gaacattgac cgcctgcggg
      241 tcaccttcgg ggtgcagtca ctgttggaga ccacctcaaa gggcgaccgt aatccctctt
      301 ctgtccgact gctgattcag ttgcagcgta acggtaactg ggtgacggaa aaggatgtca
      361 ccattaacgg caagaccacc tcacagttcc tcgcttcggt gattctggat aatctgcctc
      421 cccgcccctt taacatccgg atggtcaggg agacggcgga cagcaccacg gaccagctgc
      481 agaataagac gctgtggtcg tcatacaccg aaatcatcga tgtgaaacag tgctacccga
      541 acacggccat tgtggggctg caggtggatg cggagcagtt cggcggccag cagatgacgg
      601 tgaactacca tatccgcggt cgcattattc aggtgccgtc aaactatgac ccggaaaaac
      661 gcacgtacag tggtatctgg gacggcagcc tgaaaccggc atacagcaac aacccggcct
      721 ggtgcctgtg ggacatgctg actcacccgc gctacggcat gggaaaacgt ctgggggcgg
      781 cagacgtgga caaatgggcg ctgtatgcca tcgggcagta ctgcgaccag acggtcccgg
      841 atggtttcgg gggcacagag ccgcggatga cctttaatgc gtacctgtca caacagcgta
      901 aggcatggga tgtgctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg
      961 gccagacgct gacgttcgtt caggaccgcc cgtcggatgt ggtgtggccg tacaccaact
     1021 gcgatgtggt ggtggatgat aacggcgtgg gattccgcta cagcttcagt gccctgaagg
     1081 accgccacac ggcggtggag gtgaattaca ccgacccgca gaacggctgg cagacctcca
     1141 cggaactggt ggaagacccg gaagccatac tgcgctacgg gcgcaacctg ctgaagatgg
     1201 atgcgttcgg ctgcaccagt cgcggtcagg cccaccgtgc cgggctgtgg gtgataaaga
     1261 cagaactgct ggaaacgcag acggtggatt tcacgctcgg gtcacagggg ctgcgtcaca
     1321 cacccggtga cattattgaa atctgtgata acgactatgc cgg
//
LOCUS       DAZGJW010000446         1351 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZGJW010000446 DAZGJW010000000
VERSION     DAZGJW010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.450"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            95..487
                     /gene="ydeI"
                     /locus_tag="ACHKIK_005559"
     CDS             95..487
                     /gene="ydeI"
                     /locus_tag="ACHKIK_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO9086467.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            742..>1351
                     /gene="dgcZ"
                     /locus_tag="ACHKIK_005560"
     CDS             742..>1351
                     /gene="dgcZ"
                     /locus_tag="ACHKIK_005560"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO9086468.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDSFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVY"
BASE COUNT          378 a          262 c          313 g          398 t
ORIGIN      
        1 atgccttata acaattggca gtattcttat ttggcaatta taaaaatagc aaccagactt
       61 aataagtcaa accaaacgca attaaggagt tagtatgaaa tttcaggcca ttgtattagc
      121 aagttttctt gtcatgcctt atgcgttggc agatgaccag ggcggtttaa aacaggatgc
      181 agcgccaccg ccgcctcatg caatagaaga tggctatcgc ggaaccgatg atgcaaaaaa
      241 aatgaccgtt gatttcgcaa aaaacatgca cgatggcgct tcggtttcac tacgcggtaa
      301 tttgatttct cacaaaggag aggaccgtta cgtttttcgc gataagagcg gtgaaattaa
      361 tgtcgttatt cctgcggccg tctttgatgg acgagaagtt cagccggacc agatgatcaa
      421 cattagcggc agtctggata agaaaagcgc gccggccgtc gttcgggtca ctcatttgca
      481 gaaataaaaa taaattagcc tgatggcctg aactactggg ccattagggt attgggttat
      541 gctatttgtc atccttttat ttacgataat gcagaggtga tttacagaat taaacacttt
      601 tttatccgct gattttgttt taaatcacaa tgttatctca gagatgactc ataacttcgg
      661 atttctatga aaatattgac gtaattttgc ttttctctcg ttagaatagc gcgcacaagg
      721 aactgtgaaa aaggagtggc aatgatcaag aaaacaacgg aaattgatgc catcttgtta
      781 aatctcaata aggctatcga tgcccactac cagtggctgg tgagtatgtt tcacagcgtg
      841 gtcgcgagag atgccagtaa gccagaaata acggataacc attcttatgg actgtgccag
      901 tttggtcggt ggattgatca tctggggcca ctcgataacg atgaattacc ttacgttcgg
      961 ctaatggatt ctgcccatca acatatgcat aactgtggtc gggaattaat gctggctatt
     1021 gttgaaaatc actggcagga cgcgcatttc gactcctttc aggaggggtt gctttctttt
     1081 actgcggcat taaccgatta caaaatttat ttgctgacga tccgtagcaa tatggatgtt
     1141 ttgacgggat tgccggggcg tcgggttctt gatgaatcct ttgatcatca gttacgcaac
     1201 actgagcctc tgaatcttta tttaatgttg ttggatattg accgatttaa attggttaat
     1261 gatacctacg ggcatttaat cggcgatgta gtattacgca ccctggcaac ttacttagcc
     1321 agttggacgc gtgattacga aacggtttat c
//
LOCUS       DAZGJW010000447         1345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZGJW010000447 DAZGJW010000000
VERSION     DAZGJW010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.451"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            160..1305
                     /locus_tag="ACHKIK_005561"
     CDS             160..1305
                     /locus_tag="ACHKIK_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EspG domain-containing protein"
                     /protein_id="HFO9086469.1"
                     /translation="MINGLNNNSASLVLDAAIRINSDFKKQWNDMSCAEKLLKVLSFG
                     LWNPTYTRSERQTFQELLTVLEPVSPAPNELGRIYANFADGSSLRISVTNSELVEAEI
                     RTPDNEKILMLLESNEQNRLLQSLPINLHMPYIQVHRALSKMDLTDHKSMHNLLSFTS
                     KLSATLIPHNTQTDPLSGPTPFSSMFMDTFRGLGNAKLSLNGVDIPVDAQKLLRDALG
                     LKDTHSSLARNVINNGISRHHAKQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALMLTHTRISQAREYNVERSLDVPNACINISISQSPDGSIHVASHTGILIMAP
                     EDRPNELGMLTNRTSYEVPQGVKCEIDEMVRTLQPRYGASETYLKNI"
BASE COUNT          446 a          278 c          243 g          378 t
ORIGIN      
        1 tgttcgtcat tagaggagta tttcctctaa tttaaaaaaa taactctcta tacatttaaa
       61 actctcgcta taaatagctt atatattatt gacactatat catgctatat gatatctgtt
      121 gtcgaatcat tcataataaa tgagacaggg gaatctctca tgataaacgg acttaataat
      181 aattccgcat ctttagtttt agatgctgca attagaatta attctgattt taaaaagcag
      241 tggaatgata tgtcatgcgc tgaaaaatta ctaaaagttc ttagttttgg tttatggaat
      301 ccaacgtata ctcgtagtga aaggcaaaca tttcaagagt tgttaaccgt tttagaacct
      361 gtatctcccg cgcctaatga attgggcaga atatatgcta attttgcaga tggctcatcc
      421 ttaagaattt ccgtcactaa cagcgaactt gttgaagccg agattcgcac accagataat
      481 gaaaagattc ttatgctcct ggagtcaaac gaacaaaata ggttattaca atctttaccc
      541 atcaatctcc acatgccata cattcaggtt catcgtgcct tatctaagat ggacctgact
      601 gatcataagt caatgcacaa tctacttagt tttacgtcaa aactatcagc aaccttgatt
      661 cctcataata ctcaaacaga tccgctttcc gggcctacac cattcagctc tatgtttatg
      721 gatacatttc gaggactcgg caatgcaaag ctttcactca atggtgttga tatacctgta
      781 gatgcacaaa aattgcttcg cgatgcacta ggacttaaag acacacattc atcacttgcc
      841 cggaatgtta taaataatgg tatttctcgt catcatgcaa agcaaatagc aagggaaagc
      901 agcggcagcg ataaacagaa agctgaagtt gtggagtttt tatgccaccc cgaagcggca
      961 acagccatat gctcggcttt ctatcaatct ttcaatgtgc cagccttaat gttgacacat
     1021 acaaggatct ctcaagccag agaatacaat gtggaaagat cgttagatgt acctaacgcg
     1081 tgcattaaca taagtatctc tcaatcacca gatggaagca ttcacgttgc cagccatacc
     1141 gggattctga taatggcgcc agaagaccgc cccaacgagt taggaatgtt gacgaacagg
     1201 acctcctatg aagtgccaca aggtgtgaaa tgtgaaatcg atgagatggt aagaacgcta
     1261 caaccaaggt atggcgcatc tgaaacatac ctgaaaaaca tttaaaaata aaaaaatgga
     1321 caccacacac tttatttatc aaaac
//
LOCUS       DAZGJW010000448         1343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZGJW010000448 DAZGJW010000000
VERSION     DAZGJW010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.452"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..547
                     /locus_tag="ACHKIK_005562"
     CDS             <1..547
                     /locus_tag="ACHKIK_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO9086470.1"
                     /translation="KETTSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQV
                     GQKVLDKILNYTNTEMKEAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVP
                     VALVVAGTQMATGHRTMDTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFE
                     NIKQVIDDMATDATMFDVNKK"
     gene            complement(565..1066)
                     /gene="tnpA"
                     /locus_tag="ACHKIK_005563"
                     /pseudo
     CDS             complement(565..1066)
                     /gene="tnpA"
                     /locus_tag="ACHKIK_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1074..>1343
                     /locus_tag="ACHKIK_005564"
     CDS             1074..>1343
                     /locus_tag="ACHKIK_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9086471.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAY"
BASE COUNT          382 a          303 c          321 g          337 t
ORIGIN      
        1 aaaagaaaca acctcagcaa caggtaaacc tgttttacgt tgggtagacc cgagttttga
       61 tcaaagcaaa tatgacagca tcgtctggaa cccaatcact tattatccgg tgccaaaacc
      121 ttctacccag gtagggcaga aagttctgga taaaattttg aactatacca acaccgaaat
      181 gaaagaagcg atagcgcagc gtaaaccact ggttaccacc gctgggccgc gtagtctgat
      241 tttccgtggg gccattaccg gtgtagatac cagcaaagaa gggctgcaat tctatgaagt
      301 ggttcctgtt gcattagtgg ttgcggggac gcaaatggct acaggccacc gtaccatgga
      361 tactcgcctc tattttgaag gtgagctgat tgatgcagcg actaataaac cggttatcaa
      421 agtcgttcgt cagggcgaag gtaaagacct gaataacgaa agtacgccaa tggctttcga
      481 aaatattaaa caagttattg atgacatggc gaccgatgcc accatgtttg acgttaacaa
      541 aaagtagttc cagacgcgcc atcgttagat ggcgcttttt atccggtgtg ccgtaaaacc
      601 ccatccttca gggcggggat ataaggtgcg gttttccacc tgaccaggtg tttgctgttg
      661 ttcaatgtat tggcggatga cggatattgg cgcacctccg cagctactgg caaaatagcc
      721 aggactccac aaaacgcctt tgtagtaata cctgactgca atatctggtc gatctcgtcg
      781 cagtaaccta cccgatacgc ctttgaggct gtttaccaga ctggatatcg ccagtttggg
      841 aggttagtta attaacaaat ggacgtgatc tggttcgcca tccatttcaa ccagttcagc
      901 ttcaaaataa gcacatacat ttgaaaagta agtgcgtagt ttttctgtcg cgtcaaaaaa
      961 tctggcgtcg gtatctggtg acaaagacca ggtgaacatg catcaggaaa acacaatgcc
     1021 ttccacgccg gatatcggtt tctttattca cagaccaaag tatcatttga cctgtgaaac
     1081 gattacaggc atttaaattc cagttaagac caggtggtca acaggagtgt gaaatgaggc
     1141 gcttcgcagg cgcatgtcgt ttcgttttca atcgtgctct ggcacgtcag aatgagaatc
     1201 atgaggccgg taataaatac atcccttacg ggaaaatggc ttcctggctg gttgagtgga
     1261 aaaatgccac tgaaacgcaa tggcttaaag attctccctc acagccattg caacagtcac
     1321 tgaaagacct tgagcgggct tac
//
LOCUS       DAZGJW010000449         1341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZGJW010000449 DAZGJW010000000
VERSION     DAZGJW010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.453"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(61..273)
                     /locus_tag="ACHKIK_005565"
                     /pseudo
     CDS             complement(61..273)
                     /locus_tag="ACHKIK_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            423..1091
                     /locus_tag="ACHKIK_005566"
     CDS             423..1091
                     /locus_tag="ACHKIK_005566"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO9086472.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(1036..1173)
                     /locus_tag="ACHKIK_005567"
     CDS             complement(1036..1173)
                     /locus_tag="ACHKIK_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFO9086473.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(1146..>1341)
                     /locus_tag="ACHKIK_005568"
     CDS             complement(1146..>1341)
                     /locus_tag="ACHKIK_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO9086474.1"
                     /translation="EIKNSLMQVASEHIAPLQDAVDLEIATEEETSLLEAWKKYRVLL
                     NRVDTSTAPDIEWPTNPVRE"
BASE COUNT          392 a          301 c          258 g          390 t
ORIGIN      
        1 gatattactt taatcaaaag ttgacgtgcc agattcgaag taaatttata aaatatcaaa
       61 tcaatataca ttttgcacct tcgagatagt aatgactgtt actgtaacat gagttacaaa
      121 cagcttcata cccagagcta ctgatgtatc aactcgattc aacaataccc gatatttatt
      181 ccatgcctcc agcaacgatc tttcttcctc cgttgcgatt tccagatcta cagcatcctg
      241 cagtggcgca atatactcac tgaattcctg gatgtagaac tgtgtggtga cggtcttcca
      301 gccattcggc tcctgttgta tcgaagcata ccaggctatt tcaatatcgc tatgctgcgg
      361 cagcatttaa ccccttgtaa ttcatcgcca taattgattt aattcacaaa taaaactata
      421 acatggtgaa attaatgaaa aaaaacacag atgatggggc taaaatttac acaccactta
      481 ccctaaagct ttatgactgg tgggttttgg gagtatcaaa tcggcttgca tggggatgtc
      541 ctacaaagga acaccttctt ccacactttc tggaacattt aggtaacaac catctggata
      601 ttggtgttgg aactgggttt taccttactc acgtacctga gagtagtctg atatctttaa
      661 tggatttgaa cgaagctagc ctgaacgcgg catctacaag ggctggggaa tcaaaaatta
      721 aacataaaat tagccatgat gtttttgaac cttatcccgc ggcgttacat ggtcaatttg
      781 attccatttc catgttttac cttcttcact gcctgcctgg aaatatatct acaaaaagct
      841 gtgtaatacg caatgcggcg caggccttaa ctgacgatgg aactctatac ggagccacaa
      901 ttcttggcga tggagttgtg cacaatagct tcggtcaaaa actgatgcgc atttacaatc
      961 agaaaggcat cttttcaaac acaaaagatt ccgaagaagg cttaacacat atactctcag
     1021 agcatttcga gaatgttaaa accaaggttc aaggtactgt agtaatgttt tccgcttcag
     1081 ggaaaaaata gcatccaacc gcagcacgtt cttgcttaag acgtgctgcg gcataatccc
     1141 aatgattact ccctgacagg gttcgtaggc cactcaatat caggtgcagt tgatgtatca
     1201 acacggttca gcaacacccg atactttttc caggcttcca gcaatgaggt ttcttcctcc
     1261 gttgcgattt ccagatctac agcatcctga agtggcgcaa tatgctcact ggctacctgc
     1321 atcaggctgt tttttatttc t
//
LOCUS       DAZGJW010000450         1339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZGJW010000450 DAZGJW010000000
VERSION     DAZGJW010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.454"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..81
                     /locus_tag="ACHKIK_005569"
     CDS             <1..81
                     /locus_tag="ACHKIK_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9086475.1"
                     /translation="AWRHRHWHASAFTGICNDLAAASACT"
     gene            78..659
                     /locus_tag="ACHKIK_005570"
     CDS             78..659
                     /locus_tag="ACHKIK_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO9086476.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGKVVVIGRQG"
     gene            905..>1339
                     /locus_tag="ACHKIK_005571"
     CDS             905..>1339
                     /locus_tag="ACHKIK_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9086477.1"
                     /translation="MGKGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGSETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVQTTSQGDRNPASVRLLIQLQRNG"
BASE COUNT          305 a          340 c          442 g          252 t
ORIGIN      
        1 gcctggcgtc accgccactg gcacgcatct gccttcacgg ggatttgcaa cgatttggcc
       61 gccgcctcag cctgtacgtg aacacggcag cggaagccat ccgggcgctg tcgttacagg
      121 tgccgggatt ccgccgtcag atgaacgaag gctggtacca gatacgtatt cgcggtgagg
      181 acacggcacc ggaggcggtg tacgcccgtc ttcacgaaca gctgggtgag ggaacggtca
      241 tccacattgt gccgcgactg gccggtgcag ggaaaggcgg actgcagatt gtgctggggg
      301 cggcagccat cgtgggctct ttcttcaccg ccggcgcaac gatggcgtta tggggttcag
      361 ccctggcagc cggcggtttt tctgccacca cgatgctgtt ttcactgggg gccagcatga
      421 tactgggcgg tgtggcccag atgctggccc cgaaggcaaa gacgccggag tacaaaagta
      481 cggataacgg taaacagaac acgtattttt cgtcactgga caacatgatt gcccagggga
      541 acccgatgcc ggtgccttac ggtgaaatgc tggttggctc ccggcgaatc tcccaggaca
      601 tcagcacccg tgatgaaggt ggtggcggga aggtggtggt tatcgggcgg caggggtaaa
      661 gcataaaaaa atcccgcagt gtatggaggc tgcgggaaca gaaaatgaag attaaccaca
      721 gggggttttg tttttattgg cccgaaaaaa ctgtaacgcc cgggaatgat atctgccacg
      781 gggcgtacag aaaatgtgaa gaaattcaga aattttattc cgtcatgaca caggcaccct
      841 ccggggtgcc tgtcgttttt ggggcataaa cagattcaga catcagacag gagaggggga
      901 cagagtgggt aaaggtggcg gcagggcgca cacgccggta gaggcaaagg acaatcttaa
      961 gtccacgcag atgatgagcg tgattgacgc cattggtgaa gggccgattg aaggtccggt
     1021 gaaggggctg cagagtattc tggtgaacaa aaccccgctg acggacacgg acggtaatcc
     1081 tgtgatacat ggtgtgacag cggtctggcg cgccggggag caggagcaga caccgccgga
     1141 aggttttgag tcatccggct ctgaaaccgc actgggcgtg gaagtgacga aggcaaagcc
     1201 ggtgacgcgc accattacgt ccgcgaacat tgaccgcctg cgggtcacct tcggggtgca
     1261 gtcactggtg cagaccacct cacagggtga ccgtaacccg gcatccgtcc gcctgctgat
     1321 tcagctgcag cgtaacggt
//
LOCUS       DAZGJW010000451         1331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZGJW010000451 DAZGJW010000000
VERSION     DAZGJW010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.455"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..318)
                     /locus_tag="ACHKIK_005572"
     CDS             complement(<1..318)
                     /locus_tag="ACHKIK_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO9086478.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDKASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEF"
     gene            complement(318..>1331)
                     /locus_tag="ACHKIK_005573"
     CDS             complement(318..>1331)
                     /locus_tag="ACHKIK_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9086479.1"
                     /translation="GFNVGSPQQAGISELVLRAGNGNPKGITGALWQRTLTGFTNFAW
                     VNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPEGLTDGTVYSLY
                     TPSEQFYPPGAPIPWPSDTVPSGYALMQGQAFDKSAYPKLAAAYPSGVIPDMRGWTIK
                     GKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSG
                     STNAAGNHSHRDGRRFNPSVFKDTYQYGYTSSGQNTWGVQGSVGMSTGWLANTSTDGN
                     HSHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIV
                     RLA"
BASE COUNT          344 a          330 c          303 g          354 t
ORIGIN      
        1 aaactcccct tccggcgata accaggtaac attttccggt aacggaccga gttcagaaat
       61 aaataacgcg tcccctgacg ctacgtcata aaccgtttta ccccgatggt cttcaacgag
      121 atgccacgat gccttatcac tgttgaaaac agccacaaag ccagcaggaa tatctggtgg
      181 tgcaatatcg gtactgtttg ctggcagacc tgtatgaggc ggaatatatg cgtcaccttc
      241 accaataaat tcattagttc cggccagcag attataaatt tttatggtcc gtggttgttc
      301 actcattctg aatgccatta tgcaagcctc acaatatagt taaatgcgat gtttttgacg
      361 gtgttttccg cgttaccagc agcgttaacg gtgatggtgt gtccatgtga accaatcgca
      421 acggagtgcg tatgagcacc aataccgaca gtatgcgcgt gtgcacctgc agatgctgct
      481 gtgccggaca gtgaatggct atgattacca tctgtactgg tattcgctaa ccaccccgta
      541 gacataccta ctgagccttg tacaccccag gtattttgac ctgagcttgt ataaccatat
      601 tgataagtat ctttaaaaac actggggtta aatcgacggc catctctatg gctgtgatta
      661 ccagctgcat tcgtgctgcc acttaaacta tgggtatgcg caccagtgtt attcgtggat
      721 ttagtgccgt aatcaaacga cgatgtggtt ttcgtcccca aatccgtact ggatgcgctg
      781 gcgctgtggg tatgcgattt aatgccgtcc tgttcctgag acaatacggc ccgaccactg
      841 gcaggtttgc ccttaatcgt ccagccacgc atatcaggga tcacgcctga cggataagcc
      901 gctgcaagtt tcgggtatgc agatttgtca aaagcctgcc cctgcatcag ggcatagcca
      961 gacggaacgg tatctgatgg ccacgggatt ggtgcgccag gcggataaaa ctgctctgat
     1021 ggcgtataga gtgaataaac tgtaccgtcc gttaaccctt ccggcttatt agcagaatat
     1081 gctggtgacg tatgaatcgt cacgctggca ttactggtat aatcccattg aatatttaca
     1141 ccagtcgcat aatttccgat tgcaacgtaa atatcgtaag tatcaccaga tgtattaacc
     1201 caggcaaaat ttgtaaaccc tgtcaatgtg cgctgccata aagcaccagt aatccccttc
     1261 ggattaccat tacctgcacg taaaacaagt tcagatatac ctgcctgttg aggtgacccc
     1321 acgttaaatc c
//
LOCUS       DAZGJW010000452         1330 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZGJW010000452 DAZGJW010000000
VERSION     DAZGJW010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.456"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..109)
                     /locus_tag="ACHKIK_005574"
                     /pseudo
     CDS             complement(1..109)
                     /locus_tag="ACHKIK_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            86..>1330
                     /locus_tag="ACHKIK_005575"
     CDS             86..>1330
                     /locus_tag="ACHKIK_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO9086480.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATRDPEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLAD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSAWARRGIISDRLATAIL
                     RHAGRVALNAGASSTVSEVRPSS"
BASE COUNT          315 a          327 c          408 g          280 t
ORIGIN      
        1 cgaccagcca ctggcagatg gccatcctgt gatttgattc cgcttccggc tttttaactc
       61 tgttcctcta cacgggagaa attcgatgtc gattaaacat tatgatgttg tcagggcggc
      121 gtcgccgtca gaccttgcgg aaaagctgac acaaaaactg aaggaggggt ggcagccatt
      181 tggcagcccg gtggccatca cgccttatac tctgatgcag gccattgcgg cggaaggtga
      241 tgtcaccaca cctgtgttgg tgaagccgtc ggatggagaa ggcacagtaa tcagcgccac
      301 cagagacccg gagtattact ttgttgtggt tctggcgggg cagtcaaaca gcatggcata
      361 tggtgaaggc cttccgctgc cggagacata tgaccgtccg gacccgcgca ttaagcagct
      421 ggcgcgtcgc agtacggtga caccgggcgg tgtcgcctgt aaatataacg acatcattcc
      481 ggcggaccat tgtctgcatg atgtgcagga catgagccgc cttaaccatc cgaaagcgga
      541 cctgtcaaag ggacagtacg gaaccgtggg gcaagggctg catatcgcca aaaaattgct
      601 gccgtttata ccggcgaatg cgggcattct gctggttccg tgctgtcgtg gtggttcagc
      661 gttcaccacc ggagccgatg gcacatacag tgacgcgagt ggtgcctcgg agaattcaac
      721 ccgctggggt gtggacaagc cgctgtataa ggaccttatc ggtcgaacaa aagcagcact
      781 gaagaagaat ccgaaaaatg tgctgtttgc cgtggtgtgg atgcaggggg aatttgattt
      841 tggcggtacg ccggcaaatc acgcagcaca gtttggtgcg ctggttgata aattccgtgc
      901 agacctggcg gatatggcag gtcagtgcgt cggtggctct gctggcggtg ttccctggat
      961 atgtggagat acgacgtatt tctggaagca gaagaacgaa tccacgtacc agacggtgta
     1021 cggcagctat aaaaacaaaa cggaaaagaa tatccatttc gtaccgttca tgaccgatga
     1081 gaacggggtg aatgtgccga cgaacaaacc ggaagaagac ccggacattc cgggtatcgg
     1141 atattacggt tcgaaatggc gtgacagctc agccacctgg acgtcacagg acagggcgag
     1201 ccatttcagc gcctgggcac gccgtgggat tatttccgac cgtctggcaa cggcgatttt
     1261 gcgccatgcg ggaagagtgg cgctaaacgc gggggcatca tcgacagtat cagaggtgcg
     1321 cccttcatcg
//
LOCUS       DAZGJW010000453         1329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZGJW010000453 DAZGJW010000000
VERSION     DAZGJW010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.457"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..911)
                     /locus_tag="ACHKIK_005576"
     CDS             complement(<1..911)
                     /locus_tag="ACHKIK_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO9086481.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALAYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQASESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADRTARA"
     gene            complement(892..>1329)
                     /locus_tag="ACHKIK_005577"
     CDS             complement(892..>1329)
                     /locus_tag="ACHKIK_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO9086482.1"
                     /translation="FALRLAREMGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLD
                     MHFSGLTYAVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVR
                     FGGDGGRDILSSADVADVMVDDAALMMASAGISGGVRYVPAGW"
BASE COUNT          291 a          438 c          343 g          257 t
ORIGIN      
        1 gcccgtgcag tcctgtctgc ccaggtctcc agcgtgccca tgttctcttt caggcggcgg
       61 gtctgctcat caaagccttt cgtggcgata tcgttcgccg cctgtaaggc cccggcctca
      121 tcaccggaac gctgcagctg cgcaacatac gcaatctgct ctgccgtcac gttacggaac
      181 tggcgcgcca tcgcaatcag ccccgacgta gggtcggtgg tcagttttcc gaaagcctct
      241 gcaaccttgt ccacctccac accggatgca gaagcaaaac gcgcgacact ctggttgatg
      301 gcatcaaact gttcaccacc acgcacaccg gcattcacca gggctgccag tgactcactc
      361 gcctggttaa acgtcagccc tgcggcctgt ccggctctgg agagcgtcaa catgcgatcg
      421 gcagtcagtc cggactgatt accggaaaga accagggttt tattaaacgc tgaaagcgtg
      481 gaatccccct ggtaccaggc gtacgccagc gcacctgtcg ccaccgccag cgaggtgacc
      541 ccgaccatcg gcagggtgat cgcaccggca agccccctga acatggggat catcccgccg
      601 aaggagtcct tcacctgacc gccctgttgc agcaggatca gccagggatt ctgaccaccg
      661 gcaagctgcg tggcgatatc cgtaaactgt gcgggcaggg ttcgcatggc cgctttatac
      721 tgcccgacgg aaatcccggc tttttgtgca gccagcgcct ggcggctcag gccctgttca
      781 acagcactgg cggtttttct ggcgtcggta tccagacctg aaaaatgacg ccttacccgg
      841 ctcatctgct catcgaaacg gaccgcatcc agactaaggt caataacaag atcaccaacc
      901 ggctgggaca tatctcacac ctccggaaat ccccgctgaa gccatcatta atgcggcatc
      961 atccaccatg acatccgcca catccgcaga cgataaaata tcgcgccctc cgtccccacc
     1021 gaaccggacg cctccggcaa gtcctgccgc tttctgcatc agcattttgt cctcatccgg
     1081 cctctccacc tgctcttcct catgccgggg gacaagcaga ctgaaatcag agggatgcat
     1141 atccggatcg caaaaaaaca ggctgagtac agcgtacgtc agcccggaaa aatgcatatc
     1201 cagctgggta tcctgaaaat aatgcgtgcg gtaaaaacgg tgccagtcgg catattcggt
     1261 ggatgtcatc ccggcaagca tggcgcgcca gtcgggtctc cccatctcac gcgccagtct
     1321 gagggcaaa
//
LOCUS       DAZGJW010000454         1307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZGJW010000454 DAZGJW010000000
VERSION     DAZGJW010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.458"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            92..292
                     /locus_tag="ACHKIK_005578"
     CDS             92..292
                     /locus_tag="ACHKIK_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO9086483.1"
                     /translation="MSSKNRPRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            complement(424..750)
                     /locus_tag="ACHKIK_005579"
     CDS             complement(424..750)
                     /locus_tag="ACHKIK_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO9086484.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVRFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(1089..1250)
                     /locus_tag="ACHKIK_005580"
     CDS             complement(1089..1250)
                     /locus_tag="ACHKIK_005580"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFO9086485.1"
                     /translation="MREATGTSGVDNATGPRLADTAERDYFILRERLMTMQKQLEGAQ
                     EYIRTQCLK"
     gene            complement(1214..1303)
                     /locus_tag="ACHKIK_005581"
                     /pseudo
     CDS             complement(1214..1303)
                     /locus_tag="ACHKIK_005581"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap.; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
BASE COUNT          359 a          352 c          286 g          310 t
ORIGIN      
        1 cgggtgttca gagttttgat tctgcccggt aacgtacgaa gcacagccat gtaaaatcct
       61 cgccatatag cttgtcacca gaggaaagaa aatgtcatcg aaaaaccggc cccgcagaac
      121 aacaacccgc aacatccgtt tccccaatca catgattgaa cagatcaata tcgcccttga
      181 acataaaggt tccggtaact tttcagcgtg ggttattgaa gcctgcagga gaaggctggc
      241 aacagatgca acgcatctgc gcccggccag catgacaaat aacgagaaat gaacgttcgg
      301 ttacaggagc aggtacccac tgtcctccca caatatttca tcttcatatc cgacggaaca
      361 agacttaccc tgccgggatg tacagaataa caacagagtg ataattaatt tctgatgaaa
      421 taatcagggt gcagaaggac taaagataaa cgttttcttc acgcctttac acggcctgtc
      481 cttctcaaat cgccattttg ccatcgcctt tacaacctgc tcatcaaaca gatggtgcgg
      541 ctctgaacga ataaactcaa ttcgggtgac agtaccatca gcaccaatat caaacctcac
      601 atcaacccgt ccctttatat aatttgccgc tgcataggcc ggatattgtg gtaatgcctt
      661 aaccaactgt cggggcatat ctgttttatg ttgcgtacag cccataacca gagaagacaa
      721 caaaataatt aacggaagat ttcttttcat tttcattccc cgcacagata aaaataagtc
      781 ttattctaac aatgccgccc tgtcggtcat caatcctctg cttaatggca acgacaatta
      841 tccgacttaa atcacaaatc agacacatga cataacaggg cttgcgaggt aacacatcgt
      901 ccggtttctt ccaccatcgc accggactgg cgactatgag gggacaacgc cgcgctccgt
      961 taacgcggta aaccccggtg tgtatcgttt ttgattatcc ccgcacactc gcgcagagga
     1021 gtctccctgt cgggctgcgg tctctgttaa tgagggaata cagcgacgat acggcgcatc
     1081 aacaaaactt atttcaggca ctgagtacgg atatattcct gcgccccttc cagctgcttc
     1141 tgcattgtca tcaaccgttc tctgaggatg aaataatccc gttcagcggt gtctgccagt
     1201 cgggggccgg ttgcattatc cacgccggag gtgccggtgg cttcacgcac ggtaccggag
     1261 caggtggcgt tgatccgcag gcgcttacga ccagcggcaa catcagc
//
LOCUS       DAZGJW010000455         1306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZGJW010000455 DAZGJW010000000
VERSION     DAZGJW010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.459"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            205..1287
                     /gene="ompC"
                     /locus_tag="ACHKIK_005582"
     CDS             205..1287
                     /gene="ompC"
                     /locus_tag="ACHKIK_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO9086486.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYTRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTQANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGKNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
BASE COUNT          402 a          257 c          274 g          373 t
ORIGIN      
        1 taaattggct aatagattta tttttattca gccaagagcc atgaatagga ttcgatagaa
       61 aaagttcaga taaaaataga gatctacttc acaaatcaaa tgagaaacca aaacttacat
      121 cttgaaataa tcacattgat tagatgaata tttatcgcgc agtgacatca ttttttaata
      181 atagttcaaa aaaaagggct cacgatgaaa aaattaacag tggcaatttc tgctgtagct
      241 gcatcagtac tgatggcgat gtctgctcag gcagctgaaa tttataataa agacagtaac
      301 aagctggatc tgtacgggaa agttaatgct aagcactact tctcctctaa tgatgcagat
      361 gatggtgata ctacttatac ccgtcttggc ttcaaaggtg aaacccaaat caacgatcaa
      421 ctgactggtt tcggtcagtg ggaatatgaa ttcaaaggca accgcgctga atctcaaggt
      481 tcctccaaag ataaaacccg tcttgccttc gctggcctga aattcggtga ctacggctcc
      541 atcgattatg gccgtaacta cggtgtagca tacgatattg gtgcatggac cgacgttctg
      601 ccagaattcg gtggcgatac ctggacccaa acagatgtat tcatgactgg tcgcaccaca
      661 ggtgttgcaa cttatcgtaa caatgacttc tttggtctgg ttgatggtct gaactttgct
      721 gctcagtatc agggtaaaaa tgaccgtact gacgtaactc aagctaatgg tgatggtttc
      781 ggtttctcca ctacttatga gtatgaaggt ttcggcgtag gtgcaaccta tgctaaatct
      841 gatcgcacta ataatcaggt tatctacggt aacaacagcc tgaatgcatc tggtcaaaat
      901 gctgaagtat gggcagctgg tctgaaatat gatgcgaaca acatctatct ggctactacc
      961 tattctgaaa cccaaaacat gactgttttt ggtaaaaacc atattgccaa caaagcacaa
     1021 aacttcgaag tagttgcaca atatcagttc gacttcggtc tgcgtccgtc cgttgcttat
     1081 ctgcaatcta aagggaaaga cttgggtgct tggggtgatc aggacctggt tgaatatatt
     1141 gatgtaggtg caacctatta cttcaacaaa aatatgtcca cttttgttga ctacaaaatc
     1201 aacctgattg ataagagcga tttcacgaaa gcatctggcg ttgctaccga tgatatcgtt
     1261 gctgtaggta tggtttacca gttctaattt gattactaaa acatat
//
LOCUS       DAZGJW010000456         1306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZGJW010000456 DAZGJW010000000
VERSION     DAZGJW010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.460"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            62..>1306
                     /locus_tag="ACHKIK_005583"
     CDS             62..>1306
                     /locus_tag="ACHKIK_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO9086487.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFDGTPGNHAAQFGALVDKFRADLTD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESSYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAIL
                     RHAGRVALNAGASSTVSEVRPSS"
BASE COUNT          306 a          312 c          407 g          281 t
ORIGIN      
        1 tttttatgtg ttacatgatt gtgtattccg gataattact ttgctggcgg caggagaggt
       61 tatgacattt aaacactacg atgtggtcag ggcggcatcg ccgtcagacc ttgctgaacg
      121 actgacacaa aaactgaagg agggctggca gccatttggc agtccggtgg ccatcacgcc
      181 ttataccctg atgcaggcca ttgcggcgga aggtgatgtc accacaccag tggcggtgac
      241 cggtaatgag ggtaaggcgg tggctgtcag tgccaccagc gacccggagt attactttgt
      301 tgtggttctg gcagggcagt caaatggcat gtcgtatggt gaaggtcttc cgctgccgga
      361 gacatatgac cgtccggacc cgcgtattaa gcagctggcg cgccgcagta cggtgacacc
      421 gggcggtgca gcatgcaaat ataacgacat cattccggcg gaccattgtc tgcatgatgt
      481 gcaggacatg agccgtctta accatccgaa agcggacctg tcaaaagggc agtacggtac
      541 cgtggggcag gggctgcata tcgccaaaaa actgctgccg tttataccgg cgaatgcggg
      601 cattctgctg gttccgtgct gtcgtggtgg ttcagcgttc accaccggag ctgatggcac
      661 atacagtgac gcgagtggtg cctcggagaa ttcaacccgc tggggtgtgg acaagccgct
      721 gtataaggac cttatcggtc gaacaaaagc agcactgaag aagaacccga aaaatgtgct
      781 gtttgccgtg gtgtggatgc agggggaatt tgattttgac ggcacgcccg gaaatcacgc
      841 agcacagttt ggtgcgctgg ttgataaatt ccgtgcagac ctgacggata tggcaggtca
      901 gtgcgttggt ggctctgctg gcggtgttcc ctggatctgt ggagatacga cgtatttctg
      961 gaagcagaag aacgaatcct cgtaccagac ggtgtacggc agctacaaaa acaaaacgga
     1021 aaagaatatc catttcgtac cgttcatgac cgatgagaac ggggtgaatg tgccgacgaa
     1081 caaaccggaa gaagacccgg acattccggg tatcgggtat tacggttcga aatggcgtga
     1141 cagctcagcc acctggacgt cacaggacag ggcgagccat ttcagttcat gggctcgccg
     1201 cgggattatt tccgaccgtc tggcaacggc gattttgcgc catgcgggaa gagtggcgct
     1261 aaacgcgggg gcatcatcga cagtatcaga ggtgcgcccg tcatcg
//
LOCUS       DAZGJW010000457         1296 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZGJW010000457 DAZGJW010000000
VERSION     DAZGJW010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.461"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACHKIK_005584"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACHKIK_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO9086488.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          383 a          242 c          265 g          406 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgtt
//
LOCUS       DAZGJW010000458         1294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZGJW010000458 DAZGJW010000000
VERSION     DAZGJW010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.462"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            28..432
                     /gene="stpA"
                     /locus_tag="ACHKIK_005585"
     CDS             28..432
                     /gene="stpA"
                     /locus_tag="ACHKIK_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO9086489.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(479..1003)
                     /gene="ygaP"
                     /locus_tag="ACHKIK_005586"
     CDS             complement(479..1003)
                     /gene="ygaP"
                     /locus_tag="ACHKIK_005586"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO9086490.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(1013..>1294)
                     /locus_tag="ACHKIK_005587"
     CDS             complement(1013..>1294)
                     /locus_tag="ACHKIK_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO9086491.1"
                     /translation="LQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGELTRITGL
                     SASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          341 a          313 c          314 g          326 t
ORIGIN      
        1 ttttggcgtt aaaaggtttt ctttattatg tccgtaatgt tacaaagttt aaataacatt
       61 cgcactctcc gtgcgatggc tcgcgaattc tccattgacg ttcttgaaga aatgctcgaa
      121 aaattcaggg ttgtcactaa agaaagacgt gaagaagaag aacagcagca gcgtgaactg
      181 gctgagcgtc aggaaaaaat tagcacctgg ctggagctga tgaaggctga cggaattaac
      241 ccggaagagt tattaggtaa tagctctgct gctgcaccac gcgctggtaa aaaacgccag
      301 ccgcgtccgg cgaaatataa attcaccgat gttaacggtg aaactaaaac ctggaccggt
      361 cagggccgta caccgaagcc aattgctcag gcgctggcag aaggtaaatc tctcgacgat
      421 ttcctgatct aattactgag gccggatgtc gctgctaggg cgcgtccggc gtcgcttctc
      481 aagttcgttg gttccacggc atcttatcta acaaccttgc cattccacaa aaaccgctga
      541 ttcctgcaaa cagtaacccg gcaccaacaa agccacttaa taagaagaaa ccgctattta
      601 cggtataacc cagtacaacg ccgattaata ttaaaccacc cgcagcgatc tgcacctggc
      661 gcattaacgg caagggttga gatttattta ccgctactgg caatccagct cttttccagc
      721 catcaatccc atcttcgagt aaaaatattt ctgcgggggc ggcaatcgct gctaatttat
      781 cggcgttatt actggtgcgt ttacctgcct ggcagtggaa tataatttgc tcgcgacgta
      841 atttaggtgg aagacctgac tgttccagca cggataatgg agccagatct gcttcaggaa
      901 tatgttcacg aagatattca tcagcgtcac gaatatcgat taactttgcg ccgcgtgcga
      961 ttaattcttg tgcatcatgc ggcgaaatgg ttgtcaaagc catagtgact ccttacggac
     1021 aatagacatt tttcagggtg gcgataatgg catttaccgc ctcattttta atggaatata
     1081 gaatgcgttg ggcatcccgt tggctgtcga taagcccttc gtcccgcata cgagcgagat
     1141 gctgtgatgt cgcagaggca ctcagtccgg taatgcgcgt cagctctccc gcgctggtgc
     1201 cgggggaacc gctaagcatg cacagaatca gcaatcgttt aggatggctc attgctttca
     1261 ataaggccgc tgcctgttcg gcactggcct gtaa
//
LOCUS       DAZGJW010000459         1287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZGJW010000459 DAZGJW010000000
VERSION     DAZGJW010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.463"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(432..650)
                     /gene="ydfC"
                     /locus_tag="ACHKIK_005588"
     CDS             complement(432..650)
                     /gene="ydfC"
                     /locus_tag="ACHKIK_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO9086492.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(722..1021)
                     /locus_tag="ACHKIK_005589"
     CDS             complement(722..1021)
                     /locus_tag="ACHKIK_005589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086493.1"
                     /translation="MALYIDISAIAGQVRIIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IKLRGLIFSYKVTTIFADGSRETVRAMRFKGCVKDFATTFWARKLDCIHNQFPLE"
BASE COUNT          300 a          337 c          302 g          348 t
ORIGIN      
        1 agccgcattc accgcatcac aaaattcact ttaaaaaggg cggcagagca gccacggagt
       61 aaaactgata ccgccaaacg tcaccagaaa attgataaca gagggcgttg cagcggggtt
      121 gtcacttaag cgtatggtca acctgacaac ccggtgtcct caactgggga aggaataacc
      181 ccgccatact taccgccgcg ccatttcgcg gattgccaca accggaagcg cacggtcgac
      241 gaaaatttaa cgacaggcta tctatgaacc agctacctcg ccgtgcgctt tcgcgttatg
      301 gtctgacttt tcagggaaat atcctttcag taaactgtca gtgccggatg ctcacccgtg
      361 tccggcgcac gcactccacc tcacccgtgg agaactcctt aattaccaac cttagcttcg
      421 ttggttagct attaacgcgg gtatgtaatc attctggcaa tgcttaatgc cgctgctttt
      481 tccagattgg tgatatcctg ctccagagcg gacagatttt cagcctgctt agccctggct
      541 tcattggccc atttcaggtc ctgcgctgcc ttaattttct ggtgcatcca ctcataaagt
      601 tcatcatcgg tatagtctgg cgcgatgatg acgggttctc gtttctgcat gtcggctcct
      661 tgtggttagc gctgcctgct tttaaccacg tcaggcgagg tggtatcctc tgaggggtct
      721 gttactcgag aggaaattgg ttatgaatac aatcaagttt tcttgcccag aatgtggtgg
      781 cgaagtcttt gacacatcct ttaaaccgca tggctctgac agtttcgcgg gagccatctg
      841 caaaaattgt ggtcaccttg taactgaaga tgagtcctcg cagtttgatg acgaaatcgt
      901 tgacaatatc ttcggtgcac tcaccagaga ctttctgaag taaaggcgca taccgcttag
      961 ttaccgctct gataattctt acctgtccgg caatggcgct gatatcaata taaagcgcca
     1021 tcgctgtttc tttgctgatc cctggatgcc ttccattctg atgtttgact tcgcccactg
     1081 agaaatcctc tgtttcccct taacgccggg gtagcggaac aaaaaacctg ctgcatagtt
     1141 aaacgtaatc cccgccgtca tgttcatacg cctcaggctg gctacttaac ccctgaccac
     1201 tgccgggtaa ctcgaagtat tgccctgcgt tctgtggggc ggggtgggtt tatggataca
     1261 atttacaaat taaaatttaa ctggtca
//
LOCUS       DAZGJW010000460         1283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZGJW010000460 DAZGJW010000000
VERSION     DAZGJW010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.464"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..785)
                     /locus_tag="ACHKIK_005590"
     CDS             complement(<1..785)
                     /locus_tag="ACHKIK_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086494.1"
                     /translation="MLKSFCLNFDGMYLSIVSFLSEKFNLKRVGKMELESKEDYEETT
                     HHNVASSVVTATHDNLDGREFFELCSNPALVFSFETHKKFNELPVDSKESPLDLFIAN
                     TKQLHLLRSGDISNTLSHLLLLGYVSAAESYLRALIRNLINHDEYIQSIVSDKAVSYA
                     AAQHHKKELLPEALLEDFSLASPYNVFETLKDIIGMKGQRPIEMMKCSSEFMKVCELR
                     HCCVHRFGKLGSKNAIRLGLAEHMKHLEKPIILNNDDLEQIAF"
     gene            complement(763..>1283)
                     /locus_tag="ACHKIK_005591"
     CDS             complement(763..>1283)
                     /locus_tag="ACHKIK_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO9086495.1"
                     /translation="ISHGDGRTYERYIPHYALLIKERVFNEGSRGFSPSQIIQKILRK
                     NAKHPVRRNISSLFNAEQLARLKLTLPPCNNCKTPRLTDNQKFCHHCGNQLIDESAYN
                     QCMSIPLSKVPHLTSWQMQKLSGLEKVKTIGDVLSLQDPGSELRKIHRIGPKIANKIM
                     DKVLLHVEEFLS"
BASE COUNT          392 a          244 c          208 g          439 t
ORIGIN      
        1 ataaatgcga tttgctcgag atcatcatta ttgagtatta tcggtttttc taagtgcttc
       61 atatgttcag ccaatcctag tcggattgca tttttcgaac ccaacttacc aaatcgatga
      121 acacaacaat gccttaactc gcaaactttc atgaattcgc ttgagcattt catcatttcg
      181 attggtcgtt gtcctttcat tccaataatg tcctttaatg tttcaaaaac attataaggg
      241 cttgccaatg aaaagtcctc aagtaaagct tctggtaata actccttttt atggtgttgt
      301 gccgctgcat aactaacggc cttatcagaa actatagact gtatatattc atcatgatta
      361 attagatttc tgataagagc tcgcaaataa ctttcggctg cagacacata acctaataac
      421 aacagatggc ttaatgtgtt tgaaatatca ccagatctta acaagtgaag ttgctttgta
      481 ttagctataa aaagatctaa cggggattcc ttgctatcaa caggtaactc attaaatttt
      541 ttgtgtgtct caaacgaaaa aactagagct ggattactac atagttcaaa aaactctctt
      601 ccatctaagt tatcatgggt tgcagtgaca actgaggacg ccacattatg atgagtggtc
      661 tcttcataat cctcttttga ttctagttcc attttcccga ctctttttaa attaaacttt
      721 tcagataaga aagacacgat tgacaagtac atgccatcaa aattaagaca aaaactcttc
      781 aacatgtaat aacactttgt ccataatttt attggcaatt ttgggtccta ttctgtgaat
      841 ttttcttaac tcactcccag ggtcttgtaa agacaataca tctcctatag tttttacttt
      901 ttccagccca gatagttttt gcatttgcca agatgttaga tgtggtactt ttgacagtgg
      961 aatagacatg cactgattat aggccgactc atcaatcaat tgattcccac aatgatggca
     1021 aaatttttga ttgtcagtca atcgcggagt cttgcagtta ttgcatggag gtaaagttag
     1081 tttaagtctc gcaagttgct cagcattgaa taatgatgaa atgtttcttc tgacaggatg
     1141 cttagcattc tttcttagta ttttttgaat aatctgagaa ggagaaaaac ctctacttcc
     1201 ttcgttaaac actctttcct ttattaataa agcataatga ggtatatatc tttcataagt
     1261 acgcccatca ccatgagaaa tat
//
LOCUS       DAZGJW010000461         1278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZGJW010000461 DAZGJW010000000
VERSION     DAZGJW010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.465"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1014
                     /locus_tag="ACHKIK_005592"
     CDS             <1..1014
                     /locus_tag="ACHKIK_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9086496.1"
                     /translation="GFNVGSPQQAGISELVLRAGNGNPKGITGALWQRTSTGFTNFAW
                     VNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPEGLTDGTVYSLY
                     TPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIK
                     GKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSG
                     STSSAGAHSHVDGRRFNTSTFKDTYQYGSTAVGSNSYQVQGAVGLGIGTMANTNSAGA
                     HTHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIV
                     RLA"
     gene            1014..>1278
                     /locus_tag="ACHKIK_005593"
     CDS             1014..>1278
                     /locus_tag="ACHKIK_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO9086497.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFIS"
BASE COUNT          330 a          311 c          320 g          317 t
ORIGIN      
        1 ggatttaacg tggggtcacc tcaacaggca ggtatatctg aacttgtttt gcgtgcaggt
       61 aatggtaatc cgaaggggat tactggtgct ttatggcagc gcacatcgac agggtttaca
      121 aattttgcct gggtcaatac atctggtgat acttacgata tttacgttgc aatcggaaat
      181 tatgcgactg gtgtaaatat tcaatgggat tataccagta atgccagcgt gacgattcat
      241 acgtcaccag catattctgc taataagccg gaagggttaa cggacggtac agtttattca
      301 ctctatacgc catcagagca gttttatccg cctggcgcac caatcccgtg gccatcagat
      361 accgttccgt ctggctatgc cctgatgcag gggcagactt ttgacaaatc tgcatacccg
      421 aaacttgcag ccgcttatcc gtcaggcgtg atccctgata tgcgtggctg gacgattaag
      481 ggcaaacccg ccagtggtcg tgccgtattg tctcaggaac aggacggcat taaatcgcac
      541 acccacagcg ccagcgcatc cagtacggat ttggggacga aaaccacatc gtcgtttgat
      601 tacggcacta aatccacgaa taacaccggg gctcatacgc acagtctgag cggctctaca
      661 agctcggccg gagcacactc gcacgttgat ggtcgcaggt ttaatacaag cacttttaaa
      721 gacacctatc agtatggttc tacagctgtc ggaagtaact cataccaggt acaaggggcg
      781 gttggtctgg gcattggcac gatggcgaat acgaactctg ctggcgcgca tacccactca
      841 ctgtccggca ctgctgcaag cgcaggtgca cacgcacata ctgtcggtat tggtgctcat
      901 acgcactccg ttgcgattgg ctcacatgga cacaccatca ccgttaacgc tgctggtaac
      961 gcggaaaaca ccgtcaaaaa catcgcattt aactatattg tgaggcttgc ataatggcat
     1021 tcagaatgag tgaacaagca cggaccataa aaatttataa tctgctggcc ggaactaatg
     1081 aatttattgg tgaaggtgat gcatatattc cgcctcatac aggtctgcca gcaaacagta
     1141 ccgatattgc accaccagat attcctgctg gctttgtggc tgttttcaac agtgatgagg
     1201 catcgtggca tctcgttgaa gaccatcggg gtaaaacggt ttatgacgta gcgtcagggg
     1261 acgcgttatt tatttctg
//
LOCUS       DAZGJW010000462         1269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZGJW010000462 DAZGJW010000000
VERSION     DAZGJW010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.466"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..480
                     /locus_tag="ACHKIK_005594"
     CDS             <1..480
                     /locus_tag="ACHKIK_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EspJ family T3SS effector
                     ADP-ribosyltransferase"
                     /protein_id="HFO9086498.1"
                     /translation="NYITEKVLSIFNIKRDFVAVRIQSNQFADLKNKTIQGHKDTVAK
                     VIDWYNPQKNAFGIMMGTPRRSADIAKEESRNALNFMIMEKDTFNEKILNSNANLQKK
                     YGTTENSSWVSASVGSLLDKGAKVYPDISCSLRLGEPFIITLPETVRLDVNIHPLKK"
     gene            complement(493..714)
                     /locus_tag="ACHKIK_005595"
     CDS             complement(493..714)
                     /locus_tag="ACHKIK_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086499.1"
                     /translation="MDIQCTLANTFPNEHPEAFILVTSAEKFIVDGNCIQLIGIGNEQ
                     GEFDFYRQIYPVTGGEHKVEALMWTHLPA"
     gene            complement(798..>1269)
                     /locus_tag="ACHKIK_005596"
     CDS             complement(798..>1269)
                     /locus_tag="ACHKIK_005596"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086500.1"
                     /translation="AGEKTIKVSSIISQNTGHFLVPHRSELRCIMNEIRSNPVKFLEE
                     HLILNTQRYAHHDETSLITVNITREDGLLRLKETEYDMAGSDYILFTAMRDTDSPERF
                     DAPAVVSLSNQCISLRRNDISQTIQQSSPLWLTNQQSGCSVLIVRHGLSESGEQ"
BASE COUNT          421 a          270 c          217 g          361 t
ORIGIN      
        1 aactacatta cagaaaaagt tttatctatt ttcaatataa aacgggattt cgttgctgta
       61 agaatccaaa gtaatcagtt tgctgattta aaaaacaaga caattcaggg tcataaagac
      121 actgtcgcaa aagtcataga ctggtataac ccacaaaaaa acgcatttgg tatcatgatg
      181 ggcacaccaa gacgttctgc tgatatagca aaggaagaat ccagaaacgc tctaaatttc
      241 atgataatgg aaaaggatac atttaatgaa aagatactta acagcaatgc taatttacaa
      301 aaaaaatacg gcacaacaga aaatagtagt tgggtatcgg cttctgttgg ttctcttctg
      361 gacaaaggag ccaaagtata tccagatata tcatgctctc tgagactggg agaaccattc
      421 attattacac ttccagaaac agtcaggctg gatgttaata tccacccact caaaaaatga
      481 tcctgaacgt ttttatgcag gcaaatgcgt ccacatcaag gcttcaacct tgtgctctcc
      541 ccctgtcaca ggataaattt gcctgtaaaa atcaaactct ccctgctcat taccaatacc
      601 aatcagttga atgcagttgc catctacaat aaatttttcc gctgacgtaa ccaaaataaa
      661 ggcttccgga tgttcattag gaaaggtgtt agcaagagta cactgaatat ccatctccaa
      721 caaaatctcc tgattattag cgtaaactca tcgacgaaat cgttactatc ccctgggcac
      781 atatgtacca ttgaatacta ctgttctcca gattctgata atccatggcg aacaataaga
      841 acactacagc cgctttgctg attagttagc cataacggtg aactttgctg gatagtttga
      901 gaaatatcat ttcgccggag gcttatacac tgattactca agcttacaac tgcaggtgca
      961 tcaaagcgct ccggactatc agtatcacgc atagctgtaa acagaatata atcactacca
     1021 gccatatcat attccgtttc ttttagtctt aacaagccat cctctcttgt aatattaact
     1081 gtaattaaag atgtctcatc atgatgggca tagcgctgtg tattaagaat taaatgctct
     1141 tccagaaatt ttacagggtt actacgtatt tcattcatta tacaccgaag ctctgaacga
     1201 tgcggcacca gaaagtgccc ggtattttgt gaaataatac tacttacctt gattgttttc
     1261 tctccagct
//
LOCUS       DAZGJW010000463         1265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZGJW010000463 DAZGJW010000000
VERSION     DAZGJW010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.467"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>1265)
                     /locus_tag="ACHKIK_005597"
     CDS             complement(<1..>1265)
                     /locus_tag="ACHKIK_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9086501.1"
                     /translation="IPPAVQHLTVEVSAADGQYLAQAKWDTPKVVKGVSFMLRLTVVA
                     DDGSERLVSTARTTETTYRFTQLAPGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPS
                     QIELTPGYFQITAVPRLAVYDPTVQFEFWFSETRITDIRQVETTARYLGTGLYWIAAS
                     INIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQ
                     IDNGQLAPDLAEIRTSITDVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWA
                     VKLQQMKDGRLYIAGIGAGIENTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQ
                     IFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINE
                     NCQIKGKLSANQIEGDIVKTVGKAFPRDSR"
BASE COUNT          259 a          381 c          305 g          320 t
ORIGIN      
        1 cgggagtccc gcggaaaagc cttacccact gttttgacta tatcgccttc aatctggttg
       61 gctgacagtt tccccttaat ctgacagttc tcattaattg tgacattgtt gagcgtcccg
      121 gcgttcgcat tcacactgcc actgatatct gcatttttag cggtcagctt tccgtccggt
      181 gtcagggaaa atgccggagg attaccgccg ctggtaatgg tgggagccgt caggcgtttc
      241 aggaacacgt cgttcatgaa tatctgatcg ccctgaccaa caaacatcgg ctttgtgttg
      301 ccattcgcag gattaatcat cgcaatcctg tctgccgcca gcagcacctg actctgcatt
      361 cctgctggcg tattctcaat accggcaccg atacccgcaa tataaaggcg tccgtccttc
      421 atctgttgca gcttcacagc ccacatgctg ttcagattat tatttgtatc aacctgaacc
      481 ttctgtatct gctggatcgc tgcactctgg tcttccagtt tcttattgac ggtctgcgtg
      541 atttcattac tgacatccgt gatggacgtt ctgatttccg ccaggtcagg cgcaagctga
      601 ccgttatcaa tctgagtcca caactcctga gccagatggg ttttccctat ctctcctttg
      661 aaaaaatcca gatagccgga tgcatcatca ctcggctggc caacagcctc cacaaatgcc
      721 gatttgccaa cggtgttcac actgcggata taaaagtaat aatcatggcc cggtttgata
      781 ttgatactgg cggctatcca gtacagcccc gtgccaaggt agcgggctgt ggtttcaacc
      841 tgcctgatat cggtaatccg cgtttccgaa aaccagaact caaactgtac cgtcgggtca
      901 tacaccgcaa gacgcgggac cgctgttatc tgaaaatagc ccggtgtcag ctcaatctgt
      961 gacggcgctg ccggtgcggc aatccggaac gataccgacg ccggatcgcc ctgctgcccc
     1021 cacgcattta ccgcccggac tgtcagcctg tagttccccg gcgccagttg cgtgaagcgg
     1081 tatgtggttt ccgtcgtccg ggccgtgctg accagccgct cactgccgtc atccgcgacc
     1141 acggtcaggc gaagcataaa gctcacgccc ttcaccacct tcggcgtgtc ccatttcgcc
     1201 tgcgccagat actgaccgtc agctgcgctc acctccaccg tcaggtgctg cactgccgga
     1261 gggat
//
LOCUS       DAZGJW010000464         1258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZGJW010000464 DAZGJW010000000
VERSION     DAZGJW010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.468"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(240..956)
                     /locus_tag="ACHKIK_005598"
     CDS             complement(240..956)
                     /locus_tag="ACHKIK_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO9086502.1"
                     /translation="MSTIATSLGSNIGYMKNTISERIRNRRKDVGLTQQQVAKAIGIS
                     RVSVTKWENGSSKPDGENLYLLSKLLSKSPEWILYGKDGHDKTDDLRLNQYPYISDNI
                     ARLPVLTWEQAGYWDMSCPVTKIPGIKNWVDVMTKTAENSFLLHVEGDAMTNSNGLPT
                     IPDGSTVLITPCSSNIRELVGKIILIQLEGTPNVTLKKVAIDGPNIYLLSLNPLYKPI
                     ELNGGYTIKGKVSQIHQYLD"
     gene            1006..1221
                     /locus_tag="ACHKIK_005599"
     CDS             1006..1221
                     /locus_tag="ACHKIK_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO9086503.1"
                     /translation="MFKDDVLRYFKKKRLVAEALGISHVAVVRWKAVIPKLRAMELDE
                     ITNGELKYNPELYKKQDSTSNEGKNDS"
BASE COUNT          345 a          253 c          283 g          377 t
ORIGIN      
        1 ggaacaaaaa cctgctgcat agttattaaa gttgaaccct gccgtcatgt tcttacgcct
       61 cgggctggct acttaacccc tgaccactgc cgggtaactc gaagtatttc cctgcgttct
      121 gtggggcggg gtgggttggt atgttgttaa ggtaacaaga gttacctttc gagtcaatac
      181 aatgttgcaa aaggtacatt tgagggcata aaaaacccgc aatgaatgcg ggttctgact
      241 cagtctaagt attgatgtat ttgtgaaact ttacctttaa tggtgtaacc accattcagt
      301 tcgatgggtt tgtaaagcgg attcagtgac aacagataga tgtttggtcc gtcaatcgca
      361 acttttttta gtgttacgtt tggcgttcct tccaattgga ttaagattat ttttcccacc
      421 agttctctaa tgttacttga gcatggtgtg atcagcacgg tagatccgtc ggggatggtt
      481 gggaggccgt tagagtttgt catcgcatct ccctcaacat gcaataaaaa agagttttca
      541 gcggtttttg tcatgacatc aacccagttc ttaataccag gaatcttggt tactggacaa
      601 ctcatatccc aataaccagc ctgttcccac gttaaaacgg gcaaccgggc gatgttgtca
      661 ctaatgtaag ggtactgatt cagacgcaga tcatcggttt tatcgtgacc gtcctttcca
      721 taaagaatcc attcaggaga tttggaaagc aattttgaca gtagatacaa attctcaccg
      781 tcaggttttg aagagccatt ttcccatttt gttacggata cacgagatat gccgattgct
      841 ttcgcaacct gctgttgggt taatccaacg tcttttcgac gattccgaat acgttcgctg
      901 atagtgtttt tcatgtaacc aatgttacta ccaagtgatg ttgctatggt tgacattgtt
      961 atgtaactat tgttaccctt ctgctcgaaa taacaggaga gttttatgtt caaagatgat
     1021 gttctgcgct atttcaaaaa aaagcgacta gtagctgagg ctcttggaat ttcacatgtg
     1081 gctgttgtgc ggtggaaagc agttattccc aaacttcgcg caatggaact ggatgaaatt
     1141 actaacggtg aattgaaata caacccagaa ctttacaaga agcaggatag cacctcgaac
     1201 gaaggaaaga atgattcatg aaaatcaagc atgaacacat ccgcatggcg atgaatgt
//
LOCUS       DAZGJW010000465         1253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZGJW010000465 DAZGJW010000000
VERSION     DAZGJW010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.469"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..375
                     /locus_tag="ACHKIK_005600"
     CDS             <1..375
                     /locus_tag="ACHKIK_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9086504.1"
                     /translation="GAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVERNIKVPL
                     TEPQKAGIASFCPYNIGPGKCFPSTFYKRLNAGDRRGACEAIRWWIKDGGRDCRIRSN
                     NCYGQVSRRDQESALACWGIDR"
     gene            650..1219
                     /locus_tag="ACHKIK_005601"
     CDS             650..1219
                     /locus_tag="ACHKIK_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO9086505.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRGKVIQYQEEC
                     DDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKSAHVS
                     KIRTLINEANLLIDFVLADTDKGKITKAD"
BASE COUNT          353 a          257 c          346 g          297 t
ORIGIN      
        1 ggcgccatca tggtggatgg taagcctgtg attcctggca tgaagctgtc gaaggaaaaa
       61 tgcgaccggg ttaacgctat cgaacgggat aaggcgctgg catgggtgga gcgtaatata
      121 aaagttccac tgaccgagcc acaaaaagca ggtatcgcgt cattttgccc ctataacatt
      181 ggccccggta agtgtttccc gtcgacgttt tataagcggc ttaatgcagg cgatcgcagg
      241 ggagcgtgtg aggcgattcg ctggtggatt aaggacggtg gcagagactg ccgtatccgt
      301 tcaaacaact gttacggtca ggtatcaaga cgtgaccagg agagtgcgct ggcatgctgg
      361 ggtatcgaca ggtaagcaga atattttgct gaaaaatgac gttggccaac gcgggtagac
      421 aacacgaaat cctgcgaact ggcaaaatgt aagtgaataa agtcaacaag attgtttcat
      481 gaagaggcac cgtaatggtg cctttgtcat ttctgcgctt cgcacaagcg taaataaacc
      541 aaagaacctt tcaggatgag ccctggtgga taaccggcag tggtctggtt aaccctcttt
      601 gggctggtta ttcctgtgcg cagggttcat cactaaaagg aaataaccga tgaatatgat
      661 gaccgtgccg tttcacggcg attctcttta tgtggttaac cataacggcg aaccatacgt
      721 tcccatgaaa cctgtcgttg cggggatggg gctggcctgg caatcacagt tggctaagtt
      781 aagacagcgt tttgcgtcaa ctataacgga aatcgttatg gttgctgagg atgggaaacg
      841 acgcaatatg gtgtccctgc cgcttcgaaa acttgcaggc tggttacaaa ccatcaatcc
      901 caacaaagta aaacccgaaa tccgcggcaa ggtaatccag tatcaggaag agtgtgacga
      961 tgttctctat gaatactgga cgaagggttt tgtcgttaat ccccgtcgaa tgagtgtgat
     1021 ggaagaactc aatcaggctt gcgctgacat gaaacgggat aaaaacattg ccagtgtgtt
     1081 tgctaccggg ctgaatgagt ggaaacaggt taaatccgcg catgtatcaa aaatccgcac
     1141 attgataaac gaagcgaatc tgctgattga ttttgtcctg gctgatacag acaaagggaa
     1201 aataacaaag gcggattgat ggagtggtgg ctaatgatat cggataaact cat
//
LOCUS       DAZGJW010000466         1240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZGJW010000466 DAZGJW010000000
VERSION     DAZGJW010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.470"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            39..848
                     /locus_tag="ACHKIK_005602"
     CDS             39..848
                     /locus_tag="ACHKIK_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9086506.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
     gene            841..>1240
                     /gene="yqeJ"
                     /locus_tag="ACHKIK_005603"
     CDS             841..>1240
                     /gene="yqeJ"
                     /locus_tag="ACHKIK_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFO9086507.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKS"
BASE COUNT          424 a          224 c          234 g          358 t
ORIGIN      
        1 gtgatctcca tttcgattga tttagtgttt attgacgtat gtactggatt attaacgata
       61 atatcgagtt ctggcctgag caccgaaaat taatatcggt acataacgcc gatcttaacg
      121 tcgttctgac aacgccagcc agtcgatgtt tatcacttct acttgaagct tttcctgatg
      181 tggttgcaca acaagatttt ttcaccagag tctgggaaga agaaggtatg cgtgtgccta
      241 ctaacacgtt atatcagaac atatccatta tcagacgcgg atttcgcgct gttggtgata
      301 ctacccactc gctaattgca accgtgccga gaagaggatt caagatccat aatgacatta
      361 acatacaaaa tcatgtaata aactcgtcaa cagacgcaca tacacacaat gccccacctg
      421 ccataaaagt taatgcggga tacaaagaga gcattggtgg cgcaaagaat ttcaataaca
      481 aaatcctcaa acatataaaa agtcatctaa ttatgttgag cgcatttgtc ataggcgcat
      541 attctgcata ttggctatgg aataataatc aaccgaagcc atttttcaag gattacaaga
      601 ctgttgcgga aattaatggc tgccatttta atgtaacaga agatacaatt gacgggttga
      661 aagagttcga taaatataaa acacggatac tggattcggg gattaactgt aaaaaacatc
      721 cgtggttata cttccctctt gcaaaatcct caccggggat gattgttatg gcatgcaata
      781 aaaattataa ccaacatgaa gtggcagact gtttaaccct gtcttaccgt gaggttaatc
      841 gtgattgatt ataaaaaaaa cttattattt attttagttt ttatcagtgg gtttattctc
      901 ttcactgtct attcttacac tgcagaaaaa atgatatata acgaaacatg cactgcaaat
      961 tgggtaattt ttaatgacca gggacgagca aatcttacca tcgactttat gtataacaaa
     1021 aaaaataaaa caggaactgt tgcgttgagt ggaacctggc aacaaggtaa cagagaaagt
     1081 aaatcaatac ggcgaaatat tgaatacaca tggattgaaa actatgacac agcccattta
     1141 acatcaaaga aagttaataa gtttgagatt atggatcaag ttgatgatga tagacttgca
     1201 caacttattc ctgattttta tgtctttcca gaaaaaagtg
//
LOCUS       DAZGJW010000467         1220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZGJW010000467 DAZGJW010000000
VERSION     DAZGJW010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.471"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            693..1193
                     /locus_tag="ACHKIK_005604"
                     /pseudo
     CDS             693..1193
                     /locus_tag="ACHKIK_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535762.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT          358 a          234 c          255 g          373 t
ORIGIN      
        1 aataaagatt attgacaaaa ccaaatataa ataacgactg cctcggcatg ccagttaaca
       61 gctatccgta tggcgtgttc ggttttgcga ataaaaaatg gttactgtag aagcggattt
      121 ccaccacatg tcaaccatga atggtcagtg atgcgagacg actgggatga tttggtcgat
      181 gacttcttcc ggcgaatact gatagaaaca ttgaaatatt gtaagagaaa gcgttcaaaa
      241 ttctgtggca cgtgaaaaat tctacagcgt catcacctta ttcagtcgac cttcaaaata
      301 aacgtttaac agagacaatc accatttgga ttgtccgctg aggaggcgaa caatttccta
      361 gacagcttta tcatgtcagc ccacattgtt ggcgctggca atactaagga caaacgatgg
      421 ggaagtagct ttttagcgtt attttcggga gtaaaaaacg tggtatcttt ccgtatgtca
      481 gttgtatctt gctggtattc gtagtcgtga taaggcatac aacgatattt atcttgacgt
      541 tagtgaagaa taagttgttt tgtgatctat gttaatctgg gattacaatc cgggcttgat
      601 acatcgcatc acgactttta ttctcttcag aatagaatat gttactcccc gctttacagg
      661 ctcaggattt gaattacaaa ttatactttt aagtgaacaa taaccaaggg tatttcttat
      721 ttcctgaaac caggcttggc ccatttcggg atgagtttga aaaaactctt gacgcatacg
      781 atagtgatga aataaacgaa acaaattacg tcaacaaact caggcgtctt gcctgacatg
      841 ctcctgacat cattgatatt cacgctcatc tggcatatgc atttgcgctc tttatgctgc
      901 aattctggcg aatgttcatt tacagcgtca tcaggatgcc gttatactca ttgataaaat
      961 actaacatat aaccctgaga ataatcaggg aacacgctgg ttactggggg cggaattgtt
     1021 acgtgctggc aagcatggca gggctctgta cgtattgaca ggacatgtca atgaatttcc
     1081 cccctactgg tacgagcttg ctcttttaca ttttatgaat gaggcatata ttaatgctgc
     1141 aacggcattt cgatatggat ttttaacaag tatatattgt tgaaatgctt tgttagaatc
     1201 tttatccatt ccctagggct
//
LOCUS       DAZGJW010000468         1220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZGJW010000468 DAZGJW010000000
VERSION     DAZGJW010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.472"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..78)
                     /locus_tag="ACHKIK_005605"
     CDS             complement(<1..78)
                     /locus_tag="ACHKIK_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086508.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(114..539)
                     /gene="yqeK"
                     /locus_tag="ACHKIK_005606"
     CDS             complement(114..539)
                     /gene="yqeK"
                     /locus_tag="ACHKIK_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFO9086509.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKSSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
     gene            733..1220
                     /locus_tag="ACHKIK_005607"
                     /pseudo
     CDS             733..1220
                     /locus_tag="ACHKIK_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          447 a          222 c          175 g          376 t
ORIGIN      
        1 atcataaaac aataaaatta aatattctcg cagtatatgg cagtctaaag catcaaagtt
       61 ttgatcaaca tctttcattt tagacatctc cttgcaatgt aaaatatcat gtatcaacaa
      121 catctggtgc aaatccatga gtcgaactcg acattcatct tagctcgatt attattcctt
      181 cgtacagtcg atgtaaacaa tacagaaaga ggattattaa gaaccagttt actcagtgaa
      241 tcaatagagg aaaggactaa cgtttcttta aaagaattga tttcatcatc tgttaaacta
      301 aactcatcat tgacagatcg tgagatataa ctgcttttaa ctttactctt tacgttgact
      361 ttattgacag aattaacatt cacaaatctt gaatttaatg cccatgatgt tgtttgcgtg
      421 agaaccttct cagcattaag gaattttttt aacggaatcc ttggtttctt tttcgagtcc
      481 gaatttacaa tatcatgcat atagaccatt tcatgaatct gcgaaaactc aatgtccatt
      541 atatacctca catttttacc gtgactcgat gttactgttc aataatcacc ttccatcaat
      601 actaaaatta atacccctaa tgtgccgata acaaatatag tcattctacg taatgtctcc
      661 ataaggtgat atttgacatt atcagaagct gcgaattcgg attttgctct aatcaaatga
      721 gagaaatgta aaatgaagcc acgaaatatt aataatagcc taccactgca accattagtt
      781 cctgatcagg agaacaaaaa taagaaaaat gaagagaaat ccgttaatcc agttaaaatc
      841 acaatggggt ctggtttaaa ttatattgaa caagaatctc ttggaggaaa atatctaaca
      901 catgatttgt caataaagat agcggatatt tctgaagaga taattcagca agcaatatta
      961 tctgctatga gcatatataa attttcgata acagatgatt taatgagtat ggctgtaaat
     1021 gaactcataa aactgaccaa aatagagaat aatgtagacc tgaataaatt cactactata
     1081 tgcacagacg ttctatcccc ccgcgtcacc agacataata aagaaaaaaa acaaacgaca
     1141 ttctaccctt ctcaaaaatc ccctttttaa ttttcattga aaaaatggca attgatacta
     1201 tgaatccagg tcatacacat
//
LOCUS       DAZGJW010000469         1205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZGJW010000469 DAZGJW010000000
VERSION     DAZGJW010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.473"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..54)
                     /locus_tag="ACHKIK_005608"
                     /pseudo
     CDS             complement(1..54)
                     /locus_tag="ACHKIK_005608"
                     /inference="COORDINATES: protein motif:HMM:NF018510.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(39..782)
                     /locus_tag="ACHKIK_005609"
     CDS             complement(39..782)
                     /locus_tag="ACHKIK_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO9086510.1"
                     /translation="MTETESAILAHARRCAPAESCGFVISTPEGDRYQPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDACHLAGITLPDFHREDEWWRNGQNLYLDNMEAT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHVSAFTGIYNDLAAASACM"
     gene            complement(787..>1205)
                     /locus_tag="ACHKIK_005610"
     CDS             complement(787..>1205)
                     /locus_tag="ACHKIK_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9086511.1"
                     /translation="LQSLVGATVVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVE
                     QMSELTAMTASFVLATPTETDGALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNP
                     TTDIRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
BASE COUNT          255 a          397 c          319 g          234 t
ORIGIN      
        1 ctgcatcgac agggcacgga tggcttccgc tgccgtgttc acatacaggc tgaggcggcg
       61 gccaaatcgt tgtaaatccc cgtgaaggca gatacgtgcc agtggcggtg acgccagaca
      121 gaatgcgttc gtcgttgcca tttttcggaa tacctctccc gtttactcag ttgttcaggc
      181 agatggtgaa gcagttcacc gttgccgcag taaatggcag catggttggg taccgatgcg
      241 ccaaagcagc acagcaggat atcgcctgcc tgtgccgagg acaggggcac ccggtaaaaa
      301 ccagtggcct ccatattgtc cagatacagg ttctgaccat tgcgccacca ctcatcctcg
      361 cggtgaaaat ccggcagcgt tatccccgcc agatggcagg catccctgaa cagcgtgtaa
      421 cagtccgtca ccccgtgttc aaaacgacgc ccggtcaggt gcggcacaca gcggaattta
      481 tgaatgtccc cccggcagac cagccaccag ggcagggcac tttttatctg cagccgccgg
      541 tcggcctcgc tcagccaggg caggccaccg ggatggctgt ggaccagtgc cacaatctcc
      601 ccctgcatct ctgcccgcag ccagtcttcc ggggcaatac gaaaatatgc ctcaggctct
      661 gcagagatat tcacgcacgg ctggtaccgg tcaccctccg gcgtgcttat cacgaagccg
      721 caagactccg caggcgcaca ccgccgggca tgcgccagaa tcgctgattc tgtctctgtc
      781 atggatttac tgcgaaagtt tattaatgga aaggaaaccg ccaaaattag ccaccatgcc
      841 gcgcatctca cacccgcgca tgcacttgct gcatctgtcc ttacggatat ccgtggtggg
      901 gttgtcgaac tcatccgcca ccgcaggacc gttatacccg cattcatctc cccggtaatc
      961 ccacatacag gtgttcgcca gcatgatgcg accaggaaac agcgccccgt ccgtctccgt
     1021 cggtgttgcc agcacaaacg aggctgtcat ggccgtcagc tctgacatct gctccaccac
     1081 ccagcggtcg ctcagctcct gctccgggtc cgcttccgga ttgcccgcca caaaattcac
     1141 cgcatccaga aaacgggcat acacccggcg gcggaccacc gtggccccca ccaggctctg
     1201 caggt
//
LOCUS       DAZGJW010000470         1205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZGJW010000470 DAZGJW010000000
VERSION     DAZGJW010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.474"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(179..1054)
                     /locus_tag="ACHKIK_005611"
     CDS             complement(179..1054)
                     /locus_tag="ACHKIK_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFO9086512.1"
                     /translation="MFSIRTLLPISASVSVPTKQSQSIPITLAGRTIEKAQEKEGLLV
                     FLGMKSVNDYTLNILGQNVSRVTTGKKPYDLLFLNDATKQDFDKRKMEFTYPGANKSH
                     LQSSNSDVVAAAAISITATEMKTILPDDLTPGKYNKIYLSGHGSAGLPLLKCGDEFLS
                     PSDIVDRIVQHNLHEIDDIRLTSCNSANIIKNKDFSPDEIEKSANMNNGWLARALFGQ
                     KRSLAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHLRSTTVPATPEHTVRRS
                     DYRATLGRTQPIDIE"
BASE COUNT          324 a          247 c          237 g          397 t
ORIGIN      
        1 gctcgccaga accgtattga tatttactga gcgctcagat caactttcca tggcaacaga
       61 tcgcgtaccc ggtttgccgg ccagtcctgg atatgctccc gccgacaata ttaatggcca
      121 ggaaatgttt aaaacaatga ttctgtttcc taaccatcaa gtgtatatgc tggctcaatt
      181 actcaatatc tatgggttga gttctaccca aagtggctct gtagtcgctt cttcttacag
      241 tatgttcagg tgttgcaggc acagttgtgg agcgtagatg cgttgttggt ttaccatgct
      301 ctggtacata aaaaacgcca gtgccatggt aacctgatat agaaacgtta attccgcgac
      361 gttcaaactc ggcatagacg tgttctgcta aagacctctt ttgaccaaat aatgccctgg
      421 ccaaccagcc gttattcata tttgcggatt tttctatttc atcaggagag aagtctttgt
      481 tttttattat gttggctgag ttacaggatg ttaatctgat atcatctatt tcatgaagat
      541 tatgttgaac aatgcggtcg acaatatctg acggtgataa aaattcatct ccgcacttaa
      601 gaagaggtag accagcagaa ccatgcccag acagataaat tttgttgtat tttcctggtg
      661 ttaaatcatc tggcaggatg gttttcatct ctgtcgctgt aatacttata gctgcagcag
      721 caacaacatc gctattactt gattgtagat ggcttttatt tgctccagga tatgtaaact
      781 ccattttcct tttatcaaaa tcttgttttg tagcatcatt caggaataat aaatcatacg
      841 gttttttccc cgttgtgact cttgaaacat tttggccaag aatattaaga gtatagtcat
      901 taacggattt cattcctaaa aaaacaagta atccttcttt ctcttgcgct ttttcgattg
      961 ttctccctgc taaagttatt gggatggatt gagattgttt tgtcggaact gatacgctgg
     1021 cgctaatagg tagtagggtt cttatgctaa acatacaacc tctttcttaa ttttcgaacc
     1081 tgtttaggat tctgtgtaaa ttcaaaataa acctgtctga acattcctaa caaacatcca
     1141 ccggacatga caacaaaaac cggagccgga ctccggtttt tgtgaagctg tcggctattt
     1201 cattc
//
LOCUS       DAZGJW010000471         1187 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZGJW010000471 DAZGJW010000000
VERSION     DAZGJW010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.475"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..245)
                     /locus_tag="ACHKIK_005612"
     CDS             complement(<1..245)
                     /locus_tag="ACHKIK_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9086513.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLKAQGGERYFFCNELNE
                     KGEPVTWQGRKYQAYPIDGSGFEMNGKGSSARPSLTVS"
     gene            complement(245..586)
                     /locus_tag="ACHKIK_005613"
     CDS             complement(245..586)
                     /locus_tag="ACHKIK_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9086514.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEPTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            complement(579..>1187)
                     /locus_tag="ACHKIK_005614"
     CDS             complement(579..>1187)
                     /locus_tag="ACHKIK_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9086515.1"
                     /translation="SDYASQAAQLATEGISGMVNNITEMLNGNKVEWRNWASSVLQEI
                     SKVLMNAAIVNGIKTAANGMSGAGGFIGSIGDWLGGAVANAKGGVYTSANLSAYSNSI
                     VDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIA
                     IQNDGANGQIGAEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
BASE COUNT          239 a          419 c          262 g          267 t
ORIGIN      
        1 gacaccgtca gcgacgggcg ggcactgctg cccttcccgt tcatctcaaa gccactgccg
       61 tcaatcgggt atgcctggta cttccgcccc tgccaggtca ccggctcccc tttttcattc
      121 agctcattac agaaaaaata ccgctcacca ccctgggcct tcaggtcaat ttcccagagc
      181 accacccgcg gtgactgctc tgacttaacc gactcgttca gactttcttc gcgaatatcc
      241 tgcatcagtt caccacctgc tcaatcgtac agctgaaatc actgtacctg gcgttatctg
      301 tgacgctcca ctcccggcac accaccctca ccgtccggtt atgtttcggc ggtcgccaca
      361 aaaaggcacg gtaaccacca tgccaggata aaaattcatc cagccagcgc cgggttggtt
      421 catccgtcac ccggaacacc gcctgaaacg tcttcagtct ggcattaagt cccgtcggtc
      481 ggcgctgttc ataaccgtca ccaaaccgaa ccctcaccac cgacggtttc tcactcacct
      541 gcatcccttc acgcgggacc agatgcagcg tttttatctc agccactcag cattcctccg
      601 tcacgtcgca tggacagcat caccgcctgc acccgctggt caatcagctg cacaagactg
      661 cctgccgcct ccgcccctat ctgtccgtta gccccgtcat tctgaatggc gatgtggtag
      721 accggggaat acaccagacc ggcactgccg ttcatactgc ccaccgcgcg tacgcccagc
      781 gagccatccg ccgcccgggt caggggcata atggcttcag gtccggcttc ccccatcagc
      841 ccggcccctt ttgcaaacgc aaagtacgtg ggcgtgtcca caatgctgtt gctgtacgca
      901 ctcaggtttg ccgaggtata cacgccgcct tttgcattgg ccaccgcacc gcccagccag
      961 tcaccaatgc tgccaataaa tcctcccgca ccggacatac cgtttgccgc cgtcttaatt
     1021 ccgttgacaa tcgcggcatt cataagaact tttgagattt cctgcagtac ggatgaggcc
     1081 cagttgcgcc attccacttt gtttccgttc agcatctccg tgatgttatt caccatccct
     1141 gagataccct ccgtcgcaag ctgtgctgcc tgtgaagcgt aatcgga
//
LOCUS       DAZGJW010000472         1181 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZGJW010000472 DAZGJW010000000
VERSION     DAZGJW010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.476"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1181
                     /locus_tag="ACHKIK_005615"
     CDS             <1..>1181
                     /locus_tag="ACHKIK_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO9086516.1"
                     /translation="PRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTAL
                     VGVQVDLEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWC
                     LWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQR
                     KAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSA
                     LKDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGL
                     WLIKTELLETQTVDFSVGAEGLRHVPGDVIEIFDDDYAGISTGGRVLAVNSQTRTLTL
                     DREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGLKVKVNRVPDGVAEYSVWGLKLPT
                     LR"
BASE COUNT          263 a          310 c          376 g          232 t
ORIGIN      
        1 ccgcgcccgt ttaatatccg gatgcgcagg atgacgccgg acagcaccac agaccagctg
       61 cagaacaaaa cgctctggtc gtcatacacc gaaatcatcg atgtgaaaca gtgctacccg
      121 aacacggcac tggtcggcgt acaggtggac ttggagcagt tcggcagcca gcaggtgagt
      181 cgtaattatc atcttcgcgg gcgcattctg caggtgccgt cgaactataa cccgcagacg
      241 cgacaataca gcggtatctg ggacggaacg tttaaaccgg catacagcaa caacatggcc
      301 tggtgtctgt gggatatgct gacccatcca cgctacggca tggggaaacg tcttggtgcg
      361 gcggatgtgg acaaatgggc gctgtatgtc atcggccagt actgcgacca gtcagtaccg
      421 gacggctttg gcggcacgga gccgcgcatc acctgtaatg catacctgac cacacagcgc
      481 aaggcgtggg atgtgctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac
      541 gggcagacgc tgacgttcgt gcaggaccga ccatcagata aggtgtggac ctataaccgc
      601 agtaatgtgg tgatgccgga tgatggcgcg ccgttccgct acagcttcag cgccctgaag
      661 gaccgccata atgccgttga ggtgaactgg attgacccga acaacggctg ggagacggcg
      721 acagagcttg ttgaagatac gcaggccatt gcccgttacg gtcgtaatgt cacgaagatg
      781 gatgcctttg gctgtaccag ccgggggcag gcacaccgcg ccgggctgtg gctgattaaa
      841 acggaactgc tggagacgca gaccgtggat ttcagcgtgg gtgctgaagg gcttcgccat
      901 gtaccgggcg atgttattga aatcttcgat gatgactatg ccggtatcag caccggtggt
      961 cgcgtgctgg cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg
     1021 ccatcctccg gtaccacgct gataagcctg gttgacggaa gtggtaatcc ggtcagcgtg
     1081 gaggttcagt ccgtcaccga cggacttaag gtgaaagtga accgggttcc tgacggcgtt
     1141 gcagaataca gtgtgtgggg gctgaagctg ccgacgttgc g
//
LOCUS       DAZGJW010000473         1178 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZGJW010000473 DAZGJW010000000
VERSION     DAZGJW010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.477"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(543..>1178)
                     /gene="nleL"
                     /locus_tag="ACHKIK_005616"
     CDS             complement(543..>1178)
                     /gene="nleL"
                     /locus_tag="ACHKIK_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO9086517.1"
                     /translation="KDGEVQNTFGYSLSNLFSESFPIFSIPYHKAFSQNFVSGILDIL
                     ISDNELKERFIEALNSNKSDYKMIADDQQRKLACVWNPFLDGWELNAQHVDMIMGSHV
                     LKDMPLRKQAEILFCLGGVFCKYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQVF
                     GSVYYYNDILDRLQGRNNVFTCTAVLTDMLTEHAKESFPEIFSLYYPVAWR"
BASE COUNT          440 a          243 c          147 g          348 t
ORIGIN      
        1 ttgagagtct tttcaaaaca atcattaatt acgccatatt actatagtaa caaaaacata
       61 ttaatctgaa tatcaattca taaaatatga ttctccacga tttgcccaga aataacgaac
      121 ataattttcg ttgtaattaa aatcatattt catctatagt gaccattcac agcaaaacaa
      181 aatcaattat cacattcaaa aaaaatactg acaacaaaga tactcttcag acaaagttgt
      241 catatgtaaa ttttccctta aaacgttgct caaaaaaaat ataaaaatca tatgccctaa
      301 tcaacatcct caagcactac gatatatgca gtatcctatt catatattac caccatatca
      361 gacaaatcga atcacaacga gttctatcgt actctttcat cttcgacact gtaaatttta
      421 agaacattcg gctcatgcca ctaaaccaag acataataac aacggagcaa taaatgttcc
      481 cactgaacat gtgcaaacct aaaaagacag tgttacaata atattcatcc atggtctcta
      541 aatcaacgcc acgcaacagg ataatacaat gaaaatattt caggaaaaga ttcttttgca
      601 tgctccgtta gcatatcagt cagcacagcg gtacaagtaa aaacattatt tcttccttgt
      661 agcctgtcta aaatatcatt gtaataataa actgagccaa atacctgagg atctgtttta
      721 taagcttgtt caatcaatcc atttgcatat ctccgtagaa tctcaggaga atcatactct
      781 gtaccaaaca tatcactcga tgagtattta cagaaaaccc cccctaaaca aaataatatt
      841 tcagcctgtt ttcttagtgg catatctttc aatacatggc tccccataat catatctaca
      901 tgctgagcgt tcagttccca accatcaaga aagggattcc agacacaggc aagtttcctt
      961 tgctgatcat cagcaatcat tttataatct gatttattgg aattaagtgc ctcaataaat
     1021 ctctctttga gttcattatc agaaatgagt atatccagaa taccagaaac gaaattctgg
     1081 gaaaaagctt tatgataagg aatactgaaa attggaaatg attcagaaaa aagattgctc
     1141 aatgaatacc caaatgtatt ttgaacttct ccatcctt
//
LOCUS       DAZGJW010000474         1164 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZGJW010000474 DAZGJW010000000
VERSION     DAZGJW010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.478"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>1164)
                     /locus_tag="ACHKIK_005617"
     CDS             complement(<1..>1164)
                     /locus_tag="ACHKIK_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO9086518.1"
                     /translation="RNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGL
                     RHTPGDIIEICDNDYAGTMTGGRVLSIDAASRTLTLDREVTLPETGTSAVNLINGSGK
                     PVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAIT
                     AVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRV
                     VKGVRFSLRLTSGKGTDARLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPA
                     TTTFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQVETAA
                     RYLGSALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGRASDDAEGYLDF"
BASE COUNT          224 a          363 c          337 g          240 t
ORIGIN      
        1 aaaatccagg taaccttccg catcatcgct cgcccgaccg acagcctcca cgaatgccga
       61 tttgccaaca gtattcacac tgcggatata aaaataataa tcatggcccg gtttgatatt
      121 gatactggca gctatccagt acagcgccga gccaagatag cgggctgcgg tttcaacctg
      181 cctgatatcc gcaatccgct tttccgagaa ccagaactca aactgtaccg tcgggtcata
      241 aacggcaaga tgcggcgtgg cggttatctg aaaatagccc ggcgtcagct caatccgcga
      301 cggtgctgcc ggtgcggcaa tccggaaggt ggtggtggca ggttcaccct gctggccata
      361 gctgtttatc gcccgcaccg tcagggtgta ttccccgagc ggcaggccgc tgaaacggtg
      421 ctccgtgtct gcggtgatgg cggtggtcac cagtctggca tccgttccct taccactggt
      481 caggcgcaga ctgaagcgca cgcccttcac cacccgcggc gtgtcccatt tagcctgcgc
      541 cagatactgg ccgtcagctg cactcacctc caccgtcagg tgctgtactg ccggtgggat
      601 gacgctgttc agggaacctg actgcggctc aaagcgggca ccgttatcca cgatggcttc
      661 tttttccggt acgtgctgca ccgccgtgat ggcaaaggtg ccgtccgtgt tttcccggac
      721 ggagacacag cggaacaggc gacggcgcag tgacggcagg gagagtcccc acaccccgta
      781 tgtctccaca ccatcaggca gggtactgac ctgtatccgg tccggcgcgg ggtgtgcggt
      841 gatgtccaca ctcaccggct taccgctgcc gttaatcagg ttcaccgccg atgtacctgt
      901 ctccggcagg gtcacctcac ggtccagtgt cagggtgcgg ctggcggcat cgatggacag
      961 gacacgtccg ccggtcatgg taccggcgta gtcgttatca cagatttcaa taatgtcacc
     1021 gggtgtgtgc cgcagcccct gagacccgag cgtgaaatcc accgtctgcg tttccagcag
     1081 ttcggtcttt atcacccaca gtccggcacg gtgggcctga ccgcggctgg tacagccgaa
     1141 cgcgtccatc ttcagcaggt tgcg
//
LOCUS       DAZGJW010000475         1155 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZGJW010000475 DAZGJW010000000
VERSION     DAZGJW010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.479"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..865
                     /locus_tag="ACHKIK_005618"
     CDS             <1..865
                     /locus_tag="ACHKIK_005618"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO9086519.1"
                     /translation="IKPVWALANDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHS
                     NYGAALEKQGVEITLIYSGSHKVDGNPYSHLPDDVRETLQYRMDATRQMFAQKVSAYT
                     GLSVQAVLDTEAAVYSGQEAIDAGLADELVNSTDAITVMRDALDARKSRLSGGRMTKE
                     TQSTTVSATASQADVTDVVPATEGENASAAQPDVNAQITAAVAAENSRIMGILNCEEA
                     HGREEQARVLAETPGMTVETARRILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPAS
                     DAVNDLLNTPV"
     gene            875..>1155
                     /locus_tag="ACHKIK_005619"
     CDS             875..>1155
                     /locus_tag="ACHKIK_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO9086520.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAAS"
BASE COUNT          256 a          339 c          356 g          204 t
ORIGIN      
        1 cataaaaccg gtatgggcgc ttgccaacga catgaactgc agtgcaggtc agttgcttgc
       61 cagtgccgcc tcccggcgtc tggtcacgca gaccgcccgg acaggctcca tcggcgtcat
      121 gatggctcac agtaattacg gtgctgcgct ggagaaacag ggtgtggaaa tcacgctgat
      181 ttacagcggc agccataagg tggatggcaa cccctacagc catcttccgg atgacgtccg
      241 ggagacactg cagtaccgga tggacgcaac ccgccagatg tttgcgcaga aggtgtcggc
      301 atataccggc ctgtccgtgc aggctgtgct ggataccgag gctgcagtgt acagcggtca
      361 ggaggccatt gatgccggac tggctgatga acttgttaac agcaccgatg cgatcaccgt
      421 catgcgtgat gcactggatg cacgtaaatc ccgtctctca ggagggcgaa tgaccaaaga
      481 gactcaatca acaactgttt cagccactgc ttcgcaggct gacgttactg acgtggtgcc
      541 agcgacggag ggcgaaaacg ccagcgcggc gcagccggac gtgaacgcgc agatcaccgc
      601 agcggttgcg gcagaaaaca gccgcattat ggggatcctc aactgtgagg aggctcacgg
      661 acgcgaagaa caggcacgcg tgctggcaga aacccccggt atgaccgtgg aaacggcccg
      721 ccgcattctg gccgcagcac cacagagtgc acaggcgcgc agtgacactg cgctggatcg
      781 tctgatgcag ggtgccccgg caccgctggc tgcaggtaac ccggcatctg atgccgttaa
      841 cgatttgctg aacacaccag tgtaagggat gtttatgacg agcaaagaaa cctttaccca
      901 ttaccagccg ctgggcaaca gtgacccggc tcataccgca accgcgcccg gcggattgag
      961 tgcgaaagcg cctgcaatga ccccgctgat gctggacacc gccacccgta agctggttgc
     1021 gtgggatggc accaccgacg gtgctgccgt tggcattctg gcggttgctg ctgaccagac
     1081 cagcaccacg ctgacgttct acaagtccgg cacgttccgt tatgaggatg tgctctggcc
     1141 ggaggctgcc agcga
//
LOCUS       DAZGJW010000476         1138 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZGJW010000476 DAZGJW010000000
VERSION     DAZGJW010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.480"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..103
                     /locus_tag="ACHKIK_005620"
     CDS             <1..103
                     /locus_tag="ACHKIK_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086521.1"
                     /translation="AIKMRKYEPYLLNDNSILSRIALLELGIIGEQQ"
     gene            100..288
                     /locus_tag="ACHKIK_005621"
     CDS             100..288
                     /locus_tag="ACHKIK_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO9086522.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYDSKSSCIQVRDEQKIPGE
                     CLPLKKVSLNLNNEIPAG"
     gene            384..>1138
                     /locus_tag="ACHKIK_005622"
     CDS             384..>1138
                     /locus_tag="ACHKIK_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO9086523.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFIPVEPESDPM
                     NVNFDKLSLEVQNAVLVKFGTCENITVDMAIDAQELLQEDVATFDGHIVEALMKTPEI
                     NAMYPERKLFAIGWVKHKCNPGAKWPEIQAELRNWKKRQDAERKETGKYTSVVDLASA
                     RVNQQNTENSAGKINPVTAAICREYKQTWKTLD"
BASE COUNT          347 a          266 c          262 g          263 t
ORIGIN      
        1 tgccattaag atgagaaaat atgaacctta tcttctcaat gataattcca tactttccag
       61 aattgccctt cttgaacttg gtattatcgg agaacagcag tgacttcagc atttgcactg
      121 gtgatgaccg tttttcttat aacgggtgag ccacaaaatg tgattaccgg aatttatgac
      181 agtaagtcat cctgcattca ggtaagggac gaacaaaaaa tccccggtga atgcctcccg
      241 ttaaaaaaag tatcgctgaa cctgaataac gaaataccgg ctggataacc cgccagccat
      301 attaacgcca taccaacgga ttaaaaatgc cagcaatggc agggattcgt tcaccctgaa
      361 atctgtaatg aggttaaaac aaaatgagta aagtctttat ttgcgccgcc attccggacg
      421 aacaggcaat aaaggaagaa ggtgccgtcg ctgtagccac tgccattgaa gccggtgatg
      481 aacgtcgcgc ccgcgcaaaa tttcactggc aattcctgga acattatccg gctgctcagg
      541 actgcgctta taaatttctt gtctgcgagg ataaacccgg tataccccgc cctgccctcg
      601 attcctggga tgctgaatat atgcaggaaa accgctggga tgaggaatcc gcttccttta
      661 ttccggtcga accagaatcc gatccgatga acgtcaattt tgacaagctg tcccttgaag
      721 tacagaacgc ggtcctggtt aagttcggta catgtgaaaa catcaccgtt gatatggcga
      781 ttgacgcgca ggaattactg caggaagacg ttgctacctt tgacgggcat atcgttgaag
      841 cactgatgaa aacgcctgaa attaacgcta tgtatccgga acgcaaactg ttcgctatcg
      901 gatgggttaa acacaaatgt aatccgggtg ccaaatggcc cgaaattcag gctgaattac
      961 gtaactggaa aaaacggcag gacgcagagc gcaaagagac tggaaaatac acgtctgttg
     1021 ttgatctcgc cagcgccaga gtcaatcaac agaacactga aaactcagca ggaaaaatca
     1081 acccagtcac tgccgccatt tgtcgcgaat acaagcagac atggaaaacg ctggatga
//
LOCUS       DAZGJW010000477         1133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZGJW010000477 DAZGJW010000000
VERSION     DAZGJW010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.481"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            142..396
                     /gene="yjbL"
                     /locus_tag="ACHKIK_005623"
     CDS             142..396
                     /gene="yjbL"
                     /locus_tag="ACHKIK_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFO9086524.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRYRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            420..1127
                     /locus_tag="ACHKIK_005624"
     CDS             420..1127
                     /locus_tag="ACHKIK_005624"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO9086525.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNLGDNK
                     DAMPIEQCTFDVENKNDLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
BASE COUNT          394 a          163 c          225 g          351 t
ORIGIN      
        1 tgcttcaagt taactcgcta tttatttaac tgggtttaaa cgcatacctg tagttcagaa
       61 attatgagcg atttttgata gtgaccagat ggcaaaagaa ttataaggag gacacggtac
      121 gacaaaaaag gtagatgaat catgttgaag ataatacccg gtgcaactgg atactttaat
      181 aaaacactca attcaaatca attcgataat gaagatgcaa tcaaagataa attagataat
      241 agaggttcaa tcaaaggtaa attaaataat atatatggta aatctattga ttatgcagcg
      301 ctgcgttatc gggatataat tattgcaaaa atagatttgt ttattcaacg aatcacgcat
      361 aatttatggc atgcacgtaa aaaaatgtgt ttttaattga gcaaataaat gatctcaaaa
      421 tgtgggtcaa taagtatatt gacgattgca ctgatgagga tttaaacgat cgtgacttta
      481 ttgcatcagt tgttgaccgg gctatttttc atttcgcgat taatagtata tgtaatcttg
      541 gggataataa agatgcaatg cccattgaac aatgtacttt tgatgtagaa aataagaatg
      601 accttccctc cacggttcag ctattttatg aggaatctaa ggataatgaa cctttagcga
      661 atatacattt tcaagcaata ggttctggtt ttttaacgtt tgttaatgcc tgccaggaac
      721 atgatgacaa cagcttaaaa ttatttgctt cgctgttaat ttcactttca tattctagtg
      781 cctacgcaga tttatcagaa acagtgtata ttaatgaaaa taatgagagc tacctgaaag
      841 ctcagtttga aaaattatct caacgtgata tgaagaagta cctgggagag atgaagcgtc
      901 tggctgatgg gggagaaatg aattttgatg gctatctgga taagatgtca catctggtga
      961 atgaaggaac gctcgatcct gatattttaa gcaaaatgcg agatgctgca ccacaattaa
     1021 ttagcttcgc gaagtcgttt gacccaacct caaaggaaga gattaaaata cttacagaca
     1081 cttctaaatt aatttatgat ttgttcgggg ttaaatcgga gaaataatat gtg
//
LOCUS       DAZGJW010000478         1132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZGJW010000478 DAZGJW010000000
VERSION     DAZGJW010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.482"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..202
                     /locus_tag="ACHKIK_005625"
                     /pseudo
     CDS             1..202
                     /locus_tag="ACHKIK_005625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807328.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            331..576
                     /locus_tag="ACHKIK_005626"
     CDS             331..576
                     /locus_tag="ACHKIK_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086526.1"
                     /translation="MDIRHSLNEWWTLWQASRAAIELDIDWPESNDDFWKDGEEGAYA
                     MGYEDGRDKTVIAVMKAIRAAGIKEKNFDEANNIPPN"
     gene            548..958
                     /locus_tag="ACHKIK_005627"
     CDS             548..958
                     /locus_tag="ACHKIK_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO9086527.1"
                     /translation="MKQTIFLRTKQQQQAAINAILATPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCDKWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            955..1131
                     /locus_tag="ACHKIK_005628"
     CDS             955..1131
                     /locus_tag="ACHKIK_005628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO9086528.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
BASE COUNT          341 a          229 c          294 g          268 t
ORIGIN      
        1 tgagtttatg ctcgtcgcac tcaaatgcgt tggcgtttga tggattcttc tgacgttatt
       61 tattgttctg catagctaca ttcgtcttgt gaatgacggt aaagacccat ggtatacgtt
      121 gtttggcgct gcatttgtct gggtgattat cggtgttgcg cctgtcgctg tagcaaaaat
      181 ggcgtggcgt tttgtgagtt gaactgaggg taagtatcga tggacgaatc aagaaagcag
      241 tttgaggaat acgttgccaa aaaattgaga ttaccattcg agatgataac cgaggcaaga
      301 aatggtgata ggtacttcgc attttcaagc atggatattc gtcactcctt aaatgagtgg
      361 tggactttat ggcaggcatc gcgagcagct attgaactgg atatcgactg gccagaatcg
      421 aatgacgact tttggaaaga tggtgaagaa ggtgcttatg cgatgggtta tgaggatggg
      481 cgtgacaaaa cggtaattgc agtaatgaaa gctatcagag ccgctggaat taaagagaag
      541 aatttcgatg aagcaaacaa tattcctccg aactaagcaa caacagcaag ccgcaatcaa
      601 cgccatcctc gcaacaccac tcgataaaga caagccagtc accatccgca ttactgacta
      661 caagcgcaac cttgaccaga acgcaaaatt tcacgcgatg ctggcggata tcgcacgtca
      721 ggttcaatgg tgcgataagt ggttaaaacc agaacaatgg aaggttttgt tgattagcgg
      781 tcatgcagtg gcaacaaagc aggaagctga tgttttgccc ggccttgaag gcgaatacgt
      841 caacattcgc gaaagtagcg cgcagatgag tgtgaagcgt atggcaagtc tgattgagta
      901 cacgacagca tgggctattg gtcagggcgt cagatttacc gacaggaggt acgaatgaga
      961 cgacagcgac gaagtatcac cgacataatc tgtgaaaact gcaaatacct tccaacgaaa
     1021 cgctccagaa ataaacgcaa gccaatccca aaagaatctg acgtaaaaac cttcaactac
     1081 acggctcacc tgtgggatat ccggtggcta agacatcgtg cgaggaaatg ac
//
LOCUS       DAZGJW010000479         1131 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZGJW010000479 DAZGJW010000000
VERSION     DAZGJW010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.483"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(22..327)
                     /locus_tag="ACHKIK_005629"
     CDS             complement(22..327)
                     /locus_tag="ACHKIK_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO9086529.1"
                     /translation="MATGSKNAKSQSLTARIPHDVIEGMESVKLDGESNAGFIVTAMR
                     GEIARRQAEGSGENLLISSLNALAQVEKLGIKAAEEIGQLVTVAREELQRRKTKEPE"
     gene            524..724
                     /locus_tag="ACHKIK_005630"
     CDS             524..724
                     /locus_tag="ACHKIK_005630"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO9086530.1"
                     /translation="MRNHTTHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RLQSPAGCVMVFAARIRQGVGL"
     gene            721..951
                     /locus_tag="ACHKIK_005631"
     CDS             721..951
                     /locus_tag="ACHKIK_005631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086531.1"
                     /translation="MSQEITLQQAVERAHQIEVICALAEDYPGMMTDSESGAIIGLLK
                     RLSGEVCVFLIDEQERRTLISNEKKCGGVHVQ"
     gene            941..>1131
                     /locus_tag="ACHKIK_005632"
     CDS             941..>1131
                     /locus_tag="ACHKIK_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086532.1"
                     /translation="MCNKTTPDAAAAALTTLMHALIDIECTAELAQKEEREEYTLFAL
                     ECIRYTATRSLNDAKNILVA"
BASE COUNT          308 a          288 c          276 g          259 t
ORIGIN      
        1 acaccacggc gctgatggtg attactctgg ttctttagtc ttgcggcgct ggagttcttc
       61 acgtgcgaca gtgacgagct gcccgatctc ctcggcggct ttgattccga gtttttccac
      121 ctgcgccagt gcattgagcg aagaaatcag gaggttttct ccgcttcctt ctgcctggcg
      181 gcgggcgatt tcaccgcgca tggcggttac tatgaatccg gcgttgcttt caccgtccag
      241 ttttacggat tccatgcctt caataacatc atgcgggatc cgagctgtca gtgattgtga
      301 ttttgcgttt tttgaacctg tagccatctg taatcctctc aatgaaagtg taagacaata
      361 tacacataaa aagtcttaca taaaagcatt gacatgtaag ccacctataa ataaagttac
      421 ttacaccttg ttaatgcaag gtgcagaaac gacgaaaccc cgcactgtag gagcagtaac
      481 ggggcttcta accaccaacg ataacgagag tatcgaggta gctatgagaa atcataccac
      541 acacccgcaa gggcgggact cgcacaacct gaataaatac atttggcgtt ttatcgccct
      601 gagcactgca caaccgcgcg tgattaccat tgaggccacc agcgaacagg aagcacgcct
      661 gcaatccccg gctggctgcg tgatggtatt cgctgcccgt attcgtcagg gggtaggctt
      721 atgagccagg aaatcacact acaacaggca gtagaacgcg cccaccaaat cgaagttatt
      781 tgcgcactgg cagaggatta cccaggcatg atgaccgaca gcgaatcagg ggcaatcatc
      841 ggcttactta aacgccttag cggtgaggtc tgcgtattcc tgatcgatga acaggaaaga
      901 agaacgctta tttctaacga aaaaaaatgc ggaggggtac atgtgcaata aaaccacacc
      961 ggacgcagcc gccgccgcac tcactacgct gatgcacgcg cttattgata ttgaatgcac
     1021 agcagagctt gcacaaaagg aagaaaggga agaatacaca ctattcgccc tggaatgtat
     1081 ccgatacacc gcaacgcggt cgctgaatga cgctaaaaat attcttgttg c
//
LOCUS       DAZGJW010000480         1127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZGJW010000480 DAZGJW010000000
VERSION     DAZGJW010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.484"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..779
                     /locus_tag="ACHKIK_005633"
     CDS             <1..779
                     /locus_tag="ACHKIK_005633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO9086533.1"
                     /translation="TRHEPVIGIMGKSGAGKSSLCNALFQGEVTPVSDVHAGTREVRR
                     FRLSGHGHSMVITDLPGVGESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDEY
                     FWRHILQCGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKTEAVFRLFRPV
                     HPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRAQAREQFTGAV
                     DRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFF"
BASE COUNT          247 a          308 c          303 g          269 t
ORIGIN      
        1 tcacccgtca cgaacccgtg attggcatta tgggtaaaag cggggccggt aaatcctcac
       61 tctgtaatgc actgtttcag ggggaggtca ccccggtcag tgatgttcac gccggcaccc
      121 gggaagtacg gcgcttccgt ctgagtggcc acggtcacag catggttatc actgacctgc
      181 ccggggtggg cgagagccgg gacagggatg cagagtatga ggccctgtac cgtgacattc
      241 tgcctgaact tgacctggta ctgtggctga ttaaagccga tgaccgtgcc ctgtctgtgg
      301 atgagtattt ctggcgacac atcctgcagt gcggacatca gcaggtgctg tttgtggtga
      361 cgcaggccga caaaacggag ccctgccatg aatgggatat ggccggtatt cagccttctc
      421 ctgcacaggc acagaatatt cgcgaaaaaa cggaggcggt attccgtctg ttccggcccg
      481 tacatccggt tgtggccgta tcggcccgca ccggctggga actggatacg ctggtcagtg
      541 cgctcatgac agcgcttccc gaccatgccg ccagtcccct gatgacccga ctgcaggacg
      601 agctgcgcac ggaatctgtc cgcgctcagg cccgtgaaca gtttaccggt gcggtggacc
      661 ggatatttga cacggcggag agcgtctgtg ttgcctctgt tgtccgtacg gccctgcgcg
      721 ctgttcgtga cacggtggtc tctgttgccc gtgcggtatg gaactggatt ttcttctgaa
      781 cctgtcgtga ctgatgccct ccctgactct gagtctgctc acaaaagcac tgttttcgtt
      841 actgtctctc ttgtccgtgc aatagctcaa taatagaata aaacgatcaa tatctatttt
      901 atcgatcgtt tatatcgatc gataagctaa taataacctt tgtcagtaac atgcacagat
      961 acgtacagaa agacattcag ggaacaacag aaccacaatt cagaaactcc cacagccgga
     1021 cctccggcac tgtaaccctt tacctgccgg tatccacgtt tgtgggtacc ggctttttta
     1081 ttcaccctca atctaaggaa aagctgatga aacgacatct gaatacc
//
LOCUS       DAZGJW010000481         1118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZGJW010000481 DAZGJW010000000
VERSION     DAZGJW010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.485"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..428
                     /locus_tag="ACHKIK_005634"
     CDS             <1..428
                     /locus_tag="ACHKIK_005634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO9086534.1"
                     /translation="AVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSAL
                     AAGGFSATTMLFSLGASMILGGVAQMLAPKAKVPEYKSTDNGRQNTYFSSLDNMIAQG
                     NPMPVPYGEMLVGSRRISQDISTRDEGGDGKVVVIGRQA"
     gene            664..>1118
                     /locus_tag="ACHKIK_005635"
     CDS             664..>1118
                     /locus_tag="ACHKIK_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9086535.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEK"
BASE COUNT          269 a          260 c          365 g          224 t
ORIGIN      
        1 gggcggtcat ccatattgtg ccgcgactgg ccggagccgg aaagggcgga ctgcagattg
       61 tgctgggggc ggcagccatc gtggggtcgt tcttcacggc cggggcatca atggcgttat
      121 ggggttcagc cctggcagcc ggtggttttt ctgccaccac gatgctgttt tcactggggg
      181 ccagcatgat actgggtggt gtggctcaga tgctggcccc gaaggctaaa gtaccggagt
      241 acaaaagcac ggataacggc agacagaaca cgtacttttc ctcgctggat aacatgattg
      301 cacaggggaa cccgatgccg gtgccttacg gtgaaatgct ggttggctca cggcgaatct
      361 cccaggacat cagtacccgt gatgaaggcg gtgacgggaa ggtggtggtt atcgggcggc
      421 aggcataaaa gcgaaaaaat cccgcagtga ccgaaggctg cgggaacaga aaatgaagat
      481 taaccacagg gagttttgtt tttattggcc cgaaaaaact gtaacgcccg ggaatgatat
      541 ctgccacggg ggcgtacaga aaatgtgaag atattcagaa tttttattca gtcatgatac
      601 aggcatcctc cgggatgcct gttgtttttg tgcgtaacag ttatcacagt aaagggtgag
      661 acaatgggca aaggtggcgg caaggcgcac acgccggttg aggcaaagga caatcttaag
      721 tccacgcaga tgatgagcgt gattgatgcg attggtgaag ggccgattga aggtccggtg
      781 aaggggctgc agagtatcct ggtgaacaaa accccgctga cggacacgga cggtaatcct
      841 gtgatacatg gtgtgacagc ggtctggcgc gccggggagc aggagcagac accacctgaa
      901 ggctttgagt cctccggggc ggaaaccgca ctgggcgtgg aagtgacgaa agcaaagccg
      961 gtgacgcgca ccattacgtc cgcgaacatt gaccgcctgc gggtcacctt cggggtgcag
     1021 tcactgttgg agaccacctc aaagggcgac cgtaatccct cttctgtccg actgctgatt
     1081 cagttgcagc gtaacggtaa ctgggtgacg gaaaagga
//
LOCUS       DAZGJW010000482         1118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZGJW010000482 DAZGJW010000000
VERSION     DAZGJW010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.486"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..919)
                     /locus_tag="ACHKIK_005636"
                     /pseudo
     CDS             complement(1..919)
                     /locus_tag="ACHKIK_005636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            896..1118
                     /locus_tag="ACHKIK_005637"
                     /pseudo
     CDS             896..1118
                     /locus_tag="ACHKIK_005637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          268 a          324 c          287 g          239 t
ORIGIN      
        1 tacccgccgt acacggtgtc gtactgggta gcgtatgtat ttttccagta atacgtcgtg
       61 tcaccacaaa cccacggcac atctgcagca ctgccaccat ggcactgcgc gttaaacacg
      121 gagaggtcag cacgaaactg tgtcagcatg gctgtaaaca gcgcaggttg ctgtacgtgg
      181 gtggcggcgc tcatgtcaaa ctcaccctgc atccagcaga cggccagcag aacgtttttg
      241 ggatttttct gcaatgccgc ttttgtgcgg gaaatcagat cctgatataa cggcttgccc
      301 accccccagc gtgccgaatc ctgactggcc cccgtggact cgctgaatgt cccctccgcg
      361 ccctgggtaa atgccgaacc accacgacag catggtacca gcaggatccc cgcgttattc
      421 gggatatacg ggagcagttt tttggcaata tgtaaaccct ggccgacaca gccgtactgc
      481 cctttgctca ggtcagccct cggatgattc agcgtactca tatcctgcac atcatgcaga
      541 cagtggtcag ccggaataat atcgttatat ctgcaggcag ccccgcccgg cgtcactgta
      601 ctgcggcgcg ccagctgttt aatgcgcgga tccggagcat cgtatgaatc cggcagcgga
      661 agcccttcac cgtaagccct tcaccgtaag ccatggcatt ggattgcccg gccagtacga
      721 tgacgtagta ccaatccggc tcagatgaag ggccgacctg tggctctcct tcaatagcca
      781 ccgcctgcat cagtgtgtac ggcgtaatgg caaccggtcc gccgtatggc tgccagccct
      841 ctttcagttt gtgtgtcagc ttttccgcaa ggtctgacgg cgacgccgcc ctgacaacat
      901 cgtaatgttt aatcgacatc gaatttctcc cgtgtagagg aacagagtta aaaagccgga
      961 agcggaatca aatcacagga tgaccatctg ccagtggcag gtcataaaaa aaaggctgcg
     1021 caatgcgcag ccagaactca caaggaaaat gataaaagga ataacactag tgatgtacgc
     1081 atggcgcctc ccgctaagtt ctgcaatgat caaacaga
//
LOCUS       DAZGJW010000483         1108 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZGJW010000483 DAZGJW010000000
VERSION     DAZGJW010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.488"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1107
                     /gene="ygeH"
                     /locus_tag="ACHKIK_005638"
     CDS             <1..1107
                     /gene="ygeH"
                     /locus_tag="ACHKIK_005638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFO9086536.1"
                     /translation="IETIYRKGYRFSGQVFKTKINEDNTSDYSIAIFPFTTSLNTLDP
                     LILNQELVQIISNKKIDGLYTYPMAATNFCNDHISQNSFLSRFKPDYFVTGRINQNNA
                     VNTLYIELIDAKNLFLIASNHLPVDELHNTSQFIIDNILQTVHKPERSVRLAKQDQGY
                     KNHYLSDEMLAGKKELYDFTPESIYRAMTIFDILQNKSDIQTLKTECYCLLAECHMSL
                     ALHGKSELELAAQKALELLDYVSDITTVDGKILAIMGLITGLSGQAKVSHILFEQAKI
                     HPTDIASLYYYRALVHFHNEKIEEARICIDKSLQLEPRRRKAVVIKECVDMYVPNPLK
                     NNIKLYYKETESESHRVIIDNILKLKQLTRICMR"
BASE COUNT          409 a          203 c          183 g          313 t
ORIGIN      
        1 atagaaacaa tctaccggaa aggttatcgt ttcagcgggc aggttttcaa aactaaaatt
       61 aatgaagata atacttcaga ctattccata gctatattcc ctttcactac ttcgttgaat
      121 acactggatc cattaatact taatcaggaa ttagtgcaaa tcatttcaaa taaaaaaatc
      181 gatggtctct atacctatcc gatggctgcg acaaattttt gtaatgatca catatctcaa
      241 aattcattct tgagcagatt caagccagat tatttcgtta caggaagaat aaaccagaat
      301 aatgcagtga acactttata cattgagttg atcgacgcta aaaacctttt cctcatcgcc
      361 agtaatcatc tccctgttga tgaactacat aatacatcac aatttattat agataatatc
      421 cttcaaacgg tacataaacc agaacgatct gtaagattag ctaagcagga ccaaggatat
      481 aagaatcatt atttatcaga tgaaatgtta gccggaaaga aagaacttta cgacttcacc
      541 cctgaaagca tttacagggc catgactata tttgatatat tacaaaataa aagtgatata
      601 cagacgctaa aaacagaatg ttattgcctt ctagcggaat gccatatgtc tttggcactt
      661 catggaaaaa gtgaacttga acttgctgct caaaaagcat tagagctttt agattatgta
      721 tcagacataa ccactgtcga tggaaaaatt ttagctatta tgggactgat aactggtctg
      781 tctggacaag caaaagtatc tcatatctta tttgaacagg ctaagataca cccaactgat
      841 atagcctctc tctactacta tagggcacta gtccactttc ataatgaaaa aattgaagag
      901 gcaaggattt gtatagacaa atcactacaa ctcgaaccca gaagacgaaa agcagttgtg
      961 ataaaagaat gtgtagatat gtatgtgcct aacccgctca aaaacaacat taaactctac
     1021 tataaagaaa ctgagagtga aagccatcga gttataattg acaacatttt gaaattaaag
     1081 cagctgacga gaatttgtat gcgataaa
//
LOCUS       DAZGJW010000484         1078 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZGJW010000484 DAZGJW010000000
VERSION     DAZGJW010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.489"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..740)
                     /locus_tag="ACHKIK_005639"
     CDS             complement(<1..740)
                     /locus_tag="ACHKIK_005639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO9086537.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AVAREQRRALAELNSQLTEIRASAAGTAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLEVLRDEYA"
     gene            complement(788..997)
                     /locus_tag="ACHKIK_005640"
     CDS             complement(788..997)
                     /locus_tag="ACHKIK_005640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO9086538.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
BASE COUNT          233 a          362 c          257 g          226 t
ORIGIN      
        1 gcgtattcat cacgcaatac ctccagctgg ctgattaacg caggaacgac tttatccgcc
       61 gtcagttgac catcgtccgc catcgccttc agatctttac gggccacgcc catacccgca
      121 gccagtgcac gtacgatccg gtcaccactt tcattgaccg aattaaattc ctcaccgcgc
      181 aacacaccct gtgccagcgc ctggctgaac tgggtgatca ccgaacccgc ctcagccgta
      241 ctggcaccgg agattttcag ccccgtggaa atggcctccg tcaccttcag cacatcatca
      301 gcactgtaac catattcacg cattgaggct gccgagcggg caaacagggc cgcattatcc
      361 gaaaaagcgg tacctgtccg ctggctgata tccatcagca ctttctgtga tgacgaaaat
      421 tcatcggatg actgcgacgc ctgtttcagt cgggcattca cggaactcca ttcatcggcc
      481 agagaaatca ggtgtccggt ggcaaaggca cctgcaaatg cccccgccgt tccggcagct
      541 gaagcgcgga tttccgtcaa ctggctgttc agctcagcca gggcgcgtcg ctgctcccgg
      601 gcgactgcgg cagcctgacg cccgccattc tgcagggtcc ggtaatattc actgcccatg
      661 cgggaagccc gctggatctc cgactggaat gactgcgaat ttgccgaaat tttgataatc
      721 agttcacgta acgtcgccat tcacctttct ccgggcgtaa aaaaaccgcc tcagcggttc
      781 tcatcattca tgactgtgct gcgaagctca gcgcgtcttc cagcgccgca aacggatcca
      841 cctccggctt atcctcatcc tcgccccagc agagcatggc gtccttcagt gcaacattca
      901 tcccctgtgc cccaaaaacc gctttcacga tctgtgcatt acggatatcc ccgcgctcat
      961 cacccagcgg ggataccctg tcgaactcca tccacatcat cgcctcgctc acactcaggc
     1021 tgtgccgcag ttcggataag gtgcgcccca gacggagcgc aagtcgcatc agaaagcg
//
LOCUS       DAZGJW010000485         1076 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZGJW010000485 DAZGJW010000000
VERSION     DAZGJW010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.490"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..329)
                     /locus_tag="ACHKIK_005641"
     CDS             complement(<1..329)
                     /locus_tag="ACHKIK_005641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9086539.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDIIPGKTYTKAECKALLNKDLATVARQINPYIEVDIPETTRGAL
                     YSFVYNVG"
     gene            complement(329..544)
                     /locus_tag="ACHKIK_005642"
     CDS             complement(329..544)
                     /locus_tag="ACHKIK_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO9086540.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            687..>1076
                     /locus_tag="ACHKIK_005643"
     CDS             687..>1076
                     /locus_tag="ACHKIK_005643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086541.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNNISDVRMAPPAKFCNDQTELAT"
BASE COUNT          290 a          278 c          223 g          285 t
ORIGIN      
        1 cccacgttgt aaacgaatga gtaaagagcg ccgcgcgttg tttccggtat atcgacttcg
       61 atgtacgggt taatttgtct ggcgacagtg gcaaggtctt tattcaagag tgctttgcat
      121 tctgctttgg tatacgtttt accgggaatg atgtcttttc cggtgtgtcc gtgacataca
      181 gtccatacgc caacgatatc ttcgtatggt atgtagctga caccttccag gccatcgtca
      241 ccactcggac cagtgatgag cacagacgct atggcaacag ccccaccacc aatagcagca
      301 gcaacagcct tgcgtaatga tggcgacatt attcacctct cgcagcctta cgcttatctt
      361 ctttaatctt gaaataaagg tttgtcagat acgtcagcaa gccaaacacc aggctaccca
      421 atacgcctat tgccacccac tgggatgggg agactttgtc cagcaactgc agtagccagt
      481 atcccgtccc caccgctgac gtggtgtatg acacacctgt tgttattttt tccatctggt
      541 acataccccg tctcccgtta tccggaagct gacaacaata aaaagccacc agttaattcc
      601 tgatggccct gatgcataaa cgtcataata cctgactgtt atgattgaca ataatgataa
      661 tgtttatata gaaaggttcc cgatgtgtgt tacatatcat ttctccacgg ggaatatccc
      721 cacgccagcg cagactcttt tacccgttct cttctgcgct ggctcttttt tattatgctg
      781 ctgcatttac ctctggcacc atgctttcta tctcaacaca atacgtggta cttcttgtaa
      841 ccaatatcat aacgattaat cgacatagaa tttctcccgt gtacaggaac agagttaaaa
      901 agccggaacc ggaatcaaat cacaggatga ccatctgcca gtggcaggtc ataaaaaaaa
      961 ggccgcgcca tgcgcagcca gaactcacaa ggaaaatgat agaaggaaat aacattagtg
     1021 atgtacgcat ggcgcctccc gctaagttct gcaatgatca aacagaactc gctacg
//
LOCUS       DAZGJW010000486         1076 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZGJW010000486 DAZGJW010000000
VERSION     DAZGJW010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.491"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..174)
                     /locus_tag="ACHKIK_005644"
     CDS             complement(<1..174)
                     /locus_tag="ACHKIK_005644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO9086542.1"
                     /translation="MSIKHYDVIRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGEPQVGPS"
     gene            151..294
                     /locus_tag="ACHKIK_005645"
                     /pseudo
     CDS             151..294
                     /locus_tag="ACHKIK_005645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(489..656)
                     /locus_tag="ACHKIK_005646"
     CDS             complement(489..656)
                     /locus_tag="ACHKIK_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO9086543.1"
                     /translation="MTVKLRLTVAALLLFLVVMVDFTSRIMSVLADGVLVCGIMVLLW
                     PVIKRNSLHNA"
     gene            complement(653..1076)
                     /locus_tag="ACHKIK_005647"
                     /pseudo
     CDS             complement(653..1076)
                     /locus_tag="ACHKIK_005647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310776.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
BASE COUNT          275 a          312 c          234 g          255 t
ORIGIN      
        1 tgaagggccg acctgtggct ctccttcaat agccaccgcc tgcatcagtg tgtacggcgt
       61 aatggcaacc ggtccgccgt atggctgcca gccctctttc agtttgtgtg tcagcttttc
      121 cgcaaggtct gacggcgacg ccgccctgat aacatcgtaa tgtttaatcg acatcgaatt
      181 tctcccgtgt agaggaacag agttaaaaag ccggaagcgg aatcaaatca caggatgacc
      241 atctgccagt ggctggtcgt aaaaaaaagg ccgcgccatg cgcagccgaa aataaaggga
      301 taacgatgat agtttgagaa aaacagaaat aacacttttg tggcaaagca tggtgccggg
      361 tgcctcccgg tgaattcagt atcagcacct gaatccgcga ttaccccata ttccttcttg
      421 ctgattgccc caccgcacag ggggattcac catgcagaag tgtttttaat aaacagcaaa
      481 caaaaaaatc aagcattatg caggctgttt ctttttatca ccggccacag caataccata
      541 atgccgcaga ccagcacccc atccgccagc accgacatga ttctgctggt gaaatccacc
      601 atcaccacca gaaacagcag gagtgcagcc acagtcaggc gcagttttac cgtcacaggt
      661 aattctccag acgaagaccc agaacaccgg caatctcttc cagcaccttg cgctcttccg
      721 gctcaatttc gccgtctgcc tccgcaatgg ccaccgccac atccagcaca tcttccgctt
      781 cacgcgtatc gtgtttcaca tcctcgatct cacgtaacgc cgcacgacga ccagttttaa
      841 agttcgtatc cagctgaccg ataatggttg cgctaatcgc attaatttct gacgtaaacg
      901 cgtacagcgc aggctgatta cgcagtacct gttcgatctt cgctttctag gaagcctcac
      961 attcaccatc tgcataggcc accaggtagg cggcgttaat caccacctgt gccagatcgc
     1021 gtttttcaaa ctttttaatt tccgttgccg ctctgcgggc tttttttacc aaaaat
//
LOCUS       DAZGJW010000487         1074 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZGJW010000487 DAZGJW010000000
VERSION     DAZGJW010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.492"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1074
                     /locus_tag="ACHKIK_005648"
     CDS             <1..>1074
                     /locus_tag="ACHKIK_005648"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFO9086544.1"
                     /translation="EAIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQP
                     EWITYSPSVMYSISKAIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENG
                     RGYVINWQDFELKIKSAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIH
                     SDFVFNHSFTSALTIKDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYR
                     DFVNSPSVLNVIATIVAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGC
                     YFSWVDCSALGYPFDKFYRRLINEGRVGIMAGHVYGTEGEGYLRLNVACSREKLYMGL
                     ARLASVIKNINQEKENEQYGSYP"
BASE COUNT          344 a          178 c          229 g          323 t
ORIGIN      
        1 cggaagctat catcagaaca ttaaaaaaac gtctggaaca cccgatttta gggtacagtc
       61 gttggcagca tgatgactat ctgaattcga tagtcaattg gtatcatcaa caatataaaa
      121 cagagattca accagaatgg atcacctata gcccaagcgt gatgtattca atatctaagg
      181 caatagaaat attgacattg aatggagata atattctcat tttcacgcct gtttataacg
      241 cattttttga tgtgattaag cataatggtc gaaacatgct aacaacctct ttgatgaaag
      301 aaaatgggag aggctacgtt atcaactggc aagactttga gcttaaaatt aaaagcgcga
      361 agatggtgtt attatgtaat cctcataacc cgacaggaac ggtctggtcg gaagacgatc
      421 ttcacagaat cgcagcatgc tgtgcaaggc ataatatttg gttatgttca gatgaaatac
      481 atagtgattt tgtttttaat catagtttca cgtcagcgct aacaataaaa gatgacaaag
      541 tggtggtgtt taattcgatt tcgaaaacat ttaatgttcc tgcgctaacc ggatcatata
      601 tgatttcaac tgacgataat tttaatcata agttcagaac gatatcgagg tatcgcgatt
      661 tcgtaaactc cccatcagta ctgaatgtaa ttgcgaccat tgttgcctat aatgaatgtg
      721 aagaatggtt aaagtcgtta aaggtacata ttgcctctaa cattcaattt accttgcagt
      781 atttaaatga aaatattccg gaattgatcg tatggccagc tgagggctgc tatttttcgt
      841 gggttgattg ctcggccctt ggttatccct ttgataaatt ttaccgtcga ttaattaacg
      901 aagggagagt aggtattatg gcggggcatg tttatggtac tgaaggagag ggctatctgc
      961 gtcttaatgt ggcctgcagt cgagaaaaat tatatatggg gcttgcgcgc cttgcgagtg
     1021 taattaaaaa tattaatcag gaaaaagaaa atgaacaata cggatcttat ccat
//
LOCUS       DAZGJW010000488         1067 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZGJW010000488 DAZGJW010000000
VERSION     DAZGJW010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.493"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            351..788
                     /locus_tag="ACHKIK_005649"
     CDS             351..788
                     /locus_tag="ACHKIK_005649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUKH-3 domain-containing protein"
                     /protein_id="HFO9086545.1"
                     /translation="MCELINEASKYGKVNERKMDIDLCCKKIEEEGYSVFVAFYDFIK
                     KYGGLYGFHSAYKDTTINEAFFIDPERASNRIFIERVNDYIERAGYNMIPVGECSNGH
                     ITLLYGENNVIYGGLDDYLWKYGDNLSSSIDILINGKEAVEIP"
     gene            808..1067
                     /locus_tag="ACHKIK_005650"
                     /pseudo
     CDS             808..1067
                     /locus_tag="ACHKIK_005650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
BASE COUNT          323 a          195 c          262 g          287 t
ORIGIN      
        1 tgtaaaagat ggttatttag aggaaataat tttgtctggt tcataaattc atcttctttg
       61 ttgagaagat taactgccta ggtgaaatta tcctctatga ccgggacgct gcaggccacc
      121 tggcagaagt catcacgcag gaagacaagt cgctgtatcc ctttgaaaaa tccggcaggc
      181 taatgggcat cttcagccca ggcggcacat cacagcgaat ttgaaggcaa atgcgaaaat
      241 tagtgatctt gaagttcata cagtattggt aaaaacaggc gaagcttttc caatgtgtaa
      301 gaattttcaa atttcaatat ttggcgctgt tgttacaagc gggtgattat atgtgtgaat
      361 taattaatga agcatcaaaa tatgggaaag tcaatgaaag aaagatggac attgatttgt
      421 gctgtaaaaa aatagaggaa gaaggatatt ctgttttcgt tgcgttctat gattttataa
      481 aaaaatatgg cggtttatat ggctttcatt cagcttacaa agacaccact ataaatgagg
      541 ctttttttat agatccagaa agggcctcta atcgcatttt tatagaaaga gtcaatgatt
      601 atattgagag agctggttac aatatgattc ctgttggcga gtgcagtaat ggtcatatca
      661 cattattata tggggaaaat aatgtgatat atggcggctt ggatgattat ctttggaagt
      721 atggggataa tttatcttca tcaattgata tattgattaa tggcaaggag gcagttgaaa
      781 ttccgtaagg ccaacttgat atgacacctg gcacacaacc ccgacgaccg gatgtatatg
      841 ctgggcggga tgagtgacac cgccagcaac cgcatcacgt ttgagcgcga cagccagtac
      901 cggatcacgt gtgtcagtca caccgacggg atccggctta aactgacgta ccatgccagc
      961 ggctacctga aagccattca ccgcacggat aacggcatac agacgctggc gacctacgaa
     1021 caggatgcgc gggggcggct gaccgaagcg gatgcgcggc tggacta
//
LOCUS       DAZGJW010000489         1065 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZGJW010000489 DAZGJW010000000
VERSION     DAZGJW010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.494"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1065
                     /locus_tag="ACHKIK_005651"
     CDS             <1..>1065
                     /locus_tag="ACHKIK_005651"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFO9086546.1"
                     /translation="IFPDRFCNCRPEISPEGVEPWGSAPTSFNFMGGDLWGVIDKLDY
                     LEDLGINGIYFCPIFTSASNHKYDTIDHFSVDPHLGGNIAFHTLIEEAHKRGIKIMLD
                     AVFNHLGADSLIWLDVVRNGANSRYADWFWIHKFPVYPDTPKSEWDFKNFNYETFGNV
                     IEMPKLNTENEECREYLLSIVRYWTQNFDIDGWRLDVANEVDHHFWRDFRKVIKDIKP
                     ECYILGEIWHEGTPWLRGDQFDSLMNYPLTYGIIDYFALQDTTKQEFMTSVTRSYLCY
                     PKNITEVMFNLLDSHDTARILSVCLNDKRKVKLAYLFMLTQAGSPCIYYGSEIGIDGF
                     KSMTLENNRKCMIWDENKQDL"
BASE COUNT          316 a          172 c          226 g          351 t
ORIGIN      
        1 atttttccag accgtttctg taactgcaga cctgaaatat ccccagaagg ggtagaaccg
       61 tggggcagtg caccgacttc attcaacttt atgggtgggg atctgtgggg ggttattgat
      121 aaactcgatt atctggaaga tctgggtatt aatgggattt atttttgtcc tatttttacc
      181 tcagcttcca accataaata tgacaccatc gatcatttta gtgttgatcc acatttaggt
      241 gggaatattg ctttccatac tttgattgaa gaagcgcata agcgtggaat aaaaatcatg
      301 ttggatgcag tattcaatca tttaggcgct gactctctaa tatggcttga cgttgtacgc
      361 aatggtgcca attcacgtta cgccgattgg ttctggattc ataaatttcc tgtttatccc
      421 gatactccaa aaagtgaatg ggattttaaa aactttaact atgaaacctt tggcaatgta
      481 attgaaatgc ccaagctaaa tactgagaat gaagagtgcc gtgaatactt acttagtata
      541 gttcgctatt ggacgcaaaa ttttgatatt gatggttggc gtctggatgt tgcgaatgaa
      601 gtggatcatc atttctggcg agatttcaga aaggtgatta aagatatcaa acctgaatgt
      661 tatattttgg gtgagatatg gcatgagggc accccctggt tgcgtggtga tcagtttgat
      721 tcattaatga attatccctt aacatatggg attattgatt attttgcgtt acaggataca
      781 acaaaacaag aatttatgac atccgttaca cgttcttatc tttgttatcc taaaaatata
      841 actgaagtta tgtttaatct tctggatagt catgacaccg cgcgtatttt aagcgtttgt
      901 ttgaatgata aacgaaaagt taaactcgcc tatttattta tgttaactca agcgggctct
      961 ccttgtatct actacggtag cgaaatagga attgatggtt ttaaatcaat gacgcttgag
     1021 aataatagaa aatgtatgat atgggatgaa aacaaacaag atctt
//
LOCUS       DAZGJW010000490         1057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZGJW010000490 DAZGJW010000000
VERSION     DAZGJW010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.495"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1057
                     /locus_tag="ACHKIK_005652"
                     /pseudo
     CDS             1..1057
                     /locus_tag="ACHKIK_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
BASE COUNT          375 a          199 c          196 g          287 t
ORIGIN      
        1 taacatctct aaggatgaat gggaaaacct ccatgcatta aaagaaaagc gtttaaatgg
       61 aatgcctaaa acaacaccca aaagtgacca ggttattatg ttgcaaaatc aatacgttcg
      121 tgaaatgcga aaatatggcg tacgaggttt acgttatgat gcggcaaaac actcaaaaca
      181 tgaacaaata gaaagatcaa taaccccacc gcttaaaaat tataatgagc ggttacacaa
      241 tactaactta tttaacccaa aatatcataa aaaagccgtt atgaattaca tggaatatct
      301 ggtaacttgt cagttggatg aacaacaaat gtcatcactg ctttatgaaa gagatgattt
      361 aagcgccatt gatttttcat tgctcatgaa aacgataaaa gccttttcat ttggtggaga
      421 tcttcaaacc cttgcatcaa aaccgggttc cacaatctca agtatcccat cagaaagacg
      481 gatattgatt aacattaacc acgattttcc taacaatggt aatcttttca atgactttct
      541 atttaaccat caacaagatg aacaattagc aatggcatat atagccgctc tgccgttcag
      601 caggccttta gtttactggg atggccaagt attaaaatca acgactgaaa ttaaaaatta
      661 tgatgggtcc acgcgtgtcg gcggtgaggc gtagcttaat aaaggttgct ctacctatca
      721 gcagctctac aatgaattcc acgcattata tatagataaa gcaggaatat ggagcgcatt
      781 tgagggtgta tctgcaacta aaaacgttct ggcctttagt cgtggggatt ctgtgaacat
      841 taatcactct cctcatgatg gactagttat aataaataaa ggaaacgaag aagttgaagg
      901 tacctggcct aacaaattgc aacctggaat atacaaaaac atggggagta atagcgttaa
      961 cattattatt aataataccc gaaaaattat cccccctggt aaagtattta cgcttagagg
     1021 cggaactcta aatatcaata ttcctgggcg tagcgct
//
LOCUS       DAZGJW010000491         1051 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZGJW010000491 DAZGJW010000000
VERSION     DAZGJW010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.496"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            179..472
                     /gene="iss"
                     /locus_tag="ACHKIK_005653"
     CDS             179..472
                     /gene="iss"
                     /locus_tag="ACHKIK_005653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFO9086547.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(563..745)
                     /gene="rzoD"
                     /locus_tag="ACHKIK_005654"
     CDS             complement(563..745)
                     /gene="rzoD"
                     /locus_tag="ACHKIK_005654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO9086548.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(962..>1051)
                     /locus_tag="ACHKIK_005655"
     CDS             complement(962..>1051)
                     /locus_tag="ACHKIK_005655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9086549.1"
                     /translation="RWTYAGGKQWKGLMTRREIEREICLWGQQ"
BASE COUNT          270 a          252 c          236 g          293 t
ORIGIN      
        1 ctaaaagggt aatggacaga tagagcggtt tatttcattc cacaggattc tgagtgcccc
       61 cctcctccaa taggctgagc atccacctat atagttttaa ttttcatcaa tccatttaac
      121 tatcgtttaa ttgttgtcac ataggattct gccgttttta acaatgcagg ataataagat
      181 gaaaaaaatg ttgttttctg ccgctctggc aatgcttatt acaggatgtg ctcaacagac
      241 gtttactgtt ggaaacaaac cgacagcagt aacaccaaag gaaaccatca cccatcactt
      301 cttcgtttcg ggaattggac aggagaaaac tgttgatgca gccaaaattt gtggcggcgc
      361 agaaaatgtt gttaaaacag aaacccagca aacattcgta aatggattgc tcggttttat
      421 tactttaggc atttatactc cgctggaagc gcgtgtgtat tgctcacaat aattgcatga
      481 gttgcccatc gatatgggca gctctatctg cactgctcat taatatacgt ctgggttcct
      541 tccagttgtt tttgcatagt gatcagcctc tctctgaggg tgaaataatc ccgttcagcg
      601 gtgtctgcca gtcgggggga ggctgcatta tccacgccgg aggcggtggt ggcttcacgc
      661 actgactgac agactgcttt gatgtgcaac cgacgacgac cagcggcaac atcatcacgc
      721 agagcatcat tttcagcttt cgcatcagct aactccttcg tgtattttgc atcgagcgca
      781 gcaacatcac gctgacgcat ctgcatgtca gtaattgccg cgttcgccag cttcagttct
      841 ctggcatttt tgtcgcgctg ggctttgtag gtaatggcgt tatcacggta atgattaaca
      901 gcccatgaca ggcagacgat gatgcagata accagagcgg agataatcgc ggttactctg
      961 ttcattgctg accccacaaa cagatttcac gctcaatctc acgacgagtc atgagacctt
     1021 tccattgctt accgccagca tatgtccagc g
//
LOCUS       DAZGJW010000492         1031 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZGJW010000492 DAZGJW010000000
VERSION     DAZGJW010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.497"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..129
                     /gene="kil"
                     /locus_tag="ACHKIK_005656"
     CDS             <1..129
                     /gene="kil"
                     /locus_tag="ACHKIK_005656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO9086550.1"
                     /translation="HQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINLPWYRRK"
     gene            205..375
                     /locus_tag="ACHKIK_005657"
     CDS             205..375
                     /locus_tag="ACHKIK_005657"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086551.1"
                     /translation="MSLATTVKESKLQRRMYTQQALMYRQKGDREGVRVFLNAAKTEV
                     LNQRYFLGPCPF"
     gene            386..991
                     /locus_tag="ACHKIK_005658"
     CDS             386..991
                     /locus_tag="ACHKIK_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFO9086552.1"
                     /translation="MSKDFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAALQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
BASE COUNT          349 a          212 c          249 g          221 t
ORIGIN      
        1 caccagatgc ttcgtgcggc acaaaacaaa gcagtaatag ccagatttat tggtgattca
       61 aaaatgtggc ttgaagcaaa taaagcgatg aaatcagcta tcaaccttcc gtggtatcgc
      121 aggaaatgag tttcacagat aactggtcag acgaagaatt cattcgtcag atgaaagaat
      181 taatcggtaa cgaaggagat attcatgtca cttgcaacca cagtgaagga gagcaagtta
      241 cagagacgca tgtacacgca gcaggcgtta atgtatcgcc agaagggaga tcgtgaaggt
      301 gttcgcgtat ttttaaatgc ggcaaagact gaagtattaa atcagcgtta tttccttggg
      361 ccatgcccat tctgagaaca aacatatgag caaagacttt tacgcaagac tggcagctat
      421 tcaggagaac ttgaacgcgc caaagaatca gtacaactca ttcggtaaat ataaatacag
      481 aagctgcgaa gacattcttg aaggcgttaa gccgttactg aatggcctgt ttttatcaat
      541 cagcgatgaa gttgtgttga ttggtgatcg gtattatgtg aaagccacgg caactattac
      601 cgatggcgaa aacagtcata cggcaaccgc tcttgcacga gaggaagaaa gcaagaaagg
      661 aatggattct gcacaagtta cgggagctac aagctcttat gcacgcaagt attgcctcaa
      721 tggtttgttc ggcattgatg atgcgaaaga tgcagatacc gacgagcata aacatcagca
      781 gaacgcagca gcaaagcagt caaaaccatc acctacacct gaacaggttc taaaagcatt
      841 cactgacgca gcattgcaga aaaacaccgt ggaagagctt aaacaggcgt tcgccaaagc
      901 gtggaagatg ctcgaaggca caccggagca gcacaaagcg caggacgttt acaacatcag
      961 acgagacgaa ttagaaggag ctgctgctta atggcacatt cgattactgt aagactaaac
     1021 aagcccgcaa g
//
LOCUS       DAZGJW010000493         1022 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZGJW010000493 DAZGJW010000000
VERSION     DAZGJW010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.498"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(497..682)
                     /locus_tag="ACHKIK_005659"
     CDS             complement(497..682)
                     /locus_tag="ACHKIK_005659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO9086553.1"
                     /translation="MRELKMKLCVLMLPLVVSACASTPTVQAPCVKPPSPPAWIMQPV
                     PDWQKPLNGIISSSENG"
     gene            complement(910..1020)
                     /locus_tag="ACHKIK_005660"
     CDS             complement(910..1020)
                     /locus_tag="ACHKIK_005660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086554.1"
                     /translation="MAKILCVIVGISFLVILVAIFFSTAWRVLTLSGLVG"
BASE COUNT          310 a          261 c          188 g          263 t
ORIGIN      
        1 cctgaattta ccttccctta atgaaataac aataaaaaac aaaccacgca aaaacaataa
       61 aacaacacac aaaaaaaact aaataataaa caaaaataat caccttgttt tattattttt
      121 tgagggagca attactgaac aaaaaacgct gactatatac tcaaaaccaa acaactattc
      181 tgccaatcag gtatcatggc aacacacgga attaccgtgt ttttgccttc tctgcccata
      241 caatacgggc atatacttca ttctctattg taatatttct atccatgtgc cccactccat
      301 ttacctgtaa ataatattca aaatatttat cacagaaatc gtttttggcc atgaactgag
      361 cacactataa agtccggaac tgactctttg ttaaattacc ttaacgttac cagtaacacc
      421 ttcgtaacaa aacatcacgg tatacactgg gtacggatat attcctgtgc tccttccagt
      481 tgcttctgca ttgccatcag ccgttctctg aggatgaaat aatcccgttc agcggcttct
      541 gccagtcggg gaccggttgc attatccacg ccggaggtga tgggggcttt acgcaaggag
      601 cctggacagt tggcgttgat gcgcaggcgc ttacgaccag cggcaacatc agcacgcaga
      661 gtttcatttt cagctctcgc atcggctaat tccctcgagt atctggcatc aagtgcagca
      721 acatcacgct ggcgctgctg catatcagta atggttgcat ttgccagctc cagctcactg
      781 acttttttat cgcgctgctc tttgtaggtt atggcgttat cgcggtaatg attcagcccc
      841 agactaagtg caccacaggc taccagcagg acaataatca ccacacacag aacacggttc
      901 atctctcttt caccccacca gtcccgataa cgtcaggact cgccaagcgg tggaaaagaa
      961 aatggcaacc agaatgacta aaaatgaaat gccgacgatt acacagagga tcttcgccag
     1021 cg
//
LOCUS       DAZGJW010000494         1015 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZGJW010000494 DAZGJW010000000
VERSION     DAZGJW010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.499"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(147..755)
                     /locus_tag="ACHKIK_005661"
     CDS             complement(147..755)
                     /locus_tag="ACHKIK_005661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFO9086555.1"
                     /translation="MEKEYGTAVAQELVTGLLSGQDYIKRNPDTEAMAKAQSIMNTWG
                     YHKSNASIGDAPLLFGGSVLGLTVKGMATNAAIGISVNSAAQLAGKDPFSYVDAIIAG
                     VTAAATTGKGIIVSTPINMSGAAIGSSIKGEDPTNSVIGTGLGSVFGGGIGKVISGSM
                     GSTVKEGTSDIIGNIGGSITGEAIGNVTKGTLDEVDKSIKNK"
BASE COUNT          282 a          250 c          158 g          325 t
ORIGIN      
        1 taatatccgc cctccaatca aaaaataaaa cttgagtttt agaaaaaact atcagtctac
       61 caagaatcgt acctaacatt gccataaaaa agatgcaagc tatgcagcgc gttattagta
      121 tgataagaat gtaaaagttg acactcttat ttatttttaa tggatttatc aacttcatct
      181 agtgttcctt ttgttacatt cccgatagct tcaccagtaa ttgatccccc aatattacca
      241 ataatatcag atgttccctc tttgacagta gagcccattg accccgagat gactttccct
      301 ataccgccac caaatactga acctaatcct gtcccaataa ctgaatttgt cggatcctcg
      361 cctttaatac tactaccaat tgccgcgccg ctcatgttga taggggttga aactataatt
      421 ccttttcctg ttgttgctgc tgccgttacc cctgcaatta tcgcatccac ataactgaaa
      481 ggatcttttc cagcaagctg agcagctgag tttacgctga taccaattgc tgcattagtc
      541 gccatacctt tcactgttaa ccccagtacg cttcctccaa acaacaaagg agcatcacca
      601 atacttgcat tagatttatg atacccccag gtattcataa tgctttgagc ctttgccatt
      661 gcttcagtat cagggttacg ttttatatag tcctgtcctg acagtaaccc agtaacaagc
      721 tcctgtgcca ccgctgtacc gtactccttt tccatttctg aagcatatac acgcaagtaa
      781 gctgccagtt cagcacgttc tgcgctgttt aattgtgaag gatctttatt aaatttggat
      841 accagagcat cactacgttt atctgcattt tccagcttaa tcagctcatt agcagtggct
      901 aatgtcttat cgccgttcct gattttctca atggctttat ccagtttgtc agaagatgtc
      961 gctcccagaa agttattctc aacaactatt ttttattttt aaagtaaaac tctac
//
LOCUS       DAZGJW010000495         1005 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZGJW010000495 DAZGJW010000000
VERSION     DAZGJW010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.500"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..164)
                     /locus_tag="ACHKIK_005662"
     CDS             complement(<1..164)
                     /locus_tag="ACHKIK_005662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HFO9086556.1"
                     /translation="MRLASRFGYTNQIRRERPLTHEELMHYVPSIFGEDRHTSRSERY
                     AYIPTITVLE"
     gene            complement(254..328)
                     /locus_tag="ACHKIK_005663"
                     /pseudo
     CDS             complement(254..328)
                     /locus_tag="ACHKIK_005663"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            296..435
                     /locus_tag="ACHKIK_005664"
                     /pseudo
     CDS             296..435
                     /locus_tag="ACHKIK_005664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(575..>1005)
                     /locus_tag="ACHKIK_005665"
     CDS             complement(575..>1005)
                     /locus_tag="ACHKIK_005665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086557.1"
                     /translation="NGVFIMKNIITILMIAVISFFTANVSANNAVPLPTNLTDVTDGT
                     EFWCQGTDTIDGGCEYLGTSKDMQYGLVHAMGGTACWDGLYGVINFYTGKAQTIKFNN
                     NQSCEGNITAGFVTLKNGKPGVKLYDNTIHKIVGLDEIKI"
BASE COUNT          278 a          207 c          231 g          289 t
ORIGIN      
        1 ttttccagga cggtgatggt gggaatgtac gcatagcgtt cactgcggga ggtatgccgg
       61 tcttctccaa aaatactggg aacatagtgc atcagctctt catgtgtcag tggacgttca
      121 cggcgtatct ggtttgtata accaaaacga ctggctaatc gcataattgc tccttatgaa
      181 tgattaagcg gtataaatga aaaaaccgcg tctcccggag aagacgcggc ctgatgggtg
      241 aaatgaatga ggtttattgt ccgggaagcc cttcactggt gagccacgca ttaagttgtt
      301 tgcctgcata gggttcagaa ttccggcagc gccagcgtcg ctgaccttcc gcgttgcgga
      361 taatccgccg gaaatgcatt cctggataaa cacattgcgg taagctgtta tgacagcaac
      421 tgtctgtttg cgtgagaaag gatcttcgga aaataaaagt aatgtacagc ccgtcatgta
      481 ttttcaggta aaaataaagc ccctgccatt atggcagagg atttatttac atttgatatt
      541 aataacgcgc agataagata ctataattca tacattaaat tttgatttcg tcaagcccga
      601 caatcttatg aatcgtattg tcgtaaagtt taacgccagg tttaccgttt tttagagtaa
      661 cgaaaccggc ggtgatgttg ccttcacaac tttgattgtt gttgaatttg atagtctggg
      721 ctttaccggt atagaaatta attacgccgt ataaaccatc ccagcacgct gtacccccca
      781 tggcgtgtac cagaccgtat tgcatgtctt tggatgtgcc caggtattca caacctccgt
      841 cgatagtatc agtaccctga caccagaatt ctgtaccgtc agtaacgtca gtcaggttgg
      901 tcggtaaagg aacggcattg tttgctgata cattagcagt aaagaaactg ataactgcaa
      961 tcattaaaat agtgatgata tttttcatga taaatactcc attgt
//
LOCUS       DAZGJW010000496         1002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZGJW010000496 DAZGJW010000000
VERSION     DAZGJW010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.501"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            171..803
                     /locus_tag="ACHKIK_005666"
     CDS             171..803
                     /locus_tag="ACHKIK_005666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9086558.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQY"
BASE COUNT          341 a          168 c          182 g          311 t
ORIGIN      
        1 cataatcata tttaataacg agaaatgcat tttaaaatgt cttaatcaga attatagcaa
       61 atacagatta atcgcaattt ttcccagcaa aattacaaaa tcatggtgta ttgacatcat
      121 caatcaccaa tgaaattatg caattatata cggataggga ggttcttaac atggggcttt
      181 gtagtcgtta taaaagtctt acatgcaata gttgctcaat gcattgccaa ataatgccag
      241 aagagtcacc gcgtttgcag tactgcgcaa actcgtgttt ttgtatgtgg cccgaagaaa
      301 gttcatattt taatcgtggt gtggtagaag gtatcttaac aaaaaaccac aatgcgagat
      361 taagcggata tatttttgtc gatttttcag taagtttttt acgtctattc cttgaaaaag
      421 actggattga ctatcttgca agtactgaca tggggattgt tttagtcagc gaccgcaata
      481 tgcaatcgtt agctaattac tggcgaaaac acaactcagc aatatctgct gtcatatata
      541 atgatgatgg tcttgatgtt gccaatgaaa agatcagaca actatttatt ggtcgttatt
      601 tatcatttac cagaggtaac acattaactc agatggaatt caccataatg gggtatatgg
      661 tttctggtta taatccatat cagattgctg aagttctgga tatggatatc cgtagcatct
      721 acgcgtacaa gcaacgaatc gaaaagagaa tgggtggtaa aataaacgaa ttatttattc
      781 gttcacattc ggtccaatat tgatactcaa cgaccagcca gaatcatact ctgtttatac
      841 gggcatttat tgaaatgtcc gtattttata aataggccgt cccctcaaga gtaaacacca
      901 tagtatttaa aaccatcaca cgaaaaattc agaagcatta cgaaatatga atttacaaac
      961 aatagtggca taaatgttaa ccatgttaat ttacgtaaag tt
//
LOCUS       DAZGJW010000497          996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZGJW010000497 DAZGJW010000000
VERSION     DAZGJW010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.502"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..166
                     /locus_tag="ACHKIK_005667"
     CDS             <1..166
                     /locus_tag="ACHKIK_005667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO9086559.1"
                     /translation="TDGALITGLTFLDPKDATQVQGLFQHLQVRFGDGPWQDVKGLDE
                     VGSDTGRTGE"
     gene            168..437
                     /locus_tag="ACHKIK_005668"
     CDS             168..437
                     /locus_tag="ACHKIK_005668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO9086560.1"
                     /translation="MNILKKLMQRLCGCGKHDDREHGELLTVQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGRWENIGGMK"
     gene            550..>996
                     /locus_tag="ACHKIK_005669"
     CDS             550..>996
                     /locus_tag="ACHKIK_005669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO9086561.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEAIQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAF"
BASE COUNT          263 a          180 c          282 g          271 t
ORIGIN      
        1 tacagatggt gcgctcatca ccggactgac ctttcttgac cccaaagatg ccacacaggt
       61 tcagggactg tttcagcatt tgcaggtcag atttggcgac gggccgtggc aggatgtcaa
      121 ggggctggat gaagtgggca gtgatacagg cagaacagga gaatgacatg aatatactaa
      181 aaaaacttat gcagcgtctg tgcggttgcg gaaagcatga tgaccgtgaa cacggggagt
      241 tacttacagt acaactgcga ctggggccgg cagacatcct ggagtcagat gagaatggca
      301 ttattccgga gcaggacagg gtaatcacac aggtggtgat actggatgcg gataaaaagc
      361 agatacagtg cgtggtaaga ccgctgcaaa tcctacgtgc tgacgggagg tgggaaaata
      421 ttggcgggat gaaatagccg acgggttcac aaaaaccgga gtcctgctct ggtttttgtt
      481 gtcacgtatg ggggatgttt gttacagcta tttaagtctg gagttcaaat taaaataggg
      541 agctttgtta tgccattaac ctcagatatt agatcacatt catttaatct tggggtggag
      601 gttgttcgtg cccgaattgt agccaatggg cgcggagata ttacagtcgg tggtgaaact
      661 gtcagtattg tgtatgattc tactaatggg cgcttttcat ccagtggcgg taatggcgga
      721 ttgctttctg agttattgct tttgggattt aatagtggtc ctcgagccct tggtgagaga
      781 atgctaagta tgctttcgga ctcaggtgaa gcacaatcgc aagagagtat tcagaacaaa
      841 atatctcaat gtaagttttc tgtttgtcca gagagacttc agtgcccgct tgaggctatt
      901 caatgtccaa ttacactgga gcagcctgaa aaaggtattt ttgtgaagaa ttcagatggt
      961 tcagatgtat gtactttatt tgatgccgct gcattt
//
LOCUS       DAZGJW010000498          995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZGJW010000498 DAZGJW010000000
VERSION     DAZGJW010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.503"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..264
                     /locus_tag="ACHKIK_005670"
     CDS             <1..264
                     /locus_tag="ACHKIK_005670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO9086562.1"
                     /translation="ILKKIMQRLCGCGKHDDREDGELLTAQLRLGPADILESDENGII
                     PEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            377..951
                     /locus_tag="ACHKIK_005671"
                     /pseudo
     CDS             377..951
                     /locus_tag="ACHKIK_005671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131658.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
BASE COUNT          288 a          171 c          252 g          284 t
ORIGIN      
        1 atattaaaaa aaattatgca gcgtctgtgc ggttgcggaa agcatgatga ccgtgaagac
       61 ggggagttac ttacagcaca gctgcgactg ggaccggcag acattctgga gtcagatgag
      121 aatggcatta tcccggagca ggacagggta atcacgcagg tggtgatact ggatgcggat
      181 aaaaagcaga tacagtgtgt ggtaagaccg ctgcaaatcc tgcgtgctga cgggacgtgg
      241 gaaaatattg gcgggatgaa gtaacccgac agcttcacaa aaccggagtc cggctccggt
      301 ttttgttgtc atgtaaggca gatgtttgtt aaagctattt aagtctggag tttaaattaa
      361 aatagggagt tttataatgc cgttaaattc ggagattaga tcaagctcat gtttaatgga
      421 ttgaatgtcc ttcgagctca agtagcatct agcggtcgag gggagtttac attaggtaat
      481 gagactgtca gcattgtatt taatgaaacc gatgggcgtt ttctatccag cggcagtagt
      541 gggggattgc ttactgagtt attcctttat gggtttaata acggccctga agctcttcgc
      601 gataggatgc tcagtatgct ttcggactca ggtgaagcac aatcgcaaga gagtattcag
      661 gacaaaatat ctcaatttaa gtttcctgtt agttcaggaa atttccagtg cccgccagag
      721 tctattcagt gtccaattac actagagaga cccgaagaag gagtgtttgt caaaaattca
      781 gatagttcgg cagtatgctg cttatttgat tttgatgcat tttctcgttt agctagtgaa
      841 ggctcatatc atccactgac ccgagaacca ataacggcat caatgattat aagtcctgat
      901 aaatgtgttt atgatcctat caagggaaac ttcattataa aagatagtta aaatattttt
      961 ctctgagaga gtattggctg tcagtaattt gtaaa
//
LOCUS       DAZGJW010000499          984 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZGJW010000499 DAZGJW010000000
VERSION     DAZGJW010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.504"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..158
                     /locus_tag="ACHKIK_005672"
     CDS             <1..158
                     /locus_tag="ACHKIK_005672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9086563.1"
                     /translation="RPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLS
                     SDMTMSR"
     gene            466..582
                     /locus_tag="ACHKIK_005673"
     CDS             466..582
                     /locus_tag="ACHKIK_005673"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086564.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            801..>984
                     /locus_tag="ACHKIK_005674"
     CDS             801..>984
                     /locus_tag="ACHKIK_005674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086565.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNL"
BASE COUNT          311 a          181 c          213 g          279 t
ORIGIN      
        1 ccaggccggt acgtcttggt agtggttctg tgggctggcg ttcatcagct attgacgaat
       61 ggcttacttt acatactgta cctgccagat cagcagtcaa aggtgttaat gacgaagtga
      121 gacatacgct ttcttcagat atgaccatga gcagataagt ttttgtcatc acgtgatcac
      181 ctcaggagga aaaagaagca taagaacaat ttagccagaa gtttaattga taaacagaat
      241 ttccagcatt cattgttgaa agagcagcta ttgtcgctat tgtagcggta attgctgcta
      301 taaccgaaac gtattacgaa aaaggaagca tttatgtctt acatcaaacc ggatatgccg
      361 tattcagtat atgcgtgacc atcaatgata gggaaggcaa taattaaagc tcaatacaat
      421 aaacaagtac ctctgactct ggttgcaatg tcagtattaa cagcgatgtc aattgcctgt
      481 cagaatcagg ttgacgtatg cagtcccgga aatttacgtg gggccgttaa tatttacact
      541 atggttctgg agaaagggaa aagaccgggg gaaaggtatt agtgaaataa tttccggagg
      601 tattaagaat gagaaatatg aatgatattt cgtggtgaat aatacagtct tcactgaatt
      661 aaccttatcg gtttattaaa catcagatct acaaatgctc tgtatttctc agagcatcag
      721 gtctgaatag cagctatttt cgctattgta gcgggatttg ctgctatttt actaacccat
      781 taatgaaaag agaggctttt atgtgcttac ttgcaccaga aaatccttat ccgatatatg
      841 cactaccacc gctggtgaga aatgcaataa ttgaaactca aaaaaataca caggcacctt
      901 tggctatggt ggcaacatcc gcattaacag cgatctcaat tgcctgtcag aatcagattg
      961 acgtgtgcag acctggaaat ttac
//
LOCUS       DAZGJW010000500          976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZGJW010000500 DAZGJW010000000
VERSION     DAZGJW010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.505"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..106)
                     /locus_tag="ACHKIK_005675"
     CDS             complement(<1..106)
                     /locus_tag="ACHKIK_005675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO9086566.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPV"
     gene            complement(213..557)
                     /locus_tag="ACHKIK_005676"
     CDS             complement(213..557)
                     /locus_tag="ACHKIK_005676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086567.1"
                     /translation="MSDLSMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEKIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(586..753)
                     /locus_tag="ACHKIK_005677"
     CDS             complement(586..753)
                     /locus_tag="ACHKIK_005677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086568.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(826..>976)
                     /locus_tag="ACHKIK_005678"
     CDS             complement(826..>976)
                     /locus_tag="ACHKIK_005678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086569.1"
                     /translation="ITKGYPKRDDSSRRIDVPYDGYEIKTITHPHFGDKPVKVFAIKV
                     NIGNE"
BASE COUNT          232 a          238 c          220 g          286 t
ORIGIN      
        1 taaccggagg agggaatatc ctgcattcgc gcacccatcg acgaactgtt tcaaggcttc
       61 ttgggcgtcg ctggcgtgca ttccactcct gaagtgtcaa gtacatcgca aagtctccgc
      121 aattacacgc aagaaaagcc gcattgatgc ggcgatggta ggtctggata tcattgagcc
      181 atgaacaggc ctcatcgagt gtgaggctgt ggttagtcct ttcgtaactc gctgattctc
      241 ctgtaagtct ctggtgcttt gtttccgtgt atcttcattt cagacttcaa cagagcaacg
      301 agggaatccc attcgttgag gatgcctttg aatgccggaa cgcgctttgc aaccttgtcg
      361 aatgaatctc tgatttctgg aatcttctca acaagtgcaa cgcatcgtcg gaagtctgct
      421 gcgtcatggg gagcgccgaa gtgatgacca tagatattct ttttcaggcc acatgcgatt
      481 gaggcaagag ttgcgctact gatgccaaca tcgccagtcg attgccattt caaaaccttc
      541 atagacaaat ctgacatttc ttgtctccaa taaaaaaaac ctccatcagg cggcttggtg
      601 ttctttcagt tcttcaattc gaatattggt tacgtctgca tgtgctatct gcgcccatat
      661 catccagtgg tcatagcagt cattgatgtt ctctgcttcg ataactctgt tgaatggttc
      721 tccattccat tcacctgtga ctcggaagtg catttatcat ctccataaaa caaaatccgc
      781 cgtagcgagt tcagataaaa gaaatccccg cgagtgcgag gattgttatt cattgccgat
      841 attcaccttt atcgcgaaca cctttaccgg tttatcaccg aagtgcggat gtgtgattgt
      901 cttgatttca tatccgtcat acggaacatc aattctgcgg ctggaatcgt cgcgcttcgg
      961 atatcccttt gtgata
//
LOCUS       DAZGJW010000501          970 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZGJW010000501 DAZGJW010000000
VERSION     DAZGJW010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.506"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..543
                     /gene="nleB"
                     /locus_tag="ACHKIK_005679"
     CDS             <1..543
                     /gene="nleB"
                     /locus_tag="ACHKIK_005679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFO9086570.1"
                     /translation="RDNYLLGLLREELKNIPEGKDSLIESYAEKREHTWFDFFRNLAM
                     LKAGTLFTETGKTGCHNISPCSGCIYLDADMIITDKLGVLYAPDGIAVHVDCNDEIKS
                     LENGAIVVNRSNHPALLAGLDIMKSKVDAHPYYDGLGKGIKRHFNYSSLHDYNAFCDF
                     IEFKHENIIPNTSMYTSSSW"
     gene            592..>970
                     /gene="nleE"
                     /locus_tag="ACHKIK_005680"
     CDS             592..>970
                     /gene="nleE"
                     /locus_tag="ACHKIK_005680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFO9086571.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALC"
BASE COUNT          317 a          148 c          205 g          300 t
ORIGIN      
        1 agggataatt atctgttagg cttattaaga gaagagttaa aaaatatccc agaaggtaag
       61 gactctttga ttgagtcata tgcagaaaaa agagaacata cttggtttga ttttttcagg
      121 aatttggcca tgttgaaggc tggaactttg tttacagaga cgggaaaaac tggatgccat
      181 aacatatcgc cctgtagcgg atgtatatat cttgatgccg acatgattat taccgataaa
      241 ttaggagtcc tgtatgctcc tgatggtatc gctgtgcatg tagattgtaa tgatgagata
      301 aaaagtcttg aaaatggtgc gatagttgtc aatcgtagta atcatccagc attacttgca
      361 ggcctcgata ttatgaagag taaagttgac gctcatccat attatgatgg tctaggaaag
      421 ggtatcaagc ggcattttaa ctattcatcg ttacacgatt ataatgcttt ttgtgatttt
      481 attgaattta agcatgaaaa tattataccg aataccagta tgtataccag cagttcatgg
      541 taagtcttcc ggttgtggtt gaaaaatatc gttctttagg tatagattca gatgattaat
      601 cctgttacta atactcaggg cgtgtcccct ataaatacta aatatgctga acatgtggtg
      661 aaaaatattt acccggaaat taaacatgat tactttaatg aatcacccaa tatatatgat
      721 aagaagtata tatccggtat aaccagagga gtagctgaac taaaacagga agaatttgtt
      781 aacgagaaag ccagacggtt ttcttatatg aagactatgt attctgtatg tccagaagcg
      841 tttgaaccta tttccagaaa tgaagccagt acaccggaag gaagctggct aacagttata
      901 tccggaaaac gcccaatggg gcagttttct gtagatagtt tatacaatcc tgatttacat
      961 gcattatgtg
//
LOCUS       DAZGJW010000502          959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZGJW010000502 DAZGJW010000000
VERSION     DAZGJW010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.507"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..882
                     /locus_tag="ACHKIK_005681"
     CDS             <1..882
                     /locus_tag="ACHKIK_005681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9086572.1"
                     /translation="FPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVM
                     RYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGSYKGQPVTQLAVM
                     LNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSK
                     QVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAM
                     ACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRE
                     RFISPFEYAKINNPLKQ"
BASE COUNT          267 a          229 c          219 g          244 t
ORIGIN      
        1 ttccccttta tcggcaatcg ggatatcaca acactcaata ccccggacct gcttatccct
       61 gttcgtgctg cagaagctaa acaaatttat gaaatcgcca gtcgtctgca gcaaagaata
      121 tctgccgtaa tgcgttatgc cgtacagtct ggcatcatca gatataatcc tgctctggat
      181 atggctggcg cattgactac ggtaaaacgc cagcatcgcc ccgctcttga tctttctcgc
      241 ctgcctgaac ttttgtcgcg tattggcagt tacaaggggc aacctgtcac ccagcttgcc
      301 gttatgctga atttactggt ttttattcgt tccagtgaac tcagatacgc ccgctggtct
      361 gaaattgata ttgacaatgc catgtggact attccagccg aacgcgaacc tctgccaggc
      421 gtaaaattct cacaccgggg atccaagatg cgaacaccac atcttgtgcc actcagcaaa
      481 caggttgtag ccatactggc agaacttcag acatgggctg gtgaaaatgg tctgatcttt
      541 acgggagcac atgacccacg taaaccaatc agtgaaaaca ccgtaaataa agccctgagg
      601 gttatggggt atgacacaac ccaggatgtc tgtggtcatg gattccgggc gatggcatgt
      661 agtgcgttaa tagaatcagg tttgtggtcc cgcgatgcag ttgaacgtca gatgagccat
      721 caggaacgta acggtgtacg tgctgcttac attcataaag cagaacatct ggaagaacgt
      781 cgactgatgc tacagtggtg ggccgatttt ctggatgcaa acagagaaag atttatcagt
      841 ccatttgaat atgcaaagat taataatcca ttaaaacagt aatcatcccg ggcaaatgcc
      901 cgggaaccta ctccaggatt attttctttg ttaaaaaaac agacaaaacg gtattaact
//
LOCUS       DAZGJW010000503          957 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZGJW010000503 DAZGJW010000000
VERSION     DAZGJW010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.508"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(60..599)
                     /locus_tag="ACHKIK_005682"
                     /pseudo
     CDS             complement(60..599)
                     /locus_tag="ACHKIK_005682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
BASE COUNT          278 a          232 c          193 g          254 t
ORIGIN      
        1 tggacaccac tttgtctaat tcgtcagatt ctgaccagac ggttcaggct gtacgcttac
       61 actgaacgcc atttaccaca aagctgattc ttaattgccg cagtcgtggc atcattaaaa
      121 atgaatttat acctcacagg ttcttccgca ctgtttttct gaagtacttc cagttgtctg
      181 agggtttctg actcatccgc gcctttgcgg agttctttat tcagttttga caacaatgct
      241 tttttctgag cctttaaaag ctcttccttg tttttccaga cagccagttc acttttgtat
      301 ctggaatata actgcatctc ctgctggtac aggggcttca ttaataattt gtccactgcg
      361 gtttttcgtt ctccggactc ggcaagcgtc aggaaaaaaa gagagaccgg cccccgcagt
      421 gtattcagtc tgcagacatc aatcagattc tggcagacaa gtgaaacagc gcccagggca
      481 gacgctgcca caagcccaag gggagcctgc gtaatttgct gagcttcaat aacggcatca
      541 cgaatgagtg gaggcagcgc actcagggga aactcatcac aaggctgacc gaatgacata
      601 gtaacctcac tacacacaga tataaaagca ctgcaattct ggctattaat agcaaaaata
      661 gcagtcaaat caatatgagg taaaattttt tgttgattta atggttaaat aataaccacg
      721 gtgtattttg ctgctgaaga aagacaaaat gaatctgtgt gtcccctcct tcccttcgga
      781 gcacatccac tattcaaatt ttacacaacc acgtacgaac aaaaagcatc atcccgggta
      841 ttgcagatac cggtttcctg ccgggaagaa tcataaacag aacaccggga ataacgcctt
      901 caggaatcac cccccctcct gaagaagaat aaaataaaga agactacagt tcctgca
//
LOCUS       DAZGJW010000504          954 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZGJW010000504 DAZGJW010000000
VERSION     DAZGJW010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.509"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..860)
                     /locus_tag="ACHKIK_005683"
     CDS             complement(<1..860)
                     /locus_tag="ACHKIK_005683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO9086573.1"
                     /translation="MAVKISGVLKDGTGKPVENCTIQLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMSEDDVRPEA
                     LRRFELMVEEVARHAEEAKKNAGEAETSARNAGISASQAEEGAANADTSAGEASESAR
                     QAAESAAAAKQSEEASSSSASAAAQKASESSQSAAEAELSRKTAESAAGNAARDATTA
                     TEKARESAESAQSAEQSRIAAEEAVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERGD
                     TGPAGATGERG"
BASE COUNT          150 a          328 c          230 g          246 t
ORIGIN      
        1 cccctttcac cggttgcccc tgccgggccg gtgtcgccac gctctccttt atctcccttc
       61 ggcccctgag gacccgcggg acccggttcc ccctttggcc cgggaggccc caccacggtg
      121 gggattcggt ttacggcctc ttccgccgct atcctgcttt gttccgctga ctgtgcgctt
      181 tctgctgact cccgggcttt ttctgttgcg gtcgttgcat ccctggctgc attaccggct
      241 gcactttctg ccgtctttct tgacaattca gcttctgctg cactttgtga tgactcactg
      301 gctttttgag cggccgcaga ggccgaggac gaggacgctt cctctgactg ctttgcagcg
      361 gctgcacttt ctgccgcctg ccgggctgac tccgatgcct cccctgctga agtgtcagca
      421 tttgcagcgc cctcttctgc ctgactggct gatatgccgg cattcctcgc tgacgtctcc
      481 gcctctccgg cattcttctt cgcctcctct gcgtgacgcg ccacctcttc caccatcagt
      541 tcaaaacggc gcagtgcctc cggccggacg tcatcctccg acatggcacc gagaaaatca
      601 ttcagcgtcc ccggttgaga atcttcatac acggtgatgg tcccggcatg tgacggcggg
      661 aagccctcca ccaacagaat gacgctgtac tgaccgtact caacgtccat gctgtaacga
      721 ccggcttcat ccggattttc agaggccacc gtattcacca ccaccgtgct gctggtccgt
      781 ctggctttca gttgaatggt gcagttctct accggttttc ctgtgccgtc tttcagtaca
      841 cctgaaatct ttactgccat attcacccca caaaaaagcc cgcctgaacc ggcgggctgt
      901 cataacactg tgttacctgg ctaatcagaa cttataaccg acacccacga tgaa
//
LOCUS       DAZGJW010000505          940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZGJW010000505 DAZGJW010000000
VERSION     DAZGJW010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.510"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            64..153
                     /locus_tag="ACHKIK_005684"
     CDS             64..153
                     /locus_tag="ACHKIK_005684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086574.1"
                     /translation="MPINLTSYLWVQGQKVVPAVVFSNFTLVD"
     gene            173..>940
                     /gene="nleL"
                     /locus_tag="ACHKIK_005685"
     CDS             173..>940
                     /gene="nleL"
                     /locus_tag="ACHKIK_005685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO9086575.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLENCSFKNSILNEC"
BASE COUNT          284 a          151 c          204 g          301 t
ORIGIN      
        1 gtcatgtccg gggaatattt gttagataaa aaagaggaga taattcaata gggagttaaa
       61 ttaatgccga taaatctgac atcttatttg tgggtacagg gacagaaagt tgtcccggca
      121 gttgtgtttt ctaattttac tttagtggac taagtaaaaa ggagtgagat aaatgctgcc
      181 cactacaaat atctctgtaa attctggagt aatatctttt gaaagtcctg tagattcacc
      241 atctaacgag gatgttgaag ttgccctcga aaagtggtgc gctgagggag aatttagcga
      301 aaatcgtcat gaggttgcat caaaaatact tgatgttata agtactaatg gagagacttt
      361 atcaatcagt gagccaataa caacattacc agacttgctt ccaggttctc tgaaagaact
      421 ggttttgaat ggatgtacag agcttaaatc aataaactgc ttacccccca acttatcttc
      481 attaagtatg gttggatgct catcattaga ggttataaat tgcagcatac ctgaaaatgt
      541 cattaattta tctttatgcc attgtagttc tttgaaacat atagaaggtt cctttcctga
      601 ggcactcaga aattccgtat atttaaatgg ctgtaattca ttaaatgaat cgcaatgtca
      661 attccttgca tatgatgtca gtcaaggccg tgcctgcctg agcaaagctg agcttactgc
      721 tgacttaatt tggttgtcgg ctaaccgaac gggtgaagag tctgctgaag aattgaatta
      781 ctctggatgt gacttgtcag gtctaagtct tgtagggctg aatttatcat cagtaaattt
      841 ttctggagca gtgcttgatg atacagatct caggatgagt gatttgtctc aggctgtatt
      901 ggaaaactgt tcttttaaaa actcgatttt gaatgaatgt
//
LOCUS       DAZGJW010000506          936 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZGJW010000506 DAZGJW010000000
VERSION     DAZGJW010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.511"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            75..860
                     /locus_tag="ACHKIK_005686"
     CDS             75..860
                     /locus_tag="ACHKIK_005686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086576.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          309 a          187 c          201 g          239 t
ORIGIN      
        1 tcaagtggag tatataatta aaaatcgtta gctaaacatt accatgatta tgacaggcga
       61 gggagtgtcc aattatgaat tcaaaaaagc tttgttgcat atgtgtgtta ttctcgctgc
      121 ttgcaggatg tgcctctgaa tcttctattg atgaaaagaa gaaaaaagct caagtcacac
      181 aaagtaatat taataaaaac actccccagc aactgacaga caaagattta ttcggtaatg
      241 aaaccactct ggccgtatcc gaagaggata ttcaagctgc gcttgatgga gatgagttcc
      301 gcgttcccct taattctcct gtaatacttg ttcaatccgg caaccgcgca ccggaaacca
      361 ttatgcagga ggagatgcga aaatattata ctgtttccac attctctggt atcccggaca
      421 ggcaaaagcc tctgacttgt aacaaaaaca aagataaaaa tgagaacgag gatgttgcca
      481 gtgctgagaa tatgaactgg atgcaggcac tgcgttttgt ggctgcaaaa gggcatcaga
      541 aagcgattat tgttttccag gatacgttgc agacaggaaa atatgactct gcgctgaaat
      601 caacagtatg gtccgactat aaaaatgaaa aactcaccga cgctatctcc ctgcgctacc
      661 tggtacgttt cacgctggtg gatgtggcaa caggtgagtg ggctacctgg tcgccggtga
      721 attatgaata taaagtgctt ccaccattgc ccgacaagaa tgaagccagt acgactgata
      781 tgacagagca gcaaatcatg caacttaagc aaaagaccta taaagcgatg gtaaaagatt
      841 tggttaaccg ctatcaataa taaattatat ctgccgccag gaatttctgg cggcaataat
      901 acaaaattct ttgcataaga aatttttctt gacaaa
//
LOCUS       DAZGJW010000507          935 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZGJW010000507 DAZGJW010000000
VERSION     DAZGJW010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.512"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..224
                     /locus_tag="ACHKIK_005687"
     CDS             <1..224
                     /locus_tag="ACHKIK_005687"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086577.1"
                     /translation="QRYDDSGVICTGGPGTLSGIATTKNSKSKGNGAGGVGGNRLIFR
                     YYRPVSAEGGSYLSGLRIPLDSITDWRNT"
     gene            complement(337..533)
                     /locus_tag="ACHKIK_005688"
                     /pseudo
     CDS             complement(337..533)
                     /locus_tag="ACHKIK_005688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            709..>935
                     /locus_tag="ACHKIK_005689"
     CDS             709..>935
                     /locus_tag="ACHKIK_005689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9086578.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPT"
BASE COUNT          247 a          249 c          234 g          205 t
ORIGIN      
        1 agcagcgcta cgatgactcc ggtgttatct gcaccggagg accgggaaca ttaagcggta
       61 tagcgacaac aaagaattcg aaatcaaaag ggaatggtgc cggaggcgtg ggcggtaata
      121 ggctgatttt caggtattac cggcctgtat ctgctgaagg gggctcgtat ttgtcggggc
      181 tgcgtattcc gctcgattcg atcacggatt ggcggaatac ctaactgtga ttgattcagg
      241 taccattttg cagggatccg gtgcgttact gtctgacaca acaaacgcag cgggtcgctc
      301 tgctccgccg attcctgata atccctcccc agtaacctac tgcaacagta caaatacact
      361 gacgcctgaa acggtaacca gaatcagcac cagcagtaac tcagtaaaca gagcagacga
      421 tcggtcttcc ggtaataacc ggaaggaacg acagcgccac aatacaggca acacactgta
      481 ttatgagtaa tcgaattacc gcaatccccg catttctttt tgcgtatcac gatagctccc
      541 tcgccacgcc ttatccgtaa ccggtttttt acattaaaaa aaacaacgcc gggaattcaa
      601 tcgtcagtcc ggagacgacc gttcgggtta tcacagagag cctgagcagg ggggctgcgc
      661 attttttacg atgtggctat tccgtatgaa ccatacggag atataaccat gacctacaaa
      721 tacaacccct tctggcagca acgtattcgt gagacggtgc ggcacgcact gaatgttcat
      781 ccccgcctga cggcattgcg ggttgacctg cgtttcccgg atgtaccggc agcaacggac
      841 gcagctgtga tatcccgctt catcaatgcc ctgaaagccc gaatcgacgc ttaccagaaa
      901 cgtaagcatc gggaaggtaa acgcgtgcat cccac
//
LOCUS       DAZGJW010000508          931 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZGJW010000508 DAZGJW010000000
VERSION     DAZGJW010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.513"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..117
                     /gene="gpW"
                     /locus_tag="ACHKIK_005690"
     CDS             <1..117
                     /gene="gpW"
                     /locus_tag="ACHKIK_005690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO9086579.1"
                     /translation="RRIEYTAASLDELNRAINDAESVLGTTRRRRRPLGVRL"
     gene            114..>931
                     /locus_tag="ACHKIK_005691"
     CDS             114..>931
                     /locus_tag="ACHKIK_005691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9086580.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKA"
BASE COUNT          189 a          224 c          321 g          197 t
ORIGIN      
        1 cgcagaattg aatatacggc ggcttctctg gatgagctta accgggcgat caatgatgcg
       61 gagtcggtac tggggacaac ccggcgtcgc cgtcgtccgc tgggagtgag gttatgaaac
      121 gaacgcctgt cctgattgat gtgaacggcg ttccgcttcg tgagagtctc agctacaacg
      181 ggggcggtgc aggatttggc gggcaaatgg ctgagtggtt gccaccggcg cagagtgccg
      241 atgcggccct gctgcccgcg ttgcgtctgg ggaatgcccg ggcagatgat ctggtgcgca
      301 ataacggaat agcggctaat gcggtggctc tgcataagga tcacattgtc gggcatatgt
      361 ttctgatcag ctaccgtccg aactggcgct ggctggggat gcgggagacc gcagcaaaaa
      421 gctttgtcga tgaggtggag gcggcctggt cggaatacgc cgaagggatg tctggcgaga
      481 tcgacgtgga aggaaaacgc acgttcacgg aatttatccg tgaaggtgtg ggcgttcatg
      541 cgtttaacgg cgaaatcttt gtgcagccgg tctgggatac ggaaaccacg cagttattcc
      601 gtacgcgttt taaagccgtg agtccgaaac gggtggacac gccaggacac ggtatgggga
      661 accgttttct gcgggccggg gtggaggtcg atcgatatgg ccgtgccgtt gcgtaccata
      721 tctgtgagga tgattttccg ttctccggga gtggacgatg ggaacggatc ccgcgtgaac
      781 ttcccaccgg gcgtccggcc atgctgcata ttttcgagcc ggtggaggac gggcagaccc
      841 gtggggccaa ccagttttac agcgtcatgg aacggctgaa gatgctcgat tccctgcagg
      901 caacacagct tcagtcggcc atagtgaagg c
//
LOCUS       DAZGJW010000509          923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZGJW010000509 DAZGJW010000000
VERSION     DAZGJW010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.514"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(96..789)
                     /locus_tag="ACHKIK_005692"
                     /pseudo
     CDS             complement(96..789)
                     /locus_tag="ACHKIK_005692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          243 a          194 c          209 g          277 t
ORIGIN      
        1 gcattattgt tattttactt tcaccttatc gctgtcgatt ctcattatca ttcattggta
       61 aaaaacagca aaagaggccc tggtaatgac tccagtcagt agtacagtgt tttcgacaaa
      121 tgaaccaatg gcttttcgtg aatttccagg gagcgtgaaa agcaaatcgt tttccgggct
      181 ggtcgtttga tgtgagtacg aagtgtcaga ttgttgcgct caatgcgccg ggtaaatatc
      241 ttgccgacaa gatgcttatc ctgtggcatt tctctggtat aactgctccg gttgtctctg
      301 gttatcatgc ctgtggagaa tggcttcagg agttccggca actcacgaca tgtttcatca
      361 gtgcgaggac caaaagtgta agccagcaca cctccagatt tggtgttata cgcataccag
      421 tgccactgct gacgagcttt attttcgaca aaactccatt gttcatcaag ttcgcagata
      481 agcgtcacat cggcactggc gactggagat gtggttactt tgcgtggtga gcgttttata
      541 aagtgagaat gacggtgttg atatccacct tcagcgttct ggagctatcg cgaaccccga
      601 ctccgttatg aaccatttca acaatttgct ctttaatgcc tggttttcgg gtttcgtaga
      661 tgtaatgcag ttaaaataca cacgtttgca ggattagcac tggaaacgct catgatctga
      721 ggtgctgtga tcataatggt acactttata ggaattacag cggggagaat gtactgtaac
      781 tgttgccata tggtctccag ataccaatag aataccacat taatctatcg tcagaaggca
      841 tcaccggggc cttctgacat aatctgttaa tacgtgttct attaccctgt tcagaaaata
      901 ttgattcaaa aatgaaaacc agt
//
LOCUS       DAZGJW010000510          922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZGJW010000510 DAZGJW010000000
VERSION     DAZGJW010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.515"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(158..894)
                     /locus_tag="ACHKIK_005693"
                     /pseudo
     CDS             complement(158..894)
                     /locus_tag="ACHKIK_005693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          253 a          186 c          210 g          273 t
ORIGIN      
        1 aactgtcgtt acagtttgta ttccccgggt aaatttgtct gcgtaaaaaa atgggttgag
       61 aaaaaagaca ataaataatt aaaaatcaat atgatagaaa acgacagcat tagtgttatt
      121 ttacttttcc cttatcgctg tcgattctca ttatcattca tggcaaaaaa acagcaaaag
      181 aggccctggt aatgactcct gtcagtagta catgtgtttt cgataaatga accaatggct
      241 tttcgtgaat ttccagggag cgtaaaaagc aaatcgtttt ccgggcaggt cgtttgatgt
      301 gagtacgaag tgtcagattg ttgcgctcaa tgcgccgggt aaatatcttg ccgacaagat
      361 gcttatcctg tggcatttct ctggtataac tgctccggtt gtctctggtt atcatgcctg
      421 cggagaatgg cttcaggaat tccggcaact cacggcatgt ttcatcagtg cgaggaccaa
      481 aagtgtaagc cagcacaccg tcagctttgg tcttatacgc gtaccagtgc cactgctgac
      541 gagctttgtt ttcgacaaaa ctccattgtt catcaagttc acagataagt gccacatcga
      601 cactggcggc tggagatatg gttactttac gtggtgagcg ttttttaaag tgagaatgac
      661 ggtgttgata tccaccttca gcgttctgga gctatcgcga accccggctc cgttatgaac
      721 catttcaaca atttgctctt taatgcctgg ttttcgggtt tcgtagatgt aattcagttg
      781 aaatacacgt ttgcaggatt agcactggaa acgctcatga tctgaggtgc tgtgatcata
      841 acggtacact ttataggaat tacagcgggg agaatatact gtaactgttg ccatatggtc
      901 tccagatacc aatagaatac aa
//
LOCUS       DAZGJW010000511          921 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZGJW010000511 DAZGJW010000000
VERSION     DAZGJW010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.516"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(61..759)
                     /locus_tag="ACHKIK_005694"
     CDS             complement(61..759)
                     /locus_tag="ACHKIK_005694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9086581.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNAEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDNPTTDIRKDRCSKCMRGCEMRGMA
                     VNFGGFLSINKLSQ"
     gene            complement(759..>921)
                     /locus_tag="ACHKIK_005695"
     CDS             complement(759..>921)
                     /locus_tag="ACHKIK_005695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9086582.1"
                     /translation="WLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYSDF
                     SCTIEQVVN"
BASE COUNT          207 a          323 c          210 g          181 t
ORIGIN      
        1 ggcgcacacc gccgggcatg cgccagaatc gctgattcag tctgtgtcat aaaacaggat
       61 ttactgcgaa agtttattga tggaaaggaa accgccaaaa ttaaccgcca tgccgcgcat
      121 ctcacacccg cgcatgcact tgctgcatct gtccttacgg atatccgtgg tggggttgtc
      181 gaactcatcc gccacagccc cgcccgtgta accacactca tcagagcggt aggtccacat
      241 acaggtattc gccagcatga tgcgaccggg aaacagcgcc ccatccgtct cggtcggtgt
      301 agccagcaca aacgaggccg tcatggctgt cagctgcgac atctgctcca ccacccagcg
      361 gtcactcagc tcctgctccg ggtccgcctc cgcattgccc gcaacgaaat tcaccgcatc
      421 cagaaaacgg gcatacaccc ggcggcggac caccgtggcc cccaccagac tctgcaggtc
      481 ctccgccatc ccggtgacaa ggccaaacag attggacacc gtcagcgacg ggcgggcact
      541 gctgccccgg ccgttcatct caaagccgct gccgtcaatc gggtatgcct catacttacg
      601 cccctgccag gtgaccggct cccctttttc attcagctca ttacagaaaa aataccgctc
      661 accaccctgt accgtcaggt cgatttccca gagtaccacc cgcggtgact gctctgactt
      721 aaccgactcg ttcagacttt cttcgcgaat atcctgcatc agttcaccac ctgctcaatc
      781 gtacaactga aatcactgta cctggcgtta tctgtgacgc tccactcccg gcacaccacc
      841 ctcaccgtcc ggttatgttt cggcggtcgc cacaaaaagg cacggtaacc accatgccac
      901 gataaaaact cttccagcca g
//
LOCUS       DAZGJW010000512          916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZGJW010000512 DAZGJW010000000
VERSION     DAZGJW010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.517"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..93)
                     /locus_tag="ACHKIK_005696"
     CDS             complement(<1..93)
                     /locus_tag="ACHKIK_005696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086583.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNS"
     gene            complement(77..>916)
                     /locus_tag="ACHKIK_005697"
     CDS             complement(77..>916)
                     /locus_tag="ACHKIK_005697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO9086584.1"
                     /translation="WLAQCGLTVERLAAQIEPVYLPERKIHLYHCDHRGLPLALISED
                     GNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRHRYYDPLQGRYIT
                     PDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAVSGVPGLIAANSITNS
                     AYQFGYDMDAIVGGAHNGAADAMRHCYLMCRMTKTFGSTIADVIGKNHEAAGDRQGQP
                     AKERIMDLKNNTVGIACGDFSAKCSDACIEKYNTGQLFGLDGIKADNPIKAKQGSSDA
                     SNY"
BASE COUNT          239 a          248 c          175 g          254 t
ORIGIN      
        1 agaatttgaa ttgttatcaa acccaaggta aacaacaaat aaacaaaggg taattaaaaa
       61 aatgacaagt ttgcctctaa taatttgaag catctgaact cccttgcttt gcttttattg
      121 gattatctgc ttttatacca tctaacccga agagttgccc agtgttatat ttttcaatac
      181 atgcatcgct acatttggca gaaaaatcgc cacaagcaat accgacagtg ttatttttaa
      241 gatccatgat tctttcttta gctggctgac cttgtctatc ccctgccgcc tcatgatttt
      301 tacctatcac gtcagctatt gttgatccaa atgtcttagt cattcgacac atcaagtaac
      361 aatgtctcat tgcatcggcg gccccattat gagctccgcc aacaatagca tccatatcat
      421 aaccgaactg gtaagcactg ttagtaatgc tgtttgcagc aatcaatcca ggtacaccag
      481 atacggcata aattagtcca cctgatgtca tattctcaat cgcatctaac cccattgggt
      541 ctatcacttg tatgggattc aacggatact gatacatatt ccatccacct ctcaacccaa
      601 tcgggtccgg ggtgatatac cgcccctgca acggatcgta gtaccggtga cggttatagt
      661 acagccccga ctccttatca tactgctgcc ccggcagccg gtacggctgg tgcaggtgat
      721 gcgggttctc ctcattaagc tggttgcccc attcatcata ttccgcgctc cacgccgtat
      781 tgccgtcttc actgataagc gccagcggca ggccccggtg gtcacagtga taaagatgta
      841 tttttcgctc aggcaaataa accggctcta tctgtgccgc cagccgctcc accgtcagcc
      901 cgcactgcgc aagcca
//
LOCUS       DAZGJW010000513          900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZGJW010000513 DAZGJW010000000
VERSION     DAZGJW010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.518"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..808
                     /locus_tag="ACHKIK_005698"
     CDS             <1..808
                     /locus_tag="ACHKIK_005698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFO9086585.1"
                     /translation="RMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGK
                     RFINGAVIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQ
                     DLSPSGPLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEY
                     KKLFALPDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNAL
                     LNILESQFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
BASE COUNT          248 a          181 c          217 g          254 t
ORIGIN      
        1 cagaatgacg ttatccctca tggtgcaacc cggtcttttt gaccgatata tggagcgaaa
       61 aggttctgtt gccagggaca gtggttttct tgcccgttgt cttatcagta aacctgcaac
      121 cacgcagggg aaacgcttta ttaacggagc tgtgattccg ggaggatcat taacggcatt
      181 tcatgaacga ttaatggaac tggccagagg cagtattgaa aaatccagtg aggatgaacg
      241 gtattgtctg catttttcgc ctgaagcgca aaaaatattt attgagcact ataatgttct
      301 ggagcaggat ttatcccctt ccggaccctt aagcccgttt cggggacatg tttcaaaaaa
      361 aacagaaaac attgcaagaa ttgcagcgtt atttcagtat ttcagctatg gtgaagggaa
      421 aatctctgcc gatatcatga cgtctgctgt cgtaataagt tcgtggtaca cagatgaata
      481 taaaaaatta tttgcgttac ctgatgaatc tgaactgcag cagaaggacg ccgaggagct
      541 gttcgactgg ctcattgagg agtgccgcgg agaatgcccg ccccgtgtca ggaaaaatta
      601 tattctccag tgtggcccgg gccggttcag aaacaggaaa aaactgaacg cattgctgaa
      661 cattcttgaa tctcagttca ggctttctgt cgtgccggaa ggaaaaacca tgtatgttct
      721 gcttccccag atagccagtt taaagttgtc tgatgtctct ggtattttta cctcgggtta
      781 tcactacaat aagctgagag cgaagtaacc ggtgagcagt ttttctccat tatgcttcag
      841 acttagatga ttgattatta tggggatttc atcaccgata ttcagatgta tgaatatcgg
//
LOCUS       DAZGJW010000514          895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZGJW010000514 DAZGJW010000000
VERSION     DAZGJW010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.519"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..153)
                     /locus_tag="ACHKIK_005699"
     CDS             complement(<1..153)
                     /locus_tag="ACHKIK_005699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO9086586.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGASSLSVPQ
                     QQVQFDA"
     gene            complement(143..>895)
                     /locus_tag="ACHKIK_005700"
     CDS             complement(143..>895)
                     /locus_tag="ACHKIK_005700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9086587.1"
                     /translation="SELDTEKAFEYIAGAPQGQKDNPLINILDKFSTWYDTNNVTLGG
                     VKIPHLFPGDDLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSA
                     RASANESWRYFMGRRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSR
                     AEWIGAGRMAIDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAERQK
                     AGLSRPVWIAQAYEQQIAESRRPEEETTPRET"
BASE COUNT          207 a          270 c          217 g          201 t
ORIGIN      
        1 agcatcaaac tgtacctgct gttgtggtac cgaaagactt gatgccccca tctcgcgccc
       61 gagcgcgcaa aagaaaaccc gcgcataggc gggctccaga agcagcggtt cattgaatgc
      121 tgcggcaata atgtgtgaaa gattacgtct cacgtggtgt tgtctcctct tccggcctgc
      181 gactctccgc tatctgctgc tcatacgcct gcgctatcca caccggacgt gagagtccgg
      241 ctttttgtcg ctcagcagat tccctgacct gctggcggaa aatgtcctga taatcctcgc
      301 ccatcagcgc cagctctttc tcatacgtgc tcagtccggc ctcaatgcgc atcactgatt
      361 cctggacttc cttgagcccg tcaatggcca ttcttccggc tccaatccac tcagcccgtg
      421 accaggctga tcgcgcctga taaaaatcaa aacgcgcccg tggcggacga ataatccccc
      481 gaagaagtgc ctcttccagc cagcaggaaa acatctgcgt ggccagccgg gacgcaataa
      541 attttcgtcg tcccataaaa tagcgccacg actcattggc ggaggcgcgg gcacttgaat
      601 aactgacctt cgagtaatcc cgggacaact gttcgtagga aacgccaaga ccggcggcga
      661 tataccgcag cagcgcctgt tcaagcgccg aaaatccatt gtctgaatcc tgcgcagtct
      721 gcagtttcag atcatcaccg gggaaaaggt gcggaatttt gacaccgccc agcgtcacgt
      781 tattcgtgtc ataccaggtg gagaacttat ccagaatatt aataagcgga ttatccttct
      841 gcccctgcgg cgcaccggcg atatattcaa aggccttttc ggtatcaagt tcact
//
LOCUS       DAZGJW010000515          888 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZGJW010000515 DAZGJW010000000
VERSION     DAZGJW010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.520"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..358)
                     /locus_tag="ACHKIK_005701"
     CDS             complement(<1..358)
                     /locus_tag="ACHKIK_005701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024231387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6543 domain-containing protein"
                     /protein_id="HFO9086588.1"
                     /translation="MNTQWQQKYLLEYNDLVSKFPSPEKVTSDYIKHKFKTDLPWFSR
                     VDPDKTYFIQFSQNRSNSRSYTGWDHLGKYKTDALTLTQAAIINIGYRFEVFDEANAT
                     AGIYTTNNADLFDETNE"
BASE COUNT          251 a          166 c          170 g          301 t
ORIGIN      
        1 cttcgtttgt ttcgtcaaat aaatcagcat tgtttgttgt gtaaattcct gcagttgcat
       61 tggcctcatc aaagacctca aaacgatatc cgatattaat aattgctgct tgtgtaagtg
      121 ttagtgcatc tgttttatat tttcccaggt gatcccagcc tgtgtaagat ctactgttgc
      181 tccggttttg agaaaactgg ataaaataag tcttgtcagg atcaacccta ctaaaccagg
      241 ggaggtcagt tttgaattta tgcttaatat agtcgctggt tactttctct ggtgacggaa
      301 attttgacac cagatcatta tattccagaa ggtatttttg ttgccattgg gtattcataa
      361 tattattcac gctttagata agtatatgaa tgtgttgtta cttccccaaa atatttatca
      421 tattcacatg taaaattgtt ttgttgtttg aaatgaatta caaatgtagt gttgacttat
      481 gtaattgcag ttgttttgtg ttaatttatt gaaatttaag taaatatata caatccggag
      541 gctgattatg ttaatatctc cccggattat acgaacgata acggaatccc taaatattct
      601 tcaaaaatca acaaatcgct gtataggaca ttttggcgta cagctccttt atggctgccg
      661 caaaaagcat ggaccgttat ggacctcagt taatccggga ttgtttgtgc cagactattc
      721 gagtaggcag cctggcggct gcggcttgtc atggcctgaa attaccgtta taaaaacaga
      781 caatatcatt gtctttcagg tagttatatg tcccgttcag ctaaaccccg taaacgaaaa
      841 cctgcccctc aaagaagcaa acttccccgc tatgtcgtga agcttcac
//
LOCUS       DAZGJW010000516          884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZGJW010000516 DAZGJW010000000
VERSION     DAZGJW010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.522"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            341..556
                     /gene="essD"
                     /locus_tag="ACHKIK_005702"
     CDS             341..556
                     /gene="essD"
                     /locus_tag="ACHKIK_005702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO9086589.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            556..>884
                     /gene="rrrD"
                     /locus_tag="ACHKIK_005703"
     CDS             556..>884
                     /gene="rrrD"
                     /locus_tag="ACHKIK_005703"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO9086590.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVG"
BASE COUNT          233 a          181 c          207 g          263 t
ORIGIN      
        1 aatttaaatc ggctaaaaat aacgctggaa attatttgtt ggttatttgt tgagatttgt
       61 ttatgtattt gtagtggtgt tttcaatact cggtagcatt ctcgcaaata tcatttagtg
      121 gtttacgtac gtaaaaaatt ggttatgctg ttaagagtgg ttacttcgtc acacagctta
      181 aacccgccgt cgagcgggtt tttccatttt ttgagtctcg atattagctg ataacccaat
      241 acctgagtta ttcactgact ccgagtctgt tacgtttctg cttttttgcg atacgttgta
      301 ttccctcaat ttacacccgc tttgtctgcg aggtggggtt atgaaatcca tggataagtt
      361 aacaacgggt gtcgcctatg gcacctcagc aggtagtgcc ggttactggt ttttacagct
      421 gctcgataaa gtcacgccct cacagtgggc agcaataggt gtgctgggta gcctggtatt
      481 tggcctgctg acgtacctga caaaccttta tttcaagatt aaagaagata agcgcaaggc
      541 tgcgagaggt gaataatgcc tccatcatta cgaaaagccg ttgctgctgc tattggtggc
      601 ggagcaattg ctatagcatc agtgttaatc actggcccaa gtggtaacga tggtctggaa
      661 ggtgtcagct acataccata caaagatatt gttggtgtat ggactgtatg tcacgggcat
      721 acaggaaaag acatcatgct cggtaaaacg tataccaaag cagaatgcaa agcactcttg
      781 aataaagacc ttgccactgt cgccagacaa attaacccgt acatcaacgt cgatataccg
      841 gaaacaacgc gcggcgctct ttactcgttc gtttacaacg tggg
//
LOCUS       DAZGJW010000517          881 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZGJW010000517 DAZGJW010000000
VERSION     DAZGJW010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.523"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            26..208
                     /locus_tag="ACHKIK_005704"
     CDS             26..208
                     /locus_tag="ACHKIK_005704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086591.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNTEDN
                     DEAASDYALKINENSD"
     gene            complement(437..511)
                     /locus_tag="ACHKIK_005705"
                     /pseudo
     CDS             complement(437..511)
                     /locus_tag="ACHKIK_005705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(508..>881)
                     /locus_tag="ACHKIK_005706"
     CDS             complement(508..>881)
                     /locus_tag="ACHKIK_005706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9086592.1"
                     /translation="KLRMVELASQPGASVARIAREHDINDNLLFKWLRLWQNEGRISR
                     RLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQTSVSAGSCKVEFRHGNMT
                     LENPSPELLTLLIRELTGRGR"
BASE COUNT          246 a          189 c          206 g          240 t
ORIGIN      
        1 tgcccccccc aaaggagatg atttcatgta taattgtggt gaatcatatt cagaatacga
       61 catatcatta tctgacgatg aaaactatga cgatgactat tatcgtgaac gcccgtgtcc
      121 gggagaagtt ttcggctatg ataatactga ggataatgat gaggcggcat ctgattatgc
      181 attaaaaata aatgaaaact ctgattaata cgggggtagc tgataataag acaatcaatg
      241 acagatatta atgaataaac ataatcaggc gctcaatact tggtttaatc tgggcgcctg
      301 attaaaatct gaggaaacat ataataattt tcatcaaaat agtatttatg tactaactaa
      361 gctggatttg aacctctatt tctgggtatt tgttatcact taatccatta ctgctcctcc
      421 ttcagcatat gtcgtatccg ttgaaggatt tacgcatatc ggtaacgtcg gcaacgagcc
      481 agatgcgggt acctgacggg agtgagatca tcgtcccctc ccggtcagtt cacggatcaa
      541 cagcgtgagc agctctggcg atggattttc cagcgtcatg ttaccgtgac ggaactccac
      601 cttgcaggag ccggcactga cactggtctg agtggaagtg gataaagacg gcgcaatggc
      661 cgccaccggt tctttctgct catccggcgt tatttctaca ggtaataatt caacgccagt
      721 gtcagaagag gtcgttacct gaagacgccg tgatatacgc ccttcgttct gccagagcct
      781 gagccatttg aacaggaggt tatcattgat atcgtgttcc cgggcaatac gggcaacaga
      841 ggctcctggt tgtgatgcca gttccaccat acgaagtttg a
//
LOCUS       DAZGJW010000518          877 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZGJW010000518 DAZGJW010000000
VERSION     DAZGJW010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.524"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..756
                     /gene="yeeO"
                     /locus_tag="ACHKIK_005707"
     CDS             <1..756
                     /gene="yeeO"
                     /locus_tag="ACHKIK_005707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO9086593.1"
                     /translation="LAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVESVLFTS
                     GRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVGQIAQAE
                     IQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALFMPIWSA
                     SWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMFADWAVR
                     AVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
BASE COUNT          183 a          180 c          246 g          268 t
ORIGIN      
        1 ctggcgattg gttttaatcc tgcgctaagg atttcgttaa agagctattt taaaccgctg
       61 aattttagca ttatctggga agtcatgggg attggtattc cagcgagtgt cgaatcagtg
      121 ttatttacca gtggtcggtt attaacccaa atgttcgttg ccgggatggg gaccagtgtt
      181 attgccggaa attttatcgc gttttcaatt gcggctctta tcaacttacc cggaagtgcg
      241 ctcggctctg cttctacgat cattacaggc cgaaggttag gggtagggca gatagcgcaa
      301 gcagagattc agttgcggca tgtcttctgg ctgtccactc ttggattaac ggccatcgcc
      361 tggctaacgg ctccctttgc cggggttatg gcatcgtttt acacccagga tccacaggtt
      421 aaacatgtcg ttgtgattct gatttggcta aatgctttat ttatgcctat ttggtccgcc
      481 tcatgggtgc tacccgctgg atttaagggg gctcgtgacg cccgttacgc tatgtgggtt
      541 tctatgttga gcatgtgggg ttgccgggtt gtcgtcggtt atgtgctggg catcatgctt
      601 ggctggggtg tggttggtgt ctggatggga atgtttgccg actgggctgt gcgggccgtg
      661 ctgttttact ggcgaatggt tactggacgt tggctatgga aataccctcg acccgaaccg
      721 caaaagtgtg aaaaaaagcc agttgtgtcg gaataaacaa caaaatgcag attatttcag
      781 caaacgattt caaatttaaa aaacaggctt tgacattgtg ggtgggcatc gctaatattc
      841 gcctcgttct cacgattcct ctgtagttca gtcggta
//
LOCUS       DAZGJW010000519          872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZGJW010000519 DAZGJW010000000
VERSION     DAZGJW010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.525"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..70
                     /locus_tag="ACHKIK_005708"
     CDS             <1..70
                     /locus_tag="ACHKIK_005708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086594.1"
                     /translation="DEITKKKSPVAAGDSKQIDMQF"
     gene            103..>872
                     /locus_tag="ACHKIK_005709"
     CDS             103..>872
                     /locus_tag="ACHKIK_005709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO9086595.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLIEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ
                     GGMFGPNKNISEWCIPQNEGGSPKMREIPQNEGKSPKTRDKTSLKLGDCYPSKQGDTK
                     DTITKEKRKDYSSENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMV
                     KTIAPSARKPNFAGWANDIRLMRERDGRNHRDMCVLFR"
BASE COUNT          290 a          191 c          209 g          182 t
ORIGIN      
        1 tgatgaaatc acaaagaaaa aatccccggt ggccgccggg gactctaagc aaattgatat
       61 gcaattctga gggaattact ggatcaatcc acaggagtca ttatgacaaa tacagcaaaa
      121 atactcaact tcggcagagg taactttgcc gaacaggagc gtaatgtggc agatctcgat
      181 gatggttacg ccagactatc aaatatgctg attgaggctt attcaggcgc agatctgacc
      241 aagcgacagt ttaaagtgct gcttgccatt ctgcgtaaaa cctatgggtg gaataaacca
      301 atggacagaa tcaccgattc tcaacttagc gagattacaa agttacctgt caaacggtgc
      361 aatgaagcca agttagaact cgtcagaatg aatattatca agcagcaagg cggcatgttt
      421 ggaccaaata aaaacatctc agaatggtgc atccctcaaa acgagggagg ttcccctaaa
      481 atgagggaga tccctcaaaa cgagggaaaa tcccctaaaa cgagggataa aacatccctc
      541 aaattagggg attgctatcc ctcaaaacag ggggacacaa aagacactat tacaaaagaa
      601 aaaagaaaag attattcgtc cgagaattct ggcgaatcct ctgaccagcc agaaaacgat
      661 ctttctgtgg ttaaaccgga tgctgcaatt cagagcggca gcaagtgggg aacagcagaa
      721 gacctgaccg ccgcagagtg gatgtttgac atggtgaaga ccatcgcacc atcagccaga
      781 aaaccgaatt ttgcagggtg ggctaacgat atccgcctga tgcgtgaacg tgacggacgt
      841 aaccaccgcg atatgtgtgt gcttttccgc tg
//
LOCUS       DAZGJW010000520          871 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZGJW010000520 DAZGJW010000000
VERSION     DAZGJW010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.526"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>871
                     /locus_tag="ACHKIK_005710"
     CDS             <1..>871
                     /locus_tag="ACHKIK_005710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO9086596.1"
                     /translation="EDIEDFGETHLDFLKQYGDFENDIPVHDTIARVVSCISPAKFHE
                     CFINWMRDCHSSDDKDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIRTD
                     EKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNK
                     AFEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCV
                     AVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNED
                     DCKIRRGNAAELFS"
BASE COUNT          289 a          155 c          207 g          220 t
ORIGIN      
        1 ggaagatata gaggatttcg gggaaacaca tctcgatttc ttgaagcaat atggtgattt
       61 tgaaaatgat attcctgttc acgatactat tgccagagtt gtatcctgta tcagtcctgc
      121 aaaatttcat gagtgcttta ttaactggat gcgtgactgc cattcttcag atgataaaga
      181 cgtcatcgca attgatggaa aaacgctccg gcactcttat gacaagagtc gccgcagggg
      241 agcgattcat gtcattagtg cgttctcaac aatgcacagt ctggtcatcg gacagatcag
      301 gacggatgag aaatctaatg agattacagc tatcccagaa cttcttaaca tgctggatat
      361 taaaggaaaa atcatcacaa ctgatgcgat gggttgccag aaagatattg cagagaagat
      421 acaaaaacag ggaggtgatt atttattcgc ggtaaaagga aaccaggggc ggctaaataa
      481 agcctttgag gaaaaatttc cgctgaaaga attaaataat ccagagcatg acagttacgc
      541 aattagtgaa aagagtcacg gcagagaaga aatccgtctt catattgttt gcgatgtccc
      601 tgatgaactt attgatttca cgtttgaatg gaaagggctg aagaaattat gcgtggcagt
      661 ctcctttcgg tccataatag cagaacaaaa gaaagagcca gaaatgacgg tcagatatta
      721 tatcagttct gctgatttaa ccgctgagaa attcgccaca gcgatccgaa atcactggca
      781 cgtggagaat aagctgcact ggcgtctgga cgtggtaatg aatgaagacg actgcaaaat
      841 aagaagagga aatgcagcag aattattttc a
//
LOCUS       DAZGJW010000521          865 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZGJW010000521 DAZGJW010000000
VERSION     DAZGJW010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.527"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(5..703)
                     /locus_tag="ACHKIK_005711"
     CDS             complement(5..703)
                     /locus_tag="ACHKIK_005711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9086597.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(703..>865)
                     /locus_tag="ACHKIK_005712"
     CDS             complement(703..>865)
                     /locus_tag="ACHKIK_005712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9086598.1"
                     /translation="WLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYSDF
                     SCTIEQVVN"
BASE COUNT          191 a          305 c          193 g          176 t
ORIGIN      
        1 ggatttactg cgaaagtttg ttaatggaaa ggaaaccgcc aaaattagcc accatgccgc
       61 gcatctcaca cccgcgcatg cacttgctgc atctgtcctt acggatatcc gtggtggggt
      121 tgtcgaactc atccgccacc gcaggaccgt tatacccgca ttcatctccc cggtaatccc
      181 acatacaggt gttcgccagc atgatgcgac cgggaaacag cgccccatcc gtctcggtcg
      241 gtgtagccag cacaaacgag gccgtcatgg ctgtcagctg cgacatctgc tccaccaccc
      301 agcggtcact cagctcctgc tccgggtccg cctccggatt gcccgcaacg aaattcaccg
      361 catccagaaa acgggcatac acccggcggc ggaccaccgt ggcccccacc agactctgca
      421 ggtcctccgc catcccggtg acaaggccaa acagattgga caccgtcagt gacgggcggg
      481 cactgctgcc cttcccgttc atctcaaaac cgctgccgtc aatcgggtat gcctgatatt
      541 gccgcccctg ccaggtgacc gcctcccctt tttcattcag ctcattacag aaaaaatacc
      601 gctcaccacc ctgcaccgtc aggtcgattt cccagagtac cacccgcggt gactgctctg
      661 atttaaccga ctcgttcaga ctttcttcat gaatgtcctg catcagttca ccacctgctc
      721 aatcgtacaa ctgaaatcac tgtaccgggc gttatccgtg acgctccact cccggcacac
      781 caccctcacc gtccggttat gtttcggcgg tcgccacaaa aaggcacggt aaccaccatg
      841 ccacgataaa aactcttcca gccag
//
LOCUS       DAZGJW010000522          864 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZGJW010000522 DAZGJW010000000
VERSION     DAZGJW010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.528"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>864
                     /locus_tag="ACHKIK_005713"
     CDS             <1..>864
                     /locus_tag="ACHKIK_005713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9086599.1"
                     /translation="KGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQSIL
                     VNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTRTI
                     TSANIDRLRVTFGVQSLVQTTSKGDRNPTSVRLLIQLQRNGNWVTEKDVTINGKTTSQ
                     FLASVILDNLPPRPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIVGL
                     QVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWD
                     MLTHPRYGMGKR"
BASE COUNT          212 a          237 c          268 g          147 t
ORIGIN      
        1 aaaggtggcg gcagggcgca cacacctcgt gaggcgaagg acaatctcaa atccacgcag
       61 atgatgagcg tgattgacgc cattggtgaa gggccgattg aaggtccggt gaaggggctg
      121 cagagtatcc tggtgaacaa aaccccgctg acggacacgg acggtaatcc tgtgatacat
      181 ggtgtgaccg cggtctggcg cgccggggag caggagcaga caccacctga aggctttgag
      241 tcctccgggg cggaaaccgc actgggcgtg gaagtgacga aggcaaagcc ggtgacgcgc
      301 accattacat ccgcgaacat tgaccgcctg cgggtcacct tcggggtgca gtcactggtg
      361 cagaccacgt caaagggtga ccgaaacccg acatccgtcc gactgctgat tcagttacag
      421 cgtaacggta actgggtgac ggaaaaggat gtcaccatta acggcaagac cacctcgcag
      481 tttctggcgt cggtgattct ggataatctg cctccccggc cctttaacat ccggatggtc
      541 cgggagacag cggacagcac ctcggaccag ctgcagaata agacgctctg gtcgtcatac
      601 accgaaatca tcgatgtgaa acagtgctac ccgaacacgg cgattgtggg gctgcaggtg
      661 gatgcggagc agttcggcgg ccagcagatg acggtgaact accatatccg tggtcgcatc
      721 attcaggtac cgtcaaacta tgacccggaa aaacgcacgt acagcggtat ctgggacggc
      781 agcctgaaac cggcatacag caacaacccg gcctggtgcc tgtgggacat gctgactcac
      841 ccgcgctacg gcatgggaaa acgt
//
LOCUS       DAZGJW010000523          857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZGJW010000523 DAZGJW010000000
VERSION     DAZGJW010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.529"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>857
                     /locus_tag="ACHKIK_005714"
     CDS             <1..>857
                     /locus_tag="ACHKIK_005714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO9086600.1"
                     /translation="HIRTITHRVTDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQY
                     KAEDMLREAIGGLASRPEGFVVYTTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLP
                     VIFEHPPEMVESGAHLLMENLAMVNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSK
                     HANVEIGLALRSDRWAGADFWEQQGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVI
                     GRDRETREWLGWGHAWAHETAVVRRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYV
                     RRIHEAELL"
BASE COUNT          174 a          201 c          295 g          187 t
ORIGIN      
        1 catatccgga ccatcaccca tcgggtgacg gacaccaccc tgaaggtggt ggcagccgat
       61 ccgaatacgg tgtccggtat caagtccgtg gggactctga ttgatgaact gtggctgttt
      121 ggcaagcagt acaaggcgga agacatgcta cgtgaagcca tcggcgggct tgcctcccgt
      181 ccggaagggt ttgtggtgta tacgaccacc cagtcgaatg agccgccagc cggggtgttc
      241 agacagaaac tgcagtacgc ccgggatgtc cgtgacggca aaattcatga tccgcatttt
      301 ctgccggtga tatttgaaca ccctcctgaa atggtggaaa gcggggctca cctgctgatg
      361 gaaaacctcg ccatggtcaa tccgaatctc ggttattcgg tggatgaggc ttttctgtac
      421 cgggagtacc gtaaagcccg ggaggctggt gaggaagcat ttcgtggctt catgtcaaaa
      481 catgccaatg tggaaattgg tcttgccctg cgttctgacc gctgggcggg tgcggatttc
      541 tgggagcagc agggcaggcg cgtcagcctg gacgatatcc tgcagcgcgc tgatgtggtg
      601 acggtgggga ttgacggcgg gggcctggat gatctgctgg gaatgtacgt gattggccgt
      661 gacagggaaa cccgcgaatg gctgggctgg ggccatgcct gggcgcatga aaccgcggtg
      721 gtcagacgga agagtgaggc atcccgtttt caggattttg tggcctgtgg agacatgacg
      781 attgtccgtc gggtcgggga tgacacggcg gaagtggcgg agtatgtgcg tcgtattcat
      841 gaggctgagt tactgga
//
LOCUS       DAZGJW010000524          857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZGJW010000524 DAZGJW010000000
VERSION     DAZGJW010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.530"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>857
                     /locus_tag="ACHKIK_005715"
     CDS             <1..>857
                     /locus_tag="ACHKIK_005715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9086601.1"
                     /translation="VAAARGTEMQIAAEARLAATQERLNRNIAARTAAQNALNSTTAV
                     GSRLMTGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALTIDEIAHKTPSMS
                     LPEASDNEGRTRAALAEQNRLIDEQASRVKSLQEKIAGYQYVLANPGWTTGDGFMINH
                     LTSVKTVTEGLSQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRRVALIRQQAAEQN
                     KVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLDDKQQSALTKTER
                     ELALSRLKG"
BASE COUNT          217 a          210 c          280 g          150 t
ORIGIN      
        1 gtggcggcag cccgcgggac ggagatgcag attgctgcag aagcccgtct ggcggccaca
       61 caggaacgcc tgaacagaaa tattgctgcc agaaccgcag cccagaatgc gctgaacagt
      121 acaacggcgg tgggctcacg tctgatgact ggtgcgctgg gactggttgg tggcgtaccc
      181 ggactggtga tgctgggggc agcagcatgg tatacgctgt accagaatca ggagcaggcc
      241 agggagtcag cgcgccagta tgcactgacg atagatgaaa tcgcgcataa aacgccgtca
      301 atgtctttgc ctgaagcctc agataatgaa ggacgaacac gggcggcgct ggcagagcag
      361 aaccggctga ttgatgaaca ggccagccgg gtgaaatccc tgcaggaaaa aatcgcagga
      421 tatcagtatg ttctggcgaa cccgggctgg acgaccggtg acggattcat gataaaccat
      481 ctgacatcgg tgaagaccgt aacggaaggg ctttctcagg caacagagca gcttgccgtt
      541 gagcagtccc gtctggcaca gatgcaggaa aaagcgcagt ccattcagga tgtgcttgcc
      601 gggctggaag accgtcgtgt ggcgttaatt cgtcagcagg cggcagagca gaataaggtg
      661 taccagtcca tgctggttat gaacggtcag catacggaat tcaaccgtct gctggggctg
      721 gggaatgaac tgcttcagca gcggcaggga ctggtgaatg tgccgttacg gctgccacag
      781 gccactctgg atgataaaca gcagagtgcc ctgacaaaaa cagagcgtga gctggccctg
      841 tccagactga aagggga
//
LOCUS       DAZGJW010000525          853 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZGJW010000525 DAZGJW010000000
VERSION     DAZGJW010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.531"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(393..>853)
                     /locus_tag="ACHKIK_005716"
     CDS             complement(393..>853)
                     /locus_tag="ACHKIK_005716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO9086602.1"
                     /translation="ILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKT
                     EAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRA
                     QAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDTVVSVARAVWNWIFF"
BASE COUNT          206 a          225 c          228 g          194 t
ORIGIN      
        1 ggtgttcaga tgtcgtttca tcagcttttc cttacagagg gtgaataaaa aagccggtac
       61 ccacaaatgt ggataccggc aggtacaaaa cagcatgctc agataaagtg gtttttctcg
      121 gacataaatg cttcccctct ctcatccgcc agagaagtat cagtccgttg tgtgcaattc
      181 cgttctgcat gatgaagaga ttcagctgcc ttttcgcaga ccattaacca ctataaacga
      241 tcgatagcaa cgatcgatac ctgttttatc gatcgtttta ttctattaac gacaaggcgg
      301 tcacgcaaga gaaacaggag gagtatgagg ggtttgtgtc atgttttcag ttggttaacg
      361 taatgttatc aggcatttcc ctggcagact gttcagaaga aaatccagtt ccatacagcg
      421 cgggcaacag agaccaccgt gtcacggacg gcacgcagaa ccgtacgggc aacagaggca
      481 acacagacgc tctccgccgt gtcaaatatc cggtccaccg caccggtaaa ctgttcacgg
      541 gcctgagcgc ggacagattc cgtgcgcagc tcgtcctgca ggcgggtcat caggggactg
      601 gcggcatggt cgggaagtgc tgtcattagc gcactgacca gcgtatccag ttcccagccg
      661 gtgcgggccg atacggcaac aaccggatgt acgggccgga acagacggaa taccgcctcc
      721 gttttctccc gaatgttctg tgcctgtgcg ggagaaggct gaatgccggc catatcccat
      781 tcatggcagg gctccgtttt gtcggcctgc gtcaccacaa acagcacctg ctgatgtccc
      841 cggtgcagga tgt
//
LOCUS       DAZGJW010000526          846 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZGJW010000526 DAZGJW010000000
VERSION     DAZGJW010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.532"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            119..>846
                     /locus_tag="ACHKIK_005717"
     CDS             119..>846
                     /locus_tag="ACHKIK_005717"
                     /inference="COORDINATES: protein motif:HMM:TIGR01586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HFO9086603.1"
                     /translation="MDSSLVDPVTWESIGYTGGDSYIKSPISSPTLHDISIRAKYRAL
                     EWGNFYGHNARLWYETVIKYSSSEPKYHPQMLLSPNEGRCIGLSELYILADTKEKYNT
                     LQENLDLISSLYQQDISENAHLSESDHRFLKNTVSQIEYYQQHGNNKLLQSEALERIR
                     LTDFNTASVARYLKDKNINNILITTEHHSLVVSVFDDVVRVTDPNFGYADFSSLEQSL
                     EFIERSVIISPDIKEIYTGKKGGVA"
BASE COUNT          291 a          149 c          149 g          257 t
ORIGIN      
        1 aacgggcaac agatctttta tcgactgtat ctcatctatc gtgcataata aactgcccag
       61 cgtatcagaa atcctcttta aggaaaaaga atatagactt ccatcaatat attctataat
      121 ggattcatca ttagttgatc ccgtaacatg ggaaagtata ggctatacag gaggcgatag
      181 ttatattaaa tcaccaatat catctccgac attacatgat atttctattc gggcaaaata
      241 tcgagcgctg gaatggggaa acttttatgg gcacaatgct agattatggt atgaaaccgt
      301 gataaaatac tccagcagtg agcccaaata ccatccacag atgttgcttt cacccaatga
      361 aggccgttgt atcggtttaa gtgagttata tatccttgct gatactaaag aaaaatataa
      421 tacgttgcaa gagaaccttg atttaataag ctcattatat caacaagata tatctgaaaa
      481 tgcacatctt tcagaaagtg atcatcgatt tttgaaaaac actgtatcac aaattgaata
      541 ttatcaacag catggtaata ataaattatt acagtccgag gcgcttgaaa gaatacggtt
      601 aactgatttt aatacagcca gtgttgctcg ttatttaaaa gacaaaaaca taaataatat
      661 tctgataacc acagaacatc acagtcttgt agtatccgtc ttcgatgatg tggttcgtgt
      721 tacagatcct aacttcggat atgctgattt ttcatctctg gaacaatcat tagaatttat
      781 tgagcgttct gttatcattt ctccagatat aaaagaaatc tatacgggta agaaaggtgg
      841 tgtcgc
//
LOCUS       DAZGJW010000527          839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZGJW010000527 DAZGJW010000000
VERSION     DAZGJW010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.533"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..796
                     /locus_tag="ACHKIK_005718"
     CDS             <1..796
                     /locus_tag="ACHKIK_005718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO9086604.1"
                     /translation="FFIAMFVTPIALILFVFDYGYKKSPQEYSLSFIKQLPNTVNLSS
                     LTYNKEDDFLYATQNSPAQLLKIARSGDIIGRAPLPFISDAETIEHIQGNIFAAVDEK
                     TSELFFFTVTKDMHISLRNKIQLEKFNKKNRGFEGLAWKAEGRMLFVAKERRPTGMFA
                     YQLSPDLLSAKQVTIPEELNDIHVKDISGLDFNNESLMILSDESRKLLKFNLTEMSFV
                     EMMDLTKGNHSLTSDLLQPEGIVTLSDESIYVASEPDILAKFVPNK"
BASE COUNT          271 a          168 c          156 g          244 t
ORIGIN      
        1 attttttatt gcaatgtttg tcacaccaat tgcattaata ttatttgtat ttgattatgg
       61 ttacaaaaaa tcaccacagg aatactcact ttcatttata aaacaattac caaatacggt
      121 gaacttatca tctcttacgt ataataaaga ggatgatttc ctgtatgcca cccagaatag
      181 ccctgcgcag ctacttaaaa tagccagaag tggcgacatc atcggccgag ccccgctgcc
      241 ttttatctct gatgccgaaa ccattgagca catacaaggg aacatttttg ctgcagtaga
      301 cgaaaagact tctgaactat tcttttttac agttacaaaa gatatgcata tctccttgcg
      361 aaataaaatt cagttagaaa aattcaataa aaaaaataga gggtttgaag gactggcatg
      421 gaaagcagaa ggccggatgc tgtttgtcgc taaagaacga cgccctacag gcatgtttgc
      481 ctatcagctt tctccagatc tgctgagtgc aaaacaagta acgattccag aggaactaaa
      541 tgatatacat gttaaagata tatccggcct ggactttaat aatgaaagtc tgatgattct
      601 gtccgatgaa tcgcgtaagt tactcaagtt taacctgaca gaaatgtctt tcgttgagat
      661 gatggacctg acaaaaggaa atcattctct gaccagtgat ttgctccaac ctgaaggcat
      721 tgtaactctg tctgatgaaa gcatttatgt cgctagtgaa cctgatatcc tcgctaaatt
      781 tgtccctaat aaataagtct gtcactagcc ttagtattga gcgaccaaat ctggcaatc
//
LOCUS       DAZGJW010000528          836 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZGJW010000528 DAZGJW010000000
VERSION     DAZGJW010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.534"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..170
                     /locus_tag="ACHKIK_005719"
                     /pseudo
     CDS             1..170
                     /locus_tag="ACHKIK_005719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
     gene            complement(189..821)
                     /locus_tag="ACHKIK_005720"
                     /pseudo
     CDS             complement(189..821)
                     /locus_tag="ACHKIK_005720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
BASE COUNT          259 a          171 c          127 g          279 t
ORIGIN      
        1 attttatata accatttttc acgttgtgga gtaacgtggc aatgcctggg gacttataat
       61 gcgggttttg caatggataa ccaaaaaaag agacagcaat atgccccaaa atatacattg
      121 tatataccag gcttaatgaa ctagacaatc gtaaggcttc agcaaaatga gcctaaagcc
      181 tctttttttt atatagtgca aacacccata cgtaaagctg ttttgatcaa ctcgatacca
      241 ctgtgaacat ctaatttctt catgatattg agtctatgag tttcgactgt ttttctgctt
      301 aactgcaaga aattagcgat ttctttgttt gttttaccat cagctatgag ttgtaatatt
      361 tctgactcac gatttgtaag ttgattatca gaggaatacc tggaagatat tttttcacaa
      421 tccatatgta cactatcaaa ataagtgtaa ccattactaa tcgaatcaat agcacgattt
      481 agtttcgccg ggtgactggt tttccatacg catcctttcg cacctgcttc aagcaatttt
      541 attgaatcaa tatacggcat ctttgctaca aatgtaataa tatgagcatc tatcttatgc
      601 gatttaaccc attttacgag ttcggacccc atcattccac ctgcaaattc tcctaaaaaa
      661 aatatttagg ctgtagattt gttacactcc gctatagctt tcttcagatc tttatagccc
      721 ccaacaacct catataaatc aggataatgt ccgagaaatc caattatccc atcaatcata
      781 aacggctgct gatctgaaac tacaatttta atttttccca tatattcatc ctgagt
//
LOCUS       DAZGJW010000529          834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZGJW010000529 DAZGJW010000000
VERSION     DAZGJW010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.535"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..746
                     /locus_tag="ACHKIK_005721"
     CDS             <1..746
                     /locus_tag="ACHKIK_005721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFO9086605.1"
                     /translation="VSVINPALGKAFAQSEGLRNKTDTVDARMLAEFCRQKRPAAWEA
                     PHPLERALRALVVRHQALTDMHTQELNRTETAREVQRPSIDAHLLWLEAELKRLEKQI
                     KDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGLKDRFAHARQFAAFAGLTPRRY
                     ESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEWGRAFRDRLAANGKKGKVILGA
                     MMRKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT          204 a          205 c          267 g          158 t
ORIGIN      
        1 tagtgtcagt cattaatcct gcgctgggta aagctttcgc tcagagtgaa ggactgcgta
       61 acaagactga taccgtggat gcgcgcatgc tggcagagtt ctgtcgtcag aagcgccctg
      121 cagcctggga agcgcctcac ccgcttgaac gcgcgttgcg tgccctggta gtccgccacc
      181 aggcgctgac agatatgcac acgcaggaac tgaatcgcac tgaaacggcg cgggaagtcc
      241 agagaccgag cattgatgct caccttctgt ggcttgaagc agagctgaag cgtcttgaga
      301 agcagataaa agacctgaca gacgatgatc cggatatgaa acaccgcagg aaactgctgg
      361 aaagcatccc gggtatcgga gagaaaacat ctgcggtatt gctggcttat atcggtctga
      421 aggaccgctt cgcccatgcc agacagttcg ccgcttttgc gggtctgaca ccacggcgtt
      481 atgaatcagg tagcagtgtg agaggggcga gccggatgag taaggccgga catgtgtcgc
      541 ttcgcagggc gttgtatatg cccgcaatgg tagccaccag taagactgag tggggacggg
      601 cgttccgcga ccgtctggcg gctaatggca agaaaggaaa ggtgattctc ggcgcgatga
      661 tgcgcaagct ggcacaggtg gcgtatggag tgctgaagtc aggcgtgccg ttcgatgcgt
      721 cacggcataa tccggtagcg gcgtaaaaat cgcggaaggg atgaaaaaaa cagcgcctga
      781 cggcgctgtg tctggcatgc ctgcaatccg ggaaaccgga ccaggaaaaa actt
//
LOCUS       DAZGJW010000530          834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZGJW010000530 DAZGJW010000000
VERSION     DAZGJW010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.536"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(84..>834)
                     /locus_tag="ACHKIK_005722"
     CDS             complement(84..>834)
                     /locus_tag="ACHKIK_005722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086606.1"
                     /translation="PKGPIPWFGLTAQVVRNHGVELPIVKTENGWKLVGETPLTPDGP
                     KANYTEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDLDNPNTKYAVLTNAAANAL
                     GALGGFAVSRFASTDPMLSPHIGAMVGQAAGHAIQYNTPGLKPDTILWWAGATLGAAD
                     LNKAEFEVARFTDYPRIWWHAREGAIFPNKADIEHATGADIRAMEEGIPVGQRHPNPE
                     DVVIDIESNGLPHHNPSNHVDIFDIIQETRV"
BASE COUNT          226 a          194 c          157 g          257 t
ORIGIN      
        1 ttttagaaaa aaaatatatt aatccttatt aataatagct gccatccatt tcagctatta
       61 ttttaaaata aacaagttaa agcttagact cttgtttctt ggattatatc aaagatatca
      121 acatgatttg atggattatg atgtggtaag ccattgcttt cgatatcgat taccacatcc
      181 tctggatttg gatgccgctg tccaacaggg ataccttctt ccattgcgcg tatatcagca
      241 cctgtggcat gttcaatatc tgctttattg gggaaaatag ctccttctct tgcgtgccac
      301 catatacgag gatagtcagt gaatctagct acttcaaact cggccttgtt taaatcggca
      361 gcccccagtg tcgcaccagc ccaccataaa atagtgtctg gctttaatcc aggggtatta
      421 tactgtatgg catgccctgc tgcttgccca accattgcac cgatatgagg acttaacatt
      481 ggatctgtac tagcaaatct ggatactgca aatcccccta aagcaccaag cgcatttgcg
      541 gcagcattgg taagaacggc atatttagta ttagggttat ctaaatccca cttgaaatgt
      601 gcgccaaact ccagccctag agttgcctgt aatggagatg caccatattt aaaatctgct
      661 tctcccggtc tgataaccca ctcctccgtg taatttgctt tcggcccatc aggagtaagt
      721 ggtgtttctc caacaagctt ccatccattt tcagttttga ctataggaag ttctacacca
      781 tgattacgaa ccacttgagc tgttaatcca aaccatggta ttgggccttt tggc
//
LOCUS       DAZGJW010000531          833 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZGJW010000531 DAZGJW010000000
VERSION     DAZGJW010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.537"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            168..353
                     /locus_tag="ACHKIK_005723"
     CDS             168..353
                     /locus_tag="ACHKIK_005723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO9086607.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRTIYFSRTV
                     VRNKKVIGTFIKKHIFY"
     gene            594..>833
                     /locus_tag="ACHKIK_005724"
     CDS             594..>833
                     /locus_tag="ACHKIK_005724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-LEE encoded effector protein NleB"
                     /protein_id="HFO9086608.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYPLEPIDEKTP
                     ILFQWFEARPERYEKGEVPILNTKEHPYLSNIINAA"
BASE COUNT          276 a          153 c          150 g          254 t
ORIGIN      
        1 atctatcacg gcactctcag ttgagtatca caggcacatg tattttagcc ccctgagtct
       61 acagacaacc aatattactt aaataagtga taatcttaat gctgtttttt agaacagatg
      121 attgatattt ttgggccgga gaaacgcaga caggctttgt tgaataaatc gagcttttgc
      181 tgagtttaag gatcagatca ctcattttcc tgcaaaagct cattgggcga aacaatctga
      241 ctcagagaac ccggattaaa cgtctggccc gcagaaccat ctatttctca cgcactgtag
      301 tacgtaataa aaaagtcatc ggtactttta tcaaaaaaca tatattctat taattattgc
      361 catcaccttt ttcttattga caacattact ctgctgattg ttaaaatcta acctgtattc
      421 agggttacga ttctatacaa tagaacaggg aggggaaagg atgtcatcgt taatgagact
      481 ttttgttaat gtcaacaatg ctatgaattt attttagaaa aacaattaag ctgacgtagt
      541 gttccgtcta aatgacgggg cgatattaac atgattagaa caagaggaat tttatgttat
      601 cttcattaaa tgtccttcaa tccagcttca gaggaaagac agccttatca aatagtacac
      661 ttctccagaa agtttctttt gctggaaaag aatatcctct ggaacctatt gatgaaaaaa
      721 cccctattct ttttcagtgg tttgaagcaa ggccagagcg atacgaaaaa ggagaagtac
      781 caatattgaa taccaaagaa catccgtatt tgagcaatat tataaatgct gca
//
LOCUS       DAZGJW010000532          822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZGJW010000532 DAZGJW010000000
VERSION     DAZGJW010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.538"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..700
                     /gene="ydeO"
                     /locus_tag="ACHKIK_005725"
     CDS             <1..700
                     /gene="ydeO"
                     /locus_tag="ACHKIK_005725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO9086609.1"
                     /translation="DYAFSDGEILMVDNAVRTHFEPYERHFKEIGFTENTIKKYLQCT
                     NIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFAACKG
                     FITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTFSQI
                     LLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQSH
                     TGMNTGNTMSALAI"
BASE COUNT          251 a          165 c          167 g          239 t
ORIGIN      
        1 agattacgcc ttctctgacg gcgagatcct gatggtagat aacgctgttc gtacgcattt
       61 tgaaccttat gagcggcatt ttaaagagat cggatttact gaaaatacca ttaaaaaata
      121 tctacaatgc actaacatcc agacagtgac ggtgcctgtt cctgcgaagt ttttacgtgc
      181 ttcaaatgta ccgactggat tgcttaatga aatgattgct tatctcaact cggaagaacg
      241 caatcatcat aatttttcag aacttttgct tttttcttgc ctgtctattt ttgccgcatg
      301 caaaggtttc attacactat taactaacgg tgtgctatcc gtttccggaa aagtgagaaa
      361 tattgtcaac atgaagctgg cgcacccatg gaagctgaaa gatatttgtg actgcctgta
      421 catcagtgaa agcctgttga agaaaaaact aaagcaagag caaacgacat tctcacagat
      481 tctattagat gccagaatgc agcacgcaaa aaatttgata cgcgtagaag gttcagtcaa
      541 taaaattgcc gaacaatgtg gttatgccag tacatcttat tttatttatg cgttccgcaa
      601 acatttcggc aacagtccga agagagtttc taaggagtac cgttgtcaaa gtcacacggg
      661 tatgaatacg ggcaacacga tgagtgcttt agctatttga ttatttgcta acgagtagtc
      721 aaccacgcac gctacgtaag aattatatgg ggcagccatt ccctgccccg cgttgttttt
      781 aggcgatata tttattgaaa taaataagtg acatccatca ca
//
LOCUS       DAZGJW010000533          822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZGJW010000533 DAZGJW010000000
VERSION     DAZGJW010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.539"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            52..591
                     /locus_tag="ACHKIK_005726"
     CDS             52..591
                     /locus_tag="ACHKIK_005726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HFO9086610.1"
                     /translation="MNWHYEKNGVRYDNVTEEDIIERIRRGELNASTLVWQQGMLEWQ
                     PVSATSLAEILKQSEVPPALPGHRIPGGVVWTLAFAPLIGYILEMWTAGLNGMSFERA
                     YDAVSEGQYWFISLILNIALGYLDERKLRKSGVDTATFGWLAWLIPVYLWRRAKILGQ
                     KPAYFWVWLVMLILVLFTI"
BASE COUNT          220 a          146 c          205 g          251 t
ORIGIN      
        1 tgtttcagat gtcatatatt ttattaacta aatgattaac aggagaatca gatgaactgg
       61 cattatgaaa aaaatggtgt acgttatgat aacgtaacag aggaagatat tatcgaacgt
      121 atccggcgtg gtgaactgaa tgcatcaaca ttggtgtggc aacagggcat gctggaatgg
      181 cagccagttt cagccacatc actggcggaa atactgaaac aaagtgaagt tcccccggcc
      241 cttccgggtc accgcattcc cggaggcgtt gtctggacgc tggcatttgc ccctttgatt
      301 gggtacatac ttgagatgtg gactgccggg ctgaacggta tgtcatttga acgggcttat
      361 gatgcagtca gcgaaggcca gtactggttt atctctctga ttctgaatat tgctctggga
      421 tatctggatg aacgaaaact ccgtaaatct ggtgttgata ctgcaacatt tggctggctg
      481 gcatggttga tcccggttta tctgtggcgt cgggcaaaga tcctcgggca aaaacctgct
      541 tatttctggg tctggctggt tatgctgata ctggtgttat ttacgatctg atattctttt
      601 tcgcagagtt tgctaaataa tcaggttatt agtgttctta acaggtaaat gatgttcatc
      661 atggagcgga tatattttat ttgattcagg agacagtcag aaacatagta taaatatatc
      721 accatgttga aggtcatatc ttcaggtatg tgtttaataa acaagtcggg ggttaatcct
      781 gacctctcga gtggattata agtattgcag aaacagtttt ta
//
LOCUS       DAZGJW010000534          813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZGJW010000534 DAZGJW010000000
VERSION     DAZGJW010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.540"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            87..677
                     /locus_tag="ACHKIK_005727"
     CDS             87..677
                     /locus_tag="ACHKIK_005727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO9086611.1"
                     /translation="MSRIFAYCRISTLDQTIENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT          260 a          168 c          176 g          209 t
ORIGIN      
        1 gtacatacaa atatgcacaa aaataatcaa aattattttc tgagatgcat tatgatatga
       61 acaccaattt cgtatagagt ctcactatgt ctcgaatttt tgcttactgt cggatatcaa
      121 cgctggatca gaccatcgaa aatcaacgcc gggaaatcga aagtgcaggt tttaaaatca
      181 aacctcagca aataatcgaa gaacacatta gcggctcagc agcaaccagt gagcgtcctg
      241 gttttaaccg gttgcttgct cgcctgaaat gtggtgatca attgattgtg acaaaactgg
      301 atcgccttgg ttgtaatgca atggatatca ggaaaacagt ggaacaactg accgaaacag
      361 gtatcagagt gcattgctta gcattggggg gcattgacct gaccagtcca acaggaaaaa
      421 tgatgatgca cgtaatttca gcagtcgctg aatttgaacg agacctttta cttgaacgca
      481 ctcattccgg gatagtaaga gcccgcggcg cagggaaacg ttttggtcga ccacctgtgt
      541 taaatgaaga acagaaacag gtggtattcg aacgaattaa gtcaggtgta agtataagtg
      601 ccattgcccg ggaattcaaa acctcgcggc aaaccatttt aagagccaaa gcaaaacttc
      661 agacacctga catataaaaa ataatctcgg tgtgagatgc tttacgtctt ccaagccccc
      721 ttccttgccg taaatggaaa gatacatcta attatagaat ttatatgttt taccctacgg
      781 cagtgctggc cattcaatat cctgtgcagt tga
//
LOCUS       DAZGJW010000535          806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZGJW010000535 DAZGJW010000000
VERSION     DAZGJW010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.541"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            140..649
                     /locus_tag="ACHKIK_005728"
     CDS             140..649
                     /locus_tag="ACHKIK_005728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO9086612.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            621..>806
                     /locus_tag="ACHKIK_005729"
     CDS             621..>806
                     /locus_tag="ACHKIK_005729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086613.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMN"
BASE COUNT          241 a          161 c          202 g          202 t
ORIGIN      
        1 ggtttctctt caagttaact atatgaaaaa tataaaaaca ggtcttctgt gaaccggaca
       61 tgcacaaaaa atagatatgt aaaccggaca tgaccggttt tgttgtgatt gtgaggtgag
      121 agtttttgcg aggtgaggag tggctacgca gactgaagtt gccaggcatt taagtctgac
      181 cgatcgccag cttcgcagat tgcagaaatt gccgggtgcc ccgatatcaa ataagcgagg
      241 gcaactggat ctggatgcct ggcgcgattt ttacatatcg tatctgagga gaagtaaaaa
      301 cgatgtgcct gatggcgata gcgaagacga ctatgaggaa aaattgctta ttgccagatg
      361 ggaactgaca gcagaacagg ctgttacaca gcagttaaaa aatgaggtgt caaaaggaaa
      421 acttattgac accgggttct gtatttttgc cctcagtaag ctggcaatgg cgttatccag
      481 tacgcttgat tccatccctt tatccatgca acgacagttt cctgatttaa caccgcgcca
      541 tcttgaccat ctgaaaaccc ttattgcgaa gggggcaaat cagtgtgcgc gggcggggga
      601 taaattaccg gatttactcg atgaatatat cagagcaaca actgaataat atgatgagtg
      661 ctgtcacaac agcattacag cccctgataa gggcattgcc ggtgacgcca gttgaatggg
      721 ctgatcaaaa ttattatctg cctaaagaat cttcatatgg tgagggagaa tggaaaacgc
      781 tgccattcca gatcgccatc atgaac
//
LOCUS       DAZGJW010000536          801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZGJW010000536 DAZGJW010000000
VERSION     DAZGJW010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.542"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            39..407
                     /locus_tag="ACHKIK_005730"
     CDS             39..407
                     /locus_tag="ACHKIK_005730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFO9086614.1"
                     /translation="MSNIKKYIIDYDWKTSIEIEIDHDLMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDPDDMTIKAA"
     gene            480..644
                     /locus_tag="ACHKIK_005731"
     CDS             480..644
                     /locus_tag="ACHKIK_005731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO9086615.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNNYGYPD"
     gene            613..777
                     /gene="kil"
                     /locus_tag="ACHKIK_005732"
     CDS             613..777
                     /gene="kil"
                     /locus_tag="ACHKIK_005732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO9086616.1"
                     /translation="MDQTIMAIQTKFTIATFIGDEKMFREAVDAYKKWILIQKLRSSK
                     SIHYPPFLFS"
BASE COUNT          253 a          163 c          169 g          216 t
ORIGIN      
        1 aatgtgaggg cgattttttt ctgtgaggat atgaacagat gtcaaacatc aaaaaataca
       61 tcattgatta cgactggaaa acatcaatag aaattgaaat cgaccatgac ttaatgacag
      121 aggaaaaact tcaccagatt aataatttct ggtcagactc tgaataccga ctcaataaac
      181 acggctctgt attaaatgct gtattaatca tgctggcgca acatgctctg cttatagcaa
      241 tttcaagcga cttaaatgca tatggtgttg tgtgtgagtt cgactggaat gatggaaatg
      301 gtcaggaagg atggcctcca atggatggta gcgaaggaat aagaattacc gatatcgata
      361 catcaggaat atttgatcca gatgatatga ctatcaaagc cgcctgagcg cggcgttacc
      421 gcataccaat tacgcttcac tcgaggcgtt tttcgttatg tataaataag gagcacacca
      481 tgcaatatgc cattgcaggg tggcctgttg ctggctgccc ttccgaatct ttacttgaac
      541 gaattacccg taaattacgt gacggatgga aacgccttat cgacatactt aatcagccag
      601 gagtcccgaa aaatggatca aacaattatg gctatccaga ctaaattcac tatcgccact
      661 tttattggcg atgaaaagat gtttcgtgag gccgtcgacg cttataaaaa atggatatta
      721 atacagaaac tgagatcaag caaaagcatt cactaccccc ctttcctgtt ttcctaatca
      781 gcctggcatt tcgcgggcga t
//
LOCUS       DAZGJW010000537          800 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZGJW010000537 DAZGJW010000000
VERSION     DAZGJW010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.543"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>800)
                     /locus_tag="ACHKIK_005733"
     CDS             complement(<1..>800)
                     /locus_tag="ACHKIK_005733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086617.1"
                     /translation="IPTDSIGGEDIYFIHDPQGGWYPNRGNHLYAIDALCVNIHDALA
                     NSIFDGLENYHKFLYMAPEFLSTAGLNSESVVSKETFVLFIDKFKGHTDVNKGLYLFD
                     CCKIVSSIQECSKEVLQLQGEFYYTLNFEPLFFPNIEEEDGIRYVTSPVVTKLFALLG
                     FIYIRMYSLLDYITKLAIEIENLKTQFSSYVKLTSKNSQYGDKKKVSLNNYKGSLFEQ
                     CPFINEIESVRNHIIHDGLLDDMPKAYRVIRNNACIEKYLLFPDQTSEG"
BASE COUNT          252 a          145 c          135 g          268 t
ORIGIN      
        1 ccttctgatg tttggtcagg gaaaagcaaa tatttttcaa tacatgcgtt gttccggata
       61 actctgtatg cttttggcat atcgtctaaa agaccatcat gtatgatatg gtttcgtaca
      121 ctttctattt cattaataaa tgggcattgc tcaaacaaag atcctttata attatttaag
      181 ctcactttct ttttatcacc atattgagag ttcttggatg tgagtttaac ataagaagag
      241 aactgtgttt ttaaattttc aatttctata gctagttttg ttatgtagtc gagcaaactg
      301 tacatgcgga tataaataaa tccaagtaat gcaaagagct tagtcacgac tggagatgta
      361 acatatctta taccgtcctc ctcttcaatg ttagggaaaa acaagggttc aaaattcaac
      421 gtgtaataaa actccccttg tagttgcaac acttccttgc tgcattcctg tatgctactt
      481 acaattttac agcaatcaaa aaggtaaagg cctttattta cgtcagtatg ccctttgaat
      541 ttatcaataa ataaaacaaa tgtttctttt gaaactacgg attcagagtt caaacctgct
      601 gttgataaaa actcaggggc catataaagg aacttgtggt agttttctaa tccatcaaaa
      661 atgctattag ctagagcatc atgaatatta acgcataatg catcgatcgc ataaagatga
      721 ttccctctgt tcgggtacca tccaccttga ggatcatgta taaagtaaat gtcctcacct
      781 ccaattgagt cagttggtat
//
LOCUS       DAZGJW010000538          797 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZGJW010000538 DAZGJW010000000
VERSION     DAZGJW010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.544"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            112..603
                     /locus_tag="ACHKIK_005734"
     CDS             112..603
                     /locus_tag="ACHKIK_005734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO9086618.1"
                     /translation="MSTETIEIFNNSDEWSNQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
BASE COUNT          308 a          126 c          122 g          241 t
ORIGIN      
        1 ccataagcaa tgcaagctta aaactcataa tttaaaaaag ataaatataa aaatatcaat
       61 gagtcaatca ataataattt ggcatcacga gacacatcac agaggaatat tatgagcaca
      121 gaaacaattg aaatattcaa taatagtgat gaatggtcaa atcaactaaa acacgcatta
      181 tcgaaaggag aaaatctggc attactacat ggtttaactc ctgatatcct tgatagaata
      241 tatgcatatg cattcgacta ccatgaaaag ggtaatataa cggacgcaga aatttattat
      301 aaatttttgt gcatttatgc gttcgaaaat catgagtatc taaaagattt tgcatcagta
      361 tgtcagccca aaaagaaata tcaacaagca tatgaccttt acaaactaag ttacaattac
      421 tccccgtatg atgactattc agttatttat cgtatgggtc aatgtcagat tggggctaaa
      481 aatatcgata acgcaatgca atgtttctat cacattatta acaattgtga ggatgatagt
      541 gttaagagta aagcgcaggc atatattgaa ctcttaaacg ataattcaga agataatggc
      601 taagcattac aatcatttgg ttaaatttgc tggttatagt gcagttaacc actataacca
      661 gcacctctta tgttttctta aatcagcatc ttaaaacagt taagagcata ctataaatca
      721 ttatttttca atgcgttaga gaacaatcta ctttatcaaa attgaataag gctatttcag
      781 attacatcac tgaattc
//
LOCUS       DAZGJW010000539          794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZGJW010000539 DAZGJW010000000
VERSION     DAZGJW010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.545"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            35..559
                     /locus_tag="ACHKIK_005735"
     CDS             35..559
                     /locus_tag="ACHKIK_005735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HFO9086619.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            643..>794
                     /locus_tag="ACHKIK_005736"
     CDS             643..>794
                     /locus_tag="ACHKIK_005736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO9086620.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNE"
BASE COUNT          228 a          175 c          178 g          213 t
ORIGIN      
        1 tcaagcatat ttatctatat aaatggagtt ataaatgaaa aaggttctgt ttgccttatc
       61 tgcgcttgcg ctgacctcta cttccgtatt cgcagctgat gcgggcgacg gtactgttaa
      121 attcaccggt gagattgttg acgccgcttg tgttgtgtct ccagacactc aaaaacaaga
      181 agttgttctg ggtcaggtga acaagtctgt atttactgct atgggtgata aatccacagc
      241 aacgccgttc aaaatcaaac tggaaaactg cgatatctcc actttcaaaa atgtggaaat
      301 cagctttaac ggcgttggcg atgcagacaa cagcaaactg gtttctgtaa gcactgaacc
      361 aggtgccgca actggcgtag gtatcggtat ttatgataac accaataccc tggttgacct
      421 gaatactggt aaatctgcta ctgtcctgaa agaaggtcag accgttctgt atttcaccgc
      481 taactatgtc tctaccaaag atgcagtaac cacgggttac ggtaacgctg aagtcgactt
      541 caacctgact tacaactaat cgatcctgac ggaacgatca tctctcaatg gcaacagaga
      601 ctgtgttgcc attattcaca ccaatgactc agggaccgaa tcatgaaacg tattagcttt
      661 gctgtgctgg cacttttaat gaccgcacaa cagtgttttg ctggtggcgt agccttaagc
      721 acgacgcgtg ttatttatga tggcggcaag aaagaagcgt cgctgaccgt tgaaaaccat
      781 aataaaaatg aaga
//
LOCUS       DAZGJW010000540          790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZGJW010000540 DAZGJW010000000
VERSION     DAZGJW010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.546"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>790
                     /locus_tag="ACHKIK_005737"
     CDS             <1..>790
                     /locus_tag="ACHKIK_005737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO9086621.1"
                     /translation="RLRTQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQ
                     LTSATGPDGLEIRREYDELGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTM
                     TWSRYAQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYHAYDSRGQLIAVKDTQG
                     HETRYEYNIAGDLTAVIAPDGSRNGTQYDAWGKAVRTTQGGLTRSMEYDAAGRVIRLT
                     SENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEG"
BASE COUNT          196 a          237 c          231 g          126 t
ORIGIN      
        1 caggctcagg acacagacgg atgccgcagg ccggacaaca gaatacagcc cggatgtggt
       61 gacgggcctc atcacgcgca tcaccacgcc ggatggcagg gcatcggcgt tttactataa
      121 ccaccacagc cagttaacgt cagccaccgg gcctgacggg ctggaaatac gccgggaata
      181 tgatgaattg ggccgtctga ttcaggaaac tgcccctgac ggcgatatca cccgctaccg
      241 ttatgataat ccacacagtg acttaccctg cgcaacggaa gatgccaccg gcagccggaa
      301 aaccatgacg tggagccgtt acgctcagtt gctgagcttc accgactgtt ccggttatgt
      361 aacccgttat gaccatgacc gcttcgggca gatgacggcg gtgcaccgcg aggaaggcct
      421 gagccagtac cacgcatacg acagccgtgg acagttaatt gccgtgaaag acacgcaggg
      481 ccatgaaacg cggtatgaat acaacatcgc cggtgacctg accgccgtca ttgccccgga
      541 cggcagcaga aacgggacac agtacgatgc gtggggaaag gccgtccgta ccacgcaggg
      601 cgggctgacg cgcagtatgg aatacgatgc tgccggacgg gtcatccgcc tgaccagtga
      661 aaacggcagc cacaccacct tccgttacga tgtactcgac cggctgatac aggaaaccgg
      721 ctttgacggc cgcacacagc gttatcacca cgacctgacc ggcaaactta tccgcagcga
      781 ggatgagggg
//
LOCUS       DAZGJW010000541          788 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZGJW010000541 DAZGJW010000000
VERSION     DAZGJW010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.547"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>788
                     /locus_tag="ACHKIK_005738"
     CDS             <1..>788
                     /locus_tag="ACHKIK_005738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO9086622.1"
                     /translation="VSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLP
                     LALISTEGATAWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDP
                     LQGRYITQDPIGLKGGWNFYQYPLNPISNIDPLGLETLKCIKPLHSMGGTGERSGPDI
                     WGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEAAGRCDLVETD
                     NSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDS"
BASE COUNT          219 a          169 c          226 g          174 t
ORIGIN      
        1 gggtgagtga ggaaagccgc cgctggctgg catcgtgcgg cctgaccgtg gagcagatgc
       61 aaaaccagat ggacccggtg tacacgccgg cgcgaaaaat ccacctgtac cactgcgacc
      121 atcgcggcct gccgctggcg ctcatcagca cggaaggggc aacagcgtgg tgcgcagaat
      181 atgatgaatg gggcaacctg ctgaatgaag agaacccgca tcagctgcag cagcttatcc
      241 ggctgccggg gcagcagtat gatgaggagt ccggcctgta ttacaaccgc caccgctatt
      301 atgacccgct gcaggggcgg tatatcactc aggatccgat tgggctgaag gggggatgga
      361 atttttatca gtatccgttg aatccgatct caaatataga tccattagga ttagaaacac
      421 taaaatgcat taagccactg cattcaatgg gcggaactgg tgaaagaagc ggtccagata
      481 tatgggggaa tccgttctat catcaatatc tttgtgtccc agatggtaaa ggggactata
      541 cttgtggtgg ccaagaccaa cggggagaat caaaaggaga tggtctatgg gggccaggta
      601 aagcaagtaa tgatacaaaa gaagctgctg gccgttgtga cctcgttgaa accgataata
      661 gttgtgtgga gaactgttta aaagggaagt ttaaagaggt aaggccgcgt tattctgtat
      721 tgcctgatat attcacacct ataaatttag ggctatttaa aaactgccaa gactggtcta
      781 atgattct
//
LOCUS       DAZGJW010000542          787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZGJW010000542 DAZGJW010000000
VERSION     DAZGJW010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.548"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(55..669)
                     /gene="espF"
                     /locus_tag="ACHKIK_005739"
                     /pseudo
     CDS             complement(55..669)
                     /gene="espF"
                     /locus_tag="ACHKIK_005739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
BASE COUNT          223 a          170 c          192 g          202 t
ORIGIN      
        1 actctctaaa caatcaacag taattaataa ttttaaatct actttttatg cctttttcga
       61 cagttcatag gcagctaaat gatcttttaa tgcctgagca ataggcggta atggccggga
      121 tgcctcggat gcctgtccac ttgtcggtgg cggcggtgcc gggcggcttg gcttaagacc
      181 tgaagtatca agacttttcg atttttcata ggcagccaaa tgatctttta gcgcctgagc
      241 aatgggcggt aaagaccggg atgacccaga taccaacccg cttgtcggcg gcggcggtgc
      301 cgggcggctt ggcttaaggc ttgaagtatc aagacttttc gacttttcat aggcagctaa
      361 atgatctttt aatgcctgag caatgggcgg taaagggcgg gatgccccgg aagcctgtcc
      421 acttgtcggc ggcggtgggg ccggacgaga gggagtaaat gaagtcgccg ggctactcac
      481 attaaaagtc gttctcgcat tgatattcga cgtaccggga gaaaaaggag attgagccct
      541 aatcggattc agacgaacag cctgagggga cactgaaaag ccagataacc ccgcagagct
      601 tacccggctt accgcactag taatatgccg tcctagtgta gaaacagctt gactaattcc
      661 attaagcata attcatgcct ttttatatct aatcaacagg aataattatt aatttgtatt
      721 aaaattaaaa atagttaacg gctataaact attaaatcaa acacacatgg aaacatagaa
      781 actttcg
//
LOCUS       DAZGJW010000543          787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZGJW010000543 DAZGJW010000000
VERSION     DAZGJW010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.549"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>787
                     /locus_tag="ACHKIK_005740"
     CDS             <1..>787
                     /locus_tag="ACHKIK_005740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9086623.1"
                     /translation="FNCFFHPLVGLPPVYAAGLAAMQGHHIQANSTYFFRNGGRPSGV
                     IEVPGSITEENAKKLKGNWDSGYTGENAGKTAILSNGAKYSPTTFSPVDAQTVEQLKM
                     TAEIVCSVFRVPAYKIGVGHPPSSDNVEALEQQYYSQCLQTLIESIELLLDEALETGE
                     NESTEFDVTTLLRMDSERRMKTLGESVKNTLLTPNEARKRENLPPLAGGDALYLQQQN
                     YSLEALSRRDAREDPFASAGKTVSAQLPDGASDGNKAISETEHD"
BASE COUNT          197 a          194 c          239 g          157 t
ORIGIN      
        1 tttaactgtt tttttcatcc gcttgtgggg ctgccgccgg tgtatgccgc aggactggcc
       61 gccatgcagg ggcatcatat tcaggcaaat tcgacgtatt ttttcagaaa tggcgggcga
      121 ccatcaggcg tgattgaggt ccccggcagt attacggaag aaaacgcgaa aaaactgaag
      181 gggaactggg acagcggata tacgggcgaa aatgccggga agacggccat actgagcaac
      241 ggggcaaaat acagccccac gacgttttca ccggtggatg cgcagacggt ggaacaactg
      301 aaaatgacgg ctgaaattgt ctgttcggtg ttccgtgtcc cggcctacaa gattggcgtt
      361 ggccacccgc cttccagtga caacgtggag gcgctggagc agcagtatta ttcccagtgt
      421 ctgcagacgc tgattgagtc cattgagctg ttactggatg aggcgctgga aacgggggaa
      481 aacgagagca cggagtttga cgtcaccacg ctgctgagaa tggacagcga acggcgcatg
      541 aaaacactgg gtgaatcagt gaaaaatacg cttctcacgc ccaatgaggc ccgtaaacgt
      601 gagaacctgc cgccccttgc cggtggtgat gcactgtatc ttcagcagca gaactacagt
      661 ctggaggcgc tgtcccgtcg tgatgcccgt gaggacccgt tcgcgtcggc cgggaaaaca
      721 gtttcagcac aactgcctga cggcgcatct gacggtaata aggcaatcag tgaaacagag
      781 catgatg
//
LOCUS       DAZGJW010000544          785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZGJW010000544 DAZGJW010000000
VERSION     DAZGJW010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.550"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..321
                     /locus_tag="ACHKIK_005741"
     CDS             <1..321
                     /locus_tag="ACHKIK_005741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9086624.1"
                     /translation="REFGECKGKKHYHLMLLVNRDTWCRAGDYRAPGSLAGMIKQAWC
                     SALGVDVGCHATLVHFPAWPAVWLARNDDTGFQQVLERADYLAKEHTKVHCTGERNFG
                     CSRG"
     gene            487..>785
                     /locus_tag="ACHKIK_005742"
     CDS             487..>785
                     /locus_tag="ACHKIK_005742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO9086625.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHC"
BASE COUNT          174 a          215 c          226 g          170 t
ORIGIN      
        1 cgggagtttg gggagtgcaa aggtaaaaaa cactatcacc tgatgctgct ggtcaaccgg
       61 gatacctggt gtcgtgccgg tgattaccgc gctccgggat cactggccgg gatgattaaa
      121 caggcgtggt gcagtgccct gggagtggat gtcgggtgcc atgccacgct ggtgcatttt
      181 ccggcctggc cggcggtgtg gctggcgcgt aatgatgaca ccggctttca gcaagtgctg
      241 gaacgtgctg actatctggc gaaggagcat accaaagttc actgcaccgg tgagcgcaac
      301 tttggctgca gtcgtggctg agccagacag acgagttatc tgacttcagt gtgatgacgt
      361 gttcacggcc accacactga ttttccctta tgagactgaa ctgaaaccgc cattcacacc
      421 ggaccacgct gcccgtaccg ggacggctgt tgcctgcgtg tcgtctgacg gtaaggaaca
      481 tatactatgt acgcaaaatc ctttctcgct cttgatggca acggacgtct gacgggcgcc
      541 cgtacagcac aggccgcacc ttatgctcac tacacctgcc acttgtgtgg cagtgcactc
      601 agataccatc cgcaatacga cactgaactt ccctggtttg aacacactga cgacaggctg
      661 acagagcacg gtcaacagtg cccttatgtc aggccggagc gcagagaaat acagttgatt
      721 aaacgtctgc agcaattcgt accggatgcc ttacccgtgg tgcgtaaagc cagctggcac
      781 tgcag
//
LOCUS       DAZGJW010000545          780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZGJW010000545 DAZGJW010000000
VERSION     DAZGJW010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.551"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..612
                     /locus_tag="ACHKIK_005743"
     CDS             <1..612
                     /locus_tag="ACHKIK_005743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 34 domain-containing
                     protein"
                     /protein_id="HFO9086626.1"
                     /translation="NQLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLRGRYI
                     TQDPIGLKGGWNFYQYPLNPVTNTDPLGLEVFPRPFPLPIPWPKSPAQQQADDNAAKA
                     LTKWWNDTASQRIFDSLILNNPGLALDITMIASRGNVADTGITDRVNDIINDRFWSDG
                     KKTDRCDVLQELIDCGDISAKDAKSTQKAWNCRHSRQSNDKKR"
     gene            633..>780
                     /locus_tag="ACHKIK_005744"
     CDS             633..>780
                     /locus_tag="ACHKIK_005744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HFO9086627.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQL"
BASE COUNT          261 a          153 c          177 g          189 t
ORIGIN      
        1 aaccagctga atgaagagaa cccgcaccag ctgcagcagc tcatccgcct gccggggcag
       61 cagtatgatg aggagtccgg cctgtattat aaccgccacc gctattatga cccgctgcgg
      121 gggaggtata tcactcagga tccgattggg ctgaaggggg gatggaattt ttatcagtat
      181 ccgttgaatc cagttacgaa tacagatcct ctggggttag aagtttttcc tagaccattc
      241 cccttgccaa ttccatggcc caaaagccct gcacagcagc aagcagatga taatgctgca
      301 aaagcattga caaaatggtg gaacgataca gcatcacaaa gaatatttga ctctctaata
      361 ttgaataatc cgggactagc attagatata acaatgatag cttctcgtgg aaatgttgca
      421 gacacaggga taactgatcg tgtcaatgac ataataaatg acagattctg gagtgatggg
      481 aaaaaaaccg acagatgtga cgtacttcag gaactaattg attgtggtga tattagtgct
      541 aaagatgcaa aaagcacaca gaaagcctgg aattgtcgtc actccagaca gtcaaacgat
      601 aaaaaaagat agcccttgtg gaggttcctg caatgtcaaa tacataccag aaaagaaagg
      661 caagtaaaga gtatggttta tataatcaat gtaagaaact aaatgatgat gaattatttc
      721 gcttacttga tgatcacaat tccttgaaaa ggatttcatc tgccagagta ttacagttaa
//
LOCUS       DAZGJW010000546          775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZGJW010000546 DAZGJW010000000
VERSION     DAZGJW010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.552"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>775)
                     /locus_tag="ACHKIK_005745"
     CDS             complement(<1..>775)
                     /locus_tag="ACHKIK_005745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086628.1"
                     /translation="FSQPELDAPISDSFSYLYDNFQIADGDVHIKLLHLNRETKQITP
                     HRIILQKYFIDSFAKTSISDRENNIYPVICNSPDHFTSDIYRHPFRIVLGNKILYPSE
                     ELVKFIRTSKEYLSNMDVINNVIVPQKTIKKNKLSVVSLNSNIKNDVVLSGVMTGTSK
                     VFHLNKSGDLLLTTSKTHGGGIIVIFKDFINNWWKYNLTLITVPIDDKLSDNRINITP
                     MGITIHEIVNGDDKLFFYPTPLKNGGFILHNPLYTNSFPS"
BASE COUNT          257 a          112 c          121 g          285 t
ORIGIN      
        1 gaaggaaatg aattagtgta aagtggatta tgtaagataa aacctccatt ttttaatgga
       61 gtaggataaa aaaacaactt atcatcccca ttgactattt catgtatcgt tatacccatt
      121 ggtgttatat ttatcctgtt atctgacaat ttgtcatcaa taggaactgt tatcaatgta
      181 aggttatatt tccaccagtt atttataaaa tctttaaaaa taactataat cccgccccca
      241 tgagtttttg aagtggttaa caataagtcg cctgatttat ttagatgaaa aacctttgat
      301 gttcctgtca ttactccaga caatacaaca tcatttttga tgttgctatt taaactaacg
      361 acagataatt tgtttttctt tattgttttt tgtggaacaa taacattatt aattacatcc
      421 atattgctta aatattcctt tgaggttctt ataaacttta caagttcttc tgatggatac
      481 aatattttat tcccgagaac tatcctaaat ggatgcctat atatatcact tgtgaagtga
      541 tcaggagaat tacaaataac aggatatata ttgttttctc tgtcagagat acttgtttta
      601 gcaaatgagt ctataaagta tttttgaagg ataatacgat gtggcgttat ttgctttgtt
      661 tctctattga gatgaagaag cttaatatga acatcaccat cagcaatctg aaaattatca
      721 tatagatatg aaaaggagtc tgatattgga gcatctaact cgggttgtga aaaat
//
LOCUS       DAZGJW010000547          775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZGJW010000547 DAZGJW010000000
VERSION     DAZGJW010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.553"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..114)
                     /gene="ariR"
                     /locus_tag="ACHKIK_005746"
                     /gene_synonym="ymgB"
     CDS             complement(<1..114)
                     /gene="ariR"
                     /locus_tag="ACHKIK_005746"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO9086629.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAV"
     gene            complement(143..415)
                     /gene="ymgA"
                     /locus_tag="ACHKIK_005747"
     CDS             complement(143..415)
                     /gene="ymgA"
                     /locus_tag="ACHKIK_005747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO9086630.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(458..694)
                     /gene="ycgZ"
                     /locus_tag="ACHKIK_005748"
     CDS             complement(458..694)
                     /gene="ycgZ"
                     /locus_tag="ACHKIK_005748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO9086631.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
BASE COUNT          221 a          167 c          130 g          257 t
ORIGIN      
        1 gacagcctgc cctaacactg ctgactcttc ttctaacaaa ttacccgaac tgcgaaagta
       61 acttgctaac gctttatcag ttattgcatt atgaattgta gtatcttcaa gcatggtaac
      121 ctctcatctt acttatgaaa ttttaatgta ttctgtttat tttcttacca ttgaacatac
      181 ttttattgtc agctgtcgct gttcttcggt ttggccctcc tgaatcacta ttgctaaaat
      241 cacgaattat gctgtcaatt aaatcgcgtc ggaaagcatc ttcctcagcc gatttcatta
      301 cgagtaattg cttaataaga gtctgcaatg aaaaatttat cttcatgcgc agtatctgga
      361 tcaccgcctg gccagtaatt tcatatttta tacgttcatt atcagatgtc ttcataacga
      421 cttcatagct aacctgttaa ataatcaggc tgttgcatta ttcaaaaagc aacccaatta
      481 gtgcattata atgtttctgt tcctcttccc cagtcgcgtg ttccagtcgg caaagaagtt
      541 tggcacaaag cgactttcga ctcagattac gtccatcttt caaaatctca gtcacaattt
      601 ccccgagagt ttcctgctga gtaggcaagt tagcttttgc aaagtaacga gtgattgctc
      661 ccgcagaatc taaagtcact gaattttgat gcatgctacg cctctgttaa aaatgttaat
      721 caatgttaag tgaggtaaca atagcaaact tgtacgaaat atgtgtacag aatga
//
LOCUS       DAZGJW010000548          774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZGJW010000548 DAZGJW010000000
VERSION     DAZGJW010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.554"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..309)
                     /locus_tag="ACHKIK_005749"
     CDS             complement(<1..309)
                     /locus_tag="ACHKIK_005749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9086632.1"
                     /translation="MATLRELIIKISANSQSFQSELQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     Q"
     gene            complement(360..569)
                     /locus_tag="ACHKIK_005750"
     CDS             complement(360..569)
                     /locus_tag="ACHKIK_005750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO9086633.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVSLEDAMLCW
                     GEDEDKVESDPFAALEDALSFAAMS"
     gene            complement(665..>774)
                     /locus_tag="ACHKIK_005751"
     CDS             complement(665..>774)
                     /locus_tag="ACHKIK_005751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO9086634.1"
                     /translation="PDDREQVLAVYGPVHARLLRQALELIADAESARKK"
BASE COUNT          167 a          256 c          190 g          161 t
ORIGIN      
        1 ctgcgacgcc tgtttcagac gggcattcac ggaactccac tcatcagcca gtgaaatcag
       61 gtgtccggtg gcaaaggcac cggcaaatgc gccagccatt ccgacagcag aaccgcgaat
      121 ttccgtcaac tggctgttca gttctgccag ggcacgtcgc tgctcccggg ctgccgcagc
      181 ggcctgacgc ccgccattct gcagggtccg gtaatattca ctgcccatac gggaagcacg
      241 ctggagctcc gactggaatg actgtgaatt tgccgaaatt ttgataatca gttcacgtaa
      301 cgtcgccatt cacctttctc cgggcaaaaa aacctgccac agcaggtttt catcattatt
      361 tatgacattg ctgcaaagct cagcgcgtct tccagcgccg caaacggatc tgactctact
      421 ttatcctcat cctcgcccca gcagagcatg gcgtcctcca gtgaaacatt catcccctgc
      481 gccccgaaaa ccgctttcac gatctgcgca ttacggatat ccccgcgctc atcgcccagc
      541 ggggacaccc tgtcgaactc catccacatc atcgcctcgc tcgcactcag actgtggccc
      601 agttcggata aggtgcgccc cagacggagc gcaagtcgca tcagaaagcg aatttccggg
      661 cgggctactt ttttctggcc gactctgcat cagcgatcag ttccagtgcc tgacgcagca
      721 accgggcatg taccggacca tagacggcca gcacctgctc acggtcgtcc ggag
//
LOCUS       DAZGJW010000549          773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZGJW010000549 DAZGJW010000000
VERSION     DAZGJW010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.555"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>773
                     /gene="yfdF"
                     /locus_tag="ACHKIK_005752"
     CDS             <1..>773
                     /gene="yfdF"
                     /locus_tag="ACHKIK_005752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFO9086635.1"
                     /translation="GKEGISTCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSN
                     DTDHITIGIDNQEPIELPANYKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDG
                     DPGFIFDKISHKLRHTVTEFDESGNSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLS
                     LGYYDQDDYHIQRGIRINGDSLTQYCSEKARNASAWFESSKAIMAESFATGSDHQVVN
                     ELNGERLREPNEVFKRLGRAIRYNFQVDDAKFRRDNVKE"
BASE COUNT          290 a          133 c          150 g          200 t
ORIGIN      
        1 tggtaaggaa ggaataagca cctgtattaa acatctactt gagcttataa aaaacgccat
       61 acgagtaaac gacgatcctt atagatttaa tattaataat tcctcagtta cttatattga
      121 tattggctcc aatgatacag accatattac tattggtatc gacaaccaag aaccaataga
      181 attacctgcg aactataaag acaaagaact cgtccgtact atcattaatg acaacatagt
      241 tgagaagact catgatatca ataacaagga aatgatcttc agcgcattaa aagaaatata
      301 tgatggagat cctggtttta tcttcgataa gatatcacac aaactcagac atacggtaac
      361 ggagtttgat gagagcggga atagcgaacc aacggactta tttacctggt acggtaaaga
      421 taaaaaaggc gactctctcg ctattgtaat taaaaataaa aacggaaatg attacttatc
      481 tctcggttac tacgatcagg acgactacca cattcaaaga ggaattcgta ttaatggtga
      541 tagtctcacc caatattgta gtgaaaaagc caggaatgct tcagcgtggt ttgaaagcag
      601 taaagctatc atggcagaat catttgcaac aggttccgat catcaggttg taaacgagct
      661 caacggggaa agactgagag aacctaacga agtttttaaa cgtttaggtc gagcaataag
      721 atataatttt caagtggacg atgcaaaatt tagacgcgac aatgtaaaag aaa
//
LOCUS       DAZGJW010000550          769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZGJW010000550 DAZGJW010000000
VERSION     DAZGJW010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.556"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            118..645
                     /gene="sodC"
                     /locus_tag="ACHKIK_005753"
     CDS             118..645
                     /gene="sodC"
                     /locus_tag="ACHKIK_005753"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO9086636.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
BASE COUNT          203 a          197 c          186 g          183 t
ORIGIN      
        1 ggtattatta atcgcagtga taggatattg cacagctatt gcgcgatatc atcagaacgc
       61 tgtttgttac cctgtaacca gcaagctcag tctgttaacg gaattaatga gggttttatg
      121 aaatgtaaaa tcattgctgc cattgccatg ctgacagcag catcatgcgg atacgcagca
      181 gaacaggaag tcccaatgaa ccttgtcagt gctgacggaa aagaagtcag cattggaaaa
      241 ataaccattc aggagacccc ctacggtctg ctgttcacac cagcccttca ctctctgtct
      301 gaaggcattc atggttttca tgtgcacgaa aaaggaaatt gcgccccggc actgaaagac
      361 ggaaaaccgg tcgcagcatt atcggctggc ggtcactttg acccgaaaaa caccggcaaa
      421 catcttggcc cctggtctcc ggatggacac ctgggcgacc tccctgcgct gttcgtgacg
      481 catgacggaa aagcgaacta cccggtcctg gccccgagac tgaactcatt aaaagagatt
      541 aaagggcgtt ctctcatgct tcatgctggc ggtgataacc atcatgacca tccggagccc
      601 ctgggcggtg gtggtgcgag aatggcctgc ggcatcattc aataatcagt caggtaaggg
      661 gcgggcccct tacctttatt cctcaggacg ataaatcctt tctccctgaa aagaacggca
      721 catcctccct ctctgagtta atgtttttgt cgtgacataa gaataattc
//
LOCUS       DAZGJW010000551          752 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZGJW010000551 DAZGJW010000000
VERSION     DAZGJW010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.557"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..663
                     /locus_tag="ACHKIK_005754"
     CDS             <1..663
                     /locus_tag="ACHKIK_005754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO9086637.1"
                     /translation="NLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYI
                     TQDPIGLKGGWNLYTYPLSPVNSMDPLGLYEFKSKNIDDIGIFALAMCNGESINENKE
                     YGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKGNKVT
                     KENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYLGTPNNTYLKYNPKAKGNGVTIIRQG
                     SN"
     gene            665..>752
                     /locus_tag="ACHKIK_005755"
     CDS             665..>752
                     /locus_tag="ACHKIK_005755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFO9086638.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFL"
BASE COUNT          265 a          117 c          169 g          201 t
ORIGIN      
        1 aacctgctga atgaagagaa cccgcatcag ctgcagcagc ttatccgcct gccggggcag
       61 cagtatgatg aggagtccgg cctgtattac aaccgccacc gctattatga cccgctgcag
      121 gggcggtata tcactcagga tccgattggg ctgaaggggg gatggaacct gtatacatat
      181 ccgctgagcc cggtgaatag catggatcca ttaggattat atgaatttaa atcaaaaaat
      241 atagatgata ttggaatatt tgcattggca atgtgtaatg gagaatcaat taacgagaat
      301 aaagaatatg gtggactaat atgtaagaag caaggtgaat atttccccat gaatccgata
      361 agttcaaatg ataatgatag tgtagacttg cgaaatataa aatgccctga aggttcagag
      421 agagtaggcg attatcacac tcacggtttt tactctgacg ataaaggaaa taaagtaaca
      481 aaagaaaatg atgtttatga tagtctaaat ttttcaagca aagatttaac gaattcttat
      541 atgaatggaa tgggaaaaaa agaatacagt agttacttgg gaacaccaaa taacacctat
      601 ctaaaatata atcccaaagc taaagggaat ggagttacaa ttatcaggca agggagtaat
      661 taaaatgaag gcaatacaat gttgttattt ccttgcttta gtctccagtc atttaatggc
      721 tggagttatg ggtgatgata atatattttt aa
//
LOCUS       DAZGJW010000552          752 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZGJW010000552 DAZGJW010000000
VERSION     DAZGJW010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.558"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>752
                     /locus_tag="ACHKIK_005756"
     CDS             <1..>752
                     /locus_tag="ACHKIK_005756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9086639.1"
                     /translation="QLETIKEKLGGVLLTEITTRHIAEFLDLWIEGGKNTMAGSMRSV
                     LSDMFREAIVEGRISQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPTWFPLAMD
                     LALVTGQRREDITNMRFSEIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGTVVEQC
                     RMVSRGDYLISAGIRKNSPDGSLHPDGLTKKFVAARKLTGIQFSENPPTFHEIRSLAG
                     RLYKETCGEEFAQRLLGHTSEKTTKMYLDERE"
BASE COUNT          246 a          157 c          179 g          170 t
ORIGIN      
        1 atcaactgga gacaataaaa gaaaaattag gaggggtttt actgacagaa ataaccactc
       61 gccatattgc cgagtttctt gatttgtgga ttgaaggagg gaaaaacacg atggcaggat
      121 caatgcgttc tgtgttatct gatatgttcc gcgaggccat tgttgaagga cgtatatctc
      181 aaaatccagt aacgccaaca agagcaccga aaatagtagt tacaagagaa cggctgaaac
      241 taaagacata caactgcatc agggaggcag cagatcaact tccgacatgg ttcccattag
      301 ctatggactt agcccttgta acaggacaac gtcgcgaaga cataacaaat atgcggttca
      361 gtgagattta tgatgatcgt ctccacatca ggcaaattaa gacaggaatg atgattgcta
      421 tccccctgtc actcagcctt cctgtcgctg gtttacgact tggtacagta gttgaacagt
      481 gccgcatggt aagtaggggg gattatctaa tcagtgccgg gattagaaaa aacagccctg
      541 acggcagcct tcacccggac ggcctgacaa agaaatttgt cgcagccaga aaattaacag
      601 gtatccagtt cagtgaaaac ccaccaactt ttcacgagat cagaagcctg gctggacgat
      661 tgtacaaaga aacatgtggg gaagaatttg ctcaacgtct acttggccac acatcggaga
      721 agacaacaaa aatgtatctt gatgagagag aa
//
LOCUS       DAZGJW010000553          743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZGJW010000553 DAZGJW010000000
VERSION     DAZGJW010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.559"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..363
                     /locus_tag="ACHKIK_005757"
     CDS             <1..363
                     /locus_tag="ACHKIK_005757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO9086640.1"
                     /translation="KVPAAKITKAYFELGMTFPELYDDSHPEALARNTQKIFRWLDKD
                     TPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYREIVERRDRLVKDVDDFVASAVV
                     LYDQMNRGGPAGNAVVMH"
     gene            386..>743
                     /locus_tag="ACHKIK_005758"
     CDS             386..>743
                     /locus_tag="ACHKIK_005758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9086641.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPL"
BASE COUNT          191 a          162 c          227 g          163 t
ORIGIN      
        1 aaagtaccgg ctgcgaaaat taccaaagcg tatttcgagc tgggaatgac gttcccggaa
       61 ctgtatgacg acagccatcc ggaagccctg gcccgtaata cccagaaaat tttccgttgg
      121 ctggataaag acacccctga tgctgttgaa aaaatgcagg ctctgttacc ggcgatcgaa
      181 aaggcgatgc cgcctttgct ggtggcccgt atgcgcagcc acagttctga atattaccgt
      241 gagatcgtcg aacggaggga tcggctggtg aaggatgtcg atgattttgt tgcgtcagcg
      301 gttgttttgt atgaccagat gaatcgcggc ggcccggcag ggaatgctgt ggtgatgcac
      361 taaaagcacg gtgttcgggg gttttatgag cagcaagctt catggtcttg tctgggaagg
      421 gtgcgccttc accggcatga tcttatccag ggtggcggtt atggcccgtc ttgcagacta
      481 cagcaatgac gagggcgtgt catggcctgc cattgaaact atccggcgtc agatcggtgc
      541 aagaagtgaa tccacagtga aatcggctat tgcagaactg gcgaaagagg gctggctgac
      601 gaaggaagag cgtaaggtcg gtgggcgtaa tgtaagcaat atctatcggc ttaatgtgga
      661 aaaactcgaa gcagctgcgg cggcggcgcg tgagtcatat aaaccgaaaa gaaaaattag
      721 cccggcaaaa aatgacccgt taa
//
LOCUS       DAZGJW010000554          732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZGJW010000554 DAZGJW010000000
VERSION     DAZGJW010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.560"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          238 a          170 c          133 g          191 t
ORIGIN      
        1 catatttctt aaaaatcatt caaaatcaat aaagtagaag aacttcatct ctttagcaaa
       61 cgccattaac aattgccttt ccatttagtt aatcgttttg ttttctttaa gattagtcct
      121 attagacaaa tagcaaaatg gaataactac agcactaaga cgttccgcta tatagctatt
      181 acctgttccc ctgacataag gagcagatat acctcaaccc cctattcccc agtgctgacc
      241 gggagcggcg gcggtataaa aaggaacaag gaacaaatgg cagaacaagc aaccatgttt
      301 gtcccccccg cataaccgta ccattcgcat taagaaatat tcccatatct atcccgcaaa
      361 agaggacccg tcatacatca agtccgtttt actgggcatc agattatcgc cgtaattagc
      421 tcagtgaaag ccgggtaaac ggttagcaag ctcagtggct caaaagtgca tagcgctaag
      481 atgggcctgg ttacacctgc cctttgctgc agatcgtgtt gatcggtgtg atgccgccac
      541 acgtttaagt caatctggaa aactcacaat gcgctcttct ataaagagga ctctttgata
      601 aagtaacccg aaaagggaaa gctaactgat atcaatgtac caccttagta atcatccctt
      661 atatacagac aaaaagaagt tgcctgaaag ccaggtatct aaactaatca ctaatggtat
      721 attttatcag ca
//
LOCUS       DAZGJW010000555          732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZGJW010000555 DAZGJW010000000
VERSION     DAZGJW010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.561"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(68..298)
                     /gene="ynfN"
                     /locus_tag="ACHKIK_005759"
     CDS             complement(68..298)
                     /gene="ynfN"
                     /locus_tag="ACHKIK_005759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HFO9086642.1"
                     /translation="MKKIIVFFNSEPAVVVPAMTGVNTIMREYPNGEKTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAIRLLS"
     gene            complement(303..368)
                     /gene="ynfR"
                     /locus_tag="ACHKIK_005760"
     CDS             complement(303..368)
                     /gene="ynfR"
                     /locus_tag="ACHKIK_005760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YnfR"
                     /protein_id="HFO9086643.1"
                     /translation="MKAPSGAFLLGIYSMDTHILR"
     gene            complement(371..559)
                     /gene="ynfQ"
                     /locus_tag="ACHKIK_005761"
     CDS             complement(371..559)
                     /gene="ynfQ"
                     /locus_tag="ACHKIK_005761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO9086644.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(570..>732)
                     /gene="cspI"
                     /locus_tag="ACHKIK_005762"
     CDS             complement(570..>732)
                     /gene="cspI"
                     /locus_tag="ACHKIK_005762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HFO9086645.1"
                     /translation="FGFITPKDGSKDVFVHFSAIQSNDFKTLTENQEVEFGIENGPKG
                     PAAVHVVAL"
BASE COUNT          233 a          150 c          132 g          217 t
ORIGIN      
        1 gatagtctaa attttacaca aacagacaaa gagaattttc ctgaattatc aatacaatag
       61 catcaaatca actcaagagc cttattgctg cttccagaat ttcttctgaa gtaacatgtc
      121 gatccgcggc tacataaatg actttatgat ctccggtcag agatggaaac cctgcggcca
      181 ttacagtaag gtgtgttttt tcgccatttg gatattcacg catgatggtg ttaactccag
      241 tcatcgctgg cactaccact gctggttcag agttaaaaaa aactatgatt tttttcatga
      301 tgttaccgta gtatgtgagt atccatcgaa tagataccaa gcaaaaaagc tcccgaagga
      361 gccttcattt tcactttttt aaatccaacg acagacggct ggcatttaag tattgtgaaa
      421 tattatcaaa tgtaatcatc attgatttac aaaagataca ttttgccccg aaaggattca
      481 tgtcagaaac atcaaaagat gatgttctat actgggaacc atgacaacac gggcatctaa
      541 agtgaatatg gtttgtaata ttgtctacct caaagcgcca ctacatgaac agcggcagga
      601 cctttaggtc cgttctcaat accaaattca acttcctgat tctcagttaa tgttttgaaa
      661 tcgttgctct gaattgctga gaaatggaca aacacatctt tgctgccatc tttcggcgtg
      721 atgaaaccaa aa
//
LOCUS       DAZGJW010000556          732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZGJW010000556 DAZGJW010000000
VERSION     DAZGJW010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.562"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>732
                     /gene="nleL"
                     /locus_tag="ACHKIK_005763"
     CDS             <1..>732
                     /gene="nleL"
                     /locus_tag="ACHKIK_005763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO9086646.1"
                     /translation="GMVLSGAILGDVKELFSEKSNTINLGGCYIDLSDIQENILSVLD
                     NYTKSNKSILLTMNTSDDKYNHDKVRAAEELIKKISLDELAAFRPYVKMSLADSFSIH
                     PYLNNANIQQWLEPICDDFFDTIMSWFNNSIMMYMENGSLLQAGMYFERHPGAMVSYN
                     SSFIQIVMNGSRRDGMQERFRELYEVYLKNEKVYPVTQQSDFGLCDGSGKPDWDDDSD
                     LAYNWVLLSSQDDGMAMMCSLSHMV"
BASE COUNT          228 a           96 c          164 g          244 t
ORIGIN      
        1 tggaatggtt ttaagtggtg ctatcttagg tgatgtaaag gagctcttta gtgaaaaaag
       61 taataccatt aatctaggag ggtgttacat agatctatct gacatacagg aaaatatatt
      121 atctgtgttg gataactata caaaatcaaa taaatcaatt ttattgacta tgaatacatc
      181 tgatgataag tataaccatg ataaagtaag ggccgctgaa gaacttatca aaaaaatatc
      241 tcttgacgaa ttagcggcgt tccggcccta tgttaagatg tctttggctg attcatttag
      301 tattcatcct tatttgaaca acgcaaatat acagcaatgg ctcgagccta tatgtgatga
      361 cttttttgat actataatgt cttggtttaa taattcaata atgatgtata tggagaatgg
      421 tagtttattg caggcaggga tgtattttga gcgacatcca ggtgcgatgg tatcttataa
      481 tagttccttt atacaaattg taatgaatgg ttcacggcgt gatggaatgc aggaacgatt
      541 tagggaactc tatgaagtat atttaaaaaa tgaaaaagtt tatcctgtca cacagcagag
      601 tgattttgga ttgtgcgatg gctctgggaa gcctgactgg gatgatgatt ccgatttggc
      661 ttataactgg gttttgttat catcacagga tgatggtatg gcaatgatgt gttctttgag
      721 tcatatggtt ga
//
LOCUS       DAZGJW010000557          724 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZGJW010000557 DAZGJW010000000
VERSION     DAZGJW010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.563"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>724
                     /locus_tag="ACHKIK_005764"
     CDS             <1..>724
                     /locus_tag="ACHKIK_005764"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFO9086647.1"
                     /translation="KTLTFRWTKKHFEFCMDNKIFLNHKGKKSYKERNLFLFSKGDKI
                     TIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIASNVKIMGGNHPLN
                     RFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWIGNDVVLKGGIAIG
                     DGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIKWWNYNYSDLPDNN
                     KCDDINYFVEEMNRLISDGNIQ"
BASE COUNT          245 a           99 c          146 g          234 t
ORIGIN      
        1 ataaaaccct taccttcaga tggacgaaaa aacactttga gttttgtatg gataataaaa
       61 tctttttaaa tcataagggg aagaagtcgt ataaagaaag gaatttattt cttttctcaa
      121 aaggtgacaa gataacaatt gaagataatg ttattgctga agaatattca acaatgcctg
      181 tgaaaaattt ctccagtgtc ggcgcttttt catttccgac ctgccatttt tcagggaata
      241 tacgtatagg tcgattttgt agtattgcat caaatgtaaa aataatgggt ggaaatcatc
      301 ctttgaatcg ttttactact cacatgatga cttataacgg tgaatttgat aagtttgcta
      361 tgagtgaatt tgaacgtagc tggacattga aaccattcat taccaaacct gaaaatccga
      421 ttataggaaa tgatgtctgg ataggaaatg atgttgtatt gaaaggtggc atcgcaatag
      481 gtgatggtgc tgttattgct gccaattccg ttgttaccaa agatgtgccc ccatatgcca
      541 tagttgcagg agttcctgca aaaataatca gatttaggtt tgattcaaat gttattgatg
      601 aactgttgag aataaaatgg tggaattata actactcaga tcttccggat aataacaagt
      661 gtgatgatat aaattatttt gttgaagaga tgaatcgatt gatatcagat ggaaatattc
      721 aaga
//
LOCUS       DAZGJW010000558          723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZGJW010000558 DAZGJW010000000
VERSION     DAZGJW010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.564"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            37..687
                     /locus_tag="ACHKIK_005765"
     CDS             37..687
                     /locus_tag="ACHKIK_005765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO9086648.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPIPGGLWDHLGARESHHLADSLARQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
BASE COUNT          238 a          143 c          140 g          202 t
ORIGIN      
        1 atatagttca caaaatgggt tgaaaaggat aataatatgc cagtcatatt aaatttttct
       61 aatggaagtg tattgccgga aaatgagctg gaggctttac gacatattgc aagaagcaat
      121 cagaatgaca ctattactat aggaggccgt aatatgaggc ttcattatat ccagtttatg
      181 gatggtttta gtgtagagcc tattcctggt ggactttggg accatcttgg ggcaagagag
      241 tctcatcatc tcgcagacag cctggcaaga caacttaacg gaggtaacac ttttctgcag
      301 gcatacagct tatatctgga gcagaggcaa gctgccccgc ttgtgcagga aagcgtcata
      361 aaaacactac tagatcgaat aaattcgaat gcatttcccg ttagtttaca agacttttcc
      421 tgtactgagg agcatcttaa ttgtccgata acgctacata ttcctgagac aggtgttttt
      481 gtcagaaatg ctcgaaattc agaaatatgt gcattatatg atcaggaagc attgactgaa
      541 cttatcctgc gtaacgctct ccaccccctc agccgtgacc cctttgctcc agaaatgatt
      601 ataagcaaag acaagtgtca ttttaatata acaaaacaat gtttttacgc attacccata
      661 tacccccttc aacaaaacag tatttaaaaa taaaaacaca atataagaag catacaataa
      721 gtt
//
LOCUS       DAZGJW010000559          720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZGJW010000559 DAZGJW010000000
VERSION     DAZGJW010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.565"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..143
                     /gene="etgA"
                     /locus_tag="ACHKIK_005766"
     CDS             <1..143
                     /gene="etgA"
                     /locus_tag="ACHKIK_005766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HFO9086649.1"
                     /translation="GNTWDAVGAYNAGYFNTPNAVELRRQYAMKIYKTYTKLKNNEQI
                     ID"
     gene            complement(140..>720)
                     /gene="escU"
                     /locus_tag="ACHKIK_005767"
     CDS             complement(140..>720)
                     /gene="escU"
                     /locus_tag="ACHKIK_005767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFO9086650.1"
                     /translation="YFMGHSYANEFANFTRLNAYQTLVVVAFFVFLLWKGVLFGYLLF
                     SVFDFWFQKHEGLKKMKMSKDEVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKK
                     STVIVKNPTHIAICLYYKLGETPLPLVIETGKDAKALQIIKLAELYDIPVIEDITLAR
                     SLYKNIHKGQYITEDFFEPVAQLIRIAIDLDY"
BASE COUNT          236 a          136 c          125 g          223 t
ORIGIN      
        1 gggggaatac atgggatgca gttggtgcgt ataatgcagg ctactttaac acaccaaatg
       61 cagtagaact cagaagacaa tatgcaatga aaatatataa aacatacact aaattaaaaa
      121 acaatgaaca aattatcgat taataatcaa gatctattgc tatacgaatc aattgtgcta
      181 caggttcaaa aaagtcttct gttatgtatt gtcctttatg tatgttctta taaagacttc
      241 ttgccaaagt aatatcttca atcactggaa tatcatagag ttcagccagt ttaatgatct
      301 gtagagcttt ggcatctttt cctgtttcaa taactaaagg caatggagtc tccccgagtt
      361 tatagtatag gcaaatcgca atgtgagtcg ggtttttgac gatcacggtt gattttttga
      421 tgttattagc cagacttcca ctttgtatct cagaatgaag gcgacggcgc tcccctttaa
      481 tttcagggtt accatcagta tccttggctt ctcgtttcac ctcatcttta ctcattttca
      541 ttttcttcag tccctcatgc ttctggaacc agaaatcaaa tactgaaaag agtagatatc
      601 cgaataacac gcctttccat aaaagaaaaa caaaaaaggc aacaacgaca agagtttgat
      661 atgcgttcag tcttgtgaaa tttgcaaact catttgcata tgaatgcccc ataaagtaaa
//
LOCUS       DAZGJW010000560          705 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZGJW010000560 DAZGJW010000000
VERSION     DAZGJW010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.566"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..198
                     /locus_tag="ACHKIK_005768"
     CDS             <1..198
                     /locus_tag="ACHKIK_005768"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086651.1"
                     /translation="FNNHDGNVIIVNTSSRFELRKKYVGTLKINSGVSTVDPHWPPVS
                     KRIKLIFFSPTLHSFSCIGVS"
     gene            234..>705
                     /locus_tag="ACHKIK_005769"
     CDS             234..>705
                     /locus_tag="ACHKIK_005769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086652.1"
                     /translation="MSRRKVGNMGRGDFDRLCNASGLIYNSSSEDDSGGWDAIVEFPL
                     LGGIALLNSLNEQPIQCLVQIKSSDGEKKGVQVKLSNMKRFCDTPLPCFFFFARYNKN
                     ANIESAYLVHINSDIMFQVLKRIRQNDIGKKSPLHKLSFTVNYSDEDEIDLTNEY"
BASE COUNT          211 a          124 c          153 g          217 t
ORIGIN      
        1 tttaataatc atgatggtaa tgtgattatt gtgaatacga gctcaaggtt tgagctccgg
       61 aaaaaatacg ttgggacgtt aaaaatcaac tcgggagtat ccacagttga tcctcattgg
      121 ccccctgtaa gcaagcgcat caagctaata tttttcagcc cgacacttca tagtttttcc
      181 tgcattgggg taagctaaac ctagtatcgt acatatgaaa gagggatgag tttatgagtc
      241 gaagaaaagt tggtaatatg ggacgtggtg attttgacag gctttgcaat gcatctggct
      301 tgatttacaa cagctcctct gaggatgatt ccggagggtg ggatgccatt gtcgaatttc
      361 ctctgttagg tggtatagcc ttactgaata gtttgaatga acagcctata caatgtttag
      421 tgcagataaa gtcttctgac ggagaaaaga aaggtgtaca ggtcaaactt tctaacatga
      481 aacgtttctg tgacacacct ctaccctgtt ttttcttttt tgcgcgttat aataagaatg
      541 ctaatatcga atcagcttac cttgtccaca ttaatagtga tatcatgttt caggttctga
      601 agcggattcg tcaaaatgat ataggaaaaa aatcaccact tcacaaactt tctttcacgg
      661 taaattactc tgatgaggat gagatagacc tgactaatga atatg
//
LOCUS       DAZGJW010000561          704 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZGJW010000561 DAZGJW010000000
VERSION     DAZGJW010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.567"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            13..624
                     /locus_tag="ACHKIK_005770"
     CDS             13..624
                     /locus_tag="ACHKIK_005770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HFO9086653.1"
                     /translation="MFSPMTMVGRSLAQAATQTLRPAVTRAATQAGVGASGMKFVPMQ
                     SNFMINHGKLTNQLLQAVAKQTRSGDTQQWFKQEQITYISRTVNRAIDDYCMSNNSVI
                     GKETKSHIFKAVESAIKQPLDMNCAQTSIGHFLQSNKYFNKKVDEQCGKGVDPITRFN
                     TQTRMIEQVSQEIFEQKFGGLKVSDIKALTQSTIAQHVQDTRL"
BASE COUNT          224 a          134 c          167 g          179 t
ORIGIN      
        1 aaggtttatt gtatgtttag tccaatgaca atggtaggta gatcgttagc tcaggcggct
       61 acacaaactc ttagacctgc ggtcaccaga gctgcgacac aagcgggggt gggagccagt
      121 ggcatgaagt tcgtgcccat gcagtcgaac tttatgatta atcatggcaa actgactaac
      181 cagctacttc aggccgtggc caaacaaacc cgtagcggtg atactcagca atggttcaaa
      241 caggagcaaa ttacttatat atccagaaca gtaaacagag ctattgatga ctattgcatg
      301 agtaataatt cggtaattgg taaggaaacc aaaagccaca ttttcaaggc tgttgaaagc
      361 gcgataaagc agcctctcga tatgaattgt gcacaaacgt cgattggcca tttcttacag
      421 tccaacaaat actttaataa aaaagttgat gagcagtgtg gcaaaggggt ggatccaatt
      481 acacgcttta acacccagac caggatgata gaacaagtct cacaagaaat ctttgaacag
      541 aaatttggtg gtttgaaagt tagtgatatc aaagctctga ctcagagcac gattgcacag
      601 catgtacagg atactcggtt gtaggaataa cctgttatat atgcgtgttg ggcataagtt
      661 aagcttcctg gtgtatagcg tagtctttgc tgatgtaaga aaag
//
LOCUS       DAZGJW010000562          689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZGJW010000562 DAZGJW010000000
VERSION     DAZGJW010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.568"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            235..>689
                     /locus_tag="ACHKIK_005771"
     CDS             235..>689
                     /locus_tag="ACHKIK_005771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9086654.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPASVRLLIQLQRNGNWVTEK"
BASE COUNT          198 a          149 c          207 g          135 t
ORIGIN      
        1 agaataaaaa aatcccgcag agttgcggag ctgcgggaag agatacaaag attaactata
       61 gagaattatt cttatgtcac gacaaaaaaa ttaacgcaga gaaattatta gcgccacagt
      121 cagtttgtga aaatgtgaag atattcagaa tttttattca gtgatgatac aggcatcctc
      181 cgggatgcct gttgtttttg tgcgtgaaca gttatcacag taaagggtga gacaatgggc
      241 aaaggtggcg gcaaggcgca cacgccggta gaggcaaagg acaatcttaa gtccacgcag
      301 atgatgagcg tgattgatgc gattggtgag ggaccgatag aaggtccggt gaagggactg
      361 cagagtattc tggtgaacaa aaccccactg acggacacgg acggcaatcc cgtgatacac
      421 ggtgtgaccg cggtctggcg tgccggggag caggagcaga caccaccgga aggctttgag
      481 tcctccggag ctgaaaccgg actgggcgtg gaagtgacga aggcaaaacc ggtgacgcgc
      541 accattacgt ccgcgaacat tgaccgcctg cgggttacct tcggggtgca gtcactggtg
      601 gagaccacct caaagggtga ccgtaacccg gcatccgtcc gcctgctgat tcagttacag
      661 cgtaacggta actgggtgac agaaaagga
//
LOCUS       DAZGJW010000563          688 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZGJW010000563 DAZGJW010000000
VERSION     DAZGJW010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.569"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..222
                     /locus_tag="ACHKIK_005772"
                     /pseudo
     CDS             1..222
                     /locus_tag="ACHKIK_005772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            261..>688
                     /locus_tag="ACHKIK_005773"
     CDS             261..>688
                     /locus_tag="ACHKIK_005773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO9086655.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNVINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALES"
BASE COUNT          245 a          147 c          113 g          183 t
ORIGIN      
        1 gcacacgctg ccgaagtcta taacaaggat ggtaataaac tcgaccttta tggcaaggtt
       61 accgctctac gttattttac tgatgataag cgtgacgatg gtgataaaac ttatgcccgt
      121 ctcggcttta aaggagaaac gcaaatcaat gatcaaatga ttggttttgg tcactgggaa
      181 tatgatttta aaggctataa cgatgaagcc aacggctcgc gcggcaagaa tctctgattg
      241 agttaagtgc ccatcacaat atgatcactg accttatatt acacaatcat cccaggatga
      301 aaacaatcac tttaaacgac aaccatattg cacatttaaa cgccaaaaac actacaaaac
      361 tggaatattt aaacttaagc aataacaatt tactgccaac caatgacatt gatcaactaa
      421 tatcatcaaa acatctttgg catgtattag ttaacgtcat caacaatgat ccacttgcac
      481 aaatgcagta ctggactgca gtaagaaata taattgatga cactaatgaa gtgaccattg
      541 atttatcagg acttaattta accactcaac caccagggct gcaaaacttc acctctatca
      601 atcttgataa taaccaactc acacattttg atgcaaccaa ctacgataga ctcgtaaagc
      661 taagtctgaa tagtaatgct cttgagtc
//
LOCUS       DAZGJW010000564          683 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZGJW010000564 DAZGJW010000000
VERSION     DAZGJW010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.570"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>683
                     /locus_tag="ACHKIK_005774"
     CDS             <1..>683
                     /locus_tag="ACHKIK_005774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO9086656.1"
                     /translation="QRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTGLKI
                     SGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLKAMA
                     DDGQLTADKVVPALISQLGVLRDEYAAMPETVSGSITKVENAFMAWVGGVNEASGVTK
                     TLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVALAE
                     AQFRGTQIAT"
BASE COUNT          140 a          155 c          239 g          149 t
ORIGIN      
        1 cagcggacag gtaccgcttt ttcggataat gcggccctgt ttgcccgttc ggctgcctcg
       61 atgcgtgaat atggttacag tgctgatgat gtgctgaagg tgacggaggc catttccacg
      121 gggctgaaaa tctccggtgc cagtacggct gaggcgggtt cggtgatcac ccagttcagc
      181 caggcgctgg cacagggtgt gttgcgcggt gaggaattta attcggtcaa tgaaagtggt
      241 gaccggatca ttcgtgcact ggctgcaggc atgggcgtgg cccgtaaaga tctgaaggcg
      301 atggcggacg atggtcaact gacggcggat aaagtcgttc ctgcgttaat cagccagctg
      361 ggggtattgc gtgatgaata tgccgccatg ccagaaacgg tttccggtag tatcacgaag
      421 gtggaaaacg cctttatggc ctgggtgggc ggagtgaatg aggccagcgg ggtgacaaaa
      481 acgctctccg gcgtgctgaa cggtgttgcc ggacagattg ataatgtggc aacagctgtg
      541 ggtgcgctgg ttgccgtcgg ggttgcccgg tactttggca atatggcctc cggagcgatg
      601 tctgccacgg caggacttgt gacggcagca cgtaatgaag tggcactggc ggaagcacag
      661 ttcaggggaa cgcagattgc cac
//
LOCUS       DAZGJW010000565          681 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZGJW010000565 DAZGJW010000000
VERSION     DAZGJW010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.571"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..461)
                     /locus_tag="ACHKIK_005775"
     CDS             complement(<1..461)
                     /locus_tag="ACHKIK_005775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO9086657.1"
                     /translation="MPINLTPYLTASGGLGEIPQDTLSGIRTLAFNGGAQVQMGSTIV
                     TIRSVFLGFFMGSVSPEGLSERALRTALNNVNRLERDLNGGLSGRQILASIPSPYASP
                     PRPSVMQTELVLEQIEKCSFNVNSSSLRATEEALTCPITLCIPEHGVIMRNA"
BASE COUNT          205 a          164 c          118 g          194 t
ORIGIN      
        1 gcatttctca taatcacacc atgctcaggg atgcataatg taatcgggca tgttaatgcc
       61 tcttcagtcg ccctcagaga tgacgagtta acattaaatg agcatttttc tatttgctct
      121 aacaccaact ctgtttgcat tacagaagga cgaggtggag aagcatacgg gctgggtata
      181 cttgctaaaa tctggcggcc ggatagccct ccattaaggt cgcgttcaag acgattaaca
      241 ttattcaatg cagttcgtaa agctctctct gataatcctt cagggcttac acttcccata
      301 aagaagccta aaaagacaga acgtattgta acaatagtgc tccccatctg aacctgagct
      361 cctccgttaa aagccagtgt tcgtatgcca gaaagagtat cctgcggtat ttcccccagt
      421 cccccactcg ctgttagata tggtgttaaa tttattggca taataaaacc cccattaact
      481 tcattaacat tttacttgat ttcctgttga agcatcttac ttatcacaat gctttcacac
      541 accattatca gtcttgcaaa agataagact ccattatcaa gccaaaataa atttaacttc
      601 attaacaaac atcaccatga catgacaaca aaaaaccgga gccagactcc ggttttttga
      661 agctgtcggg ttacttcatc c
//
LOCUS       DAZGJW010000566          681 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZGJW010000566 DAZGJW010000000
VERSION     DAZGJW010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.572"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            40..>681
                     /locus_tag="ACHKIK_005776"
     CDS             40..>681
                     /locus_tag="ACHKIK_005776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO9086658.1"
                     /translation="MKNVKSTENRIAMMLKTKGWSQAELARKLGVSAQSVQYWTTGKT
                     FPRSDKLAQLSVISGYPQSWFLGEDASSTFSSAEKHHTREDSVVFNVLDVEFSCGDGT
                     HVRGDLIDVVRSIELDPEYARRLVGNRAFKNIEIGNARGDSMAPTISPGDLLFLDKTV
                     TYFDGDGIYAFCFDGECYVKRLQKIGSKIMVLSDNPNYQPWSIEKEGMALLYIQ"
BASE COUNT          218 a          139 c          160 g          164 t
ORIGIN      
        1 ttaatattta tgtgtataaa gcttcgacag gaactcacca tgaagaatgt aaaaagcaca
       61 gaaaatcgga tagcaatgat gctgaaaacg aaaggctgga gtcaggctga actagcccgt
      121 aagctgggtg taagcgcgca atcagtacaa tactggacta caggaaagac gtttcccaga
      181 agcgataaac ttgcacagct atcagtaatt agtggttatc cacaatcctg gtttttgggt
      241 gaagatgcct catcgacatt ctcttcagct gaaaaacacc atacaagaga agacagcgtt
      301 gtgttcaatg tactggatgt tgaattcagt tgcggcgacg gaactcatgt acggggagat
      361 ctaattgatg tagtacgctc aatagaactt gatcctgaat atgcccgccg cctggtcggt
      421 aacagagcat tcaagaacat agaaatcggt aacgccagag gggacagcat ggccccaaca
      481 atctccccgg gagacttact gtttttagat aaaacagtaa cttactttga cggcgatggc
      541 atttatgcat tttgctttga tggcgaatgt tacgtcaaac gacttcaaaa aattggaagc
      601 aagatcatgg tcttatcaga caatcccaac tatcagccat ggagcatcga aaaagaggga
      661 atggcgctgc tctatatcca g
//
LOCUS       DAZGJW010000567          680 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZGJW010000567 DAZGJW010000000
VERSION     DAZGJW010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.573"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>680
                     /locus_tag="ACHKIK_005777"
     CDS             <1..>680
                     /locus_tag="ACHKIK_005777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9086659.1"
                     /translation="KGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQSIL
                     VNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGSETALGVEVTKAKPVTRTI
                     TSANIDRLRVTFGVQSLVETTSKGDRNPASVRLLIQLQRNGNWVTEKDVTINGKTTSQ
                     FLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIVGL
                     QVDAEQFGG"
BASE COUNT          163 a          183 c          219 g          115 t
ORIGIN      
        1 aaaggtggcg gcagggcgca cacgcctcga gaggcgaagg ataatctcaa atccacgcag
       61 atgatgagtg tgattgatgc gattggtgag ggaccgatag aaggcccggt gaaggggctg
      121 cagagtatcc tggtgaacaa aaccccgctg acggacacgg acggcaatcc cgtgatacac
      181 ggtgtgaccg cggtctggcg cgccggggag caggagcaga caccgccgga aggttttgag
      241 tcatccggct ctgaaaccgc actgggcgtg gaagtgacga aggcaaagcc ggtgacgcgc
      301 accattacgt ccgcgaacat tgaccgcctg cgggttacct tcggggtgca gtcactggtg
      361 gagaccacct caaagggtga ccgtaacccg gcatccgtcc gcctgctgat tcagttacag
      421 cgtaacggta actgggtgac agaaaaggac gtcaccatta acggcaagac cacctcacag
      481 ttcctggcct cggtgattct ggataatctg cctccccggc cctttaacat ccggatggtc
      541 agggagacgg cggacagcac cacggaccag ctgcagaata agacgctgtg gtcgtcatac
      601 accgaaatca tcgatgtgaa acagtgctac ccgaacacgg ccattgtggg gctgcaggtg
      661 gatgcggagc agttcggcgg
//
LOCUS       DAZGJW010000568          665 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZGJW010000568 DAZGJW010000000
VERSION     DAZGJW010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.574"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..96
                     /locus_tag="ACHKIK_005778"
     CDS             <1..96
                     /locus_tag="ACHKIK_005778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EspF repeat-containing protein"
                     /protein_id="HFO9086660.1"
                     /translation="VQNEQSRPLPDVAQRLMQHLAEHGINTSKRS"
     gene            complement(223..492)
                     /locus_tag="ACHKIK_005779"
     CDS             complement(223..492)
                     /locus_tag="ACHKIK_005779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO9086661.1"
                     /translation="MNILRKLMERLCGCGKHDDCEHGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENVGGMK"
     gene            complement(494..>665)
                     /locus_tag="ACHKIK_005780"
     CDS             complement(494..>665)
                     /locus_tag="ACHKIK_005780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO9086662.1"
                     /translation="PDTDGALITGLTFLDPKDATRVQGFFQHLQVRFGDGPWQDVKGL
                     DEVGSDTGRTGE"
BASE COUNT          157 a          196 c          133 g          179 t
ORIGIN      
        1 gtacaaaatg aacaatcaag acctctgcct gatgtggctc agcgtctgat gcagcatctt
       61 gcagagcatg gcattaatac atctaagcgc tcgtgattat aatataatta cctgtattag
      121 ctctgacctg acagttaccg gttatttata caggtatctg tcagattaca tctaaccaac
      181 aaaaaaccgg agccggactc cggtttttgt gaagctgtcg ggctatttca tcccgccaac
      241 attttcccac gtcccgtcag cacgcaggat ttgcagcggt cttaccacgc actgtatctg
      301 ctttttatcc gcatccagta tcaccacctg cgtgattacc ctgtcctgct ccgggataat
      361 gccattctca tctgactcca ggatgtctgc cggccccagt cgcagctgtg ctgtaagtaa
      421 ctccccgtgt tcacagtcat catgctttcc gcaaccacac agacgctcca taagctttct
      481 caaaatattc atgtcattct cctgttctgc ctgtatcact gcccacttca tccagtccct
      541 taacatcctg ccacggcccg tcgccaaacc tgacctgcaa atgctgaaaa aaaccctgaa
      601 cccgtgtggc atctttgggg tcaagaaagg tcagtccggt gatgagtgcg ccatctgtat
      661 ccggg
//
LOCUS       DAZGJW010000569          664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZGJW010000569 DAZGJW010000000
VERSION     DAZGJW010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.575"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            45..>664
                     /locus_tag="ACHKIK_005781"
     CDS             45..>664
                     /locus_tag="ACHKIK_005781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO9086663.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVP"
BASE COUNT          174 a          162 c          169 g          159 t
ORIGIN      
        1 atcctacttt tttattgcct tcaaataaat ttaaggagtt cgaaatggac cagaagctgt
       61 taacggattt ccgctcagaa ctactcgatt cacgttttgg cgcaaaggcc atttctacta
      121 tcgcggagtc aaaacgattt ccgctgcacg aaatgcgcga tgatgtcgca ttccagatta
      181 tcaatgatga attatatctt gatggcaacg ctcgtcagaa cctggccact ttctgccaga
      241 cctgggacga cgaaaacgtc cataaattga tggatttgtc gatcaataaa aactggatcg
      301 acaaagaaga atatccgcaa tccgcagcca tcgacctgcg ttgcgtaaat atggttgccg
      361 atctgtggca tgcgcctgcg ccgaaaaatg gtcaggccgt tggcaccaac accattggtt
      421 cttccgaggc ctgtatgctc ggcgggatgg cgatgaaatg gcgttggcgc aagcgtatgg
      481 aagctgcagg caaaccaacg gataaaccaa acctggtgtg cggtccggta caaatctgct
      541 ggcataaatt cgcccgctac tgggatgtgg agctgcgtga gatccctatg cgccccggtc
      601 agttgtttat ggacccgaaa cgcatgattg aagcctgtga cgaaaacacc atcggcgtgg
      661 tgcc
//
LOCUS       DAZGJW010000570          657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZGJW010000570 DAZGJW010000000
VERSION     DAZGJW010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.576"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>657
                     /locus_tag="ACHKIK_005782"
     CDS             <1..>657
                     /locus_tag="ACHKIK_005782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HFO9086664.1"
                     /translation="ETASALYQTKYNDNIKLSNSDDFFLRKTQRIITMSNELGNNRLK
                     NSQLEILELKDPVLTEGILYQRRISSLLITTEYHSLALQQVSSFWRVTDPNFGHCDFH
                     SLAQALTFIKNITSNGNFSSLYGSGIVKIHFSESPNNWKYIKLPLIQNESLSRDIYLT
                     TPEKLSATGDSLNIMGHLVPVSFIYDIGGVSNGNRISESTDIKNKIKTLKINGDILQH
                     "
BASE COUNT          242 a          106 c          101 g          208 t
ORIGIN      
        1 ttgaaactgc cagcgcattg tatcaaacaa aatataatga caatattaaa ttatctaata
       61 gcgatgattt ctttttaaga aaaacacaac gtatcattac aatgtccaat gagttaggca
      121 ataatagatt aaaaaactca cagttagaaa tcctagaatt gaaggaccct gtactaactg
      181 aaggtatatt ataccagcgt cgaatatcat ctcttttaat aacaacagaa tatcactcac
      241 tcgcgttaca acaagtatct tctttttgga gagtaaccga tcctaatttt gggcattgtg
      301 acttccattc cttagcacag gctttaacgt ttataaaaaa tataacgagc aatggtaact
      361 tttcatcttt atatggaagt ggtattgtta aaattcactt tagtgagtct cctaacaatt
      421 ggaagtatat taaactccct cttattcaaa atgaatcttt gtcaagggat atatatttga
      481 caactccaga gaaattatcc gcaactggag attcattaaa tattatggga catttagtgc
      541 ctgtcagttt tatttatgac attggaggag tctctaatgg caacagaata agtgaaagca
      601 cagatataaa aaataaaatc aagactctga aaataaatgg tgatatttta caacatt
//
LOCUS       DAZGJW010000571          656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZGJW010000571 DAZGJW010000000
VERSION     DAZGJW010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.577"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..609
                     /gene="espW"
                     /locus_tag="ACHKIK_005783"
     CDS             <1..609
                     /gene="espW"
                     /locus_tag="ACHKIK_005783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFO9086665.1"
                     /translation="ANTNIQQKQIADEVKSLTYLIPSEKKEMWIYKNNGKDNAKPNLG
                     ERDVRMFENISPDDTDKITGRKFSELGEYLYSGNVIKLSQLSIRYLPNINSISLIETK
                     QSLLLHRLYSDEVLQRNGTLIPTPLHEEKSIPADNIKTMLNNIPTYKMLPPFTETQGN
                     CSSGAATFLRKSGAEEKDILACSPRNYGLHHNIKTWDPLVRN"
BASE COUNT          266 a          119 c          106 g          165 t
ORIGIN      
        1 gccaatacca atatccaaca aaaacaaatt gcagatgaag taaaatcact tacatatttg
       61 attccttcag aaaaaaaaga gatgtggata tataaaaaca atggaaaaga taatgcaaaa
      121 ccaaacttag gagagcgaga cgtaagaatg ttcgaaaata ttagtcctga tgatacagat
      181 aagataacag gaaggaaatt ttcagagtta ggtgagtatc tttattcagg aaatgtaata
      241 aaactcagtc agttatcaat tcgttactta cccaatatca actcaatctc attaatagag
      301 acaaaacaga gtttgttgct acatcgatta tattcagatg aagtacttca gagaaatgga
      361 acacttatcc cgacaccact acatgaagaa aaatcaattc cagctgacaa tataaaaaca
      421 atgctcaaca acataccgac ttacaaaatg ttaccgccat tcacagaaac acaaggtaat
      481 tgttcttctg gcgcagccac gtttttacgc aaatcaggcg ccgaagaaaa agatattctt
      541 gcatgtagcc cccgaaatta tgggctgcat cataacataa aaacatggga ccccttggtt
      601 agaaattaag gatccaaata ttatatattt catataaagc aaggaaaata ttgcct
//
LOCUS       DAZGJW010000572          655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZGJW010000572 DAZGJW010000000
VERSION     DAZGJW010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.578"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>655
                     /locus_tag="ACHKIK_005784"
     CDS             <1..>655
                     /locus_tag="ACHKIK_005784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin B"
                     /protein_id="HFO9086666.1"
                     /translation="TKRNALYHQLDSDIKERIAFLDISHYAYPGSLLEKLQLSGYVFS
                     DINIITEYLLSSYGISGHYSHGVVYPAPSDKLFELLRRHTNSNSDWIEKIIPYVYDIL
                     SGNVSSFLHSSLSEEQKKILSDIKLEISESVSEQYFMKLTEQKSSVIGIKYSVDFDRY
                     NENLFLSLPINQNLTLPFMYRYFEMLYDIHIGILENRANRDFIYRKFSSLNLDFLIN"
BASE COUNT          233 a          104 c           97 g          221 t
ORIGIN      
        1 aaacaaaacg aaacgcactt taccatcaat tagatagtga tatcaaagag cgaatagcct
       61 ttctcgacat atcccactac gcatatccag gtagtttatt agaaaaatta cagttaagtg
      121 gctacgtttt ttctgacatc aacataatta cagaatactt attgtctagt tacggaattt
      181 ctgggcatta ttctcacggc gtggtatacc cagctccatc tgataaatta tttgagctat
      241 taagaagaca tacaaattcc aattctgatt ggatagaaaa aataatccca tatgtttatg
      301 atatcttatc aggtaatgtg tccagcttct tgcactcttc cttatcagaa gaacaaaaaa
      361 aaatattgag tgatataaaa ttagaaatta gtgaatctgt atcagaacag tatttcatga
      421 agttgacaga acaaaaatca tcagttattg gcattaaata ctctgttgat tttgatagat
      481 acaatgagaa tttatttttg tctttaccta taaatcaaaa tttaacccta cctttcatgt
      541 atcgctactt cgagatgtta tatgacattc acattggaat cttagaaaac agggcaaata
      601 gagattttat atataggaag ttttcatctt taaatttgga ttttctaatt aatga
//
LOCUS       DAZGJW010000573          653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZGJW010000573 DAZGJW010000000
VERSION     DAZGJW010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.579"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..392
                     /locus_tag="ACHKIK_005785"
     CDS             <1..392
                     /locus_tag="ACHKIK_005785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO9086667.1"
                     /translation="FVTAKEAAKHNRQDVLCCVSCGCPLTLQRGNDGQPPWFEHDQMT
                     VAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVMCDEDYEGEKCCPRCG
                     TGVYSRAWGRQEVPSEDARADNPLQRL"
BASE COUNT          137 a          143 c          209 g          164 t
ORIGIN      
        1 attttgttac ggcgaaagaa gctgcgaagc acaaccgaca ggacgttttg tgctgtgtgt
       61 cctgtggatg cccgttaaca cttcagcggg gcaatgacgg acaaccaccg tggtttgaac
      121 atgaccagat gactgtcgct gaaaaaatcc tgctgcgatg tacctggctt gacccggcgg
      181 agaaagaggc ccgtcgtttg catctgcagg gcatgacggt tccggattat acggtgaagg
      241 tgagaaagtg gttttgtgtg atgtgtgacg aagattatga gggggaaaag tgctgcccac
      301 gctgcggtac cggggtatac agcagggcgt gggggcggca ggaggtgccg tcggaagatg
      361 ccagggctga taatccgtta cagaggctgt aatggttgcc tccggagcag tttgcggtga
      421 tgcctttccc gggaatggat tgccagtgcg gggactgtgg agcttaattc cggtgttggt
      481 ggcctttccg ttgattttat gccaacagcc ccctgtgact gacaggctgc ctcgtcattc
      541 cattcgtatc tccagtaaca gggggttgta ttgtatttta ttgtgccggt tcaggtgtgc
      601 gtttcccggc gcgtctgcac cggcttaacc aaattcaaca gggataaaat agt
//
LOCUS       DAZGJW010000574          650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZGJW010000574 DAZGJW010000000
VERSION     DAZGJW010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.580"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..642
                     /locus_tag="ACHKIK_005786"
     CDS             <1..642
                     /locus_tag="ACHKIK_005786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
                     /protein_id="HFO9086668.1"
                     /translation="HLAGFSGVLQADAYAGFNELYRDGRITEAACWAHARRKIHDVHV
                     RTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLESWLREKMKTL
                     SRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKNYLFFGSDHG
                     GERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVALPTE"
BASE COUNT          155 a          177 c          204 g          114 t
ORIGIN      
        1 catcttgcgg ggttcagtgg tgtactgcag gcggatgcat acgccgggtt caacgagctg
       61 taccgggatg gccggataac ggaagccgcc tgttgggctc acgcccgccg taaaatccac
      121 gatgtgcacg ttcgcacccc gtcagccctg acggaggaag cgctgaaacg gatcggcgaa
      181 ctgtacgcca tcgaggcaga gataagggga atgacggcgg agcagcgcct tgccgaacgt
      241 cagttgaaaa cgaaaccgct gctgaaatcc ctggaaagct ggctgcgtga aaagatgaaa
      301 accctgtcgc gacactcaga actggcgaaa gcgttcgcat acgccctgaa ccagtggccg
      361 gcgctgacgt actatgcaga tgatggctgg gctgaggcgg acaataacat cgctgaaaat
      421 gcgttgcgga tggtcagtct gggccgcaaa aactacctgt tcttcggttc ggatcatgga
      481 ggagagcggg gagcgctgct gtacagcctg atcgggacgt gcaaactgaa cggagtggag
      541 ccagaaagct acctccgcta tgtccttgac gtcatagccg actggccgat aaaccgggtc
      601 ggcgaactgc tcccctggcg cgtagcactg ccgactgaat aacacatccc
//
LOCUS       DAZGJW010000575          643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZGJW010000575 DAZGJW010000000
VERSION     DAZGJW010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.581"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(99..641)
                     /locus_tag="ACHKIK_005787"
     CDS             complement(99..641)
                     /locus_tag="ACHKIK_005787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9086669.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKEIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT          197 a          140 c          112 g          194 t
ORIGIN      
        1 agataataat cctttcatct taatgcctct taaattaaaa agccgccatg gggatagcgg
       61 ccatgtttat tacctggaga aacttacaat tttatttatt aatcctgcgc cacacgcata
      121 accagagaag ataacacatc acctgattgc actgagttat ctgtcagttt caggtatttt
      181 gctttaaacg gaatagcata ttgaccttcc gctgttttat aactctcaat gttaaaaggt
      241 gcagaaacat ccaggacatc gctgttatca cctaaataga gatagtaacc cacggtatcg
      301 ttgctactgg ctatctcctt atcgttaccg gttattttat cgttggtaat caacgagagt
      361 tttacaccag caacttcttt cgggcagcta atttttaaca tgctgctggt tacaccgact
      421 gcatcatcat aggtagaata tttagcttta ccttcaaaga cagtggttgg tacagtcggc
      481 atattaatac ttactgaatc accacccgct tcgaatttac acggagattc tgcaatattc
      541 cccttaatag ttaatgttcc ggtatcaacg tctgcagcaa atactccagc gctcataaaa
      601 aaagaagaaa agacagcggc agcaataatt gaacgtttca taa
//
LOCUS       DAZGJW010000576          643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZGJW010000576 DAZGJW010000000
VERSION     DAZGJW010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.582"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..251)
                     /locus_tag="ACHKIK_005788"
     CDS             complement(<1..251)
                     /locus_tag="ACHKIK_005788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO9086670.1"
                     /translation="MRIYRRKCKCCNEWFIPKYQNQYWCNEICGTKIALERRSKEREK
                     AEKAAEKKLRREEQKQKDKLKIRKLALKPRSYWIKQAQQA"
     gene            complement(226..>643)
                     /locus_tag="ACHKIK_005789"
     CDS             complement(226..>643)
                     /locus_tag="ACHKIK_005789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO9086671.1"
                     /translation="GTFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIR
                     TDNRPENLRECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCF
                     DDLELAVLVAEEARDKYYGDFSGNERSTYANLSKEM"
BASE COUNT          146 a          149 c          107 g          241 t
ORIGIN      
        1 gcttgttggg cttgtttaat ccagtaactg cggggcttta aggcgagttt tcgaatcttc
       61 agtttatctt tctgtttctg ctcctctcgt cgtagtttct tctctgctgc tttttccgct
      121 ttttcgcgtt ctttacttcg tcgttcgagt gctatcttgg ttccacaaat ctcattacac
      181 caatattgat tttgatattt tggtataaac cattcattgc aacatttaca tttccttcga
      241 tagattcgca taagtgctcc tttcgttgcc ggaaaaatca ccgtaatact tatctcgggc
      301 ttcttcagca actagtaccg ctaactccag atcatcaaag catccgaagt gtttactctt
      361 gccatggaat cctagcctaa cattccattt tttctgtcgt ttgtgccaag taacccctct
      421 gcaacctgat ttgctattct ttcggatcct tatatttctt gaattttcta ttggcaggca
      481 ttctcttaaa ttttctggcc tattgtcggt cctaattcca ttaacgtggt caatttgacc
      541 agcaggccaa cgattatgag ttatgtaaaa aactaagacg tgagttttat atctacgccc
      601 atctatcatg atcattgaat aaccgttgga atcaaaagtt cca
//
LOCUS       DAZGJW010000577          640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZGJW010000577 DAZGJW010000000
VERSION     DAZGJW010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.583"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(60..635)
                     /locus_tag="ACHKIK_005790"
     CDS             complement(60..635)
                     /locus_tag="ACHKIK_005790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO9086672.1"
                     /translation="MPLTSDIGSRSFNLGLEVFRARIAANGRGDITVGGETVSIVYDA
                     TNGRFSSSGGNDGLLSELLILGFNNGPRALSERMLSMLSDSGEAQSQESIQDKISQCK
                     FPVSSGNFQCPPESIQCPITLERPEEGVFVKNSDSSAVCTLFDVDALSRVVNDGSVHP
                     LTRAPITPSMIVKPEECKYDPARGSFIIKDS"
BASE COUNT          194 a          143 c          116 g          187 t
ORIGIN      
        1 atgcctgtgg tgtttttata taaattatcg gaagttaata ttgtttcatt tgaaacatct
       61 taactatctt ttataataaa gcttcccctt gcagggtcat atttacactc ttctggttta
      121 acaatcattg atggggttat tggagctcgt gtcagagggt gaactgaacc gtcattaacc
      181 acgcgagaaa gtgcatcaac atcaaataaa gtgcatactg ctgaactatc tgaatttttg
      241 acaaacactc cttcttcggg tctctctagt gtaattggac actgaataga ctctggcggg
      301 cactggaaat ttcctgaact aacaggaaac ttacattgag atattttgtc ctgaatactc
      361 tcttgcgatt gtgcttcacc tgagtccgaa agcatactta gcattctctc actaagggct
      421 cgaggaccat tattaaatcc caaaatcaat aactcagaaa gcaatccgtc attaccgcca
      481 ctggatgaaa agcgcccatt agtagcatca tacacaatac tgacagtttc accacctact
      541 gtaatatctc cgcgcccatt ggctgcaatt cgggcacgaa aaacctccag cccaagatta
      601 aatgaacgtg atccaatatc tgaggttaat ggcataacaa
//
LOCUS       DAZGJW010000578          639 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZGJW010000578 DAZGJW010000000
VERSION     DAZGJW010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.584"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>639)
                     /locus_tag="ACHKIK_005791"
     CDS             complement(<1..>639)
                     /locus_tag="ACHKIK_005791"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO9086673.1"
                     /translation="FKYVRDFFVSPGEFRQTGTDASRTINEKQKPVIMFLVVGETARS
                     QNYALNGYSRGTNDFTKKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSRKAVN
                     SENVLDILYRTGVNLLWIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDEVMLK
                     NIDEYINNNSEDKLIVFHLMGSHGPTYYLRYPEPHKYFKPTCDRSDIENCTHE"
BASE COUNT          193 a          129 c          102 g          215 t
ORIGIN      
        1 ctcatgcgta caattttcga tatcactacg atcacatgtt ggtttaaaat atttatgtgg
       61 ctcaggataa cggagatagt aggtaggacc atgactaccc atcaaatgaa atacgataag
      121 tttatcctct gagttattat ttatatactc atcaatattt tttagcataa cctcatcata
      181 acatgagttt ttcttgcata atgtattatc tgaatttgat ggctcaatat taattgtagg
      241 gattcgctta catacacctt tacatccccc gtcattttca atccagagta aatttacccc
      301 cgttctgtac agaatatcca gtacattttc cgaatttacc gctttgcggg aattgaactc
      361 ttttcgcttc atatctgaaa acatgcatgg aacagatatg gctgtggatg tgccacacga
      421 ctgcacatta tgaaaggata taagttcatt atatttcttt gtaaaatcat tggtaccacg
      481 agaatatcca ttcagagcat agttttgact tcttgctgtc tccccgacaa ctaaaaacat
      541 aattaccggc ttttgttttt catttattgt gcgggatgca tccgttccag tctgacgaaa
      601 ctcgccagga gaaacaaaaa aatcccttac atatttaaa
//
LOCUS       DAZGJW010000579          629 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZGJW010000579 DAZGJW010000000
VERSION     DAZGJW010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.585"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>629)
                     /locus_tag="ACHKIK_005792"
     CDS             complement(<1..>629)
                     /locus_tag="ACHKIK_005792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO9086674.1"
                     /translation="DNNHVLKLSSPITVTVECKGGYYFINSSGDKSDITYKVNGLSII
                     PENFFTLLSGNFKADWRWDVSKETFPKDKFAGYVKSVFSKIDFYKQCGVINPQNANTA
                     YFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLNHENMASSAYKHKNNKVLYELL
                     NTRDMILNELHKHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLN"
BASE COUNT          213 a          125 c           97 g          194 t
ORIGIN      
        1 aatttagcaa agtaaggata tatttatcac caaaaatagt actaaatcta tcgcctgtat
       61 gatccccaag gaagatacaa ggggttatcg catcgtcctt taaaaaaaca tgtttgtgca
      121 actcattcaa tatcatgtct ctcgtattta ataactcgta gagcacttta ttatttttat
      181 gcttgtatgc agatgaagcc atgttctcgt gatttaataa ttcacataat aaactccaac
      241 ccttttccct gatgcctata tgccctgaaa cgagcaatgc ataaagtaca gcgcctactc
      301 ttccatctgt atcgccaaaa taagccgtgt tggcattttg tgggttaatc acaccacatt
      361 gtttataaaa gtctatttta gaaaacacag atttaacata gccagcaaac ttatccttag
      421 gaaacgtctc tttagagaca tcccaacgcc agtcagcttt aaaattaccg ctaagaagag
      481 taaagaaatt ttcaggtatg atagaaagcc cattaacttt ataagttatg tctgatttat
      541 ctccagagga attaataaaa taataaccgc ccttacactc aacagtaacg gtaatgggag
      601 acgataattt taacacatgg ttattgtct
//
LOCUS       DAZGJW010000580          628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZGJW010000580 DAZGJW010000000
VERSION     DAZGJW010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.586"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(14..171)
                     /locus_tag="ACHKIK_005793"
                     /pseudo
     CDS             complement(14..171)
                     /locus_tag="ACHKIK_005793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701877.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            500..>628
                     /locus_tag="ACHKIK_005794"
     CDS             500..>628
                     /locus_tag="ACHKIK_005794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086675.1"
                     /translation="MPIFLNFTAGSILPENELASLRYIVQQNQNDTVIIKERYKMDI"
BASE COUNT          197 a          142 c           86 g          203 t
ORIGIN      
        1 gccgcggatc ctattattct agtatgaaat ccctaaccga tttcaaattc tccagactgc
       61 tcagattctc ttttatccca cgctggcgct gataaatctc gtcattgcaa tcgacctgcg
      121 ctatcgctgc cgcagttctt ccagtttctg catcgataac tccatcttct gagcaataaa
      181 cctcacctat gcaatatttt atgtcaatta tttcaattgc attaatatct tgaatggatt
      241 acatagagtt aatgtatcca gtactcccta ttctcttgca tataatacat gttgcaactt
      301 acatctcagc gctatgaaaa aacaccacct ctctctttat gaaattctgg atttaccaag
      361 cgctaattta tcatttcaat ctacctttaa gcattgcatt tatctcccta cgaggtcata
      421 ctttaggaag ttaaatatga atgataatat acctacagcg cgaaatcaca aacaatcgac
      481 ttgtattaca gaaaaaaaca tgcctatctt tttaaacttc acagcaggca gtatcttacc
      541 tgagaatgag ctagcatctt tacgttatat tgtgcagcaa aatcaaaatg atactgtaat
      601 cataaaagaa cgttataaaa tggatatc
//
LOCUS       DAZGJW010000581          625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZGJW010000581 DAZGJW010000000
VERSION     DAZGJW010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.587"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..>625
                     /locus_tag="ACHKIK_005795"
     CDS             2..>625
                     /locus_tag="ACHKIK_005795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO9086676.1"
                     /translation="MKEEGAVAVATAIEAGDERRARAKFHWQFLEHYPAAQDCAYKFL
                     VCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPINVTFDKLAPEVQNAV
                     MVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEVNAMYPELKLHAIGWV
                     KHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARARANQQ"
BASE COUNT          174 a          147 c          165 g          139 t
ORIGIN      
        1 aataaaggaa gaaggtgcag tcgctgtagc cactgccatt gaagccggtg atgaacgtcg
       61 cgcccgcgca aaatttcact ggcaattcct ggaacattat ccggctgctc aggactgcgc
      121 ttataaattt cttgtttgcg aggataaacc cggtattccc cgccctgccc tcgattcctg
      181 ggatgctgaa tatatgcagg aaaaccgctg ggatgaggag tctgcttcct ttgtcccggt
      241 tgagactgaa tcagatccga taaacgtcac ttttgacaag ctggcccctg aagtacagaa
      301 cgctgtcatg gttaagttcg acacatgtga aaacatcacc gttgatatgg tgattagcgc
      361 gcaggaactg ttgcaggaag acatggcaac attcgacgga catatcgttg aagcgttgat
      421 gaaaatgcca gaagttaacg ccatgtatcc ggagcttaag ctgcatgcca tcgggtgggt
      481 taagcataaa tgtaagccag gtgccaaatg gcccgaaatt caggcagaga tgcgcatctg
      541 gaaaaaacgt cgcgaaggtg aacgcaagga aaccggaaaa tacacgtctg ttgttgatct
      601 cgcccgcgcc agagccaatc aacag
//
LOCUS       DAZGJW010000582          620 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZGJW010000582 DAZGJW010000000
VERSION     DAZGJW010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.588"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>620
                     /locus_tag="ACHKIK_005796"
     CDS             <1..>620
                     /locus_tag="ACHKIK_005796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HFO9086677.1"
                     /translation="KLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYK
                     RLTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEI
                     RTPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCW
                     LAGVHAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITRE"
BASE COUNT          198 a          146 c          133 g          143 t
ORIGIN      
        1 aagctgggtt ggtactggtt aagtcgagta agaggaaaag tacaatatgc agacctagga
       61 gcggaaaact ggaaacctat cagcaactta catgatatgt catctagtca ctcaaagact
      121 ttaggctata agaggctgac taaaagcaat ccaatctcat gccaaattct attgtataaa
      181 tctcgctcta aaggccgaaa aaatcagcgc tcgacacgga ctcattgtca ccacccgtca
      241 cctaaaatct actcagcgtc ggcaaaggag ccatgggttc tagcaactaa cttacctgtt
      301 gaaattcgaa cacccaaaca acttgttaat atctattcga agcgaatgca gattgaagaa
      361 accttccgag acttgaaaag tcctgcctac ggactaggcc tacgccatag ccgaacgagc
      421 agctcagagc gttttgatat catgctgcta atcgccctga tgcttcaact aacatgttgg
      481 cttgcgggcg ttcatgctca gaaacaaggt tgggacaagc acttccaggc taacacagtc
      541 agaaatcgaa acgtactctc aacagttcgc ttaggcatgg aagttttgcg gcattctggc
      601 tacacaataa caagggaaga
//
LOCUS       DAZGJW010000583          619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZGJW010000583 DAZGJW010000000
VERSION     DAZGJW010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.589"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..383
                     /locus_tag="ACHKIK_005797"
     CDS             <1..383
                     /locus_tag="ACHKIK_005797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO9086678.1"
                     /translation="LVEDHRGKTVYDVASGDSLFISELGPLPENVTWLSPYGEYQKWN
                     GTSWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLDIATEEEASLLAAWK
                     TYRVLLNRVNTAVAADVEWPVAPQ"
     gene            complement(427..>619)
                     /locus_tag="ACHKIK_005798"
     CDS             complement(427..>619)
                     /locus_tag="ACHKIK_005798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFO9086679.1"
                     /translation="DGDMPGSRVKIGSNVDIGANCCIIGLSRKIGDNVKIGAMSFINK
                     DIPSNCTYITKKSGVVLYK"
BASE COUNT          186 a          120 c          143 g          170 t
ORIGIN      
        1 atctcgttga agaccatcgg ggtaaaacgg tttatgacgt ggcatcaggg gactcgttat
       61 ttatttctga actcggtcca ttaccggaaa atgttacctg gttgtcgccg tatggagagt
      121 atcagaagtg gaacggcaca tcctgggtga aagatgcaga agcagaaaaa ctgtttcgga
      181 taagggaggc ggaagaaaca aaaaacagcc tgatgcaggt agccagcgag catattgcgc
      241 cacttcagga tgccgtagat ttggatattg caacggagga agaggcatcg ttactggctg
      301 catggaagac ataccgggta ttgttgaatc gtgttaatac agcggtagca gcggatgttg
      361 agtggccagt cgccccacaa taaagagaaa aagccatcga ttgaaatata gatggcttta
      421 tgtactctat ttatacaata caacaccgct ctttttagtt atatatgtgc agttcgatgg
      481 tatatcttta tttataaaag acattgcacc tatttttaca ttatccccaa ttttacgtga
      541 taatccaatg atgcaacaat tagctccgat atcaacgtta ctaccaattt ttactcttga
      601 accaggcatg tcaccatct
//
LOCUS       DAZGJW010000584          619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZGJW010000584 DAZGJW010000000
VERSION     DAZGJW010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.590"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>619
                     /locus_tag="ACHKIK_005799"
     rRNA            <1..>619
                     /locus_tag="ACHKIK_005799"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          142 a          138 c          201 g          138 t
ORIGIN      
        1 atggagccga ccttgaaata ccacccttta atgtttgatg ttctaacgtt gacccgtaat
       61 ccgggttgcg gacagtgtct ggtgggtagt ttgactgggg cggtctcctc ctaaagagta
      121 acggaggagc acgaaggttg gctaatcctg gtcggacatc aggaggttag tgcaatggca
      181 taagccagct tgactgcgag cgtgacggcg cgagcaggtg cgaaagcagg tcatagtgat
      241 ccggtggttc tgaatggaag ggccatcgct caacggataa aaggtactcc ggggataaca
      301 ggctgatacc gcccaagagt tcatatcgac ggcggtgttt ggcacctcga tgtcggctca
      361 tcacatcctg gggctgaagt aggtcccaag ggtatggctg ttcgccattt aaagtggtac
      421 gcgagctggg tttagaacgt cgtgagacag ttcggtccct atctgccgtg ggcgctggag
      481 aactgagggg ggctgctcct agtacgagag gaccggagtg gacgcatcac tggtgttcgg
      541 gttgtcatgc caatggcact gcccggtagc taaatgcgga agagataagt gctgaaagca
      601 tctaagcacg aaacttgcc
//
LOCUS       DAZGJW010000585          616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZGJW010000585 DAZGJW010000000
VERSION     DAZGJW010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.591"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..67
                     /locus_tag="ACHKIK_005800"
     CDS             <1..67
                     /locus_tag="ACHKIK_005800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein O"
                     /protein_id="HFO9086680.1"
                     /translation="VTASKPKLDLTNTDWIYGVDL"
     gene            64..>616
                     /locus_tag="ACHKIK_005801"
     CDS             64..>616
                     /locus_tag="ACHKIK_005801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO9086681.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELVH"
BASE COUNT          163 a          162 c          171 g          120 t
ORIGIN      
        1 cgtgacagcc agcaaaccaa aactcgacct gacaaacaca gactggattt acggggtgga
       61 tctatgaaaa acatcgccgc acagatggtt aactttgacc gtgagcagat gcgtcggatc
      121 gccaacaaca tgccggaaca gtacgacgaa aagcctcagg tacagcaggt agcgcagatc
      181 atcaacggtg tgttcagcca gttactggca actttcccgg cgagcctggc taaccgtgac
      241 cagaacgaac tgaacgaaat ccgccgccag tgggttctgg ctttccggga aaacgggatc
      301 accacaatgg aacaggttaa cgcaggaatg cgcgtagccc gtcggcagaa tcgaccattt
      361 ctgccatcac ccgggcagtt tgttgcatgg tgccgggaag aagcatccgt tatcgccgga
      421 ctgccaaacg tcagcgagct ggttgatatg gtttacgagt attgccggaa gcgaggcctg
      481 tatccggatg cggagtctta tccgtggaaa tcaaacgcgc actactggct ggttaccaac
      541 ctgtatcaga acatgcgggc caatgcgctt actgatgcgg aattacgccg taaggccgca
      601 gatgagcttg tccata
//
LOCUS       DAZGJW010000586          614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZGJW010000586 DAZGJW010000000
VERSION     DAZGJW010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.592"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..588
                     /locus_tag="ACHKIK_005802"
     CDS             <1..588
                     /locus_tag="ACHKIK_005802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO9086682.1"
                     /translation="EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASSGNPDADA
                     EAAFGQFVAANLQPTGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIV
                     SQYVGDQLVLVNAPDIYLADDGGVAVDMSREASLEMQSEPTGDSTTASPVELVSMFQT
                     GSVAIRAERWINWRRRRTAAVAVITGVNYGSASGG"
BASE COUNT          115 a          161 c          220 g          118 t
ORIGIN      
        1 gaagcggtgg tggcgcgtct ggatacagac tttgtggacc cgaaaaaagc ggcggtggca
       61 gatgtctccc cggcgtccat cacccatgat gtgaagggca cggcatcaag cggtaacccg
      121 gatgcggatg cagaggcggc gtttggacag tttgtggcag caaacctgca gcccaccggt
      181 gcggtctggc tgatgtccag caccaatgct ctggcgctgt ccatgcgtaa aaatgcgctg
      241 ggtcagaaag aatacccgga catgaccctg ctgggtggct ccttccaggg gctgccggtg
      301 attgtctccc agtacgtggg tgatcagctg gtgctggtga atgccccgga tatttatctg
      361 gcggatgacg gcggcgtggc agtggatatg tcccgcgagg catcactgga aatgcagtct
      421 gagccgaccg gcgacagtac cacggcgtcg ccggtggagc tggtttccat gttccagaca
      481 ggcagcgtgg ccatccgtgc ggagcgctgg atcaactggc gtcgtcgccg taccgcggcg
      541 gtggcggtga tcaccggagt gaactatggc agtgcgtccg gcggctgagt ctgataagga
      601 ggacgggagg cgtg
//
LOCUS       DAZGJW010000587          611 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZGJW010000587 DAZGJW010000000
VERSION     DAZGJW010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.593"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..473)
                     /locus_tag="ACHKIK_005803"
     CDS             complement(<1..473)
                     /locus_tag="ACHKIK_005803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO9086683.1"
                     /translation="MPVDLTPYILPGVSFLSDIPQETLSEIRNQTIRGEAQIRLGELM
                     VSIRPMQVNGYFMGSLNQDGLSNDNIQIGLQYIEHIERTLNHGSLTSREVTVLREIEM
                     LENMDLLSNYQLEELLDKIEVCAFNVEHAQLQVPESLRTCPVTLCEPEDGVFMRNS"
BASE COUNT          197 a          136 c           78 g          200 t
ORIGIN      
        1 gaattcctca taaatacccc atcttctggt tcacataatg taacagggca tgttcgtaag
       61 ctctctggca cttgcaattg tgcatgctcc acattaaatg cacatacttc aattttatct
      121 aacaactcct ctaactggta gttagaaagc aaatccatat tttcgagcat ctcaatttca
      181 cgcagtactg taacttcacg gcttgtcaaa ctaccatgat taagtgtacg ttcaatatgt
      241 tctatatatt gaaggccaat ctggatatta tcattcgata aaccatcctg gttaagactt
      301 cccataaaat atccatttac ctgcataggt cgtattgaca ccatcaactc acccagtctt
      361 atttgagctt ctccacgaat agtctgatta cgtatctcag acaaggtttc ttgaggaatg
      421 tcagacaaaa aactaacccc aggtaaaata taaggcgtta aatctacagg cattcctctc
      481 cctccatttt aataacttaa ttttgcttta gtaaaaaata aaagtacata aaactacatt
      541 tatatcatta attatcactt acaacaaaaa gcttctcttt ttgtatcaaa gtgacattca
      601 tcttttctca t
//
LOCUS       DAZGJW010000588          610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZGJW010000588 DAZGJW010000000
VERSION     DAZGJW010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.594"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..292
                     /locus_tag="ACHKIK_005804"
     CDS             <1..292
                     /locus_tag="ACHKIK_005804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HFO9086684.1"
                     /translation="LGIEKIEKLRGVLHNDRYDYNHESINRIQHIRSHEVQQLQLTDL
                     FIGALGYVHRGMNSNAGKIQVINRIKSHTNRELLKSTLPTESKFNIFVWEAR"
     gene            292..567
                     /locus_tag="ACHKIK_005805"
     CDS             292..567
                     /locus_tag="ACHKIK_005805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086685.1"
                     /translation="MLQMPDLLYFNGSWQEYIDDVYDVVREDILISNITFKGLPVRLR
                     YSPEYDGKEFGFWHLVSEGKIEEERIPDLQETARIRAVLTDIYSKRS"
BASE COUNT          213 a           96 c          131 g          170 t
ORIGIN      
        1 tttaggtatt gaaaaaattg agaaattaag aggagttctt cacaatgatc gctatgatta
       61 caatcatgag tcgataaaca gaattcaaca tatacgatct catgaagtgc aacaattgca
      121 gctaacggat cttttcattg gtgcgttagg ctacgttcat cgaggaatga atagcaacgc
      181 gggaaaaatc caagtcataa acaggataaa atcacataca aacagggagt tactaaaaag
      241 cactcttcca acagaaagta aatttaatat tttcgtgtgg gaggctcgct gatgcttcaa
      301 atgccagatt tattgtactt caatggaagt tggcaagagt atatagacga tgtatatgat
      361 gttgttagag aagatatttt aatctctaat ataacgttta agggtctccc tgttcgatta
      421 cgttattcac cggaatatga tgggaaagag ttcggatttt ggcatttagt atcagaggga
      481 aaaatagaag aagaacgaat acctgatctt caagaaacag cccgtatacg ggctgtttta
      541 acagatatct acagtaagcg gtcctaaact gtgggtatct tcaaagatca gactatcagg
      601 gagctaaagc
//
LOCUS       DAZGJW010000589          609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZGJW010000589 DAZGJW010000000
VERSION     DAZGJW010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.595"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..142
                     /locus_tag="ACHKIK_005806"
     CDS             <1..142
                     /locus_tag="ACHKIK_005806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO9086686.1"
                     /translation="RIGQEAVEEIESNHNRYRWTVEECRAIKAEYQQKLKKLRNSRSE
                     VA"
     gene            139..>609
                     /locus_tag="ACHKIK_005807"
     CDS             139..>609
                     /locus_tag="ACHKIK_005807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFO9086687.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQ"
BASE COUNT          197 a          120 c          154 g          138 t
ORIGIN      
        1 ccgcatcggg caggaagcag tagaggaaat cgaatcaaac cataaccgct atcgctggac
       61 tgtcgaagag tgcagggcca tcaaggcgga gtatcaacag aaacttaaaa aactgcgaaa
      121 cagcagaagt gaggttgcat gaatatctac gaaagaattg atggcagcaa ataccgaaat
      181 atttgggtag ttggcgatct gcacggatgc tacacgaacc tgatgaaaaa actggagacg
      241 ataggattcg acaccaaaaa agacctgctt atctcggtgg gcgatttggt tgatcgcggt
      301 acagagaacg tagaatgcct ggaattaatc acattcccct ggttcagagc tgtacgtgga
      361 aaccatgagc aaatgatgat tgatggctta tcagagcgtg gaaacgtcaa tcactggctg
      421 cttaatggcg gtggctggtt ctttaatctc gattacgaca aagaaattct ggctaaagct
      481 cttgcccata aagcagatga acttccgtta atcatcgaac tggtgagtaa aggaaaaaaa
      541 tatgtcatct gccacgccga ttatccttgt gataaatacg agtttggaaa gccagttgat
      601 catcagcag
//
LOCUS       DAZGJW010000590          607 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZGJW010000590 DAZGJW010000000
VERSION     DAZGJW010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.596"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..419
                     /gene="cesT"
                     /locus_tag="ACHKIK_005808"
     CDS             <1..419
                     /gene="cesT"
                     /locus_tag="ACHKIK_005808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HFO9086688.1"
                     /translation="GSISFNENRLCSFAIDEIYYISLSDANDEYMMIYGVCGKFPTDN
                     PNFALEILNANLWFAENGGPYLCYESGAQSLLLALRFPLDDATPEKLENEIEVVVKSM
                     ENLYLVLHNQGITLENEHMKIEEISSSDNKHYYAGR"
BASE COUNT          198 a           88 c          117 g          204 t
ORIGIN      
        1 ttggatctat ttcatttaat gaaaatagat tgtgttcttt tgctattgat gagatttatt
       61 atatttcgtt atctgatgct aatgacgaat atatgatgat ctatggcgtc tgtgggaaat
      121 tcccgacaga taatcctaac ttcgctcttg agattttgaa tgcaaactta tggtttgcag
      181 agaatggtgg accatacctg tgttatgaat caggagcaca atcgctgttg ttagcgttac
      241 gttttccgct cgatgatgct acacctgaaa aacttgagaa tgaaatagaa gtcgtcgtta
      301 agtcaatgga aaacctgtat ttggtattac ataatcaggg aataacatta gaaaacgaac
      361 atatgaaaat agaggaaatt agttcaagcg ataataaaca ttattacgcc ggaagataaa
      421 agattattta ttaatataat ttactattca ttctaactca ttgtggtgga gcccataaca
      481 tgattactca tggtttttat gcccggaccc ggcacaagca taagctaaaa aaaacattta
      541 ttatgcttag tgctggttta ggattgtttt tttatgttaa ccagaattca tttgcaaatg
      601 gtgaaaa
//
LOCUS       DAZGJW010000591          602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZGJW010000591 DAZGJW010000000
VERSION     DAZGJW010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.597"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>602
                     /locus_tag="ACHKIK_005809"
     CDS             <1..>602
                     /locus_tag="ACHKIK_005809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO9086689.1"
                     /translation="NQSATDTLSSPVPETDAVETAIPGNEKTECKVEVEPSVEREGPF
                     YFLFTDKDGEKYGRANKLSGLEKALALGATEITKEEYFARKNGTYSGSQQNTGASDTT
                     AQPEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIQTRD
                     SVNQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQE"
BASE COUNT          206 a          150 c          147 g           99 t
ORIGIN      
        1 aaatcagagc gcaactgata ctctctcgtc gccagtacca gaaactgatg cagtggaaac
       61 ggcaattccg ggcaacgaaa aaaccgaatg caaagtggaa gtcgaaccat ctgtagagcg
      121 tgaggggccg ttctacttcc tcttcaccga caaggatggc gaaaaatacg gtcgcgcaaa
      181 caaactttct ggtctggaaa aagcactagc cttgggagct acggaaatca caaaagagga
      241 atacttcgca cgtaaaaacg gcacgtactc aggttcacaa caaaatactg gtgcatctga
      301 cacgaccgca caaccagagc cggtaaaagt taccgctgac gaagtaaaca aaattatgca
      361 ggcagccaat atcagccagc ctgacgccga taagttgctt gctgcctctc gcggagaatt
      421 tgttgcaggg attagcgacc cgaatgatcc gaaatgggtt aaggggatcc agacccgcga
      481 ttctgtaaac cagaaccagc atgaatcgga acggaactac caaaaagcgg aacaaaacag
      541 cccaaatgcg ttacaaaacg agccagaaac gaaacagcct gaaccagtgg cgcaacagga
      601 ag
//
LOCUS       DAZGJW010000592          602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZGJW010000592 DAZGJW010000000
VERSION     DAZGJW010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.598"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            44..>602
                     /locus_tag="ACHKIK_005810"
     CDS             44..>602
                     /locus_tag="ACHKIK_005810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HFO9086690.1"
                     /translation="MNTNIGMRYIDVVANGNAGEFYFAYWISSNFIWPCRILDIDMGL
                     DAQVEVYDDQNHSTGMFIGVQVKTTATTLEENPNVSVPMKNIAYWESINDPVVIIRTC
                     LNNNSKEPSLYWKHLPKSELRKYLNRAEEKGYETVTIMFEEDKNSLLKTDKSAWLKIF
                     LSDYDKEIIESVVGIKSKLDKLGKYL"
BASE COUNT          219 a           77 c          133 g          173 t
ORIGIN      
        1 aatatacaaa ggggaaatag tatttagccg tgggggagta ttaatgaata caaatatcgg
       61 tatgagatat atagacgtag tggcaaatgg taatgccgga gagttttatt ttgcatactg
      121 gatatcaagt aatttcatct ggccttgccg tattcttgat attgatatgg ggttagatgc
      181 tcaagttgaa gtttatgatg accaaaatca ttcaactgga atgtttattg gtgtgcaggt
      241 gaaaacaacc gcgactacgc tagaagaaaa ccctaatgtt tctgtaccca tgaaaaatat
      301 agcttattgg gaaagcataa atgatccagt tgttattatt cggacctgtc ttaataacaa
      361 ttcaaaagag cctagcttgt actggaagca tcttccgaaa agtgaattaa gaaaatattt
      421 aaatagagcc gaggaaaaag gttatgaaac agtaaccata atgttcgagg aggataagaa
      481 ctcattgctg aaaaccgata agtcagcatg gctaaagata tttttgagcg attatgataa
      541 ggaaatcatt gaaagtgtag tagggattaa aagtaaacta gataagttag gtaaatactt
      601 aa
//
LOCUS       DAZGJW010000593          600 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZGJW010000593 DAZGJW010000000
VERSION     DAZGJW010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.599"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>600
                     /locus_tag="ACHKIK_005811"
     CDS             <1..>600
                     /locus_tag="ACHKIK_005811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024231387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO9086691.1"
                     /translation="IKKSGIPVNLVKISDEDNQIVAVRGSSQSGIYYEVDIETGYEIL
                     SRRVYRTEYNNEIFWIRNGGLKGGQPFDFENLDIPTFFVDKPYSELASSPELSFINDD
                     SPLLFPYVDSRLPKPTSEMDISYYSSNFSSFAENTVTLMRGATEEEAWNIAYYKTAGG
                     SNKELEEIFIGGGPQANLSFTEYTSNIRSADAASRRHFLV"
BASE COUNT          200 a          107 c          128 g          165 t
ORIGIN      
        1 tataaaaaaa tccgggatcc ctgtcaatct tgtaaaaatt agtgatgaag acaatcaaat
       61 tgttgctgtc aggggaagtt ctcagagtgg catctactat gaagtggata ttgaaacagg
      121 gtatgagatt ttatcccgaa gagtttaccg tacagaatat aataatgaaa tattctggat
      181 acgaaatggc ggactgaaag ggggacaacc attcgatttt gaaaatctcg atatccctac
      241 ttttttcgta gataaacctt attccgaact ggcatcgtct ccggaactgt cctttatcaa
      301 tgatgacagc ccactattgt ttccttatgt ggactcaaga ttaccgaaac caacgtcaga
      361 aatggatatc agttattact catctaattt ttccagcttt gctgagaata ctgtaaccct
      421 gatgcggggg gctactgaag aagaagcatg gaatattgcc tattataaaa cagcaggagg
      481 aagcaacaaa gaactggaag agatttttat aggtggagga ccacaggcta atttaagttt
      541 cacggaatac acatcaaaca tcagaagtgc agatgcagca agcagaagac attttcttgt
//
LOCUS       DAZGJW010000594          595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZGJW010000594 DAZGJW010000000
VERSION     DAZGJW010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.600"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..554
                     /locus_tag="ACHKIK_005812"
     CDS             3..554
                     /locus_tag="ACHKIK_005812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO9086692.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
BASE COUNT          175 a          132 c          134 g          154 t
ORIGIN      
        1 ttatgtctgc ttttaaaaag tcacttcttg ttgctggcgt agcaatgatt ctttccaata
       61 acgtatttgc tgacgaaggt cacggcattg ttaaatttaa aggtgaagtt atctctgctc
      121 catgttcaat taaaccaggt gacgaagact taactgtaaa tctgggtgaa gtagcagata
      181 ccgttctgaa aagcgaccag aaatctttgg ctgagccttt cactattcac ctgcaagact
      241 gcatgttgag ccagggtgga accacttatt caaaagcgaa ggttactttc accacagcga
      301 atactatgac gggtcagact gacctgctga aaaacaccaa agaaactgaa atcggtggtg
      361 ccacaggcgt tggcgtacgt attctggata gccagagcgg cgaagtgact ttaggtactc
      421 cggtagtaat tacattcaac aatactaaca gctatcagga actgaacttc aaagctcgta
      481 tggaatcccc aagcaaagat gctaccccgg gcaacgttta cgctcaggct gactataaga
      541 ttgcttacga gtaatcacat taggccgggt tcgcccggcc acatcattaa cggat
//
LOCUS       DAZGJW010000595          593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZGJW010000595 DAZGJW010000000
VERSION     DAZGJW010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.601"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..168
                     /locus_tag="ACHKIK_005813"
     CDS             <1..168
                     /locus_tag="ACHKIK_005813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO9086693.1"
                     /translation="RAVSADPSTGTIAVKDMAITVTGVKAGKVSIPVISGNGQFATVA
                     EVTVTEAGAAG"
     gene            182..>593
                     /locus_tag="ACHKIK_005814"
     CDS             182..>593
                     /locus_tag="ACHKIK_005814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO9086694.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTESPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGAVHVDTDITEVAKNNALTDDDFSAG"
BASE COUNT          141 a          136 c          184 g          132 t
ORIGIN      
        1 agagcggttt ccgccgatcc gtcaacggga accattgctg tgaaagatat ggcgatcact
       61 gtgacggggg ttaaggctgg aaaagtgagt atccccgtga tttccggtaa tggtcagttt
      121 gccacggtag ctgaagtcac cgttactgaa gcgggcgctg cagggtaaac ggaggtaata
      181 catgtttctg aaaaccgaac aatttgaata taacggtgtg tccgtcacgc tttccgagct
      241 gtctgcgctg cagcgtatcg agcatcttgc cctgctgaaa cgacgggcag aacaggctga
      301 agccagcggt aacctgcagg tgagcgtgga agaccttgtc agaaccggcg cgtttctggt
      361 ggcgatgtcc ctgtggcata accatccaca gaaaacggag tcaccatcaa tgaatgaggc
      421 tgtgatgcag atcgaacagg aggtgctcac cacctggcct gctgatgcca ttgcccgggc
      481 ggaagacgtg gtgttgcgtc tgtccgggat gagcggggct gttcatgtgg atacggatat
      541 caccgaagtg gcgaaaaata acgcgcttac tgatgatgat ttttctgcgg gaa
//
LOCUS       DAZGJW010000596          592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZGJW010000596 DAZGJW010000000
VERSION     DAZGJW010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.602"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            195..383
                     /locus_tag="ACHKIK_005815"
     CDS             195..383
                     /locus_tag="ACHKIK_005815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9086695.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            441..>592
                     /locus_tag="ACHKIK_005816"
     CDS             441..>592
                     /locus_tag="ACHKIK_005816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086696.1"
                     /translation="MKIEYTPERGRGFVRPGETEKQQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCV"
BASE COUNT          182 a          140 c          130 g          140 t
ORIGIN      
        1 gagaaaaaag gcatgaaaca atattttatg cctttgtatt aacatgaatt taaatgattt
       61 tatcctgatt attcctcttt ctgtaaacca tacggcctca acatttgcca actctgaatc
      121 atcctgacac aatcagacac atcccaacgc aatagaacac gaaataactc tttaagaaac
      181 gaaagggggc aatagtgtta agcactgatc ggtttatacg tgaaaaagaa tgcgaaaagc
      241 taacaggcct tagccgctca tgccgctacc gcctggaaaa ggccggacaa ttcccatcac
      301 gtcgtaaact tggcggtcgt tccgttggct ggtctttatc cgaggttctg gcctggaagg
      361 atagctgcaa ggcagttcat taatcacgct ggcggcacgc agccgccaca catcaatcat
      421 ctgaacacag agccataacc atgaagattg aatatacgcc agaacgtggg cggggattcg
      481 ttcgccctgg tgaaactgaa aaacaacaaa attggggttt ttcaggtata aaaaaagcgg
      541 ccccgaaatg gagccgcctt tctgaacaga taacccgctg cgccgtttgt gt
//
LOCUS       DAZGJW010000597          591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZGJW010000597 DAZGJW010000000
VERSION     DAZGJW010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.603"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..566
                     /gene="fimA"
                     /locus_tag="ACHKIK_005817"
     CDS             3..566
                     /gene="fimA"
                     /locus_tag="ACHKIK_005817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO9086697.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT          143 a          122 c          150 g          176 t
ORIGIN      
        1 ttatgaaact caaacatgtt ggtatgattg tcgtttctgt gttggcgatg tcgtctgctg
       61 cggtaagcgc agccgagggt gatgaatcag taacgaccac tgttaatggc ggtgttattc
      121 attttaaagg tgaagtggta aatgccgctt gtgcaattga ttcagaatca atgaaccaaa
      181 cggttgagct gggtcaggtt cgttcttctc gcttggctaa agcgggtgac ctcagctccg
      241 ccgttggctt caatatcaag ctgaatgatt gtgataccaa tgtttccagt aatgcagctg
      301 ttgcattcct gggtactact gtcaccagta atgacgatac gttagcgctg cagagttcag
      361 cggcaggctc tgcccaaaat gtcggtattc aaattttgga ccgtacgggt gaggtattaa
      421 tacttgatgg ggccactttt agtgctaaaa ccgacttgat tgatggcacg aatatactgc
      481 cattccaggc tcgttatatt gctctcgggc agtccgtagc tggtactgca aacgctgatg
      541 cgaccttcaa agttcaatat ctataattca atcttaaggt ggctatacgt c
//
LOCUS       DAZGJW010000598          586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZGJW010000598 DAZGJW010000000
VERSION     DAZGJW010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.604"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>586
                     /locus_tag="ACHKIK_005818"
     CDS             <1..>586
                     /locus_tag="ACHKIK_005818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9086698.1"
                     /translation="WKNLHFYARKVMSMWRYRITRLLSRKYPDLVIPDALAAEGSSKR
                     DWNRFLDSHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLVMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEDVVYVITE
                     TVRKTAMQIRWRGMYQRLLKVDPLKCILCGGQMR"
BASE COUNT          142 a          119 c          191 g          134 t
ORIGIN      
        1 cctggaaaaa ccttcatttt tacgcccgta aggtgatgag tatgtggcgt taccggataa
       61 cgcggttact gtcacggaaa tatccggacc tggtgatacc ggatgcgctg gcagcagaag
      121 gaagcagtaa acgggactgg aatcgcttcc tggacagtca ttaccggcgg ggctggaatg
      181 tcaacgtatc ccgggtgatg gataacgcca cacatgtggc ggtgtacttc ggctcttacc
      241 tgaaaaaacc gccggtgccg atgagccgtc tggagcacta tgctggtcag gatgaaattg
      301 gtctgcgtta caacagtcac cggacaaaac gggaagaata cctggtgatg agtggtgatg
      361 agtttatgga aaggttctcc tggcatgtgg cggataaggg gttccgtatg gtgaggtact
      421 acggtttcct gagtccggtg aagcgccggt tactggaaga tgttgtgtac gtcataacgg
      481 agacggtgag aaagacggcg atgcaaatca ggtggagagg gatgtatcag cggttactga
      541 aggttgaccc gctgaagtgc atcctgtgcg gaggtcagat gcgttt
//
LOCUS       DAZGJW010000599          585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZGJW010000599 DAZGJW010000000
VERSION     DAZGJW010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.605"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            112..498
                     /locus_tag="ACHKIK_005819"
                     /pseudo
     CDS             112..498
                     /locus_tag="ACHKIK_005819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          187 a           90 c          114 g          194 t
ORIGIN      
        1 ttgttgatga tttttatttt ccatgataga tttaaaataa cataccgtca gtatgtttat
       61 ggtatcatga tgatgtggtc gtgacaatct taagaacatt taggttattt tatgtatatt
      121 gaacagcatt ctcgctatca aaataaagct aataacatcc aattaagata tgatgataag
      181 cagtttcata caacggttat caaagatgtt ctattatgga ttgaacataa tttagatcag
      241 tctttactgc ttgatgatgt ggcgaataaa gcgggttata ccaagtggta ttttcagcgg
      301 ctgttcaaaa aagtaacagg ggtcacactg gctagctata ttcgtgctcg tcgtttgacg
      361 aaagcggctg ttgagttgag gttgacgaaa aaaactatcc ttgagatcgc attaaaatat
      421 caatttgatt cccaacaatc ttttacacgt cgatttaagt acatttttaa ggttacacca
      481 agttattatc ggcgtaataa attatgggaa ttggaggcaa tgcactgaga gatcccctca
      541 taatttcccc aaagcgtaac catgtgtgaa taaattttga gctag
//
LOCUS       DAZGJW010000600          584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZGJW010000600 DAZGJW010000000
VERSION     DAZGJW010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.606"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>584
                     /locus_tag="ACHKIK_005820"
     CDS             <1..>584
                     /locus_tag="ACHKIK_005820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086699.1"
                     /translation="NQNTRDIRNIKNFSSHQYSLGDMFDELINIIDKEQVLSTEQRKV
                     IFRRYEDLYVKLMHYSVFTDKTHQIIKQKYFNDIVPMILALDIRNTYRPDNEMAFYYH
                     IHSFLTQIPDNEDDIYHAARTYLRNYVKLCLSGYTPANAHFKDIFDGVYEFIRNIRKN
                     STPGKTKLIATINTCKETCKHLLYLSNEDKEKIIS"
BASE COUNT          226 a          104 c           86 g          168 t
ORIGIN      
        1 aaccagaata cccgtgacat ccgaaatatt aaaaattttt cctcacatca atattcactg
       61 ggcgacatgt ttgatgaatt aataaacatt attgataaag aacaagtatt gagtacagaa
      121 caacgaaaag ttatatttag gagatatgaa gatctctatg ttaagctaat gcactattct
      181 gtttttacag acaaaacaca tcaaataata aaacaaaaat attttaatga cattgtacca
      241 atgattctcg cactcgacat caggaacaca tatcgcccgg ataatgagat ggcattttac
      301 tatcatattc attcttttct cactcagata ccggataatg aggatgatat atatcatgct
      361 gcaaggacat atctgcgaaa ttacgttaag ttatgtttat ccggatacac accagcgaat
      421 gcgcatttca aagatatctt tgatggcgta tatgaattca ttcgtaatat tcgcaaaaac
      481 agtacaccag gaaaaacaaa acttatcgca actatcaaca catgcaaaga aacctgtaaa
      541 catctgcttt atttaagtaa tgaagacaag gaaaaaataa tttc
//
LOCUS       DAZGJW010000601          583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZGJW010000601 DAZGJW010000000
VERSION     DAZGJW010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.607"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..263)
                     /gene="yhaB"
                     /locus_tag="ACHKIK_005821"
     CDS             complement(<1..263)
                     /gene="yhaB"
                     /locus_tag="ACHKIK_005821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFO9086700.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSP"
     gene            complement(519..>583)
                     /locus_tag="ACHKIK_005822"
     CDS             complement(519..>583)
                     /locus_tag="ACHKIK_005822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFO9086701.1"
                     /translation="INVNFFSSDRTSFCECNRFP"
BASE COUNT          171 a          116 c          114 g          182 t
ORIGIN      
        1 aatggactca tattttccgg ttggattcgg atcattacag aaggaacatc gccttgatag
       61 aaattcaatg cagtaattgc agataatact ctccgcagtt cattacttat actgtaaatt
      121 ttattaacgt cattggtgga gacaatttct gcaaactttc tttttaccgt ccaataatca
      181 atatttctat tgtgattgtt aaccactgcg tgcattggcg ggattgaagg gtgaaaaata
      241 tgtgcaattg ggaagccttt cattgatgcc tgccttgtcg tcatgacaaa caataataga
      301 tgcttatttt cctcgcaggg ttctttcgca ctatcaaaaa acgctcattt acccggcgtt
      361 acagtaaacg aagcaacatt ctgtggccta cgacgatcaa aagctctgtg aaaatttccc
      421 ttgttgtctt tgtctgacct tatcgcggcg accagcaatt attcgttttg tgatcctcaa
      481 cacaaaatgc tattcgtcgc ctatgagggg agaagaattt atggaaaacg attgcattca
      541 caaaatgacg tcctgtcaga agagaaaaag ttgacgttaa tca
//
LOCUS       DAZGJW010000602          583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZGJW010000602 DAZGJW010000000
VERSION     DAZGJW010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.608"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>583
                     /locus_tag="ACHKIK_005823"
     CDS             <1..>583
                     /locus_tag="ACHKIK_005823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO9086702.1"
                     /translation="ALDRLVQQGGFDGRTQRYHYDLTGKLTQSEDEGLVTLWYYDESD
                     RITHRTVNGEPAEQWQYDDHGWLTDISHLSEGHRVAVHYGYDDKGRLTGERQTVENPE
                     TGELLWQHETTHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGDTPLLEYTRDR
                     MHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQSQ"
BASE COUNT          138 a          172 c          190 g           83 t
ORIGIN      
        1 gcgctggacc ggttggtaca gcagggcggc tttgacgggc ggacgcaacg ttatcattat
       61 gacctgaccg gaaaactcac gcagagtgaa gatgaggggc ttgtcaccct ctggtactac
      121 gatgaatcgg accgtatcac tcaccgcacg gtgaacggcg aaccggcaga gcagtggcag
      181 tatgatgacc acggctggct gacagacatc agccacctga gcgaaggcca ccgtgtcgct
      241 gtccactacg gctacgacga taagggccgc ctgaccggcg aacggcagac ggtggagaac
      301 ccggagacgg gggaactgct gtggcagcat gagacaacac acgcctacaa cgaacagggg
      361 ctggcaaacc gcgtcacgcc ggacagcctg ccgccggtgg agtggctgac ctacggcagc
      421 ggttatctgg cgggcatgaa gcttggcgac acgccgctgc tggagtacac gcgcgaccgc
      481 atgcaccggg agacggtgcg cagctttggc agcatggcag gcagtaatgc cgcatataaa
      541 ctgaccagca catacacccc cgcaggccag ttacagagcc agc
//
LOCUS       DAZGJW010000603          581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZGJW010000603 DAZGJW010000000
VERSION     DAZGJW010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.609"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>581
                     /locus_tag="ACHKIK_005824"
     CDS             <1..>581
                     /locus_tag="ACHKIK_005824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFO9086703.1"
                     /translation="SNKIGQRVYTVVSEMSIDAWITKEPQPFVYRRSGAYQKLTIGSD
                     WGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGITNKASSYGVP
                     PDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIATCNQLLKSLYY
                     DVEVLFDWINDGQKFESIHPKGIIPEKIITKRS"
BASE COUNT          164 a          110 c          134 g          173 t
ORIGIN      
        1 tgtcgaataa aataggtcag cgtgtttata ctgttgtctc tgaaatgtcg attgatgcat
       61 ggataacaaa agaaccgcag ccttttgttt atcgccgtag cggcgcttac cagaaactca
      121 ccattggctc agactggggt caactgtttg actgtgcctg gatgcgagtt tatggtaaac
      181 gtccagcaga taaatttaga cacaaactcg tcgctctcgt tgatatcggt ggcgaaggtc
      241 ttattgtgga taagaatggt tcacccatct gcggcatcac gaataaagca tcatcttatg
      301 gcgtaccgcc agataaacca ggtaaatggg tggttgacct ttctctcgtt agtgaaaatg
      361 acgaggtcga gttttggatt gatgctgcgt gcaacgattt atttggctat gtcactaatg
      421 gagggattat tactgatgtt catatcgcga catgtaatca actactgaaa tccctttatt
      481 atgatgttga agtgcttttc gactggatta atgatggtca gaagtttgaa tccatacatc
      541 ctaaaggtat tatcccagaa aaaattatca ccaaaagaag c
//
LOCUS       DAZGJW010000604          580 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZGJW010000604 DAZGJW010000000
VERSION     DAZGJW010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.610"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..580
                     /locus_tag="ACHKIK_005825"
                     /pseudo
     CDS             1..580
                     /locus_tag="ACHKIK_005825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          169 a          134 c          157 g          120 t
ORIGIN      
        1 ctgcgtaagg cgttaaatga agctcgcctt gagcgagata tattaaaaaa agcaacagcg
       61 tattttgcac aggagtcgct gaaaaatacg cgttaatcga acaatggcga caacaatttc
      121 ccattgaagc gatgtgtcag gtatttggtg tatccaggag cggttattac aactgggtac
      181 agcatgaacc ctcagacaga aaacaaagtg atgagcggct aaaactggag attaaggtgg
      241 cacatatccg cactcgcgaa acatatggaa cccggcggct ccagacggag ctggcagaga
      301 atggcatcat cgttggtcgt gaccgactgg cacgtcttcg taaggagcta aggctacgct
      361 gtaagcagaa acgcaagttc agagcgacta cgaactcgaa ccacaatctg ccagttgcgc
      421 caaatctgct gaaccagacg ttcgctccta cagcaccaaa tcaggtctgg gtggcggacc
      481 tgacgtatgt tgccacacag gagggatggt tgtacctcgc tggcatcaaa gatgtttata
      541 cgtgcgaaat tgtcggctac gccatgggag agcgcatgac
//
LOCUS       DAZGJW010000605          578 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZGJW010000605 DAZGJW010000000
VERSION     DAZGJW010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.611"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..>578
                     /gene="rfaY"
                     /locus_tag="ACHKIK_005826"
     CDS             3..>578
                     /gene="rfaY"
                     /locus_tag="ACHKIK_005826"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFO9086704.1"
                     /translation="MKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDDTKVILIDTA
                     RGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSINDYFLLAERKT
                     LNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGDPHKGNFIVS
                     EKGLRLIDLSGKKTTAVLKAKDRIDLERHYNI"
BASE COUNT          202 a           83 c          104 g          189 t
ORIGIN      
        1 ggttgaaagt tttcataaaa gataatgatc ccttttatga gcaagttcta aatgactttt
       61 taacttgcag ggttaaaaca ttaaaagttt ttcgttcgat tgatgatact aaagtcattc
      121 ttattgacac tgctcgtgga cctcttgtac ttaaagttta tgcgccaaag cataaaatga
      181 ccgaacgttt cttaaaatct tgcataaaaa aagattatta tgaaaattta atttatcaaa
      241 ccgatcgggt acgtggtgaa ggcatccaat caattaatga ctattttttg ctagctgaac
      301 gtaaaacatt aaatttcgct cattattata ttatgctcat tgagtatatt gaaggtgtcg
      361 ggttaaatga gtatttggaa atttctgaag atctgaagga tcaattatca gaatcgataa
      421 aagagttgca tcagcatgga atggtgtctg gcgatcctca taaaggaaat tttatagtat
      481 ccgaaaaagg tttaagacta attgaccttt caggtaaaaa aacaaccgct gttttaaaag
      541 caaaagatcg cattgatctt gaacggcact ataatata
//
LOCUS       DAZGJW010000606          577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZGJW010000606 DAZGJW010000000
VERSION     DAZGJW010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.612"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..186
                     /locus_tag="ACHKIK_005827"
     CDS             <1..186
                     /locus_tag="ACHKIK_005827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO9086705.1"
                     /translation="TSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGEC
                     LPLKKVSLYLNNETPAG"
     gene            282..>577
                     /locus_tag="ACHKIK_005828"
     CDS             282..>577
                     /locus_tag="ACHKIK_005828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO9086706.1"
                     /translation="MSKVFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEASASFVPVETES"
BASE COUNT          165 a          133 c          134 g          145 t
ORIGIN      
        1 acttcagcat ttgcactgat gatgacggtt tttcttataa cgggtgaatc acagaatgtg
       61 attaccggaa tttatgcaag taaagaatcc tgcctccagg caagagacga gcaaaaaatt
      121 tctggtgaat gcctcccgct aaaaaaagta tcgctgtacc tgaataacga aacaccggct
      181 ggataatcct ccagccatat taacaccata ccaacggatt aaaaatgcca gcaatggcag
      241 ggattcgttc accctgaaat ctgtaatgag gttaaaacaa aatgagtaag gtctttattt
      301 gcgccgctat tcctgatgaa caggccataa aagaagatag cgctgttgcg gtggccactg
      361 ccattgaagc tggtgatgag cgtcgcgcac gcgcaaaatt tcattggcaa tttctggagc
      421 aattccctgc agctcaggac tgcgcttata aatttattgt ctgtgaggat aaacccggca
      481 taccccgccc tgccctcgat tcctgggatg ctgaatatat gcaggaaaac cgctgggatg
      541 aggcgtctgc ttcctttgtc ccggttgaga ctgaatc
//
LOCUS       DAZGJW010000607          574 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZGJW010000607 DAZGJW010000000
VERSION     DAZGJW010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.613"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>574
                     /locus_tag="ACHKIK_005829"
     CDS             <1..>574
                     /locus_tag="ACHKIK_005829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO9086707.1"
                     /translation="NKVLQNTILEFVHEINNISSARWMSKSEKNNKQKEAIEKFKKEV
                     SHMNGGQQFIWGFDKVIQEGLSGLIELSIDINDSTNHRDKSSLSPDGRAVLHFLGTIW
                     NMAMGAVPGYNALSGVSSILHSAIVKESSNICDYIQGAVRIGMDFVPGTRSDLHSRSL
                     QIKYEALKHIEKNINDNIIYHPSNNANFYSV"
BASE COUNT          191 a           76 c          121 g          186 t
ORIGIN      
        1 aataaagtgc ttcagaatac tattcttgag tttgtccatg aaattaataa tatatcatct
       61 gctcgctgga tgtcaaaatc agaaaagaat aataaacaaa aagaggcaat agaaaagttc
      121 aaaaaagaag tatcccatat gaatggcggg cagcagttta tttgggggtt tgataaggtt
      181 attcaagaag gcttaagtgg attgattgag ttaagtatcg atattaatga tagtacaaat
      241 catcgtgata agtcttctct ttctcctgat gggagagctg tgttacattt tttaggtaca
      301 atttggaata tggcgatggg agctgtacct ggttataatg cattgtctgg tgtaagtagc
      361 attttacata gtgctatagt aaaagaatct agcaatatct gtgattatat tcagggggct
      421 gtacgtattg gaatggactt tgttccaggc actcgctctg acttacatag ccgttcgctg
      481 cagataaaat atgaggcttt gaagcatata gaaaaaaaca ttaatgataa tattatttat
      541 catccgagta ataatgctaa tttctattct gtaa
//
LOCUS       DAZGJW010000608          569 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZGJW010000608 DAZGJW010000000
VERSION     DAZGJW010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.614"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..549)
                     /locus_tag="ACHKIK_005830"
     CDS             complement(<1..549)
                     /locus_tag="ACHKIK_005830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO9086708.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILENNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGV"
BASE COUNT          195 a          111 c           79 g          184 t
ORIGIN      
        1 tacaccagag ttaaaatagc taccgttaaa atcctcatta cacaaacgct cagcgctttt
       61 actttgcata gcatctacat caattaccac tgccccatat tcatctttaa actctaatgc
      121 tattaactcg ttcaatgaac ccttacagac gatatcagca tcaagatata ataatgaatc
      181 caatcgcgca gaaaaataat caaatgaaag caaacgataa taaatagata ctggccagat
      241 atttgattga ggtagcgcct tcaatggctc agaatcaata tgatataatt taattactgt
      301 ttgatacttc gttgctaatt gtgataataa ttcaatatac tctttgtcta agtaatcagc
      361 gattatgtga aaggtaaatt tgttttgttt attattttca agaattgaag caattgaaat
      421 cgcagcccca acctggtagt tctcatcaat accccatgag acattaaacg tttcccgttc
      481 atcaagttta acggggcgct tgtccagttc aattatatct ttaaattgag taagatgttt
      541 aaaatccaat ttaattaccc ttcatcata
//
LOCUS       DAZGJW010000609          568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZGJW010000609 DAZGJW010000000
VERSION     DAZGJW010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.615"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            22..240
                     /gene="ygeI"
                     /locus_tag="ACHKIK_005831"
     CDS             22..240
                     /gene="ygeI"
                     /locus_tag="ACHKIK_005831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeI"
                     /protein_id="HFO9086709.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMAPFQNDMYSEDNPISPYYKIEW"
     gene            323..>568
                     /locus_tag="ACHKIK_005832"
     CDS             323..>568
                     /locus_tag="ACHKIK_005832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO9086710.1"
                     /translation="MRSPKVKFLTIFTFCIFITKMSFASNSCSNEAGTMFRIEPNLIK
                     AIALVESNLKKDSIGKNRDKNNNIKSLDYWLMQINQMH"
BASE COUNT          220 a          100 c           84 g          164 t
ORIGIN      
        1 acaattaaaa aagggagctt tatgactaat ccaatcggta tcaataattt atctcaaagt
       61 tcaaatatag cgaatgcaac gggtgatgaa gtcgtatcat tagataaaca catcaacacc
      121 agtgctactg atacagatca aatacaagca tttatagttt caacatggat ggcgcctttt
      181 caaaatgata tgtattcaga agataatcct atctcacctt actataaaat tgaatggtag
      241 acgattgacc aaataaatca taacgagata cttttataat agtatctcgt ccctttcatt
      301 caatcctcac acatgaaaaa atatgcgctc accaaaagtt aaattcttaa ccatcttcac
      361 gttttgtata tttattacaa aaatgtcttt cgccagcaac tcttgctcta atgaagcagg
      421 aacaatgttc agaatcgaac caaatttaat taaagctatc gctcttgttg aaagcaatct
      481 aaaaaaggat agcataggca agaacagaga taagaataac aatataaaga gtttagatta
      541 ttggttaatg cagataaacc agatgcat
//
LOCUS       DAZGJW010000610          565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZGJW010000610 DAZGJW010000000
VERSION     DAZGJW010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.616"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            158..373
                     /locus_tag="ACHKIK_005833"
     CDS             158..373
                     /locus_tag="ACHKIK_005833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO9086711.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKTARGD"
     gene            378..>565
                     /locus_tag="ACHKIK_005834"
     CDS             378..>565
                     /locus_tag="ACHKIK_005834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFO9086712.1"
                     /translation="MKKKYELVVKEINNYPDKIAVTVALEIGGHPSLLLPHVAISLDR
                     TEGATLEFYEAEAKKQAK"
BASE COUNT          153 a          110 c          165 g          137 t
ORIGIN      
        1 catcataaaa agtgagccag tcaaatggaa ggtatcgtta aactcaccgg tagtgtcagt
       61 gggtcgtctg agacgcctgc atgagttatc agagccatca gtagttaact ggtggctttt
      121 ttattgttgt cagcttccgg ataacgggag acggggtatg taccagatgg aaaaaatcac
      181 aacaggtgtg tcatacacca cgtcagcggt agggacggga tactggttac tgcaactgct
      241 ggacaaagtc tctccgtccc agtgggtggc aataggtgta ctggggagtc tgctgtttgg
      301 cctgctgacg tatctgacta acctgtattt caaaatcagg gaggaccgcc gtaagacggc
      361 gcggggagac taaagcgatg aagaaaaaat acgaactggt tgttaaagag ataaataatt
      421 acccggataa gattgctgtt actgtggcac ttgaaattgg cgggcatccg tcgttgttgt
      481 tgccacatgt ggcgattagt cttgaccgta ctgaaggtgc cacgctggag ttttacgaag
      541 ctgaggcgaa aaagcaggcg aagca
//
LOCUS       DAZGJW010000611          563 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZGJW010000611 DAZGJW010000000
VERSION     DAZGJW010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.617"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(382..489)
                     /locus_tag="ACHKIK_005835"
     CDS             complement(382..489)
                     /locus_tag="ACHKIK_005835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO9086713.1"
                     /translation="MTLAQFAMIFWHDLAASILAGIITAAIVGWWRNRK"
BASE COUNT          123 a          153 c          136 g          151 t
ORIGIN      
        1 tgttctggtt caagattagc ccccgttctg ttgtcaggtt aaacctctca acgtgcgggg
       61 gttttctctt tccagcaacc aatgccacca gggataaagc ccccgcaaca ttgcgcctca
      121 ccggataacg ccggcttgat gtggatacta cgtctcaatt catcttcact tcatccctga
      181 aatgtttgca atgaagagtg cattccggtt tttcaacagc tgttacagtc gtttcatgag
      241 ggctctggat gatgcttcca gctcgggttg ccaatgttta ctggcggaag agataaagac
      301 aaaaaaggcc gcaggctgtt acccctgcgg ccggtttcgg gcgcatattg ccatcacggc
      361 agcctgacgc ccgttttcgc cttacttccg gttacgccac cagccgacaa tcgctgcggt
      421 aataattccc gccaggatcg atgctgccag gtcgtgccag aaaatcatgg caaactgcgc
      481 gagcgtcata tagccgcctt gttgtaatga caacattttg cggctattct tgaattgttg
      541 atggttcaag attagccccc gtt
//
LOCUS       DAZGJW010000612          558 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZGJW010000612 DAZGJW010000000
VERSION     DAZGJW010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.619"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>558
                     /locus_tag="ACHKIK_005836"
     CDS             <1..>558
                     /locus_tag="ACHKIK_005836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086714.1"
                     /translation="IRALGEEFSTVLADLQRTFEGKMAAQAQAFEEKLASLSAVLQKH
                     VTVDEVHPVLQAMVDDAVGTIPVPRDGRDYDPDVLQQAVNDAVANIPVPADGKSITPD
                     DVRPMLEQMVKEAVSHIPAPRDGRDYDPEVLKQAVNDAVANIPVPADGKSITPDDVRP
                     MLEQMVKEAVSHIPAPRDGRDYDPEV"
BASE COUNT          125 a          125 c          185 g          123 t
ORIGIN      
        1 attcgtgcac tgggcgaaga attctccacg gtgctggcgg atttacagcg cacatttgag
       61 gggaagatgg ccgcgcaggc acaagcgttt gaagagaaac tggcttccct gtcggcggta
      121 ttacagaagc atgtgacggt ggatgaggta catccggttc tgcaggcgat ggtggatgac
      181 gctgtgggga ccattccggt accgcgtgat ggtcgtgatt atgatccgga tgtactgcag
      241 caggcggtga atgatgcggt tgcgaatatt ccggtaccgg cggacggcaa aagtatcacc
      301 cccgatgatg tgcgtccgat gcttgagcag atggtgaaag aggctgtaag ccatatccct
      361 gctccgcgtg acggtcgtga ttatgatccg gaagtactga agcaggcggt gaatgatgcg
      421 gttgcgaata ttccggtacc ggcggacggc aaaagtatca cccccgatga tgtgcgtccg
      481 atgcttgagc agatggtgaa agaggccgta agccatatcc ctgctccgcg tgatggtcgt
      541 gattacgatc cggaagta
//
LOCUS       DAZGJW010000613          558 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZGJW010000613 DAZGJW010000000
VERSION     DAZGJW010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.620"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>558)
                     /locus_tag="ACHKIK_005837"
     CDS             complement(<1..>558)
                     /locus_tag="ACHKIK_005837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFO9086715.1"
                     /translation="SAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPEAYFRM
                     SPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGEIHKFRCVP
                     HLTGRRFEHGVTDCYTLFRDAYHLAEIEMPDFVREDDWWRNGQNLYLDNLEATGLYQV
                     PLSAAQPGDVLLCCFGSSVPNHAAIY"
BASE COUNT          130 a          198 c          138 g           92 t
ORIGIN      
        1 gtaaatagcg gcgtgattcg gcaccgatga accaaaacag cacagcagca catcgcccgg
       61 ctgcgccgct gacaacggca cctgatacag ccctgtggcc tccagattat ccagatagag
      121 attctgaccg ttacgccacc agtcatcctc acgcacaaaa tccggcatct caatctccgc
      181 cagatgataa gcatcccgga acagtgtgta acagtccgtc acaccgtgct caaagcgccg
      241 cccggtgaga tgcggcacac agcggaactt atgaatctca ccccggcaga ccagccacca
      301 cggcaaatca ctctgcacct gcagccgccg gtcggcctca ctcagccagg gcagaccacc
      361 ggggtggctg tggaccagtg ccacaatctc cccctgcatt tctgcctgca gccagtcttc
      421 cggcgacata cggaaatacg cctccggctc accggagata ttcacgcagg ggaaatatct
      481 ttccccctcc ggcgtgctta ccacgaagcc gcacgactcc gctggcgcac atcgccgggc
      541 gtgcgccaga atcgctga
//
LOCUS       DAZGJW010000614          554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZGJW010000614 DAZGJW010000000
VERSION     DAZGJW010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.621"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..496
                     /locus_tag="ACHKIK_005838"
     CDS             <1..496
                     /locus_tag="ACHKIK_005838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO9086716.1"
                     /translation="TIQNWQEQGMPVLRGGGKGNEVLYDSAAAIKWYAERDAEIENEK
                     LRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNARDSAEVVETAFCTFVLS
                     RIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMNKAAALDELIPGLLSEYI
                     EQSG"
BASE COUNT          139 a          135 c          169 g          111 t
ORIGIN      
        1 taccattcag aactggcagg agcagggaat gcccgttctg cggggtggtg gcaagggtaa
       61 tgaagtgctt tatgactctg ccgccgccat aaaatggtat gccgaaaggg atgctgaaat
      121 tgagaacgaa aagctgcgcc gggaagttga agaactgcgg caggccagcg agacagatct
      181 ccagccaggg actattgagt acgaacgcca tcgacttacg cgtgcgcagg ccgacgcaca
      241 ggagctgaaa aatgccagag actccgctga agtggtggaa accgcattct gtactttcgt
      301 gctgtcgcgg atcgcaggtg aaattgccag tattctcgac gggatccccc tgtcggtgca
      361 gcggcgtttt ccggaactgg aaaaccgaca tgttgatttc ctgaaacggg atatcatcaa
      421 agccatgaac aaagcagccg cgctggatga actgataccg gggttgctga gtgaatatat
      481 cgaacagtca ggttaacagg ctgcggcatt ttgtccgcgc cgggcttcgc tcactgttca
      541 ggccggagcc acag
//
LOCUS       DAZGJW010000615          552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZGJW010000615 DAZGJW010000000
VERSION     DAZGJW010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.622"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..86)
                     /gene="hokD"
                     /locus_tag="ACHKIK_005839"
     CDS             complement(<1..86)
                     /gene="hokD"
                     /locus_tag="ACHKIK_005839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO9086717.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDL"
     gene            complement(332..436)
                     /locus_tag="ACHKIK_005840"
     CDS             complement(332..436)
                     /locus_tag="ACHKIK_005840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086718.1"
                     /translation="MSDDISLAMEGALAVIAVVGVYCLVVFLMDRLGN"
BASE COUNT          150 a          152 c          105 g          145 t
ORIGIN      
        1 aggtctttcc tcgttaccag tgccgtcact atgacggtta aacagatgac gatcagggcg
       61 attaacatcg ccttttgctg cttcatagcc tgcttctcct tgcctttcgg cacgtaagag
      121 gctaacctac atttgtgaga catagattgg gcctcagatt aatgttaagc gtcctgcaag
      181 acgcgtaatg ttaactgggg cttttctcta tctgccgttg gtgttcatgc ccgaggcaga
      241 tagcctcaag cacccgcagc aattctactt aactctcgct ttaccgcaaa ccgtttttac
      301 ccgatatggg aattcccata tcgtaatgaa ttcagttccc tagtcgatcc atcaaaaaca
      361 caaccaggca gtaaacgccc acaacagcaa taacagccag cgcaccttcc attgccagtg
      421 aaatatcatc cgacatattc cctcctttgg tgtgaatccc ggcgaacgtt tttaccccca
      481 ccgacaaata acatatacta aaaaatcaat agctatagca acgcctgtaa ttgcaaaggc
      541 ttcaggccag at
//
LOCUS       DAZGJW010000616          550 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZGJW010000616 DAZGJW010000000
VERSION     DAZGJW010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.623"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..283)
                     /locus_tag="ACHKIK_005841"
     CDS             complement(<1..283)
                     /locus_tag="ACHKIK_005841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086719.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANATTYDTWTYYDNPTTALD
                     WSNMDAPGTVDGNYVNYSGFVYYNNTNGDFDTTFNGDTVNGTISTYYLNH"
BASE COUNT          178 a          101 c          106 g          165 t
ORIGIN      
        1 catgattcag gtaatatgta gagatagtcc cgttaacagt gtcaccatta aaggtggtat
       61 cgaaatcacc gtttgtgttg ttatagtaga caaagccact ataattcaca tagttaccat
      121 ctacagtacc cggtgcatcc atattgctcc agtcaagagc ggtagttgga ttgtcgtaat
      181 aagtccaggt gtcataagta gttgcattag cctgagaggt gatagccaga gtgcaagcca
      241 atgctaattg tgatactaca agtttctttt tccaggagtg catttgtcat ccctcctcag
      301 ggacgtaata agttgatcca tcaattatca atgcatagaa aacatattat gtttcgcgct
      361 aatgattatg ctgcgttaca gacgaatggt tcaattattc tttcccctaa gtccgaatgt
      421 ggacatatat aacctatgga gaaaaacacg catcaaattg ataaaagggc gatatttcaa
      481 catgttgcag ataatgttat ttttgataca tacgcgttat aaatatcaaa aacacaaacc
      541 agataaatat
//
LOCUS       DAZGJW010000617          543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZGJW010000617 DAZGJW010000000
VERSION     DAZGJW010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.624"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..459)
                     /gene="nleG"
                     /locus_tag="ACHKIK_005842"
     CDS             complement(<1..459)
                     /gene="nleG"
                     /locus_tag="ACHKIK_005842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HFO9086720.1"
                     /translation="MPVILNFSSERVLSESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAK"
BASE COUNT          159 a          115 c          108 g          161 t
ORIGIN      
        1 ttttgcattt ctgacaaaaa caccagtttc aggaatgcat aatgttatcg ggcaattcag
       61 gtgttgttca tgacaggaaa agtcttgatg actaacagta aatgcatgag agttaatttt
      121 gttttgtatt gcactactga cgttttggcg cgtacatggg gttgcacggc tctgttccat
      181 atataaacgg aatgcctgca aaaaagtatg gcctctgtta agctgtatct ccagattttc
      241 tgcgagaagg cgatgccctc ttgcactaag gcgatccaac agtccgccgg aaaccggttc
      301 cacgctaaaa ctgtccataa atgaaatatg atgaagtctc atagtgcggc ctctcacaac
      361 aagctgttca ctctgactaa ctcttccgac atgacgtaaa gcctccagtt cactttcaga
      421 caatactctt tcactcgaaa agtttaatat gacaggcata ttttctccct ctttagtcta
      481 tgttattgaa ccatattggt gacaggagtc aagatactaa atatgtgata ggattcaaac
      541 aaa
//
LOCUS       DAZGJW010000618          542 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZGJW010000618 DAZGJW010000000
VERSION     DAZGJW010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.625"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          168 a          119 c          108 g          147 t
ORIGIN      
        1 acttcagcta ccgtcgtttc atctctccca cgaaccggag ccgcaacttg ttgcatcaca
       61 gggctgacaa gtgaactgaa atccgcacct taatattctg actggctgcg tacattcatt
      121 gcatgaagcg gccggagcgg ctgagctgaa ggtccgttgt gagcgagaag cggaagtccg
      181 aaatggtgct tattactata ggggagtgat cgtctatcgg ggtggggtca gtctaattaa
      241 cgaccctgga gacgtccaga ttatccttag cgattcaaac ctttttacga gacccaaaga
      301 tttgatactc aaaatctcag acataaacca tctaaacata cggcttacta tcatgatgat
      361 aggggaacgg ctagcaaagg gtcaaattat cttgataact aataaaagtt ctttacatcc
      421 ataaaatcca tgatgtattt atctaatcct ttgcgattga aaaaaccgaa actttcaaag
      481 tcttttatta ttttccaatc actaaatacg tatgccaaac ataccaaatg aagaaatttc
      541 ac
//
LOCUS       DAZGJW010000619          542 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZGJW010000619 DAZGJW010000000
VERSION     DAZGJW010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.626"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(277..496)
                     /locus_tag="ACHKIK_005843"
                     /pseudo
     CDS             complement(277..496)
                     /locus_tag="ACHKIK_005843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          154 a           94 c          129 g          165 t
ORIGIN      
        1 agataactag actataagat gttagaacat attgactgat aagtgtaatt cacagacatc
       61 ttttcagttt tttctgttaa ttttctaatt gatggactgt atacagggat aaaaggagag
      121 ggtattgtct ggacatttct gttagcataa ttggaaatgt gcgtgaaaaa aagcataaca
      181 gacatcatgt gttatgtatg tattcagagt gcccataatt caccagtgca gggataaatt
      241 gtaaaaaaat cgcagtacgt cgtatgcaga acggtgcggc gtacgcagtt ccagtggacc
      301 gtcgcctgtg ataacggtct ttgtggaata accgttgcgg gagttagctc ctgatctgga
      361 ctgatttttc tcattcccaa gatggtgtgt catctctgca ttgagagcgg cttcaacgct
      421 gagctttttc agcaaccgat caaactgact gaggtcttct gagggggctg aggtttttgg
      481 ccagttcgtt agcaatacac cagaaaaatt ctaaacgagc ctgtacatag atttgtgtaa
      541 tt
//
LOCUS       DAZGJW010000620          537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZGJW010000620 DAZGJW010000000
VERSION     DAZGJW010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.627"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            243..>537
                     /locus_tag="ACHKIK_005844"
     CDS             243..>537
                     /locus_tag="ACHKIK_005844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086721.1"
                     /translation="MKVRSIGFTINNNNKNINTVDVMNAFINASNREHSRTDYTRKIL
                     ISDVNDFYYGLVVTFRNQKKNCKSQFVDGKFQLKIEDLQGSDKLANFNFFLIKK"
BASE COUNT          191 a          100 c           90 g          156 t
ORIGIN      
        1 tgacatacca ataactcttg tctaaaaagc cagcaagata aaaagccagt attcacgacc
       61 accagcgtgt ttactgtact gcacaaggtt tacaggtaca aaaaaaccgc tcagcggcgg
      121 gtttaagttg tgtggcgaag taaccactct taacacgata taatactttt tgcgtacgcg
      181 ttaggatttt tataaactat gcgtccccat tctcgctatc tttagtcggt cctggaatac
      241 acatgaaagt tagaagcata ggatttacaa taaataacaa taacaaaaat attaataccg
      301 ttgacgtaat gaatgctttt atcaacgcat caaacagaga acacagtcgc acagactata
      361 ctcgtaaaat tctcatttcg gatgtgaatg atttctatta tggattagtt gtcacattca
      421 gaaaccaaaa aaagaactgt aagtcgcaat ttgttgatgg taaattccag cttaaaattg
      481 aagatcttca aggaagtgac aaacttgcta atttcaactt tttcttaatt aaaaaat
//
LOCUS       DAZGJW010000621          532 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZGJW010000621 DAZGJW010000000
VERSION     DAZGJW010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.628"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            302..511
                     /gene="fumD"
                     /locus_tag="ACHKIK_005845"
     CDS             302..511
                     /gene="fumD"
                     /locus_tag="ACHKIK_005845"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO9086722.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          180 a           78 c          127 g          147 t
ORIGIN      
        1 ccaaaaggaa aacaaaaaca gcgtttcaaa aaatatacaa cttgttagac aataaattgt
       61 tatcaaagat ttaacgttcc agggaaaagg ttacgcaaaa acttcgctgt ttgcgcaacg
      121 tctggtgcca aagttgttag tgtgaggata agtgaaagtt catccggtgt ataaatatat
      181 catcgctcaa ttgctatagt taaaataatt tatcgcgtta tttgtctgat tttgtttgat
      241 ttttgtttaa ttggttagat tgatatatgc tgatagttgg catgataaat aggtataaag
      301 aatgggaaat cggacaaaag aggatgaact gtaccgagaa atgtgcagag ttgtcggtaa
      361 agttgtgctg gaaatgcgcg atctggggca ggaaccaaag catattgtta tcgcaggcgt
      421 attacgtaca gcattagcca ataaacgcat tcagcgcagt gagctggaaa agcaggcgat
      481 ggaaaccgtc attaacgcac tggtaaaatg accattcagg atgaggcggt aa
//
LOCUS       DAZGJW010000622          532 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZGJW010000622 DAZGJW010000000
VERSION     DAZGJW010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.629"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>532)
                     /locus_tag="ACHKIK_005846"
     CDS             complement(<1..>532)
                     /locus_tag="ACHKIK_005846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFO9086723.1"
                     /translation="TIPYVVSTLNISGPDMIMRQMKKYYKSLGRIGEVHIKDNKLSDM
                     NFVGVYASSDKENKSFNWLNPVSVGVNDITPDDESSWAVRNNDINKILFEKINCHVPE
                     KLPTSLYYEIDSRVFFHGWDNKSIQYVTEINKNLIKDINLLLTSSNVDVKLLIKLDRE
                     LYAISSKIENPLALRAI"
BASE COUNT          164 a           92 c           73 g          203 t
ORIGIN      
        1 tatagcgcgc aaggctaacg gattctctat tttgctgctt attgcatata attcacgatc
       61 tagctttatt aaaagtttaa catcaacatt agacgatgtt aacaataaat ttatatcttt
      121 aataaggttt ttattaatct cagtaacata ttgtatgctt ttattatccc atccatgaaa
      181 aaaaactcgc gagtcgattt catagtatag ggaggtaggt agtttttcgg gtacatgaca
      241 gtttattttc tcgaataaaa ttttattaat atcattattt cttacagccc atgagctttc
      301 atcatccggt gtaatatcat taacaccaac actcactgga ttcagccagt taaaactttt
      361 attttcttta tctgaacttg catatacacc cacaaaattc atatcactta atttattgtc
      421 tttaatgtgt acttcaccta tgcgtcctag agatttatag tattttttca tttgtcgcat
      481 aatcatgtct gggccagata tatttagagt acttactaca taaggaattg ta
//
LOCUS       DAZGJW010000623          529 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZGJW010000623 DAZGJW010000000
VERSION     DAZGJW010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.630"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            32..403
                     /gene="ler"
                     /locus_tag="ACHKIK_005847"
     CDS             32..403
                     /gene="ler"
                     /locus_tag="ACHKIK_005847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFO9086724.1"
                     /translation="MNMETNSHTTSPYIQLIEQIEVLQQEAKRLREQEIQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEDEIIPLKNI"
     gene            418..>529
                     /gene="escE"
                     /locus_tag="ACHKIK_005848"
     CDS             418..>529
                     /gene="escE"
                     /locus_tag="ACHKIK_005848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscE family type III secretion system
                     co-chaperone EscE"
                     /protein_id="HFO9086725.1"
                     /translation="MITITELEDEIIKNKEAANIFIEKINDKKNEIHEKMK"
BASE COUNT          216 a           85 c          109 g          119 t
ORIGIN      
        1 aatatattaa agcatgcgga gattatttat tatgaatatg gaaactaatt cgcacacaac
       61 aagcccatac attcagctta ttgagcaaat tgaagtgtta caacaggaag caaagcgact
      121 gcgagagcag gaaattcaaa gtgtaattga gtcgattcaa aagcagatta cttattacaa
      181 tataacccta caagagctgg gatatactaa tgtgcctgat gatggccttg ctcgccggaa
      241 ctcatcgaaa ggagtttatt atcgcaatga agaagggcag acctggtcgg gagttggccg
      301 acagccacgc tggcttaaag aagcactgtt gaatggaatg aagaaagaag attttcttgt
      361 gaaggacacc gaagacgaaa taataccgct gaaaaatatt taacatgaaa tcattaaatg
      421 ataacgataa ctgaactgga agacgaaata ataaaaaata aagaagccgc aaatattttc
      481 attgaaaaaa taaacgacaa aaagaacgaa atccatgaaa aaatgaaac
//
LOCUS       DAZGJW010000624          528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZGJW010000624 DAZGJW010000000
VERSION     DAZGJW010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.631"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            19..>528
                     /locus_tag="ACHKIK_005849"
     CDS             19..>528
                     /locus_tag="ACHKIK_005849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO9086726.1"
                     /translation="MNKKLMYIFAIFIVAAITCISHPKKTTLRDKAMVNYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQH"
BASE COUNT          171 a          114 c          111 g          132 t
ORIGIN      
        1 aaatcgaagc attcacatat gaataaaaaa ttaatgtata tattcgcaat atttatagtt
       61 gcagcaatta cctgtattag ccaccccaag aaaacaacgt tgcgtgataa agccatggtg
      121 aattatgcct ttgattattt aagctcaccg ggcagtctac cgtttaccac ggcggccacg
      181 gagctttccg cgattcatgg tcactcaacg tcgcaatatc gccttggaga attttatctt
      241 catggtagcg acggtaaacc actggattat acacaggcga gatactggta tgagcaatca
      301 gcagaacagg aaaatccgcg cgcacaaagc aaactggggt ggatctacct caaaggtctg
      361 ggggtcaaac ccgacacccg taaagcaatt ctctggtata aggaagcagc tgaacaaggg
      421 tatgctcatg ctcaatatac tttaggtttg atctacagaa atggctcagg tattaatgtt
      481 aaccattatg aatctcaaaa atggttaaaa ctggccgcca aacaacat
//
LOCUS       DAZGJW010000625          527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZGJW010000625 DAZGJW010000000
VERSION     DAZGJW010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.632"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..>527
                     /locus_tag="ACHKIK_005850"
     CDS             1..>527
                     /locus_tag="ACHKIK_005850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO9086727.1"
                     /translation="MAPVKGAGTTLWVYTGKGDAYANPLSDDDWQRLAKVKDLTPGEM
                     TAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVRAY
                     KIRFPNGTVDVFRGWISSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITPATAIKV
                     TPTSGTVEKGKTTTLT"
BASE COUNT          146 a          117 c          169 g           95 t
ORIGIN      
        1 ctggcgccgg taaaaggtgc cggtaccact ctgtgggttt acaccggcaa gggtgatgct
       61 tatgcaaacc cgttgtcaga tgatgactgg cagcgactgg ctaaggtgaa ggatctgacg
      121 ccgggcgaga tgacggcaga atcctacgat gataactacc tggatgatga agacgcggac
      181 tggagcgcga ccgggcaggg acagaaatct gcaggtgata ccagttttac actggcctgg
      241 aaaccgggag aagaaggtca gaaagggctt ataggctggt ttgaaagcgg ggatgtgcgg
      301 gcctataaaa tccgttttcc gaatggcacg gtggatgtgt ttcgtggctg gatcagcagt
      361 atcggtaagg cagtgacggc gaaagaagtg atcacccgaa cagtgaaagt gaccaacgtg
      421 ggcaaacctt ccgtggcgga agaacgcagc gaaattacgc cggccactgc aattaaggtg
      481 acaccgacat ccggtaccgt ggaaaaagga aaaacaacca ccctgac
//
LOCUS       DAZGJW010000626          526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZGJW010000626 DAZGJW010000000
VERSION     DAZGJW010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.633"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            43..402
                     /locus_tag="ACHKIK_005851"
     CDS             43..402
                     /locus_tag="ACHKIK_005851"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO9086728.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLLS"
BASE COUNT          156 a           85 c          109 g          176 t
ORIGIN      
        1 ttataatgca tattcagaca tataattaaa taagaggtat ttatggctaa tacgaccact
       61 ggtgttacca gaattattaa agcagcgggt tattcatgga aaggcttatg tgcggcatgg
      121 cgctatgaag cagcctttcg tcaggagtgc attctggttc tgtcagcact gataatatcc
      181 ttttggctgg atgttgactc tgttactcga gtattactca ttggttcggc aatgatggta
      241 attattgttg aaataattaa cagtgcaatt gaagcagttg ttgacagaat tggttcagaa
      301 catcacgaat tatcaggcag agcaaaagat atgggttcag ccgcagtgtt gattacgatt
      361 ttagttgcta tcattatatg gggagtttta ttactttcat aacgcccaac atctaaatta
      421 ttcacagtta cctggttaat atttacttaa ggataagagg tattcacctc ttatcctcag
      481 aaaaatgtca gtatctatct gcaggaggat ataatatcat cttctt
//
LOCUS       DAZGJW010000627          523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZGJW010000627 DAZGJW010000000
VERSION     DAZGJW010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.634"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            61..465
                     /locus_tag="ACHKIK_005852"
     CDS             61..465
                     /locus_tag="ACHKIK_005852"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9086729.1"
                     /translation="MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGIND
                     NLLFKWRQYWREGKLRPPSTTENNVPELLPITLDAEDVVPTTSPRSQPVAAATPESLN
                     ISCEVTFRHGSLRLNGAISENILNLLIRELKR"
BASE COUNT          151 a          140 c          117 g          115 t
ORIGIN      
        1 tctggcagat cctgaaattc ctgagagaat agtggacacc aaatatggtg gacgctatcc
       61 atgaaatcat taaccgcagt gcgtaaaaaa agccctaatt atcccgttga gttcaaaatc
      121 aaaatggttg aactctcgca tcgaccagag atctccgtag cgcaactcgc tcgtgagcat
      181 gggatcaacg ataatttgct gttcaagtgg cgccagtact ggcgcgaagg aaaactacgt
      241 cctccttcaa caacagaaaa caacgtgcct gagctgctcc cgataacact tgatgccgaa
      301 gatgttgtcc ctacaacctc cccccggtca caacctgtag ctgctgcgac acctgaatca
      361 ctcaatatca gctgtgaagt gacgttccgg cacggatcac tccgtctgaa tggtgccatc
      421 agcgaaaata tcctgaacct gctgatacgg gagctcaaac gttgatccca ttaccatcag
      481 ggacaaagat ctggctggtc gctggcatca ccgatatgag aaa
//
LOCUS       DAZGJW010000628          520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZGJW010000628 DAZGJW010000000
VERSION     DAZGJW010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.635"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..>520
                     /locus_tag="ACHKIK_005853"
     rRNA            1..>520
                     /locus_tag="ACHKIK_005853"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          154 a          115 c          158 g           93 t
ORIGIN      
        1 gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg aaggacgtgc
       61 taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga tttccgaatg
      121 gggaaaccca gtgtgtttcg acacactatc attaactgaa tccataggtt aatgaggcga
      181 accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga gattccccca
      241 gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtatgtgtg ttagtggaag
      301 cgtctggaaa ggcgcgcgat acagggtgac agccccgtac acaaaaatgc acatgctgtg
      361 agctcgatga gtagggcggg acacgtggta tcctgtctga atatgggggg accatcctcc
      421 aaggctaaat actcctgact gaccgatagt gaaccagtac cgtgagggaa aggcgaaaag
      481 aaccccggcg aggggagtga aaaagaacct gaaaccgtgt
//
LOCUS       DAZGJW010000629          512 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZGJW010000629 DAZGJW010000000
VERSION     DAZGJW010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.636"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..416)
                     /locus_tag="ACHKIK_005854"
     CDS             complement(<1..416)
                     /locus_tag="ACHKIK_005854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO9086730.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELHSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAAS"
BASE COUNT          115 a          157 c          131 g          109 t
ORIGIN      
        1 gaggcagccg aacgggcaaa cagggccgca ttatccgaaa atgcggtgcc tgtccgctgg
       61 ctgatatcca tcagcacttt ctgtgatgac gcaaattcat ccgatgactg cgacgcctgt
      121 ttcagacggg cattcacgga gctccattca tccgccagcg aaatcaggtg tccggtggca
      181 aaggcaccgg caaacgcacc ggtcattccg acagccgaag cgcggatttc cgtcaactgg
      241 ctgtgcagct cagccagagc ccggcgctgc tccctggctg ccgcagcagc ctgacgtccg
      301 ccattctgca gggtccggta atattcactg cccatacggg acgcccgctg gatctccgac
      361 tggaatgact gtgaatttgc cgaaattttg ataatcagtt cacgtaacgt cgccattcac
      421 ctttctccgg gcaaaaaaaa cctgccacag caggttttca tcattattta tgacattgct
      481 gcaaggctca gcgcgtcttc cagcgccgca aa
//
LOCUS       DAZGJW010000630          511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZGJW010000630 DAZGJW010000000
VERSION     DAZGJW010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.637"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>511)
                     /gene="espL2"
                     /locus_tag="ACHKIK_005855"
     CDS             complement(<1..>511)
                     /gene="espL2"
                     /locus_tag="ACHKIK_005855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL2"
                     /protein_id="HFO9086731.1"
                     /translation="EELIHILSARTLDGTIPGLYQALQNGHAQAIKSYGNLVLDTIDK
                     NIDLEYLLSAFKYEAHSSNKYTPGLFSAFQNGHADAIKAYCGVLGNSNLKRGEIIRML
                     EARNYDGAPGLLLAYQNGDINTIQSFFDSLIMLDISKDFIEELLTAKHYDFTGLSLAI
                     SHRHDHVVKL"
BASE COUNT          170 a           99 c           70 g          172 t
ORIGIN      
        1 ataatttgac aacatgatca tgtctatgac tgatcgctaa tgataacccc gtaaaatcat
       61 aatgttttgc agtcaataac tcttcgataa aatcctttga tatatctaac attattagtg
      121 agtcaaaaaa agactgaatc gtattaatat ctccattttg atacgcaaga agtaatcctg
      181 gtgcaccatc ataatttctc gcctcaagca tcctgattat ctcacctctt ttcaaattac
      241 tattgccaag tacgccacaa tatgccttta ttgcgtctgc atgaccattt tgaaatgcag
      301 aaaataatcc aggagtatat ttattagagc tatgtgcctc atatttaaat gctgaaagca
      361 aatactctaa atctatattt ttatcaatag tatccagaac taagttacca taactcttaa
      421 tagcttgagc atgtccattt tgaagggcct gatataaacc aggtatggtt ccatccaaag
      481 ttcttgcaga taatatatgt attaattctt c
//
LOCUS       DAZGJW010000631          494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZGJW010000631 DAZGJW010000000
VERSION     DAZGJW010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.638"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>494
                     /locus_tag="ACHKIK_005856"
     CDS             <1..>494
                     /locus_tag="ACHKIK_005856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086732.1"
                     /translation="KHARSVIDHIDFNCDQSKIAETLFFNLNKEPYKNSPELQELIWE
                     KLVVYVNDFNLSNQEKSRLILRLFDDVKLLFNEVPVSILVNDIFLKDFFMKQPDFAKW
                     YFYQLIKKYEGEQLYLNELGYVYGNEEKTNEIVKKLPGYVIKIFEEKMGNELKIRTRM
                     MKILR"
BASE COUNT          178 a           62 c           95 g          159 t
ORIGIN      
        1 aagcatgccc gctccgtgat cgatcatatt gattttaact gcgatcagtc taaaatagct
       61 gagacattat ttttcaacct caataaagaa ccctataaaa acagccctga gttacaggaa
      121 ttgatttggg agaagttggt tgtatatgtc aatgatttta acttaagcaa tcaagaaaaa
      181 tcacgcttaa tactaaggtt atttgatgat gttaagttgc tatttaatga agtgcctgtc
      241 agcattttag taaatgatat ttttttgaag gattttttca tgaagcaacc tgattttgcg
      301 aagtggtact tctatcaatt aattaagaag tatgagggcg aacagctgta tcttaatgag
      361 ttaggatatg tttatggtaa tgaagaaaaa acaaatgaaa ttgtaaaaaa acttccgggt
      421 tatgtcatta agatttttga agaaaagatg ggtaatgaat taaaaattag aacaagaatg
      481 atgaaaatcc tccg
//
LOCUS       DAZGJW010000632          494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZGJW010000632 DAZGJW010000000
VERSION     DAZGJW010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.639"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            148..471
                     /locus_tag="ACHKIK_005857"
     CDS             148..471
                     /locus_tag="ACHKIK_005857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO9086733.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          110 a          127 c          115 g          142 t
ORIGIN      
        1 ttataaaaat gataatcaaa aaacagcccc cctatttctg acacctacag atggcaagaa
       61 atagcgcctg ccaggcgtct tttccggcca ttgtcgcagc gctgtaacgc gtaaaatagt
      121 gctttctctt actcttctgg ctggaccatg agacttctga ttctgactct ttcattaata
      181 acgctggcgg ggtgtacggt tactcgccag gcccatgtga gcgaagttga cgcggcaaca
      241 ggtattgtac ggttggttta tgaccaggct tttttgcagc atgcccatac cgatcgttat
      301 gtcagtcgcg ggatcgccga tcgcgcatgc cagcaggaag gctataccca cgcggtcccc
      361 tttggtcagc cagtaggcaa ctgtagcctt tttgcgggtt ccttatgttt gaatacggaa
      421 ttcactttgt catatcagtg ccatcattcc gccttccctg tctttctcta aatattttaa
      481 tcagcgaggg gatc
//
LOCUS       DAZGJW010000633          490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZGJW010000633 DAZGJW010000000
VERSION     DAZGJW010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.640"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..490
                     /locus_tag="ACHKIK_005858"
                     /pseudo
     CDS             1..490
                     /locus_tag="ACHKIK_005858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          147 a           87 c          118 g          138 t
ORIGIN      
        1 tcggatattc tactgttgac tatttgtgcc gttatttctg gtgcagaagg ctgggaagat
       61 atagaggatt ttggggaaac acatcccgat gttttgaagt gatatggtga ttttgaaaat
      121 ggtattcctg ttcacgatat cattgccaga gttgtatcct gtatcagtcc tgcaaaattt
      181 cacgagtgct ttattaactg gatgcgtgac tgccattctt cagatgataa agacgtcatt
      241 gcaattgatg gaaaaacgct ccggcactct tatgacaaga gtcgtcgcag gggagcgatt
      301 catgtcatta gtgcgttctc aacaatgcat agtctggtcc tcggacagat caagacggat
      361 gagaaatcca atgagattac agccattcct gaacttctta acatgatgga tattaaagga
      421 aaaatcatca caactgatgc gatgggctgc cagaaagata ttgcagaaaa gatacaaaaa
      481 cagggaggtg
//
LOCUS       DAZGJW010000634          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZGJW010000634 DAZGJW010000000
VERSION     DAZGJW010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.641"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>485
                     /locus_tag="ACHKIK_005859"
     rRNA            <1..>485
                     /locus_tag="ACHKIK_005859"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          121 a          110 c          163 g           91 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaacc
//
LOCUS       DAZGJW010000635          481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZGJW010000635 DAZGJW010000000
VERSION     DAZGJW010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.642"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..346
                     /locus_tag="ACHKIK_005860"
     CDS             <1..346
                     /locus_tag="ACHKIK_005860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFO9086734.1"
                     /translation="FYSELKRHNVSLYIYYLATDNIHIVLENDNIVLVKGLKKVVNVK
                     FSRNKHLIETSYNKLKSKEITFQQYRENLAKAGVFRWVTNIQEHQRYYYAFDNSLLFT
                     ESIQKTTQILPR"
BASE COUNT          185 a           94 c           68 g          134 t
ORIGIN      
        1 attttattct gagctaaaac gtcacaatgt ctcactttat atttactatt tagccacaga
       61 taatattcac attgtgttag aaaacgacaa catagtgtta gtaaaaggac tcaaaaaggt
      121 tgtaaatgtt aaattctcca gaaacaaaca tcttatagag acctcctata ataagttgaa
      181 atcaaaagaa atcacatttc aacaatacag ggaaaatctt gctaaagcag gagttttccg
      241 atgggttaca aatatccagg aacaccaaag atattactat gcctttgata actcattact
      301 atttactgaa agcatccaga aaactacaca gatcttacca cgctaaacca taacgtccgg
      361 cttctctcac tcctgagccg gactgcattg gtttaataaa aaccatcaac aattgtgatt
      421 tagatattcg gaaccattca aatataacaa aaccccgaaa aaacgaggtt tatggataaa
      481 t
//
LOCUS       DAZGJW010000636          479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZGJW010000636 DAZGJW010000000
VERSION     DAZGJW010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.643"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..176)
                     /locus_tag="ACHKIK_005861"
     CDS             complement(<1..176)
                     /locus_tag="ACHKIK_005861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO9086735.1"
                     /translation="MRKLCAVILSAVVWLVAAGTPASAAEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYE"
     gene            complement(244..>479)
                     /locus_tag="ACHKIK_005862"
     CDS             complement(244..>479)
                     /locus_tag="ACHKIK_005862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3672 domain-containing protein"
                     /protein_id="HFO9086736.1"
                     /translation="SSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFS
                     VSAWLVNDWYPTASISDLLVVVMKKATAGISIS"
BASE COUNT          123 a          127 c          107 g          122 t
ORIGIN      
        1 aattcataac ggtatttcac gttaatgccc ttcaggtcat cgctgcctgg catatcagta
       61 tgggtctgaa gatacccggc gcttagtgtg gactgatgct ctgctgcgct cgctggcgta
      121 ccagcggcaa ccagccagac tactgcggac agaataacag cacataattt acgcataatt
      181 acctctcgct tttctgcaat aaaaaaggcg ccatttctgg cgcccgtata tgggttataa
      241 aattcagctg atactgatgc ctgcggtggc tttcttcatc accacaacca gcaaatcgct
      301 gatacttgct gtgggatacc agtcatttac cagccatgct gacaccgaaa actccagcgt
      361 catgtgaccg tgaccggcag gcatatcaat aacgccactg taaatcagcg tattatccag
      421 cgcggtacgg ttataaattt cagcaccgtt tttccgcact atcagacggc atgaggagt
//
LOCUS       DAZGJW010000637          479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZGJW010000637 DAZGJW010000000
VERSION     DAZGJW010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.644"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>479
                     /locus_tag="ACHKIK_005863"
     CDS             <1..>479
                     /locus_tag="ACHKIK_005863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO9086737.1"
                     /translation="LKLRRFISEAILSLIKGEKYSIKHDSLDEKTRNQLNAILSEIEK
                     LTIDNYFKPVETTVVRDTFKIFKRYQKWTENSWNIRGNNNFMLTHKGSKCIDFIQSGQ
                     KKQYLELQRIRDNISYNNFFYTTNDLKSLDNVEIGGIPAKKYLEHGLFSEYRQDGTI"
BASE COUNT          199 a           58 c           82 g          140 t
ORIGIN      
        1 tttaaaacta agaaggttta tatcagaagc tattctgtcc ctcataaaag gggaaaagta
       61 ctcaattaag catgatagct tagatgaaaa aacacgtaat cagttaaatg ctatattgag
      121 tgaaatagaa aagctgacga tagataatta ttttaaacct gttgagacaa cagttgtcag
      181 agatactttt aaaattttta aaagatatca aaaatggaca gaaaacagct ggaatattag
      241 agggaataat aatttcatgc taactcacaa gggaagtaag tgtattgatt ttattcaatc
      301 aggacagaaa aaacaatatt tagaacttca gcgtatcaga gataatataa gttataataa
      361 tttcttttat accacaaatg atctaaaatc tttagataat gtggaaatag gaggaatacc
      421 tgcaaaaaaa tatttagaac acggattgtt ttctgaatat cgacaggacg gtacaattc
//
LOCUS       DAZGJW010000638          476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZGJW010000638 DAZGJW010000000
VERSION     DAZGJW010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.645"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>476
                     /locus_tag="ACHKIK_005864"
     rRNA            <1..>476
                     /locus_tag="ACHKIK_005864"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZGJW010000639          475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZGJW010000639 DAZGJW010000000
VERSION     DAZGJW010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.646"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>475
                     /locus_tag="ACHKIK_005865"
     CDS             <1..>475
                     /locus_tag="ACHKIK_005865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024231387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytotoxin"
                     /protein_id="HFO9086738.1"
                     /translation="IVNGNFNESYFMYTYKKFNERDVFDAISFSVQKRSFSDGDTIIK
                     SNSEAQRDYALTIIQAIVSMIPVFDITLPEVSVPLSMGIIASSMGISFDQLINGDTYE
                     ERRSAIPGVATNAVLLGISFALPYLISKASENKVILSQTVSNEDSILNETNIDNFL"
BASE COUNT          148 a           80 c           99 g          148 t
ORIGIN      
        1 atagtaaatg gaaactttaa tgagtcttac ttcatgtaca cctataaaaa attcaatgag
       61 cgggatgttt ttgatgctat atcattttcc gttcagaaac gtagttttag tgatggcgac
      121 accattatta aatcaaacag tgaagcacaa cgggattatg cactgacaat aatccaggca
      181 atcgtatcaa tgatacctgt gtttgacatt acacttccgg aagtgtctgt tccgcttagc
      241 atgggtatta tagcctccag tatggggatc agttttgatc agctaattaa tggcgatact
      301 tatgaagaac gtcgttcggc aattcctgga gtagcgacaa atgccgtatt gttgggtata
      361 tcctttgcac tcccgtacct gattagtaag gccagtgaaa ataaggtgat acttagtcag
      421 actgtaagta atgaagacag tatattgaat gaaacaaata ttgataattt tcttg
//
LOCUS       DAZGJW010000640          473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZGJW010000640 DAZGJW010000000
VERSION     DAZGJW010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.647"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>473
                     /locus_tag="ACHKIK_005866"
     CDS             <1..>473
                     /locus_tag="ACHKIK_005866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9086739.1"
                     /translation="SDYASQAAQLATEGISGLVNNITEMLNGNKVEWRSWASSVLQEI
                     SKVLMNAAIVNGIKTAANSMSGAGGFLGSIGDWLGGAVANAKGGVYTSANLSAYSNSI
                     VDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVY"
BASE COUNT          109 a          116 c          159 g           89 t
ORIGIN      
        1 tccgattacg cctcacaggc agcacagctg gcaacggagg ggatttcagg actggtgaat
       61 aacatcacgg agatgctgaa cggaaacaaa gtggaatggc gcagctgggc ctcatcagtg
      121 ctgcaggaaa tatcaaaagt tcttatgaat gccgcgattg tcaacgggat caagacggcg
      181 gcaaacagta tgtccggtgc aggaggattt ctcggcagca ttggtgactg gctgggcggt
      241 gcggtggcca atgcaaaagg cggcgtgtat acctcggcaa acctgagcgc gtacagcaac
      301 agcattgtgg acacgcccac gtactttgcg tttgcaaaag gggccgggct gatgggggaa
      361 gccggacctg aagccattat gcccctgacc cgggcggcgg atggctcgct gggcgtacgc
      421 gcggtgggca gtatgaacgg cagtgctggt ctggtgtatt ccccggtcta cca
//
LOCUS       DAZGJW010000641          472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZGJW010000641 DAZGJW010000000
VERSION     DAZGJW010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.648"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>472
                     /locus_tag="ACHKIK_005867"
     CDS             <1..>472
                     /locus_tag="ACHKIK_005867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HFO9086740.1"
                     /translation="CLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILD
                     RTWQHHDVIDIKFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKTLLNGKYFDS
                     GYIPCSRADESIQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQT"
BASE COUNT          149 a           83 c           80 g          160 t
ORIGIN      
        1 atgtctcatt gagcctgagt catctgttcg ttttgctctc agtttccgtt accccggctg
       61 ggcaaaaatg atgattatta atggtgttac ttttactaaa aatgatactc aaaactcact
      121 tattatttta gatagaacat ggcaacatca tgatgtgatt gacattaaat ttatagctga
      181 tatacagttt aatactgact tatgtggtga cacttatatt tccagaggtc ccgttttata
      241 tgcaattgag cttgagagcg atattttaat caaaaaaact ctgctgaacg ggaaatattt
      301 tgactcagga tacataccct gtagtagagc tgatgaatct atccagttct cttatgctga
      361 tatggaatct ttttcatatt caacctcacc agagggcaaa caatacattg aaggctgttt
      421 ttatttaaac aagactaaaa tagtcagaaa acttatacca tttggtcaaa cc
//
LOCUS       DAZGJW010000642          469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZGJW010000642 DAZGJW010000000
VERSION     DAZGJW010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.649"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            246..>469
                     /locus_tag="ACHKIK_005868"
     CDS             246..>469
                     /locus_tag="ACHKIK_005868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086741.1"
                     /translation="MFACTFFFLHGAGCLPVNSVSAPESAIIPYTWLLIAPPHRGIHH
                     AEVFLINSKQKNQALCRLFLFITGHSNTTMP"
BASE COUNT          134 a          123 c           99 g          113 t
ORIGIN      
        1 tgtcattatt cctcccggcc gggatagtgt attaaatcag atatggagtg ggctgtagtc
       61 cggaagcctg aatgacacac ggggactaca gcccaagaaa tgaaaaaagg ccacgcagtt
      121 gcgcagcctg ataaaccctg gttaaaatcc acacgataac aacacaacaa tatcagtatc
      181 tcatgctatt gcccgaaccc attcgggcat tttttaccca taaaaaagcc cctccggaga
      241 ggggcatgtt tgcatgcaca ttctttttct tgcatggtgc cgggtgcctc ccggtgaatt
      301 cagtatcagc acctgaatcc gcgattatcc catatacctg gttgctgatc gcccctccgc
      361 acagggggat tcaccatgca gaagtgtttt taataaacag caaacaaaaa aatcaagcat
      421 tatgcaggct gtttcttttt atcaccggcc acagcaatac cacaatgcc
//
LOCUS       DAZGJW010000643          468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZGJW010000643 DAZGJW010000000
VERSION     DAZGJW010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.650"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..214
                     /locus_tag="ACHKIK_005869"
     CDS             <1..214
                     /locus_tag="ACHKIK_005869"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086742.1"
                     /translation="CADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATIL
                     AGISAGGMYCPECGTVHWPDGVPPPF"
     gene            214..>468
                     /locus_tag="ACHKIK_005870"
     CDS             214..>468
                     /locus_tag="ACHKIK_005870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO9086743.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCSHIKKVCFRCKSRSCPHCGVKAGAQWI"
BASE COUNT           97 a          124 c          139 g          108 t
ORIGIN      
        1 ctgcgcagac ctgaatattc ccttctttgt ggatgccgga aacaaaaagc tggtcttctg
       61 gtttgtacgt gttgatgacg aagggtatcc tgaaatagcc cgctgcacgg agcgggagtt
      121 tgcgaccatt cttgccggta tcagcgccgg cggcatgtac tgcccggagt gtggcacggt
      181 tcactggccg gacggagtcc ccccgccctt ctgatgcttc cccgttttgc cgacattttt
      241 cagcagggaa accgctggct taactggctg gagaaacaac cggaaggttc agtgcgtccg
      301 gtagtcattg agtctgtgac aaaaatcatg gcctgcggga ccacgctgat ggggtacaca
      361 cagtggtgct gttcatctcc ggactgcagc cacataaaaa aggtctgctt ccggtgtaaa
      421 agtcgctcct gcccgcactg cggagtgaag gctggcgcac agtggata
//
LOCUS       DAZGJW010000644          467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZGJW010000644 DAZGJW010000000
VERSION     DAZGJW010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.651"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            106..>467
                     /locus_tag="ACHKIK_005871"
     CDS             106..>467
                     /locus_tag="ACHKIK_005871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFO9086744.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVL"
BASE COUNT          133 a          117 c          105 g          112 t
ORIGIN      
        1 caaccaaatt ctccccacat caacctcatt gtgtgggtat aattgcgggc ataccccagt
       61 tcgacagaat ttgtaccctc tttaacgcat tcagaaggct gaactatggc actgactgac
      121 gcaaaaatcc gggctgcaaa gcccactgac aaggcttata aactcactga cggggctggc
      181 atgttcctgc tggtacatcc taatggttcc cgttactggc gtctccgtta tcgtattctg
      241 ggtaaagaga agactctggc acttggtgtg tatccagaag tatctctctc cgaagctcgt
      301 acaaaacggg atgaggcccg aaaactgatt tcggaggggg ttgacccttg cgaacagaaa
      361 agagctaaaa aagtagtccc tgatttacaa ctctctttcg aacatattgc acgacgctgg
      421 catgccagta ataaacaatg ggcacaatca cacagcgata aagtact
//
LOCUS       DAZGJW010000645          465 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZGJW010000645 DAZGJW010000000
VERSION     DAZGJW010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.652"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            25..>465
                     /locus_tag="ACHKIK_005872"
     CDS             25..>465
                     /locus_tag="ACHKIK_005872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9086745.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHI"
BASE COUNT          155 a           83 c          105 g          122 t
ORIGIN      
        1 taaattttcg aggagatcat tccagtggga cgtaaatggg ccaatattgt tgctaaaaaa
       61 acggctaaag acggtgcaac gtctaaaatt tatgcaaaat tcggtgtaga aatctatgct
      121 gctgccaaac aaggtgaacc cgatccagaa ttaaacacat ctttaaaatt cgttattgaa
      181 cgtgcaaagc aggcacaagt tccaaagcac gttattgata aagcaattga taaagccaaa
      241 ggcggcggag atgaaacgtt cgtacaggga cgttatgaag gctttggtcc taatggctca
      301 atgattattg ctgagacatt gacttcaaat gttaaccgta cgattgctaa cgttcgcaca
      361 attttcaata aaaaaggcgg caatatcgga gcggcaggtt ctgtcagcta tatgtttgac
      421 aatacgggtg tgattgtatt taaaggaaca gaccctgacc atatt
//
LOCUS       DAZGJW010000646          465 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZGJW010000646 DAZGJW010000000
VERSION     DAZGJW010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.653"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..450
                     /locus_tag="ACHKIK_005873"
     CDS             <1..450
                     /locus_tag="ACHKIK_005873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086746.1"
                     /translation="YWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGVSFI
                     SMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVRGKE
                     TETISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
BASE COUNT          133 a           90 c           87 g          155 t
ORIGIN      
        1 tactggacaa gaaaacgtat tcctgaactt tcagcacttc caccttcatt aagaaagaaa
       61 aattttactg acgcatataa tgcagcatct tctcatatcg aatactggat aggtgctggc
      121 gtctcattta tctcgatgat gattttatcc agagtatatg actttctttt acccgcccaa
      181 gatactttcc cgggtgatat catcagaagt ttatgtgttg tgtgtccatc catattaatc
      241 tggtttcagt tttcggtata tgtcatgagg aaatattaca gacatattct ggtgcgagga
      301 aaagaaaccg aaaccatttc agaaaggttg atacgtgagg ctgatacacg agaatatgaa
      361 ctatggcgtc cggtccgtcg ctttttctcc attgttttcc tcattgttct attaggttgc
      421 attcattcac tgataacaac tataaaataa aagttatggc tgttc
//
LOCUS       DAZGJW010000647          461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZGJW010000647 DAZGJW010000000
VERSION     DAZGJW010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.654"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..141)
                     /locus_tag="ACHKIK_005874"
     CDS             complement(<1..141)
                     /locus_tag="ACHKIK_005874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086747.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVI"
     gene            159..350
                     /gene="yfgG"
                     /locus_tag="ACHKIK_005875"
     CDS             159..350
                     /gene="yfgG"
                     /locus_tag="ACHKIK_005875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO9086748.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          125 a          115 c           78 g          143 t
ORIGIN      
        1 tattacatca tcattgtaaa taattaaatt aacttccata acattaaaat atgtatccac
       61 tgacgctttt ttacataacg aagaattgac cattttgtcc tgttgtgcct taatgtaagt
      121 accgtccaca gcgtgggaca tacttcaagg aaccttttgt gagtcaggca accagtatgc
      181 gaaaacgaca ccgatttaac agtcgcatga cccgtatcgt actgctcatc agctttatct
      241 tcttctttgg ccgttttatc tactcgtccg tcggtgcctg gcagcaccat cagagcaaaa
      301 aagaagctca gcaatccaca ctctccgtcg aatcaccggt acaacgttag cggttacctt
      361 ctccactttc acagaaccta acggcacttc gctgtcggat gcttttgctc tttggaatta
      421 tcaaagcggc agatattctt tcatcttaaa ttttacgtct t
//
LOCUS       DAZGJW010000648          457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZGJW010000648 DAZGJW010000000
VERSION     DAZGJW010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.655"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            92..277
                     /locus_tag="ACHKIK_005876"
     CDS             92..277
                     /locus_tag="ACHKIK_005876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO9086749.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLSTERSGMNYIIK"
BASE COUNT          153 a          105 c           91 g          108 t
ORIGIN      
        1 cgggtattca gtgttttgat tctgctctgg agtgtacgaa gctcagccat gtaaaatccc
       61 cgtcatatgg caatcagtaa aggaaataaa tatgtcatcg aaaaaccgga cccgcagaac
      121 cacaacccgc aatatccgtt tccccaatca catgattgaa cagatcaata tcgcccttga
      181 acataaaggt tccggtaact tttcagcgtg ggttattgaa gcctgcagaa gaaggctgtc
      241 aacagagcgt tcgggtatga attacataat taagtaacat ggtgttcaca gaacacacag
      301 ttaccggaca catcagtttt ccattcgtcc cccggcagta caggcttccc gtctgacggg
      361 atagcctgaa aaaacacaga aaattatttg ttataattaa tataacttac tcaaaaaaaa
      421 gcgacgagaa aatcagcatc aacgagcaat aagcgcc
//
LOCUS       DAZGJW010000649          453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZGJW010000649 DAZGJW010000000
VERSION     DAZGJW010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.656"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>453
                     /locus_tag="ACHKIK_005877"
     CDS             <1..>453
                     /locus_tag="ACHKIK_005877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO9086750.1"
                     /translation="VRYTAAVPSRVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKI
                     IIMGRPDEEGTLLRVDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVF
                     RVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADP"
BASE COUNT          108 a          103 c          143 g           99 t
ORIGIN      
        1 gtgcgttaca cggctgcggt gccttcccgg gtggtttatc tgacggcggg cattgactcg
       61 cagcgaaacc gttttgagat gtatgtctgg ggatgggcac cgggagagga agcctttctg
      121 gtggataaaa tcatcattat ggggcgtccc gatgaggaag ggacgctgtt acgtgtggat
      181 gcggcgatca acaaaaaata ccgccatgcg gatggcaccg aaatgaccat ttcccgtgtc
      241 tgctgggaca ccggggggat cgatggtgaa attgtttatc agagatcaaa aaaacacggt
      301 gttttccggg tgctgccggt aaaaggcgcg tctgtctatg gcaagccggt gatcaccatg
      361 ccgaaaaccc gcaatcagcg gggcgtgtat ctgtgtgaag tgggaacgga caccgcaaaa
      421 gaaattctct atgcccgtat gaaagccgat ccc
//
LOCUS       DAZGJW010000650          452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZGJW010000650 DAZGJW010000000
VERSION     DAZGJW010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.657"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>452
                     /locus_tag="ACHKIK_005878"
     CDS             <1..>452
                     /locus_tag="ACHKIK_005878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO9086751.1"
                     /translation="VRYTAAVPARVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKI
                     IIMGRPDEEETLLRVDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVF
                     RVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADP"
BASE COUNT          110 a          100 c          142 g          100 t
ORIGIN      
        1 gtgcgttaca cggcggcggt gcctgcccgg gtggtttatc tgacggcggg cattgactcg
       61 cagcgaaacc gttttgagat gtatgtctgg ggatgggctc cgggagagga agcctttctg
      121 gtggataaaa tcatcattat ggggcgtcct gatgaggaag agacgctgtt acgtgtggat
      181 gcggcgatca acaaaaaata ccgccatgcg gatggcaccg aaatgactat ttcccgtgtc
      241 tgctgggaca ccggggggat cgatggtgaa attgtttatc agagatcaaa aaaacacggt
      301 gttttccggg tgctgccggt aaaaggcgca tctgtctatg gcaagccggt gatcaccatg
      361 ccaaaaaccc gcaatcagcg gggcgtgtat ctgtgtgaag tgggaacgga caccgcaaaa
      421 gaaattctct atgcccgtat gaaagccgat cc
//
LOCUS       DAZGJW010000651          451 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZGJW010000651 DAZGJW010000000
VERSION     DAZGJW010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.658"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            63..>451
                     /gene="tnpA"
                     /locus_tag="ACHKIK_005879"
     CDS             63..>451
                     /gene="tnpA"
                     /locus_tag="ACHKIK_005879"
                     /inference="COORDINATES: protein motif:HMM:NF038385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO9086752.1"
                     /translation="MKHRTWITEALRLHFEEHLPRVVAGRRLGVPKSTVCSMFVRFRR
                     AGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPRRPNFPYEFKI
                     ALVEQSLQPGACVAQIARENGINDNLL"
BASE COUNT          102 a          123 c          110 g          116 t
ORIGIN      
        1 tctggtctgt ttcctccggc tctacaaaaa taatgtccat catttttaat ggacactatc
       61 gtatgaaaca ccggacctgg atcactgaag ctttacgtct tcactttgaa gaacatttac
      121 cccgggttgt ggccgggcgt cgcctgggtg taccaaaatc aacagtttgt agtatgttcg
      181 tgcgctttcg gagagctggc ctttcgtggc ctttgcccgc aggcatgtcg gagcaggaac
      241 ttgatgcctg cctttacgga caattttcca cggtaccagt cgtacgtcct gaaagcaccg
      301 ttatatccga agcccccgtg gtaaaaaaac gtccccggcg gcccaacttc ccttatgagt
      361 ttaaaatcgc cttagtggag cagtcactgc agcccggagc ctgtgtggcg cagatcgccc
      421 gggaaaacgg aatcaacgat aacctgctct t
//
LOCUS       DAZGJW010000652          446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.659, whole genome shotgun sequence.
ACCESSION   DAZGJW010000652 DAZGJW010000000
VERSION     DAZGJW010000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.659"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..383
                     /locus_tag="ACHKIK_005880"
     CDS             <1..383
                     /locus_tag="ACHKIK_005880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO9086753.1"
                     /translation="LVEDHRGKTVYDVASGDALFISELGPLPENVTWLSPEGEFQKWN
                     GTAWVKDAEAEKLFRIRKAEETKNSLMQMASEHIAPLQDAVDLDIATEEEASLLTAWK
                     TYRVLLNRVDTTVASDVEWPVAPQ"
BASE COUNT          133 a           91 c          120 g          102 t
ORIGIN      
        1 atctcgttga agaccatcgg ggaaaaaccg tctatgacgt ggcttccggc gacgcgttat
       61 ttatttctga acttggccca ttaccggaaa atgtcacctg gttatccccg gaaggggagt
      121 ttcagaagtg gaacggcaca gcctgggtga aagatgcaga agcagaaaaa ctgttccgga
      181 tccggaaggc ggaagaaaca aaaaacagcc tgatgcagat ggccagtgag catattgcgc
      241 cacttcagga tgccgtagat ttggatattg cgacggagga agaggcatcg ttactgactg
      301 catggaagac atatcgggta ttgttgaatc gtgttgatac aacagtagca tcggatgttg
      361 agtggccagt cgccccgcaa taaaaggata aagccatcga tagaaatatt gatggcttca
      421 tgtactctat ttatacaata caacac
//
LOCUS       DAZGJW010000653          446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.660, whole genome shotgun sequence.
ACCESSION   DAZGJW010000653 DAZGJW010000000
VERSION     DAZGJW010000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.660"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>446
                     /locus_tag="ACHKIK_005881"
     CDS             <1..>446
                     /locus_tag="ACHKIK_005881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086754.1"
                     /translation="EVQQPLHSIISRQKEHVKNLLSGLLNEFSNKLRQQGLSLKTNVL
                     SVNNFKESKINSDTVEVTVTDLQGRLYSVDIDTRVIGLTFKEGINSLSEALEHMNIDA
                     IMSVIGLVQYTRMIKTNDNISAIDHAGAVSDIKNIVDKFLGGILTFT"
BASE COUNT          168 a           62 c           78 g          138 t
ORIGIN      
        1 gaagttcaac aaccccttca ttctattatt tcacggcaga aagaacatgt aaaaaactta
       61 ctatctggat tacttaatga gttttcaaat aagttaagac agcaaggcct ttcacttaaa
      121 acaaatgttc tttcagttaa taacttcaaa gaatcaaaga taaatagtga tactgtggag
      181 gttacagtaa ctgatctgca aggaagactt tatagtgttg atatagatac aagagttatt
      241 ggcttgactt ttaaagaggg gataaatagt ttatctgaag cactcgaaca catgaatata
      301 gatgcaatta tgtctgttat tggcttggtt caatatacac gaatgataaa aacaaatgac
      361 aatattagcg caatagatca tgcaggagca gtatcagata ttaaaaatat tgtagacaaa
      421 tttttaggcg gaattttaac ttttac
//
LOCUS       DAZGJW010000654          445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.661, whole genome shotgun sequence.
ACCESSION   DAZGJW010000654 DAZGJW010000000
VERSION     DAZGJW010000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.661"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..231
                     /locus_tag="ACHKIK_005882"
     CDS             <1..231
                     /locus_tag="ACHKIK_005882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086755.1"
                     /translation="ISADIILTRTPHITEFSSDSITAQIKKNGINIQTGRMVSQKHLI
                     ITHDQEGKHLTTWYQQISLRVHSYKKHTEEVR"
BASE COUNT          169 a          103 c           66 g          107 t
ORIGIN      
        1 atctcagcag atattatcct gaccaggaca cctcacatta ctgaattctc ttccgacagt
       61 atcactgcac aaataaaaaa gaacgggata aatattcaga ccggaagaat ggtgtcacaa
      121 aagcatttaa tcattacgca tgaccaggaa gggaaacact taaccacatg gtatcaacag
      181 atatcattac gtgtgcactc ttataaaaaa cacacagaag aagtaagata attataaacg
      241 taaatttaat atctcccact gacatctccc ccacaataca ctaaaaaata ctcacagcag
      301 taaacacaga cacacctgat aatcatacag ccgataaata ttttcccctt tacggagaaa
      361 tcagcctgct gtttaacaat aacggcgtat tttcatggaa ggagatcacc cagtaacgga
      421 taagtcctga tctccttcat gttaa
//
LOCUS       DAZGJW010000655          439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.662, whole genome shotgun sequence.
ACCESSION   DAZGJW010000655 DAZGJW010000000
VERSION     DAZGJW010000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.662"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..321
                     /locus_tag="ACHKIK_005883"
     CDS             <1..321
                     /locus_tag="ACHKIK_005883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO9086756.1"
                     /translation="TRIKNLLCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPS
                     RFEILIPLVQTLVRDNVKLNNDVYKELNKFMHDYDKTSSEMRKYLKSINECMFLMKNI
                     AHQN"
BASE COUNT          175 a           77 c           68 g          119 t
ORIGIN      
        1 accagaataa aaaatttgct ctgtgaagat attatgcctg gtcgatggac cactcaactt
       61 gtcaataagc acttaggtta cagatacaca ggagtattca aaaccctggc aagtattgat
      121 gacaaaccgt cccgtttcga aattttgata ccattggtac aaactttagt tcgcgacaat
      181 gtaaagctaa ataatgatgt gtataaagaa cttaataagt ttatgcacga ttacgacaaa
      241 acatcatcag aaatgagaaa ataccttaaa agcatcaacg agtgtatgtt ccttatgaaa
      301 aacatagctc atcaaaatta aaaaatctca aaaaagaagt aatttcagaa tgaaattaac
      361 tgtgatgatt attaattcac tacagaggaa taaaaaacaa tctgaagcat gctctgaaac
      421 atcctactct gtcactcta
//
LOCUS       DAZGJW010000656          438 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.663, whole genome shotgun sequence.
ACCESSION   DAZGJW010000656 DAZGJW010000000
VERSION     DAZGJW010000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.663"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>438
                     /locus_tag="ACHKIK_005884"
     CDS             <1..>438
                     /locus_tag="ACHKIK_005884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9086757.1"
                     /translation="RLGYAADDLGFVGDPYQEARQRYISNALEAWRNNEANKPKSRGG
                     KSETEKAEDSFSRLLKQQKEQLALVGQNTELAKLKYQTAQGELKTLTEMQKQELLRNA
                     ALIDQQKIREQLRSREETLKNENAAARASNDAELLGYGQGERA"
BASE COUNT          124 a           96 c          150 g           68 t
ORIGIN      
        1 ccggctgggg tatgcggcgg atgacctcgg ttttgtgggt gatccgtatc aggaggcgag
       61 acagcgttat atcagtaatg ccctggaagc ctggcgcaat aacgaggcga ataaacccaa
      121 atcccggggt ggaaaatcag agacggaaaa agcggaagac agtttttccc ggctgctgaa
      181 gcagcagaaa gagcaactgg cactggtggg gcagaataca gagctggcga agctgaaata
      241 ccagactgcg cagggcgaac tgaaaaccct gacggagatg cagaagcagg agctgctgcg
      301 taatgcggcc ctgattgacc agcaaaaaat ccgggaacag ttgcgatccc gggaagagac
      361 actgaagaat gagaatgcgg ctgcgcgtgc gtcgaatgat gctgaactgc tggggtacgg
      421 gcagggggaa cgagccag
//
LOCUS       DAZGJW010000657          436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.664, whole genome shotgun sequence.
ACCESSION   DAZGJW010000657 DAZGJW010000000
VERSION     DAZGJW010000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.664"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            10..159
                     /locus_tag="ACHKIK_005885"
                     /pseudo
     CDS             10..159
                     /locus_tag="ACHKIK_005885"
                     /inference="COORDINATES: protein motif:HMM:NF025712.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
     gene            169..312
                     /locus_tag="ACHKIK_005886"
     CDS             169..312
                     /locus_tag="ACHKIK_005886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086758.1"
                     /translation="MKVWVYDPETILNVEERRRTEVYIHNLNEDGFAPVLVKATPQEV
                     NRG"
     gene            305..>436
                     /locus_tag="ACHKIK_005887"
     CDS             305..>436
                     /locus_tag="ACHKIK_005887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO9086759.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     "
BASE COUNT          136 a           80 c          107 g          113 t
ORIGIN      
        1 ggatgattta tgaatctata tcgctgccca ttctgtggtt ccactgtgct taacatcgga
       61 tactcattca gtatcagagg gaaaatgcgc tatgtgtcat gcaaatgtgg tgcccaagga
      121 ccagaaaaac gaactagatc tgaagcgatt tcttcatgga atagtcgaat gaaagtatgg
      181 gtttatgatc cagaaacaat attaaacgtt gaagagcgca ggagaacaga agtctatatt
      241 cataacctta atgaagatgg atttgcacca gtgcttgtaa aggctactcc gcaggaggtt
      301 aaccgtggct aacctacaac ttgccgtcaa aggtgaatac ttcgatgcca tgattcgagg
      361 agagaaaacg gaagagtatc gcctgtgtaa tgactattgg aataagcgaa ttatgttccg
      421 ggagtatgac cgcctg
//
LOCUS       DAZGJW010000658          433 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.665, whole genome shotgun sequence.
ACCESSION   DAZGJW010000658 DAZGJW010000000
VERSION     DAZGJW010000658.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.665"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>433
                     /locus_tag="ACHKIK_005888"
     CDS             <1..>433
                     /locus_tag="ACHKIK_005888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO9086760.1"
                     /translation="LKDLERAYKNFFRKRAAFPRFKKRGQNDAFRYPQGVKLDQENSR
                     IFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPSASMIGLDA
                     GVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQLSRKVKFS"
BASE COUNT          118 a          107 c          116 g           92 t
ORIGIN      
        1 cactgaaaga ccttgagcgg gcttacaaaa acttcttccg gaagcgggct gcttttcccc
       61 gattcaaaaa gcggggacag aatgatgcat tccgctaccc gcagggcgta aagctcgatc
      121 aggaaaacag ccgtattttt ctgccgaaac tgggctggat gcgctaccgg aatagccgtc
      181 aggtcacggg tgttgtgaaa aatgtcacgg tcagccagtc ctgcggtaag tggtacatca
      241 gtattcagac agaaagtgaa gtatcaactc cggttcaccc ttcagcatca atgatcgggc
      301 tggatgctgg cgtggctaaa ctcgccacgc tgtcagatgg cacagtcttt gggcctgtaa
      361 acagttttca gaaaaaccag aagacgctgg cgagacttca gcgacagtta agccgcaagg
      421 tcaaattcag caa
//
LOCUS       DAZGJW010000659          426 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.666, whole genome shotgun sequence.
ACCESSION   DAZGJW010000659 DAZGJW010000000
VERSION     DAZGJW010000659.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.666"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>426
                     /locus_tag="ACHKIK_005889"
     CDS             <1..>426
                     /locus_tag="ACHKIK_005889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcdB toxin N-terminal helical domain-containing
                     protein"
                     /protein_id="HFO9086761.1"
                     /translation="SFLCNLARLLYSMFNGSVYFPSRDDENSSSPSPTLPTIHQSERK
                     ELLQQKSINHLKRSDTIDEYIKIRKKRGIEDQTTTTSQSWIINELLNGVDRNTIPFQK
                     ISELNDIIQSYENMQTKNSRKGIEILVKQGELLSSLINDD"
BASE COUNT          173 a           68 c           66 g          119 t
ORIGIN      
        1 tcttttttat gtaatcttgc cagactgcta tatagcatgt ttaacggtag tgtctatttt
       61 ccttcccgtg atgatgaaaa tagttcatcc cccagtccaa ctctacctac tatacatcag
      121 tctgaaagga aggagttatt acaacaaaaa agcatcaatc atttaaaaag aagtgatacc
      181 atcgatgaat acattaaaat aagaaaaaaa agaggcattg aagatcaaac aacaactacc
      241 tctcaaagtt ggataattaa tgaactgtta aatggagttg atagaaatac cattccattt
      301 cagaaaataa gtgagctcaa tgacatcata caatcatatg aaaatatgca aactaaaaat
      361 agccgaaaag gtatagaaat attagttaag cagggagaat tgttatcatc attaataaat
      421 gatgac
//
LOCUS       DAZGJW010000660          425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.667, whole genome shotgun sequence.
ACCESSION   DAZGJW010000660 DAZGJW010000000
VERSION     DAZGJW010000660.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.667"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>425
                     /locus_tag="ACHKIK_005890"
     CDS             <1..>425
                     /locus_tag="ACHKIK_005890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFO9086762.1"
                     /translation="NKINKFNNYLFRWQGLDGSEILMHNFPEDTYDSRARARSLEYIE
                     QNYNEKEICPYAFMVYGVGDGGAGPGEEHIERLTRIRNIDGLPHVDFSRVDKFFTHAD
                     AFRESLPIISGELYFEAHQGCFTSESATKAHNRIMENKL"
BASE COUNT          123 a           93 c           89 g          120 t
ORIGIN      
        1 aacaagatta ataaatttaa taattacctt ttccgctggc aaggcctgga tggctcagaa
       61 attctgatgc ataactttcc tgaagatacg tatgatagcc gagcccgcgc tcgctcttta
      121 gaatatattg aacaaaacta taatgaaaaa gaaatctgtc cctatgcgtt tatggtgtat
      181 ggtgtcggtg atggcggcgc tggccccggt gaggaacata tagaacgatt aacacgcata
      241 cgcaatattg atggcttacc tcatgttgat ttcagccgtg ttgataagtt cttcactcac
      301 gccgacgcct ttcgcgaatc cttgcccatt atttcgggtg aactttactt cgaggctcat
      361 caagggtgct ttacatcaga gtcagcaact aaagcccata atcgaattat ggaaaataaa
      421 ctcca
//
LOCUS       DAZGJW010000661          425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.668, whole genome shotgun sequence.
ACCESSION   DAZGJW010000661 DAZGJW010000000
VERSION     DAZGJW010000661.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.668"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            68..286
                     /locus_tag="ACHKIK_005891"
     CDS             68..286
                     /locus_tag="ACHKIK_005891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086763.1"
                     /translation="MFKYAEMLMAEEDDKVCELIFKQQDNLILKINVRFPRIIIKKYR
                     TLQGKVVWLWILPFVIEWLIVCTLGVNS"
BASE COUNT          130 a           53 c           92 g          150 t
ORIGIN      
        1 tccataagca ttattgcctg ggaactggac atataattac tcttctgtgc taaaggaacg
       61 ttctgaaatg tttaagtatg cagagatgtt gatggcggaa gaagatgata aagtatgtga
      121 acttattttt aaacaacaag ataatttgat tttaaaaata aatgttagat ttcccagaat
      181 aattataaag aaataccgaa cattacaagg taaagtcgta tggctatgga tattgccatt
      241 tgttattgaa tggttaattg tgtgtacttt gggtgtaaac agttagcttg gtaaaagtaa
      301 atatcctgat tgttgtttgt taaggagtga gatgcctgtg cgtctggtgt ctattatatt
      361 cccaatatta ttggcgctat tcttgctggc gtaggcatcg ccatactatg aatattgtgt
      421 ttgtc
//
LOCUS       DAZGJW010000662          422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.669, whole genome shotgun sequence.
ACCESSION   DAZGJW010000662 DAZGJW010000000
VERSION     DAZGJW010000662.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.669"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(2..292)
                     /locus_tag="ACHKIK_005892"
     CDS             complement(2..292)
                     /locus_tag="ACHKIK_005892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO9086764.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATKEEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            complement(289..>422)
                     /locus_tag="ACHKIK_005893"
     CDS             complement(289..>422)
                     /locus_tag="ACHKIK_005893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HFO9086765.1"
                     /translation="RGEALPEPVKQLPVMGGRPLNRAQALAKIAELKAKFGLKGASV"
BASE COUNT           85 a          126 c           90 g          121 t
ORIGIN      
        1 gtcatctttt aactccatat accgccaata cccgtttcat cgcggcactc tggcgacact
       61 ccttaaaaat taggttcgtg ctcatctttc cttcccgttc ttccttggta gcaaaccggt
      121 aatacaccgt tcgccagacc ttaccttcga taaccagaag acctgcccgt gccattttag
      181 ccgcggcctg atttatgctg gttactgttg cgcctgttag cgcggcaacg tccggcgcac
      241 agaagctatt atgcgtcccc aggtaatgaa taattgcctc tttgcccgtc atacacttgc
      301 tcctttcagt ccgaacttag ctttgagttc tgcgatcttc gccagagcct gtgcacgatt
      361 tagaggtcta ccgcccatga caggaagttg ttttactggt tcagggagcg cctcaccacg
      421 gt
//
LOCUS       DAZGJW010000663          420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.670, whole genome shotgun sequence.
ACCESSION   DAZGJW010000663 DAZGJW010000000
VERSION     DAZGJW010000663.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.670"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            143..>420
                     /locus_tag="ACHKIK_005894"
     CDS             143..>420
                     /locus_tag="ACHKIK_005894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HFO9086766.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALRE"
BASE COUNT          104 a          100 c          117 g           99 t
ORIGIN      
        1 ggcctttttt gtatctgcgc cacgcccggc gcatatcaac cacagagcct ttcgggggtg
       61 agcttacgga gtggtcagtg tgactttctc tgtgggcaga tcgctcccgg gcgttggctc
      121 acccacccaa aggaacgtca cgatgtttgg aatcttcaaa aagaaaaccc gcagagcggc
      181 agcggaaatt aaaaagtttg agaaacgcga tctggcacag gcggtgatta acgctgcata
      241 cctggtggcc tatgcagatg gtgaatgcga ggcatccgag aaagcgaaga tcgaacaggt
      301 cttacgtaat cagcctgcgt tgtctgcgtt tacctcggaa attaatgcga ttagcgcaac
      361 tattatcggt cagctggata ccaattttaa aattggtcgt cgtgccgcgt tacgcgagat
//
LOCUS       DAZGJW010000664          414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.671, whole genome shotgun sequence.
ACCESSION   DAZGJW010000664 DAZGJW010000000
VERSION     DAZGJW010000664.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.671"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(59..355)
                     /locus_tag="ACHKIK_005895"
                     /pseudo
     CDS             complement(59..355)
                     /locus_tag="ACHKIK_005895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT           97 a          110 c           86 g          121 t
ORIGIN      
        1 tggacaccac tttgtctaat tcgtcagatt ctgaccagac ggttcaggct gtacgcttac
       61 gattacgctg aacttcacgt gagatcgtcg aaggactgcg attcagcgca gtagctatcg
      121 cacgaatgct cattttggct gacaaaccag ctcgtatctc ctcgcgctca gacagtgtca
      181 ggtgagctac agcccgctta cgctcatggg gttttatgcc gccagtatcc cttaacatag
      241 tgaagatcgt tccgggtttt gaacccagga tattcgctat ttcactgaag cctgttccgt
      301 tcttccatag ttcaaaaaca gaggcttttt cctctgctgt aaatgttcgt ctcattcaaa
      361 aaacctccgc aaccccatgt tttcacataa ctgttgcgtt gaccaattga atct
//
LOCUS       DAZGJW010000665          413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.672, whole genome shotgun sequence.
ACCESSION   DAZGJW010000665 DAZGJW010000000
VERSION     DAZGJW010000665.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.672"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            85..>413
                     /locus_tag="ACHKIK_005896"
     CDS             85..>413
                     /locus_tag="ACHKIK_005896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9086767.1"
                     /translation="MSSSLPDDINALKRLLAEQEALNRALLEKLNEREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLKALQKESDTLTGRVDDPAVQRPLRQTRTRKPF
                     PESLPRD"
BASE COUNT          103 a          127 c          104 g           79 t
ORIGIN      
        1 cctaaaagac tgcttacgtc cctgactatg ttgtaagcct ctttatcctg gtcgacgctg
       61 aatgagcctg gtaatatacc cggtatgagc agctcacttc ctgacgatat caatgcactg
      121 aaacgtctcc ttgccgaaca ggaggcgctg aaccgtgccc tgctggaaaa gctgaacgag
      181 cgtgaacgcg aaatagacca tctgcaggca cagctggata agctgcgccg gatgaacttc
      241 ggcagccgct ccgaaaaagt ctcccgtcgt atcgcacaga tggaagctga cctgaaggca
      301 cttcagaaag aaagtgatac ccttaccggt cgggttgacg acccggccgt gcagcgcccg
      361 ctgcgtcaaa cccgcacccg caaaccgttc cccgaatcac tcccccgcga tga
//
LOCUS       DAZGJW010000666          413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.673, whole genome shotgun sequence.
ACCESSION   DAZGJW010000666 DAZGJW010000000
VERSION     DAZGJW010000666.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.673"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>413
                     /locus_tag="ACHKIK_005897"
     CDS             <1..>413
                     /locus_tag="ACHKIK_005897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086768.1"
                     /translation="KSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTAQGELK
                     TLTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERAR
                     ERMRELQQIRDSFRQKDADLQSQYQTGDISEDFYR"
BASE COUNT          125 a           94 c          132 g           62 t
ORIGIN      
        1 gaaaatcaga gacggaaaaa gcggaagaca gtttttcccg gctgctgaag cagcagaaag
       61 agcaactggc actggcgggt cagaatacag agctggcgaa gctgaagtac cagactgcgc
      121 agggcgaact gaaaaccctg acggagatgc agaagcagga actgctgcgt aacgcggccc
      181 tgattgacca gcaaaaaatc cgggaacagt tgcgatcccg ggaagagacc ctgaagaatg
      241 ataatgtggc tgcgcgtgca tcaaatgaag ccgaactgct gggatacggg cagggagaac
      301 gagccaggga acgcatgcgg gagttgcagc agatccgcga cagcttccgc cagaaggatg
      361 cggaccttca gtctcagtat cagaccgggg atatcagtga ggatttttac aga
//
LOCUS       DAZGJW010000667          412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.674, whole genome shotgun sequence.
ACCESSION   DAZGJW010000667 DAZGJW010000000
VERSION     DAZGJW010000667.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.674"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>412)
                     /locus_tag="ACHKIK_005898"
     CDS             complement(<1..>412)
                     /locus_tag="ACHKIK_005898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9086769.1"
                     /translation="WRGFTRSVLSMLTEIFLKQAMVGIVGSIGSAMGGAFGGGASAST
                     GTAIQAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGY
                     AEGGYVGGAGSPAQMRRAEGISFNQNNHVVIQNDG"
BASE COUNT           93 a          148 c           97 g           74 t
ORIGIN      
        1 gccgtcgttc tgaatcacca cgtgattgtt ctgattaaaa ctaatgcctt ccgcccgccg
       61 catctgcgcc ggacttccgg caccgcccac ataaccacct tccgcatacc cgcgcatcag
      121 acgatacagg ttgccgacgc caatccggct ggttgcctcc ttcgtgaaga caaactcccc
      181 gcggtggaca ataccggcag gttcgtattt gccaccggtt cccgtaaatc ctccggtcgc
      241 gaaatggaag ttcgccgccg cagcctgaat ggccgtcccc gtggaggcag acgccccacc
      301 accgaaagca ccacccatgg cgctgccaat actcccgaca atccccacca tggcctgctt
      361 cagaaaaatc tctgtcagca tggagagcac agaacgggtg aaaccacgcc ag
//
LOCUS       DAZGJW010000668          412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.675, whole genome shotgun sequence.
ACCESSION   DAZGJW010000668 DAZGJW010000000
VERSION     DAZGJW010000668.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.675"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>412)
                     /locus_tag="ACHKIK_005899"
     CDS             complement(<1..>412)
                     /locus_tag="ACHKIK_005899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9086770.1"
                     /translation="WRGFTRSVLSMLTEIFLKQAMVGIVGSIGSAIGGAFGGGASAST
                     GTAIQAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGY
                     AEGGYVGGAGSSAQMRRTEGINFNQNNHVVIQNDG"
BASE COUNT           92 a          144 c           98 g           78 t
ORIGIN      
        1 gccgtcgttc tgaatcacca cgtgattgtt ctgattaaaa ttaatgcctt ccgtccgccg
       61 catctgcgcc gaacttccgg caccgcccac ataaccacct tccgcatagc cccgcatcag
      121 gcggtacagg ttgccgacgc caatccggct ggttgcttcc ttcgtgaaga caaactcccc
      181 gcgatggaca ataccggcag gttcgtattt gccaccggtt cccgtaaatc ctccggtcgc
      241 gaaatggaag ttcgccgccg cagcctgaat ggccgtcccc gtggaggcag acgcaccacc
      301 accgaaagca ccgccaatgg cgctgccgat actcccgact atccccacca tcgcctgctt
      361 cagaaaaatc tctgtcagca tggagagcac agaacgggtg aaaccacgcc ag
//
LOCUS       DAZGJW010000669          412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.676, whole genome shotgun sequence.
ACCESSION   DAZGJW010000669 DAZGJW010000000
VERSION     DAZGJW010000669.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.676"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>412
                     /locus_tag="ACHKIK_005900"
     CDS             <1..>412
                     /locus_tag="ACHKIK_005900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFO9086771.1"
                     /translation="HHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRL
                     FLPNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILN
                     EKTAGIAIATASILLTKRSNVDTYTEINSYLGKLR"
BASE COUNT          157 a           75 c           71 g          109 t
ORIGIN      
        1 tcatcaccag atcattgaaa accaactgga tacagaaaca ataactatta gttatgaatt
       61 tatcctctgt caacatctta tcgcaaatac cgagttcagc tatttggcac tccctgaaaa
      121 ttataaccgt ttgtttttac caaattcaaa aaaccaaaca aataatcgtt tcaaaacgtt
      181 aaacagcaaa gctattggca ggctacttgc tgctggtggc gtatacaatg ggaacattga
      241 aggattcaga gatactgcgg aaaaactggg tggagatgca ataaaaggct atgatcaaat
      301 actaaatgaa aaaacagcgg gcatagcgat agcaacagca tctattcttt taacaaagcg
      361 ttctaatgtt gatacatata cagaaataaa tagttactta ggcaaactta ga
//
LOCUS       DAZGJW010000670          410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.677, whole genome shotgun sequence.
ACCESSION   DAZGJW010000670 DAZGJW010000000
VERSION     DAZGJW010000670.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.677"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            93..>410
                     /locus_tag="ACHKIK_005901"
     CDS             93..>410
                     /locus_tag="ACHKIK_005901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086772.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIK"
BASE COUNT          137 a           74 c           90 g          109 t
ORIGIN      
        1 tgctgatgat gaattgtgaa taatcaaact gacctctaat tatcctgtat ggagtagtgc
       61 ttattttcga ctcaatttct actgattaga ttatggttat cagtacagtg ggttatgaaa
      121 aacggactgg atctgccagc agtgcttcta ttattattca aaaagaactt cagccggaca
      181 atgctataat ccagggaaag gctataagct ctgttggacg gaatgtggaa atcagcactg
      241 gtaaaagggg aactgacatt aacacaagaa ggattcctgc aagcaactgc ttatctgccc
      301 ggaacgcaga attaaagcat aaccacacag gagccagaat actgtggtcg tacgcattca
      361 gtattgtgac aactaataga aataaaaatc tggccggtgc gaatattaaa
//
LOCUS       DAZGJW010000671          402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.678, whole genome shotgun sequence.
ACCESSION   DAZGJW010000671 DAZGJW010000000
VERSION     DAZGJW010000671.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.678"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>402
                     /locus_tag="ACHKIK_005902"
     CDS             <1..>402
                     /locus_tag="ACHKIK_005902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO9086773.1"
                     /translation="SRPEGFVVYTTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLP
                     VIFEHPPEMVESGAHLLMENLAMVNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSK
                     HANVEIGLALRSDRWAGADFWEQQGRRVSLDD"
BASE COUNT           87 a          103 c          125 g           87 t
ORIGIN      
        1 tcacgtccgg aagggtttgt ggtgtacaca accacccagt cgaatgaacc gcctgccggg
       61 gtgttcaggc agaaactgca gtacgcccgg gatgtccgtg acggcaaaat tcatgatccg
      121 cactttctgc cggtgatatt tgaacaccct cctgaaatgg tggaaagcgg ggctcacctg
      181 ctgatggaaa acctcgccat ggttaacccg aatctcggtt attcggtgga tgaggccttt
      241 ctgtaccggg agtatcgtaa agcccgggaa gccggggaag agactttccg cggtttcatg
      301 tcaaaacacg ccaatgtgga aattggtctt gccctgcgtt ctgaccgctg ggcaggggcg
      361 gatttctggg agcagcaggg caggcgcgtc agcctggacg at
//
LOCUS       DAZGJW010000672          400 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.679, whole genome shotgun sequence.
ACCESSION   DAZGJW010000672 DAZGJW010000000
VERSION     DAZGJW010000672.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.679"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..108
                     /locus_tag="ACHKIK_005903"
     CDS             <1..108
                     /locus_tag="ACHKIK_005903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFO9086774.1"
                     /translation="PEKRPVILEAQDVLITYRGKLPGIITGSLKTPPLA"
     gene            159..>400
                     /locus_tag="ACHKIK_005904"
     CDS             159..>400
                     /locus_tag="ACHKIK_005904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9086775.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAI"
BASE COUNT          109 a           97 c          108 g           86 t
ORIGIN      
        1 ccggaaaagc gtcccgtaat tttagaggcg caggatgtgt tgataaccta cagaggaaaa
       61 ctaccgggaa taattacggg ttctctgaag actccaccgc tggcctgaag acttaacata
      121 tccagggatt tgaaatcgat aaaccctgat aaatatccat gaacgcaaaa atcagatacg
      181 gcctgtcggc tgccgttctg gcgctgattg ccgcaggtgc gcctgcgcct gaaatcctcg
      241 accagtttct ggatgaaaag gaaggtaacc acaccacagc ataccgtgat ggtgcgggta
      301 tctggaccat ctgccgtgga gccacccggg tggatggtaa gcctgttatt cctggcatga
      361 agctgtcgaa ggaaaaatgc gaccgggtta acgccattga
//
LOCUS       DAZGJW010000673          399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.680, whole genome shotgun sequence.
ACCESSION   DAZGJW010000673 DAZGJW010000000
VERSION     DAZGJW010000673.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.680"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>399
                     /locus_tag="ACHKIK_005905"
     rRNA            <1..>399
                     /locus_tag="ACHKIK_005905"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          108 a           94 c          122 g           75 t
ORIGIN      
        1 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
       61 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      121 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      181 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      241 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      301 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      361 cggcctgcgc ggaagatgta acggggctaa accatgcac
//
LOCUS       DAZGJW010000674          396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.681, whole genome shotgun sequence.
ACCESSION   DAZGJW010000674 DAZGJW010000000
VERSION     DAZGJW010000674.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.681"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>396)
                     /locus_tag="ACHKIK_005906"
     CDS             complement(<1..>396)
                     /locus_tag="ACHKIK_005906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO9086776.1"
                     /translation="LEPAYARVFFCALAGQLGISRLTDAVSGDSLTAQEALATLALSG
                     DDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYSGMTGYNGIIARLQQAASDPMV
                     DGILLDMDTPGGMVAGAFDCADIIARVRDI"
BASE COUNT           78 a          134 c          119 g           65 t
ORIGIN      
        1 tatgtcacgc acacgggcga tgatgtcagc gcagtcaaat gcccccgcca ccatcccgcc
       61 gggcgtgtcc atatcgagca gaatgccgtc caccatcgga tcgctggcag cctgttgcag
      121 acgggcgata atgccgttgt aaccggtcat ccccgagtac ggctgcagcg cccgcgtccg
      181 gctgaccagc gtgccggaca ccggcagcac ggcgatgccg ttcatgacct gataactgcg
      241 ggcctgtcgt ggtccgtcat catcaccgga taatgccagc gtcgcgagtg cctcctgggc
      301 agtcaggctg tcgccggaca ccgcatccgt caggcggctg atcccaagct ggcctgcaag
      361 cgcacaaaag aaaacccgcg cataggcggg ttcaag
//
LOCUS       DAZGJW010000675          396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.682, whole genome shotgun sequence.
ACCESSION   DAZGJW010000675 DAZGJW010000000
VERSION     DAZGJW010000675.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.682"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>396)
                     /locus_tag="ACHKIK_005907"
     CDS             complement(<1..>396)
                     /locus_tag="ACHKIK_005907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO9086777.1"
                     /translation="PQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITH
                     LDQDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEW
                     ADYPVSRSFLIAKAASPWSKEDLLKPVNS"
BASE COUNT          121 a           79 c           65 g          131 t
ORIGIN      
        1 tgctatttac aggtttaagt aaatcttctt tactccacgg agaagctgct ttggcgatca
       61 aaaaactacg tgacaccgga tagtcagccc actcatgcca gggttttgta ggtcccacat
      121 agtgtataaa cacggtgtca tcattgactg gattatcaac tttgtctttt aattcatagt
      181 taatactata tcgggtatta tatttctccg aaataaactt cactttacca ttcaataata
      241 cattcagtac atcttgatca aggtgggtga ttttacttac ccaatctggg tcacgcaaca
      301 tttcaatagc ttttgacgaa atgttattta gattccactc atcaatattt atcagtaaaa
      361 aaccagcatt gaaatatcca gaagctaact gtggtg
//
LOCUS       DAZGJW010000676          393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.683, whole genome shotgun sequence.
ACCESSION   DAZGJW010000676 DAZGJW010000000
VERSION     DAZGJW010000676.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.683"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>393
                     /locus_tag="ACHKIK_005908"
     CDS             <1..>393
                     /locus_tag="ACHKIK_005908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO9086778.1"
                     /translation="IKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFT
                     RSKQRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSE
                     NLLEQDFYASGPNQKWAGDITYLRTDEG"
BASE COUNT           87 a          115 c          120 g           71 t
ORIGIN      
        1 catcaaagca atgtgccgcg tgctccgggt ggcccgcagc ggctggtata cgtggtgtca
       61 gcggcggaca aggataagca cgcgtcagca gttccgccaa cactgcgaca gcgttgtcct
      121 cgcggctttt acccggtcaa aacagcgtta cggtgcccca cgcctgacgg atgaactgcg
      181 tgctcagggt taccccttta acgtaaaaac cgtggcggca agcctgcgcc gtcagggact
      241 gagggcaaag gcctcccgga agttcagccc ggtcagctac cgcgcacacg gcctgcctgt
      301 gtcagaaaat ctgttggagc aggattttta cgccagtggc ccgaaccaga agtgggcagg
      361 agacatcacg tacttacgta cagatgaagg ctg
//
LOCUS       DAZGJW010000677          390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.684, whole genome shotgun sequence.
ACCESSION   DAZGJW010000677 DAZGJW010000000
VERSION     DAZGJW010000677.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.684"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..266
                     /locus_tag="ACHKIK_005909"
     CDS             <1..266
                     /locus_tag="ACHKIK_005909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086779.1"
                     /translation="SAPHELANILTREKILTTREKKGNLISLFDGFTLFHCVGMITFL
                     IHYLRTPEEKVENIFMLYGADKNNKLRRRLIYDALGIIQSQQE"
BASE COUNT          144 a           68 c           66 g          112 t
ORIGIN      
        1 tatcagcccc ccatgaactg gcaaatattc ttacccgcga aaaaatactt acaacacggg
       61 aaaaaaaagg aaacctgata agcctgtttg atggattcac tttattccat tgtgttggaa
      121 tgataacctt tcttatccat tatctcagaa cacctgaaga aaaagttgaa aatatattta
      181 tgttatatgg tgcagataaa aacaataaac tacgcagaag actgatttat gacgcactag
      241 gaataattca gtctcagcag gagtgaagag ttaaacaggc aaattatttt ttataaaaag
      301 gggattgtta agtaatcccc tgttaatcaa aatacgcttt cctgacgatc tgataattat
      361 cagctcaata catttgacat aaaagcggtt
//
LOCUS       DAZGJW010000678          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.685, whole genome shotgun sequence.
ACCESSION   DAZGJW010000678 DAZGJW010000000
VERSION     DAZGJW010000678.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.685"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>389
                     /locus_tag="ACHKIK_005910"
     CDS             <1..>389
                     /locus_tag="ACHKIK_005910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO9086780.1"
                     /translation="AGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVAR
                     KDLKAMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGAN
                     EASGVTKTLSGMLNGVAGQIDNVATAVG"
BASE COUNT           90 a           81 c          138 g           80 t
ORIGIN      
        1 gcgggctcgg tgatcaccca gttcagtcag gcgctggcgc agggtgtatt gcgtggtgag
       61 gagtttaatt cggtcaatga aagtggtgac cggatcgtac gtgcactggc tgcgggtatg
      121 ggcgtggccc gtaaagatct gaaggcaatg gcggatgacg gaaaactgac agcggataaa
      181 gtggtccccg cgttaatcag ccagctgggg atattacgtg atgaatatgc ggccatgccg
      241 gaaacggttt ccagtagtat cacaaaggtg gaaaacgcct ttatggcctg ggtgggcggt
      301 gcgaatgagg ccagcggggt gacaaaaacg ctctccggca tgctgaacgg tgttgccgga
      361 cagattgata atgtggcaac agccgtggg
//
LOCUS       DAZGJW010000679          386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.686, whole genome shotgun sequence.
ACCESSION   DAZGJW010000679 DAZGJW010000000
VERSION     DAZGJW010000679.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.686"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            13..>386
                     /locus_tag="ACHKIK_005911"
     CDS             13..>386
                     /locus_tag="ACHKIK_005911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleG8"
                     /protein_id="HFO9086781.1"
                     /translation="MPVTTLSIPSISQLSPAGVQSLQDAARLESGIRISIGSGQYSVH
                     YVQLLDGFSVEPVRGGLLDRLLGREHRMERRAVALERQLNGGVDFLSSVNNYFQSVMA
                     EHRENKTSNKILMEKINSCLFRP"
BASE COUNT          121 a           57 c           93 g          115 t
ORIGIN      
        1 aatgaggttt ttatgcctgt taccacctta agtatcccaa gtatatctca attatctcct
       61 gcaggagtac agtctttgca ggatgctgcc agacttgaaa gtggaataag aatatccatt
      121 ggtagtggcc aatattctgt tcactatgtc cagctactgg atggattttc agttgaaccg
      181 gtgagaggag gcttactgga taggctattg gggcgtgagc atcgaatgga gagaagggct
      241 gtggctctgg aaaggcaatt aaatggaggt gtcgattttt taagtagtgt taataactat
      301 tttcagagtg tcatggcaga acacagagaa aataaaacaa gtaataaaat attaatggaa
      361 aaaataaatt cttgtttatt tagacc
//
LOCUS       DAZGJW010000680          385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.687, whole genome shotgun sequence.
ACCESSION   DAZGJW010000680 DAZGJW010000000
VERSION     DAZGJW010000680.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.687"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>385
                     /locus_tag="ACHKIK_005912"
     CDS             <1..>385
                     /locus_tag="ACHKIK_005912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086782.1"
                     /translation="GNVGYNESRYAFNFKVDNNEDYKSLISATLGYPTRINVKDVRGW
                     DTRFDINFQMEELTAEQAIIEIRSVEAFSTGSIIFEDEKEDVSFKCEFFVSPIALNAP
                     RDLASFRICSDFFDLLVGIKNNKLKI"
BASE COUNT          129 a           56 c           77 g          123 t
ORIGIN      
        1 ggtaatgtag gatataacga aagccgctat gcattcaatt tcaaagttga taataatgag
       61 gattataagt ctcttattag tgccacgctt ggatatccga ccagaataaa tgtaaaagat
      121 gttcgcggtt gggatactcg atttgatatt aattttcaga tggaagaact gacagctgaa
      181 caagcaataa tcgaaataag atctgttgaa gcattctcaa caggctctat aatattcgaa
      241 gatgaaaaag aagatgtctc ttttaagtgt gaatttttcg tttctcctat tgcacttaat
      301 gctccaaggg atctggcaag ctttagaatt tgctcggact ttttcgattt actggttggt
      361 attaaaaata acaaattgaa aatag
//
LOCUS       DAZGJW010000681          385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.688, whole genome shotgun sequence.
ACCESSION   DAZGJW010000681 DAZGJW010000000
VERSION     DAZGJW010000681.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.688"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>385)
                     /locus_tag="ACHKIK_005913"
     CDS             complement(<1..>385)
                     /locus_tag="ACHKIK_005913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO9086783.1"
                     /translation="LNSSLIYYINNWNLFPLFCLFSGYHFYRERFAERGFFYKVPAVL
                     RDYLSAIPVKINEKARYKPGIASYHNIITCGFSTLSPYIRQQPLAMQQRFNLLFPDFV
                     DHIQLPLPLASTLLERITFYAKKNR"
BASE COUNT          131 a           63 c           69 g          122 t
ORIGIN      
        1 tctctatttt ttttcgcata aaatgtaatt ctctctaaaa gagttgatgc aagtggtaac
       61 ggcaattgta tgtgatcaac aaaatcagga aataataaat taaatcgttg ctgcattgcc
      121 aatggttgtt gccttatata gggtgacaac gttgaaaaac cacatgtgat gatattgtga
      181 tagctagcga tgccgggttt gtatcgtgcc ttttcattta ttttcaccgg tatagctgat
      241 aaataatccc ttagcacagc aggaacctta taaaaaaatc cgcgttctgc aaacctttct
      301 cgataaaagt gataaccact gaataaacag aacaatggaa ataaattcca attgtttatg
      361 tagtaaataa gtgaactatt cagat
//
LOCUS       DAZGJW010000682          383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.689, whole genome shotgun sequence.
ACCESSION   DAZGJW010000682 DAZGJW010000000
VERSION     DAZGJW010000682.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.689"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>383
                     /locus_tag="ACHKIK_005914"
     CDS             <1..>383
                     /locus_tag="ACHKIK_005914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086784.1"
                     /translation="TIHITDESGDYDTFDFRNIVFEHHTNDMFISFDNQGGFVISISN
                     NATNELADINVFRKNMTSFDGCRSLIYLPGGDVFYISDIYKMSRGRKSVKLNVEKKPD
                     INDIINVAILETSYLQIKKIPNNDD"
BASE COUNT          138 a           57 c           62 g          126 t
ORIGIN      
        1 taaccattca cattactgat gaaagtggcg attatgatac ttttgacttc cgcaatattg
       61 tttttgaaca tcacaccaat gacatgttta taagctttga taatcaagga ggatttgtta
      121 tatcaatatc aaataatgcc acaaatgaac tggcagatat taatgtattc cgaaaaaaca
      181 tgacttcttt tgatggctgt cgttcattga tatatttgcc tggcggtgat gttttttata
      241 tttcagatat ctataaaatg tccagaggaa gaaaatcggt taaattgaat gtagaaaaga
      301 aaccggacat taacgacatc atcaatgtag ctattttaga aacttcttat ctgcaaataa
      361 aaaaaatccc aaataatgat gat
//
LOCUS       DAZGJW010000683          377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.690, whole genome shotgun sequence.
ACCESSION   DAZGJW010000683 DAZGJW010000000
VERSION     DAZGJW010000683.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.690"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>377
                     /gene="espW"
                     /locus_tag="ACHKIK_005915"
     CDS             <1..>377
                     /gene="espW"
                     /locus_tag="ACHKIK_005915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFO9086785.1"
                     /translation="SSVVSSCYHLFSEHQQLSNETTMTNSVSRRIVHKEYGISLKSVP
                     VWLATAKTPLALLNGRHTRSHSFIIAGTPGMGSRSGAQYYAINSDDKRSRIDIDSLFL
                     KKLNNARNQNKFPIDVKETVIKL"
BASE COUNT          136 a           76 c           62 g          103 t
ORIGIN      
        1 tatcatcagt tgtatcatca tgttaccatc tgttcagtga acatcaacaa ctttcaaatg
       61 aaacaacaat gacgaactcc gtctccagaa gaattgttca taaagaatat ggtatatctt
      121 taaaatccgt tcctgtatgg ttggctacag ctaaaactcc ccttgctcta ctcaatggca
      181 gacatacaag aagtcactca tttattattg cagggactcc aggaatggga agcaggagcg
      241 gagcccaata ctatgccata aacagtgatg ataaacgctc ccgcatagac attgactctt
      301 tatttttaaa aaagttaaat aatgcgcgaa atcaaaataa atttccaatc gatgtaaaag
      361 aaacggttat aaaacta
//
LOCUS       DAZGJW010000684          375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.691, whole genome shotgun sequence.
ACCESSION   DAZGJW010000684 DAZGJW010000000
VERSION     DAZGJW010000684.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.691"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..293)
                     /locus_tag="ACHKIK_005916"
     CDS             complement(<1..293)
                     /locus_tag="ACHKIK_005916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO9086786.1"
                     /translation="MAFLLNKSDMASSIGISVQAFDKWGVPPVERRGREVFYDVKTVL
                     EIDRERRQHNQRIPDDEGDLEERLLRARAELTEEQAVAQKLKNQVTEGKLIDTG"
BASE COUNT           97 a          102 c           64 g          112 t
ORIGIN      
        1 ccggtgtcaa taagcttacc ttcggttacc tgatttttaa gtttttgagc tacggcctgt
       61 tcttctgtca gttcagctct ggcccgaagc agcctttcct ccagatcgcc ctcgtcatca
      121 ggtattctct gattgtgttg tcgccgctcg cgatctatct ccagtacagt tttaacgtca
      181 taaaaaactt ccctcccccg acgctcaaca ggaggaacgc cccatttatc aaatgcctga
      241 acagatatac cgatggagga ggccatatca cttttattca ataaaaaggc catctcctct
      301 ccataagcca tcgataaaaa gcgatacaac aaccatgtgt ttttacaaaa ccatttgata
      361 tcattgacat ttttc
//
LOCUS       DAZGJW010000685          371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.692, whole genome shotgun sequence.
ACCESSION   DAZGJW010000685 DAZGJW010000000
VERSION     DAZGJW010000685.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.692"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>371)
                     /locus_tag="ACHKIK_005917"
     CDS             complement(<1..>371)
                     /locus_tag="ACHKIK_005917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086787.1"
                     /translation="NDIPESPTGNNLEALAPGIEKPKQTSIEMVTLLNTLQPDGKCII
                     TGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSDNTCELTINEPLKNQKISTGS
                     INVNCPLKYIYNEIRRLNVIF"
BASE COUNT          105 a           57 c           72 g          137 t
ORIGIN      
        1 aaagattacg ttcaacctcc tgatttcatt gtaaatatat ttcaatggac aattaacatt
       61 gatactgcct gtggatattt tctggttctt taatggttca ttaatggtaa gttcgcaggt
      121 attgtccgat cgttgaataa tttgggcgtt ataacctaaa aaatccagac gaagagacga
      181 gtcattataa agaattacat tttgtaagta cgctaattct ttttgaaaat caccagtgat
      241 aatgcatttt ccatcaggtt gtaacgtgtt aagtaaagtg accatttcaa tagaggtctg
      301 ttttggtttt tctatacctg gtgctagtgc ttcgaggtta tttcctgttg ggctttcagg
      361 aatgtcattc a
//
LOCUS       DAZGJW010000686          371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.693, whole genome shotgun sequence.
ACCESSION   DAZGJW010000686 DAZGJW010000000
VERSION     DAZGJW010000686.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.693"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            81..>371
                     /gene="grlR"
                     /locus_tag="ACHKIK_005918"
     CDS             81..>371
                     /gene="grlR"
                     /locus_tag="ACHKIK_005918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HFO9086788.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNLITGGDIASVYQGVL
                     SEEEDIILHVHRYNHEIPSVLNIEQDYQLVIPKKVLSNDSNVTLHCHVRGNEK"
BASE COUNT          124 a           51 c           83 g          113 t
ORIGIN      
        1 ataggtctcc gttattcttg atatcgctta tggatagaac aaattgaaag gagtgaggtt
       61 agtatgaaac tgagtgagtt atgattatga aggatggcat ctatagcatt atctttatta
      121 gcaatgaaga ttcctgtggg gaaggtatac tgattaaaaa tggaaatttg atcactggcg
      181 gagatattgc ttctgtgtat cagggggtcc tgtctgaaga ggaggacatc atacttcatg
      241 tccatcgcta taatcatgaa attccctcgg tgctaaacat tgaacaagat tatcaattag
      301 ttatccctaa aaaagtactg agtaatgata gtaatgtcac attacattgc catgtaagag
      361 gaaatgaaaa a
//
LOCUS       DAZGJW010000687          366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.694, whole genome shotgun sequence.
ACCESSION   DAZGJW010000687 DAZGJW010000000
VERSION     DAZGJW010000687.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.694"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..311
                     /locus_tag="ACHKIK_005919"
     CDS             <1..311
                     /locus_tag="ACHKIK_005919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086789.1"
                     /translation="IRAGVQYYDRQTKKKEWTNYSAVVFAKPGAQADYYRSVLVEGGI
                     VEITGENIRVDVYQGQNGQSITLELLNAKIGFAASGNGPQQQSSNQQNTPVYDDSIPF
                     "
BASE COUNT          112 a           75 c           90 g           89 t
ORIGIN      
        1 acatccgtgc tggcgttcag tattacgatc gccagacaaa aaagaaagaa tggacaaact
       61 acagcgccgt tgtatttgcc aagccgggag cgcaagcgga ttactaccgt agtgttcttg
      121 ttgaaggtgg cattgtggaa attaccggag aaaacatcag ggttgatgtt tatcaggggc
      181 aaaatggtca atcaatcact cttgaattac tgaatgcaaa gattggattt gcagcttcag
      241 gaaatggccc gcagcagcaa agtagtaacc agcagaacac tcctgtatac gacgattcca
      301 tcccattctg atttagaaaa ataaggattt aattatgcca gcgcctctgt atggtgcgga
      361 tgacgc
//
LOCUS       DAZGJW010000688          365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.695, whole genome shotgun sequence.
ACCESSION   DAZGJW010000688 DAZGJW010000000
VERSION     DAZGJW010000688.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.695"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..140)
                     /locus_tag="ACHKIK_005920"
     CDS             complement(<1..140)
                     /locus_tag="ACHKIK_005920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO9086790.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DER"
     gene            complement(230..>365)
                     /locus_tag="ACHKIK_005921"
     CDS             complement(230..>365)
                     /locus_tag="ACHKIK_005921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO9086791.1"
                     /translation="SGELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK
                     "
BASE COUNT           84 a           77 c          115 g           89 t
ORIGIN      
        1 cgttcatcgg cgaacggtgt gtcattcagt gtaaggccgt aatgctggcc cagtagtcgg
       61 gagagcagag tctgccagat ggtgacggga gacgggcagc tcgatgcctc ccgtacgtgt
      121 gtatcgggta atgttttcat actgaaggtt ttcctgatat gcagagatga aaaatggaaa
      181 agtggcgtgg tgaaagcacc aggccggagc agaaggttat tccggtggtt tactttttcg
      241 tttcgggcgt cggataaaca gccagataaa cgtaaccacc gctaccgaga gtgtcagctt
      301 tgcaggtcag accttttgca tacagcgtga cggtatgctg atggcgggga ttcagttcac
      361 cgctg
//
LOCUS       DAZGJW010000689          360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.696, whole genome shotgun sequence.
ACCESSION   DAZGJW010000689 DAZGJW010000000
VERSION     DAZGJW010000689.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.696"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            169..>360
                     /locus_tag="ACHKIK_005922"
     CDS             169..>360
                     /locus_tag="ACHKIK_005922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9086792.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGT"
BASE COUNT           90 a           89 c           89 g           92 t
ORIGIN      
        1 aaaatcggcc agttttctct gcctgcagtc cgcatgccgt atcgggcctt gggttctaac
       61 ctgttgcgta gatttatgca gcggactgcc tttctcccaa agtgataaac cggacagtat
      121 catggaccgg ttttcccggt aatccgtatt tgcaaggttg gtttcactat ggaacatgaa
      181 cttcattata tcggtatcga caccgctaaa gagaaactgg atgtcgatgt gttgcgtcct
      241 gatggtcgtc atcgcaccaa aaaattcgct aacaccacta aagggcacga tgagctggtg
      301 agctggctga aaggtcacaa gattgaccat gcgcatatct gcatcgaagc gaccggcacc
//
LOCUS       DAZGJW010000690          358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.697, whole genome shotgun sequence.
ACCESSION   DAZGJW010000690 DAZGJW010000000
VERSION     DAZGJW010000690.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.697"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..193
                     /locus_tag="ACHKIK_005923"
     CDS             <1..193
                     /locus_tag="ACHKIK_005923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086793.1"
                     /translation="TTSSAGEHAHNVYIGAHNHTIGIGAHAHSVIIGPHGHTITVNAT
                     GNEENTVKNIAFNYIVRLA"
     gene            193..>358
                     /locus_tag="ACHKIK_005924"
     CDS             193..>358
                     /locus_tag="ACHKIK_005924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO9086794.1"
                     /translation="MTFRMSEHSRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFV"
BASE COUNT          105 a           93 c           73 g           87 t
ORIGIN      
        1 taccacgtcg agcgcaggcg agcatgctca caatgtatat atcggtgccc ataaccacac
       61 tatcggcatt ggtgctcatg cccattctgt cattattggt ccccacggac acaccatcac
      121 cgttaacgct acgggtaacg aagaaaacac cgtaaaaaac atcgcattta actatattgt
      181 gaggcttgca taatgacatt cagaatgagt gaacactcac ggaccataaa aatttataat
      241 ctactgaccg gaaccaatga gtttattggt gaaggtgatg catacattcc acctcataca
      301 ggtctgcctg caaacagtac cgatattgcc ccgccagata ttccggctgg cttcgtgg
//
LOCUS       DAZGJW010000691          356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.698, whole genome shotgun sequence.
ACCESSION   DAZGJW010000691 DAZGJW010000000
VERSION     DAZGJW010000691.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.698"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>356
                     /locus_tag="ACHKIK_005925"
     CDS             <1..>356
                     /locus_tag="ACHKIK_005925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9086795.1"
                     /translation="TEMQIAAEARLAATQERLNRNIAARSAAQNALNSTTAVGSRLMS
                     GALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALTIDEIAHKTPSMSLPEASD
                     NEGRTRAALTEQNRLI"
BASE COUNT           87 a           90 c          120 g           59 t
ORIGIN      
        1 acggagatgc agattgctgc agaggcccgt ctggcggcca cacaggaacg cctgaacaga
       61 aatattgctg ccagaagcgc cgcccagaat gcgctgaaca gtacaacggc ggtgggctca
      121 cgtctgatga gcggtgcgct ggggctggtt ggtggcgtac ccggactggt gatgctgggg
      181 gctgcagcat ggtacacgct gtaccagaat caggagcagg ccagggagtc tgcgcgccag
      241 tatgcactga cgatagatga aatcgcgcat aaaacgccgt caatgtcttt gcctgaagcc
      301 tcagataatg aaggacgaac acgggcggcg ctgacagagc agaaccggct gattga
//
LOCUS       DAZGJW010000692          353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.699, whole genome shotgun sequence.
ACCESSION   DAZGJW010000692 DAZGJW010000000
VERSION     DAZGJW010000692.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.699"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT           91 a           92 c           69 g          101 t
ORIGIN      
        1 aactccgctg tcaatcatga gcaaaattta ctctgtggcg agataaaact ccggccttac
       61 cgggttatcc ccaaagcaac ggctttttta acatttgtca agatgagtga tgacagtttt
      121 atggcaagag atgcctgttc agtgactgcc attgcattta tctaaccagt taaaaaataa
      181 aagatatttc tttgagccgt ctttaacgcc aattcgcgtg caagccgcgt attctctcgc
      241 ttgcctcgtg ttttctaact ctaatacaca tggttatcca caggaatagt ggataactgt
      301 ctccagcccc tatcccccgc cgcctgggca attcccacat tcgccgcatg agg
//
LOCUS       DAZGJW010000693          349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.700, whole genome shotgun sequence.
ACCESSION   DAZGJW010000693 DAZGJW010000000
VERSION     DAZGJW010000693.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.700"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..245)
                     /locus_tag="ACHKIK_005926"
     CDS             complement(<1..245)
                     /locus_tag="ACHKIK_005926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9086796.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQVYPIDGSGFEMNGKGSSARPSLTVS"
     gene            complement(245..>349)
                     /locus_tag="ACHKIK_005927"
     CDS             complement(245..>349)
                     /locus_tag="ACHKIK_005927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9086797.1"
                     /translation="PYAWRQIKVTCAAWSSRVRMLRVEFSAEFKQVVN"
BASE COUNT           76 a          122 c           71 g           80 t
ORIGIN      
        1 gacaccgtca gcgacggtct ggcactgctg cccttcccgt tcatctcaaa accgctgccg
       61 tcaatcgggt atacctgata ttgccgcccc tgccaggtga ccgcctcccc tttttcattc
      121 agctcattac agaaaaaata ccgctcaccg ccctgcaccg tcaggtcaat ttcccagagc
      181 accacccgcg gtgactgctc tgatttaacc gactcgttca gactttcttc gtgaatatcc
      241 tgcatcagtt caccacctgc ttaaactcgg cactgaattc aacccgcagc atgcgaaccc
      301 gtgatgacca ggcggcacag gtcaccttta tctgccgcca ggcataagg
//
LOCUS       DAZGJW010000694          344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.701, whole genome shotgun sequence.
ACCESSION   DAZGJW010000694 DAZGJW010000000
VERSION     DAZGJW010000694.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.701"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..221)
                     /locus_tag="ACHKIK_005928"
     CDS             complement(<1..221)
                     /locus_tag="ACHKIK_005928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir chaperone family protein"
                     /protein_id="HFO9086798.1"
                     /translation="MNEQFRTDLAHTFGITLEEQTNALSFHDHDGHEWILECAPQSEV
                     LFFYCYLFNNESSKIDKILEINSNRDLLG"
BASE COUNT          113 a           53 c           64 g          114 t
ORIGIN      
        1 ataccgagta gatctcgatt gctatttatt tctagtattt tatcaatttt tgaagattca
       61 ttattgaata aataacaata aaaaaacagg acctcagatt gaggtgcaca ctccagtatc
      121 cattcatgac catcgtgatc atggaaagat agggcgttag tttgttcttc aagagttatt
      181 ccgaacgtat gtgcaagatc tgtgcgaaat tgttcattca tattgtagtc ctgtcattct
      241 ttcaatgagg gtatgcttaa acgtaattaa ctcgtgagct ctggcacaaa taataagacg
      301 aaaatataag atgcaagtaa tagtgttgca tatttatgat agca
//
LOCUS       DAZGJW010000695          344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.702, whole genome shotgun sequence.
ACCESSION   DAZGJW010000695 DAZGJW010000000
VERSION     DAZGJW010000695.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.702"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..259
                     /locus_tag="ACHKIK_005929"
     CDS             <1..259
                     /locus_tag="ACHKIK_005929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO9086799.1"
                     /translation="EKTLTVNTGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTF
                     SKANTQSGDKGAYTCEVTDSAEQPQSITSDACTVTVNGAGG"
     gene            265..>344
                     /locus_tag="ACHKIK_005930"
     CDS             265..>344
                     /locus_tag="ACHKIK_005930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO9086800.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEW"
BASE COUNT           96 a           74 c          108 g           66 t
ORIGIN      
        1 ggagaagaca cttaccgtga ataccggtgc gctgctgaca atgtcagtca gtgtcaacgg
       61 gggaacgccg ccttataaac acgcctggaa gaaggatggt cagccggtag agggacagac
      121 tactgacact ttcagtaaag ccaatacgca gtcaggtgat aagggggctt atacctgcga
      181 ggtaacggat tctgcagaac agccgcagag cattacctct gatgcgtgta cagtaacggt
      241 taatggtgcg ggcggataag gcttatggca aaagatctga aaacactggc gctggccaga
      301 ctgtcggggt tccgtcataa aacggtgaag gtgccggaat ggag
//
LOCUS       DAZGJW010000696          340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.703, whole genome shotgun sequence.
ACCESSION   DAZGJW010000696 DAZGJW010000000
VERSION     DAZGJW010000696.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.703"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..315
                     /locus_tag="ACHKIK_005931"
     CDS             1..315
                     /locus_tag="ACHKIK_005931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO9086801.1"
                     /translation="MLHDHLAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     KC"
BASE COUNT          117 a           63 c          100 g           60 t
ORIGIN      
        1 atgctacatg atcacctggc agaatgtctg gagaaaaaag gactgtaccg gagagcagct
       61 gaacgatggg caaaagtgat ggtacagcta agtgatgacc agaaaagaaa agtggcggca
      121 cagaaacgag cagagtgttt gcgtaaggcg cgccggactc cggtttcacc ggtgaacctg
      181 accgaaataa aacaagcggt caacagacta cattctgagt tgggaatggg atttgaagag
      241 cggcgggtat tccgacgata taaagggaca ggagaacaga atacgtccgg aaacgcgcgg
      301 tcaaaaaaat gctaaaaaat atctgagaga gttattgcct
//
LOCUS       DAZGJW010000697          335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.704, whole genome shotgun sequence.
ACCESSION   DAZGJW010000697 DAZGJW010000000
VERSION     DAZGJW010000697.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.704"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>335)
                     /locus_tag="ACHKIK_005932"
     CDS             complement(<1..>335)
                     /locus_tag="ACHKIK_005932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO9086802.1"
                     /translation="YQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGY
                     TLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYL
                     SDHPKLQGIA"
BASE COUNT           78 a           96 c           91 g           70 t
ORIGIN      
        1 gcaataccct gcagtttcgg gtgatcgctg agatatttca gggaggcttt gtagtcttcc
       61 agcaacagtt cagcaaagtc catttcgaag ccgcgacgac acataatgcg catcaccacg
      121 atgtcggtgg cttcaccgcc gagagtgaag gccggaacct gccagccgcg cagacgcaga
      181 cgttcagaga ggtcgtacag ggtgtatccc ggatcttcac catctttcag tttgaagcaa
      241 accgccggga tgccttcgtc cgggcgaccc gtacagatga actcatacgg ccccagtttg
      301 gcgatttcat ccgccagata agcggcaacc tggta
//
LOCUS       DAZGJW010000698          333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.705, whole genome shotgun sequence.
ACCESSION   DAZGJW010000698 DAZGJW010000000
VERSION     DAZGJW010000698.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.705"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>333)
                     /locus_tag="ACHKIK_005933"
     CDS             complement(<1..>333)
                     /locus_tag="ACHKIK_005933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltR family transcriptional regulator"
                     /protein_id="HFO9086803.1"
                     /translation="NDLNKLNGYLDSLDERGLILSLAAFSEDALGKMLLTFMLDNKAS
                     KELIEGFNAPLGTFSSRIKACFSLGLITEGQYKDLELLRKIRNKFSHSWENISIEDQD
                     ISQQIKALS"
BASE COUNT           95 a           63 c           61 g          114 t
ORIGIN      
        1 gcttaacgct ttaatttgct gagaaatatc ctgatcctca attgaaatgt tttcccagct
       61 gtgagagaat ttatttctta tttttctaag aagttcaaga tctttgtatt gaccctctgt
      121 tattagtccc aaggaaaaac aggcttttat tcgtgaagaa aatgttccaa ggggggcatt
      181 aaacccttcg attaattctt tagacgcttt gttatcgagc atgaacgtca aaagcatttt
      241 acctaaggcg tcttcagaga aagccgctag agacaatatt aggcctcttt catctaggct
      301 gtccaaataa ccgttcagtt tgtttagatc att
//
LOCUS       DAZGJW010000699          331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.706, whole genome shotgun sequence.
ACCESSION   DAZGJW010000699 DAZGJW010000000
VERSION     DAZGJW010000699.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.706"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..198
                     /gene="cbtA"
                     /locus_tag="ACHKIK_005934"
     CDS             <1..198
                     /gene="cbtA"
                     /locus_tag="ACHKIK_005934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HFO9086804.1"
                     /translation="DAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATGLM
                     TRDNYRTVNNITLGKYQEAKR"
     gene            195..>331
                     /locus_tag="ACHKIK_005935"
     CDS             195..>331
                     /locus_tag="ACHKIK_005935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO9086805.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLTELINNVAENGYS
                     LR"
BASE COUNT           87 a           83 c           86 g           75 t
ORIGIN      
        1 gatgcagtga actttcttgt tgaaaaatac gcactggtgc gtaccgacca gccggaattc
       61 agtgcctgta cccgctctca gttaataaac agcatcgata tcctccgggc tcgccgggcg
      121 actggcctga tgacccgcga caattaccga acggtaaata acattaccct gggtaagtat
      181 caggaggcga aacgatgaaa ctggccctga cgctggaagc tgacagcgtt aacgtgcagg
      241 cactgaacat ggggcgcatt gtcgttgacg tcgatggtgt taatctcact gaactgatta
      301 ataacgtcgc tgaaaacggt tattcactcc g
//
LOCUS       DAZGJW010000700          330 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.707, whole genome shotgun sequence.
ACCESSION   DAZGJW010000700 DAZGJW010000000
VERSION     DAZGJW010000700.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.707"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>330)
                     /locus_tag="ACHKIK_005936"
     CDS             complement(<1..>330)
                     /locus_tag="ACHKIK_005936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO9086806.1"
                     /translation="GQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVRAYKIRFPNGT
                     VDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITPVTAIKVTPTSGTVA
                     KGKTTTLT"
BASE COUNT           60 a          111 c           70 g           89 t
ORIGIN      
        1 gtcagggttg ttgttttccc ttttgccacc gtaccagatg tcggcgtcac cttaatcgca
       61 gtgaccggcg taatttcgct gcgttcttcc gccacagaag gtttacccac gttggtcact
      121 ttcaccgtac gggtgatcac ttccttcgcc gttacggcct taccgatact gctgacccag
      181 ccacggaaca catccaccgt gccgttcggg aaacggattt tataggcccg cacatccccg
      241 ctttcaaacc agcctataag ccctttctga ccttcttctc ccggtttcca ggccagcgta
      301 aaactggtat ctcctgcaga cttctgcccc
//
LOCUS       DAZGJW010000701          329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.708, whole genome shotgun sequence.
ACCESSION   DAZGJW010000701 DAZGJW010000000
VERSION     DAZGJW010000701.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.708"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..101)
                     /locus_tag="ACHKIK_005937"
     CDS             complement(<1..101)
                     /locus_tag="ACHKIK_005937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO9086807.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNTPINNPVL"
     gene            complement(117..>329)
                     /locus_tag="ACHKIK_005938"
     CDS             complement(117..>329)
                     /locus_tag="ACHKIK_005938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="required for invasion of epithelial cells; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFO9086808.1"
                     /translation="SVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
BASE COUNT          106 a           61 c           54 g          108 t
ORIGIN      
        1 ctaagcaccg gattgttaat aggtgtatta ttcaagctta ctcgttgagg atgaatataa
       61 ctaattggat catatataat ctttcttagg gcaagattca taacgctctc atatgtctac
      121 ttaattttca acctgactaa attagttaga atggccctat acttccataa cagccagcaa
      181 gtcgctacgg atattaatgc aagtaagata gaaaccggca tagccttatc ataagcaaaa
      241 acaggttcgc taatttcata tgttggtgct tgctcaataa tgtctcttcg ttttgacaat
      301 acaacagaaa tattttcata ttgtacgct
//
LOCUS       DAZGJW010000702          328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.709, whole genome shotgun sequence.
ACCESSION   DAZGJW010000702 DAZGJW010000000
VERSION     DAZGJW010000702.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.709"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..195
                     /locus_tag="ACHKIK_005939"
     CDS             <1..195
                     /locus_tag="ACHKIK_005939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO9086809.1"
                     /translation="DAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATGLM
                     TRDNYRTVNNITLGKHPEKR"
     gene            192..>328
                     /locus_tag="ACHKIK_005940"
     CDS             192..>328
                     /locus_tag="ACHKIK_005940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO9086810.1"
                     /translation="MKLSLMLEADRINVQALNMGRIVVDVDGVNLTELINKVAENGYS
                     LR"
BASE COUNT           88 a           79 c           85 g           76 t
ORIGIN      
        1 gatgcggtga attttctcgt ggaaaaatac gcgctggtgc gtaccgacca gccgggattc
       61 agcacttgtc cccgctctca gttaataaac agtattgata tcctccgggc tcgcagggcg
      121 accggcctga tgacccgcga caattacaga acggtaaata acattaccct gggtaagcat
      181 ccggagaaac gatgaaactt tccctgatgc tggaagccga cagaattaat gtgcaggcac
      241 tgaacatggg gcgaattgtc gttgacgtcg atggtgttaa tctcactgaa ctgattaaca
      301 aggtcgctga aaacggttat tcactccg
//
LOCUS       DAZGJW010000703          326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.710, whole genome shotgun sequence.
ACCESSION   DAZGJW010000703 DAZGJW010000000
VERSION     DAZGJW010000703.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.710"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..312
                     /locus_tag="ACHKIK_005941"
     CDS             <1..312
                     /locus_tag="ACHKIK_005941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO9086811.1"
                     /translation="PPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFTG
                     SGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTFN
                     W"
BASE COUNT           74 a           90 c           80 g           82 t
ORIGIN      
        1 ccaccgtttg caacccttgc cggcatacgc tgcagtaccg cacatatcac ggaaaaggat
       61 aacgcctggc tgtactcgct gtcgcatcag accagtgacg ttggtgaatc agaatggatt
      121 catttcacag gtagcggata tctgttacgt accgatgcgt ggtcatatcc ggttctgcgg
      181 cttaaacgcc tggggctgtc aaaaacgttc cgtcgtctgg ttatcacact tacccgacgt
      241 tatggcgtca gtctcattca tctggatgcc agcgctgaat gcctgccggg tttacccact
      301 ttcaactggt aaccaggaac aacatg
//
LOCUS       DAZGJW010000704          325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.711, whole genome shotgun sequence.
ACCESSION   DAZGJW010000704 DAZGJW010000000
VERSION     DAZGJW010000704.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.711"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            216..325
                     /locus_tag="ACHKIK_005942"
                     /pseudo
     CDS             216..325
                     /locus_tag="ACHKIK_005942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           72 a           99 c           62 g           92 t
ORIGIN      
        1 tccctaatct cacataggat cagatctcgt ctgatcgttc cccctgcgct cactaccatg
       61 gctctttacc acagcagccg gggttgtttg ggaccgactt ccgacagcag atcccggtgg
      121 gcctgccacc atctcttcac aagcttatgc ttattacatg caaaacgtcc cgttttgcat
      181 ctttgtgcct acggcatccc tgtgatcttt ccctgaatct gcccctgaga acctgaatca
      241 aacgtctgac tcgcagaacc atctgtttct cgcgctctgt agaacttcat gaaaaagtca
      301 tcggtgcttt tatcgaaaaa tacat
//
LOCUS       DAZGJW010000705          323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.712, whole genome shotgun sequence.
ACCESSION   DAZGJW010000705 DAZGJW010000000
VERSION     DAZGJW010000705.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.712"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..266
                     /locus_tag="ACHKIK_005943"
     CDS             <1..266
                     /locus_tag="ACHKIK_005943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO9086812.1"
                     /translation="APWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTDRFG
                     QVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
BASE COUNT           63 a           85 c           92 g           83 t
ORIGIN      
        1 ttgccccctg gttaccccgt gatgaaacaa cggcgctcac cgaggaggca ttcatcgggt
       61 gtatggccac catcaccggt tatcacgcag tggcgggttc cccctgtgaa ggtcgcttca
      121 cagaccgctt tgggcaggtt cattacctgt tgctggagcc ggaggccggg aaagaattca
      181 gacgaggaga caaagtgatt attgtctgcc gtctttccgg ttcccgctgg ctgggcgaac
      241 tgaatccctg gccggatgtt ctttaatccc gacatataca gaggttctca ctatgtttaa
      301 tcttccggga tggttgtagt tgc
//
LOCUS       DAZGJW010000706          319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.714, whole genome shotgun sequence.
ACCESSION   DAZGJW010000706 DAZGJW010000000
VERSION     DAZGJW010000706.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.714"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>319
                     /locus_tag="ACHKIK_005944"
     CDS             <1..>319
                     /locus_tag="ACHKIK_005944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086813.1"
                     /translation="AQSITSFHKEKSAMNTGEQIKSFENKRAALAASLEEIMNKAAEE
                     GRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELETSKAATAQPVKQAGNGNVATVASAP
                     VIRV"
BASE COUNT           93 a           78 c           97 g           51 t
ORIGIN      
        1 cggcacagtc aataacctct tttcataagg aaaagtcagc aatgaatact ggtgaacaga
       61 ttaaaagttt tgaaaacaag cgtgcggcgc tggcagcctc ccttgaggag atcatgaaca
      121 aagccgcaga ggaaggccgc acactggatg tggaagagga agagcactac gacaacaccg
      181 cagcggaaat ccgtcaggtg gatgcgcacc tgaagcgtct gcgtgaactg gaaaccagta
      241 aggccgccac ggcacagccg gtgaaacagg ccggtaacgg gaatgtggcc acggtggctt
      301 cagcgccggt gatccgtgt
//
LOCUS       DAZGJW010000707          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.715, whole genome shotgun sequence.
ACCESSION   DAZGJW010000707 DAZGJW010000000
VERSION     DAZGJW010000707.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.715"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>314
                     /locus_tag="ACHKIK_005945"
     CDS             <1..>314
                     /locus_tag="ACHKIK_005945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO9086814.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VY"
BASE COUNT           80 a           70 c           88 g           76 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acat
//
LOCUS       DAZGJW010000708          309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.716, whole genome shotgun sequence.
ACCESSION   DAZGJW010000708 DAZGJW010000000
VERSION     DAZGJW010000708.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.716"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>309
                     /gene="espM1"
                     /locus_tag="ACHKIK_005946"
     CDS             <1..>309
                     /gene="espM1"
                     /locus_tag="ACHKIK_005946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HFO9086815.1"
                     /translation="FSSFGISYHKDNSFRGTIRGKNDEVVKCSMGERSIRFNVNKFSG
                     CILETVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNAKISSEERKMVFSLVS
                     K"
BASE COUNT           96 a           48 c           76 g           89 t
ORIGIN      
        1 ttcagctctt ttggtatcag ttatcataaa gataattctt tccggggcac catccgtggg
       61 aagaatgatg aggtcgtgaa gtgttcaatg ggagagcgct ctatccgctt taatgttaac
      121 aaatttagcg gctgtatact ggagacggta agcaggcaga gtactaaaga tattcatgga
      181 tgggtaagtg atgaacggac agtatatccg tcaagggtta tcaaccaaga gattgataat
      241 tgctgccttc aaaaaaatgc aaaaatttct tctgaagaga ggaaaatggt cttttctctt
      301 gtaagtaag
//
LOCUS       DAZGJW010000709          308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.717, whole genome shotgun sequence.
ACCESSION   DAZGJW010000709 DAZGJW010000000
VERSION     DAZGJW010000709.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.717"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..201)
                     /locus_tag="ACHKIK_005947"
     CDS             complement(<1..201)
                     /locus_tag="ACHKIK_005947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFO9086816.1"
                     /translation="MFSIRSQLPISASVSIPAEQSQSISTIPGGRKIETVPEKEGLLV
                     FLGTKSVGEYALNILGQNVSRVT"
BASE COUNT           84 a           56 c           54 g          114 t
ORIGIN      
        1 tgtgactctt gaaacatttt gaccaagaat attaagagca tattcaccta cggattttgt
       61 tcctaaaaac acaagcaatc cttctttctc tggcactgtc tctatttttc tccctccagg
      121 aattgtggag atggattgag attgttctgc cggaattgat acgctggcgc ttataggtag
      181 ctgagatctt atactaaaca tacaacctcc ttcttagttt ctttaaatgt gagttcatga
      241 taattctaat ggagtgagta atattttagt tgataatagc tgatatcctt ttcagctatt
      301 aatctgtt
//
LOCUS       DAZGJW010000710          304 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.718, whole genome shotgun sequence.
ACCESSION   DAZGJW010000710 DAZGJW010000000
VERSION     DAZGJW010000710.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.718"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>304)
                     /locus_tag="ACHKIK_005948"
     CDS             complement(<1..>304)
                     /locus_tag="ACHKIK_005948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086817.1"
                     /translation="EWINTPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYC
                     LLRAILVLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFNDLREDVKT"
BASE COUNT           90 a           70 c           42 g          102 t
ORIGIN      
        1 aatgtcttta catcttccct gagatcattg aatgccgtat caacaagagc ctcgatcgtt
       61 tgtttatcaa caattttaat aaactcttcg tatacttgct tttgcgtctt atggctgccc
      121 agcgcccatg aatactcatg ttcttttaaa accagtatgg cccttaacag acaatagcca
      181 tcgccatcta ctggagcgta ttttaatcca taagttttat taatttcact ctgaataaaa
      241 gatataccag acaaatcaat accagtatct aaatcagata ttcccggcgt atttatccat
      301 tctg
//
LOCUS       DAZGJW010000711          298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.719, whole genome shotgun sequence.
ACCESSION   DAZGJW010000711 DAZGJW010000000
VERSION     DAZGJW010000711.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.719"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT           99 a           29 c           69 g          101 t
ORIGIN      
        1 aaaaagattg gtgtcacaag tatttattta tgattaatca gagggtttat gacagagaaa
       61 aggatgtata tttctgagta aacatgtaca ggcctatggg gtgactattt aatgagtaaa
      121 tgccgaatga ttttcggggt gatgattata acttgaggaa aaatcccagc gtggtttgag
      181 tagtgtacct gagtgatatt tgtgttatgt atgcattgat tgcagcagaa taaatatagt
      241 tcaggctagt atagtttatg gtagctatga gaatatataa tctattttac ctataaaa
//
LOCUS       DAZGJW010000712          297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.720, whole genome shotgun sequence.
ACCESSION   DAZGJW010000712 DAZGJW010000000
VERSION     DAZGJW010000712.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.720"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>297
                     /locus_tag="ACHKIK_005949"
     CDS             <1..>297
                     /locus_tag="ACHKIK_005949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO9086818.1"
                     /translation="LGIYLYTDAPVPDGVAMTGTGNPFLMSYFTQTTDGRVNLMHHRK
                     AGNTKLGEFGDYGNDWQTLELVFTAGSATVTPKLNGVAGPAFQVIKDSLTLGLNA"
BASE COUNT           68 a           71 c           93 g           65 t
ORIGIN      
        1 ctggggattt atctgtatac ggatgctccc gttcctgatg gtgtggcgat gacgggtacc
       61 ggtaatccgt tcctgatgtc gtacttcact cagaccactg acggcagagt gaatctgatg
      121 catcacagga aagccggaaa cacgaagctg ggggagttcg gcgattacgg taacgactgg
      181 cagacgctgg agctggtgtt caccgccggc agtgccacgg ttactccgaa actgaatgga
      241 gtggctggcc cggcattcca ggttataaaa gacagtctga cactgggact gaatgcg
//
LOCUS       DAZGJW010000713          294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.721, whole genome shotgun sequence.
ACCESSION   DAZGJW010000713 DAZGJW010000000
VERSION     DAZGJW010000713.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.721"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>294)
                     /locus_tag="ACHKIK_005950"
     CDS             complement(<1..>294)
                     /locus_tag="ACHKIK_005950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9086819.1"
                     /translation="QDIAKMRLRLMQTDVQGIRREKRQGDTARLCRRPNAQQNRIQFF
                     ELWLNSKLRHGNTVVLKIRNPRGQIKELRILDWNRVEPLVADDGDVFYRITPDR"
BASE COUNT           61 a           87 c           77 g           69 t
ORIGIN      
        1 cggtccggcg tgatgcggta gaacacgtcg ccgtcatccg ccaccagagg ttcaacccgg
       61 ttccagtcca gaatacgcag ttctttgatc tgcccccggg ggttgcggat tttcagcacc
      121 accgtattgc cgtgacgcaa tttggaattc agccacagtt caaaaaactg gatgcgattc
      181 tgctgcgcat taggacgacg acagagacgg gcagtatctc cctgccgctt ttcccggcgt
      241 attccctgta catcagtctg catcaggcga agccgcattt tggcaatatc ctgg
//
LOCUS       DAZGJW010000714          294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.722, whole genome shotgun sequence.
ACCESSION   DAZGJW010000714 DAZGJW010000000
VERSION     DAZGJW010000714.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.722"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>294
                     /locus_tag="ACHKIK_005951"
     CDS             <1..>294
                     /locus_tag="ACHKIK_005951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9086820.1"
                     /translation="CDAIVQAPAPSRPIERGIAGPGLLARVLISKYAEHTPLYRQSEM
                     YGRQGVELSRSLLSGWVDACCRLLSPLEEALQDYVLTDGKLHADDTPVPVLLPG"
BASE COUNT           48 a           90 c           98 g           58 t
ORIGIN      
        1 gtgcgatgcc atcgtgcagg cccccgcgcc ttcacggccc atcgagcggg gtatcgcagg
       61 accggggctg ctggcccgcg tgctgatctc aaagtatgca gagcacaccc cgctgtaccg
      121 ccagtctgaa atgtacggcc gccagggcgt ggagctgagt cgttcactgc tgtcgggctg
      181 ggtggatgca tgctgccggc tactgtcacc gctggaagaa gcgcttcagg actatgtgct
      241 gactgacggt aagctccatg ctgatgacac gcctgtcccg gtgctgttgc cagg
//
LOCUS       DAZGJW010000715          292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.723, whole genome shotgun sequence.
ACCESSION   DAZGJW010000715 DAZGJW010000000
VERSION     DAZGJW010000715.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.723"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>292)
                     /locus_tag="ACHKIK_005952"
     CDS             complement(<1..>292)
                     /locus_tag="ACHKIK_005952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086821.1"
                     /translation="SFFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPK
                     ARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTAD"
BASE COUNT           66 a           90 c           79 g           57 t
ORIGIN      
        1 cgtctgccgt gctgatctcc tgagaaacca cgcgagaccc cacgcgcatt tccccgtaca
       61 gaacaggcag aacattgccc tgggcaacca tgttatccag tgaggagaaa taggtgttct
      121 gcttaccgtt atccgttgtc tgtgtacggg gagttctggc tttcggtgcc agcatctgcg
      181 ccacaccacc gagcaccata ctggcaccga gagaaaacag gatgccggtc ataccaccgg
      241 ccccaatggc tgccccccat gctgcaaggg tggctccggc ggtaaagaat ga
//
LOCUS       DAZGJW010000716          290 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.724, whole genome shotgun sequence.
ACCESSION   DAZGJW010000716 DAZGJW010000000
VERSION     DAZGJW010000716.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.724"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            15..>290
                     /locus_tag="ACHKIK_005953"
     CDS             15..>290
                     /locus_tag="ACHKIK_005953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086822.1"
                     /translation="MNIQPIVTSGITTQNNRHHHAEQTSPTQIPQSELPNGCETGFVV
                     HIPEDMQRHAPECGETTALLSLIKDEGLLSGLDKYLAPHLEEGSAGKK"
BASE COUNT          102 a           69 c           57 g           62 t
ORIGIN      
        1 aataaggatt tatcatgaac attcaaccga tcgtaacatc cggaatcacc acacaaaaca
       61 atcgacatca tcacgcagaa caaacgtccc ctacacaaat accgcaatcc gaattaccta
      121 atggatgcga aacgggattt gttgttcata tcccagagga tatgcagcga catgcaccgg
      181 aatgcggtga aacaacagct ctactgagct tgataaaaga tgaaggtctg ctctctgggc
      241 tggataaata tcttgcacct catcttgaag aaggctctgc aggaaaaaaa
//
LOCUS       DAZGJW010000717          290 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.725, whole genome shotgun sequence.
ACCESSION   DAZGJW010000717 DAZGJW010000000
VERSION     DAZGJW010000717.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.725"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>290
                     /locus_tag="ACHKIK_005954"
     CDS             <1..>290
                     /locus_tag="ACHKIK_005954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFO9086823.1"
                     /translation="LFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSR
                     KGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSR"
BASE COUNT           78 a           73 c           71 g           68 t
ORIGIN      
        1 ctgtttatgg cgctcaggag ccagcgccca cctgccgggc taatccacca ctctgatcga
       61 ggttcacagt actgcgcata cgattaccgg gtcatacagg agcagtttgg tctgaaaaca
      121 tcaatgtcgc gtaaaggtaa ctgttacgac aacgctccga tggaaagctt ctggggaacg
      181 ctgaaaaatg agagcctgag ccactatcgt tttaataacc gggatgaagc catctcagta
      241 atacgggaat acattgagat tttctacaat cgtcagcgtc gtcactctcg
//
LOCUS       DAZGJW010000718          290 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.726, whole genome shotgun sequence.
ACCESSION   DAZGJW010000718 DAZGJW010000000
VERSION     DAZGJW010000718.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.726"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..240
                     /locus_tag="ACHKIK_005955"
     CDS             <1..240
                     /locus_tag="ACHKIK_005955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFO9086824.1"
                     /translation="VSEVLDKFRKNYDRIMSLPQETKEEKEFRHCIWLAEKEERERIY
                     QTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
BASE COUNT           92 a           71 c           66 g           61 t
ORIGIN      
        1 gtatcggagg tgctggataa attcagaaaa aactacgatc ggataatgtc gctaccgcag
       61 gaaacgaaag aggaaaagga atttcgccat tgtatatggc ttgcagagaa agaagaacgc
      121 gagcgaattt accagacatc aatccgacca ttccgcaaag ccacatatac ccacttccct
      181 gaaattgacc cgcgcctgcg taattaccgc tcacgctatg gcgctatcag taatgactga
      241 ggaatttacc atgagaggac ttgcatacaa tcccggcatt cttccggcag
//
LOCUS       DAZGJW010000719          290 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.727, whole genome shotgun sequence.
ACCESSION   DAZGJW010000719 DAZGJW010000000
VERSION     DAZGJW010000719.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.727"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            188..>290
                     /locus_tag="ACHKIK_005956"
     CDS             188..>290
                     /locus_tag="ACHKIK_005956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFO9086825.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVF"
BASE COUNT           78 a           49 c           66 g           97 t
ORIGIN      
        1 ttttttcttt agttcatgta tttcgatttg tttctgaatt atcgggggta gtgtcttccc
       61 ttttccctga ggcttatcat gcagttattt tcgccatctt ggtattgtgg aaataccgac
      121 atccatagct ttggtgagct ctgttcaggt ggccaaattc agaaaaccat tacggaggaa
      181 gaaggcgatg gctaaaccag cgcgaagacg atgtaaccgt aaaagagaag atttaactgt
      241 taaaaggata tttgagttac taagtttcga taaatctacc ggggtattta
//
LOCUS       DAZGJW010000720          287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.728, whole genome shotgun sequence.
ACCESSION   DAZGJW010000720 DAZGJW010000000
VERSION     DAZGJW010000720.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.728"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>287
                     /locus_tag="ACHKIK_005957"
     rRNA            <1..>287
                     /locus_tag="ACHKIK_005957"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           87 a           67 c           80 g           53 t
ORIGIN      
        1 cctgcttcca ggaaaagcct ctaagcatca ggtaacatca aatcgtaccc caaaccgaca
       61 caggtggtca ggtagagaat accaaggcgc ttgagagaac tcgggtgaag gaactaggca
      121 aaatggtgcc gtaacttcgg gagaaggcac gctgatatgt aggtgaagtc cctcgcggat
      181 ggagctgaaa tcagtcgaag ataccagctg gctgcaactg tttattaaaa acacagcact
      241 gtgcaaacac gaaagtggac gtatacggtg tgacgcctgc ccggtgc
//
LOCUS       DAZGJW010000721          285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.729, whole genome shotgun sequence.
ACCESSION   DAZGJW010000721 DAZGJW010000000
VERSION     DAZGJW010000721.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.729"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT           88 a           49 c           67 g           81 t
ORIGIN      
        1 tgctgatgct gacggatgtt acaaaaaatg cagcgtatgg cgttgagata gaaagtctgg
       61 tgctggagat aaatacacct acatcatgat aaaaaagggc cagcgactga cctgaagaag
      121 acactggcta aaaggctttg catgtttgta gatacttatt tttcacagac agcaatgatg
      181 cctgtcaata tattattaat atgctgattg tttcagttac agatgcttta ttaagggaaa
      241 atgtcacgat cggtgcaaaa gtggtttgaa ccgccccggt tttcc
//
LOCUS       DAZGJW010000722          284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.730, whole genome shotgun sequence.
ACCESSION   DAZGJW010000722 DAZGJW010000000
VERSION     DAZGJW010000722.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.730"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>284
                     /locus_tag="ACHKIK_005958"
     CDS             <1..>284
                     /locus_tag="ACHKIK_005958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure C-terminal
                     domain-containing protein"
                     /protein_id="HFO9086826.1"
                     /translation="ADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNP
                     LALNNVMSEQKKTWAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAA"
BASE COUNT           81 a           67 c          101 g           35 t
ORIGIN      
        1 aggcggataa attcgcacag cagcaacggg caaaacgggc cgccattgat gcgaaaagcc
       61 gggggctgac tgaccggcag gcagaacggg aagccacgga acagcgcctg aaggaacagt
      121 atggcgataa tccgctggcg ctgaataacg tcatgtcaga gcagaaaaag acctgggcgg
      181 ctgaagacca gcttcgcggg agctggatgg caggcctgaa gtccggctgg agtgagtggg
      241 aagagagcgc cacggacagt atgtcgcagg taaaaagtgc agcc
//
LOCUS       DAZGJW010000723          283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.731, whole genome shotgun sequence.
ACCESSION   DAZGJW010000723 DAZGJW010000000
VERSION     DAZGJW010000723.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.731"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT           84 a           58 c           67 g           74 t
ORIGIN      
        1 cttctcctgc aagagaatta ttttaatttt cgcttaattc agcgaaacca aagtaaagtg
       61 gtcaagcccc gcgcataggg caagcttagg cagaagaaag gactaaagcg cgttgcgcca
      121 gtgctgctca cctacgagca agatagtgga tgaggtacga agatgggtaa atgttgaaaa
      181 taacgtcctg aacaaattgt ccataatatt acaattatcc gcagtgttgc ttctcgtcat
      241 cgcggatgat aattgtcctc ggtagttgaa cacgcctgat ttg
//
LOCUS       DAZGJW010000724          281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.732, whole genome shotgun sequence.
ACCESSION   DAZGJW010000724 DAZGJW010000000
VERSION     DAZGJW010000724.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.732"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..279)
                     /gene="tccP2"
                     /locus_tag="ACHKIK_005959"
     CDS             complement(<1..279)
                     /gene="tccP2"
                     /locus_tag="ACHKIK_005959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
                     /protein_id="HFO9086827.1"
                     /translation="MNSINSFFSSIPHSISSVTRNSSFTASQHKSTPNTVKTSSPLSP
                     SNSPASATTIFKVKNSYTESGLQRSTSYTQSSIEKNALHRPLPDVAKRL"
BASE COUNT           75 a           39 c           76 g           91 t
ORIGIN      
        1 taggcgtttg gccacatcag gtagaggccg atgtaaagcg tttttttcta tacttgactg
       61 agtataagat gtggaacgtt gaagtcctga ttccgtataa gaatttttta ccttaaaaat
      121 agttgttgca ctggcaggac tgttgctggg agaaagaggt gagctggttt ttaccgtgtt
      181 aggtgtgctt ttgtgctgtg aggcagtgaa gctactgttg cgcgtaacac ttgatatact
      241 gtgaggaatg ctggaaaaaa aagaattaat gctatttatc a
//
LOCUS       DAZGJW010000725          273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.denovo.733, whole genome shotgun sequence.
ACCESSION   DAZGJW010000725 DAZGJW010000000
VERSION     DAZGJW010000725.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.denovo.733"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            19..95
                     /locus_tag="ACHKIK_005960"
     tRNA            19..95
                     /locus_tag="ACHKIK_005960"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53..55,aa:Ile,seq:gat)
     gene            138..213
                     /locus_tag="ACHKIK_005961"
     tRNA            138..213
                     /locus_tag="ACHKIK_005961"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:171..173,aa:Ala,seq:tgc)
BASE COUNT           71 a           62 c           73 g           67 t
ORIGIN      
        1 gagcagtaaa acctctacag gcttgtagct caggtggtta gagcgcaccc ctgataaggg
       61 tgaggtcggt ggttcaagtc cactcaggcc taccaaattt gcacggcaaa tttgaagagg
      121 ttttaactac atgttatggg gctatagctc agctgggaga gcgcctgctt tgcacgcagg
      181 aggtctgcgg ttcgatcccg catagctcca ccatctctgt agtgattaaa taaaaaatac
      241 ttcagagtgt acctgcaaag gttcactgcg aag
//
LOCUS       DAZGJW010000726         6529 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.guided.214, whole genome shotgun sequence.
ACCESSION   DAZGJW010000726 DAZGJW010000000
VERSION     DAZGJW010000726.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.guided.214"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..986
                     /locus_tag="ACHKIK_005962"
     CDS             <1..986
                     /locus_tag="ACHKIK_005962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO9086828.1"
                     /translation="GYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKF
                     EKRLSDLDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFA
                     LAGLDAQHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFA
                     CINRQMIALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIP
                     GSRGDYSWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVI
                     CRDKQLIFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            983..1597
                     /gene="prfH"
                     /locus_tag="ACHKIK_005963"
     CDS             983..1597
                     /gene="prfH"
                     /locus_tag="ACHKIK_005963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO9086829.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSLRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1654..3111)
                     /gene="pepD"
                     /locus_tag="ACHKIK_005964"
     CDS             complement(1654..3111)
                     /gene="pepD"
                     /locus_tag="ACHKIK_005964"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO9086830.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3372..3830
                     /gene="gpt"
                     /locus_tag="ACHKIK_005965"
     CDS             3372..3830
                     /gene="gpt"
                     /locus_tag="ACHKIK_005965"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO9086831.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3922..5166
                     /gene="frsA"
                     /locus_tag="ACHKIK_005966"
     CDS             3922..5166
                     /gene="frsA"
                     /locus_tag="ACHKIK_005966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO9086832.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5224..5625
                     /gene="crl"
                     /locus_tag="ACHKIK_005967"
     CDS             5224..5625
                     /gene="crl"
                     /locus_tag="ACHKIK_005967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO9086833.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5664..>6529)
                     /gene="phoE"
                     /locus_tag="ACHKIK_005968"
     CDS             complement(5664..>6529)
                     /gene="phoE"
                     /locus_tag="ACHKIK_005968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO9086834.1"
                     /translation="TQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLKYKDLG
                     SFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGVIDGL
                     NLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQSRGT
                     GKRAEAWATGLKYDANNFYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDFGLRP
                     SLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINND
                     DIVAVGMTYQF"
BASE COUNT         1570 a         1604 c         1716 g         1639 t
ORIGIN      
        1 gcggctatcc gattggcgca gcgttcttct ctgttggtcg tttttatccg gcactggtcg
       61 gcaatgatat cggctgcggt atggcgctat ggcaaacaga tattctcgct cgcaaataca
      121 acgccgataa gtttgaaaag cgattatcag atctggatga cgttgctgaa gaaagctggc
      181 tggaggaaaa cctgccgtca gcatttgcac agcatccgtg gcgcagctcg cttggctcta
      241 tcggtggcgg caatcatttc gctgaactgc aacaagttga tcaaatcatc aacgctgaac
      301 tgtttgcact ggcaggtctg gatgcacaac atctgcaact gttggttcat agcggctcgc
      361 ggggggtggg gcagtctatt ttacagcggc atattgcctc gttctcgcat catggtttgc
      421 ctcaaggcag tgacgacgcg ctacggtata ttgcggaaca tgacgatgcg ctggcgtttg
      481 cgtgtattaa tcgccagatg atcgctttgc gcataatgca acaagttaag gccacgggaa
      541 gtccggttct ggatgtggcg cataactttg ttagcgcgtg tcaaatcggc gatcaacagg
      601 gctggttgca tcgtaaaggt gccacgccgg atgacaacgg tctggtgatt attcccggtt
      661 cacgtggtga ttactcctgg attgtacagc ctgttgcgaa cgaaaaaacg ctccactcgc
      721 tggcgcatgg ggcagggcgt aaatgggggc gcaccgagtg taaagggcgt ctggcagcga
      781 aatacacggc gacgcaactc tcgcgtactg aacttggcag tcgggtaatt tgtcgcgata
      841 aacaactcat ctttgaagaa gcgccacaag cctataaatc ggctgaaagc gtggtgcaat
      901 gtctggtgca ggccggttta attattcctg tcgcgcggct gcgtccggtg ctaacgctca
      961 aaaacagtgg agggaaaaaa ggatgatctt gctacaactc tcctcagcgc aggggccgga
     1021 agaatgttgt ctcgcagtga aaaaggcgct ggacaggcta attaaagaag ctgcccgaca
     1081 agacgtcgcg gtaacggtgc tggaaacaga aacgggccac tactctgaca cgctgcgttc
     1141 ggcgctgatt tctctggatg gcgataacgc atgggcatta agcgaaagct ggtgtggcac
     1201 tattcagtgg atttgtccga gtccgtatcg gcctcatcat gggcgcaaaa actggtttct
     1261 gggcattggg cgttttaccg ctgatgagca ggaacaatcg gatgcaatcc gttatgagac
     1321 gttgcgttcg tcggggccgg gcggtcaaca tgtcaataaa accgactcgg cggtacgcgc
     1381 cacgcatttg gcatccggta ttagcgtgaa ggttcagtca gagcgtagtc agcatgctaa
     1441 caagcggctg gcacgattgc tgattgcctg gaagctggag cagcagcaac aggaaaatag
     1501 cgcggtgctg aaatcgctgc ggcgaatgtt ccatcaccag attgaacgtg gcaacccgag
     1561 acggacattt acagggatgg cttttatcga aggataatga aggatgaaac tgccggaagg
     1621 cgattaaacg ccatccggca gcaaatcaaa taattacttc gccggaattt ctttcagcag
     1681 ttcagtcagc agcgtccagt aatgacctac gctttcgatg tgaacttgct catccggaga
     1741 gtgtggaccg gtgatagttg gcccgataga aaccatgtcc atttccggat acggtttttt
     1801 gaacagacca cattccaggc ccgcgtggat aatctggatg ttcggtgttt tgttgaacag
     1861 acgctgatag gtttcacgta ccagatgcat caccggagaa ttagcgtccg gctgccagcc
     1921 aggatatgcg cctttcgctt cggttttcgc gccagctagt ttacccagcg aatccagcat
     1981 gctcaccacg tagtctttac cgctgtcgat cagtgaacgg atcaggcagt gaatttctac
     2041 gttattgtca gtcatggtca ccacaccgac gttcagggag gtttcgacca caccttttgc
     2101 cacgtcggaa ttgcggatca caccgttcgg ggtggcgttc agcagacgaa taaaggtatc
     2161 gcgagatttc gcaatcaggg cagctttatc gttcgctaca gagtccagca acaaggccag
     2221 atttttctct ttctctgcca gctcgttttt caggatctcc tgataggtat tcaccagaga
     2281 tttcaggacg tcgactttat cagctgcgac agcaatggtc gcaaaggctt cacgcgggat
     2341 ggcgttacgc agtgtgccgc cgttgaaatc gataaggcgc agatccagtt cttccgcatg
     2401 acccgccagg aagcgcacca gtagtttgtt ggcattaccc aggccaacgt ggatttcacc
     2461 gccggagtga ccgcctttca gaccttttaa ggttaacttg aaggtttcaa aaccagctgg
     2521 aaccgcttca cgatctaaat gcaggttgga ggtgaagtcg ataccccccg cacaacccat
     2581 gtagatttca ccttcttctt cggagtcggt gttaatcaga atatcagcct gcaaccagtt
     2641 gctctgtaag ccgaacgcac cgtccatacc ggcttcttcg gtcatggtca gcagcacttc
     2701 cagcgggccg tgaaccacgt tttcgtcagc cagaaccgcc agcgcagacg ccataccaat
     2761 gccgttatct gcacccagcg tggtgccgcg cgctttaacc cattcgccat caatataagg
     2821 ctggatagga tctttcgtga agtcatgcac ggtgtcgtta tttttctgcg gcaccatatc
     2881 gaggtgggcc tgtaagacga ccggtttacg attttccata cctgcggtag caggtttacg
     2941 aatcaggata ttacctacct gatcgcgttc gacatggaaa cctttctctt ttgcccaacc
     3001 aacaatgtat tcagcgagtt gctcttcatg ataggacggg tgaggaatag aacagatttt
     3061 ggcaaaaata tcccacagcg gctgtggaga taattgagac agttcagaca cgttaagtct
     3121 ccttgtcgat cacccgcaaa acagtattgc aggtcacagg gttagcagaa aatgttgtca
     3181 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
     3241 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
     3301 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
     3361 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
     3421 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
     3481 gtggcggtct ggtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata
     3541 ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg
     3601 cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg
     3661 ctgttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg
     3721 ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc tggatcgaac
     3781 agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac
     3841 gcctggcact gccgggcgtt gttcttttta acttcaggca ggttacaata gtttccagta
     3901 agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc
     3961 cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg
     4021 cctgtgcagt cggctcttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt
     4081 ctgatgtgga tctggcgcgg tattgaccca cgtgaaatcc tcgacgtcca ggcacgtatt
     4141 gtgatgagcg atgctgaacg taccgacgat gatttatacg atactgtgat tggctaccgt
     4201 ggtggcaact ggatttatga gtgggccacc caggcgatgg tgtggcaaca aaaagcctgt
     4261 gcggaagaag atccgcaact cagtggtcga cactggctgc atgcggctac gttgtacaac
     4321 attgccgcct atcctcatct gaaaggagat gacctggccg agcaagcgca ggctttatca
     4381 aaccgcgcct atgaagaggc cgcccagcgt ctgccgggca cgatgcggca gatggagttt
     4441 accgtacccg gcggtgcgcc catcaccggc tttttgcata tgccgaaagg cgatggcccg
     4501 ttcccgacag tattaatgtg tggtggtctg gatgcgatgc agacggacta ttacagcctg
     4561 tatgaacgtt attttgcgcc gcgcggcatt gcgatgctga ctattgatat gccatcggtg
     4621 ggcttttctt caaaatggaa gctcacccag gactccagcc tgttgcatca gcacgtctta
     4681 aaggcgctgc ctaacgtacc gtgggtggat cacactcgcg tcgcggcctt tggtttccgt
     4741 ttcggcgcta acgttgccgt gcgtctggca tatcttgaat cgccgcgtct gaaagcggtt
     4801 gcctgtcttg gtccggtagt tcataccctg ttgagtgatt ttaagtgcca gcaacaggtg
     4861 ccggaaatgt atcttgacgt tctggcgagt cgtttgggga tgcatgatgc ttccgatgac
     4921 gcgttacgcg tggagctgaa tcgctattca ttaaaagtgc aaggattgct ggggcgtcgc
     4981 tgcccaacgc caatgttatc aggctactgg aagaacgatc cgttcagccc ggaagaggac
     5041 tcacgcttaa tcacctcatc atctgctgac ggtaaattat tagagatccc atttaacccg
     5101 gtgtatcgga attttgacaa aggtcttcag gaaatcaccg gctggatcga aaaacgcttg
     5161 tgttaaaaat ttgctaaatt ttgccaattt ggtaaaacag ttgcatcaca acaggagata
     5221 gcaatgacgt taccgagtgg acacccgaag agcagattga tcaaaaaatt taccgcacta
     5281 ggcccgtata ttcgtgaagg taagtgcgaa gataatcgat tctttttcga ttgtctggct
     5341 gtatgcgtca acgtgaaacc ggcaccggaa gtgcgtgagt tctggggctg gtggatggag
     5401 cttgaagcgc aggaatcccg ttttacctac agttaccagt ttggtctgtt cgataaagca
     5461 ggcgactgga agagtgttcc ggtaaaagac actgaagtgg ttgaacgact ggagcacacc
     5521 ctgcgtgagt ttcacgagaa gctgcgtgaa ctgctgacga cgctgaatct gaagctggaa
     5581 ccggcggatg attttcgtga cgagccggtg aagttaacgg cgtgagtgaa atgtgccgga
     5641 tgcatcacat ccggcaataa tcattaaaac tgataggtca tgccaaccgc gacaatatca
     5701 tcattattaa tattcaattt gttatcgcta tccagttggt tgattttata atcaacaaac
     5761 gctgacatat ttttgttgaa ataatatgta gccccgacat cgatataatt gaccagatct
     5821 tcatcaccga taccttcaat atctttccct ttcgataaga cataacccag cgatggacgc
     5881 agaccaaagt caaactggta ttgagcgacc gcttcaaagt tctgtgtctt attggcaaag
     5941 ccgccagtta ttggcgtcat tttgcgtgtt tcagaataaa aagttgccag ataaaaatta
     6001 ttggcatcgt atttcagacc tgtagcccat gcttctgcac gcttgcctgt gccacggctt
     6061 tgcaggttct gctcgttggt gcgatctgag ttggtatagg ccccactaat ggcgaaatcg
     6121 ctgccgccaa agtcatatgt caatgacgtg ccgaagccat cgccgttttg ctttttaacg
     6181 tcgcggtttt cgtttttccc ttgatattgc agggttaagt tcaggccatc gataacgccg
     6241 aagaagtcgg tgttccgata cgtcgccaga ccgctggcgc gtttggtcat aaagttgtcg
     6301 gtctgcgccg aggaatcgcc accaaattcc gggaacatat cggtccaggc ttccacgtca
     6361 tacaacgcgc ccaggttacg accataatcg aaagaaccca aatctttata tttcaacccg
     6421 gcaaaagcga gacgcgtttt ttgctgtgcg gtatcactct ccgctttatt tccggcaaac
     6481 tccgcttccc accgaccata accagtcagt tgatcgttaa tttgtgttt
//
LOCUS       DAZGJW010000727         6163 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.guided.220, whole genome shotgun sequence.
ACCESSION   DAZGJW010000727 DAZGJW010000000
VERSION     DAZGJW010000727.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.guided.220"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            213..2021
                     /gene="frdA"
                     /locus_tag="ACHKIK_005969"
     CDS             213..2021
                     /gene="frdA"
                     /locus_tag="ACHKIK_005969"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO9086835.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            2014..2748
                     /gene="frdB"
                     /locus_tag="ACHKIK_005970"
     CDS             2014..2748
                     /gene="frdB"
                     /locus_tag="ACHKIK_005970"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO9086836.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2759..3154
                     /gene="frdC"
                     /locus_tag="ACHKIK_005971"
     CDS             2759..3154
                     /gene="frdC"
                     /locus_tag="ACHKIK_005971"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO9086837.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3165..3524
                     /gene="frdD"
                     /locus_tag="ACHKIK_005972"
     CDS             3165..3524
                     /gene="frdD"
                     /locus_tag="ACHKIK_005972"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO9086838.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            3587..4720
                     /gene="ampC"
                     /locus_tag="ACHKIK_005973"
     CDS             3587..4720
                     /gene="ampC"
                     /locus_tag="ACHKIK_005973"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO9086839.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            4809..5342
                     /gene="blc"
                     /locus_tag="ACHKIK_005974"
     CDS             4809..5342
                     /gene="blc"
                     /locus_tag="ACHKIK_005974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO9086840.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(5339..5656)
                     /gene="sugE"
                     /locus_tag="ACHKIK_005975"
     CDS             complement(5339..5656)
                     /gene="sugE"
                     /locus_tag="ACHKIK_005975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO9086841.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(5838..5984)
                     /gene="ecnB"
                     /locus_tag="ACHKIK_005976"
     CDS             complement(5838..5984)
                     /gene="ecnB"
                     /locus_tag="ACHKIK_005976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO9086842.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(6095..>6163)
                     /locus_tag="ACHKIK_005977"
     CDS             complement(6095..>6163)
                     /locus_tag="ACHKIK_005977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HFO9086843.1"
                     /translation="NTARGFGEDIKHLGNSISRAAS"
BASE COUNT         1530 a         1590 c         1634 g         1409 t
ORIGIN      
        1 ctgatggttt agtaattaaa ttaatcatct tcagtgataa tttagccctc ttgcgcacta
       61 aaaaaatcga tctcgtcaaa tttcagactt atccatcaga ctatactgtt gtacctataa
      121 aggagcagtg gaatagcgtt cgcagaccgt aactttcagg cacttaccct gaagtacggg
      181 gctgtgggat aaaaacaatc tggaggaatg tcgtgcaaac ctttcaagcc gatcttgcca
      241 ttgtaggcgc cggtggcgcg ggattacgtg ctgcaattgc tgccgcgcag gcaaatccaa
      301 atgcaaaaat cgcactaatc tcaaaagtat acccgatgcg tagccatacc gttgctgcag
      361 aagggggctc cgccgctgtc gcgcaggatc atgacagctt cgaatatcac tttcacgata
      421 cagtagcggg tggcgactgg ttgtgtgagc aggatgtcgt ggattatttc gtccaccact
      481 gcccaaccga aatgacccaa ctggaactgt gggggtgccc atggagccgt cgcccggatg
      541 gtagcgtcaa cgtacgtcgc ttcggcggca tgaaaatcga gcgcacctgg ttcgccgccg
      601 ataagaccgg cttccatatg ctgcacacgc tgttccagac ctctctgcaa ttcccgcaga
      661 tccagcgttt tgacgaacat ttcgtgctgg atattctggt tgatgatggt catgttcgcg
      721 gcgtggtagc aatgaacatg atggaaggca cgctggtgca gatccgtgct aacgcggtcg
      781 ttatggctac tggcggtgcg ggtcgcgttt atcgttacaa caccaacggc ggcatcgtta
      841 ccggtgacgg tatgggtatg gcgctaagcc acggcgttcc gctgcgtgac atggaattcg
      901 ttcagtatca cccaaccggt ctgccaggtt ccggtatcct gatgaccgaa ggctgccgcg
      961 gtgaaggcgg tattctggtc aacaaaaatg gctaccgtta tctgcaagat tacggcatgg
     1021 gcccggaaac tccgctgggc gagccgaaaa acaaatatat ggaactgggt ccacgcgaca
     1081 aagtttctca ggccttctgg cacgaatggc gtaaaggcaa caccatctcc acgccgcgtg
     1141 gcgatgtggt ttatctcgac ctgcgtcacc tcggcgagaa aaaactgcat gaacgtctgc
     1201 cgttcatctg cgaactggcg aaagcgtacg ttggcgtcga tccggttaaa gaaccgattc
     1261 cggtacgtcc gaccgcacac tacaccatgg gcggtatcga aaccgatcag aactgtgaaa
     1321 cccgcattaa aggtctgttc gccgtgggtg aatgttcctc tgttggtctg cacggtgcaa
     1381 accgtctggg ttctaactcc ctggcggaac tggtggtctt cggccgtctg gccggtgaac
     1441 aagcgacaga gcgtgcagca actgccggta atggcaacga agcggcaatt gaagcgcagg
     1501 cagctggcgt tgaacaacgt ctgaaagatc tggttaacca ggatggcggc gaaaactggg
     1561 cgaagatccg cgacgaaatg ggcctggcaa tggaagaagg ttgcggtatc taccgtacgc
     1621 cggaactgat gcagaaaacc atcgacaagc tggcagagct gcaggaacgc ttcaagcgcg
     1681 tgcgcatcac cgacacctcc agcgtgttca acaccgacct gctctacacc attgaactgg
     1741 gccacggtct gaacgttgct gaatgtatgg cgcactccgc aatggcacgt aaagagtccc
     1801 gcggcgcaca ccagcgtctg gacgaaggtt gcaccgagcg tgacgacgtc aacttcctca
     1861 aacacaccct cgccttccgc gatgctgatg gcacgactcg cctggagtac agcgacgtga
     1921 agattactac gctgccgcca gctaaacgcg tttacggtgg cgaagcggat gcagccgata
     1981 aggcggaagc agccaataag aaggagaagg cgaatggctg agatgaaaaa cctgaaaatt
     2041 gaggtggtgc gctataaccc ggaagtcgat accgcaccgc atagcgcatt ctatgaagtg
     2101 ccttatgacg caactacctc attactggat gcgctgggct acatcaaaga caacctggca
     2161 ccggacctga gttaccgctg gtcctgccgt atggcgattt gtggttcctg cggcatgatg
     2221 gttaacaacg tgccaaaact ggcatgtaaa accttcctgc gtgattacac cgacggtatg
     2281 aaggttgaag cgttagctaa cttcccgatt gaacgcgatc tggtggtcga tatgacccac
     2341 ttcatcgaaa gtctggaagc gatcaaaccg tacatcatcg gcaactcccg caccgcggat
     2401 cagggtacta acatccagac cccggcgcag atggcaaagt atcaccagtt ctccggttgc
     2461 atcaactgtg gtctgtgcta cgccgcgtgc ccgcagtttg gcctgaaccc agagttcatc
     2521 ggtccggctg ccattacgct ggcgcatcgt tataacgaag atagccgcga ccacggtaag
     2581 aaggagcgta tggcgcagtt gaacagccag aacggcgtat ggagctgtac tttcgtgggc
     2641 tattgctccg aagtctgccc gaaacacgtc gatccggctg cggccattca gcagggcaaa
     2701 gtagaaagtt cgaaagactt tcttatcgcg accctgaaac cacgctaagg agtgcaacat
     2761 gacgactaaa cgtaaaccgt atgtacggcc aatgacgtcc acctggtgga aaaaattgcc
     2821 gttttatcgc ttttacatgc tgcgcgaagg cacggcggtt ccggctgtgt ggttcagcat
     2881 tgaactgatt ttcgggctgt ttgccctgaa aaatggcccg gaagcctggg cgggattcgt
     2941 cgacttttta caaaacccgg ttatcgtgat cattaacctg atcactctgg cggcagctct
     3001 gctgcacacc aaaacctggt ttgaactggc accgaaagcg gccaatatca ttgtaaaaga
     3061 cgaaaaaatg ggaccagagc caattatcaa aagtctctgg gcggtaactg tggttgccac
     3121 catcgtaatc ctgtttgttg ccctgtactg gtaaggagcc tgagatgatt aatccaaatc
     3181 caaagcgttc tgacgaaccg gtattctggg gcctcttcgg ggccggtggt atgtggagcg
     3241 caattattgc gccggtgatg atcctgctgg tgggtattct gctgccactg gggctgtttc
     3301 cgggtgatgc gctgagctac gagcgcgttc tggcgttcgc gcagagcttc attggtcgcg
     3361 tattcctgtt cctgatgatc gttctgccgc tgtggtgtgg tttacaccgt atgcaccacg
     3421 cgatgcacga tctgaaaatt cacgtgcctg cgggcaaatg ggttttctac ggtctggctg
     3481 ctatcctgac agttgtcacg ctgattggta tcgttacaat ctaacgtatc gccaatgtaa
     3541 atccggcccg cctatggcgg gccgttttgt atggaaacca gaccttatgt tcaaaacgac
     3601 gctctgcgcc ttattaatta ccgcctcttg ctccacattt gctgcccctc aacaaatcaa
     3661 cgatattgtg catcgcacaa ttaccccgct tatagagcaa caaaagatcc ccggtatggc
     3721 ggtggcggta atttatcagg gtaaacctta ttactttacc tggggctatg cggacatcgc
     3781 caaaaagcag cccgtcacac agcaaacgtt gtttgagtta ggttcggtca gcaaaacatt
     3841 tacgggcgtg cttggtggcg acgctattgc tcgaggggaa atcaagttaa gcgatcccac
     3901 aacaaaatac tggcctgaac ttaccgctaa acagtggaat gggatcacac tattacatct
     3961 cgcaacctac actgctggcg gcctgccatt gcaggtgccg gatgaggtga aatcctcaag
     4021 cgacttgctg cgcttctatc aaaactggca gcctgcatgg gcgccaggaa cacaacgtct
     4081 gtatgccaac tccagtatcg gtttgttcgg cgcactggct gtgaagccgt ctggtttgag
     4141 ttttgagcag gcgatgcaaa cacgtgtctt ccagccactc aaactcaacc atacgtggat
     4201 taatgtaccg cccgcagaag aaaagaatta cgcctgggga tatcgcgaag gcaaggcagt
     4261 tcatgtttcg ccaggggcgt tagatgctga aacttatggt gtgaagtcga ccattgaaga
     4321 tatggcctgc tgggtacgaa gcaatatgaa tccccgtgat atcaacgaca aaacacttca
     4381 gcaagggata caactggcac aatctcgcta ctggcaaacc ggcgatatgt atcagggcct
     4441 gggctgggaa atgctggact ggccggtaaa tcctgacagc atcattaacg gcagtggcaa
     4501 taaaattgca ctggcagcac accccgtaaa agcgattacg cccccaactc ctgcagtacg
     4561 cgcatcatgg gtacataaaa caggggcaac cggcggattt ggtagctatg tcgcgtttat
     4621 tccagaaaaa gagctgggta tagtgatgct ggctaacaaa aactatccca atccagcgag
     4681 agtcgccgcc gcctggcaga ttctcaacgc tctacagtaa aatttcatcg ggtccgaatt
     4741 ttcggacctt ttctgcgctt ttccttgctg tcatctacac ttagaaaaaa accagtaagg
     4801 aaacatttat gcgcctgctc cctctcgttg ccgcagcgac agctgcattt ctggtcgttg
     4861 cctgcagttc tcctacgccg ccgcgtggcg taacagtagt gaataatttc gacgccaaac
     4921 gttatctcgg tacctggtat gagattgccc gttttgatca ccgttttgaa cgtggactgg
     4981 aaaaagtcac cgcaacatac agcctgcgtg atgacggcgg cctgaatgtc attaataaag
     5041 gctataaccc tgacagagga atgtggcagc agagtgaagg gaaagcgtac tttaccggcg
     5101 caccaactcg cgctgcgctg aaagtgtcat tctttggtcc tttctatggc ggttataacg
     5161 ttattgcact cgatcgggaa taccgccatg cgctggtttg cggcccggac cgcgactacc
     5221 tgtggatact ctcccgcacg ccaaccattt ctgacgaagt gaaacaggag atgctggcag
     5281 tcgcgacccg ggaagggttt gatgtcagta agtttatttg ggtacagcag cccggtagtt
     5341 agtgagtgct gagtttcaga ccaataatcc ccaatacgat taacgccaga ctcgccaggc
     5401 gcatcgggtt agcggactca ccgagcagca caatgccggt gatggccgcg ccgactgcgc
     5461 caatacccgt ccacacggca taagccgtcc ctactggtaa cgatttcatc gcccaggcaa
     5521 gtagcgccat actgacaatc atcgccgtca cggtaataac actcggcgtc aaacgactaa
     5581 agccgtgggt atatttcagg ccaacggccc ataccacttc cagcagacca gcaataacta
     5641 agataatcca ggacatatca ggctccggag aattggggcc gtccccggtg aaaagaggcg
     5701 tttgcaggtc gtcctgcaaa gctgatggtt gaacggatat tttgccaggt ggcaggggaa
     5761 tttcaactat taccttagtg actatcgcag aaaaaacaaa cggcacgaca tacctgtcgt
     5821 accgttattg cttattgcta ttgctgcgct ttcgttgctg cgccagaaat cgcgttaccg
     5881 ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc aggcagttaa tactgttgaa
     5941 agcaccagaa cagaaaagat cgctgcaatt gtcttcttca ccataacgtc ttccttttag
     6001 gtaatgttat ttatgtttgc ctatagcaaa ttaataatag acaacatcgc caaaaatgac
     6061 ggaaatctga tgatttttcg gaagagaaga aaaattagct ggcagcgcga gagatggagt
     6121 tgccgagatg tttgatgtct tcgccgaaac cgcgagcggt gtt
//
LOCUS       DAZGJW010000728         3897 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.guided.274, whole genome shotgun sequence.
ACCESSION   DAZGJW010000728 DAZGJW010000000
VERSION     DAZGJW010000728.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.guided.274"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..183
                     /locus_tag="ACHKIK_005978"
     CDS             <1..183
                     /locus_tag="ACHKIK_005978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HFO9086844.1"
                     /translation="NRLQQLQIPLSDLQTALTENNENRGAGYIEKNGQQLTVRVPHSI
                     VNKTFLSFIFNGLFSC"
     gene            424..1239
                     /gene="sul2"
                     /locus_tag="ACHKIK_005979"
     CDS             424..1239
                     /gene="sul2"
                     /locus_tag="ACHKIK_005979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFO9086845.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1326..1628
                     /locus_tag="ACHKIK_005980"
     CDS             1326..1628
                     /locus_tag="ACHKIK_005980"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9086846.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(2012..2848)
                     /locus_tag="ACHKIK_005981"
     CDS             complement(2012..2848)
                     /locus_tag="ACHKIK_005981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFO9086847.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(2848..3651)
                     /locus_tag="ACHKIK_005982"
                     /note="aph(3'')-Ib"
     CDS             complement(2848..3651)
                     /locus_tag="ACHKIK_005982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFO9086848.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT          866 a         1112 c         1029 g          890 t
ORIGIN      
        1 aaccgattgc aacaattgca aattcctttg tctgatttac aaactgcctt aacagagaat
       61 aatgaaaacc gtggtgctgg ctatattgag aaaaatggac aacagctaac ggttcgtgtt
      121 ccccactcta ttgtaaacaa gacattttta tcttttatat tcaatggctt attttcctgc
      181 taattggtaa taccatgaaa aataccatgc tcagaaaagg cttaacaata ttttgaaaaa
      241 ttgcctactg agcgctgccg cacagctcca taggccgctt tcctggcttt gcttccagat
      301 gtatgctatt ctgctcctgc agctaatgga tcaccgcaaa caggttactc gcctggggat
      361 tccctttcga cccgatcatc cgtatgagac tcatgctcga ttattattat tatagaagcc
      421 cccatgaata aatcgctcat cattttcggc atcgtcaaca taacctcgga cagtttctcc
      481 gatggaggcc ggtatctggc gccagacgca gccattgcgc aggcgcgtaa gctgatggcc
      541 gagggggcag atgtgatcga cctcggtccg gcatccagca atcccgacgc cgcgcctgtt
      601 tcgtccgaca cagaaatcgc gcgtatcgcg ccggtgctgg acgcgctcaa ggcagatggc
      661 attcccgtct cgctcgacag ttatcaaccc gcgacgcaag cctatgcctt gtcgcgtggt
      721 gtggcctatc tcaatgatat tcgcggtttt ccagacgctg cgttctatcc gcaattggcg
      781 aaatcatctg ccaaactcgt cgttatgcat tcggtgcaag acgggcaggc agatcggcgc
      841 gaggcacccg ctggcgacat catggatcac attgcggcgt tctttgacgc gcgcatcgcg
      901 gcgctgacgg gtgccggtat caaacgcaac cgccttgtcc ttgatcccgg catggggttt
      961 tttctggggg ctgctcccga aacctcgctc tcggtgctgg cgcggttcga tgaattgcgg
     1021 ctgcgcttcg atttgccggt gcttctgtct gtttcgcgca aatcctttct gcgcgcgctc
     1081 acaggccgtg gtccggggga tgtcggggcc gcgacactcg ctgcagagct tgccgccgcc
     1141 gcaggtggag ctgacttcat ccgcacacac gagccgcgcc ccttgcgcga cgggctggcg
     1201 gtattggcgg cgctgaaaga aaccgcaaga attcgttaac tgcacattcg ggatatttct
     1261 ctatattcgc ggttcagcag gcatgtcccc tttgagggcg acccgacgac aggataatcg
     1321 accttatggt gcgcaaatat ttcggcacag acggtattcg tggcaaagcc aacgaaggcg
     1381 cgatgacggc ggaaaccgcc ttgcgcgtcg gcatggcggc tggccgtgtc tttcgtcgcg
     1441 gtgaccaccg ccatcgtgtc gtgatcggca aggatacgcg cctgtcgggc tatatgcttg
     1501 aacccgcgct cacagccggt ttcacctcga tgggcatgga cgtattcctt tttggcccgc
     1561 tgccgacaac gtataggaag aataaacgcc cttttcaccc aagtccaaca gctttggacc
     1621 gcagttgact ctttcgacac ccctgcgatg caacccaatc cggctgacgg ggagccagca
     1681 acgctgaaaa tttaccctcc tctttcccac tagcggctcc ttttccgaca accagcacgg
     1741 cggatccctg ccgcggcgct gtgaacgcag cattttgatt ggtatcgttg gccttcaggc
     1801 tcgtcagtca aacagaccca ggagcagctc agccggtggc gcccggcttt cgggtaacgc
     1861 cctggtcccg ctggtttcgg ctttgtgctt caggtgatca aggatggggt cgtttgcggg
     1921 agagggcgaa atcctacgct aaggctttgg ccaacgatat tctccggtaa gattgatgtg
     1981 ttcccagggg ataggagaag tcgcttgata tctagtatga cgtctgtcgc acctgcttga
     2041 tcgcggccgc gatagctaga tcgcgttgct cctcttctcc atccgcgttc caagctgcgg
     2101 aaaggcaccc ataagcgtac gcctggtcga gcaggcgacg cggatcgacg tccagcgcac
     2161 gagagaatgc gtccgccatc tgtgcaatgc gtctaggatc gagacaaagg tcgtctctgt
     2221 cagccggatc gtagaacata ttggcggcgc caaagcccac ttcaccgacc agaccgacgg
     2281 gatctatcac cagccagccg cgactggaga acatgatgtt ttcatgatgc agatcgccat
     2341 gtagcccacg cagttccgag gcattgctca tcatttgatc ggctataatc gccgcgtgga
     2401 cgtagtcagt ttgacaacct gcgttttgat catcgcgcgc ccgctgaaac aaagctgcaa
     2461 agcgatcccg gatcgggaga agggcagaag gcaggggttc ctcagatgcg gcatacagct
     2521 tcgccattag ttccgctgca atttcggtcg cctggtagtc gccgtgctcg gcaacgatgt
     2581 gagagagcat tcgctccccg gcatattcga gcaacatcag attgttctca cgaccgagca
     2641 accggactgc tcccctccca ttgcgccata ccagatagtc ggccccgcgc agttcatcag
     2701 caatgtcttc tataggtttc aatcccttga cgattgcagg agtcccgtct ggcaatgaaa
     2761 ctttccaaac gaggctggaa aaggtgtccg caatgagaac aggttgcgaa acgtgccaat
     2821 gagcaggaaa aacaggcggc atgaacatca accccaagtc agagggtcca atcgcagata
     2881 gaaggcaagg cgttcgcggt cgggggcttc gatccccaat acattgaata ggacagcgaa
     2941 ggcgcgctct gcttcatctg gcgctgccca gttctcttcg gcgttagcaa tcatgagtgc
     3001 caaatcggca tagcgatctg ctgttccgag ccgcccaagg tcgatcagac ccgtgcattg
     3061 aagagtttta gggtccacca tgaagttcgg catgcaggga tcaccatggc aaacaaccat
     3121 atcggtgcgc tcttggtcga gccgcaccgg tagctctcgt tcgacacgag ccaaaagatc
     3181 gagctgcggc gtactcttgt cctcgtccgg taagaagtcg ggattgacgg cattgcggga
     3241 caccacatca acggcgcgtc cgaacattcg cgacagcctg cgctcaaacg gacattgatc
     3301 aaccgatagg ctgtgaacag cgccaagttg ctgccccatt gacggccacg ctttgagcaa
     3361 atccgctcca gacagatcag ccgccggtac tcccggaatt gccgttatca ccaagcatgc
     3421 accctcctgt tcctcctgcc agttgatcac ctcggggcaa gccacacctc gacctttgag
     3481 ccaaatgagg cggtcacgct ctccagcgag ctcaccgcgg cgggaagcag gtgcgatttt
     3541 cgcgaaggca tgcccgtcac cacgtcgaaa aacaaaatca ccagattctc cgcctctgac
     3601 aggcaaccag tcagaatgcg attcaccaaa aaaaatatta gttcgattca atggaggttc
     3661 cttcagtttt ctgatgaagc gcggaggtgg ctcaacctgc gaaaagaaac gagttgctac
     3721 gtaagtccga gaacatgctt tccatggtct tgcaccggtt gtgttccggt ctatctctca
     3781 ttttaagcgg cttttttctc aaatgccacc ggcgatttcc agccgagtgc tgatgaatcc
     3841 cctaatgatt tttatcaaaa tcattaagtt aaggtagata cacatcttgt catatga
//
LOCUS       DAZGJW010000729         1833 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.guided.383, whole genome shotgun sequence.
ACCESSION   DAZGJW010000729 DAZGJW010000000
VERSION     DAZGJW010000729.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.guided.383"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..217)
                     /gene="tetR(B)"
                     /locus_tag="ACHKIK_005983"
     CDS             complement(<1..217)
                     /gene="tetR(B)"
                     /locus_tag="ACHKIK_005983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFO9086849.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEG"
     gene            299..1504
                     /gene="tet(B)"
                     /locus_tag="ACHKIK_005984"
     CDS             299..1504
                     /gene="tet(B)"
                     /locus_tag="ACHKIK_005984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFO9086850.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1617..>1833)
                     /locus_tag="ACHKIK_005985"
     CDS             complement(1617..>1833)
                     /locus_tag="ACHKIK_005985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFO9086851.1"
                     /translation="LEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLNGWFMHIAIHA
                     KNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          456 a          352 c          402 g          623 t
ORIGIN      
        1 ccccttctaa agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt
       61 cgagcaaagc ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct
      121 tctgggcgag tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg
      181 cgctgttaat cactttactt ttatctaatc tagacatcat taattcctaa tttttgttga
      241 cactctatca ttgatagagt tattttacca ctccctatca gtgatagaga aaagtgaaat
      301 gaatagttcg acaaagatcg cattggtaat tacgttactc gatgccatgg ggattggcct
      361 tatcatgcca gtcttgccaa cgttattacg tgaatttatt gcttcggaag atatcgctaa
      421 ccactttggc gtattgcttg cactttatgc gttaatgcag gttatctttg ctccttggct
      481 tggaaaaatg tctgaccgat ttggtcggcg cccagtgctg ttgttgtcat taataggcgc
      541 atcgctggat tacttattgc tggctttttc aagtgcgctt tggatgctgt atttaggccg
      601 tttgctttca gggatcacag gagctactgg ggctgtcgcg gcatcggtca ttgccgatac
      661 cacctcagct tctcaacgcg tgaagtggtt cggttggtta ggggcaagtt ttgggcttgg
      721 tttaatagcg gggcctatta ttggtggttt tgcaggagag atttcaccgc atagtccctt
      781 ttttatcgct gcgttgctaa atattgtcac tttccttgtg gttatgtttt ggttccgtga
      841 aaccaaaaat acacgtgata atacagatac cgaagtaggg gttgagacgc aatcgaattc
      901 ggtatacatc actttattta aaacgatgcc cattttgttg attatttatt tttcagcgca
      961 attgataggc caaattcccg caacggtgtg ggtgctattt accgaaaatc gttttggatg
     1021 gaatagcatg atggttggct tttcattagc gggtcttggt cttttacact cagtattcca
     1081 agcctttgtg gcaggaagaa tagccactaa atggggcgaa aaaacggcag tactgctcgg
     1141 atttattgca gatagtagtg catttgcctt tttagcgttt atatctgaag gttggttagt
     1201 tttccctgtt ttaattttat tggctggtgg tgggatcgct ttacctgcat tacagggagt
     1261 gatgtctatc caaacaaaga gtcatcagca aggtgcttta cagggattat tggtgagcct
     1321 taccaatgca accggtgtta ttggcccatt actgtttgct gttatttata atcattcact
     1381 accaatttgg gatggctgga tttggattat tggtttagcg ttttactgta ttattatcct
     1441 gctatcgatg accttcatgt taacccctca agctcagggg agtaaacagg agacaagtgc
     1501 ttagttattt cgtcaccaaa tgatgttatt ccgcgaaata taatgaccct cttgataacc
     1561 caagagggca ttttttacga taaagaagat ttagcttcaa ataaaaccta tctattttat
     1621 ttatctttca agctcaataa aaagccgcgg taaatagcaa taaattggcc ttttttatcg
     1681 gcaagctctt ttaggttttt cgcatgtatt gcgatatgca taaaccagcc attgagtaag
     1741 tttttaagca catcatcatc ataagcttta agttggttct cttggatcaa tttgctgaca
     1801 atggcgttta ccttaccagt aatgtattca agg
//
LOCUS       DAZGJW010000730         1111 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.guided.487, whole genome shotgun sequence.
ACCESSION   DAZGJW010000730 DAZGJW010000000
VERSION     DAZGJW010000730.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.guided.487"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            149..1108
                     /locus_tag="ACHKIK_005986"
     CDS             149..1108
                     /locus_tag="ACHKIK_005986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO9086852.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
BASE COUNT          294 a          238 c          265 g          314 t
ORIGIN      
        1 acagagcaat tgccttctaa gcaatcggtc actggttcga atccagtaca acgcgccata
       61 tttatttacc aggctcgctt ttgcgggcct tttttatatc tgcgccgggt ctggtgctga
      121 ttacttcagc caaaaggaac acctgtatat gaagtgtata ttatttaaat gggtactgtg
      181 cctgttactg ggtttttctt cggtatccta ttcccgggaa tttacgatag acttttcgac
      241 tcaacaaagt tatgtctctt cgttaaatag tatacggaca gagatatcga cccctcttga
      301 acatatatct caggggacca catcggtgtc tgttattaac cacaccccgc cgggcagtta
      361 ttttgctgtg gatatacgag ggcttgatgt ctatcaggcg cgttttgacc atcttcgtct
      421 gattattgag caaaataatt tatatgtggc cgggttcgtt aatacggcaa caaatacttt
      481 ctaccgtttt tcagatttta cacatatatc agtgcccgat gtgacaacgg tttccatgac
      541 aacggacagc agttatacca ctctgcaacg tgtcgcagcg ctggaacgtt ccggaatgca
      601 aatcagtcgt cactcactgg tttcatcata tctggcgtta atggagttca gtggtaatac
      661 aatgaccaga gatgcatcca gagcagttct gcgttttgtc actgtcacgg cagaagcctt
      721 acgcttcagg cagatacaga gagaatttcg tcaggcactg tctgaaactg ctcctgtgta
      781 tacgatgacg ccgggagacg tggacctcac tctgaactgg gggcgaatca gcaatgtgct
      841 tccggagtat cggggagagg atggtgtcag agtggggaga atatccttta ataatatatc
      901 agcgatactg ggtactgtgg ccgttatact gaattgccat catcaggggg cgcgttctgt
      961 tcgcgccgtg aatgaagaga gtcaaccaga atgtcagata actggcgaca ggcccgttat
     1021 aaaaataaac aatacattat gggaaagtaa tacagctgca gcgtttctga acagaaagtc
     1081 acagttttta tatacaacgg gtaaataaag g
//
LOCUS       DAZGJW010000731          885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2003F36963
            SAMN43267211-rid23071753.guided.521, whole genome shotgun sequence.
ACCESSION   DAZGJW010000731 DAZGJW010000000
VERSION     DAZGJW010000731.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267211
            Sequence Read Archive: SRR30307729
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351046.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351046.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:41:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,889
            Genes (coding)                    :: 5,603
            CDSs (with protein)               :: 5,603
            Genes (RNA)                       :: 99
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 125 of 286
            Pseudo Genes (incomplete)         :: 161 of 286
            Pseudo Genes (internal stop)      :: 68 of 286
            Pseudo Genes (multiple problems)  :: 58 of 286
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267211-rid23071753.guided.521"
                     /serotype="O 26:H11"
                     /isolate="2003F36963"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            31..300
                     /locus_tag="ACHKIK_005987"
     CDS             31..300
                     /locus_tag="ACHKIK_005987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFO9086853.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(598..>885)
                     /gene="iraM"
                     /locus_tag="ACHKIK_005988"
     CDS             complement(598..>885)
                     /gene="iraM"
                     /locus_tag="ACHKIK_005988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO9086854.1"
                     /translation="ACDISFSAIWCNIKLILWYQSDIFLPPGCIFTPTRTGVILNNKE
                     LPVTIYNVTPFNKNVWNLIKSSQECPANTGNITDKCFNHRCILEICPYGRK"
BASE COUNT          243 a          160 c          225 g          257 t
ORIGIN      
        1 atatacaacg ggtaaataaa ggagttaagt atgaagaaga tgtttatggc ggttttattt
       61 gcattagctt ctgttaatgc aatggcggcg gattgtgcta aaggtaaaat tgagttttcc
      121 aagtataatg aggatgacac atttacagtg aaggttgacg ggaaagaata ctggaccagt
      181 cgctggaatc tgcaaccgtt actgcaaagt gctcagttga caggaatgac tgtcacaatc
      241 aaatccagta cctgtgaatc aggctccgga tttgctgaag tgcagtttaa taatgactga
      301 ggcataacct gattcgtggt atgtgggtaa caagtgtaat ctgtgtcaca attcagtcag
      361 ttgacagttg cctgtcagac tgagcatttg ttaaaaaaat ttcgcatggt gaatccccct
      421 gtgcggaggg gcgactggtg tataaggtgt catctctgac gacaacctgg atcgagatac
      481 gcgggttcag ggacaccgga ctgaactcac cgggaggcac ccggcaccat gttcatggtg
      541 atacagaaat gcggcttcag cccctctccg gaggggcttt ttatctgaat gattctgtta
      601 ttttcgcccg tatgggcata tttccagaat gcagcgatga ttaaaacact tatctgtgat
      661 atttcctgtg tttgcagggc attcctggct gcttttaatt aaattccaga cgtttttatt
      721 aaatggggtc acgttgtaaa tggtgacggg gagctcttta ttattgagta ttacgcctgt
      781 ccgggttggt gtaaaaatac agcccggagg gaggaagata tcagactgat accataatat
      841 taattttatg ttacaccata tggctgaaaa tgatatgtca catgc
//
